LOCUS       DAOPPA010000001        29369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_2043_length_29369_cov_4.122774, whole genome shotgun sequence.
ACCESSION   DAOPPA010000001 DAOPPA010000000
VERSION     DAOPPA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 29369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29369
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_29369_cov_4.122774"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PKH54_00005"
     CDS             <1..1180
                     /locus_tag="PKH54_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOC98296.1"
                     /translation="SPFTRIVTGHDGGMDDHVFFATAASGTEPCLRDELRAMRLRGVR
                     CDRGGVHFSGSWEAAWRACMHSRVALRVLVELGGFEVTDGQSLYDAARTIDWRAYLTP
                     ELTLAVSAVCRDSELTHTNFIAQKIKDAVVDRIRDEVGTRPNVDRHDPDVAIFAHLVR
                     NVLTLYLDMSGESLHKRGWRADHLEAPLKENLAAAMLMLSGWDRRTPLHDPMCGSGTI
                     PIEAALWASNIAPGLSRERLGFERWANFDGQMAARMAEIRQEARDMIVTKALPFITGS
                     DIDQRAVEMAAANAMKAGVKVEFARCDVRDMMPLVSPGFVMVNPPYDVRLESNREFYQ
                     AMWRRFEALSGHVVCVLAGDPEVDYPSRIKPSRYQYLYNGDIKCRFGVFEIPARPRGR
                     "
     gene            1199..2824
                     /locus_tag="PKH54_00010"
     CDS             1199..2824
                     /locus_tag="PKH54_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98297.1"
                     /translation="MKNVLSVLALVALVAFAGCHNKRADQVKTPDGAAVAAQADVVQP
                     AVRKLSVDLHVMSQCPYGVQAENILIPIAEELRDNIDLKIHFVGDEVEAGVPESLHGE
                     TEVKADLLQICTGKHQPEFMFGMIICMNQNPDEIPANFDSCAVELKMPVETVRACADG
                     PEGKILLLESFKYSDEKGVKGSPTIFIDGTEYEGLPTVLGLLRGLCEKIGQPAPETCA
                     SLPPPVSIQLTVITDSRCPDCAKFVEVAPAQIRKMFPGVETVVLDYMTDEGKEAYKVL
                     AKTPFRFLPAFLFEKKVQEDPAFAQIAAYMMEVGEQLLLNFDASFDPEAEICDNGTDD
                     DGNGKADCKDDSCKGTLFCAVEKPKKLEVFVMSQCPYGAEAILSMREVLEAFGRDIDF
                     SIRFLASEPFAGQFQSLHGPAEVAEDMRQLCAIKYYAKGRKYLDYLYCRAADFQSEEW
                     EKCATGGIRAEVIRKCAEGDMGRKLLSADVAYGEKLRINGSPTWIVNNREVFNGVSPA
                     DIQKGFCKKNGTLKACETPLSDKAGVPAGACGN"
     gene            complement(2889..3539)
                     /locus_tag="PKH54_00015"
     CDS             complement(2889..3539)
                     /locus_tag="PKH54_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98298.1"
                     /translation="MQILISVSWLALLAAIIAALMVEPAAGVLVGLTKAHPYLMGFAK
                     FVLLGTMGELLGGRIATGRFRLRGIRLWQRALVWGFIGAMLALVFPLFSAGVEDLLAK
                     GLLPGLGFPLLHAFWKSALMNVLFGFEMMVFHRFTDTLIAEGRLFGPWQLVQTYRSID
                     WTSMFRIVGGALFWFWIPAHTVTFMLPPEYRIAAAALLGIALGLILGFASRKSRQR"
     gene            3628..6207
                     /locus_tag="PKH54_00020"
     CDS             3628..6207
                     /locus_tag="PKH54_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98299.1"
                     /translation="MASKKFPAALAGLILLVGVGCGGGESGTDVVDDAGADIAGDIQA
                     DVAGDIPSDIPSDGTADVAGDDGVAPDIHYWQGPQPLSLAGGIYDQVPSVFSRISRFV
                     TEVDGPEPVDPSFLGGAGVGNGLVFAMFGYGNPLNSMHSFAGPGYDLGDEFFGDYSVG
                     LAVGGATETAAFDRQVVAITADAPVVVARGTVGQIELHVVSFAPWFDGAVTPGSDSDV
                     LRHCIMRVVEVRNPGPAAPVVVRVLAHGDVESPQAGRMLEEDDGRVLVTAAAGATAQV
                     DGPVMAVDLGEMGEGASRSFVVTHCAFAAGQIVELPEFDAAALLARTADAYRKWESEL
                     VDVETPDPMVNDFIDGMKLTLKLQTSDGGATCPMSEYTRTWARDNMGPVLAMLSLGGF
                     ADVEAYLDYVYYAILKEGDLKNSYPANLEIDWDGVVAPDWDSMPPLGEGSVGKKRVSA
                     ETPSYMVLMYGLHNRFTGKTDRIVERLGLLRRCLFAQGFSDGDMLPFTGDETFRAAMN
                     AALGLGLEEPHEDTNWSGNSSALWLGAAREYARFMEQAGQDAESAMAQERFEVVEAAT
                     LAAYTRPDKCFAALVSMSDMAMSKPFEDASLQVTWSGWKDGDDPYARDAFSCLYETAR
                     TVPGEICSKLDTMYGGVFRDAWDGVYTGMMPGYALHSMTVLGHPDAFAAFNMVRKSLD
                     TLGNVQEYLILDDHSGLTLMYNDDGVTTPSDYTAKYRPWEGGIVLDAVLQFLFGFEPD
                     ANTQRLELRPHVPNGWPQMSAKGLRAASSRFDVSVSSSGTVSRVTVTSRADADWIVSL
                     RWDDPVPGVRGPTFTLDGGVEPLQGVTRIDTPWMPTYLLPDLVLKAGESLQIVVTGCG
                     CGG"
     gene            6335..7669
                     /locus_tag="PKH54_00025"
     CDS             6335..7669
                     /locus_tag="PKH54_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98300.1"
                     /translation="MKGYLLAAAALVPIVLSGCAGESGVQDVVDAVDNVDVMADAGDV
                     PGDENAVGPDIDERVDSSSDVCVPFCPAWAECGDDGCGGSCGTCWWEEWCEEMHCVHV
                     DPPPDCDGIECGYNEDGYLCGECDEGMECIDGHCGFVDYPDCTNRECGDDGVGGSCGQ
                     CDKGFICNLWGECEPTCEYPQDLPAGWGIVSVVDFLTIPDTTEEKAGCFDYTDDGVPD
                     NHLSGLASQFQTVLDDMGTWTTNMAMEFRPVKAIIADPGITIDGMRVRHDDAGQPGTF
                     LADPVSWNPDNCRPFIRFDDVDVEEGDMSAGTQEITVHINVSPSFMLPIRLIDVHMSG
                     ILQPMGEGIEVTEGVISAVAMGSDLNQAVNLLWVECDKEPPPEDLVETCQALQMAMAP
                     QPRIVGPGFLLQLHRTADGTYLDRDSEHPGNAMAMCMKFSAKPGTITGYFTP"
     gene            7745..9070
                     /gene="xseA"
                     /locus_tag="PKH54_00030"
     CDS             7745..9070
                     /gene="xseA"
                     /locus_tag="PKH54_00030"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOC98301.1"
                     /translation="MDLEEFVGSVGSGETDAVMSVIVVSSRIKTLLEGTFRRVSVEGE
                     VSGFKPAMSGHVYFSLAERDAKGSTQKLDCVIYRFAGAGRAPILKDGDRVVATGRITA
                     WAGASRYQMSVDSVRLSGSGNLLRQLEELRSRLMSEGLFDPARRRPIPSFPRRIGVVT
                     SIRGAAVRDIARTILSRWPADILVVDSLVQGDGAAEGIARGIEMLNRVPEVDVIIVGR
                     GGGSMEDLWAFNLEKTVRAVAGSRKPVISAVGHEIDHVLTDDVADARAATPTAAGQMV
                     VPSRDEIQVSIDDAASRMGRAVARRIDESAQSMDDAAEMLAAAGRRMLDARERLLESR
                     RARLAAGHPARRLMDKSRHLETAGVRLSSAGKRLLERPAANLERLRLRIESFNPALPL
                     DRGFAIVTTSGGALVRGPSQLKQGDVVTVRLKKGAVDAQVTSVRPENGG"
     gene            9075..9617
                     /locus_tag="PKH54_00035"
     CDS             9075..9617
                     /locus_tag="PKH54_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98302.1"
                     /translation="MEATAGWAIDFIRKIVARAPLRPPASPGELVAQQMVEDELKAIG
                     LQTSWHRFRFHRSIYGNFALHFGLAVAATAVSPWCPIGAAVVHALVAFSYAMQSTRRH
                     SVLSRLYFRRPSQNLLAVMPAEGGRPAIAAPGLHGARRRRFQRACLQSGIRSLDHEFK
                     GSVQALDCPCDVGDRGDGGV"
     gene            9544..10347
                     /locus_tag="PKH54_00040"
     CDS             9544..10347
                     /locus_tag="PKH54_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC98303.1"
                     /translation="MNSKGPFRRSIALATWATAAMVAFDLLRVFADPLPYVRFVEWAV
                     TAPAIIAFLLNFDVYVRNRHVQGANDNLTAAAAMPVLAARLGPERPPDVELVFALTGC
                     EEAGLGGAMALQREVRSRWDRKNTVFINMDGLSNGQLRYIDPEGELFLLRPDRWLVDL
                     VDEVRGSRPGFAPFADVAPFDITIGGTDMAAFQTAGWQGISFVAIDPATGSPRHYHTT
                     RDTVDNLDLEEYEVSLDFISELARRVMKRGSSAVKNEETAADPRPSPIS"
     gene            complement(10298..11062)
                     /locus_tag="PKH54_00045"
     CDS             complement(10298..11062)
                     /locus_tag="PKH54_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC98304.1"
                     /translation="MRILFIGDIHGDIRSTWEMLDAARRELRIGAAVQVGDFGFFPEI
                     LGNLPDDFPRFPVPLYAIDGNHEDHAWLAACRENRTVEEWKRRLDLHYMERGSVTVIG
                     GTRFGFLGGALHVDRPQEFDSASGSSNYIQQDDVASALERFRNERPDVIVTHSCPTGI
                     GIGIHGSPIFLPGVLAFISSAGFDAGVEGDCGDSELTRLWNGLETRPKAWIFGHFHTD
                     ILRRVHDTEFVCVAGIDSRTVTIWDSDEGRLLFLHF"
     gene            complement(11069..13540)
                     /locus_tag="PKH54_00050"
     CDS             complement(11069..13540)
                     /locus_tag="PKH54_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOC98305.1"
                     /translation="MKRKPGHVRYHWKSFLINILSPVILAVILFNVLIFAIVIPEMKD
                     NLIERKHELIRELTRAELSEIEALYQQQADGIIDRETAQKTALSRLRSVRYGDEGKDY
                     FWVTDSHPRMILHPYRPDLEGQDLSDYSDPRGKRLFVEMARVVLDAGEGRVDYLWQWK
                     DDSKKIVPKVSFVKRFEPWDWVIGTGIYIEDVRAEVDKVTRKVINISLGISLLVALLL
                     LYMIRGSLAIERQRSLTQAALRDSEEKYRTLVESNTEGIMLAIDGKPVFCNRALLDMT
                     GHSAAAFTSLTMDEIIGKDSKLIARDGKTIDVIISNTPVTMGGRAGSLISFKDIGRGR
                     ANQPAVTKLLSDLQTSLLLSNSSIGATGLNTLECTLDTSIQQAAAMMTARGVDAIMVR
                     TNADEPVGIVTDTDLRQRVLVQHRSPADSVASVMTAPLAVINEGSLMFEAALLMKEKG
                     IRHLAISGDRGRISGILSLGDVVLAQRHPAAIILKNIQNASSAGELTGVRGKVNALVS
                     AMLENGTAPDAATRMMSGIADASIRRIIELALERLGPPPAPFAFIVLGSEARREQTFR
                     TDQDNGIIYSPVHDREEEVSAYFLKLGAAVNEDLDQIGYLKCRGNVMAGNPKWCRPLH
                     DWQRYFLECVSSQTPQALLDFNVFFDFRHVFGDEMLVFLLRDHIHRIVADRHAFFFNL
                     AESTLQFKPPIGFFGNLQLESSPESPAAFNIKNALTPMVNFARIYALKNGIDECNTLA
                     RLTRLREAGILHKSSCEEYVQGYSFLMRLRLVHQARLLLGGKEPDNLIDLESLTQLEL
                     STIKKIFADIVVMQARLRNDFARTA"
     gene            complement(13621..14802)
                     /locus_tag="PKH54_00055"
     CDS             complement(13621..14802)
                     /locus_tag="PKH54_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOC98306.1"
                     /translation="MRKASLVIFLALACISMPARAIKLEQSKDMFLNINLLLQFQAQL
                     IQEGAPDQKSWGKEFFLRRVRLLLGGQVNKYISFFIDTDMPNWGKGNDWNKPTFFIQD
                     AFLTFKVVDQFMVDAGLIIAPFSRHGYQSAVSLFGLEYHSDLIKYPTEATKVWRDAGA
                     QIRGYIFKEQLQYRFSVMNGSKNDVLQKDINDKAVVLTNPKDYPRVTAHLRYNFLGTE
                     TDMFAKGIHFADKPIVSLGVSFDYQQDAGIDRLAEFEKTAAGKDTAIIAKDGKLGDYM
                     GVSADLYAEVPFAGKDHEFILMADFYRYWQGKASAASGIGLFGEFGYRYKTFSPIFGI
                     DWFKSDLPDKDLLVIHGGLVWWAVKHNANIKLDAAVKRQGDVLDSPFMTMITLQGQIY
                     F"
     gene            complement(14820..16721)
                     /locus_tag="PKH54_00060"
                     /pseudo
     CDS             complement(14820..16721)
                     /locus_tag="PKH54_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629154.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
     gene            complement(16724..17011)
                     /locus_tag="PKH54_00065"
     CDS             complement(16724..17011)
                     /locus_tag="PKH54_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOC98307.1"
                     /translation="MLHEPAVQCEKDFAASYKQRLGVWMFLLYALVYAGFVAINLIDP
                     AMMESRIFFGLNLAVVYGVALILFALILAVIYNLMCLRRERLAAESNEGGN"
     gene            complement(17273..19312)
                     /locus_tag="PKH54_00070"
     CDS             complement(17273..19312)
                     /locus_tag="PKH54_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98308.1"
                     /translation="MDLSGESRLIRIAAGCSLGCSFCTVGQGREGVCPDDAVAGRFEI
                     PEASRYLLLAGNLLDRRFAPTVSRLAEAGADKIDAYVHCGPADPAALKDLAAAGLTGI
                     QLVLPSAEREMLAGLTGGRGSLKMAAMILSGARELGLEVTLQIPVMKANAESIDDTVA
                     RASRLCMPARVTLDFVSESRRDGTPNKWDPAIAIESVTRTIKFCQDNGVIFQIGRNEA
                     PPPCIFPLKNVPALAWGDLAGIPSDGNIARSKAPFEACAACDAREICTFSGRHLEPST
                     PPRPLRGGQGGDQDQVWLDAAAIWVRRNELRTLRQELARRHPICMNPWTNLETHSVRG
                     VAISCATHRFQYGTVDQCVSWHDVSLAQAWNSDVMQSLRHQMKIGQPHLHCRPDCVVF
                     HGGGPGDSWLTADPLTDVFHRNLVTNLKEIIDGAEILESMPQNLVIAPSLHCNIACIM
                     CRFPGEVRDAPDPSKTMMSERQFDEVIEMMPYLRVLNITGGEPLIADGTRRLLKAFKT
                     DRFRDGGIALTTNGILLDKSLISDMKKSRLLSIIISVNAASRETYRHVTLVDAYDRVI
                     NNVTELMRARREFNPLPRVVLSFVIMKCNFREIPDHIRTAARLGTDIQLLPMVGKLGG
                     ESMFMCRQDLQELLDFVDREVVPLVAGMKGNFRREVTTLRSTMSRRIKDDDYSMF"
     gene            complement(19326..21368)
                     /locus_tag="PKH54_00075"
     CDS             complement(19326..21368)
                     /locus_tag="PKH54_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98309.1"
                     /translation="MNREESGIVFVGRGCGCGCLSCTMSEGRIGPAPASLEIRDLPVC
                     HRATIMAGDLIHEDVVPIIKSLRELGSEEVFLYVHPACNDVTLLKTMAESGLTGLHVV
                     IPASDRATLAKLCGARARLESIALLLKAAASLHLDVTAELPVTQESLPGITDTFRRVK
                     GLCPTIRKVALPFRATHGPASAGAWDFRLASEPIREIAAMAHAAGISLTIGGFEGPAP
                     CVIDIEDAKVEHYPGLDGSPGLLRERGAAVAASCASCGIRKSCNMGMHAFAGKAPDPI
                     AWVDPDRSPEPGVPVASGLSRCRTTTLFSRRERLDSLLARAATIDRCMAPWDSIEVHD
                     RTGRVAPCEGSWPRREILGTPDGTCDGWLTDGILKSFQGDRMTSMRRLMVDGGRQATC
                     NRHCPRFAESWSDLPPVRLPRTEVFDSNLALGLSEFIEGSASVKSRPRNITISPSLKC
                     NQRCRMCDLTDEDAGATGIDTPPALLDELVELLPTTATVALTGGEPLTSGRIVKILQS
                     ITPERLPDTSYTLTTNGTLLTPALIERLRPTSMARIIVSINAATAATHELVTGTRGTF
                     DKVIGNVAAMVAAAGGMPRKPTVTASFVVMKSNLGELGAFIELAARLKTEVRLLPIER
                     DRMGESFFTDEASKKHAECVVRSLLDRYMLVWPHAARQLEGLLSVLRMRSGMTDPL"
     gene            complement(21473..23140)
                     /locus_tag="PKH54_00080"
     CDS             complement(21473..23140)
                     /locus_tag="PKH54_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98310.1"
                     /translation="MSVTTSSRYVRRAHRPRVVATIRSAGSAAPFVAALLLACGVPSV
                     GEWEEYGADRDIPVSDAGNAGKPDIQTPKSCTTNDDCGDQALHSRMCDVPICDLGYCS
                     TRRQQVGHPCDDSNATTVMDSCRADGTCAGTQYLCGDHICTDSFENCTTCPADCGCGA
                     NMYCRDGDCLSIPEDEDGVCEKLEDCATSPVDCACPASHACIDGSCMDCSVACAQDHR
                     NCGEFEGCDCGRCTDGLVCGNWGQCAVPGDCGDGECLKDFENCLDCPEDCPCQKGYGC
                     TSAGRCVSCRTSCQGIECGTVDGCDCGTAGVCQRCAAGRIVTDCQCVCSTAGRECGNI
                     GECTCGSMNGGCMDRMKCENGLCMPDCPKVCDDIECGAIGNCLCARCPASQVCYDGGC
                     ATVTFDKDVYEPDNYPGQAAYLGEFTDGDNSTDWDVIANILEPEDRDWYEATVEDTWG
                     EYLAVDVRLDGMSEDADLNLMVCYRCDRGKLTEPDLDPSGDVFETVSSIEGARCFESR
                     RRWGIAEQISFDPVCSGGFDDSGTVFVKISPAQRDDARTDYLLAISF"
     gene            complement(23225..24070)
                     /locus_tag="PKH54_00085"
     CDS             complement(23225..24070)
                     /locus_tag="PKH54_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOC98311.1"
                     /translation="MRPDVTRLKPFLDAVYDRFHTEESLACDPLSFPRQYRDPIDREA
                     AAVIASAFAFGRVAAFMPVIDKVLSRLGESPGQALAEGNRALFREVADGVIYRFATPP
                     NVEALLSGTAALLRRDGSLEPSVMSGFAAGGTVNGLLSLADALRSSGDGDPGFLVPVG
                     NPDSPMKRLCMLLRWMVRRDGIDLGIWNGLRPSDLLFPLDVHVFRISGLLGLGDAGQN
                     NAPRMKDAIALTRQLAELDQEDPVRYDFALSHLGISGTCRGSAGPNCADCPLVTVCGA
                     RLACD"
     gene            complement(24055..25980)
                     /locus_tag="PKH54_00090"
     CDS             complement(24055..25980)
                     /locus_tag="PKH54_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC98312.1"
                     /translation="MGKASRRLRQGSATATRTEPAPVSAAMVGVLAAVMPILSVLPYG
                     PFHVLPAVTRIPLAAILSLSMLSGIPRLARFNGRRAALALEAAGLAVILGTWFLLKLV
                     GLHPSGTDDNIYFYLANRVAEGAVPYRDFFFSHPPVHLLVPAAVFKIAGYSIGAAKLI
                     PVLAQTIAGLFMYLTLRPVSRPMALVALFAHFSTYQILMGSTDMNGENIMTMFLWAAA
                     WAGFRRFPVVAGVLAGLAIGTGMYALAGVLAVGIAILVADRKAAVRYLIGFIGIFALM
                     LLIFGISGGKSFFDGVFLYHTRKAVSAEGRQSLFSSPNPFRMAVILISNLTAFLKGPT
                     FLKTLYYHGAMFLLLLPATWMAVREAIRGFRTDAFKPHFIALSSVAATALFLLQWAAV
                     NEVYDFYLVPMLSFMCPAVGYAVDQAVSRMKRASGASSLAFPLAVLAAAWLAVPWSAH
                     LNAALWPEESKLKGQKVAYEWRDPAILKGPAGLTKALFFADSRERGAITPYYRHYIWN
                     KSLAFDSVDEIADFVRQNTIPEQTLTGASTLAPLVALHAGRRISADEADTNGKRFSSG
                     MLSDAGFIDQVCADKVAFVVSASGSYFPFDKMTSAEPFKSRFQPARTFNEKKLKHFKD
                     FPITLFKTRENIECGRM"
     gene            26106..26459
                     /locus_tag="PKH54_00095"
     CDS             26106..26459
                     /locus_tag="PKH54_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOC98313.1"
                     /translation="MDSRKAEGLRIEELVAGELVRRGYRIIARNYRKPWGEIDIVAIS
                     GRQIVVCEVRSRSRGVAADAAESVGIEKRKKIRMTTESYLQECPFLFDEVRFFVAAVV
                     MSAGRPEIEIIEDAF"
     gene            complement(26475..27128)
                     /locus_tag="PKH54_00100"
     CDS             complement(26475..27128)
                     /locus_tag="PKH54_00100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOC98314.1"
                     /translation="MIQTQLLNDLPSGPGFIEDWARKGGFPSIAGVDEAGRGCWAGPV
                     FAAAVILPERFDLPDLDDSKTLSHADRDALYSPIKEQAVAWAVAQSSCQDIESLNILG
                     ATLKAMKAAVEQVCARVMPSIVVVDGTNIIPGLAMPQRAWVRGDHLSYAIAAASILAK
                     VSRDRLMEEMDLVYPGYAFSSHKGYGTAAHRAALDRLGPCPIHRRGFKPIAAMLRSR"
     gene            complement(27103..27501)
                     /gene="rplS"
                     /locus_tag="PKH54_00105"
     CDS             complement(27103..27501)
                     /gene="rplS"
                     /locus_tag="PKH54_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOC98315.1"
                     /translation="MHQTIQMLEQIHRRGDLPDFHPGDTVKVFVRVREGDKERLQMFE
                     GYVIGRKGNGIHETFTVRKISYGIGVERIFPVHSPMVSRLEVVARGRVRRAKLNYLRA
                     LKGKAAKISERKTWLVGAVSKTDDTDSASE"
     gene            complement(27598..28179)
                     /locus_tag="PKH54_00110"
     CDS             complement(27598..28179)
                     /locus_tag="PKH54_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC98316.1"
                     /translation="MNKPVCQIAAALVHHPVYDRIGEVVATSVTSVDIHDIARACRTY
                     GLNRYYLVTPILAQREMVDRVAGHWSEGAGGKAGHPRGPALELVRTVHDLQSAIEDFQ
                     LTFGIRPQVAVTSANPMRDATTFADLRKTIESGKIPGLLIVFGTGWGLANSIIEKSDI
                     RLEPISGPTEYRHLSVRAAAAITLDRLMGVSIY"
     gene            complement(28172..28999)
                     /gene="trmD"
                     /locus_tag="PKH54_00115"
     CDS             complement(28172..28999)
                     /gene="trmD"
                     /locus_tag="PKH54_00115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOC98317.1"
                     /translation="MTDFLPSSPVPFRFRILSLFPQMVREAAAHSIIGRAATAGLIDI
                     DAIDIRDFTTDRHRTADDIPFGGGPGMVMKPEPVAGAIRAAKELMPDALVVLMSASGR
                     LFDQAMATRFASHSGGLILVCGHYEGVDERIAEHFCDMEVCVGDYVLSGGELPALTVM
                     DATARLIPGVLGNVESLQDESHNDSMLECPQYTRPRDFEGHLVPDVLFSGNHAKIARF
                     RRECALAKTRANRPDLVDRLEPETAPEAPRESAHDNGQAPLTANPGIPTGWGRPVDE"
     gene            complement(28996..>29369)
                     /gene="rimM"
                     /locus_tag="PKH54_00120"
     CDS             complement(28996..>29369)
                     /gene="rimM"
                     /locus_tag="PKH54_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOC98318.1"
                     /translation="GKSTVMTVESIRDKGKFLIVGLAGCTDRNAAEKLRGAIVCCDRD
                     DLPELAPDEFYYRDAIGLPVMFPDGQVVGKVSQVLSLATDVIEITSDSGQEWMIPVVE
                     GFVRRMDRDAVIIEPDALEME"
BASE COUNT         5731 a         8594 c         9221 g         5823 t
ORIGIN      
        1 gtcccctttt acgcgcatcg ttaccggcca tgacggtgga atggacgatc acgtcttttt
       61 cgccacggcg gcaagcggca cggaaccttg ccttcgggac gagcttcggg caatgcgtct
      121 tcgcggcgtc cgttgcgacc gtggcggcgt gcacttttcc ggctcgtggg aggccgcgtg
      181 gcgtgcgtgc atgcactccc gggtcgcgtt gcgggtactg gtcgaactgg gcggcttcga
      241 ggtgaccgac ggccagtcgc tttacgatgc ggcacgaacc atcgactggc gcgcgtacct
      301 tacgccggaa ttgaccctgg cagtttccgc cgtgtgccgc gacagcgaac tgacccacac
      361 gaacttcatc gcgcagaaga tcaaggatgc ggtggttgat cggatccgtg acgaggtcgg
      421 aaccaggccg aacgtggatc gtcatgatcc ggacgtggcg atttttgccc acttggtgcg
      481 caacgtcctg acgctttacc tggacatgtc cggcgagtcg cttcacaagc gcggctggcg
      541 cgcggatcac ctggaagcgc ctttgaagga aaaccttgcg gccgcgatgc tgatgctttc
      601 gggctgggac cgcaggacgc cgttgcacga cccgatgtgc ggatccggga ccatcccgat
      661 cgaggcggcg ctgtgggcgt caaacattgc gccggggctg tcgcgggaac ggctgggttt
      721 cgagcgctgg gcaaacttcg acggccagat ggccgcgcgg atggccgaaa tcaggcagga
      781 agcccgcgac atgattgtta cgaaggcgct gccgttcata acgggatccg atatcgatca
      841 gcgggccgtc gaaatggccg cggccaacgc gatgaaggcc ggcgtcaagg tcgagttcgc
      901 gcgttgcgac gtgcgcgaca tgatgccgct tgtcagtccc gggttcgtga tggtcaatcc
      961 gccgtatgac gtccgcctgg agtcaaaccg ggagttctat caggcgatgt ggcgccgctt
     1021 cgaggcgctg tcgggacacg tcgtctgcgt gctggcggga gacccggaag tcgattatcc
     1081 ttccaggatc aagccgtcac ggtaccagta cctttataac ggcgatatca agtgccggtt
     1141 cggcgtgttc gagatcccgg cgcgcccgcg tggtcgataa gccggtctgg aggattcgat
     1201 gaagaatgta ctttcggttc ttgccctggt ggccctggtc gcttttgcgg gctgtcataa
     1261 caagcgtgcc gaccaggtga agacgcccga tggcgccgcc gtggccgccc aggccgatgt
     1321 cgtccagccg gccgtccgca aattatcggt ggacctgcac gtgatgagcc agtgtccata
     1381 cggagtccag gcggaaaata tcctgattcc gattgccgag gaacttcggg acaacatcga
     1441 cctgaagatc catttcgttg gcgacgaggt cgaggccggc gttccggaat cgcttcacgg
     1501 cgagaccgag gtcaaggcgg atctccttca gatttgcacc ggcaagcacc agcccgaatt
     1561 catgttcggg atgatcatct gcatgaacca gaatccggac gagataccgg ccaatttcga
     1621 ttcgtgcgcg gtcgagctga agatgccggt cgagaccgtc agggcttgcg cggacggccc
     1681 ggaaggaaag atcctgctgc ttgagtcgtt caagtattcg gacgagaagg gcgtcaaggg
     1741 atcgccgacg atatttatcg atggcacgga gtacgaggga ctgccgaccg ttctcggact
     1801 gcttcgcgga ctgtgcgaaa agatcggtca accggccccg gagacgtgtg cgagccttcc
     1861 cccgccggtc agcatccagt tgaccgtcat cacggacagt cgctgccccg actgcgcgaa
     1921 gttcgttgaa gtggcgccgg cgcagatccg taagatgttt ccaggggtcg agacggtcgt
     1981 cctggactac atgaccgacg agggaaaaga ggcgtacaag gttcttgcca agacgccttt
     2041 ccgattcctg cccgccttcc tttttgaaaa gaaggtccag gaagacccgg ctttcgcgca
     2101 gatcgcggcg tacatgatgg aagtgggcga acaactcctg ctgaattttg acgcctcgtt
     2161 cgatccggaa gcggagattt gcgacaacgg caccgatgac gatggaaacg gcaaggccga
     2221 ctgtaaggac gacagctgca agggaaccct tttctgcgcg gtggaaaagc cgaagaagct
     2281 ggaagtcttc gtgatgagtc agtgtccgta cggcgccgag gccattcttt cgatgcgcga
     2341 ggtgcttgag gctttcggca gggacatcga tttcagtatc aggttcctgg cgtccgaacc
     2401 gttcgcgggc cagttccagt cgttgcacgg gccggccgag gtcgccgagg acatgcggca
     2461 actctgcgcg attaagtatt acgcgaaggg tcgtaagtac ctggattatc tgtactgtcg
     2521 cgcggccgac ttccagagcg aggaatggga aaagtgcgcg accggcggca tcagggccga
     2581 ggtgatccgc aagtgcgcgg aaggcgacat gggccggaaa ctgctttccg cggatgtggc
     2641 atacggcgaa aagctgcgca taaatggaag tccgacatgg atcgtcaaca accgtgaagt
     2701 gttcaacggc gtaagtccgg ccgacatcca gaaagggttc tgcaagaaga acggcacctt
     2761 gaaggcgtgt gagacgccgc tttccgacaa ggccggcgtg ccggccgggg cttgtggcaa
     2821 ctgagccggt aaatgccggc cataacggtg ccggccgttc ccccgccaat catgccgcgc
     2881 ttttcaagtc accgctgcct tgatttcctg gaagcgaaac cgaggatcag accgagcgcg
     2941 atgcccagaa gcgccgccgc ggcaatcctg tactcgggcg gcagcatgaa cgtgacggta
     3001 tgggccggaa tccagaacca gaaaagcgcc ccgccaacaa tccggaacat gctggtccag
     3061 tcgattgaac ggtacgtctg cacgagctgc cacggtccga aaagcctgcc ctcggcaatc
     3121 agcgtgtcgg tgaagcgatg gaagaccatc atttcaaagc cgaaaaggac gttcatcagc
     3181 gcggatttcc agaacgcgtg aagaagcggg aacccgagtc ccgggagcag tcccttcgca
     3241 agcagatcct cgacgccggc cgaaaacagc gggaagacca gggccagcat cgccccgatg
     3301 aaaccccaca ccagagcgcg ctgccacagt cttatgccgc ggagtctgaa gcggcccgtt
     3361 gcgatgcgtc cgcccagcag ttcgcccatt gtccccagaa ggacgaattt cgcaaaaccc
     3421 atcaggtacg ggtgtgcctt tgtcaggccc accaggacgc ccgcggcagg ctcgaccatc
     3481 agggccgcga tgattgcggc aagcagcgcc agccatgaaa ccgatatcag tatctgcatg
     3541 aaagtccctc tggacgtgcc tcgagttaga ggggaacata ccctgttcgg atgaatcatt
     3601 tcaagctggg aaagaaaggg gtggaagatg gcttcaaaga aatttccggc ggcgcttgcc
     3661 gggttgatcc ttctggttgg agtcggttgt ggtggtggcg agagcggcac cgacgtggtt
     3721 gacgacgctg gcgccgacat cgctggcgat atccaggccg acgttgccgg cgatatcccg
     3781 tccgacatcc cgtccgatgg aacggccgac gttgccggcg acgatggcgt ggcgccggac
     3841 attcattact ggcaggggcc gcagccgctt tcgcttgccg gtggaatata cgaccaggtt
     3901 ccgtcggtct tttcacggat aagccggttc gtcaccgagg tcgatgggcc cgagccggtc
     3961 gatccgtcgt tcctgggcgg agcgggggtc gggaatggtc ttgtcttcgc gatgttcggc
     4021 tatgggaatc ccctgaattc gatgcattcg tttgccggcc cgggttatga cctgggcgat
     4081 gaattcttcg gcgattattc cgtcggcctg gctgtcggcg gcgccaccga gaccgccgcc
     4141 ttcgaccggc aggtcgtcgc aatcacggcg gacgcgccgg tggtcgtggc cagggggacc
     4201 gtcgggcaga tcgaacttca cgttgtttcg ttcgccccgt ggtttgacgg tgccgtcacc
     4261 cccggaagcg attcggacgt tcttcgtcac tgcatcatgc gcgtggtcga agtgcgaaat
     4321 cccggcccgg ctgccccggt tgtcgtgcgg gtcctggccc acggcgacgt cgaatcgccg
     4381 caggccggcc ggatgcttga agaggatgac ggccgggtgc ttgtgaccgc cgcggctggc
     4441 gcgacagcgc aggtcgatgg cccggtcatg gcggttgacc ttggggaaat gggtgaaggc
     4501 gcaagccggt cgttcgtggt cacgcactgt gcgtttgccg ccggccagat tgttgagctt
     4561 ccggaattcg acgcggccgc actgcttgcc aggacggccg atgcgtacag gaagtgggaa
     4621 tcggaactgg ttgacgtgga aacgccggat ccgatggtca acgacttcat cgacgggatg
     4681 aagctgaccc tgaagctcca gacttcggat ggcggcgcga catgcccgat gtccgaatac
     4741 acgcggacat gggcgcggga caacatgggc ccggtgctgg cgatgctgtc gctgggcggg
     4801 tttgccgatg tcgaggctta cctggactac gtttattacg cgatcctgaa ggaaggcgac
     4861 ctgaagaaca gctacccggc gaacctggaa atcgactggg acggcgtggt cgcgcccgac
     4921 tgggattcga tgccgccgct tggtgaagga tcggtcggaa agaagcgggt gtctgcggaa
     4981 acgccgtcgt acatggtcct gatgtacgga cttcacaacc gtttcaccgg gaagaccgat
     5041 cgcatcgtcg aaaggcttgg acttctgcgc cgttgcctgt ttgcccaggg cttcagtgac
     5101 ggcgacatgc tgccgtttac cggggatgag acgttcaggg ccgccatgaa cgccgcactt
     5161 ggccttggcc tggaagaacc gcacgaagac acgaactggt ccggaaactc gtcggcgctg
     5221 tggcttggcg cggccaggga atacgcgcgc ttcatggaac aggccgggca ggatgccgaa
     5281 tccgcgatgg cccaggaaag gttcgaggtc gtcgaggcgg cgacgctggc ggcgtacacc
     5341 aggcccgaca aatgcttcgc ggcgcttgtc tcgatgtcgg acatggccat gtcgaaaccg
     5401 ttcgaggacg cgtcgctgca ggtgacgtgg tcgggctgga aggacgggga cgacccctat
     5461 gcacgcgatg cgttttcgtg cctttatgaa acggccagga cggttccggg cgagatatgc
     5521 tcgaagctgg acaccatgta cggcggggtg ttccgcgacg cgtgggacgg cgtatatacc
     5581 gggatgatgc cgggatatgc gctgcattcc atgacggtcc ttggacaccc ggatgccttc
     5641 gcggcgttca acatggtcag gaagtcgctc gatacgcttg gaaatgttca ggaatacttg
     5701 attcttgacg accattccgg cctgaccctg atgtacaacg acgacggcgt gacgacgcct
     5761 tcggactaca cggcgaagta tcgtccctgg gaaggcggca ttgttcttga cgcggtcctg
     5821 cagttcctgt tcggcttcga accggatgca aacacgcaac gtctggaact gcgtccgcac
     5881 gtgccgaacg ggtggccgca aatgagcgcg aaaggcctgc gggcggcttc gtcccggttc
     5941 gacgtttccg tttcttcatc ggggacggtc agtcgcgtga ccgttacttc gcgggccgat
     6001 gcggactgga tcgtttcgct gcggtgggac gacccggttc cgggcgttcg cggcccgacg
     6061 ttcacgcttg acggcggggt cgagccgttg cagggggtta ccaggatcga cacgccatgg
     6121 atgccgacct atcttcttcc cgacctggtt ctgaaggccg gcgaatcgct tcagattgtc
     6181 gtcacaggat gcggctgcgg cggctgatga acgtccgcgc cgccacaggt ggtcgcccgg
     6241 cgccgatagg ataaaaaaaa cggtgacata gcgatcttga accgccagtg tgatagtcgt
     6301 atgattacat aatctgtcgg tgcgcggtgt tgatatgaag ggttatcttc tggcggctgc
     6361 tgctttggtt ccgatcgtgc tgtccggttg cgccggcgaa tcgggcgttc aggacgtcgt
     6421 tgacgccgtt gataacgttg atgtcatggc ggatgccggc gacgtccctg gcgacgaaaa
     6481 tgcggtcggg ccggacatcg acgagcgcgt ggattcgtcg tccgacgtgt gcgttccctt
     6541 ctgtccggcc tgggccgaat gcggcgacga cggctgcggc ggttcgtgcg gcacgtgctg
     6601 gtgggaggag tggtgcgagg aaatgcactg cgtccacgtc gatcctccgc ccgattgcga
     6661 cggaatcgaa tgcggataca acgaagacgg gtatttgtgc ggggaatgcg acgaaggcat
     6721 ggaatgcatc gacgggcatt gcggcttcgt cgattatccc gattgcacca accgcgagtg
     6781 cggcgacgac ggggttggcg gttcgtgcgg acagtgcgac aaggggttta tctgtaatct
     6841 gtggggggaa tgcgagccga cctgcgagta cccgcaggat cttccggcgg gatggggaat
     6901 cgtcagcgtg gtcgatttcc tgacgattcc cgacacgacc gaagagaagg ccggctgttt
     6961 cgattacacc gatgatgggg tgccagacaa ccacctttcc gggttggcat cccagttcca
     7021 gacggttctt gacgatatgg gaacctggac cacaaacatg gcaatggagt tcaggccggt
     7081 taaggcaatc atcgccgatc ccggcatcac aatcgacgga atgcgcgtcc ggcatgacga
     7141 cgccgggcag ccgggaactt tcctggccga tccagtttca tggaatcccg acaactgtcg
     7201 tccgttcatc aggttcgatg acgtcgatgt cgaagaaggc gacatgtcgg ccggcacgca
     7261 ggaaattacg gttcatatca atgtgtcccc gtccttcatg cttccgataa ggctgattga
     7321 tgtccacatg tcggggattt tgcagcccat gggtgaaggc atcgaggtca cggaaggcgt
     7381 catatcggcc gtggccatgg gttcggacct gaaccaggcg gttaacctgc tttgggtcga
     7441 gtgcgacaag gaaccgcccc ctgaagacct ggtcgagacc tgccaggccc ttcagatggc
     7501 catggccccg cagcccagga ttgtcggccc gggattcctg ctgcagcttc accggaccgc
     7561 cgatggtacc tatctggaca gggattccga acatccgggg aacgcgatgg cgatgtgcat
     7621 gaagttttcc gcgaagcccg gaacgattac cggatatttc accccatagt tgtcacaccg
     7681 tcccggtttc gttcgatgta aaatcaagcg cccttcatcc agttcaaaag gacccggggg
     7741 aaagatggat cttgaagaat ttgtcggctc ggtcggttcc ggcgagacgg acgcggttat
     7801 gtcggtcatc gttgtttcca gtcggatcaa gaccttgctg gaaggcactt tccgtcgcgt
     7861 gtccgttgaa ggtgaagtga gcgggttcaa gccggcgatg tccgggcacg tctatttttc
     7921 gctggccgaa cgggatgcga agggttcgac ccagaagctg gactgcgtca tctaccggtt
     7981 cgccggggcc gggcgggcgc cgatattgaa ggatggcgac cgcgtcgtcg cgaccggccg
     8041 gataaccgcg tgggccggcg cgtccagata ccagatgtcg gtcgattcgg ttcgactgtc
     8101 cggttccgga aacctgttga ggcaactcga ggaactgcga tcccgcctga tgtcggaggg
     8161 gctcttcgac ccggcgcgcc gcaggccgat accgtcgttc ccgcgccgca tcggagtggt
     8221 cacgtcgatt cgcggtgcgg ccgtccggga tatcgcgcgg accattctct cccgctggcc
     8281 ggccgacata cttgtggtcg attcgcttgt ccaaggcgac ggcgcggcgg aaggcattgc
     8341 ccgcggcatc gaaatgctga accgggtgcc cgaagtcgat gtgatcatcg tcggtcgcgg
     8401 cggcggatcg atggaagacc tgtgggcctt caatctcgaa aaaacggtca gggcggtcgc
     8461 cggctcgcgc aagccggtga tttcggcggt cggccatgaa atcgatcacg tgctgaccga
     8521 tgacgtcgcc gatgcccgcg ccgcgacgcc gaccgcggcc ggccagatgg tcgttccttc
     8581 aagggatgaa atccaggttt caatcgacga cgcggcgtcc aggatggggc gtgccgtggc
     8641 gcggcgaatc gacgagtcgg cccagtcgat ggacgacgcc gccgaaatgc tggccgccgc
     8701 cgggcgccgg atgcttgatg cccgtgaaag gcttctggag tcgcgcaggg cccgccttgc
     8761 cgccggtcat ccggcccgac gcctgatgga caagtccagg catctggaaa cggccggcgt
     8821 gcgcctttca tcggccggga aaaggctgct tgaacggccc gcggcaaacc tggaaaggct
     8881 tcggctgcgg atcgagtcgt tcaatccggc gctgccgctt gatcgcggat tcgcgattgt
     8941 gacgacttcg ggcggcgctc tggttcgcgg gccttcccag ttgaagcagg gtgatgtcgt
     9001 gacggtaagg ttgaagaagg gcgcggtcga tgcacaggtt acatcggtca ggccggaaaa
     9061 cgggggatga ctcgatggaa gcgacggccg ggtgggcgat tgatttcatt cgaaaaattg
     9121 ttgccagggc gcccctgcgt ccgccggcca gcccgggcga acttgtcgcc cagcagatgg
     9181 tcgaggatga actgaaggcc atcgggcttc agacaagctg gcacaggttc cgttttcacc
     9241 gcagcattta tggcaatttc gcgcttcatt tcgggcttgc ggtcgcggcc accgcggtct
     9301 cgccgtggtg tccgatcggg gccgccgtgg tgcacgcgct ggtagccttc tcgtacgcga
     9361 tgcagtcaac caggcgccat tcggtgcttt ccaggcttta cttcaggcgg ccttcacaga
     9421 acctgcttgc cgtgatgccc gcggaaggcg gtcggccagc catcgctgcg cctggtcttc
     9481 atggggcacg ccgacgccgc tttcaacggg cttgtcttca atccggcatt cgttcgctcg
     9541 accatgaatt caaagggtcc gttcaggcgc tcgattgccc ttgcgacgtg ggcgaccgcg
     9601 gcgatggtgg cgtttgatct tctgcgggtg ttcgcggatc cgcttcccta tgtccggttc
     9661 gtggagtggg cggtcacggc gcccgcgatt atcgccttcc tgctgaattt cgacgtgtat
     9721 gtgcgcaacc gtcatgtcca gggcgccaac gacaacctga ccgccgcggc ggcaatgccg
     9781 gttctggcgg cgcggctcgg cccggaacgg ccgccggacg tggagcttgt gttcgcgttg
     9841 accgggtgcg aggaagccgg acttggcggc gcgatggccc ttcagcgcga agttcgaagc
     9901 aggtgggacc ggaagaacac cgttttcatc aacatggacg gcctttcaaa cggccagttg
     9961 cgttacatcg acccggaagg cgagcttttc ctgctgcgcc ccgaccggtg gctggtcgat
    10021 ctggttgacg aggttcgcgg gtcgcgcccg ggctttgcgc cgtttgccga cgtcgcccca
    10081 ttcgacatca cgattggcgg caccgacatg gccgcattcc agacggccgg atggcagggc
    10141 atctcgttcg tggcgattga tccggcgacc ggcagcccgc gtcattacca cacgacaagg
    10201 gacacggtcg ataaccttga ccttgaagaa tacgaggtgt cgctggactt catatccgaa
    10261 cttgcccgcc gcgtcatgaa gcggggttcg tcggccgtca aaaatgaaga aacagcagcc
    10321 gaccctcgtc cgagtcccat atcgtgactg ttctgctgtc gattcccgcc acgcaaacga
    10381 attcggtatc atggacgcgc ctgaggatgt cagtgtggaa gtggccgaag atccacgctt
    10441 ttggacgggt ctcaagtcca ttccacagtc gggtcagttc cgaatcccca caatcgcctt
    10501 caacgccggc gtcgaacccg gccgaggaaa tgaacgcgag caccccggga aggaagatcg
    10561 ggctgccgtg gattccaatt ccgatgccgg tcgggcacga gtgcgtgacg atcacgtccg
    10621 gcctctcgtt ccggaatcgt tcaagcgcgc tcgcaacgtc gtcctgttga atgtagttgg
    10681 aagatccgct tgcggagtcg aactcctgcg gtctgtcaac gtgcagggcc ccgccaagga
    10741 agccgaacct ggtgccgccg atgacggtca ccgaccctcg ttccatgtaa tgcaggtcaa
    10801 gccgtcgctt ccattcttcg acggttctgt tctcgcggca ggcggcaagc caggcgtggt
    10861 cttcatggtt gccgtcgatt gcgtacagcg gaaccggaaa gcgtggaaaa tcgtccggca
    10921 ggttgccaag gatttcgggg aagaatccga agtcgccgac ctgcacggcc gcaccgattc
    10981 gaagttcccg ccgcgcggcg tccagcatct cccaggtcga tcggatgtca ccgtggatgt
    11041 cgccgataaa aaggattctc atcgattgtc aggcagtcct tgcgaaatcg ttgcgcagcc
    11101 tggcctgcat caccacgata tcggcaaaaa tcttcttgat cgttgaaagt tcaagctggg
    11161 tcaggctttc aagatcaatg agattgtccg gttcctttcc gcccagaagc agtctggcct
    11221 ggtgcacaag gcgcaggcgc atcaggaagg aatatccctg gacgtattcc tcgcaactgc
    11281 tcttgtgaag aattcccgcc tcccgcaggc gtgtaaggcg cgccagcgtg ttgcactcgt
    11341 cgatcccgtt tttcagcgcg taaatccgcg cgaagttcac catcggggtc aaagcgttct
    11401 ttatgttgaa ggcggccggg ctttccgggg acgattcaag ctgcaggttg ccgaagaatc
    11461 cgatgggcgg cttgaactgc agagtggatt cggcaaggtt gaagaagaag gcgtgacggt
    11521 cggcgacaat ccggtggatg tggtcgcgaa gcagaaagac cagcatctca tcgccgaaga
    11581 cgtgcctgaa gtcgaagaac acgttgaagt ccagaagagc ctgcggcgtc tgggacgata
    11641 cgcattcgag aaagtaccgc tgccagtcgt gaaggggcct gcaccatttg ggattgccgg
    11701 ccatcacgtt cccgcggcac ttcaggtagc cgatctgatc caggtcttcg ttgacggccg
    11761 cgccaagttt caggaagtat gccgaaacct cttcttcgcg atcgtgaacc ggcgaataga
    11821 ttattccgtt gtcctgatcg gttctgaagg tctgttcgcg ccttgcctcg ctgccaagga
    11881 cgatgaatgc gaacggtgcc ggtggcggcc ccagcctttc aagcgccagc tcgataatcc
    11941 tgcggataga ggcatcggcg attccggaca tcatccttgt cgccgcgtca ggcgccgtcc
    12001 cgttttcaag cattgccgaa accagggcat tgaccttgcc gcgcacgccg gtcagttccc
    12061 cggcggaaga ggcgttctgg atgtttttta agatgatcgc cgccgggtgg cgctgggcca
    12121 gaaccacgtc gccaagcgag agtattcccg aaatccttcc gcgatcaccg cttatggcca
    12181 ggtgtcgtat gcccttttcc ttcatcagca gggcggcctc gaacataagg ctgccttcat
    12241 tgatgaccgc gagcggtgcc gtcatcaccg aagcgacgct gtcggccggc gagcgatgtt
    12301 gcaccaggac gcgctggcgc aagtccgtgt ccgtcacgat tccaaccggc tcgtccgcgt
    12361 tcgtccgcac catgatggca tccacgccgc gcgccgtcat catggcggcc gcctgctgaa
    12421 tcgacgtgtc gagcgtgcat tcgagcgtgt tgaggccggt cgcgccgatc gaggaattcg
    12481 aaaggagcag cgacgtctgg agatcggaca gaagtttcgt gacggccggc tgattcgcgc
    12541 ggccgcggcc gatatccttg aaagatatca ggcttccggc gcgtccgccc atggttacgg
    12601 gagtattgga gatgattacg tcgattgttt ttccatccct ggcaatcagt ttcgagtctt
    12661 taccgatgat ctcgtccatg gtcagcgagg tgaaggccgc cgcggagtgt ccggtcatgt
    12721 ccagcaacgc cctgttgcag aacacaggct tgccgtcgat ggcaagcatg atcccttcgg
    12781 tattcgattc gacaagcgtt cgatactttt cctcggagtc acgcagggcc gcctgggtca
    12841 gcgacctctg gcgctcgatg gccagagaac cccggatcat gtaaagaagg agaagcgcga
    12901 caagcagcga tatgccgagc gagatgttga ttaccttgcg ggtgaccttg tcgacttcgg
    12961 cccttacgtc ctcgatgtat atgccggttc cgatgaccca gtcccaaggc tcgaaacgtt
    13021 tgacgaagga aaccttggga acgatctttt tcgagtcgtc cttccactgc cacaggtagt
    13081 cgacgcgacc ttccccggca tcaagaacca cgcgggccat ctcgacgaaa agccgcttgc
    13141 ccctgggatc ggagtaatcc gagagatcct ggccttccag atccggacgg tatgggtgaa
    13201 gaatcattct gggatggctg tcggtgaccc agaaatagtc cttgccctcg tcgccgtacc
    13261 tgacggagcg aaggcgtgag agcgccgtct tctgggccgt ttccctgtcg attatcccat
    13321 cggcctgctg ttggtaaagc gcctcgattt cgctcagctc ggcccttgta agttcccgga
    13381 taagctcgtg tttcctttcg atcaggttgt ctttcatctc ggggatcacg atggcaaata
    13441 tcagcacgtt gaacaggatg acggccagga tgaccgggga cagtatgttg ataaggaagc
    13501 tcttccagtg ataacggacg tggcctggct tccgtttcaa ttggcgtccc ccggggatgc
    13561 gtttgtcgcg cactcccctt tcaaatagtt ggaatgtcct tgaaagtcaa gcggttgtcc
    13621 ctagaaatag atttggccct gcagggtgat catcgtcatg aacggcgagt ccaggacatc
    13681 cccctgtcgc ttgacggccg cgtccagctt gatgtttgcg ttgtgcttga cggcccacca
    13741 caccagtccg ccatggataa ccagcagatc cttgtccggc aggtcgctct tgaaccagtc
    13801 gattccgaat atcggcgaga acgtcttgta ccgataaccg aattcgccga acagcccgat
    13861 gccggaggct gcggatgcct tgccctgcca gtacctgtag aagtcggcca tcaggatgaa
    13921 ttcgtgatcc tttcccgcaa acgggacctc cgcgtacagg tcggccgata cgcccatgta
    13981 gtcgccaagt tttccatcct tcgcgatgat ggctgtgtcc tttccggcgg cagtcttttc
    14041 aaattcggca agcctgtcga tgccggcatc ctgctgatag tcgaagctga cgcccagcga
    14101 aacgatcggc ttgtccgcga aatggattcc tttcgcgaac atgtctgttt cggttcccag
    14161 gaagttgtaa cgcaagtggg cggtcaccct tggatagtcc ttcgggttgg taagaaccac
    14221 ggccttgtcg ttgatgtcct tctgaagaac gtcatttttc gacccgttca tgacgctgaa
    14281 gcggtactgc aattgttcct tgaagatata cccgcgtatc tgcgcgcccg cgtcccgcca
    14341 aaccttggtc gcttcggtcg ggtacttgat caggtccgag tggtattcca ggccgaacag
    14401 gctcacggcg ctctggtatc cgtgccggct gaagggggcg atgatcaggc cggcatccac
    14461 catgaactgg tcaaccacct tgaatgtcag gaaggcgtcc tggatgaaga aggtcggctt
    14521 gttccagtcg tttccctttc cccagttggg catgtcggta tcgatgaaga agctgatgta
    14581 cttgttgacc tggccgccaa gaagcagtcg aacccggcga aggaagaatt ccttgcccca
    14641 cgacttctga tccggggccc cttcctggat gagctgggcc tggaactgaa gaagcaggtt
    14701 gatgttgagg aacatgtcct tggactgttc cagcttgatt gcccttgccg gcatcgaaat
    14761 gcatgccagc gcaaggaaga tgacgaggga tgcctttctc atgtcgttcc tctccttatt
    14821 caggccaagt tcgtggctgg cttcttctgc gtcaacagag ataccacgac aagcgtcagg
    14881 aaacttgcag gaatcgatat aaggcccgga tttgtaaacg gtatcggcgc ggcggatgcc
    14941 tgaagcccgt acaagggata gatttccggg gaaagcagga tcaggccgac cgctgtcgtg
    15001 atgccgacga taatagatgc cacgattccc ttcgcggtag tcttcttcca gaacaggagc
    15061 atgatgattg ccggaaggtt tgccgaggcc gcgaccgcga aagccaggcc gaccaggaac
    15121 gaaacgttga tgccctggaa gatgatgccc agcacgatcg cgattatccc gaccacaacc
    15181 gccgcgatct ttccggcgcg aaccttggac ctgtcgtcaa gcttcatgcc caggaagcga
    15241 tccatcaagt catgcgcgac cgcccccgag gccgcgacaa tcaatcccga gacggttccc
    15301 aggacggtcg cgaaggcgat tgcggaaatt atcgcgaaaa gggccgtgcc gaaggatctg
    15361 gcaagcagcg ggccagacat gttgttgtcc gccgggttga ccacgctgtt gaccatggcg
    15421 ccaagaccca tgtacatcgt cagcacctag aacaggccga tgaccgcgat cgcgacgatg
    15481 gtgcttttcc tggccgccgc cggtgtcctg accgtgtagt acctgatgag gatgtgcgga
    15541 agagacgcgg tgccaaggaa aagggcaaga atcagtgaaa tgaagtcgaa tttctcaagc
    15601 ggagagcctt caatcttgaa tttctggccg ggccgcatca ccttgttgcc cgaagagagg
    15661 gtcgggtaat aggcggtgat tttttcgcca ccgtcttcaa atttcgtcga tttccaggtt
    15721 ttgaccaccg ttttatccga accgatcttc gaaaggaatt cgaacggacc gacagggcca
    15781 gtccgggctt catcgcccgt ctttccgtcg attgcttcca ggtttccgac aagtcgaagc
    15841 tggttctggc ccacggccgg ctcgccgttc accgtcttcg agccgtcggg aagaatcgac
    15901 gtccactggg tctcgtgaag ccttatcctg tcttcggcct gttccttcct gaaccatccg
    15961 actttctttt gatgcgccat cctgacgaac gtgccggcgg gtgttgtcgc gaccgaaaca
    16021 atcctgcttt cggcatccgg cggctgaagc acgccgtcga tttcggtggt ttcaacggtg
    16081 aggtacttgt ggaatggaac cgatccgccc tgatccggtt cggtggaaag gccgcggaca
    16141 agaagcgcca ccgtgatcac cagcgcgaag atgacaagaa gaccgccctt gaggaactgg
    16201 acgtaagtgg tcgaggtcat cccggcggtt gcgacgatgc cgatgacgat tgccccgacc
    16261 attatgacgc cggccgcgtg gggcaggccc agaagcggcg tgaccaggac gccggccccg
    16321 accatctgcg ggatcagata gaacaccgaa acgatcagcg tgctgatggc cgcggcaagc
    16381 tggattccct ttgagttgaa cttcgagtcc agggcatcgg tgaaggtgta ttttccaagt
    16441 cgcttcatcg gctcggccac gacgaacaag gcgacaatcc agccggccag gtatccgatc
    16501 gagtacagga atccatcata gccgacggtc gcaatcatgc cgcagatgcc aaggaaggat
    16561 gccgcggaaa gatagtcccc ggcaaacgcg accccgttga ccccccagtg gatgtttcca
    16621 ccggcagcgt agtatccgga cgcggatttt gccctgcgcg cgaagtaaaa ggacatcgaa
    16681 agcacaacgg cgacgaaggc aacgaaaatc attactgcca tggtcagtta cctccctcgt
    16741 ttgattccgc ggccaggcgc tcgcgtcgaa ggcacatcag gttgtagatg accgcgagga
    16801 tcagcgcgaa gaggatcagc gcgaccccat agacgacggc caggttcaat ccgaaaaaga
    16861 tcctggattc catcatcgcg gggtcaatca ggttgatcgc gacgaagccg gcatagacca
    16921 gcgcgtacaa gagaaacatc cagaccccca ggcgttgctt gtaactggcc gcgaaatcct
    16981 tttcgcattg gacggcaggt tcgtgcagca tggtttcccc cggaataaga gtaaaggtac
    17041 atgacaacaa acagccatcc cccggggacg gcctgaaata atgcggccgt tccgtccgct
    17101 tcggactgga tatacgggga atgaacgtga actttcgatt ctatccacgg aagaaaagcg
    17161 gagtcaatgg aaagttgtta caactgttac agcttgcttg tcggcgataa ttgttgctgt
    17221 gtcagctgtg aaattttgtg ttgcgcggac tgtcacaagg catgccccga catcagaaca
    17281 tggagtagtc gtcgtctttg atgcggcggg acatggtcga gcgcagggtg gtcacttcgc
    17341 ggcggaaatt gcctttcatg ccggcgacga gcggcaccac ctcgcgatcg acaaaatcaa
    17401 gcagttcctg aagatcctgc ctgcacatga acatgctctc ccccccgagc tttccaacca
    17461 tgggcagaag ctggatgtcg gttccaagac gcgcggccgt cctgatatgg tccggaattt
    17521 cgcggaagtt gcacttcatg atcacgaaag aaaggacgac ccttggaagg ggattgaatt
    17581 cgcgtcgcgc ccgcatcagt tcggtcacgt tgttgatgac cctgtcgtag gcgtcaacaa
    17641 gggtcacgtg gcggtaagtc tcgcggctgg ccgcgttgac cgagatgatg atggaaagaa
    17701 gacgggactt cttcatgtcg gatatcaggg atttgtccag caggatcccg ttggtcgtca
    17761 gcgcaattcc cccgtccctg aatctgtcgg tcttgaacgc cttcaacagg cgcctggttc
    17821 cgtcggcaat caggggctcc ccgccggtga tgttaagcac ccgaaggtac ggcatcatct
    17881 cgatgacctc gtcgaactgg cgttcggaca tcatcgtctt tgacggatca ggggcgtccc
    17941 ttacttcccc ggggaagcgg cacatgatgc acgcgatgtt gcagtgaagc gacggggcga
    18001 ttaccaggtt ctgcggcatg gactcaagga tctcggcgcc gtcgatgatt tccttcaggt
    18061 tggtgaccag gttcctgtgg aacacgtccg tcagcggatc ggcggtaagc cacgaatcgc
    18121 ccgggccgcc accgtggaag acgacgcagt cgggccggca gtgcaggtgc ggctggccga
    18181 tcttcatctg gtgccgcagc gactgcatca catcgctgtt ccaggcctgg gcaaggctga
    18241 cgtcgtgcca cgagacgcac tgatcgacgg tgccgtactg gaagcggtga gtggcgcagc
    18301 ttattgcgac cccgcggacg ctgtgcgttt caaggttggt ccacggattc atgcagatgg
    18361 ggtgacgacg ggcaagctcc tgtcgcaagg tgcgcagttc gttgcgcctg acccagatcg
    18421 ccgcggcgtc cagccacacc tgatcctggt cgccgccctg tccgccccgc agcggccgcg
    18481 gcggtgtcga aggttcaagg tgacgtccgg aaaaggtgca gatttcccgg gcgtcgcatg
    18541 cggcgcacgc ttcaaatgga gcctttgacc tggcgatatt gccgtccgac ggaattccgg
    18601 ccagatcccc ccatgcgagt gccgggacgt ttttcagcgg aaagatgcac ggtggcggcg
    18661 cctcgttgcg accgatctgg aagatgacgc cgttatcctg gcagaacttg attgtgcggg
    18721 ttaccgattc gatggcgatt gccggatccc acttattcgg cgtgccgtcc cgcctggatt
    18781 cggacacgaa gtcgagcgtg acgcgggccg gcatgcagag cctggacgcc ctcgcgaccg
    18841 tgtcgtcgat tgattccgcg ttggccttca tgacgggaat ctgaagcgtc acttccaggc
    18901 cgagttccct tgcgcccgaa agtatcatcg cggccatctt caggctgccg cggcctccgg
    18961 tcaggcctgc cagcatttca cgttcggccg acggaagtac aagctggatt ccggtcagtc
    19021 cggccgccgc aagatccttc agcgccgccg gatcggccgg cccgcagtgg acataggcgt
    19081 caatcttgtc cgcgccggcc tcggcaagtc gcgaaacggt cggggcgaac ctgcggtcaa
    19141 gaaggttgcc ggcaagcagg agataacgtg acgcctcggg gatttcgaag cggccggcca
    19201 cggcgtcatc ggggcagacc ccttcccgtc cctgaccgac ggtgcagaaa ctgcaaccca
    19261 ggctgcatcc cgcggcgatt cgtatcaggc ggctttcccc ggaaaggtcc atatcaatcc
    19321 gatgttcaaa ggggatccgt catccccgac cgcatccgca gcacggacag caggccctcc
    19381 agttgtcgcg ccgcatgcgg ccagacaagc atatacctgt caagcaggct tctgacaacg
    19441 cattccgcgt gtttcttcga cgcttcgtcc gtgaagaagc tttcgcccat ccgatccctt
    19501 tcgatgggca gcagtcgtac ttcggtcttc aggcgggcgg caagctcgat gaaggcgcca
    19561 agctcgccca ggttgctttt catgaccacg aaactggccg taacggtcgg cttgcgcggc
    19621 atcccgccgg cggccgcgac catcgccgcg acgttgccga tcaccttgtc gaacgtgcct
    19681 ctcgtcccgg tcacaagttc atgggtcgcc gcggtcgcgg cgttgatcga aacgatgatt
    19741 cgggccatcg atgtcggccg cagcctttca atcagcgccg gggtcagcag tgtaccgttt
    19801 gtggtaagtg tgtaactcgt atccggaagc cgctccggcg tgatcgattg aaggattttt
    19861 acgattcgtc ctgaagtaag cggttccccg cccgtcaacg ccaccgtcgc ggtggtcggc
    19921 agcagttcga caagctcgtc aagcagcgcc ggcggggtgt cgatgccggt cgcgccggcg
    19981 tcttcgtcgg tcaggtcgca catcctgcag cgctggttgc acttcaggct gggcgagatc
    20041 gtgatgttgc gcggccgcga cttgacgctg gccgaaccct cgatgaattc cgaaagcccc
    20101 agggcaaggt tggaatcaaa cacctcggtc cggggcagcc tgaccggagg cagatccgac
    20161 cagctttcgg caaacctggg gcagtgccgg ttgcacgttg cctggcgtcc gccgtcgacc
    20221 atcagtcgac gcatcgaggt catcctgtcg ccctgaaacg atttcaggat tccgtcggtc
    20281 agccagccgt cgcaggtgcc gtccggcgtg cccaggattt cgcgtcttgg ccaggaacct
    20341 tcgcacgggg caacccgtcc ggttctgtca tgaacctcga tcgaatccca cggcgccatg
    20401 caccggtcaa tcgtggccgc ccgcgcaaga agtgaatcga gcctttccct gcgggagaaa
    20461 agcgtcgtcg tcctgcatcg ggacaagccg gaagcgacgg gcacgcctgg ttcgggggag
    20521 cggtcgggat cgacccacgc gatcggatcc ggcgccttgc ccgcgaacgc gtgcattccc
    20581 atgttgcacg acttccggat gccgcacgaa gcgcatgagg cggcgacggc agccccgcgt
    20641 tcacgcagaa ggccgggaga tccgtcaaga cccgggtaat gttcgacttt tgcgtcctcg
    20701 atatcgatga cgcacggggc cggtccctcg aagccaccga tcgtcaggga aatcccggcg
    20761 gcatgggcca tggccgcgat ttcccgtatc ggttcggacg ccagcctgaa gtcccaggcg
    20821 ccagccgaag ctggtccgtg agtcgccctg aagggcaggg ccacctttcg gatggtcggg
    20881 caaaggccct tgacccgcct gaaggtatcg gtgattccag gcaggctttc ctgcgtcacc
    20941 ggcagttccg ccgtgacgtc gaggtgaagc gatgcggccg ccttcagcag cagcgcgatt
    21001 gattccagcc tggcgcgggc gccgcagagt tttgcaagcg tggccctgtc ggatgcggga
    21061 atcacgacgt gaaggcccgt cagcccgctt tcggccatcg tcttaagcag tgtcacatcg
    21121 ttgcaggccg ggtggacgta aagaaaaacc tcttcggatc cgagttcgcg cagtgacttg
    21181 attatcggga caacgtcttc gtgaatcagg tcgccggcca tgatcgtcgc gcggtggcaa
    21241 acaggcagat ccctgatttc gagcgacgcc ggcgccgggc cgatgcgtcc ttccgacatg
    21301 gtacacgaca ggcagccgca accgcatcca cggccgacaa agacaatccc gctttcttcc
    21361 cggttcatcg gaaaacggcc ccggcaggtt ccagccagcc ggaattttac cagatagccg
    21421 gttcgttgtt tgaaggtatg tttgcgagcc atggttcccg ggcggcgccg gcctagaacg
    21481 agatggcgag caggtagtcc gtcctggcgt cgtcacgctg ggcaggcgag attttgacga
    21541 agacggttcc cgagtcgtcg aatccaccgc tgcataccgg atcgaatgat atttgttcgg
    21601 cgattcccca gcgccgcctg gattcgaagc atcgggcccc ttcgattgaa gaaacggttt
    21661 caaacacgtc gcccgaagga tccagatccg gttccgtaag ttttcccctg tcgcacctgt
    21721 aacagaccat caggttcagg tcggcgtcct cggacatccc gtccagcctt acatcgaccg
    21781 caaggtattc gccccaggtg tcctcgaccg tggcctcgta ccagtcgcga tcctccggtt
    21841 ccagtatgtt cgcgatgacg tcccagtcag tggagttgtc gccatcggtg aattcgccca
    21901 ggtacgcggc ctgtcccggg tagttgtccg gttcatacac gtccttgtcg aacgtgacgg
    21961 tcgcgcagcc gccatcgtag cagacctggc tggcggggca tcgcgcgcag agacagtttc
    22021 caatggcgcc gcactcgatg tcgtcgcaca ccttcgggca gtccggcatg cacagcccgt
    22081 tttcgcactt cattctgtcc atgcatccgc cgttcatcga cccgcaggtg cattccccga
    22141 tattgccgca ttcccggccg gccgtggagc acacgcactg gcagtcggtg acgatgcgtc
    22201 cggcggcgca gcgctggcag acgccggcgg tgccgcagtc gcagccgtca accgtcccgc
    22261 attcgatgcc ctggcagctt gttctgcacg atacgcagcg gccggccgaa gtgcagccgt
    22321 aacctttctg gcacgggcag tcttcggggc aatccaggca gttttcaaaa tccttcaggc
    22381 attccccgtc gccgcagtcg ccgggaaccg cgcactgccc ccagttgccg cacaccagtc
    22441 cgtcggtgca tcttccgcaa tcacaacctt cgaattcgcc gcagttcctg tgatcctgcg
    22501 cgcaggcgac actgcaatcc atgcagcttc cgtcgatgca ggcgtgggac gcggggcagg
    22561 cgcaatcgac gggcgaagtg gcgcagtctt caagtttttc gcagacgccg tcttcgtctt
    22621 ccgggatgga aaggcaatca ccgtcccggc agtacatgtt cgccccgcaa ccgcagtcgg
    22681 ccgggcaggt cgtgcagttt tcaaaggaat ccgtgcagat atggtcgccg cacaggtact
    22741 gggttcctgc acaggttcca tcggcgcggc atgaatccat gacggttgtc gcatttgaat
    22801 cgtcacacgg atggccgacc tgctggcgtc ttgtcgagca gtatccaagg tcgcagatcg
    22861 gaacgtcgca catcctggaa tgtagcgcct gatcaccgca gtcgtcgttg gtggtgcacg
    22921 acttcggcgt ctggatgtcc ggctttccgg cgttccccgc gtccgatacc gggatgtccc
    22981 ggtcggcgcc gtattcttcc cattcgccga cggacggcac gccgcaggcc aggagcaatg
    23041 cggcgacaaa cggtgcggcc gatccggcgg atcttatggt ggcgacaacc cgcggccgat
    23101 gtgcgcggcg cacgtaccgt gaagacgtcg tgactgacat gttgaacccc ctgtggtgat
    23161 aagagaatcc gggccggtga ttcgccggga caggggcttt atcgatgaaa tgctgtctgc
    23221 tgtttcagtc gcaggcgagg cgggctccgc aaacggtcac aagggggcag tccgcgcagt
    23281 tcgggccggc cgagccccgg caggtgcccg aaattcccag gtgcgacagg gcgaagtcgt
    23341 acctgaccgg gtcttcctga tcgagttcgg caagctgcct ggtaagggcg atagcgtcct
    23401 tcatgcgcgg tgcgttgttc tggccggcat ccccaaggcc gagcaggccg gaaatcctga
    23461 acacgtgaac gtccagtgga aaaagaagat ccgaggggcg cagcccgttc cagatgccca
    23521 ggtcgatccc gtcgcgcctg accatccaac gcaggagcat gcacagccgc ttcatcggcg
    23581 agtcggggtt tccgaccgga accaggaatc cggggtcgcc gtcgccggat gatcgcagcg
    23641 cgtccgcaag tgaaagaagg ccattgaccg ttcccccggc cgcaaaaccg gacatgaccg
    23701 aaggttcaag cgatccgtcg cgccgcagaa gcgcggcggt gccggacagc aacgcctcga
    23761 cgttaggggg tgtcgcgaag cggtaaatca cgccgtcggc cacttctcga aacagggcgc
    23821 ggttaccttc cgccagtgcc tgtccggggc tttcgccaag acgtgaaagc accttgtcga
    23881 taacgggcat gaaagcggca acgcgcccga aggcgaatgc ggacgcgatg acggccgcgg
    23941 cctcgcggtc gatcggatcc cggtattgcc ttggaaacga cagtgggtcg cacgcaagcg
    24001 attcctcggt gtggaacctg tcgtaaacgg catcaaggaa aggcttcagt cgggttacat
    24061 ccggccgcat tcgatgttct ccctggtctt gaacagggta atcgggaagt ccttgaagtg
    24121 cttcagcttc ttttcgttga acgtccttgc cggctggaac cgtgacttga acggctccgc
    24181 ggaagtcatc ttgtcaaacg ggaagtacga acctgaggcc gatacgacga aggcgacctt
    24241 gtccgcacag acctggtcga tgaagcctgc gtccgagagc atgcccgaac tgaaacgttt
    24301 gccgttcgtg tcggcctcgt ccgccgaaat ccgcctgccg gcgtgaagcg ccacaagagg
    24361 ggccagcgtg gaagccccgg tcagcgtctg ttcggggatg gtgttctgcc tgacgaagtc
    24421 ggcgatctca tcgacgctgt caaacgccag agacttgttc catatgtagt gccggtaata
    24481 cggcgtgatg gcaccacgtt cgcggctgtc ggcgaagaat agtgccttcg tcaggccggc
    24541 cggtcccttc aggattgcag ggtcacgcca ttcgtacgcg accttctgtc ccttcaactt
    24601 cgactcctcc ggccacagtg cagcgttcag gtgggccgac cacggaacgg caagccacgc
    24661 ggccgcaaga acggcaaggg gaaatgccag gctggatgcg ccggacgccc gcttcatgcg
    24721 ggaaacggcc tgatcgacgg catagccgac ggccgggcac atgaaggaca gcatcggaac
    24781 cagatagaag tcatagactt cgttgacggc tgcccactgt aaaaggaaaa gggctgtggc
    24841 cgcaacgctg gaaagggcga tgaagtgcgg cttgaaggca tcggttcgaa agccgcggat
    24901 ggcttcccgc acggccatcc atgtggccgg cagcagaagc aggaacatgg caccgtggta
    24961 atagagggtc ttcaggaagg tcgggccctt caggaaggcc gtaaggttgg aaatcaggat
    25021 cacggccatc ctgaaggggt tgggacttga aaagagcgac tgtcttccct cggcgctgac
    25081 agccttcctg gtgtggtaaa ggaagacccc gtcgaagaac gacttgccgc cggaaattcc
    25141 gaaaatcagc agcattagcg caaaaatgcc aatgaatccg ataaggtagc ggacggcggc
    25201 cttgcggtcg gcgacaagga ttgcgattcc caccgccagg acgcccgcca gcgcgtacat
    25261 gccggttccg atcgccagtc ccgccaggac gcccgcaacc actggaaaac gtctgaagcc
    25321 ggcccaggcg gccgcccaca ggaacatcgt catgatgttt tcgccgttca tgtcggttga
    25381 gcccatcaga atctggtacg ttgaaaagtg ggcaaacagg gcaaccaggg ccattggccg
    25441 ggaaaccggc cgtagcgtca ggtacatgaa aagcccggca atcgtctggg ccagaaccgg
    25501 aatcagcttt gcggcgccga tggagtatcc cgcaatcttg aacacggcgg ccgggacaag
    25561 caggtgcacc ggggggtggc tgaagaagaa gtcccggtaa ggcaccgcgc cttccgcaac
    25621 gcggtttgcc aggtaaaaat agatgttgtc atcggtgccg gaaggatgca ggccgaccag
    25681 cttcaaaagg aaccacgtgc caaggatgac ggcaagcccg gccgcctcca gggcaagcgc
    25741 ggctcgccgg ccgttgaatc gcgccagtcg cgggatgccc gacagcattg aaagggacag
    25801 gatggcggca agcggtattc gcgtcaccgc cggaagcacg tgaaaagggc cgtagggcag
    25861 cacggaaagt atcggcatga ccgctgccag aaccccgacc atggctgcgg aaaccggcgc
    25921 cggctccgtc cgggtcgcgg tcgccgagcc ttgcctgagc cttcttgaag ccttgcccat
    25981 cgttttcccc tgaaagaagg tcgttgcgcg actgcttagt gtaacgcaac acggtgcctg
    26041 ttcgacatgg aagtcaactg tcgggcaatt gcgccccgca tcgcgaccgg cggaggaaca
    26101 cttcgatgga ttcacggaag gccgaaggtc tgaggatcga ggaactggtg gccggggaac
    26161 ttgtccggcg cggatatcgg ataatagcca ggaactatcg caagccctgg ggtgaaatcg
    26221 atatcgtggc cataagcggg cgccagatcg tcgtgtgcga ggtcaggtca agaagtcgtg
    26281 gagttgcggc ggatgcggcc gaaagcgtcg gaatcgaaaa gaggaagaag atacggatga
    26341 cgaccgagtc gtaccttcag gagtgcccat tcctgttcga tgaagtcagg ttctttgtcg
    26401 cggccgtggt gatgtctgcg ggccgacctg aaatcgagat tatcgaggat gccttctgaa
    26461 ctcggagaaa gagttcagcg ggaacggagc atcgccgcaa tcggcttgaa cccgcgtcga
    26521 tggatagggc atggcccaag gcgatccagc gcggcgcggt gcgcggcggt tccgtagccc
    26581 ttgtgggaag aaaaggcgta gcctggatag acaagatcca tttcttccat cagccgatcc
    26641 ctggaaacct tcgccagtat cgatgcggcc gcgatggcat aggagaggtg atccccgcga
    26701 acccaggccc tctggggcat tgcgagtccc ggaatgatgt tcgtgccgtc gacaaccacg
    26761 atggatggca tcaccctggc gcagacctgc tcgaccgcgg ccttcatcgc cttcagcgtc
    26821 gcgccgagga tgttcaacga ctcgatgtcc tggcacgaag actgggcgac ggcccaggcc
    26881 acagcctgtt cctttatcgg cgaatacagc gcgtcgcgat cggcgtgcga aagtgtcttg
    26941 gaatcgtcaa gatcgggaag atcaaatcgt tcgggcagga ttacggcagc cgcgaaaacc
    27001 gggccggccc agcagccccg gccggcctca tcgacgccgg caattgacgg gaaaccgcct
    27061 tttcgggccc agtcttcaat gaatcccggc ccggacggca aatcattcag aagctgagtc
    27121 tgtatcatcg gtcttgctga ccgcgccgac cagccacgtc ttccgctcgc tgatcttcgc
    27181 cgccttgccc ttcagggcgc gcaggtagtt cagcttggcg cggcggacac ggccgcgtgc
    27241 gacgacttcc agcctggaaa ccatcggact gtgaaccggg aaaatccttt cgacgccgat
    27301 tccgtaagag atcttgcgga cggtgaaggt ttcgtggatt ccgttgccct tgcgaccgat
    27361 gacatagcct tcgaacatct ggaggcgttc cttgtcgcct tcacggacgc gaacgaaaac
    27421 cttcacggtg tcgccgggat gaaaatcggg aagatccccc ctgcggtgga tctgctcgag
    27481 catctggatt gtctgatgca tataaagcct cgtaatcgtg cacgatttat caaaaacaac
    27541 ccttcggcca aacccgggat cgccttgatc gccgatgttc agaaccccaa gggcacacta
    27601 gtatatgctg acacccatca agcggtcaag agtgattgct gcggcagccc ttacagacag
    27661 gtgtcggtat tccgtcggcc cggagatggg ctccagcctg atgtccgact tctcgatgat
    27721 gctgttggcg agtccccacc cggtgccgaa gacgatcagc agcccgggga tcttcccgga
    27781 ttcgatcgtt ttgcgaagat cggcgaaggt cgtggcatcg cgcatcgggt tggcgctggt
    27841 caccgcgacc tggggcctga tcccgaacgt caactggaag tcttcaatgg ccgactggag
    27901 gtcatgaacc gtccggacaa gctcaagcgc cggcccgcga gggtgcccgg cctttccgcc
    27961 cgccccttcc gaccagtggc cggcgactcg gtcaaccatt tcgcgttggg ccaggattgg
    28021 tgtcaccaga tagtaccggt tcagtccata ggtacggcac gctcgcgcga tgtcgtggat
    28081 atcgaccgat gtcaccgagg tggcgacaac ctccccgatc ctgtcatata ccgggtggtg
    28141 caccagtgca gcggcaatct gacagacagg tttattcatc gacaggtctt ccccagccgg
    28201 tcgggatccc cggatttgcg gtcaaaggcg cctgcccgtt gtcatgcgcg gattcccggg
    28261 gggcttccgg cgctgtttcc ggttccaggc gatcgacaag atcgggccgg ttcgccctgg
    28321 tcttcgcaag cgcgcattcg cgtcggaatc ttgcgatttt cgcgtgattg ccggaaaaga
    28381 gcacgtccgg aaccagatgt ccctcgaaat cccggggccg cgtgtattga gggcattcga
    28441 gcatcgagtc gttgtgcgac tcgtcctgca ggctttcaac gtttccaagc acgccgggga
    28501 tcagcctggc cgtcgcgtcc atgacggtca atgccggaag ctcgccgccg ctgagcacgt
    28561 aatctccgac gcagacttcc atgtcgcaga agtgctcggc gattcgctca tcgacgcctt
    28621 cgtaatgacc gcacacaagg atcaggccgc cggaatggct tgcgaaccgt gtcgccatcg
    28681 cctggtcaaa aagtcttcca gaagcggaca tcagcacgac cagtgcatcg ggcatcagtt
    28741 cctttgcggc gcggattgcc ccggcaacag gttccggttt catgaccatg cccggtccgc
    28801 cgccgaacgg gatgtcgtcg gcggtgcggt gcctgtcggt agtgaaatcg cggatgtcga
    28861 ttgcatcgat gtcaatcagg ccggccgtgg cagcgcggcc gataattgaa tgggcagccg
    28921 cttcacgaac catctgtgga aacagtgaaa ggatcctgaa tctgaacgga accggcgatg
    28981 aaggcagaaa atctgtcatt ccatctccag cgcgtccggt tcgattatca ccgcgtcgcg
    29041 atccatccgc cgcacgaagc cttcaaccac cggaatcatc cattcctggc cggaatcgga
    29101 tgtgatttcg ataacgtccg tcgcaaggga aagaacctgg gatacctttc cgacgacctg
    29161 tccgtccggg aacatcaccg gcagtccgat tgcatcccga tagtagaatt catcgggcgc
    29221 cagttcggga aggtcatcgc ggtcgcagca cacgatggcg ccgcggagct tttcggcggc
    29281 attccggtcg gtgcagccgg ccagtccgac gataaggaac tttcccttgt cgcgtatgga
    29341 ttcgaccgtc atgaccgtcg attttccgt
//
LOCUS       DAOPPA010000002        24572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_2576_length_24572_cov_4.307950, whole genome shotgun sequence.
ACCESSION   DAOPPA010000002 DAOPPA010000000
VERSION     DAOPPA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 24572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24572
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_24572_cov_4.307950"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PKH54_00125"
     CDS             complement(<1..1233)
                     /locus_tag="PKH54_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOC98319.1"
                     /translation="MQTGSCRTFGAALSAFVAATMMLPAAFASTSVSMRADSREIAWD
                     GTVTIEIVMEGEFDEARGPDMPDFKVVGRSSGSSVSIVNGTVSRQQRVTLALAPLREG
                     ELAIGKIELVREGRVVATSGALKIKVSGGGGQSPSPAGSPPVQPMPEDEPEPGAAQAA
                     DEPEAATASPPGSVGRGQVPAEMAGRQAFILPRVPDRPVYAGEPIYVEYALYTRSDVP
                     LSGLALNSVPELKGFIIDNPSQDSESVRRVAIGGRRFEERIIWRAAVTAIDPGKKTFD
                     PISIDLVTGGMFSSRRHKLASEPLDVTFAEVPLQGRPADFIPGTIGQYIVNSSLDKPI
                     VKIGESAILTVEITGSGNLRALKPPRIVEPDGVRVDRVPSSDLDELRIDRGGISGRKV
                     FQFLLTPEREGTFELGRID"
     gene            complement(1248..3839)
                     /locus_tag="PKH54_00130"
     CDS             complement(1248..3839)
                     /locus_tag="PKH54_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC98320.1"
                     /translation="MFQFESIRWGNPDLWWLLLLPAMLVPAWVWQELSFRKILGSFPG
                     TRLVPSMVHGSGTGRRLATFLCAVVALELLGVAAMRPRYGLKDYAVKGIGVDIAIVLD
                     ASRSMKVSDVAPDRLGKSIIEISRLLRASGGNRVALVPFAGISFIQSPLTVDHEVIRQ
                     YLNDLRVTDIPVPGTALGRALNTAGRALGIDQAGFRGSAHKAIVVFTDGENHEGEPAE
                     VARNLAQKGVRIFTVGVGTPEGKPIPRLNDKGVVTGLTRADDGVSPVLSKLDEALLKE
                     VAEVTGGKYFQLSGLAAGASVSEQLMKEIDSLQKAEYMSAVERLLEDRFQYPLAAAIV
                     FMLLPFLWAGVRRRPGPARVRAAGAAMMVAVLALSMPASAEPFEHPVLARLFNTSHGG
                     VDDATELIQENRHSEALTLLKELATRIPPSPALDLDLALAAFGSGDFGAAVEYAEGAI
                     RKNDALPEGSPIRLSPASLHHAKGTMLAARARAMSVEKKPPREVRKVYRQAVDSLVAA
                     LLLDPENPATRRNLEIVATAAYPPCSSLDDAREPNNTRADAAFLTPDPSTLEAAETLL
                     LCPADTDWFKLPVNQGETLFVSVQKPPEVAAAPGGDPSAPAQQQKPEPADVDLSLEGD
                     DGTLIADGVKQARWTAPDDAGSTMLMKVTGPQVEDGVDYFLSARIVPPCPSGDDQMEP
                     NDSREATKAIQEGEMALRSCPLNDDWFQFVEQQGQAKEVRLTSSNSDDPVDLQVFSAD
                     GASMDVRTDIGPDGTVRSVLLPKAEQEAPFTILVSSGAGQAFYTLSIQDPEGGQNDRQ
                     NDDQQNNEQKPDEQEGKDRQQEQKSQGSRTMRELIDAIDSNDRNLEAQEAARNNPMGD
                     SSPDKDW"
     gene            complement(3856..4962)
                     /locus_tag="PKH54_00135"
     CDS             complement(3856..4962)
                     /locus_tag="PKH54_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC98321.1"
                     /translation="MRFESPWMFLLLVPVAGMVAFGLYSLLRKPAHLDIPTVRHATAA
                     GSGFRGRMAHLPAVLTGIAAVLAVVALARPQVQDHEELVGEGADFVIALDMSASMNAV
                     DMPAEDILSNQAAGHEPPNRFESARQILKKFISSRDMDRVGLVIFASKAYVKFPLTLD
                     RDAMTRILDGLVLDDRMRSQDGNCVNGCTITGEQTAIGDALARAFKRIEDSKTKSRNI
                     ILITDGDNNAGMAAPTEIADFIALESGANPVRVFTFLVGGGKDTWMPAFNGFSGDLLK
                     AGNGMQVYEPVKDQAPVKPDLLREIAEKTGGRFFAAPSESDFRKEFSDLEKTEFSAPA
                     LQAFREAYAWPLAAALVLYVAGLALGTTIWRRWP"
     gene            complement(4959..6020)
                     /locus_tag="PKH54_00140"
     CDS             complement(4959..6020)
                     /locus_tag="PKH54_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98322.1"
                     /translation="MKTSFLPLLAVLLTVSAGAQRAVAQTDVTNEDFVAGAGAEADAP
                     PGGPRGGEASVTVTMDPTNEPVQIGRPFHVEIRFGYPVGTRIYFPDAPDTKPLTLVSV
                     KSEDRTVLGTSTSESHKLILLAIKTGEAILKPFEVPVVLPDGHAASVMTPELRINVSG
                     SLGNEEAPELAPPGETRPVIVRNDLLIWILVALAAAAVASLTAIMVYRRWRAWADAHR
                     PPPPPVPPHERARARLAEIEAMGLVEQRDFKTLALLVSEVIREFLGAVLRFPGTDSTT
                     WETLESVRRASGGNAVGKMSLMELEDFLALCDLIKFAKFEPSVGDAQALVRRSHELVD
                     MVMCGPTDQAGRDGDGGAS"
     gene            complement(6035..6967)
                     /locus_tag="PKH54_00145"
     CDS             complement(6035..6967)
                     /locus_tag="PKH54_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC98323.1"
                     /translation="MKTEDTDSVKSLLARVRRIEITAQRLVNEQLSGRYHSVFKGRGM
                     SFDEVRPYTPGDEVRLIDWNVTARTGDAFIKRFVEERELTVIIMADASLSMNFGLSSC
                     SVTQRQKTTEIHDTKRELAAQIAAAIVLSAQMNNDRVGLITFGETVTNFIPPGKGRRH
                     ALRVIREVLAMEAAGSRTDLAGALAYLGRILKRRTAVFILSDFEVPDFERELRVAARR
                     HDIHGLMVRDHFEDNLFEGGIVTLRDLETGLVRPFFTGAGFLQRYRSSVQAGIREASG
                     QFQKMKLPFSRFSTSEPYDRALSGHFARLKGKAL"
     gene            complement(6985..7974)
                     /locus_tag="PKH54_00150"
     CDS             complement(6985..7974)
                     /locus_tag="PKH54_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC98324.1"
                     /translation="MEQDVRILTEMVRKESAFVDRLTAEVGRVIVGQKELIDRILIAL
                     LCRGHVLIEGVPGLAKTLTVKTVADALDATFSRVQFTPDLLPADLTGTQIYNQQTGAF
                     SVKKGPIFAGIVLADEINRAPAKVQSALLEAMQERQVTISDKAFPLPEPFLVLATQNP
                     IEQEGTYPLPEAQIDRFMLMVRVSYPTRDEELQIMDRMALSVPTKVDKVISLDDLQRA
                     RSMMSEIYMDQKVKQYIVDLVFATRRPDEAGIPDLADYIRYGASPRATIALSEAARAH
                     AFLRHRGYVIPEDVKSIGLDVLRHRLIPSYEAEAENMTAEDILNRIFDSVQVP"
     gene            8237..10642
                     /locus_tag="PKH54_00155"
     CDS             8237..10642
                     /locus_tag="PKH54_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC98325.1"
                     /translation="MNINDDNARGTCVSPLLCDDFQNVPAVAGRSREGLVRVGVFVAA
                     ALLFFIGAGSFGLWDCWETHYGETARYMFENHDTLSPWWGYKDKIGNESPTGEWFFSK
                     PVLIMYGDIIGMHLVGFGDWAIRLPWALLGSLVVLFAYVHFSLIFNRRTGLLAAGMLM
                     TAPLFFFLSRQAITDLPYAGTMAIGMLFMSSAMFAPRYPVSNRGLVVRLVAVLALFLL
                     TAVPQFVNTWLDLFPEGNYQRLTGLNRIGFMFQKSGWIHSLMYFAASGALLAWVLVPM
                     WRQYRAGILFSDEGKDLWIRRFMLWTAYMFFGYATLGKGLLGFCLPGAMLFLYFLLTG
                     EWKMLRRFDLGRGLLFFALVMLPWYVGMFCRHGMGFYNRFLVHDHFNRLGAGVHQIDT
                     GTFEHFMRWLSVGTFPWIAFIPLVIWGITRLRLKDPDPRSRMKLLMYVWAFFAYVLFT
                     LSATKYHHYIFPAVFPTVFLVALLVKDFFEDRGWIGRFVAIVGLGIMLSVGLYIRSDM
                     QVFRQMFTYKYDRPLPTRLPNDPDAPVADGTSMTWKESTFYEFTSPVVQKIIQAPALQ
                     YKTFLTAFLVVASICFLLMIFGGRFRKGGLAALWGISLALAWWGLNFYMPTLSPAWSQ
                     RYIFEDYFKRCELMENSEDVNSTYTPALKKIGLGFIPDSLGYFNKRVCREDVVAWLIT
                     WRGETYFTHSEIKPLMKANQLKPYLKDLNRGKRFFALTQPNRSSSLKSSLDKETKSLR
                     EAGAEGFDSIRSWKVETVHWESDYFSLVMCTPITDDSTETDQPEEKPAVPPEEPETAG
                     LGM"
     gene            complement(10649..11824)
                     /locus_tag="PKH54_00160"
     CDS             complement(10649..11824)
                     /locus_tag="PKH54_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOC98326.1"
                     /translation="MEMNETRSLLVTIRDRWLASAGPDGLVPGPFLDARDISDLAAST
                     GWAGMHLARAIRLAFEPFDDESIDRLFVPTRAPRFRGRTPHRADLLAILAGRIPALAA
                     KVLFHGLAGGMRVILKPSSAETVFPALLVNSIMLLERDAPVSVAPGRGPALDAMIRSA
                     TACVAYGSDMTVEAIAAARRGRPTFPGRHMESFETVFADAIESNDEARAVAEKIAFDT
                     AIYDQSGCLSPTVVFVQEGGGVSARAFAGMLLEALSGSQLAMGPMEIDDLAAVRIFIQ
                     EAAIGRIKDPVFSREDVIPPAVVLCDRVRPSPGLRTLQVVPFDSPVPDLSRHMPDLAD
                     RIQGMACAGSDGEVGWLLRKNPRFRANHICAPGELQNPPALWRENGIVLAHELLGIQ"
     gene            complement(11878..12942)
                     /locus_tag="PKH54_00165"
     CDS             complement(11878..12942)
                     /locus_tag="PKH54_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98327.1"
                     /translation="MIDKSLVDAVMTMIGDSVRGHSGIDDEGFESLAVEIAAAQGRLR
                     RTSPLSANLRGLDKIEPLPETAFKMMTVAQFPPSEAVAEFRTSGTSSENRGRLPVLDM
                     EIYKASALAGFELFCMHDDPPTRFMSLIPPASARPDSSLSWMATFLVEKFDDGLGGFV
                     AGTQGLDPDGMLSILSRSVSDGSRVFLLGTSLDFLSLFEFLVMNATPIPLPAGSRLMH
                     TGGPKASGRSITPRQLAADAGRLLGIAPQDVIEEFGMTELFSQAYDSPRISGVPGRLI
                     PVPWMRTRVIDPRTMKDVPDGERGLLVHYDLACCHTCVAIMAADTATRSGNGFCDIRR
                     AAGAAARGCSRNAAPRPGGA"
     gene            complement(12939..14288)
                     /locus_tag="PKH54_00170"
     CDS             complement(12939..14288)
                     /locus_tag="PKH54_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOC98328.1"
                     /translation="MAGTFGQSRRAVRGARVKSALDRLEAGRGENPHPAVADRFPMVI
                     GSARGARVKDADGNRYIDMTSFFGVALAGHRNPAVMSAVRVAQGHLVSSMGDVYPDPG
                     RAGLLQLLAGMMPAEGYRGVLSQNGADAVETALKFAAAATGRPGIVSFEGAYHGLSAG
                     ALEATWRPAFRKPFEAMLSGRAVFAPWPEPDGSNLELVLSTVERLASSPIINRFGADI
                     GRPGALIVEPIQGRGGVRVPPPSFLSRLASICDRNGIVLITDEIYCGTWRTGTFLASE
                     PEAVVPDIVCLGKALGGGFPISVAMMRPAVADAVLPDHGEAVHTSTFMGWPVSCAAAI
                     ATIAAIKRMEPLEAATRIERAVTAAARSWAGKFDFVRGVAGRGAMMGVSLAGTRGMSA
                     SEVTRKVVSEALSRGVLLLAEGSDSDVLALMPPLVIGDDDLGTALRIIGDSLAKVAE"
     gene            complement(14315..15811)
                     /locus_tag="PKH54_00175"
     CDS             complement(14315..15811)
                     /locus_tag="PKH54_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98329.1"
                     /translation="MTRKVPASARQTAAMTVTTFVVLTCCGRAYGQWPRFDAGAQSEP
                     DVEIESLLPGQAPRPRPATQPPRPGLVPEAIDQRCGKFGWSLAEPVMIPVAFPLVLDL
                     TLYGELEPDAVRMADAMAEQLAATGIFMVNNRDIAPAGGAITWASPVAFDYLGWRSAG
                     NWMVVTATIAPPSGDTTDKEKLTVSLSAWLTEEGNSLQVSSSTATVRKDELISFCGRF
                     VDAVGQCVSGIPSAASSRIAYSKRTPPHGVKEIWVTRVGSAEQTRISSDGKLAMLPAW
                     APGGSLAWTGYRNDNPDLYVDGVRFGNRPGMNTGIAWAPDGGRAAVTWADEGASDIYL
                     VNPVSGEIISRLTSWPGIETSPSWSPDSKQIAFVSDRGGSPGIFVMNADGSGARPLPL
                     DGDYNTGPDWSPDGKTILYQSRGAGSRFSIHAFDVLSGTSKRLSTDNFNNEEPSWSPD
                     GRFIAYTSTRDGRKKLFIMNRDGSGVRQVFKDGDEYFTPAWERVIPKK"
     gene            complement(15819..16724)
                     /locus_tag="PKH54_00180"
     CDS             complement(15819..16724)
                     /locus_tag="PKH54_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HOC98330.1"
                     /translation="MRISDYIAQALGVSDRRPVQAGLAVTALVHVAIVVMLATAGGNA
                     ALPPKPGMPGTGSMCDGIRCAEPPFMSDRRGADSMDTMDAGIIEASIIPKLGLAKPVP
                     GQLPRFLKYEQAEKIEESINISKDNQKTEQPLKKDVRPKKAQTDSRRKKTDLGDILGD
                     APDDDDPRARAVALDRIVGHAEGSTSGTGTEAREGSIYAAKVGEAIRAQFTVPPFLKP
                     AQLKVLRVRIRITRIDAAGQILDFDVLSRSGEESFNNAALFAIRRFSRKDGGNVYLPS
                     PDSKTLELVNKRGIVVDLDGSLFRK"
     gene            complement(16753..17340)
                     /locus_tag="PKH54_00185"
     CDS             complement(16753..17340)
                     /locus_tag="PKH54_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC98331.1"
                     /translation="MGMSGSSGSNRGMMAEINVTPLVDVMLVLLIIFMISSSIETIQV
                     QQEKQRIEKTSETANLNQKVPVNLPRTDAEPVNLSEEQKLVLSITPDVKFFVGETMIL
                     DCLSIAPAIKATVTGDSRRIWSVKDDAAFGPCLDQIVAKLGTNAKLQNDKELYLRADR
                     SLPYGLTLKVMARIRSSGVGKFGLVSEAEGGNEGG"
     gene            complement(17372..18079)
                     /locus_tag="PKH54_00190"
     CDS             complement(17372..18079)
                     /locus_tag="PKH54_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC98332.1"
                     /translation="MEPGAAESFGINVFQQVLASRGMVLMVLLLLIAMSLVCWFIIGV
                     KFWSFRKIRRQTEEFIKAFWHSSNLEEVREASRQLSDSPVARMFSAAYTEMKRNQEGQ
                     GTTGPVGQGSDYGFENIERTLRRAVQEQTTRLEKLTPFLATTGSAAPFIGLFGTVWGI
                     MNAFAYISPDRPILETVTPHIAQALVATAIGLLAAIPAVMAFNYFNSKIRVFVVEMDN
                     FSIDFLNILKRHFFRDR"
     gene            18300..19526
                     /gene="ribA"
                     /locus_tag="PKH54_00195"
     CDS             18300..19526
                     /gene="ribA"
                     /locus_tag="PKH54_00195"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOC98333.1"
                     /translation="MVESSSIPHPVTAAVADAVDSLRLGGMVIIVDDQSRCNEGDLVL
                     AADMVTPEAVNRMTKIAGGLVCVAMTHDMVDRIGIPMMASNSSAHFGSAFTVSVDSRL
                     GTTTGISAFDRARTIEDLCAEDASMQDFVVPGHVFPLRALPGGVLNRAGQTEAAVDLV
                     RMAGRKPAAVISQVLGEDGSIAGLKQLLELGRANGIPVVAVADVIAWRMQKDLLVRAV
                     GSTMVDTEWGRFKSVVYEDTIRRAAHVAMVMGEPGPGPTLVRVHSECLTGDVFHSLRC
                     DCGLQLDASMRRIASEGGVLLYLRQEGRGIGIGNKVKAYALQDRGLDTVDANTSLGFA
                     PDLRDYGTGAQILAHLGLRKLRLLTNNPRKMVGLSGFGLEVVERVQIELSPRSDRDRH
                     YLETKKSRMGHLLEKV"
     gene            19657..21435
                     /locus_tag="PKH54_00200"
     CDS             19657..21435
                     /locus_tag="PKH54_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC98334.1"
                     /translation="MLAELRKHSKSVIIYVLFGIIIVVFVFTFNMSSRRRDSSVGYGR
                     QYENAELVVVGDSRIDVVDLEMGLRLTTDAPLPGKERTAESERQERAYRQYRFHDFPG
                     DPSMAIFGQDPMGVASIKSRKVKEDLVETWLVSEEARKNGLVATPDEVRERILKRFRD
                     PTTGEFKRSYYENWVRYQIRTTMPRFEDFVAREIEREKMISLVTSAVAVPEREAKYVA
                     GLRKNARSYEFLEISPALLGAALAGNQKELAAWQKANQKAVREYFDANKSEFAQEAGL
                     DFHVLKYSDQARATEVFEGLKDLKGEELRDAMTALAPTSEDKVTNEIGGRSQAALSVG
                     AIASLYGQEVADAAQKLTELELSPVVKSAGGYFLLMLDARRPQVEPVFEEAAEVIGRR
                     LFGEQKAAAEMEALSTAAIAALNAAPERPLAEVAAELNAKYAPHSPVRVGETGEVRKL
                     PPSVMGLFMWEPDTYRGIGKGKSITTALEGLTNESRLLQQPLKPEGGTGVFVVRLAGE
                     VKVQEPTREEIDLVASELALFKRVAVWRDWFDALRAGAASSGELVEKELLAKMIADEE
                     KALEQAVSGTAPADSANAGGEETQNN"
     gene            21457..22335
                     /locus_tag="PKH54_00205"
     CDS             21457..22335
                     /locus_tag="PKH54_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOC98335.1"
                     /translation="MSSFRIRSNDPIAPSVFRMEVEAPDIAKYRRAGQFVIVHPSGGR
                     ERIPLTIADADPVAGTITLVYQVVGRTTAEMALLPAGGDLGDVAGPLGKPTHVENFGT
                     VVCVGGGIGVAPSHPIAQAMKSAGNRVISILGARTADLIIMEREMRLASDDVICVTDD
                     GSYGRKGLVTDALKDLLDDPSAPINLVVAIGPPVMMKAVSELTRPYGVKTFVSLNTVM
                     IDGTGMCGGCRVTVGGKTKFVCVDGPEFDGHGVDFDGMMRRQRMYSVDEKASYQAFLK
                     DHGCRLEERIRELKRG"
     gene            22328..23767
                     /gene="gltA"
                     /locus_tag="PKH54_00210"
     CDS             22328..23767
                     /gene="gltA"
                     /locus_tag="PKH54_00210"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOC98336.1"
                     /translation="MAEKLTPRQRLAIPPVPMREQDPGERRRNIREVPFGYSAEEAML
                     EASRCLLCRNKPCIDGCPVAVDIPGFIELVSLGLFNQAAELIRKTNCLPAVCGRVCPQ
                     EEQCQAVCTVAKSHKDPAMSVQIGKLERFVADWDREHPVEGAVASDVTPTGRKVAIIG
                     SGPGGLTAAGDLADAGHEVTVFEALHRTGGVLVYGIPEFRLPKDIVEFEVDGLVRRGV
                     RIVTNVAIGKSKSIDDLFAEGYDAVFIATGAGLPWFKNIPGEDSLGVYSANEYLTRAN
                     LMDAYRFGDGSDTPIMRGRTVVTFGGGNVAMDAARTALRLGADESIIVYRRSLAEMPA
                     RLEEIHHAQEEGVRFELLQDAVEVVADDAGWVTGVRCQKMALGEPDRSGRPRPVPIEG
                     SEFVMPCSVVICAIGNGANPILTEATPAIETGRWGNIVADPVTGRTSMKGVWAGGDIV
                     RGGATVILAMGDGRRAAASINEYLQTGVW"
     gene            23818..>24572
                     /locus_tag="PKH54_00215"
     CDS             23818..>24572
                     /locus_tag="PKH54_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98337.1"
                     /translation="MLELLCGIVCLFAGVAGMIWVIRDGRRAADLMSRAAVVLQVAVV
                     LLLLVDWGHSWVEMSGIDIVTGRFAWLALPHVLALVSLVGMLVYGPDFRGRAGVLATV
                     QTGSLIGVMLLAEQEIDLSFLAPVPLVSLYLSAIVAFAFFGSSLVVLPRPDSFWYRFV
                     FKPRENLLASIEALKECGFDVTRPETVFECGSASGWLEGTMVHISTAPSLYPLRYGLR
                     IRIAVSRTWHGRRLPGFAENEKWLPGLGWIEYSG"
BASE COUNT         4570 a         7426 c         7555 g         5021 t
ORIGIN      
        1 gtcgatcctt ccaagttcaa aagttccttc cctttcaggg gtcagcagga actggaagac
       61 cttgcgtccc gatatgccgc cgcggtcgat gcgcagttcg tcgaggtcgg acgatggaac
      121 ccgatcgacc ctgacgccgt cgggctcgac aatccttggc ggcttcaaag ccctgagatt
      181 gccggatccc gtgatttcaa ccgtcaggat cgccgattcc ccgatcttaa cgatcggctt
      241 gtccagcgaa gagttgacga tgtactgacc gatggtgccg gggatgaaat cggccggccg
      301 cccctgaagg gggacttccg cgaaggtgac atcaagcggc tcgctggcca gtttatgcct
      361 tctggacgaa aacatgcctc cggtgaccag gtcgattgaa atcggatcga aagtcttttt
      421 ccccgggtcg attgccgtga cggcagccct ccatatgatt ctttcctcaa agcggcgtcc
      481 gccgatagcg acccgtcgaa ccgactccga atcctgggat gggttgtcga tgatgaagcc
      541 tttcagttcg ggaacgctgt ttaaggccag accggacaga ggtacgtccg accttgtgta
      601 gagggcgtat tcgacataaa tcggctcacc cgcatagacc ggtcgatccg gaacccttgg
      661 caggatgaat gcctgcctgc ccgccatctc cgccggcacc tgcccgcgcc cgactgaacc
      721 tggcggcgag gccgtcgccg cttccggctc atcggcggcc tgcgcggccc cgggttccgg
      781 ctcgtcttcc ggcattggct ggaccggcgg cgaaccggca ggcgacgggg attgaccccc
      841 accgccggac accttgattt tcagggcacc cgaggtcgcg accacgcgcc cttcgcgcac
      901 aagttcgatc tttccgattg cgagttcgcc ttcccgcagc ggtgcaagcg caagggtgac
      961 cctctgctgc cgactgaccg ttccgttgac gatcgagacc gaacttccgg aggagcgacc
     1021 gaccaccttg aagtcaggca tgtccggtcc gcgcgcctcg tcgaattcgc cttccatcac
     1081 gatttcgatc gtcacggttc cgtcccaggc gatttccctg ctgtcggcgc gcatcgatac
     1141 cgaggtcgat gcaaaagccg ccggcagcat catggttgcc gccacgaagg ccgacaacgc
     1201 ggcgccgaag gtacggcatg atccggtctg cacgcctttc atctgcctta ccagtccttg
     1261 tccggactcg agtcgcccat cgggttgttc cttgcggcct cctgggcctc gaggttccgg
     1321 tcgttgctgt caatcgcgtc aatgagctcg cgcattgtac gcgatccctg tgatttttgt
     1381 tcctgctgcc gatccttccc ttcctgttca tcgggcttct gctcgttatt ctgctggtcg
     1441 tcattctgcc gatcgttctg tccgccttca ggatcctgga ttgaaagggt atagaaggcc
     1501 tgtccggcgc cggatgaaac caggatcgtg aacggggcct cctgctccgc ctttggaaga
     1561 aggacggatc gcaccgttcc atccggcccg atgtctgtcc tgacgtccat cgaggccccg
     1621 tccgcgctga atacctgaag atcgaccgga tcgtcggagt ttgacgatgt cagacgaacc
     1681 tcctttgcct gtccctgttg ctcgacaaac tggaaccagt cgtcgttgag aggacaggag
     1741 cgaagggcca tctcgccttc ctggatggcc ttcgttgctt ccctggaatc gttcggttcc
     1801 atctggtcgt ccccggacgg acaaggtgga acgatcctgg ccgacaggaa gtaatcgaca
     1861 ccgtcctcaa cctgcgggcc ggtcaccttc atcaacatgg tcgagcccgc gtcgtccggc
     1921 gcggtccacc ttgcctgttt gaccccatca gcaatcaggg tgccgtcatc tccttcaagg
     1981 gacagatcga cgtcggccgg ttccggtttc tgctgttgcg ccggggcaga cggatctcca
     2041 cctggcgccg cggcgacctc gggcggcttc tgaaccgaaa cgaaaagggt ttccccctgg
     2101 ttgactggaa gtttgaacca gtcggtgtcg gccgggcaca ggagcagagt ctcggcggcc
     2161 tccagggtac tcggatccgg ggtcaggaag gcggcgtcag cgcgggtgtt gttgggttcc
     2221 cttgcatcgt ccaggctgga gcagggtggg tacgccgccg tcgccactat ttcaaggttg
     2281 cgcctggtgg ccgggttttc cggatcgagc agcagggcgg ccacaaggga atcgaccgcc
     2341 tgcctgtaaa cctttcggac ttcccgcggc ggcttctttt cgacggacat tgcgcgggct
     2401 ctggctgcca gcatggttcc cttcgcatga tgaagcgaag cgggactgag cctgatcggg
     2461 gaaccctcgg gcagggcatc gttcttcctg atggcgcctt cggcgtactc cacggccgcg
     2521 ccgaagtcgc cggatccgaa tgcggcaagc gccaggtcaa ggtcaagcgc cggcgacggc
     2581 gggattctgg tggccagttc cttaagaagc gtcagggcct cggagtgcct gttttcctga
     2641 atcagttccg tggcgtcgtc aacgcctccg tggctggtgt tgaacagtct ggcaagaacc
     2701 gggtgttcga aaggttccgc actggcgggc atcgacagcg ccagcaccgc caccatcatg
     2761 gcggcacccg ccgcgcggac gcgggcaggg ccgggccttc gtcggacgcc ggcccagagg
     2821 aacggcagca gcatgaacac gatggcggcc gcgagggggt attggaacct gtcctccagc
     2881 agtcgttcga ccgccgacat gtattcggcc ttctgaagcg aatcgatttc cttcatgagc
     2941 tgttcggaaa cgcttgcgcc ggcggccaga ccggaaagct ggaagtactt tccccctgtc
     3001 acctcggcaa cttccttcag cagcgcctcg tcaagttttg aaagaaccgg ggaaaccccg
     3061 tcgtcggccc gggtcaaccc tgtcacgaca cccttgtcgt tcaggcgcgg gattggcttg
     3121 ccttccggtg ttccgacacc gacggtaaag attcggacgc ctttctgcgc caggttcctt
     3181 gccacttcag ccggttcacc ttcgtggttc tcgccgtcgg tgaacacgac gatggccttg
     3241 tgcgccgatc ccctgaagcc tgcctgatcg atgccaagcg ccctgccggc ggtgttaagg
     3301 gcccttccca gcgcggttcc cgggacgggg atgtccgtaa cgcgcagatc gttcaggtac
     3361 tgccgtatca cctcgtgatc caccgtaagc gggctctgga tgaacgaaat tccggcgaac
     3421 gggacaaggg cgacacggtt gccgccggaa gcacgcagga gtctggaaat ttcgatgatc
     3481 gacttgccga gcctgtccgg ggcgacgtcg gagaccttca tggatcggct ggcatcgaga
     3541 acgattgcta tatcgacacc gatccccttg accgcgtagt ccttcaatcc gtatcggggc
     3601 ctcatggccg cgacgccaag cagttcaagg gcgacgacgg cgcaaaggaa tgtcgccagg
     3661 cgcctgccgg ttccggaacc gtgaaccatt gacgggacga gcctggtgcc cgggaaggaa
     3721 ccgagaatct tcctgaacga aagctcctgc cagacccacg cgggaaccag catggctggc
     3781 agcaggagca gccaccacag atccgggtta ccccatctga ttgattcaaa ctggaacact
     3841 tccattctcc tgccgtcacg gccagcggcg ccagatggtc gttccgagcg ccagtccggc
     3901 cacgtaaagc acaagcgcgg cagccagcgg ccacgcgtac gcttccctga atgcctgcag
     3961 cgcgggggcc gaaaattccg tcttttccag gtcggaaaat tccttgcgga agtcgctttc
     4021 cgaaggagcg gcaaagaaac gtccgccagt cttttcggcg atttcacgca gcagatccgg
     4081 cttcacgggc gcctgatcct tgacaggctc atagacctgc atgccgttgc cggctttcag
     4141 gagatcgccg gaaaaaccgt tgaaagccgg catccatgtg tccttgccgc caccgaccag
     4201 gaacgtgaag acccgaaccg ggttcgcgcc cgactcgagc gcgataaaat ccgcgatttc
     4261 agtcggggcg gccatcccgg cgttgttgtc accgtccgtt atcaggatga tgttgcggct
     4321 tttcgtcttc gagtcctcga ttctcttgaa agcccgggcc agggcgtcgc cgatggccgt
     4381 ctgctcgccg gttatcgtgc atccgttgac gcagtttcca tcctggctgc gcatccggtc
     4441 gtcaagaacc agcccgtcaa ggatcctggt catcgcgtcc ctgtccagtg tcagtggaaa
     4501 cttcacgtag gccttcgagg cgaaaatgac caggccgaca cgatccatgt ctctggacga
     4561 gatgaatttt ttcagaatct gtctcgcgga ctcgaacctg ttcggtggtt catgcccggc
     4621 cgcctggttg gaaaggatgt cttcggccgg catatcgacg gcgttcatcg atgccgacat
     4681 gtcgagcgcg atgacgaaat cggcgccttc tccgaccagt tcctcgtgat cctgtacctg
     4741 gggccttgcg agcgccacga ccgccagaac tgccgcgatt ccggtcagaa cggccggaag
     4801 gtgggccatc cgtccgcgga atccggatcc ggcggccgtc gcgtgccgca cggtcggaat
     4861 gtccaggtgc gccggtttcc gaagcagcga gtacagtccg aaagcgacca ttccggccac
     4921 tggaaccaga agaaggaaca tccacgggga ttcgaatctc atgacgcacc cccgtcaccg
     4981 tcacggccgg cctggtcagt cggcccgcac atgaccatgt caaccagttc gtggcttctg
     5041 cggacaaggg cctgtgcgtc cccgaccgac ggctcgaact tcgcgaactt gatgaggtcg
     5101 cacagggcca ggaagtcctc gagttccatc aggctcatct ttccaaccgc gttcccgccg
     5161 gaggcacgcc gcaccgactc aagcgtctcc caggtcgtgg aatcggttcc cgggaagcgg
     5221 agcacggcac caaggaattc gcggatgacc tcggatacca gcagtgccag ggtcttgaag
     5281 tcgcgttgtt cgaccagtcc cattgcctcg atttcggcaa gcctggccct tgcgcgttca
     5341 tggggcggca ctggcggggg cggcggtctg tgggcgtctg cccaggcgcg ccatctgcga
     5401 taaaccatta tagccgtcag tgaagccacg gccgcggccg ccagagcgac cagaatccat
     5461 atcagcaggt cgttgcgcac gatcaccggg cgcgtttcac ctggcggtgc cagttcgggg
     5521 gcctcctcgt tgccaagcga accggatacg ttgatgcgaa gttccggagt catgaccgat
     5581 gccgcgtgac cgtcggggag caccaccgga acctcgaacg gcttcaggat cgcctcaccc
     5641 gtcttgatcg caagcaggat aagtttgtgg ctttccgagg tcgaggttcc gagtaccgtg
     5701 cgatcttccg acttcactga aacaagggtc aggggcttcg tgtccggcgc gtcagggaaa
     5761 tagattctgg tgccgacggg gtatccgaag cggatttcca catgaaacgg cctgccgatc
     5821 tgaaccggct cgttcgtggg atccatcgtt accgtcacgg aggcttcccc gccgcgggga
     5881 cctccgggcg gggcatcggc ttcggcccca gccccggcca caaagtcctc gttcgtgacg
     5941 tccgtctggg cgaccgcccg ctgcgcgcct gcggacacgg tcagcagcac ggcaagaagt
     6001 ggcagaaatg aagttttcat atccgcaatc ccggttacag ggccttgccc ttaagccttg
     6061 caaaatgacc ggagagcgcc ctgtcgtaag gctccgatgt cgaaaagcgc gaaaacggga
     6121 gcttcatctt ctggaactgg cctgaagcct ccctgatgcc ggcctgaacg cttgaccggt
     6181 acctttgaag gaatccggcc ccggtgaaga acggtctgac cagaccggtc tccagatccc
     6241 tcagggtgac gatgccgcct tcgaaaaggt tgtcctcgaa gtgatcccgc accatcaatc
     6301 cgtggatgtc gtggcgcctg gcggccacgc gaagctccct ttcgaaatcg ggaacctcga
     6361 agtcggaaag gatgaatacg gcggtgcgcc gcttcagtat tcttccaaga tatgcaagcg
     6421 cgccggccag gtcggtcctg ctgccggcgg cctccatcgc aaggacttcg cggatgaccc
     6481 tgagcgcgtg acggcggccc ttgccgggcg ggatgaagtt ggtaacggtt tcgccgaacg
     6541 ttatcagacc gacccggtcg ttgttcatct gggccgaaag cacgatcgcg gccgcaatct
     6601 gggctgcaag ttcccttttc gtgtcgtgga tttcagtcgt cttctgtctc tgggtgacag
     6661 agcagctcga cagcccgaaa ttcatggaaa gggacgcatc cgccatgata atgacggtca
     6721 gttcgcgctc ctcgacgaaa cgtttgatga acgcatctcc cgtgcgggcg gtcacgttcc
     6781 agtcgatcag ccggacttcg tcgcccggag tgtacggccg tacctcgtcg aaactcatgc
     6841 cgcggccctt gaagaccgag tggtaccgcc ccgagagctg ctcgttgacc agcctctggg
     6901 ccgtgatctc gatcctgcga accctggcga gcagtgactt gacgctgtca gtgtcttccg
     6961 tcttcatatt cattccgcct ggtgctacgg aacctgaacc gaatcaaaga tcctgttcag
     7021 gatgtcctcg gcagtcatgt tctccgcctc ggcctcgtac gacggaatca gtctgtgacg
     7081 aagcacgtcc aggccgatgg acttcacgtc ctcgggaatc acgtagcccc ggtgccgcag
     7141 gaacgcgtgg gccctggccg cctcggaaag cgcgatggtc gccctgggcg acgccccgta
     7201 cctgatgtag tcggcaagat ccggaatccc ggcttcgtcc ggccgccgcg tcgcgaatac
     7261 aaggtcaacg atgtactgct tgaccttctg atccatgtag atttccgaca tcatcgatct
     7321 tgcgcgctgc aggtcgtcaa gcgaaatgac cttgtcaacc ttcgtcggca ccgaaagggc
     7381 catccgatcc atgatctgga gttcttcgtc gcgcgtcggg tacgacaccc ggaccatgag
     7441 catgaaccga tcgatctgtg cctcgggaag gggataggtt ccttcctgct cgatcgggtt
     7501 ctgggtcgca aggaccagga atggttccgg cagcgggaaa gctttgtcgc tgatggtgac
     7561 ctgccgttcc tgcatcgctt ccagaagcgc gctctgaact tttgccggag cacggttgat
     7621 ttcgtccgca agcacgattc ccgcgaaaat cggccccttc ttgaccgaga aggctccggt
     7681 ctgctggttg tagatctggg tgccggtcag atccgccggc aggagatccg gcgtgaactg
     7741 cacgcgcgaa aatgtggcgt ccagcgcatc ggcgaccgtc ttgaccgtca acgtcttcgc
     7801 cagtcccggc acgccttcga taaggacgtg accgcggcac agcagggcaa tcagaatgcg
     7861 gtcgatcagt tccttctgtc cgacaatcac gcgcccgact tcggcagtga gcctgtcaac
     7921 gaacgcgctt tccttccgaa ccatctctgt cagtatgcga acgtcctgtt ccatttatga
     7981 gtcctcccgg gcaaaaaggg cccgaaaaca gcctttccgg caccgcgcgg cgccatcgac
     8041 ccccgatgaa caacgcccgg gttcgagcta ttcccgatga cccggattcg cacgcgcacg
     8101 cgacgggcag gataagtgtg catcgcggtc ggacaggtca aggggcaaag gggccgatac
     8161 gtaaaggacg ttccttgcaa atctcgccca aaccgttaaa atccgggcga tttgactctt
     8221 tggtggtgct tggccagtga atatcaacga cgacaatgca cgcggaacat gcgtttcacc
     8281 ccttctgtgc gatgacttcc agaacgtccc ggcggtcgcc ggccgatcgc gagaggggct
     8341 ggtcagggtt ggggttttcg tcgccgcggc cctgcttttt ttcattggcg ccggttcgtt
     8401 cggcctgtgg gattgctggg aaacgcatta cggcgaaacc gcaaggtaca tgttcgaaaa
     8461 tcacgacacc ctgtcgccgt ggtggggtta caaggacaag atcggcaacg aatccccgac
     8521 cggtgaatgg ttcttttcga agcctgtcct catcatgtac ggtgacatca tcggcatgca
     8581 cctggtcggg ttcggcgact gggcgatccg cttgccgtgg gcccttctcg gcagtcttgt
     8641 ggtgcttttc gcgtacgtcc atttttcatt gatattcaac cgtcggacgg ggctgctcgc
     8701 cgcgggaatg cttatgacgg ccccgctttt cttcttcctt tcccgacagg ccatcaccga
     8761 cctgccgtat gccgggacga tggcgatagg catgctcttc atgtcctcgg cgatgttcgc
     8821 gccgcgctac ccggtatcca atcgtgggct cgtggttcgg cttgtcgcgg ttctggccct
     8881 ctttctgctg acggccgttc cccagttcgt gaacacctgg ctggatctct tccccgaggg
     8941 gaactatcag cgccttaccg gcttgaaccg cattggtttc atgttccaga aaagcggctg
     9001 gattcattca ctgatgtatt tcgccgcttc cggcgcgctg cttgcctggg tgcttgtccc
     9061 gatgtggcgc cagtacaggg ccgggatcct cttttcggac gaaggcaagg acctgtggat
     9121 ccgtcgcttc atgctctgga ccgcctacat gttcttcggg tacgcgaccc ttggaaaggg
     9181 actgctcggg ttctgcctgc ccggagccat gctcttcctt tacttcctgc tcaccggcga
     9241 atggaagatg ctgaggcggt tcgaccttgg tcgcggcctg ctctttttcg cgcttgtcat
     9301 gcttccatgg tacgtcggca tgttctgccg ccatggaatg gggttttaca accgcttcct
     9361 ggttcatgat catttcaaca ggcttggcgc cggggttcac cagatcgata ccggcacatt
     9421 cgagcacttc atgcgctggc tttcggtcgg aaccttcccg tggattgcgt tcattccgct
     9481 ggtgatctgg ggtatcacaa ggctcaggct gaaggatccc gatccgcgct ccagaatgaa
     9541 gctcctgatg tacgtctggg ccttcttcgc atacgtgctt ttcacgctgt cggccaccaa
     9601 gtatcaccac tatatcttcc cggccgtctt cccgaccgtt ttcctggtcg cgctgctggt
     9661 gaaggatttc ttcgaggatc gcggctggat cggaaggttc gtggcgatcg tcggactcgg
     9721 gatcatgctg tcggtcgggc tctacatccg cagcgacatg caggtcttca ggcagatgtt
     9781 cacctacaag tacgacaggc cgctgccgac caggctgccg aacgatccgg acgcgccggt
     9841 cgcggacggc acctcgatga cctggaagga atcgacgttc tatgaattca ccagccccgt
     9901 ggtgcagaaa atcatccagg cgcctgccct tcagtacaag acgttcctta cggctttcct
     9961 tgtcgtggcg tcaatctgct tcctgctgat gatctttggc gggcgcttca ggaagggggg
    10021 gctcgcggcg ctctggggaa tatctttggc ccttgcatgg tggggcctga atttctacat
    10081 gccgaccctg tcgccggcat ggtcccagcg atacatcttc gaggattact tcaagcggtg
    10141 cgagctgatg gaaaacagcg aggacgtcaa cagtacgtac acgcccgcgc tgaagaagat
    10201 cgggcttggg ttcatccccg attcgctggg gtatttcaac aagcgcgtgt gcagggaaga
    10261 tgtggtcgcg tggcttatca catggcgcgg cgagacttac ttcacgcaca gcgagatcaa
    10321 gccgttgatg aaggccaacc agttgaagcc gtatctgaag gatctgaacc gcggaaagag
    10381 gtttttcgcc ctgacccagc cgaatcgatc ttccagcctg aagtcgtcgc tggacaagga
    10441 gacgaagagc cttcgcgagg cgggcgcgga aggtttcgat tcgatcagat cctggaaggt
    10501 ggagacggtt cactgggaaa gcgactattt cagcctggtc atgtgcaccc cgattaccga
    10561 cgattccacc gagaccgacc agccggagga aaagccagcg gttccgcccg aggagcccga
    10621 gaccgccggt ctgggaatgt gactttcctt actgaattcc aagcagttcg tgggcaagca
    10681 cgatgccgtt ttcgcgccac agggccggag ggttctgcaa ttctcccggc gcgcagatgt
    10741 ggtttgccct gaatcgtgga ttcttgcgca gcagccatcc gacttccccg tcggaaccgg
    10801 cgcaggccat tccctggatg cgatcggcca gatccggcat gtgacgggaa aggtcgggca
    10861 ccggcgagtc gaacggaaca acctgcaggg tgcgcagtcc cggcgacggc ctgacccggt
    10921 cgcaaagaac caccgccggc ggaatcacgt cctcacgtga aaataccgga tccttgatcc
    10981 ggccgattgc agcctcctga atgaagatcc tgaccgccgc aaggtcgtcg atttccatcg
    11041 gacccatggc cagctgggat ccggacaggg cttcgaggag cattcccgca aacgctcttg
    11101 cgctgacgcc gccgccttcc tgaacgaaca cgacggttgg ggacagacac ccggattgat
    11161 cataaatcgc cgtgtcaaat gcgatctttt ccgccacggc ccttgcttcg tcattcgatt
    11221 cgatggcatc ggcaaaaacc gtttcgaacg attccatgtg ccttccgggg aatgtcggac
    11281 gtccgcgcct ggcggcagcg atcgcttcca cggtcatgtc cgaaccgtat gcgacgcaag
    11341 cggttgccga ccggatcatc gcgtccagcg cgggcccgcg tccaggcgcc accgaaaccg
    11401 gggcatcccg ttccaggagc atgatcgaat tcacaagcag tgccggaaat acggtctcgg
    11461 ccgacgacgg cttcaggatc accctcattc cgccggccag gccatggaac agaactttgg
    11521 cggcgagggc cggtatccgg ccggcaagaa tcgcaagcag atcggccctg tgcggagtgc
    11581 ggccgcgaaa ccttggagcc ctggtcggaa cgaacagccg gtcgatgctt tcgtcgtcaa
    11641 acggttcgaa tgccagcctg atggcccttg caaggtgcat tccggcccat cctgtggagg
    11701 cggcaaggtc ggaaatgtcc cgggcgtcaa gaaacgggcc gggaaccagg ccatccgggc
    11761 ctgccgaggc aagccagcga tccctgatcg tcacaagcag gcttctggtt tcgttcattt
    11821 ccatggcgtc accggtgtct tgcctttacg aatgatacac cgtctgccga ttcctgttca
    11881 cgcgcctccc ggtcgggggg ccgcgttgcg ggagcatccc cttgccgcgg caccggcggc
    11941 cctgcggata tcgcagaatc cgtttccgga tctggtcgcg gtgtcggcgg ccatgattgc
    12001 gacgcatgtg tggcagcagg caaggtcgta gtggacaagc aggccgcgtt cgccgtcggg
    12061 cacgtccttc attgtccgtg gatcaataac ccttgtcctc atccatggaa ccgggatcag
    12121 tcttcccgga acaccgctga ttcgcggcga atcgtaagcc tgtgaaaaca actcggtcat
    12181 tccgaattcc tcgatgacgt cctgcggggc gattcccagc agccttccgg cgtcggcggc
    12241 aagctgacgc ggggtgatgc tgcgacccga cgccttcggc ccgcccgtgt gcatcagcct
    12301 gcttccggca ggaagcggga tcggggtcgc gttcataacc aggaactcga aaagcgacag
    12361 gaagtccagc gatgtgccca gcaggaaaac gcgactaccg tccgaaacgg atcgagaaag
    12421 gatggacagc atgccatccg gatccaggcc ctgggtgccg gcgacgaagc cccccagtcc
    12481 atcgtcgaat ttttcgacca ggaaggtggc catccatgaa agcgaggagt cgggccttgc
    12541 cgaggccggc gggataaggc tcatgaaacg tgtcggcggg tcgtcgtgca tgcagaacag
    12601 ttcgaagccg gccagtgccg atgccttgta tatttccatg tcgagcacgg gcaggcggcc
    12661 acggttttct gaagacgtgc ccgaggttct gaattcagca accgcttcgg acggcggaaa
    12721 ctgggccacg gtcatcatct tgaaggccgt ctccggaagc ggctcgatct tgtccagtcc
    12781 acgaaggttt gcggaaagcg gcgaagttcg tcgcaggcgg ccctgagcgg ctgcaatctc
    12841 gacggccagg gattcgaagc cctcgtcgtc gattccggaa tggccgcgga cggaatcgcc
    12901 aatcatcgtc attaccgcgt caaccaggga tttatcaatc attcggccac ctttgccagg
    12961 gaatcgccga ttatgcgcaa cgccgttccc aaatcatcgt cgccaataac cagaggcggc
    13021 atcagcgcca ggacgtcgga gtcggatccc tcggcaagca gcaggacacc tcttgaaaga
    13081 gcctccgaca caaccttccg tgtcacttcg gaagcgctca tgcccctggt gccggccagc
    13141 gacactccca tcatcgcgcc gcgcccggcg acaccccgga cgaaatcgaa tttccccgcc
    13201 caggatctgg cggcggcggt aacggcgcgt tcgatccttg tggccgcttc cagaggttcc
    13261 atccgcttga tcgcggcgat ggtcgcgatt gccgcggcgc aggaaaccgg ccagcccatg
    13321 aatgtcgacg tgtggacggc ctcgccgtgg tcgggcagga cggcgtccgc gactgccggg
    13381 cgcatcattg cgaccgatat cggaaagccc ccgccaagcg cctttccaag gcacacgata
    13441 tcgggaacga cagcttcagg ttcgctggca aggaaagtgc ccgtccgcca agtcccgcaa
    13501 tagatttcat ccgttatcag aacaatcccg ttccggtcgc agatcgaggc cagtcgcgac
    13561 aggaaagagg gcggtggtac cctgacgcct cctcggccct ggatcggttc cactatcagc
    13621 gcgcccggcc ggccgatgtc cgccccgaac ctgttgatga tcggcgaaga tgccagcctc
    13681 tcgacggtcg aaagaaccag ttccagattg cttccatccg gttccggcca tggcgcgaaa
    13741 acggcccttc cggaaagcat ggcttcgaag ggtttcctga aggcgggtcg ccatgtcgct
    13801 tcaagcgccc ccgcggaaag tccgtggtaa gccccttcaa aagatacgat gccgggcctg
    13861 ccggttgcgg cagccgcgaa tttcagtgcg gtttcgacgg cgtcagcccc gttctgcgaa
    13921 aggacgccgc ggtatccttc ggccggcatc atccccgcaa gcagttgcag gaggccggca
    13981 cggccagggt cgggatacac gtcgcccatc gacgaaacca ggtgtccctg ggccaccctg
    14041 acggcggaca tgacggccgg gttcctgtgg ccggcaaggg ccacgccgaa gaacgacgtc
    14101 atatcgatgt acctgttgcc atcggcgtcc ttcacccttg cgcccctggc cgacccgata
    14161 accatcggga agcggtccgc aacagccgga tgcggatttt caccccttcc cgcctcgagc
    14221 ctgtccagcg cacttttgac ccgggctccg cgaaccgcgc gtctcgattg accgaaggta
    14281 ccggccatgc aagcctccat cccgttccca aaggttactt tttcggaatc acccgctccc
    14341 aggccggggt gaaatattcg tcgccgtcct tgaagacctg tcgaaccccg gaaccgtccc
    14401 ggttcattat gaacagcttc ttcctgccgt cccgtgtcga tgtataggcg atgaaccttc
    14461 cgtccggcga ccacgaaggc tcctcgttgt tgaaattgtc ggtcgaaagc cttttcgaag
    14521 ttcccgaaag gacgtcgaac gcatggatcg agaatcggga accggcgccg cgtgactggt
    14581 aaaggatggt ctttccgtcg ggagaccagt cgggccccgt gttgtagtcg ccgtcaagcg
    14641 gaagcggccg ggcaccggat ccatcggcgt tcatcacgaa gatgcccgga ctgccgccac
    14701 ggtcggaaac gaaggcgatc tgcttcgaat cgggtgacca ggaagggctt gtctcgatgc
    14761 ccggccagga ggtcagcctt gagatgattt cgccgcttac cgggtttacc aggtagatgt
    14821 ccgacgcgcc ttcgtcggcc catgttaccg cagccctgcc gccgtcgggg gcccaggcga
    14881 ttccggtgtt cattccgggc cggttgccga acctgacgcc gtcaacataa agatccgggt
    14941 tgtcgttccg gtagcccgtc catgccagcg aaccgcccgg cgcccatgcc ggcagcatgg
    15001 ccagcttgcc gtcggaactg atccttgtct gttcggccga tccgacgcgg gtgacccaga
    15061 tttccttcac gccgtgcggt ggcgtccttt tcgaataggc gatgcggctg gatgcggccg
    15121 atggaatgcc tgaaacgcac tggccgacgg catcgacgaa gcgtccgcag aaagatatga
    15181 gttcatcttt cctgacggtc gcggtcgaac tggaaacctg cagggagttc ccctcttccg
    15241 tcagccatgc cgacagggag acggtcagtt tttccttatc ggtcgtatcg ccggacggcg
    15301 gagcaatggt ggccgtgacc accatccagt tgccggcgct tcgccagccg aggtaatcaa
    15361 aggcgaccgg ggatgcccag gtgattgcac cgcctgccgg tgcaatatcg cggttgttca
    15421 ccataaagat gcccgtcgcg gcaagttgtt cggccatcgc gtcagccatc ctgaccgcgt
    15481 cgggttccag ttcgccgtaa agcgtcagat cgagcaccag cggaaaagcg accgggatca
    15541 tcaccggttc cgccagggac cagccgaact ttccacagcg ctgatcgatg gcttcgggaa
    15601 ccagcccggg ccgcgggggc tgcgtcgcag gtctgggccg gggagcctgc cccggcagaa
    15661 ggctttcgat ttccacgtcg ggctcggact gggccccggc atcaaaccgt ggccactggc
    15721 cgtaggcgcg accgcagcag gtcaggacga cgaacgtcgt gaccgtcatg gcggcagtct
    15781 gtcgggccga agctggtact ttacgggtca atcgtggcct acttccgaaa cagtgaaccg
    15841 tcgagatcga cgacgatacc acgcttgttc acaagctcca gcgtctttga gtccggcgat
    15901 ggcaggtata cgttgccgcc gtccttcctg gagaaccgtc ttatggcgaa gagagccgcg
    15961 ttgttgaagc tttcttcgcc cgaccttgaa agaacatcga aatccaggat ctggccggcc
    16021 gcgtcaatcc tggtgattct tatcctgacc cgaagaacct tcaactgggc cggcttcagg
    16081 aaggggggga cggtgaactg ggcccttatg gcctccccga ccttggcggc ataaatgcta
    16141 ccttccctgg cttcggttcc ggttccggaa gtcgaaccct ccgcgtgacc gacgatccga
    16201 tcgagggcga ccgccctggc gcggggatca tcatcgtccg gggcatcgcc aaggatgtcc
    16261 ccgaggtcgg tcttcttgcg cctggagtcc gtctgcgcct ttttcggccg tacgtccttc
    16321 ttcaacggct gttcggtttt ctggttgtct ttggagatat ttattgattc ttcaatcttt
    16381 tcagcctgtt catatttgag gaaacgcggt agctggccgg gaaccggttt ggcaagacca
    16441 agtttcggga tgattgacgc ctcgatgatg ccggcgtcca tcgtgtccat cgagtcggcc
    16501 ccgcgcctgt cggacatgaa aggcggctcg gcgcaccgga ttccgtcgca catcgatccg
    16561 gttcccggca tcccgggttt tggcggaaga gcggcattgc cgccagcggt cgccagcatg
    16621 acgacgatcg cgacgtgcac cagcgccgtg accgccagcc cggcctggac aggcctcctg
    16681 tcggaaacgc cgagtgcctg ggctatgtag tcagaaatcc gcatcaggac attccagccg
    16741 tcacatcacg catcagccgc cctcgttgcc accctcggcc tcggaaacca ggccgaattt
    16801 tcccacgccg gacgatcgga tcctggccat caccttaagt gtcaggccgt aaggaaggct
    16861 cctgtcggcc ctgagataca gttccttgtc gttttgaagc tttgcgttcg ttcccagctt
    16921 cgcgacgatc tgatccaggc acggcccgaa cgcagcgtcg tccttgactg accagatcct
    16981 gcggctgtcg cccgtcaccg tggccttgat ggccggcgcg attgaaaggc agtcgagaat
    17041 catggtttcc ccaacgaaaa atttgacgtc cggggttatc gacaggacca gtttctgttc
    17101 ttccgacagg ttgaccggct ccgcatcggt ccgcggcagg ttcaccggaa ccttctggtt
    17161 caggttcgcg gtctcgctgg tcttttcgat tcgctgtttt tcctgctgca cctggatcgt
    17221 ctcgatcgac gacgagatca tgaaaatgat cagaagaacc agcatcacat cgacaagggg
    17281 cgtgacgttg atttccgcca tcatgccccg gttcgagccg cttgaaccgc tcatgcccat
    17341 tggaacctcc ccggcgcggc cggtccgcga atcagcgatc cctgaagaag tgccgtttga
    17401 ggatgttcag gaagtcgatg gagaagttgt ccatctcgac cacgaacacc cggatcttgg
    17461 aattgaagta attgaaggcc atgacggccg gaatggcggc cagcagcccg atggcggtgg
    17521 ccacaagcgc ctgggcgatg tggggcgtga ccgtttcgag gatcggcctg tcgggcgaga
    17581 tgtacgcgaa cgcgttcatg atgccccaga cagtgccgaa caggccgatg aacggggccg
    17641 ccgacccggt tgtagccaga aacggcgtca gcttctcaag cctggtcgtc tgttcctgca
    17701 cggcccgtcg aagggtgcgc tcgatattct cgaagccgta gtcggagccc tgtccgacag
    17761 gcccggtcgt gccctgtcct tcctggttcc gcttcatttc ggtgtatgcc gccgagaaca
    17821 ttctggccac aggcgaatcc gaaagctgcc tcgatgcctc gcgcacctct tcaaggttgc
    17881 tggaatgcca gaacgccttg atgaattcct cggtctgcct gcgaatcttt ctgaaagacc
    17941 agaatttgac gccgatgatg aaccagcaga ccagggacat ggcgatcagc agcagcagaa
    18001 ccatcaggac catgcccctt gatgccagca cctgctgaaa cacgttgatt ccaaaagact
    18061 ctgcggcgcc gggttccatc gcaccctccg aaacgggaaa agccggccgg attatagcat
    18121 ttgtggtcat aagacctgaa accggcgcac acgggttcgt tgcggcgcga tggctggacg
    18181 ttcgttgact tgatgaagcg gtgctgtcat tattcggccg cggccggcaa cgttgccgcg
    18241 tggccgggag cctcaaggta accgcccgtt gttacaggat cttttttctt ggtgattcga
    18301 tggttgaatc cagttcgatc ccgcatccgg tcacggcggc ggttgccgat gcggtcgatt
    18361 cgctgcggct tggcggaatg gtcatcatcg ttgacgacca gtcgcgctgc aacgaaggcg
    18421 acctggtgct ggcggcggac atggtcacgc ccgaggcggt caaccggatg acgaagattg
    18481 ccggcgggct tgtatgcgtc gcgatgactc acgacatggt cgatcgcatc ggaataccga
    18541 tgatggccag caacagcagc gcccatttcg gatcagcctt cacggtttcg gtcgattcaa
    18601 ggctcgggac caccaccggc atctccgcat ttgaccgcgc gagaacgatc gaggatctct
    18661 gcgccgaaga cgcgtcgatg caggatttcg tggtgcccgg acacgtgttt ccgctgcggg
    18721 cgctgccggg tggcgtgttg aaccgggccg gccagaccga ggcggcggtc gatctggtgc
    18781 ggatggctgg ccgcaagccg gccgccgtca tcagccaggt tcttggtgaa gacggttcca
    18841 ttgctggcct taaacaactg ctggaacttg gacgcgcgaa cgggattccg gtcgttgccg
    18901 ttgccgacgt gattgcctgg aggatgcaga aggatctgct tgtgagggcg gtcggttcca
    18961 ccatggtcga taccgagtgg ggccggttca agtcggttgt ttacgaagat accatccgca
    19021 gggcggccca cgttgcgatg gtcatgggcg agccgggccc cgggcccacc ctcgtccggg
    19081 ttcattcgga atgcctcacc ggggatgttt ttcattccct gcgttgcgac tgcgggctgc
    19141 agcttgacgc atcgatgcgc cggattgcat cggaaggcgg cgtgcttctg tatctccgtc
    19201 aggaaggacg cggcatcggc atcgggaaca aggtgaaggc atacgccctg caggatcgcg
    19261 gcctggacac ggtcgatgcg aacaccagcc tcgggttcgc gccggatctg cgggattacg
    19321 gaaccggtgc ccagatcctg gcccaccttg gacttcggaa actgaggctt cttaccaaca
    19381 acccgagaaa gatggtcggg ctttccggat tcgggctcga ggttgtcgag cgcgtccaga
    19441 ttgaactttc gcccaggagc gacagggatc ggcactacct ggaaacgaag aaatccagga
    19501 tgggacacct tctggagaag gtctagcccg gtacggctgg cgacacgttc aggcccgtcg
    19561 gggaaccggc cttcggcgaa tcaggatacc tgtgttgcac agggcactta cttgaagtac
    19621 actaccgccc gccttgcgtt ttccaggagt cattgaatgc tggctgagct caggaaacat
    19681 tccaaatcgg taattatcta cgtgctgttc ggcatcatca tcgtagtgtt cgtgttcacg
    19741 ttcaacatga gcagtcgtcg cagggattcc tccgtcggct atggacgtca gtacgaaaac
    19801 gccgaactcg tcgtcgtggg ggattccagg attgacgtgg tcgatctcga gatggggctg
    19861 cgactgacga cggacgcgcc gctgcccggg aaggagcgca ccgcggaatc ggagcgccag
    19921 gaaagggcgt atcggcagta ccgattccat gatttccccg gcgacccgtc gatggccatc
    19981 ttcggtcagg atccgatggg cgtcgcttca atcaagtcgc ggaaggtcaa ggaagatctg
    20041 gtcgagacct ggctggtgtc cgaggaagcc aggaagaacg gcctggtcgc gactccggac
    20101 gaggtcaggg aacgcatact caagcgtttc cgggatccga caaccggcga attcaagcgc
    20161 agttattacg agaactgggt tcgttaccag ataaggacga cgatgcccag gttcgaggac
    20221 ttcgtggcgc gcgaaatcga gcgcgaaaag atgatctccc tggtcacatc ggcggtcgca
    20281 gttcccgagc gcgaggcgaa gtacgttgcc ggcctgcgca agaacgcccg cagttatgaa
    20341 ttcctggaaa tatcaccagc tcttctgggt gcggcccttg cgggaaacca gaaggaactt
    20401 gccgcgtggc agaaggccaa ccagaaggcg gtgcgtgagt atttcgatgc caataagtcg
    20461 gaattcgctc aggaagccgg acttgatttc cacgttctca agtattccga ccaggcccgc
    20521 gccaccgagg tcttcgaagg cttgaaggat ctgaagggcg aggaacttcg cgatgcgatg
    20581 accgccctgg ccccgacttc ggaggacaag gtcacgaacg agattggcgg ccgttcgcag
    20641 gcagcgctgt cggtcggtgc catcgccagc ctttacgggc aggaagtggc cgatgccgcg
    20701 cagaagctga ccgaattgga actttcgccg gtcgtcaagt ccgcgggcgg gtatttcctg
    20761 ctgatgctcg acgcgcgtcg cccacaggtt gaaccggtgt tcgaggaagc ggccgaggtc
    20821 atcggacgca ggcttttcgg agaacagaag gccgcggccg agatggaagc cctttcgacg
    20881 gccgccatcg ccgccctcaa cgcagccccg gaaaggcccc tggccgaagt ggcggccgaa
    20941 ctgaacgcga aatacgcgcc gcattcgccc gtaagggtcg gagagaccgg cgaggttcgc
    21001 aagcttccgc cttcggtcat gggattgttc atgtgggaac cggacacgta caggggcatc
    21061 ggaaagggca agtccatcac gaccgccctt gaaggcctta ccaacgaatc caggcttctg
    21121 cagcagccgc tgaagccgga gggcggaacc ggcgttttcg tggtcaggct tgccggtgaa
    21181 gtgaaggttc aggagccgac ccgggaggaa atcgacctgg tcgcttcgga actcgccctt
    21241 ttcaagcgtg tcgccgtctg gcgggactgg ttcgatgccc tgcgcgccgg tgccgcatcc
    21301 tcgggcgaac tggttgaaaa ggaacttctt gcaaagatga tcgccgacga ggaaaaggcc
    21361 cttgaacagg ccgtttccgg aaccgcgcct gccgattccg cgaatgcggg cggcgaagaa
    21421 acccagaaca actgatcaat ccccccggag ggcacaatgt cttcattccg tattcgatcg
    21481 aatgatccca tcgcgccttc cgtcttcagg atggaagtcg aggcccccga catcgcgaaa
    21541 taccgcaggg cgggccagtt cgtcatcgtc catccttcgg gcggtcggga aaggattcca
    21601 cttaccatcg cggacgcgga tccggtcgcc ggaaccatca cgctggttta tcaggtggtc
    21661 gggcgcacaa cggcagagat ggccctgctt ccggccggcg gcgatctcgg ggatgtggcc
    21721 gggcctctcg gcaagccgac gcatgtcgag aatttcggga cggtcgtctg cgttggcggc
    21781 ggtatcggcg ttgccccgtc gcatcccatt gcccaggcca tgaagagcgc cgggaaccga
    21841 gtaatttcga tcctcggcgc ccggaccgcc gacctgatca tcatggaaag ggagatgcgc
    21901 ctggcttcgg atgacgtaat ctgcgtcacc gacgacggca gttatggaag aaaggggctt
    21961 gtcacggatg ccctgaagga tttgctggat gatccttcgg cgccgattaa ccttgtggtc
    22021 gccatcgggc cgcctgtcat gatgaaggcg gtttccgaac ttaccaggcc gtacggcgtt
    22081 aagactttcg taagtcttaa tacggtcatg attgacggaa ccggcatgtg cggcggctgc
    22141 cgcgtaaccg ttggtggaaa gacgaaattc gtatgtgttg acggtcccga atttgacggt
    22201 cacggcgtgg atttcgatgg gatgatgcgc cgccagcgga tgtattcggt cgatgagaag
    22261 gcgtcgtacc aggcttttct gaaggatcac ggttgcaggc ttgaagaaag aatccgggag
    22321 ctgaaacgtg gctgaaaaac ttacaccaag gcagaggctc gcgattccac cagttccgat
    22381 gagggaacag gatcccgggg aaaggcgccg caacatacgt gaagtacctt tcggttattc
    22441 cgccgaagag gcgatgcttg aagccagccg atgtctgctg tgcaggaaca agccctgtat
    22501 cgatggttgc cccgtcgccg tcgatattcc cggtttcatt gaactggtgt ctctcggcct
    22561 gttcaatcag gccgccgaat tgatccggaa gacgaattgt cttcccgccg tctgcggaag
    22621 ggtttgtccc caggaagagc agtgccaggc ggtatgcacc gtcgcgaagt cacacaagga
    22681 tccggcgatg agtgtccaga tcggcaagct tgaacgtttt gttgccgact gggatcgcga
    22741 gcatccggtg gaaggggctg tcgcctcgga cgtcacgccg accggtcgca aggtggcaat
    22801 catcggttcc ggacctggcg gacttacggc ggccggggat cttgccgatg ccggccacga
    22861 agtgacggtg ttcgaggctc tgcacaggac gggcggcgtc cttgtttacg ggattcccga
    22921 gttcaggctt ccgaaggaca tagtcgagtt cgaggtcgat ggcctggtca ggcgcggcgt
    22981 caggattgtc acgaacgttg ccatcggcaa gtcgaagtcg atcgacgatc tgttcgccga
    23041 gggttatgac gccgtgttca ttgcgaccgg tgccggtctt ccgtggttca agaacattcc
    23101 cggcgaggat tcccttggcg tgtattcggc caacgagtac ctgacaaggg ccaacctgat
    23161 ggatgcgtat cggttcgggg acggttccga cacgccgatc atgcgtgggc ggacagtcgt
    23221 cacctttggc ggcggaaatg tcgccatgga cgccgcaagg acggccttgc gccttggcgc
    23281 ggacgagtca atcatcgtgt atcggcgttc ccttgccgag atgccggcca ggcttgaaga
    23341 gatccatcac gcccaggaag agggcgtcag gttcgaactg cttcaggatg cggttgaggt
    23401 tgtcgcggac gatgccggat gggtgaccgg tgtgcggtgt cagaagatgg cgctgggcga
    23461 acccgacaga agcggccgcc cccgcccggt tccaatcgag gggagcgagt tcgtcatgcc
    23521 gtgttcggtc gtgatatgcg cgatcggaaa tggagccaac ccaatcctta ccgaagcgac
    23581 cccggcaatc gaaacgggca ggtggggcaa catcgttgcc gatccggtga ctggaaggac
    23641 ttccatgaag ggggtctggg ccggtggtga catcgttcgc ggcggcgcga cggtcattct
    23701 ggcgatgggt gacgggcggc gtgccgcagc gtcgatcaac gaatacctcc agaccggcgt
    23761 ctggtaggtc ggtgtccttc acggcctgtt tcggtgccgt ttgagccggg gtgccacgtg
    23821 cttgaactgc tttgcggaat cgtgtgtctg ttcgccggtg tcgccgggat gatctgggtc
    23881 atccgggacg gcaggcgtgc cgccgatttg atgtcgcgtg ccgcggttgt cctgcaggtc
    23941 gcggtcgtcc ttctgctgct tgttgattgg ggccattcct gggtcgagat gtccggaatt
    24001 gacatcgtga ccggccggtt cgcgtggctt gcgctgccgc acgtccttgc ccttgtttca
    24061 cttgtcggca tgctggtata tggccctgat ttccgtggcc gtgccggcgt tcttgcaacc
    24121 gtccagaccg gatcgctgat tggtgtgatg ctgctcgccg agcaggagat cgacctgagt
    24181 ttcctggccc ctgtaccgct ggtctccctt tatctttcgg caatcgtcgc gttcgctttc
    24241 ttcggttcca gccttgttgt gctgccccgg cccgattcct tctggtaccg cttcgtgttc
    24301 aaaccccgcg aaaacctgct ggcttccatc gaggcgctga aggaatgcgg ttttgatgtc
    24361 accaggccgg aaaccgtttt tgagtgtgga tcggcgagcg ggtggcttga aggcaccatg
    24421 gtgcacatct ccaccgcgcc gtcgctttat ccgctccgat acgggctcag gatcaggatt
    24481 gctgtttcaa ggacgtggca cggccgccgc cttccaggtt ttgccgagaa cgagaagtgg
    24541 cttccggggc tgggatggat tgaatacagc gg
//
LOCUS       DAOPPA010000003        22792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_2819_length_22792_cov_3.575846, whole genome shotgun sequence.
ACCESSION   DAOPPA010000003 DAOPPA010000000
VERSION     DAOPPA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 22792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22792
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_22792_cov_3.575846"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..919)
                     /locus_tag="PKH54_00220"
     CDS             complement(89..919)
                     /locus_tag="PKH54_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98338.1"
                     /translation="MPETMPMPTDLTSKCIIRFAGTMLLSGLLICPAAGAPATTDDAS
                     SSVVLIPHPHIGLGDVRSEAVASATRLLGLKESFTRDSFARHILFVNGIMEYSSLPAK
                     SWARDLAKKLKKNGILSRKGTPAPGDLVIFGLEPSAPGSINTRNVMVGVVEKVHSDSL
                     TFITAGSKKIIRGSMSLKMGRGKDTKLASCQVTERKKVASKKPPPGSGKKKSGRNTAS
                     KSGGSKITTVTKQVPCRASELFAGFADIEAVVRKLGKRPTADRTVTIFDDERPESLED
                     "
     gene            1030..1746
                     /locus_tag="PKH54_00225"
     CDS             1030..1746
                     /locus_tag="PKH54_00225"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC98339.1"
                     /translation="MERDRTLFPIPGILILAGFLSGCAHVAPEGAPGFAGADPGGPSV
                     QAAPDSGHGPSRRFFSIDGPIVERRAFEIPSGFVRMASSGETSSRRQAVVFAYQPRRI
                     SCGDAWKIAKTVGVEAGIDPGVIFGIMRVESRLAVNAMSPAGAVGLMQVMPGPADVLG
                     CGDRLDPIENSRCGVIILSRFLKRYDNNLVLGLSAYNAGFGMPSRARRKNSIPANFVY
                     AEKVLRVRSRFLLYGCTPWD"
     gene            1851..2747
                     /locus_tag="PKH54_00230"
     CDS             1851..2747
                     /locus_tag="PKH54_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98340.1"
                     /translation="MARKKSRNTPEEKSRDLNPATPDVPAVDVAPGDASGPEVPDVPA
                     VPSVPDAPPSMMAPPPPPSPTSSMKAFDLPPLHVGEGHAQGKGNVLKGILERTQQEAE
                     KEAARLMDSLKVQQDAQRMAREEEDRRKAAEARAKVEEERRRREAALKEYEDRQRRKE
                     EAERARTAPAPRETVSMQASKGGRGWIVGLAVAVVVLGGGGFAGWKIMNMVSPTAFNV
                     DSPIERARGGAVVTTPIVYGPSTLKLARSVDAELLVAGIAPAKYEAEAPAPVVRKTGK
                     APQEKKIINIRTGILGGKKVIR"
     gene            2781..3701
                     /locus_tag="PKH54_00235"
     CDS             2781..3701
                     /locus_tag="PKH54_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC98341.1"
                     /translation="MNAQTSHGSRPVAAVLLMVASAASFALMGAAVKASTGSLPFLVS
                     LFFRSILGIIPLGIIVIVRQGSLRSNRQKLLFRRSLLGFCAMFMFFFAIEHLPLSTAV
                     VLNQSAPVFTVIFSAILLKEHRAIHVLPLSFVAFLGVGVLVGPDIAETGIEASIGLAS
                     AVLSALAYVTVKQLSVTESSPLIVFYFSVWGTVFAAVVLGLALLTGWGDLDLDVVARE
                     LSRPAGVVALCLVGFFGVCGQLLMTASYARARASLVSPFAFFNPLFSYVIGVSVFSES
                     MTLHGVLGGILVVSASALVPFVSNGATRAR"
     gene            3765..5678
                     /locus_tag="PKH54_00240"
     CDS             3765..5678
                     /locus_tag="PKH54_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOC98342.1"
                     /translation="MQKFKWFPWAALLAVACAGDRAPVDEGAWVASWCPPESAEFSPV
                     HVTPLPLQSMEWGELMESGAFPGGASVAFLPADDRERAMMHEFIAFSGIPEGTSEEAP
                     IFLQVLEFEAWDEAATECGHPKPSSGEAYILSVQPTTDGNVAIRLVAPNESGLRMAFL
                     TAAQVVRAMVESPEATRRRMLFLDAPAIDGRGVIETFYGAPYLEEERRALLPWLGRLK
                     FNQYIYAAKMDVWVNWIGPYWSEDFNSFDAGYVAFLERLCADIKASGMVPGIQVRPLD
                     QLVFSSSEDVARFVDKVAFFQSLGFEIFSLSFDDTDKILCSQDQAAFESYDDAVTAFS
                     ASAFDAIRARMPDLKLGWVPNDYYTNDPDAASQLPLAGQRLPQYVSIGWTGNEVIPAA
                     ITADDAARVAAWLRRKPLLGDNYPVWEPTATRYFLGPLVGRSADLAGALDSIMFNPNP
                     APFPSLPALVTCADYAWNPTGYQPEAAIDRMAAFLAGKSPAATALVILARHNRSHLFH
                     GSMAPELAPLLSQFWVDFSDGNATLNSSAVKTIFSDLAGLPSSWSVAHLPEPLFLSLE
                     SFVNQAGRMGVAGMDAVNLLSSIAAGLEPEPSIIESFNQTMAEIADHPEEPSGDVIVG
                     FLEQASMAINVSN"
     gene            complement(5688..6758)
                     /locus_tag="PKH54_00245"
     CDS             complement(5688..6758)
                     /locus_tag="PKH54_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98343.1"
                     /translation="MMNVKSVMCRYSLIPLLAGCLFIVMFAGCGPTFVRDIGREAMPR
                     NIPVSKAHDCRVFVAPVADERPEYEKVGENKMGTNMALPLLVYWYFSYDGPIYQDATN
                     LGGEAVPESIRTGLKTALNTSGVCAVVQSQGDADFVITPILKHAYGADYKKSNFWVLA
                     LSGGAVAVTNDYHFYPLGSVVMDFKVTQAKTGSPVTEFGISEGTIFDKNQPGSSIAFS
                     ARGHSRFQMDFNRANELVMSFHKVFTKFPRQVELALMATGKIRSGAFDDKKFAIARMD
                     RGYEFMEWALVDAATGRVDSATQVRRYLPVFAPPGKWVVLPWQDGLLNHEEYMNLLTR
                     LSSRFDIKWEDNLTAATFGGLK"
     gene            complement(6761..7447)
                     /locus_tag="PKH54_00250"
     CDS             complement(6761..7447)
                     /locus_tag="PKH54_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98344.1"
                     /translation="MMKRNTLERLLLTAMLLFATMLLACTPKYGMLHLQPMKIWENAV
                     AAAGDEDKVSFREASRLQAPDSPPTVVVRLFADTILAPTGVYLSDEESHITETSEMYF
                     NEHMSAVVFDYFVEQYQAAGIPTFRMYSSEAPSKCAGGEAQTLRILELTIHHAELHRW
                     KQKADEGFKLVDLVAFTYSYAWKDCTGKALYSSEVISQDFVSAESADELFTVASIMAV
                     ETAGKLAGTR"
     gene            complement(7444..7848)
                     /locus_tag="PKH54_00255"
     CDS             complement(7444..7848)
                     /locus_tag="PKH54_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98345.1"
                     /translation="MHKTTVRRLCVSICLISSVSLFLLALAGCGPKAIYPANPPQGAK
                     WGTFHQRFLKVRTVAVSSSSSSGGTTTTTTQTSQNANQQYVDAPFNSPLEPAVLSAGE
                     TVTEDVLKGINPTSSNISSGSTLAGLAAPGVK"
     gene            complement(7990..9162)
                     /locus_tag="PKH54_00260"
     CDS             complement(7990..9162)
                     /locus_tag="PKH54_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOC98346.1"
                     /translation="MNGNNGPRYSRRKALLVLTASVASVIVAALLVLDMIPGLRGDPG
                     ASDSSNVASRSGFLYAGIPARIEFMTDTGEAIRMNLKNVPDGDLRRGGLSTAKRLDGA
                     WDILVRTGSVFNPFDPSSELAILNSNQTTSPVQVSDWMAQVAGSATTVSQMTSGAFDA
                     TVRPLKTIWKNAAQSGVLPDEADITHALGRMGWGYVTLLRDGEGKWEIARNRPAMELD
                     FGGIVKGWSVGMAMTFLQDNGVKSALVQVGGEIALAGNSPSGRPWRIGIRHPLEPGSN
                     WTILNISGRTAVSTSGNYEQPVMIGNTAYYHIFDPITGRPIPTDVLGTTVVVSGGPNP
                     NAIADGLATAFAVMGPDRAIEAAAKMEGVEVLFIVRDPASGAPKERTTPGFDRFRH"
     gene            complement(9149..10330)
                     /gene="tgt"
                     /locus_tag="PKH54_00265"
     CDS             complement(9149..10330)
                     /gene="tgt"
                     /locus_tag="PKH54_00265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOC98347.1"
                     /translation="MIDCGFRFELLMTDSRTGARRGRLHTAHGTIETPCFMPVGTLGT
                     VKGMLPRDVAEAGFGIILSNTYHLALRPGPDIVAMHGGLHRFMNWDRAILTDSGGFQV
                     FSLSDRRRINEDGVSFKSHINGDTVEFTPESVIEIQQKLGSDIAMVLDVCPPSTAGMD
                     ELDRAMELTTKWARRCQDARTSRNQAVFAIVQGGLDIERRRQHAAALTPLGFDGYAIG
                     GLSVGEPPHQMYPVAASTAGVLPFDKPRYLMGVGMPADLPNCVMGGIDMFDCVFPTRC
                     GRNGTYLTSVGRLNIKNTRYRDDLGPIDPECDCPVCRNFSASYLRHLFVAGEILSNVL
                     NTMHNLHYYWKLMEQVRNAITEGTLPELAARLNTTHGRDPMVGSVCESATSGGDPDER
                     E"
     gene            complement(10371..11453)
                     /gene="queA"
                     /locus_tag="PKH54_00270"
     CDS             complement(10371..11453)
                     /gene="queA"
                     /locus_tag="PKH54_00270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOC98348.1"
                     /translation="MSDTIPPEFQLEMYDFDLPQDRIAQVPAQRRDGSRLMHLPISGG
                     APTDHVFSDIVGFLRPGDLLVLNDTRVFPARLFARKPAGGLVELLALATAEYGFSAMY
                     GTHRGLKAGTRLSVLDRDSNDSGLRVIVTSTDRGVAHIRMECDGAQATCSAAELFDRF
                     GHMPLPPYIKRPDGSMEDLDRIRYQTVYASRTGAVAAPTAGLHFTPAILQTIREAGVC
                     TASVTLHVGPGTFKPVRSADVRQHDVGTESYEISADAARAINAAVDEGRRIIAVGTTV
                     VRTLEADGTSGHVTAGQKDTSILITPGYRFKIITGMITNFHIPKSSLILLVSALAGRE
                     RILDAYRTAVERGYRFYSYGDSMLIL"
     gene            complement(11484..13322)
                     /locus_tag="PKH54_00275"
     CDS             complement(11484..13322)
                     /locus_tag="PKH54_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC98349.1"
                     /translation="MSSEMPIHSASFKRRRFLNWFPMGLTYAFLYMGRYNLTVAKNAL
                     GDLMTKEDFGVIFGAGTLVYAFAFLLNGPLTDKIGGKKAILAASMGSGLMNLIMGLFV
                     WNMIVNPGSVSINLVTAMSVMYAINMYFQSFAAVAIVKVNSAWFHVRERGGFSGIFGT
                     MISSGIFFAFTVNSWILDLTAGLGPAGSKATWWVFLAPSAILFVMFFVELILLRDSPS
                     GAGHDDFDTGDASSGMGDDVKLSFIVKKVLTNPIILTVAFIELCTGVIRNGVMHWFPI
                     YAKEVWALPSSHILRNGSWSGDLWWIIALMFAAAAALFVLSTRFTGKRKASMIISGAL
                     IALAPFIQGGWGGILFVAGVIGGNVAGWVSDLFFQSRRAPAAGGLYVMLAICLLAMYF
                     TLGSTTTTVGWTASPDYPLMKGDKVVSVNGQSGLKDWSQISRAVACIPSKCEGEGVRW
                     DTSACMCTASPRETSDGIATSSGTFQLDVIRDGAPVSVTLKDPLVTARAGDARRLDAG
                     PVLTLTPLFMGLILFLVSLGVIGTHGLLSGTATMDFGGRKGAATAVGMIDGFVYLGTA
                     LQSVSLGFLTTRSWSYWPLFMLPFAFLGVFLLTRIWKARPGTGGGH"
     gene            complement(13337..13918)
                     /locus_tag="PKH54_00280"
     CDS             complement(13337..13918)
                     /locus_tag="PKH54_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98350.1"
                     /translation="MNKTMKLIIGAAAMVVAAAVLTGAFFILERAPAAGSSENHLRDT
                     LGGHQDGPAPIQIRQGALVNPRKRPPRIMESIDRDRARRSLAVPIPTTRDEFNENRRQ
                     EKAAAEAEPRQRKDADPMTVRHGPRPVEATIESAARLQAKGRTDDAERMLRNAILTEP
                     NPLNRGRLEKELEQIRRGSGRKQPTGSQPFKKQ"
     gene            complement(13915..15606)
                     /locus_tag="PKH54_00285"
     CDS             complement(13915..15606)
                     /locus_tag="PKH54_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC98351.1"
                     /translation="MKMLVPFALVLMISTTSIAEPAEPAYPGDDSRVFWFLVMSDQHI
                     TARDSDGPERLEWLLTDCMDIVEPDIIFNMGDLTDASKGEPLVSVHPGQQDEEWQLYR
                     QILDDTGITVDRYIDLPGNHDQYSERGLDHYLQYSLAGTTFGRTQHSVVIDKPWGSYH
                     FMGIDTAGNDGAGFPFDNAGLDLGELMEIQGALQDNTQARLTLMFGHHPIVRPDLGFK
                     LGEGSDEIQDLMGDFNVRAYFYGHTHEYRDLYWPENEWMPTTLHENVDSFGKADENQL
                     LLVSVDNDNLHIRQVSIQEWPWVIITTPSDSRQGESNPWAFDVPAGLESAPVRALGFT
                     ADPFLSCDFRVDDLGWHAMAPLGNHLFQASMDTRNMAQGSHSLEVRCMPGPSTGHRIR
                     FNVVAYTCGNGIDEDEDGLTDWPDDPGCDSVLDSDEYNEPIVIVDDVEPTPDVLEDIV
                     EDAGQDATSPDDVTADVAQPGEDSSPPVDVLEEIGSNDVSVSDAGRDVVADIGPGADL
                     AIDDVDEGNEGDDTTAVKGGGCASASGTADPGLLPAMCLLTLAILAIFRSRRRAS"
     gene            complement(15779..17527)
                     /locus_tag="PKH54_00290"
     CDS             complement(15779..17527)
                     /locus_tag="PKH54_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC98352.1"
                     /translation="MMTRLSLPLLLFAGTLMGTACGGSFDSTWDGPPWPASCRSDIDC
                     EPMQGCFKAAGICVTRYPAGSALTFSVQPNGRLDTPGDQFNGIYMSAPGTLDLQLVPA
                     AIAQGRLGRQEEDEGIEIAPDPGRLVATAEGLIPGLQFRCESGHLDEKNAFAWELALI
                     PDLDYLVTFVPDKRTVPPWHFRLNIGLSGSVQFDLPPKSSISKVRGIVVGSEDGGKFP
                     VGNSIVTALIDGKLVSSSVTDDLGVFDVSVPNLPGQIRIVVAPGESGKVFADREFKWD
                     SIDDFRQALPSTDFLVLDVGSLPVVASISLYVVGLRETAVRVPVAGTRLFIHGDGPDG
                     TYRMHTTVDETSVKLLKLPPGDYRIDAVTPGYGLSSRLRAAQYSMGQRAFSFQDGQSG
                     NFAVELPPRPRISGRIVYSGTDDPIRDARIVFASTAAELLPLETPTDADVVHEAMSDS
                     RGEFLVRLDPGNYAVRVEPFSGSGLAPASWRLVTITGTENIQISVTSGRMLEGTVRSA
                     DGKPLEKALITFFFPTTFEQYGQWPMKTSSISRTIREAGRAITDSNGRFQVILPDPDN
                     PAAAIGAIFTSWSDGQ"
     gene            complement(17524..18012)
                     /locus_tag="PKH54_00295"
     CDS             complement(17524..18012)
                     /locus_tag="PKH54_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98353.1"
                     /translation="MNRASEEIGRTSILGLILPLATLACAIPPEINVRQDGLNPPSLD
                     RSMISPSPDQVVELTSGTIEFSVASAVTNVDAGDQLFFQWFVGYPESATPRPPAFESF
                     DTIQFNPCGFADVLAPLGSTHSMELFITEQPMDFDPVTGRVVPDSYIYVSWPIRLKVS
                     CP"
     gene            complement(18034..19758)
                     /locus_tag="PKH54_00300"
     CDS             complement(18034..19758)
                     /locus_tag="PKH54_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC98354.1"
                     /translation="MIGRDEGCDISLPIPGLKQIHAEVVLDGQGHRIMPMDPKAEIQV
                     NGQKTRMTVLQEWDVIRIGTATMIYVPDDFSFKDTSGREGESFKALEAMSAMGRRLME
                     TTDLDRCLELLLDETLSISRATKGFIIFMESGSPVVKVARNVGMHELPDDAVLFSESI
                     VRKALENGESQLVADAGSNKEFSAATSVINLKLTSVMCIPLKSAGEILGVLYLGTERL
                     ADLFDDVTLKILNVYAALAASIVNSVMLINNLRRDRDALREEVKVSRFGSLIGSCPGM
                     QLVFERVTRIAPTEVPVLVTGETGTGKELIARELHARSNRSRGPFIAINCGAIPENLI
                     ESELFGHVKGAFTGAVTTTAGKFQAASGGTLFLDEIGDLPFHMQVKLLRVLQDGMVMK
                     VGALKPDQVDIRLVAATNRDLEGAIQASGFREDLYYRIAGVVIKLPPLRDRGADIDTL
                     AQYFLKRFSAEFKTQVTRFSPEALAAIRAWNWPGNIREMENRIRKAVLFSDGQVADAE
                     SLEFEKGSQTAELLPLAAARENFERDYILAALQRHGDNKAATARALGVDARTIFRYLE
                     KTQDDKRG"
     gene            complement(19838..20638)
                     /locus_tag="PKH54_00305"
     CDS             complement(19838..20638)
                     /locus_tag="PKH54_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98355.1"
                     /translation="MLLLLVSAGFSMSAAAATVEEEFAEAENTFKFQDYPAAAKMLET
                     LLYPKIRVQDINQQLKAREYLAACHFWLGNKARMEEEFTALLTQAPLHELDPFFYPGE
                     LISQFQTLKLRLIELHILDPEKARAEAKPECTVTVEEVQKRSKLPMLVPFGVGQFVNG
                     KNTKGVLFLTGELVFLGLNIGAWAGAESLRGDDGFYSAADAVTAKKLRIVQYVSLGAF
                     VGLAIWGIADAFKDFVPEKRNLKVIPCPTGQSRSGAPDGIGICFHWRR"
     gene            20744..>22792
                     /locus_tag="PKH54_00310"
     CDS             20744..>22792
                     /locus_tag="PKH54_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98356.1"
                     /translation="MSSWIRYCISVMAVAAFVLSARAQPSPKVPDPAAAPAVDAVSPA
                     AEAGVAPGAPVTEAVSEPAAGNDTPAGRAAEPAAEPAVAASAPVTAAEAAPEPSPPPP
                     PPPPLIPGESVDELASSIQGLCEKISSFEDVMKLDLYARTAIFDRLRMGLYRETLNLV
                     FAYLPCFSGAGRPTAGMEDYESSLRDLACSMPGMIFLNEGSVDAQLARRSLDACPPPP
                     PSPGRGADAEAEWAGAVRRYLSLAAAVLPPPELLRVGAAWVESMGRIWESPLPLKNAP
                     TAVDWRPVDLKHAIRSTEALLVKSTQPGGAPASLPSATDDLGLYVAPGPEVPSDVAEA
                     RLVLVVAALATGDSDAAVRAGGGADIGCRYGMNALLGILEPERGSSPGFLSMVLTPIL
                     ASAPVDNLVKAVKTASNKLGSRCGMVSLLAELAIGTMTFESLPTDPAAIARLASVVAA
                     APPGRPLTGDPAVRVELLQQVVASLDAGTDARGFRLYELGRALLDSGRPDDADDAFTR
                     ARRDLPEVARPLAYQGKLLAALVRSSAVPGDVVLAARNLHDAGPSGESLEALVADIPV
                     GENRNAAIEALWKGAVASGGDAPAMMVDLFIHLLDTFPGSAEAALAISLMEREGVDDS
                     EPEAFAGWLLLKGRHQVVAGDLKGGLATLDQALKLLKGDVPVAREKALPRLVFWLVES
                     G"
BASE COUNT         4337 a         6725 c         6917 g         4813 t
ORIGIN      
        1 tacatgcaca agaaccagga tgttccgcgg gtttgtccag gccagccgac cttcggtctt
       61 cttctcggaa ctgcccactt ccttcgtttc agtcctcaag tgactcgggc cgttcgtcgt
      121 cgaatatcgt tacggttctg tccgccgtcg gtcttttccc aagcttcctc acgacggcct
      181 cgatgtcggc gaatcccgcg aaaagttcgg aagccctgca gggaacctgc tttgtgacgg
      241 tcgtgatttt tgaaccgccc gactttgacg cggtgttcct gccggatttc ttctttccgg
      301 atccgggcgg gggctttttt gatgcaacct tctttctttc cgtgacctga caggaagcga
      361 gcttcgtatc ctttccacgg cccattttca ggctcattga tcccctgatt atcttcttcg
      421 agccggccgt gatgaacgtc aggctgtcgg aatgaacctt ttccacgacc ccgaccatca
      481 cgttgcgcgt gtttattgag cctggcgccg aaggctccag gccgaaaatg accaggtcgc
      541 caggagccgg ggttcccttt ctggaaagaa tcccattctt cttcagcttc ttcgcaaggt
      601 cgcgggccca cgacttcgcg ggcaggctgc tgtattccat gattccgttg acgaaaagga
      661 tgtggcgggc aaacgagtct cgagtaaagg actccttcag gccaagcagc cgcgttgccg
      721 atgcgacggc ttccgacctg acgtcgccaa gtccgatgtg tgggtgggga atcagtacca
      781 ccgaggaact ggcgtcgtcc gtggtggccg gcgcgcccgc cgccgggcag atcaggagtc
      841 ccgacagcag cattgtgccg gcgaatctga ttatgcattt ggatgtgagg tctgtcggca
      901 tcggcattgt ttctggcatg ctccgtcgct tgtatgataa aaccaaatcc ggccgcgtgc
      961 ccagcgcccg tccgtactgg gcgccgaact gtcgcgggtc ggacgtgcgg acgtatggtg
     1021 aaccgcagca tggaacgtga cagaacctta ttccccatac ccggcatcct gatcctcgcg
     1081 ggattccttt ccggatgtgc ccatgtggcg ccggaaggcg cgccaggttt cgccggtgcc
     1141 gatcccgggg ggccatccgt tcaggccgcg ccggattccg gccatgggcc ctcacgcagg
     1201 ttcttttcaa tcgacgggcc gatcgtcgag cgtcgggctt tcgagattcc atccggtttc
     1261 gtaaggatgg cttcgtccgg tgagacctcg tcgcgccgcc aggcggtcgt atttgcatac
     1321 cagccgcggc ggatttcgtg cggcgacgcg tggaaaatcg cgaagacggt gggcgtcgag
     1381 gccggaatcg acccgggcgt cattttcggg ataatgcggg tcgaaagcag gctggcggta
     1441 aacgcgatgt ccccggccgg cgcggtcggg ctcatgcagg tgatgccggg tccggcggac
     1501 gtccttggtt gcggggatcg ccttgacccg attgagaatt ccaggtgcgg tgtcatcatc
     1561 ctgtccagat tcctgaagag atacgacaac aatctcgtgc ttggtctttc ggcttacaac
     1621 gccgggtttg gaatgcccag ccgggcgcgc aggaagaact cgattccggc caattttgtt
     1681 tatgccgaaa aggttctgcg ggtcagatcc cggttccttc tttacgggtg cactccatgg
     1741 gactgaaatg ttcccgattt ttgggttcga cggttgacgc aaggcttcgc agacaataac
     1801 attcgatgga atttttccga tgacctgaaa agtctgtcgg gttggatgat atggcaagaa
     1861 agaaatcaag gaacacacct gaagaaaaat ccagggatct taaccccgcc acgcccgatg
     1921 tgccggctgt cgatgtcgcc ccgggtgatg cgtccgggcc ggaagttccc gacgtcccag
     1981 cggttccctc ggtgcccgac gcgccgccgt ccatgatggc gcccccgccg cctccttcgc
     2041 cgacaagctc catgaaggcg tttgatctgc cgccgcttca tgtgggcgaa ggtcacgcac
     2101 agggaaaggg caacgtactg aaggggattc tcgagcggac ccagcaggaa gccgagaagg
     2161 aagcagcccg gctgatggat tccctgaagg ttcagcagga tgcccagcgg atggcccgcg
     2221 aggaagagga tcgccgcaag gctgccgagg ccagggccaa ggtcgaggaa gaacggcgtc
     2281 gtcgcgaggc ggcccttaag gaatacgagg atcgccagcg gcgaaaggaa gaggccgagc
     2341 gtgcccggac tgccccggcg ccgcgtgaaa ccgtttccat gcaggccagc aagggcggca
     2401 ggggatggat tgtcgggctg gctgtcgccg tggttgtgct tggcggtggc ggttttgccg
     2461 gctggaagat catgaatatg gtctcgccga ccgccttcaa cgtcgattcc ccgatagaaa
     2521 gggcccgcgg cggggctgtc gttacgactc ccattgttta cggcccttcg accctgaagc
     2581 tggcgcgatc cgtcgatgcc gaactcctgg tggccggcat tgctccggcg aagtacgagg
     2641 cggaagcccc ggcccctgtc gtcaggaaga ccggcaaggc gccgcaggaa aagaagatta
     2701 tcaatatccg taccggcatc cttggcggaa agaaggtcat ccgctagctc gctttccctt
     2761 tcattcaaaa gcagatgagg atgaacgcgc agacatcgca cgggtccagg cccgtcgcag
     2821 ccgtcctgct tatggtggct tccgcggcgt cctttgccct gatgggcgcg gccgtcaagg
     2881 catcgaccgg aagcctgcct ttccttgtgt cgcttttctt tcgaagcatt cttggcatca
     2941 ttcctctggg aatcattgta atcgtcaggc agggatcgct tcgatcgaat cgtcagaagc
     3001 ttctgttccg ccggtcgctt ctcggcttct gcgcgatgtt catgttcttc ttcgccattg
     3061 aacacctgcc gctttcaacc gccgtggttc tgaatcagtc cgccccggta ttcaccgtga
     3121 tcttttcggc catcctcctg aaggagcaca gggcgattca tgtcctgccc ctttcattcg
     3181 tggcttttct gggcgtcggg gttcttgtcg gcccggatat cgccgaaacc ggaatcgagg
     3241 catccatcgg gctggcttcc gcggtgctat cggcgctcgc ctacgttacg gtgaagcagc
     3301 tctcggtgac cgagtcatcg ccactgatag tcttctattt ttcagtatgg ggaaccgttt
     3361 tcgcggcggt cgtgcttggc ctggccctgc ttacgggatg gggcgatctt gaccttgatg
     3421 tggtcgcgcg cgagctgtcc agaccggcgg gcgtcgttgc gttgtgtctt gtcgggttct
     3481 tcggcgtctg cggacaactc ctgatgacgg catcgtacgc cagggccagg gcctctcttg
     3541 taagcccgtt tgcctttttc aatccgctgt tctcctatgt gatcggtgtt tccgtgtttt
     3601 ccgagtcgat gactcttcat ggcgtgcttg gcggaattct tgtagtctcg gcctccgcgc
     3661 tcgtgccttt cgtttcgaat ggcgcgacca gggcacgctg attccgtttg cccgtgatgt
     3721 cattccctgt aaaatggccg tgtccgtcgc catgcggtta gaaaatgcag aaattcaaat
     3781 ggttcccctg ggcggcgctg cttgctgtgg cgtgcgccgg agaccgggcc cccgtcgatg
     3841 agggggcatg ggtcgcgtcc tggtgtcccc cggagtcggc tgaattcagt ccggttcatg
     3901 tgaccccgct tccattgcag tcgatggaat ggggcgaatt gatggaatcg ggggcgttcc
     3961 cggggggcgc atcggtcgcg ttcctgcctg ccgacgatcg ggaacgggcg atgatgcatg
     4021 aattcatcgc gttttccggg ataccggaag gaacgtcgga agaagccccc atctttcttc
     4081 aggtgcttga attcgaggct tgggacgagg ccgcgactga atgcggccat ccaaagccat
     4141 cttcgggtga ggcctatatc ctgtcggttc agccgacaac cgacggcaat gtggcgattc
     4201 gtcttgtcgc cccgaacgag tccgggcttc ggatggcctt cctgaccgcg gcccaggtcg
     4261 tgcgtgccat ggtcgagtca ccggaagcca cgcgcaggcg gatgctgttc cttgatgcgc
     4321 cggcaatcga cggtcgcggg gtcatcgaga cgttttacgg tgccccgtac cttgaagagg
     4381 agcgcagggc actcctgccg tggctcggtc gtttgaaatt caaccagtac atctatgctg
     4441 ccaagatgga tgtgtgggtc aattggattg gtccgtactg gtcggaagac ttcaattcct
     4501 tcgatgccgg gtatgtggcc tttctcgaac gtctgtgcgc cgacatcaag gcaagcggga
     4561 tggttccggg aatccaggtg cgtccgctgg atcaactggt attttccagc agtgaggatg
     4621 tggccaggtt cgttgacaag gttgcttttt ttcagtcgct tggatttgaa attttcagcc
     4681 tgagctttga cgataccgac aagattcttt gctcgcagga tcaggccgcc tttgaatcct
     4741 atgacgatgc cgtgaccgcg ttttcggcat cggcttttga cgccatccgc gcccgcatgc
     4801 cggatctgaa gctcgggtgg gtgccgaacg actactacac gaacgatccg gacgcggcgt
     4861 cgcagcttcc gcttgcaggc caacgtttac cgcaatatgt cagcatcgga tggaccggca
     4921 acgaggttat tccggccgcg ataacggccg acgatgccgc ccgcgtcgcg gcctggctcc
     4981 gtcgcaagcc gttgcttggc gacaactatc ctgtatggga accgacggcg accaggtatt
     5041 tccttggccc gctcgtggga cggtcggccg accttgccgg ggcgcttgac tccataatgt
     5101 tcaacccgaa tccggccccg ttcccgtcac tgcccgccct ggtgacgtgc gccgactatg
     5161 cgtggaatcc gaccggttat cagccggaag ccgcaattga ccggatggcc gccttccttg
     5221 ccggaaagag cccggcggcc acggcactgg ttatccttgc aaggcataac cgttcccacc
     5281 tgttccacgg ttcaatggcc cctgaacttg cacccttgtt gtcgcagttc tgggtcgatt
     5341 tttccgatgg aaacgccacg ctcaattcgt cggctgtgaa gaccatcttt tccgatctgg
     5401 cggggcttcc ttcaagctgg tcggtggcgc atcttcccga acccctgttt ctgtccctcg
     5461 agtcgttcgt caatcaggcc ggccgcatgg gagtggccgg aatggatgcc gtgaatcttc
     5521 tttcctcaat cgctgccggc ctggaaccgg agccttcaat aatcgaatct ttcaatcaga
     5581 ctatggccga gattgccgac cacccggaag aaccgtctgg cgacgttatt gtcgggtttc
     5641 ttgaacaggc ttcgatggca atcaatgttt cgaattgatt ccggcggcta cttcagtccg
     5701 ccgaaggtcg cggcggtcag gttgtcttcc cacttgatgt cgaacctgga cgaaagcctg
     5761 gtcagcaggt tcatgtattc ttcatggttc agcaggccgt cctgccacgg aagcacgacc
     5821 cacttgcctg ggggcgcgaa gacaggaaga tagcgcctga cctgggtcgc cgaatccaca
     5881 cgaccggttg cggcatccac cagcgcccat tccatgaact cgtatcccct gtccatcctg
     5941 gcaatcgcga atttcttgtc gtcgaacgcg cccgaacgaa tctttccggt tgccatcagc
     6001 gcaagttcga cctgcctggg gaacttggtg aaaaccttat ggaatgacat gaccaattcg
     6061 tttgcccggt tgaaatccat ctggaacctg gaatgtcccc tggcgctgaa cgcgatcgaa
     6121 gatcccggct ggttcttgtc gaagatggtt ccttctgaaa ttccgaattc cgtgaccggg
     6181 cttccggtct tcgcctgggt gaccttgaag tccatgacga cggagccgag cgggtagaag
     6241 tgataatcgt tcgtgacggc gacggcaccg cccgaaaggg caagaaccca gaagttgctc
     6301 ttcttgtagt cggcgccgta ggcatgcttc aggatcggtg tgataacgaa gtccgcgtcg
     6361 ccctgggact ggacgacggc gcaaacgccg ctggtgttga gcgccgtctt caagcctgtc
     6421 ctgatgcttt cagggacggc ttccccaccc aggttcgtcg catcctggta aatcggtccg
     6481 tcatagctga agtaccagta aacaagaagc ggcagggcca tgttggtgcc catcttgttt
     6541 tcgccaacct tttcgtattc aggtctttcg tcggcgacgg gggccacgaa cacgcggcaa
     6601 tcgtgcgcct tggaaaccgg aatgttacgc ggcatggcct ctcgaccgat gtcgcgcacg
     6661 aaagtcggac cgcagcctgc gaacatgacg atgaacaggc agcctgcaag gagcggtatc
     6721 aatgaatatc ggcacataac ggattttaca ttcatcatgg ctacctcgtg ccggcaagct
     6781 tgccggcggt ttcaacggcc attatcgacg ccacggtaaa gagctcgtca gccgattcgg
     6841 ccgaaacgaa gtcctgggag ataacctctg acgaatacag ggctttgcct gtgcaatcct
     6901 tccaggcgta tgaatatgtg aaggcaacga gatcgaccag cttgaagcct tcatccgctt
     6961 tctgcttcca tctgtggagc tcggcatgat ggattgtgag ttccaggatt ctcagtgtct
     7021 gcgcttcgcc cccggcgcac ttcgacgggg cttccgaaga gtacatcctg aaggttggaa
     7081 tgcccgccgc ctggtactgc tcgacgaagt aatcgaaaac caccgcggac atgtgctcgt
     7141 tgaagtacat ctcgcttgtt tcggtaatgt gggattcctc gtcactcaaa tagacgccgg
     7201 tcggggcaag aatcgtgtcg gcaaacaggc ggaccaccac ggttggcggc gagtcggggg
     7261 cctggagccg gctggcctcg cggaacgaga ccttgtcctc gtcaccggcg gcggcaaccg
     7321 cgttttccca gatcttcatc ggttgaaggt gcagcattcc atatttcggg gtacaagcca
     7381 gaagcatggt tgcaaagagc agcatggctg tcaggagcag tctttcaagg gtgtttcgct
     7441 tcatcatttc acccccgggg cggcaagccc ggcaagggtc gagccgctgg aaatgttgct
     7501 ggaagtcggg ttgatgccct tcaggacatc ttcagtcacc gtctcgccgg cagacaggac
     7561 cgctggttcg agcgggctgt tgaagggggc atcgacgtac tgctggttgg cgttctggga
     7621 tgtctgggtc gtcgtcgtgg tcgtgccgcc gctgctgctg cttgaggaaa cagcgacggt
     7681 cctgaccttc aggaagcgct ggtggaacgt tccccacttg gcgccctgcg ggggattggc
     7741 cggatagatt gccttcggcc cgcacccggc aagcgcgagc agaaacagtg acaccgagga
     7801 gataagacag atactgacac atagtctgcg gacggttgtt ttatgcatct gggcaaaacc
     7861 tccctttaat tagacagttg ctgcggtata tcacacgcaa caacgttttc aatatcgatt
     7921 tctataacgg atttgggctg atttggcgtc tgtcgctgtc gtaaatgacg agccgtgaaa
     7981 gcaaggtggc tagtgcctga accgatcgaa gcccggggtc gtccgctcct tcggtgcgcc
     8041 ggaagccgga tcgcgaacga taaagaggac ttcgacgccc tccatcttcg ccgcggcctc
     8101 gatggcgcga tcgggaccca tgacggcgaa tgcggtcgca agaccatcgg cgatcgcgtt
     8161 tgggtttggc ccgccactga cgaccacggt cgttccaaga acgtcggtcg ggatcgggcg
     8221 tcccgttatc gggtcgaaga tgtgatagta cgccgtattg ccgatcatca cgggctgttc
     8281 gtagttgcca ctggtcgata cggcggttct tcccgagatg ttcagaatgg tccagttgct
     8341 gccgggctca agcgggtggc gtattccgat gcgccacggc ctgccggacg gcgaattccc
     8401 cgcaagggcg atttcaccgc cgacctgaac cagggccgac ttaaccccgt tatcctgaag
     8461 gaatgtcatt gccatgccga cggaccagcc tttgacgatg cccccgaaat caagttccat
     8521 cgcgggccgg ttccttgcaa tttcccactt gccttcgccg tcccggagca gggtcacgta
     8581 tccccatccc atacggccca gcgcgtgcgt gatatcggct tcatcaggaa ggacgccgga
     8641 ctgggcggca tttttccata tggttttcag gggccttacg gtggcgtcga aagccccgga
     8701 agtcatctgg cttacggtgg ttgcggagcc tgcgacctgt gccatccagt cgcttacctg
     8761 gacgggcgag gttgtctggt ttgagttcag gatagcgagt tccgatgacg ggtcgaaggg
     8821 attgaagaca ctgccggttc gaaccaggat atcccaggcg ccgtccagcc ttttcgcagt
     8881 cgagaggccg ccccggcgaa gatcgccatc cgggacgttc ttcaggttca tccggatggc
     8941 ctcgccggta tctgtcatga attcgatgcg ggccggaatg ccggcataaa gaaatccgct
     9001 tcgactggca acgttcgaac tgtcagaggc gcccgggtcg ccgcgaagtc cggggatcat
     9061 gtcgagcacc agaagcgcgg caacgatgac cgaagccacc gatgccgtca agaccagcag
     9121 cgccttgcgg cgcgaatatc gtgggccgtt attcccgttc atccggatca cccccggaag
     9181 tcgctgattc gcaaacggat ccgaccatcg gatcccgccc gtgcgtcgtg ttcagtcggg
     9241 ctgccagttc cggaagggtt ccctcggtga tggcatttcg aacctgttcc atcagtttcc
     9301 agtaatagtg caggttgtgc atggtgttca ggacgtttga caggatctcg cccgccacga
     9361 aaaggtggcg aagatacgag gcggaaaagt tccggcagac cggacagtcg cattcaggat
     9421 cgattggccc gagatcatcg cggtacctgg tgttcttgat gttcaggcgt ccgacgcttg
     9481 tcaggtatgt cccgttgcgt ccgcacctgg tcggaaagac gcagtcgaac atgtcgatgc
     9541 cgcccatcac gcagttcggc aggtcggccg gcatgcccac gcccatcagg tacctgggct
     9601 tgtcgaacgg gagaaccccg gctgtcgatg ccgccaccgg gtacatctgg tgcggcggct
     9661 cgccaaccga caggccgccg atggcgtatc cgtcaaaacc caacggcgta agtgccgccg
     9721 cgtgctgcct gcgtctttcg atgtccagcc cgccctggac gatggcgaag accgcctggt
     9781 tgcgggatgt cctggcgtcc tgacatcttc tggcccactt ggttgtcagt tccatcgccc
     9841 tgtccagctc gtccatccct gccgtcgatg gcgggcagac gtccagaacc atggcgatat
     9901 cggaaccaag cttctgctgg atttcgatta ccgattccgg tgtaaattca acagtgtcgc
     9961 cgttgatgtg ggacttgaag ctgaccccgt cctcgttgat tcgccggcga tcggaaaggc
    10021 tgaaaacctg aaatccgccc gagtccgtca gaattgccct gtcccagttc atgaaacgat
    10081 gaagtccgcc gtgcatcgcc acgatgtcag ggcccggacg aagtgccagg tggtatgtgt
    10141 tcgaaaggat gatgccgaaa ccggcctcgg ccacgtcccg cgggagcatt ccctttaccg
    10201 ttccaagagt cccgaccggc atgaagcagg gcgtctcaat cgttccatgt gctgtgtgca
    10261 gccgcccgcg ccgcgcaccg gtgcgggaat cggtcatcag gagttcgaat ctgaatccgc
    10321 aatcaatcat tcaatctgtt gccccgcccg ccgcgaaatg ccgggattca tcaaagaatc
    10381 agcatcgaat cgccgtagga atagaagcgg tatccacgtt ccacggccgt ccggtaagcg
    10441 tcaagtattc tttcccgtcc ggccagtgcc gataccagca gtatcagcga cgacttggga
    10501 atatggaagt ttgtaatcat cccggtgata attttgaagc gatagccggg ggttataaga
    10561 attgacgtgt ccttctgccc ggccgtgaca tgtccggaag ttccatcagc ctccagggtt
    10621 cttaccaccg tggtgccaac cgcgattatt cttcggccct catcgacggc ggcattgatt
    10681 gccctggccg cgtccgccga tatttcatag ctttccgtcc cgacgtcgtg ctgcctgacg
    10741 tcggccgatc tgaccggctt gaatgtccct ggaccaacat gaagcgtgac cgaagcggtg
    10801 cagacgcccg cttcccgaat cgtctgaagt atcgccggcg tgaagtgcag cccggcggtc
    10861 ggtgcggcga cggccccggt cctcgatgca tagacggtct ggtacctgat gcgatccaga
    10921 tcctccatgc tgccgtccgg ccgcttgatg taaggcggca ggggcatgtg gccgaatcga
    10981 tcgaaaagct cggccgccga acatgtcgcc tgggcgccgt cgcattccat cctgatatgg
    11041 gcgaccccgc ggtcggttga tgtcacaatg acccgtaatc ccgagtcatt cgaatccctg
    11101 tccagtacgg aaagcctggt tcccgctttc aggccgcgat gagttccata cattgccgag
    11161 aatccgtatt cggcggttgc aagggcaagg agttcgacga gcccgccggc cggttttctt
    11221 gcgaacaggc gtgccgggaa cacccgagtg tcgttaagca ccagaaggtc gccggggcga
    11281 agaaagccga cgatgtccga gaatacgtga tccgtcggcg ccccaccgga aatgggaaga
    11341 tgcatcagcc tggaaccgtc gcgacgttgc gccggcacct gggcgatcct gtcctgcggc
    11401 aggtcaaaat cgtacatctc aagctgaaat tccggcggta tcgtatccga caaagtgcct
    11461 ccgggcggga aatcggcccg ctgtcaatga ccgccaccgg ttcccggcct ggccttccag
    11521 attcttgtca gcaggaagac gccaaggaag gcgaaaggca gcatgaacag cggccagtat
    11581 gaccaggaac gggtcgtcag gaagccaagg ctcacgcttt gcagcgccgt tcccagatag
    11641 acgaaaccgt cgatcattcc gaccgccgtg gcggcaccct tgcgaccgcc gaagtccatc
    11701 gttgccgtgc ctgaaagaag gccgtgggtt ccgataaccc caagagagac caggaaaaga
    11761 atcagtccca tgaacaacgg ggtcaacgtc aggaccggac cggcgtccag tcgcctggcg
    11821 tcaccggcgc gtgcggtaac aagaggatcc ttcagcgtca cgctgaccgg ggcaccgtca
    11881 cgaatgacat caagctggaa ggttcctgaa gatgtcgcga tgccgtccga ggtttccctt
    11941 gggcttgcgg tgcacatgca tgcggaagtg tcccacctga cgccttcccc ctcgcatttc
    12001 gaaggaatgc acgcaaccgc cctggaaatc tgtgaccagt ccttcaggcc ggattgtccg
    12061 ttgacggaaa ccaccttgtc gcccttcatc agcgggtagt cggggcttgc ggtccagccg
    12121 accgtggtgg tggtggatcc aagcgtgaag tacatcgcca gaaggcagat ggccagcatc
    12181 acataaagcc cgcccgccgc gggcgcacgc ctgctctgga aaaacaggtc gctgacccat
    12241 ccggccacgt ttccgccgat gaccccggca acgaacagga ttccgcccca accgccctgg
    12301 atgaacgggg ccagcgcgat gagtgcgccg gaaatgatca tcgacgcctt gcgcttgccg
    12361 gtgaacctgg tcgaaaggac gaacagagcg gccgcggccg cgaacatcag cgcgataatc
    12421 caccacaggt cgccggacca gcttccgttg cgcaggatgt gcgacgaagg aagcgcccag
    12481 acctccttgg cgtaaatcgg gaaccagtgc atgacgccgt tgcggatgac accggtacag
    12541 agttcgatga acgcgacggt aaggatgatc gggttcgtaa gaaccttttt cacgatgaag
    12601 gaaagcttga cgtcgtcacc cattcccgat gaagcgtcac cagtatcgaa atcgtcgtga
    12661 ccggcgcctg aaggcgaatc gcgaaggagg atcagttcaa cgaagaacat tacgaagagg
    12721 atggccgaag gggccaggaa gacccaccag gtcgcctttg atccggccgg cccgagcccg
    12781 gctgtcaggt cgaggatcca tgaattaacg gtgaaggcga agaagatgcc ggacgaaatc
    12841 atcgtgccga aaatgccgga aaatccgccg cgttcacgta cgtggaacca ggccgagttg
    12901 accttgacga tggccaccgc cgcgaagctc tggaaataca tgttgatcgc atacatcacg
    12961 gacatggccg ttaccaggtt gatcgagacc gaacccggat tgacgatcat gttccatacg
    13021 aaaagcccca tgatcaggtt catcagtccg gaacccatgg aagccgccag gatggccttc
    13081 tttcccccga tcttgtcggt gagcgggccg ttcagaagga acgcgaacgc atagacaagc
    13141 gtgccggccc cgaagatgac gccaaaatcc tctttcgtca tcaggtcgcc cagggcattt
    13201 ttggcgacgg tcaggttgta tcttcccatg taaaggaagg cgtacgtcag gcccatcggg
    13261 aaccagttca ggaatcggcg tctcttgaag ctggcggaat gaatcggcat ttctgaagac
    13321 atatcggcca cccgtcttat tgctttttga acggctgaga gccggtcggt tgcttgcgcc
    13381 ccgaacccct tctgatttgt tccagttcct tttcaagccg gccccggttg agcgggttcg
    13441 gttcggtcaa aatggcattc cggagcatcc tttccgcatc atccgtccga cctttggcct
    13501 gaagacgggc ggcgctttca atggtcgcct cgaccggtct tggcccgtgt ctgacggtca
    13561 tcggatccgc atccttgcgc tgacgcggtt cggcctcggc cgcagccttt tcctggcgcc
    13621 tgttttcatt gaattcatca cgcgtcgtcg gtatcggaac ggcgaggctt cgccttgccc
    13681 ggtcgcgatc gatcgactcc atgatgcgcg gtggccgctt cctgggattg acaagtgccc
    13741 cctgcctgat ctggattggc gcggggccgt cctgatggcc tcccagcgta tccctcaagt
    13801 gattttcgga gcttccggcg gcgggggcac gttcaaggat gaagaacgcg ccggtcagga
    13861 cggctgccgc cacgaccatg gccgcagcgc cgatgatgag tttcatggtc ttgttcacga
    13921 cgcgcgcctc cttgaacgga agatggcaag aattgccaga gtcaacaggc acatagccgg
    13981 cagcagtccc gggtcggccg tcccggacgc ggaagcgcat ccaccgcctt tcacggcagt
    14041 cgtatcgtca ccttcgttac cttcatccac gtcatcaatt gccaggtcgg ccccgggccc
    14101 gatgtcggcc acgacatcac gtcccgcgtc agacacggaa acatcgttgc tgccgatttc
    14161 ttccaggaca tcgacgggcg gggaagaatc ttcgcccggc tgcgcgacat cggcggtcac
    14221 gtcatcgggc gaagtcgcat cctggcccgc gtcttcgacg atatcctcaa gtacgtccgg
    14281 cgtcggttcg acatcgtcca caatcacgat cggctcgttg tactcgtcgg aatcaagaac
    14341 cgaatcgcag ccgggatcat ccggccagtc tgtcaggccg tcctcgtcct cgtcaatgcc
    14401 gtttccgcat gtgtaggcta caacgttgaa ccgaatcctg tgaccggttg aaggaccggg
    14461 catgcacctt acttcaagcg agtgggagcc ctgcgccatg ttgcgcgtgt ccatggatgc
    14521 ctggaacaag tggtttccaa ggggtgccat cgcgtgccag ccaaggtcgt caaccctgaa
    14581 gtcacaggac aggaaggggt cggcggtgaa acccagtgcc ctgaccggcg ccgattcaag
    14641 gccggccggg acgtcgaagg cccacggatt cgattcaccc tgtcggctat ccgaaggtgt
    14701 cgtgatgatc acccatggcc attcctggat cgaaacctgg cggatgtgca ggttgtcgtt
    14761 atcgaccgaa accagcagga gctggttctc gtcggccttg ccgaacgaat cgacgttctc
    14821 gtgaagcgtc gtcggcatcc attcgttttc gggccagtag agatcgcggt attcgtgcgt
    14881 gtggccgtag aagtacgcac ggacgttgaa gtcgcccatg agatcctgga tctcgtcgct
    14941 gccttcacca agcttgaagc ccagatcggg cctgacgatc gggtgatggc cgaacatcag
    15001 ggtcaggcgg gcctgggtgt tatcctgcag cgcaccctgg atttccatca gttcccccag
    15061 atccaggccg gcgttgtcga acgggaatcc ggcgccgtcg ttcccggcgg tatcgatacc
    15121 catgaagtga taacttcccc agggcttgtc gatgacgacc gaatgctggg tgcgtccgaa
    15181 ggtcgttccg gccagggaat actgaaggta gtggtcgagt ccgcgctcgc tgtactggtc
    15241 gtggtttccc ggcaggtcga tgtatctatc gacggtgatt ccggtgtcgt caagaatctg
    15301 cctgtaaagt tgccactcct cgtcctgctg gccgggatgg acagagacaa gcggttctcc
    15361 cttgctggcg tccgtcaggt cgcccatgtt gaagatgatg tccggttcaa cgatgtccat
    15421 gcagtcggtc agaagccact cgaggcgctc gggaccgtcg gaatcccttg ccgtgatgtg
    15481 ctggtctgac atgacaagga accagaagac gcgggaatcg tcgccgggat acgccggttc
    15541 ggcgggctcg gcgatggaag tcgtggaaat catcagaaca agcgcgaaag gtacaagcat
    15601 cttcattgct aaaagacctc ggtggaacgg gggtcaagtc cggctcaagc cggccgcggc
    15661 ccttcccgga tcccaacgcc gccggaactt gcctgatcgc cgcacgggcc gccggccaac
    15721 gaactttacc agaaaatcca cgtttgtggg ccagacaaaa caggggtgcc acggggaatc
    15781 attgcccgtc agaccatgaa gtgaatatgg ccccgatggc ggccgcggga ttgtccggat
    15841 ccggaaggat gacctggaag cggccgtttg aatcggtgat tgcccgtcca gcttccctta
    15901 tcgttcggga tattgatgat gtcttcatcg gccattgtcc gtactgttcg aaggtcgtcg
    15961 gaaagaagaa cgtaatcaag gctttttcaa ggggtttgcc gtccgccgac ctgacggttc
    16021 cctcaagcat ccttccggag gttacggaaa tctggatgtt ttccgtgcca gtgatggtca
    16081 ccaaccgcca ggacgcgggt gcgagcccgc ttccggaaaa gggctcaacc ctgaccgcat
    16141 aatttccagg atccaggcga acaaggaatt cgccacggct gtcgctcatg gcctcgtgaa
    16201 ccacgtccgc gtcggtcggg gtttccaggg gcagcagttc ggccgcggtc gatgcgaaaa
    16261 caatcctggc atcccgaatc ggatcatcgg ttcccgaata aacgatacgc cccgaaatcc
    16321 tggggcgggg cggtagttcg acggcgaagt ttccggactg gccatcctgg aaactgaacg
    16381 cccgctggcc catcgagtac tgggccgcgc gaagccttga agaaagcccg tagccagggg
    16441 ttaccgcgtc aatcctgtaa tcgccgggcg gaagcttcag cagtttgacg cttgtctcgt
    16501 caacggtcgt gtgcatccgg tatgtcccgt cggggccatc cccatggatg aacagcctgg
    16561 ttccggcgac cggcaccctg acggcggttt ccctgagtcc gacgacataa agagaaatcg
    16621 aggccaccac cggcagactg ccgacgtcga gcacaaggaa gtcggtcgaa gggagcgcct
    16681 ggcggaagtc gtcgatcgag tcccacttga attccctgtc ggcgaacacc ttgccggact
    16741 cgccgggcgc cacgacaatc cggatctgtc cgggaagatt cggcaccgat acatcaaaga
    16801 caccgaggtc gtcggttaca gaggaagaaa ccagctttcc gtcaatcagg gcggtgacga
    16861 tcgaattccc gaccggaaac ttcccgccgt cttcagagcc gacgacgatt cccctgacct
    16921 tcgagatgga cgatttcggc ggcagatcga actgcacgct gccggaaagc ccgatgttaa
    16981 gccggaagtg ccagggcggc accgtccgct tgtcgggaac gaaggtgacc agatagtcaa
    17041 ggtcgggaat aagggccagt tcccaggcga acgcattctt ttcgtcgagg tggcccgatt
    17101 cgcacctgaa ctgaaggcct gggataagtc cttccgcggt ggcgaccagg cgtcccggat
    17161 cgggggcgat ttcgattcct tcgtcttcct cctggcgccc aagtcgtccc tgggcaatcg
    17221 ccgccggaac aagttgaagg tcaagtgttc ccggagccga catgtaaatt ccgttgaact
    17281 ggtcgccggg cgtgtcgagt cggccgttcg gctggacgga aaaggtaagg gcggagccag
    17341 ccgggtatcg cgtcacgcag atgccagcgg ccttgaagca cccctgcatc ggctcgcagt
    17401 cgatgtccga acggcatgac gccggccagg ggggcccatc ccacgtggaa tcaaacgagc
    17461 cgccgcaggc cgttcccatg agagtgccgg caaacagcag aagcggcaat gacagccggg
    17521 tcatcatgga caggacacct tcagccgaat cggccagctt acataaatat atgaatcggg
    17581 aacaaccctg ccggtaacag gatcgaaatc catcggttgc tcggttatga agagttccat
    17641 gctgtgcgtc gagccgaggg gggccagcac atcagcgaag ccgcaagggt tgaactggat
    17701 cgtgtcgaaa gactcgaagg cgggggggcg tggcgttgcc gattcgggat atcccacgaa
    17761 ccactggaag aatagttgat ccccggcatc gacattggtc actgccgaag cgaccgaaaa
    17821 ctcgatcgtg ccggaggtga gttcgacaac ctgatcgggg gacgggctga tcatcgatcg
    17881 gtcgagcgac ggcggattca ggccgtcctg cctgacgttg atttccggcg ggatggcgca
    17941 ggccagtgtt gcaagtggca gaatcagccc aagaatcgag gttcttccga tttcttccga
    18001 agcccggttc atctgcactt ccccgcggcc tgctcagccg cgcttatcgt cctgggtctt
    18061 ttccaggtac cgaaaaatcg tccttgcgtc cacgccaagc gcccttgccg tcgcggcctt
    18121 gttgtccccg tgcctctgaa gggccgcaag aatgtaatcg cgttcgaagt tctcgcgcgc
    18181 ggcggcaagc ggaaggagtt cggccgtctg ggaacccttt tcaaattcga gcgattctgc
    18241 gtccgcgacc tggccgtcgg agaaaaggac ggccttcctg attcggtttt ccatttcccg
    18301 tatgttaccg ggccagttcc aggccctgat cgcggcaagc gcctctggcg agaagcgggt
    18361 aacctgcgtc ttgaattccg ccgagaaccg tttcaggaag tactgtgcca gcgtgtcgat
    18421 gtcggcgccg cggtctcgaa gcgggggaag tttgatgacg accccggcga tccggtaata
    18481 aagatcctcg cgaaacccgg atgcctggat agcgccctca agatcccggt ttgtcgcggc
    18541 cacaaggcgg atatcgacct gatccggctt cagggcgccg accttcatta ccattccgtc
    18601 ctgcagcacc cgaagcagct tgacctgcat gtgaaaggga agatccccga tttcatcaag
    18661 gaacagcgtt ccgcccgagg ctgcctggaa ctttccggcg gtcgtcgtca ccgcgccggt
    18721 gaaagcgccc tttacatggc cgaacagctc gctttcgata aggttttccg ggattgcgcc
    18781 gcagttaatc gcgatgaatg ggccgcggct tcggttgctt ctggcgtgga gttcccttgc
    18841 aataagttcc tttccggtac cggtctcgcc ggtaaccagg acgggaacct cggtgggagc
    18901 gattctggta acccgctcga ataccagttg cattcccggg caactgccga taagtgaacc
    18961 gaaacgtgaa accttgactt cctcgcgaag agcgtcccgg tcgcgtcgca ggttgttgat
    19021 cagcattacg ctgtttacga tcgatgctgc cagcgcggca tacacgttca ggattttcag
    19081 ggtgacgtca tcgaaaaggt cggcaagcct ctcggttcca aggtaaagaa ccccaagaat
    19141 ttcccccgcg gatttcagcg ggatacacat cacggatgtc agcttcaggt tgatcacgct
    19201 ggtcgcggcg gaaaactcct tgttcgagcc ggcgtccgcc acaagttgtg attctccgtt
    19261 ctcaagtgcc ttccttacga tgctttccga aaagagaacc gcgtcatccg gaagctcatg
    19321 catcccgacg ttcctggcaa ccttgaccac cggcgatccg gattccatga agatgatgaa
    19381 gcccttggtg gcccggctga tcgacagcgt ctcgtcaagc agcagttcaa ggcaacgatc
    19441 cagatccgtc gtttccatca ggcgcctgcc cattgccgac atggcctcga gcgccttgaa
    19501 agattcgcct tcgcgtccgg acgtatcctt gaaactgaag tcgtccggca catagatcat
    19561 cgttgcggtc ccgatccgga tgacatccca ttcctggaga accgtcatcc gggtcttctg
    19621 gccgttgacc tggatttcag ccttgggatc catcggcatg atgcggtgac cctggccgtc
    19681 gagcacgact tcggcatgaa tctgcttcag gccgggaata ggcagggaga tgtcgcaacc
    19741 ttcgtcgcga ccgatcatgg tggaacgccg gaacaatgga tattccttat ggggacgtgc
    19801 tgatgccagt acgacgattc ttgccatgtc tattccgtca gcgcctccag tggaagcaga
    19861 ttccaatccc gtccggagcg ccgcttctgg actgcccggt cggacagggg atgactttca
    19921 ggttacgttt ttccgggacg aaatccttga aggcgtccgc tatgccccag attgccaggc
    19981 cgacgaaggc gccgaggctt acatactgga caatacgtaa ttttttcgcg gtgacggcgt
    20041 cggccgccga gtagaaccca tcgtcgccgc gaagcgattc ggcgccggcc cacgcgccga
    20101 tgttaagacc cagaaagacc agttcacccg taaggaagag aacgcctttg gtatttttgc
    20161 cgttcacgaa ctgtccgacg ccgaacggca ccagcatcgg gagtttcgaa cgcttctgga
    20221 cttcctcgac agtcaccgtg cactcgggct tggcctcggc ccgggccttt tccgggtcga
    20281 gtatgtgaag ttcgatcaag cggagtttca gtgtctggaa ctgactgatc aattcgccgg
    20341 gatagaagaa cggatcaagt tcatgcaatg gcgcctgggt aagaagggcc gtgaactctt
    20401 cttccatgcg ggccttgttt cccagccaga aatggcaggc ggcaaggtac tcgcgggcct
    20461 tcaactgctg gttgatgtcc tggacgcgga ttttcgggta caggagtgtt tcaagcatct
    20521 tcgcggccgc gggataatcc tggaacttga aagtgttttc tgcctcagcg aactcctcct
    20581 cgacggtggc ggcggccgcg gacatggaaa agccggccga gacaagcagg agaagcattg
    20641 tcgttaggta tcgacgcgcc atgcaggcac cggaatcagt ttcggcggca agtataacat
    20701 ttgtgggtta aaatgcgggc agtgttggaa accgggggtt cagatgtctt cctggataag
    20761 gtattgcatt tccgtgatgg cggttgcggc gtttgtcttg tcggctcggg cacagccttc
    20821 gccgaaggtc ccggatccgg ccgcggcccc ggcggtggat gccgtgtcgc ctgcagccga
    20881 agcaggcgtt gcaccgggcg ctcccgtcac cgaggcggtc agcgaacctg cggccgggaa
    20941 tgatacgcca gccgggcgtg ccgccgagcc ggccgccgag cctgcggttg ccgcttcggc
    21001 acctgtcacc gctgcggaag ccgcgccaga gccttctcct ccgcccccgc cgccaccacc
    21061 gctgatccct ggcgagtcgg tcgatgagct tgcatcgtcc atccagggtc tgtgtgaaaa
    21121 aatttcgtcc tttgaagatg tgatgaagct ggatctgtac gccagaaccg ccatctttga
    21181 ccggcttcgc atgggccttt accgggagac gctgaacctg gtctttgcct atcttccgtg
    21241 cttttccggc gcgggcaggc cgaccgccgg aatggaggat tacgaatcaa gccttcgcga
    21301 cctggcctgt tcgatgccag gcatgatttt tctgaacgaa ggttctgttg atgcccaact
    21361 tgccaggcgg tcgcttgatg catgtccgcc gccgcctccg tcacccgggc gcggggccga
    21421 cgccgaggcc gaatgggccg gcgccgtcag gcgctatctt tccctggctg ctgccgtgct
    21481 gccgccgccc gaacttctga gggttggcgc ggcctgggtt gaatcgatgg gccgaatctg
    21541 ggaaagcccg ctgcccttga agaacgcccc gacggcggtt gactggcgtc cggttgatct
    21601 gaagcatgcg atccgctcca ccgaggccct tctggtcaaa tcgactcagc ccggtggggc
    21661 gccggcttcg cttccgtccg cgaccgatga ccttggtctt tacgtcgccc caggtcccga
    21721 agttccatcc gatgtcgccg aggcaaggct tgtacttgtg gttgccgcgc tggcgaccgg
    21781 cgattccgac gcggcggtca gggccggtgg cggtgccgac atcggatgcc gatacggaat
    21841 gaacgcgctg ctgggcatcc ttgaacctga gcgtggttcc tcgcccggat tcctgtccat
    21901 ggtactgacg ccgattcttg cctccgcccc ggtcgataat ctcgtcaagg ccgtcaagac
    21961 cgcttccaac aagctgggat ccaggtgtgg aatggtttca ctgcttgcgg aactggcgat
    22021 cgggacgatg acgtttgaaa gcctgccgac ggacccggca gcgattgcac gacttgcctc
    22081 ggtcgttgcc gccgcgccac ccggtcgtcc tctgaccggc gatccggcgg tgcgggtgga
    22141 attgctgcag caggtggtgg ccagccttga tgccggcacc gatgccaggg gcttcaggct
    22201 gtatgagctt ggtcgggccc tgctggactc cggccgcccg gacgatgcgg atgacgcatt
    22261 cacgcgggcc cggcgcgacc tgccggaagt cgcccgtccg ctggcttacc agggcaaact
    22321 gctggccgct ctcgtaaggt cttcggccgt tcccggcgac gtcgtccttg ccgcaagaaa
    22381 tcttcacgac gccggccctt cgggtgaatc cctggaagcc ctggtcgccg acattccagt
    22441 cggggaaaac aggaatgccg ccattgaagc cctgtggaag ggggccgtgg catccggggg
    22501 cgacgccccg gcaatgatgg tggatctctt cattcacctg ctggacacct tccccggaag
    22561 tgccgaggcc gcgctggcca tttccctgat ggagcgtgag ggcgtcgatg attccgagcc
    22621 cgaggccttc gccggctggc ttctgttgaa gggccggcac caggtggtgg ccggggatct
    22681 gaagggtggg ctggctaccc tggatcaggc tctgaagctg ctgaagggcg atgtcccggt
    22741 ggcgcgtgaa aaggccctgc caaggctggt cttctggctt gtcgaaagcg gc
//
LOCUS       DAOPPA010000004        19343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_3451_length_19343_cov_4.583368, whole genome shotgun sequence.
ACCESSION   DAOPPA010000004 DAOPPA010000000
VERSION     DAOPPA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_19343_cov_4.583368"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PKH54_00315"
     CDS             <1..986
                     /locus_tag="PKH54_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98357.1"
                     /translation="SIYDFVNPIVFGVGAALSTLRVNSSDVEVRGGILQFTPRSGVWA
                     SQPAPSTETRAVLWFDAANSKWKTEIEPFEASPGEVAAVHQANMEAIQEGLAAGHFFK
                     ALRESVARFKAVELRPGSRQRPVVGIVGDVYTRVNEHSNDHLYRRLNDLGFEVWTSCS
                     LIDASLLGGEQLHQELLRKGKKVSAVAAMAATPAAKAALAMVDRHFPDGIRTPQERHF
                     KEVHQASSKYVSYWIDKALSMNINRIQEFHQAGADGVINAMCHNCMLGNVTASLSASI
                     RRDTDSMPICNLVFEGLKSTHSTNRLEAFAHQVMAGREARNPRSGAGIKDD"
     gene            992..2830
                     /gene="mnmG"
                     /locus_tag="PKH54_00320"
     CDS             992..2830
                     /gene="mnmG"
                     /locus_tag="PKH54_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOC98358.1"
                     /translation="MDDVFDVVVAGGGHAGVEAALAAARVGVRACLVSQQVEALGRLS
                     CNPSIGGIGKSHLVREIDALGGLMGIAADATAIQYRTLNTQKGPAVQATRVQCDRRLY
                     NSFVRDFVLSSPGVTVVQGDAVDVIVREGAAVGLRLSDGREIHGSAVVLSCGTFLDGL
                     LHFGQEKVAGGRVGEAASYGLSDSLRGLGLSVGRLKTGTPPRLLRDSLDFSKMEVQES
                     EDSAIFLSARTVESPLPKIPCHITWTTSAAHDAIRGNLHRAPLFSGQIRGRGPRYCPS
                     IEDKIVRFAHHDRHQVFVEPEGLDSPEVYPAGLATSLPLDVQKEFVRRIPGFENAVIS
                     QSGYAVEYDFVDPRECRATLQVRSVPGLFLAGQVLGTTGYEEAGALGLLAGANAALYV
                     RGLEPMVPSRARAYLGVMVDDLTTRGVTEPYRMFTSRAEFRLSLREDNAAERMLGDAE
                     RTGLVDTAHVECLKRRAARICDGIAVMDSTRVKPGQATEDVPVSSDGAVLAQLARRPN
                     ASWDSLVALDPTGRLAGLSPDEARAVLIRVRYEGYIAREQVEAERMERHDSMRIPPDI
                     EYGSMPGLSIELRGRLSRERPATIGAANRLEGMTPAALAAILVRIR"
     gene            2961..4568
                     /locus_tag="PKH54_00325"
     CDS             2961..4568
                     /locus_tag="PKH54_00325"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOC98359.1"
                     /translation="MSRLFSFLSVFVAVSVLAVAGFAQTVKLVSADTDTAKYRIDGAA
                     GIEQEGAVATAVEAALNFAASEVTGDPNEKLSAQAYVKKNLAQLNSFATPGQIFKRGF
                     DASGDKMMVGMFVMVDIKGLKAHLVAAGVTTTGAQLSDAVGKPKLLVVYNQGDCARGS
                     ETSPICAIPAKIKEFNAEVEKNEAKIDQLFEKVTASDCLRPTELRASSKTTDKAEGSA
                     SVSARRTAVGGGGMFHSWGASRTSVRASASFKRSTTTEAAMELIQASPNCKVFIDQAG
                     GLLRENQAIKAKRDQAQDELERLRAQVMDSDTTTLRVNKWLIGEQWDIVDADAINKAQ
                     RSLDAMTSTEGIPADPVAATALLAGADVYVVHDFKETSPGGAYQLVFSVKAYDVVTGK
                     LLASTVESSNKMFNEEKENATAKAIGKAMPVVINQILSYWSSMAEKGVPTKIVLRGQF
                     TNDIVDTIEEFLDERLVDVIGKSDCMGSCKWNSEMTTKQTISGTYVLPASKRQSMLRK
                     LRRSLSDEGLEYDIVVQSPTLAILQIL"
     gene            4665..6218
                     /locus_tag="PKH54_00330"
     CDS             4665..6218
                     /locus_tag="PKH54_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98360.1"
                     /translation="MFKKFWLAMILCLIVPVGAMAQEATDDVEFDKYSTTTEQLLMLK
                     GVLNTTLPAPLVDKFFFIHGFNRFKTAITESDPNTAAVQQWCMTAVAGTQTRRGALDQ
                     NMEVEITQRAVEERCSDVFKYDADVIHNCVRKTTEEGKPYLACTLNSKITFSKFKAMA
                     ANGKVSWVPDTTFGKGGKVEVKSSQRASASLDKGTTVEQATASALNTLVPMTAIIGKR
                     KMLDVQQFQVHAPVISAKKGVAKSCLGRDTVELDLPFIVMVNAGGKEVRKGFVKARKI
                     YDGCDETPEILEQAAKSKRKPEIKPMEAQIILGGKDVKAGHTMWEMPSIGLNVGGGLS
                     FSQAMGGVGSGTFKMWAAPAVNAEFNLAPYTGISEFHVWGSLKFSGFADAYLNNEFDK
                     LVSSLTSESVGSGDVTFMFQFDAGVLKRWYSRFFIFGLMGGINLGYYASLANLGDYSV
                     GALGVGLSGQIELGFQLAPRFILDFRFGYRFEAGLAMLSDGTDAVGDPKFIGVFHGPV
                     ATLNFAYNF"
     gene            6280..7761
                     /locus_tag="PKH54_00335"
     CDS             6280..7761
                     /locus_tag="PKH54_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOC98361.1"
                     /translation="MNSRHIFVLACGVAAMSISTGARASIKQVAEAVDCANRCVAAIS
                     GAVGCEAGLEACGNALWAALAKDGAATRDGAGSVQGPAKGGARPAWLDSPPVRDGFLY
                     GVGSGRDMVAAFSRAAAVISAQISVEVDSSFNMVTAVREHQIESRGRVRGSTEAIQLT
                     QETLSTRTGGVLTGVTVAQSWEDVSGEVSVLAVFDFAAFKQDKQRAIEQLGIKIFDAA
                     WDIAATIQAAGRIGPSELAMMNSYLLYLKERIRDTDDPDNVWRKAHIEFQAAIAGILG
                     CISPSNLDFEGPLLVSARVNRLAFSCNGRPWDQTRFALEPVGVLLDGPDQVVTASDGT
                     TTAKVGKIYGSGTEARVSHAGLDKLPILKGLWRKSGVAGRLELSGQRLPGSTVVTVEG
                     VGKDQAGLKDALGAFVARHWGGEAAENDGQLQMKATVSLGQAVAVGGKQNVPVVMEIE
                     VREAATKKVLLKEQVKSAALGESVKEAGEAAIKNLAPLIRKMK"
     gene            complement(7763..8176)
                     /locus_tag="PKH54_00340"
     CDS             complement(7763..8176)
                     /locus_tag="PKH54_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOC98362.1"
                     /translation="MKITRATEYACRALRYLNTRIDDRCYSIQEIAEAEEVPVQFLAK
                     VMQSLTQAGIVRSTCGKSGGYRIRRDPSEITLADVFTAVEGPFALNPCLDEDTPCSLR
                     EKCRVHDVWKELQDAMLEVLNKYTVNDVAPKQEDC"
     gene            8424..11117
                     /locus_tag="PKH54_00345"
     CDS             8424..11117
                     /locus_tag="PKH54_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98363.1"
                     /translation="MKSRFCFQVMLLAVLLSSPVVAQVDSARYGEFKDRKATVDTEIA
                     SQVTAMEQQFTVDSAPLVAKKAEEQLRLDQTTRQWNDMTRAGFENKVFNIILDIVKSK
                     VSIPFFYLTILLALAYLFFLKRALFMRHRILISVIAGILLVLYSVDLFAQDNSSRRLI
                     DSKITTVNRLLNAGEIDKAIVSIEETSETFIEMSLPDVSSTWLVPVESFRKGSFTEKY
                     TLGCLYYENKDFNKVTELFSGALSLLKPTKGELDQVVAMLKYFEEIKDPVNALLAIGK
                     ASAIGPEQGDLLAHLDTAGLGPEELRATLEMLLSRMRGAVDFLKAAVYLNSHGDPARA
                     SEMYARAVRTARDSAGYLGLADYAYNNGLPAEAAGAMDKAFKSARTTEAFLAYFKLAT
                     KHQIPNAPEAWDRAVASARSIADFVLVSRYLKEQDKLSDAIAYLKMSVDKKGTLKDLL
                     DVMNLAAEMNSAALVEQIVDSVLARTRRFEDFMKIAGVCNQVAPGKLPQVFSSAVASA
                     YKLSHFRALAGFVVGASDKGLISVVVSGAVQKIGGARDLLTLRKELMEKGFKDAVGPL
                     NMAVVNRLRFHNELMSLFNMFEAEGFRDAAEMALARIVAVSSKQNVIENIMNMALERE
                     YFSAAFDGCLRLLPSRDSTNVPDPRLIPEASLVPNGPEIEYGVFCAVIGHKAGRSADA
                     LAVLETRVAGFLEAYINDPDSVIAGPVNTYFYLRLLWELSGVSSMVPRYEQIYKVVET
                     RYLEEYKAEKSEAISEKQIELENRRIALNQEIAAIQAETEETAQKIEAFREEHRKQVF
                     KKIASFMRVSATVGLFIVGIVIAAIAGWRFSRKLTRFRISGFTFKFFETFGFELMFTI
                     ILLPVGLVMLVVSQVSMMVQHIQQDAEEEMLCDRVKGYDPE"
     gene            complement(11190..12650)
                     /gene="guaB"
                     /locus_tag="PKH54_00350"
     CDS             complement(11190..12650)
                     /gene="guaB"
                     /locus_tag="PKH54_00350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOC98364.1"
                     /translation="MRMLIDKPALTFDDLLLVPQHSQILPKDAILKTRLSRRIELNIP
                     LVSAAMDTVTESGTAIALALEGGIGVIHKNLSIADQAREVRKVKKAESRIIDDPVTVT
                     PDLTLAEVRQLMAKRGYSGFPVVDSEGRLVGMLTNRDVRFETQMNKLVSDVMTKTPVI
                     GRAGISYADAREILHREKIEKLPLIDENHKLVGLITFRDIENVGKHPNAAKDHRGRLL
                     CAAAIGVGPDRLDRAAALVEAGVDLLALDTAHGHSAGVIRAVAEIKARFPDVDVSAGN
                     IATAEAVKALVDAGADVVKVGIGPGSICTTRVVAGIGVPQATAVAMCAEEAQRHDIPV
                     IADGGIKFSGDLVKAMALGASSVMVGGMLAGTEECPGDVVYYQGRSYKVYRGMGSLSA
                     MKSGSKDRYFQDDYEPEKLVPEGVEGRVPYKGPLSKVVYQLMGGLKAGMGYCGARTIP
                     ELWERATFVQITDSGLRESHVHDVQITEEPPNYNTR"
     gene            13033..14310
                     /locus_tag="PKH54_00355"
     CDS             13033..14310
                     /locus_tag="PKH54_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOC98365.1"
                     /translation="MRPRTLDSLVGQQDVLGEGGFLREAIEADIIPSVILWGPPGCGK
                     TSLARIIAERTMSVFVPFSAVMSGVREVRAIAASAVERLQSGGRRTILFIDEIHRFNK
                     AQQDAFLPHVESGAITLVGATTENPYFEVNSALLSRCRVVRMAPLSEQEVLLLARRAL
                     GDPVNGLGAHDISISDEMLLALARASDGDGRRMLNLLEACFGHAVRRGSRVVDQAVIQ
                     AVLTGPGLRYDRASEEHYNVISAFIKSLRGSDPDAAMYYMVRMLEGGEDPRFICRRMI
                     IFASEDIGNADPRALAVACDAKDALLFLGLPEAEIPMAQACTYLACAPKSNRSYLALH
                     AAKDAVKNTGSLEVPMHIRNAPVKAMASHGYGQGYAYPHDYDDAFVSCDYLPDALAGS
                     RFYQPRDCGYEKTMGERLEWWRSKRRPDPPRDR"
     gene            complement(14523..15893)
                     /gene="murD"
                     /locus_tag="PKH54_00360"
     CDS             complement(14523..15893)
                     /gene="murD"
                     /locus_tag="PKH54_00360"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOC98366.1"
                     /translation="MLVEGRKFCVIGMGKSGIAASNLLASHGAQVVLSDMRDNRDLRE
                     SVAGSVDRRVETVFGCEVVRPGSVAVLSPGIAPHAPAFRLAQRVADEVIGEVELFFRF
                     FPGRTIAVTGTDGKSTVTTLTAHLLNACGIPARAAGNLGNALCGEVQDLTEKDVVVAE
                     VSCFQLITCRQFRPEVALVTNLAPDHIDHHGTWEAYVRAKAMVGAAQMAGDAFVRNID
                     DPVLKTWFTPGNRWISGNGQAVVDVSLEHSVEDGAFLEDGGLWIASRKRAIEVCRRDE
                     LKLPGPHNTENALMAIAACMALQDRRVSIPSLVNGLRTYAGLPHRIEFVRHLDGVDWY
                     NDSKATNPHAAAVGIRSFAGRPLVLLAGGYEKNLELKDMATAIGERCRHVVLFGACAE
                     RMKREFPKAVPLEIVQDMRSAVNRAREIADPDSVVLLSPGASSFDQFTSFEDRGDRFK
                     KMINEM"
     gene            complement(15915..17306)
                     /gene="murC"
                     /locus_tag="PKH54_00365"
     CDS             complement(15915..17306)
                     /gene="murC"
                     /locus_tag="PKH54_00365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOC98367.1"
                     /translation="MSQIELNDFKHFHFIGIGGVGVSAVARQVLHAGHKVSGSDVRES
                     CLTIEMRNAGADVFIGHNPENLRGADLVVVSTAIPETNQELLAARKAGIPVVHRSMIL
                     DAVMRGRKSIGVTGTNGKGSVSAMITWLLECAGVNPAFAIGARLLNFQTNSRPTDGET
                     IVCELDESDGSFTNIRPDVMVINNLEADHLNYYKDLEGLLALFSGYLAGPMAPRRLHA
                     NGDDENIAEVRRRSGFPGVTFGCGPSCDFVVRDIRTVGMTTVFSMSGPDGDMGEFILH
                     IPGEYNAMNMAGAISVAIHEGVPLETVRAAVGTFRGLENRFSCVRTDRGLVVKDYISH
                     PTGIRCVINTARKFATGRILCVFKPYRFTMIHYLGDEYSEAFKGADLTFVTRMYTAGE
                     VPIPGIDTDWLVGRIRDAGSDVAYIPEMTDIIPEILKVLRDGDTIIFFGGDDLFQLAD
                     ELVQRLGAVSSAG"
     gene            complement(17462..18328)
                     /locus_tag="PKH54_00370"
     CDS             complement(17462..18328)
                     /locus_tag="PKH54_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98368.1"
                     /translation="MSQTKRVPMMPRRFSISAWTGRVLVAAALAAATLHGCATPQPAE
                     PPARWKVAEIQTEVESEDCCPALAAAGFALLAARDMAMPGNSLRIALGCRPAGDGIEV
                     ASMLQLKVFSRTLESETFESANSDTIFACEDGFLSLGASLFDLIRQVSEIQAISGVAD
                     EKLCEIASRRRGPVPLAVLMRALEELAVRNNTDCVAAVEELALSSSGDLALRAIGTLG
                     RLKVPRTVLTLGRLTLSNEGSIAWAATYAIADIGGPDAVRALEIIAGQSVRPTLGLEA
                     ARLAESLRERGR"
     gene            18370..>19343
                     /gene="hisS"
                     /locus_tag="PKH54_00375"
     CDS             18370..>19343
                     /gene="hisS"
                     /locus_tag="PKH54_00375"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOC98369.1"
                     /translation="MKSTTSSTESRPNKSTIEPRLLKGFRDFLPDEQAARQTMLSGIT
                     GAFELCGFAPLSTPAIEYADILLGKYGDEGEKLLYKFEDNGGRQVALRYDLTVPLARV
                     VAQYRDIPKPFRRYQLGTVWRAEKPAHGRFREFMQCDADIIGTASSLADADCIAAGIM
                     GLKSVGLERFEVRIGHRGVLNDVLGQFGVTVPDAQADILRNLDKLDKIGPGAVAETIA
                     AQGLMDADQAGRLIATISGAGVPTVVTEAGLAGAEHLRGVLQALESMGFGPFIRVDMS
                     IARGLDYYTGTVYETMLLDLPSVGSIMSGGRYDCLLETYGMPEVPAVG"
BASE COUNT         3753 a         5759 c         5936 g         3895 t
ORIGIN      
        1 actcaatata cgacttcgtc aatccgattg tttttggggt gggggctgcc ctttccacat
       61 tgcgcgtcaa ttcctcagat gtagaagtgc gcggcgggat tctgcaattc acgccacgct
      121 ctggcgtgtg ggcctcgcaa ccagcgccat ccacagagac gcgcgccgtg ctgtggtttg
      181 acgcggccaa cagcaagtgg aagaccgaga ttgagccttt cgaggcgagt cccggcgagg
      241 tggccgccgt tcaccaggcg aacatggaag ccatccagga ggggctcgcg gccgggcatt
      301 ttttcaaggc cctgcgcgag tcggtcgcgc gtttcaaggc cgtcgagctt cggcccggtt
      361 caaggcagcg tcctgtggtg ggcatagtcg gcgacgtcta tacccgggtc aacgagcatt
      421 caaacgatca cctgtaccgc cgccttaacg acctcgggtt cgaggtctgg acgtcgtgtt
      481 cgctgatcga tgcgtcgctt ctgggcggcg agcagcttca ccaggaactg ctacgcaagg
      541 gcaaaaaggt ctccgctgtg gcggccatgg cggcaacccc cgcggcgaag gccgctctgg
      601 cgatggttga ccggcatttc ccggacggca tcaggactcc gcaggagcgc catttcaagg
      661 aagtccacca ggcgtcgtcg aagtatgtca gttactggat cgacaaggcg ttgtcgatga
      721 acatcaacag gatccaggaa ttccaccagg ccggcgcaga cggcgtgatc aatgccatgt
      781 gtcacaactg catgcttggc aacgtgaccg cttccctgtc cgcctccatc aggcgcgata
      841 ccgattcgat gccgatctgc aacctggtat tcgaaggctt gaaatcgacc cacagcacga
      901 accggctcga ggccttcgcg caccaggtca tggccggccg cgaggcccgc aatccgcgca
      961 gcggcgccgg aatcaaggac gattgaaagg gatggacgac gtttttgacg tggttgtggc
     1021 cggcggcggt catgccggtg tcgaggccgc tcttgccgcg gcgcgtgtcg gggtgcgtgc
     1081 ctgccttgtg tcccagcagg tcgaggccct gggcaggctt tcctgcaacc cgtcgatcgg
     1141 tggaatcggc aagagccacc tggttcgcga aatcgatgct cttggcgggt tgatgggcat
     1201 tgcggcggac gcgaccgcaa tccagtacag gacgctgaac acccagaagg gcccggcggt
     1261 tcaggccacc cgcgtccagt gcgacaggcg gctgtacaac agctttgttc gggattttgt
     1321 tctttcgagt cccggcgtta ccgtggttca gggcgatgcc gtcgatgtga ttgtcaggga
     1381 aggcgcggca gtcgggttgc gtctttcaga cggacgcgaa atccacggaa gcgcggtggt
     1441 gctgtcgtgc ggaactttcc ttgacggact gcttcacttc ggccaggaaa aggttgcggg
     1501 cggccgggtc ggcgaggccg cgtcgtacgg gctgtcggac agcctgcgcg gtctggggct
     1561 gtcggtcggc cgcctgaaga ccggcacgcc cccccggctg cttcgggatt ccctggactt
     1621 ttcgaagatg gaagtccagg aatccgagga ctccgccata tttctgtcgg caaggacggt
     1681 cgagtcgccc cttccgaaga ttccgtgcca cataacctgg acaacgtcgg ccgcgcatga
     1741 cgcgattcgc ggtaacctgc acagggcgcc tttgttttcg gggcagatca ggggccgagg
     1801 tcctcgttac tgcccatcga ttgaagacaa gattgtcagg ttcgcgcatc acgatcgcca
     1861 tcaggtcttt gtcgagccgg aaggcctgga ttcgccggaa gtctatccgg ccggcctggc
     1921 cacgtcactg ccgctggatg tccagaaaga gttcgtccgc cggattcccg gttttgaaaa
     1981 tgcggtcatc agccagtctg gatacgcggt cgagtacgat tttgtcgatc ctcgcgagtg
     2041 tcgggccacg ttgcaggttc gctcggtgcc ggggctgttc ctggccggcc aggtgctggg
     2101 aacgaccgga tacgaggagg ccggggcgct tggtctgctg gccggcgcga acgcggccct
     2161 ttacgttcgc ggccttgaac ccatggtgcc ttccagggcg cgcgcatacc tgggtgtcat
     2221 ggttgacgac ctgacgacaa ggggcgtcac cgaaccttac cggatgttca catcgcgcgc
     2281 cgagttcagg ctttccctgc gcgaggacaa cgcggccgaa aggatgcttg gcgacgcgga
     2341 aaggacggga ctggtcgata cggcgcatgt cgaatgcctg aagcggcgtg ccgcgagaat
     2401 atgtgacggc atcgctgtga tggactcaac cagggtcaag cctggccagg ccacggaaga
     2461 cgtgccggtg tccagcgacg gggccgtgct cgcccagctt gccaggcgcc cgaacgcttc
     2521 atgggattcc cttgtcgcac tggatccgac cggcaggctg gctgggcttt ccccggacga
     2581 ggcaagggca gtcctgatcc gagtaaggta cgagggctac attgcccgcg agcaggtcga
     2641 ggcggaaagg atggaacgcc atgattccat gaggattccg cccgacatcg aatacggatc
     2701 catgcccggc ctgtcgattg aactgcgcgg ccgcctttcc agagaacgcc cggcaacgat
     2761 tggtgccgca aaccgccttg aaggaatgac cccggccgcc cttgccgcca ttcttgtccg
     2821 aatcagatag accccgattg ttaagattct gtcaatttgg gttgacttgt cttgtatgta
     2881 gggatatacc gtccaccatg aatccagatt gcgattgtgt atcgttaccg cggttatttt
     2941 ccttttatcg ggggattttt atgtccaggc tcttttcgtt tctgtccgta ttcgttgcag
     3001 tttccgttct tgccgtcgcc ggtttcgccc agacggtgaa gctggtgtcg gccgacaccg
     3061 ataccgcgaa gtaccgcatt gacggcgccg ctggcatcga acaggaaggc gcggtcgcga
     3121 ccgcggtcga ggcggccctg aatttcgccg ccagtgaagt caccggggat cccaacgaga
     3181 agttgagcgc ccaggcctat gtgaagaaga acctggcgca gttgaattca tttgcgacgc
     3241 ccggccagat cttcaagcgc ggtttcgatg catccggcga caagatgatg gtcggaatgt
     3301 tcgtgatggt tgacatcaag ggactgaagg cccacctggt cgccgccggc gtgaccacga
     3361 cgggcgccca gctttccgac gccgtcggca agccgaagct tcttgtcgtc tataaccagg
     3421 gcgactgcgc ccgtggttcg gaaacgtcgc cgatctgcgc gatcccggcg aagatcaagg
     3481 aattcaacgc cgaggtcgaa aagaacgagg cgaagatcga ccagcttttc gaaaaggtca
     3541 ccgcttcgga ctgcctgcgt ccgaccgagc ttcgcgcttc ctcgaagacg accgacaagg
     3601 ccgaaggttc agccagtgtt tctgccaggc gcaccgcggt cggtggcggc ggcatgttcc
     3661 attcgtgggg cgccagccgc accagcgtca gggcatcggc aagcttcaag cgttccacga
     3721 cgaccgaagc cgccatggaa ctgatccagg ccagcccgaa ttgcaaggtc ttcatcgacc
     3781 aggcgggcgg cctgctgcgc gagaatcagg cgatcaaggc gaagagggat caggcccagg
     3841 atgaacttga aagactgcgc gcccaggtga tggacagcga cacgaccacc cttcgcgtca
     3901 acaagtggct tatcggcgag cagtgggaca tcgttgacgc cgacgcgatc aacaaggccc
     3961 agcgcagcct ggacgcgatg acctcgaccg agggaatccc ggccgacccg gttgccgcga
     4021 ccgcgctgct ggccggcgcc gacgtgtatg tggttcacga cttcaaggaa accagcccgg
     4081 gcggcgcgta tcagcttgtc ttttccgtca aggcgtacga cgtcgtgacc ggaaagctgc
     4141 tggcatcgac ggtcgagtcg tcgaacaaga tgttcaacga ggaaaaggaa aacgcgacgg
     4201 ccaaggccat cggcaaggcg atgccggtcg tcatcaacca gatactttcg tactggtcct
     4261 cgatggctga aaagggcgtc ccgacaaaga tcgtcctgcg tggacagttc accaacgaca
     4321 tcgttgacac gatcgaggaa tttcttgacg agcgcctggt cgatgtcatc ggcaagtccg
     4381 actgcatggg ttcctgcaag tggaacagcg aaatgaccac caaacagacc atttccggaa
     4441 cttacgttct gcccgcttcc aagcgtcagt cgatgcttcg caagctgcgc cgttcgttaa
     4501 gcgacgaagg gctggaatac gacatcgtcg ttcagtcgcc gacgcttgcg attcttcaga
     4561 tcctttaagg cacgaagggg gcgttccggc gggccggccc agcgggcctt gttccaacgg
     4621 gatgcataac aagtccggga tggttgtccc ggcggagggt gattatgttc aagaagttct
     4681 ggctggctat gatcctttgt ttaatcgtcc cggttggggc gatggcccag gaagcgacgg
     4741 atgacgtcga gttcgacaag tacagcacga caacagaaca gcttctgatg ctgaagggtg
     4801 ttcttaacac gacccttccg gcgccgctgg tcgataagtt cttcttcatc cacggcttca
     4861 accgtttcaa gaccgcgatc accgaaagcg atccgaacac cgccgcagtc cagcagtggt
     4921 gcatgacggc cgttgccggc acccagaccc gtcgcggcgc gcttgaccag aacatggaag
     4981 tcgaaatcac ccagcgcgcc gtcgaggagc gctgctcgga cgtcttcaag tacgacgccg
     5041 acgtcatcca caactgcgtc aggaagacga ccgaggaagg caagccctac ctggcgtgca
     5101 ccctgaattc gaagatcacg ttttcgaagt tcaaggcgat ggcggccaac ggcaaggttt
     5161 cctgggttcc cgacacgact ttcggcaagg gcggaaaggt cgaggtgaaa agcagccaga
     5221 gggcttcggc aagcctcgac aagggaacga cggtcgaaca ggcgaccgcc agtgccttaa
     5281 acacccttgt tccgatgacc gcgattatcg gcaagcgcaa gatgctcgac gtccagcagt
     5341 tccaggttca cgcgccggta atcagcgcca agaagggcgt cgcgaagtcc tgcctgggac
     5401 gcgacaccgt cgaactggac cttccgttca tcgtcatggt caacgccggc ggcaaggaag
     5461 tcaggaaggg cttcgtcaag gccagaaaga tttatgacgg atgcgacgaa accccggaga
     5521 tcctggaaca ggccgcgaag tcgaaacgga agccggaaat caagccgatg gaagcccaga
     5581 tcattcttgg cggcaaggat gtcaaggccg gccacacgat gtgggaaatg ccctcgatcg
     5641 gacttaacgt cggtggcggt ctgtcgttct cccaggcgat gggcggcgtc ggctcgggaa
     5701 ccttcaagat gtgggccgcg ccagccgtta acgcggaatt caaccttgcc ccctacaccg
     5761 gaatttccga gtttcatgtc tggggttcgt tgaagttttc cgggtttgct gatgcctatt
     5821 tgaacaatga gttcgataag ctggtcagca gccttacctc tgaaagcgtc ggttccggtg
     5881 acgtcacctt catgttccag ttcgacgccg gcgtcctgaa gcgctggtat tcgcgcttct
     5941 tcatcttcgg cctgatgggc ggtatcaacc ttggatacta cgcctcgctg gccaaccttg
     6001 gcgactacag cgtcggcgcg ctcggcgtgg gcctcagcgg ccagatcgaa ctcggcttcc
     6061 agcttgcacc gcgcttcatc ctggacttca ggttcggcta caggttcgaa gccggcctgg
     6121 ccatgctttc ggatggaacg gatgctgtcg gcgaccccaa gttcatcggc gtcttccacg
     6181 gaccggtcgc caccctgaac tttgcgtata acttctagtt gtaatcccaa tcggttaaca
     6241 ctaaactgac cccgtctgac ttacccttgg aggtccagat tgaattccag gcatattttc
     6301 gttctggcct gcggcgtcgc ggccatgtcg atttcaaccg gcgcccgggc gtcgattaag
     6361 caggttgccg aggcggttga ttgcgccaac cggtgtgttg ccgcgatttc cggggcggtc
     6421 ggctgcgagg ccggtcttga ggcttgtggt aacgccctgt gggcagctct tgcgaaggac
     6481 ggcgccgcca caagggacgg tgccggttcg gtccagggcc cggccaaagg cggtgccagg
     6541 ccggcctggc tcgacagccc gccggtcagg gacggtttcc tttacggcgt cggatccgga
     6601 cgtgacatgg tcgccgcctt ctcgcgcgcg gccgcggtca tttcggccca gataagcgtg
     6661 gaagttgatt catccttcaa catggtcacg gcggttcgtg aacatcagat cgaatccagg
     6721 ggacgcgtcc gcggttcgac cgaggcgatc cagttgaccc aggagacgct ctcgacaaga
     6781 accggcggcg ttctcaccgg cgtaaccgtg gcccagagct gggaagacgt ttccggcgaa
     6841 gtgtcggtac tggccgtgtt cgatttcgcg gcgttcaagc aggacaagca gcgcgccatc
     6901 gagcagcttg gaatcaagat attcgatgcg gcatgggaca tcgccgcgac catccaggcc
     6961 gccggccgta tcggcccatc cgaactcgcg atgatgaatt cgtatcttct gtacctgaag
     7021 gaacgcatcc gtgacacgga tgacccggac aatgtgtggc gcaaggcgca tatagagttt
     7081 caggccgcaa tcgccggaat ccttgggtgc atcagtccgt cgaatctcga tttcgaaggc
     7141 cccctgctgg tttccgcaag ggtcaacagg ctggcgtttt cctgtaacgg ccggccatgg
     7201 gatcagacgc gcttcgcgct cgaaccggtc ggcgtcctgc tggacggccc ggatcaggtc
     7261 gtcaccgcgt ccgatggcac gacgaccgcg aaggttggaa agatttacgg ctccggtacg
     7321 gaagccaggg tcagtcacgc cggactggac aagctgccga tcctgaaggg gctgtggcga
     7381 aagtccggcg ttgcgggccg ccttgaactt tccggtcagc gtcttcccgg ttcgaccgtg
     7441 gttaccgttg aaggcgtcgg aaaggatcag gccggactga aggatgcgct cggggcattt
     7501 gtcgcgcgtc actggggtgg cgaagctgcg gaaaatgacg gccagctcca gatgaaggcg
     7561 acggtttcgc tcgggcaggc ggttgccgtc ggcgggaagc agaacgtccc ggtcgtgatg
     7621 gaaatcgaag tgcgcgaggc ggccacgaag aaggtactgt tgaaggaaca ggtcaagtcc
     7681 gccgcccttg gcgaatccgt caaggaagcc ggggaagccg ccatcaaaaa ccttgccccg
     7741 ctgattcgca agatgaagtg attcagcagt cttcctgctt tggcgccacg tcgttgactg
     7801 tatatttatt caagacttca agcatcgcat cctgcagttc cttccacacg tcgtggacgc
     7861 ggcatttttc gcgaaggctg cagggcgtgt cttcatccag gcacgggttc agggcaaacg
     7921 gtccttcaac cgcggtgaag acatcggcca gcgtgatttc ggacggatcc cgtcgaatcc
     7981 ggtagccgcc gctttttccg caggtggaac ggacgattcc cgcctgggtc agggattgca
     8041 tcacctttgc caggaactga accggaactt cctcggcctc ggcaatttcc tggattgagt
     8101 aacatctgtc gtcaattctt gtgttcaggt aacgaagtgc cctgcacgcg tattcggtgg
     8161 ccctggtaat cttcattcag acacctttcg tcaatccggg gatccgatac cccagtctgg
     8221 gcgctaagtt acatcagata aactggaccg tcaatgtccg gtttcgcatc tgcattcatt
     8281 tcgggccgtt ttgcggcagg cggcaacaat ctgcccgccc gacggccgcc cgcccgatag
     8341 tggtaaacca tattcgttaa atcatcttga atgccgttgt cgaacggaat agagtgtgaa
     8401 ccaaacacaa atggaggctt ccgatgaagt cccgattctg tttccaggtg atgttgctgg
     8461 cggttcttct ttccagtccc gtggtggcgc aggtcgattc ggcgcggtac ggcgagttca
     8521 aggatcgcaa ggccacggtc gatacggaaa tcgcctcgca ggtcacggcc atggagcagc
     8581 agttcacggt cgattccgcg ccgcttgtgg caaagaaggc cgaagagcag ttgcgcctgg
     8641 atcagaccac caggcagtgg aacgacatga ccagggccgg cttcgagaac aaggttttca
     8701 acattatcct tgatatcgtg aagtcaaagg tgtccatccc gttcttctac ctgaccatcc
     8761 tcctggcgct tgcctatctg ttcttcctga agcgcgccct tttcatgcgg cacaggatcc
     8821 tgatttccgt gattgccggg atcctgctgg ttctttattc agtcgatctt tttgcccagg
     8881 acaattcaag ccgtcgcctg atcgactcca agatcacgac ggtcaacagg ttgcttaacg
     8941 cgggcgaaat cgacaaggcg atcgtgtcca tcgaggaaac atcggaaacc ttcatcgaaa
     9001 tgagccttcc cgatgtgtcc agcacgtggc tggttccggt cgagtccttt cgcaagggca
     9061 gcttcaccga aaagtacacc ctgggctgcc tttattatga aaacaaggac ttcaacaagg
     9121 tgaccgagct gttttccggg gccctttccc tgctgaagcc gaccaaaggc gaactggatc
     9181 aggttgtcgc gatgctgaag tacttcgagg aaatcaagga tccggtcaat gcgctgcttg
     9241 caatcggcaa ggcttcggcg atcggaccgg agcagggcga cctgctggcg caccttgaca
     9301 cggcaggact cggacctgag gagcttcggg cgacactgga aatgcttctt tcccggatgc
     9361 gcggggcggt cgatttcctg aaggcagcgg tctatctgaa ttcccacggc gatccggcca
     9421 gggcttcgga aatgtatgcc agggccgtca ggacggcacg cgactccgcc ggctatctgg
     9481 gccttgccga ctacgcctac aacaacggcc tgccagccga ggcagcaggc gccatggaca
     9541 aggcgtttaa gtccgcaaga acgaccgagg cgttcctggc atacttcaag ctggccacca
     9601 aacatcagat accgaatgcg cccgaggcgt gggatcgtgc cgtcgcaagc gccagatcca
     9661 tcgccgactt cgtccttgtt tccagatacc tgaaggaaca ggacaaactt tcggacgcaa
     9721 tcgcctatct gaagatgtcg gtcgataaga agggcacgct gaaggatctg ctggatgtga
     9781 tgaacctggc ggccgaaatg aacagcgccg ctctcgtcga gcagatcgtc gattccgtgc
     9841 ttgcccggac ccgtcgcttc gaggatttca tgaagattgc cggcgtatgc aaccaggtcg
     9901 cgcccggcaa gctgccacag gtcttttcat ccgcggtcgc ttccgcgtac aagctgtccc
     9961 acttcagggc gctggccgga ttcgtcgtcg gggcaagcga caaggggctc atctctgtcg
    10021 tcgtttcggg cgccgtccag aagattggcg gggcaaggga tctgctgacc ctgcgcaagg
    10081 aactgatgga aaagggattc aaggatgcgg tcgggccgct gaacatggcg gtcgtgaacc
    10141 gcctgcgatt ccacaacgaa ttgatgtcac tgttcaacat gttcgaggcc gaaggattcc
    10201 gggatgccgc cgagatggcg ctggcccgca tcgtcgcggt ttcatcgaag cagaacgtga
    10261 tcgaaaacat catgaacatg gcgctggagc gcgaatactt cagtgcggcc ttcgacggct
    10321 gcctcaggct cctgccgtca agggattcga cgaacgtgcc ggatccgcgc ctgattcccg
    10381 aagcatccct ggtcccgaac ggtccggaga ttgagtacgg cgtgttctgc gcggtcatcg
    10441 gccacaaggc cggacgttcc gccgacgccc ttgccgtcct tgaaacccgc gttgcaggct
    10501 tcctggaggc ctacatcaac gaccctgatt ctgtcatcgc gggcccggtc aacacctatt
    10561 tctacctgag gctgctgtgg gagctttccg gcgtgtcgtc gatggttccg cggtacgaac
    10621 agatctacaa ggtggtcgag accaggtacc tggaagagta caaggccgaa aagtccgagg
    10681 ccatttcgga aaagcagatc gaactggaaa accgaaggat cgccttgaat caggagattg
    10741 ccgcaatcca ggcggaaacc gaggaaaccg cgcagaagat cgaggcgttc cgcgaagagc
    10801 accgtaagca ggtgttcaag aaaatcgcat ccttcatgcg cgtgtcggcc accgtcgggc
    10861 ttttcatcgt gggaatcgtg attgccgcca tcgccggatg gcgcttcagc cggaagctga
    10921 ccaggttccg gatttcaggg ttcacgttca agttcttcga gacgttcggg tttgaactga
    10981 tgttcaccat catcctgctt ccggtaggtc tggtgatgct ggttgtgtcc caggtttcga
    11041 tgatggtgca gcacatccag caggatgcgg aagaggagat gctgtgcgac cgcgtcaagg
    11101 gttatgaccc cgaatagtct tcaggccgcc cccgtccgtc gccgataagt cgcaatcaga
    11161 aaatcaagtg gaatgcgggg tgaacgggac taccgcgtgt tgtagttcgg cggctcttcc
    11221 gtgatctgga cgtcgtggac gtgggattca cgcaggccgg aatcggtgat ctgcacgaat
    11281 gtggctcgtt cccagagctc gggaatggtt cttgcgccgc agtatcccat gccggccttc
    11341 aggccgccca tcagttgata caccaccttc gaaagcggac ccttgtaggg cacgcgccct
    11401 tcgacacctt ccggaaccag tttttccggc tcgtaatcgt cctggaagta tcgatccttc
    11461 gatcccgact tcatcgcgct caggcttccc atgccgcgat agaccttgta ggaacggccc
    11521 tggtaataga cgacgtcgcc ggggcattcc tcggtaccgg ccagcatgcc gccgaccatg
    11581 accgagcttg cgccaagcgc catcgccttg accaggtcgc ctgaaaactt gatgccgccg
    11641 tcggcaatga ccgggatgtc gtgcctctgc gcttcctcgg cgcacatcgc gaccgccgtt
    11701 gcctggggca cgccgatacc ggccacgacg cgtgtcgtgc agatggagcc cggcccgatt
    11761 ccgaccttga ccacgtcggc cccggcatcg acaagtgcct tgaccgcttc ggcggtggca
    11821 atgttgccgg cgcttacgtc aacgtcgggg aacctggcct tgatttcggc aaccgccctg
    11881 atgacgccgg ccgaatggcc gtgggcggta tcaagcgcca gaagatcaac accggcttcg
    11941 accagggccg ccgcgcggtc cagccgatcc ggtccgacgc cgattgcggc cgcgcacagc
    12001 agccgtccac ggtgatcctt ggcggcgttc gggtgcttgc cgacattctc tatgtcgcgg
    12061 aaggtgatca ggccgacaag cttgtggttt tcatcgatca acggcagctt ttcgatcttt
    12121 tcgcggtgaa gaatctccct ggcatcggcg taggaaatac cggccctgcc gatgacgggc
    12181 gttttcgtca tcacgtccga aaccagcttg ttcatctggg tttcaaacct gacatcgcga
    12241 ttggtcagca tgccgaccag gcggccttcg gaatcgacga ccgggaagcc ggaatacccg
    12301 cgcttcgcca tgagctggcg aacctccgcc agggtcagat ccggcgtcac ggtgaccggg
    12361 tcgtcgataa tcctggactc ggcctttttc accttgcgga cttcccgggc ctgatccgca
    12421 atcgaaaggt tcttgtgaat cacgccgatt ccgccttcca gggcaagcgc gatggccgtt
    12481 ccggattcgg tcactgtgtc catcgcggcg ctgacaagcg ggatgttcaa ttcgatgcgg
    12541 cgggacagcc ttgtcttcag gatggcatcc ttcggcagga tctggctgtg ctgcggtacc
    12601 agcagcaggt catcgaaggt aagggccggt ttgtcgatca acatgcgcat gatgtgtact
    12661 cctgcccctt ccgtcgggga cacgaccaaa cttgcttctt tgaaccgatg cttcccggaa
    12721 agttgcttga caggtgcggc ctccggggcc accgggtatc catttgtatt agaatagcgg
    12781 ccggttgtaa aggggcattc aatgccgaac cgggaatctg gaatcgagga aggcgacgac
    12841 ggaaccatcg acgacggccg tgatctgttc ggcgagccgt tgcggcaacc aaccgagggc
    12901 gcgcgtggag gggggcggcg ccaggatcgg gcggcccatg aaaccgaagc agcctggcag
    12961 ggatccgccc ttcgcggcga ggattcgggt gatgccggga catccggcgc ggacgcgccg
    13021 ctggcggatc gcatgaggcc ccggaccctg gattcccttg tgggacagca ggatgtgctg
    13081 ggcgaaggtg gtttcctgcg cgaggccatc gaggcggata tcatcccgtc cgttatcctg
    13141 tggggaccgc ccggatgcgg caagacttcg ctggcgagaa tcatcgcgga aaggacgatg
    13201 tctgttttcg tgcccttttc ggcggtgatg tcgggcgtgc gcgaggtacg cgccattgcg
    13261 gcatccgccg tcgagaggct ccagtcaggc ggccgcagga ccatcctgtt catcgacgaa
    13321 atccaccggt tcaacaaggc ccagcaggat gccttccttc ctcatgtcga gtcgggcgcc
    13381 atcacgctgg tcggagcgac caccgaaaat ccgtatttcg aggtcaattc ggcgcttctt
    13441 tccaggtgtc gcgtcgtcag gatggcgccg ttgtccgaac aggaagtgct gctgcttgcc
    13501 aggcgcgcgc ttggcgatcc ggtcaacggg ctcggggcgc atgacatctc gatttcggac
    13561 gaaatgctgc tggcccttgc gcgggcctcg gacggcgatg gccgcaggat gctgaacctg
    13621 ctggaggcat gtttcggcca tgccgtcagg cgcggctccc gtgtggttga ccaggccgtg
    13681 atccaggcgg tcctgaccgg gccaggtctt cgttacgatc gcgcaagcga ggaacattac
    13741 aacgtaattt ctgccttcat caagtcgctg cgggggtcgg atccggacgc cgcgatgtat
    13801 tacatggtca ggatgcttga gggtggtgaa gacccgcgtt tcatctgccg tcgcatgata
    13861 atttttgcgt ccgaggacat agggaacgcc gatccgcgcg cgctggcggt cgcttgcgac
    13921 gccaaggacg ccctgctttt cctggggctg cccgaggccg agattccaat ggcccaggcg
    13981 tgcacgtacc tggcctgcgc tcccaagtcg aacagatcct acctggccct gcacgcggca
    14041 aaggacgcgg tgaaaaatac cgggtcgctg gaagtgccga tgcacataag gaacgcaccg
    14101 gtcaaggcaa tggcttcgca cggttacggc cagggatatg cctatccgca cgattacgat
    14161 gacgccttcg tgtcctgtga ctaccttccg gatgctcttg cgggcagccg cttctatcag
    14221 ccccgcgact gcggatatga aaagacgatg ggcgagcgtc ttgagtggtg gcggtccaaa
    14281 aggcgccccg atccgccccg cgatcggtag gggcgaatga aaattcgccc gcctcggaag
    14341 taaccgcacg ccccatgaat gacgcagtcg tgcctgtggc cggccatgaa gtgtccggtt
    14401 cgagaggcct caccggacac ttatggtccg gcccgatccg ccccgcgatc cgtaagggcg
    14461 aatgaaaatt cgcccgccgc ggtcgagcac gttgccttaa atcgcgccgt gcccttcgat
    14521 cctcacattt cgttgatcat cttcttgaag cggtcgccgc ggtcttcgaa actggtgaac
    14581 tggtcgaatg acgacgcccc cggcgacagc aggaccacgc tgtccgggtc ggcgatttcg
    14641 cgggcacggt tcaccgccga ccgcatgtcc tgaacgattt ccaacggaac ggccttcggg
    14701 aattcccgct tcatccgctc cgcgcatgca ccgaacagca ccacgtggcg gcatctttcc
    14761 ccgatcgcgg tcgccatgtc cttaagttcc aggttctttt cgtatccgcc tgccagcagc
    14821 acaagcggtc gcccggcaaa cgaccttatt ccgaccgccg ccgcgtgggg attggtcgcc
    14881 ttgctgtcgt tgtaccagtc aaccccgtca agatgacgga cgaattcgat ccggtgcggc
    14941 agtccggcgt acgtacgaag gccgttcacc agcgaaggga tgctgacccg ccgatcctga
    15001 agggccatac acgccgcgat tgccatcagc gcgttttccg tgttgtgcgg gccgggcaac
    15061 ttcaattcgt cccgcctgca cacttcaatc gcccttttcc tggatgcgat ccacagtccg
    15121 ccgtcctcca ggaacgcccc gtcctcgacg gagtgttcaa gggaaacgtc aacgaccgcc
    15181 tgcccgtttc cggatatcca gcggttgcct ggcgtgaacc acgtcttcag gacgggatcg
    15241 tcgatgttgc ggacgaaggc atcgccggcc atctgtgccg ccccgaccat cgcctttgcc
    15301 cggacgtaag cctcccatgt cccgtggtgg tcgatgtggt cgggcgccag gttggttacc
    15361 agcgccactt ccggcctgaa ctgcctgcac gttataagct ggaagcagct tacttcggca
    15421 acgaccacgt ccttttccgt aaggtcctgg acttccccgc acagcgcgtt gccaagattt
    15481 cccgcggccc gcgcgggaat cccgcatgca ttcagcaggt gtgccgtcaa ggtcgtcacc
    15541 gtgctcttgc cgtccgtccc ggtgaccgcg atggtgcggc cggggaagaa tcggaagaaa
    15601 agttcgacct cgccgattac ttcgtcggca actcgctggg caagcctgaa agccggagcg
    15661 tggggcgcga ttcccggtga aagtaccgcc acgctgccgg gcctgacaac ctcgcatccg
    15721 aagacggttt cgacccgccg atcaacggaa ccggccaccg attcccgcag atcccggtta
    15781 tcgcgcatat cggaaaggac gacctgggcg ccgtgcgagg caagcaggtt ggatgcggcg
    15841 atgccgcttt ttcccattcc gattacgcag aattttcttc cttcaacaag cattggggcc
    15901 cccttgagtc aatttcaccc ggcagatgaa acggcgccga gtcgctgcac cagttcgtcg
    15961 gcaagctgga aaaggtcatc gcccccgaag aatatgatcg tgtcgccatc gcgcagaacc
    16021 ttcaggattt ccggaatgat atcggtcatt tccgggatat acgccacatc tgaaccggcg
    16081 tcgcggatcc tgccgacaag ccagtccgta tctatccccg gaattggaac ctcaccggcg
    16141 gtgtacatgc gcgtgacgaa agtcaggtcg gcgcccttga aggcttcaga atactcgtcg
    16201 cccaggtaat ggatcatcgt gaaacggtaa ggcttgaaca cgcacaggat cctgcccgtc
    16261 gcgaacttgc gcgccgtatt gatcacgcag cggatgccgg tcgggtgcga tatgtagtcc
    16321 ttgaccacca gtccacggtc ggtccggacg cacgaaaatc ggttttcgag cccccggaac
    16381 gtgccgactg cagcccttac agtttcgagc gggacgccct cgtgaatcgc gacgcttatc
    16441 gccccggcca tgttcatcgc attgtattcg cccgggatgt gaaggatgaa ttcgcccatg
    16501 tccccatccg gtcccgacat ggaaaatacc gttgtcatcc cgacggttcg tatgtcgcgc
    16561 accacgaagt cgcacgaagg gccgcatccg aacgtgacgc ccggaaatcc gcttctgcgg
    16621 cgaacctcgg caatgttttc gtcgtcgccg tttgcatgaa gcctgcgcgg cgccatgggg
    16681 ccggcaaggt atcctgaaaa cagcgccagc aggccttcca ggtccttgta atagttaagg
    16741 tgatcggcct cgaggttgtt gatcaccatg acgtcagggc ggatgttggt gaaggaaccg
    16801 tccgattcgt ccagttcgca aacgattgtt tcgccgtccg tcgggcgtga attcgtctgg
    16861 aagttcagca gccttgcccc gattgcaaat gccggattga caccggcgca ttcgagcagc
    16921 cacgtaatca tggccgaaac cgagcctttt ccgttcgtcc cggtgacccc gatcgacttg
    16981 cggccgcgca tcaccgcatc aaggatcatc gaccggtgaa ccaccgggat tcccgccttg
    17041 cgcgccgcaa gcagttcctg gttcgtttcc ggtatcgctg tcgatacgac caccagatcc
    17101 gcgccgcgaa ggttttccgg gttgtgaccg atgaatacgt cggcaccggc gtttcgcatt
    17161 tcaatcgtca ggcacgattc gcgaacgtct gaacccgaga ccttgtggcc tgcgtgcagg
    17221 acctgtctgg ccacggcgct gaccccgacg ccgccgatgc caatgaagtg aaagtgtttg
    17281 aaatcgttaa gttcaatctg actcattgcg gtgctgtccg taaatactga aagatgaagg
    17341 ccgccgaatc tcccgcccgg gcggatcgct ctgccggtgg cgggttgtcc aacccagcct
    17401 gttatgcgtc ccgaaggata acccagtacg tgattattgt cttcaacgac acgggcccgt
    17461 gttatcggcc gcgttcgcgc agggattcgg caagcctggc cgcctccagg ccaagcgtcg
    17521 gtctgaccga ctggcctgcg atgatttcca gtgccctgac ggcatccggt ccgccgatgt
    17581 cggcaatggc gtatgtggcg gcccatgcga tagacccttc gttcgaaagg gtcaggcgtc
    17641 caagcgtaag gaccgtccgg ggaaccttca ggcgccccag cgtgccgatt gcacgaaggg
    17701 ccaggtcacc ggaagaagac agggccagtt cctcgacggc ggcgacgcag tcggtgttgt
    17761 tgcggacggc aagctcctcc agcgcccgca tcaggactgc aagtggcacg ggaccacgcc
    17821 ggcgtgaagc gatttcgcac agcttttcat cggcaacccc cgaaatcgcc tgaatttcgg
    17881 aaacctgacg gataagatcg aacagcgagg cacccaggga gaggaatccg tcttcgcagg
    17941 cgaagatcgt gtcggagttg gcgctttcaa acgtttccga ttccagcgtg cgggaaaaaa
    18001 ccttcagttg aagcatcgac gcgacttcga ttccgtctcc tgcggggcga cagccgagcg
    18061 cgattcgaag ggaatttccc ggcatcgcca tgtcgcgcgc cgcaagcagt gcgaagccgg
    18121 ccgcagcaag ggccggacag cagtcttcgg actccacttc cgtctggatt tcggccacct
    18181 tccatcgagc cggtggttcc gctggctgcg gtgtcgcgca cccgtgaagc gttgccgcgg
    18241 caagcgcggc tgcgacgagc acccgtccag tccaggccga aatggaaaac cgtcgcggca
    18301 tcatgggaac ccgtttcgtt tgcgacatga taaaataccg cgtttccaaa cggagggcat
    18361 gtccctttca tgaaatcgac aacgtcgtca accgaatcaa ggccgaacaa atcgaccatc
    18421 gagccaaggc tgctgaaggg cttcagggat tttctcccgg acgagcaggc ggcccggcag
    18481 acgatgcttt ccggtatcac cggggctttc gaactttgtg gttttgcacc actttccaca
    18541 cccgccatcg aatacgccga catccttctt ggaaagtatg gcgacgaagg cgaaaagctg
    18601 ctttacaagt tcgaggataa cggtggccgc caggtcgccc tgcgttatga cctgaccgtt
    18661 ccgctggcac gcgtggtcgc ccagtacagg gatattccga agccgttcag gcggtatcag
    18721 cttggcaccg tctggcgggc cgagaagccg gctcatggtc gcttccgcga gttcatgcag
    18781 tgtgacgccg acatcatcgg caccgcctcg tcccttgccg atgccgactg catcgcggcc
    18841 ggcatcatgg gattgaagtc ggtcgggctt gaacgctttg aagtcaggat cgggcaccgt
    18901 ggcgtcctga acgacgttct tggccagttc ggcgtgaccg tcccggacgc ccaggccgac
    18961 atcctgcgca acctggacaa actggacaag atcgggcccg gagccgtcgc ggaaacgatc
    19021 gcggcccagg ggttgatgga tgcggatcag gccggacgtc ttattgccac gatttcgggg
    19081 gccggcgtgc cgacggtcgt caccgaagcc ggactggccg gggccgaaca tttgcgtggc
    19141 gtccttcagg cccttgagtc gatgggtttt ggacctttca tccgggtcga tatgtcgatt
    19201 gcccgtggcc tggactatta cacgggcacc gtttatgaaa cgatgctgct ggatcttccg
    19261 tcggtaggtt cgatcatgag cggcggtcga tacgactgcc tgcttgagac ttacggcatg
    19321 cccgaggttc cggccgtcgg aat
//
LOCUS       DAOPPA010000005        18962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_3532_length_18962_cov_4.376051, whole genome shotgun sequence.
ACCESSION   DAOPPA010000005 DAOPPA010000000
VERSION     DAOPPA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18962
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_18962_cov_4.376051"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..579
                     /locus_tag="PKH54_00380"
     CDS             283..579
                     /locus_tag="PKH54_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOC98370.1"
                     /translation="MLNEGARGTVIEISVRPRAGKCRIVEVGPDRVRIEVTAAPEGGK
                     ATEQALRTLADALGVMASGLKLLTGKTARHKTILVCGATAADCESRLKQAVAGH"
     gene            601..3195
                     /gene="lnt"
                     /locus_tag="PKH54_00385"
     CDS             601..3195
                     /gene="lnt"
                     /locus_tag="PKH54_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOC98371.1"
                     /translation="MARKNPSAKKTTSGIGPIVPIWLAILLAIFGGCLIFLSFPTFDI
                     FPLSFFGVAMMLLAIRGRGAAAGFWLGLLTGFVTNLGGFYWISYMLREFAHMTVWLSW
                     VITCMMVAVQGLVYAIPAAISSGFANRFPKLPWMLFFPVIFTAVEYLFPLIFPWYLAN
                     GQQKFTALIQMADITGAAGITFLIILVNCAVADIAARRISGTGFPAVSVAVASVALVA
                     ALAYGMFRIDQIDQRMEAAPKLKVGMVEADIGIWEKEARNQDGSPLDGAGQMAMLYSN
                     LLKHQYLSQTLEREHKPDLIVWPESSYFPLYQVFSRRNPASLLAVTAGGSLVGVTDSS
                     ATVDAESSKVFRDSGLTAISAQNEENILAVGPRGAIYKFDGVKYLREESGTDRNLNAV
                     AWSFDDGSAMAVGDNGVVLLRELDQDSKASWKVVDTGRNFTLRGVVKTPKYGWVACGD
                     NGSLISIDDGRVRSLVPDGLPDLTAVTWSDKLGVVAVGHQGAIVTMSGPNEVKMEQPS
                     KKTLRAVWADSLIWAVGDDGLVVLCDSTCHTVKSGVRRTLNAIGYMGRGSLVAGGLDG
                     ILLELSQDKGPRRIEGASGRITGITDLPFVEGYPFPTDVKKIYVSRTPLPQEGSAGDL
                     LPAYESDRKRPPAERNSVMRGFSTPMIFGAATQERFPAPGKAPVRHNTAIMMDGSGQV
                     LGTYFKNRLLIFGEYIPLEGLFPIFRKWLPEAGDWTPGDGPALFALGDAKIGISICYE
                     GLLAGFHRKLAALEPNVYVNITNDAWFGRTHEPWLHLQLAELRAVEARTPMVRSTNTG
                     ISAFVDAVGRRTAHTAIEGAEVLVGEVALTSEKTIYVRYGDVFAWACCGIAAIMILAL
                     AAGRRRRA"
     gene            complement(3152..3769)
                     /locus_tag="PKH54_00390"
     CDS             complement(3152..3769)
                     /locus_tag="PKH54_00390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC98372.1"
                     /translation="MGESGNRPGRWRLLVAGLVFYAVAGILSAAAARAFPRTFGFDAF
                     PADARSWAVSILAGAAVAAILIIASWLLVFFSASGRRMASVLASATKELSPAGALSLA
                     VMAGISEELVFRGALWSLVESMFGRWWAFGVTSVLFAAVHGLFRRGLVLWGVFALAGG
                     LAAGGLYMMTGSLVAPVAMHVIVDAVNLPALVMRGGAGRRQAQES"
     gene            complement(3762..4778)
                     /locus_tag="PKH54_00395"
     CDS             complement(3762..4778)
                     /locus_tag="PKH54_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOC98373.1"
                     /translation="MKGFILAAGLGTRLLPYSARIAKPAFPVLGMPLVEWVVAGLRNA
                     GVTELVVNLHHIPESVVGVLGDGSRLGVGITYSHEERVLGTGGALVRALDLIGDSDGV
                     LVHNGDIFSDWDIGRLVGASARTVLGVLDGSRLDPSERRVELAPDGSVSALRGLPAPG
                     GGRKVVYGGVSVVGPQILEILRDTVARRGDDSFSPCLVGDGLIPALRLGIRPASVEFD
                     DTWYCDIGTIGSYLDLCSRAIPAAPMLLSRRGFPVPPEIGPSVFVSGRARIDSDVVFH
                     GPAFVSDGAWVGTGATIGPNVTCSGRIMPGSDVGNAVVMAGAVVSGKFSGIALPDGPV
                     KNNG"
     gene            complement(4781..5821)
                     /locus_tag="PKH54_00400"
     CDS             complement(4781..5821)
                     /locus_tag="PKH54_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC98374.1"
                     /translation="MMSGSGEDAARAFRDFSGGTVPVGWEVTRLPGDASNRSYFRLSG
                     PGRFMVMKLAAEPFSSEEGPSGGASRRLPFCDVADYLSRGNVAVPEILVTDLDAGFLV
                     LEDLGDVTLERALAAGGNRRDLYRWAIDVLVDMQYWAFLNPDPSNVAFKRSFDADLLM
                     WEFDHFREWGLVEMCGREPDAADSALLKAFFQDVTSRLTAMRQGFVHRDYQSRNLMIK
                     DGRMRVIDFQDALVGPFIYDLVALLRDSYVKFSPSEVDDFLSYYHEVGRPLMPWIPDI
                     KEIRRLFHLQALQRKLKDAGRFVFIDRVKGNPKFLPNIPQSLAYVAEAFDHLPEYAQA
                     RAVLKRYLPEHF"
     gene            complement(5951..9304)
                     /locus_tag="PKH54_00405"
     CDS             complement(5951..9304)
                     /locus_tag="PKH54_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98375.1"
                     /translation="MKSLLKYVAAAVLFAGLYVPGALALDAVPHGINIQGVLRNVGGE
                     VVTGTYSVKFTIYDAIGGGAVLCQNITSISVTGGVFNTSVTCPATAFVNRTAAYLGIQ
                     VGSDAELARIPLTSVGFAIQAEHAEQADELLGAATDVNCVGCIGDNEVSFPYAGSTTR
                     GGPATDVNCNGCVSGGAPGDIAAGSITTNNIGAGQVQNGNIADGAISNSKLGANSVKT
                     GNIEDGQVTTNDLATGAVTTVKIGTGAVGDTQLGVNYALGTSKGGPAADLNCSGPCVS
                     DLEAGFNYALGTTKGGPSANLDCLAIPGGCVEAGEVSFQYAAGATKGGAAVDLACTDC
                     VGSGEVQFNYAGSTSEGGPASDVACTNCIASTEVNFNYANSTSRGGDATGLACGSACV
                     STNEVDFNWAASATKGGPANDLLCVGCVDSGDISDGTIVNADITGPITGSKITTATTT
                     DLGVVKIGSGIAISSGSITPDWTQVAQKDHNHGDQYFEQGIDHLTLGDGKYIDFLDGS
                     DNPGSYPARLQEVSTGGDGNAQIRLSLGQAAAGSGSYFEIEGTGAYTGARHRLFANGN
                     AYHAGSVQMDGALSCTGCVDTTDIADGTVTTNDIYDGTITGADLNPALQLTTTGKVTA
                     GEVWAQKFVDVTGSPNWYADPQATSVMNTINASNFQLRTNNYYGLKTNGGGTMSLYTS
                     SIDAANGETLYIAKNACGLIDVYGTTCGSNIMTIHGTLKAKSLVDEDSGAYVVDPASH
                     SKIATLETNTVQLPATGTTTNYINGTTYAFDTQWNDGATGYYLDPSSASYFNVHYGNS
                     FRTTGSWQGDPPNGYGQFASSGTNLYISPAPNGSAFVLMRFAGNDRMVFSLNGNVSAG
                     IPSFRTNGGYLVINSGSSDKRLLLNWDQSGGTTEVVGTFKAPSMVDSVSPTCVMDAGN
                     TSNAYEIYSQYISTYYLRVHQGLRIDGTNGLFVVGNGDWGTGIRMYGNTSIIPHTDGQ
                     GAVGMSGKRFAFMYSINSVWTSNRELKKDIRYLNKRDMDSIGKALQQIKPATFLYNHE
                     VAAAEANESTDKMFIRNVPHIGLILDEMPEIITNGGDGWYANDSVGFLLVASKYLDDK
                     INRQAETIRDLEDRIAIMEQAIADAGLFN"
     gene            9697..10593
                     /locus_tag="PKH54_00410"
     CDS             9697..10593
                     /locus_tag="PKH54_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98376.1"
                     /translation="MRRFLQVAAGNTGTLAMIAALAALSIGCGGDRRPEKTPPAQAGE
                     GPAEKAPAPDDDAKPDSRQNKAGPRVSAKADQKADKPTATDSQAVPGPADMAAKPEIR
                     VEQPQAARNPGQVQAAEPVSATTAPAAQTAAAGQAPAPGASNPADSPQPVPKPDPSRP
                     PLPDPRLLLTMTDVAAIAPARARFQRAPLAGAPRTEDSDAILYRPEKGNGFGFSLQIF
                     RSRNALETRKRFDSLLASYPSAQEIASVSGKTFFSYWGDTIHIGFVQPSRNLTAIVSC
                     GRDFCDSDKLMELSTKIAERLR"
     gene            complement(10590..12743)
                     /locus_tag="PKH54_00415"
     CDS             complement(10590..12743)
                     /locus_tag="PKH54_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOC98377.1"
                     /translation="MTTLSGEVSKLRYFDEERGFSVFDVIVSLPEDISGTTVTAVGPL
                     PGVTGGMEVRLSGDFRNHPRFGVQFRFGSGMQVPPSSGRGIMAFLSSGAVEGVGKILA
                     RRLFDHFGEGLRLALDTGPEKLTSCPGIGSGRAAAIWKAWKKGIEAREAVIVLCDMGL
                     LPAQASRIAGLWGASAPSIVASDPWSLQRHLRGYGFKSADRVASRIGTDPLAPARLKA
                     AVAHALAGAQDSGHMFLREDVLLESASGLVDLTPRQVVPGLEALVSAGEVVREDRAGE
                     GAVVYASGAHQAELGAAVGLAALCSGAPAPIPVVACPGESDLAPSQMEALRTLCSFPV
                     SILTGGPGTGKTTVVGRLMTICRKSGIRVSLAAPTGRAAMRMREASGVEALTLHRLLE
                     YNPATDRFVRGPDNPLEADWVVVDESSMIDMFMFHALVAAIRPGTRLTLVGDADQLPP
                     VGPGDPFREAIRSGSVPVARLTQVFRQGDGSSIVSAAHEIMAGRVPSSDRSSGSDPGD
                     FHVVFRESAEEAADLAVALVTERIPARFGLDPRQDIQVLAPMKKGSCGTDELNERIRR
                     RLNPGVDDELAAQTPFLPGDRVMQTRNNYDLDVFNGDIGTVAGPSGDNGLLVRFDRRI
                     VRIPAADMDDLCQAFAVTVHKSQGSEFPAVVVCMHNQHFVMLRRNLLYTAVTRGRRVV
                     VLVGSRKAIGMAAGNTMEEGRNTLLASRLRECRQP"
     gene            12905..15205
                     /locus_tag="PKH54_00420"
     CDS             12905..15205
                     /locus_tag="PKH54_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98378.1"
                     /translation="MKNLVSTLLVVLSALAPMALDAEPLPLVYEAVTLATTDGKPRIA
                     VFPRSVPLQMAQGRTADTPSVQRIFETMKARFPAAYGTTTLSISGGREPVATINLDPS
                     RKETWGLVTAEIFYTLKSFGFENIVAPLYLQDPLNPSLIDAVVMVPVQPWFNAAGEDF
                     PSTIAVSLDGSRMISTSLFRQLLTTGNPDVARIVQAALSAPLEAQRLAAVRALAAQKP
                     VSADLVARISSLLTDPSPAIRGAILELAEARKIQVALEDLRKVADADQDPALRLAAAK
                     LLSAAGVRDYDHILEVRNLRSGNPTDIAQSLRRLKTSGKSDIAPAVAETLESPDETVR
                     NLAVEVLLSMKAATVVAAALQNPKVQPAQKTVLASGLLSMCPGDDSAIAWMAGQPAMA
                     GRALAALCGTPAQPPAYCRRSSGVLPVIPLFLKRPETEIRLKAIECLSTRNEQEVLDL
                     LIPMTTTAGTEQAATAAASAVLSRQPQAVLLGIISGRGVPAARATRQFRLLALNGLAS
                     QAQSTALEPSALAILKGLMKDPDIEIRRAATGVMARSRDAAVAALVLDGINDQDEGIR
                     TAAVIAAGRIPGPASDAAIRKSLEDLSVQVRAAGLEILASRPLEGTRPKLQMLAQNQR
                     PEIRRPAVAAFLKTLGPGDDAVRTQFVSTLLYDNSEEIRIIAVRAMEEMTDTQSARAV
                     GSLVIDRSVPVRVEVARVLGQMRNDVSLDYLKKMARDQDFQVRKTVIEAVGRFPVASS
                     TPAFREMLGTESDGTLKALIEQKLAR"
     gene            complement(15257..16702)
                     /locus_tag="PKH54_00425"
     CDS             complement(15257..16702)
                     /locus_tag="PKH54_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98379.1"
                     /translation="MTCRAAFVGILVCLAGLITAVPVTGSEVVRPASKKVILIPGRDF
                     RIDPDKLFFDPSEPPPRLQEMPQLIREASAVLAAEPEIELVDLSSGQPVSAPQGTASR
                     HLVVMGMLHLGIERYRDIRLPEAISSLEDGISAALASDSDVLVPAVVSDLYLYLGLSR
                     LETSEPDLAHIALKQMFGHMPERRFRQGWFPAREERALRAAALDFVKSPARENPFEST
                     ERALGFLRERGADALVYVFLESDPEGGDRLETRVFEVSRKDAGSVEVMRDSRPYRGVE
                     SVSMAISSWLACADLPSRTDTLKRLPRVFLDTGFSYSMFAQDNSTRTGFHNIGLSIGT
                     AYQIQPGLDTFLRLSFYQAREDSYGDLLDDFFSFRASIGVGYSAVFNWGRVFTHFGFE
                     MDYLSGFKSSTEPRCKLWPDDPDLCPSSQVTEPSFLFGAVGLLGVNVFVSRSVFVMVQ
                     AGISGYFVSNSALVDLNFPFVVEFGFGYAFF"
     gene            complement(16699..17274)
                     /locus_tag="PKH54_00430"
     CDS             complement(16699..17274)
                     /locus_tag="PKH54_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98380.1"
                     /translation="MSVRLSAVCPALSAALFLAGLVSCDSSRMISGIYQSDLPVVIDG
                     VPGLDTPRYFEMMLAQFGPDITGIVRVYDDADFLVPSSEVCPCRFVENGKVESSSMAL
                     SFRSHGDCPGSSGTPLIIGRFTFKEPVVVDDEAGELLSGTLELQDAGEPGQVDVTFRR
                     ARTWRQVGEADLKCGDPLLYADDPPETGDAG"
     gene            complement(17301..18122)
                     /locus_tag="PKH54_00435"
     CDS             complement(17301..18122)
                     /locus_tag="PKH54_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98381.1"
                     /translation="MFGNKELQDVRRELDLALQRAEACQNEIGQMRARLASVDADLKN
                     AARDASALQSEVAAAKAEALRLADCRERADKAAAFYEEKYETLKREIDMHAAAAQEAR
                     IAAGAASEESESLKARLAASMAENERLKARLARLEESGAAKAAPRPVEEVPDGGDARL
                     LKAELETMRRRVAEAEEMRRVALRKAEHNRRAWLVTQMQLDLAEDRLCLLTTGKPRPV
                     LESRLSVEVGEGEIIEAEDVEDEPGAAVVSEPTPTPSPSDHGDEPGDAGGSGLPG"
     gene            complement(18183..18596)
                     /locus_tag="PKH54_00440"
     CDS             complement(18183..18596)
                     /locus_tag="PKH54_00440"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOC98382.1"
                     /translation="MFRRFLRSKIHRATVTDADVNYVGSITIDSDLMDAARLAPVEEV
                     EVWNVTTGSRFSTYCIPGPRGSGVVQINGAAAHLAHRGDKVIIAAWATLDSSELGAHV
                     VPVVVPGEGNLSFRMMEYRADLVAGNFEIVDLDPA"
BASE COUNT         3934 a         5893 c         5683 g         3452 t
ORIGIN      
        1 agacctgatc gccgaaaagc cttggaaagg ccaggctggt gaacgtcgcg gtaagcagga
       61 accacagctt ggcgccggtt atcaggaaga aaccgcgccc ggcctgtctg atcgaaccat
      121 ccgaatccat cgggacgccc ccggatcgac aggcatcttc ttaccacctg gcagtcaaaa
      181 ccgcaaagat ggcgttctcg gtcatccaac ttcgaccttc ctttccgact gccgcgcgcc
      241 ggatgctata atgccgcacg gaaactggca ggctcgggca ccatgctgaa cgaaggcgcg
      301 cgcggaaccg tgatcgagat aagcgtccgt cccagagccg gaaagtgccg catcgtcgaa
      361 gtcggtccgg acagggttcg aatcgaggtc acggcggctc cggagggagg aaaggcgacc
      421 gaacaggccc tgcgcaccct tgccgatgcc ctcggagtca tggcttcggg actgaaactc
      481 ctgacaggaa agaccgccag gcacaaaacg atcctggttt gcggggccac cgcggccgac
      541 tgcgaatcaa gactgaaaca ggccgttgca ggacactgag ggccgacatc aggaattgag
      601 atggcacgaa agaacccttc cgccaaaaag acgacttccg gtatcgggcc gattgtgccg
      661 atctggctgg caatcctgct ggcaatcttc ggcgggtgcc tgatcttctt gagcttcccg
      721 acgttcgaca tcttcccgct gtcgttcttc ggcgtcgcca tgatgctcct ggcaatccgt
      781 gggcgtgggg ccgccgccgg cttctggctt ggactcctga caggttttgt cacgaacctg
      841 ggcgggtttt actggatatc ttacatgctg cgggaattcg cccacatgac cgtctggctg
      901 tcgtgggtaa tcacctgcat gatggtggcg gtccagggcc ttgtctatgc gattcctgcc
      961 gcaatatcct caggctttgc caacaggttc ccgaagctgc cctggatgct gttcttcccc
     1021 gtgatcttca cggccgtcga atacctgttt ccactgatat tcccatggta tctggcaaac
     1081 gggcagcaga agttcacggc gctgattcag atggccgaca tcaccggggc cgccggcatc
     1141 acattcctga taatccttgt caactgcgcg gtggcggaca tcgccgcacg tcggatttcg
     1201 gggactggat ttcccgcagt cagcgtcgca gttgcttccg ttgctctggt tgccgccctg
     1261 gcatacggga tgttcaggat cgaccagatt gaccaaagga tggaagcggc cccaaaactg
     1321 aaggttggaa tggtcgaggc cgacatcggg atctgggaaa aggaagcccg gaaccaggac
     1381 ggatctccgc tggacggggc cggccagatg gcgatgctgt actcaaacct gttgaaacac
     1441 cagtatcttt cgcagaccct ggaaagggaa cacaaaccgg atctcatcgt ctggccggaa
     1501 tcttcctact tcccgctcta ccaggtcttt tccaggcgca accctgccag cctgctggcc
     1561 gtgacggccg gaggaagcct tgtcggcgtg acggacagtt ccgccactgt tgatgcggag
     1621 tcgtcaaagg tattccggga ctccggcctt acggcgattt ccgcgcagaa tgaggaaaac
     1681 atcctggctg tcgggccgcg gggcgcgatc tacaagttcg acggcgtgaa atatttaagg
     1741 gaagaatccg gcacggatcg caacctgaac gctgtagcgt ggtccttcga tgacggctcc
     1801 gcgatggcgg tcggagataa cggggtagtg ctgctgaggg agctggatca ggactcaaag
     1861 gcttcatgga aggtggtcga taccggacga aacttcacgc tgcgcggtgt cgtcaagaca
     1921 ccgaagtatg gatgggtggc ttgcggggac aatggcagcc tgatctcgat tgacgacgga
     1981 cgtgtgcgca gccttgtccc ggatggactt cctgacctta ccgccgtcac ctggtccgac
     2041 aagcttggcg tggtggccgt ggggcaccag ggtgcaatcg tcacgatgag cggcccgaac
     2101 gaagtgaaaa tggagcagcc ctcaaagaag acgctgcgcg ccgtatgggc cgattccctc
     2161 atatgggcgg tcggggacga cggcctggtc gtgctgtgcg actcgacctg ccacacggtc
     2221 aaatctggcg tcaggcgcac gctgaacgcc atcggctaca tgggcagggg ttccctggtc
     2281 gccggtggcc ttgacggcat cctgctggaa ctgtcccagg acaaggggcc tcgaaggatt
     2341 gaaggcgcct cgggacgaat caccggcatc accgacctgc ctttcgtcga aggttatcca
     2401 ttcccgacgg acgtgaaaaa gatctatgtt tcacggactc cgctgccgca ggaaggttcg
     2461 gccggggatc ttcttccggc ctacgagtcc gacaggaaga ggccgccggc cgaacgcaat
     2521 tcggtaatgc gcgggttttc aactccgatg atattcgggg ccgcgaccca ggagaggttc
     2581 cccgcccccg gcaaggcgcc cgttcgacac aatacggcga taatgatgga cggctcgggg
     2641 caggtgcttg gcacgtactt caaaaaccgc ctgctcatct tcggcgaata cattccgctg
     2701 gaaggacttt tcccgatttt caggaaatgg ctgcccgagg cgggagactg gacgcctggc
     2761 gacggaccgg ccctgttcgc gctgggcgac gcaaagatcg gcatatccat ctgctatgaa
     2821 ggtcttctgg ccggtttcca caggaagctg gcggcgctgg aacccaacgt ttatgtgaac
     2881 ataacgaacg acgcctggtt cgggcgtacc catgaaccat ggctgcacct gcaactggcc
     2941 gaactcaggg cggttgaggc caggacgccc atggtgcgtt cgacaaacac cgggattagc
     3001 gcctttgtcg atgccgtcgg cagacggacg gcacacaccg cgattgaggg tgccgaagtc
     3061 ctggtcggtg aagtggcctt gaccagcgaa aaaacaatat acgtgcgata cggcgatgtt
     3121 ttcgcctggg cgtgctgcgg catcgccgca atcatgattc ttgcgcttgc cgccggccgg
     3181 cgccgccgcg catgacaagc gccggcagat tgaccgcatc aacgattacg tgcatcgcga
     3241 caggcgcgac aagcgatccg gtcatcatgt aaaggccccc ggccgccagc ccgccagcca
     3301 gggcgaacac cccccacaga accagcccgc gcctgaacag gccgtgaacg gcggcaaaca
     3361 ggacggaagt cacgccaaaa gcccaccacc tgccaaacat cgattccacg agggaccaga
     3421 gtgcgccacg gaagacaagc tcctcggaaa tgcctgccat cacggccagg gaaagcgcgc
     3481 cggcgggcga aagttccttt gtggcggaag caaggacgga tgccattcgc cggcccgagg
     3541 ctgaaaaaaa taccagaagc catgaagcaa tgatcaggat tgcggcaacc gccgcgcctg
     3601 caagaatgga cacggcccag gatcgggcat cggcgggaaa cgcatcaaag ccgaaagtcc
     3661 ggggaaaagc ccgggcggcg gcggccgata gaatgcccgc gacggcataa aagaccagcc
     3721 ccgcgacgag cagccgccaa cgacccggcc tgttcccgga ttcacccatt gttcttaacc
     3781 ggcccatcgg gaagggcaat accagagaac ttgccggata ccaccgcacc tgccatcaca
     3841 acggcattgc cgacatccga acccggcatt atccggccgc tgcaagtgac gttcgggccg
     3901 atcgtcgcgc cggttccaac ccatgcgcca tcggaaacga aggccggacc gtgaaacaca
     3961 acgtccgaat cgatccgggc ccgaccggat acgaataccg acgggccaat ttcgggaggc
     4021 acggggaatc cccttcggga aagcagcatc ggggccgccg gtattgccct tgaacacaga
     4081 tcgagatagc tgccgattgt cccgatgtcg caataccacg tgtcgtcgaa ttcaacggag
     4141 gcaggcctta ttccaagcct caaggccggg atcaatccgt caccgaccag gcacggactg
     4201 aaagagtcgt cgccgcgcct ggcaacggtg tcacggagaa tctccagaat ctgcgggcca
     4261 acgactgaaa cgccgccata aacgaccttc ctgccgccgc cgggcgccgg cagtccacga
     4321 agggccgaca cggaaccgtc gggcgccagt tcaacccttc gttccgacgg gtcaaggcgc
     4381 gagccgtcca gcaccccaag caccgtccgg gccgatgccc cgacaagccg cccgatgtcc
     4441 cagtcgctga agatgtcgcc gttgtggaca agaacgccat ccgaatcgcc aatcaagtca
     4501 agggccctga ccagcgcacc tccggttccg agaacccttt cctcgtgcga ataggtgatc
     4561 ccgacaccca gccgcgaacc gtccccgagc acgccgacca cggattcggg aatatggtga
     4621 aggttgacaa ccagctcggt cacgccggca tttcgcaacc cggcaaccac ccactcgaca
     4681 agcggcattc caagcactgg aaatgccggc ttcgcgattc gggcggaata ggggagcagc
     4741 cttgtgccga gcccggccgc cagtatgaac cccttcatct tcagaaatgc tccggaaggt
     4801 accttttcag aaccgccctg gcctgagcgt actcgggaag atgatcgaac gcctcggcca
     4861 cgtacgcaag cgactgcggt atgtttggaa ggaacttggg atttcccttg acccggtcta
     4921 tgaacacgaa ccgccccgca tccttcaact tgcgctgcaa ggcctgcagg tggaaaaggc
     4981 gccggatttc tttaatatcg ggaatccatg gcatcaacgg cctgccgacc tcgtgataat
     5041 acgaaagaaa atcgtcaact tccgaaggac tgaatttcac gtaggaatca cggagcaggg
     5101 ccaccaggtc ataaataaag gggccgacaa gcgcgtcctg aaaatcgatg accctcatcc
     5161 ggccatcctt gatcatcaga tttcgactct ggtaatcccg atgcacgaac ccctggcgca
     5221 tcgccgtcaa cctggatgtt acatcctgga agaacgcctt caataacgcc gagtcggccg
     5281 catccggctc tcgcccgcac atctcgacca atccccactc gcggaagtga tcgaattccc
     5341 acatcagcag atcggcatcg aacgaccgct tgaaggcaac attcgaaggg tcgggattga
     5401 gaaaggccca gtactgcatg tcaacaagca cgtcgatggc ccatcggtac aggtcacgac
     5461 ggtttccgcc cgccgcaaga gcccgttcga gcgtgacgtc gccaagatcc tcgagcacca
     5521 ggaaaccggc atcaaggtct gtaaccagga tttccggaac cgcgacattg ccccgcgaaa
     5581 gataatcggc cacgtcacag aaaggaagtc gccttgaagc cccccccgac ggcccttcct
     5641 cgcttgaaaa gggctcggcg gcaagcttca tgaccatgaa ccggccgggg ccggaaagcc
     5701 tgaaatatga cctgtttgaa gcgtcgccgg gaagcctcgt cacttcccag ccgacgggaa
     5761 cggttccgcc tgaaaaatcc cgaaatgcac gcgccgcatc ttcaccggat cccgacatca
     5821 tccaaacccc gaaagattca cgcagagcat aacgcattcg ggccgcgacc ggaaaacgca
     5881 tggcgcccgg ggacacgaag ctgtgcctgc aatgaagtgt tacgtcaggc tgtcgggggt
     5941 aatatcagcg tcagttaaaa aggccggcgt cggcgatcgc ctgttccatg atggcgatgc
     6001 ggtcttcaag atcacggatg gtttccgcct gacgattgat cttgtcgtca aggtacttgc
     6061 tggcaaccag caggaaaccg accgagtcgt tggcgtacca tccatcaccg ccgttggtga
     6121 tgatttccgg catttcgtca agaatcagac cgatgtgcgg gacgttacgg atgaacatct
     6181 tatcggtgct ctcgttggcc tcggccgcgg cgacttcgtg attgtagagg aaggttgccg
     6241 gcttaatctg ctgcagcgcc ttgccgatgg aatccatgtc gcgcttgttc agataacgga
     6301 tgtccttctt cagctcacga ttcgacgtcc agacgctgtt gatcgagtac atgaacgcga
     6361 agcgctttcc ggacatgccg accgcgccct ggccgtcggt atgcggaatg atgctcgtat
     6421 ttccgtacat gcggataccg gtgccccagt cgccgttacc gacgacgaac aggccgttcg
     6481 ttccatcgat gcgcagaccc tgatggacac gcaggtaata cgtcgagatg tactgggagt
     6541 agatctcgta ggcgttggaa gtgttaccgg cgtccatgac gcaggtcggg ctgacgctgt
     6601 caaccatcga cggcgccttg aaggtgccga cgacttcggt cgtgccgccg ctctggtccc
     6661 agttcagcag caggcgcttg tcggaggaac cggagttgat caccaggtat ccgccgttgg
     6721 tccggaagga cgggatgccg gccgaaacgt tgccgttaag gctgaagacc atcctgtcgt
     6781 ttccggcaaa gcgcatcaga acgaaagccg acccgttcgg tgccgggctg atgtaaaggt
     6841 tcgtgccgga ggatgcgaac tggccgtaac cgttgggcgg gtcaccctgc caggaaccgg
     6901 tggttctgaa gctgttgccg taatgaacat tgaagtaaga cgcgctgctg ggatccaggt
     6961 agtatcccgt cgcaccgtcg ttccactggg tatcgaaagc gtacgttgtt ccgttgatgt
     7021 agttggtcgt cgtgccggtc gcgggaagct gaacggtgtt cgtttccagg gtcgcgatct
     7081 tgctgtggga agcgggatcg accacatacg cgccggaatc ttcatcaacc agggactttg
     7141 ccttcaacgt tccgtggatc gtcatgatgt tcgatccgca ggtcgttcca tagacatcga
     7201 taagaccgca ggcgttcttg gcgatgtaaa gggtctcgcc gttggccgcg tcgatgctgc
     7261 tggtgtaaag cgacatcgtc ccgccgccgt tcgtcttcaa accatagtag ttattggttc
     7321 gaagctggaa gttggacgcg ttgatagtgt tcatcacaga ggtcgcctgg ggatccgcgt
     7381 accagttcgg cgaaccggtg acatcgacga acttctgggc ccagacttca cccgcggtca
     7441 ccttgccggt ggtcgtgagc tggagggccg ggttcaggtc agcgccggtg atggtgccgt
     7501 cataaatgtc gttggtcgtg accgttccgt cggcaatatc cgtggtgtca acgcagccgg
     7561 tgcaggaaag cgcgccgtcc atctggacgg agccggcgtg atacgcgttg ccgttcgcga
     7621 acaggcggtg cctggcgccg gtgtaggcgc cggtgccttc gatttcgaag tacgaaccgg
     7681 aaccggccgc ggcctggccg agggaaagcc tgatctgggc attgccgtcg ccgccggtcg
     7741 aaacttcctg caggcgggcc ggatacgaac cgggattgtc gctgccgtcg aggaagtcga
     7801 tgtacttgcc gtcgccgagg gtcaggtgat caatgccctg ctcgaagtac tgatctccgt
     7861 ggttgtgatc cttctgggca acctgggtcc agtcgggcgt gatcgaaccg gatgaaatcg
     7921 cgatgccgga gccgattttc acgacgccga ggtcggtcgt ggtcgcggtg gtgatcttcg
     7981 agcccgtgat cgggccggtg atgtcggcat tgacaatcgt gccgtcgctg atgtcgccgc
     8041 tgtcaacgca gccgacgcac agcaggtcgt tggccgggcc acccttggtg gcggaagcgg
     8101 cccagttgaa atcgacttcg ttggtgctga cgcaggcgct gccgcaggca aggccggtcg
     8161 cgtcgccacc ccgggaggtc gaattcgcgt aattgaagtt gacttcggtt gatgcgatgc
     8221 agttcgtgca ggccacgtcc gatgccggac cgccctcgct ggtcgagcca gcgtagttga
     8281 actgcacttc accggagccg acgcagtcgg tgcaggccag atcgacggca gcgccgccct
     8341 tcgtggcgcc ggccgcgtac tggaacgata cttcgccggc ctcgacgcag ccgccgggaa
     8401 tcgcaaggca gtccaggttt gccgacgggc cgcccttcgt ggtgccaagc gcgtaattga
     8461 aaccggcttc aaggtcggaa acgcacggcc ccgagcagtt cagatccgcg gcaggcccgc
     8521 ccttcgacgt accgagcgcg tagttcacgc cgagctgggt gtcgccgacc gcgccggtgc
     8581 cgatctttac cgttgtcacg gcgccggtgg ccaggtcgtt cgtcgttacc tgtccgtcct
     8641 cgatgttacc ggtcttgacc gaattcgcgc ccagcttcga attgctgatc gcgccatcgg
     8701 cgatgttgcc gttctgaacc tggccggcgc cgatgttatt ggtcgtgatg gatccggcgg
     8761 cgatgtcgcc gggtgccccg cccgaaacgc agccgttgca gttcacgtcg gtggcgggac
     8821 cgccacgcgt cgtactgccc gcgtacggga acgatacttc attgtcgccg atgcagccga
     8881 cgcagttcac gtcggtcgcg gcgccaagaa gttcgtccgc ctgttccgcg tgttcggcct
     8941 ggatggcgaa cccgacggat gtcagcggga tgcgggccag ttcggcgtca ctgccgacct
     9001 ggatgcccag ataggcggcc gtcctgttga cgaatgccgt tgccgggcag gtgaccgacg
     9061 tgttgaacac gccgccggtc acggagatgc tggtgatgtt ctgacacaga acagcaccgc
     9121 ctccgatcgc atcgtaaatc gtgaacttga ccgaatacgt tccggttaca acttcaccgc
     9181 ccacgtttct caagacaccc tggatattga tgccgtgagg aacggcatca agggccagcg
     9241 cgcctgggac gtacagtccg gcaaaaagca cggctgcagc cacgtacttg agaagcgact
     9301 tcatctgggt cacctccagt cctgaaactt cattgtcatc aacgtaccac cgtcggcatg
     9361 ccagccggca gcaacccccg ggagaccatt tttcgccaat tatcgccacg tggtatgcat
     9421 tattttcata acaacgcacg ccacatccac gtcctgaaaa accaaaaaac cgagatttca
     9481 aggctttaca gggcgcggga cgactcaagc gaaagtcgac cccacaccga gttcatattt
     9541 tagcactctt cgtgccatgc tgcaccagat cttttttatt tcgcacggat ttgccaaaac
     9601 gtttctacag gtcggcccgt ccaaccggat ttgacatcat ttgacataag ggggccgtca
     9661 aatctatcct cgcgacaggc tttcggggag gtgcccatgc gccgatttct tcaggttgcg
     9721 gcgggaaaca cggggacgtt ggccatgatt gccgcgttgg cggccctgag tatagggtgc
     9781 ggcggggatc gccggccgga aaagacgcca ccggcccagg ccggcgaagg ccctgcggaa
     9841 aaggctcccg ccccggacga cgacgcgaag cccgacagca ggcagaacaa ggcaggcccc
     9901 agggtatcgg caaaagcgga tcagaaggcg gataagccga cggccacgga ttctcaggcg
     9961 gttcccggcc cggcagacat ggcggcgaag ccggaaatcc gcgtcgagca gccgcaagcg
    10021 gccaggaatc ccggacaggt tcaggccgcg gaaccagttt ccgcgacaac cgccccggcc
    10081 gcacagactg ccgccgctgg tcaggcgccc gcgccgggag cctcgaaccc ggccgattcc
    10141 ccccagcccg tgccgaagcc tgatccctcc aggccgccac tgccagatcc aagacttctg
    10201 ctgacgatga ccgacgtggc ggcaatcgcc cctgccaggg ccagattcca gcgcgccccg
    10261 cttgccggtg ctccgcggac ggaagactcc gacgcgatcc tttaccggcc cgaaaaggga
    10321 aacggcttcg gattctcgct tcagatattc cgcagccgaa acgcgctgga aaccaggaag
    10381 cgcttcgatt cgctcctggc gtcatatcct tcggcccagg aaatcgcttc cgtatccggg
    10441 aagactttct tttcctactg gggcgacacg attcacatcg ggttcgtcca gccctcgcgc
    10501 aacctgacag ccatcgtatc ctgcggcagg gatttctgtg acagcgacaa gttgatggaa
    10561 ctatcgacga aaatcgccga acgcctgcgc tagggctgcc tgcactcacg caacctggaa
    10621 gccaggagcg tgttgcggcc ctcttccatg gtattcccgg ccgccatccc gattgccttc
    10681 cggctgccca ccagaaccac gacgcgtcgc cccctcgtga cggccgtata aagcagattc
    10741 ctgcgaagca tgacgaagtg ctgattgtgc atgcagacta ccaccgcagg aaactcgctt
    10801 ccctgggatt tgtgaacggt gacggcaaag gcctggcaga ggtcgtccat gtcagccgca
    10861 ggaatacgca cgattctcct gtcgaacctg acgagcagac cgttgtcgcc ggaagggccc
    10921 gcaacggtac cgatatcccc gttgaagaca tccaggtcat aattgtttct ggtctgcatg
    10981 accctgtctc ctggaaggaa gggcgtctgg gccgccagtt catcatccac gcctggattg
    11041 agcctgcgac ggatgcgttc attcaattcg tccgtcccgc aggatccctt tttcatcggt
    11101 gccaggacct ggatatcctg ccggggatca agcccgaagc gggccgggat gcgttcggtc
    11161 acgagcgcga cggcaaggtc ggcggcctcc tcggccgatt ccctgaacac cacgtggaaa
    11221 tcgcccgggt cggatccgga tgacctgtcg gacgaaggaa cccggccggc catgatttca
    11281 tgcgccgcgg aaacgatcga actgccgtcg ccctgacgga atacctgggt cagccttgcg
    11341 accgggacag atccagaccg gatcgcttcc ctgaaaggat cgcctggccc aacgggtgga
    11401 agctgatccg cgtccccgac aagggtcagc ctggtgcccg gcctgatggc tgcaacaagg
    11461 gcatgaaaca tgaacatgtc gatcatcgac gactcgtcaa ccaccaccca gtccgcctcc
    11521 agcgggttgt caggcccgcg gacgaaccgg tcggtggccg ggttgtactc aagcagccgg
    11581 tgcagcgtaa gcgcctccac tcctgaagcc tcgcgcatgc gcatggccgc tcggccggtg
    11641 ggcgcggcca gcgacacccg gattccggat ttccggcaga tggtcatcaa ccttccgaca
    11701 acggtagtct tcccggttcc cggtccaccg gtcaggatgg aaaccggaaa ggaacacagt
    11761 gttcgaagcg cttccatctg cgacggcgca agatcggact ccccgggaca ggcgacgaca
    11821 ggaatcgggg ccggcgcccc gctgcacagg gcggcaagcc cgaccgccgc gccaagttca
    11881 gcttggtgcg caccggacgc atagactaca gccccttcgc cggccctgtc ttcccgcacc
    11941 acttcgccgg ccgacaccag agcttccagc cctggcacga cctgccgagg cgtgagatca
    12001 accaggccgg acgccgactc cagaaggacg tcctcgcgaa ggaacatgtg tccggaatcc
    12061 tgggctccgg ccagcgcatg ggcgactgcg gccttcagcc tggcgggagc aagcggatcc
    12121 gtcccgatcc gggaagcaac gcgatccgcg gacttgaacc cgtacccgcg aagatgcctc
    12181 tgcaacgacc agggatccga agccacgatc gagggagccg acgcacccca gaggcccgcg
    12241 atcctcgacg cctgggccgg cagaagtccc atgtcgcaaa ggactatcac cgcctcgcgg
    12301 gcctcgatac ccttcttcca tgccttccag atggctgcgg cccgcccgga accaattccc
    12361 ggacaggaag tcaacttttc cggcccggtg tcaagcgcca ggcgcaggcc ttccccgaaa
    12421 tgatcaaaga gccgtctggc aaggatcttt ccaaccccct ccaccgcgcc tgacgataga
    12481 aaggccatta ttccccggcc ggaagacggg ggcacctgca tgcccgaccc gaaacggaac
    12541 tgaaccccga agcgcggatg attcctgaaa tccccgctca gccggacttc catgccgccg
    12601 gtcacgccgg ggaggggacc caccgcggtg acggtggtgc cagagatgtc ttcgggaagg
    12661 gaaacgatga cgtcgaacac ggaaaaaccg cgttcctcgt caaagtaccg aagttttgat
    12721 acctcaccgg aaagggttgt cacttcagac gtcttggtgc tgccctggcc aagtatagtc
    12781 caaatctccg ggatctggcc gacgtcgggc tcataactta gaatacccgc ggcagttttt
    12841 ccgttatgaa cgtgggcgtt gtctttcgcg gtcacgcccc ggaagtttta ttgcaggagc
    12901 cggaatgaaa aacctggtat cgacactgct tgttgtcctg tctgccctgg cgcccatggc
    12961 gcttgatgcc gaaccactgc cgctcgtgta cgaggccgtc actctggcga caacggacgg
    13021 caagcccaga atcgcggtgt ttcccagatc agtgccgctg cagatggcgc agggacgcac
    13081 ggccgacact ccatcggttc aaagaatctt tgaaacgatg aaggcccgct ttccggccgc
    13141 gtacggaacg accacgctgt cgatatccgg gggccgggag cccgtggcca cgattaacct
    13201 ggatccttcg agaaaggaaa cctgggggct cgtgacggcg gaaatcttct atacgctcaa
    13261 gtccttcggt ttcgaaaaca tcgtcgcgcc gctctatctg caggatccgc tgaacccatc
    13321 gctgatagat gccgtcgtca tggtgccggt tcagccctgg ttcaatgcag ccggggaaga
    13381 ctttccttcg acaattgcag tatcgctgga cggcagcagg atgatctcga caagcctttt
    13441 ccgccagctc ctgacgaccg gaaaccccga cgtcgcaagg atagtgcagg ccgcactgtc
    13501 ggcgcccctt gaagcgcaga ggcttgccgc ggtcagggcg ctggccgccc agaagccggt
    13561 atccgccgac ctcgtcgcaa ggatttcatc ccttctgaca gatccatcgc cggcaattcg
    13621 cggggcaatc ctggaactgg cggaggccag gaagatccag gttgccctgg aggatctccg
    13681 caaggtggct gacgccgatc aggatccggc gctgcggctg gccgcggcga agctgctttc
    13741 ggccgccggc gtcagggact acgaccacat cctcgaggtg cgaaaccttc gaagcggcaa
    13801 cccgaccgat atcgcgcagt cgcttcggcg cctgaagaca tcgggcaagt ccgacatcgc
    13861 tccagcggtc gcggaaacac ttgaatcccc ggacgagacg gtccgcaacc tggcggtgga
    13921 ggtactgctg tcgatgaagg ccgccaccgt ggtcgcggcg gcgctccaga acccgaaggt
    13981 tcagcccgca cagaaaacgg tcctggcttc gggcctgctt tcaatgtgtc ccggcgacga
    14041 ttcggcgatc gcctggatgg cgggacagcc tgccatggcc ggccgcgccc tcgccgccct
    14101 gtgcgggaca cccgcgcagc cccccgctta ctgtcgcagg tcatccggcg tccttcccgt
    14161 gatcccgttg ttcctgaaaa gacccgaaac cgaaatccgc ctgaaggcga ttgaatgcct
    14221 ttcgacacga aacgaacagg aagtcctgga tctcctgatc ccgatgacga cgacagcggg
    14281 caccgagcag gctgccacgg cggcagcgtc cgccgtattg tccaggcagc cgcaggcggt
    14341 tcttctcggg attatctccg gaaggggcgt gccggcagcg cgggccacga gacagtttcg
    14401 cctgctcgcg ctgaacggcc ttgcatcaca ggcacagtca acagcccttg aaccgtcggc
    14461 cctggccatc ctgaaaggtc tgatgaagga tcccgacata gagatccgca gggcggcgac
    14521 cggcgtgatg gcaagatcgc gcgacgcggc cgtcgcggcg ctggtgcttg acggcatcaa
    14581 cgatcaggac gagggaatca ggacggcggc cgtgatagct gccgggcgta tccccggacc
    14641 ggcttcggat gccgccatca ggaagtccct ggaagatctg tcggtccagg ttcgggcggc
    14701 cggacttgaa attctggcat caaggccgct tgaagggaca agaccgaaac ttcagatgct
    14761 ggcccagaac cagagacccg agatcaggcg gccggcagtc gcggccttcc tgaagacgct
    14821 cggccccggt gatgatgccg tcagaacgca gttcgtatcg acccttcttt atgacaattc
    14881 ggaagaaatc cggatcattg ccgtgcgggc catggaagag atgacggaca cccagtcggc
    14941 gcgggcggtc ggttcacttg tcatagaccg ctcggtgccc gtccgggttg aggttgcaag
    15001 ggttcttgga cagatgcgca acgacgtatc gctggattac ctgaagaaaa tggccaggga
    15061 tcaggacttc caggtcagga agactgtaat agaggccgtc ggacgcttcc ccgtcgcatc
    15121 ttccacgccg gcattccggg aaatgctggg aaccgaatcc gatggtaccc tgaaagcgct
    15181 gatcgagcag aaacttgcga gataggactg tccggaaaga aacgtcggac agaaccggtg
    15241 aatcaaggga taccggtcaa aagaaggcgt acccgaatcc aaattccacg acgaatggaa
    15301 agttcagatc caccagggcg ctgttggaaa cgaagtaacc ggagatcccc gcctgaacca
    15361 tgacgaaaac gcttcttgaa acgaacacgt tgacgcccag caaacctacc gccccgaaaa
    15421 ggaaggacgg ttcggtgacc tgggacgacg gacacagatc cggatcatcc ggccacagct
    15481 tgcatcttgg ttcggtggat gacttgaatc cactcaggta gtccatctcg aaaccgaaat
    15541 gcgtgaagac gcggccccag ttgaaaaccg ccgaataccc gacaccgatt gaggcccgga
    15601 agctgaagaa gtcgtccagc aggtcgccgt aggaatcctc tcgggcctga tagaaggaaa
    15661 gccggaggaa tgtgtcgagg cccggctgaa tctggtatgc ggtgccgatt gacaggccga
    15721 tattgtgaaa accggtcctg gtggaattgt cctgggcaaa catggaatag gaaaatccgg
    15781 tgtccaggaa aaccctgggg agtcttttca aggtgtcggt gcgggacgga agatcagcgc
    15841 acgcaagcca ggaagatatc gccatcgaca ccgactcgac gccgcggtac ggcctgctgt
    15901 ccctcatcac ctccaccgaa ccggcatcct tccgagaaac ctcgaaaacc ctcgtttcca
    15961 ggcgatcgcc gccttccgga tcgctttcca gaaaaacata aaccagtgcg tcggcaccgc
    16021 gttcacgaag gaatccaagc gcgcgttcgg tcgattcgaa cggattctcg cgcgccggcg
    16081 actttacgaa atcaagcgcc gccgcccgaa gagccctttc ctcccttgcc ggaaaccatc
    16141 cctgccggaa tctgcgttcc ggcatatgac cgaacatctg tttcagggca atgtgggcca
    16201 gatccggttc cgacgtctcc aggcgggaca gcccaaggta caggtaaaga tccgagacga
    16261 cggcaggaac caggacatcc gagtccgacg caagcgccgc cgatatgccg tcctccagcg
    16321 aggagatcgc ttccggcagg cggatatccc tgtacctctc gattcccaga tgcagcatgc
    16381 ccatcacgac caggtgacgg ctggcggttc cctggggggc cgaaaccggc tggcccgacg
    16441 acaggtcaac gagttcgatc tccggttccg cggcgagaac ggcggaagcc tcccttatca
    16501 attgcggcat ctcctgcaat ctcgggggtg gttccgaagg atcgaaaaac agcttgtccg
    16561 gatcgattcg aaagtccctt cccggtatca gaatcacctt ctttgatgcc gggcgcacga
    16621 cctcggaacc tgttaccgga actgccgtga tcagcccggc caggcagacg aggattccca
    16681 cgaatgctgc cctgcaagtc atccggcatc tccggtttca ggcgggtcgt cggcgtacag
    16741 aagcggatcc ccgcacttca aatcggcttc gccgacctgc ctccaggttc ttgcccttcg
    16801 aaaagtcaca tcgacctgac ctggctcgcc ggcatcctga agctcaagcg ttccggaaag
    16861 cagttcaccg gcttcgtcat cgacgaccac gggttccttg aacgtgaacc tgccgattat
    16921 caatggcgtc ccggacgaac cggggcagtc tccatggctc ctgaacgaca gcgccatgct
    16981 gcttgactcg actttcccgt tctcgacgaa ccgacacggg cagacttcgg aagacgggac
    17041 aaggaaatcg gcatcgtcat aaacacggac gataccggtg atgtcgggac cgaactgggc
    17101 aagcatcatc tcgaagtatc ggggcgtatc aaggccggga accccgtcaa tcaccactgg
    17161 cagatcggac tgataaatgc ccgaaatcat gcggcttgaa tcacatgaga ccaacccggc
    17221 caggaagagc gcagcggata aggcgggaca aactgccgaa aggcgaacgg acatcgggaa
    17281 acccccactc cggggaacat tcacccggga aggcctgacc ccccggcgtc cccgggttca
    17341 tcgccgtgat cggacggcga aggcgtgggc gtgggttctg aaacgacggc ggccccgggt
    17401 tcatcttcaa cgtcctcggc ctcgatgatc tcgccttcac cgacctcgac cgataacctg
    17461 gactcaagga cgggtctggg ctttccggtc gtcaaaaggc acagtcgatc ttccgccagg
    17521 tcaagctgca tctgggtgac aagccaggcc cgccggttgt gctcggcctt gcgcagggcg
    17581 acccggcgca tctcctcggc ttcggcgacc cttcttcgca tggtctcaag ttcggccttc
    17641 agcagcctgg catcgccacc gtcaggcact tcttcgaccg gtcgcggagc ggcctttgcc
    17701 gcacccgact cctcgagcct ggcaaggcgt gccttcagtc tctcgttttc ggccatgctg
    17761 gcggcaagtc gggccttaag ggactctgac tcttcggatg ccgccccggc agcgatcctg
    17821 gcttcctgcg cggccgccgc atgcatgtcg atctcgcgct tcagggtttc gtatttttcc
    17881 tcgtagaagg ccgccgcctt gtcggcacgc tcacgacagt cggccagtct caaggcctcg
    17941 gcctttgccg cggccacttc cgattgcagc gccgacgcgt cccgcgcggc gttcttcagg
    18001 tctgcgtcca ccgaagcaag cctggcgcgc atctggccaa tctcattctg gcaggcttcc
    18061 gcccgctgaa gggcaaggtc gagttccctg cggacatcct gaagttcttt atttccaaac
    18121 aagcccacaa atacctcccc aaggacggac ggcggacgcc ttcgtcagat gccgcagcgt
    18181 cgtcacgccg ggtccagatc gacaatctca aaatttccgg cgaccagatc ggcgcggtat
    18241 tccatcatcc ggaaggacag gttgccctcg cccggaacaa ccaccggaac cacgtgcgca
    18301 ccgagctcgc ttgaatccag cgtggcccag gcggcgatga tcaccttgtc gcccctgtgt
    18361 gccagatgag ccgccgcgcc gttgatctgg acgacccccg aaccgcgagg ccccggaatg
    18421 cagtaggtag aaaagcgcga gccggttgta acgttccaga cctcgacttc ctcgaccgga
    18481 gcaagccggg cggcgtccat caggtcgctg tcgattgtga tcgaccctac gtagttgacg
    18541 tcggcatcgg tgacggtcgc gcgatgaatc ttcgatctca ggaatctgcg aaacaacatg
    18601 attgcctccc ctgggtcaat ctgtcggcgg aatgctaccg tatcaaggcg tcgaagtaca
    18661 ggatcatatt agtctgttgt tgttgcgaag ccctgatttg atcgatacaa taccgcatgt
    18721 gcgccttttc catggagatc cggttattcc gggggccttg ggccggacgt tccggcaagg
    18781 acgaatgacg atgaacagat ccttactgca agccatcgcg gtggccggga tcgtgctggt
    18841 cgccggttgc ggcggtcagc cggaaaccca cgacgtgccg gtcgtcgata tccagattga
    18901 cagcatcacg gaacaggaca ccgccgtccc ggacgagggc gtgcagcgcc ccgacaacgg
    18961 aa
//
LOCUS       DAOPPA010000006        18901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_3546_length_18901_cov_4.345856, whole genome shotgun sequence.
ACCESSION   DAOPPA010000006 DAOPPA010000000
VERSION     DAOPPA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_18901_cov_4.345856"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..598)
                     /locus_tag="PKH54_00445"
     CDS             complement(149..598)
                     /locus_tag="PKH54_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOC98383.1"
                     /translation="MTARQFWKRRLTEYIRERGGRVTPARLQIAEVFFSMGSHPGIET
                     LSAEVKRRFPGIGEATVYRTMRLLCDAGLAGERQFGEGFSRFEPICGSAGDSHHDHLI
                     CVHCGAIVEFEDPGIERLQDRVAESHGFEITSHRLEIFGICGNCRGR"
     gene            complement(634..1524)
                     /locus_tag="PKH54_00450"
     CDS             complement(634..1524)
                     /locus_tag="PKH54_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98384.1"
                     /translation="MKRFVEFAAIVAVIVAVAGCSRKPPQKFVPDDPAPAIPALRTGP
                     AVAQAADSPAADTFSIVPEGTWKLSIDRQVFMRSTSMATTTVKSTTSLTMKVTDSTAA
                     SCSVSITDAESTFVALEPRDNIQEDVAATYRGLTFTIEPGIDGLVMIRPKDGRQVPEE
                     LSEALALMIPPFNPALMRPSESRAASRSVFPGLPGGRDDGGVLSGQWKLDDGAGGRGV
                     LALDFSAAVKGEATIESIHGRVTGGERRGRSLCAFGPTGLTGAGMAESGVYSVELKPR
                     KGKTRLLEQKVTVRASLGPA"
     gene            complement(1551..7253)
                     /locus_tag="PKH54_00455"
     CDS             complement(1551..7253)
                     /locus_tag="PKH54_00455"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC98385.1"
                     /translation="MQFRNLIVAFGLSLVIPGCTQLAPVADVPGCQDCPDGFLCVDAS
                     CVAGQDVVDDAGMDAHLELPDAGDPDAADTGDRVEADDGLTSDAADADSEESFETIED
                     TGTDDGEVVEYTVGNACESDLECIDGECVVMVSSGTEVRKVCTGPCGLKCPAGMACMN
                     HAITVDGFETRCEPFENGLCFPCESASDCLITGAQCLKMAGGNSFCTVPCEGADSRCP
                     YGFECKVLGDETILPEFRAQCQPAAGGCDCVFELAQARQPCRVVNDSGTCEGTLACYP
                     EVGWLPCSAAVPADETCDGKDNDCDGATDEDFRWTDWDQSGKEIGAACGIGACAGGKV
                     QCLGTSGATCSTAYLATTENCGSGSGNGRDDDCDGTTDEDCEIADADGDGVKAFEGDC
                     NDYDSAFYPGAREPCCPPAVASSPQAIARCDRNCDGAVTACDINDRDFDGYSNPHADP
                     AMRDCDDGNAAVHPGAPEKCDDGLDNDCMGDGDLHCCYADSEPGCVGPFDPDGDHYVG
                     SMDCDDQDPGRHPGAMEICDFIDNDCDGITDNGNPGGLAVDPSNPGGGTIAVNGGSRC
                     GLDEGECLAGIWVCVRYDTFAGLECIGSKPSKDEICDDLDNDCDGSTDEDFPGKGLAC
                     DGDDLDQCANGVNECNLAGTGIECIEPFALADLVDTCGDGIDQDCDGITDGNCFPTDL
                     DGDGYLPPADCDDTRSEVRPWAIEPCCDYRMSKEDALAACDWDCNGMTAKCNPVDYDF
                     DGAFDRDQGGTDCDEEDPTVRPGFPDKCGDSIDQDCSGVDTTCDSIVDRDGDRYASFE
                     DCDDNNAFRYPWAPEECNGIDDDCDGITDEGNPGTNPEVTCGSNEGICRIGAEVCVHF
                     KGTSPQIMCVPEVLPAKEMCDGIDNNCNGRTDEVFRDLGLPCDGPDSDQCRNGRFVCS
                     EDGSATVCGPELREDIVEVCDGIDNDCDGLTDEDFQHEGLAIGAECEGWGICGPGLVV
                     CSVDGVRATCSTNWDGPDSQAQPETCDGIDNDCDNRIDNGMMWYGLALGSLCRGIGEC
                     GIGHVECNPSTLKAVCSVNPDGSNPRDRREWCNGLDDDCDGRTDDNLVGTEWDCLRAG
                     VCRGLPIPAECVAGQWECDYTVVPEFQADETRCDNQDNDCDGQTDEGFGKGDACDGPD
                     YDRCKNGILVCKADGTGVECGPETIENITEKCNRIDDDCDGRTDEDFPIGELCDGPDN
                     DQCKNGSFTCSADTLGVVCNNEWIKDIVETCNQVDDDCDGLTDEDFAVGLPCDSDDPD
                     LCAFGKTVCNQAGNGVVCHEDPEQQATTEKCNGVDDDCNGQTDEGFLYLGTAVGGDCD
                     GIGQCGAGKVVCTADSVKATCSTNADGTTPQAVAEACDMLDNDCDGSTDEGLTWRGIA
                     MGQPCVADGNCAGGTVVCSPTDLVATCSTMPNGTEPGATTEKCDNIDNDCDGFTDEDI
                     PQDLSSCRTAGICLPSLVTSACVGGSWACNYSAVAGFEQGAETKCDGLDNNCDGNTDE
                     TWSVGQACDGPDDDLCKNGVMVCNPDGKNASCSSETGSFVETCGDSVDNDCDGATDEE
                     GSSGCKPYWFSGDGDLFGVGSSRCLCYSGQVTGFVATAGGDCNDSNPNIYPGATELCN
                     YKDDDCDGQTDKEPQFLLLGGSCDSSDPDTCANGMWVCSPDGTALVCQDDYNSAEKCN
                     GVDDDCNGQTDELWQLGQPCDGPDSDLCQNSTWICKPDGSDAQCVEAISNITERCNGK
                     DDDCDGFTDETFTTLGLPCDGSDPDSCANGTYTCKANGSGVECVNEPGTFPSGTEYCD
                     GFDNNCNGSADENWPNKSKRCDSSDTDTCRTGINLCNGSQTGIECVGDVVCATGATCT
                     DSGSEYLPDRCLCGLPPVVCSRTQGSTCTAGVCKCGNGAACSGAQQCLSDGLGGYACQ
                     "
     gene            7401..8795
                     /gene="mnmE"
                     /locus_tag="PKH54_00460"
     CDS             7401..8795
                     /gene="mnmE"
                     /locus_tag="PKH54_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOC98386.1"
                     /translation="MAEEFGDVIVAPASGAGSCAVSVIRLSGAGSARLVSLLCEGGCD
                     EGHDQASGRRDRFLRLAWLRHPSSGRRLDQAMVVTFAAGASFTGEESAEIFCHGGPVI
                     VDSIVRACLDLGARHAGPGDFTRRALASGRIDLVQAEAVSLLSGSESDAATDVGLEAL
                     AGRPSEQVSALSERLLDILAEYDASMDFDSSDGVYVDLSPLPAMLSEVAATMDSWLES
                     ARAVRPAVTGFRVVLAGPPNVGKSTLFNAIAGSDRAIVHERPGTTRDVITERLVLSGV
                     GCLLFDTAGIRNSDDDIEAEGVNRAFSAAASADLLLHVVDCAGNSPALPKLLSVTVSG
                     AASHALVLARADLCARPPEIAAPGIPVFVVSARTGQGIPELTAFISDQARKAVASASR
                     VSAVVSGERQVQAVAMAQDSVRKAVEAGMSSAPAEVTASLVRMAVQSLGEVTGASITD
                     QVLDRIFSRFCIGK"
     gene            complement(8935..9285)
                     /locus_tag="PKH54_00465"
     CDS             complement(8935..9285)
                     /locus_tag="PKH54_00465"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC98387.1"
                     /translation="MAAEKRTQSRFHTRIFCVFKNDLNLDETEVLMLNMGSGGAFINT
                     DSPAPPGTPVTLRMYLKPDSEPLSISAEVVWWRTNAREGTPGMGVKFSRVGKDDLEAI
                     KAFIENLIEDDLFQ"
     gene            complement(9373..10110)
                     /locus_tag="PKH54_00470"
     CDS             complement(9373..10110)
                     /locus_tag="PKH54_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC98388.1"
                     /translation="METREYELMAAAEDVHPWFIQLRTIIRESFESVGVRPEWHVLDV
                     GCGTGGTMKSLAGTCRFTGLDASPLAAEMAERISGAKVVVADATRMPFADRTFDAVIA
                     SHILEHIDDDGAAVAEIRRVLKPGGPLVVLVPCHQFMYNHHDRALHHVRRYSRRDFIE
                     LLEQNGFTPQKIIWTNPIMYPPTAAARIVSRILRPADGAGDGRGSDTTARLGLASHLV
                     SAIASIEVRLMWKIPAPFGVGLLVLAR"
     gene            complement(10274..10469)
                     /gene="ssrS"
                     /locus_tag="PKH54_00475"
     ncRNA           complement(10274..10469)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKH54_00475"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(10532..11824)
                     /gene="purD"
                     /locus_tag="PKH54_00480"
     CDS             complement(10532..11824)
                     /gene="purD"
                     /locus_tag="PKH54_00480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOC98389.1"
                     /translation="MKILVVGNGGREHAMARKLAQSPGATVFITTGNAGTDEVATPIP
                     IKPTDVDAIVRFAAAERFDLVAVGPEIPLAAGLADRLVEAGVTVFGPVAAAARLEASK
                     AFSHEIMDAAGVPSARTHTFTDLDAALDHLSTCPVPVVVKADGLAAGKGVVVAMTRDQ
                     ALDAARSFMADRTMGDAGDMLVIEEFLQGEEASFMCLTDGRTIRPLASARDHKRIFDG
                     DRGPNTGGMGAYSPTPLVGPDNSPELVEKVMRPVIDELARRGIEYRGVLYAGLMMTAD
                     GPKVLEFNCRFGDPETQPVLTRFRGDLAATMIATATGRLEEAVFDFDSRVAVCVVAAA
                     PGYPGKVSTGQSISGLRDAASIPDVFIYHAGTSKGPDSMPVVSGGRVLAVTALADDFA
                     AARELAYRAMDRIDFAGIQFRRDIAASVSTHRENTGKP"
     gene            complement(11876..13420)
                     /gene="purH"
                     /locus_tag="PKH54_00485"
     CDS             complement(11876..13420)
                     /gene="purH"
                     /locus_tag="PKH54_00485"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOC98390.1"
                     /translation="MKRAVISVSDKTGVVDFARGLVALGYQIISTGGTSKTLREEGLQ
                     VTDVSDVTGFPECLEGRVKTLHPAVMAGILARDTEDHRKTIADLGIEMIDIVAVNLYP
                     FQATIAKAGVTEAEAIEKIDIGGPSMIRAAAKNSDRVTVIVDHADYKPVLEELARDGK
                     TSDQTRRRLMVKVFAMTAAYDMAVAGHFGSIDGALPDVAFAGGVKVSELRYGENGHQR
                     AAVYVPAGTVPKGMIGARQLQGKELSYNNYVDMDAAWALVREFDDRPAVSIIKHTNPA
                     GFGWDRESLHHAYEKALATDPVSAFGGIVALNREVDEKLAERLNEHFYEVIICPSLTA
                     GARDFFAKKKNLRVMEMGDDFFQPMPDLVFKQVSGGMLVQDNDPPANEVSGARVVTKR
                     QPTREEWDALECAWKVSKHVKSNAIVFCFNDRTGAVGAGQMSRVDSARLAVMKAADRK
                     IDLNGTVVGSDAFFPFADGVEEIAKAGATAIAQPGGSIRDQEVIDAADRMGLAMVFTG
                     TRHFRH"
     gene            complement(13463..14299)
                     /locus_tag="PKH54_00490"
     CDS             complement(13463..14299)
                     /locus_tag="PKH54_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98391.1"
                     /translation="MNVFKVGILLVMAVAATALMSAGCSRKAAEPAPAAEPAPAAEPA
                     PAPVAVQPPAEVKAPEPVWPSPTPEHWLIVNDYEKPADLVEAPINPKTGKPGKKENLN
                     RNLQDGEWGDVGFNGGKCRLEQVRDGQNNVLKVTYSMPTETSECGTYEYLLPYVEKKV
                     KGKLIKNYPQFFDLRPYDRIAAMVKSGDDKEHNLKLHLTELDPYGSQLQGYVSPTKNI
                     VAGKDWQRVEFKISDTLHEFFDPAHAKAIGLRIRFQEQDNRNDAGIVLFDNITLIKKT
                     GE"
     gene            14377..15570
                     /locus_tag="PKH54_00495"
     CDS             14377..15570
                     /locus_tag="PKH54_00495"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOC98392.1"
                     /translation="MKTVTTHQIRKLLWLVLDSVGIGALPDAHLYGDEGSNTLLHSAL
                     AVGDFRMPHLARLGLGHLVDVPGIPREPGPDGHFARLAERSPGKDTTTGHWEMAGLVM
                     KGAFKAFTDTGFPPDVIAEFERRSGFKTIGNYSASGTDIIRDLGPEHERTGSLIVYTS
                     ADSVFQIAAHEDVVPLERLYSACMVAREILDAHNVARVIARPFVGSPGSYTRTYNRRD
                     FSMTPPGPTVLDMLSGAGIPVVGVGKIHDIFAGRGVTESIHTEGNRDGLIQSIRQIRD
                     LDRGLVFTNLVDFDMVWGHRRNAEGYAAGLMEADSFVPGLMEAVGPEGMLIVTADHGC
                     DPTAAWSTDHTREYVPFLAWHHGIAPGSGRDVGPRTTFADIGATVAALFGVGPTVDGE
                     PMDLS"
     gene            complement(15572..16744)
                     /gene="hisE"
                     /locus_tag="PKH54_00500"
     CDS             complement(15572..16744)
                     /gene="hisE"
                     /locus_tag="PKH54_00500"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOC98393.1"
                     /translation="MIVSFFKIDSRQFDTSEATRLAQSIEEAGRVGRALLFFPEGIPD
                     SPSFLPWLKGLCRAVVIAVRGGISTPDPVETLVRAGVTTVVIEEATPFETLSRMPRDR
                     TVVALDPDNYRQYADVSAVVQKMTDKMNSLAPWVSGFMIDVDWSPEEVNQLPLDTIRR
                     FRDAAGSCLWVRHPTLTADDTPAFDELGIDAVVHDGDIRVYADAFIRSLNFHEGLIPT
                     VAVDKYGQVLRVAWSSPRSLSDSIIRGTSGYCGRNGVHSDLSTGVGRLLRVIPDEERA
                     ALMFVVRPPEQNGTRFSYSSFGPGGREFGLQRLFDIIHGLKERPRPGSYTSFLFERED
                     RIPRKLNEEVRELLEAGTRDEIAWEAADVLFFLFAYLAGTDVPLDDVVAELMGRKQ"
     gene            16876..18360
                     /locus_tag="PKH54_00505"
     CDS             16876..18360
                     /locus_tag="PKH54_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98394.1"
                     /translation="MSRELLVCIWTACLAAMVSCTAADNVTDSVVEPDVPVGTRCVSS
                     FQCDDDDPCTVERCDRGLCRIDPIVCDDNDTCTTDSCADGGCVFERTPGCCGSDDDCD
                     DGDPCTKDDRCDLKTRTCRIADPIEPCCRSHLDCDDGDVCTVDTCQENLLCSHRWDKS
                     GSGAGICCTLETDCADANRCTRDFCRITDESASPPTGVCAREILCCKRDRDCQQTDAP
                     CMQGVCVIPSGQDDGECDYQRIPDCCETSSDCVAEPCLSGTCDDGNCSFDAIPGCCTD
                     DEQCGDPCLFCNRGALPSGTCERRTDSNCCESVVYFHDFEGDFGMEVVPMPGPGYSPS
                     PEWHVSTRRSGSGTSSMFFGDEETGLCRGDSSMKTGSRAVTGPIKLMMTRSPHLRFMM
                     WKNTDEFSRFNDVVSVYVRTTGGETKVFSTSQMEKFNSGDGFVEFPPAAESAVSLEQW
                     DDQVVQLVFEYDSVVYASAPYEGIYIDDIVVSGTCDDRGVALHD"
     gene            18353..>18901
                     /locus_tag="PKH54_00510"
     CDS             18353..>18901
                     /locus_tag="PKH54_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98395.1"
                     /translation="MTDSSNGVPDHMDDADGPPDSMPDPGGDRDEGVESPPDSPGWER
                     TRSTIQLKAMQAAVQLGDAVGGTGEAISSRVRNDAGRVRRVTDLFSQGVVGLMLALHL
                     GGTAVLSGVVFAEAFPYAYMYVFMGLLLWLVLTMRFLALKAGRRAAAFRSLVVNLLLT
                     AFWTYVLLDQIPGRPVVEGALRF"
BASE COUNT         3220 a         5992 c         5773 g         3916 t
ORIGIN      
        1 gaacccgcga cctacagctt gggaagctgt cactctacca ctgagttaat cccgcggcac
       61 ggaaacaggg cgattttcca cgccggcgca cttcgtgtca agcgatccgt cgtcacggtc
      121 gccggatccc gtccgcccgt cacgtcgatc acctgccgcg gcaattcccg cagatgccga
      181 aaatttccag ccggtggctg gtgatttcaa aaccgtggga ttcggcgaca cgatcctgca
      241 ggcgttcaat cccaggatcc tcgaattcga cgatggcgcc gcagtggacg cagataagat
      301 gatcgtggtg ggagtcgccg gcagagccgc atatcggctc gaagcgtgaa aacccttcac
      361 cgaactggcg ttccccggcc aggcccgcgt cgcacaacag ccgcatcgtc cgatagacgg
      421 tggcttcgcc gatgcctgga aaccgtcgct tcacttcggc tgaaagggtc tctatgcccg
      481 ggtggctgcc catggaaaag aatacttccg cgatctgaag ccttgcgggc gtgaccctgc
      541 cgccacgttc ccttatgtat tcggtaaggc gccttttcca gaattgcctt gctgtcatgc
      601 ttccttcaga ccccgtgtcg ccaaggcccg caatcaggca ggtcccaggg acgcccttac
      661 agtgaccttc tgttccagaa gcctggtctt gcctttgcgc ggcttcaatt cgaccgaata
      721 gacgccggat tcggccatgc ccgcgccggt cagtccggtc ggcccgaacg cgcacaggga
      781 tcggccccgt cgttcgccgc cggtcaccct tccgtgaatc gactcgatgg ttgcctcgcc
      841 cttgaccgct gccgagaagt caagcgccag tacaccgcgc ccgccggccc cgtcatcgag
      901 tttccattga ccggaaagca ccccgccgtc atcccttccg cccggcaggc ctggaaagac
      961 gctgcggctg gccgccctgg attcggaagg ccgcataagg gccggattga aaggcggaat
     1021 catcagggcc agggcttcgg aaagctcttc cggcacctgc cgtccgtcct tcggccgaat
     1081 catgacaaga ccgtcgatcc ccggttcgat tgtgaacgtc agtccgcggt aggtggccgc
     1141 gacgtcttcc tgaatgttgt ccctgggttc aagcgcgacg aaggtggatt cggcatcggt
     1201 tatcgaaacg ctgcacgacg cggccgtcga gtcggtcacc ttcatggtca ggcttgtcgt
     1261 ggatttgaca gttgtcgtcg ccatgctggt cgaacgcatg aacacctgtc ggtcgatcga
     1321 aagcttccac gtaccttcgg gaactatgga aaaggtgtcc gcggccggcg aatcggccgc
     1381 ctgtgccacg gccggccccg tccgcagggc tggaattgcc ggtgccggat cgtcggggac
     1441 gaatttttgc gggggcttgc gggagcaacc cgcgactgca acgatgacgg cgacaatcgc
     1501 cgcgaattca acgaaccgct tcataagggt gccttcccgg gtgttcaaga ttactggcag
     1561 gcatatccgc caagaccgtc cgacaggcac tgctgcgcgc cggaacaggc ggcgccgttg
     1621 ccgcatttgc agacgccggc ggtgcaggtc gatccctgcg tcctgctgca aacgacgggg
     1681 ggcagcccgc aaaggcacct gtcgggaagg tattcggatc ccgagtcggt gcaggtcgcg
     1741 ccggtcgcgc acaccacgtc gccgacgcat tctatcccgg tctgcgaccc gttgcacagg
     1801 ttgattcccg tccggcacgt atcggtatcg ctggagtcgc accgctttga cttgttcggc
     1861 cagttttcgt cagcacttcc gttgcagttg ttgtcgaacc cgtcgcaata ctcggttccg
     1921 ctggggaagg taccgggctc gttgacgcac tcgacgccgc ttccgttggc cttgcacgtg
     1981 taagtcccgt tggcgcaact gtcgggatcc gatccgtcgc agggcaggcc gagcgtcgtg
     2041 aaagtctcgt cggtgaaccc gtcgcagtcg tcgtcctttc cgttgcacct ttccgtgatg
     2101 tttgaaatcg cctcgacgca ctgggcgtcc gacccatccg gtttgcaaat ccaggtactg
     2161 ttctggcaca ggtcgctgtc ggggccgtcg caaggttgac caagctgcca cagttcgtcg
     2221 gtctggccgt tgcagtcatc atcgacgccg ttgcacttct cggcactgtt gtaatcgtcc
     2281 tggcagacaa gcgcggttcc gtcgggcgaa cacacccaca tcccgttcgc gcaggtgtcc
     2341 gggtcgcttg aatcgcacga accgcccagt aaaaggaact ggggctcctt gtcggtctgg
     2401 ccgtcgcagt cgtcgtcctt gtagttgcac aactcggtcg cgccgggata gatgttcggg
     2461 ttcgaatcgt tgcagtcgcc gccggcggtc gcaacgaacc ccgttacctg tcccgaatag
     2521 cacaggcacc tcgaggaacc gacgccgaaa agatccccgt ccccgctgaa ccagtaaggc
     2581 ttgcagcccg acgaaccttc ctcgtcggtc gctccgtcgc agtcgttatc gaccgagtcg
     2641 ccgcaggttt cgacgaaact gccggtttca ctggagcaac tggcgttctt gccatcgggg
     2701 ttgcacacca tgacgccgtt cttgcacaag tcgtcgtccg ggccgtcgca ggcctggccg
     2761 accgaccagg tttcgtcggt attgccgtcg cagttgttgt ccaggccgtc gcacttcgtt
     2821 tcggcgccct gttcgaatcc ggccacggcc gaatagttgc atgcccacga accgccgacg
     2881 catgccgacg tgacaaggga aggcaggcat attccggccg tccggcacga cgacagatcc
     2941 tggggaatgt cctcgtcggt gaatccgtcg cagtcgttgt cgatgttgtc gcacttttcg
     3001 gtcgtcgccc ccggctcggt tccgttcggc atcgtcgaac acgttgccac caggtcggtt
     3061 ggcgaacaga ccaccgtccc gcccgcgcag tttccatccg cgacgcaagg ctggcccatc
     3121 gcgattccgc gccaggtaag cccctcgtcg gtacttccgt cgcagtcgtt gtcgagcatg
     3181 tcgcaggcct ctgccaccgc ctgcggggtg gtgccgtccg cattggttga acaggtggcc
     3241 ttcaccgagt cggccgtgca cacgactttc cccgcgccgc actgtccgat tccgtcgcag
     3301 tcgcccccga ccgcggtacc caggtaaagg aagccttcgt ccgtctgtcc gttgcagtcg
     3361 tcatcgactc cgttgcactt ttcggtcgtg gcctgctgtt ccggatcctc gtggcagacc
     3421 accccgtttc cggcctggtt gcacacggtc ttcccgaagg cgcacagatc cgggtcgtcg
     3481 ctgtcgcacg ggagtccgac ggcgaaatcc tcgtcggtca gcccgtcgca gtcgtcgtca
     3541 acctggttgc acgtttcgac gatgtccttg atccattcgt tgttgcacac gacgcccagt
     3601 gtgtccgccg agcacgtgaa cgatccgttc ttgcattgat cgttgtcggg gccgtcgcaa
     3661 agttcaccga tcgggaagtc ctcgtcggtt cttccgtcgc agtcgtcgtc aatcctgttg
     3721 cacttttcgg tgatgttctc gatggtctcg ggcccgcatt cgacaccggt gccgtcggcc
     3781 ttgcacacga gtattccgtt tttgcagcgg tcgtagtccg ggccgtcgca tgcgtcgccc
     3841 ttcccgaaac cttcgtcggt ctgtccgtcg cagtcgttgt cctggttgtc gcaccgcgtt
     3901 tcgtcggcct ggaattcggg cacgaccgtg tagtcgcatt cccactggcc cgccacgcat
     3961 tcggccggga tcggcaggcc gcggcagacg ccggcccgca ggcagtccca ctcggttccc
     4021 accagattgt cgtcggtcct gccgtcgcag tcgtcgtcaa gcccgttgca ccactcgcgc
     4081 ctgtcccgtg ggtttgagcc gtccggattg accgagcaga ccgccttcag tgtcgaaggg
     4141 ttgcactcca cgtgcccgat gccgcactcg ccgatgccgc ggcagaggct gcccagcgca
     4201 aggccgtacc acatcatgcc gttgtcgatc cggttgtcgc agtcgttgtc gatgccgtcg
     4261 caggtttccg gctgtgcctg gctgtccggt ccgtcccagt ttgtcgagca tgtcgcgcgg
     4321 acgccatcga cgctgcacac gaccagcccg ggtccgcaga tgccccaccc ttcgcattca
     4381 gcaccgatcg ccagtccttc gtgctggaaa tcctcgtcgg taaggccgtc gcagtcgttg
     4441 tcgataccgt cgcagacctc gacgatgtct tcccgcagtt ccggcccgca gacggtcgcg
     4501 cttccatcct cggaacacac gaacctgccg ttcctgcact gatccgagtc cggtccgtcg
     4561 cacggcagtc ccaggtcgcg gaagacctcg tcagtcctgc cgttgcagtt gttgtcaatc
     4621 ccgtcgcaca tctcctttgc cgggagaacc tccgggacgc acatgatttg aggcgaagtg
     4681 cccttgaaat ggacgcagac ctcggcgccg atccggcaga tcccttcgtt gctgccgcac
     4741 gtgacttccg gattcgttcc cggattgcct tcgtccgtta tcccgtcgca gtcgtcgtcg
     4801 atgccgttgc attcctcggg agcccaggga tacctgaacg cattgttgtc gtcgcagtct
     4861 tcgaacgatg cgtaccggtc gccgtcgcga tcgacaatgg agtcgcatgt cgtatcgacg
     4921 ccggaacagt cctggtcgat gctgtcgccg cacttgtccg ggaagcccgg ccttacggtc
     4981 ggatcttcct cgtcgcagtc ggttccgccc tgatccctgt cgaaagcccc gtcgaagtcg
     5041 taatcgaccg ggttgcactt ggccgtcatc ccgttgcagt cccagtcgca ggcggcaagc
     5101 gcgtcttcct tgctcatcct gtaatcgcag cacggttcga tggcccacgg ccttacttcg
     5161 gatctggtgt cgtcgcagtc ggccggcggc agatagccgt cgccgtcgag gtcggtcggg
     5221 aagcagttcc cgtcggtgat tccgtcgcag tcctggtcga ttccatcacc gcatgtatcg
     5281 acaagatcgg ccagcgcgaa cggctcgatg cactcgattc cagttcccgc caggttgcat
     5341 tcgttcacgc cgttggcgca ctgatccagg tcgtcgccgt cgcacgccag gcccttgccc
     5401 gggaagtctt cgtccgtcga accgtcgcag tcgttgtcca ggtcgtcgca gatttcgtct
     5461 ttcgacggct tcgagccgat gcattcgagg cccgcgaacg tgtcgtaccg cacgcagacc
     5521 catattccgg ccaggcattc gccttcgtcc agcccgcacc tggaaccgcc gttgaccgcg
     5581 atcgtcccgc cgccgggatt ggagggatcg accgccagcc cgcccgggtt gccgttgtcg
     5641 gtgattccgt cgcagtcgtt gtcgatgaag tcgcatatct ccatggcgcc gggatgacgt
     5701 ccgggatcct ggtcgtcgca gtccatcgag cccacgtagt ggtcgccgtc gggatcgaac
     5761 ggcccgacgc agcccggttc cgaatccgcg tagcagcagt gcagatcccc gtcgcccatg
     5821 cagtcgttgt ccaggccgtc gtcgcatttt tcgggcgccc cgggatgaac ggccgcgttg
     5881 ccgtcgtcgc agtcccgcat cgccggatcc gcgtggggat tcgagtagcc gtcaaaatcc
     5941 cggtcgttaa tgtcgcacgc ggtcaccgcc ccgtcgcagt tccggtcgca cctggcgatg
     6001 gcctggggtg aggatgccac ggcgggcgga cagcacggct cgcgcgcgcc ggggtagaaa
     6061 gccgagtcgt agtcgttgca gtcgccttcg aaggccttga cgccgtcacc gtccgcgtcc
     6121 gcgatttcac agtcctcgtc ggtggttccg tcacagtcgt catcgcggcc gtttcccgat
     6181 ccggatccgc agttttcggt ggttgccagg taagccgtgc tgcaagtggc ccccgacgtt
     6241 ccaaggcatt gaaccttgcc gccggcgcag gccccgatgc cgcaggccgc gccgatttcc
     6301 ttgccggact gatcccagtc ggtccagcgg aagtcctcgt cggtggcgcc gtcgcagtcg
     6361 ttgtccttac cgtcgcaggt ttcgtcagcc ggcactgccg ccgagcatgg aagccagccg
     6421 acttccggat aacaggccag ggttccttcg caggttcccg aatcgttgac aacgcggcag
     6481 ggctgtcgtg cctgggccag ttcaaagacg cagtcgcagc cgccggccgc gggctgacac
     6541 tgggcccgga attccggaag tattgtttcg tcgccaagaa ccttgcattc gaatccgtat
     6601 ggacaccggc tgtcggcgcc ttcgcacggc acggtgcaga agctgtttcc gcccgccatc
     6661 ttcaggcact gggcgccggt tatcaggcag tcggaagccg attcgcacgg gaagcacagg
     6721 ccgttctcga acggctcgca ccgggtctcg aatccatcaa ccgtgatcgc gtggttcatg
     6781 cacgccatgc cggccggaca cttcagtccg cacgggccgg tacagacctt ccggacttcg
     6841 gttccgcttg aaaccatgac gacgcattcg ccgtcgatgc attccagatc gctttcgcac
     6901 gcgttgccga cggtgtattc gacaacttcg ccgtcgtcgg ttccggtgtc ctcaatcgtt
     6961 tcaaaagact cctcggaatc cgcgtcggcg gcatcggatg tcagaccgtc gtcggcttca
     7021 acacggtcgc cggtatcggc ggcgtccgga tcgccggcgt ccgggagttc cagatgggcg
     7081 tccattccgg cgtcatccac gacatcctgt ccggcaacgc aggaagcatc gacgcaaagg
     7141 aatccgtctg ggcagtcctg acaacctgga acatctgcga caggcgccag ctgcgtgcac
     7201 cccggaatca ccagggacag cccgaaggcg acaatcaagt ttctgaattg catcggatat
     7261 tcccgtttcg aaacctttat ggccgcacaa ttctatcaga aataacctgt tccaaggaaa
     7321 ctgcaatata gtcatgtcat cccgattcgg gtcggcggcc gttaccgaag gccgtccgcc
     7381 gtgccctgga tggaaggaaa ttggcagaag aattcggcga tgtgatcgtt gccccggcct
     7441 caggcgccgg aagttgcgcg gtttcggtca tcaggttgtc cggcgcaggt tcggcccgcc
     7501 tggtttccct tctttgcgaa ggcggatgcg acgagggaca tgaccaggct tcgggccgac
     7561 gcgacaggtt tcttcggctg gcgtggcttc gtcacccgtc atcgggacgc aggcttgacc
     7621 aggcgatggt cgtgactttc gcggccgggg cctcgttcac cggcgaggag tcggccgaaa
     7681 ttttctgtca cggcggtccc gtcatagttg attcaatcgt ccgggcgtgc cttgaccttg
     7741 gcgcccgtca tgccggcccg ggcgatttta cccgccgggc ccttgcatcc ggccggattg
     7801 atctcgtgca ggccgaggcg gtatccctgc tttccggctc cgaaagcgat gcggcgaccg
     7861 acgtcggtct ggaagcgctg gccggtcgcc cgtccgaaca ggtttcggcc ctttccgaaa
     7921 ggcttctgga cattcttgcc gagtacgacg cgtcgatgga tttcgattcg tcggacgggg
     7981 tttacgtcga tctttcccca ttgcccgcga tgctttccga agtggccgcc accatggatt
     8041 cctggcttga atcggcgcgc gcggtcaggc cggccgtaac cggtttccgg gtggtgcttg
     8101 ccggtccgcc gaatgtcggg aagtcaactc ttttcaatgc gattgccgga tccgaccgcg
     8161 cgatcgttca tgaaaggcca ggcaccacaa gggacgtcat caccgaaagg ctggtgcttt
     8221 caggcgtcgg ctgtctgctg ttcgacacgg ccggtatccg caactcggac gacgacatcg
     8281 aggccgaagg cgtcaatcgc gccttcagtg ctgccgcctc ggccgatctt ctgcttcacg
     8341 tcgtcgattg cgccgggaat tcgccggctc ttccgaagtt gctgtcagtc accgtcagcg
     8401 gcgccgcatc ccacgccctg gtgcttgcca gggccgacct ttgcgcccgt ccgcctgaaa
     8461 tcgccgcccc cgggattccc gtgttcgtgg tcagcgccag aaccggacag ggaatccccg
     8521 aactgacggc gttcatttcc gatcaggccc gcaaggccgt cgcttcggca tccagggtat
     8581 cggccgtcgt atccggcgaa cgccaggttc aggcggtggc gatggcgcaa gacagcgtgc
     8641 ggaaggccgt cgaggccggc atgtcgtccg cgccggccga ggtgaccgcg tcgctggtca
     8701 ggatggccgt gcagtcgctg ggagaggtca cgggggcgtc catcacggat caggttcttg
     8761 atcggatttt ttcacggttc tgcatcggca agtgacggcc tgtcccgggt cgtttcgcgc
     8821 agcaggcata caggttgatg gagtcggtgc cgcgccggac gggcaggttc gacgccgttg
     8881 cggcgggcga ttgttttcac ggttcggtgg cgcgccgaat tggcgtggct ggtttcactg
     8941 gaacaggtcg tcttcgatca ggttttctat gaatgccttg atggcttcga ggtcgtcttt
     9001 tccaaccctg gaaaacttca cgcccatccc gggggtgccc tccctggcgt tcgttctcca
     9061 ccagacgact tcggcgctta ttgaaagcgg ttccgagtcc ggtttaaggt acattcgaag
     9121 cgttaccggg gttcccggcg gcgcggggct gtcggtgttg atgaatgcgc ccccggaacc
     9181 catgttcagc atcaggactt cggtctcgtc caggttcagg tcgttcttga aaacgcagaa
     9241 aatccgcgtg tggaatcttg attgagttct tttttcggct gccaagatga acccctttgc
     9301 gagtcggccg ccatcttccc gtgggtggcc ggtcaggcac atcggcaagc ataatccacg
     9361 caggccgtct tatcaacggg ccagtaccag cagcccgacc ccgaacgggg ccgggatctt
     9421 ccacatcagg cgcacctcga tcgatgcgat cgccgaaacc agatggctcg caagcccgag
     9481 tctggccgtc gtatcgctgc cacgcccatc gcccgcgccg tccgccggcc ggagaatcct
     9541 cgaaacaatc cgtgccgccg cggttggggg atacataatc gggtttgtcc agatgatttt
     9601 ttgcggcgtg aacccgtttt gttcaagcag ttcgatgaaa tccctgcgtg agtaacgcct
     9661 gacgtgatga agcgccctgt cgtgatggtt gtacatgaac tggtggcacg gaaccagcac
     9721 caccagtggt ccgccgggct tcagcacgcg gcggatttcc gccaccgccg cgccgtcgtc
     9781 gtcgatgtgc tccagtatgt ggctggcaat caccgcgtcg aacgtccgat ccgcgaaagg
     9841 cattctggtt gcgtcggcca ccacgacctt cgcgccggat atcctttcgg ccatttccgc
     9901 cgcaagcggg ctggcgtcaa gtccggtgaa tcggcaggtc ccggccaggc tcttcattgt
     9961 tccgccggtt ccgcagccga cgtcgaggac gtgccattcc gggcggactc cgaccgactc
    10021 aaagctttcc cggattatcg tcctcaactg gatgaaccag gggtggacgt cttccgccgc
    10081 cgccatgagc tcgtattccc tggtctccat ccagcccccc ggaattgatt tggataccgg
    10141 cccctcccgg gccagggcag atcactgctg caaggcgcag gatatcacga acgcaaaatg
    10201 gcccgccatg tatagatgag atccccggaa tccatcagcg gccgcgtcgg gccgtccggg
    10261 aagtggtggg tggaggtgcc ccgcctgaat cgtctggaga caatgaattg aaacccattt
    10321 tccaaacagg tggggtgtca cggtaaccgc ttcaggcttc ctgccgggat tggctggcat
    10381 gcacacggcg gtcaggggcg acgcccggat ttcaaagcaa gggattcaac caaaaaagtg
    10441 attgcctccc gcgcattccg cagggcacct gaagccagta taagaccttg ttcccgatga
    10501 tgcaaaccgg ttcggggaaa tgcaggttta atcagggttt gccggtgttt tcgcgatgcg
    10561 ttgaaacgga cgccgcgata tcgcggcgga actggattcc cgcgaagtcg atccgatcca
    10621 ttgcccggta cgcaagttcg cgggcggcgg cgaagtcgtc cgccagcgcg gtaaccgcaa
    10681 gcacccgtcc cccggagacg accggcattg aatccgggcc ctttgaagta ccggcgtgat
    10741 aaatgaagac gtcgggtatc gatgcggcgt cccgaaggcc ggaaatcgac tggcctgtcg
    10801 agaccttgcc cgggtagccc ggcgccgccg cgaccacgca aaccgccacg cggctgtcga
    10861 aatcgaaaac cgcttcctcg agcctgccgg tggccgtcgc gatcatcgtc gcggccaggt
    10921 cgccgcggaa ccttgtcaga accggctgcg tctcggggtc cccgaacctg cagttgaact
    10981 cgagcacctt cggcccgtcc gcagtcatca tcaggccggc gtaaagaacc ccgcggtatt
    11041 cgatcccgcg tcgcgccagt tcgtcgataa cgggccgcat gaccttctcg acaagttccg
    11101 ggctgttgtc cggcccgacc agcggagtcg ggctgtatgc ccccatcccg ccggtattcg
    11161 gtcccctgtc gccgtcgaag atgcgcttgt ggtcgcgtgc cgaggcaagc ggcctgatcg
    11221 tcctgccgtc ggtcaggcac atgaacgagg cttcctcgcc ctgaaggaat tcctcgatca
    11281 ccagcatgtc gccggcgtcg cccatcgtcc tgtccgccat gaaggatctg gcggcgtcaa
    11341 gggcctgatc cctggtcatg gcgacgacga ccccctttcc ggcggcaagt ccgtcagcct
    11401 tgaccaccac cggcaccgga catgtggaca ggtgatcaag ggccgcgtcc agatcggtaa
    11461 aagtatgcgt ccgcgccgaa ggcacgccgg ccgcatccat gatctcgtgg gaaaacgcct
    11521 tcgaagcctc gagccgggcc gctgccgcga ccggtccgaa aaccgttacg ccggcctcga
    11581 cgagcctgtc ggcaagcccg gcggccaggg ggatttccgg accgacggca acgagatcga
    11641 acctttcggc ggcggcgaag cggacgatgg catcgacgtc ggtcggcttg atcgggatcg
    11701 gtgtggcgac ttcgtccgtt cccgcgtttc cagtggtgat gaagacggtt gcccctggac
    11761 tctgggccag cttccgggcc attgcgtgct cgcgcccgcc gttaccgaca accaggattt
    11821 tcatgcccgg gactccttca gcaaaagaaa tggcatggat ctcgtccggc atccgtcagt
    11881 ggcggaagtg cctggtgccc gtgaagacca tggcaagccc catcctgtcg gccgcgtcga
    11941 tgacttcctg atcacggatt gaaccgcccg gctgggcgat ggccgtggcc ccggccttcg
    12001 cgatttcctc gaccccgtcg gcgaacggga agaaggcgtc ggaaccgacc accgtgccgt
    12061 tcaggtcgat cttgcggtcg gccgccttca tgacggcaag cctggcggaa tcgacgcggc
    12121 tcatctgtcc ggccccgacc gcgccggtcc tgtcgttgaa gcagaacact atcgcattgc
    12181 tcttgacgtg cttcgaaacc ttccatgcgc actcgagggc gtcccactct tcccgggtgg
    12241 gctggcgttt cgtcaccacg cgggcgcctg aaacctcgtt tgcgggcggg tcgttgtcct
    12301 gaacgagcat gccgccgctg acctgcttga aaaccagatc gggcatcggc tggaagaagt
    12361 cgtcgcccat ttccatgacg cgaaggttct tcttcttcgc gaagaagtcg cgtgccccgg
    12421 cggtcaggct ggggcagatg ataacttcat agaagtgctc gttcaggcgt tcggccagct
    12481 tttcatcgac ttcccggttg agcgccacga ttccgccgaa agcggaaacc gggtcggtcg
    12541 ccagggcctt ttcatacgcg tggtgaaggc tttcccgatc ccagccgaag ccggcggggt
    12601 tggtgtgctt gatgatgctg acggccggcc tgtcatcgaa ctcgcgcaca agcgcccagg
    12661 cagcgtccat atcgacgtaa ttgttgtacg aaagttcctt tccctgaagc tgtcgcgcac
    12721 cgatcatccc cttcgggacg gttcccgccg gcacatacac ggcggccctc tggtggccgt
    12781 tttcaccgta acgcaactcg ctgaccttca ccccgcccgc gaaggcgacg tccggcagcg
    12841 cgccgtcgat actgccgaag tgtcccgcga ccgccatgtc gtatgcggcc gtcatggcga
    12901 ataccttcac catcagccgt cggcgggtct ggtcggacgt ctttccgtcc ctggccagtt
    12961 cttcaagcac cggcttgtag tcggcgtggt caacgatgac cgtcacgcga tccgaattct
    13021 tcgccgccgc cctgatcatg gacgggccgc cgatgtcgat cttctcgatg gcctcggcct
    13081 cggtcacgcc ggctttggcg atggtcgcct ggaacgggta aaggttgacc gcgacgatgt
    13141 cgatcatctc gatgccgaga tccgcgattg tcttcctgtg gtcttccgtg tccctggcga
    13201 gaatgcccgc catcaccgcg gggtgaagcg tcttgacccg gccttccagg cactccggga
    13261 atcccgtcac gtccgaaacg tccgtcacct gcaggccttc ctcgcgaagg gtcttcgagg
    13321 tgccgccggt cgaaattatc tggtagccca gggcaaccag gccacgtgcg aaatcgacca
    13381 cgcccgtctt atcggaaaca ctgattactg cgcgtttcat tcaaaaaacc tcgctgacgc
    13441 atgaaaaaga tggacagtcc ggctactcgc cggtcttctt gatgagagtg atgttatcga
    13501 acagcacgat gccggcgtcg ttgcggttgt cctgttcctg gaacctgatc cgcaggccga
    13561 ttgccttagc gtgggcgggg tcgaagaatt cgtgcagggt gtcggaaatc ttgaattcca
    13621 cgcgctgcca gtcctttccc gcgacgatgt tcttcgtcgg gctgacatat ccctgcaact
    13681 ggcttccgta gggatccagt tcggtcaggt gcagtttcag gttatgttcc ttgtcgtcgc
    13741 cggacttcac catcgccgcg attctgtcgt acggacgcag atcgaagaac tgcggataat
    13801 tcttgatcag cttgcccttg accttctttt cgacgtacgg caacaggtat tcgtaggtac
    13861 cgcattccga ggtttcggtc ggcatgctgt aagtgacctt caggacattg ttctgtccgt
    13921 ccctgacctg ttccaggcgg cactttccac cgttgaaccc gacgtcgccc cactcaccgt
    13981 cctgcaggtt ccggttcagg ttttcctttt tgcccggttt gccggtcttc ggattgatcg
    14041 gcgcctcgac aaggtcggcc ggcttttcgt aatcgttgac gatcagccag tgttccgggg
    14101 tcggcgacgg ccacaccggc tcgggagcct tgacctcggc gggcggctgc acggccaccg
    14161 gggccggtgc cggttcggcg gccggtgccg gttcggcggc cggtgccggt tcggcggcct
    14221 tccgggaaca cccggccgac atcaacgccg ttgcagcaac agccatcacg agaaggatcc
    14281 cgaccttgaa aacgttcatc tgagcctcct gcaaaacttt ccggtcaacg ccgggcggcc
    14341 ttgaccacct cgaatcatgg cacgattata tggggcatga aaactgtgac aacccatcaa
    14401 attcgcaagt tgctctggct ggttctcgac agcgtcggga tcggcgccct gcctgatgcg
    14461 catctttacg gcgacgaagg ctccaacacg cttcttcatt cggcactggc tgtcggcgat
    14521 tttcggatgc cgcacctggc gaggctggga cttggtcacc ttgtcgatgt gcccggcatt
    14581 ccccgggagc ccggaccgga cggccacttc gcccgtctgg ccgaaaggtc gccgggaaag
    14641 gacaccacga ccgggcactg ggaaatggcc ggcctggtga tgaaaggggc attcaaggcc
    14701 ttcaccgata ccggatttcc gcctgacgta attgctgaat ttgaaaggag aagcggtttc
    14761 aaaacgatcg ggaattattc ggcgagcggc accgacatca tccgggatct ggggccggaa
    14821 cacgaaagga ccggcagtct gatcgtgtat acatcggccg attcggtgtt ccagattgcc
    14881 gcccatgagg acgtggttcc ccttgaacgt ctttattccg cctgcatggt cgccagggaa
    14941 atactggacg cccacaacgt cgccagggtc atcgccaggc cgttcgtcgg cagtcccggt
    15001 tcctatacaa ggacttacaa ccgccgcgac ttctcgatga cccctcccgg cccgacggtt
    15061 ctggacatgc tttccggcgc gggcatcccg gtagtcggag tcggcaagat tcacgacatc
    15121 tttgcgggaa ggggcgtcac cgaatcgatt cacaccgaag gaaaccgcga tgggctgatc
    15181 cagtcgatcc ggcagatcag ggatctcgat cgtggactgg tttttaccaa cctggtcgat
    15241 ttcgacatgg tatggggcca tcgccgcaat gccgaaggat acgccgccgg cctgatggaa
    15301 gccgattcgt tcgttccagg gctgatggag gccgtcggcc cggaaggcat gcttatcgtt
    15361 accgcggatc acggatgcga cccgaccgcg gcgtggtcaa ccgatcacac cagggaatat
    15421 gtgccattcc tggcctggca tcatgggatt gcccccggtt ccggtcgcga tgtcggcccg
    15481 aggacgacgt tcgccgatat cggcgcgacc gtcgcggccc ttttcggcgt cggcccgacc
    15541 gttgacggcg agccgatgga tctttcctga tttattgctt gcggcccatc agttccgcaa
    15601 cgacgtcgtc gagcggaaca tccgttcctg cgaggtaggc gaacaggaag aacaggacgt
    15661 ccgccgcttc ccaggctatc tcgtcccttg tgccggcctc cagcagctcg cggacttcct
    15721 cgttcagctt gcgcggaatg cgatcctcgc gttcgaacag gaaggacgtg tacgaacccg
    15781 gcctcggtcg ttccttcagt ccgtgaatga tgtcgaatag ccgctgcagg ccgaattcgc
    15841 gaccgccggg accgaaggac gagtacgaaa atctggttcc gttctgttct ggcggcctta
    15901 cgacgaacat cagagccgcc ctttcctcgt ccggaattac ccgcagcagt cggccgacgc
    15961 cggtcgaaag gtcgctgtgg acgccgttcc gaccgcagta tccggacgtt cccctgatga
    16021 tgctgtccga caggctccgg ggagacgacc aggccacccg cagaacctgg ccgtacttgt
    16081 cgacggccac ggttggaatc aggccttcgt ggaagttcag gcttctgatg aaggcgtcgg
    16141 catacacccg aatgtcgccg tcgtgaacca ccgcgtcgat tccgagttcg tcgaatgccg
    16201 gcgtgtcgtc ggcggtcagt gtcgggtgcc ttacccacag gcaggaaccg gcggcgtcgc
    16261 ggaagcgtcg aatcgtatcg agcgggagct ggttgacctc ttccggggac cagtcaacgt
    16321 cgatcatgaa gccggatacc catggggcca gggagttcat cttgtcggtc atcttctgca
    16381 cgacggcgct gacatccgcg tactgccggt agttgtcggg atccagcgcc acgaccgtcc
    16441 gatcccgggg catcctggac agggtctcga aaggcgttgc ttcttcaatg acgactgtcg
    16501 tgacgcccgc acgcaccagc gtctcgaccg ggtcgggagt gcttattccc ccgcgcacgg
    16561 cgattacgac ggcgcgacac aggcctttca gccacggaag gaacgacggg gagtccggga
    16621 tgccttccgg gaagaaaagc agcgccctgc cgacgcgtcc ggcttcctcg atggactggg
    16681 caagccttgt cgcctccgag gtgtcaaatt gtcgggaatc gattttgaag aacgaaacaa
    16741 tcatctgaaa tcaccgggtt ttccaggaac ggccggcagc tttcaccaaa agatgcgcga
    16801 ttcggtgctg ccagggcaca cgtggtatta tcacatccgt ttcaagttgt aacctcggat
    16861 cgggagggcc agggcatgag tcgtgaattg ctggtctgca tatggacggc atgcctggcc
    16921 gcaatggttt cgtgcactgc ggccgacaac gttaccgatt cggtggttga accggacgtg
    16981 ccggtcggaa ccaggtgcgt cagttcattc cagtgtgatg acgacgaccc atgcactgtc
    17041 gaacgctgcg acagggggct ttgcaggatt gatccgattg tctgcgacga caacgacacg
    17101 tgcacaacgg attcctgcgc cgacggcgga tgcgtcttcg aaaggacgcc gggctgctgc
    17161 ggttcggatg acgactgcga cgacggtgac ccatgcacga aggacgaccg gtgcgacctg
    17221 aagacgagga cgtgccgaat cgcggatccg attgaaccat gctgccgttc ccatctggac
    17281 tgcgacgacg gtgacgtctg caccgtcgat acatgccagg agaatctcct gtgttcgcac
    17341 aggtgggaca agtcgggttc cggcgccggg atctgctgca cgcttgaaac ggattgtgcc
    17401 gatgccaaca gatgcaccag ggatttctgc cggataaccg atgagtccgc gtcgccgccc
    17461 accggcgtct gcgcccgcga aattctctgc tgcaagcgcg acagggactg ccagcagacc
    17521 gatgccccat gcatgcaggg cgtctgcgtc ataccgtccg gccaggacga cggcgagtgt
    17581 gactatcaac ggattccgga ctgctgcgaa acgtcgtccg actgcgtggc cgaaccgtgc
    17641 ctttccggaa cctgtgacga cggaaactgc agttttgacg cgattcctgg ttgctgcacg
    17701 gacgatgaac agtgcggaga cccatgtctt ttctgcaaca ggggtgccct gccgtccgga
    17761 acgtgcgagc gcaggaccga ttccaactgc tgcgagtctg tcgtgtattt ccacgatttc
    17821 gaaggcgatt tcggcatgga agtcgttccg atgccgggcc ctggttattc gccgtcgccg
    17881 gaatggcacg tctccacgcg ccggtccggt tccggaacca gttccatgtt cttcggggat
    17941 gaggaaaccg gtctgtgtcg cggcgattca agcatgaaaa ccggttcaag ggcggtgaca
    18001 ggcccgatca agctgatgat gacaagatcg ccgcatctgc ggttcatgat gtggaagaac
    18061 actgacgagt tcagcaggtt caatgacgtg gtttccgtat atgtcagaac cacgggtggc
    18121 gaaacaaagg tgttttcgac cagccagatg gagaaattca actccggcga tggatttgtg
    18181 gagttcccgc cggccgctga aagcgcggtt tcccttgaac aatgggacga tcaggtcgtc
    18241 cagcttgttt tcgaatacga ttcggtggtt tatgcttcgg ccccctatga aggcatttat
    18301 attgacgaca tagtggtttc ggggacttgt gacgatcggg gagtcgctct tcatgactga
    18361 ttcttcaaac ggggttccgg atcacatgga cgacgcggac gggccgccgg attccatgcc
    18421 cgatccgggc ggcgaccggg acgaaggcgt ggaatccccg ccggacagtc ccggctggga
    18481 aaggacaagg tcaaccatcc agttgaaggc gatgcaggcg gcggtgcaac tcggtgacgc
    18541 ggtgggcggc accggcgagg cgatttcgtc cagggtcagg aacgacgccg gtcgcgtccg
    18601 tcgggtcacg gatcttttca gccagggcgt tgtcgggctg atgcttgccc tgcatcttgg
    18661 cggaaccgcg gtcctgagcg gggtcgtgtt tgccgaggcc tttccatacg cgtacatgta
    18721 cgttttcatg gggctgcttc tgtggcttgt gctgaccatg cgtttcctgg cgctgaaggc
    18781 cggtcggcgc gcggccgcat tccgctcgct cgtggtcaac ctcctgttga cggcgttctg
    18841 gacgtacgtg ctgctcgacc agattccggg gcggccggtg gtagagggcg ccctccggtt
    18901 c
//
LOCUS       DAOPPA010000007        18056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_3728_length_18056_cov_4.008888, whole genome shotgun sequence.
ACCESSION   DAOPPA010000007 DAOPPA010000000
VERSION     DAOPPA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_18056_cov_4.008888"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKH54_00515"
     CDS             complement(<1..670)
                     /locus_tag="PKH54_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOC98396.1"
                     /translation="MYTTPGFDNEKYLNEQTAAILERAEMFGNKLYLEFGGKLIFDFH
                     AARILPGFNPNVKMHLLQKLKDQAELIVCIYAGDIERRKVRADFGITYEFDALKLIDD
                     LRDRGILVRGVVITRFDEQPAACAFRDKLERRGVPVFMHRHTKGYPTDVDLIVSSSGY
                     GANPYIETTRPLVIVTGPGPGSGKLATCLSQVYHEHARGVRAGYAKFETFPIWNLPLQ
                     HPVNV"
     gene            898..1104
                     /locus_tag="PKH54_00520"
     CDS             898..1104
                     /locus_tag="PKH54_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOC98397.1"
                     /translation="MATNLALDDELIEAVKSAGGHKTKKAAVTAALLEYLKVRERMKI
                     IELAGTVDFDPNYDYKTARRSKQA"
     gene            1101..1529
                     /locus_tag="PKH54_00525"
     CDS             1101..1529
                     /locus_tag="PKH54_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOC98398.1"
                     /translation="MRVLIDTSVWSQFLRRPASPATPDQDRLRMVVADLIADGRVVLP
                     GVVRQEILSGIRDDARFHKVKTVLRTIDDEPMTTEDFESAAAAFTTCRRAGIAPTFVD
                     MTLCALAIRLQAPILTLDKDFARYATVLPIQIHDCMTRMP"
     gene            complement(1534..2859)
                     /locus_tag="PKH54_00530"
     CDS             complement(1534..2859)
                     /locus_tag="PKH54_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC98399.1"
                     /translation="MWRSAEIELTRWKESPTRKPLIVRGARQVGKTWTIKAFGRAYYD
                     NTSVIDLERDASARAIFDGDLGASALIPQLEAFCGNPLSGGRTLLFIDEIQAQPRALT
                     ALRYLYEERPDIPVIAAGSALEFALGEVSFPVGRVSFLRMHPMTFHEFLVARGLDMLS
                     AALPTLGSPHTASDAVHMKLLGELRTYLLVGGMPEAVSGFIAENSLAAAARVHSDLAS
                     SLAGSLARYQRRADRESLETVFSSLPGAVGGQVIYSHIDPARRTEVTKASVQILARAL
                     LVTPVRDTAGDALPLGAGASDKRFKPLFLDVGLLQYLLGVSASVLFSGNDLSAVHRGA
                     VVEQFVGQELLASGGSENGSMYYWSRAGQRGNAEVDFIMSRGGKVYPVEVKSGAAGRM
                     RSANLFLDSHPNSPAALVFSPLASAKRQEGRLIYTPIYTRFDDSGLVVA"
     gene            3008..3328
                     /gene="cutA"
                     /locus_tag="PKH54_00535"
     CDS             3008..3328
                     /gene="cutA"
                     /locus_tag="PKH54_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012350817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOC98400.1"
                     /translation="METVIAMTISSFPDRERALECARALIERKVAACVQVTSPITSVY
                     TWDSKTCEDDEVLLFVKTTLDRTEEAIRQIKTMHPYDVPEIITLPVIDGFGGYFDWVR
                     DSVK"
     gene            3410..5683
                     /locus_tag="PKH54_00540"
     CDS             3410..5683
                     /locus_tag="PKH54_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98401.1"
                     /translation="MVHRSLANIFIIAALFAFGCGGGPTPQSDVVQDTTEADTVSDTA
                     LPDIDQADVPSDDSNSDIPSDIALPDGLERPVADPVAAARAFRLYYAERVDRAVVAFN
                     RFMLFGDTMFGINIRKVGVARSGDTFEVVPGPKDNNQIGTSVRAAWNAYRLYRTRTLE
                     LALIRMFNGMEFIGTVSGHDGVTGRNAYPNWTMTINGPAGTVGRTRDGTTVESPYVSD
                     AGLESEMISTFFGDMDVTYRLEPADILFNYMPAVETGSYAVTYSFSMLPDFLRISDCC
                     TSMMKVPDDYPWAGAFWSNHNSRDNFPDLVFGYLAAGEAMDDPDAGPELKAAATAAWE
                     AGRKVGDLVQQNDSRIMTVSEHTTYDQLTVSGEMRPDGTVEAETLGAMSDCQMNYAAR
                     ALSTDGLDAQLPELPLPGALENLIAPFVDVESGCEWMEGGHTCTRLAESFCGKTWGQL
                     NELTMGGKGLIDLAMELDEEDPGAAGAILGQFYGNYDQPMNAALAVVEYARLTGKQDL
                     LELAQATLAEMTDLSRTFADVIFGRADPAEQAQMRYETALIDGLGGLTPPATDFLDFA
                     VAEQQMAAMEGLLEMPDSTVAALLTEQEIADQIDDEQSDASAPVKKRYRDNWGDIPPV
                     RVEGEGYQAKVFERDTASDWRPVERPHHRRMGGIDLLEALPLCVTNPQVLDCTWAKAG
                     CARPDLYHDGKVDDADRTIFQTTAQGTTGCLPANNYCDGADLDHTGTVDALDTAFMDA
                     AQGCHYFLSSQPSDNAR"
     gene            complement(5786..6820)
                     /gene="cydB"
                     /locus_tag="PKH54_00545"
     CDS             complement(5786..6820)
                     /gene="cydB"
                     /locus_tag="PKH54_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOC98402.1"
                     /translation="MMDLQALQVIWFILYFVLIAGYAILDGFDLGVGALSLFAKDQRE
                     RGIHMDSIAPVWDGNEVWLLTAGGALFAAFPFVYATVFSGFYLALMLLLVALIWRAVS
                     FEFRNKVHSPGWIRAWDWAFGLGSLLPPVLFGVAVGNILRGIPLTEKTEFAGTFLGLL
                     NPYAVLVGVLALVMFLAHGAIYLVLKTDGDLQARMQRWAPGLWIGWVVLYFAATVASL
                     FVSPFLFDGILKNPVFWILFPLMMACVVLIPVLLKSGRHMLAFIASSAAIATQIGLAA
                     LGLFPRLVPALNDPKLSLTIYNASSTQRTLTTMLMIALVGVPIVIGYTIFIYRVFKGK
                     VRPGELYGNH"
     gene            complement(6817..8142)
                     /locus_tag="PKH54_00550"
     CDS             complement(6817..8142)
                     /locus_tag="PKH54_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOC98403.1"
                     /translation="MDALILARLQFAMTIGFHFLFPPISIGLAWLLVFMEWRGWRTGD
                     QVWVRMGKFFGKLFALTFAVGVATGIVMEFQFGTNWASYSRFVGDIFGAPLAAEGVFA
                     FFLESSFLGLYLFGRNKVGKGVHWFSILMVAVGATISAFWILVANSWQQTPAGFLVVD
                     GRAVLTSFSDAVFNPSMWYRFFHTLVACLITGAFFVGGVAAARLLRDNQEEVSRRALK
                     IAVIAGFIFAAMSAFPTGDSHAKQVAQTQPEKFAAFEGIYKTQKGAPLVAFGIPNAAE
                     QRLDYAIEIPKLASLLAFGDPEAEVKGMDAFPKDELPPVGLTFFSFHTMVMLGLLFIG
                     TMALGLLLIWRQKLDISRWYHRLVLLVAPLTLVASQVGWITAEVGRQPWSVYRLLKTA
                     DSVSITVGVPELWFSIALIGLMYVLLGGLYIFMLVREVKHGPEKAPEVA"
     gene            complement(8235..8540)
                     /locus_tag="PKH54_00555"
     CDS             complement(8235..8540)
                     /locus_tag="PKH54_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98404.1"
                     /translation="MRRVFNWIVFGALAGVAVATAFAPVVLRTLLASTGAKDAMCQCV
                     ELVDNTARLLIKTQVWGLVIGAVLFPIAAWAIRRAWNKRRAARAIPTQTTPEQQNTK"
     gene            complement(8571..9542)
                     /locus_tag="PKH54_00560"
     CDS             complement(8571..9542)
                     /locus_tag="PKH54_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC98405.1"
                     /translation="MIFGRLRIPRGALVLIGVMLASVPAMAQEAPDLSRLADFVRWGG
                     VALSVLVIVGTMVVLRLVQNIANRLGIRFANRRPAIQKLESVARFVLYIAAAIICVAL
                     SFRLDSTAVTVIGGALAFAVGFAMRDLVAAFLAGITIMVDRPFQVGDRVSYAGEYGDI
                     IKIGLRSVQMNTLDHNIVTIPNNKILTDVTSSGNYGSLEMQVPMDFFIGADQDVGKAV
                     ALVQEACLTSPYVFLDRPVPMRVKQVLLDNMVAVRVRARPYVYDSRHEKAFETDVHLR
                     VMDAFARNRIMPPAILHRSANVSLESVGNADGGMVAVGGISGAGLPD"
     gene            complement(9544..10488)
                     /locus_tag="PKH54_00565"
     CDS             complement(9544..10488)
                     /locus_tag="PKH54_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98406.1"
                     /translation="MLAIVGERGAGKSRLLQRIRDGHPLSTLVECPVDGIEGLRATLA
                     VALGLDPAASIQDCAVAAESRGDDSAILIDHANRLVLPVMGGLDGFDKFAGIARRHSE
                     RTTWILVFNEVIWQFFERSRGAYPVFDEVVRIEPWREEEIIALLRARSAQVHMEPSFE
                     AVIDRPIRDGDSIDREEAVSRVARGYFRMLWDYADGNPGVALDVWRRQLGAGLDGKVF
                     VRPFQVPDVRELERLSDEDVFVVRAIIQLEPAAVDDVVKASTRSQGRVLDLLRYGTAR
                     GWFEPVGDRYRVSWNWYRPVTRFLQRRHLLASFHRFGG"
     gene            complement(10400..12511)
                     /locus_tag="PKH54_00570"
     CDS             complement(10400..12511)
                     /locus_tag="PKH54_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98407.1"
                     /translation="MLTELLMTGRHGASIHGFIAGQRLVACLVCIGIVLTAPPVLADD
                     ASGRSADVIQTRSDLDSSDDDASSRDVRAPDAANEAAGDALPGGDPGVAVESDSAGVT
                     GRDMRDVIDRASAAVDEMIQVRPDPEAQAVEVLRQKAAEAAALARVEAQRAVSAEFDE
                     LEIISARQAKFRKELAGRLEILDLRLEQVPGFRRLVRELRELKAPDAKSADALYDDLR
                     SFLRSSRDELADALRELTADSAVPSIGSSIIQDVPPGVDTSTLEQARRKVVQTAVELA
                     SDEQEYRQKRAAQLYEEVRALNADRLALLSILSEKRRAEITGFTRAGLDQAVAELRQV
                     TLVLRYHLAATAAWFITLADGSVDRSDSLLATGTSAVKLLGLILLFWWWRRRSPELFD
                     AWIERIVQRDRAARIPQVSAIHRLVVFVARARRPIEWLAFILVLVWVLPPVVRTRLEV
                     VILKTVFVWTLCGAFVIGAIDALVAWPTVSRSGSGGTAATDAIRLRSLKLAGYTTVIL
                     GLILTIGSQLVGKGTIYRWVIWVCWFSVIPVILVIIRWWRDVIFDRIQASRRRTSFQD
                     WVAQRRKGWKSFPAAVSGGIYLFISGAYWIIRGWITGFDLARRAHAWFFRRGMDRMAG
                     DQESGTVLMPIDPDVFDRLGPDRSSGTLISGASYARVMEIVKKNRVKGRRFACHCRRA
                     RRGEVPASAAHQGWPSFINAG"
     gene            12770..14266
                     /locus_tag="PKH54_00575"
     CDS             12770..14266
                     /locus_tag="PKH54_00575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOC98408.1"
                     /translation="MTKTETESERRERRLAAEHAVWRILASADRLEGVVPSILKEVCR
                     AGAWALGELWTPGDDGQILHLTDVWNDTLFDLDRFVEVSWVTTFQSGIGLPGRVLETG
                     APTWIPDVAADPNFPRLKLADEAGLHAAFGFPIRIHGQVIGVMTFYSPDFRQPDPELL
                     ESLDVIGGQIGHFIERARTFQALAESEARWQRILSGAPFGIFHSTLDGKLLDVNPAFA
                     TMLGFESPEEAISAVNRTSIPQALYVVQAERDDFLRRLREQPGSWVSAEVQGRRTDGT
                     TVIGELHVKWLYEYFGHSPAMEGFVTDITERKRMERELRSALDRVRELTLRDHLTGLH
                     NRRHLEDRLAIEIAAARRTGDDISVIMFDIDFFKKINDTHGHQIGDVALQALAVTLAA
                     DVRPEDVLARYGGEEFVLLLRQTGPREAWEAAERLRRMVMTRPIAVPFGEIRMTISCG
                     VASMSECGARDQDSLMHAADQRLYQAKAQGRNRTSGAGVLEDIPDFVL"
     gene            complement(14334..15782)
                     /locus_tag="PKH54_00580"
     CDS             complement(14334..15782)
                     /locus_tag="PKH54_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOC98409.1"
                     /translation="MKFSADTKINDLLNAYPFLEDFLASYESKFEMLRNKMTRATIGR
                     IATVRTAAGIANIDLDALVAAIAAEIARHTGAFPEIGESTAPAQTRESRLAALKEVIS
                     DLHAGGDLEAARQKFAAAVEDVEATEIAAMEEELIRGGLPVSEVQRLCDVHVGAFREA
                     LDGHAELQAPPGHPVDTYMAANKVISTLANRLGEIARQVSGSGNAAAALSSAIGILDG
                     LAGLENHYQRKENQLFPLLERYKVMGPSQVMWGVHDQIRAAMKKVRAAAVAGDAATFA
                     ADAPALARDIVEMIYKEEKILFPMAMQTFTDDEWAEVRHGEDELGYVLATPAAPFPAK
                     RHVAAPAQVRLSGLAGLMTGDIDMEQLNLILCNLPVDVSFVDEADTVRFYSEGPDRIF
                     PRSPAVIGRKVQFCHPPKSLNVVQDILDSFRAGRQSVAEFWLQLGPRFIHIRYFALRD
                     KAGTYRGCLEVSQDVTGIRALQGERRLLEWKS"
     gene            15967..16320
                     /locus_tag="PKH54_00585"
     CDS             15967..16320
                     /locus_tag="PKH54_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC98410.1"
                     /translation="MKKALEPGMPAASAIEIGALVEYQQDSIVSRTLVKHNGGTLTLF
                     AFDEGQALSEHTAPFNAIVQVLDGTVELVIGGKPVPAVAGQTVLMPANIPHAVNAIKP
                     FKMLLTMIRDVQTTA"
     gene            16344..16667
                     /locus_tag="PKH54_00590"
     CDS             16344..16667
                     /locus_tag="PKH54_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOC98411.1"
                     /translation="MQATRGLRESRTVLAMIRIFCRGCHGTDGRNLCGNCDELFRYVQ
                     ARIEKCRYDDDKPTCLNCRVHCFKPAMREQIRLVMRYAGPRMPLRHPYLTLMHILDGH
                     RKPPP"
     gene            16739..17596
                     /locus_tag="PKH54_00595"
     CDS             16739..17596
                     /locus_tag="PKH54_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOC98412.1"
                     /translation="MNKTFLRDNLKRLVLVLLGSFLAALGVQLFLVPIHLLTAGIAGF
                     ALLLSYVTPIPAGLGLFILNVPIFLFCMKYLEKEFLVWSIVGMITLSASFALTAPLAD
                     LHPVKDMYLNLIAGAVVSGFGCGLVFRARASQGGTDVIAAAVRKVSSVRIGYLLFVLN
                     ASVVVILAIMYGLEPALATIVVIAIESVVIERTIIGVDGNKALMIITRKPDEIAAELM
                     KRVDRGVTIIEASGAYTHERRPIVLCILRTRQLAIATKIVRDIDPESFTYVHDVTEVI
                     GHGFKASPI"
     gene            complement(17609..>18056)
                     /locus_tag="PKH54_00600"
     CDS             complement(17609..>18056)
                     /locus_tag="PKH54_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC98413.1"
                     /translation="VYRKLVKKDGRLVGILLLDFIPWPWAGGGSNFFNRLQAKVDKLG
                     IWGAGLLGLVFALTFCPLSAAIFFGSLVPLAVKHESSVMMPAVYGIGTALPVIVFSVI
                     LAFAANRLSKAFNAMTIIEIWMRRLTASVFLLIGGYYCLVYLFRIL"
BASE COUNT         3986 a         5523 c         5231 g         3316 t
ORIGIN      
        1 ccacgttgac cggatgctgc agcggcaggt tccagatcgg gaacgtctcg aactttgcgt
       61 acccggcccg cacgccgcgg gcgtgctcgt gatagacctg ggacaggcac gtcgccagct
      121 tgccgctgcc tggaccgggg ccggtcacga ttaccagcgg acgcgtggtc tcgatgtacg
      181 ggttcgcgcc gtaaccgctt gagctgacga tcagatcgac gtcagtcgga tatcccttgg
      241 tgtggcggtg catgaacacc ggcacgccgc ggcgttccaa cttgtcgcgg aaggcgcagg
      301 cggcgggctg ctcgtcgaac ctggtgataa cgacgccgcg aaccaggatg ccacggtcac
      361 gcaggtcatc gatcagcttc aacgcatcga attcgtacgt aatgccaaaa tcggcacgaa
      421 ccttgcgccg ctcgatgtcg ccggcataga tgcagacgat cagttcggcc tggtccttca
      481 gcttctgcag caggtgcatc ttcacgttcg ggttgaaccc gggcaggatg cgggccgcgt
      541 ggaagtcgaa tatcagcttg ccgccgaact ccaggtaaag cttgttcccg aacatctccg
      601 cgcgctcgag gatggcggcg gtctgctcgt taaggtactt ctcgttgtca aatccagggg
      661 ttgtgtacat gtttgctctt gtcccgggga agcccgggcg ttctcaggct ggaattgttt
      721 tgtacgccga ttcccgtttg gacgcaacgg acgattcgac ccaacgccaa cctttgaaca
      781 gtttgtatcg actgaccgtt ttaagaaacg aagacgcctt ccaatcgata gatgcttgtg
      841 ccccgttctt catttcacac acttaattgt gtagaatagt gtgtggaggt ggcgatcatg
      901 gcgacaaacc ttgcactgga tgacgaattg attgaagcgg tgaaatcagc gggcggacac
      961 aagaccaaaa aggctgcggt tacggcagcg cttctggagt acctgaaagt ccgcgaacgc
     1021 atgaaaatta ttgaactcgc tggaaccgtg gacttcgacc ccaactacga ctacaagact
     1081 gctcgccgga gcaaacaagc atgagggtgc tgattgacac atcggtgtgg tcccaattcc
     1141 ttcgccgccc cgcttcgccg gccacgccgg atcaggacag actgcgcatg gtggttgctg
     1201 acttgattgc ggacggacgt gtcgtgctcc ccggggttgt gcgccaggag attctttccg
     1261 gaataaggga cgacgccagg ttccacaagg tgaagaccgt cctgcgcacc attgacgatg
     1321 aaccaatgac gaccgaagac tttgaaagcg ccgcagcggc tttcacaaca tgccgacggg
     1381 ccggcattgc cccgactttt gtggacatga cgctttgtgc gcttgcgatt cgccttcaag
     1441 ccccgatatt gacactggac aaggacttcg ccaggtacgc cacggtgctg ccgattcaaa
     1501 ttcacgactg catgaccagg atgccgtaag gcgctaggcc acgaccaggc cggaatcatc
     1561 gaatcgcgtg taaatgggcg tgtatataag gcggccttcc tggcgctttg cagaagcgag
     1621 gggcgaaaag accagcgccg ccggcgaatt tggatgcgaa tcaaggaaca ggttcgcgct
     1681 gcgcatccga ccggcggcgc cgctcttgac ttcgacagga tagacttttc cgccgcgcga
     1741 cattatgaaa tcaacctcgg catttccgcg ctgcccagcc ctggaccagt aatacatcga
     1801 cccattttca gacccgccag acgcaagcag ttcctgaccc acaaactgct cgacgacggc
     1861 gccgcggtgg acggcggaaa gatcatttcc cgaaaacagg acgctggctg aaaccccaag
     1921 caggtactga agaagtccca cgtccaggaa cagtggcttg aaacgtttgt cactggcgcc
     1981 ggcgccaagt ggaagtgcgt ccccggcggt atcgcgaacg ggcgtaacca gcagcgcccg
     2041 cgccagaatc tgtaccgacg cctttgtaac ctcggtcctt ctggctggat cgatgtgtga
     2101 atagatcacc tggccgccaa cggcgcccgg cagactcgaa aaaactgtct caagactctc
     2161 cctgtctgcg cgccgctggt aacgcgccag ggatcccgca agcgatgacg caagatcgga
     2221 gtgaacccgc gccgcggccg caagcgaatt ttcagcaatg aaccccgaca ccgcctcggg
     2281 catgccacca acaagcaggt atgtccgaag ttcgcccagc agcttcatgt gaacagcatc
     2341 cgaagccgtg tgtggcgatc caagtgtcgg aagggccgct gaaagcatgt cgagcccgcg
     2401 cgcgacaagg aattcgtgaa aggtcattgg gtgcatgcgc aggaaactga cgcgccccac
     2461 cgggaaggaa acttccccca acgcgaactc aagggccgac ccggcggcga tgaccggaat
     2521 gtcggggcgc tcttcataca gatacctgag tgcagtcaat gctcgtggct gggcctgtat
     2581 ctcgtcgatg aaaaggagtg ttcggccccc ggacaaggga ttaccgcaaa atgcctcaag
     2641 ctgcgggatc agggcagagg cacccaggtc accatcaaat atcgcccggg ccgaagcatc
     2701 gcgttcaagg tcgatcactg aggtattgtc atagtatgcg cgaccaaacg ccttgatggt
     2761 ccacgttttt ccgacctgcc tggctccgcg gacgatcagt ggttttcgag tcggcgattc
     2821 cttccatctt gtaagctcga tttctgcaga tctccacacg gcgggcctcg atgtaaaata
     2881 acaatccaat tttcacaaca agaacgtaaa atatcaatcc atattttaca ttggagacgt
     2941 aaattttcga ggttgtctgc cgcccccgcc ctgctatcat gggtcatccg ccatcgtcgg
     3001 aggccacatg gaaaccgtta ttgccatgac catttcgtcg ttccccgacc gcgagcgcgc
     3061 ccttgaatgc gcccgcgccc tgatcgagcg caaggtagcg gcctgcgtcc aggtgacgtc
     3121 cccgattacc agcgtctata catgggattc aaagacctgc gaggacgacg aagtcctgtt
     3181 gtttgtcaag acgacgctgg acagaaccga agaagccatc cggcagatca agacgatgca
     3241 cccctatgac gtccccgaaa tcatcacgct gcccgtgatc gacggcttcg gcggttattt
     3301 cgactgggtc cgcgacagcg tgaaatagat actccccgcg atgaacgatt tgtctggcag
     3361 gcttccgtat gctagcttcg cctttgcctg gcaacctgga gtatcccaga tggttcatcg
     3421 ttcgcttgca aacattttca tcatcgcggc attgttcgct ttcggatgtg gcggcggccc
     3481 gactccgcag tccgacgttg ttcaggacac gaccgaggcg gataccgttt cggacacggc
     3541 gctgccggac attgaccagg ctgacgtccc ttcggacgat tcaaattcgg acattccctc
     3601 ggacattgca ctgcccgacg gcctggaaag gccggttgcc gacccggtcg cggccgcccg
     3661 ggcgttcagg ctgtactacg ccgaacgcgt cgatcgcgcg gtggtggctt tcaaccggtt
     3721 catgctgttt ggcgacacca tgttcggcat caacatccgg aaggtgggcg tggcgcgcag
     3781 cggcgacacg ttcgaagtcg tcccgggacc aaaggacaac aaccagatcg gcacgtcggt
     3841 ccgggccgcg tggaatgcgt acaggctgta caggacacgc acccttgagc tggcattgat
     3901 tcgcatgttt aacggcatgg aattcatcgg cacggtcagc ggccacgacg gagtgaccgg
     3961 acgcaacgcc tatccgaact ggaccatgac catcaacggg cctgccggaa ctgtcggccg
     4021 cacgcgggac ggcaccacgg tcgaatcacc gtacgtttct gacgccggac tggaatccga
     4081 aatgatttcg acgttctttg gcgacatgga cgtgacctac aggctggaac cggccgacat
     4141 cctcttcaac tacatgccgg ccgttgaaac cggttcctat gccgtgacat attccttcag
     4201 catgctgcct gacttcctgc gcatcagcga ctgctgcact tcgatgatga aagtgccgga
     4261 tgactatccg tgggcgggcg cgttctggtc gaaccacaat tcgcgagaca acttcccgga
     4321 cctggtattc ggatacctgg ccgcgggtga ggcgatggac gatccggacg ccggtcccga
     4381 actgaaggcc gccgccaccg ccgcctggga agccggccgg aaggttggcg acctggtcca
     4441 gcagaacgac agccggataa tgacggtttc cgaacacacc acgtacgacc agttgactgt
     4501 cagcggcgag atgcggcccg acggcacggt cgaggccgaa accctgggcg cgatgtcgga
     4561 ctgccagatg aactacgccg cccgcgcgct ttcgacggat ggacttgacg cccaactgcc
     4621 ggagctgccc ctgcccggag cgctggaaaa cctgattgcg ccgttcgtcg atgtcgaatc
     4681 cgggtgcgaa tggatggaag gcggccacac atgcacgcgg ctggccgaat cgttctgcgg
     4741 caagacctgg ggccagctta acgaactgac gatgggcggc aagggcctga tcgacctggc
     4801 gatggaactg gacgaagagg atcccggcgc ggccggcgcg atcctgggcc agttctatgg
     4861 aaactacgac cagccgatga acgccgccct ggccgtcgtt gaatacgccc ggttaacagg
     4921 caagcaggat ctgctggaac tggcgcaggc gacgcttgcg gaaatgaccg acctgtcacg
     4981 cacgttcgca gacgtcatat tcggaagggc ggaccccgcc gagcaggccc agatgcgata
     5041 cgaaacggcg ctgatcgacg gcctgggggg actgaccccg ccggcgacgg acttcctgga
     5101 cttcgcggtg gccgaacagc agatggcggc catggaaggc ctgctggaaa tgccggactc
     5161 gaccgtcgcg gcactgctga ccgagcagga aatcgccgac cagatcgacg acgaacagtc
     5221 cgacgccagc gctcccgtga aaaagcgata cagggacaac tggggggaca ttcccccggt
     5281 tcgtgtcgag ggtgaaggct atcaggcgaa ggtgttcgag cgcgacacgg catcggactg
     5341 gcgcccggtt gaacgccccc atcacaggag gatgggcgga atcgacctgc ttgaagccct
     5401 gcccctttgc gtgaccaatc cgcaggtgct tgactgcacc tgggcaaagg ccggctgcgc
     5461 tcgcccggac ctgtaccatg atgggaaagt tgacgatgcg gatcgcacca tctttcaaac
     5521 gaccgcacaa gggaccaccg gctgcctgcc cgcgaacaac tactgcgacg gcgcggacct
     5581 ggaccacacc ggcaccgtcg atgcactcga caccgccttc atggacgccg cccagggctg
     5641 ccattatttt ctttcgtcgc aaccttctga caacgcaaga taatctcagc accctcatca
     5701 aagataaaaa cttttgcaca aagtgagcga agtagatggc ctggacatga agattccacg
     5761 gacggcagtc ggaactggaa cagaatcagt gattcccgta cagctcgccg gggcggacct
     5821 tgcccttgaa aacgcggtaa atgaagatgg tgtagccgat gactatcggg acgccgacca
     5881 gggcaatcat cagcatggtg gtcagggtgc gctgggtcga tgacgcgttg tatatcgtca
     5941 ggctgagctt cgggtcgttc agggccggga ccaggcgcgg gaacaggccc agggccgcca
     6001 ggccgatctg cgtcgcgatc gccgcggacg aagcgatgaa cgccagcatg tggcggccgg
     6061 acttcagcag gaccgggata agcacaacgc acgccatcat caaggggaaa agaatccaga
     6121 acaccggatt cttcaggatg ccgtcgaaca ggaacggcga cacgaacagc gacgcgaccg
     6181 tggcggcgaa gtacagaacc acccagccaa tccacaggcc gggtgcccat ctctgcatgc
     6241 gcgcctgcag gtcgccgtcg gtcttcagga ccaggtagat ggcgccatgg gccaggaaca
     6301 tcaccagcgc cagcacgccg accaggacgg cataagggtt cagcaggccc aggaaggtgc
     6361 cggcgaactc ggtcttttcg gtcaacggga tgccgcgaag gatgttgccg accgccacgc
     6421 cgaaaaggac gggcggaaga agactgccaa ggccgaaagc ccagtcccag gcgcgaatcc
     6481 agccgggcga atgcaccttg ttgcggaact cgaacgaaac ggcgcgccag atcaaggcga
     6541 ccagcagcag catcagcgcc aggtagaacc ccgaaaacac cgttgcatag acgaacggaa
     6601 acgccgcgaa cagagcgccg ccggcggtca gcagccagac ctcgttgccg tcccagaccg
     6661 gggcgatcga gtccatgtgg atgccgcgct cgcgctgatc tttcgcgaaa agcgacagcg
     6721 cccctacgcc aaggtcaaag ccgtcaagaa tggcgtaccc ggcgatgagc acgaagtaca
     6781 ggatgaacca gataacctga agtgcctgca aatccatcat gccacctccg gggccttctc
     6841 gggcccgtgc ttgacctcgc gcaccagcat gaagatgtaa aggccgccaa gcagcacgta
     6901 catcagcccg atcagggcga tgctgaacca cagttcgggc acgccgaccg tgattgaaac
     6961 gctgtcggca gtcttcagca accgatagac cgaccagggc tgacggccga cttcggcggt
     7021 gatccagccg acctggctgg ccaccagcgt caatggcgcg accagaagga caagccggtg
     7081 gtaccagcgg ctgatgtcca gcttctggcg ccagatcagc agcagcccca gggccatcgt
     7141 gccgatgaaa agcaggccaa gcattaccat ggtgtggaaa gagaagaacg tcaggccgac
     7201 aggcggcagt tcatccttcg gaaatgcatc catgcccttg acttcggcct ccgggtcacc
     7261 gaacgccagc aggctggcca gcttcggaat ctcgatggcg tagtccaggc gctgctcggc
     7321 ggcgttcgga atcccgaaag cgaccagcgg cgcgcccttc tgggtcttgt agatgccctc
     7381 gaaggccgcg aacttttcgg gctgggtctg tgcgacctgt ttcgcgtggc tgtcgccggt
     7441 cgggaaggcc gacatcgcgg caaagatgaa tccggcaatg accgcgatct tcagggcacg
     7501 gcgggaaact tcctcctggt tgtcacgcag cagacgcgcc gccgccacgc cgccaacgaa
     7561 gaacgcgccg gtgatcaggc aagcgaccag ggtgtggaag aaccggtacc acatcgaagg
     7621 attgaaaacc gcgtcggaaa agctggtcag gacggcccga ccatcgacaa ccaggaaacc
     7681 tgccggggtc tgctgccacg agttggcgac caggatccag aacgccgata tcgtcgcgcc
     7741 gaccgctacc atcaggatcg agaaccagtg aacgcccttg ccgactttgt tgcgaccgaa
     7801 cagataaagt ccaaggaagc tggattccag gaagaacgcg aacacgccct cggccgccag
     7861 cggcgcgccg aagatgtcac cgacgaagcg ggaatagctg gcccagttgg tgccgaactg
     7921 gaattccatg acgatgccgg tcgcgacgcc gaccgcgaat gtcagcgcga acagcttgcc
     7981 gaagaatttg cccatcctga cccaaacctg atccccggtg cgccacccgc gccactccat
     8041 gaagacaagc agccaggcaa gaccgatcga aattggcgga aagaggaaat gaaacccgat
     8101 cgtcatcgcg aactgcagac gcgcaagaat cagggcatcc attggttgcc tccgttaaaa
     8161 cctgttccgg gacttccggt ctgggtcaag cttgtccgaa tgtctttaca aaagtcaacg
     8221 cagaacgaag accgtcattt ggtgttctgc tgttccggcg ttgtctgcgt tggaatggcc
     8281 cgtgcggcac gcctcttgtt ccatgcgcgc cgaatcgccc aggccgcaat cgggaaaagt
     8341 acggccccga ttacaagccc ccatacctgg gtcttgataa gcagccttgc cgtgttatcg
     8401 accagttcga cgcactggca catcgcatcc ttcgcaccgg tactggcaag cagcgtgcgc
     8461 aaaacgaccg gcgcaaatgc cgtcgccacg gcaacgccgg ccagggcccc gaaaacgatc
     8521 cagttgaaca cccgacgcat gatcaaacct cccgggaata gaatcccgaa tcaatccggc
     8581 agtccggcac cggaaatccc accgactgcg accatcccgc catcagcgtt gccgactgat
     8641 tccagggaaa cattggcgct ccggtgaaga atggccggcg gcattatcct gtttcgcgca
     8701 aacgcatcca ttacgcgaag atgaacatcc gtctcaaaag ccttctcgtg cctgctgtca
     8761 tagacatatg gacgcgcccg aaccctgacc gccaccatgt tgtccagcaa cacctgcttc
     8821 acacgcatcg gcaccggcct gtccaggaac acgtatggac ttgtcaggca tgcctcctgg
     8881 acaagcgcca cggcctttcc gacatcctga tccgccccga tgaagaagtc catcggaacc
     8941 tgcatttcca ggctgccgta gttcccgctt gatgtaacgt cggtaagtat cttgttgttc
     9001 gggatggtga cgatgttgtg atccagcgta ttcatctgga cgctgcgaag tccgatcttg
     9061 atgatgtcgc cgtactcgcc ggcataagag acgcggtcac caacctggaa cggccgatca
     9121 accatgattg tgataccggc cagaaaggcc gcaaccagat cccgcatcgc gaagccgacg
     9181 gcgaaagcaa gggcgccgcc gatgacggtg actgctgtcg agtccagcct gaagctcaac
     9241 gccacacaga tgattgccgc cgcgatgtac aaaacgaacc gggcaaccga ctccagtttc
     9301 tggatggccg gacgtctgtt cgcaaaccgg attccaagcc ggttggcgat attctgaacc
     9361 aaacgcagca cgaccatcgt gccgacaatc accagaaccg acaaggcgac accgccccac
     9421 cgaacaaaat cggccagacg ggaaagatca ggcgcctcct gggccatggc cggtacagaa
     9481 gccagcatga cgccaatcaa aaccagtgca ccgcggggaa tccgtaatcg accgaagatc
     9541 atttcaaccc ccgaaacgat ggaaagatgc aagcagatga cgacgctgca ggaatcttgt
     9601 caccggccgg taccagttcc atgacacccg ataacgatcc ccgacaggtt caaaccatcc
     9661 cctggcggtg ccatatcgaa gaagatcaag aacacggcct tgcgaccttg tcgatgcctt
     9721 cacaacatca tcgaccgctg ctggctcaag ctggataatg gcacgaacaa cgaacacatc
     9781 ctcgtcggac aatcgttcca gttcacgaac atcaggaacc tggaatggcc gaacaaacac
     9841 ctttccgtca agccccgccc caagctggcg gcgccagacg tcaagagcca cgccggggtt
     9901 cccgtccgca tagtcccaaa gcatcctgaa ataacccctg gcaaccctgg acacggcttc
     9961 ctcacgatcg atactgtcgc catcacgtat cgggcgatcg attacggcct cgaatgatgg
    10021 ttccatgtga acctgggcgc ttcttgccct caaaagcgcg atgatttcct cttcccgcca
    10081 cggttcgatc cggacgactt cgtcaaacac agggtacgcg cccctcgacc tttcaaagaa
    10141 ctgccagatc acctcgttga agaccagaat ccacgttgtt cgctcactgt gacgacgcgc
    10201 aatcccagcg aatttgtcaa agccatccaa tccacccatg accggaagca ccagacgatt
    10261 agcgtggtcg ataaggattg cggaatcatc gccccttgac tcggccgcaa ccgcgcagtc
    10321 ctggattgat gccgccggat ccaggcccaa tgcgacggca agagtcgccc gaagcccttc
    10381 gattccatcg acggggcatt caaccagcgt tgataaagga tggccatccc tgatgcgctg
    10441 cagaagccgg gacttccccg cgcctcgctc tccgacaatg gcaagcaaac cgccgcccct
    10501 taactcgatt tttttttacg atttccatga ccctggcata ggatgccccg gaaatcagag
    10561 tcccggaaga ccgatcaggt ccaagtcgat caaacacgtc tggatcaatc ggcatcaaca
    10621 cagtaccgga ttcctggtcg ccggccatcc gatccatgcc acgcctgaaa aaccatgcgt
    10681 gggcccgacg tgccaggtca aatcctgtga tccagccgcg aattatccag taagcacccg
    10741 aaataaaaag ataaatacca ccagaaaccg cggcagggaa actcttccat ccctttcgcc
    10801 gttgcgcgac ccaatcctgg aatgaagtcc gtcgccttga tgcctgaatc cggtcgaaaa
    10861 tgacgtcgcg ccaccatcga atgatcacca ggatcacggg gatgaccgag aaccagcaaa
    10921 cccagattac ccagcgataa atggtgccct tgcccacaag ttgcgagccg atggtcagaa
    10981 tcagtccaag gataacagtc gtgtaaccgg ccagtttcaa ggatctcaga cgtattgcat
    11041 cggtcgcggc ggtgccaccc gatccggatc tcgaaacggt cggccatgcg acaagtgcgt
    11101 cgattgcccc gataacgaag gcgccgcaca gcgtccagac gaacacggtc ttcaggatga
    11161 ccacttccag gcgggtcctg acgaccgggg gaagcaccca cacaagaacc agaatgaatg
    11221 cgagccactc gatcggtctt cgggcacgcg ccacgaacac aaccagtcga tggatcgcgc
    11281 tgacctgcgg aatccttgcg gccctgtccc gctgaacaat ccgttcaatc cacgcatcga
    11341 aaagttctgg actgcggcgg cgccaccacc agaacagcag aatcagtccc agcaacttaa
    11401 cggccgaagt gccggttgca agcagggaat cggaacgatc aacgcttccg tcagccagag
    11461 tgatgaacca tgccgcggtc gctgccaggt gataccgaag aaccagggtt acctggcgca
    11521 actctgccac cgcctgatcg aggccggccc tggtgaaacc ggttatctcg gcgcgacgct
    11581 tttcggaaag gatcgacaaa agcgccaggc ggtcggcatt cagcgccctg acttcctcgt
    11641 aaagctgggc ggccctcttc tgccggtatt cctgctcgtc cgatgcaagc tcgacggctg
    11701 tctggacaac cttcctgcgg gcttgctcga gcgtgctggt atcgacgccc ggcggaacat
    11761 cctgaatgat ggaagagcca attgacggga cggcgctgtc tgcggtcagt tcgcgcaacg
    11821 cgtcggccag ttcgtcccgg gatgagcgca gaaaggaacg aagatcatca taaagcgcgt
    11881 cggccgactt cgcgtccggg gccttcagtt cccgcagttc gcgaacaaga cgcctgaaac
    11941 ccggcacctg ttccaggcgc agatccagaa tttccagccg accggcgagt tcctttcgaa
    12001 atttcgcctg gcgtgccgaa atgatctcaa gttcatcgaa ttcagccgat acggccctct
    12061 gtgcttccac gcgggccagt gcggccgcct cggcggcctt ctgccgcagg acctcgaccg
    12121 cctgggcttc cgggtcgggg cgtacctgga tcatttcatc aacggcggcc gacgcgcgat
    12181 cgatgacatc ccgcatatcc ctgccggtta cgccggcgga atcggactcg accgccacac
    12241 ctgggtcgcc gcccggaagc gcatcgcccg ctgcttcatt cgcggcgtcg ggagcccgga
    12301 catccctgga tgaagcgtca tcatcggacg aatccagatc cgatcgggtt tgaatcacat
    12361 cggccgaccg acccgaagca tcatccgcga gcacaggcgg ggcagttaaa acgatgccaa
    12421 tgcaaaccag gcacgcaacc aggcgctgtc ccgcaatgaa gccgtggatg gatgcgccgt
    12481 gcctgcccgt catcagtagc tcggtaagca ttatcggccc catgtacacc ctgattatca
    12541 cggatgccca agtctgaccc acctcgcgcg cgtagtaaac agaatttaca tacttcgcgg
    12601 atttgacgca tgaacgtgct gcggtctcaa cctttgaaca caccggaatc ccgcagttat
    12661 gattggacgt ccgatccggc gacctggacg gcgctataat ccgacgaata tggggcggtt
    12721 cgacgacgcg ttgtcgtcgt attccgaatc caatgacctg gagacaacgg tgacaaagac
    12781 cgaaaccgaa tccgaacgac gcgaacgacg acttgccgcc gagcacgccg tctggcgaat
    12841 cctggcttcg gccgaccgtc tcgaaggcgt tgtaccatcg attctgaagg aagtatgccg
    12901 cgccggggcc tgggcgcttg gtgaactgtg gaccccaggg gacgacggac agatcctgca
    12961 ccttaccgac gtctggaacg atacgctttt cgacctggat agatttgtcg aagtgtcgtg
    13021 ggtcacgaca tttcagtcgg ggatcggctt gccgggacgc gtgctcgaaa ccggcgcccc
    13081 gacctggatt ccggacgtgg ccgccgaccc caacttcccc cgactgaaac tcgcagatga
    13141 agccggcctt cacgcggcct ttgggttccc gatcaggata cacggccagg tcatcggcgt
    13201 aatgacgttt tacagccccg actttcgaca gcctgatccc gaactccttg aaagccttga
    13261 tgtgatcggg ggccaaatcg gccatttcat tgaaagggcc agaaccttcc aggcgcttgc
    13321 cgaaagcgag gccaggtggc agcgcatcct ttcaggcgct ccgttcggga ttttccactc
    13381 gacgcttgac ggcaagctgc tggacgtgaa cccggccttc gccacgatgc ttggctttga
    13441 atcgccggag gaagcaatat ccgccgtgaa ccgaacctcg attccccagg ctctgtacgt
    13501 ggttcaggca gagcgggacg atttcctgcg ccggctgcgt gaacagccgg gaagctgggt
    13561 ctctgccgag gtccagggcc gccgcaccga tggaacgacg gtaatcggtg aactccatgt
    13621 caaatggctc tatgaatact tcggccacag cccggcaatg gaaggcttcg tcaccgacat
    13681 cacggaacgc aagcggatgg agcgggaact gcgatcagca ctggatcgcg tgcgggaact
    13741 gaccttgcgg gatcacctga ccggccttca caacaggcgc catctggaag accgcctggc
    13801 aatcgaaatc gcggcggccc gcagaacggg cgacgacatc tcggtcatca tgttcgatat
    13861 cgatttcttc aagaaaatca acgatacgca cggccaccag atcggggacg tcgcgcttca
    13921 ggccctggcc gtcacgcttg ccgccgacgt caggccggag gatgttcttg caaggtatgg
    13981 cggcgaggaa ttcgtgctcc ttctgcggca gacaggcccc cgggaagcct gggaggccgc
    14041 ggaacgattg cgacggatgg tcatgacacg accgatcgcc gtgcctttcg gtgaaatcag
    14101 gatgaccata agctgcggcg tggcatccat gtcggaatgc ggtgcccggg atcaggacag
    14161 cctcatgcat gcggccgatc agcgccttta ccaggccaag gctcagggac ggaacagaac
    14221 cagcggcgcc ggggtgcttg aagacattcc ggacttcgtt ctgtagtccg ttccatgcgt
    14281 ggtttgtgcc atgagccgtc cacgccactc ttctgcgtgc ggatccccgc gaatcaggac
    14341 ttccactcaa gcagtcggcg ctcgccctgc aaggcacgga taccggtaac gtcctggctg
    14401 acctccaggc atccgcggta ggtgccggcc ttgtcacgaa gcgcgaaata acggatgtgg
    14461 atgaatctgg gcccaagctg cagccagaac tcggccacgc tctgccggcc cgcgcggaac
    14521 gaatcgagaa tgtcctgaac cacgttcagg ctcttcggcg gatggcaaaa ctgaaccttg
    14581 cggccgataa cggccggact acgcgggaag atccggtcgg ggccctcgct gtaaaacctg
    14641 acggtgtcgg cttcgtccac gaaggacacg tccaccggga ggttgcacag gatcaggtta
    14701 agctgttcca tgtcgatgtc gccggtcatc aggcccgcaa gccctgacaa tcgaacctgg
    14761 gcgggagccg caacgtgtcg cttcgcgggg aaaggcgcgg cgggagtcgc aaggacgtaa
    14821 ccgagttcgt cctcgccgtg ccggacttcc gcccattcat cgtcggtgaa cgtctgcatg
    14881 gccatcggga aaagaatctt ttcttccttg tagatcatct cgacgatgtc gcgggccagg
    14941 gccggtgcgt cggccgcgaa tgtagcggca tccccggcga cggcggccgc gcgaaccttt
    15001 ttcatggcgg cccgaatctg gtcatggacg ccccacatga cctgtgacgg ccccatcacc
    15061 ttgtatcgtt caagcagtgg gaaaagctga ttttccttgc gctggtagtg gttttccagt
    15121 ccggccagcc cgtccaggat cccgattgcc gaagaaagcg cggctgcggc attccctgat
    15181 cccgaaacct gacgggcaat ctcgccaagc cgattcgcaa gggtcgaaat taccttgttg
    15241 gcggccatgt atgtatccac gggatggccg ggaggggcct gtagttcggc atgaccgtcc
    15301 agtgcctctc ggaaggcgcc cacgtgcaca tcgcacagac gctgcacctc ggaaacggga
    15361 agtccaccac gaatcaattc ttcctccatg gcggcgatct cggtggcctc cacatcctcg
    15421 acagcggcgg cgaacttctg gcgcgcggct tccaggtcgc cacccgcgtg gaggtcggaa
    15481 ataacctcct tcagcgcggc cagcctggat tcgcgtgtct gggcgggggc cgtcgactcg
    15541 cctatttcag ggaacgcgcc ggtgtggcgg gctatctcgg cggcgatggc ggcgaccagg
    15601 gcgtcaagat cgatatttgc aatcccggcg gcggtgcgca cggtcgcaat gcgaccgatg
    15661 gtggcacggg tcatcttgtt gcgcagcatt tcaaacttcg actcgtagga tgccaggaaa
    15721 tcctcaagga acggataggc gttcaacagg tcgttaatct tcgtgtcggc tgaaaacttc
    15781 atctgcatac tccgcataag ccgcgtccat tatcagataa cttttgtccg agttcaagag
    15841 ggcgcagcga ttaaactgcc gaatcgggat gtttgcgggc aattgatgac ataatatgtc
    15901 cgaatatgca acactccgtg atgacgtcat tttgcgacgt gtgagcaact gttttggaga
    15961 gtttatatga aaaaggccct cgaacccggc atgccggcag caagcgcgat cgaaatcggt
    16021 gccctggttg aatatcagca ggattccatc gtcagcagga ctctggtcaa gcacaacggc
    16081 ggcacactga ccctgttcgc cttcgatgaa gggcaggcac tttcggaaca caccgcaccg
    16141 ttcaacgcca tcgtccaggt gcttgatgga acggttgaac tggtgatcgg cggcaagccg
    16201 gttccagcgg tggcgggcca gaccgtcctg atgccggcga acatcccgca cgctgtcaat
    16261 gccatcaagc cgttcaagat gcttttgact atgatccggg atgtgcagac gactgcctga
    16321 aaccagacgg ggcgactacc ttaatgcagg cgaccagggg attgcgggaa tccagaacgg
    16381 ttcttgccat gattcgaatc ttctgccgcg gttgccacgg aaccgacggt cgcaacctgt
    16441 gcgggaattg cgacgagctt ttccgttatg tccaggctcg catcgaaaag tgcaggtatg
    16501 acgacgacaa gccgacatgc ctgaactgca gggtccactg cttcaaaccg gcaatgcgtg
    16561 aacagatacg gctggtcatg agatacgccg gtccccggat gccgttgcgt cacccgtatc
    16621 ttacgctgat gcacatcctg gacggtcacc ggaagccgcc gccttgaacg aatcgacatt
    16681 tcgggtataa tccccggccg caagccgggt aacttccccg acccggtcga ggttcctgat
    16741 gaacaagact ttcctgaggg acaatctgaa gcgcctcgtc ctggttcttc tgggttcctt
    16801 cctggcggcc cttggcgttc agcttttcct ggttccgata cacctgctta cagccggcat
    16861 cgccggcttc gcgctgcttc tttcttacgt cactccgatt ccggccggcc ttggactgtt
    16921 catccttaac gtcccgatct tccttttctg catgaaatac ctggagaagg agtttcttgt
    16981 ctggagcatc gtcggcatga tcacgctttc cgcgtcattc gcgctgacgg cgccgcttgc
    17041 ggatcttcat ccggtcaagg acatgtacct gaacctgatt gccggcgccg tggtgtccgg
    17101 gttcggctgc gggctggtat tcagggcccg ggcgtcccag ggcggcaccg acgtcatcgc
    17161 ggccgcggtc cgcaaggtca gctcggtcag gattggatac cttctgttcg ttctcaacgc
    17221 gtcggtggtc gtgatcctgg cgatcatgta cgggcttgaa cctgctctgg caaccatcgt
    17281 cgtaatcgcc atcgagtccg tcgtcatcga acggacgatt atcggcgtgg acgggaacaa
    17341 agcgctgatg atcatcacca ggaaaccgga cgaaatcgcg gccgaactga tgaagcgagt
    17401 cgaccgtggc gtcacaatca ttgaggccag cggggcttac acccacgaaa ggcggccgat
    17461 cgtcctttgc attcttcgta cccgccagct tgcgattgcc acaaaaattg tccgcgacat
    17521 cgacccggaa tcattcacgt atgtccacga cgtcaccgag gttatcggcc acggcttcaa
    17581 ggcatcgccg atttagtgac gaacgcgact agagaatcct gaagagatag acaagacagt
    17641 agtacccacc gatcagaagg aacacggacg cagtcagtcg gcgcatccat atttcgatga
    17701 tggtcattgc gttgaatgcc tttgaaaggc ggttagctgc gaaggccagg atcacggaaa
    17761 agacgattac gggcagcgcc gtaccgattc catagacggc cggcatcatc acggacgact
    17821 catgcttgac ggccaggggc accagactgc caaaaaagat tgctgccgaa agcggacaga
    17881 acgtcaacgc gaacaccagc ccgagaaggc cggcgcccca gattccaagc ttatcaacct
    17941 ttgcttgaag tctgttgaag aagtttgacc cgccgccggc ccacggccac ggtatgaaat
    18001 caagcagcag gatgccgacc agccggccgt ccttcttcac cagtttgcgg tagacg
//
LOCUS       DAOPPA010000008        17355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_3916_length_17355_cov_3.846185, whole genome shotgun sequence.
ACCESSION   DAOPPA010000008 DAOPPA010000000
VERSION     DAOPPA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 17355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17355
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3916_length_17355_cov_3.846185"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1936)
                     /locus_tag="PKH54_00605"
     CDS             complement(2..1936)
                     /locus_tag="PKH54_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98414.1"
                     /translation="MPGRLNMVVAMLALLVGLALRFMVAAGDFPSGDPRPFSDDPPYH
                     VLRLQGLADGTSPFPGSPEARDPMVAWPTGDTPIWPWGFDILLAVTTPLQQACGTSCM
                     INAAGMMIPLLAAVAGLLLFLLARTVAGGSAPAFAIMAFMMIPAGVAYTHAGRIDHHV
                     LEPLLPVLALLFLFRGGSGRLTGAACAAAGFACGIQAAFFPASPALTFPLFVMAGLYV
                     QGPARVSILSAGLIAGTLLSLALSPYPSSWTFHSPSLWHMAMALCLSAGLIVFKSVLA
                     ALTAATAASPSRSARIIPPAVSALAATAAFGALILLSDVTFPEFIRATIAGFNYTTAS
                     PFAALSLEANSFFDDPVRTMQVTGWLLVPASAGLVALGFDGIRNRNRRSLTIVATATT
                     FITLGLVQRRFMLAATPFIAILAAQGVTMLSGRIRMLAGNRLLLARTMPFLIAAITLT
                     PSLLGDLRLTSLTPLDRAAYSAAQAVKRHREAGSGVVPAGALTPWAYGHLFKMAAGTP
                     TVCDNFFGVPGADRALTRCLGLNYSTDDRDIERQLGELKVTYVVLMPPSPEQVVAETR
                     AIGLNPEEWVDHEGRLSTKFARSFLGRTGMWANGARPGQKGPFGLTLLGRFRQIDPST
                     SSIAAEVLVLTRNPDSRFQP"
     gene            2238..2822
                     /locus_tag="PKH54_00610"
     CDS             2238..2822
                     /locus_tag="PKH54_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC98415.1"
                     /translation="MLNKFRKFNFKGFTLVELMVVVAILGILAVIAVPAFIKYMRRAK
                     TTEAIDELDKIYKGASYYYTAPHVDATGKKIYCQFPADQGTTPAASCCEAFSGPDTDK
                     DDRCDSNSLAWNEKTWSALTFQVTDQVYFVYSWDQNDEVMSVAKATANAHADLDCDGT
                     QSTFQRIINGDPKATYAECASVGASAFFVDNETE"
     gene            3010..4689
                     /locus_tag="PKH54_00615"
     CDS             3010..4689
                     /locus_tag="PKH54_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOC98416.1"
                     /translation="MKNTLSVLIALSLLFHAAQAGEGRVPGSGEDFTRKDRTELLFQT
                     KFQFTPENVPVVTVGIVEGVGDVAFSSEGPLVFQPEGEGGPTVRVGRSRIDCIATIED
                     SRPAVIRYWAALERVPAMSLATVREARKRWTDKGLNIRTFEQGSVFGFYGRVLDNRRV
                     VIVEDVPFADRAAAMKRLDELTSSHGLSTPEPYEEVLSRPDGRVRVRCTNVDAAMAFP
                     GIVTVSPSAGKTIRVRNVEFHKLYGQGKSEDRSYRGNLILTADRSGRLAVVNQIDAET
                     LLKGLVPSEIFTESPMEALKAQAVVARGELFAKLGNRHTADPYMVCADVHCQSYRGLE
                     REHPRASRAVEETAGMMAFHEGALVDSVYSSSCGGHSEAGANVWQGSNHPYLGGTPDL
                     PDKTRTRLFKDGVTEKAVRDYIDNPPKDAFCGRSRFGKSTFRWSSTLSFSAISEGVAK
                     ETGVSIGQFTEFEVLHRGVSGRITRLRVKGSSGSVDIAPELRIRRALGSLKSSAFYFD
                     VGNGSVTFRGAGFGHGVGMCQNGAIGMADAGIRYDEILKHYFSGSEIVKIY"
     gene            complement(4683..6308)
                     /locus_tag="PKH54_00620"
     CDS             complement(4683..6308)
                     /locus_tag="PKH54_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98417.1"
                     /translation="MLKRFGYLNGVLVVVASAAVVLSTMIPYHMIDERRPDGTVFYGI
                     STNLPDAASYMMWFNQHAQGHILVENRMTVEPHPRLLPNPAWMLIGLYKAVFGSNTLF
                     SYNFMRMMLSLAYLPLLFLVLSKMLPGRRWTWMAWWIIAIGGGLGWLETVGLRVPSAD
                     WMTELWSWPSMLHYPHFVLSLILVLLFFLVSMKANRFFIKSLYVLSGVLMALLFMVHP
                     YTAGTILAAIVIMRVVIVWLKKRNTIGTVQLDPAFWWVIGISAPAVIALLLQNHFNPV
                     AAAWASQNHMPSPPIWEYVFGFGLPGVAAGLLVARMVKTRVPESPEIMFGMVWLGTAL
                     VLAYADPLVPFARRCMEGVHLIVVVMGVRYVAGIASKRIRVAAVVLMLATTLPAPLYH
                     INREVVPDNPGYVPEDMYEMLDAVRYWVKDGNVISDPRTSLFIAAETDAHVFIAHHEV
                     TPGFLDKIRAHYKYLTEPTTLKERLQLMRDVNCPWAVSTPELAYGMLKTENIDAEDLK
                     KVREFARGKSWVLLGLVDHQTSEGDGSEQQRSQ"
     gene            complement(6301..8349)
                     /gene="ligA"
                     /locus_tag="PKH54_00625"
     CDS             complement(6301..8349)
                     /gene="ligA"
                     /locus_tag="PKH54_00625"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOC98418.1"
                     /translation="MDSGKASDRIRWLQSELSRHARLYYEKDQPEIEDADYDALFREL
                     QDLEARFPEFSTPDSPTRRVGSAPARQFAQVVHNVPMLSLQNAMDDSEFLAFDERVRK
                     ALGLQSVDYVVEPKIDGLSCEIVYRDGVLVQASTRGDGQVGEDITANVRTIKDVPQRL
                     KALSFNAEPGLFDEVSPPLPELFEARGEIYMPTEPFRQMNQERLDAGMSAFANPRNAA
                     AGSVRQLDPAVTASRPLRFFAYTVGRLDDREILSQRDLLRRLREYGFQVSSLIRPARG
                     AAEVLDAFKRLGSERDSLPFEIDGAVVKVDSFELQRELGEISRSPRWAIALKFPPRRE
                     KTVVNRIVVQVGRTGVLTPVAELEPVKVGGVMVRRATLHNIQEIRRKDIRIGDVVEVQ
                     RAGDVIPEVVRPVISERDGNETIFNMPDRCPSCLEPVAGRQDEIAWRCTNISCPAQFR
                     EHVIHFASKRAMDIEGLGERMADLMIEAGLIHEISDIYALTVTQISALDRMGDKSAAK
                     LVAAIEASKRRPLAALINALGISLVGETTARDLASNFGSLAALAAASTEDLRQVQDVG
                     PIVAASIFDFFQSRRNLDLIERLRQAGLTMMNESAAGGGPFEGKSFVFTGSLQKMTRD
                     KASDLVISLGGKVSGSVSRGTSFVVAGSDAGSKLAKARELGVAVISELDFLAMAGADA
                     "
     gene            complement(8467..9879)
                     /locus_tag="PKH54_00630"
     CDS             complement(8467..9879)
                     /locus_tag="PKH54_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC98419.1"
                     /translation="MISRILAASMLVATLMVAGCGDGSSQTDEGGTPDVVTPDNGNDV
                     VPDVANPDEGTPADAADDTPLVDAQPDSITPPCPPHKCIDDGNGNMIENPNPGCVGEL
                     CVAGPEAAPDPMSFGPFPVGIRQFVYYDPNPDNNNEDGSPRKLKIDVWFPTTEEFRGG
                     DPFIYDVKEECGPDVLEKYEGVEIGRFPVDAVLDAPVRHGDGRFPVVIFSHGAFGIRF
                     QSIFFTIQLASHGYIVISADHQFNTLNEILVDGYEASELLYSAQHRPKDIFALLDWIE
                     AKGEDTADEFYDLVDMENVACTGHSFGGLTSYIVTVDPRIDCIIPMSPAADMVNAFTI
                     YFNNTPIEELSIPTLMMAGEQDKTLQYQPQQRDPFDTQPAPKWLLSLPRAGHYTFTDS
                     CMMNLEELQAYWEDADDAIRDGCGPDNFDWREAHKVINLYGISFLNHFLRHSPAAGAV
                     LVPESAQQWGEEVEFIPYTE"
     gene            complement(10037..11170)
                     /locus_tag="PKH54_00635"
     CDS             complement(10037..11170)
                     /locus_tag="PKH54_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOC98420.1"
                     /translation="MPLFADTDSLVDSLARSLLDEPCVAFDTESDSMYHFREKVCLIQ
                     IASDATGTILVDPLSRVDPGPLGAVMASPSVTKIFHGADFDITSMKRDFGWSFSNVFD
                     TALAARFLGWPSVGLAAVILREFGHEIPKGPQTADWSVRPLPANMLEYAAGDVNYLVE
                     IYRRMVRYLQEAGRLRWVIEESAAIADIHAASPLPAPADFLKATGACDLDGSDLAILK
                     RLFAAREAVALKLDRPRFKVIGDDALVRMAISRPRSPAALERIRAVPRPVASHPEPWL
                     AAIRDGLSDPPVEIPRLRRRPPFDPRVAESIDRLKKWRAGASGRLGLDPGLLLPQRLI
                     VAIAAAFPASIAELGQVPGVHAWRCDEFGAELLECISHSGHAG"
     gene            complement(11197..12621)
                     /locus_tag="PKH54_00640"
     CDS             complement(11197..12621)
                     /locus_tag="PKH54_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98421.1"
                     /translation="MKTLLKTLAAVAFFAVAVCAAPVSAQEASDEAVDELVPEAEPAA
                     DENPEPSYVEVEPAADEPVDTVSATADVKPKKKVHPYKAFFDKELEALGKDDRFGVTA
                     QLPKRYLSVKWDHSMMTVDSRYNDKHELGPAIAPIAMPGGYLDLGLNGAGAGHVFQAS
                     YGILGNFDWYFELPFQSMHVRIDPKFLDSSKQPVKNPNDPNDKTYASRMGIAKLYQFI
                     PQLGRPVPALRYDADWVLADINTGFSWNPWRTKHLSTSLTVRAFLPTGRVASANANLT
                     MATGPEIDVGTGSWGLGFTNGWDLRVFKYKHWVDIVLSSEFSMAYFFEQKRDYPTNFT
                     TEPNRSILQAFNDPNVFAQFPDLRHLKGQYSYTPGFGVSWMGQLSVQLAILGLNFGYG
                     VQHSQEPEIQGDWYFYQMVRSLQLLGQNTIQAIQVGASLSLIPLYLPVQVDFSWRKIV
                     DGYNALAMEDYYNVIVKVFIPMKW"
     gene            complement(12636..13295)
                     /locus_tag="PKH54_00645"
     CDS             complement(12636..13295)
                     /locus_tag="PKH54_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98422.1"
                     /translation="MRRTASILVLLVSAAILTPGCYTYESQTADLGFMADGWGDQGTE
                     ADVPEDTGNVTPDVVQDVAEDVPECTCTCEPQPCTCTCEPGGECTFDPNGFPETGRVG
                     GDPCTTDTQCVTQLCATTDLLNAVWPGATAPDGMCTMIGCSGNTDCGTGAICLDPTAL
                     DPTVPFLCGAPCESDIDCRCGVDYTCLDSQKTDGDGNPIKACLPRSLKNLLECGAAVC
                     P"
     gene            13499..14512
                     /gene="pta"
                     /locus_tag="PKH54_00650"
     CDS             13499..14512
                     /gene="pta"
                     /locus_tag="PKH54_00650"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOC98423.1"
                     /translation="MLQFELMNSFKTRARKNPKTIVLPEGSEPRTLKAAAIINEEKLA
                     TVILVGQESGIRAAAAAEHANIRDIRIVDPDASELLPGYVQTFYELRKAKGMSMVEAD
                     RMMRDPVYFATMMVYAGDADGLVSGAIHSTGDTIRPALQIIKTAPGITTVSSAFIMVV
                     PDCQFGDEGLFIFADCAVTPNPDAEQLAGIAVSSAETARVLCDMEPHVAMLSFSTKGS
                     AKHPLVDKVVEATRIARELAPELDLDGELQLDAAIVPKVGALKAPGSPVAGKANVLVF
                     PDLQSGNIGYKLVERLAKASAIGPFLQGLRKPVNDLSRGCSVEDIVNVVTITAVQAGG
                     VGR"
     gene            14580..15926
                     /locus_tag="PKH54_00655"
     CDS             14580..15926
                     /locus_tag="PKH54_00655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOC98424.1"
                     /translation="MIILSLNSGSSSLKFQLFDYDESITICSGLVERVGIAGSFIKFS
                     NYGGESVKIEHPCPDHREAMKLLLSTITGSEHGVIDSLDRIAAVGHRIVHGGEKFTKS
                     VLITDEVVDVVKSLYDLAPLHNPPNILGVEAAREVMPGVPHVGVLDTAWHQTMPEHAY
                     LYALPRNWYTKYGVRRYGFHGTSLLYCAKRAAALLGKDPLKTSIVTLHIGNGASANAV
                     RNGVSFDTSMGLTPLEGLVMGTRAGDHDAAIDLMIMEKEGFTPAQMNSFLNKKSGVFG
                     ITDEKYVDRRDEEDAAEAGDKLAQLALDIEVHRLKKYVGGYIAALGGVDAIVFTAGVG
                     EMSPYIREHVCRGLECMGIEMDFVKNAPCKTRSAECDLATPESKVRIFVIPTDEERVI
                     IEDTVAIMNGTYDDHTRFVYPFQLPGYVNKEREAALARDMEKKSWLKDCVVRPPKA"
     gene            complement(15987..16736)
                     /gene="ccsA"
                     /locus_tag="PKH54_00660"
     CDS             complement(15987..16736)
                     /gene="ccsA"
                     /locus_tag="PKH54_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOC98425.1"
                     /translation="MATAALLAGDIFRNRLVRFSGIAALFGGTVATVVAIILRWLATG
                     HPPLSNLHESFVFIGFATGLAALVVELTFRTRLVGAASALVAVLALGYASVLDSSIRP
                     LMPALKSNWLIIHVTSYFLGYGALAVATVASALQLAWSFVPGADPASLKRIDLLAFRL
                     VLLAFPLLTLGLTTGAVWASFAWGRYWGWDPKETWSLVTWLVYALYLHLRAMRGWRGR
                     RMAVVALVGFVFVLFTFLGVNFFIGGLHSYR"
     gene            complement(16760..>17355)
                     /locus_tag="PKH54_00665"
     CDS             complement(16760..>17355)
                     /locus_tag="PKH54_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOC98426.1"
                     /translation="FRIDWDRSAPHLLHVGVMDAGVRTSIKVEPGSTYDVPGTDYRIE
                     VSRFVADLLVDREQGIISRSDNPANPAMLLLIHHAGETEKRWVFSAHPEFSMSKDGNI
                     DIIYRHDAKPLDFASRVTILDKDGSTTSAIIKVNQPKKHRGWSIYQTSFDVARPGWTG
                     LEVVYDPGVAIVFLGIALMNLGVFLVLHRRFSKSATR"
BASE COUNT         3662 a         4998 c         5330 g         3365 t
ORIGIN      
        1 ttcaaggttg aaatcgggag tccgggttcc ttgtcagaac aagcacctcg gcagcgattg
       61 aagacgtgga agggtcaatc tggcggaatc gtccaagcag cgtaagtccg aaaggtccct
      121 tctgtcccgg ccgggctccg ttcgcccaca ttcctgtgcg gccgaggaaa gagcgggcga
      181 acttcgtcga cagtcgacct tcgtggtcaa cccactcttc cgggttgagt ccgattgccc
      241 ttgtctccgc cacgacctgt tcaggagaag gaggcatcag aacaacgtag gtaactttca
      301 actccccgag ttgcctttcg atgtcccggt catcggtcga gtagttgagc cccaggcatc
      361 tggtcagggc ccggtcggcg cccggcacgc cgaagaaatt gtcgcagaca gtcggggtcc
      421 cggcggccat cttgaacaaa tgaccgtatg cccatggtgt caacgccccg gccggaacaa
      481 cgccggaacc ggcttcccgg tgtcgcttca cggcctgggc tgccgaatac gccgccctgt
      541 cgagtggcgt aagggaagtc aagcgcaggt cgccgagcag ggagggcgtc agggtaatgg
      601 cggcgataag gaagggcatg gttcgggcaa gcagaaggcg gtttccggca agcatcctga
      661 tcctgccgga aagcatcgtg acgccctgcg ccgcaagaat ggcgatgaag ggtgtcgcgg
      721 caagcatgaa gcgccgctgg acaaggccga gcgtgatgaa ggtcgtcgcg gtggcgacaa
      781 ttgtcagcga gcgccggttc cggttcctga tgccgtcaaa accgagagcg acaaggccgg
      841 ccgacgcggg gacgagcagc cagccagtga cctgcattgt tcgcacggga tcgtcgaaaa
      901 acgaatttgc ttcaagtgat agtgccgcaa acggtgaagc ggtcgtgtaa ttgaagcctg
      961 caatcgtcgc gcggataaat tcggggaaag tcacgtctga aaggagaatc agcgcaccga
     1021 atgcagccgt cgccgcaagg gccgacacag cgggtggaat gattctggcc gacctgctcg
     1081 gggatgcggc cgtggcggcg gtcagcgccg caagaaccga cttgaataca atcaggccgg
     1141 cagaaagaca tagtgccatg gccatgtgcc agagcgatgg cgagtgaaag gtccaggatg
     1201 acggataagg tgacaatgcc aggctcagaa gggtacccgc aatcagtccc gcggaaagga
     1261 tcgataccct ggccggtccc tgtacataca gtccagccat cacgaacagc gggaaagtca
     1321 gtgccgggga tgcggggaag aaggccgcct ggattccgca ggcaaaaccg gccgcggcac
     1381 aggcagcccc cgtgaggcgg cccgacccgc cccggaaaag aaacagcagt gccagaaccg
     1441 ggagcagcgg ttcaagcaca tggtggtcga tgcggccggc atgcgtgtat gccacgccgg
     1501 ccggaatcat catgaaagcc attatcgcga atgccggggc cgagccgccg gccacggtgc
     1561 gcgcaagcag gaaaagaagc agcccggcga cggcggcaag cagcggaatc atcattccgg
     1621 ccgcgttgat catgcacgaa gtgccgcatg cctgctgcaa tggggtggtc accgcaagca
     1681 ggatgtcgaa accccagggc cagatcggtg tgtccccggt gggccatgcc accattggat
     1741 cgcgtgcctc cggcgagccc gggaatggcg atgtgccgtc cgccaggccc tgaagacgaa
     1801 gcacatggta tggtggatcg tcggaaaacg gtcgcgggtc gccagacggg aaatcaccgg
     1861 cggcgaccat gaagcgaagg gccagaccga caagcagcgc cagcatcgcg acgaccatgt
     1921 tcagtctgcc gggcatcggc cgattcacct cgaaagaagg cctcccaaat atccattcct
     1981 gcgatccgcc gttcattgtg ccccgaaacc gtcacggccg acaattgcct gccaaaaatt
     2041 gtcaccatgt gccaaaaatt tgtatgtcgc cagcgtccgg aagatatgta atgacagctt
     2101 aacgtcccgt aatcatgcaa aacagggttg tggcacgatg gttgcatgat atacgggcaa
     2161 gggttgcgcc ggattggccg cacccggccc gaggtccggg caaccgtttc aacgtttgac
     2221 aaactatgga gggtcaaatg cttaacaaat tccgtaagtt caacttcaag ggcttcacgc
     2281 ttgtcgaact gatggtcgtc gtcgccatcc tcggcatcct ggccgtcatc gccgtccccg
     2341 cattcattaa gtacatgcgc cgcgcgaaga ccacggaagc aatcgacgaa ctggacaaga
     2401 tctacaaggg cgcgtcctac tactacaccg ccccgcacgt cgatgccacc ggcaagaaga
     2461 tctactgcca gttcccggct gaccagggca cgaccccggc cgcttcctgc tgcgaagcgt
     2521 tctccggccc ggacaccgac aaggacgatc gctgcgacag caactccctg gcatggaacg
     2581 agaagacctg gtcagccctg accttccagg tcacggacca ggtgtacttc gtgtactcct
     2641 gggatcagaa cgacgaggtt atgtccgtcg ccaaggcgac cgcgaacgcc cacgccgacc
     2701 tggactgcga cggcacgcag tcaacgttcc agcgcatcat caacggtgac cccaaggcca
     2761 cctacgcgga atgcgcttcc gtcggcgcct cggccttctt cgtggacaac gagaccgaat
     2821 agttccgacc tgatcgcttg acagaaaggg cgccttcggg cgcccttttt gttatgtggc
     2881 gcgcccaggc tttccatctt cggcttccct tttgccttct gattaatgaa cgggactttt
     2941 cataatgccc aagtcggcgc gggaacggta tgataggctc gtcgaatttg ttatcccggg
     3001 actgaaagca tgaagaacac gttgtcggtg cttatcgccc tgagccttct gttccacgcc
     3061 gctcaggccg gggaggggcg ggtgcccgga tccggcgagg acttcacgcg caaggacagg
     3121 accgagcttc tgttccaaac caagtttcag ttcacgccgg aaaacgttcc cgtcgtcacg
     3181 gtaggaattg tcgagggagt cggggatgtg gcattttctt cagaggggcc gcttgttttt
     3241 cagccggagg gcgagggagg gccgaccgtg cgggttggtc gttcgaggat cgattgcatc
     3301 gcgacaatcg aggattcgag gccggctgtc ataaggtact gggcggccct ggaaagggtg
     3361 ccggcgatga gtctggcaac cgtgcgtgaa gcgaggaagc gttggacgga taagggcctg
     3421 aacatcagga cgttcgagca gggaagcgtt ttcggctttt atgggcgggt gctggacaac
     3481 aggcgtgtgg tgattgtgga ggacgttccg tttgcggaca gggcggcggc catgaagcgc
     3541 cttgatgagc ttacttcgtc acacggtctt tcaacgcccg aaccttacga ggaagtgctt
     3601 tcacgtcctg acgggagggt tcgcgtgcgt tgcacgaatg ttgatgccgc gatggcattt
     3661 cccgggatcg tcacggtaag cccttcggca ggaaagacaa tccgggtgag gaacgtggaa
     3721 ttccataagc tgtacggcca gggaaagtcc gaggaccgat cgtaccgcgg caacctgata
     3781 cttaccgccg accggagcgg ccggcttgcc gtggtgaacc agatcgatgc cgaaactttg
     3841 ctgaaaggac tggttccttc cgagatattc acggagtcgc cgatggaggc attgaaggcc
     3901 caggcggtcg tggcgcgtgg cgaactgttt gcgaagctcg gcaaccgtca cacggcggat
     3961 ccttacatgg tttgcgccga cgttcattgc cagtcgtacc gaggtctgga acgcgagcat
     4021 cccagggctt cacgggccgt cgaggagacg gccggcatga tggcttttca cgaaggcgcg
     4081 cttgtggatt ccgtatatag ttcgtcgtgc ggcggtcatt cggaggccgg ggccaatgtc
     4141 tggcagggga gcaatcaccc gtatctcggg ggaacaccgg atctcccaga caagacgagg
     4201 acgcgtcttt tcaaggatgg ggtgaccgag aaggccgtgc gggattacat cgataatccg
     4261 ccgaaggacg ccttctgcgg caggtcgcga tttggcaagt caaccttcag gtggagctcc
     4321 acgctgtcct tttctgccat ttcggaaggt gtggcaaagg aaacaggtgt cagcatcgga
     4381 cagttcaccg aattcgaggt acttcatcgc ggcgtttccg gaaggataac caggttgcgc
     4441 gtaaagggat cgtcaggttc ggtcgatatc gcgcccgaac tgcggatccg tcgggcgttg
     4501 ggcagtttga aaagttcggc cttttatttc gatgtcggca acggttccgt gacgttccgg
     4561 ggcgccggtt tcggacacgg tgtcggaatg tgccagaacg gggcgatcgg gatggccgat
     4621 gctggaattc gctatgacga gattttgaag cactatttca gcggttccga aatcgtgaag
     4681 atctattgag acctctgctg ctctgacccg tcaccctccg aagtctggtg gtccacaaga
     4741 ccaagcagca cccacgattt gccgcgggcg aattcgcgca cctttttaag gtcttccgcg
     4801 tcaatgtttt cggttttcag catcccgtat gcgagttcag gcgttgaaac ggcccacggg
     4861 caattcacgt cgcgcatcaa ttgaagacgt tccttcaacg ttgtcggttc cgtcagatat
     4921 ttgtagtgcg ccctgatctt gtcaaggaat cccggcgtga cttcgtggtg cgcgatgaac
     4981 acgtgcgcgt ccgtttccgc tgcgatgaag agggatgtcc gcggatccga aatcacgttt
     5041 ccgtccttca cccaataacg tacggcgtcg agcatttcgt acatgtcttc cggcacgtag
     5101 cctggattgt ccggaacgac ctcacgattg atgtgataaa gcggggccgg cagcgtcgtg
     5161 gccagcatca ggacaaccgc cgcgacacga atccgtttcg atgcgattcc agcgacgtac
     5221 cggacaccca tcacgacaac tatgaggtgg acaccttcca tgcatctgcg ggcaaacggc
     5281 acaagaggat cagcgtatgc caggacgagc gccgtgccga gccacaccat gccgaacatg
     5341 atctccgggc tttcaggcac ccttgtcttg accatcctgg ccacaaggag gcctgccgcg
     5401 accccgggca agccgaagcc gaaaacgtat tcccatatcg ggggcgaggg catgtggttc
     5461 tgggatgccc atgccgcggc gaccgggttg aagtggttct gcagcagcag ggcgatgacg
     5521 gccggtgccg agatgccgat tacccaccag aatgccggat ccaactgcac tgtgccgatg
     5581 gtgtttcgct ttttcagcca gacaatgacg acacgcatta ttacgattgc ggcgaggatc
     5641 gttcccgcgg tgtagggatg aaccatgaag agcagggcca tcagcacgcc tgaaagaacg
     5701 taaagcgact tgatgaagaa ccggttggcc ttcatggaaa ccagaaagaa gagaagcacc
     5761 agaatcaggg agagcacgaa atgcggatag tgaagcatcg agggccagga ccatagttcg
     5821 gtcatccagt ccgccgaggg aaccctgagc cccacggttt ccagccagcc aagtcccccg
     5881 ccgatcgcga tgatccacca ggccatccac gtccagcgcc tccccggaag catctttgaa
     5941 agcaccagga aaagaagcgg cagatacgcc agtgacagca tcatccgcat gaagttgtat
     6001 gaaaacaagg tgttcgatcc gaaaaccgcc ttgtagagtc cgatcagcat ccaggcagga
     6061 ttcggcaaca ggcgggggtg gggctcgacc gtcatcctgt tttcaacaag gatgtgcccc
     6121 tgggcgtgct gattgaacca catcatgtag ctggcggcat ccggaaggtt ggtcgatatc
     6181 ccgtagaaga ccgttccgtc aggtcgccgt tcgtcaatca tgtggtacgg aatcatcgtc
     6241 gaaaggacga ccgccgccga cgcaaccaca acaaggacgc cgttgaggta cccgaatcgc
     6301 ttaagcatcc gcacccgcca tcgcaaggaa atcaagttcc gaaatcaccg cgacgccaag
     6361 ttccctggcc tttgcaagtt tgcttccggc gtccgagccc gccacaacga aggacgtgcc
     6421 ccttgatacc gaacctgaaa ccttcccgcc aagggaaatc accagatcag aagccttgtc
     6481 gcgtgtcatt ttctgcaggc ttccagtaaa gacgaaggac ttgccttcga aaggtccgcc
     6541 gccggccgcc gactcgttca tcatcgtcag tcccgcctgc cgaaggcgtt cgatcagatc
     6601 caggttcctt ctggactgga aaaagtcgaa gatgctggcg gcgacgatcg gcccaacgtc
     6661 ctggacctgc cgcagatcct cggttgatgc ggcagccagg gcagccaacg atccgaagtt
     6721 cgatgcaaga tcccttgccg tcgtttcgcc gacaagactt atgcccagtg cgttgattaa
     6781 ggccgcaagg ggccgccgtt ttgacgcctc gattgccgcg acaagcttgg ccgcggattt
     6841 gtcgcccatt cgatcgagag ccgagatctg tgtaaccgtc aaggcgtata tgtccgaaat
     6901 ctcgtgaatc aggccggctt caatcatcag gtcggccatc ctttcgccaa ggccttcgat
     6961 gtccatggcg cgcttggatg caaaatgtat gacgtgttcc ctgaactgcg cgggacagga
     7021 aatgttcgtg cagcgccagg caatttcatc ctggcggccg gcaaccggct ccagacagga
     7081 aggacatctg tccggcatat tgaatatggt ctcgtttcca tcgcgttcac ttatcaccgg
     7141 ccggacgact tccggaatga cgtccccggc gcgttgcacc tcgaccacat cgccgattcg
     7201 gatgtccttt cgacggattt cctgtatgtt gtggagcgtg gcgcgccgga ccatgacgcc
     7261 gccgaccttg acaggctcaa gctctgcaac cggtgtcagc acgcccgttc gtccgacctg
     7321 gacaacgatc cggttcacga ccgtcttttc ccggcgtggg gggaatttca aggcaatagc
     7381 ccacctgggg ctcctggaaa tttcgccaag ttcgcgttga agttcgaagg agtcgacttt
     7441 tacgacggcc ccatcgattt cgaacggcag cgaatccctt tcgcttccca gccgcttgaa
     7501 ggcgtcaaga acctcggccg cgccgcgtgc cggccgaatc agcgaggaga cctgaaatcc
     7561 gtattccctg agtcggcgca gaaggtcgcg ctgcgagagg atttcccggt catccagtct
     7621 tccaaccgtg tatgcgaaga accgcaatgg tcttgatgcg gtaaccgcgg ggtcgagctg
     7681 gcgaaccgag ccggccgccg cgttccgggg gttggcgaag gcggacattc cagcatcaag
     7741 cctttcctgg ttcatctggc ggaaaggttc cgtcggcatg tagatttcgc ccctggcctc
     7801 gaaaagctcg gggagtggcg gcgaaacttc atcgaaaagt ccgggctcgg cgttgaagga
     7861 aagcgccttg aggcgttgcg gaacgtcctt gatggttctg acattcgcgg tgatgtcctc
     7921 gccgacctgg ccgtcgcccc gcgttgaagc ctgcaccaga acgccgtcac gatagactat
     7981 ctcgcatgaa aggccgtcga ttttcggctc gaccacgtaa tcgacggact gcaggccgag
     8041 agccttcctt acgcgttcgt cgaacgccag aaattccgaa tcgtccatag cgttctgaag
     8101 cgacagcatc ggaacgttgt gtacaacctg ggcgaactgg cgcgcggggg cggagccgac
     8161 gcgcctggtg ggtgaatccg gtgtcgagaa ttccgggaat ctggcttcca gatcctggag
     8221 ttcgcgaaaa agtgcgtcgt aatccgcgtc ctcgatttcc ggctggtctt tttcgtaata
     8281 aagacgggcg tgccggctga gttcggactg cagccatctg attcgatcag acgcctttcc
     8341 tgaatccatc caacgtcccc cgggacagac ctgcggcaca caccgcaaaa gcatatcaca
     8401 gcgtcgcact tttcggtaag aacagcggaa ggttcggtgc cgtccgggcc gattttcagg
     8461 gggttactat tcggtatagg ggatgaactc gacttcctcg ccccactgct gggccgattc
     8521 cggaaccagc acggcaccgg cggccgggct gtggcgaagg aagtggttca ggaaggaaat
     8581 gccgtaaagg ttgatgacct tgtgcgcctc gcgccagtca aaattgtccg ggccgcaccc
     8641 atcgcgaatg gcatcgtcgg cgtcttccca gtacgcctgc aattcctcga ggttcatcat
     8701 gcaggaatcc gtgaaggtgt agtgtccggc ccgtggcagt gaaagaagcc acttcggcgc
     8761 cggctgcgtg tcaaaaggat ccctctgctg cggctggtac tgaagagtct tgtcctgttc
     8821 accggccatc atcagtgtgg ggatggacag ttcctcaatc ggggtgttgt tgaagtagat
     8881 ggtgaaggcg ttgaccatgt cggccgccgg ggacataggg atgatgcagt cgatcctggg
     8941 atcgaccgtg acgatgtagg acgtcagccc gccgaaggaa tggccggtgc aagccacgtt
     9001 ttccatatcg accaaatcgt agaattcatc cgcggtgtct tctcccttgg cctcgatcca
     9061 gtcaagcagc gcgaagatgt ccttcggcct gtgctgggcg gaataaagaa gctcggaagc
     9121 ctcatagccg tcaaccagga tttcgttcaa ggtattgaac tggtggtcgg cggagatgac
     9181 tatatagccg tgcgacgcaa gctggatggt gaagaagatg ctctggaagc ggatgccgaa
     9241 cgcaccgtga gagaagatga cgaccgggaa gcggccgtcg ccatgacgca ccggggcatc
     9301 aaggacggca tccaccggga agcgaccgat ttcgacgcct tcgtactttt ccaggacgtc
     9361 cgggccacac tcctccttaa cgtcgtagat gaagggatcc ccgccgcgga attcttccgt
     9421 agtggggaac cagacgtcga tcttcagctt cctgggcgaa ccgtcctcgt tgttattgtc
     9481 cggattcgga tcgtaataga cgaactgtct gattccgacc gggaagggcc cgaacgacat
     9541 cggatccggc gccgcttccg gtcccgcgac gcacagttcc ccgacgcatc ccgggttcgg
     9601 gttttcgatc atgttgccgt tgccgtcgtc gatgcactta tggggagggc agggcggcgt
     9661 aatcgaatcg ggctgggcat cgaccagggg cgtgtcatcg gccgcgtcag cgggcgttcc
     9721 ttcatccgga tttgccacgt ccgggacgac atcgttgccg ttgtccgggg ttaccacgtc
     9781 gggcgtgccg ccctcgtcgg tctggctgga accgtcgcca cagccggcga ccatgagcgt
     9841 cgcaacaagc attgaagctg ccaaaattct tgaaatcatc aacagacccc cgaatttgcg
     9901 aaagaaagac cgaagggaac cgcgaacatc cggaattttg acgaatccgg ccgtccgctt
     9961 caagccgcag aatttctaac cgtcaatgcg tgcaaatgca acaaaaatca accggccggg
    10021 ataacgcccc cggtcgtcaa ccggcatgtc cggaatggct gatgcattca agcagctcgg
    10081 cgccgaattc atcacatctc caggcgtgga cgccgggaac ctggccgagt tcggcgatcg
    10141 atgcggggaa tgccgcggca atcgccacaa tcaggcgctg cggcagcaga agtccgggat
    10201 caagtcccag ccgcccggac gcccccgcgc gccacttctt cagcctgtcg atggactcag
    10261 ccacccgcgg gtcaaacggt ggccgtcttc ttaaccgggg gatttcgact ggcggatccg
    10321 aaaggccgtc gcggatggca gcaagccagg gttcgggatg cgatgccacc gggcgcggca
    10381 ccgcccggat gcgctccagc gcggcggggc ttcgaggcct ggaaatggcc atcctgacaa
    10441 gtgcatcgtc cccgatcacc ttgaagcgcg ggcgatccag cttcagagcc acggcctcgc
    10501 gggcggcaaa aaggcgcttg agtatggcga ggtcggagcc gtccaggtcg caggccccgg
    10561 ttgccttcag gaaatcggcc ggggcgggaa gaggcgatgc ggcgtggata tcagcgattg
    10621 ccgccgattc ctcgatcacc catcgaagcc gcccggcctc ctgaaggtaa cgaaccatgc
    10681 gccggtatat ctcgaccagg taattgacgt cgccggcagc gtattcaagc atgttggccg
    10741 gaagcggccg aaccgaccag tcggcggtct gtggcccctt cgggatctca tgcccgaatt
    10801 cccgcaggat tacggcggcg agcccgaccg acggccaccc aaggaagcgc gcggccagcg
    10861 cggtgtcgaa gacgtttgaa aacgaccacc cgaagtcgcg cttcatcgat gtgatgtcga
    10921 aatccgcccc gtgaaagatc ttggttaccg atggactggc cataacggcg ccaagtggtc
    10981 cgggatcaac acgagaaagt ggatccacca gaatcgtccc ggttgcatcc gatgcaatct
    11041 gtatcaggca aaccttttcc cggaagtggt acatgctgtc cgattcggtg tcgaaggcca
    11101 cgcatggctc gtccagcagg cttcgggcaa gtgaatcgac caggctgtct gtgtctgcaa
    11161 aaagaggcat cagggaatcc ggcgggcctg ctgggattac cacttcatcg ggatgaagac
    11221 cttcacgatg acgttgtagt agtcttccat ggccagcgca ttgtatccgt caacgatctt
    11281 gcgccacgag aaatcgacct ggacgggcag gtacaggggg atcagggaga ggctggcgcc
    11341 gacctgaatt gcctggatgg tgttctgtcc aagcaactgc aggctgcgaa ccatctgata
    11401 gaagtaccag tcgccctgga tttccggttc ctggctgtgc tggacgccat aaccgaagtt
    11461 caggccgagg attgccagct gaacggaaag ctggcccatc cagcttacgc cgaagccggg
    11521 ggtgtaggag tactgaccct tcaggtgacg aagatccggg aactgcgcaa agacgttcgg
    11581 atcgttgaat gcctgaagga tcgagcggtt tggctcggtg gtgaagttgg tcgggtaatc
    11641 gcgcttctgc tcgaagaagt atgccatcga gaactcggag ctgagcacga tatcgaccca
    11701 gtgcttgtac ttgaagactc gaagatccca gccgtttgta aagccaagtc cccaggagcc
    11761 tgttccgacg tcgatttccg gcccggttgc catcgtcagg ttggcgtttg ccgaggccac
    11821 gcgtccggtc ggaaggaagg cccggacggt gagcgaagtc gaaagatgct ttgtacgcca
    11881 ggggttccac gagaaaccgg tattgatgtc ggccagaacc cagtcggcat cgtagcgcag
    11941 tgccggaacg gggcgtccga gctgcgggat gaactggtag agcttggcga tacccatgcg
    12001 gcttgcgtag gtcttgtcgt tcgggtcgtt cgggttcttg accggctgct tcgaagagtc
    12061 caggaacttc gggtcaatcc tgacgtgcat cgactggaac ggcagttcga aataccagtc
    12121 gaagttgcca aggataccgt acgatgcctg gaagacgtgt cccgcaccgg caccgttcag
    12181 gccaagatcc aggtacccgc cgggcatggc gatcggggcg attgccggtc cgagttcatg
    12241 cttgtcgttg taacggctgt caaccgtcat catcgagtga tcccacttga ccgacagata
    12301 gcgcttcgga agctgcgcgg tcacgccgaa gcggtcgtcc ttgccaagcg cttccagttc
    12361 cttgtcgaaa aacgccttgt acggatgaac ctttttcttc ggcttgacgt cggcagttgc
    12421 cgaaaccgtg tcgaccggct catcggccgc gggttcaacc tcgacgtagc tcggttcggg
    12481 gttttcgtcg gctgcaggtt cggcctcggg cacaagttca tcaacggcct cgtcggaagc
    12541 ttcctgggcc gaaacaggcg ccgcgcagac tgcaacagcg aaaaatgcga cggcggccaa
    12601 tgtcttcaga agtgtcttca tcggcacgtt cctccttatg ggcagacggc agcgccgcac
    12661 tcaagcaggt ttttaaggga tcgcggcagg catgccttaa tcgggttgcc atcgccgtcg
    12721 gtcttctggc tgtccaggca ggtgtaatcg acgccgcacc tgcagtcgat atcggattcg
    12781 cacggggcac cgcacaggaa tggaaccgtg ggatccagag cggtgggatc caggcagatg
    12841 gcgccggtgc cgcagtccgt gttacccgag catccgatca tcgtgcacat cccgtccggg
    12901 gcggtcgcgc ccggccatac cgcgttcagc agatcggtgg tcgcgcaaag ctgggtgacg
    12961 cattgagtgt cggtcgtgca cgggtcgccg ccgactcggc cggtctcggg gaacccgttc
    13021 gggtcgaacg tgcattcgcc cccgggctcg caggtgcacg tacacggctg cggttcgcag
    13081 gtgcatgtgc actccgggac gtcctcggca acatcctgga cgacatccgg ggtcacgttg
    13141 cccgtgtcct ccggaacgtc ggcctcggtg ccctggtcgc cccagccgtc ggccatgaag
    13201 ccaaggtccg cggtctggct ttcataggtg tagcatcctg gagtcaggat cgccgcagaa
    13261 accaacaata ccaatatcga agcggttcgc ctcatcgggc cagccccctg gtaaaaagct
    13321 ccgcacgcat ttgacaacgg ctgcatgttt gtgtcaagaa gttacggtgc tgtcagcatc
    13381 ggttttgctt tccttttttc agatacgtcc gtcgtggtat ctgatagact cggcgcgggc
    13441 cgggcgaacc gtcgccgacc ccggattttg gtttcgaatc gattcaatgg agtaacagat
    13501 gctgcagttt gaactgatga attccttcaa gaccagggcc cgaaagaacc cgaagacgat
    13561 tgttcttccg gaaggcagcg agccaaggac gctgaaggcg gcggccataa ttaatgaaga
    13621 gaagctggcg acagtcatcc tggtcgggca ggaatccggc atccgggctg ccgccgcggc
    13681 cgagcacgcc aacataaggg atataaggat tgtcgatccg gacgcttccg aactgctgcc
    13741 gggatatgtc cagaccttct acgaacttcg gaaggccaag ggcatgagca tggtcgaggc
    13801 cgacaggatg atgcgcgatc cggtttattt tgccacgatg atggtctatg ccggtgacgc
    13861 cgacggtctt gtttccgggg cgattcattc gaccggcgac acaatccgcc cggcgcttca
    13921 gatcatcaag accgcgcccg gaatcacaac cgtttcatcc gcattcatca tggttgttcc
    13981 ggactgccag ttcggtgacg aaggtctgtt catattcgcc gactgcgcgg tcacgccgaa
    14041 tccggatgcc gagcagcttg ccggaatcgc ggtttcatcg gccgagacgg ccagggtgct
    14101 ttgcgacatg gagccgcacg tagcgatgct ttccttttcc accaagggca gcgcgaagca
    14161 tcccctggtt gacaaggtgg tggaagcgac gcgcattgca agggaactgg cgcccgaact
    14221 cgatctggac ggggaactgc agctcgacgc cgcaatcgtg ccgaaggtcg gcgcattgaa
    14281 ggcgccagga agcccggtcg ccggaaaggc caacgtcctg gtcttcccgg atctgcagtc
    14341 cggcaacata ggctacaagc tggtggaacg acttgcgaag gcttccgcaa tcggcccgtt
    14401 cctgcagggc ctgcgcaagc ccgtcaacga tctttcccgt ggatgttcgg ttgaagacat
    14461 cgtcaacgtt gttacaatca ctgccgtcca ggctggcggt gtcggtcgct gacggttcgc
    14521 gacgcttgtt ttccaggtgg cggccacgtc gtgccgccag cattgattcg gagatcaaaa
    14581 tgataattct ttcgttgaat tcgggtagtt cgtcattgaa gttccagctt ttcgactatg
    14641 acgaaagcat cacgatctgt tccgggctgg tcgagcgtgt cggcattgcc ggcagtttca
    14701 tcaagttcag caattacggt ggcgagagcg tcaagatcga acacccgtgc ccggatcaca
    14761 gggaagcgat gaagctgctg ctttcaacca tcacgggaag cgagcacggc gtcatcgaca
    14821 gcctggacag gatagcggcc gtcggtcacc gcatagttca cgggggcgaa aagtttacga
    14881 agtcggtgct gattaccgac gaagtcgtcg atgtcgtgaa atcactttac gacctggccc
    14941 cgctgcacaa tccgccgaac atccttggcg tcgaggccgc ccgggaggtc atgcccgggg
    15001 ttccgcacgt tggcgtgctg gacaccgcgt ggcaccagac aatgccggaa catgcgtatc
    15061 tttatgcgtt gccgcggaac tggtacacga agtacggcgt gcgccgctat ggcttccacg
    15121 ggacctcgct gctttattgc gccaagcgcg cggccgccct gctgggcaag gatcccctga
    15181 agaccagcat cgtcactttg catattggaa atggcgcgtc cgcgaacgct gtcaggaacg
    15241 gcgtcagctt cgacacgtcg atggggctta ccccgcttga gggtcttgtg atgggaaccc
    15301 gcgccgggga tcacgacgcg gccatcgatc tgatgatcat ggaaaaggaa gggtttacgc
    15361 cggcccagat gaattcgttt ctgaacaaga agtcaggcgt gttcgggatt accgacgaga
    15421 agtacgtcga tcgccgtgac gaggaagacg cggcggaagc cggcgacaag ctggcgcagc
    15481 ttgcactgga catcgaggtt catcgcctga agaagtacgt cggcggatat atcgccgctc
    15541 tgggcggggt cgatgcaatc gtgttcaccg ccggtgtcgg cgagatgtcg ccatacatcc
    15601 gcgaacacgt ctgccgcggg ctggaatgca tggggatcga gatggacttc gtgaagaacg
    15661 cgccttgcaa gacgcggtcg gccgaatgcg accttgcgac cccggaatcg aaggttcgca
    15721 tcttcgtgat tccgacggac gaggaacgcg tcattatcga ggacaccgtg gcgataatga
    15781 atggaactta cgacgaccac acaaggttcg tctatccctt ccagcttccc ggatacgtca
    15841 acaaggaacg cgaggccgcc cttgcccgcg acatggaaaa gaagtcctgg ctgaaggact
    15901 gtgtggttcg tcccccgaag gcgtgacaag cctcctcccg attattccgc cgagatgttc
    15961 agtctgattt cccttatcgg cattgttcag cgatagctgt gcaggccgcc gatgaagaag
    16021 ttgacgccaa ggaacgtgaa cagcacgaaa acgaatccga cgagcgcgac gacagccatc
    16081 ctccgtccgc gccagccacg catggctcgc aggtgcagat aaagggcata gacgagccac
    16141 gttaccagcg accatgtttc cttcggatcc catccccagt aacggcccca tgcgaaactg
    16201 gcccagaccg cgccggttgt aagtcccagc gtcagcagcg gaaatgccag cagcaccagc
    16261 ctgaaggcca gcaagtcgat tcgcttcagg gatgccggat cggcaccggg cacgaaagac
    16321 cacgcaagct gcagtgccga ggcgaccgtg gcaaccgcca gcgccccgta acccagaaag
    16381 tacgatgtaa cgtggataat cagccagttg cttttcaagg ccggcatcag tggccgaatc
    16441 gacgaatcaa gaacggacgc gtatccaagc gccagtaccg cgaccagggc cgatgcagcg
    16501 ccgaccagcc tggttctgaa agtgagttcg acaaccagcg cggccagccc ggtggcgaat
    16561 ccaataaaca cgaacgactc gtgaaggttt gaaaggggcg ggtggccggt cgcgagccag
    16621 cgcaggatga tggcgacaac ggtcgcgacg gtgccgccga agagcgccgc gatcccggaa
    16681 aaccggacga gccggttcct gaatatgtca cctgcaagaa gtgcggccgt tgccacaatg
    16741 aaaagggcga agcccgtatt tatcgcgttg ctgattttga aaacctccgg tgaagcacca
    16801 ggaatacacc caggttcatc aatgcgattc ccaggaaaac aattgccacc ccggggtcat
    16861 aaaccacctc gaggccggtc catccggggc gcgcgacgtc gaagcttgtc tggtaaatcg
    16921 accagccgcg gtgctttttg ggctggttga ccttgattat tgcggaagtc gtggacccgt
    16981 ccttatccag gatggtgacg cggctggcga aatcaagcgg cttcgcatcg tgcctgtaga
    17041 ttatgtcgat gttgccatcc ttcgacatgc tgaattcggg atgggccgaa aacacccacc
    17101 gcttctcggt ttcacctgcg tggtggatca gcaggagcat cgccggattc gccgggttgt
    17161 cgctgcgcga aatgattccc tgttcgcgat cgacaaggag gtccgcgacg aacctggata
    17221 cttcgattcg gtaatcggtt cccggaacgt cgtaggtgct gccgggctcc accttgatgg
    17281 acgtacggac gcctgcgtcc atcacgccga catgaagcag gtgaggcgcc gacctgtccc
    17341 agtcgatcct gaatt
//
LOCUS       DAOPPA010000009        16595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4155_length_16595_cov_4.245707, whole genome shotgun sequence.
ACCESSION   DAOPPA010000009 DAOPPA010000000
VERSION     DAOPPA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_16595_cov_4.245707"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKH54_00670"
     CDS             complement(<1..1212)
                     /locus_tag="PKH54_00670"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOC98427.1"
                     /translation="MKLVDAQTMRSIDQTAINSIGIPGVVLMESAGRAVADAVLSIAA
                     SHGTDRVLVVCGPGNNGGDGFVAARHLIDSGLEVVVVLLVGRSRYVGDALTNLKALDW
                     FGATVVEAPSGIPDNLVAWAADAVVVDAIFGTGLVRPVGGVFADTIDILNTLQGPAVA
                     VDIPSGIDPDTGRVAGTAFEADVTVTFGAARIGHYVWPGRGHCGDITVAPIGIPRALI
                     ERAPGATLLQPADICPGFLPRHLDSFKNSFGHVAVIGGMPGRTGAAILAGRASLRAGA
                     GLATVVTCDVSASRIDGSVPDLMVEPAFRLTDGDSGRGPWVGCEIASIHSVLRQKSAV
                     VLGPGLGLLPGTESMIGFVLDAGLPAVIDADALNILAAGRLDMPGASCILTPHPGEAA
                     RLQKTDSGIIQA"
     gene            1376..2017
                     /locus_tag="PKH54_00675"
     CDS             1376..2017
                     /locus_tag="PKH54_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98428.1"
                     /translation="MKRLLAFGAAAVAAFAAVGATAQDAPAITGQKAEAGAEDSQKTG
                     VILLTPAEGQVFTVDEDGRIYEKRGYKGVVPGVRDQAEIPGRAPAPVASADEEVPILI
                     NWIGFQPYEAFSRVFIQLSGEFEFSVTRPDPLRIEVLLPGAMPGTKNDMNGLITRWFP
                     TNVDRVEVAMVPSSETLPSGGIQVGVFLKKAVGFLYRREGGYVFVDVETKVEP"
     gene            2080..2910
                     /locus_tag="PKH54_00680"
     CDS             2080..2910
                     /locus_tag="PKH54_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98429.1"
                     /translation="MNRKSFRIPFAVVAVWLVVMTASVCHAKVGVGKLLSNLSGSGAA
                     RIRAQAALALSSFDDNPAVVKALVRALEDPHVMVRSSAARALMAIAPAEAFADICRIG
                     AQEKEPFALNWIQKAAARSAGASTKVLVNVDGLECVGADNRMMVSQKLRSAFLQKLFA
                     YDGYSIGVSLDFSGDASADEGAGVELDIVGSFTVDGNKQATSGKLDVRAVTTEGFVVW
                     STVIETIDVVQGKAPEIKDPFADEFTIREEGEDARLVAATEAGTRAATRLNVELRGVE
                     "
     gene            2948..3595
                     /locus_tag="PKH54_00685"
     CDS             2948..3595
                     /locus_tag="PKH54_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98430.1"
                     /translation="MSDKSQVPCGCQEGGKYQRSAVFIDRRFQTRYLLLIAGTALLIM
                     VVLGVMYAGVLADQRELLGINAIAGDTVVSGDDAALDAALSSSFRSEDDLRLAALFVS
                     AAILVGLLSWVAVRMTFRVVGPIRAASNMLRGIRCGDDSSIRHFRKGDEFMFLSRDII
                     DLRDSIREREALMKRMLDQARLEIERAGGDPAIAAEIGRFLSSDEGRFADNNRED"
     gene            3595..4590
                     /gene="ribF"
                     /locus_tag="PKH54_00690"
     CDS             3595..4590
                     /gene="ribF"
                     /locus_tag="PKH54_00690"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOC98431.1"
                     /translation="MRFVRGFEGFCGSVSSGTVVAMGNFDGVHLGHAQILSNVVNSAR
                     ASGLKSCFLSFHPHPLRVVAPERAPGMLQTIEEKLISLSAYEPDFVLVVPFSWEFSQT
                     PAPDFVDKYLLGLLDCRELHVGADARFGRKREGDVSMLRRLSSGGMFRLSVTEDVQVG
                     EHRVSSSRIRRSILDGDMADAVACLGRPWTIAGEVVHGAGRGRKMGFPTANIAADNSI
                     LPSAGVYSGFSRIDSRFLPVAVHIGPIPTFGVKNPVVEAHIDGFEGDLAGRRLRVHLV
                     SRVRGIRCFDGTRELVAGISADVDAVRSQVLELLDNRASIAYKVARFDPEGGDCW"
     gene            4617..4805
                     /gene="rpsU"
                     /locus_tag="PKH54_00695"
     CDS             4617..4805
                     /gene="rpsU"
                     /locus_tag="PKH54_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOC98432.1"
                     /translation="MEVQVQDNDVGRALSALKRKMANEGLYKELKKRRFHEKPSISKK
                     RKHREADRRRKKISPGGH"
     gene            complement(4857..5564)
                     /locus_tag="PKH54_00700"
     CDS             complement(4857..5564)
                     /locus_tag="PKH54_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC98433.1"
                     /translation="MAFLIERPIPERPYFKVGEVAQILGVAPSTVRYWQKEFPDHIRP
                     ELSMAGQNVFSRNDVITMAVIMNLVRIDGVSLKDARVSLTRIIQEHGGKVQDIRLCRQ
                     FNLDLGLEQEQTGDRPPEVTDCDEDRQDVEPPESDCVQVPARDTDDAGGRDAADARHS
                     GQDAELLKTVRELQREIQLLEDENIRLREQIARGREDLARSGLESTEYAARLRILQEQ
                     VYAIAGGILQSLDCGRD"
     gene            complement(5598..5876)
                     /locus_tag="PKH54_00705"
     CDS             complement(5598..5876)
                     /locus_tag="PKH54_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOC98434.1"
                     /translation="MTKADIVEGVYERLGGFSKKEASAIVETILDVMKEALVNGEKVK
                     ISSFGSFAVRSKNERMGRNPKTGKPLEITARKVLTFKPSQVLRSILNP"
     gene            complement(5896..6252)
                     /gene="rplT"
                     /locus_tag="PKH54_00710"
     CDS             complement(5896..6252)
                     /gene="rplT"
                     /locus_tag="PKH54_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOC98435.1"
                     /translation="MARVKRGFKRRQRHNRVLKMAAGYRERRRTAYRHAVEQVERSLA
                     YAFAHRKQNKRNYRALWIARINAACRMNGTRYSDLMHAMKVNGIDLDRKVLADIAVSD
                     PTGFTQIVKQTVGATA"
     gene            complement(6270..6467)
                     /gene="rpmI"
                     /locus_tag="PKH54_00715"
     CDS             complement(6270..6467)
                     /gene="rpmI"
                     /locus_tag="PKH54_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOC98436.1"
                     /translation="MPKMKSNRAAAKRFQMTASGQVKRGKAFKSHILTKKAHKRKRNL
                     RQETLVAPADLRKVKRLLPGY"
     gene            complement(6448..7041)
                     /gene="infC"
                     /locus_tag="PKH54_00720"
     CDS             complement(6448..7041)
                     /gene="infC"
                     /locus_tag="PKH54_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC98437.1"
                     /translation="MIQQGQQQKTAENEARINRRIRAREVRLIGSDGSQLGVVSITQA
                     LEQAEEEGLDLVEVAPDSRPPVCRIMDYGKFKYQKKKRQAEAKKRQVQVVLKEVKIRY
                     KTDEHDLVTKINQARRFLMEGNKVKFVMFFKGREVQFAELGYHVMERVAADLVEVSVM
                     ERPPRMEHRILAMYFASKGVVKDAGKESENEDAKDEE"
     gene            complement(7111..7185)
                     /locus_tag="PKH54_00725"
     tRNA            complement(7111..7185)
                     /locus_tag="PKH54_00725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7151..7153),aa:Val,seq:gac)
     gene            7449..9083
                     /locus_tag="PKH54_00730"
     CDS             7449..9083
                     /locus_tag="PKH54_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC98438.1"
                     /translation="MLNRLVLRRRVVCPPLLVCFVCSMLWMPFAGFAQSAEGRGPAIV
                     LVIPPQKTAEVPESVASAVEQNLKGLLDIDSNMKMLYDGVRDPGPEYDIPDTTPKEPE
                     VQVKPLQENPKVAKALDSLATVRKMVEKKQYEGALSRLLNLNSTFGGLVKDMDSLSPV
                     LEALELTAASFINGGFAEEGAGAMRNLLTVQPDYAGAGQSDRVKAAVDRNRERLAAGG
                     DLIVQVKPDDAVVYVNGRLVGQGNQVVTGLKRGKHYVRVTGDTSFPDGGTVTVKPEGN
                     AVTVSYELKSRLVTIAPPPPPPVKQGPKPLVWYARTGLYAEPAFENDIKAAAANVFAD
                     NVLFVFIARGDNKFHIGAFVGNGLNGGVVEVEKAVVETDLSNLYIELLGLETRIQKAV
                     VDSSSMARIAAQPPIYRMVQAKPKPVPVVVVAPPPPPPPAPVAVEPAYQPYPAPYQQP
                     APAVDPGLPADFPMDLPSDFPMADTAPAAQPAYQQAASTTTVQEEDVVDEEPVYKKWW
                     LWTIVGVVVAGAVGGGLAWHFLNSDSGASVGGTVGW"
     gene            9095..10816
                     /locus_tag="PKH54_00735"
     CDS             9095..10816
                     /locus_tag="PKH54_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98439.1"
                     /translation="MNKTTLIAVGLGLVVLAAGCGGGDNPNLFSVQIKKAIAEKTYDG
                     ANPDPASGAVAVRLTVTGPGMDTRSKESDIGVGGSAVIGIPEFPEGDDRQFTFEFLGP
                     ANPLKNNERPVIGRGRSASLTVDADAVRAPDRIYVAPVNSMVRPTTVDDETGLVVSSL
                     PVTASRVGASMTELDDGRILICGGAQVRAEAETWYMPDDLNNLTDTCETYDPKTGLFT
                     EVANRMNVKRAYHQAVKLGSPDNPDGRVVLIGGYTVPDGGTITVTNLVDIFNPKTGLF
                     ISATSNEGQPGLYGGQGRALFTADLVNADKNLIVLFGGYSNVSAVGGTYDIVWVTEGS
                     VMTIAHAQLYNKADPAKGTVRYNHALAKVRKYADELVEGNGYQAYLLLGGENESGVLD
                     TVEPYVITCPGVDACKLQRQDQLVVKIPGGGRSMPAVAYDTLHNFLFIAGGFGAKGGK
                     SPTDRVEIFRVSNANFRENEVLSLGSKMGGMSIAEMDDGRFLLAGGWDGTKPVDAVKI
                     VSPKGPVIQNGQEIQTPAVKSAVPTMDAARAGHSMLTDQTGRVLIFGGIDAEKDSPEP
                     IFYNPGD"
     gene            10958..12535
                     /locus_tag="PKH54_00740"
     CDS             10958..12535
                     /locus_tag="PKH54_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC98440.1"
                     /translation="MAKLVFLSLILAPFPSSARSRQVLYFPPAVSGPALSTAVLQLAR
                     ERFENVLGSYGMYGVVPDEDALDRIGAADAGEFGSCRDASCRTRLTRLAGADVSAWLS
                     ISGRRGACVVEGVLSPAWSERKDIKARFEGGCDVVEIVDAVGVVAAMMCGRDPRATAA
                     PAKPEAVVAPGPTPRLQPLESTNAPPTGVVAQPQPPKAFKADELVGRIGFLTVETTPS
                     NASIEIDGKLVGKSPFQAEVQVGRHRIRVMLDKFFHEKTLEVDVGPDGRRVDVQLAPA
                     FGRLEVTSDVPGSLVTIDGRRVGTTPYVVDRALSGTYVIEVSNPSRRPSQRKVVVSDG
                     RTVIESFQLEQLVGVLSVLTRNYNDTPCSGHLSIDGKPAGSTPFKGTVPSGRHALEAT
                     CEGVSAKSVVDVPVDLRLSVVLRMPAPIVPAAAARPTGMEPVVRSEPARPPVVQDAPA
                     PVVNVKPDDGMVQSRAADVMNIEDERPKTVSKPLYKKWWFWTVIGIAAAGVAGGVAAG
                     VMSAGGGSSTASGIMTH"
     gene            12553..14304
                     /locus_tag="PKH54_00745"
     CDS             12553..14304
                     /locus_tag="PKH54_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98441.1"
                     /translation="MLDRGSCGVMFRASDLLRPALLAWVAACWLASCGGSEMAADNVN
                     LRLVSVSAKITYADANPDPENNVVAARVSVSGPDMSDVSSEFDFSLDAFQIPEYMLGM
                     RRQFSAEFLGPADPLKNDERPVVSRGRSVPIDVTERQTRIRSQIFVSPVNSFSSVVGM
                     DDISGEPVISYPINADRVGASMTELDDGRILICGGAKVRGPAVTWYMPDDLFTLTSSC
                     ETYDPETGIFEEVPDRMTVKRAYHQAVKLGSPDNPDGRVVLIGGYTVPDGGAITVSHL
                     VDIYDPRTGLFTSATSNEGQPGLYGGQGRALFTADLVNADKNLIVLFGGYSNVSAVGG
                     TYDIIWVTEGSVMTIAHGQLYNKDDPAKGIVRYNHALARVWRYGSELTGGKEYEAYLL
                     VGGENESGAIDTVEPYIITCAGVESCKLQRQDQLVVKIPGGGRSMPAVAYDLAHNFLF
                     IVGGFGATGGKNPTGSVDVFRVPEAGFREREYLSLGIDLGGMSIAEMDDGRFLLAGGW
                     NGTAPVDTVKILSPDGPVMFNGLEVVSPDVKSDVPAMDAARVGHSMITDQTGRVLIFG
                     GVDNDRDSHAPLMYNPR"
     gene            14379..16103
                     /locus_tag="PKH54_00750"
     CDS             14379..16103
                     /locus_tag="PKH54_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC98442.1"
                     /translation="MRLNEDGLFGESGRIRGMPGLPSFFLVMLLSTVINLAPGIAAAR
                     QKIVLYYAPTASGVRLSAADLGQMRDQFASVLGSFGLYRVVSEGEIPERLAPSDVRDF
                     RSCSDSECRVRISRLGSADVSAWLKISGRGGRCVVEGFLSPAWSDKGDKSASHEGGCS
                     GSQVAASAAVVAALLCEKEPGQAAIGTSGGGAVVPAAGSDGSTPAPVAPPAQVAVEPV
                     NKIQMLVQKTGFLTIETKPVGASIEIDGRPVGKSPFQDEVDVGRHRIRVHLDEYYRDA
                     TAEVDLEPDGKSLTLQLAPAFGKLEIKSREPGATVTINGNRVGTTPFVSERFLSGTYA
                     VEVSNPGRRPLRRQVVVSDGRTTSETFDLDVLMGVLSVLTRNTDDTPCSGKLFIDNRA
                     VGTTPFKDTVAAGNHVLKAICGDSSASSVVDVPVESRLSVVLRMGDKPSAAVAAAVPA
                     SAAPGTVAPQAPVKPTPSTPAAIPEAVTAPTAKVDSVKAAVAAAPVEAAVPAAVSEPE
                     PEVLAEEPEVIAEEGDSFLKTSKPVYKKWWFWTLIGVAAAGVAGGVAAGVLLYDTGTP
                     MASGTITR"
     gene            16138..>16595
                     /locus_tag="PKH54_00755"
     CDS             16138..>16595
                     /locus_tag="PKH54_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98443.1"
                     /translation="MFEVAASRRKGLAPQFVLLMTTVAAVSCGNPDLGSDHLQLHLVN
                     VSATRTFDGAPQNPESMAAQLMVTVSGPDMETVASDPMDFSQSTFDIPEFPQGLERQF
                     MVEYLNGQQVVARGRSVPLDVVEGGDRVRTQVFVSPLNSITPVTAMDPESG"
BASE COUNT         3081 a         4794 c         5187 g         3533 t
ORIGIN      
        1 agcctggatt atgccggagt ctgtcttttg cagccttgcc gcctcgcccg ggtggggggt
       61 caggatgcag gacgcgccag gcatgtccag ccggccggcc gcaaggatgt tcaacgcgtc
      121 ggcgtcgatt accgcgggaa ggccggcgtc caggacgaac ccgatcatgg attccgtgcc
      181 cggcaacaga ccaagtcccg ggccgagcac aacggcactc ttctggcgga gtaccgagtg
      241 gatggacgcg atttcacagc cgacccacgg gccccgtccc gaatcgccgt cagtcagcct
      301 gaaagccggc tcgaccatca ggtcgggaac cgaaccgtcg attctggatg cggacacgtc
      361 acacgtgacc acggttgcca gtccggcccc cgccctgagc gatgccctgc ctgcaaggat
      421 tgccgcgcca gtcctgccgg gcatgccgcc aatgaccgca acgtggccga aagagttctt
      481 gaacgagtcc agatgtcttg gaagaaatcc cggacatata tcggctggct gaagcagggt
      541 cgcgccgggc gccctttcaa tcagcgcgcg cggaatcccg atcggcgcaa ccgtgatatc
      601 gccgcaatgg ccacgccctg gccagacgta atgtccgatt ctggccgcgc caaaggtcac
      661 ggtcacgtct gcttcaaacg cggttccggc gaccctgccc gtgtccgggt cgatgccgga
      721 ggggatatcg acagccaccg cgggtccctg gagggtgttc aggatgtcga tggtgtcggc
      781 gaagacgcca ccgaccggtc gtaccagtcc cgtgccgaaa atcgcatcga cgaccaccgc
      841 atcggcagcc catgcgacaa ggttgtccgg gattcctgat ggtgcctcga ccacggtcgc
      901 cccgaaccag tcaagtgcct tcaggtttgt aagtgcatcc ccgacgtatc gcgaacgccc
      961 gacaagcagg acgacaacga cttcaagccc ggaatcgatc aggtggcgcg cggccacgaa
     1021 cccgtcgccg ccgttgtttc cagggccaca gaccacgagc acgcgatccg ttccgtgact
     1081 tgcggcgatt gaaaggactg cgtcggcgac ggcccgtccg gccgattcca tcaggacgac
     1141 gccagggatg ccgatcgagt tgattgcagt ttggtcaatc gatcgcattg tctgtgcgtc
     1201 aacaagtttc atcgtatccc cggattccga ccgaccatag gatatattca aggtggtcgc
     1261 ttggcaacgc atccccggcc ggtgatcggg cgccgatgca ccggtttgga tgaaggcagc
     1321 caaacaggtt acaatcgttt ccgacatttt gccgtctcgg gccagaggtg agttgatgaa
     1381 aagacttctt gcgttcggtg ccgccgccgt cgctgcattc gccgccgtcg gagccacagc
     1441 ccaggatgcc ccggcaatca ccgggcagaa ggctgaggcc ggcgcggaag actcccagaa
     1501 gaccggcgtg attctgctga ctccggccga gggacaggtc ttcaccgtcg atgaggatgg
     1561 caggatatac gagaagcggg gctacaaggg cgtggtgccc ggcgttcggg atcaggcgga
     1621 gatccccgga agggcgcctg caccggtggc ctcggccgac gaagaagtgc cgatactgat
     1681 taactggatt ggattccagc cttacgaggc cttttcgcgc gtttttatcc agttatccgg
     1741 ggagttcgag ttttcggtga ctcgtccgga tccgctacgc atcgaggttc ttcttcccgg
     1801 ggcgatgccc ggcacgaaga acgatatgaa cggccttatc accagatggt tccccaccaa
     1861 cgtggatcgt gttgaagtcg ccatggtgcc gtcttccgag accctgccgt ccggcggcat
     1921 tcaggtcggc gtgttcctga agaaggcggt gggcttcctt taccgtcgcg aaggcggcta
     1981 tgtctttgtc gatgtcgaaa cgaaggttga accctgaccc ctttccggtg catgatcccg
     2041 ttgtgcggat aatccatccc gacacgacgg ggctgcagga tgaacagaaa atcctttcgt
     2101 attccttttg cagttgttgc ggtctggctt gtcgtcatga cggcgtcggt ctgccacgcc
     2161 aaggtcggcg tcggtaaact gctctcgaac ctttcgggat cgggtgcggc aaggatcagg
     2221 gcccaggcgg ccctggccct gtcctcgttc gacgacaatc ccgcggttgt gaaggccctc
     2281 gtaagggcgc tcgaggatcc ccatgtgatg gtgcgcagtt cggctgcccg cgcgttgatg
     2341 gcaatcgccc cggccgaggc gttcgcggat atctgccgca tcggtgccca ggaaaaggag
     2401 ccgtttgccc tgaactggat ccagaaggcc gccgcgagat cagccggggc atcgacaaag
     2461 gttctcgtca acgttgacgg tctcgagtgc gtcggggccg ataaccggat gatggtgtcg
     2521 cagaagcttc gaagtgcctt tcttcaaaag cttttcgctt atgacggcta ttccatcggg
     2581 gtttctctgg atttttccgg cgacgcttcc gccgacgagg gcgccggggt ggaactggac
     2641 atagtcggat ccttcaccgt tgacggcaac aagcaggcaa cctccggcaa acttgatgtc
     2701 cgggccgtga cgaccgaagg tttcgtggtc tggtcaaccg tcattgagac tattgatgtg
     2761 gtgcagggaa aggccccgga gataaaggat cccttcgccg atgagttcac catccgcgag
     2821 gaaggcgagg acgcaaggct tgtcgcggcg accgaggccg gaaccagggc ggccaccagg
     2881 cttaatgtcg agctccgagg tgtcgagtag gtttgggccg cgacagtcgc ggcaaacggg
     2941 ggaggccatg tctgataaga gccaggttcc gtgcggttgc caggaaggcg gcaagtacca
     3001 gcgctcggcg gtcttcatcg accgcaggtt ccagaccagg tacctgcttc tgattgcggg
     3061 gacggcactc ctgataatgg tcgtgctcgg cgtgatgtac gccggcgttc ttgccgacca
     3121 gcgcgagttg ctggggataa acgcgatcgc tggcgacact gtcgtttccg gggacgacgc
     3181 cgccctggac gcggcccttt ccagttcgtt ccgttcagag gacgacttgc gcctggccgc
     3241 tcttttcgtc agtgcggcca tacttgtcgg cctgctttcc tgggttgcgg ttcgaatgac
     3301 gttccgcgtt gtcggcccaa tcagggccgc ttcaaacatg ttgcgcggga tccggtgcgg
     3361 cgacgactca tccatccgtc attttcgcaa gggtgatgag ttcatgttcc tttcgcgcga
     3421 cattatcgat ctcagggatt caatccgcga gcgcgaagcg ctgatgaagc ggatgctgga
     3481 tcaggcccgc ctggagatcg agcgcgccgg cggcgacccg gcgattgcag cggagattgg
     3541 ccggttcctt tccagcgatg aagggcggtt tgcggataac aatcgggaag actgatgagg
     3601 tttgtcaggg gattcgaggg gttctgcggt tcagtttcat ccggtaccgt tgtcgcgatg
     3661 ggcaacttcg acggcgttca cctcgggcac gcccaaattt tgtccaacgt ggtcaattcg
     3721 gcccgcgcca gtggcttgaa gtcgtgtttc ctctcatttc atccccatcc gcttcgggtt
     3781 gtcgcaccag agcgggcgcc agggatgttg cagacgatcg aggaaaagct gatatcgctt
     3841 tcggcttacg aacccgattt cgtgcttgtc gtgccgtttt cctgggagtt ctcccagacc
     3901 ccggcgcccg atttcgtcga caagtatctg cttggacttc ttgactgccg ggaacttcac
     3961 gttggggccg atgcgaggtt tggaaggaag cgcgaaggcg acgtttcgat gcttcgcagg
     4021 ctttcgtccg gcgggatgtt ccggctgagc gttaccgaag acgtgcaggt tggcgagcac
     4081 agggtttcgt cctccagaat caggcgatcc attcttgacg gagacatggc tgacgcggtt
     4141 gcgtgccttg ggcgcccctg gacgattgcc ggcgaggtcg ttcacggcgc cgggagaggg
     4201 cgtaagatgg gatttccaac ggccaacatc gcggcggaca actcgatcct tccatcggcc
     4261 ggggtgtatt ccggattttc ccggatcgac tcacggtttc ttccggtcgc cgtccacatc
     4321 gggccgattc ccaccttcgg cgtgaagaac ccggtggtcg aggcccatat cgacggcttc
     4381 gagggcgacc ttgcggggcg ccgacttcgg gtgcaccttg tttccagggt tcgcggaatc
     4441 cggtgtttcg acgggacgcg ggagcttgtt gcaggaattt cggccgacgt cgatgcggta
     4501 cggtcacagg ttctggaatt gcttgacaat agagcttcca tcgcttataa ggttgcccgc
     4561 tttgaccccg aaggaggtga ttgctggtga taccaaaaca gcttgaattc aggccgctgg
     4621 aagttcaggt tcaggacaac gacgttggtc gcgccctgtc cgcgctgaag cgcaagatgg
     4681 ctaacgaggg cctttacaag gaactcaaga agcgccgctt ccacgaaaag ccgagcatct
     4741 ccaagaagcg caagcaccgc gaggccgacc gccgtcgcaa gaagatctcc cccggtggcc
     4801 actgaccgct tttccggtca gccgttctga caattcctgt ttaacaagtc ttgatttcag
     4861 tcgcgcccgc aatcaaggga ctgaagtatt ccgcccgcga ttgcgtaaac ctgttcctga
     4921 agtatccgaa ggcgggcggc gtattccgtc gattccagac cggatcgcgc aaggtcctcg
     4981 cggcccctgg caatctgctc gcgcagcctt atgttctcgt cttccaggag ttgaatttcc
     5041 cgctgcagtt cccgaacagt cttcaacagt tcggcatcct gcccggaatg tcgggcgtcg
     5101 gcggcgtcgc gcccgcccgc atcgtcggtg tcgcgtgccg gaacctggac gcaatccgat
     5161 tccggcggct cgacatcctg ccgatcctcg tcgcagtctg taacttccgg aggccggtcg
     5221 cccgtctgct cctgctcgag tcccagatcc aggttgaact ggcggcacag gcggatgtcc
     5281 tgcaccttcc cgccatgctc ctggattatc ctggtcagcg aaaccctggc gtccttcagt
     5341 gaaactccat caatccgaac caggttcatg atcacggcca tcgtaatgac gtcgttcctt
     5401 gaaaagacgt tctggcccgc catcgaaagc tcgggacgga tatgatcagg gaattccttc
     5461 tgccagtatc tgacggtgga gggagcgacg ccgagaatct gggcaacctc gcccaccttg
     5521 aaatagggcc tttccggtat tggcctttca atcaggaagg ccatactggg cacctccggg
     5581 agcggttacc tgcaggatca cgggttcagg atcgaacgca gaacctggct cggcttgaag
     5641 gtaagaacct tgcgggccgt gatttcaagg ggctttcccg tcttcgggtt gcggcccatg
     5701 cgctcgttct tcgaacggac ggcaaaggag ccgaagctgg aaatcttgac cttctcgccg
     5761 ttgacaaggg cttccttcat cacgtcaaga atggtttcca cgatcgcgct ggcttccttc
     5821 ttggagaatc cgcccagcct ctcgtacacg ccttcgacga tatctgcctt ggtcatgaca
     5881 tttctccgat ttcaattaag ccgtggcacc cacggtctgc ttaacgatct gggtaaaacc
     5941 ggtcgggtcg ctgacggcga tgtccgccag aaccttgcga tccaggtcga ttccgttaac
     6001 cttcatcgca tgcatcaggt cggagtaacg cgtgccgttc atgcggcatg ccgcgttgat
     6061 cctggcaatc cacagcgcgc ggtaattccg tttgttctgc ttgcgatggg cgaacgcgta
     6121 tgccaggctg cgctcaacct gctcaaccgc gtgcctgtac gcagtccttc ttctctcccg
     6181 gtaacccgcc gccatcttca gaaccctgtt gtggcgctgg cgacgcttga aaccgcgttt
     6241 aactcgtgcc atttgacaaa acctccaaat cagtagccgg gcagaaggcg cttaaccttg
     6301 cgaagatcgg ccggggcgac cagggtctcc tggcgcaggt tccgcttacg cttgtgggcc
     6361 ttcttggtaa gaatgtggct cttgaacgcc tttcctcgct taacctggcc ggacgcagtc
     6421 atctggaaac gcttggcggc ggccctgtta ctcttcatct ttggcatctt cgttctccga
     6481 ttccttcccg gcatccttca cgacgccctt ggaagcaaaa tacatcgcaa gaatccggtg
     6541 ttccatccgg ggcgggcgtt ccatgacgga tacttcgacc agatccgccg cgaccctctc
     6601 cattacgtgg taaccgagtt cagcgaactg aacctcgcga cccttgaaaa acatcacgaa
     6661 cttgaccttg ttgccttcca tcaggaaacg cctggcctga ttgatcttgg taacaaggtc
     6721 atgctcatcc gtcttgtaac ggatcttcac ttccttcaga acgacctgaa cctggcgctt
     6781 cttggcctcg gcctgtcgct tcttcttctg gtacttgaat ttgccgtagt ccatgatccg
     6841 gcagaccggc ggcctggaat ccggagccac ttcgacaaga tcaagtccct cttcctcggc
     6901 ctgttcgagg gcctgcgtga tcgacaccac gccaagctgc gagccatcgg atccgatcag
     6961 gcgaacttcc ctggccctga tgcggcggtt gattcgcgct tcgttttcgg cagtcttctg
     7021 ctgttgtccc tgctggatca cgtcccctcg gaaaacttgg gattgacacc acgaaggcca
     7081 cgcccgaaga agccgggccg tgatgccgaa tggtaggcac gagcgggatt gaaccgctga
     7141 catctaccgt gtcaaggtag cgctctccca ctgagctacg cgcctaacgc caaaagcgtt
     7201 ggagatatta cggcaatcgg tggggatgtc aaggggaaaa ttgccggcct tgcaggaata
     7261 atgcaacagt tatcggcgga atctgttgcg cgggggtctg gaattttccg tccgcgcccg
     7321 aatcaatatg gtttgaccgc gcgcttcatt tattggtagt attcggcgcc gaatggcttt
     7381 ttgtcccgta ttgctgtctg gaagtctgtt acgcgccggg cgccgctgcc cggccgggag
     7441 gtcggccgat gttgaaccgg ctagtgctca ggcggcgggt ggtctgtcca ccgctgctgg
     7501 tttgtttcgt ctgctcgatg ttgtggatgc cttttgctgg cttcgcccag tccgccgagg
     7561 gacgggggcc cgccatcgtg ctggtcattc cgccccagaa gacggccgag gttcccgaat
     7621 cggtcgcgtc ggcggtcgag cagaacctga agggactgct cgatatcgat tcaaacatga
     7681 agatgcttta tgacggcgtt cgggatccgg ggccggaata cgatattccc gataccacgc
     7741 cgaaggagcc ggaagtccag gtcaagccgc tgcaagagaa tccgaaggtg gcgaaggccc
     7801 tggattccct ggcaaccgtg cggaagatgg ttgagaagaa gcagtacgaa ggcgcgcttt
     7861 cccggctgct taacctgaat tccacctttg gcgggctggt caaggacatg gacagccttt
     7921 cgcccgtcct ggaagcactg gaactgacgg ccgccagttt catcaacggc gggttcgccg
     7981 aagagggcgc cggggcaatg cgcaacctgc tgacggttca gccggattac gccggcgccg
     8041 gtcagtccga ccgcgtgaag gcggcggttg atcgcaatcg tgaaaggctg gcggcaggcg
     8101 gggacctgat tgtccaggtc aagcccgacg acgcggtcgt ttatgtaaat ggcaggcttg
     8161 tcggccaggg caaccaggtg gttaccggcc tgaagcgcgg gaagcattat gtccgagtta
     8221 cgggagacac gtcgttcccg gacggcggca cggtgacagt gaagcccgaa ggcaatgccg
     8281 tgacggtcag ctatgaactg aagtcccgcc tggttaccat cgccccgcct ccgcccccgc
     8341 cggtcaagca ggggccgaag ccgctggtct ggtacgcccg gaccggcctt tacgcagaac
     8401 ccgccttcga gaacgacatc aaggcggccg cggcgaacgt cttcgcggac aacgtcctgt
     8461 tcgtcttcat cgccaggggc gacaacaagt tccacatcgg cgctttcgtg ggcaacggcc
     8521 tcaacggtgg cgtcgtcgag gttgaaaagg cggttgttga aaccgacctt tccaaccttt
     8581 atatcgagtt gctcggcctt gaaaccagga tccagaaggc cgttgtggat tcatcgtcga
     8641 tggcaaggat tgccgcccag ccgccgattt acaggatggt ccaggcgaag ccgaagccgg
     8701 ttccggtcgt ggtcgtggca ccaccaccgc ccccgccgcc ggcacctgtc gcggttgagc
     8761 cggcgtatca gccttacccg gcaccttatc agcagccggc cccggcagtt gatcccggtc
     8821 ttccggccga tttcccgatg gatcttcctt ctgatttccc gatggccgac accgcccctg
     8881 cggcgcagcc ggcttatcag caggccgctt cgacgaccac ggttcaggaa gaagatgtcg
     8941 tggatgaaga accggtttac aagaagtggt ggctgtggac catcgtcggc gtggttgttg
     9001 ccggtgccgt cggtggcggg ctcgcctggc atttcctcaa ctccgacagc ggtgccagcg
     9061 tcggcggaac ggttggctgg taggaggaat gaaaatgaat aagacaactc tgattgccgt
     9121 cggtctcggc cttgtcgtcc tggctgccgg ttgcggcggc ggcgacaatc cgaacctgtt
     9181 ctccgtccag atcaagaagg ccattgccga aaagacttac gacggggcca atccggatcc
     9241 ggcctccgga gctgtggccg taaggttgac cgtcaccggg ccggggatgg acacccgttc
     9301 gaaggaaagc gacatcggtg tgggtggcag cgccgtaatc ggcattccgg aatttcccga
     9361 aggcgatgac aggcagttca cgtttgagtt tctggggccg gcgaatccat tgaagaacaa
     9421 cgagcgtccc gtcatcggtc gtggccgctc cgcatcgctg actgttgacg ccgacgccgt
     9481 gcgcgccccc gaccggattt atgtggcgcc cgtcaactcg atggttcgcc cgacgaccgt
     9541 tgacgacgaa actggtcttg tcgtttcttc gctgccggtg acggccagcc gtgtcggggc
     9601 gtcgatgacc gaacttgacg acggcaggat tctgatctgc ggcggtgctc aggtgcgggc
     9661 cgaagccgaa acctggtaca tgccggatga cctgaataac cttaccgata cgtgcgagac
     9721 gtatgacccg aagaccggcc tgttcacgga agtcgcgaat cggatgaacg tcaagcgagc
     9781 ctatcaccag gcggtcaagc tcggcagtcc ggacaatccg gatggccgtg tcgtcctgat
     9841 tgggggatac acggttcccg atggcggaac gataacggtc accaacctgg tcgatatctt
     9901 taatccaaag accggcctgt tcatttccgc gacaagcaac gagggtcagc ccgggcttta
     9961 cggtggccag ggcagggcac tgtttactgc cgacctggtc aacgccgaca agaacctgat
    10021 tgtccttttc ggcggctatt ccaatgtcag cgccgtcggt ggcacctatg acatcgtctg
    10081 ggtcaccgag ggctcggtca tgacgatcgc ccacgcacag ctttacaaca aggccgaccc
    10141 ggcgaaggga accgtgcgtt acaaccatgc gcttgcaaag gtcaggaagt acgcggacga
    10201 gttggttgag ggcaatggat accaggcata tctgctgctc ggcggtgaaa acgaatcggg
    10261 cgtgctcgac accgtcgagc cgtacgtcat cacatgtccc ggggtggatg catgcaagct
    10321 gcagcgccag gatcagcttg tcgtgaagat tccgggcggt ggccgttcga tgccggccgt
    10381 cgcgtacgac accctgcaca atttcctgtt tatcgccggc ggatttggtg caaagggcgg
    10441 caagagcccg acggatcgag ttgagatctt ccgtgtgtcc aatgccaact tccgtgaaaa
    10501 tgaggtgctt agcctgggct cgaagatggg tggcatgtcg attgccgaga tggacgacgg
    10561 ccggttcctg cttgccggcg gttgggatgg cacaaaacct gtcgatgcgg tcaagattgt
    10621 ttccccgaag ggcccggtca ttcaaaacgg ccaggaaatc cagacaccgg ccgtcaagtc
    10681 ggccgtcccg accatggatg cggcgcgtgc cggccattcg atgctgacgg atcagaccgg
    10741 ccgcgtcctg atttttggcg gcattgatgc tgaaaaggac agtcccgagc cgattttcta
    10801 caacccgggc gattgattcg gaaccagtgg ccggcggcct ggttctgtcc tgcgatgaac
    10861 cggcctcggt gttgggcgca tgcctatgat ttgttccgcc gccgcacatt tgtccgcacc
    10921 cctcaaagca cttttcaacc ggcagcatgc ggccgttttg gccaaactgg tcttcttaag
    10981 tcttatactt gcccccttcc cgtcttcggc cagatccagg caggttcttt attttccacc
    11041 agcggtttcg ggcccggcgc tttcgacggc ggttcttcaa ctggccaggg aaaggttcga
    11101 gaacgtgctt ggttcgtacg gcatgtatgg cgtggtgccc gacgaagacg cgcttgatcg
    11161 gattggcgcg gccgatgccg gggagttcgg atcctgtcgg gacgcatctt gtcgcacaag
    11221 gcttacaagg cttgcgggcg ccgatgtgag tgcctggttg agcatttccg gccggcgcgg
    11281 ggcctgtgtg gttgaaggcg ttttgagccc ggcatggtct gaacgcaagg atatcaaggc
    11341 caggttcgag ggcggttgcg atgtggtcga aattgttgat gccgtgggcg ttgtggcagc
    11401 catgatgtgc ggcagggatc cccgcgcgac ggcggcgccg gcgaaaccgg aggcggttgt
    11461 tgcgccgggc ccgacgccca ggcttcagcc ccttgagtcc acgaatgcgc ccccaaccgg
    11521 cgtggtcgcc cagccgcagc caccaaaggc attcaaggct gatgaactgg tcggcaggat
    11581 cggctttctt acagtcgaga cgacgccgtc gaatgcatcg atcgagattg atggaaagct
    11641 ggtcggcaag tcgccgttcc aggcggaagt ccaggtcggc aggcatcgca tccgggtgat
    11701 gctggacaaa ttcttccatg aaaagaccct ggaagttgac gtcgggcccg atggacgaag
    11761 ggtcgatgtg cagcttgcgc cggcattcgg caggctggag gtgacctcgg atgtcccggg
    11821 ctcccttgtg accattgacg gcagacgcgt cggaaccacc ccgtatgtcg tggatcgggc
    11881 cctttccggt acttacgtca tcgaagtctc aaacccgagc cgccggccgt cgcagcgcaa
    11941 ggtcgtcgtt tctgacggcc ggactgtcat cgaatcgttc caactggaac aactggtcgg
    12001 cgttctttcc gtcctgacca ggaattataa cgacacgccg tgcagcggac atctgtcaat
    12061 cgacggaaaa cccgcgggtt cgaccccttt caagggaacg gttccgtccg gccgtcacgc
    12121 cctcgaggcg acgtgtgaag gtgtatccgc gaaatcggtg gttgatgtgc cggttgattt
    12181 gcggctgagt gtcgttcttc gaatgccggc ccccattgtt ccggcggctg ccgcgcggcc
    12241 aaccggaatg gagccggtcg ttcgatcgga gcccgcgcgt ccgccggtgg ttcaggacgc
    12301 ccccgcaccg gttgtcaacg tgaagccgga tgacgggatg gtgcaatcgc gggcggcaga
    12361 cgtgatgaat atcgaggatg aacggccgaa aaccgtgtca aagcctcttt acaagaagtg
    12421 gtggttctgg acggtaatcg gtattgcggc cgccggggtc gccggcgggg tggcggccgg
    12481 cgtcatgtcg gccgggggcg ggtcttcgac ggcgtcggga atcatgacgc attgattcga
    12541 acgggggttc tgatgctgga tcgtggctcc tgcggtgtca tgtttcgtgc gtccgacctg
    12601 ttgcgccctg cacttctggc ttgggtggcg gcttgctggc tggcttcctg cggcggcagc
    12661 gagatggcgg cagacaacgt caatctccgt cttgtcagcg tgtctgcaaa gataacctac
    12721 gccgacgcca atccagatcc cgagaacaat gtggtcgcgg ccagggtgtc ggtttcaggt
    12781 ccggacatga gtgacgtttc atccgaattt gatttcagcc tggatgcctt tcagatcccg
    12841 gaatacatgc ttggcatgcg gcgacagttc agcgccgaat tccttgggcc cgcggatcca
    12901 ttgaagaatg acgagcgacc ggttgtctcc cgcggacggt cggttccgat tgatgtcacc
    12961 gaaaggcaaa ccaggattcg ttcccagatt tttgtctcac cggtcaattc attttccagt
    13021 gtcgtcggga tggatgatat ttccggtgaa cccgtcatat cgtatccgat caacgcggac
    13081 cgtgtcgggg cgtcgatgac cgaacttgac gacggtcgca tccttatttg cggcggcgcc
    13141 aaggttcgcg gcccggcggt cacgtggtac atgcccgatg atcttttcac tctgaccagt
    13201 tcctgcgaga cgtacgaccc cgaaacaggc atttttgagg aagttcccga caggatgacc
    13261 gtcaagcgcg cctatcacca ggcggtcaag cttggcagtc cggacaatcc ggatggccgt
    13321 gtggtcctta tcggtggata cacggttccg gatggcggtg ccataacggt ctcccatctg
    13381 gtcgatatct atgatccgag gaccggcctg ttcacttcag cgacaagcaa cgagggtcag
    13441 cccgggcttt acggtggcca gggcagggca cttttcacgg ccgacctggt caacgccgac
    13501 aagaacctga ttgtcctttt cggcggctat tccaatgtca gcgccgtcgg tggcacctat
    13561 gacatcatct gggtcaccga gggctcggta atgacgatcg cccatggaca gctttacaac
    13621 aaggacgacc cggcgaaggg aatcgtccgt tacaaccatg cgcttgccag ggtctggagg
    13681 tacggaagcg aattgacggg cggcaaggaa tacgaggcat atctgctggt cggcggtgaa
    13741 aacgagtcgg gcgccatcga caccgtcgaa ccgtacatca ttacatgtgc tggcgtcgag
    13801 tcgtgcaaac tgcagcgcca ggatcagctt gtcgtgaaaa ttccgggcgg cggccggtcg
    13861 atgccggccg tcgcgtatga cctggcgcac aatttcttgt tcatcgtcgg gggattcggt
    13921 gcgactggcg gcaagaatcc gaccggcagc gttgacgtct tccgtgtgcc cgaggccggc
    13981 ttccgtgaaa gggaatacct gagccttgga atcgatcttg gaggcatgtc gatcgcggag
    14041 atggacgacg gccggttcct tcttgccggc ggctggaacg gcaccgcccc ggtcgatacc
    14101 gtcaagattc tttccccgga tggccccgta atgttcaacg gcctggaagt tgtaagccct
    14161 gacgtcaagt cggatgtgcc ggcaatggat gccgcgcgcg tcggtcattc catgatcacg
    14221 gaccagaccg gccgcgttct gatttttggc ggcgtcgata atgacaggga cagccatgct
    14281 ccgttgatgt acaatccgcg ataaacagtc cgatgttcaa cgtttccgtg gcgacagagg
    14341 tcggttcggt catttcagga aggcatgggg tacgcagcat gagattgaat gaagatggac
    14401 ttttcgggga atccggccgg atccgtggca tgccagggtt gccgtctttt tttctggtga
    14461 tgttgctttc gacagttatc aacctggctc caggaattgc cgcagccagg cagaagatag
    14521 tcctttatta cgcccccacc gcttccggcg tcaggctgtc ggctgcggac cttggtcaga
    14581 tgcgggatca gttcgcaagt gttcttggat ccttcggcct ttatcgggtt gtaagtgaag
    14641 gtgagattcc ggaaagactc gcaccttctg acgtccgtga ttttcggtcc tgttcggatt
    14701 cggaatgcag ggtcaggatt tccaggcttg gttcggccga tgtcagtgcc tggttgaaga
    14761 tttcgggtcg tggcggccgc tgcgtggtcg agggcttcct gagtccggcc tggtcggaca
    14821 agggtgacaa atcggccagt catgaggggg gatgttctgg aagccaggtt gcggcatcgg
    14881 ccgccgtcgt ggcggccctt ctgtgcgaaa aggagcccgg gcaggcagcc atcggaactt
    14941 ccggcggggg tgccgttgtc ccggccgccg gttcggacgg gtcaaccccg gccccagttg
    15001 ccccgccggc acaggttgct gtcgagccgg tcaacaagat ccagatgctt gtccagaaga
    15061 caggtttttt gacgattgaa acaaagcctg tcggtgcgag catcgaaatt gacggccggc
    15121 cggtgggaaa gtcgccattc caggatgaag tggacgtcgg ccgccatcgg atcagggttc
    15181 atctggacga gtattatcgc gatgccacgg cggaagttga tctggaaccc gatggaaaaa
    15241 gtctgacgct tcagcttgct ccggcgttcg gaaagcttga gatcaagagc agggagccag
    15301 gtgcgactgt aacgataaat ggaaatcgtg tcggtaccac tccattcgtc agcgagcgct
    15361 ttctttccgg cacatatgcg gtggaagtct ccaatcccgg tcgtcggccg ctgcggcgcc
    15421 aggttgtcgt ttccgatgga cgaacgacca gcgagacgtt cgatcttgac gtccttatgg
    15481 gcgttctgtc ggttcttaca cgcaataccg atgatacgcc ttgttccgga aagcttttta
    15541 tcgacaaccg ggcggttgga accactccgt tcaaggatac ggtcgcggcc ggcaatcatg
    15601 tgttgaaggc aatatgtggc gatagttcgg caagttccgt cgtggatgtt ccggtcgagt
    15661 cgcgcctgag cgtcgttctg cgtatgggag acaagccgtc ggccgccgtt gccgccgcgg
    15721 tgccggcatc tgctgcgcca ggaacggtgg cacctcaggc acccgtgaag ccgaccccgt
    15781 cgactccggc agcaattccc gaagcggtca cggccccgac ggcgaaggtc gattcggtga
    15841 aggctgcggt cgccgcggct ccggtcgagg cagccgttcc ggccgcggtt tcggaacctg
    15901 agcccgaggt tctggccgaa gagccggaag tcattgcgga agaaggcgac agttttctga
    15961 aaaccagtaa gcccgtttac aagaaatggt ggttctggac cctgatcggt gtcgccgcgg
    16021 ccggtgttgc cggcggcgtg gcagccgggg tgcttcttta cgataccggg acgccgatgg
    16081 cttccggaac cataacccgt tgaatgataa tggagtctgg cgatgttaca ggctgagatg
    16141 tttgaagttg cggcttcccg tcggaaaggg ctggcgccgc agtttgtgct tctgatgacg
    16201 actgttgccg cagtgtcctg tgggaacccg gatcttggtt cggatcatct gcaactgcat
    16261 cttgtcaacg tcagcgcgac ccgcacgttt gatggcgcgc cgcagaatcc cgagtcgatg
    16321 gcggcacaac tgatggtcac tgtttccggg ccggacatgg agaccgtggc ttccgatccg
    16381 atggacttca gtcagtcgac tttcgatatc ccggagtttc cccagggact cgaacgtcag
    16441 ttcatggtcg agtatcttaa cggccagcag gttgtcgccc ggggacgctc cgttccgctt
    16501 gacgttgttg aaggggggga tcgtgtaagg acacaggtgt tcgtttcacc ccttaattcg
    16561 attacccctg tgacggcaat ggatcccgag tccgg
//
LOCUS       DAOPPA010000010        16551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4168_length_16551_cov_3.595720, whole genome shotgun sequence.
ACCESSION   DAOPPA010000010 DAOPPA010000000
VERSION     DAOPPA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4168_length_16551_cov_3.595720"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..>2442)
                     /locus_tag="PKH54_00760"
     CDS             complement(46..>2442)
                     /locus_tag="PKH54_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98444.1"
                     /translation="DIRRIEGGILLGTAGGLLAKGDGESQFSLAWPLPVKTPREPLDK
                     GIRHFSDHDLDGRTVFVTSNLLVTIDVESGEFDQWHVSGADLQAVVVTPSDGQVYIGR
                     GDGLFRLDQAASEGQSEATLELVAGTADIDIRDLAASDTSSQVFAATKDGLLMYEPGK
                     ELKTFTGDNSGLADSNVMSVAMCGSTLAVGSETGFTITGDRSIWTTRTTGIGQLAWGE
                     VIDIACADWGFVLGHEMGATALALDFGASEYFYGLRWMADEVDYEGGSEELHLLGKRL
                     PAVATGPQGEIWLGGPNGLSRIIRVERTLADKEVVFDDMVKHFWRMDGFFSSDGNTAS
                     PWDPMAEMRLHDKDNDGLWTQMMIGGWCFAYAATGDEKYYEYAHRAMENMYKLIDFPW
                     VTFGELGKERGFISRSIISQDSDFIPCSGDFVNNGGSGGCPGPGEPCTVPCIDGKLNY
                     YDYKVCCAEMVTKVDSEGVPHATHLRWNPIEVDGKKYVWKADTSSDEIAGHAFGFPIY
                     YDLCAKDDEERAKVAGYVADLAGYIARNGMNLIDLDGTHTTFGIFGPDTVGIAVDGLD
                     VCMANGHSLELCMGEYYGGAWLNSNEGIGLLLAAWHMTGDKFFYDTYENLITTHKYNK
                     AMIPSEDTLTIVNPSIANHSDHELAMLAYTTVLRYEPNAERLAAWRDGFQFLYDWEKP
                     ERNPWWAGVAALSGMPDPDAENARRTLRELPDDLREWLMDNCHRKDYKINPAKDRHGD
                     RQFTAVPPFDEIRTFWWNGNPYDCSEGGDGRSWNAPTVFLLPYYMAKYTGLITDAPGD
                     "
     assembly_gap    2443..2452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2453..2805)
                     /locus_tag="PKH54_00765"
     CDS             complement(<2453..2805)
                     /locus_tag="PKH54_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98445.1"
                     /translation="MKGNSLFLMVAVTAATVAFMSGAGCGGDATPGQDVLQDSVDPGD
                     IPGDDVDPADVPPTDVGTDTMPDTPPDAADIGLDEIGPDIDQDVPFQYPDAKLINENR
                     NSPVSVPDEPYLQKMT"
     gene            2970..3794
                     /locus_tag="PKH54_00770"
     CDS             2970..3794
                     /locus_tag="PKH54_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98446.1"
                     /translation="MYSLDIDNDPDAKRFEIEKVDVFSDEVGTLLKFRIDPKKAGIPE
                     YVEAWVGVRDSAGRFIKGREPFVDDDGDFLTNAEIVNGNWAVFIPTGALAHDREGVYA
                     VCSVIVSDDPEDADYGLVTGYVSHDVMIPAPRKWDVIEWLRPIIDLCGIMATVSELQT
                     DEEYRMLADNFIDWFEYDGVIDAARLADALKKPVITDMKDAAMAIPLRMPELMPENVV
                     VTLLSLFDVPLENGDKLEGTARAESTFVKKVADLYGIRGNHWKDLLASYQDVQFED"
     assembly_gap    3843..3942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3943..5273)
                     /locus_tag="PKH54_00775"
     CDS             complement(<3943..5273)
                     /locus_tag="PKH54_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOC98447.1"
                     /translation="MKRLTHRIRIFSVVLAAAMSLTSAAASANGFYIQEMTAPGLGQA
                     GAMVAAGEGPANQYWNAANLAYSKGFWFEAGLTTFIPISWYRNDVTGVKTYASDAPQP
                     VPAAFASYRINDWLTVGIAEFTNFGLAINWPKDWEGNHKIISSSMQTFTINPNIAFGP
                     FKGFSIGVGFDAMYGNFKIKKALTLGLDDRPGNPLNLINLSGEAWGFGANIGLMYQPV
                     KWVRIGASYRTAIKIAADSGYVDFDVSEPWAARFKDQRFSAEINLPHIVFLGVRFFPA
                     KGFSLELDAQWIQWSTWDTLKFKLSEGIPLGPTGKQDELSEVSKYHDAWQVRIGGEYR
                     FFKNHFAVRAGFLWDQNPASAQYLSPMLPDSNRVMPCISFGTEWAGFFIDVAYMPVFT
                     LQRTVTWEGDGNNFPGTYKNITHDLTVSLGYHFDVVKGKARIPKYTDREDE"
     gene            complement(5288..7087)
                     /locus_tag="PKH54_00780"
     CDS             complement(5288..7087)
                     /locus_tag="PKH54_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98448.1"
                     /translation="MRHARWFKRLPVPAAAICVLLAAGCLGKPEMTVPEAYDYESRPI
                     LINNADWGVIPLPNNFLNPVKQAEIVYIPGVPLPNAMPTGMALPVVDETAAAVAQALG
                     YPVVEDTALTKSLIAGMNRLNGWVTSFAPKIPFSKIPAYDSIVPFDGTNGDTANLFFL
                     DITDPASPVVIMPGDYYRIINYNRSEAYPYYLSLRLKPGAILQPPQDFTPGHTYAVVV
                     TGMTEETGIRAVVGKDETDADITAPVEVDNTFLVFAARDEECPTEGKCDFKVKHPVTE
                     QEFGLAYIGSQGNPVNSVLEGLDAARQAEGARQITNYVLKIWEGLGGVKDVRDRTEVV
                     AAFQWTTTSNPMPTFFDASAALMGGNPVVASPADYVNGDGELVPVKIKCDPAIGFGFK
                     VAMDTATINQDTVKLFKVTGGQDVTLEKVDVTVTPTVEDGMTNVLVKATAPLSADSKY
                     LVIGTNGMKDAAGNVAVDETYFGLTRTGLAVRNGDGVVTSYIDTPLLTEDRTTWNSPN
                     LDSRLDTMILIHVMNTGDPDPVERPIREVDADLLELAGSGPFGVLGVLKLLDGLRALH
                     KPLFDAVVLQAKAVETREDIVIGWTFTTKATCE"
     gene            7331..10432
                     /locus_tag="PKH54_00785"
     CDS             7331..10432
                     /locus_tag="PKH54_00785"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98449.1"
                     /translation="MVADRERMFRRQSLLTYVGIGAVIAVVSSISPTAGHILLQGWAI
                     ILLVAVYIFATRTFRLTEDRWFLFIGAVLPGVILTRIFEVLSMREFGIFPEFHDGLSI
                     QLRNASRLMEMGVILLAPLLSRAGIHRYLLTASSFGVGIAMVSVLVFSDWIPMCLFEG
                     SQGPCGKILVDIGICAGFLLAAGELLRTVISGHRRKESSIDREKAVLMLVAVAFFLMA
                     AILSAFYLNRYSWIAWADAMLVIAGWYLVLEILTWRGLELPIQMISRSRSQLEAAISA
                     AVDAIVVSDLNGIVIAMNMRFRIFCGLGVEVERDAHVSMFINRAIRSAIKAGHLRQKT
                     GALLESPGAEAFERFEMKDGRIIEMSSVPFMFEGRIDGRVWTFRDVSQRERTTAQLER
                     TSWRLDNILSAAREGIFEMNLDSGALWVHRNWSKVVGCSTGEVPSNWSQWSRMIHPDD
                     LALIQREIRHSGNGSRECGFNFRMASDADGWRWFSSTGRIDLTESSEGGNAWILNGVL
                     RDVTAERARDAEIRRQRSMMEALVQNMPLGAFVKEIPSRRYVIWNSTLSKLSGLIPGQ
                     VVGRRDVEIFGPDVAARIRKADDEVTGQGVSVVLENSWIGDSSGRNVRVRMVPVIDSD
                     RTVTLIIGIVEDVTQQQQIERLAHQSRSMEALGRLAGGIAHDFNNILQVIIGAADSLK
                     VSKAVRGAASEDLDHILDAGDRAMSLIRQLLTFSRQESSTREAVDIGKAASELLKMLQ
                     RLIGADIRVDLDAPVGRYSVFAERSRIEQVIMNLTVNAVDAMPYGGTLAISVTCVDAD
                     DPSASGHPKAASGPWISLAVRDSGIGIPREVLEHVWEPFFSTKEVGKGTGLGLSTVYG
                     VVESLGGFVTVASEMGRGTEFKVFLPQYQPESQRRGEAILPGLEMPEKAGQPVVLLVR
                     ADAAALAADAATLGKAGMKVITASSGPEAMQRLWDADESGGINLMVLDLVISGMGGRD
                     ICDRYLMDHSKTPVLFVQGPSSTVVSMEYLAEVGGRVISSPYSSRQFLLEVRALLGGS
                     VKK"
     gene            complement(11903..13084)
                     /locus_tag="PKH54_00790"
     CDS             complement(11903..13084)
                     /locus_tag="PKH54_00790"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HOC98450.1"
                     /translation="MLALTAAGCGSSGSQGADLAGSDALADDVADASGPDVADAAVHD
                     TTNDSAIDADDDLIPSDVTSDHGDAWVPFYPDTLVMDDVIRLNQIQIKGTHNSYHIES
                     PESLPQWRYTMAPLDVQLESQAVRQVELDIHLTYDNDLEVFHVPFADPLSTCVKFKDC
                     LRIVRNWSDRNPSHLPIFILIEPKDDMDGEDLKLVGRLDLVEQEILTVFKRERIITPD
                     EVRNGHSSLREALVQDGWPTLGQSRGRVMFLMLDNAEHLDAYLLQHPNLEGALIFGRN
                     GRGEPWSSILELMHPDRESRFQEIQSAVDENYLVRSNADDSDTPAAEAEVKAAKAIES
                     GAHLINTDHPVMGSVSNQDPDYKFDLPDGVTAICNAKNAPEGCTPEILERLKPDYGAS
                     N"
     gene            complement(13494..14423)
                     /locus_tag="PKH54_00795"
     CDS             complement(13494..14423)
                     /locus_tag="PKH54_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HOC98451.1"
                     /translation="MTQQEIEELAREFGIDALAAVPSRPVPADRKTLLDLYAGYPPEF
                     AYLARGLEKRLDPGQILAGDRSVWVGLVGYGGLPARPDPLPEGTAWISRFAWCRDYHE
                     TVGARFESLAAALRERHGAAARSYVDTGSILEKSWACRGGLGFIGRNGLLISSALGSF
                     CFIGTIITDIEVPEQQPVAGNGCGSCSACVSACPTGALPGDGRVDVSRCLAHITVTSK
                     EPPPPDINLAGNLFGCDICQDACPHNSPRRPTPVEFLPREGLFCPDLHAVRDACRNRD
                     RFKALFGQTPVLRRKPERLLETVDRLMADPADS"
     gene            14561..15340
                     /locus_tag="PKH54_00800"
     CDS             14561..15340
                     /locus_tag="PKH54_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98452.1"
                     /translation="MDITIDLGECSGAALRTSVVALTHGHADHIGGLAMYLGVRSLYG
                     MPDPTIITPPGTVAAMTDLIDSLGRLQGKPFKARIVPAPEFDRTMDLDNGLRLGTFRV
                     NHYPGDLADGLACGYVLSRVTRRLRSDFVGLPGPVIAEARSRDPGIVTEDVLPLVAVS
                     GDTTPEWIAAAPEHVLQARVLFQECTFLGRGRDPQAAQKGMHTHVDELLKLLPLARNR
                     AVVLYHVSQFYRPEDARELINAAVAGVTGPRVHLFDMGDRL"
     gene            15423..15962
                     /locus_tag="PKH54_00805"
     CDS             15423..15962
                     /locus_tag="PKH54_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOC98453.1"
                     /translation="MTWVRSKTTPEQRRLVKFLMVGGSGVPVNLVTVFIATALMPAAV
                     FSGLRSLLADMFSFPELTSVGVRDIVVYLIGIVVSIFTNFLLNNYWTWGDRVRANPGG
                     GFVSRLAKFYLVSSIAASVQLAVSSVLSPQMRATVIFNFQIWGDYRLYHIAAPSAGIL
                     VGLVINYVANNIWTFRKKD"
     gene            16050..16133
                     /locus_tag="PKH54_00810"
     tRNA            16050..16133
                     /locus_tag="PKH54_00810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16084..16086,aa:Leu,seq:caa)
     gene            16185..>16551
                     /locus_tag="PKH54_00815"
     CDS             16185..>16551
                     /locus_tag="PKH54_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98454.1"
                     /translation="MHLRSTALMALVALSAALAASCGDDSVDPALVWGDPVEEGIYGT
                     MVDIPASTFTQGCDRQNMGCSADEVAKKVEDQVFGFQVSLSAYSIDKTEVTRLAYREC
                     VEDGRCTAPRCNWDPVVNPN"
BASE COUNT         3183 a         4671 c         5030 g         3557 t
ORIGIN      
        1 atgcttcggg acttttcagt tcaagttcgc gtgaacaggg agcggctagt cgccaggggc
       61 atcggtgatc aggcctgtgt actttgccat gtaatacggc agcaggaata cggtcggggc
      121 gttccaggaa cgaccgtcac cgccttcact gcaatcgtat gggttgccgt tccaccagaa
      181 cgtccggatc tcatcgaacg ggggaaccgc cgtgaactgc cgatccccgt gacgatcctt
      241 ggccgggttg attttgtaat ccttgcggtg gcagttgtcc atcagccatt cacgaaggtc
      301 gtccggcagt tcccgcaagg tgcggcgggc gttttcggca tcggggtcgg gcatgcccga
      361 cagggcggcg acacctgccc accaggggtt cctttccggc ttttcccagt cgtacaggaa
      421 ctggaagcca tccctccatg cggcaagcct ttccgcgttc ggttcgtacc gcaggacagt
      481 ggtgtacgcg agcattgcga gttcatggtc tgagtggttc gcaatcgagg ggttgacgat
      541 tgtcagcgtg tcctcggacg gaatcatcgc cttgttgtac ttgtgcgtcg taatcaggtt
      601 ttcgtacgtg tcgtaaaaga acttgtcccc ggtcatgtgc catgccgcca gaagcaggcc
      661 gatgccttcg ttcgaattca gccacgcgcc accgtaatac tcgcccatgc acagttccag
      721 gctgtggccg tttgccatgc agacgtcaag accatcgacc gcgatgccga ccgtatccgg
      781 cccgaagatt ccgaaggtcg tgtgggttcc gtcgaggtcg atcaggttca tgccgttccg
      841 ggctatgtaa ccggccaaat cagcgacata acctgccact ttggcccgtt cctcgtcgtc
      901 ctttgcacac aggtcgtaat agatcgggaa gccgaaagcg tgcccggcga tttcgtcgct
      961 gctcgtgtcg gccttccaga cgtacttctt tccatcaacc tcgatcgggt tccagcgcag
     1021 atgcgtcgcg tggggcacgc cttccgaatc gaccttggtc accatctccg cgcagcagac
     1081 cttgtaatcg taatagttca gcttgccgtc gatgcacggt accgtgcaag gctcgccggg
     1141 gccggggcat cccccggaac caccgttgtt gacgaagtcg ccgctgcaag ggatgaagtc
     1201 cgagtcctgg ctgatgatcg accgggaaat gaacccgcgt tccttgccaa gttccccgaa
     1261 cgtgacccac gggaaatcaa tcagcttgta catgttctcc atggcccggt gcgcatattc
     1321 gtaatacttc tcgtcccccg tcgcggcgta ggcgaagcac cagcctccga tcatcatctg
     1381 ggtccagagt ccgtcgttgt ccttgtcgtg aaggcgcatt tccgccattg gatcccaggg
     1441 ggatgccgtg ttcccgtcac tcgagaagaa gccgtccatt ctccagaagt gcttgaccat
     1501 gtcgtcgaag accacttcct tgtcggccag ggttctttcg acgcgaatta tcctggaaag
     1561 cccgttcggg ccgccaagcc agatttcccc ctggggaccg gtcgccaccg ccggcagtcg
     1621 ttttcccagc aggtgcagtt cttccgagcc gccttcgtaa tcgacttcgt cggccatcca
     1681 gcgcaggcca tagaagtatt cgctggcccc gaaatcaagc gccagggcgg tcgcgcccat
     1741 ctcgtgaccg agcacgaatc cccagtcggc gcaggcgatg tcgatgactt cgccccatgc
     1801 gagttgcccg attcccgtgg ttctggtggt ccagatgctg cgatcgccgg taatcgtgaa
     1861 tccggtctcg gatccgacgg ccagagtcga gccgcacatc gccaccgaca tcacgttgct
     1921 gtccgccagt ccgctgttgt cgccggtaaa ggtcttcagt tcctttccag gttcatacat
     1981 cagaaggccg tccttcgtgg ccgcgaagac ctgggaggaa gtgtcgcttg cggccaggtc
     2041 tcggatgtcg atatcggcag tccccgccac aagttcaagc gtggcctcgc tctggccctc
     2101 ggaagcggcc tggtcaaggc ggaaaagtcc gtcgccgcgg ccgatgtaaa cctgtccgtc
     2161 cgagggcgtg acgaccacag cctgcagatc cgcgccggac acgtgccact ggtcgaactc
     2221 gcccgattca acgtcgatgg ttaccagcaa gttcgatgtg acgaacacgg ttcttccgtc
     2281 aaggtcgtgg tccgagaaat gccggatccc cttgtccaac ggttcgcgcg gggtcttgac
     2341 cggcagcggc cacgccaggc tgaattgcga ttccccgtcc cccttcgcca ggagtccacc
     2401 cgccgttcca agcaaaatcc cgccctcaat cctccggata tcnnnnnnnn nngtcatctt
     2461 ctgaaggtat ggttcgtccg gaacgcttac gggcgaattc ctgttttcgt tgatcagttt
     2521 tgcgtctgga tactggaacg ggacatcctg gtcgatgtcc gggccgattt cgtccagacc
     2581 gatgtctgcc gcgtcgggtg gcgtgtcagg catcgtgtcc gttccgacgt cggtcggcgg
     2641 cacgtcggcc ggatcgacat catcaccggg gatgtcgcca ggatcgaccg aatcctgaag
     2701 cacgtcctgc ccgggcgtcg cgtcgccgcc gcatcccgca ccggacatga acgcaaccgt
     2761 ggccgccgtc actgcaacca taagaaacag gctgttacct ttcattcggg cctccatcaa
     2821 gtcaacggga cactgcccgg aaaccgggcg gaaagtcgat ccgaccggac cattatagtc
     2881 ggcaaatcac aaactttccc cggattgttt gaaagctcgg ggcacttgag gcattatcag
     2941 gccgattctc tttgtgggac gtgcatacaa tgtattcgct tgatatcgat aatgatcccg
     3001 atgcgaagcg tttcgagatt gaaaaggtcg atgtgttttc cgacgaggtc ggaacccttc
     3061 tgaagttccg gattgacccg aaaaaggcgg gaattcccga gtatgtcgag gcatgggtcg
     3121 gcgtgcggga ttcggccgga cgtttcatca aggggcgcga gccattcgtc gatgatgacg
     3181 gggacttcct gacgaacgcc gaaatcgtga acgggaactg ggctgtcttc attccgaccg
     3241 gtgccctggc ccacgaccgc gagggcgtct atgccgtctg ttcggtgatc gtctccgacg
     3301 acccggaaga cgccgactac gggcttgtaa ccggttatgt cagtcacgac gtgatgatcc
     3361 cggcgccccg gaagtgggat gtcatcgaat ggctgcgtcc cataatcgat ctgtgcggga
     3421 taatggccac cgtttccgaa ctccagacgg acgaggagta ccggatgctg gccgacaact
     3481 tcatcgactg gttcgagtac gacggcgtca tcgatgccgc caggcttgcc gatgcgctga
     3541 agaagccggt cattacggac atgaaggacg ccgcgatggc catcccgctg cgcatgcccg
     3601 aacttatgcc cgaaaatgtg gtggtcacgc ttctttccct tttcgacgtg ccgcttgaaa
     3661 acggggacaa gcttgagggc acggccaggg ccgaatcgac attcgtcaag aaggtcgcgg
     3721 atctttacgg tattcgtggc aaccactgga aggatcttct tgcgtcatac caggatgtcc
     3781 agttcgaaga ctgatgaagc cccgcaactt tcccctgaag attttcggta ttgaatcagt
     3841 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcttcatc ttccctgtcg
     3961 gtgtacttcg gaatccgcgc cttgcctttg accacgtcga aatggtatcc cagcgaaaca
     4021 gtcaggtcgt gcgtgatgtt cttgtaagta ccggggaagt tgttgccgtc gccttcccag
     4081 gtgaccgtgc gctgcagcgt gaaaaccggc atgtacgcca cgtcgatgaa gaatccggcc
     4141 cattcggtgc cgaaactgat gcacggcatg acgcggttcg aatccggaag catcgggctc
     4201 aggtactggg cgctggccgg gttctgatcc cacaggaagc cggcgcgaac cgcgaagtgg
     4261 ttcttgaaga accggtattc gccgccgatg cgaacctgcc aggcgtcatg gtacttcgac
     4321 acctcggaaa gttcatcctg ctttccggtc ggtccaagcg ggatgccttc cgacagcttg
     4381 aatttcagtg tatcccaggt tgaccactga atccactggg cgtccagctc aaggctgaaa
     4441 ccctttgccg ggaagaagcg aacccccaaa aagacgatgt ggggcaggtt gatttccgcc
     4501 gagaagcgct ggtccttgaa cctggcggcc cacggttcgg acacgtcgaa atcgacatat
     4561 ccggagtcgg ccgcgatctt gatggccgtc ctgtacgagg cgccaatcct tacccatttg
     4621 acaggctggt acatcaggcc gatgttggcc ccgaatcccc acgcctcgcc tgacaggttg
     4681 atcaggttca acggatttcc aggtcgatcg tcaagcccaa gggtcagggc cttcttgatc
     4741 ttgaaattgc cgtacatcgc gtcgaatccg acgccgatgg aaaatccctt gaacgggccg
     4801 aatgcgatgt tcgggttgat cgtgaacgtc tgcatcgagg aactgatgat cttgtggttg
     4861 ccttcccagt cctttggcca gttgatggca agaccgaagt tggtgaattc ggcgatgccc
     4921 acggtaagcc agtcgttaat ccgataggag gcgaatgcag ccggcacggg ctggggcgcg
     4981 tccgacgcgt acgtcttgac tccggtcaca tcgttacggt accagcttat cggaatgaac
     5041 gtggtaaggc cggcttcgaa ccagaagcct tttgagtatg ccaggttggc cgcgttccag
     5101 tactggttcg cgggcccttc gccggccgcg accatggcgc cagcctgtcc gagcccgggc
     5161 gctgtcattt cctggatgta gaatccgttg gccgaagccg cggccgaagt cagggacatg
     5221 gccgccgcga ggacgaccga gaaaatcctt attctgtgcg tcagtctttt cattcccgga
     5281 gtcctcccta ttcgcatgtg gccttggtgg tgaacgtcca gccaatcacg atgtcttccc
     5341 tggtttcgac tgccttcgcc tgaaggacga cggcgtcaaa cagcggcttg tgaagggcgc
     5401 gcagcccgtc aagcagcttc aggaccccga ggacgccgaa aggcccgctg cctgccagtt
     5461 caagcaggtc ggcatcgact tccctgatcg ggcgctcgac cggatcggga tcgccggtgt
     5521 tcatgacatg gataaggatc atggtgtcca ggcggctgtc caggttcggg ctgttccatg
     5581 tggtcctgtc ctcggtcagc aagggggtgt cgatgtacga agtcacgacg ccgtcgccgt
     5641 tcctgaccgc gagtccggtt ctggtcaggc cgaaatacgt ctcatcgacg gccacgttgc
     5701 cggccgcgtc cttcatcccg ttcgtgccga tcacaagata cttcgaatcg gcggaaagag
     5761 gcgccgtcgc cttgacgaga acgttcgtca tcccgtcctc gacagtcggg gtcacggtca
     5821 catccacttt ttccagcgtg acgtcctgcc cgcccgtgac cttgaacagc ttgaccgtgt
     5881 cctggttgat tgtggccgtg tccatcgcga ccttgaagcc gaagccgatt gccggatcgc
     5941 atttgatctt gaccggcacc agttctccgt cgccgttgac gtagtcggcc ggtgaagcga
     6001 cgacggggtt gccgcccatc agcgcggcgc tggcgtcaaa gaacgtcggc atcgggttcg
     6061 acgtggtcgt ccactggaag gccgcgacca cttcggttcg gtccctgaca tccttcacac
     6121 cgcccaggcc ttcccagatc ttcaggacgt agttggtgat ctggcgggcc ccttcggcct
     6181 gcctggccgc atccagtccc tcaagcacgc tgttgacggg attgccctgt gacccgatat
     6241 aggcgagtcc gaattcctgt tcggtcacgg gatgcttgac cttgaaatca cacttgcctt
     6301 cggtcgggca ttcctcgtca cgcgccgcga acaccaggaa cgtgttatcg acttcgaccg
     6361 gcgcggtgat gtcggcgtcg gtctcatcct tgccgactac ggccctgatg ccggtttcct
     6421 ctgtcattcc agtaacaacc accgcgtagg tgtgaccggg ggtgaagtcc tgcggcggct
     6481 gaaggatagc gccaggcttc aggcgcagcg acagataata aggataggcc tcggaacggt
     6541 tgtagttgat gatcctgtaa taatcgcccg gcattatcac gaccggcgat gccggatccg
     6601 tgatatccag gaagaacagg tttgccgtgt caccgtttgt tccgtcaaac ggaacgattg
     6661 aatcgtaagc cggtatcttc gagaacggga ttttcggcgc gaagctggtg acccagccat
     6721 tgagcctgtt catgcccgca atcagcgatt tggtcagggc agtgtcctcg acaaccgggt
     6781 accccagcgc ctgcgcgacc gcggcggcag tttcatccac cacgggcagc gccatccccg
     6841 tcggcatggc gttcggaaga ggcacgcccg ggatgtaaac gatttcggcc tgcttgaccg
     6901 ggttcaggaa gttgtttgga agcgggatta caccccagtc ggcgttattg atcaggatcg
     6961 gccggctttc gtagtcgtat gcttccggca ccgtcatttc aggcttgccc agacatcctg
     7021 ccgccagcag cacgcatatg gccgccgcgg gcaccgggag tctcttgaac cagcgcgcat
     7081 gtcgcattga aggttcctcc agggaattgt gaatgtgaac cgcacaaagg acgggcacgc
     7141 ttcatcagtg cgcccccgac ccggccggcc cccgccggat ccgctgatgt actgactgtt
     7201 tcctaaacgg attgatgcgg ttcgtcaagg tcggcgtgca aattccggcg cccggtgcta
     7261 aaatcctcct gtataaaaat ttcctggttg cgagcctggt acccgatcca ctcttgaatg
     7321 cggggtgtcg atggttgccg accgcgaacg gatgttccgc aggcagtcgt tgctgaccta
     7381 cgtcggcatc ggtgcggtga ttgccgtcgt aagctcgatc agtccgactg ccggacacat
     7441 ccttcttcag ggttgggcga tcatcctgct ggtcgcggtt tatatattcg ccacgcgcac
     7501 gttcagactg accgaggatc gctggttcct gttcatcggg gccgtgcttc ccggggtaat
     7561 cctcacaagg attttcgaag ttctttcgat gagggaattc gggatcttcc cggagttcca
     7621 cgacggactc tcgattcaat tgcgcaacgc ctcaagattg atggagatgg gtgtcatact
     7681 cctggcaccc cttctgtcga gagccggcat tcacaggtat ctgctcaccg cctcgtcttt
     7741 cggggtcggg atcgcgatgg tttccgtcct cgtgttttcc gactggattc ccatgtgcct
     7801 gtttgaaggc tcccaggggc cgtgcggtaa gatcctcgtt gacattggca tatgtgccgg
     7861 ctttcttctg gcggccggcg aactccttcg aaccgtaatt tccggacacc gtcgcaagga
     7921 atcgagcatt gatcgggaaa aggcggtttt gatgctggtc gccgtcgcat tcttcctgat
     7981 ggcggcgatc ctgtcggcat tctatctgaa ccggtattcc tggattgcct gggccgatgc
     8041 catgctcgta atcgcgggct ggtacctggt gctcgagatc cttacatggc gtgggctgga
     8101 attgccgatc cagatgatca gccgttcccg gagccagctt gaggccgcca tatcggcggc
     8161 ggttgacgcc attgtcgtca gcgatctgaa tggcattgtg atcgcgatga acatgcggtt
     8221 tcggatcttc tgcggccttg gtgtcgaggt cgaacgcgat gcacatgtgt ccatgttcat
     8281 caatcgcgcg atacggagtg cgatcaaggc cggacatctt cgccagaaaa ccggggcgct
     8341 gctggagtcg cccggtgccg aagcattcga gcgtttcgag atgaaggatg gacggattat
     8401 cgagatgtcg tccgtgccgt tcatgttcga agggcgaatc gatggcagag tctggacctt
     8461 cagggacgtc tcgcagcgtg agcggacgac cgcgcagctt gaaagaacca gctggcgcct
     8521 ggataacatc ctgtccgccg cgcgtgaagg catttttgaa atgaatctcg attccggggc
     8581 gttgtgggtt caccgcaact ggtcgaaggt cgtgggctgc tcgaccggtg aggttccatc
     8641 gaactggagc cagtggagcc gaatgatcca ccccgatgac ctggccctga tccagcggga
     8701 gatcaggcac agcggcaacg gttcgaggga gtgcggtttc aatttcagga tggccagcga
     8761 cgccgacggg tggagatggt tcagctcgac cgggaggatc gatctgaccg agtcaagtga
     8821 aggcgggaac gcatggatac ttaacggggt tcttcgcgac gtcaccgcgg aaagggcccg
     8881 agacgccgag atccgccgtc agcgcagcat gatggaggca ctcgttcaga acatgccgct
     8941 tggtgcgttc gtcaaggaaa taccttcaag gcggtacgtg atctggaatt caaccctgtc
     9001 gaagctttcg gggctgattc cgggccaggt cgtcggacgt cgcgacgtgg agatcttcgg
     9061 gccggatgtg gcggcaagaa taaggaaggc cgacgatgag gtgacggggc agggcgtttc
     9121 ggttgtcctg gagaattcat ggattggcga ttccagcggt cgcaatgtca gggttcggat
     9181 ggttccggtc atagacagcg acagaaccgt gacgctgatc ataggaatcg ttgaggacgt
     9241 cacgcagcag cagcagattg aacgccttgc acaccagagc cgcagcatgg aggcgcttgg
     9301 tcgccttgcg ggtggcatcg cgcacgattt caacaacatc cttcaggtca tcatcggggc
     9361 ggcggattcc ctgaaggtgt cgaaggccgt ccggggggcg gcctctgaag atctcgatca
     9421 cattctcgac gccggcgaca gggcgatgtc gcttatccga caactcctta ctttcagtcg
     9481 acaggaaagc agcacgcgtg aggctgtgga tatcggcaag gccgcatcag aactgcttaa
     9541 gatgcttcag cgacttatcg gtgcggatat cagggtcgat ctggacgccc cggttggcag
     9601 gtacagtgtc ttcgccgaaa gaagccggat tgagcaggtg atcatgaacc tgacggtcaa
     9661 tgcggtcgat gccatgccgt acggaggcac cctcgcgata tcggtgactt gcgtggatgc
     9721 ggacgatccc tccgcctcgg gtcacccgaa ggcggcgtcg ggcccatgga tatcgctggc
     9781 ggtaagggac tccggcatcg gtattccaag ggaagtcctt gaacacgtct gggagccctt
     9841 cttttccacc aaggaggttg gaaagggaac cgggctcggc ctgtccaccg tttatggcgt
     9901 tgtcgagagc cttggcggct ttgtcacagt ggccagcgag atggggcggg gaaccgaatt
     9961 caaggtgttc ctgccgcagt accagccgga gagccagagg cgtggcgaag ccattcttcc
    10021 gggcctggag atgccggaga aggccggtca gcccgtcgtt ctgctggttc gtgccgacgc
    10081 ggctgccctg gctgccgatg cggcgaccct cgggaaggct ggaatgaagg ttattacggc
    10141 ctccagcggg cccgaagcga tgcagcggct atgggatgcc gatgaaagcg ggggcatcaa
    10201 tctgatggtt cttgatctgg tgatctccgg catgggcgga agggacatct gtgacagata
    10261 cctcatggat cattccaaga cgccggtgct gttcgtccag gggcccagct caacggtagt
    10321 atcaatggaa taccttgccg aggtaggtgg gcgggtcatc tcatcgccat acagttccag
    10381 gcagtttctt cttgaagttc gtgcacttct gggtggctcg gtcaagaagt gaaaatgttt
    10441 caaaaaaaat tgtaaagaag atcgggccgt cgaagacggg agccgttggc aacccctgtc
    10501 gaaaatgttc gtcagaccca gtcccggtgc tgctttgcgt atgtcgatag ccatgtcgcc
    10561 cgcactggtc gcacattttc cggaattgag cgccgaaatg aggctattgg actgcctgta
    10621 acgcctgttc aaaagttgtg taaaaaattc ggaggtctta tttgtcctgt tttatttatt
    10681 caattaaatc aaggtgtttt tgtgtaattt ggacaccgct attgaccttt ggctggggcc
    10741 gtcctatatt cctgaatgga cgtgacgacg ggaagtcgga tggccggtcc gaaatcctga
    10801 aacgaacccg aaaggggtcg cgaagggaac aaggacaaga accttgaaaa ggccggcagt
    10861 ctcggaagag acaagccaat ttccccggtt ctttgaaact ttgaagtaca gggaaaggta
    10921 cccagaccgg gaaccttcct gaagtacccg tcaggaggcc gaaaggccga ggccggggaa
    10981 ggggaaggcc tcaggaaggg aaaaacccag gagagccagg tccttgaaac ggggggaaac
    11041 cttacgtcgg gacccaacca gcggaaggct taaacccctg aagcgaggcc gtgactcgtc
    11101 attgacgaag tcacgcggcg atcgcggaca ccctgtcgat gaaccggaac caaaggtggc
    11161 aacatgcgaa agcacggcgc ggccggaggc gtaccgacaa aggccggctg cgagagcggc
    11221 gtggatatgt cggggtccgg ggagacaggt tgctgaatcg accttcgggt cgaaaaggcg
    11281 cgtgatccaa ccgtctgcca atcgcaggag gggataccgc ggccgagagg cagcggtgat
    11341 ttccgtttgg gcaagctctg aagggaaccg aaccccatgg acgcgcctgg ttgaaagaca
    11401 ccagggaggt cgcttcggga gcaagacgtc gaaccgcatg gtaagcggaa ggacgtaact
    11461 tgacccgggt ctggatggcc cgggggtggt tgctcgagcg tccgggttgc gtcgaagggg
    11521 cgaaaaacct cgtgagagcg ggcatcgcgc aggcaatgca gggtattcca ggctcaaact
    11581 ctgaagcaga gcgaagtctg aggaaggttt cgcgaacctc gtaacagggg gcgcgccctt
    11641 cagccgaaaa ccgtgaagcg gccagaaccg caggacggga gcgtcgaacc agtaagacgc
    11701 caggctgtcg ggggatcttc gagagacacg ttaactgcaa gagaggatgt cgaacggcag
    11761 cgatgtcgtt gcggcaacct ggaaatattc agctctgggg gggacaacgg tcgtccacct
    11821 gtcttcacgt ctattaaagg gcgtcgtgct tcggtacggc gccctttttt tttctgtcgc
    11881 gtacacaggt acggttgccg gttcaattcg atgcgccgta gtctggcttc agccgttcca
    11941 ggatttcggg agtgcagcct tccggcgcgt ttttcgcatt gcagatcgcc gtgacgccgt
    12001 caggcaggtc gaatttgtag tcaggatcct ggtttgaaac ggatcccatg accgggtggt
    12061 cggtgttgat caggtgagcg ccggattcga tggcctttgc cgccttcact tcggcttcgg
    12121 cggccggggt atccgaatcg tcggcgtttg aacgaaccag atagttttca tcaacggccg
    12181 attgaatttc ctggaacctg gattccctgt cggggtgcat cagttcaaga atcgacgacc
    12241 acggttctcc gcgtccgttg cggccgaata tcagggcacc ctcaagattc gggtgctgga
    12301 gcaggtatgc gtcaaggtgc tctgcgttat caagcatcag gaacatgacc cttccacggg
    12361 attgtcccag cgtcggccag ccgtcctgca ccagggcctc gcgaaggctt gaatgaccgt
    12421 tgcggacttc gtcgggcgtg atgattcgtt cccttttgaa tacggtcagg atttcctgtt
    12481 ccaccagatc caggcgcccg acaagtttca gatcctcgcc gtccatgtcg tccttcggtt
    12541 cgatcaggat gaatatcggc agatgtgaag ggttgcggtc ggaccagttt cgcacaatcc
    12601 gaagacaatc cttgaacttc acacatgttg aaagcgggtc ggcgaagggg acgtggaaga
    12661 cttcaaggtc gttgtcgtat gtaagatgaa tgtccagttc gacctggcgc accgcctggc
    12721 tttccagttg cacgtcaagc ggggccatgg tgtaccgcca ctgcggcagg ctttccggcg
    12781 actcgatgtg atagctgttg tgcgtaccct ttatctgaat ctgattgagc ctgatgacgt
    12841 cgtccatgac gagcgtgtcc ggataaaatg gaacccatgc gtcgccatgg tcggaggtca
    12901 cgtcggacgg tatcaagtcg tcgtcggcat caatcgccga gtcattggtg gtgtcgtgga
    12961 cggcggcgtc agccacatcc ggccccgagg cgtcggcaac gtcgtccgcc agcgcgtcag
    13021 aacctgcaag gtcggccccc tgcgagcccg acgatccgca tccggccgct gtcagggcaa
    13081 gcatcagtaa aagcgacctt ccgaaccggg aattgacagc catattattg aacacccctt
    13141 caggaacctg ggaaaggtaa cggaatcact gggccatatg ctacgtcagc ctgtcgaaat
    13201 cgtgtcaaca attcgctccc gcgcctgtgt gtaatcggtt caaaaagtca atcgcggcat
    13261 cccaggcact tgagtcgacg tttttcgact gaagccgggt cgatcagttg tgcacaagtt
    13321 ttgaacatgt gtgcgaattt aaggggcaag tattcggttt tgccggatat tttcgcggaa
    13381 cgtggctgtc tggaattcgt ccgacgtcag tgcgcgcggc agtccggaag tgttcaaaag
    13441 tgcttccggg gctgtcgaaa tcatcgcttc ttcctgtgca tgaacgccgc ggttcacgag
    13501 tcggccggat cggccatcag ccgatcaacg gtttccagca gtctctcggg ctttcgtctc
    13561 aataccggcg tctggccgaa aagggccttg aagcgatccc ggttccggca tgcgtcccgg
    13621 acggcatgaa ggtcggggca gaaaagtcct tcgcggggaa ggaattcgac cggcgttggc
    13681 cttcgcggcg agttgtgcgg gcaggcgtcc tggcagatgt cgcagccgaa caggttgccg
    13741 gccagattga tgtcgggtgg cggcggttcc tttgaggtca cggtgatgtg ggcaaggcag
    13801 cgcgacacat ccacgcgtcc gtcaccgggc agggcaccgg tcgggcaggc ggaaacacag
    13861 gcggaacatg atccgcaccc attgccggcg acaggttgct gttcgggaac ctcgatatcg
    13921 gtgatgattg ttccaatgaa gcagaatgag ccgagtgctg aagatatcag caggccgttt
    13981 cttccaatga atcccaggcc gccccggcag gcccaggatt tttccagtat agagcccgtg
    14041 tcaacgtatg atcttgcggc cgcgccatgg cgttcgcgga gcgcggctgc cagcgattcg
    14101 aaccttgccc cgaccgtttc atgatagtcc cggcaccagg caaagcggct tatccatgca
    14161 gttccttccg gaagcggatc aggccttgcc ggcagtccgc cgtaaccgac gagtccaacc
    14221 cagacggatc tgtcgccagc gaggatttgc ccgggatcaa ggcgcttttc cagtcccctt
    14281 gccaggtagg cgaattccgg cggatagccg gcatacaggt caaggagcgt cttcctgtcg
    14341 gccggaaccg gccttgaagg caccgcggca agtgcgtcga taccgaattc ccgcgcgagt
    14401 tcctcgattt cctgttgcgt catttcggcg gcgttcccgg attcttccgg tattataggg
    14461 cgtctttgaa cccggtgaag ttctggaaga gcggattgag ggaatccagg tttcaggccg
    14521 gtcgctggcg ggcatttcga ccagcatcac ctttcctgag atggatatca cgattgatct
    14581 cggggaatgc agcggcgccg ccctgcggac gtccgtggtg gcgctgacgc acggccatgc
    14641 cgatcacatc ggcgggctgg cgatgtatct gggggtgcgc agcctttacg gcatgccgga
    14701 tcccacgatc atcactcccc ccggtaccgt ggccgcaatg accgatctga tcgattccct
    14761 gggaaggctc cagggcaagc cgttcaaggc aaggattgtt ccagcccccg agtttgaccg
    14821 gacaatggat ctcgataacg ggctgcgtct tgggacattc cgcgtgaacc actatcccgg
    14881 cgatctggcc gatggtcttg cctgcggata cgtgctttcc cgcgtgacca ggcgcttaag
    14941 aagcgatttc gtcggacttc cggggcccgt gattgccgag gcgaggtcaa gggatccggg
    15001 aatagtaacc gaagatgttc tgccactggt cgcggtcagc ggggacacga ccccggaatg
    15061 gattgcggcg gcgcccgagc atgtactcca ggcaagggtt ctttttcagg agtgcacgtt
    15121 cctgggccgt ggccgggatc cgcaggcggc ccagaagggc atgcacaccc atgtcgatga
    15181 attgctgaaa ctgctgccgc ttgctcgaaa cagggcggtc gtgctttatc atgtcagcca
    15241 gttctaccgt cccgaagatg cgcgggaatt gatcaacgcc gccgtcgccg gcgtgaccgg
    15301 tccacgggtt catttgttcg acatgggtga caggctgtga tagtgaaagt tgaccctggc
    15361 gctggcttca tgtcggccgg ggcgttaatt cagcaagggc gggcgaagtg gctggaagac
    15421 tgatgacctg ggtcagatcg aaaacgaccc cggaacagcg aaggctggtg aagtttctga
    15481 tggttggcgg atccggcgtg ccggtcaacc tggtgacggt attcattgcc acggcgctga
    15541 tgccggccgc ggtcttctcc ggcctgcgaa gcctgctggc ggacatgttc agcttcccgg
    15601 aactgacatc cgtcggagtt cgcgacatcg tcgtgtattt gatcgggata gtcgtctcga
    15661 tcttcaccaa cttccttctg aacaactact ggacctgggg agatcgggtt cgagcgaatc
    15721 cgggcggcgg cttcgtgtcg cgccttgcga agttctatct ggtaagttcg attgccgcgt
    15781 ccgtccagct tgccgtctcg tcggttctgt ctccgcagat gcgcgcgacc gtcattttca
    15841 acttccagat atggggcgat taccggcttt accacatcgc ggccccttcg gccggcatcc
    15901 tggtcggcct tgtgatcaat tacgtcgcca acaacatctg gactttccgc aagaaggatt
    15961 gatggccccg ggcctgcttg acgcaatgac gcattacagc acagttgacc ggcatcgcct
    16021 gtgcgtgtaa tcttgcgctg cattccgttg ccggagtggt gaaacaggca gacgcggcgg
    16081 actcaaaatc cgttgtccgc aaggacgtgt gggttcgacc cccacctccg gcaccttatg
    16141 gccaggcggc ttcatgccgc cttccgcacc ttcggaggat ttctatgcat cttcgatcga
    16201 ctgcccttat ggcacttgtt gcactttctg ccgcgcttgc cgcttcatgc ggcgacgatt
    16261 cggtcgatcc cgcccttgtc tggggcgatc ccgttgagga aggcatctac ggcactatgg
    16321 tcgatattcc tgcctcgacg ttcactcagg ggtgcgacag gcagaacatg ggttgctctg
    16381 ccgatgaagt cgcgaagaag gtcgaggatc aggtcttcgg attccaggtt tccctttcgg
    16441 cctacagcat cgacaagacc gaagtgaccc ggcttgccta ccgtgaatgc gtcgaagacg
    16501 gccggtgcac ggcgccgcgc tgtaactggg atcccgtcgt gaatccgaac c
//
LOCUS       DAOPPA010000011        15145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4656_length_15145_cov_3.900729, whole genome shotgun sequence.
ACCESSION   DAOPPA010000011 DAOPPA010000000
VERSION     DAOPPA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 15145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15145
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_15145_cov_3.900729"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PKH54_00820"
     CDS             complement(<1..817)
                     /locus_tag="PKH54_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98455.1"
                     /translation="MSRELLLSTEADRRVQALYAMLSPCRLCPRRCGVDRLAGEVGFC
                     GTAGAVVSAATAHFGEEPGISGTGGSGTVFFSGCNLRCIYCQNHQISQGVASSTPWSR
                     SDDTPRLAATMLSLQARGCGNINLVTPSHVVPMAVDALVLAARSGLRIPVVWNSSGYD
                     CVEVLRLLEGVVDVWLPDLRYSDGWAASECSSAPDYVDVAHAALLEMARQVGTEPIMG
                     PEGYLLRGMIIRLLVLPNDMAGVRESLDFILEHIGSDARVALMSQYFPSHLAPG"
     gene            complement(814..1506)
                     /locus_tag="PKH54_00825"
     CDS             complement(814..1506)
                     /locus_tag="PKH54_00825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOC98456.1"
                     /translation="MGTRLVLASFNKGKRREVSRILHDIDVLSPPDFGIAGLPPEDGS
                     TFLENAVIKAAFVAFQTRMMAVGDDSGLEVEALEGRPGVHSARFAGPEADDAANIDKL
                     LAELESVGNRKARFACTAVLVVPFQVEPAVLADVQPVQGIKVIQVSAAGRLPDGFSAF
                     AACGTVEGEIIDDRRGHDGFGYDPVFLVPSLGLTFAEIPPDLKNDMSHRGKAFRALAP
                     LIARISDRGWAG"
     gene            complement(1541..4369)
                     /locus_tag="PKH54_00830"
     CDS             complement(1541..4369)
                     /locus_tag="PKH54_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC98457.1"
                     /translation="MPAFHRHRVAVFIACLVASTHAYSADSEYAGMLDTASVGVADFL
                     KGNPVADGRGVVIAILDSGVDVDTPGLKVTSTNENKILVARDFTDEGLVRCGPIDVAP
                     EGTAKTPRGVVNGLKVRDGVKFFAGFLDEVRFRGTAIQDLNRNGRNDDSFAVVVVKAA
                     DGAFQVLVDTVGDWNMSDAVEVGPWEVTFKPLKFIARQAPDINAAVNVGDGPGGTVDV
                     GFHIAAGSHGTHVAGIAAGFGIGGRPGFDGIAPGAKILSLKIGHGGLSGGATVSSSMM
                     RALDFAADWGKRNKAKVVANVSYGVGSVLEGHSAMDSFCRAFSERNPDVLIVASAGNN
                     GPGLSTVGSPAASPGVLTIGAVLSPSIGSEQLGAESMSVPTAYAASSRGGEAAKPDVA
                     APGLAVSTIPGWVRGDTMSGTSMAAPQVAGVAAVVMSGILGQNPQADFHSGMVLRALK
                     ASARPVNGFGPLDVGSGIVDAVGAFKALRKSLSNSMALAVSRLDVETEVPTLGGRKGQ
                     ALFWRASGWAPGRADAATVTVRPVFSRSCGADCRKGFFGRFKVFADSRWIGVSKRGFH
                     VSGDGSASFQSWVEKPEVLGPGVHTGLIRVVSEGGVEAAIPVTVVVPHPDSPVDGVRR
                     ILMRSLRIPKSSVMRIPFDIPDGASSVAIRAVAADGARATASVSLHDGQGNTVHRLSG
                     MIHSEDGRRVEASMSPSGMLEKGTCELVISGGHSIDSVVDLEISFGMISVTGPATFEL
                     KPGKTPVAFIEAVNRMPGAFTGSVKASIDGFETVRSVRLNTGAFEEGFSIGRDFDQVL
                     VDIEFDSRDFAKFTDVSVNVVDRDGERVVQRAMNGRRLAFSVKGGEGHQSDFRLQVTP
                     AFANGGEAVMKVTMTYIWKEQIQLSATLEGAGVIRLYPGIPATLRLEASSTPPAAPSG
                     AAWFGRMDFVDRKDSRSRAMLEFRAVPR"
     gene            complement(4443..5423)
                     /locus_tag="PKH54_00835"
     CDS             complement(4443..5423)
                     /locus_tag="PKH54_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOC98458.1"
                     /translation="MDKRLIPVVILVIASISACTPWRAVKPTDWVRSASDRDDSRCRL
                     AAASSSVISIRNFDDAEYAFQDPDVAVVDFIGGDNECGDVSALQMERFRARSDKSKPA
                     RIVLAWLNVGRAENLRINWFADTPEGKCTPSVMSKIDLEIGDDASIDFTHPGWLVTMA
                     EGDKSYIRRIVDLGFDGVVLGGIDAVRGEDGQVVPEKAQAMFDLVKAISVAAREKDPD
                     FMVVATDPLALSEIEGSMDVLSGVLTTDLIMQEGYFKPEPELTPVLETLNTFRDAGIP
                     VISAEFIIDPIQQTLYQNLCARMGYLCFITNPDFSGPGLAVQAGNGGSCE"
     gene            complement(5500..6597)
                     /locus_tag="PKH54_00840"
     CDS             complement(5500..6597)
                     /locus_tag="PKH54_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98459.1"
                     /translation="MSDDFNKTDETAGAGSKPAGGHEDLKAKLGLRIPERKPAAEQSS
                     APAQDAGKVETAQPVERRAVPVEDIDFDEEPAGKGGVSGGVIGVIIAIALVMAVIGLF
                     VGGVFKGRAIENHKINEAQHLLEYFTTATVAATGGATILDTVRAHIEDTKRVFEAMNK
                     AQSPEEVDKARAELEAYIVRCQKYRDIQPFFTVKNAFYDVLFNSEMANETVVFIDAVR
                     RLYGETVLFALEADTRKQVATLEDTSAAKPSIMFIEPFSKENGERWNKGVWISQLDAE
                     NPIKTASGTDYAMIAMVSGEAFRASTTSLVEFDMAPVALQKSLIHNNAIVARVQGRLG
                     QMIAVINDIDFDSLEAKLKEVAARTPYFTFF"
     gene            complement(6612..8366)
                     /locus_tag="PKH54_00845"
     CDS             complement(6612..8366)
                     /locus_tag="PKH54_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="procyclic acidic repetitive family protein"
                     /protein_id="HOC98460.1"
                     /translation="MNNSVKSALLSIAIILACTSLPALGQEAEPALAPEPAMASEPAL
                     APEPAMASEPALAPEPTLAPEQALAPAAVADSDAGDVPEPDGAKKSESADGALDRLVK
                     SKKIKFEGSYGLHYTYMPTFGLDETGADDGLRHYLDHRLKLRPSIKIRKDLELLIDVD
                     LLAGQLAGSTTDVAESRALYPRSRNDWTGRSMLRELTLTWNSRHGRLQVGQVAAQWGM
                     GLVYNSGRSTDRDWTDNLGSDMIERISFDFAPLAFKWKNSDFARAFRIMLAGDVVFKD
                     QLADLMDEDHAYGFSGAVYFDWNPEKALWRTFTGIHVNYRWQDFHDGFNLRQTTVDFF
                     TEHEFRFRSGDSVKLELEAAVTAGESDRQLHLAPSGFDIAGWGMALRGTYDYHRGVRS
                     SLEFGLASGDGNPMDGKATGFSFDPSYQVGMILFQDVLARSAAWGADRAARHAEDWLN
                     PPGWQMALTDGAVTNALYIHPQFKFYPAKGLDMRLGFLWARALVPVTSYYNLYWLGSS
                     EKEGNPVSFKRAEPSKDLGIELDAGIWYTTPPIWKTLAVRIGVAGGWCLPGEVFSDSK
                     GKGPGNLFKVRVGIDIVI"
     gene            complement(8484..8744)
                     /locus_tag="PKH54_00850"
     CDS             complement(8484..8744)
                     /locus_tag="PKH54_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98461.1"
                     /translation="MQFDWMCPKVNKAFLKKIEGRPNELGRQEIEQRAALLMRLNYPL
                     ARAVRRISDNIAWEYELSARPGFMKDVKEIVECVYRGANRCV"
     gene            complement(9036..10856)
                     /locus_tag="PKH54_00855"
     CDS             complement(9036..10856)
                     /locus_tag="PKH54_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98462.1"
                     /translation="MRKSTSLIACALVLTAGLMTSSCRKDSVPAPGGPELEEVGAIKL
                     PDGVVAFGSVKSLTDITTAATDIGSAFNKGLGPLVGGQVSALVQARLIGTRNLTWMDP
                     TAPMRVVVMDPARFPAPFVFIGTVAYEDGLKEAIEEPSSGVEGNKTAYKGKNGRTMYL
                     NRIGKLAIFSMEPSAFGAVEDFLRSDLLRHPATKLVDCQASAAGFKSVVKPLVDARLA
                     AANTETPTAMDLVASELSDLLEFVDQTSALRLTMSFVDGDLKLEGSALPIEGSTAARF
                     AADAAGRKLVTFRKFMTEGWLEFTSNVNPELFKGLSIKGLESASAFLPFNQQEKDEFE
                     ALVARNLATQSGDMAFQIGYEKQFPFRIAEVVELSDAVAAREAVGKLNAILLAKFGVL
                     VNAALPGAAGGAAPGAVKPATIDFSSVQAAIDTSKEMLGAQGVVASLRTVSADGLDVD
                     SLEFTLDPARLNPADAELARFREVLGDRLSGGIGFDKGAMYLAFGLNSVDDISRMRAS
                     DGKGSTKLARVIEAAGFDVSMAGRVSLVDAAKIAANVAKGVLSRLPSLNTISQRPDLS
                     MAVGSRGNRIVAGYLTIPVAGIAALMQPPSATPNDNPSTP"
     gene            complement(10891..11796)
                     /locus_tag="PKH54_00860"
     CDS             complement(10891..11796)
                     /locus_tag="PKH54_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC98463.1"
                     /translation="MQFAPVPEKLFSPASRVMVILAHQDDEVTFAGLVQRLPPGSRFM
                     WVTNGDGLSDQLGMADADYAEMRRKETVRAMALAGFGEDRLRFLGHGEKSIYARLASL
                     DDAQAITGRAETLSFFKDIAAQVNAEVATFRPEFIITHAWQGGQPEHDLSHLMAVLAA
                     RRIPGCQVFEVPEYELAYTVFLRFGPWRTGPVHEICLSAAEMQVKKAMLACYVSQRRG
                     LFLSRLLAMAGDVLERGRELVLRRRLRMPSFQAREHFAPVPVSRDYRVPPHGIDHLEY
                     IGDDWQKKPMSFRKMVLPVVCALEQ"
     gene            11964..13943
                     /gene="pepF"
                     /locus_tag="PKH54_00865"
     CDS             11964..13943
                     /gene="pepF"
                     /locus_tag="PKH54_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOC98464.1"
                     /translation="MRPEKMIAAAIAATTLSFAVGCSSGKPAEGTGAEQQPATEPVEK
                     EMKTTDKANGGFTPAGNMPRSRIPKEFTWDLSYMYENDEAWEAAFVKASAALKTIEPC
                     RQTITTDPASLLACLEIVFDVKRQLESMGVYAYAFYSTGRESAAHKARLDRIQGLETK
                     FAEASSFVEPMLLSIDPTALKAMVGTLPGLQKYTHWIDDLIRRKPHVLPPEQERLLAL
                     TGDLQATPSFMHGALESDVKFPDTVGEDGKPQSLTMASFPKFRGSRDRNVRREAVAKF
                     FSTLKSYSRSFAASLDIQVKTNILNARARNYGSTVESALDANAVPVDVYMTLLKTTHD
                     NLPRTLHRYVELRRRILGIQEVNYYDLYVPLFPTSDRSVSYEEGVAMTKAALESMGPE
                     YNKVLAEGLDLNNRRVDVYPCEGKRSGAFCNGAWRQPPMVFLNFQNELDDVFTLAHEF
                     GHALHFHLAGASQEFVNADTPIFLAEIASTFNEEMLLDYLLKNASTKEEKLYLLNKRM
                     ENIRTTVFRQVMFAEFELLIHQEVEKGGALTAERLGEIYGGLIREYYGPDFQIGPDDH
                     WEWAYIPHFYYNYYVFQYATGLMSAIALSRAVREGGQAELDRYLKFLGDANSDFPVET
                     LKKAGVDLTKSDAMQATFDLFAATLDQIDALVGQP"
     gene            13956..14135
                     /gene="tatA"
                     /locus_tag="PKH54_00870"
     CDS             13956..14135
                     /gene="tatA"
                     /locus_tag="PKH54_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOC98465.1"
                     /translation="MGIGLKEILLILLVAVLVFGASRIPKALGGLGKGIHAFRKGLKG
                     EEPDRAPDDGDGRGN"
     gene            14152..>15145
                     /locus_tag="PKH54_00875"
     CDS             14152..>15145
                     /locus_tag="PKH54_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC98466.1"
                     /translation="MREKRIGFALVGCGRISRFHVEALRSAEQEATIAAVCDIVPERA
                     RATAGMTEGARAYTDYEAMLRDGGFDCVSICTPSGLHPRHGILAAEHGFHVLTEKPAG
                     IDVLSVDRMIETCSRLRRRLFVMLQNRLNPTVLLMRQALEDGRFGRLFMGQANVFWTR
                     PQAYYDEAPWRGTLEMDGGAFMNQACHYVDLMQWMMGDPEQVTAITGTLGRNIEAEDT
                     GSAVIRFRSGAIGSINVTMLVHPANLEGSFTLMGETGTARLGGVALNTIADWKFDREL
                     PSDMPAASAGYAPPTVYGHGHAGFYRQVIAGLRGGDCTIPDGLEGRRSVRLILAI"
BASE COUNT         3157 a         4804 c         4397 g         2787 t
ORIGIN      
        1 ttcccggggc aaggtgcgac gggaagtact gcgacatcag tgcgacacgg gcatccgacc
       61 cgatgtgttc aaggatgaag tccagtgatt ccctgacccc cgccatgtcg ttcgggagaa
      121 caagaagcct gataatcatt ccacgcagaa gataaccttc cggacccatg atcggttcag
      181 ttccgacctg acgcgccatt tcgagcaggg ccgcgtgcgc cacatcgacg tagtcaggcg
      241 cggaggaaca ctcggacgca gcccacccgt ccgagtacct gagatccgga agccagacat
      301 ccacgacgcc ctcgagcagc cgcagaactt cgacgcagtc gtatccggac gagttccaga
      361 cgaccggtat ccgaagaccc gaccttgccg ccagaaccag cgcatcgacg gccatcggca
      421 ccacgtgcga cggggtgacc agattgatgt tgccgcaacc gcgggcctga agcgagagca
      481 tggtcgcggc cagtcgtggc gtatcatccg acctggacca cggggtggag cttgcgacac
      541 cctggcttat ctgatgattc tggcaataga tgcaccgaag attgcatccc gagaagaaga
      601 ccgttcccga tccgcccgtt cctgaaattc caggctcctc gccgaaatgc gcggtagcgg
      661 cggagaccac ggccccggcg gttccacaga aaccgacttc cccggcaagc ctgtcaactc
      721 cgcatcgcct gggacagagg cggcatgggg aaagcatggc gtacagggct tgaacgcgcc
      781 tgtcggcctc ggttgaaagc agcagttccc gactcatccc gcccaacccc tgtccgaaat
      841 acgggcaatc agcggggcaa gcgcccggaa tgccttgccg cgatgcgaca tgtcgttctt
      901 caggtcgggg ggaatctcgg caaacgtcag gcccagcgac ggaaccagga atactggatc
      961 gtatccgaaa ccatcgtgcc cacgacggtc atcgatgatc tcgccctcga cggtgccgca
     1021 tgccgcgaac gcgctgaacc catcgggcag gcgccctgcc gctgatacct gaataacctt
     1081 gatcccctgc accggctgca cgtccgcgag aacagcgggc tcgacctgga atggaacaac
     1141 caggacggcc gtgcatgcga atcgtgcctt gcggttcccg accgactcaa gttccgcaag
     1201 gagtttgtcg atattcgcgg catcgtcggc ttccggaccg gcaaatcttg cggaatgaac
     1261 gccggggcgt ccctcaagcg cctcaacctc aaggcccgag tcgtcgccga cggccatcat
     1321 gcgcgtctgg aatgccacaa aggcggcttt tataacggca ttctcaagga aggtggagcc
     1381 atcctcgggc ggcagcccgg caatgccgaa gtccggcggc gaaagcacgt cgatatcgtg
     1441 aagtattcgc gacacctcac gccgcttgcc cttgttgaag gatgccagaa ccagccttgt
     1501 gcccaaccgc aaacccctcc ccgccgccgg aagaaccgga tcaacgcgga accgccctga
     1561 attccagcat tgcccttgat cgcgaatcct ttcggtcaac aaaatccatc ctgccgaacc
     1621 aggccgcgcc cgatggcgcc gccggcggcg tcgatgacgc ctcaagacgc aaagttgccg
     1681 gaatcccggg ataaagcctt atgacgcccg ccccttccag cgttgcagag agttgtatct
     1741 gttccttcca gatgtacgtc atcgtgacct tcatcacagc ctcgccccca ttggcgaaag
     1801 cgggcgtgac ctgaagcctg aaatcggact ggtggccttc accgcccttg acggaaaagg
     1861 ccagcctccg accgttcatc gccctttgaa caacccgttc tccgtcgcgg tcaacaacat
     1921 tgacggatac atcggtgaac ttcgcgaagt cacgggaatc gaactcaata tcaaccagaa
     1981 cctggtcaaa gtcgcgccca atcgaaaacc cttcttcgaa ggctcccgtg ttcaaccgca
     2041 cgctccgcac cgtctcaaag ccgtcaatcg aagccttgac cgacccggtg aaagcgccgg
     2101 gcatcctgtt gaccgcctcg atgaaggcaa ccggcgtctt tcctggtttc agttcgaaag
     2161 tggccgggcc ggtaacactg atcatcccga agctgatttc gagatcgact accgagtcaa
     2221 tgctgtgccc acccgaaatg accagttcgc aggtaccctt ttccaacatg ccggacggac
     2281 tcatggaagc ttccacgcgc cggccatctt ccgagtggat catgccggaa agcctgtgta
     2341 ccgtgttacc ctggccgtca tgcaggctga ccgacgccgt tgccctggcg ccgtcggcag
     2401 ccacagcccg tatggcaacg gacgacgctc catcgggaat gtcgaaagga atccgcatca
     2461 ccgaggactt tgggatcctc agcgagcgca tcagaatcct tcttacgcca tcgaccggcg
     2521 aatccgggtg gggaaccaca acggtgaccg gaattgccgc ctcgacgccg ccttcggata
     2581 ccaccctgat caggccggta tgaacgccgg gacccaggac ttccggcttc tcgacccagg
     2641 actgaaagga ggccgatcca tccccggaga catgaaagcc acgcttcgaa acgccaatcc
     2701 acctgctgtc cgcaaaaacc ttgaagcgcc cgaaaaatcc cttgcggcaa tcggccccgc
     2761 acgaccgcga gaacaccggc cgcaccgtca cggtggccgc gtccgccctt cccggagccc
     2821 aacctgaagc cctccagaaa agggcctggc ccttgcgccc ccccagggtc ggcacctctg
     2881 tttcgacatc aagccggctt accgcaagcg ccatgctgtt tgaaagggat ttcctcaaag
     2941 ccttgaacgc gccgacggca tcgacgattc ctgaaccgac atcaagcgga ccgaatccgt
     3001 ttaccggcct tgcggaagcc ttcaacgccc gcagaaccat cccggagtga aagtccgcct
     3061 gcggattctg cccgaggatc cccgacatga cgacggcggc aacgccggcc acctgcggcg
     3121 ccgccatcga agtcccgctc atcgtgtcgc ccctgaccca gcccgggatc gttgacaccg
     3181 caagcccggg tgcggccacg tctggcttgg ccgcttcccc gcccctggag gatgccgcat
     3241 atgcagtcgg aacggacatc gattcggcgc cgagctgctc ggaaccgatc gaaggagaga
     3301 gcacggcccc gatcgtcaga acccctggcg atgcggcagg ggagccgacc gttgaaagac
     3361 cgggcccgtt attgccggcc gaagccacaa tcagaacgtc cggattcctt tccgagaaag
     3421 ccctgcagaa ggaatccatg gccgaatggc cttccaggac tgatccgacg ccataactga
     3481 cgttcgcaac caccttcgcc ttgtttcgct ttccccagtc ggctgcgaaa tcaagcgccc
     3541 gcatcatgct gctgctgacg gtggcgccac cggaaagccc gccgtgacca atcttcagcg
     3601 acagaatctt cgcgcccggc gcgattccgt caaaaccagg acggccgcca atgccgaagc
     3661 ccgccgctat gccggcgacg tgcgtgccgt gagaccccgc cgcgatatgg aatccgacat
     3721 cgaccgtccc gccagggccg tcgccgacat tgacagccgc gttgatgtcc ggggcctgcc
     3781 ttgcgatgaa cttcagcggc ttgaatgtga cctcccatgg accgacctcg accgcgtcgg
     3841 acatgttcca gtcgccgacc gtatcaacaa gtacctggaa ggcgccatcg gcagccttga
     3901 cgaccacgac cgcaaacgaa tcatcgtttc gcccgttgcg gttcagatcc tggattgcgg
     3961 ttcctcgaaa gcgaacctcg tcgaggaatc ccgcaaagaa cttcaccccg tcgcgcacct
     4021 tcaacccgtt aacgaccccc cggggggtct ttgccgtgcc ttccggcgca acatcgatcg
     4081 gaccgcaccg aacaaggccc tcgtcggtga aatcccgggc aaccagaatc ttgttctcgt
     4141 tcgtgctggt gaccttcagg ccgggggtat ccacatcgac gcccgaatca aggatcgcaa
     4201 tcacgacccc cctgccgtcc gccacgggat tgcccttcag gaaatcggcc acgccgacgc
     4261 ttgcggtatc gagcatcccc gcgtactcgg agtccgcgga ataggcatgc gtgctggcga
     4321 caaggcacgc gatgaacacc gctacgcggt ggcgatgaaa ggccggcatg tctgaacccc
     4381 ttcagtattc aggcgggaat tgtatcacgc acgcgcatcc gtcgtgcaag gaaggccgga
     4441 cgtcactcgc aggaaccgcc gtttccggcc tgaacggcaa gtccaggccc gctgaagtcg
     4501 ggattggtga tgaagcacag gtatcccatc ctggcgcaca ggttctggta aagggtctgc
     4561 tggatcgggt caatgatgaa ctccgcagaa attacgggaa tcccggcatc gcggaaggtg
     4621 ttgagcgtct caagaacggg ggtcagctcg ggctcgggct tgaaataccc ttcctgcatt
     4681 atcaagtcgg tcgtgagcac gcccgaaaga acatccatcg agccctcgat ttcggacagc
     4741 gcgagcgggt cggtggcgac aaccatgaaa tccggatcct tctccctggc cgcgaccgaa
     4801 atagccttca ccagatcgaa catcgcctgg gccttttcgg gaacaacctg gccgtcctcg
     4861 ccgcgaaccg cgtcaattcc gccaagaacg acgccgtcga agcccagatc gactatccgc
     4921 ctgatgtagg atttgtcacc ttccgccatg gtcaccagcc aacctggatg ggtgaaatcg
     4981 atggacgcgt cgtccccgat ttcaagatca atcttcgaca tcacggacgg ggtgcacttg
     5041 ccttccgggg tatctgcgaa ccagttgatg cgcaggttct cggccctgcc gacattcagc
     5101 catgcaagaa cgatcctggc cggcttggac ttgtcggaac gggccctgaa cctttccatc
     5161 tgaagcgccg aaacgtcgcc gcactcattg tcgccaccga tgaaatcgac gacggcgaca
     5221 tcggggtcct ggaaggcata ctcggcgtcg tcgaaattcc tgatggaaat caccgaggac
     5281 gaggcagccg ccagcctgca tctggagtcg tccctgtccg aggcggatct tacccagtcg
     5341 gtcggcttga ccgccctcca tggagtacac gcgctgatgg acgcaatgac caggatgaca
     5401 acgggaataa gtctcttatc catgatgcac ctgcctttga gaagcctgaa gccgcgtggc
     5461 acgaaaaccg gactgttcac cgaaatgaag catgccggcc tagaagaatg tgaagtaagg
     5521 cgtgcgggcc gcgacttcct tcaattttgc ctcaagggaa tcgaagtcga tgtcattgat
     5581 gacggcaatc atctgcccaa gccttccctg gactctggca acgatggcgt tgttatggat
     5641 cagggacttc tgcagcgcga caggcgccat gtcgaattca accagcgacg tcgtcgatgc
     5701 cctgaacgct tcaccggaaa ccatcgcaat catcgcatag tctgtgccgg aagcggtctt
     5761 gatcggattt tctgcgtcaa gctgcgaaat ccagacgccc ttgttccacc gctcgccatt
     5821 ctcctttgaa aacggctcga tgaacatgat cgacggcttc gccgcggacg tgtcctcgag
     5881 cgtggcaacc tgcttcctgg tgtccgcctc cagggcaaaa agaacggtct cgccgtaaag
     5941 acgcctgacg gcatcgatga acacgacggt ctcgttggcc atctcgctgt tgaacagaac
     6001 atcgtagaac gcgttcttga ccgtgaagaa aggctgtatg tcccggtact tctggcaacg
     6061 cacgatgtac gcctccagtt cggcgcgtgc cttgtcgacc tcttccgggg actgggcctt
     6121 gttcatggcc tcgaacacgc gcttcgtgtc ctcgatgtgg gccctgacgg tgtccaggat
     6181 tgtcgcgccg ccggtcgcgg ccacggtcgc cgtcgtgaag tattccagca ggtgctgggc
     6241 ttcgttgatc ttgtggttct cgattgcccg ccccttgaaa acgccgccca cgaacagacc
     6301 gatcaccgcc atgaccaggg cgatcgctat gatgacaccg atgacgccac ccgagacgcc
     6361 gcccttgccg gccggctctt cgtcaaaatc gatgtcttcg accggaacgg ccctgcgctc
     6421 gaccggctgg gccgtttcaa ccttcccggc gtcctgtgcc ggcgccgaag actgttccgc
     6481 cgcaggcttc cgctccggaa tgcgaaggcc cagtttggcc ttcaggtcct cgtgtccacc
     6541 cgccggtttt gaacccgcgc ccgcagtttc atcggtcttg ttgaagtcat cgctcatggg
     6601 gccctccact ttcaaatcac aatatcaatg cccaccctga ccttgaagag gtttccgggg
     6661 ccttttccct tggaatcact gaacacttca ccgggcaggc accatccccc ggccacgccg
     6721 atcctgacgg ccagcgtctt ccagatcggg ggggtcgtgt accagatgcc ggcatccagc
     6781 tctattccca gatccttcga tggctcggcg cgcttgaacg aaaccgggtt tccttccttt
     6841 tccgaggaac cgagccagta gagattatag tatgaagtca ccggaactaa agcccttgcc
     6901 cacagaaagc ccagcctcat atccaatccc ttcgcgggat agaacttgaa ctggggatgt
     6961 atatacagtg cgtttgtgac cgccccgtcg gtaagggcca tctgccaccc cggcgggttc
     7021 aaccagtcct cggcgtgtcg ggctgccctg tcggcacccc aggcagccga cctggcaaga
     7081 acgtcctgaa agagaatcat cccgacctga taggaaggat cgaacgagaa cccggtcgcc
     7141 ttaccgtcca tcgggttgcc gtcgccagat gcgagaccga actcgaggct ggatcttacg
     7201 ccccggtggt agtcgtaggt tccgcgcagc gccattcccc atccggcaat gtcgaaaccg
     7261 gatggagcca ggtgaagctg cctatcggat tcccccgcgg tgaccgcggc ctcgagttca
     7321 agcttgacgg aatcgccgga tctgaacctg aattcatgtt cggtgaaaaa atccaccgtt
     7381 gtctggcgca ggttgaatcc gtcgtggaaa tcctgccatc ggtaattgac atgaatgccg
     7441 gtgaacgtgc gccacaatgc cttttcgggg ttccagtcga aatagaccgc tccggaaaac
     7501 ccgtaagcgt gatcctcgtc catcagatcg gcaagctgat ccttgaaaac cacatcaccg
     7561 gccagcatga tgcggaaggc cctggcgaaa tccgagttct tccacttgaa ggccaggggc
     7621 gcgaaatcaa acgaaatccg ctcgatcatg tccgagccca ggttatcggt ccagtccctg
     7681 tcagtcgatc ttcccgagtt gtaaaccagg cccatgcccc actgcgccgc cacctgcccg
     7741 acctgaagcc tgccgtgccg gctgttccag gtgagggtca gttcccgcag catcgaccga
     7801 ccggtccagt cgtttcggga acggggataa agcgccctgg attcggcgac gtcggttgtg
     7861 ctcccggcaa gctgtcccgc aagcaggtcg acgtcaatca ggagttcgag atccttcctg
     7921 atcttgatcg atggacgcag cttcagcctg tgatccagat aatgcctcaa cccgtcgtca
     7981 gcgccggttt cgtccaggcc gaacgtcggc atataggtgt aatgaaggcc gtaggaacct
     8041 tcaaacttga tctttttcga cttgaccagg cgatcgagcg cgccgtcggc gctctcggac
     8101 ttctttgcgc catcgggctc cggcacatcg ccggcatcgg aatctgccac agcggccggg
     8161 gccagggcct gttccggggc cagggtcggt tccggggcta gtgccggttc cgaggccatg
     8221 gccggttccg gggccagggc cggttccgaa gccatggccg gttccggggc cagggccggt
     8281 tccgcctcct gcccgagtgc cggcagggaa gtacaggcca ggattatggc aatggacagc
     8341 aacgcggatt tgacggaatt attcaccggg cctccggttg atgctcgggc gtcggtatta
     8401 taaggtcaaa atgaccggtt ccgcgcatat attccatcac cccggaaacc ggaacagacg
     8461 gtgccgcgca ggacaggaac cgatcagacg caacggttcg cgccgcgata gacgcactcg
     8521 acgatttcct tgacgtcctt catgaagccc ggccttgccg acagttcgta ctcccacgcg
     8581 atgttgtccg aaatccggcg caccgcgcgg gccaacggat aattcaacct catgagaagt
     8641 gcggcacgct gctcgatctc ctgacggcca agttcattcg gccgaccctc gatcttcttc
     8701 agaaacgcct tgttaacctt cgggcacatc cagtcgaact gcatatatcg caagtctcca
     8761 aacgcggtac cgtcggatta tgatgcgccg ggatacatgt gtcaacggcg gcccgtcctg
     8821 cctggcgaaa ggttcggcgt gaacgcgggc gggcgaattt tcattcgccc gtacgggcca
     8881 gcgggggaca ccggcggacg cgatgggcag cattctgctg gatcgcagcg ggcggatgta
     8941 atccgcccgt acagggaggc ggcaggcggg agcacaccga ggcattttgc cggaccggga
     9001 cgggcgattg atcacggagg tgagttttat tgcccctacg gggtggacgg gttgtcgttc
     9061 ggggtggcgg acgggggctg catcaatgcc gcgattccgg cgaccggaat cgtcaggtaa
     9121 ccggccacga tccgattgcc gcgggagcca acggccatgc tcaggtcggg gcgctggctg
     9181 atggtgttca acgacggaag ccgcgacaga acccccttgg ccacgttggc ggcaatcttc
     9241 gccgcatcca caagcgaaac gcggccggcc atcgaaacgt cgaatccggc cgcctcaata
     9301 accctggcaa gcttcgttga accctttcca tcggatgcac gcatcctgct gatgtcatcg
     9361 acggaattca ggccgaatgc aaggtacatc gcgcccttgt caaaaccgat tcctcccgac
     9421 agcctgtcgc ccagaacctc cctgaagcgg gccagttcag catccgccgg attcagccgg
     9481 gccgggtcaa gggtaaattc aagggagtcc acgtcaagcc cgtcggccga gacggttctg
     9541 agacttgcga cgacgccttg tgcgccaagc atttccttag acgtatcaat cgccgcctga
     9601 acggatgaaa aatctatggt cgccggcttg accgcgcctg gtgccgcgcc gcccgccgcg
     9661 cctggaagtg ccgcattcac gagcacgccg aatttcgcca gaagaatcgc gttcagcttg
     9721 ccgaccgctt ccctggccgc cacggcatcg ctcaattcga ccacttccgc gatcctgaac
     9781 ggaaactgct tttcgtaacc aatctggaat gccatgtccc cggactgcgt ggcaagattc
     9841 cgggcaacca gggcctcgaa ttcgtccttc tcctgctgat tgaacggcag gaaggcggag
     9901 gccgattcaa gccccttgat tgaaaggccc ttgaacagtt ccgggttgac attgctggtg
     9961 aactccagcc atccctcggt catgaacttc ctgaaagtca cgagctttct tccagccgcg
    10021 tcggccgcga agcgggcagc cgtcgaacct tcaatcggaa gcgccgagcc ctccagcttc
    10081 aaatcgccat cgacaaagga catggtcagc ctcagcgccg atgtctgatc cacgaattca
    10141 agcagatcgg aaagctccga ggcgacaaga tccatcgccg tcggggtctc cgtgttcgcg
    10201 gcggccagcc ttgcatccac cagcggcttc accactgact tgaatccggc ggcggaagcc
    10261 tggcagtcaa ccagcttcgt tgccggatgc cgaagcagat cactgcgaag gaaatcctcg
    10321 accgcgccaa acgcggacgg ctccatcgag aagatggcca gctttccgat gcggttcagg
    10381 tacatcgtcc ggccgttctt tcccttgtat gcggtcttgt ttccctcgac gccggaagac
    10441 ggctcctcga ttgcttcctt caagccgtct tcgtaagcga ccgtcccgat gaacacgaaa
    10501 ggcgccggga atcgcgcggg atccatcacc acgaccctca tcggtgcggt cggatccatc
    10561 caggtcaggt tcctggttcc gataagcctg gcctgaacaa gggccgaaac ctggccgccc
    10621 accagtggac cgagcccctt gttgaacgcg gagccgatgt cggtggcggc ggtggtaatg
    10681 tcggtaagcg atttcacgct gccgaacgcg actacgccgt ccggaagctt gattgcgcca
    10741 acctcctcaa gttcggggcc gcccggggcg ggcaccgaat ccttcctgca tgacgaggtc
    10801 atcaggccgg cggtcagaac aagggcgcac gcgattaaac tggttgattt ccgcatcgac
    10861 aatcctccaa acgcggttcg cggcatttta tcactgttca agagcacata cgacgggaag
    10921 caccatcttc ctgaaagaca tgggcttttt ctgccagtcg tcgccgatgt attccagatg
    10981 atctatcccg tgcgggggga ctcggtaatc ccttgaaacg ggtaccgggg cgaaatgttc
    11041 ccgcgcctgg aaggaaggca tcctcaggcg ccgcctcagc acaagctcgc gcccgcgctc
    11101 aaggacgtca ccggccatcg ccagcagcct tgaaaggaat agtccgcgcc tctgggatac
    11161 ataacaggca agcatggcct tcttgacctg catctcggcc gccgaaagac atatctcgtg
    11221 aactgggccg gttcgccacg gaccgaagcg caggaacacc gtatatgcca gttcgtactc
    11281 gggaacctcg aaaacctggc agccgggaat ccttcttgcc gcaagcactg ccatcaggtg
    11341 cgaaagatcg tgttccggct gaccgccctg ccatgcgtga gtgatgatga attctggacg
    11401 gaaggtcgcg acctccgcgt tgacctgggc agcaatatcc ttgaagaagg aaagcgtttc
    11461 cgcgcgcccc gtgatggcct gggcatcgtc gagcgacgcc agcctggcgt agatggactt
    11521 ctctccgtgt ccaagaaagc ggaggcggtc ttcgccgaaa ccggcaagcg ccatggctcg
    11581 aacggtctcc ttgcgcctca tctcggcata atccgcatcg gccatgccga gctggtcgga
    11641 aaggccgtcc ccgttcgtaa cccacatgaa cctggagccg ggcggcagcc tctgaaccag
    11701 cccggcgaag gtaacctcgt cgtcctggtg ggccagaatc accatcacgc gcgatgcggg
    11761 cgaaaacagc ttttcgggta cgggggcgaa ctgcatgatg ccggaacctc cagcccgctc
    11821 cggcgggcaa tctccgggac atgccgatgt tgcgacagct tgggcgcgac gtcaaatacc
    11881 gatgagcgcg acacaaaatc aaaaccggcc ggtcgccgac gtgataccat tccgtggcca
    11941 cgcgaagcag aagggaggaa ccgatgcgtc cggaaaagat gattgccgcc gccatcgcgg
    12001 ccacgacact ttcatttgcg gtcggatgct cttccggcaa gcctgccgaa gggacgggcg
    12061 ccgaacagca gccggcaacc gaaccggtgg agaaggaaat gaagacaacg gacaaggcaa
    12121 acggcgggtt cactccggcc ggaaacatgc cgcgcagccg gattccgaag gaattcacgt
    12181 gggatctttc gtacatgtac gaaaacgatg aagcctggga ggcagctttc gtcaaggcat
    12241 ccgcggcgct gaagaccatc gagccgtgcc ggcagacgat taccaccgac ccggcatcac
    12301 tccttgcatg cctcgaaatc gtttttgacg tgaagcgcca gcttgaatcg atgggagtct
    12361 atgcctacgc gttctattcg accggacggg agtcggcggc acacaaggcc aggctggatc
    12421 gaatccaggg tctggaaacg aaattcgccg aggcttcgtc gttcgtcgag ccgatgcttc
    12481 tttcaatcga cccgacggca ttgaaggcaa tggtcgggac gcttccggga ctccagaagt
    12541 acacgcactg gatcgacgac ctgataaggc gcaagcccca cgtgctcccc ccggagcagg
    12601 aaaggctgct cgcgctgacc ggggacctgc aggcgacccc ttcgttcatg cacggcgcgc
    12661 ttgagtcgga cgtgaagttc cccgataccg tcggcgagga cggcaagccc cagtccctga
    12721 caatggccag ctttcccaaa ttcaggggtt ccagggatcg caacgtccgg cgcgaggcgg
    12781 ttgcgaagtt cttctccacg ctgaagtcgt actccaggtc tttcgccgcg tcgctcgaca
    12841 tccaggtcaa gaccaacatt cttaacgcca gggcacgtaa ctacggctcg acggtcgaat
    12901 cggccctgga cgcaaacgcg gtaccggtcg atgtgtatat gaccctgctg aagacgacgc
    12961 acgacaacct tcccaggacg cttcacaggt acgtcgaact gcgccgcagg atccttggaa
    13021 tccaggaagt gaactactac gacctttacg ttccgctttt cccgaccagc gaccgcagcg
    13081 tcagctacga ggaaggggtc gcgatgacga aggccgccct tgaatcgatg gggccggagt
    13141 acaacaaggt tctggccgag ggactcgacc tgaacaacag gcgggttgac gtctatccgt
    13201 gcgaaggcaa gagatccggc gcgttctgca acggcgcctg gcgccagccg ccgatggttt
    13261 tcctgaactt ccagaacgaa cttgacgacg tcttcaccct tgcacatgaa ttcggccacg
    13321 cgcttcactt ccacctggcg ggggccagcc aggagttcgt caacgccgac acgccgattt
    13381 tcctggccga aatcgcgagc accttcaacg aggaaatgct gctcgactac ctcctgaaaa
    13441 acgcttcgac taaggaagag aagctctacc ttctgaacaa gcgcatggag aacatcagga
    13501 cgaccgtatt ccggcaggtc atgttcgccg aattcgaact gctgattcac caggaagtcg
    13561 aaaagggcgg tgccctgacg gcggaaaggc tgggcgagat ctacggcgga ctgatacgcg
    13621 aatactacgg accggacttc cagattggcc cggacgatca ctgggaatgg gcctacatcc
    13681 cgcacttcta ttacaactac tacgtattcc agtacgcgac cggcctgatg tcggcgatcg
    13741 ccctttcgcg ggcagtccgg gaaggcgggc aggcagaact ggatcgctac ctgaagttcc
    13801 tgggcgacgc gaactcggac ttcccggttg aaacccttaa gaaggccggc gtcgacctga
    13861 cgaagagcga cgcgatgcag gccactttcg acctgttcgc cgcgacactg gatcagatcg
    13921 acgcacttgt cggtcagccc tgacaggaga tatccatggg aatcgggctt aaggagattc
    13981 tgctgatcct gctcgtggca gtgctggttt tcggtgcatc cagaatcccg aaggcgcttg
    14041 gcggactggg aaagggaatt cacgccttca ggaagggcct gaaaggcgag gaaccggaca
    14101 gggcgccgga cgacggggac ggacgcggaa actgaaaccg ggggatcgcc gatgcgggaa
    14161 aagcggattg gtttcgcgct ggtcgggtgc ggacgcatat ccaggttcca cgtagaggcc
    14221 cttcggtcgg ccgagcagga agccacgatt gcggctgtgt gcgacatcgt ccccgagagg
    14281 gcccgggcca ccgccggaat gacggagggc gccagggcat acacggatta cgaggcgatg
    14341 ctgagggatg gcggattcga ttgcgtgtcc atatgtactc cctccggcct gcacccgcgg
    14401 cacggaatac ttgccgccga acacggattc cacgtactga ccgagaagcc tgccggaatc
    14461 gacgtccttt cggtcgatcg catgatcgag acttgttcga ggctgcggcg caggcttttc
    14521 gtgatgctgc agaaccgctt gaaccccacc gtcctgctga tgcgacaggc ccttgaagac
    14581 ggccgcttcg gacgcctttt catgggacag gcaaacgtct tctggacaag gccccaggcc
    14641 tattacgacg aggcaccgtg gcgcggcacc ctggagatgg acggtggcgc cttcatgaac
    14701 caggcctgcc actacgtcga tttgatgcag tggatgatgg gggatccgga acaggtgacc
    14761 gcaatcacgg gaaccctggg cagaaacatc gaggccgagg acaccggttc ggccgtcatc
    14821 cgcttcagaa gcggcgccat cgggtcaata aacgtgacca tgctcgtcca tccggccaat
    14881 ctggaaggct ctttcaccct gatgggggaa acgggcacgg ccaggcttgg tggggttgcg
    14941 cttaacacga tagccgactg gaaattcgac cgggagctcc cgtccgacat gccggccgcc
    15001 tcggccggat atgctccgcc gaccgtctat ggccacggac acgccggctt ttacagacag
    15061 gtgatcgccg ggctgcgggg cggggactgt actattccgg acggcctcga ggggcgcagg
    15121 tcggttcgac tgatactggc gatct
//
LOCUS       DAOPPA010000012        14693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4811_length_14693_cov_3.827982, whole genome shotgun sequence.
ACCESSION   DAOPPA010000012 DAOPPA010000000
VERSION     DAOPPA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14693
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_14693_cov_3.827982"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5497
                     /locus_tag="PKH54_00880"
     CDS             <1..5497
                     /locus_tag="PKH54_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOC98467.1"
                     /translation="APAATFFILALSPIFLGAGCGGDGGIKDARFVPCEGPAPSSSNA
                     GFIHGKVLAAENDEPLGGAAIRIDGVEGCVSTESDGTFTFPLKDGGKFGLTATAAGRT
                     HARRVMAVTAGHDMNVGMMFLPRRDAAISRIGPDGGVHKSAGGGLELRFPAGALDQVR
                     EVSATRFGAGRELPGRLPETSHFTMALEAVAGEGDLNAPVTITIPNDYGFPPGTNVPV
                     GVFDEATGEWVPDGMGVISEDGTQVVYEARHFSAFDCNYPVETKADPGLNGKDPRRER
                     DPCNRNSTSGNSIVDIRSGHLRLDFDMPVERYAGLERTVGLVFQSGAATPALWTGGAW
                     EDGGTNPEDTEYAAIEVQVEGLLEKVYVQVAEKDNWAAHVWDGRNARGEVLPTGLYDG
                     WIKVFNGRKGIFAQADVFGGQPTYSLGIEADEMVEADTVITTRYQLINGRDSSVGNGW
                     YLKDIPELFLHPDGAVMVVMNGESGGIFLPDVKAVRWAGAGQESDCGDGAPRQNSCLE
                     YPEDMVVAPDGSVLTTDDWQRRIVRIKTDGTTETVFADSGNEYDIVSIAVSPSGITYF
                     ADGYSGKVFRLSNGSPEFVVGGDFPDIFGSAPSVSSPTDLDFDAEGNLFIADWPLGLR
                     KLDPNGVLSNAYEGDNPDWPAPVAIDVTDDGSILLAEPMRHRVRRLTADGRIEPVAGI
                     DGEQDFRGDGYLAVKARLDTPSAVVQGPQGTIYITDEGNDRIRAVTPDGIIRTFAGKG
                     SVSGAGWGDGGPATDTLLTEPDSLAIDADGNVLLLDHMNGWIWKYDLSTMVYSRPPSQ
                     SDVLSRVDADGDFVLRHDDTDFAFDAAGQLTSMVGPDSDKATFTRDEAGRITDVSWQS
                     GSKLTFEWSAEGLRAIHAPGSRTLTVESDADGNITGLTMPDGAKTALSYDDDGRLNGW
                     TDAEGRTAEYGLDTYGRVASVKAADGSVRTYRPVETTALINDLIREGAGVSPEDPAPA
                     IVMPAAEYTDGSGKTWKYKYDALGEAVEIENPDGLVVQMGNHSCGMPSMFSIPGDYAW
                     TAFYDEQGRILVQNGPQGETSYRWDEESGELESITTPAGRGVSFEYDDDARMTAITVD
                     GIRIHSYAYESGLRLKSIEDAIGRTTEYRYDDKGNLFKVIQSSIPVATFERNEIGEIT
                     AITDAIGGRTEFGLDPMGRVVSVKDRAGAFHSIGLDAMGQVISLKSAGQGLDDEKMTT
                     WSRDGKGRIQSVSLLGAPEWTINSDGEDRVMSIASPGGSVAVTYDGMGRVVSRTIVTT
                     NQAVPDTTAIFEYDADGHLVRAVDDDSEITWEYEPRYGNLLSVTQFYQGMAEPITIGY
                     DQDEDGWINAVEYPALDGFDGGRFDYYYHEPSQRVEEITAPDGMYFDLYRDDAGRLET
                     FEIDWGDPFLLEAAYDETGYLASITAYLDSYREDVHMNFEYVTDDDGAIMSETGPSGT
                     TAFGYDQLRRVTGATNQAAGRDETFDYNARGDRMGAGFEYDDFGRAIAGAGFTFEFDE
                     AGRVTRRVETATGNAVEFLYDGDDNLVRASVYSGGSMTPEHVVTYGYDPLGKRIFRDV
                     DGVRTFFIHDFTDVVLEINETGGADAFYLHEPAMDRPIAMYRDGEVYACIQDGFGSIC
                     GLVRLSDRSLVWTREYGVFGDILDQTGTIPFRYGFHGREHDPLTGLIHFRAREFDPAV
                     GRFVSTDPEKFTSFAGTYAFPGNDPVNRHDPTGAGPRAVRAAGEVYSYYNKTKQHIEQ
                     SIDSRVSSVAGRDAGAASSFAWKTAGAGMLDSGVKILLPSPYQSPGPSVTDAAVWAYK
                     AYKAKDPCARAAVGGDVLVDIMPAGDKWLRPMVDKFNNFGNLMNSGN"
     gene            complement(5502..6905)
                     /locus_tag="PKH54_00885"
     CDS             complement(5502..6905)
                     /locus_tag="PKH54_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98468.1"
                     /translation="MKTADVATRLSVATLFVVLCGCGDSTAGSPDSGNDAGGEQWKLE
                     WVGDGMPGTVAASGQLELSTQVTLGDVAQSGVTVSFEIVFGGGMISQAAVVTDASGIA
                     STTWTAGPISGRAVLRASIDQAAVFIDHHVDVAAVQPLVPQPFGDVGALLEGAGIDGS
                     TEGLVFDDAGRLFLAVPGNLVAMRPDGTAQLVPVDGDGLTSPLGMAWDYLRKVIWVAD
                     AGSVRKVSISEVGGTITATATTMAGFDGLVTPNSVAIADNRYVYFTDSCTGGVYRFQP
                     DADTVVLETVAMFDVTVRGGPNGLAFDGSGLLWVTTENVGLLCPDSGADVTLNHGGLY
                     SIDLSGGLAGGPVQPTPRLEGFAMFGDGLAFDADGTMYVIFDMFDGFTLTDSSVWVVP
                     ASADEPPYKVISAPVPDGATSPDRLIANIAWGYDEFGSSRLYMSLLAVVLVVSERGAM
                     VVDVGIGGQSLLPADLP"
     gene            7057..7386
                     /locus_tag="PKH54_00890"
     CDS             7057..7386
                     /locus_tag="PKH54_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98469.1"
                     /translation="MCSTGIFEIIMLLCFSAAWPFSIARSFRSRSTSGKSLVFLLVIL
                     AGYAAGIVHKANCNRDPVISLYIFNGSLVLIDILLYLRNRIVERRASVDSSQVHGRTE
                     NTRGEIE"
     gene            7383..8498
                     /locus_tag="PKH54_00895"
     CDS             7383..8498
                     /locus_tag="PKH54_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98470.1"
                     /translation="MKAKITILVLAGMFLLVAGCGGDNISTNDVLQDIFIPFDTNQPD
                     LVVNPDADDRDQNVPVDHGTGTDTNVPTDTNVPTDTNVPTDTNVPTDTNVPTDTNVPT
                     DTNVPTDTNVPTDTGNQTDTVDVEGCTGSCTYGTDAMWCSEDGLSVCFCGEDGEWMAF
                     NCADACANSNMVGSQCINDDGAEYCSCEFDCTRTDLTTSQCNAYSYTPCTCAAADPCN
                     WDNDGWCDKICELEFPEDSFTEPNDCDCGGECDPDEGIAYCDNDQGLCWCYEGYFESL
                     PCTDFCDYLGAELDPEQWCFVDMSEQYQCSCVNYSCVDSDAVQNQCDFGVYSECTCAA
                     ADPCGWANDQYCDTPTCQYLYPELSVFDDTGSAACGT"
     gene            complement(8529..10583)
                     /locus_tag="PKH54_00900"
     CDS             complement(8529..10583)
                     /locus_tag="PKH54_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC98471.1"
                     /translation="MTKGLSWLDALGWRPGFMIDARVAEMYRRAVENDLISPDDALAC
                     RDILAIAGWRVADAEADFAVNCLMAGLFSVCNQGSLCLPLDVGTLETEFGRLLATTGD
                     VVDSLPSAGHVAARAASLIDDGLPLFANPDGWDPYPLVTDGRFLWFSRHHVHETGLNA
                     ALQRLALTPGGQFVSSPLSGMDLESVVSQVGAGREGRDLNERQKSAVMKALVKRFVVV
                     SGGPGTGKTRILAALLRAWCLCGAVPGEMLLVAPTGRAGRRMGESISAAIDDAIIGPD
                     EFQVEDIRGLTGSTIHRALGFRPSWNDFRFGSDNPLPHRYVVCDEVSMVDAALMNRLL
                     QAVRPDARVVFLGDRDQLPSVDSGTVLADIIPRGDVSGPMKDSIVMLSQSYRAGGQVL
                     SLAGVANAGDGQRFLDRAGRLADRVPDRDEWGLPGAACVILRPDYPRQGGIGPMLESW
                     VSWIYGCRPEAGSIGRAFEALAARRLDTRFAPGSEDAVVMNGLFRALQMGRILCVLRD
                     GPEGVVGINRAIARLAGGRDGLFAGGRDGLFAGCPVLVTRNIAAQGLFNGDVGMVMND
                     LDGQPVAIFDDGVVFRSFPVQSIPGLEPAFATTVHKSQGSEYGNVLLFLPSRADHPLL
                     VREILYTAMTRAKNNVWIHGTDESIRGAISRKVSRYSGISPWNRPNPPHDPIHTMPRT
                     GE"
     gene            complement(10580..13939)
                     /locus_tag="PKH54_00905"
     CDS             complement(10580..13939)
                     /locus_tag="PKH54_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC98472.1"
                     /translation="MSPLTSSTGVTMDLSGVIDRHAVIQASAGTGKTYTLEHIVLQIL
                     LDFPDVSLRNILLVTYTEKAAGELKQRIRKRLMDTAGGKDVSPATAARLRIELDDFES
                     ASISTIHAFCNRVLMEFAFENSRPLELQQGGDSDMRSRLLNRIVRDEWPRIDPRRMNA
                     KGTLPIASEALGRLREVLNVAGEKRSLDGLLPEFDGDLADAVSTAQILAWTTSALCAA
                     VAREKMASSLVTFGDMISDVRSALTMPGPAGRALLERLRKRFLFGIVDEFQDTDPDQW
                     DIFRTVFVEGGGPNRLIVVGDPKQAIYGFRGADLRTYLSACSEIERRGGNILSLQTNY
                     RSSVPMIEGFNGIFRDTGWFGTDIECPELLAPADGRPQDLVADASGRAAVVPVHIMRP
                     GEKGKLEVDEARYRTARFIAKEISRLTGSGGIILPGRAGQGPRPLGLDDICILIQKND
                     DFRLISRLLRAEGIPHAQYKQKKLYESAEAENVLVLLKAISRLGAGPGSQSIDSVLLT
                     RFFLDRPDPADMLEPERRNHAARTLEAWRALAIDRNWAMFFEAISRDTALFVREAAEF
                     DGERRLANFRQIFGELASFADSGSLDLHGLIGRMEQLKAGSGDEEESTMARLESEQPR
                     VRVLTIHAAKGLEFPIVFVAAGFSGPRSGKSCRGYIGRDRYFSFERPYPGLVEDNDLP
                     RLFYVALTRAMYKLYVPTDWTPSKNDGAPICTFIKAGLARLAQLTESGGRVRSDVDCG
                     GIPIGSVAPEIVCVDDSGKRQEGSASPHPMLPDLTPGAQIRWPLRRLWVDSYSSLTGL
                     AGRAQFDVEGMAGSRADDATDDEAIEPMIGAPTTADAAGIQATPLLMAGRHTGDAFHQ
                     LMQDIACGRAGDDGNPLDFSDFARLDSPAAIMASNVGSLIIQKMKSNGVPLKFGQPGP
                     DGSVDSSELQFASLVWSALRGHLGDSAPLDFSISDLSSSDTLAEVEFHLDESRFGSGP
                     LAPGNMADGIPPWRSGLLNGVIDLVFQKDGRFWFLDWKTTSVRDEGGRNPDWSAGAIS
                     RAMRDNGYIVQAHVYRIAMLQWLESVFGPGSGHLLAGGLFLFVRGMGPPGRGVWNSRE
                     IFTDTDGSESRLFVRKLLARSAAHAAGRGRE"
     gene            complement(13936..>14693)
                     /locus_tag="PKH54_00910"
     CDS             complement(13936..>14693)
                     /locus_tag="PKH54_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98473.1"
                     /translation="YFRQLSELGVRKALSNTPEAIMDSIGGMKPLSEAVMGQVSFSRG
                     APGLRLAPASFSARISKPDGSSIDMAVEVTGRVDFVVPDLDGLPLIIGNVSASGASET
                     GPFPRALIMPTLFGIVAGIGAGRREIDFGAVVVCADKNVARRIKVPVSTAAGWIEDLI
                     SDYLDCRRILENLPYDVAVKYAGLSPDEPEDEDFRRAMRARIEKGSQGGWATQPINAL
                     QKLVWDRTVLPERIRETIESRYGLLWRCGGGGA"
BASE COUNT         3182 a         4553 c         4316 g         2642 t
ORIGIN      
        1 ggctcctgcc gcgactttct tcatcctggc tttgtctccg atcttccttg gcgccggttg
       61 cggcggcgat ggcgggatca aggacgccag attcgttcct tgcgaaggac cggcaccatc
      121 ttcaagcaac gccggattca ttcatggaaa ggtcctggcc gctgaaaacg atgaaccgct
      181 gggtggagcc gccatcagga tcgacggcgt tgaaggctgc gtttcaacag aatcggacgg
      241 gactttcaca tttccgttga aggatggcgg caagttcgga cttaccgcca cggcggccgg
      301 acgcacgcat gcccgccggg tgatggcggt gaccgcgggc cacgacatga acgtcggcat
      361 gatgttcctg ccaagacgcg acgcggcgat atcaagaatc ggacccgacg gcggcgtgca
      421 caaatcggcg ggcggaggcc tggaactcag gttccccgca ggcgccctgg atcaggttcg
      481 cgaagtaagc gcgaccaggt tcggggccgg acgggagctt cccgggcggc tgccggaaac
      541 atcgcacttc acaatggcac tggaggccgt cgccggcgaa ggcgacctga acgcccctgt
      601 cacgataaca attccaaatg attacggatt ccccccgggg acaaacgtcc cggtcggcgt
      661 gttcgacgaa gcaaccggcg aatgggtgcc ggacggcatg ggcgttatca gcgaagacgg
      721 aacccaggtg gtgtacgaag ccaggcactt cagcgcgttc gactgcaact accccgtcga
      781 gacgaaagcc gacccgggcc tgaacggaaa ggatccacgc cgcgagcggg atccgtgcaa
      841 caggaactcg acttccggca acagtatcgt tgacatcaga tccggccacc ttcggcttga
      901 cttcgacatg cctgtcgaac gttacgccgg ccttgaacgc actgtcgggc tggtattcca
      961 gtccggtgcg gccacccctg ccctgtggac gggcggggca tgggaggacg gcggcacaaa
     1021 tccggaagat accgagtacg ccgccatcga agtccaggtc gaaggtcttc tggaaaaggt
     1081 atatgtccag gtggccgaaa aggacaactg ggcggctcac gtctgggacg ggcgcaacgc
     1141 ccgcggtgaa gtattgccga ccgggcttta cgacggctgg atcaaggtct tcaacggacg
     1201 aaagggcatc ttcgcccagg cggacgtttt tggtggccag cccacgtaca gcctggggat
     1261 cgaggcggac gaaatggttg aggccgatac cgtcatcaca acccggtatc aattgatcaa
     1321 cggccgtgac tcatcggttg gaaacggatg gtaccttaag gacatcccgg aactcttcct
     1381 gcacccggac ggtgcggtca tggtcgtcat gaacggggaa tcgggcggaa ttttcctgcc
     1441 ggacgtaaag gccgttcgct gggcgggcgc cggtcaggaa agcgattgcg gcgacggcgc
     1501 gcccagacag aacagttgcc ttgagtaccc ggaggacatg gttgtcgccc ctgacggaag
     1561 cgtcctgacg acggacgact ggcaacgtcg aatcgtacga atcaagacgg acggcactac
     1621 cgagactgtt tttgccgatt cgggaaatga atacgacatc gtgtccattg ccgtgtcccc
     1681 ttccggcata acgtacttcg cggacggata cagtggaaag gttttcaggc tgtcaaacgg
     1741 cagtcccgaa ttcgtggtcg gtggtgattt cccggatatc ttcggcagcg ccccatcggt
     1801 gtcgtcgcca accgacctgg atttcgacgc cgaaggaaac ctgttcatcg ccgactggcc
     1861 gctgggactt cgcaaactcg atccgaacgg cgtcctttcg aacgcctacg aaggcgataa
     1921 cccggactgg ccggcgccgg ttgcgatcga cgtgaccgac gacgggtcca tactgcttgc
     1981 agaaccgatg aggcatcgag ttcgacgcct tacagccgac ggcaggatcg agccggttgc
     2041 gggaatcgac ggggaacagg atttccgggg cgacggctac cttgcggtca aggcaaggct
     2101 tgacacgcct tcggccgttg ttcagggccc acagggcacc atttacatca cggatgaagg
     2161 caacgaccgc atccgggccg tgacgccgga cgggattatc cggacattcg cgggaaaggg
     2221 aagcgtttcg ggagccggat ggggcgacgg cggccccgcc accgatacgt tgctgaccga
     2281 accggactca ctggcgatcg atgctgacgg aaacgtcctg ctacttgacc acatgaacgg
     2341 ctggatatgg aagtacgacc tttccacgat ggtctattcc aggccgccaa gccagtcgga
     2401 tgtcctttcc cgggttgatg cggacggtga cttcgttctt cgacacgacg acaccgactt
     2461 cgcctttgat gccgccggac aactgacttc gatggtcggc cccgactccg acaaggcgac
     2521 attcacgcgg gatgaggcgg gcaggataac cgatgtttcg tggcaatccg gttcgaaact
     2581 gactttcgaa tggtccgccg aaggtcttcg ggccatccat gctcccggct cccggacact
     2641 gacggtcgaa tccgacgcgg acggcaatat caccggattg acgatgccgg atggggcgaa
     2701 gaccgcattg tcgtacgacg acgacggccg actgaacgga tggactgacg ccgaaggccg
     2761 gacggccgag tacggactgg acacttacgg ccgggtcgcc tcggtcaaag cggctgacgg
     2821 ttcggtcagg acgtaccggc cagttgaaac gacggcgttg attaacgacc tgatccgcga
     2881 aggcgccggt gtatcccccg aagacccggc cccggcgatt gtgatgccgg cggccgaata
     2941 taccgatgga tcgggaaaga cctggaaata caagtacgac gcgctcgggg aagctgttga
     3001 aatcgaaaac cccgacgggc tggtggtcca gatgggcaac cattcctgcg ggatgccgtc
     3061 gatgttcagc attcccggcg attatgcgtg gacggccttc tacgatgaac aggggcgcat
     3121 tcttgtgcag aacgggcccc agggtgaaac ttcataccgc tgggacgaag aatccggtga
     3181 actggaatcc atcacgaccc cggcgggacg cggcgtcagc ttcgaatacg atgacgacgc
     3241 ccggatgaca gcgatcaccg tcgatgggat caggatccac tcatacgcct atgaaagcgg
     3301 ccttcgtctt aagagcatcg aggatgcgat cggtcgcacc accgaatacc ggtatgacga
     3361 caaaggcaat cttttcaagg tgattcagtc cagcatcccg gttgcgacct ttgaacgcaa
     3421 cgaaatcggt gaaatcacgg caataaccga cgcaatcggc ggacgtaccg aattcggact
     3481 agacccgatg ggacgggtgg taagcgtcaa ggacagggcc ggcgcgttcc actccatcgg
     3541 actggacgcc atgggacagg tcatttcact taagagcgcg ggccagggac ttgatgatga
     3601 aaagatgacg acatggtctc gtgacgggaa ggggcgcatc caatccgttt cccttctggg
     3661 ggcacctgaa tggactatca attccgatgg cgaggatcgg gtgatgtcga ttgcgagccc
     3721 cgggggttcg gttgcagtca cctatgacgg aatgggacgc gtcgtttcaa gaaccatcgt
     3781 aacgacgaac caggccgttc cggacacgac cgccatcttt gaatacgacg ccgacggcca
     3841 cctggtcaga gcagtcgatg acgacagcga aataacctgg gagtacgaac cgcgttacgg
     3901 caacctgctt tcggtcaccc agttttacca gggtatggcg gaaccgatta cgatcggcta
     3961 cgaccaggac gaggacgggt ggataaacgc cgtcgagtac ccggcccttg acggatttga
     4021 tgggggcagg ttcgattact actatcatga accgtcacaa cgggttgagg aaatcaccgc
     4081 gccggacgga atgtacttcg atctataccg cgacgatgcc ggacgtctcg agacgtttga
     4141 aatcgactgg ggtgatccgt tcctgctgga ggccgcatac gacgaaaccg gttacctggc
     4201 gtcgatcacc gcttaccttg attcataccg ggaagacgtc cacatgaact tcgagtatgt
     4261 gaccgacgat gacggagcca tcatgtcgga aaccggaccc agcggaacaa cggcctttgg
     4321 ctacgaccag ttaagacgcg tcaccggtgc gaccaaccag gcagccggca gggacgaaac
     4381 ctttgattac aacgcccgcg gggatcgtat gggtgccgga ttcgaatacg acgacttcgg
     4441 cagggcgatc gccggcgcgg gattcacctt cgaattcgac gaggccggcc gcgtgacaag
     4501 gcgggttgag acggcgaccg gaaacgcggt cgaattcctg tacgatggcg acgacaacct
     4561 ggtgcgcgcc agcgtctatt ccggcggttc catgacgccc gaacatgtgg tcacttacgg
     4621 ctatgatccg cttggcaaac gcatcttccg ggatgtcgat ggcgtccgga cgttcttcat
     4681 acacgacttc acggacgtgg tgcttgaaat aaacgaaacc ggcggcgcgg acgcgttcta
     4741 tctgcacgaa ccggccatgg acaggccgat tgcgatgtat cgggacggcg aagtatatgc
     4801 ctgcattcag gacggattcg gatcgatttg cggcctggta aggctttcag acagatctct
     4861 tgtctggacg cgggaatacg gggtcttcgg ggacatcctg gatcagaccg gaacaatccc
     4921 gttcaggtac ggcttccatg gacgggagca tgacccgctg acaggcctga ttcacttcag
     4981 ggcacgtgaa ttcgacccgg cagtcggtcg cttcgtttcg acggatcccg aaaaattcac
     5041 atcgttcgcg ggcacttatg cctttccagg gaatgatccg gtgaaccgac acgacccgac
     5101 tggtgccggc cccagggcgg ttcgggccgc gggcgaggtt tacagttatt acaacaagac
     5161 caagcagcac attgagcaaa gcatcgattc aagggtgtca tccgttgccg gcagggatgc
     5221 cggcgccgct tcgtcattcg cctggaagac ggcgggggct ggaatgcttg acagcggggt
     5281 gaagatactt cttccatccc cttaccagtc acccggtccg agcgtcacgg acgccgccgt
     5341 ctgggcatac aaggcctaca aggccaagga tccctgcgcc agggccgcgg tcggcggcga
     5401 cgtgctggtc gatatcatgc ccgcaggcga caaatggctg cggccgatgg tcgataaatt
     5461 caacaatttc gggaatctga tgaattcggg gaactgaccg atcaaggcag atccgcggga
     5521 agaagggact gtccaccgat cccgacatcg acgaccatgg cccctcgctc cgacacaaca
     5581 agcaccacag caagcaggga catgtaaagc ctggacgaac caaactcgtc ataaccccac
     5641 gcgatatttg cgataagccg gtccggagat gtcgcgccgt cgggaaccgg ggccgaaatg
     5701 accttgtacg ggggttcgtc cgccgaagcc gggacgaccc agacgctgct gtccgtcaac
     5761 gtgaatccat cgaacatgtc gaagatcaca tacatcgtgc cgtccgcatc gaaggcaagg
     5821 ccgtcgccga acatcgcgaa gccttccagg cggggggtcg gctggacggg gccacctgca
     5881 aggccgccac tcaaatcaat cgaatagagc ccgccatggt taagggtcac gtccgcgccc
     5941 gaatcgggac acagaaggcc tacattctcg gtcgttaccc acagcaggcc gctgccgtca
     6001 aatgcaagcc cgtttggacc gccacgaacc gtcacatcga acatcgcgac agtttcaagc
     6061 acaacggtgt cggcatcggg ctggaagcga tataccccgc cggtgcatga atccgtgaaa
     6121 tacacatatc gattgtcggc aatcgccacc gaattgggcg tcacaagacc gtcaaaacca
     6181 gccatcgtcg tggcggttgc cgtgattgtg cccccaacct cggaaatgct tacctttcgc
     6241 accgaaccgg catcggcgac ccaaatcacc ttgcgaagat aatcccaggc cattccaagc
     6301 ggactggtca ggccatcccc gtcgaccggc acaagctggg ccgtgccatc cggccgcatc
     6361 gccacaagat tgccgggaac cgccaggaac aggcgaccgg catcgtcaaa aaccagccct
     6421 tcggtcgacc catcgatgcc ggcaccttca agcaaggccc cgacatcccc gaacggctgt
     6481 ggaaccagcg gttgaaccgc ggccacgtca acatgatggt cgatgaagac tgccgcctgg
     6541 tcgattgatg cccgcagaac ggcccggcct gaaatcggtc ccgccgtcca ggtcgttgaa
     6601 gcaattcccg aagcgtcggt gacgacggct gcctgactga tcatcccgcc accgaacacg
     6661 atttcaaagg acaccgtcac cccggactgg gcgacatcgc caagcgtgac ctgtgtcgaa
     6721 agctcaagct ggccggaagc cgccactgtt ccgggcatgc cgtcgccaac ccattccagc
     6781 ttccactgtt ccccgcccgc atcatttccg gaatcggggg atccggctgt cgaatcgcca
     6841 cagccgcaaa gaacgacgaa cagagtcgcc acggacaacc ttgttgcaac atcagcggtc
     6901 ttcatcggaa tccgcctccg gattatcagt cgtgtggaag tctagcagac ggttgcaccc
     6961 cccgccataa cagggaacga agtaatcttt tgcgcatttc agccaaaggg ccgattggcg
     7021 gtatcattcg gctgggaaac gacgtggaac aatccaatgt gttcaaccgg cattttcgag
     7081 atcatcatgc tgctgtgctt ttcggcggca tggcccttca gcatcgcaag gtcgttccgt
     7141 tcacgaagca caagtggcaa gagcctggtg ttcctgctgg ttatccttgc cggatatgcc
     7201 gccggaatcg tccacaaggc caactgcaac cgtgacccgg tgatatccct ttacattttc
     7261 aacggttccc ttgtgctgat cgacatcctg ctgtacctta gaaaccgtat cgtcgaaagg
     7321 cgggcctcgg tggactcatc gcaggttcat ggacggactg aaaacactcg gggggaaatc
     7381 gaatgaaggc aaagatcacg attctggtgc tggctggaat gttccttctt gtggccggct
     7441 gtggcgggga taacatctca accaacgacg tgcttcaaga catcttcatt cctttcgaca
     7501 ctaaccagcc cgacctcgtc gtcaaccctg atgcggacga tcgggatcag aacgttccgg
     7561 tcgatcacgg taccggcacg gacaccaacg tgccgacgga caccaacgtg ccgacggaca
     7621 ccaacgtgcc gaccgacacc aacgtgccga cggacaccaa cgtgccaacg gacaccaacg
     7681 tgccgaccga caccaacgtg ccaacggata ccaacgtgcc aacggatacc ggaaaccaga
     7741 ccgacaccgt cgatgtcgag ggatgcaccg gttcatgcac ttacggaacc gacgccatgt
     7801 ggtgttccga agatgggctt tccgtgtgtt tctgcggcga ggacggggaa tggatggcat
     7861 tcaactgcgc cgacgcatgc gccaacagca acatggtcgg ctcacagtgc atcaatgacg
     7921 acggggccga atactgcagt tgcgaattcg actgcaccag aacggatctg acgacgtccc
     7981 agtgcaacgc ttattcatac acgccgtgca catgcgcggc tgccgacccg tgcaactggg
     8041 acaatgacgg ctggtgcgac aagatatgcg aactggaatt ccccgaggat tccttcaccg
     8101 aaccgaacga ctgcgactgc ggcggcgaat gcgacccgga tgaaggaatc gcgtactgcg
     8161 acaacgatca ggggctgtgc tggtgctatg agggatactt cgaatcgctg ccttgcaccg
     8221 acttctgcga ttatcttggt gccgagctcg acccggaaca gtggtgcttt gtggatatga
     8281 gcgaacagta ccagtgttca tgcgtcaatt acagttgcgt tgacagcgac gcggtccaga
     8341 accagtgtga cttcggcgtg tattcggaat gcacgtgcgc ggctgccgat ccgtgcggat
     8401 gggccaatga ccagtactgc gataccccga cctgccagta cctgtacccg gagttgtcgg
     8461 tatttgacga caccggctcg gcagcctgcg gcacctgaaa cttcccggcg caatcttcat
     8521 caacattttc attcgccagt acggggcatg gtgtggatcg gatcgtgggg cggattgggg
     8581 cggttccagg gggatattcc ggagtacctg gatacctttc tggatatggc gccacggatg
     8641 ctttcgtcgg ttccgtgtat ccagacattg ttctttgccc gggtcatggc cgtgtaaaga
     8701 atctcgcgga caagcagtgg atgatccgcc ctggatggca ggaaaagcag cacatttccg
     8761 tattcggagc cctggctttt gtgcaccgtg gttgcgaacg ccggttccag acccggaatc
     8821 gactgcaccg ggaatgacct gaagacgacg ccgtcatcaa agatggcgac cggctggccg
     8881 tccagatcat tcatgaccat gccgacatcg ccgttaaaga gcccctgggc cgcgatattg
     8941 cgggtaacca gcaccgggca cccggcaaac aacccgtcgc ggcccccggc aaacaacccg
     9001 tcgcggcccc cggcaagcct tgcgattgcg cggttgatgc cgaccacacc ttccggcccg
     9061 tcgcgaagca cgcacagaat acggcccatt tgcagtgccc tgaaaagtcc gttcatcacc
     9121 acagcgtctt ccgaacccgg cgcgaaccgc gtatcgagcc ggcgggctgc cagggcctca
     9181 aacgcacggc caatagagcc ggcttccggc cgacaaccgt aaatccacga aacccaggat
     9241 tccagcatcg ggccaatccc gccctgtctg gggtaatccg gtcgcaggat cacgcatgcg
     9301 gcaccaggaa gcccccattc gtcgcgatcc ggaacgcggt cggcaaggcg accggcccga
     9361 tcaagaaatc gctggccgtc accggcattg gcgacaccgg caagcgaaag tacctggccc
     9421 ccggctcgat acgactgcga caacattacg atcgaatcct tcatcggccc ggacacgtcg
     9481 ccacgcggga tgatatcggc cagaaccgtg ccggaatcga ctgatggaag ctggtcgcga
     9541 tcgccaagaa agacgacgcg cgcgtccgga cggactgcct gcagaagacg gttcatcaac
     9601 gcggcgtcaa ccatcgacac ctcgtcgcag accacatacc gatgcggcag cgggttgtcg
     9661 gaaccaaacc tgaaatcgtt ccaggacggc ctgaaaccaa gcgccctgtg tatcgtgctt
     9721 ccggtcaggc cacggatgtc ctcgacctgg aactcgtcag gaccgattat tgcgtcgtca
     9781 atggcggcgc tgatcgactc acccatgcgc cttccggcac gaccggtcgg cgccaccagc
     9841 aacatctcgc cgggaaccgc cccgcacaga caccaggcgc gcaacaatgc ggccaggatc
     9901 ctggtcttgc cggtaccggg accgcccgac accacgacga acctcttcac aagggccttc
     9961 ataaccgcgg acttctgccg ttcgttaaga tcccgccctt cccttccggc cccgacctgg
    10021 ctgacaacgc tttccagatc cattcccgac agcgggctgg acacgaactg gccgccaggc
    10081 gtcagggcaa gcctttgcag tgcggcattc agcccggttt catggacgtg gtgccgggaa
    10141 aaccacagaa atcgtccatc ggtgaccagc ggatacggat cccacccatc cggattcgca
    10201 aaaagcggca atccatcgtc aatcagggac gccgcgcgcg ccgccacgtg gccggcggac
    10261 ggcagcgaat cgacaacgtc accggttgtc gcaagcaggc ggccgaactc ggtttcaagc
    10321 gtgcccacgt caagcggaag acacagactt ccctggttgc acacggaaaa aagaccggcc
    10381 atcaggcagt tcacggcaaa atcggcctcg gcgtccgcaa cgcgccatcc ggcaatcgca
    10441 agaatatcgc gacaggccag cgcatcatcg ggcgagatca ggtcgttttc gacggcacgc
    10501 cgatacatct cggccacgcg cgcatcaatc atgaacccgg gccgccagcc cagcgcgtcc
    10561 agccacgaaa ggcctttcgt cattcgcgcc cccttccggc ggcgtgcgcc gcgctgcggg
    10621 ccagcagctt ccggacgaac agccttgact cggaaccgtc cgtatccgta aagatttccc
    10681 ggctgttcca gaccccgcga cctggcgggc ccatgccgcg gacgaacagg aacagcccgc
    10741 cggcaagcag gtgtccggaa cccggcccga acaccgattc aagccactga agcatcgcga
    10801 ttcgataaac gtgggcctgg acgatatagc cgttgtcacg catggccctt gatatcgccc
    10861 cggcggacca gtccggattc cgcccgcctt cgtccctgac cgaggtcgtc ttccagtcaa
    10921 gaaaccagaa tcgaccgtcc ttctggaaaa caaggtcgat gacgccgttc agcagcccgc
    10981 ttcgccacgg tggaattcca tcggccatgt tgccgggcgc cagcgggccc gaaccgaacc
    11041 gagactcgtc aagatggaat tccacttccg cgagcgtgtc ggatgacgac agatccgaaa
    11101 tgctgaaatc gagcggggcc gaatccccca gatgtccacg caatgccgac cagaccagcg
    11161 aggcgaactg cagttcggac gaatcgacgg aaccgtcagg tcctggctgc ccgaatttca
    11221 gcggaacgcc gttgcttttc atcttctgga tgatcaggga accgacgttt gacgccatta
    11281 tggcggccgg ggaatcaagc cgggcaaaat cactgaaatc caggggattt ccgtcatcgc
    11341 cggcgcgccc gcaggcgata tcctgcatca actggtgaaa cgcgtctccg gtgtgccgtc
    11401 cggccatcag caggggcgta gcctggatcc cggccgcatc ggcggtggtg ggcgcgccga
    11461 tcatgggttc aatcgcctcg tcgtcggtgg catcatccgc ccttgagccc gccatcccct
    11521 cgacatcgaa ctgggctcgt cccgcaagcc cggtcagcga cgaatacgaa tcaacccaca
    11581 gacggcgcag cggccagcga atctgcgcgc caggggtcag gtcgggcagc attggatggg
    11641 gtgaagcgga tccttcctga cgctttccgg aatcatccac gcagacgatt tcgggggcta
    11701 ccgacccgat cggaatgccg ccacaatcca catcgcttcg aacccgcccg ccggattcgg
    11761 tcaactgcgc cagcctggcc agcccggcct tgatgaaggt gcatatcggg gcgccgtcgt
    11821 tcttcgatgg cgtccagtcc gttggcacgt acagcttgta catggcccgc gtcagagcca
    11881 cgtaaaacag ccttggaagg tcgttgtcct cgacaagtcc gggataaggt cgttcaaaac
    11941 tgaaatacct gtcacggccg atatagcccc tgcacgactt ccccgacctg gggccggaaa
    12001 agcccgccgc gacgaacacg atcgggaact cgagcccctt tgccgcgtga atcgtcaata
    12061 ccctgaccct gggctgttcc gattccagac gggccattgt cgattcttcc tcgtcacctg
    12121 aaccggcctt caactgctcc atgcgaccga tcagcccatg aagatccagc gaaccggaat
    12181 cggcgaacga agcaagttcc ccgaatatct gcctgaaatt cgcgagccgt ctttcaccgt
    12241 cgaattcggc cgcttcccgc acgaaaaggg ctgtatcgcg cgaaattgcc tcgaagaaca
    12301 tcgcccagtt ccgatcaatc gccagggccc gccaggcttc aagagtacgg gccgcgtggt
    12361 tccgccgttc gggttcaagc atgtcggccg gatccgggcg atcaaggaag aatctggtaa
    12421 gaagcacgga atcgattgac tgggaacctg gacccgcacc aagccgtgaa atcgccttca
    12481 gcaggacaag cacgttttcc gcctcggccg attcgtaaag cttcttctgc ttgtactggg
    12541 cgtgcgggat gccctcggcg cgcagcagcc ttgaaatcag ccggaagtca tcgttcttct
    12601 ggataaggat gcagatgtcg tccaggccaa gcggccgcgg cccctgaccg gcccgcccgg
    12661 gcagaatgat cccgccggat ccggtcagcc gggagatctc cttcgcgatg aatcgcgcgg
    12721 ttctgtaacg ggcctcatcg acctccagct tgcccttttc accaggcctc atgatgtgaa
    12781 cgggaaccac cgcggcccgc ccggacgcgt cggccaccaa atcctgcggc cttccatccg
    12841 ccggggccag caattccgga cactcgatgt cggtcccgaa ccagccggtg tcccggaaaa
    12901 ttccgttaaa accttcaatc atcggtacgg aagaacggta gttcgtctga aggctcaaga
    12961 tgttgccgcc gcgccgctca atttccgaac aggctgaaag gtacgttcgc aagtcggcgc
    13021 cacggaagcc gtaaatggcc tgcttcgggt cgccgaccac aatcagccgg ttcggtccgc
    13081 ccccttcaac aaaaaccgtc ctgaatatgt cccactggtc cgggtcggta tcctggaatt
    13141 catcgacaat gccgaacagg aaccgcttcc gcaggcgttc gagcagggcg cgccccgccg
    13201 gccccggcat ggtcagagcg gatctcacgt cggaaatcat gtcgccgaaa gtcaccagcg
    13261 acgatgccat cttttcccgc gccacggcgg cgcaaagggc ggaagtggtc cacgcaagaa
    13321 tctgggcggt gctgaccgca tccgccaggt caccgtcaaa ttccggcagc aggccgtcca
    13381 gggaccgctt ttcccccgcg acattcaaga cttcgcgcaa cctgcccaac gcttccgaag
    13441 caatcggcag agtacctttg gcgttcatcc tccgggggtc aatccgcggc cattcatccc
    13501 tgacaatccg gttcaacagg cgcgaccgca tatcggaatc gccgccctgc tgaagctcaa
    13561 gcggacggga attctcgaaa gcaaactcca tcagtacacg attacagaat gcgtggattg
    13621 tcgatatcga cgccgactcg aaatcgtcca gttcgatgcg cagcctggcg gccgtggcgg
    13681 gcgaaacgtc cttcccgccg gccgtgtcca tcaaccgctt gcgaatcctc tgcttcagtt
    13741 cgccggccgc cttttcagtg taggtgacca ggagtatgtt gcgaagcgaa acatccggaa
    13801 aatccagaag aatctgaagg acgatgtgtt caagggtata agtctttccg gttccagcgg
    13861 atgcctggat gacggcgtgc cgatcaatga cgccggaaag gtccatcgtg acgccggtcg
    13921 aactggtcag cgggctcatg cgccaccccc gccgcaacgc cacagcaggc cgtaccgcga
    13981 ttcaatcgtt tcccggatcc gttccggcag cacggtccga tcccagacca gtttctgcaa
    14041 agcgttgatc ggctgggttg cccagccacc ctggctgccc ttttcaatcc tggcccgcat
    14101 cgcgcgccgg aagtcctcgt cctccggttc gtcggggctt aagcctgcat atttgaccgc
    14161 cacgtcatac ggcaggtttt ccaggatcct gcgacagtca aggtaatcgg aaatcaggtc
    14221 ttcaatccat cccgcggcgg ttgaaaccgg caccttgatc cgcctggcga cgttcttgtc
    14281 ggcacacacc acaacggcgc cgaagtcgat ttcacgccgc ccggcgccga ttccggccac
    14341 gattccaaac agggtcggca tgatcaatgc ccgggggaac ggcccggttt cgctggcccc
    14401 ggaagcactg acatttccga taatcaaggg caatccatcc agatccggaa ccacgaaatc
    14461 gacccgcccg gtcacttcga cggccatgtc gatactgctt ccgtccggtt tcgatatcct
    14521 ggccgagaac gatgcgggcg caagccgcaa tcccggagcc ccccttgaga aggaaacctg
    14581 ccccatcacg gcctcggaaa ggggtttcat gcccccaatc gaatccatga tcgcttcggg
    14641 tgtgttggaa agtgccttcc tgacgcccag ttcggaaagc tgacggaaat acg
//
LOCUS       DAOPPA010000013        14438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4908_length_14438_cov_4.302162, whole genome shotgun sequence.
ACCESSION   DAOPPA010000013 DAOPPA010000000
VERSION     DAOPPA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4908_length_14438_cov_4.302162"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKH54_00915"
     CDS             complement(<1..378)
                     /locus_tag="PKH54_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98474.1"
                     /translation="MTTKNPIVANALERLGASEFSMSAAPLADTLWAVKRLLGLTEWQ
                     SIDEVPSIAVVLGSGLGGFEERVDADFEVPYRDIGLPAPGIQGHSGKLVIGSIGTRKV
                     AVLAGRIHYYEGHSFEAVCRAVRM"
     gene            456..1592
                     /locus_tag="PKH54_00920"
     CDS             456..1592
                     /locus_tag="PKH54_00920"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOC98475.1"
                     /translation="MAKQVLEIMVSFAPPAAGLSLSRDAFRRRSTTTVDDMIYRHILR
                     PILFKFNGETAHHLAMIGLGIASVVPGCPRIMRSLMAPRSAVPVKACGVTFPNRLGVA
                     AGWVKNGDALPAVWGLGFGFVEVGTVTAGAWGGNPKPRVHRLVRDEGMINRMGLPSQG
                     WEPVRHRLEGRRPSMPVIVNVGKTASPDIHGDAAVADIVTTVRGMAPVADMLVLNLSC
                     PNVTDGRNFENDADALTGLLTAVRAALAGAKVPLLVKLSPDMAAPDLQRIVDVSMACG
                     VDGFVASNTTKSRDGLVQSELAAGLAGGLSGRPLRDRAVATVSAVRNRVGPAPLIIGC
                     GGIFDGRDADMFMDAGANLLEGFTGFIFQGPLYCRQVIDTMRAG"
     gene            complement(1619..2965)
                     /locus_tag="PKH54_00925"
     CDS             complement(1619..2965)
                     /locus_tag="PKH54_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC98476.1"
                     /translation="MGRIRTGQMTLAVAALFAIAIAGCGGGPSFIGGHDHNAFYDFGG
                     TPDAGTRDDGSRDTGVTDDGMTQDNVSDGDADPGDDDGSSRDAVRDEHGGQDDASRDQ
                     GERTDSNTPTDQGGVDTGTDDPPAVFVADLGSGSLNNWETRAKALIGGARNSIDLAHL
                     QFLMYSPDDISIADSIKAAAARGVKVRVLLDDDVDSSAARVNDLNSASRNIDAKVDDW
                     SGTTHVKLIVVDDAQVLVGSTNLSKSALYHNNEANLWISDPAAVGEFARYFDSLFNDP
                     GSQASMYADWSNGVLPIGDDEYEDEVSPLIDSAGTRVLLAMYDLNQDSAAASRLTDKL
                     GDAARRGVDVRVVLERCNESWAYYVTEDNLESADILRDAGVDVRFDEPSGETDRVVTH
                     TKLLVIDDAVVVYSGNWNNSGLRNNHEAGAIVTGVRQVSDAATTYFNSLWNEGSAQ"
     gene            complement(2938..4011)
                     /locus_tag="PKH54_00930"
     CDS             complement(2938..4011)
                     /locus_tag="PKH54_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98477.1"
                     /translation="MNREREHEGVGILRQEADDVGQRVHICIGAICNNNCIFCMEDDR
                     QARRDAVEAWSKADVLDVIAANADRNEILFTSGEPTTNRDLPEYIAAAARAGYRVIGV
                     ISNGRLFSYAPYAVSLLKAGLNNIIVSIHGPDAKTHDALTRTKGSFEQTLAGLRNLST
                     LKAAFPLKLQTSTVVTTRNLSSMRAFCDLMADFDIDAHAMNVMMPDGGGRKHMARLMP
                     RYRDVAAYFAGIIDEIDTRVAGRMALVDIPYCATVGLPDSIRGYVERYFHFEADGEFE
                     RKAALAGVHRNDSIDESPELFVRNAMDGSSRVINRVTRTYQESWVKTKRPECALCRHD
                     PQCRGVWKAYVQEFGWDEFEPVR"
     gene            complement(4008..6338)
                     /locus_tag="PKH54_00935"
     CDS             complement(4008..6338)
                     /locus_tag="PKH54_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC98478.1"
                     /translation="MNGFPNRYRAYLFQWLTGAAAAGLVDGVLAAVTSTGSRLPVLSS
                     VYALTGFILVLAAPIMALLSMSLIQDPGDMPGLPRRIGRMLYPEDPGARKCKALSMLS
                     AAILAGVFMVAVTLASISFFESMRTGGFAAVATAGTALVAGVVLIGIQGRIGAACLRL
                     PPPGKAGPAAILVSPAGMIVLFCLVATVPAILFRDRLIDIFKALPIPLIAIGMTYVGV
                     SVATGIAARFERIPRAALIAVFIGIPVTVTSAIGVCLTGTGINHEVKRSIMSDAVIAP
                     HSYRAARLLLDFDRDGFMGLMADGDCAPLDPNIGPGVPERPNNGRDEDCDGEDLTFDE
                     ERDTFWGRWDFEVPPAIRGRKYNIFLITIDAAAPARMSLYGYDRPTTPFLQEFASTAA
                     WFRSAWSAGPSTRLAIPEMMTSKYGPQVDRATGFRVPLEIRQGNTTMAEFLKRAGFRT
                     YAFLPTSYFSNWKGMTQGFDTVDKSAVAFDRRDRQNHTGDKVTEAFIAGMEKASKDKV
                     RPVFMWAHYYDPHSPYTRVRGGADFGAARPDLYDAELAFVDSQIEKVIKEIDSRFEPE
                     RTVIIISADHGESFDANHATKHHGHDLHSSILHIPLLIRAPFVKPGVFDGPVSALDIL
                     PTLLNIEGIKGRHKLAGTSLVPQLVEGKDDPDRTIYSMFYLPENIYHDLNPDIMIGVR
                     TREYNWFKEFETSVEHLYQYLEDPFEERNLIDDMPFKGREMRRKGAKFMLWLERNSPG
                     NARGDKPGQPPPTTTGKAASPPKAPLEHRRDGKLKP"
     gene            complement(6357..7409)
                     /gene="rlmN"
                     /locus_tag="PKH54_00940"
     CDS             complement(6357..7409)
                     /gene="rlmN"
                     /locus_tag="PKH54_00940"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOC98479.1"
                     /translation="MNSNESPRIELIDLLPEEIGGLPAFVDEQPYRARQVFSWIHDAK
                     VRDFDGMTNLSLAMRTRLKETCSVFRRDPVQIQESVDGSKKLIFLTDEGFSYSAVLMP
                     GLERNTLCISSQVGCRMNCAFCMTATLGFKRNLSAAEIVGQIHAAATLLVPPSRVSNV
                     VFMGMGEPLDNYDNVLRAVRLATHREGLKVAPRRTTISTVGLIDRVAAFVNENTGASI
                     AISLCATTDEARNRVVPQGRRFGIDDIVSGLAGIRLAHGHVFTIEYSLIKGVGDSPAD
                     ARRLSAMAARFPSKINLIPFNPWPGCHFERPDDATIEAFREILERKHHTVTVRISRGQ
                     DIGAACGQLDGCKSTG"
     gene            complement(7437..7901)
                     /locus_tag="PKH54_00945"
     CDS             complement(7437..7901)
                     /locus_tag="PKH54_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOC98480.1"
                     /translation="MTATEIVFSAAHLLFFPPSGREPIHGHDYLVTVTGISSSPAPGA
                     RETLHLLAREAIAPMEHRVLLASRPATGCVTVAEGSVRIEGDGRLIVLPQEDCFTLDD
                     TANSTTELIAAHIARKMAESMRARAAGFTAVEVDVLEGRDCRARVTVHVPGI"
     gene            complement(7944..8297)
                     /gene="queD"
                     /locus_tag="PKH54_00950"
                     /pseudo
     CDS             complement(7944..8297)
                     /gene="queD"
                     /locus_tag="PKH54_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
     gene            complement(8294..9721)
                     /locus_tag="PKH54_00955"
     CDS             complement(8294..9721)
                     /locus_tag="PKH54_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98481.1"
                     /translation="MPNRPHKRYSTGSNAPERSGDARNQAATVLRIDGPAHLGQGVGR
                     LDGKVWMIPFSAVGDTVMAVPEKDHGSWIAGRLVSIAQPGPGRVEPFCPVYGMCGGCH
                     LQHIDAQAERQWKTTVLRDVLERIARIEPGPVRYLAGSDRSCRCRVLMHAGGGRGTRD
                     AGFFQAGSHRIVRPGQCPVAVEPVARLLEPIRQLVRNGGGADDATIEPVCGADGKVVV
                     VVNQESGDPTDLCEAIAGLPGVSGVLFRGGRPGWRKIGAGSVRWPTAGLPGQVEYIDV
                     DPRGFTQSHIEMNPTLVETVIGALGDGIQGMKVLELFAGSGNFTLGLAMRGASVVATD
                     INAAAVRAAADGFESRGLHVDHESGDIEDVLSRMVAGFGRPQAILADPPRSGLGRQAA
                     AVAGLKADRVVLCSCEPSALARDLGPFLLAGYGIDEITLVDMFPQTYHMETVISLTFN
                     RLCGESPFRSGNAKPDSDRNPGDLT"
     gene            complement(9721..11451)
                     /locus_tag="PKH54_00960"
     CDS             complement(9721..11451)
                     /locus_tag="PKH54_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOC98482.1"
                     /translation="MKKTFAIILVACAAPLMMTCAGPQKKTGGGPLDPASEGSRMDML
                     RQMRSQFANVEISVNREQIRPGDLKVLPHLVKAAAIMDRIFWHQASSDGLAIRDALAS
                     RGDELSRLTVQLMDINFGPWNRLDHFKAFHGDRPRPEGVTFYPADMTAAEFESFIAKH
                     PDEAAAFQSWLTVIVREDGSLKAVPYSVAYAADLAEAATHLRAAAKATGDEVLARYLN
                     GRADAFETNDYLQSDMDWMDLGQSSGEGASGIEVVIGPYEVYEDSLLGLKAAFEAFVT
                     VVDEAESRKLSMVAGMLDELESNLPVADHHKNFSRGKMSPIKVVNVVYTAGDTKAGVQ
                     TAAFNLPNDERVRSLKGSKKVMLRNVTRAKFDKALIPISRAVLDPALLDLIDFDAYFN
                     FILMHEVSHGLGPGVISMPDGSKTTVNLALKDTYSIIEECKADILSVHNTLYMASKGI
                     FDRPFMDKTEASYLAGIFRSIRFGIGEAHGGANVMQLNYLMDKGAITFDQATGRFGLD
                     RDRFPGAVRELAAEVLEIEAVGDLLSARAMMDKYVNVRPEVKRALDRLGDVPVDIKPT
                     YSLGAEFLAK"
     gene            11657..13567
                     /locus_tag="PKH54_00965"
     CDS             11657..13567
                     /locus_tag="PKH54_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC98483.1"
                     /translation="MRCGKKGPGRFGQALVSFVPRFGCPEAVMAAAVLLLNVIFLSIV
                     TVKNEFDLSDPFVLSSFSVKVVFDLALMFLIVNALRGLLKSAWPSAVFMAFYLLLSLA
                     NIFLYHFGNTMLERHHFALITPYSVTAYIPGWGFLVIFVGVAASILLFGLLFRRFRSG
                     RVLRESAFLLLVLFLLVAYNRQGFFSRTDDEKLDRVIMGFRNAQVHYACRNQFLSLVK
                     DVTFPALGSRLKALAPRTESFVDDYDLTSSRFKVISELDPHRRAIDAFNVPIGRGALK
                     SAGLKPFSRIILVAAESMSLDALQCFNPQIKAEYATRFFCRPDIVAATFSNIFTTGSP
                     TLQGLTVIFNGHPNFMIQEQTGHPGSFPKLLADNGFRSVFIRSASKYFANENLVFRNM
                     GFGTIIGREDFFEDESLRKYIYGWGLEDRILYRKALEYIEQHRDEKLFVTVFGTDTHP
                     PYGQMHFKHLKYPPRPELRGSVPPDTYEWLRAVDAMDHDIDQFIRDLDAKGLFDERTL
                     IVVMADHSCPVNNVTAKIPGHPRTNLARIPVVFLSRQPLPEADFATLGSQVDIAPTIF
                     HLAGLNGSEGWWGDSLFNPDRRSWSIGFDKGFVSISDNGKEVLMNVDKPSDDSQRSFL
                     DLFYSVMAPDRD"
     gene            complement(13576..>14438)
                     /locus_tag="PKH54_00970"
     CDS             complement(13576..>14438)
                     /locus_tag="PKH54_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98484.1"
                     /translation="RSTRPIGDITLAVRRLAAGDLSHVKIRASRELEETADAINHLAS
                     TFRAQREAGAVEREREVRTERLAVVGRMASAVAHEIRNPLNSISLSIDLLADFMESPE
                     HREGRGREVIATVQREIDRLNEITEDYLQFGRMPKAVIGPCNVTAVVKETCDFMAREM
                     REGNVAVTIDAFDNTARVLSDEGQLRQALVNILRNSVEAMPEGGTISIRLSAVADGIE
                     LTIADTGQGIPDSQMARLFEPFATTKRNGTGLGLGFVQQVVTESGGKVRIESGQGKGT
                     TVRITLARAA"
BASE COUNT         2692 a         4467 c         4232 g         3047 t
ORIGIN      
        1 catccgtacc gcgcggcaga cggcctcgaa ggaatggcct tcatagtaat ggatgcgtcc
       61 tgccaggacg gcgaccttcc gtgttccgat tgaaccgatg accagcttcc cgctgtgacc
      121 ctggatcccc ggggccggaa ggccgatgtc gcggtaagga acctcgaaat cggcgtcaac
      181 gcgttcctcg aatccgccaa gtccggatcc gagcaccacg gcgatagacg gaacttcgtc
      241 aattgactgc cattcggtca gtcccagaag tcgcttgacc gcccagagcg tgtcggccag
      301 cggtgctgcc gacatggaga attcagatgc cccgagacgc tccagggcat ttgcaacaat
      361 tggattcttc gttgtcatct gtgtccttcc cgagttgcag gggttacttt acaaaccctg
      421 gccattctaa cccggatcgg aaccgcgggc acaaaatggc gaagcaggtg cttgaaatca
      481 tggtatcctt cgcaccgccc gccgcagggc tttccttgtc acgggacgct ttccggcggc
      541 gttcgacaac gacggttgac gacatgattt acagacacat cctgcgcccg atattgttca
      601 agttcaacgg tgaaaccgca catcacctgg cgatgatcgg tcttggcatt gcatccgtcg
      661 ttcccggctg cccgcgcatc atgagatccc tgatggcccc gcgcagcgcg gttccggtca
      721 aggcctgcgg cgtcacgttc ccgaaccggc tgggcgtggc ggccggctgg gtgaagaacg
      781 gcgacgcact tccggcggtc tgggggctcg gcttcggctt tgtcgaggtc gggacggtga
      841 ccgccggtgc ctgggggggc aatcccaagc cacgcgtgca ccgcctggtg cgcgacgaag
      901 gaatgattaa caggatgggg ctgccctcac agggctggga acctgtccga cacaggctgg
      961 aaggaaggcg tccgtccatg ccggtaatag tcaacgtcgg caagacggca tctcccgata
     1021 ttcacggtga cgcggcggtc gccgacatcg tgacgacggt tcgcggcatg gctccggtcg
     1081 ccgacatgct cgtgctgaac ctttcatgtc cgaacgtaac cgacgggcgg aatttcgaaa
     1141 acgacgccga cgcgctgacc gggctgctga cggcggtcag agcagccctt gccggggcga
     1201 aggttccgct tctggtcaag ctttcccccg acatggcggc ccccgatctg caacgtatcg
     1261 ttgacgtgtc gatggcctgc ggtgttgacg ggttcgttgc ctcgaacact acgaagtcgc
     1321 gtgacgggct ggttcagtcg gaacttgcgg ccgggctcgc cggcggactc tctggaaggc
     1381 cccttcggga cagggccgtg gccacggtct cggcggttcg gaaccgggtt ggcccggccc
     1441 ccctcataat cgggtgcggc ggcattttcg acgggcgtga cgcggacatg ttcatggacg
     1501 ccggcgccaa cctgctggaa ggcttcaccg gattcatctt ccaggggccg ctttactgcc
     1561 gacaggtgat tgacaccatg cgggccggct gacggagatg tccttcacct gatcaatgct
     1621 attgcgccga gccttcgttc cagagtgaat tgaagtaagt ggtcgccgcg tcggaaacct
     1681 gtctgacgcc ggtcactatg gcgccggcct cgtgattgtt cctcagcccg ctgttgttcc
     1741 agtttccgga atacacgacg accgcgtcgt cgatcaccag cagcttcgtg tgcgtgacga
     1801 cgcgatcggt ttcgcccgaa ggctcgtcga acctgacatc gactccggca tcccggagaa
     1861 tgtcggccga ttccagattg tcttcagtca cgtaataggc ccagctttcg ttgcaccttt
     1921 ccagcacgac gcggacgtca accccccgcc gggcggcgtc gccgagcttg tcggtcaagc
     1981 gggaggccgc cgccgaatcc tggttgagat cgtacatggc aagaaggact cgggtgccgg
     2041 cggaatcgat cagcggggat acttcgtcct cgtactcgtc gtccccgatt ggcagaacgc
     2101 cgttcgacca gtccgcgtac atcgaagcct gactgccggg atcgttaaaa aggctgtcga
     2161 agtatcgggc aaattccccg accgcagccg gatccgaaat ccacaggttt gcttcattgt
     2221 tgtggtaaag cgctgacttc gacaggttgg tggaaccgac gagcacctga gcgtcgtcaa
     2281 cgacaatcag cttcacgtgt gtcgttccgg accagtcgtc aaccttcgcg tcgatattcc
     2341 ggctggcgga gttcagatcg ttcaccctgg cggccgaaga atccacgtcg tcgtcaagaa
     2401 gcaccctgac tttgaccccg cgggccgcgg ctgccttaat cgaatccgcg atgctgatgt
     2461 cgtcgggcga gtacatcagg aactggaggt gggccaggtc gatgctgttc ctggccccgc
     2521 cgatcaaagc cttcgccctg gtttcccagt tgttgaggct gccagacccc agatcggcca
     2581 cgaacacggc cggaggatcg tcggttccag tgtcaacgcc accctggtcg gtcggcgtgt
     2641 tcgaatcagt cctttcgccc tggtcgcggg atgcgtcatc ctggcccccg tgttcgtccc
     2701 tgacggcgtc ccgggaggag ccgtcgtcat cgccaggatc cgcatccccg tcgctcacgt
     2761 tgtcctgggt cattccgtcg tcagtcactc cggtatcccg tgacccgtcg tcccgggttc
     2821 cggcatccgg agtgccgccg aagtcgtaaa aagcgttatg atcgtggccg ccaatgaaag
     2881 acggcccgcc accgcagccg gcaattgcaa tcgcgaaaag cgccgcgacc gccagagtca
     2941 tctgaccggt tcgaattcgt cccatccgaa ctcctgtacg tatgccttcc atacacccct
     3001 gcattgcgga tcatgccgac acagggcgca ttcgggtcgc ttggtcttga cccaggattc
     3061 ctggtaggtt ctcgtgaccc ggttgattac gcggctcgac ccgtccattg cgtttcgaac
     3121 gaacaattcc ggactttcgt cgatggaatc atttcggtgg accccggcca gggcagcctt
     3181 cctttcgaat tcaccgtccg cctcgaagtg gaagtaccgt tcgacgtatc ctcgaatgga
     3241 atccggaaga ccgacggtag cgcagtacgg aatatcgacc agcgccatcc tgccggccac
     3301 cctggtgtcg atttcatcaa tgattccagc aaagtacgcg gcgacgtcac ggtacctcgg
     3361 catcagcctg gccatgtgct tccgaccccc gccgtccggc atcatgacgt tcatggcgtg
     3421 agcgtcgatg tcgaagtcgg ccatcaggtc acagaaggcc cgcatgctgg aaagattcct
     3481 cgtggtgacg acggtgcttg tctggagctt caggggaaaa gcggccttca gggtcgaaag
     3541 gttgcgaagt cccgccagag tctgttcgaa gctccccttt gtcctggtga gagcgtcgtg
     3601 ggtcttcgcg tccgggccgt ggattgacac gatgatgttg ttcaggccgg ccttcagaag
     3661 gcttaccgcg taaggcgcgt acgagaagag cctgccgttg gagatgacgc cgattacccg
     3721 ataaccggcc ctggccgcgg ctgcaatata ttcaggaagg tctcggttgg ttgtcggctc
     3781 accgctggtg aaaaggattt cgttcctgtc ggcgtttgcg gcgatgacgt cgagaacgtc
     3841 cgcctttgac caggcctcga cggcatccct tcgtgcctgg cggtcgtctt ccatgcagaa
     3901 tatgcagttg ttattgcaga tcgcgccgat gcagatgtgc acccgctggc ccacgtcgtc
     3961 ggcttcctgc cgtaatatcc cgacgccttc gtgttcccgt tcgcgattca tggcttcaac
     4021 ttcccgtccc gccggtgttc cagcggtgcc ttcggcgggg aagccgcctt cccggtcgtc
     4081 gttggcggag gctggccagg tttatcgccc cgcgcgttgc cggggctgtt cctttcgagc
     4141 cacagcatga acttcgcgcc ctttcgtctc atttcacggc ccttgaaagg catgtcgtcg
     4201 atcaggttgc gctcctcgaa agggtcttcc agatactggt acagatgttc caccgacgtc
     4261 tcgaattcct tgaaccagtt gtattccctg gttcttaccc cgatcatgat atccggattc
     4321 aggtcgtggt aaatgttctc gggcaggtag aacatcgaat agatcgttcg atcgggatcg
     4381 tcttttcctt ccacaagttg aggcaccagg ctggttccgg ccagcttgtg gcgacctttg
     4441 atgccttcga tattcagcag ggtcggcagg atgtccagcg cggaaaccgg accgtcaaaa
     4501 acaccgggct tgacgaacgg agcccttatc agcagcggga tgtggaggat ggaggaatgc
     4561 aggtcgtggc cgtgatgctt tgtcgcgtga tttgcgtcga acgattcccc gtgatcggcg
     4621 gaaatgatga tgacagtcct ttcaggttcg aaccggctgt cgatttcctt tatcaccttt
     4681 tcaatctggg aatcgacgaa tgcaagttcg gcatcgtaca ggtcaggccg ggccgcgccg
     4741 aaatcggcac cgcccctgac cctggtgtac ggcgaatgcg ggtcgtagta gtgcgcccac
     4801 atgaagacgg gtcgaacctt gtccttcgat gccttctcca tcccggcaat gaatgcctcg
     4861 gtcaccttgt ctccggtgtg gttctggcgg tcgcgacgat caaaggcgac cgccgacttg
     4921 tccaccgtgt cgaaaccctg tgtcatcccc ttccagttcg aaaagtatga agtgggaaga
     4981 aaggcatacg tcctgaaccc cgcccgcttc aggaactcgg ccatcgtcgt gttgccctgg
     5041 cggatttcaa gagggaccct gaagccggtg gcccgatcta cctgaggccc gtatttcgac
     5101 gtcatcattt ccgggatggc cagcctggtg cttgggccgg cggaccaggc gcttcggaac
     5161 catgcggcgg tcgaggcaaa ctcctgaagg aagggcgttg tcgggcggtc gtaaccgtaa
     5221 agcgacatcc tggccggcgc ggccgcgtct attgtgataa ggaaaatgtt gtactttcgt
     5281 cccctgattg ccggcggcac ttcaaaatcc cacctgcccc agaaggtgtc ccgctcctcg
     5341 tcgaaggtca ggtcttcgcc gtcgcagtct tcgtcccttc cgttgttcgg ccgttccgga
     5401 acgccgggtc cgatgttggg atccagcggg gcacagtcac cgtcggccat cagccccatg
     5461 aagccgtcgc gatcgaagtc gagcagcagc cgggcggccc tgtaggaatg tggcgcgatg
     5521 acggcgtcgg acatgattga acgcttcact tcgtggttga ttccggtgcc ggtaaggcat
     5581 acgccgatgg ccgaggtgac ggtgaccgga atcccgatga aaaccgcaat cagcgcggca
     5641 cgcggaatgc gttcgaacct ggccgcgatc cccgtcgcga cggaaacgcc gacgtaagtc
     5701 atcccgatcg cgatcagtgg aatcggcaga gccttgaaaa tgtcgatcag cctgtcccgg
     5761 aaaagaatcg ccggaaccgt ggccacgaga cagaaaagga cgatcatgcc cgccggcgag
     5821 accagaattg cagccgggcc ggcctttccg gggggtggaa gcctgaggca ggccgccccg
     5881 atacggccct ggatgccgat cagcaccacg ccggccacca gtgcggttcc ggccgtggcg
     5941 accgccgcga agccccccgt ccgcatggat tcaaagaacg agatggacgc aagagtcacg
     6001 gcaaccatga acacgccggc aagaatcgcg gccgaaagca tcgacagcgc cttgcatttc
     6061 ctggctccgg gatcctcggg ataaagcatc ctcccgatgc ggcggggcag cccgggcata
     6121 tcccctggat cctgaatcag cgacatcgag agaagcgcca tgattggagc ggccagaaca
     6181 aggatgaagc ccgtcagcgc atagacgctt gaaagcacgg gcaatctgga gccggtcgat
     6241 gtaacggcgg ccagcacgcc atccacgagc ccggccgccg ccgcgcccgt cagccattgg
     6301 aaaagatagg ccctgtatct gttggggaat ccgttcatcg ctcgcgatta taggcgttaa
     6361 cccgtggatt tgcatccgtc aagctgtccg caggccgccc cgatgtcctg tccgcgactg
     6421 atcctgaccg taacggtgtg atgcttcctt tccaggattt ccctgaaggc ctcgatcgtt
     6481 gcgtcatccg ggcgctcgaa gtggcagccg ggccagggat tgaacgggat caagttgatt
     6541 ttgctcggga atctggcggc catcgccgac agccttctgg cgtccgccgg gctgtccccc
     6601 acacccttta tcaacgagta ttcgattgtg aaaacatggc catgcgccag gcggatacct
     6661 gccagtccgg atacgatgtc atcgatgccg aatcgtcttc cctgtggcac gacccggttc
     6721 cgggcctcgt ccgttgtcgc gcaaagcgaa atggcgatgc tggcgcccgt gttttcgttc
     6781 acgaacgcgg caacccggtc gatcagcccg acggtcgaaa tggtcgttcg cctgggggcg
     6841 accttcagtc cttcccggtg cgtggccagc ctgaccgcgc gcagaacgtt gtcatagttg
     6901 tccagtggct cgcccattcc catgaagacg acgtttgaaa cccgtgaagg aggcacaagc
     6961 agtgtcgccg cggcatgaat ctgtcccacg atttccgccg cggacaggtt gcgcttgaat
     7021 ccgagcgtgg ccgtcatgca gaaggcgcag ttcatccggc atcccacctg gctggaaatg
     7081 cacagggtgt tccgctccag gccgggcatc agcaccgccg aatagctgaa gccctcatcc
     7141 gtcaggaata tcagcttctt cgatccgtca acagattcct gtatctggac cggatcgcgt
     7201 cggaacacgg aacacgtttc cttcagtctc gttcgcatgg ccagcgaaag gttggtcatg
     7261 ccatcgaaat ccctgacctt tgcgtcgtgt atccacgaaa acacctgccg ggcgcgatag
     7321 ggctgttcgt ccacgaaagc aggcaacccg ccgatctctt ccggaagaag gtcaataagt
     7381 tcgatcctgg gcgactcgtt cgaattcacg gtcaacctcc cgatcaggtc gtcgtctcag
     7441 atccctggaa cgtggaccgt gacccttgcg cggcagtcgc gtccctcgag gacatcgact
     7501 tccaccgcgg tgaatccggc cgcgcgcgcg cgcatcgact ccgccatctt gcgggcaatg
     7561 tgagcggcaa tcagttcggt ggttgaattt gccgtgtcgt caagcgtgaa acagtcttcc
     7621 tgcggaagga cgattaatct gccgtccccc tcgattctca cggagccttc cgccacggtc
     7681 acgcacccgg ttgcaggcct cgaggccagc aggactctgt gttccatggg cgcgattgcc
     7741 tcgcgggcca gcaggtgcag cgtttcccgg gcgccgggcg ctggggatga ggaaatgccc
     7801 gtgacggtga ccagatagtc gtgtccgtgg atcggctccc ttccggaggg cggaaagaaa
     7861 agcaggtgcg cggcggaaaa aacgatctcg gttgccgtga tcctgaagtc gccggaatgc
     7921 tcggcggccg tccggccggt tgggcggtag gtgcaccttg agtaagggct ttcatgaacc
     7981 gtgattgccg acacacctgg aatgctgcgg ctgatcaggt cgtaaaggta cctggaaatg
     8041 ttttcggaag tcgggttcgc aagtccctcg atgtcgttaa gcaccttgtg atcaaggctc
     8101 gaaatgatcc ggccggcttc ccgcgcaaga tcgccgaagt ccatcaccca tccgagttcg
     8161 ggatcgacct cgccgaagat tgccacctca atcttgaaca ggtgtccgtg aagcctggag
     8221 catttgtgat cctgcccgac ttccggaagc cgatgcgccg cttcaaaact gaattcctta
     8281 acgatttctg ctttcaagtc aaatccccgg ggtttcggtc ggaatccggt ttcgcgttcc
     8341 cggaacggaa aggggattct ccacataaac ggttaaaagt caaagagata acggtttcca
     8401 tgtgatacgt ctggggaaac atgtccacaa gcgtgatctc gtcgatgccg tatcccgcca
     8461 gaaggaatgg tcccagatcc cttgccagcg ccgaaggttc gcatgaacac agaacaaccc
     8521 tgtccgcctt gagtccggcg acagccgccg cctgccttcc aagccccgat ctcggcggat
     8581 ccgcaagaat cgcctggggt cggccgaacc cagccaccat acgggacagg acgtcttcga
     8641 tatcgccgga ctcgtgatcg acatgaagcc cccgcgattc gaatccgtcc gccgcggccc
     8701 gaacggcagc cgcgttgatg tccgtcgcca cgacactggc gccacgcatg gcgagtccga
     8761 gcgtgaaatt tccggaaccg gcgaaaagtt ccagaacctt cattccctgg atgccgtccc
     8821 cgagggcccc gataacggtt tcgaccagtg tcgggttcat ctcgatgtgg gactgcgtga
     8881 atccccgggg gtcaacatcg atgtactcga cctgtcctgg aaggccggcc gtcggccatc
     8941 gcaccgaacc ggccccgatc ttccgccagc cgggccggcc accccggaaa agtacccccg
     9001 agactcctgg caggccggcg attgcctcgc aaaggtcggt cggatccccc gattcctggt
     9061 tgaccacgac cacgaccttg ccgtcggcgc cgcagaccgg ctcgatggtc gcgtcgtcgg
     9121 cacccccgcc attcctgacg agttgcctta tcggttcaag aagccgcgca accggttcga
     9181 ccgccaccgg gcattgccct ggccggacaa tccggtgcga accggcctgg aagaagccgg
     9241 catcccttgt gcccctgccg ccccccgcat gcatcaggac acggcatcgg caggagcgat
     9301 cactcccggc caggtaccgt acgggccccg gctcgattcg cgcaatcctt tcaagtacgt
     9361 cccggagcac ggtggtcttc cactgccgtt cggcctgtgc gtcgatatgc tgcagatgac
     9421 agccgccgca catgccgtag acgggacaga acggctccac tcgaccaggt cccggttgcg
     9481 cgattgacac caggcgtccg gcaatccagc ttccatgatc cttttccgga acggccatga
     9541 cggtatcgcc aaccgcggaa aagggaatca tccagacctt gccgtcaaga cgtcctacgc
     9601 cctgtcccag gtgggccggg ccgtcgatcc gaagtacggt tgccgcctga ttccgagcgt
     9661 ctccggagcg ttcgggtgcg ttgcttccgg tcgaatatcg tttatggggc ctgttgggca
     9721 tcactttgcc aggaactccg cgccaaggct gtaggtcggc ttgatatcca cggggacatc
     9781 gcccagcctg tcgagcgccc ttttcacttc gggcctgacg ttgacgtatt tgtccatcat
     9841 ggcgcgtgcc gatagcagat cgccgacggc ttcgatttca aggacttccg cggcaagctc
     9901 ccttaccgct ccggggaatc gatccctgtc gagcccgaac cggccggtcg cctggtcgaa
     9961 ggtgattgcg cccttgtcca tcaggtagtt caactgcatg acgttggccc cgccgtgcgc
    10021 ttcacctatc ccgaaccgga tagacctgaa aattccggcc aggtaggacg cctcggtttt
    10081 atccatgaag ggtcggtcaa agatcccttt ggatgccatg tagagcgtgt tatggacgct
    10141 caggatgtcg gccttgcact cctcgattat cgagtaggtg tccttcaacg ccaggttgac
    10201 cgtcgtcttc gatccgtccg gcatgctgat gactcccggc ccgagtccgt gggatacctc
    10261 gtgcatcagg atgaaattga agtatgcgtc gaagtcaatc aggtcgagca gcgccggatc
    10321 cagaacagcc ctggaaatcg gaatcagggc cttgtcgaac tttgccctgg taacgttcct
    10381 gagcatcacc ttcttggaac ccttcaggct tcgaaccctt tcgtcattgg gaagattgaa
    10441 cgccgcggtc tgaaccccgg ccttggtgtc gcctgcggta tagacaacgt ttaccacctt
    10501 gatcggtgac atctttccgc gcgagaaatt cttgtggtgg tccgccaccg gcaggtttga
    10561 ctcgagttcg tcaagcatcc cggcaaccat ggaaagcttc ctgctttcag cctcgtcaac
    10621 gaccgtgaca aatgcctcga acgcggcctt cagccccaga agcgagtcct cgtaaacctc
    10681 gtacggcccg atgacgacct cgattccaga cgcgccttcg ccgcttgact ggccaagatc
    10741 catccagtcc atgtcgcttt gaagatagtc gttcgtttcg aatgcgtcgg ccctgccgtt
    10801 caggtaacgc gcaagcacct cgtcaccggt cgccttcgcg gcggcacgca ggtgggtcgc
    10861 ggcttctgcc agatccgcgg cgtaggcgac gctgtacgga acggccttca gcgagccgtc
    10921 ctcccgcacg atcacggtca gccacgactg gaacgccgcg gcttcgtccg gatgtttggc
    10981 gatgaaggat tcgaattcgg cggcggtcat gtccgcgggg tagaatgtga cgccttccgg
    11041 ccttggcctg tcaccgtgaa acgctttgaa atgatccagg cgattccacg gcccgaagtt
    11101 gatgtccatg agctggactg tcagtcgcga cagttcgtcg ccccttgaag cgagggcgtc
    11161 cctgatcgcc agtccgtcgc tggaagcctg gtgccagaaa atgcgatcca tgatcgccgc
    11221 ggccttgacc aggtggggga gaaccttcaa atcgcccgga cggatctgtt ccctgtttac
    11281 ggatatctcg acattggcga actgggaccg catctggcgc agcatatcca tcctgcttcc
    11341 ctccgaagcg ggatcgagcg gaccgccgcc ggtctttttc tgtggcccgg cgcaggtcat
    11401 catcagcgga gccgcacatg ccacgagaat gattgcaaaa gtctttttca tcaagtatct
    11461 ccagggccac ggagtgtcgt cggaacaatc ctgcgaaggc gcgccgggcc cataaaacgg
    11521 cgcagggcat atattttgcc accccgtggc caaaaaaaac acacaaaccg gacaacttgc
    11581 ccctaaaatc cccctgtccg ccggcaggtg gaaatcatta agacccggga accgggtgat
    11641 gcggggtact gaaggaatgc gttgcggcaa aaaggggcct ggacgattcg gacaggcgct
    11701 ggtttcattt gttccacgtt tcggctgtcc cgaggccgtg atggcagccg ccgtccttct
    11761 gctgaacgtc atcttcctgt cgatcgtgac cgtcaagaac gagttcgatc tgtccgaccc
    11821 gttcgttctg tcctcgtttt cggtcaaggt tgtcttcgac cttgcgctga tgttcctgat
    11881 tgtcaacgcg cttcgtggcc tgttgaagtc ggcatggcca tcggccgtgt tcatggcctt
    11941 ctacctgctt ctttccctgg ccaatatctt cctttatcac ttcgggaaca cgatgctcga
    12001 aaggcatcac ttcgcgttga ttacgccgta cagcgtgacg gcatacatac ccggctgggg
    12061 cttcctggta atctttgtcg gcgtcgcggc ttcaatcctg ctcttcgggc tcctgtttcg
    12121 gcgcttcagg tcgggcaggg tccttcgcga atcggcattt ctgcttctgg tgctcttcct
    12181 tcttgtcgct tacaacaggc agggtttctt ttcccggacg gatgacgaaa aactggatcg
    12241 ggtgatcatg ggctttcgca acgcccaggt tcattatgca tgtcggaatc agttcctgag
    12301 cctggttaag gacgtgactt tcccggcgct cggttcgcgc ctgaaggcac ttgcgccccg
    12361 caccgaaagc tttgttgacg actatgacct gacttcgagc cggttcaagg tgatttcgga
    12421 gcttgatccg catcggcggg cgattgacgc cttcaacgtt ccgatcggcc gcggggcctt
    12481 aaaatcagcg gggctgaagc cattttcacg catcatactg gtcgcggccg agtcgatgtc
    12541 gctcgatgcc cttcagtgtt tcaacccgca aatcaaggcc gaatacgcga cccggttctt
    12601 ctgccggccc gacatcgtcg ccgccacgtt ttccaacata ttcacgacgg gatccccgac
    12661 gctccagggg cttaccgtca tcttcaacgg ccacccgaat ttcatgatcc aggagcagac
    12721 ggggcatccg ggatccttcc ccaagctgct tgcggacaac ggattccggt ccgtcttcat
    12781 ccgcagcgct tccaagtatt tcgccaatga aaaccttgtc ttcaggaaca tgggcttcgg
    12841 gacaatcatc gggcgcgagg atttcttcga ggacgagtcg ctgcgcaagt acatttacgg
    12901 ctggggactt gaagacagga tcctgtaccg caaggcgctg gagtacatcg aacagcatcg
    12961 agacgaaaag ctgttcgtga cggtcttcgg caccgacacg catcccccgt acggccagat
    13021 gcacttcaag cacctgaagt atcccccgag gcccgaactt cgcggatcgg tgcccccgga
    13081 cacttatgaa tggctgcgtg cggttgacgc gatggatcac gacatcgacc agttcatcag
    13141 ggatctggac gcgaagggcc tcttcgacga acggacgctg atagtcgtca tggcggatca
    13201 ttcgtgtccg gtcaacaacg tcacggcaaa gattcccggt catcccagaa ccaacctggc
    13261 gcgcataccg gtcgtctttc tgtcccgcca gcctcttccg gaggccgact tcgcgactct
    13321 tggaagccag gtcgatatcg ccccgaccat atttcacctg gccggcctta acggatccga
    13381 aggatggtgg ggtgacagcc tttttaatcc cgaccgccgt tcatggtcga tcggttttga
    13441 caagggattc gtcagcattt cggacaacgg caaggaagtc ctgatgaatg ttgacaagcc
    13501 gtccgacgat tcgcagaggt cattcctgga tctgttctat tcggtgatgg cgccggatcg
    13561 cgattgacct gcgcgtcagg cggctctcgc cagcgtgatg cgaacagtgg ttccctttcc
    13621 ctggcccgac tcgattcgca cctttccgcc cgattccgtc acgacctgct gcacgaagcc
    13681 cagtcccagc ccggtgccgt tgcgcttcgt cgtggcaaac ggctcgaata gcctggccat
    13741 ctgtgaatcc ggaattccct ggcctgtgtc cgcaatggtc agttcgatcc catcggcaac
    13801 cgccgacagc cggatcgata tggttccgcc ctccggcatt gcctcgacgg agttgcgcag
    13861 gatgttgacg agcgcctgtc gaagctgacc ttcatccgac aggactctgg ccgtgttgtc
    13921 gaaagcatcg attgtcacgg caacatttcc ttcccgcatt tcacgggcca tgaaatcgca
    13981 ggtttccttc acgacggcgg tgacgttgca tggtccgatt accgcctttg gcatccggcc
    14041 gaactgcagg tagtcttcgg tgatttcgtt gagcctgtcg atttcgcgct gaaccgtggc
    14101 gattacttcc cgcccgcgtc cttccctgtg ttccggcgac tccatgaagt cggccagaag
    14161 atcgatgctc agcgaaatcg agttgagcgg attcctgatt tcgtgggcga cggccgaggc
    14221 catgcggccg acgacggcca gcctttccgt cctgacctcg cgttcccgct cgaccgcccc
    14281 cgcctcgcgc tgggccctga acgttgacgc caggtggttg atggcgtcgg cagtttcttc
    14341 cagttcccgt gaagcccgta tcttaacgtg ggaaagatcc cccgcagcga gcctgcgcac
    14401 cgccagcgtg atatcgccga tgggccttgt ggatctta
//
LOCUS       DAOPPA010000014        14348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_4948_length_14348_cov_3.856363, whole genome shotgun sequence.
ACCESSION   DAOPPA010000014 DAOPPA010000000
VERSION     DAOPPA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_14348_cov_3.856363"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKH54_00975"
     CDS             <1..486
                     /locus_tag="PKH54_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98485.1"
                     /translation="LPAEMKNGFATDATLALQSAFINRATKKFQETLATAFAEKGWFD
                     SETSNIIFRNQFVIPSKAEDPEFNPDSPGFSAALRKGACLPLHAMVSEAVSLSAPMLG
                     LKGIDNAGLNLVFQDLYKKAVAGFAQYGGEKRAPNGQPYLCMIQVPMKDFVASLFRFA
                     E"
     gene            512..2347
                     /locus_tag="PKH54_00980"
     CDS             512..2347
                     /locus_tag="PKH54_00980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC98486.1"
                     /translation="MTNRLRTFDFVAGLFLAGCYFAMLVGTTDIGFSRDEGFYFKAAE
                     EYSWWYDELLDDPSSAFTKKSIDRHFAYNPEHPALPKMLYGLSWRLFGKMHDPKTEPW
                     ARVWYHNSSPPKPILGIMKESTAMRLPALLINSLLVMFIWLFAAEYFSRRTALVASLS
                     WMFLPHAFWHSHLACFDMPVVAMWFLVAWLFLRATPPLEERLRNRRQTWFRAILAGVA
                     WGLAMSTKHNAWFIPAVFLAWYMIARYDEVLPRNGIGRRRLAFGNIPPAFWAMLVLGP
                     LVLFLTWPKLWFDPIEHLKFYIARHAKHEFYWAYFFGFLHVKPPFPISFPFVMSAMTI
                     PVPVILLSLTGLARAFREWIRRRADRFQLIRRPLPVRGNDTILFVFLNFLFPFLIIAA
                     PNTPIFGGTKHWMPGVAFLAIFAGLGFETVLDAIRGLGERFAAFNRRTVRFAVALACL
                     AVIMIPAARDTFHGHTNGSTYYNAAFGGFGAMGRLGMQREFWGNTAFSALPWLNEHAR
                     KNAQIDFHDTAWDSVRMYWRDGLLRRDIKPLWDSERADYFLFHWHKEFLDLEDRSRKA
                     MGDEVPVHVVEQDGVPLLNVYQRTERKPITSLPVVRPVGDPGGDE"
     gene            2344..3222
                     /locus_tag="PKH54_00985"
     CDS             2344..3222
                     /locus_tag="PKH54_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98487.1"
                     /translation="MKTPRKTMIAGFAIPLLSILFFGVSLRQTARVPSPADWEAAAAI
                     VRQGWQDGDVVAFMPQWAQGASPWLRGLRVDTGENHDWYELSKAARVWLISSMNGRGD
                     LPPEGWTMIDSRLMDKVDVSTWSPPRDRTLAFDFRGGIDKAVVSRTRGKTTQFCSNFK
                     KNRWFCGQEHPWLYVGQESRDIAGRVRDIIWAHAVDRAVVEALWREVPRGTTLVVHYG
                     LTQRAAEAREGSETVFSVLIDGKQVFSDTLKNDRHGWFRREFKLPGDGTSSVAFRVQA
                     DDPQSRQLCFTADAWK"
     gene            3327..4181
                     /locus_tag="PKH54_00990"
     CDS             3327..4181
                     /locus_tag="PKH54_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98488.1"
                     /translation="MTINRTGPIAAAAVAAMIAVLPAGFALARSYTPDLSVFQYGCDE
                     GKKCTDVAGFRDYVTQLNEALSPAYMGPAKTLGYRGFEITYSVGFTPVGSGGAYWSDR
                     QDGQPGLEDNPGNHYFTNQLRIRKGLPYSIQLGGSVTHMFESSLWGIGLDVSWAFVEG
                     YRKAPDVSLAVSVGTVLGAADLLNVNLNAALIISKSIAIAGLFSIEPYAGYNLMFVSA
                     GTHLVPGWDPSGYGEPFAVGPEYILRHRLEAGMNVVVENFMFGGTMMLDFQSVRVGGS
                     VRVGVRFW"
     gene            complement(4168..5499)
                     /locus_tag="PKH54_00995"
     CDS             complement(4168..5499)
                     /locus_tag="PKH54_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98489.1"
                     /translation="MDPLPQPVLVREAFTVPGLFEPGSHRFVDGGIEVFGRLQVDGVR
                     IPIGIQAGHPDSSGGWPFSGESASLCWWPLRSAAPLPSGAGGAVESLMRRLIPALPQN
                     GWLEGTLSHRRLPSRTWAFELPGGTRTIEPDLLEAVHGGNHIDLYVQLHSNCVQDCVF
                     CPTCSPDFTPDQQDADVDFIRDLAERVVIPAARFGVTSSFRFDADDVSAHRRLPALMK
                     IVSESCGCPMHLVIPANRLSVPAVADAVCGLPGLMKLSTTLFGATPETHDRVAGRAGA
                     FSETIRAIRNLSGRPLWVQGHFILSSAACAEIGAVADTLAHFGVDLMIQSLIADCDAH
                     EVVLRPIMPDLAEVRRQFAASAPRILAASSIINVAITDFPACVVPVELGHLAQTDHRR
                     ETLYRYAIPPACQGCACIDQCIGVSPMYLELFGDAAFSPLTEDGCTRATRT"
     gene            complement(5517..6893)
                     /locus_tag="PKH54_01000"
     CDS             complement(5517..6893)
                     /locus_tag="PKH54_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOC98490.1"
                     /translation="MIYRPEEISETIRMVEAEHLDIRTVTMGICMLGCPGKDPETVYK
                     YIYNMVTSNAGRLVDCAAEVENMYGIPIVNKRVSVTPMALVLEGAPRESFLAAAQALE
                     RAAVECRIDYIAGFSALVQKGATSGSAMLMRSLPEVLAATTRVCCSVNLASSRTGINL
                     EATAIMGEVMSETAHRTKHGAGCARLVVFANAPEDNPFIAGAMHGVGEPDVVINVGVS
                     GPGVVLAAIERQKRLESSLMDCQTLSEIIKRTAFKITRAGELIGREVVARLGPPVRFG
                     VLDLSLAPTPAEGDSVAAIIEAMGIERVGAHGTTAALYLLTEAVKKGGVMASSSVGGL
                     SGAFIPISEDAGMVDATRLGIMTLDKLEAMTAICSVGMDMVALPGDTPPSTIAAIIAD
                     ELAIGVINHKTTACRLIPIPGAKPGDTVDLGGLLGELIVMDTKKCDVSTFIARGGRIP
                     APMNSLRN"
     gene            complement(6943..7572)
                     /locus_tag="PKH54_01005"
     CDS             complement(6943..7572)
                     /locus_tag="PKH54_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC98491.1"
                     /translation="MGKRIVTEKDVEGLASRGVIEVDSGMIVTPLAREFAMRQGIRLV
                     YAEGSVPSAESVPGDPGSGSRGPNDDRLRKVIAEEVVRALGSSSDAGRRFIPSYSPLA
                     EDDAAQKIAAHRAGEPNRAIVVATGRNQPGVAAALTSAISDCGTDIQDISQTIVSNFF
                     SMIFVVNLDTLKGGMNFKEFKASVEQAGRGVGAEIMVMHEAILKAMHRI"
     gene            complement(7593..7940)
                     /locus_tag="PKH54_01010"
     CDS             complement(7593..7940)
                     /locus_tag="PKH54_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC98492.1"
                     /translation="MARVVGCVWSSVKWKGLSGLRMLLVRPYTLTDLKAAGGLVADQG
                     VAPSGLSDGVVVADLLGAGPGEDVIVAYGHAGRVGLEPDLPPGVAPGFPIDASVVAIV
                     DRFRVDESATAAG"
     gene            complement(8014..9933)
                     /locus_tag="PKH54_01015"
     CDS             complement(8014..9933)
                     /locus_tag="PKH54_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98493.1"
                     /translation="MDRSLPAWGIYRLLKALGDKKATGLLNVQFEGGRTRFIFKQGFV
                     TDCHTDIVTLSFGTYLNESGLVPDRDRALSMTGVEQLVSERVVRAEDSVRLHTNYVRS
                     VLGVLMPEPVLGWSFAPSLSPESAGTEEAVDVCAELFTVVQTYHDTSMMRQVVERFGP
                     AVRVTQSVSPACVMHARAHFKSSLLVSQLLAGTCTVINPDILDDDRSLRILFAFAVAG
                     CLGACTPEDAVFAPPASGSRPGRRNTREARAHAAAAAGQVSPARPSPAADARPEFAWP
                     EFQEVSRRLSEDAGGGHLSPPGGARVPQQVSPVAPPPPSAVSPAVASSVSNGLDFVTS
                     GDADEPPAVDPQSPTLDVDGDVPDVVDTAFDGFGDAPRKAPVKVRKKIDGKPNPIDPY
                     LEDALNAETQRAAGLNHYELLEINADARLSSVRMAVLRARRRFSVSRYEGSVSADALL
                     RLHGLLDRIEKANDVLNNLDARVAYNRSLGISSPALESVLVAMFDAREMWRQGTALLD
                     GRKINEALEKFENAESYDAEEPLYLCSQGMAMLALPPADDTNETIEGLIDVAFSFDQD
                     LVEAHILAAHYMVRRNDHEAARTHIRKVLAIDPDNRDARSFKQKSRVSQVGAQVSFKK
                     KQESLLDKALKLIKR"
     gene            complement(10056..10826)
                     /locus_tag="PKH54_01020"
     CDS             complement(10056..10826)
                     /locus_tag="PKH54_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOC98494.1"
                     /translation="MIPTRLIEDIEPKLIARFIDRKALFLQHWRFRKGGAGQPQWNHR
                     IRVELEPMLAARMEAGAGAGVWRPRAVLAVLPTWRVGDSLILGDGEGGRGNEVMRVRF
                     VRNDRGSCIADVVPTMEESGGDVHRTAWFACTLGPGAANHEARLRAAGEFEEYHLFHG
                     LSVALAEALAEEVHRMARIELGIPDPPELTAADVVAGKYRGRRFSFGYPCCPDLELQV
                     RLLEILGAARIGIAVNETFQMEPELSVTAGVISGCVEF"
     gene            complement(10851..13397)
                     /locus_tag="PKH54_01025"
     CDS             complement(10851..13397)
                     /locus_tag="PKH54_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC98495.1"
                     /translation="MRNSSPVLEAAASRVVVIDGAMGTEIQKVPLTSADFGGTDGLNE
                     MLVLSRPDVITRIHESFLEAGADIIETDSFSANRIVLAEYGIPEMAASISQAAASLAR
                     AAADRWSVPGRPRFVSGSVGPGSRLPSLGQVEPEEMQDAYREQAVGLLDGGVDLFQVE
                     TCQDPLQCRLALMGLAQAMELRGRHVPIFCQVTVDRAGRMLGGTDVTAAFATVSAMPG
                     VSVFGLNCSTGPDAMLPVLSRLSRTAWLPLAALPNAGLPENDGGRLVYRLSPADFAAQ
                     ARAFVIDCGVAFVGGCCGTTPEHIRELASAVDGLVPRMPTESDLSNGPGVVSSLFSAV
                     PLIQEPGPLIIGEATNANGSKAFRDYLVSGDLEGMLAVGRGQVEEGAHILDVCLAVAG
                     RDEAADVRAFVPLAGRLLDAPLMIDSTEPAVIQAALMRCPGRAVVNSVNLEDGGARLD
                     EIARIARRHGAAVVGLTIDESGMAMTAAGKFDVACRIVERLRFHGIQDRDILLDPLTF
                     TMASGDASLRSSAVETIEAVRMIKSGIPKVLTSLGVSNCSYGLKPPARRVLNSVMLHH
                     CMQAGLDAAIINSRKILPLSSIPVEMRQAASDLILDRRTSGRDPLQAFIEMFEVGISM
                     FSAETTAGEACRDPVERCRRAVIRGERAGLDEVILELAGRMGPLDVINGVLLDAMRQV
                     GDLFGRGEMQLPFVLRSAAVMKDAVQILEPLLATDSRSVRGTLVLATVAGDVHDIGKN
                     LVDIIISNNGYRVVNLGIRQTADEIMQAAVREKADAVGMSGLLVRSTLAMKDNLVAMD
                     RAGFSIPVLVGGAALDIGFVREQLKPSYRGHVEYCRDAFEGLKALDALLG"
     gene            13572..>14348
                     /locus_tag="PKH54_01030"
     CDS             13572..>14348
                     /locus_tag="PKH54_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98496.1"
                     /translation="MFRLSPSARIAFAQLAQMIVFFAAIIFILGSTSRLFDKTAFLSE
                     RLEPSVEKLNRIDEQLSTATALLESARQEDLRRADRMLVRNRPIRHIRTLATQEFPAL
                     AASPDIDRETAARLLSAADFFRTAPAPGTAAAMDSEVEIDGLRIKLTGAMQDPPKARI
                     VALELAARLQAIRTSVRNASRQTAQVTTDARHELVSRRSTLWTSILAVSIGSLILSIV
                     FLIAAIRSTRPIGDITLAVRRLAAGDLSHVKIRASRELEET"
BASE COUNT         2842 a         4570 c         4288 g         2648 t
ORIGIN      
        1 cttcccgcgg agatgaagaa cggtttcgcc acggatgcga cgctggctct tcagtctgcc
       61 ttcatcaaca gggcgacgaa aaagttccag gaaacccttg cgacggcctt cgcagaaaag
      121 ggctggttcg acagcgagac gtcaaatatc attttccgaa accagttcgt gatcccctcg
      181 aaggcggagg atcccgaatt caacccggat tctccggggt tctcggccgc gctgcgaaag
      241 ggggcatgcc tcccgttgca cgcgatggtt tcggaggccg tatccctgtc cgcgccgatg
      301 ctcggattga aagggatcga caacgccggg ctgaacctgg tgttccagga tctttacaag
      361 aaggccgttg ccggcttcgc ccagtacggc ggggagaagc gcgccccgaa cggccagccc
      421 tacctgtgca tgatccaggt tcctatgaag gatttcgtgg cttccctctt caggtttgcg
      481 gaatgacaac cggccggcac aggggctgac cgtgacaaac agactcagaa cattcgactt
      541 cgtggctggc cttttccttg cgggctgtta cttcgcgatg cttgtcggca cgaccgacat
      601 cggcttctcc cgcgacgaag ggttctattt caaggcggcc gaggaatact cctggtggta
      661 cgacgaactg cttgacgacc cgtccagtgc cttcacgaag aagtcaatcg atcgtcactt
      721 cgcatacaac ccggagcacc cggcccttcc aaagatgctc tacggcctct cctggcgcct
      781 tttcggaaag atgcatgacc cgaaaacgga accctgggcc cgggtctggt atcacaattc
      841 aagcccgccg aagccgatcc tcggcatcat gaaggaatcg acggcgatgc ggctgcccgc
      901 gctgctgatc aactccctgc tcgtcatgtt catctggctt ttcgcggccg aatacttcag
      961 ccgtcgcacg gcccttgtcg catccctttc ctggatgttc ctcccgcacg ccttctggca
     1021 ttcccacctg gcgtgcttcg acatgccggt cgtggcgatg tggttcctgg ttgcgtggct
     1081 gttccttcgg gcaacgccgc cccttgaaga acgtctcagg aaccggcgtc aaacctggtt
     1141 cagggcgatc ctggccggcg tcgcctgggg gctggcgatg tcaacaaagc acaatgcctg
     1201 gttcataccg gccgttttcc ttgcgtggta catgatcgcc aggtacgacg aagtgctgcc
     1261 gagaaacggg atcggtcgca gaagacttgc cttcgggaac atccctcccg ctttctgggc
     1321 gatgctcgtg cttggcccgc ttgtcctttt ccttacatgg cccaagctct ggttcgatcc
     1381 catcgaacac ctgaaattct atatagccag gcacgcgaag cacgaattct actgggcgta
     1441 tttcttcggc ttccttcacg tgaagccgcc gttcccgatt tcgttcccgt tcgtcatgtc
     1501 ggcaatgacg ataccggttc ccgtcatcct gctttccctg acgggcctgg caagagcctt
     1561 ccgggaatgg atccgtcgca gggccgacag gttccaactc atcaggcgtc cgcttccggt
     1621 tcggggcaac gacaccatcc ttttcgtttt cctgaacttc ctctttccgt tcctgatcat
     1681 cgcggcgccg aacacgccga ttttcggggg aaccaagcac tggatgcccg gcgtcgcgtt
     1741 cctggcgatt tttgccggcc tgggattcga gacggtactc gacgcaatcc gcgggctcgg
     1801 ggaaaggttc gcggccttta atcgccggac ggtgcggttc gcggtggcgc tcgcctgcct
     1861 ggccgtcatc atgattccgg cggcgcggga cacattccac ggccacacca acggaagcac
     1921 ctactacaat gcggctttcg gcggattcgg ggcaatgggg cgcctgggga tgcaacgcga
     1981 attctggggc aacaccgctt tttcggccct gccgtggctg aacgagcatg ctcggaagaa
     2041 cgcgcagatc gacttccacg acacggcatg ggactcggtt cggatgtact ggcgggacgg
     2101 gctgctccgc cgtgatatca agccattgtg ggacagcgag cgtgccgact atttcctttt
     2161 ccactggcac aaggaattcc tggatcttga agatcgctca aggaaggcca tgggggatga
     2221 agttccggtt cacgtggtgg aacaggacgg cgttcctctt ctgaacgttt atcagcggac
     2281 ggaaaggaag ccgatcacaa gccttccggt tgtgcgtccg gtcggcgacc cggggggtga
     2341 cgaatgaaga ctccccggaa gaccatgatc gcgggatttg caatccccct gctttcgatt
     2401 cttttcttcg gggtatcgct gcggcagacc gcgcgggttc cgtccccggc cgactgggag
     2461 gcggcggccg ccatcgtgcg ccagggctgg caggacggcg atgtggtggc gttcatgccc
     2521 cagtgggctc aaggcgcctc cccctggttg cggggccttc gcgtcgatac cggcgagaat
     2581 cacgactggt acgaattatc caaggctgca agggtctggc tgatctcctc gatgaacgga
     2641 aggggcgacc tgccgccgga aggctggacg atgatcgatt cacgcctgat ggacaaagtc
     2701 gatgtcagca catggagccc cccgcgggac aggactctgg cgttcgactt caggggcggc
     2761 atcgacaagg ccgtcgtatc ccgcacccgg ggaaagacaa cccagttctg ctcgaatttc
     2821 aagaaaaacc gctggttctg cggccaggaa cacccatggc tgtacgtcgg ccaggaatcc
     2881 agggatatcg ccggaagggt tcgggacatc atctgggccc atgccgttga ccgggcggtg
     2941 gtcgaggcgc tctggcgcga ggtaccccgg ggaaccacgc ttgtcgtcca ttacggcctg
     3001 acccagcgcg ccgccgaggc cagggaagga tcggagacgg tattttcggt gctgatcgac
     3061 ggcaagcagg tcttttccga caccctgaag aacgacaggc atggctggtt caggcgcgaa
     3121 ttcaagcttc ccggcgacgg tacgtcttcc gtggctttcc gggtgcaggc ggacgacccc
     3181 cagtcgagac agctctgctt cacggccgac gcgtggaaat gattggcccc gactcccccg
     3241 accgctgact ttttctgaat tcccggcgaa tatcggctag aatgtgcatc ggcgtccggt
     3301 cgtccgaatc tgcggggatt ggaaagatga ccatcaacag aacggggccc attgccgccg
     3361 cggccgttgc cgccatgatt gcggttctcc cggcgggctt tgcccttgcc cgaagctaca
     3421 ccccggatct ttccgttttc cagtacggct gcgacgaagg caagaaatgc accgacgtcg
     3481 ccggcttcag ggactatgtc acgcagttga acgaagccct ttcgcccgcg tatatggggc
     3541 cggccaagac cctcgggtac cgcggatttg aaatcaccta ttccgtcggc ttcacgcccg
     3601 tgggatccgg cggggcttac tggagcgacc ggcaggacgg gcagcccggc ctcgaggaca
     3661 atcccggcaa ccattacttc accaaccagt tgaggatccg gaagggcctt ccctacagca
     3721 tccagcttgg cggttctgta acccacatgt tcgagagttc cctgtgggga atcggcctgg
     3781 acgtttcctg ggccttcgtc gaaggttacc gcaaggcgcc cgacgtctcg ctggcggttt
     3841 cggtcggcac ggttctgggc gcggctgacc tgctcaacgt gaacttgaac gcggcgctga
     3901 tcatttccaa atcgattgcg atcgcgggcc tgttttccat cgagccttac gccggctaca
     3961 acctgatgtt cgtgtctgcc gggacgcacc ttgtgcccgg atgggatccc tcgggatatg
     4021 gcgagccttt cgccgtcggc cccgaataca tcctgcgcca ccgcctggag gctggcatga
     4081 acgtcgtggt cgagaacttc atgttcggcg ggacgatgat gcttgatttc cagtcagtaa
     4141 gggtcggcgg ttccgtacgc gtcggggtca ggttctggta gcccgcgtgc agccatcttc
     4201 cgtcagcggc gaaaatgccg cgtcaccgaa aagttcaaga tacatcgggg acacgccgat
     4261 acactgatcg atgcaggcgc agccttggca tgccggggga atcgcgtacc tgtaaagcgt
     4321 ttcccgtcga tgatcggtct gggcaagatg accgagttcc acgggcacga cgcacgccgg
     4381 aaaatcggta atggcgacgt ttatgatgct gctcgcggca agaatgcgcg gggcagaggc
     4441 cgcaaactgg cgccttacct cggcgaggtc cggcatgatg gggcgcagca cgacttcgtg
     4501 agcgtcgcag tccgcaataa gcgactgaat catgagatcg acgccgaaat gggccagggt
     4561 atcggcaacg gcgccaatct ccgcacaggc ggcggacgaa aggatgaaat ggccctgaac
     4621 ccagagcggc ctgccggaaa ggttcctgat tgcccggatc gtttcggaaa aagcccccgc
     4681 gcggccggcc accctgtcgt gcgtttccgg cgtggccccg aacagggttg ttgaaagctt
     4741 catcagtccg ggaagaccac aaacggcgtc cgccaccgcg ggcacagaca gccggttggc
     4801 cgggatcacc aggtgcatcg gacaaccgca cgactcggag acaatcttca tcaaggcggg
     4861 aagacgccga tgcgccgaca cgtcgtccgc gtcgaacctg aacgaggatg tgacgccgaa
     4921 ccgggcagcc gggatgacga cccgctcggc cagatcgcgg atgaaatcaa cgtcggcatc
     4981 ctgctgatcc ggcgtgaaat ccgggctgca cgtcggacag aatacacagt cctgaacgca
     5041 gttggaatga agctgcacat aaagatcgat gtggttgccg ccatgaacgg cttcgagaag
     5101 atccggctcg atcgtccttg tgccaccggg cagttcgaaa gcccaggttc gcgaaggaag
     5161 cctgcgatga ctcagcgtac cttccagcca gccgttctgc ggaagcgccg ggatcaaccg
     5221 ccgcatcagc gattcgacgg cgcctccagc gcccgatggc aacggggccg ccgacctcag
     5281 gggccaccag cacagggatg ccgactctcc ggaaaaaggc caaccgccgg aagaatcggg
     5341 gtggccggcc tggatgccga tcgggatccg gactccatcg acctgcaggc gcccgaacac
     5401 ctcgatgccg ccatcgacaa acctgtggct tccaggttcg aacaacccgg gaacggtgaa
     5461 cgcttccctg acgagaaccg gctgcggcag aggatccata caccccccgg cgatgttcag
     5521 ttgcgcaagg aattcatcgg cgcgggaatt cgaccgccgc gcgcgatgaa agtgctgacg
     5581 tcacatttct tggtgtccat gacgatcagt tcgccaagca gtccacccag atcgaccgtg
     5641 tcgccgggct tggcgcccgg tatcggaatc aggcggcagg cggtggtctt gtggttgatc
     5701 acgccaatcg ccagttcgtc cgcgatgatg gccgcaatgg tcgatggcgg ggtgtcgccc
     5761 ggcagcgcga ccatgtccat gccgaccgaa cagatcgcgg tcatggcctc cagcttgtcc
     5821 agtgtcatga ttccgagcct ggtcgcatcg accatcccgg cgtcctcgct gatcgggata
     5881 aaggcgcctg aaaggccgcc gaccgaggac gacgccatca cgccgccctt cttgaccgct
     5941 tcggtaagca gataaagcgc ggcggtcgtc ccgtgggctc caaccctttc gatgcccatg
     6001 gcctcgatga tggccgcaac cgaatcccct tcggccggag tcggcgcaag tgacagatcg
     6061 agcacgccga atcggactgg cggtccaagc ctcgcgacca cctcgcgacc gatcagttcg
     6121 ccggccctgg tgatcttgaa ggctgtacgc ttgatgattt ccgaaagcgt ctggcagtcc
     6181 atcagggacg actcaagacg cttctgccgt tcaatggcgg cgagaacgac ccccggcccc
     6241 gaaacgccga cattgatgac gacgtcgggc tcgccgacgc cgtgcatggc accggcgatg
     6301 aaggggttat cctcgggcgc gttggcgaat acgaccagcc tggcgcaacc ggcgccatgc
     6361 ttcgtccgat gggccgtctc gctcatgacc tcgcccatga tggccgtcgc ttccaggttg
     6421 atgccggttc tcgaggacgc aaggttgacg ctgcagcaga ccctggtggt cgcggccagc
     6481 acttccggaa ggctgcgcat caacatcgcc gaaccggacg tggcaccctt ctggaccagg
     6541 gccgaaaatc cggcgatgta gtcgatcctg cactccacgg cagcccgctc aagtgcctgg
     6601 gccgccgcca ggaacgattc ccggggagcc ccctcgagca ccagggccat cggcgtgacc
     6661 gaaacgcgct tgttgacgat ggggatgccg tacatgttct cgacctcggc cgcgcaatcg
     6721 acaagccgac cggcgttcga ggtcaccatg ttgtaaatgt acttatagac ggtctcggga
     6781 tccttgccgg gacagcccag catgcagatt cccatcgtga ccgtccgaat gtcgagatgc
     6841 tccgcctcga ccatccttat cgtttccgat atctcttccg gcctgtaaat caactgaacc
     6901 tccggatcgc ggccgccgcg gacgcgtgct ccgccacgaa catcagatcc tgtgcatcgc
     6961 cttaaggata gcttcgtgca ttaccatgat ttcagcgccg acgccgcgac cggcctgctc
     7021 gacactcgcc ttgaactcct tgaagttcat tccgcccttc aaggtgtcca ggttgacgac
     7081 aaaaatcatc gaaaagaaat tcgagacgat cgtctggctg atgtcctgga tgtcggtgcc
     7141 gcagtcggat attgccgacg tcagggccgc ggccacgccg ggctggttgc ggccggtcgc
     7201 caccacgatg gcccggttcg gctcaccggc gcgatgggcg gcaatcttct gtgccgcgtc
     7261 atcctcggcc aggggtgaat acgaaggaat gaacctgcga cccgcatcgg aggaagatcc
     7321 cagggcacgg acgacttcct cggcaatcac cttgcgcagc cgatcgtcgt tcggcccgcg
     7381 gctcccgctt ccgggatccc ccggaaccga ctcggccgac ggaacggagc cttcggcata
     7441 aacaagccgg ataccctgcc tcatcgcgaa ttcacgcgca aggggggtta cgatcatgcc
     7501 ggaatcgacc tcaatgacgc ccctggaggc aaggccctcg acatcctttt cggtgacaat
     7561 tctttttccc acggcaacct cttgaaatgg gatcacccgg cggccgtcgc cgactcatcg
     7621 acgcggaacc ggtcaactat tgcgacgacg gatgcgtcga tcgggaagcc cggggcgacg
     7681 cccggtggca ggtccggctc aagtccgacc cttccggcgt gcccgtacgc gacaatcacg
     7741 tcctcgccgg gtcccgcgcc cagcagatca gcgaccacga ccccgtcgga aagcccggag
     7801 ggagcaaccc cctggtcggc gacaaggccg ccggccgcct tcaggtccgt caacgtgtat
     7861 ggcctgacca gaagcatcct cagccctgac agccccttcc acttgaccga agaccagacg
     7921 caaccaacaa ccctggccat gaacatggct tcctccggaa accggaaatc agacgggaat
     7981 ttcgcctttt ttcagtgaag tgtcaatcgg gcatcaacgc ttgatcagct tcagcgcctt
     8041 gtccagaagc gactcctgct tcttcttgaa gcttacctgg gcgccgacct ggcttacccg
     8101 cgacttctgc ttgaacgacc tggcatccct gttgtcggga tcgatcgcca gaaccttcct
     8161 gatgtgggtt ctggccgcct cgtgatcgtt tcgcctgacc atgtagtggg ctgccaggat
     8221 gtgggcctcg acaagatcct ggtcaaaact gaaggccacg tcgatcagcc cctcgatggt
     8281 ttcgttggtg tcgtcggccg gcggcagggc cagcatcgcc atcccctggg aacagaggta
     8341 aagcggctcc tcggcatcgt aactttcggc gttttcgaac ttctcgagcg cctcgttgat
     8401 ttttcggccg tcaagaagcg cggtgccctg tcgccacatc tccctcgcat caaacatcgc
     8461 gaccagaacc gattccagcg ccgggctgga tatgcccagc gaacggttat aggccacgcg
     8521 cgcgtcgaga ttgttaagaa catcgttcgc cttctcgatc ctgtcgagca gcccgtgaag
     8581 acgcaggagc gcatcggcac ttacggaacc ctcgtacctg gacaccgaga aacggcgacg
     8641 cgcgcgaaga acggccatcc gaaccgagga cagcctggca tccgcgttga tttcaagcag
     8701 ttcgtaatga ttaaggccgg ccgcgcgctg ggtttcggcg ttcagggcgt cctcgagata
     8761 cggatcaatc gggttcggct tgccgtcgat cttctttctt accttgacag gcgcctttcg
     8821 aggggcgtcc ccgaacccgt caaaagcggt atcgacaaca tcggggacat cgccatcaac
     8881 gtcaagggtc ggggactgcg ggtcgactgc cgggggctcg tcggcatcac ctgaagtaac
     8941 gaaatcaagg ccattggata ccgaagacgc aacagcaggg gagactgctg aaggaggtgg
     9001 tggtgcaacc ggcgagacct gctgcggaac cctggcaccc cctggcggcg aaagatgtcc
     9061 gcccccggca tcctcggaaa gccgcctgga aacctcctgg aactcgggcc acgcgaactc
     9121 cggcctggca tcggcggcag gcgacggcct ggccggggac acctgaccgg cagcggcggc
     9181 ggcatgggcc ctggcctccc gcgtgttccg tctgcccggc ctggaaccgg aagcaggtgg
     9241 cgcgaaaacg gcgtcctcgg gagtacatgc gccaagacag ccggcgacgg caaaggcaaa
     9301 caatatccgc agacttctat catcgtccag gatatcggga ttgattaccg tgcacgtgcc
     9361 agcaagaagc tgggaaacca gcagcgaact cttgaaatga gccctggcgt gcatgacgca
     9421 cgcagggctg acggactggg tcaccctgac ggcaggcccg aacctctcga cgacctgacg
     9481 catcatcgat gtgtcgtgat atgtctgaac aaccgtaaaa agctcggcac agacatcgac
     9541 ggcctcctcg gtgccggcag actccgggga aagcgacggg gcaaaggacc atcccaggac
     9601 gggctccggc atcaacacgc ccagaacgct gcgaacatag ttggtgtgaa gcctgacgct
     9661 gtcttcggcc ctgacgaccc tttccgacac gagctgttcg acgccggtca tggaaagggc
     9721 cctgtcgcgg tcgggcacaa ggccggattc gttaagatag gtgccgaacg agagtgtgac
     9781 gatgtccgtg tgacaatcgg tcacgaagcc ctgcttgaag atgaaccggg ttctgccgcc
     9841 ctcgaactgc acgttcaaaa gcccggtggc cttcttgtca ccgagggcct tcagaagccg
     9901 gtaaattccc caggctggaa gcgacctgtc cacggcatct cctgtcagtc ccgaaccgat
     9961 ccgaaggcgc gggaacaaga aacgtcccga tccgcgcaat gcagaagcac ctgccgaacc
    10021 ggtcgcgcac agtataacag agttgtaaag gaatgctaaa actcgacgca accggaaatg
    10081 accccggccg tgaccgacag ttcgggctcc atctgaaaag tttcgttgac ggctatcccg
    10141 atgcgcgcgg caccaagaat ttccagcagc ctgacctgaa gctcaagatc cggacaacat
    10201 gggtaaccga acgaaaagcg tctgccacga tacttgcccg cgacgacgtc cgcggcggtc
    10261 agttcgggcg gatccggtat tccaagctcg atccgggcca ttctgtgcac ttcctcggcc
    10321 agggcctcgg ccagcgcaac cgacagcccg tgaaaaagat ggtattcctc gaattcaccc
    10381 gcggccctga gcctggcttc gtgattcgcg gcccccggtc caagcgtgca ggcgaaccat
    10441 gccgtccggt gaacgtcgcc gcctgactct tccatggtcg gcaccacatc ggcgatgcag
    10501 cttccgcgat cgtttcggac aaacctgact cgcatgacct catttccccg gcccccctcc
    10561 ccatcgccca gaatcagcga atcgccgacc cgccatgtcg gcaggactgc aaggacggcc
    10621 ctcgggcgcc agacacccgc accggccccc gcttccatcc tcgccgccag catcggctcc
    10681 agttcgacac ggatgcggtg gttccactgg ggctgacctg ccccgccctt cctgaaacgc
    10741 cagtgctgaa gaaaaagcgc cttgcgatcg atgaagcgcg caatcagttt cggttcgatg
    10801 tcttcaatca gccttgtcgg aatcattact tccccgcagt ccgggcaacg tcaaccgagc
    10861 agggcgtcaa gcgccttcaa gccttcgaag gcgtctcggc agtactcgac atgtccgcga
    10921 tatgacggct tcaactgttc gcggacgaaa ccgatatcga gcgcggcgcc gccgacaagc
    10981 accgggatgc tgaagccggc cctgtccatg gctacaagat tatccttcat cgcaagcgtt
    11041 gaacggacca gcagaccgct catgccgacc gcgtcggcct tctcgcggac ggccgcctgc
    11101 atgatctcgt ccgccgtctg ccggattccc aggtttacca cccgataccc gttgttcgaa
    11161 atgattatgt cgacaagatt cttgcctatg tcgtggacgt ccccggccac cgtcgccagc
    11221 accagggttc cccggacgga acgcgaatcg gttgcgagca gtggttcaag gatctgaacc
    11281 gcgtccttca tgaccgccgc cgaacgcagc acgaaaggaa gctgcatttc accgcggccg
    11341 aacagatccc cgacctggcg catggcgtcc agcagcacgc cgttgatgac atccagcggc
    11401 cccatccggc cggcaagttc aaggatcacc tcgtccagcc cggcgcgctc gcccctgatg
    11461 accgccctgc ggcagcgctc gacgggatcc cggcacgcct cgcccgcggt cgtttccgca
    11521 gaaaacatgg atatcccgac ctcgaacatc tcgatgaacg cctggagagg atcccgaccg
    11581 gatgtccggc gatccaggat caggtcggat gcggcctggc gcatctcgac cggaatcgag
    11641 gacaacggaa gaatcttgcg cgaattgatg atcgcggcat ccagcccggc ctgcatgcaa
    11701 tggtgaagca tcacgctgtt aagaacgcgt ctcgccgggg gcttcaaacc gtatgagcag
    11761 ttgctgacgc caaggcttgt aagcaccttt gggattccgg acttgatcat ccggacggcc
    11821 tctattgtct cgaccgcgga cgatcgcagc gaggcatcgc cggaggccat cgtgaaggtc
    11881 aacggatcca ggaggatgtc gcgatcctgg atgccatgga aacgcaatcg ttcgacgatg
    11941 cggcaggcga catcgaactt ccccgccgcg gtcatggcca tgcccgattc atcaatcgtc
    12001 aggccaacga ccgccgcgcc atgacgcctg gcaatccggg caatctcgtc gagcctggcc
    12061 ccgccgtcct cgagattgac cgaattgacg acggcacggc cgggacaacg catcaatgcg
    12121 gcctggatga ccgccggctc ggtcgagtca atcatcaagg gggcatcaag cagccttccg
    12181 gccagtggaa cgaacgccct gacgtccgcg gcctcatccc gcccggcaac cgcaagacag
    12241 acgtcaagaa tgtgggcacc ctcttccacc tgtccgcgcc cgacggcaag cattccctcc
    12301 agatccccgc ttacaaggta atccctgaag gccttgctgc cgtttgcgtt cgtcgcctcg
    12361 ccgattatca gggggcctgg ttcctggatc agcggcacgg cggaaaaaag ggatgatacg
    12421 acccccggcc cattggacag atccgattcg gtcggcatgc gcggaacaag gccatcgacc
    12481 gcgctggcaa gctcgcggat gtgttccggc gtggtgccgc agcatccgcc aacgaacgcg
    12541 accccgcaat cgatgacgaa ggccctggcc tgagccgcga aatccgccgg agacagtcga
    12601 tatacaagcc ttcctccatc gttttccgga aggccggcgt tagggagtgc cgcaagcgga
    12661 agccaggcag tccttgaaag cctggaaagc accggaagca tcgcgtcggg gccggttgaa
    12721 cagttcaatc cgaaaacgga cacgccgggc atggccgaca cggttgcaaa tgcggccgtc
    12781 acgtctgttc cgccgagcat ccgcccggca cggtcaaccg tcacctggca gaaaatcggg
    12841 acgtggcggc cgcgaagttc catggcctgc gccagcccca tcagtgccag cctgcactgc
    12901 aacggatcct ggcacgtttc cacctggaaa aggtcaaccc cgccgtccag aagtccgacc
    12961 gcctgttcgc gataggcatc ctgcatttcc tccggctcga cctggccaag ggacgggagg
    13021 cgggatccgg gaccgacaga acctgaaaca aaccggggcc ttcccggaac cgaccacctg
    13081 tccgcggcag cccgggcaag cgaggcagcg gcctgggaaa tcgacgccgc catctcggga
    13141 atgccgtatt cggccagaac gatgcgattt gccgaaaaag aatcggtctc gatgatatcc
    13201 gcaccggcct caaggaacga ttcgtgaatt cgagtgatga catccggccg cgaaaggaca
    13261 agcatctcgt tcaggccgtc agtgcctccg aaatcagcgg acgtcaacgg caccttctgg
    13321 atttcggtgc ccatcgcgcc atcgatcacg acgacccttg acgcggcggc ctcaaggact
    13381 ggactgctgt ttctcatgaa aaggcccctt ttcggttcaa cactgacctg ccggcaagga
    13441 taacgccaaa atgcgcggat tggctaaaca tcgacagcgg ccggagggcg cggtaaagtg
    13501 tcgcgtacat ctttcccggg gacgccgacc cggcgtggcg gcgcaacctg ttcagggaat
    13561 gcggaggagt cgtgttccgg ctttcgccgt cggcaagaat cgcgttcgcg cagcttgcgc
    13621 agatgatcgt ctttttcgcg gccatcatat tcattctcgg aagcacttcg cggctgttcg
    13681 acaagaccgc gttcctgtcg gaaagacttg agccgtccgt cgagaagctg aaccggatcg
    13741 atgaacagct ttccaccgcg acggcccttc tggaaagcgc cagacaggaa gacctgcggc
    13801 gcgcggacag gatgctggtc aggaatcggc cgatccgcca catcaggacg ctggcgacac
    13861 aggaattccc ggcacttgcc gcttcacccg acattgaccg cgaaacggcg gcccgcctcc
    13921 tttcggcagc cgatttcttc aggaccgctc cagccccggg aacggcggcc gccatggatt
    13981 ccgaggtcga gatcgacggc ctgcggatca agttgacggg ggcgatgcag gatcccccga
    14041 aggccagaat cgtggctctt gagcttgccg cgcggctgca ggccataaga acatcggttc
    14101 ggaacgcctc gcgccagacg gcccaggtga ccacggatgc cagacacgaa cttgtttcgc
    14161 ggcgatcgac actctggact tcgattctgg cggtctcgat cggatccttg atactttcga
    14221 ttgtgtttct gattgccgcc ataagatcca caaggcccat cggcgatatc acgctggcgg
    14281 tgcgcaggct cgcggcgggg gatctttccc acgttaagat acgggcttca cgggaactgg
    14341 aagaaact
//
LOCUS       DAOPPA010000015        14049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5081_length_14049_cov_3.404959, whole genome shotgun sequence.
ACCESSION   DAOPPA010000015 DAOPPA010000000
VERSION     DAOPPA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5081_length_14049_cov_3.404959"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..2916
                     /locus_tag="PKH54_01035"
     CDS             346..2916
                     /locus_tag="PKH54_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98497.1"
                     /translation="MRAGSAHDRKTIIPVAAACLAAMMAAGCGADDKGQADFLEYELW
                     PSTHIFDNDTAGQIISIDEDWTFLFRSDSEIAARLAPKHVLMAGETPLTPRGTMRRVT
                     EITPLADGIEVRTERALLQHAFKSFHTKFTREVAMEQGSLLWDTEPGAKLLDSTEHVT
                     NTTRKSGGDSVGPVSIDYYPFNGDNDISTDEDQIHVTATFSGSVYYNFGIDFDWPDVS
                     SVIGGDPLPEITVGYSVDARAESSITADGVAMYSYDRRDTLAHAPVTGFWIWILYFTV
                     SIDLMADIEGGASSLFHLETGAGASFVAAAEYSTDDGGRLIPPTADFWTLPSNVSSTE
                     SAYLKVSVGPRLRLALYDFFGPHLTVWGFGELSADMDRDPCWDLRAGVYGDIGIGVAL
                     WGETLAEWSKEFDIADTSIMSGECIPDPNAPEVPDIIDPLFDPWSKILVDTAGGVDLD
                     RDFMALQHTIDGHWLVAGSGMKTLSKVTNDGAVLWSRRYLRPDATLPIPLNMNRAINT
                     RDLGIFATAYSPVMLMKLDADGGVVRAWRPDIEFQPVVGLQVLQEDSAGNVFVGGPFV
                     KEDMGSSDAWIFKLAPDGTVLWSKIWGDPAISEWVTGFVRLDQDLVVVGESFGLAQDP
                     AHQSWAMRIGPDGQARWIKRIGGALGTESLALKKAMISSNGDIIAGGSYGLGGPDQVL
                     MKIKPDDGTLGWVNGNKAGDTSGALGIDLTDVFQLSDKGYLMTGLWWTGGTDHLWVAR
                     ADSVGRMTWLKKLETGIEAGAPGIVMTGDGGALVAAYTEIGDQDHSVWLTRLPIKTGA
                     IDLDPALASISDETFKHDQNPDLVFTTAATGLIDLPLTLLPDLVRSTRISPVETVMTK
                     "
     gene            3036..5231
                     /locus_tag="PKH54_01040"
     CDS             3036..5231
                     /locus_tag="PKH54_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC98498.1"
                     /translation="MKRASLILPIFILAGIAGSYGCGQTAGPGLDAIQDVPDEAPQDA
                     VTGDGGDAFTEEVLHDSGVDGNDIDLDLGGNELQQDQFVFVPVDDAFAPDGLHMDAND
                     ETDAFDMPDGVKVRAASYNLYGYHWSDAAHFGALIKEMNPDLMALVESNEDQVQAVAE
                     AAGYEYWCTNGDGRSLLSITPRATCETITLVGGRTLLRTETEIDGVTFAVWVMHIDWD
                     IIGNRQTRDFMDNHFVNETRSHVIMMGDFNDEHLSRQINILEEQLADAFTAYGWYPGE
                     RVTWPAQYFDDTEGSQTIDIIFFRKSLRPIVLDADAVNMSPVLSDHKPVWADMLFPRD
                     DQPFPDYMTMNRLDPYRVIWKVPGTSDNLVTNPGAEDGLAGWTVTGGGEAATERFACR
                     PAEGEKFFCGFPKGYKPDDGYTRFTQEIDLSDYIEMIDANRAWLNVSAEIITCPFLLT
                     EDDITTNHVQRYDDAEVTVTLVSADGKATDRRISKRRDTLMWYPYADAFPIPSGTRKI
                     ELALTAHKRIDYVGGIDAGFDKVYVSVREWPIHGVLDGNMLMGGIDGDSAIDGGNATV
                     SNSGWVTRTTGYPLSLNPFPPAGVSGLKYLFAGDEVGLNPLDEPAVLSQTLDLSKFGT
                     EIDKGRMTLRWGGFTRTNTAEGASVAMSLQLLDGRGDVWGEVHGRTVWESEWTRQENR
                     TRLPRGIRGARLVLRAEVPLFDGFLGIEGMETQDDAVFADELFIRPETF"
     gene            complement(5444..6265)
                     /locus_tag="PKH54_01045"
     CDS             complement(5444..6265)
                     /locus_tag="PKH54_01045"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HOC98499.1"
                     /translation="MEKRFDYKGTGAGLFGNLFVGVLLTIITLGIYLPWFVVKLYKYV
                     LENTIMKVGGSEVRFDFRGSGAELFGTWIIGVILSIITLGIYYSWFVVSVSKFFAANV
                     VARDADGREYTGTFNGTGGALFGTMFVGTLLTIITLGIYCPWFCINLARYFAGNMVIM
                     HNGSAVGTFKFDGAGGTLFGKVFVGVILSVITFGIYFSWFIVDMAKYFAQETTVEFNG
                     VVNRFRFEGTGVKLFVINLIGTILSGLTFGIYWFWFMTNQLKFKMENMVVVGPGE"
     gene            6394..8424
                     /locus_tag="PKH54_01050"
     CDS             6394..8424
                     /locus_tag="PKH54_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOC98500.1"
                     /translation="MKKGQQELSGIHFKPSLHKQLIGRLMGKPKNLREKGLFQKLALI
                     PFLAWVGLGADGLSSSSYGPMESFLALGEHWYLAIVLAVATVLTIAVISFAYGRIITK
                     FPGGGGGYVVANALLGKHAAVLAGSALTVDYVLTVTVSIAAAGDALFSFLPPGMAAGK
                     LPFEALLILALITLNLRGLREAITPLIPIFLIFLVTHAALIGGSILLHLADIPKVEEG
                     VVEGFRNGYSTLGIMGMLAIFARAWSLGGGSYTGIEAVSNGLPLLREPRVRTAHRTML
                     YMAVSLALTAGGLLIGYLLLGVRFEEGKTLNAVLSEAVFGTGTFGRALVIATLVSEGA
                     MLVVGAQAGFIGGPRILANMAMDGWVPRRFGALSSRLTSGHGILVMGVAALAALAYTG
                     GNVSTLVVMYAINVFLTFSLSMAGMLKGALFPGKGDKRNFGDAALFLTGLLICLSILI
                     ITTWEKFYQAGWLTLGVTGAITVACYLVKRHYRSIGRLIASLDRQIPRLPEIPEEPPH
                     DSGHNGRTAAILVGGYGGLGINTTREVVREFRDVFKRFVFVSVGVVDASSLQYGVEAE
                     QVLDRTKAACRQYVELASRYKIPASYRAAVATSVNDELEVICQRVAADYDDVTFFAGQ
                     LVFDRPRWYHQWLHNNTAFDLQNRLLVFGQTLVILPRLVQLGGVDTGKLPDK"
     gene            8646..10286
                     /gene="hcp"
                     /locus_tag="PKH54_01055"
                     /gene_synonym="priS"
     CDS             8646..10286
                     /gene="hcp"
                     /locus_tag="PKH54_01055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOC98501.1"
                     /translation="MFCYQCEQTAKGIGCTVAGVCGKDAVTSAMQDLLLYQCKEISFY
                     GSKARQMKLSSPAIDRFVVEGLFTTVTNVNFDADKIVEIVHRGADLLDLAIEMVNDAA
                     MANGVEPEAINRPVPNFRGSKEELLALALKTGIRERSEQRGPDATGLEELITYGLKGM
                     CAYLDHAMVLGRFSNNAVGFLMDALASLLNPQSVGDLTALALKVGEVNLEVMGLLSEA
                     NTGTYGHPVPTKVRIEPVKGKAILVSGHDLLDLEALLKQTEGKGINIYTHGEMLPCHG
                     YPGLKKYAHLVGNYGGAWQDQRKEFDEFPGAILMTTNCIQKPRETYANRIFTTGLVEF
                     PGLVHVTREDFGPVIEAALEAPGFEADGPDRTILVGFGHNAVLGVADKVIEGVKSGAI
                     KHFFLIGGCDGAKPGRNYYTDFAEKAPKDTVILTLACGKFRFNKLEFGDIGGIPRLLD
                     VGQCNDAYSAIQIAVALANAFGTDVNSLPLSFILSWYEQKAVCILLTLLHLGIRNIKL
                     GPSLPAFVTPAVLDVLVKTFNIGPVTTVEADLAAALGN"
     gene            10400..11137
                     /locus_tag="PKH54_01060"
     CDS             10400..11137
                     /locus_tag="PKH54_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC98502.1"
                     /translation="MDRNITPDNFEAWNDEMGRKFDPDAYHNHPNPLIRNIERKRVRT
                     VLKFIKPTEGQRVLEVGCGAGNVLEQIVGADLTGIDLSDHLLEKARERLRQRARLLKA
                     DAAALPFEDGWFGVVYCTEVLEHVLDPRAVIREMRRVLRPDGVAVVSIPNESVINGIK
                     GHFLDNPIGRVLLNEKPGTYHSSKKMDDEWHLHSFDLKMLKCICEGVFRIDRLAAIPS
                     PVLPIRYVARLAPIKPASHAKTAESEG"
     gene            11188..12693
                     /locus_tag="PKH54_01065"
     CDS             11188..12693
                     /locus_tag="PKH54_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOC98503.1"
                     /translation="MTAFMVALALLAAGYLLYGRLVERAVKPNPDSPVPANSCADGID
                     FVAMPTWRVFLIQLLNIAGLGPVFGAIMGALWGPQVFLWVVFGSILGGAVHDMMSGVM
                     SVRNNGASLPELIGRYLGKVPRHITTLFILILMILVGAVFVKGPAELIVQLIPAQRIG
                     DLIGGNTGELLAADWRGSSVWLWCVMAVIFAYYILATLLPVDKIIGRIYPIFGAILLI
                     MVLGLLVAMVTGRIDVVPFTLENLHPKQTPAWPTIFITVSCGAVSGFHATQSPLMARC
                     LKSETRMRAVFYGSMIVEAVIALIWAAAAAGYYGGSAGLAAAIGPGNNPAPIVMQVCK
                     GTMGTIGGILAVLGVVVLPITSGDTAFRAARLIAADYLRLPQKKILNRYIIAIPMFVI
                     SAMLNFVPFGVVWRYFGWANQTLAAVTLWAAAAYLVRRGGFWWLMAAIPATFMTVMTV
                     TFFVVSKNLSHIGMDIVPGTIVGLGFGLTSIVAFIWLMKPMGRSCHKHSSP"
     gene            complement(12682..13806)
                     /locus_tag="PKH54_01070"
     CDS             complement(12682..13806)
                     /locus_tag="PKH54_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98504.1"
                     /translation="MSRISQIAGMAAQVGLLGIEFIDLHLARLARRSPMTMPLLMFEI
                     TRRCNLSCQYCGYPRFYPDYGRELSTEEVRAILRDCVDLKTRIVSFGGGEPFMRPDFI
                     EIVAFARSLGLNVHIDSNGTMIDEEVVGAIPDGADVTIIFSLDHPDADVNDRVRGPGS
                     HAGVTRAARLLRELRPSISVGINCVVGSHNVDCLERMVELADGLGVGGISFLPLHANL
                     QHQWRFGDDSCVPLVPADPETVHRAMLAATALARSRGLHTNSRRFNSMAAGGPKSRRA
                     FQCWAGYLFGNIDPYGNLFPCYDIMDKASGVREVGLKAAWRGPGMERLRSRVRRCEAD
                     CVSSGAIEASLRMNPTIVASDPVQLIEDLRFFLGSGRSRR"
BASE COUNT         2930 a         4151 c         4151 g         2817 t
ORIGIN      
        1 gcatcgggat cgccaagccc ttcgtccagg ccggaatccg actccgagac ctggccgccg
       61 cagccggtcg ccagcatgaa cgccaccgcc aggcacaaaa ctggaaatcg ctttttcatt
      121 aatcagtcct taagaaaggt aatcatgtat tcatcccgaa cccgtgccag gtcagccgga
      181 atcgacacgc cacgggcatc cgcgccctga taccacggaa tcattgaatt tgtaaacgaa
      241 tcgggccaag cgttccggca aaatgttaag attccaaagt tcagagaacg gcgttcgatg
      301 aaacatttcc cagtcttgcg cgggccggcc cgcgggcggt gacaaatgag agccggatca
      361 gcccatgacc gtaagacgat tatcccggtg gcggccgcct gccttgctgc catgatggcg
      421 gccgggtgcg gcgccgacga caaggggcag gcagactttc ttgaatacga actttggcca
      481 tcgacccaca tcttcgacaa cgatacggcg ggtcaaatca tctccattga cgaagactgg
      541 acattcctgt tcaggtcaga cagcgaaatc gcggcccggc ttgcaccaaa gcacgtcctg
      601 atggccggcg agacgccttt gaccccgcgc ggaacgatgc ggcgggtgac cgaaataacc
      661 ccgctggcgg acggcatcga ggttcgaacg gaacgggccc ttctgcagca cgccttcaaa
      721 agcttccaca cgaaattcac gcgggaagtg gcaatggaac agggatccct tctgtgggac
      781 accgagccgg gcgcaaagct gctggactca accgagcacg tcactaacac gaccaggaaa
      841 tcgggcggcg acagcgtcgg cccggtatcg attgactatt atcctttcaa cggcgacaac
      901 gacatctcca cagacgagga tcagatccac gtcacggcca cgttctccgg atcggtctat
      961 tacaacttcg ggatcgactt cgactggccg gacgtgtcaa gtgtaatcgg cggggatccg
     1021 cttcccgaaa tcaccgtcgg ttactcggtc gatgctcgcg cggagtcatc gattacggcc
     1081 gacggcgttg caatgtattc atacgaccgg cgcgacacgc tggcccacgc gccggttacc
     1141 ggcttctgga tctggattct gtacttcacc gtcagcatcg acctgatggc cgacattgaa
     1201 ggtggggctt ccagcctgtt ccaccttgaa acgggggccg gagcatcgtt cgtggcggcg
     1261 gccgaatact cgacggacga tggtggacga ctgatcccgc caaccgccga cttctggacg
     1321 ctcccaagca acgtgtcatc aaccgaatcc gcttacctga aggtatccgt gggcccaagg
     1381 ctgcgcctgg ccctgtacga cttcttcggc ccccacctga cggtctgggg cttcggcgaa
     1441 ctgtcggccg acatggacag ggatccctgc tgggatttgc gtgccggcgt ctatggcgac
     1501 atcggcatcg gtgtcgcgct ttggggcgaa acgcttgcgg aatggagcaa ggaattcgat
     1561 atcgcggata cgtccatcat gtccggcgaa tgcatccccg atcccaacgc gccggaagtc
     1621 cccgacatta ttgacccgct gttcgacccg tggtcaaaaa tcctggtgga tacggcgggc
     1681 ggcgtggatc tcgaccgcga cttcatggcg ttgcagcaca ccatcgatgg ccactggctg
     1741 gtcgccggaa gcggcatgaa aacgctttca aaggtcacaa acgatggagc cgtgctgtgg
     1801 tcgcgcagat atctgcgacc ggatgcgacg ttgcccattc cgctgaacat gaaccgcgcg
     1861 atcaacacca gggatctggg catcttcgcg acggcctaca gtcccgtgat gcttatgaag
     1921 ctggacgcgg acggcggcgt ggttcgcgcc tggcgtcccg atatcgaatt ccagcctgtc
     1981 gtcggcttgc aggttcttca ggaggacagc gccggcaacg tgttcgtggg cgggccattc
     2041 gtcaaggagg acatggggtc aagcgacgca tggatattca agctggcgcc tgacggaacc
     2101 gtgctctggt caaagatctg gggcgatccc gcgatcagcg aatgggtgac ggggttcgtg
     2161 aggctggatc aggatctggt ggttgtcggg gaatccttcg gactggcgca ggatccggcc
     2221 caccagtcat gggcgatgcg aatcggcccg gatggccagg cacgctggat taagcggatc
     2281 ggcggcgcac tcggaacgga atcgctggcg ctgaagaagg ccatgatttc aagcaacggc
     2341 gacatcattg ccggtggttc ctatggcctt ggcggcccgg atcaggtgct gatgaagatt
     2401 aagcccgacg acggaactct gggatgggtc aacgggaaca aggccgggga cacaagcggg
     2461 gcgctgggga tcgacctgac cgacgtcttc cagctttccg acaagggtta tctgatgacg
     2521 gggctctggt ggaccggcgg caccgaccac ctgtgggtcg cgcgcgcgga ctcggtcggg
     2581 cggatgacct ggcttaagaa actggaaact ggcatcgagg ccggcgcacc cggcatagta
     2641 atgacgggcg acggaggggc gcttgtggcg gcctataccg aaatcggcga ccaggatcac
     2701 agcgtctggc tgaccaggct gcccatcaag accggggcca tcgacctgga tccggccctt
     2761 gcatcgattt cggatgaaac attcaagcac gaccagaacc ccgacctggt tttcacgacc
     2821 gcggcgaccg gacttatcga tttgcccctg acactgctgc cggatctggt tcgatccaca
     2881 agaatttcac cggttgaaac cgtcatgaca aaatgacgtc ggcccgcgat ggtggcctgc
     2941 ccgggctgga aatgccgcgt ggtttaacgt aacctgcgcc atcgacttca cggaatcgaa
     3001 gtcattccat cgacttttac gacgggtata tggagatgaa acgagcatcc ctgatacttc
     3061 caattttcat tctggcgggt atcgccggca gttacggctg cggacagacg gcgggccccg
     3121 ggctcgatgc gatccaggac gtaccggatg aagcgccgca ggatgccgtg actggcgacg
     3181 gcggcgacgc ctttaccgaa gaagtgctgc acgactctgg cgtggatggc aacgatatcg
     3241 acctggatct cggcgggaac gaattgcagc aggatcagtt cgtgttcgtg ccggtcgatg
     3301 acgcgttcgc gcccgacggg ctgcacatgg acgccaatga tgaaacggat gcctttgaca
     3361 tgcccgacgg cgtcaaggtg cgtgccgcct cgtacaacct ttatggatac cactggtcag
     3421 acgcggccca cttcggcgcc ctgataaagg aaatgaatcc cgacctgatg gcgctggtcg
     3481 aaagcaacga agaccaggtc caggccgtcg ccgaggccgc cggatatgaa tactggtgca
     3541 cgaacggtga cggccgcagc cttctgtcga taaccccgcg ggcgacttgc gagacaatca
     3601 cgcttgtcgg tgggcggacg ctgcttcgaa ccgaaaccga aatcgacggg gttacgtttg
     3661 ccgtctgggt gatgcacatc gactgggaca tcatcggtaa caggcagacc cgtgatttca
     3721 tggacaacca tttcgtcaac gagacccgca gccacgtgat aatgatgggc gacttcaatg
     3781 acgaacacct gagtcggcag atcaacattc tggaggaaca gcttgccgac gcgttcaccg
     3841 cgtacggctg gtacccgggg gaacgcgtca cctggccggc gcagtacttc gacgacaccg
     3901 aaggctcaca gacgattgat atcatcttct tcaggaagtc cttgcgcccc atcgtcctgg
     3961 acgccgacgc cgtcaatatg tcgcccgtgc tttccgacca caagcctgta tgggccgaca
     4021 tgcttttccc gcgtgacgac cagcccttcc ccgactatat gacgatgaac cggctggatc
     4081 cgtacagggt gatctggaag gttccgggga cctccgacaa cctggtcacg aaccccggcg
     4141 ccgaggatgg actggccggc tggacagtca ccggcggggg cgaggccgcg acggagcgct
     4201 tcgcctgccg ccccgccgaa ggcgaaaaat tcttctgcgg tttcccgaaa ggatacaaac
     4261 cagacgacgg gtacacacga ttcacgcagg aaatcgatct ttccgactac atcgagatga
     4321 ttgatgccaa cagggcgtgg ctgaacgttt cggcagaaat aatcacatgt cccttcctgc
     4381 tgaccgaaga cgacatcacg accaatcacg tacaacgtta cgacgacgcg gaagtcacgg
     4441 tcacgcttgt ttcggcggat ggcaaggcaa cggaccgccg catttcgaaa cgccgggaca
     4501 cgctgatgtg gtatccgtac gcggacgcct tcccgattcc atccggcacg cggaagatcg
     4561 aactggcact gaccgcgcac aaacgaatcg attacgtcgg cggcatcgat gcaggttttg
     4621 acaaggtata tgtgtcggta cgggaatggc cgatacacgg cgtgcttgat ggaaacatgc
     4681 tcatgggggg gattgacggc gattccgcaa tcgatggcgg caacgcgacg gtttccaaca
     4741 gcggatgggt caccaggacg acggggtatc cgttaagcct gaatccattt ccaccggccg
     4801 gcgtgtccgg gttgaaatac ctgttcgccg gagatgaagt cggactgaac ccgctggacg
     4861 aaccggccgt actgtcccag acgctcgacc tgtcaaagtt cggcaccgaa atcgacaagg
     4921 gcaggatgac actgcgctgg ggcggtttta ccaggacgaa taccgctgag ggtgcatccg
     4981 tcgcgatgtc gctgcagctt cttgatggac gcggggacgt atggggagaa gtccacggcc
     5041 gcaccgtctg ggagtccgaa tggacccgcc aggaaaaccg gaccaggctg ccacgcggca
     5101 tcaggggcgc ccgactggtt cttcgtgccg aagtgccgtt gtttgacgga ttccttggaa
     5161 ttgaaggcat ggaaacccag gacgatgcag tgttcgccga cgaattgttc atccgccccg
     5221 aaacgttttg atttccagga tactactggc gggcgaattt tcattcgccc gtacggggat
     5281 gccgggcaac agtctgtgct gaaccgcggc gggcggatgt aatccgcccg tacaaggagc
     5341 gggaggattc gacttggcca cattatagtc ggcgaatttc ctgccttgag catgccgaag
     5401 ggtcattcgc ccttacggag aggccggggc aacaggcggt gggttattcg ccggggccga
     5461 cgacaaccat gttttccatc ttgaacttca actggttggt catgaaccag aaccagtaga
     5521 tcccaaacgt caggccagaa aggatggttc caatcaagtt gattacgaac agcttcacgc
     5581 ccgtgccttc gaaacggaac cgattcacga cgccgttgaa ttccaccgtt gtttcctggg
     5641 cgaagtactt cgccatatcc acgataaacc aggagaagta aatgccgaac gtgataacgg
     5701 aaaggatgac gccgacaaaa accttgccga acagggttcc accagcgcca tcaaacttga
     5761 acgtgccaac ggccgagccg ttgtgcatga tgaccatgtt ccctgcgaag taccgggcca
     5821 ggttgatgca gaaccacggg cagtagatgc ccagggtgat tatggtaaga agggtgccga
     5881 caaacatcgt tccgaacagt gccccgccag tgccgttgaa ggtgccggtg tactcgcggc
     5941 catccgcgtc gcgagcgacg acgttcgccg cgaagaactt cgacacgctc acgacgaacc
     6001 acgaatagta gatgccaagc gttatgatgc tcaggataac tccgattatc catgtgccga
     6061 aaagctcggc cccgctaccg cggaaatcga acctgacctc gctgccgccg accttcatga
     6121 tcgtgttttc caggacatac ttgtacagct tcacgacaaa ccagggcaga tagattccga
     6181 gagttattat cgtaagaagc acaccgacga acagattccc gaacagcccc gcaccggtac
     6241 ccttgtaatc aaaacgcttt tccaacatgg cctccaaatg taaaatcgtg acgctttatg
     6301 ttatcagatg atttccattc ggcaacaccg gcaacaggaa ccggtcgtca acggttcgat
     6361 tagcaacggg tcgattggca aggggataca ctggtgaaga aaggccagca ggaactgtcg
     6421 ggcatacatt tcaagccttc gctgcacaag cagttgatcg gccgcctgat gggcaagccc
     6481 aagaaccttc gggaaaaggg gcttttccaa aaactggcac tgatcccatt cctggcctgg
     6541 gtcggtctgg gcgccgacgg actgtcgtca tcctcttacg gaccgatgga gtccttcctt
     6601 gcccttggcg aacactggta cctggcaatc gtgctcgccg ttgcaacagt cctgacgatt
     6661 gccgtcatca gcttcgccta tggccgcatc ataacgaagt ttccgggcgg cggcggcgga
     6721 tacgtggtcg caaacgccct gcttggcaag cacgcggccg ttcttgccgg ttcggcactt
     6781 acagtcgatt acgtcctgac ggtaaccgtg tcgatcgcgg cagccggcga cgcgttgttt
     6841 tccttcctcc cgcccgggat ggcagccgga aaactgccgt tcgaggcctt gctcattctt
     6901 gctctgatca cgcttaacct tcgggggctt cgggaagcca tcaccccctt gattccgatc
     6961 ttcctgatct tccttgttac ccatgcggcg ctgattggtg gctcgatact tcttcatctg
     7021 gccgacatcc cgaaggttga agaaggtgtt gtcgaaggct ttcggaacgg atactcgacg
     7081 ctcgggatca tgggaatgct cgccatcttc gcgcgggcct ggtcactcgg cggcggttcc
     7141 tataccggaa tcgaggcagt gtccaacgga cttccgctgc ttcgcgaacc acgcgtcagg
     7201 acggcgcacc gcacgatgct gtacatggcg gtttcacttg cattgacggc tggcgggctg
     7261 ctgattgggt acctgcttct tggcgtgcgc ttcgaagagg gcaagaccct caacgccgtc
     7321 ctgtctgaag ccgttttcgg cacggggaca ttcggcagag ccctggttat cgccacactg
     7381 gtcagcgaag gcgcgatgct tgtcgttggc gcacaggccg gctttatcgg cggcccaagg
     7441 atccttgcca acatggccat ggatggatgg gttccacgcc gctttggcgc tctttcatcg
     7501 cgactgactt cgggccacgg catcctcgtg atgggagtcg ccgcgcttgc ggctcttgcc
     7561 tatacgggcg gcaacgtttc gacgcttgtc gtcatgtacg ccatcaacgt ctttcttaca
     7621 ttttcattgt cgatggccgg aatgctgaag ggggctctgt tcccgggcaa gggtgacaaa
     7681 cgaaacttcg gcgatgcggc cctgttcctg accgggctcc ttatttgcct gtcaatcctg
     7741 ataatcacca cctgggaaaa attctatcag gccggctggc tgacccttgg ggtgaccggt
     7801 gcaatcaccg tcgcgtgtta cctggtcaag cgtcactatc gcagtatcgg ccgacttatc
     7861 gccagcctgg atcgccagat tccacgcctt ccagagattc cggaggaacc gccgcacgac
     7921 tccggccaca acgggcgaac ggcagccatt cttgtcggcg ggtacggcgg ccttgggata
     7981 aacaccaccc gggaggtcgt gcgggagttc cgggacgtat tcaagcgatt tgttttcgtt
     8041 tcggttggcg tggtggacgc ttcgtcgctg caatacgggg tcgaggcgga acaggttctc
     8101 gaccgcacga aggcggcgtg ccgccagtat gtcgaacttg cgtcgcgtta caaaataccg
     8161 gcttcatacc gggccgcggt cgccaccagc gtcaacgatg aactggaagt gatatgccag
     8221 cgcgtggcgg cggattacga cgacgtgacc ttcttcgccg gccagctcgt atttgaccgc
     8281 ccgaggtggt accaccagtg gcttcataac aacaccgcgt tcgacctgca gaaccgactc
     8341 ctggtgttcg gccagacact tgtcatcctg cccaggcttg tccagcttgg cggcgttgac
     8401 accgggaagc tgccggacaa atgaatgtgt tcgggcgaat ttcctgccct gagcctgccc
     8461 tgagcgagcg cagcaagtcg aagggtcatt cacacctgct gaacggctgg gtgctgggca
     8521 gcgccggctg ttacattttt cactgcaata atcgtaactt ttgtaaatca gattaacttt
     8581 gtccgatttt tctgataaac accatttcgt ggctttgaaa attcccctgg aggaaaaaaa
     8641 tggacatgtt ctgttaccag tgcgagcaga ccgcgaaggg tattggatgc acggttgccg
     8701 gcgtgtgcgg caaggacgct gtgacatcgg cgatgcagga cctgctgctt taccagtgca
     8761 aggaaatcag cttctacggc agcaaggccc gccagatgaa gctgtcatcg cccgccatag
     8821 accgcttcgt cgtcgaaggg ctcttcacga ccgtgacgaa cgtgaatttc gacgccgaca
     8881 agatcgtcga aatcgttcac cgcggcgcgg accttcttga tctggcgatc gaaatggtca
     8941 atgacgccgc gatggcgaac ggggtcgaac ccgaagcaat caacaggccg gttccgaact
     9001 tccgcggaag caaggaagaa ctgctggccc tggcgctgaa gaccggcatc cgtgaaaggt
     9061 ccgagcagcg cggtcccgac gcgacaggcc tggaagaact gattacgtac ggactgaagg
     9121 gcatgtgcgc atatcttgat cacgcgatgg tgcttggccg tttcagcaac aacgcggtcg
     9181 gcttcctgat ggatgcgctg gccagcctgc tgaatccaca gtcggtcggg gatcttaccg
     9241 ccctggcgct gaaggtcggg gaagtcaacc tcgaggtcat gggtctgctt tccgaagcga
     9301 acaccggcac ttacggccat ccggttccga cgaaggttcg catcgagccg gtcaagggca
     9361 aggccatcct tgtttcgggc cacgacctgc tggatctgga agccctgctg aagcagacgg
     9421 aaggcaaggg aatcaacatt tacacccacg gcgagatgct tccgtgccac gggtacccgg
     9481 gattgaagaa gtacgcgcac ctggtcggaa attacggcgg cgcatggcag gatcagcgca
     9541 aggagttcga tgaattcccg ggcgccatcc tgatgaccac caactgcatc cagaagcccc
     9601 gcgagacata tgccaacagg atcttcacga ccggcctggt cgagttcccc ggcctggttc
     9661 acgtcacccg cgaggacttc ggcccggtca tcgaggccgc gcttgaggcc cctggttttg
     9721 aagcggacgg tcccgatcgc acaatccttg tcggtttcgg ccacaatgcc gttcttggtg
     9781 tcgccgacaa ggttatcgaa ggcgtcaagt cgggcgcgat caagcacttc ttccttatcg
     9841 gcggctgcga cggcgcgaag ccgggacgca actattacac cgactttgcc gaaaaggccc
     9901 cgaaggacac ggtaatcctg accctggctt gcggaaagtt ccgcttcaac aagctggaat
     9961 tcggcgatat cggcgggatc ccgcgtttgc ttgacgtcgg acagtgcaac gacgcctact
    10021 cggcaatcca gattgctgtc gccctggcca acgctttcgg aacggacgtc aacagcctgc
    10081 cgctgtcgtt catcctgtcg tggtatgaac agaaggccgt ctgcatcctg ctgaccctgc
    10141 tgcacctggg aatcaggaac atcaaactgg gaccgtcact gccggctttc gtgaccccgg
    10201 cggtcctgga cgtcctggtc aagacgttca acatcgggcc ggtcaccacc gtggaagccg
    10261 acctggcggc ggccctgggc aactgaccct cacctgcccg gctgatcaat cccgccatct
    10321 tcagccagac gttccaatgt ttgttcgcca tcacccgaca atgttgtgat aaataatcac
    10381 ccatgacgga gttctgacca tggacagaaa catcacaccg gataacttcg aggcgtggaa
    10441 tgatgagatg ggccgaaaat tcgaccccga tgcctatcac aaccacccta atccgctgat
    10501 acggaacatt gaaaggaagc gggtccggac ggtgctgaag ttcatcaagc cgaccgaggg
    10561 ccagcgtgtc ctggaagtcg gctgcggggc cggcaacgtg ctggaacaaa ttgtcggcgc
    10621 cgaccttact ggaatcgacc tttcagacca tctgcttgaa aaggcccgtg agaggttgcg
    10681 ccagcgtgcc cgcctgctga aggccgacgc cgcggccctg cccttcgaag acggatggtt
    10741 cggcgtcgtc tattgcaccg aggttctgga acacgtcctg gatccgcgtg ccgtcatcag
    10801 ggaaatgcgc cgcgtgcttc gcccggatgg ggttgccgtc gtgtccatcc cgaacgaatc
    10861 cgtgattaac ggaatcaagg gtcactttct tgacaacccg attggcagag tcctgctgaa
    10921 tgaaaagccg ggaacatacc attcctcgaa aaagatggac gatgaatggc accttcattc
    10981 cttcgacttg aagatgctga aatgcatctg cgagggcgta ttccggattg accgccttgc
    11041 cgcgattccg tcacccgtcc ttcccataag gtatgttgcc aggctcgcgc cgatcaagcc
    11101 cgcatctcat gcgaaaacgg ctgaatctga aggttgaccc ccggtattgg cggatctaga
    11161 ctccgaccag cgtaacgggg gaatgccatg accgctttca tggtggcgct ggccttgctg
    11221 gccgcgggat accttcttta cggacgactt gtcgaacgcg cggtcaagcc gaatccggac
    11281 agtccggtac ctgcaaatag ttgtgccgac ggcatcgact tcgtagccat gccgacgtgg
    11341 cgcgttttcc tgatccagtt gttgaacatc gccggtctgg gccctgtttt cggggccatc
    11401 atgggcgcac tctgggggcc ccaggtattc ctgtgggtcg tcttcggatc gatccttggc
    11461 ggcgccgtcc acgacatgat gtcgggcgtg atgtcggtcc gtaacaatgg ggcaagcctg
    11521 ccggagctta tcggccggta ccttggcaaa gttccaagac acatcaccac gctgttcatc
    11581 ctgatcctga tgatccttgt cggcgcggtc ttcgtcaaag gcccagccga actgattgtc
    11641 cagttgattc cggcccagcg catcggcgat ctgatcggcg gaaacaccgg ggaactgctg
    11701 gcggccgact ggcgcggaag cagcgtctgg ctgtggtgcg taatggcggt catcttcgcg
    11761 tactacattc tcgccacgct tcttcccgtc gataaaatca tcggccgcat atacccgata
    11821 ttcggcgcca tactgctgat catggttctg ggcctgcttg tcgccatggt caccggacgt
    11881 atcgacgtgg tgccgttcac gctcgaaaac ctgcatccaa agcagacccc ggcatggcca
    11941 accatcttca tcaccgtcag ttgcggcgcg gtgtctggct ttcacgccac gcaaagcccc
    12001 ctgatggcgc ggtgcctgaa atcggaaaca aggatgcgag ccgtcttcta cggttcgatg
    12061 attgtcgaag cggtcatagc gttgatctgg gccgcggcag ccgccggcta ttatggcggt
    12121 tcggccggtc ttgccgccgc catcggcccc ggcaacaacc cggcgccgat agtcatgcag
    12181 gtctgcaagg gaacgatggg tacgatcggt ggcatcctgg ccgttctggg cgtcgttgtc
    12241 ctgccgatca cctcgggcga cacggctttc agggctgcca ggctcatcgc tgccgactac
    12301 ctgcgcctgc cccagaaaaa gatcctgaac cgttacatca tcgcaatacc gatgttcgtg
    12361 atatcggcaa tgctgaactt cgttccgttt ggggtcgtct ggcgctattt tgggtgggcc
    12421 aaccagacgc tggcagccgt cacgctgtgg gccgccgcgg catatctggt acgacgcggc
    12481 ggattctggt ggcttatggc cgccatcccg gcgacattca tgaccgtgat gacggtaacc
    12541 ttcttcgtcg tgtcaaaaaa cctgagccac atcgggatgg atattgttcc cggaaccatt
    12601 gtgggactgg gcttcggctt gacgtcgatt gtcgcattca tatggctgat gaaaccgatg
    12661 gggcggagtt gtcacaagca ttcatcgcct tgatcgacca cttcccagga agaatcgcag
    12721 atcttcaatg agctgaaccg ggtcgcttgc aacgattgtc gggttcattc tcagcgacgc
    12781 ctcgattgca ccggaggaaa cacaatccgc ttcgcacctt cgtacccgtg atcgcagcct
    12841 ttccatcccg ggcccgcgcc aggcagcctt caatccgact tcacgcaccc cggacgcctt
    12901 gtccatgatg tcgtagcacg gaaagaggtt tccgtaagga tctatattgc cgaacaggta
    12961 tccggcccag cactgaaagg ccctgcgtga cttcgggcca ccggcggcca tgctgttgaa
    13021 gcggcgcgag ttcgtgtgca ggccgcgtga ccgggccagg gccgtcgccg ccagcatggc
    13081 gcgatgaacg gtttccgggt ctgccggcac caggggcacg catgaatcat ccccgaagcg
    13141 ccactggtgc tgaagattgg catgcaaagg aagaaagctg atgccaccga cgccgaggcc
    13201 gtccgccagt tcaaccatcc gttccaggca atcgacgttg tggcttccga cgacacaatt
    13261 gattccgaca cttatcgatg gacgcagttc ccgcagaagc cgcgcggccc gcgtgactcc
    13321 ggcgtggctt cccggcccgc gcacgcggtc gttgacgtcg gcgtcggggt gatccaggct
    13381 gaaaatgatt gtgacatcgg ccccatccgg gatcgccccc acgacttcct cgtcgatcat
    13441 ggttccgttg ctgtcgatat ggacattcag cccaagcgac cgcgcaaacg cgacaatttc
    13501 aatgaaatcg gggcgcatga acggctcgcc accgccgaat gacacgatcc tggtcttcaa
    13561 atcaacacaa tcgcgcagaa tcgccctgac ctcttccgtc gaaagctcgc ggccatagtc
    13621 cggatagaat cgcggatatc cgcagtactg gcaggaaagg ttgcaccgcc tggtgatttc
    13681 gaacatcagc aggggcattg tcatcggaga acgtcgcgca agcctggcaa ggtgcagatc
    13741 gatgaactcg attccaagca ggccgacctg agcggccatt ccggcgattt ggctgatgcg
    13801 gctcaaaatc tgctcgcccc ccgaagcccc gccccgatta gacatgccca ggacgggact
    13861 gtcaacagag ggcccccggc gcgcattccc cggatactga gccgcggtgc ccctggtgat
    13921 tgtgtcaaga cagtcgatgt cgcataatcg acgacggaat tcaccacagg aatcgcgatg
    13981 agaaagactt cattcgcttt ccggataatc tgtccggcat gtgccattcg cctggctcct
    14041 gccgcgact
//
LOCUS       DAOPPA010000016        13691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5241_length_13691_cov_3.617190, whole genome shotgun sequence.
ACCESSION   DAOPPA010000016 DAOPPA010000000
VERSION     DAOPPA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5241_length_13691_cov_3.617190"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PKH54_01075"
     CDS             <1..1587
                     /locus_tag="PKH54_01075"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOC98505.1"
                     /translation="VPDMTDDVIGDDSGSDAVADIPVADAPGDVMPDVDLDAVDAADV
                     EPDVPVVPDPALRLREQGWLIGDLHMHSTYSDGEDSVAVTVGLAEYFTDPDFLAFNPE
                     YDGFALDYIALTDHRTAIQNSDPDFFSEKVVLIPGEEFGGPGHACLFGVTDTVNHNAN
                     GGPTTTEDYFDGLEVAGAMGAVRSINHPFTTGIYFPWDLHNYESMEIWNVKWAVSQPG
                     ASQATIDQWEAEKGATASVQFRKAAEYQGIGGNAQALKFYEVLLSRGHHIAVVGGSDR
                     HTVFGIGFPATWIKSTSRDVSGILDAIRARHTFVNRTPIAATIEMTVEAGDETFMQGD
                     QIPIPAEGLEVTIKLRTTRAAGGRLFLIGGHAIASDLDLEGAVISENDLMVAIGTDDF
                     QVEYTTTVMPGDWFYPVVHEPLVQPGLDPDLTAQIPEIAATVAQISGENYSPLIGALF
                     PYVDDLTFMSPENCDPAGWVAGKAQCLPPDNNGMATIMLPDWIDRVLNVVVENGQSST
                     EWCMGAAASAVLFVEKGQPE"
     gene            complement(1597..3078)
                     /locus_tag="PKH54_01080"
     CDS             complement(1597..3078)
                     /locus_tag="PKH54_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98506.1"
                     /translation="MRTEIALMVALLSITAGLQVGCGGECGCADIIGIDINTPDEGVD
                     TIPTDDGADTGRDSVEPLDQGLDNGFCDILHDHIPTDQVPVDSVNEVTSDIGPAHECD
                     TEYDCDGCDICTELQGVRKCLATPEAGMKECYDLVPCEEGNSCHYDNVFRPECGGYCA
                     ANAEYRLAEWGVNVVTPEGGATMSAAPKQYYGAVAAKPVIYIYSEQQFTLDVGVRYDE
                     GIAYETWPEIPLSANVVWENVQVGTTECTPTATPVPDPFGMIEPPSLEIYELPEWVIP
                     EANCLTHGETISKLLFYTGPFVGYQPPVAASLSIDQGAGMATLSITNGLDAAIPKVMV
                     IYRDTLSNCMDPSYCPVHTARIAWGITGQVNAGETMNQDFTYAEYTVEATEQEPYPTV
                     EGLLPEGWLEMPANLKAELQTAGLTEDEAGVFMNAWTQSMFGLLGQDADWYFPSYENG
                     AFVIYIWPESRVEQKLPMAAIPAPTTKNRVIVEYQRIAHEFAQ"
     gene            complement(3189..4118)
                     /locus_tag="PKH54_01085"
     CDS             complement(3189..4118)
                     /locus_tag="PKH54_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC98507.1"
                     /translation="MQDKAGKTVSTGFGNQAASPVIDPGKCTNCGMCVKICPSVTIIN
                     VSGQATVVPDNGMGCFACGQCTAICAFDAVRVTGRRMTPDDVQPWPASDRTADDVSLH
                     NLMLTRRSVRTFKDAPVERELIDRILEMTASAPMGIAPSDVECTVFLGRDRVQAFAGD
                     LDGVFRKWNSFFNPVTMFFMRVFGSKAAYQSFDGFLLPIIREMIHAREHGSDMLFYDA
                     PCVLLFTTHPMADPIDGTIACTYAMLAAQSLGLGTCMIGTVAHAVNMDKKVKARWGIP
                     ADHKACLAMVVGHPAHRYHRVVKRTLAAIHIKD"
     gene            4241..5005
                     /locus_tag="PKH54_01090"
     CDS             4241..5005
                     /locus_tag="PKH54_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC98508.1"
                     /translation="MEKLGTSVPVSGRRCKVVCIKRNTADSVEILLKDESGEPFVFKP
                     GQFFMVNVEIDGQVVRRTYSATNAPGEFDGALRLGVKRRPGGLVSGYMNDRLKVGDEV
                     VVKGPGGKLVPTEGRGPRHLVLIAGGSGVTPMISMARTLLASEPDTSISLIYCNRCLD
                     DVMFLQELNELVDSNHDRFRVFHVLKENAEGWSACAGRLNVDILRDILNGLPAVDEYL
                     VCGTPEMMTGVCGYLAELGVPASVIRTETFNVPEDI"
     gene            5124..5762
                     /locus_tag="PKH54_01095"
     CDS             5124..5762
                     /locus_tag="PKH54_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOC98509.1"
                     /translation="MLQKILPKNYGFFDLFENHAAAVLKAALVMDELLKELGTPNAIS
                     HVRRIEELEHECDNYTHMTVDLLRRTFITPLDREETSTLISALDDIADLIQAAAHRVE
                     FYDINSCPDGVRQLNGVLMKAVEQLVQAVGFLREMKKHAAQIPVICMEINRLENEGDR
                     VKRTGVGELFRSRMDPLELMKIKELYESLENAIDKCEDAANIVEGLLIQHVG"
     gene            5871..6917
                     /locus_tag="PKH54_01100"
     CDS             5871..6917
                     /locus_tag="PKH54_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC98510.1"
                     /translation="MQFLEALTNVEPLIVITLVAALAFDFVNGFHDAANSIATVVATR
                     VLSPKFAVAWAAFFNFVAAFTFGTAVAKMVGKDVVDLSFVTVPVVFAGLMGAIIWNLI
                     TWWLGLPSSSSHALVGGYAGAALAKSGIAALKFAGLAKIAAFIVIAPMVGMMLGMLNQ
                     IIVMWIVRRQPPGKLFPIFRKLQLLSAAIYSFSHGSNDAQKTMGIIFALLVASGLPPA
                     MWYQDPTNPEHIPVWIILICHAFIAFGTLFGGFRIVKTMGSKLTKLDPMHGFCAETGG
                     GVTILAVSALGIPVSTTHTITGAIIGVGTVSGLRSVRWIMAKKIVWAWIFTVPMAGLF
                     GWLTFRLMAMLGQG"
     gene            7022..8068
                     /locus_tag="PKH54_01105"
     CDS             7022..8068
                     /locus_tag="PKH54_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC98511.1"
                     /translation="MDFASIFQHVDPLVIITLIAAIAFDYVNGFHDAANSIATVVATR
                     VLSPRFAVAWAAFFNFAALFVVGTAVAKMVGKDVVDLSYVTTAVIFSGLTGAIVWNLI
                     TWWFGLPSSSSHALVGGYAGAALARSGIEALKLAGIGKIAAFIVIAPIIGMILGMANQ
                     IIVTWIVRKMPPGKLYPLFRKLQLLSSAVYSFSHGSNDAQKTMGIIFALLLASGIPGN
                     VWGHDPVHPDSIPLWIEVLCYAAIAAGTLSGGLRIVKTMGTRLTKLDPMHGFSAETGG
                     GVTILGVSFLGIPVSTTHTITGAIIGVGMVGGLRSVRWILARKILWAWLFTIPASGLF
                     GWGIYRLMSLFGHN"
     gene            8188..8775
                     /locus_tag="PKH54_01110"
     CDS             8188..8775
                     /locus_tag="PKH54_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOC98512.1"
                     /translation="MTVNNDKGLIGKVLSAVVVFGAAVAGIGSAGCATTTERLNLQPP
                     TTVAKVDLNRYLGKWYDIASFPQWFQKGCTATTAEYSLKDNGEIKVVNSCRKGSLDGK
                     QKVSVGRARVVDKDTNSKLEVSFFGPFWGNYWILDLGENYEYSVVGDPSRDYLWILSR
                     TPQMDGEVYRGIVERLKAQGYELHRLQKTLQPGDM"
     gene            8795..9256
                     /locus_tag="PKH54_01115"
     CDS             8795..9256
                     /locus_tag="PKH54_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOC98513.1"
                     /translation="MRLWSIHPRFLDTRAIVAVWREGLLARAVLSGKTRGYLNHPQLR
                     RFRDQPNPISALDAYLSEIVVEAESRGYDFDRSKIVFGRAPSPIMDVTAGQLLCEWIH
                     LNRKLAVRDPVRLVLQFARNPAASPFFRLVAGPRADWEKAADCGMSGSATA"
     gene            complement(9180..9599)
                     /locus_tag="PKH54_01120"
     CDS             complement(9180..9599)
                     /locus_tag="PKH54_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOC98514.1"
                     /translation="MKLKKALLIIAGVACLVLAGIGVLLPLLPTTPFLLLAAAAFLRS
                     SDRLYRWMHENRVFGEYLRRYRDGLGIPLASKIVSISLIWITLITSVVFVMPHRLLWV
                     GAVLFAIGTGVTVKLLRIPTYRNQPPSPSRPSGRPPA"
     gene            9775..10152
                     /locus_tag="PKH54_01125"
     CDS             9775..10152
                     /locus_tag="PKH54_01125"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC98515.1"
                     /translation="MKDVAAFFKVLSDPARVQILWLLFNNSELCVCDIMGTLRITQSK
                     ASRHLATLRHAGLVTDRRDGAWSYYSLKQPRDVLEAEVIGILKSRLALLPDAGSLLVA
                     LGHWLDVKAGRQSCCSLSGGGDE"
     gene            10145..11035
                     /gene="arsM"
                     /locus_tag="PKH54_01130"
     CDS             10145..11035
                     /gene="arsM"
                     /locus_tag="PKH54_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOC98516.1"
                     /translation="MSKADDVRKMVSESYSAAVKAALARRKAKQAKSAPSKSCCCGGA
                     PKISEQSLAELAGYSREALAGLPPDAVTSSFGCGNPVALAAIREGDTVLDLGSGAGID
                     LLLAAGLAGPSGRVIGVDMTPAMIERARANAEKAGLKNVEVRQGIIEDLPVESGTVDL
                     VISNCVINLSPEKNRVFAELARVLKPGGRISISDICAEMMPDWAIQAGHLYSACISGA
                     IPEAAYIQGLVEAGLEDVKVTDRLVYDAQQLSGLLGSVESRGCCGGSSALTPAQLERA
                     VAELVGTIASLRFVGMKPVV"
     gene            11051..11464
                     /locus_tag="PKH54_01135"
     CDS             11051..11464
                     /locus_tag="PKH54_01135"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOC98517.1"
                     /translation="MKGMLFLCVANSARSQIAEGLARSMVPPGVNVLSAGSSPTFVRP
                     EAIAVLKEIGIDISGQRSKSVTEIPPDLVDTVITLCGEQECPLFLGRATRLHWGLEDP
                     AAVQGSEEGRLNAFRRTRDELISRLAEFIGQGGVK"
     gene            11470..12657
                     /locus_tag="PKH54_01140"
     CDS             11470..12657
                     /locus_tag="PKH54_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC98518.1"
                     /translation="MKERTKLILIVAAFAALWFLPVENVVFMGAIRESLALAKWYARE
                     HVILCLIPAFFIAGAISVFVSQGAVMKYLGPKANKALAYGVAAVSGTILAVCSCTVLP
                     LFSSIHKRGAGLGPAIAFLYSGPAINVLAIIMTARILGPELGIARAVGAVVFAVVIGF
                     LMHLIFRKEEAERQSSGVVIIDDQEKTRPLWKDGLYFAAMVGILVFANWGKPADGDGG
                     VWAAVHSVKWIVTGVFLVGLIASIVAWFRKDELATWVDSSWTFAKQILPLLFGGVLVA
                     GFLLGGPGGSDKGLIPNAWIQGLVGGNSLLANLFASMVGAFMYFATLTEVPILEGLIK
                     SGMGQGPALALLLAGPSLSLPSMLVIRSVLGTKKTLVYVGLVVVLSTVAGWTYGLIVH
                     AAA"
     gene            12693..12941
                     /locus_tag="PKH54_01145"
     CDS             12693..12941
                     /locus_tag="PKH54_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC98519.1"
                     /translation="MTVKVLGTGCPKCKKLYAEAEKAIAESGLSVGIEKVEKINEIMD
                     YGVMMTPALVVGDQVKSTGRIPATAEIVAWIVEAAKTA"
     gene            12967..13482
                     /locus_tag="PKH54_01150"
     CDS             12967..13482
                     /locus_tag="PKH54_01150"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOC98520.1"
                     /translation="MQNPTCRFFVPTLLSLILVSAASCGSSGPTDRNAAVAASSGQVS
                     TVPASAPAPVLQRERIVAYYFHGDRRCRTCLGIQETIERTIAERFPAETASGRLEYRE
                     VNFEQEQNKHFVQEFQLSFGTMIVAKVDDDKILEWENCGKVWDFGHEPPLLVDYVAER
                     INAYLSKLKAE"
     gene            13482..>13691
                     /locus_tag="PKH54_01155"
     CDS             13482..>13691
                     /locus_tag="PKH54_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC98521.1"
                     /translation="MDSMLFAVGSALWLGIMTSISPCPLATNIAAISYVGKRVDRPGL
                     VMLSGLLYMLGRMLSYFVVALVVVKS"
BASE COUNT         2651 a         3779 c         4179 g         3082 t
ORIGIN      
        1 gttccggaca tgaccgatga cgtaatcggg gacgattctg gaagtgacgc cgttgccgat
       61 attcctgtgg ccgatgcccc cggcgatgtc atgcctgacg tcgatctcga tgcggttgac
      121 gcggcggatg ttgagcccga tgtccccgtt gttcccgatc cggccctgcg tctgcgcgaa
      181 caggggtggc tgattgggga tctgcacatg cactcgactt attccgacgg cgaggattct
      241 gtcgcggtca cggtcggact tgccgagtac ttcacggatc ccgatttcct ggcgttcaat
      301 ccggagtatg acggattcgc actggactac atcgccctga ccgaccaccg gacggcgatc
      361 cagaacagcg acccggactt cttcagcgaa aaggtcgtcc tgatcccggg cgaggagttc
      421 ggaggtcccg gtcacgcatg tctgttcggg gttaccgata cagtcaatca caatgcgaac
      481 ggcggcccaa cgacaaccga ggattatttt gatggccttg aagtcgcggg tgccatgggg
      541 gcggtccggt cgattaacca tccgttcacg accgggattt actttccctg ggatctgcac
      601 aattacgagt cgatggaaat ctggaacgtg aagtgggccg tcagtcagcc gggagccagc
      661 caggccacca ttgaccagtg ggaagccgag aagggcgcga ccgcgtcggt gcagttcagg
      721 aaggccgccg agtatcaggg aatcggtggg aacgcccagg ccttgaagtt ttacgaggtt
      781 ctgctgtcac gcgggcacca catcgccgtc gtcgggggaa gcgatcgtca tacggtcttc
      841 ggtattggtt tcccggcaac ctggatcaaa tcgacctcgc gtgatgtctc tggaatcctt
      901 gatgcaatca gggcccgtca tacgttcgtg aaccgcactc cgatagcggc gaccatcgag
      961 atgacggtcg aagccggtga tgaaaccttt atgcagggcg atcagattcc gattccggcc
     1021 gaaggccttg aagtgacgat caagctgcgc acgaccaggg cggccggtgg ccggctgttc
     1081 ctgatcggtg gacatgcgat cgcttcggat ctggatctgg aaggtgccgt gataagtgaa
     1141 aacgacctta tggtcgccat cggaaccgac gacttccagg tcgaatacac gacaacagtt
     1201 atgccgggtg attggttcta tccggttgtc cacgagccgc ttgttcagcc gggactggat
     1261 cccgatctga ccgcgcagat tcctgaaatc gcggcgacgg tcgcccagat ctccggcgaa
     1321 aattattccc ccttgatagg ggctcttttt ccgtacgttg atgatttgac gttcatgagt
     1381 cctgaaaact gcgacccggc aggatgggtt gccggcaagg cacagtgcct gccgcccgat
     1441 aacaacggga tggcgaccat catgctcccg gactggatcg acagggtctt gaacgttgtg
     1501 gtggaaaatg gccagtcatc gacagaatgg tgcatgggcg ccgccgcaag cgcggttctt
     1561 ttcgtcgaaa aaggccagcc ggaatgaatc tggtcgttac tgcgcgaatt cgtgcgcgat
     1621 tctctggtat tcaacgataa cgcggttttt cgtggtcggg gctggaatgg ccgccatcgg
     1681 cagcttctgc tcaacgcgtg attccggcca gatgtagatg acaaaagcgc cgttttcata
     1741 cgacgggaag taccagtccg catcctgccc gagaagtccg aacatcgact gggtccacgc
     1801 gttcatgaaa acgccggctt catcttcagt caagccggcc gtctgaagtt cggccttcag
     1861 gtttgccggc atctcgagcc agccttcagg cagaagtccc tcgaccgttg gataaggttc
     1921 ctgttcggtc gcttcgacag tgtattcggc gtatgtgaag tcctgattca tcgtttcccc
     1981 ggcgttgacc tgaccggtga ttccccaggc aatccttgcg gtatggaccg ggcagtagct
     2041 tggatccatg cagtttgaaa gcgtgtcgcg gtaaatcacc atcacctttg gaatcgcggc
     2101 gtcaaggccg ttggtgatgg acagcgtcgc catgccggcg ccctggtcga tggaaaggct
     2161 tgcggcgacc ggcggttgat accccacgaa cggccctgtg taaaacagca gtttcgaaat
     2221 cgtttcgccg tgcgtcaggc agttcgcttc cgggatgacc cattcgggca gttcgtatat
     2281 ttccagtgac ggcggctcaa tcattccaaa tggatccgga accggcgttg cggtcggcgt
     2341 gcattccgtc gtgccaacct ggacgttttc ccagacaacg ttggccgaaa gggggatttc
     2401 cggccacgtc tcgtaagcga tgccttcatc gtaacggacg ccgacgtcaa gcgtgaactg
     2461 ctgctccgag tagatgtaga tgaccggctt ggccgccacc gcgccgtaat actgttttgg
     2521 cgccgccgac atcgtcgcgc cgccttccgg ggtcacgacg ttgacgcccc attcggccag
     2581 gcgatactcc gcgttcgccg cgcagtatcc gccacattca ggtctgaaga cgttgtcgta
     2641 gtggcaggaa ttgccttcct cgcacggcac caggtcgtaa cattccttca tgccggcctc
     2701 cggcgtggcc aggcacttgc gcacgccctg caactcggtg cagatgtcgc agccgtcgca
     2761 gtcgtattcg gtgtcgcatt cgtgggcagg cccgatgtcg gacgtcactt cgttcaccga
     2821 gtccaccggc acctgatcgg ttggaatatg atcatgcaga atgtcgcaga aaccgttgtc
     2881 cagcccctgg tcaagcggtt caaccgagtc ccgcccggtg tcggccccgt cgtcggtggg
     2941 aatcgtgtcc acgccttcgt ccggtgtgtt gatatcaata ccgatgatgt cggcgcagcc
     3001 acactcgccg ccgcaaccga cctgcaggcc ggctgtaatc gacagcaaag cgaccataag
     3061 ggcaatttct gttcgcatga ctaaatcccc tgcggaagtt tggaaagatg tccgtttaac
     3121 ttcgtcagtt tacgacttgg gcatgttgga atcaattcca ggtcgggcaa cgggccttgc
     3181 cgcccggctc aatccttgat gtgaatcgcg gccagcgtac gtttgacgac gcggtggtag
     3241 cggtgtgccg ggtgaccgac gaccatcgcc aggcatgcct tgtgatccgc gggtatgccc
     3301 catctggcct taaccttctt gtccatgttg accgcgtgcg ccacggttcc gatcatgcag
     3361 gttccaagtc ccagggactg cgctgcaagc atggcgtaag tacatgcaat tgttccgtcg
     3421 atcgggtcgg ccatcgggtg cgtcgtaaac agcagcacgc agggagcatc gtaaaagagc
     3481 atgtcgctgc cgtgttccct ggcgtgaatc atttccctga taatcggcag aaggaatccg
     3541 tcaaacgact ggtaggcggc cttcgacccg aacacccgca tgaagaacat ggtgacaggg
     3601 ttgaagaaac tgttccattt ccggaagact ccatccaggt cgccggcgaa tgcctgaacc
     3661 cgatcgcgtc caaggaaaac ggtgcattcc acgtccgatg gcgcgattcc catcggggct
     3721 gacgccgtca tctcaagaat gcgatcaata agttcccgtt caaccggggc atccttgaag
     3781 gttcttaccg aacgccgcgt cagcatcagg ttgtgaaggc tcacgtcgtc ggccgtcctg
     3841 tcgctggccg gccacggctg cacgtcgtcc ggcgtcatgc gtctgccggt gacgcgaacg
     3901 gcgtcaaagg cgcagatggc cgtgcactga ccgcaggcga agcaccccat cccgttgtcg
     3961 ggcacaacgg tggcctgccc ggaaacattg atgattgtta ccgacgggca tatcttcacg
     4021 cacattccgc agttcgtgca cttgcctggg tcaatgaccg gcgatgccgc ctggttgccg
     4081 aatcctgtcg aaacggtctt tcccgctttg tcctgcatat gggcttcccc gaaatgaatc
     4141 agcgattgta tatcagcaag aaacggaaat tgcaccaacg gcagccatcg tgattgcttc
     4201 cgcgccgtaa ggcgatagaa tgcacgcagg gaggcaaatc atggaaaaac tcggtacttc
     4261 cgttccggtg tccgggcggc gatgcaaggt cgtctgcatc aaaaggaaca ctgcggattc
     4321 ggttgaaatc cttttgaagg acgagtccgg tgaaccgttc gttttcaagc cgggtcagtt
     4381 cttcatggtg aatgtcgaaa tcgatggtca ggtggttcga cgcacttatt ccgccaccaa
     4441 cgctccgggc gagtttgacg gcgcgctgcg gcttggcgtg aagcggcgtc ctggcgggct
     4501 ggtcagcggg tacatgaatg atcgcctgaa ggtcggtgat gaagtcgtgg tgaagggccc
     4561 cggtggcaag ctcgtgccga cggaagggcg cgggccaagg catctggttt tgattgccgg
     4621 cggaagtggt gtgacaccga tgatatcgat ggcacgcacc cttctcgctt ccgagccgga
     4681 cacttcgatt tcgttgattt attgcaacag gtgtctggac gacgtcatgt ttctgcagga
     4741 attgaacgaa ctggtcgatt cgaatcacga tcgtttcagg gtcttccatg tcctgaagga
     4801 aaacgcggaa gggtggtccg catgtgcggg acggcttaac gtggacatcc tgcgggacat
     4861 ccttaacggg cttccggcgg tcgatgaata tcttgtctgc gggactccgg aaatgatgac
     4921 cggtgtgtgt ggataccttg cggaacttgg ggttcccgcg tcagtgattc gcacggaaac
     4981 ctttaatgtt ccagaggata tctgacttat agacagatga tgggattgct aacttattcc
     5041 taacttgaag gcttccggga tcgggtgata cattccgccc ggcataaccg gaatacctgg
     5101 gcctgaacgc caaggagaat tgaatgcttc agaagatcct tccaaaaaac tatggattct
     5161 ttgacctttt tgaaaatcat gccgccgcgg ttctgaaggc ggccctcgtg atggacgaat
     5221 tgctgaagga actcgggacg ccgaacgcga tttctcatgt caggcggatc gaggaactcg
     5281 agcacgagtg cgataactac acgcacatga ccgttgacct gcttcgcagg actttcatta
     5341 ccccgcttga ccgcgaggaa accagcacgt tgataagcgc ccttgacgac atcgccgact
     5401 tgattcaggc ggcggcccat agggttgaat tttacgacat caattcatgc ccggacggtg
     5461 tcaggcagct taacggcgtt ctgatgaagg ccgtggaaca actggttcag gccgtcggat
     5521 tcctgcgcga gatgaagaag cacgcggccc agattccggt catctgcatg gaaatcaacc
     5581 gccttgaaaa cgaaggtgat cgtgtaaagc gcacgggggt cggcgaactt ttccgcagcc
     5641 ggatggatcc gctggaactg atgaagatca aggaacttta cgaatccctg gaaaatgcga
     5701 tcgacaagtg cgaggacgcg gccaacatcg ttgaaggcct gctgatccag cacgtgggct
     5761 gatacggcaa atatccctgt cccgtggcgt taatttgagc gtgataacgt gaacgggctg
     5821 gtgtgtccgg ttttccgcgg tcgtctggat gaccgcggcg gaggttgcgg atgcagtttc
     5881 tggaagcgtt gaccaacgtt gaaccattga ttgtgataac gcttgtcgcg gcgttggcgt
     5941 tcgatttcgt caacggattt cacgatgcgg ccaactcgat tgcaaccgtt gtagcaaccc
     6001 gtgtcctgag tcctaagttt gcggtcgcat gggctgcatt ctttaatttc gtcgcggcgt
     6061 tcaccttcgg aaccgccgtt gcaaagatgg ttggaaagga cgtcgtcgat ctttcctttg
     6121 ttacagtccc ggtcgtcttc gccggtctga tgggcgcgat tatctggaat ctgatcacgt
     6181 ggtggcttgg gctgccatcg tcgtcgtcgc acgcgctggt cggcgggtat gccggcgcgg
     6241 cccttgcaaa aagcgggatt gcggcgctga agttcgccgg gcttgccaag attgccgcgt
     6301 tcatcgtgat tgccccgatg gttgggatga tgctggggat gctgaaccag ataatcgtga
     6361 tgtggattgt ccggcggcag ccaccgggaa agctgttccc gattttccgc aagctgcagc
     6421 tcctgtcggc cgcgatatac tcgttcagcc acggctcgaa tgacgcacag aagaccatgg
     6481 gcataatttt cgcgctgctg gttgcttccg gtctgccgcc ggccatgtgg taccaggatc
     6541 cgacgaatcc agagcatatc cctgtctgga taatccttat ctgccatgcc ttcatcgcct
     6601 tcggcacgct gttcggcgga ttcaggatcg tcaagaccat gggaagcaaa ttgacgaagc
     6661 tcgacccgat gcacggcttc tgcgccgaga caggcggcgg tgtcacgatt ctggccgtca
     6721 gcgccctggg cattccggtt tcaaccaccc acacgataac cggcgcgatc atcggcgtcg
     6781 ggactgtaag tggcttgcgc agcgttcgct ggataatggc gaaaaagatt gtctgggcct
     6841 ggatcttcac ggtgccgatg gccggccttt tcggttggct gacgttcagg ctgatggcga
     6901 tgctgggtca gggctagccg tcaacttcgg gttacaaatt ttctgttcac ttccgtcaca
     6961 tcgctggtga ctgtcagggc gtccggcctt atactcccgc ccggaatgtc cgaggtcgcc
     7021 agtggatttc gccagcatat tccagcatgt tgatcctctt gtaattatta cgcttattgc
     7081 tgccattgcg tttgactacg tcaacggttt ccacgacgcc gcgaattcga tcgccacggt
     7141 tgtggccacc cgggtactga gtccgcgatt cgcagtcgcc tgggcggcct tcttcaattt
     7201 cgcggcgctt tttgtggtcg gaaccgcagt tgccaagatg gtgggcaagg atgtcgtcga
     7261 tctttcgtac gtcacgaccg cagtcatctt ttcggggttg acgggcgcga tcgtgtggaa
     7321 cttgatcaca tggtggttcg gcctcccttc gtcatcatcg catgcacttg tcggcggtta
     7381 tgccggcgcg gcgcttgccc gaagcggcat tgaagcattg aagctggccg gtatcgggaa
     7441 gattgctgcc ttcatcgtca tcgccccgat tatcggaatg atccttggca tggcaaacca
     7501 gatcatcgtg acgtggattg tgcgcaagat gccgccaggc aagttgtacc cgctgtttcg
     7561 aaagcttcag cttctttcct cggccgtgta ttccttcagc catgggtcga acgacgccca
     7621 gaagacgatg ggaatcatct tcgctcttct tctggcatcc ggcattccgg gcaatgtctg
     7681 gggacacgac ccggttcacc ccgactccat tcccctgtgg atcgaggttc tctgctacgc
     7741 ggcgatcgcg gccgggacgc tttccggcgg acttcgaatc gtcaagacga tgggaaccag
     7801 gctgacaaaa ctggatccga tgcacggatt ctcagccgaa accggcggcg gcgtgacaat
     7861 ccttggcgtc agcttcctgg gaattccggt ctcaaccacc cacacgataa ccggcgccat
     7921 catcggggtc gggatggtgg gcggcctgcg cagcgtaaga tggatcctcg ccaggaagat
     7981 cctgtgggcc tggctgttca cgattccggc ttccggcctt ttcgggtggg gtatttaccg
     8041 ccttatgtcg ctattcggcc ataattgaat catcccggat aatttccatc ctattgacca
     8101 gcccgattac agtgtttagc ttcattggat gtggacgccc ggcttggcat ccgcgttccg
     8161 gttcgctttg ataatccggg ggaaatcatg acggttaata atgacaaggg gttgatcgga
     8221 aaggttcttt cggcggtggt cgtctttggc gccgccgttg ccggaatcgg gtcggccggg
     8281 tgtgccacga cgaccgaaag gttgaatctg caaccaccaa ccacggttgc taaggtcgat
     8341 ctgaacaggt acctgggcaa gtggtacgac atcgccagct tcccgcagtg gttccagaag
     8401 ggctgtacgg ccaccacggc cgagtacagc cttaaggaca acggggaaat caaggtggtc
     8461 aacagttgcc gcaagggcag ccttgacggc aagcagaagg tttcggtcgg acgggcaagg
     8521 gttgttgaca aagacaccaa ctcgaaactt gaagtcagct ttttcgggcc gttctggggg
     8581 aactactgga tccttgatct tggtgaaaac tatgagtatt cggttgttgg cgatcccagc
     8641 agggattatc tgtggattct ttcccgaaca ccccagatgg acggcgaagt ctatcgcggc
     8701 atcgtcgaac ggctgaaggc ccagggatat gaacttcatc ggctgcagaa gaccttgcag
     8761 ccaggcgata tgtagcgatc ctgggggtgt ccagatgaga ctctggtcga ttcatccaag
     8821 gtttcttgac accagggcca tcgttgcagt gtggcgcgag ggtcttctgg cgcgcgcggt
     8881 tctgtccggc aagactagag gctatctgaa tcacccccag cttcggcgct ttcgcgatca
     8941 gccgaatccg atttccgccc ttgacgccta tctttcggag atcgtggtcg aggccgaatc
     9001 gcgcggctac gatttcgacc gcagcaagat tgtcttcgga cgggcgccaa gcccgatcat
     9061 ggacgtaacg gcgggccagc ttttgtgcga gtggattcat cttaaccgca agcttgcggt
     9121 tcgcgacccg gtgcgactgg tgcttcagtt cgcaaggaat ccggcggcgt cgccgttctt
     9181 caggctggtg gccggcccga gggccgactg ggagaaggcg gctgattgcg gtatgtcggg
     9241 atccgcaaca gcttgacggt aacccccgtt ccgatggcga aaagcacggc gccgacccac
     9301 agcagcctgt gcggcataac gaagaccacg gaagtaatca acgtaatcca tatcagagaa
     9361 atgctgacga tttttgatgc cagcggtatg cccaggccgt cacggtaacg ccgcaggtat
     9421 tccccgaaca cccggttttc atgcatccat cggtacagtc tgtcgctgct gcgcaggaat
     9481 gccgccgcgg ccagcagcag gaagggcgtg gtcggcagaa gcggcagcag cacgccaatc
     9541 ccggcaagta ccaggcacgc gaccccggcg ataatcagta acgccttttt gagtttcacg
     9601 gcaccgcctt tgcgccaggc atgtgaggtc gttccggctg acagaccggc agcggcctgg
     9661 gcttgccttt gcatcaggca gaagatgcgt tgggatgggc ctgctgtcaa caaatagctt
     9721 gcaaaaatcc ggataaccgg ataaacgaat atgcggttgt ctcgaggtat ccgcatgaag
     9781 gatgtggctg ctttcttcaa ggtgctgtcc gaccctgcca gggttcagat cctgtggctg
     9841 ctgttcaaca attctgaact ttgcgtctgc gacatcatgg gtaccctccg aattacccag
     9901 tccaaggcgt cccggcacct ggcgaccctt cggcatgccg ggcttgttac cgacaggcgc
     9961 gacggggcgt ggtcctacta ctcgctcaaa cagccgcggg acgtccttga ggctgaggtg
    10021 attgggattc tgaagtccag gctggcgctg cttccggatg ccgggtcgct tcttgtcgca
    10081 ctggggcatt ggcttgacgt gaaggctggt cggcagtcct gctgttccct ttcaggtggt
    10141 ggtgatgagt aaggcagacg atgtcaggaa gatggtttcg gagtcctatt ccgcggcggt
    10201 gaaggcggca cttgcccgtc gcaaggcgaa acaggcgaaa tccgccccct cgaagtcatg
    10261 ttgctgcggc ggcgccccga agatcagtga acagtccctg gccgaacttg ccggatacag
    10321 ccgcgaggca ctcgccggcc tgccgccgga tgccgtgacc agttccttcg gctgcgggaa
    10381 tccggtcgcg ctggctgcga ttcgcgaagg cgatacggtt ctggatctgg gatccggcgc
    10441 gggaatcgac ctgctgctgg ccgccggcct ggccggtccg tcgggccgcg tcatcggcgt
    10501 tgatatgacg cccgcgatga tcgagcgggc ccgggccaac gcggaaaagg ccggattgaa
    10561 aaacgtcgag gtgcgccagg gcatcattga agatttgccg gtcgaatcgg gaaccgtcga
    10621 tctggtgata tcgaactgcg tcatcaatct ttcccccgaa aaaaatcggg tcttcgccga
    10681 actggcgcgt gtcctgaagc cgggcggccg gatctcgatt tcggacattt gcgccgaaat
    10741 gatgccggac tgggcgatcc aggccgggca cctttattcg gcctgcattt cgggagctat
    10801 tccggaagcc gcctacattc agggcctggt cgaggcgggc ctggaagatg taaaggttac
    10861 cgaccgtctg gtttatgatg cccagcagct ttccgggctt cttggttcgg tcgaatcccg
    10921 tggttgctgc ggcggcagtt cggctctgac tccggcccag cttgaaaggg ccgtggccga
    10981 gcttgtcggc acaatcgcca gcctgcgttt cgtcggcatg aagccagtcg tctgaccgaa
    11041 agggggccca atgaaaggca tgcttttcct gtgcgttgcc aattccgcgc gcagtcagat
    11101 cgcggaaggc cttgcgcgtt cgatggttcc acccggcgtg aatgtcctgt cggcgggatc
    11161 aagtccgacc ttcgtgcgcc ctgaagcaat cgccgtgctg aaggaaatcg gaatcgacat
    11221 ttccggccag cgttcgaagt cggttacgga aattcccccg gatcttgtcg ataccgtcat
    11281 caccctttgc ggcgagcagg aatgtccgct gttcctgggt cgtgctaccc gcctgcactg
    11341 gggactggaa gatccggccg ccgtccaggg cagcgaagaa ggtcggctga acgccttccg
    11401 aaggacgcgc gatgaactga tatcaagact ggcagaattt attgggcagg ggggggtgaa
    11461 gtgaacgatt tgaaggaacg taccaagctg attcttattg tggcagcttt cgcggcactc
    11521 tggtttctgc cggtcgagaa cgttgtcttc atgggggcaa tcagggagag tctggcgctg
    11581 gccaagtggt atgcgcgtga acacgtcatc ctttgtctga taccggcgtt tttcatcgcc
    11641 ggcgcgatca gcgtgttcgt cagccagggc gcagtcatga agtatctggg gccgaaggcc
    11701 aacaaggcgc tggcctacgg tgtcgcggct gtttcgggca cgattctggc cgtgtgttcg
    11761 tgcaccgtcc tgccgctgtt ttccagtatc cacaagcgcg gagcagggct tgggccggca
    11821 atcgccttcc tgtattccgg cccggcaatc aatgttctgg ccatcatcat gacggcccga
    11881 atcctggggc cggaactcgg cattgccagg gcagtcggcg cggttgtctt cgccgtcgtc
    11941 atcggtttcc tgatgcatct gatattccgg aaggaagagg ccgaacgcca gtcgtccggt
    12001 gtcgtgataa tcgacgacca ggaaaagacg cgtccgcttt ggaaggacgg tctgtatttc
    12061 gcggcgatgg tcggcatcct ggtctttgca aactggggca agccagcgga cggtgacggt
    12121 ggcgtctggg cggccgttca ctccgtaaag tggattgtca ccggcgtatt cctggtcggg
    12181 ttgattgcgt caatcgtcgc ctggttcagg aaggacgaac tggcgacgtg ggttgattcc
    12241 agttggacct tcgccaagca gatactgccc ctgttgtttg gcggcgtcct ggtcgccggc
    12301 ttcctcctgg gcgggcccgg gggctcggac aagggcctga ttcccaacgc ctggatccag
    12361 gggcttgtcg gtggaaattc cctgctggcg aacctttttg cttccatggt gggcgccttc
    12421 atgtactttg ccacgctgac cgaagtgccg attctcgagg ggcttattaa gtccgggatg
    12481 gggcaggggc cggcgctggc gttgctgctg gccggcccgt ccctttccct gcccagcatg
    12541 ctggtcatcc gcagtgtcct tggaacgaag aagacgcttg tttacgtcgg gcttgttgtt
    12601 gttctttcga cagtcgcggg ctggacatat ggtttaatcg ttcatgcagc ggcatgacgt
    12661 tttgttggtc agcttttcaa tggagttgga tcatgacagt aaaagtactt ggaaccggtt
    12721 gtccgaagtg caagaaattg tatgccgaag ccgagaaggc cattgctgaa tccgggcttt
    12781 cagttggaat cgagaaggtt gaaaagatca acgaaatcat ggattacggc gtgatgatga
    12841 caccagccct ggtggttggc gatcaggtca aatcgaccgg ccgcattccg gcgacggctg
    12901 aaatcgttgc ctggatcgtt gaagccgcga agacggcgtg acgcagttac gcgaaggatg
    12961 aacaccatgc agaatccgac ttgcaggttt tttgttccaa ctctcctttc tttgatcctg
    13021 gtttccgctg cttcgtgcgg cagttccggc ccgaccgaca ggaacgcggc ggtcgcggca
    13081 tcttccggtc aggtctccac ggtacccgcc tcggcgccgg cacccgtcct gcagcgtgaa
    13141 aggattgtcg cctattattt ccacggtgac cgccgctgtc ggacatgcct tggaatccag
    13201 gaaaccatcg aacggacgat tgctgaaagg tttcctgccg aaaccgcttc cggcaggctt
    13261 gaataccgcg aagtgaattt cgagcaggaa cagaacaagc acttcgtcca ggaattccag
    13321 ctttcgttcg ggacgatgat tgtcgcgaag gtcgatgacg acaagatcct tgagtgggaa
    13381 aactgcggca aggtctggga tttcggccat gagccgcccc tccttgtgga ctatgtggcc
    13441 gagcgcatca acgcatatct ctcgaagctg aaggcggaat aatggattcg atgctatttg
    13501 cggtcggcag tgcgctgtgg ctgggcatca tgacatcgat cagcccgtgt cccctggcga
    13561 ccaatatcgc cgcgatatcc tacgtcggca agcgggtgga tcgtcccggc ctggtgatgc
    13621 tgtcgggtct gctgtacatg ctcgggcgga tgctttccta tttcgtcgtg gcgctggttg
    13681 tcgtgaaaag c
//
LOCUS       DAOPPA010000017        13484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5332_length_13484_cov_4.478144, whole genome shotgun sequence.
ACCESSION   DAOPPA010000017 DAOPPA010000000
VERSION     DAOPPA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_13484_cov_4.478144"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKH54_01160"
     CDS             complement(<1..1036)
                     /locus_tag="PKH54_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98522.1"
                     /translation="MTLNITELESVIPEIQLAVRGSILTGVESPDQWTCILSFSSVDG
                     GPVKRVFFSRRPRLTRLNLLPDGWTSPAQNIGRRSIEAVKFVDAVSRALKGAEAGPMR
                     TRFRDRVVSMEMYLPKDSGRSDGSDDQPVRMFLLFECTGMHANLFLLDERQVIQAVFG
                     RSTSRRRPLDVGCRYQQPAEIEGPRPVGRRVDVMRFVRSDMSLGHQISEMYDPLDQVD
                     DRKRELSSLAEELSFVVEERRKVAESVRRDLAEPVRPGRPADLPERLRERLDRVDSEV
                     RELQELLHGVRKGDVSVFDRATELVTEYRAKIQEIERRRAERPARPDWRPREGGYQRR
                     DDSPRPPREGG"
     gene            1159..2697
                     /locus_tag="PKH54_01165"
     CDS             1159..2697
                     /locus_tag="PKH54_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98523.1"
                     /translation="MRKRIATVSGRRVASWPTFLLATATLVSAPWCAGQESESDVGGD
                     TDTVITDAGPDIPNLDPVEVDEDFRVIYGYAATAGDRPGDLGISNPLDGEGQPGFPAW
                     KDNFQVNARILAPLGLDCSLGCVTEPALNFLLVATTTGVGTPGQDARLIAIDETGQPT
                     IVTEQPIPAVRQAVFSGRSLYVSRVKNDCESKDGGIRTCYRFGRIDLDDPVFEIQELF
                     EFPGPDLAGSSNYTGRFRLGADGVTLVLQNLELESLTFYLFNEKAGLRKAGVPVCQAI
                     DLQGKCMYSQDAPQLSDDIPAILSADGRTLLFAHVFDARELRLAKMDVESGSIESVTL
                     VRTPSRYASNACYNLNRDWPYTSIMQPLLLSRDGRELIVAAASTCDGAPGKTWTNILA
                     FSVSDIGTDANLTDIFRKITDFPKVGAPEMISVSPDSMSLSPSGRFLSFVGTAIRDSS
                     GQPLTAATQQHLIDREVFVTSTESLKRPIQISGNLDYRATFTMCSVANPGFSAFTDPL
                     PVED"
     gene            complement(2736..3428)
                     /locus_tag="PKH54_01170"
     CDS             complement(2736..3428)
                     /locus_tag="PKH54_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOC98524.1"
                     /translation="MRGPAVIFDLDDTLYPSNSFVAGGIRAVAVLLRDDGFTDPDPCD
                     VLMEVLETEGPFSLFDSAFARLGVRRTPELVSRLVAAFRAHSPSISPYPGIVDLLCSL
                     RNHGVGLGLVTDGYPQVQRSKWTALGLGHLFDAVVFCRDIDGGDHPKPDPFPFARAHH
                     LLGEPDSVVFVGDNPSADFPAPDAFGWRTVRVCWPEATHILDGDTVINRPVARNAAEL
                     RAILSRILDFPS"
     gene            3552..4613
                     /gene="ispG"
                     /locus_tag="PKH54_01175"
                     /gene_synonym="gcpE"
     CDS             3552..4613
                     /gene="ispG"
                     /locus_tag="PKH54_01175"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC98525.1"
                     /translation="MGSTRTVNIGGIEMGGGAPISIQSMCNADPYDHDAVTAQVRQLH
                     AAGCDIVRISVPRLDAVPAAIRVREMTAGIPLVADVHFDHRVAIAMCGHIDKIRINPG
                     TLKGKAALRETAAALLDTGTPVRIGINSGSLPEDLAAATGDPATVLVEAATRAVSDMR
                     ELGVEDIVVSVKSSSPTVTMLACELAASRLDVPLHVGVTESGTPASGAVRSAAVIAAL
                     LSRGIGDTIRVSLAGSPLPEVKVARQILESIGLRKPGIQVRACPTCGRACLDTESIAA
                     GIEAALEGLDASREPPELVIAVMGCVVNGPGEAAGADAAIVGTPGGAALFIKGRVAMK
                     GSVDQALQQLLCWIHRPQD"
     gene            complement(4595..7807)
                     /locus_tag="PKH54_01180"
     CDS             complement(4595..7807)
                     /locus_tag="PKH54_01180"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC98526.1"
                     /translation="MRTGSSGMLPVVAMACLFVSSCGSHVEKADIGGSCREDSDCQSA
                     LCFADVCIDPLADQDWDGLSAGDEVEIGLDPFHPDSDRDGIPDGLEVGPELATPADYD
                     GDGVIDALESAFSDSDGDCLPDQYDPLDALGPAGSSQMVPVLCMTAGVCGAAMNTIVA
                     TCDGSKVTCSYEGVPGWNAAEWMCDGKDNNCDGRTDEGFLHEGLSIRLPCLGAGSCGP
                     GIVECDDSGFSTRCSSNPGASGDRSMPEVCDGLDNDCDGLTDNGMSWKGVPLGERCDG
                     EGECGIGKVECLADRTASCSTMKGGSHEQIQEESCDNLDNDCDGRTDETFFGNASELC
                     VPRGVCALFPNMISLKCEQGILKCDFSAVPGYSGSSEMFCDGQDDDCDGLVDEELSFA
                     VTEPGAVIRLPGQSCGSGACAGGVVTCSPSSMSGMCSTTPLAAPEKCDGIDDDCDGYI
                     DNGFALSWKPALETIMDREPQALVSSRMAVVEYSLEDPPEPGEPPAGIYVCGGAVGSP
                     ENGSSGISRRCWLYSFDSHRFDRLPDGPEVDSGAMVHDRAGGSLLMVGRLFASGEVAV
                     FRMNGIDFGWSRLPWELESGEVLAAGVDPVENTLNILTNGDSGLLVSMVGLSDGSVLT
                     VQAGERVSAGSFAAGSPSGGFVLFHACNELAGTVVEHVSPDGQVNGLPLAGQSQCMSF
                     GAAVMPIPGRIVAIEGRALDGSVQATREFLLGDDLWHHERITVPGGQGSVMHPAMASS
                     GDTVYAFSGEYGDGGALRRVIRLDRESTEWLVDSFVSGPSPRAGGVAFVSRLTAAAYF
                     IGGYSDEPGGPQPVQDVWKIDLVNPRIGQIAVSGEPGAVIGAAAAINDSGQTAYIFGG
                     LDSPPGPDSRPVSRFLRFNCIQSRFDSIQSGEGGPTARYGHSLVWAGDRLLLYGGLND
                     TGFLSDLWSWAPDEGWIFIGYFELSYGHQAFWDNPGKRMLVVGGRPGGGLRAYYPATS
                     TWKSLLSGVLFDSYEGHGWFDEQSGSVLYLSSTSSDAVVVTFLEDGLVDQVIPGLQPD
                     LIDAFVVYDPFGRRAMMLGGRDPSNGTVGTVSQIDQSCGR"
     gene            8041..9042
                     /locus_tag="PKH54_01185"
     CDS             8041..9042
                     /locus_tag="PKH54_01185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC98527.1"
                     /translation="MKEDLLRFETGDHENGARLDMVIAARFPAISRSLAQKLIKNGAA
                     LVNGRQRPAGFRLAAGMQVELTPPPPSDHAGPAPEHGGPELEILFEDEFLMVINKRAG
                     LVVHPGAGHGSGTLVNQLLASGRTFSNFGGPDRPGIVHRLDKDTSGVMIVARDNHTHE
                     LLGDMFRERTINKTYLAVVLGPGIDDQGLIETSFGRRTGDRRQFTGRVRDGRDAVTGY
                     RTLMRANLCALVAAKPMTGRTHQIRVHLSEAGHPIVGDRVYGRGWPRRGSRPESEVAA
                     LEMIKRHALHAWNIRFAHPVTGQQMDITAPLPGDFRRMLDGLFGDQWKDLLSREAAR"
     gene            9153..9992
                     /locus_tag="PKH54_01190"
     CDS             9153..9992
                     /locus_tag="PKH54_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98528.1"
                     /translation="MEEQVKTFLKAIKAAANAGKSVEEFSREHRKGNLASSIDMAERA
                     LRAIGDIAGLETGAAAMKAAIAATLSESRSRLDREKALLAGAVAKRLNEEGFKVSGNL
                     PTLTAGPFSLEFTFGSKGLCTLWLGPGKYRLAAASLDPEDICAQIREQNGRLFPDSFD
                     EPSFLAELEKAYRLAVIRTGAESGGRVPVTSLIPEIAFMRQKPAFLIDPRKETFRSYG
                     RVEFAADLSRLRNRIIRGMELRLDVATMSQTRNAGDHLWVPRPGILEGVNFATLRFVK
                     VTA"
     gene            10024..11313
                     /locus_tag="PKH54_01195"
     CDS             10024..11313
                     /locus_tag="PKH54_01195"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOC98529.1"
                     /translation="MVDNLTARQARHIIEVVGSSGTPPEWGFQFFSSGLDGYLKVIED
                     DYLKSFVREGGSSFKVVVGTYGGGKTHFLYSVRELAWRHDYLVTYCPLSAESSPFHRL
                     DMVYKSIAVNLMRPLTPDELLTGGERGLGAFIKAVYHDMKTAIAEEEGIAGEQLEDRI
                     RAIAADSVRGCENPNFGKAVRLAIEALTRNDQDQFDAMLQYLTVDGWDRLSHKAGGVL
                     QPIDRSQAFATIRSLVTWVRNLRFGGIIILFDEAEQAGSMTSKQKELMLANLREMVDQ
                     CGASSFANTMVFYAIPNEQFLTEGRSPAYEALRQRIQTVFDYTNPTGVKVRLDRIARD
                     PKTLMNDIGRKLCGVYENAYQFRMPADQTRNLVRVLADATAERAFGDIGYKRMFVQAV
                     VRAMHALRFEPDTVIDEEFATRMLSPGEDADPDDIYE"
     gene            11306..12802
                     /locus_tag="PKH54_01200"
     CDS             11306..12802
                     /locus_tag="PKH54_01200"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOC98530.1"
                     /translation="MNRFEKGDAVTHATYGDGTVLGSRIGGFEFRVNFGVYSLWIPAR
                     ELVRAGEGLKLVEDEAPAVRSTAPSSTSLDAILRLLGGQAPASEEPAGARKHRAPVYR
                     MLPENRPVSASMAIEAFRLGIVPSSSIGEWTVGRKDEVGQLMEFLHDHAEGAALVEGA
                     YGAGKSHLLRFLAGEAEQLGYAVAMAGFDPSEASAAFPKKAYRHLVTGFKAVVDDRVL
                     NFRGFWREVASRPGWRDVLGDHWLFASVLERLQEEEPDERPGLTEEEWDFIEARGEGR
                     IGRKKTLYDYSTCANIYCNILSATARAASEVLGMMGLLVLLDEAEVAGSVMYRYQAQR
                     GMNFFRGLVLTANDDDVLMDEELIRAETIQGAVSGLIYSAHHPVRYTTGIPGALKVAF
                     ALTPGTLQDEFSRTRETITRVQVDVLSMDQLRELFRRICRTYDTTYGVKLEKKRQDRL
                     FRLLYTADRIQSTRTFIKSVIEALDYVRFYPDGDVEQMVVSGGEGDAI"
     gene            12799..>13484
                     /locus_tag="PKH54_01205"
     CDS             12799..>13484
                     /locus_tag="PKH54_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC98531.1"
                     /translation="MKPGIDRFSRMDAARSLPRAFPAFLARFGNLTGIQELAIPVIAS
                     GRDAVVVAPAASGKTEAVLAPLFEEMAAIEDPRPQVGILYIVPTRALANDIENRVREP
                     ASAMKMSSRVRTGDRPSPVGRDHTDLLITTPESFDSLLCRHPAAFDNLHAIVIDEAHL
                     MDGTARGDQLRILIRRIQMKRSAARPRLIAVTATVGDPAGLGTRLFGERPEVVRDDRR
                     RPLDATVSPD"
BASE COUNT         2862 a         4149 c         4038 g         2435 t
ORIGIN      
        1 aaccgccttc cctgggtgga cgcggcgagt cgtcgcggcg ctggtatccg ccttcacgcg
       61 gcctccagtc gggacgggcc ggacgttcag ccctgcgacg ctcgatttcc tggatcttcg
      121 cgcggtactc tgtaacaagt tccgtcgcgc gatcaaaaac gctgacgtcg cccttgcgta
      181 cgccatgaag cagttcctga agctcgcgca cctctgaatc gacgcggtca agccgttccc
      241 gcagacgctc tggaagatcc gcaggccgcc ccggacgaac cggctcggcc agatccctgc
      301 gaaccgactc cgcaaccttg cgcctttcct cgaccacgaa cgacagttcc tcggcaagcg
      361 aggaaagctc gcgcttgcgg tcgtcaacct gatccagggg atcgtacatt tcgcttatct
      421 gatgtcccag ggacatgtcc gaccttacga agcgcatcac atcgacacgc cgcccgacag
      481 gcctgggccc ttcgatttcg gcgggctgct ggtacctgca gccgacgtcc aggggacgcc
      541 tgcgcgacgt cgaacgcccg aacactgcct gaatgacctg cctctcatcg agaagaaaca
      601 gattcgcatg cattccggtg cattcgaaaa gcaggaacat cctgaccggc tggtcatccg
      661 acccgtccga acggcccgaa tccttcggca gatacatttc catcgaaacg acccggtcac
      721 ggaaacgggt cctcatcgga cccgcctcgg cgcccttcaa tgcacgggag acggcatcga
      781 cgaactttac ggcctcgatt gaccggcgac cgatgttctg cgcgggcgag gtccagccgt
      841 ccggaagcag gtttaacctg gtcagcctgg gacgccttga gaagaagacc cgcttcacgg
      901 gcccgccgtc aacgctggag aaggaaagaa tgcaggtcca ttgatcggga ctttcgacac
      961 ccgtaagaat ggaaccgcga acggcaagtt gaatctccgg gataaccgat tcaagttcag
     1021 tgatgttaag ggtcataaac tccagtgcca ggcgcaaggc caaaggggaa aaagggtcga
     1081 tattatatca cctttcagat ggaactcaaa tggcaaacaa cggtaagatt gaccttgaat
     1141 tgcgcggagc atgaccggat gaggaagcgc attgccaccg tttcgggccg cagggtcgcg
     1201 tcatggccga cattcctgct tgccacggcc actcttgttt cggccccgtg gtgcgccggg
     1261 caggaatcgg aaagcgatgt cggcggcgat acggacaccg tgattaccga cgccggccct
     1321 gacattccga accttgatcc ggtcgaggtc gatgaagact ttcgcgttat ctacggatac
     1381 gcggccacgg cgggcgacag gcccggcgac ctcgggattt ccaacccgct cgacggcgag
     1441 gggcagcccg gcttcccggc atggaaggac aacttccagg ttaacgcaag gatcctcgcc
     1501 cctctgggac tcgactgctc cctgggctgc gtgacggaac ccgccctcaa cttcctgctc
     1561 gtggcgacga caacgggcgt cggcacaccg ggacaggacg ccaggcttat cgccatcgat
     1621 gagacaggac aaccgacaat cgttacggag cagccgatcc cggccgttcg ccaggcagtc
     1681 ttttcgggaa ggtcacttta cgtttccagg gtaaaaaatg attgcgaatc aaaggatggc
     1741 ggaatacgga cctgctatcg cttcggtcgg atcgaccttg atgatcccgt ttttgaaatc
     1801 caggagttgt ttgagttccc ggggccggat cttgcgggca gctccaacta caccggaagg
     1861 ttcaggctcg gcgcggacgg tgtcacgctg gttctgcaga atctggaact tgaatccctg
     1921 acattttacc tgttcaacga aaaggcaggc cttagaaagg ccggcgtacc ggtgtgccag
     1981 gcgattgacc ttcagggcaa gtgcatgtat tcccaggatg ccccccagct ttcggacgac
     2041 atcccggcga tcctttcagc ggatgggcga accctgctgt tcgcccacgt cttcgacgcg
     2101 cgcgaactcc ggctggcgaa gatggacgtg gaaagtggtt caattgaatc ggtgacgctg
     2161 gtcaggactc cgtcgcgata cgcttcaaac gcatgttaca acctgaacag ggactggccg
     2221 tacacctcga tcatgcagcc cctgctgctt tcccgcgacg gccgggaact gatcgtcgcc
     2281 gcggcatcga cctgtgacgg cgcccccggg aagacatgga ccaacatcct ggcattctcc
     2341 gtcagcgaca tcggcacgga cgcgaatctt acggacatat tcagaaagat cacggacttt
     2401 ccaaaggttg gggcgccgga aatgatttcg gtgtcgccgg acagcatgtc gctttccccg
     2461 tccgggcgct tcctgtcatt tgtcgggacg gccatccgcg attcatcagg ccagccgctt
     2521 accgccgcga cccagcagca cctgattgac cgggaggttt tcgtgacatc gaccgaaagc
     2581 ctcaaaagac caatccagat ttccggcaac cttgactatc gggcaacctt caccatgtgc
     2641 tccgtcgcga atcccggatt ctccgcgttc accgatccat tgccggtcga ggactgattg
     2701 ccattccaca accttttcaa gtcaaccgat ccggatcagg acggaaaatc gaggattctt
     2761 gaaaggatag cccgcagctc ggccgcattt cgggcaaccg gcctgtttat tacggtgtcc
     2821 ccgtcaagga tgtgcgtcgc ttcgggccag cacaccctta cggtgcgcca gccaaaagca
     2881 tcgggagccg gaaaatcggc cgacggattg tcaccgacga agacaacgga atcaggttcc
     2941 ccgagaaggt ggtgggccct ggcgaaaggg aagggatcag gctttggatg gtcgcctccg
     3001 tcgatgtccc ggcaaaaaac caccgcgtca aagaggtgcc cgagcccaag agcggtccac
     3061 ttggatctct ggacctgggg ataaccgtcg gtaacaagcc caaggccaac gccgtgattt
     3121 cgaagtgagc aaagcaggtc aacgatgccg gggtacggcg atatggacgg actgtgggca
     3181 cgaaacgccg cgaccagcct ggaaaccagt tcaggcgtcc ttcggactcc aagacgtgca
     3241 aatgccgagt cgaaaaggga aaatggacct tcggtctcga gaacctccat caggacatcg
     3301 caaggatcgg gatcggtgaa tccgtcatcc cggagcagga cagcaaccgc gcgaatgccg
     3361 ccggccacga atgaattcga gggatagagc gtgtcgtcaa ggtcgaagat aaccgcggga
     3421 ccgcgcatga aagcccccga tgatgaggca ccggtcagca tattccagtc gggggcgggc
     3481 acgcaaccgg caaaatgata tacgattggc cgaaccgatc atgcgtcgga ctgacgccac
     3541 gaggtcttca gatgggttcg acaaggacgg tgaacatcgg cggaattgag atgggcgggg
     3601 gggccccgat atcgatccag agcatgtgca acgccgatcc ctatgaccat gacgcggtaa
     3661 ccgctcaggt tcgtcagttg cacgctgccg ggtgcgacat tgtccggata tcggttccgc
     3721 gccttgacgc agtgccggcg gccatcaggg tcagggaaat gacggccggc attcctctgg
     3781 tggcggatgt gcatttcgat cacagggtcg ccatcgccat gtgcggccac atcgacaaga
     3841 tcagaatcaa tccgggaacc ctgaagggga aagcggccct gcgcgagacg gcggcggccc
     3901 ttctggacac gggaaccccc gtaagaatcg ggatcaactc gggctccctt cccgaggacc
     3961 tggcggcggc aaccggagat ccggcgaccg tgctggtcga agctgccacg cgggccgttt
     4021 cggacatgcg ggaactgggg gttgaggaca tcgttgtatc ggtgaagtcc agttcgccga
     4081 cagtaaccat gcttgcttgc gaacttgcgg catcccggct ggacgtcccg cttcatgtag
     4141 gcgtcacgga atccggcaca ccggcctcgg gggcggttcg atccgccgcg gtcatcgccg
     4201 ccctgctttc ccggggaatc ggggacacga tcagggtttc gcttgcaggt tcgccattac
     4261 ccgaagtcaa ggtcgccagg cagattctgg aatcaatcgg cctccggaaa ccgggcattc
     4321 aggttcgggc atgcccgacc tgcggcaggg catgcctgga caccgaatcg atcgcggctg
     4381 gaatcgaggc ggcccttgag ggactggacg caagccgtga accgccggaa ctggttatcg
     4441 ccgtaatggg atgcgtggtg aacggtccgg gcgaagccgc aggcgccgat gcggcaatcg
     4501 ttggaactcc gggcggcgcc gcgcttttca tcaagggccg ggtcgcgatg aagggttcgg
     4561 tggatcaggc tttgcagcaa ctgctgtgct ggattcatcg cccgcaggac tgatcgattt
     4621 gcgaaacggt tccgacggtc ccgttcgagg gatccctgcc accgagcatc atggcccggc
     4681 gcccgaacgg atcataaacc acgaacgcat cgatcaaatc cggctgcagt ccggggatga
     4741 cctgatcgac aagcccgtct tcaaggaaag tcacgaccac cgcgtcgctg gatgtcgatg
     4801 aaagataaag cacgctgccg gattgctcgt caaaccagcc gtggccttca tacgaatcga
     4861 acagaacccc ggacagcagg cttttccacg tggatgtggc gggatagtat gcgcgaaggc
     4921 ccccacctgg cctgcccccg acgaccagca tgcgcttgcc gggattgtcc cagaacgcct
     4981 ggtggccata gctgagttcg aaatatccaa tgaagatcca gccttcgtcc ggtgcccagg
     5041 accagagatc ggaaaggaaa ccggtgtcgt tcagaccgcc gtaaagaagc aacctgtcac
     5101 cggcccagac aagcgagtgt ccataacgtg ccgtcggacc gccttccccc gactgaatcg
     5161 aatcaaacct cgactgaatg cagttgaacc gaaggaacct gctaaccggc ctggagtcgg
     5221 gccccggcgg cgaatcgagc cccccgaaaa tgtaggcggt ctggccggaa tcattgatgg
     5281 ccgcagcagc acctataacc gcccctggtt cgcccgacac cgcaatctgt ccaatccgcg
     5341 gattgaccag atcaatcttc cacacatcct ggactggctg aggcccgccc ggttcatccg
     5401 aatagccgcc aatgaaatag gccgccgccg tcaggcgcga aacgaacgca acgccgcccg
     5461 ccctgggaga tggaccgctt acgaagctgt caaccagcca ttccgtcgat tcgcgatcca
     5521 gccggatcac acgccgcagt gcccctccat caccatactc cccggaaaat gcatagacgg
     5581 tgtcgccaga ggaggccatc gcgggatgca tgacggatcc ctggccgccg ggaaccgtga
     5641 tcctttcgtg gtgccacagg tcatcgccca gcaggaattc ccttgtggcc tggaccgagc
     5701 cgtcaagtgc cctgccctca atcgcgacga tgcgtcccgg gatcggcatg accgccgcgc
     5761 cgaaggacat gcattgactc tgtcctgcaa gcggcagccc gttgacctgc ccgtccggcg
     5821 agacatgttc gaccacagtg ccggcaagct cgttgcaggc atgaaacagg acaaaccccc
     5881 cggagggcga accggcggca aaggagccgg cagagacgcg ctcgcccgcc tgaacggtca
     5941 ggacggagcc atcggacagc ccgaccatgc tcaccagaag cccggaatca ccattcgtga
     6001 ggatattcag ggtgttttca accggatcca ctccggcggc aagaacctcg ccactttcca
     6061 gttcccaggg cagcctgctc catccgaaat cgattccgtt catccggaaa acggcgactt
     6121 cgccggacgc gaacagacga cccaccatca gcaggcttcc gcccgccctg tcatgaacca
     6181 tcgcccccga atcgacttcg ggcccgtcgg gaagtcgatc gaaacgatgc gaatcaaagc
     6241 tgtacagcca gcaacgcctt gaaatcccgg acgaaccgtt ctcaggactt ccaacggccc
     6301 cgccgcatac atatatgccg gcaggcggtt cacctggttc aggcggatcc tccagggaat
     6361 actcgaccac ggccatccgc gacgaaacaa gcgcctgcgg ctccctgtcc atgatggttt
     6421 caagcgccgg cttccaactc agggcgaaac cgttgtctat gtacccgtcg cagtcgtcat
     6481 cgattccatc gcacttctcc ggcgcggcaa ggggcgtggt ggaacacatg ccggacatcg
     6541 aactcggcga gcacgtgacg accccgccgg cacaggcgcc cgaaccgcag gactggccgg
     6601 gaagccttat cacggcgcca ggttcggtca ccgcgaacga cagttcctcg tcaaccagcc
     6661 cgtcgcaatc gtcatcctgt ccatcacaga acatctcgga acttcccgaa tacccaggca
     6721 cagcactgaa gtcgcatttc aggatcccct gctcgcactt caacgaaatc atgttgggga
     6781 acagggcgca gacgccgcgg ggaacgcaca gttcggaagc gttgccgaaa aatgtctcgt
     6841 cggttcgtcc atcacagtcg ttgtcaaggt tgtcacagga ttcctcctgg atctgttcgt
     6901 gacttccacc cttcatggtt gaacaggaag ccgtcctgtc ggccaggcat tcgaccttgc
     6961 cgatgccgca ctcgccttcg ccgtcacaac gttccccgag gggcacgccc ttccacgaca
     7021 tgccgttgtc ggtcaagccg tcgcaatcgt tgtcgagacc gtcgcacacc tcgggcatgg
     7081 atctgtcgcc agaagcgccg gggtttgacg aacacctggt cgaaaagccc gagtcatcgc
     7141 actcgacgat acccgggcca caacttcctg ccccaaggca gggaagtctg atggacaagc
     7201 cttcgtgaag aaaaccttcg tcggttcgac cgtcgcagtt gttgtccttt ccgtcacaca
     7261 tccattccgc ggcgttccag cctggcacgc cttcatagct gcacgttacc ttcgagccgt
     7321 cgcaggtagc gacaatggtg ttcattgcgg ccccgcagac cccggctgtc atgcacagca
     7381 ccggcaccat ctgacttgag ccggccggcc cgagcgcatc gagaggatca tactgatccg
     7441 gaaggcagtc cccgtcagaa tccgaaaaag cggactcaag agcgtcgatg acgccgtccc
     7501 cgtcataatc cgccggagtc gcaagctcgg ggcccacttc caggccgtcg ggaatgccgt
     7561 cgcgatcact gtccgggtga aacggatcca gcccgatttc aacttcatcg cccgccgaaa
     7621 gaccgtccca atcctggtcc gccaggggat cgatgcatac gtctgcgaaa cagagggcgg
     7681 attgacaatc ggagtcttcc cggcaggagc ccccgatatc ggccttctcc acatggctgc
     7741 cgcacgacga cacaaataga cacgccatcg caacgaccgg caacatcccg gaagacccgg
     7801 ttcgcatctg gaatctccga tgcccggcga tcgacttgcg acgcccggca gacaatcccc
     7861 gcacaacatt cagaccgctt cagccggcat gatcgcccga aacgcgccgc cagacaaggg
     7921 ccggcactct ggctgacggc atggcgcgcg aaggcgtccg gcccggcccg gacggatcca
     7981 tgttacaatc cgcgacgtac tttttcactt gtccgaaaat ggacaacccg gatttggccg
     8041 atgaaagaag atctgctgcg atttgaaacg ggggatcacg aaaacggcgc aaggcttgac
     8101 atggtcatcg cggccagatt ccccgccatc tccaggtcgc tcgcccagaa attgatcaag
     8161 aacggggcgg cgctggtcaa cggaaggcaa cgaccggcag gcttcaggct tgcagccgga
     8221 atgcaggtgg aactgactcc gccgccgccg tccgaccatg ccggtcccgc tcccgaacac
     8281 ggtggcccgg aactggaaat ccttttcgag gatgaattcc tgatggtgat caacaagcgg
     8341 gccggactgg tcgttcaccc gggcgcggga cacggctcgg gaacacttgt gaaccagctc
     8401 ctggcgtccg gccgcacatt ttcgaacttc ggcgggccgg acaggccggg aatagtacac
     8461 cggctggaca aggacacttc gggcgtgatg atcgtcgcaa gggacaatca cacacacgaa
     8521 cttctcgggg acatgttccg cgagcgcaca atcaacaaga cgtacctggc cgtggtgctt
     8581 ggaccaggca tcgacgacca gggactcatc gagacatcct tcggcagacg aaccggggac
     8641 agacgccagt tcaccgggcg cgtgcgcgac ggccgcgacg cggtgaccgg ataccggacg
     8701 cttatgcgcg ccaacctgtg cgcgctcgtg gcggccaaac cgatgaccgg aagaacccat
     8761 caaataaggg ttcacctgtc cgaggccggg cacccgattg tcggtgatcg cgtctatggc
     8821 cgcggatggc cccgcagggg aagccgcccc gaatccgagg tggccgccct ggaaatgatc
     8881 aagcgtcatg cccttcatgc ctggaacatt cgcttcgccc accccgtcac cggacaacag
     8941 atggatatca ccgccccgct tccgggcgat ttccgcagga tgctggatgg actcttcggt
     9001 gatcaatgga aagacctcct gtcccgcgag gccgcgcgct gacgaagttc ccgacaggtt
     9061 acacgctttc aatcgccata aattgatttg gccttataat actgcgcggc cggggcgacc
     9121 cggatcggat gaagcaaccc cgggggaagc ccatggaaga acaggtcaag acgttcctga
     9181 aggccatcaa ggctgccgcc aacgccggaa agtcggttga ggaattctcc agggaacacc
     9241 gcaaggggaa cctggcatca tcaatcgaca tggccgaaag ggcgttgcgg gcaatcgggg
     9301 acatcgccgg gctggaaacg ggcgccgcgg caatgaaggc ggcgattgcg gcgacgctgt
     9361 cggaatcccg cagccgcctt gatcgcgaga aggcgctgct tgcgggagcg gtcgcaaaac
     9421 gcctgaacga agaaggcttc aaggtgtccg gaaaccttcc cacgctgacg gccgggcctt
     9481 tctcgctgga attcacattc ggttcaaagg gcctgtgcac cttatggctt ggacccggca
     9541 agtacaggct ggcggcggct tctctggatc ccgaagacat ctgcgcgcag atccgggaac
     9601 agaacggcag gctctttcca gattctttcg acgagccgtc tttccttgcc gaacttgaaa
     9661 aggcttaccg gctggccgtg atacgcaccg gtgccgagtc cgggggccgc gtgccggtca
     9721 cgtcgctgat cccggaaatc gcctttatgc gccagaagcc ggccttcctg attgatccgc
     9781 gaaaggagac cttcaggtcg tacggaaggg tggaattcgc ggcggatctg tcaaggctca
     9841 ggaaccggat catccgggga atggaactga ggcttgacgt ggcgacgatg tcccagacaa
     9901 ggaatgccgg ggatcaccta tgggtgccga gacctggaat ccttgagggc gttaacttcg
     9961 caacgctccg gtttgttaag gttactgcct gagtttggac gactcaggaa cgggagattt
    10021 cagatggttg acaacctgac cgccaggcag gcccgccaca ttatcgaagt ggtcggttcg
    10081 tccggaaccc cgcccgaatg ggggttccag ttcttttcct cgggccttga cgggtacctg
    10141 aaggtgatcg aggacgatta cctcaagtca ttcgtccgcg aaggcgggtc gtcgttcaaa
    10201 gtcgtcgtcg gaacgtatgg cggcggcaag acacacttcc tgtacagcgt ccgagagctt
    10261 gcctggcgtc atgactacct ggtcacatac tgcccgcttt cggcggaatc gtcgccgttc
    10321 caccgcctgg acatggttta caagtccatc gcggtcaacc tgatgcgtcc actgacgccg
    10381 gatgaactcc tgaccggcgg ggagcgcggg ctcggcgcct tcatcaaggc ggtctatcac
    10441 gacatgaaga ccgccatcgc cgaagaggaa ggcatcgccg gcgaacaact ggaagatcgc
    10501 atcagggcaa ttgcggccga ttcggttcgt ggctgcgaaa acccgaattt cgggaaggcc
    10561 gtccgacttg ccattgaagc gttgacccgc aatgaccagg atcagttcga cgcaatgctg
    10621 cagtacctta ccgtcgatgg ttgggatcgg ctgtcgcaca aggctggagg ggttctgcag
    10681 ccgattgaca gatcccaggc gttcgccacg atcagatcac tggtgacgtg ggttcggaac
    10741 ctgcgcttcg gcggcatcat catcctgttc gacgaggccg aacaggccgg ctcgatgact
    10801 tccaagcaga aggaactgat gctggccaac ctgcgcgaga tggttgatca gtgcggggct
    10861 tcctcgttcg cgaacacgat ggtcttttat gccattccaa acgagcagtt cctgaccgag
    10921 ggccgatctc cggcttacga agcgcttcgc cagagaattc agacggtatt cgactacacg
    10981 aaccccaccg gcgtcaaagt ccggcttgat cgaatcgcaa gggatccgaa gaccttgatg
    11041 aacgacatcg gaagaaaact gtgcggcgtc tatgaaaacg cctaccagtt ccgcatgccg
    11101 gccgatcaga cccgcaatct ggtaagggtt ctcgcggatg cgaccgccga acgcgccttt
    11161 ggcgacatag ggtacaagag aatgttcgtc caggccgtcg tgcgggcaat gcacgcgctg
    11221 cggttcgaac cggacaccgt gatcgacgag gaattcgcca cgcgaatgct ttcgcccggc
    11281 gaagatgccg acccggacga tatctatgaa taggttcgaa aagggcgatg ccgtgacgca
    11341 tgccacatat ggcgacggca cggtgcttgg ttcacggatt ggcggatttg aattccgtgt
    11401 caacttcggg gtttacagcc tgtggatccc ggcgcgggaa cttgtccgtg ccggtgaagg
    11461 gctgaagctc gtcgaagacg aggctccggc tgttcgatcg acggcaccct ccagcacatc
    11521 ccttgacgca atcctccgcc tgcttggggg acaggcaccc gcgagcgagg aacctgcagg
    11581 agccaggaaa catcgggctc cggtttatcg aatgctgccg gaaaaccggc cggtcagtgc
    11641 aagcatggct atcgaggcct tcaggcttgg gatcgtgcca tcttcatcga tcggggagtg
    11701 gacggtcggg cgcaaggatg aagtcgggca actgatggaa ttccttcacg accatgccga
    11761 gggcgcggcc ctggtggaag gggcctacgg cgcaggcaag agccacctgc tgagattcct
    11821 ggcgggcgaa gcggagcaac tgggctacgc ggtcgccatg gccggtttcg atccatccga
    11881 ggcatcggca gcttttccca agaaggctta ccggcatctg gtcaccggat tcaaggccgt
    11941 cgttgatgac cgggtgttga acttccgtgg attctggcgt gaagtggcgt cccggccggg
    12001 ctggcgggat gtccttggcg accactggct ttttgcaagc gtcctggagc ggctccagga
    12061 ggaggagccg gacgaaaggc caggacttac cgaggaagaa tgggacttca tcgaagcccg
    12121 cggggaaggc cggatcgggc gcaagaagac gctttacgat tactcgacct gtgccaacat
    12181 ctactgcaac atactttcgg caactgccag ggccgcatcg gaagtccttg gaatgatggg
    12241 cctgctggtt cttctggacg aggcggaagt ggccggaagc gtaatgtacc gataccaggc
    12301 ccagcgcgga atgaacttct tccgcgggct tgtccttaca gccaacgatg acgacgtact
    12361 catggacgag gaactcattc gtgccgaaac aatccagggc gcggtatccg gcctgatata
    12421 ctcggcacat caccccgtca gatacaccac cggaattccc ggtgccctga aggtcgcgtt
    12481 cgcgcttact ccgggcacgc tccaggatga attcagccga accagggaaa cgattacaag
    12541 ggtccaggtc gatgttcttt caatggatca gcttcgcgag cttttccgca ggatatgccg
    12601 cacttatgac accacgtacg gcgtgaaact ggagaagaag cgacaggaca ggctgttcag
    12661 gctgctgtac acggccgacc ggatacaatc gaccaggacg ttcatcaagt ccgtgatcga
    12721 agccctcgac tacgtccgct tctatcccga tggcgacgtg gaacagatgg tcgtttccgg
    12781 tggcgaaggc gacgcgattt gaaaccgggc atcgacagat tcagccggat ggatgccgcc
    12841 agatcccttc cgcgcgcgtt tccggcattt ctggcccgct tcggcaacct taccgggatc
    12901 caggaacttg cgattccggt cattgcctcg gggcgggatg ccgtcgttgt cgcgcccgcc
    12961 gcttccggca agaccgaggc cgtgctcgcc ccgcttttcg aggaaatggc cgccattgaa
    13021 gatccccgtc cgcaggttgg cattctgtac atcgttccca ccagggcgct ggctaacgac
    13081 atcgaaaacc gcgttcgcga gccggcctcg gcaatgaaga tgagcagccg ggtcaggacc
    13141 ggcgaccgcc cttcaccggt tggaagggat cacaccgacc tcctgattac gacgccggag
    13201 tctttcgatt cgctgctttg ccgccatccg gcggctttcg acaaccttca cgcgatcgtc
    13261 atcgacgagg cgcacctgat ggacggtacc gcgcgcggcg accagttgcg gatactgatt
    13321 cgacggattc agatgaaacg gtcggcggcc aggccccggc tgattgccgt aacggcgacc
    13381 gtcggggatc ccgccggcct gggaacgcga cttttcggtg aaaggcccga ggtcgttcgg
    13441 gatgacagaa gaaggcctct ggatgccacg gtgtcccccg acat
//
LOCUS       DAOPPA010000018        13117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5520_length_13117_cov_8.532231, whole genome shotgun sequence.
ACCESSION   DAOPPA010000018 DAOPPA010000000
VERSION     DAOPPA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5520_length_13117_cov_8.532231"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1091)
                     /locus_tag="PKH54_01210"
     CDS             complement(132..1091)
                     /locus_tag="PKH54_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrgA protein"
                     /protein_id="HOC98532.1"
                     /translation="MKIDMNNRLVQLLQKRPGERFTARELAILFVENYPDDCEKKMEA
                     SSGLNNRDDLIAQITAEVGARRPRIQKRFPAVRITDERPRRYYYSESGESEQPVQGIA
                     PSVSDETSCDDVYQLGGRKFYEQDFYPLLSSYLWTELGVYSKRIDEKRSDHTRGPKGN
                     QWLFPDVVGMEFLADGWKRPVVDCATHHYDKLARLWSFEVKVRLRRGNVRQCFFQAVS
                     NSSWANLGYLVAATIDKDETDKELRILSAAFGIGVIRLNVENPSESEIIYPAQERPAI
                     EWGIVNRLVEANKDFEDYVEHVTQLYQTKKPHESDWDGPREEP"
     gene            complement(1143..4112)
                     /locus_tag="PKH54_01215"
     CDS             complement(1143..4112)
                     /locus_tag="PKH54_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOC98533.1"
                     /translation="MKLHFEPNLDFQRDAVDAVCDLFRGQERCRTEFTVTWKTDESLL
                     LQAGDNGLGIGNRLSILDDELLTNLRDVQFRNKLPPADKLESMDFTVEMETGTGKTYV
                     YLRTIFELNRRFGFTKFVIVVPSVAIKEGVYKTLQITDEHFAGLYDNPPRDCFVYDSA
                     KLGQVRDFATSPHIRIMVVTVGAINKKDVNNLYKESEKTGGERPIDLIRETRPILIVD
                     EPQSVDGGLEGRGKEALGAMNPLCTLRYSATHQQKFNMVYRLDAIDAYNRKLVKQIEV
                     ASATVEGGFNKPYIKLLATSNKGGRVTASVELDVQGAGGALRATKTVSDGDDLEQVTK
                     RAVYANHIIGEISADRTRPFMEVRIPGTEQWLTPGEAINDVDQDAVTRQLIRRTIREH
                     LDKERRLRPRGIKVLSLFFLGAVADYRLYDESGNAQKGPCALLFEEEYRKLMKHPDYR
                     DLFGGADVETEPELVHDGYFSIDRRGGKDFVKDTTGATKADDDTYALIMKDKERLLSL
                     DTRLKFIFSHSALREGWDNPNVFQICTLRDMKSEMQRRQTIGRGLRLCVAQDGQRVRD
                     TDTNVLTVVATGSYEEFAHDLQKEIERDTGFMFGHVGKDGFAAVGVRDGRAETPLGIQ
                     ASREIWQYLRNHGFVDGQGKVLDTLRTALKDKTFALPEEFAAQTAEIVGILTKAAGSL
                     EIRNADERVTVKTREKVLWGPDFKELWDRIRQKTMYRVAFDNERLISECTRAVAGMPQ
                     ITRTRITFQKAGLGIVTSGVICDRVAQYAPTTVEEGDIVYPDLLTDLQDRTQLTRRTI
                     VRILLDSGRLDDFRRNPQEFIDLAATAINKSKRQALVDGIKYSPLDGEVYAQELFETE
                     ELTAYLKNALPVEKSVYTHVIYDSAGVERSFAQDLEANDSVKVYAKLPPWFTVPTPLG
                     SYNPDWAVLVQTEEGDRLYFVVETKGSVFLDDLRGVEAGKIRCAREHFGAVDGSNAGC
                     RNDVRYVISANASDCLS"
     gene            complement(4281..6323)
                     /locus_tag="PKH54_01220"
     CDS             complement(4281..6323)
                     /locus_tag="PKH54_01220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOC98534.1"
                     /translation="MKKIDADDPMGKSADVAGNNFQRLRELFPEAFSEGHLDLQILRQ
                     LVGDLVVDEPTDASMPDEKYGLNWKGKRRARQLALTPSLGTLRPCPGESVDWDTTQNL
                     MIEGDNLEVLKLLQKSYSGKVKLIYIDPPYNTGKDFVYPDNFHDNIRNYLELTGQVDG
                     EGRRLTSNPESSGRFHTDWLNMMYPRLKLARNLLRDDGVLFMSLDDVEIGNARLLLDE
                     VFGPENLGAQIVWKKRSSPDARDSIGSVHDYLLCYIKNREFIKDALGKLPLSQDRVGA
                     YANPDNDPRGDWASVDMTGMTGRATKDQYFNIALPSGRIIRPPEGRSWGLAERTFIEL
                     RKDNRIWFGRDGNNIPRIKKFLGESDGQVIPSLWDSADSGSNDEGKKEINSLMGQPDV
                     FDTPKPVSLVQRMIRVGSTSRDEDIVLDFFAGSGTTGHATMKMNADDGRNRRYILVQL
                     PEVLDLSDKVQRSAADFCGTLCGPRTIAELTKERLRRASRKVREELLAKWVAEDAEAA
                     KRSNGPGLFESGEDKPAPERVMPDLGFRVFKLETSNIRVWNPDPSRLGEELLANAEHV
                     LPDRDDDDILYEMLLKLGLDLAAPMAERTIAGKKVVSIGNGALMACLEKEINASIVED
                     LAKGIAAWHAEQAPAGETTVFFRDSAFTDDVAKSNLTAILEQTFQGRKKDIRIRSI"
     gene            complement(6320..7123)
                     /locus_tag="PKH54_01225"
     CDS             complement(6320..7123)
                     /locus_tag="PKH54_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HOC98535.1"
                     /translation="MNSQTVVDALRIPDRLKVSTRVIGRTVFRQNAMLAPAELRALED
                     GLTELRVVATIRPSEFMIPAYTDDTREYLEILIMRGNLKSLAVADKIATVLHRAIPYP
                     LIVAFDLPAGGVYISLAHKRNPANKQGRMVVESANSVTISGKGGAAATESAFLASLDV
                     SKLPTRNLYVMYQAVWDRVTAMRAAASVGAFGVPADSDSAAELAASLVEFESISGEIL
                     ALQSRAERESQISRRVEINDEIGRLTPRYIAAAERMRAIMAHDGGKESV"
     gene            complement(7120..10353)
                     /locus_tag="PKH54_01230"
     CDS             complement(7120..10353)
                     /locus_tag="PKH54_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC98536.1"
                     /translation="MALIRNAGGDRVVDRLRKVLQDLESAGADVRPAIDIVTSDLSLF
                     AFDELRDLLNRIDHTRLLLSFDHDRVAGLLGVDGDRHYRNRLESRRIARQCLEWLNRR
                     ADVRDPVESLPQSTIVVRVLIMPAHVITGNCPFTTQGLGLTPGSRKGQIFEFSDADNL
                     NELSAWFADVWDEAAASSESARAALATQLQDLIAPKAPSLVYNIILQNIFAKTGGALV
                     EDEIIHHATGIRDTVVWKKLYRFQRDGVVGAVDKLERHGGCIIADSVGLGKTFEALAI
                     IKYYELRNDRVLVLCPKRLRDNWTVYRSNDVRNILAADRLNYDVLNHTDLSRGDGRSG
                     DIDLSRVNWGNYDLVVIDESHNFRNNPPHRDRVTRYERLMRDIIRQGVKTKVLMLTAT
                     PVNNRLSDLRNQISFITEGDEHALAPWGIDNIKITLDQAQAQFNKWLRLPDDQKSTDS
                     LVERLGCVYTQLLDLLTIARSRKHIVKYYGTNETGVFPERLKPVNFKPDVDLTSEFPP
                     VARINNEIRHLTLGAYSPLTYVFERKLPQYEKKYGTVLASGDRKFTQKDREQSLIHLM
                     RVNMLKRMESSVHSFGLTVSRQLQEVDAILERIDANPGEIEGLSFDDILDIDSPEFES
                     QLVGRKVKVLLQDIDLIKWRQDLQEDRNRLHTLKHLAERVTAERDAKLAQLRTLIAEK
                     AASPINPGNRKILVFTAFADTAEYLYRCLAPWARDELGMHVGLLTGAGSDKSTLPRLR
                     AEYNSILTAFSPRSKERPADLASEGEIDLLIATDCISEGQNLQDCDTLVNYDIHWNPV
                     RIIQRFGRIDRLGSINERVQLVNFWPNMEIEEYIGLERRVTGRMALLDVSATGEENLI
                     ESDSPEQMNDLEYRRKQLLKLQDAVPDLEDLSNGISITDSTLGDFRIDLAGFVRDNAG
                     LLDSQYPGTMAVTTVSSVDANDIPPGVIFCLRSVADISMTAVDTRYPLAPHYMVHVRD
                     DKTVVLDYMQTRRILDRLKKLCVGRDFPDLTAFERFDTATRNGRDMSAITAMLSAAVA
                     AVTGKHEERATASIFAPGGTLLQKGSFAGINDFEVVAYLVILPEAAQ"
     gene            complement(10525..11025)
                     /locus_tag="PKH54_01235"
     CDS             complement(10525..11025)
                     /locus_tag="PKH54_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98537.1"
                     /translation="MWRRLRYHFQPMFGASVFEEQSWGVGVMQPDGLNSPGWLIIDTF
                     IISPLQSIFEDPSHTVQSFGQILGLGLAVLVVVLIYTLPIALVLVLITRDPKDKSTLL
                     GQLLRDRRLRRSCDAYNQEMERYMQEHYPSDPDDANGVLTRRHEPDESEIVNGRIVNK
                     SNRNLK"
     gene            complement(11010..11378)
                     /locus_tag="PKH54_01240"
     CDS             complement(11010..11378)
                     /locus_tag="PKH54_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOC98538.1"
                     /translation="MLSPELIEKLLEFRRERDWEQFHTAKNLAAALSVEAGELLDIFR
                     WTRDPEIPATVASQRAAIAAELADVTIILSYLYHDLGIDPDQAVAEKLERNRAKYPVE
                     KARGTARKYREIRDGSCGEG"
     gene            complement(11448..12098)
                     /locus_tag="PKH54_01245"
     CDS             complement(11448..12098)
                     /locus_tag="PKH54_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98539.1"
                     /translation="MKSIIQVDEYKSTGEVTSTTQVGFLYCEKVDAGRPTCVPVEVQS
                     VPTNMTYSPLEMIRTGSGIYTSSSAPRERPAPESLTSYIPPVQQPPAAVPVARPAETT
                     PPPTKPLDTVEESAPPRTDSRATGNDRQPAATNAANEISVIYEFNSGWNSENAKSTLM
                     KWISRKVLVSKRDGKKLRGELLSIDVSTIIPGPGIVLWVDGERKSVLMKDILRITE"
     gene            complement(12421..12759)
                     /locus_tag="PKH54_01250"
     CDS             complement(12421..12759)
                     /locus_tag="PKH54_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOC98540.1"
                     /translation="MLSPEIIEKLLEFRRERDWEQFHTAKNLAAALSVEAGELLDIFR
                     WTRESEMQETVTAQRAAIAAELADVTIILSYLYHDLGIDPDEAVAEKLERNRAKYPVE
                     KARGTARKYR"
     gene            complement(12768..>13117)
                     /locus_tag="PKH54_01255"
     CDS             complement(12768..>13117)
                     /locus_tag="PKH54_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOC98541.1"
                     /translation="MLVTDAYHRRCAITGENTLEVLQAAHIRPYAMGGPHAITNGLLL
                     RMDFHKLFDDGLVSVSPDYRIHVSPRIRQKYYNGKVYYRLDDQPLTTMPDDLGLRPDR
                     ESLDWHFNNVFQR"
BASE COUNT         2660 a         3783 c         3662 g         3012 t
ORIGIN      
        1 agggatagat cctgtccatt atccaaaaac ctcccggtct gatttgttta acttcgcacc
       61 atcgactaaa ccatgcgatt tgttgctcga aacacttgcc gcgtaaagag cgactccctg
      121 tagaaagatt gctacggctc ctctcttggg ccatcccagt cggactcgtg gggcttcttg
      181 gtctggtaaa gctgagtcac atgctccacg taatcttcga agtctttatt agcctcgacc
      241 aggcggttaa caattcccca ctcaattgcg gggcgttcct gggcgggata aatgatttcg
      301 ctttcagacg ggttttcgac gttcaggcga atcaccccta tcccaaaggc agcacttaga
      361 atccgtagtt ctttgtcggt ctcgtcctta tcgattgtcg cagccacaag gtagcccaaa
      421 ttagcccagc tcgaatttga aacggcttgg aaaaagcatt gcctgacgtt cccccgccgc
      481 aaacggacct tgacctcaaa cgaccacaga cgtgcgagtt tgtcgtagtg atgtgtcgca
      541 cagtcaacaa cagggcgctt ccatccgtca gctaaaaact ccattcccac aacatcgggg
      601 aacagccact gattgccctt cggccccctg gtatgatcgg aacgcttttc gtcaatacgc
      661 ttcgagtaaa cgccgagttc cgtccacaag taggacgaca gcaaaggata gaagtcctgt
      721 tcgtagaact ttcttccccc aagctgatac acgtcgtcgc acgaagtctc gtcagaaacg
      781 cttggggcga ttccctgaac aggctgttcc gattcaccgc tctcggaata gtagtaacgg
      841 cgtggccttt cgtctgtgat ccgaacagcg ggaaaccgct tctgaatccg cgggcgcctg
      901 gcgccgactt ccgctgtaat ctgcgcaatc aaatcgtccc tgttgttcag cccggaactc
      961 gcctccatct ttttttcaca atcgtccgga tagttttcaa cgaacagtat ggccagttcc
     1021 ctggccgtga atctttcccc gggccgcttt tgtaaaagtt gaaccaacct gttattcatg
     1081 tcgattttca tgaaccattc cccataggct atgttccccg gtgccgaggg ccgggaaaat
     1141 gactagctca agcaatcaga tgcgtttgcc gagattacat aacgcacgtc atttctgcaa
     1201 ccggcattgg agccatccac cgccccaaaa tgttccctgg cgcaccgaat cttaccggcc
     1261 tcgacgccgc ggaggtcgtc caggaagacg gagcccttag tttccaccac aaagtaaagc
     1321 cggtcgcctt cttcggtttg gacgagtacg gcccagtcgg ggttgtagga gccgagtggg
     1381 gtcgggacag tgaaccacgg gggcagtttg gcgtatacct tcacggaatc gttggcttcg
     1441 aggtcctggg cgaaggagcg ttcgacgccg gcggagtcgt agatgacgtg ggtgtacacc
     1501 gacttttcga ccgggagggc attcttgagg tacgccgtca gttcttcggt ttcgaacagc
     1561 tcctgggcgt aaacctcgcc gtcgaggggg ctgtacttga tgccatcgac tagggcctga
     1621 cgtttggact tgttgatggc tgtggccgcg aggtcaatga attcctgggg attgcggcgg
     1681 aagtcgtcca gacgcccgct gtccagcaga atccgcacga tggtccggcg ggtcaactgg
     1741 gtccgatcct gcaggtcggt cagcaggtcg ggatagacaa tgtcgccctc ttcgaccgtt
     1801 gtcggcgcgt actgagcaac ccggtcgcag atgaccccgg acgtcacgat ccccagtccg
     1861 gccttctgga acgtgatgcg cgtccgggtg atctggggca ttccggcaac tgctcgggta
     1921 cattcactta tcagccgctc gttgtcgaac gccacgcggt acatcgtctt ctgacggatg
     1981 cggtcccaca gttccttgaa atccggtccc cacagaacct tttcgcgggt cttgaccgtc
     2041 acgcgttcgt cggcgttgcg gatttccagc gagccggccg ccttggtcag gatcccgacg
     2101 atctcggccg tttgcgccgc gaactcttca ggcaaggcga acgtcttgtc tttcagcgcg
     2161 gtacgcaggg tgtccagcac cttgccctgc ccgtccacga atccatggtt gcgcaggtac
     2221 tgccagattt cacggctggc ctggattccg agcggcgttt cagcacgccc gtcacgcaca
     2281 ccgaccgccg cgaagccatc cttgcccaca tggccgaaca tgaacccggt gtcgcgttca
     2341 atttctttct gaaggtcgtg ggcgaactcc tcatagctcc cggtcgcgac caccgtaagc
     2401 acgttggtgt cggtgtcacg cacccgctgg ccatcctggg caacgcacag ccgcagtccg
     2461 cggccgatgg tctggcgccg ctgcatttca gacttcatgt cccgcagggt gcagatctga
     2521 aagacgttcg ggttgtccca accctcgcga agggccgaat gcgaaaatat gaacttcaat
     2581 ctggtgtcca gggaaagcag gcgttccttg tccttcatga tcagggcgta cgtatcgtcg
     2641 tcggccttcg ttgccccggt tgtgtccttg acgaagtcct ttccgccgcg ccggtcaatc
     2701 gaaaagtacc cgtcgtgaac caattccggt tccgtttcga catcggcccc gccgaaaagg
     2761 tcgcggtaat cggggtgctt catcagcttg cgatattctt cttcgaaaag cagggcgcat
     2821 ggccccttct gtgcgttgcc cgattcgtcg tacaaacggt agtccgcgac cgcccccagg
     2881 aagaacaggc tcaacacctt gatcccacgc ggccgtagcc gcctttcctt atccaagtgc
     2941 tcccgaatcg tccggcgaat caactggcgc gttaccgcat cctggtcaac atcgttgatt
     3001 gcttcaccag gggtcaacca ctgttcggtc cccggaatgc gaacttccat gaatggtctc
     3061 gtccggtcgg cactgatttc gccgattatg tggttggcat acacggcccg tttcgtgacc
     3121 tgttccaggt cgtcgccgtc ggacaccgtc tttgtggcgc gcaacgcgcc gcccgccccc
     3181 tgcacatcca attcgacgct ggccgtcacc cggccgccct tgttagacgt ggccaacaac
     3241 ttgatgtaag gcttgttgaa gccgccctcg accgtggccg acgcgacctc gatctgtttg
     3301 accagcttgc ggttgtaagc atcgatcgcg tccagccggt ataccatgtt gaacttctgc
     3361 tggtgcgtcg cggaataacg aagggtgcaa aggggattca tcgcgcccag ggcttccttg
     3421 ccgcgtccct ccaggccgcc gtcaacgctt tgcggttcgt cgacaatcag gatcggccga
     3481 gtttcgcgga tcaaatcgat cggacgctcg ccgccggtct tttcgctttc cttgtacagg
     3541 ttgttgacgt cctttttgtt gatggcgcca acggtcacta ccatgatgcg aatgtgcggg
     3601 ctggtggcga aatcgcgcac ctggcccagc ttggccgagt catatacgaa acagtcacgc
     3661 ggagggttgt catacagccc ggcgaaatgc tcatcggtaa tctgcaacgt cttatacacg
     3721 ccttccttga tcgcgaccga cggcacgacg atgacgaact tcgtgaatcc aaaccgacgg
     3781 ttcagttcaa agatggtccg caggtacaca tacgtcttgc ccgtgcccgt ttccatttcg
     3841 acagtaaaat ccatggattc cagcttgtcg gcaggcggca gtttgttgcg gaactgaaca
     3901 tcgcgcagat tggtcagcag ttcgtcatcc aggattgaaa gccgatttcc gatcccgagc
     3961 ccgttgtcgc ccgcctggag taacaggctt tcatccgtct tccacgtcac ggtgaattcg
     4021 gtccggcagc gttcctgtcc ccggaacagg tcgcagaccg cgtcgacagc gtcccgctga
     4081 aaatccagat tcggttcaaa atgcagtttc atcatttgtc gagccttccc acgccccgca
     4141 cgaatcagtc atttccaaaa tggaaacaac tcagttgtcc ggaaaaaccg gacaactgaa
     4201 acagccacaa tttcaaacag ttccaactat ccggaatatc cggatggttg ctgaagccga
     4261 caatatcgtt cgtccatcat tcatatactc cgaatccgaa tgtccttctt gcggccttgg
     4321 aaggtctgtt caaggatggc ggtcaggttt gatttggcca cgtcgtcggt gaaggcgctg
     4381 tcccggaaga acaccgtggt ttcaccggcc ggagcctgtt cggcgtgcca ggcggcgatg
     4441 ccctttgcca agtcttcgac aatcgacgcg ttgatttcct tttccaggca ggccatcagg
     4501 gctccgttgc cgatggatac aaccttcttg cccgcgattg tgcgttcggc catcggggca
     4561 gccaggtcca ggccaagctt caacaacatt tcgtacaaga tgtcatcatc gtcgcggtcc
     4621 ggcagaacgt gttcggcatt ggcaagcagt tcttcgccga gcctggacgg atccgggttc
     4681 cagacacgga tgttgctggt ttcgagtttg aatactcgga agcctaggtc gggcatgacg
     4741 cgttcaggcg ccggtttgtc ctcacccgac tcaaacagtc cgggcccatt tgatcttttc
     4801 gcggcttcgg catcttccgc tacccacttg gcgagaagtt cttcacgaac cttcctactg
     4861 gcacgacgca gcctttcttt ggtgagttct gcgatcgtgc gggggccgca cagcgtgcca
     4921 caaaagtcag cggcgcttct ctgaactttg tccgatagat ctagtacctc cgggagttga
     4981 acgagaatat atcgccgatt ccttccatca tcggcattca ttttcatagt tgcgtgtccc
     5041 gttgtccctg acccagcaaa gaaatccaga acaatatcct catccctcga agttgaaccg
     5101 actcgaatca ttcgctgaac aagggagacg ggttttgggg tatcaaacac atcaggctgt
     5161 cccatgagtg aattgatttc cttcttgccc tcatcatttg aaccgctatc cgccgagtcc
     5221 catagcgacg gaattacctg accatcagat tcgcccaaga acttttttat tctcggaata
     5281 ttgttgccgt cacgtccaaa ccatattcga ttgtctttac gcaactcaat gaacgttctc
     5341 tccgcaagcc cccagcttct tccttctggc ggcctgatga tgcggccact gggtagcgct
     5401 atgttgaaat actgatcctt tgtggcacgt ccggtcatgc ctgtcatgtc aacagaggcc
     5461 caatcaccac ggggatcatt atctggattc gcataagcac ccacccgatc ttgggaaagt
     5521 ggcaacttcc cgagggcatc cttaatgaac tccctgttct ttatgtaaca aagcagatag
     5581 tcgtgaacgg agccaattga gtcacgggca tcaggtgaag aacgcttttt ccaaactatc
     5641 tgtgcgccca ggttctccgg accaaagact tcgtcaagaa gtaagcgtgc atttccaatt
     5701 tccacatcat cgaggctcat gaacaaaacc ccatcgtccc tcaaaagatt ccgggccagc
     5761 ttcaggcggg ggtacatcat gttcagccag tcggtgtgga agcggccgct ggattcgggg
     5821 ttgctggtca gtctgcggcc ctcgccatcc acctggccgg tcagttccag gtaattgcgg
     5881 atgttgtcgt ggaagttgtc ggggtaaaca aagtctttgc cggtgttgta tggcgggtcg
     5941 atgtatatca gcttgacctt gcctgaatag gatttctgta acagcttcaa gacttccagg
     6001 ttgtcgccct cgatcatcag gttctgggtc gtgtcccagt caacgctttc gccgggacat
     6061 ggacgcaggg ttcccaagga cggggtcagg gccaactgac gcgcgcgccg tttacccttc
     6121 cagttcaggc cgtacttttc gtcgggcata ctggcatcgg tgggttcatc gacaaccaga
     6181 tcgccgacga gctggcgaag tatctgcaag tccaggtggc cttcggaaaa cgcctcggga
     6241 aacagttccc gcagacgctg gaagttatta cccgcgacat ccgcgctttt gcccattgga
     6301 tcatccgcat cgatcttctt catactgatt ccttaccgcc atcgtgggcc attatggccc
     6361 gcatccgctc ggccgccgcg atataccgcg gcgtaagtct gccaatttcg tcgttgattt
     6421 caacccgtcg gctgatctgc gattcacgtt ccgccctgga ctgcagagct aagatttcgc
     6481 cggaaatgga ttcgaattcc acaagtgatg cagcaagttc ggccgcgctg tccgaatccg
     6541 ccggaacccc gaacgcaccg accgaggcgg ccgcacgcat cgcggtcacc cgatcccaca
     6601 ccgcctggta catgacgtaa aggttcctgg tcggaagttt cgacacgtcc aacgacgcaa
     6661 gaaacgccga ttcggtggcg gccgccccgc ccttccccga aatcgtgacc gaattcgccg
     6721 attcgaccac catccggccc tgtttgttgg cgggattccg tttgtgtgca agcgatatgt
     6781 acacgccgcc cgccggcaaa tcaaacgcca caatcagggg atacggaatg gcccggtgca
     6841 aaaccgtcgc gattttgtcg gcaaccgcca gcgacttcag attgccgcgc atgatcagaa
     6901 tttccaggta ttccctggta tcgtcggtgt aagcggggat catgaattcc gacgggcgga
     6961 tggtcgcaac cacccgcagt tcggtcaggc cgtcttccag ggcgcgcagt tcggccggcg
     7021 ccagcatcgc attctgccgg aacacggtgc ggccgatgac acgtgtcgat accttcagtc
     7081 tgtcgggaat tcgaagggcg tcgaccacgg tctgcgaatt cattgggcgg cctccggcag
     7141 aatgaccaga tacgccacca cctcgaaatc attgattccc gcgaatgagc ccttttgaag
     7201 cagtgtcccg cccggggcaa agatactggc agtcgcacgt tcttcgtgtt tgccagtgac
     7261 ggcggcgacc gcggccgaca gcattgcggt aatggccgac atgtcgcgtc cgttgcgcgt
     7321 cgccgtatca aaccgttcga atgcagtcag gtccgggaag tcccggccaa cacaaagctt
     7381 tttcagccgg tccaggatgc gccgggtctg catgtaatcc agtacgacag ttttgtcgtc
     7441 gcggacatgg accatgtaat ggggcgccag tggatatcgg gtatcgacgg cagtcatgct
     7501 gatgtccgcc acggaccgga ggcagaaaat cacgcctggc ggaatatcgt tggcatcgac
     7561 ggacgacacc gtggtcaccg ccatggtgcc agggtactgc gaatccagaa ggcccgcgtt
     7621 atcacgcaca aacccggcaa ggtcgatcct gaaatcgccc agggtcgaat cggtaatcga
     7681 aatcccgttg gaaaggtctt ccagatcggg cacggcgtcc tgcagtttca aaagctgttt
     7741 gcggcggtat tcaaggtcgt tcatctgttc cggcgagtcg ctttcgatca ggttttcttc
     7801 gccggtggcc gaaacgtcca gcagagccat cctgccggtc acgcgccgtt ccagcccgat
     7861 gtattcttcg atttccatgt tcggccagaa gttgaccagt tggacgcgtt cgttgatgga
     7921 cccgagccgg tcgatacgcc cgaaacgctg gatgatgcgc accggattcc agtggatgtc
     7981 gtaattcacc agggtgtcgc agtcctgaag gttctggccc tccgatatgc agtcggtcgc
     8041 gatcagcaga tcgatttcgc cctctgatgc caggtccgcg gggcgttcct tggaccgggg
     8101 cgaaaacgcg gtcaggatgg aattgtattc agcccgcagg cgcggcagcg tggacttgtc
     8161 ggaaccggcg ccggtcagca ggccgacatg catgcccagt tcatcgcgcg cccagggcgc
     8221 cagacaccgg taaaggtatt cggctgtatc agcgaacgcc gtgaatacca gaatttttcg
     8281 attccccggg ttaatcgggc ttgccgcctt ttcggcgatc agcgtgcgca actgggccag
     8341 cttggcgtcc ctttccgcag tgaccctttc ggccagatgc ttcagcgtgt gaagccggtt
     8401 ccggtcttcc tgcaggtcct ggcgccattt gatcaggtcg atgtcctgca acagcacctt
     8461 gaccttgcgc ccgacaagct gtgattcgaa ttccggcgaa tcgatgtcca ggatgtcatc
     8521 gaaagaaagc ccctcgattt cgcctgggtt tgcgtcgatc cgttcgagaa tggcgtcaac
     8581 ttcctgaagc tggcgcgaaa cggtcaggcc aaaagaatgg accgagcttt ccatgcgttt
     8641 cagcatgttc acgcgcataa ggtggatcag gctctgttcg cggtccttct gggtaaactt
     8701 gcggtcgccg ctggccagga ccgttccgta tttcttttca tactgcggca gtttgcgttc
     8761 aaagacgtac gtcagcggcg aatacgcgcc cagcgtcagg tgccggattt cgttgttgat
     8821 ccgggcgact ggcgggaatt cggaagtcag gtccacgtcg ggtttgaaat tgaccggttt
     8881 tagtcgttcg gggaagacgc cggtttcatt tgtgccgtaa tacttgacga tgtgtttgcg
     8941 ggaccgcgcg attgtcagca gatccagcag ttgggtgtaa acgcagccca ggcgctcgac
     9001 cagcgaatcg gtcgatttct ggtcgtcggg aagacgcagc cacttgttga actgggcctg
     9061 tgcctgatcc agcgtaatct taatgttgtc gatgccccat ggcgccagcg cgtgttcgtc
     9121 gccttcggtg atgaacgaaa tctggttgcg caggtcggaa agccggttgt ttacgggggt
     9181 cgcggtcaac atcagtacct tggttttgac gccctggcgg atgatgtcgc gcatcaggcg
     9241 ttcgtaacgg gtgacgcggt cccggtgcgg cgggttgttg cggaagttgt gcgattcgtc
     9301 gatcacgacc aggtcgtaat tgccccagtt cacgcgggac aagtcgatgt cgcccgaacg
     9361 cccgtcgccg cgggacagat cggtgtggtt caggacgtcg taattcagcc ggtcggccgc
     9421 caggatgtta cggacatcgt ttgaacgata aacggtccag ttgtcgcgca gacgtttggg
     9481 gcacaggacc agcacgcggt cgttgcgcag ttcgtaatac ttgatgatcg ccagggcctc
     9541 gaacgttttc ccgagcccca cgctgtcagc aataatgcaa ccgccgtggc gttccaattt
     9601 gtcaacggcg ccgacgacgc cgtcacgctg gaaccggtac agctttttcc acacaacggt
     9661 gtcgcgaatg ccggtcgcat gatggatgat ttcgtcttcg accagtgccc cgccggtttt
     9721 cgcgaagatg ttttgaagaa tgatgttgta taccagcgac ggggccttgg gggcgatcag
     9781 gtcctgaagt tgggtggcca gcgcggcgcg ggcggattcg gaacttgccg cggcttcatc
     9841 ccacacatcg gcgaaccagg cggatagttc attcaggttg tcggcgtccg aaaattcaaa
     9901 gatctggcct ttgcgactgc cgggggtcag gccaaggccc tgcgtggtga aggggcagtt
     9961 gccggtaata acgtgggcgg gcataatcaa aacacgaact acgatcgtgc tttgcggaag
    10021 cgattcgacg gggtcgcgga catccgcccg gcgattcaac cattcaagac actgacgggc
    10081 gattcgccgc gattcaagcc gattacggta atgccgatcg ccgtcgacgc ccagcagacc
    10141 ggcgacccgg tcgtggtcga acgacaacaa cagcctggtg tggtcaatac ggttcagcag
    10201 gtcacgcaat tcatcgaatg cgaaaagtga caaatcggag gtgacgatgt cgatagccgg
    10261 acgcacgtcg gccccagccg attcaaggtc ctgcagcacc ttgcgaagcc tgtcgacgac
    10321 cctgtcgccg cccgcatttc gaatcaaggc cattcccaca ccacgttaac cgactggcgc
    10381 aaatatatcc cgaaacaccc ctggtcgtca gcgacaaatc ggaccttgtc tgggggatac
    10441 cattggatca cagacaggcc gaagcaaagc gcctcaacgt aaatttgaca cagcatgcgg
    10501 caagatacgt gccgcgattt gaatctattt cagatttcga ttggatttat tcacgattcg
    10561 accgttgacg atttcggatt catcaggctc gtgtctacgt gttaaaacgc cgttggcatc
    10621 atcgggatcg cttgggtagt gttcttgcat gtatcgttcc atttcttgat tgtacgcatc
    10681 acacgaccgc ctcaatctcc tgtcacgcaa aagctgaccc agcaacgtgc tcttgtcttt
    10741 aggatcccta gtgatcaata cgagcacgag agcaattggt aatgtgtata tcaacacaac
    10801 taccaacaca gccagcccca gaccaagtat ctgaccaaag gattgcactg tgtgcgacgg
    10861 gtcttcgaaa atgctttgga gtggcgatat gatgaacgta tcaatgatca accacccagg
    10921 agaattcaag ccatccggtt gcataacgcc gacaccccac gattgttctt caaaaacaga
    10981 cgcaccgaac attggctgaa aatgatacct cagccttcgc cacatgaccc gtcacgaatc
    11041 tcgcgatact tcctcgccgt gcccctcgcc ttttccaccg gatacttggc cctgttccgt
    11101 tccagcttct ctgcaacggc ctggtcgggg tcgatgccaa ggtcgtggta gaggtaggac
    11161 aggatgatgg tgacgtcggc cagttcggcg gcgatggcgg cgcgctggga ggcgacggtg
    11221 gcggggattt cggggtcgcg ggtccagcgg aagatgtcca gcagttcgcc ggcctcgacg
    11281 gacagggcgg cggcgaggtt tttggcggtg tggaattgtt cccagtcacg ctcgcggcgg
    11341 aattccagca gtttttcaat aagttcgggt gacagcatcg ggggcctcag cgttgaaaga
    11401 cggtgttgaa gtgccggttg aacgcgcgta ctgaagccca aaaccgacta ttcggtaatc
    11461 ctaagtatat ccttcatgag cacgcttttt cgttccccat caacccaaag aacaatgccg
    11521 ggtccaggaa taattgtgga aacgtcaata cttaacaatt cgccgcgcaa ctttttgccg
    11581 tcccttttgc tcaccagcac cttgcgggaa atccatttca taagagtgga tttcgcgttt
    11641 tcggagttcc atccagaatt aaactcataa atcaccgata tttcattcgc ggcgttagtc
    11701 gcggctggtt gacggtcatt accggttgcc cttgaatctg tccgaggtgg ggcagattct
    11761 tcgacggtat ccaatggttt agtcggcggc ggtgtcgttt ccgcaggcct ggccactggc
    11821 accgccgcag gaggctgctg gacaggggga atgtacgacg ttagagactc tggcgcggga
    11881 cgttcccgcg gcgccgaact agaagtgtaa atgccacttc cagttcgaat catttcaagc
    11941 ggcgaatacg tcatgttggt cggcaccgac tgcacctcga ccggcacaca agttggccgc
    12001 ccggcgtcaa ccttctcgca gtaaaggaat ccgacctgag tggtcgacgt gacctccccg
    12061 gtgcttttgt actcgtcaac ctggattatg cttttcatcc acaatggact tccagtcccc
    12121 aggctttggt gttccgtccg gatttttcga tatgagtacg tatttgaatt cgttaccgtc
    12181 gagcatgcgg caagactggc gacaaccaag aaagccgcag gggtcgcaaa cgacaccttg
    12241 gaattaagca ttgaccaagt cacggcacac ctcgaaatag gctaatttcg ccttattgga
    12301 tatcactgcg ctttgacaaa atcaattaga ttgagatgga gaactcaggc cccgagagtt
    12361 tcaagcaggc cggatttggc cggaccgatt caagcgatca taaatcaaac agcccgacat
    12421 ctatctgtac ttcctagccg tccccctcgc cttctccacc ggatacttgg ccctgttcct
    12481 ttccaacttt tcggcaaccg cctcatccgg atctatgccc aggtcgtggt acagatacga
    12541 caggatgatg gtgacgtcgg ccagttcggc ggcgatggcg gcgcgctggg cggtgacggt
    12601 ttcctgcatt tcggattccc gggtccagcg gaagatgtcc agcagttcgc cggcttcgac
    12661 ggacagggcg gcggcgaggt ttttggcggt gtggaattgt tcccagtcgc gctcgcggcg
    12721 gaattccagc agtttttcaa tgatttcggg tgacagcatc gggggcctca gcgttgaaag
    12781 acgttgttga agtgccagtc caggctttcc cggtcggggc gcaggccaag gtcgtcgggc
    12841 atggtggtca ggggctggtc gtccaagcgg taatacacct tgccgttgta gtacttctgg
    12901 cggatgcggg ggctgacgtg aatcctgtaa tccggcgaca cgctgaccag gccgtcgtca
    12961 aacagcttgt ggaagtccat gcgcagcagc aggccgttcg tgatggcgtg gggaccgccc
    13021 attgcgtacg gccggatgtg ggccgcctgc aggacctcca gagtgttttc gccggtgatg
    13081 gcgcagcgcc ggtggtacgc gtcggtcacc agcatct
//
LOCUS       DAOPPA010000019        13083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5542_length_13083_cov_4.401597, whole genome shotgun sequence.
ACCESSION   DAOPPA010000019 DAOPPA010000000
VERSION     DAOPPA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5542_length_13083_cov_4.401597"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..10809)
                     /locus_tag="PKH54_01260"
     CDS             complement(<1..10809)
                     /locus_tag="PKH54_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98542.1"
                     /translation="MSTSSLDKMFEIIFSDTPDFSVARECGADMSGAELIETLSLIRE
                     KALEASGPEMVHSLAAVVANLLLGAALDNPFDAEADKTARNSVSRIAGAAADLFDQDV
                     GDQSLAQVARLAEAILSLNPAALADVYDLFGQGLDEDVKIRLLRLLAGCGDADYNRRY
                     GPTLGEALAARRARMAAEATPADGVEQVMQEDVFPEDVGISEDAGISEDAGISEDAGI
                     SEDAGISPEKNVDAGDDSSPDLDDPAQRQQTLQFEPEDREADGIASSEPAEDADSGDA
                     GSSDADVSVPADLAGLEAGLNRAPKDRDLRGAYIRLAIEQEVAERAVKFISRIAAGLH
                     DADKAAALVDLAALHLMGGGTEAAMTALEAALEADRTSREALDRYVAIAEEGGLSERG
                     AAFLETVRRALTGTPSEIPLLTSLGRLLGLSGRDDDAEKLWRRLRALDPRNIEALEFY
                     IGYYGRKSDWQKQFATAQFELSVVDSDRDRIRLNLLMADVAENRLNNLDRAAEAHKRI
                     LSLAPENLDSFEALVCLYEKSRKWHQLVEMYNDRIRRLPAEDVDQKVALLFRIVEVYQ
                     NPEKQPGQENMLAAYSRIADISPTNVQALDTLDRGYQTSERWPDLLKVLEKKIELTED
                     PVELLELFNRVAEIVINRMSNETQAIPFLEKILELDPENLDVVLKLKSIYSHKHNQEK
                     HYAMLKREIAMVQGAEREKILLSAAEMARDKLLLFDEALELYSEAYRQNGTLREAREN
                     MHALFDRMERWADYAVFLDSEVECEMPDRRRIELLHKLAEIRATHLGDPVGAETVFQK
                     ILSIDPSDDLATDRLEAMLLQQGRFQEIYDVYRKMDDVRKFVAQITSFDAQKGVNPGF
                     RKEMCLILAQACEVDLKEPERALEFMEQAFQADPADDSVGRKVLSTAMKKKDFKRAER
                     VLKILTATASEPAARQEDFVKLSKLYRDTERPSDAFDALADGIRADLDSDGLQKLVAE
                     AIDSGTAGELWQPLATLLSDVVTAPSDDKWRQQILMVLGRIQAERLLFHEDARTTFEK
                     VLAIDPGALEALDVLESIAMQQQDFTALEGVLIRRIDALKLDSDRQAVLVRLGALYED
                     LIGDDDKAAAAFARAAELAAEPDRAVLAGLHRTYDRLERFPELADVIRKERLLAPQDW
                     ERTALDIELAAVLADGLGQFDDALAVLDGILCDADNDKALALVRRIFDDGSDTDTAGS
                     ILRTVYARTGDSAGLLGVIETMIERAGRNDAKAALLVQAAGILSADLGDPSGAFERLA
                     SSIRLFPTEENVTLALGLAQEVDGFRLLADAIQSVVDPDVAAAEDLPELDPEIQALLF
                     GTLGNLYGRKLGQPGKAVMAMERVMALTDVDESFLAEMLTLYRAVDDVDSALSAFDRL
                     QAAQSVADARSTLIEKAMYAAANGRIDTAVAALRDVAGRTFDPEADSLLEEMLLEHGR
                     YGDLIELLQARTERPEYESERAETAFRIAGVFRTHMKMASEAARFVRQAIKESQGRAD
                     IVAFAVEMVLDRSISDRRSFAPELAETLIDTLEAGQGDSGTVADLLRLRAEFCDDSIQ
                     RVAILNRLAALQDSTGDHVGRFDTLSQALGNTPGDAELAEAFVAAAVKAGAAVRAVER
                     LGRFASSASGSDRVRLLMAAAALAKDHAGDEPGAVLIYRGLLNEFPDDVQVIEALEQL
                     LQAMGSDAERVPLLERLAVLADDLSRRRTIRLKAAIIAASSNDPLTASGFLKFVVDAR
                     PDEDALDAESEDAARRLVDVSMELDDFDTVAEMHLLLGRLLPDAEARRACLLAAAEVT
                     RDRLGHPADALTIFTGLLKVDENDAVARDAARDLARKTENVRVLMDILRHDVATAVDS
                     SARNAALLESATVSLRIDVNDTDGAIEALRLVLLDDPSNVAAVRLCESIYDDHEAAPE
                     AARLLIDAAERSGDGELLVRTLRIAIGQTGDPVERAAKLKTVGTTLVKLDRNAEAVEA
                     FADAFLERPSEPETLRDLFDQLVRLERPELLSERVDAACDLMPDGAIDDVIDLRLRAA
                     ALLNTIPGKAEDAFVILRRALALRESDPAVIRAIIDLTQEAGMWRHYVEIQRLNIAYL
                     LSTPDEKADAWLECGAIYADADRLDDQDLAAECYREVLAIRPLDRAALDVLARILGAA
                     GDTEGLRILWQCELAAWEGLSPEQRDEGRMTRLRISVAEGALASGGGSDAVSAAMAII
                     ESKAADAHSLDVAVKIYRETLDLELFNQLTALLSERNEHRRLLDLYRFVYSRKDVPPG
                     PEVCLRKSIELDEKLRLHDFRFEDLGRLIELVPTDAANMATYVEVGRKLGRMVKVAAL
                     LEKTLAAFQDREVAFEVAMQLAEIYEVDLKKPEDATNYLRLAFLRRPEDRLVVERLAA
                     AYIGLGQFGDLALLYENLGDLTVDASERIEHYFKAAEVQREKVASPDGAIEILKKIMD
                     IDPSNMAAIDALEQVSRQTGNYPELAVWLQHRADVTTDSEARRTLLLELADLYSTRLG
                     RPGDAASVLESLQEALPQDLEVWARLEKTLLSLGTFDRLADMYMRLAGVVEADEHRLA
                     ALKKAASVFEARLDDRFQAVHALQEILKIDPRDSFACVRLGEMLEADEDWPALARHLA
                     GCCNASEDAQARAGLALRVADLYMSRLDRQADAIAYFKMALDFDPKIAEAREGLMSLV
                     GEGECSVDAAVILEEVFSRTGESASLLEVLRRQLGTVHTGSERAAILMRMAEVQAALP
                     GMAGEAAALVGQALAEAPGKVDVLARLQAMAEGAGLWQQAYDLVAAALANAADDEALA
                     RLHRFAGTVAGGKLDDFERAAAHLETFLALQDVDAEVLEMLDMIYGRLGRRADQADIL
                     RQRISLAGDDVDSALQLRLAAALMDDSRDVDTAFDIARRVLKREPSNQDALKFMTDIT
                     SDPIVGRRAMDVLRRAFRESRNDSELLRAVENQILNSHESAELIELHCEAAGAAGSLG
                     NSELQLVHLGAALALDPSSDKVLADVLETAGRNGNGKLAWAILKRAAQAASWPDLEKK
                     LLLDAVALAGRTGFDDQGQTEAALRRVLEIDPANRTALDALDATFKDAGRQTDLMAML
                     RHKLRLNLSQEERVSTLLRLGGILEGSGELKQAAESFEEAVVLEPGDLNLLNRLRAIY
                     ANLGDVKGQIDTIERVSIAARTPKARISGLLEVAALQKDQLGDLKAAMATLERVIADD
                     PGNLAGRQRLEEVYEELGDFQALVRLLSNVLDSKAEAAEKVRCAMKAAQISEIELDNV
                     PMAISLVRRAWSVEPENREVIDDLMRLYFKTEDWTSLIGVVRARAAIETNEAARMQAL
                     VTAVQIAVEQVGDQGLAAIISREILQIRPDHHESILLLAEMAESRQQDSEALELFKQL
                     AEPDVPAKFRIDGLLGTARLLVRAGGEGDVAAEYIRQAAALDPEHPAVRKFLKASYLE
                     DGEYSKLAECLDLELAAAGTDEERAAVSMDMAEIYLNHLSDGEKFLEYALKAYGYRRD
                     DPRTVTALVDFFLKNGRDSEAVPYLEWLVNYLEAKRLLKELPPYANALGRILEASGDG
                     RRAVDFYRICHEHDAANIDNALALGRLHIRHNDMEKALRVMQPMLLRIDSLPLDQKKE
                     ILLSLARINENRGDLKKARQF"
     gene            11054..>13083
                     /gene="dnaE"
                     /locus_tag="PKH54_01265"
     CDS             11054..>13083
                     /gene="dnaE"
                     /locus_tag="PKH54_01265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOC98543.1"
                     /translation="MSFVHLHLHSHYTLLNSTIRITDLVDKAVSSGVPAVALTDTMNV
                     FGSLELEKKIRGKGVKPIYGAELLISTDPGVAPFSLVVLCRNREGFANLREILSRAAT
                     ESPRDDCHCTTRDILASLSKGLIGLSGSLGGQIPHAILTGGQAEQLASEYASIFEPGC
                     FFLELQGNDLEEQKTVNRELAAIGQRIGLPCVATNNVHYLERSDAPAHAILVAIGQRK
                     RLSFESLDNFPLDSFHFASEADMRRAFCWIPEAVDNTVKIAEMIEPDILFSSQKHFFP
                     VYQPPEGMTVGEYLTKLAKEGLADRLANSRKTGFEKDPAEYWARLDRELEVIIGQHFD
                     TYYLVVWDFINWSRQNDVPVGPGRGSGAGSLVAYSIGITDVDPLSYDLLFERFLNSER
                     ISPPDFDIDFCKDERSRTIEYVTNRYGREQVCQIVTFGTLKARAAIKDAARVLGFSFD
                     ESDVLTKMIPEEPGMTLARAAEIDSRIEQVCKSDARFGRLWEVALKLEGLARQTGKHA
                     AGVVIADRPVKEYAPLYVNDDGSIVTQYDMANLDTVGLIKFDFLGLLSLTIIAHALKN
                     IRARKDPGFDLGRIPLDDRATFDLLCSGRTAGVFQLETRGITNQVKRLRPDTLEDIVA
                     TIALFRPGPLQNKMDEEFIAVKHGQKKAQYAHPLLEPILKDTYGTILYQEQV"
BASE COUNT         2653 a         4246 c         3671 g         2513 t
ORIGIN      
        1 gaactgcctg gccttcttaa ggtcgccgcg gttttcgttg atgcgggcaa gggaaagcag
       61 gatctctttc ttctgatcca gcggcagtga atcaatgcga agcagcatcg gctgcatcac
      121 gcgaagcgcc ttctccatgt cgttatgacg gatgtggagc ctgcccagcg caagggcgtt
      181 gtcgatgttc gcggcatcat gttcgtgaca gatccgatag aaatcgacgg ccctgcgccc
      241 gtcgccggac gcttcaagaa tgcggccaag cgcgttggcg taaggcggaa gttccttcag
      301 aaggcgcttt gcttccaggt agttcaccaa ccattccagg taaggaaccg cctcggaatc
      361 gcggccgttc ttcaggaaga aatcgaccag cgccgtcacg gttcgcgggt cgtcgcgccg
      421 ataaccgtaa gccttcagcg cgtactccag gaacttctcg ccatcggaca gatggttcag
      481 gtaaatctcg gccatgtcca tgcttacggc agcgcgctcc tcgtcggttc cggccgcggc
      541 cagttcaaga tcaagacatt ctgccagttt ggagtactcg ccatcttcca ggtatgacgc
      601 cttcaggaac ttgcgcaccg ccgggtgctc gggatcaagg gcggccgcct ggcgtatgta
      661 ctcggccgcg acatcgcctt ccccgccggc gcgaacaagc agcctcgccg ttccaagcag
      721 tccgtcgatc ctgaacttcg ccggcacatc cggttccgca agctgcttga acagttcaag
      781 ggcctctgaa tcctgctggc gggactcggc catttccgca agcagcagga tcgattcgtg
      841 gtgatccggc cgaatctgca gaatttcccg cgaaatgatg gctgcaagcc cctggtcgcc
      901 gacctgctcg accgcaatct gaaccgcggt gaccagagcc tgcatcctcg cggcctcgtt
      961 ggtttcgata gccgcacggg ctcgaaccac gccgatcagt gacgtccagt cttcagtctt
     1021 gaagtaaaga cgcatcaaat cgtcaatcac ttcacgattt tccggctcga cagaccacgc
     1081 ccttctaacc agcgaaatgg ccatcggcac gttgtccagt tcgatttcgg aaatctgggc
     1141 cgccttcatc gcgcaccgaa ccttttcggc tgcttcggcc ttcgaatcaa gaacgttcga
     1201 cagaagccgg acaagggcct ggaagtcacc gagttcctca tagacttctt ccaggcgctg
     1261 cctgccggcc agattgccgg gatcgtccgc gataacgcgt tccagggtcg ccatcgccgc
     1321 cttcaggtcg ccaagttgat ccttctgaag cgccgcaacc tccagcagac cggaaatcct
     1381 ggccttgggg gtcctggccg cgatcgacac gcgctcgata gtgtctatct ggcccttcac
     1441 gtcgccaaga ttggcatata tggcccgcag cctgttcaac aggttcaagt cgccaggttc
     1501 caggacgacc gcttcctcga acgactcggc cgcctgcttc aactcgcccg agccttcaag
     1561 gattccacca agacgcagca gggttgaaac cctctcttcc tgggacaggt tcaagcgcag
     1621 tttgtgacgc agcatggcca tcaggtcagt ctgccgacct gcatccttga aggtcgcgtc
     1681 cagagcgtca agcgccgtcc tgttggccgg atcaatttca agcacgcggc gcaacgccgc
     1741 ctcggtctgg ccctgatcat caaaaccggt ccggccggcc agcgcaacgg cgtcaagcag
     1801 caacttcttt tccagatcag gccatgatgc ggcctgggcc gcacgcttca atatggccca
     1861 cgccagcttg ccgtttccat tccttccggc cgtctcgagc acgtcggcaa gcaccttgtc
     1921 gctggacgga tccagggcca gtgccgcgcc aagatgcaca agctgcaatt cgctgttgcc
     1981 cagggagccg gccgccccgg cggcctcgca atgaagctcg atgagttccg ctgattcgtg
     2041 tgaattcaga atctgatttt caaccgcgcg caacagctcg gaatcgttac gagactcgcg
     2101 gaatgcccga cgaagcacgt ccatcgccct gcgcccgacg ataggatcgc tggtgatgtc
     2161 cgtcatgaac ttcagcgcat cctggttcga tggctcgcgc ttcagaaccc tgcgggcaat
     2221 atcaaaagca gtatcaacgt ccctggaatc atccatcaat gccgcggcca gccgaagctg
     2281 cagagccgaa tcaacatcgt ctcccgcaag cgatatacgc tgcctcaaaa tatctgcctg
     2341 gtcggcacgg cgcccaagac gaccgtagat catgtccagc atttcaagga cttccgcgtc
     2401 cacatcctga agcgccagga acgtctccaa atgcgccgcg gcccgctcga agtcatccag
     2461 cttcccaccg gccaccgttc ccgcgaaccg gtgcagccgg gccagcgcct cgtcgtcggc
     2521 cgcgttcgcc agggcagccg cgaccaggtc gtaagcctgc tgccagagcc ccgcgccttc
     2581 cgccatcgcc tgcagcctgg caagcacatc gacctttcca ggtgcctcgg caagcgcctg
     2641 accgacaagc gcggcggctt cgccggccat tccaggcagg gctgcctgaa cctcggccat
     2701 ccgcatcaga attgccgccc gctcgctccc ggtatgcacg gttccaagct ggcgcctgag
     2761 gacttccagc agactcgcgc tttccccggt tcttgaaaag acctcttcca gaatcaccgc
     2821 cgcatcgacg gagcactcgc cctcgccgac aagcgacata agcccctcgc gggcctcggc
     2881 aatcttcgga tcgaagtcca gcgccatctt gaagtacgcg atggcgtcgg cctggcgatc
     2941 aagcctggac atgtaaaggt cggcgacccg cagggccagt ccggcccgcg cctgggcgtc
     3001 ctcggatgcg ttgcagcacc cggccaggtg tcttgcaagt gccggccagt cctcatcggc
     3061 ttcgagcatc tcgccaagac ggacgcacgc gaagctgtcc cgcgggtcga tcttcaggat
     3121 ctcctgcaag gcatgaacgg cctggaagcg atcatccagg cgtgcctcga acaccgaggc
     3181 cgccttcttc aacgcggcaa gacgatgctc atcggcctcg acgacgcccg ccagccgcat
     3241 gtacatgtcg gccagcctgt cgaaggttcc aagagacaga agagtctttt ccagccgcgc
     3301 ccagacttcc agatcctggg gaagcgcttc ctggagactc tcaagcaccg aggctgcatc
     3361 accgggacgc cccaaccttg tggaataaag atccgcgagt tccagcaaca gggttcgcct
     3421 tgcctcggaa tccgttgtca cgtcggcccg atgctgcagc cagaccgcca gttcaggata
     3481 gttgccggtc tgacgggaga cctgctcgag cgcatcgatt gccgccatgt tcgacggatc
     3541 gatatccatg attttcttca gaatttcgat ggcgccatcg ggacttgcaa ccttttcgcg
     3601 ctggacctcg gccgccttga agtaatgctc gatgcgctcg gacgcgtcaa cggtcaagtc
     3661 accaagattt tcgtaaagca gcgccagatc gccgaactga ccaagcccga tgtaagccgc
     3721 ggcaagcctt tcaaccacca ggcgatcttc cggccgccga aggaaagcaa gccgaaggta
     3781 atttgtggcg tcttccggct tcttcaggtc aacttcataa atttcagcaa gttgcattgc
     3841 aacttcgaaa gcaacttcac gatcctggaa ggcggccagc gtcttttcaa gcagtgccgc
     3901 caccttgacc atccgcccaa gtttccgccc cacctcgaca taggtcgcca tgttcgccgc
     3961 atcggtcggc accagttcga tcagacggcc gagatcctcg aacctgaaat cgtgaagccg
     4021 cagtttctcg tcaagctcaa tcgacttccg caggcagact tccggccccg gcggaacatc
     4081 ctttctggaa tagacgaagc ggtaaagatc cagcaggcgg cgatgctcgt tgcgttcgga
     4141 aagcaacgcg gtgagctggt tgaacagttc aagatccagg gtttcccgat aaatcttgac
     4201 cgcaacgtca agcgaatggg cgtccgcggc ctttgactcg atgattgcca ttgccgccga
     4261 aaccgcgtcg gatccgccgc ccgacgccag cgcgccttcg gccacgctga tccgcagcct
     4321 ggtcatgcgc ccctcgtccc gctgctccgg ggaaagccct tcccacgcgg caagctcgca
     4381 ctgccacaga atccgaagcc cctcggtgtc gccggccgcg ccaaggattc gggccaggac
     4441 gtccagagcc gccctgtcca gcggcctgat ggccaggact tcacggtagc actcggcagc
     4501 cagatcctga tcgtcaagac ggtcggcatc ggcgtaaatc gcgccgcact caagccaggc
     4561 atcggccttc tcgtccggcg tcgaaagcag ataggcgatg ttcaggcgct ggatctcgac
     4621 gtaatgccgc cacattccgg cttcctgcgt cagatcgatt atcgcccgga taacggccgg
     4681 gtcgctttca cgcaaggcca gagcccggcg caatatcacg aaggcatcct cggccttgcc
     4741 cggtatcgtg ttcagaagcg cggcggcccg cagccgaaga tcgattacgt cgtcaatcgc
     4801 gccgtccggc atcaggtcac acgccgcatc gaccctttcc gaaagaagtt cgggtctttc
     4861 cagcctgaca agctgatcga acagatcccg cagggtttcc ggttccgacg gcctttcaag
     4921 gaaggcgtcc gcgaaggcct caaccgcctc ggcgttacga tccagtttga caagggttgt
     4981 cccgacggtc ttcagcttcg cggccctctc gaccggatcg ccggtctgtc cgatcgcgat
     5041 tctcaacgtc cgaaccagca gttcgccgtc ccctgaacgc tccgcggcat ctatcagcag
     5101 acgcgcggcc tccggggcgg cttcatggtc gtcatagatc gattcgcaca gcctgaccgc
     5161 cgcaacgttc gacggatcgt caagaagaac caggcgcagc gcctctatcg cgccatcggt
     5221 atcgttgaca tcgatccgca gcgatacggt ggcggattcc agcagggccg cgtttcgagc
     5281 gctcgaatca acggcagtcg ccacgtcgtg acgcaggatg tccatcagaa cccggacatt
     5341 ttcggtcttg cgggcaaggt cgcgcgccgc atcccttgcc acggcatcat tctcatcaac
     5401 cttcagcaaa ccggtgaaga tagtcagggc atcggccggg tggccaagcc gatcccgggt
     5461 gacttcggcg gcggccagca ggcaggcccg gcgcgcctcg gcatccggaa gcagccgacc
     5521 aagaagcagg tgcatttcag ccaccgtgtc gaaatcgtcc agttccatcg aaacatcgac
     5581 aaggcgcctg gccgcatcct cggactcggc atcaagcgca tcctcatccg gtcttgcatc
     5641 gacgacgaac ttcaggaacc ccgaagccgt caggggatca ttggaggacg ccgcgataat
     5701 ggcggccttc aggcgaatcg ttcttcgccg ggaaaggtcg tccgccagga cggccaggcg
     5761 ttccagcaac ggaacccgtt cggcatcgct tcccattgcc tgaagcaact gttcaagcgc
     5821 ctcgatgacc tggacgtcat ccggaaactc attcagcagc ccgcgataaa tcaggacagc
     5881 gccaggctcg tcgccggcgt gatcctttgc cagcgcggcg gcggccatca gaaggcgaac
     5941 gcgatcggaa ccggatgcgg atgacgcaaa acgtccaaga cgctcgaccg cgcgaacggc
     6001 ggcgccggcc ttgacggcgg cggcaacgaa ggcctcggca agttcggcat cacctggcgt
     6061 gttgccaagc gcctgggaaa gcgtgtcgaa tcggccaacg tggtcaccgg tcgaatcctg
     6121 aagcgcggcc aatctgttca ggattgcaac acgctggatt gaatcgtcac agaactctgc
     6181 ccggaggcga agcagatccg cgacggtgcc ggaatcaccc tgccctgcct caagggtatc
     6241 tatcaaggtc tcggccagtt ccggcgcgaa agacctgcga tccgaaatcg atcgatcaag
     6301 caccatttcc acggcaaagg cgacgatgtc ggcgcggccc tgggactcct ttatggcctg
     6361 cctcacgaac cgggctgcct cggaggccat cttcatatgg gtcctgaaga cgcccgcaat
     6421 cctgaaggcc gtctcggccc tctctgactc atattccgga cgttcggtcc tcgcctgcaa
     6481 cagctcgatc aggtcgccgt acctgccatg ttcaagaagc atttcctcca gaaggctgtc
     6541 ggcttccgga tcgaaggtgc gacccgccac gtcccgaaga gccgcgaccg cggtatcgat
     6601 ccggccattg gcggcggcgt acatcgcctt ttcaatcaga gtgctcctgg catcggcgac
     6661 cgattgagca gcctgaagcc tgtcaaaagc cgaaagcgcc gaatcgacat catcgaccgc
     6721 gcggtaaagc gtcaacatct cggccaggaa ggactcatcg acgtcggtca gggccatgac
     6781 gcgttccatc gccatgaccg ccttcccggg ctgccccagt ttcctgccgt aaaggtttcc
     6841 aagcgtccca aaaagaagcg cctggatttc gggatccagt tcaggaaggt cttccgcggc
     6901 cgccacatcg ggatcaacga cggactggat ggcatccgca agcagcctga atccatcgac
     6961 ttcctgtgca aggcccaggg ccagcgtgac attctcctcg gtcgggaaca gcctgatcga
     7021 tgaagcaaga cgctcgaagg cgccggacgg atccccgagg tcagccgaaa ggattccggc
     7081 cgcctgaaca agcagggccg cctttgcatc gttacggccg gctctctcga tcatcgtctc
     7141 gatcacgccg agcagaccgg cggaatcccc ggttcttgca tagacggttc gaaggatgct
     7201 tccagcggtg tcggtatccg acccatcgtc aaaaatccgc ctgaccagcg ccaacgcctt
     7261 gtcgttgtcg gcgtcgcaga gaattccgtc aaggacggcc agggcatcgt cgaactgccc
     7321 gagcccgtcg gcaagcacgg ccgcgagttc gatatccagg gcggtccgct cccagtcctg
     7381 gggcgccagc agcctttcct tcctgatgac gtcggcgagt tcggggaacc tctcgagcct
     7441 gtcgtaggta cgatgaagcc cggccagcac ggccctgtca ggctcggcag ccagttccgc
     7501 cgcccttgcg aaggctgcgg cggccttatc gtcatcaccg atcaggtcct cgtaaagggc
     7561 gccaagcctg acaagaacgg cctgcctgtc ggaatcgagc ttcaaggcat cgatgcgccg
     7621 gatgaggaca ccttcaagag ccgtgaagtc ctgctgctgc atcgcgatgg attcaagaac
     7681 atccagggcc tccagcgcac cgggatcaat ggcgagaacc ttttcaaaag ttgtcctggc
     7741 gtcctcgtga aaaagaaggc gctcggcctg gatgcggccc agaaccatca ggatctgctg
     7801 acgccactta tcatcggagg gggctgtcac gacgtccgaa agcagcgtgg cgagcggctg
     7861 ccacaattcg ccagcggttc ccgaatcgat ggcttccgcg accagtttct gaagcccgtc
     7921 cgagtccaga tccgccctga tgccgtccgc aagcgcgtcg aatgcatcgg aaggcctttc
     7981 ggtatcgcga taaagcttcg aaagtttaac gaaatcttcc tgccgcgccg ccggttccga
     8041 tgcggtggcc gtcagaatct tcaagacgcg ctcggcgcgc ttgaagtcct tcttcttcat
     8101 cgcggtggaa agaaccttgc ggccgacgga atcgtccgcc ggatcggcct ggaaagcctg
     8161 ctccatgaac tccagcgcac gctcgggctc cttcaggtcg acttcgcagg cttgggccag
     8221 tataaggcac atctccttgc ggaacccggg attcaccccc ttctgcgcgt cgaatgaagt
     8281 aatctgggca acgaacttcc tgacgtcgtc catcttccga taaacgtcgt agatctcctg
     8341 gaagcgcccc tgctgaagaa gcatcgcctc caggcggtcg gtcgcaaggt cgtcgctggg
     8401 atcaatcgaa aggatcttct ggaaaacagt ttccgcgccg acaggatcgc caaggtgcgt
     8461 cgcgcggatt tcggccagct tgtgcagaag ctcaatccga cgccggtcag gcatctcgca
     8521 ctcgacttcc gaatccagga aaacggcgta atcggcccat cgctccatcc ggtcgaacag
     8581 cgcgtgcatg ttttcgcgcg cctcccgaag ggtcccgttc tgccggtatg cctcgctgta
     8641 caactcaagc gcttcatcga aaagaagcag cttgtcgcgc gccatctcgg ccgccgaaag
     8701 caggatcttt tcccgctcgg cgccctgaac catcgcgatt tccctcttca gcatcgcata
     8761 gtgcttttcc tggttgtgct tatgactgta aatgctctta agcttcagga cgacatccag
     8821 gttctcgggg tcgagttcca ggatcttttc caggaaaggt atcgcctggg tttcgttcga
     8881 catcctgttg ataacgattt cggcgacacg attgaacagt tcaagaagtt cgaccggatc
     8941 ctcggtcagc tcaatcttct tttcgagaac cttcaacaga tccggccacc gctcggacgt
     9001 ctggtaaccg cgatcgagcg tatccagcgc ctggacattg gttggggaaa tgtcggcgat
     9061 acggctgtaa gccgccagca tgttttcctg gcccggctgc ttttccgggt tctgatagac
     9121 ctcgacaatc cgaaacagca aggcgacctt ctgatcaacg tcctctgccg gaagccgccg
     9181 aatacggtcg ttatacatct cgacaagctg gtgccatttc ctggacttct catacagaca
     9241 caccagcgcc tcgaaggaat cgagattctc gggagccagc gaaagaatcc gcttgtgagc
     9301 ctcggccgcg cgatccagat tgttgagtcg attttcggcg acatccgcca tcagcaggtt
     9361 caagcgaatc cgatcacggt cgctgtcaac gaccgacaat tcaaattgcg cggtcgcaaa
     9421 ctgcttctgc caatccgact tgcgaccgta atagccgatg tagaattcca gcgcctcgat
     9481 gttgcgcgga tccagggcgc gcagacgacg ccacagtttc tcggcgtcgt cgtcgcgacc
     9541 cgacagcccg agcaggcgcc cgagactggt caggagaggg atttccgaag gagtcccggt
     9601 caacgctcgt cgaacggttt ccagaaaggc ggccccgcgc tcggacagcc ccccttcttc
     9661 agcaatggcc acataacggt ccagagcctc gcgactggtt ctgtcggcct ccagcgcggc
     9721 ctcgagcgcc gtcatcgcgg cttccgtacc gccacccatg agatgcagcg cggcaagatc
     9781 gacaagggcg gcagccttgt cggcatcatg aagaccggcc gcgatgcgcg aaatgaactt
     9841 gactgcgcgt tcggccactt cctgttcaat cgccaaacgg atgtaggccc cgcgaagatc
     9901 gcgatccttc ggggccctgt tcagaccggc ctcaagaccg gcaaggtcgg ccgggacgga
     9961 aacgtcagca tccgaggaac cggcgtcgcc actgtccgca tcctccgcgg gctcgctgga
    10021 agcgatgccg tcggcttccc gatcctccgg ttcaaactga agcgtctgct ggcgctgagc
    10081 ggggtcgtcc agatcaggac tggaatcgtc gccggcatcg acgttctttt ccggtgaaat
    10141 cccggcatcc tcggaaatcc cggcatcctc ggaaatcccg gcatcctcgg aaatcccggc
    10201 atcttccgaa atcccgacat cctccggaaa tacatcctcc tgcataacct gctcgactcc
    10261 atccgcggga gtcgcttcgg ccgccatccg ggcacgacgc gccgcaaggg cttcgccaag
    10321 cgtcggccca taacgacggt tgtagtccgc gtcgccgcag ccggccagaa gacgaagcag
    10381 ccgtatcttc acatcctcat caagcccctg gccgaaaagg tcgtacacgt ccgccagcgc
    10441 agccggattc agactgagaa tcgcttcggc aagccttgcc acctgcgcca gggactgatc
    10501 accgacgtcc tggtcgaaaa gatcggccgc cgcaccggca atccgtgaaa cggagttcct
    10561 tgcggtcttg tcggcctcgg catcgaacgg attgtccaac gccgccccaa gcagcaggtt
    10621 cgcgacgacg gcggccagcg aatgaaccat ttccggcccc gacgcctcga gcgccttttc
    10681 gcgaataagc gaaagtgttt caataagttc ggcaccgctc atgtcggcgc cgcattcccg
    10741 agcaaccgaa aaatccggag tgtcggaaaa tatgatctcg aacatcttgt ccaggctgga
    10801 tgtgctcatc ggctgcgatg ctccttagtg ccctctgcgg gtcgtacttt tttacccttt
    10861 acacggtata ttggcaacag attatgcacg tacgtacgcg tgcacggccg catttgacgc
    10921 gccggggggc ggcacgaccg ccaacctctc caaatatcct gttcacaatc actcgcggcc
    10981 cttcaacgaa cgaccggatg ctgttaaatt tgcgatggca gacaagactg ggttccggta
    11041 tccggaggcg ccgatgtcct ttgttcatct tcatcttcat tcgcactaca ccctgctgaa
    11101 ttcaacaatc cggattacgg atctggtcga caaggccgtt tcaagcggcg tgcccgcggt
    11161 tgcccttacc gacaccatga acgtgttcgg cagcctggaa cttgaaaaga aaatacgggg
    11221 aaagggtgtc aaaccgattt acggcgccga actgctgatt tcgaccgatc cgggtgtcgc
    11281 ccccttcagc ctggtcgtcc tttgcaggaa tcgcgagggg ttcgccaatc tgcgcgaaat
    11341 cctttcacgc gccgccacgg aatcgccccg ggacgattgt cactgcacga ctcgcgacat
    11401 actggcatcc ctttcgaaag gtctcatcgg gctctccgga agcctcggcg ggcagattcc
    11461 ccatgccatc ctgactggcg gtcaggccga acaactggcc tccgaatacg catcgatttt
    11521 cgaacccggc tgtttcttcc tcgagttgca gggcaacgat ctcgaggagc agaagacagt
    11581 caaccgtgaa ctggcagcga tcggccagcg catcggcctt ccctgcgttg cgacgaacaa
    11641 cgtccattat cttgaacgtt cggatgcccc ggcacacgca atcctcgtcg cgatcggcca
    11701 gcgcaagcgc ctttccttcg agtccctgga caacttcccg ctggactcgt ttcacttcgc
    11761 gtccgaggcc gacatgcggc gggccttttg ctggattccg gaagccgttg acaatacggt
    11821 gaaaattgcc gaaatgatcg agccggacat cctgttttca tcccagaagc acttctttcc
    11881 ggtctatcag ccgcccgagg ggatgacggt cggcgagtac cttacgaaac tggcgaagga
    11941 aggcctcgcg gacaggctgg ccaattcccg caagaccggc ttcgaaaagg atccggcgga
    12001 gtactgggca cgactggatc gggagttgga agtcatcatc ggccagcact tcgacaccta
    12061 ctatctggtt gtatgggact tcatcaactg gagccggcag aacgacgtcc cggtcggccc
    12121 tggacgcggg tcgggcgcag gctcactggt cgcatactca atcggcataa ccgatgtgga
    12181 tcccctctcg tacgacctgc ttttcgagcg gtttctgaac tcggaacgca tatcgccccc
    12241 cgacttcgac atcgacttct gcaaggacga acgttccaga accattgaat acgtcaccaa
    12301 ccgttacggc cgcgaacagg tgtgccagat cgtaaccttc ggcacgctga aggcgcgggc
    12361 ggcaatcaag gacgccgcgc gcgttctggg cttcagcttc gatgaaagcg acgtcctgac
    12421 caagatgatt ccggaggagc ccgggatgac ccttgccagg gcggccgaga tcgattcccg
    12481 catcgagcag gtgtgcaagt ccgacgctcg tttcggccgt ctgtgggaag tcgcgctgaa
    12541 gctggagggg ctggccaggc agaccggcaa gcatgccgcc ggcgtggtca tcgcggatcg
    12601 accggtcaag gaatacgcgc cgctgtacgt caacgacgac ggctcaatcg tcacccagta
    12661 cgacatggcc aacctggaca cggtcgggct gatcaagttc gacttcctgg gattgctgtc
    12721 cctgacgata atcgcccacg cactgaagaa catccgggca cgcaaggatc ccggattcga
    12781 tctgggccgc attccgcttg acgatcgcgc cactttcgac ctgctctgca gcggacggac
    12841 ggccggagtc ttccagcttg aaacaagggg aatcacgaac caggtaaagc ggcttcgccc
    12901 agataccctc gaagatatcg tcgcgactat cgccctcttc cgtcccgggc cgctgcaaaa
    12961 caagatggac gaggaattca ttgcggtaaa gcacggccag aagaaagccc agtatgctca
    13021 cccgctgctc gaaccaatcc tcaaggacac ctatgggacc atcctttacc aggaacaggt
    13081 gat
//
LOCUS       DAOPPA010000020        13069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5549_length_13069_cov_3.699247, whole genome shotgun sequence.
ACCESSION   DAOPPA010000020 DAOPPA010000000
VERSION     DAOPPA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5549_length_13069_cov_3.699247"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PKH54_01270"
     CDS             <1..1013
                     /locus_tag="PKH54_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98544.1"
                     /translation="GCDEDIQCDDLSACTGTESCINSTGQCVPGRALECDDNRSCSAD
                     WCDITAGCLVDPSGCPCVSDDQCDDGSVCTGREFCDPVRGRCVAGAALDCRDGRPCTR
                     DSCDRHTGCVNEPSSCRCADDGDCKLPYEDCDPASGRCRNMVCASCSGDTDCGFPGNV
                     CHRFDSGMACVLDCAPGDFICPVGYECRDEGFARLCMPVAGDCKCEPAAALTCHAGNL
                     WWFDGCGQPDRVAVDCGERGCFDRECCLEGESASEGGCRLVVEHQGDAGEPDIPDPED
                     VQSDSGGVADMEIDVPAGGCDATSGNPANPLKAVIPVLVVAALSLLAIGRFRRFDRPS
                     RG"
     gene            complement(1019..3379)
                     /locus_tag="PKH54_01275"
     CDS             complement(1019..3379)
                     /locus_tag="PKH54_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOC98545.1"
                     /translation="MDFFRLFLSVALPACLLALPLSAAPLKDIPSRFEQPDGTVVMLL
                     TSGDEFSRVTTDLKGFTVVRDFATGWLHYADLIDGKLAPTILAAGIDDPAAAGLQPGL
                     RPTLRESRRNPGVRRVARAGDVTPSVGQINNLFVFIRFSDDETMYEESDLYDEKFNST
                     ADGHASMRAFFSEVSMGAVDVRTIFTPPPDGAWMTAWTSPKSRQYYQPYDARSNTQGY
                     SGDMEAQMREHAMLRDALAGIEDLIPPNIDFDANDDGWFDNVIFMIQGWPDGWSDLLW
                     PHKWEFYEAPTVAGLQVGTYNLQLSVLMRVSPGTFAHEMYHSFGAPDLYHYSQDGRVP
                     VGPWDLMEATGMEAAQHPLAYMKWKYGNWIEDIPIVSENSDITLHSSWMNGTQAVRIP
                     VPESDKEYFVVEYRRRLGLFETQLPSSGLIVYRINPDLEGIGNREGPPDEVYVFRPGE
                     GPKTNGSLKDATMKSGGRTVMNDVTDPPLFLKDGTTVELRIYDVGEVGDTISFKVCLV
                     APGCGELVCGSDGFGGWCGDCPAGNWCLGGACVPCTCDGRDCGDDGCGNSCGTCDDQD
                     QCTTDECVDYKCVFTISDGLACDDGDLCTSDDMCSALGACAGTPYTCSPGRCMSDSTC
                     KGDGTCKPVWAPLGTSCDDNDPCTMNDACNDKKKCVGEQYACLPGPCDSAADCDGTGG
                     CQATFMAVGTPCDDSNPETVDDACTPEGVCAGQVPQTHDVIEDANGNADTVQATDEGE
                     KPVEETAPASGGCSASDGIPGAAWLPLAIALGYLLSSRRRFDRRAR"
     gene            3519..4580
                     /gene="rsmH"
                     /locus_tag="PKH54_01280"
     CDS             3519..4580
                     /gene="rsmH"
                     /locus_tag="PKH54_01280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOC98546.1"
                     /translation="MDDGDRDSVEGVDDVAGGHRRRPRYSGTHPRRFDQKYKELDPSV
                     DPDTVARVLASGRTPAGMHRPIMVREIIGVLQPAPGCLVVDATLGWGGHSGAIIPMIV
                     PGGHLFGLDCDPVELPKAESRLRLAGWGPDSFSAHRINFSGISRILDDAGVDAVDCVL
                     ADLGLSSMQIDDPSRGFSVKQDGPLDMRMNPSRGITARALLEKIRPERLASILAENSD
                     EPEAEAIAAAVAGRSFSGTAELARTVRQASGHREREQQEMAVRRVFQAIRIEVNEEFS
                     ALDSFLRALPACLKPGGRVAILTFHSGEDRRVKKSFKAGLLDGTWSRIADEVVRASPA
                     EQRDNPRSAPAKLRWAVRS"
     gene            4664..5857
                     /locus_tag="PKH54_01285"
     CDS             4664..5857
                     /locus_tag="PKH54_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98547.1"
                     /translation="MKAHSICVLFIAVLVVGGCSGNGEGPAADSGNPDTVLPDNGQDA
                     SPPDNGQDTGSTPGKRVYGGSASYGDLVVFSVDGDSRKVEVVNETTGNTVSNDYTVMS
                     GNVEGVLKVESAGDTFFAVELTNKLLAASFDTGNPSNTISFGVAGGTDNIANQSNIPG
                     DYAYIHISGQPVNGSTDIKEWGVLSIIDDGTFRVKAFASDAGDMSLGPMAPEEWAGAF
                     PVEEPNVQGSWSVNPSDHARLVVNIDGVPATLTGFAYAPDPNGIFVLDMGTGNGFILA
                     MKILPTMTLANLAGEYKFINVWNDSMGEGRSAGHAVVNSDGTGFFGTVDTDGGYSTGT
                     FDYISACPNLQNMFHATSTETRDDVTVTLKHYFVLLPYAYMGFNFRTDGGFRFVGYSV
                     GARLP"
     gene            complement(5869..7578)
                     /locus_tag="PKH54_01290"
     CDS             complement(5869..7578)
                     /locus_tag="PKH54_01290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOC98548.1"
                     /translation="MPSLMTKLGVYKRVLTWAINKNKIDIDYKPPTDNPRFKTGMEAA
                     AMIPDGAIIAGCGMASNVRPASVYKAVRESFIKTGHPCNLTWINPGGTGLRGKAPGGT
                     EEVCLPGLLTHYISGHHETVRAGLELVAKGEMYLTVYPQGTLIHLIDGQGRGEYDLLT
                     EVGVGTFVDPRVGTGTQVEPGRGEQMVTAEGDKLRFTLPKITCSVVAATYADKDGNIY
                     MKDSPMFTEVRESTRAARNNGGLTIVNVAGIIPRDDANIFIPADEVDCIVVNRYAEQA
                     VMFPQKKALLGLTPGHKMKDIPKFLDELNMFNKILKLDPIRNPIEYMLARQAASVYTG
                     VGFPGVKTIIGYGLPQEVGAMASRGGIAKDITYLIETGVIGGIPAPGFFFGMAINPEK
                     LVSSAEMFHFIEDNLDVAILGMLQVDPQGNVNVSRKSQKVKEYVGPGGFINLVTYAKN
                     LIFIGQWTAKAEMQIVDGKLQVTKRGIPKFVEKLEEITFSAQNALKRGQNVFYCTTAG
                     TFRLTERGIELFQIADGLDIQKDILDVAPWARIVLPEDGKVQVLDRSIMTGEGFKLAW
                     KNG"
     gene            complement(7675..8613)
                     /locus_tag="PKH54_01295"
     CDS             complement(7675..8613)
                     /locus_tag="PKH54_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC98549.1"
                     /translation="MTDDLTPTSGPVICWGELLWDVIKDAERDVEVPGGSPLNVAFHL
                     NRLGHTAYPVSAVGDDRRGRALLDFMDGHGIPRDHVGIVFGLPTGVARVEAGPDGLPL
                     FSIDEVSAWDRIPIGPHLEKLAAGAKAIVFGSLAQRGPANLDSLISLLESTSALKVMD
                     ANLRPPFDDHRTVLQLARQADILKVSESELHSLLASWSDQVGRDGTRGNDDIRAAAIE
                     LSRFTTVPLIAVTFGSDGAAILDHDAWHFQRAANVPVEDTVGAGDAFLAGLLAGHLAG
                     LPWEGALAGACRTAEATVGHRGAIPSSRFPASPGKG"
     gene            8857..10692
                     /locus_tag="PKH54_01300"
     CDS             8857..10692
                     /locus_tag="PKH54_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98550.1"
                     /translation="MKKIFVLILALAAAGCGAGETGSDVLNDVARDAVSDVAADTAGD
                     VAADTAGDVAGDVAADISAEVADDVASDTADDAGPDTGSGWKVPAEEWSSCPQVEYIG
                     SKTLAQKAAYYDWAGMKLHQKPWDAPGHEDYSTVYEINCDADVPTEIVPDGQIPNCVN
                     PLSENTGLWTSLYVASQAFRYATTHEAEALATLKRTLNGTYHQLLITGTPGLYTRDMR
                     DPTLPSQFCVEDEEPYKSAATDNGKYARYVPRPDDTMVGNQFVRVDTDGCFLTWDPAL
                     NEGAGGWLRHENYCTDPKFAGFCWQDNASKDEYAGHMFAAGIVAMIVDDADVRAMAVD
                     ILRSVAMHMVEHDFMITDYDGRRTRFGSAFALAFDEAPGGNAVMALSWIRMAATVTGD
                     QVLIDTYYDCLLQMSGKLQCIEQPFEWDSPADYTTYFADQGMDLAKGSKSNYDTINIA
                     LLNWFNLVWFEPGRDLRDIYRQAFRDNTYGPDREERDLWDQANPFFNFSVVSRMESDD
                     YDQTEVKKLVEDSVCTLKQFPTDNIRRAKDSTVYPEWKVSPRHGSLAESAIPISERCS
                     SVFEWWGDPNSRETCAENLRNAEPPAGYLLPYWMGRYFGFISQDL"
     gene            10772..>13069
                     /locus_tag="PKH54_01305"
     CDS             10772..>13069
                     /locus_tag="PKH54_01305"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOC98551.1"
                     /translation="MARIAFVRAALSAVVLVWLFVASGCGGGEDSGDLTGDQGFVDDV
                     VGELAAVDVASDHRTDTPVDVADDPGTIDAIDPDAIDADSADSSGDDGDVAGDVVDED
                     VMPDVPRRDAEWDDVPVPPPSFPIVRDGSATHVIVVADEASQSEKTAAAEIQSIIVQA
                     TGVELQMAAVPPGGDVPMIVVGCGLAASGLGVTEEPDSFDEGEYLIRVVGNSVVIAGN
                     PAAGTMHGVHEFLKAAAGVRWTAPGVTVVPQASEIVFPVQYDRRYKSPFKWRTTTYEW
                     PGADREFWAHQAENSGSSGPDAAFGAEHWHDGIAHSYFSFISPDEFWDTHPEFFSEIG
                     GVRVREETQLCLTNPDVLDIVTERMLARMAAHPDAMQHNFSQMDHYNQCQCDKCRAMN
                     ELYQSEGGTQFWFVNELAKRTSLEYPDKQIGTLAYMYTEEPPVGLELHPNVAVWLCHM
                     YPSCDSHPIRTCPDNADYKRRAQAWAELTDHLYIWHYIVDFMHYYNPFPNFWALADNI
                     RFYRDIGVEGVFLQGMGHQGGGGEFSLLRPWFGMQLLWNPDQDVTALIKRFLKDYYGP
                     AWGPIWDWIVLLQAKVDDEDIHMHLYSNPAQGYLPEDIVVTGERLFAEAAALVEGDPI
                     LEDRVDVARMPLLYARFFPRNGYTISDGWLRWNPGMATWDDVNLFLQMMNDHGFTSYR
                     EAQGSPDTMTMLYMMIGADAEVKTISNGKLSVDVVPTLAGRALRITDVASGQTVTAWD
                     QRRNLFYPFQGGLEDRVGEGFNFMGW"
BASE COUNT         2469 a         3865 c         4075 g         2660 t
ORIGIN      
        1 gcggctgcga tgaagacatc cagtgcgacg acctttctgc ctgcaccgga accgaaagtt
       61 gtatcaattc gaccggccag tgcgttccgg ggcgggctct tgaatgtgac gacaaccggt
      121 cgtgttccgc cgactggtgt gacatcaccg ccggctgtct tgtcgatccg tccggttgtc
      181 cctgcgtttc cgacgaccag tgcgacgacg gatccgtctg caccggcagg gaattctgcg
      241 atcccgtcag gggacgctgc gtggccggtg ccgcgctgga ctgccgggac ggccgtccat
      301 gcacacggga ttcatgcgat cgccacaccg gatgcgtcaa cgaaccgtcc tcgtgccggt
      361 gcgccgacga cggcgactgc aagttgccat acgaggactg cgaccccgca tccggacgat
      421 gcaggaacat ggtctgcgca tcatgttccg gcgacaccga ttgcggcttc ccggggaacg
      481 tatgtcatcg cttcgattcc ggtatggcct gcgtccttga ctgcgccccc ggcgatttta
      541 tctgccctgt cggttatgag tgccgggatg agggcttcgc caggctgtgc atgcccgttg
      601 ccggtgactg caagtgtgag ccggcggcgg cgctgacttg ccatgcaggc aacctgtggt
      661 ggtttgacgg atgcggtcag ccggatcggg tcgccgttga ttgcggcgaa aggggctgtt
      721 ttgacaggga gtgctgcctt gaaggtgaat cggcgtcgga aggcggatgt cggcttgtcg
      781 tcgagcatca gggcgacgcc ggagaaccgg acattccgga tccggaagac gtccagtccg
      841 attccggagg cgttgccgac atggaaattg atgttcccgc cggcggatgc gatgcgactt
      901 cgggaaatcc cgcgaatccc ctgaaggcgg tcattccggt tcttgtcgtg gcggctttgt
      961 cactgcttgc aatcggacgt ttcagacggt tcgatcgccc ttcgcggggt tgaccaggtc
     1021 agcgcgcgcg ccggtcgaag cgacgccgtg acgaaagcag gtatccgaga gcgatcgcca
     1081 gcggcaacca cgcagcacca ggaatcccgt ccgaagccga gcagccccca gatgccggcg
     1141 ccgtctcctc gacaggtttt tcaccctcat ccgttgcctg gactgtgtcg gcgttgccgt
     1201 tggcgtcctc gatcacatca tgggtctgcg gcacctggcc ggcgcaaacc ccttccgggg
     1261 tgcacgcgtc atcaaccgtt tccgggttgg agtcgtcgca cggagtaccc acggccatga
     1321 acgtcgcctg acagccgccg gtgccgtcgc agtcggcggc gctgtcgcat ggtccgggca
     1381 ggcatgcgta ctgttctccg acgcattttt tcttgtcgtt gcatgcgtcg ttcatcgtgc
     1441 acgggtcgtt gtcgtcgcac gaggtcccaa gcggggccca tacgggcttg catgtaccat
     1501 cgcccttgca ggtggaatcc gacatgcatc ggcccggcga gcaggtgtat ggagttcccg
     1561 cgcaggcgcc aagggcgctg cacatgtcgt cgctggtgca caggtcgcca tcgtcgcacg
     1621 ccagcccgtc ggaaatcgtg aagacgcact tgtaatcgac gcattcgtca gtcgtgcact
     1681 gatcctggtc gtcgcatgtg ccgcacgaat tcccgcaccc gtcgtccccg cagtcgcgtc
     1741 cgtcgcacgt gcacggaacg caggcgccgc caaggcacca gtttccagcc gggcagtcgc
     1801 cgcaccagcc gccgaatccg tcgcttccgc acacaagttc gccacatcca ggcgcgacca
     1861 ggcacacctt gaacgagatc gtgtcgccga cctctccgac gtcgtaaatc cgaagttcaa
     1921 ccgtcgtccc atctttcagg aacagcggcg gatccgtcac gtcattcatg acggttcttc
     1981 ccccggactt catcgtggcg tccttcaggc tgccgttcgt ctttggtccc tcgccgggcc
     2041 ggaagacata aacctcgtcc ggcgggccct cgcggtttcc aatgccttcc aggtcggggt
     2101 tgatgcgata cacgatcagt cccgacgatg gaagctgggt ctcgaaaagg ccaagacgac
     2161 gccggtattc gacgacgaag tactccttgt ccgattccgg gacgggaatc cttaccgcct
     2221 gcgttccgtt catccacgag gaatgaaggg tgatgtcgct gttttcggaa acgatcggaa
     2281 tgtcctcaat ccagttgccg tacttccatt tcatgtacgc gagagggtgc tgggcggctt
     2341 ccatcccggt cgcttccatc agatcccacg gcccgaccgg cacgcgcccg tcctggctgt
     2401 aatggtaaag gtcgggcgcg ccgaacgaat ggtacatttc gtgcgcgaag gttccgggcg
     2461 aaaccctcat caggacgctc aactgaaggt tgtaggtgcc gacctgcaag cccgcgacgg
     2521 tcggagcctc gtaaaattcc catttgtgcg gccacagcag gtcggaccag ccgtcgggcc
     2581 agccctgaat catgaaaatc acattgtcga accagccgtc gtcgttcgca tcgaagtcga
     2641 tgttgggcgg tatcagatcc tcgattccgg ccagtgcgtc gcgaagcatg gcatgttcgc
     2701 gcatctgggc ttccatgtcg cccgaatagc cctgggtgtt cgacctggcg tcatacggct
     2761 ggtagtactg ccttgatttg ggtgacgtcc acgcggtcat ccacgccccg tccggtggcg
     2821 gggtgaagat ggtccggaca tcgaccgcgc ccatcgacac ctcgctgaag aacgcccgca
     2881 tcgaggcgtg gccgtccgcg gtgctgttga acttttcgtc gtacaggtcg ctttcctcgt
     2941 acatcgtctc atcgtccgaa aagcggatga acacgaagag gttgttgatc tggccaacag
     3001 atggggtcac gtcaccggcc cttgccacgc ggcggactcc cggattccgg cgcgactccc
     3061 gcaacgtcgg tctcagtccg ggctggagtc cggccgcggc cggatcgtcg atccccgcgg
     3121 cgaggatcgt tggggccagc tttccgtcaa tcaggtcggc ataatgcagc cagcccgtcg
     3181 cgaagtcgcg gacgacggtg aaacccttca gatccgtcgt gacgcgtgaa aattcgtcgc
     3241 cagaggtcag gagcatgacg acagtcccgt cgggctgctc gaagcgggac ggaatgtctt
     3301 tcaacggcgc cgcggacagc ggcaaagcca gtaagcaggc cggcagggca acggacaaaa
     3361 aaagcctgaa aaaatccatg acaactccgc aatccattgt gaaagaaacg cgaagtgcag
     3421 tttaccatta cctgtttgtc cgttgacagt cccgttgtgt tgattcctaa tcgggcccgt
     3481 cacgcgaccg ggcaggacgt ggcccggggg aaggattgat ggacgacggc gacagggatt
     3541 cggtcgaagg cgtcgatgat gtcgcgggtg gtcaccggcg gcgaccaagg tactcaggga
     3601 ctcatccaag gcgttttgac cagaaataca aggaactgga tccgtctgtg gatcccgata
     3661 cggttgcaag ggtgctggct tcgggaagga ctccggccgg gatgcaccgc ccgatcatgg
     3721 tgcgcgagat aatcggggtt cttcagcccg ctcccggatg ccttgtggtt gacgccacgc
     3781 tcggctgggg cgggcactcc ggggcaatca tcccgatgat tgttccaggc gggcatctgt
     3841 ttggactgga ctgcgatccg gtcgagcttc caaaggccga gtcaaggctt cgtctggccg
     3901 gctggggccc cgattcgttt tcagcccatc ggatcaattt cagcgggata tccaggatcc
     3961 ttgatgacgc cggcgtcgat gcggttgatt gcgttctggc cgacctgggg ctttcgtcga
     4021 tgcagattga cgacccgtcc cgcgggtttt ccgtcaagca ggacgggccg ctggacatgc
     4081 gaatgaatcc gtccagggga attaccgcgc gggcgttgct ggaaaagatc aggcccgaac
     4141 ggcttgcatc aatactggcg gagaattcgg acgaaccgga agcggaagcg attgcggccg
     4201 cggttgccgg ccgttcgttt tcggggaccg ccgagctggc acggacggtt cggcaggcct
     4261 cggggcaccg tgagcgcgag cagcaggaaa tggcggttcg ccgcgtgttc caggcaatcc
     4321 ggattgaggt caacgaggag ttttcagccc ttgattcgtt cctgcgagcc ttgccggcct
     4381 gccttaagcc tggcggcagg gttgccatcc tgacattcca ttcgggcgag gatcgaaggg
     4441 tcaagaagtc gttcaaggct ggcctgcttg atggaacgtg gtccaggatc gccgacgagg
     4501 tcgtgcgcgc cagtcccgcc gagcagcggg acaatcccag atcagcgccg gcgaagctgc
     4561 gctgggccgt ccgctcctga ttccgttaaa gggcccttac aaaaatttcc ggtcacgaat
     4621 ggacgggatt atcatcgggc cgaatttcaa ccctcggtgg agtatgaaag ctcactcaat
     4681 atgcgtgctg tttatcgccg tccttgtagt tgggggatgt tccggaaatg gggaagggcc
     4741 cgcggctgac tccggcaatc cggataccgt gctgcccgac aacggtcagg acgcgtcgcc
     4801 gccggacaac ggtcaggaca cggggagcac cccaggcaaa agggtgtacg gtggttcggc
     4861 ctcgtatggt gacctggtcg tgttttcggt tgacggagac tcgcgaaagg tcgaggtcgt
     4921 caacgagacc acgggcaata cggtcagtaa tgattacacg gtgatgtccg gcaatgtcga
     4981 gggcgttctg aaggtcgagt cggccgggga taccttcttc gcggtcgaac tgacgaacaa
     5041 gctcctggcg gccagttttg acaccgggaa cccatcgaac acgatttcct tcggggtcgc
     5101 cggcggcacc gataacatcg ccaatcaatc gaacattccc ggcgactatg cctatatcca
     5161 catatccggc cagccggtga acggatcgac ggacatcaag gaatggggtg ttctttccat
     5221 catcgacgac ggtaccttca gggtcaaggc attcgccagc gacgcgggcg acatgagcct
     5281 tggaccgatg gctccggagg aatgggccgg agccttcccg gtcgaggagc cgaacgtcca
     5341 gggttcctgg tccgtgaatc catcggatca tgcgcgcctg gtcgtcaata tcgacggggt
     5401 gccggcaacc ctgaccggat tcgcgtatgc tcccgatcca aatggaattt ttgttcttga
     5461 catgggtacc gggaacggat tcatccttgc catgaagatt ctgccgacca tgacgcttgc
     5521 gaatcttgcg ggcgagtaca agttcataaa cgtatggaac gacagcatgg gtgaaggccg
     5581 cagcgccgga cacgccgtgg ttaactccga tggtaccggc ttcttcggca cggtcgatac
     5641 cgacggcgga tattcgaccg ggactttcga ttacatcagc gcctgtccga accttcagaa
     5701 catgttccac gcgacttcaa ccgaaaccag ggatgacgtc acggtcaccc tgaagcatta
     5761 cttcgtcctg cttccgtatg cgtacatggg attcaacttc aggactgacg gcgggttcag
     5821 gtttgtcggg tattcggttg gcgcccggct gccgtgacaa acgcgggtct atccgttctt
     5881 ccaggccagc ttgaatcctt cgccggtcat tatcgagcga tccaggacct ggacctttcc
     5941 atcttcggga agaacgattc tggcccacgg ggccacgtcg agaatgtcct tctggatatc
     6001 gagtccgtcg gcgatctgga acagttcgat tccgcgttcg gtcagcctga aggtgccggc
     6061 cgtcgtgcag taaaaaacat tctggccgcg cttcagagcg ttctgcgcgc tgaaagttat
     6121 ctcttccagc ttttcgacga atttcgggat gccccgcttc gtgacctgga gcttgccgtc
     6181 aacgatctgc atctctgcct tcgcggtcca ctggccgatg aatatcaggt ttttcgcata
     6241 ggtgaccagg ttgatgaagc ccccggggcc cacatattcc ttcaccttct gcgatttgcg
     6301 gctgacgttg acgttgccct gcggatccac ctgaagcatt cccaggatgg cgacgtcaag
     6361 gttatcctcg atgaaatgga acatctcggc ggacgacacc agtttttcgg gattgattgc
     6421 catcccgaag aagaatccag gtgccgggat tccgccgatg acgccggttt cgatcaggta
     6481 cgtgatgtcc tttgcgattc cgccgcggct tgccatggcg ccgacttcct gcggcaggcc
     6541 gtacccgatg atcgtcttga cgccggggaa tccgacgccg gtatagacgc ttgccgcctg
     6601 gcgcgcgagc atgtactcga tcgggttgcg gatcggatcc agtttcagga tcttgttgaa
     6661 catgttcagt tcatcaagaa acttcgggat gtccttcatc ttgtggccgg gggtcaggcc
     6721 gagcagcgcc tttttctgcg ggaacatgac ggcctgctcc gcgtacctgt tgaccacgat
     6781 gcaatcgact tcgtccgcgg gaatgaagat gtttgcgtcg tcgcgcggaa tgatcccggc
     6841 gacattcacg atggtcagtc cgccgttgtt gcgggccgcc cgcgttgatt cgcgaacttc
     6901 ggtgaacatc ggcgaatcct tcatgtagat gttcccgtcc ttgtcggcat aggtggcggc
     6961 gacaacggag caggtgatct tcgggagggt gaagcgcagc ttgtcgcctt cggcggtgac
     7021 catctgttcg ccccggcccg gctcgacctg tgttccggtt ccgacccgcg gatcaacgaa
     7081 cgtgccaacc ccgacttcgg tcagaaggtc gtactcgccg cgtccctggc cgtcgatcag
     7141 gtggatcagc gttccctggg gatagacggt caggtacatc tcgccctttg caacaagttc
     7201 aaggcctgcc cgcacggttt cgtggtgtcc gctgatgtag tgggtaagca ggccgggaag
     7261 gcagacttcc tcggttccgc ccggcgcctt accgcgaagg ccggttccgc ccgggttgat
     7321 ccaggtaaga ttgcacgggt gtccggtctt gatgaaggat tcccggacgg ccttgtaaac
     7381 cgatgcgggc ctgacgttgg acgccatccc gcagccggcg atgattgcgc cgtccggaat
     7441 catcgccgcg gcctccatcc cggtcttgaa cctgggattg tccgtcggcg gcttgtagtc
     7501 gatgtcgatc ttgttcttgt taattgccca ggtcagaact cgtttgtaaa cgcccagttt
     7561 tgtcatgaga cttggcatct atccggcctc cttctggaaa gcttgagtga acccgatata
     7621 tgtgccatca atcatgccgg caagtcaaca gcggtgtcag cttatcggcg tccgtcagcc
     7681 tttgccgggc gacgcgggga atcggctcga cggaatggcg ccacggtggc cgaccgtggc
     7741 ctcggccgtc ctgcatgcgc cggcaagcgc tccttcccag ggcagtccgg caagatgtcc
     7801 tgccagcagt ccggccagga aggcgtcgcc cgcgccgacg gtgtcttcga ccgggacgtt
     7861 cgccgctctt tgaaaatgcc aggcgtcatg gtcaaggatt gcggcgccgt cactgccgaa
     7921 ggtcacggca attaacggga cggtcgtaaa cctggaaagt tcaatggcgg ccgcgcggat
     7981 gtcgtcgttg ccgcgagtgc cgtcgcggcc gacctggtcg gaccaggaag caagcagtga
     8041 gtgaagttcg ctttccgaga ccttcaggat gtcggcctgg cgggcgagtt gaaggacggt
     8101 ccggtggtca tcgaaaggcg gacgaaggtt tgcatccatt accttcagcg ccgaagtcga
     8161 ttcaagaagc gatatcagcg aatcaaggtt ggccggcccg cgctgggcca ggcttccgaa
     8221 gacgatggcc ttcgcgccgg ccgcaagttt ttcaaggtgc gggccgatcg ggatcctgtc
     8281 ccatgccgac acttcatcaa tactgaacaa ggggaggccg tccgggcctg cctcgacccg
     8341 cgccacgcct gtcggaagcc cgaaaacgat gccgacgtga tcgcgcggta tgccgtgccc
     8401 gtccatgaaa tcaagcaagg cccgtccccg ccgatcgtcg ccgacggcgg aaacgggata
     8461 ggccgtatgg cccaggcggt tcaggtgaaa tgcaacattc aggggagagc cgccgggaac
     8521 ctcgacgtcc ctctctgcgt ccttaatgac gtcccacaga agttcgcccc agcaaattac
     8581 cggcccggac gtcggtgtga ggtcgtctgt catgaccctg tcatccagaa aggttcccgc
     8641 cgcctcaagg tgccaacagg tcagggagcg atcagcgaat cataaacctc ggcaaggatg
     8701 attcctgaaa tttccgaaca ccaatttttg tacgatgtca gtaactgtca aacaatcatt
     8761 tttgccccgt cgcggtcgat ttggtaacct cgcccttctg tcctgcccga ctgcgggccg
     8821 gaatgccgga ttcaattaat tgccaggatg attctgatga agaagatttt cgtgttgatt
     8881 cttgctcttg ccgcggctgg atgcggggcc ggtgaaacag gaagcgacgt cctgaacgac
     8941 gtggcgcgcg atgcggtcag tgacgtggcc gccgacacag ccggggacgt ggccgccgac
     9001 acagccgggg acgtggccgg ggatgtggcc gccgatattt ccgccgaggt tgccgatgac
     9061 gtcgcctccg acacggccga tgatgcgggg ccggacactg gttccggctg gaaagtcccc
     9121 gccgaggaat ggtcgtcctg tccccaggtc gaatacatcg gctcgaagac gctggcgcag
     9181 aaggccgctt attacgactg ggccgggatg aaactgcacc agaagccgtg ggacgccccc
     9241 ggtcacgagg actattcgac ggtttatgaa atcaactgcg acgcggacgt gccaaccgaa
     9301 atcgttccgg acgggcagat tccgaactgc gtcaatccac tttccgaaaa caccggtctg
     9361 tggaccagcc tttacgtcgc atcccaggcg ttccgatatg cgacgaccca tgaggccgag
     9421 gcgctggcca cgctgaagag gacgttgaac ggcacctacc accagttgct gattacaggc
     9481 acgccggggc tttacactcg cgacatgcgc gacccgaccc tgccgtcaca gttctgcgtc
     9541 gaggacgagg aaccgtacaa aagcgcggcc acggacaacg ggaagtacgc caggtacgtg
     9601 ccgcgtccgg acgacaccat ggttggcaac cagttcgtgc gggtcgatac cgacggttgt
     9661 ttcctgacat gggatcctgc cctgaacgaa ggggccggtg gctggttaag gcatgaaaac
     9721 tactgcaccg acccgaaatt cgcgggcttc tgctggcagg acaacgccag caaggacgaa
     9781 tacgcgggcc acatgttcgc ggccgggatt gtggccatga ttgttgatga cgcggatgtc
     9841 cgcgcgatgg cggtcgatat cctgcgaagc gtcgcgatgc acatggttga gcacgatttc
     9901 atgatcaccg attacgacgg gcgccgcacc aggttcggct cggccttcgc gctggcgttt
     9961 gacgaggctc cgggcggcaa cgcggtgatg gcactgtcct ggatccggat ggccgccaca
    10021 gtgaccggcg accaggtgct tatcgacacg tactacgact gcctgcttca gatgtccgga
    10081 aagcttcagt gcatcgaaca gcctttcgag tgggacagcc ctgccgacta caccacgtat
    10141 ttcgcggatc agggcatgga cctggcgaag ggttcgaagt cgaactacga cacgataaac
    10201 atcgcgctgc tgaactggtt caacctggtc tggttcgagc ccgggcgtga tcttcgcgac
    10261 atctaccgcc aggcattccg cgacaacaca tacgggccgg atcgggaaga gcgcgatttg
    10321 tgggatcagg ccaacccgtt cttcaatttc agcgtggtca gcaggatgga atcggacgat
    10381 tacgaccaga ccgaggtgaa aaaactggtc gaggattccg tctgcacgct taaacagttc
    10441 ccgaccgaca acatcaggcg cgccaaggat tcgacggtct atccggaatg gaaggtgtcc
    10501 ccgcgtcatg gcagtctggc cgaaagcgcg atcccgatat cggagcgctg cagttcggtg
    10561 ttcgagtggt ggggtgaccc caattcgcgc gagacgtgcg ccgaaaatct gaggaatgcc
    10621 gaaccccccg cgggatacct tcttccttac tggatgggac ggtacttcgg cttcatttca
    10681 caggatttgt gaaaacgccc ctgttccagg cggacagacc gcgcgaaatc ggctattctt
    10741 ccggcattta atcagactcg gggatgactc gatggccagg attgcttttg ttcgtgccgc
    10801 gctgtcggcg gttgtcttgg tatggctctt tgtcgcgtcg ggatgcggcg gcggtgaaga
    10861 ttccggtgac ctgaccgggg atcagggctt cgtcgatgat gtcgtcggcg aactggcggc
    10921 cgtggacgtc gccagcgatc atcgcaccga cacgcccgtg gatgttgcgg atgaccccgg
    10981 cacgattgat gccatcgacc cggacgccat cgatgccgat tccgccgatt cgtcgggcga
    11041 cgatggcgat gtcgccggcg atgtggtcga tgaagacgtc atgccggatg tgccacgcag
    11101 ggacgccgag tgggatgatg tgccggttcc accgccctcg ttcccgattg tcagggacgg
    11161 ttccgccact cacgtcatcg tggttgccga cgaagccagc cagtccgaaa agaccgccgc
    11221 cgccgagatt cagtcaatca tcgtgcaggc gaccggggtg gaacttcaga tggccgcggt
    11281 gccgcccggc ggcgacgtcc cgatgattgt cgtcggttgc gggcttgctg cgagcggcct
    11341 tggcgtgacg gaagagccgg attccttcga cgaaggcgaa taccttatcc gggtggtcgg
    11401 gaattccgtc gtcatcgccg gcaacccggc ggccgggacg atgcacggcg ttcatgaatt
    11461 tctgaaggcg gccgcaggcg tccgatggac ggccccgggc gtcaccgtcg tcccgcaggc
    11521 ttcagaaatc gtgttccccg ttcaatacga ccgtcgctac aagtcgcctt tcaaatggcg
    11581 cacgacgaca tacgagtggc cgggcgccga ccgcgaattc tgggcccacc aggccgaaaa
    11641 cagtggatct tccggccccg acgccgcgtt tggcgcagag cactggcatg acggaatagc
    11701 ccattcgtat ttcagtttca tttcgccgga cgagttctgg gacacgcatc ccgagttctt
    11761 cagcgaaatc gggggcgtgc gcgtcaggga agagacgcag ttgtgcctta ccaatccgga
    11821 cgtgctcgat atcgtcaccg agcgcatgct ggcaaggatg gccgcgcatc ccgacgcgat
    11881 gcagcacaac ttcagccaga tggatcatta caaccagtgc cagtgcgaca agtgccgggc
    11941 catgaacgag ctttaccagt cagagggcgg cacgcagttc tggttcgtca acgaactggc
    12001 aaaaaggacg tctctggaat acccggacaa gcagatcggc acgctggcgt acatgtacac
    12061 cgaggagccc ccggtcggcc tggaacttca cccgaacgtc gccgtctggc tgtgtcacat
    12121 gtatccgtca tgcgacagcc atccgatccg gacttgtccc gataacgccg attacaaacg
    12181 tcgggcccag gcatgggccg aattgacgga tcatctgtac atctggcatt acatcgtcga
    12241 tttcatgcat tattacaatc ccttccccaa tttctgggcg ctcgccgaca acattcgctt
    12301 ctatcgcgac atcggggtcg agggtgtctt ccttcagggc atgggacacc aggggggtgg
    12361 gggcgaattc agcctgctgc gtccgtggtt cggcatgcag cttctatgga atccggatca
    12421 ggacgtcacg gccctgataa agcgtttcct gaaggattac tacgggccgg catggggccc
    12481 gatttgggac tggatcgtcc tgcttcaggc gaaggtggat gatgaagaca tccacatgca
    12541 cctttactcc aatccggcac agggctatct gcccgaggac atcgtcgtga cgggcgaacg
    12601 cctttttgcc gaggcggccg cgctggtcga aggcgacccg atacttgaag accgcgtcga
    12661 tgttgccagg atgcccttgc tgtacgcgcg cttctttccc cgcaacggat acacgatttc
    12721 cgacggctgg cttcggtgga acccgggaat ggcgacctgg gacgacgtca accttttcct
    12781 tcagatgatg aacgatcacg gcttcacgtc gtatcgcgag gcgcagggca gtccggatac
    12841 gatgacgatg ctgtacatga tgattggcgc cgatgccgaa gttaagacca tatcgaacgg
    12901 caagctttcc gttgacgtcg ttccgacgct tgcggggcgg gctctgcgca tcaccgatgt
    12961 cgcgtccggc cagaccgtca cggcctggga tcagcgaagg aacctgttct atccgttcca
    13021 gggggggctg gaggatcgtg tcggcgaggg attcaacttc atgggctgg
//
LOCUS       DAOPPA010000021        12869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5660_length_12869_cov_3.703918, whole genome shotgun sequence.
ACCESSION   DAOPPA010000021 DAOPPA010000000
VERSION     DAOPPA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_12869_cov_3.703918"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKH54_01310"
     CDS             complement(<1..1080)
                     /locus_tag="PKH54_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOC98552.1"
                     /translation="MNREDVLQLVPLGGFAAIFSGIIIGTRGLGPMVGLHLALVWVTA
                     WLIFRLTSGGRRLCHACTIRVAFLPGGWLPASVLLLVMLAGGVVLGAGAVPRDQILAS
                     LSGSEVRALVTVTEVGGRDANGVRARGLIEALSGAQGPVNGALRRPVSILCSHDGSSA
                     ARPGCRAIRAGLTIAVRGQAGYTDRVGSFIRLAGDAVPVAVPGSGSGLRGAIDSWRSI
                     KSFLCMDRLPGAASWLVPALVLGEGGGVPQRSRNALSVLGTAHLISVSGLHVVTVSGA
                     VGLLVFILIGLPASRLPVRLNAAKTASVSTVGCAWLVALIAGLPPPAVRAAAVATLWG
                     LARFSSRFPGATSTVCSAGMLEMLFA"
     gene            complement(1220..2791)
                     /locus_tag="PKH54_01315"
     CDS             complement(1220..2791)
                     /locus_tag="PKH54_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC98553.1"
                     /translation="MSGLSRLTMRHMARARIHLVHAALGLVVGVATLTFFLGLSQGVR
                     QKVLNRLYPVNQVEFQPEKVRLFGMGMEVPSRLDDAVLKAIRARGDVTAVFPKQRSRF
                     QARLWGGADILGTPVRVEAFFDGIQPGLVAAELRQAEAAVLGREDSDAACAGHSGCGV
                     GAVCREGRCARKTWWDQFVESGTPEYSCRLGSPGVQVSDDREALRGRVTGSLSGLRRE
                     GLPEACPQGQYCAARALDSADGVCARPIPVILSPFLLDVYNDVAAGALGMRRLSGLEV
                     ALGVEFAIMYGESYFMPDEPVGRRVVRRCRVVGFSPRAMDFGVTMPLQDVVDANAMLR
                     PESGPVEYSSVIVQAAANEDVPALVEDMKVLGLVLAPKSEAGRKAANVLTILTLIFAG
                     VSGIILLISAINISHTFLMLVTERRREIAIYRAVGASAMQIRVIVWLEAALLGLAGGA
                     VGCLAGWVFSLIADALASRLVSGVPGSPSSFFQFDLWMFALCLSCSILFSIVGAHGPS
                     RDAARTDPAGVLAQN"
     gene            complement(2788..3501)
                     /locus_tag="PKH54_01320"
     CDS             complement(2788..3501)
                     /locus_tag="PKH54_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC98554.1"
                     /translation="MDESVSDREPGGMPLPAVRTINLGKVYPDRALGHRSALAGVSLE
                     IGRGEWVSLMGRSGSGKTTLLNIIGGLDLDWTGQVEVLGRPMACLRDAEMSRFRRNSI
                     GFVFQAFNLLGHLNLLENVMLPSHFGNGSPDESREAAIASLEMVGLGTRVESFPWQLS
                     AGERQRVAIARAIVNRPELLLCDEPTGNLDAVTGQSIIDIFREINRNRSVTIIVATHE
                     QKMADASGRTIFLDSGRVA"
     gene            complement(3479..4933)
                     /locus_tag="PKH54_01325"
     CDS             complement(3479..4933)
                     /locus_tag="PKH54_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98555.1"
                     /translation="MKRLLLAVMAFSFVGCGSSGPDPRMLIPVDAEAWLAVRPAREVA
                     ERIGPIVDQYPEYGGALDFARSVIGIDYARPDRESGLDPARGTVFAWFRGGLLVILPV
                     VDQKLAVRRAALRMARFGFVQSAEQGGLIHFESSEHGHGCIVAKAGIAAVYFGPADSC
                     PLLAGIADQADDSRESQVRLAFDRVTSEMEMEGADLIFFVANSLYSKRLLRMAGFPEK
                     APVTYLASGVMGDLRGAVTIDRELVARIAAGAVGTPFRPVPAGGRGVEPGDAARIDVT
                     IGPSLRPLIDALVPMCGARCSRAGIDMMVGSWDGRLEVKVPASDGSASRGSGSGVLRA
                     LAAMTGKVTVTGVAGTRRPGRTILDFARRRLGQQLPEEDAGAADKPVAFTFDGHDVAV
                     GAGRDRLAVAIGPSALESVSRITVAGVPGDLPEWPTDGRILSARVNPEALLDLSGFGF
                     IQYLERLVNPVKGVAAEVFFDNGRILVDGRISIR"
     gene            5118..6560
                     /locus_tag="PKH54_01330"
     CDS             5118..6560
                     /locus_tag="PKH54_01330"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOC98556.1"
                     /translation="MKNAFSSLAAIVVLTAIIGRPDAGLASSEKPDLWTERPQPVFDS
                     SFNPGSGFAPLVKAVEPTVVNINTTMKMERGMPGGGSNDFWLRFFGGMQMPPAERQSL
                     GSGLIINSEGYILTNNHVVENATEIKVKTTKPQFELPARIIGRDERMDLALIKVDAPE
                     PLPVAVFGDSDGLEVGEWVVAIGSPYGLARTVTAGIVSAKDRVIGAGPYDDFIQTDAS
                     INPGNSGGPLFNARGLVVGINTAIHAAGQGIGFAVPISMAKKFVHDVMTKGHVSRGWL
                     GVGIQELTPEIAKGMGISLSRGVIISQVHENSPAAKAGFKVGDIVTAFGRVEVGQPSD
                     LVRAAGLTNPGTTVPVTVFRENRSVTLQVTIAEREDDEVAIVEGESPLLQGQTTMLGM
                     TVRELKPAEARRLGIREGYGVRVVSVDSAGPAAAAGLVQGDIVVEVNRTPVRNGESFT
                     KTLKKIRSGETVLLLTVRGSNYLYRVIRKP"
     gene            6580..7572
                     /locus_tag="PKH54_01335"
     CDS             6580..7572
                     /locus_tag="PKH54_01335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOC98557.1"
                     /translation="MTDNEIDSSPEEPEIPDTSDQPEVIDIRPGEVTSLALRPTGDDT
                     SHQVDLLYRYIRDIQRYPLLTPEEELDLTTKFVETGDPEIAYRLVSANLRLVIKIAME
                     YRTFTSQVLDLIQEGNIGLVKAVQHFDPVRGVRLSHYAQYWIRSYIIYYLLNNHRMVK
                     LGTTQAQRKVFFNLRKERQRLLNMGYDPTARMIATNLAVPESTVNEMMQRMDAPDLYL
                     DAPHGTDGKTTYMSSMAGDTDIEAEASAREISDRVREVIVAFGRLLESDRDVYIWENR
                     LLSDDPVTLQSVGDKFGVSRERARQIEERIKRKFKEFLQKELGEDFVQAQILSD"
     gene            complement(7589..8542)
                     /locus_tag="PKH54_01340"
     CDS             complement(7589..8542)
                     /locus_tag="PKH54_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC98558.1"
                     /translation="MMRVLITGGAGFIGSHLCERFLDDGHQVVCVDNLLTGSTANIEP
                     LFGRDGFVFIKLDCTEFLHIPGQLDAVLHFASPASPFDYLKYPIETLKVGSLGTHKLL
                     GLAREKKCRFLLASTSEVYGDPLVHPQPESYWGNVSSIGPRSVYDEAKRFAEAITMAY
                     HRHHGVDTRIVRIFNTYGPRMRMGDGRVIPAFLGGALRNEPLPVFGDGSQTRSFCYVS
                     DLVEGIVRLLDSHEHMPVNLGNPNEFTVMELAQTVASMFGVKLDVRNMPLPQDDPKVR
                     RPDISRARAILGWEPRVELKDGLTMTADYFRGVLERLQSGA"
     gene            complement(8634..10091)
                     /locus_tag="PKH54_01345"
     CDS             complement(8634..10091)
                     /locus_tag="PKH54_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98559.1"
                     /translation="MNMHGHGVRSLMPVALALAFMVLPAPVFPASADSEQAQVGAPVE
                     VDLDLARRIAGSVFGLDISGVEQDSSAFRFSVSGFSDSRRCPLSGLRNLSCESFEWIP
                     SRITIYRKARQAPVSGHLARNLVVMVDMESDSVGFAIGTLHGVLVTPSGGLSAGRPCA
                     FGWYLSRSGYSIGMSVRDVSGDGAADVVYSYGQVLPRGVSIAVRDVWSFPAMAATKFI
                     SSGEKLSGVIGSTFEGVPIHLSSDDRTELGRFVFLSGDSGRTAVPGSVPAGILAIERL
                     VLTPPVPVWEFQVIGDTGGGWLDFLAIQGSIRDDEGPVAEPGLLDRMDPESAEMLSGD
                     RCTMSDLPTDLSVQIRRALLDAEDTCRVASAGVPASPVAASAAAMLIRGFALAWKGWP
                     LLAASFFEAASVELLASGRALPAVAASWFAALGTVSAVEALCGEDAAGSCEAGGIDPL
                     HGLYLDSVARIAAGAMDGLRGVVDRMVEGLRKLPE"
     gene            complement(10088..12115)
                     /locus_tag="PKH54_01350"
     CDS             complement(10088..12115)
                     /locus_tag="PKH54_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98560.1"
                     /translation="MHFRPKSRFWPSIPGLIFAFVAGCGQGDDPGADIARDSFQDVHQ
                     ADSTTEVADLLQDDPGTDSEVTDPADSGIELDAVEDGITETVDVPGEDVVNAGAFSVM
                     DLRDAGDILAIWASAGGEVWAVGRGGLVLVRRNGQFVPGPRPPVNVDFHGVTGVDGAI
                     LVVGENGTVLKFVDGRWLDMECPVDVTLNAISCPVAGECFAVGDSGTIVHWLEDQWDV
                     QDSGVAWDLFDVHSSVNGGTWVVGAFGSLFELSGTDWLSSQIAGTRSSMKSIWRSGDG
                     TMFAVGTLGTIVMKRPGDLYWQQQLSNDGHDPQRDLFSVGGFDSDDVWVVGDSGVIIH
                     YDGVYWKTATVIGPATTLADFRSVAVGPGGTVVAAGLRSSIAGLDAELEGFVDVAAGP
                     VSDLRGVWVEKSSGADDDVAIFVGDDGLMLEYDRGRFSLLESGLDQAIRSVDGGIAVG
                     DGGLVLAIGATDSDGTRYVSRIPGFTVEDLVDVHASGDGWVIAGADGSVFRMDSAMSV
                     TFAGQVAVRLASVCRNEAGIWVAGANGTLSLLPSGNDEGQVFEDVVTFTRSFIRDLLP
                     LPGGRVLAAGDNGMMLVCDTIRCDTVYQEPASFLYGLGAINRGDGPEVYAVGWAGRVV
                     RFDGDSARRVESGTFKVFLAADGADSAGGRLFIAGSRGTAAIFTRNDGGSD"
     regulatory      12249..12421
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12529..>12869
                     /locus_tag="PKH54_01355"
     CDS             12529..>12869
                     /locus_tag="PKH54_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98561.1"
                     /translation="MFKFEGTLLMPVCVLALYAIACSGDDRQESDVIEDGEVANDIVD
                     DGQTADMPIDSTGGRPLEIGPHSGLPIAGEWFDDWDARHSITSSRWIQSSDFGTSTFE
                     IIEFDKENRSVV"
BASE COUNT         2847 a         4239 c         3652 g         2131 t
ORIGIN      
        1 cgcgaagagc atctcaagca ttcccgccga acagactgtc gatgtcgctc cggggaatcg
       61 ggaagaaaac cttgcaagcc cccacagcgt cgcgaccgcg gcggcgcgaa ccgccggagg
      121 cggcaacccc gcaatcaacg caaccagcca cgcgcagccc acggtcgaga cggaagctgt
      181 cttcgcggcg ttaagcctta caggcaatcg tgatgcgggc agaccgatca gaatgaagac
      241 cagaagtccg accgctccgg aaacggtaac cacatgaagc ccggaaacgg atatcagatg
      301 tgcggtgcca agtaccgaca gagcgttccg ggaacgctgg ggaaccccgc cgccctcccc
      361 cagcaccagg gccggcacca gccatgatgc cgcgccgggt agcctgtcca tgcaaaggaa
      421 cgacttgatt gaacgccacg aatcgattgc cccgcgcagt ccggaaccgg atcccggaac
      481 cgccaccgga accgcgtcgc cggccagcct gatgaacgac ccgacgcgat cggtgtatcc
      541 ggcctggccg cgaaccgcga tggtcagtcc ggcccttatc gcacggcagc ctggccgcgc
      601 tgcgctggaa ccgtcatgcg agcacaggat ggataccgga cgacgcaacg ccccgttgac
      661 cggcccctgt gctcccgaca gcgcctcaat caagccccgc gcccgcaccc cattcgcatc
      721 cctcccgcca acttccgtga cagtgaccag cgccctgact tcactgccgg acagcgacgc
      781 caggatctga tcgcgcggca cagccccggc tccaagaacc acgccgccgg ccagcatgac
      841 caacaggaga accgatgccg gcagccaccc gccaggaagg aacgccacgc ggatggtgca
      901 ggcatgacac aggcgccgac cgccactcgt caggcgaaag atcagccagg cggtaaccca
      961 gaccaacgcc agatgcaatc cgaccatcgg ccccagcccc cgagtcccga tgatgatgcc
     1021 ggaaaagatt gcggcgaagc cgccaagtgg caccagttgc agtacatctt cccgattcat
     1081 gcacgttatg tcggcattcg gggcgaatcg tttccgtcaa cacagcaagc cgcaactgct
     1141 tggattttaa gtgaaaccaa aaaggatcca gatgaagcca aatgggatcc agacgattgg
     1201 ttcgcgcgga tcaggccgat cagttctggg caagaacgcc ggcgggatcg gttcgggcgg
     1261 catcacggga cgggccatgc gccccgacga ttgaaaacag gattgaacac gaaaggcaca
     1321 gtgcgaacat ccacaagtcg aactggaaga agctagacgg actccccgga accccggata
     1381 caagccttga cgccagtgca tccgcaatca ggctgaatac ccacccggca aggcatccga
     1441 cggcgccgcc ggccagcccg agaagtgccg cctcgagcca gacgattact cgtatctgca
     1501 tggccgacgc tccaacagcc cgataaatgg caatctccct acggcgctcg gtgacaagca
     1561 tcaggaacgt gtggctgatg ttgatggcgg aaatcagcag gatgataccg ctgacgcccg
     1621 caaatataag ggtaagtatc gtgaggacgt tcgcggcctt tcgaccggct tcgctttttg
     1681 gcgcaagaac gagcccgaga accttcatat cttcaaccag ggctggaacg tcctcgtttg
     1741 cagcggcctg gacgatgacg ctggaatact cgacggggcc cgattccggc cgcagcatcg
     1801 catttgcatc gacaacgtcc tgaagcggca tggtcacccc gaagtccatc gccctgggcg
     1861 agaacccgac gacgcggcac cgccgcacca cccgccttcc aaccggctcg tcaggcatga
     1921 agtaggattc cccgtacatg atcgcgaact cgacccccag ggccacttcc agccccgaaa
     1981 gcctgcgcat tccaagggcg ccggcggcca cgtcgttata tacgtccagc aggaacgggg
     2041 acaggattac cggtatcggc cgggcacaca cgccatcggc cgaatccagg gcgcgggcgg
     2101 cgcagtactg accctgggga catgcttccg gcagtccttc ccgccgcaac ccggacaatg
     2161 acccggtgac gcgcccacga agggcttcgc gatcgtctga cacctgaacc ccgggtgaac
     2221 ccagccggca ggaatactcc ggcgtgcccg actcgacgaa ctgatcccac cacgtcttcc
     2281 tggcgcagcg gccttcacgg cagacggcac caaccccgca accggaatgc ccggcgcagg
     2341 ctgcatcgct gtcttcacgt ccaagaaccg ccgcttccgc ctgccgcagt tcggcagcga
     2401 caagccctgg ctgaattccg tcgaagaagg cctctaccct gacaggggtg ccaagtatgt
     2461 ctgcgccacc ccatagtctc gcctggaacc tggatcgctg cttggggaat acggccgtga
     2521 cgtcccctct tgcccggatg gccttaagaa ccgcatcgtc aagccttgac gggacttcca
     2581 ttcccatgcc gaaaaggcgc accttctcgg gctggaactc gacctggttg acgggataaa
     2641 gacggttgag caccttctgc ctgacaccct gggaaagccc caggaaaaat gtcagcgtcg
     2701 ctaccccgac caccagtccg agcgccgcat ggacgagatg gatcctggcc ctggccatgt
     2761 gacgcatcgt aagacgggac agaccgctca tgcgaccctc ccggaatcga ggaatatcgt
     2821 ccgccccgag gcatccgcca tcttctgctc gtgtgtcgcg acaatgatgg tcacggatcg
     2881 gttgcggttg atttcccgga agatatcgat gatcgactga ccggtcactg cgtccaggtt
     2941 accggtcggc tcgtcgcata gaagcagttc cggacggttg acgattgccc gcgcaatcgc
     3001 cacgcgctgc cgctcgcccg ccgaaagctg ccagggaaag gattcgacgc gcgtaccaag
     3061 ccccaccatt tccagtgaag cgatggcggc ttccctggat tcgtcggggc tgccgttgcc
     3121 gaaatgggac ggcagcatga cgttttcaag caggttaaga tggcccagca ggttgaaagc
     3181 ctggaatacg aaaccgatcg agttccttcg gaagcgcgac atttcggcgt cccgaaggca
     3241 ggccatgggt cgtccaagta cctccacctg cccggtccag tccagatcca gcccgccaat
     3301 gatgttcaga agcgtggtct tgcccgaccc ggaacgcccc atcagcgaaa cccactcgcc
     3361 gcgcccgatc tccagcgaca ccccggcaag cgccgatcga tggccgagcg cgcggtcggg
     3421 atacaccttc ccgagattga tcgtcctgac cgccggaagc ggcatgccgc caggctccct
     3481 atctgatact gattcgtcca tcgaccagga tccgcccgtt gtcgaagaag acctcggctg
     3541 ccacgccctt gaccggattg acgagccgct cgagatactg gatgaaaccg aacccgctca
     3601 agtcgagcag ggcctccgga ttgaccctcg ccgacaagat ccttccgtcg gtcggccatt
     3661 ccggcagatc tccgggaacg ccggctacgg ttatcctgga aacagattca agcgcgctcg
     3721 gaccgattgc taccgccagg cgatcgcggc cggccccgac cgccacatca tgaccgtcga
     3781 aggtgaaagc cacaggtttg tcggctgcgc cggcgtcctc ttcgggaagc tgctgcccca
     3841 gccggcgcct tgcaaaatcc agaatggtcc ggccaggtcg gcgcgtcccc gcgaccccgg
     3901 tcaccgtgac ctttcccgtc atcgccgcca gcgcccgaag caccccgctt ccacttccgc
     3961 ggcttgccga accgtccgaa gccggaacct tgacttcgag ccttccgtcc caggagccca
     4021 ccatcatatc gatcccggcc ctggaacagc gggcgccaca catcgggaca agtgcgtcaa
     4081 tcagtggcct cagcgacggc ccaatcgtga cgtcgatgcg cgccgcatcg cccggttcaa
     4141 cgcctcggcc cccggccgga acgggcctga acggcgtccc gacagccccg gcagcaatcc
     4201 ttgccaccaa ttccctgtcg attgtcacag ccccgcgcaa gtcgcccatc acgcccgaag
     4261 ccaggtatgt caccggcgcc ttttccggga accctgccat gcgaagcaat ctctttgaat
     4321 acagggagtt tgcgacgaag aagatcaggt cggcgccttc catttccatt tcggaagtca
     4381 ccctgtcgaa cgcaagcctg acctgcgatt cccttgaatc atccgcctgg tcggcaatcc
     4441 cggcgagcag gggacatgaa tcggccggac cgaaatagac agccgcgatg ccggcctttg
     4501 ccacgatgca tccgtggccg tgttcggacg actcgaagtg gatcagcccg ccctgttcag
     4561 cggactggac aaaaccgaac ctggccatgc gcagggccgc acgccttacg gcaagcttct
     4621 gatccaccac gggcaggatg acgagcaggc cgcccctgaa ccaggcgaac accgttcccc
     4681 gcgccgggtc aagaccgctt tccctgtccg gtcgcgcgta atcgattccg atgactgaac
     4741 gcgcgaaatc aagcgccccg ccatattcag gatactgatc gacaatcggc ccgattcttt
     4801 cggcaacctc gcgggccggc cgcacggcaa gccaggcctc ggcatccacg ggaatcagca
     4861 ttcgcggatc cggtccagat gatccgcagc cgacaaagga aaacgccatg acggcaagca
     4921 agagcctttt cacgatcccc ccgccagaat gcaacaggac acgcaccgaa cctgctgccg
     4981 atgttaccat acaccccgcc cccgttacgc attgacggga acaaactgat atttacattt
     5041 gttttcaggt cgccggccgg aagcggccga ctgacccgat atgactgatt gggatggcca
     5101 tgataagggg ttatgtaatg aagaatgcat tttcatcgct tgccgcaatt gtggtgctta
     5161 cggcaatcat tggccggcca gacgccggcc ttgcgtcatc cgaaaaaccc gatctctgga
     5221 ccgaaaggcc ccagccggtc tttgattcgt cgttcaatcc cggatcaggc tttgctcccc
     5281 tggtgaaggc ggtcgaacct accgtagtca acatcaacac gacaatgaag atggaacgcg
     5341 gcatgcccgg cgggggatcg aacgacttct ggctgcggtt cttcggcggc atgcagatgc
     5401 cccccgcgga aaggcagtcg ctgggatccg ggcttatcat caattcggaa ggctatatcc
     5461 tcacaaacaa tcacgtggtc gaaaacgcca ctgaaatcaa ggtcaagacc acaaagcccc
     5521 agttcgaact gcccgcaagg ataatcggcc gggacgaaag gatggatctg gcccttatca
     5581 aggtggacgc cccggaacca cttccggtgg cggttttcgg cgactccgac ggcctcgagg
     5641 tcggggaatg ggtggttgcg ataggcagtc cgtacgggct ggcgcgcacc gtcacggccg
     5701 gaatagtgtc ggccaaggat cgcgtaatcg gggcaggtcc gtacgacgat ttcatccaga
     5761 cagacgcgtc gatcaaccct ggaaactccg ggggaccgct tttcaacgcg cgcggactgg
     5821 tggtcggcat caatacggcg attcacgcgg ccggccaggg tatcgggttc gcggttccga
     5881 tatcgatggc aaagaagttc gttcacgacg taatgacgaa aggccacgta agccgaggct
     5941 ggcttggcgt tggaatccag gaactcacac cggaaatcgc aaaaggaatg ggcatttccc
     6001 tttccagggg cgtaatcatt tcgcaggttc atgagaacag cccggccgcg aaggctggct
     6061 tcaaggtggg cgacattgtg acggccttcg gtcgggtcga ggtcggccag ccttcggacc
     6121 tggttcgcgc ggccggcctg accaatccgg ggaccaccgt tcctgtcacg gttttccggg
     6181 aaaaccgcag cgtcacgctg caagtcacca tcgcggaacg cgaggacgat gaagtcgcca
     6241 tcgtggaagg ggaatccccc cttcttcagg gacagaccac aatgctcggg atgacggtca
     6301 gggagttgaa gccggccgag gcgcgccggc ttggcatccg ggaaggctac ggcgtcaggg
     6361 tcgtttcggt cgattcggcc ggacctgccg cggccgcggg actcgtgcag ggcgacatcg
     6421 ttgtcgaagt caaccggact ccggtgcgaa acggcgaaag ttttacaaaa actctgaaaa
     6481 agatacgatc cggtgaaacc gtcctcctgc tgacggttcg agggtccaac tacctttaca
     6541 gggtgatcag gaagccctga ccggacggat ggaagaaacg tgaccgacaa cgaaatcgac
     6601 agcagcccgg aagaacccga aattccggac acatcggatc agccggaagt catcgacatc
     6661 aggcctgggg aagtgacctc gctggccttg cgtccgacgg gggacgatac atcccatcag
     6721 gttgatcttc tttacaggta catcagggac attcaacgat atcctttact tacccctgaa
     6781 gaggaactgg atcttacaac gaaattcgtc gagaccggcg acccggaaat cgcctacagg
     6841 cttgtttcgg caaacctgcg tctggtgatc aagatcgcca tggaataccg gacgttcacg
     6901 tcccaggtac tcgacctgat ccaggaagga aacatcgggc tggtcaaggc cgtccagcac
     6961 ttcgatcccg tccgcggcgt tcggctgtcc cattatgccc agtactggat tcgctcgtac
     7021 atcatctact acctgctgaa caaccaccgg atggtcaagc ttggaacgac acaggcccag
     7081 cgaaaggtct tcttcaatct tcgaaaggaa cgccagcggc ttctgaacat gggatacgac
     7141 cccacggcga ggatgatcgc gacgaacctg gccgtccccg aaagtaccgt gaacgagatg
     7201 atgcagcgca tggatgcgcc ggatctgtac cttgacgcgc cccacgggac cgacggaaag
     7261 acgacgtaca tgtcctcgat ggccggcgac accgatatcg aggcggaagc gtccgctcgc
     7321 gagatttccg accgcgtgcg ggaagtcatt gtcgcgttcg gacgccttct ggaaagcgat
     7381 cgcgacgttt acatctggga aaacaggctg ctctcggatg atcccgtgac gcttcagtcc
     7441 gtcggagaca agttcggcgt cagccgcgag cgagccaggc agatcgagga acgtatcaag
     7501 cgcaaattca aggaattcct gcagaaggaa cttggggagg atttcgttca ggcccagatc
     7561 ctttcggact gaccctggcg gccgggcgtc acgcacctga ttgcagtctt tcaagaaccc
     7621 cgcggaaata atcggcggtc atcgtcaggc cgtccttcaa ctccaccctg ggctcccagc
     7681 cgaggatggc tcgcgccctg gagatatccg gacgccttac cttgggatcg tcctgcggca
     7741 gcggcatgtt gcgcacgtcc agtttcacgc cgaacatcga cgccaccgtc tgcgcaagct
     7801 ccatcaccgt gaactcgttt gggttcccaa gattgacggg catgtgttca tgggaatcaa
     7861 gcagcctgac aatcccttcg acaagatcgg acacatagca gaacgacctt gtctgggaac
     7921 cgtccccgaa caccggaagg ggctcattcc tgagcgcccc gccaagaaaa gccggaatga
     7981 cgcggccatc gcccatgcgc atcctggggc cataggtgtt gaaaatccgg actatcctgg
     8041 tatccacgcc atgatgccga tggtaggcca tcgtgatggc ctccgcgaac ctcttggcct
     8101 cgtcatacac gctgcgcggc ccgatgctgc tgacgttgcc ccaataactt tccggctgtg
     8161 gatgaaccag cggatcccca tagacctcgc ttgtcgaggc gagcaggaac ctgcatttct
     8221 tttcgcgggc caggcccagc aacttgtggg ttccaagcga tccgaccttc agcgtctcga
     8281 ttggatactt cagatagtcg aacgggctcg cgggcgaggc gaaatgcaga acggcgtcga
     8341 gctggccggg aatatgcagg aactcggtgc aatccagttt gatgaacacg aaaccgtcgc
     8401 gaccgaaaag cggctcgatg ttcgctgtgc tccccgtaag aaggttatcg acgcagacaa
     8461 cctgatggcc gtcatcgaga aaacgctcgc acagatggga acctatgaaa ccggcaccgc
     8521 ctgtaatcag taccctcatc attcctccgg aaaacggtcg gcatccgggg cccgcgtgcc
     8581 gaccagggca tcaaaacgac gcgaaggatg cgccaaagcg ctgttgaagt caatcattct
     8641 ggcaacttcc gcaggccttc gaccatccga tccacgacac cccgaaggcc gtccatcgcg
     8701 cccgccgcaa tgcgcgcaac cgaatcaagg tataaaccgt gcaggggatc gattccgccg
     8761 gcttcacaac ttcccgcagc atcctcgcca cacagcgcct cgaccgcgga aaccgttccc
     8821 agggcggcaa accacgatgc ggcgacagcc ggcagcgccc ggcccgacgc cagcagttcg
     8881 accgaggccg cctcgaagaa cgaggccgcc agaagcggcc atcccttcca cgcaagcgcg
     8941 aacccgcgaa tcagcatggc agccgcgctt gccgcgaccg gactggccgg aacccctgcg
     9001 gacgcaaccc tgcaggtatc ctcggcgtcg agaagggccc gccttatctg aacggaaagg
     9061 tcggtcggga gatccgacat ggtgcagcga tccccggaca acatttccgc cgactccgga
     9121 tccatccgat cgagcagtcc cggctccgcg accggccctt cgtcgtcgcg gatgctgccc
     9181 tgtatcgcga gaaaatcgag ccacccacct ccagtatccc cgatgacctg gaattcccag
     9241 accggaaccg gcggtgtcag aaccaggcgc tcgatcgcca ggatccctgc cggaacgctt
     9301 cccggaacgg ccgtccgtcc cgagtcgccc gaaagaaata cgaagcggcc aagctccgtt
     9361 ctgtcatccg aggaaaggtg gattggaacc ccctcgaacg tactgccgat aacgcccgac
     9421 agcttctccc ccgaagagat gaacttcgtg gccgccattg ccgggaaaga ccagacgtcc
     9481 cgcaccgcta tcgacacgcc gcgtggaagc acctggccgt atgaatagac cacgtccgca
     9541 gccccgtcac ccgacacgtc cctcacggac attccaatgg aatacccgga acggctcagg
     9601 taccagccga acgcgcaggg gcggccggcc gaaagtcccc ccgaaggcgt gacgagaacg
     9661 ccatgcagtg ttccgatggc gaaacctacg gaatccgact ccatgtcaac catcacgacc
     9721 aggttccttg ccagatgacc ggatacaggg gcctgacgcg ccttgcggta aatcgtgatg
     9781 cgtgacggga tccactcgaa agactcgcag gaaaggttcc gaagcccgct cagcggacat
     9841 cgccttgaat cggaaaaacc ggacacgctg aacctgaatg cggacgagtc ctgttcaacc
     9901 cccgatatgt caagcccgaa caccgaaccg gcaatccgcc ttgcgagatc cagatcgact
     9961 tcaaccggcg ctccgacctg ggcctgctcc gaatcggccg acgcgggaaa gaccggcgcc
    10021 ggaagtacca tgaatgccag tgccagcgcg accggcatca acgagcggac tccatgcccg
    10081 tgcatattca atcagatccc ccgtcattcc gggtgaagat ggccgccgtt ccgcgggaac
    10141 cagcgatgaa aagcctgcca cccgccgaat cggccccatc cgccgcgaga aacaccttga
    10201 aggtacctga ctcgacgcgc ctggccgaat ccccgtcaaa ccgcacgacc cggccagccc
    10261 agcccaccgc atagacttca ggaccgtcac cgcgattaat cgcgcccaga ccgtaaagaa
    10321 acgatgccgg ctcctgatag accgtatcgc accggatcgt gtcgcaaacc agcatcatgc
    10381 cgttgtcgcc ggccgccaga acgcgaccgc ccggcagggg cagcagatcc ctgatgaaag
    10441 acctcgtgaa cgtgacgacg tcctcgaaaa cctgtccttc gtcgtttccg gaaggcagca
    10501 gcgaaagggt accgttcgct ccggccaccc agattcccgc ttcgttacgg cacaccgaag
    10561 caagcctgac cgcaacctgg ccggcgaacg tcaccgacat tgctgaatcc atcctgaaca
    10621 ccgagccgtc ggcaccggca atgacccagc cgtcgccgga cgcatgcacg tcaaccaaat
    10681 cttcaacggt gaaaccgggg attcgggaaa cgtatcgggt accgtcggaa tcggtcgccc
    10741 cgattgcaag caccagaccg ccgtcgccga ccgcgatgcc gccgtcaacg gatcggatgg
    10801 cctgatcaag tccgctttcc agcaggctga accggccacg gtcgtattca agcatcagcc
    10861 cgtcgtcccc gacaaaaatc gccacgtcat catcagcgcc cgacgacttt tcaacccaga
    10921 cgccgcgaag atccgaaaca ggtccggccg cgacgtccac aaagccttcg agttcagcgt
    10981 cgagaccggc gattgaggat cgcagcccgg cggccacgac ggtgccgccg ggaccgacag
    11041 cgaccgacct gaagtcggcc agggtcgtag ccggcccgat aacggtggca gtcttccagt
    11101 aaaccccgtc gtaatgaatg atcacgcccg aatcgccgac aacccagaca tcatcgctgt
    11161 cgaacccgcc gaccgaaaac agatcgcgct gggggtcatg cccatcgttt gaaagctgct
    11221 gctgccagta aagatcccct ggccgcttca tgactattgt tcccagggtc ccgaccgcaa
    11281 acatggttcc gtcacccgac ctccagatcg acttcatgga ggatctggtg ccggcgatct
    11341 ggctggacag ccagtcggtt ccggaaagct cgaaaaggga tccgaaggcg ccgacaaccc
    11401 acgtaccccc gttcacggac gagtgcacgt cgaaaagatc ccatgcgacg cctgaatcct
    11461 ggacatccca ctggtcttca agccagtgga ctattgtgcc ggagtcgccc accgcgaaac
    11521 actcccccgc gacaggacag gatattgcgt tcaaggtgac gtcaacgggg cactccatgt
    11581 ccagccatcg tccgtccacg aacttcagta cggttccatt ctccccgacg accaggatgg
    11641 cgccatcaac acctgtaacg ccatgaaaat caacattaac aggaggtctc gggccgggca
    11701 cgaactgacc gttccttcgt accagcacaa gcccgccgcg tcctacggcc cagacctcac
    11761 cgccggccga ggcccatatg gccaggatat cgcctgcgtc gcgaagatcc atgacggaaa
    11821 aagcgcccgc gttcaccacg tcctcgccgg gcacatcgac ggtctctgta atgccgtcct
    11881 caacggcatc caactcgata cccgaatccg ccggatcggt cacttcggaa tcggttccgg
    11941 gatcatcctg caacaagtcg gcgacctccg tcgtcgaatc agcctgatgc acgtcctgaa
    12001 aggaatcgcg tgcgatgtcg gcgcctgggt cgtcgccctg cccgcagccc gcgacaaacg
    12061 cgaagatcag ccctggaatc gacggccaga acctgctttt gggtcggaaa tgcatcacat
    12121 accccgacgg atcgcttacg acccggaata ataccagatg accggacaaa atcttcagaa
    12181 tccactatcg atatcgaaca cgcgcaagtt tcgcgacggc gatcctggcg gttgactggc
    12241 gccttcattc gggataacaa tgccccgggg acgcgatgca ggaaaccggt gagatgccgg
    12301 tactgccgcc gcaactgtaa ccgggaacga aaccggcaaa ccacgggcac ctggtgcctg
    12361 gaagggtcgg tggaggccat acccggaagt caggacactg accgcgtcca tttggcacca
    12421 gtcgtttcga gcggaaacgg acgggccctg cctaaccggc ctcttatcca cacacaggtc
    12481 gggggatcag gcccctgttt ccattcccgg aagggaaatg gaggaaccat gttcaagttc
    12541 gagggaaccc tgctcatgcc agtctgcgtg ctggccttgt atgcgatcgc atgctccggg
    12601 gatgaccgcc aggaatccga cgttattgag gacggcgagg tcgcgaacga catagtcgac
    12661 gacgggcaga ccgcggacat gccgattgat tcgacgggcg gccggcctct tgaaatcggc
    12721 ccgcattccg gtctgccgat cgccggcgaa tggttcgacg actgggatgc ccgccactcg
    12781 attacttcct caagatggat tcaatcctcg gatttcggga catccacctt cgaaatcatc
    12841 gagttcgaca aggagaatcg ctccgttgt
//
LOCUS       DAOPPA010000022        12806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5688_length_12806_cov_3.783939, whole genome shotgun sequence.
ACCESSION   DAOPPA010000022 DAOPPA010000000
VERSION     DAOPPA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_12806_cov_3.783939"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PKH54_01360"
     CDS             complement(<1..1386)
                     /locus_tag="PKH54_01360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOC98562.1"
                     /translation="MEPLAKGYEHREIEGRWYDVWNRAGYFRAADVSDKPPYCIVIPP
                     PNVTGQLHMGHALTLTIQDILIRWKRMSGFNTLWLPGTDHAGIATQMVVERELKATEG
                     LSRFDLGREKFIERVWQVKHRHHDRIVEQMAALGVSVDWERERFTLDDGLSRAVRVVF
                     TRLYEEGLIYRAQRMVNWSPGIQTVLSDLEVEEKELKSHMWHIAYPVTGTSESLVVAT
                     TRPETMFGDTAVAVHPKDPRHAHLIGKTVDLPLTGKKIPIIGDDQAVDMEFGTGAVKI
                     TPAHDFNDFETGKRHNLPVLSILDKLGNLNDNVPEKYRGMERFAARKLVEADLAEAGL
                     LVKVEDYAHRVGHCQRSGVIVEPMVSMQWFVRAEPLARPAIDAVRDGRTQFVPGSWEK
                     TYMNWMENIRDWCISRQLWWGHQIPVWYCQEPGCDGMYVGETDPGPDTVCPKCGSRRL
                     VQDEDVLDTWFS"
     gene            1570..>1775
                     /locus_tag="PKH54_01365"
     CDS             1570..>1775
                     /locus_tag="PKH54_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC98563.1"
                     /translation="MSSVNLTLTGPSDMAASLASRIRDLRLLRGWTQATLSRRAGVTA
                     SSYRRFETTGHASLELVLKVAHALS"
     assembly_gap    1776..1785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1882..3135
                     /locus_tag="PKH54_01370"
     CDS             1882..3135
                     /locus_tag="PKH54_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOC98564.1"
                     /translation="MKKLEVRFTRSPGDSIPVGTLAWDRGRTYFEYASDFLATGLNIS
                     PFRLPFEPGLFEHRDHEFGPLPGVFDDSLPDGWGLLLMDHHIRSLGLNPAGVSPLDRL
                     AWLGTGTMGALTYHPPAGRDSGDINIFVLHELARQSQEILAGLAVDVLPQLLRAGVSP
                     GGARPKVLVGYNPATSEIISGEGDLPEGSEHWIVKFFAREDQPDTGAVEYAYSLMARG
                     AGIVMPPTRLFKTAEGDRFFGIRRFDRDGNRRCHVHTFGNLIHANFRIPSSDYTDLLK
                     ATSLLTRNHQEVLRAFRYMVFNVLTHNRDDHVKNFAFILDDRIAGTGSPQGGDWSLSP
                     AYDLLFTHGPGGEHSMTVAGEGRSPTRQHMLRVAERADLSTREADAIIVEVQDAVARW
                     PDHASAAGVGTATTEAIARFLTRLP"
     gene            3205..5376
                     /locus_tag="PKH54_01375"
     CDS             3205..5376
                     /locus_tag="PKH54_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98565.1"
                     /translation="MVGTDDRIAAIGGMPSEEDIVLFGPGCQMGCTFCPHSGGRAGHR
                     METVLAPDFRLPLAHRVTILAGDVVRKEVAPLVSALRSQGSVEVFAYAHPGTWDLDAL
                     DELTSAGLTGIQLMLPAARKAELAALTGGKGSLGLVARLIDHANRRGLRVAAEIPVVE
                     SSATLIDDTVQRFLKITALPDRVALRFVAEFHPVRGPVPWDHSIATPHVAAASALCRG
                     RQVPLILAHPEAPPPCVMDVPDASAAMYPGLSMYGLPRGAPRPFAACGSCAAAAVCHA
                     EGRFLGGAEPVLTAPLALTGTAGSDAPGPVAGIRPERSRYMASEGTDRRKGLDQPDTP
                     GPNASTIWRRQADLNLLRVELERRRIACRYPWEQLEAHDIRGVVTPCAGGWPLESTVN
                     DCVSWHGGSLLTAWNSPGMQNTRRAVAAGRAHDTCKRDCPAFHGGPMGAVPSMSAPTT
                     GVMFDNIIDNVREMLDGATVLQSRPQSISFSPTMRCTNRCRMCDIHEVMEAMGRPAEI
                     REMPDSLFDELLELLPTTRMLALTGGEPLVSKRMRDLLKRFSAERYPDGAVTITTNGQ
                     LLRENILRDLSGTRIRQFYVSLNAADDAMYEHVSGIPGGFTRVRNHLALLMDMAPRMA
                     GRPGVIVSFVVMRSNFTQLPEFLDLALGLKTGIRLLPIERDRMGESIFTDEPTLAAVH
                     AMLTGTVMPSIERLPWGYRAEIARLFSIIDGRLSRRDFSPL"
     gene            5476..6126
                     /locus_tag="PKH54_01380"
     CDS             5476..6126
                     /locus_tag="PKH54_01380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOC98566.1"
                     /translation="MPAQLVLASASPRRLELLEKVGFSVKAMPAIIDESQHLDEMPDT
                     FVKRVARAKVLTVVDRIQQTLYTDPEQARIQRGLRRDVPLRWVLGADTVVVIDNDIIG
                     KPTDHAHAVEILTRLQGREHYVLTGFCLYDMQKSKEGLQAVQTNVRMKEMSVKEIERY
                     LTVGESMDKAGAYAIQGVGMYLIDSLVGSYTNVVGLPVCQVVEMMDEMNARDILPY"
     gene            6177..6887
                     /locus_tag="PKH54_01385"
     CDS             6177..6887
                     /locus_tag="PKH54_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOC98567.1"
                     /translation="MTREESIRSGLNEVRDGIALACGQSGRSVDEITLIAVSKFMSVQ
                     DIETAFNAAQLHFGENYAQELLAKASSPQLAGKPIRWHFQGGLQSRKVRDIIPVASTI
                     QSVDRDSVIDELARRTDPAAPVDVFLEVNTGGEAQKSGAAPANAERLCRRLIQIPGVR
                     LKGLMTIPPFLEDPEASRPYFVALRRLRDELRARLGASGDVLAGLSMGMTSDYKVAIQ
                     EGATVVRVGTAIFGARTP"
     gene            6907..8961
                     /locus_tag="PKH54_01390"
     CDS             6907..8961
                     /locus_tag="PKH54_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98568.1"
                     /translation="MKVERRFAAGTVEILVLVFFFGLLFLYSFHPNWDIDIFWHLETG
                     EWISNNHRLPDTDIFSSIDKTRPWMTFQWAYEVITYQVNARLGFKAVRLMHALLYMGA
                     FALLYATYRRRLSSRSVALALLVTTLVLAEDRFRVRPEAFNFFFTAIVLPILLDCAKP
                     GAPRPRIRTIVLIAAVSLLWANIHAGGALTLLVGSGAMLGGTLLGLVLNRNGESVIRL
                     KYNAWVFGTAAFLMAISPNFIRGNIHAFTMVNHSRLLIPEWHPPAAYFMPSMGGQLTV
                     HTFLCGTIPYLVLGWAMLAFGLALTRAIGSRRDNDPDRREKPFGILLTALFFAVIAAQ
                     SSRFVYTSSFAIFLLLLSKPRMLGQIASNPSWRVIFMLVAGFLTLASFQYSVAGKKDG
                     LKGQFRMLSLDNEPDRFPEKASDAIAAMGLEGRIFHFTAWGGYLLYRHFPECAVFTDG
                     RGNFTLDERKVLIDTHRPWGREDALEEAWSKYPFEILLLPRPVFPMLTWDRTKWMLIY
                     RDDISEAFLRVTPENQTNIQRAMAWWKVVGISSTSDPVAFQEDYRRVRGLIDIRDPRL
                     DAKLTNAAQQAHTDSLANRVSGLHDGALILFGVGLHEKATKFFRMILDEGIRHSTAAL
                     YLAWCQYLGNREDLARETLMKNFSSPDILGMPDRGPLRQTGLRILDELGARVGLRKAA
                     PQ"
     gene            9057..10031
                     /locus_tag="PKH54_01395"
     CDS             9057..10031
                     /locus_tag="PKH54_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98569.1"
                     /translation="MSKILDTVLLLALPASGKSEVRTWLASVPPEKCVSDFHIGETVQ
                     LDDYPYVHFMRCIDDAMRAAGHAGPFFHANDRPFKDPRDWCTLIELLNEDYAALKGCI
                     RFTVKDPVAYLFERIDSAALRAGAPVRLGVLPADVVDAVRPALEAEAARIVTDLEAGK
                     PDSLEGRTIVIEFARGGAHGSTMPLFEPFGYRASLKQLSPAILKNAGILYIAVTPEES
                     RRKNYERTDPNDPGSILNHGVPIDVMMGDYGCDDMAWLLSRSDVPGTVRVERAGQVFH
                     VPLSIFDNNGDRTTFVRRGQANWKKEEVEYLRDGLKAAFDHLAGSDRF"
     gene            complement(10028..10813)
                     /locus_tag="PKH54_01400"
     CDS             complement(10028..10813)
                     /locus_tag="PKH54_01400"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC98570.1"
                     /translation="MGSPENLPLFKVTVRNSLGGGTSEYAFRSGRFIIGRADSCDIVL
                     DSERVSRTHAAFVVHDGGLLIQDLGSANGVLVNDVRIVGTREIRIDDLIRIGEFVLSV
                     SGARLDDGTYLRLKGTSAGFEGQVLSFAKSPALVGRAEDADVAVVHPSISRRHARVVR
                     RHDKTVVINDLGSSNGVEVNGVPVKVARLAQGDVLRLGTVELVVELPNEATLSQMPSP
                     IMFHPLVLWAEANRWKIWVVAILVAAVAVTIAGWKIGGIAGGR"
     gene            complement(10855..>12806)
                     /locus_tag="PKH54_01405"
     CDS             complement(10855..>12806)
                     /locus_tag="PKH54_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC98571.1"
                     /translation="IVLPKPNVSKRHAVIRLADGHIVFEDLKSTNGSYVNGRRISSPR
                     ELVVEDRVYIGDYVLQVTQVEPDAGQSVEVQPEPEQAEARGATIAMPAFDIASPSAAA
                     DVASDLMEMPPLPGDSVLDQLPDMPQEPSAATEFEVEVEIEEPVAPIQPQVEPVQPVA
                     PPPKVVEVPKPAPAPRPAPAPAPAPAPAPAPAAAPAPAAAPVAPAAPVPAPAPVPAAA
                     VQPEPIPVAVDPATAVSGLARRLGYSGGKDGHAALRALSELAGREVFGSIPPEKADFT
                     DAEWQKLSDGVMRLVDRLRRENQIPADVDPIDITQKVLFEFAGLGPLEELLSNPAIKA
                     IIVGGLEGIQVIEDAGSRTINEVFSNFNTFNRVAVKLCSLAGIGSQALSQPLVEGRLP
                     DGSTLQILGAPFIDQGRIIVIERPMAAAMTELQLKESAGLSEKTIQALKAAVEARGNI
                     IVCGPPYTTRCVITNSLAMMVGAESRVLALGGRRDIDINLPNLVSIRRNALAQASSGA
                     AQIISRVLPDYIVMNGVESSDAGLLQELGLGGFDGLLVSIVSSSAEDCLNRLKLMIQF
                     ASPGVDAACVDALVDRLARFIVVLGFNSEGRTVVTGVYENVDAGNVKSGSPLIRIDLP
                     DGSPAGATIPAADDSTEVSSVPVEG"
BASE COUNT         2455 a         4010 c         3858 g         2473 t
ORIGIN      
        1 gctgaaccac gtgtccagca cgtcctcgtc ctggaccagt cggcggctgc cgcacttcgg
       61 gcagacggtg tccggaccgg ggtcggtttc gccgacgtac atgccgtcac aaccgggctc
      121 ctggcagtac cagaccggga tctggtgtcc ccaccagagc tggcggctga tacaccagtc
      181 gcggatgttc tccatccagt tcatgtaggt cttttcccac gaacccggga cgaactgcgt
      241 acggccgtcg cggaccgcgt cgatcgcggg gcgggccagc ggctcggcgc ggacgaacca
      301 ctgcatcgag accatcggtt cgacgatgac cccgctgcgc tggcagtggc cgacgcggtg
      361 ggcgtaatcc tcgaccttga ccagcaggcc ggcttcggcc agatcggcct cgaccagctt
      421 gcgcgcggcg aatcgctcca tgccgcggta cttttccggg acgttgtcgt tcaggttgcc
      481 cagcttgtcc aggatcgaca gaaccggcag attgtggcgc ttgccggtct cgaagtcgtt
      541 gaagtcgtgg gccggcgtga tcttgacggc gccggtgccg aattccatgt caactgcctg
      601 gtcgtctccg atgatcggga tcttcttgcc ggtcagcggc agatcgaccg tcttcccgat
      661 caggtgcgca tggcgcggat ccttcgggtg gacggcaacc gccgtgtcgc cgaacatcgt
      721 ctcgggtcgc gttgtggcca ccaccaggga ctcggacgtt ccggtcaccg ggtaagcgat
      781 gtgccacatg tgggacttca gttccttctc ctcgacttcc aggtcggaaa gcacggtctg
      841 gatgccgggc gaccagttca ccatgcgctg ggcgcggtaa atcaggcctt cctcgtacag
      901 gcgcgtgaac acgacgcgaa ccgcgcggga caggccatcg tccagggtga agcgttcacg
      961 ttcccaatcg accgacacgc ccagggcggc catctgctca acgatgcgat cgtgatggcg
     1021 gtgcttgacc tgccagacgc gctcgatgaa cttttcgcgc cccagatcga acctggacag
     1081 gccctcggtc gccttcagtt cgcgctcgac aaccatctgg gtggcgatgc cggcgtgatc
     1141 cgtgccgggc agccacagcg tgttgaagcc gctcatccgc ttccagcgga tcaggatgtc
     1201 ctggatcgtc agcgtcagcg cgtgtcccat gtgaagctgg ccggtgacgt tgggcggcgg
     1261 aatgactatg caatatggcg gcttgtcgct gacgtcggcg gcgcggaagt atccggccct
     1321 gttccagacg tcataccagc gaccttcgat ttccctgtgt tcgtaacctt tcgcgagcgg
     1381 ctccatcagg cacccctgga caaagttttc aggggcgaaa ttatacaggc aaaaggctcg
     1441 ctcggacagt atcttgacat gaacgcaggt gttcgcgggt tattgctaat atattattag
     1501 taactccaca aatctggctg gtaatgataa tatattgatt gctaactgga cttctggcgg
     1561 gacaatttca tgagctccgt taacctgaca ttgacaggcc cgtccgacat ggcagcctcg
     1621 ctggcgagcc ggatcaggga ccttcgcctg ttacgtggat ggacccaggc cacgctgtcc
     1681 cggcgcgccg gcgtgactgc atcgtcctac cgccgcttcg agacaaccgg ccatgcctcg
     1741 cttgaactgg tcctgaaggt agcgcacgcc ttgtcnnnnn nnnnngaacg agtttgaaca
     1801 actcttcgaa cccccgcctg cccggagcat cgaacaactt gaacagcgga tcacccagcc
     1861 aatccgaaaa aggggccaca tatgaaaaag ctcgaggtgc gcttcacacg cagccccggc
     1921 gattcgatac ctgtcggcac gctggcctgg gatcgtggca ggacatattt cgaatatgcc
     1981 tctgacttcc tggccaccgg cctgaacatt tcgccattcc gcctgccttt cgagcctggc
     2041 ctgttcgagc atcgcgacca cgaattcggc ccgctgcccg gcgtgttcga cgattccctt
     2101 cccgacgggt ggggcctgct gctgatggat caccatattc gctctcttgg cctgaacccg
     2161 gccggggtaa gccccctgga ccgcctggcc tggctgggca caggaaccat gggcgcgctg
     2221 acatatcacc cgccagccgg ccgggacagc ggcgacatca atatctttgt acttcatgaa
     2281 ctggcccgcc agtcgcagga gatacttgcc ggccttgctg tggatgtcct gccgcaactg
     2341 ctcagggccg gcgtgtctcc gggcggcgcc agaccgaagg tgctggtggg gtacaacccc
     2401 gccaccagcg aaatcatctc cggcgaaggt gatctgcccg agggttctga gcactggatc
     2461 gtcaaattct tcgcaaggga agaccaacct gacaccggtg ctgtcgaata cgcctattcg
     2521 ctgatggccc gcggggcagg tatcgtgatg ccgccgacgc gccttttcaa aaccgctgaa
     2581 ggcgaccgat tcttcggcat caggcgcttt gatcgcgacg gcaaccggcg ctgtcacgtt
     2641 cacaccttcg gcaatctgat ccacgcgaac ttccgaatcc catcatccga ctacaccgac
     2701 ctgctcaagg ccacgagcct gttgacacga aatcaccagg aagtgctgcg tgcatttcgt
     2761 tacatggtct tcaatgtgct gacccacaac cgcgacgacc acgtgaagaa cttcgcattt
     2821 atcctggatg acaggatcgc tggaacaggc tcgccgcagg gcggcgactg gtcgctttca
     2881 cccgcgtacg acctgctgtt cacgcatggg cctggcggcg agcactccat gacggtggcc
     2941 ggcgagggca gaagccccac tcgacagcac atgctcagag tggccgagcg ggctgatctt
     3001 tccacgcgcg aagccgacgc aatcatcgtc gaggttcagg acgcagtcgc ccggtggccc
     3061 gaccacgcat ccgccgccgg cgtaggcacg gccacaacgg aagcgatcgc ccggttcctg
     3121 acccggcttc cataaaatcg cggcagaact cccgattagc tatatgatcg ccgatgacac
     3181 gcttctgcgc cggacaggta tcagatggtc ggaacggatg acaggattgc ggcgatcggc
     3241 gggatgccgt ccgaagagga catcgtgctg ttcgggccgg gctgtcagat gggctgcaca
     3301 ttctgccccc attcgggcgg ccgtgcaggg caccgcatgg aaacagtgct ggcgcctgac
     3361 ttcaggctgc cacttgccca ccgggtgacg atactggccg gggacgtcgt caggaaagag
     3421 gtcgcgccgc tggtatccgc cctgcgctcc cagggctccg tggaagtatt cgcatacgcc
     3481 cacccgggaa cgtgggatct ggacgccctg gacgaactga cctcggccgg ccttaccgga
     3541 atccaactga tgctgcctgc ggcacgcaag gccgaacttg cggcgctgac cggcggcaag
     3601 ggttcacttg gcctggtggc gcgcctgatc gatcacgcca atcgccgggg gcttcgcgtc
     3661 gcggccgaaa tcccggtcgt ggaatcgtcc gccacattga ttgacgatac cgtccaaagg
     3721 ttcctgaaga ttaccgccct gcccgaccgc gtggcgctgc gctttgtcgc cgagttccat
     3781 ccggttcgcg gcccggttcc atgggatcat tccattgcca cgccgcacgt tgccgcggcg
     3841 tccgcgctgt gccggggccg gcaggtgccg ctgatcctgg ctcaccccga agccccgccc
     3901 ccgtgcgtga tggacgttcc cgacgccagc gccgccatgt accccggact ttccatgtac
     3961 ggccttcccc gcggggcgcc acgtcctttc gcggcctgcg ggtcgtgtgc ggccgctgcc
     4021 gtgtgtcatg ctgaaggacg ttttctgggc ggggccgagc cagtgctgac cgcgccgctc
     4081 gcgctgacag gcacggcagg aagcgatgct ccaggtccag tcgccgggat ccgtcccgaa
     4141 agatcacggt atatggccag cgaaggcact gaccgacgca aaggactgga tcagccggac
     4201 acacccgggc caaatgcttc gaccatctgg cgcaggcagg ccgatctgaa ccttctgcgt
     4261 gtcgaactcg aaaggcggcg aatcgcgtgc cgttatccat gggaacagct tgaggctcac
     4321 gatatccgtg gcgtggtgac accgtgcgcc ggcggatggc cgcttgaatc gaccgtcaac
     4381 gactgcgttt cgtggcatgg cggcagcctg ctgaccgcct ggaactcgcc cggcatgcag
     4441 aacacccgcc gcgcggtcgc cgccggccgg gcccacgaca catgcaagcg tgactgcccc
     4501 gctttccacg gcggaccgat gggcgccgtc ccttcgatgt cggccccgac gaccggcgtg
     4561 atgtttgaca acatcatcga caacgtccgc gagatgctgg acggtgcgac cgtgcttcaa
     4621 agccgcccgc agtccatcag cttctcgccg accatgcgct gcacgaaccg ctgccgcatg
     4681 tgcgacatcc acgaagtcat ggaagcaatg ggccggccgg ccgagatccg ggaaatgccg
     4741 gacagcctgt tcgacgagct tctggaactg ctgccgacga cccgcatgct ggcgctgaca
     4801 ggcggcgaac cgctggtttc gaagcgcatg cgcgaccttc tgaaaagatt ttcggccgaa
     4861 aggtatcccg acggcgctgt aaccatcacg acgaacggcc agcttctgcg ggaaaacatc
     4921 ctgcgcgacc tttctggtac caggatcagg cagttctatg tatcgctgaa cgcggccgac
     4981 gacgccatgt acgaacacgt gtccggcatt ccaggcggat tcacccgtgt aagaaaccac
     5041 cttgcccttc tgatggacat ggccccacgc atggcaggcc gccccggcgt gatcgtcagc
     5101 ttcgtcgtca tgcgttcgaa cttcacgcag cttccggagt tcctggatct ggcgctcgga
     5161 ctgaagaccg gcatccgcct gctaccgatc gagcgcgacc gcatgggcga gtcgatcttc
     5221 accgacgaac cgacgcttgc agccgtccat gccatgctga ccggcacggt gatgccatcc
     5281 atcgaacgcc tgccctgggg ctaccgcgcg gaaatcgcca ggctgttctc gatcatcgac
     5341 ggccgccttt cccgccgcga cttcagtccg ctgtaaggga tgacgttccc cgcaacacgc
     5401 cgaaccagcg gtgttttagt atcaaaaatg cgcatcaggc attaaaataa cccggtttca
     5461 attccggagg gtctgatgcc cgctcaactg gtactggcat ccgcgtcacc aagacgcctg
     5521 gaactgcttg aaaaggtggg gttttcggtc aaggccatgc ccgccatcat cgatgaatcc
     5581 cagcacctgg acgaaatgcc cgacacgttc gtcaagcgcg ttgcccgcgc caaggtcctg
     5641 accgtcgtcg atcgaatcca gcagaccctt tacacagatc cggaacaggc gcgaatccag
     5701 cgggggctgc gccgcgacgt gccgctacgg tgggtgctcg gcgccgatac cgtcgtcgta
     5761 attgacaacg acatcatcgg caagcccacc gatcacgccc atgccgtgga aatcctgacc
     5821 cgccttcagg gccgggaaca ctacgtcctt accggtttct gcctgtacga catgcagaag
     5881 tccaaggaag gcctgcaggc cgtccagaca aacgtccgca tgaaggaaat gtccgtcaag
     5941 gaaatcgaac ggtacctgac ggtcggggaa tcgatggaca aggccggcgc ctatgccatc
     6001 cagggtgtcg gcatgtacct tattgacagc ctggtcggca gctatacgaa cgtggtcggt
     6061 cttcccgtct gtcaggtcgt cgaaatgatg gacgaaatga acgcccgtga tatcctgccg
     6121 tactaggggg gatttccccg gtgcccgatt gattcccgtt cagcctggat gctgccgtga
     6181 ctcgcgaaga atcgatacgt tccggattga atgaagtaag ggacggaatc gccctggctt
     6241 gcgggcagtc cggccgctcg gtcgacgaaa tcaccctgat cgcggtatcg aagttcatgt
     6301 cggtgcagga catcgagacg gcctttaatg cagcccagct tcacttcggc gaaaactacg
     6361 cccaggaact tcttgccaag gcatcgagcc cgcagcttgc cggaaagcca atccggtggc
     6421 acttccaggg tgggctccag tcgcgcaagg ttcgcgatat cattccggtc gcatccacca
     6481 ttcagtcggt tgatcgcgat tcggtcatcg acgagcttgc caggaggacg gatcccgcgg
     6541 cccccgtcga tgtgttcctc gaggtcaata ccggtggcga ggcccagaag tcgggggctg
     6601 cccctgccaa tgccgaacgg ctgtgccggc ggctgattca aattcctggt gttcgcctta
     6661 aagggcttat gactatcccc ccatttcttg aagatcccga agcttctcgc ccgtacttcg
     6721 tggcgctgcg tcgtcttcgg gacgaactgc gcgcgcgtct tggagcatcc ggcgacgtcc
     6781 ttgccggact gtcgatggga atgacctcgg attacaaggt cgcgatacag gaaggcgcca
     6841 ccgtcgttcg cgtcgggacc gccattttcg gggcacgcac accttaacag cggggctgac
     6901 gccgggatga aggttgaacg caggttcgcg gcaggaacgg tcgaaatcct ggtgcttgtt
     6961 ttctttttcg gccttctctt cctgtattcg ttccatccga actgggacat cgacatcttc
     7021 tggcaccttg aaaccggtga gtggatttcg aataaccaca ggctgccgga caccgacatc
     7081 ttttcgtcca tcgacaagac caggccctgg atgactttcc agtgggcgta cgaggtcatc
     7141 acatatcagg tcaacgcaag gcttgggttc aaggccgtgc gactgatgca cgcgcttctt
     7201 tacatgggcg ccttcgcgct gttgtacgcg acgtaccgac gcaggctttc gtccagatcg
     7261 gtcgcgcttg ccctgcttgt gaccacactg gtcctggccg aggatcgttt cagggtacgt
     7321 cccgaggcgt tcaacttctt cttcacggcc atcgtgctgc caatactgct ggactgtgcc
     7381 aaaccaggcg cgccaaggcc caggatccgg acaatcgttt tgattgccgc cgtatcgctg
     7441 ctgtgggcga acatccacgc cgggggcgca ctgaccctgc ttgtcggctc gggggcgatg
     7501 ctcgggggca cccttctggg cctcgttctg aaccggaacg gggaatcggt catcaggctg
     7561 aagtacaacg catgggtctt tggcaccgcg gccttcctga tggccatttc accgaacttc
     7621 atcaggggca acatccacgc cttcacgatg gtcaaccaca gcaggctcct gattcccgaa
     7681 tggcatccgc ctgccgccta cttcatgccc tcgatgggcg gacagcttac cgtccacact
     7741 ttcctttgcg gaacgattcc atatcttgtc ctgggttggg cgatgctggc tttcgggctc
     7801 gccctgacgc gggcgatcgg ctccaggcgg gacaacgacc ccgatcgacg tgaaaagcct
     7861 ttcggaatcc tgctgaccgc cctcttcttc gcggtgatcg cggcccagtc gtccaggttc
     7921 gtctatacca gttcattcgc catcttcctg cttctgctgt caaagccgcg catgcttggc
     7981 cagattgcat cgaacccgag ctggcgcgtc atcttcatgc tcgttgcggg attcctgacg
     8041 ctggcatctt tccagtactc ggtggctgga aaaaaggatg gcctgaaagg gcagttccgg
     8101 atgctgagtc tcgataacga accggatcgc ttcccggaaa aggcatcgga cgcgatcgcc
     8161 gcgatgggac ttgaaggccg gatattccat ttcacggcgt ggggtggata ccttctttac
     8221 cggcatttcc ccgaatgcgc tgttttcacg gacggccgcg ggaacttcac cctggatgaa
     8281 agaaaggttc ttatcgacac gcatcggccg tggggacgcg aggacgccct tgaagaggcc
     8341 tggtcgaaat atccatttga aatccttctt cttccccgac cggttttccc gatgctgacg
     8401 tgggacagaa cgaaatggat gctgatttat cgtgacgaca tatccgaagc attccttcgg
     8461 gtaacgcctg aaaatcaaac caatatccag cgcgcgatgg cctggtggaa ggttgtcggg
     8521 atatcatcga cgagcgatcc ggttgccttc caggaagatt accgccgggt tcggggattg
     8581 attgacatca gggatcccag gctggacgca aaattgacca acgcggcgca gcaggcgcac
     8641 accgacagcc ttgcaaaccg ggtgtccggg cttcacgacg gcgcgttgat cctgttcggc
     8701 gtcgggcttc acgaaaaggc gacgaagttc ttcaggatga tccttgatga aggcatccgg
     8761 cattcgacgg ccgcgcttta ccttgcctgg tgccagtatc ttggcaaccg ggaagacctt
     8821 gcgcgtgaaa ccttgatgaa gaatttcagt tccccggata tccttggaat gccggatcgc
     8881 ggcccgttaa gacagacggg gctgaggatc ctggacgagc tcggcgccag ggtgggcctg
     8941 cggaaggcag ccccccagtg atccacccgg ggttgcgggc ggacgtccgc ttcatgggag
     9001 catcgggact gtcgggttgc attaatcttc cggcaggcat taataaggag gtcaccatgt
     9061 caaagattct ggacaccgta cttctgctgg cgcttcccgc gtcgggcaag tccgaggtgc
     9121 gcacctggct tgcgtcggtt ccaccggaaa aatgcgtcag cgacttccat atcggcgaaa
     9181 ccgtgcagct tgacgattac ccgtacgtac atttcatgcg ctgcatcgac gacgccatga
     9241 gggccgccgg ccatgccgga ccgttcttcc acgcaaacga caggcctttc aaggatccca
     9301 gggactggtg cacgcttatt gaactgctga acgaagacta cgcggccctg aagggatgca
     9361 ttcgattcac ggtaaaggat ccggtcgcat atcttttcga acgcatcgac tcggctgccc
     9421 tgcgtgccgg cgcgccggtc aggcttggcg tgcttcctgc cgacgtcgtc gatgcggtca
     9481 ggccggcgct tgaggccgag gctgcaagga tcgtcacgga tctggaagcg ggcaagcccg
     9541 actcgctgga aggacgcacc atcgttattg aattcgcacg cggcggcgcc cacggttcga
     9601 cgatgcccct gtttgaacct ttcggttacc gggcgtcatt gaagcagctt tcaccggcga
     9661 ttctgaagaa tgccggaatc ctttacatcg ccgtcacgcc ggaagaatcg cggcgcaaga
     9721 actatgagcg gacggatccg aacgacccgg gttcgatcct gaaccacggc gtgccgattg
     9781 acgtcatgat gggcgattac ggttgcgacg acatggcctg gctgctgtcc cggtcggatg
     9841 tccccggaac ggttcgcgtc gaaagggccg gccaggtatt ccacgtgcct ctttcgatat
     9901 tcgacaataa cggcgaccgg acgacgttcg taaggcgcgg tcaggcaaac tggaagaagg
     9961 aagaagtcga atacctgcgc gacgggctca aggcagcctt cgaccacctc gccggatccg
    10021 acaggttcta gcgaccaccg gcgatcccgc caatcttcca gccggcaatg gtcacggcaa
    10081 ccgcggcgac aaggattgcc acgacccata tcttccagcg gttggcttcc gcccacaaca
    10141 ccagcggatg gaacataatc ggggacggca tctgagacaa agtcgcttcg ttcggaagtt
    10201 caaccaccaa ctcgacggtg ccaagccgaa ggacgtcccc ctgggccagc ctggcaacct
    10261 tgaccggaac tccgttgacc tcgacgccat tggaactgcc aagatcgttg atgaccaccg
    10321 tcttgtcatg cctcctgaca acccttgcat gacggcggct gattgaagga tggacaacgg
    10381 cgacgtcggc gtcctcggca cgcccgacca gggccggcga cttggcgaag gaaagcacct
    10441 ggccctcgaa accggccgat gtgcccttca ggcgaaggta tgttccgtca tccagtcgcg
    10501 caccggaaac cgagagcacg aactccccga tgcggatcag gtcgtctatc ctgatttcgc
    10561 gcgtgccgac gatccggacg tcgttgacaa gaacgccgtt tgcacttcca agatcctgga
    10621 taagcagccc gccgtcgtga acgacaaagg ccgcgtgagt cctggatacc cgctcggaat
    10681 caagcacgat atcgcacgaa tcggcccgac cgatgatgaa acgtccagac ctgaatgcat
    10741 actctgaagt gcccccgcca agggagttcc tgacggtgac cttgaacagt ggaagatttt
    10801 ccgggctacc cataaaccgc cacgagcccc cggacaccgg gaacctggcc ggggtcagcc
    10861 ttcaacagga acactggaga cctcggtcga atcgtccgcc gcagggatgg tcgcgccggc
    10921 ggggcttccg tccggcaggt caatcctgat aagcggcgag ccggacttca cgttgccggc
    10981 atcgacgttt tcatacacgc cggtcacgac cgtgcggcct tcggaattga agccgagcac
    11041 cacgatgaac ctggcaagcc tgtcaacaag ggcatccacg caggccgcat ccacgccggg
    11101 actggcgaac tggatcatca gcttcaaccg gttcaggcag tcctcggccg acgacgagac
    11161 gatgctgaca agcaagccgt cgaaaccgcc aaggccaagt tcctgcagca gaccggcgtc
    11221 cgagctttca acgccgttca taacgatgta gtcgggcagc acgcggctga tgatctgggc
    11281 ggcgcccgag gacgcctggg caagtgcgtt ccgcctgatg gaaaccaggt tcggcaggtt
    11341 gatgtcgatg tcgcgacggc cgccaagggc aagaacgcgg gactcggctc cgaccatcat
    11401 cgccagggaa ttcgtgatca cgcaacgcgt ggtgtatggc gggccacaga cgatgatgtt
    11461 tccgcgtgcc tcgacagcgg ccttcaaagc ctggatggtc ttttcggaaa gccccgccga
    11521 ttccttcagt tgcagttcgg tcatcgcggc ggccatcggg cgctcgatga ctataatcct
    11581 tccctgatca atgaacggcg cgccaagtat ctgaagtgtc gaaccgtcgg gcagacggcc
    11641 ttcgaccagc ggctgcgaca acgcctggga accgatcccg gccagcgaac acagcttgac
    11701 tgccacgcga ttgaaagtgt tgaagtttga gaatacttcg ttgatcgtcc ttgaacctgc
    11761 gtcctcgatt acctggatgc cttcaagtcc gccgactatg atcgccttga ttgccgggtt
    11821 ggaaagaagt tcctcgagtg ggcccaggcc ggcaaattca aacaacacct tctgggtgat
    11881 gtcgatcggg tcaacatcgg ctggaatctg gttttcgcgg cgaagcctgt caacaaggcg
    11941 catcacgccg tccgacaact tctgccattc ggcgtcggtg aagtcggcct tttccggcgg
    12001 aatggagccg aagacttccc gaccggccag ttctgaaagc gcgcgcagtg ccgcgtggcc
    12061 atccttgccg cccgagtatc caagccttct ggcaagaccg ctgaccgcgg tggccggatc
    12121 gaccgcgact ggaatgggtt ccggctggac tgccgctgcg ggaacagggg cgggtgccgg
    12181 aacaggcgcc gcgggagcga ccggggcagc cgcgggggcc ggggcagccg cgggggccgg
    12241 cgcgggtgcg ggggccggcg caggtgcagg ggcaggacgc ggtgcgggcg ccggcttcgg
    12301 cacttcaacc actttcgggg gcggtgcaac cggctgaacc ggttcaacct ggggctgtat
    12361 cggcgcaacg ggctcctcaa tttcgacttc cacctcgaac tcggtcgcgg ccgaaggctc
    12421 ctgtggcatg tccggaagct gatccaggac ggaatcgccc ggaagcggcg gcatttccat
    12481 caaatcggat gcgacatcgg ccgccgccga cggggaagcg atgtcaaaag ccggcatcgc
    12541 gatggttgcg ccgcgggctt ccgcctgttc aggctcgggc tgaacctcga ccgactgtcc
    12601 ggcatcaggc tcgacctgcg tgacctgaag cacatagtca ccgatataga cccgatcctc
    12661 gaccacaagc tcccttgggg acgaaatgcg gcgcccgttg acgtacgacc cgttggtgct
    12721 cttcaaatcc tcgaagacga tatggccgtc cgccagcctg attacggcgt gacgcttcga
    12781 cacgttcggc ttcggaagca cgattc
//
LOCUS       DAOPPA010000023        12563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5826_length_12563_cov_3.836984, whole genome shotgun sequence.
ACCESSION   DAOPPA010000023 DAOPPA010000000
VERSION     DAOPPA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_12563_cov_3.836984"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..580)
                     /locus_tag="PKH54_01410"
     CDS             complement(5..580)
                     /locus_tag="PKH54_01410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC98572.1"
                     /translation="MADQTDFEIGLHKEIQVSNFFEAIDRLAGFKAEAMLPSGRSDPV
                     LAQRIKELELYRDAFLKQVALRRPDFRVVEQSKDGSHPTVVEIGYSSGLEFYEGLSLS
                     LNRGGLFVKTEALLPIDSVLRLTVRIENLHIEFKLTAKVIWINPRETSARPVGMGLKF
                     PKLTNEQRALLEEFMQGSAPAATLVELNESP"
     gene            complement(573..1457)
                     /locus_tag="PKH54_01415"
     CDS             complement(573..1457)
                     /locus_tag="PKH54_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOC98573.1"
                     /translation="MKEERQTRVTRVNRLFTGNLVSSVCLVEIYGANLGRKYDFMTEV
                     VTIGRDPDNIIVLESDSVSRKHARIEKANQDWYIVDLGSTNGTYVNDILIMPRARLTS
                     GQFIKIGDSIFKYLNVDNLESAYHEEIYRTVVTDGLTQVANKRALGDFLERELARARR
                     HRRTLTVLMMDLDHFKLINDTHGHLTGDVVLKEVAGIVRTRIRKDELFARYGGEEFVC
                     VLPETNLEAAKTLAEDIRAAVEGHGVNFEGQEISVTVSIGAAEFDAEKHQSTTDLLME
                     ADKNLYIAKNNGRNCIHG"
     gene            complement(1522..4008)
                     /locus_tag="PKH54_01420"
     CDS             complement(1522..4008)
                     /locus_tag="PKH54_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOC98574.1"
                     /translation="MDSESNRASRDLPCPNWLARWIAFSVSRPRLVLASAALVFALSV
                     FFASKLELKTDLVELLPTDAPSVVNLETMRTRVAVHQNLAVAIECPDLKAAQKFSDDI
                     TTLIRALPPEEVLAVDNNIKEIKDYYTRNKYLFADLEDLVEVRDKIAQRIQEETEGAL
                     VESLDDEPAPRTDLKFDRLREKYESKVKIQERYVDGYYTNPDRNLVAVFIYPPSSSLD
                     TASNQKLVDKVQGFIRSLNPAAYHPEMKVGLTGEIKTGLEEREALKSDMTFVSLLCLG
                     LIALLIVVYYRSIRITAVIGVPMLLGLAAALAVTTFAIGYLNTATAFLAAIIAGNGIN
                     FMLMLTARFFEEARCNPDSTVSQDLTTSAWSTLRGTLIAGLGASIAYGSLVFAGFRGF
                     RQFGIIGGIGMILCWLATFLVGPALIAIMHRRKPMQDRQGRPSRFAPGRFFAMLVERW
                     PRFILAFALLSTAASILVILPWSFDPFEYDFRKLRNVAGYAGGSAALSKKVDKIFDLP
                     QSPTPVVTDRASDVPGMKAAILASPSSRAIIGGVKTLQDSVPDRQGEKLEVLGEIRRM
                     IDSKMDFLSPADREKVMEYRPADDLRVIGLDDVPASLARPFQESDGSRGRILYVYGRK
                     GDSLLDGKYLLKFARFIRGVKYDEGVKAIPVGQPMVFADMIDSILSDGLKVTAASFIG
                     VLLLLIFAFRKRLPVFAVMLPVVIGTVWMLGVAALMGAKLNFLNFVVIPITLGISVDY
                     GSNIISRYLQEGRGSMGRVVSSAGGAVMLCALTTIIGYATLLTSTNLALRSFGLLADV
                     GEVACILAAEVVMTAFIVWHERSTTKIRQR"
     gene            complement(4026..4235)
                     /locus_tag="PKH54_01425"
     CDS             complement(4026..4235)
                     /locus_tag="PKH54_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOC98575.1"
                     /translation="MSDCQRKEQNAARCTCSYPGCPRHAVCCECLQYHWSKKQVPGCM
                     FPPDAEATWDRSLSHFIRVWQEKGF"
     gene            complement(4267..5400)
                     /gene="ribB"
                     /locus_tag="PKH54_01430"
     CDS             complement(4267..5400)
                     /gene="ribB"
                     /locus_tag="PKH54_01430"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOC98576.1"
                     /translation="MLSTIENAISYLKAGRMIILVDDEDRENEGDLVVAAELTTPEHI
                     AFMAKKARGLICLAMDGPLCDRLGLEMMVRHNTAPLGTAFTVSLDARDGVRTGITAAD
                     RAATILKAVQDDSTRADFVFPGSVFPLRAVPGGVLSRTGQTEGSVDLARLAGLKPAGV
                     ICEILDEDGTMMRLPSLMKFGAKYDIPVVSVADIIAFRLRHEQQVREDARAELPTEFG
                     TFTVISFSTTLDSRTHLALVMGDVTTPEPVLTRVHRANFPGDVFPFIDGQGREEVAAA
                     LRIIAAEGRGILLYLNREETGEDLLGTLQRLDAEKDGEHVLRKAVGLESEMTFRDFGI
                     GAQILRTLSVRNLCVITRNPRRYSGLAGFGLNVDAFRELVENP"
     gene            complement(5432..8038)
                     /gene="mutS"
                     /locus_tag="PKH54_01435"
     CDS             complement(5432..8038)
                     /gene="mutS"
                     /locus_tag="PKH54_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOC98577.1"
                     /translation="MAGAPTPSMQQYLKAKAEHPDCMVLFRMGDFYETFYEDARDAAR
                     ILDIALTSRNRDAEDPVPMAGVPWHSVSGYIQKLIDAGKKIAICEQMEDPKLAKGIVR
                     RDVVRVITPGVLLDTELTDPSESNHLLCLLRDDRGWAAAALDASTGDFFGTFTTSDND
                     LRLFLDRLIPREALVYLPDDALLAMLPQSIRLTTSLTPADTDLGRLDADRMALFANFD
                     PSGLLGSAAASILSYVNRTHPALLPTIRPFRDTVASETMSLPNTTVRNLELLSTTSGD
                     RSRGCLFAFLNRTRTGMGARLLRQWMLAPLSDADGIRARQDRIRTLFDAPTERQRIRD
                     ALTGIPDIERLLTRLAGGSCSARDLKSLAAGIEGMRKAAWVIRETPLDALVDLADVPP
                     ALLDLATTIDRIFVQDAPSTVKDGQMIARGVSDELDRAMDMAENGRRYIAMYEAEERT
                     RSGIPKLRVKYNRVFGYTIEIPTSSASRVPADFVRRQTLANAERFTTARLSELEHQVM
                     TAEETSKELQFQMFEQWRLATLGLRTGLQQLADGIATLDVISTLAEVAHSNGWTRPSI
                     AESRVISIIDGRHPIVEASIPDGTFVPNDFVLDGTTTSMVIITGPNMSGKSTIMRQVA
                     IIVLLAHMGSFVPARQADIGITDAIYTRVGAMDDIAAGRSTFMVEMSEAAEMLSRATE
                     RSLLILDEIGRGTSTFDGVAIAWAIAEYIQDRIRCRTMFATHYHELTDLVRSIPAARN
                     QSVAVKEWGNEVLFLRKLIDGPASRSYGIQVARLAGLPDDIIIRSREVLANLENSELD
                     AVGRPMLARSRRRAGPTESTSQLDLFGASCASQPDPLVLELADIDTDRLTPIDALARL
                     ATLAQAARERKR"
     gene            8241..9794
                     /locus_tag="PKH54_01440"
     CDS             8241..9794
                     /locus_tag="PKH54_01440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98578.1"
                     /translation="MGFKGQTAVAGFGAAAEGDAGKKAGSKPSASEAKLLAGRFRLTR
                     MIASGGMGMVFLARQTSIDRDVAIKILNPDKAGDKEARDRFRVEAAAVSRLKSPHTVT
                     VFDFGEDEQGNMFLAMEFLEGRPLSTYLKERGPIEPRLVVEIADQVLDSLSEAHSAGI
                     LHRDLKPENIFVMDRSIREVFVKVLDFGVAKIIGGPTHAGTMAGKVFGTPSYMSPEQL
                     MGKSLDARTDLYSLGVVMYELLTGKLPINGRTPIEIGVRKVKDVPPRIDQVLPEAGFP
                     PGMVDFIARTVDPRIEARPTDDVEFRDLMHAAFDRRATSQVSGIHSAPSPEPGPADPS
                     RRDTVPTRAMTNPFEDVAAQDRPNRPTPRPASSGATVRPMTVAMSSRDRRKTRRMPKL
                     LGVRLMSAGQSVSATASDVSPGGGFINAREIPVVGHRIVVAFKRPDSTAFDIQIAAEV
                     TRIATGEGAQDPIRGFGVRWLRLRAPGEIEPVVKFLTKVLGIDPPTIQPPSARGAFWE
                     YSFTEGRFI"
     gene            9947..11371
                     /locus_tag="PKH54_01445"
     CDS             9947..11371
                     /locus_tag="PKH54_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOC98579.1"
                     /translation="MLFPTAEYVLFFLPVLLVAWLSRRWLATHKVFLLLASYFFYGFW
                     DWRFLPLLIVISVIAWVSTRALQATDDVRSRKVRLGIGVTVSLLILAFFKYLGFATGI
                     VVNALAAIGIHWSPILPEIALPVGVSFFVFHGISLMMDGYRAKLDRKAPFLDSLLYIA
                     FFPQLVAGPILRAASFLPQLRVQRDPGSIDVSRGMILIVIGLVKKVLIANHLATAIVD
                     HVFETPVARSGLEVLLGIYGYAAQIYCDFSGYTDIAIGCALLLGYQFPKNFDSPYIAA
                     SPQEFWHRWHISLSSWLRDYLYIPLGGSRKGPVRIHVNLMITMLLGGLWHGAAWNFVF
                     WGALHGGALVVHRLWSDSSLPFIRKIRAGRAWPFLARVICFHFVCFGWVFFRAPTWQH
                     SWQILKSLSNAWTVGPWFTGGILIAIVAGVLAQGLPVRVREYVEAGFSRRPLVMQGFL
                     LALAVLLIDALGPSGIAPFIYFQF"
     gene            11378..12538
                     /locus_tag="PKH54_01450"
     CDS             11378..12538
                     /locus_tag="PKH54_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HOC98580.1"
                     /translation="MMVRTSIDGGIATERRFYGTRDLAIVTSSFALCLLLLEGGGLVT
                     WASRLEAGPAQDRWLAVLEPLESGLDAVGLTWPRKALISLADTVGSSMHFVAADSAAS
                     GDMVDMDRIVKRHHDQAPVGDGGGAAMAGAGGGGVSRTCGAAGTVESSPVQADPAAPE
                     PVAVAIAAPEAASKAPASDKRTVILVGDSMMAVGLAPSLVRSLEKDGRFNVVRGHKSA
                     TGLSRPEIYSWPEAYSAILAEYRPDFVVCAMGGNDAQNVRYNGKVLKFGTPEWDDLYR
                     ARVREMAKLFSKGQTRTLWVGLPVMRAESFRKKIQHVNSLVKKVLEEFPTIDYVESSP
                     AVSMPDGSYASQLQDAKGRMVKMRAADGIHLSDEGGKAIAKIVIKWLTGRMP"
BASE COUNT         2436 a         3699 c         3768 g         2660 t
ORIGIN      
        1 ccttttacgg ggattcgttc agttcaacca gggtcgcggc gggtgccgaa ccctgcatga
       61 attcttccag aagggcacgt tgttcgttcg taagcttggg gaatttcagt cccattccaa
      121 caggcctggc ggaagtttcc cgtggattga tccagatgac cttggcggtc agcttgaatt
      181 cgatgtgcag gttttcgatc cttaccgtca ggcgaagcac gctgtcgatc ggcagcagcg
      241 cttcggtctt cacgaaaagc ccgccccggt taaggcttaa agacagccct tcataaaatt
      301 ccaggcccga actgtatccg atctcgacca cggtcggatg gctgccgtcc ttgctctgct
      361 cgaccacccg gaagtccggc cgccgcagcg caacctgttt caggaaggcg tcgcggtaca
      421 gttccagttc cttgatgcgc tgggccagca ccggatccga gcgccccgaa ggcagcatcg
      481 cctcggcctt gaagccggca agcctgtcga ttgcctcgaa gaaattcgat acctggattt
      541 ccttgtgcag cccgatttcg aagtctgtct gatcagccat gtatgcagtt cctgccgttg
      601 ttctttgcaa tatacaggtt cttgtcggcc tccatcagga ggtctgtggt cgactggtgc
      661 ttttccgcgt cgaattcggc cgcaccgata cttaccgtca ccgaaatttc ctggccctcg
      721 aagttgacgc cgtgcccctc gaccgcggca cggatgtcct cggccagggt ctttgccgct
      781 tcaaggttgg tttcgggaag aacacaaacg aattcctcgc cgccgtacct ggcaaaaagc
      841 tcgtccttgc ggatgcgggt ccggacgatc cccgccactt ccttcaggac cacatcgccg
      901 gtcaggtggc cgtgcgtgtc gttgatcagc ttgaagtggt caagatccat catcagcacc
      961 gtcagggttc gcctgtgccg ccttgcacgg gccagctcgc gttccaggaa gtcgccaagg
     1021 gcgcgcttgt tcgcaacctg tgtaaggccg tccgtgacga cggtacggta aatttcctcg
     1081 tgataggccg actcaaggtt gtcgacgttc aggtacttga agatgctgtc gccaatcttg
     1141 atgaactgcc cggacgtcag tcgcgccctt ggcataatca ggatgtcgtt gacgtaagtg
     1201 ccgttcgtgc ttccaaggtc aacgatgtac cagtcctggt ttgccttctc aatcctggca
     1261 tgcttccggc ttaccgaatc ggattcaagc acgattatgt tgtcgggatc ccgaccgatt
     1321 gtcaccactt cagtcatgaa gtcgtacttg cgacccagat ttgccccgta aatttcgaca
     1381 agacagaccg aactgacaag attgccggta aacagcctgt tgacccgggt taccctggtc
     1441 tgtcgttctt ccttcacggt cccccccgcg aaatccgttt gcaacgaata taccccaaac
     1501 agtcgatttg aaaagcggat atcagcgctg ccggattttt gttgtcgaac gttcatgcca
     1561 gacgatgaat gccgtcatga cgacttcggc ggcaaggatg caggccactt caccgacgtc
     1621 ggcaagcagg ccgaagctgc gcagtgcaag gttggtcgaa gtcagaaggg tcgcgtaccc
     1681 gatgatcgtg gtcaacgcac aaagcatcac ggccccgccg gccgaagaaa cgactcgccc
     1741 catgctcccg cgcccctcct gaagatagcg gctgatgatg ttcgaaccgt aatcaacgct
     1801 tatcccaaga gtgatcggta tcacgacgaa gttcaggaag ttcagcttgg cccccatcag
     1861 cgccgcgacg ccaagcatcc agactgtccc gattacgacc ggaagcatta ccgcgaatac
     1921 cggcagccgc ttcctgaagg cgaatatcag gagcagcagc acgccgatga acgatgcggc
     1981 tgtcactttc agcccatccg acaggatcga atcaatcatg tcggcgaaga ccatcggctg
     2041 gccgacagga atggccttaa cgccttcgtc gtacttcacc ccgcggatga agcgggcgaa
     2101 cttcagcagg tactttccgt caagcaggct gtcgcctttg cgaccataga cgtaaaggat
     2161 gcggccccgg cttccgtcgg actcctggaa aggcctggca agggacgccg gcacatcatc
     2221 caggccaatg accctcagat cgtctgccgg cctgtactcc atgacctttt cacgatccgc
     2281 cggggacagg aagtccatct tcgaatcgat catccgcctg atttcgccca gcacttccag
     2341 cttttcgccc tgacgatccg gcaccgaatc ctgcagtgtc ttgaccccgc cgatgattgc
     2401 gcgcgaagac gggctggcaa gaatcgccgc cttcattccc ggaacgtccg aggcgcgatc
     2461 cgtcacgacc ggggtggggc tctgcggcag gtcgaaaatc ttgtcaacct tcttcgaaag
     2521 cgcggcggag ccacctgcgt aaccggcgac gtttcgaagc ttcctgaagt cgtattcaaa
     2581 cgggtcgaaa gaccacggaa gaatcaccag gattgatgcc gccgtcgaaa gcagagcgaa
     2641 agccagaatg aatcttggcc atctttcgac cagcatcgcg aagaagcgtc ccggcgcgaa
     2701 ccgggatggt ctgccctggc gatcctgcat cggtttccgt cggtgcatga tggctatcag
     2761 ggccggtccg accaggaagg tggccagcca gcacaggatc atgccgatgc cgccgatgat
     2821 tccgaactgc cggaagccgc ggaagcccgc aaacacaagc gagccgtatg cgatggatgc
     2881 ccccagaccc gcaatcaggg ttccgcgaag cgtgctccac gcggacgtcg tgagatcctg
     2941 ggataccgtc gaatccgggt tgcatcgggc ttcctcgaag aacctggccg tgagcatcag
     3001 catgaagttg atgccgttgc cggcgatgat cgcggccagg aaggcggtgg cagtattcag
     3061 gtacccgatc gcgaaggtcg tgaccgcaag tgccgcagca aggccaagca gcatcggcac
     3121 cccgatgacc gccgtgattc gtatgctgcg gtaataaacg acgatcagca gggctatcag
     3181 tcccagacac agaagcgaca cgaacgtcat gtcggacttc agcgcctcgc gttcctcaag
     3241 accggtcttg atttcaccgg tcaggccaac cttcatttcc ggatgataag cggcgggatt
     3301 caggcttctg atgaagcctt gaaccttatc gacaagcttc tggttgctgg ccgtgtccag
     3361 cgaacttgac ggagggtaaa tgaagaccgc gaccaggttg cgatccgggt tcgtgtagta
     3421 tccgtcaacg tacctttcct gaatctttac cttggattcg tatttctcgc gaagcctgtc
     3481 gaatttcaga tctgttctgg gcgccggttc gtcgtccagc gattcgacca gggcgccttc
     3541 agtctcctcc tggatgcgct gggcgatctt gtcgcggacc tcgacaaggt cttccagatc
     3601 cgcgaacagg tacttgttcc gggtgtagta gtccttgatt tccttgatgt tgttatcaac
     3661 cgcaaggact tcctcgggcg gaagtgcccg gatcaatgta gtgatatcgt cggaaaattt
     3721 ctgggccgcc ttcaggtcgg ggcattcgat ggccaccgca aggttctggt gaaccgcaac
     3781 tcgagtgcgc atcgtttcca ggttcacgac cgaaggggca tcggttggaa gaagctcgac
     3841 cagatccgtc ttcagttcca gcttcgacgc gaaaaacacc gacagcgcga aaacaagcgc
     3901 ggcagaggcg agcacaagtc ttggccggga aacggaaaat gcgatccacc tggcaagcca
     3961 gttcgggcag ggcaaatccc ttgaggcccg gttcgattca ctgtccatcg ttcatccttc
     4021 gcgggtcaga aacccttttc ctgccagacc cttatgaaat gggaaaggct gcgatcccag
     4081 gtggcctcgg catcgggcgg aaacatgcag ccgggcacct gttttttcga ccagtggtat
     4141 tgcaggcact cgcagcagac cgcgtggcgc gggcagccgg ggtatgaaca tgtgcacctg
     4201 gcggcattct gttcctttcg ctgacagtcg gacatgctca ccacctcata gttcagggaa
     4261 cttacgtcat ggattttcaa ccagctcccg gaacgcgtca acattcaggc cgaatcccgc
     4321 aagtcccgaa tagcgccgtg gatttcgtgt aatgacgcac aggttgcgga ctgaaagggt
     4381 acgcagaatc tgcgccccga tgccgaagtc acggaaggtc atctcggatt ccaggccgac
     4441 cgccttgcga agaacgtgtt ccccgtcctt ttcagcgtca agccgctgca aggtgccaag
     4501 aagatcctcg cccgtctcct cgcggttcag gtaaagcagg atgcccctgc cttcggcggc
     4561 aataatccgc agtgccgcgg ccacttcctc gcgaccctgc ccgtcaatga acggaaagac
     4621 gtcgccggga aagtttgccc ggtggacacg cgtaaggaca ggttccgggg ttgtcacgtc
     4681 gcccatcaca agcgccaggt gcgttcggct gtcaagcgtg gttgaaaatg aaatgaccgt
     4741 gaatgtgccg aattcggtcg gcagttccgc ccgtgcatct tcgcggacct gctgctcgtg
     4801 gcgcagtcgg aacgcgatga tgtcggccac cgataccacc ggaatgtcgt acttcgcgcc
     4861 gaacttcatg agcgacggaa gacgcatcat ggtgccgtct tcgtcaagga tttcgcagat
     4921 tacccccgcc ggcttcagcc cggcaagcct tgcaagatca accgagcctt ccgtctggcc
     4981 cgttcttgac agaaccccgc cgggcacggc gcgcagcggg aagacggaac ctggaaaaac
     5041 aaagtcggcc ctggtcgaat cgtcctgaac cgccttcagg attgtcgcgg cccggtcggc
     5101 cgcggttatt ccggttctaa cgccgtcacg ggcgtcaagc gaaaccgtaa aagcggttcc
     5161 gagcggcgcg gtgttgtggc gcaccatcat ttccaggcca agcctgtcgc aaagcggacc
     5221 gtccatcgcg agacagatca ggccccgggc ctttttagcc atgaaggcga tgtgctccgg
     5281 cgtggtcagt tcagcggcga cgacaaggtc gccctcgttt tcgcgatcct cgtcatcgac
     5341 cagaattatc atccggccgg ccttcagata actgatggcg ttttcaatcg ttgagagcat
     5401 gtcggtttcc tgcaatattc aaagcttccg gtcagcgttt tcgttccctt gcggcctggg
     5461 ccagcgtggc cagcctggcc agcgcgtcga tcggagtcaa cctgtcggtg tcgatatcgg
     5521 ccagttccag caccagcggg tctggctggg atgcacagct tgccccgaaa aggtcgagct
     5581 gtgaagtgct ttccgtcggg cccgcgcgcc ttcttgatct ggccagcata gggcgcccga
     5641 ccgcgtcgag ttccgaattt tcaaggtttg caaggacttc cctggatctg atgataatat
     5701 cgtccggaag tccagccagc cttgcaacct gaattccata ggaacggctg gccgggccgt
     5761 cgatcagctt gcgaaggaac agcacttcat ttccccactc cttgacggcc acgctctggt
     5821 tcctggccgc cggaatgctt cgcaccaggt cggtaagctc gtggtaatgg gtcgcgaaca
     5881 tggttcgaca acgaatcctg tcctggatgt actccgcgat ggcccatgcg atggcgacac
     5941 cgtcgaaggt cgatgtgccg cgcccgatct cgtccagaat cagaaggctg cgttccgtgg
     6001 cacgcgacag catctcggcc gcctcggaca tttcaaccat gaacgtgctg cgtccggccg
     6061 caatgtcgtc catggcgccg acccgggtat aaatggcgtc ggtgatgccg atgtcggcct
     6121 gccttgcggg cacgaagctg cccatgtggg ccagcaggac gatgatcgcc acctgccgca
     6181 tgatggtcga ctttccggac atgttgggcc cggtgatgat caccatgctg gtcgtggtgc
     6241 cgtcgagaac gaagtcgttc ggaacgaacg ttccgtcggg gatggatgcc tcgacgatcg
     6301 ggtgacgtcc gtcgatgatt gagatgaccc tggattcggc gatcgatggc ctggtccagc
     6361 cgttggaatg ggccacttcg gcaagcgtcg aaatcacgtc aagcgtcgcg atgccgtccg
     6421 caagctgctg gagtccggta cgcagtccaa gcgtggccag gcgccactgc tcgaacatct
     6481 ggaactgcaa ctcctttgaa gtttcctcgg cggtcatcac ctggtgctca agctcggaaa
     6541 gtctggccgt ggtgaaccgt tccgcgttcg ccagggtctg gcgcctcacg aagtcggctg
     6601 gaacccgtga cgcgctggag gtcgggattt caatcgtgta accgaaaacc cggttgtatt
     6661 tcacgcgcag tttggggatg cccgagcgcg tcctttcttc cgcttcgtac atcgcgatgt
     6721 aacggcgccc gttttccgcc atgtccatgg cgcgatccaa ctcgtccgaa accccgcggg
     6781 cgatcatctg gccatccttg acggtcgatg gcgcgtcctg gacgaaaatc ctgtcaatgg
     6841 tcgtcgccag atccagcagg gcaggcggaa catcggcaag atccacaagc gcgtcaagcg
     6901 gggtttcccg tattacccag gccgccttgc gcatgccttc gattccggcc gccagcgact
     6961 tcaggtcgcg ggccgagcag cttccgccgg caagccttgt cagcaggcgt tcgatgtccg
     7021 ggatgcccgt aagcgcatcc cttatgcgct gtcgttcggt cggggcgtca aacagcgtcc
     7081 tgatccggtc ctggcgggca cgtatcccgt cggcgtcgga aagcggggca agcatccact
     7141 ggcgcagaag ccgggccccc attcccgttc tggttcggtt cagaaaggcg aacaggcaac
     7201 cgcgcgatcg atcacccgaa gtggtggaaa gcagttcaag gttgcgaacc gtcgtgttgg
     7261 gaagggacat cgtttccgat gcgacggtgt ccctgaatgg ccggatagtc ggcaaaaggg
     7321 ccgggtgcgt ccggttgacg tacgaaagaa tcgaggcggc ggccgatcca agcagcccgg
     7381 acggatcgaa gttggcgaac agcgccatcc ggtcggcgtc cagccggccc aaatcggtgt
     7441 cggccggcgt cagcgatgtc gtcagtctga tggattgcgg aagcatcgca agcagtgcgt
     7501 cgtccggaag atagaccagg gcttcccgcg gaatcaggcg gtccaggaac agccggagat
     7561 cgttgtcgga cgtagtgaaa gttccgaaga aatccccggt tgacgcgtcc agggccgccg
     7621 cggcccagcc acggtcgtcc cttaagaggc acagaagatg gtttgattcc gacggatccg
     7681 tgagttcggt gtccagaagg acgcccgggg ttatcacccg gacaacgtcg cgccgcacta
     7741 ttccttttgc cagctttgga tcttccatct gctcgcagat ggcaatcttt ttgccggcat
     7801 cgatcagctt ctggatgtaa ccggaaaccg agtgccatgg caccccggcc atcggaaccg
     7861 gatcctcggc gtccctgttc cgtgaagtca gcgcgatgtc caggatcctg gcggcatcgc
     7921 gggcgtcctc gtaaaaggtt tcatagaagt cgcccatcct gaaaagcacc atgcagtcgg
     7981 ggtgttccgc cttggccttc aggtactgct gcatcgatgg tgtgggggcg ccagccatgg
     8041 cgcgaaggat atcgcaaaag tcatcatttg ataacgctcg gttcgccgct tattagacag
     8101 gttttctggc tgacgatatt gacgtcggtt cgttaaatca ccatatttgc gcaaacatca
     8161 gttcatggtt tcgacaatgc gaataatcgc atgaaggcga atcgactgat ggcgactcgg
     8221 ttcaattgtg gtggttaaca atgggattca agggacagac ggcggttgcc ggatttggcg
     8281 cggccgccga aggggatgcg ggaaagaagg ccggctcgaa gccctcggcg tccgaggcca
     8341 agctgcttgc ggggcggttc aggctgacgc gcatgatcgc gagcggtgga atgggaatgg
     8401 tgttcctggc caggcagacg tcaatcgatc gtgacgtggc cataaagatt ctgaatccgg
     8461 acaaggccgg cgacaaggaa gcgcgggatc gtttccgggt cgaggcggcc gccgtcagca
     8521 ggctgaaaag tccgcacacc gtgaccgtct ttgatttcgg cgaagacgaa cagggaaaca
     8581 tgttcctggc catggaattc ctggagggcc ggccactttc gacctacctg aaggaaaggg
     8641 gcccgatcga accccgtctg gttgtcgaaa tcgccgatca ggtgcttgat tcgctttccg
     8701 aagcccattc ggctggaatc ctgcaccggg atctgaagcc ggaaaatatc ttcgtaatgg
     8761 atcgttcgat tcgcgaagtc ttcgtgaagg tgctggattt cggcgtggcc aagattatcg
     8821 gcgggcccac gcacgccgga acgatggccg gcaaggtctt cgggacgcct tcatacatga
     8881 gtcccgagca gttgatggga aagtcgcttg atgcaaggac cgacctgtat tcgcttgggg
     8941 tcgtgatgta cgaactgctt accggcaagt tgcccataaa cggaagaacc ccgatcgaga
     9001 tcggcgtcag gaaggtcaag gatgttccgc cgcgcatcga ccaggttctg ccggaagccg
     9061 gatttccgcc gggaatggtc gatttcatcg cgcggacagt cgatccgcgg atcgaggcca
     9121 gaccgaccga tgacgtggaa tttcgtgatc ttatgcatgc ggcgttcgat cgtcgggcga
     9181 cctcgcaagt gtcgggaatc cactccgcgc cgtcgccgga accaggcccg gcagacccgt
     9241 cccgccgcga tacggtgccc acaagggcaa tgacaaatcc gtttgaagat gttgcggccc
     9301 aggacagacc gaatcgtccg acccccaggc cggcctcgtc cggggcgacg gtcaggccga
     9361 tgacggtcgc gatgtcctcc agggatcggc gcaagaccag gcgcatgccc aagttactcg
     9421 gggtaaggct gatgtccgcc ggacagtcgg tttcggcgac cgcgtccgat gtcagccccg
     9481 gcgggggttt catcaacgcc cgcgagatac ccgttgtcgg tcatcgaatc gtggttgcct
     9541 tcaagaggcc ggattcgacg gccttcgata tccagattgc ggccgaggtc acccgtatcg
     9601 ccactgggga gggcgcgcag gatccgatcc ggggcttcgg ggtgaggtgg ctacgcttga
     9661 gggcgcccgg ggaaatcgag ccagtcgtga agttccttac aaaggtgctg ggaatcgacc
     9721 cccccacgat ccagccgcct tcggcccgtg gtgccttctg ggagtacagc ttcaccgagg
     9781 gccggttcat ctgatacgcc gcaatccgct tccgtcgccg tcgaacatgc tggtgatgcc
     9841 tgtatgggtt taaccgctgg aaatggtctg ctggttaatt gcgccgtggt tttggcagcc
     9901 gtcctgaacc cgtgatagag atctcccttc cagggagatt cgggacatgc ttttcccaac
     9961 cgccgagtac gtgcttttct ttcttccggt cctgctggtt gcctggctct ccaggcggtg
    10021 gctggcaacc cacaaggtat ttctgcttct tgccagttac ttcttctacg gattctggga
    10081 ctggcgattc cttccgcttc tgattgtcat ttccgtaatc gcctgggtat cgaccagggc
    10141 gctgcaggcc accgacgacg ttcgaagcag gaaggtcagg cttggaatcg gcgtgactgt
    10201 gtcgctgctt atcctggcgt ttttcaagta cctcgggttc gcgaccggga tcgtcgtcaa
    10261 cgccctggcg gccatcggta tccactggag cccgattctt cccgaaatcg ccctgccggt
    10321 cggcgtctcc ttcttcgtct ttcatgggat ttccctgatg atggacgggt atcgcgcgaa
    10381 actggacagg aaggcgccat tccttgattc cctgctttac atcgccttct tcccgcagct
    10441 tgtggccggc ccgatcctgc gggcagccag cttcctgccg caacttcgtg ttcaacgcga
    10501 tcccggctcg atcgacgttt cccgcgggat gatcctgatc gtcatcggac tggtcaaaaa
    10561 ggtgctgatt gccaaccacc ttgcaaccgc aatcgtcgat cacgttttcg agacgccggt
    10621 cgcgagatcc gggctcgagg tgctgcttgg aatctacgga tacgcggccc agatttactg
    10681 cgacttttcg ggatacaccg acatcgccat cggatgcgcg cttcttcttg ggtaccagtt
    10741 cccgaagaac ttcgattcac cctacattgc cgcaagtccc caggaattct ggcatcgctg
    10801 gcatatctcg ctttcatcgt ggttgcgcga ttacctgtac attccgcttg gcgggagtcg
    10861 caagggcccg gtgcgcatcc atgtaaacct tatgatcacg atgctgcttg gcggcctgtg
    10921 gcacggagcc gcctggaatt tcgtgttctg gggcgccctt catggcgggg cgctggttgt
    10981 tcaccgcctg tggtccgatt ccagcttgcc gttcatccgc aagatcaggg cgggcagggc
    11041 gtggcccttc ctggcccgcg tcatctgctt ccacttcgtc tgctttggct gggtgttctt
    11101 cagggccccg acctggcagc attcgtggca gatacttaaa tccctttcaa acgcgtggac
    11161 ggtcggtccg tggttcaccg gcggcattct tatcgcgatt gtcgccggcg tgctggccca
    11221 gggcctgccg gtccgggtgc gtgaatatgt cgaggcagga ttttccaggc gcccgcttgt
    11281 catgcagggc ttcctgctgg cgcttgccgt cctgctgatt gatgcccttg gtccgtcggg
    11341 gattgccccg ttcatttact tccagttctg aggtgtgatg atggtccgga cttcgattga
    11401 tggcggaatc gcgacggaac gcaggtttta cgggacccgg gatctggcga tagttacttc
    11461 aagcttcgct ctttgtctgc tgctgcttga agggggtggc ctggtgacat gggcctcgcg
    11521 tctggaggcc ggcccggccc aggatcgctg gctggcggtt ctggagccgc tggaaagcgg
    11581 gcttgatgcg gtcggactga cgtggccaag aaaggcgttg atctcgctgg ccgacacggt
    11641 gggttcatcg atgcatttcg tcgccgcgga ttccgccgct tccggtgaca tggttgacat
    11701 ggaccggatc gtaaagcgtc atcacgacca ggcgcccgtc ggcgacgggg gcggggcagc
    11761 aatggccggg gcaggtggcg gcggtgtatc gcggacgtgc ggtgccgccg ggacggtcga
    11821 atccagtccc gtccaggccg acccggccgc cccggaacct gttgccgttg ccatcgcggc
    11881 ccctgaagcc gcttcaaagg cccccgcatc cgacaagcgg acagtcatcc tggtcgggga
    11941 ttccatgatg gctgtcggtc ttgcaccatc gctggtaagg tcgcttgaaa aggacggtcg
    12001 gttcaatgtc gttcgcggac acaaatccgc aaccggcctg tcccgtcccg aaatctacag
    12061 ttggcccgaa gcctacagcg cgattcttgc ggaataccgt cccgatttcg tcgtatgcgc
    12121 catgggcgga aacgacgcgc agaacgtgcg ttacaacgga aaagtcctta agttcggaac
    12181 gccggaatgg gacgacctgt accgcgccag ggtccgtgaa atggccaagt tgttctcgaa
    12241 ggggcagaca cgcaccttgt gggtcggatt gccggtaatg agggccgaat ccttcaggaa
    12301 gaagattcag cacgtcaatt cactggttaa aaaagtgctg gaagaatttc caaccattga
    12361 ctacgtggag tccagccccg ccgtcagcat gccggacggc agctatgcaa gccagttgca
    12421 ggacgcaaag ggccgcatgg tcaagatgag ggcagccgac ggaatacacc tttcggatga
    12481 gggtggaaag gcgattgcaa agattgtcat caagtggctg acgggaagga tgccctagtt
    12541 cgattcgatt tcaggaaggc acg
//
LOCUS       DAOPPA010000024        12435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5907_length_12435_cov_3.863893, whole genome shotgun sequence.
ACCESSION   DAOPPA010000024 DAOPPA010000000
VERSION     DAOPPA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5907_length_12435_cov_3.863893"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKH54_01455"
     CDS             <1..628
                     /locus_tag="PKH54_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOC98581.1"
                     /translation="TIQQQYLPSRWPDRRVLGMVRPSAEVLADLPPGAIPGITRPGNA
                     TGTVAILATPGTVLSRSFVIELEKLAPGLKVIQQACPMWVPLVENGELEGPGCEYFVR
                     KYLEPVLRGPQIPGRILLACTHYPLLLPRIREVVPPDIEILTQGGIVSARLMDWLDRH
                     PEHAGRLTGNGTREYLTTDDTTRFDELASMFLSTPIMSRRVHLPRLDT"
     gene            686..1816
                     /locus_tag="PKH54_01460"
     CDS             686..1816
                     /locus_tag="PKH54_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98582.1"
                     /translation="MELFLNHACNLRCTYCYNHSHSVNPMTTEVMDRALQMAFAQPVD
                     IVNIGFFGGEPLLSPDLLSKGVAQARKLASAGRRRMRLIMTTNGTLLDGPRLDFILKN
                     RFRLAVSLDGPEHVHDAHRVFASGRGSHSLVVANLKHALTLKPDISVISVSGPDNIRH
                     AGAALRFSASLGVLRHHPAIDYGACWDENALRRFARGIQGLADAWADLYRAGNPVSVP
                     LIDSKIARNIIHPQVSLPRCGCGGPEWTVGPSGRIYPCDRMVGPDDGSGPFIGDVFSG
                     IDPLLQREFLDCHHSTPDCCAGCADAHRCSFWGSCIKFNMTGRITSTPPAELCRMEKA
                     IMRAADRVAGELWTEGNREFIRRFYEDERARLAMSRCNPSGA"
     gene            1905..2996
                     /locus_tag="PKH54_01465"
     CDS             1905..2996
                     /locus_tag="PKH54_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOC98583.1"
                     /translation="MRNSVAFVIALLAASHAVAQPAPQPTKQPAAPAPSPDGQGVYGL
                     YCSGFAAGSEKMFSDIVEIIDRTYLNAVVIDVKGDSGRLSYKSEVPLARKYGSGQAKI
                     KDITGLIRTLKDRNIIAIARIVAFKDQWMAEKNQAWAVRDIDGGVWKDRKGAAWIDPY
                     NRKYWDYLVEIAEEAVRHGFVEIQFDYVRFTSDGDTSRCVYPARTRTSKQDLIAQFLE
                     YASRRLRPSGVKVSADIFGLATSTPGDMGIGQNLEKIALNTDIVCPMVYPSHYGAGNL
                     GIADPDRNPYRTVFRGVSDGMARLDAAGLKTMMRPWLQAFSIKNRYGKAQIADQVRAT
                     IDAGAREWLFWNAGCVYKLEWFQEPSLRR"
     gene            complement(2982..3494)
                     /locus_tag="PKH54_01470"
     CDS             complement(2982..3494)
                     /locus_tag="PKH54_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HOC98584.1"
                     /translation="MDAFMNRVLDLLPARSRIGLWMWWFGHFKIPLVGHVGPRIVEAG
                     DERVVVVVPLRRRSRNHLGSLYFGALALAADVTAALPALARMQERGYRISLVFKDFSA
                     QFLKRADGDTHFTTDQVAEIREFVDRVAVSGQRENLPVRVVATVPSRYGQEPVASFVL
                     TLSMKASSSK"
     gene            complement(3637..4818)
                     /locus_tag="PKH54_01475"
     CDS             complement(3637..4818)
                     /locus_tag="PKH54_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOC98585.1"
                     /translation="MGRFLVRYSLPATDRGDAEAQAFALAVEQTIEFPYDLVTDTYVK
                     GEITGRVESVELYQAVTGDLSDIPGYSLPVGMRRYLIAVSYDEATAGTEFSQFMNVVF
                     GNSSMKPGVRVESINPTAVQAAIFRGPGFGIEGIRQAVGVFSRPLLCSAIKPMGLDVD
                     TLADMAYRFALGGIDFIKDDHGLADQRFSPFVERVSRCMEAVDRANRETGRRCLYIPN
                     VTADGPATLERAIFARRAGAGAVIVSGGLTGLSMLRSVATSAEVGLPVFFHPAFSGSY
                     VVGPSPAFTERAFFGQVLRLACADAVIFPSFGGRFSFSRKQCVGILSGCRMPMSGLKP
                     MFPAPGGGLNFSNISDSMGFYGNDVIFLIGGGLFRHSRDITLSVRDLMALVESGASPK
                     P"
     gene            complement(4821..>5758)
                     /locus_tag="PKH54_01480"
     CDS             complement(4821..>5758)
                     /locus_tag="PKH54_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOC98586.1"
                     /translation="LSADLTGSCEADGFRDAYPRRFISCGIAEQNMLSVAGGLCREGF
                     IPLVHTFAVFICRRAFDQVAMSVAYPNLPVKMFGFLPGITTPGGATHQAIDDVALMRS
                     LPNMTVLETGDATEVESVLDLAYSIDGPVYVRMLRGDVPRLFPTDEPIRADHPRILSL
                     GDEVTVFSSGICTEEVLRASAVLKARGVSITHVHISTLKPFASGLIDQAIRAGSRGIV
                     TVENHLVDGGLGTAVASRCASTGVGRPLVRLGLQDTWSHGAGRAALMREHGFDAMALV
                     NAVQDLIGANLSISLADLGAVRVEPVHSVAKAEAL"
     assembly_gap    5759..5768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5841..6758)
                     /locus_tag="PKH54_01485"
     CDS             complement(5841..6758)
                     /locus_tag="PKH54_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOC98587.1"
                     /translation="MAGGWKVDVALLADRVRRAVLEHTLDNDGGYMSQACSSAELLAT
                     LYGRFMNIGMPDRPIEPRPWLGTPRHGNTEYRTGADFNGGVHDARDRFILSPTHYSLV
                     LYALLVETGRMSPQGMKQFNRDGSSVEMIGAEHSPGMEVMTGSLGQGLAQAAGMALGR
                     KLKGASGRVVVMMSDGEFQIGMTWESIQFMAHHHLDNMVVFVDVNGQQCDGAVDSVMR
                     IDPIDVRIRSFGARAAVVDGHDPAAIMKAGSRAPDGRPLFVLAMTDPCRGLDLMRKNA
                     PKLHYLRFRTPQEKAGYKAVLDAWPKRGS"
     gene            6918..8606
                     /locus_tag="PKH54_01490"
     CDS             6918..8606
                     /locus_tag="PKH54_01490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOC98588.1"
                     /translation="MRTTALRKPGRHAHFNQMTAAALAAIALVSFGQPQRADACTNIL
                     VTKGASADGSTMISYSADSHDLYGFLDIKPAGTHEPGTMIDIIDWDTGKKLGQIPQAP
                     VTWTVVGNINQHQVAIGETTFGGRPELEGPAGLIDYGSLMYLALQRSTTAREAIKVMT
                     DLVAEYGYHSTGESFSISDPDEVWIMEMIGKGKDRKGAVWVARKIPDGYVSAHANQAR
                     IRQFPQDDPENTIFSPDVISFAREMGWFCGDDRDFSFADAYAPPDYGALRFCEARVWN
                     VFNHIRPSEKIPADFVKAVPGASPMPLWIKPDRPVSVKDVMTEMRDHFEGTEFDLSRG
                     VGAGPFGLPYRWRPLQFKVGEKEYFNERSIATQQTGFSFVAQCRGWLPDPVGGILWFG
                     VDDTASSVYVPMYAGITAAPRGFGRESGNFNEFSWDSAFWVFSAVAEKAYSRYSDIIK
                     DIRTVQTELESSFHSMVPQVDETAVRLHGASPELARAWLTAFSATVGDVTMKRWRDLW
                     GRIMVKYNDGNVRNDKGEIEHPPLPDEWYRRIIQERPGFFEVLPVEAAKGPSCR"
     gene            8622..10007
                     /locus_tag="PKH54_01495"
     CDS             8622..10007
                     /locus_tag="PKH54_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC98589.1"
                     /translation="MQETTSGNRFPRSFWTANVTELFERGAYYAMASFVVIYLGQLGL
                     GDYWPSTLNGILWTIIYFLPILSGTIADQVGFRKSLLLAFVLVAAGYACMSYPVLIAG
                     HELSDIVKPEVTASMDTIVAIIAGIVLIGIGGSFVKPCISGTVQKTSVGRATLGFAIF
                     YMVINIGSLFGRGASYIVRTKFNNISLIFVVAAACAILALLVVAFLYRDPESETGTGN
                     AAKPRRSIGEILLNMILVLKNVRFALFLLVSSGFYFLYAQVYNVLPLYLKKVVETNPA
                     VDIYTMANPIVIVLFQLLITRLFGKMKPIRSIVIGTVIIGLSMAINLVPVFTSGVRVQ
                     VLDLLPFGSAMIVLTVAMIAFGELFTSARTYEYIGALAPKGQEGLFLGYANLPMAIGA
                     LIGGPAGAAIFNEIMARDAKVLDNGLLELNPVNNAIGWVVLMGIGILSALSMWLYNRW
                     LIRANAAAENA"
     gene            10107..11531
                     /locus_tag="PKH54_01500"
     CDS             10107..11531
                     /locus_tag="PKH54_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HOC98590.1"
                     /translation="MNQMRFMFLFAAIFGLVVGCGGGDKVPTGDLGPADTIQSDTAPD
                     TIVPEDVPVGDVQNHDTNVPDVPVGDVPVGDVPVGDVPVGDVPVGDVPVGDVPTDENT
                     PVDNGGDTVTDSVGPVACTTNAECTEEQFCKVNTCGEAGLCEPTPALCVVPLISENLC
                     ACDGLYYESTCFANMARTNVANGASCGSETTCSDNSGCQELEYCLKSTCGAAEGSCSL
                     KPQMCPITPNPVCGCDGVTYNNACTAAASGANIDSTGAACATVTTCTSDADCTVGFCF
                     KYSCEAAAEGTCETKPPFCLVIGDGVCGCDGNSYTNECLAKKAGVSVDPTGDACDATE
                     TCASNADCTSTQYCKKTTCGAAEGVCTAVSIKPCSDLGDAVCGCDGVVYPNACKAAKC
                     RTNTQKIAACTSTDTCSVKDTCLRGDYCLSANCGSGVCMPVPKACETATYQVCGCDDI
                     TYKNFCEAAKAGVSIQGEGRCGIL"
     gene            complement(11897..>12435)
                     /locus_tag="PKH54_01505"
     CDS             complement(11897..>12435)
                     /locus_tag="PKH54_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98591.1"
                     /translation="FRIDGKRAGQIFGGGLATVLEELKAQTGSWFYVPLLQEAGVIDE
                     TQARQLYTHALVWSFGHLSQGLFAPDGNPKPYSQLSAVIVGRLMDAGALEWVDVAGAD
                     GSRKGLFNMKFDLVPAVVKTLMRDVVSVKATGNVEAARAMVDDFVTGSKKALVHLDEI
                     KARLAPYPRGSMIYQVKM"
BASE COUNT         2588 a         3794 c         3725 g         2318 t
ORIGIN      
        1 aaccatccag cagcagtatc tgccgtccag gtggcccgac cggcgggtgc tcgggatggt
       61 aagaccgtcc gccgaagtgc tggccgacct tccccctggt gcgattcccg gaatcacaag
      121 gcccggaaac gcgacgggaa cggttgccat tcttgccact cccggaaccg tcttgtcacg
      181 ctctttcgtc atcgaactgg aaaagctggc ccccggcctg aaagtcatcc agcaggcatg
      241 cccgatgtgg gtgcccctgg ttgaaaacgg ggaactcgag ggtccgggct gcgaatactt
      301 cgtcaggaaa taccttgagc cggtcctgcg cggcccgcag attcccggca ggatactgct
      361 tgcctgcacg cactatccgc ttcttctgcc acgcatcagg gaagtggttc cgcccgacat
      421 cgaaatcctt acccagggcg gaatcgtttc ggccaggctt atggactggc tggacaggca
      481 tccggaacac gccggcaggc tgaccggaaa cgggacacgc gaatacctta cgaccgacga
      541 tacaaccagg ttcgacgaac ttgcctcgat gtttctttcg accccgatca tgtcgcggcg
      601 ggttcatctt ccaagactgg acacttgaac cggacaccgc gtttcctgca tgatcccgtc
      661 atccagatcc gggcggaacc gcgcgatgga actttttctt aaccacgcat gcaaccttcg
      721 gtgcacgtat tgttacaacc attcgcattc ggtcaacccg atgaccacag aggtgatgga
      781 tcgcgcgctt caaatggctt tcgcccaacc cgtcgatatc gtcaacatcg gcttcttcgg
      841 cggggaaccc ctgctgagcc cggatcttct ttcaaagggc gttgcgcagg cacggaagct
      901 ggcctcggcc ggacgacgga ggatgcgcct gataatgacg acgaacggca cgctcctgga
      961 cggcccccgg ctggatttca tcctgaagaa caggttcagg ctggcagtca gcctggacgg
     1021 cccggaacac gtgcacgacg cccacagggt ctttgcgtcc ggacggggat cccactccct
     1081 tgtcgttgcc aaccttaaac acgcgttgac cttgaagccg gacatatccg tgatttcggt
     1141 ttccgggccg gacaacatcc gacatgccgg cgccgcgctt cgtttttccg catcgctcgg
     1201 ggttcttcgg caccatccgg ccatcgatta cggggcctgc tgggatgaaa acgcgctcag
     1261 gcgctttgcc cgggggattc agggcctggc ggacgcatgg gccgacctgt acagggccgg
     1321 aaatccggtt tccgtgcccc tgatcgattc aaagatcgcg cgaaacataa tccatccaca
     1381 ggtcagcctg ccgcggtgcg gatgcggcgg gcctgaatgg accgtcggac cttccgggcg
     1441 catatacccg tgcgacagga tggtcgggcc tgatgacgga tcgggaccgt tcatcggtga
     1501 cgtcttttcc gggatcgacc cgcttttgca aagggaattc cttgactgtc accattcgac
     1561 gccagattgc tgtgccgggt gtgccgatgc gcaccgctgc tctttctggg gaagctgcat
     1621 caagttcaac atgaccgggc gcatcacatc gacaccgcct gcggaactgt gccggatgga
     1681 aaaggccatc atgcgggccg ccgacagggt tgccggtgaa ttgtggacgg aaggcaaccg
     1741 tgagttcatc aggcgcttct acgaggacga gcgggcacgg ctggcgatgt ccagatgcaa
     1801 cccttccgga gcctgaagga attttttcgc aggatgtcgg aggtcgttca ccaccgcgac
     1861 gaaccgtgct agattccgca ccaattcacc tcgacaggcg agtcatgcga aattccgtcg
     1921 cctttgtgat tgccctgctg gcggcaagcc acgccgttgc ccagccggcg ccgcaaccga
     1981 caaaacagcc ggcagccccg gcaccgtccc ctgacggcca gggcgtttac ggcctttact
     2041 gcagcggatt cgccgccgga agcgaaaaga tgttctcgga tatcgtcgaa atcatcgacc
     2101 ggacatacct gaacgccgtg gtgatcgacg tgaagggcga cagcggacga ctcagctaca
     2161 aatccgaggt tccgcttgcc aggaagtacg ggtccggcca ggcaaagata aaggatatca
     2221 ccgggttgat aaggacgctg aaggacagga acatcattgc gatcgcccgc atcgtggcct
     2281 tcaaggatca atggatggcc gaaaagaacc aggcatgggc cgtgcgcgac atcgacggtg
     2341 gcgtctggaa ggacaggaag ggtgcggcgt ggatcgaccc ttacaaccgg aagtactggg
     2401 attacctggt tgaaatcgcc gaggaggcgg ttcggcacgg gtttgtggag atccagttcg
     2461 actacgtccg cttcaccagc gacggcgaca catcaagatg cgtctatccg gcacgcacga
     2521 ggacgtcgaa gcaggatctg atcgcgcagt tcctggagta cgcatcaaga cgcctgcgac
     2581 cttccggggt gaaggtttcg gccgacatct tcggcctggc caccagcact cccggcgaca
     2641 tgggaatcgg gcagaacctt gaaaagatcg cgcttaacac ggacatcgtc tgcccgatgg
     2701 tatatccgtc ccattacggg gcgggcaacc tgggcattgc cgatcccgac aggaacccgt
     2761 acaggacggt tttcaggggg gtctcggacg ggatggcgcg acttgacgcg gccggtttga
     2821 aaaccatgat gcgtccctgg ctgcaggcct tcagcatcaa aaacaggtat ggaaaggccc
     2881 agattgccga tcaggttcgg gccaccattg atgccggtgc gcgtgaatgg cttttctgga
     2941 acgcgggctg cgtctataag ctcgaatggt ttcaggaacc gtcacttcga agatgacgcc
     3001 ttcatggaca gggtcaggac gaaactggcc accggttcct ggccatacct ggacgggact
     3061 gtcgccacaa ccctgaccgg aaggttttcc cgctgcccgg aaacggcaac ccgatccacg
     3121 aattcgcgaa tttcggcaac ctggtcggtg gtgaagtggg tgtcgccgtc ggcccgcttc
     3181 aggaactggg ccgaaaagtc cttgaaaacc aggctgatgc ggtatccgcg ctcctgcatg
     3241 cgtgcaagcg cgggcagcgc ggcggtcacg tcggccgcaa gcgcaagggc gccgaagtaa
     3301 agcgagccca gatggttcct ggacctgcgg cgcaaaggaa ccaccacgac caccctttcg
     3361 tcaccggcct cgacgatgcg cggcccgacg tgtccgacaa ggggaatctt gaagtgtccg
     3421 aaccaccaca tccacagccc gatcctgctt cttgcgggca gaaggtcaag caccctgttc
     3481 atgaacgcat ccatcttgtt ctccggataa cccgattacg gcgatcgttg cacccgaatc
     3541 gcccagcccc aagcacgttt tgcaaaaggc tttgaaatca atgaacattc aagcccagac
     3601 aaggttcggc aaggcgccgc cgtcctggat gacgaatcag ggcttcgggc ttgcccccga
     3661 ctccaccagc gccatgagat cccgcaccga aagggtgatg tcccgactgt ggcggaacag
     3721 gccaccacca atcaggaata tgacgtcatt cccgtaaaat cccatcgaat ccgagatgtt
     3781 ggaaaagttc aacccgccgc ccggggccgg aaacatcggc ttcaggcccg acatcggcat
     3841 cctgcatcca gacaggatcc cgacgcactg cttcctcgag aaggaaaagc gcccgccgaa
     3901 actcggaaat atgacggcgt cggcgcatgc aagccgaagg acctggccga aaaaggctcg
     3961 ttccgtgaag gcgggggatg gcccgacaac ataggaaccg gaaaacgccg ggtggaagaa
     4021 gaccggaagt ccgacttccg cactggtcgc caccgacctg agcatcgaca ggccggtcag
     4081 accgccggaa acgatgaccg cgccggcgcc ggcccgacgc gcgaatatgg cccgctcaag
     4141 ggttgcggga ccgtcagccg tcacgttggg gatataaaga catcggcgac cggtttcgcg
     4201 gttggccctg tcaaccgcct ccatgcatct ggaaaccctt tcgacgaaag gcgaaaagcg
     4261 ctggtcggca aggccgtgat cgtccttgat gaaatcgatt cccccaagag cgaatcgata
     4321 ggccatgtcg gccagggtat ccacgtcaag tcccatcggc ttgattgcgg aacacagaag
     4381 cggcctggaa aagactccga cagcctgcct gattccctcg atgccgaatc ccggaccacg
     4441 aaatatggcg gcctggaccg cggttggatt gatcgactcg acgcggactc cgggtttcat
     4501 gctcgaattg ccgaagacga cgttcatgaa ctgggaaaac tcggttccgg cggtcgcttc
     4561 atcgtatgaa acggcgataa ggtacctgcg catccccact ggcagactat agcccgggat
     4621 atccgacagg tcgccggtaa cagcctggta aagttcgacc gactcgacgc gacccgtaat
     4681 ttcgcctttg acgtaagtgt cggtgacaag atcgtaagga aactcgatcg tctgctcgac
     4741 ggccagggcg aaggcctggg cctcggcatc cccacggtcg gtggcaggca gggagtacct
     4801 gaccagaaat ctgcccattg ttacaacgcc tccgccttcg ccaccgaatg aaccggctcg
     4861 accctgacgg cgccaaggtc agccaatgaa atcgaaagat tcgcgccaat caggtcttga
     4921 acggcgttga ccagtgccat ggcgtcgaaa ccgtgttcac gcatcaaagc cgcccttccc
     4981 gcgccgtggg accacgtgtc ttgaagccca aggcggacaa ggggccttcc gaccccggtc
     5041 gacgcgcacc gcgaagccac ggccgtcccc agcccgccgt caaccagatg gttttcgacc
     5101 gtgacaatcc ccctggagcc agcacggatg gcctggtcga tcaggccaga agcaaacggc
     5161 ttcaaagtcg atatgtgtac gtgggtgatg gacacgcccc ttgccttcag gactgccgag
     5221 gcacgcagga cttcctcggt gcagatgccg gacgaaaata cggtcacctc atcgccaagc
     5281 gacaggatgc gtgggtggtc ggccctgatc ggctcgtcgg tcgggaaaag gcgcggcacg
     5341 tcaccgcgca gcatcctgac atacaccggg ccgtcaatcg aatatgccag gtcaagaacc
     5401 gactcgacct cggtcgcgtc ccccgtttcc agaaccgtca tgttcggaag ggaacgcatc
     5461 agcgccacat cgtcgatggc ctggtgagtc gcgccccccg gggtcgtgat tccgggaagg
     5521 aacccgaaca tcttcaccgg caggttcgga taggcgaccg acatcgcaac ctgatcgaat
     5581 gcacgccggc agatgaaaac cgcaaaggtg tgtaccaggg ggatgaaccc ctcgcggcac
     5641 aacccgccgg caacggaaag catgttctgt tcggcgatgc cgcaactgat gaaccgccgc
     5701 ggatatgcgt cgcggaagcc gtcggcctcg cagcttccgg taagatccgc ggaaagcann
     5761 nnnnnnnngc accaggacat cgggcttgtc ggccgcccat ttgacaaggt tgcgcccatg
     5821 cggacgactg acgatttcca tcaggacccc ctcttcggcc atgcatccag cacagccttg
     5881 tagccggcct tttcctgcgg cgtcctgaaa cgcaggtaat gcagtttcgg ggcgttctta
     5941 cgcatcagat caaggccgcg acacggatcc gtcatggcaa gcacgaaaag aggccgaccg
     6001 tcgggcgccc gcgagccggc cttcatgatc gcggccgggt catgtccgtc aaccacggcc
     6061 gccctggccc cgaaggatcg tatccggaca tcgatcggat cgatccgcat aactgaatcc
     6121 accgctccat cgcactgctg accgttgaca tccacgaaca cgaccatgtt gtccaggtgg
     6181 tggtgtgcca tgaactggat cgactcccag gtcatcccga tctggaactc gccatcggac
     6241 atcatgacaa ccacgcggcc ggacgcgccc ttcaacttcc gtccaagtgc catgccggcc
     6301 gcctgcgcca gcccctggcc aagggaaccg gtcatgactt ccatgcccgg ggaatgctcg
     6361 gcgccgatca tctcgaccga agaaccatcc cggttgaact gcttcatccc ctgcggcgac
     6421 atccggcccg tctcgaccag aagcgcataa agaacaagcg agtaatgcgt cggggaaagg
     6481 atgaagcggt cacgggcgtc gtgaacgccg ccattgaagt cggcgccggt tcgatactcc
     6541 gtattgccgt gtcggggcgt gccgagccac ggacgtggtt cgatcggcct gtcgggcatg
     6601 ccgatattca tgaaccggcc gtaaagagtg gcaagcaact cggcggacga gcacgcctgg
     6661 ctcatgtagc cgccatcgtt gtccagggta tgttcaagca cggcccgcct gacgcgatcc
     6721 gcaagcaagg ccacatccac tttccatccg ccagccatgt tcaccttccg cggcgtcccc
     6781 gatgatagtg ccacacacga aaatgcattg aaacgcagac ctgacgggcg gtcaacatcc
     6841 gaaaccgatt ttcctttggg acggcagggg cttttgttat tatccgtcgc tgcatcccga
     6901 acccaatcga ggtgaccatg agaacgaccg ccttaagaaa gcccggaaga cacgcccatt
     6961 tcaaccagat gacggcagcc gcgctggccg cgatcgcgct ggtgtcgttc ggccagccgc
     7021 aaagggccga cgcgtgcacg aacatccttg tcacgaaggg tgcgtcggcc gatggctcga
     7081 cgatgatttc gtattcggcc gactcccacg acctgtacgg attcctggac atcaagccgg
     7141 ctggaacgca tgaacctgga acgatgattg acatcatcga ctgggatacg ggcaagaaac
     7201 tcggccagat tccgcaggct ccggtcacct ggaccgtcgt cggcaacatc aaccagcacc
     7261 aggttgccat cggcgagaca accttcggcg gtcgcccgga acttgagggc ccggccgggc
     7321 tgatcgatta cggcagcctg atgtaccttg cccttcagcg ttcaactacc gccagggaag
     7381 ccatcaaggt gatgaccgat ctggtggcgg aatacggtta ccacagcacc ggcgagtcat
     7441 tttcgatttc ggatcccgac gaagtctgga tcatggaaat gattggcaag ggcaaggatc
     7501 gcaagggggc ggtatgggtc gcccggaaga tcccggacgg atacgtttcc gcgcatgcaa
     7561 accaggccag gatccgccag ttcccgcagg atgatccgga aaacacgatt ttcagtcccg
     7621 acgtcatttc ctttgcacgg gaaatgggat ggttctgcgg cgatgaccgg gacttctcgt
     7681 tcgcggacgc gtacgcgcca ccggattacg gcgcccttcg cttctgcgag gcccgcgtct
     7741 ggaacgtctt caaccacatc cgaccttcgg aaaagattcc ggccgacttc gtgaaggcgg
     7801 tgccgggcgc aagtccgatg ccgctgtgga tcaagcccga caggcccgtc tcggtcaagg
     7861 acgtgatgac ggaaatgcgc gaccacttcg agggaaccga attcgacctg agcaggggtg
     7921 tcggggccgg cccattcggg ctgccgtacc gctggcgccc gcttcagttc aaggtcgggg
     7981 aaaaggagta cttcaacgaa aggtctattg ccacgcagca gaccggcttc tcgttcgtcg
     8041 cccagtgccg cgggtggctt cccgacccgg tcggtggcat cctgtggttc ggcgtcgatg
     8101 atacggcatc gtcagtctat gtgccgatgt acgcgggaat caccgcggca ccacgcggct
     8161 tcgggcgcga atccggaaac ttcaacgaat tctcgtggga ctcggcgttc tgggtgttca
     8221 gcgcggttgc cgaaaaggcg tattcgcgat actcggacat catcaaggac atccgcaccg
     8281 tccagacgga acttgaatct tcgtttcact cgatggtgcc ccaggtcgat gaaacggccg
     8341 taaggcttca cggtgcttcg cccgaactgg cccgcgcatg gttgaccgcc ttttccgcaa
     8401 cggtggggga cgtcaccatg aaacggtggc gggatctgtg gggccggatc atggtcaagt
     8461 acaacgacgg aaacgtcagg aacgacaagg gtgaaatcga gcatccgccg ctgccggacg
     8521 aatggtacag gcggatcatc caggaaaggc cgggcttctt cgaggtcctt ccggtcgagg
     8581 cggccaaggg cccctcgtgc cgctgaatgc cggggtgaac aatgcaggaa acaacgtcgg
     8641 gtaatcgatt tccaagatcc ttctggaccg ccaacgtgac cgagcttttc gagcgcggcg
     8701 cgtactatgc tatggcgagc ttcgtggtca tctacctggg ccagctcggc ctgggcgact
     8761 actggccaag cacgctcaac ggcatcctct ggaccatcat ctacttcctg ccgattcttt
     8821 cggggacgat cgcggaccag gtcggcttca ggaagtcgct tctgctggca ttcgtgctgg
     8881 ttgccgccgg ttacgcgtgc atgagttacc cggttctgat tgccgggcac gaactttccg
     8941 atatcgtgaa gcctgaagtc acggcatcca tggacaccat agtcgcgatt atcgccggga
     9001 ttgtccttat cggaatcggg ggctcgttcg tgaagccctg catttccggg acggtccaga
     9061 agacctcggt cggccgcgcc acgctgggat tcgcgatatt ctacatggtc atcaacatcg
     9121 gcagcctgtt cgggcgcggt gcctcctaca tcgtccggac aaaattcaac aacatcagcc
     9181 tgatcttcgt ggttgccgcc gcgtgcgcca tcctggcgct gctggttgtc gctttccttt
     9241 acagggatcc tgaaagcgag accgggactg ggaatgccgc caagccccgc cgttcaatcg
     9301 gggaaatcct tctgaacatg atccttgtgc tgaagaacgt gcgcttcgcg ctgttcctgc
     9361 ttgtctcgtc cggtttctac ttcctttacg cccaggtcta taacgtgctt ccgctttacc
     9421 tgaagaaggt cgtggaaaca aacccggccg tcgatatcta tacgatggcc aacccgatcg
     9481 tgatcgttct gttccagctt ctgatcacgc gtttgttcgg aaagatgaag ccgataaggt
     9541 cgattgtcat tggaacggta atcatcggcc tgtcgatggc aatcaacctg gttccggtgt
     9601 ttacgtcggg tgtgcgcgtc caggtgctcg acctgctgcc cttcggatcg gctatgatcg
     9661 tcctgacggt tgccatgatt gcgtttggcg aactcttcac gtccgccaga acctatgaat
     9721 acatcggcgc gctggctcca aagggccagg aaggcctgtt ccttggatac gccaacctgc
     9781 cgatggcaat cggcgccctg atcggcggcc ccgccggtgc tgcaatcttc aacgaaatca
     9841 tggcgaggga cgcaaaggtt ctcgacaacg ggctgcttga gctgaatccg gtaaacaacg
     9901 ccatcgggtg ggttgtcctg atgggaatcg gaatcctttc cgcgctgagc atgtggcttt
     9961 acaaccggtg gctgatcagg gctaatgcgg cggcggaaaa cgcctgaaaa ggcgacgact
    10021 tgcatacacg acccaatctg atgtaaaatt ggcgactgtc gaatcagggt tatggcgtcc
    10081 ggttatggcc gccgatggag gatagtatga accagatgcg attcatgttc ctgtttgcgg
    10141 ccattttcgg actcgttgtc ggttgcgggg gcggcgacaa ggttccgacg ggcgatcttg
    10201 ggccggcgga cacgattcag tcggatacgg cgccggatac catcgtgccc gaggatgttc
    10261 cggttggtga tgtccagaac cacgacacca acgtgccgga cgtaccggtg ggcgacgtac
    10321 ccgtgggcga tgtgccggtg ggcgatgtgc cggtgggcga cgtgcccgtg ggcgacgtgc
    10381 ccgtgggcga cgtgccgaca gatgaaaaca ccccggttga caatggcggc gacacggtca
    10441 ccgactcggt tggcccggtt gcctgcacca cgaacgccga atgcacggaa gaacagttct
    10501 gcaaggtcaa cacctgcggc gaagccggtc tgtgcgaacc gacgccggcc ctgtgcgtgg
    10561 ttccgctgat atccgaaaac ctctgcgcgt gcgatggact ttattacgag tccacctgct
    10621 tcgcgaacat ggcccgcacg aatgtcgcaa acggcgcaag ctgcggttcg gaaacgacgt
    10681 gcagcgacaa ttcggggtgc caggaactgg aatactgcct caagtcaacc tgtggtgcgg
    10741 cagagggatc gtgctcgcta aagcctcaga tgtgcccgat cactcccaat ccggtatgcg
    10801 gatgcgatgg cgtcacgtat aacaatgcct gcaccgcagc cgcatccggc gcaaacatcg
    10861 actcaaccgg ggccgcgtgc gcaaccgtga cgacgtgcac cagcgacgcg gactgcacgg
    10921 tcggtttctg cttcaaatac agttgcgagg ccgctgccga aggcacctgc gagaccaagc
    10981 cgccgttctg cctggtcatc ggcgatggtg tctgcggttg cgacggcaac agctatacga
    11041 acgaatgcct tgcaaagaag gccggcgttt cggtcgatcc gaccggggac gcgtgcgacg
    11101 cgaccgaaac gtgtgcgtcc aacgcggact gcacgtcaac acagtactgc aaaaagacaa
    11161 cctgcggcgc cgccgagggc gtgtgcaccg ccgtgtcgat caagccgtgc agcgacctgg
    11221 gagatgcggt ctgcggatgt gacggcgtcg tttatccgaa cgcctgcaag gccgccaagt
    11281 gccgcacgaa tacccagaaa atcgccgcct gcaccagcac ggacacatgc tccgtgaagg
    11341 atacctgcct gcgtggtgac tactgcctgt ccgcgaattg cggaagcggc gtctgcatgc
    11401 cggttccgaa ggcctgcgaa accgccacgt accaggtttg cggctgcgac gacatcacct
    11461 acaagaactt ctgcgaagcg gccaaggccg gtgtcagcat ccagggcgag ggccgctgcg
    11521 gcatcctctg attgtcccga cctgaaaccc atcatcaacg ttgactacgc cttcgctgaa
    11581 gcatcaacgt gattgcaggc gggcgaattt tcattcgccc gtacggatcg tggggcggat
    11641 cgggccggac cataagtgct ccggtgaggc ctctcgaacc ggacacttca tggccggcca
    11701 caggcacgac tgcgtcactc ttcgagcgta cggttgctcc ccaggcgggc gaattttcat
    11761 tcgcccgtac ggatcgggca ggaccgcgac cggcgattga taacggcggc gggcgaattt
    11821 tcattcgccc ttacggattg ggcagaatcg cgacgggcgc ctgatcacga aggcgggcca
    11881 cggggacggg aattgattac atcttaacct ggtagatcat cgatcccctg ggatacgggg
    11941 caaggcgggc cttaatctcg tcaaggtgga caagcgcctt cttcgaacca gtcacgaaat
    12001 cgtcaaccat ggccctggcg gcttccacat tgccggtggc cttgaccgaa acgacatcgc
    12061 gcatcagggt cttcacgacc gccggaacaa gatcgaactt catgttgaac agtcccttgc
    12121 gcgaaccgtc cgcaccggcc acatcgaccc attccagggc gccggcgtcc atcagcctgc
    12181 cgacgattac ggcggaaagc tggctgtaag gcttcgggtt gccgtcaggc gcaaacaggc
    12241 cctgcgacag atgtccgaat gaccagacca gcgcgtgggt gtaaagctgc cttgcctggg
    12301 tctcgtcaat cacgccggct tcctgaagca gcggcacata gaaccacgag ccggtctgcg
    12361 ccttcagttc ctccagaaca gttgccaggc cgccgccgaa gatctggccc gcacgcttgc
    12421 cgtcgatgcg gaagt
//
LOCUS       DAOPPA010000025        12431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5909_length_12431_cov_4.356335, whole genome shotgun sequence.
ACCESSION   DAOPPA010000025 DAOPPA010000000
VERSION     DAOPPA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5909_length_12431_cov_4.356335"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKH54_01510"
     CDS             complement(<1..976)
                     /locus_tag="PKH54_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98592.1"
                     /translation="MKHGQKVFGCIFAPAMPLQAMVRTEPDLRELPLAAADGDRGGSR
                     ITHISASAGRLGVKVGMTASQARSIAADVVVRQPAAGVVAAAVRALTDIACAFSATVQ
                     VESDGRILFEAAGHEAIHGSFLEMGQRIINAAGNDGFIVRVGFGCGIRLAGIASRCGA
                     ATTVLETGAVAGVIAGMPVSILEPSPAMLEALTRLRVITVGDLAALPARGIGSRLGSE
                     GTRLHALASGIDESVITKPVVPDDRFIECVKLDWTLDNLEPLIFLIKGAADRISARMQ
                     MRGLVPGKAGLTLDLENSGIHFLETAAGGISSSSSTISSVLHRVLSRNP"
     gene            complement(973..1674)
                     /locus_tag="PKH54_01515"
     CDS             complement(973..1674)
                     /locus_tag="PKH54_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98593.1"
                     /translation="MPGNSAIARLLERMPAGLVRRASEQVRSNPVTPTGISALDAALG
                     GGWACGRLNMLVPGPSASTGRTTVAMAAVCAVTSSGLPAAWIDGDGSMDPPSLLACGA
                     NTKLLLWVRGPMSADALLKSTHEVIISGMFGLIVVRPPLDGWYGGGAMHWLRLSREAE
                     LSGAPVIVLASGSATSAGNSSATFFAALDADWSGPIGAGCLLMGGTLRLETDLSDGHQ
                     AECTFRSNAPWLVFQ"
     gene            1834..3069
                     /locus_tag="PKH54_01520"
     CDS             1834..3069
                     /locus_tag="PKH54_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOC98594.1"
                     /translation="MEFHLRAMFRAAVESAMPARVLPPLLEGVSGPVWLTGAGKASAG
                     MAAAAAPMLDIRQGAVVTLRDAPVPGDIAGIDVMFASHPLPDESSREAAERMLGIARA
                     AAAVPGATLICLLSGGGSALMSLPPSGVTGAVKAGLIRDMLAAGSPIQDINCVRRHLS
                     RIKGGRLAAAAFPARVMTFAISDVVGDAPADIASGPSVPDPTSLAQAAHALARSGIRP
                     HRALIEALSGPDAETPFPGDSRIGDCRFFLAASGSTALAAAAAVAPLPDVVNLGAGII
                     GDSRAAAAEHARLVLELRSTGRRCVILSGGETTTVIRGPGRGGSNTEFLLALAAELQG
                     VRDIWAIAADTDGIDGRGGHAGAWFGPDLVMEPGLADKAAEFLAQSDSGSFFDQAGLL
                     VRTGPTGTNVNDFRAIIVG"
     gene            complement(3083..4000)
                     /locus_tag="PKH54_01525"
     CDS             complement(3083..4000)
                     /locus_tag="PKH54_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOC98595.1"
                     /translation="MPDIHDDFINGLFEKARTNPGRVAVPAADNAEALEGIAMAMKDG
                     VLEGGILIGEEDSIRKVAEEVSLELDRFEIVAEPDWDLAADLAVRSIVEGRAGFLLKG
                     KLGTSNYLKAVLNRKYGLVPEGRTLSHVAMAMIPTYHKPLFFGDAAITIDPDVQTKAS
                     IIRNIVDVNHRIGIANPLVALIAAVEKVNPKMKSTTDAAALVDMAAAGAFPGCVVEGP
                     YDLYIAISSEAAEIKGVSGKVCGNADVLVFPEINSGNVFYKSLKFVPDSWTAAVVGGA
                     SIPILLPSRADSAMTKRMSIVLAAAMTAA"
     gene            complement(4032..6707)
                     /locus_tag="PKH54_01530"
     CDS             complement(4032..6707)
                     /locus_tag="PKH54_01530"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOC98596.1"
                     /translation="MRLFLISAAMATIIAAGCGGADDSNDLSGMDSGQQDSFPDEGGG
                     EIPVTDIGGNDLVDPDQGGVDDVSTDAADAGAQPDLTPFPDGATARRIESPDDLVTGP
                     MSSGTIGDILLSNKHVRFVIRDQQIGLYSPSAGSIVDADVTRDPGDGGHEKFLELFPM
                     IGFGRVLMADKVEIIDDGTFSGTARVRISGRDHGMPIIDMLVPTFPFNLQAVMDYVLE
                     PDAGELRIELAVTNGAKYEQDVEIGTVVQFGRRLEYFYHQCGRDKSCLIGRDDVEWMA
                     AGSDDVSYAFTTAPGTGMSVVLAEEQLLLTRTAKTSLAPDATTSSVQYLIVGSGTIDE
                     VSTRASDIRQTATLRTVTGKILLTDSISDVRDARVFARKQGASGKLGWMAAGQPDEEG
                     NVTIHLPDGVWDLTTTLPGSGDVTSAVAVAEKDDNTFEIHAVAAGRLHVTINDGDGGL
                     LPAAVFIKNGLDMPPAAGGEFHAVRGGDDNFMLLPGEYTVMAMKGLEYGLVSANATVL
                     AGAPTHVMLSLHREVETPGQVTVVSHAHAENSIDSYVPMNDRVHNALAAGIDFPMITE
                     HDFFSNLQPFIESEGYQDRLKSTVGIEISPLGFHTVGLNCSAPPSYPTYFAIRFGDYD
                     SDGYLSNQYTPGQIVEKTRGFGCQYVGAAHPWEGSAMFNYIGMGPEDDPADFAHLLDL
                     HDLDGMELINSDDNWSDLDTLHLPAWFRMLGLGYRITAVGGSDEHGYSIRYGHPLNLV
                     QASSDNAADLEPTDVFEAIREGRTILYGGPYIDLDMGGFGVGDVVDMSVAEPPVLRIR
                     LDAPSWMTIDFVRIYVGGVLVNEFVLTPEMAAGDWLLDTTFEPEITGDSYIVVAAGSS
                     REEARMPGYGRFPVTVANPIWLDSDGNGWEAPISE"
     gene            complement(6849..7490)
                     /locus_tag="PKH54_01535"
     CDS             complement(6849..7490)
                     /locus_tag="PKH54_01535"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOC98597.1"
                     /translation="MEEKVYGFPKLNEPAPDFLAPTTHGPKRLADYKGRWLVLFSHPA
                     DFTPVCTTEFIAFAKAHEQFLSMNTDLLGLSIDSVHSHIAWVRAIKDKFGVDIKFPII
                     ADLSMEVAAKYGMIQPGASNTSAVRAVFIIDDKGILRAMVYYPMSNGRQIDEFIRLVQ
                     ALQTSDKNGVATPENWRPGEKVIVPPPGTTEAAEKRLNEGYECIDWYFCRKSL"
     gene            complement(7648..8880)
                     /locus_tag="PKH54_01540"
     CDS             complement(7648..8880)
                     /locus_tag="PKH54_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOC98598.1"
                     /translation="MSEEEVFAAEDFEAMLNESFPSGDGFNYFQVGDKVRGTVVSVGD
                     GTLLVDIGQRSEASIAMEDFTPEELAAMKVGDVIEANVVKFAGGATVLSRSIGGRGAD
                     MSAIRMAKEADLPIEAKVTGHNKGGFTVEIGPVRGFVPISQMEIGPALAPEQYIGRVF
                     RFKVIEAREHEAVLSRASLLRQEMAEERGRILSSLAVGQTVAATVVKVEKFGVFVDIG
                     GGVHALVPVSELGWHGRDEILGGMEPGRELNVEISRIEQKDGKPRISASLRSRQDDPW
                     NSIDEVAREGMTVAGKVTRLMNFGAFVEIAPGIEGLVHVSEIAAGRRVRHPGDVLSIG
                     QRVEASVRKIDHAARRIALSIKALAPVPEMTVPEVDPDAAENAAIIARYSPSAPKQED
                     APKGETAFSLALKKARLK"
     gene            9105..9749
                     /locus_tag="PKH54_01545"
     CDS             9105..9749
                     /locus_tag="PKH54_01545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC98599.1"
                     /translation="MKLMISMQMFAVVMFVAVSGCQADDRGQGSGGAAVAGKAAKAAP
                     APVPVAPVKVSIQTSVSTGGGLIEMELYPDKAPKTVENFVKLARQGFYDGVLFHRVVP
                     GFVIQGGDPLTKDPAMKSRWGSGGPDYRFEDEPVKGDYVRGAVAMANAGPDTNGSQFF
                     ICVNDLTGRLPKQYNWFGKVTRGMDVVDAIVAMPRDERDCPNENVVMQKVTVTD"
     gene            complement(9754..10245)
                     /locus_tag="PKH54_01550"
     CDS             complement(9754..10245)
                     /locus_tag="PKH54_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOC98600.1"
                     /translation="MISEACRIHVARAACIVATLFAGCASGTHDCGIWGGRVTAVLDG
                     DTVVIDDSWTVRLLGIDAPETGCCWSSRSRAAADALLMDKYVELEFTSRCTDDYGRLL
                     AHLIVEGRCASEYLASRGHACAWILQEDDGRAEEIRIAARRAASIGLGFWGACAEVSC
                     RSR"
     gene            complement(10242..11411)
                     /locus_tag="PKH54_01555"
     CDS             complement(10242..11411)
                     /locus_tag="PKH54_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOC98601.1"
                     /translation="MMKRRIDRFVCIAIAVAGTVLHPGCGQTATPVRDVVTGCTCTGG
                     ELVITELLINPIGEDAGLEFIEVANVSAGPVELDGLLVVSGSESRPRISGVTGWTKPS
                     IPAGGRVFFSEAPPGGNPAAATLDSLTLPNESGTVTILCNDTEVASFSWGTGVPPPPE
                     GFSLQMEPVPQAGTPAAWCASTAAPDTEGNSGTPGETNLGCPVPAGAECRNPATGTYR
                     RPVRPTLDDLAVTEVFFNPKGSDTLKDEWLEIIALADFDLNGISIVHFNGPGRDHASR
                     AFSIVSETCLAVRLGEVIILGGPDGASLEGTDTFYNATGGSAMLEFSDTSGNMIATAI
                     HPKVPEAKSVHLAATEDVATIASRDDDAAAWVVTGCDGGTPGRLAASCTTRKKKR"
     gene            complement(11408..>12431)
                     /locus_tag="PKH54_01560"
     CDS             complement(11408..>12431)
                     /locus_tag="PKH54_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98602.1"
                     /translation="RFGAVGVAGHARLPLNGDLAAEFAVSDNGATAGMLETAFSPVSG
                     LDLEPSIRYYGRNWDNPWTGSTADSREFRGNRARDEVAGRLDVTWRSSARTINRFRID
                     VGRNGHSDLAESRPQVALEAATALQVFASRHERLGIEISFRDRDLAVSGRGLSWDQYY
                     SATTGNLSGGCRVDWSVWFQTKRIRRMALAIRVGQAFRDAATLRDRFDMDLSATLKID
                     ANLSPGPLLSAFARFSDESMAADTSVTTGQSCLDREIENRVPGICRGDSRVETGLTIA
                     QDARFSTGLLRIRAIGRYTRHVDQRPSWGSASGFPGRNEIAAAISFDLLFTGRTGQAR
                     PDKGVR"
BASE COUNT         2266 a         3845 c         3778 g         2542 t
ORIGIN      
        1 gcgggttcct ggaaaggacc cggtgaagta cggatgagat ggttgaagaa ctcgatgaaa
       61 tgccgccggc cgccgtttcc aggaaatgga ttcctgagtt ttccaggtcc agcgtaaggc
      121 cggccttgcc cggcacgagt ccgcgcatct gcattcttgc cgagattcga tcggcagcac
      181 ctttgatcag gaatatcagc ggctcgaggt tgtccagggt ccagtcgagt ttcacgcatt
      241 cgatgaagcg gtcgtcgggc accactggct ttgttatgac cgattcgtcg atgccggacg
      301 ccagcgcgtg aagtcttgta ccctcgcttc caagtctgga accgattccg cgggccggga
      361 gcgcggcaag gtcgccaacc gttatgaccc ggaggcgggt cagtgcttca agcatggccg
      421 gggatggctc aaggattgac acgggcatcc cggcgatgac tccggcgacc gcgccagttt
      481 ccaggacggt cgtggccgcc ccgcatcttg acgcgatgcc ggcaagcctg atgccgcagc
      541 cgaatccgac acggacaatg aatccgtcgt ttccggcagc gttgatgatc cgttgtccca
      601 tttccaggaa agaaccgtgg attgcctcgt gtccggccgc ctcgaaaagg atcctgccgt
      661 cggattcgac ctggacggtt gctgaaaatg cacaggcaat gtccgtcagc gccctgacgg
      721 ccgccgcgac aacccccgcc gccggctggc ggacgacgac atcggccgcg attgaccgcg
      781 cctgggatgc agtcatgcca acctttacac cgagtcgtcc ggccgatgcc gatatatggg
      841 ttatccttga accgcccctg tccccatccg ctgccgccag gggcagttcc cgaaggtcgg
      901 gttccgtcct taccatggcc tgcagcggca ttgcgggggc gaatatgcat ccgaacactt
      961 tttgaccgtg tttcattgga acaccagcca tggagcattt gacctgaacg tgcattcagc
     1021 ctggtggccg tccgaaagat ccgtttcgag ccgcaatgtg ccacccataa gaaggcatcc
     1081 cgccccgatc gggcccgacc agtcagcatc gagcgcggcg aagaaggtgg cggaagagtt
     1141 gccggcagat gtggcggaac cactggcaag cacgattacc ggggcgccgg aaagttcggc
     1201 ttcgcgggac aggcgcagcc agtgcatcgc gccgccgcca taccatccgt ccaggggcgg
     1261 acgtacgaca atcaggccga acatgccgga aatgatgact tcgtgcgtcg atttgaggag
     1321 ggcatcggcc gacatcggtc ccctgaccca tagcagcagt tttgtgttcg cgccgcaggc
     1381 aaggagggac ggcggatcca tcgagccgtc accgtcgatc caggccgccg gcaatccgga
     1441 cgatgtaacg gcacaaaccg cggccatggc gacggtggtg cgtccggtcg atgccgaagg
     1501 gccgggaacc agcatgttca gtctgccgca ggcccatccg ccgccaaggg ccgcgtccag
     1561 tgcggagatt ccggtcggcg tgaccggatt gcttcgcacc tgttcggagg cccgccttac
     1621 aaggccggcc ggcatccgtt caagcagtct ggcaatggcg ctgtttcctg gcactttctg
     1681 atgccgtaat ggtccttccg tcaattatac tgaacgtttg ttcaggaagt caaatgtgtt
     1741 cactgccgac gaaacgatgt tccaacgtcg gtcttgccgt cagataatgg taaccggtgt
     1801 tttcacgcgg ttccgaaccg gcaaggtggc gaaatggaat ttcatcttcg ggcaatgttc
     1861 agggcggcgg tggaatccgc catgccggca cgggtacttc cgccgttgct tgaaggcgtg
     1921 tccggtccgg tctggctgac cggtgccgga aaggcgtccg ccggcatggc cgccgccgcc
     1981 gccccgatgc ttgatatcag gcagggcgcc gtcgtgacgt tacgagatgc gcctgtacct
     2041 ggagatatcg ccggcatcga tgtcatgttc gcatcacatc ctcttcccga cgagtcgtcc
     2101 cgggaagcgg cggaacgcat gctcgggata gcacgggccg ccgcggcggt tccgggagcc
     2161 acgctgatct gcctgctgtc tggcggcgga tccgcgctga tgtcgcttcc gccatccggc
     2221 gtcaccggcg cggtcaaggc cggcctgata cgcgacatgc tggcggccgg ctccccgatt
     2281 caggatatca actgcgtaag gcggcatctg agccggataa agggaggtcg tcttgccgcg
     2341 gccgcatttc cggccagggt aatgaccttt gccataagcg atgtcgtcgg ggatgcgccg
     2401 gccgacatcg cgtcgggacc gtcggttccc gatccgacca gccttgccca ggccgcgcat
     2461 gcgctggcca gatccgggat tcggccacac cgggcgttga ttgaagcgct ttccgggccg
     2521 gatgccgaaa caccattccc gggggattcc cgcattggcg attgccgatt ctttctggct
     2581 gccagcggat ccacggctct tgccgccgcg gcggcggtcg cgccgttgcc cgatgtcgtc
     2641 aacctcggtg cgggcattat cggggattcc cgcgccgcgg cggctgaaca cgcccggctg
     2701 gtgctcgagc ttcgttccac cgggcgcagg tgtgtcattc tttcgggcgg cgagacgacg
     2761 acggtgattc gcggtccggg ccgcggaggt tccaacaccg agttcctgct ggcccttgcg
     2821 gccgagcttc agggtgtacg cgacatctgg gccatcgcgg ccgacaccga cggcatcgac
     2881 gggaggggcg ggcatgccgg agcctggttc gggcctgacc tggtcatgga gccaggcctt
     2941 gcggacaagg ccgccgaatt cctggcgcaa agcgattccg gttcgttctt cgaccaggcc
     3001 ggcctgcttg tcagaaccgg tcccaccggc acgaacgtca acgatttcag ggcgatcatt
     3061 gtcggatgaa ctgcttgcgc ggtcaggcgg ctgtcatcgc cgccgccagg acgatgctca
     3121 tacgcttggt catggccgag tcggccctgc tgggaagaag tatcgggatt gacgcgcctc
     3181 cgaccaccgc ggcggtccag gaatcgggaa cgaacttcag ggatttgtag aagacgtttc
     3241 cgctgttgat ttcggggaag acgagaacgt ccgcattgcc gcagaccttg ccggacactc
     3301 ccttgatttc cgccgcctcg ctggaaatcg cgatgtacag gtcgtacggt ccctcgacca
     3361 cgcatcccgg gaacgcgccg gcggccgcca tatccacaag ggcagcggcg tcggtcgtgc
     3421 tcttcatctt tggattgacc ttttcgactg ccgcaatcag ggcaaccaga gggtttgcga
     3481 tcccgatcct gtgattcacg tcaacgatat tcctgatgat gctggccttc gtctgcacgt
     3541 ccgggtcgat cgtgattgcg gcgtccccga agaacaaagg cttgtggtat gtcggaatca
     3601 tcgccatggc cacgtgggaa agggtccttc cctccggaac cagtccgtac ttcctgttca
     3661 ggacggcctt caggtaattg gaagttccca gctttccctt caggaggaat cccgcccgtc
     3721 cttcgactat tgaccgcacc gccagatccg cggccagatc ccagtcgggc tcggcgacga
     3781 tctcgaagcg atccagttca agcgagacct cttcggcgac cttcctgatg gaatcttcct
     3841 cgccaatcag gattcctcct tcaagcacgc cgtccttcat cgccattgcg atgccctcga
     3901 gcgcttcggc gttgtcggcc gccggcacag cgaccctgcc gggattggtt ctggcctttt
     3961 caaaaagccc gttgatgaaa tcgtcatgga tgtcaggcat ctttatcccc ttcgtcaaga
     4021 tacggtgttt ttcattccga aatcggagct tcccatccgt ttccgtccga gtcaagccag
     4081 atcgggttgg cgacggtgac cggaaatctg ccgtaaccgg gcatcctggc ttcttccctt
     4141 gaagagccgg cagcgacgac gatgtagctg tcgccggtga tttccggttc gaacgtggtg
     4201 tccaggagcc agtcgccggc ggccatttcc ggagtcagga cgaattcatt cacaaggacg
     4261 ccgccaacat aaatgcggac aaagtcgatg gtcatccagg atggggcatc aagccggatt
     4321 cgaagaaccg gtggttcggc cacggacata tctaccacgt cgcctacgcc gaagcctccc
     4381 atgtccagat cgatgtacgg gccgccgtac aggattgtcc gtccttcccg gattgcctcg
     4441 aaaacgtccg tcggttccag gtcggccgca ttgtccgacg aagcctggac caggttcagc
     4501 gggtggccgt accttatgct gtaaccatgt tcgtcgctgc cgcccaccgc ggtgatgcgg
     4561 tatccgaggc caagcattct gaaccacgct ggaaggtgaa gcgtatcaag atcgctccag
     4621 ttgtcgtccg aattgatcag ctccattccg tcaaggtcgt gaaggtcaag cagatgggcg
     4681 aagtcggccg ggtcgtcttc aggacccatg ccgatgtaat tgaacattgc gctgccttcc
     4741 cagggatggg ccgcgccaac gtactggcag ccgaatccac gcgtcttttc aacgatctgg
     4801 cccggagtgt actggtttga caggtatccg tcagaatcgt agtcgccgaa tctgatggcg
     4861 aagtacgtcg ggtacgacgg cggcgcgctg cagttaagtc cgaccgtgtg gaaaccgaga
     4921 ggcgaaatct cgattccgac cgtggacttc aacctgtcct gatagccttc ggattcgatg
     4981 aacggctgca gattgctgaa gaagtcgtgc tcggtgatca tcgggaagtc aattccggcc
     5041 gccagcgcgt tatgaacccg atcattcatg ggcacgtacg aatcgatcga gttttcggca
     5101 tgggcgtgtg atacgacggt cacctggccg ggagtctcga cttcgcgatg aagcgacagc
     5161 atcacatgcg tgggtgcacc cgccagtacc gttgcattgg ccgacaccag accgtattca
     5221 aggcccttca tcgccattac cgtgtattcg ccgggcagca gcatgaagtt gtcgtccccg
     5281 cccctgacgg cgtggaattc cccgccggcc gccggaggca tgtcgaggcc gttcttgatg
     5341 aatacggcgg caggcagaag accgccatcg ccgtcgttaa tcgtgacatg caggcgccct
     5401 gccgccaccg cgtggatttc aaacgtgttg tcgtcctttt cggccacggc aacggccgac
     5461 gtcacgtccc cggaaccggg aagcgtcgtc gtgagatccc acaccccgtc cggcaggtga
     5521 atggtgacgt ttccttcctc gtcgggctgg cctgcggcca tccagcccag tttgcctgac
     5581 gctccctgct ttcgcgcgaa gaccctcgcg tcgcgaacgt ccgaaatgct gtcggtaagc
     5641 agtatcttgc ccgtgacggt tcttaacgtt gcggtctgcc tgatatcgga tgcacgcgtt
     5701 gaaacttcgt cgatggttcc ggatccgaca atcaggtact gcacgctgga tgtcgtggcg
     5761 tccggggcca gtgatgtttt tgccgtcctt gtcagcagga gctgttcttc cgccagaacc
     5821 acggacatcc ccgtgcccgg ggctgtcgtg aaagcgtacg acacatcgtc ggaacctgcc
     5881 gccatccatt ccacgtcgtc ccgcccgatc agacatgact tgtcgcggcc gcactggtga
     5941 tagaagtact ccagcctgcg gccgaattgt acgaccgtgc cgatttcgac gtcctgctcg
     6001 tatttggccc cgttcgttac cgcgagttca atccgaagtt cgccggcgtc gggctccagt
     6061 acataatcca taactgcctg gaggttgaac gggaatgtcg gaaccagcat gtcgatgatg
     6121 ggcattccgt gatcgcggcc cgaaatccgt accctggcgg tgccggagaa cgtgccgtca
     6181 tcgattattt cgaccttgtc ggccatcaac acccttccga agccgatcat cgggaagagt
     6241 tccaggaact tctcgtgtcc accgtcgccg ggatccctcg tgacgtctgc atcgactatt
     6301 gaaccggccg aaggactgta caggccgatc tgctgatccc ggatgacgaa cctgacgtgt
     6361 ttgttcgaca ggaggatgtc gccgattgtc ccggaagaca ttggcccggt gacaaggtca
     6421 tccggggatt cgatccggcg tgcggtcgcg ccgtcaggaa acggcgtcag gtccggctgc
     6481 gcgccagcgt cggccgcatc ggttgaaacg tcatcgacac cgccttgatc cgggtcaacc
     6541 agatcgtttc cgccgatgtc cgttacggga atctcgccgc cgccttcgtc aggaaatgaa
     6601 tcctgctggc ctgaatccat gccggaaagg tcgttcgagt cgtcggcccc gccgcatccc
     6661 gcggcgatga tggtcgccat ggcggccgaa atcaggaaaa ggcgcatgtc atgcctccag
     6721 gattggattg tcgagggtcg tccatgccag tccagggaag ggccgccacg ggagtccgga
     6781 tgatatcaga atttcaccag ggattcacag ccgcgccaga gcccgtctgg aaaaggattt
     6841 ggatggcgct agaggctctt tcggcagaag taccagtcga tgcattcgta tccttcgttc
     6901 agacgctttt cggcggcttc agtggttccg ggtggcggaa cgatgacctt ttcgccggga
     6961 cgccagtttt cgggagtcgc gacgccgttt ttgtcggaag tctgcagcgc ctgaaccagg
     7021 cggatgaatt catcgatctg gcgaccgttc gacatcgggt aatacaccat cgcccgaagg
     7081 attcccttgt cgtcgatgat gaagacggcc ctgacggcag aggtgttgga tgcgccaggc
     7141 tggatcattc cgtacttcgc ggccacttcc atcgaaaggt cggcaatgat cgggaatttg
     7201 atatcgacgc cgaacttgtc cttgatggcc cgaacccagg caatgtgcga atgcacgctg
     7261 tcgatcgaca ggccgagaag atccgtgttc atcgacagga actgctcatg cgccttcgcg
     7321 aaggcgatga attccgtggt gcacaccggc gtgaagtccg ccggatggga aaacagcaca
     7381 agccatctgc ccttgtaatc ggcaagcctc ttcgggccgt gggtggtggg cgccaggaag
     7441 tccggggccg gttcattcaa cttcgggaat ccataaacct tttcttccat ctgattctct
     7501 ccttcggctt tgtgccgttt ccaaactgtt ctggtatgac ccggcatggc gggcgttgtc
     7561 aagaatgatc gcccgtccgg cctggaatgt aaggttttca ctgaaggcag ccggcgcaag
     7621 tggtaagctg gattattttt gtccgcttta cttcaggcga gccttcttaa gggccaggga
     7681 aaaggccgtc tcgcctttcg gcgcgtcttc ctgcttcggc gcggatggtg aatagcgggc
     7741 aatgatggcg gcgttttcgg ccgcgtccgg atcgacctcg ggaacggtca tttccggaac
     7801 cggggccagc gccttgattg acagggcgat tcgcctggcc gcgtggtcga tcttccttac
     7861 cgatgcctcg acccgctgcc cgatggaaag cacgtcacca gggtgtctga cgcgtcttcc
     7921 ggccgcaatc tcgctcacgt ggaccaggcc ttcgattccg ggggcgattt cgacgaaagc
     7981 gccgaagttc atcagcctgg tcaccttgcc cgcgaccgtc attccttccc tggcgacttc
     8041 atcaatcgag ttccaggggt cgtcctgcct gctgcgcagg cttgcggaaa tcctcggctt
     8101 tccatccttc tgctcgatcc ttgaaatctc gacgttcagt tcccgcccag gttccattcc
     8161 gcccaggatt tcgtcccgac cgtgccagcc aagttccgaa accggcacca gggcgtgcac
     8221 tccgcccccg atatcgacga acaccccgaa cttctcgacc ttcacgaccg tcgccgccac
     8281 ggtctggccc acggcaaggc tcgaaaggat acgtccgcgt tcctcggcca tctcctgtcg
     8341 aagaaggctg gcccgcgaca gaacggcctc atgctccctg gcttcgatga ccttgaaacg
     8401 gaacactcgc ccgatgtact gctccggagc gagggctggc ccgatttcca tctggctgat
     8461 cggcacgaag cctctaaccg gcccgatctc cacggtgaac ccgcccttgt tgtgtcccgt
     8521 gacctttgcc tcgattggaa ggtcggcctc ttttgccatc ctgattgccg acatgtcggc
     8581 gcctcgcccg ccgatgcttc ttgaaaggac ggtggccccg ccggcaaact tcactacatt
     8641 tgcctcgatt acatcgccca ccttcatcgc ggccagttct tccggcgtga agtcctccat
     8701 ggcaatcgag gcttcgctgc gctgcccgat atcgaccagc agcgttccgt cgccaaccga
     8761 gacgaccgtt cccctgacct tgtcgccaac ctggaaatag ttgaaaccgt cccccgaagg
     8821 gaacgattcg ttcagcatcg cctcgaaatc ctcggcggca aagacttcct cttcagacat
     8881 cgtggaaacc ccgcaatcca gaaaaataca tcggcccgaa ccgggccggc cgtgaccccc
     8941 ggtcgtccgg cgggtggatg atacgccgga tccggcaccg gcgaaagatg gattgcacct
     9001 tgtttgacgg catgttccaa gtgaatgctt gatgaacgtc cgataccggg taaaatatgc
     9061 cggtcaggtc aacaggcctt tattctgcct gcggaggttg ttcaatgaag ttgatgattt
     9121 cgatgcagat gttcgcggtt gtgatgtttg tggcggtttc cggttgccag gccgatgatc
     9181 gtggccaggg ctccggcggt gctgcggttg ccgggaaggc cgccaaggct gccccggccc
     9241 cggttccggt cgcgccggtc aaggtctcga tccagaccag cgtttccacc ggcggtggct
     9301 tgatcgaaat ggaactctac ccggacaagg ctccgaagac ggtcgagaat ttcgttaagc
     9361 tcgcccgcca ggggttctat gacggcgtcc tgtttcacag ggttgttccc ggtttcgtca
     9421 ttcagggcgg ggatccgttg acgaaggatc cggccatgaa gtcgcgctgg ggttctggcg
     9481 ggccggacta ccggttcgag gacgaaccgg tgaagggtga ctatgtgcgc ggcgccgtgg
     9541 ccatggccaa cgctggtccg gacacgaacg gcagccagtt tttcatctgc gtcaacgacc
     9601 ttaccggccg tcttcctaag cagtacaact ggttcggcaa ggtcacgcgc ggaatggatg
     9661 tcgtcgatgc catcgtagcg atgccccgtg atgaaaggga ttgtccgaac gaaaacgtcg
     9721 tgatgcagaa ggtcaccgtc accgattaat ttttcatcgg ctgcggcagc tcacttcggc
     9781 gcaggccccc cagaatccaa gtccgattga tgccgcccgt cgggcggcaa tgcggatttc
     9841 ttcggcccgg ccatcgtcct cctggaggat ccaggcgcag gcgtgaccac gtgaagccag
     9901 gtattccgag gcgcagcgtc cttcaacgat cagatgcgca agcagtcgtc catagtcgtc
     9961 ggtgcatctc gatgtgaatt ccagttcgac gtatttgtcc atcagaagcg catcggccgc
    10021 cgcgcgggag cgggagctcc agcagcagcc ggtctcaggc gcgtcgattc caagcagcct
    10081 gacggtccac gagtcgtcga tgacgacggt gtccccatcc agcacggcag tcacacggcc
    10141 gccccagatc ccgcagtcgt gcgtcccgga cgcgcaaccg gcgaacaatg tcgcgacaat
    10201 gcatgcggca cgagcgacat ggatgcgaca ggcttcgctt atcatcgctt tttcttcctg
    10261 gtcgtgcagg atgccgccag gcgtcccggt gttccaccat cgcagccggt cacgacccag
    10321 gccgcggcgt cgtcgtcacg ggatgcgatg gtcgcgacat cctcggtggc cgcgagatgg
    10381 acggattttg cctcaggaac tttgggatgg atggcggttg caatcatgtt gcccgacgta
    10441 tccgagaatt cgagcatcgc cgagccgccc gtggcgttgt agaaggtatc ggttccctcg
    10501 aggctggccc catcgggacc gcccaggatg attacctcgc ccaacctgac cgcgaggcat
    10561 gtttccgaga cgatcgagaa tgcccgcgac gcatggtcac gaccagggcc gttgaaatga
    10621 acgatggaga tgccgttcag gtcgaagtcc gcaagggcga tgatctccag ccattcgtcc
    10681 ttcagcgtgt cgcttccctt cgggttgaag aagacctcgg tgaccgccag gtcgtcaagc
    10741 gtcggcctga cgggccggcg gtaggttccg gtcgccggat tcctgcactc ggccccggcc
    10801 gggacggggc agcccaggtt tgtttccccg ggtgtcccgg agttcccctc ggtgtccggg
    10861 gctgcggtcg atgcacacca cgcggcgggc gttccggcct gcggcacagg ttccatctgc
    10921 agggagaagc cttcgggcgg gggaggcact ccggtacccc aagaaaagga tgcgacctcg
    10981 gtgtcgttgc acaggatggt gaccgttccg ctttcattcg gcagagtcag cgagtcaagg
    11041 gtagccgccg cggggttgcc gccaggcggc gcctcgctga agaagactcg cccgccggca
    11101 gggatggatg gtttcgtcca cccggtgacc ccggatatcc gcggccttga ttcactgccg
    11161 gacaccacga gcagcccgtc cagttcgacc ggacccgcgg agacgttggc gacttcgatg
    11221 aattcaaggc cggcatcctc gccgatcggg ttgatcagca gttccgttat caccagttca
    11281 ccaccggtgc atgtgcatcc ggtgaccacg tcgcgaaccg gcgtcgcggt ctggccgcac
    11341 cccggatgaa gaaccgtccc ggcgacggca attgcgatgc agacaaatcg atcgattcgg
    11401 cgtttcatca tcgaaccccc ttgtcagggc gggcctggcc ggtcctgccc gtgaaaagca
    11461 gatcgaacga gatggccgcg gcaatctcgt tccttccggg aaatccggat gccgatcccc
    11521 acgacggtct ctgatcgacg tgacgggtat atcggccgat ggcccttatc cgtaacagcc
    11581 cagtgctgaa ccttgcgtcc tgtgcaatcg tcaggccggt ttcgaccctg gagtcgcccc
    11641 ggcatatccc cggcacccgg ttttcgatct cccgatccag gcaggactgt ccagtagtca
    11701 ccgaggtatc ggcagccatc gactcgtcgc tgaatcttgc gaaggcggaa aggagtggcc
    11761 caggcgacag gttcgcgtcg atcttcaggg tcgccgaaag atccatgtcg aagcggtcgc
    11821 gcaaggttgc tgcatcccgg aaggcctggc caacccttat cgccagcgcc attcgccgga
    11881 tccgcttcgt ctgaaaccag accgaccagt caaccctgca gccgcccgaa aggttgccgg
    11941 tagtcgccga atagtattga tcccaggaaa ggccgcgccc cgaaacggcc agatcccggt
    12001 cgcggaagga aatctcgatg cccagccttt catgacgcga tgcgaatacc tgaagagcgg
    12061 tggcggcctc cagggccacc tgcggtcgtg attcggccag atcagaatgg ccgttccgtc
    12121 cgacgtcgat tctgaatctg ttgattgtcc tggcggaact tcgccaggtc acgtcgagtc
    12181 ttccggcaac ttcatcacgc gcgcggtttc ccctgaattc cctggagtcg gccgtggagc
    12241 cggtccatgg gttgtcccag ttgcgaccgt agtatcttat ggagggctcg agatccaggc
    12301 ctgataccgg gctgaaggct gtttcaagca tgcctgccgt ggctccgttg tcggaaaccg
    12361 cgaattcggc cgcaaggtcg ccattcagcg gcagccgcgc atgtcccgcg actccgaccg
    12421 cgccgaaccg t
//
LOCUS       DAOPPA010000026        12265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_5985_length_12265_cov_3.741523, whole genome shotgun sequence.
ACCESSION   DAOPPA010000026 DAOPPA010000000
VERSION     DAOPPA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5985_length_12265_cov_3.741523"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1648)
                     /locus_tag="PKH54_01565"
     CDS             complement(176..1648)
                     /locus_tag="PKH54_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC98603.1"
                     /translation="MSRFPALAITLLFIGSATASESQLAAETVGAMESIGVSGPDDAR
                     AATPDEAQRILSIEDVLDLALQNNPRIIRARLGTEESQAEYDAATAEMHPRIEVTTSL
                     APVPGHRIGDPATPFAGTPDRDFGDLGEWGIWARLNIKLYQPIYTSGKITWLRRAHQL
                     GVQVGKAREDIAKADVRLEVMQAYFGLMLTRKLASVLAEGRGYFDKARKHLQKMEDED
                     DPSFDPVDTMKVSVYEAQIIERELQAKRMMKLAEGQLRRLIDEDPLSETTFSLPEDLE
                     PVEPVGSPSLNELVQTGLDNRVELAALRTGIKAREAEVEARFRNFFPDFLLYGEFNVS
                     FSNVADNMPFPYYDPYNDLYLGAGLVMRFDLNIGKKLGQLKVARAKKSGLQAELDAAE
                     NGIALEISKLFMEMDDARTMMETRRNALKAARGWVIAKTDLFENDLCGLQDIIDGLTQ
                     FFLAQVSFQQSIYDFNMAVAALERATGVELVPVPGSDVEQ"
     gene            complement(1677..3179)
                     /locus_tag="PKH54_01570"
     CDS             complement(1677..3179)
                     /locus_tag="PKH54_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98604.1"
                     /translation="MGELKSSYDVVIVGTDLPALIFGALAAKNGYRVLLLGHGGRNNV
                     YEEDGFRFVRRPNLMYGFTESQSISEIFRELALLPEMRNRPRQIDPICSVVMPEKRLQ
                     ISHVRDALTHELVREFPGQAGQIENLLHNFFENDSQIDPILRGLCPVPPNGISGYFKW
                     RGIRKNLLSLAPGDDALEPVGATDARTFLSAPTAFLTGCAEPWAFPLAFSRTTNHLLR
                     GLYHVEWGLDSLKALFLNRIEGNSGTVRAADFVDVVQARRSRIHEIDVQGRNESIGAS
                     LLVAGTDLENIIERLPEKMAGRRWRQRLQETAATHAMVTLNIGMARKGLPEGMARNVF
                     LVQTPEASLDGDNLLMLQVDPAMEPADSTDPDRAVLSVSGMMPATDPESVENVERFCS
                     QMMSRVKAFMPFLDRHVFVTSIPSITLNPRTGKDMIDRMSLPMIYGKPIPGTLGLVSR
                     PIRTPVANLLNLGDSAAGLMGFEGAFTAAQVAFGMLKKQLPKKDVIQHSR"
     gene            complement(3195..3824)
                     /gene="purN"
                     /locus_tag="PKH54_01575"
     CDS             complement(3195..3824)
                     /gene="purN"
                     /locus_tag="PKH54_01575"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOC98605.1"
                     /translation="MTNVMAPGRVPIAILISGRGTNMAALVRAAASGTLNADIRLVLS
                     NKADAAGLEFANAAGIRTLVLSHRDFATREDYDRALVAHLREAGVQYVALAGFMRILT
                     HEFLGPFAGRVINIHPAILPSFPGTHAQKQALDYGVKVSGCTVHFVDEGTDTGIIIAQ
                     QTVPVLDGDDEDSLSARILAQENELYPQALNDVLCGRISVVGRRTIRKS"
     gene            complement(3821..4864)
                     /gene="purM"
                     /locus_tag="PKH54_01580"
     CDS             complement(3821..4864)
                     /gene="purM"
                     /locus_tag="PKH54_01580"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOC98606.1"
                     /translation="MTMQKLTYKQAGVDIDAGDEFASAIGRMVKSTITPEVVSGVGGF
                     AAAVAPNMLGMKRPLLISGTDGVGTKLKLAFMTGIHNTIGIDLVAMCVNDVLTTGARP
                     LFFLDYFASGHLEQKVSLQVIEGIVEGCRQAGCSLVGGETAELPGFYAAGEYDLAGFS
                     VGIVDEPDLVDGRTCKPGDVLIGLPSSGVHSNGYSLVRHIIDTRKLDIHHVYEGFTRT
                     LGEVLLTPTYIYADAVNALMAGPKPKAMAHITGGGIVGNLPRVFPQGVGAEVWMSEVP
                     ESPVFQFLQGKGTGYSVEDDEMMRVFNMGVGLIIVMAPEKADAAIEALRSVGRQPFVF
                     GRLTAGSGEVVVK"
     gene            5021..6415
                     /locus_tag="PKH54_01585"
     CDS             5021..6415
                     /locus_tag="PKH54_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC98607.1"
                     /translation="MAGLEDAGRKAGELNQHGCSMTGFAFQIMDLTGPDFAVVIFGER
                     DCVSAFPRISSEFASQPAWNVYSVALRESDVVAGRASERLRECLVAVAASMRPPVVEQ
                     PVVQGAAVVVQSIARHPSPVIVVLSTCMSEMTGAVAEQVCRQVQEGSGVRIIPVATSG
                     LRLRTQAEIVDHVAAVLLDAFGDFGTVDPAGVNLIGYQTDRFSQAAEAGTMFPDEASL
                     LISAFGGRLNSAVAAGASVSDWRNLPRGGLNVLVDRSLVGGLARLLEGQGRRFVQLAQ
                     PKGVAASDSFYAAIAREIGSDAWPVLENFQPRLEAIQAVARARARFSGMRLAYGIGTI
                     HNFRPDVLAREGLDNLPLFLELGFDVEIVIQERDYPEVHRRIRRNLELLGVDAPYRLY
                     YEPAVLEPALREGRYDCAYVADFMRDQVNRAGIPMLRLGEMNSGYRLAEKYIGRIEET
                     LGAVFDRRFGRYFH"
     gene            6428..7795
                     /locus_tag="PKH54_01590"
     CDS             6428..7795
                     /locus_tag="PKH54_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC98608.1"
                     /translation="MNKSDQAAAVPPDIGEFELRDMDAGDLLGIFMGVHSVPGVIMLL
                     HTTVGCKFKTQLQVSDHDWGRESHNQRLWTGVDDARIIKGSGERLLEFATTWYQRRKP
                     ELFVVVTNASVELSAFDVEASVRELAMRLPIPVIYLKAPGHDGSIFSGFERFTAAMIP
                     FLDSPDLSEGNRNSVALGGYFLDRFEMEHAANVNEIKRLLAGLGLAFRGTLFDGSPFE
                     KVRRAGAAVNMIWLPYAGGVAQAACAGDGRRHCVTDLPVGLTGTTAFIRKVGESFGLD
                     HAMVEGVCDRELARAVPIIRRAVSSLSGTRVGVFLDTNLAAAVTSFLTEIGCEVPVVF
                     LTDGASASRDRFMAACARLGAPFADPDPGASGLAARGPRVVAGASRNRQYLEFMRECE
                     REFIPVVVGSSLQKAAFRRGDIRVIEMGFPCAEKHWLYPMPWMGYNGAVAIVQRLLDA
                     SMRSW"
     gene            8384..9355
                     /locus_tag="PKH54_01595"
     CDS             8384..9355
                     /locus_tag="PKH54_01595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98609.1"
                     /translation="MVTKMVGFGGYGRVYQAEQLSVGKRAVAIKVLHAMHRDRPATVA
                     AFKREVSYLAMLRSPCFPRVIRTGLTPDGLPYFAMEFISGKGLDGILKSNGPLDINRA
                     VYIMDRVCEGITEMHRKDIVHRDIKPGNVIVEEGAAKAWSIWMLDLGCAKPAYEPDVP
                     QNKEDTFSFGSPPYMAPETVIKGVTNEQTDIYSLGCMAYEILTGIRAIHLKDTSADSY
                     FAYLRNVEKPVPTYRVGTIVPELPEVVEQVIHRALVRDLTARYSSVMDFRNAFLSAAG
                     PYLDRQASMSVDLTTSSNVSGFVRKDPDEIAKKSGLGGRLLLRFKKP"
     gene            9463..9750
                     /gene="groES"
                     /locus_tag="PKH54_01600"
     CDS             9463..9750
                     /gene="groES"
                     /locus_tag="PKH54_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOC98610.1"
                     /translation="MKVRPMQDRILVKRIAREEKTAGGIFIPDTAKEKPQEAIVIAVG
                     PGKVLESGELRQPGVKEGEKVLFGKYAGSEVKIDGEEHIILREDDILAVIE"
     gene            9816..11468
                     /gene="groL"
                     /locus_tag="PKH54_01605"
     CDS             9816..11468
                     /gene="groL"
                     /locus_tag="PKH54_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470961.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOC98611.1"
                     /translation="MSAKELVFGESARAKVLEGVNILADTVKVTLGPRGRNVVIEKSW
                     GSPTVTKDGVTVAKEIELADKFVNIGAQMVREVASKVSDEAGDGTTTATVLAQAIYRE
                     GAKMVAAGHGPMEIKRGIDKAVAAIVADLKTLSKPTKDFVEIAQVGTISANNDTEIGK
                     IIAEAMQKVGKEGVITVEENKSIETTLETVEGMQFDRGYISPYFVTDPERMEAALENA
                     FLLIYDKKVSSMKDLLPILEQTAKMGRPLAIIAEDIEGEALATLVVNKLRGTIQVVAV
                     KAPAFGDRRKAMLEDIAILTGGRVVSEDLGMKLENVSLNDLGNAAKVIVDKDNTTIVE
                     GAGAPEAINGRVAQIRAQIEETTSDYDREKLQERLAKLAGGVALVKVGAATEAEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALLLCQKSLDALKDLPLEQKIGVDIIRRAIEEP
                     IRMIAENAGVEGSVVVEKVRTANDPTFGYNALDDKYGNLLEAGVIDPAKVTRVALEKA
                     ASVASLLLTTECVVVEKPKKEPPMPAGGGDMGGMGGMGGMGF"
     gene            11666..12103
                     /locus_tag="PKH54_01610"
     CDS             11666..12103
                     /locus_tag="PKH54_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC98612.1"
                     /translation="MPVLFMPIFCAALVVAVSGCGATRKAVRNADLISEVDEADEYVL
                     KGMVYDSDLPVAMLFLASPCITCRPMEDVIHAVGPEYDGWVRFLKVNANNSAHAVKAL
                     KIGAIPTLVIFDGRKEIERRVGAVDKKELGAILNRVLDIDEEK"
BASE COUNT         2454 a         3490 c         3704 g         2617 t
ORIGIN      
        1 tgtacttctg gccgacgatg tcaaccggtc cgggcgcggc cacaagggcc ggggaaatcg
       61 aaagtaccag gaagaaaaca agtaccgcgg ggaatctgtt caatccttca tctccagcaa
      121 cgggtccagt ttgggtgtca ggcttgctga cagcagcatt ctatccgtcc gtggctcatt
      181 gttccacatc ggatcccgga actgggacca gttcgacgcc ggtggcccgt tccagtgcgg
      241 ccacagccat gttgaagtcg tatatcgact gctggaatga aacctgggcc aggaagaact
      301 gggtcagacc gtcgatgata tcctgaagtc cgcacagatc gttttcaaaa aggtcggtct
      361 tggcaatcac ccagccacga gcagccttca gggcattgcg ccgggtttcc atcatcgtcc
      421 ttgcgtcgtc catttccatg aacagcttac tgatctccag ggcaattccg ttctcggccg
      481 catcgagctc cgcctgaagt cccgacttct tcgcccttgc caccttcaac tgtcccagtt
      541 tcttgccgat gttaaggtca aaccgcatta ccagtccggc gcccaggtaa aggtcgttgt
      601 acgggtcgta gtaagggaac ggcatgttat ccgcgacgtt tgaaaaggat acgttgaact
      661 cgccgtaaag aaggaagtcg gggaagaaat tccggaacct tgcctcgacc tcggcctccc
      721 tggccttgat tccggttcgc agggccgcca gttcgacgcg attgtccagt cccgtctgaa
      781 ccagttcgtt cagtgaaggg gaaccgaccg gctccacagg ttccaggtct tccggaagac
      841 tgaaggtcgt ttccgaaagg gggtcttcgt caatcagcct gcgaagttgt ccttcggcaa
      901 gcttcatcat ccttttggcc tgaagctcgc gttcaattat ttgggcctca tagacgctga
      961 ccttcatcgt gtcaacagga tcaaaggacg ggtcgtcttc gtcttccatc ttctgcaggt
     1021 gcttgcgcgc cttgtcgaag tatccgcggc cttcggcaag caccgatgcc agcttccttg
     1081 tcagcatcag gccaaaatat gcctgcatga cttcaaggcg tacatcggcc tttgcgatgt
     1141 cctcgcgagc cttgccgacc tgcacgccaa gctggtgtgc ccggcgcagc catgtgatct
     1201 tgccgcttgt gtatatcggc tggtacagct tgatgttgag ccttgcccag atcccccatt
     1261 cgccaaggtc gccgaagtcg cggtcaggag tgccggcgaa aggagttgca ggatcgccga
     1321 tccggtgacc cggaaccggg gcaagcgatg tggtcacctc gattcgtgga tgcatctcgg
     1381 ccgtggctgc atcatattcg gcctgacttt cctcggtgcc caggcgggcc ctgataatcc
     1441 tggggttgtt ctgcagggca agatccagca catcttcaat tgaaagaatt cgttgcgctt
     1501 catctggggt tgccgcccgg gcatcgtccg gcccggacac gccgatagat tccatcgcgc
     1561 cgactgtttc cgcggcaagc tgactttccg atgcagtggc ggatccaatg aacaaaaggg
     1621 tgatggccag tgcagggaac cttgacatcc cacctccgtt ccagccttgg gcgatcctat
     1681 ctggaatgct ggatgacatc cttcttcgga agctgctttt tcagcattcc gaaagcgacc
     1741 tgtgccgccg tgaaagcacc ttcgaagccc atcagcccgg ctgcgctgtc accaaggttc
     1801 agcagattgg ccaccggagt cctgatgggc cttgaaacaa gtccaagggt tcccggaatc
     1861 ggcttgccgt aaatcatagg caacgacatc ctgtctatca tgtcctttcc ggttctggga
     1921 ttcagggtta tcgacggaat cgaggtcacg aagacgtggc gatccaggaa cggcatgaac
     1981 gccttgaccc tggacatcat ctgggagcag aaacgttcga cgttttcaac gctttcggga
     2041 tccgtcgccg gcatcatgcc ggaaaccgag agcaccgccc tgtcgggatc cgttgaatcg
     2101 gcaggttcca tcgccggatc gacctgcagc atcagcaggt tgtcaccgtc aaggctggcc
     2161 tcgggcgttt gaaccaggaa tacgttcctg gccatgccct ccggcagccc ctttcgggcc
     2221 atgccgatgt tcagagtaac catggcgtgt gtcgccgcgg tttcctgcag cctttggcgc
     2281 catcgtcgcc cggccatttt ttcgggcagg cgttcgatga tgttttcaag atccgtcccg
     2341 gcaaccagca gggacgcccc gatcgattcg ttccgtccct gtacgtcgat ctcgtgaatc
     2401 ctcgaacgac gagcctgtac aacatcgacg aaatcggccg ccctgacagt gccggaattg
     2461 ccttcgatgc ggttaagaaa aagcgccttc agcgagtcaa ggccccactc gacgtggtaa
     2521 aggccgcgaa gaaggtggtt tgtcgtgcgc gagaatgcca gcggaaaggc ccacggttcg
     2581 gcgcatccag tcaggaaggc ggtcggggcc gaaaggaacg tccgggcatc ggtcgccccc
     2641 accggttcca gtgcgtcgtc acccggggcc agcgacagaa ggttcttccg gatcccgcgc
     2701 cacttgaagt atccggagat accgttcggt gggacagggc agagcccccg caggatggga
     2761 tcgatttgcg agtcgttttc aaagaaattg tgtagcaggt tctcaatctg tccggcctgt
     2821 ccggggaact cgcggacaag ttcgtgggtc agggcatccc tgacgtgcga aatctgaagc
     2881 cgtttttccg gcatcacgac actgcatatg ggatcgattt gccgtgggcg gttcctcatt
     2941 tcgggaagca gtgcaagctc ccggaatatc tcgctgatcg actgggattc ggtgaatccg
     3001 tacatcaggt tggggcgcct gacgaatcgg aaaccgtcct cttcataaac gttgttgcgg
     3061 ccgccatgac caagaagaag tacccggtat ccgttctttg cagccagtgc gccgaagata
     3121 agggccggaa ggtcagttcc gacgattacg acatcatagg aactcttcag ctcgcccatg
     3181 gcaggctcct tggttcagga tttccggatg gttctgcgtc caaccaccga aatgcgacca
     3241 cacaggacat cgttaagagc ctgcgggtag agctcgtttt cctgggccag gattctggcc
     3301 gaaagagaat cttcgtcatc gccgtcaaga acaggaacgg tttgctgggc gatgattatc
     3361 ccggtgtccg tgccttcatc cacaaaatga accgtgcagc cgctgacctt cacgccataa
     3421 tcaagcgcct gcttctgcgc gtgggttccg ggaaaggacg gaagaatcgc cgggtgaata
     3481 ttgatgaccc gcccggcaaa aggccccaga aattcgtgtg tcaggatcct catgaatccg
     3541 gcaagagcga cgtactgaac gccggcctcc cgcagatggg ccacaagtgc ccggtcgtag
     3601 tcctcacggg tcgcgaagtc ccgatgcgaa aggacgagtg tccgtatgcc tgcggcgttc
     3661 gcaaattcaa ggccggcagc gtcggccttg tttgacagca ccagccgtat gtcggcattg
     3721 agcgtgcctg atgcggcagc cctgacaagc gcggccatgt tggttccacg gccggaaatc
     3781 aggattgcaa tcgggacgcg gcccggggcc atcacatttg tcacttcacc accacttcgc
     3841 cggaaccggc ggtcagtctt ccgaaaacga acggctgtct gccgacagac ctcagagctt
     3901 caatcgccgc atcggccttt tcgggggcca tcacgatgat aagtccgacg cccatgttga
     3961 agacgcgcat catctcgtcg tcctcgacgc tgtatccggt tccctttccc tgaaggaact
     4021 ggaagacggg cgattccggg acttccgaca tccagacttc agccccaacg ccctgtggaa
     4081 atacacgggg caggttgccg acgatcccgc cgcccgttat gtgtgccatg gcctttggtt
     4141 tcgggccggc catcagcgcg tttacggcgt cggcataaat gtatgtcggg gtcagcagga
     4201 cttcacccag tgtcctggtg aagccttcat agacgtgatg aatgtccagc tttctggtgt
     4261 cgatgatatg acgaaccagg gaatatccgt tcgaatgcac acccgaactt ggaagtccga
     4321 tcaggacgtc gccgggcttg catgtgcgtc catcgacaag atccggttca tcgacgatgc
     4381 cgaccgagaa gccggccaga tcatattcac cggctgcgta gaacccggga agctcggcag
     4441 tctcaccacc cacaagcgag cacccggcct gcctgcagcc ctcgacgatt ccctcgatta
     4501 cctgcagcga aaccttctgt tcaaggtgtc cgctggcgaa gtagtccagg aagaagagtg
     4561 ggcgggcgcc ggtcgtcagg acgtcgttga cgcacatcgc gacaagatcg attccgatgg
     4621 tgttgtgaat tccggtcatg aacgccagtt tcagtttggt gccgaccccg tccgttccgg
     4681 aaatcagcag cgggcgtttc attccaagca tgttgggggc gacggcggcg gcaaatccgc
     4741 cgacaccgga aacaacttcg ggagtgatgg tggatttgac catccttccg attgcggagg
     4801 cgaattcatc cccggcatcg atatcgacac cggcctgttt gtacgtcagt ttctgcattg
     4861 tcatcgcgtg ttcaccgcag ccagtttgcg gcccgaagaa tagcctgtca acgtcggccc
     4921 gtcaatcgtg acccggtcct gcggcggtgc cgtcaagggg gccgactttt tgcacaaaag
     4981 cccttgtcga tgcgctagaa tccgcgttcg gaggcatgcg atggcaggtt tggaagacgc
     5041 gggaagaaag gccggtgaac tgaaccagca cggttgttca atgaccggct tcgcatttca
     5101 gatcatggat cttaccggtc cggactttgc ggtcgtcatt tttggcgagc gcgactgcgt
     5161 cagcgcattt ccgcggattt catcggagtt cgcttcccaa cccgcctgga atgtctattc
     5221 ggtggcgctt cgggaatccg atgtggtcgc ggggcgggct tcggagcgcc ttcgagaatg
     5281 cctggttgcc gtggccgcct cgatgcgccc ccctgtcgtg gaacaaccgg tcgtgcaggg
     5341 cgccgccgtg gtggttcaaa gcatcgcaag gcatccatcg ccagttatcg tcgtcctttc
     5401 aacgtgcatg tccgaaatga ccggcgccgt tgccgaacag gtgtgccgtc aggtccagga
     5461 aggttccggg gtcaggatta tcccggtggc gaccagcggc ctgcgacttc gaacacaggc
     5521 ggaaatcgtc gatcatgttg ccgccgtgct gctggacgcc tttggcgatt tcggtacggt
     5581 ggatccagcc ggggtgaacc ttatcgggta ccagaccgat cgtttcagcc aggcagccga
     5641 ggctggaacc atgtttccgg acgaggcgtc actgctgata tcggcctttg gcggacggct
     5701 caacagtgcc gtcgcggcgg gcgcgtcggt atcggactgg cgcaatctcc cgcgcggggg
     5761 actgaacgtt cttgttgacc gatccctggt cggtggactg gcccggttgc ttgaggggca
     5821 gggcaggcgt tttgtccagc ttgcacagcc aaaaggggtc gccgcatctg attccttcta
     5881 tgcggcgatt gcacgtgaaa tcggttcgga cgcctggccc gttcttgaaa actttcagcc
     5941 ccggctggaa gcgattcagg ccgttgcccg tgcaagggcg cgcttttccg ggatgcggct
     6001 ggcatacggc atcgggacga ttcacaactt ccgtcccgac gtgcttgcca gggaggggct
     6061 tgataacctc ccactgttcc ttgagcttgg ttttgacgtc gagatcgtca tccaggaacg
     6121 cgactatccc gaggttcacc gccggatccg caggaatctt gaactgctgg gagttgatgc
     6181 gccctatcgg ctttactacg agccggccgt gctggaacct gcgctcaggg aaggtcgtta
     6241 cgattgcgcc tatgttgccg acttcatgag ggatcaggtc aaccgcgccg gaattccgat
     6301 gcttcggctt ggtgagatga attccggata ccggctggcc gaaaagtaca tcggtcgcat
     6361 tgaggaaacg cttggtgcgg ttttcgaccg gcgttttggg cgttacttcc attgagcggg
     6421 gctttcggtg aacaagtcag accaggcggc cgccgtgccg ccggatatcg gcgaattcga
     6481 actgcgagac atggatgccg gtgatctgct gggcatcttc atgggcgttc attcagttcc
     6541 cggcgtcatc atgcttcttc acacgacagt cggatgcaag ttcaagacgc agcttcaggt
     6601 ttccgaccac gactggggac gggaatcgca caaccagcgt ctgtggaccg gtgttgatga
     6661 cgcaaggata atcaagggtt ccggcgaacg ccttctggag tttgccacaa cctggtacca
     6721 gcgcagaaag ccggaattgt tcgttgtggt cacgaatgcc tcggtcgaac tttctgcctt
     6781 cgatgtcgag gccagtgtcc gcgaactggc catgcggctt ccgattccgg tgatttacct
     6841 gaaggcaccc ggtcacgacg gatcgatttt ttccggcttt gaaaggttca cggcggcaat
     6901 gatcccattc ctggacagtc cggatttgtc cgaaggcaac cggaattcgg ttgccctggg
     6961 tgggtacttc ctggatcggt ttgaaatgga gcatgccgca aacgttaacg aaatcaagcg
     7021 tcttcttgcc gggttggggc tggcgtttcg ggggacgctt tttgatggaa gtccctttga
     7081 gaaggttcgt cgggccggcg ctgccgtaaa catgatctgg ctgccctatg ccggtggtgt
     7141 ggcccaagcc gcctgtgccg gtgatggcag gcgtcactgc gttacggatc ttcctgtcgg
     7201 gctgaccgga acgaccgcgt tcatcaggaa ggttggtgag tctttcggtc ttgaccacgc
     7261 catggtcgaa ggcgtctgcg atcgcgaact tgcaagggcg gttccaatca tccggagggc
     7321 cgtcagttcc ctgtccggca caagggtcgg tgtattcctg gacaccaatc tggcggcggc
     7381 ggtgacatca ttcctgaccg aaatcggttg tgaggttccg gtggttttcc ttacagacgg
     7441 cgcttctgcc tcccgggatc ggttcatggc tgcctgcgcg cgtctcgggg ccccatttgc
     7501 cgatcccgat ccgggtgctt ccggactggc ggccaggggg ccacgcgttg tggccggcgc
     7561 atcgagaaat cgccagtatc ttgaatttat gcgggaatgc gagcgggaat tcattccggt
     7621 ggtggttgga tcgtcgttgc agaaggcggc atttcgccgt ggcgatatcc gtgtcattga
     7681 gatgggcttc ccgtgcgcgg aaaagcactg gctgtatccg atgccgtgga tgggttacaa
     7741 cggggcagtc gccattgtcc aacgccttct tgatgcatcc atgcgttcct ggtgatttca
     7801 acatgccgga aaatcatttt cgccgattga cacgcaacgc cttataattg gccggacgtc
     7861 catgcgaagt tcctgaacga ctgcggactg accgatgaca gatgacagga aatccccgcc
     7921 ttctccgcca ggaagaccat ccggcagggt gggcgggacc gacgccgttt ttccggatgc
     7981 gcccgattcg gcgtcgcggc caaccggtcg ggtgactccg ccggccgaca acgtgcgacc
     8041 ggcaccgggt gaccgtcccc cggccccacc ttcgtcacgg ccaccgtcac cgccgccgtc
     8101 acctcagcag cccccacgtg gcggacaatc ttcgccttcg ccgtcatcgg ccgctcctcc
     8161 ccagcgtccc ccgtcgccca cggttcaggg cttccagcga tcggcccctt ccggtcagtc
     8221 gaacagttcg accagctatc gggtaacgtc caattccgcg gtttccagtt cgattaccgc
     8281 cccggatccc tctcggtcgg gcgcatccaa tgccagcggc gggtatcgtg atccattcgg
     8341 aaaggacgac aggctcgagg gcacgattct ttccgatgat ttcatggtca cgaagatggt
     8401 cggtttcggt ggatacggac gcgtttatca ggccgaacag ctttcggtgg gcaagcgtgc
     8461 cgtcgccatc aaggtgttgc atgcgatgca tcgggatcgg ccggcgaccg tcgccgcctt
     8521 caagcgcgaa gtgtcctatc ttgcgatgct tcgttcacca tgttttccgc gtgtcatccg
     8581 caccggtctg acgccggacg gcttgccgta ttttgcgatg gagttcattt ccggcaaggg
     8641 cctggacgga attctgaagt cgaacggacc gctggacatc aatcgggccg tttacatcat
     8701 ggatcgcgtg tgtgaaggca tcaccgagat gcatcgcaag gacatcgttc accgggatat
     8761 caagccggga aacgtgattg tcgaggaagg cgcggccaag gcctggagca tctggatgct
     8821 ggatcttggt tgtgccaagc cggcttatga accggacgtg ccgcagaaca aggaagacac
     8881 cttcagtttc ggcagtccgc cttacatggc gccggaaacc gtcataaagg gcgtcacgaa
     8941 cgagcagacc gacatctatt cgcttgggtg catggcttac gaaatcctga ccgggattcg
     9001 cgcgattcac ctgaaggaca caagtgccga ttcgtatttc gcctatctgc gcaatgtcga
     9061 gaagcctgtt cccacatatc gggttgggac tattgttccc gaacttcccg aggtcgtcga
     9121 acaggtcatt catcgtgcgt tggttcgtga cctgactgca agatactcat ccgtaatgga
     9181 tttccgcaac gccttccttt cagccgcagg gccgtacctg gatcgccagg cttcgatgtc
     9241 ggtcgatctg accacgtcat cgaatgtctc cggtttcgtc cgaaaggatc ctgatgaaat
     9301 cgccaaaaag tccggtcttg gcggccggct gctcttgcgt ttcaagaaac cgtgattaga
     9361 aattgattcg cccacggcaa tgggccggtt tcttactgat tccgcggtgc gaccggttgt
     9421 cgcgcgcggg tggactgcaa gatattcgtg gaggaaaagc agatgaaagt cagaccgatg
     9481 caggatcgaa ttctggtgaa gaggatcgcc agggaagaaa agacggccgg cgggatcttc
     9541 attcccgata ccgcgaagga aaagccccag gaggcaatcg ttatcgcggt cgggccggga
     9601 aaggttcttg agtccggtga actgcgccag cccggcgtga aggaaggcga aaaggttctt
     9661 ttcggcaagt acgccggaag cgaagtcaag atcgacggcg aagagcacat catcctgcgc
     9721 gaagacgaca tccttgctgt tatcgagtaa tcgtccgggc cgggaatgct ggtttcgcgc
     9781 cccgaagcgg ggcgattcgt taaggagtac aagccatgtc agcaaaagaa ctcgtttttg
     9841 gtgaaagcgc cagggccaag gttcttgaag gcgtcaacat ccttgcagat accgtgaagg
     9901 tgactcttgg gccgcgcggc cgcaacgtcg tcatcgagaa atcctggggt tcgccgaccg
     9961 tgaccaagga cggcgtcacc gttgccaagg aaattgaact ggccgacaag ttcgtcaata
    10021 tcggcgccca gatggtgcgt gaagtcgcgt cgaaggtttc cgacgaagcc ggtgacggca
    10081 ccacgacggc caccgtgctt gcccaggcca tttaccgcga aggcgccaag atggttgccg
    10141 cgggccacgg accgatggaa atcaagcgcg gcatcgacaa ggccgtggcc gccatagtgg
    10201 cggacctgaa gacgctttcc aagcccacta aggacttcgt ggagatcgcc caggtcggca
    10261 ccatttccgc caacaacgac accgaaattg gaaagatcat cgccgaagcg atgcagaagg
    10321 ttggcaagga aggcgttatc accgtcgagg aaaacaagtc tatcgagacg acgctcgaga
    10381 cggtcgaggg catgcagttc gatcgcggtt acatcagtcc gtacttcgtg acggatccgg
    10441 aaaggatgga agcagccctg gaaaacgcct tcctgttgat ttacgacaag aaggtttcca
    10501 gcatgaagga tcttcttccc atcctggaac agactgccaa gatgggccgt ccgcttgcga
    10561 ttatcgccga ggacatcgag ggcgaggcgc tggcgaccct ggtcgtcaac aagctgcgcg
    10621 gaaccatcca ggtcgtggcc gtcaaggccc ctgctttcgg cgaccgccgc aaggcaatgc
    10681 tggaagacat cgcgatcctg accggtggcc gcgtggtttc cgaagacctg ggcatgaagc
    10741 ttgaaaacgt ctccctgaat gaccttggca atgccgcgaa ggtcattgtc gataaggaca
    10801 acacgaccat cgtcgagggc gccggtgctc cggaagccat caatggtcgt gtcgcccaga
    10861 ttcgcgccca gatcgaggaa acgacgtcgg attacgaccg cgagaaactg caggagcggc
    10921 tggcgaagct ggccggcggc gtcgccctgg tgaaggtcgg cgcggcgacc gaagccgaga
    10981 tgaaggaaaa gaaggctcgc gtcgaggacg cactgcacgc gacccgcgcc gccgttgaag
    11041 aaggcgtggt tcccggtggc ggcgttgctc tgctgctgtg ccagaagtcc ctggatgcgc
    11101 tgaaggacct gccgctggaa cagaagattg gcgtcgatat catccgtcgc gcgattgagg
    11161 agccgatccg catgatcgcg gaaaacgcgg gcgtggaagg cagcgtggtc gtcgaaaagg
    11221 ttcgcaccgc caatgacccg accttcggtt acaacgccct tgacgataag tacggcaacc
    11281 ttctcgaagc cggcgtcatc gacccggcca aggtcacccg cgttgccctg gaaaaggcgg
    11341 ccagtgtcgc ttccctgctt ctgacgaccg aatgcgttgt cgtcgagaag ccgaagaagg
    11401 aaccgccgat gccggcgggc ggcggcgaca tgggcggaat gggcggaatg ggcggaatgg
    11461 gattctagtc cacaccgcag acgaacaatg tcacgccgaa agcccaggtt gatccggggc
    11521 gaaggcggat ggttcgcgat tcgttcaaat gcgaaaaagg cccttgtgac ttcggtcgca
    11581 agggcctttt ttgcatgatc ccggcaccat gtgatatctt ccaaccattc agtttcgtta
    11641 acgttcggag gtgcctgtga aaccgatgcc ggtgcttttc atgccgattt tctgcgcggc
    11701 gctggtggtg gctgtgtccg gctgcggggc aacccggaag gccgtgcgga atgcggatct
    11761 gatttccgag gtcgatgaag ccgacgaata cgtgctgaag ggcatggttt acgattcaga
    11821 tctcccggtc gccatgctgt tcttggccag tccatgcatt acgtgtcgcc cgatggagga
    11881 cgtgattcat gccgtcggtc cggaatacga tggatgggta cgcttcctga aggtcaacgc
    11941 gaacaattcc gcccatgcgg tcaaggcctt gaaaattggc gccatcccga cgctggtcat
    12001 ttttgatggc cgtaaggaaa tcgaacggag ggtgggtgcc gttgacaaaa aggaacttgg
    12061 tgcaattctg aaccgcgtcc tggatatcga tgaagaaaaa tagtcgatgt gagtcgctgc
    12121 cgttctttaa ttcgcattaa taagaccttg acagtcctga attcgatcgc attatctggc
    12181 aacgatgtcg gcgtttccga cgctggccgg gacatcatcg gacagggccc ggaagacccg
    12241 ttgcttccga agaggggcct ttgaa
//
LOCUS       DAOPPA010000027        12005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6143_length_12005_cov_3.825356, whole genome shotgun sequence.
ACCESSION   DAOPPA010000027 DAOPPA010000000
VERSION     DAOPPA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 12005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12005
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6143_length_12005_cov_3.825356"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="panB"
                     /locus_tag="PKH54_01615"
     CDS             complement(<1..703)
                     /gene="panB"
                     /locus_tag="PKH54_01615"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOC98613.1"
                     /translation="MRNLTAQSFKSKKGQGKLVVLTCYDATFASLISKTSIDAILVGD
                     SLGNVIQGRSDTLEVTLDDVIYHTRCVRRGAGQDAHIIADMPFMTFQVSTQEAMRNAG
                     MLLSVGRANAVKLEGGVRVAETISRLVQAGIPVMGHVGLTPQSFNEMGGFKIQGRDAG
                     AAAAILEDAVAVAEAGAYSIVLEGIPASLAARITETVGIPTIGIGASANCDGQVLVLY
                     DMLGMNPDFNPRFLKK"
     gene            955..1476
                     /locus_tag="PKH54_01620"
     CDS             955..1476
                     /locus_tag="PKH54_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOC98614.1"
                     /translation="MNFRNACALVAVAVVMAFSVPVMAQTVKIGVINMQKALSQTIEG
                     KAALKKLEDMQKRMEKELKAKQDEIVRLEEDLKSQVPLMNDDMKRQKLGEFQKKAADF
                     QEAYQNSAKKIGEEQQKLMAPILARFEKIVTKLGKDENFTAILHGEVVLYSSASIDLT
                     DRISKMYDSGAGK"
     gene            1525..2217
                     /locus_tag="PKH54_01625"
     CDS             1525..2217
                     /locus_tag="PKH54_01625"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC98615.1"
                     /translation="MRRFFPKPTRAVGIILFACIAIHVVFLLSIKHAPVWAMRMLEYG
                     ALTPATVLHRPWTIITMAFLHDPHSLMHIIFNMMALWSIGPWLERALGTRRFSILYAI
                     SLLAGSLVFVLKGFVSGGVSGPAIGASGAIMGVVVGFGFVFPEVELRLFGVAPLKAKN
                     IFWAAILVDTIMVIMNVPIAVSVHIGGMLGSWLYLRRPWNPRYRMWLSNRIRNKINWL
                     RIKYRLWKASRP"
     gene            2214..3857
                     /locus_tag="PKH54_01630"
     CDS             2214..3857
                     /locus_tag="PKH54_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98616.1"
                     /translation="MNETRLKFAGCLIIAAAAVAAYFPVFSNSFAWDDEYLVLKNPAI
                     HDLSSIPGFFTDSWAGGVDYELGQAQNKPYFRPMALTSMTLDWAVAGPDPVVFHATNL
                     MIHIVAAWCLFLWLLKVFSDTPRNASGTFRRIPTPVLAAFVGAIGWAVHPVSTEAVNL
                     VSYRTSLISGLTIFAGLMLLTPRSRADEPGRSGIAAVIGATLLYAVGLLSKETTLVMP
                     GLLFVMDIAAGRLRFRQKGEKGRRLLSGPTAMRIARVYVPLAIVAIVWLGLRSRFTGE
                     GVYTYFDGLTFGQSILMFGRIFYLYVRLAVAPWPLCPFYDWGILGVPKSILEPDIAAG
                     FILFALIFGGAVRFVRRNVLASAGLFFFLVALLPVSHIIPFFDAAGDRFMYVPLAGLA
                     LAAGGAAISSNSARRFQVPLKIVSFLVIAVFMLLSAARSAQWKDSETMLRVTTRDFPT
                     SISAHLGLGRLLLEKSRPTEAVVPLKHVTDIAPNLAIGHALLAVAHARNGDIAAARLT
                     LRNSPMPPSGEVSAIQLARSELIKSGNERLAMRLGLMSP"
     gene            4048..5190
                     /locus_tag="PKH54_01635"
     CDS             4048..5190
                     /locus_tag="PKH54_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98617.1"
                     /translation="MKRSNRLRILTTPLAVILPLLVLSSCGQDHHDVMVPWESFTPTT
                     VATMVARLPGADGPSGTIRIVGEATINGRTFQSYKVALDPEHPQNGIELWIEKEDENT
                     FTFQGYEEPGVMSVVANQPHTIHTDGPEGVPEIVTFNATSTTFESGESLPGTAVIEYV
                     KVSDDETIDTAFGTLSGVKHFSGTVTLQGEAAPALVAGTPLDVEIWYHPSFGLVKGDM
                     PLLGVGLDMAGEDDCGDPLTPGFNTIRKVGVVQPGGEPFRLSTYDCSGKFNADKNTHA
                     KMLLELRFADDEMARTTTPPPVFETFGTILGYFPEYLTPSSLSIFHPEENGQGYTFWY
                     ALVDQAAKNIDGDDGISYRIEVEPADYMTNAVRATARIFYKVIPVP"
     gene            complement(5275..>6517)
                     /locus_tag="PKH54_01640"
     CDS             complement(5275..>6517)
                     /locus_tag="PKH54_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98618.1"
                     /translation="HESVEVGTGCGDGLTCDDNGACTGCATADDCPGKDTECGHRTCD
                     GLVCGFVAEPAGTQIQDQDAGDCSRAVCDGSGGIGLVPDDTDLPVDGKECTVDSCMAG
                     VASNHPLDPGTPCTQGGGTKCDGSGACIQCQVASECGVDNACRQVVCNAGFCGEILTP
                     EGEVVVNPVVGDCRSAQCDGNGNIVSDAPDDQDLPVDGKVCTQDVCLDGQLSNPPLPP
                     GTPCAQNGGMQCDGDGNCVQCMVGSDCGVSTPCLEFLCLAGICGANFVQAGITVANPQ
                     VGDCRSAQCDGMGNIVPDAVYDTDLPVDGKECTQDVCLEGQLSNPPVPPGTPCTQSGG
                     NVCDGKGVCVECLKAADCDDAQLTCINSLCFPAHCVDEMENADERDLDCGGSCPPCGS
                     GRKCFDSNDCVSGVCADHVCS"
     assembly_gap    6518..6527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6528..7159)
                     /locus_tag="PKH54_01645"
     CDS             complement(<6528..7159)
                     /locus_tag="PKH54_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98619.1"
                     /translation="MTFLRNFVALIFIGLIAATMGCGTGDTVADVENEDLVSDQVEVP
                     DGQDHDAADAGADAGEGADIGPDQAVLNDVIPDVGEDAADTVEDAAEVGEDAAEVGED
                     AAEVGEDAADIIEDAAEVGEDVEDVLDDVDEVAEDADAGPCTDSSQCPATGNPCTMPS
                     CDDGECVAVPLPVDFQPLEQVPGDCQVLICDGNGGVTNVSYDEDVPDDENP"
     gene            complement(7290..7727)
                     /locus_tag="PKH54_01650"
     CDS             complement(7290..7727)
                     /locus_tag="PKH54_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protease subunit"
                     /protein_id="HOC98620.1"
                     /translation="MPVIAGAKDMATKQKPTKEPVMKGKLFVWALFLAGLFGIFMWGV
                     PTYRVWSAEMHGRAQKAEAEYSRDTIKIEAAARAEAEVTRAKGVAQANRIIADSLKGN
                     QDYLHYLWIQTLSEDNKTIVYIPTEANIPIMEATRAVTPDRVE"
     gene            8199..8819
                     /locus_tag="PKH54_01655"
     CDS             8199..8819
                     /locus_tag="PKH54_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOC98621.1"
                     /translation="MSPTQKQTRAEENAVQIIRDRGGVVRTSEAVRAGIHPRTIYSLR
                     DSGELEQLSRGVFRLSDKGSISQPDLVIVATRIPQAVVCLTSALVFHEITTQIPDKIF
                     IALPKGAEQPRLDFPPLSIHRFSGPALVEGIEIHDIDGVKVRVYCPEKTLADCFRFRN
                     RIGMDIVLEALKLYRARRRFNLSELVRYARLCRVETVMRPYLEALL"
     gene            8816..9718
                     /locus_tag="PKH54_01660"
     CDS             8816..9718
                     /locus_tag="PKH54_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOC98622.1"
                     /translation="MKQLKNVSASVRQRLLNHSRERHQPFGQVVQYYAMERFLYRLGR
                     SALVDRFILKGALMLQVWHSPQSRPTMDIDMLGRTSNDEAAVVAQIREIISTDVEPDG
                     IQFDPDSIRTEDITREADYDGIRIRFQGKLDSVRINMQIDIGFGDPVFPGPEMAELPT
                     LLDSPTPRLFCYSRESAIAEKFHAMVIRGHLNSRMKDFFDIWLLSRSFAFDSEKLAKA
                     VRLTFDYRHTAVPENLEVFDPAFVASKTGQWTAFRKKFSHLHIPESFDVIVLDISDFL
                     RPVIAGGDASSSPKHWEPQGPWRD"
     gene            10225..11136
                     /locus_tag="PKH54_01665"
     CDS             10225..11136
                     /locus_tag="PKH54_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC98623.1"
                     /translation="MVKIKWTMMIATALTSALILTMGCGDDTHGVNDTDDVVDAADAE
                     DATGLDGMMFVPSGSFWMGCNDAAANNCNPQERPYHLVTLDSFYVDRTEVTIKDFQVC
                     VDAGVCSHHLDDQTCFYIILENGDLAQSGIWPAFRGTNQPMVCVDWEQAKQYCEWAGK
                     RLPTEAQWEKAARGTDGRVYPWGNDNPDCNNAVTYVDDGSECAPEVTADVCNKSPAGD
                     SPYGLCDMAGNVREWVADWYDSNYYDTSPTTNPKGPDTGLYRTIRGSSFYPGNDLTNS
                     GRLGAEASKAFVDRGFRCVWSAESSNQ"
     gene            complement(11535..>12005)
                     /locus_tag="PKH54_01670"
     CDS             complement(11535..>12005)
                     /locus_tag="PKH54_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HOC98624.1"
                     /translation="DVQIFTFYCERQQVWVAAEVMSFGTLSFMYKGLNGSDQAVLARP
                     FGVNKVVVQSWLHTLAFVRNICAHHSRLWNRELSVAPKFPYEAAWKPPAIPDPKKVYG
                     VLCILRHLSIDEPCGDKWRRQIVGLLGRWDAEPRWRKAMGIPVDWKNSVFWATK"
BASE COUNT         2523 a         3633 c         3263 g         2576 t
ORIGIN      
        1 atttcttcag gaacctcgga ttgaaatccg gattcatccc aagcatgtca taaagaacca
       61 gaacctgacc gtcgcaattc gccgacgcgc cgatcccgat tgtcggaatg ccaaccgttt
      121 cggtaatcct tgcggccagc gacgcgggaa tgccctccag cacaatcgaa tatgccccgg
      181 cctcggcgac agcgaccgcg tcttccagga tcgccgcggc ggccccggca tcacgcccct
      241 ggatcttgaa gccgcccatc tcattgaacg actggggcgt taacccgaca tgccccatca
      301 ccgggattcc ggcctgaacc agccttgaaa tcgtctcggc gacgcgcacg ccgccttcca
      361 gcttcacggc gttcgccctt ccgaccgaaa gcagcatccc ggcgttgcgc atcgcctcct
      421 gggtactgac ctggaacgtc atgaaaggca tgtcggcaat gatgtgggcg tcctggccgg
      481 cgccacgccg cacgcacctt gtatgataaa tgacgtcgtc cagcgtaact tccagcgtgt
      541 ccgaacgtcc ctgaattacg tttccaagcg aatcgccgac cagtatcgca tcgatggacg
      601 tcttggaaat cagactggca aatgtcgcgt catagcaagt caggacgacc agttttccct
      661 ggcccttctt ggacttgaaa gattgagctg tcaaatttcg catcggttgc cccagtgttt
      721 ctcgaccccg cggattgaac cgtgacggtg gccggaatgt gggccaaggg tatcgcacca
      781 ttcccccggg ggcaatagcc cggaataaat caccggcgcc gccgttaggt tcatcaaggt
      841 tgacttgaac gaccgattgt ccgaatattg cgcggacgtt ttttcccgtc ccggccggat
      901 gacctgtcgc gacggtcggt tctcggaatc gtttccaaac tggaggaatc ccggatgaat
      961 ttccgtaatg cctgtgctct tgtcgctgtt gccgttgtga tggctttttc ggtgcccgtg
     1021 atggcccaga ccgtcaagat tggcgtgatc aacatgcaga aggccctgtc ccagacgatt
     1081 gaaggcaagg ccgcgctgaa gaagcttgaa gacatgcaga agcgcatgga aaaggaactg
     1141 aaggcgaagc aggacgaaat cgtcaggctg gaagaagacc tgaagagcca ggttcccctg
     1201 atgaacgacg acatgaagcg ccagaagctg ggtgaattcc agaagaaggc cgccgacttc
     1261 caggaagcct accagaacag tgccaaaaag atcggcgaag aacagcagaa gctgatggcc
     1321 ccgatcctgg cccgttttga aaagatcgtc acgaagcttg gcaaggacga aaacttcacc
     1381 gcgattcttc acggcgaagt cgtcctttat tcatcggcgt cgatcgacct gactgaccgc
     1441 atttccaaga tgtacgattc gggcgccggc aagtaagcgc cccacctgtc caggagcatt
     1501 aacgtgcgcc gcggatttcc aggattgcgg cgattctttc ccaagcccac gcgggccgtc
     1561 gggatcatcc ttttcgcctg catcgccatt cacgtcgtat tccttctgtc gatcaagcac
     1621 gcgcccgtct gggcaatgag aatgctcgag tacggcgcgc tgacgccggc gaccgtcctt
     1681 catcgtccgt ggaccatcat cacgatggct ttcctgcacg atccccacag cctgatgcac
     1741 atcatcttca acatgatggc cctgtggagc atcggtccgt ggctcgagcg cgcgctgggc
     1801 acccgccgct tttccatttt gtacgccatc tcgctgctgg ccgggtcgct cgtcttcgtt
     1861 ctgaagggct ttgtttccgg gggagtgtca ggcccggcaa tcggggcttc cggggccatc
     1921 atgggtgtgg tggtcggctt cgggttcgtt ttcccggaag tcgagttgcg tcttttcggc
     1981 gtggcgccgc tgaaggcgaa gaacatcttc tgggcggcca tccttgttga cacaatcatg
     2041 gtcatcatga atgtgccaat cgcggtgtcc gtgcatatcg gcggcatgct cgggtcatgg
     2101 ctttacctga ggcgaccctg gaatccgcgc taccggatgt ggctgtcgaa tcgtatccgc
     2161 aataaaatca actggcttcg aatcaaatac cgcctgtgga aagcgagcag accgtgaatg
     2221 aaaccagact gaagttcgcc ggatgcctga tcatcgcggc cgccgccgtg gccgcatatt
     2281 ttccggtctt ttcgaactcg ttcgcctggg atgacgaata cctggttctg aaaaacccgg
     2341 ccatccatga cctttcgagc atcccgggct tcttcacgga ttcctgggcc ggcggcgtcg
     2401 attacgaact cggccaggcc cagaacaagc cgtatttcag accgatggcg ctgacttcga
     2461 tgaccctgga ctgggccgtt gccggaccgg atccggtcgt ctttcatgcg acgaacctga
     2521 tgatccacat tgtggcggcc tggtgcctgt tcctgtggct gctgaaggta ttttcggata
     2581 cgccacgtaa cgccagcggg acgttccgcc gcattccgac gcccgtgctg gctgccttcg
     2641 tcggcgccat cggctgggcg gttcatcctg tttcgaccga ggccgtcaac ctggtttcgt
     2701 atcgcaccag cctgatttca ggcctgacca tcttcgcggg gctgatgctt cttacgccaa
     2761 gatcccgggc cgacgaaccg gggcgaagcg gcattgcggc cgtcattggc gcaacgctcc
     2821 tgtacgccgt cggactgctg tcaaaagaaa ccacgcttgt gatgccggga cttcttttcg
     2881 tcatggatat cgcggccgga cgtctgcgct tccgtcaaaa gggggaaaag ggtcgccgcc
     2941 ttttgtcggg cccgaccgcg atgcgcatcg ccagggtcta tgtaccgctt gcgattgtgg
     3001 cgattgtctg gcttggtctt cgatcccggt tcacgggcga aggcgtttac acgtacttcg
     3061 acggcctgac gttcggccag tcgatcctga tgttcggccg catcttttac ctttacgtca
     3121 ggctggccgt ggcgccctgg ccgctttgcc ccttctatga ctggggcatc ctcggcgtcc
     3181 cgaagtcgat tctggaaccc gacatcgccg caggcttcat actgttcgcg ctgatcttcg
     3241 gcggggcggt caggttcgtc cgacgaaacg tgctcgcttc ggccggattg ttcttcttcc
     3301 tggtcgcgct gcttcccgtg tcccacatca tcccgttctt tgacgccgcg ggcgaccgat
     3361 tcatgtacgt tcccctggcc ggtctggccc tggccgcggg tggggccgcg ataagttcga
     3421 attccgcccg caggttccag gttccgttga agatcgtgtc ttttctggtt attgccgtct
     3481 tcatgctgct ttcagccgcc cgttccgccc agtggaagga cagcgaaaca atgctgcgcg
     3541 taaccacgcg ggacttcccg acatcgattt cagcacacct cggacttggg cgcctgctgc
     3601 tggaaaagag ccgtccaacc gaagccgtgg ttccgttgaa gcacgtcacc gacatcgcgc
     3661 cgaacctggc gatcggtcac gcacttctgg ccgtggcgca tgcccgcaac ggcgacatcg
     3721 ccgccgcccg actgaccctg cgaaactcgc cgatgcctcc ttccggagaa gtctcggcca
     3781 tccaacttgc ccgcagtgaa ctgatcaaat caggcaatga aaggctggcc atgcgccttg
     3841 gcctgatgag tccttgacag accggaagaa gagtctttcc aattacactt cgatctccgc
     3901 acgcttccga cctttctcca ttgcaccgga tgcatcctgc caacgagtcc tgcccgcata
     3961 taagcaattt gcgacacagg cgccggttgt ttgacaccag gttcaatcgc gtggcagact
     4021 tcccccgatt gcaaccgagg attttcgatg aaacgttcga atcgcctgcg catcctgacg
     4081 acgccgcttg ccgtgattct tccgctgctg gtgctttcct catgcggtca agatcaccat
     4141 gacgtcatgg ttccgtggga atccttcaca cctacgaccg ttgccacgat ggtcgcgcgc
     4201 ctgcccggcg ccgacggccc gtccggcacc atcagaatcg tcggggaagc cacaatcaac
     4261 gggcgcacat tccaatccta caaagtcgcc ctggatcccg aacatcccca gaacggcatc
     4321 gaactgtgga tcgagaagga agacgaaaat actttcacct tccagggtta cgaagaaccg
     4381 ggagtgatgt cggtcgtggc gaatcagccc cacacgattc acacggacgg ccccgaaggc
     4441 gtcccggaga ttgtaacctt caacgcgaca tccaccactt tcgagtcggg cgaatcactt
     4501 cctggaaccg cggtcatcga gtacgtgaaa gtgtctgacg acgagacgat cgataccgcc
     4561 ttcggcactc tgtccggggt caagcacttc tcgggaacgg tcacgctgca gggagaagcc
     4621 gccccggccc tggtcgcagg aacgcccctg gacgtcgaaa tctggtacca cccatcgttc
     4681 ggcctggtca agggggacat gccgcttctg ggcgtgggtc ttgacatggc gggcgaggat
     4741 gattgcggcg acccgttgac gccaggtttc aacaccatcc gcaaggttgg cgtggttcag
     4801 cccggtggcg agccattccg gctttcgacg tacgactgct ccggcaaatt caacgccgac
     4861 aagaacacac acgccaagat gctgctggaa ctgcgattcg ccgacgatga gatggcccgg
     4921 acaaccacac caccgcccgt tttcgaaact ttcgggacga tcctgggcta tttcccggag
     4981 tatctgacgc cttcctcgct cagcatcttc caccccgaag aaaacggcca gggttacaca
     5041 ttctggtatg cactggttga ccaggccgcg aaaaacatcg atggggacga cggcatctct
     5101 taccgaatcg aagtcgaacc ggccgattac atgacgaatg ccgtgcgcgc gaccgcccgc
     5161 atcttctaca aggtgattcc cgtgccgtga cggccgatcg ggccgatcca ctgaagaatt
     5221 gacaacagaa cgaagccacg ggaacgcctg tgtgcgccag gctcgggaat ccggtcagga
     5281 acatacatgg tcagcacata cgccgcttac gcagtcattc gaatcgaagc acttgcggcc
     5341 tgaaccgcat ggcggacacg acccgccgca atccaggtcg cgttcatcag cgttctccat
     5401 ttcgtccacg cagtgagccg ggaaacacag gctgtttatg caggtcaact gagcgtcatc
     5461 gcagtcggcc gccttcaggc actcgacgca tacgcccttc ccgtcgcaca cgttgccgcc
     5521 gctctgcgta cacggcgtac ccggcggtac cggcgggttg ctcaactggc cttccaggca
     5581 gacgtcctga gtgcattcct tcccgtccac cggcaggtcg gtgtcgtaaa ccgcgtccgg
     5641 cactatgttt cccattccgt cacactgggc cgacctgcaa tccccgacct ggggattggc
     5701 aaccgtgatt ccagcctgca cgaagttcgc accgcatatg ccggcaaggc acaggaattc
     5761 aaggcaaggt gtactgactc cgcagtccga tccgaccatg cactgaacac aattgccatc
     5821 gccgtcgcat tgcatgccgc cgttctgagc gcaaggcgtt cccggcggta acggcggatt
     5881 gctcaactgg ccgtccaggc agacatcctg ggtgcatacc ttgccgtcca caggcaagtc
     5941 ctggtcgtcc ggggcgtcag acacgatgtt cccatttccg tcacactggg ccgacctgca
     6001 atccccgacc accgggttga ccacgacttc gccctcgggc gtaaggatct cgccacagaa
     6061 gcccgcgttg cacacgacct gccggcaggc gttgtccacc ccgcattcgg atgccacctg
     6121 acactggatg cacgctccac tgccgtcaca tttcgtgccg ccaccctgcg tacacggagt
     6181 acccggatcc aacggatggt ttgatgcaac gccggccatg caggagtcca cagtacactc
     6241 cttcccgtcc actggaaggt cggtatcgtc aggaacgagc ccaatcccgc cggagccgtc
     6301 gcacacggcc cgactgcaat cgccggcgtc ctgatcctgt atctgcgttc cggccggctc
     6361 ggccacaaaa ccgcatacca gaccatcaca ggttcgatga ccacattccg tgtccttgcc
     6421 agggcagtcg tccgcggtag cgcatccggt gcacgccccg ttgtcgtcgc aggtcaaacc
     6481 gtcaccgcag cctgtcccga cttccaccga ctcatgannn nnnnnnngga ttttcgtcat
     6541 ccggcacgtc ttcgtcgtaa ctgacattgg tcacgccgcc attgccgtcg caaatcagca
     6601 cctgacagtc accgggaacc tgttcaaggg gctggaaatc gactggcaac ggtacggcca
     6661 cacactcgcc gtcatcacac gacggcatgg tgcatggatt tcccgtcgcg gggcactgcg
     6721 agctgtcggt gcaagggccc gcgtccgcat cctcggcgac ttcatcgaca tcatcaagga
     6781 catcctcgac gtcttcgccg acttccgctg catcttcgat gatatccgct gcatcctcgc
     6841 cgacttccgc tgcatcctcg ccgacttccg ctgcatcctc gccgacttcc gctgcatcct
     6901 cgacggtgtc cgcagcgtcc tcgccgacat ccgggatgac atcgttcaac acagcctgat
     6961 cgggcccgat atccgctccc tctcccgcat ccgcacctgc atcggccgcg tcatgatcct
     7021 ggccatccgg gacttccacc tggtccgata ccaggtcttc attctcaaca tcggcaaccg
     7081 tgtcaccggt cccgcacccc atggtcgccg cgatcaggcc gataaagata agcgccacaa
     7141 aattccttaa aaaggtcatg gtatcttccc gaatcgtgaa ttggggatag tctctgtcga
     7201 atctcgccat cagtcaatct cgacaggttt tgtcttgtcc cccgaaggtg gttgacattt
     7261 ttgcggcggg ttaatggccc gtatccccct cactccaccc tgtcgggggt gacggcgcgg
     7321 gtggcttcca tgatggggat gttggcttcg gtggggatat atacgattgt cttgttgtct
     7381 tcggacagcg tctgaatcca caggtaatgc aggtaatcct ggttgccttt caggctgtcg
     7441 gcgatgatgc ggttggcctg ggcgacgccc ttggcccggg tgacttcggc ttcggcccgc
     7501 gcggcagcct cgatcttgat cgtgtcacgg ctgtattcgg cctcagcttt ctgtgcccgg
     7561 ccgtgcattt cggccgacca cacccgatat gtcgggacgc cccacatgaa tatgccgaaa
     7621 agcccggcca gaaacaaagc ccagacaaac aattttcctt tcatcacagg ttcctttgtt
     7681 ggtttctgtt ttgttgccat atctttcgca cccgcgatga ccggcatacc gcgccccggg
     7741 ccgtttccat acacttcgcg cgatgttaat cctaacttta caaacttgaa agcggcagac
     7801 aggcaggtaa gatgcggaat aatgacctgc cccccaaaaa cgtgacggtt caaacctggg
     7861 acagtatgga aacccgacct cacgccaggt tacaacggcg cttgcgccgg taagctcttg
     7921 gcgcacgggc gcgcgtattt acgcgcttca tgctgacgca cgggcacgcg tgtttgcgcg
     7981 cttcatggac ctcttgaatg ccatcgagag atcaacggca tgatttcggg gagttgcggg
     8041 acatttcgca gcccgccacg ggaattgtta cgaaatttcg cgaagttgcg acggcttacc
     8101 atccccgcac attctctggc acttcagctt atcttgcctt gaccggaatt ctggataata
     8161 atacctattg gcagacgcag cgattggtaa tatttgccat gagccccaca caaaaacaga
     8221 ctcgagccga ggaaaacgcg gtacagatca ttcgggatcg aggcggtgtg gttcgtacgt
     8281 ctgaagctgt ccgcgccgga atccacccgc gcacaatcta ctcgcttcgg gattcggggg
     8341 aactggagca actttcaaga ggtgtcttca gactttctga taagggctcg atatcccaac
     8401 cggaccttgt cattgtggcc accaggattc cccaggccgt cgtgtgcctt acctccgccc
     8461 tggtatttca tgagatcacc acccagatac ctgacaagat tttcatcgca cttccgaaag
     8521 gcgccgagca gccacggctt gattttccgc cactgtccat tcaccgcttt tccggtccag
     8581 cccttgtgga aggtatcgag atccacgata tcgatggtgt caaagtcagg gtttattgtc
     8641 cggaaaagac cctggccgac tgttttagat tcagaaacag aattggcatg gatatcgtac
     8701 ttgaggcgct gaagctgtac cgggcacgtc gtcgcttcaa tctgtcagag cttgttcgat
     8761 acgcccgact ctgccgggtt gaaacagtca tgcgcccgta cctggaggct ctcttgtgaa
     8821 acagcttaaa aatgtttcag cttcggtgcg gcaacgcctg ctgaaccatt cccgcgaaag
     8881 acatcagccc ttcggtcagg tggttcagta ctacgcaatg gagcgctttc tgtacaggct
     8941 tggcagatcc gcgctggtgg accgcttcat tctcaaaggc gccctgatgc tccaggtgtg
     9001 gcactcgccg caatcccgcc cgaccatgga catcgacatg ctcggcagaa ccagcaacga
     9061 cgaagccgcc gttgtggccc agattcggga aatcatctcc accgacgtgg agccggacgg
     9121 aattcagttc gacccggatt cgatccgcac ggaagacata accagggaag ccgattacga
     9181 cgggatcagg atacgttttc aaggcaaact ggattcagtc agaatcaaca tgcaaatcga
     9241 tatcggattt ggcgatccgg tattccccgg ccccgagatg gcagaactcc cgacgctgct
     9301 ggactcgccg acgccgaggc ttttttgcta cagccgggaa agcgccatag ccgaaaaatt
     9361 tcatgcaatg gtgattcgtg gccacctcaa cagtcgcatg aaagactttt ttgacatctg
     9421 gctgttatcc aggtccttcg cctttgacag tgaaaaactg gccaaggccg tgcggctgac
     9481 atttgactat cgtcacacag cggtgccgga aaatctcgaa gtgtttgacc cagcttttgt
     9541 cgcgtcaaaa acagggcaat ggacagcgtt cagaaagaaa ttcagtcatc tgcacatacc
     9601 cgaatcgttc gacgtcatcg tgctggatat cagcgatttt ctacgtccgg taatagcggg
     9661 cggcgatgcg tcatcatcgc caaaacactg ggaaccacaa ggtccctggc gcgattgaac
     9721 ctctgcttcc aaacgacgtt acttcaacga ataccacacc tgcgcgtcac ccggccgtcc
     9781 agacttgtac acacgaagtg gcgaggcgtt cgacagcccc ataccctgcc gccatgaaaa
     9841 tcagaaccat ttcaaaattt gccgtcttat caatcagtta ccttcgttcc tggttccgtt
     9901 tcagaaacga ctggccatgg aaaacctggc gcagaggcaa cagttggcga tgttcaagca
     9961 ggctcggccc cggccgatgc cgacggatgc cgaccgtgtc ttctgggttc tgctgcggga
    10021 aatgtgggcg aagtggagcg acacgctgat cgtggtgaca ccggacacga ttgtacggtg
    10081 gcaccgggaa gggttccggc ggtactggaa tgcctttccg aaacccagga agtcaaattt
    10141 ttcgtcgaca acctgatcaa aaatgatatc gcattttggt aaaatggcct ccgatccaat
    10201 tttggcgagg gactggacag tacgatggtg aagatcaaat ggacaatgat gattgcaaca
    10261 gccttgacgt cggcactgat attaacgatg gggtgtggcg atgacacgca tggagtcaac
    10321 gatacggacg acgtagttga tgcggcggat gccgaagatg cgacagggct cgacgggatg
    10381 atgttcgtcc cttccggttc attctggatg ggttgtaacg acgcagcagc caacaattgt
    10441 aatcctcagg aaaggcctta tcacctggtc accttagaca gcttctatgt ggacaggact
    10501 gaagtgacga tcaaggattt ccaggtttgc gtggacgccg gcgtgtgcag ccatcacctg
    10561 gacgaccaga catgttttta cataattctc gaaaacggcg atctggcaca aagcggaata
    10621 tggccagcat ttcgcggcac caaccaaccg atggtctgcg tcgactggga acaagcgaaa
    10681 cagtattgcg aatgggctgg caagcgtctg ccgacagagg cccagtggga aaaggccgcc
    10741 cgaggaactg atggacgcgt gtatccgtgg ggaaacgaca atccagactg caataacgcg
    10801 gtcacgtatg ttgacgatgg ctccgaatgc gctccagaag ttaccgccga tgtctgcaac
    10861 aagtcaccgg ctggtgacag tccttacggt ctgtgtgaca tggccggaaa cgtcagggag
    10921 tgggtcgcag actggtacga ctccaactac tatgacacaa gcccgaccac taatcccaaa
    10981 ggcccagaca ccggattgta tagaaccatt cgtggcagta gtttctatcc gggaaatgat
    11041 ctcaccaact ctggccggct tggtgcagag gcatccaagg cattcgtcga tcgcgggttt
    11101 cgctgtgttt ggtcagcaga gagttcaaat caatgaagac gccgccctgg tcagaacatg
    11161 ctcgctatct tgtggctgaa tcggacacct ccccaaaccc gcccatggaa gacggaattc
    11221 tgtctccatt ttcgtctgcc agtggccgaa ttttggtgat tttccattga acgaagggca
    11281 aaaaacgtaa agaaatcaaa gggtctctgg tggaggtaga cggaatcgaa acacgactgt
    11341 ccgccaaatg acaacggcgc ttgcgccggt aagcgcctga cgcacgggcg cgcgcgttta
    11401 cgcgcttcat ggacctcttg aatgccatcg agagatcaac ggtatcattt cgggacgtta
    11461 caaaacaaag cgcagcacac agcgaagatt atcacgaaat ttcagggagt tatggcggat
    11521 taccgttttc cgccctattt cgtcgcccag aacacgctgt ttttccagtc aactggaatt
    11581 cccatggctt tgcgccacct gggctcggca tcccaacgcc ccagaaggcc aactatctgc
    11641 cgtctccatt tatctccgca gggctcatca atcgacagat gtcttaagat gcacaacacc
    11701 ccgtatacct ttttgggatc aggtatcgca ggcggtttcc acgctgcctc atagggaaat
    11761 ttcggcgcaa ccgacaattc ccggttccag aggcgggagt ggtgggcgca aatgtttctg
    11821 acaaacgcca gtgtgtgtag ccacgactga acgacaacct tgttgacgcc aaagggtctg
    11881 gcaagtacag cctgatcgga accgttcaga cccttgtaca tgaatgaaag tgttccaaac
    11941 gacatcactt ccgcagccac ccagacctgt tgccgctcgc agtaaaacgt aaaaatctgg
    12001 acatc
//
LOCUS       DAOPPA010000028        11905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6201_length_11905_cov_4.693671, whole genome shotgun sequence.
ACCESSION   DAOPPA010000028 DAOPPA010000000
VERSION     DAOPPA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_11905_cov_4.693671"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKH54_01675"
     CDS             <1..338
                     /locus_tag="PKH54_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98625.1"
                     /translation="PPVVVAPAPVVQEPAPAPVAEPPAVIEPAPAPAPVAPAPAPVAA
                     AQSGPKPNCQKVCEKFVQAVSIESARPPMFSACFNRCDSGDMGYSKCAWMAATMADVV
                     NCNKMPAAK"
     gene            complement(339..1892)
                     /locus_tag="PKH54_01680"
     CDS             complement(339..1892)
                     /locus_tag="PKH54_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98626.1"
                     /translation="MMERFFQSKRGVVVAVALVMASCLFRLWFVPDARLAGDEATQYS
                     TAMQVADLKLFPVKGVNMTGDAAMTPGGLYHLIMSIPFFFTDDPRGAGVFLVLLNCAS
                     MLFGWFILRREFGGVAATFGLIISLFNPFSVFFSDRQWNPNLLVPLGWLWIWLLLSAF
                     RGEGRFRWGWLATLLVVSPQIHMSCPHLTIMTVVMMAVFRPRIRPLQIAGGTAIGMAT
                     YVPYLAVDAYNGWSNTTRILNQLSEAAAPWYEAFRGGLYQLLYAGGDYTYFLGKGFWF
                     PMTEWGFLGSGGSTAYSTLLGFPSWPGYPALALLCAALAVAAAAHAFVLLDAVRRGSR
                     GVTAFIRSEPLTVLAILNIPVLILLMLKTSKPFYPHYSMVLFPLAIVPVAAAVSRLGR
                     SRTLVAGLMVLAAAIALNQAILTARYYRAEESKASVHLVVEASRTILADAGPSSFRLV
                     FDMPGTRMGTWPFATVARVLHKAEWRERKDGTRLTYVIAPPGSRHEARAARIWDIDGK
                     RLIRKEDPK"
     gene            complement(1967..2740)
                     /locus_tag="PKH54_01685"
     CDS             complement(1967..2740)
                     /locus_tag="PKH54_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98627.1"
                     /translation="MKKSIFGRLLAVGVLAASLPLLVTCEVPNPRALFIYDAQAITPR
                     SQCIIQPGQRAQAVLAFGILDSMLTNNYFLFPRFRNMMMKSTTISGEGIESGNVETNY
                     LNISGAKVWIDLGQLGDELQATGRMTEEQLFILSTDGVQHTVAAGAEPEQEGTVGVEV
                     IKTELGNLIGEVMRTHAGEEYGADINVYVMLMAENQAGEIIYSNELAFPIKVCFGCLV
                     RPSADDTITDPPCFPGQDYGVPAVICPAIAEYPTFCPVY"
     gene            complement(2737..6969)
                     /gene="pilQ"
                     /locus_tag="PKH54_01690"
     CDS             complement(2737..6969)
                     /gene="pilQ"
                     /locus_tag="PKH54_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOC98628.1"
                     /translation="MSSAKLTGLLSATAFILISGVATATPDDANRISGITVREEPEET
                     VITIAAERTPTFSVFKLEEPVRLFVDVPQGDISKVADGVMVDNGVLDRVATMQIKSNG
                     TPVGRIILGLRQSAAYDIKAIDRGIVIRIDGAGRRPSQEDGKVLAEQVDKIRAELARE
                     KELLEKIRETRERDSKLRLTEEQSRLEAERLRAKAEELKKAAEVEAREAGRLAAEEKT
                     RLEETRKARIAEEKTRGQLTELARLEAAKLDDLRRSRENEEKLVAQLAQARNQNVSAK
                     VSEIEARLEKARAEKASGEKEIAALRTKIDLARTEATKASDLAREAERLQASVVAEKQ
                     KLEKLTRDSQEASKAWSEATGRLKTEELRLRAIAQRREAAEQEVAALRAEVDNLKSAR
                     KAVETAEARKLREQMESRESELKELRARVEKAGEVADAATIRLAAKEKEISDLKAGLE
                     GQRRTLQQKLEAEKAAATAALSSREREIRELRDAAEKARAAAAASDGRLTESQKQAIA
                     KFQAKEKELQAARVEAEKTARNAEATLARERGQAEARMAAREKELESLRAAMIAAKKA
                     AEEEGRSKALAQSEAEVRKLREKYEGALADVRETNRKQEQTIRDLSRQVASAQEDADQ
                     ARREIALKEQKISSLTSELAQLQQVSSRQEKAEVSRLSKLVAEREAELKRVNRAKASG
                     AAKDRQVATAAASLREARDMMSAAERREREAVAREQAAQARIAELTAVAGSSEDRLAQ
                     KQKEVERLNSELAAARTALDSAAESGRRELSEQIDRLTAELAQAREAENRTARQANAA
                     AQAEIDRLNAELAKAKAAGRAATGVASVKAIDFKKIGSVPSVILTLDGSADYSVSSRD
                     GAYVLTVSGASLPKELARRLDVTAFKTPVNMVSSFATENGTIQIVAELSGQVGQEIAR
                     RDGKLVWTFSGPAPADMFASSQPVPRVGRTVAQATPPPAPAAPSSSPEMAEDPSAQPQ
                     GYLRPSMVPKKKKYQGKRINLTVKDADMQNVLTFLAREGKVNIVTSEEVKGKVTFHLE
                     DVPWDLALDTVLKAKGMDYVVEQGIFRVASAESIQKEYEAKVEKAKKQRELKPVVVRL
                     VPVNYGRGQEMATRLKDVLSDKGSVSVDERTNTLVIKDTEDYLQAAEDLVKRLDQQTP
                     QILIEARIVEARTTFSEDIGIQWGGNFAMNSAYGNETGLVFPSSLGLAGGATPADPSG
                     GLTLDNPNWAVNLPAAVGTGSGGAIGIQLGSIGGIGNLTLRLSAAEETGDVKIISSPR
                     ISTLDNRKASISQGVQIPISVVSAAGVNTQFFSADLKLDVEPHVTRDGHINLKLDISK
                     NEPDFGNLAANGNPTIQKKEAHTELLIRDGDTTVIGGIYTRSTGKSFKKIPFLGDIPV
                     LGWLFKSRNRSDDRSELLIFITPKVVNREVSL"
     gene            7364..8527
                     /locus_tag="PKH54_01695"
     CDS             7364..8527
                     /locus_tag="PKH54_01695"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC98629.1"
                     /translation="MQKAEYEVIPFPKSNLPAPSRSIEGLKPYDAVSSLDAISRLPAG
                     LSPLKLDWNESVIPPSPRVVDEITKFLRNGHHINWYPDLGASKLRRTLAAYCGVDSED
                     IIVTNGSDDALSLICSSYLDPEDDVVVVWPTYGHFVIFVKGRGVEPRMAAPEDPFDNP
                     TETIRRTIRQDTKLVYIASPNNPTGVVTPPEDVQALCREFPSTLFVVDEAYYEFCRVT
                     SAWLVGKMPNLIVTRTFSKCLSMAGLRIGYLMASQEVISNLKRLYNPKSVNVLGQVGA
                     AAALMDYEFISRYVNDCRAARSWLVQELAARGADVRNTQGNYILVRVKDPAGFCGRLE
                     SVGVFVRDRSFIEGFEGYVRITVGPLDYMKEFVRRIDMLLEKNPDLMAPWSIA"
     gene            8618..9082
                     /gene="ribE"
                     /locus_tag="PKH54_01700"
     CDS             8618..9082
                     /gene="ribE"
                     /locus_tag="PKH54_01700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOC98630.1"
                     /translation="MARVLEGKLTARGLRVALIVSRFNHFIVDRLEEGAKDALARHEA
                     DEKDIDVIRVPGAFELPLIAQKAAKSGRYDAVICLGCVVRGSTPHFDYVASEAAKGIA
                     MASMDSGVPVIFGVLTTENLEQAIERAGTKGGNKGWSAAMSAIEMANLIKQM"
     gene            9099..9563
                     /gene="nusB"
                     /locus_tag="PKH54_01705"
     CDS             9099..9563
                     /gene="nusB"
                     /locus_tag="PKH54_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOC98631.1"
                     /translation="MGTRRRGRECALQLLYMWEYHRDGGFDFNTFWIELPQEEKATRQ
                     FAERLVDGVTSNLDHIDGLIKDASTNWRIDRMANVDRNILRLATWELENISETPLKVV
                     LNEAIELSKRFGSEDSSAFVNGVMDRIGSLLRPGALRDQEEPDPARPQPVEG"
     gene            9569..10111
                     /locus_tag="PKH54_01710"
     CDS             9569..10111
                     /locus_tag="PKH54_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOC98632.1"
                     /translation="MKVSLVKPDLHAIDQLDIDTVAIPVFEDMRPPAGLTGLIDWRLC
                     GRVSALLVSGQLSGRFREAAMMPGYGRLPGTRIVAFGMGDSREFSQSRAREAAWFMSD
                     SLRRLKTGAFLTALPGSMSKTVPPRAGLEMMIEEIGRVFSADESFQSLDVSIVEAQES
                     HRELNDLVSSAMRRLRGVWK"
     gene            10145..10837
                     /gene="pyrF"
                     /locus_tag="PKH54_01715"
     CDS             10145..10837
                     /gene="pyrF"
                     /locus_tag="PKH54_01715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOC98633.1"
                     /translation="MIPAENICVALDTADLGRARELASALAREVGWLKVGLTLYGACG
                     PEAVKALSGFGPKVFLDLKLHDIPVQVAGAVRAASDLGAGLLTVHASGGPAMMKAAAD
                     GRANGLKVVAVTVLTSISEAELDAVWPGSTPAGAVARLTSMAVDAGLDGVVMSPLEVS
                     RMRSVVPPEFMFVVPGIRPAGKPGTTGDDQSRVGTPGEVYAAGASVLVVGRPITGAAD
                     PVAATRLVRGIA"
     gene            10882..>11905
                     /locus_tag="PKH54_01720"
     CDS             10882..>11905
                     /locus_tag="PKH54_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOC98634.1"
                     /translation="MGQERKDRPSGRQARDGQATGSRRPAREGGASGSGRQPREGYGA
                     GSGRPPAEGRGHGTGRSPAADRFRGESRPGREQGRAGGFGSWQRRDETSPGRSKGFDA
                     RKDPRLDDQMVVYGIHPVSEFITVRPKQIRHILLDRRTPPGAIDAARRAAGLGIPVRE
                     AAAGEFEELVGPANAQGIVAILRPMEPVDGDDLIDSVLAAGEGLLVALDSIQDPQNLG
                     SILRTAAFFGAAGVIITKDRSVRLTSSAVRASAGGAARVPVGEVTNLARALGRCVDAG
                     MTVVGTVVSRGQPLSEVPASGPRVIVLGSEGALEGARAGSILVDMSSISPLVAQKVGA
                     ACAAKGV"
BASE COUNT         2090 a         3595 c         3684 g         2536 t
ORIGIN      
        1 ctccgccggt cgtggttgcc ccggcaccag tcgtgcagga acctgccccc gcgccggtcg
       61 cggaaccgcc ggccgtgatt gagccggccc ccgcgcccgc gccggtggca cccgctcccg
      121 ccccggttgc cgcggcacag tccggcccga agccgaactg ccagaaggtc tgcgaaaagt
      181 tcgttcaggc ggtgtcgatc gaatcggcgc gtccgccgat gttcagcgca tgcttcaacc
      241 gttgcgacag cggtgacatg ggttattcga agtgcgcgtg gatggccgcg acgatggccg
      301 acgtcgtcaa ctgcaacaag atgccggccg cgaagtgatc attttggatc ttcctttcta
      361 atcaatctct ttccgtcgat gtcccatatc cgcgcggccc tggcttcgtg cctggaaccg
      421 ggcggcgcaa ttacgtaggt cagcctggta ccatccttcc gttcgcgcca ttcggctttg
      481 tgaagcacgc gggccacggt tgcgaatggc catgtcccca tccttgtccc tggcatgtcg
      541 aaaacaagcc tgaacgatga agggccggca tccgcaagga tggttcttga ggcctcgacc
      601 acaaggtgaa cggatgcctt cgattcctcg gcgcgatagt accttgcggt aaggatggcc
      661 tggttgagcg cgatggccgc cgccaggacc atgaggccgg cgaccagcgt ccttgatcgg
      721 cccagccggg aaacggccgc cgcgacggga acaatcgcca gcgggaaaag aaccatggag
      781 tagtgcgggt aaaacggctt ggacgtcttc agcatcagca ggatcagaac cgggatgttg
      841 aggattgcca gcacggtcaa aggttctgac cggatgaatg ccgtaacgcc ccgagagccg
      901 cgtcgcacgg catcgagcag cacgaatgcg tgggcggcgg ccgcgaccgc aagagcggcg
      961 cacagcaggg ccagggcagg gtagccaggc cacgacggaa atccgagcag cgtcgaatag
     1021 gcggtcgaac ccccgcttcc aagaaagccc cattccgtca tcgggaacca gaagcccttg
     1081 ccaaggaaat aggtgtagtc accgccggcg tacagcaact ggtaaaggcc gccgcggaag
     1141 gcttcgtacc acggggccgc agcttcggaa agctggttca gaatcctggt cgtgttcgac
     1201 catccgttgt aagcatcgac ggccaggtac gggacatagg tggccatgcc gatggcggtt
     1261 ccaccggcaa tctgcaatgg ccggatccgt ggcctgaata cggccatcat gaccaccgtc
     1321 atgattgtaa ggtgcgggca ggacatgtgt atctggggag acaccaccag cagcgtggcc
     1381 agccagcccc accggaaccg accttcaccg cggaacgcgg aaagcagcag ccagatccac
     1441 agccatccaa gaggaacaag aaggttcggg ttccattgac gatccgagaa aaagaccgaa
     1501 aacggattga acagcgagat gatcagcccg aacgtagcgg caacgccccc gaattcgcgt
     1561 cgcaggatga accagccgaa aagcatcgat gcgcagttca gaagaaccag gaaaacgccg
     1621 gcacccctgg ggtcgtccgt gaagaagaac gggatcgaca tgatcaggtg gtacagcccg
     1681 ccgggcgtca ttgcggcgtc gcccgtcatg ttgacgccct tgacggggaa aagcttcagg
     1741 tcggccacct gcatcgccgt tgagtactgg gttgcctcgt cgcccgccag gcgcgcgtcg
     1801 ggaacgaacc agagcctgaa caggcatgag gccatcacga gagcgaccgc gacgacaacg
     1861 cccctttttg actggaagaa ccgttccatc atgattccag ttcagttacg cgtgaaggat
     1921 atcaccggaa gcgacatgat gtggatttcg ctgcttcgat gcgggatcaa tagaccgggc
     1981 agaaggtggg gtattcggcg attgccgggc agatgactgc cggaacgccg tagtcctgtc
     2041 ccgggaagca aggcggatcg gtgatcgtgt cgtcggccga cggtcttacc aggcatccga
     2101 agcagacctt gatcgggaac gccagttcgt tcgaatagat gatttcgccc gcctggttct
     2161 ctgccatgag catgacatac acgttgatgt cggcaccgta ttcctcgccc gcgtgagtcc
     2221 tcatgacctc gccgatcagg ttgcccagct ccgtcttgat tacctcgacg ccgaccgttc
     2281 cttcctgttc aggttcggcc ccggccgcga ccgtgtgctg gacgccgtcg gttgagagga
     2341 tgaaaagctg ttcttcggtc atccggcccg tggcctgaag ttcgtcaccg agctgcccaa
     2401 ggtcgatcca taccttggcg ccactgatgt tcaggtagtt cgtttccaca ttgccggatt
     2461 cgatcccttc cccgctgatg gtggtgcttt tcatcatcat gttgcggaat cgcgggaaca
     2521 ggaagtagtt gttggtcagc atcgaatcca ggatgccgaa cgccagtacc gcctgggccc
     2581 gctggccggg ctggatgatg cactgcgaac gcggcgtgat ggcctgggca tcatagatga
     2641 acagggcccg cgggttcggc acctcgcacg ttacgagcag gggcagggac gctgccagta
     2701 cgccgacagc aagcagtctg ccgaaaatgg acttcttcat aaggacacct ctctgttcac
     2761 gaccttcggg gttatgaaga tgagcagttc tgaccggtcg tcagaacggt tacgggactt
     2821 gaacagccag cccaggacgg ggatgtcgcc aaggaacggt attttcttga acgacttacc
     2881 cgtgctacgc gtatagatac cgccgatgac ggtcgtgtcg ccgtcccgga tcagcagttc
     2941 cgtgtgggct tccttcttct ggatggtcgg gttgccgttg gcggcaaggt taccgaagtc
     3001 gggttcgttc ttgctgatgt cgagcttgag attgatgtgt ccgtcacgcg ttacgtgcgg
     3061 ctcgacatcc agcttcaggt cggccgagaa gaactgcgtg ttgacgcccg ccgccgaaac
     3121 gacgctgatc gggatctgga cgccctggct gatcgatgcc ttgcggttgt ccagggtcga
     3181 gattcggggc gaactgatga tcttcacatc gcccgtttct tccgcggccg acaggcgcag
     3241 cgtcaggttg ccgattccgc cgatcgaacc cagctggatg ccgatggcac cgccggaccc
     3301 ggtgccgacg gccgccggga ggttgaccgc ccagttcggg ttgtcaagcg tcaggccgcc
     3361 gctggggtcg gcaggagtcg cgccgccggc cagaccgagg ctggacggga acacaaggcc
     3421 ggtttcgttt ccataggcgg aattcatcgc gaaattgccg ccccactgga tgccgatgtc
     3481 ttcactgaag gtggtccttg cctcgacgat tcttgcttcg atcaggatct gcggggtctg
     3541 ctgatcaagc ctcttgacca ggtcttcagc cgcctgcagg tagtcttcgg tgtccttgat
     3601 gaccagcgtg ttggttcttt catcgaccga aacggagccc ttgtcggaaa ggacatcctt
     3661 cagcctggtc gccatttcct gaccgcggcc gtagttgacc gggacaagcc tgacgacgac
     3721 cggcttcagt tcgcgctgct tcttggcctt ttcgaccttg gcttcgtatt ccttctggat
     3781 cgattcggca gatgccaccc ggaagatgcc ctgctcgacg acgtaatcca ttccctttgc
     3841 cttgagcacg gtatcgagcg ccagatccca cggcacgtct tcaaggtgga aggtgacctt
     3901 gcccttgact tcttcggatg tgacgatgtt gaccttgcct tcacgggcaa ggaacgtcag
     3961 cacgttctgc atgtcggcgt ccttgaccgt aaggttgatg cgcttgccct ggtacttctt
     4021 cttcttcgga accatcgacg ggcgcaggta tccctggggc tgcgccgaag gatcctcggc
     4081 catttccggg cttgatgacg gtgcggccgg ggccggtggc ggcgttgcct gggcaacggt
     4141 tcgtccgacg cggggaaccg gctgggacga cgcaaacatg tcggccggtg ccggtcccga
     4201 gaaggtccac accagcttgc cgtcgcgcct ggcgatttcc tgtccgacct ggcccgaaag
     4261 ttcggcgaca atctggattg ttccgttttc cgtggcgaaa gagctgacca tgttgacagg
     4321 ggtcttgaac gccgtcacat caaggcgcct ggccagttcc ttcggaaggc tggcgccgga
     4381 aaccgtaagg acataggcgc catcacggga cgaaaccgaa tagtcggccg agccgtcaag
     4441 cgtcagaatc acgctcggca ccgaaccgat cttcttgaaa tcaatagcct tgaccgaagc
     4501 gactccggtc gctgcgcggc cggccgcctt cgccttcgcc agttcggcgt tcaggcgatc
     4561 tatttcggcc tgggccgcag cgttggcctg tctggccgtg cggttctctg cctcccgcgc
     4621 ctgcgccagt tccgcagtca ggcggtcaat ctgttccgaa agttccctgc gtccgctttc
     4681 cgccgcgctg tccagcgccg tgcgggccgc agcgagttcg gaattgagcc tttcgacttc
     4741 cttctgcttc tgggccagcc tgtcttcgga agaaccggcg acagcagtca gctcggcaat
     4801 tctggcctgt gcggcctgct cgcgagccac ggcttccctt tcgcgccgct cggcagcgga
     4861 catcatgtcg cgtgcctcgc gcagggaagc ggccgccgtc gcgacctgcc tgtcctttgc
     4921 ggctccagac gccttcgcgc ggttgacgcg cttcaattcg gcctcacgtt ccgcgaccag
     4981 cttgctcagg cgcgaaacct ccgccttttc ctggcgtgac gacacttgct ggagctgggc
     5041 aagttccgag gtcagcgacg atatcttctg ctccttaagg gcgatctccc tgcgggcctg
     5101 atccgcgtct tcctgggcgg atgcaacctg gcggctgaga tcacggatgg tttgttcctg
     5161 cttgcggttc gtttcccgca catcggccag ggccccttca tatttctcgc gcagtttacg
     5221 aacctcggcc tcgctctggg ccagcgcctt gctgcggcct tcttcctcgg ccgccttttt
     5281 cgcggcaatc atggcggcgc gcagggattc cagttccttt tcccttgcgg ccatgcgggc
     5341 ttcggcctgg cctctttcac gtgcgagcgt cgcttcggcg tttctggccg tcttctctgc
     5401 ctcgaccctc gccgcctgaa gttccttttc ctttgcctgg aatttcgcaa tcgcctgctt
     5461 ctgcgattcc gtaagccgtc catcggacgc agccgcggcc gcccgggcct tttccgccgc
     5521 gtcgcggagt tcccttatct ccctctccct ggatgacagc gccgcggtcg cggccgcctt
     5581 ttccgcctcg agcttctgct gaagcgtcct gcgctgtcct tcaaggcctg ccttcagatc
     5641 gctgatttcc ttttccttgg cggcaagccg gattgttgcc gcgtctgcca cctcgccggc
     5701 cttctcaacc ctggcccgca gttccttcag ttcggattcg cgcgattcca tctgctcgcg
     5761 cagtttcctc gcctcggccg tctcgaccgc cttgcgggcg gacttcaggt tatcgacttc
     5821 ggcgcggaga gccgccactt cctgctcagc cgcctcgcgt cgctgggcaa tcgccctcag
     5881 ccgcagttct tcggtcttca ggcggcctgt cgcctcgctc caggccttcg atgcctcctg
     5941 gctgtccctg gtcagcttct caagcttctg cttttcggcg accacgcttg cctgaagcct
     6001 ttcggcctcc ctggcaaggt cggatgcctt ggtggcctcg gttctggcca ggtcgatctt
     6061 tgtcctgagg gccgcaattt ccttttcgcc cgaagccttc tcagcccggg ccttttccag
     6121 cctggcctca atttccgaca ccttggcgct tacgttctga ttgcgggcct gcgccagttg
     6181 cgcgaccagc ttctcctcgt tttcccggct cctgcgcaga tcatcaagct tcgcggcctc
     6241 gagcctggcc agttcggtaa gctggcccct ggtcttttcc tcggcaatcc gagccttcct
     6301 ggtctcctcg agcctggtct tttcctccgc ggcgagtctg cccgcttccc gggcctccac
     6361 ctcggcggcc ttcttgagtt cctcggcctt cgccctcagg cgctcggcct caaggcgact
     6421 ctgttcctcg gtcaggcgca gcttggaatc gcgttcgcga gtctcgcgaa tcttttcaag
     6481 cagttccttc tcgcgcgcca gttccgcccg gattttatcg acctgctccg ccagaacctt
     6541 gccgtcttcc tgcgaaggtc ggcgaccggc accgtcgatg cgtatcacaa tcccgcggtc
     6601 aatcgccttg atgtcgtatg cggcgctttg cctcaggccg aggatgattc ggcccaccgg
     6661 tgtcccgttg ctcttgattt gcatcgtcgc gacccggtca agaacaccgt tgtcaaccat
     6721 gacaccgtca gccaccttgc tgatgtcgcc ctgcgggaca tcgacgaaaa ggcgaaccgg
     6781 ctcctccagc ttgaagacgc tgaaggtcgg agtcctttcc gcagcgatgg ttatgacggt
     6841 ctcctcgggc tcctccctga cggttatccc ggaaatccgg ttggcatcgt caggcgtggc
     6901 ggttgccacc cccgatatca gaataaaagc tgtggccgac agcagccctg taagtttcgc
     6961 gcttgacatc gcgacctcca cctgaaaagg caaacagttc cgttgaaatc gtaagcccgg
     7021 atcttcaaac ccgtcaaaga aacagcagtt gttttttgca gaacccgttt tttgtcgcga
     7081 agtcgtcgcg actggggcat gtcccctatt aatgatattg cgacttcagt gcgcggtcgt
     7141 ccggaaggca tcgatggcga attcgtagtc agatgtggag tatgttttat ccagtagatg
     7201 gcggaaccct gtcgtccatt tggataagat gcggattttg ttgtatttca aagtttgatg
     7261 cttctggcgt ggattttgca tgtacaattc gcgccccggc tttccggccc tgatactcca
     7321 ggcccggtca tttgccgaat ttcataattg aaggtgactg caattgcaga aagctgaata
     7381 cgaagtaatc cccttcccga agtcgaacct tcccgcgccg tcgcgttcga tcgaaggttt
     7441 gaagccatat gacgccgttt cctcgctgga cgcgatttca cgtcttcccg ccggcctgag
     7501 cccgctgaag ctggactgga acgaaagcgt gattccgccg tcgccccggg ttgttgacga
     7561 gataacgaag ttccttcgca acggacatca catcaactgg tatccggatc ttggcgcatc
     7621 caagctccga agaaccctgg cggcctattg cggtgtcgat tccgaggaca taatcgtaac
     7681 caacggttca gacgacgcgc tgagcctgat ttgttcttcc tatctggatc ccgaggacga
     7741 cgttgtcgtt gtttggccga cttatggtca tttcgtgatt ttcgtcaagg gacgcggcgt
     7801 cgagccgagg atggcggcac cggaagatcc tttcgacaac ccgaccgaga ccatccggag
     7861 aaccatccgc caggacacga agctcgtcta tatcgcgagt ccgaacaatc cgaccggcgt
     7921 ggtgaccccg ccggaggacg tgcaggccct gtgcagggag tttccttcga ctctgttcgt
     7981 cgtcgatgag gcctattacg agttctgccg cgtgacttcg gcctggctgg tcggcaagat
     8041 gccgaacctg atcgttacaa gaaccttctc gaagtgcctc agcatggccg gcctgcggat
     8101 tggctacctg atggcatccc aggaggtcat ttcgaacctg aagcgccttt acaatccgaa
     8161 gagcgtcaat gtgcttggcc aggtcggcgc cgccgcggcc ctgatggatt acgaattcat
     8221 ctccaggtac gtgaacgact gccgtgccgc gcgttcgtgg ctggttcagg aactcgcggc
     8281 ccgcggggcg gatgtccgca acacgcaggg caactacatc ctggtcaggg tcaaggatcc
     8341 ggcaggattc tgcggcaggc tcgaatctgt cggcgtgttc gtccgcgaca ggagtttcat
     8401 tgaagggttc gagggctatg tgaggattac ggtcggtcct cttgattaca tgaaggagtt
     8461 cgtcaggaga atcgacatgc tcctggagaa gaaccccgat ctgatggcac cctggtccat
     8521 cgcgtgacgc ggtcgcgctt gttcccgcgc tattgttaat caccaccttt tgagttaaat
     8581 tgttgcggca ttgattgatg tgccgggagg ttcataaatg gccagggtac tggaaggaaa
     8641 attgactgcc aggggtctca gggtcgccct gattgtaagc cggttcaacc atttcatagt
     8701 cgatcgcctc gaggaaggcg ccaaggacgc cttggcaagg cacgaggcgg atgagaagga
     8761 tatcgacgtg atccgcgtcc ccggcgcgtt cgagttgccc ctgattgcac agaaggccgc
     8821 gaagtctggc cgatatgatg cagtcatctg ccttggctgc gtggtgcggg gatcgactcc
     8881 gcattttgac tacgttgctt ccgaggcggc aaaaggcatc gcgatggcat cgatggactc
     8941 cggcgttccc gtcatcttcg gcgtgcttac gaccgaaaat cttgagcagg caatcgagcg
     9001 ggcagggacg aagggcggaa acaagggctg gagcgcggcg atgtccgcaa tcgaaatggc
     9061 caatcttatc aagcagatgt aactgtcccg gggggccgat gggtacaagg cgacgcgggc
     9121 gtgaatgcgc ccttcagctt ctttacatgt gggaatatca ccgcgacggc ggttttgatt
     9181 tcaacacctt ctggatcgag ttgccccagg aggaaaaggc gaccaggcag ttcgccgagc
     9241 ggcttgtcga tggtgtcacg tccaacctcg atcatatcga cggcttgatt aaggacgcat
     9301 cgaccaactg gcgaatcgat cgcatggcaa acgtcgatcg caatatcctg cgcctggcga
     9361 cctgggaact cgagaacatc tcggaaacgc ccctgaaggt ggtgctgaac gaggcgatcg
     9421 agctttcgaa acgtttcgga tccgaagact ccagcgcttt cgtcaacggc gtcatggatc
     9481 gtatcggttc cctgctccgg ccaggcgcgc tgcgtgacca ggaggagccc gatcctgcac
     9541 gaccccagcc ggtggagggc tgacgtccat gaaggtatcg ctggtcaagc cggatctgca
     9601 cgcgattgat cagctggata tcgatacggt cgccattccc gttttcgaag acatgcgtcc
     9661 gccggccgga ttgaccgggc ttatcgactg gaggctctgt ggcagggttt cggcgcttct
     9721 ggtgtccgga cagctttccg gaaggtttcg cgaggcggca atgatgccgg ggtacgggcg
     9781 gctgcctggc acaaggattg tggctttcgg catgggcgac agccgtgagt tttcccagtc
     9841 ccgggcaagg gaagcggcct ggttcatgtc cgattcgctt cgacggttga agacgggcgc
     9901 tttcctgacg gcccttccgg gctcgatgtc aaaaacggtg ccgccgcgtg ccgggcttga
     9961 aatgatgatt gaggagatcg gccgggtctt ttccgcggac gagtcttttc agtcgctgga
    10021 cgtaagcatc gttgaagcgc aggaaagtca tcgcgagctt aacgatctgg tctcctcggc
    10081 gatgaggcgt cttcgcgggg tttggaagta atccgttcgt ttcactcatt caggatttca
    10141 cgaaatgata ccagccgaaa acatatgtgt tgcactggac acggccgatc tgggtcgagc
    10201 gagggagctt gcgtcggcac tggctcgcga ggtgggctgg ctgaaggtcg ggcttacgct
    10261 ttacggcgcg tgtggtcccg aggccgtcaa agcgctttcc gggttcggtc cgaaggtgtt
    10321 cctggatttg aaactgcacg atattccggt tcaggtcgct ggtgccgtca gggccgcctc
    10381 cgatcttggg gccggattgc tgaccgttca cgcctcgggc ggccccgcga tgatgaaggc
    10441 ggcggccgat ggccgcgcca acggattgaa ggtggttgcg gttacggtgc tgacttccat
    10501 ttccgaagcg gaactcgatg cggtctggcc tggttcgacc cccgcggggg cggttgcccg
    10561 cctcacctcc atggccgttg atgccggcct ggacggcgtc gtgatgtcac cgctggaagt
    10621 ttccaggatg cgatccgtgg ttccccctga attcatgttt gtggttcccg gaatccggcc
    10681 tgccgggaaa cccgggacaa ccggcgatga tcagtcccgc gtgggaaccc ctggcgaggt
    10741 atatgccgcc ggcgccagcg tgctggtggt cggacggccc ataaccgggg cggccgatcc
    10801 ggtcgccgcg accagacttg tgcgtggcat tgcgtgattt tcggcatcgg ttttcaggaa
    10861 atgtaacgga aggcggaaaa attgggacag gaaaggaagg atcgcccatc gggaaggcag
    10921 gcaagggatg gtcaagccac tggatcgcgg cggccggcgc gcgaaggcgg ggcttccgga
    10981 tcagggcggc aaccccggga aggatacggc gccggatccg gcaggccgcc ggcggaaggt
    11041 cgtggtcatg gaaccggaag atccccggcg gccgacaggt tccggggtga gagtcgtccc
    11101 ggccgtgaac agggccgggc aggcggtttc ggatcctggc agcgtcgcga cgagacttcg
    11161 ccggggcgat cgaagggctt cgatgcccgg aaggatccca ggcttgacga tcagatggtc
    11221 gtgtatggaa tccatccggt ttcggaattc ataacggttc gtccgaagca gataaggcac
    11281 atcctgcttg accgcaggac gccaccgggt gcaatcgacg cggcaaggcg cgcggccggt
    11341 cttggaattc cggtgcgcga ggccgccgcc ggcgaattcg aggaactggt cggtcccgca
    11401 aatgcccagg gcatcgtcgc catcctgcgt ccgatggagc cggttgacgg cgacgaccta
    11461 atcgattccg tcctggcggc cggcgaagga ttgctggtcg ccctggattc gatccaggat
    11521 ccccagaacc ttggttcgat cctgcggact gccgcatttt tcggtgccgc cggggtcatc
    11581 atcaccaagg atcgcagcgt caggcttact tcgtcggcgg tgcgggcgtc cgccggcggc
    11641 gcggcccgcg ttcccgtggg cgaggtgacg aaccttgcgc gcgcgctggg ccgctgcgtc
    11701 gatgcgggga tgacggtggt cggcacggtg gtctcccgtg gccagccgct ttcggaagtt
    11761 ccggcaagcg gtcccagggt catcgtcctg ggttccgagg gtgcgctgga aggagcacgc
    11821 gcgggatcca tcctcgtgga tatgagttcc atcagtcccc tggtcgcgca gaaggtcgga
    11881 gccgcgtgcg cggccaaagg cgttg
//
LOCUS       DAOPPA010000029        11881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6212_length_11881_cov_3.860054, whole genome shotgun sequence.
ACCESSION   DAOPPA010000029 DAOPPA010000000
VERSION     DAOPPA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_11881_cov_3.860054"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKH54_01725"
     CDS             complement(<1..214)
                     /locus_tag="PKH54_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98635.1"
                     /translation="MKVCTVLFMFVIAIGLGCGSDGNGTDEGGLTDVGGQDVSAPDTN
                     TPDVETDLGQQEDANTGDTGGGIDTNT"
     gene            complement(383..2005)
                     /locus_tag="PKH54_01730"
     CDS             complement(383..2005)
                     /locus_tag="PKH54_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98636.1"
                     /translation="MKKLLSFAVACALCGFAVACGDDGNPNPGEDIYIPTDMNQGDEG
                     TDVNGQPDVIANPDALPDNAQPDEGGQPDLVTPDQGVDEGGQTDEGGTDTNLPPCEDG
                     VGSCKWYWDVCAAGCPNPQTAEGEACMAECQGQLTAEGQEDLQVFNNCIYNICGSSPD
                     EEAWYACIDENCTEAIIGCYWGCTYRTCAEMYDCFDGCPEDDPGTSDNETSMCYGDCR
                     NESTEDAQLDQNAMYNCAFDKCPICETYDTAAEEEECNVCFNDAVMGDCWDYYKNCVS
                     GGTKYQDCVDFTICFTTCQYTAQTQAAYDACVDECQAGVTPEGLRDRIMAENCSLDNC
                     PVCQVEEPTAQQDEECNTCWMEAMYGDDEGVGVCNAEWSKCAGTENETCMEYYTCAGG
                     CQDYACVADCRGNTTLAGMKDMIIASECSSTACPVCDVENPTAQQKTDCDNCWMASQK
                     GDCADEWNVCLPHGDDTCAEVFNCIQGCQDSPCAQACYGNGSFDAQDLYAAFEECLQT
                     ECASASDFSACANTAINAGGACADEYGACVPS"
     gene            2167..3417
                     /gene="pyrC"
                     /locus_tag="PKH54_01735"
     CDS             2167..3417
                     /gene="pyrC"
                     /locus_tag="PKH54_01735"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOC98637.1"
                     /translation="MADLLFSEVRLPGLDGPETFDVVVDGGLIVDVGRHFQGCAASVV
                     PGRGRLLMPGAIDMHVHFREPGATHKEDLASGSNAALLAGVTTVCDMPNNTPPITTAS
                     EFMSKAELAAKSGRCDVMLYMALTASNLEQVAMVKSHPAFAGVKVFLGATTGNMICDI
                     DAVARAAAVLDCLFVFHAESDVVLRQVAARLGEGTSAGDHLALRPVIAATTAVRQIAS
                     TWRPGMRFHICHVSCREEVEIISSSGGITCEAAPHHMGLSAADTGRLGNLAKMNPPLR
                     DSEDAESVLQAVCSGAIDCVATDHAPHTLAEKAVPYPKAPAGVPGVDTLVPFVMSLVR
                     SGRMTTRRAAAVTSGNPARILGLSDRGAIAPGLRADLYLTDPSREWVVSDREIRSKCG
                     WSPFSGRTLSSRPDVVCFKGRLVE"
     gene            complement(3445..4323)
                     /locus_tag="PKH54_01740"
     CDS             complement(3445..4323)
                     /locus_tag="PKH54_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98638.1"
                     /translation="MTHDRKTLLVVAVLALVLASGCSKARKDSVSYCNQGIIEYEAGN
                     LKEAIGLFDAAISVYPRNQVAHFQLGSILMNDKKDYAGARRELTAALELNPKDVETVY
                     LLGRLELAEGNLDPALARFQEALKIDPVHPGSLYYTGSIFQKKGKLDDADDYYRKALQ
                     ANPKYARAYNSLGMMYYDTENYQAAAAVLAEGVRLNHDDPDLHHNLGLAYLTLGKVDP
                     AVEELTASIELDPDNATAAFNLANALIRQQRFKQATFYLQRFIVAPELQESELLEPAK
                     LTFNALKQAMAQGIGL"
     gene            complement(4351..6096)
                     /locus_tag="PKH54_01745"
     CDS             complement(4351..6096)
                     /locus_tag="PKH54_01745"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOC98639.1"
                     /translation="MDGKTPTIRIQIFEGDSLVREEVFTSTPIRIGKAGQAHLRLTDP
                     AVSRQHAVIDYLENGEVRITDSQSTAGTFVQGRKVTQSALQPGNEIVVGTTRLVIDYV
                     AEASAIKAPETFQSSVSQSDLKTGRFGVEVSLNWEDYPLAHGFFPVGSTVKVGEGTKD
                     DFFVPEAKTGFESFVFLKPDDKFFLMNVAGDRIDADFLVDDKIIRLPEMKRRGLVMGD
                     EWVRIDHRTAARVRYGDLVFVVGLSSQPRKVMGSSWKRFSMREHMYLLLSLMIHLALM
                     LLIALVPEEQLKATRDPYDRETKALRKIQVAMLERKVEEEKKKEEEEIKKKFEEAKED
                     DGMVDRLSRAEPTDKQTSKIVVADTETDRNVANQALTQVMGEQDARFGRLLDMAGSGL
                     GGGTMGIRVIGDNGIDADLASSLGAFGGTMGGGAGGFQGTGAWGGGGEYAPADIRGIS
                     GLSKKDAEGASSKIKFKKGDTQLQVFAGDMKVGALDKEMVRRYVQSKMNQIRWCYQQE
                     AQKNPELAGNVDMTWVILPTGYTAGVKVRDTTMRNAAVERCIMERIATWRFPEPKGGA
                     AVQVSSYPFIFRLTK"
     gene            complement(6112..6375)
                     /locus_tag="PKH54_01750"
     CDS             complement(6112..6375)
                     /locus_tag="PKH54_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98640.1"
                     /translation="MNRLLLWVAAAMVFQAGVFVSGAVVAQNKDAKPTAVEEMSFEGD
                     LVEAQFLRPDQGVTEALLRKKRSSLIKLRTDFVDEILKSAEDL"
     gene            complement(6457..>9018)
                     /locus_tag="PKH54_01755"
     CDS             complement(6457..>9018)
                     /locus_tag="PKH54_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98641.1"
                     /translation="IAASDTYLKMAKPDSPNVVETRFVRAMAYYKYNHFEEAAAGFTD
                     IISRDPDHVNAPDAARLLLSSLALMRDIPRLNAAVDTIQVQPALMTGEVPEIIRKINE
                     RKDFNACFEFEKKGQYSRAAECFLAYVAKFQSTPLKDSALINAANNFFKARRVEDSLK
                     VNEQLVNEFPNSDLGKRALYNIADTYRRLAVYSEAARIYETFVDNYPNHELTEEGLRF
                     ATMLRTGLGDNDQAIKDLRKYIKLFPKSEHIAGIAIEIAAILSKQGKHSQAIAEYNAY
                     IKKYGQSGGPDRLLLANLKIAQAYGKMGGYKNYVASQQAYDKTIAVYDTMSDEVKQKV
                     SPIGLASVAEAMFVKGEGVLDKMRAVKLSGNMNVVTDQIASKLALAEEAMKIFNTVEA
                     FGQPNWTIAAFSRKGFGFQELAETIENTPAPRGLSETQKQFYQEGLGQRAIPIWEKAK
                     DSYRECIQLAQELKWYNKYSEEAEDALMKLDPEFRTLADLRPRPEIYSVNNGRMTFLA
                     EKEGMDPPKWSDANLEQRVRQGAEAADATPEALYNMGALLKGRARLAESRSWFERAAA
                     ARADFDKAIAAIGFVLAQEGRISEAEAQFKAALKIDPANAIANAFYAARALSKRSFTE
                     AINLARDGLVSDPDSRDAYAVLGAAYLEMGLIDAGLLVARNAISLDPTDGQIQNLMAI
                     MFGRQNEVRQAVKMFSKATQDAPELYDAWMNLGLVTLAYKDLKTAGDAFDRALALKPA
                     SRDARLAKAVVLRGGNQGDAAMKILKELESEGQDPDVHFNLCLVYQENLSREEDALDE
                     CGKFLSLIPADHPKRKEVSRRIEGIRITIEALKDSPPPAPEPPATEPAVGQDTAN"
     assembly_gap    9019..9028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9029..10668)
                     /locus_tag="PKH54_01760"
     CDS             complement(<9029..10668)
                     /locus_tag="PKH54_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98642.1"
                     /translation="MMGHTGIRLLAAVAASFLAAAAGYSVTASAQVAREKSVQTFSLS
                     DDGAPGGSSVLEILKTEDQEKGAKPLGVEGIQSRPKAMADEKLEASIATLNDLIEEAD
                     DDDPSKPEYLARLGEVYWDKAENFFNKAQGDQITSRLRSAIDSKDDATIQKIEAERAD
                     LMEQRAYWQMESVNAYRRVVDNFPEYPALDETLYYLGFALVQMGKQADAYPYFARLVR
                     ESPNSQYVPDALLNIGEYYFNVGQMEDALKIYTEVENFKDSTAYGLAIYKKGWCFFNL
                     ARHEEAMNQFLVVIDYTGTEQAAKSGYGRQLLKEAQRDLVMAYSSLPDGSPDSAIRVF
                     KAISPDGYMDLAIKLAEGYASQGDFQKSTRLYKNIIAEYASRPDAWKVVQFQREIFEN
                     VYRAAVKKEVVEEARRLIGLLDKFRAVAPKDQIQPELVRVEELIRVVATTYHNEARKT
                     MEDATLEFTHHLYNEYLRLFPDAEKSFTILYNYALLLQDIGKFELAAERFSQVVAARP
                     SDPLADDAAHAAVSAYHKLLENQPNRAKSEDTTDTEPK"
     gene            complement(10735..>11881)
                     /locus_tag="PKH54_01765"
     CDS             complement(10735..>11881)
                     /locus_tag="PKH54_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98643.1"
                     /translation="ESADEISFNVGQYFYYTGNLNQALESLMRVSTAVPELYVKAMYF
                     KGVVFVRRNDARAAASTFQEVLAYLDRYDVPNGERYRVMANLATARVFYSVGQSTPAE
                     FKTAIKYFDRVPDSSEYWLDSLFEKSWAYFMDGNYARALGNLITVSSPYFEEEYYPEA
                     NVLRAVIFFMNCLYDEALQTVDPFYKEYYDISKELRRVLAKYEDPASFYQYLASLSKT
                     GGSAYSVKVKKIFNAALADRKLRRLFGYVVAINNEITRLDDMGKKSGASALADFLVPE
                     LESYRSLTIAEAGQLARERLERMSKELRELLSQALKVRFESLNAQKGILGENFRQEQL
                     AAAQAGSDGGSIKIDEEHQLWPWNGEYWKDELGSYYYPIKSVCGNQQ"
BASE COUNT         2261 a         3605 c         3421 g         2584 t
ORIGIN      
        1 tcgtgttggt gtcgatgccg ccgccggtat caccggtatt ggcatcttcc tgctgcccca
       61 ggtcggtttc cacatccggg gtgttcgtgt cgggcgccga aacatcctgg ccgccgacgt
      121 cggtcaaacc accttcatcc gtgccgttgc catctgatcc acaaccaaga ccaatggcaa
      181 tgacgaacat gaacagaacc gtgcagactt tcatgattcc cccgatatgg atttgttgac
      241 aaaccgttaa ccgaccatcg gatgttacac gatcgacagt aaaacgaaaa gtgtcgcttt
      301 cggacagtgt caccctaaaa aaaaggggcc gccgggaaac ccggcgaccc ccttttcatc
      361 ggtcaatcaa gcgaaggccg aatcagctcg gaacgcaggc gccgtattca tcggcacagg
      421 caccacccgc gttgatggcg gtgttggcgc aggcggagaa gtcggacgcg ctggcgcatt
      481 cggtctgcag gcattcttcg aacgccgcgt acaggtcctg ggcatcgaac gaaccgttgc
      541 cgtagcacgc ctgggcgcag ggggaatcct ggcagccctg gatgcagttg aacacttcgg
      601 cgcaggtgtc atcgccgtgc ggcagacaaa cgttccattc gtcggcgcag tcgcccttct
      661 gggacgccat ccagcagttg tcgcaatcgg tcttctgctg agccgtggga ttttccacgt
      721 cgcagaccgg gcacgcggtg gacgagcatt cggacgcgat gatcatgtcc ttcatgccgg
      781 ccagcgtggt gttgccgcgg caatcggcca cgcatgcgta gtcctggcaa ccaccggcgc
      841 aggtgtagta ttccatgcag gtctcgtttt cggtgccggc gcacttcgac cattcggcat
      901 tgcagacgcc aacgccttcg tcatcgccgt acatcgcttc catccagcag gtattgcact
      961 cttcgtcctg ctgggcggtg ggttcctcga cctggcagac cgggcagtta tccagcgagc
     1021 agttctcggc catgatacga tcacgcagac cttccggggt cacgccagcc tggcattcgt
     1081 caacgcaggc atcgtaggcg gcctgggtct gggcggtgta ctggcaggtc gtgaagcaga
     1141 ttgtgaagtc aacgcaatcc tggtacttgg tgccgccgga aacacaattc ttgtagtaat
     1201 cccagcaatc gcccataacc gcatcgttga agcagacatt gcattcttct tcctcggcgg
     1261 cggtgtcgta cgtttcacaa atggggcact tgtcgaaagc gcagttgtac atcgcgttct
     1321 ggtcaagctg ggcgtcctcg gtgctttcgt tgcggcagtc gccatagcac atgctggtct
     1381 cgttgtcgga cgtgcccgga tcatcctcgg ggcaaccgtc gaagcagtcg tacatctcgg
     1441 cacaggtgcg gtaggtgcag ccccagtagc agccgatgat tgcttcggtg cagttttcat
     1501 cgatgcaggc ataccaggct tcctcatcag gggaactgcc gcagatgttg taaatgcagt
     1561 tgttgaaaac ctgcaggtct tcctgtccct cggcggtcag ctggccctgg cactcggcca
     1621 tgcaggcttc gccttcggcg gtctggggat tcgggcaccc ggcggcgcag acgtcccagt
     1681 accacttgca ggaaccgacg ccatcctcgc acgggggcag gttggtgtcc gtgccgcctt
     1741 cgtcggtctg gccaccttcg tcgacgccct gatccggcgt gacgagatcg ggctgtccgc
     1801 cctcgtccgg ctgagcgttg tccggaagcg catccgggtt ggcaataacg tccggctggc
     1861 cgttgacgtc cgtaccttcg tcaccctggt tcatgtcggt ggggatgtag atgtcctcgc
     1921 cggggttggg gttaccatcg tcgccgcagg caacggcgaa gccacaaagg gcacacgcga
     1981 cggcaaaact cagcagtttc ttcattctgg ctctccttcg attgaccaaa aaaatccacg
     2041 tcaacagggc agtcccccga agaccaaaca ttccgaaccg accaattttc gtgattttgc
     2101 agcaatttgt caacgattca atgctatgat cggatacgtt tttttttccc gaaggcggtg
     2161 gttgccgtgg cggatttgct gttttcagaa gtaaggcttc cggggctgga cggccccgaa
     2221 acatttgacg ttgtcgttga tggcggcctg attgttgacg tcggcaggca ctttcagggg
     2281 tgtgccgctt ccgttgttcc cggtcggggg cgacttctga tgcccggggc catcgacatg
     2341 cacgttcact tccgcgagcc cggcgccact cataaggagg acctcgcatc cgggtcgaac
     2401 gccgcgttgc tcgcgggagt caccacggtg tgcgacatgc cgaacaacac gccgccgatc
     2461 acgacggctt ccgagttcat gtctaaggcc gaactggcgg cgaaaagcgg gcgttgcgac
     2521 gttatgcttt acatggcgct gaccgcgtcg aatcttgagc aggtggcaat ggtgaagtcc
     2581 catccggcat tcgccggggt aaaggtcttc cttggcgcca cgacgggaaa catgatctgt
     2641 gacatcgacg cggtcgcgag ggcggcggcg gttcttgact gcctgttcgt gtttcacgcg
     2701 gagtcggatg tcgttctcag gcaggtggcc gcccggcttg gcgagggaac ctcggccggc
     2761 gaccatcttg cccttcggcc agtgattgcc gccacgacgg cagtacggca gatcgcctcg
     2821 acctggcgtc ccggaatgcg tttccacatc tgccacgtat cctgtcgcga ggaagtcgaa
     2881 atcatttcgt catccggggg aattacctgc gaggcggcgc cccaccacat ggggctttca
     2941 gcggcggaca cgggaaggct gggaaatctt gccaagatga acccgccgct tcgcgacagc
     3001 gaggacgcgg aatcggtgct gcaggcggta tgttcgggcg ccattgactg cgttgcaacc
     3061 gatcatgcgc cgcacacgct tgcggaaaag gcggttccct acccgaaggc ccctgccggt
     3121 gttcccggtg tggatacgct ggttccattt gtcatgtccc tggttcgaag cggacgcatg
     3181 acgaccaggc gcgccgccgc ggtgacttca gggaaccccg cgcggatact cggactttca
     3241 gaccgcggcg ccattgcgcc gggactgcgg gcagaccttt acctgaccga tccgtcccgg
     3301 gaatgggttg tttccgacag ggaaataaga tcgaaatgcg gttggagccc cttttccggc
     3361 cggactcttt cttccaggcc ggacgtcgtg tgcttcaagg gaaggctggt ggaatagctg
     3421 gggccgcccg gctgacaaat gggcttacag cccgataccc tgggccatgg cctgcttcag
     3481 cgcgttgaag gtaagcttcg ccggttcgag aagctcggat tcctgaagct cgggggcgac
     3541 gatgaacctc tgaaggtaga acgtggcctg cttgaaccgc tgctggcgga tcagggcgtt
     3601 ggccaggttg aacgcggcgg tggcgttgtc cggatcgagc tcgatgctcg cggtgagttc
     3661 ctcgaccgcg ggatccacct ttccaagggt caggtatgca agtcccaggt tgtggtgaag
     3721 atccgggtcg tcatggttca gcctgacacc ctcggccaga accgcggcgg ccgcctgata
     3781 gttctccgta tcgtaataca tcatccccag cgagttatag gcgcgggcgt acttcggatt
     3841 tgcctgaaga gccttcctgt agtagtcgtc ggcatcgtca agcttgccct tcttctggaa
     3901 gattgagcct gtgtaataaa ggcttcccgg atgcacggga tcaatcttca acgcttcctg
     3961 gaagcgggcc agcgcgggat caaggttacc ttccgccaac tcaagcctgc caagaagata
     4021 gaccgtctca acgtctttcg gattcagctc gagcgcggca gtcagctcgc gccgggcgcc
     4081 cgcgtaatcc ttcttgtcgt tcatcaggat tgagccgagc tggaaatggg ccacctggtt
     4141 cctgggatag accgaaatcg ccgcatcgaa aaggccgatt gcttccttca aattaccggc
     4201 ctcgtattcg attatcccct gattgcagta tgaaaccgag tcctttcggg ccttcgagca
     4261 cccggacgcc agcaccagcg caagaaccgc gacgacaaga agcgtctttc tgtcatgagt
     4321 catggctgca atcctcatca ggagaataca ttacttggtc aggcggaaaa tgaagggata
     4381 cgacgaaacc tgaaccgcgg cgccgccctt cggttccggg aaccgccagg tggcgatgcg
     4441 ttccattatg catctttcaa ctgcggcatt gcgcatggtg gtgtcccgaa ccttgacgcc
     4501 cgcggtgtat ccggtcggaa ggatgaccca cgtcatgtca acgttgcccg ccagttccgg
     4561 gttcttctgg gcttcctgct ggtagcacca cctaatctgg ttcatcttcg actggacgta
     4621 tcgccgcacc atttccttgt cgagcgcgcc gaccttcatg tcgccggcaa atacctgaag
     4681 ctgggtgtcg cccttcttaa acttgatctt cgagcttgca ccctccgcat ccttcttcga
     4741 caggccggat atcccgcgga tatcggcagg cgcgtattcg ccaccgccac cccaggcccc
     4801 ggtgccctgg aagccgccgg cgccgccgcc catggttccg ccgaacgcgc caagcgaact
     4861 ggccagatcg gcgtcgatgc cgttatcgcc gatcacgcgg atgcccatgg taccgccgcc
     4921 gagtcccgaa ccggccatgt caagcaggcg gccgaaccgc gcgtcctgtt cccccatgac
     4981 ctgggtcaac gcctgattgg cgacgttccg gtcggtctcg gtgtccgcca cgacgatctt
     5041 cgaggtctgc ttgtctgtcg gctccgcgcg gctgagacga tcaaccatgc cgtcgtcttc
     5101 cttggcctcc tcgaacttct tcttaatctc ttcctcttcc ttcttcttct cttcctcgac
     5161 cttgcgttcc agcatcgcga cctgaatctt ccgcaacgcc ttggtctccc ggtcgtacgg
     5221 atccctggtc gccttcaact gctcttcggg aacaagcgct atcagaagca tcaacgccag
     5281 gtgaatcatc agggaaagca gcaggtacat gtgctcccgc atgctgaaac gcttccagct
     5341 tgagcccatt accttacgcg gctggctcga aagcccaacc acgaaaacaa gatccccgta
     5401 tcggacgcgt gcggcggtgc gatggtcaat ccgaacccac tcgtcgccca ttacaaggcc
     5461 gcgacgcttc atctcgggta aacggataat cttgtcgtcg acaaggaaat cggcatcgat
     5521 acgatcgccg gcaacgttca tcaggaagaa tttgtcatcg ggcttcagaa atacgaacga
     5581 ctcgaatcca gtcttggctt ccggaacgaa gaagtcgtcc tttgttccct cgccgacctt
     5641 gaccgtgctc cccacgggga agaagccatg cgccagcggg tagtcttccc aattgaggct
     5701 gacctcgaca ccgaaccgcc cggtcttgag atccgactgt gacaccgaag actggaacgt
     5761 ctcgggagcc ttgatcgcgg aggcctcggc cacatagtca atcacgagcc tggtcgttcc
     5821 gacgacaatc tcgtttccgg gctgcagggc gctttgcgtc accttccgcc cctggacgaa
     5881 agtaccggcc gtgctctgcg agtccgtaat ccgaacctcg ccgttttcga gatagtcaat
     5941 tacggcatgc tgacgactca ccgcgggatc ggtgagtcgg aggtgggcct gacccgcctt
     6001 cccgattcgt atcggcgtcg atgtgaagac ttcttcgcga acgaggctgt cgccctcgaa
     6061 gatctgaatt cgtatggtcg gcgtctttcc gtccatggca cccccggatg cttaaaggtc
     6121 ctcggcggac ttgagtattt cgtcaacgaa gtcggttcgt aacttaatca gactggatct
     6181 cttcttccga agaagcgcct cggtaacgcc ctggtcgggt cgcaggaact gcgcctcgac
     6241 aaggtcgccc tcgaaactca tctcctcaac ggcggtcggc ttggcatcct tgttctgggc
     6301 cacgacggca cccgaaacaa aaacacccgc ctgaaaaacc attgcagcgg cgacccaaag
     6361 cagcagcctg ttcatgaagc acctcactgg cgcaaatcgt aatcccgcga acccaaaaac
     6421 acaacgggag cctttgacag cacccgtgga taaccgctaa ttcgcagtat cctgcccaac
     6481 ggccggttca gtggccggcg gctccggggc cggcggcggt gaatccttca acgcctcgat
     6541 ggtgatccgg atgccctcaa tcctgcgcga aacttccttc cgcttggggt gatccgcggg
     6601 aataagggaa aggaacttac cacattcatc aagcgcgtcc tcctcccgcg agaggttttc
     6661 ctgatacaca aggcacagat tgaagtgaac atcgggatcc tggccttcgg attccagttc
     6721 cttcaggatc ttcatcgcgg cgtcaccctg gttgccgcca cgcagcacga cagccttggc
     6781 gagtcgggca tccctgctgg caggcttcag ggccagagca cgatcaaatg cgtcgccggc
     6841 ggtcttcaga tccttgtatg cgagggtcac caggcccagg ttcatccacg cgtcgtaaag
     6901 ttcgggcgca tcctgggtgg ccttggagaa catcttgacg gcctggcgga cttcattctg
     6961 cctgccaaac atgatagcca tcaggttctg gatctggccg tccgtcggat ccagcgaaat
     7021 cgcgtttctc gcaaccagaa ggccggcatc gataagcccc atctcaaggt atgccgcgcc
     7081 aagaaccgcg tacgcgtcac ggctgtcggg atcggaaacc agtccgtcgc gcgccaggtt
     7141 gatggcctcg gtgaagctgc gcttcgacag tgcgcgcgcc gcatagaacg cgttggctat
     7201 ggcgtttgcc gggtcaattt tcaacgcggc cttgaactgg gcctcggcct cggagatccg
     7261 cccctcctgg gccagcacga acccgattgc cgcaatcgcc ttgtcgaaat cggcccttgc
     7321 cgccgccgcc ctctcgaacc agcttcggga ttcggcaagc ctggcccttc ccttcagcag
     7381 cgcacccatg ttgtaaaggg cttcaggcgt tgcgtcggct gcctcggcgc cctggcggac
     7441 cctttgctcg aggttggcat ccgaccactt cggcggatcc attccctcct tctcggcgag
     7501 gaaggtcatc cgtccattat tgacagaata gatctcggga cggggacgca ggtcggcaag
     7561 cgttctgaac tcgggatcca gcttcatcaa tgcgtcctcg gcctcttccg agtacttgtt
     7621 gtaccacttg agttcctggg cgagctggat gcactcgcga tacgaatcct tcgccttctc
     7681 ccagatcggg atggcccgct ggccaagacc ttcctgatag aactgcttct gggtctcgct
     7741 caggcctctt ggcgccggcg tgttctcaat cgtttcggcc agttcctgga agccgaagcc
     7801 cttccttgaa aacgccgcaa tcgtccagtt gggctgaccg aaggcttcaa ccgtgttgaa
     7861 gatcttcatc gcctcctcgg caagggccag cttcgatgca atctgatcgg taaccacgtt
     7921 catgttgccc gagagcttga ctgcccgcat cttgtcaagc acgccctcgc ccttgacgaa
     7981 catcgcctcg gcgaccgacg cgaggccgat tggagaaacc ttctgcttca cttcgtccga
     8041 catcgtgtca tacacggcga tggtcttgtc gtacgcctgc tgggaagcca cgtaattctt
     8101 gtatccaccc atcttcccgt aggcctgggc gatcttcagg ttcgccagca gcaggcggtc
     8161 gggaccgccg ctctgtccat atttcttgat gtatgcgttg tactcggcga tggcctggga
     8221 atgcttgccc tgcttggaaa ggattgcggc gatctcgatt gcgatgcccg cgatgtgttc
     8281 cgacttcggg aacaacttta tgtacttgcg caaatccttg attgcctggt cgttgtctcc
     8341 cagaccggtt cgcagcatcg tcgcgaaccg caacccttcc tcggtaagtt cgtgattcgg
     8401 gtaattgtcg acaaaagtct cgtaaatgcg cgcggcctcg gaatagacgg ccaggcggcg
     8461 atacgtgtct gcaatgttgt aaagagcacg cttgccgaga tcggaattcg ggaactcgtt
     8521 gacgagttgc tcgttgacct tcagggaatc ctcgacccgg cgggccttga agaaattgtt
     8581 tgcggcattg ataagggccg aatccttcaa cggcgttgac tggaacttgg ccacgtacgc
     8641 aaggaagcac tcggcggccc gactgtactg gcccttcttc tcgaattcga agcaggcatt
     8701 gaagtccttc cgctcgttaa tcttcctgat gatctcgggc acctcgccgg tcatcagggc
     8761 cggctgaacc tgaatcgtgt caacggccgc gttcagccgc ggaatatcgc gcatcagggc
     8821 cagggacgaa agcaggaggc gggcggcgtc tggcgcgttc acgtggtctg gatccctcga
     8881 aatgatgtcg gtgaacccgg cggcggcctc ctcgaagtgg ttgtacttgt aatatgccat
     8941 tgccctgacg aacctggtct cgaccacgtt gggtgaatcc ggcttggcca ttttcagata
     9001 ggtgtcggac gcggcgatnn nnnnnnnntc cttcggttcg gtgtcggtcg tatcttcgga
     9061 cttggcccgg ttaggctgat tctcgagcag tttgtgatac gccgaaaccg cggcgtgcgc
     9121 ggcgtcgtcc gccagggggt cgctgggacg ggcggccacg acctggctga acctttccgc
     9181 ggccagctcg aactttccga tatcctggag cagcaatgcg tagttataga gaatcgtgaa
     9241 tgacttctcg gcatccggga acagccgcag atattcatta tatagatgat gcgtgaattc
     9301 cagggtcgca tcctccatcg tcttgcgggc ctcgttgtgg taggtggtcg ccacgacgcg
     9361 gatcagctcc tcgacgcgaa ccagttccgg ctgaatctga tccttcggag ccaccgcgcg
     9421 gaacttgtcc agcaacccga taagacggcg cgcctcctcg accacttcct tcttgacggc
     9481 ggcgcgatag acgttctcga aaatctcacg ctggaactgg acgaccttcc atgcatcggg
     9541 cctggaggcg tattccgcaa tgatgttctt gtacagcctg gtcgacttct ggaaatcacc
     9601 ctggctggcg tagccctcgg ccagtttgat ggccagatcc atgtaaccgt ccggggagat
     9661 cgccttgaag accctgatcg cgctgtcggg ggagccgtcc ggcaggcttg agtacgccat
     9721 gaccaggtcg cgctgggctt ccttgagcaa ctggcggccg taaccgctct tggcggcctg
     9781 ctcggtgccg gtgtaatcga tgacgaccag gaactggttc atggcctctt catgacgcgc
     9841 caggttgaag aagcaccagc cctttttgta gattgccagt ccataggcgg tcgaatcctt
     9901 gaaattctcg acctcggtgt agatcttcag ggcatcttcc atctggccga cattgaagta
     9961 gtactcgccg atgttcagaa gggcgtcagg gacgtactga ctgtttggcg attcacggac
    10021 cagacgcgcg aagtagggat aggcatctgc ctgcttgccc atctggacaa gcgcgaaacc
    10081 caggtaatac agagtctcgt caagtgcggg atactcgggg aagttatcaa cgacacgcct
    10141 gtaggcgtta accgactcca tctgccagta tgcgcgctgc tccatgaggt cggccctttc
    10201 ggcctcgatc ttctggatgg tggcgtcgtc cttcgagtcg attgccgaac gaagcctgga
    10261 agtaatctgg tcgccctgcg ccttgttgaa gaagttttcg gccttgtccc aatagacctc
    10321 gccaagcctt gcaaggtatt ccggcttgct cgggtcgtcg tcgtcggcct cctcgatcag
    10381 gtcattcagc gtggcaatgg acgcttccag cttttcatcg gccatggcct tgggtcggct
    10441 ctgaatgccc tcgacgccaa ggggcttggc ccccttttcc tgatcctcgg tcttcaggat
    10501 ctccaggacg gaggatccgc caggcgcgcc atcatcggaa agggaaaacg tctgaaccga
    10561 cttctccctg gctacctgcg cgctggccgt cacggaatat ccggccgcgg cggccaggaa
    10621 ggaagcggcc acagccgcca gaagcctgat gcctgtatga cccatcatac aaatcctccg
    10681 tctgaaccgg cgcagcggcc gatccaaatc cgagctgtca gggcgacgcc gaggtcactg
    10741 ctgattaccg caaaccgact taatcgggta ataatacgaa ccaagttcat ccttccagta
    10801 ttcgccgttc cacggccaga gctggtgctc ttcatcgatc ttgatgcttc cgccgtcact
    10861 gccggcctgc gccgcggcaa gctgctcctg cctgaagttt tcgcccagga tgcccttctg
    10921 ggcgttgagc gactcaaaac gaaccttcag ggcctgggaa aggagctcgc gaagctcctt
    10981 gctcatgcgc tccagacgtt cacgcgcaag ctgtccggcc tcggcaatcg tcaacgacct
    11041 gtaagactcc agttcgggaa ccaggaagtc cgcaagcgcc gaagcgccgg acttcttgcc
    11101 catgtcgtcc aaccttgtga tttcattatt gattgcaacg acgtatccga agaggcgccg
    11161 aagcttgcga tcggccagcg ccgcgttgaa aatcttcttg accttgacag aataggcact
    11221 tccaccggtc ttcgaaagcg acgccaggta ttgatagaaa gatgccggat cctcgtactt
    11281 ggcgaggacg cgccgcagtt ccttgctgat gtcgtagtat tccttataga acggatcgac
    11341 cgtctggagc gcctcgtcat aaagacagtt catgaagaag atgaccgccc gcaggacgtt
    11401 cgcctccggg tagtactcct cctcgaagta gggagaactg acggtaatca ggttgccgag
    11461 cgcccttgcg tagtttccat ccatgaaata ggcccaggac ttctcgaaaa gactgtccag
    11521 ccagtactcg gaactgtcgg gaacgcgatc gaaatacttg atggccgtct tgaattcggc
    11581 cggcgttgac tggccgacgg aatagaaaac acgtgccgtg gccaggttcg ccatcacccg
    11641 gtacctttca ccgttcggga cgtcatagcg atccaggtat gcaagaacct cctggaaggt
    11701 cgaggccgcc gcgcgcgcgt cgttgcgccg gacgaaaacg acgcccttga agtacatcgc
    11761 cttcacgtaa agctccggca cggcggtgct cacccgcata agactttcaa gcgcctggtt
    11821 gagatttccg gtgtaataga agtactggcc aacgttgaag ctgatttcat cggccgactc
    11881 g
//
LOCUS       DAOPPA010000030        11847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6234_length_11847_cov_3.983039, whole genome shotgun sequence.
ACCESSION   DAOPPA010000030 DAOPPA010000000
VERSION     DAOPPA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_11847_cov_3.983039"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKH54_01770"
     CDS             complement(<1..283)
                     /locus_tag="PKH54_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98644.1"
                     /translation="MKWQGIAGLGVSVAFHIAVIVSLALLVTVNPSDSSRKVPIGLKV
                     VDKKAAEKEAAAMGQAESGPAVAGSDRDAGGRRKARLAATAGPVVKVDSQ"
     gene            complement(319..1113)
                     /locus_tag="PKH54_01775"
     CDS             complement(319..1113)
                     /locus_tag="PKH54_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98645.1"
                     /translation="MGFDHLAIHRIFIAVALVLTQSGCASVWHLEDVVTRVARVESDI
                     AEMKKLQADDQQRLDRLHKDMVEAEETLRRSGAHLGDDMDAMKLEAARLKSADEELRF
                     SLSRTASEVGLIKRALSERLGVQTLDIPEDVLKNPEKLLAAAQDAFDSSDDRRAIELC
                     GIAIARYPDGVTAARATNLLGEIAFRAQNWATAVREFQRVHNYSKTLKEINGNQALLR
                     IGEALEKQGSCAKAIEIYQFLVDQKSRAAEEKTAETRIKKLKKTCR"
     gene            complement(1160..1942)
                     /gene="pal"
                     /locus_tag="PKH54_01780"
     CDS             complement(1160..1942)
                     /gene="pal"
                     /locus_tag="PKH54_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOC98646.1"
                     /translation="MKLARVLTIVFAITSLLTLVACGPKYPNCDSDKQCKEKGEYCYD
                     KKCVKCIDDSHCAQMGPCGKCSANHECKLPLGEIGDCCSLDGDCKSRHCLVAGGAETG
                     KCVQCKENPDCGTGMKCVNNSCVPDVECSSDTDCGQGRKCDGGKCVVDLCKMKPAYFD
                     FDEYELRTDSREALDKSLSCMKERGIENVSIEGHCDDRGSDEYNLALGNRRAAAAKKY
                     LKKMGVKDKSLSTISYGEERPTCMDATESCWSRNRRAEIVEK"
     gene            2216..3208
                     /gene="ybgF"
                     /locus_tag="PKH54_01785"
     CDS             2216..3208
                     /gene="ybgF"
                     /locus_tag="PKH54_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOC98647.1"
                     /translation="MKFAPAVIVCLLLSAMPGCAGNIDKMTVRFDEMDMRVQTLSRTN
                     NALRGRIDELENRILLLQDEVETQRLAGMRGGSQPYVTPSYSAPAAPASSLPVMKLEP
                     GDAAPEPVDFVAGSDTSEPYPTDYAFEDYDRAVQAAEPSAEPIDGGSLDIADSYQGID
                     DMGRVVPSKSKGSSKVAKSTARTEKKAPPPPPARKEKPADVAKKAAAMALEQYKAAYS
                     LYGEGRLDDAREAFNQFVIDNPRHAYADNARYWVGECWYDRREYEKARVEFMRVVTEY
                     PDGNKVPDAMVKVGLSDQNLGRMSDARRMYDAVILTYPDSEAAAVATRLIGKMQ"
     gene            3268..4311
                     /locus_tag="PKH54_01790"
     CDS             3268..4311
                     /locus_tag="PKH54_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOC98648.1"
                     /translation="MNAGFLDRVTRAILVVSVGLLSAIRVSGAVAQEIQVSGSIDDVN
                     VYVAEGGDSLWDIADRFFSDPWYWPTLWSFNPHITNPNWIFPGDRVYLVPPKPVKDDK
                     GGYAMSESRFDPGVRDELALGRRVGYMSEDEVKASGVISHSREEKEMLGERDDIYIKF
                     DTARRINRGDLVLIYRQGKRVKHPVTKKKLGFEIKYLGMAKVTDTEKDMNKAVIMKSF
                     EEIYRNDKVMAFAPVQKMVPPVSNSKEVVGSIIASIEGVRFLGEYHYVMIDKGSKDEV
                     MAGNRFMVVTTGDGVDRYNKKIKKKARKEFPEEKYGELLVIEARDSSSLCIVTYANRE
                     FEVGAKVLMPEGY"
     gene            4435..5367
                     /gene="trxB"
                     /locus_tag="PKH54_01795"
     CDS             4435..5367
                     /gene="trxB"
                     /locus_tag="PKH54_01795"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOC98649.1"
                     /translation="MQRKVVIIGSGPAGLTAAIYASRAGLSPLVLEGPAPGGQLLTTT
                     EVENFPGFENGVSGPELMDAMRRQATRFGAECRFSSATAVDFASRPFMVELDGSETVQ
                     ADSVIIATGATARYLGLPSETALRGHGVSACAVCDGFFFRGKDVAVIGGGDTALEEAC
                     YLTRFANRVFLVHRRDQLRASKAMQQRIMANPKVSFVWNSVVDEVISDDEGKSVAGLL
                     LRDVKSGSSSVLAVQGMFLGIGHAPATEIFKGQIDLDDQGYIRLSHHTSTSIEGVFAC
                     GDVADSRYRQATTAAGSGAAAAIDCERWLADQGE"
     gene            5394..6023
                     /gene="nth"
                     /locus_tag="PKH54_01800"
     CDS             5394..6023
                     /gene="nth"
                     /locus_tag="PKH54_01800"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOC98650.1"
                     /translation="MDGVRAAIADVPGPAVSMNGAITSSPFKVLVATLISLRTRDQVT
                     GPASERLFALADSPEAMIALGAPAVSAAIFPANFHPTKAERIVEVSRLLIERHAGQVP
                     ADMDALLALPGVGPKTANLVLAEGFGIDAICVDTHVHRIPNRVGLIDTRDPVQTEEVL
                     RSRLPLKYWKEINRILVPFGQSVCTPVSPKCSICPLIEGCARRFKGPSR"
     gene            6134..7453
                     /locus_tag="PKH54_01805"
     CDS             6134..7453
                     /locus_tag="PKH54_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOC98651.1"
                     /translation="MAGHPRKVVIVGAAGRDFHNFNCLYRDNDAFRVVAFTAAQIPDI
                     AGRKYPAVLAGNLYPDGIPIVEECELESLIREHQIDDVVFSYSDVSHQTVMEIGERAI
                     AAGANYVLAGSRDTELVSKKPVIAVCAIRTGCGKSQTSRYITDILHKMGLKVAVIRHP
                     MPYGDLAVQAVQRFASYDDLAKHKCTIEEREEYEPHIEAGSIVYAGVDYHAILKAAEA
                     EADVILWDGGNNDMPFYKPNLFVTVLDPLRPGHEVGYYPGMANLLMADVLVINKIDSA
                     TAEDLEVVKASVAKWNPKAPIVEAESVVSLDEQVELKGTKVLVVEDGPTLTHGGMKIG
                     AGWVVAKRGGAEIVEPLKYAVGSIKATYEKYPHCAGILPAMGYSESQLADLNATVNKI
                     PADLIIVGTPIDLRKLLKDDRKMVRVRYDLKPVRGPELADFVKKVVA"
     gene            7478..8044
                     /locus_tag="PKH54_01810"
     CDS             7478..8044
                     /locus_tag="PKH54_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98652.1"
                     /translation="MTSIDAIGKLESRFDKLVKPLDLFMLIEHVRRLDPMAFARHFKG
                     YRPQMLGRKRVVTALAFEIFTRKNEVIGDIITLLWNQTRRDVYDAMHELVSTISEDVE
                     SITEIPDERAHAFIDVLLEEFDRDEILICVRMNDVRFNEPVIVARLENGEPAPAEAAA
                     PEAANPAVVEATAEPVAIDSSNQAPNPE"
     gene            8186..8527
                     /gene="hxsA4"
                     /locus_tag="PKH54_01815"
     CDS             8186..8527
                     /gene="hxsA4"
                     /locus_tag="PKH54_01815"
                     /inference="COORDINATES: protein motif:HMM:NF038313.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser repeat protein HxsA4"
                     /protein_id="HOC98653.1"
                     /translation="MASKRVPTFKKRIQDFLSSEEGKITRGQAAAIGSFMLMAGTSGQ
                     ALAAGDADQAAAASDFGTQTHCSHASHGSHGSHGSHGSHASHGSHGSHGSHGSHGSHG
                     SHGSHGSHGSW"
     gene            8840..11146
                     /gene="topA"
                     /locus_tag="PKH54_01820"
     CDS             8840..11146
                     /gene="topA"
                     /locus_tag="PKH54_01820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOC98654.1"
                     /translation="MGKPLIIVESPAKATTIKKYLSGKYEVEASAGHIKDLPQSSLGI
                     DIQKGFAPKLEVVRGKKKIVDRLKKAASTADEVFIATDPDREGEAIAWHIAEELKKVV
                     PSARRVLFHEITRDAVRKALEHPRDLDVLMYESQLARRTLDRLVGYQISPILWKKVQG
                     GLSAGRVQSVAVRLVVEREREIEAFDSKEYWLVHAVVHKDEAQSKPFKALLRKIDGRK
                     AELSGKAVTFELVERLREASFRIASLVRKDRPRAPSPPYITSSLQQDASRLLHFSPSH
                     TMSVAQRLYEGVDLGDEGRVGLITYMRTDSVRLSADSVTEARSVIASQFGPDYVPARQ
                     VEYRNRRSAQDAHEAIRPTSAERTPASLKGVLERDEQKLYALIYNRFLACQMRPAIFD
                     QTTVDIEAAGAEFRAAGSVMKFDGWLAVWRLDKSARLEDVETSEPEDEGDGGQRLPSS
                     LVEGDALLLDDLLTEQKFTEPPPRFTEATLVRELEERGIGRPSTYASIVSTIQDKSYV
                     YKHEGRLRPSELGRIVNDLLSGHFRGVVDYDFTARMEQDLDLIEEGRLDRLELLEKFY
                     LEFSRTLEQAQGEMRSVKVDAVPSGVRCPECSKELLIRLGKNGAFLGCEGYPECRVTR
                     EFDRDVKGHVVVRESPDVGTCPTCQAPLQIRSGKFGKFVACSRYPDCRFTMPYSRGER
                     CPVDGCAGRLVERQSKKGKKFFSCSAYPGCRFITSYEPLEETCPECGAPVLFVRSFRG
                     TRTVMCQREGCGFTRRVLARKAAGSGQG"
     gene            11146..11709
                     /locus_tag="PKH54_01825"
     CDS             11146..11709
                     /locus_tag="PKH54_01825"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOC98655.1"
                     /translation="MENPRQRSGDSGMTSLGDGTMVSKGSDRVSAYGAIDELDCFAGL
                     ARVEVLESLPPGPDRDLAVRSLETVQNACWLLAASLAAPGRECPEAPQIQASIEQLAV
                     EMERAAGPVSGFVIPGSSRSEALLHVARAVCRRAEREVVELFGVLGDGAAIGWELAVL
                     NRMSSLLFAASRLALKARGIPVSYRGR"
BASE COUNT         2335 a         3397 c         3687 g         2428 t
ORIGIN      
        1 cctggctatc gaccttgacc acaggcccgg ctgtcgccgc cagcctggcc tttcgacggc
       61 cgcccgcgtc ccggtcgctt ccggccaccg ccggcccgct ttcagcctga cccattgccg
      121 cagcttcctt ttcggcggcc ttcttatcga caaccttcag gccgatcggc accttcctgg
      181 acgaatcgga tgggttcacg gttaccagca gggcaaggga cactatgact gcgatgtgga
      241 atgcgacgct gacgccaagg ccggcaatgc cctgccactt catgatgcgg ttccgtcggc
      301 aaggaccggc agctttgatt acctgcaggt cttcttaagc ttcttgatgc gggtttcggc
      361 ggttttttcc tctgccgccc ggctcttctg gtcaaccagg aattggtaga tctcgattgc
      421 cttggcgcag cttccctgct tttccagtgc ttcgccgatt cgcagcaggg cctggttgcc
      481 gttaatttcc ttcagggttt tcgagtagtt gtgaacacgc tggaattcgc gcaccgcggt
      541 tgcccagttc tgagccctga aagcgatttc cccaagcagg ttcgtcgccc ttgccgccgt
      601 caccccgtcg ggataccggg caatggcgat gccgcaaagc tcgattgcgc gtctgtcgtc
      661 agatgaatcg aacgcgtcct gagcggccgc aaggagcttt tccgggttct tcagcacgtc
      721 ctcggggatg tccagcgtct ggacgccgag cctttccgat agtgcccgct taatcaggcc
      781 aacctcggac gccgtccggg aaaggctgaa acgaagctcc tcgtccgcgc tcttcaacct
      841 cgcggcctcg agcttcatcg catccatgtc gtcgccaagg tgagcgccgc ttctgcgaag
      901 ggtctcctcg gcctcgacca tgtccttgtg cagccgatcc agtctctgct ggtcgtcggc
      961 ctgcaatttc ttcatttcgg cgatatcgct ttcaaccctg gccacacgcg tcaccacgtc
     1021 ttccaggtgc cagaccgacg cgcaacccga ctgggtcaga accaatgcga ccgcaatgaa
     1081 gattctatga atggcaagat gatcgaagcc catgttgaag cttccaggga aatggcccgg
     1141 gctggccgga caagtcaggt cacttctcga cgatttcggc gcggcggttc ctgctccagc
     1201 agctttccgt cgcatccatg cacgtcgggc gctcctcgcc gtagctgatg gtcgagaggc
     1261 tcttgtcctt gacgcccatc ttcttcaggt acttcttcgc ggctgccgcg cgcctgttgc
     1321 caagggccag gttgtattcg tcggaaccgc gatcgtcgca gtgaccttcg atggaaacgt
     1381 tttcgatgcc gcgttccttc atgcaggaca ggctcttgtc cagggcctcg cgcgaatcgg
     1441 tgcgcagttc gtattcgtcg aaatcgaagt aggccggctt catcttgcag agatcgacga
     1501 cgcacttgcc gccgtcgcac ttgcgaccct ggccgcagtc cgtgtcggac gaacattcga
     1561 cgtccgggac gcaactgttg ttgacgcact tcatgccggt gccgcagtcc gggttttcct
     1621 tgcactggac gcactttccg gtttcggcgc cgccggcaac caggcagtgc ctgctcttgc
     1681 agtcgccgtc gagcgagcag cagtcgccga tttcgccaag cggcagcttg cattcgtggt
     1741 tcgcgctgca cttgccgcac ggccccatct gggcgcagtg cgaatcgtcg atgcacttca
     1801 cgcacttttt gtcatagcag tattcgccct tttccttgca ctgcttgtcg ctgtcgcagt
     1861 tcgggtactt gggcccgcag gcgacaaggg tcagaaggga cgtaattgcg aaaacgatgg
     1921 tcaacactct ggccagtttc atgaaaacct cctgaaatat gcggggaaag tactttcctg
     1981 cgcacggaac aaagaccatg cgcaagatta ttaccggaag cctgcccgtg tcaagaacta
     2041 cggcagcctg cgggccgttc cgcaggcttt gatttgagaa attcgtccgg cactcgtcgg
     2101 tcgtcttctt ggctgcaccg cgatatcggc aatacaatcc atgcagggaa gtggtgccgg
     2161 gatgccggac tgtcccttcg tttcgggaaa atgcagtgtt caaggtgggt tgaagatgaa
     2221 gttcgcgcca gccgtcatcg tatgtctcct gctgtcggcc atgcctggct gcgccggcaa
     2281 tatcgataag atgacggttc gtttcgacga gatggatatg agggttcaga ccctttcacg
     2341 aacgaacaac gcgcttcggg gaaggattga cgaactggaa aacaggattc tgctgcttca
     2401 ggacgaagtc gagacccagc gccttgccgg catgcgcggc ggctcacagc catacgtgac
     2461 gccatcctat tcggcacctg ccgcgccagc ttccagcctg cccgttatga aacttgaacc
     2521 tggcgacgcg gcgccggaac cggtcgattt cgtcgcgggg tcggacactt ccgagcctta
     2581 cccgaccgat tatgcattcg aggattacga ccgcgctgtt caggctgccg agccttcggc
     2641 cgagcccatt gatggcgggt cgcttgatat cgccgattcc tatcagggaa ttgacgacat
     2701 gggccgggtg gtgccgtcaa agtcgaaggg ttcgtcaaag gtggcgaaga gtactgccag
     2761 gacggagaag aaggcgcctc caccgccgcc ggcaaggaag gaaaagcctg cggatgtcgc
     2821 caagaaggcg gcggccatgg cgcttgaaca gtacaaggcc gcctacagct tgtacggcga
     2881 gggtcggctt gacgatgccc gcgaggcgtt caaccagttc gtcatcgaca atccgcgtca
     2941 cgcctatgcg gacaacgccc gttactgggt gggcgaatgc tggtacgacc ggcgcgaata
     3001 cgaaaaggcc agggtggagt tcatgcgcgt cgtgaccgaa tatccggacg gcaacaaggt
     3061 tccggacgcg atggtgaagg tcggtctttc ggatcagaac cttggaagga tgtctgacgc
     3121 aaggcgcatg tacgacgctg tcatcctgac ttatccggac agcgaggctg ccgccgtggc
     3181 gaccaggctg atcggcaaga tgcagtagcg accggcgtcc cggatcactg gccgggccgc
     3241 ttgaaaacgg tttccaggga ggaattcatg aacgcaggat ttcttgaccg cgtgaccagg
     3301 gcaatcctag tagtctccgt cgggctcctg tccgcgattc gggtttcagg cgcagtggcc
     3361 caggaaatcc aggtcagcgg cagcatcgac gacgtcaacg tctatgtcgc ggaaggcggt
     3421 gactcgctgt gggacatcgc ggatcggttc ttttccgacc cgtggtactg gccgaccttg
     3481 tggtcgttca acccgcacat cacgaatccg aactggatct tcccgggtga cagggtatat
     3541 ctggtcccgc cgaagccggt gaaggatgac aagggcgggt atgcaatgtc cgaatccagg
     3601 ttcgatccag gcgtcaggga cgaactggcg cttggccgac gagtcggata catgtcggag
     3661 gacgaggtca aggcgtccgg cgtcatcagc cattcccgcg aggaaaagga aatgctcggg
     3721 gaacgcgacg acatctatat caagttcgac actgcacgtc gcatcaaccg cggcgacctg
     3781 gttcttatct atcgccaggg caagagggtt aagcatccgg tcacgaagaa gaagcttggg
     3841 ttcgaaatca agtatctggg aatggccaag gtcaccgaca cggaaaagga catgaacaag
     3901 gccgtcatca tgaagtcgtt cgaggagatc tatcgaaacg acaaggtgat ggcgttcgcg
     3961 cctgttcaga agatggttcc gccggtttcc aactccaagg aagtggtcgg atcaatcatc
     4021 gcctcgattg agggtgtaag gttccttggc gagtatcatt acgtgatgat cgataagggt
     4081 tcgaaggacg aggtcatggc cggcaaccgt ttcatggttg tgacgaccgg ggacggcgtc
     4141 gatcggtaca acaagaaaat caagaagaag gcccgcaagg aattcccgga agagaagtat
     4201 ggcgagcttc tggtaatcga agcccgcgac agttcgtcac tctgcatcgt gacgtacgcc
     4261 aaccgtgaat tcgaagtcgg ggccaaggtc ctgatgcctg aaggctattg aaaacaatcc
     4321 cgtttcccag tctttacttt cgccaaatca atgcaaatgg tagtttccct ggtcgttcag
     4381 gattcggatc gaggggttcg ctgataatac tcgcgaagac gcaaggggtt cccgatgcaa
     4441 aggaaagtgg tcatcatcgg ttccggtccg gcgggcctta cagccgccat ctatgcttca
     4501 agggcgggtc tttcgccatt ggttctggaa gggccggctc ctggcgggca gcttctgaca
     4561 accaccgaag tcgaaaattt tcccggtttt gagaacggcg tttccggtcc ggagttgatg
     4621 gacgcgatga ggcgccaggc gaccaggttc ggcgccgaat gccgcttttc atcggcgacc
     4681 gcggtcgatt ttgcgtcccg ccccttcatg gttgagcttg acggttccga gacggttcag
     4741 gccgactcgg tgatcatcgc gacaggggcc acggccaggt atctggggct cccgagcgag
     4801 acggccctgc gcggtcatgg tgtcagcgcg tgcgcggtct gtgacggttt cttcttccgt
     4861 ggaaaggacg tggcggtgat tggcggcggg gatacggcgc tggaagaggc ctgttatctg
     4921 accaggtttg ccaaccgcgt tttcctggtg caccgaaggg atcagcttcg cgcgtcgaag
     4981 gccatgcagc agcggataat ggccaatccg aaggtttcgt tcgtctggaa ttccgtggtc
     5041 gatgaagtca tttcggacga cgaaggcaag tcggtcgccg gcttgctgct gcgcgatgtg
     5101 aaatccggtt catccagtgt tcttgcggtt caggggatgt tcctggggat cggtcacgca
     5161 ccggctacgg agatattcaa gggtcagatt gatctggacg accagggcta tataaggctg
     5221 tctcatcaca ccagcacttc gattgaaggc gtttttgcgt gcggtgacgt ggcagattcc
     5281 cgttacaggc aggcgacgac ggcggccggg tcgggcgcgg cggccgcgat tgattgcgaa
     5341 cgatggctgg cggatcaggg cgaatgaaag acgcgggccg atgggattgg ttcatggatg
     5401 gggtaagggc cgcgatcgcg gacgttcccg gtccggccgt ctcgatgaac ggtgcgatta
     5461 cgtcaagccc gttcaaggtg cttgtggcga ccctgataag ccttcgaacc agggatcagg
     5521 tgaccggccc ggcgtctgaa cgcctgttcg ccctggcgga ctcgcccgag gccatgattg
     5581 cccttggggc cccggccgtt tccgccgcca tcttcccagc gaacttccat ccgacaaagg
     5641 ccgaacggat cgtcgaggtc tcacgcctgc tgatcgaaag gcacgccggg caggttcccg
     5701 cggacatgga cgccctgctg gctcttccag gcgtcggccc caagacggcc aacctggttc
     5761 ttgcggaagg tttcggcatc gacgccatct gcgtcgatac ccatgtccac cggattccca
     5821 accgggttgg cctgattgac acccgggatc ccgttcagac cgaagaagtc ctgagaagcc
     5881 ggcttccact gaagtactgg aaggaaatca accggattct ggttcctttc ggtcagtcgg
     5941 tgtgtacccc ggtttcaccg aagtgttcaa tctgcccgct gattgaaggg tgcgcccgca
     6001 ggttcaaggg gccatcgcgt taaggccgcc acaatcggcg ccgtcccccc tttactttaa
     6061 gtagtcagtc gataaaatga cgcacgcccc cgggggcatg tttgactcat aacggaacac
     6121 tgggaggttt cagatggctg gtcatccgag gaaggtcgta atcgtgggag ccgctggtcg
     6181 cgatttccat aacttcaact gtctttaccg ggataacgac gcgttcaggg ttgtcgcttt
     6241 cacggcggcg cagattcccg atatcgctgg caggaagtac cctgcagtcc tggcgggcaa
     6301 cctttacccg gatggcatcc cgatcgtcga ggaatgcgaa ctcgaaagcc ttattcgcga
     6361 acaccagatt gacgacgtcg ttttctctta ctccgacgtg tcccaccaga ccgtgatgga
     6421 aatcggtgaa agggcgattg ccgccggcgc caactatgtg ctggccggtt cccgtgacac
     6481 cgaactggtt tcgaagaagc cggtcatcgc cgtctgcgca atccgcaccg gttgcggcaa
     6541 gagccagacg tcgcgctata tcaccgacat ccttcacaag atgggcctga aggtcgcggt
     6601 catccgccac ccgatgccgt acggcgacct ggccgttcag gccgtccagc gattcgccag
     6661 ctatgacgac cttgcgaagc acaagtgcac gatcgaggaa cgcgaggaat acgaacccca
     6721 catcgaagcc gggtcgatcg tttacgccgg cgtcgattac cacgcgatcc tgaaggcggc
     6781 cgaggccgaa gccgacgtca tcctgtggga cggcggaaac aacgacatgc cgttctacaa
     6841 gccgaacctt ttcgtgacgg tgctcgatcc gctgcgcccc ggacacgaag tcggttacta
     6901 cccgggaatg gcgaacctgc tgatggcgga cgtcctcgtg atcaacaaga tcgactccgc
     6961 gaccgcggaa gatcttgaag tcgtcaaggc atcggtggcc aagtggaacc cgaaggcccc
     7021 gatcgtcgaa gcggaatcgg tcgtatccct tgatgagcag gtcgaactga agggcacgaa
     7081 ggttctggtc gtcgaggacg gcccgaccct tacccacggc ggcatgaaga tcggcgccgg
     7141 ctgggtcgtc gcgaagcgtg gcggcgcgga aatcgtcgag ccgctgaagt atgcggtcgg
     7201 ttcgatcaag gccacgtacg agaagtaccc gcactgcgcc ggcatccttc ccgcgatggg
     7261 atactcggaa tcccagcttg ccgaccttaa cgccaccgtc aacaagatcc cggccgacct
     7321 gatcatcgtc ggaacgccga tcgatcttcg caagctgctt aaggacgacc gcaagatggt
     7381 tcgcgtccgt tacgacctga agccggttcg cggtccggag cttgccgact tcgtgaagaa
     7441 ggtcgtcgcc tgaccagact aaccgaaagg agcccaaatg acttcaattg atgccatcgg
     7501 caagcttgag tccaggttcg acaagctggt taagccgctg gacctgttca tgctgatcga
     7561 gcatgtccgt cgcctggatc cgatggcctt cgcaaggcat ttcaagggat acaggccgca
     7621 gatgcttggt cgcaagcgcg tggtcacggc gctggcgttc gaaattttta ccaggaagaa
     7681 cgaggtgatc ggggacatca tcacccttct gtggaaccag accaggcgag acgtctatga
     7741 cgccatgcat gaactggttt caacaatcag cgaggacgtc gagtcgatca ccgagattcc
     7801 cgacgaaagg gcccacgcct tcatcgacgt cctgcttgaa gagttcgacc gcgacgaaat
     7861 ccttatctgc gtgcggatga acgatgtccg cttcaacgag ccggtcatcg ttgccagact
     7921 ggaaaatggt gaacccgcgc ccgcagaggc ggccgcgccg gaagcggcga atcccgcggt
     7981 tgtcgaagcg acggccgagc ccgtcgcaat cgactcttcc aaccaggccc cgaaccctga
     8041 ataatcgctt gcacaacatc acgcaaaccc tgtagaatcc tttattcccg gtcatcgggg
     8101 ggcttttgcg gattcccgca aaaagatttg acattggggg ctgcggtatc tgataatcgt
     8161 aaagcggttt tccggaggat taattatggc ctcgaaacga gtgcccacct tcaagaagcg
     8221 gattcaggac ttcctctcga gcgaagaggg aaagatcacc cgtggacagg ctgctgctat
     8281 cggctcattt atgctgatgg ccggcaccag tggccaggcc ctggccgcag gcgatgctga
     8341 tcaggctgcc gctgcatccg atttcggaac gcagacgcac tgctcgcacg cgtcgcacgg
     8401 ctcgcacggc tcgcacggct cgcacggctc ccatgcttcc catgggtcgc acggctcgca
     8461 cggctcgcac ggctcgcacg gctcccacgg ttcgcatggt tcccacggct cgcacggctc
     8521 ctggtaagcg acgaaacggg gttcgtcgaa cggaaccgca gctcgggggg gcttgccgga
     8581 tcaacggtcc gtattgattc ctgttcgcgg gatcacgtct tttctttacc ctgaattttg
     8641 aatccatcac aagacgcgtc gcgccgtttc attcttgtcc tccggcctgc gtccagggcc
     8701 aggtcgtgtg gatcacctgt ctgttgctct gtgaccttgt ctccagcctg cgcccggggc
     8761 caggggcgaa aaaaattttg ttgatttttc cgatggcggg tatgtttttg tgcgcgaacg
     8821 cgtacgttaa ggagttttcg tgggcaaacc gctgataatc gtcgagtcgc cggcaaaggc
     8881 gaccaccata aagaagtatc tttccggcaa gtacgaggtc gaggcatcgg ccggtcacat
     8941 caaggacctg ccgcagagct cgctgggcat cgacatccag aagggcttcg cgccgaagct
     9001 ggaagtcgtg cgtggaaaga agaagattgt cgatcgcctg aagaaggcgg catcgacggc
     9061 cgatgaagta tttatcgcaa ccgaccccga ccgtgaggga gaggccatag cgtggcatat
     9121 cgccgaggaa ctgaagaagg tggttccatc ggcccgccga gtcctttttc acgaaatcac
     9181 cagagacgcc gtcaggaagg cgctggagca cccaagggat ctggacgtgc tgatgtacga
     9241 atcccagctt gccagaagga cgctggatcg gctggtcggt tatcagattt ctcccattct
     9301 gtggaagaag gtgcagggcg ggctttcggc cgggcgggtt cagtcggtcg cggtcaggct
     9361 ggtggtcgaa cgtgaacggg aaatcgaggc gttcgactcg aaggagtact ggctggtcca
     9421 cgcggttgtt cacaaggacg aggcccagtc aaaaccgttc aaggctcttc tgcgcaagat
     9481 tgacgggcgc aaggccgaac tgtccggcaa ggccgttacg ttcgaacttg ttgaaaggct
     9541 tcgcgaggcc agtttcagga ttgccagcct ggtcaggaag gatcgcccaa gggcgccatc
     9601 gccgccatat ataacaagca gcctgcagca ggacgcgtcg cgccttctgc acttctcgcc
     9661 gtcgcacacg atgtcggtgg cgcagcggct ttatgaaggc gtcgaccttg gcgacgaagg
     9721 ccgcgtgggc ctgattacgt acatgagaac ggactcggtg cgactgtccg cggattcggt
     9781 gacagaggcg cgctcggtga tcgcgtcaca gttcggtccc gactacgttc cggcccggca
     9841 ggttgagtac aggaaccgcc gcagcgcaca ggatgcgcac gaggcgatcc ggcctacttc
     9901 cgccgaaaga actccggcat ccctgaaggg agtgctggaa cgcgacgaac agaaacttta
     9961 tgcactgatt tacaaccgct tcctggcgtg ccagatgcgt ccggccatat ttgaccagac
    10021 aacggtcgat atcgaggcgg ccggggccga gttcagggcc gccggctcgg tgatgaagtt
    10081 cgacggctgg ctggcggtct ggaggctgga caagtcggcg aggctcgagg atgtcgagac
    10141 ctcggaaccg gaagatgagg gcgatggcgg acagcggctg cccagttcgc tggtggaagg
    10201 cgatgcgctg ctgcttgacg accttctgac cgagcagaag ttcaccgagc cgcccccgag
    10261 gttcaccgaa gccacgctgg tgcgggaact cgaggaacgc ggtatcggcc gtccgtcaac
    10321 gtacgcgtcc atcgtttcga caatccagga caagtcgtac gtctacaagc atgagggcag
    10381 gcttcgtccg tccgagcttg gaaggatcgt caacgatctt ctctccgggc atttccgcgg
    10441 agtcgtcgat tacgatttca cggccaggat ggagcaggat ctggatttga ttgaggaagg
    10501 gcgcctggac aggcttgaac tgcttgaaaa gttctatctt gagttcagca ggactcttga
    10561 gcaggcccag ggcgagatgc ggtcggtcaa ggtcgatgcg gttccgtccg gggttcggtg
    10621 tcccgagtgc tcaaaggaac tgctgataag gcttggcaag aacggcgcct tccttggttg
    10681 cgaaggctat cccgaatgca gggtgacccg cgagttcgac cgggacgtga agggccacgt
    10741 ggtcgtccgg gaaagccccg acgtgggaac ctgtccgaca tgccaggcgc cgctgcagat
    10801 caggtccggc aagttcggga agttcgtggc gtgcagccgc tatccggact gccgtttcac
    10861 catgccgtat tcccgcgggg aacgctgtcc ggtcgatgga tgtgccggca ggcttgttga
    10921 gcgacagtcg aagaagggaa agaagttctt ctcgtgcagc gcgtatcccg gctgtcgttt
    10981 catcacaagc tacgagccgc ttgaggagac ctgtcccgag tgcggcgcgc cggtgctttt
    11041 cgtgcgcagc ttccgcggca cgaggacggt gatgtgccag cgcgaaggct gcggattcac
    11101 caggcgggtt cttgcaagaa aggccgccgg ttcggggcag ggctgatgga gaatccgcgg
    11161 caaaggtcgg gtgattcggg catgaccagc cttggcgacg gaaccatggt ctcgaaaggt
    11221 tccgaccggg tgtccgccta cggtgcgatt gacgaacttg actgcttcgc cggccttgcc
    11281 agggtcgagg tgcttgagtc gctgccgccc gggcccgacc gtgatctggc ggttcgttcg
    11341 cttgagaccg tccagaacgc atgctggctt cttgccgcat ctctcgccgc gccgggacgg
    11401 gaatgtcctg aagccccgca gatccaggcc agcatcgagc agcttgccgt tgaaatggaa
    11461 cgggcggccg gccctgtctc ggggttcgta atcccgggtt catccaggtc ggaagcgctt
    11521 ctgcacgttg cgcgggctgt ctgccgcagg gccgaaaggg aggttgttga acttttcggc
    11581 gtcctgggcg acggcgcagc gatcggatgg gaactggcgg tgctgaaccg catgtcgagc
    11641 ctgttgttcg ccgcatcaag gctggcgctg aaggccaggg gaattccagt atcgtaccgc
    11701 ggtcgatgat ggttcgtgtc gtggcacagg tcggacgcgg atcggtatca aggaacctgt
    11761 tgacaaccgt cgaggatgga aataaaatgc ttcccgcttt gattgctgcg aaagtggcgg
    11821 aattggcaga cgcgctagat tcaggtt
//
LOCUS       DAOPPA010000031        11782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6282_length_11782_cov_4.253688, whole genome shotgun sequence.
ACCESSION   DAOPPA010000031 DAOPPA010000000
VERSION     DAOPPA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6282_length_11782_cov_4.253688"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PKH54_01830"
     CDS             <1..1302
                     /locus_tag="PKH54_01830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC98656.1"
                     /translation="DDLFEVTGGKEKNLTAKNLASDRAPRYSPDGKFIAFLSQEKPGY
                     ESDRILLKVIDRKRGEVVSLTDALDNWVMDFVWAADSQSLIATVAREGHRVLYRVSLN
                     KRTGFTPMFDSGMYRNLILSADGKTLFHTRETITTPPEVYAMDLATGAARQLTNLNGE
                     ALKNLLLPEIEQIRWDGAKVDGVPQRVHGFLARPVGADGKALKKKVPLVVMVHGGPQG
                     AWENSMHPRWTPLGLTGNGYAVAMPNPTGSFGYKQDFVNKVSTDWGGRAYEDIMGMVD
                     YLSKDSLIDGSSVAAMGGSYGGYMANWMEAKAGTRFKCLISHAGPADLEIMYGTTDEL
                     WFPEYEFRGPPWAEPEEYRKWSPLSYVMDFKTPMLVMHGANDFRVPLEQALAMFTALK
                     RRNIDSRLVVFPDEDHFVNTPKNRRFWYRTVNEWLDKYLKK"
     gene            1371..2024
                     /locus_tag="PKH54_01835"
     CDS             1371..2024
                     /locus_tag="PKH54_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98657.1"
                     /translation="MRRSVLKNAIAAMSDDDILALAGYLVCRTDADDSRQVWLDFVRE
                     ILLTPPWSAERMGLVASYPAIRERRPVLEFLGIDDERCRAGGGRFGQWAMEDIPLGVR
                     KAKARLSNRDTLLQLTQDPDPSVARILLDNPFVTEVMALRVTTKRPQKAQILRGVMES
                     RFITSETVQNSLVNNPYCPTRMAVALVPLLSRTHRAETADSGSVDEAVRNAAAAMNL"
     gene            complement(2038..3144)
                     /locus_tag="PKH54_01840"
     CDS             complement(2038..3144)
                     /locus_tag="PKH54_01840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC98658.1"
                     /translation="MAYVPGTRLMQTLPLLFLACAASIGCRQEIPQRPSVIEPPEILV
                     DSPEWNGVGDDEIVARAGDVAIPAWRVREAFSRADAGADPQEVLDSVLVEELLARKAV
                     DGRGFVPERSVFERALASRYVEKKIVQEFQPQDVPEGMLKEMFALPQVWARFNHVDLY
                     DVQDYQWICCSDPKSCDPAVFEECMNEGQAPMAAVRQMLEQDPPEVADMAIFAPEFKK
                     RAYHLTYQEYSFAFDRKAGYQKGLTHIDSTLADEVMKLSPGQFTSPVRSRFGWHIARL
                     ISHLPEASGDLQDPRVRLEIATFFMQRFRQDHFLNHLASLIGPNSLKLLQIYYENRPA
                     PQGKPVYDVKLFYNALRDAAASEAEQKELEPPLL"
     gene            complement(3173..4831)
                     /locus_tag="PKH54_01845"
     CDS             complement(3173..4831)
                     /locus_tag="PKH54_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC98659.1"
                     /translation="MGIFRVDVLAILAVSFLMAGCGGTPQDQDVEADDGGIDIVIQID
                     TGNDQGGHDLANPDVGSDVSETSTPDIPVEIPPDEVPPLVVATYPMNEETGIPTDFVV
                     RVTFSEDMRAVFLSADFLKLFDVYGQSVPGTIEYDATSFTVSFTPESTTQIQSLAPYS
                     FRISGSVQDLAANELGSDYTFRFSTRGPDGMDGYREIASRYAPRLYQATQILAPQWDY
                     PTSVNFDLDWNMDNNVPSMEAAQSLPIWVYYDVIETYTHYFIRYMYYYARHDDSTFVF
                     GNQVSGTMVVVQKDPEEPIAVETYFGNGGFEDIRSFVTTESGLVIDDGNGNYNDGDRR
                     KYNANWVFPKDQLFPGGHFQNYITSKSHESCAWIQVTKEDSLDFKCQLDSAMKSTLNI
                     MHFSHKGTDAETLNTPFAYKNSEGSDVGYGLRLAIEDWWVRRDRIDEGQMFGATIDYV
                     PPQGMAGSGMVLPGNFRPSPTGTTAGGKSPWRWEWEPATMNAYLYYTEFSEGTFFIHP
                     AYYFAQRHRLTPTADASGFSASYCYNPYLQIDKRGTTADCSSIN"
     gene            5093..6981
                     /gene="dnaK"
                     /locus_tag="PKH54_01850"
                     /pseudo
     CDS             5093..6981
                     /gene="dnaK"
                     /locus_tag="PKH54_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(7258..7887)
                     /locus_tag="PKH54_01855"
     CDS             complement(7258..7887)
                     /locus_tag="PKH54_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98660.1"
                     /translation="MKKLVLVLTVAMSLVAFTACKEKFPTPGDSFKAWMTAIEKCDVA
                     GIKGGLTAESVEQLTQISEQLKNFAQKPEGQAEDFDIFKEMCKGFSKAGGMEVISETV
                     SPDNKDEARVVYKTNGKENSAPMVRTEKGWAIDMARMMKEAMQAQMEKMKAMAPPPAP
                     EGAPAAEGAAPAPEAAPAAEGAAPAPEAAPAAEAPAPAPAAEAAPATQN"
     gene            complement(7914..8531)
                     /locus_tag="PKH54_01860"
     CDS             complement(7914..8531)
                     /locus_tag="PKH54_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC98661.1"
                     /translation="MWWVPTVIEHTARGEREWTVFSRLLKDRIIFLGWEIDDNLASVV
                     IAQLLYLESEDPDKDIYLYINSPGGNVNSTLAVYDTMQYVRCPISTICIGQASSMSAL
                     LLAAGTPGKRRALPHSRILLHQPLGSFSGQATDVDIRTREILFLKNQVVKLLSNHSGR
                     SVEQILKDSDRERFLSPEEAREYGLIDEVIDQNSVPGGVSGTKHP"
     gene            complement(8575..10083)
                     /gene="tig"
                     /locus_tag="PKH54_01865"
     CDS             complement(8575..10083)
                     /gene="tig"
                     /locus_tag="PKH54_01865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOC98662.1"
                     /translation="MKVDHEDLGKLTHRITVAIDKEGFDKERRRLARKYAAQVRVPGF
                     RPGMAPMHLVLAQLGPRIEYEVRENLISQTFGDAIDQFKLKASTEPKFDLVRDEENAL
                     EYTAEIEVFPEVDVKDYLEVEVAGPFAPEVTEAQIMERIDGLRKSLSRFEDKGEGCVV
                     EEGDMAETQVTLSLVSDGTVIKDAHSSRIMGGTDDEPVEGIGRQILGLAAGEEKTVQG
                     RIGRITARGQKMDPDQAGDVRAVVKIEKIRARKMPELDNEFVKKHADVETVEEFHALL
                     KKQLEEQRDSKLKEETEELILSRICENNPFEIGERTIERLADMAEKEARDRVLQQIPE
                     EKRAELAKDFDLGVPREKSLEEARRNLTRSIILEAIADKEGIQVTEADIEAKLQETAD
                     AYGMPLPKIKAIFGQERLEQLGRQLRVGKTLDLLTRHAVVTPGAEGAVQAAAAPTAED
                     KPARKATRTKKTAEKQDPVEETAAPAEDAAPEAAKKPARKPAAKKAAAPKQD"
     gene            complement(10147..11574)
                     /locus_tag="PKH54_01870"
     CDS             complement(10147..11574)
                     /locus_tag="PKH54_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98663.1"
                     /translation="MTLGTNHGARRALFVYTSAQAEYLAAARHAELSMKKVPHLGFLY
                     LAAVLKENGIDCQVMDRTLDRFGPDELAAKARNEGLVFVGFYSDSITRDAVCHWIVKL
                     RAAAPELPILVGGADAYRPGPYLNAGATMVCTGEGEEAIVEMIRAIDGELDPREIDGI
                     AFSVDGSIVINPARQPIADLDSIPFPRRDIIDIDRYHDWRVLGMRKPYTTMITSRGCH
                     RRCTFCSVPFVSGRTVRLRSAANVLCEIDELVRDFGIRYITFKDDYFAVDPDWEREFC
                     EGLIERGYDLKWTCQTHPSVFEKDRGQRLERFRRAGCDLLIFGLQSTDPDILKAIRRS
                     PREPEIVAANVAAARKIGLQTVVEFICGLPGWNRATLEADVRFALKVRPQYAAFYPLM
                     RLDPSELFEQFGIDGDMCGLSNREINRLGAWAYRRFYLDPRILFPTAWFVLKDNPLWI
                     RHGLGFLAGQLFPWFRPAGRLAKVR"
BASE COUNT         2299 a         3453 c         3558 g         2472 t
ORIGIN      
        1 gacgacctgt ttgaagtcac cgggggcaag gaaaagaacc tgaccgcaaa gaacctggct
       61 tccgaccgcg cgccgcgcta ttcgcccgac ggaaagttca tcgccttcct ttcgcaggaa
      121 aagcccggct atgagtcgga tcggatcctt ctgaaggtca tcgatcgcaa gcggggcgag
      181 gtcgtttccc tgaccgacgc ccttgacaac tgggtgatgg atttcgtctg ggccgcggac
      241 agccagtcgc ttatcgcgac cgtggcgcgg gagggtcacc gggttctgta tcgcgtttcc
      301 ctgaacaagc gcaccggctt cacccctatg ttcgacagcg gcatgtaccg caacctgatt
      361 ctttcggccg acgggaagac ccttttccat accagggaaa cgattacgac cccgcccgaa
      421 gtgtatgcga tggatctggc aaccggggcc gcccgtcagc ttacgaatct aaacggtgag
      481 gcgctcaaga acctgctgct tcctgagatc gaacagattc gctgggacgg cgcgaaggtc
      541 gatggcgtcc cgcagcgtgt tcacgggttc ctggccagac cggtcggcgc cgacgggaag
      601 gctttgaaga agaaggttcc gctggtcgtc atggttcatg gcggacccca gggcgcgtgg
      661 gaaaattcga tgcatccacg ctggaccccg cttgggctga ctgggaacgg ctatgcagtg
      721 gcgatgccga atccgaccgg aagctttggc tacaagcagg atttcgtcaa caaggtctcg
      781 accgactggg gcggacgcgc gtacgaagac atcatgggaa tggtcgatta cctttcaaag
      841 gattcactga ttgacgggag ttccgtggcc gcgatgggtg gttcgtatgg cgggtacatg
      901 gccaactgga tggaggcgaa ggccggcacc aggttcaagt gcctgatttc gcatgcgggt
      961 ccggcggatc tggaaatcat gtacgggacg accgacgagc tctggttccc cgaatacgaa
     1021 ttccgcgggc cgccctgggc cgagcccgaa gaataccgga agtggtcgcc gctgtcgtac
     1081 gtgatggact tcaagacccc gatgctggtc atgcacggcg cgaacgactt cagggttccg
     1141 cttgagcagg cacttgcaat gttcaccgcc ttgaagcggc gcaacatcga ctcccggctg
     1201 gtcgttttcc cggacgagga ccatttcgtc aacactccga aaaacaggcg tttctggtat
     1261 cggacggtta acgaatggct ggataagtat ctgaagaagt gatcaacggc aatccaggct
     1321 tcctgccatc agagttttcc cgccgcctgc gggcgtttcc cgaccacctg atgcgaaggt
     1381 cggttctgaa aaacgccatc gccgccatgt ccgacgacga tatactggcc ctggccggat
     1441 atcttgtctg tcgcacggac gcggacgatt cacgccaggt ctggcttgat ttcgtaaggg
     1501 aaatcctgct gaccccaccc tggtcggccg aacggatggg gcttgttgcc agttatcccg
     1561 ccatccggga gcgtcgtccg gtgcttgaat tcctgggcat tgacgatgaa cgttgccgcg
     1621 ccggtggcgg tcgattcggc cagtgggcga tggaggacat ccccctcggc gtgcggaagg
     1681 cgaaggcaag gttgagcaac agggacacgt tgcttcagtt gacccaggat ccggatccgt
     1741 ccgtggcaag gattcttctg gacaatccgt tcgtgaccga ggtgatggcc ttgcgggtca
     1801 ccacaaagcg tccccagaag gcccagattc ttcgtggtgt gatggagtcc cgcttcatta
     1861 cgtccgagac ggtccagaat tcgctggtga acaatcccta ctgtcccacg cggatggcgg
     1921 tggccctggt tccgctgctt tccaggacgc accgggccga aacggcggat tccggcagcg
     1981 ttgacgaggc cgtgcgaaat gccgcggctg cgatgaacct ttgaatcaag aacaaggtca
     2041 cagaagcggg ggttccagtt ccttctgctc ggcctcgctt gcggccgcgt cgcgcagggc
     2101 gttgtagaac agcttcacgt catacacggg ctttccctgc ggtgccggcc ggttttcgta
     2161 gtagatctga agaagcttca ggctgttcgg cccgatcagg ctcgcaaggt ggttcaggaa
     2221 atggtcctgt cggaagcgct gcatgaagaa tgtggcgatt tccaggcgaa ccctgggatc
     2281 ctgcaggtct cccgaagcct ccggcagatg gcttataagc ctggcgatgt gccacccgaa
     2341 tctggatctg accgggctgg tgaactgccc gggcgacagt ttcatgactt cgtcggcaag
     2401 ggtggagtcg atgtgggtaa gccccttctg gtagccggcc ttcctgtcga aagcgaacga
     2461 gtactcctgg tatgtcaggt gatatgcccg cttcttgaac tcgggcgcga aaatcgccat
     2521 gtcggcaact tccggcggat cctgttcaag catctgcctg acggcggcca tcggcgcctg
     2581 gccctcgttc atgcattcct cgaacacagc cgggtcgcat gatttcgggt cggagcagca
     2641 gatccattga taatcctgga cgtcgtacag atccacgtga ttgaaacggg cccagacctg
     2701 cggaagggcg aacatctcct tcagcatgcc ttccggcacg tcctggggtt gaaactcctg
     2761 gacaatcttt ttttcgacat acctgctggc cagcgcacgc tcgaagaccg acctttctgg
     2821 cacgaatccg cgtccatcga ccgccttgcg ggccagcagt tcctccacca gcacggaatc
     2881 aaggacttcc tgtgggtcgg ccccggcgtc cgcccgggaa aatgcttccc tgacgcgcca
     2941 ggccgggatg gccacatcgc cggcgcgtgc aacgatttca tcgtccccaa ctccgttcca
     3001 ctcgggcgaa tccaccagga tttcaggcgg ttcaatgacg gaagggcgct gcgggatttc
     3061 ctggcggcag ccgatcgacg cggcgcaggc cagaaacaga agcggcaacg tttgcatcag
     3121 tcgcgtgcca ggcacgtaag ccatcagttc aagcccccag aacccgaaga gatcagttaa
     3181 ttgaagagca gtcggcggtt gtgccgcgct tgtcaatctg cagatacggg ttatagcagt
     3241 acgatgccga gaatccggac gcgtcggcgg tcggcgtgag cctgtgtctt tgcgcgaagt
     3301 aatacgccgg atggatgaag aaagtgcctt ccgagaattc cgtgtagtag agataggcgt
     3361 tcatcgtggc tggttcccac tcccatctcc aggggctctt gccgcctgcc gtggttcccg
     3421 tcggactcgg cctgaagttg ccgggcagca ccattccgga tccggccatt ccctgggggg
     3481 gcacatagtc gatggtcgcg ccgaacatct gaccctcgtc gatgcggtcg cgccttaccc
     3541 accagtcctc gatcgccagg cgcaagccgt accccacgtc gctgccttcc gaatttttgt
     3601 acgcgaacgg tgtgttcagc gtttccgcgt cggttccctt gtgggagaaa tgcatgatgt
     3661 tcagcgtcga tttcatggcc gaatcgagct ggcacttgaa atcgagcgag tcttcctttg
     3721 tgacctgaat ccacgcgcat gattcgtgcg acttgctggt gatgtagttc tggaagtgtc
     3781 cgccagggaa gagctggtct ttcgggaaca cccagtttgc gttgtatttc ctgcgatcgc
     3841 cgtcgttgta attgccgttt ccgtcatcga tgaccagacc ggactcggtg gtcacgaagc
     3901 tgcggatatc ctcgaagccg ccgttgccga agtatgtctc gaccgcaatc ggttcctcgg
     3961 gatccttctg gacgacgacc atggttccgg agacctggtt tccaaacacg aatgtcgaat
     4021 catcgtgcct ggcgtagtag tacatgtacc ttatgaaata atgggtatag gtctcgatga
     4081 cgtcgtaata cacccagatc ggcaggctct gcgcggcttc catcgatgga acgttgttgt
     4141 ccatgttcca gtcaaggtcg aaattgaccg acgtcgggta gtcccactgt ggcgcaagaa
     4201 tctgggtcgc ctggtacagc cttggtgcat atcgggacgc gatttcgcga tatccgtcca
     4261 tgccgtccgg gcccctggtg gagaacctga acgtgtagtc gcttccgagc tcgttggcgg
     4321 ccaggtcctg gacgcttccg gagatgcgga agctgtaagg ggccagcgac tggatctggg
     4381 tcgttgattc aggcgtgaat gaaaccgtga acgatgtcgc gtcgtattcg atggttccag
     4441 gaaccgactg gccataaacg tcgaaaagtt tcaggaaatc ggcactcaga aacaccgccc
     4501 gcatgtcttc gctgaaagtg acgcggacga cgaaatcggt cgggattccg gtttcctcgt
     4561 tcatcgggta tgtcgccacc acaagggggg ggacttcgtc cggtggaatt tcgaccggaa
     4621 tgtcgggcgt cgaagtctcg gacacgtcgc ttccgacgtc gggattcgca aggtcatggc
     4681 caccctggtc atttccggtg tcgatctgga taacgatatc gattccgcca tcatcggctt
     4741 cgacgtcctg atcctggggc gttccgccgc acccggccat caggaaagaa acggccagaa
     4801 ttgccagcac gtcaactctg aaaataccca taagtctgtt catccgcagg atcctccgtg
     4861 ttgcccggcc ccgtctggcg gcaaatgtcg atgaagtgcc ggaacactgg aataaagaca
     4921 tgcaaagact cgcaaaaacg gccctggatg tcaattggat gctgctgtcg tcaaccccgt
     4981 ccgaaacatt gacaagaccc cggttgactt gttgccgcgc gtgattattt tcctgtccga
     5041 accgggccac gggcccctgt gacaatggat tctgattcgt ctggaggcgg acatggaaag
     5101 gataatcggt atcgaccttg gaaccacgaa cagtgtcgtg gccgtgatgg aagctgggga
     5161 agccaaggta atccagaacg aggaaggcgg gcgtacaaca ccgtccgtcg ttgctttcga
     5221 caacaagggt gccgtgcttg tcggccagat tgcgaagcgc caggcaatca cgaacccgga
     5281 aacacgatat tcagcatcaa gcgcttcatg ggccgtcgct ttggcgaggt cgatcaggaa
     5341 atgaagttcg tgccttacca cgttcggcgc ggtgaaaacg atcaggccgt cgtcttggca
     5401 cagggaaagg atgtaacccc gcccgaggtt tcggccagga ttcttcagaa gctgaagaag
     5461 gccgccgagg attatcttgg cggtccggtc agtgcggccg tcatcacggt tcccgcgtac
     5521 ttcaacgatt cgcagcgcca ggcgaccaag gatgcgggcc ggattgctgg acttgaagtc
     5581 aagcgcatca tcaacgagcc gacagcggcg gcgcttgcgt acggaatgga caagaagaag
     5641 gaccagacaa tcgccgtcta tgactttggc ggcggtacct tcgatatttc gattctggaa
     5701 gtcggcgaca acgtcgtcga ggtcaagtca acgaacggcg acacgcacct tggcggcgac
     5761 aacatcgacc tgagggtcat cgagttcctg atcgaggagt tcaagaagga atccggcatc
     5821 gacgtcagca aggacaagat ggtgctgcag cgcctgaagg acgccgcgga aaaggccaag
     5881 atcgagcttt cgtcgatgat ggaaacggaa gtcagcctgc cgttccttac cgccgacgcc
     5941 agcggtcccc gtcacatgaa catccgcgtt tcccgcgcca agctcgagca gttgatgctt
     6001 gatctggtcg agcgttcgat tgaaccgtgc cgcaaggcgc ttgccgacgc cggactgaag
     6061 gcttccgaca tcgatgaagt cgtcctggtc ggcggttcca ccaggattcc gcttgttcag
     6121 cagagggtga aggaattctt cggccgcgag ccgaaccgca gcgtcaaccc ggacgaggtc
     6181 gtggccgtcg gggccgccat ccagggcgcc gtgcttacgg gcgaagtgaa ggacatgctg
     6241 ctgctggacg taaccccgct gagcctgggg atcgagaccc tgggcggcgt gatgaccagg
     6301 ctcatcgagc gcaacaccac gattccgacc aggaagcagg agacgttctc gaccgcggcc
     6361 gatggccaga caagcgtcga aatccacgtc cttcagggcg agcgcgagat ggccgccggg
     6421 aaccggacgc tgggcaagtt cattctggac gggattccgc ccgcgccgcg gggaattcca
     6481 caggtcgagg ttacttttga tatcgatgcg aacggcatcc tgaacgtttc ggcaaaggac
     6541 aaggcgacca gccgcgagca gaagatcacc gtcacggcat cttcaggcct gaccgaaggc
     6601 gacatcaagc ggatggttga cgacgcggcg aagaacgagg ccgaggacaa gcgtcgtcgc
     6661 gaggagatcg agacccggaa taagctggac acgatggtct atcagaccga gaagctgatc
     6721 aaggacaacc gcgagaagat tccggtcggt gaactggccg aggtcgagcg cgaaatcgag
     6781 aaggccaggg aagccctgaa gggcaacgat caggatgcaa tccggaaggc atttgacgat
     6841 gtcaccgcgg cgtcgcacaa gatggctgaa aagatgtacg cggccgcaag ttcgcagccc
     6901 ggtgccgccc ccggcgccga atcccagtcc ggcgcccccg aaggcgaagt catcgacgcc
     6961 gagattgaag agcagaaatg atcccatcca caaggtaagg gcgaatgaaa attcgcccgc
     7021 cgcggtcgag cacattccat ccgccccacg atccgtacgg gcgaatgaaa attcgcccgc
     7081 tgccttccct gtacgggcgg attacatccg cccgccgcgg tccagcaaaa tgccttccgt
     7141 tgcgccgcgt cccccgctcc gtaagggcga atgaaaattc gcccgcctgt ggggaaattc
     7201 gcccgtccta aatcacgtcg aagcttcagc gaaggtgtgt ggtcgttccg gggtaaatca
     7261 gttctgggtg gccggtgcgg cttcagcggc cggtgccggg gcaggggctt cggcggccgg
     7321 agcagcttct ggagccggtg cggcaccttc ggcggccgga gcagcttctg gagccggtgc
     7381 ggcaccttca gcagccgggg cgccttccgg ggcgggcggc ggcgccatgg ccttcatctt
     7441 ttccatctgg gcctgcatgg cttccttcat catgcgggcc atgtcgatcg cccagccctt
     7501 ttcggtccga accatcggtg cgctgttttc cttgccgttg gtcttataga cgacgcgcgc
     7561 ctcatccttg ttgtccgggc tcacggtctc ggaaatgact tccattccgc cggccttgct
     7621 gaagcccttg cacatctcct tgaagatgtc gaagtcttcg gcctggcctt ccggcttctg
     7681 ggcgaagttc ttgagctgct cggaaatctg ggtaagctgc tcgacgcttt ccgcggtgag
     7741 gccacccttg atgccggcga catcgcactt ttcgattgcg gtcatccatg ccttgaatga
     7801 atcaccgggg gtcgggaact tttccttgca ggcggtaaac gcgacaaggg acatggcaac
     7861 tgtcagcacc aacacgagct tcttcatctg ggactccatg aaagacaaaa aaatcaagga
     7921 tgtttcgtcc ccgaaacacc gcctggaaca ctgttttgat cgatgacttc gtcgatcagg
     7981 ccgtattccc tggcctcttc cggactcagg aacctttcgc ggtcggaatc cttcaggatt
     8041 tgttcaacgc ttcgtcccga atggttcgac agcagtttaa ccacctgatt cttcaaaaac
     8101 aatatttccc ttgtgcgtat atccacatcg gtcgcctggc ccgagaatga accgagcggc
     8161 tggtgcagca gtatccgcga atggggcagg gcccgacgct tgcctggcgt gccggcggca
     8221 aggagaaggg cgctcatgct ggaggcctgg ccgatgcaga tcgtcgaaat cgggcacctg
     8281 acgtactgca tcgtgtcata gaccgccagc gtggagttga cgttcccacc gggcgagttg
     8341 atgtaaagat agatgtcctt gtccgggtcc tctgactcaa ggtaaagaag ctgggcgatg
     8401 acaacgcttg ccaggttgtc gtcgatttcc cagccaagga agatgatcct gtccttcagg
     8461 agccgggaaa atacggtcca ttcacgttcg ccgcgggccg tatgctcgat taccgtggga
     8521 acccaccaca ttggatactc cttgcgttgc gtcgccgtgg cacgccttcc ggcgtcagtc
     8581 ctgcttcggg gcggcagcct ttttcgccgc gggcttcctt gccggcttct tcgccgcctc
     8641 gggcgccgca tcctcggccg gtgccgcggt ttcttcaacc ggatcctgct tttcggcggt
     8701 cttcttcgtc ctggtcgcct tcctggccgg cttgtcctcg gcagtggggg ccgcggcggc
     8761 ctgaaccgcg ccctcggcac ccggggtcac aacggcatgc ctggtcagca gatccagggt
     8821 cttgccgacc cgaagctgcc tgccaagctg ttcaagcctt tcctggccga aaatcgcctt
     8881 gatcttcggc agcggcattc cataggcgtc ggccgtttcc tgcagcttgg cctcgatgtc
     8941 cgcttccgtc acctggattc cttccttgtc tgcaatcgct tcaaggatga ttgaccgggt
     9001 caggttgcgg cgggcttcct cgagagactt ctcccgcgga acgccaaggt cgaaatcctt
     9061 tgcgagttcg gcgcgttttt cttccggaat ctgctgaaga accctgtcac gggcttcctt
     9121 ttccgccatg tcggccaggc gctcaatggt ccgttcgccg atttcaaagg ggttgttttc
     9181 acagatcctg gacaggatga gttcttcggt ctcttccttc agctttgaat cccgctgctc
     9241 ttcaagctgc ttcttcagca gggcatggaa ttcctcgaca gtctcaacat ccgcatgctt
     9301 cttgacgaat tcgttgtcca gttcgggcat cttccgggcg cggatctttt cgatcttcac
     9361 aaccgcgcgc acgtccccgg cctgatcggg atccatcttc tgcccgcggg cggtgattcg
     9421 gccaatcctt ccctggacgg tcttttcctc gccggcggcc aggccaagaa tctggcgtcc
     9481 gataccttcg acaggttcgt cgtccgtccc gcccatgatc ctggacgagt gggcatcctt
     9541 gataaccgtg ccgtcggaaa ccagcgacag ggtcacctgg gtttcggcca tgtcgccttc
     9601 ctcgacgacg caaccttcac ccttgtcctc gaagcgggac agggatttgc gaagaccgtc
     9661 gatgcgttcc atgatctggg cttcggtcac ttcgggagcg aacggccccg ccacttcgac
     9721 ttccaggtag tccttcacat cgacttccgg gaagacctcg atttcagcgg tgtattcgag
     9781 tgcgttctct tcgtcgcgca caaggtcgaa cttcggttcg gtcgatgcct tcagcttgaa
     9841 ctggtcaatg gcgtccccga acgtctgtga gatcaggttc tcgcgcactt cgtattcaat
     9901 gcgcggacca agctgggcca gcacaaggtg catcggcgcc atgccgggcc tgaaaccggg
     9961 aacgcggacc tgggcggcgt acttgcgggc cagcctgcgc ctttccttgt caaacccttc
    10021 cttgtcgatg gcgacggtga tgcggtgtgt aagctttcca agatcttcgt gatcaacttt
    10081 catcaaatga ctccttgcag cggcgaaccg tgtcagattc caaaaggcgc ggggaatgta
    10141 tcagttttac ctgaccttgg caaggcggcc cgcgggcctg aaccacggga acaactggcc
    10201 ggccaggaag ccgagtccgt gccggatcca gagcggatta tccttaagga cgaaccatgc
    10261 cgtgggaaac aggatgcggg gatccaggta gaatcttcgg tatgcccagg cgccaagcct
    10321 gttgatttcc cggttggaaa gtccgcacat gtcaccgtcg atgccaaact gttcaaacag
    10381 ctcgctggga tccagccgca tcagcggata aaaggccgcg tactgggggc gaaccttcaa
    10441 tgcgaacctg acgtcggcct caagcgtggc gcggttccac cccggaaggc cacatatgaa
    10501 ttcgacgacg gtctggagcc cgatcttcct ggccgccgcc acgttggcgg cgacgatttc
    10561 cggttcccgc ggggatcttc ggatggcttt aagaatgtcc ggatcggttg actgcaggcc
    10621 gaagataagc aaatcgcacc cggccctgcg gaagcgttca aggcgctgcc cgcgatcctt
    10681 ttcgaatacc gaagggtggg tctggcacgt ccatttcagg tcgtatccgc gttcaatcaa
    10741 tccttcgcag aattcccttt cccagtcggg atcgaccgcg aagtaatcgt ccttgaaggt
    10801 gatgtacctg atgccgaaat cgcgcaccag ttcgtcgatt tcgcaaagca cgttcgcggc
    10861 cgagcgaagg cggaccgtcc tgccggaaac gaaaggaacg ctgcagaagg tgcaacgcct
    10921 gtggcagccg cgggaagtga tcatggtcgt gtacggcttg cgcatcccga gtacgcgcca
    10981 gtcgtggtac ctgtcgatgt cgatgatgtc ccttcgtgga aatggaatcg agtcaagatc
    11041 ggctatcggc tgccgcgcag ggtttataac gatgcttccg tcaaccgaaa aggcgatgcc
    11101 gtcgatttcc cggggatcca gttcgccgtc gattgcccgg atcatctcga cgattgcttc
    11161 ctcgccttcg cctgtacaga ccatcgtggc cccggcgttc aagtaaggcc cgggccggta
    11221 ggcgtccgcg ccgccaacca gtatcggcag ttccggcgcg gccgcccgca gttttacaat
    11281 ccagtggcag accgcgtcgc gcgtgattga atccgaatag aatccaacga agacaagacc
    11341 ctcgttgcgg gccttcgcgg ccagttcgtc cggtccgaag cggtcaaggg ttcggtccat
    11401 gacctggcag tcgattccgt tttccttcag caccgcggca aggtaaagaa atcccaggtg
    11461 cggaaccttc ttcatcgaca gttcggcgtg gcgggcggcg gccaggtact cggcctgggc
    11521 gctggtatat acgaaaaggg ccctgcgcgc gccatgattg gttccgagag tcatgcagcc
    11581 tccggtcggg ctgccagttt cttaccatcc cgccgcctgt gtcaagcgcg gctgaagcga
    11641 atgcgtttcc acgcggtccg ggcgaacctg atgtgaaggc cgtcgtcatc catccgttgg
    11701 aatgatggta tgctttgtgt cggcttgcgg ccgggccgcg ggcatctgca tgttttccgg
    11761 gggatctgcg atgaatgatt cg
//
LOCUS       DAOPPA010000032        11754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6304_length_11754_cov_4.268570, whole genome shotgun sequence.
ACCESSION   DAOPPA010000032 DAOPPA010000000
VERSION     DAOPPA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11754
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6304_length_11754_cov_4.268570"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..902)
                     /locus_tag="PKH54_01875"
     CDS             complement(231..902)
                     /locus_tag="PKH54_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC98664.1"
                     /translation="MCLIPAGSFWMGCNESVDTQCGSDEKPYHEVMLSGYYIDRTEVT
                     QSEFKKCVDAGECGTPDCGWDPTGDPDKPVVCVDWNDATAYCTWAGKRLPTEAEWEKA
                     ARGTDGRKYPWGNETATCDYAVMSVCPGDAMDVCSKSPAGDSPYGLCDMAGNVWEWVS
                     DWYDSGYYTYSPALNPTGPVSGSDRVLRGGGFYYYYNLRASYRYYYYPSYFSDGLGFR
                     CAMPQ"
     gene            complement(1047..3002)
                     /locus_tag="PKH54_01880"
     CDS             complement(1047..3002)
                     /locus_tag="PKH54_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC98665.1"
                     /translation="MNLKLTCLAAVLVAVLFCPAAMSQEKDKIIFVSDLRDFSKNVSP
                     EVLDLFTEIVRDGARRYATGYSLMDKENAQVILEGFGIDLQDCLMAGRDDCEVKFAER
                     VQADIGVSGQILQLPEGGIRLVLKMHDVKKRSPINSTIKGAADINELERVLTESVVEL
                     YAPLYRPDSGGGVIGGGRETGGRRIDRGEKIVNKKTDTKGLVFIETNPPGADIQINGK
                     PVGTSPYQASLMVGRYVVIAELNSYYHVARHEFELTSDGSTIELVLPPAFGEIEVSST
                     PSGAEVFVDDKPVGKTPYRDSQLLSGDHRIEVRKSMFKPIRRNVVVKDGQSIRESFAL
                     VEDFGGIKISSEPSGAVITLDGKDTGKRTPALLRPVKEGTHILNLDLPGYGRVGDTVN
                     VIAGRVIDVHKSLEAKYGTLAVMTRTADGTPCRGNLKVDGEAKGKTPWKGQLLAVEHE
                     ISVECGARPTVERRTIEHNTSSEITITVAGGPPLRPVAVKRRKDNASSVGSVADKVSS
                     AGAGADKMDLRRKRASFQARLSGGLDLYRASQGVGGLVQVGVGTGRWFDIHTGVGFPS
                     YSWVTDFEVNLWPYGRFVPTLAARVGLGFHPDHNVYSIDFTAGVEMWMANWAAFFVKI
                     GVGYSWNRTFDVNGVYVPGWLGLEFRY"
     gene            complement(3061..3756)
                     /locus_tag="PKH54_01885"
     CDS             complement(3061..3756)
                     /locus_tag="PKH54_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC98666.1"
                     /translation="MVDGFRYPARFATLVLTVCVALFPLVSTAADQAAPAEAVVPSEG
                     QPVPKFGAPDLDRNVFTVARMLSELAADNGCAALVFFTTVCEPCRREILQLKSESARL
                     SSAGVRVVLVAAGEDRPKVRSFQDRIQVPFRTVTDVNMLISRLFGLTTPQMTVTVPRT
                     FVFGADGVVKAVIAEPGTDVVNDIISACRGSSSARPPASLPPSSLPAVPAPPPSSPPV
                     PVNPTVPATHSGN"
     gene            complement(3929..4774)
                     /locus_tag="PKH54_01890"
     CDS             complement(3929..4774)
                     /locus_tag="PKH54_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98667.1"
                     /translation="MTGKPATAIQYDCAHTARVKSALLFIATRLGDMMDDIIVVGGLV
                     PTLIINQSNSMPGVEPHAGTMDLDLGLKLAVLESGRYREISARLRDAGFRPDRNERGH
                     QTNHRWVVSGTPEISVDFLISPSCSSDVPGRLKNIESDFAAVVASGLECAFHDRMSVN
                     IRCHTIQHEAASRNLWACGPGGFIVMKALAFEGRGENKDAYDLNYVLRNFAAGINDVA
                     SHLHRILDHHDARKAISILERDFCRHDAPGPVRAALFLTGKADEVVCAEVSTNVRTLL
                     ALLKK"
     gene            complement(4764..5696)
                     /locus_tag="PKH54_01895"
     CDS             complement(4764..5696)
                     /locus_tag="PKH54_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98668.1"
                     /translation="MTFEFGSRFVVEFRKSATSAPVLSAVEKVRAMAARFDGIVPLVA
                     VPYMGEAGRKICHERGVGWFDLSGNAFIVAPGLQVVIEGKRDAFVSSGRPTNLFAPRS
                     SRIVRMLLANPLEQFSQARISELTGLDRGFVSRLLSRLVAEELVSRQKDRTVRVNDRG
                     LLLDAWRDSYNFDRHHCIRGSLPARSGEALLHQVASSLTGEGLEYAATGLGAAWLMTG
                     FAAFRTVTIFVREEIPSEFLERIGFISESRGANLWLVVPDDDGVFQWTRPKDGVICAH
                     PAQVFVDLKGHHERSADAAAMLRRQILDWNKDDR"
     gene            5998..8820
                     /locus_tag="PKH54_01900"
     CDS             5998..8820
                     /locus_tag="PKH54_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC98669.1"
                     /translation="MRLLNPSALLVSVMLAAAALAGCGGGPVASDVPGDVGQTGDDHT
                     VPSFASLADFLAYAGEATPAQVKFVIRDFQGPAVVHFEEPGFYAMHDEWSWFSLLNGI
                     EIPGYDFAPINGMSFPTVGAIFDWARGRSPLPLDLVFVAEGTRLYSPKFYDRAFNRTS
                     SQGTLAPRFFGCGSVLYFPPDDRRPIPVETWGFELEYGDVPTETDVERFFESIGEALP
                     EGVAGEIIWIVRSETQKKVAQTIDAGGGPLAGRWATWDMLQVPGESQTYNPGISAGFV
                     KIFEPGEVPLVDAARTVAVLREIPDDLPPVAAIVTNVPQTPLSHIGILAKARGTPNLY
                     VAGIADDPRFVTWASYKRPIIVKATADGHEFREMSNAEWSQYMSLQAPRQADIQPVDV
                     SGFPDTFDPGAATPTEMEGLIPMIGGKTAGFIMLNSIGELDKPDAPIGITVRGYREFI
                     DTLDPPLDQILAHSAMTDSRVVLAVTEGPNAYLAANGGSDAAQKWLDAFRTAYLGTVI
                     GRAVEAGGIRQMVMTAPMDAGFVARITSALEERFNFLDDAQGLRFRSSSSAEDVEGFN
                     GAGLYESHTGYLHPELQSPKKRDKTVEAALRRVWASYWLNNAFQERALAGIDHLAANM
                     GVLVHPVFDDDREAANGVLTLELVRRAGGDVVTLTANMQDGALSVTNPPPDSNALPEV
                     DVVTRTGADDPVISRVRASTEVAPGTLLLSDTELTSLLDSFVKMASDWLDLRNKDLLS
                     ARKRSTLTVDAEIKRMSPGWPSLKDGTVNPARLVLKQARTVERPVLAGDAIRKLPVPD
                     DLLSYAILARQRTCQCTAFRVDVFEFTTDPAHPWPFDYSKTPFVSHAALRFTTTIPDF
                     DFNPGDLRMLTHLHATFTFDDDRQWRMTVTPIDTQAAGFASLELDQAGEWTMTGGGGQ
                     SADGADLACTATTILTSPLAFLDSLM"
     gene            complement(8914..9770)
                     /locus_tag="PKH54_01905"
                     /pseudo
     CDS             complement(8914..9770)
                     /locus_tag="PKH54_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9967..11655)
                     /locus_tag="PKH54_01910"
     CDS             complement(9967..11655)
                     /locus_tag="PKH54_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98670.1"
                     /translation="MKIFHCFRKGLGCGGGCPIRAGLAAFPLRIDRYAIVLVFLAQVL
                     FFPYFPKMNSANELSRLYLALAMVENRNFEITPFISRFGNINDKSVIRDTVTVDGKAH
                     RSERFYSDKPPGTALVAAPAIALRHLVAGFTCLRGDMRLARLFVCILPTLLLLGLMRR
                     EMEELGVGSPVRAIAILGYGLGTLAMPYSVTYLAHQPVAVMLYAIWFLLRRRPEPPSL
                     KAAAGAGFLAGFCLMTEYQAAVYLLPLAVLFLVRVRPLARGLPVAIAAAAIPLAFLAF
                     YHHQSFGGVLQTGYNHIANPFFASVHQKGFMGVLYPGWTQFAGSFFLPSKGLFVFSPF
                     LLLGFVGIVSYFRVAGRIDGAFRLAQILLPALFVSSMVYWDGGWTVGQRHLTPMIPFL
                     IAPAALLMQRSLAAGIAAPGLVGASIMYTGLATVVYPHLPENIPNPFHDLTIPLFKGG
                     CLAQVFVLTELPSYAVLWVLAAVIFLALALAAVLRFVSSSEMPRPGARRIAAAVAAVI
                     LLAAIPAGWYGWTSQVARLDSAAAAKHRVYFEDQCRAAGRWNDVDPDAGQTCGE"
BASE COUNT         2577 a         3577 c         3347 g         2253 t
ORIGIN      
        1 aatacaattc ctggccatcg cagacgaata ttcattcgcg cctgactaca aggaaagctc
       61 atctacagac tccagaaatc gggtcgccgg cgtgttgcgg ccccgatgcg ctgtcggctg
      121 cgctcgacaa cgcgcatgtg accgtacccc gcctgcgacc tggccacatc cgaatttttg
      181 cggctttcgc cgcggatcag caaaacagtg gaacagtaat ccagtttttc tcattgcggc
      241 atggcacaac ggaagccgag gccgtcgctg aagtacgacg ggtagtagta gtagcgatag
      301 gacgcgcgga ggttgtagta gtagtagaag ccaccgccgc gtaagacccg gtccgagccg
      361 ctaaccggac cggttggatt taatgctgga ctatatgtgt aatatcccga atcgtaccag
      421 tcggaaaccc actcccaaac gttacccgcc atgtcacaca atccgtaggg gctgtcacca
      481 gccggcgatt tgctgcacac gtccattgca tcacccggac agacagacat caccgcgtaa
      541 tcacaagtcg ccgtttcatt gccccacggg tacttccgac cgtcagtccc tcgggctgcc
      601 ttctcccatt ccgcttcggt cggcaatcgc ttcccggccc atgtgcaata agcagtcgcg
      661 tcattccagt caacgcaaac cactggcttg tcaggatcac ccgttggatc ccagccgcaa
      721 tctggtgtcc cgcattcacc agcgtcaaca cacttcttga actccgactg cgtcacttca
      781 gtcctgtcaa tgtaataccc ggacaacata acttcgtgat agggcttttc gtctgaacca
      841 cattgtgtat ccacagactc attacagccc atccagaacg atcctgctgg aatcagacac
      901 atgccgttac tgcaattcac cgtgtaatca tctggttgcc agtccacaaa atccccgggc
      961 caggcacatc cagcgacgga cagaacggcc agcgcaacaa ccgccagaaa aatcggtctt
     1021 gtaatccagt tcatcccgcc accatcctag tacctgaatt ccaggccgag ccagccgggg
     1081 acatagacac cgttgacatc aaatgtccgg ttccaggagt aaccaacgcc aatctttacg
     1141 aagaacgcgg cccagtttgc catccacatt tcaacgccgg ccgtgaaatc gatggaatac
     1201 acgttgtgat caggatgaaa gccaaggccg acgcgcgcgg ccagagtcgg cacgaaacgg
     1261 ccgtatggcc acaggttgac ttcaaagtcg gtcacccagg aatacgatgg gaacccgacg
     1321 ccggtgtgta tatcgaacca tcttccggtg ccgacaccga cctgaaccag cccgccgaca
     1381 ccctgtgacg cacgatacag atccaggccg ccggaaagcc gcgcctggaa cgacgcgcgc
     1441 ttacggcgca gatccatctt gtccgcgccc gcgccggcgg aagatacctt atcagcgaca
     1501 gaaccaactg aagatgcgtt gtccttgcgg cgtttaaccg caaccggccg aaggggcggg
     1561 ccaccggcca cggttatcgt tatctcggaa ctggtgttat gttcaatggt ccttcgttca
     1621 accgtcgggc gggcgccaca ttcgaccgat atttcatgtt caacagcaag aagctgacct
     1681 ttccatgggg tcttaccctt cgcttcgcca tcgactttaa gatttccccg gcatggcgtc
     1741 ccatcggccg tccgggtcat gacggcaaga gtcccatact tggcttccag ggacttgtgg
     1801 acatcaatca cgcgaccggc aatcacattg accgtgtcgc cgacccgccc gtatccaggc
     1861 aaatccaggt tcagaatgtg cgtgccttcc ttgaccggcc ttaacagggc cggcgtcctt
     1921 ttgccggtat cctttccatc aagcgtgatg accgcaccgg acggttccga gcttatcttg
     1981 atgccgccga aatcctcgac aagcgcaaac gattcacgaa tggactggcc gtccttcacg
     2041 accacgttcc gccgaatcgg cttgaacatg gatttgcgaa cctcgattct gtggtcgcct
     2101 gaaagaagct gggaatcacg ataaggggtc tttccgaccg gtttgtcatc gacaaacacc
     2161 tcggcgccgg atggcgtgct tgaaacttcg atttcaccga aagccggcgg cagtaccagt
     2221 tcgatcgtgg aaccgtccga ggtcagttcg aactcgtggc gggcgacgtg ataatacgag
     2281 ttcagttccg caatgacgac gtaccggccg accatcaggg aagcctggta aggcgaggtt
     2341 ccgaccggct taccgttgat ctgtatgtcg gcacccggcg gattcgtttc gatgaagacc
     2401 aggcctttgg tgtcggtctt tttatttacg atcttttcac cgcgatcgat tcgacgccca
     2461 ccggtttccc ttccgccacc gatgacgcca ccgcccgaat cgggccgata aagcggggcg
     2521 tacagttcga cgaccgactc ggtcagcaca cgttcaagtt cattgatgtc cgccgcgccc
     2581 ttgatcgttg aatttatcgg ggatcttttc ttgacgtcgt gcatcttcag gaccaggcgg
     2641 atgccgcctt ccggcaattg aaggatctgg ccgctgacgc cgatatcggc ctggacgcgt
     2701 tcggcgaact tgacctcgca gtcgtcgcgg cccgccatca ggcagtcctg caggtcgatt
     2761 ccgaaacctt ccaggattac ctgggcattt tccttgtcca tcagggaata tccggtggcg
     2821 taccgacggg cgccgtcacg gacgatttcg gtaaaaagat ccagaacttc ggggcttaca
     2881 tttttcgaaa aatcacgaag atccgaaaca aagatgatct tgtccttttc ctgcgacatg
     2941 gcggccgggc agaacagcac ggcaaccagg acggcggcca ggcatgtcag cttcaaattc
     3001 atcgaacacc acttgacggt ttgcgactgc accgaacgat acctgaaagg gtttacacgg
     3061 tcaattcccc gaatgggttg caggaaccgt cggattgacc ggaacgggcg gcgatgacgg
     3121 cggaggggcg ggaacggccg gcagggatga gggcggcaag gatgccggcg gcctggcgga
     3181 agacgaacca cgacaggccg aaatgatgtc attaacaaca tcggtgcccg gttccgcgat
     3241 aacggcctta acaaccccgt cggccccaaa aacgaacgtc ctgggaaccg tcaccgtcat
     3301 ctgcggggtg gtcaagccga acagcctgga aatgagcata ttgacgtccg tgaccgtccg
     3361 gaacggcacc tgaatccgat cctggaacga ccggactttg ggcctgtctt caccggccgc
     3421 gaccagcacc acgcgaacac cggccgacga aaggcgggcc gattcggact tcagttgaag
     3481 gatttccctt ctgcacggtt cgcagaccgt cgtgaaaaag accagcgccg cacagccgtt
     3541 gtccgcggca agttccgaca gcatccttgc gaccgtgaag acgttcctgt ccagatccgg
     3601 ggccccgaat ttcgggaccg gctggccctc cgatgggaca accgcctcgg caggggccgc
     3661 ctggtcggcc gcagtcgaaa caagcggaaa caaggccacg cagaccgtca acaccagtgt
     3721 cgcgaatcgc gccggatatc gaaatccatc aaccaacagg caccccggac aatgcgaacg
     3781 gatcaccaaa taaatccacc cgttgcgaca gtcaagcgca acgacgccca gtatagtcga
     3841 aaagcccggg gaactttgta caatctgccg gctcgagtca ttgggaagcg aaggaacgca
     3901 acaggaatca cagaccgtcc cgggaccatc atttcttcaa caatgcaagc agtgtccgga
     3961 catttgtgga aacctcggcg caaacaactt catctgcttt tccagtcaaa aacagggccg
     4021 cacgaaccgg ccctggcgca tcgtgacggc agaagtccct ttccagaatc gatatggctt
     4081 tcctggcgtc atgatgatcc aggattcggt gaagatgact ggcaacgtcg ttaattccgg
     4141 cggcaaaatt ccttaacacg tagttcaaat cgtatgcgtc cttgttttcg ccacgtccct
     4201 caaatgcaag cgccttcatc acgatgaatc cgccggggcc gcatgcccac agatttcggg
     4261 atgcggcttc atgctggatg gtgtggcacc gaatattgac actcatgcgg tcatgaaaag
     4321 cgcattcgag accggaagcg accaccgccg caaaatcgct ttcgatgttt ttcaaccgac
     4381 caggcacgtc actcgaacaa gacggcgaaa tcaggaagtc cacactgatt tcgggagtgc
     4441 cggatacaac ccaccgatga tttgtttgat gtccccgttc atttctatcc ggacggaagc
     4501 cggcatctct aagccgggca gagatctccc gataccttcc ggattccagt acggccagtt
     4561 tcaggcccag atccagatcc attgtccctg cgtgaggctc aacgcccggc attgagttgc
     4621 tttgattaat aatcagggtc ggtaccaggc caccgaccac aataatgtcg tccatcatgt
     4681 cgccgagccg cgtcgcaata aacagcagcg cggatttcac acgtgcggta tgagcgcagt
     4741 cgtattggat tgccgtggcg ggcttaccgg tcatccttat tccagtccag gatttgccgt
     4801 cgcagcattg cggcagcatc ggccgatctt tcgtggtgtc ccttcagatc gacgaagacc
     4861 tgtgccggat gggcgcaaat gacgccatcc ttcggccgcg tccactgaaa gacaccatcg
     4921 tcatcgggta caaccaacca caggttggct ccccttgatt cggaaataaa gccgatccgt
     4981 tccaggaatt cggaaggaat ttcttcacgc acaaaaattg tgactgtccg gaaggcggcg
     5041 aagccggtca tcagccatgc ggcaccaaga ccggtcgcgg cgtattccag accttcgcca
     5101 gtcaggctcg atgcaacttg atgaagcagc gcttcgcccg accgggccgg caggcttccc
     5161 ctgatacaat ggtgacggtc gaagttatat gagtcgcgcc atgcatcaag caacaaacca
     5221 cggtcattca cgcgaaccgt tcggtccttc tgcctggata caagctcttc ggcaacaagg
     5281 cgggacaaca ggcggctgac aaaaccgcgg tcaagaccgg taagttcgga aatcctggcc
     5341 tgactgaatt gttccaatgg atttgccaac agcatccgga cgattcttga gcttctgggg
     5401 gcaaacaagt tcgttggacg cccggatgaa acgaaagcat cgcgcttgcc ttcaatcaca
     5461 acctgcagac cgggagccac gataaaggcg ttgccactta gatcgaacca acccacgccg
     5521 cgttcgtgac agatttttcg tccggcttcc cccatgtaag gaaccgccac cagggggaca
     5581 atcccgtcaa accgggcggc catggcacga accttttcga ccgcggaaag cactggggct
     5641 gaagttgccg acttgcgaaa ctcgacaaca aacctggatc caaattcgaa tgtcaaatcc
     5701 gccccttgaa cccagccggc ctggccggat tccaatactc tggccttctg attaaaaggc
     5761 gcaaaaatcc ggttcaagac cgccggcatt gccgccacaa gacgcctgtg ttcgtttgat
     5821 tgcatcattg ataaacaaat acccttcatt gctgaatctg tcaatgtagc caatttatgc
     5881 aaatcaccct gacaaatcaa ctgcgacgtg aatttcgcca acgcttgaac tttcactctg
     5941 acttcacaaa acacgtatca tccggaatcg tgacgacagc ttttccgggg aggcgcgatg
     6001 agactcctga acccgtcagc attattggtt tcggtgatgc tggccgcggc ggcacttgcc
     6061 gggtgcgggg gcgggccggt ggcgtccgat gtgccgggcg acgtgggcca gacgggggac
     6121 gaccacacgg tgccatcgtt tgcaagcctg gcggacttcc tggcgtacgc cggggaagcg
     6181 acgccggccc aggtgaagtt cgtcatccgg gacttccagg gcccggcggt ggtccacttc
     6241 gaggaacccg gcttttatgc gatgcacgac gaatggtcat ggttttcgct tcttaacggg
     6301 atcgagatcc cgggatacga cttcgccccg ataaatggaa tgtcgtttcc gaccgtgggc
     6361 gccatttttg actgggccag gggccgaagt ccgcttccgc ttgacctggt gttcgtggcc
     6421 gagggcaccc gcctttattc gccgaaattt tatgaccgcg cgttcaaccg taccagcagc
     6481 cagggaacgc ttgcgccgcg atttttcgga tgcggatcgg tcctgtattt cccgcccgat
     6541 gacagacgtc cgattcccgt tgagacgtgg ggtttcgagc ttgaatacgg cgacgtgccg
     6601 accgaaaccg acgtcgaaag gttctttgaa tccatcggcg aggcgcttcc cgaaggcgtt
     6661 gccggcgaga tcatctggat tgtaaggtcg gaaacgcaga aaaaggtggc ccagacgatt
     6721 gacgccgggg gcgggccgct tgccggcaga tgggcaacct gggacatgtt gcaggtgccc
     6781 ggcgagtccc agacttacaa cccgggcatc tccgccggct tcgtgaagat ttttgaaccg
     6841 ggcgaagtgc ccctggtcga tgcggcaagg accgtggcgg tgctgcgcga aatcccggac
     6901 gaccttcccc cggtcgccgc gatcgtcacg aacgtgcccc agaccccgtt gtcgcacatc
     6961 ggcatcctgg caaaggcgcg cggcaccccg aacctgtacg tggcgggaat cgccgacgac
     7021 ccgcggttcg tgacgtgggc gtcgtacaag cggcccatca tcgtcaaggc gactgccgac
     7081 gggcacgaat tccgggaaat gagcaacgcg gaatggtccc agtacatgtc gcttcaggca
     7141 ccgcgccagg ccgacatcca gccggtcgat gtgtcgggat tcccggacac cttcgatccg
     7201 ggcgccgcga cgccgacgga gatggagggc ctgattccga tgatcggcgg caagacggcc
     7261 gggttcatca tgttgaactc gatcggtgaa ctggacaaac cggacgcgcc gattgggata
     7321 accgttcgtg gttaccggga attcatcgac acactggatc cgccgcttga ccagatactg
     7381 gcccactccg cgatgaccga ctccagggtc gtgcttgcgg tgaccgaagg gccgaacgcc
     7441 tacctggccg ccaatggtgg cagcgatgcg gcgcagaagt ggcttgacgc gttccggacg
     7501 gcttacctgg gaacggtcat cggccgggcc gtcgaggctg gcggcatcag gcagatggtc
     7561 atgacggccc cgatggacgc gggcttcgtc gcgcgaatca cgtccgcgct ggaagaaaga
     7621 ttcaacttcc tggacgacgc ccaaggactg cggtttcggt cgtcgtcgtc ggccgaggac
     7681 gttgagggct tcaacggcgc gggcctttat gaatcccaca ccggatacct gcacccggaa
     7741 ctgcaatcgc cgaaaaaaag ggacaagacg gttgaagccg ccctgcgccg agtctgggcg
     7801 tcatactggc ttaacaacgc attccaggaa cgggccctgg ccgggatcga ccatctggcc
     7861 gcgaacatgg gggtgctggt gcacccggtg ttcgacgacg accgcgaggc ggcaaacggc
     7921 gtcttgacgc ttgagcttgt gcgacgggca ggtggcgacg tcgtgaccct gaccgcgaac
     7981 atgcaggacg gcgccctgtc ggtgacgaat cccccgcccg actcgaatgc gctgcccgaa
     8041 gtcgatgtcg ttaccaggac cggcgcggat gacccggtga tttcgcgcgt ccgggcctcg
     8101 accgaagtgg ccccgggaac cctgctgctt tccgacaccg agctgacgtc gctgcttgac
     8161 tcgttcgtga agatggcgtc tgactggctt gacctgcgaa acaaggatct cctgtcggca
     8221 aggaagcgtt ccacgttgac tgttgatgcg gaaatcaaac ggatgtcgcc gggatggccg
     8281 tcgctgaagg acggcacagt gaacccggcc aggctggtgc tgaagcaggc tcgaaccgtc
     8341 gaaaggccgg tgctggccgg tgacgcaatc aggaagctgc ctgtgccgga cgacctgctg
     8401 tcgtacgcga tcctggccag gcagcggact tgccagtgca cggccttccg cgtggatgta
     8461 tttgaattca cgacggatcc ggcacatccc tggccgtttg actactcaaa gacgccgttc
     8521 gtttcgcacg cggccctgcg atttacgacg acgattccgg actttgattt caaccccggc
     8581 gacctgcgga tgctgaccca ccttcacgcc acgttcacgt tcgatgacga ccgccagtgg
     8641 cgcatgaccg tgacgccaat tgacacgcag gcggccggat tcgcgtcgtt ggaacttgac
     8701 caggcgggcg aatggacgat gaccggtggc ggcggacagt cggccgacgg tgccgacctt
     8761 gcctgcactg cgacaacgat tcttacttca ccgctggcgt tcctggattc gctgatgtag
     8821 ggggctgccc gggacgggcg aattttcatt cgcccttacg gaatccggac ggatgcgtgc
     8881 ggcccgtaag ctccacacct ttcgaacaaa aactcactcg tcggaagttt ccttcaattg
     8941 ctttgcggat ctgtcggtcg gcagaaaact ttcgccactc accacgcttc tgccagtctt
     9001 ctgctccaat tcccggcggg cccgcctggc aattccgcca cccttcctgc cagcgctggc
     9061 gttttccacc atgccggtgg cctgtacgct ttcggcaatc tgccgcgtag aaagttccgc
     9121 aagcgccgtg aagataagtt ccgcctcggt catatggtcg cgcaagttct gtacatccaa
     9181 tcctttgatc ttcttatgtt ccttgactga aacccccgcc attcctgatg gatgatgttc
     9241 gtcaatatgg catattcgtc agcgccactg acgtcatggc ttttccaata atccgtcagc
     9301 ttgttcctgg tttcctggcc catcatccgc tgctggatcc atttttcgct tcggccatgc
     9361 tggcgccagt aatcacgggc gcgatccagc gaccgcgccg ggtcgcccat gtcctgaatt
     9421 cgctcgtaac caacccttgc aagccaaagc ttaatcggtt cagctttggg actgggaacg
     9481 gactgaacta ttcgcaaaag cacttccgga tcggcgacat ccgtcagata ggattttcca
     9541 tcgctggccg tcaacttcag tcggttacat tttgtaaccg actcgctacc ttccttcttg
     9601 agcctgtttt tcaacacctt ccagtagtta cgcgcggcct gatagtccgg ctgatggatc
     9661 agcgcggcta caatgtcaac gacagaaaag aaccaggtct cggaatcagc gtcgtacacc
     9721 cgacgaatct tgaagtcctc gaagaccgcc agaacctgtt ccttactcat cgtcctgccc
     9781 tccaattacg cttgcccggc tatggccaga acacagggat atgaaaaact agcaccatcg
     9841 acagtgaggc gccacaccaa tcctcgccga acaaatattc ggggaaatac ggcgaaggct
     9901 atcccgggaa gtgcaacggc attgaccgtt catgatacgt tgactggaac cgcagaatcc
     9961 ggggaatcat tcgccgcagg tctggccggc gtcggggtca acgtcgttcc agcggccggc
    10021 tgcccggcac tgatcctcga aatacacccg atgcttcgcg gccgcggccg aatcaaggcg
    10081 cgcgacctgg gacgtccatc cataccagcc ggccggaatc gccgccagca ggatgaccgc
    10141 agcaaccgcc gcggcaatgc ggcgggcgcc cggccgtggc atttcggacg acgaaacgaa
    10201 acgcagaacc gcagccaggg caagcgccag gaagatgacg gccgcaagca cccacagcac
    10261 cgcatacgac ggaagttcgg tcaggacgaa tacctgggcc aggcagccgc ccttaaaaag
    10321 cggaatcgtc agatcgtgga acgggttcgg gatgttttcc ggcaggtgcg gatacacgac
    10381 tgtcgccagt ccggtgtaca tgatggacgc gccgacaagg ccgggcgcgg cgattccggc
    10441 ggccagcgaa cgctgcatca gcagggccgc tggcgcgatc aggaacggga tcatcggggt
    10501 caggtgccgc tggccgactg tccatccgcc atcccaatag accatcgagg aaacgaaaag
    10561 ggccggcaga aggatctggg caaggcggaa cgcgccgtcg atgcgccccg cgacccggaa
    10621 atagctgacg atgccgacga atccaagcaa aaggaacggc gagaacacga acaggccctt
    10681 tgacggcagg aagaaactgc cggcgaactg ggtccagccc ggataaagca cgcccatgaa
    10741 gcccttctgg tgaaccgacg cgaagaacgg attggcgatg tggttgtaac cggtctgcag
    10801 gacgccaccg aacgactggt ggtggtaaaa cgccaggaac gccagcggga tcgcggccgc
    10861 cgcaatcgca accggcagcc ccctggccag cggccgcacg cgcaccagga acagaaccgc
    10921 cagcggaagc agatagacgg ccgcctggta ttcggtcatc aggcagaacc cggccaggaa
    10981 cccggccccg gcggcagcct tcaggctcgg gggttcggga cggcgccgca acaggaacca
    11041 tatcgcgtaa agcatcacgg cgaccggctg gtgggccaga tatgtgaccg aatacggcat
    11101 cgcaagcgtt ccaaggccgt aaccaaggat ggcgatcgcc cggacgggcg acccgacccc
    11161 aagttcctcc atctcgcggc gcatcagccc aagaagcagc agtgtcggaa ggatgcagac
    11221 aaacaggcgg gccaggcgca tgtcgccacg caggcatgtg aacccggcca caaggtggcg
    11281 cagggcaatc gccggggcag ccaccagagc ggttcccggc ggcttgtcgg aatagaagcg
    11341 ttccgaccgg tgggcctttc catcaacggt gaccgtgtca cgaatcaccg acttgtcgtt
    11401 gatgttgccg aaccgggaaa tgaatggcgt tatctcgaaa ttccggtttt cgaccatggc
    11461 cagggccaga tacaaacgac tgagttcgtt ggccgagttc atcttcggga agtacgggaa
    11521 gaacaggacc tgggccagaa agaccaggac gatggcgtaa cgatcaatgc gcaggggaaa
    11581 tgccgccaac cccgccctga tcggacatcc gccgccgcac cccagtcctt tccggaagca
    11641 atggaaaatc ttcattgcgc gattgtaatc cgcccatacc ggtatgggcg aatgaaaatt
    11701 cgcccgccgg gaccggtcac gcggcggagt tcaggatgct ttcgatgtca tcga
//
LOCUS       DAOPPA010000033        11749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6306_length_11749_cov_3.894989, whole genome shotgun sequence.
ACCESSION   DAOPPA010000033 DAOPPA010000000
VERSION     DAOPPA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6306_length_11749_cov_3.894989"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKH54_01915"
     CDS             complement(<1..222)
                     /locus_tag="PKH54_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98671.1"
                     /translation="MNTRSLHFLYVLLLGLGLPVAASAGGFEYGPQGLHAVGRGGAFT
                     VAADDPTAVFWNPSRLALMDGTHVIVNLSM"
     gene            442..1443
                     /locus_tag="PKH54_01920"
     CDS             442..1443
                     /locus_tag="PKH54_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98672.1"
                     /translation="MNKFAFIPVVALLFLVGCPGGNGNDEDVKDQDVHYDVAPNDAAH
                     EVAPLDQGGNADVAASGCLACLAPGQTFLFRTLDVTEPATPMELPNLLNTIWAPDILE
                     YRLNIMMKVEEVLPQQDGTLKMRASAGSAWHNLSFEDVRTLGNTLVPDNFHFTVPELV
                     KDIWFSIDANCDFITLNDPISGDMPGINFRPGTVEKYLLCSAGNEAIDLPPHTVPIRG
                     LYATGHFNSDCTKIEDANLSGCIAKDAACELCNMVTAPDYDLWESTSPDPSVEAVPCD
                     ASYCKHHCGKNPWTNFGGFVLGVGVPLNCDLDGDTVNEGFRLTAGFWAQGVTYAEPL"
     gene            1505..3403
                     /locus_tag="PKH54_01925"
     CDS             1505..3403
                     /locus_tag="PKH54_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC98673.1"
                     /translation="MKAAPFIIGFSMSMACSVAIAQELETLMVPVDAETGLATDVWLP
                     ALEYPEAGWPVILRRTPYGRSMARDDVSGFSSMGFVFVSQDVRGRGDSDGHFLPFLVD
                     AADGRATIEWAASQPWSNGRVVLWGGSAEGVVQLLAAGEGPVGLAGAIPVMATDDVWE
                     GMMAGGVWRKELTGAWLAGMDADDVAAEWRANVAPGEYWDRGRLTAAEMARIDVPMFI
                     IGGFFDIFAHRQPGMHRELVANVSSDAKGDQFLVYGPWTHGAFNFKDDEPVTQGQVEF
                     TRGASYDLFWFELMDFLEWTLRDGDRPSWAPVRYFLTRLSDDGVHATGTFRTLDVWPP
                     EGRPAILAICADGRLAADGCEEPAGWRGIPVDPSSPVPSVGGNNLTTAAGIFDQSLVD
                     SRPDVMFFQTAPVESDIRIQGDFFARLTVMFSGTDGDVIVRLSQVTPAGKVMLLADGA
                     VRGSFIADRLHPAAVEPGSPIDVSIPLSPVAIELPAGHSLRLSIAGTSAPRYEPNPGA
                     FGASGAAFEPAESTLSVLTVESHLALSVTSGDAWQVVEEGHDACSCGEVENGRDLIEP
                     PECPETGDVQCGCDSGEPASCPECGQTACQGCSIAGRPVAPGSVLLLMVFCVASVAAC
                     RFRGRRRF"
     gene            complement(3362..6427)
                     /locus_tag="PKH54_01930"
     CDS             complement(3362..6427)
                     /locus_tag="PKH54_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC98674.1"
                     /translation="MNIARFSLKNRVIVWMLLVTMAAWGIFTYRTISRREDPEIKISY
                     AIVFTIWPGKGAEDVERMVTTKLEDEIEKMGTLNELSSVTRENISLIIVNINFNEDAN
                     VQWQRLRNAIDEAGPNLPAGIIGPTVIDDLGDITSMIYSLRSETASPSELKEWATRLK
                     NEIKAVDAVSKVNLLGEQEEVIYIEGPMESFGLYKFSPLTAAKILDFTNVNLPAGYVR
                     YQEQDLRLDTSGAFKAVEQIENAVIDVSRESGIPLKVRDVFSVRKAYREPPVSWMTTL
                     GEKSIGLDIRMADGFNVVQMGKDVRAVVEKFRHSLPDHITLELLHDQPREVDDFIGMF
                     MDNLIEGIAIVILVMLLLMGIRSTMIVAISLPLCILSTIALMPVFNIDLEMMSIASFI
                     VAIGMLVDNSIIIIDNIYSYMERGVPADEAACQATQELQGPALTGTLATIFAFVPLLL
                     LKAEIGAYVRSLPFIITISMLFSWVIAVTIIPILARAFVRVKPISEEKRKSGRVVRMY
                     SGALRFGLKMRWAVVGITAAMLAGALLLVPVVGMSFFPEWDRDQFIIDVKLPEGAGIE
                     KTAAVVRQVEERLSTEPDIVNFASFIGEGGPRFQIAVWPEFNAPNYGRIIVNTRAKEV
                     TRPMVERLRNEFRTTIAEARVAPSNLLLAIPVEAPIAIKIEGPDIDVMRSISIEVQRI
                     LKETPGTDMIRDDLGTEIQSLKVNVDTEAAIMAGISNTEVALALLTAHEGLPVTEIRS
                     HEEKIQVALRSNEAARRENRTLDELRVTSMTTGAKVPLSAIASIEPSWDTGVIHRRDN
                     KRVVTVLAGVNGALASEVMEVAWPKIQAIELPAGYRIGSEGEEKERNAAFDQLIVIFV
                     IIIFALMFMLTIQFKSIRKALVILASVPLAIIGAMLGLLSSGYSLGFMEFLGIVSLAG
                     MVIKNAVVWVEFVDKWVSEGHRFDESIIAAGIGRFRPIVLTAGTTVGGLMPLALFGGA
                     LWEGIAWAMIGGLAVSTVLTLYVIPVIYFLAFRKMYSEAAAAPEPAGCD"
     gene            complement(6424..7467)
                     /locus_tag="PKH54_01935"
     CDS             complement(6424..7467)
                     /locus_tag="PKH54_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC98675.1"
                     /translation="MKKFEYVGFVTAAVIILAGTVHLAGCKDDAGGQVTLPPVAVRVM
                     NVVAGQQKTVKRFSGYTYPWDAAGVGFLIGGRVTSIKVKEGDHVRKGDLLATIAPEDY
                     QLVKDLTETQVEAIKPNYERVLGLVRDEAIPKAKLDEVEGMYKAAVTQQKQAERQVSY
                     TRLKAPIDGVVINRGTAVGQVIGPGMPAAIVIDISRMKVKFGVTQKELELFTVGAAVS
                     LAMDGIEGTVEGTVYHVALIPDVTTRMYEVTVAADNADERIRPGMLARVELEVRRSEG
                     IFVPLRAIKRNHEGRHVVFTVGEGDVVVERAVTTGELFGDQMKIDEGLSVGDRVILEG
                     QAFVTPGDKVKVL"
     gene            7722..9146
                     /locus_tag="PKH54_01940"
     CDS             7722..9146
                     /locus_tag="PKH54_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98676.1"
                     /translation="MNLASRSLILSAVLSLVIVTACKDSDPDTDNGFNVPDSGDWYDT
                     TGGDTSGSDTGERDGFAPDIAGQDLVGMDLVGVDLNGHDTKPIDVMSTIDTTYWDTQE
                     YGDGYAELDLAREDLMHYDLGPEDEGSRPDYGACGEPGNCNPCGYGSVTGMTCAVGTN
                     VGLPYAHVWIETTDCAGNPIKIEDYSDYQGYYVLDNVPCGTQTIHMEKGNFKHTFSRW
                     IDKCVTTDVTSKDGCFPGQPARIAVVTGKWDVIEKTLIKLRFQHKQFDGLMDGPDAAM
                     PQGGLLLTGREANTNTGVKNLTLIDDFDMVFIDCGDSPDYIMQKDGNKVMETLRAFVA
                     KGGSVYASDYASVYVTQTWPSSTSGLTFPRDFPGTAGTTPRQVTADLPDPELAAYMQK
                     PNAIVEYRLGPLTSIVGTPPAGTKVHVRGPNSSHANQMEPFMISWEPDGEAGGRVIIT
                     NFHNEEQLSTGDMAWVLQYVVFLM"
     gene            complement(9296..9371)
                     /locus_tag="PKH54_01945"
     tRNA            complement(9296..9371)
                     /locus_tag="PKH54_01945"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9336..9338),aa:Lys,seq:ctt)
     gene            complement(9560..10033)
                     /locus_tag="PKH54_01950"
     CDS             complement(9560..10033)
                     /locus_tag="PKH54_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOC98677.1"
                     /translation="MIRKLHEIVDRHFAHAVESAPDQFACRPGCGDCCLVDLSVFPVE
                     AALVRQAFAELPDATRKAAAERASLARHCCMLDPADGRCIVYHARPVICRSHGLTVLI
                     DGNLDHCPKNYGGMPATRDCILDLEKLNTALVSVNMASGGGANRVRMADIALESK"
     gene            10204..11547
                     /locus_tag="PKH54_01955"
     CDS             10204..11547
                     /locus_tag="PKH54_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOC98678.1"
                     /translation="MDPKRDLGNAPISGLLFRFAIPSIVALMVHAGYNIIDRIFIGQM
                     VGPDGLAAAAACFPAMMAQFAFTLLIAVGEAAIVSIKLGQGDTDGARKVFANAVWMSA
                     LLSILMPFAGLFLLDPILHLSGAGPATLPLARQYLSTILVGTLPLTGSFILHYHVRAA
                     GHPRAAMILIIIGSGMNAILDPIFIGPLGMGVQGAAIATVISETLSMIAGIVWLATHR
                     STPSLSRGLMGPDLKLWGSIGFLGLAPFAAQIIGSVQFSILNYQVARFGGEIAVGITG
                     VTFALSSVVSLPVFGIADGMQPIVGYNFGARKLDRVRQAVRLSLLWATVAAFIGTIVV
                     ELFPGPAVSMFSTDAEFTEMTVRAVRIVFMTAPLFGMQAIGARYFQAVGKGGLSLFLS
                     LSRQLIFFTPGLLLLPEFFGLDGVWWTAPLADTTSTAMTMGLLVWSMKKTMKEAA"
     gene            complement(11544..>11749)
                     /locus_tag="PKH54_01960"
     CDS             complement(11544..>11749)
                     /locus_tag="PKH54_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98679.1"
                     /translation="TCVQGECVKHGSTGAGGTCVTHRDCIAGTLCLDGNCLVTCSLEK
                     PCALPSACVWPCSGLDLGVCLGG"
BASE COUNT         2265 a         3521 c         3402 g         2561 t
ORIGIN      
        1 catgctcagg ttgacgatga cgtgggtgcc gtccatcagg gcaagcctgg acgggttcca
       61 gaatacggcc gtcgggtcgt cggcggccac cgtgaaggcc ccgccgcgtc cgacggcgtg
      121 aagcccctgc ggaccgtatt caaagccgcc ggcgcttgcc gcgaccggca gtccaaggcc
      181 aaggagaagc acgtaaagga agtgtaagga acgagtattc atccagtcac cattcatgtt
      241 gggagccccc cgggaccgcc tgattctggt gcataaatta tcgcgcaatt tacgattcgg
      301 caacgtaaca gtcaccgaca gttcgttcaa gacgcgttgc gtcaaattca ggcgcattgc
      361 gtcaaagtgc gcgaaaaaat aagactcctt gttactgtga agcatcgtcg tataatctgc
      421 ctgtcaatcg gaggtgtctt gatgaataag ttcgcattca taccagtggt ggccctgttg
      481 ttcctggtcg gttgtcccgg cggaaatgga aacgatgaag acgtgaagga tcaggacgtg
      541 cattatgacg tcgcgccaaa tgacgcggcg catgaggtcg cgccccttga ccagggcgga
      601 aacgccgatg tcgcggcaag cggttgcctg gcatgcctgg cccccggcca gaccttcctg
      661 ttcagaaccc ttgacgtgac cgagccggcc actccgatgg agttgccaaa cctgctcaac
      721 acgatctggg cgccggacat ccttgaatac cgcctgaaca tcatgatgaa ggtcgaggaa
      781 gtcctgccgc agcaggacgg aaccctgaag atgagggcgt cggccggttc ggcctggcac
      841 aatctgtctt tcgaggacgt caggacgctg ggcaacacgc tggttcccga caacttccac
      901 ttcacggtgc cggaactggt taaggacatc tggttcagca ttgatgcaaa ctgcgatttc
      961 atcacgttga acgatccgat aagcggcgac atgcccggaa tcaacttcag gccgggaacg
     1021 gtcgagaagt atctgctttg ttccgccggg aacgaggcga tcgaccttcc gccccacacg
     1081 gttccgatcc gcgggctgta cgcgaccggt catttcaatt ccgactgcac gaagatcgag
     1141 gatgccaacc tttccggttg catcgcgaag gacgcggctt gcgaactgtg caacatggtc
     1201 acagctccgg actatgacct gtgggaatcg acctcgcccg atccgtccgt cgaggctgtt
     1261 ccgtgcgacg cttcatactg caagcaccac tgcggcaaga atccctggac caatttcggt
     1321 ggattcgtcc ttggtgtcgg cgtccccctg aattgtgatc ttgacggcga cacggtcaac
     1381 gagggcttcc gtctgaccgc cggtttctgg gcccagggcg taacttacgc cgaaccgctg
     1441 tgatgtgctg acctcgcctg tttcccgccg tataatcctg caagatcatt ctatcggtga
     1501 tttgatgaag gctgccccgt tcattattgg attttccatg tcaatggcat gttccgttgc
     1561 catcgcgcag gaactcgaaa ctctcatggt gccggttgac gcggaaaccg ggcttgcgac
     1621 ggacgtctgg ctgccggcgc ttgaataccc ggaagctggg tggccggtaa tcctgcgccg
     1681 gacaccatac gggcgatcaa tggcccggga cgacgtgtcg ggcttttctt caatgggttt
     1741 cgtgttcgta tcccaggacg tccgcgggcg cggagacagc gatggccatt tcctgccttt
     1801 cctggtcgat gcggcggatg ggcgggccac gatcgagtgg gccgcatccc agccgtggtc
     1861 gaacggtcgc gtcgtgctgt ggggcgggtc ggccgaaggg gtcgtgcagt tgcttgccgc
     1921 tggcgaaggg ccggtcggac tggccggcgc gattcccgtg atggcgaccg acgacgtctg
     1981 ggaaggcatg atggccgggg gtgtctggcg aaaggaattg accggggcat ggcttgcggg
     2041 aatggacgcg gacgatgtcg ccgcggaatg gcgcgccaac gtcgcacctg gtgaatactg
     2101 ggatcggggc cggttgacgg ctgccgaaat ggcaaggatc gatgttccca tgttcatcat
     2161 cggcggtttc ttcgatatct tcgcgcaccg ccagcccggg atgcaccggg aacttgttgc
     2221 gaacgtatct tcagacgcga aaggcgatca gtttctggtc tatggcccgt ggacgcacgg
     2281 agccttcaat ttcaaggatg acgagccggt cacccagggc caggtcgaat tcacccgtgg
     2341 cgcctcatat gacctgttct ggttcgaact tatggatttt cttgagtgga ctcttcggga
     2401 cggggatcgt ccttcctggg cgccggtgcg ctatttcctt acaaggcttt ccgatgacgg
     2461 tgttcatgca acgggaacgt tcaggacgct ggacgtttgg ccgccggaag gtcggcctgc
     2521 catcctggcg atctgcgcag atggccgact ggccgcggac ggttgcgaag agccggcggg
     2581 ctggagaggc atcccggttg atccatcgag tccggttccg tcggtcggcg gcaacaacct
     2641 tacgaccgca gcgggaattt ttgaccagtc cctggtcgat tccaggccgg acgtcatgtt
     2701 tttccagacc gccccggttg aatccgacat caggattcag ggcgattttt tcgccaggtt
     2761 gactgtcatg ttttccggta ccgatggcga cgtcatcgtc aggctttcgc aggtgacgcc
     2821 agccggcaag gtcatgctgc ttgcagacgg ggcggtgcgc ggcagtttca ttgccgatcg
     2881 ccttcacccc gccgctgtcg agccgggaag tccaattgat gtgtccatac ccttgagccc
     2941 ggtggcgatt gaacttccgg cggggcattc gctgcgcctg tcgattgccg gcaccagtgc
     3001 gcccaggtat gaaccgaatc ccggcgcgtt cggtgcttca ggtgccgcgt ttgagcccgc
     3061 tgaaagcacc ctttcggtcc tgaccgtgga aagccacctt gcgctttccg taacttccgg
     3121 cgatgcctgg caggtggtcg aggaaggcca tgatgcatgt tcttgtggcg aggttgagaa
     3181 cgggcgggat ttgattgaac ctcccgaatg cccggaaacc ggcgatgttc aatgcgggtg
     3241 cgattcgggc gaaccggcgt catgtcccga atgcgggcaa accgcctgcc aggggtgttc
     3301 gattgccggt cggcccgttg cgccgggttc agtgcttctt cttatggtct tctgtgtcgc
     3361 gtcagtcgca gcctgccggt tcaggggccg ccgccgcttc tgaatacatc tttctgaatg
     3421 ccaggaaata gatcaccggg atgacgtaca gcgtaagaac ggttgaaacg gcaagtccgc
     3481 cgatcatggc ccaggcgatt ccttcccata gggccccacc aaacagggcg agcggcataa
     3541 gcccgccaac cgtcgttccg gcggtcagga cgattggcct gaagcgcccg attcccgccg
     3601 caatgataga ttcgtcgaac cggtggcctt cagagaccca tttatcgacg aattcgaccc
     3661 agacgacggc gttcttgatg accatgccag ccagcgagac gattccaagg aactccatga
     3721 aaccaaggct gtacccgctg gaaagaaggc caagcatggc gccgatgatt gccagcggta
     3781 cgcttgccag tatgaccagt gccttccgga tggacttgaa ctggatcgtc agcatgaaca
     3841 taagggcgaa gatgatgatc acgaagatta cgataagttg gtcgaacgcc gcgttacgct
     3901 ccttttcctc gccttcggat ccgatcctgt acccggcagg aagttcgatt gcctgaatct
     3961 tcggccaggc cacctccatc acttcggagg caagggcgcc gttcacgccg gccaggacgg
     4021 tcacgacgcg cttgttgtcg cgcctgtgaa tcaccccggt atcccatgaa ggctcgatcg
     4081 acgcgattgc cgaaagcgga accttcgcgc cggtcgtcat cgaagtgacc cgcagttcat
     4141 cgagcgtcct gttttccctg cgggctgcct cgttcgaccg aagggcaacc tggatctttt
     4201 cttcgtggct gcgaatctcg gtgactggca ggccttcgtg cgcggtcagc agcgccagcg
     4261 cgacttccgt gttggaaatg cccgccatga ttgccgcttc agtatcgacg ttgaccttaa
     4321 ggctctggat ttcagtgcca aggtcgtcgc ggatcatgtc cgttccgggc gtttccttaa
     4381 gaatgcgctg cacttcaatg cttatcgacc gcatcacatc gatgtccgga ccttcgatct
     4441 taatcgcgat cggagcttcg acgggaatgg ccaggagcag gttcgacggc gctacccgtg
     4501 cttccgcaat cgtcgtcctg aattcgttgc gaagccgttc gaccatcggc cgcgtaactt
     4561 ccttggccct ggtgttcacg atgatgcgac cgtagttcgg cgcgttgaat tccggccaga
     4621 cggcgatctg gaagcgcggg ccgccttccc cgatgaacga ggcaaagttg acgatgtccg
     4681 gctcggttga aagacgttct tcgacctgtc ggacaacagc ggccgtcttc tcgatgccgg
     4741 cgccttccgg cagctttacg tcgataatga actggtcgcg atcccattcc gggaagaacg
     4801 acatgccgac gaccggcacc agaagcaggg cccctgcaag catcgccgcg gtgatgccga
     4861 cgacagccca cctcattttc aaaccaaagc gaagcgcacc tgaatacatc cgcacgaccc
     4921 gcccggactt gcgcttttcc tcgctgatcg gtttaaccct gacgaatgcc cgggccagga
     4981 tcgggatgat cgtgaccgcg attacccacg agaacagcat ggatatcgtg atgatgaaag
     5041 gcagcgacct gacgtaggcc ccgatttcgg ccttcagcag aagcagcggc acgaaggcga
     5101 agattgttgc caacgtcccg gtcagtgccg gcccctgcag ttcctgggtg gcctggcacg
     5161 cggcttcgtc ggccggcacg ccccgttcca tgtacgaata gatgttgtcg atgatgataa
     5221 tcgaattatc gacaagcatt cctatcgcca cgatgaacga agcgatgctc atcatttcca
     5281 ggtcgatatt gaacaccggc ataagggcga tcgtcgaaag gatgcacagc ggcaggctga
     5341 tcgcgacgat catcgtcgaa cggataccca tcagaagcag catcaccagg atcactatgg
     5401 cgatgccctc gatcaggttg tccatgaaca tcccgatgaa gtcatcgact tcccttggct
     5461 ggtcgtgcag aagctcgagc gtgatgtgat ccggcaggga atggcgaaac ttctcgacca
     5521 cggccctgac gtcctttccc atctggacca cgttgaaacc atcggccatt ctgatgtcaa
     5581 ggccgatgct tttttctccc agtgtggtca tccacgaaac aggtggttcc cgatacgcct
     5641 tcctgaccga aaaaacgtcc ctgaccttca gtggaatgcc tgattcgcgg gacacgtcga
     5701 ttacggcgtt ttcaatctgc tccacggcct tgaaagcacc ggacgtgtca aggcgaaggt
     5761 cctgttcctg gtaccgcaca tagccggccg gcaggttcac attggtgaaa tcaagtattt
     5821 tcgcggcggt cagcggcgag aatttgtaaa gaccgaagct ctccatcggc ccttcgatat
     5881 agatgacctc ttcctgttct cccagaagat tcaccttgct gaccgcgtca accgccttga
     5941 tttcgttctt cagcctggtt gcccactcct tcagttccga aggtgatgcc gtttccgacc
     6001 gaagcgaata aatcatcgaa gtgatgtcgc caaggtcgtc gattacggtc ggcccgatta
     6061 tgccggccgg caggttcggc ccggcctcgt caatcgcgtt gcgcagccgt tgccattgca
     6121 cgttcgcgtc ttcgttgaag ttgatgttga cgataatcag ggaaatgttt tcacgggtta
     6181 ccgacgacag ctcgttcagg gtgcccatct tctcgatttc gtcttcaagc ttcgtcgtca
     6241 ccatgcgttc gacatcttcc gcgcccttgc ccggccagat ggtgaagacg atcgcgtacg
     6301 aaatcttgat ttccgggtct tcacgccggc tgatggttcg gtacgtgaag atgccccagg
     6361 ccgccatcgt gaccagcagc atccagacga tgaccctgtt cttcagcgag aatcgggcga
     6421 tgttcatagg accttcacct tgtccccggg cgtgacgaac gcctggcctt ccaggatcac
     6481 cctgtcaccg accgaaagtc cttcatcgat cttcatctga tcgccgaaca attcgcctgt
     6541 cgtgaccgcg cgttcgacga cgacgtcacc ttcaccgacg gtgaagacga cgtgccgtcc
     6601 ttcatgattt ctttttatcg cgcgcagcgg cacgaagata ccctctgagc ggcgcacttc
     6661 caattcgacc cttgccagca tgccggggcg gatcctctcg tcggcgttgt cggccgcgac
     6721 ggtgacctcg tacatccttg tggtgacgtc cgggataagc gccacgtgat agacggtacc
     6781 ttcaacggtt ccttcgattc catccatggc cagtgacacc gccgcaccga ctgtgaaaag
     6841 ttcgagttcc ttctgggtga ccccgaattt tactttcatc ctggaaatgt cgatgactat
     6901 cgctgccggc attcccgggc cgatgacctg gccgaccgcc gttccccggt tgatcacgac
     6961 gccgtcgata ggggccttca gcctggtata tgaaacctgg cgttcggcct gcttctgctg
     7021 cgtgacagcc gccttgtaca tgccctcgac ttcgtcgagc ttcgccttcg ggattgcctc
     7081 atcccgcaca agtccaagca cgcgttcgta attcggcttg attgcctcga cctgggtttc
     7141 cgtcagatcc ttgacaagct ggtaatcctc gggggcgata gtggccagaa ggtcgccctt
     7201 cctgacgtga tcgccttcct tcaccttgat tgacgtcacg cgaccgccga tcaggaatcc
     7261 gaccccggcg gcatcccagg gatacgtgta tcccgaaaaa cgcttgacgg tcttttgctg
     7321 gcccgccacc acgttcatga cgcgaaccgc caccggtggc aaggtcacct ggcccccggc
     7381 gtcatccttg cagccggcaa ggtgcacagt gcccgcaaga attatgacag cagcagtcac
     7441 gaaaccgaca tattcaaact tcttcatagg ttgacctgca aaggattggc accggtctgc
     7501 cgcctgaaag cccgatcggg cccgtcacac gcggccgcga atcgattata aaccgtcatg
     7561 gaacgtttcg aaaccccggg gaatggacat cggaatggcg cgcagtgcgc cgtcctttgt
     7621 ggcgcgtcgc gtcaaaagtt ttgaagaaac aagacatttc cgcgtattga ctaaaaaaat
     7681 ccaggttcgt atcctactcc gtaacccttg gggatttaac catgaacctt gcttcgcgtt
     7741 cattgattct gtcggctgtc ctgtcgctcg tgatcgtcac cgcgtgcaag gactcggatc
     7801 cggatacgga caacggtttt aacgtgcccg actcgggcga ctggtacgac accacaggcg
     7861 gcgatacaag cggctcggat accggcgagc gggacggatt cgcacctgac attgccggcc
     7921 aggatcttgt tgggatggat cttgtcgggg ttgacctgaa cggccatgac accaagccga
     7981 tcgatgtaat gtcaacgatc gacacgacgt actgggacac ccaggaatat ggcgacggtt
     8041 acgccgaact ggatcttgcg cgcgaggatc tgatgcatta cgacctgggg ccggaggacg
     8101 agggatcccg tccagactat ggcgcctgtg gcgagccggg caactgcaat ccgtgcggat
     8161 acgggtcggt gaccggaatg acctgcgccg tcggaaccaa cgtcggcctt ccgtacgccc
     8221 acgtctggat tgagacgacc gactgcgccg gcaacccgat aaagatcgag gattattccg
     8281 actaccaggg ttactacgtg ctggacaacg tcccgtgtgg aacccagacc attcacatgg
     8341 aaaagggtaa tttcaagcac acgttctcgc gctggatcga caagtgcgtg acgaccgacg
     8401 tcacttcgaa ggacggttgc ttcccgggcc agcccgcccg aatcgccgtt gttaccggca
     8461 agtgggacgt catcgaaaag accttgatca agctgcgttt ccagcacaag cagttcgacg
     8521 gcctgatgga tggtccggac gcggccatgc cccagggcgg cctgctcctt accggccgcg
     8581 aggcgaacac gaataccggc gtgaagaacc ttaccctgat cgacgatttc gacatggtgt
     8641 tcatcgattg cggcgattcg ccggactaca tcatgcagaa ggacggcaac aaggtcatgg
     8701 aaaccttgag ggcgttcgtc gccaagggcg gctcggttta tgcgtccgac tacgcctcgg
     8761 tttatgtcac ccagacctgg ccttcctcaa cctccggact gaccttcccc cgcgattttc
     8821 ctgggacggc tggtacgacc ccgcgtcagg tcacggcgga tctcccggat cccgaacttg
     8881 ccgcatacat gcagaagccc aacgctatcg ttgagtaccg cctgggacca ctcacgtcga
     8941 tagttggaac gccgcctgcc ggcaccaagg ttcacgttcg cgggccgaat tcctcgcacg
     9001 ccaaccagat ggaacctttc atgatttcct gggagccgga cggcgaggcc ggcggccgcg
     9061 tcatcattac caatttccac aatgaagagc agctttcgac gggcgacatg gcctgggttc
     9121 ttcagtacgt cgtcttcctg atgtagattc cgaagcaagt tcccgaatca attgcagtag
     9181 caggaagaaa tcaggaaaaa cgtcatcttg ctgtaatcgg gagaccggtt tgcgcgggca
     9241 aaaaaaaggc acccccgttc ggagatgcct ggaagcgtca tttgttttga cgcactggtg
     9301 agcccagaag gaatcgaacc ttcaacctcc ggattaagag tccgctgctc taccgattga
     9361 gctatgggcc cagcgcaaaa gcggaactat tgtcgttgca taaggttcat ttgtcaagcg
     9421 atttgttgcc ataacgttcc caccgttcgc acgggcgctg ttcggatctg tccttgtacg
     9481 ggcggattac atccgcccgc tgcggttcag tacggtgcct ttaatcgcgc cgctggttcc
     9541 ggcctgtccc ttctcgtgtt tatttgcttt ccagggcgat gtcggccatg cgcacgcggt
     9601 tcgcgccgcc gccggaggcc atgttcaccg acaccagggc cgtgttcagc ttttccagat
     9661 ccagaatgca gtcacgcgtg gccggcatgc cgccgtaatt cttcgggcag tggtcaaggt
     9721 ttccgtcaat taagaccgtc agcccgtggc ttcggcatat gaccgggcgg gcgtgataca
     9781 cgatgcaacg tccgtcggcc ggatccagca tgcagcagtg ccgggcaagg gaagcccgtt
     9841 cggctgcggc ctttctggtc gcgtcgggca gttcggcaaa cgcctgtcgg acaagggccg
     9901 cctcgaccgg aaagaccgaa agatcgacca ggcagcagtc gccgcacccc gggcggcacg
     9961 caaactgatc cggtgccgat tcgaccgcat gcgcgaagtg ccgatcgacg atttcgtgaa
    10021 gctttcggat catggttctt cccgctgcat tcccgctgcc acagtcagca cggatcatgc
    10081 cagacgtcgt gattcatgtc atgccgcctt tgattattga attcatgttg gctacacttc
    10141 cgcccgtcca gaccggggcc gtaccccgat ccgttccctg gcgaatatca aggcggcatt
    10201 cctttggatc cgaagcgcga tcttggcaac gcaccgatat cgggactcct gttccgattc
    10261 gcaatcccgt ccatcgtggc cctgatggtt cacgccggtt acaacatcat cgaccggatc
    10321 ttcatcggcc agatggtcgg ccctgacggg cttgccgccg ccgccgcatg cttcccggcc
    10381 atgatggccc agttcgcctt taccctgttg attgccgtcg gcgaggcagc catcgtttcg
    10441 atcaagctgg gccagggcga caccgacggt gccaggaagg tgtttgcaaa cgccgtctgg
    10501 atgtcggcgt tgctttcgat tctgatgccg ttcgccggcc tgttccttct tgatccgatt
    10561 cttcaccttt ctggagccgg tccggcgacc ctgccactgg cccggcagta cctttcaacc
    10621 atccttgtcg gcacgctgcc gctgaccggc agcttcatcc ttcattatca cgtgcgggcg
    10681 gccggtcatc cgcgggccgc catgatcctg atcattatcg gatccggcat gaacgccatc
    10741 ctggatccca tcttcatcgg acctcttggc atgggtgtcc agggtgcggc catcgcgacc
    10801 gtgatttccg aaacactgtc gatgattgcc ggcatcgtct ggcttgccac gcaccgttcc
    10861 acaccttcac tttcccgcgg acttatgggg ccggacctga agctgtgggg aagcatcgga
    10921 ttcctggggc ttgcaccttt cgcggcccag ataatcggca gcgtccagtt ttccatcctg
    10981 aactaccagg tggcgcgctt tggcggcgaa attgccgttg gaataaccgg cgtcacgttc
    11041 gcgctttcgt cggtcgtttc cctgccggta ttcggcattg cggacgggat gcagccgatt
    11101 gtcgggtata acttcggtgc gcggaaactt gatcgcgtgc gacaggccgt gcgcctttcc
    11161 cttctgtggg caaccgttgc cgcgttcatc gggacgatag tcgtcgagct ctttcccggc
    11221 ccggcggttt cgatgttctc gaccgatgcc gaattcaccg aaatgaccgt cagggcggtc
    11281 aggattgttt tcatgacggc gccccttttc ggcatgcagg cgattggcgc aaggtacttc
    11341 caggcggtcg gcaagggcgg gctttcgctt ttcttaagcc tttcccgcca gttgattttc
    11401 tttacccccg ggctgcttct gcttcccgag ttcttcggtc ttgacggtgt ctggtggacg
    11461 gcacccctgg cagacaccac atccacagcc atgacgatgg gtcttcttgt ctggtcaatg
    11521 aagaagacga tgaaggaagc cgcctagccc cccaggcaga cgccaagatc cagtccggaa
    11581 catggccaca cgcaggctga aggcagtgca cacggctttt ccaggctgca tgtaaccagg
    11641 cagttcccgt caagacacaa agtccccgca atgcagtcgc gatgtgtcac gcatgttccg
    11701 cccgccccgg tgcttccatg cttgacgcat tcaccctgga cacaggtgg
//
LOCUS       DAOPPA010000034        11715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6329_length_11715_cov_4.087736, whole genome shotgun sequence.
ACCESSION   DAOPPA010000034 DAOPPA010000000
VERSION     DAOPPA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6329_length_11715_cov_4.087736"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="murG"
                     /locus_tag="PKH54_01965"
     CDS             <1..1029
                     /gene="murG"
                     /locus_tag="PKH54_01965"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOC98680.1"
                     /translation="LPAVETARVIREMGFATVMVTSGQAIEKEILKNSGMEVRTLHVQ
                     KINRMGVSGLVKGALSIPGALLASATMLREIRPAAVAGFGGFTSGPLVLAAQMMLIPT
                     AVFEANSVPGITNRILARSARRVFVTFPSAGSRMGRKDFEVTGNPVRREILVHQRPEP
                     AGPASRILVVGGSQGSRFLNQNVPKVAALLKDRIDGLVLRHQCGIGNSNAVAAEYRAA
                     GVEAQATDYIHDMAEAMSWADFIICRSGAGTVGEVTAVGIPALFVPFALASDDHQAHN
                     AMDLVRAGASLMKRESEFDPASVAAELAVIMRDPERLRAMSSAALAAGRRDAAQRIAR
                     GIVSMVKA"
     gene            1026..1979
                     /gene="murB"
                     /locus_tag="PKH54_01970"
     CDS             1026..1979
                     /gene="murB"
                     /locus_tag="PKH54_01970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOC98681.1"
                     /translation="MTRLDAIYEMLCAISGIAARRDEPMSRHTTLQLGGPVDLFVQYD
                     SAPALVEAASALERESCPWFPVGFGSNLLVMDQGIEGAILVPGSPMTGIEFSHADEPT
                     VTAGCGTGIGPLLQAIGKSGYSGLEFLTGIPGTVGGAVAMNAGTRYGWIQDALISVTV
                     ASAGGMRDIATAALGLGYRRSAVPTGSVITGATFRLSRGPSEEGALVARTLSELRRNN
                     HPPASGTAGSFFRNPDPAANLFAGQLIEECGLKGFSKGGAVVSPKHANFLMNGGGATA
                     ADLLGLALLVRNRVLEEKGVALTREVRVVGRGSREWQDLLP"
     gene            1992..2924
                     /locus_tag="PKH54_01975"
     CDS             1992..2924
                     /locus_tag="PKH54_01975"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOC98682.1"
                     /translation="MAGNRKVLKPHQRIAAVLSSVGRKAWARRAWIAGSVAFMLSSAG
                     IHQAAGHLSRTGRFNLKNVEVLGAFRFDPAMVEEICGLETGVTNVFTTDADEVMDRCM
                     EDDRIEWAEVTRLLPDRIRIRIREASPVIFAAAPEGVRAFDSMGRRYFAADPDWAPGL
                     PLLTGLQQMMEPVVLAAQDDETPRQRRSRLARQAQADREHADRVGQVVAEAARLVACM
                     DTRHPDWLQGGVVVDWDEALGFELRMPDRPVISFGFEGFDEKVARIDSVLAVAMARDL
                     DVEQIYLDDDESSSSGSLRWREKGTALTSVVTFE"
     gene            2940..4187
                     /gene="ftsA"
                     /locus_tag="PKH54_01980"
     CDS             2940..4187
                     /gene="ftsA"
                     /locus_tag="PKH54_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOC98683.1"
                     /translation="MRAKREIVGALDLGSSSVALVIAEVSREDVTVLGTGIAPCEGLS
                     NGRVISINDTAAAITAARVEAEHMSGCELSEVYVSFSGRDITGMNNRGLIYHRKCAQV
                     SRGDLESAIENAIHVRLDEGVVLNVVPRQYILDRHEGIKDPVGMSGVQLEAEVHMTIA
                     DRAALDTIERCVLKASLKVRDFVVAPLASSLAVLREDERKMGVIMLDLGTGTSNIQIW
                     QHGRLEWTGVIDMGTDLITTAISRSLRTPVSCAEDIRLRFGAACLADDDVNQPIPVAC
                     VGGRGPAQISRRMLVENIIQPTVVDWLTTIASRLQTVIDFNDMTAGIVLTGAAALLPG
                     IELVSNGVFNLNTRLGAPESVGGINSRVEQNPMMSAAWGTVIYRSMLPSGDAVQPMTP
                     RSAREMPGGGFGGLISRAINFLF"
     gene            4258..5634
                     /gene="ftsZ"
                     /locus_tag="PKH54_01985"
     CDS             4258..5634
                     /gene="ftsZ"
                     /locus_tag="PKH54_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOC98684.1"
                     /translation="MAFEIVQESGKAEEAGSVKLTGRAEVVVGQDLFNGERPQALPKP
                     MPLPGAESIIKVVGIGSGGVNAVNTMIGNGIAGIDYIAVNTDMQDLQKSLAPVRIVLD
                     SSTGGRGAGGDPEKAAAAAMEKRKEIESALRDAQMVVLATGLGGGTGTGATPVIGDIA
                     RQMGILTVAVATMPFKWDGRIKALRAATGLDQLRDRVDAYFTISNDKMLKAFSGVPTG
                     DAFRSFDGVLLCEAVEGVVDLVTRPGIINLDIEDVRTALENRGACVICSGMGEGAGRA
                     EVAAEAALHNPLLDDSPVNGARNILVNIVQGPTGTIDDIGVIMNHVLEMMDPDCFVKT
                     GFATDPSLGEKVKVTIVASGIDVTPVVPAELSVIDGELDFQSGARPIPFAEALPAQQG
                     SGIARKLSSSMERLGFSVKHNQNEVNVDAGMLFSNVGGSEFDAPAYTRQTGEMRAFPS
                     GSGKIDVR"
     gene            complement(5707..6948)
                     /locus_tag="PKH54_01990"
     CDS             complement(5707..6948)
                     /locus_tag="PKH54_01990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOC98685.1"
                     /translation="MPKNRIFDLHTHPSLRLYYMPWISKTFHTTPYYSGPHFNPFEFR
                     TRYANLDRSPVKVLMNAHYVIEYNFLRHGFTRAARGAFWAFAAGYYAKLLIADPWKTL
                     VKMIRVLNESVDTTNRLLVPGQSHRLAIVDSMDAVDRLDGDQIGIIHAVEGAHSIGAG
                     LEKLPTETRNAVTRERLRLLKSLGVACVCPSHFYDNPYSPQVESTEIIPRKIHGRIIA
                     TRDPAITMRRAEWHWDDPDHSAEWFFNELFRMGMAVDLSHTREDARWKVYDIAEAWKR
                     PVMISHTGLRHFFNHEYNASDEEVRRVRELNGVVGLILSRRWLVDPETRFYSGNDGIQ
                     DLIQNMLHIRNICGDVEAIGLGTDFDGMTHPFSDCFKPNQLDRVIHAMKPHFTEQETD
                     KILYGNAVRVMKDSWVPESKS"
     gene            complement(7032..7814)
                     /locus_tag="PKH54_01995"
     CDS             complement(7032..7814)
                     /locus_tag="PKH54_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98686.1"
                     /translation="MKTPVILRLASLIAIVTASAAIGCGDSVANRGNLGNLVYTLFTQ
                     YEIEETELSAVSLITGVQHRIVVTGAPGADIKQRSTISHILSPANDNEIVTDTPTDDA
                     SIGDALVTVNLPGTYTLQSWKETELVDYIVLKFVEASDLRVVIFLKLPESQEFAHAAG
                     DFLTVPLWTQATFLAAPVDADGNDLVGDIGLEITTSPQDSLIRLSNLISVTEMDVWES
                     AYPINMLFVETGTIQVTFTEPLTGLSKVLTFNVTTDYVPPDE"
     gene            complement(7891..9111)
                     /locus_tag="PKH54_02000"
     CDS             complement(7891..9111)
                     /locus_tag="PKH54_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC98687.1"
                     /translation="MNDRIIYLDNNATTPVADEVLQAMIPFLKEQFFNPSSTYPQARK
                     AAEAVEAARESVARLLGAGSKSEIIFNGGATEGNNSAIWGAARANPARRHVITTAVEH
                     PAVLEVVREMEREGFRATYLPVNRHGMLDAADLIRAIQDDTAVVSIMHANNETGVIFP
                     IGELAALVKKVDPDVVFHTDATQTAGKMRLDLQGELKNVDLLTISGHKLHAPKGVGAM
                     FVRRGSRWRPWQLGGHQERGRRAGTENVASIVGLGAAARLVMTHPNHSTAMRDRLEKA
                     IKDQIPNLFINGEGAPRLANTTNVAFEFVEGESILLALNDHGICVSSGSACTSGSLDP
                     SHVLKAMAVPFTAAHGSLRISTSMFSTDDEIDAVIDVLPTVIARLRAISPYWDVRTNS
                     PRTDVDIFIQGKYR"
     gene            complement(9130..10092)
                     /locus_tag="PKH54_02005"
     CDS             complement(9130..10092)
                     /locus_tag="PKH54_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOC98688.1"
                     /translation="MPWDYSEKVLQLFRDAMAAKAGTHMGSIDDPDGIGSHGSLSCGD
                     ALEFAFKVDATEPDPRRQKIVQARYRTFGCTSAIASSEALCALIEEKGLTPIQALKIT
                     NADIVDYLDGLPAQKLHCSVMGAEALQAAVADWAHKRNIPLSELGLDAIPTGEDEGRI
                     VCRCFNLTEPFIRRKVKELNLKTIADITNAIKAGGACTACHHEPGGLQDILDDTWGRS
                     DSSQAATPAITDPQPMPQTSSSMTRTPFQLAREVDRVVESDIRPRLQHDGGDLEIVEI
                     RGKKVFCRLTGGCQGCKGAPQTMRLVVEDALRAKVDAELEIVAI"
     gene            10247..11215
                     /locus_tag="PKH54_02010"
     CDS             10247..11215
                     /locus_tag="PKH54_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOC98689.1"
                     /translation="MESIGTPLLWVGFVVFVLIMLAVDLGVFQRKAHSVSMREAGLWT
                     VVWIALALIFNVGVYHWFGYAHAMEFLTGYLMEKALSMDNLFVFLVLFSYFGVPGHLQ
                     HRVLFFGILGALIMRALFIVAGVALISKFHWVMYIFGAFLVFTGIKLVMNKGGEVHPE
                     RNFMLRIFKKFVPTVPEYRGTHFVVREGGRLMATPLLAVLVVIEGTDVLFAVDSIPAV
                     FGITRDPFIVFTSNIFAILGLRALYFLLAGIVEKFRFLKYGLGLVLAFVGFKMLVEDL
                     LHHMHREIPIAMSLAVIAGTVGLSILISLIIPPKADSRPGDQSAGQ"
BASE COUNT         2181 a         3376 c         3699 g         2459 t
ORIGIN      
        1 ctgccagccg tggaaacggc ccgcgtcatc cgtgaaatgg gatttgccac ggtcatggtg
       61 accagcggcc aggcaatcga gaaggaaatc ctgaagaact ccggaatgga agtccgcacc
      121 cttcatgtcc agaagattaa ccgcatgggc gtttcaggcc tggttaaggg cgcgctgtcg
      181 attccgggcg cgcttctggc ctcggcgacg atgcttcgcg aaatccgccc cgcggccgtc
      241 gccggcttcg ggggtttcac atcgggcccg ctggttcttg ccgcgcagat gatgctgatt
      301 ccgacagcgg ttttcgaggc caacagcgtg cccggcatca caaacaggat tcttgcaagg
      361 tccgcccgaa gggtcttcgt gacgtttccg tccgccggtt cgaggatggg aaggaaggac
      421 ttcgaggtca ctggcaaccc ggtgcggcgc gagatcctgg ttcaccagcg gcccgagccg
      481 gccggcccgg ccagccggat acttgttgtc ggagggtccc aggggtcgcg cttcctgaat
      541 cagaacgttc cgaaggttgc cgcactcctg aaagaccgca tcgacgggct tgtcctgagg
      601 caccagtgcg gaatcggcaa ttccaacgcc gtcgcggccg aataccgggc cgcaggggtc
      661 gaggcccagg caaccgacta cattcatgac atggccgagg cgatgtcatg ggccgatttc
      721 atcatctgcc gatccggcgc gggcactgtc ggtgaagtga cggcagtcgg gattccggcc
      781 cttttcgtgc cgttcgccct ggcgtcggac gaccatcagg cccataacgc catggatctt
      841 gtcagggccg gcgcatccct tatgaagagg gagtctgagt tcgacccggc ttcggtcgcc
      901 gcggaacttg ccgtgatcat gagggatccg gaacgcctga gagcgatgtc ttccgcggcc
      961 ctggccgccg gcaggcgtga tgcggcacag cggatcgcgc gtggaatcgt gtccatggtg
     1021 aaggcatgac gagactggac gccatttatg aaatgctttg cgccatcagt ggcatcgcgg
     1081 ctcgccgcga cgagccgatg agcaggcaca cgacccttca gcttggcggt cccgtcgatc
     1141 tctttgtcca gtacgactcg gcaccggcac tggtcgaggc agcgtcggct ctggagcgtg
     1201 agtcctgccc ctggttcccg gtcggcttcg ggtcgaacct gctggtaatg gatcagggaa
     1261 tcgagggcgc gatcctggtg ccgggatcgc cgatgaccgg aatcgagttc agccacgccg
     1321 atgagccgac cgtcaccgca ggatgcggaa ccggaatcgg gccccttctc caggcgattg
     1381 gcaagtccgg atactcgggg ctggaattcc tgaccggaat ccccggaacc gtcggtggtg
     1441 ccgtggccat gaacgccgga acccggtacg gatggatcca ggacgccctg atctccgtta
     1501 cggtcgcctc ggccggcggc atgcgggata tcgcgacggc cgcccttggg ctgggttatc
     1561 gcagatcggc ggtgccgacg ggatcggtca tcacgggcgc gacgttccgc ctgtccaggg
     1621 ggccttcgga ggagggagcc ctggtggcaa ggacgctgtc cgagcttcgt cggaacaacc
     1681 atccgcctgc cagcggaacc gcgggatcct ttttcaggaa tccggatccg gccgcgaatc
     1741 ttttcgccgg ccagcttatc gaggaatgcg gattgaaggg attttcaaag ggtggcgcgg
     1801 ttgtttcgcc aaaacacgcg aacttcctga tgaacggcgg tggtgccacg gcggcggatc
     1861 tgcttggcct ggcacttctt gtaaggaaca gggtgcttga agagaagggt gtcgccctga
     1921 caagggaagt gagggtggtc gggcgcgggt cacgggagtg gcaggatctg ctgccgtaac
     1981 ggaggttcag gatggctggc aatcgcaagg tactgaagcc gcatcaaagg attgccgcgg
     2041 tgctttcgtc cgttgggcgc aaggcctggg cgcgcagggc gtggattgcg ggctccgtcg
     2101 cgttcatgct gtcctccgcg ggaatccacc aggcggccgg gcatctgtcc aggaccgggc
     2161 ggttcaacct gaagaacgtg gaagtgctgg gagccttccg tttcgacccg gcgatggtcg
     2221 aggagatctg cggacttgag accggggtca cgaatgtatt cacgaccgac gccgacgagg
     2281 tcatggatcg ctgcatggaa gatgatcgca tcgagtgggc tgaagtgacg cgccttcttc
     2341 cagacaggat ccggattcgg attcgcgagg caagtccggt gattttcgcc gcggccccgg
     2401 aaggcgtcag ggcctttgat tcgatgggcc ggcggtactt cgcggcggat ccggactggg
     2461 ccccgggcct tccgctgctg accggtcttc agcagatgat ggagccggtt gtgctggccg
     2521 cgcaggacga tgagacgcca aggcagagaa ggtcccgcct ggcgcgccag gcacaggccg
     2581 accgcgagca cgccgaccgt gtcggccagg tcgttgcgga ggcggcgcgc ctggtggcct
     2641 gcatggacac caggcatccc gactggcttc agggcggcgt cgtcgtggac tgggacgagg
     2701 cactcggctt cgaactaagg atgccggacc ggccggtcat ttcgtttgga ttcgagggat
     2761 tcgatgagaa ggtcgcgcga atcgactcgg tgctggccgt ggcgatggca agggatcttg
     2821 atgtggaaca gatatacctt gatgacgacg aatcgtcttc atcgggttct ctcaggtggc
     2881 gtgaaaaggg tacggcgctg acgagcgtcg tcaccttcga gtgattcgag gagggcagga
     2941 tgagggcaaa acgggaaata gtcggcgcgc ttgaccttgg aagttcttcg gtcgccctgg
     3001 tgattgccga ggtgtccagg gaggatgtca cggtccttgg aaccggcatc gcgccatgcg
     3061 agggcctgag caacggacgc gtcatctcga tcaacgacac ggcggccgcg ataaccgcgg
     3121 cgcgggtcga ggcagagcac atgtccggct gcgaactgtc cgaagtctat gtcagtttca
     3181 gcggccggga catcaccgga atgaacaacc gggggctgat ctaccatcgc aagtgtgccc
     3241 aggtatcccg cggggatctt gaaagcgcaa tcgaaaatgc catccacgtg cgccttgacg
     3301 agggcgtcgt cctgaacgtt gttccgcgcc agtacatcct tgacaggcat gaaggaatca
     3361 aggatccggt cggcatgtcg ggtgtccaac tcgaggccga ggtgcacatg acgatcgcgg
     3421 atcgcgcggc cctcgacacg attgagcgtt gcgtcctgaa ggcatcgctg aaggttcggg
     3481 acttcgttgt ggcacccctg gcgtcttcgc ttgccgtcct gcgcgaggac gaacgcaaga
     3541 tgggcgtcat catgcttgac ctcggaaccg gcacttcgaa catccagatc tggcagcacg
     3601 ggcggctgga atggaccggc gtcatagaca tgggcaccga cctgatcacg accgcaatca
     3661 gccgatcgct gcgaaccccg gtttcgtgcg ccgaggatat caggctgcgc ttcggcgcgg
     3721 cctgccttgc cgacgacgac gtcaatcagc ccatcccggt cgcgtgcgtc ggtggtcggg
     3781 ggcctgccca gataagccgg cgaatgcttg ttgaaaacat catccagccg acggttgttg
     3841 actggctgac cacaatcgcc agccgactcc agactgtcat cgacttcaac gatatgactg
     3901 ccggaatcgt tttgaccggg gctgcggcgc tgctgccggg aattgagctt gtctccaatg
     3961 gagttttcaa ccttaacacc aggctcggcg cgcccgagtc ggttggcggc ataaattcca
     4021 gggtcgaaca gaacccgatg atgtccgccg cgtggggaac ggtgatctac aggtcgatgc
     4081 ttccctccgg cgacgcggta cagccgatga ctcccagatc cgcaagggag atgccgggtg
     4141 gcggattcgg cgggctgata agcagggcga taaatttcct gttctgaacc ggtttatggg
     4201 cggccccgcc cgcaaggcgg ggtcttttac acttgatact ttgacggggt gataatcatg
     4261 gcttttgaaa tcgtgcagga gagcggcaag gccgaagagg ccggaagcgt caagctgacc
     4321 ggtcgcgcgg aggtcgtcgt cggccaggac ctgttcaacg gggagcgccc tcaggctttg
     4381 ccgaagccca tgccgctgcc tggcgccgaa tcgatcatca aggtggtcgg tatcggatcc
     4441 ggtggcgtca atgcggtcaa tacgatgatc ggaaacggga tcgccggaat cgactacatc
     4501 gcggtcaaca cggacatgca ggatctgcag aagtcgctgg ccccggtcag aatcgtgctc
     4561 gacagcagca ccggcggtcg tggcgctggc ggggatccgg agaaggcggc ggccgcggcc
     4621 atggaaaagc gcaaggaaat cgagtcggcc ctgcgcgacg cgcagatggt cgtgctggca
     4681 accggccttg gcggtggcac gggaacgggc gcgacgccgg tcatcggcga tatcgccagg
     4741 cagatgggaa tccttaccgt tgccgtcgca accatgccat tcaagtggga tggccggatc
     4801 aaggcgcttc gcgcggcaac cggtcttgat caactccgcg acagggtcga tgcgtacttc
     4861 acaatctcca acgacaagat gctgaaggcc ttttccggcg tgccgaccgg cgacgcgttc
     4921 aggagcttcg acggcgtcct gctctgcgag gccgtcgaag gcgtcgttga cctggtcacc
     4981 aggcccggaa tcatcaacct tgacatcgag gacgtcagaa ccgcactgga aaatcgcggc
     5041 gcgtgcgtca tctgttccgg aatgggcgaa ggtgccgggc gcgcggaagt cgcggccgag
     5101 gcagcccttc acaaccccct gctggacgac agccccgtga atggagcccg gaacatcctt
     5161 gtgaacatcg ttcaggggcc gacgggcacg atcgatgata tcggcgtcat catgaatcac
     5221 gttctggaga tgatggatcc cgactgcttc gtgaagacag gcttcgcgac cgatccctcg
     5281 cttggcgaaa aggttaaggt gaccatcgtc gcctctggga tagatgtgac cccggtcgtg
     5341 ccggcggaac tgtcggtgat cgacggtgaa ctggacttcc agtccggggc gcgtccgatt
     5401 ccgtttgccg aggcccttcc ggcgcagcag ggatccggca tcgcccggaa gctctcgtcg
     5461 agcatggaaa ggctcggctt ttcagtcaag cacaaccaga acgaagtgaa cgtcgatgct
     5521 ggaatgctgt tttcgaatgt cggcggaagc gaattcgacg cgccggcata cactcgccag
     5581 accggcgaga tgcgcgcctt tccgtccggt tccggaaaga ttgacgtaag gtgatacccg
     5641 atccggacac cccgagatcc ggacaccccg tggctccctg gcgaccgccc atgtcgcccg
     5701 ggggccctat gatttcgatt cgggaaccca ggaatccttc atcacgcgaa ccgcattacc
     5761 gtaaagaatc ttgtcggttt cctgctcggt gaagtggggc ttcatcgcgt ggatcacgcg
     5821 gtcgagctgg ttcggcttga agcagtcgga aaatgggtgt gtcatgccgt cgaagtccgt
     5881 tcccagtccg atggcctcga catccccgca gatgttcctg atgtgcagca tgttctggat
     5941 cagatcctgg attccgtcgt ttcccgagta gaaccttgtt tcgggatcga caagccagcg
     6001 cctggaaagg atcagcccga cgactccgtt gagttccctg acgcgtcgga cttcctcgtc
     6061 ggaagcgttg tattcgtggt tgaagaagtg ccttaacccg gtgtgggaaa tcatgaccgg
     6121 ccgcttccat gcttcggcta tgtcatagac cttccacctg gcgtcttcgc gggtgtgtga
     6181 aagatcgacc gccatgccca tcctgaagag ttcgttgaag aaccattcgg cggagtggtc
     6241 ggggtcgtcc cagtgccatt cggccctgcg catggtaatc gcgggatccc ttgtcgcgat
     6301 gatgcgcccg tgaatcttgc gcgggatgat ttcagttgac tccacctggg gcgaatacgg
     6361 gttgtcataa aaatgcgacg ggcagacgca ggccacgccg agggacttga gcaggcgaag
     6421 tcgctccctg gtcacggcgt tgcgcgtttc cgtcggcagc ttttcaaggc cggcaccgat
     6481 tgagtgggca ccctcgacgg cgtggataat cccgatctga tccccgtcaa ggcgatcgac
     6541 cgcatccatc gagtccacga tcgccagcct gtgactctgg ccgggaacca gaagtctgtt
     6601 ggtcgtatcg acggactcgt tcagaacgcg gatcatcttg accagagtct tccagggatc
     6661 cgcgatcagc agtttggcat agtagccggc ggcgaaggcc cagaaggcgc cgcgggcggc
     6721 cctggtgaat ccgtggcgca ggaaattgta ctcaatcaca tagtgggcat tcatcagcac
     6781 cttgaccggg cttctgtcca ggttggcata ccttgtcctg aactcgaagg ggttgaagtg
     6841 cggccccgaa tagtaaggtg tcgtgtgaaa tgtcttcgag atccacggca tgtagtaaag
     6901 cctcagactc ggatgcgtgt gtagatcgaa aatgcggttt tttggcatcg gtattccttc
     6961 tgttgcgacc aatgacgcaa tgcgtgatgt tactcgatct gcgtcatgaa gttgtcatga
     7021 aaatgcggga atcactcgtc cggaggcacg tagtcggttg tcacgttgaa agtcagcacc
     7081 ttggaaagac ctgtcagcgg ctcggtgaaa gtgacctgaa tcgttccagt ttccacgaag
     7141 agcatgttaa tcggatacgc gctctcccag acgtccattt cggtgaccga gatcaggttc
     7201 gacaaccgga tgaggctgtc ctgaggactg gtcgtgattt cgagtccgat gtcgccgaca
     7261 aggtcattgc cgtccgcatc gacgggcgcg gccaggaagg ttgcctgggt ccaaagcggg
     7321 acggtcagga agtcaccggc agcgtgcgcg aattcctggc tttcaggcag cttcaggaag
     7381 atcaccacgc gaagatcgga tgcctcgaca aatttcagga cgatgtaatc caccagttcg
     7441 gtttccttcc atgactgcag cgtgtacgtg ccgggaaggt tgaccgtgac cagcgcatcc
     7501 ccgatcgatg cgtcgtcggt cggcgtatcg gtgacgattt cgttgtcgtt cgccggggac
     7561 agtatgtgcg aaatggtcga acgctgcttg atgtcggcgc ctggggcccc ggtcacgact
     7621 atccggtgct gcaccccggt tatcagtgat acggcggaca gttcggtctc ctcgatttcg
     7681 tactgcgtga acagcgtgta gaccagattg cccaggtttc cgcggttggc aaccgagtcg
     7741 ccgcatccga tcgcggcaga ggcggtcacg attgcaatca ggctggccag tcgaagaata
     7801 accggagtct tcattgtcaa cctccagaat caacactcgg gacattatga cgaacaaagg
     7861 caacaaagtt caagaaaaca ggcgtgaaga tcagcggtat tttccctgga tgaagatatc
     7921 gacatcggtt ctgggggagt tggttcgcac atcccagtac ggggaaatcg cacgcagtct
     7981 ggcaatcacg gtcgggagca catcgatgac ggcgtcgatc tcgtcgtcgg tggaaaacat
     8041 tgaagtgctt atgcgcaggc ttccgtgggc cgccgtgaag ggtaccgcca tggccttcag
     8101 gacgtgcgaa ggatcaagcg agccgctggt gcaggcggaa cccgatgaaa cgcagattcc
     8161 gtggtcatta agggcaagca gaatcgattc gccctcgacg aattcaaacg caacgtttgt
     8221 ggtgttggcc agccttggag cgccttctcc atttatgaaa aggttcggga tttgatcctt
     8281 gattgccttt tcaagcctgt cgcgcatcgc cgtcgagtgg ttcgggtgag tcatcaccag
     8341 cctggcggct gcgccaaggc cgacaatcga tgcgacgttc tcggtgccgg cccgccggcc
     8401 acgctcctga tggccgccaa gctgccaggg cctccacctc gagccgcgcc gcacaaacat
     8461 cgccccaaca cccttcggcg cgtgcagttt gtggcccgaa atcgtcagca gatcgacgtt
     8521 cttcagttcg ccctgcagat ccagcctcat cttgccggcg gtctgggttg catcggtgtg
     8581 aaagaccaca tccggatcga ccttcttgac cagtgccgcg agttccccga tcgggaagat
     8641 gacgccggtt tcgttattgg catgcatgat cgacacgacg gcggtatcgt cctggatggc
     8701 gcggatcaaa tcggccgcgt caagcattcc atgcctgttg accggcaggt acgttgcccg
     8761 gaaaccttcc ctttccattt ccctgacaac ttcaagaacc gcggggtgtt caacggccgt
     8821 ggtgatgaca tgccgtcgtg ccggattggc cctggccgcc ccccagattg ccgaattgtt
     8881 gccttccgtg gccccgccgt tgaatatgat ttccgacttg cttccggccc cgagcaggcg
     8941 tgcaacgctc tcccgggccg cctcgacggc ctcggcagcc ttccttgcct ggggataggt
     9001 cgaactcgga ttgaagaact gctccttaag gaacggaatc atcgcctgca gaacttcgtc
     9061 ggcaaccggc gtggtggcgt tgttatcaag ataaatgatt ctgtcgttca tcattccccc
     9121 gcggacaaat cagatggcca cgatttcaag ctcggcgtcc accttggccc tgagagcgtc
     9181 ttcaacgaca agcctcatgg tctggggcgc gcccttgcat ccctggcaac cgccggtcag
     9241 tcggcagaat acctttttgc ccctgatctc cactatctca agatcgccgc cgtcgtgctg
     9301 aagcctgggc ctgatgtcgc tttcgaccac gcgatcgact tcgcgggcaa gctggaacgg
     9361 cgtccttgtc atggaactgc tggtctgtgg catcggctgc ggatccgtga tggccggggt
     9421 ggctgcctgc gacgaatcgc tgcggcccca tgtgtcatca aggatgtcct ggagcccgcc
     9481 cggttcgtgg tggcaggccg tgcaggcacc acccgccttg atcgcattgg ttatgtccgc
     9541 aatcgttttc aggttcagtt cctttacctt gcgccttatg aatggttcgg tcaggttgaa
     9601 gcagcggcag acgatacggc cttcgtcctc gccggtcggg atggcgtcaa gtccaagctc
     9661 cgacagcgga atgttccgct tgtgcgccca gtcggcaacg gcggcctgca gggcctcggc
     9721 gcccatgacc gagcagtgca gcttctgtgc cggaagcccg tcgaggtaat cgacaatgtc
     9781 ggcattcgtg atcttcaacg cctggatggg tgtgagaccc ttttcctcga tcaacgcgca
     9841 cagcgcttcc gaggatgcga tggccgaggt gcatccgaag gtccggtacc ttgcctggac
     9901 gatcttctgc cgccgcgggt caggttcggt cgcatcgacc ttgaaagcga attcgagcgc
     9961 gtcgccgcag gaaagcgatc cgtgagaccc gatcccatcg ggatcgtcga tggaacccat
    10021 gtgggttccc gctttcgccg ccattgcgtc ccgaaacaat tgaagaactt tttcggagta
    10081 atcccaaggc atgacttttt ccccgcgttc gttttccagc ccgtgaccag gccaggtttc
    10141 aatcacgggt aatttaagtc cgaattaagc cgctgtcaac cgcgccgcgg cttccacacg
    10201 tcaagattcg ggggtaatat acgacggctt tctgggggcc ctgtgcatgg aatcgattgg
    10261 aaccccgctt ctgtgggttg gttttgtcgt tttcgttctg atcatgctcg ctgttgacct
    10321 tggcgtcttc cagcgcaagg cccacagcgt cagcatgaga gaggccggac tctggacggt
    10381 cgtctggatt gctctggcat tgatcttcaa cgttggcgtc tatcactggt ttggatacgc
    10441 ccacgcgatg gagttcctga ccggctacct gatggaaaag gcgttgtcca tggacaacct
    10501 tttcgtgttc ctggtgctct tttcgtattt cggcgtgccc gggcacctgc agcatcgggt
    10561 gcttttcttt ggaatcctgg gcgccctgat catgcgggcc ctgttcatcg tggccggtgt
    10621 cgcgctgatt tcaaagtttc actgggtcat gtacatcttc ggcgcgttcc tggtttttac
    10681 cggcatcaag ctcgtcatga acaagggcgg tgaggttcat cccgagcgca atttcatgct
    10741 tcgcatattc aagaaattcg tgccgaccgt gcccgagtat cgtgggacgc atttcgtcgt
    10801 gcgtgagggc ggccgcttga tggcgacacc gctgctggcc gttctggttg tcatcgaggg
    10861 aaccgacgtc ctgttcgcgg tcgattccat ccctgccgtt ttcggcatca cgcgggatcc
    10921 cttcatcgtt ttcacctcca acatcttcgc gattctgggg cttcgcgccc tgtatttcct
    10981 gcttgccggc atcgtcgaga agttccgttt cctgaagtac gggctgggac tggtgctggc
    11041 attcgtcgga ttcaagatgc tggtggagga tttgcttcac cacatgcacc gggaaatccc
    11101 gatcgccatg tcgcttgcgg tgattgccgg cacggtcggc ttgtccatcc tgatttccct
    11161 gatcattcca ccgaaggccg attcgaggcc gggcgatcag tccgccgggc agtgataccg
    11221 gttacacgcg tttcgttact cctcacgccg cccgtcccgg gtgcaaccgt acgctcgacg
    11281 agagacgcag tcgtgcctgt ggccggccat gaagtgtccg gttcgagggg cctcaccgga
    11341 gcacttacgg atcggcccga tccgccccgc atctgttctc gtacgaactg gtcgtccgcg
    11401 atatatcctt aatatcattg tctttttatt ggggtaaatc ccggcgcgtt gatgacttgt
    11461 caattttcct gcatggcccg ataatggttc agaagtcaaa gactccctcc ggtttgtcac
    11521 cggtgcgcga gacagcaatg cggtcttcaa actggccggg ttcgtccgac gtcgcgtcga
    11581 tgaacccctt tgctgttggt gccggatgtt tccggcaatt gcgcggtgaa tcatcatgaa
    11641 agaagaattc ggcggttcaa acaggtcttc ggtcgaggcc cgtggcggga ggctcgcctc
    11701 gatggatatc agggg
//
LOCUS       DAOPPA010000035        11586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6419_length_11586_cov_4.127482, whole genome shotgun sequence.
ACCESSION   DAOPPA010000035 DAOPPA010000000
VERSION     DAOPPA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6419_length_11586_cov_4.127482"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKH54_02015"
     CDS             complement(<1..444)
                     /locus_tag="PKH54_02015"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOC98690.1"
                     /translation="MESLLLSFGSLKMEWYNAVIALGLSFLLASILAVVYEKTFRGLS
                     WSSGLVQSMILGPMVSCLIMIAIGDNVARGIGIVGSLAIIRFRTNLRDPRDLIFLFAA
                     LGIGVACGVRSYTTAIAGTLLFCTAAVVLQFTRFGQRRNHDGMVRF"
     gene            578..1249
                     /locus_tag="PKH54_02020"
     CDS             578..1249
                     /locus_tag="PKH54_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013373930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC98691.1"
                     /translation="MSERIVLIEDDRELGAQIVAHLSRAGYQVELKTEGTFPIPADFQ
                     GVSLVILDLMLPGLGGMDILKHLRTFSDVPVMVLSARNNTSDKVRALKMGSDDYMTKP
                     FWPEELVERVRARLRRPNLQKTGPLTVAGVCLDASRRTATAGGAGIELTRVEFDILAS
                     LMRRPGEAVTRQWLVANVLDPDKDSSERTLDVHVSHLRRKLGKENIIETVWGIGYRAG
                     KGGSR"
     gene            1246..2886
                     /locus_tag="PKH54_02025"
     CDS             1246..2886
                     /locus_tag="PKH54_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC98692.1"
                     /translation="MKLRAWLTLATAATGIAVFAGAWAGFGYYRDFTRREILVQSTLS
                     YMTAESRAECEKNAASWMPEPARTAPGKDWVNGRRPPMRRRLFAYDSAFVSANVNAPE
                     IDSDMMKAAAAGQIAWKSWTGVSRMGPPWRKDGPGGGDLRPDLERDAGEPGAPPDQPL
                     VRLGRILVPMPWKTGPCANVLVEYEEPPESAGPGGFTPPTVVWIIPVLFMIIAVLVAA
                     GPVVGRIRRLTRMVRQSALAGYEKPIFLKGSDEIAELGAAFETARTEILGRIALQERR
                     EQNLREFLENTTHDISIPLTVLQGRLADLAGDAERGGADPDKVGGAISEAQYIAALIG
                     NLAVASRIQTGQPEPQKEPVDMCAVVQRCVARHRPIARQARVTLEHAVPEHVVFARGD
                     VTFIEQAVNNLVFNAIRHNRPGGHVAVILEEPGNGLPMSANRQGAVETMPRETPPASI
                     CVIDDGPGIPEEELSRLAERYYRAGRDRGRNAGGQGIGLHIASTVAAIHGWRFSLSKS
                     EYGGLLARLEFEMDGPDDRPDDDPVIKRDNQKTDAGVH"
     gene            complement(2883..4988)
                     /locus_tag="PKH54_02030"
     CDS             complement(2883..4988)
                     /locus_tag="PKH54_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98693.1"
                     /translation="MNKAFVNDRFRDDPAGYPSRQKSPGPSASLAVVLILGAFSACNE
                     LPVHEVEPIDCSFRVIEHTFPDELNDISTGDWTVPAYEHASILLDEKYAHQLNFMMSP
                     MMPPYMKPLASNGPIILWSDRFETMVFSPLNAFGSSWMKAEDGKLRYGMHGDTDRVPA
                     GTVQRFLIVEGEGVNHSVDCWGRLMRRDSGVASPDRYADTGVSTLGYWTDNGAAYYYQ
                     TEGDMNEQDTLLAVKAEAASLGIPYGYFQIDSWWYFKEAQSGGLGGMLSGGVLEWKPM
                     PEMFPDGLAAFRDQLGLPMIAHNRWVSPLSPYLQKYDFVMDDPEAEMAIPTGPELFRE
                     FMQSCLDWGIETYEQDWLVSQYFGSRWLRNEPGHLEQWLSNMHGAAAEAGLTTQICMT
                     GAAHLMQAASHNNVTTVRTTIDYTKDVCKECYWPQFHINNMVAGALGVWPFKDNFQSS
                     ELHGQAEALVSSLSAGMVGVSDAIGKTVKDFVLATCRQDGVLLKPDRPATPIDPMFTP
                     NERPFTVSTVSTVKGVGEWTYIAAFHIASAHPKRSDEDRLWEILLYDDVGVANMFNYP
                     ETVTDWSFDMLRDIGQVGERVLYNWRTGTAVAIEDTFDLPEVEGHNDFTYLVLAPVSE
                     NGLALIGEPAKYVTMADKRMIGVKVQKNGFKVTVAGKPGERVELAAFDTRVSRMLEPG
                     IGTIGADGTAVITIGRALD"
     gene            5189..6985
                     /locus_tag="PKH54_02035"
     CDS             5189..6985
                     /locus_tag="PKH54_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98694.1"
                     /translation="MKMTAFPTVAACFLSACAIALAGCGAGGDDKDTGTDLGPEIGQP
                     DTVDDDGPGDTAMDAADPETTGDEGSKPDLIMDAETVDPDDARDDAADAFDATPDGLP
                     ETLALEFTRPDEGDPVSQADIDAFTRKVMRFLRDVKYFDYVLYTTHGVDASTGKRDFQ
                     FWYNERMRKEGDLVTFYHPVNMTDGGHNLHIPMSKLLGNLMAAYMLTGDATAGLAVEK
                     LCKGFSASMLGMVHDENDDLPHLMTRNVVMMAHEFTTHDGKRKAVDPSGWYSAYERWN
                     CDRYQITENPYWGEVWVTNLRSKDDVPHILQMIPNLRYLVETAPAGATLDACTETLTL
                     LEAFTKDIVDHDYRIRTKDFDGTLYEPGYTGDAETDQNQGDIGSFTYWRDFIPMAECN
                     ARRASMLIAYKKPGEESCGRGEPNDYDDIAFMNNGYNKRICQFFHLAHIANALVNHDN
                     GEAALLLDGMAERIAEDWALAPNQDKIQVDDYTRNVALLAAQASVFGYPLTSAEVRLI
                     HTYYGRTPDKIADWPYWDPWAETVPQGDLGAYRPPDCEGEGDAKLCWTRIEDMGALWE
                     YCWSPLKNPAGRQWVNCDIVRDPETWDEDLPD"
     gene            complement(7150..7848)
                     /locus_tag="PKH54_02040"
     CDS             complement(7150..7848)
                     /locus_tag="PKH54_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC98695.1"
                     /translation="MRSWTTTDGTRIFLVCGGRSNVYLVANETASFLVDAGMFAAAPL
                     VTHGIARTGAPAPSYLVLTHTHFDHAGATASLRRRFNLKTIVCDHDVKFLGCGDSPFP
                     EGNTRAIRALMALIRRPGELMMQYPPAVTDIPVRGRLDMGPLGFDGTEVIHTPGHSMG
                     SVSVVVDKEIAMVGDTLYGLALRSVMPPLVDEPIKLFFSLCALRDTGARLFLPGHGPA
                     IPRERLEKLIDELS"
     gene            8028..8681
                     /locus_tag="PKH54_02045"
     CDS             8028..8681
                     /locus_tag="PKH54_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98696.1"
                     /translation="MKGKLGISALLVMCSITLVASTASADWGGAHGRRSSSSGVSKPK
                     AYVGVTGNMLFYPEVIISGDMNGSGMAAFSGGLSFILEGYPNKYLAAGLLLDVNFQDY
                     KNVPSVDWVEPQISLDISLRPTVPLGSKGRFELYFRLAVGYTAFIPSRAVMDAGATLD
                     DSEHGWNVKLLPGFAVRVARGLTLNAELGWVGCGFLAAGSHVFIHSPALNFGIGYTF"
     gene            8788..9978
                     /locus_tag="PKH54_02050"
     CDS             8788..9978
                     /locus_tag="PKH54_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98697.1"
                     /translation="MYERPILKNLLDRLAEPRRFIQVLAGPRQVGKTTLARQAMAAIG
                     DNVIYASADEPILKGATWVEQQWQIARIRAAGTQSGAVLILDEIQKIPDWSAVIKRLW
                     DQDSADKTQIRLVLLGSSPLLVRQGLSESLAGRFEMIPVTHWSWPEMRDAFGWNLETW
                     ISFGGYPGAAPLIGTPDRWAAYIMDSLVETSVSRDILQMTRVDKPDLLRRLFDLGCAY
                     SGQVLSYQKMVGQLQDAGNTTTLSHYLTLLEQAGLLRGLNKYAGEKFRQRASSPKLLA
                     LNNALVVTARGIRTGAIREDHETWGRLVESAAGAHLSNGPWVAGASALYYWASGDREV
                     DYVLAGSGKIVAFEIKSTRRRAAVPGIDFFEKEFGPVRKILVGGQGLPLEEFFTMEPA
                     DWLA"
     gene            complement(9982..11364)
                     /locus_tag="PKH54_02055"
     CDS             complement(9982..11364)
                     /locus_tag="PKH54_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98698.1"
                     /translation="MQDTDSMNLQARVWRAVEFSADPGALAMALDHFMPQCSFGVSDE
                     VPTARISRAGDKMAIEFGRAFLARELSDDRDFLFVLMHEIYHNVLGHMCTSRGDRVSR
                     IYHNLSNIAADMMVNRAVCNRFFPGGVPLLERMYGRESLPGALLVPPEFDLSRTPEAQ
                     ENRKIMLTRFARGLKRLGASPRLAIRVWGIYRQAWFKDAPYEHVLERLLNLFGAIDPL
                     ALPPIILIGSHDGPFGGLGDILGMEGDEKGPAGQGGDIEEEMVEVDKTVSCLGVAAAL
                     RRALETNSNRWRMQDPVACPGVVCTPGRRDAAFLAMGSFPVFYSANKVEYEPEQLAHV
                     YVDVSGSMVREIPHMFGVLAQSRELIADPIHEFSNVIEDVTMHEFSQGVCRTTGGTDF
                     TVLLEHAIKNRFRQIVVFTDGYATSDQAVVERFKAAGRKLHLVFVDSCAHGRAECDLL
                     PLATSVFVMS"
     gene            complement(11391..>11586)
                     /locus_tag="PKH54_02060"
     CDS             complement(11391..>11586)
                     /locus_tag="PKH54_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98699.1"
                     /translation="RDILCRSSRGYVIPGLGADVLPPSFKKLAGRWLALCVPVQWIGA
                     SGGIGPEPPGPVSADLISAA"
BASE COUNT         2447 a         3492 c         3484 g         2163 t
ORIGIN      
        1 gaagcgcacc atgccatcat ggttcctgcg ctggccgaac ctggtgaact gcaggactac
       61 ggcggcagtg cagaacagca gcgtgccggc aatcgcggtg gtatagctgc gcactccgca
      121 ggcaacgccg atgcccaggg ccgcgaacag gaaaatcagg tcgcgcgggt cgcgaaggtt
      181 cgtcctgaag cgaatgattg caagcgagcc gacaataccg atgccgcggg cgacgttgtc
      241 gccgatcgcg atcatgatca ggcaggaaac catcggtccc aggatcatcg actgaaccag
      301 gccgctggac caggacaggc cgcgaaaggt cttttcatat acgacggcca ggatgctggc
      361 cagaaggaag gaaagcccaa gcgcgatgac cgcgttgtac cattccatct tgagcgaccc
      421 gaaactcaga agcaggcttt ccatgtctta tccttcagcg tcgcggcaat ttatcaattc
      481 attcttaaga ttatcttaat cctgacccaa tataatgggc cagttcacgc ccgtgcggtg
      541 acgggccatt attgtacggc aatccggggg caagtccatg tcggaacgga tagtgcttat
      601 cgaagacgac agggaactgg gcgcgcagat cgtggcgcac ctttcccggg ccggttacca
      661 ggtcgagttg aagaccgagg gtacattccc gattcccgcc gatttccagg gcgtctcgct
      721 ggtgatactg gatctgatgc ttccgggcct gggaggcatg gatatcctga aacatctgag
      781 aaccttttca gacgttccgg tgatggtgct gagcgcgcga aacaacactt ccgacaaggt
      841 gcgggcactg aaaatgggct ccgacgacta catgacgaaa ccgttctggc ccgaggaact
      901 tgttgaacgg gttcgcgcca ggcttcgcag gccgaacctt cagaagacgg ggccgctgac
      961 ggtggccggg gtctgcctgg atgcctcgcg acgaacggcg acggcgggcg gggctggtat
     1021 tgagctgacg cgggtcgaat tcgacatact tgcatcgctg atgcggcgtc cgggtgaggc
     1081 cgtgacaagg caatggctgg tggcaaacgt acttgacccg gacaaggaca gttcggaaag
     1141 aacgcttgac gttcacgtat cccacctgcg caggaagctc gggaaggaaa atatcatcga
     1201 aaccgtttgg ggcatcggct accgtgccgg caaaggcggg tcacgatgaa attgcgagcc
     1261 tggttgacac ttgccacggc ggcgacaggg atcgccgtat tcgccggcgc ctgggcgggt
     1321 ttcgggtact accgcgattt caccaggcgg gagatcctcg ttcaatcgac actctcatac
     1381 atgacggccg aatccagggc cgaatgcgaa aagaacgccg cgtcctggat gcccgaaccc
     1441 gcaagaacag ctcccggaaa ggattgggtc aatggccggc ggcccccgat gagaagacgc
     1501 cttttcgcgt acgacagcgc attcgtttcg gccaacgtca atgcgccgga aatcgattcc
     1561 gacatgatga aggcggccgc ggcgggtcaa attgcctgga aatcgtggac gggcgtctcc
     1621 cgaatgggac cgccgtggag gaaggacggg cctggtggcg gggatcttcg cccggatctt
     1681 gaacgggacg ctggtgaacc gggcgccccg cctgaccagc cgcttgtcag gcttggcagg
     1741 atccttgttc cgatgccgtg gaaaaccggg ccctgcgcga atgtgcttgt cgagtatgaa
     1801 gagccccccg aatcggcggg acctggcggg ttcacacccc cgacagtggt ctggataatc
     1861 ccggtactgt tcatgatcat tgccgtactt gtggcggccg ggcctgtcgt cggaaggatc
     1921 cggagactga ccaggatggt tcgtcagtcg gcactggccg ggtacgaaaa accaatcttc
     1981 ctgaaaggct ccgacgaaat cgccgaactg ggcgcggcct ttgaaacggc tcgcaccgaa
     2041 atcctgggcc ggattgccct gcaagaaaga cgggaacaga acctgcgtga attcctggaa
     2101 aacacgacac atgacatcag catacccttg acagtcctgc agggccgcct tgcggatctg
     2161 gcgggtgatg ccgaacgggg cggggcagac cccgacaagg tcggcggcgc aatctcggag
     2221 gcccagtata ttgccgcact tatcggtaat ctcgcggttg cctcgagaat ccagacaggt
     2281 caaccggagc cacagaagga accggtcgat atgtgcgccg tggtgcaaag atgcgttgcc
     2341 agacaccgac cgattgccag gcaggccagg gtaaccctgg aacacgccgt tcctgaacat
     2401 gttgtcttcg caaggggtga tgtgaccttc atcgaacagg ccgtcaacaa ccttgtcttc
     2461 aatgcaatcc ggcacaacag acctggcgga cacgtcgccg tcatacttga ggaacccggg
     2521 aatggattgc ccatgtccgc gaatcggcag ggtgccgtgg agacgatgcc acgggaaacc
     2581 cctcctgcat cgatatgcgt gattgatgac ggccctggaa tcccggagga agaactttcc
     2641 cggctggccg aaagatacta ccgggcgggg cgggatcgcg gacgcaacgc tggcgggcag
     2701 ggcatcgggc ttcacatcgc ctcaaccgtc gcagcgatcc acggatggcg tttttcgctt
     2761 tcgaaatccg aatacggtgg acttctggcc aggctggaat tcgaaatgga tgggccggac
     2821 gacaggccgg acgacgatcc ggtaatcaag cgggacaatc agaaaaccga tgccggagtg
     2881 cattagtcga gcgcgcggcc gatcgtgatg accgctgttc cgtcggctcc tatcgtgccg
     2941 attccgggct caagcattct tgaaaccctg gtgtcgaaag ccgccagttc gactcgctcg
     3001 cccggtttgc ccgcaacggt caccttgaaa ccgttcttct gaaccttcac accgatcatc
     3061 cgcttgtcgg ccatcgtcac gtacttcgcc ggttccccga tcagcgcgag cccgttttca
     3121 gacaccggcg caagcacaag gtaggtgaag tcgttatggc cttcgacctc ggggaggtcg
     3181 aacgtgtctt caatggcgac cgccgtgccg gttcgccagt tgtacaggac gcgctcgccg
     3241 acctggccga tgtcgcgcaa catgtcgaac gaccagtcgg tgacggtttc cggataattg
     3301 aacatgttcg cgacgccgac gtcgtcgtaa agcaggattt cccacaggcg atcttcgtca
     3361 gaccgcttcg gatgggcact tgcaatgtgg aaggcggcaa tgtacgtcca ttcgccgaca
     3421 cccttcacgg tcgaaactgt cgaaacggtg aaggggcgct cgttcggggt gaacatcggg
     3481 tcaatcgggg tcgccggccg atccggcttc agcaggacgc catcctgacg gcatgttgcc
     3541 aggacgaaat ccttcaccgt ctttccaatc gcgtcgctga cgccgaccat gccggccgaa
     3601 agggagctga caagggcttc tgcctgaccg tgaagctcgc tgctttggaa attgtccttg
     3661 aacggccaga caccaagggc gccggcgacc atgttgttga tgtggaactg cggccagtag
     3721 cactccttgc agacgtcctt cgtgtaatcg atggtcgtgc ggacggtcgt gacgttgttg
     3781 tgggaagcgg cctgcatcag gtgtgccgcg ccggtcatgc agatctgcgt ggtcaggccg
     3841 gcctcggccg cggcgccatg catgttcgaa agccactgct ccagatggcc cggttcgttg
     3901 cgcagccatc ggcttccgaa gtactggctg accagccagt cctgctcata cgtctcgata
     3961 ccccagtcca ggcatgactg catgaattcc cgaaaaagtt ccgggccggt cgggatggcc
     4021 atttcggctt caggatcgtc catcacgaag tcgtacttct gcagataagg gctgagcggc
     4081 gaaacccagc ggttatgggc gatcatcggc aggccaagct ggtcgcgaaa ggcggcaaga
     4141 ccgtcgggaa acatctcggg catcggcttc cactcaagaa cgccacctga aagcatgcca
     4201 ccaagcccgc cgctctgggc ttccttgaaa taccaccaac tgtcgatctg gaaatatccg
     4261 tatggaatgc caagcgaggc ggcctcggcc ttcacggcaa gcaaggtgtc ctgctcgttc
     4321 atgtcgcctt cggtctggta atagtatgcc gcgccgttgt ccgtccagta tcccagggtc
     4381 gaaacaccgg tatcggcata ccgatctggg gacgccacac cgctgtcgcg gcgcatcaac
     4441 cttccccagc agtcaaccga atggttcacg ccttcgcctt cgacaatcag gaaacgctgc
     4501 accgtgccgg cggggacgcg atccgtgtcg ccatgcatgc catacctgag cttgccgtct
     4561 tcggccttca tccaggaact gccaaaggcg ttcagcggcg aaaaaaccat tgtttcgaac
     4621 cggtcgctcc acaggatgat cggtccgttg ctggccagtg gcttcatgta cggcggcatc
     4681 atcggggaca tcatgaagtt caactggtgc gcatattttt cgtccagcag gatggatgcg
     4741 tgttcataag ccgggacggt ccagtcgccg gtcgagatgt cgttaagctc gtccgggaag
     4801 gtgtgctcga tcacgcggaa actgcagtcg atcggctcaa cctcgtgcac cgggagttcg
     4861 ttgcacgccg aaaaagcacc cagaatcaac acgaccgcca gggaagccga cggaccgggg
     4921 gatttctggc gagatgggta cccggcaggg tcatcccgga aacggtcatt cacaaacgcc
     4981 ttgttcatat tgattccccg tggcatgaac tgtcaccggc cagatctaac cattggattt
     5041 cacgcaaatc aacaatgtcg agccttgccg ggcgtgggca ttcgcggcga tgcccggggc
     5101 cgaccgtgcg gccgacagtg cggctgccct tgacccgggg gcctttgaac tgcgatagtc
     5161 accgtgtcaa caaccggggt tcataagcat gaagatgacc gcctttccga ctgtcgcggc
     5221 ctgtttcctg tctgcctgtg cgatagccct tgccggatgc ggcgccgggg gggatgacaa
     5281 agacaccggc accgacctgg gccccgaaat cggccagcct gacacggttg atgacgacgg
     5341 accgggggac accgcgatgg acgcggccga tcccgaaacg acgggcgacg agggttcaaa
     5401 gccggatttg atcatggacg cggaaacagt tgatccggat gacgccaggg atgacgctgc
     5461 tgacgcgttc gacgccacgc ctgacggact gcccgaaacg cttgcgcttg aattcacccg
     5521 acctgacgaa ggcgatcccg tttcccaggc cgatattgat gcgttcaccc gcaaagtcat
     5581 gcgtttcctg cgcgatgtga agtacttcga ctatgtgctt tacacgaccc acggcgtcga
     5641 tgcatcgacc ggcaagcgcg acttccagtt ctggtacaac gaacggatgc gcaaggaagg
     5701 cgacctggtg accttctatc acccggtcaa catgacggac ggcggccaca acctgcacat
     5761 cccgatgagc aagctgcttg gcaacctgat ggccgcgtac atgctgacag gggatgccac
     5821 cgcgggcctg gcggtggaaa agctgtgcaa gggcttttcg gcatcgatgc tgggaatggt
     5881 tcatgacgag aacgacgacc tgccccacct gatgacccgc aacgtggtca tgatggcaca
     5941 cgaattcacg acgcacgacg gcaagcggaa ggcggttgac ccgtccgggt ggtactcggc
     6001 atacgagcgc tggaactgcg accgttacca gatcaccgaa aacccgtact ggggcgaagt
     6061 ctgggtcacg aaccttcgat ccaaggacga cgtcccgcac attctgcaga tgatcccgaa
     6121 cctgcggtac cttgtggaaa cggcaccggc cggggcaacc ctggacgcat gcaccgaaac
     6181 gcttaccctg ctggaagctt tcacaaagga catcgttgac cacgactacc gcatccggac
     6241 aaaggacttc gacggaaccc tatatgaacc cggatacacc ggtgacgcgg aaaccgatca
     6301 gaaccagggc gacatcgggt cgttcacgta ctggcgcgac ttcatcccga tggccgaatg
     6361 caacgccagg cgcgcatcga tgctgatcgc gtacaagaag ccgggcgaag aaagctgcgg
     6421 ccgtggcgag ccgaacgatt acgacgatat cgcgttcatg aacaatgggt ataacaagcg
     6481 aatctgccag ttcttccacc ttgcccatat cgcgaacgcg ctggtcaacc atgacaatgg
     6541 cgaagccgcc ctgctgctgg acggcatggc cgaacgcatc gcggaagact gggcactggc
     6601 gccgaaccag gacaagatcc aggtggacga ctatacccgc aacgtggcgc tgctggcggc
     6661 ccaggcgtcc gtgttcggtt atccgcttac atcggccgag gtcaggttga tccacaccta
     6721 ttatggtcgc acgccggaca aaattgccga ctggccttac tgggatccgt gggcggaaac
     6781 ggtgccgcaa ggcgacctgg gcgcttaccg gccaccggac tgtgaaggcg aaggggatgc
     6841 aaagctctgc tggaccagga tcgaggacat gggggctttg tgggaatact gctggtcacc
     6901 gctgaagaac ccggccggac gccagtgggt caactgcgac atcgtacgcg acccggaaac
     6961 ctgggacgaa gacctgccgg attgatggcc gggacgggct ggccgggccg ggctggccgg
     7021 gccacggccc acgctgcctg caacttcaag gctttgcagg ttgtatatgg gattacatca
     7081 gccccggaac actacagccc tgggcccctg ctgctctgcg ggttgtatgt ggggttacat
     7141 ccgggcgctt caactcaact catctatcag tttttcaagc cgctcccttg ggatggccgg
     7201 gccgtgtccg ggaagaaaaa gacgggcgcc ggtgtcccgc agggcacaca ggctgaaaaa
     7261 cagctttatc ggttcgtcca ccaacggcgg catcacggat ctgagcgcca ggccataaag
     7321 cgtgtccccg accatggcga tctctttatc gacaaccacg ctgacagacc ccatcgaatg
     7381 gccgggcgta tggatgactt cggttccgtc aaatcccagc ggccccatgt ccaggcgtcc
     7441 cctgactggg atgtcggtca ccgcgggcgg atactgcatc atcaactctc cgggacggcg
     7501 gattaacgcc atcaatgccc tgatggcccg ggtgttacct tcgggaaagg ggctgtcacc
     7561 gcaacccagg aacttcacgt cgtgatcgca gactatcgtc ttcaggttga acctgcgacg
     7621 aagcgaagct gtcgcgccgg cgtggtcaaa atgcgtatgc gtcagcacca ggtacgaagg
     7681 cgccggggcc ccggtcctgg cgatgccgtg ggtaaccagg ggggccgccg cgaacatccc
     7741 ggcgtcaacc aggaaagatg ccgtttcatt cgcgaccaga tagacgttgc ttcgcccgcc
     7801 gcagaccaga aagattcgcg taccgtcggt cgtcgtccaa cttctcactg ttccgaagcc
     7861 cccggaatca cgacgacata tttatatccg aacgaaaggc cgtgttcacg aaaaaacagc
     7921 gcggcgcgtc atcgaatgtc ggaattggac ggcccgggat gacgcccgcg caaattttgt
     7981 atatcattgg cctgacttcc aaatccggat atccgacagg ggttgccatg aagggaaaac
     8041 ttggaatatc ggcattactg gtaatgtgtt caattaccct ggtcgcgtca accgccagcg
     8101 ccgactgggg cggagcacac gggcgaagaa gctccagcag cggcgtcagc aagccaaagg
     8161 catacgtcgg cgtaacggga aacatgctct tttacccgga agtgatcata tcgggcgaca
     8221 tgaacggatc cgggatggcc gcgttttccg gcggcctttc cttcatcctt gaaggatatc
     8281 ccaacaagta cctggcggcc ggactgctgc tggacgtcaa cttccaggac tacaagaacg
     8341 tccccagcgt tgactgggtc gaaccacaga taagcctgga catctcgctg cgcccgacag
     8401 tcccgcttgg atccaagggc aggttcgagc tgtatttcag gctggccgtc ggctacaccg
     8461 cattcattcc cagccgcgcc gtgatggacg cgggcgccac ccttgacgac agcgaacacg
     8521 gctggaacgt caagctcctg ccgggattcg ccgttcgcgt ggcgcgcgga ctgaccctga
     8581 acgccgaact gggctgggtc ggctgcggct tcctggccgc cggaagtcac gttttcatcc
     8641 attcccccgc ccttaacttc ggcatcggat atacgttcta ggccagccga tcaatcgata
     8701 acatcccgaa cagactgaat atcttgattt actcaatgta ttgagtaaaa ttactcatcg
     8761 tcttgagcaa ttccacggag ggccctgatg tacgaacgcc ccatattgaa gaatctgctt
     8821 gatcgcctgg ccgagccccg ccgcttcata caggttctgg caggcccccg gcaggtcgga
     8881 aagacgacgt tggcccgtca ggcgatggcg gccatcgggg acaacgtcat ttatgcgtcg
     8941 gccgacgaac cgatcctgaa aggcgcgacc tgggtcgaac agcagtggca gatcgccagg
     9001 atccgggcgg ccgggacgca atccggcgcc gttcttatcc tggacgaaat ccagaagatt
     9061 cccgactggt ccgccgtcat caagcggctg tgggatcagg actctgccga taaaacgcag
     9121 atccgcctgg tgctgctggg atcttcaccg ctgctggtaa ggcagggatt gtctgaaagc
     9181 ctggcgggcc gttttgagat gattccggtt acccactggt cctggcccga aatgcgcgac
     9241 gccttcggat ggaatctgga aacgtggatt tcattcggcg ggtatccggg ggccgcgccg
     9301 cttatcggca ctccggacag gtgggccgca tacatcatgg attcgcttgt cgaaacttcc
     9361 gtgtcgcgcg acatcctgca gatgacccgc gtggacaaac cggatcttct gcggcggctc
     9421 ttcgaccttg ggtgtgccta ctccggacag gtgctgtctt accagaagat ggtcggccag
     9481 cttcaggacg ccggaaacac gacgacgctg agccattacc tgacactgct ggaacaggcc
     9541 ggccttcttc gcggactgaa caagtatgcg ggcgagaagt tccgccagcg ggcaagcagt
     9601 ccaaaactgc tggccctgaa caacgccctg gtcgtcacgg cccgcgggat acgcaccggg
     9661 gccatccgcg aagaccacga gacctggggg cgactggttg aatcggccgc cggcgcacac
     9721 ctgtcgaacg gcccgtgggt ggccggggcg tcggccctgt actactgggc ttcaggcgat
     9781 cgcgaggtcg actacgtcct ggccggatct gggaagatcg tcgcctttga aatcaagagc
     9841 acccgccgcc gcgcggccgt tccgggaatc gacttcttcg aaaaggaatt cggtccggtc
     9901 cgcaagattc tggtcggcgg ccagggcctg ccccttgaag aattcttcac catggagccg
     9961 gccgactggc tggcatgacc atcacgacat cacgaagacg ctggtggcca ggggcagaag
    10021 gtcacattcg gcgcggccat gggcgcatga atcaacgaat accagatgca gttttctgcc
    10081 ggccgccttg aagcgttcga ccacggcctg atccgatgtg gcatagccgt cggtgaacac
    10141 cactatctgc cgaaacctgt ttttgatcgc atgttccaga agcacggtga aatcggtgcc
    10201 cccggttgtc ctgcagacgc cctggctgaa ttcgtgcatc gtcacgtctt cgatcacatt
    10261 tgaaaattca tgtatcggat ccgcaatcaa ttcgcgagat tgcgccaaca ccccgaacat
    10321 gtggggaatt tcccgcacca tcgaacccga cacatcgaca tagacgtggg caagctgctc
    10381 cggttcgtat tcgaccttgt tcgccgagta gaacaccgga aaacttccca tcgccaggaa
    10441 cgccgcgtcc cttcggcccg gggtgcagac cacgcccgga catgcaaccg gatcctgcat
    10501 gcgccagcga ttcgaattgg tttcaagggc acggcgcagg gcggcggcca cgcccagaca
    10561 ggaaaccgtc ttatcgacct cgaccatctc ctcttcaatg tccccaccct ggcccgccgg
    10621 acctttctcg tccccttcca tccccaggat gtccccgagc cccccaaaag gcccgtcatg
    10681 actgccgatc aggatgatgg gcggcaacgc cagtgggtca atggccccaa acagattaag
    10741 aagccgctcg agaacgtgtt catacggggc atccttgaac caggcctgcc tgtagatgcc
    10801 ccacacccgt attgccaggc gcgggctcgc gccaagcctc ttcaggcccc gcgcgaatct
    10861 ggttaacatt attttccggt tttcctgggc ctcgggcgtg cgcgacaggt cgaattccgg
    10921 cggaacaagc aacgcacccg gaaggctttc gcgaccgtac atccgttcca gcaggggcac
    10981 gccgcccgga aagaacctgt tgcagacggc acgattcacc atcatgtcgg ccgcgatgtt
    11041 ggacaggttg tgataaatac gcgagaccct gtcaccacgg cttgtgcaca tatgtcccag
    11101 cacgttgtga tagatctcgt gcatcaggac gaaaaggaag tccctgtcgt cagacagttc
    11161 gcgggccaga aaggcgcggc cgaactcgat tgccatttta tcgccggcac gactgatacg
    11221 cgcggtcgga acttcgtcgc tgacgccgaa actgcactgc ggcatgaaat ggtccagcgc
    11281 catcgccagc gcccccgggt cggccgaaaa ctcgaccgcc cgccacacgc gcgcctgaag
    11341 gttcatggaa tcggtgtcct gcatttgtcc ccctttttca aaagcccttt tcacgcagca
    11401 ctgatcagat cggcagacac cggtcccggc ggttccggcc cgatgccgcc ggaggcccca
    11461 atccattgaa ccggcacgca gagcgccagc cacctgccgg ccagtttctt gaacgagggt
    11521 ggcaggacgt ccgcacccag gcctggaatc acatagccac gcgatgaacg gcacaggatg
    11581 tcccgg
//
LOCUS       DAOPPA010000036        11542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6442_length_11542_cov_3.903717, whole genome shotgun sequence.
ACCESSION   DAOPPA010000036 DAOPPA010000000
VERSION     DAOPPA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_11542_cov_3.903717"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PKH54_02065"
     CDS             <1..1114
                     /locus_tag="PKH54_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOC98700.1"
                     /translation="HPAQAVSFMNRCVAEDDCLRGVFLVGDLPSAWYEQDSDYGGDIG
                     IMHEEFPTELFFQDLDNQWRDDDGNGILDGHSDFALEIFSARLVGGVERMKSYFARLH
                     EYRQNGSFFNPRNFFSFIDDDWNGANSYGVPDGSTTNQEWGLESVYGDNYIRREWSDN
                     TDKTDYLEVMSGGGAEFVYQWIHSDPQQIYFDDNFSPNPDNILSLQELVAADVSGSFF
                     NLFDCSISRYTEPMGNIATEYVYSRYGLATVGSTKTGGIFNPDVLHGAMLAGHGTGQA
                     LRMWFDDTWANRDAYGLPDPTFDDWWLGMMIQGDPLVLLNPQAGSSLPRSVSATTGPK
                     SERVFELREIDALYRIMGRRAATTVVNGHREYMRNR"
     gene            complement(1119..2522)
                     /locus_tag="PKH54_02070"
     CDS             complement(1119..2522)
                     /locus_tag="PKH54_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC98701.1"
                     /translation="MNDPRKPEKTELYGILTTTTATFLTSFAGSSSNIALPTIGAHFG
                     LDAVDLGWIALSYMLATAALVLPMGRLGDIHGRRRVFIIGVTIFTVASALCALPFDFG
                     FLLAARVLQGVGASMQFATGMAMTTSIFPAARRGAALGINTASVYVGLSVGPTFGGWL
                     THAFGWHSVFWACVPVGAVVLFISALLLKGEWKDAEGESFDLAGSLVLGAGLVCLLLG
                     LTWITRPAGLALIGCAGALFLLLAFIENRVPQPILNMRLFYGNRAFTFSNIAALINYM
                     ATFGVGFLLSLYLQNIRGLTPQAAGTILLTQPIVQAVFSPLAGRLSDRVEPTILASLG
                     MAVTACGLIALSFVTIGTGLPMIVACLMTLGAGFALFSSPNTNAVMRSLPRKWFGIGS
                     ATLSTMRIVGQILSLGIATVMFGVMLGNARIDQTVRTEFMEGISLTFRIFGGLCLVGI
                     IFSMNRGNVLTGKGNLD"
     gene            complement(2561..3946)
                     /locus_tag="PKH54_02075"
     CDS             complement(2561..3946)
                     /locus_tag="PKH54_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98702.1"
                     /translation="MKSLSVPVVLLTFLMAVGCGSGNGDNDIGEGDVVIDTDVSLPDT
                     GGPDSITDVERDVIAPDNGGQDTGLPDEGTPDAADASVDQSQGDTPPDGIEDADEDAA
                     TPPENPWKMMSIRIEDEVVRGTSIMNPVDGMTFGFNPVTDEYLTQFTHDWKDPLISYA
                     WHLDESSGVHTRKVLEGSPWADGTNFCMDEDWCQFIAWDAGAGEWIVMGPRSPGLMRI
                     DTSWRATPVPLAAGDDGQPPNGGISYSHLFIGDAFFVYGWITGSGFSDTLLKFELGTA
                     DWSTVLPGLLPVFSNCLAFDPVENVLISVGGLTTEDGGETSYPLDRIQIIDLDGPSAS
                     TLDLPEGLLGREGFSCAIDGATGHLYVFGGAKIEDNWDERLNTYHNDLWSWDGTNWTN
                     LIPDGPTGTFRQYGESWSLVGDKTLPNFGKNIGRMVYDENSSKPRLILMGDVPGTATQ
                     IYTLTLSDIGL"
     gene            complement(4054..4716)
                     /locus_tag="PKH54_02080"
     CDS             complement(4054..4716)
                     /locus_tag="PKH54_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98703.1"
                     /translation="MHRYLPEISFVIALSMAAFGGAGCEKTIDGAKSVLKAEAGSSSE
                     AANASRPRVNSVANMGTAVIPPGWCSVKFDPGRGPKLALPETEMFHQGPAMVTGRPRW
                     INMWATWCKPCIAEIPHILSWREARLGTGTDISLELLSVDEDLKTLQAFLARRPDLRT
                     VKLQRMTNPGSLGEFAQSLGLGQDTTVPIHIFTDRQDNIVCVRTGGIADTDLGVIREL
                     LK"
     gene            complement(4737..5432)
                     /locus_tag="PKH54_02085"
     CDS             complement(4737..5432)
                     /locus_tag="PKH54_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98704.1"
                     /translation="MKKLIVHAAVAAVVFLAMSITVSSWLNAAADRAEESLGNVTTTK
                     VGVASAADESYCTPELRVILKRVLTSCGIVKEGGGRGCKPMDAQSVAAMSGADFNGLF
                     KPMADRAAIIQFDIESAELDANATSLIDNTFADRRGASYFLIVSRSSPEGSTEYNRDL
                     SERRAAALYNHLKASYRDPDFDQVAGMLWLGEEFAQLEDEFCGWTRSRPDSPCDNTAL
                     NRSAFVAWIDCRL"
     gene            complement(5436..6653)
                     /locus_tag="PKH54_02090"
     CDS             complement(5436..6653)
                     /locus_tag="PKH54_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOC98705.1"
                     /translation="MTSLRTMITSVLFILFALGGAWFAGVSTYDFFLHLDGQVHSITC
                     SYVPGTAERDELGTSGCYAVMMSPYSSVMRDRTWAGIPIALPGLAVFIYMIFIGLEHL
                     LLGRHRKRADAVYLVAASLLPLVTSIAYYYISVTKVGEVCKMCVGIYVMSIGLFLAAV
                     AQLILARREQEPGPHHIRNTILRYLLWFVEGVLFVVIPVIMYMNLKPEYQERNCGELV
                     KPEDRSSIMLPAVGQGVPAIEVLDPLCPSCEVFRNRLADTEVFGRLSMKTVLFPLDSE
                     CNWMLKEPVHHGACLVSRAILCSTDPEKVIDWALENNVEIRKAAKEKGQDAVKAMVLE
                     KFSDVSQCIDTAETKQKLNLSLRWIMANSLPLLTPQLYVNNRRLCDEDMDLGLEYGLG
                     KLIEAGSTTEGGK"
     gene            6919..7737
                     /locus_tag="PKH54_02095"
     CDS             6919..7737
                     /locus_tag="PKH54_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC98706.1"
                     /translation="MDRRLENVKRVLVFTAHADDCEFFCGGIVAALARMGAEITEVIA
                     TDNGRGSFELDQASLVTRSREIEAAAAAAVIGKKHVEFLGFPDGFLDDTPKNELRRIF
                     MAWIRKVRPDCVISFDPFASGETHPDHIRVAQAAVEAVGFAHLPLFHPEQAAEGLLPH
                     LTPISVFFSKSHDTDDTIIDISDVIDVKIAAILAHESQMRLTMTEQRMFLESTGRHPA
                     LVPLFDVDNPGPAIDTIVRKWALDAAEGLPFMYGEGCRVETVDAAFDGFTLPGT"
     gene            7741..>11542
                     /locus_tag="PKH54_02100"
     CDS             7741..>11542
                     /locus_tag="PKH54_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOC98707.1"
                     /translation="MGRRLSAAAVLAGTMAFPCAAMAIPAQVALAGIAFMLPVVPVVA
                     LLLVTGYLAATRNLKLKGFLGRLPGAVILIIVTVFVYTFFLKMSSTLGGLVAPEAESR
                     LVEFVTDNAVLRTDVRRFDEALKMVRESPPGRWEKAVTIPAQVAAREILAGKLLVVDT
                     RLAREYAASHVEGSIRFPRYELEMVTPDWSWQIAADKYLDTLAEIAREQGRRLAFCCS
                     FGVSVSVRTVMELRQRDVDAVMIQGGCGALEDFGVPFSSDYAVSDLDRTVSPDVLDLW
                     IRSGMVNTVVAGWPIIRSNGMDIPHGPIVVVTSGDSQQEKTLRVLTSAIAQSRGIQVV
                     GQASLNRTARFSGMAGSVLFLGAMIEQGLGPMSYFVVLFALLVAIFVVLRRLLQSGEP
                     VAAMRRSAPILALLGTALLVELILIEGTWMASRAWVVSGQHKWIYPILFVLSVGLAFH
                     RFTWPMGVLPSCELTPRPVDGIVSRRGPYLWANLGWSVLLSAILIQASPIVVVMTLAV
                     LGLLVLFNGARYLMVWGLSAGIRIRFAGHFRKPAVPGSPVDPTTDRVANPVVRDELMS
                     RLLLNLAVTAFPGIWFRPGAAGTDMEIRTFVSGQPGKIEVRMPDGSRQLVGIISLQER
                     DGVSVAERIRPRLRLSVAMAGFLGADLEVVFSGGRRLPSVGMVMPDSIARPAGAVPID
                     VILHRQILAMVADDVGLIESGAARDAELLSRERYVEQEAMPTPITFSALARRAARYGA
                     ADAAGTLFGFRKYFSRTVRFGAAVFEPEMARDPGVCGGKVRVRLAKAWIRILDSIFAD
                     FVLPAVVRFTGDLQRHGDRPAHIISSIRQIITRFAFWQEMTALSHAILISAPESTVAP
                     QSERSARCPLPGELNRGLVGGGAFEFLPGDMLPAADSELSIGLVRLTSPFDRDVWRNL
                     PEREHLIARAEYFRGAFRRVVWYLVQVTGRRLREIGRRNGLEDLVFFAGKNELAQVLE
                     PRAGRIDSGFVAELARRQSEWKRLREIPMPVQLRIRDIEDMVIEPGGGMPLVETVEDS
                     TPLRGTLVAGSLPVSGIARVAGADQDVRFAGAAGRILVVPVPDPATVAEMERGSVIIC
                     ESGGRLSHAAMLARELGITMVSGVRGASRRIADGQAITVDSDGVVQTGMPTVAIGLAS
                     ARDGCGNKAGALARLVAAGFPVPDGFVIPPEIVAGIGAASFNDDWAGAVWATVAHLDM
                     GAGFIVRTSSFIEDSRSHSFAGMFRSVAGVGTREALESAVRECISAATGEGVARLARL
                     VGADS"
BASE COUNT         2161 a         3381 c         3649 g         2351 t
ORIGIN      
        1 acaccccgcg caggcagtca gcttcatgaa tcggtgcgtc gcggaagatg actgcctgcg
       61 cggggtgttc ctggtcggcg acctgccgtc ggcgtggtac gagcaggatt ccgattacgg
      121 cggcgacatc ggcatcatgc acgaggaatt tccgacggaa ctcttcttcc aggatctgga
      181 caaccagtgg cgcgacgatg atggcaacgg gattcttgac ggccattccg acttcgccct
      241 ggagatattc tcggcccgcc tggtcggcgg cgttgaaaga atgaagtctt atttcgcaag
      301 gcttcatgaa taccggcaga acggcagctt cttcaacccc cggaatttct tttcgtttat
      361 cgacgacgac tggaacgggg caaactccta cggcgtgccg gacggatcca cgacgaacca
      421 ggaatggggt ctggaatcag tctatggtga caactacatc cgccgggagt ggtccgacaa
      481 caccgacaag accgattatc tcgaggttat gtccggcggc ggcgccgaat tcgtctatca
      541 gtggattcat tcggatcccc agcagatcta ttttgacgac aacttcagcc cgaacccgga
      601 caacatcctg tccctgcagg aacttgtggc ggcagatgta agcggatcgt tcttcaacct
      661 tttcgactgc tcgatcagcc gctataccga gccgatggga aacatcgcga ccgagtacgt
      721 ttattcacgc tatggactgg cgaccgtcgg aagcaccaag acgggcggca tcttcaatcc
      781 ggacgtcctg cacggggcaa tgctggcggg acacggcacc ggccaggcgc tgcgcatgtg
      841 gttcgacgac acctgggcaa accgggatgc ctatggcctt ccggacccta cattcgacga
      901 ctggtggctt ggcatgatga ttcaggggga tcccctggtc ctgcttaacc cgcaggccgg
      961 ttcgagcctg ccgcgttccg tgtccgcaac caccggccct aaatcggaaa gggtgttcga
     1021 acttcgggaa atcgacgccc tttacaggat catgggtcga agggccgcga ccacggtcgt
     1081 gaacggccat cgtgagtaca tgcggaaccg ctgacgcgtc agtccaggtt gcccttgccg
     1141 gtcaggacgt tgccacggtt catcgaaaag atgatcccga ccaggcacag tcccccgaag
     1201 atgcggaaag tcaggctgat gccttccatg aattcggttc gaaccgtctg gtcgatgcgc
     1261 gcgttgccaa gcatgacgcc gaacatgacc gtggcaatgc caagcgacaa gatctggccg
     1321 acgatacgca tcgtggaaag ggttgccgag ccgattccaa accactttcg cggcaggctg
     1381 cgcatgaccg cgttggtatt cggggatgaa aacagggcga atccggcccc gagcgtcatc
     1441 aggcaagcca cgatcatggg cagcccggtt ccgatcgtca cgaatgaaag cgcaatcaac
     1501 ccgcacgcgg tgacggccat cccgaggctg gcaagtatcg tcggctcgac acggtccgac
     1561 aggcgtccgg caagcgggga aaagacggcc tgaacaatgg gctgggtaag cagtatcgtc
     1621 ccggcggcct gcggggtcag tccacggatg ttctgcaggt aaagacttaa caggaatccg
     1681 accccgaatg ttgccatgta attgattaat gccgcgatgt tgctgaaagt gaacgcccgg
     1741 tttccgtaaa acaggcgcat gttcagaatc ggctgtggca cgcggttctc gatgaatgcc
     1801 agcagcagga acagcgcccc cgcgcagccg atcagcgcaa ggccggccgg cctggttatc
     1861 caggtaagcc cgagcagcag gcagacaagc ccggccccaa gcaccaggga cccggccagg
     1921 tcgaaggact cgccttcggc gtccttccat tcacctttca gcagcagcgc ggaaatgaaa
     1981 aggacgacgg ccccgaccgg gacgcaggcc cagaagacgc tgtgccagcc gaatgcatgg
     2041 gtcagccagc ccccgaacgt cgggccgacg gacagtccca catataccga cgccgtgttg
     2101 atcccgagtg cggcgccgcg gcgggccgcc ggaaagatgc tcgtcgtcat ggccatgccg
     2161 gtcgcgaact gcatcgaggc cccgacaccc tgaagcacgc gggcggcaag aaggaacccg
     2221 aagtcgaagg gaagggcgca aagggccgac gcaaccgtga agatggtgac cccgatgatg
     2281 aatacccggc gacgaccatg tatgtcgcca agccggccca tcggcagcac aagagccgcc
     2341 gttgccagca tgtaggaaag cgcaatccag cccagatcca cggcgtccag cccgaaatgc
     2401 gcgccgatgg tcggaagggc gatgtttgaa gacgagccgg cgaatgacgt caggaacgtc
     2461 gccgtggtcg tggtcaggat tccgtaaagc tcggtttttt ccggtttcct gggatcgttc
     2521 atggtgttgt ggccttgcgg catacggtgg cggccggcgg ctagaggccg atgtccgaga
     2581 gtgtgagtgt gtaaatctgg gttgccgtgc cgggaacgtc gcccatcagg atcaggcgcg
     2641 gttttgaact gttttcgtca tagaccatgc gcccgatgtt ctttccgaag ttcggaagcg
     2701 tcttgtcgcc gaccagcgac caggactccc cgtactggcg gaaggttccg gtcgggccgt
     2761 ccgggatgag attggtccag ttcgtgccgt cccatgacca caggtcgttg tgataggtgt
     2821 tcagcctttc atcccagttg tcctcgattt tggcgccgcc gaagacgtac agatgaccgg
     2881 tcgcgccgtc gatcgcgcaa ctgaagcctt cccggcccag cagaccctcc ggcagatcca
     2941 gcgtgcttgc cgacgggccg tcaaggtcga ttatctgaat cctgtcaagc gggtaactgg
     3001 tttcaccgcc atcctcggtc gtcaggccgc caacgcttat caagacgttt tcgacgggat
     3061 cgaaggccag gcagttcgag aatacgggaa gaagtcctgg aagaacggtt gaccagtcgg
     3121 cggttccaag ctcgaacttc agaagcgtgt cgctgaaacc agaaccggta atccagccat
     3181 aaacgaaaaa cgcgtcaccg atgaacaggt gggaatagct gattccacca ttcggcggct
     3241 ggccgtcgtc gccggcggca aggggcaccg gcgtggcgcg ccaggatgtg tcgatgcgca
     3301 tcaggccggg cgaacgcggg cccatgacga tccattcgcc ggcaccggcg tcccatgcga
     3361 tgaactggca ccagtcctcg tccatgcaga aattggtgcc atcggcccac gggctgcctt
     3421 caaggacctt ccgggtgtgt accccggaag actcgtcaag gtgccatgcg tatgaaatca
     3481 gcgggtcttt ccagtcgtgg gtgaactgtg tcaggtattc atcggttacc ggattgaagc
     3541 cgaacgtcat gccatcaacc ggattcatga ttgacgtgcc gcgaacaact tcgtcctcga
     3601 tgcggatgga catcatcttc cagggatttt cgggcggtgt tgcggcgtcc tcgtcggcat
     3661 cctcgatgcc atcggggggc gtgtcgccct ggctctggtc aaccgatgcg tcggcggcat
     3721 ccggcgtgcc ctcatccggg aggcctgtgt cctggccgcc attgtcgggc gcgatcacat
     3781 cacgttcgac atccgtaatt gagtccgggc cgccggtatc gggaagcgat acatccgtgt
     3841 cgattacgac atcgccttcg ccgatatcat tgtcgccgtt gccggaaccg cagccaacgg
     3901 ccatcaggaa cgtcagaagg acaacaggaa cgctcaggct tttcatgaat ctcacccgcc
     3961 agtgataccg gcaaagcata gcccaatttg attcccgcgg catctgttga ttgccgaaat
     4021 tcctgattga agcgggtggc ggccagggtg aggctatttc aggagttcgc ggatcacgcc
     4081 cagatcggta tcggcgatgc cgccggtgcg gacgcagacg atgttatcct ggcgatccgt
     4141 gaagatgtga atcggaacgg tggtgtcctg gccaaggcca agtgattgcg cgaactcgcc
     4201 aagggatccc gggttcgtca tcctctggag cttgacggta cgaagatcgg gccgcctggc
     4261 caggaacgcc tgcaatgtct tcaggtcttc atcgacggaa agcagttcca ggctgatgtc
     4321 ggtaccggtt ccaagcctgg cttcgcgcca tgacaggatg tgcggaattt cggcaatgca
     4381 cggcttgcac caggtggccc acatgttgat ccatctgggc cggccggtga ccatcgccgg
     4441 accctggtgg aacatctcgg tttccggcag cgcgagcttc ggccccctgc ctggatcgaa
     4501 cttgaccgag caccaaccgg gcggaatgac cgcggttccc atattcgcga cagaattgac
     4561 ccgcggcctg ctcgcgttcg ccgcctcgct ggaactgccg gcttcggcct tcagcacgct
     4621 cttcgcgccg tcgatggtct tttcacagcc agcgccgccg aaagcggcca tcgacagggc
     4681 aatgacgaag gagatttccg gaaggtatcg atgcatttta accccgtgat tccaaactac
     4741 aggcggcagt caatccaggc cacgaaggcc gacctgttca gcgcggtgtt gtcgcagggg
     4801 ctgtcgggcc tgctcctggt ccatccgcag aactcgtcct cgagctgggc gaattcctcg
     4861 ccgagccaga gcattcctgc cacctggtcg aagtcgggat cccggtatga cgccttcagg
     4921 tggttataaa gcgcggccgc gcgcctttcc gaaaggtcgc gattgtattc ggtcgaacct
     4981 tccggcgacg accttgagac gatcaggaag tagctggcgc cacgcctgtc ggcgaaggtg
     5041 ttgtcgataa ggctggtcgc gttcgcatca agttcggcgc tttcgatgtc gaactggatg
     5101 atcgcggcgc gatcggccat cggcttgaaa aggccgttga agtcggcgcc cgacatggca
     5161 gcgacgcttt gtgcgtccat cggcttgcat ccgcggccac cgccttcctt cacgattccg
     5221 catgaggtaa ggacgcgctt caggattacg cgcagttccg gggtgcagta cgactcatcg
     5281 gccgccgaag ccacgccgac ctttgtcgtg gtcacgtttc caagtgattc ctcggcgcgg
     5341 tcggccgccg cgttcagcca ggacgaaacc gtaatcgaca tcgcaaggaa cacgactgcg
     5401 gcaaccgccg cgtgaacgat aagctttttc atggctcact tgcctccttc ggtggtggag
     5461 cccgcttcga taagcttccc gagcccgtat tccaggccca gatccatgtc ttcgtcgcaa
     5521 aggcggcgat tgttgacgta aagctgcggc gtcagcagcg gaagggagtt cgccatgatc
     5581 cagcgcaggc tcaggttcag cttctgcttt gtctcggccg tgtcgatgca ctgcgacacg
     5641 tcgctgaact tctcgagaac cattgccttg acggcgtcct ggcccttttc cttcgctgcc
     5701 ttgcggatct cgacgttgtt ttcaagtgcc cagtcgatga ccttctcggg atccgtggaa
     5761 cagaggatag cgcgcgaaac caggcatgcc ccgtgatgaa caggttcctt cagcatccag
     5821 ttgcactcgc tgtcgagcgg gaacaggacg gtcttcatcg acagcctgcc gaagacttcc
     5881 gtatcggcaa gcctgttgcg gaagacttcg cacgacggac acaggggatc cagcacttcg
     5941 attgcgggca ctccctggcc gaccgcggga agcatgatgg aactgcggtc ttccggcttg
     6001 accagttcgc cgcagttgcg ttcctggtat tcgggcttca gattcatgta catgattacc
     6061 ggaatcacga cgaaaaggac gccttccacg aaccacagaa ggtacctgag tatcgtgttc
     6121 ctgatatggt gcgggccagg ttcctgctcg cgacgggcca ggatgagctg ggcgacggcg
     6181 gccaggaaca ggccgatcga catgacgtat atcccgacgc acatcttgca gacttcgccg
     6241 accttcgtga ccgatatgta gtagtaggca atcgacgtga caagcggcag gagcgaggcc
     6301 gcgaccaggt aaaccgcgtc ggcccgcttc cggtggcgcc cgagcagcag gtgttcaagg
     6361 ccgatgaaga tcatgtagat gaatacggcc agtcccggca gggctatcgg gatgccggcc
     6421 catgtgcggt cgcgcatcac cgacgaatac gggctcatca tgaccgcata acagccgctg
     6481 gtgccgagtt catcgcgttc ggcggttccc ggcacgtacg agcacgtgat cgagtgaacc
     6541 tggccgtcaa ggtgcaggaa gaagtcgtac gtggagacgc ccgcgaacca ggcgccgcca
     6601 agggcgaaaa ggatgaaaag caccgaagtg atcattgtgc gcagacttgt catttccgac
     6661 ctcgttgcac ttccaattta atgcggcctt ccctgaaggc ggccgctccc ggatttcgac
     6721 ccgcgaacgg ccagcaagcc atgtccgcga gggcatccga caacggcgcg attctactgg
     6781 caaacgggcg attcgtacaa gctgacggca gtttgcgtca taaagggtta aggcggcccg
     6841 cgtccgcatg ccgcggctgt tgcgcggcca ttgggcgtgt gctaaacaca tcttcctgta
     6901 cggcttcgag gatttgcaat ggacagacgg ctggaaaacg tgaaacgcgt gctggttttc
     6961 accgcgcacg cggacgactg cgagttcttc tgcggcggta ttgtggccgc gctcgcccga
     7021 atgggggccg aaatcaccga ggtcatcgcg acggacaacg gtcgtggcag cttcgagctc
     7081 gatcaggcct cgctggtgac gagaagcagg gaaatcgagg ccgccgccgc cgccgcggtc
     7141 atcggaaaga aacacgttga attcctgggc tttcccgacg gattcctgga cgatacccca
     7201 aagaacgaac tgcgcaggat tttcatggca tggataagga aggtccgtcc cgactgcgtg
     7261 atttcctttg acccattcgc ctcgggtgaa acccatccgg accacatccg ggtggcgcag
     7321 gccgcggtcg aggccgtcgg gttcgcgcat cttcccctgt tccatcccga gcaggcggcc
     7381 gaggggctgt taccccacct gacaccgatc agcgtgtttt tttcaaagag ccatgacacg
     7441 gacgacacga taatcgacat ttcggacgtc atcgacgtca aaatcgccgc gattctggcg
     7501 cacgaaagcc agatgcggct gacgatgacc gaacagagga tgttcctgga atcgactggg
     7561 cgacatccgg ccctggttcc cctgttcgat gttgacaatc ccggtccggc aatcgatacg
     7621 attgtccgga aatgggcgct ggatgcggcc gaagggcttc cattcatgta cggcgaggga
     7681 tgccgcgttg agacggtgga cgcggcgttc gatggtttca cccttccggg gacttgattg
     7741 atgggaaggc gtttatccgc ggcggcggtg ctggccggca cgatggcatt cccgtgcgcg
     7801 gccatggcca ttccggccca ggtcgcgctg gcgggaatcg ctttcatgct tccggtcgtt
     7861 ccggtcgtgg cgctgctgct ggtgacggga tatcttgcgg ccacccgcaa tctcaagttg
     7921 aaaggtttcc tgggcaggct tcccggcgcg gtcatcctga ttatcgtcac ggtttttgtc
     7981 tatacgttct tccttaaaat gtcatcgacg ctgggcggcc tggtggcgcc ggaggccgaa
     8041 tcaaggctgg ttgagttcgt aaccgacaat gcggttcttc gcacggacgt caggcggttt
     8101 gatgaagcgt tgaaaatggt tcgcgagtcg ccccccggcc ggtgggagaa ggcggtgacc
     8161 attccggcgc aggttgcggc ccgcgagatt cttgccggaa aactgcttgt ggtcgacacc
     8221 aggcttgccc gggagtacgc ggcttcccat gtcgaaggtt caatcaggtt cccccgttac
     8281 gaactggaaa tggtgacgcc ggactggtca tggcagattg cggccgacaa gtatctggac
     8341 acgcttgccg agattgccag ggaacagggg cgcaggctgg cattctgctg ctcgttcggt
     8401 gtctccgtgt cggtgcggac ggtgatggaa ctgcgccagc gcgacgtgga tgccgtgatg
     8461 atccagggtg gctgcggtgc cctcgaggac ttcggtgttc cattttcatc cgattacgcc
     8521 gtttccgacc tggacaggac ggtttccccc gatgtcctgg atttgtggat caggtcgggt
     8581 atggtcaaca cggttgtagc cggatggccg atcatccgga gcaacggcat ggatattccg
     8641 catggtccga ttgtcgtggt tacaagcggg gattcccagc aggagaagac cctgcgggtc
     8701 ctgacgtcgg ccatcgccca gagccgcggc atccaggtcg tggggcaggc gtcactgaac
     8761 cggacggcgc gcttttctgg aatggcggga agcgtcctgt tcctgggtgc catgatcgag
     8821 caaggcctgg gcccgatgtc gtattttgtc gtcctgttcg cacttctggt cgccattttc
     8881 gtggtgcttc gcaggctgct tcagtcgggt gagccggttg ccgcgatgcg caggtcagcc
     8941 ccgatcctgg ccctgctcgg tacggccctt ctggtggaac tgattctgat tgaaggtacc
     9001 tggatggcca gccgcgcctg ggtcgtttcc gggcagcaca agtggattta tccaatcctt
     9061 ttcgtccttt ccgttggtct ggcgtttcat cgctttacct ggccgatggg tgttcttcct
     9121 tcgtgcgaac tgactccccg gccggttgat ggaatcgtga gcagacgcgg cccatatctt
     9181 tgggccaacc tcggatggtc ggtgctgctg tcggccatcc tgatccaggc aagcccgatc
     9241 gtcgtggtca tgacgctggc cgtgctcggc ctgcttgtac tgtttaacgg cgcaagatat
     9301 ctgatggtgt gggggctgtc ggccggaatt cggatccgtt ttgccggtca ttttcggaag
     9361 cccgctgttc cgggcagccc ggttgatccg acgaccgatc gggtcgcgaa tccggtagtg
     9421 agggacgaac tgatgtcccg cctgctgctg aaccttgcgg tcacggcatt tcctggcatc
     9481 tggttcaggc ccggagcggc gggaaccgac atggaaatca ggactttcgt ttccgggcag
     9541 cccggaaaaa tcgaagttcg gatgccggac ggttccaggc aacttgtcgg gatcatttcc
     9601 cttcaggagc gtgacggagt cagtgtggcc gaaaggattc ggccaaggtt gcgcctttcc
     9661 gtcgccatgg ccggcttcct tggcgccgac cttgaagtcg tgttcagcgg tggtcgaagg
     9721 cttccttcag tcggcatggt catgccggat tccatcgccc gtccggcagg ggcggttccg
     9781 attgatgtga tacttcaccg gcagatactg gcgatggttg ccgacgatgt cgggttgatt
     9841 gaatccggtg cggcgcgcga tgccgaactg ttgtcaaggg aacgatacgt cgagcaggaa
     9901 gccatgccga caccgattac cttttcggca ctggccaggc gggccgccag atacggggct
     9961 gccgacgccg ccggaaccct ttttggattc cggaagtatt tttccaggac ggtcaggttt
    10021 ggcgccgcgg tctttgaacc ggaaatggcc cgcgatccgg gcgtatgtgg cggaaaggtc
    10081 agggtgcgtc tggcaaaggc ttggatccgg attcttgatt caattttcgc cgatttcgtg
    10141 cttcccgcgg tcgtccgctt caccggtgat cttcagcggc atggcgacag gccggcccat
    10201 atcatttcgt cgatacgtca gatcatcacc cgtttcgcct tctggcagga aatgaccgcg
    10261 ctgagtcacg ccatactgat ttcggcaccg gaatcgaccg ttgcgccgca aagtgaaagg
    10321 tcggcccgtt gtcctcttcc cggcgaactg aatcgtggtc ttgtaggcgg cggggcgttc
    10381 gagttccttc ccggcgacat gcttccggcc gcggacagcg agctttcgat aggcctggtg
    10441 cgcctgacat cgccttttga tcgggatgtc tggcggaacc tgcccgagcg cgaacacctg
    10501 attgcgcgtg ccgagtattt ccgcggcgcc ttccgcaggg tggtctggta cctggtccag
    10561 gtgaccggac gcaggcttcg tgaaatcgga cggcgcaacg ggctggagga tctggtcttt
    10621 tttgccggaa agaatgaact ggcgcaggtg ctcgagccgc gtgccggacg aatcgattcc
    10681 ggcttcgtgg cggaacttgc ccgccgtcag tccgagtgga aaagactgcg ggagatcccg
    10741 atgccggttc agttgcggat ccgggatatc gaggacatgg ttattgagcc gggcggcggc
    10801 atgcccctgg tcgaaacggt tgaagactcc acgccgctgc ggggcacgct ggttgccgga
    10861 tcccttcccg tttccggtat cgccagggtg gctggcgcgg atcaggacgt tcgtttcgcc
    10921 ggcgccgccg gccgcatcct ggtcgtcccg gttcccgatc ccgcgacggt tgcagaaatg
    10981 gaaaggggat ccgtcatcat ctgcgagtcg gggggaaggc tttcccacgc cgcgatgctt
    11041 gcccgtgaac tgggaataac catggtttcc ggtgttcgcg gcgcgtcccg gcgcatcgcc
    11101 gacggacagg ccatcaccgt cgattcggat ggcgtcgttc aaacgggaat gccgaccgtg
    11161 gcgattgggc ttgcgtccgc ccgtgacggg tgcggcaaca aggccggcgc gcttgcgcgg
    11221 cttgtcgcgg cgggattccc ggttccggac ggattcgtca tcccgccgga gatcgtcgcc
    11281 gggatcggcg ccgcctcttt caacgatgac tgggccggtg ccgtctgggc cacggtcgct
    11341 catctggaca tgggcgccgg tttcatcgtc cgtacttcgt ccttcatcga ggactcgaga
    11401 tcccacagct tcgcgggaat gttcaggagc gtcgccgggg tcggtacgag ggaagccctt
    11461 gaatcggccg tgcgcgaatg catttcggcg gccaccgggg aaggggtagc caggcttgcc
    11521 aggttggtcg gggccgattc cg
//
LOCUS       DAOPPA010000037        11414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6522_length_11414_cov_4.416850, whole genome shotgun sequence.
ACCESSION   DAOPPA010000037 DAOPPA010000000
VERSION     DAOPPA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_11414_cov_4.416850"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..952)
                     /locus_tag="PKH54_02105"
     CDS             complement(92..952)
                     /locus_tag="PKH54_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98708.1"
                     /translation="MEEKKSGNTRLTVLMAAVLAVLVLAVAVPLLFGTSEPPTDEAPP
                     VPEQPVQVQQPSQPAARPAGAAARQVPQPNTWCRSYVFSRCNTAGLPASACMGIARVG
                     AEVPMNSGVAGCRAAVEKLIAAEVERMVRQPEAVDDGQAVEPEAAAPEPGTAPEGGQG
                     GESDGSDSARPRSAGDGQQPYRVPAPNVVMKQRDPAADQAAMERMRQLRDEIAVQRNT
                     YVGTNIGVQSRLDEMRDIAGRLETDEAAAAYNNTLKDLGVSSGPPPGPTAVPVQAAPA
                     TPVQVTAQPL"
     gene            1026..2003
                     /locus_tag="PKH54_02110"
     CDS             1026..2003
                     /locus_tag="PKH54_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC98709.1"
                     /translation="MDRPYVSIVLPVFNEVESLDPLFDEIEQVMTGLGRTFEVICCDD
                     GSTDGSKEKLLARASSDDRYRLIFLVRNYGQTAALDAGFRVAEGEVIIPMDADLQNDP
                     ADIPKLLARLEQGADLVSGWRASRKDAFLTKTLPSMIANRLVVSRVTGVNLHDYGCTL
                     KAYRASFLKQFHLYGEMHRLIPAYVKWAGGVVVEEKVNHRARTFGKSKYGLNKTFRLI
                     LDLLTVRFLLGYSTKPLYFIGRYGLAAIGLGCLALTWTIVKKILWHYPLYTDPFFLVS
                     IVLFLGGFQILFSGLLAELSMRTYFESRDRAPYMVARTVNIETDRFRMV"
     gene            2024..3931
                     /gene="asnB"
                     /locus_tag="PKH54_02115"
     CDS             2024..3931
                     /gene="asnB"
                     /locus_tag="PKH54_02115"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOC98710.1"
                     /translation="MCGICGFTGDRHSSDADSIIARMTESLAHRGPDEDGFFVGDGVR
                     LGHRRLSIIDIAGGRQPLFSEDRGIAVVCNGEIYNHSELRAELLERGHRLSSGSDCEV
                     IAHLWEDLGTAMLSRLDGMFAIALWDSRRHELLLARDRMGKKPLFWSATGGGVVFGSE
                     LRALLAHPDVPRRVCPDSLYRFLTLDYVPTPYSIIEGVFKVESGGWILSGSDGITSGR
                     FHELRPGATFDGNEQDAAEAVWEELVRATGRRLESEVPLGVFLSGGLDSCSVLAAMCR
                     HVDPSEISTFTIAFDDPSFDESEAARSVARHFGTRHHEHRLDGEQAVRLVGETASIAD
                     EPLADYSIIPTALLARFAKEHISVALSGDGGDELFYGYETFKADRLARIATRLLPGFV
                     TDRILPAAASAIPVTDRNMGLDYKVDRMSRGLRYDRFGRHLAWVGGFDPRSVRGGGGV
                     LNKSTSDAMSIGEEDWWPDVARVLAGTEHMDPFKRLAIMYMRLYLQDGVLVKVDRASM
                     NCALEVRSPFLDTAMVKLALSMPASLNLHGGATKAVIRRMIRGRVPERIVSAPKKGFG
                     VPVAAWLRSDLRPMIDRLLSREYLARQDIFNPSAVEAMVRSHMERRHNFRKELFSLLI
                     FQLWHEQWICK"
     gene            4027..5877
                     /locus_tag="PKH54_02120"
     CDS             4027..5877
                     /locus_tag="PKH54_02120"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOC98711.1"
                     /translation="MEDLDLVLVIGELVGGLALFIYGMKLMTGALQSAAGTRMRTILG
                     RITGRRVAGLTAGTAMGFMVHSGAGSVMIVGFINAGLLTLAQSIPLMLGNNVGTSLSM
                     QLFSFQVGKYAFFAIGIGLMVATLFKKDAIRQGGLIVLGLGLLFLGMNLMKESAAPLK
                     DAGFFNSFIEAGAAPGILATLSGFLLGIAGSALMQSSGALIGILFAMGAAGVFSSVAQ
                     PLPIVLGAHIGTCVVGLIGSIGTNIESRRSAMAHLMFNAIGGVLALAMAPLYVKAVQF
                     AGGDMVHQIANVHTSVQVVNALIFLPFTNQFTALIRRMTPSTRLPVPKSNLEDKYLQT
                     PEMAIVAVMQETRRMGTMVRKMLRQAMTGVVRPYSDQLFAAIEKEEESVDLLKEAINE
                     YLFEISKRQLSQRQSLIIQHLMRTVADIERIGDHAESITEIVREKINRGVEFDTLSLS
                     RLIDAFRKVDFLLDKMVESLNPANRVFGGHAQVLNSARIEYKQLSEAAREYYKFRIQN
                     KEADAVHGMIYANLFSTMDRIVGHTKSVAKSERDPMFRVKLEKLDMPSPAMEKSMGSD
                     QNYLFIETGIFNTHELDIKMLSFQPPDGNAEVNAPAITFDANSDPGPASE"
     gene            complement(5954..7120)
                     /locus_tag="PKH54_02125"
     CDS             complement(5954..7120)
                     /locus_tag="PKH54_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOC98712.1"
                     /translation="MKSNFVISCLVVASVASACSKSSPIQEPQAPAGGRIEVAEPLFD
                     NGVTVRGEEIRHVFKLKNVGDSAVTILETKASCSCTAAVVSNPVVEPGATADVEVVFN
                     TRGRSGAQSKSVKVHTNSATTPVVILTVSGEVKPAVEFAESNLRLGRVLRNTRVEREI
                     GIVGLRSEGVTLTGLKLSPENDPRLSASIDAADPSKIQVVFNAPAEIGLFRGRIDATT
                     SSPEVPSISMTIMADVTGEVAAQPAEAIFPARGRMTAAGQTSQTIEILLQSMNGKPFT
                     VRTVKDEAGLVKLVSSSKSEEGETIVLEGPLDDNPVQGMLLVEFASSEPLQIPYYSGK
                     TRPGRGIGGPGRGMGGHPGRIPLPGDVRMGKIKVKPWERDAGRPHHIKAPVKGQ"
     gene            complement(7125..7700)
                     /locus_tag="PKH54_02130"
     CDS             complement(7125..7700)
                     /locus_tag="PKH54_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98713.1"
                     /translation="MGTRLIVLLPIIAVLALGTGCKRKSPCEKLADAVCAAGQSEECA
                     NIRRQAEKADEAAGKVCGQILELQPDRSTPAVGCDLLADMVCAERPLEDCLNIRREAK
                     GADLKKAVVCDKFRQMIVDTSKLNQASKQDAMQSGQKAPAARLAPALNVQQADVQGAS
                     AAPAAAQATAPATAPAAQAEPAPAAPTPSVE"
     gene            7969..9303
                     /locus_tag="PKH54_02135"
     CDS             7969..9303
                     /locus_tag="PKH54_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOC98714.1"
                     /translation="MKSYVRSFATMMVVTFAAAGAMAQMAVEPSDGQGDELLPEEPAR
                     QPAYTEVTQPQEQPAEAPAANADKAWGEPADEDDSDEVAGSSTTAEFLKFLREGPAKI
                     KKGSFEMDFHARIQAWGGWVGEDSLLSQGDRMQEYGFRLRRARFGVEGQLVKKLNYKI
                     ELDLFDQEKSGGPLYEAWINYEPTHYFGLTVGLNKFLYSREEMLSSGGLTHLDRSVAS
                     RAMAPGGQMGVMIYSHPVKDKLSIAFGVYNGLQRRASFFEGYEGVGTSLGNKFERMAY
                     VGRIDASPMGDLGGDVADLKNSRPLVGFGAGAMYSNGKTAEIIGASGYAQLKASGFHL
                     LAEVLWDRSKPQADPSVPTVLASQIDRLTFAGSLGYVIPNIKLGLAVRSEYIDDNMNV
                     KDEGDVLITAATVTYYACEHFLKVLVEYQNRYELHGKSISNDAAIAGVQLMF"
     gene            9362..>11414
                     /locus_tag="PKH54_02140"
     CDS             9362..>11414
                     /locus_tag="PKH54_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98715.1"
                     /translation="MIRNLSGKVLAAVLLSAAVVSTGCNEDGQLRAVQISSGDQLIGG
                     PSAKGKMGDYLLENDRIRVIVAGKGPSYAAGMFGGGVLDIDLQRWRAEDRANSGWDSF
                     MESFPLANLLTPNPDGILKEVGFDNGDGSIQLVSQTGELSIVNDGSDGKAAILRMTGH
                     SDYMFDMLKFLNRELIDALLQNLSFSGMTLTPGLLADFVGNYIPGLNLFALLNRLQIG
                     IDFSTDYILEPGKPWLKMATTIRMSPPYDDIVKKCPPVTGCTLECPNGFAMTELSEPS
                     PDGASTPYLRMCPICECANATADMPTFNETRDFFSVLLGTPADWVRPEWKGGVVAGDF
                     LFFGSESPAFVPGLGYDVDRKIYENMYDGVGTMGSPITVDWIAAKGINVSYAWATVNP
                     FEKHGYDCPKYRLAISRIEREDIDFVTDKLKGSAEFDLASNIGVNDARSRVKTAVIDN
                     KPVYLVTVPVPAEGSRPQAFDDWKKSILDNQGKALAAKFGDKVQIDLIAAHECLPSKL
                     MIPLFSSSATAVLTHFTGDAGLQDDGSGNLVDRSRSFTFTRYLTVGEGDIASALRPVF
                     ELRNTPHGELGGVVTQRESGTPLSKVEVFVLEYIQDKDGNDFESFQEYRKAAQEQLRS
                     SGFVTALESDTGIDMSPDGSFAGPVEVGTYYVFAHSPTLGNSQMEKVEITDGGFARVN
                     LS"
BASE COUNT         2251 a         3480 c         3433 g         2250 t
ORIGIN      
        1 cagtttcctg acccgtgtcg gcgacaatgc cgaggacgtg ttcgacgtac ccgtctatcc
       61 catcattgat gtctggtggc ttctgtgatg ctcacagcgg ctgggcggtg acctggacgg
      121 gcgtggccgg cgcggcctga accgggacgg ctgtcgggcc gggaggcggc ccggacgaaa
      181 cgcccagatc cttcagggtg ttgttgtagg cagcggccgc ctcgtccgtt tcaagccgtc
      241 cggcgatgtc cctcatttcg tccagcctgg actgaacgcc gatattggtt ccgacgtatg
      301 tgttgcgctg aaccgcgatt tcatccctta actggcgcat cctctccatc gcggcctggt
      361 cggccgccgg gtctcgctgc ttcatcacga cgttcggcgc gggaacgcga taaggctgct
      421 gcccgtcccc ggcagatcgc ggcctggccg agtccgaacc atccgattcg ccgccctgcc
      481 cgccttcggg cgccgtgccc ggttcaggcg cggccgcttc aggttcgacc gcctggccat
      541 cgtcaaccgc ctcgggctgc ctgaccatcc tctcaacttc ggccgcaatc aatttttcga
      601 cagcggcgcg acaacctgcg acaccggaat tcatcgggac ttccgccccg accctggcaa
      661 tccccatgca ggccgacgca ggcaggccgg cagtgttgca cctggagaac acgtaggagc
      721 ggcaccatgt gttgggctgt ggcacctgac gcgccgccgc gcctgccggc cgtgccgccg
      781 gctgcgaagg ctgctgaacc tggacaggct gttccggcac cggaggagct tcgtcggttg
      841 gcggctcgga cgttccgaac agcagcggga cggccaccgc aagaacaaga accgccagca
      901 cggcggccat caacacggtc aaccttgtgt ttccagactt tttttcttcc acggcgtccc
      961 ccgccagaca aggctcatgt taacatctgt ccagttccgg cccgcatccc cggtggagag
     1021 acacgatgga caggccatac gtttctatag tactgccagt attcaacgag gtggaaagtc
     1081 ttgacccgct ttttgacgaa atcgagcagg tcatgaccgg gctgggcagg actttcgagg
     1141 tcatctgctg cgacgacgga agcactgacg gctcgaagga aaagttgctg gcgcgggctt
     1201 cttccgatga caggtacagg ctgatcttcc ttgtccggaa ctacggccag acagccgcgc
     1261 ttgacgccgg cttccgggtt gccgagggcg aagtcatcat cccgatggat gccgatcttc
     1321 agaacgatcc ggccgacatc ccgaagctgc ttgcacgcct ggaacaaggt gccgacctgg
     1381 tgtccggctg gcgcgccagc cgcaaggacg ccttccttac gaagacgctt ccttcgatga
     1441 ttgcaaacag gcttgtcgtt tcccgggtta ccggggtcaa tcttcacgac tacggctgca
     1501 ccctgaaggc atacagggct tccttcctga agcagttcca tctttacggc gaaatgcaca
     1561 gactgattcc ggcctatgtg aaatgggccg gcggggtcgt tgtcgaggaa aaggtaaacc
     1621 accgggcgcg gaccttcggc aaatccaaat acggcctcaa caagactttc aggctgatcc
     1681 tggatctgct gaccgtaagg ttcctgctgg gatattccac caagccgctc tatttcatcg
     1741 ggcggtacgg gcttgccgcg atcggccttg ggtgccttgc tctgacctgg acgatcgtga
     1801 agaagatcct gtggcattac ccgctttaca cggatccgtt cttccttgtt tcgatcgtcc
     1861 tgtttcttgg cgggttccag atactgtttt cgggcctgct ggccgaactc agcatgagga
     1921 cgtatttcga atcccgcgac agggcgccct acatggtcgc aagaactgtc aacatcgaaa
     1981 ccgaccggtt ccgaatggtt taggctgcag gacgggacaa atcatgtgcg gaatatgcgg
     2041 gttcacgggc gaccggcatt ccagtgatgc cgactcgata attgcccgga tgaccgaatc
     2101 gcttgcacac cgcggcccgg acgaggacgg gttcttcgtc ggcgacggcg tccggctggg
     2161 acaccgacgg ctttcaatca tcgacattgc cggcggacgg cagccgctgt tttccgaaga
     2221 ccgcggaatc gcagtcgtat gcaacggcga aatctacaac cattcggaac ttcgcgctga
     2281 actgcttgaa agggggcacc ggctctcttc cggatccgac tgcgaggtga ttgcccacct
     2341 ctgggaagac ctgggcaccg cgatgctttc caggctggac ggaatgttcg cgatcgccct
     2401 gtgggattca aggcggcacg agctccttct ggcgcgcgac cgcatgggca agaagccgct
     2461 cttctggtcg gcgaccggcg gcggagtcgt gttcggttcc gaactgcggg cgctgctggc
     2521 ccacccggat gttcctcgac gggtctgccc cgattcgctt tacaggtttc tgaccctgga
     2581 ttacgtgccg acgccgtatt cgattatcga gggtgtcttc aaggtcgaat cggggggctg
     2641 gattctttcg ggttcggacg gaatcacttc gggaagattt cacgaactgc ggccgggcgc
     2701 gactttcgat ggaaatgagc aagacgcggc ggaggcggtc tgggaagaac tggttcgcgc
     2761 caccgggcgg cggcttgagt cggaagtccc cctgggggtt ttcctgtctg gcggccttga
     2821 ctcgtgctca gtgcttgcgg cgatgtgccg gcacgtcgat ccatccgaaa tcagcacctt
     2881 caccattgcc ttcgacgacc cctcgttcga tgaatcggaa gccgcccgct cggtcgccag
     2941 gcacttcgga accaggcacc acgagcacag gcttgacggc gaacaggctg tcaggctggt
     3001 gggcgaaacg gcatcgatcg cggacgagcc tcttgccgac tactcgatca tcccgacggc
     3061 cctgctggca aggttcgcga aggagcacat atcggtcgcc ctttcagggg atggcgggga
     3121 cgaactcttt tacggatatg aaacattcaa ggccgaccgg cttgcacgca tcgcgacaag
     3181 gcttcttcca ggcttcgtca ccgaccgcat cctgccggcc gccgcgtcgg ccatcccggt
     3241 gaccgaccgc aacatgggac tggattacaa ggttgaccgc atgtcgcggg ggcttcgtta
     3301 cgaccgtttc ggaaggcatc tggcctgggt cgggggtttt gatccgcgaa gtgtccgggg
     3361 cggcggcggc gtgctgaata aatccacctc ggacgcgatg tcgatcggcg aggaagactg
     3421 gtggcccgat gtcgcgcggg tgctcgcggg aacagaacac atggatccgt tcaagcgcct
     3481 ggccatcatg tacatgcgcc tgtaccttca agacggagtg ctggtcaagg tggatcgcgc
     3541 ctccatgaac tgcgcgctcg aggtcaggtc acccttcctt gacaccgcaa tggtaaagct
     3601 tgccctttct atgccggcgt cccttaacct gcacggcggc gcgacgaagg cggtgatccg
     3661 gcggatgatc cggggacgag tcccggaacg aatcgtgtcc gcgcccaaaa aggggtttgg
     3721 agtcccagtc gccgcgtggc tgcgaagcga tctcaggccg atgattgacc gtctgctttc
     3781 acgcgaatac cttgccaggc aagatatttt caacccgtcc gcggtcgaag ccatggtgcg
     3841 ttcccacatg gagcgacggc acaatttccg aaaggaactt ttcagccttc tgatcttcca
     3901 gctctggcac gagcagtgga tttgtaaata aaaacgattg gcatatatta gggggattga
     3961 ccggcgcgat ccggtcaggg gttgcttcaa cgctggcgct gctcgatttg cttggatgat
     4021 tcgacaatgg aagacctgga tctggtcctt gtaatcggtg aacttgtcgg gggccttgcc
     4081 ctgttcatat acggcatgaa gctgatgacc ggggcgcttc agtcggccgc cggcacgcgc
     4141 atgcgcacca tccttggccg gatcacgggc cgaagggtcg cggggctgac cgccggcacc
     4201 gccatgggtt tcatggttca ttccggcgcc ggatcggtga tgattgtcgg tttcatcaac
     4261 gccggactgt tgacactcgc ccagtcaatc ccgctgatgc tcggaaacaa cgtcggcacg
     4321 tcgctttcga tgcagctctt ttcttttcag gtcggcaagt atgcattttt cgccatcggc
     4381 atcggactga tggttgcaac acttttcaag aaggacgcca ttcggcaggg cgggcttatt
     4441 gtccttggcc tcgggctgct cttcctcggg atgaacctga tgaaagaatc ggccgcgccg
     4501 cttaaggacg ccggcttttt caattctttc atcgaggcgg gggcggcacc gggaatcctc
     4561 gccacgctgt cgggattcct tcttggaatt gccggatcgg ctctgatgca gtcgagtggg
     4621 gcattgatcg gcatcctgtt cgccatgggg gcggccggcg tgttttcatc ggtggcccag
     4681 cccctgccaa tcgttcttgg cgcccacatc ggaacttgcg tggtcgggtt gattggaagc
     4741 atcggcacca acattgaatc ccgacgttcc gcgatggccc acctgatgtt caacgcgatc
     4801 ggcggcgtcc tggcccttgc tatggcaccc ttgtacgtga aggccgtcca gttcgccggt
     4861 ggcgacatgg ttcatcagat tgccaacgtg cacacttcgg ttcaggtggt gaacgccctg
     4921 attttccttc cgttcacgaa ccagttcacg gcgctgatcc gacgcatgac accgagcacg
     4981 cgcctgccgg tgccgaaatc caacctcgaa gacaagtatc tccagacacc ggaaatggcg
     5041 atcgttgccg tgatgcagga aaccaggcgc atgggaacga tggtgcgcaa gatgctgcgc
     5101 caggcgatga cgggcgtggt tcggccttac tcggatcagc ttttcgcggc aatcgagaag
     5161 gaagaggaat cggtcgatct gctgaaggag gccatcaacg agtacctctt cgagatcagc
     5221 aagcggcagc tttcccagcg ccagtcgctg atcatccagc acctgatgag gacggtcgcc
     5281 gacatcgaaa ggattggcga ccacgcggaa tcgattaccg agatcgtccg cgagaagatc
     5341 aaccgcggcg tcgaattcga cacgctgagc ctttcgcgcc tgatcgacgc attcaggaaa
     5401 gtcgatttcc tgctggacaa gatggtcgaa tcgctgaacc cggccaaccg tgtcttcggc
     5461 ggacatgccc aggtgcttaa ttccgcgcgg attgagtaca agcaactgag cgaagcggcc
     5521 cgcgagtact acaaattcag gattcagaac aaggaagccg atgccgtcca cggaatgatt
     5581 tacgccaacc tgttttcaac gatggatcga atcgtcggcc acacgaagag cgtcgcaaag
     5641 agcgaacggg atccgatgtt ccgcgtgaag cttgaaaagc tggacatgcc cagccccgcg
     5701 atggaaaagt cgatgggcag cgaccagaat tacctgttca tcgaaaccgg catcttcaat
     5761 acgcacgaac tggacatcaa gatgctttcg ttccagccgc ccgacggtaa tgccgaagtc
     5821 aacgccccgg ccataacgtt cgacgcgaac tccgaccctg gcccggccag cgaataggaa
     5881 cttccggcag ccgggcagaa ggacacagtc caatcaggca ggtttgaagg ctttcaggac
     5941 gcggtggtgg cgatcactgt cccttgaccg gggccttgat gtgatgtgga cggccggcat
     6001 cacgttccca cggcttgacc tttatcttgc ccattcgaac gtcgccgggc agcgggatcc
     6061 ttcctggatg accgcccatt ccgcgcccgg ggccgccaat gccgcggccc ggccttgtct
     6121 tgccggagta gtacggaatc tgaagcggct ccgatgaagc gaactcgacg agcaacatgc
     6181 cctggacggg gttgtcgtcc agcgggccct caaggacgat ggtttcgcct tcctcggatt
     6241 tgctggacga aaccagcttc accaggccgg cttcatcctt gacggttctg accgtgaacg
     6301 gcttgccgtt cattgactga agcaggattt caatcgtctg cgaggtttgg cccgcggccg
     6361 tcatccgtcc ccgtgcggga aagatggcct cggccggctg ggccgcaact tccccggtca
     6421 cgtcggccat gatggtcatg ctgatggaag gaacctctgg acttgaagtg gtggcatcaa
     6481 tgcgaccgcg gaaaaggccg atttcggcgg gggcattgaa gaccacctgg atcttcgacg
     6541 gatcggccgc gtcaatcgag gccgaaagcc gtggatcgtt ttccggtgaa agtttcaggc
     6601 cggtcagcgt gacgccttcc gaccgaagcc cgacgatacc gatttcacgc tcgacccggg
     6661 tattgcgaag aacccttcca agtcgaagat tgctttcggc gaactcgaca gccggcttca
     6721 cttcgcccga aacggtcagg atcacgaccg gcgtggtggc cgaattcgtg tgaaccttga
     6781 ccgacttcga ctgggcgccg ctgcgccccc gcgtgttgaa gaccacctcc acatcggccg
     6841 tcgcgcccgg ctcgaccacg ggattcgaaa ccaccgccgc ggtgcacgaa caactggcct
     6901 tcgtttccag gatcgtcacg gcggaatccc cgacgttctt caacttgaag acgtgccgga
     6961 tttcctcgcc acgcacggtg accccattgt caaaaagggg ctcggcgacc tcaatgcgac
     7021 cgcccgccgg ggcctgcggt tcctgaatcg gagaggactt cgaacaggcc gacgccaccg
     7081 acgcgacgac aagacacgag atcacaaaat tgcttttcat ttcatcactc aacagaaggc
     7141 gttggggccg cgggggccgg ttcggcctgc gccgcggggg ccgtggccgg ggctgtggcc
     7201 tgtgcggcgg cgggagccgc ggaggcaccc tgcacatcgg cctgctgaac gttcaaagct
     7261 ggggccagcc tggccgccgg tgccttctgg cccgactgca tggcgtcctg cttcgaagcc
     7321 tggttcaact tgctggtatc gacaatcatc tggcggaact tgtcgcagac aaccgccttc
     7381 ttcaagtccg cgccctttgc ctcccgacga atgttcaggc aatcctcgag cggcctttcc
     7441 gcgcagacca tatccgccag caggtcgcat ccaacggccg gtgtcgaacg atccggctgc
     7501 agttccagaa tctgtccaca taccttgccg gccgcctcgt cggccttttc ggcctggcgc
     7561 ctgatattcg cgcattcttc ggactgcccg gccgcgcata cggcatcggc cagcttttcg
     7621 cacggggact tgcgcttgca gccagtcccc agcgccagaa cagcaattat cggaagcaaa
     7681 acaataagcc tggttcccat atacaaaacc cccagaaacg acgcgtggat cgtcgcccag
     7741 taagtggccg ctgtcaatcg cggcggcgca cctcacggca cgcgcggcgg acgcaccttg
     7801 tccaatcagg cggacggatc ccgcgtgaag gaaaagaatg accgccggac ggatcggccg
     7861 ggcgcgatga ttggaatact gttagatttg cagtgttaca ccttattacg gttgaccttg
     7921 ctcctgttcg aacaaataat cctgctggaa tattctggga ggcccccaat gaaatcttat
     7981 gtgcgctctt ttgcgacaat gatggtcgtg acattcgccg cggccggagc catggctcag
     8041 atggccgttg aaccttccga tggtcagggt gacgaactgc tgcccgagga accggcccgg
     8101 cagccggcat acaccgaggt gacgcagccc caggaacagc cggccgaagc cccggcagcc
     8161 aacgcggaca aggcgtgggg cgaaccggcg gatgaagacg attccgatga agttgccgga
     8221 agttccacga cggccgagtt cctgaagttc ctgcgtgaag gccccgcgaa aatcaagaaa
     8281 ggttccttcg agatggactt ccacgccagg atccaggctt ggggcggatg ggtcggtgaa
     8341 gattcccttc tttcccaggg cgaccgcatg caggaatacg gtttcaggct gcgccgggcg
     8401 cgtttcggcg tcgaaggcca gcttgtcaag aagctgaact acaagattga actcgacctg
     8461 ttcgaccagg aaaagtccgg cggcccgctt tatgaagcct ggatcaacta cgagccgacc
     8521 cactacttcg gccttacggt cggcctgaac aagttccttt actcgcgcga ggaaatgctt
     8581 tcatccggcg gcctgacgca cctggatcgg tcggtggcgt caagggcgat ggccccgggc
     8641 ggccagatgg gcgtcatgat ctattcccat ccggtcaagg acaagctgtc gatcgcattc
     8701 ggcgtctata acggactcca gcgccgcgcc agcttcttcg aaggttacga gggcgtcggg
     8761 acgtcccttg gaaacaagtt cgagcgcatg gcctacgtcg gacgcatcga tgcaagcccg
     8821 atgggcgacc ttggcggcga cgtggccgac ctgaagaaca gccgtccact ggtcgggttc
     8881 ggcgccggcg ccatgtacag caacggcaag acggccgaaa tcatcggcgc gtcgggttac
     8941 gcgcagttga aggcctcggg cttccacctg ctggccgaag tcctgtggga tcgcagcaag
     9001 ccgcaggcgg atccttcggt gccgaccgtg ctggcttccc agatcgatcg cctgaccttc
     9061 gccggatccc ttggttatgt cattccgaac attaaactcg gcctggcagt cagatccgaa
     9121 tacatcgacg acaacatgaa cgtgaaggac gagggcgacg tgctgatcac tgccgccacc
     9181 gtcacatatt acgcgtgcga gcacttcctg aaagtcctgg tcgaatacca gaacaggtac
     9241 gagctccacg gaaaaagcat cagcaacgac gccgccatcg ccggcgtcca gttgatgttc
     9301 tgattgcgcc caagcaccac ccggcggggc cttcggacca cccgggtaag tgaggtgtcc
     9361 catgatacga aacctttcgg gaaaggtcct ggcggccgtc ctcctgtctg cggcggtcgt
     9421 ttcgaccggc tgcaacgagg acggtcaact cagggccgtt cagatatcaa gcggcgatca
     9481 gttgatcggc ggcccttcgg caaagggcaa gatgggcgac tatctgctgg aaaatgatcg
     9541 catccgcgta attgtcgcgg gcaaagggcc atcatacgcg gccggcatgt tcggcggcgg
     9601 cgtcctggac atcgatctgc agcgctggcg cgcagaggat cgcgcaaaca gcggatggga
     9661 ttcgttcatg gaatccttcc cgcttgcgaa cctgctgacc ccgaatccag acggaatcct
     9721 gaaggaagtc gggttcgaca acggcgacgg ttcaatccag ctcgtcagcc agacgggcga
     9781 actcagcatc gtcaacgacg gctcggacgg caaggcggca atcctccgca tgaccggaca
     9841 ctccgactac atgttcgaca tgctgaaatt ccttaaccgc gaactgatcg acgcgcttct
     9901 tcagaacctc agcttcagcg gtatgacgct gaccccgggg ctgctggccg atttcgtcgg
     9961 caactacatc ccgggcttga acctgttcgc cctgctgaac cgccttcaga tcgggatcga
    10021 cttctcgacc gactacatcc tggaacccgg caagccatgg ctgaagatgg cgacaacaat
    10081 caggatgtcc ccgccgtacg acgacatcgt gaagaagtgc ccgcccgtca cgggctgcac
    10141 gcttgaatgc ccgaacggct ttgcgatgac cgaactgtcc gagccaagtc ccgacggcgc
    10201 aagcacgcct tacctgcgca tgtgcccgat ctgcgaatgt gcgaacgcga cggccgacat
    10261 gccgaccttc aacgagacca gggacttctt ctcggttctt cttggaacgc ctgccgactg
    10321 ggtaaggccg gaatggaagg gcggggtcgt cgccggcgac ttcctgttct tcggttcgga
    10381 aagtcccgcg ttcgtccccg gcctgggtta cgacgttgac cgcaagatct atgaaaacat
    10441 gtacgacggc gtcggcacga tgggaagccc gataacggtt gactggattg ccgcaaaggg
    10501 catcaacgtc agctatgcat gggcaacggt taacccgttc gaaaagcatg gctacgactg
    10561 tccgaaatac cgccttgcga tctcgcggat cgaacgcgaa gacatcgact tcgtgaccga
    10621 caaacttaag ggaagcgccg aattcgacct ggccagcaac atcggcgtaa acgacgccag
    10681 atccagggtc aagaccgcgg tcatcgacaa caagcccgtt taccttgtga cggttccggt
    10741 gccggccgag gggtcacgcc cccaggcctt cgacgactgg aaaaagtcga ttcttgacaa
    10801 ccagggcaag gccctcgccg cgaaattcgg cgacaaggtt caaatcgacc tgatcgcggc
    10861 acacgagtgc ctgccgtcca agctgatgat tccgttgttc tcgtcctcgg ccaccgcggt
    10921 tctgacccat ttcacgggcg acgcgggact tcaggacgac ggctccggaa atcttgtcga
    10981 tcgcagccgc agcttcacct tcaccaggta cctgactgtc ggcgagggcg acatcgcatc
    11041 cgcgctgcgt ccggtgttcg aactgcgaaa caccccgcac ggcgaacttg gcggcgtcgt
    11101 tacccagagg gaatccggaa cgccactgtc gaaggtcgaa gttttcgtac tcgagtacat
    11161 ccaggacaag gacggcaacg atttcgagtc gttccaggaa taccgcaaag ccgcccagga
    11221 acagcttcgt tcgtccggct tcgtgaccgc acttgaatcg gacaccggca tcgacatgag
    11281 cccggacggt tctttcgccg gcccggtcga agtcggcacg tactatgtat tcgcgcacag
    11341 cccgaccctt ggcaacagcc agatggaaaa ggtcgaaatc accgacggcg gtttcgcaag
    11401 ggtcaacctt tcgc
//
LOCUS       DAOPPA010000038        11147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6711_length_11147_cov_3.885683, whole genome shotgun sequence.
ACCESSION   DAOPPA010000038 DAOPPA010000000
VERSION     DAOPPA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_11147_cov_3.885683"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKH54_02145"
     CDS             <1..889
                     /locus_tag="PKH54_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC98716.1"
                     /translation="AAAGKDNFLMFGEAFDGNDNLIGSFTAPGMLDSVFYFSQKFVID
                     DIVKNGAPTRRIEDLWKSRIPTPESDPRTLNYGTTPQPGGVTAADGTGLPPWRLTVNF
                     LDNHDLPRFMYEWDDPAALKVALALLLTMDGIPCIYYGTEQDFAGGNDPANREDLWDT
                     GYATDGATFAWIRQLNYLRRDHEPLRRGDMKVVWSSTNTGNAQDAGILAYERTVPATG
                     GSPARTLLVVLNLSATNTATTSADESGGGDMVTSFAQGTKLVNLLDEPDPTDDVTVGQ
                     GGTVRLTLGPRTAKVFAVR"
     gene            914..2002
                     /gene="ugpC"
                     /locus_tag="PKH54_02150"
     CDS             914..2002
                     /gene="ugpC"
                     /locus_tag="PKH54_02150"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOC98717.1"
                     /translation="MAELKLRGVGKVYDGGVRVLSGIDLDVADGEFLVLVGPSGCGKS
                     TLLRMIAGLEHITEGTLEIDGRVVNDVAPKDRDVAMVFQSYALYPHMTVRQNMGFSLQ
                     IRRTAAAEMDAKVEEAARMLGLSGLLDRLPRELSGGQRQRVAIGRAIVRRPRVFLFDE
                     PLSNLDANLRGQMRVELKALHRDLGTTMIYVTHDQIEAMTLADRICLLEGGHARQVDT
                     PSGLYDRPDSRFVASFIGSPPMNFLPLAPAGNRLTAAGISIPMPAGLPSDRLRATVDV
                     GLRPHELGLAETDGGSIDATVDLIEMLGWDALVHARCGDVPVLAQIEAHGAAGLEHGA
                     PIRFWAAPGAAKLFDHETGRAIKGGGAA"
     gene            1999..4185
                     /locus_tag="PKH54_02155"
     CDS             1999..4185
                     /locus_tag="PKH54_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOC98718.1"
                     /translation="MRRWMLMALVFAALLAVPPGVRGDAPAPATLRLWHSYRGAERDA
                     VERVVAEFARIHPEWPVELLAVPYEAFANKLTSAIPRGNGPDVFIAAHERLGDWVESG
                     LIAGLPEGDPWSGFFPATVEAVTWKGRQFGYPLAFKSLALFVRDDVLGGPVPGDTDSL
                     QAACRRMAAGRPGNFCIAWEAGSFYHHAPWVFGFGGGILDKDGKPRLDRPENAASVAF
                     VTGLTKDGHMPQEPTSALITQLFNEGRVAMAVNGPWFLGELAPGVKYSVHPLPRVSST
                     GLPATPFLTVDAAMVSSKSTMPEAAGALARFLAVEGAAPRLVDARQVVALASAWDLTD
                     VAVDPIVVQFREAAAAAIPMPNVPVMRAVWEPASQALRAALRGAAEPEAAMESAMQRI
                     EIATRPRPAAANPLFALAGLAILGIAALVWAARRFRRDDTWSRMRASSHAYAFLAPAA
                     VATGVLVFVPFAVGTGVAFFSHHQGEFTYVGLANFINILGSTDYAVTEPLSFWFTLGV
                     TVMWTVVNVFMHVSIGVALALVLRRPWMKLRGVYRVLLIVPWAVPSYITALIWKGMFH
                     KQFGAVNGLLVWLGLEPVSWFSHFWTSFAANVATNTWLGFPFMMVVTLGALQSIPGEL
                     EEAAEMDGATAVQRFFSITLPLLKPALLPAIVLGGVWTFNMFNIIFLVSGGEPGGSTE
                     ILISEAYRWAFSRQEQYGYAAAYATLIFLFLLAWSLAAGRLQRRTA"
     gene            4182..5033
                     /locus_tag="PKH54_02160"
     CDS             4182..5033
                     /locus_tag="PKH54_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOC98719.1"
                     /translation="MNRRPSWLAAVGWNLALVVACAVTLYPVLWVIKMALTPSQAFDL
                     SPSPIPNTFSLSNFHDLVMATDMAGRWLFGHWLFNSVVVAGATTLLSLALATTAAYAF
                     SRFVFPGRRIGMTLFLVTQMFPGVVMAIPLYLLLDAMNLLDSMVGLTLVYSTTAIPFC
                     VWMMKGYFDTIPKDLEEAAVMDGAGRWTMFFRIILPLARPAIVVTALFSFMTAWNEFI
                     LAATFLSDETAFTLPVALQRYVGDYSTEWGHFAAGAIIVSTPVMALFFALQRHLIGGL
                     TAGGVKG"
     gene            5306..5551
                     /locus_tag="PKH54_02165"
     CDS             5306..5551
                     /locus_tag="PKH54_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC98720.1"
                     /translation="MTSKTNTQITDIGRFLRARRKERGITQAELARHAEVSYTLVNRI
                     ENGDMNVQAVSLNKVLGVFGYRIGPVPRDRSVEESDV"
     gene            5544..6908
                     /locus_tag="PKH54_02170"
     CDS             5544..6908
                     /locus_tag="PKH54_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOC98721.1"
                     /translation="MSDVEYSQITKAYVYLRDARVGVLERQARDRFAFQYDQDWLSMP
                     GSPDISCAMKKRPEPFVERTLHPFFDNLIPEGWLLQNAESLLHIDKANRFAILMATGR
                     LPTGAVSVRAIDSQGREVDALSGILDRGLSDGLVEIPLSGPVGFCPTCFKPLAQGAIH
                     RKCVTAMWGTTRKLKVAVYPDRPLVSFAHVLYGGSISGAQKKGAFHLDTSRGLLRATP
                     VDAQYVLKPDGDFPELPENEHATMAIAMAAGFDVPPFALVNIPGLGNVFAIRRFDITE
                     SGTRLMMEDMGQLIRVPADDKYDSTFERVAAAIRAYSSAPEIDIGDFFRRLVFCFLIA
                     NGDMHLKNWSLLENERALGTFKLAPCYDMLNTRLPIPRERSETGLKMQGRDRNLKASY
                     FKAFGRSIGLSESRIGLVFGELERWLEIVRTMVGQSLLQPRSKERYVEIVQERYRILT
                     SSGC"
     gene            6991..7764
                     /locus_tag="PKH54_02175"
     CDS             6991..7764
                     /locus_tag="PKH54_02175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC98722.1"
                     /translation="MSDIFQFDEHFWQYFHELFEALPRQGPGLDESTRHALGLLPPLG
                     PDQSILDIGCGSGVQTLELARRSPARITATDLHRPFLEILKRNAMAQGLDERISTVVA
                     DMADLPFPDRSFDVVWAEGCSFIVGFARALELWRRLLKPGGRMVVSEFTWFSDDIPAE
                     LREFCVPDPTEDASPAGRRASIAAAGYELLHEFRLPPEGWSKSYYGPLQVQLDGFAAR
                     HAGTPAAMNVVAHCRQELELSRKYGDRYGYTFFIMGLRD"
     gene            complement(7929..8972)
                     /locus_tag="PKH54_02180"
     CDS             complement(7929..8972)
                     /locus_tag="PKH54_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOC98723.1"
                     /translation="MDIIATEKSDTPQRDAWFDNVKFVLIFLVVAGHMLEPLDYGPAY
                     LLIYSFHIPLFVFVSGYFSRNFAKAGNERKLIERLVIPYIVFEVAYSVFDWWLFDRPK
                     LTFSPFSPYWITWFLFSMVLWRASLPYVSRLRMALPISLLVGLAAGYSGSIGYFGSLS
                     RTLVFMPFFLAGVVFKPRVLQDALRPPVRGAVFRILAVILLASAYAFFLTGDRDRLHQ
                     WFYGSFSYESMRIGGWLAAGYRLCEYAVACVLSASVLILVPSRKIPLLTASGRNTMYV
                     FLLHGFVARLWLESKWFQQFDSISGKAMILGGALIITVILSSRAVRIVFHPLVEPRLD
                     FLFKRGQDGPTKC"
     gene            complement(9005..10153)
                     /locus_tag="PKH54_02185"
     CDS             complement(9005..10153)
                     /locus_tag="PKH54_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98724.1"
                     /translation="MKSTAFILFLLTASLLVACGGGTTDADSGQDTGIQDVATDLGTQ
                     DPGTQDPGGADHGASDAVIQDSATDSGSDVDEDVPTDATEPENIGLNPMYSECGGFQP
                     ETRLAEEDPNCGDETLEWDVDSESGVLTFTNTDVFLNCCGDHDIQVTVQDGVYVITET
                     DRPAAGEGRCFCQCLFDFQIQIPPIDGDAIDVRIERVVTDEGTGEAVVAWEGRIDTTT
                     GQGIVTIKEDVGWCNQGIDIGTNPRASGCGGFDAPRSATSGETGCPDEVLNWQFDAVG
                     QTLAFINSSVDLNCCGIHEIQAFFDAGVYHLAETDAPDDESGRCKCMCLFDFAIDVSD
                     VVGPTIEVRLSRDLTDDDFDRGVVWEGSIDLLAGSGTITIRENTGACD"
     gene            10416..>11147
                     /locus_tag="PKH54_02190"
     CDS             10416..>11147
                     /locus_tag="PKH54_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98725.1"
                     /translation="MKVGVLTNLNSRRNRAGGSDLPAKGLKGSCVVRRTSDISEIRPA
                     LEEFIDMGCDCWVADGGDGTLHWMLNEGREVLKKRGMWNGGSAYPMIVPSNSGTIDFV
                     ARTVGIRGTTDQVVRSLLDGGGQDDDFETVCLDTIDVRGHVHGDPDGTWAFERTGFAI
                     AIGGIGQKFFAKYYQRPHRNPLSIIDISARTSVGYIASLLPEKLESVFPDDVLETGRY
                     ILSGTRADVVADGRKFDYGVYQGLHV"
BASE COUNT         2093 a         3272 c         3505 g         2277 t
ORIGIN      
        1 ggcggcggcc ggcaaggaca atttcctgat gtttggagag gcctttgacg gcaacgacaa
       61 tctgatcggc agtttcacgg cgccgggcat gctggattcg gtgttctatt tttcgcagaa
      121 gttcgtaatc gacgacatcg tcaagaatgg tgctccgaca aggcgcattg aagacttgtg
      181 gaagagcagg attccgacgc cggaaagcga cccgcggacg ttgaactacg gcacgacgcc
      241 ccagccgggc ggcgtcacgg cggcggatgg caccggactg ccgccgtgga ggctgacggt
      301 caatttcctg gacaaccacg acctgccgcg gttcatgtac gaatgggatg atccggcggc
      361 cctgaaggtc gccctggcgc tgctgctgac gatggacggc attccatgca tctactacgg
      421 gaccgagcag gatttcgccg gcggcaacga cccggcgaac cgcgaggatc tgtgggacac
      481 cggatacgcg accgacggcg cgacgtttgc ctggatcagg caactgaatt atctgcgccg
      541 cgaccacgaa ccgcttcggc gcggggatat gaaggtcgtg tggtcctcga cgaacaccgg
      601 aaatgcccag gacgccggaa ttcttgcgta cgaaaggacg gttcccgcaa cgggcggctc
      661 gcccgccagg actctgcttg tggtcctgaa cctgtccgcg accaacacgg ccacgaccag
      721 cgcggacgag tcgggcggcg gcgacatggt cacgtcgttt gcccagggga cgaaacttgt
      781 gaacctgctt gacgagccgg atccgaccga cgacgtcacg gtcgggcagg gcggaacggt
      841 gcgcctgaca ctggggccgc ggacggcgaa ggtcttcgcg gtgcgctgaa tctggcgcga
      901 cgggggttgc tgcatggcgg aattgaagct gcgcggggtt gggaaggttt atgacggcgg
      961 ggtgcgcgtc ctgtcgggca tcgatctgga cgtggcggac ggcgagttcc tggtgctggt
     1021 cgggccgtcg ggctgcggga aatcgacgct tctgcggatg atcgcgggac tggagcacat
     1081 aaccgaaggc acgcttgaga tcgacgggcg cgtggtcaac gacgtggcgc cgaaggatcg
     1141 cgacgtcgcg atggtgttcc agtcgtacgc cctgtacccg cacatgacgg tgcggcagaa
     1201 catgggcttt tcgttgcaga ttcggcggac cgcggcggcc gaaatggacg ccaaggtcga
     1261 agaggccgcc aggatgctgg gcctttcggg gctgctggat cgactgccac gcgagctttc
     1321 gggcggccag cgccagcgcg tggccatcgg gcgcgccatc gtccgcaggc cgcgggtgtt
     1381 tcttttcgac gagcccctgt cgaacctgga cgccaacctg cgcggccaga tgcgggtcga
     1441 actgaaggcg cttcaccgcg accttggaac cacgatgatc tacgtcacgc acgaccagat
     1501 tgaagcgatg acgctggccg acagaatctg cctgctggaa ggcggccatg cccgtcaggt
     1561 cgatacaccg tccggccttt acgaccgtcc cgattccaga ttcgtcgcat ccttcatcgg
     1621 ttcgccgcca atgaatttcc tgccgcttgc gcctgcggga aaccggctga cggcggcggg
     1681 gatttcgata ccgatgccgg ccggtctgcc gtcggatcgg ctccgggcga cggtcgatgt
     1741 cggtctgcgg ccccatgaac tggggcttgc cgagaccgat ggcgggtcga tagatgcaac
     1801 cgtcgatctg atcgagatgc ttggctggga tgccctggta cacgcccgtt gcggcgatgt
     1861 ccccgttctg gcgcagattg aggcgcatgg ggcagccggg ctggaacacg gtgcgcccat
     1921 caggttctgg gcggcccccg gggcggccaa gttgtttgac catgaaaccg gtcgcgcaat
     1981 taagggtggg ggtgcggcgt gagacgatgg atgctgatgg cgctggtgtt tgccgcgctg
     2041 ctggccgtgc cgcccggggt caggggtgac gcccccgccc cggccaccct gcgtctttgg
     2101 cattcgtacc gcggggccga gcgcgacgcg gtggagcggg tggtggccga gttcgcccgg
     2161 attcatcccg aatggccggt cgagctcctg gccgttccgt acgaggcgtt cgccaacaag
     2221 ctgacgtccg cgattccgcg tggcaacggc ccggacgttt tcatcgcggc ccacgaacgc
     2281 cttggcgact gggtcgaaag cggcctgatc gccgggttgc ctgaaggcga cccatggtcc
     2341 gggttctttc ccgccacggt ggaggcggtg acctggaagg ggcgccagtt cggatacccg
     2401 ctggcgttca agtcgctggc gcttttcgtg cgcgacgacg tgcttggcgg tccggttccg
     2461 ggcgacaccg actcattgca ggctgcctgc cgcaggatgg ccgcgggcag gccgggcaac
     2521 ttctgcatcg cctgggaggc cggcagcttc tatcaccatg cgccatgggt gttcggtttc
     2581 ggcggcggca tcctggacaa ggatggcaaa cccaggctgg acaggccgga aaacgccgcg
     2641 tcggtggcgt tcgtcaccgg cctgacgaaa gacggtcaca tgccccagga gcccacatcg
     2701 gcgctgatca cccagctttt caacgaaggc cgcgtcgcga tggcggtcaa cggtccttgg
     2761 ttcctgggcg aactggcccc cggggttaag tactcggttc atccccttcc ccgcgtctcg
     2821 tcaaccggtc ttccggcgac gccattcctg accgtcgatg ccgccatggt gtcttcgaaa
     2881 tcgaccatgc cggaagccgc tggcgcgctg gcccgctttc tcgccgtcga gggtgcggcg
     2941 ccccgtctcg ttgacgcccg ccaggtcgtg gcccttgcgt ccgcctggga tttgaccgac
     3001 gtcgccgtcg atccgatcgt cgttcagttc cgcgaggccg cggccgcggc aatcccgatg
     3061 cccaacgtcc cggtcatgcg ggcggtgtgg gagccggcct cccaggccct gcgtgcagcc
     3121 ctgcgcggcg ccgccgagcc ggaagccgcc atggaatcgg ccatgcagcg aatcgaaatt
     3181 gccacgcgcc caaggccggc cgctgcaaac ccgttgttcg cgctggcggg cctggccatc
     3241 ctcggcatcg ccgcgctggt atgggcggcc cgccgcttca ggcgcgacga tacgtggtca
     3301 cggatgcgag cgtcgtccca cgcctatgcc ttcctggccc cggcagccgt ggcgaccggc
     3361 gtgctggtgt tcgtcccgtt cgcggtcggc accggcgtgg cgttcttttc gcaccaccag
     3421 ggcgaattca cttacgtcgg ccttgccaac ttcatcaaca tcctgggaag caccgattac
     3481 gccgtgacgg agccgctttc gttctggttc acccttggcg tgaccgtcat gtggaccgtc
     3541 gtcaacgtct tcatgcacgt atcgatcggc gtggccctgg cgctggtgct gcgacgtccg
     3601 tggatgaagt tgcgcggcgt ctatcgcgtg cttctgattg ttccgtgggc ggtgccgagc
     3661 tacatcaccg cgcttatctg gaagggtatg ttccacaagc agttcggcgc ggtaaacggc
     3721 ctgctggtct ggctgggact tgaaccggtg tcttggtttt cccatttctg gacgtcgttc
     3781 gcggccaacg tcgcgaccaa cacctggctt ggattcccgt tcatgatggt ggtcacgctg
     3841 ggcgcgctgc agtcaatccc gggcgagctc gaggaggccg cggaaatgga cggcgccacg
     3901 gcggtccaga ggttcttctc gataacgctg ccgctgctga aacccgcgct tctgccggca
     3961 atcgtgcttg gcggcgtctg gaccttcaat atgttcaaca taatcttcct cgtcagcggc
     4021 ggcgagccgg gcggctctac cgagatcctg atatccgaag cgtaccgctg ggcgttctcg
     4081 cgccaggagc agtacgggta cgccgcggca tacgcaacgt tgattttcct gttcctgctg
     4141 gcctggagcc tggccgcggg ccgcctgcaa aggaggacag catgaaccgt cgtccgtcct
     4201 ggcttgccgc agtcggctgg aacctggcgc ttgtcgtcgc gtgcgcggtc acgctttatc
     4261 cggtcctgtg ggtcatcaag atggccctga ccccgtccca ggcgttcgac ctgtcgccaa
     4321 gcccgattcc caacacgttc agcctgtcga acttccatga ccttgtcatg gccaccgaca
     4381 tggccggccg ctggcttttc ggccactggc tgttcaactc ggtcgtcgtc gccggcgcga
     4441 ccacgctgct cagcctggcg cttgcaacga ccgcggccta tgcgttttca cgtttcgtct
     4501 tccctggccg ccgaatcggg atgaccctgt ttctggtgac gcagatgttc ccgggcgtcg
     4561 tcatggcgat tccgctttac ctgctgctgg acgcgatgaa cctgctcgac agcatggtcg
     4621 gcctgacgct tgtgtattcg accaccgcga ttccgttctg cgtgtggatg atgaagggtt
     4681 acttcgacac gatcccgaag gatctggaag aggccgcggt catggacggc gccggccgct
     4741 ggacgatgtt tttccggatc atcctgccgc ttgcgcgccc cgcgattgtc gtgaccgcgc
     4801 tgttttcctt catgacggca tggaacgagt tcatcctggc ggcgacattc ctgtccgacg
     4861 agacggcctt cacgttgccg gtcgcgctgc agcgatacgt cggcgactac tcgaccgagt
     4921 ggggacactt cgccgcgggc gccatcatcg tcagcacgcc ggtcatggcc ctgttcttcg
     4981 cgctgcagcg tcacctgatc ggcggactga ccgccggcgg cgtcaagggc tagaatttcg
     5041 ctggtgcgga ttgaacgttg cgtacctggg gattccggcc ctgcgggagt cagtccattg
     5101 gatattcccg gtcgggaata attggtacaa aatgttctta aatcaggttg ttttctcaat
     5161 attgtccttt tttaaggata attcttgcag attgtcttgt tcagatcctt ttttgaggac
     5221 aaaatacggg atatcaccag tcgacatcct tattttggga tgaaatatcg acagattgtt
     5281 gtcgggcgtc aatggggatg acggtatgac cagcaagacc aatacgcaga ttacggatat
     5341 tggaaggttc cttcgtgcca gaaggaagga gcgtggcatt acccaggctg aacttgccag
     5401 gcatgctgag gtcagttaca cactggtcaa tcgaatcgaa aacggcgata tgaatgtgca
     5461 ggcagtttcc ctgaacaagg ttcttggggt ttttggttac cggatagggc cggttccacg
     5521 cgacaggtca gtcgaggaga gcgatgtctg acgtcgaata ttcgcaaatc acaaaggcct
     5581 acgtgtatct gcgtgatgcc agggtcggag tgctggagcg tcaggccagg gacaggttcg
     5641 cttttcagta tgaccaggac tggctgtcga tgccgggttc gcccgacatt tcatgcgcca
     5701 tgaagaaaag gccggagccg ttcgtcgaaa ggacgcttca tccatttttc gacaatctga
     5761 ttcccgaggg ctggctgctg cagaatgctg aatcgttgct gcatatcgat aaggcaaaca
     5821 ggtttgccat attgatggcc acgggccgtc tgcccaccgg ggcggtttcg gtcagggcga
     5881 ttgatagtca gggacgggaa gttgatgctt tgtcagggat cctggacaga ggtctatctg
     5941 atggccttgt cgagatcccg ctgtcggggc cggtgggatt ttgtccgacg tgtttcaagc
     6001 cgcttgcaca gggtgcgatt catcgcaagt gtgtcacggc gatgtgggga accaccagga
     6061 agctgaaggt cgcggtgtat cccgacaggc cgctggtctc gttcgcgcac gtcctttatg
     6121 gcggatcgat atccggggca cagaagaagg gtgcctttca cctggatacc agccgcgggt
     6181 tgctgcgggc gacgccggtt gacgcgcagt atgtactgaa acccgacggc gactttccgg
     6241 aactgccgga aaatgaacat gcgacgatgg cgattgccat ggccgccggg tttgatgtgc
     6301 ctccgtttgc attggtcaac attccagggc ttggaaacgt gttcgcaatc agacgattcg
     6361 acatcaccga atcaggcacg cgtcttatga tggaagacat gggacagttg attcgagttc
     6421 cggctgacga caagtacgac tcgaccttcg aaagggtagc tgcggcaatc cgggcgtatt
     6481 catcggcccc cgaaatcgac atcggggatt ttttcaggcg ccttgttttc tgcttcctga
     6541 ttgcaaacgg tgacatgcac cttaagaact ggtcgctgct ggaaaacgaa agggcgctgg
     6601 gaacgttcaa gctggcgccg tgttatgaca tgctgaacac ccgattgcca atacctcgag
     6661 agcggtctga aacgggcctg aagatgcaag ggcgcgatcg gaatctgaaa gcgtcgtatt
     6721 ttaaggcgtt cggcaggtcg atagggttgt cggaatccag gattggcttg gtgtttgggg
     6781 aacttgagag gtggctcgag attgtccgga ccatggttgg tcagtccctg ctgcagccac
     6841 gctcaaagga acgatatgtg gaaattgtcc aggagcgata tcgaattctg acttcatccg
     6901 gttgttgaat gattcgaccg cgaagtttcg cgccacaaga ggtttcggaa ccctggggct
     6961 ggttggattc acgaacacaa ggaggttcca atgtccgaca tatttcagtt cgacgaacat
     7021 ttctggcagt actttcacga gctttttgag gcacttccac gtcagggacc cggcctggac
     7081 gaatccacca ggcacgcgct gggcctgttg ccgccgcttg gccccgacca gtccattctt
     7141 gacatcggat gcggatccgg cgtgcagacg cttgaactgg cgcggcgcag tccggcccga
     7201 attaccgcga ccgacctgca ccgcccgttc ctggaaatcc tgaagcggaa cgcgatggcc
     7261 caggggcttg atgaacggat ctcgaccgtg gtcgccgaca tggccgacct tccatttccg
     7321 gatcggtcgt tcgacgtcgt gtgggcggaa ggctgcagct tcatagtcgg gttcgcgcgc
     7381 gcacttgaac tgtggcgccg gctgttaaag cctggcggcc gcatggtcgt ttccgagttc
     7441 acgtggtttt ccgacgacat ccccgccgaa ctgcgtgagt tctgtgtgcc ggacccgacc
     7501 gaggacgcca gcccggccgg ccggcgggcc tcgattgcgg ctgccggata cgaactgctg
     7561 cacgaattca ggctgccgcc cgagggctgg tcgaagtcgt actacgggcc gcttcaggtt
     7621 cagcttgacg ggttcgccgc caggcatgcg ggcacgccgg ccgccatgaa cgttgtcgcc
     7681 cattgcaggc aggaactcga gctttcaagg aagtatggcg accgctacgg ttatacgttc
     7741 ttcataatgg gattgcgtga ctgacgcagt ccgggacaac gccccgcacc acaatcacat
     7801 cggctggcac gccctggccg caaagtcgta gcaggaagca tcgccttgcc acatggccgt
     7861 ggaatttgtg tgatattgga atcttccgat tactgcgggc gccggtcaag acattacgtg
     7921 agacggcttc aacattttgt cgggccatcc tgtccgcgct tgaacaggaa gtcgagccgt
     7981 ggttcgacca gtggatggaa cacgatcctt accgcccgcg aggacaggat taccgtgata
     8041 atcaatgcac cgccgaggat catggccttt cccgaaatcg aatcgaactg ctggaaccac
     8101 ttcgattcaa gccacagccg ggcgacaaag ccgtgcagaa ggaatacata catcgtgttg
     8161 cgccctgaag ccgtcaacag tggaatcttg cgtgaaggga ccagtatcag aacggatgcc
     8221 gacagcacgc aggcgacggc atattcacac agccggtaac cggcagcgag ccatccgcca
     8281 atccgcatgc tttcatagga gaatgaaccg taaaaccact ggtgaagcct gtcccggtcg
     8341 cccgtcagga aaaaggcgta cgcggaagcc agaaggatga cggccaggat tctgaacacg
     8401 gcgcccctta ccggaggccg tagcgcgtcc tgaagcacgc ggggcttgaa gaccaccccg
     8461 gcaaggaaga acggcatgaa cacaagggtt cttgacaggc tgccgaagta cccgatgctg
     8521 cccgaatatc cggccgcaag gccgacgaga agcgaaatcg gcaacgccat gcgcaggcgg
     8581 gaaacgtatg gaaggctggc cctccacaga accatgctga acaaaaacca cgtgatccag
     8641 tatggcgaaa acggtgaaaa ggtcagcttc ggcctgtcaa aaagccacca gtcaaacacc
     8701 gaataggcga cctcgaacac aatgtatggg attacaagcc tctcgatgag tttccgttca
     8761 ttccccgcct tcgcgaagtt gcgagagaag tatcccgaaa cgaagacgaa aagcgggatg
     8821 tggaaggagt aaatcaggag ataggccggt ccgtaatcaa gtggttccag catatggccg
     8881 gcgaccacca ggaaaatcag cacaaacttg acgttgtcga accatgcgtc acgttggggc
     8941 gtgtcgcttt tttccgttgc gatgatgtcc aatcctgttc ttccccgagt ttctgttgaa
     9001 gaagttagtc gcaggccccg gtgttttccc gaattgtaat cgtcccgctg cccgccaaga
     9061 gatcgatgct gccttcccag acgacgccgc gatcgaagtc gtcgtcggtc aggtcgcgac
     9121 tcaggcgcac ttcgattgtc gggccgacga cgtcggaaac atcaatcgcg aagtcaaaaa
     9181 ggcacatgca cttgcagcgc ccgctttcat cgtcgggcgc gtcggtctcg gccaggtgat
     9241 agaccccggc gtcgaaaaac gcctggattt cgtgtattcc gcagcagttc aggtcaacgc
     9301 tggaattgat gaaggccagg gtttgtccga ccgcgtcgaa ttgccagttc aggacttcgt
     9361 ccgggcatcc agtctcaccg ctggtcgcgc ttcggggcgc atcgaatccg ccgcaaccgc
     9421 tggcgcgcgg attggttcca atatcgattc cctgattgca ccagcccacg tcttccttga
     9481 tcgtcacgat tccctggccc gttgttgtgt caatccgacc ctcccaggcc accaccgcct
     9541 cgccagtccc ctcgtccgtg accacccgtt caatcctgac gtcaatcgcg tcgccatcga
     9601 tcggggggat ctgaatctgg aaatcgaaca ggcattgaca gaaacagcgt ccttccccgg
     9661 cggcgggtct gtccgtttcc gtgatcacat atacaccgtc ctgcacggtc acctggatgt
     9721 cgtggtcgcc gcagcagtta aggaaaacgt cggtattcgt gaaagtcagc acgccgcttt
     9781 ccgaatcgac atcccactcc agggtttcgt cgccgcagtt cggatcctct tccgccaggc
     9841 gggtttcggg ctggaagccc ccgcattcgc tgtacatcgg gttcaaaccg atattttccg
     9901 gttcggtcgc gtcggtcggc acatcttcgt cgacatccga tccggaatcc gtcgcggaat
     9961 cctggatgac cgcatctgaa gcgccatggt cggcgccgcc cggatcctgg gtccctggat
    10021 cctgagtccc gaggtcggtc gcgacgtcct gaatgcctgt atcctgtcca gaatcggcat
    10081 cggtggttcc cccgccgcat gcaaccagca atgaagcagt cagaaggaac aaaatgaatg
    10141 cagtggattt cacgtcatcc gtcctttggt ggctgtcgca attctatgac tgtgacgggg
    10201 cgttttcaat gcgcccgaat ctggggccac tggaacggga ttgaatcgtg cgggcggtgt
    10261 aattcgaacc gggaaatgca ctccggccat tactgtgccg cgccctggca gacgcctcga
    10321 catcgacctt gattaacccg gcaattgaca tcgcactcgc aaggcgtacc attccgcgtg
    10381 tttgaaacac tgccttgcct gaaaggatcg tgtcaatgaa ggttggtgtc cttacaaatc
    10441 tgaattccag gcgaaacagg gccggcggca gcgacctgcc cgcaaaggga ttgaaagggt
    10501 cgtgcgtcgt tcgaagaacc agcgacattt cggaaatccg ccccgcgctt gaagaattca
    10561 tcgatatggg ctgtgactgc tgggtcgcgg acggcggcga cggaacgctg cactggatgc
    10621 tgaacgaggg ccgcgaggta ctgaagaagc gggggatgtg gaacggcggt tctgcctatc
    10681 cgatgatagt cccgtcgaac agcggaacca tcgatttcgt tgcaagaacc gtcggcatac
    10741 ggggcaccac ggatcaggtc gtgcgaagcc tgcttgacgg tggcggccag gacgacgact
    10801 ttgaaacggt ttgcctggac accatcgacg tccgggggca cgttcatggc gacccggatg
    10861 gaacctgggc cttcgaaagg acaggattcg ccattgccat cggcggaatc ggccagaagt
    10921 ttttcgccaa gtattaccag cgcccccatc gcaacccgct ttcgatcatc gatatcagtg
    10981 cgcgcaccag cgttggatac attgcatccc tgctgcccga gaagctggag tcggtctttc
    11041 cggacgacgt cctggagacc ggaaggtaca tactctcggg aaccagggcc gacgtcgtcg
    11101 cggacgggcg caagttcgac tacggcgtgt accaggggct tcatgtg
//
LOCUS       DAOPPA010000039        11012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6796_length_11012_cov_4.567035, whole genome shotgun sequence.
ACCESSION   DAOPPA010000039 DAOPPA010000000
VERSION     DAOPPA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6796_length_11012_cov_4.567035"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKH54_02195"
     CDS             complement(<1..728)
                     /locus_tag="PKH54_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOC98726.1"
                     /translation="MGDEKDYKATLCLPGTSFPMKANLKEKEPEFLKFWEQNRIYERM
                     MEGAVSAEPFLFHDGPPYANGHIHHGHILNKVLKDIVVKTANMTGHRCENRPGWDCHG
                     LPIEHAVEKVVGREKARANPSELRHRCRDYAREWVDTQRAEFKRLGVLAEWENPYLTM
                     THDYEAKTLREFGRFVGRGDVVLGHKPVFWCHECETALADAEVEYKEATSPSIYVKFP
                     FADSVGERIPALKGRSGGVIIWTTT"
     gene            complement(814..2070)
                     /gene="glyA"
                     /locus_tag="PKH54_02200"
     CDS             complement(814..2070)
                     /gene="glyA"
                     /locus_tag="PKH54_02200"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOC98727.1"
                     /translation="MDQKTLLQSDPEVAALVSSEESRQFEKVRLIASENYTSRAVLEA
                     TGSVFTNKYSEGYAGKRYYEGQQCVDPLEELAISRAKALFGADHANVQAYSGSPANLA
                     VYFATLKPGDTVMGMALPSGGHLTHGWKVSITGSFFRSVQYSVDGTTGLIDYEKVREL
                     ALAERPKLLICGATAYPRILDFAAFRSIADEVGAVLLADMAHIAGLVAGGAHPSPIPH
                     ADVVTTTTHKTLRGPRGAMILCREAMAKAIDKAVFPGLQGGPHNHTTAGIAVALKEAS
                     TPDFKAYAAQVVANAKALAGHLAGHGFKLVSGGTDNHLILMDVTPRGMTGKSYAQALD
                     RAGLECNYNTVPDDPRKPFDPSGLRLGTAAVTSRGMKETEMKFIADCFNRVAGMVEDV
                     DGLDRLAAEVREFCAAYPAPGIISRH"
     gene            complement(2087..3397)
                     /gene="thiC"
                     /locus_tag="PKH54_02205"
     CDS             complement(2087..3397)
                     /gene="thiC"
                     /locus_tag="PKH54_02205"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOC98728.1"
                     /translation="MARRSGFGTQLQAARRQEITPEMEKVSAKESVPAEVIMQRIAAG
                     TVVIPANPAHRNLDPVGIGSGLTVKVNVNLGRSPTSSCLSEEVEKVRSAIRYGADAVM
                     DLSTGPGIDSVRLAVLEASTAPVGTVPVYQAAETVERFEDLSEDDLLGVLSKQAVQGV
                     DFMTIHAGLLRSHIPLASRRRIGIVSRGGALMARWMMAHERENPFYSRFDEVLRIAAE
                     HDVTLSLGDGLRPGCLSDASDEAQFAELDTLGELTRRAWDAGVQVMIEGPGHVPFDQI
                     ESNMKRQKQVCGGAPFYVLGPLVTDIAPGYDHITGAIGATMAAFCGADFLCYVTPKEH
                     LGLPDLEDVRTGLVAFRIAAHAADIARRHPGARDRDDRMSDARFRFDWETQFSLALDP
                     DRAREYHAMTLPAAEHLKSEYCSMCGEKYCAMRNSAIATGRKNR"
     gene            3557..4585
                     /gene="tsaD"
                     /locus_tag="PKH54_02210"
     CDS             3557..4585
                     /gene="tsaD"
                     /locus_tag="PKH54_02210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOC98729.1"
                     /translation="MKVLGIESSCDETSAAVVIDGRQVVSNVVWSQIKVHEPFGGVVP
                     ELASRNHLRYITPVVDSALRKAGIAPSDLDGLAVTIGPGLMGSLLVGVQFAKAMAAAT
                     GTPLVGVNHLEGHLSASFMIDGIEPPEFPYVGLAVSGGHTSIYLVEGWGRLKLLGRTL
                     DDAAGEAFDKAASMLGLSYPGGVVIDRISRGRNVAAVGFPRPMLRESTMNMSFSGLKT
                     ALKRHLETLPATPDADQTAEIAASFQEAVVDILVARTVGAAVSTGVRDVVIAGGVAAN
                     SRLRERMDLACRAADRRLLAVPLSLCTDNAAMIAGLGYQYLFGVLRDAECPRGIDMDP
                     FMRHVSAG"
     gene            4604..6022
                     /locus_tag="PKH54_02215"
     CDS             4604..6022
                     /locus_tag="PKH54_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98730.1"
                     /translation="MRTLTAILLPCLLIACGQQPRIILQDIDESDSVDSGDDTNESEL
                     PDDGLELELPFDATVGDESSGDSSGDTASQLCADSMKPWMCDDRGCTEGFFCNGYFEC
                     DRPPCWGPWCDYVPGTCVPAVSGTVCTAPRDCREGHTCVAGTFSISGICRALPVPGTC
                     WSNDDCEPFGMCAGEVRCHVGNPCLGPEMPGVCVEKINDGRCWDDGMCSSGWCRGAVL
                     CSLDDDGCEPSPGICEAGDGLGCPTTDGGLPCSASDDGNWCVGPENLKYCAMPPASPQ
                     AAGECWTDSDCFPKGGILCRSALTCPPRSFCRLEGMHSGICGQAPPDGQGIELRFVQA
                     TTTGQLQPDSRTQAVLVNRGPVAIFIAPCDTISVMAQRNGEWPGITLDWTFSTPECYT
                     GIPESTLLRIPPGSGAVLSVSTDDAIDQQMAGRNIRLSIRYLLGCVPGKGTDDRMDCL
                     EASNGLARTWFGDPVFWPAGGT"
     gene            6019..6843
                     /gene="rsmA"
                     /locus_tag="PKH54_02220"
     CDS             6019..6843
                     /gene="rsmA"
                     /locus_tag="PKH54_02220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOC98731.1"
                     /translation="MTRRMPDSDGSGLNISQPARKKSLGQHFLNSGRVVSRIIDALAP
                     GPELAVVEIGPGRGALTRELAARCGRLVLVELDDGLIPILGANFPEATIVHHDAATLD
                     MSLVAGGNRAVAVGNLPYNAGGQILFNLLDRNPPFERLVLMFQKEVAQRLCAAPGDRN
                     HGAVSVLVSLLCERRYLFDVPPSKFIPPPAVMSGVVMFLPRLLPADASFLHGKEFFRF
                     VHGLFAQPRKTVANSMMDGLRIGRDDAARILAAAGVDPLSRPNCLTPAQVVDMFKA"
     gene            complement(6868..9429)
                     /gene="gyrA"
                     /locus_tag="PKH54_02225"
     CDS             complement(6868..9429)
                     /gene="gyrA"
                     /locus_tag="PKH54_02225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOC98732.1"
                     /translation="MSDGIGPNVVPVNIEDQMCSSYVDYAMSVIIGRALPDARDGFKP
                     VHRRVLFTMHETKNFHNSPYKKSARIVGDVMGKYHPHGDAAIYDTMVRMAQDFSMRYM
                     LVDGQGNFGSVDGDSPAAMRYTEVRMQRLAEEMLADIEKETVDMVPNYDGSLKEPAVL
                     PSRIPNLLINGSSGIAVGMSTNIPPHNLGEIIDGTIALIRDPDLEVSDLMEFIPAPDF
                     PTGGFILGTRGVREAYETGRGTIKMQARAEVKGDRKGEKPMIVVTEIPYLVNKARLIE
                     RIAELHKEKRIDGISDLRDESDREGMRIVIELKRDADPQVVLNQLYAMTPMQSTFGVI
                     MLAISAGRPRLLTLKDALVEFINHRKDVVTRRCLFELRKAQEREHILLGYKIAIDHLD
                     EIIETIRRSSNPEEARTSLMEKFGLTEIQARAVLDLRLERLTRMERDKILLELEEIRR
                     RIERLREILGSEAILMDVIAGELQEIRDQYIDKRRTEITEDYGEINMEDLIPEEDMVV
                     TVSAQGFIKRTPAAQYRSQRRGGKGSRGMGTREEDFVKDMFVASTHTYVFFFTDKGRA
                     FRIRVFQIPAGTRTAKGRALVNLLNLAADERVAAILPVDDFETPSSFVFATKKGIVKR
                     TDLPLFKNIRRTGLIAIKLDDDDDLIQVRLATESDQVLLFSSEGKSILFKIDDLRSIG
                     RDTRGVRGMSVKGADHVIGMEVLQPGSEILAVTERGYGKRTPVEEYRLQRRGGTGILA
                     MKASRKVGFVIGMRAVTPDQEVILTTDTGTIIRTRVSDIRLIGRVTQGVKIMDLGPDD
                     KLVAIDLVMEAEDDEESDVVITDTRPTASRTLFEDLPVEEEEEPSGEVPAEGDDD"
     gene            complement(9528..>11012)
                     /locus_tag="PKH54_02230"
     CDS             complement(9528..>11012)
                     /locus_tag="PKH54_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98733.1"
                     /translation="CLMYEMVYEKPPFVSTTASTTIKRQVYEKPLPLHLVKPGLPQLD
                     AYEGLLNRLLAKDPTGRPATAAEVMDAVSALRQSTFFDVSLVLEDPRNDYPEPVSRFI
                     EETPFVQPSVSEQLVGGGQTMAFEGLGDAVAQMIRSQEEQPSAAAQAAAEEPMQKTEA
                     FDASFIASVVEKAAAGADIDVAVEEAKTEILRPVGDLPVEDAPKPQSEPVAQQTSVKK
                     KKGHKATREIERKPRPVTDEGLSAVVLPGVEKVSKGPLPSSQTISVRKKPDRKGAGPL
                     IAILVLLILIIAAIVGVLWMKSRKATAVAPEQAAQVEQVVQPAARVDSVPPAPAPEPV
                     PAAEAAAPVPAPAEPAAPPPEPAPAEPAPAAQPEAGKVAETAPVAPAPVVQAGQRQDQ
                     ARVPPPPRQGRRAAQAEQPAQQQPEPAAERAPAAKPEPAPSSPDDGVAQAAGLRAKAR
                     KAGKDGDYAAAISLLKRAKELDPENKLDDKFITAWQKKLDESAK"
BASE COUNT         1950 a         3489 c         3364 g         2209 t
ORIGIN      
        1 gttgtcgtcc agattatgac gccgcccgaa cgccccttca gggccggaat cctttcgccg
       61 acactgtcgg caaacgggaa cttcacgtaa atcgatggcg acgtggcttc cttgtattcg
      121 acttccgcat cggccagcgc ggtttcgcac tcgtggcacc agaagaccgg cttgtgcccc
      181 aggactacgt cgccgcggcc gacaaagcgg ccgaactcac gcaacgtctt cgcctcgtag
      241 tcgtgcgtca tcgtcaggta cgggttttcc cattcggcaa gaacgcctag gcgcttgaac
      301 tcggcccgct gggtatcgac ccactcgcgg gcatagtccc tgcacctgtg gcgcagttcg
      361 gacggattcg cccttgcctt ttcgcgcccg acgacctttt cgacggcatg ctcgatcggc
      421 agcccgtggc agtcccatcc aggcctgttt tcacacctgt gtccggtcat gttggccgtc
      481 ttgacgacga tatccttcaa gaccttgttc aggatgtgcc catgatggat atgcccgttc
      541 gcataaggcg gaccgtcgtg gaaaaggaac ggttccgccg aaacggcgcc ttccatcatc
      601 cgttcgtata tccggttctg ctcccagaat ttaagaaatt cgggttcttt ctctttcagg
      661 ttggccttca tcgggaagga tgttccagga aggcataagg tcgctttgta gtccttttcg
      721 tcgcccattg gcgcccccgt tcaatgcccg tcgggcatgt cagaaaaacc gcgcaaaact
      781 ctagcaatat cgctggaaaa gtcaattacg gggtcagtgc cgggaaatta tgccgggggc
      841 cggataagcc gcgcagaatt ccctgacctc tgcggcgagc ctgtccaggc catcgacatc
      901 ctccaccatt cccgccaccc tgttgaaaca gtcggcgatg aacttcattt ccgtttcctt
      961 cattccgcgg gaagtgaccg ctgccgttcc aaggcgaagg cccgatggat cgaacggctt
     1021 cctcgggtcg tcgggaaccg tgttgtaatt gcattccagc ccggcccgat caagcgcctg
     1081 ggcgtatgac ttgcccgtca tgccacgcgg ggtgacgtcc atcaggatca ggtggttgtc
     1141 cgttcccccc gaaaccagct tgaaaccgtg gccggcaagg tggccggcaa gcgccttcgc
     1201 gttcgcgacg acctgcgcgg cataagcctt gaaatccggc gtcgaagcct ctttcagggc
     1261 aaccgcgatg ccggcagtgg tgtgattgtg cgggccgccc tgcaggccgg gaaagaccgc
     1321 cttgtctatc gccttcgcca tcgcctcgcg gcaaaggatc atggcacccc tggggccgcg
     1381 cagcgtcttg tgcgtggtcg tagtgacgac gtccgcgtgc ggaatcgggc ttggatgcgc
     1441 accaccggcc acgagtccgg cgatatgagc catgtcggca agaagcacgg cgccgacttc
     1501 gtccgcgatg gaacggaacg cggcaaaatc caggatcctg ggatacgccg tggcgccgca
     1561 tatcagcagc ttcggacgtt cggcaagggc cagttcgcga accttttcat aatcaatcag
     1621 gccggttgtg ccatcgaccg aatactggac cgaacggaag aaggaacccg tgatcgagac
     1681 cttccagcca tgcgtcaggt gaccgccgct tggaagtgcc atgcccatca ccgtgtcgcc
     1741 cggcttcagt gtcgcgaaat acaccgcaag atttgccggc gaccctgaat atgcctggac
     1801 attcgcgtga tccgcgccaa aaagcgcctt tgccctggaa atcgccagtt cctcgagcgg
     1861 atcgacgcac tgctggcctt cgtagtacct cttgcccgcg tatccttcag agtatttgtt
     1921 ggtgaacacg ctgccggtcg cttcaagcac ggcccgcgac gtgtagtttt ccgacgctat
     1981 caggcgaacc ttttcaaact ggcgcgactc ctcggatgaa acaagggctg ccacttccgg
     2041 atctgactgc agcaacgtct tctggtccat ttcccgattc ctccttctac ctgttcttcc
     2101 tgccggtcgc gattgcactg ttgcgcatcg cgcagtactt ctcgccacac atcgagcagt
     2161 actccgattt caggtgctcc gcggcaggca aagtcatcgc atgatactcg cgggcacgat
     2221 cgggatccag cgcaaggctg aactgggttt cccagtcaaa ccggaacctt gcatccgaca
     2281 tccgatcatc gcggtcgcgg gcgcccgggt gtcttctggc aatgtccgcc gcatgggcgg
     2341 cgatcctgaa ggcaaccagg cctgtcctga catcttcaag gtcgggaagg ccgaggtgtt
     2401 ccttcggggt cacgtagcac aggaagtccg cgccgcagaa cgcggccatg gttgcgccga
     2461 tcgcgccggt gatatggtcg taccccggcg cgatgtcggt cacaagcggg cccagcacgt
     2521 agaagggcgc cccgccgcaa acctgcttct ggcgcttcat gttcgactcg atctggtcaa
     2581 aaggcacatg ccccggtccc tcgatcatca cctgaacccc ggcgtcccag gcacgcctgg
     2641 tcaactcgcc cagcgtgtcg agttcggcga actgggcctc gtcgctggca tccgaaaggc
     2701 accccggccg aagcccgtcc ccaagtgaaa gcgtgacgtc gtgttccgcc gcgatacgca
     2761 gcacctcgtc aaagcgcgaa taaaacggat tctcgcgttc gtgggccatc atccaccggg
     2821 ccatcagcgc gccgccgcga ctcacgatgc cgattctgcg acgactggcg agcggtatgt
     2881 gcgaccgcag cagaccggcg tggatcgtca tgaaatcgac gccctggacg gcctgcttcg
     2941 aaaggacgcc cagaagatcg tcctcgctga gatcctcgaa cctctcgacg gtctcggcgg
     3001 cctgatagac gggaaccgtt ccgaccggcg ccgtgcttgc ctcaagaacc gcaagacgca
     3061 ccgagtcgat gccgggaccg gtggaaaggt ccatgacggc gtcggcccca tacctgatgg
     3121 cgcttcttac cttttcgacc tcttccgaaa ggcacgaact ggtcggcgac ctgccaaggt
     3181 tcacattgac cttgacggta agcccggaac caattccgac cggatcaagg ttccggtgtg
     3241 ccgggttcgc cggaatgact accgttccgg ccgcaatcct ttgcattatc acttccgcgg
     3301 gaaccgactc cttcgcggaa accttttcca tctcaggggt tatctcttgc cgacgtgctg
     3361 cctgaagctg cgttccgaat ccagatctgc gagccatgaa gaacagtcct ccgaatcccg
     3421 cccgaaccgg ggcaatacaa actaccgccg tttcgtgtcc atcgcaagca gacgaccgcc
     3481 gcgccgaccg gctgctggtt cgaccatgcg acgattggca tgtctgaagc cgcgatattc
     3541 tgctagaatc cgcgccatga aggttcttgg aatagaaagt tcatgtgacg aaacatcggc
     3601 cgccgtcgtg attgacggcc ggcaggtggt ttccaacgtc gtgtggtcac agatcaaggt
     3661 gcatgaaccg ttcggcggcg tcgtgcccga gctggcctcg cgcaaccacc tgcgctacat
     3721 cacgccggtc gttgattcgg cacttcggaa ggccggcatc gcgccttccg atcttgacgg
     3781 gcttgcagtc acgatcgggc cgggcctgat gggctccctc ctggtcggcg ttcagttcgc
     3841 caaggccatg gcggcggcga ccggcacgcc tcttgtcggc gtcaaccacc tcgaggggca
     3901 cctttcggcg tccttcatga ttgacggaat cgagccaccg gagtttccgt acgtcgggct
     3961 ggccgtatcc ggcgggcata caagcatcta cctggtcgaa ggctggggac ggcttaagct
     4021 tcttggacga accctggacg acgccgcggg cgaggcgttc gacaaggccg cttccatgct
     4081 cggcctttcg tacccgggcg gcgtggtaat cgatcgcatc agccgtgggc ggaacgtggc
     4141 cgcggtcggt tttccaaggc cgatgctgcg cgaatcgacc atgaacatga gtttttccgg
     4201 cctgaagacc gcactgaaga ggcacctgga aacgcttccc gcgacacccg atgccgacca
     4261 gaccgcggag atcgccgcat cgttccagga agcggtcgtt gacatcctcg tcgcacggac
     4321 ggtcggggcc gccgtttcga caggcgtgcg ggacgtggta atcgcgggcg gggtcgcggc
     4381 caacagccgt ctgcgcgaaa ggatggatct ggcctgccgg gccgccgacc gacgcctgct
     4441 ggcagtcccg ctgagcctgt gcaccgacaa cgccgcgatg attgccggcc ttggctacca
     4501 ataccttttc ggcgtgctca gggacgccga gtgcccccgc ggaatcgaca tggatccctt
     4561 catgcgccat gtgagcgccg gttaaggcgc cctggaggtt gccgtgcgga ccctgacggc
     4621 catcctgctg ccatgcctcc tgattgcctg cggccagcaa ccacgaatca tcctgcagga
     4681 tatcgatgag tccgattccg tcgattccgg cgacgacacg aatgaatccg aacttccgga
     4741 cgacggcctg gaactcgaac tgcctttcga cgcgaccgtc ggcgacgaat cttccggtga
     4801 ctcttccggt gacaccgcca gccagctctg cgcggattcg atgaaaccct ggatgtgcga
     4861 cgaccggggc tgcacggaag gtttcttctg caacggctat ttcgagtgcg accggcctcc
     4921 atgctggggc ccctggtgcg attacgtccc cgggacatgc gttccggcgg tttccggcac
     4981 cgtctgcacc gcgccgcgcg actgccggga agggcacacc tgcgtggccg gcacgttttc
     5041 gatttcgggc atctgcaggg cgctccccgt gcccggaaca tgctggtcca acgacgactg
     5101 cgaacctttc gggatgtgcg ccggcgaggt tcgctgtcat gtcggcaatc catgcctggg
     5161 cccggaaatg ccgggcgtct gcgttgaaaa gataaacgac ggacgatgct gggacgacgg
     5221 catgtgctca agcggctggt gccggggcgc ggtactgtgc agccttgatg acgacggctg
     5281 cgagccgtca cctggcatct gcgaagccgg ggacggactt ggttgcccga cgaccgacgg
     5341 cgggcttccg tgctcggcgt ccgatgacgg aaactggtgc gtggggcctg aaaacctgaa
     5401 atactgcgcg atgccgccgg ccagtcccca ggcggccggc gaatgctgga ccgactccga
     5461 ctgcttcccg aagggcggga tcctttgcag atcggctctt acatgcccgc cgcgcagttt
     5521 ctgccgcctg gaaggcatgc attccggaat ctgcggacag gcgcccccgg acggtcaggg
     5581 gatcgaactc cggtttgtcc aggcgacgac gaccggacag cttcaaccgg actcccgcac
     5641 ccaggccgtt cttgtcaacc gggggcctgt ggcgattttc atcgcgccct gcgacacgat
     5701 ttccgtcatg gcacaacgca acggggaatg gcccggaatc actctcgact ggaccttctc
     5761 gacccctgaa tgctacacgg gcattcccga atcgacgctt cttcgaatac caccgggatc
     5821 gggggcggta ctatccgtat cgaccgacga cgcgatagac cagcagatgg ctggccggaa
     5881 catccgactg tccatcaggt accttcttgg ctgcgtcccg ggcaagggaa cggatgaccg
     5941 gatggattgc ctcgaggctt ccaacggcct tgcccggaca tggttcggcg acccggtatt
     6001 ctggccggcg gggggcacat gacacgacgc atgcccgaca gcgacggatc cggcctgaac
     6061 atctcccagc ccgcgcgcaa gaaatcgctt ggccagcatt tcctgaacag cggacgcgtg
     6121 gtgtcacgta tcatcgacgc cctggctccc ggccccgaac ttgccgtggt ggaaatcggg
     6181 cccggacggg gcgcgcttac aagggaactt gcggccaggt gtgggcgact ggttctcgtg
     6241 gaacttgatg acggcctgat tcctattctt ggcgcgaact tcccggaagc gaccatcgtc
     6301 caccacgacg ccgcgaccct ggacatgagc ctcgtggccg gcgggaaccg ggccgtggcg
     6361 gtcgggaacc tgccgtacaa cgccggcggc cagatacttt ttaacctgct tgacaggaat
     6421 ccgccgtttg aacgcctggt gctgatgttc cagaaggaag tcgcgcaaag gctgtgcgcg
     6481 gcccccggcg atcggaatca cggggccgtg tcggttctgg tgtcgcttct ttgtgaacgc
     6541 aggtatctgt tcgacgtgcc gccttcaaaa ttcattcctc cgccggccgt gatgtccggg
     6601 gtcgtgatgt tcctgccgcg tttgcttcct gcggacgctt cattcctgca tggaaaggaa
     6661 ttcttccggt tcgttcacgg cctgttcgcc cagccgcgca agaccgtagc aaacagcatg
     6721 atggacgggc tgcgcatcgg tcgcgacgac gccgcgcgga ttcttgccgc ggcgggcgtc
     6781 gatccgctgt cgcggccgaa ctgccttacg ccggcgcagg ttgttgacat gttcaaggct
     6841 tgattgcttc gggcgggcgg gaacgaatca gtcgtcatcc ccttcggcgg gcacttcgcc
     6901 ggacggttcc tcttcttcct cgaccggaag gtcctcgaaa agcgtcctgg aagcggtcgg
     6961 cctggtgtcg gtaatgacga catcggactc ttcatcgtcc tcggcttcca tcaccagatc
     7021 gattgcaacc agcttgtcgt ccggccccag atccatgatc ttcacgccct gggtgacgcg
     7081 gccgataagc ctgatgtcgc tgacccttgt ccggatgatg gttcccgtgt cggtcgtcag
     7141 gatgacttcc tgatccggcg tgaccgcgcg cattccgatg acgaagccga ccttgcggct
     7201 ggccttcatg gccaggatgc cggtaccgcc acgacgctgc agcctgtact cctcgaccgg
     7261 agtgcgcttg ccgtagccgc gttcggtgac ggcaaggatc tcgctgcccg gctgaaggac
     7321 ttccatcccg atgacgtgat ccgcgccctt gaccgacatg ccgcgaacgc cgcgggtatc
     7381 gcgtccgatg ctgcgcagat cgtcgatctt gaacaggatc gacttgcctt cgctggagaa
     7441 cagcagaacc tgatcggatt cggtggccag acgcacctgg atcaggtcgt cgtcgtcgtc
     7501 gagcttgatg gcgatgaggc cggttcgcct gatgttcttg aacaggggca aatcggtccg
     7561 cttgacgata cccttcttcg tcgcgaaaac gaacgatgac ggcgtctcga aatcgtcaac
     7621 tggaagaatc gccgcgaccc tctcgtcggc ggccaggttc aacaggttga ccagcgccct
     7681 gcccttggcg gtcctggtac cggccggtat ctggaagacg cggatcctga acgcgcgtcc
     7741 cttgtcggtg aagaagaaca catacgtgtg cgtcgatgcc acgaacatgt ccttgacgaa
     7801 atcttcctcg cgcgtgccca tgccgcgact gccctttccg ccgcggcgct ggctgcggta
     7861 ctgggcggcg ggcgtgcgct tgatgaatcc ctgggccgat acggtgacga ccatgtcctc
     7921 ttccgggatc aggtcttcca tgttgatctc gccgtaatcc tcggtgatct cggttcgacg
     7981 cttgtcgatg tactggtcgc gtatctcctg gagttcgccg gcgatcacgt ccatcaggat
     8041 cgcctcggat cccaggattt cgcgaagcct ttcgatgcgg cggcggatct cttccagttc
     8101 cagcaggatc ttgtcccgct ccatcctcgt aagccgttca aggcgcagat ccaggacggc
     8161 ccgtgcctgg atttcggtca ggccgaactt ttccatcagg ctggtgcggg cctcttccgg
     8221 gttggaggaa cgacgaatcg tctcgatgat ttcatcaagg tgatcaatcg caatcttgta
     8281 tccgagcagg atgtgctcgc gttcctgggc cttgcgaagt tcaaacaggc aacgcctggt
     8341 gaccacgtcc ttgcggtggt tgatgaactc gacaagcgcg tccttcaggg tcagcagcct
     8401 gggacgcccg gccgatatcg cgagcatgat gacgccgaac gtgctctgca tcggggtcat
     8461 cgcgtaaagc tggttcagga cgacctgggg gtcggcatcg cgcttcagtt cgatgacgat
     8521 ccgcatgcct tcgcggtctg attcgtcacg cagatcggaa atgccgtcga tccgcttttc
     8581 cttgtgcagc tcggcgattc tctcgatcag cctggccttg ttgaccaggt aagggatctc
     8641 ggtaacgacg atcatgggct tttcgccctt gcgatcgccc ttgacctcgg cgcgggcctg
     8701 catcttgatg gttccgcggc cggtctcgta tgcctccctg acgccgcgcg tgcccaggat
     8761 gaacccgccg gtcggaaagt ccggggcggg tatgaattcc atcagatcgg acacttccag
     8821 atccggatcc cggatcaggg caatcgttcc atcgatgatc tcgccaagat tgtgcggtgg
     8881 aatgttggtt gacatgccga ccgcgatgcc ggacgagccg ttgataagca ggttcgggat
     8941 gcgtgacgga agtacggccg gttccttcag ggaaccgtcg tagttcggaa ccatatcgac
     9001 ggtttccttt tcgatgtcgg ccagcatttc ctcggcaaga cgctgcatgc ggacttcggt
     9061 gtaacgcatc gccgcaggcg agtcgccatc gaccgacccg aaattgccct ggccgtcaac
     9121 cagcatgtaa cgcatcgaaa agtcctgcgc catgcggacc atcgtgtcgt aaatcgccgc
     9181 gtcgccgtgc ggatggtact tacccatgac gtcgccgacg atgcgggcgc tcttcttgta
     9241 cggcgaattg tggaagttct tcgtctcgtg catcgtgaaa agcacgcggc ggtgaacagg
     9301 cttgaatccg tcgcgggcat ccggcagggc gcgcccgatg attacgctca tcgcgtaatc
     9361 gacgtacgac gaacacatct ggtcttcgat attgactggg accacgtttg gtccgatgcc
     9421 gtcagacatg gggacctcca gaaaagtcga ggaattttac tacgaatcaa gcggcaaggc
     9481 aacgattgaa accggtttca ccggcaggaa aagccaaaca aaaccaacta cttggccgac
     9541 tcatccaact tcttctgcca agccgtgatg aacttgtcgt ccagcttgtt ttcaggatcg
     9601 agttcctttg ccctcttgag cagggaaatg gcggcagcat agtcgccatc cttgccggcc
     9661 ttcctggcct tcgcacgcag ccccgcggcc tgcgcgacgc catcgtcagg acttgacggc
     9721 gccggctcgg gcttcgcggc cggagccctt tccgccgcag gctcgggctg ctgctgggcg
     9781 ggctgttccg cctgggccgc gcgacgcccc tgccttggcg gcgggggcac cctggcctga
     9841 tcctgacgct gtccagcctg gactaccggg gccggcgcca caggggccgt ttcggcgacc
     9901 ttgcccgcct cgggctgggc agccggggcc ggttcggcag gggcgggttc cggcggaggc
     9961 gcggccggtt cggccggcgc gggaacgggc gcggcggcct ccgcggcggg cacgggttcg
    10021 ggcgccggcg ccggcggtac ggaatcgaca cgcgcggccg gctggacgac ctgttcaacc
    10081 tgggcggcct gttcgggcgc gacggccgtt gccttgcgcg acttcatcca caacacgccg
    10141 acgattgccg cgatgatcag gatcaggaga acaagtatcg cgataagcgg cccggcacct
    10201 ttcctgtccg gcttcttcct gaccgagatg gtctgggaag acggaagggg ccccttggaa
    10261 accttttcca cgccgggcag caccacggcg gacaggcctt cgtctgtcac cgggcggggc
    10321 ttgcgctcga tctcgcgggt tgccttgtgt cccttcttct tcttgaccga agtctgctgc
    10381 gcgacaggct cggactgggg cttcggggca tcttcgactg gaagatcgcc gaccgggcgc
    10441 agaatttcgg tcttggcttc ctcgacggcg acatcgatgt cggcaccggc cgcggccttc
    10501 tcgacgacgg aagcgatgaa ggacgcgtcg aacgcctcgg tcttctgcat cggttcctcg
    10561 gcagccgcct gtgccgccgc ggaaggctgc tcctcctgcg agcgaatcat ctgggcaacc
    10621 gcatccccaa gaccctcgaa cgccatcgtc tggccaccgc cgacaagctg ttcggaaacc
    10681 gaaggctgaa cgaaaggcgt ttcctcgatg aagcgactga ccggttcggg atagtcgttc
    10741 ctgggatcct caaggacaag cgacacgtca aagaaggtgc tctgccgcaa ggcggaaacg
    10801 gcatccatta cctcggcggc ggttgccgga cgacctgtcg gatccttcgc cagaagccgg
    10861 ttcagcaacc cctcataggc gtcaagctgg ggcaggcccg gcttgaccag gtgaagcggc
    10921 agcggctttt cataaacctg gcgcttgatg gtggtggaag ccgtcgtcga tacgaacggg
    10981 ggcttctcgt aaaccatctc gtacatcagg ca
//
LOCUS       DAOPPA010000040        10965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_6834_length_10965_cov_3.774335, whole genome shotgun sequence.
ACCESSION   DAOPPA010000040 DAOPPA010000000
VERSION     DAOPPA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_10965_cov_3.774335"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKH54_02235"
     CDS             complement(<1..391)
                     /locus_tag="PKH54_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98734.1"
                     /translation="MDDLEGHPVPDSPERKGADGSHTAEFLVQGSAAIRNNLRLLAAS
                     SVILAGLAIGYTMWALSSVMVPLVLAFVLSYLVSPVARFFRDRLHLGHVIGVVLAMLV
                     ATAVMGLVGYIIFYSVSEAIEGWPAYQD"
     gene            553..2280
                     /locus_tag="PKH54_02240"
     CDS             553..2280
                     /locus_tag="PKH54_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOC98735.1"
                     /translation="METLNNVLSLASDLVWNTPSVLPAMVVLLLFSGLFLTVRLGFIQ
                     FRRLGHAIRVVAGKYDNNSSSGDVSHYQALTSALSATVGIGNIAGVATAIHYGGPGAV
                     FWLWLTGFLGMATKFVECTLAQKHRVIHPDGSVSGGPMYYIEKGLGPRWKWVAILFAV
                     CAATGAMGTGNSIQAFTMADSLRADFGIPNWVSGLVSASIIGLVIVGGIRRIGTVTGK
                     LVPFMGLLYVVSALVVVALNIDRLPGAVSDIVTYAFSPPGMIGGFAGSTFVFTLTWGV
                     KRGLFSNEAGQGSAPIAHAAAKTDRPVSEGSVALLEPFVDTVVICFITGLVIVTMGTW
                     NQKHPDRFDITAKSGLTWVKTGTDVMERGQVDAESVFAGTSRITDGLAETVLVVRNNS
                     IVDQARFHLDGKPFSGEITLSGGVPIARDNNGKTIPASRVFITGNALLNGSPLTATAF
                     RDGLMPVFPWGNYLITIVVFLFAISTAISWSYYGDRAILYLAGPRAVLPYKVLFTAVH
                     FIGAIVSLEAVWAFGDTALGLMAIPNLIAVLILSRKVRKESDAYFAQVDADEMAQRRE
                     HKRKRPRES"
     gene            2292..3839
                     /locus_tag="PKH54_02245"
     CDS             2292..3839
                     /locus_tag="PKH54_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOC98736.1"
                     /translation="MNAPEQNGCDDAGRGQFKSRLGFVLAAAGSAVGLGNLWRFPYLT
                     YKYGGAGEEGGGAGTFVLVYLLCIALVCLPLILAEILVGKRGRRNPVGSFNAIRPGTR
                     WNIVGYMGIATGFLILSYYAVVAGWSIEYTYKSVINEFAGFETAISDSEAAQALGAKL
                     TSERRGIGEARAGDSVWVVDGNPEEFKAKLIAKKAELYPQHLFSQFVANPVKQVGYFL
                     AFMVLTVILVLGGVAGGIERGNKIMMPLLLLMLVVLAIRVLTLDGGGRIIGYLFKPSI
                     STLSWDLVLGAMGQAFFSLSVGMGALLTYGSYLGPKDRIDTSSAAIPAIDSSVALLAS
                     IVVCGAIISFGLAMTGSGTGNVFTAIPVIFQSIPGGRWLVVLFYLLVTIAALTSTISL
                     LEVVASYMIDERKMKRTEAVLISAAAISAVGILCALSFNLLAGYTLFNMTFFDLLDYL
                     CSNILLPVGGILIAVFVGWVMRHDEKRQELACMNPIVYRIWNVLLKFLAPAAILAVLV
                     GQFIFRA"
     gene            complement(3898..4275)
                     /locus_tag="PKH54_02250"
     CDS             complement(3898..4275)
                     /locus_tag="PKH54_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98737.1"
                     /translation="MGNRGNRDDRFGMHGGRHGDWPGKPCGDMRDDWPGRPRQDDVEG
                     YGCPEFGFRRHFITRAEKLEALNDYLEELENEAEGVREAIDELLDEMIEAGDFDDDDF
                     EDDDDDGIDGCECGCCDEPEDCK"
     gene            complement(4383..4453)
                     /locus_tag="PKH54_02255"
     tRNA            complement(4383..4453)
                     /locus_tag="PKH54_02255"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4419..4421),aa:Cys,seq:gca)
     gene            complement(4584..10910)
                     /locus_tag="PKH54_02260"
     CDS             complement(4584..10910)
                     /locus_tag="PKH54_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOC98738.1"
                     /translation="MFQSNVEGEMPAATVDACAWTRIYMCYFDEDAYSQTWSFHGYPV
                     SYLDGAPLDQAPFVTGAQDVGAYGFCDTLPIAVQSVCNTPGNINGYYYCGIDIKPNCN
                     EDYAGAYRAGLVSMDEYDRTGVAELHSPFESLTKFDLTVLLEDGYIIWSPNGVADPSA
                     VAIKTAIKAAGRRAQIIKDLSMVSDLTKYDGVFAVLGVKGRYTALSPMNAARLRLFLD
                     QGGKVYLEGGDFFYNAAGSQAPTVLHPYFKAQATSDGGTMRMAGPIEGKNFLHGATFN
                     LLDLATVNSWIDKLVHTPEAGGREIFRNANPADPFAVAVSYEQEVAPSVKYRTIASSL
                     PFGALVESGTTKAQLMALYLNFLENGYPPCTAVENCEDFEVCTTDYCSANVCANLPIT
                     GCVPCKDDRYRPDGSPSCGIDQACDVSVGYCVDIPGFRWDAECDTDFGAAPVSATCTI
                     NVGANGVMDSSNFKVAMTHEYRGDVRLTVTSPAGTTARLRSESMLDSKRTVYETYDIG
                     VVPAEPVTIFDGEPSAGTWTVVAQDMDPTLANGNLEGWNLFGELSDVACTDSSDCPQS
                     LCQVASCTDSICVFTPRNCNDERSCTIDSCNPTTGECEYEVIQACGGPCMTHDDCFRN
                     EVCLVSGSEDRVCDPSSDIDPVSGEALCVCRAIEGQPYEYNWQTDLPAAIPDDEGATP
                     LVKVLNVNASGYVNKLKVKVKTTHEAAGDLRVELCHEGVCVRLRDAKGGTNPGFHDVY
                     EWDPVSGPGAIDNFRRLPVRGDWTLKVWDRVSEKVGSLVYFNIYVVAAQCFNNAACDD
                     NNLCTIDACLHPNEGGTCAHQAKQCEPSDNPCVANVCEPTTGVCGPKTAADGAPCDDG
                     LFCTEDDFCRAGACTAGDPKVCTHLDGNCTVGECSEDLDQCIVVPAVDGAACNDGEPC
                     NAGDFCEAGACQPGPTLVCGCPSGQNSACLDDGNKCNGVNWICNADKMCELADDPVEC
                     PELGIPCRSNVCDPFDGACKMRSALNYVPCEDGLYCSVADYCLSGECMGGGARDCTAL
                     DQDCKTGYCDEDANNCLPINKDDGTVCEKDGYGCSNDACLDGTCQFKQVVDCSDTGDE
                     CNEGICQNMGWGGYVCAKGAFPDGTPCGDEPNPCTEDTCQLGICEHDLLMNCNGPCGG
                     EHPFDAGDDDCGIEDSCDGGIAGNGQGGCTPTCPDGMCYNASSGNIDMTINERAGVGG
                     CISSSVPVSTGFAFVSNLELKLKLEHEALGDLQINLIDPQGARHRVWHNLGEDNNGFA
                     NTFDLSFPVPYPGIETSGQPMCALKGEQIDGIWTLQVCDTAEGNSGVLQKWSLYLKGS
                     DDVNLNKGHRCADAIDLGNQDVNPAIFVDGTLECALDTVNDSGCGGVTGPDRMYKFNL
                     TTKKRVTIKLLPVPGHRLILFMKGADNGTCAAGSMRCGETTDDQPSVIDMQLDPGEYF
                     VGVDSNDGMFGYTPFRFELRVKTLLDNGADCDDLVLGEQDLDCGSLHCQNGFCCDEGD
                     CCPSEAWEFGGDGFDPNELKLDPDWIVTDAVCPVQYKEDAICNDPDVTDPQNPINFCQ
                     GQRWDARCVNNQCVKTEVADDTACDDTVESDRCGFYHSENCGDDGPLPPAVQTKPDCL
                     TYCTSDTDCDPNAHCDPLVATDPDPAPELHQMYCVADLPNGAASNEDSDCASGHSQNG
                     FCCRAGDCCPTDDQFGAMACPGQYTTPASCDTVRDPLTGEMVCEGHRRDPVCVDNICG
                     DEQVRDDCRCGGSIADNCGAFLPARCPVSPTTDCPGAEWGVEWISGGTECLTSCLTNG
                     VPDDSLCDDDARCDWDEDDPTNAICIPLVPNGGPCLEDLECLNQQWLPGGIGHCQNGF
                     CCDFGDCCSRRNDCPTETPRGDYWGSPVCSDFSTCQGLEKLATCNDFRCGSEYLPEDT
                     ACVFDIGQPSDECGFYKPVFCNGQVDQIDPPCPTTCLEAGTGVELDSLCDDNAHCDPV
                     GPTYTNAVCMADLANDQPCDEDSDCVSTFCQAGYCCDIGGCCAGCKVTNATPSFGNAG
                     YEGAFSTEFGYVQFAPSVPLAERPATGGRTVGTELGTFESVTVVTGCWDRIQGQSETD
                     VDCGGRVCPKCAAGKKCVYNRDCLSDNCNAGTCL"
BASE COUNT         2155 a         3315 c         3223 g         2272 t
ORIGIN      
        1 gatcctgata agctggccag ccttcgatcg cctcggaaac cgaatagaag atgatgtacc
       61 cgaccagccc cattaccgcg gtagcgacaa gcatggcaag aactacgccg ataacatggc
      121 ccaggtgcag gcgatcccgg aagaacctgg caaccggcga aacaaggtac gaaagcacga
      181 aggccagcac aagcggcacc atcaccgagg aaagggccca catcgtgtat ccgatcgcaa
      241 gaccggccag gatgacggac gatgccgcga gaagcctgag attgttcctg atggccgcac
      301 tgccctgaac gagaaactcg gccgtgtggc tgccgtcggc tccctttctt tcgggactgt
      361 ccgggacggg gtgaccttcc agatcgtcca tatttcacct tgatggaaag gattcggtcg
      421 aaccggaacg attatagcct cttggcaggc atcgtgacag tcggcacaag gctgctggtc
      481 cttctggact caacttcaag atgatgcata atcgtcctga aaacacttca aggccaatgg
      541 ccgaagacgc ggatggaaac actcaacaac gttctctcac tcgcctccga cctcgtgtgg
      601 aataccccgt cggttcttcc ggcgatggtg gttctgctgc ttttttccgg cctcttcctg
      661 accgtaaggc tgggcttcat ccagttcagg cggctcgggc acgcaatacg ggttgtcgcc
      721 ggcaagtatg acaacaactc gtcgtcaggc gacgtatccc actaccaggc cctgacctcg
      781 gcactgtcgg ccaccgtcgg catcggtaac atcgccggtg tcgcgacggc aatccattac
      841 ggaggacctg gtgccgtctt ctggctgtgg ctgaccggct tccttgggat ggcgaccaaa
      901 ttcgtggaat gcacgctggc gcagaagcac cgggtcatcc acccggacgg atcggtttcg
      961 ggcggaccga tgtactacat cgaaaagggc ctcggtccaa gatggaagtg ggtagccatc
     1021 ctgttcgcgg tctgcgccgc caccggggcc atgggcacag gaaactcgat acaggcattc
     1081 acgatggccg acagcctgcg ggcggatttc ggcatcccga actgggtgtc cggcctggta
     1141 tccgcatcga ttatcggact ggtcatcgtc ggcggcatcc gacgaatcgg gacggtcacc
     1201 ggcaagctcg tgcctttcat gggccttctc tacgtggtaa gcgcgctggt cgtggtcgcc
     1261 ttgaacatcg atcgcctgcc cggtgcggtt tccgacatcg tcacctacgc gttttcgcca
     1321 cctggaatga tcggcggatt cgcaggatcc acgttcgtct tcacgctcac atggggcgtc
     1381 aagcgcgggc ttttttccaa cgaggccgga cagggcagcg ccccgatcgc ccatgctgcc
     1441 gcaaaaaccg accggccggt aagcgaaggt tctgtcgcgc tgcttgagcc cttcgtcgat
     1501 accgttgtca tctgcttcat caccggtctt gtaatcgtca cgatgggaac ctggaaccag
     1561 aaacaccccg atcgtttcga catcaccgcg aaaagcggac ttacgtgggt caagaccggc
     1621 acggacgtca tggaacgcgg ccaggtcgat gccgaatcgg ttttcgcagg gacttcaagg
     1681 ataacggacg gactggcgga aacggttctg gtcgtccgga acaactcgat tgttgatcag
     1741 gcaagattcc atcttgacgg caagccattc tccggtgaaa tcaccttgtc cggcggcgtc
     1801 ccgatcgcac gcgacaacaa cggcaagaca atcccggcat caagggtctt catcaccggg
     1861 aatgcccttc ttaacggttc gccgcttacg gcgaccgcat tccgggacgg gctgatgccg
     1921 gtatttccgt ggggaaacta cctgattacg attgtggtct ttcttttcgc catatcgacc
     1981 gccatttcgt ggagctacta cggcgacagg gcaatccttt acctggctgg ccccagggcc
     2041 gtccttccgt acaaggtgct tttcacggcg gtgcatttca tcggcgcgat cgtttccctg
     2101 gaggccgttt gggccttcgg ggatacggca ctggggctga tggcgatacc aaatcttatc
     2161 gcggttctga tcctttcgcg caaggtcagg aaggaatccg acgcgtactt cgcccaggtc
     2221 gatgccgatg aaatggcaca gcgccgcgag cacaaaagga aaagaccaag ggaatcataa
     2281 gggagtcggg aatgaatgct ccagagcaga atggatgcga cgatgccggt cgcggacagt
     2341 tcaaaagcag gctcgggttc gtccttgcgg ccgcgggaag cgcggtcggc cttggcaacc
     2401 tgtggcgctt tccatacctg acttacaagt acggcggggc cggggaggag ggaggcggcg
     2461 ccggcacctt cgtgctggtt tacctgctct gcatcgcact ggtctgcctt ccgctgatac
     2521 ttgcggaaat ccttgtggga aagcgtggac gccgcaaccc ggtcggttcc ttcaacgcaa
     2581 tccgcccggg cacaaggtgg aacatcgtcg gctatatggg gattgcgaca ggcttcctga
     2641 tcctgtcgta ttacgcggtc gtggcgggat ggtcgatcga atacacatac aagtcggtga
     2701 tcaatgagtt tgccggcttc gagacggcca tttcggacag cgaggcggcc caggcgcttg
     2761 gtgccaagct cacatcggaa cgccggggta tcggcgaagc cagggcgggt gattcggtct
     2821 gggtcgtgga tggaaacccg gaagagttca aggcgaaact gatcgccaaa aaggcggaac
     2881 tgtacccgca gcatcttttt tcccagttcg tggccaaccc cgtcaagcag gtgggttact
     2941 tcctggcatt catggtactg acggtcatcc ttgtgctggg tggcgtggcc gggggaatcg
     3001 agcgcggcaa caagataatg atgcctctgc tgctgctgat gctggtggtg ctggcaatcc
     3061 gggttctgac cctggatggc ggcggtcgca tcatcggata ccttttcaag cccagcattt
     3121 caacactttc atgggatctt gtgctggggg ccatgggaca ggcgttcttc tcgctttcag
     3181 tgggcatggg cgcgctgctt acgtatggaa gctacctcgg gcccaaagac cgcatcgaca
     3241 cctcatctgc cgcgattccg gcgatcgatt cgtcggtcgc cctgcttgca agcatcgtcg
     3301 tctgcggcgc aatcatttcc ttcggccttg cgatgacggg atccggcaca ggtaacgtct
     3361 tcacggccat tccggttatc ttccagagca ttcccggcgg caggtggctg gttgtgcttt
     3421 tctaccttct cgtgaccatc gcggccctta catcaactat atccctgctg gaagtggtcg
     3481 cttcgtacat gatcgacgag aggaagatga aacgcactga agctgtgctc atttccgctg
     3541 ccgcgatttc cgcggtcggc attctgtgcg cgctttcgtt caacctgctc gccggataca
     3601 cgctgttcaa catgaccttc ttcgaccttc tggattacct gtgttccaac atccttcttc
     3661 cggtcggcgg gatactgatc gcggtattcg tgggctgggt gatgcgtcac gacgaaaaac
     3721 gccaggaact tgcatgcatg aacccgattg tctatcggat ctggaatgtg ctgctgaagt
     3781 tcctcgcgcc cgccgccata ctggccgttc ttgtgggcca gttcatcttc agggcttgaa
     3841 ccccggacag gcctgaatca gttcattggt ggtgaaggaa gctggggccc ggacagccta
     3901 cttgcaatcc tcgggttcat cgcagcagcc gcattcgcag ccgtcgatgc catcgtcatc
     3961 gtcatcctcg aagtcatcgt catcgaagtc accggcctcg atcatctcgt ccagcaattc
     4021 atcgattgcc tcgcgcacgc cctcggcctc gttttcaagt tcctcgaggt agtcgttcag
     4081 tgcctcaagc ttctcggccc ttgtaatgaa atgtcttctg aagccgaact cgggacagcc
     4141 gtatccctcg acatcatcct ggcgcggcct tcccggccag tcgtcacgca tatcgccaca
     4201 tggcttcccc ggccagtccc catgacgccc accgtgcatc ccgaaacgat catccctgtt
     4261 tccccggtta cccatgattt cctccacaat tgttgcgatc ccggcacgac tatattgcca
     4321 cgccgacgcc gtgtcaaaac acttcgacct gacaagatga tcgcatgagt atttgagtct
     4381 tgaggcgcca cccggatttg aaccgggggt ggagattttg cagacctctg ccttaccact
     4441 tggctatggc gccacttcaa aagcaggacg attctacagg ccacggcatt gccgtgtcaa
     4501 ggttcgattc cagtcgttga tttcaggaaa tgcccgatgt ccggcccctt aaccggcaag
     4561 gggccggacc cgggaaaaaa tcactacagg caggtaccgg cgttgcagtt gtccgacagg
     4621 caatcgcggt tataaacgca cttcttgccg gcagcgcact tcgggcagac acgaccgccg
     4681 cagtcaacgt cggtctctga ctggccctgg atcctgtccc agcatccggt cacaacggtg
     4741 acgctttcga aggtgccaag ttccgtgccg acagtacgac cgccggttgc aggcctttcg
     4801 gccagcggga cgctcggggc gaactgcacg taaccgaact cggtcgagaa cgcgccttcg
     4861 tacccggcgt tgccgaacga cggggtcgcg ttcgtgacct tgcagccggc gcagcagccg
     4921 ccaatgtcgc agcagtaacc agcctgacag aaggtgctga cgcagtccga atcctcgtca
     4981 cacggctgat cattggccag gtccgccatg cataccgcgt tggtgtaagt cggtccaacc
     5041 ggatcgcagt gcgcgttgtc gtcgcacaga ctgtccagtt ccacgccggt tccagcttca
     5101 aggcaggtgg tcggacacgg aggatcaatc tgatcgacct ggccgttaca gaacaccggc
     5161 ttgtagaagc cgcattcatc ggaaggctgt ccgatgtcga acacgcaggc ggtgtcctcg
     5221 ggcaggtatt cggatccgca cctgaagtca ttgcatgtcg caagcttttc aagaccctgg
     5281 caggtcgaga agtcgctgca gaccggggaa ccccagtaat cgccacgcgg ggtttcggtc
     5341 gggcagtcgt tcctgcggct gcaacaatcg ccgaagtcgc agcagaagcc gttctggcaa
     5401 tgtccgatgc caccgggcag ccactgctga ttcaggcatt ccagatcttc caggcacggc
     5461 ccgccgttgg gcaccagcgg gatacagatt gcgttggtcg gatcatcctc gtcccaatcg
     5521 caacgtgcat cgtcatcgca cagactgtcg tccggcacgc cattcgtcaa acaggacgtc
     5581 agacactcgg ttccgccgga aatccattcg acaccccatt ccgcaccggg gcagtccgtg
     5641 gtcggggaca ccgggcaacg ggccggcagg aacgcgccgc agttgtcggc aatcgaaccg
     5701 ccgcagcggc agtcgtcacg aacctgctcg tcaccacaga tgttatcgac gcacacagga
     5761 tcgcgcctgt ggccttcaca gaccatttcg ccggttaacg gatccctgac agtgtcgcac
     5821 gaagccggag tcgtgtactg accggggcac gccatggcac cgaactggtc gtcggtcggg
     5881 cagcagtcgc ctgccctgca gcagaagccg ttctggctgt gaccactcgc gcagtcgctg
     5941 tcctcgttgg aagccgcacc gttcggaaga tcggccacgc agtacatctg atgaagttcc
     6001 ggcgccggat cgggatcggt tgccaccagc gggtcgcagt gcgcgttcgg gtcgcagtcg
     6061 gtatccgacg tgcaataagt cagacagtct ggcttggtct gcacggccgg cggcagagga
     6121 ccgtcatcac cgcaattttc cgagtgatag aagccgcagc gatccgattc gacggtgtcg
     6181 tcgcaggctg tgtcgtcagc cacttcggtc ttgacgcact gattattgac acaccttgcg
     6241 tcccagcgct gtccctggca gaaattgatt gggttctgcg gatccgtcac gtccggatca
     6301 ttgcagatcg catcttcctt gtactgtacc gggcagacag catcggtcac gatccagtcc
     6361 ggatccagct tcaattcgtt cgggtcaaag ccgtcgccac cgaactccca ggcctcggac
     6421 ggacagcagt caccttcatc gcagcagaat ccgttctggc agtgcaggct gccgcagtca
     6481 agatcctgtt caccaagaac gaggtcatca caatccgcac cattgtcaag cagggtcttg
     6541 acgcgcagtt cgaaacggaa cggggtgtat ccgaacatgc catcgttcga atcgacgccg
     6601 acgaagtact cgcctggatc aagctgcatg tcgatgacac tcggctgatc atcagtagtt
     6661 tcgccgcacc gcatcgagcc ggcggcacag gtgccattgt ccgcaccctt catgaacaga
     6721 atcagcctgt gtcccggaac cgggagcaac ttgatcgtga cgcgcttctt cgtcgtcaga
     6781 ttgaacttgt acatgcgatc cgggccggtc acgccgccac aaccggaatc attgacggta
     6841 tccagcgcgc attcaagcgt tccatcaaca aagatggccg ggttaacgtc ctgattgccc
     6901 agatctattg cgtcggcaca acggtgtccc ttgttcaggt tgacatcgtc cgagcccttc
     6961 aggtaaagcg accacttctg aaggacgccg ctgttgcctt cggccgtgtc gcagacctgt
     7021 aaggtccaga tgccgtcgat ctgctcgccc ttcagggcgc acatcggctg accggatgtt
     7081 tcgattcccg ggtacggaac cgggaaggaa agatcgaatg tgttggcaaa accgttattg
     7141 tcctcaccaa gattgtgcca gacgcggtgc cttgcaccct ggggatcgat caggttgatc
     7201 tgaagatcgc ccagagcttc gtgttccagc ttcagcttca gttcaaggtt gctgacaaaa
     7261 gcgaaaccgg tgctgaccgg aaccgagctg ctgatacaac cgccgacgcc ggcgcgctcg
     7321 ttgatggtca tgtcgatatt gccggaagac gcgttgtagc acatgccgtc cgggcacgtg
     7381 ggtgtacagc cgccctgccc atttccggca atgccgccgt cgcaggagtc ttcgatgccg
     7441 cagtcgtcgt cgcccgcgtc aaacgggtgt tcgccaccac agggtccgtt gcagttcatc
     7501 agaaggtcat gttcgcagat accgagctgg caggtgtcct cggtgcatgg attgggttca
     7561 tcgccgcagg gagtcccgtc agggaaggcg cccttcgcgc agacgtatcc gccccagccc
     7621 atgttctggc agatgccttc attgcattcg tcgccggtat ccgagcaatc aacgacctgc
     7681 ttgaactgac aggttccgtc cagacacgcg tcattcgaac atccgtatcc atccttttca
     7741 cagacggtgc cgtcgtcctt gttgatcggc aggcagttat tcgcgtcttc gtcgcagtat
     7801 ccggtcttgc aatcctggtc gagggccgtg caatcacggg cgccgccgcc catgcattca
     7861 ccggacagac agtaatccgc aacgctgcag tacagtccgt cctcgcatgg cacgtagttc
     7921 agggcgctgc gcatcttgca tgccccatcg aacgggtcac aaacgttgct gcggcacggg
     7981 atgccgagtt ccgggcattc gaccggatcg tcggccagtt cgcacatctt gtcggcgtta
     8041 cagatccagt tcacgccgtt gcatttgttg ccgtcgtcaa ggcatgctga attctggccg
     8101 cttgggcacc cgcagaccag cgtcgggcct ggctggcagg caccggcctc gcagaagtcg
     8161 ccggcattgc acggttcgcc atcgttgcag gcagcgccgt caaccgccgg caccacgatg
     8221 cactgatcaa gatcctcgga gcactccccg acggtgcagt tgccgtcaag gtgggtgcaa
     8281 actttcggat caccggccgt acaggcgccg gcacggcaga aatcgtcctc ggtgcagaac
     8341 aggccgtcgt cgcaaggtgc tccgtcggcg gcggtcttgg gaccgcagac accggtggtc
     8401 ggttcacaga cattggccac gcacggattg tccgaaggtt cgcactgctt ggcctggtgg
     8461 gcgcacgtgc caccctcgtt cggatgcagg catgcatcga tggtacacag gttgttgtcg
     8521 tcgcatgccg cattgttgaa gcactgcgcg gcgaccacat agatgttgaa atacaccagg
     8581 gagccgacct tttcagagac acggtcccag accttgaggg tccagtcacc gcgcacgggc
     8641 agacgccgga agttatcaat cgcgccgggg cccgacacag gatcccactc ataaacatcg
     8701 tggaatcccg ggtttgttcc acccttggcg tcgcgcaggc ggacacagac gccttcgtgg
     8761 cacagctcaa cgcgcaggtc accggcggcc tcgtgggtcg tcttgacctt gaccttcagc
     8821 ttgttgacat agccgctggc gttgacgttc aggaccttga ccagcggggt cgcgccttcg
     8881 tcgtccggaa tcgccgccgg cagatcggtc tgccagttgt attcataggg ctggccctcg
     8941 atagcgcggc atacgcaaag ggcttcaccc gagacaggat cgatgtctga tgacggatcg
     9001 cataccctgt cctcggatcc cgacacaagg cagacctcgt tgcggaagca gtcgtcgtgt
     9061 gtcatgcacg gaccgccgca ggcctggatc acctcgtatt cgcattcccc ggttgtcggg
     9121 ttgcacgaat cgatggtgca tgagcgttcg tcgttgcaat tccttggtgt aaagacacag
     9181 atgctgtcgg tgcaggaagc gacctggcac aacgactgcg ggcaatccga actgtcggtg
     9241 catgccacgt cggacagttc gccgaacaga ttccatcctt caagattgcc gttcgcaagg
     9301 gtcggatcca tgtcctgggc gacaacggtc cacgttccgg ctgaaggttc gccgtcgaaa
     9361 atggtcaccg gttcggccgg aacgacgccg atatcgtacg tttcataaac cgtccgcttg
     9421 gaatcgagca tcgactcgga acgaagcctg gcggtcgtgc cggccgggct ggtaacggtc
     9481 aggcggacat cgccgcggta ttcatgggtc atcgcgacct tgaaattgga actgtccatg
     9541 acgccgttgg caccgacatt gatggtacat gtggcggaaa ccggcgcggc gccgaaatcg
     9601 gtgtcgcact ccgcgtccca acggaagccg gggatatcga cgcagtaccc gacgctgacg
     9661 tcacaagcct gatcgattcc gcacgacggg gatccatccg ggcgatacct gtcgtccttg
     9721 cacgggacgc agccggtaat cggcaggttc gcgcaaacgt tggccgagca gtagtcggtg
     9781 gtgcagactt cgaaatcctc gcaattctcg acggcggtgc acggcggata accgttttca
     9841 aggaagttca ggtacagcgc catcaactgg gcctttgtcg ttccggactc caccagcgcg
     9901 ccaaacggca acgaagaggc gatggtccgg tacttcactg acggggcgac ttcctgttcg
     9961 taagaaacgg ccacggcaaa gggatcagcc ggattcgcgt tgcggaaaat ttcccgcccg
    10021 ccggcttccg gtgtgtgaac cagcttgtcg atccaggaat tgaccgttgc cagatcaaga
    10081 aggttgaacg ttgctccgtg caggaagttc ttgccctcga tcgggccggc catgcgcatg
    10141 gtgccgccat ccgacgtggc ctgggccttg aagtacggat gaagaacggt cggggcctgg
    10201 cttccggcag cgttgtagaa gaagtcgccg ccctcgagat agaccttgcc accctgatcc
    10261 aggaacaggc gcagccgcgc cgcgttcatc ggggaaagcg ccgtgtagcg gcccttgacg
    10321 ccaagcacgg caaacacgcc gtcgtacttg gtgagatccg aaaccatcga cagatccttg
    10381 atgatctggg ccctgcggcc ggcagccttg atggccgtct tgatcgcgac ggcgcttgga
    10441 tcagccacgc cgttcggcga ccagatgatg tatccgtctt ccagaaggac cgtcagatcg
    10501 aacttcgtca aggactcgaa cgggctgtga agttcggcca ctccggtcct gtcgtattcg
    10561 tccatggaaa ccaggccggc cctgtaggcg ccggcgtagt cttcgttgca gttcggcttt
    10621 atgtcgatac cacagtagta atacccgttg atgttgcctg gggtgttgca gacgctctgg
    10681 accgcgatcg gcagtgtgtc gcagaaacca taggcgccga cgtcctgggc gccggtcacg
    10741 aacggggcct ggtcaagcgg cgcgccgtca aggtacgaaa ccgggtatcc gtggaaagac
    10801 caggtctggc tgtacgcgtc ctcgtcgaaa taacacatgt aaatcctggt ccatgcacac
    10861 gcatcgaccg tggccgccgg catttcgccc tcaacattcg actggaacat caactgaccg
    10921 ttctgtgggg aacacgccct gccatcctgg gggcacttgt agaac
//
LOCUS       DAOPPA010000041        10707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7040_length_10707_cov_3.248310, whole genome shotgun sequence.
ACCESSION   DAOPPA010000041 DAOPPA010000000
VERSION     DAOPPA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_10707_cov_3.248310"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKH54_02265"
     CDS             <1..505
                     /locus_tag="PKH54_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98739.1"
                     /translation="SDEEDQSSGDLGFYIDFFRSIKGFYNQNMMHFNAIVGVDDGTLV
                     FQDGTKQPGCIGPTGSGAYEAKRYIEVANATNGKVGSICDPNYKEVLTEIGSVAFGLK
                     QQFFLSRIADPNSITVKVKGVQLADNDGWEYDAASNSIIFDVESPNMPQEGDEIEVHY
                     ETLCFEP"
     gene            572..2710
                     /locus_tag="PKH54_02270"
     CDS             572..2710
                     /locus_tag="PKH54_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98740.1"
                     /translation="MSIGRFPGRFMFVAVALAALAVGGFSGCKGGGAGVSPELYVTAT
                     GAVDSSMERVNNGENRDFREGGGTMVLVFGNMGDDILQISNIEIVEKNEDMLISQDVT
                     AFQYPIFVENGDYREVHETVVKLNITYVPDADRDLTPTRIRLTTNDPMYAGGIFEFTV
                     SPNEKTAEIKVTPNNYIFMGATSMNPATASFTVENVGDADLALASIRVEPASAYSITY
                     TSRPIHENMIIDPTVEGSAEGPIEVVVQYRPVTPPDLASLEIQWGSLIDTGVECSGTP
                     TCQNSDKLCPDKSKSCPYTCFNGRCNCTFSDQCRAYFCDDPQSPDCKWRCLTGVCRVP
                     ELTSVPLRGEAQAGSLEITHGDQADGCVNFQSVTVPGTSCTKALSLANPGPGSVLVTK
                     PTVQVSGSPETSPYTVQWFKLGAQKDGECGNVTGEEITENKYTLTPENSPITVAITYV
                     APADSGFNGEMKVLFANPYDGEQIVSLCGGDKKGELAIAPAPEKVRMTLYAAPGTTAK
                     KSFVVMNKGNLGLEIAGVSVEPANLEDPQAFSILESATGPFTLSEGEVRRFTVSFNGT
                     HDGGTTVNGFVNIDYVDPLAGEGGQVINQKINVAGFNSFDGVTLPVADAGQVSGPIKA
                     GDSVVLDGTASVPGSYPIPANSGYIWFVSKKPPASRVFLNSAAAQGQLTVVPDVGGDY
                     EFRLVVFSVEPDSGQAYFSHESTLSFSVMD"
     gene            2904..>10707
                     /locus_tag="PKH54_02275"
     CDS             2904..>10707
                     /locus_tag="PKH54_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98741.1"
                     /translation="MEVTDFLVELEGNPDDAVMFDRAVEEALAEGGVEGLESFLAQVM
                     EFLGDPAVAETFVKKADAFFRRERTSDKGRIVAWAAAVVAWKILNDLVRAEFFMRGVG
                     EDKLPDVDTWLEFYRVFYASRGNWLRLEQFMGEAVQKFGKPEIEMKRTLARTAAEANN
                     STKELSYWQEVAKLDPGDDEASSSLEKLYAQLQRWPSLAELYKARFTALPDDAVTEKV
                     FALKQMLEIYDQKMRAEPKVLSTYQMILDVDPANIEAMDSLLAKYEAAGRWPDYAKVL
                     DRKIESASDIRQKVGLMEKKAELMETRFANAMEAMKAYEAILEIDPERRDILNKLKDL
                     YEKRHDYENLIRIRRAEADSMPDGAEKAAQYADLAVMATERLRKFPVAIELWELVLIL
                     DESNDDALRALEGLYERTKELDKLAVVLQRRQAMTSNPAESIAILEKLAQLFGTKLED
                     ANGAMETWRKILDVDPNHERAKRELRTRYLGQRKWDELEWFLRRYGNPEELARTLEGQ
                     VNSIPDLEEKKGVLFKLAAIWRDELGQATRAVKNLEGVLAVAPNDARAAGDLIDLYRG
                     LSDWRKLPKVYEVAIAGAADFYQRQSLRVEAAEVQKVQLNNTKEAFFLYLAAFQEDML
                     NGELQEQLELLAGPSDNWDVYVAALEQAVNVMTETSIRIRTWLRIGEIYSENLGDIEA
                     GQAAFKRALDMDTGNREAITALEAIYRKLNRHEDLIRILQMRLSLVTDIEERKAVRFE
                     IATALYDCMGAVDEAVATWNDILAADPLEFTAYSQLGELLLKEKRYEELRGLLNRQID
                     AMFTLDQIDPVMMADLYSRVGLLTSGIDGNGSAAVDAYETALNYVPGHAATIEMLEDL
                     IGVEELRPRLIDLLKGPFEAAGRDADLADLIEMELQIKGDSIDTIDLLWKLEELYAGG
                     ASNEPKRFRVLSRIMMVTPDVTRAWDNLELVTASLGSWRSLVDLYSICVEMIEMPDAR
                     VSLNLRLARILWEELKSVDEARKAFHAVLALDDSNETALDALETIYEGLENHPELLKV
                     YRRRFEVSPYSGEKIAYAFKMAAELSDHLEDIEGAIEAIRKVLEIDPEYSAAWRQLDN
                     LYVRAERWLDLADVLGRRISLAEGDADRTWLRLRLAETLETKLEDLGGAVNVYQTILD
                     GDPANADTLVQLERLFDNPDVKVLVAPILLPAYRASGDDLKLVAVYDVLAEAADYVDT
                     RLMHYATIAEIYEINLKDLDRAFEYRSKAFSTAPERRELVDEVLRLGAARQKIDEAVH
                     VLAGKVFDIGDEEPRRETHRIIAKVCREARVDRELSKRHYSAVLGIDPTDMDAIDNLI
                     EMYVEDSETEKLVALIMVKADLVHGTAEKAKLLLWAGDLYAGSLSKDEEAISAFNGVL
                     DLDPRDMEAIVALEALYEKNEMWMELVDVLGRKADFTESHEKVAALKKKGLVQHDRME
                     NTVEAIETYLQVMAFSPGDVEGLRTLDRMYGAAEDWWNLYSTLESLHELVSGEERLTI
                     HFRMGKLLEREIGDATRAVQVYEALLSEFPDNKDAVEALEGMVRADLAAEDAFRILAP
                     TLSERQEWMRLYVVYEVITDREEDVARKVANLLTMGEIAEMRMEEPLRGFECFGKAFV
                     ADPLNRDARERIESLAASHDMWENVPGLMLEGALVIDGSPEALGLRLHAARILRDRLS
                     DLKASAAAFEKIIEDIPDNHEALEALDELYSTLEDWKELARILRAEFDASREVADKVR
                     FMLRFATVCEDRLNDSAAALDARQEVLFLVPNQSDAVAALRMMFNAGKHRAQVLDILE
                     PIYVDTSAWEDLTTVYEAALADVSEASSRRGIMVKLAEVWEKKRQELRPAMTWYGKAF
                     ELDATDESLLIQIEMLAGQSADWKLLLDILLAAAKPCEDEERKIYLWHKALETARDKL
                     SDLEKAEQIGKWVLEVSATDRTALAALDAMYESQQRWADLLGILNSEVAASEYDDEKI
                     GFLMRVGALQRERLGNLDGAVAAYKQIAQADEMHRPALTALSELHDQRGEWEELFNVL
                     SRLSDVAPESHVRSGLQRRMAVLAEEKLEQSETALGLWDEVSRADMSDVEALRNLQRL
                     YAAKGDWAAYVDSCERELPLATDDQSRTGELLRGIAVAAEKELGDTFQAQNAWRRILD
                     AAPGQMDALQALRRLYRESGDVDSLAKTLEAIYDTGTFQGAELKKLLEELAQLLTEEL
                     PRFEVAIKWWNKVVEIDGNDVAALKSLDRLYEEMGRFGECVGVIKRLVDLSSDNQEKA
                     EMLSRAASMQSDQMKDLAAAAETLQTVVGIVGNSMDVSERLGDLYVRLENWDALAETM
                     LARDEIIKDIDDRVNNLSEIARVYENRKGEKENAFLVYVKSADVAPTDENTFIELWRL
                     ARDLQNWGDYVDMVGSVVERMGDDLRLEHLVRFGEVLSKHTERKADAIGWYEKVLKDW
                     PENESALVALTDLYAEAGNHGKLVDVLEKRVDLSADYIEKVELQFRAGQVLENEVHDP
                     KRAVVAYAKILDFDDSHVQTLDALIRLYEQLKDWEKLVGALDRKAGTMPPDEIDIRFR
                     AGTVLEEKVGDAKRAIKAFEAVLEQNPAHSAAIDRLQSLYGGLEDWAGLAVAFTAATA
                     TARHR"
BASE COUNT         2262 a         3009 c         3382 g         2054 t
ORIGIN      
        1 gtcggacgag gaagaccaga gcagcggcga cctcggcttc tatatcgatt tcttcaggtc
       61 gatcaagggc ttctataacc agaacatgat gcacttcaat gcgatcgtcg gcgtcgatga
      121 cggcacgctg gtgttccagg acggcacgaa gcagccgggc tgtatcggcc cgaccggctc
      181 cggcgcatac gaggcaaagc gttacatcga agtcgcgaac gcgaccaacg gaaaggtcgg
      241 ctccatctgc gacccgaatt acaaggaggt tcttaccgaa atcggcagcg tcgcgttcgg
      301 cttgaagcag cagttcttcc tttcgcgaat cgccgacccg aactcgatta cggtaaaggt
      361 aaagggcgtc cagcttgccg acaacgatgg atgggaatac gacgcggcgt cgaattcgat
      421 catcttcgac gttgaaagcc cgaacatgcc gcaggaaggc gacgagattg aggttcacta
      481 cgagacgctc tgtttcgagc cgtagtcggg gattcatgat tgatgtgacg ggtggacggc
      541 gttccgcccc gacaaataaa ggagtttgtt tatgagtatc ggacggttcc cgggtcgatt
      601 catgtttgtc gcggttgctc tggctgcatt ggcggtcggc ggcttttccg ggtgcaaggg
      661 tggcggagcc ggggtctctc ccgagttgta cgtgacggcg accggtgccg ttgacagctc
      721 gatggagcgc gtgaacaacg gcgagaatcg tgatttccgt gaaggcggcg gcaccatggt
      781 gctggtcttc ggaaacatgg gcgacgatat ccttcagatt tccaatatcg agattgtcga
      841 gaagaacgaa gacatgctga taagccagga tgtcaccgcg tttcagtacc cgattttcgt
      901 ggaaaacggg gattatcgcg aagtccatga gaccgtggtc aagctgaaca tcacctacgt
      961 cccggacgct gaccgcgatc tgacgccgac caggatccgc ctgacgacga acgacccgat
     1021 gtacgccggc ggcattttcg agttcacggt ttccccgaat gaaaagacgg cggaaatcaa
     1081 ggtaaccccg aacaactaca tcttcatggg tgccacttcc atgaacccgg ccaccgcgtc
     1141 gttcacggtc gagaatgtcg gcgatgccga tcttgcgctt gcgtcgattc gtgtcgaacc
     1201 ggcctcggct tactcgatta cctatacttc gcgtccgatt cacgaaaata tgattatcga
     1261 tccgacggtc gaaggttccg ccgagggtcc gatcgaggtc gtcgtccagt accggccggt
     1321 gacccccccg gatctggcca gcctcgaaat ccagtggggt tcgctgatcg ataccggcgt
     1381 cgagtgctcg ggaacgccga cctgccagaa cagcgacaag ctctgcccgg acaagagcaa
     1441 gtcgtgtccc tacacatgct tcaacggtcg ttgcaactgc acgttctcgg atcaatgccg
     1501 cgcatatttc tgcgatgatc cgcagtcgcc cgattgcaag tggcgttgtc tgaccggcgt
     1561 gtgccgcgtc cccgaactga ccagcgtccc gctgcgcggc gaggcccagg cgggttcgct
     1621 tgaaatcacg cacggcgacc aggccgacgg ttgtgtcaat ttccagagcg tcaccgttcc
     1681 gggaacctca tgcacgaagg ctttgagcct tgccaatccc ggtccgggat ccgttctggt
     1741 cacgaagccg acggttcagg tttccggttc acccgaaacc agtccctata cagttcagtg
     1801 gttcaagctg ggcgcgcaga aggacggcga gtgcggtaat gtgaccggcg aggaaatcac
     1861 cgagaacaag tacacgctga cgccggaaaa cagtccgatt accgtcgcga tcacctacgt
     1921 ggcgccggcg gacagcgggt tcaacggaga aatgaaggtt ctgttcgcga atccgtacga
     1981 tggcgagcag atcgtcagcc tgtgcggtgg tgacaagaag ggcgaactgg ccatcgcccc
     2041 ggcccctgaa aaggtcagga tgacattgta tgccgcacct ggaacgactg ccaagaagtc
     2101 tttcgtcgtc atgaacaagg gcaatctggg cctcgagatt gcgggcgtca gcgtcgagcc
     2161 cgcgaatctg gaggatcccc aggcgttcag catccttgaa tccgcgaccg gtccgttcac
     2221 gctgtctgaa ggtgaagtca ggcgtttcac cgtttccttc aacggcaccc atgacggcgg
     2281 caccacggtc aacgggttcg tgaatatcga ttacgtcgat ccgctggcag gtgaaggcgg
     2341 tcaggtcatc aaccagaaga tcaacgtcgc cggcttcaat tcctttgacg gggtgacgct
     2401 gcccgtggcc gacgctggcc aggtttccgg cccgatcaag gccggcgatt cggtggttct
     2461 cgacggtacg gcaagcgtgc ccggttccta tccgattccg gctaactccg gctatatctg
     2521 gttcgtttcc aaaaagcccc cggccagccg ggtcttcctg aattcagcag cggcccaggg
     2581 ccagctcacg gtcgttcccg acgtcggtgg cgactacgag ttccgtctgg tggttttctc
     2641 ggtcgagccg gacagcggtc aggcctactt cagccacgaa tcgacactgt cattctccgt
     2701 catggattga cccaccaagg tgtgattccc gtctggttgg cagaaccgtt gctcgggaac
     2761 ccgactttcg cgcacgttca cgcgtaaaga aaatataaac atacatacct ttggcgcgcc
     2821 tgtttccctt gatgtaaacg tgcggctctg ataaagatat gcggacggca tttttcctgc
     2881 tccacgttga ttaggggtgt gtgatggaag taaccgattt cttggtggaa cttgagggca
     2941 acccggatga cgcggtcatg ttcgacaggg ctgttgaaga agctctggcc gaaggcggtg
     3001 tcgagggtct tgagtcattc cttgctcagg tgatggaatt tcttggcgat ccggccgtgg
     3061 ccgaaacttt cgtcaagaag gcggatgcct ttttccgccg cgaacgcacc agcgacaagg
     3121 gtcggatcgt ggcatgggct gccgccgtgg tggcctggaa gatcctgaat gacctggtcc
     3181 gggccgagtt cttcatgagg ggagtcggtg aagacaagct gcccgatgtc gatacgtggc
     3241 tggaatttta tcgcgtgttt tacgccagcc gtggcaactg gcttcgtctc gagcagttca
     3301 tgggcgaagc ggttcagaag ttcggcaagc ccgaaatcga gatgaagcgc accctggcca
     3361 gaaccgcggc cgaggccaac aattcgacaa aggagctttc ttactggcag gaagtcgcca
     3421 agcttgatcc gggcgacgac gaagccagca gcagccttga aaagctttac gcccagcttc
     3481 agcgctggcc gtcgcttgcc gagctttaca aggcccgttt caccgcactc cccgatgatg
     3541 ccgtcaccga aaaggtcttt gccctgaagc agatgctgga aatctatgac cagaagatgc
     3601 gggcggaacc gaaggttctt tccacgtatc agatgatcct ggacgtggat ccggccaaca
     3661 tcgaagcgat ggactccctg cttgccaagt acgaggcggc cggcaggtgg cccgactacg
     3721 ccaaggttct tgatcgcaag attgaatcgg cttctgacat tcgccagaag gtcggcctga
     3781 tggaaaagaa ggccgaactg atggagacca ggttcgccaa cgcgatggag gcgatgaagg
     3841 cttacgaggc catcctggaa atcgaccccg aacggcgcga tatccttaac aagttgaagg
     3901 atctttacga gaagcgtcat gactatgaaa acctgatccg cattcgcagg gccgaggccg
     3961 attcgatgcc cgatggtgca gaaaaggcgg cccagtatgc cgacctggcg gtcatggcaa
     4021 ccgagagact gcgcaagttc ccggtggcaa tcgagctgtg ggaactggtt ctgattcttg
     4081 acgagtcgaa tgacgatgcc ctgcgtgccc ttgaggggct ttacgaaaga accaaggaac
     4141 tggacaagct ggcggtcgtc ctgcagcggc gccaggccat gacttccaac ccggccgaga
     4201 gcatcgcgat cctcgagaag ctggcccagt tgttcggaac caagctggaa gatgccaacg
     4261 gcgccatgga gacctggcgg aagatcctgg atgtcgatcc gaatcacgaa cgcgccaagc
     4321 gcgaattgag gactcgttac ctcgggcagc gcaagtggga cgaactggaa tggttcctgc
     4381 gccgctacgg caatccggaa gaactggcgc gaacactcga aggccaggtc aacagcattc
     4441 cggatctgga agaaaagaag ggcgtcctgt tcaagctggc cgccatctgg cgcgatgaac
     4501 tgggccaggc gacccgcgcg gtcaagaacc tggaaggcgt cctggcggtt gcgccgaacg
     4561 atgcccgggc cgccggggat ctgatcgacc tttaccgcgg attgagcgac tggagaaagc
     4621 ttccaaaggt ctatgaggtg gcaatcgcgg gcgcggcgga cttctatcag cggcaaagcc
     4681 tgcgcgtcga ggcggccgag gttcagaagg ttcagctcaa caacacgaag gaagccttct
     4741 tcctttatct ggcggctttc caggaggaca tgctcaacgg cgagctccag gaacagcttg
     4801 aactgcttgc cggaccgtcc gacaactggg acgtctatgt ggccgcgctg gagcaggccg
     4861 tcaacgtgat gaccgagacc tcgatccgca tcaggacgtg gttgcgcatc ggtgaaattt
     4921 acagcgaaaa cctgggcgac atcgaggccg ggcaggccgc cttcaagcgc gcgctggaca
     4981 tggacaccgg caaccgcgag gcaatcacag cgctcgaggc catttaccgc aagctgaatc
     5041 gtcacgagga tctgatccgg atcctgcaga tgcggctgtc cctggtcacc gacatcgagg
     5101 agcgcaaggc cgtccgtttc gagatagcca cggctctgta cgattgcatg ggtgcggtcg
     5161 atgaagccgt cgccacctgg aacgacatcc tggcggccga tcctcttgaa ttcaccgcat
     5221 acagccagct cggggaactc cttctgaagg aaaagcgata cgaggaactt cgcggcctgc
     5281 tgaacaggca gatcgacgcg atgttcacgc ttgaccagat cgacccggtc atgatggccg
     5341 acctttacag tcgtgtcggt cttctgacga gtggtatcga cggcaacggc agcgcagcgg
     5401 tcgatgcata cgagacggcc ctgaactacg tccccggcca cgcggcgacg atcgagatgc
     5461 tggaagatct gatcggcgtc gaggaacttc gccccaggct gatcgatctc ctgaagggcc
     5521 cgttcgaggc cgccgggcgc gatgcggacc ttgccgatct gatcgagatg gaacttcaga
     5581 tcaagggcga cagcatcgac acgatcgatt tgctctggaa actggaagaa ctgtatgccg
     5641 gcggcgcgtc gaacgagccg aagcgtttcc gcgtgctttc aagaataatg atggtgacgc
     5701 cggacgtcac ccgtgcctgg gacaatcttg agttggttac cgcttccctt ggttcatggc
     5761 gcagcctggt cgatctgtac tccatctgcg tcgagatgat cgagatgccg gacgcccgcg
     5821 tctcccttaa cctgcgcctt gcgcgcatcc tctgggaaga actgaagagc gttgacgagg
     5881 cacgcaaggc gttccacgcc gtcctggcgc tggacgacag caacgaaacg gcgctggacg
     5941 ccctggaaac catttatgaa ggtcttgaaa accatccgga actgctgaag gtctatcgtc
     6001 ggcgcttcga ggtttcgccg tactccggtg aaaagattgc ctacgcattc aagatggcgg
     6061 ccgagctttc ggatcacctg gaagatatcg agggtgccat cgaggcgatt cgcaaggttc
     6121 tggaaatcga tccggaatac tctgccgcct ggagacagct cgataacctg tacgtaaggg
     6181 ccgagcgctg gctggatctg gccgacgtcc tgggtcgccg catttccctt gccgagggcg
     6241 atgccgaccg cacatggctg cgcctgaggc tggccgagac gctggaaacc aagctggaag
     6301 acctgggtgg cgcggtcaac gtctatcaga ccatcctgga tggggatccg gcgaatgcgg
     6361 acaccctggt gcagcttgaa aggttgtttg acaacccgga cgtcaaggtt cttgtcgccc
     6421 cgatactgct gccggcatac cgcgcaagcg gcgacgacct gaaactggtc gcggtctatg
     6481 acgtcctggc cgaagccgcc gattacgtcg ataccaggtt gatgcattac gcgacgattg
     6541 ccgagattta cgaaatcaac ctgaaggatc tggaccgcgc gtttgaatac cggtcgaagg
     6601 cattcagcac ggcccccgag cgtcgtgaac tggttgacga ggttctgcgc cttggcgctg
     6661 cccggcagaa gattgacgag gctgtgcacg tccttgccgg taaggttttt gatatcggtg
     6721 acgaggagcc gcgtcgcgag acccacagga ttatcgcgaa ggtttgtcgc gaggcccgcg
     6781 ttgacaggga actttcgaag cgtcactact cggcggtgct cggcatcgac ccgacggaca
     6841 tggacgcgat cgacaacctg atcgagatgt acgtcgagga ttccgagacc gaaaagctgg
     6901 tcgccctgat catggtcaag gcggatctgg ttcacggaac cgcggaaaag gcgaagctgc
     6961 ttctttgggc cggcgacctc tatgccggca gcctgagcaa ggacgaggaa gccatcagtg
     7021 cgttcaatgg cgtccttgac cttgatccgc gcgacatgga agcaatcgtc gccctggaag
     7081 ccctttacga aaagaacgag atgtggatgg aactggtgga cgtccttggc cgcaaggcgg
     7141 acttcaccga aagccacgaa aaggtcgccg ccctgaagaa gaagggcctt gtccagcacg
     7201 accggatgga aaacacggtc gaggcgatcg agacctatct tcaggtcatg gcgttcagcc
     7261 cgggcgacgt ggaagggctt cgcacgctgg atcgcatgta cggtgccgcc gaggactggt
     7321 ggaaccttta ttccaccctg gaaagcctgc acgaactggt gtccggcgaa gagcgcctga
     7381 ccatccactt caggatgggc aagttgcttg agcgcgaaat cggcgacgcg accagggccg
     7441 tccaggtcta tgaagccctg ctgtccgaat tccccgataa caaggatgcc gtcgaggccc
     7501 tggaaggtat ggttcgtgcc gacctggccg ccgaggatgc gttccggatc ctggcaccga
     7561 cactttccga acgccaggaa tggatgcgcc tgtacgttgt ttacgaggtc atcaccgacc
     7621 gcgaggaaga cgttgcccgc aaggttgcaa acctgctgac gatgggcgaa atcgcggaaa
     7681 tgcgcatgga agaacccctg cgcggcttcg agtgcttcgg aaaggcgttc gtggcggatc
     7741 ccctgaaccg cgacgcccgc gagcgcatcg agtcgctggc cgcttcccac gatatgtggg
     7801 aaaacgtgcc cggcctgatg cttgaaggcg ccctggtcat tgacggttcg cccgaggcgc
     7861 ttggacttcg gctgcatgcg gcgcgcattc tgcgcgatcg cctgtcggac ctgaaggctt
     7921 cggccgcggc gtttgaaaag atcatcgagg atattcccga caaccacgag gcgctcgagg
     7981 cccttgacga gctttacagc acgctcgagg actggaagga actggccagg atccttcgcg
     8041 ccgagttcga tgcgtcgcgt gaagtcgcgg acaaggttcg gttcatgctg cgcttcgcca
     8101 cggtgtgcga ggacaggctg aatgattccg ccgcggcgct ggatgcgcgg caggaagttc
     8161 tgttcctggt cccgaaccag tccgatgcgg tcgccgcgtt gcggatgatg tttaatgcgg
     8221 gcaagcacag ggcccaggtt ctggacatcc tggagccgat ttatgtcgat acttcggcct
     8281 gggaagacct gaccaccgtc tatgaggcgg cccttgcgga cgtctccgag gcgtcaagcc
     8341 gccgcggcat catggttaag ctggccgagg tgtgggaaaa gaagcgccag gaactgcgcc
     8401 cggcgatgac ctggtatggc aaggcgtttg agctggatgc gaccgatgag tccctgctga
     8461 tccagatcga gatgcttgcc ggccagtcgg cggactggaa gctgctgctg gacatcctgc
     8521 ttgcggccgc caagccgtgc gaggatgagg agcgcaagat ctacctgtgg cacaaggccc
     8581 tggaaaccgc gcgtgacaag ctttcggatc ttgagaaggc cgagcagatc ggcaagtggg
     8641 ttctggaagt ttccgccacc gacaggacgg ctctcgcggc cctggacgcc atgtacgagt
     8701 cccagcagcg ctgggccgac ctgcttggca tcctgaacag cgaggttgcc gcttccgaat
     8761 acgacgacga aaagattggc ttcctgatgc gggtcggcgc gttgcagcgc gaacggctgg
     8821 gcaacctgga tggcgcggtc gccgcgtaca agcagattgc acaggccgac gagatgcatc
     8881 gcccggcgct taccgcgctg tccgaactgc atgatcagag gggcgaatgg gaagaactgt
     8941 tcaacgtcct ttcaaggctt tcggacgtgg ccccggaatc gcacgtcaga tccggcctgc
     9001 agaggcgcat ggcagtcctt gccgaggaaa agcttgaaca gagcgagacc gcgcttggcc
     9061 tgtgggatga agtatcccgc gccgatatga gcgacgttga ggcgttgcgg aatcttcagc
     9121 gcctttacgc cgccaagggc gactgggcgg cctatgtcga ttcgtgcgaa cgcgaactgc
     9181 cgcttgcgac cgacgaccag tcaaggactg gcgaactgct tcgcgggatt gcggttgccg
     9241 ccgaaaagga acttggcgac acgttccagg cccagaatgc ctggaggcga atcctcgacg
     9301 cggcaccggg ccagatggat gccctgcagg cgcttcgcag gctttatcgt gagtcgggtg
     9361 atgtcgattc actggcgaag acactggaag ccatttatga caccggcaca ttccagggcg
     9421 cggaactgaa gaagctgctg gaagagcttg cgcagttgct gaccgaggaa ctgccgcgtt
     9481 tcgaagttgc catcaagtgg tggaacaagg ttgtcgaaat cgacggcaac gatgttgcgg
     9541 cgctgaagtc tctcgaccgg ctgtacgagg aaatgggccg gttcggcgag tgcgtcgggg
     9601 taatcaagcg gctggttgat ctgtcgtcgg acaaccagga aaaggccgag atgctgtcca
     9661 gggcggcttc gatgcagtcg gatcagatga aggatctggc tgccgcggcc gagacgctgc
     9721 agacggtcgt cggcattgtc ggcaacagca tggacgtcag cgagcggctt ggcgaccttt
     9781 acgtcaggct ggaaaactgg gatgcccttg cggagaccat gctggcgcgt gacgagatca
     9841 tcaaggatat cgatgatcgc gtgaacaacc tttccgaaat cgcccgggtt tatgaaaacc
     9901 gcaagggtga aaaggaaaac gccttcctgg tctatgtgaa gtccgccgac gtggcgccga
     9961 ccgacgaaaa tacgttcatc gaactgtggc ggctggcccg cgaccttcag aactggggcg
    10021 actatgtcga tatggtcggc agcgttgtcg aaaggatggg ggacgacctt cgccttgagc
    10081 acctggtcag gttcggtgag gtcctgtcga agcacaccga acggaaggcc gacgccatcg
    10141 gctggtacga aaaggttctt aaggactggc cggaaaacga gtccgccctg gttgccctga
    10201 cggatctgta cgccgaggcc gggaaccacg gcaagctggt cgatgtcctt gagaagaggg
    10261 tcgatctgag tgccgactac atcgaaaagg tcgaacttca gttcagggcc ggccaggttc
    10321 tggagaatga agtccacgac ccgaagcgcg cggtggtggc ttacgcgaag atccttgatt
    10381 tcgacgacag ccacgttcag accctggacg cactgattcg tctttacgag cagttgaagg
    10441 actgggagaa gctggttggc gccctggatc gcaaggccgg cacgatgccg ccggacgaga
    10501 tcgacatcag gttccgggcc ggtaccgtcc tcgaggaaaa ggtcggggac gccaagaggg
    10561 cgatcaaggc tttcgaggcg gttctcgagc agaacccggc gcattcggcc gcgatcgaca
    10621 ggttgcagtc cctttatggc ggactggaag actgggccgg cctggccgtg gccttcaccg
    10681 cggccaccgc caccgcccgc caccgcc
//
LOCUS       DAOPPA010000042        10669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7068_length_10669_cov_4.078387, whole genome shotgun sequence.
ACCESSION   DAOPPA010000042 DAOPPA010000000
VERSION     DAOPPA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_10669_cov_4.078387"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKH54_02280"
     CDS             <1..684
                     /locus_tag="PKH54_02280"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOC98742.1"
                     /translation="RRIDSAMALIGSVSVIVVSALLGDSEWLFPVVQTVFVAGMLGLI
                     VVLLTPHPIENAGHRAAMVVTAIVYVGVAGAICVRMIKVPDEVAMVGRWAVLTAGVIT
                     WLNDTMAYFGGKLFGRSKMYPAISPNKTWAGSISGMIGSIGGVFAIRAIFGIDYPWLP
                     LLGLGIVGGVLGQLGDLAESMFKRSAGVKDSGNLLPGHGGMLDRIDAFLFVAPITWLW
                     LFVWFPVGL"
     gene            complement(729..2423)
                     /locus_tag="PKH54_02285"
     CDS             complement(729..2423)
                     /locus_tag="PKH54_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98743.1"
                     /translation="MKLKLKILILLVCVAGAGCAASKREVVSPGGVVTITNQSGEDLE
                     VFVDGFRTAVVSAGTEHVIDRLISGKRVLTVTGVETRASASRTFDLKVAQGEGWRVTP
                     LSAASGGGDGPARGCRIDVVNSTMETVSVVVNDSPGTDIPAGVRVRLDGISPGTALVQ
                     ATGSESGLVRKAEIPVRVDVVPTFSIIDSSGAVRFTNRSGRAAFVSAPEIPVRQVENE
                     KTALFQDIPTGKIRFSALDIGRRILGEFDVDVVRGQVTDIVINQPAGTLNVVSDIAFP
                     VSIFADGVLLGTCPARGGALFTGLVPGNNNLKAVGDRDNVVASTWLEITPQTNLWLIS
                     ETSGTPLKAGTGAVEVENPSREKLEMYVDGTLAGVIDPGSKRVAVDLLPGDHVVAVVG
                     KTSRSLVTATVAVRDGARARWKVAMPVATLSVMNSSRERVNVYSDGRLLGQADPATTS
                     SFPITQGEHRIEVTGAVSMNGISRTMVFPAGTTTKLPFAGPMVTVVATNTFTDEVEIR
                     INDRLIGKLKPNERVTVSDVQPGTLRLAAESTKRPVSMTVMINLAAGDAFDWNIVP"
     gene            2555..4216
                     /locus_tag="PKH54_02290"
     CDS             2555..4216
                     /locus_tag="PKH54_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98744.1"
                     /translation="MEGRAHKTLVVETGILCNNRCVFCYQHGYRRIAGADMLVPRETI
                     QERMEWGIANGFDELSLTGGEPTIRKDFIDILASARRMGYQRVAVTTNGSRLADVDFF
                     RQAVSAGLDGMGVSIHGGTSPVHEAVTGRAGSFPAAIKAIRNGVRAAMALGEKRFRLN
                     TFTVLHSGIVDDLASFTSMLAAIGVRLMILQPPIAGKSNVTPVEWVGLDRTLDAVRGA
                     AIAAASGGYRLKPFNIPPCLLADVADGLDLTMYGRLQYRESDREDPSGHIMGQEVGFV
                     RFPACARCRKAAFCSGISVALLPDSDLLQAFRSIDVQDDSPIWLAGTELLSTDGIRQI
                     LCRLSTRHVILCTGGTHRAGTGLFHALQSGEAGGRHRLGLIYQARDPGSSDRIICDAG
                     NGDWLLETVRSAEACGYRGGITISGSAEPGFATFLMKARAILKGGGPGDDVHVFVAGP
                     GSAPGGSWQALEQIAEIVGSWDAADRFVLLTGGHGTDWKPVFLSVDGRNRTPMFQKIE
                     SIDVPSTAFDGRILSLLNWSVPAGTDRSREVPADFLVESLDVRPF"
     gene            complement(4220..5869)
                     /locus_tag="PKH54_02295"
     CDS             complement(4220..5869)
                     /locus_tag="PKH54_02295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOC98745.1"
                     /translation="MTMFNINARDDMLAHLSGKVWDVLVIGGGITGAGIARDSAMRGL
                     DTALVERDDFSIGTSSRSSKLVHGGLRYLKNREFGLVHESTTERNLLTKKIAPNLVRP
                     LPFLVPIWQTSKPGYGAMRAAMVMYDVLAELNNFRAHRALTVSELLKRAPALRRDGLR
                     GGFVYYDSISDDTRLTLELVIDAHRHGAVPVSRVEALETEFENGRVRGVTVRDTINDR
                     RMFVHARSVIVATGIWSSETAAALGIAPAARLDIRPTRGSHIVLPYDKFPIDSAVTML
                     APRDKRAMFVIPWNGMTVLGTTDTDYQGDLCNVFCSRDDALYLLEAAHHNFPGITAGL
                     DDIVGTWSGLRPLIASEGSESAVSREHSIVVDDRGVVTIAGGKLTTYRIMADQALKAA
                     APFFNKPLRRSTAGRVPLPCSAGLADAAARRRLSDEIVTTTGVSAAIADNLVVNHGVR
                     ARDVAALVVKNPGLAAPILAGHPFILAEVVQAIRCEMAVTLADVLLRRTPVFFLGRDD
                     DESATRAAANVMAAELGWDQARVDSEIAQVAEIRRRHLECL"
     gene            6006..6938
                     /locus_tag="PKH54_02300"
     CDS             6006..6938
                     /locus_tag="PKH54_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98746.1"
                     /translation="MKRRLELSSGAAFGLALTLLITLAGCGDTVVARQKAAAEAQKSQ
                     APGEVTAKITSGPANAPETAGNIQAQPSAEEPATEPEKPKSRFHPAAKDADYRYLDKV
                     WETDEEREVIEYLTRAATAEKGYRIVKKNPQKWLPLLRKSIKADHREVRIQTAVILGL
                     LKDKSHETFLSLKDCLLLDSDPDVRAIAAKAFVVLKNKEAVQVLIRSMNEDPYEAARA
                     NAAWALGSIGDHDAVEHLRKATRDDDTFVRLRSVSALLKIKSKSAIPELIDRLEDKSP
                     MVRERAHEALRTITGTDKGKDREPWARAFSRPVE"
     gene            7062..7955
                     /locus_tag="PKH54_02305"
     CDS             7062..7955
                     /locus_tag="PKH54_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC98747.1"
                     /translation="MDFAVMGCMVFFLVLSAAFSGRIPSGTEPAIRFATILVCYMAAV
                     RILDRSPSPFGLRMFIRAVFLYVAFPFAFMALGHVIEFINPFRAEAALMEIDRLMFFG
                     ANPNAVLQDFLNPFVVEGLQIVYALYYPLFMLGFIFLFRRRYSEFMAYLTGIACLFWL
                     QFIGYTLVPARSPYIIADLPEYAARIGLHTRVEGLWLTDLIRHSIHSVEGLKFDCFPS
                     GHTAGAIYVTLSMFAFSRRWGIAVAPWCAALVFSTVYLQYHYVIDLFAGAALGVGAFY
                     LGRALTRRYPLHIPADLGGAR"
     gene            7952..8989
                     /locus_tag="PKH54_02310"
     CDS             7952..8989
                     /locus_tag="PKH54_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98748.1"
                     /translation="MNRRILLCAISGLAGAMFFINGCDGCGCGSKPDPASAPAVSTGS
                     VGDTAAQPARTPTRRDVVVQLGNMIPSDATWVAVFEVERVRETLHKVARNAPKLVRSR
                     DHHDIRKRLIDLYGIDLDKTSGPCLLAGLGDSGRFLVCAAGSDISSPAESAIWSVGPY
                     SGPIVFRRSALISTGAVEGRLVSGDQNSVIQALTLFRASQPSLGMLFSRDRNVFADLI
                     ADDGWRDFALFFPGMDPGWCDKAICRGTAVFGGRDGLLVSSIASDPDTAQTLRAAVEK
                     GWRDKVLTSWRKAGARPDVPPEFFSDAEAAINENSVAVRSDRVLFKASGDVYDLLSVI
                     FIEDLEWILGI"
     gene            9018..9437
                     /gene="ndk"
                     /locus_tag="PKH54_02315"
     CDS             9018..9437
                     /gene="ndk"
                     /locus_tag="PKH54_02315"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOC98749.1"
                     /translation="MTIERTLAIIKPDAFENHHVGEIIQCIEKSGLTLAGMKMLRLTA
                     SQVERFYYVHNDKYFFPLLCEMMLSGPVVVMLIEGESAISRWRQMIGPTDPAECGPET
                     IRGAFGESIRHNAVHGSDCPDSAAFETAFFFSESELV"
     gene            complement(9450..>10669)
                     /gene="gltX"
                     /locus_tag="PKH54_02320"
     CDS             complement(9450..>10669)
                     /gene="gltX"
                     /locus_tag="PKH54_02320"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOC98750.1"
                     /translation="LGPYRQSERTEIYRKHVRDLIDLGFAYPCFCTPEELNAIRLRQR
                     DLKQNTGYDGTCRHIPKDEAIRRMDAGETCVIRLKVPEGKTVVHDRLRGDVEIENSQV
                     DDQILLKSDGFPTYHLANVVDDHLMGITHVIRAEEWISSTPKHLMLYEAFGWEPPTFV
                     HLPLLRNHDKSKISKRKNPVSLLYFKQIGILPEAMLNFLAMLGHSMPDEREKFTLTEF
                     VNEFSFDRISLGGPVFDLEKLIWLNGVHIREMDDDELARRIVGLMFTPEAMKPLIPLI
                     KERIRTLGDYPEHVSYFNAPQIDVPLIDIWKAGKGMTTAQIAKMLKETVEFLDGIREF
                     DARWLEEGLRTFCEQKEYQVKTLFMMLRLCITGRKATPPLFDVFVVLGKAAVGQRIRA
                     AADAIAAWKPPAA"
BASE COUNT         2047 a         3118 c         3225 g         2279 t
ORIGIN      
        1 agacgcattg acagtgcgat ggccttgatt ggaagcgtct cggtaatcgt cgtttcagcg
       61 ctgcttggcg attcggaatg gcttttcccc gtcgtccaga cggttttcgt tgccgggatg
      121 cttggattga ttgttgtgct tctgacaccg caccccatcg agaatgccgg acaccgggcc
      181 gccatggtcg tcacggcgat tgtttacgtc ggagtggccg gagccatctg cgtccggatg
      241 atcaaggtcc cggacgaggt cgcgatggtc gggcgctggg ccgtcctgac tgccggtgtc
      301 attacctggc tgaacgacac gatggcttac ttcgggggca agctgttcgg tcgcagcaag
      361 atgtacccgg cgatcagccc gaacaagacc tgggccggat cgatttccgg gatgatcgga
      421 agcatcggcg gcgttttcgc gattcgcgcc atcttcggga ttgattatcc gtggcttcct
      481 ctccttggcc ttgggattgt cggcggagtg ctcggtcagc ttggcgatct tgcggaatca
      541 atgttcaagc gatcggccgg cgtcaaggat tccgggaacc tgcttcccgg ccatggcggc
      601 atgcttgatc ggatcgacgc cttcctcttt gttgcaccga taacctggct gtggctgttc
      661 gtgtggttcc cggttggcct gtaaggcggg tgctcgggat tcagcgttcc ggactcagga
      721 cgagcgcatc agggcacgat gttccagtcg aaagcatccc cggccgccag atttatcatc
      781 acggtcatgc tgacaggtcg tttcgtcgat tcggccgcca ggcggagggt gccgggctga
      841 acgtcggaaa cggtgaccct ctcgtttggc ttcagtttgc caatcaatct gtcgttaatc
      901 ctgatttcga cctcatccgt gaaggtgttg gtcgcgacga ccgtgaccat cggcccggca
      961 aacggcaatt tcgtggtggt gccggccggg aacaccatgg tgcgcgaaat cccgttcatg
     1021 ctgacggcgc cggtcacttc gattcgatgc tcgccctggg taatcggaaa cgaggaagtg
     1081 gttgccggat cggcctgtcc cagcaggcgt ccatcggaat agacgttcac cctttccctg
     1141 gatgaattca tcaccgaaag cgtcgccacc ggcatcgcga ccttccaacg ggcacgcgcg
     1201 ccgtcgcgta ccgcaacagt ggcggtcacg agcgaacgcg atgtcttgcc aacgaccgct
     1261 acaacgtgat cgccgggcag aagatcgacg gcaacccttt ttgagccggg gtcgatgaca
     1321 ccggcaagcg ttccgtcaac gtacatctcc agcttttccc ttgaagggtt ttccacctcg
     1381 accgcgccgg tacccgcctt caatggcgtt ccgctggtct cggaaatcag ccagaggttg
     1441 gtctggggcg ttatctcaag ccacgttgac gcgacgacgt tgtcgcgatc gccgaccgcc
     1501 ttcaggttgt tgttgccggg aaccaggccg gtgaatagcg ccccaccgcg ggccgggcat
     1561 gtaccaagca gtacaccgtc cgcaaatatc gaaacgggga aggcaatgtc cgacacgacg
     1621 ttcagcgtcc cggcgggctg gttgatgacg atatcggtga cctggccgcg aacgacatcg
     1681 acatcgaatt cgccaagaat cctgcggcca atgtccagag ccgaaaagcg aatttttcca
     1741 gtcggaatgt cctgaaacag ggcggtcttt tcgttctcca cctgtctgac cggtatttcg
     1801 ggggcggaaa cgaacgcggc gcgaccgctt cgattcgtga agcgcaccgc gccggatgaa
     1861 tcaattatgg aaaacgtcgg cacgacatcg acacgaacag gaatttcggc cttgcgaacc
     1921 agcccgcttt cgctgccggt agcctggacc aacgcggttc ccggcgagat tccgtcaagc
     1981 cgcacgcgaa cgccggccgg gatatccgtc ccgggggaat cattgaccac gacagaaaca
     2041 gtctccatcg tcgagttgac gacatcgatt cggcatcccc gagcaggccc gtcaccgcca
     2101 cctgatgccg cggaaagcgg ggtgacccgc catccctcgc cctgggcgac cttcaaatca
     2161 aacgtcctgc tggccgacgc tcgggtctcg acgcccgtga cggtcagaac gcgctttccg
     2221 gaaatcaggc gatcgataac atgttcggtg ccggccgaaa caacggcggt tcgaaatcca
     2281 tcgacgaaca cctcgagatc ctcgccggac tggttggtaa tcgtgacgac cccgcctgga
     2341 gaaacgactt cacgtttgga tgccgcgcat cctgcaccag ccacgcaaac caataaaatc
     2401 agtatcttta atttcaactt cataagtgca attccttcgg taacgtcccg accgaccccg
     2461 gggccaacgc aataaccgcc cggacaaacc acggcaaggt ttcgttgccc gaataataca
     2521 ctttaacaaa gggtttttgg gctaaaatcg cgccatggaa ggtcgcgccc acaagacgct
     2581 ggtggttgag acaggcatcc tgtgtaacaa caggtgcgtt ttctgctatc agcacggtta
     2641 tcgccgcatc gcgggcgcgg acatgcttgt gccccgcgaa accatccagg aaaggatgga
     2701 atggggaatc gccaacggtt ttgacgagct ctcgctgacc ggtggcgagc cgaccattcg
     2761 aaaggacttc atcgatatcc tggcgtcggc gcgccggatg ggctaccagc gcgtcgccgt
     2821 caccacgaac ggaagccggc tggcggacgt cgattttttc cgccaggccg tttcggctgg
     2881 tcttgacgga atgggcgttt cgattcatgg cggcaccagt cccgttcacg aggccgtcac
     2941 cggacgcgcg ggatcttttc cggctgcaat caaggcaatc aggaacgggg taagggccgc
     3001 catggcgctt ggggagaagc gcttcaggct taacaccttc acggttctgc attccgggat
     3061 tgtcgatgat ctggcgtcgt tcacgtcgat gctggcggcg attggcgtcc ggctgatgat
     3121 ccttcagccg ccgattgccg ggaagtctaa cgtgacgccg gtggaatggg tggggctgga
     3181 caggacgctc gacgcggttc ggggggccgc gatcgccgct gcttcgggcg ggtatcgatt
     3241 gaagcctttc aacattccgc cgtgccttct tgccgacgtg gccgacgggc tggatctgac
     3301 gatgtacgga agacttcagt accgggaaag cgaccgcgag gatccgtccg ggcacatcat
     3361 ggggcaggag gtcggcttcg tgcgcttccc ggcttgcgcc cggtgccgga aggcggcgtt
     3421 ctgctccggg atcagtgtgg ctttgcttcc ggattccgac cttcttcagg cgtttcgatc
     3481 aattgacgtg caggacgatt cgccaatctg gctggccgga accgaattgc tttcgaccga
     3541 tggcatccgg caaattctat gtcggctttc aacgcgacac gtgattcttt gcaccggagg
     3601 gacgcacaga gcaggcaccg ggcttttcca cgcgcttcag agcggtgaag ccgggggccg
     3661 gcatcggctt ggcctgatct atcaggcaag ggatcctggt tcatcggatc ggattatctg
     3721 tgatgccgga aacggcgact ggctgcttga gaccgtccgg tccgcggaag cctgcggtta
     3781 ccggggtgga atcacaatca gcggttcagc cgaaccgggc tttgcgacat ttctgatgaa
     3841 ggcccgcgcg attctaaagg ggggcggccc aggcgacgac gtgcatgtat ttgttgccgg
     3901 ccccggaagc gcgccgggcg gctcctggca ggcgcttgaa cagattgccg aaattgtggg
     3961 cagttgggat gccgcagaca ggttcgtgct gttaaccggg gggcacggca ccgactggaa
     4021 accggttttc ctgtcagtcg atggacgaaa ccggacgcca atgtttcaga agatcgaaag
     4081 catcgacgtg ccttcgacgg cctttgacgg gcgaatcctt tcacttctca actggtcggt
     4141 gccggccgga accgaccgct cccgcgaagt gccggcggac tttcttgttg aatcccttga
     4201 tgtcaggccg ttctgaagac tacaggcatt ccaggtgccg ccggcggatt tcggcgacct
     4261 gggcgatttc cgaatcgaca cgcgcctgat cccagccgag ctcggccgcc atgacgttcg
     4321 ccgcagccct ggtggcggat tcatcgtcgt cgcgaccaag gaagaagacc ggggtacgtc
     4381 gaagaagcac gtcggcaagc gttacggcca tttcgcagcg gatggcctgg acgacttccg
     4441 cgagaatgaa cgggtgaccg gcaaggattg gtgcggcaag tcccggattt ttcacaacaa
     4501 gcgcagccac gtcgcgggca cgcacgccgt gattgacaac aaggttgtcg gcgatggccg
     4561 cactgactcc ggtcgtcgtc acaatctcat ccgaaaggcg gcgccgggcg gccgcgtcgg
     4621 caaggccggc ggagcagggc agggggacgc ggcccgccgt cgatcggcgc aacggcttgt
     4681 tgaagaaggg cgccgccgcc ttcagtgcct ggtcggccat gatccgatag gtcgtcagct
     4741 tgccgcccgc aatcgtcacc acgccgcgat cgtcaacgac gatgctgtgt tccctggaca
     4801 cggcggattc ggaaccttcc gacgcgatca gcgggcgcag ccccgaccag gttccgacga
     4861 tgtcgtcgag gccggcggta atccccggga agttatgatg agccgcttcc agcagataca
     4921 gagcgtcgtc ccttgagcag aacacattgc aaaggtcgcc ctggtagtcg gtgtcggttg
     4981 ttccgagcac cgtcatcccg ttccacggaa taacgaacat cgcccgtttg tcccttggcg
     5041 ccagcatcgt caccgccgaa tcgatcggga atttgtcgta cggaagaacg atatggcttc
     5101 ccctggtcgg gcggatatcc agccgggcag ccggggcgat gccaagtgcg gccgcggtct
     5161 ccgatgacca tatcccggtc gcgacaatca ccgaccgggc atgaacgaac atccgcctgt
     5221 cgttgatggt gtcgcgaacc gtgacgccgc gcacccggcc gttctcgaac tccgtttcaa
     5281 gcgcctcgac ccggcttacg ggaacggcgc cgtggcgatg cgcgtcaatc acgagttcaa
     5341 gggtcagtcg cgtgtcgtcg ctgatcgaat cgtaatagac gaatccgcca cgcaggccat
     5401 cccggcgaag cgccggcgcg cgcttgagca gttcggaaac ggtcagggcc cggtgagccc
     5461 ggaagttgtt caattcggca agcacgtcgt acatgaccat ggccgcccgc atcgcgccgt
     5521 agccaggctt cgacgtctgc cagatcggca cgaggaaggg aagtgggcgc acaaggtttg
     5581 gcgcgatctt cttcgtcaga aggttgcgtt cggtcgtgct ttcatgaaca agcccgaatt
     5641 cgcggttctt caggtagcga agcccgccgt gaaccagctt gctgctgcgc gaggacgttc
     5701 cgatcgaaaa atcgtccctt tcgacaagtg cggtgtcgag ccctcgcatt gccgagtccc
     5761 ttgcaatgcc ggcaccggtg attccaccac cgataacaag aacgtcccag accttgccgg
     5821 acaggtgcgc gagcatgtcg tcgcgagcgt tgatattgaa catagtcatt cgaaatcccc
     5881 tgattaagcc cgtttgaagg ggcgatagac cgaaaagctg tttttaatat agaatgcctc
     5941 gcgaagagta acaatggccg cgtgacgcgg accggatttt gccttgttca tccagggggc
     6001 ctttgatgaa aagaagactt gaattgagtt cgggtgccgc cttcgggctt gccctgacat
     6061 tgttgataac ccttgcgggg tgtggtgaca ccgtcgttgc caggcagaag gccgcggctg
     6121 aggcacaaaa gagtcaggcc cccggggaag tgactgcaaa gatcacctcc ggcccggcca
     6181 acgcgccgga aaccgccggg aacatccagg cgcagccttc agcggaagag ccggcgacag
     6241 agcccgagaa gccgaagtca cgcttccatc cggcggcgaa ggatgccgac taccgttacc
     6301 tggacaaagt ctgggaaacc gacgaggaac gcgaagtcat cgaatacctg acaagggccg
     6361 caaccgctga aaagggctac cgaattgtaa agaagaatcc gcagaagtgg cttccgttgc
     6421 ttcgaaagtc gattaaggcg gatcaccggg aggtcaggat ccagaccgcc gtcatcctgg
     6481 gtctgttgaa agacaagtcg cacgaaacgt tcctttcgct gaaggattgc ctgctgcttg
     6541 attccgaccc ggatgtgcgg gcaatcgcgg caaaggcctt cgtcgtgctg aagaacaagg
     6601 aggccgttca ggttctgatt cgttcaatga acgaagatcc ttacgaggcg gcacgcgcga
     6661 acgccgcctg ggcgctcggc tcgattggtg atcacgatgc ggtcgagcat cttcgcaagg
     6721 caacaaggga cgacgataca ttcgtcaggc tgcgttcggt cagcgctctg ctgaaaatca
     6781 agtcgaaatc ggcgattccc gaactgattg atcggctgga agacaaaagc ccgatggtac
     6841 gggaaagggc tcacgaggcg ctccggacga tcaccggcac ggacaaggga aaggatcggg
     6901 aaccatgggc cagggcgttc agtcggccgg tggagtgagg ggcgcgagcc aaccttccat
     6961 gatacgcgag ttgacataat atgtcttatc agacgttgct tatctggtga acaatgacaa
     7021 ggggaaccgc ttcaagtcat cctcgatatg gctttgagat catggacttc gcagtcatgg
     7081 gctgcatggt ttttttcctg gttctctcag cggccttttc gggtcggata ccctcgggaa
     7141 ccgaaccggc aattcgattt gcaaccattc ttgtctgcta catggcggcc gtccggattc
     7201 tcgatcgatc tccgtcaccg ttcggcttga gaatgttcat ccgcgcagtg tttctttacg
     7261 tcgcgtttcc attcgccttc atggcccttg ggcacgtgat tgagttcatc aatccgttcc
     7321 gggccgaggc cgccttgatg gaaattgacc gcctgatgtt tttcggcgcc aatcccaacg
     7381 ctgttcttca ggattttctg aatcctttcg tcgtcgaggg cctccagatc gtctatgcgc
     7441 tttactatcc gcttttcatg ctgggcttca ttttcctgtt tcggcggcgc tattccgaat
     7501 tcatggccta tctgactggt attgcctgcc ttttctggct ccagttcatc ggttatacgc
     7561 tcgttccggc gcggtcgccc tatatcatcg cggatcttcc ggaatacgcc gcccggattg
     7621 gcctgcacac gcgggtcgag gggctctggc ttaccgacct gatcaggcac tcaatccact
     7681 cggtcgaggg gctgaaattc gactgttttc caagtggcca cactgcgggc gccatttacg
     7741 tgacgctttc gatgttcgcc ttttccaggc gctggggcat tgctgtcgcg ccctggtgcg
     7801 ccgcgcttgt tttttcgaca gtttatctgc aatatcacta cgttatcgat ctttttgctg
     7861 gtgccgcgct tggtgttggg gctttttacc ttggacgcgc gcttacgcgc aggtatcctt
     7921 tacacattcc cgctgatttg gggggagccc gatgaaccgt cgtattttgc tttgtgcaat
     7981 ttctggcctt gctggcgcca tgttcttcat caacggttgc gacggctgcg gttgcggctc
     8041 caagccggat ccggcatcgg cgcccgcggt gtccaccggt tcggtcggtg atacggcggc
     8101 gcagccggcg cggactccga cccgcaggga cgtcgtggtc cagctgggca acatgattcc
     8161 gtccgacgcg acgtgggttg ccgtcttcga ggtcgagcgg gtgcgcgaaa cccttcacaa
     8221 ggtcgcccgc aacgccccca agctggttcg ttcccgcgac caccacgaca tccggaagcg
     8281 tttaattgac ctgtatggaa tcgacctgga caagacctcg ggcccgtgcc tgcttgccgg
     8341 gctgggcgac agcggccgtt tcctggtctg cgccgccggt tcggacatat cttcacctgc
     8401 cgaatccgcg atctggtcgg ttggaccgta ttccggacct attgtgttcc gccgaagcgc
     8461 ccttatttca acgggcgccg tcgagggacg cctggtctcg ggcgaccaga actcggtaat
     8521 ccaggcactg actctgtttc gcgcctctca acccagcctt gggatgctct tttccaggga
     8581 tcgcaacgtc tttgccgacc tgattgccga cgacggatgg cgggacttcg ccctcttctt
     8641 ccccggcatg gatccgggtt ggtgcgacaa ggcaatctgc cgtggaacgg ctgttttcgg
     8701 gggccgtgac ggccttctgg tatcctcgat tgccagcgat cccgacaccg cgcagacact
     8761 gcgggccgcc gttgaaaagg gctggcgtga caaggttctg acgtcttggc gcaaggccgg
     8821 cgcgcgaccg gatgtgcccc cggaattctt ttcggatgcc gaggcggcga taaacgaaaa
     8881 cagtgttgcc gtaaggtctg atagggtgct tttcaaggcc tcgggcgacg tttatgatct
     8941 gttaagcgtg attttcattg aagacctgga gtggattctg ggcatataat ggtgcttcaa
     9001 cctgttgggg gcctgatatg actattgaac gaaccctggc catcattaag cccgacgcct
     9061 ttgaaaacca tcatgtcggc gaaatcatcc agtgcatcga aaagagcggt cttacgcttg
     9121 ccggcatgaa gatgctgcgg ctgacggcat cccaggttga acgcttttat tacgttcaca
     9181 acgacaagta cttctttccg ctgctttgcg aaatgatgct atccggcccc gtggtcgtca
     9241 tgctgatcga gggcgaaagc gcaatcagca ggtggcgtca gatgatcggg ccgacagatc
     9301 cagccgaatg cgggcccgag acgattcgtg gcgcctttgg cgaaagcatc cgtcacaacg
     9361 cggttcacgg atcggactgc ccggactcgg ccgcatttga gaccgccttt ttcttttcgg
     9421 aatccgaact ggtgtaatct ggcggatttt caggcggctg gcggcttcca ggcggcgatg
     9481 gcatcggcgg cggccctgat tctctggccg acagcggcct ttccgagcac gacgaagaca
     9541 tcgaacagcg gcggggtcgc cttgcggccg gtgatgcaca ggcgcagcat catgaacagc
     9601 gtcttgacct ggtactcctt ctgctcgcaa aaggttctca gtccttcttc aagccaccgg
     9661 gcatcgaact cgcggattcc gtccaggaac tcgacggtct ccttgagcat cttggcgatc
     9721 tgagcggtcg tcatgccctt ccctgccttc cagatatcaa tcaggggcac atcgatttgg
     9781 ggcgcattga agtacgaaac atgttccgga taatcgccaa gtgtccggat tcgttccttg
     9841 atgagcggaa tcaaaggctt catcgcctcg ggcgtgaaca tcagccccac gatcctgcgc
     9901 gcaagttcgt cgtcatccat ctcgcggatg tgaacgccgt tcaaccagat cagcttctcc
     9961 aggtcgaaca ccggcccgcc caggctgatc cgatcaaaac tgaattcgtt gacgaactcg
    10021 gtcagggtga acttttcgcg ctcgtccggc atcgaatgcc cgagcattgc aaggaagttc
    10081 agcatcgctt cgggcaggat cccgatctgc ttgaaataaa gcagggaaac agggttcttg
    10141 cgcttggaaa tcttggactt gtcgtgattc cgcagcagcg gcagatgcac gaaggtcggc
    10201 ggctcccagc caaaggcctc gtaaagcatc aggtgcttcg gcgtgcttga aatccactcc
    10261 tcggcgcgta tgacgtgggt aatccccatc aggtggtcgt caaccacgtt cgccaggtgg
    10321 tacgttggaa agccgtcgga cttcagcaga atctggtcgt caacctggct gttctcgatc
    10381 tcgacatccc cccgaagcct gtcgtgaacg acggtctttc cttctggaac cttcagcctg
    10441 atgacacagg tctcgcccgc gtccatccga cggattgcct cgtccttcgg aatgtggcga
    10501 caagtgccgt catagccggt gttctgcttc aggtcgcgct ggcgaagcct gatcgcgttc
    10561 agttcttccg gcgtgcagaa acaggggtac gcgaaaccaa gatcaatcaa gtcgcgaacg
    10621 tgtttccggt agatttcggt ccgctcggac tgccggtatg gccccaagg
//
LOCUS       DAOPPA010000043        10613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7113_length_10613_cov_3.815401, whole genome shotgun sequence.
ACCESSION   DAOPPA010000043 DAOPPA010000000
VERSION     DAOPPA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7113_length_10613_cov_3.815401"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="PKH54_02325"
     CDS             complement(<1..1964)
                     /locus_tag="PKH54_02325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC98751.1"
                     /translation="MGSGFRLVAGVLFLILASACGGGAEVGPDSVTDAAADVRDSGSP
                     DDGTLADARDAQFPDVPVDVVQEDLQDAWAADDGVESDEDLPDSGEVPIDIGSDDSPP
                     SDVLGRVPGEVVYWRVSPEFRILGPDLIYTTSPSIVVLPNGDYLISFNLFGNSLEPSA
                     EVSGTTYIYRSSDKGATWTCLTPEPMMDMKRGSLFVHDGAVYLWGYTAAPGRIVIRRS
                     DDNGSTWTEPVDDQSGLLTADTYGGTPGNPVFHDGRVWMAVAGKRVMSAPQDSDLLLA
                     ASWSGPSLAADTNGGPLGDGLTITEAQIVASPAIGVSVLPKIDGLPFTVVISADGDAS
                     VRNPSEWDWASFPGGEKKFGAGYDPVSKRFWALSNPVPQVHANHPEQGPQLIRNTAAL
                     LSSRDLRQWDVRQIFLYSPNVDYEAFQYLNFDIDGDDMIIVSRTAFDITPLGLFKPPR
                     GHESNMITFHRIEDFRNAFPTHYLVVADGEVLRYERTGYADAPLGRFTLGSVFDDMML
                     DAVTGLADGGDGTVLVREERGRVLRFDLSGNFLGVATGSTAVFEPESIYIGQPPEGEC
                     TWIRDGDGAWADSGNWYYWGRPDYGMEDAVFGTVGTAPRKVEIDIEYRVRGLRFSGEH
                     GYELAGEGSLYVEAVTGKGQVAVTRGAHKIG"
     gene            complement(2050..2220)
                     /locus_tag="PKH54_02330"
     CDS             complement(2050..2220)
                     /locus_tag="PKH54_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98752.1"
                     /translation="MKSNSRARLHGIGGSTIEMVHGISGWTLMMTPSSTGIVYLRVRF
                     SISSVGFSTNAG"
     gene            complement(2207..3418)
                     /locus_tag="PKH54_02335"
     CDS             complement(2207..3418)
                     /locus_tag="PKH54_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98753.1"
                     /translation="MRSGFIFAVVVIAMFAGCGGDPEVTRNDLPYDPDIQRPDVSGDV
                     LTGDDIPIRDGEDQDVTGPQDVAGTDTNGGDTAGTDTPAVGDAIGQDTTVPQDAVQPQ
                     DTNEPGDNGTGDPDVVITPIPFACASGKTVVDGVNKSWTVAGKTREFTVKFPDNPDGK
                     PVSVIFVYHGFGDSVANFMKFFGPNPDADPDFPFVLVYPKSLALMPYGADKGIEWTIL
                     NSQSGDNNLDAKLFEEILGCLDDTVTSGISDVFVFGFSAGAIFGNLLHSRYPDLVRSV
                     MTMSGAWFNDDDTVKGVNTMGMATLGWDDLDNGGGTVFMTHGGTNDTYGSMGITIIDF
                     EKSAGFAVPHLTGHDRTVIDCPHTSGHTNHPGIGTSAVIRYFKDHRGGVISPWVSGGL
                     PAVYPEGCEIK"
     gene            3635..4537
                     /locus_tag="PKH54_02340"
     CDS             3635..4537
                     /locus_tag="PKH54_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOC98754.1"
                     /translation="MPENRVELEFSKMPNATGGIIRTLFAGRPAQAPENRLGISYRAT
                     WKGGRADPAVLARYRNLVGMSDDGTIPTMYIHALVMPLRLTMMTHGAFPMRLMGLVHF
                     TNRIEVTRPIRADETFDIVSTIEGIKVTEKGQAFDAVTELSVGGEVVWKEIATFLAPL
                     PPGKRAKDGKPAAPKPEEPDWGEPIATWNVPGNAGRVFSSIASDINPIHMSAFTAKLF
                     GFKRAIAHGMFSAGRCYAEILKTTPIESPVAIDVRFKRPLFIPGKVALHAIKDADDTR
                     FLLKVLPKGEPHVEGRISKLQSST"
     gene            4702..7350
                     /locus_tag="PKH54_02345"
     CDS             4702..7350
                     /locus_tag="PKH54_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOC98755.1"
                     /translation="MKKISSIIVASAFSILVVNGCGSSSDPIDDVIPDTTEPEKALLR
                     AIHLSPDAPEVDIFANEAETAVVTGLAFGEGTGYLELIPGNWNFDLAPAGSGIGASVL
                     DTGALALEEGKSYTAVAYDTLSALKVMALVDDYQELDAGNIRVRAIHTAAGVGQVDIW
                     NIPATGSPTALYTNVDFGVAGAYMDLPAGAYKLGFDVDNDATPDLVFRTPNLAAGAVI
                     NLFAVKSGDEVYLLAQLNDGTVEKIEAEEEVVVVDKTWLRAIHLSQDAPNVDIFAKDA
                     EPAVVTDLAFQEGTEYLELEAGTWKFDLAPAGMGLSATVLDTGDLNLEKDKRYTAVAF
                     NYVSYLEVMALEDNLEGLEAGKIRIRPIHVAPAVGQVDIWNIPAEGDPTALYTDVDFK
                     AVGAYADLPAGAYRLGFDVDNDAIPDVIFQTPELAAGTIINLFAVSQGSEVFLIAQLQ
                     DGTVARIDAEVMPKAMVRAIHLSPDAPAVDIFANEGEPAVLTGLAFPEGTPYLDVAPG
                     TYNFDISPEGTGLAGSVIDTGDLELEEGKYYTAVAFGEVANLQVLPLIDNYEGLDAGK
                     FRVRPIHAADGVGQVDIWNIPEEGDPAPLYTDVDYGVVGAYADLPAGSYKLGFDVNND
                     GVPDVSFQTPEIDAGAVINLFAVRDGSEVFLIAQLNDGSTAKIDAIHPPKARVRAIHL
                     SPDAPAVDIFANETEPAVVTGLDFQDSSGFLMVDPGTYNFDLSPADAGLAGSVLDTGD
                     LELQDGVSYTAVAYDQVSALKVMPLVEDFEGLAEGQIRVRPIHAAYMVGQVDIWNIPE
                     TGDPAPLYTDVDFGAVGAYVDLPAGAYKLGIDVDNNAVPDLVFQTPDLAAGSIINVFA
                     VQDGDGVFLLAQFQDSFVARINPLAP"
     gene            7659..9227
                     /locus_tag="PKH54_02350"
     CDS             7659..9227
                     /locus_tag="PKH54_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98756.1"
                     /translation="MLQKVSFVTLRQFLAATTLIAALGAVSCGTSEDPQDVTINIDIQ
                     VPDFGGCVPATCESLQAECGTPQDGCGNALSCGECEVGSCEDFKCRTICIPQCFGKCC
                     GDDGCGGACTDNCALNGQECDTETCTCGAACPTRTTCQDLGKNCGTWDDGCGGTQDCG
                     TCTTGECVDGACQEPPPEGLNDPCPGGDDDCSEAFPVCAPVGEGFCSKECTKDADCGS
                     STCCVMTGQKKHCMAPDNDFVCSDPSTQTGCPGGYNCMPVQNSACTFCYEVGEIPAGG
                     QCSYVNDCVKGYLCTKSTGEATSTCKKLCNAKAGTGCPGGQTCGGLTGVTDWGICRTG
                     PATCNPANNGNDCPEGQSCYGSSGCTSFTCGQHGSLGLGAECTYSDDCGKGMICGNGV
                     CMQMCGNGSCPEGFDCVDVSGCTGLKVCAGQGGTACTIGKNNEPCGANEMCYPLDADC
                     SQAGCYQAGTAGPNESCTNINDCQKGYLCANDGSGGTCLKACWSVADCSPPYHCEFMG
                     NGCGQTIGSCSNVM"
     gene            9380..10426
                     /locus_tag="PKH54_02355"
     CDS             9380..10426
                     /locus_tag="PKH54_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC98757.1"
                     /translation="MLGLDSLKITPDILRRISTIDEFKGAWMALGRLAPERLSSLRRV
                     ATIESVGSSTRIEGVKLTNAQIEGLLRGLQMHSFRSRDEQEVAGYAEAMELIFHSWAE
                     IGLTENHIKQLHGILLKYSDRDQRHRGQYKSIPNHVEAFDQEGRSIGVIFETATPFDT
                     PRMMFDLISDANALLSDNSHHPLLIIAAFIVRFLAIHPFQDGNGRLSRALTTLLLLRS
                     GYGYVPYSSLERVIEDNKDAYYMALRTAQVTIDRGEDGLPAWIEFFLTALIRQKQILE
                     SKLQAEQRLMALPELSVQILEIARNHGRVTLTDACSMTGANRNTIKHHIKALVQQGLL
                     SINGKARGTWYEPR"
BASE COUNT         2282 a         3290 c         3045 g         1996 t
ORIGIN      
        1 ccaatcttat gcgccccgcg cgtgacggcg acctggccct taccggtgac ggcctcgacg
       61 tacaggcttc cttcgccggc cagttcgtat ccgtgctcac cggaaaaccg caggccgcga
      121 accctgtatt caatatcgat ttcaaccttt cgcggagccg taccgacagt accgaaaaca
      181 gcatcctcca tcccgtaatc cggcctgccc cagtagtacc agttgccgga atcggcccag
      241 gctccgtccc cgtcccgtat ccaggtacat tcaccttcgg ggggctgacc gatgtaaatt
      301 gattcaggtt cgaatacggc cgtggaaccg gttgccacgc ccaggaaatt gccggacagg
      361 tcgaaccgca gaacccgccc ccgctcctcg cgaaccagga cggtgccgtc gccgccgtcc
      421 gccagtccgg taaccgcgtc gagcatcatg tcgtcaaaaa cactgcccag cgtgaaacgt
      481 cccagaggtg cgtcggcata ccctgtccgc tcgtatctca ggacctcgcc atccgccacc
      541 accaggtagt gagtcggaaa ggcatttctg aaatcctcga tccgatggaa ggtaatcatg
      601 ttcgattcat gaccgcgggg cggtttgaaa agtcccagcg gcgtgatgtc gaaagccgtt
      661 cgggaaacga tgatcatgtc atccccgtcg atgtcgaaat tcaggtactg gaaagcctcg
      721 tagtccacat tcggcgaata caggaatatc tgacggacgt cccattgacg aaggtccctg
      781 gacgacaaca gggccgccgt gttccggata agctggggcc cctgttccgg atgattcgca
      841 tgaacctgcg gaaccgggtt tgaaagtgcc cagaatctct ttgaaaccgg atcgtatcct
      901 gcaccgaatt tcttctcgcc tcccggaaac gacgcccagt cccattcgga cggattcctg
      961 actgaagcgt caccgtccgc actgatgacg actgtgaacg gaagtccgtc aatctttgga
     1021 agtacggaaa cgccgatcgc cggcgatgca acgatctggg cctcggttat cgtgagtccg
     1081 tcacccagcg gccccccgtt ggtgtcggcc gcgagcgagg gaccggacca ggacgccgca
     1141 agaagcagat cggaatcctg cggcgccgac atgacacgct tcccggcaac cgccatccag
     1201 acccgtccgt catgaaaaac cggattcccc ggcgtcccgc cgtaagtgtc cgccgtcagc
     1261 aggccgctct ggtcatccac cggttcggtc cacgtggaac cgttgtcgtc ggatctgcga
     1321 atcacgatcc tgccgggtgc ggccgtgtat ccccacagat aaacggcgcc atcgtgaacg
     1381 aaaaggctgc cccgcttcat gtccatcatc ggttcaggcg tcaggcaggt ccacgtcgca
     1441 cccttgtccg aagacctgta gatgtatgtc gtcccggaaa cctcggccga cggttcaagg
     1501 gaattcccga acagattgaa cgaaatcagg tagtccccgt tcggcaggac cacgatcgac
     1561 ggcgacgtcg tgtaaatcag gtcgggcccc agaatccgga attccggcga cacgcgccaa
     1621 tagacgacct cgccgggaac acgtccaagg acgtcggaag gcgggctgtc gtcgctgccg
     1681 atgtcgatcg ggacttcgcc cgaatccggc agatcctcgt ccgattccac cccgtcgtct
     1741 gcggcccagg cgtcctgaag atcttcctga acgacgtcga ccggaacgtc cggaaactgc
     1801 gcgtcacggg catcggccag cgtgccatcg tcaggggagc cagagtcgcg gacgtccgcc
     1861 gcggcgtcag tcaccgaatc cgggccaact tcggcaccac cgccacaggc agatgccaga
     1921 atcaggaaca aaacaccggc aaccagacga aatcctgaac ccacgactca ccccggaatc
     1981 agaggaatga cctgacgata accttacacc ccgtccaaga caataccgca cgaaacttta
     2041 cgactggtat cagccggcgt ttgtcgaaaa cccgacgctg ctgatcgaaa accgcaccct
     2101 cagatacacg atcccggtcg atgacggcgt catcataagg gtccacccac tgatgccgtg
     2161 aaccatttcg atggtcgatc cgccgatgcc gtgaagcctg gcccggctat ttgatttcac
     2221 agccttcggg atacaccgcc ggcaggccgc cggacaccca cggcgaaatt acgccgccgc
     2281 ggtgatcctt gaagtaccgt atgaccgcgg aagtgccaat ccccggatga ttcgtgtgac
     2341 cggacgtgtg cggacagtcg atgacggtgc ggtcatgccc ggtcaggtgc ggcaccgcga
     2401 agcctgccga cttttcaaaa tcgataatcg ttatgcccat gctgccgtaa gtatcatttg
     2461 ttccgccatg cgtcatgaag acggtgccgc cgccgttgtc caggtcatcc cagcccagcg
     2521 tcgccatgcc catcgtgttg acgcccttga ccgtatcgtc gtcgttgaac caggcgcccg
     2581 acatggtcat gaccgaccgg accaggtctg gataacggct gtgcagaaga ttcccgaaga
     2641 tcgcgccggc gctgaaacca aacacgaaaa catcactgat cccgcttgta accgtgtcat
     2701 ccaggcagcc cagaatttcc tcgaaaagct tcgcgtcaag gttgttatcg ccggattgac
     2761 tgttaagaat ggtccattcg atgcctttgt cggccccata aggcatcagt gccagggatt
     2821 tcggatatac aaggacgaaa ggaaaatccg gatccgcatc agggttcggg ccgaagaact
     2881 tcatgaagtt tgcgacgctg tccccgaatc cgtgatagac gaaaatgacc gacaccggct
     2941 tgccgtccgg attatcgggg aacttcaccg tgaattcccg ggtctttcca gcaacagtcc
     3001 aggacttgtt taccccatcc acaaccgtct tgccgctggc gcaggcaaaa ggtatcggag
     3061 taatcacgac atcgggatcg ccagtgccgt tatcccctgg ttcgttcgta tcctgcggct
     3121 ggaccgcgtc ctgagggacg gtagtatcct ggccaatggc atcgccgacg gcaggggtgt
     3181 cagtaccggc cgtgtcgccg ccgttcgtgt cggtgccagc gacatcctgg ggtcccgtga
     3241 cgtcctggtc ttcgccatcc cggatcggga tatcgtcgcc cgtcaacacg tcaccggata
     3301 cgtccggtcg ctgtatatca gggtcatacg gcagatcgtt gcgggtaacc tccgggtcac
     3361 cgccacaccc ggcgaacatt gcgataacaa ccactgcgaa tatgaatcct gatctcatgg
     3421 cgcccccctt tttccgacaa acccccggcc gcaattttcc gcgtacttcc ggtgaattat
     3481 gcgccgcgtt atggtcgcgt caataagctt tgaaaaaaat ccgatgcagc ccccgacaag
     3541 ccggtcttgc cgtcccgatt gactgacaga cactgttgac gccggcagcc ggtatgatga
     3601 tacttggcgc caggcttccc acaggggtaa tgcgatgccg gaaaacaggg ttgaacttga
     3661 attctcgaag atgccgaacg ccacgggtgg aatcattcgc acgcttttcg cgggacgccc
     3721 cgcgcaggca ccggaaaacc gtcttggcat cagctacagg gcgacgtgga aaggcggcag
     3781 ggccgacccg gccgtgctgg caaggtaccg caacctggtc ggcatgagcg acgacggcac
     3841 gatcccgacg atgtacattc acgctctggt catgcctctg cgcctgacca tgatgaccca
     3901 cggcgccttc ccgatgcgcc ttatggggct tgtgcacttc acgaaccgca tcgaggtcac
     3961 caggcccatt cgtgccgacg agactttcga catcgtcagc acgattgaag gcatcaaggt
     4021 caccgaaaag ggccaggcat tcgacgccgt taccgaactc agcgtcggcg gcgaagtggt
     4081 atggaaggaa atcgcgacgt tcctggcacc cctgcccccg ggcaagcggg caaaggacgg
     4141 aaagccggcg gcgccaaagc cggaggaacc ggactggggc gaaccgatag ccacgtggaa
     4201 tgttcctggt aacgccggcc gggtcttttc cagcatcgcc agcgacatca acccgattca
     4261 catgagcgcc ttcacggcga agctgttcgg gttcaagcgg gcaatcgcgc acgggatgtt
     4321 cagcgccggc cgctgctatg ccgagatcct gaagacaacc ccgattgaat cacccgtcgc
     4381 gatcgacgtc cgcttcaagc gcccgctgtt cattccgggc aaagtggcgc tgcacgcaat
     4441 caaggacgcg gacgacacca ggttcctgct gaaggttctg ccgaagggcg aaccgcacgt
     4501 cgaaggccga ataagcaaac ttcaatcatc aacctgatcc gccggccgga actgccctgc
     4561 acggccctcg atattgaccc cctgttattt cttgcaacac tggtcttgac acggtctgtc
     4621 aggatgtcga tgttttattc ggacaagaaa tgtcctgttt gaaacgtcaa catttcatta
     4681 aagaaatatg gaggctccca aatgaagaaa atttcatcaa taatcgttgc gtctgcgttt
     4741 tcgattcttg tggttaacgg ttgcggctca agttcggacc ccattgatga tgtgatcccg
     4801 gatacgaccg agccggaaaa ggcgcttctg cgcgccatac atctttcacc cgacgcgccc
     4861 gaggtcgaca tttttgccaa cgaagccgag actgccgtcg tcaccggact ggccttcggg
     4921 gaaggcaccg gataccttga actgattccc ggaaactgga atttcgacct ggcgccggcc
     4981 ggttccggaa ttggcgcttc cgtgctcgac accggcgcgc ttgcgctgga ggaaggcaag
     5041 tcctatacgg ccgttgcgta tgacacgctt tccgcgctga aggtcatggc cctggttgac
     5101 gattaccagg aactcgacgc ggggaacatc cgcgtaaggg cgattcatac cgccgccggt
     5161 gtgggccagg tcgatatctg gaacatcccg gcaacgggtt cgccgaccgc actttacacc
     5221 aacgtcgatt tcggggttgc cggcgcctat atggatctgc cggcgggcgc ctacaagctc
     5281 ggctttgacg ttgacaacga cgcaaccccg gatctggtct tccggacccc caatctggcg
     5341 gccggagccg taatcaacct gttcgcggtc aagagcggtg acgaagtgta cctgctggcc
     5401 cagctcaacg acggcacggt cgaaaagatc gaggcagagg aagaggtggt cgtggtcgat
     5461 aagacctggc tgcgtgcaat ccatctgtcc caggatgcgc cgaacgtcga tatctttgca
     5521 aaggatgcgg aacccgcggt cgtgaccgac ctggcattcc aggagggcac cgaatacctg
     5581 gaacttgaag ccggcacatg gaagttcgat ctggctccgg ccggcatggg actttcggcg
     5641 accgtcctgg acaccggcga cctgaacctt gaaaaggaca agcggtacac cgcggtggcg
     5701 ttcaactacg tttcctacct tgaggtgatg gcccttgaag acaatcttga gggcctggaa
     5761 gccggcaaga tccgtatccg cccgattcac gtcgcgccgg ccgtcggcca ggtcgatatc
     5821 tggaacatcc cggccgaagg cgatcccacc gcgctttaca ccgatgtcga tttcaaggcc
     5881 gtcggcgcat acgccgatct gcccgccggc gcgtacagac ttgggttcga cgtcgataac
     5941 gacgctatcc cggacgtgat cttccagacc ccggagcttg ctgcgggcac gattatcaac
     6001 ctgttcgcgg taagccaggg ctccgaagtc ttcctgattg cacagcttca ggatggaacg
     6061 gtggcgcgca tcgacgccga ggtcatgccg aaggccatgg tcagggcgat tcacctgtcc
     6121 cccgatgccc ccgcagttga catcttcgcg aatgaaggcg agccggccgt gctgaccgga
     6181 ctggcgttcc cggaaggaac cccatacctg gacgtcgcgc cgggaaccta caatttcgac
     6241 atctccccgg aaggcacggg gctggccgga tccgtaatcg acacaggcga ccttgaactc
     6301 gaggaaggca agtactacac cgccgtcgct ttcggcgagg tggccaatct tcaggttctg
     6361 ccactgatcg acaactacga aggcctggac gccggcaagt tcagggttcg tccgatccat
     6421 gcggccgacg gcgtcggcca ggtcgatatc tggaacatcc cggaagaagg cgaccccgct
     6481 cctctgtaca ccgatgtcga ttacggcgtg gtgggcgcgt acgcggatct tcctgccggg
     6541 tcttacaagc tggggttcga cgtcaataac gacggggttc ccgatgtcag cttccagacc
     6601 cctgaaatcg atgctggcgc cgtcatcaac ctgttcgcgg ttcgtgacgg ttcggaagta
     6661 ttcctgatcg cccagctcaa cgacggcagc accgccaaga tcgacgcaat ccatccgccc
     6721 aaggccaggg ttcgcgccat tcacctttcg cccgacgccc cggcggtcga tatcttcgcg
     6781 aatgaaaccg agccggccgt ggttacgggc ctggacttcc aggacagttc gggattcctg
     6841 atggttgatc ccggcacata caatttcgac ctgtccccgg ccgatgccgg tctggccgga
     6901 tcggtgcttg ataccggcga cctggaactg caggacggag tgtcctacac ggccgtcgcg
     6961 tacgaccagg tcagcgccct gaaggtgatg ccgctggtcg aggattttga aggactcgcc
     7021 gagggccaga tcagggtccg cccgatccac gcggcgtaca tggtcggcca ggtcgatatc
     7081 tggaacattc ccgaaaccgg cgacccggcc ccgctttaca ccgatgtcga tttcggcgcg
     7141 gtgggcgcat atgtcgatct cccggccggc gcttacaaac tgggtatcga cgttgacaac
     7201 aacgcggtgc cggatctggt cttccagacc cctgacctgg ccgctggaag catcatcaac
     7261 gtgttcgcgg tccaggacgg cgacggcgtg ttcctgctgg cccagttcca ggacagcttc
     7321 gttgcccgca tcaacccgct tgccccgtga accctgaccc aacctgacaa tcccgaccca
     7381 acctgacaat cctgaaccaa cctgacaatc ccgacccatc ctgacagcac cgtctccatc
     7441 aattttcccg taccttgcgt ggcaggattt catccggacg gttcccttcc cccggcgcct
     7501 cgcgtggact gattgcatcc acaaggttcc cttcccccgg cgcctcgtgt ggactgattg
     7561 catccgcacg gttcccttcc cgccagatct gatctagcat caccaaatcc gaacaggcag
     7621 gctttcggaa gaacatggat ttcttacagg aataaaaaat gcttcagaaa gtgtcttttg
     7681 tcacattgcg ccaattcctt gccgccacga cattgattgc cgctttgggg gcggtatcgt
     7741 gtggaacgtc cgaggatcca caggatgtga ccatcaacat cgacatccag gttccggact
     7801 ttggcggatg tgttccggcc acatgcgaat cgctgcaggc cgagtgcggc acgccacagg
     7861 acggctgcgg aaacgcgctg agctgcggtg aatgtgaagt cggatcctgc gaggatttca
     7921 aatgccgcac gatctgcatc ccccagtgct tcggcaaatg ctgtggcgac gacggatgcg
     7981 gcggcgcctg caccgacaac tgcgctctga acggccagga atgcgacacg gaaacgtgca
     8041 catgcggcgc cgcatgcccg acacgcacca cgtgccagga tctgggcaag aactgcggga
     8101 cctgggatga cggctgcggc ggcacgcagg actgcgggac atgcaccacc ggcgaatgtg
     8161 ttgacggcgc gtgccaggaa ccgccgcccg aaggactgaa cgacccctgc ccgggcggcg
     8221 acgacgactg cagtgaagcg tttccggtct gcgcaccggt tggcgaaggc ttctgcagca
     8281 aggaatgcac aaaggacgcg gattgcggtt catcgacgtg ctgcgtgatg accggccaga
     8341 agaagcactg catggccccg gacaacgatt tcgtctgcag cgacccgtca acccagaccg
     8401 gctgccccgg cggatacaac tgcatgccgg tccagaacag cgcgtgcacc ttctgctacg
     8461 aagtgggcga aatccctgca ggcggccagt gcagctatgt caacgactgc gtgaaaggtt
     8521 acctgtgcac gaaatcgacg ggtgaggcga catcgacctg caagaaactg tgcaacgcca
     8581 aggcaggaac cggctgcccc gggggccaga cgtgcggcgg actgaccggg gtcaccgact
     8641 ggggcatctg caggaccggc ccggcgacgt gcaatccggc aaacaacggc aacgactgcc
     8701 cggaagggca aagctgttat ggaagcagcg gctgcacctc gttcacctgc ggccagcacg
     8761 gcagccttgg cctgggcgcg gaatgcacgt attcggacga ctgcggcaag ggcatgatct
     8821 gcggaaacgg cgtctgcatg cagatgtgcg gaaacggaag ctgtccggaa ggcttcgact
     8881 gcgtcgatgt aagcggctgc accggcctga aggtatgcgc cggccagggt ggcaccgcgt
     8941 gcacgatcgg caagaacaac gaaccgtgcg gcgcgaacga aatgtgctat ccgctggacg
     9001 cggactgcag ccaggcggga tgttaccagg ccggaaccgc cggaccgaac gaatcctgca
     9061 ccaacatcaa cgactgccag aaaggctacc tgtgcgcgaa cgacggcagc ggcggcacct
     9121 gcctgaaggc gtgctggtcg gtcgcggact gctccccgcc gtatcactgc gaattcatgg
     9181 gtaacggctg cggccagacc atcggttcgt gcagcaacgt gatgtaacgt attcaggaac
     9241 cggttcgtat cctgcaaagc gacctgaatt ggcctcgata cgagtgctgc gccgaaaaca
     9301 tccaataaat ggcaatatcg acgataatcg gataatatcg gatcaactat tggataaaag
     9361 cacgttctgg agaaaataaa tgctcggact cgacagcctg aagatcacgc ctgacatatt
     9421 gcgacgcatc tccacgatcg acgaattcaa gggggcgtgg atggcacttg gccgactcgc
     9481 tcctgaacgt ctttcatccc ttcggcgggt ggccacgatt gaatctgtcg ggtcatcgac
     9541 cagaatcgag ggggttaagc ttacgaacgc ccagatcgaa ggccttcttc gtggcttgca
     9601 gatgcattcc ttccgatcca gggacgaaca ggaagtcgcc ggatacgccg aagccatgga
     9661 gttgatattc cattcctggg cggagatcgg cctgaccgaa aaccacatca agcaactgca
     9721 cggaatcctg ttgaagtact cggacaggga tcagagacat cgcgggcagt acaagtcgat
     9781 ccccaaccac gttgaagcat ttgatcagga aggaaggagc atcggcgtca tcttcgaaac
     9841 ggcaaccccg ttcgacacac cccggatgat gttcgacctg atatcggatg ccaacgcact
     9901 gctttccgac aattcacacc atcccctgct gataatcgcc gccttcatcg tacgcttcct
     9961 ggcgattcat ccgtttcagg acggaaatgg aaggttgtcg cgcgctttga ccacccttct
    10021 gctgttacgg tccggatacg gatacgtgcc atacagctcg ctggaacgcg ttatcgagga
    10081 caacaaggat gcctactaca tggctcttcg taccgcgcag gtaacaatcg accgtggcga
    10141 ggatggactt ccggcgtgga ttgaattctt cctgacggcc cttatccggc agaagcagat
    10201 tcttgaatcg aagctgcagg ccgaacagcg gcttatggcc ctgccggaac tgtcagtgca
    10261 aatccttgaa atcgccagga atcatggccg ggtgacgctg accgacgcat gctcgatgac
    10321 aggcgcgaac cgcaatacga tcaagcacca tatcaaagcc ctggtgcagc agggactcct
    10381 ttccatcaat ggcaaagccc gcggcacatg gtacgaacca cgctgacatt gtacgggcgg
    10441 attacatccg cccgccgcga accagcacgg tgccttcagt cacgcccccg cccccgccct
    10501 tgccaaaacc cctgataacc cttatattcc aaccctgctc gcgcaggttc tttgaaaagg
    10561 gggcgttccg gtttcgacgg ggatgcaatg ggaaaaggag cacgtccggg gtt
//
LOCUS       DAOPPA010000044        10593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7129_length_10593_cov_4.281458, whole genome shotgun sequence.
ACCESSION   DAOPPA010000044 DAOPPA010000000
VERSION     DAOPPA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_10593_cov_4.281458"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKH54_02360"
     CDS             <1..254
                     /locus_tag="PKH54_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98758.1"
                     /translation="RHAPRADAEERNRFQRYFASASFPAGRPAREERRIQRNKAIAMV
                     VADVDDDGCPDVVTADRKGRTISTLHNRTLACPGQEVRP"
     gene            251..3265
                     /locus_tag="PKH54_02365"
     CDS             251..3265
                     /locus_tag="PKH54_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOC98759.1"
                     /translation="MRKNAIQVPVLVLVTMVAALSSAGCQDTTFPFDLGGDIPADTGP
                     DSGDVEVWPDFGDVEPGTDFGGVDAGELPVEVDQLPPEISFESPTPEAGAAVNGQFEL
                     AFRVTDRGTGVRRIRVVMQDYEWKWKSPGGGGVEEYATADAGNLVIPVSGLRSGLYTI
                     EITAWDAVESHRSSLLRTVSVTRGVEFAAEAVSVPTPVDAGIGGSGIRIGPATENRWG
                     IVTSRGVFVREPEGVRTVSDGFDCDAVLWGVGDFNSDGTDDVVAYCRDKDAWKIAFLF
                     QDVDGRFSEVPAVEGERKLIDLAAGDLNGDGFADIAFAEVEETPLSLVKVSLSMTAED
                     GSLVGWGPVVEYIGAASPNAVEIGRFADNGRNAVLVSSELNGLFTVFPVDFAGNLLMG
                     ENSYFGELPFSAAGAADMCGGDGVPDTLVACNNCPKAPTMYVGRLDEVSGRVELIEGR
                     ISGLKPTGIAAGDVDGNGVGDVLVLCSGANMVVFFRGGDSCLEFGFDGDGIGMASGGA
                     THIWISDWDNDGYGDLFGLTSEGISVTYFRPDPDGGHFDASYQARFELVPLDVATGHF
                     VKPLESSGADFLDAAILTGYEVNILASGPRTGQPINTVTLIPSDPLAGSVRSQNGRIS
                     KEQGVFLGGRTYSGVASGMFGPPIFDANSGLRTDLHGLVMAGSNLAGPADPSSSLAVS
                     FQAESHEIGWFTLNPFFAGDRPAVLATGEFDLADPDGMTDVAFVWHEDKTEFQEARYY
                     LRPMLGDGLGGFTVHTPEGLGIQSLLIQENRLPGAMYAFALRRTLAQAEAGAAVPTDL
                     IVANRGTSDFTVFLGIGDGRFLAKYDGSLDFAVGQPPLDVAAGYLEGAFGSGAIDAGN
                     ELPDVVSLTRDGNSSVLVVSYALKPDELQARGLEVGFEVPHAMIIYQPGGPVTLNPVP
                     HPVALDIADVNNDGLVDILVLDFVRSSVLVFENHGKRNFADPVEFFTGTQPVAIRAAD
                     VDADGCVDILTADQSGKTMTVLRNRLCGARMD"
     gene            complement(3287..4954)
                     /locus_tag="PKH54_02370"
     CDS             complement(3287..4954)
                     /locus_tag="PKH54_02370"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOC98760.1"
                     /translation="MKTDVEIAQGAVTRPIAGIAEDIGIGPDELEQYGRGKAKVSLAA
                     FRRLRDRPDGKLVLVTAINPTAGGEGKTTMSVGLADAFRKIGIKSAVALREPSLGPCF
                     GMKGGATGGGYSQVVPMEDINLHFTGDFHAVTTAHNLLAAIIDNHIQQGNELDIDVRR
                     ILWRRVMDMNDRQLRNIVCGLGGKADGVPRESGFDITVASELMAILCLAGDIDDLKAR
                     IGRMIVAYTRSGRAVTASEFKVTGAMALLFKDAIKPNLVQTLEGTPAFIHGGPFANIA
                     HGCNSVMATRLAMKTAEVVVTEAGFGADLGAEKFMDIKCRYAGIHPDAVVIVASVRAL
                     KLHGGMPKNRLAEVDMRALEAGTANLEKHIENVAMFGVPAVVCINAFPTDTDEEIAFV
                     SERCAALGAESAVSRAWALGGEGSVDLAKKVWKAAGTPADFRFLYDVDSPVPDKITTI
                     ARKMYGADGVEFSDSARKTIRELEEAGLDKVPICMAKTQYSLSDDPARIGRPTGFTIN
                     VREIRPSIGAGFIVALTGTIMTMPGLPKRPAAEGMDISNDGTITGLS"
     gene            complement(5127..7292)
                     /locus_tag="PKH54_02375"
     CDS             complement(5127..7292)
                     /locus_tag="PKH54_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98761.1"
                     /translation="MAAVILATTASAYALEMVPDVPCCGLLGTINAALRSSRPSSWSW
                     ALMPVALLPLPLLVWLGAGFIRDQERLGPSRSEIIALLASACFSTIVIFTDVFSSLSP
                     WIDPDDVGELARVMSLSNGEYFPIQGDYIHMWRLYQGPIATMWFALFGVFFKNHLPFA
                     LAPLISVFMTQMTGAAFLRRTFGRNAAVIFILLVPFHMAFMMSSSSHVMFGLPLGIMM
                     VWILADAGRRNTDRHIPLYLLCAGIAVQLYAVNIAWIFTLPIIVTAFRRPSVRIRRWI
                     AGVLLFLLPQVFTLFMLVADLQAGKSISGNRLGGPPFALLIGSAVVALLSGIVLIGIR
                     FILGRKSLGQRMAAKPAIMIPSIFILHWGVVAAAAAFDLIEGTSFTFALASAYLAGAL
                     LVDLHARRLLAAFEPIRSRFIPAVVALSAIGYLAAWCSHAEGFRPPLSEPFRTDTTIA
                     PRLMNFLKDAGLDDRQAYRENAHHLHQVTISVPLYPMFFLPRADGTVDDEEPLEVVFL
                     PRRDSGDPAGDPFPDDPHAQMFSHNGLDVLAYPSRLSYAGQVATTVNCYGIASDFRPP
                     WPWQPRPLMGDKMTSARAMLGIPLSDANDPSRLGYGVVETGPDKPIPSRIRLKTPLKP
                     GSSRVLAVLVSTECESYVTIDQNPAELMVTLDYPDFFEDGDGYRVDLYEIDENGGLIT
                     VKVQVDDCSLQMLDLLDPPMDWQSMMQVLLEHGFDVRAF"
     gene            7526..>10593
                     /locus_tag="PKH54_02380"
     CDS             7526..>10593
                     /locus_tag="PKH54_02380"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOC98762.1"
                     /translation="MRSSLSLLRFPLLITFCAVFAGCGGGGPDTDDVDEPLDVLGDSS
                     GDNGGSCVDSCPEEGATDCSQGGVRTCADLDDDGCVEWSLPEACGQGLVCSEGKCVSG
                     CVDQDCTAIGAKMCSTSNNVIECGFFGEEACLAWGNSVPCDDGLVCARGFCASECVSE
                     CTTIGAKRCRMNSVVVCGDYNTDGCLEWGGEQDCGDQFCTNGVCKAECSDECTVINSR
                     QCELGGYVICNEYDGDGCLEWGTVLYCGQGQSCSNGFCSSECVPGCTVEGARKCQMNS
                     VVECGDFDNDGCLEWGSPTPCDEGLVCAGGNCANQCMNDCTVAKARQCDESGAVVTCD
                     DYNQDGCLEWGSPKQCDIGLVCSNGNCALSCSNQCQVDGARQCLAGSTTKFQVCGDYN
                     DDSCLEWGTAQDCEGSLVCSNGECAQTCSNGCDNADKLECVGDGGWRQCRDYNDDGCL
                     EWGTTVFCESWEECEAGKCESKEAPVKILINEIMIDTNGSPDLDSFVELWGPAGTSLS
                     GWSLIGVNGANGDEYQKIDLVGTLGSDGLFVVAHPDSKAAIHDMGDQFSSDVDYQNGY
                     DSIQIRYGETVVDAIAYDTFPPDKFFAGEGTAVPKPATDHSLARDLSHTDTDNNADDF
                     IELATPTPGAANQDVNQPPIAEVLCPATAKLGESATFDASGSDDPDGSIAEFKFDFGD
                     DSPAVSGLDSVVNHTFGAADTFTVTVTVTDNRGDKDTATCQIVVGDDNAPTVDFIKPA
                     NDTQVTQGSVVTVLVNPQAVPGRNIVRVELWADGVNTGMSDDSAPYEFTYVVPEGYPN
                     NTSLQLQAKAVDNLGSSGFGSVRLQVKNDVPIASFTAVVSGDKQITVDASSSRDTETA
                     TADLQVRWDFTNDGTWDTDWSAEKVVVKDYPADGQYSIKMEVRDAIGQTSSMVRDVTL
                     TSIQYVGGTVTTTTWTGTIVITGDVTVPVNETLTVAAGTSVQFVYIDQNGDQVGDYDI
                     TVNGTLIVNGTQQLPVLFTSYGTEHKHASAWNRILIQGAGSQIRNAAFEYA"
BASE COUNT         2074 a         3040 c         3384 g         2095 t
ORIGIN      
        1 gccgccatgc gccgcgggcc gacgcggagg agcggaaccg gtttcagcgc tacttcgcca
       61 gcgccagctt ccccgccggg cgtccggcac gcgaggagcg ccggatccag cgcaacaagg
      121 cgatcgcgat ggtcgtggcc gacgtcgatg acgacggatg ccctgatgtt gtgacggccg
      181 accgcaaggg ccgcacgatt tccacgctgc ataacaggac actggcctgc ccggggcagg
      241 aggtgcggcc atgaggaaga acgccattca ggtgccggtt cttgtacttg tgaccatggt
      301 ggcggccctt tcttcagcgg gttgccagga tacgacgttc ccgttcgact tgggcgggga
      361 catcccggcc gacacggggc ccgactcggg cgatgtcgaa gtctggcctg acttcggcga
      421 tgtcgaaccc gggacggact ttggcggggt tgatgcgggc gaacttccgg tcgaggtgga
      481 tcagttgccc ccggagatat cttttgaatc accgacgcca gaggccgggg ccgcagtaaa
      541 cggacagttc gaactggcat tcagggtgac tgaccgcggc accggggtcc ggcgcatcag
      601 ggtcgtgatg caggattatg agtggaaatg gaaaagtccg ggtggcggtg gcgtcgaaga
      661 gtatgccacg gccgacgccg gcaacctggt gatcccggtt tcgggtctca gatccggcct
      721 gtacaccatt gagatcacag cctgggacgc ggtcgaatcg catcggtcat cgttgcttcg
      781 gaccgtgtcc gtgacccgtg gcgtcgagtt cgcggccgag gccgtcagcg tgccgacgcc
      841 ggtcgatgcg ggtatcggcg gttccggcat caggatcggc ccggcgacgg aaaaccgctg
      901 gggaatcgtg acctcccgcg gggtcttcgt gcgcgagccc gaaggcgtca ggacagtttc
      961 ggatggcttt gattgcgatg cggtcctttg gggcgtgggc gacttcaatt ccgacggaac
     1021 cgacgacgtg gtcgcatact gtcgtgacaa ggatgcctgg aagatagcgt tccttttcca
     1081 ggacgtcgac ggccgatttt ccgaggtgcc ggcagtcgag ggcgaaagaa aactgattga
     1141 cctggccgcg ggcgacctga acggcgacgg gtttgccgat attgccttcg ccgaggtcga
     1201 ggaaacgccg ctgtccctgg tcaaggtgtc gttgtcgatg acggcggaag acggttcgct
     1261 ggtcggctgg gggcctgtcg ttgaatacat cggcgcagca agtccgaacg ccgtcgagat
     1321 tggccggttt gccgacaacg gacgtaacgc ggtgcttgtt tcgtccgagc tgaacggcct
     1381 tttcacggta tttccggtgg atttcgcggg gaacctgctg atgggcgaaa acagctattt
     1441 cggggaactt ccgttctcgg cggctggcgc ggccgatatg tgcggcggcg acggtgtgcc
     1501 tgacacgctt gtcgcctgca acaactgccc gaaggccccc acgatgtacg tcggaaggct
     1561 ggatgaagtt tcaggtcgcg tggaattgat cgaggggcgg atatccggcc tgaagccgac
     1621 cggaatcgcg gccggggacg tcgatggcaa tggcgtcggc gacgtgctgg tgctgtgttc
     1681 gggcgcaaac atggtcgtct tcttccgtgg gggcgacagt tgcctggagt tcgggttcga
     1741 cggcgacggg atcggcatgg cgtcgggcgg ggcgacgcac atctggattt cggactggga
     1801 taacgacggg tacggggatc ttttcggtct gacgtcggaa ggcatatcgg tgacgtattt
     1861 ccggcccgat ccggacggcg gtcatttcga cgcgtcctac caggcccgct tcgagctggt
     1921 tccgctggac gtggcgaccg ggcacttcgt caagccgctg gaatcttcgg gcgccgactt
     1981 cctggatgcg gcgatcctga ccgggtacga agtcaacatt ctggcctccg ggccccggac
     2041 gggacagccc atcaacacgg tcactttgat tccatccgat ccgcttgccg gatcggttcg
     2101 ttcacaaaat gggcggattt cgaaggaaca aggggtcttc cttggcggtc gcacatacag
     2161 cggggtcgct tctggaatgt tcgggccgcc gatcttcgac gccaattcgg gattgcgcac
     2221 ggatctgcac ggtctggtaa tggccggttc caatcttgcc ggtccagcag acccgagttc
     2281 atccctggcg gtcagctttc aggcggaaag ccatgagatc gggtggttca ccctgaatcc
     2341 gttctttgcg ggagatcgtc cggcggtgct cgcgacaggc gaatttgacc tggccgaccc
     2401 cgatggcatg acggatgtcg catttgtctg gcatgaagat aaaaccgagt ttcaggaagc
     2461 cagatattac ctgaggccga tgcttggcga cggcctgggc ggcttcaccg tccacacccc
     2521 tgaggggctt gggatccaat cgctgctcat ccaggaaaac cgtcttccgg gcgccatgta
     2581 cgcgtttgca ttgcgccgca cgcttgccca ggccgaagcc ggggccgccg tccccaccga
     2641 tctgattgta gcaaacaggg gaaccagcga tttcacggtc tttctgggaa tcggcgacgg
     2701 gcgtttcctg gccaagtacg acggaagcct ggatttcgcc gtcggtcagc caccgctgga
     2761 cgtggcggcc gggtatctgg agggtgcgtt cggttctggc gccatcgacg ccgggaacga
     2821 actgccggac gtggtttcgc ttacacgcga cggcaactcg tcggtgcttg tcgtgtcgta
     2881 cgccctcaag ccggatgaac ttcaggcgcg cggccttgag gtcggtttcg aggttccgca
     2941 cgccatgatc atctatcaac ctggcggccc cgtcaccttg aacccggtcc cgcatccggt
     3001 cgcgctggat atcgccgacg tcaacaacga cggccttgtc gatatcctgg tgctggactt
     3061 cgtccgttcg tcggtcctgg tcttcgagaa ccatggaaag cgcaacttcg ccgacccggt
     3121 agaattcttc acgggtaccc agccggtggc catcagggct gccgatgtcg atgccgacgg
     3181 ctgcgtggac atcctgacgg ccgaccagag cggcaagaca atgacagtgc taagaaaccg
     3241 gctttgcggc gcccgcatgg attgaaaccg acaccgataa gccgaatcac gacaatccgg
     3301 ttatcgttcc gtcgttcgaa atgtccatgc cttccgcggc cggccgcttc ggcaggcccg
     3361 gcatcgtcat gatggttccg gtgagcgcca cgatgaagcc ggcgccgatg gatggccgga
     3421 tctcgcggac gttgattgtg aatccggtcg gacgcccaat cctggccggg tcgtcggaaa
     3481 gcgagtactg ggtcttggcc atgcagattg gcaccttgtc cagaccggct tcctccagtt
     3541 cgcggatggt ctttcttgcc gaatccgaaa attcgactcc gtccgcccca tacatcttgc
     3601 gtgcgattgt cgtgatcttg tccggaaccg ggctgtccac gtcgtaaagg aacctgaaat
     3661 cggccggcgt tcccgcggcc ttccagactt ttttggccag atcgacgctt ccttccccgc
     3721 ccagggccca cgcgcgcgac acggccgatt cggcccccag cgcggcacat ctttcggaaa
     3781 cgaatgcgat ttcttcgtct gtgtcggtcg ggaacgcgtt gatgcagacg acggccggaa
     3841 ccccgaacat ggcaacgttt tcgatgtgct tttccaggtt tgccgtgccg gcttccagtg
     3901 cgcgcatatc gacttcggca agccggtttt tcggcatgcc gccgtgaagc ttcagtgccc
     3961 tgacggacgc cacgatcacc accgcgtccg gatggatccc ggcgtagcgg catttgatgt
     4021 ccatgaactt ctcggcgcca aggtccgccc cgaacccggc ttccgtgacg acgacttcgg
     4081 cggtcttcat cgccagcctg gtcgccatca cgctgttgca gccgtgggcg atgttggcaa
     4141 acgggccgcc atgtatgaat gccggcgttc cctccagcgt ctgaaccagg ttcggcttga
     4201 ttgcgtcctt gaaaagaagc gccatcgccc cggtaacctt gaattcggaa gccgtcaccg
     4261 cccttccgga acgcgtgtac gcgacaatca ttctgccgat gcgtgccttc aggtcatcga
     4321 tgtcgccggc aaggcaaaga atcgccatca gctccgacgc caccgtgata tcgaatccgc
     4381 tttcgcgcgg gacgccgtcg gcctttccgc ccagcccgca gacgatgttg cgaagctggc
     4441 ggtcgttcat gtccatcacg cgccgccaca atatccgtcg aacgtcgatg tcgagttcgt
     4501 tcccctgctg gatgtggttg tcgattatgg cagccagcag gttgtgggcc gtggtgaccg
     4561 cgtggaagtc cccggtgaaa tgaaggttga tgtcttccat cgggacgacc tgggaatatc
     4621 cgccgcccgt ggcgccgccc ttcatcccga agcaagggcc aagggaaggt tcgcgcagtg
     4681 cgaccgccga tttgatgccg attttgcgga atgcgtcggc aagtccgacg ctcatcgtcg
     4741 tcttgccttc gcctccggcc gtcgggttga ttgcggtcac aagaaccagc ttgccgtccg
     4801 gccggtcgcg caggcgccgg aaggccgcaa gcgatacctt ggccttgccg cgcccatatt
     4861 gttccagctc gtccggcccg ataccgatat cttcggcaat gccggcaatc ggccgcgtta
     4921 cggccccctg ggcgatttcg acatcggttt tcatcgtggt ttcccctttc aacttggttc
     4981 aacagccgtg gattcgcgga tctgccgcga ccggcaggga cacaatcgta tcagccggtg
     5041 gcggcacagg acaacaaccg caatggttac gggattattt ccagcatgtt gcgtcggcgc
     5101 gtcgatggat tgaaccggga cagccgtcag aaggccctca cgtcaaaccc gtgttcaagc
     5161 agaacctgca tcatcgactg ccagtccatc gggggatcca gcaggtccag catttgaagg
     5221 gaacagtcat ctacctggac tttcacggtg attaatccgc cgttttcgtc gatttcataa
     5281 agatcgaccc gatagccgtc gccgtcctca aagaagtcgg ggtagtcgag ggtgaccatg
     5341 agttcggcag gattctggtc gatcgtcaca tacgattcgc attccgttga aacaagaacc
     5401 gccagcaccc ggctggagcc cggcttgagt ggggtcttga gtctgattcg ggatgggatc
     5461 ggtttgtcag ggcccgtttc gaccacgccg tatccgagtc tggacgggtc gtttgcgtcg
     5521 gaaaggggaa ttcccagcat ggcccttgcc gaggtcatct tgtcgcccat caggggcctt
     5581 ggctgccagg gccagggtgg ccgaaagtcc gatgcgattc cgtaacagtt gactgtcgtc
     5641 gccacttgcc cggcgtatga aaggcgtgac ggataggcaa gcacgtcgag cccgttgtgc
     5701 gaaaacatct gggcgtgcgg atcatccggg aacggatccc cggccggatc gccggaatct
     5761 cttcttggca ggaatacgac ttcaagtggc tcttcgtcgt ccacggtgcc gtccgccctg
     5821 ggcaggaaga acatcgggta caggggaacg gaaatcgtga cctggtgaag gtgatgggcg
     5881 ttttcacggt atgcctgacg atcgtccagc ccggcatcct taaggaagtt catcagcctg
     5941 ggcgcgatgg tcgtatcggt tctgaaaggt tctgaaagcg gtggtcgaaa tccttcagcg
     6001 tgactgcacc atgcggccag atatccgatc gccgacaatg cgacaaccgc cgggatgaat
     6061 cgggacctga ttggctcgaa tgccgcaaga aggcggcgcg cgtgcaggtc aaccagcagg
     6121 gcgcccgcaa ggtatgcgga agcaagcgcg aaggtgaaac tggtgccttc gatcagatcg
     6181 aaggccgcgg cggcggcgac gacaccccaa tggagaatga aaatcgacgg aatcatgatt
     6241 gcgggcttgg cggccatgcg ttgtcccaga gattttcgcc cgaggatgaa cctgattccg
     6301 attagcacga ttcccgataa aagggcgacg acagccgatc cgatcagcag ggcaaacggc
     6361 ggacccccga gcctgttgcc ggaaatcgat ttacccgctt gcagatccgc caccagcatg
     6421 aacagcgtga aaacctgggg cagcaggaat agcagaacgc cggcaatcca tcttcgaatg
     6481 cgtacggatg gccgccggaa tgcggtcacg ataatgggaa gggtaaagat ccatgcgata
     6541 ttgacggcgt aaagctgaac ggcgatgccg gcgcacagca ggtacagggg aatgtgccgg
     6601 tcggtattgc gcctgcccgc gtcggccagg atccagacca tcatgatgcc cagcggcaat
     6661 ccaaacatca cgtgggacga cgacgacatc atgaaggcca tgtggaacgg gacaagcagg
     6721 atgaagatga cggcggcgtt cctgccgaat gtgcgtcgaa ggaacgccgc gccggtcatc
     6781 tgtgtcatga acaccgaaat cagcggagcc agcgcgaaag gcagatggtt tttgaagaag
     6841 acgccaaaca gcgcaaacca catcgtggcg atgggcccct ggtaaagtcg ccacatgtgg
     6901 atgtagtcgc cctggattgg gaagtattcg ccgtttgaca ggctcattac cctggccagt
     6961 tccccgacat cgtcgggatc gatccagggt gaaaggcttg aaaacacgtc ggtgaaaatc
     7021 acgatggtgg aaaaacaggc ggacgcaagc agcgcgatga tctcggatct ggacgggccc
     7081 agcctttcct gatccctgat gaaccccgcg cccagccaga caagcagcgg aagcggcagc
     7141 agcgccaccg gcatcagcgc ccagctccag gaagatgggc gcgatgatcg cagggcggcg
     7201 ttgattgttc cgagcagacc gcagcatgga acgtcaggaa ccatttccaa cgcatatgca
     7261 gaagccgtgg ttgcaagaat taccgcagcc attgtcgtga tcttcagaat cgcctgagat
     7321 gtgacgggca cgaattccgg catgcggggt ctttcaaagt gatgtgcatt cattgcgaca
     7381 aacgatatca ccgcccggcc ccatgtcaaa gagttgtggt gaatcgaacc tgctttgacc
     7441 tgtccggcaa ttgaagatgg tctggtaaag tggtaattac tgcggcgcct tttcaacact
     7501 ttcgtgattc atccggaggt catcgatgcg ctcaagtctt tcgcttcttc gttttccctt
     7561 gttgattacg ttttgtgccg tgttcgctgg gtgtggcggt ggcgggcccg ataccgatga
     7621 tgttgatgag cctttggatg tcctgggcga ttcgagtggc gacaacggcg gatcctgcgt
     7681 ggattcatgt ccggaagagg gtgcgaccga ctgttcgcag ggcggcgtaa gaacatgtgc
     7741 ggatcttgat gatgacggat gtgttgaatg gtcgctgccg gaagcgtgcg ggcaggggct
     7801 ggtgtgcagc gaaggcaagt gcgtcagcgg atgtgtggat caggactgca ccgccatcgg
     7861 tgcgaagatg tgctcaacct ccaacaacgt gatcgagtgc ggattcttcg gtgaagaggc
     7921 ttgccttgcc tggggcaatt ccgttccctg cgacgatgga ttggtctgcg cgcgcggatt
     7981 ctgcgcgtcg gaatgcgtca gcgaatgcac cacgattggg gcgaagcgct gccgcatgaa
     8041 cagcgtggtg gtctgcggcg actacaacac cgacggctgc ctggaatggg gcggcgaaca
     8101 ggattgcggc gaccagttct gcaccaacgg cgtctgcaag gccgaatgct cggacgagtg
     8161 tacggtgatc aacagccgtc agtgcgagct tgggggatac gtcatctgca acgagtacga
     8221 cggcgacggc tgtcttgaat ggggaacggt tctttactgc ggccagggcc agtcgtgcag
     8281 caacggtttc tgctcgtccg agtgcgtgcc gggctgcacg gtcgaaggcg cccgcaagtg
     8341 ccagatgaat tcggtcgtcg agtgcggcga ttttgataac gacggctgcc tggagtgggg
     8401 ttccccgact ccgtgcgacg agggtctcgt gtgcgcgggc ggcaactgcg cgaaccagtg
     8461 catgaacgat tgcacggtcg ccaaggcccg ccagtgcgat gaatccggcg cggtcgtgac
     8521 ctgcgacgac tacaaccagg acggatgcct ggaatggggc tccccgaagc agtgcgacat
     8581 cggtctggtg tgcagcaacg gcaactgcgc cctttcctgt tccaaccagt gccaggtcga
     8641 tggtgcaagg cagtgtcttg ccggctcgac aacgaagttc caggtctgcg gcgactacaa
     8701 cgacgacagt tgcctggagt ggggaaccgc ccaggattgt gaaggcagtc tggtctgcag
     8761 caatggggaa tgtgcccaga cttgctcgaa cggctgcgac aatgcggaca agctcgaatg
     8821 cgtgggcgac gggggatggc gccagtgccg ggattacaac gacgacggct gtcttgaatg
     8881 gggaacgacc gtattctgcg aatcgtggga agagtgcgag gccggcaagt gcgagtcgaa
     8941 ggaagccccg gtcaagatcc tgattaacga aataatgatc gacaccaatg gcagcccgga
     9001 cctcgactcg ttcgtcgaac tgtgggggcc ggccgggact tcgctgtccg gctggagcct
     9061 tatcggtgtc aacggagcca acggtgacga gtaccagaag atcgacctgg tgggtacgct
     9121 tggctctgac ggcttgttcg tggtggcaca tcccgactcg aaggcggcca ttcacgacat
     9181 gggcgatcag ttcagctctg acgtggacta ccagaacgga tacgacagca tccagattcg
     9241 atacggcgag accgtggtcg atgcgatcgc gtatgacact ttcccacccg ataaattttt
     9301 cgcaggcgag ggaacggcgg ttccgaagcc ggcgacagac cacagcctgg cccgcgacct
     9361 ttcgcacacc gacaccgaca ataacgcaga cgacttcatc gaacttgcca cacccacccc
     9421 gggcgcggcc aatcaggacg tcaaccagcc gccgatcgcg gaagtcctgt gcccggcgac
     9481 ggccaagctt ggcgaaagcg cgacgttcga tgcctctgga tcggacgacc cggatggttc
     9541 gattgccgaa ttcaagttcg atttcggcga cgacagtccg gccgtttcgg ggcttgattc
     9601 ggtcgtcaat catactttcg gggcggccga cacgttcacc gtcacggtca ccgtcaccga
     9661 caaccgcggg gacaaggaca ccgccacatg ccagatcgtg gttggcgatg acaacgcccc
     9721 gacagtcgat ttcatcaagc cggccaacga cacccaggtg acccagggat cagtcgtcac
     9781 cgtcctggtc aatccccagg cggttcccgg tcgcaacatc gtcagggtcg aactgtgggc
     9841 ggatggtgtc aatacgggca tgtcggacga ctcggcccca tatgagttca catacgtcgt
     9901 gccggaaggt tacccgaaca acacctcact gcagcttcag gccaaggcgg tcgataatct
     9961 cggctcgtcc ggttttggct cggtcaggct gcaggtaaaa aatgatgtgc cgatagccag
    10021 cttcaccgcc gtcgtttccg gcgacaagca gatcaccgtc gatgcttcgt cttcccgtga
    10081 tacggagacg gcgacggcgg atcttcaggt tcgctgggac ttcaccaacg acggaacctg
    10141 ggatacggac tggtcagccg aaaaggtcgt ggtcaaggac tatccggccg atggccagta
    10201 ctcgataaag atggaagtgc gcgatgcgat tgggcagacg tcgtccatgg tcagggacgt
    10261 gacgctgaca tcgattcagt acgtcggcgg aaccgtcacg acaacgacat ggaccggaac
    10321 catcgtaatt acgggcgacg tgaccgttcc cgtcaacgag accttgacgg tggctgcagg
    10381 aacgagcgtg cagttcgtgt atatcgacca gaacggtgac caggtcgggg attacgacat
    10441 caccgtcaac gggaccctga tcgtcaacgg gacacagcag cttccggttc ttttcacttc
    10501 ttacggaacc gaacataaac atgcgagtgc ctggaatcgc atcctgatcc agggtgccgg
    10561 atcacagatt cgcaatgccg cgttcgagta tgc
//
LOCUS       DAOPPA010000045        10522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7188_length_10522_cov_4.396006, whole genome shotgun sequence.
ACCESSION   DAOPPA010000045 DAOPPA010000000
VERSION     DAOPPA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_10522_cov_4.396006"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKH54_02385"
     CDS             complement(<1..662)
                     /locus_tag="PKH54_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOC98763.1"
                     /translation="MINPIDFIQYLADKGSGFFTGIPDSTFKGLTALFDSGAAGFVHL
                     GASNECEAMGIAAGYHLSTGRIPVVYMQNSGFGKTVNPYTSLTARDVYSMPVLMIIGW
                     RGEPGIKDEPQHKMMGRILPDLLRTLEIESVVLDPDDWMQNVDMALDYCRRQNAAFAL
                     VVRSGVFAESPAKDKAPVSTLPLREEILERLVQSSPTDALFISTTGKTSRELYEIRDR
                     LQ"
     gene            complement(659..1963)
                     /gene="aepX"
                     /locus_tag="PKH54_02390"
     CDS             complement(659..1963)
                     /gene="aepX"
                     /locus_tag="PKH54_02390"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOC98764.1"
                     /translation="MSSKVYVAMSADLIHTGHMNILEEARKYGDVMIGLLTDRAIASY
                     KRLPYMTFEQRRKVVENLKGVDVVVPQDTLDYVPNLEKYRPDFVVHGDDWKTGVQKEV
                     RQRVIDTLAKWGGKLIEPIYTPGISSTELNIWLREMGTTPGIRLNMLRRLLESKDTIR
                     VMEAHSGLTGLIVETVKVKKGGMNHEFDAMWISSLTDSTAKGKPDIGCVDLTSRLNTI
                     SDILEVTTKPIIFDGDSGGLSEHFVYMVRTLEREGVSAVIIEDKVGLKKNSLFGTEVE
                     QSQDTIEAFSEKIRAGKKAQITQNFMIFARIESLILQQGMDDAIRRASAYISAGADGI
                     MIHSKEKTPDEIFEFCRRYNEFDVKVPLVVVPSTYSSVTESELAAAGVRVVIYANHLL
                     RSAYPAMVKAARLILENERAHEADDLCMPIKEILTLIPEHKS"
     gene            complement(2095..2451)
                     /locus_tag="PKH54_02395"
     CDS             complement(2095..2451)
                     /locus_tag="PKH54_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOC98765.1"
                     /translation="MLSRILPIFLLVFSNIFMTYAWYGHLKDMRARPLLLAIVASWGV
                     AFFEYCLQVPANRIGFGVYSLGQLKVLQEVITMCVFFFFATLYMKEKLTLDYLWASLC
                     LVAAAYFMFRPHGHQM"
     gene            complement(2515..4197)
                     /locus_tag="PKH54_02400"
     CDS             complement(2515..4197)
                     /locus_tag="PKH54_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98766.1"
                     /translation="MIPYALILIPVAFLLGLVLLVIWARQDAARPLWLAIGALCSAAF
                     AFFWFPDFTDFERDLVILQAGLSALNIDHLFGNGNHFCTTCMQASEVLSRVDGAMLPI
                     EYMVLFNRILHVLNAGFLVIVGLEVTGSLIWPPFFVAGFVGIPIVAVAARTNYPSAFL
                     LTLFFIGLLSGAVFVSRRSGLGAQIAAITALLVVTALAFLTRPDMATPGLAAIAVIIG
                     SDRAVRPILARLARLARTILRLPEPGGRAATGWTSIRDRNWFVASFLAVLVMIALEVA
                     SNMLDERSIPVWLIDGVNLMNPSTANMFFHTFLLLPFSMALLAVAGFARQIRHPAVYL
                     ALPVSMIFLLKTYSSASRYDYHTLFRMFSVILPMILVIACWGLRDLNRLIGRYVPDVR
                     LRLLVAALVIASFSINTPFVRKAMRVHRPPEGYLTAFEEPEGMLGMNPQLEVRFLVDA
                     LRDHPECVLYTKVIGTDGYEDIAAGKSQGEFRDVWITRRGVSDRPPADMPRCQLFYRS
                     LDCNLLGRRACDQDVVHGEPVSEISFSSRPYGDYLLVRYEQPVVLGLYRAGP"
     gene            complement(4275..5468)
                     /locus_tag="PKH54_02405"
     CDS             complement(4275..5468)
                     /locus_tag="PKH54_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC98767.1"
                     /translation="MKRLLRTGFRIPRRDVTAFDDVAASVAGWPNASGACADLAAEFV
                     RRWGYLGAFPCGSGRDGLRMLLRALRPMLPDVAWVPAWTIPAVPQIFTREGFDVRFID
                     TDCGTMSLTADLVERTCTSPGLLLVTHYFGLSADMGAIGRVAARRGLKIVEDCAHAAG
                     VICQGRPAGSRGLGGFLSFETRKPLNGLGGGMVVTNSEAAFELLSRMPPGRRRGLAAD
                     AMKLAVASSEWLMLRRPLFEIASRFLYSDQIRPAVVGLYRKMHSSSRGSEAAFSDRQA
                     RTVLAQLDGLAAAVERKRAVAAIYDRHLPASFSRPSDPADRPHGYYMYVTSHPAANLV
                     GRRLRELGIDCGIGQEVLQMCGDPGSCPGAARLAQTAIELPMYASLTESDALAVCRAA
                     AIAER"
     gene            complement(5465..6448)
                     /locus_tag="PKH54_02410"
     CDS             complement(5465..6448)
                     /locus_tag="PKH54_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98768.1"
                     /translation="MVYPAVSYRRAAAAFVAMASGDPVPLFASLELTLRCNGACDYCG
                     SNRASAAAPSELPTASWMTILDDLARSGCIAVSFTGGEPLLRTDVEALASRAATLGMS
                     VSLNTNGRLLEKRRSILKSVSRVTVSIDGIREVNDMIRGAGAFDAAVAAIRSARFAGV
                     PVSVTAVISAGSARRLPDFLKWLASMDLKAMFQPAYDHMLRSGDIPANPRPDAAVLAT
                     ALDAVAAAIPSGVVLNSRESLEIMSGRGAREVACFGGRFFVRVDHRGCVGVCGLSRDP
                     RHGLPCIDAAGGIVTAMKMIGWPRPACDSCPSAARLEINRFMSAAATRGIR"
     gene            6566..7996
                     /locus_tag="PKH54_02415"
     CDS             6566..7996
                     /locus_tag="PKH54_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98769.1"
                     /translation="MRLACIYVPFGHMPFQENLRVVDEDFGRLPPLSLLYAAAIAENA
                     GHEVLLIDANVSRMPYEEVVAQLEQFKPQAMAFTLSTYAFTSTVEWMGRLKNHFRVPT
                     IAGGINPRLYPVETIANPAIDYCVLHFATRGLPALLDAIEHGRDPAGLPEVVAPGPDG
                     NPITGSVDETLNPYDFLPFPARHLVDNSKYHSFISQRRNFTVAVTSTGCPFNCTFCAI
                     APLPLFQNPVDRVLAEVAECVARYDIHEIDFFDADFFANRKRGIDLCNGIRGLGLDLE
                     WSCRTRVDIINEEVLDAAAASGCRQMYLGIETPDPKAQELMRKRVHVDRVRGVLEMMK
                     KKGIRPLGFFMLGVPGETVLSATQTIRYAMDLPLDYAQFSRMIPKPGSGIHRELVKVI
                     GDDPWKRHILGETRLDRLPNIWSRIDEKTVELLTKLAYISFYYRPGYIIRALARIRSA
                     DELSRSAKTAIRMILDLGHFDENGRK"
     gene            8025..9200
                     /locus_tag="PKH54_02420"
     CDS             8025..9200
                     /locus_tag="PKH54_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC98770.1"
                     /translation="MNGSGTVAAVRCASYEDCAEPILRAIELAGILPELRGRRVLLKP
                     NMMKGVPPERAEATNPVFVGALVRLLKDHGCTVTVGDSSGILGFTSEVFDASGMTEQV
                     TANGGQVINFDAGPFFEIDLGGAFGGVRRPVARALLEADAVLDVPKVKTHTFLELTLS
                     VKNLVGVFPGAVKCGLHTLAPDREVFSGLVAGIPAALQAAGVNIAGSVVDGVLALGGR
                     GGSVPPAPTWMNVVAAGRNLFDVDAWCAHAAGFGMNDVPLCRIGAAAGFGAASVEAIH
                     VVGDVIEPVRLKRPGRDIQETSTVLTSAYYKARGRLVRPVFDGGRCTDCGRCDEVCPE
                     RCIVVVGPRDRRISDACTRCLACREVCPSGAMGLVANRALKPFLKSRARGLKMDRLR"
     gene            9221..>10522
                     /locus_tag="PKH54_02425"
     CDS             9221..>10522
                     /locus_tag="PKH54_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98771.1"
                     /translation="MNDLCPTVLVNPGIVQAGPVAQKVFPPIGLMYLVAALKQAGLPV
                     RLIDANATGEDDDAVVSRLQAMQPLAVGLPVFDITLVQTARLARAIRAGFPRVILFAG
                     GPAATVDPARTIAMIPEIDYVLAGEAEESIVRFVRGLRAGEVPSGIPGLFAAGHNLPD
                     DHPPQPARILDLQAIPLPDRECVRENYEARRYYTVLVPDRHIDCVVTSRGCPHRCAFC
                     YNWRFRQAYRSPDSCLEEFEMLRARGVTTIEILDDNFTTDRDRAMTILESVAAMRMPV
                     RFRIKARADAIDEGLMKAARRAGVYQVSIGAESGSPEMLCRMKKRLTPDVTARAVETV
                     MKSGAYCHTSFIVGFPGETRQTMDDTVKMIKAVRPTSVSIDVLAPYQGTSVYEMAKAD
                     GTLQGDWSASPGDPLPWIRLDWTTTRKDLEDARKAMLFRVYF"
BASE COUNT         2266 a         3196 c         3119 g         1941 t
ORIGIN      
        1 atttgcagcc tgtcccgtat ttcatacaac tccctggatg tcttgccggt tgttgagatg
       61 aaaagcgcat ctgtcggaga cgactgtaca agcctttcca ggatttcctc ccgcagcgga
      121 agtgtgctca cgggcgcctt gtctttcgct ggggattccg caaacacgcc actccggacc
      181 accagagcga atgcggcatt ttgcctgcga cagtaatcaa gcgccatgtc aacgttttgc
      241 atccaatcgt ccggatcaag cacgacactt tcaatttcaa gggttctcag cagatccgga
      301 agaattctgc ccatcatctt gtgctgaggt tcgtccttta tcccgggctc gccacgccag
      361 ccgataatca tcagtaccgg catcgaatag acgtctctcg cggtcaagga cgtgtacggg
      421 ttaacagtct tgccgaatcc ggaattctgc atatatacga ccggaatcct gcccgtggaa
      481 aggtgatacc cagccgcaat tcccatggct tcacactcgt tcgaggcccc aaggtgaaca
      541 aagcccgcag cgccagagtc gaacaaagcg gtcagcccct taaacgtcga gtcgggtatc
      601 cccgtgaaga agccggaacc tttgtcggcc agatattgaa taaaatcgat tggattaatc
      661 atgacttgtg ctccggtatc aacgtcagaa tctcctttat gggcatacag aggtcatccg
      721 cctcatgagc cctttcgttt tcaaggatca atcttgctgc cttaaccatc gccggatatg
      781 cactgcgcag caagtgattg gcatatatga caacccgcac accggcagcc gccaattcag
      841 attcggtaac cgaactgtag gttgatggaa caacgacaag cggcaccttg acatcgaact
      901 cgttgtaccg ccggcagaac tcgaaaatct catcgggcgt tttttccttg ctatggatca
      961 tgatcccgtc agccccggcc gagatatagg cacttgcccg tcgaatcgcg tcatccatcc
     1021 cctgttgaag aatcaggctc tcgattcgtg caaagatcat gaagttctgg gtgatctgag
     1081 cctttttacc ggcccggatc ttttccgaaa aggcctcgat ggtgtcctgg ctctgttcaa
     1141 cctcggttcc aaaaagagag ttcttcttca ggccaacctt gtcctcaata attactgcgg
     1201 aaaccccctc gcgctcgaga gtccgcacca tgtaaacgaa atgttcggaa agcccgccgg
     1261 aatcaccatc aaaaatgatg ggcttggtgg ttacttccag aatatcggaa atcgtattca
     1321 gccttgacgt caaatcaacg catccaatat ccggctttcc ttttgccgtc gagtccgtaa
     1381 ggctgctgat ccacattgcg tcaaactcat ggttcatccc gcccttcttg actttaacgg
     1441 tttccactat cagaccggtc aggccgctgt gtgcctccat cacccgaatt gtgtcctttg
     1501 attccagcag acgcctgagc atattcaatc tgataccggg cgtagtcccc atctccctga
     1561 gccagatatt caattcagtg gaggaaatgc cgggcgtata gatcggttca atcaactttc
     1621 cgccccactt cgcaagcgta tcaataaccc gctggcgaac ctccttctgg acgccggttt
     1681 tccaatcgtc accatggacg acaaagtccg gcctgtactt ttcaaggttt ggcacatagt
     1741 ccagcgtatc ctggggcacc acgacatcaa cacccttaag attctcgacc accttgcgac
     1801 gctgttcaaa agtcatgtat ggcagacgct tgtaactggc gatcgccctg tcggtgagca
     1861 gaccaatcat cacatccccg tacttgcgcg cctcttccag aatattcatg tgtcccgtat
     1921 ggataagatc tgcactcatg gccacataaa ccttactgct catcctgtcc gctccttgaa
     1981 atgaccgcag aaagcgtatc gacgccattg cgcggccaag aatatcacta ataaaatgcc
     2041 gaagccaacc gggcttgaat cggcaaccct ggcggaaaaa cgccacataa tcgttcacat
     2101 ctgatggccg tgcggcctga acatgaagta tgcggccgcg accaggcaca gcgacgccca
     2161 caggtaatcc agggtcagct tttccttcat gtagagcgtc gcgaagaaga agaacacgca
     2221 catcgtgatc acttcctgga gcaccttcaa ctgccccagg gaatagactc cgaaaccgat
     2281 cctgttcgcc ggcacctgaa ggcagtattc aaaaaaggcc acgccccagc ttgcgacgat
     2341 cgccagcagc aacggccgcg cccgcatatc cttcaggtgg ccgtaccagg cgtacgtcat
     2401 gaagatgttg gaaaacacca gaagaaatat cggcaggatc cgtgaaagca tctgaagcaa
     2461 tccttcgcaa gacagtcccg gtctatttgc tccagaagac ggacgccgtc aagattacgg
     2521 cccggcacgg taaagaccga gcaccacggg ttgctcgtac cgcaccagaa ggtagtcccc
     2581 gtatgggcgg ctggagaacg atatctcgct gaccggctcg ccgtggacga catcctgatc
     2641 gcaggcacgg cgccccagga gattgcagtc aagcgaccgg tagaatagct ggcaccgcgg
     2701 catgtcggcc ggcggtcgat cggaaacgcc gcgcctggta atccagacat ccctgaattc
     2761 gccctgggat tttcccgccg cgatatcctc gtatccatcc gtgccgatga ccttcgtgta
     2821 aagaacgcat tcgggatgat cccgcagggc atccaccaga aagcgcacct cgagttgggg
     2881 gttcatgccc agcatccctt ccggttcctc gaaggccgtc aggtaacctt cgggtggacg
     2941 gtgaacccgc atcgccttgc ggacaaaagg cgtatttatt gaaaaagatg cgattaccag
     3001 cgccgcgaca agcagccgaa gacggacgtc cgggacgtac cgtccaatca ggcggttcag
     3061 atcccttaac ccccagcagg caatgaccag gatcatcggc aggatcaccg aaaacatcct
     3121 gaacagcgtg tgatagtcgt atcgggacgc cgacgagtac gtcttcagca ggaaaatcat
     3181 cgaaaccgga agggccagat aaactgccgg gtggcggatc tggcgggcga acccggcgac
     3241 ggcaagcagg gccatcgaga atggcaacag caggaacgtg tggaagaaca tgttggccgt
     3301 gctcgggttc atcaggttga cgccgtcaat cagccagacc gggatcgatc gctcgtcaag
     3361 catgttgctg gcgacctcga gggcgatcat gaccaggacg gccaggaacg acgcgacgaa
     3421 ccagttgcgg tcgcgaatcg acgtccagcc ggtggcggca cggccgcctg gctcgggcag
     3481 gcgcaggatg gttcgggcaa ggcgcgccag gcgtgcaagg atcggacgga cggccctgtc
     3541 ggatccgatg atgaccgcaa ttgccgccag tccgggggtt gccatgtcgg gacgtgtcag
     3601 gaaggcaagc gcggtgacga caagcaatgc ggtaattgcc gcaatctggg cccccagtcc
     3661 cgaacggcgc gaaacgaaga ccgcgccgga aagcaggccg atgaagaaca gcgtcaaaag
     3721 gaaggccgac ggatagttgg tgcgcgccgc caccgcgaca atcgggatgc cgacaaaacc
     3781 ggcgacgaag aaaggcggcc agatcaggct tcccgtcact tccaggccga cgatgacaag
     3841 gaagccggcg ttcaggacgt gaagaatacg gttgaacagc accatgtatt cgatcggcag
     3901 catggcgccg tcaacacggg aaagtacttc ggaagcctgc atgcacgtgg tgcagaaatg
     3961 attgccgttg ccgaacaggt ggtcgatgtt cagcgcgctc aatccggcct gaaggattac
     4021 caggtcacgt tcgaaatctg tgaagtccgg gaaccagaaa aaggcgaacg ccgcggaaca
     4081 gagtgccccg atggccagcc agagcgggcg ggccgcgtcc tgtctggccc agatgacaag
     4141 aagaaccagg ccaagaagga atgcgacagg gatcaggata agcgcgtaag ggatcatcgg
     4201 ttgttccttc gggatcggcc cgatggcggc cgccggcaag gcaaacatat accacaggag
     4261 aaccttgcgg tcagctatcg ttcggcgatg gccgcggccc gacacacagc cagcgcgtcg
     4321 ctttcggtca gggatgcgta catcggaagc tcgattgccg tttgggcaag ccttgcagcc
     4381 cccgggcagc ttccaggatc cccgcacatc tgaagcacct cctggccgat tccacagtcg
     4441 atgccaagct cgcgaaggcg acgaccgaca aggttcgccg cggggtgcga ggtcacgtac
     4501 atgtagtaac cgtgcggacg gtcggcagga tccgacggtc gcgaaaacga cgcgggaaga
     4561 tgacggtcgt aaatggccgc cacggccctc ttccgttcaa ccgcggcggc cagcccgtcg
     4621 agttgggcca ggacggtcct ggcctgacga tccgaaaagg cagcctcgct gccacggctg
     4681 cttgaatgca tcttccggta aaggccgaca acggcgggac ggatctgatc cgaatacagg
     4741 aacctggaag cgatttcgaa aagcggacgg cgcagcatca gccattcgga ggaggccacc
     4801 gccagcttca tcgcatccgc cgcaaggccg cgccggcggc cgggcggcat ccttgagagc
     4861 agttcgaacg cggcttcgct gtttgtcacg accattccgc cgccaagtcc gttaagcggc
     4921 ttcctggttt caaacgaaag gaagccacca agcccgcggg aacctgccgg acggccctga
     4981 caaatcaccc cggccgcgtg ggcgcagtcc tcgacaatct tcaggccgcg ccgggcggcg
     5041 acccgcccga tggcgcccat gtcggccgaa aggccgaagt aatgcgtgac caggagcagt
     5101 ccgggcgacg tgcaagtcct ttcaaccagg tctgccgtca gcgacatcgt cccgcagtcg
     5161 gtatcgatga acctgacgtc aaaaccttcc cgggtaaaaa tctgcggaac cgccggtatt
     5221 gtccaggcgg gaacccacgc gacatccggc agcatcggcc gcagggcccg cagcagcatc
     5281 cgaaggccgt cacgcccgga tccgcaaggg aaggccccaa ggtaccccca ccgcctgacg
     5341 aactcggcgg caaggtcggc gcaggcgcca gaggcgttcg gccagccggc caccgacgcg
     5401 gcgacatcgt caaacgcagt gacatccctg cgcggtatcc ggaagcccgt ccgaagcagc
     5461 cttttcatcg aattcccctt gtggcggcgg ccgacatgaa acgattgatt tccagccggg
     5521 ccgcgctggg acacgaatca caggccggac ggggccatcc aatcatcttc atcgccgtga
     5581 cgatgccgcc ggccgcgtcg atgcacggca gcccgtggcg tggatcgcgg gacaggccgc
     5641 aaaccccgac gcagccacga tgatcgacac gaacgaaaaa gcggccgcca aaacatgcga
     5701 cttcgcgcgc gccacgaccg gacatgattt ccagggattc gcgtgaattc aggacgacgc
     5761 cggatggaat cgccgcggca accgcatcaa gcgcggtcgc aagcacggcg gcatcaggcc
     5821 ggggattggc tggaatgtca cctgaacgca gcatgtggtc gtaggccggc tggaacatcg
     5881 ccttcaagtc catcgaagcc agccacttca ggaaatccgg caggcgccgg gccgacccgg
     5941 ccgaaatcac ggccgtgacg gacaccggga cgcctgcgaa cctggccgac ctgattgccg
     6001 ccaccgccgc gtcgaaagcg cccgcaccac gtatcatgtc attgacttca cggatgccgt
     6061 cgatgcttac agtgaccctg gaaaccgact tcaggatcga tcgtcgtttc tcaagcagcc
     6121 gcccgttcgt attcaggctg acggacatgc ccagcgtggc cgccctggac gccagggcct
     6181 cgacgtcggt tcgaagaagc ggctcaccgc cggtgaagct gaccgcgatg caaccagacc
     6241 gcgcaagatc gtcaagaatg gtcatccagg aggctgtggg caattcagag ggcgccgccg
     6301 cggatgcccg gttcgatccg caataatcgc acgccccatt gcatcgaagg gtaagttcca
     6361 ggcttgcaaa aagcggaacc gggtcgccgg atgccatcgc gacaaacgcc gcggcggccc
     6421 gacgataaga aactgccgga tataccacag gaccccccgc cttttcgacg ccaggactca
     6481 caactttggt aagatatccg tcgcgaggcg cctcggcaac accattgttc catcgcccgc
     6541 ttcgcgaccg gattgggggg attgattgag acttgcctgc atttacgtcc cgttcggaca
     6601 catgcctttc caggaaaacc tgcgggtggt cgatgaggac ttcgggcgac tgccgccgct
     6661 gagcctgctt tatgccgcgg cgatcgccga aaacgccggc catgaagtac tgctgatcga
     6721 cgccaacgtt tcaaggatgc cttacgaaga agtcgtcgcg caacttgagc agttcaagcc
     6781 ccaggccatg gcgttcacgc tttcgacata cgcgttcaca tcgacagttg agtggatggg
     6841 gcggctgaag aaccatttcc gggttccgac catcgccggc ggtatcaacc cgcgcctgta
     6901 tcccgtcgaa acaattgcca atccggcgat tgattactgc gtgcttcact tcgcgacgcg
     6961 cggcttgccg gcgctgcttg acgcaatcga acacggacgc gatccggccg gcctcccgga
     7021 agtcgtggcc cccggaccgg acgggaaccc gatcaccggc tctgtcgatg agactttgaa
     7081 cccatatgac ttcctgccct tcccggcacg ccacctggtc gacaattcga aatatcactc
     7141 cttcatatcg caacgccgga acttcacggt cgcggtcaca tcgacagggt gcccgttcaa
     7201 ctgcactttc tgcgcaatcg ccccgctgcc actgtttcaa aaccctgttg atcgggtgct
     7261 tgcggaagtg gccgaatgcg tagccaggta cgacatccac gaaatcgact tcttcgatgc
     7321 cgacttcttt gccaaccgta aacgtgggat cgatctgtgc aacgggatcc gggggcttgg
     7381 ccttgatctg gaatggtcgt gcagaacccg agtcgatatc atcaacgagg aagtcctgga
     7441 cgcggccgcg gcatctggct gcaggcagat gtaccttggc atcgaaaccc cggatccgaa
     7501 ggcccaggaa ctgatgcgca agcgggttca cgtggatcgt gtccgcggcg tgcttgaaat
     7561 gatgaagaaa aagggcatcc gcccgcttgg tttcttcatg ctgggcgttc ctggcgagac
     7621 ggtcctttcg gcaacccaga cgatccggta cgccatggat ctgcctctgg attacgccca
     7681 gttttcgcgg atgatcccga agcccggcag cggaatccat cgtgaactgg tcaaggtaat
     7741 cggcgatgac ccgtggaaga ggcacatcct tggcgaaaca cgacttgatc ggctgcccaa
     7801 catctggtcc aggatcgatg aaaagacggt cgaactgctt acgaaactgg catacatttc
     7861 cttctactac aggcctggat acataatcag ggcgcttgcc aggatccgca gcgcggacga
     7921 actctcgcgc agcgcaaaga ccgccatcag gatgattctg gatcttggcc acttcgatga
     7981 aaacgggcgc aagtgacgtc cggccatgcg gcaggggttg attcatgaac ggttccggca
     8041 cggttgcggc cgtacgctgc gcttcatacg aagactgcgc cgaaccgata ctgcgcgcaa
     8101 tcgagcttgc cggaatcctg ccggaactgc ggggccgacg cgtcctgctg aagccaaaca
     8161 tgatgaaggg ggtacctcct gaacgggccg aggcgaccaa tccggtcttc gtcggtgccc
     8221 tggttcgcct gctgaaagat catgggtgca cggtaacggt tggtgattca agcggaatcc
     8281 ttggattcac cagcgaagtc ttcgatgcct cgggaatgac ggaacaggtg acggccaacg
     8341 gcggtcaggt gatcaacttc gacgccggcc ctttcttcga gatcgacctt ggaggcgcct
     8401 ttggcggcgt gcgccggccg gtggcaaggg ctttgctgga agcggacgcc gtgctggacg
     8461 tcccgaaggt gaagacccac acgttcctgg aactcaccct ttccgtgaag aacctggtgg
     8521 gtgtctttcc cggtgcggtc aagtgcggcc ttcatacgct ggccccggat cgcgaggtct
     8581 tctcgggact ggtggccggc attccagccg ccctgcaggc ggccggcgtc aacatcgccg
     8641 gctcggtcgt cgatggtgtg ctggcccttg gaggcagggg aggatcggtt cctccggcac
     8701 caacctggat gaacgtggtg gccgccggca ggaacctttt cgatgttgac gcgtggtgcg
     8761 cgcacgccgc cggtttcgga atgaacgatg tcccgctttg caggatcggc gccgcggccg
     8821 ggttcggggc ggcgtccgtt gaagcaatcc atgtcgtcgg cgatgtaatc gaaccggttc
     8881 gattgaagag gccgggacgc gatatccagg aaacatcgac cgtcctgaca agcgcatact
     8941 acaaggcgcg cggcaggctg gtcaggccgg ttttcgacgg cggtcgttgc accgactgcg
     9001 gcaggtgcga cgaagtatgc cccgagcggt gcatcgtggt cgtcgggcca cgggatcgca
     9061 ggatttccga cgcttgcacg cgctgcctgg cttgccggga ggtctgtccg tcgggcgcga
     9121 tgggacttgt cgcgaaccgg gcgctgaagc cattcctgaa aagccgcgcg cgcggcctta
     9181 agatggatcg gctgcgctga agcggcctgc gggggacgaa ttgaacgacc tttgcccgac
     9241 cgtgcttgtg aatccaggaa tcgtccaggc ggggcctgtc gcgcagaagg tatttccacc
     9301 gattggcctg atgtacctgg tcgcggccct gaagcaggca ggcttgccgg ttcgcctgat
     9361 cgacgcgaac gcgaccggtg aggacgacga cgcggtcgtt tcacggctgc aagcgatgca
     9421 gcccctggcg gtcgggcttc cggtcttcga catcacgctt gtgcagaccg ctcggctggc
     9481 gcgggccatc cgtgccgggt ttccacgcgt gatccttttc gcgggcggtc cggccgccac
     9541 ggtcgatccc gcgcggacaa tcgccatgat tccggaaatc gattacgtgc tggcgggcga
     9601 ggccgaggaa tcaatcgtcc ggtttgtcag gggcctgcgg gccggcgagg tcccgtccgg
     9661 aatccccggc cttttcgctg ccgggcacaa tctgccggac gaccacccgc cccagccggc
     9721 caggatactc gaccttcagg caattcccct gccggatcgg gaatgcgtgc gggaaaacta
     9781 cgaggccagg cgatactaca cggtactcgt cccggatcgg cacatcgact gcgtggtcac
     9841 cagccgcggc tgcccccacc gatgcgcgtt ctgttacaac tggcggttca ggcaggcata
     9901 ccgatcgccc gacagttgcc ttgaagaatt cgagatgctg cgcgcgcgcg gcgttacaac
     9961 catcgaaatc cttgatgaca atttcaccac cgaccgggat cgggcaatga cgatcctcga
    10021 atcggtcgcc gcgatgagga tgccggtacg gttcaggatc aaggcacgcg cggacgcgat
    10081 cgatgaagga ctgatgaagg cggcaaggcg ggccggcgtc tatcaggtca gcatcggcgc
    10141 ggagtcgggt tcccccgaga tgctctgccg gatgaagaag cgcctgacgc cggacgtgac
    10201 ggcgcgcgcg gtcgaaaccg tgatgaagtc gggcgcgtac tgccatacca gcttcatcgt
    10261 gggatttccc ggcgagacgc gccagacgat ggacgacacg gtgaagatga tcaaggcggt
    10321 caggccgaca tcggtctcga tcgacgtgct ggccccttat cagggaacat cggtttatga
    10381 aatggcgaag gccgacggga cgctccaggg cgactggtca gccagtccgg gtgacccgct
    10441 gccatggatc cgcctcgact ggacgacgac caggaaggat cttgaagacg cccgcaaggc
    10501 catgctcttc agggtatatt tc
//
LOCUS       DAOPPA010000046        10506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7201_length_10506_cov_3.857143, whole genome shotgun sequence.
ACCESSION   DAOPPA010000046 DAOPPA010000000
VERSION     DAOPPA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_10506_cov_3.857143"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKH54_02430"
     CDS             <1..353
                     /locus_tag="PKH54_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98772.1"
                     /translation="SATFNKNKDKVFRTPIGEFVYRYLPAAVYPHGIKHEIEEGAGYL
                     IATPEKALCDSIYKVKGLNSPRDVVNLLFDDWRIQQSDLANLDRAFISWIAPMYGRRS
                     VAHLVKALETGDWR"
     gene            350..1225
                     /locus_tag="PKH54_02435"
     CDS             350..1225
                     /locus_tag="PKH54_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOC98773.1"
                     /translation="MTDNGPVGQMLEAYQCRNNDQRRSALKEIVQEITLVALSRNGFF
                     NRGAFYGGTALRIFHGLGRFSEDLDFSLLEPDPAFDLGLWLEPVRQELASFGFDMEVS
                     RKAKSAAGAVQSAFIKGGTLIHLVRIVGMSPPVSGVPAGEMLKIKFEVDTDPPAGATF
                     EIKYRLLPIPYSVRLYDLPSLFAGKIHAVLCRNWNQRVKGRDFFDYLWLLGRGVSVNL
                     PHLEARMRQSGHLIDAGPLDRDRLVVLLKDRFSRVDYEQARRDIAPYIADSRLVEAWS
                     RDLFTTVTSEQLKCS"
     gene            complement(1359..1817)
                     /gene="ruvX"
                     /locus_tag="PKH54_02440"
     CDS             complement(1359..1817)
                     /gene="ruvX"
                     /locus_tag="PKH54_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOC98774.1"
                     /translation="MRVMAVDPGTVRCGIAVCDWLGMLASPVGIIRVKDGRELAAAIA
                     AKAVELEAGLIIVGYPIQMDGTAGHRALAAERLAEQIRRHTDLEVRLFDERLTSAEAE
                     DLLKEAGRTIKESKDMRDAAAAAVLLSWWLDKNPQSAPRDPDQDPSDLNF"
     gene            complement(1814..2467)
                     /locus_tag="PKH54_02445"
     CDS             complement(1814..2467)
                     /locus_tag="PKH54_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOC98775.1"
                     /translation="MMLTREQIAMRVAQEVKDGYYVNLGIGLPTLVSNYIPENIQVVF
                     QAENGLIGVGPFPYEGDQDADLINAGKQTVTAMPGAAFVDSCECFGMIRGGHIDMTVL
                     GAMQVGANGDLANWAVPGKLVKGMGGAMDLVSGVKHVVVAMEHTAKGAHKIVEKCTLP
                     LTGVRCVNKIITDMAVMEVTPDGLVLQETAPGVTVDEIRAATGASFKVAPDLKEMAL"
     gene            complement(2482..3186)
                     /locus_tag="PKH54_02450"
     CDS             complement(2482..3186)
                     /locus_tag="PKH54_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOC98776.1"
                     /translation="MKKLLETADAAVADINDGAVVAVAGFGLCGIPENLIDALVRKGS
                     KHLTLISNTCGTDNQGVGLLIANGQVDHVIASYVGENAVVERMAAEGTLTYDLMPQGS
                     IAEALRCGGMGIPVFFTPAGVGTVRAEGRETRTFDGRDYLMEKALKPDFSLVKAYRGD
                     PTGNLRFRYTARNFNPCMAMAGKVSIAEVEIMEAVGGIEPDDIHLAGIYVDRIVLGTE
                     YRKPIERLTTRPRQAC"
     gene            complement(3295..6126)
                     /locus_tag="PKH54_02455"
     CDS             complement(3295..6126)
                     /locus_tag="PKH54_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98777.1"
                     /translation="MNCRTRIAIPTIATLILAAGCAESTPAPCPECAVPVEWTVAISP
                     TAPEAVKLAADDVVKYLGLMGRSAVLDDANDSTKCRDGVGRVVFIGDGIGEAVFDTEA
                     PTDQTFRFQETWCGSGILIELSGGGLLGRQYAAYDWLHGLGIRFFHPEQEYVPAEMAK
                     PAEPAFREFTPAFKYRSVSLHLTHPLEMGDIFTLGKAEHLSDGINYIDWCVKNLASDG
                     RGGIPVEGYEQYGIMRGFTTSAGFRLYGMQQGNHGLIDPDDPRPADQQITDKITELMG
                     TDPDNYPDFFSFNFDPTEFTEMDDQLVVHQMTVIADFIDQNYPGVQIVTTNHGTYGEP
                     TAHYGVRYFNLPQFAPESIGVKCHTLMFYDLERPAPVYGNTDFHFLYDFMEQEYQKRQ
                     LWYFPESAWWLTFDNQLPLYLPITIEARDRDIQKLKHMLSGKLVGHHVFGTGHEWGYW
                     QNEYCSFRMSADTDYRWTDCLEDITMPMGTAAGEVRAVMEDVVAMQENEMIYGHILRY
                     LVGTDPETEAAASIGVVFHELPPPPKAIMNWTKEEAESWQAVEAAELATMVAGYEALV
                     ARLEAVRADVPGPAAPFFDEILDGIKVTGLRAKHQLLVYGAVVKFRLAQLNLDEAMQT
                     AARADLEAAIQVTADAKAVIAAREQQFRYQPLARSIGGGENCDQDENWTTYGFRVHCR
                     THNAYYWTRIDELAAEVILSTSQAVSLQDAFLTVNQNFQVAVTDAAVGAVDIDFGDGS
                     AVQNGTSFTHAYATTGTYRIHVSGSRDGTEFTWSADVVAVESKTMTEFTGEILKPTGM
                     EIVTSVMPNIVFGRIDADTVAVGYSAFHEGYVESGRFSILADNADPIFGGVAETLLVP
                     IITKSDNSVLAELTVKAGSVGTADEGATLVIQGQMVTQSVVNAIIAVAGGGFDEAGAR
                     EVVASTIGYTPDTLPESVEFIIHYEIPTE"
     gene            complement(6329..8176)
                     /locus_tag="PKH54_02460"
     CDS             complement(6329..8176)
                     /locus_tag="PKH54_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC98778.1"
                     /translation="MKRLLSSVTLIAFAAAMATSGVMGCGDSETPVSDTTDDSTMPED
                     VADDSTAPEDVAGDVAHQTDATGDAATDAVIPPAVTRFVFVSDIHFRTVEGQEKHSKD
                     IGDLIPMINALSGDYDILVTGGDNFDHVLPAWDEKPLESPMAWFVEEMDKLTMPWVAA
                     IGNHEFYDYFGDVPTLTTDGARRGQAFSTVMGHPLYFTTEINGVKLIVLDSMEDGSWG
                     ETHGLMGKFSQTQLDWLRAELAQGKPSFLFFHHPPSSLSPNAPGGQLCSVVEEYPGVV
                     KSIFTGHTHGFWRGDYCGAPYYVVEDYHSQVNLWYEIEYDAGTDTVTIRNQEYIDFPE
                     MPEFTCEPGESTIADHELAIGTVQKLDVTNGVSDAEGLGEMLGEGLAQIPFVISFDGY
                     EDGTGYASRMTIASRWEIDNFLTYVAGSPCVEMGIHWKGGEPCFEAGPFNIQAQLISF
                     LSSVSEDPVNPDWKANLDIRNMVFEGCIRNDDNGIPVIADGLVYATILRDPTIADLSR
                     ILVDEYCAGRIEGCTPGADSLPACPGTLDVGTYESIFADIRYSCDVQVMGFGLVSLID
                     MLATLPDETHVTGQISTSVITVSDTPEEGGQASSDLFNAEPGHNCAITD"
     gene            8414..9421
                     /gene="pheS"
                     /locus_tag="PKH54_02465"
     CDS             8414..9421
                     /gene="pheS"
                     /locus_tag="PKH54_02465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOC98779.1"
                     /translation="MEELKNEALAQIAQATTTAALKEVEVHWLGKKGLITERLKTLGT
                     LAPEERKNAGRDLNILKNEIQTAIAERTVTVAASEIQQQAASLFIDTSMPGIRARSGH
                     LHPLTMVTEQVVDVFERMGFFTLDYPEAESEFFNFDGLNIPSDHPARDTQDTFYLTDG
                     NLLRTHTSPGQVRAMREFAPPFKAIFPGRVFRCEATDACHEHTFNQVEGIMIDRSVSV
                     ANLISSMMTLLSGVFGRQVVVRLRPGFFPFVEPGFELDIQCMVCGGSGCPVCKRSGWV
                     ELMPCGLIHPNVIRAGGLDPAEWEGWAFGLGLSRLVMMRYEIDDIRWLMSGDLRFVRQ
                     F"
     gene            9459..>10506
                     /locus_tag="PKH54_02470"
     CDS             9459..>10506
                     /locus_tag="PKH54_02470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOC98780.1"
                     /translation="MKYSWNWLSDYVDLSGIDPMTVAGRFTMTVAELEGVEQVGEGLE
                     GVVVGRIVNVADHPDSAKLKLIDVDVGAAGGVVSGVSGAPNVKAGILVPIALPGVVLP
                     GGVEVRAVEVRGVASALVALSEKEMGISDDHGGIMELPADSVPGARLVDTIPVRDTVF
                     EVDNKSITHRPDLWGHYGVAREVAAMTGRTLKPLDETIPQGQADLMTVRVDEPIDCPR
                     YMGMCFDGVGIARSPFWIRHRLRAVGHRPINNVVDLTNYIMCAVGEPMHAFDRRNVGG
                     DTIIVRRAAEGEPFRTLDGQDHVLSAEDLLIADASRGVALAGVMGGENSEVLDDTSTL
                     FIECATFHPGRVRRT"
BASE COUNT         1928 a         3115 c         3207 g         2256 t
ORIGIN      
        1 cttcggcaac cttcaacaag aacaaggaca aggtgttccg tacccccatc ggggaattcg
       61 tttaccggta tcttccggcg gctgtttacc cccatggcat caaacatgag atcgaagaag
      121 gggctggata tttgatcgcg acccccgaaa aggccctttg tgattcgata tataaggtca
      181 agggtctgaa ttcgccgcgt gacgtcgtga accttctgtt tgatgactgg cgaatacagc
      241 aatcggatct ggccaacctg gatcgggcgt tcatatcctg gattgccccg atgtatggaa
      301 ggaggtcagt tgcccatctg gtcaaggcac ttgaaacggg ggactggaga tgactgacaa
      361 cgggcctgtc gggcagatgc tggaagctta tcagtgcaga aacaatgatc agcgtcgatc
      421 ggcgttgaag gagatcgtcc aggaaatcac gcttgttgcc ctttcccgta acggattttt
      481 caaccggggg gccttttacg gaggaacggc tctgcggata tttcacgggc tgggcagatt
      541 ttccgaggat ctcgattttt cccttctcga accagatccg gcctttgacc tgggcctgtg
      601 gctcgagccc gtccgccagg aactcgcctc tttcggtttt gacatggagg tgagcaggaa
      661 ggcgaagtcg gccgccggtg ccgtccagtc ggcattcatc aagggcggaa cgcttattca
      721 tctggtcagg atcgtgggaa tgtcgccccc ggtttccggt gttcctgccg gggagatgct
      781 taagattaag ttcgaagtcg ataccgaccc gcccgccggt gcgacatttg aaatcaagta
      841 ccgactcctg cccattccgt actctgtcag gctctatgat ctgccgtcat tgtttgccgg
      901 caagattcat gcggtacttt gccgtaattg gaatcagagg gtcaagggac gtgatttttt
      961 cgactatctc tggttgcttg ggcgtggagt atccgtcaac ctgccgcacc ttgaagcccg
     1021 gatgcgtcag agcggccatc tgatcgatgc cgggccactt gatcgggatc ggctggtcgt
     1081 tctcttgaag gaccgttttt cccgtgtcga ttatgaacag gccagaaggg atatcgcccc
     1141 atacatcgct gattcccgtc ttgtcgaagc ctggtcccgg gatctgttca cgacagtcac
     1201 gtcggaacaa ttgaagtgtt cataatggaa taaccggccc caggtacacc gccgcagggg
     1261 cgaaagaccc ttctgcgggc ccttcgacag gcccttcggc gggctcaggg caggctcagg
     1321 gcagtaaatt cgcccgccca ccaaagagtc accttttgtc agaaatttag atccgaaggg
     1381 tcttggtctg ggtcgcgggg tgctgattgc gggttcttgt ccagccacca cgaaagcagg
     1441 acggcggccg cggcggcatc gcgcatatcc ttggattcct tgatggttct gccggcttcc
     1501 tttaacaggt cctcggcctc ggccgacgtc agacgttcgt cgaaaagccg tacttcaagg
     1561 tcggtgtgcc ggcgaatctg ttcggcaagc cgttccgcgg ccagtgcgcg gtgaccggct
     1621 gttccgtcca tctgtatcgg gtacccgacg attatcaggc cggcttcgag ttcgactgcc
     1681 ttcgcggcga tggctgcggc cagttcccga ccgtccttca cgcggatgat tccgacgggc
     1741 gacgccagca tcccaagcca gtcgcacacg gctatgccgc agcggaccgt tccgggatcg
     1801 accgccatca ccctcataag gccatttcct tcagatccgg ggcgaccttg aaggatgcgc
     1861 cggttgccgc gcggatttca tcgaccgtca cgccgggggc cgtttcctgc agcaccagtc
     1921 cgtcaggcgt cacttccatc acggccatgt ccgtgatgat cttgttgacg cagcggacgc
     1981 cggtcagggg cagggtgcac ttttcgacga tcttgtgcgc cccctttgcc gtgtgttcca
     2041 tcgcgacgac cacgtgcttg acgccgctga ccaggtccat ggcgccgccc atgcccttga
     2101 ccagcttgcc gggcaccgcc cagttcgcca ggtcgccgtt ggcgccgacc tgcatcgcgc
     2161 ccagcaccgt catgtctatg tggccgccac gaatcattcc gaagcattcg caactgtcaa
     2221 cgaacgccgc gccgggcatc gccgtgacgg tctgcttgcc ggcgttgata aggtcggcgt
     2281 cctggtcgcc ctcgtacggg aacggaccga cgccgatcag gccgttttcg gcctggaaca
     2341 cgacctggat gttttcaggg atgtagtttg aaacaagcgt cggcaggccg attccaaggt
     2401 tgacgtagta gccgtccttc acttcctggg cgacgcgcat ggcaatctgt tcacgagtca
     2461 gcatgatatt cctccgtccc gtcagcaggc ctgccgcggc cttgttgtaa gcctttcgat
     2521 cggcttccgg tattcggtgc ccaagacgat ccgatcgacg tagatgccag ccaggtggat
     2581 gtcgtcgggc tcgattccgc caaccgcttc catgatttcg acttcggcga tgctgacctt
     2641 gcccgccatc gccatgcacg ggttgaagtt gcgcgccgtg tagcggaagc gcaggttgcc
     2701 ggtcgggtcg ccgcggtatg ctttcaccag gctgaagtcc ggcttcagcg ccttttccat
     2761 caggtagtct cgtccgtcga acgtcctggt ctcgcggccc tcggcgcgca cggtgccgac
     2821 gcccgccggg gtgaagaaca cggggatgcc catgccgccg cagcgaaggg cctcggcgat
     2881 cgacccctgg ggcatcaggt cgtatgtcag ggtgccttcg gcggccatgc gctcgacgac
     2941 ggcgttttcg cccacatagc tggcgattac gtggtcgacc tggccgttgg cgatcagcag
     3001 gccgacgccc tggttgtcgg tgccgcaggt gttgctgata agtgtcaggt gcttcgagcc
     3061 cttcctgacc agtgcgtcga taagattttc cgggattccg cacagcccga acccggcaac
     3121 ggcgacgacc gcgccgtcgt tgatgtcggc aaccgccgca tcggccgttt caagaagttt
     3181 cttcattggt cctccaaacg ggaaaacgca ggatggccaa atgattgcag aaaggggcag
     3241 ggtggtcaat cagggagcgg gccgatgtaa ttcgcccgta caaggacgca acgactattc
     3301 ggtgggaatt tcatagtgga tgatgaattc gaccgattcc ggcagggtgt ccggggtgta
     3361 gccgatggtc gaggccacga cctcgcgggc gccggcctcg tcgaacccgc cgccggccac
     3421 ggcgatgatg gcgttgacga ccgactgcgt caccatctgg ccctggatga ccagcgtggc
     3481 gccttcgtcc gcggtgccga ccgagccggc cttgaccgtc agttcggcca gaaccgagtt
     3541 gtccgacttc gtgatgatgg gcaccagcag ggtttccgcc acaccgccaa agatcggatc
     3601 cgcgttgtcg gcaaggatcg agaacctgcc cgactcgaca tacccttcgt ggaaggcgct
     3661 gtatccgacc gcgacggtgt ccgcgtcgat gcgaccgaaa acgatgttgg gcatgacgga
     3721 cgtcacgatt tccatgccgg tcggcttcag gatttcgccg gtgaattcgg tcatcgtctt
     3781 cgattcgacg gcaacaacgt cggccgacca tgtgaattcg gtcccgtccc tgctgcccga
     3841 aacatgaatt ctgtatgtcc cggtcgtcgc ataggcgtgg gtgaaggaag tgccgttctg
     3901 gactgccgat ccgtcgccga aatcgatatc gacggcgccg accgccgcat cggtcacggc
     3961 gacctggaaa ttctggttga ccgtcaggaa cgcgtcctgc agcgacacgg cctggctggt
     4021 gctcaggatg acctcggccg ccagttcgtc gatgcgggtc cagtagtaag cgttgtgcgt
     4081 gcggcagtgg acgcggaagc cgtaagtcgt ccagttttcg tcctggtcgc agttttcccc
     4141 gccgccgatt gaacgagcaa gcggctggta cctgaactgc tgctcgcggg cggcgatcac
     4201 cgcctttgcg tccgcggtga cctgtatggc ggcttcaagg tcggccctgg cggccgtctg
     4261 catcgcttcg tcaaggttca actgcgcaag gcggaacttc acgacggcgc cgtaaaccag
     4321 caactggtgc ttcgcgcgca ggccggtgac cttgattccg tcaaggatct cgtcgaagaa
     4381 gggcgccgcc ggcccgggca cgtccgcccg aaccgcttcg agccttgcga ccagcgcttc
     4441 atagccggca accatggttg ccagttcggc agcctcgacc gcctgccacg attcggcttc
     4501 ttccttcgtc cagttcatga tcgccttcgg tggcggcgga agttcgtgga aaacgacccc
     4561 aatcgaagcg gcggcctcgg tttccgggtc ggtcccgacc aggtaacgca ggatgtggcc
     4621 atagatcatc tcgttttcct gcatggccac gacgtcttcc atgacggccc ggacttcgcc
     4681 cgcggccgtg cccatcggca tcgtgatgtc ttcaaggcag tcggtccagc ggtagtcggt
     4741 gtcagcggac atcctgaaac tgcagtattc gttctgccag tagccccatt cgtggccggt
     4801 cccgaacacg tggtggccga ccagcttgcc cgacagcatg tgcttcagct tctggatgtc
     4861 gcggtcgcgc gcctcgatcg tgatcggcag gtacagcgga agctggttgt cgaaagtcag
     4921 ccaccaggct gactcgggga agtaccacaa ctgccgcttc tggtattcct gctccatgaa
     4981 gtcatacagg aaatggaagt cggtgttccc ataaacgggg gcaggtcttt ccaggtcata
     5041 gaacatcaag gtgtggcact ttacgccaat cgattccggg gcgaactgcg gcaggttgaa
     5101 gtacctgacc ccgtaatggg cggtcggttc gccgtacgtg ccgtggttcg tcgtgacgat
     5161 ctggacgccc gggtagttct ggtcgatgaa gtcggcgatc accgtcatct ggtgaacaac
     5221 aagctggtcg tccatctcgg tgaattcggt cgggtcgaag ttgaagctga agaagtccgg
     5281 gtagttgtcc gggtcggtgc ccatcaattc cgtaatcttg tccgttatct gctggtcggc
     5341 cgggcgaggg tcgtccgggt cgatcagtcc gtggtttccc tgctgcatgc cgtaaaggcg
     5401 gaatccggcg ctggtcgtga acccgcgcat gatcccgtac tgttcgtacc cttcaaccgg
     5461 gatgccgccg cgaccgtcgg aagccaggtt cttcacgcac cagtcgatat agttgatgcc
     5521 gtcggaaaga tgctccgcct tgccaagcgt gaagatgtcg cccatctcaa gcgggtgggt
     5581 caggtgaagc gaaacgctgc ggtacttgaa cgccggcgtg aattcgcgga aggccggttc
     5641 tgccggcttt gccatttcgg ccggaacgta ctcctgttcg gggtggaaga acctgatgcc
     5701 aaggccgtgc agccagtcgt acgccgcgta ctggcgtcca agcagcccgc cgccggacag
     5761 ttcgatcagg atgccgctgc cgcaccacgt ttcctgaaag cggaaggtct ggtcggtcgg
     5821 cgcttccgtg tcgaaaaccg cttcaccgat gccatcgcca atgaatacga cgcgacccac
     5881 gccgtcgcgg catttcgtgc tgtcgttcgc gtcgtccagg accgcgctgc gtcccatcag
     5941 gccaaggtac ttgaccacgt cgtcggcggc cagcttcacc gcctcgggcg ccgtcggtga
     6001 aatcgccacg gtccactcga ccggcacggc gcattcgggg cagggcgcgg gcgtgctttc
     6061 ggcgcacccg gccgcaagaa tcagtgtcgc aatcgtcggt atcgcaattc ttgttcggca
     6121 gttcatgaaa cagaaccaca aaagagaccg cggcattgta ggcagaagct tgattgctgt
     6181 gtagtgtcaa agtgcgtcaa aaattcgctg gtgcattcaa cccgccgatg caaaacccga
     6241 accgggcgta tgtcaccttt tgattctgta cgggcgaatg aaaattcgcc cgtttgcgtt
     6301 aaaccccgtc cggcgtccct ggtggacttc agtcggtgat ggcgcagttg tggcccggtt
     6361 cggcgttgaa caggtcgctg ctggcctggc cgccttcttc gggagtgtcg ctgaccgtta
     6421 tcacggacgt cgaaatctgg cctgttacgt gtgtctcgtc cggcagtgtc gcaagcatgt
     6481 cgatcagtga aacaagcccg aagcccatca cctggacgtc gcaactgtac cttatgtcgg
     6541 caaagatcga ttcgtacgtg cccacgtcaa gggttcccgg acaggcaggc agcgaatcgg
     6601 caccgggggt gcatccttcg atgcggcccg cgcagtattc atcaaccagg atcctcgaca
     6661 ggtcggctat ggtcgggtcg cgcagtattg tcgcgtacac caggccgtcg gcgatcaccg
     6721 ggatgccgtt atcgtcgttc ctgatgcatc cttcgaaaac catgttcctg atgtccaggt
     6781 tggccttcca atccggattg accggatctt ccgacacgga cgaaaggaaa gatatcaact
     6841 gcgcctggat gttgaacggt cccgcttcga agcacggctc gccgcccttc cagtggatgc
     6901 ccatctcgac acatggggag cccgcgacgt acgtcaggaa gttgtcgatt tcccagcgag
     6961 aggcgatggt cattctgctg gcgtaaccgg tgccgtcttc gtatccgtca aacgaaatca
     7021 cgaatggaat ctgggccagc ccctcgccaa gcatctcgcc aaggccttcg gcgtcggaca
     7081 cgccgttcgt gacgtccagt ttctgaaccg ttccgattgc cagttcgtgg tcggcaatcg
     7141 tgctttcgcc aggttcgcat gtgaattcgg gcatttccgg gaagtcgata tattcctggt
     7201 tccggatggt cacggtgtcc gttccggcgt cgtattcgat ttcataccag aggttaacct
     7261 ggctgtggta atcctcgaca acgtagtacg gcgcgccgca gtaatcgccc ctccagaatc
     7321 cgtgggtgtg cccggtgaat atggacttga ccacccccgg gtattcctcg acgaccgagc
     7381 agagctgacc gccgggagcg ttgggtgaca gcgaactcgg cggatggtgg aagaacagga
     7441 aagaaggctt gccctgggca agttcggcac gcagccagtc gagctgggtc tgggagaatt
     7501 ttcccatcag cccgtgcgtc tcgccccacg aaccgtcttc catcgagtcc agcacgatca
     7561 gcttgacccc gttgatttcg gtcgtgaagt aaagcgggtg tcccattacc gtcgagaatg
     7621 cctgaccgcg cctggcgccg tcggtcgtca gcgtcggaac gtccccgaaa tagtcgtaaa
     7681 attcgtggtt tccaattgcc gcgacccagg gcatcgtcaa cttgtccatt tcctcgacga
     7741 accatgccat cgggctttca agcggttttt cgtcccacgc gggaagaacg tggtcgaaat
     7801 tgtcgccgcc cgtgaccagg atgtcatagt cgcccgaaag cgcattgatc atgggaatca
     7861 ggtcgccgat atccttcgaa tgtttttcct gtccttcgac ggttctgaag tggatgtcgg
     7921 aaacgaagac gaatcttgtg acggcgggcg gaatcacggc gtcggttgcc gcgtcgcccg
     7981 tcgcatcagt ctgatgcgca acatcgccgg cgacgtcttc cggcgccgtg gagtcgtcgg
     8041 caacgtcttc cggcatcgtc gaatcatccg tggtgtcgct tactggcgtt tcggaatccc
     8101 cgcatcccat gacgcccgat gtggccatgg cggctgcgaa tgcaatcaaa gtcactgaag
     8161 ataaaagtct tttcattgtc tgcccccaaa attgccggaa tcaggttagt cgcctgtcac
     8221 agggaaaagc gaccgcgcgg atgatagcag gattgacgcg tcgcatgaaa cagcttcttg
     8281 cgccgtcgtc gtcgcccgcc gattatcttg agtgatttca ggcctgaaaa tccttgtccc
     8341 gtctcgacaa agcgaccctt tagtggtaaa agggccgccg gttttccttg atagtgcaag
     8401 gactggtaaa tccatggaag aactgaaaaa tgaagcgctt gcgcagatag cccaggcgac
     8461 gacgacggcg gccctgaagg aagtcgaggt gcattggctc ggcaagaagg gcttgatcac
     8521 cgaacgactg aagacccttg gaaccctggc gccggaagaa cgcaagaatg ccggccgcga
     8581 cctgaatatc ctgaagaacg agatccagac ggcaatcgcc gagcgcacgg tcaccgttgc
     8641 ggccagcgaa atccagcagc aggccgcgtc gcttttcatc gatacgtcca tgccgggcat
     8701 cagggcccga tccggccacc tgcacccgct gacgatggtg accgagcagg ttgtcgatgt
     8761 cttcgagagg atgggtttct tcaccctgga ttatcccgag gccgagagtg aattcttcaa
     8821 tttcgacggc ctgaatatcc cgtcggatca tccggcgcgc gacacccagg acactttcta
     8881 cctgacggac ggcaacctgc tgcggacgca cacttcaccc ggccaggttc gggcgatgcg
     8941 cgaatttgcg ccgccattca aggccatctt ccccggccgc gtattccgtt gcgaggcgac
     9001 cgacgcgtgt cacgagcaca ctttcaacca ggtcgaaggc atcatgatcg accgttcggt
     9061 ttcggttgcc aacctgattt cgtcaatgat gacgctgctt tccggcgtgt tcggccgcca
     9121 ggtggtggtc cgtctgcggc ccggtttctt cccgttcgtc gaaccgggct tcgaactgga
     9181 tatccagtgc atggtctgcg ggggttccgg ttgtccggtc tgcaagcgca gcggctgggt
     9241 tgaactgatg ccatgtggcc tgatccatcc caacgtcatc agggcgggcg gccttgatcc
     9301 ggccgaatgg gaaggctggg cgttcgggct tggtctttcg cgccttgtca tgatgcgtta
     9361 cgagattgac gacatccgct ggctgatgtc gggcgatctg cgtttcgtgc ggcagttctg
     9421 aacgattccg ggccaccggc ggcccgtggg gactgaatat gaagtattcc tggaactggc
     9481 tttccgatta tgtcgatttg tccggcatcg atccgatgac cgttgccggc aggttcacca
     9541 tgaccgtcgc cgaactcgaa ggcgtcgaac aggtcggtga aggtcttgaa ggcgttgtcg
     9601 tcggccgtat cgttaatgtc gcggatcacc ccgattcggc caagctgaag ctgatcgatg
     9661 ttgacgtcgg ggccgccgga ggcgtggtct cgggcgtttc cggggcgccg aacgtcaagg
     9721 ccggcatcct ggtgccgatt gcccttcctg gtgttgtcct tccaggcgga gtcgaggtca
     9781 gggcggttga agtccggggc gtcgcgagtg ccctggtcgc cctgtccgaa aaggaaatgg
     9841 gcatttcgga cgatcacggc ggcatcatgg agcttccggc cgacagcgtg ccgggtgcca
     9901 ggctggttga caccattccc gtcagggaca ccgttttcga agtcgataac aagtcgatca
     9961 cacaccgccc cgacctgtgg ggacactacg gcgttgccag ggaagtggcc gccatgactg
    10021 gccgtaccct gaagccgctt gacgagacga tcccccaggg ccaggcggat ctgatgacag
    10081 tgcgtgtcga tgagccgatt gattgtccca ggtacatggg catgtgcttc gacggcgtcg
    10141 ggattgcacg gtcgccattc tggatccggc atcggctgcg ggcggtcgga catcgtccga
    10201 tcaacaacgt cgtcgatctt accaattaca tcatgtgcgc cgtcggcgag ccgatgcacg
    10261 catttgaccg ccggaacgtc ggcggcgaca ccatcatcgt ccgtcgcgcg gccgagggcg
    10321 aacctttcag gacgctggac ggtcaggatc acgtcctgtc ggccgaggat ctgctgattg
    10381 cggacgcttc gcgcggcgtg gcgcttgccg gtgtcatggg cggggaaaac agcgaagtcc
    10441 ttgacgatac gtcaacgctt ttcatcgaat gcgcgacttt ccatccaggc agggttcgca
    10501 ggactt
//
LOCUS       DAOPPA010000047        10438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7265_length_10438_cov_3.981219, whole genome shotgun sequence.
ACCESSION   DAOPPA010000047 DAOPPA010000000
VERSION     DAOPPA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7265_length_10438_cov_3.981219"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PKH54_02475"
     CDS             complement(<1..936)
                     /locus_tag="PKH54_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98781.1"
                     /translation="MNVRDLLSFCLVVALCASLPATVLAQDSGRKTIVLIFPIKVTGL
                     TITDGQRAQLKDYLDTRLTMEGVYKVMPESQVKRDLGKAKLDSYNDCYDESCRIDLTK
                     TVYADRCVSVEVLAESKSCRVTAKLFDIAQEATETAADVPTTCDFDAIKLAMVATAQQ
                     LSGKSAPAVVKQSAGGDSVFSVSALPSVPQVRQVGQVQAETAAGSWNDIDVAAYEAYD
                     AVLAKDGSGSVSPEDKVKAWEQFGGRYPGFAAQASKRAGEWRTFITQREEARRIETIR
                     RQRMEDDWSKLGRLLKLKVFTEERKQAWARAFVDAY"
     gene            complement(973..1254)
                     /locus_tag="PKH54_02480"
     CDS             complement(973..1254)
                     /locus_tag="PKH54_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98782.1"
                     /translation="MKVTRRRRRINIVSRRQDRDCFQMEGQGMFPDGMTGIVSGLLPG
                     GGFPMPRQVLSKDYGQRHAPSGGTFSFFDSGDQTLVKSGLLCDTNQHDD"
     gene            1280..2713
                     /locus_tag="PKH54_02485"
     CDS             1280..2713
                     /locus_tag="PKH54_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98783.1"
                     /translation="MTTPATIDFFGILKYAGRFPEMRMIGSRPIDIETLDRRSDSTDG
                     PINAFGGISPGMTFGDGWRVGGISVRQQEIDLQCEGPQGAATFMLGAPNQGPAWPFDT
                     ARARIGYHPSDLDETTLNRVGRDLADMIAGLPEMEGGFSDLVQKALDSRHVSGARPNP
                     AIVERRPDGKVYVRLTTRCQERCVFCFFFDSPAESWLDDAEGLPDEPERSLALLDPKA
                     VTQLIITGGEPTLADRFEDILSGLLERGFDNIVVQTNGIFTAKAGFLDRFKDFRKRVS
                     FGFSLHAADWRTSAAITGGGTEATFQAKLDSIQRAVELGYVTKITCVLTTINLDSLDD
                     MVGFCVGLKQVDPTLENVILQFSMPSVQGLMRSNRDLYPTLTAVADAVGPALDLADAG
                     GLRASFSSLCSVPPCCIPSHLRHLETMWYKKGPTDWWESERAYAPQCAECVLKPWCSG
                     VSPQYLEWFSDHELVPFAASDTTLLMP"
     gene            2769..4112
                     /locus_tag="PKH54_02490"
     CDS             2769..4112
                     /locus_tag="PKH54_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC98784.1"
                     /translation="MTRRFRHPAHLLLAAAFLLAGCGQPDGRTSRDESAQPGRNSDAT
                     TAPEKAQTTIVLAHPYIGAAAEAIAGAAAMFDESRPDISVQLRPTAVEDILFSIPRDI
                     LAGKGPDVFIAPDTFARHWATLDNILQPLDVLLKTTDGVQGNQPASGQTGAGASTGSR
                     LSPDGRPHGLPIAVRVPLLVANDDFLGTGFKFTPGDGSRPTAVSVVANDWLALTGFAT
                     AGGGHVIDGTGRAAFDDPAAATGMVALAGLLESGAIRPERSRQAIIDAFNDSGIAAMI
                     TWPDSISLISSSISWRIVPFPEIEGVGRPQPWWQMDSIFVSSWSRHRRQAADLAAWLA
                     SPAAADFLMRHDFPGLADGEGRMINPTEDDQAAFQYAALPVNRGILTQRAAAIPSPDL
                     PATATAATSFARLATDLAASPANAAKGAGAAVEKAARDARDAVTKVMQSSSFRLK"
     gene            4157..6154
                     /locus_tag="PKH54_02495"
     CDS             4157..6154
                     /locus_tag="PKH54_02495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98785.1"
                     /translation="MPDDVFEKYELVRRLGSGGMGEILLARQKGAAGADRNVIIKMIL
                     PHLAQDPEFIGRFTDETRIAALLSHGNIVTIFESGCWQGRFFMVMEYVDGPDLKELLS
                     MAGPLRQSDRWVPFCVHVLSETARALAYAHSKRDQEGRPLGIVHRDVSPSNILISREG
                     LVKLTDFGVAKAARRTSVTLPGKLHGKVNYMAPEQVRGMDCDHRSDIFSLGVTGYEML
                     AGVRPFEGDSDIAVLEQIRSMRFVPLDQAAPSVPPALAAIVARAMDADPAARWQSADE
                     FLAALGAFAHQAGMSMMARDVAAMLAPLLTTTGDEVRANASIDDFLLDALNDLPGTPS
                     GGIRISTGEVGGGSRERTVTVSREVTSPGSARGAARDATAAAGFAEDGAAPDQSADGR
                     TPERDAGLQSATGSDRRTSRGRAGLIAAGVAVVLLAAGYATWTAIERTPDVDGRPQTG
                     AASAGRTDTSGTITPTPPDPAPTATAGQAKSQVGAAAIVPPSPTDSPSSKAGTPEPAT
                     AIDSNPGGARQVREGDKTVEASALTNATDASTGDMAVTRQTGRQNEKTPQLPGDAQRP
                     QTTRTAAVTRTGPSDSSARADTGRAVFRFFPADAVVEIDGRRVATDGNLVELDLPAGS
                     HTIAVSGPDGAHKRTRSFNIVAGGTLALGTIELESGTDSGR"
     gene            6215..7582
                     /locus_tag="PKH54_02500"
     CDS             6215..7582
                     /locus_tag="PKH54_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC98786.1"
                     /translation="MASETRTVILDVPVEDVTLNKFLLKVVDGPDRGQSRTFQKRQVT
                     IGTDRSCDFQLSDPTVSRIHAMIEFGPAGFRLKDDGSKNGVFAGDLRVFEAILTDGIE
                     FLVGKTRIAFRRSTETVSIQIATAGRFGSMLGESIEMREVFAIIKKAAPTDTNVLIQG
                     ESGTGKELVAQALHQSSNRARGPFVVFDCSAVPAELIESELFGHVKGAFTGAVRDRVG
                     AIGEAEGGTLFLDEIGELPIDLQPKLLRVLESREVRQVGGTGTRKVNFRLVAATNRFL
                     EQETAAGTFRADLFYRLSVITISLPALRRRPQDIPALVNHFLDEIATREGGARVRLSW
                     ETMERLKLHPWPGNVRELKNFVERSVILSGALAGTPRALDLPPPLTGPVNVGGPDGIA
                     LDLDAPFKQEKDRIVDEFQRRYFTRLLRQTDGNISKTARLAGIHRKTLEYLLKNLDID
                     WQAEG"
     gene            7707..7994
                     /locus_tag="PKH54_02505"
     CDS             7707..7994
                     /locus_tag="PKH54_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98787.1"
                     /translation="MKKQTVAVGVKTDTITRADVRNAINAGVLTIEEERYIRLRYGIS
                     EGPTATIVRRGQDNPTTRATLAMIEASMVPEQLTQVNQAVKSRIINRLRNQ"
     gene            complement(8016..8708)
                     /locus_tag="PKH54_02510"
     CDS             complement(8016..8708)
                     /locus_tag="PKH54_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOC98788.1"
                     /translation="MIPSVTLAEIERLITRAAGRMYDRGLVSGHWGNITAITPDGSLL
                     GTPQGLCLKDVTAGDLVQMDRNGVKIGGRGRPFFEIGMHIAVYHERPDIGAVVHAHPP
                     AATTFACAGLPIDGRFSPEFTFMTGGVVPVVPFAMPGSRTLDNNLAPFLEKHDVVMLE
                     NHGILAWGPCPVTALMLIEQVEEIARIMLNSNQVGKISPLPEEVVGLIMDARTRAGRG
                     PAGRERKTVRGR"
     gene            complement(8705..8989)
                     /locus_tag="PKH54_02515"
     CDS             complement(8705..8989)
                     /locus_tag="PKH54_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC98789.1"
                     /translation="MILGRIAGTVVSTVKHPEYVGHKLLVVQPIDPTGRPNGESFLAV
                     DDVQAGVGDTVLVIREGNGARQIFKRRDFPIRAVIVGIVDDVNIPEGDHR"
     gene            complement(8986..9261)
                     /locus_tag="PKH54_02520"
     CDS             complement(8986..9261)
                     /locus_tag="PKH54_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC98790.1"
                     /translation="MYLGRVIGTVVASRKAAGLNGIRLMAVIPINHDGSDRGQMIVAA
                     DAVNSGPGDIVHLVGSREASLAFSDQFVPLDAAIVGIVDQIHIPEEP"
     gene            complement(9393..9554)
                     /locus_tag="PKH54_02525"
     CDS             complement(9393..9554)
                     /locus_tag="PKH54_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOC98791.1"
                     /translation="MKYICTVCNWIYDPAIGDPDGGVAPGTKFEDIPDDWTCPECGVT
                     KEDFEPLDK"
     gene            complement(9795..>10438)
                     /locus_tag="PKH54_02530"
     CDS             complement(9795..>10438)
                     /locus_tag="PKH54_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98792.1"
                     /translation="MDTLEMGMVESGGRLFPFGGLGVDAQILADYLSVKQGIGAGVLK
                     PIFQNVGGYFLAFFGVTAPRTIANLLAGREAIVRVINIDQPAYRILDDGSAGQEYAPG
                     AVLYEGPFLMLMAGTCPYYGYGMKVLPFADLRPDAFNLRMVGISSAKALANLPTIWTG
                     DYRDPGILDFYCRSVRIELSDPSPCQQGGDLVGTPTRLHFRHVPRVVKLLRFI"
BASE COUNT         2094 a         3232 c         3207 g         1905 t
ORIGIN      
        1 ataagcatcg acgaatgccc ttgcccaggc ctgcttgcgt tcttcggtaa aaaccttcag
       61 tttcagaagc cggccgagtt tggaccagtc gtcttccatg cgctgacgac gtatcgtttc
      121 aatccgcctg gcctcttccc gctgggttat gaacgtccgc cactcgcccg cacgctttga
      181 agcctgggcc gcgaatcccg gataacgtcc cccgaactgt tcccaagcct tgaccttgtc
      241 ttccgggctg acggaaccgg aaccatcctt tgcaagaacc gcgtcgtacg cctcgtatgc
      301 ggccacgtcg atgtcgttcc aggaaccggc cgcagtctcg gcctggacct ggccgacctg
      361 ccgaacctgc ggaacggaag gaagcgccga aaccgaaaag acgctgtccc caccggccga
      421 ttgcttgaca acggcaggcg cggacttgcc gcttaactgc tgggcggtcg ccaccatggc
      481 aagcttgatc gcgtcgaaat cgcaggttgt cggaacgtcg gccgcggtct cggtcgcttc
      541 ctgggcgata tcgaaaagct tggccgtcac gcggcaggac tttgattccg caaggacttc
      601 caccgacacg caccggtcgg catagacggt tttggtaagg tcgatgcgac acgactcgtc
      661 gtaacagtcg ttgtacgaat caagcttcgc cttcccaaga tcgcgcttaa cctgtgattc
      721 cggcatcacc ttgtaaacgc cttccatggt caggcgggta tcaaggtaat ccttcaactg
      781 ggcacgctga ccgtcggtaa tggtcagccc ggtaaccttg atcgggaaaa tcaggacaat
      841 cgtctttcga cccgaatcct gggcaagaac cgttgccggg agtgatgcac agagggcaac
      901 aaccaggcaa aaagacaaaa ggtctcgaac attcatttgc gacaaccccc atcttccgaa
      961 acaggtcaga ctttaatcgt catgctggtt tgtatcacat aataggccgg atttcaccaa
     1021 ggtttgatcg ccgctatcga agaaagaaaa cgtcccgccc gaaggagcat gtcgttgacc
     1081 ataatctttt gacagaacct gtcgtggcat cggaaaaccg cccccgggaa gcaatcccga
     1141 aacaatccct gtcatgccat ctggaaacat tccctgtcct tccatctgga agcaatccct
     1201 gtcctgccgc cgggaaacga tattgattcg gcggcggcgg cgcgttacct tcatcgttaa
     1261 tcacgggatt gcgaaggcca tgacgacgcc agccaccatc gacttcttcg gcattctgaa
     1321 atacgcggga cggttcccgg aaatgcggat gatcggaagc aggccgatcg acatcgagac
     1381 gcttgatcgc aggtcggatt cgaccgacgg accgataaac gccttcggcg gcatttcgcc
     1441 cggcatgacg ttcggtgacg gctggcgcgt cggcggcatt tcggtccggc agcaggaaat
     1501 cgacttgcag tgcgaagggc cccagggcgc cgcgactttc atgcttggcg cgccgaacca
     1561 gggaccggca tggccgtttg acaccgcccg cgcaaggatc gggtaccacc cgtccgacct
     1621 ggacgaaacg accctgaacc gtgttggccg cgaccttgcc gacatgatcg cgggccttcc
     1681 cgaaatggaa ggcggctttt ccgacctggt acaaaaggcg cttgattcgc ggcacgtttc
     1741 gggcgcccga cccaacccgg ccatcgtgga acggcggcca gacggcaagg tctatgtcag
     1801 gctgacgaca aggtgccagg aacgctgcgt tttctgcttc tttttcgaca gtccggccga
     1861 atcgtggctt gacgacgcgg aaggcttgcc ggacgagccg gaacgaagcc tggcactgct
     1921 tgatccgaaa gccgtgaccc aactgatcat caccggcggc gaaccgacgc ttgccgacag
     1981 gttcgaagac atcctttccg gtctgcttga aaggggtttt gacaacatcg ttgtccagac
     2041 caacggcatt ttcacggcaa aggccggttt ccttgatcgt ttcaaggatt tccgcaaacg
     2101 cgtttccttc gggttttcac tgcacgcggc cgactggcgg accagcgccg caatcaccgg
     2161 aggcggcacg gaggcaactt tccaggccaa gctggattcg attcagcggg ctgtcgaact
     2221 tggatatgtc accaaaatca catgcgtcct gacgacgatc aacctggaca gtcttgatga
     2281 catggtcggg ttctgcgtcg gcttgaagca ggtcgatccg acgcttgaaa acgtcatcct
     2341 gcaattttcc atgccgtccg tgcagggtct tatgcggtca aaccgcgacc tttatccaac
     2401 acttacggcg gttgccgatg ccgtcggccc ggcccttgat ctggccgatg ccggcggtct
     2461 gcgtgcaagt ttcagttcgc tttgctcggt gccgccatgc tgcattccat cacacctgcg
     2521 ccacctggaa acgatgtggt acaaaaaggg gccgaccgac tggtgggaaa gcgaacgggc
     2581 atacgcaccg cagtgcgccg aatgcgtcct gaagccatgg tgttccggtg taagccccca
     2641 atacctggaa tggttttccg accatgaact ggttccattt gccgcttcgg acacgacctt
     2701 gctgatgccg tgacataaga caccgcaaca ggttaaaatg acgaccggtt caaaacggaa
     2761 accggctgat gacacgtcgc ttccgacatc ctgcgcatct tttgcttgcg gcggcgttcc
     2821 tgctggccgg gtgcgggcag ccggacggaa ggacatcaag ggatgaatcg gcccagcccg
     2881 gccgcaattc cgatgcgacc acagcacccg aaaaggcgca gacgacaatc gtcctggcgc
     2941 acccttacat cggcgcggcg gccgaggcta tcgcgggcgc tgccgcaatg tttgacgaat
     3001 ccaggcccga tatttccgtc cagttgcggc cgaccgccgt cgaggacatc cttttttcga
     3061 tcccgcgcga tatcctggcc ggaaagggcc cggacgtttt catcgcgccg gacacgttcg
     3121 cacgccactg ggcgactttg gacaacatcc tgcaaccgct tgacgtgctt ttgaaaacga
     3181 ccgatggcgt ccagggcaac cagccggctt ccggacagac cggggccggg gcatcaaccg
     3241 ggtcacgcct ttcgcccgac ggacgtccgc acggcctgcc gattgccgtg cgcgttcccc
     3301 tgcttgtcgc caacgacgac ttccttggta ccggattcaa gttcacgcct ggcgacggtt
     3361 caaggccgac cgccgtttcc gtcgtcgcaa acgactggct tgcactgaca ggattcgcga
     3421 cggccggcgg cgggcacgtg attgacggca ccggtcgggc cgcgttcgac gatcccgcgg
     3481 ccgcaaccgg catggtcgcg ctggccggcc tgctggaatc gggcgcaatc cggcccgaac
     3541 gttcacgcca ggcaatcatc gatgccttca acgattccgg aattgccgcg atgatcacgt
     3601 ggccggattc catttccctg atttcatcgt cgatatcgtg gcgcatcgtt ccattcccgg
     3661 aaatcgaggg tgttgggcga ccgcagccat ggtggcagat ggactcgatt ttcgtaagtt
     3721 cgtggtcgcg ccaccggcgc caggccgccg accttgcggc gtggcttgcg tcgcccgcgg
     3781 cggccgattt cctgatgcgg cacgacttcc cgggacttgc ggacggggaa ggcaggatga
     3841 tcaatcccac tgaagacgac caggcagcgt ttcagtacgc ggccctgccg gtcaaccgag
     3901 gcatcctgac ccagcgggcg gcggcgattc cgtcaccgga tcttccggcc accgcgaccg
     3961 cggcgacgtc cttcgcccgg ctggcaaccg accttgcggc ttcaccggcg aacgcggcga
     4021 aaggcgccgg ggcggctgtc gaaaaggccg cccgggacgc acgggatgct gttacaaaag
     4081 tgatgcagag ttcttcgttc cgcctgaagt aaaataccgc ggtttgtttt attcgtgatg
     4141 acctggtgtt tcgaccatgc ctgatgacgt gttcgaaaaa tacgagcttg tccgacgcct
     4201 gggttctggc ggaatgggcg agatcctgct tgcccgccag aagggcgcgg ccggggcgga
     4261 tcggaacgtc atcatcaaga tgatcctgcc ccatctggcg caggatcccg aattcatcgg
     4321 acggttcacg gacgaaacca ggatcgccgc gcttctttcg cacggaaaca ttgtcaccat
     4381 cttcgagtcc ggctgctggc agggcaggtt cttcatggtc atggaatacg tcgatggccc
     4441 agacctgaag gaactgcttt cgatggccgg accgttgcgc cagtccgatc ggtgggtgcc
     4501 cttctgcgtc cacgtccttt cggaaacggc gcgtgcactt gcgtacgcac acagcaagcg
     4561 cgaccaggaa ggacggccgc tgggaatcgt gcaccgcgac gtcagcccgt caaacatcct
     4621 gatttcacgc gaaggcctgg tgaagctgac cgatttcggc gtggcgaagg ccgccaggcg
     4681 gacgtcggtg acattgcccg gcaagttgca cggcaaggtc aattacatgg cgcccgagca
     4741 ggtgcgcggg atggattgcg accaccgatc cgacatattc agccttggcg tcaccggtta
     4801 cgaaatgctg gccggcgtcc gcccgttcga gggcgactcc gacatcgccg tgctggaaca
     4861 gatcaggtcg atgagattcg tgccgcttga ccaggccgca ccgtccgttc cgccggcgct
     4921 tgcggcaatc gtcgcgcgcg ccatggatgc ggatccggcg gccaggtggc agtccgccga
     4981 cgagttcctt gcagcccttg gtgcctttgc ccaccaggcc ggcatgtcga tgatggcccg
     5041 cgatgtggcc gcgatgctgg ccccgctgtt gacgacgacc ggcgatgaag tccgcgcgaa
     5101 cgcgtcgatt gacgacttcc ttctggatgc gttgaacgac cttcccggca cgccttccgg
     5161 cggaatcagg atttcgacgg gcgaagtcgg cggcgggtcc cgtgaaagga ccgtcaccgt
     5221 aagccgggaa gtgacatcgc ccggttcggc caggggcgcg gcccgtgacg cgaccgcggc
     5281 ggccggtttc gcagaagatg gcgcggcgcc ggaccagtcg gcggacggac gaacgccgga
     5341 acgggacgca ggccttcaat ccgcgacggg ttccgatcgg cggacatcac gcggacgcgc
     5401 cggcctgatt gcggccggcg tggcggtcgt cctgcttgcg gcaggctatg cgacatggac
     5461 ggccattgaa cggaccccgg acgttgacgg tcggccccag actggggcgg cgtctgccgg
     5521 acgaacggat acgtccggca cgattacccc gacgccaccg gatcccgcgc cgaccgcaac
     5581 cgccggccag gcgaagtccc aggttggagc cgcagcgatt gtgccaccct ccccgaccga
     5641 ttccccttcg tcaaaggcgg gtaccccgga accggcaacg gcaattgaca gtaatccggg
     5701 cggcgccagg caggttcgcg aaggcgacaa gacggtggaa gcctccgcgc tcacgaacgc
     5761 gaccgacgcc tccacgggcg acatggctgt cacgcggcag accggcaggc agaacgaaaa
     5821 gacgccgcaa ttacccggcg atgcgcaaag gccccagacg acaaggacag ccgccgtcac
     5881 ccgcacaggc ccttccgatt cctcggcgcg ggccgacacg ggcagggccg tcttccggtt
     5941 cttcccggcc gacgccgtcg tcgaaatcga cggacgccgc gtggcgacgg acggcaacct
     6001 ggtcgaactc gatctgcccg ccggatccca cacgattgcg gtgtccgggc cggacggcgc
     6061 ccacaaaagg accagatcgt ttaatatcgt cgcgggcggc acgcttgctc tgggcaccat
     6121 cgagctggaa tccggaaccg attccggacg atagcccgat tgcccgacca cggacggtca
     6181 acggatcgcc cttttcgcga tgcggggaaa cacgatggct tccgaaacca gaaccgtaat
     6241 tctggacgtc ccggtcgagg acgtaaccct taacaaattc ctgctgaagg tcgtcgatgg
     6301 cccggatcgc ggccagtccc gcacgttcca gaagcgccag gtcaccatcg gcaccgaccg
     6361 ttcatgcgac ttccagcttt ccgacccgac cgtttccagg attcacgcga tgatcgagtt
     6421 cggccccgcc ggcttcaggc tgaaggacga cggcagcaaa aacggcgtgt tcgccggtga
     6481 tctgcgcgta ttcgaggcca tcctgactga cgggattgag ttcctggtcg gcaagacgcg
     6541 gattgcattc cgccgaagca cggaaacggt cagcatccag attgcgacgg ccgggcgctt
     6601 cggaagcatg cttggcgaat cgatcgaaat gcgcgaagtc ttcgcgatca tcaagaaagc
     6661 ggccccgacc gacacgaacg tcctgatcca gggtgaaagc ggaaccggaa aggaactggt
     6721 ggcgcaggcg ctgcaccagt caagcaaccg cgcgcgcggc ccgttcgtgg tgttcgactg
     6781 ctcggctgtc ccagccgaac tgatcgaatc cgaactgttc gggcacgtaa agggcgcctt
     6841 caccggggcg gtgcgcgacc gcgtcggggc gattggcgaa gccgagggcg gcacgctgtt
     6901 tctggacgaa atcggggaac ttcccatcga ccttcagcca aagctgctgc gcgtgcttga
     6961 atcgcgcgag gtgcgccagg ttggcggcac cggcacgcga aaggtcaatt tcaggcttgt
     7021 cgccgcgacc aaccgcttcc tggaacagga aaccgccgcc ggcacgttca gggccgacct
     7081 gttttaccgg ctgtcggtaa tcaccataag cctgccggcc cttcgccgaa ggccccagga
     7141 catcccggcg ctggtgaacc atttcctgga tgaaatcgcg acccgggaag gtggagcacg
     7201 cgtcaggctg tcgtgggaaa cgatggaaag gctgaagctg cacccgtggc cgggcaacgt
     7261 tcgcgaattg aagaacttcg tcgaaaggtc ggtcatcctg tccggggccc ttgccggaac
     7321 gcccagggcg cttgatcttc cgccaccgct taccggcccg gtgaacgtcg gcggcccgga
     7381 cggaatcgcg cttgacctgg acgccccgtt caagcaggaa aaggatcgga tcgtggatga
     7441 attccagcgg cgatacttca caaggctgct gcgccagacg gacggcaaca tatcgaagac
     7501 ggccaggctt gccggcatcc atcggaagac cctggaatac ctgctgaaaa acctggatat
     7561 cgactggcag gccgaaggtt gatctttttg tctggcgtta ttgaaaaatt gtggttaatt
     7621 tcgtgctgca cgaatttggc aggatttgtg aaccggggtt atattggaaa gtcccgggtg
     7681 gtccggggtt ttgctggggg tcaatcttga agaagcagac tgttgcagtt ggtgtcaaga
     7741 ccgacaccat cacaagggca gacgttcgca acgcaattaa tgccggtgtc cttaccatcg
     7801 aggaagaacg ctacatccgg cttcgatacg ggatttccga aggtccgacg gcaaccatag
     7861 tgcggcgagg ccaggacaat ccgaccacgc gggcgacgct tgcgatgatc gaggcttcga
     7921 tggttccgga acagttgacg caggtcaacc aggcggtcaa aagccgcatc atcaaccgcc
     7981 tgcgcaacca gtgatgggat ttcgggcccg acctgctatc tgcccctgac cgttttccgt
     8041 tcccgcccgg cgggaccgcg accggcccgc gtgcgggcat ccattatcag gccaaccacc
     8101 tcttcgggaa gcggcgatat ctttccgacc tgattggaat tcaacattat ccgggcaatt
     8161 tcctcgacct gctcgatcag catcagggcg gtgaccgggc acggacccca ggccaggatg
     8221 ccgtggtttt caagcatcac cacgtcatgc ttttcaagga aaggtgccag gttgttgtcc
     8281 agggtgcggc tgccgggcat cgcgaacggg acgaccggca cgaccccgcc ggtcatgaag
     8341 gtgaactcgg gggaaaagcg gccgtcaatc ggcaggccgg cgcatgcgaa cgtggtcgcg
     8401 gccggggggt gcgcgtgaac caccgcgccg atgtcgggac gctcgtgata aacggcgatg
     8461 tgcatgccga tttcgaagaa cgggcggccg cggccaccga tcttgacgcc gttgcgatcc
     8521 atctggacca ggtcgccggc ggtcacgtcc ttcaggcaaa ggccctgggg cgtgccaagc
     8581 agtgaaccgt ccggcgtgat ggccgtgata ttgccccagt ggccggaaac caggccgcga
     8641 tcgtacatgc ggccggcggc cctggtgatc agtcgttcaa tttcggcaag cgtcacggac
     8701 ggaatcatct gtggtcacct tcgggaatgt tgacgtcatc gacaatgccg acaattacgg
     8761 cccttatcgg gaaatcacgg cgcttgaaaa tctggcgggc gccgttgcct tcgcggatga
     8821 caaggacggt gtcgccgacg ccggcctgga cgtcgtcaac ggccaggaac gattcaccgt
     8881 tcggccgccc ggtcggatcg atcggctgca cgacaagcaa cttgtgcccg acgtactccg
     8941 ggtgcttgac tgtggaaacg accgttccgg cgatgcgtcc caggatcacg gttcctccgg
     9001 gatatgaatc tgatcgacga ttccgacgat ggcagcgtca agggggacga actgatcgga
     9061 aaacgcaagc gacgcctccc ggctgcccac caggtggacg atgtcgcccg ggccggaatt
     9121 gaccgcgtcc gcggcgacaa tcatctggcc gcgatcactg ccgtcgtggt ttatcggaat
     9181 aacggccatc agcctgattc cgttaaggcc ggcagccttg cgcgacgcga cgacggttcc
     9241 gattaccctg ccaagataca tcaacagcct tgcagttcag ccccgccggt tgtaccgaca
     9301 aaagggcggc gtgtcaatcg atccgcatcg gacggccggc atgcgccgga tcacgggccg
     9361 gacaggcagc cgccccgacg gatatcgggt tgttacttgt caagcggctc gaagtcttcc
     9421 ttcgtgacgc cgcattccgg acaggtccag tcgtccggga tatcttcaaa cttcgtcccg
     9481 ggggcgacgc cgccgtccgg atcaccgatc gccgggtcat aaatccagtt gcatacggtg
     9541 cagatatact tcatgacgaa acctccatga tgacatcaac ctggtggatg cgacgccacg
     9601 cacaccacac gtcttcgaag ccagattctg ccacacagtc gcgcggcgcg gcagtgaaat
     9661 gtcgcaacga cttgcgatga aatgcgaaat ggggccgcgc ggcatcgaga tgacgcccac
     9721 gggaacccgg gaagacccga cgggggcagc atcgtggccg cggaacgaac tgccggtgcg
     9781 acagggttgt tgcttcagat gaagcgcagc agtttcacga cccgcggaac atgcctgaaa
     9841 tgcagtcggg tcggcgtgcc gacaaggtcg ccgccctgct ggcaggggct tgggtcggaa
     9901 agttcaatcc gaactgaacg acaataaaaa tcaaggatgc cggggtcgcg gtaatcgccg
     9961 gtccagatgg ttggcaggtt cgccagtgcc ttggccgaac tgattccgac catgcgcagg
    10021 ttgaaggcgt ccgggcgaag gtcggcaaac ggaagaacct tcatgccgta accgtagtat
    10081 ggacatgtac cggccatcag catcaggaaa gggccttcgt acaggacggc gccgggcgca
    10141 tactcctgac cggcggaacc gtcgtcaagt atccgatatg ccggctgatc gatgttgata
    10201 accctgacaa tcgcctccct gccggcaagc aggttggcga tggtgcgggg ggccgttaca
    10261 ccgaagaatg caaggaaata tccgcccaca ttctggaaaa tcggcttcag gacgccggcg
    10321 ccgatgccct gcttgacgga aaggtaatcg gcaagaatct gggcatcaac gccaagcccg
    10381 ccgaacggaa aaagcctccc gccggactca accatcccca tttccagcgt atccatcg
//
LOCUS       DAOPPA010000048        10424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7282_length_10424_cov_4.212557, whole genome shotgun sequence.
ACCESSION   DAOPPA010000048 DAOPPA010000000
VERSION     DAOPPA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_10424_cov_4.212557"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKH54_02535"
     CDS             complement(<1..1539)
                     /locus_tag="PKH54_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98793.1"
                     /translation="MNPVRTLEVLLAAMFICVSARAASPESEIVIARDPAWQGLIAGA
                     VKGRGGIDAAVARLDAIRSGGSECEPGRVPGGVIDWVVAGLYEQAGRIDAAIARYRAA
                     AGSPLGDVASFRIGVLLDQKNDIAGARAAWGAVSPASAMWVEARRKLAGSLMAENRPE
                     AAVQVILELFHDEMRPHEILSARLALARALDAAGRRGEAVAMALAAWFLDRDGSHSRD
                     AAALLKSLGSPLSPAIEVIREIESSRRRDLKPLCRRIARNRKALSSIDPALPEAASMA
                     CLIYRDRIADSTAGDLQVALGRASSPDVRAWIIKLAAEAAMDGGDDAAAGEWLARLVD
                     GYPASPLAPGAASDGARAFMRVGLHDRASEILEAASKSVMAPALARNSDWMKALAAMM
                     AGRREQALAHLDAILETRDRGNGVMFGLAERARYFRGVTLFGMGRSVEAREDLERVAR
                     ASCCSWYGMLAASRLSGWFGVQVDGGAPAGLPDGTHARDLAGSVGHGLPAVIRTGATS
                     APGVF"
     gene            1661..2296
                     /locus_tag="PKH54_02540"
     CDS             1661..2296
                     /locus_tag="PKH54_02540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOC98794.1"
                     /translation="MLQDALTGGRQAAALAALFHASLIEYVFPVFPGDTVTIAGAVLA
                     VTSGLPLWGVFAATMAGGLAGSALDFWIGRAFGRGWKTAGPEFKRRHPWLDRVLGQAD
                     RLSTRFARHGTVLLLANRMMPGIRSLLFVAAGMAGMSFVKSMLAGLVSNAAWTGLLML
                     AGLALGANLDSVVMLFRTYSVAVWCVIAAALGLWLTAWIRRRRTDRREAGP"
     gene            complement(2256..5030)
                     /locus_tag="PKH54_02545"
     CDS             complement(2256..5030)
                     /locus_tag="PKH54_02545"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOC98795.1"
                     /translation="MAGSSGIAMKSDDKTTPVLSGRQQPPVLDGDTESDSSMVRRQGR
                     FMRWLSRTFFGAVRFDESNIDAIRIACSAGTPVFVMDAHSRLDYLYFNYAFARFGLPL
                     VFFANEISMSAFRPMSQYLRRVWMRLTGRGFPPLPDSEMMRAGLGAGRPCLIFLKRPK
                     ALSQWGAEYRISYLKDLVEIQSEVERPLMIIPLLLVWEQKPESYRRSVLDFVFGDPQA
                     PGALRKALSFIWNFRSAHAQVGSPIDLRSFVQESPDRDPDAVAVRLKFAIAETLLRES
                     RAIRGPVLKGRGVMVDEIMRTRPFKDAVTTAGAQVGLGPAASMKKAGAWLRNMAADFS
                     VGWLDAFGVVLAVIFQRRFTGISVDTAGLARIREVGKNAPVVLVPSRRSILDYFVYSF
                     VFYTHGLIPPHVAVREQRKLNFVKPVLRRTGAFFVRHRIGSNPVYERTLRQYVRKLVK
                     EGYWLEFFIEGSRSRSGKLQPPRGEILSMVVDAVASGAAPDVWLVPCSVTYERIVEGS
                     FFERERRGDKSGIGVVSLANIMKRARNLGSRFGQLYMKFGEPVSMTAWLGGQGVAIPL
                     EDGASVDPMLVRRLGYSLMWDINSRIVVTPQNLVAFVLMTTRARGIPRPVLVEQTVEL
                     YRRIAIRGGLFSDRLDAAMLPGDGKFSTGAWRRVYVDWRDTAEMQRPGLPTLEAPRIT
                     AIDGLIEEVLGVFAREGAVKVSGEGFDSVISVVERHRAWLEYYKNGIVHFFLPQAIVA
                     TAVESLAAADRMDEQELHRRTRDLTRLFRLEFVYDKTVSMLESRSRTVDFFVDEGLLV
                     RVSDGIQVNRMRMDEINWYSEILRPFVDSYRVLATVVMKAPARADRDEIIAQAMKDGR
                     RAWEIGEVDKVESVSPVIFKNAYKLMQDEVANGRQPDMKAAAVSAYNALPETRRRVKA
                     PLPVDPSSGA"
     gene            5114..7069
                     /gene="uvrC"
                     /locus_tag="PKH54_02550"
     CDS             5114..7069
                     /gene="uvrC"
                     /locus_tag="PKH54_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOC98796.1"
                     /translation="MGWSPEKAAAFTADLPHRPGVYIMRDRAGRVIYIGKAGNLHSRV
                     SQYFRESGDTRPFVAILSEVLYRLETVVTATEKEALILENELIKKHRPPFNVLLKDDK
                     AYLYLRLDPGAEFPRLELSRKRRDDGALYFGTSHSAQAIRQTHLMVTRHFGLRTCADA
                     QFASRRRPCIEWQMKRCAGPCCKMVTREGYRERVDAAILFLKGRYQEVLKALTAGMKA
                     AAAAEDFEEAARLRDQVRAVQDALSRQSVVLPGTTDVDAIGIAAAGDLAVVMILRFES
                     GVLRDQAPLVLDNVVAPVAELLESVLLQFYSRTPVPAEILVPDHVGTGTGDDRPLEAL
                     ESLLAARAGRQVLIRTPKRGRSADALRMANDNAAETLRAAIEGAGSRKRALERLAATL
                     GLDRPPTRIEGFDMSQFQSAEPVGSMVVMTDGRLDKKCYRTFSVKWESSRGDTGFMTE
                     VLSRRLADVGRSGDGSDCAAVPDLIMLDGGPSQLMAARAVLDNLGLRIPLVALAKSRV
                     VAMPGSSVSHTPERLFVPADSGAPESPGLRQDERAYFSDAPDDLCDQARMLVPSQNDP
                     GLHLLMRLRDEAHRFAISFHRKRRNRRGLGSILDGINGLGKKRRTALLRHFRTVAAIR
                     QATLEDLMAVPGIPAAVADAIFDRMHQ"
     gene            7066..7905
                     /gene="larE"
                     /locus_tag="PKH54_02555"
     CDS             7066..7905
                     /gene="larE"
                     /locus_tag="PKH54_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOC98797.1"
                     /translation="MKGDQSRLPSETAARLALLREDLAARDRLAVAFSGGVDSSMLLT
                     AAASVKGLEVIAVTAVSEFLAGRELKLAGALAEELGVRHVVAEFDPLGHPEIRKNPLD
                     RCYYCKAATYRTCLDAVARHGFTTMADGTIADDAADFRPGRRAATELGVISPLADSGM
                     TKADVRVLSREIFGLKGWNRQSGACLASRFPPGTAISAGALARVEQLENALLDLGLDV
                     VRARYFGNTARIETSAEGLERLADATVRQAAVDAGIGAGFSVVAVDPEPYRPGRLNGE
                     AAL"
     gene            7902..10265
                     /locus_tag="PKH54_02560"
     CDS             7902..10265
                     /locus_tag="PKH54_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98798.1"
                     /translation="MTTAAILAQLRRTEVQWTLVPAAAAAGLLFVPGFDVLSFYFCMP
                     MALLLAMAAGSVTITAVFRGRAGGDTAAGLRRGLLHSALLGLPPLAVITAGHFINGPC
                     DYAYGLVHFMAGPFLSTIIGSGVAASCSIASRKESRASRAFIAIFILSFGPAAAILYF
                     TPAVSFFNPFLGMYPGPIYDEIIEVTPAYVTFRVWCLLAVAAFWTLARATNPGEPGMR
                     EGSRAARILVPAGLLLLCVATAYGWGNFRFLVTSGDIRRELSFVRSDKWCVLHADASY
                     AARDGLADDLLKDCGFRHRQTAEFFGVSPNPPVNVFLYRDADQKARLMGARHVEVSKP
                     WLNEIHVTGILPGEMILAHEIAHVTAGRLSPNLLAMPLRFGVVPDMALVEGLAVAAAF
                     ADDGPSPHEWALAMMRSGMTPDLGELFAPLSFITGGAAASYNTAGSFLHYVRQAHGMP
                     AIRALAQGQSFIQATGLGLDALQVRWQDFLTDQHGAEMDEVLKMRAAARFSDPGVLHR
                     KCPLDVARRLRDAAQLYERGCLEEALGKVRQAVELDPMDRSVTRLAARMEFGLARGLG
                     EHPRFIALIEDVLRPPNGAASMADRTAAADLLILRGINDIDRNRGNASLQRAFVELRD
                     LKAMLTPGGAMRGVCARLAALGASPRAASAIIEALSDPRPWAEGDAGRLYAAASESPG
                     EAVLWYLAGRAASREGLPGAAVEAFRMAIDAGFEEPLPDLADESASCASDFLAETQKL
                     LGAAAYWAGDFRTATEALSGALESARFQGDRLVILEYLERIKLEAPR"
BASE COUNT         1922 a         3360 c         3247 g         1895 t
ORIGIN      
        1 aaagactccc ggggccgatg tggcgccggt tctgatgacc gccggcagtc catgcccgac
       61 gcttcccgcc agatccctgg cgtgggttcc atccggcagt ccggccggcg cgcccccgtc
      121 gacctgaaca ccgaaccacc cggacagcct ggatgccgcg agcatgccat accatgagca
      181 gcacgaggcc cgggcaaccc tttcaagatc ctcccgggct tccaccgaac gccccatgcc
      241 gaaaagggtg acgccacgaa aatacctggc cctttcggcc agcccgaaca ttactccgtt
      301 gccacgatcc ctggtttcaa gtatcgcgtc cagatgagca agcgcctgtt cacgccttcc
      361 ggccatcatc gcggcaagtg ccttcatcca gtcggaattg cgggccagcg ccggggccat
      421 gacggatttc gacgccgctt cgaggatctc actggcccgg tcgtgaagtc cgaccctcat
      481 gaaagcgcgg gcgccgtccg aggccgctcc gggcgccaga gggcttgccg gatatccgtc
      541 cacaagcctc gcgagccact cgccggccgc ggcgtcgtca ccgccgtcca tggccgcttc
      601 cgcggcaagc ttgattatcc atgcccggac atcgggactg gacgcccgcc ccagggcgac
      661 ctgaagatcc cccgccgtgg aatcggcaat cctgtcacgg tatatcaggc acgccatcga
      721 ggccgcttcc ggcaatgccg gatcgattga cgacagtgcc ttccgattgc gcgcaatcct
      781 tcggcagagc ggtttcaaat ccctgcgcct cgaagattct atttccctga tgacctcgat
      841 tgccggcgaa aggggacttc ccagggactt cagaagggca gcagcatccc tggaatgact
      901 gccgtcacga tcgagaaacc aggcggcaag agccatggcg accgcttcac cgcgccttcc
      961 ggctgcgtcc agcgcgcggg cgagggcaag cctggccgat aggatttcat ggggcctcat
     1021 ctcgtcgtgg aaaagttcaa ggatgacctg aaccgccgct tccggccgat tctcggccat
     1081 caggcttccg gcaagcttcc gtcgggcctc gacccacatc gccgaagccg gtgaaacggc
     1141 gccccatgcg gcgcgggcac cagcgatgtc gttcttctga tccaggagca ccccgatgcg
     1201 gaaggatgcg acatcgccca gcggagaacc ggccgcggcc cgatagcggg cgattgcggc
     1261 gtcaatcctt cccgcctgct cgtaaaggcc ggccacgacc cagtcaatca cgccgccggg
     1321 aacccggcca ggctcgcact cggaaccgcc cgacctgatg gcatcaagtc ttgcaaccgc
     1381 ggcatcgatt ccgccgcgac ctttgacagc ccccgcgata agcccctgcc aggctggatc
     1441 ccttgcgatg acgatctccg actccggtga cgccgcccgc gccgaaacgc agatgaacat
     1501 tgccgcgagc agcacttcca acgtccggac cggattcatc aaacctcctc gtcaaggcag
     1561 gccgtggcag gttatcataa cagcgtttcc ggcaatcgga cggaccgttg gccgacgcgg
     1621 tcctcctgga ggtgccgaac tggacatttt cgaccccggc atgctgcagg acgcgctgac
     1681 cggcggacgc caggcggccg cgctggcggc gctctttcac gcatccctga tcgagtacgt
     1741 atttcccgtc tttccaggcg acaccgtgac catcgccgga gccgttcttg ccgtcaccag
     1801 cgggctgccg ctctggggtg tcttcgccgc cacgatggcc ggcggcctcg ccggttccgc
     1861 gctggatttc tggatcggcc gtgccttcgg tcgtggttgg aagacggcag gaccggaatt
     1921 caaaaggcga cacccctggc tcgaccgcgt cctgggccag gccgatcggc tcagcaccag
     1981 gttcgcaaga cacggaacgg tgcttctgct tgccaaccgc atgatgcccg gaatccggag
     2041 cctcctcttc gtcgctgccg gaatggccgg catgtctttc gtcaaatcga tgcttgccgg
     2101 actcgtgagc aacgccgcct ggacaggatt gctgatgctc gccgggctgg ccctcggggc
     2161 caacctcgac tcggtggtca tgctgtttcg aacttactcc gtcgccgtct ggtgcgtgat
     2221 tgcagccgca cttggccttt ggctgacggc ctggatcagg cgccggagga cggatcgacg
     2281 ggaagcgggg ccttgacgcg acgccttgtc tcgggaagcg cattgtaggc ggaaacggcc
     2341 gcggccttca tgtccggctg gcgcccgttc gccacttcgt cctgcattaa cttgtacgcg
     2401 ttcttgaaaa tgaccggcga aaccgattcg accttgtcaa cctcgccaat ctcccaggcc
     2461 ctgcgtccgt ccttcatcgc ctgcgcgatg atttcatccc tgtccgcgcg tgccggcgcc
     2521 ttcatgacca ccgtcgccag cacccggtaa gaatcgacga atggtcgcaa tatctcggaa
     2581 taccagttta tctcgtccat acgcatccgg ttgacctgga taccgtcgga caccctgaca
     2641 agcagccctt catcgacgaa aaaatcgacc gtccggcttc gtgattccag cattgaaacg
     2701 gtcttgtcgt aaacgaattc cagcctgaaa agccttgtca gatcccgcgt gcggcgatgc
     2761 agttcctgct cgtccatcct gtcggcggct gccagcgact cgaccgccgt cgccactatc
     2821 gcctgcggga ggaagaaatg gacgataccg ttcttgtagt actcaagcca cgcgcggtga
     2881 cgctcgacaa cggaaatcac ggaatcgaag ccttcgccgc tgaccttgac cgcaccctcg
     2941 cgcgcgaaca cgccaaggac ttcctcgatc aggccgtcaa ttgcggtgat ccttggcgcc
     3001 tcgagggtcg gaagtccggg cctctgcatc tcggcagtgt cacgccagtc cacataaacc
     3061 cgacgccacg cgccggtcga aaacttccca tccccgggca gcatggccgc gtccagcctg
     3121 tcggaaaaca ggccgccacg aatcgcgatc cgccggtaaa gttcgacggt ctgttccacg
     3181 agaaccgggc gcgggatgcc acgtgccctg gtcgtcatca ggacgaacgc caccaggttc
     3241 tggggagtca cgacgatgcg gctgttaata tcccacatca gcgagtatcc aaggcgcctt
     3301 acaagcatgg gatcgactga cgcgccatcc tcgagcggta tcgcgacgcc ctggccgcca
     3361 agccaggcgg tcatgctgac cggctcgccg aacttcatgt aaagctggcc gaaccttgat
     3421 cccaggttgc gggcgcgctt catgatgttc gccaggctga ccaccccgat cccggacttg
     3481 tccccgcggc gctcgcgctc gaagaacgac ccttcgacga ttcgctcgta cgtcacggaa
     3541 cagggcacca gccacacgtc cggcgccgcg ccggaagcaa cggcatccac caccatcgaa
     3601 aggatttctc cgcgcggcgg ttgcagcttg cccgacctgc tcctgcttcc ctcgatgaaa
     3661 aattccagcc agtagccttc cttgaccagc ttccttacgt actggcgaag agtcctttca
     3721 taaaccggat tggagccgat gcggtgcctg acgaagaacg cgccggtccg ccgcagcacg
     3781 ggcttgacga aattcagctt gcgctgctcg cggaccgcga catgcggcgg aatcagcccg
     3841 tgcgtataaa agacgaatga atacacgaag taatccagga tactccgtct tgacggaacc
     3901 aggacgaccg gggcattctt cccgacctcc ctgattctgg ccaggcccgc ggtatcgacc
     3961 gatatcccgg tgaacctgcg ctgaaagatg accgccagga cgaccccgaa cgcatccagc
     4021 cagccgacgc tgaaatcggc agccatgttg cgcagccatg cccccgcctt cttcatgctg
     4081 gcggccggtc cgagtccgac ctgcgcgccg gccgtggtga cggcatcctt gaacggcctt
     4141 gtccgcatga tctcatcgac cattacgccg cggcctttca gaaccggtcc ccggatggcg
     4201 cgggactcgc gcagcagcgt ctcggcaatc gcgaacttca acctgacggc aacggcatcg
     4261 ggatcgcgat cgggcgactc ctggacgaat gaccgcagat cgatcggact tccaacctga
     4321 gcatgggcgc ttcgaaaatt ccagatgaac gaaagcgcct tgcgcagggc cccgggcgcc
     4381 tggggatcgc cgaagacgaa gtcgagcacg ctcctgcggt aggactccgg tttctgttcc
     4441 cagaccagca gaagcgggat gatcatcagg ggacgctcga cctcggactg tatctcgacc
     4501 agatccttaa ggtagctgat ccggtattcg gcgccccact ggctgagcgc cttcggccgc
     4561 ttcagaaaga tcaggcacgg gcggccggcg ccaagcccgg cgcgcatcat ctcgctgtcc
     4621 ggcagggggg gaaagccccg gccggtcaac ctcatccaga ccctgcgaag atactgggac
     4681 atcggcctga atgcggacat cgagatttcg ttggcgaaaa agacaagcgg aagcccgaaa
     4741 cgggcaaagg cataattgaa atacaggtag tcgagacggc tgtgggcatc catcacgaac
     4801 accggcgtgc ccgcgctgca tgcaatccta atcgcatcga tattcgattc gtcaaacctg
     4861 accgcaccga agaaagtgcg gctgagccaa cgcatgaatc ggccctggcg cctgaccatg
     4921 gaagaatccg actcggtgtc gccgtcaagc acagggggct gctgacggcc ggaaagaacc
     4981 ggcgtcgtct tgtcatcgga cttcatcgcg atacctgaag aaccggccac ggatcataca
     5041 ccaagttatc gacgcggcgg ttccgattcg ccaaactcct gtatcattga agaccgttgg
     5101 ttccgggggc gtcatgggct ggagtcccga aaaggcagcg gccttcacgg cggatcttcc
     5161 gcacaggcct ggcgtctata tcatgcgcga ccgggccggg cgggtgatct acatcggcaa
     5221 ggcgggaaac cttcattccc gggtcagcca gtacttcagg gaatccggtg acaccaggcc
     5281 gttcgtcgcg atcctttcag aagtcctgta caggctggaa acggttgtca cggccaccga
     5341 aaaggaagcc ctgatccttg aaaacgaact gatcaagaag catcggccgc ctttcaacgt
     5401 tctccttaag gatgacaagg cgtatctgta cctgaggctg gatcccgggg ccgaatttcc
     5461 cagacttgaa ctcagcagga aaaggcgcga cgacggggcc ctctacttcg gcacgtcgca
     5521 ctcggcccag gcaatacgcc agacacacct gatggttacc aggcacttcg gcctgcgaac
     5581 ctgcgccgac gcgcagttcg caagccgcag gcgcccatgc atcgaatggc agatgaaacg
     5641 gtgcgccggg ccctgctgca agatggtgac ccgggaggga taccgggaaa gggtcgatgc
     5701 ggcaatcctc ttcctcaaag gcaggtacca ggaagtgctg aaggcactga cggcggggat
     5761 gaaagcggcg gcggcggccg aagacttcga ggaggccgcc cgcctgcgcg accaggttcg
     5821 ggcggttcag gacgcccttt cccggcagtc ggtcgtcctg cccggcacga cggacgtgga
     5881 cgcgattgga atcgcggcag ccggggatct ggccgtggtc atgatcctga ggttcgaatc
     5941 gggcgtactg cgcgaccagg cgccactggt gctcgacaac gtggtggcgc ccgtcgcgga
     6001 actgcttgaa agcgtgcttc tgcagttcta ttcgcgcacc ccggtgccgg ccgaaatcct
     6061 ggttccggat cacgtcggga cgggaaccgg tgacgatcgc ccgcttgaag cgctggaaag
     6121 ccttctggcg gccagggccg gcaggcaggt tctcatcagg acgccgaagc gcggccggtc
     6181 ggcggacgct ctgcggatgg cgaacgacaa cgcggcagaa acccttcggg cggccatcga
     6241 gggcgcggga tcccggaaaa gggcgcttga acgccttgcg gccacgctcg gcctggatcg
     6301 gccgccgacc aggatcgaag gcttcgacat gagccagttc cagtccgcgg agccggtcgg
     6361 atcgatggtc gtgatgaccg acggacggct cgacaagaag tgctatcgga cgttttccgt
     6421 gaaatgggaa tcaagccggg gcgacacagg gttcatgacg gaagtgcttt cgcggcggct
     6481 ggcggatgtc ggccggagcg gcgatggatc ggactgcgcg gccgtgccag acctgatcat
     6541 gcttgacggg ggaccttccc agctcatggc agccagggcg gttcttgaca acctggggct
     6601 tcgaattccc ctggtggcgc tggccaagtc cagggttgtc gcgatgccgg gctcgtccgt
     6661 ttcccatacc cccgaaagac tgttcgtgcc tgcggacagc ggggcgccgg aatcccccgg
     6721 gctgcgccag gatgaaaggg cgtatttttc ggacgcgccg gacgacctgt gcgaccaggc
     6781 caggatgctt gttccaagcc agaacgatcc cgggcttcat cttctgatga ggcttcgtga
     6841 cgaggcccac cgattcgcga tttcgtttca caggaagcgg cgtaaccgga ggggcctggg
     6901 ttcgatcctg gacgggatca acggtctggg aaagaagcgg cgcacggcgt tgctgcgcca
     6961 tttcaggacg gtggcggcaa tccgccaggc aacactggaa gacctgatgg cggtacccgg
     7021 cattcccgcg gccgtggctg atgccatttt cgacaggatg caccaatgaa aggcgatcaa
     7081 tcccgcctgc catcggaaac ggcggcaagg ctggcactcc tgagagaaga cctggcggcg
     7141 cgggacaggc ttgccgtcgc gttttccggc ggcgtcgatt catcgatgct gcttacggcc
     7201 gcggcttccg tgaaggggct ggaagtcatc gcggtcacgg ccgtgtccga gttccttgcc
     7261 gggcgtgaac ttaagctggc gggggccctt gcggaagaac tgggggtcag gcacgtggtc
     7321 gcggaatttg atccgctcgg gcatcccgaa atccggaaaa atccgctgga tcgttgctat
     7381 tactgcaagg ctgcgactta caggacctgc cttgacgcgg tcgcccgcca tggttttacg
     7441 acaatggccg atggaacgat tgcggacgat gccgcggact tccgccccgg ccgcagggcg
     7501 gcgacggaac tcggggtgat aagcccgctg gccgattccg ggatgacgaa ggccgatgtc
     7561 cgtgtgcttt cacgggaaat cttcggcctg aaaggctgga acaggcaatc cggtgcctgc
     7621 ctcgccagcc gcttcccgcc cggaaccgcg atttcggccg gggcgctggc cagggttgaa
     7681 cagttggaaa atgcccttct ggatctcggg ctggacgtgg tgcgggccag gtatttcggg
     7741 aacacggcaa ggatcgagac ctctgccgaa ggcctggaac ggcttgctga cgcgaccgtc
     7801 aggcaggctg ccgtggacgc cgggatcggt gccggctttt cggtcgtcgc ggtggatccg
     7861 gagccctacc ggcctggacg cctgaacggg gaggccgcac tatgacgacg gccgcgatac
     7921 tggcgcaact tcgcagaacc gaggtgcaat ggaccctggt tcccgcggcg gccgcggccg
     7981 gattgctttt cgtccccggg tttgatgtcc tgtcgtttta tttctgcatg ccgatggccc
     8041 tgctgctggc gatggccgcc ggttcggtta cgattaccgc agtcttccgt ggcagggctg
     8101 gcggggatac cgccgccggg ctgcgacgtg gattgctcca ttcggcgctg ctgggcctgc
     8161 cgccgcttgc cgtaatcacc gccggtcact tcatcaacgg gccgtgcgat tacgcttacg
     8221 gacttgtgca tttcatggcc gggccgttcc tttcaaccat catcggctcc ggcgtcgcgg
     8281 cgtcctgctc gatcgcttcg cgaaaggaat caagggcatc gcgcgcattc atcgcgatct
     8341 tcatcctgtc tttcggcccg gctgccgcaa tcctctattt cacgccggca gtcagcttct
     8401 tcaacccgtt cctggggatg tatccgggac cgatttacga tgaaatcatc gaagtcaccc
     8461 ccgcctatgt gacgttccgc gtctggtgcc tgctcgcggt ggctgccttc tggaccctgg
     8521 caagggccac gaacccggga gaaccgggga tgcgggaagg ttcgcgggca gcgaggatac
     8581 ttgtccccgc cggtttgctg ctcctgtgcg tggcaactgc atacgggtgg ggaaatttca
     8641 ggtttctcgt caccagtggc gatatccgtc gggagttatc cttcgtcaga tccgacaaat
     8701 ggtgcgtcct tcatgccgac gcgtcgtacg cagccaggga cggacttgcg gacgatcttc
     8761 tgaaggactg cggcttcagg cacaggcaga ccgccgaatt cttcggcgtg agtccgaatc
     8821 cgccggttaa cgtcttcctg tatcgcgatg ccgaccagaa ggccaggctg atgggtgcca
     8881 ggcacgtgga agtcagcaag ccctggctca acgaaatcca tgtaaccgga atactgcccg
     8941 gagaaatgat cctggcccat gaaatcgcgc atgttacggc cggacgcctt tcaccgaacc
     9001 tgctggccat gccgcttcgt ttcggggtgg ttccggacat ggccctggtc gagggtcttg
     9061 cggtggccgc cgccttcgcg gacgacggcc cttcaccgca cgaatgggcc ctcgccatga
     9121 tgcgatcggg catgacgcct gacctggggg agcttttcgc gccgctttcc ttcattaccg
     9181 gcggtgcggc ggcctcgtac aacaccgccg gttccttcct tcattacgtc cgtcaggcac
     9241 acggcatgcc ggcaatccga gcgctggcgc agggccaatc cttcatccag gccactggcc
     9301 tggggcttga cgccctgcag gttcgctggc aggacttcct gaccgaccaa cacggcgccg
     9361 aaatggacga agtcctgaag atgcgcgccg ccgctcgttt ctccgacccc ggcgtccttc
     9421 acaggaagtg tccgctggac gtggcccgaa ggctgcgcga cgccgcgcaa ctctacgaac
     9481 gcggctgcct ggaggaggcc ctgggcaagg tacgccaggc cgtcgaactc gacccgatgg
     9541 atcgttctgt gacaaggctg gccgccagga tggaattcgg actggcgcgt ggccttgggg
     9601 aacatccccg attcattgcg ctgatcgagg acgtcctccg accgcccaac ggcgccgcat
     9661 ccatggctga tcgaaccgcc gcggccgacc tgctgattct gcggggcatc aacgacattg
     9721 accgtaaccg cggcaacgca agtcttcaaa gggctttcgt ggaactgcgc gatcttaagg
     9781 cgatgctgac ccccggcggc gccatgcggg gtgtctgcgc gcggctggcc gcccttggtg
     9841 ccagtccccg ggcagcctcg gcaataatcg aggcgctttc ggatccgcgt ccgtgggcgg
     9901 aaggtgacgc gggccgcctt tacgcggccg cttcggaaag ccccggggag gccgttctat
     9961 ggtacctggc cggaagggcc gcgtcccggg aaggactgcc gggagccgcg gtcgaggcat
    10021 tcaggatggc cattgacgcg ggttttgagg aacctcttcc cgatttagcg gatgagagcg
    10081 cttcctgcgc gtcggacttc ctggcagaaa cgcagaaact gcttggcgcg gccgcgtact
    10141 gggccgggga tttcaggaca gcgaccgaag ccttgtctgg cgcgcttgaa tcggcacgct
    10201 tccagggcga tcgactggtg attctggaat accttgaaag aattaagctt gaagccccgc
    10261 gatagcctgt caggccgacg tgccctgacc cccggaatcc ggccggatgc gccttgaaaa
    10321 tgtgattacc ccgtgcgcga ccatgccggc ctgggccagc aggtacatcg cgatcgatac
    10381 ccagatcagg tgcatgtcgg tcctgaaccg gagggcgccc tcta
//
LOCUS       DAOPPA010000049        10414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7290_length_10414_cov_4.338353, whole genome shotgun sequence.
ACCESSION   DAOPPA010000049 DAOPPA010000000
VERSION     DAOPPA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7290_length_10414_cov_4.338353"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2533)
                     /locus_tag="PKH54_02565"
     CDS             complement(<1..2533)
                     /locus_tag="PKH54_02565"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOC98799.1"
                     /translation="MPDSVPDVVPVPVIPGPLPVEPGVADAHLQDLGSVEPDSVEPEP
                     VEPDFAEHAFSGDGSPVDVSEGESVCDFPDEGVEPVVCQGAGVDGEGLVEEGIEKMSS
                     KGGLDSSMGKVMPYGRYFTREGQDIFSTVTWEKRDAKIVAHDGAVVFCQTGMEFPDFY
                     SLTASNVVASKYFRGTEGGPDRESSLKAMITRVVETIAGWALQGGYFADQDAVDVFAD
                     ELAWLILHQYGSFNSPVWFNVGVEDHPQCSACFINSVEDTMESILGLAVTEGLLFKFG
                     SGTGTNFSTIRSSRERLKGGGIPSGPVSFMRGYDAFAGVIKSGGKTRRAAKMVVLNAD
                     HPDIVDFIECKVREEDKALALIREGYDGSFGGEAYDSIFFQNANNSVRVTDEFMNAVV
                     SNGSWQTKAITTGQVVETMKAGDLMDRMAKAAHRCGDPGIQFDTIVNDWHTCSVSGRI
                     NASNPCSEFMFLDNSACNLASLNLMRFVDDDGEFNTAAFRHAVRTFIIAQEVIVSNAK
                     YPTEAIGRNSESYRPLGLGYANLGALLMNRGLAYDSDAGRAYAAAVTALMTGEAYVQS
                     AILAGRVGPFAEYTRNSSSMLRVIRKHRAALETVSDRLVPDVLMEAARESWDNAVALG
                     GEHGFRNAQVTLLAPTGTIGFMMDCDTTGIEPEMAIVKYKNLVGGGRMKIVNRTVPSA
                     LRKLGYTDSQIKAISDYVDRNDTIEGAPGLKDEHLSVFDCAFRATNGVRSIAVDGHIH
                     MMGAVQPFLSGAISKTVNMPADATVEDVRKAYIDAWKYGLKAVAIYRDGSKGTQPVET
                     SARKEPEKPAAPAAAVPVRSKLPDERQSLTHKFTISGLDGYITVGM"
     gene            2970..4412
                     /locus_tag="PKH54_02570"
     CDS             2970..4412
                     /locus_tag="PKH54_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC98800.1"
                     /translation="MYASEDKVFDLEEAIEDGEQRTLLMDDDAVSEITSNSIMAYLSR
                     IGDTALLSREQEQSLARTIEDGEKESFLWMVRIPFCLNHLTGFPHRLTSGEVSLLDVS
                     LLDESSHEDWTPSLSKELQDFADRIDGISGSVRGGEVHDEYVLSLNLYNAYHEFKFGA
                     GIVRLVLRSLQDQLRRLSDDARGINTMDEADASAFGALDLNAGVISGAASAKAAGMVL
                     GVRGDALREAVTGLTRAQARAEKARSWMIRANLRLVVSIAKKYVNRGIPLLDLIQEGN
                     IGLMKAVSRFDWRLGHKFSTYATWWIRQSISRTLAEHGKTIRVPIHLVECVNKVRRAR
                     ARLRVQNGAEPTMEEIAAETGYTAEQVFRAEKTILSAISLETPIGDDDNKLMDVVEDT
                     NSRQPFEETCDENLSACVRRLLAGLSPKEEAVVRLRFGIGCKREHTLEEVGEAVGLTR
                     ERVRQIEVKALEKLRAPAAGRDMGSFLVDA"
     gene            4671..6224
                     /gene="lysS"
                     /locus_tag="PKH54_02575"
     CDS             4671..6224
                     /gene="lysS"
                     /locus_tag="PKH54_02575"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOC98801.1"
                     /translation="MIETSENTLIDQRLEKLQKLREVDGAAYPNDFRVTESIATVRAR
                     GIGPDGEPLSNEALEAAAIQVSVAGRAMAVNSFGKAAFIRVRDRSDTMQVYVKKDEVD
                     ETSFTRFKLLDIGDIVGVEGELFVTRTGELSVKAKSFRILSKSQRPLPEKFHGLQDAE
                     MRQRMRYVDMIVNDDSREVFRTRTRIIKYIRDYLDQRDFIEVETPMMHPILGGANARP
                     FVTHHNTLDVDLYLRIAPELYLKRLVVGGFDRVYEMNRNFRNEGFSNRHNPEFTMIEL
                     YQAYATYLDLMDMTEDLFQSMARDILGKTVVQFRGHDIDLGKPFRRLPVCEGLREYAD
                     APEQAFHDGRAAYEWGLKHRIDRKAADIVMKKYQDSRDPNAQRNCALEICMMVFEETV
                     EQHLIQPTFVTDFPAAISPLSRRKDSDPTLVDRFELYVATNEFGNAFSELNDPQDQEE
                     RFRAQMVAKAAGDQETMEYDEDYIRALQYALPPTAGEGIGIDRLAMLMTGSDSIRDVI
                     LFPMLRPEK"
     gene            6288..7964
                     /locus_tag="PKH54_02580"
     CDS             6288..7964
                     /locus_tag="PKH54_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOC98802.1"
                     /translation="MLEGLLERAGAAIGYMLPAVQLTGVILASLIMTAVLVGMIVRAL
                     KRFSERGGAGSWKLLLALRFLLGFREEKRSWFRRLVPVATQNFIAMTGTAIGVWALIV
                     VLSVMSGFELDLKDKIIRNNPHITVERCAASADDFDGGQTCQPMADRIGRIPDVVMAE
                     SFVQGDALLASSSNMGPGTTIRGITPGGALEARWLRNSVSRGALEGLVRPETTISDRD
                     LGFVFPDPATRQAAEPGEAAVQPPARDGIAPSQPSEGRKAEDAATVGVETAELNVPPI
                     AAPDEIDVPPIAEPDDGVRVRDGIILGSELALGLGVTEGDEVTVVIPDGDVGPLGLQP
                     STSVFRVVGTIKSGLYEFDLKTAFVLADSGRRLFSGASREQMAIMVSDIQKLEGIASE
                     VQAVADGVPGSRVRTVTQTNRSLFSALMVERIAMFLVLGLVILVAAFNVFGSLLLVTM
                     EKTRDIAVLRSMGVTASGIRSVYFTIGWVIGLTGTVSGLALGLLTCLYIKTAGIALPQ
                     EYYIERLPVSVSALDVALVALAALGAALIATIYPTSAVASMSPADGLRND"
     gene            8027..8680
                     /locus_tag="PKH54_02585"
     CDS             8027..8680
                     /locus_tag="PKH54_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC98803.1"
                     /translation="MFSHGGREINVLRGLDLVMEPREMTAILGKSGVGKSTLLHILGT
                     IDKPDGGTIRFDGIDVFKLDEPGLAAFRNKRIGFIFQFHHLLPEFSALENVMIPAIIG
                     GLSRREADERATDLLTTVGLEHRMTHRPSELSGGEQQRVALARALVMRPMLIIADEPT
                     GNLDDATSEEVHNLFLEVNERFGVAFLVATHNMKLAGRMHRILKLEDGKVHPVGGDA"
     gene            8677..>10414
                     /gene="bamA"
                     /locus_tag="PKH54_02590"
     CDS             8677..>10414
                     /gene="bamA"
                     /locus_tag="PKH54_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOC98804.1"
                     /translation="MKRTLTVAALLLFVAVACPAAAEPLQARAPGAAEIDPIEDAEPE
                     EPEEEAPAQAAAASATPDAKIIREVRFTGLLRTDRDTVLRRVKSGVLSELDPARVSRD
                     IKALYKTGAFETIQARAIPEEDGVILVFEVKEYPAVAVVRTEGNKKIDNDKLKEKIVV
                     RESTILDMRKVERSVEAIKAHYVEEGYYLAEVSYRLDPKPDNVVDVVFVIREHSKVKV
                     RSIEFLGNHNIPDQDLKKFMQTQEGDLLSIVSSKGLFAKENLEMDVRRLEFFYNTKGY
                     AEVKIEEPVVMLSRDRKHIAIAITIHEGPQYKVGKVDIGGDIIFPKEDLMKKLALKEG
                     DIFNAENVQKDAMGLLTKYKDEGYAFCNVGNTTNMDQKNLILDFTYIIQKGVKARITR
                     IDITGNEGTRDWTIRRELRVYEGDMFNETQMKRSEARIRRLGFFEEVEIVPRQGDGPD
                     QVILTVRVKERQTGAFSIGAGFSSVENFLFQAQISKQNFLGRGQSLRLEASLSSIRKY
                     FVFSFEDPNFFDSEWTFGFSAYNRDYINYTYDENSTGMSLTIGRRFADYFSVAASYNL
                     EMVDVKAGGQEDF"
BASE COUNT         2225 a         3099 c         3075 g         2015 t
ORIGIN      
        1 gcattccgac cgtgatatat ccgtccagcc cgctgattgt gaacttgtgc gtaagcgact
       61 gacgttcgtc gggcagcttc gacctgaccg gaaccgcggc ggccggggcg gcaggctttt
      121 ccggttcctt cctggccgac gtctcgaccg gctgggttcc cttcgaaccg tccctgtata
      181 ttgccaccgc cttaaggccg tacttccacg catcgatata cgccttcctg acgtcctcga
      241 cagtggcgtc ggccggcatg ttgacggtct tcgaaatcgc gcccgaaagg aacggctgaa
      301 ccgcgcccat catgtgaatg tgcccgtcaa cggcgattga acgcacgccg ttggtggccc
      361 tgaacgcgca atcaaagacg gaaagatgtt catccttcag gccgggagcc ccttcaatcg
      421 tgtcgttcct gtcaacgtag tcggaaatcg ccttgatctg ggaatcggtg taacccagtt
      481 tcctcaaggc ggacggaacg gttcggttga cgatcttcat gcggccgccg ccgacaaggt
      541 tcttgtactt gacgatggcc atctcgggct cgatgccggt agtatcgcag tccatcatga
      601 acccgatcgt gccggtcggc gcaagaagcg tcacctgggc attcctgaac ccatgttcac
      661 cccccagggc aaccgcgttg tcccaacttt ctcgggctgc ttccatcagg acgtcgggaa
      721 caagcctgtc gctcacggtc tcgagcgcgg cgcgatgctt gcggatgacc cgcagcatcg
      781 aggatgaatt ccgcgtgtat tccgcgaacg ggccgacgcg gccggccagg atggcgctct
      841 ggacataagc ctcgccggtc atcaacgcgg tcaccgccgc cgcgtaggca cggccggcat
      901 cactgtcata cgccagcccc cggttcatca gcaacgcgcc gagattggcg tatcccagac
      961 caagcggccg ataactttcg ctgttgcgtc cgatggcctc ggtcggatac ttcgcgttgg
     1021 acacgatgac ttcctgggcg atgatgaaag tcctgaccgc gtgacggaaa gccgccgtgt
     1081 tgaattcccc gtcgtcgtca acgaatcgca tcaggttcag cgaggccagg ttgcacgcgg
     1141 aattgtccag gaacatgaac tcggaacaag ggttggaagc gttgatcctg cccgacacgg
     1201 aacaggtgtg ccagtcgttt acgatcgtgt cgaactggat tcccgggtcg ccgcagcgat
     1261 gggccgcctt cgccatcctg tccatcaggt cgccggcctt catcgtctcg accacctggc
     1321 cggtcgtgat tgcctttgtc tgccaggaac cgtttgaaac gacggcgttc atgaattcgt
     1381 cggtcacgcg aaccgaattg ttggcattct ggaagaagat agagtcgtac gcctcaccgc
     1441 cgaacgaacc gtcataacct tcgcgaatca gggcaagtgc cttgtcttcc tcgcgaacct
     1501 tgcattcgat gaaatcgacg atatcgggat gatccgcgtt cagcacgacc atcttggcgg
     1561 cccgcctggt cttgccgccg ctcttgatga cgccggcgaa cgcatcgtat ccccgcatga
     1621 acgaaaccgg gccggacgga atgccgccac ccttcagtcg ttcccgggaa gaacggattg
     1681 tcgaaaaatt ggtgccggtt cccgatccga acttgaaaag caggccctcg gtcaccgcca
     1741 gacccaggat gctttccatg gtgtcttcaa ccgaattgat gaaacaggcc gaacactgcg
     1801 gatgatcctc gacgccgaca ttgaaccaga ctggactgtt gaacgaaccg tactggtgca
     1861 gaatcagcca tgcaagctcg tccgcgaaga catcgacggc gtcctgatcc gcgaaatatc
     1921 cgccctgaag cgcccagccg gcgatcgttt ccaccacgcg ggtgatcatt gccttaagac
     1981 tggattcgcg atccggaccg ccctcggttc cacggaaata ctttgaagcg accacgtttg
     2041 aggcggtaag ggaataaaaa tccgggaact ccatcccggt ctggcagaag acaacggcac
     2101 catcgtgcgc gacgatcttc gcgtcgcgct tttcccacgt caccgtcgaa aaaatgtcct
     2161 ggccttccct ggtgaaatac ctgccgtatg gcatgacctt gcccatggaa gaatccaacc
     2221 cgcctttgct gctcatcttt tcaatccctt cttcaaccag cccttcacca tcgacacccg
     2281 caccctggca cacgacgggc tcgacacctt catcgggaaa atcgcagact gactcaccct
     2341 ctgaaacatc aaccggggag ccgtcgcctg aaaacgcatg ctccgcgaaa tccggctcca
     2401 ccggttccgg ctccacagaa tccggctcca ccgatccgag atcctgaaga tgcgcatccg
     2461 caacgccggg ctcgacgggc agcggcccgg gaataaccgg caccggcacc acatcgggga
     2521 cagaatcagg cacggattcc ccgcccccgg ctccaaacaa gtcatcttcg ttccagtttc
     2581 cagccatacc gatccgccag attggtccag acaaacgtca ccgcgacgaa tggcgatcgt
     2641 aaccccgcct tgtgggacgt caagaaaaaa ccactatatg ttggggttat tcgacaggta
     2701 tcccccaaat gattgtgttt cgaggagtta caccgcactt tcaacagtcg cgatttcagc
     2761 ccttccgaga accgcgacat cggacgaatc atcgcgacat ttttgtcacg atccggtgcg
     2821 cttcggcggg ccctgccgaa gcccctcgcc tgaaacactt tcgcgtttgg ttcgaaatac
     2881 cttttatttc cttaacttca gaattcgtcc cgaaggtggc ccgcaatatg caatacaaac
     2941 cggcgtggac ttccccgtgg aggtgttcca tgtacgccag cgaagacaag gtttttgacc
     3001 tggaagaggc catcgaggat ggcgaacagc gtacccttct gatggacgac gacgcggttt
     3061 ccgaaatcac gtcgaattcg atcatggcct acctcagccg cattggcgac acggctctgc
     3121 tctcccgcga gcaggagcag tcgctggcaa gaacgatcga ggacggcgag aaggaaagct
     3181 tcctgtggat ggtgcggatt cccttctgcc tgaaccacct taccgggttc ccgcacaggc
     3241 tgacgtccgg cgaagtaagc cttctggacg tttcgttgct tgacgaaagc agccacgagg
     3301 actggactcc gtcgctttca aaggaactcc aggatttcgc tgacagaatt gacgggattt
     3361 ccggttcggt gcgcggtggc gaggttcatg acgaatatgt tctttccctg aacctttaca
     3421 acgcgtatca cgaattcaag ttcggcgccg gaatcgtccg gcttgtgctc cgcagccttc
     3481 aggatcagct tcgccggctc tcagacgacg cgcggggcat caacacgatg gacgaggccg
     3541 acgccagcgc cttcggggca ctcgacctga atgcgggcgt gatctcgggc gctgcctcgg
     3601 ccaaggccgc cggaatggta ctcggcgtgc gcggcgacgc actgcgggag gcggtgaccg
     3661 gccttacaag ggcccaggcc cgcgccgaga aggcacgttc atggatgatc cgcgccaacc
     3721 tgcgcctggt cgtgtcgatt gccaagaagt acgtcaaccg cgggatcccc ctgctggatc
     3781 tgattcagga aggcaacatc ggcctgatga aggcggtcag ccgtttcgac tggcgccttg
     3841 gccacaaatt ttccacgtac gcgacgtggt ggatccggca gtcgatatcc cgaacgctgg
     3901 ccgaacacgg caagacgatc agggttccga tacacctcgt cgaatgcgtc aacaaggttc
     3961 gacgcgcaag ggccaggctg cgggtacaga acggggccga gccgacgatg gaagaaatcg
     4021 ccgccgagac cggatacacg gctgaacagg tattcagggc cgaaaagacg atcctgtcgg
     4081 caatttccct ggaaacaccg atcggcgacg acgacaacaa gctgatggac gttgtcgagg
     4141 acacaaattc acgccagccc ttcgaggaga cgtgcgacga aaacctcagc gcatgcgtca
     4201 gaaggctcct tgccggcctg tccccgaagg aagaggcggt cgtccgcctt cgcttcggca
     4261 tcggttgcaa gcgcgaacac accctggagg aagtcggcga ggccgtcgga ctcacccggg
     4321 aacgagtgcg ccagatcgag gtcaaggcgc ttgagaagct tcgtgccccg gccgcgggac
     4381 gtgacatggg cagtttcctg gtggatgcct gattccacgg tttacggact gcctgcccgg
     4441 gtttgaatat cttattgccc cttcagccgg tgcccggctc aaaaacaaat tgaacgggca
     4501 ccggttcatt tgaaaccccg gttctgcgaa ttcaagggaa tggagcgaac caggcaacgc
     4561 cttacgcacg cggacgcgtg tccggacgct cgcggcgcac acgcaccggg attgacattt
     4621 ataccttcta ccggtagggt acggcgcgct ttcccgcggg agaattcaaa gtgattgaaa
     4681 ccagtgaaaa tacattgatt gatcagaggc tcgaaaagct tcagaaactg cgcgaagtcg
     4741 atggtgcggc gtatcccaac gattttcgcg ttacggaatc cattgcgacc gtacgcgccc
     4801 gtggcatcgg ccccgacggc gaaccgcttt cgaacgaagc acttgaggcc gcggcaatcc
     4861 aggtcagcgt ggccggccgc gccatggcgg tcaactcttt cggcaaggcg gccttcatcc
     4921 gggtcaggga tcgctcggac acgatgcagg tttacgtcaa gaaggacgaa gttgacgaga
     4981 cgtcgttcac aaggttcaag ttgctcgata tcggcgatat cgtcggcgtc gagggcgagc
     5041 ttttcgttac aagaaccggt gaactgagcg tcaaggcgaa gtctttcagg attctttcca
     5101 agtcccagag acctctgccc gagaagttcc acggcctgca ggacgccgag atgcgtcagc
     5161 ggatgagata cgtcgatatg atcgtcaacg acgactcccg cgaggttttc cggaccagaa
     5221 ccaggataat caagtacatc cgcgactatc tggaccagag ggacttcatc gaggtcgaaa
     5281 ccccgatgat gcatccgatc ctgggcgggg caaacgccag gccgttcgtc acacaccaca
     5341 acacgctgga tgtcgatctt tacctgcgaa tcgcacccga gctttatctg aagcgtcttg
     5401 tcgtcggtgg tttcgaccgc gtctatgaaa tgaaccgcaa cttccggaac gagggatttt
     5461 ccaaccgtca caatccggaa ttcaccatga tcgaactgta tcaggcttat gcgacgtatc
     5521 ttgacctgat ggacatgacc gaggatctct tccagtccat ggccagggac atcctgggaa
     5581 agaccgtcgt gcagttccgc ggccacgata tcgacctggg aaagccgttc cgccggcttc
     5641 ccgtgtgcga gggtctgcgt gaatatgccg acgctccgga acaggcgttc catgacggac
     5701 gggcggccta tgaatggggc ctgaaacacc gaatcgaccg caaggccgcc gacatcgtga
     5761 tgaagaagta ccaggattcc cgggatccaa acgcccagcg caactgcgcc ctggaaatct
     5821 gcatgatggt gttcgaggag accgtggagc agcacctgat ccagccgacg ttcgtgaccg
     5881 acttcccggc ggccatttcc ccgctttcgc gccgcaagga ttccgatccg acactggtcg
     5941 atcggttcga actgtatgtc gcgacgaacg aattcgggaa tgcgttctcg gaactgaacg
     6001 atccgcagga tcaggaagaa cgcttccggg cgcagatggt cgcaaaggcg gccggcgacc
     6061 aggaaacgat ggaatacgac gaggactaca tccgcgcact tcagtacgcg cttcccccga
     6121 ccgcgggcga aggcatcggc attgacaggc ttgcgatgct gatgacaggt tcggacagca
     6181 tcagggacgt aatactgttc ccgatgctcc gcccggaaaa atgaccttgt cgttgacccc
     6241 gtccctttga cgggatggaa gcggccggaa acggcctccg gggcaaaatg ctggaaggac
     6301 tgctggaaag ggccggggcg gcgattggat acatgctgcc cgcggtgcaa cttaccggcg
     6361 ttatccttgc ttccctgatc atgaccgccg tgcttgtcgg catgattgtc cgggcactga
     6421 agcgcttttc cgaaaggggc ggcgccggct cctggaagct gctgctggca ctgcgcttcc
     6481 tgcttggctt ccgcgaggaa aagcgatcct ggttccgccg cctggtgccg gtcgccacgc
     6541 agaacttcat cgcgatgacc ggaaccgcaa tcggcgtatg ggcgcttatc gttgttcttt
     6601 ccgtgatgag cgggttcgag cttgacctca aggacaagat catccgcaac aatccacaca
     6661 taaccgtcga gcggtgcgcg gcttcggccg acgacttcga cggaggacag acctgccagc
     6721 cgatggccga ccggatcggg cgcattcccg acgtggtgat ggccgaatct ttcgttcagg
     6781 gcgatgcgct gctggcgtcg agctcaaaca tgggcccggg aacaaccatc cgtggaatca
     6841 ccccgggcgg tgcgcttgag gcccgctggc ttcggaattc cgtttcaagg ggggcgcttg
     6901 agggacttgt aagaccggaa acaaccattt ccgacaggga ccttggcttc gtgttcccgg
     6961 atccggccac gcgccaggcg gcggaaccgg gtgaagcggc ggttcagccg ccggccaggg
     7021 acgggattgc tccatcgcaa ccgtctgaag gcaggaaggc cgaagatgcc gccaccgttg
     7081 gcgttgagac cgccgaactc aacgttcccc caattgcggc gcccgacgag atcgacgttc
     7141 cgccgattgc ggaaccggac gacggggtca gggtaaggga cggcatcatt cttggttccg
     7201 agcttgcgct gggacttggc gtcactgaag gagacgaagt cacggtggtg atcccggacg
     7261 gggatgtcgg tccccttgga ctccagccat cgacttcggt cttcagggtc gtgggaacca
     7321 tcaagagcgg cctttacgag ttcgacctga agacggcctt cgtgctcgcg gacagcggcc
     7381 gccggctttt ttccggagca tcccgcgaac agatggcaat catggtgtcc gacatccaga
     7441 aactggaggg aatcgcctcc gaagttcagg cggtcgccga cggcgtccca ggctcccggg
     7501 tcaggacggt cacgcagacc aacagatccc tgttttccgc cctgatggtc gagagaatag
     7561 cgatgttcct ggtgcttgga ctggtgatac tggttgcggc attcaatgtc ttcgggtcgc
     7621 tgctgctggt gacgatggaa aaaacgcggg acatcgccgt gctgcggagc atgggcgtga
     7681 ccgcatccgg aatcaggtcg gtctatttca ccatcggttg ggtgataggc cttaccggca
     7741 ccgtttccgg cctggcgctc gggctgctga cctgcctgta catcaagacg gccggcatcg
     7801 ccctgcccca ggaatactac atcgaacggc tgcccgtcag cgtcagcgcg cttgacgtgg
     7861 ctctcgtcgc cctggccgct ctcggggctg ccctgatcgc gacgatttac ccgacttcgg
     7921 cggttgcatc gatgagcccg gccgacggtc ttcgaaacga ctaggtggcg acttggaaaa
     7981 cgaaaacaga caggctccgc ttcttgaggc atccggtctt acaaggatgt tttcccacgg
     8041 cggccgtgaa atcaacgtcc tgcgcggcct ggatctcgtt atggaaccgc gtgaaatgac
     8101 ggcgatactc gggaagtccg gtgtcgggaa gagcacgctg ctgcatatcc tggggacaat
     8161 cgacaagccg gacggcggta ccatacgatt cgacggcatc gatgttttca agctggacga
     8221 accggggctt gcggcattcc gcaacaagcg catcggattc atttttcagt tccaccatct
     8281 tcttcctgaa ttctcggcgt tggaaaacgt gatgattccg gcgataatcg gcggactgtc
     8341 gcgcagggaa gccgacgaac gcgcgaccga cctgctgacg acagtcggtc ttgaacaccg
     8401 catgacgcac cgcccttcgg aactgtcagg cggcgaacag cagcgcgtgg cgcttgcccg
     8461 ggcccttgta atgcgcccga tgctcatcat cgccgatgag ccgacaggaa atctggatga
     8521 cgccaccagc gaggaggttc acaacctttt cctggaagtg aacgaacggt tcggggtcgc
     8581 cttccttgtg gcgacccaca acatgaagct ggctggaagg atgcacagga ttttgaagct
     8641 ggaagacgga aaagttcatc ccgtgggggg cgacgcttga aacgaaccct tacggttgct
     8701 gccctgctgt tgttcgtcgc cgtggcctgt ccggccgctg ccgaaccact ccaggccagg
     8761 gcaccgggcg ccgctgaaat cgacccgatc gaggatgcgg agcctgaaga gcccgaggaa
     8821 gaagcccccg cccaggcggc cgccgcgagc gcgaccccgg acgcgaagat tatccgcgag
     8881 gttcgcttca ccggcctgct gaggaccgat cgcgacaccg tgcttcgccg cgtcaagtca
     8941 ggcgtgcttt cggaactgga tccggcccgg gtttcacgcg atatcaaggc cctttacaag
     9001 accggtgcct tcgaaacgat ccaggctcgc gcgattccgg aagaagacgg cgtgattctg
     9061 gtgttcgagg tgaaggaata cccggccgtt gcggtcgtca ggacggaagg caacaagaag
     9121 atcgacaacg acaaacttaa ggaaaagatc gtcgtgcggg aatcgacgat cctcgacatg
     9181 aggaaggtcg agcggtcggt cgaggccatc aaggcgcact acgtcgagga agggtactat
     9241 cttgccgagg tttcctatcg gctggatccg aagcccgaca atgtcgtcga cgtcgttttt
     9301 gtcatccgcg aacactcgaa ggtcaaagtc aggtcgattg aattcctggg gaaccacaac
     9361 atcccggatc aggaccttaa gaaattcatg cagacccagg aaggcgacct gctttcaatc
     9421 gtttccagca agggactttt cgccaaggaa aaccttgaga tggacgttcg gcggctggaa
     9481 ttcttctata acacgaaggg ttacgccgag gtcaagatcg aggagccggt cgtgatgctg
     9541 tcccgcgacc gcaagcacat cgcgatcgcg ataacgattc atgaagggcc gcagtacaag
     9601 gtcggcaagg ttgacatcgg cggcgacatc atcttcccga aggaagacct gatgaagaag
     9661 ctggcgttga aggaaggcga catcttcaac gctgaaaacg tccagaagga cgcgatggga
     9721 ctcctgacga agtacaagga cgaaggttac gccttctgca atgtcggaaa tacgaccaac
     9781 atggatcaga agaacctgat cctggatttc acgtacatca tccagaaggg cgtcaaggcc
     9841 aggatcacgc gaatcgacat caccggcaac gaaggcacca gggactggac aatccgccgt
     9901 gaactgcgcg tctatgaagg cgacatgttc aacgagacgc agatgaaacg ttccgaggcc
     9961 aggattcgcc gcctgggctt cttcgaggaa gtcgagattg ttcccaggca gggcgacgga
    10021 ccggatcagg taatcctgac ggtgcgggtc aaggagcgcc agaccggcgc gttctcgatc
    10081 ggcgcgggct tcagttcggt cgagaacttc ctgttccagg cccagatcag caagcagaac
    10141 ttcctgggac gcggacagtc gctgcgactg gaagcaagcc tgtccagcat caggaagtac
    10201 ttcgtcttct cgttcgagga tccgaacttc ttcgactccg aatggacgtt cggattttcc
    10261 gcgtacaacc gcgactacat caattacacg tacgacgaaa actcgaccgg catgagcctg
    10321 acaatcggcc ggcgctttgc cgactatttc tcggtcgcgg cctcctacaa cctcgaaatg
    10381 gtggatgtca aggccggcgg acaggaggac ttct
//
LOCUS       DAOPPA010000050        10376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7323_length_10376_cov_4.451797, whole genome shotgun sequence.
ACCESSION   DAOPPA010000050 DAOPPA010000000
VERSION     DAOPPA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7323_length_10376_cov_4.451797"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="rpsC"
                     /locus_tag="PKH54_02595"
     CDS             <1..600
                     /gene="rpsC"
                     /locus_tag="PKH54_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOC98805.1"
                     /translation="RIPYIKNWNSRWYADKNYGQWTFEDVQIRKTVRKMMGAAGISSV
                     QIERTASKVKVYIYTARPGIAIGRKGENIEVLKRELAKLTTQEVVPYIQEVRKAELDA
                     QLVSESVAQQLERRVSFRRATKRAVSQAIKLGAKGIKIRCSGRLGGAEMSRVLWYREG
                     QVPLHTLRADIDFGFSEASTTFGKIGVKVWVYKGEVLKK"
     gene            655..1071
                     /gene="rplP"
                     /locus_tag="PKH54_02600"
     CDS             655..1071
                     /gene="rplP"
                     /locus_tag="PKH54_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOC98806.1"
                     /translation="MLSPARVKHRKQAKGKMRGVAYRGSDVSFGDYGLQALECGWLTA
                     RQIEAGRIAMTRHVKRGGQVWIRVFPDKPISKKPLETRMGRGKGSPEEWVAVIRPGRV
                     LYEIEGVDEATALEAFRLAGHKLPLSTKVLKRGEIR"
     gene            1068..1268
                     /gene="rpmC"
                     /locus_tag="PKH54_02605"
     CDS             1068..1268
                     /gene="rpmC"
                     /locus_tag="PKH54_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOC98807.1"
                     /translation="MKALKSEELHALTTEELIRRETQYRESLFKMRMKLATGQLSHVA
                     DVTATRRNLARILTEMNRRTGE"
     gene            1277..1546
                     /gene="rpsQ"
                     /locus_tag="PKH54_02610"
     CDS             1277..1546
                     /gene="rpsQ"
                     /locus_tag="PKH54_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOC98808.1"
                     /translation="MENAVHSRHKRQFTGTVASVAGQKSVVVVVERRFKHRTYHKYIT
                     TAARYMAHDENSVCKAGDQVLIEECRPMSARKRWRVVNKGETAAK"
     gene            1600..1968
                     /gene="rplN"
                     /locus_tag="PKH54_02615"
     CDS             1600..1968
                     /gene="rplN"
                     /locus_tag="PKH54_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOC98809.1"
                     /translation="MIQNETSLISADNSGAKRMKVIRILGGTGRRYATVGDIVVASVK
                     EAIPRAKVKKGDVVKCVIVRTAKEIGRVDGTYIRFDQNAAVVINPAGEPVGTRIFGPV
                     ARELRAKRFMKIVSLAPEVL"
     gene            1968..2291
                     /gene="rplX"
                     /locus_tag="PKH54_02620"
     CDS             1968..2291
                     /gene="rplX"
                     /locus_tag="PKH54_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOC98810.1"
                     /translation="MRKIRKGDQVQVLRGKDAGRKGKVLEIIGETERAVVENVNMVKK
                     HTKKTQKNLTGGIVEKAAPLPLSALAVLSKKDGKPVRVHFETRETAGKATKVRVASRT
                     GEVLD"
     gene            2328..2876
                     /gene="rplE"
                     /locus_tag="PKH54_02625"
     CDS             2328..2876
                     /gene="rplE"
                     /locus_tag="PKH54_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOC98811.1"
                     /translation="MTNIEKKYKEEVAPELQKQFGFGNPFQVPRLLKVTVNMGLGEAT
                     KNPKVIETSVAELTAITGQKPVVTRSRKSIAAFKVREGNAIGACVTLRGDRMFEFLDR
                     LMNVALPRVRDFRGVPTRAFDGHGNYTLGLREQIIFPEVDYDKIDKFKGMNITICTSA
                     ANDEEALVLLKLLGMPFRKATA"
     gene            2888..3073
                     /locus_tag="PKH54_02630"
     CDS             2888..3073
                     /locus_tag="PKH54_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOC98812.1"
                     /translation="MAKTSLRVKASRKPKFAVRAYNRCPICGRPHAYIRKFNMCRICF
                     RNAALRGDLPGVIKASW"
     gene            3086..3481
                     /gene="rpsH"
                     /locus_tag="PKH54_02635"
     CDS             3086..3481
                     /gene="rpsH"
                     /locus_tag="PKH54_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOC98813.1"
                     /translation="MITDPIADMATRIRNAILAGHESVEIPSSKLKAGIAKLLKDQGY
                     IDDFSVMDDRLQGILKVYLKYRGPKDNVITGIQRESKPGRRVYVGKEDIPSVRKGLGI
                     AIMSTPQGLMIGEEAKKRGIGGELLLSVW"
     gene            3559..4098
                     /gene="rplF"
                     /locus_tag="PKH54_02640"
     CDS             3559..4098
                     /gene="rplF"
                     /locus_tag="PKH54_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOC98814.1"
                     /translation="MSRIGKKPIPVPTGVEVKLSGKLVSVKGPLGTLSRPIPGAIVKI
                     EPGVVNVLRDEELPNGDAMHGLSRTLVANMVEGVSKGFSKTLVITGTGYKVEAGGRGL
                     ILSLGYSNPVDFELPASVKATVAEKNLKVTLTSIDKEVLGDVTAKIRALRPVEPYKGK
                     GVAYENEVVRRKAGKAGAK"
     gene            4115..4489
                     /gene="rplR"
                     /locus_tag="PKH54_02645"
     CDS             4115..4489
                     /gene="rplR"
                     /locus_tag="PKH54_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015650412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOC98815.1"
                     /translation="MEVRTAQFRKAARLARKTRIRRKISGTAECPRLVVFRSGRHIDC
                     QVIDDSRGHTLAAASTLSKEIREAVADLAKIEQAKKVGMLIADRCKAKGIESVVFDRN
                     GFLYHGRVAALADGAREGGLKF"
     gene            4520..5005
                     /gene="rpsE"
                     /locus_tag="PKH54_02650"
     CDS             4520..5005
                     /gene="rpsE"
                     /locus_tag="PKH54_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOC98816.1"
                     /translation="MQEKLDFSEHIVHLGRVAKVVKGGRRFSFAALVVIGNGEGLVGY
                     GLGKAQEVPEAIRKGSERARKNMIMVPVIDGTVPHEVIGHFGAGRVLIRPAAPGTGVI
                     AGGAVRAVLEAAGVRDVLTKVLGTRNPHNVVRATVAALRDLVSPEEAFEKRGLTGKET
                     A"
     gene            5005..5469
                     /gene="rplO"
                     /locus_tag="PKH54_02655"
     CDS             5005..5469
                     /gene="rplO"
                     /locus_tag="PKH54_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOC98817.1"
                     /translation="MANELSNLKPVPGSTHARKRVGRGTGSGLGKTSGRGHKGQGSRT
                     GKGQPRYFEGGQMPLQRRLPKRGFSPLDHKVFAVVNIGDLEVFPDNASVDTAALVAAG
                     LVRKIGHGVKILAKGDLKKKLNVVVHAFSVAAKEKIEKAGGTIRLVTEDAGA"
     gene            5489..6826
                     /gene="secY"
                     /locus_tag="PKH54_02660"
     CDS             5489..6826
                     /gene="secY"
                     /locus_tag="PKH54_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOC98818.1"
                     /translation="MANSLEGLGKVPELRKRLLYTLGMLLVYRIGIFVTVPGVDREIM
                     ENYIRYGQSGLLGLFDLFSGGAVSQMSIFALGVMPYISASIIFQLLAVVVPALERLKK
                     EGDAGTRKINQYTRYATVGLSVIQAISIAVWLESLSDNAGTENLLSSGGMLFRFLTVI
                     SLTTGTLLLMWMGEQITDRGIGNGISLIIFAGIVARLPDAVVQTFQLSTLGGGSLAPL
                     TLLLIAILIVVTIGFIVFFERAQRRIPVQYAKKVVGRKLYGGQTTFLPLKINMSGVIP
                     PIFASSILMFPATIANFFPGASWVSSMQNMLMPGGMLYNFMYVALILFFCYFYTAVTF
                     NPVDVADNLKKNGGFVPGIRAGKATADYIDKVLSRITFGGAVYISSVCVLPTILQGEF
                     NVPFYFGGTSILIVVGVAMDTASQIEAHLISRSYEGFAGAGGPRMRGRRSSIG"
     gene            6823..7491
                     /locus_tag="PKH54_02665"
     CDS             6823..7491
                     /locus_tag="PKH54_02665"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOC98819.1"
                     /translation="MTSARLGVILLGPPGAGKGSEAELIVERYGIPHISTGDMLRQQI
                     RSGTELGRLAATFIEKGLLVPDKVVVDMVSDRLGWSDCENGFLLDGFPRSVGQAEALH
                     ASGHTVTDVILLDVGDELIVRRITSRRSCPACGRVYNVVTLPSRDGVHCDSCQDVELI
                     VRADDGEATIRNRLREYQAMTAPLVEWYGARGLLKRVNGEGTVDEVFVRILEVISVGG
                     STEE"
     gene            7491..7709
                     /gene="infA"
                     /locus_tag="PKH54_02670"
     CDS             7491..7709
                     /gene="infA"
                     /locus_tag="PKH54_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOC98820.1"
                     /translation="MAKEEAIEVQGTVVEALPNAMFRVELENGVVILGHVCGKMRMHF
                     IRILPGDKVKVELSPYDLTRGRIVFRVR"
     gene            7719..7832
                     /gene="rpmJ"
                     /locus_tag="PKH54_02675"
     CDS             7719..7832
                     /gene="rpmJ"
                     /locus_tag="PKH54_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOC98821.1"
                     /translation="MKVKASVKKICDKCKIVKRQGIVRVICENPRHKQRQG"
     gene            7844..8212
                     /gene="rpsM"
                     /locus_tag="PKH54_02680"
     CDS             7844..8212
                     /gene="rpsM"
                     /locus_tag="PKH54_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOC98822.1"
                     /translation="MARIAGVDLPRKKQVGIALRYIYGLGPVLSMKVLGKAGVDPTTK
                     TDDLTEGEVARIREVLDSGEFKVEGDLRREISANIKVLMDMGCYRGLRHRRGLPVRGQ
                     RTHTNARTRKGRKGSTIKKK"
     gene            8227..8613
                     /gene="rpsK"
                     /locus_tag="PKH54_02685"
     CDS             8227..8613
                     /gene="rpsK"
                     /locus_tag="PKH54_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOC98823.1"
                     /translation="MAKAKGGKKKVRRDVPTGVVHISATFNNTIISITDPNGNVISWC
                     TAGRRGFKGSRKSTPFAAQTAAEDAARRAVESGMRSVHILVNGPGGGRESAVRAIAAA
                     GLRIAYIRDITPVPHNGCRPPKRRRV"
     gene            8628..9287
                     /gene="rpsD"
                     /locus_tag="PKH54_02690"
     CDS             8628..9287
                     /gene="rpsD"
                     /locus_tag="PKH54_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOC98824.1"
                     /translation="MARYRDSLCKVCRREGAKLFLKGERCYTEKCAFERRAYGPGQHG
                     QNRRKPTPYCTQLREKQKVRSIYGMLEKQFRLFFGRAERERGITGDNLLRKLETRLDS
                     VVARAGFAVNRNDARQLVRHGHVLINGRRVSIPSYSVKAGDVVEVASGSRQIKRVVDA
                     MAVAERRPACRWLEVDRGNFKARVASMPDPADFRSIVPGSEDLDGKAIREELIVELYS
                     K"
     gene            9331..10347
                     /locus_tag="PKH54_02695"
     CDS             9331..10347
                     /locus_tag="PKH54_02695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOC98825.1"
                     /translation="MHRNWRELIKPRSIVLDESSRTERYGKFVAEPFERGFGTTIGNS
                     LRRVLLSSIRGAAVTAVKIDGVLHEFSTIPEVVEDVSEICLNLKGVDLKMDGEESRKV
                     HAHFEGEGVITAGDVFKGPDVVTTNPGHVVCTMGPGAVVDIELTVKHGFGYNRAERNK
                     DPEAPVGTIPIDSIFTPIRRVNYRVSNARVGHMTDYDRLTLEVWTNGAVSPVDAVGIA
                     AKIIKEQVKVFINFDDRDDDAVVGIVGGGDDVGGGVVEAGASQEVLYRLVEELDLSVR
                     AQNCLQNAGIKYVGELVQRTEQEMLKTRNFGRKSLKEIKDVLADLDLSLGMRLDGFDP
                     DKRA"
BASE COUNT         2142 a         2872 c         3358 g         2004 t
ORIGIN      
        1 cgtatcccgt atatcaagaa ctggaattcc cgctggtacg cggacaagaa ttacggacag
       61 tggacttttg aagacgtcca gatccgcaag accgtccgca agatgatggg cgccgcgggc
      121 atttcctccg tgcagattga gcgtacggcc agcaaggtca aggtttacat ctacacggcg
      181 cgtcccggca tcgccatcgg acgcaagggc gagaacatcg aagtcctgaa gcgcgagctc
      241 gcgaagctca ccacccagga agtcgttccg tacatccagg aagtccgcaa ggccgaactg
      301 gacgcgcagc tcgtttccga gtcggtagcc cagcagcttg agcgtcgcgt cagcttccgt
      361 cgcgccacga agcgcgcggt cagccaggcg atcaagcttg gggccaaggg gatcaagatt
      421 cgctgctccg gtcgtcttgg cggcgccgag atgtccaggg ttctgtggta tcgcgaaggg
      481 caggttcccc ttcacaccct gcgtgccgac atcgacttcg gattctccga ggccagcacg
      541 acctttggaa agatcggagt caaggtgtgg gtctataagg gcgaagtact gaagaagtag
      601 ccggttcagc ggtttgtgtg ggagccccga acaggctacc ccgggaggta ggagatgctt
      661 tctccagcga gagtaaaaca ccggaagcag gcgaagggaa agatgcgcgg cgtcgcatac
      721 cgcggatccg acgtgtcctt cggtgattat ggtcttcagg cccttgagtg tggctggctt
      781 accgcccgcc agattgaagc aggccgtatt gcgatgaccc gtcatgtcaa gcgtggtggc
      841 caggtctgga tccgcgtgtt cccggacaag ccgatcagca agaagccgct tgaaacccgt
      901 atgggtcgtg gaaagggatc gcccgaggaa tgggtggccg tcatccgccc gggcagggtt
      961 ctttacgaaa ttgaaggcgt cgatgaggct acggctctgg aagctttccg cctggcgggc
     1021 cacaagcttc cactcagcac caaggttctg aagcgtggag agatcagatg aaagccctta
     1081 agagtgaaga actgcatgcg ctgaccacgg aagagctgat tcgcagggaa acgcagtacc
     1141 gcgagtcgct gttcaagatg cgcatgaagc tcgcgaccgg tcagctttcg cacgttgccg
     1201 acgttacggc cacgcgcagg aacctggccc gtatccttac cgaaatgaac cggcggaccg
     1261 gtgagtgagg gatgtaatgg aaaacgcagt tcacagcagg cacaagcggc agttcactgg
     1321 aacggttgcg agcgttgccg gccagaagtc ggtcgtggta gtcgttgaaa gacgattcaa
     1381 gcaccgtacg taccacaagt acatcacgac ggccgcccgt tacatggctc acgatgaaaa
     1441 cagcgtgtgc aaggcaggcg accaggttct tatcgaagaa tgcaggccga tgtcggccag
     1501 gaagcgctgg cgcgtcgtca ataagggcga aacggccgcg aagtgattgg attggttcga
     1561 cccttcgtgg tcggtgggta tagcaggtcg aggtagacca tgatccagaa tgaaacaagc
     1621 ctcatttcgg ctgacaactc aggcgccaag cgcatgaagg tcatccgtat ccttggcggt
     1681 accggcaggc ggtacgccac tgtcggcgac atcgtcgtcg ccagcgtcaa ggaagcgatc
     1741 ccgagggcga aggtcaagaa gggtgacgtc gttaagtgcg tcatcgtcag gaccgccaag
     1801 gagatcggac gtgttgacgg gacctacatc cgtttcgacc agaacgcggc ggtcgtaatc
     1861 aaccccgcag gcgagccggt tggaacccgt atcttcgggc cggtggccag ggagctgcgt
     1921 gcgaagcgct tcatgaagat cgtttcgctg gctccggagg tgctgtgatg aggaagattc
     1981 gcaagggtga tcaggtccag gtcctgcgcg gcaaggatgc cgggcgcaag ggaaaggtcc
     2041 tggagattat cggtgagacc gagagggccg ttgttgagaa cgtcaacatg gtcaagaagc
     2101 acaccaagaa gacgcagaag aacctgaccg gcggcattgt cgagaaggca gccccgctcc
     2161 cgctctctgc cctggcggtg ttgagcaaga aggacggcaa gcccgtgcgg gttcattttg
     2221 agactcgcga gaccgccggt aaggcaacca aggtcagggt tgcgagccgt accggtgagg
     2281 tcctggatta gtcgcgccgg aaggcgccga tacaggtggc ggaagatatg acgaacatcg
     2341 agaagaagta caaagaagaa gttgcccccg agctgcagaa gcagttcggt ttcggcaacc
     2401 ccttccaggt tccgcggttg ctcaaggtaa ccgtcaacat gggcctggga gaggcgacca
     2461 agaaccccaa ggtcatcgag acctcggttg ccgaactgac tgccatcacc ggccagaagc
     2521 cggtcgtgac cagatcccgc aagtccattg cggcgttcaa ggtcagggaa ggcaacgcga
     2581 tcggcgcgtg cgtgaccctt cgtggcgatc gcatgttcga gttccttgat cggctgatga
     2641 atgttgcgct tccgcgcgtc cgggacttcc gtggcgtgcc gacccgcgcc ttcgacggcc
     2701 acgggaatta cacgctcggg cttcgtgagc agatcatttt ccccgaagtc gattacgaca
     2761 agatcgacaa gttcaaggga atgaacatca cgatctgcac cagcgcggcc aacgacgagg
     2821 aagccctggt actgctgaag ttgctcggaa tgcccttccg caaggcaacc gcataggagg
     2881 atcagccgtg gcaaagacat ctttgagagt taaggcctcc cgcaagccga agttcgcggt
     2941 cagggcttac aaccgttgcc cgatttgcgg tcgtccacat gcgtacatcc gcaagttcaa
     3001 catgtgccgt atctgtttcc ggaacgcagc ccttcgcggg gatctgcccg gtgttatcaa
     3061 ggcgagctgg taggagaggc gaattatgat taccgatccc attgcggaca tggcaacgcg
     3121 catcaggaac gctatcctcg ccggtcacga atcggtggag attccgtcct cgaagctcaa
     3181 ggccgggatt gcgaagctgc ttaaggacca ggggtacatc gatgactttt cggtcatgga
     3241 tgatcgcctg cagggcatcc tgaaggtcta tctgaagtac aggggtccga aggacaacgt
     3301 gattaccgga atccagcgcg aaagcaagcc cggtcgtcgc gtctatgttg gcaaggaaga
     3361 catccccagc gtccgcaagg gtctgggaat cgcgatcatg tcaacgcccc agggcctgat
     3421 gattggcgaa gaagcgaaga agcgcggaat cggcggcgag ctgctgctgt ccgtctggtg
     3481 atgtgaattc gacccggtcg tgccgggtca tggaaggccg gacgcccagg cgcgccggcc
     3541 gggaattcgg aggacagcat gtccagaata ggtaagaagc cgattccggt tccgaccggg
     3601 gtcgaggtaa agctgagcgg caagctcgtt tccgtgaagg gccccctggg aaccctctcg
     3661 aggccgatcc ccggggccat cgtcaagatc gagccgggcg tcgtgaacgt tcttcgcgac
     3721 gaggaactgc cgaacggcga cgcgatgcac ggtctgtcaa ggaccctggt cgcgaacatg
     3781 gtcgaagggg tcagcaaggg cttttcgaag accctcgtga ttaccggtac cggttacaag
     3841 gttgaggctg gcggtcgcgg cctgatcctg agcctgggat actcgaatcc agtggatttc
     3901 gagcttccgg ccagtgtcaa ggctaccgtc gccgaaaaga atcttaaggt caccctgacc
     3961 agcatcgaca aggaagtgct gggcgacgtg accgcgaaga ttcgtgctct gcggcctgtc
     4021 gaaccctata agggcaaggg cgtggcctat gagaatgaag ttgtaaggcg caaggctggc
     4081 aaggcaggcg ccaagtaagc ggtggaggct taacgtggaa gttcgcacgg cacaattcag
     4141 gaaggccgcc cggctggctc gcaagacccg catacgccgg aagatcagcg gtacggcgga
     4201 atgcccccgt ctggtcgtat tccggagcgg aaggcacatc gactgccagg tgatcgacga
     4261 ctcccgggga cacaccctgg cggccgcatc gacgctttca aaggaaatcc gcgaggcggt
     4321 cgccgacctg gccaagattg aacaggccaa gaaggtcgga atgcttatcg ctgatcgttg
     4381 caaggcaaag ggaatcgaat ccgttgtctt tgaccgcaac ggctttttgt atcacggacg
     4441 cgttgccgct ctggccgatg gtgccaggga gggtggcctg aaattctgat gaggtgacgt
     4501 cttgagcgcc gaagaacaga tccaggagaa gctcgatttc tccgaacaca tcgttcatct
     4561 tggccgtgtc gccaaggtcg tcaagggcgg tcgcaggttc tccttcgcag ccctggtggt
     4621 catcggcaac ggcgaaggcc tggttggtta cggacttgga aaggctcagg aagttcccga
     4681 ggccattcgc aaaggttccg agcgggcccg caagaacatg atcatggtcc cggtcatcga
     4741 cggaaccgtg ccgcatgaag tgatcggtca cttcggcgcc ggcagggttc tgattcgtcc
     4801 ggcggccccc ggtaccggcg tcatcgccgg cggtgcggtg cgcgcggtcc ttgaagcggc
     4861 cggagtcagg gacgtcctga ccaaggtcct tggaacccgc aatccccaca acgtggtccg
     4921 ggcaaccgtg gccgcgctcc gggaccttgt ttcacccgag gaggcctttg agaagagggg
     4981 ccttacgggc aaggagacag cgtaatggct aacgaacttt caaatctcaa gcctgttcct
     5041 ggttcgactc acgcccgcaa gcgggttggt cgcggcaccg gttcaggact tggcaagaca
     5101 tccggtcgtg gtcacaaggg ccagggttcc cgcaccggca agggtcagcc ccgatacttc
     5161 gagggtggcc agatgccgct gcagcgccgt ctgcccaagc gcggattttc cccgctggat
     5221 cacaaggtgt tcgccgttgt caacatcggc gaccttgaag tgttcccgga caacgccagc
     5281 gtcgataccg cggccctggt tgcggccgga ctggttcgca agatcggcca cggcgtgaag
     5341 attctcgcca agggcgatct caagaagaag ctcaacgtgg tggttcacgc cttctcggtc
     5401 gcggcgaagg aaaagatcga aaaggccggt gggacgatca ggctcgtgac cgaagacgcc
     5461 ggtgcctgag ccggtggagg gatgacccgt ggccaatagc cttgaaggac tcggaaaggt
     5521 tccggagctt cgcaagcggc tgctctatac gctgggcatg ctgcttgtgt accgcatcgg
     5581 tatcttcgtt acggttcccg gggttgaccg ggagattatg gagaactaca tccggtacgg
     5641 ccagtccggt ctgcttggac tgttcgacct gttctccggt ggcgccgttt cccagatgtc
     5701 gatcttcgcg ctgggcgtca tgccttacat cagcgcgtcc atcatcttcc agctcctggc
     5761 ggtcgtagtg ccggcactcg agcgtctgaa gaaggaaggg gacgccggca ccaggaagat
     5821 caatcagtac acccggtacg cgacggttgg actctccgtc attcaggcga tcagcatcgc
     5881 agtctggctc gagtccctct ccgacaacgc cggcaccgaa aacctgctgt cctcgggcgg
     5941 catgctgttc cggttcctga ctgtaatctc cctgactacc ggtacccttc tgctgatgtg
     6001 gatgggtgaa cagatcaccg accgcggcat cggcaacgga atttctctga ttatcttcgc
     6061 cggtatcgtc gcgcgtctgc cggacgccgt cgtccagacg ttccagcttt caacgctggg
     6121 cggtggcagc cttgccccgc tgacgcttct gctgattgca atcctgattg tggtgacaat
     6181 cggcttcatc gttttcttcg aaagggccca gaggcgcata ccggttcagt acgcgaagaa
     6241 ggtcgtcggt cgcaagcttt acggcgggca gacgacgttc ctgccgctga agatcaatat
     6301 gtccggcgtc attccgccga tcttcgccag ttccatcctg atgttcccgg cgacgatcgc
     6361 gaacttcttc ccgggcgcgt cctgggtgag ctcgatgcag aacatgctga tgccgggcgg
     6421 aatgctttac aacttcatgt acgtcgcgct gatcctgttc ttctgctact tctacaccgc
     6481 ggttaccttt aacccggttg acgtggccga caaccttaag aagaacggcg gcttcgtccc
     6541 cggcatccgt gccggcaagg cgacggccga ctacattgac aaggtgcttt ccaggatcac
     6601 gttcggcggc gcggtctata tatcgtccgt ctgcgttctg cccacgattc tgcagggtga
     6661 attcaacgtg ccgttctact tcggcggcac gtccatcctg atcgtcgtcg gtgtcgcgat
     6721 ggatacggcc tcacagatcg aggcccacct gatcagccgc agttatgaag gctttgccgg
     6781 cgccggcggt ccccgcatgc gcggcagacg gagcagtatt ggatgacgtc cgctcgactt
     6841 ggtgttatac ttctcggccc gccgggcgca gggaagggaa gcgaggcgga actgatcgtt
     6901 gaacggtatg gaattcctca catatccacc ggggatatgt tgaggcagca gatccggtca
     6961 ggcaccgaac ttgggcggct ggctgcgacg ttcattgaaa agggtttgct tgttcctgac
     7021 aaggtggtgg ttgacatggt cagcgaccgc ctcggatgga gcgattgcga aaacggcttc
     7081 ctgcttgacg gctttccccg ttccgtcggc caggccgagg cgctgcacgc gtccggccac
     7141 acggtcacgg acgtgattct tctggatgtc ggcgacgagc tgattgtccg caggatcacc
     7201 agcagacggt cgtgtccggc ttgcggcagg gtatataacg tggtgacgct tccatcccgg
     7261 gacggcgttc actgcgattc ctgccaggac gtcgaactga tcgtccgagc tgacgacggc
     7321 gaggcgacca tcaggaaccg gcttcgcgag taccaggcga tgacggcccc gctggtcgag
     7381 tggtacggtg ccagggggct gttgaagagg gtcaacggcg agggaacggt ggacgaggtg
     7441 ttcgtccgga tcctcgaagt catttccgtt ggcggttcga cggaggagtg atggcgaagg
     7501 aagaggccat agaggtccag gggactgtcg ttgaggcgct cccgaacgcc atgttccggg
     7561 ttgagctcga gaatggtgtt gtgatcctgg ggcatgtatg cggaaagatg cggatgcatt
     7621 tcatcaggat ccttccgggt gacaaggtga aggtcgagct ttccccgtac gacctgactc
     7681 ggggacggat cgttttcagg gtgcgatagg aggcgaccat gaaggtcaag gcttcggtaa
     7741 agaagatctg cgacaagtgc aagatcgtaa aacggcaggg tattgtccgc gtgatctgtg
     7801 agaacccgcg gcacaagcag cggcagggat aattggaggc gtcatggctc gtatagcagg
     7861 tgttgacctc cccagaaaga agcaggtggg aatcgctctc cgttacattt acggactcgg
     7921 tcccgttctg tcgatgaagg ttctgggcaa ggcgggtgtg gatccgacga cgaagacgga
     7981 tgatcttacg gaaggcgaag tcgcccgtat ccgcgaggtc ctggattctg gcgaattcaa
     8041 ggttgaaggt gatcttcgcc gtgaaatttc cgcgaatatc aaggtcctga tggacatggg
     8101 ctgctaccgt ggccttcgtc accgcagggg cttgccggtt cgcggtcaga gaacccatac
     8161 caatgcgcgt acacgcaagg gtagaaaggg ctccacaatc aagaagaagt agggaggatc
     8221 cgccagatgg ccaaggctaa gggtggtaag aagaaagttc gcagggacgt gccgaccggg
     8281 gtggttcaca tctcggcgac gttcaacaac acaatcattt cgatcactga tcctaacggg
     8341 aacgtgattt cgtggtgcac ggccggtcgc aggggtttca agggctcccg caaatcgacg
     8401 ccgttcgccg cccagacagc ggccgaggac gcggcaaggc gcgcggtcga atcgggcatg
     8461 cggtcggtgc acatcctggt caatggacct ggtggcggac gtgagtcggc ggtcagggcc
     8521 atagcggccg ccggtctgcg tatcgcgtac attcgcgaca ttacgccggt tccgcacaac
     8581 ggttgccgtc cgccgaagcg tcggcgcgtc tgattggagg aatacccgtg gcacggtaca
     8641 gggatagtct ttgcaaggtc tgccgccgcg agggagccaa gctcttcctc aagggcgagc
     8701 gctgctacac agaaaagtgc gcctttgagc gcagggctta cggtcccggc cagcatggcc
     8761 agaatcgccg caagccgacg ccgtactgca cccagcttcg cgagaagcag aaggttcgca
     8821 gcatctacgg catgcttgaa aagcagttca ggctgttttt cggccgtgcc gagcgcgagc
     8881 gcggcatcac gggcgacaat ctgctccgca agcttgaaac caggcttgac agcgtcgtgg
     8941 cccgcgccgg attcgcggtc aacaggaacg acgcgaggca gcttgtccgc catggtcacg
     9001 tcctgattaa cggccgcagg gtttcgattc catcgtattc cgtgaaggcc ggcgatgtcg
     9061 tcgaagtcgc ttccggaagt cgtcagatta agcgcgtggt cgatgcgatg gcggtggccg
     9121 agcgtcgtcc ggcgtgccgc tggctggaag tcgatcgcgg caatttcaag gcgcgcgtcg
     9181 cgtccatgcc cgatccggcc gatttcaggt cgatcgtccc cggttccgag gacctcgacg
     9241 gcaaggcgat tcgcgaagaa ctgatcgtgg agttgtactc caagtaatgg acggtcggga
     9301 tatttcccga acctcccgga ggcgctcatt atgcatagaa actggcgtga gctgattaag
     9361 ccccgttcga tagtcctgga tgaatcctcg cgtaccgaac gctacggcaa gttcgttgcc
     9421 gagcccttcg agcgcgggtt cgggaccacg atcggcaatt ccctgcgcag ggttctgctg
     9481 tcctccatca ggggcgcggc ggtgaccgcg gtcaagattg acggcgtcct tcacgagttt
     9541 tcgacgatcc ccgaggttgt cgaggacgtg tcggagatct gcctgaacct gaagggcgtt
     9601 gatctgaaga tggacggcga ggagtcccgc aaggtgcatg cgcacttcga gggcgagggc
     9661 gtcatcacgg ccggcgacgt gttcaagggg ccggacgtgg tcaccacgaa tccgggccac
     9721 gttgtctgca cgatgggacc gggcgccgtg gtcgatatcg aactgaccgt gaagcacggc
     9781 ttcggataca accgcgccga gcgcaacaag gatcctgaag caccggtcgg cacgattccg
     9841 attgattcga ttttcacccc gatccgcagg gtcaactaca gggtttccaa cgcccgtgtc
     9901 ggccatatga ccgattacga ccgtctgacg cttgaagtct ggacgaacgg tgcggtctcc
     9961 cccgttgacg cggtcggcat cgcggcgaag atcatcaagg aacaggtcaa ggttttcatt
    10021 aacttcgacg acagggacga cgacgccgtc gtcggaatcg tgggcggagg cgatgacgtc
    10081 ggtggtggcg tggttgaggc cggcgcttcc caggaagtcc tttaccggct ggtcgaggaa
    10141 ctggatcttt ccgttcgcgc gcagaactgt ctgcagaacg cgggaatcaa gtacgtcggc
    10201 gaactggtgc agcgaaccga gcaggagatg ctgaagacca ggaatttcgg aaggaagtcc
    10261 ctgaaggaaa tcaaggacgt cctggcggat ctggatcttt cgcttggtat gcgcctggac
    10321 ggcttcgatc ccgacaagag ggcctgacag cggaaggttt gatttttggc ggccgg
//
LOCUS       DAOPPA010000051        10249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7441_length_10249_cov_4.027467, whole genome shotgun sequence.
ACCESSION   DAOPPA010000051 DAOPPA010000000
VERSION     DAOPPA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7441_length_10249_cov_4.027467"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..483)
                     /locus_tag="PKH54_02700"
     CDS             complement(76..483)
                     /locus_tag="PKH54_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98826.1"
                     /translation="MPRGLIPAILVAMLTSTACAPAFTGRWDGSGEIGEAGYFSFSLD
                     FVKGPGSGEFRTDKSDVVAGPVCQVVRRDRHVEFVIASRSGSADCASLSYPLTFVGDF
                     GQDVVTGSVLERKADGGERLVGVFRAFRVRKEK"
     gene            625..2073
                     /locus_tag="PKH54_02705"
     CDS             625..2073
                     /locus_tag="PKH54_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOC98827.1"
                     /translation="MGTGIRDLEPRVVFEIFDDLVAIPRGSKKEEAAREYCLAWARRH
                     GFDVREGAAGSIAISVPATPGHESAPIIVIQGHLDIVWEKTPETAIDFETDPIPAFVE
                     GEFIRTRGTTLGADNGIGVAAGMAAAIDQSCVHGPLELLLTVDEETGMGGAFGLEPDL
                     LRGRVMLNLDSEEEGTMYVGCAGGGDTTITMKTTAIPRPAGTMAFRIGVSGLRGGHSG
                     LEIHAGRGNSIRLLARMATVLDPIHPPLFCTISGGTKRNAIPRDAEACVFIPESDADH
                     FKSAVAKLQQTVKIELGKSDPGVNLSVTPVECDCQAIAESPAIWKMLLALPHGPVTMS
                     QAIVGLVETSSNMAQVTTLEGSVQILCNSRSMVGSAMEAVRESIRAVAGLAGADCELI
                     NSYPGWRPDMDSKVLKTARALWKERTGVDPRVTAIHAGLECGVIGEKYPGMDMISFGP
                     NITGAHSPEETLSIPSTRIFYDFLKALLERLA"
     gene            complement(2125..3063)
                     /locus_tag="PKH54_02710"
     CDS             complement(2125..3063)
                     /locus_tag="PKH54_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98828.1"
                     /translation="MESLSLVFEGGFKLSGGILGFDHPGAGLSFLSSAEVPGAWRSRK
                     PLCTETTASLLRNLRREFRALVLPYSRPTGIGSIIVTLFPAAPLPGAAAILVEHGGRR
                     LLYCAGLPDRSVHLPACDIVAAVVAPEPGLPSADFRERLTRSVTTALSAGMTPVIRSN
                     SFGGAIAVSQALSLAGVPCRQHENGFRHNAVWRATGFESCLCARLGSRIPAGSAVLLP
                     LHLSQGAVESRIVAPWRICVARPGEDGGPAPCDELIRLHAGLEPVSMVELVSRTSPAE
                     VVVWGQGAEPCRAMLAGSGVEVRTVDGTRQCALDLS"
     gene            complement(3075..4847)
                     /locus_tag="PKH54_02715"
     CDS             complement(3075..4847)
                     /locus_tag="PKH54_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98829.1"
                     /translation="MFGQIRLRPDHTESSRIRNLLRSLSLRDSFPLESMDRDDGDSSN
                     TFRGRLVWLSWIRLAMLVALAGSTIGFSLGENVALDGDARGFLMWFTIVCIVPGSLYF
                     PLILAVRSMVALRSVAVIQIIQDSAFAAVMVTTTGGSTSGFTFFFPLMIIIAGLLLQK
                     TGALIGILISSLFLLIISMWEIGMLVPPPMIGTMLTTGRFEDVFYAYAINVVAFIVVG
                     VLSNFLMAQIRKSDVERETYRIDLADLRILHETIISSLDTGLVTVSLEGTVVHLNRAA
                     AQLLRLDARAAVGQPIQSILPELNRPLEDIEGEVDIVRKSGDEAPSYFLVVVKPLLSS
                     RWMMVGRLIRIEDVTGIRQMEAQRKEDERLATIGKLSAVVAHEIRNPLAAISASAQMV
                     AMSPQVGEEDRQALEIVVKETDHLNEWITDLLDYARPKRGVLSEFDLSEMVLHQAGLA
                     GSFEGAVGVSIITDIEPDVIIKGDSQRFGRVVMNIMKNAVEAMNGIGTIKVGLKTGQS
                     GSGESSNETPPPPVAVLTIFNDGPAIPPEELGRIFDTFYTTKARGTGLGLSTVAQICA
                     DYGAKLSVTSDEHEGTEFRVEIPL"
     gene            complement(4871..6088)
                     /locus_tag="PKH54_02720"
     CDS             complement(4871..6088)
                     /locus_tag="PKH54_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC98830.1"
                     /translation="MAKFSWEATSAEGIRKKGVIDAASPDEARKRLRSMNLEPVSVSR
                     SLSSIEITIPRLSGIPMKTLVVFTRQLATMIDAGLPLVQCLDILGTQEPDLEFKKIIM
                     GVKAHVETGGTLADGMKKYPKAFDTLFVSLVQAGEVGGILDTILNRLAAYVEKNQKTK
                     QKVQAALKYPTFVSVAAVVIVGFMLWKIVPQFAGMFKSLGGAELPALTAMVVAASEWF
                     VNHVIIILLIATGAVVGFTLFKKSKFGISFLDKVALRLPVIGDLIRKSAVARFTRTLG
                     TLISSGVPIMEALDVVARSAGNVHIEAAIMHTRAKVAEGHNIAGPLGETKIFPSMVVQ
                     MVGVGESTGALDVMLTKIADFYDDEVDVAVDSLTSLMEPLLMIVIGGSVGVVLISMYL
                     PIFGMADNLSRKQ"
     gene            complement(6109..7428)
                     /locus_tag="PKH54_02725"
     CDS             complement(6109..7428)
                     /locus_tag="PKH54_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOC98831.1"
                     /translation="MMEEGRRSNTQNMQAVRAGDSGMARNPTTQAVRTGESGMARNPT
                     VQAQPQSAQPQSAPSQSAPVQGSPTQIVQTNAGPAGQGGQGVPGLDINQLLQVMVEKN
                     ASDLHITVGSPPQLRIDGSLYPIKVPPLIPADTQRLCFSILNDAQRQKFEDMHEVDLS
                     FQWRTKSRFRANFFMQRGSIAGAIRQIPQKILSMEELGLPPALAQVLDRPRGLVLVTG
                     PTGSGKSTSLASMVDTINQRRRGHIITIEDPIEFLHRHKNCIVNQREIGADTADFHGA
                     LRYIMRQDPDVVLVGEIRDRETMETALTLSETGHLVFSTLHTNGAVQTLHRVMDLFPA
                     DQQERVRTQLSFVIEGIMSQQLLRRVDGGRVVAVELMIPNAAIRNLIREGKAHQIYSQ
                     MQMGQAKFGMQTMNQSLLRLHQEGYITRDDCLMTAGDLEEMIAMLNG"
     gene            complement(7449..9173)
                     /gene="pilB"
                     /locus_tag="PKH54_02730"
     CDS             complement(7449..9173)
                     /gene="pilB"
                     /locus_tag="PKH54_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOC98832.1"
                     /translation="MPVKVDSGAAMGEILLREKLVNVDQLHEAKQVQGDGENLGYTLA
                     RLGYLEESQLINFLSRQYGVPSINLDEVEVIDDVISLVPREVAERHCLMPIARQGSTI
                     IVAMTDPGNIYAMDDLKFLTGLNVESVVASETAIKTAIERYYGESKAGADLDGIIREM
                     QEEEDDVSLVDEADAVDVTDLTKATEEAPVVRLVNMILVDAVKRQASDIHVESYEKDF
                     RVRYRMDGVLYEIMRPPMRFKNAITSRLKIMAELDIAERRLPQDGRIRMVMGHKKEVD
                     FRVSVLPCLFGEKIVLRILDKGALKLSLDAIGFEAKPLAHFREAINQPWGMVLVTGPT
                     GSGKSTTLYSALSELNMPDTNISTAEDPVEMNISGINQVHVREDIGLTFASALRSFMR
                     QDPDIIMVGEIRDFETAETAVKAALTGHLVLSTLHTNDAPSTINRLLNMGIEPFLLTA
                     TLVLICAQRLVRRICPECKEPFPVDKSVLIDIGFSPEQAEGLVPMKGKGCKNCNGTGY
                     RGRSGLYEIMPMFDELKEYVLQGYSAIELKREAMKMGMLTLRQAGLAKVAAGTATIEE
                     VLRVTRAD"
     gene            complement(9403..>10249)
                     /locus_tag="PKH54_02735"
     CDS             complement(9403..>10249)
                     /locus_tag="PKH54_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC98833.1"
                     /translation="LSAKIGDELVAALVEGADKTDDADIAAQRERVEGLRAKLADDKS
                     PEARRLLVLADYLVHKSVWILGGDGWAYDIGYGGLDHVLAQGRNVNILVLDTEVYSNT
                     GGQASKSTPLGAAAKFAMAGKGTAKKDLAMIAMTYGNVYVAKVAIGAKDAQTVKAFKE
                     AESWPGTSIIIAYSHCIAHGYELIDGPDHQATAVSTGIWPLFRWDPRRLAEGLSPLQL
                     DSAAPVGDVGDFVKNETRFRMVQAANPDRFEKLMDMARKEVATRFAMYDQISKMDFST
                     QKDSE"
BASE COUNT         2134 a         3146 c         2926 g         2043 t
ORIGIN      
        1 ccggaccaac gtgaacgaac tgctcggcac cggtctcgtc ctggaatttc agaagagcca
       61 cgtcaacacc tccggttact tttccttcct gaccctgaac gcccggaaaa ccccgacgag
      121 acgctcgcca ccatcggctt tccgttcaag cacgcttccg gtaacgacat cctgtccaaa
      181 atcaccgaca aaagtcagtg gataggaaag gcttgcacaa tccgccgagc cggaccggga
      241 cgcgatgaca aattcaacgt gccggtcgcg gcgaaccacc tgacataccg gcccggcaac
      301 gacatctgac ttatctgtcc ggaattcccc ggagcctggc ccctttacaa aatccaggct
      361 gaaactgaaa tacccggcct cgccgatttc accggaaccg tcccatcggc cggtgaaggc
      421 gggggcgcag gcggttgaag tcagcattgc gacgagtatc gcgggtatca agcctctggg
      481 catcggttcc tccgcagaat caggttcatg ccggattatt catcaattgc ttcgggaata
      541 gtactttcac tgcgtgtttc aggcaaggtg gacaagcatc gcggacatga tatcctgtgt
      601 tcgcaattgc tttggaggct gccaatggga accggaatcc gtgatcttga gccaagggtt
      661 gtttttgaaa tcttcgacga tctggttgcg ataccgcgcg gttcgaaaaa ggaagaagcc
      721 gcccgtgaat actgcctggc atgggcccgc aggcacggct tcgacgtcag ggaaggcgcg
      781 gccggcagca tcgccatatc ggttccagcc acgccgggac acgaaagcgc gccgatcatc
      841 gtcatccagg gacatctgga cattgtctgg gaaaagacgc cggaaaccgc gatcgacttc
      901 gagacggatc ccattccggc ctttgttgaa ggggaattca tacgcacccg aggcaccacg
      961 cttggcgccg acaacggcat tggcgtcgcc gccggaatgg ccgccgcaat cgaccagtca
     1021 tgcgtgcacg ggccacttga actgctgctg accgttgacg aggagaccgg catgggcggc
     1081 gctttcgggc ttgaacccga tcttcttcga ggcagggtga tgctgaacct ggactcggaa
     1141 gaagaaggca ccatgtacgt tggttgcgcc ggcggcggcg acacgacgat taccatgaag
     1201 acaacggcaa taccccgtcc ggccggaacc atggcattca ggatcggggt ttcaggcctg
     1261 cgtgggggcc actccggcct tgagattcat gccggccgtg gcaactcgat tcggctgctt
     1321 gccaggatgg caacagtgct cgatccaatc catccgccgc ttttctgcac gatttcgggt
     1381 ggcaccaagc gaaacgcgat cccacgggat gccgaggcct gcgtcttcat cccggaatca
     1441 gacgccgatc acttcaaatc ggccgtggcg aagcttcaac agacagtgaa gatcgaactt
     1501 gggaagtcgg atcccggcgt caacctgtct gtcaccccgg ttgaatgcga ttgccaggct
     1561 atcgcggaat cgcccgcaat ctggaagatg ctgctggcgc tgccccatgg cccggtaacg
     1621 atgagccagg ccatcgttgg cctggttgaa acgtcttcga acatggccca ggtcacgacg
     1681 ctggaaggct ctgtccagat cctgtgcaac agccggtcga tggttggatc tgcaatggaa
     1741 gcggtccgtg aatcgattcg cgcggtcgcc ggcctggccg gtgccgactg cgaactgatc
     1801 aattcctatc ccggctggcg tcccgacatg gattcaaaag tcctgaagac ggccagggcg
     1861 ctgtggaagg aaagaaccgg cgtcgatccc cgggtcaccg cgattcacgc cggtctcgag
     1921 tgcggcgtca tcggcgagaa ataccctgga atggacatga tttcttttgg tccgaacatc
     1981 accggggcac attcccccga ggaaacgctt tcgattccat ccaccaggat tttctatgat
     2041 ttcctgaagg ccctgctcga aagactcgcc tgaagacggc ccacggttcg accgacagcc
     2101 ccgtcccggg gactcactga aaggtcatga cagatccagc gcgcactgcc tggttccatc
     2161 gacagtccgc acttcgacac cgctgccggc aagcatggcc cggcatggct cggctccctg
     2221 gccccagacc acgacttccg ccggggatgt cctggaaaca agctcaacca tggaaacggg
     2281 ttcaaggccc gcatgaaggc ggatcagttc gtcgcagggc gcgggcccgc cgtcctcgcc
     2341 cggtctggca acgcatatcc tccagggagc gacgatcctg gactcgacgg ctccttgcga
     2401 aagatgaagc ggcagaagga cggccgaacc cgccggaatg cgcgaaccaa gacgggcgca
     2461 gaggcaggat tcgaatccgg tcgcccgcca caccgcgtta tgccggaaac cgttttcatg
     2521 ctggcggcag ggaactccgg caagtgaaag cgcctggctg acggcgatgg ctccaccaaa
     2581 ggagttgctc ctgatgaccg gggtcatgcc ggccgacagc gccgtagtga ccgacctggt
     2641 aagcctttcc cgaaagtcgg cggaaggcag gccgggctcc ggggccacaa ctgcagccac
     2701 aatgtcacag gccggaaggt gaaccgaacg atccggaagt ccggcgcagt acagaagccg
     2761 ccggccgcca tgctcgacaa ggattgccgc ggcccccggc aacggggccg ccgggaacag
     2821 tgtcacgatg attgatccga tgccggtcgg cctcgaataa ggcagaacca gcgcgcggaa
     2881 ctcacgtctc aggtttcgca acaacgaagc cgtcgtttcg gtacacagcg gcttccttga
     2941 cctccacgcc ccgggcacct cggcggacga caggaaggaa aggccggccc cggggtgatc
     3001 aaatccaagg attccgccgg ataacttgaa gccgccttcg aataccagtg agagtgattc
     3061 catcagaccg tccgtcacag gggtatttcc accctgaatt cggtaccttc atgctcgtcg
     3121 ctggtcaccg agagtttcgc cccgtaatcg gcgcatatct gcgcgacggt ggaaaggcca
     3181 agccctgttc cacgagcctt ggttgtgtag aaggtatcaa atatccggcc cagttcctct
     3241 ggcgggattg cagggccgtc attgaatatg gtcagcacgg cgaccggggg cggcggagtc
     3301 tcatttgaag actctccgga gccggactgg ccggtcttca acccgacctt gatggttcct
     3361 atcccgttca tggcctcaac cgcgttcttc atgatgttca tgacgacgcg tccaaacctc
     3421 tgggaatccc ccttgataat cacgtccggt tcgatgtcgg tgattatcga gacgccaacg
     3481 gcaccctcga acgaaccggc aaggccggcc tggtgaagaa ccatctccga gagatcgaac
     3541 tccgacagga cccccctctt cggcctggca taatccagca gatccgtgat ccactcgtta
     3601 agatgatccg tctccttaac cacaatctcc agggcctggc gatcttcctc tcccacctga
     3661 ggcgacatgg caaccatctg ggccgatgcc gaaatcgccg ccagcggatt gcggatttcg
     3721 tgcgcgacca cggccgacag cttgcctatt gtggccaggc gttcatcttc cttcctctgg
     3781 gcctccatct ggcgaatgcc ggttacgtcc tcgattctga tcaagcggcc caccatcatc
     3841 caccgacttg acagcagggg cttaacgacg accaggaaat acgaaggcgc ctcatccccg
     3901 gactttcgaa ctatatcgac ctcgccctcg atatcttcaa gcggacgatt gagttccggc
     3961 aggatggact gaatcggctg gccgacggcg gcccttgcat caagacgcag aagctgtgcc
     4021 gccgccctgt tgagatgaac cacggtgcct tccagggaaa ccgtcaccag cccggtgtca
     4081 agcgaagaaa tgatggtctc gtgcaggatg cgcagatcgg ccaggtcaat ccgataggtt
     4141 tcgcgctcga catcggactt gcgaatctgg gccatcaaaa aattggaaag caccccgacg
     4201 acgatgaacg ccacaacatt gatcgcgtac gcgtaaaaga catcctcgaa ccgtcccgtc
     4261 gtcagcatcg tcccaatcat cgggggcgga acgagcattc cgatttccca catggaaatg
     4321 atcagcagga acagtgacga gataaggatg cctatcaacg ctccggtctt ctgaagcaac
     4381 agtccggcaa tgatgatcat aaggggaaag aagaacgtga agccggatgt cgaaccgcct
     4441 gtcgttgtca ccatgacggc ggcgaaggca ctgtcctgga tgatctgaat caccgcgaca
     4501 ctgcgcaggg cgaccatcga ccggacggca aggatcagcg gaaagtacag ggatcctgga
     4561 acgatgcaga caatggtgaa ccacatcagg aaaccgcggg catctccatc aagcgcgaca
     4621 ttctcgccaa gcgagaagcc aatcgtggat cccgccagcg cgacaagcat tgccagccgg
     4681 atccacgaca gccacaccag gcgtcccctg aaggtgttgc tgctgtcgcc gtcgtcccga
     4741 tccatcgact caagcggaaa gctgtcccga agggaaagag acctgagcag gttcctgatt
     4801 cgtgacgatt ccgtatggtc ggggcgtagt cgaatctggc cgaacattcg tgccaccggg
     4861 cgcggggcgc ctattgcttt ctggaaagat tgtccgccat gccgaagatc ggcaggtaca
     4921 tcgaaatgag cacgacgccg accgatccgc cgatgacaat catcagcaat ggttccatga
     4981 gtgacgtaag cgaatcgacg gcgacatcga cctcgtcgtc gtagaagtcg gcgatcttgg
     5041 taagcatgac gtccagtgcg ccggtcgatt ccccgacgcc gaccatctgg acgaccatcg
     5101 acggaaagat cttcgtttca cccagcggac cggcaatatt gtgaccttca gccaccttgg
     5161 cgcgggtgtg catgattgcg gcttcgatat ggacgtttcc ggcagatctt gcaacgacat
     5221 caagcgcttc catgattgga acaccggacg atatcagggt gccgagcgtc ctcgtgaatc
     5281 gggcgaccgc cgacttcctg atcaggtcgc cgattactgg caggcgcagc gcgaccttgt
     5341 ccagaaagga aatgccgaat ttggacttct tgaacagagt gaatccgacc accgcacctg
     5401 tcgcaatcag caggatgatg atgacgtgat tgacgaacca ttccgatgcc gcgacaacca
     5461 tggccgtcaa cgccggaagc tcggcgccgc caaggctctt gaacatgccg gcgaactggg
     5521 gaacgatctt ccagagcatg aagccgacga ttacgacggc ggccacggaa acgaatgtcg
     5581 ggtacttcag cgccgcctga accttctgct tcgtcttctg gttcttttcc acatatgcgg
     5641 caagcctgtt caagatggta tccagaatac cgccgacttc gccggcctgg acaagcgaaa
     5701 cgaaaagcgt atcgaaggcc ttcggatact tcttcatgcc gtcggccagc gttccgccgg
     5761 tttcgacgtg tgccttgacg cccatgatga tcttcttgaa ttccaggtcg ggctcctggg
     5821 ttccaaggat gtccaggcat tgaaccaggg gaaggcccgc gtcaatcatc gtcgcaagct
     5881 gacgggtgaa gacgacaagc gtcttcatcg ggatgccgct gagcctggga atcgtaatct
     5941 cgattgacga caggctgcgg ctgaccgata ccggctccag gttcatcgat ctaaggcgct
     6001 tcctggcctc gtcggggctg gcggcatcga ttacgccctt cttcctgatt ccttccgccg
     6061 atgtcgcttc ccagctgaac ttggccattc aaacctccaa tgcggacgtc atccgttaag
     6121 cattgcaatc atctcttcca ggtcacccgc cgtcatcagg caatcatcgc gggtgatgta
     6181 tccttcctgg tgaagacgca gcaacgactg gttcatcgtc tgcatgccga acttcgcctg
     6241 tcccatctgc atctgtgagt atatctgatg agccttgcct tcccgaataa gattacgaat
     6301 ggcggcattc ggaatcatca attcgaccgc aaccacgcga ccgccatcaa cacgccgcag
     6361 caactgctgg gacatgattc cctcgataac gaacgaaagc tgggttcgaa cgcgttcctg
     6421 ctgatcggcc gggaaaaggt ccatcacgcg gtgcagcgtc tggacggcgc cgttggtatg
     6481 cagggtcgag aaaaccaggt ggcccgtttc cgaaagcgtc aacgcggttt ccatggtctc
     6541 ccggtcgcgg atttcgccga caaggacgac gtccggatcc tgtcgcatga tataacgcaa
     6601 cgcaccgtgg aagtccgccg tgtccgcgcc gatttcgcgc tggttgacaa tgcagttctt
     6661 gtggcggtga aggaactcga tcggatcctc gatcgtgatg atatgaccgc ggcgcctctg
     6721 gttgatggta tcaaccatcg aagccagcga tgtcgatttg ccggaaccgg tcgggccggt
     6781 taccagcaca aggccgcgcg gtcgatccag aacctgcgcc agggccggag gcagcccgag
     6841 ttcctccatc gacaggatct tctgggggat ctgtcggatt gcaccggcga tactgccgcg
     6901 ctgcatgaag aaattcgccc ggaaacgact cttggttcgc cactggaagg acagatcgac
     6961 ttcgtgcatg tcctcgaatt tctggcgctg cgcgtcgttc aggatgctga agcagaggcg
     7021 ctgcgtgtcg gccgggatca gcgggggaac cttgatcgga taaagcgacc cgtcaatcct
     7081 caactgcggc ggcgaaccga cggtgatgtg aagatcggac gcattctttt caaccatcac
     7141 ctgaagcaac tggttgatgt caagaccggg aacgccctga ccaccctgcc ctgccggacc
     7201 ggcgtttgtc tgaactatct gggtcggcga gccctgaacc ggcgcactct gtgacggggc
     7261 actctgtggc tgcgcactct gtggctgcgc ctgaacggtt ggattcctgg ccatcccgga
     7321 ttcgcccgtc ctgaccgcct gcgtggtggg attcctggcc atcccggagt ctcccgccct
     7381 gaccgcctgc atattctgcg tattggatct tctgccttct tccatcatcc cataaccccg
     7441 atcgagcatc agtccgcgcg ggtcacgcgc aggacttcct cgatcgtcgc cgttcccgcc
     7501 gccaccttgg ccaggccggc ctgacgcaac gtcagcatcc ccatcttcat ggcttcacgc
     7561 ttcagttcaa tcgccgaata tccctgcagg acgtattcct tcaattcgtc gaacatcggc
     7621 atgatctcgt aaagaccgga ccgaccgcgg taaccggtcc cgttgcaatt cttgcagccc
     7681 ttgcccttca tcggaaccag gccctctgcc tgctccggcg aaaagccgat gtcgatgagg
     7741 acgctcttat cgaccgggaa aggttccttg cattccgggc aaatcctgcg taccagtcgc
     7801 tgggcacaaa tcaggacaag cgtggccgtc agcaggaacg gttcgatacc catgttcagg
     7861 agtcggttga tggtcgatgg cgcatcgttc gtatgcaggg tcgaaagcac caggtggcct
     7921 gtcaacgcgg ccttgaccgc ggtttcggcc gtttcgaagt ctcgaatctc cccgaccatg
     7981 atgatgtcgg ggtcctgccg catgaaggat ctcaacgcgg atgcaaacgt cagcccgatg
     8041 tcttcacgca cgtgaacctg attgatgccc gaaatgttca tttcaaccgg gtcttcggcc
     8101 gtggagatat tcgtgtcggg catgttcaat tcggaaagcg ccgaatacag ggtcgtcgat
     8161 tttccggatc ccgtcggacc ggtaaccagc accattcccc acggctgatt gatggcctcg
     8221 cggaaatggg ccagcggctt ggcctcgaaa ccgatcgcat ccagggacag cttcagggcg
     8281 cccttatcca ggattcgaag aacaatcttc tcgccgaaga ggcacggcag gaccgacacg
     8341 cggaaatcga cttccttctt gtgtcccatc accatcctga tacggccgtc ctggggcaga
     8401 cgcctttcgg cgatatccag ttcggccatg atcttcagac gggacgtgat cgcgttcttg
     8461 aaccgcatcg gcggacgcat tatttcgtaa agaaccccgt ccatcctgta cctgacgcgg
     8521 aagtcctttt cgtaactctc gacgtggata tcggaagcct ggcgcttgac cgcgtcaacc
     8581 aggatcatgt tgacgagcct gacaaccggt gcttcctcgg tcgccttggt caggtcggtg
     8641 acatcgacgg cgtcggcttc atcaaccagg ctgacgtcgt cctcctcctc ctgcatctca
     8701 cggatgatcc catccagatc cgcccctgcc ttcgactctc cgtaatacct ctcgatggcc
     8761 gtcttaatcg cggtctcgga ggcaaccacc gattccacgt tcagacctgt aagaaacttc
     8821 aggtcgtcca tcgcgtagat gttgccgggg tcggtcatcg ccacgatgat ggtggaaccc
     8881 tgcctcgcga ttggcatcag gcaatgacgc tccgccacct ccctgggcac cagggagatc
     8941 acgtcgtcga ttacctcgac ctcgtccaga ttgatcgaag gaaccccgta ctggcgggaa
     9001 aggaagttga taagctggct ttcttcaaga tatccaagcc ttgcaagcgt atatcccaga
     9061 ttttcgccgt caccctgaac ctgcttggcc tcgtggagct gatccacgtt gaccagtttc
     9121 tcgcgcagca ggatttcacc catcgcggcg ccgctgtcaa ccttgaccgg catgtactcg
     9181 cggctgcgcg ggggctggga gggcgagtcg ggaatcggcg gtggcatcat gggcttgcca
     9241 ttctttccgt tgtcaatcgg catctgaagt cctcgtttca ccaggtaagc ttggatgctg
     9301 gacatagtcc ggcgccgtgt attctaaccc tcgaccggaa tcaaggcaat aaatggggat
     9361 gtggccggac gggaactgca ggacggcggg aatcaggagc ccttactcgg aatccttctg
     9421 ggtcgagaag tccatcttcg aaatctggtc gtacatggca aatcgcgtcg cgacctcctt
     9481 gcgggccata tccatcagct tctcgaagcg atccgggttc gcggcctgca ccatccggaa
     9541 gcgggtctca ttcttcacga agtccccgac atcgccgacc ggcgcggcag aatcaagctg
     9601 caacggactc agaccctcgg ccaggcgcct gggatcccag cggaacagcg gccagatgcc
     9661 ggtgctgact gcggtggcct gatggtccgg cccgtcgatc agctcgtaac cgtgggcgat
     9721 gcagtgcgag taagcgatga tgatggacgt tcccggccag gattctgctt ccttgaacgc
     9781 cttgacggtc tgggcatcct tcgcgccgat tgcgaccttc gccacataga cgttgccata
     9841 ggtcatggcg atcattgcca gatccttctt ggcggtaccc tttccagcca tcgcgaattt
     9901 ggcggcagcc cccagcgggg ttgacttgga cgcctgtccg ccggtgttgc tgtaaacctc
     9961 ggtatccaga accaggatat tgacgttccg gccctgggcc aggacgtgat ccagtccgcc
    10021 gtaaccgatg tcataggccc atccgtcacc gcccaggatc cacacgctct tgtgaaccag
    10081 gtagtccgcc agaaccagca ggcggcgggc ttccggtgac ttgtcgtcgg cgagcttcgc
    10141 gcgaaggcct tcaacccgtt cacgctgcgc ggcgatgtcc gcatcatccg tcttgtcggc
    10201 gccctcgacc agcgcggcaa caagttcgtc gccgatcttc gccgacagg
//
LOCUS       DAOPPA010000052        10247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7445_length_10247_cov_3.687304, whole genome shotgun sequence.
ACCESSION   DAOPPA010000052 DAOPPA010000000
VERSION     DAOPPA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_10247_cov_3.687304"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKH54_02740"
     CDS             <1..555
                     /locus_tag="PKH54_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98834.1"
                     /translation="ASTDLTVPEDSTGAGDMDGEIGANLRIRCETGDPECPEGFQCAD
                     DSMNGRFCKPESDCNLSGISSIADLLEGLDIFDITTPIFIKISTTVSMGSQTCSMLKC
                     ETDNPCCNQCWAGLVIDESDVPIQLTGSDGMIGCKGTECDFDQHCVPMSPGEKYIIWG
                     TAQMFGDGPQLKVEGFCPASVIDA"
     gene            602..1351
                     /locus_tag="PKH54_02745"
     CDS             602..1351
                     /locus_tag="PKH54_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC98835.1"
                     /translation="MPEIKLESDQPRATALVTGATVRVGLEIALALARSGRDLVLHCR
                     RHDQRAEDAAAAVAATGARAFIVEADLTDRQAVERMFRNIGQMAGGLDILVNSAAVFG
                     FSPPDTLSVADFDHFIDTNLKAPYMCCILARKLMKPGASIVNIADVAAERPFRNHVPY
                     CISKAGIVMLTRSLAKAWAPDIRVNAISPGTVLFRDDEDEATRAAVRRRIPMARIGTP
                     EDVAKAVIFLCDNAAHTTGAILQVDGGRSLD"
     gene            complement(1380..2399)
                     /gene="ruvB"
                     /locus_tag="PKH54_02750"
     CDS             complement(1380..2399)
                     /gene="ruvB"
                     /locus_tag="PKH54_02750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOC98836.1"
                     /translation="MADTTGRFIAPEKTEEEVMEASLRPRTLDEFVGQDALKAKLKVF
                     VSAARQRGEALDHILLCGPPGLGKTTLAHILARELGVEIRQTSGPALEKKGDLAGILT
                     NLAHRDVLFIDEVHRLTTVVEENLYPAMEDFNFDVIIGEGAHARSIKLKLKKFTLIGA
                     TTRSGLLTSPMRDRFGIVERLEHYPVKDLQTIVTRSAKILGVQIEPEAASELAARSRG
                     TPRIANRLLRRVRDFAQVEGDGRISLEVTRSAMERLDVDQFGLDSSDRRYLYCIIDKF
                     GGGPVGVETLCAALSEERDTIEEVFEPYLIQEGFIQRTPRGRVATDRARRHLHRPVNA
                     SGELL"
     gene            complement(2405..3001)
                     /gene="ruvA"
                     /locus_tag="PKH54_02755"
     CDS             complement(2405..3001)
                     /gene="ruvA"
                     /locus_tag="PKH54_02755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOC98837.1"
                     /translation="MIGRLKGKLVSCESGIAVVDVGGVGFEVNVDAQTALDLGMPGDN
                     VVLVIRTVVREDAITLFGFFDDTTRLVFDLLRNVTGIGPKMALAIMGGLPVHVMVQAV
                     REKDIRLLATISGVGKKTAERLVMELGERFMSLPITAPAVSGQVAPTLVSDLRSALAN
                     LGYNPRDVDAAIRTLDPVEPDADLESMVMAALGWLSSR"
     gene            complement(3020..3580)
                     /gene="ruvC"
                     /locus_tag="PKH54_02760"
     CDS             complement(3020..3580)
                     /gene="ruvC"
                     /locus_tag="PKH54_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOC98838.1"
                     /translation="MPRILGLDPGSRKTGYGIIEGTWGNLRCVDYGVLRLDTGKPAPS
                     DEISALLGEDPSQSVASMTRRMVELYSRVSEVIRSFGIDEVALERVFVSKNADSALKL
                     GHARGVLLLAVGNAGLPVNEYSPAQVKKCVAGSGRAEKSQMMSIVPAILGMDRNPPED
                     AADALAIAMTHAMLGGERAMYLDSIR"
     gene            complement(3589..4338)
                     /locus_tag="PKH54_02765"
     CDS             complement(3589..4338)
                     /locus_tag="PKH54_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC98839.1"
                     /translation="MSGHSKWATIKHKKGKLDAQRGKIFTKLIKEITIAARMGGGDPE
                     GNPRLRQAILTARGQNMPFDNIDRAVKKGTGELEGVIYEEVTYEGYGPGGAAIIVDCQ
                     TDNKNRCVSEIRRVFTKAGGNMASQNAVAWMFDQKGLIEVDHAGVEEDALVEAAIEAG
                     ADDVQDQEELFEVTTAPSDLMAVADALKSAGFNVTSAKFAKIPQNMIRLTGKEAEQML
                     RFVETLEDLDDTQNVWTNFDVDESELESLMG"
     gene            complement(4521..5675)
                     /gene="metK"
                     /locus_tag="PKH54_02770"
     CDS             complement(4521..5675)
                     /gene="metK"
                     /locus_tag="PKH54_02770"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOC98840.1"
                     /translation="MTTDTMLFSSESVTEGHPDKVADQISDAILDSIIAQDRNCHVAC
                     ETLVTTGMALVAGEITTDCWVDIPAVVRKTISEIGYNDPELGFEAKTCAVITSIDKQS
                     PDIAMGVNRDSPDEQGAGDQGMMFGYASSETPELMPMPIVLAHRLTSRLADVRKDGTL
                     GFLRPDGKSQVSVRYADGRPVSVQAVVVSTQHTPDVTLADIRDAVISHVVEKVIPAEL
                     MTADTKIFVNPTGRFVIGGPMGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRS
                     AAYYARYAAKNIVAAGLAERCELQVAYAIGVAEPVSILVQTFGTSRIPEARISELIRR
                     HFDFRPGRLIRELDLLTPVYRQTASYGHFGRDGFRWEMTDKVDALRADAF"
     gene            6019..6786
                     /gene="dapB"
                     /locus_tag="PKH54_02775"
     CDS             6019..6786
                     /gene="dapB"
                     /locus_tag="PKH54_02775"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOC98841.1"
                     /translation="MIFAINGARGRMGRMLVEVLSDPTVSSTHQVGGLVERAGHPDTG
                     GIVRVAGTDLVLVDDPEALPRDIDVGLDFSAAGSGTAFARAMARRHAALLVGVTGLPA
                     EDLLALREIGKKTPVLVAANTALGVLCLSELAATARRMLGNNYDVEVFEMHHRHKKDA
                     PSGTALTLARRLESTGLHAAADRNGQRQDGEIGISAARGGEVVGVHTIYFLGPNDQLE
                     LTHRANSRKAFAEGAVKMAALLRAKAPGYYEINDLLR"
     gene            complement(6767..7852)
                     /locus_tag="PKH54_02780"
     CDS             complement(6767..7852)
                     /locus_tag="PKH54_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC98842.1"
                     /translation="MLEFNPQDYGRPWLEGVAVVVNLVGDARFNVTGSSPEEVAEAAR
                     VVQRHDGLHRVDIVERMLARDGQPLARRDGADWIAGVEDAMCALADAITSAGAGISPF
                     SVAITGIPVTRNEPDGKADVEAVSALVRDGLGLSVSSCWPSSCGVADLAAVCRSGIIV
                     ALPNGLRVAAAIAARTGARVVQAPIPLGFDNTSRFVGIVASACGRRERGAEFVNAELG
                     RIVPRFEWVVPHSLLNRRIGLVGDASMIQAAAGFLQEVGCVPCRGAVTGSGDCAVDLS
                     AIFDDDPGEPMALTIGNSDFTKFAATTDEPFLEAWFPCQDHHSFFPKPQFGFDGAAAF
                     LQDIVNRMAFFEIMSSWKNTVINEEDR"
     gene            complement(7839..8657)
                     /locus_tag="PKH54_02785"
     CDS             complement(7839..8657)
                     /locus_tag="PKH54_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC98843.1"
                     /translation="MIALYGKGGIGKSTVASNISAILASRGLKVLHVGCDPKADSTRN
                     LLTPDSGLTTVIDHLAGSIESADEIVFKGRLGLDCIECGGPEPGVGCGGRGVARMFEV
                     LDELELIEQGNYDAAIFDVLGDVVCGGFAAPLRQGRADLVYIVISEGVMALYAANNIA
                     KAMKRFATNGIGLGGLILNSTGPQTNYDGMRQFAQRIGTRIVAEIPRHPLIARAEIAG
                     LPIVMADPDSDVVAIFQGLADVMMGDAPESHPVPTPMTDAEFDAFIKETLGAGI"
     gene            8839..9213
                     /locus_tag="PKH54_02790"
     CDS             8839..9213
                     /locus_tag="PKH54_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98844.1"
                     /translation="MNPTAFQIFCGYYLGLDSEFGAKFFNVHSLAAHYGIDAGTLQEL
                     MAAHGMVPENMRHIDFNVAKAHGIAQELTFTASREDVIRHARDSFDAFVQAARKMDPA
                     KVFEDIDYDDVWEDEKDNRGNR"
     gene            9284..9364
                     /locus_tag="PKH54_02795"
     tRNA            9284..9364
                     /locus_tag="PKH54_02795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9318..9320,aa:Leu,seq:tag)
     gene            complement(9412..>10247)
                     /locus_tag="PKH54_02800"
     CDS             complement(9412..>10247)
                     /locus_tag="PKH54_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98845.1"
                     /translation="GYPTDYGIATQSFTHELGTSFGHSGMSYGYRSATFHYPESGWTY
                     SMTCNTYPAPWMEFDTELAAILLDPATSPAASCDVSGNFDLNPAIPEIQAKFRARINT
                     GDGEYVPTGFGTAMLVTGGATESDVTRETMFGRNVFVTTLDDGTVDIRFRGQAFDNPD
                     AFWRDTHILVNPGYLRAISAGDRISTHDVDLYIEDIHGTDDPETYSRRCVVGVVNRIA
                     PSRIGLCDEIDIADEALLRGWISVPVTRKPADIETWLAVSGRPACQCRDAGGNWSACP
                     D"
BASE COUNT         2143 a         3150 c         2925 g         2029 t
ORIGIN      
        1 gcttcgacgg atctaacggt tccagaagac tcgaccggcg caggcgacat ggatggcgaa
       61 atcggtgcaa atctccggat tcgctgcgag actggcgacc ctgaatgtcc ggaaggtttc
      121 cagtgcgccg acgactcgat gaacggtcgt ttctgcaagc ccgaaagcga ttgcaacctt
      181 tccggcatca gcagtatcgc ggatcttctt gaagggcttg atatctttga tataacgaca
      241 ccgattttca tcaaaatatc gaccaccgtg tcaatgggaa gccagacctg ctcaatgctt
      301 aaatgcgaaa ccgacaaccc gtgctgcaat caatgctggg ccggtcttgt aatcgatgaa
      361 tcggacgtcc cgatccagct tacaggctcg gacggcatga ttggatgcaa gggaaccgaa
      421 tgcgatttcg atcagcactg cgttccgatg tcaccgggtg aaaaatacat catctgggga
      481 acggcccaga tgtttggtga cggtccccag ctcaaggttg aaggcttctg cccggcttcc
      541 gttatcgacg cctgaacggc tcgggttata ctccagttcg ccacacgagt gcggtgctgc
      601 catgccggaa atcaaacttg aatcggatca gccgcgggcg actgccctgg tgaccggggc
      661 aacggtaagg gtcggacttg aaatcgccct ggccctggcc cgttctggtc gggatctggt
      721 gcttcactgc cgaaggcatg accagcgcgc cgaggatgcc gcggcggccg tcgccgccac
      781 tggggcccgg gctttcatag tcgaggcaga tttgaccgac cggcaggcgg tcgagaggat
      841 gttccggaac atcggccaga tggccggcgg gcttgacatc ctggtcaatt cagccgcggt
      901 gttcggcttt tcgccaccgg acaccctttc cgtcgcggat ttcgaccatt tcatcgatac
      961 caacctgaag gcaccctaca tgtgttgcat ccttgccaga aagctgatga agcccggggc
     1021 aagcattgtc aacatcgccg acgtcgccgc cgagcgaccg tttcgcaacc atgtgcctta
     1081 ctgcatctcc aaggccggca tcgtcatgct gaccaggtcg ctcgccaagg catgggcgcc
     1141 cgatatccgg gtcaacgcga tatctcccgg caccgtgctt ttccgggacg acgaggacga
     1201 ggcaacgcgc gcggcagtca ggcgacgcat accgatggcg cggatcggca ccccggaaga
     1261 cgtcgcgaag gcagtaatct ttctgtgcga caacgccgcc cacacaacgg gggcaatact
     1321 gcaggtggat ggcggcaggt ctctggactg aaaggccgat ccatgcccga tattccgggc
     1381 tacagcaact cacccgaagc atttacggga cgatgcaggt gacggcgggc acggtcggtc
     1441 gcgacgcgtc cccttggcgt gcgctggatg aagccttcct gaatcagata gggctcgaag
     1501 acctcctcga tggtgtcacg ctcctcggac agggccgcgc aaagggtttc cacgccgaca
     1561 ggaccgccgc cgaacttgtc gatgatgcaa taaagataac ggcgatccga cgaatccagc
     1621 ccgaactgat cgacgtcaag gcgttccatg gccgatctgg tgacttccag ggaaatccgg
     1681 ccatctccct cgacctgcgc gaagtcgcgc acacggcgca gcagccggtt ggcaatcctg
     1741 ggcgtacccc tggatctggc cgccaactcc gacgcggcct cgggctcgat ctggacacca
     1801 aggatcttcg ccgacctggt gacgatggtc tgcaagtcct tgaccggata gtgttcaaga
     1861 cgttctacaa ttccgaacct gtcgcgcatc ggcgacgtaa gcaaaccgga tctggtcgtg
     1921 gcgccgataa gcgtgaattt cttcaacttc agcttgattg acctggcgtg cgcaccctcc
     1981 ccgattatca cgtcgaagtt gaaatcctcc atcgcgggat acaggttttc ctcgacaacg
     2041 gtggtaagac gatgaacttc atcgataaac agcacatccc tgtgtgcaag gttggtgaga
     2101 atcccggcaa ggtcaccctt cttttccagg gcggggccgg atgtctgcct gatttcgacg
     2161 ccaagttccc tggccaggat atgggccagc gtggtcttgc caagccccgg cgggccacag
     2221 agaagaatgt gatcaagcgc ctcgccgcgc tgcctggcgg cggagacgaa gaccttcagc
     2281 ttggctttca gggcgtcctg gccgacaaac tcgtcaagcg tccttggccg gagagacgcc
     2341 tccatcactt cttcttcagt cttttccggc gcaataaaac gacctgtcgt gtccgccatc
     2401 tgtttcacct cgaactcagc caacccaggg ccgccattac catggattcc aggtctgcat
     2461 ccggctcgac cggatcaagg gttcttatcg ccgcatcgac atcccgtgga ttgtatccaa
     2521 gattggcaag cgccgatcga agatcgctga ccagcgtcgg cgcgacctgg ccggacaccg
     2581 cgggggccgt gattggcagg ctcatgaagc gttcgccgag ttccatgacc agacgctcgg
     2641 cggtcttctt tccaacgccg cttatggttg caagaagcct gatatccttc tcgcgcacgg
     2701 cctggaccat gacgtgaacc ggcaacccgc ccatgatggc caaagccatc ttcggtccga
     2761 tgccagtaac gttgcgcaac aggtcaaaga ccagccgcgt ggtatcgtcg aagaaaccga
     2821 acagcgtgat cgcgtcctcg cgcacgacgg tccggatcac cagcacgacg ttgtccccgg
     2881 gcattccaag atcaagcgcg gtctgggcat cgacgttgac ttcaaacccg accccgccga
     2941 catcgaccac agcgatcccg gactcgcagg atacaagttt ccccttcagc ctgcctatca
     3001 tctgaacctc cgaccggtat tatcggatcg aatcaagata catggccctt tcgccgccaa
     3061 gcatagcatg tgtcatggca atcgccaggg cgtccgcggc gtcttcgggc ggatttctgt
     3121 ccattcccag tatcgcgggc acgatggaca tcatctggga cttctcggcc cgtccactgc
     3181 ctgcgacgca cttcttcacc tgcgcggggg aatactcatt gacgggaaga ccggcgttgc
     3241 caaccgccag cagcagcacc ccgcgggcat gacccagctt caaggccgag tcggcgttct
     3301 tcgaaacgaa cacccgctca agagccactt catcgattcc aaagctgcgg atgacttctg
     3361 acacgcggga gtaaagctcg accatcctcc tggtcatcga tgccaccgac tgtgaaggat
     3421 cttcgccgag aagcgcggat atttcatcag atggcgccgg cttgccggtg tcaaggcgca
     3481 gcacgccgta atcgacgcag cgcaggtttc cccacgtgcc ttcgatgatg ccgtaaccgg
     3541 tcttgcggct tccggggtca agtccgagaa ttcggggcat atccccaatc aacccatcag
     3601 ggattcaagt tcagattcat cgacgtcgaa gttggtccag acgttctggg tatcgtccag
     3661 atcctcgagc gtctcgacga agcgcagcat ctgctcggct tccttgccgg tcaggcggat
     3721 catgttctgg ggaatcttgg cgaacttggc cgatgtaacg ttgaagccgg ctgacttcag
     3781 ggcatccgcc acggccatca ggtcggacgg cgccgtcgtt acctcgaaca gttcctcctg
     3841 atcctgaacg tcgtcggcac cagcctcgat tgcggcctcg accagcgcgt cttcctcgac
     3901 gccggcgtgg tcgacttcga tcaggccctt ctggtcgaac atccaggcga ccgcgttctg
     3961 gctggccatg ttgccgccgg ccttcgtgaa cacgcgacgg atttcggaaa cgcagcggtt
     4021 cttgttgtcg gtctggcagt caacgatgat tgcggcgcca ccgggtccgt aaccttcgta
     4081 cgtgacttct tcataaatca cgccttccag ttcgccggta cccttcttca ccgccctgtc
     4141 gatattgtcg aacggcatgt tctgcccgcg ggccgtcagg atcgcctgcc tcagcctggg
     4201 gttgccttcc ggatcaccgc cgcccatcct tgcggcaatg gtgatttcct tgatgagctt
     4261 cgtgaagatc ttgccgcgct gggcgtcaag cttacctttc ttgtgcttaa tggttgccca
     4321 tttactatga cctgacattc cattgcctca catctgagag gggccaaaaa aaaagcaacg
     4381 gacttttacc acaaaatcaa gccatttaca atccgccaac acgcagaaag tggttcgaca
     4441 agtgtaaata actgagggaa aaggaatacc gggatgcaag cgattggaag ggcacaaaaa
     4501 gcgcaaccat ttgaatttaa tcagaatgca tcggcacgaa gggcgtcgac cttatcggtc
     4561 atctcccaac ggaaaccgtc acgcccgaaa tggccgtaag aagcggtctg gcgatatacc
     4621 ggggtcagaa ggtccagttc ccggatcagc ctgccaggcc tgaaatcaaa atgcctccgg
     4681 atcagttccg aaatgcgggc ctccgggatc cgtgacgttc cgaaggtctg gacaaggata
     4741 cttaccggtt cggcgacgcc gattgcatag gcgacctgaa gttcgcatct ttccgcaagc
     4801 ccggccgcca cgatgttctt tgcagcatac ctggcgtagt aggccgcgct gcgatcgacc
     4861 ttgctcggat ccttgccgga aaacgcgccg ccgccatgcc tgcccatccc gccgtacgta
     4921 tcgacgatga tcttgcgacc ggtcagcccg cagtcgccca tcggcccgcc gatgacgaag
     4981 cggccggtcg gattgacgaa tatcttcgtg tccgcggtca tcagttcggc tggaatcacc
     5041 ttttcgacga cgtgggaaat caccgcgtcc cggatatcgg caagcgtgac gtccggcgtg
     5101 tgctgggttg agacgaccac ggcctgaacc gacaccggac ggccatcggc atatcggacg
     5161 gaaacctggg actttccgtc gggacgaaga aatccaagtg ttccgtcctt cctgacgtcg
     5221 gcaagtcggg acgtcagtcg gtgggccagc acaatcggca tcggcatcag ttccggggtc
     5281 tcggacgatg cgtaaccgaa catcatgccc tgatccccgg cgccctgttc atccggcgaa
     5341 tccctgttga cgcccatggc gatatcaggg gactgcttgt cgatgctggt gatgacggcg
     5401 cacgtctttg cctcgaatcc aagttcaggg tcgttatacc caatctcgga tatcgtcttt
     5461 cgcaccaccg ccgggatatc aacccagcaa tcggtcgtga tttccccggc aaccagggcc
     5521 attcccgtgg taaccagagt ctcgcacgca acgtgacaat tcctgtcctg cgcgatgatg
     5581 gaatccagaa tggcgtcgga aatctggtcg gcaaccttgt ccggatggcc ctcggtcacg
     5641 gattccgaag aaaacagcat ggtatcggtt gtcatgttca aacctcgaaa atcaaatcga
     5701 attcaggctt ccaatgatcc ggtgggccgg gcgcccggcg ccggatttgt agcgatattt
     5761 gacaccaaaa tcaaccaaaa ttgtccgagt ttgatggaaa cgtacatcga accgggacgt
     5821 tatgaatcca accgggataa acctgccgga tgtcacacga cgggaaatcc cagggtcacc
     5881 accttgaggc ccgcgtccgc aacccaagtc gaacagaagc catcttgcgg gttaatcgtt
     5941 tgtaaagtaa cttcaaatcc gatgggacat cagtcccggc ttgctgaatc caaactggac
     6001 acacaagggg tttttcgaat gattttcgca atcaacggcg ctcgaggacg aatgggccga
     6061 atgcttgtcg aagtgctttc agacccgacc gtttcttcga cccatcaggt gggcgggctg
     6121 gttgaaaggg ccggacaccc cgacactggc ggcattgtgc gggtcgcggg aacggatctt
     6181 gtgcttgtcg atgatccgga ggcccttcca cgcgatatcg acgtcggact ggatttcagc
     6241 gccgccgggt cgggtacggc attcgccagg gcgatggcca gacgccatgc cgcgctgctt
     6301 gtcggcgtca ccggccttcc cgccgaggat cttcttgcct tgcgcgagat cggcaaaaag
     6361 actccggttc tggtcgcggc aaacaccgct ctcggggttc tgtgcctttc cgagcttgcc
     6421 gcgacggcaa ggcgaatgct tggaaacaat tacgacgtcg aagtgtttga gatgcaccac
     6481 aggcacaaga aggacgcccc gtccggaact gcgctcacgc ttgcccgaag gcttgaatcg
     6541 accgggcttc acgcggccgc cgacaggaac gggcagcgcc aggatggcga aatcggcata
     6601 tccgcggctc gtggggggga agtggtcggg gttcacacga tttacttcct gggcccgaac
     6661 gaccagcttg aactgaccca ccgggcgaac tcaagaaagg cttttgccga gggcgcggtg
     6721 aaaatggcgg ccctgcttcg cgcgaaggcc ccgggttact acgaaatcaa cgatcttctt
     6781 cgttgatcac cgtattcttc caactcgaca ttatctcgaa gaatgccatc cggttcacga
     6841 tgtcctgaag gaaggctgcc gcaccatcaa agccaaactg cggcttcgga aagaacgagt
     6901 ggtgatcctg acatggaaac catgcctcca ggaagggctc gtcggtcgtg gctgcaaact
     6961 tcgtgaagtc cgaattcccg atcgtcaggg ccatcggctc gcccggatcg tcatcgaata
     7021 tcgccgacag atcgacggca cagtcgccgg aaccggtcac cgcgccgcga cacgggacgc
     7081 atccgacctc ctgcaggaag ccagcagcag cctgtatcat tgaagcgtca ccaaccaggc
     7141 caatcctgcg gttcagaagg gaatgcggca caacccactc gaacctggga acgattcggc
     7201 caagttccgc attcacgaac tcggctccac gctcgcgtct tccgcacgcg gaagcgacaa
     7261 tcccgacaaa cctggaagtg ttgtcgaagc caagcggaat gggtgcctga accaccctgg
     7321 cgccggttct tgctgcaatg gcagcagcga cccgaagacc gttcggaagg gcgacgatga
     7381 ttccggaacg gcagacggcg gcaagatcgg ccacgccgca cgatgatggc cagcaggaag
     7441 acacggaaag ccccaggccg tcccggacaa gcgccgaaac ggcctcgacg tccgctttgc
     7501 cgtccggctc gttccgcgtg acgggaattc cggttattgc aaccgaaaac ggggaaattc
     7561 cggcaccggc cgaagtgatg gcgtcagcca gcgcacacat ggcgtcctcg acccccgcga
     7621 tccagtcggc accgtcccgg cgtgcaagcg gctggccgtc cctcgccagc attctttcaa
     7681 cgatatcgac ccggtgcagc ccgtcatgac gctgaaccac ccgcgccgcc tcggcgactt
     7741 cctcgggtga ggatccggtc acgttgaacc gggcgtcgcc aaccaggttc acgaccacgg
     7801 ccacgccttc caaccacggt cggccataat cctgcgggtt aaattccagc accaagcgtc
     7861 tccttgatga aagcatcgaa ttcggcgtcg gtcatcgggg tcggaaccgg gtgactttcc
     7921 ggcgcatcac ccatcatgac atcggcaagc ccctgaaata tcgcgaccac gtccgaatcg
     7981 ggatcggcca tgacaattgg aaggccggca atctcggcgc gggcaataag aggatgtctg
     8041 ggaatctcgg cgacaatcct ggttccgatg cgctgcgcga actgccgcat gccgtcgtag
     8101 ttggtctgcg gcccggtcga attgaggata agaccgccaa gcccgattcc gttggtcgcg
     8161 aaacgcttca tcgccttggc gatgttgttt gccgcgtaca gcgccatcac gccttcgctg
     8221 atgacgatat aaaccagatc cgccctgccc tgccgcaaag gcgccgcgaa tccgccgcac
     8281 acgacgtcgc caaggacgtc gaaaatcgcc gcgtcgtaat tgccctgctc gatcagttca
     8341 agctcatcaa ggacttcgaa catcctggca acgccgcgac ctccacaacc gaccccgggc
     8401 tcgggcccgc cgcactcgat acagtccagt ccaagccggc ccttgaaaac gatctcgtcg
     8461 gccgactcga tgcttccggc aaggtgatcg ataacggtgg tcaggccgct gtcgggggtc
     8521 agaaggtttc ttgtcgagtc cgccttcgga tcgcatccga cgtgaagtac cttcaatcca
     8581 cggctggcca ggatggcgct gatgttcgac gccacggtcg atttcccgat tccgcccttg
     8641 ccataaaggg caatcatttt cgtcttccgg atcaattggg tcctcttata atttcaacgt
     8701 tccagcgccg gattctacat gacttcgcgg gtgccggcac acgttttccc agcgtaatgc
     8761 caacaaattc aagtcattta ttttccgtgc cggcaagctt ccgttattct atacggaagg
     8821 acgtacgggg gccgaaccat gaatccgacc gcattccaga tattctgtgg atattacctt
     8881 ggcctggaca gcgaattcgg cgcgaagttc ttcaacgtcc attcactggc cgcccattat
     8941 ggaatcgatg ccggcacgct tcaggaattg atggcggcgc atggaatggt tcccgaaaac
     9001 atgcgccaca tcgacttcaa cgtggcaaag gcccatggaa tcgcacagga actgaccttc
     9061 accgcgtcca gggaggacgt gatcaggcac gcccgggatt ccttcgacgc atttgtccag
     9121 gcggcccgca aaatggatcc cgccaaggtt ttcgaggaca tcgattacga cgacgtgtgg
     9181 gaagacgaaa aggacaaccg cggcaaccgc tgagcccctc acacttgact gaatccctga
     9241 atcgcaggaa tcttcatcgg ggcatccggt cccgattatg accgcgagag tggtggaact
     9301 ggcagacacg ccggacttag gatccggtgc cactggcgta ggggttcaaa tcccctctct
     9361 cgcacagaca ggacgtttca tggcggtgtc cggccggtct tcggccgtgg atcagtccgg
     9421 gcaggccgac cagttcccac cggcatcacg gcactggcag gccggacggc cgctcacggc
     9481 aagccaggtc tcaatgtcgg caggctttct ggtgactgga accgaaatcc agccgcgaag
     9541 aagtgcctcg tcagcgatat cgatctcatc gcaaagtcca atccgggacg gcgcaatcct
     9601 gttcacaaca ccgacgacgc atctgcggct gtacgtttcg ggatcatcgg ttccatggat
     9661 gtcctcgatg tacagatcca cgtcatgggt cgaaatccgg tcgcccgccg aaatcgcccg
     9721 gaggtatccg ggattgacca ggatgtgggt atcccgccag aaggcatcgg ggttgtcgaa
     9781 cgcctggcct cggaacctga tatcgacggt gccgtcgtca agcgtcgtca cgaagacgtt
     9841 ccggccgaac atcgtttccc tggtgacgtc cgattccgtc gcgccaccgg tcacgagcat
     9901 cgccgtaccg aagccggtcg ggacgtattc gccgtcaccg gtgttgatcc tggcccggaa
     9961 cttcgcctgg atctccggaa tggccggatt caggtcgaaa ttgccggaca cgtcgcacga
    10021 cgcggcggga ctggttgcgg gatcaagcag gatggcggcc agctccgtgt cgaactccat
    10081 ccagggggcc ggataggtgt tgcacgtcat cgaatacgtc catcccgact cgggatagtg
    10141 gaaggtcgcg ctgcgatacc cgtagctcat tccgctgtgg ccgaacgacg tgcccagttc
    10201 atgcgtgaag ctctgggtcg cgatgccgta atcggtcggg tacccca
//
LOCUS       DAOPPA010000053        10213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7473_length_10213_cov_3.743257, whole genome shotgun sequence.
ACCESSION   DAOPPA010000053 DAOPPA010000000
VERSION     DAOPPA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7473_length_10213_cov_3.743257"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /locus_tag="PKH54_02805"
     CDS             complement(<1..2047)
                     /locus_tag="PKH54_02805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOC98846.1"
                     /translation="MTNQLPIALALVLLLAACSQTPSRPDLLMDIVLDGASDISPDAT
                     PDATSDAGQDTGPIEPGLDDPPFARHIDPFIGTQDGFFNVGSAYPGPGTPFGMVKVGP
                     DTGDEYGQVYHQHCAGYRWADPLIFGFSHNHLHGTGIPDYGDFQAMPMSAIETAKNKP
                     DSFAREYSKESEVAEAGYYAVTLRDPEIRVELTATPRCAHHRYTWPEGSTTGTIAIDY
                     GSGLVVKETSLGGHADMQDGVVLVTDHHIGDFSKVFGGFDVHAAIRFRTMPDAVGVWK
                     DDQLTRGTARIDIPKGDSVKWGGWFEFDLSAGRTVEMQVCLSYTSTTGALANMAELPG
                     WDFDGVRLAAFNAWENYVGRFRLSGGDDPRREIFYTSLYHTALMPQLASDVDGSYLGF
                     DGLVHVARDWNYYSELSLWDTFRTQQPLLALVWPEVHRDINRSLIEMARQGGNLPMWP
                     LANGDTECMIGQHAATVIADSYLKGIRDFDIDFAFDVLADAANNRLGAEVYGTRIGVE
                     PYNASGWVPADGYEASVSMTLEYAFNDFCLAELAAALELPEGEAVFRARSQNFRNVWD
                     PDLMFFRERKADGTWRAAPDKYDPTVMTFGLKNQGYTEGSGWQYRWFAPHDPAGLIAM
                     YGSADTFVTLLTEFFQTAADSFDFRTPTGFYYHGNEPDIHAAFMFNDAGRPDLAQYWA
                     "
     gene            complement(2238..4724)
                     /locus_tag="PKH54_02810"
     CDS             complement(2238..4724)
                     /locus_tag="PKH54_02810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOC98847.1"
                     /translation="MRRTGLLVLAVFVAMLGCGGEGQPDTDVVQDHGTPDAVQDPGPG
                     DAGNDTNDVVAPDDGHLDVFNDVPTDGGGDLLPTDAVDATEVVENLDEPPFARFINPF
                     IGTQDGLFNVGNAFPGPGLPFGMVRLSPDTGDKYGAGVYSHCGGYRWADPLIYGFSHN
                     HLHGTGAPDYGDFLVMPVREVTKAKTKPTTFASEYLKETETASAGYYAVTLKDPDVRV
                     ELTATTRCGHHRYTWPEGETVGAITIDYGASLVEGRSLGGTAHVAEDGSIEVLNHHLG
                     DFAARFDGYDVYSVIRFNRNPTGKGIWKDGELTRDTSEQSIAKGPEVKWGGFFEFDLT
                     GSRTVEMQVCLSYSNIESARANMAAELPEWDFEGTRQAAFDAWEGFVNRFVTKGGDRD
                     RKVNFYTALYHSAQMPQIWNDVNGNYQGFDKQVHNTDGGFNYYTDLSLWDTFRTQQPL
                     IALVWPQIHLDINKSMLKMAEQSGNLPKWALAAGDTGSMIGQHAATIVADSYLKGITE
                     FDADATFEYLKACAEDTLADGVRGRRGSATVYNTTGYIPAENSTSSVSETLEYAFCDF
                     CLAELAAALDRPEDESVFRGRSQNFRNVWDPELMFFREKNADGTWTAAVEDYDPTIMT
                     FGGKNQGYTEGSGWQYRWFAPHDPAGLIAMYGSAETFVTLLTEFFQVTKDTFNFQVPN
                     GWYYHGNEPDIHAAFMFNDAGRPDLAQYWARWILDNNYRNEPEGLVGNDDAGTLAAWY
                     VFAASGLYPSPCLPGYFVTSPAFDEVTIKLPGGDLVVKAPGASGDAIYITDVKFNGQP
                     LESTNMWITHEMVKNGGLLELTVSDTPPQG"
     gene            complement(4805..6238)
                     /locus_tag="PKH54_02815"
     CDS             complement(4805..6238)
                     /locus_tag="PKH54_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC98848.1"
                     /translation="MSDQRRVLIVDDYEPLSEFLKISLTDRGFDVTTVGTGAECMELI
                     RSGFVGTVLLDVRLPDTSGLDLLQAIKLENPILPVIMITAHASLEVAVEATRRGGFDF
                     IPKGTQITERLEVSLKNAFDHLHLKLKVLEYESSGSADKLNFSNFLTVSPRMNDLMAT
                     LRSVVNSDVAVLIEGESGTGKELIARAIHSNGSRSAKPFVAVNCAGIPETLLETEMFG
                     HEKGAFTGAASRRTGRFEAANGGILFLDEVGELPMSLQAKLLRVLQEKTFERIGGNTP
                     IQVNVRIISATNRDLMEEVKAGRFREDLFYRLSVFPVRLLPLRQRAEDIPMLAEHFLL
                     KYKKEEGKDIKGFTGPALDILKSLRFPGNVRELENLVRHAVIVATSGEITDTDIQTTL
                     RNHGIQIAPQPGGGAPQPAGYYAQFKLRDRLAAVFPTFESLVPMEALQDEYLRMAVDA
                     AGGNVTRAARRLGIGRATMYRRQKVGE"
     gene            6925..7539
                     /locus_tag="PKH54_02820"
     CDS             6925..7539
                     /locus_tag="PKH54_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOC98849.1"
                     /translation="MFDALSRAGVGLHFRVSGILCMLFLFACGPAAADSTDAGGGGAP
                     RRSGSDRVGADKAVPGPIGVDPSMGSMPVVKGRFINGVATTQAFELEVARTDAQREHG
                     LMFRQSMDPGRGMLFVFDGDAPRSFWMKNTYIPLDMIFASSDGIVVGVVENAAPLTLD
                     ERRCDVPARFVIELNAFQAARHGIKVGSLVVFDPVLERLPPVRK"
     gene            7616..>10213
                     /locus_tag="PKH54_02825"
     CDS             7616..>10213
                     /locus_tag="PKH54_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC98850.1"
                     /translation="MPDEKFAARLQEIVGRDGISTTPVDLMVYAQDMWPRATISRAVG
                     TIGSTPPDIVVWPSTVEQVIDIVRACRRAHVPFTPVAAGSGVIGGAVPRNGGVVIDVK
                     RLNRLLAVDSVSMIAVVEAGINGMHLEQMLAEKGFTLGHFPSSIMCSTAGGWAACRSA
                     GQYSSRFGKIEDMIVSMDVVLPDGRIVTLEPNQDHPGMPDFSQLVIGSEGTLGIIVRL
                     RLRLAAAPGIRRFSGFRFSKMEAGLDTMRRIMQAGVEPTMMRMYDPLDTLLNSFKAKK
                     GQGGGAGSKPESMPPLVSAALKRMGIPDFKDFGHAVTGPMLGKILQRPALVFGLMDHL
                     PLPSMLVLGFEGSEYETRRALVRANEIALAAKGTALGPEPGEHWYKKRYAVSWKLPKI
                     FAQGAFAETIEVAGLWKDVARIYHAVHSTLQSRVAIMAHFSHAYNEGCAVYFSLAGAA
                     APGQPSLDLYDWAIENAISQAMAAGGTVSHHHGIGLMKARFLDREWQGGRRVFTAIRD
                     VMDRERLSNPGKLFEAVDAPADAGEAGDSVVSVTIDKPEMVPDSPEEVRGLLVEAART
                     GRRLSRQGQGSGQGADVRVSMARIDQIIGLDPISRTVHVQAGLPMTLLESYLQEKGLT
                     LGFVPRHLMACNVGEYLATVSPSAGSPLYGTVRQNCCGLEGFLADGTPFGVGATPRRA
                     AGPDLKYCLIGAGGVYGVIVSAVLRVFPVPAVRDAVAFGTADPVAAVSSVRTLIQRGI
                     KPEWALVVSRAPADLGSRRAARLALQVGGEREDVSHSMDIIRDVMGPLGLTPEPCQPD
                     NRLQPPAEIHEYRDLFLETGPLMSLLGEMTRAEVRHGPESPEIHVTNFSTFGATIHVV
                     LREPTHKVPE"
BASE COUNT         1979 a         2942 c         3097 g         2195 t
ORIGIN      
        1 gggcccagta ctgtgcaaga tccggccgcc cggcgtcgtt gaacatgaat gcggcgtgga
       61 tgtccggctc gttgccgtgg tagtagaatc ctgtcggggt tctgaaatca aagctgtccg
      121 ccgcggtctg gaagaactcg gtcaacagcg tcacgaaggt atcggcgctg ccgtacatcg
      181 cgatcaggcc ggccgggtcg tgtggagcga accagcggta ctgccaaccg gaaccttcgg
      241 tatagccctg gttcttcagg ccgaacgtca ttaccgtggg atcgtatttg tccggtgcgg
      301 ctcgccatgt gccgtctgcc ttgcgctcac ggaagaacat cagatccgga tcccacacgt
      361 tgcggaagtt ctgcgacctc gcgcggaaca cagcttcacc ttcaggcagt tcaagcgcgg
      421 cggcaagctc cgccaggcag aagtcgttga atgcgtactc cagggtcatt gatacggacg
      481 cctcgtaccc gtccgccgga acccacccgg aagcgttgta tggctcaacg ccgatgcggg
      541 ttccatagac ttccgcccca aggcggttgt tcgctgcgtc agccagcacg tcaaatgcga
      601 agtcgatgtc gaagtcccgg attcccttaa gataactgtc tgctattaca gtcgcggcat
      661 gctggccgat catgcattcc gtatcgccgt tcgccagtgg ccacattggc aggtttccgc
      721 cctggcgggc catctcgatc agtgaacgat tgatatcccg gtgaacttcg ggccatacaa
      781 gcgccagaag tggctgctgg gtgcggaatg tgtcccagag cgaaagctcg ctgtaataat
      841 tccagtcgcg ggcgacatgc accagtccat caaagccaag atacgagccg tcaacgtcgg
      901 atgcgagttg tggcatcagc gctgtgtggt aaagggacgt gtaaaagatc tctctgcgcg
      961 ggtcgtcgcc gccggacaac ctgaagcgtc cgacatagtt ctcccaagca ttgaaggcgg
     1021 caaggcggac gccgtcgaag tcccaccccg gaagctctgc catattcgca agtgcgccgg
     1081 tcgttgatgt gtacgaaagg cagacctgca tctcgacagt tcgccctgca gacaggtcga
     1141 attcgaacca tccgccccat ttgacggaat cacctttcgg gatgtcaatc cgggcggttc
     1201 cccgggttaa ctgatcgtcc ttccagacgc cgacggcatc cggcatcgtc ctgaagcgga
     1261 ttgccgcgtg cacgtcgaac ccgccgaaaa cctttgagaa gtcgccgatg tggtgatcgg
     1321 tgaccagcac aaccccatcc tgcatgtcag cgtgtccacc cagggaagtc tccttgacca
     1381 ccaggcccga accatagtcg atcgcaatgg ttcccgttgt tgaaccctcg ggccatgtgt
     1441 agcggtgatg ggcacatctt ggcgttgccg tcagttcgac gcgaatttcc ggatcgcgca
     1501 gtgtcaccgc gtagtatccg gcctcagcta cctcagattc cttgctgtat tcgcgcgcaa
     1561 acgaatcagg cttgtttttg gctgtttcaa tcgccgacat cggcattgcc tggaagtcgc
     1621 cgtaatccgg gatgcctgtg ccgtgaaggt ggttatggct gaatccgaaa atcagcggat
     1681 cggcccaacg gtacccggcg cagtgctgat gatatacctg accgtattcg tccccggtgt
     1741 cgggtccgac ctttaccatg ccgaaaggcg ttcccgggcc gggataggcg ctgccgacgt
     1801 tgaaaaatcc gtcctgggta ccgatgaacg gatcaatgtg gcgggcaaat ggcggatcgt
     1861 cgagacccgg ttcgatcggg ccggtgtcct ggccagcgtc agaggtggca tcgggagtag
     1921 cgtccgggga gatgtccgag gccccgtcca ggacgatgtc catcagcaga tctggtctcg
     1981 acggggtctg ggagcacgca gcaagaagaa gtacaagagc aagtgcaatg ggaagttgat
     2041 tagtcatcga agtcacctga aaaccagccg tattggcgaa tgaaaattcg cccgtggcat
     2101 catgctgaac cgcggcgggc ggatgtaatc cgcccgtaca gggaaggcac cggtaatcca
     2161 tacgcttcat gggcgaacga ccttcctgca gccgtaaggg cgaatgaaaa ttcgcccgtc
     2221 gaccatcgtt atgaacatca accctggggt ggcgtgtcgg acaccgtcag ttccagcagg
     2281 ccgccgttct tcaccatttc atgggtgatc cacatgttgg tcgattcaag cggctggccg
     2341 ttgaacttca cgtcggtgat gtagattgcg tcgcccgatg cgcccggtgc cttgacgaca
     2401 agatcgccgc ccggcagctt gatcgtgact tcatcgaagg ccggcgacgt gacgaagtaa
     2461 ccgggcaggc acggcgacgg gtacagcccc gatgcggcga aaacatacca tgccgccagc
     2521 gttcccgcgt cgtcgttgcc gaccaaccct tccggttcat tcctgtagtt gttgtccagg
     2581 atccaccggg cccagtactg tgcaagatcc ggccgcccgg cgtcgttgaa catgaatgcg
     2641 gcatgaatgt ccggctcgtt gccgtggtag taccatccgt tcggaacctg gaagttgaac
     2701 gtgtccttgg taacctggaa gaattccgtc agcagggtca cgaaggtttc ggcgctgccg
     2761 tacatcgcga tcaggccggc cgggtcgtgc ggcgcgaacc agcggtactg ccaaccggaa
     2821 ccttcggtat agccctggtt ctttccgccg aacgtcatga ttgtcgggtc gtagtcttca
     2881 accgcggcgg tccatgtccc gtctgcattc ttttcgcgga agaacatcag ttcaggatcc
     2941 cacacgttgc ggaaattctg cgacctgcca cggaagacgg attcatcctc cggccgatca
     3001 agcgcggcgg caagctctgc caggcagaag tcgcagaaag cgtattcaag cgtttctgaa
     3061 accgacgaag tgctgttttc ggccggaatg tatcccgtgg tgttatagac ggtcgccgag
     3121 ccgcgtcgcc cgcgcacgcc gtcggccagt gtgtcttccg cacaagcctt caggtactcg
     3181 aaagtggcgt cggcatcgaa ttcggtgatt cccttcagat agctgtccgc gacgatggtg
     3241 gccgcgtgct gtccgatcat gcttccggta tcgccggccg caagtgccca cttcggcagg
     3301 tttccgcttt gttcggccat cttcagcatg gacttgttga tgtccaggtg gatctgcggc
     3361 cacaccaggg caatcagcgg ctgctgcgtg cggaatgtgt cccacagcga caggtcggtg
     3421 tagtagttga atccgccgtc ggtgttgtga acctgcttgt caaagccctg gtagtttccg
     3481 ttcacgtcat tccagatctg cggcatctgt gccgagtggt aaagcgcggt gtagaagttc
     3541 accttccggt cgcgatcccc gccctttgtc acaaagcggt tgacgaatcc ttcccacgcg
     3601 tcgaatgcgg cctgccgcgt tccctcgaaa tcccattcgg gaagctcggc agccatgttc
     3661 gcacgcgcgc tttcgatatt ggaataggac aggcacacct gcatttcgac ggtccggctg
     3721 ccagtcaggt cgaactcgaa gaatccgccc catttgacct cggggccctt tgcaatggac
     3781 tgctcggacg tgtcacgcgt cagttcgccg tccttccaga tccctttgcc tgtcgggttc
     3841 ctgttgaagc ggatgaccga gtacacgtcg taaccgtcga acctggcggc gaaatcgcca
     3901 aggtgatggt tcaggacctc gatgctgccg tcctcggcca cgtgcgccgt gccgccaagg
     3961 ctgcgccctt caaccaggct ggcgccgtaa tcgatggtga ttgcaccgac ggtttcgccc
     4021 tcgggccagg tgtaacgatg gtggccgcac ctggttgttg cggtcagttc cacgcgaaca
     4081 tcgggatcct tcaacgtcac cgcgtaatag ccggccgaag ccgtttccgt ttccttcaag
     4141 tattcggacg cgaacgttgt cggcttggtc tttgccttcg ttacttcacg caccggcatg
     4201 accaggaagt cgccataatc aggcgccccg gtaccatgca gatggttgtg cgaaaaaccg
     4261 tatatcagcg gatctgccca gcggtatcca ccgcagtgcg aatagacccc ggcgccgtac
     4321 ttgtcaccgg tgtcggggct gagcctgacc attccgaagg gcagccccgg accaggaaac
     4381 gcgttgccga cgttgaaaag accatcctgg gttccgatga acgggtttat gaatcttgca
     4441 aacggcggtt cgtccaggtt ttcaacgact tctgtcgcgt caaccgcgtc ggtcggcaac
     4501 aggtcgccac caccgtcggt cgggacgtca ttgaagacgt caaggtgacc gtcatccggt
     4561 gcgacaacgt cgttcgtgtc attcccggcg tcaccgggcc cgggatcctg gacggcatcg
     4621 ggcgtcccgt gatcctgcac aacgtcggta tcaggctgtc cttccccgcc gcaccccagc
     4681 atcgcaacaa aaacagcaag caccagcagt ccagtccttc tcattgacaa cccctggatt
     4741 caacgtatga agcgtgtctc aaaaagcaat cggcccggtc ggttcaaggc ccgccccggc
     4801 atcgtcattc cccgaccttc tgccgacggt acatggtagc acgtccgatt cccaggcgtc
     4861 ttgcggcccg ggttacattt ccaccggccg catcgacggc cattctaagg tattcgtcct
     4921 ggagcgcctc catcggaacg agtgattcga acgtcggaaa cacggcggcc aggcggtcgc
     4981 gcaacttgaa ctgggcatag tatcccgccg gttgcggggc gccaccgccc ggctgcgggg
     5041 cgatctgaat cccgtgattg cgcagggtcg tctgtatgtc ggtatcggtt atctccccgc
     5101 tcgtcgcgac gatgaccgca tgccgaacaa ggttttccag ttcgcgcacg tttcccggga
     5161 agcgcaggct cttcaggatg tcgagcgccg ggccggtgaa tcccttgatg tccttgcctt
     5221 cctccttctt gtacttaaga aggaagtgtt ccgccagcat cggaatgtcc tcggcgcgct
     5281 ggcggagtgg aagcagccgg accggaaaaa ctgaaagcct gtaaaaaaga tcctctcgga
     5341 accggccggc cttgacttcc tccatcaggt cgcggttcgt ggccgaaatg attcgtacat
     5401 tgacctggat tggggtgttc ccgccgatac gttcgaacgt cttttcctgc aggacgcgca
     5461 aaagcttggc ctgcagcgac attggaagct cgccgacctc atcaaggaaa aggattccgc
     5521 cattggccgc ctcgaaccgt ccggttctgc gggatgccgc acccgtgaac gcgccctttt
     5581 cgtgaccgaa catctcggtc tcaagcagtg tttccggaat cccggcgcag ttgaccgcca
     5641 cgaaaggctt ggcgcttctt gagccgttgc tgtgaatggc ccgcgcgata agttcctttc
     5701 cagtgccgga ctcgccttcg ataagaacgg cgacgtcgct gttgacgacc gaacgcagcg
     5761 ttgccatcag gtcgttcatc cgggggctta cagtcaggaa gttgctgaag ttcagcttgt
     5821 cggccgaacc gcttgattcg tactcaagaa ccttcagctt caggtgaaga tggtcaaatg
     5881 cgttcttaag gctgacttca aggcgttccg tgatctgggt gcccttcggt atgaagtcga
     5941 acccgccacg cctggtggcc tcgaccgcaa cttccaggct ggcgtgggct gtaatcatga
     6001 ttacgggaag aatcgggttt tccagcttga ttgcctgaag cagatcaagc ccgctggtgt
     6061 ccggcagcct gacgtcaaga aggacagtcc cgacgaaacc cgaacgaatc agttccatgc
     6121 actcggcgcc ggtacccaca gtggtgacgt cgaagcctcg gtctgtcagg gagatcttca
     6181 ggaattcaga cagcggctca tagtcatcga caatcagtac tcttctctgg tcggacattt
     6241 tcccggcacc gttttgtgca acgtgggcat ggtatgtcaa agtgactcaa aatgaaactg
     6301 ttgatttgaa agtgttgata aatcgggtgg ttgaaaaccg gtggaaaact ggaaatgcca
     6361 gggccgggga tgtatccggg attaccacag tcacatccac aggaagaggc ccggaccagc
     6421 aggggtttcg aaggaccccg gcacccggcg ttccagataa cgggcggcgc catctctgtt
     6481 cacacgcccg aaatttatca aagagactct tctttcaaag ggaagtgatc ttcggccggt
     6541 caaggaccgg ccgaagacga aagggggtaa agatttgaaa gaagttgggg agaaacgatg
     6601 tcaaagatga cgtgtagtgg ttattgcaat tgccgtgcca acctgaaatg tccagtaaat
     6661 tggtgacttg cggcaatccg gtgaaatttc gcggtgtctc attttgagtc agttcggcgg
     6721 cgggcattga gctgtgaaat aaaatcacaa cgtaaacagt gtatttcgct ggcgatcggc
     6781 catgtgtttc attttgaagc gaagtctcaa tttgagacat gaaaattgtt aagaggcttc
     6841 ggaatgaatg aacttggccc gaagattgcc tcactttgat ttgcgtccta tcatccatgg
     6901 ctggatgcaa cggtgacatg tcggatgttt gatgcattat cccgggccgg cgttggactc
     6961 catttccggg tttcaggcat tctctgcatg ctgtttctgt ttgcctgcgg gcccgcggcg
     7021 gccgattcga cagatgcagg gggtggcggc gcaccgcggc gatccggctc ggatcgggtc
     7081 ggcgccgaca aagccgtccc cggcccaatc ggtgtcgatc cgtcgatggg ctccatgccg
     7141 gtggtcaagg gcaggttcat taacggcgtc gcgacaacgc aggcatttga gctggaggtt
     7201 gccagaaccg atgcacagcg tgagcatggc ctgatgttcc ggcaatccat ggatccggga
     7261 cgcggcatgc tcttcgtctt tgatggtgac gcgcccagat ccttctggat gaagaatact
     7321 tacattccac ttgacatgat tttcgcctcg tcggatggaa tcgtcgtcgg agtcgtggaa
     7381 aatgcggcac ccctgacgct tgacgagcgg cggtgcgatg ttcctgccag gttcgtgatt
     7441 gaattgaacg cgtttcaggc ggccaggcac ggtatcaagg tcggttccct ggtcgttttc
     7501 gatccggttc tggaacggct tcccccggtc aggaagtgat cggaaggctt ttttcttcca
     7561 gtcctgccgc tgcctggaat atccttccgc gcaacggtga atggaggcat tctcaatgcc
     7621 cgatgaaaag tttgccgcac gcctgcagga aattgtcggg cgtgacggaa tttcgacgac
     7681 tccggtcgat ttaatggtct atgcgcagga catgtggccg cgcgcgacca tttccagggc
     7741 cgtcggcacg ataggttcaa cgccacccga tatcgttgtg tggccttcga ccgtcgagca
     7801 ggtgatcgat atcgttcgtg cgtgcaggcg cgcgcatgtt ccctttacac ccgtggcggc
     7861 cggatccggc gtgattggcg gtgccgttcc ccgtaacggc ggggtcgtga tcgacgtaaa
     7921 gcgcctgaac aggctgctgg cggtcgattc cgtatccatg attgccgttg tcgaggccgg
     7981 catcaacggc atgcacctgg aacagatgct tgccgaaaag gggtttacgc tcggtcactt
     8041 cccgtcatcc ataatgtgct cgacggcagg cggctgggcg gcatgcaggt cggctggcca
     8101 gtattcgtca cggttcggga agatcgagga catgattgtt tcgatggacg tcgttcttcc
     8161 ggacggccga atcgtcaccc tggaaccgaa ccaggatcac cccggaatgc ccgacttttc
     8221 gcagcttgtc attggttctg aaggcaccct cggaatcatc gtcaggcttc gcctgcgtct
     8281 tgctgccgcc cccggaatcc gcagattttc cggattccgt ttttcaaaga tggaagccgg
     8341 gctggacacg atgcgcagga tcatgcaggc cggtgtcgaa ccgacaatga tgcgcatgta
     8401 tgacccgctg gatacgcttc tgaacagctt caaggcgaag aaggggcagg gtggtggcgc
     8461 cgggtccaag cccgaatcga tgccgccgct ggtctcggcc gcgctgaagc ggatgggcat
     8521 cccggacttc aaggattttg gccatgccgt gaccgggccg atgcttggga agattctcca
     8581 gcggccggca ctcgttttcg ggctgatgga tcatcttccg ttaccttcga tgctggtgct
     8641 tggattcgag ggatccgagt acgaaacaag gcgggccctg gtgcgggcaa acgaaatcgc
     8701 cctggccgcg aagggaaccg cacttggtcc ggaacctggc gagcattggt acaagaagcg
     8761 ttatgccgtt tcctggaagc tgccaaagat attcgcccag ggcgcgttcg cggagaccat
     8821 cgaggttgcg ggactctgga aggatgttgc aaggatttat cacgcggttc attcgaccct
     8881 gcaaagtcgc gtggccatca tggctcactt cagccacgcg tacaacgaag gttgcgcggt
     8941 ttatttcagc cttgccggcg cggcggcccc ggggcagccg tcccttgact tgtatgactg
     9001 ggcaatcgag aacgcgataa gccaggccat ggccgccggc ggaaccgtca gtcatcatca
     9061 cgggatcggc ctgatgaagg ccaggttcct ggatcgcgag tggcagggcg ggcgaagggt
     9121 gttcaccgcg attcgggacg tgatggatcg tgaacgcctt tccaatccgg gcaagctttt
     9181 cgaggccgtc gatgccccgg ccgacgcagg cgaagcaggg gacagcgtcg tttcggtgac
     9241 gattgacaag cccgaaatgg ttccagacag tccggaagag gtgcggggac tgcttgtcga
     9301 ggcagcaagg actggccgtc ggctttccag gcagggccag ggctccggcc agggcgcgga
     9361 tgttcgcgtc agcatggcca ggatcgacca gataatcggc ctggatccga tttcgcgaac
     9421 cgttcacgtg caggccggcc tgccgatgac cctgcttgaa agctatcttc aggaaaaggg
     9481 gcttaccctt ggctttgttc ccaggcacct gatggcctgc aacgttggcg agtaccttgc
     9541 gaccgtgtcc ccttcggccg gaagtcccct ttacggtacg gtgcgccaga actgctgcgg
     9601 cctggaaggg ttccttgcgg acggcacgcc gttcggggtc ggggcgacgc caaggcgcgc
     9661 ggccgggccg gacctgaagt actgcctgat cggtgccggt ggggtttatg gcgttatcgt
     9721 ttcggcggtt cttcgtgtgt tcccggttcc tgccgttcgt gatgcggttg ctttcggaac
     9781 cgctgatccg gtggcggccg tgtcgtcggt tcgtacgctg attcagcgtg gaatcaagcc
     9841 ggaatgggca cttgtcgtca gtcgcgcccc ggccgacctt ggcagtcggc gcgccgcaag
     9901 gcttgccctt caggtcggcg gcgagcgcga ggatgtaagc cattcgatgg atatcatccg
     9961 ggatgtcatg gggccgcttg gcctgacgcc cgaaccgtgc cagcccgata accgacttca
    10021 gccgcccgcc gagattcacg agtacaggga tctcttcctg gagacagggc ccctgatgtc
    10081 gctgcttggg gaaatgacca gggcggaagt caggcacggt ccggaatcgc ccgagattca
    10141 tgtgacgaac ttttcgacct ttggcgcgac aatccacgtg gtgctgcgcg agccgaccca
    10201 caaggttccg gag
//
LOCUS       DAOPPA010000054        10153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7531_length_10153_cov_4.092097, whole genome shotgun sequence.
ACCESSION   DAOPPA010000054 DAOPPA010000000
VERSION     DAOPPA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7531_length_10153_cov_4.092097"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /locus_tag="PKH54_02830"
     CDS             <1..2012
                     /locus_tag="PKH54_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98851.1"
                     /translation="TIMPMDYESMDGEGNVVVVQSPSDGIKMLNGDTSGEFLLKKEGR
                     YILTFRLVQNQGLFRDLEIRVDGSGPMLSIIYPPRGATIDGKPSVAVVASIDDALSGI
                     TKLVVNGKEETPSKFIKNGTLTYQLPKIHQGLNTVEINIENSMGKKASETRGFFFSTK
                     YYDVDLTDLEGSMVPGAIKAYLGRSFFDDGNRNPPPDDVATLMEQIVGGLKLQNLIPN
                     PIASAGPYKVWIRKIEYDKPSVSLSPETDYLALGIDIRNFHLEVEAEGKCQFIIDWCP
                     DVTGHVYADAFEVDTELHIKMDENAVFTSTMKFANFNIAGLDIDLEGFIGDLLDWLID
                     SIVDVFADAIEKAIKGQLETMLNDVIVDLLDQFKIETSLEIPSILGSKPVEVSLMTMP
                     SKFKLDHPGMWLDMDAVIYSQKGVAHENLGSIGRANCLKVNTDPFVIPEDAQVGFAAY
                     DDVLNQILYAVWNAGTLNLTIGEDILGGLDIPVVVLKKVDLDFYSPPVITGCNPEYVF
                     DPETFEGDCTVAPEQVMQLQLGDAYARIEGDVFGMPMYMEVFVQAIGLVDLAAGEDEA
                     GNSTIKIRLLNVPKIMLDVISVDPEFLATNGWTKDQFYNDIILPMIGPLLEQLPTCEQ
                     LLDFPIPVIDLNSLVSDIPTGTNVKFFVEQFFRQNGFTAVQGHLE"
     gene            complement(2063..4777)
                     /locus_tag="PKH54_02835"
     CDS             complement(2063..4777)
                     /locus_tag="PKH54_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98852.1"
                     /translation="MKSSAARHFSFIVLLVAAGCSGNVVYTDTSGDDVGHDAVVQDTG
                     TEDDAGDVVVFPDMGGNGTVSATLPSAEMAVRITTPSNRGYYATQAGTVALGGIYFGN
                     LESIVFEAEGVTGGQVISDPSSPYWQTAAISLKPGDNLITVIASGRGDRDDVEQPPVT
                     VRDSIVITYNPNMYFPAPMRMSPPTVFSGRTQAVLAKVNMGLFGTAAGDRMYVVRQFE
                     DGRLSENLGGMSDGGMVNGSADEIQGDGVFTGRVTVKCDAVGTQLYRAALEVTSDSGS
                     YVALSAPFELRCIENIDSATCSAHKETLSKATKAFMAKVDAGMPEQARAAAFKVIAAD
                     TSIAETSQSDESGGGIWVRFNDGVLGALNLYAEGLRGGGGSGVDEVPVSLRAADLRSL
                     DRNQISSRETLLLAPFFTEFGRQDEIPAIAEIVSEINCPVFTLKGPYLGSGASLEKFR
                     QLSTSGIIAISTHSDVYFAGLSGAVRAGLPWRHRGGQEILWSGESVNCGRIGSSDQEC
                     ESSADCPAGSTCIITDPPEEYTEQVETDAGIQQVTKMTKPSGTCYDTTHADLMSGRLV
                     MGDRNWGVTPEFVEYYADEQRLPGSLVYLGGCRTLYNGSLAMALLASGARTVLGYTNR
                     VSSEFAFKSGRDFFFNFLLRTLSASESYGGGDVDPDNPGGFFRLFGALDLSVKYSNIL
                     NPGFETSDAAAWVTQGDGRVITRLGETEPIAGKFMGIISTGLGFTDKTGTISQSFCIP
                     AGARELVFYWKYYSEEFHEFCGTLYQDAFVARLTTKDGTEYPVVDIKVDDLCAKDGST
                     TPPELCFEYEGSCDPEKCTNKVSGKLECRKYHCEADQCGSKFVGLEPSDIDFDRGDTY
                     MTGWQKAVFPLDAFDPERDAPVTISFYCTDKGDSIFDTAVLVDSVSFR"
     gene            5000..6286
                     /locus_tag="PKH54_02840"
     CDS             5000..6286
                     /locus_tag="PKH54_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOC98853.1"
                     /translation="MADAGLVIIGAGLSGLTTALFSRDDFLLLEKSSRPGGLLKTEDI
                     EGFLFDFTGHWLHLRDPRTKDFIADLMGDGMVTVNRKAAIWSNGVMTLFPFQANLFGL
                     PPAVIRECLQEAVASAVSRAAGTAAVPGNFEEYANIHFGRGIARHFIVPYNTKLWGVP
                     PSEVTAEWTQRFVPMPDIAQIIDGALGFSAQAMGYNVSFIYPATGGIESIPRAMAARL
                     DHSRIMFNTAPTALNAEERWVEAGGRRISWDRLVSTTALPDLVKMTVDAPDVIRKAAA
                     ELRCSPLRFVNVALDVPTPLDGNHWIYLPETKYPFYRVGCFSNAVKSLAPAGKSSLYV
                     ELAPDTTMPEAEITAALQDFLIEIGSITEKSQLLFARYRNHPWGYVIFDERCVPARDL
                     ILGWYRSKGIKSIGRYGACTYNSMEDAMLEAMAAAG"
     gene            6320..7039
                     /locus_tag="PKH54_02845"
     CDS             6320..7039
                     /locus_tag="PKH54_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC98854.1"
                     /translation="MGSERTEVSIVIPVFNEEGLLETAINDLVLKLNELGWEKEIIIT
                     ENGSTDRTVEIANDLARRYPFLKVLHTGEPNYGKALRKGIETASGVFVMCDEIDICDV
                     NFHKAALEILKGGEFDMVVGSKRHPDARDARPAFRRFATWVVNMMLRVATGFRGTDTH
                     GLKAFVREATLPVVSRCIVEKDMFASELVIRAERLIRVKEIPVTIVEKRPPAINLFKR
                     VPNVLRNIFRLFLIIRLGKKA"
     gene            7057..7959
                     /locus_tag="PKH54_02850"
     CDS             7057..7959
                     /locus_tag="PKH54_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC98855.1"
                     /translation="MNRVLAVNIDLDDLRFYRGIHALPQQPENAEIFKLGLPRFLDLC
                     ERHEIKATLFVITDDLKIPGVSEAIRDAARRGHEIASHTRSHDYGISRRDPDSIAAEL
                     AGSRRDILNACGIEPTGFRGPGYNLTPDLLDGLAAAGFLYDTSIMSSPPYWLARALII
                     AAMAATGRKSSSITGRARDFFRGHRPFVWGGPGKGLVEFPITTAGPLFLPLIGTTLAG
                     RGALVRQAVTIARGREFVNIEFHGIDFLDREADGLEPELLVEPALKIPLAERMHNFDL
                     ALRMLGEGRRSPTLATLAASFNAS"
     gene            8022..8456
                     /locus_tag="PKH54_02855"
     CDS             8022..8456
                     /locus_tag="PKH54_02855"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOC98856.1"
                     /translation="MNDCIFCKIVQGLIPSAKIYEDGDFLAFLDIRPANHGHVLIIPK
                     VHAERVTDLPESILSRELVLAARIAPAVLAATGMTDFNLFNTNGAASGQEVFHHHLHV
                     IPRKAGDGMKLSIDPQTYREGEMAEVAGAIRRRLDLREPGES"
     gene            8456..10144
                     /locus_tag="PKH54_02860"
     CDS             8456..10144
                     /locus_tag="PKH54_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98857.1"
                     /translation="MRGMDSFSIISLMKPAFIPLPREAGGSHSKIYFIDEQKLGAILA
                     EAAVPGAREKARELGIRIGSRFIVKVGNIHDYIVKLKDGRESVIRRQHPSEAFGPGGY
                     NLAPTVGKVLTDVREAARQIPFMSFGSRRQQLAPDVLGDIARCISGRPEGDSADGTFL
                     VQDTPDGIRKMSIRQLLTEDPFFRSRIGSFEELRRYQEFLRVNPIDSATETILGDFFN
                     SPITVGFRDTLRFLEGSRHPSLGWDRARAVILESFEKYGRETFLPPEFNYYTSSMEYS
                     PECVLDMNRENSRAAASFAELLKERAPADTFAEMGEKEIEAVASAILEIHFDYPEFEV
                     DPVSGVFSTATARLVARSAFWATIQAADVVLPDRRNAITDEIARRSSALAFFLDRMDE
                     VEREDFLSLVNRILWEVAIHGGVPDNQGRIWPWKRVNRTFFSFQELAQYQMKSRGTLE
                     ETVTLEDLYRPSGRKILQAHPELPGKLLVFFVLAYRYYTETGHIPDLRPDDAGIDLFV
                     RGTYGYSTRNVLVALGRDRDGRPDSDVRFIDNRDQFKQYRRWEDRDRVSALKQG"
BASE COUNT         2141 a         3041 c         2978 g         1993 t
ORIGIN      
        1 taaccatcat gccgatggac tacgaatcga tggacgggga aggcaacgtc gtcgtcgtcc
       61 agtcgccttc ggacggaatc aagatgctga acggcgacac gtccggcgaa ttcctgctta
      121 agaaggaagg gcgatacatc cttacgttca ggctggtcca gaaccagggg ctgttcaggg
      181 atcttgaaat ccgcgtcgat ggcagcgggc cgatgctctc gataatttat ccgccacgcg
      241 gcgccacaat cgacggcaag ccttcggtcg cggtcgtggc gagcatagac gatgccctgt
      301 ccgggatcac gaagctggtg gtcaacggca aggaggaaac tccttcgaaa ttcatcaaga
      361 acggaaccct gacataccag ttgcccaaga tccatcaggg cctgaacacc gttgaaatca
      421 acattgaaaa cagcatggga aaaaaggcct ccgaaaccag gggcttcttc ttctcgacca
      481 agtattacga cgtcgatctg accgaccttg aaggctcgat ggttcctggc gcgatcaagg
      541 cataccttgg caggagcttc ttcgacgacg gcaaccggaa cccgccgccg gatgacgtgg
      601 cgaccctgat ggagcagatt gtcgggggcc tgaaactgca gaacctgatt cccaacccga
      661 tcgcgtcggc cggaccctac aaggtctgga tacgcaagat cgagtacgac aaaccgtcgg
      721 tcagcctgag ccccgagacg gactacctcg cgcttggcat cgacatcaga aatttccacc
      781 tggaggtcga ggccgaaggc aagtgccagt tcatcatcga ctggtgtccg gatgtcaccg
      841 ggcacgtata tgccgatgca tttgaggtcg ataccgaact gcacatcaag atggacgaaa
      901 acgccgtctt cacatcgacg atgaagttcg ccaatttcaa tatcgcggga ttggacatag
      961 atctggaagg ctttatcggc gacctgcttg actggctgat cgattcaatc gtcgatgtct
     1021 tcgcggacgc catcgagaag gccataaaag gccagcttga aaccatgctg aacgatgtca
     1081 tcgtcgatct cctggatcag ttcaagatcg agacttcact ggagattcct tccatcctcg
     1141 gttcgaagcc ggtggaagtt tccctgatga cgatgccttc aaagttcaag ctggatcacc
     1201 ccgggatgtg gctggacatg gacgcggtca tctattcgca gaagggcgtc gcccacgaga
     1261 acctgggatc aatcggacgc gccaactgcc tgaaagtcaa caccgacccg ttcgtgattc
     1321 cggaagacgc ccaggtcggc ttcgcggcgt acgacgacgt cctgaaccag attctgtatg
     1381 cggtctggaa cgccggaacg ctgaacctga cgattggcga ggacatcctt ggcggacttg
     1441 acataccggt cgtcgtgctg aagaaggttg acctggactt ctattcgcct ccggtcatca
     1501 ccggatgcaa tccagagtac gtctttgacc cggagacgtt cgagggggac tgcacggtcg
     1561 cgccggaaca ggtcatgcag ctccagctcg gcgatgcgta tgcaaggatt gaaggcgatg
     1621 tgttcgggat gccgatgtac atggaagtgt tcgttcaggc catcgggctt gtcgatcttg
     1681 cggcgggcga ggacgaggca ggcaactcga caatcaagat ccgacttctg aacgttccga
     1741 agatcatgct cgacgtcatc agcgtcgatc cggaattcct ggccaccaac ggatggacca
     1801 aggatcagtt ctacaacgac ataatccttc cgatgatcgg gcctctgctc gaacagcttc
     1861 caacctgtga acaactgctg gacttcccga tcccggtcat cgacctgaac agcctggtgt
     1921 ccgatatccc gacgggaacc aacgtcaagt tcttcgtcga gcagttcttc aggcagaacg
     1981 gcttcaccgc ggttcaaggc caccttgaat agaatcgcgc ccgcgccgac gccatgattc
     2041 cccggaagtg atttcctgtt tgttacctga agctgaccga atcgaccagc acagcggtat
     2101 cgaagatcga gtcgcccttg tcggtgcagt aaaacgatat cgtgaccggc gcgtcgcgct
     2161 cgggatcaaa ggcgtcaagc gggaagacgg ccttctgcca tccggtcatg tacgtgtcgc
     2221 ccctgtcgaa atcgatgtct gatggctcga ggcccacgaa cttgctgccg cactggtcgg
     2281 cctcgcagtg atactttcgg cactcgagct tgccgctgac cttgttcgtg cacttttcgg
     2341 gatcgcacga gccttcgtat tcaaaacaga gttccggcgg cgttgtcgaa ccgtctttcg
     2401 cgcacaggtc atcgaccttg atatccacga ccggatactc ggtgccgtcc ttcgtcgtca
     2461 ggcgggcgac gaatgcgtcc tggtaaagag tcccgcagaa ctcgtgaaat tcctcggagt
     2521 agtacttcca gtaaaaaacc agttcccggg cgcccgccgg gatgcagaaa ctctgcgaaa
     2581 tcgttccggt tttatcggtg aatccaaggc cggtcgagat gattcccatg aacttgccgg
     2641 cgatcggctc ggtttcgccg agcctggtga ttacgcggcc gtcaccctgg gtgacccagg
     2701 ctgccgcgtc cgatgtttcg aacccgggat tcaggatgtt cgagtacttg actgacaggt
     2761 caagggcacc aaagagcctg aaaaagccgc cggggttgtc cggatcgacg tcgccgccgc
     2821 cgtatgactc gcttgccgac aatgtgcgaa gaaggaagtt aaagaagaag tcgcggcccg
     2881 acttgaatgc gaattcggaa gagacccgat tggtgtagcc aaggaccgtc ctggcgccgg
     2941 aggccagcag cgccatggca aggctgccgt tgtaaagcgt cctgcagccg cccagataca
     3001 caagacttcc cggcaggcgc tgctcgtcgg cgtagtactc gacaaactcc ggcgtgacgc
     3061 cccagtttcg atcgcccatg accagacgtc ccgacatcag gtcggcgtgg gtcgtgtcgt
     3121 aacacgttcc cgagggcttc gtcatcttgg ttacctgctg gatcccggcg tcggtttcga
     3181 cctgctcggt gtattcctcc ggcggatcag tgatgatgca ggtggagccc gccgggcagt
     3241 ccgccgacga ctcgcattcc tggtcggacg acccgatcct gccgcagttg accgattctc
     3301 cgctccacag gatctcctgg ccgccacgat ggcgccacgg aaggccggcc ctgacggcgc
     3361 cggaaaggcc ggcgaaatag acgtcggagt gcgtcgatat ggcgatgatt ccgctggtcg
     3421 aaagctgcct gaacttttcc aggctcgcgc ccgacccaag atacgggccc ttaagcgtga
     3481 agaccggaca attgatttcg gaaacgatct cggcaatcgc cggaatttcg tcctgacggc
     3541 cgaattcggt gaaaaaaggc gccagcagaa gcgtttcgcg gctgcttatc tggttgcggt
     3601 cgagcgaccg caaatcggcc gcgcgcagac tgaccggaac ctcgtcgacg cccgatccgc
     3661 caccgccacg caggccttcg gcatacaggt taagcgcccc gagcacgccg tcgttgaacc
     3721 tgacccagat accgccgccc gattcatcgg actggcttgt ctcggcaatc gacgtatcgg
     3781 ccgcaatcac cttgaaagcg gcagccctgg cctgttccgg cattccggca tcgaccttgg
     3841 ccatgaacgc ctttgtcgcc ttgctcagag tttccttgtg ggccgaacaa gtcgcggaat
     3901 caatgttctc gatgcatctg agctcaaatg gcgcggacag ggccacataa cttcccgaat
     3961 ccgacgtcac ctcaagcgcc gctcgataga gctgggttcc aacggcatcg cacttgaccg
     4021 ttacccgccc ggtgaaaacg ccgtcgccct gaatttcatc ggccgatccg ttaaccatgc
     4081 cgccgtccga catgcccccc aggttttcgg aaagacgacc gtcctcaaac tgccttacga
     4141 cgtacatgcg gtcgcccgcc gccgtgccga agagtcccat gttgaccttt gccaggacgg
     4201 cctgggtcct gccggaaaag acggttggcg gcgacatgcg catcggtgcc gggaagtaca
     4261 tgttcgggtt gtaggtgatt acgatgctgt ccctgaccgt gaccggcggc tgctcaacgt
     4321 cgtccctgtc cccgcgcccg ctggcgatta ccgttatcag attgtcgcca ggcttcaggg
     4381 aaatggcggc agtctgccag taaggacttg acgggtcact gatgacctgg ccgccggtga
     4441 cgccttcggc ctcgaaaacg attgattcca ggtttccgaa gtatatgccc ccaagggcaa
     4501 ccgtgccggc ctgagtcgcg tagtacccgc gattcgacgg tgtcgttatc ctgaccgcca
     4561 tctcggccga tggcagggtg gccgagacgg ttccgttgcc gcccatgtcg ggaaacacga
     4621 cgacgtcacc tgcatcgtct tcggtaccgg tgtcctgcac gacagcatcg tgaccgacgt
     4681 catcgccgga cgtatcggta tagacgacat tgccggagca cccggccgcg acgagcagaa
     4741 cgataaatga aaaatgcctt gcggcggacg acttcatcaa aacctccgcg gatcgccatc
     4801 gaattgccgg gccctggcgc acgcggggtc cgctgcgccg attgtaagga aggaccgtgc
     4861 cgccttcaat gatactaccg attaatagta agggcggcat tgttgaaggg gggaaccttt
     4921 tcatgacgtc accgtcggcc attgaccttg aagccgcaat cctgtagttt ttgcgcggca
     4981 atcccaattc gggggcacga tggcggacgc cggactcgta atcatcggcg caggcctttc
     5041 cggcctgacg acagcgctct tttcaaggga cgatttcctt ctgctggaaa aatcgtctcg
     5101 accgggcgga ctgctgaaga ccgaggacat cgaaggattc ctgttcgatt tcaccggcca
     5161 ctggctgcac cttcgggacc cccggacaaa ggatttcatt gccgacctga tgggggacgg
     5221 aatggtcacc gtcaacagga aggccgccat ctggtcaaac ggcgtcatga cgctttttcc
     5281 cttccaggcc aacctgttcg gactgccgcc ggccgtcatc cgggaatgcc tgcaggaagc
     5341 cgttgcctcg gccgtcagcc gggcggcggg aacggccgcc gttcccggca atttcgagga
     5401 atacgcgaac attcatttcg gacgtgggat cgcgcggcat ttcatcgttc catacaacac
     5461 gaaactgtgg ggcgtcccgc cttccgaggt gaccgcggag tggacccaga gattcgtgcc
     5521 catgccggac atcgcccaga ttatcgacgg cgccctcgga ttttcggccc aggcaatggg
     5581 gtacaacgtt tcgttcattt atccggcaac cggtggcatc gaatcgatcc cccgcgccat
     5641 ggcggccagg cttgaccatt caaggattat gttcaacacg gctccgaccg cgctgaacgc
     5701 cgaagaacgc tgggtcgaag ccgggggcag acggatttcc tgggacaggc ttgtaagcac
     5761 cacggcgctt cccgacctgg tgaagatgac cgtcgatgcc cccgacgtga tcaggaaggc
     5821 cgcggccgaa cttcgctgct cgccgctgag gtttgtaaac gtcgcgctgg acgtgccgac
     5881 cccgctggac ggcaaccact ggatttacct gcctgaaacg aaatatccgt tctatcgcgt
     5941 cggctgtttc tccaacgccg tgaaaagcct tgccccggcc ggcaaatcgt ctctgtacgt
     6001 cgaactggcc cccgacacca cgatgcccga ggccgaaatc accgcggcgc ttcaggactt
     6061 ccttattgaa atcggcagta tcaccgaaaa gtcccagttg cttttcgcca ggtaccgcaa
     6121 tcatccgtgg ggatacgtaa tcttcgacga acgctgcgtg ccggcccgcg acctgattct
     6181 gggctggtac cggtcaaaag gtataaaatc catcggccga tacggggcct gcacttacaa
     6241 ttcgatggaa gacgccatgc tggaagcgat ggccgctgcc ggctgaacag ggcgcccgaa
     6301 cgcccggggg tgcatcaata tgggatcgga aagaaccgag gtctcaatcg taataccggt
     6361 gttcaacgaa gagggactgc tggaaacggc gatcaacgac cttgtgctca agctgaacga
     6421 gctcggctgg gaaaaggaaa tcatcatcac cgaaaatgga agtaccgatc gcacggttga
     6481 aatcgcgaac gaccttgccc ggcgctatcc gttcctgaag gttcttcaca ccggcgaacc
     6541 gaattatgga aaggccctgc gcaagggcat cgaaaccgct tccggcgtct tcgtcatgtg
     6601 cgatgaaatc gacatctgcg acgtcaactt ccacaaggcg gcacttgaaa tcctgaaggg
     6661 cggcgaattc gacatggttg tcggaagcaa gcggcacccc gacgcgaggg acgccaggcc
     6721 ggccttccgc cgtttcgcga cgtgggtcgt caacatgatg ctgcgcgtgg cgaccggatt
     6781 caggggaacc gacacgcacg gactgaaggc gttcgtccgc gaggccacgc tcccggtcgt
     6841 ttccagatgc atcgtcgaga aggacatgtt cgcgagcgaa ctggtcataa gggccgaaag
     6901 acttatccgg gtgaaggaaa tcccggtgac catcgtggaa aagcgcccgc ccgcgatcaa
     6961 ccttttcaag cgcgtcccga acgtccttcg caacatcttc aggctgtttc tgatcatccg
     7021 cctgggaaag aaggcctgat gattccgggg gccttgatga accgggtact cgcagtaaac
     7081 atcgatcttg acgatcttcg cttctatcgc gggattcacg cccttccgca gcagccggag
     7141 aacgcggaaa tcttcaagct gggactgccg cgcttcctgg atctgtgcga aaggcatgaa
     7201 atcaaggcaa cgctgtttgt aatcaccgac gacctgaaaa ttccgggtgt gtccgaggcc
     7261 atccgggacg cggcccgccg tggccacgag attgccagcc ataccagatc ccacgattac
     7321 ggcatctccc gccgcgatcc ggattcaatc gcggccgaac tggccgggtc gcgcagggac
     7381 atcctgaatg cctgcggaat cgagccgacc ggctttcggg ggccgggcta caacttgacg
     7441 ccagacctgc tggacggcct tgcagcggcg ggctttcttt acgacacgtc aatcatgtcc
     7501 agtccaccgt actggctggc gcgcgccttg attatcgcgg caatggcggc cacgggcagg
     7561 aagagttcct cgattacggg aagggccagg gacttcttca ggggacatcg cccgttcgtc
     7621 tggggcgggc cggggaaggg cctggtcgaa tttcccatca caacggcggg ccccttgttc
     7681 ctgcccctga tcggaacgac gctggctggc cggggcgcgc ttgtccggca ggcggtcacg
     7741 attgcccgcg ggcgcgaatt cgtgaacatc gaattccacg gaatcgactt cctcgaccgc
     7801 gaggccgatg gacttgagcc tgaactgctg gtcgaaccgg ccctgaagat cccgcttgcc
     7861 gagcgcatgc ataatttcga cctggccctg cggatgcttg gcgaggggcg ccgaagcccg
     7921 accctggcaa cgctggccgc ttccttcaac gcatcctgac aagtcttcaa tcatttcctt
     7981 tttcatgtga caatccgatt cccgtgcttt gaagaggcaa catgaacgac tgcatcttct
     8041 gcaaaattgt tcagggcttg attccatccg cgaagattta tgaagacggt gatttcctgg
     8101 cattcctgga catcaggccg gcgaaccacg gccacgtgtt gatcatcccg aaggttcacg
     8161 ccgaacgcgt cacggatctt ccggagtcca tcctttcaag ggaactggtg cttgccgcaa
     8221 ggatcgcccc ggcggtgctg gcggcgaccg ggatgaccga cttcaacctg ttcaacacca
     8281 acggcgcggc ttcagggcag gaggttttcc accaccacct gcacgtcatt ccaagaaagg
     8341 ccggtgacgg aatgaagctg tccatcgacc cgcagaccta ccgggaaggg gaaatggcgg
     8401 aagttgccgg cgctatccgg cggcggcttg accttcgtga accgggggaa tcgtgatgag
     8461 gggcatggat tccttttcga tcataagcct gatgaagccg gccttcatac cgctgccgcg
     8521 cgaggccggc ggttcgcact ccaagattta cttcatcgac gagcagaaac tgggcgccat
     8581 cctggccgaa gcagccgttc ctggcgccag ggaaaaggcg cgtgagcttg gaatcaggat
     8641 tggaagccgg ttcatcgtca aggtcgggaa catccacgac tacatcgtca agctgaaaga
     8701 tggtcgggag tccgtgataa ggcgtcagca tccctcggaa gcattcggcc ccggcggata
     8761 caacctggcg ccgacggtcg gcaaggtgct taccgatgtt cgcgaggcgg cgcggcagat
     8821 accgttcatg tcgttcggtt caagacgtca gcagcttgcg cccgacgtgc tgggcgacat
     8881 cgcaaggtgc atttccggca ggcctgaagg cgactcggcc gacggcacct tccttgtcca
     8941 ggatacgccg gacggaatca ggaagatgtc catccgtcag ttgctgaccg aggatccttt
     9001 cttcagaagc agaatcggat ctttcgagga actgcgacgc taccaggaat tcctgcgcgt
     9061 caacccgatc gattcggcaa ccgaaacgat cctcggggac tttttcaatt cgcccataac
     9121 ggtcgggttc cgggacacgc tgcgctttct cgaaggctcc aggcacccgt ccctgggatg
     9181 ggacagggca agggccgtaa tccttgaaag ttttgagaaa tacggaaggg aaacgttcct
     9241 cccgccggaa ttcaactact acaccagttc gatggaatac agcccggaat gcgttctgga
     9301 catgaatcgg gaaaactccc gcgcggcggc cagcttcgct gagctcctga aggaacgggc
     9361 gccggccgac actttcgcgg aaatgggcga aaaggaaatc gaggccgttg cttcggccat
     9421 tctggaaatc cacttcgact acccggaatt cgaagtcgat ccggtcagcg gcgtgttcag
     9481 caccgccacg gccaggctcg tcgcgcgatc cgcgttctgg gcgacaatcc aggccgccga
     9541 cgtcgtgctg ccggacaggc gaaacgccat caccgatgaa atcgcaaggc gttcaagcgc
     9601 cctggcattc ttcctggaca ggatggacga ggtcgaacgc gaggatttcc tttcgctggt
     9661 caaccggatc ctgtgggaag tcgccattca tggtggcgtg ccggacaacc agggacggat
     9721 ctggccctgg aagcgcgtca accgcacgtt cttcagcttc caggagctgg cgcagtacca
     9781 gatgaaatct cgtggcaccc ttgaagaaac cgtcacgctg gaggatcttt accgtccatc
     9841 gggccggaag attctgcagg cacaccccga actcccgggc aagctcctgg tctttttcgt
     9901 ccttgcgtat cggtactaca cggagaccgg ccacattccg gatcttcgcc ccgacgatgc
     9961 cggcatcgat cttttcgttc gcggaaccta cggttattcg accagaaacg tcctggtggc
    10021 cctgggccgc gaccgggacg gcaggcccga cagcgacgtc agattcatcg acaaccgcga
    10081 ccagttcaag cagtaccggc gctgggagga cagggacagg gtatccgctc tcaagcaggg
    10141 atgaaaccgg tct
//
LOCUS       DAOPPA010000055         9944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7727_length_9944_cov_3.829002, whole genome shotgun sequence.
ACCESSION   DAOPPA010000055 DAOPPA010000000
VERSION     DAOPPA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7727_length_9944_cov_3.829002"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="PKH54_02865"
     CDS             complement(<1..2261)
                     /locus_tag="PKH54_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC98858.1"
                     /translation="MSGEPPILVIEKEGGHAIRDAVGIDGVKVESADVAGGSARGLDA
                     LRDRTSDRIFAAVIIDPSALVPDVAAPGTDPAAVKTLANAIRSAASRNLSTSFILVGL
                     EGVDAAAVRDAVIKGAATEDDAPVDLERALTAVSIGSSAELKRLVTEAASCFGSTAQF
                     KSGGRTSFLPPLEPMITSLWFDVFGYNEFSARLTGVLFAVLTLALLVFGTRRLFSDAI
                     PAGALFVLATSLLFYGTARFAGAGMAEVFFVALTALSFGQIVMPCEDEKCPVRKWGPW
                     VLMTAGLVLCWLSGGMTAVVTAAAIAGTWFAVSPDRRHLPAVVAVFALLGVLAIATFI
                     PEAAWFRQFRFTAATFTGGMSLESRTFDFIVKEIGFGFFPWSAFIPVAIWMAAAGSDT
                     GRPARMLLLLWAVVPMVTAMVFIRPFGQTSYLGLPGMAMLTALFFRDFEDRDRDGVLA
                     ARIFGFFAAGLFLVIMKDMLKSPAPLVTFLTTDPMFSKPGQGDAGFPSTVQIPTIGKL
                     LLALMLISILSVASRVSNIAVLVKSFMAGRKAFIAAVATAGGLILIDIIVFIALKWPA
                     LTKPGAGSEWLRIFLTGPDILALYLLLGIVFAIHWWDLIWARASSVIGSKWSSRLESA
                     FFDFQTLKVQRPLFIACIAGFTAVQIFSVMPELSRHLSQKHIVDTWKDAARKEAGPLF
                     RHGTFSSRGSEDSNFYTANIEEKASRSEVTSMLADKSRRTFFLVPTRQFSEINSAWRD
                     QTRERGLPVLDDRS"
     gene            2521..4200
                     /locus_tag="PKH54_02870"
     CDS             2521..4200
                     /locus_tag="PKH54_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HOC98859.1"
                     /translation="MITRSASARDVPVLAGRSAALALPPDFPSLSGTARLDAILKLAS
                     PGKFISRLREDEFVFLVNGIGLEDAGVLVRYATSAQRKLLVDMDSWEGDEFRPDRFDR
                     ILDIVKEHGLDFTVEMLRELDPGILILSLVKRAKFHTVEEAEDMEFAPGTWFMTPDGT
                     FVVECADEDDVPAVRNLIDLVYAIGVAYAHWLIQGGRWETVSSVQEQSFEYRRSRLAD
                     NGFPEEEDPYSIYEPFDLESFRSRVAGTDPGETSAGTARGEPLALTVADTPKGLFFWK
                     VISSPAAAGLDHAAILSQTMNLVNRVMGASVRDLSEVESWGPVAAHALTVVSVGLEAV
                     SRGDVEVAASVLKKSVPLELFRAGIEFSRPVSVLARQVVADVGGLASLSLFGDSAAAD
                     IDAAAAFPAKCPPSISGSDGEYVDFQNCGQVIASISMMKKFKAVVQFSKAVLGFDPSG
                     NHSGDGLLHPPRFASVVATAWARQVLDGALSLAPLRGADLVRLRGIAFDGSVIRPGLR
                     LSASGEGDEYALAVREFISESLDSVEAGLGGLVDADMVDPRLIGDCLLIAE"
     gene            complement(4210..5826)
                     /locus_tag="PKH54_02875"
     CDS             complement(4210..5826)
                     /locus_tag="PKH54_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98860.1"
                     /translation="MRNLLKAVVLTAVLAIGLTTFTGCARGTPTTCKGWVKLLKSPVN
                     GKKAIQNLGELKCAESIPALAEIFPRSQYKDDILLAVQQINAPEASLELVNAALADPE
                     AAVQAAAVAEDFARPEFRAGLKEILESNKALDARMNALKALAKIDKDNLIQHEDLCIK
                     LLLEDPNLQKIEVNAEAAKLLGQMKSEKAIGALIKAVFIRDQRGRQAYVPVRRALAEI
                     GRPAVEPLVGVLTKDVQNYGRLIEEINAMAGKMGIFDWQIFDGPELVQILGDLRDERA
                     ALALAKDLGSVLNPPVGVDDRVIRTWQIAQQNRITMSMMALWNVGNKEIIPTLVETIT
                     NEDNDAKQRLDTASSLALVPGFAGIPPLLKIFKETKREVFRAPLVKVISLGIDWQNYA
                     VFKKMLAAEKSDLVKERFQGDNAEAQAFLATVKPLEQCKPFDVECLVQMTKDENTTVG
                     QKAALLLGGVTEPEARAAALKALIELYPQIDPKRDVDFRRFVLLSIWRLGDKSIMPEM
                     ERLLAADRANKRANYWVEELQTFLPAMATK"
     gene            5920..6474
                     /locus_tag="PKH54_02880"
     CDS             5920..6474
                     /locus_tag="PKH54_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC98861.1"
                     /translation="MRLNSLPALMLLLATSCGGWTDGGQPVGIGGPTAGSPEYRALLG
                     GDICRADRDCVTEICSDGTCIGFLMISSDAGRNDVGALIRERFGESPASLSRLASIAS
                     GMIEDPLTEVYFRGRSADFFRFLPCAMSQPMLRDKLASRDEPVRFFAARVLSLCGDQN
                     GREILDGFLSHPSEAIRRMAAAVQ"
     gene            complement(6468..7106)
                     /locus_tag="PKH54_02885"
     CDS             complement(6468..7106)
                     /locus_tag="PKH54_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOC98862.1"
                     /translation="MIWAGKRAAIVETCRRMYARGLVSGGQGNVSVRLTDELILITPS
                     GVNKGFIRPEDLVVASMDGRPVDPDLEVSSEIKVHLACYRNRPDCGAVVHAHPVASVA
                     LTLAGLSLEPAFLPESVITLGSVPTGRYATPSSVELAQDVGRLAVDHNVIMMERHGAV
                     CLGSDPFMAYDRLESLEHNSRIVLLARTLSQPSPLPDAEVERLRIIAAGMDY"
     gene            complement(7103..7501)
                     /locus_tag="PKH54_02890"
     CDS             complement(7103..7501)
                     /locus_tag="PKH54_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98863.1"
                     /translation="MNRPATLVLMLAAGLATACSNSPSKVLNELEEAAVRGDPAAFGS
                     HFSTQSRPFAEALLSIQKEAWSGEKTPIEKFSRSRIASEKISGDTALVLTIAPDGTEV
                     PVFFVREAGAWKLDAASTASGRPQSKAGDE"
     gene            complement(7563..7648)
                     /locus_tag="PKH54_02895"
     tRNA            complement(7563..7648)
                     /locus_tag="PKH54_02895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7613..7615),aa:Leu,seq:gag)
     gene            complement(7694..8092)
                     /locus_tag="PKH54_02900"
     CDS             complement(7694..8092)
                     /locus_tag="PKH54_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98864.1"
                     /translation="MKKSIVVVVTAFISLAIAGCGRNPCRNLATEVCERAPKTVACEA
                     AGKLTADDECNDYLKNLDKFIELKNTTVTEEGVKPPAPEAPAAEAAPVETADGAAAPT
                     ATEGAQPAPAAAEEAPAEAAPAPAAPAEAR"
     gene            complement(8225..9349)
                     /locus_tag="PKH54_02905"
     CDS             complement(8225..9349)
                     /locus_tag="PKH54_02905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98865.1"
                     /translation="MAEPILKLDRYVLLEEIGTGGMATVYKAKNTGPMGFEKLAAVKL
                     LHPDFGAGSDVVRMFIDEARIGARLSHPNVIGVTDFGQIENHYFMAMEFVEGCNLSTL
                     IKPGAARAARPLPLSAAACVMCDVLQGLEYVHAVKDEKGRSLGIVHRDISPQNIMIDR
                     SGRAKIGDFGIATGQFRESFTNAGIVKGKVSYMSPEQAAGKPLDGRSDIAAAGLTLFA
                     ALSGQSAFGTGDTIAVLARAEEGLKPESVKALECPDEIREIILKATCARIQDRFQTAR
                     EFREALAAAVPDFDGHGRELLAGMVTRASKAPSGRKKAKEQTAPVPGPEPRPTASSML
                     SRSMPSVPVSGLTIQTYRVLTGAVLLASIVALLLAMPWSA"
     gene            9500..>9944
                     /locus_tag="PKH54_02910"
     CDS             9500..>9944
                     /locus_tag="PKH54_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98866.1"
                     /translation="MRGFPKFAVSTFVAGLVISQCAFAASEPDAFDVVVNEGKKLSSQ
                     GRYVDAALVFERVLSQGNESQAAYQEAQYEMGVALFLLGLPVAAFTYFDRVGEVGESH
                     VRYIETLPWLVKVHQTLPGETSSLLRMASYPPAAYPPESADEISFN"
BASE COUNT         1901 a         2912 c         3079 g         2052 t
ORIGIN      
        1 gacctgtcgt caaggactgg cagaccgcgc tcccgggtct ggtcgcgcca ggccgagttg
       61 atctcgctga actgcctggt cggaacgagg aagaaggttc gccgcgactt gtccgcaagc
      121 atcgaggtga cctcggatct cgaagccttt tcctcgatgt tggccgtgta gaaattggag
      181 tcttccgaac ctctggagga gaaggttccg tgcctgaaca gggggcccgc ttccttccgc
      241 gcggcgtcct tccatgtatc gacgatgtgc ttctgggaaa ggtgtcggga aagttccggc
      301 atgaccgaga agatctgcac cgcggtgaag ccggcgatgc acgcgatgaa cagcggacgc
      361 tgaaccttga gtgtctggaa atcaaaaaac gcggattcaa gccgcgaaga ccatttggat
      421 ccgatcacag aggatgcgcg ggcccagatg agatcccacc agtggatcgc gaagacgatt
      481 ccaagaagca ggtatagtgc caggatgtcg gggccggtca gaaagatgcg cagccactcg
      541 gatccggcgc caggtttggt cagcgccggc catttcaggg cgatgaatac gatgatgtcg
      601 atcaggatga gtccgccggc cgtggcgacg gcggcaatga aagccttgcg cccggccatg
      661 aacgatttga cgagcaccgc gatgttcgac acgcgggatg cgaccgaaag gatggaaatg
      721 agcatcaggg caagcagaag ctttccgata gtcgggatct ggactgtcga ggggaatcct
      781 gcgtcgccct gtccaggctt cgagaacatc ggatcggtcg taaggaaagt caccagcggc
      841 gccggagact tcagcatgtc cttcatgatt acaaggaaaa ggccggccgc gaagaagccg
      901 aaaatccttg ccgccaggac gccgtcccga tccctgtctt caaaatcacg gaagaagagg
      961 gcggtcagca tggccatgcc cgggagtccg agataggacg tctggccgaa cggtctgatg
     1021 aacaccatcg cggtgaccat cgggacgacg gcccacagca gcaggagcat gcgggccggt
     1081 ctgccagtgt cggaacccgc ggcggccatc cagattgcca caggaatgaa ggcgctccac
     1141 gggaagaatc cgaatccgat ctccttgacg ataaaatcaa acgtcctgga ttccaggctc
     1201 atgccgcccg tgaaggtggc ggcggtgaat ctgaactgcc tgaaccaggc ggcctccgga
     1261 atgaatgtgg cgatggccag gacgcccaga agggcaaaga cggcgacaac cgccggaaga
     1321 tgacggcgat ccggggatac cgcgaaccat gtgcccgcga tcgcggcggc cgtaaccacg
     1381 gccgtcattc cgcccgacag ccagcacagc acaagtccgg ccgtcatcag cacccagggg
     1441 ccccacttgc ggacaggaca tttttcgtcc tcgcagggca tgacgatctg gccgaatgaa
     1501 agcgcggtca gggccacgaa gaatacttcg gccatgccgg cgccggcaaa tcgcgcggtt
     1561 ccgtaaaaca gcaggcttgt ggcaaggacg aacaatgcgc cggcgggaat ggcgtccgaa
     1621 aaaaggcgcc gcgtcccgaa caccagcagg gccagtgtca gcacggcgaa aagaacgcca
     1681 gtcaggcgcg cgctgaactc gttgtaaccg aaaacgtcga accagagcga ggtgatcatc
     1741 ggctcaagcg gcggaaggaa cgacgtcctg ccccccgact tgaactgtgc ggtcgaaccg
     1801 aagcaggagg cggcttccgt cacaagtcgc ttcagttcgg cactggagcc aatcgatacg
     1861 gcggtgagcg cgcgttcgag atcgaccgga gcatcgtcct cggtcgccgc gcccttgatg
     1921 accgcgtccc tgactgcggc ggcgtcaacc ccttcaaggc cgacaagtat gaatgaagtt
     1981 gaaaggttgc gtgaagccgc gcttcgaatg gcattcgcaa gggtcttcac ggcggccgga
     2041 tccgttccgg gcgcggcgac atctggaacc agcgcggacg ggtcgataat aaccgccgcg
     2101 aagatacgat cagacgtcct gtccctgaga gcgtccaggc cgcgagccga gccgccggcc
     2161 acgtcggcgg attcaacctt gactccgtca atcccgacgg cgtcgcgaat ggcgtggcca
     2221 ccttcctttt cgattacgag gattggcggt tcgccgctca ttcgccaggc aaccgcggag
     2281 tggttcatct cccacgggtc ccacaggcca agggaagccg tgcccgcgaa gagcacaagc
     2341 gcagagaaaa caatggcgga aatggaaatt atccgtgttc tgccgatacg tgaattaacg
     2401 ggatgtccgg ccaacttgcc acctcgatct ggctgactca aggaaactgc ggtagagtac
     2461 ttgctgttgc ctttgttttc aatagtccgg gcctttttac aacaagcgaa aaagacagtc
     2521 atgataacaa gatccgcttc agccagggac gttccggtgt tggccggccg gtcggccgcg
     2581 ttagcgcttc cgcctgattt cccgagcctt tccgggaccg cccgccttga tgcaatactc
     2641 aagctcgctt ccccagggaa attcatttcg cgcctccggg aggatgagtt cgtcttcctc
     2701 gtcaacggga tcgggctgga ggacgctggc gtgctggtga ggtacgcgac cagcgcccag
     2761 cgcaagcttc ttgtcgatat ggattcctgg gaaggggatg agttcaggcc ggatcggttt
     2821 gacaggatac tggacatcgt caaggagcat ggactcgatt tcacggttga gatgctgcgg
     2881 gagcttgatc caggcatcct catcctgtct ctcgtgaagc gggcgaagtt tcatacggtc
     2941 gaggaggccg aggacatgga attcgcgccg gggacctggt tcatgacgcc tgacggcacc
     3001 ttcgtggtgg aatgcgcgga tgaagacgat gtgccggccg taagaaacct tatcgatctt
     3061 gtatatgcca tcggcgtggc ctacgcgcac tggttgatcc agggtggcag atgggaaacg
     3121 gtttcttcgg ttcaggagca gtctttcgag tatcggcgtt cgaggcttgc ggacaacggg
     3181 tttcccgagg aagaggatcc ttattcgata tacgagccgt tcgacctgga gtccttccgg
     3241 agtcgcgtgg ctggaacgga tcccggcgaa acgagtgccg gcacggcaag gggcgaaccc
     3301 ctggcgctga cggtcgcgga cactccgaag ggactttttt tctggaaagt catttcatcg
     3361 ccggcagccg ccgggcttga ccatgcggca atcctttccc agacgatgaa cctggtcaac
     3421 cgcgtcatgg gggcttcggt ccgcgatctt tccgaagtcg agtcgtgggg tccggtggcg
     3481 gcacacgcgc taaccgtggt ttccgtcggc ctcgaggccg tttcgcgcgg tgacgtggag
     3541 gtggcggcct ccgtcctgaa gaagtcggtt ccccttgaac tttttcgtgc cgggattgag
     3601 ttttccaggc cggtctcggt tctggccagg caggtggtcg cggacgtcgg cggccttgcc
     3661 tcgctgtccc tgttcggcga ttcggctgcc gcggatatcg atgcggcggc cgccttcccg
     3721 gccaagtgcc cgccgtcgat ttcgggttcg gacggggaat atgtcgattt ccagaattgc
     3781 ggccaggtca ttgcttcgat ttcaatgatg aagaagttca aggcggtggt gcagttttcg
     3841 aaggcggtac tgggcttcga tccatcaggg aaccattccg gcgatggtct tcttcatccg
     3901 ccgcggtttg caagcgttgt cgcgaccgcc tgggcccgcc aggtgctcga cggggcactg
     3961 tcgctggcgc cccttcgcgg agccgacctt gtcaggcttc gcgggattgc gttcgatgga
     4021 tcggtgatcc gtccaggtct gagactttcc gcaagtggcg aaggcgacga gtacgcgctt
     4081 gccgtcaggg aattcatttc ggaatcgctt gattcggtcg aggccgggct tggaggtctg
     4141 gttgacgccg atatggttga cccgaggctg attggcgatt gtctgcttat tgcggaatag
     4201 tgtgactgat tacttcgtgg ccatggcggg caggaacgtc tgcagttcct cgacccagta
     4261 gttggcccgc ttgttggccc tgtcggccgc cagcagcctt tccatttccg gcatgatcga
     4321 cttgtcgccg agccgccaga tggacagcag cacgaaacga cggaagtcaa cgtcgcgctt
     4381 gggatcgatc tgcgggtaca gctcaatcaa ggccttcagt gcggcggccc tggcttccgg
     4441 ttcggtgacg ccaccgagga gcagcgcggc cttctggccg acggtcgtgt tctcgtcctt
     4501 agtcatctgc acaaggcact cgacatcgaa cggcttgcac tgttcaagcg gcttcacggt
     4561 cgccaggaat gcctgggcct cggcgttgtc gccctggaag cgctccttca cgagatcgga
     4621 cttctcggct gcgagcatct ttttgaagac ggcatagttc tgccagtcga tgcccaggga
     4681 gatgaccttc acaagcggcg ccctgaagac ttcccgcttg gtttccttga aaattttcag
     4741 cagcggcggg ataccggcaa aaccgggaac cagcgcgagc gagcttgcgg tgtcaaggcg
     4801 ctgcttggca tcgttgtctt cgttggtgat cgtttccacc agggtgggga tgatttcctt
     4861 gttgcccacg ttccacaggg ccatcatgct catggtgatg cggttctgct gggcgatctg
     4921 ccaggttctg atcacgcgat catcaacgcc gaccgggggg ttgaggacgc tgccgagatc
     4981 cttcgcaagt gcaagcgcgg ccctttcgtc acgaagatcg ccaaggatct ggacaagttc
     5041 cgggccatcg aagatctgcc agtcgaaaat gcccatcttg ccggccattg cgttgatctc
     5101 ctcgatcaac ctgccgtagt tctggacgtc cttggtaagg acgccgacga gcggttcgac
     5161 cgcgggacgg ccgatttcgg ccagggcgcg acgaaccggg acataagcct ggcgaccgcg
     5221 ctgatccctg atgaacacgg ccttgataag ggcgccgatg gccttttcgg acttcatctg
     5281 gccgagcagc ttcgcggctt cggcgttgac ctcgatcttc tgaaggttcg gatcctcgag
     5341 gagcagcttg atgcacagat cctcgtgctg gatcagatta tccttgtcga ttttggccag
     5401 ggccttgagg gcattcatcc tggcgtcgag ggccttgttg ctttcgagga tttccttcag
     5461 tcccgcacgg aattccggac gggcgaaatc ctcggcgacg gcggcagcct gaaccgcggc
     5521 ctcgggatct gcgagtgcgg cgttcaccag ttcaagcgac gcctcggggg cgttgatctg
     5581 ctgaaccgca agaaggatgt cgtccttgta ctggctgcgc gggaaaatct cggccagggc
     5641 cgggatcgat tcagcgcact tcagctcgcc aagattctgg atggccttct tgccgttgac
     5701 cgggctcttg aggagtttca cccagccttt gcaagtcgtg ggcgttccac gggcgcagcc
     5761 ggtgaaagtc gtcaagccaa tggccaggac agcggtaaga acgacggcct taagcagatt
     5821 acgcatcaat aaccctccgt tgatgctgaa cttccatgaa atgaccatat tagaacaagg
     5881 tgcagtataa ttctgcccgt gaacagaggt caagcgatta tgcgtctgaa ttcattacct
     5941 gccctgatgc tgcttctggc gacttcctgc ggcgggtgga ccgatggcgg gcagcccgtc
     6001 ggaattggcg gcccgactgc gggaagtccc gagtacaggg cccttcttgg cggggacata
     6061 tgccgggcgg acagggattg cgtgacggaa atatgttctg acggcacgtg cattggattc
     6121 ctgatgattt cgtccgatgc cggcaggaac gatgttggag ccctcatccg ggagaggttc
     6181 ggtgaatcgc ccgcctcctt gtcgaggctt gcctccattg cctcgggaat gatcgaggat
     6241 ccgctgaccg aggtgtattt ccggggacgc tcggccgact tcttccggtt cctgccctgc
     6301 gccatgtcgc agccgatgct tagggacaag ctggcgagcc gggatgaacc ggtcagattc
     6361 tttgccgccc gtgtcctttc gttgtgcggt gatcagaatg gcagggagat ccttgacggc
     6421 tttctttcgc atccctcgga ggcaatcagg cgaatggcgg cggcggttca gtagtccatg
     6481 ccggccgcga tgatccgaag gcgttcgacc tcggcgtcgg gcagtggcga cggctgcgaa
     6541 agggttcggg caagaagcac tatcctgctg ttgtgttcga ggctttctag ccggtcgtat
     6601 gccatgaaag ggtcgcttcc caggcagacc gccccgtggc gctccatcat gattacgttg
     6661 tgatcgaccg ccagccttcc aacgtcctga gcgagctcga cggaagacgg agtcgcgtac
     6721 cgtccggtcg gcaccgaacc cagcgtgatg accgattctg gcaggaatgc aggttcaagc
     6781 gagaggccgg ccagagtgag cgcgaccgaa gccaccgggt gtgcgtgaac gacggcaccg
     6841 cagtccggac ggttcctgta gcacgcaagg tgaaccttga tctcgctgga aacctcgaga
     6901 tcgggatcga ccggccttcc gtccatcgac gcgaccacga gatcctcggg ccggatgaat
     6961 cccttgttca ccccggaagg cgtaatcaat atcagctcgt cggtaagcct gacgctgacg
     7021 ttgccctgtc caccggaaac caggccgcgg gcgtacatgc gtcggcaggt ttccacgatc
     7081 gcggcccttt ttccggccca gatcattcat cccccgcttt cgactgcggc cgtccagacg
     7141 cggtcgatgc cgcatcgagc ttccacgcgc ccgcctcacg aacgaaaaac acggggactt
     7201 ccgttccgtc gggagcgatg gtcagaacca gtgccgtatc gcctgaaatt ttttcggatg
     7261 caatccttga acgggaaaac ttctctatgg gggtcttttc accggaccat gcctctttct
     7321 ggattgacag aagagcttcg gcgaatggcc tggattgcgt ggagaagtga cttccaaagg
     7381 cagcgggatc cccgcggacg gccgcttcct caagttcgtt cagtactttc gacggagagt
     7441 tggaacaagc ggttgccaag cccgccgcga gcatcagaac taatgtggcg gggcggttca
     7501 tggcggcctt ccgaaaacga cggcaaccgg agggaattcc ggtgaaacag cagatcccga
     7561 tctggtgcga gaggggggac tcgaaccccc acaggaacta ccctactagc acctcaagct
     7621 agcgtgtctg ccattccacc actctcgcgt ggtcacaaaa gcgtgcgaat gctatcagtc
     7681 ggaaaattcg ttgtcaacgg gcttcagcag gtgcggcagg ggcgggtgcg gcttcggcag
     7741 gggcttcttc ggcggcggcc ggcgccggct gggcaccttc ggtcgctgtc ggggctgctg
     7801 cgccatcagc cgtttccacc ggggcggctt ccgcggccgg cgcttccgga gcgggcggct
     7861 tcacgccttc ttccgtcaca gtcgtgttct tcagttcgat gaacttgtcc agattcttca
     7921 ggtagtcatt gcactcgtcg tcggccgtca gtttgccggc cgcctcgcag gcaaccgttt
     7981 tcggggccct ttcgcagact tccgtggcga ggttgcggca cggattgcgg ccgcacccgg
     8041 cgatggccag ggaaatgaat gccgtaacaa cgacaacgat ggattttttc acgttggatt
     8101 cctccagtct tgacccaagt cttgaccaat gatcaacccg gcttcagggc cggacgcggt
     8161 gccgcccgcc cgtttcgccg ccgccagaca ttactacact tcgcttcaaa aggcttgaag
     8221 attatcaggc cgaccacggc atggccagca gcagcgcgac tatcgatgcc agcagcacgg
     8281 cccccgtcag caccctgtaa gtctgtatcg tcaatcccga caccgggacg gagggcatcg
     8341 acctgctcag catggatgag gctgtgggtc gtggttccgg gcccggaact ggcgcggttt
     8401 gttcctttgc cttctttcgc cccgagggtg ctttcgatgc gcgggtcacc attcccgcaa
     8461 gcaactcgcg gccatggccg tcgaagtccg gaaccgccgc ggccagggcc tccctgaatt
     8521 ccctcgcggt ctggaatcga tcctgaattc tcgcacaggt ggccttcagg atgatttccc
     8581 tgatttcgtc cgggcattcc agggccttga ccgactccgg cttcagtcct tcctcggccc
     8641 gcgccagaac cgctatggtg tcccctgttc cgaacgccga ctgtcccgaa agcgcggcga
     8701 agagcgtaag gccggccgca gcaatgtccg atcgtccgtc cagcggcttg ccggccgcct
     8761 gttccggact catgtacgaa accttcccct tgacgatgcc ggcgttcgtg aaagattccc
     8821 tgaactgccc ggtcgcgatt ccgaagtctc cgattttcgc cctgccggat cggtcgatca
     8881 tgatgttctg cgggcttatg tcgcgatgca cgattcccag cgagcgcccc ttctcgtcct
     8941 tgaccgcgtg gacatattcc aggccctgca gcacgtcgca catgacgcat gccgccgcgg
     9001 aaagcggaag cggcctggcg gcacgggcgg cgccaggctt tatgagtgtt gaaaggttgc
     9061 agccctcgac gaattccatc gccatgaaat agtggttttc gatctgtcca aagtctgtta
     9121 cgccgattac gttgggatgg ctgagcctgg cgccaatcct ggcttcgtcg atgaacatgc
     9181 ggacgacgtc gcttccggcc ccgaaatcag ggtgaagaag cttgaccgcc gcaagctttt
     9241 cgaagcccat cggtccggtg ttcttggcct tgtatacagt ggccattcca ccagtgccga
     9301 tctcctccag aagaacgtac ctgtccagct tcagaattgg ttccgccatg gttgccccgg
     9361 aagaatcgag tcactcacca cggattgtgc gtcggaacac ccgcatcagt caaagattcg
     9421 accgtcctcc gggttgacag tattgattga tctgcaataa tgctgacaac ccggtccttc
     9481 tattaagtgg ggtcttaaca tgcgcggttt tccgaaattc gcggtatcga cctttgtcgc
     9541 aggccttgtc atctcccagt gcgccttcgc tgcgtctgag cctgatgcct tcgacgtggt
     9601 cgtgaacgaa ggaaagaagc tttcctcaca gggacgctat gttgacgcgg ccctcgtgtt
     9661 tgaaagggtt ctttcacagg gtaacgaatc ccaggccgcc tatcaggaag cacagtacga
     9721 aatgggcgtt gccctgttcc tgctgggcct gcctgtggcg gcgttcacat acttcgatcg
     9781 tgtcggtgaa gtcggcgaga gtcatgtaag gtacatcgag acccttccgt ggcttgtcaa
     9841 ggttcatcag acccttcccg gtgagacaag ctcgctgctc aggatggctt cctatccccc
     9901 cgcggcctat ccgcccgagt cggccgatga aatcagcttc aacg
//
LOCUS       DAOPPA010000056         9881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7789_length_9881_cov_4.242011, whole genome shotgun sequence.
ACCESSION   DAOPPA010000056 DAOPPA010000000
VERSION     DAOPPA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7789_length_9881_cov_4.242011"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKH54_02915"
     CDS             <1..660
                     /locus_tag="PKH54_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98867.1"
                     /translation="WKAGVLDVVESGSNPLGTLISMNLPAVPSGAAIDVDGRIYVPLS
                     DGTLRRYQETTSVRLQEVWSTVIFAGVGQQAVNLLVDDEGLIIAVSGTGDVQVVKDME
                     GFGSVIATGSFGTPLAPLHPVLGDSGRVVASSAAGRIVSVLVDSVAGLVIQGLGFNVP
                     SPPASDMLLLEDVLMYVSETGSLMSWSYPAGPAGRWSRSGANQGSTGRTARPALLSVK
                     P"
     gene            657..3512
                     /locus_tag="PKH54_02920"
     CDS             657..3512
                     /locus_tag="PKH54_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98868.1"
                     /translation="MIRKGFPAFLPLIFLAGCGNAEVGDIVAMCLTVNDCHYLEIEDC
                     TMPDCVDGQCVLVADRNADGLACGTSNRCVKTPGRCETGVCVGARQEQCPGIPCMVGR
                     CDPETGSCAYTQAPEKSSCDVDGNPCTSDICEAGSCRKGPNSCPCLRVSDCPNPLDLC
                     AGVFTCRLNFCEIDEMTRVICPADSSCYTHACNSATGQCERTDMPDDTVCAQEGPCTL
                     QGRCGSGICVIPDRCDDGNQCTTDNCDSVSGACDHQPSDGACDDEDQCTGPDACVLGV
                     CRGQPVSCDDRNPCTIDSCDSVSGCVNEPDKAGTPCDSGDLCVVGSVCDENGVCGNGS
                     PKCDDQSDCTIDTCESGLCDFVPVQGACDDGNSCTLGERCLGGVCGEGRPVPGCCNFA
                     ADCDDNDQCTVSACVDSECVHQGEPVGLCGGNDGCAVAWCQADSGQCGQVASSFPLVI
                     SSWDFVRGDHLIGFGWGSPAGSIGSAGLYAVDDQAAVVFPRRIVPSGVLQSIIRLDSG
                     ECGNVTFSNQPAGLSCIQGPDGAIVLAAFSTDSVCDSDGRIGLQFIVAGGAVIRSADL
                     VLLAMSGCDVDGLRVEGTGSATDIAAASRGSLVALAFRKGSNMSLAATRTGGGTMAVD
                     VAQDPFGVVSGRFGATIVDAGDRWALAWGAADDRVWIATANDSGVVSVPRKIPDVASE
                     GEIHAWPSLAVDSAGRLALAMSYSQSSDFDIALGLFDKDLSLIRDFERVNESDLGTRT
                     KPVVVVGQNRGLIGWISGILDSTPQHMFSLRQLDEVLVVQGFEIPYGVQSTEISQISI
                     TFSVDRYFCVWARADGGIGGVVLDSGTLALKGSFEIPGPFRSRPQVVATTEGALVVSI
                     SGTGATASIIAQSVSVSGAVSEPFVVASPVLAGLTSFAVTSFGPVHHVIAYSDILSDE
                     PGIRIVPFSGKCPGGFISGNSVCTGIGEDGRAGGVLP"
     gene            3509..5782
                     /locus_tag="PKH54_02925"
     CDS             3509..5782
                     /locus_tag="PKH54_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98869.1"
                     /translation="MKGAILVNTIVASLLVACSQAGDTGVSGSACGRDRDCDDSIACT
                     ADRCVLTVDGIKRCDNVPDDSSCPPGQACLVESVMGAGCQDPDRLICSGRSDGSACTS
                     TNACATDSGECSGGVCVYPTRECPSRPCMEGTGCDRDTGTCGYSVMPDDTLCDADGDR
                     CTSDRCRGGICVEGPDECECSDDRPCMQPANLCLGVATCEDGRCVSTPVDCPSRNSDC
                     MQYFCVPATGLCASVVVNEGGSCSDGLACTGTGTCDSGACVAPPLVCPPRSCNEAACV
                     EPEGCVYSPIPGPCDDSDMCNGPDACDAGVCRPTGTPVVCSDHNECTTDSCIPSTGRC
                     LFVSIPKCCGNGVLEDGEQCDGPGLPGSGCTACMFDVASLDFTGTSPSWAWSDTTGSG
                     LVAWEMIDNEGGRGIAVRPMSGLGVLGETIPVPAGTLKGRSMSPVVVAAADGFILGAL
                     APDGPSFWLLGADGRPRIKAVVRNPWNQGIPSSRLVAAISPLRGIAAWSVQAPDGRQQ
                     LLYADIASGSGQILASGSQTLFTSDPGGIVVPGDACPISGGVMVLFTVRAEPGGSSVI
                     RQMAAILQAGSPTPVVFELATSVWDQSLVARCSATPDGKAMAVFPAIVTKPEQELAIE
                     SVVIDPLAGPNPVVRVLGASDMQEGTIFPFQSDLVAMTSGGYSYITTLVTPNLQSPTG
                     IVPVIIPIEPDGTSGAEPFRLPGPDQAFVGAITAGLAGSSVLSVFWVQTGELMSVNEK
                     GEVAGRLYQDLTGGTGH"
     gene            5779..6423
                     /locus_tag="PKH54_02930"
     CDS             5779..6423
                     /locus_tag="PKH54_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC98870.1"
                     /translation="MNLHFIGHACFLISSADGTRLLIDPFQPRAFNGRIRLEPHSGPV
                     DAVISTHSHLDHRHIDPSFGSPLVFEGPGQIHGIDIDCIKLPHGAPEGKDHGLVRAFR
                     INVDGISIVHLGDAGRIPSDAEMQALGRPDILLLPIGGRFSLDPIQAAQLVRSWRPAF
                     AVPMHYQDPLVDITLRPLSEFTDQFDGFVTVSGDFTINAVDRGRPPIILVIRRA"
     gene            complement(6436..6966)
                     /gene="hpt"
                     /locus_tag="PKH54_02935"
     CDS             complement(6436..6966)
                     /gene="hpt"
                     /locus_tag="PKH54_02935"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOC98871.1"
                     /translation="MDGIEKLDVLISAKDLKRRIGELGAEITRDYAGREPVIVCVLKG
                     ALFFMADLCREIDLPITIDTLGMSSYGDQTSSSGVVRITQDLSKPIVDRDVIIVEDII
                     DTGLTLAFLLDNLRARKPRSIEICSLLHKPARTRIPVQAKYIGFTIEDRFVVGYGLDW
                     EQKMRNVPFVGVVRDA"
     gene            7157..7432
                     /locus_tag="PKH54_02940"
     CDS             7157..7432
                     /locus_tag="PKH54_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC98872.1"
                     /translation="MTKSELIEVVARTSDITLDRATIVVNAVFEQMVEAMKRDERIEI
                     RNFGNFTVKKYEGYEGRNPKTGAKVKVPQKRLPFFKAGLGLRDEINE"
     gene            complement(7444..8082)
                     /gene="coaE"
                     /locus_tag="PKH54_02945"
     CDS             complement(7444..8082)
                     /gene="coaE"
                     /locus_tag="PKH54_02945"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOC98873.1"
                     /translation="MHIMSKDLHSRAWGLTGGIGSGKSYAAAILRGLGIPVLDMDVMA
                     RRLMLAGSPVFDQVVRRFGPLIVGQDGQIDRKALAALVFTSQERRLELEGIIHPVVIR
                     QAFEMVDSAPLSKAPTIFIESALIFAVGLERSLARTVLVRAPQDVRIMRVMIRDKISE
                     QEVRARISAQMDEDAMQNRADIVWDNPDGSTGLRRQIILTLASQEAERYRGT"
     gene            8227..9201
                     /locus_tag="PKH54_02950"
     CDS             8227..9201
                     /locus_tag="PKH54_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC98874.1"
                     /translation="MIEVRRLVKDYGPLRALDGISFSVGAGEVAGFLGPNGAGKTTCM
                     KIVTGFISATSGSVAVNGLDTVDQGVEARRLMGYLPENAPVYGEMTVGEYLTFIARVR
                     MIPKRSIARRVMEVAETTGVQHRLNQETGTLSRGFRQRTGLAAALIHNPQILILDEPT
                     SGLDPNQIVEIRSLIRDIGRDRTVILSTHNLAEVTAVCNRIIIVHLGRIVADGTCDAI
                     SRDHGHADSCLVSFLTDGLPDHGEVAADLGRLEGCMDVTPVRDGVRQQLTFRLRAESG
                     RDLRPAIFSLAAARNWGLVELRSERLDLETIFSRLTRQATWAGDRWEE"
     gene            9189..>9881
                     /locus_tag="PKH54_02955"
     CDS             9189..>9881
                     /locus_tag="PKH54_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC98875.1"
                     /translation="MGRVIAIARREFFAHFGSPAAWVAIAIFLSLLGVTFFFNMPFFI
                     VKPAFFLAREASLRWLFEWMMFLFTIILPAISMRLLAEERKLGTMEILLTLPVDEFDV
                     VVGKLVGSIGFLLVALSLTLFYPLMLVILGQPDPGPILGGYIGVTLAGAAYLAIGLLA
                     SSWTSSQITAFIIAIAICAGMSFADRIPEALGLHSLPLLDAFSFGSHFQSIARGVIDT
                     RDILYFLGVLFTA"
BASE COUNT         1752 a         2839 c         3059 g         2231 t
ORIGIN      
        1 tggaaggccg gcgtgcttga cgtcgtggag tccggctcga atccgcttgg caccctgatt
       61 tcaatgaatc ttcccgccgt gccgtccggc gcggcgattg acgttgacgg ccgcatctat
      121 gttccacttt cggacgggac gttgcgcagg taccaggaaa cgacatcggt aaggttgcag
      181 gaagtgtggt caacggtcat ctttgcgggc gtcggacagc aggcagtcaa tcttctggtt
      241 gacgacgaag gcctgatcat agccgtttcg ggaaccggcg atgtgcaggt cgtcaaggac
      301 atggagggtt tcggttccgt tattgcaaca ggttccttcg ggacgccgct ggcgcccctt
      361 catccggtgc tgggcgattc cggccgcgtc gtcgcgtcct cggcggccgg ccgcatcgtt
      421 tccgtgctgg tggattccgt tgcggggctg gtgattcagg gccttgggtt caatgttccg
      481 tcgcccccgg catccgacat gcttctgctt gaagacgtgc tgatgtatgt gtccgaaacc
      541 ggaagtctga tgtcctggtc ctatccggcc ggtccggccg gcaggtggtc caggtcaggc
      601 gccaaccagg gctcgaccgg gcgaaccgcg cggccggccc tattgtcggt gaaaccttga
      661 tccggaaagg attcccagcg tttctgccac tgattttcct ggccggctgc ggcaacgcgg
      721 aagtcggtga catcgtcgcg atgtgcttga cggtgaatga ctgtcactat cttgagattg
      781 aagactgcac catgccggac tgtgttgacg gtcaatgcgt gcttgtggcc gatcgcaacg
      841 ccgatggact tgcctgcggc acatcgaacc ggtgtgtaaa aacccccgga cgatgcgaga
      901 ctggcgtctg cgtcggcgcc aggcaggaac agtgccccgg cataccgtgc atggttggca
      961 ggtgtgatcc tgaaaccggg tcgtgcgctt acacccaggc gcccgagaag tcgtcctgcg
     1021 acgtggatgg caacccgtgc acttccgaca tctgcgaggc cggatcctgc aggaagggcc
     1081 cgaattcatg cccatgcctt cgcgtatcgg attgtccgaa tcccctggat ctttgtgcgg
     1141 gagttttcac gtgtcgtctg aatttctgtg aaatcgacga aatgaccagg gtcatctgtc
     1201 cggcagattc cagttgttac acgcatgcct gcaattccgc gaccggacag tgcgaacgaa
     1261 ccgacatgcc tgatgacacg gtctgcgccc aggaaggccc ctgcacgctt cagggacggt
     1321 gcggcagtgg catctgcgtg attcccgaca ggtgtgacga cgggaaccag tgcaccaccg
     1381 acaactgcga ttcggtcagc ggcgcctgcg accaccaacc gtccgacggt gcctgcgatg
     1441 atgaagacca gtgcaccggg cccgacgcgt gcgtcctcgg ggtctgccgt gggcagcccg
     1501 tctcgtgcga cgatcgcaat ccgtgcacca tcgactcatg cgacagtgtg tccggttgtg
     1561 tgaatgaacc ggacaaggcg ggaaccccat gtgattcagg cgatttgtgc gtggtcgggt
     1621 cggtttgcga cgaaaacggc gtctgcggaa atggcagccc gaagtgtgac gatcaaagcg
     1681 actgcaccat cgacacctgt gagtccggcc tgtgcgactt tgtgccggtt cagggcgctt
     1741 gtgacgacgg caattcctgc acccttggtg aaaggtgcct gggcggcgta tgcggggagg
     1801 ggcgtccggt tcccggttgt tgtaattttg ccgccgactg cgatgacaat gatcaatgta
     1861 ccgtttcggc atgcgtcgat tcggaatgcg tgcatcaagg tgagccagtt ggcctttgtg
     1921 gtggaaatga cgggtgtgcc gttgcctggt gccaggccga ttccggtcag tgcggccagg
     1981 tcgcttccag cttcccgctg gtcatttcgt cctgggattt tgtccggggc gatcatctga
     2041 ttggattcgg ctggggaagt ccggccgggt caattggatc ggctgggctg tacgcggtcg
     2101 acgaccaggc cgcggtcgtg ttcccgcggc ggattgtgcc gtcaggtgtt cttcagtcga
     2161 ttatcaggct tgattcgggg gaatgcggca acgtcacttt cagtaaccag cccgccgggc
     2221 tttcgtgtat ccaggggccg gacggggcaa tcgttcttgc cgcgttttcg accgacagcg
     2281 tctgtgattc cgacgggcga atcgggcttc agttcatcgt ggctggcggt gcggtgatcc
     2341 gttcggccga ccttgtgctt ctggcgatgt ccggctgtga cgtggacggc ctgcgtgttg
     2401 aaggaacggg ctcggcaacc gacattgcgg ccgccagccg cgggagcctg gtcgcgctgg
     2461 cgttccgcaa gggatccaac atgtcgctgg cggcaaccag gactggtggc gggacaatgg
     2521 ccgttgacgt cgcgcaggat ccgttcggcg tggtttccgg caggtttggc gcgacgatcg
     2581 tggacgcggg ggatcgatgg gccctggcct ggggtgcggc cgacgacagg gtctggattg
     2641 ccaccgcgaa cgattccggg gtggtgtccg tgccacgaaa gatccccgat gtcgcgtcag
     2701 agggcgagat tcacgcatgg ccatccctgg cggtggattc ggccggccgg ctggcgcttg
     2761 ccatgtctta cagccagtcg tcggacttcg acatcgccct tggtctgttt gataaggatt
     2821 tgtcgttgat acgtgatttc gaacgtgtca atgaatctga cttgggaaca agaactaaac
     2881 ctgttgttgt tgtcggccaa aatcgaggtc ttatcggttg gatttcgggc attttggatt
     2941 cgactcctca acacatgttt agccttagac aacttgacga agtattggta gtgcagggat
     3001 ttgagattcc atatggagtc cagtccaccg aaatttcaca aatctctatc acattctccg
     3061 ttgaccggta tttctgtgtc tgggccaggg cggacggcgg gattggtggc gttgtgcttg
     3121 attccggaac cctggcactg aaaggttcgt ttgaaattcc cgggccgttc agatccaggc
     3181 cgcaggttgt cgcgacaacg gaaggcgcac ttgtcgtctc gatttcaggt actggtgcga
     3241 ccgcgtcgat tatcgcccag tcggtctccg tttccggggc cgtctcggaa ccgttcgtcg
     3301 tggcatcccc ggttcttgcc ggcctgacat cgtttgcggt tacgtccttc gggccggtgc
     3361 atcacgtgat tgcgtattcc gacatccttt cggacgagcc cggaatcagg attgttccgt
     3421 tttccgggaa atgtcccggg ggcttcatca gcggcaacag cgtctgcacg ggaattggcg
     3481 aagatggaag ggccggcggg gtgctgccat gaaaggcgcc atccttgtaa ataccattgt
     3541 ggcttcgtta ctggttgcct gttcccaggc cggcgatacc ggggtgtctg gaagtgcctg
     3601 tggccgtgat cgcgactgtg acgattccat cgcatgcacg gcggatcggt gcgtgctgac
     3661 ggtcgatggc atcaagcggt gcgacaatgt cccggacgat tcgagttgtc ccccggggca
     3721 ggcctgcctg gtcgagtcag tcatgggtgc cggttgccag gatccggatc gcctgatctg
     3781 ttcgggacgt tccgatggat ctgcctgcac gtcgaccaac gcatgcgcaa cggatagtgg
     3841 cgagtgttcc ggcggtgtct gcgtgtatcc gacaagggaa tgtccttcgc gaccgtgcat
     3901 ggagggtacc ggttgcgacc gggacactgg aacctgcggg tattcagtca tgccggatga
     3961 caccctgtgc gatgccgacg gggatcgatg tacctcggat cgctgtcgcg gcggcatctg
     4021 cgtcgaaggt ccggacgaat gcgaatgctc ggacgaccgt ccgtgcatgc agcctgccaa
     4081 tctgtgcctt ggggtcgcga cctgtgaaga tggccgttgt gtttccaccc cggttgactg
     4141 tccatccagg aattcggact gcatgcagta cttctgcgtg ccggcgaccg ggctttgcgc
     4201 aagcgtggtg gtcaatgaag gcggatcgtg cagcgacggg cttgcctgta ccggcaccgg
     4261 cacgtgcgat tccggcgcgt gcgttgcgcc cccgcttgtt tgcccgcccc gttcctgcaa
     4321 cgaagctgcc tgtgtcgagc cggaagggtg cgtgtactcg ccgatccctg gaccatgtga
     4381 cgacagcgac atgtgcaacg gtccggacgc ctgcgacgcc ggcgtgtgca ggccgactgg
     4441 aacgcctgta gtctgttccg accacaacga gtgcacgaca gattcgtgca tcccatccac
     4501 aggccgctgc ctgttcgtgt cgattccgaa gtgctgcgga aacggggttc tggaagacgg
     4561 ggaacaatgc gatggccccg ggcttcccgg cagcggctgt accgcctgca tgttcgatgt
     4621 ggccagtctt gatttcacgg gcacctcacc ctcctgggcc tggtccgata cgaccggttc
     4681 aggcctggtt gcctgggaaa tgatcgacaa cgaaggcggt cgcggcattg ccgttcgtcc
     4741 aatgtccggc ctgggggttc tgggcgaaac gatcccggtt cccgcgggaa ccttgaaggg
     4801 gcgttcgatg tcacccgtcg ttgtggccgc cgccgatgga ttcatcctcg gagcgcttgc
     4861 accggatggt ccgtcgttct ggctgctggg cgccgacggg aggccgagaa tcaaggccgt
     4921 tgtaagaaat ccatggaacc agggaattcc atccagcagg cttgttgcgg caatttcgcc
     4981 gctgcgcggg attgcggcat ggtcggttca ggcgcccgac ggtcgtcaac aactgctgta
     5041 cgccgacatc gcatccggtt ccgggcagat ccttgcgtcc ggttcccaga ccctgttcac
     5101 atccgatcct ggggggatcg tcgtacccgg ggacgcctgc ccgatatcgg gcggggtcat
     5161 ggtgttgttc accgttcgcg cggagccggg cggcagttcg gttatccggc agatggcggc
     5221 catcctccag gcaggctcgc cgacgccggt cgttttcgag cttgcaacgt cggtctggga
     5281 tcagtcgctg gttgccaggt gctcggcgac accggacggc aaggcgatgg cggtttttcc
     5341 ggccatcgtc acgaagccgg aacaggaact ggcaattgaa agcgtcgtca tcgatccgct
     5401 ggcgggcccg aacccggtcg tccgcgtcct gggcgcttcc gacatgcagg aaggcaccat
     5461 tttcccattt cagtccgacc tggtcgcaat gacttccggc gggtattcgt acatcacgac
     5521 cctggtaacc cccaatcttc agtctcccac cggaatcgtg cccgtgataa tcccgatcga
     5581 acctgacgga acatccggcg cggagccttt ccgactgccc ggccccgatc aggcatttgt
     5641 gggcgccata acggccggcc tggcagggtc gtcggtgctt tctgttttct gggtccagac
     5701 cggcgaattg atgagcgtca atgaaaaggg cgaggtcgcc ggccgcttgt atcaggatct
     5761 taccgggggg acagggcatt gaaccttcac ttcatcggcc acgcatgctt tctgatttca
     5821 tccgccgatg gaaccaggtt gctgatcgat ccgttccagc cgcgagcctt taacggaaga
     5881 atccgccttg aaccccactc gggcccggtt gatgccgtca tttcgacaca ttcccacctg
     5941 gatcaccgcc acatcgaccc gtctttcggc tcgcccctgg ttttcgaggg ccccggccag
     6001 atccatggaa tcgacatcga ctgcataaag ctgccccacg gggcgcctga aggaaaggat
     6061 cacggccttg ttcgggcctt caggatcaat gttgacggta tatccatcgt tcatctgggg
     6121 gatgccgggc ggattccatc agacgccgaa atgcaggccc ttggacgccc agatatcctt
     6181 ctgttgccga tcggcggaag attttcgctt gatccgatac aggcggcgca actggtcaga
     6241 tcctggcggc cggcattcgc ggttccgatg cattatcagg atccgcttgt cgatataacc
     6301 cttcggccac tgtccgaatt tacagaccag ttcgatggat tcgtgacagt ttccggtgac
     6361 ttcaccataa acgccgtcga tcgcggccga ccgccgatca tcctggtaat tcgaagggcc
     6421 tgattccggg cctggtcaag cgtccctgac gacgccgacg aacggcacgt tgcgcatttt
     6481 ctgttcccag tccagtccat acccgacaac aaatcgatcc tcgatcgtga atccgatgta
     6541 tttcgcctga accggaatcc tggttcgcgc cggcttgtgc agaaggctgc aaatttcaat
     6601 cgatctcggt ttccgggccc gcaggttgtc aagcaggaag gccagggtca gcccggtgtc
     6661 gatgatgtct tcaacgatga tcacatcgcg atcgactatg ggctttgaaa gatcctgcgt
     6721 gattcgaaca accccggacg atgatgtctg gtcgccatag gacgacatgc ccagcgtatc
     6781 gattgtgatt ggcaggtcga tttcacggca aaggtcggcc atgaaaaaga gagctccctt
     6841 aagcacgcag acgatgaccg gttcgcggcc ggcgtaatcc ctggtaattt ccgccccaag
     6901 ctcgccaatg cgccttttca ggtcttttgc ggaaataagc acgtcgagtt tctcgattcc
     6961 gtccatttcg gatcaccttc tgtccgcccc ggtcattttc attcggcccg gtcaggatgt
     7021 aaacgccagt tttcgcagca gtgacgggcg aaatttggaa agagggataa atactttgta
     7081 ataattacaa aatcttcttg acccacggtt gattttggct aacattacgc acgttttgaa
     7141 cctttggagg tcaataatga caaagtccga acttattgaa gttgttgcaa gaaccagcga
     7201 tattaccctc gaccgtgcaa ccatcgtcgt caacgcggtt ttcgagcaga tggtcgaagc
     7261 catgaagcgc gacgagcgca tcgagattcg taattttggt aacttcaccg tgaagaagta
     7321 cgaaggctac gaaggtcgca acccgaagac cggcgccaag gtcaaggttc cccagaagcg
     7381 tctgccgttt ttcaaggccg gccttggact ccgcgacgag atcaacgagt aaccggtcac
     7441 ccatcaggta cctcggtacc tttcagcttc ctgactcgcc agagtcaaga ttatctgcct
     7501 tcggagtcct gtcgagccgt ccgggttgtc ccagacgatg tccgcgcggt tctgcatcgc
     7561 gtcttcatcc atctgggccg aaattcttgc ccttacttcc tgttcggaaa ttttgtccct
     7621 gatcatcacc cgcatgattc gaacatcctg cggtgcccga accaggacgg tgcgcgccag
     7681 tgaccgttcg aggccgaccg cgaagatcag ggcgctctct atgaaaatgg tcggggcctt
     7741 cgacaggggt gcggaatcga ccatttcgaa tgcctgccgg atcacgacag gatgaatgat
     7801 gccctcgagt tcgagacgtc tttcctggct ggtgaagacc agagccgcca gtgccttcct
     7861 gtcgatctgg ccgtcctggc cgacaatcag cggcccgaac cgcctgacga cctggtcaaa
     7921 taccgggctc ccggccagca taagtcgcct ggccatgacg tccatgtcca ggacgggaat
     7981 tccaagcccc cggagtatgg ccgcggcgta agactttccg gaaccgatgc cgccagtcag
     8041 cccccaggcc ctgctatgaa ggtcttttga catgatgtgc atgatctgaa cttcgattgc
     8101 gcgcgatgtc aactcggata agctgcccgc gccctgttca caaagcggtt tcgggaatag
     8161 aatcatgcgt cggtcggttt tcggcgcggc ggtttgtcag gttcgccatc atgtgttggt
     8221 cacccaatga ttgaagtcag gcgcctggta aaggattatg gcccgcttcg ggcccttgac
     8281 gggatttcgt tttcggtcgg ggccggggag gtggccggtt ttcttgggcc gaacggtgcc
     8341 ggcaagacca catgcatgaa gatcgtgacc gggttcattt cggccacttc cggaagcgtg
     8401 gccgtcaacg ggctggatac ggttgaccag ggtgtcgagg caaggcgtct tatgggatac
     8461 ctgccggaaa acgcgccggt ctatggcgaa atgacggttg gagagtacct gaccttcatc
     8521 gcgcgcgtcc gcatgatccc gaagcgctcg atcgcgcgcc gggtgatgga ggtcgccgag
     8581 accaccggtg ttcagcaccg cctcaaccag gaaacgggaa ccctttcgcg cggattcagg
     8641 cagcgcaccg ggcttgcggc ggcgcttatc cacaatccac agattctgat cctggacgag
     8701 ccgacgtcag ggctggatcc gaaccagatc gttgaaatca gatcccttat cagggacatt
     8761 gggcgcgaca gaacggtaat actttcgacc cataacctgg ccgaggtgac cgccgtatgc
     8821 aaccgcatca tcatcgtcca tctggggcgc atcgtggcgg acggcacttg tgatgcaatc
     8881 agccgggatc atggccacgc ggattcgtgc ctggtgtcgt ttttgaccga cggtctgccc
     8941 gatcacgggg aagtcgcggc cgatcttgga cggctcgagg gctgcatgga cgtcaccccg
     9001 gtcagggacg gtgtcaggca gcagttgaca ttccggcttc gcgccgagtc aggtcgggat
     9061 ttgcgccccg ccatattctc gcttgccgcg gcacgcaact gggggctggt cgaattgcgc
     9121 agcgaaaggc tcgacctgga aacgatcttt tcccgcctga cgcgccaggc gacatgggct
     9181 ggtgacagat gggaagagtg attgccattg cccgccgtga gttttttgca catttcgggt
     9241 cgccggccgc ctgggtggcg attgcgatct tcctgtcgct tcttggcgtc acatttttct
     9301 tcaatatgcc gtttttcatt gtcaagccgg ccttcttcct ggcacgcgag gcgtccttga
     9361 gatggctttt cgaatggatg atgttccttt tcaccatcat cctgccggcc atttcaatgc
     9421 gtcttctggc cgaggagcgc aagctgggaa cgatggagat cctgcttacc ctgcctgttg
     9481 atgaattcga tgtcgtcgtc ggcaagcttg tcggttcgat cgggttcctt cttgtggcct
     9541 tgagcctgac ccttttctat ccgttgatgc tggtcattct tggccagccc gatccggggc
     9601 caatcctggg cgggtatatc ggggtgacgc tggcaggcgc ggcgtatctg gccattgggc
     9661 ttctggcctc gtcctggacg tcatcccaga tcacggcatt cattattgcg atagccatct
     9721 gcgccggcat gtcgtttgcc gacaggattc ccgaggcgct cgggcttcac tcactgccgc
     9781 tgctggacgc cttttcgttc ggtagtcatt tccagtccat agctcgtggc gtcatagaca
     9841 cccgcgacat actctatttc cttggtgtcc tcttcacggc c
//
LOCUS       DAOPPA010000057         9861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7808_length_9861_cov_3.901183, whole genome shotgun sequence.
ACCESSION   DAOPPA010000057 DAOPPA010000000
VERSION     DAOPPA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7808_length_9861_cov_3.901183"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKH54_02960"
     CDS             <1..785
                     /locus_tag="PKH54_02960"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOC98876.1"
                     /translation="AIAGYEGDPDAIDPALVKVDNVAAVVVQTPNFLGVIEDVRGFSD
                     MIHDAGGLSIVTFTEALAFGLIDAPGNRGADICAGEGQSLGIPLSFGGPYIGMMGVKQ
                     EFVKSMPGRLIGKTKDHEGRDCFVLTLAAREQHIRRERASSNICSNEALCAVALTIYM
                     SMLGRNGYPRLAMLNHLKAERLKGMLREAGFELPFAGPTFNEFVVRIGGDATRKIREL
                     AEQGMMAGIALGSYFPHLSDCILVAVTEKTRDADMDALIAAL"
     gene            858..1340
                     /locus_tag="PKH54_02965"
     CDS             858..1340
                     /locus_tag="PKH54_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98877.1"
                     /translation="MNRTLVPVTVIVAGLLAGACQEMEDFGECLMTQQMLSDCNAGLP
                     GTTDQCSTEDAFCGPTCIVANHPECTSGPCLTIRYKDILADKTWVSSPFCGSSCLTDT
                     DCPGEPGQAACKPMPGLKIECELDEDCQARADWSFCGTDKFCSWHVCVPINFAKANQE
                     "
     gene            1389..1868
                     /locus_tag="PKH54_02970"
     CDS             1389..1868
                     /locus_tag="PKH54_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOC98878.1"
                     /translation="MRTARLFMIIAALPLVACAARQIPGTAVDDTPANRDIVEVIEKY
                     RKACEKKDIDGILEVVSRNYFSNFGTTSNTDDDYGYEQLVKNMLPILRDEIKAVSYMV
                     YVKKISFPRENVAWADVEHSYKFYYVEQGKDRWKPGTNFKRFEFAKEDGVWRITSGL"
     gene            1965..4487
                     /locus_tag="PKH54_02975"
     CDS             1965..4487
                     /locus_tag="PKH54_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC98879.1"
                     /translation="MKRVVVVGGVAGGMSAAARVKRLDPTAAVTVFEQDGDISFANCG
                     MPYYIGGVIQDRNQLLVQTPESMKARYGIDVRIRHRVTAIERIAKTVTVLDRDRNVEF
                     TEPYDALILSPGAFPIKPPLPGIDRPDVFILKNLDDMDRIRQAAVKAKRAVVVGAGYI
                     GVEVAENLRHAGVIVDIVERLDQMIAPLDPEMTRQLYDEARLNRVKVHLSTQVTSFDD
                     DGVHLSDGGVIPADFTVMSVGVKPVSGLAAQAGLKLTATGHIMVDEHMRTSDPAIFAV
                     GDAVAAFSQKTGGYIANPLAGPANRQGRIAADNICGRDSVYGGTQGTSIVKIFNLAAA
                     GTGMNAKALKAAGVKGFHTAWIHPNQHPGYYPGAAPIDIKVIFDDEGRILGAQAVGRE
                     GVDVVINSLAQAMRGGQTVFDLEEVELAYCPAWGNAKHPINMVGFVAANILRGDMEVI
                     EPWEKPDAWLDVREADETAAGMIPGAIHIPMGQIESRLAEIPRDSSIGVYCAVGLRGY
                     IVYRRLKNLGFKVRNLNGGYRTWRWFNVTDEVADQPLLTRAPVAEAGVALPGQAASGQ
                     PGAAARQSSDPVMPDRTVKLDVCGLQCPGPLMKVRKVMDDMKEGEVLEVTASDPGFAA
                     DIPMWTRQTGNTLLDVTSDAGLYRAVVMKSGRAAAAPRQGSVADSGVSVSASPHAAGG
                     PTAKGKTVIVFSQDLDKVLASFIIANGAAAMGAPVTMFFTFWGLNVLRRPETVQVKKT
                     FTERMFGSMMPRGAGRLKISNMNMGGMGTAMIKRIMKDKNVLSLDELIGSARDSGIRL
                     VACAMSMDLMGIKAEELIDGVEIAGVGNYLGTADEGNVNLFI"
     gene            4617..7214
                     /locus_tag="PKH54_02980"
     CDS             4617..7214
                     /locus_tag="PKH54_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98880.1"
                     /translation="MRRLVLLSTIAVTAALAACGSSGDPIEEDTGINEVFQDSGSDVG
                     IDTQTGDDAIIDSGTDNAWMDVHLPDVKEDIPEGPFYAKEEGDLVTVSDGRLTVVLNL
                     ADGTFNIGFTPGLDGIINGHSEAWVRSSEGVTRISSMNPAQRAAWQAMQTTDGLDDGV
                     LIGMETVPSGSIGGMRTFIALHRNQPSVTIKVQLVLPVREGFFIEKLVPVVARGSEGA
                     GMLLGPGTATARIVTGGHEIAYDANAEAINVTEAVETLNKPGYLSDNATLICTKPGKC
                     LLAGFLNTVRGFGGVAVATDPVSAFAVGEDAALSLFEMRSTMVQGAMLSPSRVITSEV
                     AYLDFTEEPGPALARFADAIATFNTLPVPRALPFMYSARVGQEGLTSDQVVAAIPDDS
                     EGVTGFLVEQGWENAEKAPDAVKFPNHGGTGAMKWIADQAKAAGLVPGIALGPFVFKN
                     DSAFAAAHAEWLLEPGELAVVRLGIGNNSSILDLTSDDARAWAVAHVTTAAGDWGYRY
                     IKIHGADIACMAKPGSTTGMTGVEGLRKFLEEVRAAVGADVFIELADGGMIGLGLVDA
                     IGTADPQYKSWNAPASKVDNTLQMGAMTWARRAWLDGTVFNVVPGPAFDFSQDGTLTA
                     SGWIGPRLWAVTGGVPTIAEAQGIQGLLAELAGNPFGDAWAGDYADLRIPETWQAGYE
                     RDGVASFLAGFFRFEGNMDLYTLAPVNGARTRTLQLPQAEGNWLAIDPETAEMATVDG
                     ACMNPAGMELVLESGNSASRVLVLRKISAGGAPTFMAITPSIAGGDGLVSQLSSDAGS
                     TTVTYDAFFRGDTTVVFAVPVANSPTVSVEGGTIIDQGVSDTACSDMKTAWIKFTPTA
                     DSVTVTASN"
     gene            7251..8903
                     /locus_tag="PKH54_02985"
     CDS             7251..8903
                     /locus_tag="PKH54_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOC98881.1"
                     /translation="MSAKLLIRDLTLRDGQQSQFATRMNQVQVDRVLGLFREAGFHAM
                     EVWGGAVPDSVMRYLGEDPWTRLESIKAVIGDRSSLTALSRGRNLFGYNPYPDSVIEG
                     FCRNAVQAGIGIMRIFDALNDVDNMRSSIRFVKDAGGRADCAVCYTVDPKFTLAARIR
                     ATVSGHPLPRKVFGVDYFVDKAVALQEAGADMISIKDMAGLVDPATAGLLIPALKKRL
                     DIPIDMHTHCTPGFGVATCLMAMVNGCDMIDTVCLPFSGGPAAPAFEIVELFARKLGL
                     ETGVDAAVVTRINRELTEVRRELAEFDQFKLFPREFDLSRDNLPDDIDALFDSAVDAA
                     RGSKIDRLLDFCHRIEAWFNYPPPDEVVRVAQIPGGMYTNMLAQMKSANLSVHMKEVM
                     QTVPSVRLAAGLPPLVTPTSQIVGVQAVMCVINRHKGVPVWSNPSSQFVNLVKGSYGR
                     TPVPVDPDFRQMLAGVREETPWDTSGYKPQENPELPEFGGVRLAANSREELLLELFPN
                     VAGPFLKGVRKAEFEAERRRLGEIESMANAGLFTAIGGNYLA"
     gene            complement(8891..>9861)
                     /locus_tag="PKH54_02990"
     CDS             complement(8891..>9861)
                     /locus_tag="PKH54_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98882.1"
                     /translation="SPARLESAGCKVRDWWAGVLRRPARALLPIGLMVAVSIAAALAA
                     TRLPPPLGWISGALNVFDDAWSTVPAFLARFNSVSLILLACAGVAASILKPLRFLMLP
                     FWAIFLMRIHVEGTDDRFNHWMRLFSLDMPAVMIIAVFGLKLIRDRWVPAGVHPHLRA
                     LAVAALAWSVLILPAGSDNPGRYPFRMGHPGDAGNHANPQIEVNYLLRIMDRYPDCLF
                     ATRVLDHGEFKDVHFSRTLVVTDEPLVGFFDCQLFYRGLDCNFVDGPHCTDNTEDMQP
                     LDSLSFDDLPYGQIRPYLGRQHRPVIELGVFGLRSGGALSGAGQAR"
BASE COUNT         2041 a         2950 c         3037 g         1833 t
ORIGIN      
        1 ccgccatcgc cggctacgaa ggcgaccctg acgcgatcga ccccgccctg gtcaaggtcg
       61 ataatgtcgc cgccgtggtc gtccagacgc cgaatttcct gggcgtcatc gaggatgtcc
      121 gcggattttc cgacatgatt cacgatgctg gcggccttag catcgttacg ttcaccgaag
      181 ccctcgcttt cggccttatc gatgcccccg gaaaccgcgg cgcggatatc tgtgccgggg
      241 aagggcaatc cctggggatc cccctgtctt tcggcggtcc gtacatcggc atgatgggcg
      301 tcaagcagga attcgtgaaa tcgatgcctg gaaggctcat cggcaagacg aaggatcacg
      361 agggccgcga ctgcttcgtc cttacgctcg cagcacgtga acagcacata cggcgcgaaa
      421 gggccagttc gaacatctgc agcaacgagg cactctgcgc ggtggccctg acgatctaca
      481 tgtcgatgct gggcagaaac ggatacccgc gcctggcgat gctgaaccat ctcaaggcag
      541 aaagactgaa gggaatgctt cgcgaagccg gctttgagct tccgtttgcc ggtcccacct
      601 tcaatgaatt cgtcgtccgg attggcggcg acgcgacccg taagatccgg gagcttgccg
      661 agcagggaat gatggccggc atcgcgcttg gctcttactt cccgcacctg tccgactgca
      721 tccttgtcgc ggtcaccgaa aagacccgtg acgccgacat ggacgcactg attgccgccc
      781 tttgatggcg tttggtattg actggcggtc ggcctgctat acaatctatc gggtgcattc
      841 catcggagga tcccgtgttg aatcgaactc tggttcccgt cactgttatt gtggccggtc
      901 ttctggctgg cgcgtgccag gagatggaag acttcggcga atgccttatg acccagcaga
      961 tgctgtctga ctgcaacgcc ggacttcccg ggacaaccga ccagtgctcg accgaggacg
     1021 cgttctgcgg cccgacctgc atcgtggcca atcacccgga atgcacatcg gggccgtgcc
     1081 tgaccatcag gtacaaggac atcctggccg acaagacctg ggtaagctcc ccgttctgcg
     1141 gcagctcgtg cctgactgat accgactgcc ccggcgagcc cggccaggcg gcctgcaagc
     1201 cgatgcccgg cctgaagatt gagtgcgagc ttgacgaaga ctgccaggcc cgcgcggact
     1261 ggtcgttctg cgggacggac aagttctgct cgtggcacgt ctgtgtcccg atcaattttg
     1321 ccaaagcgaa ccaagaataa ttttcacgct ggccacgtta tccgaccagg tatcccggag
     1381 gactcccgat gcgaaccgca cgcctgttca tgattattgc cgcccttcca cttgtcgcat
     1441 gtgccgccag gcagatcccc ggaacggccg tcgatgacac tccggccaac cgcgacatcg
     1501 tcgaggtcat cgaaaaatac cgcaaggcct gcgagaagaa ggacatcgac ggcatcctcg
     1561 aagtggtgtc gaggaactac ttctcgaatt tcgggacaac ttcaaatacc gacgacgatt
     1621 atggatacga gcagctcgtc aagaacatgc ttccaatcct tcgtgacgaa atcaaggccg
     1681 tgtcctatat ggtgtacgtc aagaaaataa gtttcccgcg tgaaaatgtt gcatgggccg
     1741 acgtcgaaca ctcgtacaag ttctactatg ttgaacaggg caaggatcgc tggaagccgg
     1801 gaaccaattt caagcgcttc gaattcgcga aagaagacgg cgtgtggcgg ataacgtccg
     1861 gcctgtagtg attcccgcgt caggcagcac gacaagaatc cggacaggat tgaaataaca
     1921 gcggtggaca aaccgtcggg ttcgtccgcc atcatggagg caaaatgaaa agggtcgtcg
     1981 tggttggtgg agtggccggc gggatgagcg ccgcggcaag ggtcaagaga cttgatccga
     2041 cggcggcggt caccgtcttc gagcaggatg gcgacatatc cttcgccaac tgcggaatgc
     2101 cctattacat cggaggcgtc atccaggaca ggaaccagtt gctggtgcag actcccgaat
     2161 cgatgaaggc gaggtacggc atcgatgtcc ggataaggca tcgcgtcacg gcaatcgaaa
     2221 ggatcgcaaa aaccgtgacg gtcctggatc gcgaccgcaa cgttgaattc accgaaccat
     2281 acgacgcact gatcctttcg cccggggctt tcccgatcaa gcccccgctt cccggcatcg
     2341 acaggccgga cgtattcatc ctgaagaacc ttgacgacat ggatcggatc cggcaggcgg
     2401 cggtcaaggc aaagcgggcc gtcgtcgtcg gagccggtta catcggagtc gaggtggctg
     2461 aaaacctgcg ccatgcgggt gtcatcgtgg atattgtcga gaggctggat cagatgatcg
     2521 cgccgcttga cccggaaatg accaggcagc tttatgacga ggcgcgcctt aaccgcgtaa
     2581 aggttcatct ttcgacccag gtgacatcgt tcgatgacga cggggtccac ctcagcgacg
     2641 gtggggtgat cccggccgac ttcaccgtca tgtcggtcgg cgtgaagccg gtatccggcc
     2701 ttgccgccca ggctggactt aaactgaccg caaccgggca cataatggtc gatgaacaca
     2761 tgcggaccag cgacccggcc atattcgctg tcggcgacgc ggtggcggcg ttctcgcaaa
     2821 agaccggggg ctacatcgcc aaccctctgg caggcccggc caaccggcag ggaaggatcg
     2881 ccgcggacaa catatgcgga cgagacagcg tctatggcgg aacccagggc acatccatcg
     2941 tcaagatatt caacctggcc gccgccggaa ccggcatgaa cgcaaaggcg ctgaaggccg
     3001 ccggcgtgaa ggggttccac accgcctgga ttcatccgaa ccagcaccca gggtattacc
     3061 cgggggcggc cccaatcgat atcaaggtga tattcgacga tgaaggccgg atccttggcg
     3121 cgcaggccgt ggggcgggaa ggcgtcgatg tcgtcatcaa ttcgctggcc caggccatga
     3181 ggggcggcca gaccgtcttc gacctcgagg aggttgaact ggcatactgc ccggcatggg
     3241 gcaacgcgaa gcatccgatt aacatggtcg ggttcgtcgc ggcaaacata ctccgtggcg
     3301 acatggaagt tatcgagccc tgggagaagc ccgacgcatg gcttgacgtg cgcgaggcgg
     3361 acgagactgc ggcaggcatg attcccggcg cgattcacat cccgatgggc cagatcgaat
     3421 caaggcttgc cgagattccg cgggacagct ccatcggggt ttactgcgca gtcggccttc
     3481 gcggctatat cgtctatcgc cgcctgaaga accttggatt caaggttcga aaccttaacg
     3541 gcggatacag gacctggcgc tggttcaacg ttacggacga agtcgccgac cagcctctcc
     3601 tgacccgggc acctgttgcc gaggccggtg tcgccctgcc cggccaggcc gcttccggtc
     3661 aaccgggggc agccgcccgg cagtcatccg acccggtgat gccggatcgc acggtcaagc
     3721 tggacgtgtg cggacttcaa tgtccagggc ccctgatgaa ggtccggaag gtcatggacg
     3781 acatgaagga aggcgaggta ctcgaggtga ccgcgtccga ccccggattc gcggccgaca
     3841 tcccgatgtg gacgcggcaa accggaaaca ccctgcttga cgtcacctcg gatgccggtt
     3901 tgtacagggc cgtcgtcatg aagtcaggca gggccgcggc cgcgccacgc cagggttccg
     3961 tcgcggattc cggcgtgtcg gtttccgcat ccccgcacgc ggccggagga ccgaccgcga
     4021 agggcaagac cgtcatcgtg ttttctcagg atctggacaa ggtccttgca tcgttcatca
     4081 tcgcgaacgg ggcggccgcg atgggcgcgc cggtcaccat gttcttcacg ttctgggggc
     4141 tgaacgtgct gcgaaggccc gaaacagtcc aggttaaaaa gactttcacc gaacggatgt
     4201 tcggttcgat gatgcccagg ggcgctggac gattgaagat ttccaacatg aacatgggcg
     4261 ggatgggaac cgcgatgatc aagcgaataa tgaaggacaa gaatgtcctt tcgcttgacg
     4321 agttgatcgg atcggcccgc gattcgggca ttcgcctggt ggcctgcgcc atgtctatgg
     4381 acttgatggg aatcaaggcc gaggaactga ttgacggcgt ggagattgcc ggagtcggca
     4441 attaccttgg aactgccgac gaaggcaacg tcaacctttt catctaggct gccgccccac
     4501 tcacgccggc ggcgcgcaag ttccggctcg attgtctttt gacctccatg cgtagtagaa
     4561 tcccgcgggt tcatttgtat tcgggcctgg attcacatag tcctggaggt atcgtgatgc
     4621 gaagactggt tctgttgagc accattgccg ttaccgccgc cctggccgcg tgcgggtcgt
     4681 ccggtgatcc gatcgaggaa gacaccggaa tcaacgaagt attccaggac tccggcagcg
     4741 atgtcggaat cgacacgcag accggagatg acgcaatcat tgattcgggt accgacaacg
     4801 catggatgga cgtgcatctt cccgacgtga aggaagacat cccggaagga cctttttacg
     4861 cgaaggaaga aggcgatctg gtcaccgtgt ccgatggaag gctgaccgtg gtcctgaacc
     4921 ttgcggacgg cacgttcaac atcggcttca ctccgggcct ggacggaatc atcaacggcc
     4981 attccgaagc ctgggttcgc agcagcgaag gcgtgaccag gatatccagc atgaatccgg
     5041 cccaaagggc tgcgtggcag gccatgcaga cgaccgacgg ccttgatgac ggcgtgctga
     5101 tcggcatgga aaccgttcca agcggctcga tcggcggcat gcgcaccttc atcgcactgc
     5161 accgcaacca accttccgtc acgatcaagg ttcagcttgt cctgccggtt cgggaaggct
     5221 tcttcatcga aaagctggta cctgtggtcg ccaggggttc cgaaggcgcc ggaatgcttc
     5281 tgggcccggg aaccgcaact gccaggattg tgaccggcgg ccatgaaatc gcctatgacg
     5341 ccaacgcgga agcgattaac gttaccgagg ctgtcgagac attgaacaag cccggatacc
     5401 tttccgacaa cgccacgctc atctgcacta agcccggcaa gtgccttctt gcgggtttcc
     5461 tgaataccgt ccgcggcttc gggggcgtcg ccgtcgcgac cgacccggtt tccgccttcg
     5521 cggttggcga agacgccgcg ctgtctctgt ttgagatgcg cagcacgatg gtgcagggcg
     5581 ccatgctcag tccgtcacgc gtcatcactt ccgaggtcgc ctacctcgat ttcacagagg
     5641 aaccggggcc ggcgcttgcc cgcttcgccg acgctattgc gactttcaac acgcttcccg
     5701 ttcccagggc actgccgttc atgtattcgg ccagggtcgg ccaggaaggc ctgacctccg
     5761 atcaggtcgt tgcggccatt cccgacgatt ctgaaggcgt gaccggattc ctcgtcgaac
     5821 agggctggga aaatgcggag aaagctcccg atgcggtcaa gttcccgaac catggtggaa
     5881 caggcgcgat gaaatggatt gccgaccagg caaaggccgc cggcctggtt ccggggatcg
     5941 cgcttggccc gttcgtcttc aagaatgatt ccgcctttgc cgccgcccat gcggaatggt
     6001 tgctcgaacc tggtgagctt gctgtggtca ggcttggaat cggaaacaat tccagcatcc
     6061 ttgaccttac gtccgacgac gcaagggcat gggccgtggc gcacgtgacg actgccgcgg
     6121 gggactgggg ttaccggtat atcaagatcc acggcgccga tatcgcctgc atggccaaac
     6181 cgggatcgac gacgggaatg accggcgtcg aagggctgcg caagttcctc gaggaagttc
     6241 gcgccgccgt cggggccgac gtcttcatcg agcttgccga tggcggaatg atcgggcttg
     6301 gcctggtgga tgcaatcgga accgccgacc cgcagtacaa atcatggaat gccccggcgt
     6361 cgaaggttga caacacgctg cagatgggcg cgatgacctg ggcccgtcgg gcatggcttg
     6421 acggcaccgt attcaacgtg gtgcccgggc ctgccttcga cttcagccag gacggcaccc
     6481 tcacagcttc cgggtggatt gggccccgtc tctgggccgt caccggcgga gttccgacca
     6541 tcgccgaagc ccagggcatc caggggctgc ttgccgaact ggccggcaac ccgttcggag
     6601 acgcatgggc cggcgactat gccgatctgc gcattcccga aacctggcag gccgggtacg
     6661 aaagggacgg cgtcgcatcc ttccttgccg gattcttcag gttcgaaggc aacatggatc
     6721 tttacacact cgcccccgta aacggggccc ggacacgcac gctgcagctt ccccaggccg
     6781 agggcaactg gcttgcgatc gaccccgaaa ccgctgaaat ggcgacggtc gatggcgcat
     6841 gcatgaaccc cgcgggtatg gaactggttc tggagagcgg aaattccgcc agccgcgtcc
     6901 tggtgctgcg caaaatctcg gccggcggcg ccccgacctt catggccatc acgccatcga
     6961 ttgccggggg tgacggtctg gtaagccagc tcagctcgga cgccggctcg accactgtaa
     7021 cctatgacgc cttcttcagg ggcgacacga ccgtcgtgtt cgcggttccg gttgccaaca
     7081 gcccgaccgt atcggtcgaa ggcggcacaa tcatcgatca gggggtctcg gatacggctt
     7141 gcagcgacat gaagacagcc tggatcaagt tcaccccgac cgccgattcc gtcacggtga
     7201 ccgcctccaa ctgatttttg ccccgtttcc cggggcggga cttgattgaa atgtctgcaa
     7261 aattgctcat tcgggatctt acccttcgcg acggtcagca gtcccagttt gccacccgca
     7321 tgaaccaggt tcaggttgac agagtgctcg gactgttccg ggaggccggc tttcatgcca
     7381 tggaagtttg gggcggtgcc gtccccgatt cggtgatgag atacctcggg gaggatccct
     7441 ggacccgcct ggaatcaata aaggccgtca tcggcgaccg ttccagcctg accgccctgt
     7501 cgcggggacg caacctgttc gggtacaatc cttatccgga ctcggtcatc gagggcttct
     7561 gccgcaacgc agtccaggcc ggaatcggga taatgcgcat cttcgacgca ctgaacgacg
     7621 tcgataacat gcgcagttca atccgtttcg tgaaggacgc cggagggcgt gccgactgcg
     7681 cggtctgcta cacggtcgat ccgaaattca cgcttgccgc cagaatccgg gcgacggttt
     7741 ccggccaccc gcttccccga aaggtgtttg gagtcgacta cttcgttgac aaggcggtcg
     7801 ccctccagga agccggcgcc gacatgattt ccatcaagga catggccggg ctggtcgatc
     7861 ccgccactgc cggccttctg attccggcac tgaagaagcg cctggacatc ccgatcgaca
     7921 tgcacacgca ctgcacgccc ggattcgggg tcgcgacgtg cctgatggcg atggttaacg
     7981 gctgcgacat gatcgatacc gtctgtctgc cgttttcggg cggaccggcg gcccccgcgt
     8041 ttgagatagt tgaacttttc gcgcgcaagc ttggcctgga gaccggagtc gatgcggccg
     8101 tggtcaccag gatcaaccgg gaattgaccg aggtcagaag ggaactggcc gagttcgacc
     8161 agttcaagct gttcccgcgg gaattcgatc tttccaggga caatttgcct gacgatatcg
     8221 acgccttgtt cgattcggct gtcgacgcgg cgcgcgggtc aaaaattgac cggcttctgg
     8281 acttctgtca ccgcatcgag gcgtggttca attatccgcc gccggacgag gtcgtgcggg
     8341 tcgcccagat acccggcgga atgtacacga acatgctggc gcagatgaaa tcggccaacc
     8401 tgtccgtcca catgaaggaa gtcatgcaga cggtgccatc cgtcaggctg gcggccggac
     8461 tgccgccgct cgtcactccg accagccaga ttgtcggcgt ccaggcggtc atgtgcgtca
     8521 tcaaccgtca caagggcgtg cccgtgtggt cgaatccttc gtcgcagttc gtcaacctgg
     8581 tgaagggaag ctacggcaga accccggttc cggttgaccc ggacttcaga cagatgcttg
     8641 ccggagtccg cgaggaaaca ccatgggaca cgtcaggata caagccgcag gaaaatccgg
     8701 agctcccgga attcgggggc gtccgacttg ccgcgaactc acgggaggaa ttgctgcttg
     8761 aactgttccc gaatgtggcc ggccccttcc tcaagggcgt caggaaggcc gagtttgaag
     8821 ccgagcgcag aagacttggg gaaatcgaat cgatggccaa tgccggactg ttcacggcga
     8881 taggcggcaa ctatctggcc tgacccgctc cggaaagggc gccacccgaa cgcaatccaa
     8941 aaacgccgag ttcaatcacc ggcctgtgct ggcgtccaag atacggacga atctgaccgt
     9001 aaggcaaatc gtcgaatgaa agcgagtcaa ggggctgcat gtcttcagta ttgtctgtgc
     9061 aatgcggtcc atcgacgaaa ttgcagtcaa gtccgcggta aaacagctgg cagtcgaaaa
     9121 agccgacaag gggctcgtcc gtgacaacga gtgtccggct gaaatggaca tccttgaact
     9181 ctccgtgatc gagcacgcgc gtcgcgaaca gacaatcggg ataacgatcc atgatccgaa
     9241 gcaggtagtt gacctcgatc tgcgggttgg cgtgattgcc ggcgtcaccc gggtgaccca
     9301 tccggaaagg gtatcgaccg gggttatccg aacccgcggg caggatcaga accgaccagg
     9361 ccagcgccgc gacggcaagc gcgcgaagat gcggatgcac gccggcaggc acccacctgt
     9421 cacgaatcag tttcaggccg aagacggcaa taatcatgac cgccggcata tcaagagaaa
     9481 acaggcgcat ccagtgattg aacctgtcat cggtgccttc cacgtgaatg cgcatcagga
     9541 atattgccca gaacggcaac atcaggaacc tgagaggctt cagaatcgag gccgcgacgc
     9601 cggcacacgc aagaaggatc agcgacacgg agttgaatct tgccaggaaa gcgggaaccg
     9661 ttgaccatgc atcgtcaaaa acgttcaggg cgcccgagat ccagccaagc ggtggcggta
     9721 gcctggtggc cgccagtgca gccgcaatcg aaactgccac catcaggccg attggcagca
     9781 gtgcccgcgc cggacgcctc aacacgccgg cccaccagtc tctgaccttg catccggccg
     9841 attccaggcg ggcgggcgac a
//
LOCUS       DAOPPA010000058         9788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_7872_length_9788_cov_3.911230, whole genome shotgun sequence.
ACCESSION   DAOPPA010000058 DAOPPA010000000
VERSION     DAOPPA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_9788_cov_3.911230"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKH54_02995"
     CDS             <1..1059
                     /locus_tag="PKH54_02995"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOC98883.1"
                     /translation="GFKPGIWTAPFSVSPDSQVFASHPDWIVRHENGKPVKAGFIMGR
                     FGPRPYYALDTTIPEVRDHIVTLYRRLRQMGWILFKVDFLTSAMVAGVRRDRTVTRAQ
                     AYRLGMQAVRDGIGDDGLMLTGIGPVLVNCGIIDIQRLSPDTCFGSPAWLTAEQKLTG
                     DRMTPGVRNQTATSIARTFLNDIVFSGDPDAIVGPGLKDNEAVYLKTVALMTGSTLTI
                     GHDFVKAGFDFSGYGPLLDVRGPAVAPDLGDAEHPRHLFVDTLFEGRPVRFYAILNPT
                     EQAELTPVRGDVLGGAVVQAVDYWAGQAVGLEAGRLLDLRPRSARLFMLDRGAAGAAD
                     TALPVQGGSDTTGRERME"
     gene            1056..1940
                     /locus_tag="PKH54_03000"
     CDS             1056..1940
                     /locus_tag="PKH54_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98884.1"
                     /translation="MNMSIHVRGILAVILVSLFASGGCVSYRHGWQMKPAQSFNGSPS
                     VMELAERMEQTAATDDQVEDLIDELKTIRRTYGENSALLTKLARSYLAMAVAYARDMP
                     EQGEFLRLSMMYSEAALMQNPRFAAMIQSMGPDHGTVAAAVAAGSVGSEEAWTLATWA
                     LASLMYYEECTSEVLKIRHRDTMLADIVTVINAISSVGGDEVKPLAFALKGVAVAVRP
                     KAKMVMAVPDFDAAVQAAPDSILVRWLQAKYLFANFGDKVSLAAAISQIKEIDLNSAG
                     GFKPLNRAIRVSLEKPKR"
     gene            2021..3421
                     /gene="asnS"
                     /locus_tag="PKH54_03005"
     CDS             2021..3421
                     /gene="asnS"
                     /locus_tag="PKH54_03005"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOC98885.1"
                     /translation="MKKYSVSEILRGVAPIGAEVTVQGWVRTRRDSGAGLSFIALHDG
                     SCFATLQLVAAAGLANYETDVMKLSAGCSVTVSGQVVKSAGKGQDVEVTVSSLTVVGW
                     VDDPETYPIQPKPHTLEFLREVAHLRPRTNIIGATMRVRDCLAQAVHRFFHENGYYWI
                     HTPIITASDAEGAGDMFRVSTLDMANLPRTEAGAIDWDQDFFGREARLTVSGQLNVET
                     YCLALSKVYTFGPTFRAENSNTRRHLAEFWMIEPEIAFADLAADAALAEAFLKYIFKA
                     VLEERADDLAFFAKFVDKTVISRLESFVSSEFAVMEYTDAIRYLKEAKVKFEFVPDWG
                     CDLQAEHERYLTETVVGRPVAVVNYPKDIKAFYMRMNDDGRTVAAMDILAPGIGEIVG
                     GSQREERLDVLDARIAEMGLNRDDYWWYRDLRRYGTVPHAGFGLGFERAVMYATGADN
                     IRDVIPFPRTPRNAKF"
     gene            complement(3425..5488)
                     /locus_tag="PKH54_03010"
     CDS             complement(3425..5488)
                     /locus_tag="PKH54_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98886.1"
                     /translation="MSSAIKSTLCLMALSSFMLVACGTQDVQNQDTLDPADTLQEDAG
                     TDSADGILVDTTGGSDADVIPTDQGGSDTDASASDDGNDATVVHGCHLEMIPRFIGGG
                     TQPVGFAKTVSVRLANTGTAACTVLSVATVDCPADGDGLPVCPDPLTAQPSTVFLIQT
                     PIEQIQVSIGPGSFKEIQISIEPEEDTPGVGPAGQLWGLLSVAFKDDASGAEKRLPEC
                     ATPCEPNMLAYMAGGQASALPGNIDFGLVASGCISKTNAVCVYNTGTSPISLESISLP
                     DCTDEFELDDVPALPLYVLAGQRTCFEAKYRPADEGLDTCSIQIVANGAADPVITIPL
                     RAKGTTIADQVDDFIQQRSDAVDILFVIDDSGSMCDEQAVLLESFGTFIERARTWNND
                     FHIGVISVNADDPDIAGHLNLGDRSVSPRFLTPSPSILELFNEVAAIGCDGSSSVESG
                     LHASLIALMPPLTTDTTVECNTNDTCTDNPIICPTPSTCQYTCVEQSCAGYNKEFLRP
                     DARLELIIMSDEDDQSPMPVADYVAMLEDLKAGFGPDMVGVNVIVGVEGVPLPGDEYS
                     ACTSPGGSTAVAGTRYIEAANMTDGVVASICQASFEDAMDDIGQKVFSDGGRLEYFLS
                     RLADESTISVSINRQDGPDFTACPTGWEYNAERNSIVINPEGDCVPQTDHTIRVQYQV
                     PCITE"
     gene            complement(5589..5885)
                     /locus_tag="PKH54_03015"
     CDS             complement(5589..5885)
                     /locus_tag="PKH54_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98887.1"
                     /translation="MDNRIAGKQFGHCVICGSTGKGENDFICSDCGDPLCATLYCTGC
                     GRRLNLDAKSARSFLADYNYDVKDTCGLIIKVDRCGACMALDETANIEIYRIHF"
     gene            6045..7439
                     /locus_tag="PKH54_03020"
     CDS             6045..7439
                     /locus_tag="PKH54_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOC98888.1"
                     /translation="MGIGGTAMSAVAGLLKDSGHEVRGTDRVYPYPPVGDLLAALGIT
                     VMYPYDAANLSWEPDLVVVGNVIRRDNPEAQAMREAGIPFMSFPQALKEHYLSGRVPL
                     VVTGTHGKTTTSSLVAWLLHAQGFDPGYLIGGVPLDFGSNYRAGRGSYFVVEGDEYDT
                     AYFDKGPKFLHYAPQAAIINNVEFDHADIYADLDAVIESFAKFARIMPSGAPLIVPEA
                     DANAAVAIRETCASVKTFGVDAGFWHARDVAWSNGRVGFDLMAGKTHAGRFDSPLFGR
                     HNLSNTVAALGLVHEAGAVGPGLADALSRFAGVKKRQEIKGVRGGVTVIDDFAHHPTA
                     VRETILAVRNAFPGARIVGMFEPESNTSRRKVFQEEYVTAFAAADRILFCAPLEKNDN
                     LPPEMKIDMGRLVADINRRGTPADIIPDIDELAAAAVASARPGDVILGMSGRDFYGVH
                     GKVLDLLERRDSTA"
     gene            7486..8277
                     /locus_tag="PKH54_03025"
     CDS             7486..8277
                     /locus_tag="PKH54_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOC98889.1"
                     /translation="MDVRQIDSDIGSRLRRDGTILEGEDLTVLADRWLFVQAVRGYRF
                     GIDAVLLARYASGLVGSHVPESASVLDIGAGCGVVGLILADLCPRVTVTGIEIQTAQA
                     DRARRNVALNGLEERVTIRTGDVREFAATGARFDLVVSNPPFYRAGSGRVNPDGERAV
                     ARHEISLDMDGLLEAVSRLMAPGGRAVVLYPAERLSECVLAAGRHGLAGVELVPLLPR
                     PGAQPESFLVGLAEGIDGSARDPVTAPPMCLSREAPPAATSECGR"
     gene            8356..>9788
                     /gene="metG"
                     /locus_tag="PKH54_03030"
     CDS             8356..>9788
                     /gene="metG"
                     /locus_tag="PKH54_03030"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOC98890.1"
                     /translation="MPESRPAYYVTTPIYYVNDRPHIGHAYCTILADVLRRYHSMFGC
                     ETFFLTGVDEHGQKVEEAAKKRGLTPQQHCDEMQAHFRDLWPTLDISNDDFIRTTEPR
                     HERVVKQALQQLFDSGDIYKGEYEGFYSQRVEKFFTEDELIDGKCPETGGEVQRIVEA
                     NYFFRMSRYQQRLIDHINANPDFIRPESRKNEVLGFLRKPLQDLCISRPKSRLSWGIE
                     LPFDSDYVTYVWFDALLNYVSVIGLFHDDPRFRKWWAVSTHLIGKDILTTHSVYWTTM
                     LFALGLDMPRHIIAHGWWLVDNTKMSKSLGNVINPLAMRDKYGVDSLRYFLMREMVVG
                     LDSDFSEESFLKRHNFELANDLGNLANRLVGFAAKQMGGVIPAPGTGSGHAADAEVMA
                     MAAELPARVRGHVESLRVETAIETVMNFVRRLNRYVAETEPFKVIKTDPAAAGGAVYI
                     ALEGLRIALNLLWPVMPKKVENLLGALG"
BASE COUNT         1857 a         2825 c         3119 g         1987 t
ORIGIN      
        1 ggtttcaagc ccggtatctg gactgcgcct ttctcggtat ccccggacag ccaggtattc
       61 gcttcacatc ccgactggat cgtgcgccac gaaaacggca agccggtcaa ggccggtttc
      121 attatgggcc gcttcggtcc gcgcccgtat tacgcactgg atacgaccat tcccgaggtt
      181 cgcgaccata tcgtgacgtt gtatcgcagg cttcggcaga tgggctggat tcttttcaag
      241 gtcgatttcc tgacgtcggc catggtggcc ggtgtccgca gggatcgaac cgtcacccgg
      301 gcccaggcgt acaggcttgg catgcaggcg gtccgggacg gcatcggtga cgacggcctg
      361 atgctgaccg gaatcggtcc ggtgctggtc aactgcggca tcatcgacat ccagcgcctg
      421 agtccggaca cgtgcttcgg ttcgcccgca tggctgactg ccgagcagaa gctgaccggc
      481 gacaggatga cgcccggcgt gcggaaccag acggcaacga gcatcgccag gaccttcctg
      541 aacgacatcg tgttttccgg ggatcccgac gcgatcgttg ggccgggcct gaaggacaac
      601 gaggccgttt atctgaagac cgtggccctg atgaccggaa gcacgctgac aataggtcat
      661 gacttcgtga aggccgggtt cgacttttcg ggatacgggc ccctgctgga cgtccggggc
      721 ccggcggtcg cgccggatct tggtgatgcc gaacatcccc ggcatctgtt cgtggatacc
      781 ctttttgaag gcaggccggt gcgtttctac gcgattctga atccgaccga gcaagccgaa
      841 ttgacgccgg tgcgcggcga cgtgcttggc ggcgcggtgg ttcaggcggt cgattactgg
      901 gccggccagg ctgtcggcct cgaggccggc cgactgctgg atcttcgtcc gcgcagtgcg
      961 cgcctgttca tgcttgatcg tggcgcggct ggtgctgccg acactgcttt acctgttcaa
     1021 ggcgggtccg ataccacggg ccgggagcgt atggaatgaa tatgtcgatt catgtgcggg
     1081 gcattctggc ggtgatcctt gtttccctgt tcgcttcggg cggatgcgtg tcgtacaggc
     1141 acgggtggca gatgaagcct gcccagtctt tcaacggcag tccatcggtt atggaactgg
     1201 ccgaaaggat ggagcagacc gccgcgacgg acgaccaggt cgaggatctg atagacgaat
     1261 tgaaaacaat tcgaaggaca tacggcgaaa acagcgcgct gctgacgaag ctggccaggt
     1321 cgtatcttgc gatggcggtt gcgtatgcgc gcgacatgcc ggagcagggc gagttcctgc
     1381 gtctttcgat gatgtattcc gaagctgccc tgatgcagaa tccacggttt gccgccatga
     1441 tccagtcaat gggtcccgac catggaaccg tcgcggcggc ggtcgcggcc ggctcggtcg
     1501 gttccgagga ggcatggacg ctggcgacct gggccctggc cagcctgatg tattacgagg
     1561 aatgcacgtc cgaagtgctg aagattcgtc atcgcgacac gatgcttgcc gatatcgtga
     1621 ccgtgatcaa tgccatctcc agtgtcggcg gcgacgaagt caagccgctt gccttcgcac
     1681 tgaagggcgt tgctgtcgcg gttcggccga aggctaagat ggtcatggcg gttccggatt
     1741 tcgatgccgc cgtgcaggcc gccccggatt cgatattggt tcgctggctg caggcgaagt
     1801 acctgttcgc gaattttggc gacaaggtca gccttgccgc cgcgatttcg cagatcaagg
     1861 aaatcgacct gaactccgcc ggcggcttca agccccttaa ccgcgccatc cgcgtatcgc
     1921 ttgaaaagcc gaaaaggtag gtcgggcggg cgccggttac atcgggcccg gaaccgtgta
     1981 caattccccg aagtttcttt gcaaccatcc aggtcttgaa atgaaaaaat actcggtaag
     2041 tgaaattctt cgcggcgtcg cgccgatcgg cgcggaagtc acggtacagg gatgggtcag
     2101 gacaaggcgc gattccggcg ccggcctgtc ctttatcgca cttcacgacg gctcgtgctt
     2161 cgcgacgttg cagcttgtgg ccgcggccgg gcttgccaac tacgaaaccg acgtcatgaa
     2221 gctgtcggcc ggctgttccg tcacggtttc cggccaggtc gtcaagtccg cgggcaaggg
     2281 acaggatgtc gaagtcacgg tgtcgtcgct gacggtcgtc ggatgggtcg atgatcccga
     2341 gacgtacccg atccagccga agccgcacac cctggaattc ctgcgggaag ttgcccacct
     2401 gcggccgcgg accaacatca tcggcgcaac catgcgcgtg cgggactgcc tggcccaggc
     2461 cgttcaccgg tttttccatg aaaacggcta ttactggatt cacacgccga tcatcaccgc
     2521 atcggatgcc gagggcgccg gcgacatgtt ccgcgtatcg acgcttgaca tggcgaacct
     2581 gccgcgcaca gaggcgggcg ccatcgactg ggatcaggat ttcttcggac gcgaggcccg
     2641 cctgacggtg tccgggcagc ttaacgttga aacgtactgc cttgcccttt caaaggtcta
     2701 tacgttcgga ccgacgttta gggccgaaaa ttccaatacc aggcgtcatc tggccgagtt
     2761 ctggatgatt gagcccgaga tcgcctttgc cgatctggcc gcggacgcgg cgcttgccga
     2821 ggcgttcctg aagtacatct tcaaggccgt gcttgaagag cgtgccgacg acctggcctt
     2881 cttcgcgaaa ttcgtcgata agacggtcat ttccaggctt gagtctttcg tttcgtccga
     2941 attcgcggtc atggagtaca ccgacgccat caggtacctg aaggaagcga aggtcaagtt
     3001 cgagttcgtc ccggactggg gttgcgatct gcaggccgag cacgaaaggt accttaccga
     3061 gacggtggtc ggccgtccgg tcgccgtggt caattatccc aaggatatca aggcgttcta
     3121 tatgcggatg aacgacgatg gccgaaccgt tgccgccatg gacatcctgg cgccaggcat
     3181 cggcgagatc gtcggcggat cccagcgcga ggagcgcctg gacgtcctgg acgcgcggat
     3241 tgcggagatg ggcctgaacc gggacgacta ctggtggtac cgggatctgc gccggtacgg
     3301 caccgttccg cacgccggat tcggcctggg attcgagcgc gccgtgatgt acgcgaccgg
     3361 tgccgacaat atccgggacg tcatcccgtt cccgcgaacc ccacgaaacg caaagttctg
     3421 attatcattc agtgatgcat ggaacctggt actgcacgcg gattgtgtga tcggtctgcg
     3481 ggacgcagtc gccttcggga ttgatcacga tcgaattcct ttcggcgttg tattcccatc
     3541 cggtcgggca ggccgtgaag tccgggccgt cctggcggtt gatcgagacc gaaatcgtcg
     3601 attcgtcggc cagcctggac aggaagtatt caagccgtcc accgtcagaa aacaccttct
     3661 gtccgatatc gtccatggca tcttcgaacg acgcctggca gatggatgca accacgccgt
     3721 cggtcatgtt ggcggcttcg atgtaccttg tgccggcgac ggcggtcgaa ccaccggggg
     3781 aagtgcacgc gctgtattca tcgccgggaa gcgggacgcc ctcgaccccg acaatcacgt
     3841 tgacgccgac catgtccggc ccgaatcccg ccttcaggtc ttcaagcatc gccacgtagt
     3901 cggcgacggg catcgggctc tggtcgtctt cgtccgacat gatgatcagt tcaagtcggg
     3961 cgtccgggcg gaggaactcc ttgttatatc cggcgcagct ctgttcgacg cacgtgtact
     4021 ggcaggttga aggcgtggga cagatgatcg ggttgtcggt gcaggtgtcg ttggtattgc
     4081 attcgaccgt cgtgtcggtc gtcagcggcg gcatcagcgc gataagggac gcgtgcagcc
     4141 cggattccac ggacgaactg ccgtcgcagc cgatcgccgc gacttcgttg aacagttcaa
     4201 gaatgctcgg cgacggggtc aggaatctgg ggctgacgct gcggtcgcca aggttcaggt
     4261 gaccggcgat gtccgggtca tcagcgttga cactgatgac cccgatatgg aaatcgttgt
     4321 tccaggtgcg cgcccgttca atgaacgtcc cgaaggattc aagcaggact gcctgttcat
     4381 cgcacatcga acccgaatcg tcgattacga aaaggatatc gaccgcgtcg ctgcgctgct
     4441 ggatgaaatc gtccacctga tccgcaattg tcgtgccttt cgcgcgcagc ggaatggtga
     4501 ttaccggatc ggccgcgccg ttcgcgacaa tctggatgct gcatgtatcc aggccttcgt
     4561 cggccggccg gtattttgcc tcgaaacacg tccgctggcc tgccaggacg taaagcggca
     4621 gcgccggcac atcatcaagc tcgaattcat cggtgcagtc cggaagcgaa atcgattcca
     4681 gcgaaatcgg cgaagttccg gtgttataga cgcacacggc gttcgttttt gaaatgcagc
     4741 cggaagccac cagtccaaaa tcgatgttgc ccggaagcgc gctggcctgg ccaccggcca
     4801 tgtacgcaag catgttcggc tcgcagggtg ttgcgcattc cggaaggcgc ttttcggcac
     4861 cggatgcatc gtccttgaag gcaaccgaca ggagtcccca caactggcca gccggtccga
     4921 cgccgggggt gtcctcttcc ggctcaatcg atatctgaat ttccttgaaa cttccgggcc
     4981 cgatggagac ctgaatctgt tcaatcgggg tctgaataag gaataccgtt gaaggctggg
     5041 ccgtcaacgg atcgggacag accggcagtc cgtcgccgtc ggccgggcaa tcgacggtcg
     5101 caacggaaag taccgtgcac gcggccgttc cggtgttcgc aagcctgacc gacaccgtct
     5161 tggcgaatcc gacaggctgg gttccgccgc cgatgaacct gggtatcatt tccaggtggc
     5221 atccatgaac gacggtcgca tcgttgccat cgtcggaagc agacgcgtcg gtgtcggaac
     5281 cgccctgatc cgtcgggatg acgtcggcgt cggatccgcc ggtcgtgtca acaaggatcc
     5341 cgtccgccga gtcggtgccc gcatcttcct ggagcgtgtc ggccgggtca agggtgtcct
     5401 gattctgcac gtcctgcgtt ccgcatgcca caagcatgaa ggacgacagg gccatcagac
     5461 atagcgtcga tttgattgcg gacgacatcg ggaaccccac gttaagtcaa agatggcaat
     5521 aactttatcc attttcggtg acggattcaa tatgactttt caagctatgt cggccggttc
     5581 cgcggtggtc agaagtgaat gcggtaaatt tcgatgttgg cggtttcgtc aagggccatg
     5641 catgcgccgc agcgatcgac cttgataatc agtccgcagg tgtccttgac gtcgtaattg
     5701 taatcggcca ggaacgaacg ggccgacttc gcgtccagat tcaggcgccg tccgcagccc
     5761 gtgcagtaaa gggtcgcgca cagcgggtcg ccgcagtcgg aacatatgaa gtcgttttcg
     5821 ccctttccgg tggatccgca gattacgcag tgtccgaatt gcttgccggc gattctgtta
     5881 tccaagaccg tctccgatga ggcccgatga cggacgaaga atacgttgtg gcggcaaaaa
     5941 ctgccaaatt gctgcttgat tttcgcaaat tagtatgatt cgggattcgg cgataagatc
     6001 cgcttcgacc ttacccgcaa ggcgccagga tgaaaattca tgtgatggga atcggaggaa
     6061 ctgccatgtc ggcggttgcc ggcctgttga aggattcggg gcatgaagtg cgcggtaccg
     6121 accgtgtcta tccctatcct ccggtcggtg acctgctggc cgcgctgggc atcacggtga
     6181 tgtacccgta cgatgccgcg aacctgtcct gggagcctga cctggtggtt gtcggcaacg
     6241 tcatacgccg ggacaatccg gaggcccagg cgatgcggga ggccggcata ccgttcatgt
     6301 cgttcccgca ggcgttgaag gagcattatt tgtctggccg ggttccgctg gtcgtgacgg
     6361 gcactcacgg aaagacgacg accagttcgc tggtcgcatg gctgctgcat gcccagggtt
     6421 ttgacccggg ttacctgatt ggcggcgtgc cgcttgattt cgggtcgaat taccgggccg
     6481 gccgcggcag ctacttcgtc gtggaaggcg acgaatatga cacggcatat ttcgacaagg
     6541 ggccgaagtt cctgcactat gcgccgcagg cggcgatcat caacaacgtc gagttcgacc
     6601 acgctgacat atacgccgac ctggacgccg tgattgaatc gttcgcgaag ttcgcgcgga
     6661 tcatgccgtc cggggcgccg ctgattgttc cggaagcgga tgcaaacgcc gcggtcgcga
     6721 ttcgcgagac atgcgcgtcc gtgaagactt tcggggtcga tgccgggttc tggcatgcac
     6781 gtgacgtggc ttggtcgaac gggcgcgtcg gattcgacct gatggccgga aaaacgcacg
     6841 ccggacgctt cgattcgccg ctgttcggac gccacaacct gtcaaacacc gtcgcggcgc
     6901 tcgggcttgt tcacgaggcc ggggcggtcg gcccgggcct tgccgatgcg ctgtcgcgct
     6961 ttgcgggggt caagaagcgc caggagataa agggggtaag gggcggcgtg acggtcattg
     7021 acgacttcgc gcaccatccg acggcagtcc gggaaacgat actggccgtt cgcaacgcct
     7081 ttcccggggc gcgtatcgtc ggtatgttcg agcctgaatc gaacacaagt cgccggaagg
     7141 tgttccagga agagtacgtg accgctttcg cggccgccga tcgcatcctt ttctgtgcgc
     7201 cgcttgaaaa gaacgacaac cttccgcccg aaatgaagat cgacatgggc cgactggttg
     7261 ccgacatcaa tcgtcgaggc acgccggccg acatcatccc cgacatcgac gagcttgcgg
     7321 ccgccgcggt cgcctcggcg cggcctggcg acgtgatact gggcatgtcg gggcgggatt
     7381 tttacggagt gcacggaaag gtcctggacc tgctggaaag aagggattcg accgcttaac
     7441 cgtcgccctg ggcggcggcg atcggaggcg gcaagggcca ttttgatgga cgtgcggcaa
     7501 atcgattcgg acatcggttc aaggcttcgc cgggacggaa ccatccttga gggtgaagac
     7561 ctgaccgtgc tggccgatcg gtggctgttt gtccaggcag tgcgcgggta tcggtttggc
     7621 atcgacgccg tgctgcttgc caggtacgca tcgggactgg tcggttctca tgtgcctgaa
     7681 tcggcttcag tcctggacat cggcgccggg tgcggtgtcg tcggcctgat tctggccgac
     7741 ctgtgtccac gggtcacggt gaccggaatc gagatacaga cggcgcaggc cgatcgggcg
     7801 cgcaggaacg tggccctgaa cgggcttgaa gagcgcgtga cgatacgtac cggcgacgtg
     7861 cgggagtttg cggcgaccgg ggcacgattc gaccttgtcg tttccaaccc gcccttttac
     7921 agggccgggt ccggaagggt caatccggac ggcgaaaggg cagtcgcccg ccacgagata
     7981 tccctggaca tggacgggct gctggaagct gtctccagac tgatggcacc aggcggccgg
     8041 gcggtggttc tttatccggc cgaaaggctt tccgagtgcg tcctggctgc gggccggcac
     8101 ggactggccg gcgttgaact ggtgcccctg ctgccgcgtc cgggcgccca gcctgagtca
     8161 ttccttgtcg gactcgccga aggcatcgac ggcagcgcgc gggatcccgt tacggcaccg
     8221 ccgatgtgcc tgtccaggga agcgccgccg gccgcgactt ccgaatgtgg ccgttaagga
     8281 ttacacttcc gcacgtcgcg ccggaaatca tgacttccgg ggcatttacg gatccattca
     8341 tccggagagg aaagcatgcc cgaatccaga ccagcttatt atgtaaccac cccgatttat
     8401 tatgtaaacg accggccgca catcggccat gcgtattgca cgatcctggc cgacgtgctg
     8461 cgacgttacc attcgatgtt cggctgcgag acctttttcc tgaccggcgt cgatgagcat
     8521 ggccagaagg ttgaagaggc cgcgaagaag cgtggtttga caccgcagca gcactgcgac
     8581 gagatgcagg cacatttccg ggatctgtgg ccgaccctgg acatatcgaa cgacgatttc
     8641 atcaggacga ccgaacccag acacgaacgc gtcgtcaagc aggcgctgca gcagcttttc
     8701 gattcgggcg acatctacaa gggcgaatac gaaggctttt acagccagcg cgtcgagaag
     8761 ttcttcacgg aagatgaact gatcgacggc aagtgcccgg aaaccggcgg ggaagtccag
     8821 cgcatcgtcg aggccaatta cttcttcagg atgagccggt accagcagcg cctgattgac
     8881 catatcaacg cgaaccccga cttcatccgc ccggagtcgc ggaagaacga agtccttgga
     8941 ttcctgcgca agccgcttca ggacttgtgc atcagccggc ccaaatcgcg cctgtcgtgg
     9001 ggaatcgaac tgccgttcga ttcggactac gtaacgtacg tatggttcga cgcgcttctg
     9061 aattacgtgt cggtcatcgg actgtttcac gacgatccgc gcttcaggaa gtggtgggcc
     9121 gtttccacgc acctgattgg aaaggacatc ctgacaactc actcggtcta ttggacgacg
     9181 atgctgttcg cgctggggct tgacatgccg cggcacatca tcgcgcacgg atggtggctg
     9241 gtcgataaca ccaagatgtc gaagtcgctt ggcaacgtga tcaatccgct tgcgatgcgg
     9301 gacaagtacg gcgtcgattc cctgcggtac ttcctgatgc gcgaaatggt ggtcggtctc
     9361 gattccgatt tttcggaaga gtcgttcctg aagcgccaca attttgaact ggcgaacgac
     9421 ctgggcaacc tggcaaaccg ccttgtcggc ttcgcggcaa agcagatggg cggcgtgatt
     9481 ccggcccccg gcaccggaag cggacatgcg gccgatgccg aggtcatggc aatggcggcg
     9541 gaactgccgg cccgtgttcg tggtcatgtc gagtcgcttc gtgtcgaaac cgcaatcgaa
     9601 accgtcatga attttgtgcg gcgcctgaac cggtatgtcg ctgaaacgga accgttcaag
     9661 gtcatcaaga ccgatccggc cgcggccggc ggcgcggttt atatcgcgct ggaaggcctg
     9721 cggattgcat tgaacctttt gtggccggtg atgccgaaga aggtcgaaaa cctgcttggc
     9781 gcgctggg
//
LOCUS       DAOPPA010000059         9620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8020_length_9620_cov_3.877574, whole genome shotgun sequence.
ACCESSION   DAOPPA010000059 DAOPPA010000000
VERSION     DAOPPA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_9620_cov_3.877574"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..583
                     /gene="purE"
                     /locus_tag="PKH54_03035"
     CDS             113..583
                     /gene="purE"
                     /locus_tag="PKH54_03035"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOC98891.1"
                     /translation="MEVVKVGVLVGSPSDKEIAEAIVKTLESLGIRGEIKVASAHRTP
                     DLVSAYCASAEGRGIQVLVACAGMAAHLAGAVAAQSLLPVIGVPVASGKLQGFDALLS
                     TVQMPPGIPVATVAIDGAKNAAVLAARIIALREPAVRQALEAALLADRRKYTDQ"
     gene            complement(616..1026)
                     /locus_tag="PKH54_03040"
     CDS             complement(616..1026)
                     /locus_tag="PKH54_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98892.1"
                     /translation="MKIPRIETDEIEAALTKMFGAFIAGETFSVSGMFEPGFLHIDLK
                     LHRRDDTFCYAMQFRCALAENRLDQGAALDVTVDFAGWYLEQYFDSGRELLLPLDFQP
                     YNFGDHIVYARGDIDNPKLTKMADEILNRGVICD"
     gene            complement(1007..1756)
                     /gene="rnc"
                     /locus_tag="PKH54_03045"
     CDS             complement(1007..1756)
                     /gene="rnc"
                     /locus_tag="PKH54_03045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOC98893.1"
                     /translation="MKESSNRVDISELENNVGHKFNDVDLILKALTHSSFCNETEDDV
                     SDNERLEFLGDAIISTVIAEAAFRLFPDAREGELTRLKSVVVAQPSLAATATHLEIGK
                     FMRLGHGAAVADQAATHPSVLCDAFEAVIGAIFLDGGFEKARQVVLGLLAGELEKARR
                     EGRHIDPKTELQMLTYGLAHKPPAYSVVETEGPAHAPVFTVQVTLPDGTVYRASGRSR
                     KQAEQAAAGMALENVRESLRVVEVGHEDSKD"
     gene            complement(1753..3132)
                     /locus_tag="PKH54_03050"
     CDS             complement(1753..3132)
                     /locus_tag="PKH54_03050"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOC98894.1"
                     /translation="MPAKDSREVFPGGGGKVRLAFRTTGCKVNQFDTEAILEAVSDMP
                     VIICDPDDRPDLVVVNACTVTMNADRDGRAAAFRALRTGAGVILAGCMATRLQDQGTA
                     STMPPEISIIGGTADRSALLSGIRERVRKILASGLPEPGRDAAGVATPPDDVHPAITA
                     AATKKGVRARPLVKIQDGCDCRCTYCIVPLVRGASTSRSIDDILNQVRRAADAGAAEI
                     VLTGVDLAAWGRNSDGRYGRLDELLVRLTAAGTGSRFRLSSLEPHGLSRELLDSMAAS
                     PDICPHLHIPMQSGSDAVLARMNRPYSAGRFAELVMEAAARVPGLVLGMDVIAGFPGE
                     TDRDFLLTLEMVQALPTTYLHVFPYSPRPGTPAATMDDQVPDGIRKERARALRQFGTR
                     ARSAHAGALTGSTVEVVDIRVGPEGVEALAADYTRVFVTNRHRILDGRTSVRIVSSRG
                     AVAIGRQEE"
     gene            complement(3142..3615)
                     /gene="smpB"
                     /locus_tag="PKH54_03055"
     CDS             complement(3142..3615)
                     /gene="smpB"
                     /locus_tag="PKH54_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOC98895.1"
                     /translation="MAKGNRQEKDGRKLVAQNRRARHDYELFEKFEAGLVLLGSEVKS
                     LRDGNAQLVDGYVEIRNMEAWLVGINIAQYSHSSFLNHDPRRDRKLLLNARELKRLDQ
                     KTREKGFTIVPLEIYFRDGLAKVEIALAKGRKAYDKRDKIEAKDLARASRGRDSD"
     gene            3766..5661
                     /locus_tag="PKH54_03060"
     CDS             3766..5661
                     /locus_tag="PKH54_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC98896.1"
                     /translation="MKIGIVAAIAVLLAFVPAASVGLPAPGVGLSYIDSRGCSISLQR
                     LPSESDRVAVFDGGDPARPILLRDGLLVAFDASVERVPSSVLARAGLTPVRPIDRAGT
                     TWLCRAAGPDQVLEASRALAVIEGVRWAVPDFSVPIDLHEAPGDPFWSAQWPVNPSGS
                     FHDVNVIEAWSRTTGDPRVIVAIIDTGVDTFHPDLGADRFVAGDNYIEARQGPWPGDR
                     AAAAHGTHCAGIIGALHNDIGIAGLCPGCSLAGYRFLTGLPGDEHVAELSNAAIALRQ
                     AADDGAWVISNSWGIYNVNKPKVDLVPIFEAARYAAENGGPDGQGALVVFSSGNQMGY
                     DESTDTTFALNIAPDDLAALPGVLAVGAINPEGERQEYSQWGPELDLVAPGGAYAALE
                     PQIVTLDTSGSTSGRGNGANKGDGQHYVSAGIGDVKKSGMPESDSSGLVTQYFNGTSS
                     SAPFVSGAAALVWSADLLDGVRDLSASDVRRILLDTAAKVGPLPYPAGRNDEYGRGLV
                     DVSAAVQAALDGGLAPADEVEQPMADAKVAEPSEPDPEQIGEMVRDAADTAAQMDQAS
                     DLAVDSANDVLNDSDASVSGSRGCSSASESGRNSILPILFLSMFALVVIRLTRRLPQC
                     TKSVSKA"
     gene            5776..8094
                     /locus_tag="PKH54_03065"
     CDS             5776..8094
                     /locus_tag="PKH54_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOC98897.1"
                     /translation="MSITQCAAELCAKARSEGRDTLLEPELYSLLAEGGVPVPANFMV
                     TPDAMAVAAIAGAPRLPGSRVVVKVVSPAITHKTEMGGVRFVENTPEAISEACTGIIQ
                     AVTERGGPELAATVRGLMVSEMVPVGHDISSQLFAGMRFSPDVGPVLAFGFGGLEAEE
                     LAARFRDGQGVVLMSPLVMTPEQMLCKFRKSFVYRKLAGLTRGGARQVSDEELMKVFE
                     FFIDLAVNFSNQPGTGWLVKDFEVNPFFVSEGRLVAVDAFMRFCPELVVERVCDLEKT
                     HTLLHPKTVAIMGASSKGINVGRIILANLLKKGFPSRLIRVVRPDCEPVDGVEAVATI
                     SDLPWVADMVVVAVGAKQVPAIIKESLECGKAASMVLIPGGMGETEEGKATDREIRSL
                     IREARAAGKFVPVFVGPNCLGVSSVPGNYNTLFVPETKLPPQDASQSRDNAEVALICQ
                     SGGFLITRMMNMPWVEPRYAVSTGNQMDLALVDFVESILKDDQVEVFGLYIEGFKPLD
                     GLRLARLIRKGRAAGKDFVVYKAGRTCAGQTATSSHTASISGDYDSCYEVLADAGALM
                     TESLEEYSELMQMAIAFKRKKINGTRLACLSNAGYETVGMADAICEKSQFTLGDYDEK
                     TAATIQNVLTMGRLAGLVDIHNPLDITPMAPDLVYAGCLQAQISCPNIDACIVGILPL
                     TPAMKTLPPGVDPAGVDTILAPDALPALFSKIAHYSAKPVITTVDCGRLYDPLCDELR
                     KNGVPCFKSVDSAMKSLQRYMLYRLGLSENVC"
     gene            complement(8135..>9620)
                     /locus_tag="PKH54_03070"
     CDS             complement(8135..>9620)
                     /locus_tag="PKH54_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOC98898.1"
                     /translation="GFKDLTRFLSPLGIEPSAPSFDPVLAAYVLDAGSGQFGLLDVLE
                     RTIHASLPNRADVSPEAISAILLAQRSGNAILRENVAAANLGYLLKDVEIPLAGILGR
                     MEQKGVLVDRQALSDLSHSLGEDLRAVERRIIDAAGVAFNPMSPKQLADVLFVKLGLP
                     AGKKKSSGYSTDVKVLEELAPLHPVPELVLEYRSLSKLKGTYADALYNLVDPADGRIH
                     STFNQTVTATGRLSSSDPNLQNIPIRTEAGRRIRHAFRAPEGRQFVSADYSQIELRVL
                     AHFAADPFLAEAFANRRDIHSETARRIYRLPAGGEGSQVTPEMRRVAKIVNFGLLYGM
                     GPFRLAGDLKIPQKDARQIIDDYFNAFPGIRDFLSKTVEQTRQTGFSTTLFGRRRGIA
                     DINSPNHQLRTAAERMALNMPVQGTAADIIKIAMVNLDRRLKRDGVRAEIVLQVHDEL
                     VLEVDNDEVQRAREILISEMENAAALAVPLVAEPGHGTWWDELH"
BASE COUNT         1759 a         2903 c         2916 g         2042 t
ORIGIN      
        1 ccgacttcct gcagaagtcg gtcgtcaagg ctttcatcgc ctatgacaag gcgttggcat
       61 cattcaggaa ggcgggcggt catccctgac ccgcggcaac cggaggggtt taatggaagt
      121 cgtcaaggtt ggagttcttg ttggcagccc aagtgacaaa gaaattgccg aagcgattgt
      181 caaaaccctg gaatcgcttg gaatcagagg cgaaatcaag gtggcatccg cccaccgcac
      241 gcctgacctt gtaagtgctt actgcgcctc ggcggaaggc cgtggcatcc aggttcttgt
      301 cgcctgcgcc ggaatggcgg cgcatcttgc cggggccgtc gcggcccagt cgcttctgcc
      361 ggtaatcggc gtgcccgtcg cttccgggaa gctccaggga tttgacgccc tcctttcgac
      421 cgttcagatg ccgccgggaa tccccgtggc caccgttgcc atcgacggtg cgaagaatgc
      481 cgccgttctc gccgcgcgga tcatagccct ccgggagcct gcggtcaggc aggcgcttga
      541 agcggctctt cttgccgatc gcaggaagta caccgatcag tgatttgatt cggtcgattt
      601 tcccgtctgg ccgcttcagt cgcagatcac gcctcggttc aggatttcat cggccatctt
      661 tgtaagcttc gggttatcga tatcgccccg ggcatagacg atgtgatccc cgaagttgta
      721 gggctggaag tcaagcggca gaagcagttc gcgaccgctg tcgaagtact gttccaggta
      781 ccagccggcg aaatctactg tgacatccag ggccgcaccc tggtcgaggc gattctcggc
      841 aagtgcgcac ctgaattgca tggcgtagca gaatgtgtcg tcccgcctgt gaagtttcag
      901 gtcgatgtgc aggaagccgg gctcgaacat gccggacacc gaaaatgtct ccccggcaat
      961 gaaggccccg aacatctttg tgagggcggc ctcgatttcg tcagtttcaa tccttggaat
     1021 cttcatgtcc gacctccacc acgcgaagag attcgcgaac attttcaagc gccatgccgg
     1081 cggccgcctg ttccgcctgc ttcctgctgc ggccgcttgc cctgtatacg gttccgtccg
     1141 gaagcgtcac ttgaaccgtg aatacgggcg cgtgggcagg gccctcggtt tcgaccacgg
     1201 agtatgccgg aggcttgtgg gccagtccat aagtaagcat ctgaagttcg gtcttggggt
     1261 cgatatgccg cccttcgcgc cttgccttct ccaactcgcc ggccagaagg ccaagaacga
     1321 cctgcctggc cttctcgaag ccgccgtcaa ggaatatcgc gccgattacc gcctcgaagg
     1381 catcgcacag aacggacgga tgcgtggcgg cctgatccgc gacggcggca ccgtgaccaa
     1441 ggcgcatgaa cttgccgatc tccaggtggg tcgcggttgc ggcaagtgac ggctgcgcga
     1501 cgaccaccga tttcaggcgt gtaagttcac cctccctggc atcggggaaa agcctgaacg
     1561 ccgcctcggc gatgaccgtt gaaatgattg cgtcgcccag aaattcgagc ctttcgttat
     1621 cggaaacgtc gtcttcggtc tcgttgcaga acgacgaatg cgtcagggcc ttcaagatca
     1681 gatcgacgtc gttgaacttg tgtccgacat tgttttccag ttcggaaata tccactctgt
     1741 tcgatgattc cttcattctt cctgccgtcc aatggcaacc gcgccgcgac tggaaacgat
     1801 tcgaaccgaa gtccttccat cgagaatgcg atgcctgttc gtgacgaaga cccttgtgta
     1861 gtctgccgca agtgcttcga cgccttcggg gccgaccctt atatcaacga cctccaccgt
     1921 gcttcccgtc agtgccccgg catgggccga ccgcgcgcgc gttccgaact ggcgcagggc
     1981 gcgggcccgt tccttccgga ttccgtcagg tacctgatcg tccatggtgg ccgccggcgt
     2041 cccgggacgt ggcgagtacg ggaatacatg aagataggtc gttggcagtg cctgaaccat
     2101 ctcaagtgtc agcaggaagt ccctgtccgt ctcgccgggg aagccggcaa tcacgtccat
     2161 cccaagaacc agtcccggaa cgcgtgccgc cgcttccatg accagctcgg cgaaccggcc
     2221 tgccgagtac gggcggttca tcctggcaag aaccgcgtcc gaacccgact gcatcggtat
     2281 gtgcaggtgc ggacagatgt cggggctggc cgccatcgaa tcaagcagtt cgcggctgag
     2341 gccgtggggc tcaagcgatg agagcctgaa gcgcgacccc gttcccgcgg ccgtcagccg
     2401 aacgagcagt tcatccagtc gcccgtacct gccgtcggaa ttccttcccc aggcagccag
     2461 atccacaccg gtcaggacga tttccgcggc accggcgtcc gcagccctcc ggacttgatt
     2521 caggatgtcg tcaatcgaac ggcttgtcga cgctcccctg accaggggaa ctatgcagta
     2581 agtgcagcgg cagtcgcagc cgtcctggat cttgaccagc ggcctggccc tgacgccctt
     2641 tttggttgcg gcggcagtga ttgcaggatg aacgtcgtcg ggcggcgtcg ccacgccggc
     2701 cgcatccctt cccggctctg gcagtcccga ggccagaatc ttccggacgc gttccctgat
     2761 gccggaaaga agggccgacc tgtcggccgt gccgccgata atggagattt ccggcggcat
     2821 cgttgaagcg gttccctggt cttgaagcct tgtcgccatg catcccgcaa ggattacccc
     2881 ggctccggtc cgcaatgccc gaaaggccgc ggcccttccg tccctgtccg cattcattgt
     2941 gacggtgcac gcatttacaa ccaccagatc cggtcggtcg tcaggatcgc agatgattac
     3001 cggcatgtcg gagacggctt caagaatagc ttccgtgtcg aattgattga ccttgcaacc
     3061 ggtggttctg aaggcaagcc gaaccttgcc tccgcccccc gggaaaacct ctcttgaatc
     3121 tttcgcaggc aaaaaggccc cctagtcgct gtcgcggccc cggcttgccc tggccaggtc
     3181 cttcgcctcg atcttgtccc gcttgtcgta cgccttcctg cctttggcga gcgcgatctc
     3241 gacctttgcc aggccgtccc ggaagtaaat ctcaagcggg acaatggtga atcccttttc
     3301 cctcgtcttt tgatccaggc gcttcagttc ccttgcgttc aggagcagct tgcggtcacg
     3361 cctggggtca tgattcagaa atgacgagtg cgaatactgg gctatattga tgccgacaag
     3421 ccatgcctcc atgttgcgga tctccacgta tccgtcaaca agctgggcat tgccatcccg
     3481 aagggacttg acttcgcttc ccagcagaac caggccggcc tcgaacttct caaacagttc
     3541 gtagtcgtgc ctggctcgcc tgttctgggc gaccagcttg cggccgtcct tttcctgtcg
     3601 gttacccttt gccatcccgg tctgtccgtt cgcgccattt ccggcgcgac agtataaccg
     3661 atttgcgcga tccggtcagc caggccgtcc ggaacagctt cgatgttgcc acccgcgtgg
     3721 tttgccacga taatgggagt cctggatgag acccttggaa atactgtgaa aatcggaatt
     3781 gtggcagcga ttgcagtttt attggccttc gtcccggcag cttccgtcgg acttccggcc
     3841 ccgggcgtcg ggctctccta cattgactca aggggctgtt cgatatcact ccagcgcctt
     3901 ccctcggaga gcgacagggt cgccgtgttc gacggagggg atcctgccag gccaatcctg
     3961 ctgcgggacg ggctgctcgt ggcgtttgat gcatccgttg aaagagtacc ttcgtcggta
     4021 ttggccaggg cggggcttac cccggtcagg cccattgatc gggccggaac cacctggttg
     4081 tgccgagccg ccgggccgga tcaggtgctg gaggcttccc gggcgctggc cgtcatcgag
     4141 ggggtacgct gggctgttcc cgatttttct gtgccgattg atctgcacga ggctccaggg
     4201 gatccattct ggtcggcaca atggccggtc aacccgtccg ggagttttca tgacgtcaac
     4261 gtcatcgagg cctggtcaag aaccaccggg gatcctcgtg tcattgtcgc gataatcgac
     4321 accggcgttg atacgtttca tccggatctt ggtgccgaca ggttcgtcgc cggggacaac
     4381 tacatcgaag cccggcaggg gccctggccc ggggatcgag cggccgccgc ccatggaacc
     4441 cattgcgcgg gtatcatcgg cgcactccac aacgacattg gaattgcagg gctctgtccc
     4501 ggttgcagcc ttgccggata caggttcctg accggacttc cgggcgacga gcacgtggcc
     4561 gagctttcga acgcggccat tgcgctgagg caggccgccg acgacggtgc gtgggtcatc
     4621 agcaattcgt gggggatata caacgtcaac aagcccaagg tcgatctcgt gccgatcttc
     4681 gaggccgcca gatatgcggc ggaaaacggg ggaccggacg ggcagggcgc ccttgtggtc
     4741 ttttcgtccg gcaatcagat ggggtacgac gaatcaacag ataccacgtt cgccctgaac
     4801 atcgcgcctg atgatctggc ggcccttccg ggggtgctgg cggtcggcgc catcaatccc
     4861 gaaggtgaaa gacaggagta ttcccagtgg gggcctgaac tcgatctggt cgcgcctggc
     4921 ggtgcgtacg cggcgctgga gccgcagata gtgacgctgg acacgtcagg ctcgacatcc
     4981 gggcggggca atggcgccaa caagggtgat gggcagcatt acgtgtcggc cggcatcggc
     5041 gacgtcaaga agtcaggcat gccggaatcg gattcatcag gcctggtgac gcagtatttc
     5101 aacggcactt cttcctcggc tcctttcgtt tccggtgctg cggcgcttgt gtggtccgcc
     5161 gaccttctgg atggggtgcg ggatctttcc gcatccgatg tccggcgcat cctgctcgat
     5221 accgccgcaa aggttggtcc cctgccatat cccgcaggcc gcaatgatga atacggccgg
     5281 ggcctggtcg atgtgtccgc cgcggtgcag gccgctcttg acggggggct ggcgccggcg
     5341 gacgaagtcg agcagccgat ggccgatgca aaggtcgccg aaccttctga acccgatccc
     5401 gagcagattg gggaaatggt gcgcgacgct gccgacacag ccgctcagat ggatcaggcg
     5461 tcggatctcg cggtcgattc ggcgaatgat gttctgaacg attcggacgc ttccgtttcc
     5521 ggttccagag gttgttcatc ggcctctgaa tctggtcgga attcaatcct gcccatcctt
     5581 tttctgtcca tgtttgcgct ggttgtaatc cgtctgacgc gccgcctgcc gcaatgcaca
     5641 aaaagcgtgt caaaagcctg aaacagcggt tgaatttatg acacacggcg ttattatgcc
     5701 gtgaaactgc gggcaaactg ccgaaagtgg cagcccggtg cagtcgattg ctgaaggtta
     5761 accagggaga ctccgatgtc gattacgcag tgcgccgccg aattgtgtgc gaaagcccgt
     5821 tccgagggaa gggacaccct tcttgaacca gagctttatt ccctccttgc cgagggcggt
     5881 gtcccggttc cggccaactt catggtcact ccggatgcaa tggcggttgc cgccatcgcc
     5941 ggggctccca ggcttcccgg ttccagggtt gtcgtaaagg ttgtttctcc cgccataacc
     6001 cataagaccg agatgggcgg cgtcaggttc gtcgaaaaca cccccgaggc catatcagag
     6061 gcctgcaccg gaatcattca ggccgtgacc gagcgcggcg ggccggaact cgccgccacc
     6121 gtgcgcggac tgatggtttc cgagatggtt ccggtcggtc acgacatttc atcccagctt
     6181 tttgcaggga tgcggttctc accggacgtc gggcctgttc tcgcattcgg attcggcggc
     6241 ctggaggcag aggaactcgc cgcgcgtttt cgcgacggcc agggcgtcgt cctgatgtcc
     6301 ccgcttgtca tgaccccgga acagatgctt tgcaagttcc ggaagtcctt tgtttaccgg
     6361 aagctggccg ggctgacccg cgggggtgcc aggcaggtgt cggacgaaga attgatgaag
     6421 gtctttgagt ttttcatcga cctggccgtc aatttttcga atcagccggg gacgggttgg
     6481 ctggtcaagg acttcgaggt caatccgttc ttcgtgtcgg aaggccgcct tgtcgcggtt
     6541 gacgccttca tgcgcttctg tccggaactg gtcgtcgagc gcgtgtgcga tctggaaaag
     6601 acgcacacat tgcttcaccc gaagaccgtc gccatcatgg gagcctcttc caagggcatc
     6661 aacgtaggtc gaatcatcct tgccaatctt ctgaagaagg gctttccttc ccgcctgatt
     6721 cgcgtggtca ggccggactg cgagccggtg gacggtgttg aggctgtcgc cacgatttcc
     6781 gatcttccct gggttgccga catggtagtc gtcgcggtcg gcgcgaagca ggttccggcg
     6841 atcatcaagg agtcgctgga gtgtgggaag gccgcttcga tggttcttat tcctggtgga
     6901 atgggcgaga ccgaggaagg gaaggcgacc gaccgtgaga ttcgctcgct gattcgcgag
     6961 gcccgtgccg caggaaagtt cgttccggtt ttcgttggtc caaactgcct gggcgtcagt
     7021 tccgtgcccg gaaactacaa caccctgttt gttcccgaaa ccaagctgcc tccccaggat
     7081 gcaagccaga gtcgcgacaa cgccgaagtg gcgctgatct gccagtccgg cggcttcctg
     7141 atcacgcgca tgatgaacat gccctgggtc gagccgcgtt acgccgtctc tacgggcaac
     7201 cagatggatc ttgctctggt cgatttcgtc gaatcaatcc tcaaggatga ccaggtcgag
     7261 gtttttggcc tttacatcga gggtttcaag ccgctggacg gtcttcgtct tgccaggctc
     7321 atccggaagg gacgcgccgc gggtaaggat ttcgtcgtat acaaggcggg ccgcacatgt
     7381 gccggccaga ccgccacatc gtcgcatacc gcttcgatat ccggcgacta cgactcgtgc
     7441 tatgaggttc tggccgacgc gggcgccctg atgaccgagt cgctcgagga atacagcgaa
     7501 ctgatgcaga tggcgattgc cttcaagcgc aagaagataa acggtacaag gctggcctgc
     7561 ctttccaacg ccggatacga gactgttggg atggccgacg cgatttgcga gaagtcgcag
     7621 ttcacgcttg gcgactatga cgaaaagacg gcggccacga tacagaacgt gcttacaatg
     7681 ggtcgtcttg cggggctggt cgatattcac aacccgcttg acatcacccc catggctcct
     7741 gatctggtct atgcaggttg cctgcaggcc cagatttcat gtccgaacat cgatgcgtgc
     7801 atcgtcggga ttctgcctct gactccggcc atgaagacgt tgcccccggg agtcgatcct
     7861 gccggcgttg ataccatcct ggccccggac gcacttcctg ccctgttttc aaagattgcg
     7921 cactactcgg caaagccggt catcaccacg gtcgattgcg gacgccttta cgacccgctc
     7981 tgcgatgaac tgcgcaagaa cggcgtgccg tgcttcaaga gcgtcgattc cgcgatgaaa
     8041 tcgctgcagc gctacatgct ttacagattg ggcctttcgg aaaacgtctg ctgacatcgg
     8101 tgattgccgg ggttgctccg gcgcggttcc tttttcagtg aagctcgtcc caccaggttc
     8161 catgaccagg ctcggccaca agcggcacgg ccagcgcggc cgcattctcc atctcggaaa
     8221 tcaggatctc ccttgcgcgc tggacttcgt cattatcgac ttccagaacc agttcgtcat
     8281 gcacctgcag gacgatttcg gccctgaccc cgtcgcgttt aaggcgcctg tccagattga
     8341 ccatcgcaat cttgatgatg tcggccgcgg taccctggac cggcatgttc agggccatgc
     8401 gctcggccgc tgttcgaagc tggtggtttg gtgaatttat atccgcgatc ccacgccgcc
     8461 gcccgaaaag ggtcgtcgag aagccggtct ggcgtgtctg ctcgaccgtc ttcgacagga
     8521 agtccctgat tcccgggaag gcgttgaaat agtcgtcgat tatctgcctg gcatctttct
     8581 gtggaatttt caggtcgccg gccaggcgga acggtcccat cccgtaaagc agtccaaaat
     8641 tgacgatttt cgcgactcgt cgcatctcgg gagtcacctg tgaaccttcg cccccggccg
     8701 gcagccggta aatccggcgc gccgtttccg agtggatgtc ccggcggttg gcgaatgcct
     8761 cggcaaggaa cggatctgcg gcgaagtggg caagaacccg aagctcgatc tgcgaatagt
     8821 cggccgaaac gaactggcgg ccctccggcg ccctgaacgc atgcctgatg cgcctgccgg
     8881 cctcggttcg tatcgggatg ttctgcaggt tggggtctga ggatgaaagt cgtcccgttg
     8941 cggtcacggt ctggttgaag gtgctgtgaa tccttccgtc ggcgggatcg accaggttgt
     9001 aaagcgcgtc cgcgtaggtg cccttcagct tggaaagcga acggtattca aggacaagtt
     9061 ccggaaccgg gtgcagcgga gcaagttcct caagaacctt cacgtcggtc gagtaccccg
     9121 aggatttctt ttttccggct ggcaggccga gcttcacgaa caggacatcc gcaagctgct
     9181 ttggcgacat cgggttgaaa gcgactccgg ccgcatcgat tattctgcgt tcaaccgcgc
     9241 gaaggtcttc cccaagcgaa tgcgacaggt ccgaaagcgc ctggcggtca accaggaccc
     9301 ccttctgctc catgcggccg agaatgccgg ccagggggat ttccacgtcc ttcagcagat
     9361 atcccagatt ggccgctgcg acgttttccc gaaggatggc gttgccggaa cgctgtgcca
     9421 gcaggatcgc cgaaatcgct tccggcgaaa catcagccct gttcggcagt gatgcatgga
     9481 tcgtcctttc gagtacatcc agcagcccga actgaccgga tcccgcgtca aggacgtagg
     9541 ccgccagcac cgggtcgaag gacggcgcgg agggttcgat tccaagcggc gaaagaaagc
     9601 gcgtcaagtc cttgaacccg
//
LOCUS       DAOPPA010000060         9539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8099_length_9539_cov_3.791755, whole genome shotgun sequence.
ACCESSION   DAOPPA010000060 DAOPPA010000000
VERSION     DAOPPA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_9539_cov_3.791755"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKH54_03075"
     CDS             <1..1059
                     /locus_tag="PKH54_03075"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOC98899.1"
                     /translation="PKAQTGDYGLTLTERAPYGVICSITPSTNPTETIINNAIGMIAG
                     GNAVVINPHPNAKKTTVDTVRMLNQAVMAAGGPDNLICVIPEPTIESADRLMRHSGVR
                     LVVVTGGPGLVKAAMGTGKKCIAAGPGNPPALVDETAILPNAARDIVKGAGMDNNIIC
                     IVEKEIIAVEAIADRLKQELVRAGAYYIRDNVVPKLVDLVLKGHEPNRAFVGRNASVI
                     LKQVGIDIPDDVRIAFCEVDEEHPLVQAEQLMPIIPMFRVRNVDEGIKAALRVEHGFR
                     HTAVMHSLNIENLHRYARAADCSIFVKNAPSLAGTGFMGEGHTSWTIASPTGEGMTTA
                     INFTRERRCVLKDYFRIV"
     gene            1097..1702
                     /locus_tag="PKH54_03080"
     CDS             1097..1702
                     /locus_tag="PKH54_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC98900.1"
                     /translation="MSLTPARFFPLPDLQVVPEAFAGIEVHGIARGFVCIDALVKKAR
                     SRICLAATVTPGKFLILIDGSVAEVEESLLEAERLAGDKLIDRFFLPFAHEQLERGVF
                     GPMADPGAEALGVVECSTSCAGIRSADAALKAAMVKLNVIHLSAGIGGKCYFAFSGDL
                     FDVEASIQAAAESIGQDRLLGTEVIPNPHPEFIAALGISPV"
     gene            1805..2815
                     /locus_tag="PKH54_03085"
     CDS             1805..2815
                     /locus_tag="PKH54_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98901.1"
                     /translation="MYHQKTLDPTVDVTILRDTPACDSPVDFMLRFSRMCSHVFHLNL
                     GNVCNMSCRYCCLDHSHQYYATPESVADTIRKAARWNFRKCALVGGEPTIRRDLLHTM
                     QLLTASGINEITLTTNGLMLSYPKVVASLVNAGMRTVHLSIDDFDPETTVWLSRNPRC
                     GELVFKALMNLMGTPGVETFLYGVVTRANVAHMNNYVDSVAALKPISHKPTVIFSGMK
                     PAGSTSEDMDRIFLPLSESAAAIRAAIDRGREVGVGVGFKNIPNCLMPGYEGYNLDAY
                     LIESEFNLITGAYGGSVGSDRQKNGKTCGRCRWNEPCTGVFSEYTRKMGWDEFESGGV
                     RT"
     gene            2812..3765
                     /locus_tag="PKH54_03090"
     CDS             2812..3765
                     /locus_tag="PKH54_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98902.1"
                     /translation="MKFHTIIVGAGGFLGHRLCLAAASASTASGRPVDGIAGVSRRRP
                     IRQTGGVTRITGDRQRPDLAKMLLQSGATTWIDTAISSADDAVQLVSACRTVADSGIR
                     LPSFVITSTIGEHARILAEGGTIRSDSPLMADDEHARGKLEAFLVLADQPWLDVTWVI
                     LPMMWGPGDHDPDGRRGRTRDVIRAIMTGRPVRFAGNGDNAMPDGFVDTVAHAIVHIS
                     SSPSGPGIRRIPVAGPDPVTPAGFVAEVRSELGLEPEVPEDSGKDPRDGPHPFPGSAV
                     AMDCHELYESGFRAPFDWRTGVRLTVQAELVESGKAWGCQR"
     gene            3762..4730
                     /locus_tag="PKH54_03095"
     CDS             3762..4730
                     /locus_tag="PKH54_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98903.1"
                     /translation="MKTFREVARRVGPWLVTAGLIAWVAATTQFDGLRSAVALADFGT
                     FSILAVAFAAAHLLLDSAGLHALLQRTGIGIPYPRLLAIRASSYLLGFINYNLAFVLI
                     AAAAARGRSVRWQSLASPFIALNLIDLFVVSAILIAGLASGSGDHLKAGVPTLLAVGA
                     ILGLLGLPAVMAAAAHARAGRRKDAPSGLLHTFRASGPAGVAAVLGLRVILLAFDMSG
                     DFLMLGTFNLDIPVGVFMQLFAVDSFACVLPITVAGIGPTMVLMRAFFEPYVTQAGGS
                     TAAIDAFSASATASLAVIRTVTALCCMPFALKAGLGRTIKEIRGDQ"
     gene            complement(4758..6797)
                     /locus_tag="PKH54_03100"
     CDS             complement(4758..6797)
                     /locus_tag="PKH54_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC98904.1"
                     /translation="MSVSIASVIAMSLMLVVALAFFSVSMLKRIRVMLAGKPEVRWDR
                     PARRFAEMLKVAFAQSRMFKEPGPGIMHAFIFWGFLVLLFRSASLIGRAYAPEWSSWT
                     LFWFCPTLDGWYALLKDVTELVVLAMIAWAFFRRLVVRPKRLENSFSAFLILGFISYL
                     MVSDFIYDASRFALLSAGGVIPDAMASKIAHEREWSPIGAMVAGWLDGLSPGTLKVLI
                     QVFFWSHVGVLLVFLNELPLAKHFHVILAIPNVFFMRLTHPGAIEPIRDIEEQETFGV
                     LRPTELTWKQMMDGFSCTECGRCTVNCPAVCTDKQLNPKRLICDMRDYVRENEKAILS
                     GGDLGEKAREDGRSLIDAIRDEVIWECTTCRSCEEQCPVTITHVDKIIGYRRYLTLME
                     ASMDPELATCQKNLENKSNPWGLPSGERGLWMVEELGVPLLSDKPDAEYLFFLGCSAA
                     YDDRNRKAARAFVKLLNAAGVSFAILGPEEGCCGDTARRLGNEYLFQAQASQNIEVFK
                     EYGVRKIVTMCPHGFNTIRNEYPQFGGAFEVIHHTSLLAALVAEGRLSFEGGKSDAVV
                     YHDSCYLGRYNGIYEDPRKALAGAGLSLVEADRSGKTAMCCGAGGGMMFRDEKEGSRI
                     NRLRVNQLVETGAKSIVSACPFCLVMCRDGINEEGIDQNVSAFDVSEVLAERLKA"
     gene            complement(6840..>7535)
                     /locus_tag="PKH54_03105"
     CDS             complement(6840..>7535)
                     /locus_tag="PKH54_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98905.1"
                     /translation="VSERQFHAFEGEDLGPEAIIGGEGRLVLDKIPEYFQRFERLILP
                     SRPDTVQNFRKHLDGRPVVVEVGSGRGRLLCEVALSRPDAVCLGLETGLGLCGDALKR
                     AEKKGASNLFMAWGDARRTIPMLVDPGSADAAYLLFPDPWWKKKHASRRHGALTGITI
                     ANALRSGGILHLKSDVEPYLHEIVAAFLSTGMYDRLTDDEAVIAAMPLTDREVRLTAA
                     GTPVFAAVLLRRA"
     assembly_gap    7536..7545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7761..>9539
                     /locus_tag="PKH54_03110"
     CDS             7761..>9539
                     /locus_tag="PKH54_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98906.1"
                     /translation="MRPVITVFSALVAIHMAGCGGGSQPGEDVNIPVDITLDQGGLDA
                     FDAAYDEGRDGMTQADGIDALSQDTEDVGDPCAMTPMGFGCICDENPDCESGYCIKMP
                     HENYLRCTRLCSSKCPEGWECSQVLLGGGDSAYACMPDYNQLCQSECSENSQCTAAGA
                     LCIQYGNKKYCGQSCNQDSDCESMTRIKDCDNTGECSFKNETTIEECLEDEVTCEVIE
                     LDFKCVEATSIDGTTISRQCVPTSDHCECASDTDYDNDPNHCGDCETRCFYPNATASC
                     SGGKCKLDACLRGYVNLNGVPDDGCEYECLFQSTVDHPDPEYIDADCDGIDGDVETGV
                     FVMNGGDDDNFIGSMAFPFATIGAAMAFAAEQSPIPEVYVSKGIYQEQVNLVNGVSVY
                     GGYDGDSGWKRNLVTNQSIISWNGQETLAVRAVVARNITEPTTFDGFHVKSSQSSQLS
                     GSSYGMHVMGCDEDLVISNNYLEADSGTQGGNGNFGTNGQAGTNGTKGSDAFVYDGCG
                     LCICTKFDFNTNMKPGPGGGSPCSMDGGKGGAGGKLGDGGLAGTAATGSGGAGGAGAP
                     DEQTDGSVGKPGPDGNDGTDGGSGVAS"
BASE COUNT         2001 a         2922 c         2824 g         1782 t
ORIGIN      
        1 ccgaaggctc agacaggcga ttacggactc accctgaccg agcgcgcccc atacggggtc
       61 atctgctcga tcacgccgag cactaatccg actgaaacga tcatcaacaa cgcaatcgga
      121 atgatcgccg gcggcaacgc tgtcgtgatc aacccccacc cgaacgcgaa gaagacgacg
      181 gtcgataccg tcaggatgtt gaaccaggcc gtaatggcgg ccggtggacc ggacaacctt
      241 atctgcgtca ttccggagcc gaccatcgaa tcggccgaca ggctgatgcg ccacagcggc
      301 gtgcgccttg ttgtcgtcac cggtggcccg ggcctcgtca aggccgcgat gggaaccggc
      361 aagaagtgca tcgcggccgg cccaggcaat cctccggcgc tggttgacga aacggccata
      421 cttcctaatg ccgcaaggga catcgtcaag ggtgcgggga tggacaacaa catcatctgc
      481 atcgtcgaaa aggaaatcat cgcggtcgag gcgattgccg acaggctgaa gcaggaactg
      541 gtcagggccg gcgcctacta catcagggac aacgtcgtgc cgaaacttgt cgatctcgta
      601 ttgaagggtc acgaaccgaa tcgtgcgttc gtcggaagga acgcttcggt catcctcaag
      661 caggtgggaa tcgacattcc cgacgatgtc aggattgcat tctgcgaagt cgacgaggaa
      721 cacccgctgg ttcaggccga acagttgatg ccgatcatcc cgatgttccg ggttcgaaac
      781 gtcgatgagg gaatcaaggc ggcacttcgg gttgaacacg gattccggca taccgccgtg
      841 atgcattcgc tgaacatcga aaacctgcac cggtacgcgc gcgcggccga ctgctcgatc
      901 ttcgtgaaaa acgcgccgtc gctggccggc accggtttca tgggtgaggg tcatacgtcg
      961 tggacgattg ccagcccgac cggggaaggc atgaccacgg caatcaactt cacccgcgag
     1021 cgcagatgcg tgctcaagga ctatttcagg atcgtctgac gccccctttt cgggcgagat
     1081 cctttgcgga ggccaattga gccttacacc agccagattt tttccactgc ccgaccttca
     1141 ggtcgtcccc gaggcattcg cggggattga agtacacggg atcgcacggg gcttcgtctg
     1201 catcgacgcg cttgtcaaga aggcccgctc gcgcatatgc ctggccgcga ccgtgactcc
     1261 gggaaaattc ctgatactta tcgacggaag cgttgccgag gtcgaggaat cgctgctgga
     1321 agccgaaagg cttgcaggcg acaagctgat tgacaggttt ttccttccat ttgcacacga
     1381 gcagcttgag cgcggggttt tcgggcccat ggccgacccg ggggcagaag cactgggtgt
     1441 cgtggaatgc tcgacctcgt gcgccggcat cagatcggcc gacgcggcat tgaaggcggc
     1501 catggtcaag ttaaacgtca ttcacctttc ggcgggcatc ggcggcaagt gctacttcgc
     1561 tttttccggc gacctcttcg acgtggaggc atcgatccag gccgcggccg aaagcatcgg
     1621 ccaggacagg ctgctcggca ccgaagtcat accgaatcca caccccgaat tcatcgccgc
     1681 actcggtatc tcacctgtct gacgacaaag agccttccca ttgccaatgc cccatcccaa
     1741 ctttttatgg ccacggcgac ggcggcgact ataatcgttt tcgggggtgc ggccggggga
     1801 accgatgtat caccagaaga ctcttgatcc caccgtcgat gtaaccattc tgcgcgacac
     1861 tccggcatgt gattccccgg tggatttcat gctgcgcttc agcaggatgt gcagtcatgt
     1921 gttccacctg aacctgggca acgtctgcaa catgtcgtgc cggtactgct gcctggatca
     1981 cagccaccag tactatgcca cgccggaatc ggtggccgac accatcagga aggcggcccg
     2041 ctggaatttc cgcaagtgcg cgctggtcgg gggcgagccg acgattcgca gggatctgct
     2101 gcatacgatg cagctcctta ctgcgtcggg catcaacgaa atcacgctta cgacaaacgg
     2161 attgatgctt tcctacccga aggtcgttgc cagccttgtg aatgccggca tgcggacggt
     2221 acacctgtcc atcgacgact tcgatccgga gactaccgtg tggctttcca ggaatccacg
     2281 ctgcggggaa cttgttttca aggctcttat gaacctgatg ggaactccag gcgtagagac
     2341 gttcctgtac ggcgtggtca caagggcaaa cgttgcccac atgaacaact acgtggattc
     2401 ggtggcggcc ctgaagccga tttcccacaa gcccaccgtg attttttccg gcatgaagcc
     2461 ggccggaagc acctcggagg atatggatcg catcttcctg cccctgtccg agtcggcggc
     2521 ggcgatacgg gccgcaatcg accgcggacg cgaggtcggg gtcggcgtcg ggttcaagaa
     2581 catcccgaac tgcctgatgc cgggatacga gggctataac ctggacgcgt acctgatcga
     2641 atcggaattc aacctgatta ccggggccta cggcgggtcc gtcggcagcg acaggcagaa
     2701 gaacggaaaa acgtgcggcc gctgccgctg gaacgaacct tgcaccggtg tattttccga
     2761 atacacccga aaaatgggct gggacgaatt cgagtcgggc ggggttcgga cgtgaaattc
     2821 cacaccatca tcgtcggggc cggaggattc ctgggacatc gcctgtgcct ggccgcggca
     2881 tccgcatcga ccgcttccgg gcgtccggtc gacgggattg cgggagtctc caggcggcgc
     2941 ccgatccgac agacaggcgg cgtcacccgg attaccggag accggcaacg acccgatctg
     3001 gcgaagatgc ttctgcagtc cggcgccacc acatggattg acacggcgat atcgagtgcc
     3061 gacgacgccg tccaactggt cagcgcgtgc cggacggtgg cggattccgg catcaggctc
     3121 ccatcattcg tcatcacgtc cacgatcggc gaacatgcac gaatcctggc tgaaggcggg
     3181 acgataagat ccgactcgcc gctgatggcc gacgacgaac acgccagggg aaagcttgaa
     3241 gccttcctgg tccttgcgga tcagccctgg cttgatgtca catgggtcat cctgccaatg
     3301 atgtgggggc cgggcgacca cgacccggac ggccggcgcg gaaggacaag ggatgtcatc
     3361 cgcgccatca tgaccggacg accggtacgg ttcgcgggca acggcgacaa cgccatgccc
     3421 gacggcttcg tcgatacggt tgcgcacgcg attgtccaca tctcatcaag cccttccggg
     3481 cccggcattc gccgcattcc ggtcgccggg ccggatccgg tcaccccggc gggttttgtg
     3541 gccgaagtcc gcagcgaact cgggcttgag cccgaagtcc ctgaagattc cggaaaagat
     3601 ccccgcgacg gcccgcaccc gtttcccgga agtgcagtcg caatggactg ccacgaactg
     3661 tatgagtccg gattccgggc accctttgac tggcggaccg gcgtcaggct taccgtgcag
     3721 gcggagcttg tcgaatcagg aaaggcatgg ggctgccagc gatgaaaaca ttcagggaag
     3781 tggcgcgcag ggttggtccg tggctcgtaa ccgcgggcct catcgcctgg gttgcggcaa
     3841 caactcaatt cgatggcttg cgctcggcgg tagccctggc ggatttcggg actttctcca
     3901 tcctggccgt cgctttcgcg gcggcgcacc tgctccttga ctcggccgga cttcacgccc
     3961 ttctgcaacg gacgggcatc gggattccct atccccgcct gctggcaatc cgggcatcgt
     4021 catacctgct cggtttcatc aactataacc tggcgttcgt gctgatcgcg gcggccgcgg
     4081 ccaggggccg gtctgtcagg tggcaatcgc ttgcaagccc gttcatagcc ctgaacctga
     4141 tcgacctttt cgtcgtgtcc gcgatactga tcgccggtct cgcctcgggt tccggagacc
     4201 acctgaaggc cggcgtgccc acccttctgg cggttggagc gatactcggc ctgctggggc
     4261 tgcccgccgt catggctgcg gcagcccatg cgcgggcagg gcggcggaag gatgcgccct
     4321 ccggtcttct gcacaccttc cgagcttccg ggccggccgg ggtggccgcc gtccttggat
     4381 tgcgggtgat cctgcttgcg ttcgacatgt cgggcgactt cctgatgctc ggaacgttca
     4441 acctggacat tcctgtcggc gtattcatgc agcttttcgc cgttgacagc ttcgcgtgcg
     4501 tccttccgat caccgttgcc gggatcggac ccaccatggt tctcatgagg gcatttttcg
     4561 aaccctatgt cacgcaggcc ggcggttcga cggctgccat agacgcgttc tcggcaagcg
     4621 ccacggcgtc ccttgcggtg attcggacgg tgacggcgct ctgctgcatg cctttcgcct
     4681 tgaaggcagg cctgggtcgg acaattaaag aaatccgtgg cgaccagtga cagcgaccac
     4741 tgtccggaac ggagggatca ggccttcaac ctttcggcaa gcacttcgga cacgtcaaag
     4801 gccgacacgt tctgatcgat gccttcctcg ttgatgccgt cgcgacacat caccagacag
     4861 aaaggacacg cgctgacaat gctcttcgcc ccggtttcaa caagctggtt gacccgcaac
     4921 ctgttgatgc gacttccttc cttttcgtcc cggaacatca tgccgccgcc ggcgccgcag
     4981 cacatcgcgg tcttcccgga cctgtcggcc tcgacaaggg acagtccggc acccgcaaga
     5041 gccttgcgcg gatcttcata aatcccgtta tacctgccaa ggtaacagga gtcgtggtaa
     5101 accaccgcat cggacttgcc gccttcgaat gacagccggc cttcggcaac cagggcggcc
     5161 agcagcgacg tatggtgaat aacttcgaaa gcgccgccga actgcggata ctcgttcctg
     5221 atcgtgttga atccgtgggg acacatcgta acgatctttc ggaccccgta ttccttgaac
     5281 acctcgatat tctgacttgc ctgggcctgg aacaggtatt cattgccaag ccgcctggcc
     5341 gtatccccgc aacaaccctc ctccggccca agtatcgcga agctgacgcc cgccgcgttc
     5401 agcagcttga cgaaggccct ggccgccttg cggtttcgat cgtcataagc cgcggaacat
     5461 cccaggaaga agaggtattc ggcatcgggc ttgtcggaaa gaagggggac gccaagttcc
     5521 tcgaccatcc acagcccgcg ttcacccgaa ggcagtcccc aggggttgct cttgttttcc
     5581 aggttcttct ggcaagtcgc cagttcggga tccatcgacg cctccatcag cgtcagatag
     5641 cgccggtaac cgattatctt gtccacgtgg gtgatcgtga cggggcactg ttcctcgcag
     5701 gatctacacg tggtgcactc ccagatgact tcgtcacgga tggcgtcaat cagggaacga
     5761 ccatcctccc tggccttctc gccaagatcc ccgccggaaa ggatggcctt ttcattttcc
     5821 cggacgtaat ccctcatgtc acagatgagt cgcttggggt tcaactgctt gtccgtgcag
     5881 acggccggac agttgaccgt gcaccttcca cactcggtac acgaaaagcc gtccatcatc
     5941 tgcttccagg taagctcggt cgggcgaagc acgccgaaag tctcctgttc ctcgatgtcg
     6001 cgaatcggct cgatggcgcc aggatgcgtc agcctcatga agaagacgtt cggaatggcc
     6061 aggatgacgt ggaagtgctt ggccagcgga agctcgttca ggaacaccag aagcacgcca
     6121 acgtgcgacc agaagaagac ctggatcaga accttgaggg ttcctggcga aagaccgtca
     6181 agccagccgg ccaccatggc gccgatcggc gaccactccc tttcgtgagc gattttggaa
     6241 gccatggcgt ccggaatcac gccgcctgcc gacagcagcg cgaaccttga cgcatcatag
     6301 atgaagtcgg aaaccatcag atacgatatg aaacccagga taagaaaggc cgaaaatgaa
     6361 ttttccagac gcttcggcct gacgacaagg cgcctgaaga atgcccaggc aatcatcgcc
     6421 agcacgacca gttcggtgac gtccttcaac agggcgtacc agccgtccag cgtcggacag
     6481 aaccagaaaa gcgtccacga ggaccactcc ggcgcatacg cgcgcccgat cagggaagcg
     6541 gatctgaaga gcagaaccag gaagccccag aaaatgaaag catgcatgat gccggggcct
     6601 ggttccttga acatgcggct ctgggcaaag gccaccttca gcatttcggc aaaacgccgg
     6661 gccggcctgt cccatcggac ttcgggtttc cccgcgagca tcacgcgaat acgctttaac
     6721 atgctgacgg agaaaaatgc cagcgcaacg accagcatca gggacatcgc aatcacgctt
     6781 gctatcgaaa cagacattgg tcactcccct tgttaaggcc cggcaggccg caggaagaat
     6841 caagcacggc gcagcagcac cgcggcaaac acaggagttc cggcagccgt gagcctgacc
     6901 tcccggtcgg tcaacggcat cgccgcgatg actgcttcat cgtcagtcag acgatcatac
     6961 atgcccgtgg aaagaaaagc cgcaacaatt tcatgcaggt atggctcgac atcgctcttc
     7021 aggtgaagga tgcccccaga gcgaagcgca ttcgcaatgg tgatccccgt caaggcgccg
     7081 tgccttctgc tggcgtgctt cttcttccac cacggatccg gaaagagcag gtacgccgcg
     7141 tccgccgatc ccggatcgac cagcatcgga atggttcgcc gggcatcgcc ccaggccatg
     7201 aaaagattcg acgcgccctt cttttcagcc cgcttcaacg catccccgca aagaccaagg
     7261 ccggtctcga gaccaaggca aaccgcatcc ggacgagaca gggcgacctc gcacaacagg
     7321 cgcccacgtc cggaaccgac ctcaactacc acgggacgac catccagatg cttcctgaaa
     7381 ttctgaaccg tatctggccg ggacggcagg atcaacctct caaatctctg gaaatattca
     7441 ggaatcttat ccagaacaag ccgaccctcc ccgccgatta ttgcctcggg ccccagatct
     7501 tctccttcga atgcatgaaa ctgcctttcg gacacnnnnn nnnnnccccc ttcgattctt
     7561 tcaacgccga accccttcct gggctggccg ccgcggcctg gatgccatcc aaacgcccag
     7621 ttcgacgcca ggccgccagg ctgggaatct atcacatacc ccgggggcgt gacagacgac
     7681 gagagcctca ttgcttcgcg tccttccaat aagtacggtt attaaataca atctggtgtg
     7741 tttgttggaa gaggcaagcc atgcgtccag tcattactgt tttttcggcc ctggtcgcca
     7801 ttcatatggc cggttgcggc ggtggctccc agcctggcga ggacgtcaat attcctgttg
     7861 acataacgct ggatcagggc ggcctcgatg cgtttgatgc ggcctacgac gagggccgcg
     7921 acgggatgac gcaggcggac ggaatcgacg cgctttccca ggacacggag gatgtcgggg
     7981 atccatgcgc catgactccg atgggatttg gctgcatctg cgacgagaac cccgattgcg
     8041 aaagcggcta ctgcatcaag atgcctcatg aaaactacct gcgatgcacg cggctgtgtt
     8101 caagcaaatg cccggaagga tgggaatgca gccaggtcct gcttggcggc ggcgactcgg
     8161 catacgcatg catgccagac tacaaccagc tctgccagtc cgaatgctct gaaaacagcc
     8221 agtgcaccgc cgcgggtgcc ctgtgcatcc agtacggcaa caagaagtac tgcggacagt
     8281 cctgcaacca ggattccgat tgcgagtcga tgaccaggat caaggattgc gacaacaccg
     8341 gcgagtgcag tttcaagaac gaaacaacca tcgaggagtg cctcgaggat gaagtgacct
     8401 gcgaagtcat agaactggac ttcaaatgtg tcgaggcaac cagcatcgac ggcaccacga
     8461 tatcaaggca gtgcgttccg acaagcgacc actgcgaatg cgcgtcggac acggattacg
     8521 acaacgaccc caaccactgc ggcgattgcg agacccgctg cttctatccg aacgccacgg
     8581 cgtcgtgcag cggcggcaaa tgcaagctgg acgcatgcct gcggggctac gtcaacctga
     8641 acggcgtgcc cgatgacggg tgcgagtacg agtgcctttt ccaatcgacc gttgaccatc
     8701 cggatccgga atacatcgac gcggactgtg acgggattga cggcgatgtc gagaccggcg
     8761 tgttcgtgat gaacggcggc gatgacgaca acttcatcgg atcgatggcc ttcccgtttg
     8821 caacgatcgg ggctgcgatg gcgtttgcgg ctgaacagtc gccaattccg gaagtctatg
     8881 tttcgaaggg gatttaccag gaacaggtca acctggtgaa cggcgtcagc gtttatggcg
     8941 gatatgacgg cgacagcgga tggaagcgaa accttgtgac caaccagtca atcatttcct
     9001 ggaacggcca ggagacgctt gccgtgcgtg ccgtcgtggc cagaaacatc accgagccga
     9061 cgacattcga cggattccac gtgaaatcgt cccagtcgtc tcagctttcg ggttcctcgt
     9121 acggcatgca cgtcatggga tgcgacgaag acctggtgat atccaacaat tacctggaag
     9181 ccgacagcgg aacccagggc ggaaacggca acttcggcac gaacgggcag gccggtacaa
     9241 acggaaccaa gggctcggac gcattcgttt atgacggatg cggtctctgc atctgcacca
     9301 aattcgactt caacacgaac atgaagcctg ggcccggcgg tggcagcccg tgctcgatgg
     9361 acggcggcaa gggcggcgcg ggcggcaagc tcggcgacgg cggcctggcc ggtaccgccg
     9421 cgaccggttc gggcggggcc gggggtgctg gagctcccga tgaacagacc gatggttctg
     9481 tcggaaaacc gggcccggac ggaaacgacg ggaccgatgg cggaagcggc gtcgcaagt
//
LOCUS       DAOPPA010000061         9389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8280_length_9389_cov_4.050354, whole genome shotgun sequence.
ACCESSION   DAOPPA010000061 DAOPPA010000000
VERSION     DAOPPA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8280_length_9389_cov_4.050354"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKH54_03115"
     CDS             complement(<1..1448)
                     /locus_tag="PKH54_03115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98907.1"
                     /translation="MGRTYLGRYEIDREIGRGGMAVVYRGIDTTLNRPVAVKVMHSHL
                     LKRQDARERFCREARVIAKLKHENIVEVYDYSGNLPQGAAPDSEDALIVTELVEGISL
                     AEYLAMHGPMIPEVAAMVTAVVAGALEHAHANGVVHRDVKPENIMVGAGGVLKLMDFG
                     IAHVVDMEHLTVTGAIVGSPAHMSPEQVDGKALDARTDVFSLGTLFFLLSSGSLPFNS
                     DTPSGLLRAISEARVPDIRGRVRGFPDDLWAILARMMARDADRRFQSAGEVAESIAGV
                     LASVGIGPLPEEMKRFFSAPAVQSVEMRHRVCQGRVALGRRYLAAGRQAMAIREADTA
                     IQILPESEEAIRLMSAVRKSVKRVRLIRIAFFVGVIGLLSAGALLVMSRQRGYPGNVD
                     QADFSAAMSIQRRVAFEMGPSRRGLDGPLPARIRPMIRVRPYGAAPGQQASDVQVQPE
                     KVEPQAAPQAAPQVVPKPEAFPISIHAYPPAV"
     gene            1633..1848
                     /locus_tag="PKH54_03120"
     CDS             1633..1848
                     /locus_tag="PKH54_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOC98908.1"
                     /translation="MSEEHEATGMTTERLEALRGEHRKLDLAIKEMTARPYLTADEQI
                     EVASLKKRKLMMKDEIFRMATALGVEP"
     gene            2071..2346
                     /locus_tag="PKH54_03125"
     CDS             2071..2346
                     /locus_tag="PKH54_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC98909.1"
                     /translation="MTKAELIDEVRKAKGVDLTKKQTEIMLNAVFEAMTAAIKKDKRF
                     SFPGFGTFAVRGRKARKGRNPQNGAEITIKASKTVGFKPAPKLKSVL"
     gene            2503..3606
                     /locus_tag="PKH54_03130"
     CDS             2503..3606
                     /locus_tag="PKH54_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98910.1"
                     /translation="MTSSPSILLEHLSGTDARTIAVAGCSKNAGKTTAMNVLTGTWAG
                     SVTGLMSIGIDGEDSDFWLGVPKPRISALPGTLFATARRALEAAVGDCSIVLDAGIST
                     PLGELVIARTTGPAQVLLAGVRHKRDVAMVRDAMFRIGAARVVIDGAYQRLMSADPAI
                     SDGLILATGAILGPRPADVVERTVSFVERIRVPATQDALFLSAIELACSTGRAVAATA
                     DGRLVPAADEGCDAAVRTCAEFADTLVALAMPGAVPRGFADLLTSITGIHGSLALVVR
                     DATRLFIQGPEFRRFHGRGHRIFGEKSVNLLAIAVNPIAVTGGGMSSAELTAALSEAI
                     PDVPVFDFLKAASDGDHGSTNDTVPQGTRTFRS"
     gene            3609..5180
                     /locus_tag="PKH54_03135"
     CDS             3609..5180
                     /locus_tag="PKH54_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOC98911.1"
                     /translation="MKTSKLGLDFEKVDAARAAASEIAASVMDFAAKRTTTSVERATL
                     RMMGVEGADAEGVHWVNRVVDSPTGRDLLAHGLSVPFADAVARTGMDASETARAVAAG
                     SVEMAMPSGEALEHAVAAAEDLAADSFRRIRTQRSRRQESLAELGEGERPLLYVIVAT
                     GNIFEDVDQARVAAKAGAQVIAVIRSTAQSLLDYIPEGPTTEGFGGTFATQANFRIMR
                     DALDEVSREEGRYIRLCNYASGLCMPEMSCLGAMERLDVMLNDALYGILFRNINMQRT
                     LTDQRFARLAAAAGGFIINTGEDNYLTTADAAKSEHTVLASDLINEQIALRCGLQPEQ
                     LGLGHAMQMNPWSEDMFLKEVAMARLVREVFPGCPIKYMPPTKYMTGNIFRGHVQDAL
                     FNMAAVMTDQGIHLLGMMTEAMHTPHIHDRYLALESGAMIRQAARHLGDEIEFKEGGI
                     VRGYAAEVLENTCALLEQIRREGLFDAISRGVFADVRRHMDSGRGLDGVALRADDYRN
                     PVESMLSLMAGVKGE"
     gene            5180..5962
                     /locus_tag="PKH54_03140"
     CDS             5180..5962
                     /locus_tag="PKH54_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOC98912.1"
                     /translation="MNMESTKNAVFPKGVDPTRIKPYGDTMNDGQVQLSFSLPVPMGP
                     EAREAARRIAGRMGLKEPSIYHETDLGHGFSYFIVYGKCTNTIDFSAIVVPKIDMEVM
                     GFHEIEHFMEKHFDRRIRVVGACTGSDAHTVGIDAIMDMKGYNGEYGLERYPTFETIN
                     MGSQVSNETLLARIVEFKADAVLVSQVVTQKDVHLSNLAELVDMVEAQGVRQDLLMIC
                     GGPRISHEVALELGFDAGFGPGTKPNHVAGYIAQELVRRRRS"
     gene            6177..6395
                     /locus_tag="PKH54_03145"
     CDS             6177..6395
                     /locus_tag="PKH54_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98913.1"
                     /translation="MRGTQTGASSSPKCRTFDCKFYCRAKRRDVDTESCINDFVTANA
                     FENKRSACFRCHQGRKVREEFAGTLGNT"
     gene            complement(6413..7633)
                     /locus_tag="PKH54_03150"
     CDS             complement(6413..7633)
                     /locus_tag="PKH54_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOC98914.1"
                     /translation="MFRERKRIFVVGYHQSPFGKLGAMSVPAMIGAAAEGAAADAGIE
                     TASIDVGSIAGLIVPILNDQTLMGGLLAAVPGMAGKNIECVENACASGGQAILSVVYK
                     LLLGFGDVGIAIGIEKMRNDEGKMDGVLIGKALGTASHPENREGKVFIFPHLFAEVMD
                     RYMKEWGVTEEELAHVPVTMYRHANMNPLAQMNKVQMSVDKVMTLDGPNRYVVDGLPL
                     KTFECSQVSDGYAAIILATQEGVDRLGVAPDKMVELVGFGAAVDPLSVTGRDVIRPAA
                     AYKAMNAAYDMAGIHAENIDVAEVHDCFGIMGAIAVEILGLADPGKGVFYYVDGRARI
                     DGNGVPINTSGGLIAKGHPVGATGVAMVGWNYRQLTGQVPDGLKVKDPEYAASFNIGG
                     PICASVTTVLRKVR"
     gene            7832..8383
                     /locus_tag="PKH54_03155"
     CDS             7832..8383
                     /locus_tag="PKH54_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98915.1"
                     /translation="MKKLSLVLVCAFAMVFAVSCAKKATPDACKEACAKQAELVKAAQ
                     PQEAVADPVQKIADEFQVKMTEMETAKGADIQAIQNECDTAAANLTKEEDKAKATEDC
                     SAKKNAKAEEWAPKFAELAAQKDAAVAAAQKAKDEAAAAAAAEAEAKLAACADECVKA
                     RCTEAKVTCQIAATNLDEFNACK"
     gene            8505..>9389
                     /locus_tag="PKH54_03160"
     CDS             8505..>9389
                     /locus_tag="PKH54_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98916.1"
                     /translation="MRTFISFCVPVIAILVVATGCEGRRVTHEQDNGGIPDGYSFDLA
                     HYDLVGIDLNGLDLNSLDIRLPDYGGDAFEQNGPIARLQKSDLSVICPPDGQAEPPGF
                     QNGETDILMDVVVTAPKQEASSTLDRYFVAEATLSRATPWTGAAMTIKKELGLPDFNV
                     GDVLSVTASHLEYYCLTQLTASAATRIATDQPLEPVDVDPAEIGSQDPATAEKWEGVL
                     VRLPDVKITDAAKPYGEFEVTGGVTIKPSFGITYKPVADEEFDEIVGVVDYSYGKYVV
                     LPRSNQDLVLKGGNTPDVI"
BASE COUNT         2041 a         2855 c         2776 g         1717 t
ORIGIN      
        1 tggactgccg gcggataggc gtgaatcgaa atcgggaacg cttccggctt gggcacgacc
       61 tggggcgcgg cttggggcgc ggcctggggc tcgacctttt ccggctgcac ctggacatcc
      121 gacgcctgct ggcctggcgc tgctccgtac ggccgtaccc tgatcatcgg gcgtatcctc
      181 gcgggaagcg gaccgtccag cccccgacgg gaaggcccca tctcgaacgc aacccgccgc
      241 tggatcgaca tggctgcgga gaagtcggcc tgatcgacgt tgccgggata tccacgctgt
      301 cggctcatca ccagcagagc cccggccgaa agaaggccga ttacgccgac gaaaaaggcg
      361 atccttatca acctgacgcg cttgacgctt ttgcggaccg ccgacatcag gcgaatggcc
      421 tcttccgatt ccggaaggat ttggatggcg gtgtcggctt cgcggatggc cattgcctgg
      481 cgcccggcgg caagatacct gcgaccgagg gcgacgcgtc cctgacagac gcgatgcctc
      541 atttcaaccg actggacggc gggagccgag aaaaagcgct tcatctcctc gggcagaggg
      601 ccgatcccga ccgaggcgag aacgcccgcg atggattccg cgacctcgcc ggccgactgg
      661 aatcgccggt ccgcatcccg ggccatcatc ctggcaagaa tcgcccacaa gtcgtccgga
      721 aagccgcgaa cccttcccct gatgtcgggt acccgggcct cgctgatggc gcgaagaagg
      781 ccggatggcg tgtccgaatt gaacggaagc gaaccggaag aaagcaggaa gaacagcgtg
      841 ccaagcgaaa agacgtccgt ccttgcgtca agggcctttc cgtcaacctg ctcgggcgac
      901 atgtgggccg gcgagccgac gatggccccc gtgaccgtca ggtgttccat gtcaacgaca
      961 tgggcgatgc cgaaatccat cagtttcaga acgccgcccg cgccgaccat gatattttcc
     1021 ggcttgacgt cccgatgcac gaccccgttg gcatgtgcgt gctccagggc gccggcgacc
     1081 acggcggtca ccatggcggc aacctccgga atcatgggcc cgtgcatcgc caggtattcg
     1141 gcaagggaaa tgccctcgac cagctcggtg acaatcaggg catcctcgga atcgggggcg
     1201 gcaccctgcg ggagatttcc cgaataatca taaacttcga ctatgttttc gtgcttgagc
     1261 ttcgcgatga cgcgcgcctc gcggcagaag cgttcgcgcg cgtcctggcg cttcagcagg
     1321 tgactgtgca tcaccttgac cgcgaccgga cgattcaggg tagtgtcgat cccgcgataa
     1381 acaacggcca tgccgccccg gcctatctcg cgatcaattt catacctgcc aagatatgtc
     1441 cgacccatcc gccggcccct cggaaacgcc ccgggattta tccggattca ataccacccc
     1501 gaacacacat gttggaagaa aaaaatttaa tcgcccggcc gccgctggcg atgatgcgaa
     1561 gggcggcgtc ttgaccgccg cgccgctgtg gtctaaattc caaaattcag gctcttgcat
     1621 aagaggtggc cgatgtccga agaacatgaa gcgacgggaa tgacgaccga aaggctcgag
     1681 gcactccgcg gcgaacaccg caagctggat ctggcaatca aggaaatgac cgcgcgcccg
     1741 tatctgacag ccgacgagca gattgaagtt gcaagcctta agaaacgcaa acttatgatg
     1801 aaggacgaaa tcttcaggat ggctaccgcg ctgggcgtcg agccctgatt tcacccttca
     1861 ataaaatcaa ccacttacat caccggaatt atttaatgcc gcgaattcgc tgaattaagt
     1921 gaaaaaaacc tcttgacaag ccttcgcgtc aaaagtgaaa ctgcaccatg ctcactactg
     1981 agcggctgtc aggcagctgc gcctgacggg gatttgaagg ggtgtgttac acctgagggg
     2041 ctattgttgc catcttaagg aggaacaaag atgaccaagg ctgaactgat cgatgaagtc
     2101 cgcaaggcaa agggcgttga tctgaccaag aagcagaccg aaatcatgct gaatgcggta
     2161 ttcgaagcga tgaccgcggc gatcaagaag gacaagagat tctccttccc gggattcggc
     2221 acattcgcag tccgcggccg caaggcgcgc aagggacgga atccccagaa cggcgccgaa
     2281 atcaccatca aggcgtcgaa gaccgtcggc ttcaagcccg ctccgaagct caagagcgtc
     2341 ctgtaaccgg aattcgcaga tttcagccgg tgtcgatggc gccccctggc gcccggcact
     2401 ggtttattcc ccggcccgac acccggcctt ggcagaaccg ccttcatcga atacgatttg
     2461 acgcgtaatg tcaggggcgt gaccggtgcg cctgtgtgcg tgatgacgag ttccccttcc
     2521 atcctgcttg aacacctttc cggaacggat gcccggacga ttgccgtggc gggctgctcc
     2581 aagaacgcag gaaaaacgac ggcgatgaac gtcctgacgg gaacctgggc cggaagcgtg
     2641 acgggcctga tgtcgattgg aattgacggc gaggactcgg acttctggct cggagttccg
     2701 aagccaagga tttcggccct gcctggaacc cttttcgcga ccgcccgccg cgcgctcgag
     2761 gcggctgtcg gggactgctc gatagtcctg gacgccggca tatccactcc gctcggcgaa
     2821 ctggtcatcg caaggacgac cgggccggcg caggttctgc tggccggagt caggcacaaa
     2881 cgggacgtgg cgatggtaag ggacgcgatg ttccggattg gcgctgcaag ggtcgtgatt
     2941 gacggcgctt accagcgcct gatgtccgcc gatccggcga tttccgacgg actgatcctg
     3001 gcgaccggtg caatcctggg tcccaggccg gcagatgtgg tcgaaagaac cgtttcattc
     3061 gtggaaagaa tcagggttcc cgcaactcag gatgccttat tcctgtccgc catcgaactg
     3121 gcatgttcga ccgggcgggc ggtggccgcg acggcggacg gacgcctggt tccggcagcc
     3181 gacgaaggtt gcgatgccgc cgttcgaact tgcgccgagt ttgccgacac gctggtcgcg
     3241 ctggccatgc ctggcgcggt tccgcgaggg tttgccgacc tgctgaccag catcaccggg
     3301 attcacggat cgctggcgct cgtggtcagg gacgccacca ggttgttcat ccagggcccc
     3361 gagttccgcc ggttccacgg acggggccac cggattttcg gggaaaaatc ggtcaacctc
     3421 cttgcaatcg ccgtcaaccc aatcgccgtc acgggcggcg gaatgtcttc cgctgaactt
     3481 accgccgcct tgtcggaggc gattcccgac gtgccggtat tcgatttcct gaaggccgca
     3541 tcggatggcg accacggctc gaccaatgac acggtgccgc agggcaccag aacgttccgg
     3601 agttgaagat gaagacaagc aagctgggcc tggactttga aaaggttgat gcggcacgtg
     3661 ccgcagcctc cgaaatcgcg gcttccgtga tggattttgc cgcgaagcgc accaccacat
     3721 ccgttgaacg cgcgaccttg cgcatgatgg gtgtcgaggg agccgacgct gaaggcgtcc
     3781 actgggtgaa ccgcgtagtc gattccccga cgggacggga cctgctggcc cacggcctgt
     3841 cggttccttt cgcggatgcg gtcgcgcgaa ccggcatgga tgcgtccgaa acggcaaggg
     3901 ccgttgccgc cggctcggtc gaaatggcga tgccgtccgg cgaggccctt gaacacgcgg
     3961 ttgccgcggc cgaagacctt gccgcagaca gtttccgacg gatccgaacc cagcgctcca
     4021 ggcgccagga atcccttgcc gaactcgggg aaggcgagcg ccccctgctt tacgtcatcg
     4081 tggccaccgg caacatcttc gaggacgtcg atcaggccag ggtcgcggcc aaggccggcg
     4141 cacaggtgat cgcggtcatc agatcaacgg cccagagcct tctggactat attcccgagg
     4201 gcccgaccac cgaaggattc ggcggcacct tcgcgaccca ggccaacttc aggatcatgc
     4261 gcgacgcgct tgacgaggtc agccgggaag agggccggta catccgcctg tgcaactacg
     4321 caagcggcct ctgcatgccg gaaatgtcct gcctgggcgc gatggaacgg ctggacgtga
     4381 tgctgaacga cgcactctac ggcatccttt tccgcaacat caacatgcag cggaccttga
     4441 ccgaccagcg cttcgccaga ctggccgctg cggctggcgg attcatcatc aacaccggcg
     4501 aggacaacta cctgaccacg gccgacgccg cgaagtcgga acacacggtc ctggcgtcgg
     4561 atctgattaa cgaacagatc gccttgcggt gcggactcca gccggaacag ctcggacttg
     4621 gccatgctat gcagatgaat ccgtggtccg aagacatgtt cctgaaggaa gtggcgatgg
     4681 cccgactggt tcgcgaggtc ttcccgggat gccccatcaa gtacatgccc ccgaccaagt
     4741 acatgacggg aaacatattc cggggtcacg tccaggacgc gctgttcaac atggccgccg
     4801 tcatgaccga tcagggaatc cacctgctcg ggatgatgac cgaagcgatg cacacgcccc
     4861 acatacacga tcgctacctg gcgctcgaat ccggggcgat gattcgccag gccgcgcggc
     4921 atctggggga cgaaatcgaa ttcaaggaag gcggcatcgt tcgcggatac gccgccgaag
     4981 tgcttgaaaa cacatgcgcg ctgcttgaac agataagacg cgaaggtctt ttcgacgcga
     5041 tatcccgcgg agtgtttgcc gacgtccggc gccatatgga ttccggacgc gggctggatg
     5101 gcgtagcgtt gcgcgcggat gactaccgca acccggttga atcgatgctt tcgttgatgg
     5161 ccggcgtgaa aggggaatga tgaacatgga atccacaaaa aacgctgtat tcccgaaggg
     5221 cgttgaccca accaggatca agccgtacgg cgacaccatg aacgacggcc aggtgcagct
     5281 ttcgttcagc ctgcccgtgc cgatgggccc cgaggcccgc gaagccgccc gcaggattgc
     5341 cggaaggatg ggcctgaagg aaccttcgat ttatcatgaa accgatcttg gtcacggatt
     5401 ctcatacttc atcgtttatg ggaagtgcac caacaccatc gacttctcgg ccatcgtcgt
     5461 gccgaagatt gacatggaag tgatgggatt tcacgaaatc gaacacttca tggaaaagca
     5521 cttcgaccgg cggattcgcg tcgtcggcgc ctgcaccggc agcgacgcgc acacggtcgg
     5581 catcgatgca atcatggaca tgaagggata caacggcgaa tacgggctcg agcgataccc
     5641 gacattcgaa accatcaaca tgggcagcca ggtatcgaat gaaaccctgc ttgccaggat
     5701 tgtcgaattc aaggcggatg cggttctggt aagccaggtc gtgacccaga aggacgtcca
     5761 cctgtccaac cttgcggaac tggtcgacat ggtcgaggcc cagggggtgc gccaggatct
     5821 gctgatgatc tgtggcggcc ccaggatttc ccacgaagtc gccctggaac tcgggttcga
     5881 cgccgggttc gggcctggaa ccaaaccgaa ccacgtggcc ggctacatcg cacaggaact
     5941 ggtcagaaga agacgatcct gagatagccg acataatctg ggatattttt ctaaaaattt
     6001 tttcgagctt gagccaatac caaatctagc cgccagataa accacctaaa ttaaacaaca
     6061 attcttattc ggataaaaaa catcaaatta gcttgacata cccacgaacc gcgattaaat
     6121 tcatcctagt tgtttgaatt aaacatccct gaatacgatg gacggaggag ttgaaaatgc
     6181 gtggaaccca gactggcgcc tcaagttcac caaagtgccg cacctttgat tgcaagttct
     6241 actgcagggc aaagcgccgt gatgttgaca cggaatcgtg catcaacgac ttcgtcacgg
     6301 caaacgcctt tgaaaacaag agatccgcat gcttcaggtg ccaccagggg cggaaggttc
     6361 gcgaggaatt cgccggaacc ctgggcaaca cctaggcatt gacgaacggc ggtcagcgca
     6421 cctttcgcag aaccgtggtg accgaagcgc agatcgggcc gccgatattg aatgacgccg
     6481 cgtactcggg atccttcacc ttaagtccat ccggaacctg tccggtaagc tgtctgtaat
     6541 tccagccgac catggcgacg cccgtggccc cgacggggtg gccctttgcg atcaatccac
     6601 cggaagtgtt aatcggaacg ccgttgccat ctatccttgc gcggccatcc acgtagtaga
     6661 agaccccctt tccgggatcg gcaagaccaa ggatttcgac ggcgatggca cccatgattc
     6721 cgaagcagtc gtgaacctcg gcgacatcga tgttttccgc atgaatgccg gccatgtcgt
     6781 acgccgcgtt catggccttg taggcggccg ccggcctgat gacgtcacga cccgtaaccg
     6841 aaagcggatc taccgcggcg ccgaatccga ccagttcgac catcttgtcc ggcgcgacac
     6901 ccaggcgatc gacaccttcc tgggttgcaa gtattatggc ggcgtacccg tcggaaacct
     6961 ggctgcactc gaaagtcttc agcggcaagc cgtcaaccac atagcgattc gggccgtcca
     7021 gcgtcatcac cttgtcaacc gacatctgaa ccttgttcat ctgggccagc gggttcatgt
     7081 tcgcatgccg gtacatcgtc acgggtacgt gggccagttc ctcttcggtg acgccccatt
     7141 ccttcatata tcgatccatg acctcggcaa acaggtgggg gaagatgaag accttgcctt
     7201 cccggttctc gggatgcgat gccgttccca gcgccttgcc gatcaggacg ccgtccatct
     7261 tgccttcgtc gttgcgcatc ttctcgatcc cgatcgcgat gccgacgtcg ccgaacccga
     7321 gcaggagctt gtacacgacg gaaagaatcg cctggccacc actcgcgcat gcgttctcga
     7381 cgcactcaat gttcttgccc gccatccccg gcaccgcggc cagcagcccg cccatcagag
     7441 tctgatcatt aagtatcgga acaatcaggc cggcaatgga tcccacatcg atggaggccg
     7501 tctcgatccc ggcgtccgcc gcggcaccct cggcggccgc gccaatcatc gccggaactg
     7561 acatcgcccc cagctttccg aatggacttt gatggtaacc gacaacgaaa atcctcttcc
     7621 tttcccggaa catgtgagac ctcgattcaa gctgcaaaaa tcttacatca aacgggaaaa
     7681 attcaacaca caagtcttta ccgtttgaag tcgttgacat ttcgatgaat tcggacataa
     7741 tggtcgcact tcgtatgaga cttcagtccc aatggtggga cggattgctg aagtctcggt
     7801 cccatggtgg ggcagaagga ggaatattac aatgaagaag ctgtctttgg ttctggtgtg
     7861 cgcgttcgca atggtttttg ctgtttcctg tgccaagaag gcaaccccgg atgcctgcaa
     7921 ggaagcttgc gcgaagcagg cggaactggt caaggccgcc cagccgcagg aagcggtggc
     7981 tgaccccgtc cagaagatcg ccgatgaatt ccaggtcaag atgaccgaaa tggaaacggc
     8041 caagggcgcc gacatccagg cgatccagaa cgaatgcgac accgccgccg ccaacctgac
     8101 gaaggaagag gacaaggcga aggccactga agattgcagc gcgaagaaga acgcgaaggc
     8161 cgaagaatgg gccccgaagt tcgccgaact ggccgcccag aaggacgccg ccgtcgccgc
     8221 cgcccagaag gcgaaggacg aagccgctgc cgccgccgcc gccgaagccg aagccaagct
     8281 ggctgcctgc gccgacgaat gcgtcaaggc ccgttgcacc gaagcgaagg tcacctgcca
     8341 gatcgcggcg accaacctcg atgaattcaa cgcctgcaag tgattcgcga ctgattcccg
     8401 tccgtaatcc ccgaccatct ccataagcca tccggacaca atccgattga caccgggccg
     8461 cttcgaaacg aaactggttt gcggcatttt ccggagtcat gcgcatgcgg actttcatct
     8521 ccttctgtgt tccagtcatc gccattcttg tcgtggccac gggttgcgag gggcgccggg
     8581 tcacccatga acaggacaac ggcggtattc cggacgggta ttccttcgac ctggcccatt
     8641 acgatctggt cggcatcgat ctgaacggac tggatctgaa cagccttgac atcagactgc
     8701 cggattacgg cggcgacgcg tttgagcaga acggcccgat tgcgaggctg cagaagtccg
     8761 acctgagcgt catctgcccc ccggacggac aggccgagcc ccccggcttc cagaacgggg
     8821 aaacggacat cctgatggac gtggtcgtca cggcgcccaa gcaggaagcc tcatcaactc
     8881 ttgaccgcta cttcgtggcc gaggccaccc tttccagggc aaccccctgg accggtgccg
     8941 ccatgacgat caagaaggaa ctgggccttc cggatttcaa cgtgggcgat gtcctttccg
     9001 tgacggcaag ccatctggaa tactactgcc tgacccagtt gacggcctca gccgccacgc
     9061 gcatcgcgac ggatcagccg cttgagccgg tcgatgtcga tccggccgaa atcggatccc
     9121 aggatccagc aacggcagag aagtgggaag gcgtcctggt tcgcctgccc gacgtcaaga
     9181 tcacggacgc cgccaagccc tatggcgaat tcgaggtaac cggcggcgtc acgatcaagc
     9241 cgtctttcgg gatcacctac aaaccggtgg ccgacgagga attcgacgag attgttggcg
     9301 ttgtcgatta ttcgtacggc aagtacgtcg tcctgcccag atcgaatcag gatctggtgc
     9361 tgaagggcgg caataccccg gacgtcatt
//
LOCUS       DAOPPA010000062         9329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8348_length_9329_cov_3.851413, whole genome shotgun sequence.
ACCESSION   DAOPPA010000062 DAOPPA010000000
VERSION     DAOPPA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_9329_cov_3.851413"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1935
                     /locus_tag="PKH54_03165"
     CDS             325..1935
                     /locus_tag="PKH54_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98917.1"
                     /translation="MKYQPIVPILACLVCFGCSGTSAPVPDDTGATDTLRADSVDIAA
                     PDAVDDVAESLDVADADVADADVADADDAPDATDADDATDTTVCNEFCLGACASRRFD
                     APTGECPGFVCDLDPAISGTPHPIPGGGWLLESSPNLCVTDTASWRLAMLSTDGTFSV
                     LQQREINIMNADQTLPSLVAVQKDKYLLLKTGCPYDECREELAGQYATLSAYSFDSAA
                     ALWQIGGPELQNGNLASAVAVDSDRIWIASTQWNPGNVGSTHLVSVKFDGTVADTIEI
                     PGDGWLKAFMADDDRILLVFRSTTTSESAQTTSVYSISIDRLGNVGGPTELKTITFSS
                     SGSVVTRFLPMTSGMIGYYRIFDSATPPISPGTFVINLDGGRRWIHEGEWENGQFEFS
                     WVVHEFDDSTIGYITPSGTEYRKISADGQSSTTIQPAIPPPVDGLNWGIMGMYFLDDG
                     ILAVGAASRNSPDNEYFIARYTGDFGTLKWVRNYGSSSHGGGLRPDGDGWLLWYDRCS
                     PWIGHDGGEFSCGETCAGTYLGFDSICG"
     gene            2069..2135
                     /locus_tag="PKH54_03170"
     tRNA            2069..2135
                     /locus_tag="PKH54_03170"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     assembly_gap    2077..2086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2226..3182
                     /locus_tag="PKH54_03175"
     CDS             2226..3182
                     /locus_tag="PKH54_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOC98918.1"
                     /translation="MSFRVLIADKLDKQAVPILVDAGFEVVNKPGMNEDEIAAAIPGF
                     DAVIVRSAAKITAKIIQAADCLKCIGRAGTGVDNIDVKAATAKGILVMNVPGGNTVTA
                     AEHALAMMLSMSRMVPQADASMKAGKWEKSAFTGFEITGKTLGVVGFGKIGQIVADRA
                     LGLRMKVVAYDPIVPPFVIEAAGVTPVTTVADLMKVADIVSVHTPLNDKTRGLIGADM
                     LACAKDGVMLVNCARGNIVDEAALIEALKSGKVRSAAIDVWSAEPFAEGSVAATLAAM
                     PNVVATPHLGASTHEAQNRVALSIARQIRDFLNTGARFGAVN"
     gene            3222..4514
                     /locus_tag="PKH54_03180"
     CDS             3222..4514
                     /locus_tag="PKH54_03180"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOC98919.1"
                     /translation="MTDCLTIVGVQWGDEGKGKITDLLADRADIVARYQGGANAGHTV
                     VVEGRKIVLHQIPSGILNPRSLCLIGPAVVIDPQTIINEFQELIDAGWIVNPERLRIS
                     SQAHVVMPYHKTLDKLREAARTKGKIGTTGRGIGPAYEDMVSRAGIRVRDFCNEERLA
                     ERLHALLPERNAVMQFLGGEPVQVESVLEWARPLAARISDYQADTGKLLADCIGRGGK
                     VLFESAQGTLLDVIHGTYPFVTSSFTSAPAAFSLSGIGIARTGVVLAVVKAYTTRVGE
                     GPFPTEDFGAFGARLRENGHEFGATTGRPRRCGALDLPALRYTFRVNGVTSIALTKMD
                     VLSGFDSFPVCIAYDVPGRGRVNELVADDFTTPGIQPIYESWPGWSEDIGGVLSIESL
                     PANCRAFIGRLERELGVPIDIVSTAPDRESTIVVNPVW"
     gene            4596..5690
                     /locus_tag="PKH54_03185"
     CDS             4596..5690
                     /locus_tag="PKH54_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC98920.1"
                     /translation="MSDNGEQDRRQTPSPGHILSTFRLLIVAERHLGTKLTGILSPFG
                     YHVTCIDDPNVARQTIGLMPPDLLILSGNFPKEESWNILRAVRTDRITQQLPVLMFFH
                     SIDSENEVRAFQYGVDEIVDSSSQDIAIRARVRVLLRISAYRRRIENEKRRLELRVQE
                     RTKELMEITFSTVAALEKAAEFSDHETGQHMNRVAEYSCVLARELDLGSELIEKIRVY
                     APLHDVGKVGISHDILKKEGILSADEFEEMKRHTVLGYELLKAARADPVACNIALCHH
                     ERFDGSGYPNHLQANEIPIEARIVSVADVFDALITKRHYKQAMRPDMAFLCITSEMKN
                     HFDANVLRAFAVRFDEIRDIWARLDGTLGG"
     gene            5756..6508
                     /locus_tag="PKH54_03190"
     CDS             5756..6508
                     /locus_tag="PKH54_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98921.1"
                     /translation="MKNLDQISAEELISALLMRFPDSFAEAGVKRGEIRQALADFDPE
                     SNELASSILETMRLLRLRAGQLKVSDLPTEVQEVNGDASQAPTEQLPPADVPEEMPQA
                     ADEVASEVTQPLPHKIPGRWTDRERLLYRDIIHLFELGDHGGAMASLERLFMLCPDAT
                     ELSTFLGKNEATLLRLYRDQMGTMDRVTIPSRSRKPIRIPTPDADLMLKLIRMSDGHR
                     PIRDFVKKAESPELHVLLTLSHLVRSGYLELA"
     gene            complement(6654..8936)
                     /locus_tag="PKH54_03195"
     CDS             complement(6654..8936)
                     /locus_tag="PKH54_03195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC98922.1"
                     /translation="MADVQDSAVVQNPEQARMRPRQFGKYTLLEKIGTGGMAEIFKAV
                     SRGAGDFQKVLVIKRILVRYSRDPAFVKMFKDEALITAPLQHANIVSIYEFDQVDGQY
                     YLAMDLVNGVDLQAVMARANKIGATIPPGVALFIIGEVCKALWYAYNARDAYGKPLKI
                     IHRDVSPSNILISFDGEVKVTDFGVAKAANSKNEGQTGVLKGKLGYMSPETVLGREID
                     NRSDLFSLGIILFEILTLKRMFLGKTDLQTLINIRDADADKRLSKHPEIDDGVASIIR
                     KSLAREPDDRYPGAQEFLDDIQDYLFGRRLRVSQLDVANLMKELFAEKVERELLPLGS
                     EESTTGVSGRVSTTAIRNAPSISEDEYADEDDDSRDDEDRDQPEPEPEPVVEPPSRAG
                     GRRKAVAVPPPEDAPEHEQSQPTIGMSAGSDESGDLPIDDKSPEPSRESTRNTKVKPE
                     VATFRIRDAAGNIFGPVSFENVLSLLRSRALTEDELVSVDNGDWQRLGDVSIVQSHLG
                     QIRPVIDRNSMLFDAQIDRRFNVRMLTRVCRRQVLSGLLVFKKGVHQKEIYFVKGRPK
                     VVFSNLKSELLGEYLVRRQLVAREKLADALKDMASGGGRLGDSLINHGAVGSHELALF
                     LADQFKDRFLDIFTWEEGWCGFFEGVKVPQGVVQHDMDPMELISQGVRTMIPPQMINA
                     WMSEYAQQRMVFASDAKVTVEELKLIPRELRVVNLIGARDTIRALLDGLPQGGDWPFV
                     VNRVVYLLLETGIVRFRTGR"
     gene            complement(8970..9209)
                     /locus_tag="PKH54_03200"
     CDS             complement(8970..9209)
                     /locus_tag="PKH54_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98923.1"
                     /translation="MKKRLLLLACAILPLVSVSCTEPCSRLADHVCLKRGGGSTQCQE
                     MKEFADQAGLAERHYCQSALAVIKSLGSGVQGGRD"
BASE COUNT         1972 a         2793 c         2615 g         1939 t
ORIGIN      
        1 gacagaatct tcagcttcgg cttcgccgcg gccgcctgtt tcgtgctttt cgcgcacccg
       61 gcgaccccgg ccagcgccag caaaagtgcc atgaaccaaa gcacccacaa acgtccgcga
      121 tagttcataa tcagaaacct tcgtggctta cccggcggat tatatcgcca atcccccggc
      181 gaacaaacgc cttcggccat gacaccgacg cataggccgc catcgcttcc gatttgattc
      241 gcatttgcta aagttataac atcaaatcct taaccattga gaccgggtta taaccgtcgt
      301 ttcagcctta cgggagtgcg ctgcatgaaa taccaaccga tcgtcccgat acttgcctgc
      361 ctggtctgct ttggatgttc cgggacatca gcgccggttc ccgatgacac gggcgcgacc
      421 gacacactgc gtgccgactc ggtcgatatc gcggcaccgg acgctgtgga cgatgtcgcc
      481 gaatccctgg atgttgcgga cgccgatgtt gcggacgccg atgttgcgga tgccgatgat
      541 gcccccgacg ccactgatgc cgatgatgcc accgacacga ccgtgtgcaa tgaattctgc
      601 cttggcgcgt gcgcttcccg gcgtttcgac gcacccaccg gcgagtgtcc aggatttgtc
      661 tgcgatcttg atcccgcgat ctcgggcacg ccgcacccaa tccccggcgg gggctggctg
      721 cttgaatcca gcccgaacct ttgtgtcacc gacacggcgt catggcgact tgcgatgctt
      781 tcgaccgatg gaaccttcag cgtccttcag cagcgcgaaa tcaacatcat gaacgccgac
      841 cagactcttc catcgctggt cgcggttcaa aaagacaagt acctgctgct gaagactggc
      901 tgcccgtacg acgagtgccg ggaagaactg gccggacaat atgcaaccct gtccgcgtac
      961 tcattcgaca gcgcggcggc tctgtggcaa atcggtggtc cggaattgca gaacgggaat
     1021 cttgcgtcgg ccgtggccgt cgattccgat cgcatctgga tcgcatcaac acagtggaac
     1081 cctggcaacg tcggcagcac ccacctggtg tccgtcaaat ttgacggaac ggttgcagat
     1141 acaattgaaa tccccggtga cggatggctt aaggccttca tggccgacga tgacagaatc
     1201 cttctggtat tcaggtcaac gacgacaagc gaaagtgcac agacgacttc cgtctattca
     1261 atcagtatcg acagactggg caatgtaggc ggcccgactg aacttaagac aatcacgttt
     1321 tcatcgtcgg gctccgtggt tacgcgattc cttccaatga cttccggaat gatcggctat
     1381 tacaggattt tcgattcagc gactccgccc atatcccccg gcaccttcgt cattaacctt
     1441 gatggagggc gaagatggat tcacgaaggc gagtgggaaa acggtcagtt cgagttttcg
     1501 tgggtcgttc atgaattcga tgattcgacg atcggatata tcacgcccag cgggacggag
     1561 taccgtaaga tttcggcgga cggccagtca tcgacgacca ttcaaccggc aatcccgccg
     1621 ccggtcgatg gattgaactg gggcatcatg ggaatgtatt tcctggatga cggaattctg
     1681 gcagttgggg cggcgtccag gaacagcccc gacaatgaat acttcattgc acgatacacc
     1741 ggcgattttg gcacgcttaa gtgggtgcgt aattacggtt cgtccagcca cggtggcggg
     1801 ttgcggcccg acggcgacgg atggctgctc tggtacgatc ggtgcagccc ctggattggc
     1861 catgatggcg gggaattcag ttgcggcgaa acctgtgccg gcacgtatct cggctttgac
     1921 tcaatctgcg ggtaacagcc cgccacgaca tctttcatca tttgacatga actcaggatg
     1981 catccgcaag taatccaaac ctgtgcataa cttccccggt ttgcattgcc aatgacaggg
     2041 tggtgtgcta atctccagcc gtttgttcgg gcgattnnnn nnnnnncgtt ggtctccgga
     2101 atcaaaggcc gtgggttcga atcccgcatc gcccaccgac gaaccttggg tttccccgac
     2161 gcccgttggg ggaaggacgt gttcgcccat cggttcgcaa tcaattttcc gcattggagg
     2221 atgtcatgtc ttttcgcgtt ctgatcgcag acaagttgga caagcaggca gttccgattc
     2281 tggttgacgc tggattcgaa gtcgtcaaca agcccgggat gaacgaagat gaaatcgccg
     2341 cggcgattcc aggtttcgac gcagtcatcg tccgcagcgc agcaaagatc accgcgaaaa
     2401 tcatccaggc cgccgactgc ctgaagtgca tcggccgcgc aggcaccggc gttgacaaca
     2461 tcgacgtcaa ggccgcgacg gcaaagggaa tcctggtcat gaacgtcccg ggcggcaata
     2521 ccgttacagc cgccgaacac gctcttgcga tgatgctttc gatgtcccgc atggttcccc
     2581 aggccgacgc aagcatgaag gccggcaaat gggaaaagtc ggcctttacc ggctttgaga
     2641 tcaccggaaa gacccttggc gtcgtgggat tcggcaagat cggacagatt gtcgccgacc
     2701 gcgcgcttgg actgcgcatg aaggttgtcg cttacgatcc gatcgtcccc ccgttcgtga
     2761 tcgaagccgc cggcgttact ccggtgacaa cggttgcgga tctgatgaag gttgccgata
     2821 tcgtgtcggt gcatacgccg ctgaacgaca agactcgtgg actgattggc gcggacatgc
     2881 tggcgtgcgc gaaggacgga gtgatgctgg tcaactgcgc gcgcggcaac atcgttgacg
     2941 aggctgccct gattgaagcg ctgaagtccg gcaaggttcg atccgccgcc atcgacgtct
     3001 ggtctgccga accgttcgcg gaaggaagcg tcgcggcgac ccttgcagcc atgccgaacg
     3061 tcgtggcaac gccccacctg ggcgccagca cgcatgaggc gcagaatcgc gtcgccctgt
     3121 cgattgcgcg gcagattcgc gacttcctga acaccggcgc ccgttttggc gcggttaact
     3181 gagccggccc ttttctccca tacgaaatgc gtgagttcat gatgacggat tgcctgacga
     3241 ttgttggtgt tcagtggggt gacgaaggca agggaaaaat taccgacctg ctggcggatc
     3301 gggcagacat cgttgcccga taccagggcg gcgcgaacgc cggccacacg gtggtcgtcg
     3361 agggtcgcaa gatcgtcctt caccagattc cctccggtat cctcaatcca cggtcgctct
     3421 gcctgattgg acctgccgtg gtcatcgacc cgcagaccat catcaatgaa ttccaggaac
     3481 tgatcgacgc gggctggatc gtcaacccgg aacgccttcg catcagcagc caggcccatg
     3541 tggtcatgcc ataccacaag acgctggaca aactgcgcga agccgcaagg acaaagggca
     3601 aaatcggcac cacgggacgc ggtatcgggc cggcgtacga ggacatggtg tcacgcgcgg
     3661 gcatcagggt tcgcgatttc tgcaatgaag aaaggctcgc tgaacggctt catgccctgc
     3721 ttccggaacg caacgccgtg atgcagttcc ttggcggcga gccggttcag gtcgagtcag
     3781 ttcttgaatg ggcaaggcct ctggcggcca gaatttccga ctaccaggcc gacaccggga
     3841 aactgcttgc cgactgcatc gggcgtggcg gaaaggttct gttcgaatcg gcccagggca
     3901 cgctgctcga tgtcatccac ggcacgtacc cgttcgttac atcttcattc acctccgcac
     3961 cggcggcatt cagtctttcg ggcatcggaa tcgcacgcac aggcgtggtt cttgcggtcg
     4021 tcaaggcgta caccacacgc gtgggcgaag ggccgttccc aaccgaggac ttcggcgcgt
     4081 tcggagccag gctgcgcgaa aacggacatg aatttggcgc cacgaccggc cgtccgcgac
     4141 gatgcggcgc gcttgatctt ccggcgcttc gttacacgtt ccgcgtcaac ggcgtgacct
     4201 cgattgcgct gaccaagatg gatgtgcttt ccgggttcga ctcgttcccg gtttgtattg
     4261 cctacgacgt gcctgggcgc ggccgcgtta acgaacttgt tgccgacgac ttcacgacgc
     4321 ccggcatcca gccaatctat gagagttggc ccggttggtc ggaagacatc ggcggggtat
     4381 tgtccatcga aagcctgcct gcgaactgcc gggcatttat cgggcgcctg gaacgcgaac
     4441 tcggggttcc aatcgacatc gtatcgacag ccccggatcg tgagtccaca attgttgtaa
     4501 acccagtctg gtaaccccgg cgccacgcac ccaaaagttt taccaatgtc tttacgtttg
     4561 atacactaac tgccgtgtcg cttcggcggt gacctgtgtc cgacaacggc gaacaagaca
     4621 gaagacagac gccctcgccg ggccatatcc tttcgacctt caggctgctc attgtggccg
     4681 aaaggcatct tggcaccaaa cttaccggaa ttctttcccc tttcggttac catgtgacct
     4741 gcatagacga ccctaacgtt gccaggcaga ccattggcct tatgccaccc gacctgttga
     4801 ttctctcggg aaacttccca aaggaggaaa gctggaacat cctccgggcg gtcaggaccg
     4861 acaggattac ccagcagttg ccggtactga tgttcttcca ctcaatcgat tcagaaaacg
     4921 aagtgcgcgc cttccagtac ggcgttgacg aaatcgtgga ttccagttcc caggatatcg
     4981 cgattcgcgc ccgtgttcgc gtgctgcttc gcatttccgc gtacaggcgc cggattgaga
     5041 acgagaagcg gcgtcttgag cttcgagttc aggaacgaac aaaggaactg atggaaatca
     5101 cgttttccac ggttgcagcc ctggaaaagg ccgctgaatt ttccgaccac gagactggcc
     5161 agcatatgaa ccgcgtggcg gaatactcat gcgtgctggc ccgcgagctg gatcttggaa
     5221 gcgaactaat tgaaaagata cgggtttacg cgcctttgca cgacgttgga aaggttggga
     5281 tcagccatga catccttaag aaggaaggca tcctgtccgc cgacgagttc gaggagatga
     5341 agcgccacac agtcctcgga tacgagctcc tcaaggcggc ccgagcggat cccgtggcct
     5401 gcaatatcgc cctgtgccac cacgagcgtt tcgatggcag cggctatccc aaccacctgc
     5461 aggcgaacga aatcccgatc gaggctcgaa tcgtgtcggt tgcggacgtt ttcgacgcgc
     5521 tgatcaccaa aagacattac aaacaggcca tgagaccgga catggcattc ctgtgcatca
     5581 catccgaaat gaagaatcac tttgatgcaa acgtcctgcg cgccttcgcg gttcgtttcg
     5641 acgagatccg cgatatctgg gcacgccttg acggtacgct tggcggttga cggcccgaag
     5701 cattttcgtg ccgtgaattt attgattgcg gcttttcggt gatacgattt cgagcatgaa
     5761 gaaccttgac cagatatctg cggaagaatt gatttcggcg ctgctgatgc gatttcccga
     5821 ttcatttgcc gaggcgggcg tgaagcgggg cgagattcgg caggcacttg ccgatttcga
     5881 ccccgaaagc aacgaattgg cgtcgtccat cctggagacg atgcgactcc tgcgattgcg
     5941 ggccggccag ttgaaagttt ccgaccttcc caccgaagtt caggaagtta acggtgacgc
     6001 atcccaggcc cccaccgagc agcttccgcc ggccgatgtc ccggaggaaa tgccccaggc
     6061 cgcggatgaa gttgccagcg aggtaaccca gccactgcca cacaagattc ccgggcggtg
     6121 gaccgatcgc gagcgacttc tttaccgtga catcatccac ctgttcgagc ttggcgacca
     6181 cggcggcgct atggcctcgc tcgagcggct tttcatgctg tgtccggacg ccaccgagtt
     6241 gtcgactttc cttggaaaga acgaggcgac tctgctgcgg ctttatcgtg accagatggg
     6301 aacaatggat cgcgtaacga ttccaagccg ttcaaggaag ccgatccgca tccccacccc
     6361 ggacgccgac ctgatgctta aattgattcg aatgtcggac ggccaccggc ccatccgcga
     6421 cttcgtgaag aaggccgagt cccccgagct tcacgtgctt cttacccttt cccatcttgt
     6481 gcgttccggc taccttgaac ttgcctgacg caatcccgta agggcgaatg aaaattcggc
     6541 cgcatgcctg ccttccttgt acgggcggat tacatccgcc cgctgcgatt ctgcaaaatc
     6601 cgtaagggcg aatgaaaatt cgcccgccat gacgaacctt aaatttgcaa tgactaccga
     6661 ccagtgcgga agcggacgat gccggtttca agaagcaggt aaacgacacg gttcacgacg
     6721 aacggccagt ccccgccctg gggaagtccg tccagcaggg cccggatggt atcgcgcgcg
     6781 ccaatcaggt tcacgacgcg aagttcacgc ggaatcaatt tcagttcctc gacggtcacc
     6841 ttggcatcgg atgcgaacac cattctctgc tgggcgtact cggacatcca tgcgttgatc
     6901 atctggggcg gtatcatcgt ccgcacgccc tgggagatca gttccatcgg atccatgtcg
     6961 tgctggacga cgccctgggg aaccttgacc ccttcgaaga acccgcacca gccctcttcc
     7021 cacgtgaaga tgtccaggaa gcgatccttg aactggtcgg ccaggaaaag ggcaagttca
     7081 tgtgaaccga ccgcgccgtg gttaatcagg ctgtcgccaa gacgtccccc gccgcttgcc
     7141 atgtccttca gagcatcggc cagcttctcg cgggccacaa gctgccgcct gacaagatat
     7201 tcgccaagaa gctcggattt caggttcgag aagacgacct tgggccgccc cttcacgaag
     7261 taaatttcct tctggtgaac gcccttcttg aacaccagca gaccgctcaa gacctggcga
     7321 cgacagaccc tggtcaacat tcgcacgttg aacctgcggt cgatctgtgc atcgaaaagc
     7381 atgctgttgc ggtcgatgac cggccgaatc tgaccaaggt gactctggac gatggaaaca
     7441 tcgccaagcc gctgccagtc accgttatcg accgacacga gttcgtcttc ggtaagcgcg
     7501 cggcttcgaa gcaacgaaag gacattctca aacgaaaccg ggccgaaaat gttgccggcg
     7561 gcgtcccgaa tccggaaagt cgccacctcg ggcttgacct tggtattccg ggttgactcg
     7621 cgggaaggct ctggcgactt gtcatcaatt ggaagatcgc cgctttcatc ggacccggct
     7681 gacattccga tcgtcggttg agactgctcg tgttccgggg catcctcggg cggcggcacg
     7741 gccacggcct tcctgcgccc gcctgccctt gacggcggct cgaccaccgg ctctggttcg
     7801 ggctccggct ggtcgcgatc ctcatcatcg cgcgaatcgt catcctcgtc ggcgtactcg
     7861 tcttcggaaa tcgatggcgc attgcgaatc gcggttgttg aaacccgacc gctgacgcct
     7921 gtcgttgact cctcgcttcc aagcggcagc agttcccgct cgaccttttc cgcgaaaagt
     7981 tccttcatca ggttggcaac gtcgagctga ctgacccgca accttcgccc gaaaagatag
     8041 tcctggatgt catcaaggaa ttcctgggcg cctggatacc tgtcatccgg ttccctggcc
     8101 agcgatttac ggataatcga ggcgacgccg tcatcgatct ccgggtgctt ggacaggcgc
     8161 ttgtcggcgt ccgcgtccct gatatttatc aacgtctgca gatccgtctt gccaaggaac
     8221 atccgcttca gggtcaaaat ctcaaacagg atgatgccaa gcgaaaacaa atccgatcgg
     8281 ttgtcgattt cacgcccaag aacggtttcc ggggacatgt acccaagctt gcccttcagg
     8341 acgccggtct gcccctcgtt ctttgaattg gcggccttcg cgacaccgaa atcggtgacc
     8401 ttaacctcgc catcgaacga gatcaggata ttgctggggg atacgtcgcg gtgaattatc
     8461 ttcagcggct tcccgtaagc atcgcgggcg ttgtatgcgt accacagggc cttgcacacc
     8521 tcgccgatga tgaaaagggc aaccccgggc ggaatcgtcg cgccaatctt gttggccctt
     8581 gccatcactg cctgcagatc gacgccattg accagatcca tcgccaggta atactggccg
     8641 tcaacctggt cgaactcgta aatcgaaacg atgttggcgt gctgaagcgg cgcggtgatc
     8701 aaggcttcgt ccttgaacat cttgacgaaa gccggatccc gcgaatatct gacaagaatc
     8761 cttttgatta caagaacttt ctgaaaatcg cccgcaccgc gggaaacggc cttgaatatc
     8821 tcggccatcc cgcccgttcc gatcttctca agcagggtat atttcccgaa ctgccgcgga
     8881 cgcatcctgg cctgttccgg attttgaacg actgcgctgt cctgaacgtc cgccacaaac
     8941 aacccccggg gttcgacgca tcggccgggt cagtcccgac cgccttgaac gccgcttccc
     9001 aacgatttaa taaccgccag cgcgctttgg caatagtgtc tttcagcaag tccggcctga
     9061 tcagcaaatt ccttcatttc ctggcactgg gtcgagcccc cgccacgctt caggcagacg
     9121 tgatccgcca gcctggaaca aggttcggtg catgaaacgc tgacaagggg aagaattgcg
     9181 cacgcaagca acaaaagccg ttttttcata cgactccgga gtccgacaca aaaaccagaa
     9241 gacaccacgg acgatagcaa ctcagaccgg aacgccgcga gagcaactta cttgatgccg
     9301 cccagattga tcagcacgct gccgttccc
//
LOCUS       DAOPPA010000063         9290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8389_length_9290_cov_4.108609, whole genome shotgun sequence.
ACCESSION   DAOPPA010000063 DAOPPA010000000
VERSION     DAOPPA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8389_length_9290_cov_4.108609"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKH54_03205"
     CDS             <1..532
                     /locus_tag="PKH54_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC98924.1"
                     /translation="GSLDVLPSHPDVVAAEVELVDVEDRAGLLKAALGSSDRYDIIII
                     DCPPALGFLTLNALVAASRVVVPMQCEFYALDGLARLVQTIDLTRRSWNPDLMLLGLV
                     LTMFDSRNRLSHQVAEDVVRHFPGQVFNTRIPRNIRLAESPSHGKPVILFDVRATGAA
                     ANMDLAAELLEKLQLN"
     gene            550..1446
                     /locus_tag="PKH54_03210"
     CDS             550..1446
                     /locus_tag="PKH54_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOC98925.1"
                     /translation="MNQKRPALGKGLSALFPASVTTASQPLFECPVDRIDAQDGQPRT
                     RFDDEALRDLAESIKVSGIIQPLVVTDSGNGRYGLIAGERRLRAARMAGLAAVPVIVR
                     QAAPGDVFLMALIENIQREDLNPVEQARAWQRLVREFGMTQEEAASRVGRQRSTVANS
                     LRLLKLSPEVLEALEDGQIVEGSARALIGLDRERQLQLLLQIRDRGLSTREVEALASR
                     QSAARPPRERRQPAMAAYFCGARQEIQEALDLPVTISFRGNRGKISISFNNLAQFRAL
                     RDSLVESSGKSKQDEPSEKKGR"
     gene            complement(1399..2028)
                     /locus_tag="PKH54_03215"
     CDS             complement(1399..2028)
                     /locus_tag="PKH54_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98926.1"
                     /translation="MKGPARYIVAVCLLIPISIAVIRVGVSGSDDLKTALACEKAGLT
                     DTAIDFYGRAARWYLPFSSTQEQALQGLLNISAGSTGPEGDKTAIRALYEARGAILAT
                     RWLMTPSSDMLKEVNDTLARRIARGSKPGEDGADIDGNLAILSRTTSPRPVVSFLATL
                     LFAAWVTVTIVGATRSVTSDGRFAGRRGLPWMAVSAALLFGWLILLALA"
     gene            complement(2025..2834)
                     /locus_tag="PKH54_03220"
     CDS             complement(2025..2834)
                     /locus_tag="PKH54_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC98927.1"
                     /translation="MKNGFADSIRRTFAIARNTVLEAIRNRAFLGMGIAAFGLVVSSI
                     ALSSLAVRDQAERVLVDFGLAATSLMEIVIAITMGVILVFKEIDRKTFYLVLPKPIRR
                     SEVIAGKFVGLLGVLGASLLLMAAGWLISLQVKGISIPAGVPQALLLVWAQAALITSV
                     AIFFSSFASPVLSGVFTFGVFMVGSSVTVLQELMQARKGMLATPGPARLIAEGAVAVF
                     PDLSVFNVGKELILRIPVTWEYVGASMLYCGFYCLFFLAIGALVFQRRDFS"
     gene            complement(2831..3808)
                     /locus_tag="PKH54_03225"
     CDS             complement(2831..3808)
                     /locus_tag="PKH54_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC98928.1"
                     /translation="MDEIIRVENLSKTFRTPFRRKRVDAVSNVSFVVRRGEIFGFLGP
                     NGAGKTTTIKMLTGLIQPTAGTVSMLGGGPADLSVRARIGFLPEQPYFYDYLTPVELL
                     DVFGRIFGIPARIRKERIESLLTQVGLFEARNRTLRKFSKGMLQRTGIAQALLNDPEL
                     VILDEPLSGLDPVGRKEVNDLIATLRQQGKTIFFSSHILADIERLCDRVVILDRGVTK
                     AAGTLSELLTSDSGEREILAGGITDVSGLNGLDGVVRCETYGTDAVRIVCESAASDRL
                     VGTLLKAGGTILGVNDRRISLEQLFVSRTIREKSWPATDNEEEVRGVSE"
     gene            complement(3847..4995)
                     /locus_tag="PKH54_03230"
     CDS             complement(3847..4995)
                     /locus_tag="PKH54_03230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC98929.1"
                     /translation="MADNGGTDRRTGARSPVPLARPCFSQHELGAVRRVMQSCHVTQG
                     PETAAFEADLSALVGGRETVAVSSGGAALLLAMHAIGIGPGDEVVVPDFVFPAAAMGA
                     MFMGAVPVPADVETETMAMTPETAARCITPRTKAVVAVHPFGIPADIEGIVQACPEGV
                     PVIEDAACALGGLTAGGAAAGTVGRISCFSFHPRKSITTGEGGCLAASGSDADRLRRL
                     RDYGRTGRGFGDVFGEIGMNFRMSDLAAAIGRVQLERVHESISTRQKLFSRYATNLGD
                     IAGLRIPGGYLRPGNTFQSLVARVQVDARALVSELAARGVQSGPSAHSLRGQTAFAAR
                     YPGLAGAGDGSGRMLAEHTVVLPLFDRMTFKDVDFVCEAVRDALRHMA"
     gene            complement(4995..5942)
                     /locus_tag="PKH54_03235"
     CDS             complement(4995..5942)
                     /locus_tag="PKH54_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC98930.1"
                     /translation="MRKRILITGGAGFIGSSLALRLKDDFDVVIFDNLHRNAFEKTPL
                     AGAPSVTLVKGDVLDLQAVRQAAKGCSVVFHMASIAGVDTVMKNPVLTMKVALQGTMN
                     VLEAVSGLESFDRFVDLSTSEVFGQYAYKVTEGDITQLGAVGEARWTYAVSKLATEHL
                     ALNYHKQYGLPALSIRPFNIFGPRQVGEGAIHHFIRRALAGEDVVVHNDGSQIRAWCY
                     INDIIDALLLVMERPEAVGEAFNIGNPRSTITIYNLAREVIRIAGSRSRIVQKDWPFA
                     DVELRIPDISKARRLLGFEPSWNLEDGLERTVDWYREFS"
     gene            complement(5939..6955)
                     /locus_tag="PKH54_03240"
     CDS             complement(5939..6955)
                     /locus_tag="PKH54_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98931.1"
                     /translation="MGRPEDTGDHDGSARPHTTRYRTVATAIVFLALAAAGYAGTRIS
                     LVEMKFARMNPVRDASVVNDLVADRLSPEARAAISTMGSEGGAETLYLPPVLVLKVLS
                     AGHQPALADLLFVRAHAWFLSHFFSDRRFVWLSNYYEAITGLDPDNPKIYTWAAQVIK
                     LGQIIDDDIVNKANGFLSDGLERYPLDWQMHLDLGFNLYFEFKGNTPEEKSIARLKAR
                     DHFATAAGLPGAVIDPNFVAELFQRDQEDGMAVAYALQKYYEATEDQRNQLLRRISVL
                     SDALADGIRQEERRWRSNAGYLPVALFAILDRRPDSMWKSIFGHEMPAIAGKGDTPAV
                     GEPD"
     gene            7099..8205
                     /locus_tag="PKH54_03245"
     CDS             7099..8205
                     /locus_tag="PKH54_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98932.1"
                     /translation="MFFALSALLLSAASLGLSVCAVVRWRRAISAVPGDSASALVVTA
                     ATTSARLDRSVLKAVAASAGRGASGLAAGRLPGDSATRVVLEEVNAQFPGFPIYDVPG
                     VPDGRHVVETALSAIALSGSPGDPILALSSGAMPSTPVLCEMSRGVSGDMVFSAFPTR
                     PRAGRGWFSVSSDVSRLTPVTSLLFGPPGVLSGLTLANRAFVVEALADPLTLNRAGLG
                     AALHFKVATDRFGLLPFPVPVRDDISRTHLKILSRLDSRRFALLAASLLAGPLSLMVL
                     FLSVACAGSFSIPAAVALLLCVGARAAAGSTWQRQACGLKSAFLDALVSPVADAPAFF
                     RLLAAISGRQSDCGGVRCQVRAGGLVIPAGPAGG"
     gene            8207..9265
                     /locus_tag="PKH54_03250"
     CDS             8207..9265
                     /locus_tag="PKH54_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOC98933.1"
                     /translation="MNGLIAGIDSGNTKTAFLCVTTRGEVVVRGVTGPSNYQTLGREG
                     CLGRLRSLVQPHASILASESSRVLGWGFGLAGLDRPRDREVLDAITLELVTGVFGHGH
                     CPQRMLQNDAFLSLRAGSDDGVGVAVSAGTSGNCVGMNRRGERLQIGGIAAELGDGGG
                     GFDIAIAGLKAAGRARDGRAPKTLISDLYMSAMGISDIADVVDFMIPGNPGPDIPGEN
                     AAAAGIEQPIPPLIGLLAPLVFQAAAMGDIPARNILETFGRDLGVSARVAAFRLGFRP
                     GEEFPLVLGGSVLQKATEPTYRNAIVGEVCSMFPNASPLTLSDHPVVGAVLMGFDVAG
                     IPVTPAIRSAVSIAASGM"
BASE COUNT         1672 a         2910 c         2875 g         1833 t
ORIGIN      
        1 tgggtcgctt gacgtgctgc cgtcccaccc cgacgttgtc gccgcggagg tcgaactcgt
       61 tgacgtcgag gatcgggccg gtctgctcaa ggcggcgctg ggttcctccg accgctatga
      121 cataattatc atcgactgtc ccccggccct gggcttcctg accctgaacg cgctggtcgc
      181 ggcgtcgagg gtcgtggtgc cgatgcagtg tgagttctat gcactggacg ggcttgcgcg
      241 actggttcag acaatcgatc ttacccgtcg atcgtggaat cccgacctga tgctgctcgg
      301 ccttgtcctg acgatgtttg acagccgcaa caggctcagc caccaggtgg ccgaggacgt
      361 ggtcaggcac tttccgggcc aggtcttcaa cacaagaatt ccccgcaaca tcaggcttgc
      421 ggaaagccct tcacatggaa aaccggttat actcttcgac gtcagggcaa ccggagcggc
      481 agccaacatg gatcttgccg ccgagctgct cgagaagctt caactgaact gagtctttcg
      541 ggggttccga tgaaccagaa gaggccagcc cttggcaagg gcctgtcggc gctgtttccc
      601 gccagcgtga ccacggcgtc acagccgctt ttcgagtgtc ccgtcgatcg aatcgacgcc
      661 caggacggtc agccgcgcac caggttcgac gatgaagccc tgcgcgatct tgccgaatcg
      721 atcaaggttt ccggaatcat ccagccgctc gtcgtcaccg acagcggaaa cggccgttac
      781 ggcctgatcg cgggcgagcg gaggttgcgg gcggccagga tggcgggcct tgcggcggtt
      841 ccggtgattg tccgtcaggc cgcgccgggc gacgtgttcc tgatggcgct gatcgaaaac
      901 atccagcggg aggacttgaa tccggtcgag caggcccgcg cgtggcagcg tcttgtacgg
      961 gaattcggca tgactcagga ggaggccgct tccagggtgg ggcggcagcg atccaccgtg
     1021 gcgaactcgc tgcgcctgct gaagctgtct cccgaagttc ttgaggccct tgaggacggg
     1081 caaattgtgg aagggtcggc gcgcgcgctg attggtcttg atcgcgaaag gcagcttcaa
     1141 ctcctcctgc agattaggga caggggactc agtacccggg aagtcgaggc gcttgccagc
     1201 aggcaatcgg cagcccgccc cccgcgtgaa aggcgccagc ccgcgatggc ggcatacttc
     1261 tgcggggcca ggcaggaaat tcaggaagcc cttgaccttc ccgtcacgat ttcctttcgt
     1321 ggaaatcgtg gtaagatatc gatatcattt aataatcttg cccagttcag ggccttgagg
     1381 gattctctgg ttgaatcttc aggcaagagc aagcaggatg agccatccga aaagaagggc
     1441 cgctgatact gccatccacg gcagtccccg gcggccggca aagcgaccgt ccgacgtcac
     1501 cgagcgcgta gccccgacaa tcgtcaccgt gacccatgcc gcaaaaagaa gcgttgcgag
     1561 aaacgaaacc accggtcggg gtgatgtggt cctggacagg attgccaggt tgccgtcgat
     1621 gtcggcgccg tcttcgccag gtttcgatcc ccgggcgatt cgccgggcca gcgtgtcgtt
     1681 aacctccttc agcatgtcgg aagaaggtgt catcagccat cttgtcgcca ggatggcccc
     1741 ccttgcctcg taaagggccc ttatggccgt tttgtcgcct tccggccccg ttgaccctgc
     1801 cgatatgttc aaaaggcctt gcagggcttg ttcctgtgtt gacgaaaaag gcagatacca
     1861 tcgggcagcc ctgccataga agtcgatggc ggtgtctgtc aggccggcct tttcgcacgc
     1921 cagggcagtc ttgagatcat cgctgccgga aacgcccacc ctgatcacgg caatcgagat
     1981 cgggatcagc aggcacaccg cgacgatgta tcgggccggg cctttcaaga gaagtccctc
     2041 cgctggaaaa ccagggcccc gatggccagg aagaacaggc agtaaaatcc gcaatagagc
     2101 atgctggcgc cgacgtattc ccacgttacc gggatgcgga gtatcagctc cttgccgacg
     2161 ttgaacaccg acagatcagg aaataccgcc accgctccct cggcgatgag cctggccggc
     2221 ccgggggtcg caagcatccc cttccgtgcc tgcatcagtt cctgaagcac cgtgaccgaa
     2281 ctgccgacca tgaacacgcc gaaggtaaag accccggaca gaaccggact ggcgaaagag
     2341 gaaaagaaga tcgcaaccga ggtgatcagt gccgcctgcg cccaaaccag cagaagcgcc
     2401 tgtggaacgc cggccggaat cgaaatgccc tttacctgaa gggagatgag ccatccggcg
     2461 gccatcagca gcagggacgc gcccagcacc cccagcagcc cgacgaattt gccggcgatg
     2521 acctctgaac gcctgatcgg ctttggcagg acaagataga acgtcttgcg gtcgatttcc
     2581 ttgaacacca ggatgacgcc catcgtgatg gcgatcacga tttccatcag agaggtggcc
     2641 gccagtccga aatcaacgag aacgcgctcg gcctgatccc ttacggccag ggacgaaagc
     2701 gcgatcgagg aaaccaccag gccgaaggcc gcgatgccca tgcccaggaa tgcccggttg
     2761 cggatggcct cgagaaccgt gttgcgtgca atcgcaaacg ttctgcgaat ggagtcggca
     2821 aaaccgtttt tcattcggac accccccgga cttcctcttc attatccgtc gccggccacg
     2881 acttctccct gatcgttcgg gaaacgaaaa gctgctccag ggaaatccgg cgatcattga
     2941 cacccaggat cgtgccgccc gccttcagaa gggttccgac aagcctgtcc gaagccgcgc
     3001 tctcgcacac gattctaacg gcgtcagtcc cgtaggtttc gcaccgcacg acaccgtcaa
     3061 gtccgttcaa cccggagacg tcggtgatcc cgccggccag gatttccctt tcgccgctgt
     3121 cagacgtcag aagctccgac agcgtgccag ccgccttcgt gacgccgcga tccagtatga
     3181 cgacgcggtc gcagaggcgc tcgatgtcgg ccaggatatg cgaggaaaag aatatcgtct
     3241 tgccctgctg gcgcaacgtc gcaatcaggt cgttgacctc tttccggccg accggatcga
     3301 ggccggaaag aggttcgtca aggatgacca gttccgggtc gttcagcagc gcctgtgcga
     3361 ttccggtacg ctgcagcatg cctttggaaa acttgcgcag agtccggttc ctggcttcga
     3421 aaagaccgac ctgggtaagc agcgattcga tcctttcctt tcggatcctt gcgggaatcc
     3481 cgaagatccg cccgaaaacg tccagcagtt ccaccggagt aaggtagtca taaaaatacg
     3541 gctgttcggg aaggaatccg atcctggccc gaaccgaaag gtcggcaggt ccgccaccaa
     3601 gcatcgaaac ggttcccgcg gtcggctgaa taaggccggt gagcatcttg atcgtggtcg
     3661 tctttcccgc gccgttcggt cccaggaatc cgaagatctc gccgcgcctg acgacgaaac
     3721 tgacgttgct caccgcatcg acccgcttcc tgcgaaacgg ggttctgaac gtcttgctga
     3781 ggttttctac ccttatgatc tcgtccaagt ctgcctcccg gtggcccgcg gccaccagaa
     3841 acggtatcag gccatgtgcc gcagagcgtc ccggaccgct tcgcacacga aatcaacgtc
     3901 cttgaaagtc atccgatcaa acagcggcaa caccacggta tgttccgcaa gcatccttcc
     3961 ggagccgtca ccggccccgg ccagccccgg gtacctggcc gcgaaggccg tctgcccgcg
     4021 cagcgaatgg gccgagggac cgctttgcac cccccgtgcc gccagttcgc tcaccagggc
     4081 ccgggcatcc acctgaaccc ttgcaaccag cgactggaaa gtattgcctg gccgaaggta
     4141 tccgcccgga atccgaagcc cggcgatatc ccccagattt gttgcgtatc ttgaaaaaag
     4201 cttctgtctg gtcgagatcg attcatgaac acgttcaagt tgaaccctgc cgatggcggc
     4261 cgcaagatcg ctcatgcgga agttcattcc aatctccccg aaaacatccc cgaagccgcg
     4321 cccggttctg ccgtagtcgc gcagcctgcg cagcctgtcc gcgtccgatc ccgatgccgc
     4381 aaggcagcct ccctcgccgg tcgtgatgga cttgcgcggg tggaatgaga agcaggaaat
     4441 ccttccgacc gtaccggcag cggcgccgcc cgcggtaagg ccgccaaggg cgcaggcggc
     4501 gtcctcgatg accggaacgc cttccgggca cgcctggacg atgccttcga tgtccgcggg
     4561 gatcccgaac gggtgaaccg ccacaacggc cttcgttcgt ggcgtgatgc agcgggcggc
     4621 agtttccggg gtcatagcca tcgtttctgt ctcgacatcg gccggaaccg gcaccgcacc
     4681 catgaacatg gcacccatgg ccgcggcggg aaagacgaaa tccggaacga cgacctcgtc
     4741 gcccggaccg atcccgatcg cgtgcatggc cagcagcagc gcggccccgc ccgacgacac
     4801 cgccacggtt tcccttccac ccacaagggc ggacagatcg gcttcgaacg ccgccgtctc
     4861 aggcccctgg gtcacatggc aggactgcat cacgcgcctg acggccccca gttcgtgttg
     4921 cgaaaaacag ggccgcgcaa gcgggacggg gcttctggcc ccggttcgtc ggtcggttcc
     4981 gccgttgtct gccatcagga aaactcccgg taccagtcga cggttctttc caggccgtct
     5041 tcaaggttcc acgaaggttc aaaccccaga agccttcgag ccttcgatat gtccggtatg
     5101 cgcagctcca cgtccgcgaa aggccagtcc ttctggacaa tccttgaccg ggaaccggca
     5161 atccggatta cttccctggc aaggttgtat atcgtgatgg tgcttcgcgg gttgccgatg
     5221 ttgaaggcct ccccgaccgc ttcgggcctt tccatgacga gcagcagggc atcgatgata
     5281 tcgttgatgt aacaccaggc cctgatctgg cttccgtcgt tgtgcaccac cacgtcctcg
     5341 cccgccagcg cccggcggat gaagtggtgg atggcgccct cgccgacctg cctggggccg
     5401 aagatgttga acggtcgtat cgaaagcgcg ggaagcccgt actgcttgtg gtagttaagt
     5461 gccaggtgct cggtggcaag cttgctgaca gcgtatgtcc agcgggcctc gccgacggcg
     5521 ccaagctggg tgatgtcgcc ttcggtgacc ttgtaggcgt actggccgaa cacctcggaa
     5581 gtggaaagat cgacgaagcg gtcgaacgat tccagtccgg acacggcctc gagcacgttc
     5641 atcgttccct gcagggcgac cttcatcgtc agcaccggat ttttcatcac ggtatcgaca
     5701 ccggcgatcg atgccatgtg gaagaccacg ctgcaacctt tcgcggcctg cctgacggcc
     5761 tgcagatcaa gcacgtcgcc cttcacgagc gttaccgagg gggccccggc aagtggcgtc
     5821 ttctcaaacg cgttcctgtg aaggttgtcg aagatgacga cgtcgaagtc gtccttcagt
     5881 cgaagcgcca gcgaagaccc gatgaatccg gcgccaccag tgataagtat tcgttttctc
     5941 aatcaggttc ccccaccgcc ggcgtgtcgc ccttccccgc gatcgcaggc atttcgtgcc
     6001 cgaaaatgga cttccacatc gaatccggcc gcctgtccag tattgcgaaa agcgcgaccg
     6061 gaaggtaacc ggcgtttgat cgccagcgcc tttcctcctg gcggatgccg tcggcaagcg
     6121 cgtcggagag aaccgatatc cgccgcagaa gctggttgcg ctggtcttcc gtggcttcgt
     6181 agtacttctg cagggcgtac gcgaccgcca tcccgtcttc ctggtcgcgc tggaacagtt
     6241 cggcgacgaa gttggggtcg atgacggccc cgggcaggcc tgcggcggtt gcgaagtgat
     6301 cccgggcctt cagccgggca atcgatttct cttcgggcgt attccccttg aattcgaagt
     6361 aaagattgaa tccaagatcc aggtgcatct gccagtcaag cgggtatcgt tcaaggccgt
     6421 ccgacaggaa cccgtttgcc ttgttgacga tgtcgtcatc tatgatttgg cccagtttga
     6481 ttacctgggc ggcccatgtg tagattttcg ggttgtcggg gtcaaggccg gtgatcgctt
     6541 cgtagtagtt gctcagccac acgaatctgc ggtcgctgaa gaaatgggac aggaaccatg
     6601 cgtgggcgcg cacgaaaagg agatcggcca gcgccggctg gtgaccggcc gacagaacct
     6661 tcaacacgag caccggcggc aggtagagag tctcggcgcc cccctcggag cccatcgtag
     6721 aaatggcggc ccgcgcttcc ggcgaaaggc ggtcggcaac cagatcattt acgaccgagg
     6781 cgtccctgac agggttcatc cttgcgaact tcatctcgac cagggaaatc ctggttccgg
     6841 cgtaaccggc cgcggccagc gcaagaaaaa cgatggcagt ggccaccgtt ctgtatcgag
     6901 tcgtgtgcgg gcgcgccgaa ccgtcgtgat cgcctgtatc ttcgggtctg cccatcgaaa
     6961 ccccgtggtc ttgaaccgga cgccgtttaa ccgtcaggaa tgatatcata gcgccagtgt
     7021 ccgttgtgct gtaatgttgc ggccccgggc aggtttgccg gggtgttccg aacttggaaa
     7081 cggctggtga tccctttgtt gttttttgcc ctctcagcct tgctgctttc ggccgcgtcc
     7141 cttgggctgt cggtctgcgc cgtggtgcga tggcgccgcg cgatttcggc ggttccgggg
     7201 gattcggcgt ccgcgcttgt cgttacggcc gcgacaactt ccgcaaggct tgaccgttcg
     7261 gtgctgaagg cggttgccgc ctcggccgga aggggtgcct cggggcttgc cgccggtcgc
     7321 ctgccgggcg actcggcgac cagggtggtt ctggaggaag tcaacgccca gttcccaggg
     7381 tttccgattt acgacgttcc cggggttccg gacggccgcc atgtcgtgga gacggccctt
     7441 tcggcaatcg cgctttccgg gtcgccgggc gatcccattc tcgcgctttc atccggggcg
     7501 atgccgtcaa ctcccgtgct ttgcgagatg tcgcggggtg tttccggcga tatggtcttt
     7561 tcggcctttc cgacaaggcc gcgcgccggc cgcggatggt tttccgtttc gtcggacgtg
     7621 tcccggctca cgcccgtgac aagtcttctt ttcgggcctc ccggggtgct ttccggattg
     7681 actctggcaa accgggcctt cgtcgtcgag gcgctggcgg atccgcttac gctgaatcgc
     7741 gcgggacttg gagcggccct tcatttcaag gtcgcgacgg atcggttcgg gcttcttccg
     7801 tttcccgttc cggtaaggga tgacatctcg cgcacgcacc tgaagatact gtcccggctt
     7861 gattcgcgtc ggtttgccct gcttgccgcc tcgctccttg cgggaccctt gtcgttgatg
     7921 gttcttttcc tgtccgtcgc atgtgcgggc agcttctcga ttccggccgc cgtcgcgctt
     7981 ctgctgtgcg tcggggcacg cgcggccgcc ggatcgacat ggcagcgcca ggcctgtgga
     8041 ttgaaatcgg cattcctgga cgcccttgtt tcgccggtcg ccgacgcgcc ggcatttttc
     8101 cgtctgctcg cggcaatctc tggtcggcag tccgactgcg gcggtgtcag atgccaggtt
     8161 cgcgccggcg ggctcgtgat tccggccgga ccggcggggg gttgaattga acgggctgat
     8221 tgccggcata gattcgggaa ataccaagac cgcgttttta tgtgtcacca cccggggcga
     8281 ggtcgtggtt cggggtgtca ccgggccgtc caactaccag acgcttggcc gtgaaggttg
     8341 cctcggccgc cttcggtcgc ttgtgcagcc gcacgcatct attctggcct cggaatcctc
     8401 gcgcgttctc ggctggggat ttggcctggc cgggctggat cgtcccaggg atcgcgaggt
     8461 gctggacgcg attactctgg aactggtgac cggtgtcttc ggccacgggc actgccccca
     8521 aaggatgttg cagaacgatg ccttcctttc gttgcgggcc ggttcggacg acggcgtcgg
     8581 tgttgcggtg tcggccggga cgtcgggcaa ctgcgtcggc atgaaccggc gtggcgaaag
     8641 gctccagatc ggtggaatcg cggcggagct cggcgacggg ggcggaggct tcgatatcgc
     8701 gatcgctgga ttgaaggctg ccggcagggc aagggacgga agggcgccga aaaccctcat
     8761 ttcggatctt tacatgtcgg ccatgggaat cagcgacatc gccgatgtgg tggacttcat
     8821 gattccgggc aacccaggtc ccgacattcc gggagagaac gcggcggcgg ccggaatcga
     8881 gcagccgatc ccgccgctca taggtcttct tgcccccctc gtgttccagg cggccgccat
     8941 gggtgatatc cccgccagga acatcctcga gaccttcggc cgcgatctcg gcgtgtccgc
     9001 cagggtggcc gcattccgac tcggcttccg acccggcgag gagtttccgc tggtgctcgg
     9061 cggcagcgtg ctgcagaaag ctaccgagcc gacataccgt aacgcaatcg tcggcgaggt
     9121 ttgttccatg ttccccaacg ccagcccgtt gactctttcg gatcatccgg tcgtcggcgc
     9181 cgtcctgatg ggttttgatg tggccggcat tccggtgacg ccggccatcc gttcggccgt
     9241 gtcgattgcg gcgtccggga tgtgaccgcc gccctgcgcc gtggcccggg
//
LOCUS       DAOPPA010000064         9274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8407_length_9274_cov_4.017572, whole genome shotgun sequence.
ACCESSION   DAOPPA010000064 DAOPPA010000000
VERSION     DAOPPA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_9274_cov_4.017572"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKH54_03255"
     CDS             <1..492
                     /locus_tag="PKH54_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98934.1"
                     /translation="PSLLELVLSDRKQPASLTRAFVTIDGRRHPQPALNDVLFANVVP
                     SATSRYVITVAGVSETHKSSGVWISTAAGSTGAMLSAGGSIQDLQDTRLQYLVREPFG
                     CRPDSIRYRLVSGMLGDEGIVLQSRMIRSGVYLDGRRTAVPVDFGSRITVTPDGPPLL
                     LFR"
     gene            521..1873
                     /locus_tag="PKH54_03260"
     CDS             521..1873
                     /locus_tag="PKH54_03260"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98935.1"
                     /translation="MKLCIVGAGSSRLPLMMASVARSGFSFGEVTLFDIRPDRIQALM
                     PVGLELCAFLGASVPFRIASGASDAIEGCQAMILTIRPGFERQRALDERACLDLGVIG
                     QETTGPAGLAFATRTIPAVTGYCELAERLAPGYLPVIFTNPAGMVTQALIDQGHDRAV
                     GICDSATGAARKAAGWAGIPFHDVVFEVGGLNHLSWTRKVTAGGRDILSAALADREFL
                     ERTVPWHAEETAVTGAVPVEYLFYYLRADQALAAMAAEPLTRGEAVLRSNSELLESLP
                     GMGSREKTIHYARYLFSRNDTYMSYALGHRHGTPGPSTMAEALQYLETEIGGYAEVAM
                     ELLASSASTNGRLMALNVRNGGSIPELDDDDVVETDCTVGSAGIIPGRHARMPAADAT
                     LTVRVKEYERLAVQSVTAAMRGENARAVELAIDALTAHPLVPGRQVAANLVGKLGLFR
                     "
     gene            complement(1885..2481)
                     /locus_tag="PKH54_03265"
     CDS             complement(1885..2481)
                     /locus_tag="PKH54_03265"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC98936.1"
                     /translation="METGCPEREEKGEQGSPAVQARLFDNRTIVFGSEVSPKTATALI
                     EQLLAMDAADPAAPITLFLNSPGGEVNSGFAIYDVIRFIRPPVRIVCTGLSASIAAII
                     LLAARPEHRLAMANARLLLHQPLYSGDVIGPASDLEITANEILKSRARINSIIASATG
                     RDPDQVGEDTQRDFWLTAPEAVEYGLVSRIVSSFSELG"
     gene            2696..3094
                     /gene="yajC"
                     /locus_tag="PKH54_03270"
     CDS             2696..3094
                     /gene="yajC"
                     /locus_tag="PKH54_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOC98937.1"
                     /translation="MSFITSVLRVAQTAPVTTPSAPAEPAQAQGGLLGGGQGGQWWQT
                     LIFFGVMILIFWLLIIRPQQKQRKKQEEFLASLKNGDKVVTAAGLIGRIVTMTGDVVT
                     LELARDVRVRIVKNQVVSHFTESAETDKPE"
     gene            3154..5007
                     /gene="secD"
                     /locus_tag="PKH54_03275"
     CDS             3154..5007
                     /gene="secD"
                     /locus_tag="PKH54_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOC98938.1"
                     /translation="MNKWKMWKLLLVAALATLALIYISPTLLGDSNPSWNPLKKSLSF
                     GLDLQGGLELRYTVDYKKAISDNMLRARDVIAGVVTEGLASEKGMDAENLTDQQKAEL
                     AALFTVERYDFDSLQITFTNEDDVDVVTNEMVREARPDLVRQVPSGNAVVLKMSEQRV
                     AQIRDEVVNQTVDVIRQRVEAFGLVEPDVRKNGDTDIDIQLPGVKGTQLTAVRERIGQ
                     TARLAFRIVDRSETAVNFFQNNSAKLVEFQQKFPEKGNLLRLDQDTTTGQFFVAAEAG
                     LTRGKGDALAKNALISFVKTLHVDDDHLVGYELVEDRDGNQVRRRFFRTHYLFSRADV
                     TGDHLTQAMVQFETTGEPSVSIEFDSQGAKAFEQVTAANVGEYMAIMLDEEVNSAPVI
                     KEKIGGGRARISLGGARAANEILADAQSLVTVLTHGAYKAPVHKVHDFEVGPSLGQDT
                     IDSGIVALLVGIISVFVFMVVYYRAGGIIADIGLLLNVLFTLAILVGFNAALTMPGLA
                     GIVLTIGMAVDANVLILERMREEMRLGKGPRAVVEAAYSRAFTAIFDGNITTAIAGVI
                     LLNFSSGPVYGFSVTLLIGIAVTFLTQVFVTRIIYDWYLDKFRPSQISTGI"
     gene            5023..6333
                     /gene="secF"
                     /locus_tag="PKH54_03280"
     CDS             5023..6333
                     /gene="secF"
                     /locus_tag="PKH54_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOC98939.1"
                     /translation="MEFIKPGSEIDFMRLARYFVAGSIVAVVVSLGIIFGVGFDQGID
                     FKGGTKIITEFKADGNIDRDTIKKVVDELVQSNTSIKGSQVEVQDFDVGSGGSSDTVK
                     FQIFTELPSLLSPTDKLALSKKIEMHFGKGTVVESPFEAGDKFYLYLNNEWPRDAAMT
                     EIGKVFAANGHEHVSVISDKEERIRADMLREKDLLLSSSDVDMKAEAEKVETDATRRI
                     AGISDSRFIVEVQAIKDMVSDRMASEFGDKFVSVISTASVSPSVGHELFNTAMLALFY
                     AVIGILIYIAIRFDMKFAPGAILCIVHDVIIVMALMIVFGVKFTLPIVAALLTLIGYD
                     INDTIIVYDRIRENIGKGRGNDLRTHINVALNETLSRTIITSGTTLLTVLSIWLLGGG
                     TTSDFAFVLLTGILIGTYSSIFVAAPLTLVLDKYLRRNRLTAKV"
     gene            6333..8084
                     /gene="recJ"
                     /locus_tag="PKH54_03285"
     CDS             6333..8084
                     /gene="recJ"
                     /locus_tag="PKH54_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOC98940.1"
                     /translation="MPIERETPPIPAMPDEPAISHSWRFFGPDSKAVGRLQSEFGLSP
                     TLASIVLARGLVSDAEISAFLDPSLSRLNNPFLFKDMEAAAARVAQAIAMRESTCIYG
                     DYDADGMTSTALLKLFLAELGLDARTFLPDREADGYGLNTERLAELAASGTRLVICVD
                     CGIKAVDAVEFAGSLGMDLVILDHHMPGTVLPRAVAVVDPHRHDCPFPFKDLAAVGIS
                     FYFCGALRRHMIDTGRLAAPGPDIRQFLDLVAIGTVADVMPLLQDNRILVRAGLERMN
                     GAPRPGIAALRSVSEIRGPITSGSISFRMAPRLNASGRMSHPDTSLSLLVAGDDREAA
                     AWADRLNVDNSLRREVGAQVLEEARRAVLSERTEDSFAIVAAGEGWHPGVLGIVAARL
                     VEEFRVPSIVLSIDGGIATGSGRSVEGFDLGAALAELDRMLLRNGGHPMAAGLSLKMT
                     EFDDFAREFKAIAALRLGGRTQARILDIDAIVTIDVLNNELLAEISSLEPFGIRNREP
                     VLAASGVKIVQARRVGRDGSHLKLQFEQNGTVADGIWFGGADCGLRTGMTVDVAFCLS
                     PDLSGMKPSMKIQDVRI"
     gene            complement(8081..8632)
                     /locus_tag="PKH54_03290"
     CDS             complement(8081..8632)
                     /locus_tag="PKH54_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC98941.1"
                     /translation="MDRDPAIGVLELNSIARGIICTDVMAKAAPVNVALSQTICPGKY
                     LVMITGDEDPVRESMDVGRHYAGNYLVDDILIPNIHEQVVPAVSAVQELAGLDSVGII
                     ETFSVAGTILAADAACKAAGIRLIEVRLANGLGGKAYLTMSGELPDVEAAIAAGVAVL
                     EPAMLVRQEIIPAPHSDMESILT"
     gene            complement(8634..>9274)
                     /locus_tag="PKH54_03295"
     CDS             complement(8634..>9274)
                     /locus_tag="PKH54_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC98942.1"
                     /translation="HHVVRARKVPDRTALRRTSAVCCQCRMCTDMCPRFLLGHRIEPH
                     QAMRGILAGREVSKDVMAMAWLCSQCGVCEEYACDMGLSPKHIFKLMKERLVAEGKKN
                     PCHERPETAMEQNGWIRVPKARLTRRLGLASYLLPHSGEIRVLRDPWLVRIPLKQHAG
                     APSIPVVTVGDAVRAGQLIGEIPEGRLGARIHASISGVVRDVAGGVVTIERS"
BASE COUNT         1824 a         2812 c         2785 g         1853 t
ORIGIN      
        1 ccgtcgctgc tggaacttgt cctttccgac cggaagcagc ccgcaagcct gacccgcgcc
       61 ttcgtcacga tcgacggcag aaggcacccg cagccggccc ttaacgacgt gcttttcgcc
      121 aacgtcgtgc cgtcggcgac atccaggtac gtcatcacgg tggccggggt gtccgaaacc
      181 cacaagagct cgggggtctg gatctcgacg gcggccggct caacaggcgc catgctctct
      241 gcgggcggtt cgattcagga tctgcaggat acccggctgc agtaccttgt ccgggaacca
      301 ttcgggtgcc gtccggactc gatcagatac cggcttgttt ccggaatgct gggggatgaa
      361 ggcatcgtct tgcagtcacg catgattcga tccggggttt accttgacgg ccggcgaacg
      421 gcggttcccg ttgatttcgg cagcagaatc acggttacgc ccgacggccc tccgcttctg
      481 cttttcaggt gatttgcgac cccgtagggt ggagtaccca atgaaactat gtatcgtcgg
      541 tgcgggctca agccgccttc ctctgatgat ggcgtcggtg gccaggtcgg gtttctcttt
      601 cggcgaggtg acacttttcg acatacggcc ggatcgcatc caggcgctga tgccggtcgg
      661 acttgaactg tgtgcgttcc tgggcgccag cgtgccgttc cggatcgcct ccggcgcaag
      721 cgacgcgatt gaaggctgcc aggccatgat ccttacgatt cggcccggct tcgagaggca
      781 gcgggcactt gatgaaaggg cctgccttga ccttggagtc attggccagg aaacgaccgg
      841 gcctgcgggc ctcgcattcg cgaccaggac gatcccggcg gtcacgggtt actgcgaatt
      901 ggccgagagg ctggcccccg ggtaccttcc cgtcatcttc acgaacccgg ccggcatggt
      961 gacccaggca ctgatcgatc agggtcacga cagggccgtc ggcatctgcg attcggcgac
     1021 aggcgcggcg aggaaggccg ctggatgggc ggggatcccg tttcatgacg ttgtcttcga
     1081 agtcggggga ttgaaccatc tgtcctggac caggaaggtt accgccggcg gtcgggacat
     1141 actttcggcg gcgctggccg acagggagtt tcttgaacgg acagtcccct ggcacgcgga
     1201 ggagacagcc gtgacagggg ctgtgccggt cgaatatctt ttctactatc ttcgggcgga
     1261 tcaggccctg gcagccatgg ccgcggaacc gttgaccagg ggcgaggccg ttcttcgaag
     1321 caactccgaa ctgcttgaaa gccttcccgg aatgggatcg cgggaaaaga ccatccacta
     1381 cgccagatat ctcttctccc gcaacgacac gtatatgtcg tatgcgctgg gccacaggca
     1441 cggcacgccg gggccgtcaa ccatggcgga ggcccttcaa taccttgaga cggaaatcgg
     1501 cggatacgcc gaagtcgcga tggaactcct ggcgtcgtcg gcgtccacga acggtcggct
     1561 tatggcgctc aatgtccgga atggcggctc gattccggag ctggacgacg atgacgtggt
     1621 ggaaaccgac tgcaccgttg ggtcggccgg aatcatccct ggacgacacg ccaggatgcc
     1681 ggccgccgac gctacgctga ccgtcagggt caaggaatac gaaaggctgg ccgtccagtc
     1741 ggttaccgcg gcgatgcggg gcgaaaatgc gcgggcggtc gaactggcga tcgatgcgct
     1801 gacggcgcat ccgctcgtcc cgggcaggca ggtcgctgcc aatcttgttg gaaagctggg
     1861 acttttcaga tgaaacaggg cctgtcagcc cagttcggaa aaactcgaca cgattcttga
     1921 aacgagtccg tattccacgg cctccggggc cgtcaaccag aaatcccgct gggtgtcttc
     1981 gccaacctga tcagggtcgc gaccggttgc cgacgcgatt atcgaattga tccgggcgcg
     2041 cgacttcaga atctcgttcg cggttatttc aagatccgat gcgggaccga ttacatcgcc
     2101 actgtaaagc ggctggtgaa gcaaaagcct ggcgttcgcc atggcaagcc tgtgctcggg
     2161 cctggcggca agcagaatga tcgccgctat gctggccgaa agcccggtac acactatcct
     2221 gaccggaggg cggatgaacc ggattacgtc gtagatggcg aaaccggaat tgacctcgcc
     2281 tcccggagag ttgaggaaca gtgtgatcgg tgcggccgga tcggccgcat ccatcgcaag
     2341 caactgctcg atcaaggcgg tcgccgtttt cggagaaacc tccgatccga aaacaatggt
     2401 tctgttgtcg aaaagcctgg cctggacggc cggcgagccc tgctcaccct tttcttccct
     2461 ttccgggcaa ccagtttcca tgctgtctgt aacctcctgg ccgttgcgga tgggaaccga
     2521 aggccatcaa agggcgtccc ggcaaatccc ggaagcgatc cggatcgcgg tcggcacgtg
     2581 ccgcaaaaac tatccgcccg ggcctgggaa gtcaagtcgt gtcgaaagta tatgtgattg
     2641 acgattcaag atgtttctgc tacaagactg cgtccgtttt gtggacggag gtttcatgtc
     2701 attcattacc agtgttttgc gcgttgccca gacggccccg gtaaccaccc cgtcggcacc
     2761 ggccgaaccc gcccaggctc agggtggact tctcggtggt ggacagggcg gccagtggtg
     2821 gcagactctg attttcttcg gcgtgatgat cctgatattc tggctgctga ttatccgccc
     2881 gcagcagaag cagcggaaaa agcaggaaga attccttgcc tcgctgaaga acggtgacaa
     2941 ggtcgtcact gcggcaggtc tcattggaag aatcgtcacc atgaccggtg acgtcgtgac
     3001 tctggaactg gccagggacg tccgcgtccg catcgtcaag aaccaggttg tcagccactt
     3061 cacggaatcg gccgagaccg ataaaccgga gtgatgtctt tgttgatcac ccccggtggc
     3121 ctttccagcc accggtatgc ctgggggtcc tgaatgaaca agtggaaaat gtggaagctg
     3181 ctcctcgtgg cagcactggc aacgctggcg ttgatttaca tatcgccgac gctgctgggc
     3241 gactctaatc cgtcatggaa tcctctgaag aagagcctgt ccttcggtct cgaccttcag
     3301 ggcggccttg aactccgcta caccgtcgat tacaagaagg ccatcagcga caacatgctg
     3361 cgggccaggg acgtgattgc cggggtggtc accgaagggc tggcgtccga aaaaggaatg
     3421 gacgcggaaa acctgacgga tcagcagaag gccgaactcg ccgcgctttt cactgtcgaa
     3481 cgctacgatt tcgattccct tcagatcact ttcacgaatg aagacgacgt cgatgtcgtt
     3541 acgaacgaaa tggtccgcga ggcccgcccc gacctggtca ggcaggttcc gtcgggaaat
     3601 gccgtcgttc tgaagatgtc ggaacagagg gtggcccaga tccgggacga agtcgtcaac
     3661 cagaccgtcg atgtcattcg ccagcgcgtc gaggcattcg gactggtcga gccggacgtc
     3721 cgaaaaaatg gggacacgga tatcgacatc cagctccccg gcgtcaaggg aacccagttg
     3781 accgcggtca gggaacgtat cgggcagacc gcgcgcctgg cgttccgcat cgtcgatcgc
     3841 agcgagacgg ccgttaactt cttccagaac aattcagcga agctggtcga atttcagcag
     3901 aagttccctg agaagggcaa cctgctccga cttgatcagg atacgaccac cggccagttc
     3961 ttcgtggcgg ccgaagccgg cctgacaagg ggcaagggcg acgcgctcgc gaaaaacgcg
     4021 ctcatctcgt tcgtcaagac gcttcacgtt gacgacgatc acctggtcgg ttacgaactg
     4081 gtcgaggatc gcgacggcaa tcaggttcgc aggcgcttct tccgcaccca ttacctgttt
     4141 tcacgggcgg acgtcaccgg cgaccacctg acccaggcca tggttcagtt tgaaacgaca
     4201 ggcgagccgt cggtcagcat cgaattcgac agccaggggg caaaggcgtt cgagcaggtc
     4261 accgcggcca acgtcggcga gtacatggcg atcatgcttg acgaggaagt caattcggca
     4321 ccggtgatca aggaaaagat cggtggcggg cgtgccagga tttcgcttgg cggagcccgg
     4381 gcggcgaacg agatcctcgc cgacgcgcag tctctcgtga ccgttctgac gcacggagcc
     4441 tacaaggccc cggttcacaa ggttcacgac ttcgaggtcg gaccttccct cggtcaggac
     4501 actatcgatt ccggtatcgt ggcgctgctc gtcggcatca tatcggtttt cgtcttcatg
     4561 gttgtttact accgcgccgg tggaatcatc gcggatatcg gactgcttct taacgtcctt
     4621 ttcacgctcg ccattctggt cggcttcaac gccgcgctca cgatgcccgg tcttgccggt
     4681 atcgtgctca ccatcggcat ggcggttgac gcgaacgtcc tgattctgga acgcatgcgc
     4741 gaggaaatgc gcctgggcaa aggcccgcgg gcagtcgtcg aggctgccta cagccgtgcc
     4801 ttcaccgcaa ttttcgacgg caacatcacc acggccattg ccggcgtcat ccttctgaac
     4861 ttctcgagcg gacctgtcta tggattctcg gtcacgctgc tcatcggcat cgctgtcaca
     4921 ttcctgaccc aggtgttcgt gacacgcatt atttacgact ggtacctgga caagttccgc
     4981 cccagtcaga tttcaacggg catctaaggc gggagcaata gcatggaatt tatcaaaccg
     5041 ggttccgaaa tcgatttcat gagacttgcc cgctacttcg tcgcgggatc catcgttgca
     5101 gtggtcgtct ccctcggcat cattttcggt gtcggattcg atcagggcat cgacttcaag
     5161 ggcggaacga agatcatcac cgagttcaag gccgacggca acatcgaccg cgacaccatc
     5221 aagaaggtcg tcgatgaact ggtgcagagc aacaccagca tcaagggcag ccaggtcgag
     5281 gttcaggact tcgacgtcgg ttccggcggc tcttcggata ctgtcaaatt ccagatcttc
     5341 accgaactgc caagcctgct ttctcccacc gacaagctgg cgctgtcgaa gaagattgag
     5401 atgcatttcg gcaagggcac cgttgtcgag tcgcccttcg aggctggaga caagttctac
     5461 ctgtacctga acaacgagtg gccacgcgat gccgccatga ccgagatcgg caaggtcttc
     5521 gcggccaacg gccacgagca cgtttccgta atttccgaca aggaggaacg gatccgcgcc
     5581 gacatgcttc gcgaaaagga tctgcttctt tcgtcttcgg acgtcgacat gaaggccgag
     5641 gccgaaaagg tcgagacgga cgcgacccgc aggattgcgg gcatttcgga ttcgcggttc
     5701 atcgttgaag tccaggccat caaggacatg gtctcggatc gcatggcttc cgagttcggg
     5761 gacaagttcg taagcgtcat atcgaccgca tcggtgagcc cgtccgtcgg acacgaactg
     5821 ttcaacaccg ccatgctggc cctgttctac gccgtcatcg gcatcctcat ctacatcgcg
     5881 atccgcttcg acatgaaatt cgccccgggc gcgatcctgt gcatcgttca cgacgtcatc
     5941 atcgtcatgg ccctgatgat cgtcttcggc gtcaagttca ctctgccgat cgtggcggcg
     6001 ctgctgacac tcatcggtta cgacatcaac gacaccatca tcgtttatga ccgaatccgt
     6061 gaaaacatcg gcaagggtcg cggcaacgac ctcaggacgc atatcaacgt cgcactgaac
     6121 gagaccttgt ccagaaccat tatcacctcg ggaaccaccc tgcttacggt tctttcgatc
     6181 tggcttctgg gcggcggaac gacctccgac ttcgcattcg tgctgctcac gggcatcctg
     6241 ataggaacgt attcatcgat cttcgtcgcg gcaccgctga cgctggttct cgacaagtac
     6301 ctgcgcagga accgcctcac cgcaaaagtc tgatgccaat agagcgggaa acaccaccta
     6361 tcccggccat gccggatgag cccgcaatca gccactcctg gagatttttc gggccggact
     6421 ccaaagccgt cggccgcctc caatcggaat tcgggctttc accgacgctc gcctcaatcg
     6481 tgcttgccag ggggctggtt tccgacgctg aaatttccgc gttcctggat ccttccctgt
     6541 cccgactgaa caacccgttc ctgttcaagg acatggaagc ggccgcggca cgcgtggccc
     6601 aggcgatcgc catgcgcgaa tcgacctgca tatatggcga ctacgacgcc gacggaatga
     6661 cctcgacggc gctccttaag cttttcctgg cggaactggg ccttgatgcc cggacctttc
     6721 ttccggatcg ggaagccgac ggatatggac tgaacacgga acggctggcc gaactcgcgg
     6781 cgtcgggaac caggctcgtc atctgcgtcg attgcggcat caaggcggtc gatgcggttg
     6841 aattcgccgg atccctggga atggatctgg tcatcctgga tcaccacatg ccgggaaccg
     6901 tcctgccccg ggcggttgcc gtcgttgatc ctcatcggca cgactgtccg ttcccgttca
     6961 aggatctcgc cgcggtcggg atatcgttct acttctgcgg ggcgctccgc agacacatga
     7021 tcgacactgg ccggcttgcg gctccggggc ccgacatccg gcagttcctt gatctggtcg
     7081 caatcggaac cgtcgccgac gtcatgccgc ttcttcagga caaccggatt cttgtccggg
     7141 ccggcctgga gcgcatgaac ggggcgccaa gaccgggtat cgcggccctc aggtcggttt
     7201 ccgagatccg gggcccgatc acctccggaa gcatcagctt caggatggcc cctcgactta
     7261 acgcgtccgg gcgcatgagc caccccgaca ccagcctcag cctgctggtc gcgggcgacg
     7321 accgagaggc cgccgcatgg gccgacaggc tcaacgttga caattcactg cgacgtgaag
     7381 tcggcgccca ggttcttgag gaagcccgcc gcgctgtcct ttcggagcga acggaagact
     7441 cttttgccat cgtggctgct ggtgagggat ggcaccccgg cgtgctcggc atcgtcgccg
     7501 cgcggctggt cgaggagttc cgcgtccctt caatcgtcct ttctatagac gggggcatcg
     7561 caaccggttc tggtcgaagc gtcgagggtt tcgacctggg cgcggcactt gcggaactgg
     7621 atcgaatgct gcttcgaaac ggtggacacc cgatggccgc gggactgtcg ctgaagatga
     7681 ccgagttcga tgacttcgcc cgggaattca aggcgatcgc cgctctccgg cttggtgggc
     7741 gcacgcaggc aagaatcctg gatattgacg ccatcgtgac gattgacgtt ctgaataacg
     7801 aacttctggc ggaaatctct tccctggagc cgttcggaat caggaaccgc gaaccggttc
     7861 ttgcggcaag cggcgtgaaa atcgttcagg ccaggcgggt gggtcgcgac ggaagccact
     7921 tgaaactgca gttcgaacag aacggaactg ttgcggacgg aatctggttc ggcggcgcgg
     7981 attgcggact acggacggga atgacggtcg atgttgcctt ctgcctttct ccggatcttt
     8041 ctggaatgaa gccttcgatg aaaattcagg atgtcaggat ctaggtgagg atggactcca
     8101 tgtcggaatg gggtgccggg atgatctcct gccgcacgag catcgccggt tcgagcaccg
     8161 ctacgcccgc ggcgatcgcc gcctcgacgt caggcagttc gccgctcatt gtcaaatacg
     8221 ccttcccgcc aaggccgttc gccagacgaa cctcgatcag ccggatgccg gccgccttgc
     8281 aggcggcgtc ggctgccagg atcgtgccgg cgaccgagaa cgtctcgata attccgaccg
     8341 aatcgagtcc ggcaagttcc tgaaccgccg aaaccgcggg aacaacctgt tcgtggatgt
     8401 tcgggatcag gatgtcgtca accaggtagt tgcccgcgta gtgacgtcca acatccatcg
     8461 actctcgcac gggatcctcg tccccggtaa tcatgacaag gtacttgccg ggacagattg
     8521 tctgcgaaag cgccacattg acgggcgccg ccttggccat gacgtccgtg cagatgattc
     8581 ccctggcgat gctgttgagt tcaagaaccc cgattgccgg atcacgatcc atttcaagac
     8641 ctctcaattg tcacgacgcc gccggcgacg tcccttacga caccacttat cgaagcatgt
     8701 atcctggcgc ccagccgacc ttcgggaatt tccccgatca actggccggc ccttaccgcg
     8761 tcaccgacgg tcaccaccgg gatcgaaggc gccccggcgt gctgcttcaa cggaatcctg
     8821 accagccacg gatcgcgcag aaccctgatc tcgccggaat ggggaagcag atacgaagcg
     8881 agccccaggc gtctggtcag cctggccttc gggacgcgaa tccagccgtt ctgctccatg
     8941 gccgtctcgg gcctttcatg acacggattc ttctttccct cggcgaccag ccgttccttc
     9001 atcagcttga agatgtgctt cggggacagg cccatgtcgc acgcatattc ctcgcacaca
     9061 ccgcactggc tgcaaagcca ggccatggcc atgacgtcct tcgacacctc gcggccggca
     9121 agaatgccgc gcattgcctg gtggggctcg atcctgtgcc ccagcagaaa gcgggggcac
     9181 atgtcggtgc acatcctgca ctggcagcag acggcggaag tgcgacgcag cgcggttctg
     9241 tccggaacct tgcgcgccct gacaacatga tgat
//
LOCUS       DAOPPA010000065         9260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8427_length_9260_cov_3.557849, whole genome shotgun sequence.
ACCESSION   DAOPPA010000065 DAOPPA010000000
VERSION     DAOPPA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8427_length_9260_cov_3.557849"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1528)
                     /locus_tag="PKH54_03300"
     CDS             complement(119..1528)
                     /locus_tag="PKH54_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC98943.1"
                     /translation="MRMKSVELAGRGLQTGIAAFTVALAAMAFSAVEPAAAQEFTLPQ
                     IEAPAPGPVMTLNDAVGLADSGNRSILALRQDIVIASEKLRSSWSGLLPMLYGNMDWA
                     FGTRQTQTGGSSTGHQLGAGLTLTVPLIDAQKWLGMKTAEVESDLVGLQVEESRQILL
                     YSVAEAYYQAATALSLIDVYRAQYDAAAEHMKLAEGRLSAGVGNIMDVRSAQADMIAA
                     WTGMVQAGYSLSDAREALALLIGRDDLPMPVSSPDASGKGLDIDQAVQELAREVELPS
                     FDRDAVDSRWDMKVLAMQKELQRSELKAGYMPFVPRLNGFFNYDVDILGDSSIPNYDR
                     NSWRLGLSLSIPLFDYSIYPGLRQKRAAIRQTELRIDELRAEGRKAVSESARKVVKLG
                     RMMAAARVKAAIADDLLSLAEADYANGTGLAIAVTDARRTSQSAHVELATATWEYQVG
                     LLKLNRELGVDIRTAVTAD"
     gene            complement(1535..1858)
                     /locus_tag="PKH54_03305"
     CDS             complement(1535..1858)
                     /locus_tag="PKH54_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOC98944.1"
                     /translation="MNIMLVSVTERTREIGLRMALGAKPAAILIQFVTEAVVLSVIGG
                     LLGVAAGIGAARYLVTTFDWPLLIEPMFSVIAVGFSGIVGVVFGLYPAWKASRLDPIQ
                     ALRFE"
     gene            complement(1855..2763)
                     /locus_tag="PKH54_03310"
     CDS             complement(1855..2763)
                     /locus_tag="PKH54_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC98945.1"
                     /translation="MTTVQTIWVACRALLRNKMRSFLTTLGIVIGVAAVISMSAIGAG
                     ARARVSSMFESMGANILIVFSGSSRQGGMRGGQGSQPTLTWADLDRIREDIPDVRYAA
                     PMMGIGAQVMAEGQNWQTQVRGTTADYFGIRNWPAASGEIFGDVDEDAGAKVAVVGQT
                     VVNNLFGEDYNPVGQLIRINNIPFSIIGVAAKKGNNAWGSDEDDVVFVPSSTFRAKLQ
                     GGLQQFIPGTIMVGAVSQEATGSAKTAIEKLLRERHRIRPGNEDDFTVRNMSEMASAQ
                     QEGARTMTSLLAGIALVSLIVGASAS"
     gene            complement(2760..3479)
                     /locus_tag="PKH54_03315"
     CDS             complement(2760..3479)
                     /locus_tag="PKH54_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC98946.1"
                     /translation="MDPVIRLEEITRVFDTGDVQVHALRGVSLQVSPGDCLAIMGASG
                     SGKSTLMNILGCLDRPSSGSYHLVGEDVARLSRNQLAQIRNRTIGFVFQNFNLLARTS
                     ALENVELPLLYRGVSARDRRRLAASALERVGLADRMHHGPSQLSGGQQQRVAIARALV
                     GSPSVILADEPTGNLDSKTSVEIMQFFRGLVDDTGVTLVLVTHEPDIAAWADRMVEMK
                     DGLIVRDRLRDQGTSPSEVTA"
     gene            complement(3486..4769)
                     /locus_tag="PKH54_03320"
     CDS             complement(3486..4769)
                     /locus_tag="PKH54_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC98947.1"
                     /translation="MKKGTRFVVIPVVAAVVAAIVILLVFKGRDDTAGKPVWQTTKVH
                     RGDVAYKVTASGTLSPLVTVNVGSQVSGRLQEILVDFNSPVKAGQLIAVIDPSITELE
                     VQRARASVQTARAGIAKASAVVSDRKQVSDRTSVLRDRRLATDMEADSAAAALESARA
                     ELLSARAQLAQAQAMLKQAQTNLGYTKIVSPIDGVVISRQIDVGQTVAASFQAPVLFQ
                     IAGDLRKMEVHTSLAESDVGQVHDGMPVEFTVDAFPSRVFKGEVKQVRYSPTTVQNVV
                     TYDAVVSVDNEDLQLRPGMTADVSFIVSKSTNALVVSNTALRFKPPSVAEPDPKSPPR
                     QSGGSLFGFMPRPMQQKPSETDETEMAKKTAGRRTVWVPGPAGDAVQVAIEAGITDGR
                     ITEVLSGLKEDDLVITGVESGASGAAKGQPGSGRM"
     gene            5061..5453
                     /locus_tag="PKH54_03325"
     CDS             5061..5453
                     /locus_tag="PKH54_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98948.1"
                     /translation="MAIVKSKRVLALVTATVFMAGTVSVCLHGPDDQHASCGGHDHFH
                     MTGQDSPGDDHHDVGAHGPSVSCDSHFPSGDNHSDIHMCPCVSHIQCDSIAGNPVPTG
                     QIVSMRPLEDPVPMPSGFSDLPYRPPVC"
     gene            5515..6765
                     /locus_tag="PKH54_03330"
     CDS             5515..6765
                     /locus_tag="PKH54_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC98949.1"
                     /translation="MKGAIMKLSYASCAAIILALAAPAQARGREAGPVTLTWPDILAA
                     IDGHPAIALASARTGHAMAGATESPVGNPEISGSVGMGVSADGQERAVIWGIDVGIAL
                     DEIPSRIAMRRAALADAEAEQAKAAQIRSEVVAMLHEAFINASIRREIEVASREAAAA
                     ATELAEVAKSRHQAGEGRKIDVMRLEYDAEISKGRLSGAVLERQAAFGRLNRLLGSRL
                     PESFDIDCDWAALPEIAGLDDLLAAGIDSNPAVRAANADARAGHAVLSAERARLVPGF
                     RIGGFYERELDADKFGASLSMTIPLWDHNRKGINQAAARVAGARAEADLARLDYSDRL
                     ASAHARASISRETAIRYRDVVLPLAEQASAMAVALYKAGENGLVETIDARAACAQARM
                     QMLDAFLEGWRAAIELQAITGVTP"
     gene            6762..8213
                     /locus_tag="PKH54_03335"
     CDS             6762..8213
                     /locus_tag="PKH54_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC98950.1"
                     /translation="MTRIYPAAIFLATAIFVISGCDRDGSPQGRGVATEKAADRHGHP
                     ADEPAGHDHPAHAHEHDEHGAASDHPANADEHDEHATGHDHPTHAHEHDGPGDLDRSI
                     PELLEMDCEHGIKAVNCDECRYESGFVKVDPDLFEKGLMKTVTAEETTIASGMTLYGE
                     TRLSERRIARLSPRTSGQVASVGIAPGDHVRRGQVLVEIESTAFAGAAAEWLAAEATF
                     RLADISARRHQDLNRQGLNSTREFQEAQSAVEAARIRLDAAALGLKAAGMTRADARAL
                     SSGARNRVAIRAPFDGEVFEVNATLGAFIEPGQETVVMGDRGTLEVMVDLRESDLEAV
                     SAAFGPSGIPASVTVAAWPGRSFSGILDLIEPTMSRQTRTVRARVTVTNDGLLLRPGM
                     FATVRPQIRGDGVLAITVPAEAVLTDEGRQFVFVHHDGPLFMRRPVKAGKPENGIVEV
                     TSGLAPGQTVVTTGAFLLKSDVLKAKMGEGCAH"
     gene            8229..>9260
                     /locus_tag="PKH54_03340"
     CDS             8229..>9260
                     /locus_tag="PKH54_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC98951.1"
                     /translation="MIDFIVKNRTAVILATALMSAAGLFAWHRMPIDAFPDATNQQVM
                     ILTAADGLGPVDVERQVTIPVESAMGGLPHVTMVRSTSKTSLSQVVVIFEDHVGTWFA
                     RQLVSERLSMIAGTLPAGAIPKMGPATTGLGEVFQYTLESPERTLTELRTMQDRIVAP
                     RLRSISGVNEVNSFGGKVRQVEVLLDPNRLAAFGIAPTDVVEALAADNANVTGGFIVR
                     GWEQENVRTVGLFGDIEEIGKVVLKSENGIPVTIDQVAAIVEGSLPRFGAVTRDGRGE
                     AVAGMVIMLQGENAAEVVGRVRAALPSIRQALPADVEINVYYDRVELVDAVIGTIQGA
                     LLQGGLLVIL"
BASE COUNT         1845 a         2909 c         2806 g         1700 t
ORIGIN      
        1 cccggtcgcc gacaagcagc cgctgtcaat caccaacccg gtgttcattg atgcggatgg
       61 aaacggttac gaggcgattt acaagaacgg cgccccctgg gatgccgagt gacaacgttc
      121 agtccgcggt gaccgccgtc ctgatatcca ccccgagttc ccggttcagc ttcagaagac
      181 cgacctgata ctcccaggtg gcggtggcca gttccacgtg ggcggactgc gatgtgcgac
      241 gcgcgtcggt gaccgcgatg gcaagcccgg ttccgttcgc gtagtcggcc tcggcaagcg
      301 acagcaggtc atcggcgatg gcggccttca cgcgggctgc agccatcatc cgccccagct
      361 tcacgacctt ccttgcggac tccgacacgg ccttccgacc ttcggccctc aattcgtcga
      421 tgcgcagttc ggtctgcctg atcgcggcac gcttctggcg aagtcccggg taaatcgaat
      481 agtcgaacaa cgggatggac aacgaaagtc caagacgcca cgagttgcgg tcatagttcg
      541 gaatggacga atcgcccagg atatcgacgt cgtaattgaa aaagccgttc aaacgcggaa
      601 caaacggcat gtatcccgcc ttcaactcgg atcgctgcaa ctccttctgc atggccagga
      661 ctttcatatc ccatcgcgaa tcgaccgcat cgcgatcaaa agacggaagc tcgacttccc
      721 gcgccagttc ctgaaccgcc tggtcgatat ccagcccctt tcccgatgca tccggacttg
      781 agaccggcat gggaaggtcg tcgcgaccga taagcagcgc cagggcctcg cgggcgtccg
      841 acagggagta tccggcctgg accattcccg tccatgctgc aatcatgtcc gcctgggcgc
      901 tgcgcacgtc cattatgttt ccgaccccgg ccgaaagacg cccctcggcc agcttcatat
      961 gttccgcggc ggcatcgtac tgtgcgcgat agacatcgat aagggaaagg gccgttgcgg
     1021 cctggtagta tgcctcggcc accgaataca gcaggatctg cctggactcc tcaacctgaa
     1081 ggccgaccag gtcggattcg acttccgcgg tcttcatccc gagccatttc tgggcgtcga
     1141 taagcggaac ggtcagcgtc aggccggcac caagctgatg cccggtcgaa ctgccgccgg
     1201 tctgcgtctg cctggtaccg aaggcccaat ccatgttccc gtacagcatg ggaagaagac
     1261 cggaccagct tgatcgcaac ttttcggatg cgatgacaat gtcctggcgc agcgccagaa
     1321 tcgaacggtt gcctgaatcg gccaacccca cggcgtcgtt caaggtcatg accggccccg
     1381 gggcgggagc ctcgatctgc ggcagagtga attcctgcgc ggccgccggt tcaacggccg
     1441 aaaaagccat tgccgccaac gccaccgtaa acgccgcgat cccggtctgc aggccgcggc
     1501 ccgcaagctc aactgatttc atcctcatga agcctcactc gaatctcaga gcctgaatcg
     1561 gatcaaggcg cgacgccttc catgccgggt aaaggccgaa aaccactccg acgataccgc
     1621 tgaacccgac cgcaatcacg gaaaacatcg gttcaatcaa taatggccag tcaaacgtgg
     1681 tcaccaggta cctggccgcg ccaattccgg cggcgacacc aagcagtccg ccgatgactg
     1741 acagcacgac ggcttcggtc acgaactgaa tcaaaatggc cgccggcttg gcgccgagag
     1801 ccattcgaag gccgatttcc ctggttcgct cggtcacgct gacgagcatg atgttcatga
     1861 tgccgatgcc ccgacaatca ggctgaccag cgcaattccg gccagcagcg acgtcatcgt
     1921 ccgcgcgcct tcctgctgcg ccgatgccat ctcggacata ttccggactg tgaagtcgtc
     1981 ctcgttgccg ggccgaatcc tgtgacgttc acgaagaagc ttctcgatcg cggtcttggc
     2041 gcttccggtt gcctcctgcg acacggcccc gaccatgatt gtcccgggga tgaactgctg
     2101 cagtccgccc tgcaatttcg ccctgaaggt cgatgacggc acgaaaacga catcgtcctc
     2161 gtcgctgccc cacgcgttgt tacccttctt cgcggcgacc ccgattatgg aaaacggtat
     2221 gttgttgatt cgaatcaact ggccgaccgg gttgtagtcc tcgccgaaaa gattgttcac
     2281 cacggtctgg ccgacgacgg ccaccttggc gccggcgtcc tcatcgacat caccgaagat
     2341 ttcaccgctg gcggcgggcc agttccgaat gccgaagtaa tcggcggtgg ttccccttac
     2401 ctgcgtctgc cagttctggc cctcggccat gacctgggca ccgattccca tcatcggtgc
     2461 cgcataccgg acatcgggaa tgtcttcacg gattctgtca agatcggccc aggtcagtgt
     2521 cggctgggat ccctggccgc cgcgcatgcc tccctgccgg gaagatccgg aaaagactat
     2581 caggatgttg gcacccatgc tctcgaacat ggaagagaca cgggcgcgcg cgccggcgcc
     2641 aatcgccgac atcgagatca cggccgccac gccgattaca atgccaagcg tcgtcaggaa
     2701 gctgcgcatc ttgttccgca gcagggcgcg gcaagccacc cagattgtct ggacggttgt
     2761 catgcggtga cctccgaagg cgacgtgccc tggtcgcgta acctgtcacg aactatcagc
     2821 ccgtccttca tttcaaccat cctgtcggcc caggccgcga tgtcgggctc atgggtaacc
     2881 agtacgagcg taacgccggt gtcatccaca aggccccgga agaactgcat gatttcaaca
     2941 ctggttttgg aatccaggtt tccggtcggc tcgtccgcca gtatcaccga cggcgatccg
     3001 accagcgccc gggctatcgc gacccgctgc tgctgaccgc cggaaagctg gctcgggcca
     3061 tggtgcatcc tgtcggcaag accgacccgc tccagggcag aggcggccaa ccgacgccgg
     3121 tcgcgcgcgg aaacacccct gtaaagcaac ggaagctcga cattttcaag tgccgaggtc
     3181 cgggccagca ggttgaaatt ctggaatacg aaaccgatcg tcctattacg gatctgtgcg
     3241 agctggttcc tggaaagcct ggcgacatcc tcgcccacca ggtggtacga accggaggac
     3301 ggccgatcca ggcagccgag aatgttcatc aatgtcgatt tgccggatcc ggatgcgccc
     3361 atgatggcaa gacagtcgcc gggcgacacc tgaagggata ccccgcgcag ggcgtgtacc
     3421 tgcacgtcgc ccgtgtcgaa taccctggtg atttcttcaa gcctgattac cggatccatg
     3481 aacggtcaca tcctcccgct gcccggctgg ccttttgccg caccggatgc tccgctttca
     3541 acgccggtga tcacaaggtc gtcctctttc aacccggaaa gcacttcggt aatcctgcca
     3601 tcggttattc cggcctcgat cgcgacctgg acggcatcgc ccgcggggcc gggaacccat
     3661 acggtccgcc ggccggcggt ctttttcgcc atttcggtct cgtcggtttc cgacggcttc
     3721 tgctgcatcg gccttggcat gaatccaaac aggctgccac ctgactgcct gggcggggat
     3781 ttgggatcgg gctcggcgac cgacggcggc ttgaagcgca aggcggtatt cgacaccaca
     3841 agggcatttg tgcttttcga aacgatgaag ctgacatcgg ccgtcatgcc gggtcgaagt
     3901 tgcagatcct cgttgtcaac cgaaacgacc gcatcatacg tcacaacgtt ctgaaccgtt
     3961 gtcggggagt acctgacctg cttcacttcc cccttgaata ccctggaagg aaacgcatcc
     4021 acggtaaact cgaccggcat gccgtcatgc acctgtccta catcggattc cgccaggctc
     4081 gtgtgaacct ccatctttcg aaggtcaccc gcaatctgga aaaggaccgg ggcctggaag
     4141 gacgccgcca ccgtctgccc cacgtcaatc tgccgggaaa tcaccacccc atcaatcggg
     4201 gaaacaatct tcgtgtagcc caggttcgtt tgggcctgct tcaacatggc ctgggcctgg
     4261 gcaagctgtg cccttgctga aagaagttcg gccctggcgc tttccagcgc agccgccgcg
     4321 gaatccgcct ccatgtcggt tgccaggcgc cggtcacgca gcacggacgt acgatcgctt
     4381 acctgcttcc tgtcggaaac gaccgccgaa gccttcgcga tgccggcacg cgccgtctga
     4441 accgacgccc tggcccgctg aacctcgagt tcggtgatgc ttggatcgat taccgcaatc
     4501 aactgaccgg ccttgaccgg ggaattgaag tcaaccagga tttcctgcag ccggccggag
     4561 acctggctgc cgacattcac ggtgaccagt ggtgaaagcg tcccggaggc ggtgaccttg
     4621 taagcgacat cgccacgatg aaccttcgtg gtctgccaga caggcttccc ggccgtatca
     4681 tccctgccct tgaaaaccag caggatcaca atcgcggcaa ccaccgctgc cactaccggg
     4741 atcacgacga atcttgtgcc cttcttcatg tgcaagcccc ttgtgcagcc gccggagcga
     4801 cagccggccc gatcaacgca ccggcgtcac aactgtgacc agcgaatgta aagggaatgt
     4861 tattccccgg acgtcataat gtatcggtaa ttcttcacca ggtacaaggt ggaacgcctg
     4921 tgcccgacct gagtcatccc ctcataggaa agggagtacg ccccgtgatg gttggcggtc
     4981 ggcggtaccc gacagtttga catcggattc gacaatctgc atattgtccg gtgacccggc
     5041 cgcgctgaaa ggtggctctg gtggcgatag taaaatcaaa acgcgtgctc gcgcttgtca
     5101 ccgcgacggt attcatggcc ggaacggtca gcgtctgcct gcacgggccg gacgatcagc
     5161 acgcttcgtg cggcggccac gatcatttcc acatgactgg acaggattcg ccgggcgacg
     5221 atcatcacga tgttggggcg catggcccgt cggtttcatg cgacagccat ttcccgtccg
     5281 gtgacaatca ttcggatatc cacatgtgtc cgtgcgtcag tcacattcag tgcgactcga
     5341 tcgcgggcaa tcccgttccg accggccaga tcgtctcgat gcgtccattg gaggatcctg
     5401 ttccgatgcc cagcggattc agcgatcttc cttaccgtcc ccccgtctgc tgattccatc
     5461 gacaaccgaa ccccgtgact gaatgacgtc ggcaccgcgc ctggcgcgac cggcatgaaa
     5521 ggagccatca tgaagctgtc atacgcttcc tgtgccgcaa tcatcctggc actggccgca
     5581 ccggcgcagg cacgtggccg ggaggccggg ccggtgacgt tgacatggcc ggacatactt
     5641 gccgcaatcg acggtcaccc ggccatcgcg ctggccagcg cacgaaccgg gcacgccatg
     5701 gcgggtgcaa ccgaatcccc ggtgggaaac cctgaaatat ccggaagcgt cggcatgggg
     5761 gtctcggccg acggacagga aagggccgtg atatggggca ttgacgtggg aatcgccctg
     5821 gacgaaatac cgtcaaggat tgcgatgcga agggctgccc tggcggacgc agaggcggaa
     5881 caggcgaagg cggcgcagat acgaagcgaa gtcgtcgcga tgcttcacga agccttcatc
     5941 aacgcgtcca tccgacgaga aattgaagtc gcgtcacgcg aggcggccgc ggcggcaacg
     6001 gaactggcgg aagtcgcgaa atcccgccac caggccgggg aaggccgcaa gatcgatgtc
     6061 atgcggcttg agtacgatgc cgaaatttcc aaaggaaggc tttccggggc cgttctcgag
     6121 cgtcaggctg cattcgggcg cctgaaccgc cttcttggtt cgcggctgcc ggaatccttt
     6181 gatatcgact gcgactgggc ggccctgccg gaaatcgccg gcctcgatga tctcctggct
     6241 gccggtatcg attcaaaccc tgcggttcgc gcggcaaacg ccgacgcaag ggccggacac
     6301 gccgtccttt cggccgaacg ggcaagactg gttcccggct tccgaatcgg cggcttttat
     6361 gaaagggaac tggatgccga caaattcggt gcaagcctgt cgatgaccat tcccctgtgg
     6421 gatcacaatc gaaagggaat caaccaggcc gccgcaagag ttgccggagc cagggccgag
     6481 gcggatcttg caaggctgga ttattccgac aggctcgcgt cggcgcacgc acgggcatca
     6541 atttcgcggg aaaccgccat caggtaccgc gatgtcgtcc ttccgttggc cgagcaggcc
     6601 tcagccatgg cagttgccct ttacaaggcc ggcgagaatg gactggtcga aacaatcgac
     6661 gcgcgtgccg cctgcgccca ggcccggatg cagatgcttg atgccttcct cgagggatgg
     6721 cgcgccgcaa tcgaattgca ggcgataacg ggggttacac catgacaagg atatatcctg
     6781 ccgcaatctt cctggcgacc gccattttcg tgatttccgg ctgcgaccgg gacggaagtc
     6841 cccaggggcg cggcgtcgcg acggaaaagg ccgccgatcg gcatgggcat cccgcggatg
     6901 agccggcggg ccacgaccat ccggcccatg cacatgaaca cgacgaacac ggcgcggcca
     6961 gcgaccatcc ggccaatgcc gatgaacacg acgaacacgc gacgggccac gaccatccga
     7021 cccatgcaca tgaacacgac gggcccggcg atcttgatcg gtcgattccg gagcttcttg
     7081 aaatggactg cgaacacgga attaaggcgg tcaactgcga tgaatgccgg tacgagtccg
     7141 ggttcgtcaa ggtcgatccg gatctgtttg aaaagggctt gatgaagacc gttacggcag
     7201 aagagaccac gatcgcttcg ggaatgaccc tttacggaga gacgcgcctt tccgaacgca
     7261 gaatcgccag actcagcccc cggacgtcag gccaggtggc atccgtcggg attgcacccg
     7321 gcgatcatgt ccgccgcggc caggtacttg tcgaaattga aagcaccgca tttgccggcg
     7381 ccgcagcgga atggcttgcc gccgaggcga cattcaggct ggcagacatt tctgccaggc
     7441 gtcaccagga tctgaaccgc caggggctga actcgacacg ggaattccag gaggcccagt
     7501 ctgccgtcga ggcggccagg attcgccttg acgcggcggc tctgggcctg aaggcggcgg
     7561 gaatgaccag ggcggatgca agggcgctgt cctcgggtgc caggaaccgg gttgccatca
     7621 gggctccgtt tgacggtgaa gtgttcgaag tcaacgcaac cctgggcgcg ttcatcgagc
     7681 cgggacagga aacggtcgtc atgggcgatc gcggcaccct tgaagtgatg gtcgatcttc
     7741 gcgaaagcga cctggaagcg gtttcggccg ccttcggccc gtcggggata ccggcatcgg
     7801 tgacggtggc ggcatggcct ggacgcagtt tttccgggat cctggatctg atcgagccga
     7861 ctatgtcccg ccagacaagg acggtccgcg caagggtaac cgtcacgaac gacgggctcc
     7921 tgcttcgtcc gggcatgttc gcgacggtcc gtccgcaaat caggggtgac ggggtgctcg
     7981 cgatcaccgt gccggcggaa gcggtcctga cggacgaggg ccgtcagttc gtgttcgtgc
     8041 accacgatgg gccgcttttc atgcgccggc ccgttaaggc cggaaagccg gagaacggga
     8101 ttgtcgaagt gacgtccgga ctggctccgg gacagaccgt ggtgaccacc ggtgccttcc
     8161 tgctgaagtc ggatgtcctg aaggcaaaga tgggcgaagg atgcgcgcac tagccgcggg
     8221 ggtgaccaat gattgacttc atcgtgaaaa acaggacggc ggtcattctg gcaaccgcac
     8281 tcatgtccgc ggccggcctg ttcgcctggc accggatgcc gattgatgcc tttcccgacg
     8341 ccacaaacca gcaggtaatg atccttacgg cggccgacgg ccttggtccg gtcgatgtcg
     8401 agcgacaggt cacgattccg gtggaatcgg ccatgggcgg actgccacac gtgaccatgg
     8461 tcagatcgac atcgaagacg tctctttcgc aagtggtcgt catctttgaa gaccacgtcg
     8521 gcacatggtt cgcgcgccag cttgtgtcgg agcgcctctc gatgatcgcc ggaacgcttc
     8581 cggccggcgc aatcccgaaa atggggccgg caaccaccgg cttgggggag gtgttccaat
     8641 atacgcttga aagcccggaa cggacgttga ccgaactgcg cacgatgcag gatcgcatcg
     8701 tcgcgccccg cctgagatcc ataagcggag tcaacgaagt caacagcttc ggcggaaagg
     8761 tccggcaggt cgaggtcctg ctggatccca acaggcttgc cgccttcggc atcgcgccga
     8821 ccgacgtggt tgaagcgctg gcggccgaca acgccaacgt gacgggtggt tttatcgtgc
     8881 gcggctggga acaggagaac gtcagaacgg tcggcctctt cggcgacatc gaggaaatcg
     8941 ggaaggtcgt ccttaagtcc gagaacggga ttcccgtgac gatcgaccag gtcgccgcca
     9001 tcgtcgaggg ctccctgccg cgcttcggcg cggtgacccg cgacggcagg ggcgaggccg
     9061 ttgccggaat ggtcatcatg ctgcagggcg aaaacgcggc tgaagtggtc ggcagggtca
     9121 gggctgctct gccgtccata cggcaggcat tgcccgcgga cgttgaaatc aacgtctatt
     9181 acgaccgcgt cgaacttgtc gatgcggtca tcggcacaat ccagggcgcg ctcctgcagg
     9241 gcgggctgct ggtcattctg
//
LOCUS       DAOPPA010000066         9145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8525_length_9145_cov_4.051485, whole genome shotgun sequence.
ACCESSION   DAOPPA010000066 DAOPPA010000000
VERSION     DAOPPA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_9145_cov_4.051485"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PKH54_03345"
     CDS             complement(<1..1073)
                     /locus_tag="PKH54_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOC98952.1"
                     /translation="MTEILCKTDSFARSVRATVIECVATAPDRFHVVLDRTVVYPGGG
                     GQPADCGTVSGYPILDVRMRPDGGVEHVLQAPVAGVVDVEVNWAARLDFMQQHTAQHL
                     MTAIATARFGWKTIAFHMNPDRSDIEFELDSVSRQDLMTLEEAVNQAVFDDLPVSISF
                     VSREQMSSMNVRSRLLPDGLEGPFRLVGIEGIDLNTCGGTHVQSTGQIQVVKILGTEK
                     LARGVRVFYAAGSRARRIFEQTIERESAIGAALTTGPSDFIASIEALKKSGRDLDTSL
                     RQLSARLAGLVVERMLEDAGSGQVAVYHQDEADGDFLRQVAERFHEARPEALALLTGG
                     ASEGLFMVVGSDESVRRVGPAVAG"
     gene            complement(1092..2405)
                     /gene="ftsY"
                     /locus_tag="PKH54_03350"
     CDS             complement(1092..2405)
                     /gene="ftsY"
                     /locus_tag="PKH54_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOC98953.1"
                     /translation="MESQSFDPSIIVLIVLGIGFVALVVAVVAKMAGKKKGRSLSPSA
                     SARTRDSDDEDDRFELVDQEMTIKRGGRGDRSRTPESSASAAVGQAQRGSRHQDRAGS
                     ASADTEKQVPALKGAADSVAAQSRFRVDISSARRLQEGLSRTRSEGFIARLSEAFGRS
                     SSVDEATIAAAEEALLTADMGVATAMELSEGLRRDFQPSGGPLAPQVMAWLKDRTYDM
                     IRTQPHGMPGLDRPGLNVVMVVGVNGTGKTTNIAKLANWYRNRGRKVLLAAGDTYRAA
                     GADQLGIWAERLGIDVVVGRPGQDPSSLFFDAARKATEEGFDIMIADTAGRLHTDTNL
                     VDELKKVARVLGKAVPDAPHQVLLMLDATMGQNAVRQADVFLKSVNVSGIGLSKLDGT
                     ARGGVVVSIARDMNLPIWFVGVGEAVDDLRSFQPEDFVNALFAAD"
     gene            complement(2460..3650)
                     /locus_tag="PKH54_03355"
     CDS             complement(2460..3650)
                     /locus_tag="PKH54_03355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOC98954.1"
                     /translation="MSNPSVSVARGRARPFWFGCPVVFSGSVSRVHGEPVPGDFVDVL
                     DDMGQLIGRGFFNPGSSYRVRIVARPGEPDTAAAIIARRIADAFRVRAAFGIPSDRTD
                     VYRLFNSEGDGLSGMTIDVFSRTVVVMESALWVSRFRRLIVECLEAGLGPGISVLFRV
                     VGGIRGLEGIMAEDEGTAPVEPIKVVENGLGFLTRPGAGQKTGFYADQRENRLAIRDL
                     CVDADVLDLFCFSGGFALNAASAGAKKITAVDSSADAIAAAAANAAINGFDDRISFVQ
                     QDALKFLAGAREHDVVVCDPPKLASGRSSMESGLKHYARLNVAAMKALRDGGILVTCS
                     CSSVVRRDMFLEMLRDAAVSAGRVISVLDVRGAGRDHPINPSWTEGEYLKVATVVVRD
                     PSRA"
     gene            3717..4334
                     /locus_tag="PKH54_03360"
     CDS             3717..4334
                     /locus_tag="PKH54_03360"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC98955.1"
                     /translation="MKRAITLPSIILIALLGLGAGCHAGTSGTRVAASSGHGKRHVPT
                     ASLNSKRPTVKNSGASRTGSATSAATERCRELLPLITRVASEFDIETSLLVGIVRVES
                     NFRNDVKSPVGAVGLTQCMPATARAKRCGPLNDPYENLRCGARVIKAFLKYYKDSIYL
                     GLSGYNAGHGMPGRARQNLELPQNVDYVEKVLQASARFEERGCDF"
     gene            complement(4350..6503)
                     /locus_tag="PKH54_03365"
     CDS             complement(4350..6503)
                     /locus_tag="PKH54_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98956.1"
                     /translation="MRKLLFASMIGFGMLVACGPGESGTDEGTGVDVGYEDVNQPVDN
                     GQDVQPDTARPDLNQPDDGPVDVGQDHGIDTNPGDSVVLPDNGDCLAGDPCDDGNPCT
                     WGETCGESGECGGGTPYTCDDGRACTDDTCDGLGNCLFVMKAGTCLINGICRDKDAAD
                     PEDPCFVCKPGTSVSEWSKAPDETACDPTVTLGRCEDSPGGTCVDGVCVASTIVPKGC
                     DDSDPCTEDSCNPAVGCINTPVSSGECVLEDKCKPGYCFQGVCIVPEDSSCDDGNSCT
                     LDSCDDVKGCQHSPIEEAACDDGDECTFQDSCFEGVCNGRPQNCDDGNVCTEDGCDSI
                     TGCWHDLATNPCCIGGVSVCDDNNPCTNDDCDPDTFECFHNDNTAACSDGNPCTVGDS
                     CDEGDCFGSAKNCSDGNQCTSDYCDGGLCLHDILSGTACDDGRDCSVNDHCEAGLCVA
                     DESGCVCQPTFYPAVSKFVTMAISTNGKTGSGLDLDMKPTTCAPAEDCCCGIDNALGP
                     VAGMKAAQDGIKDAMAEGSITLLFEHREIKTDGTPYTLAFYSGELAPTNESCNFLTQD
                     CDYVVEPMMITDECLPLVTLNNAKIVGNKLTAGGLDYVFPFDLPLLEGINLHVDLYFT
                     RVEANVTVSGGRVTSMQGILGGAIPKQQISDAVQAVPDETWADLPVDKATVMMLLDLV
                     VIPDIDGDKDGIMESASIGIQFTAIPGEIVGRKVD"
     gene            complement(6541..>9145)
                     /locus_tag="PKH54_03370"
     CDS             complement(6541..>9145)
                     /locus_tag="PKH54_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98957.1"
                     /translation="DDGNPCTTDVCDNGTCLNNATDGAACDDGNRCTFDDICTMNVCG
                     GTAVECDDGNLCTDDSCDPEFGCMNINNEAGCDDGNLCTVGDTCFLGECASGTEPLFC
                     DDSNICTDDGCVPSKGCVAIPNTSPCDDGDPCTVNDFCQASECLAGKLPLDCDDNNLC
                     TDEVCTKGIGCEYTNNELECDDNNECTVGDTCGGGTCRPGVGELFCDDGNVCTDDACV
                     IGEGCVNVPNNDECEDNNVCTGMGTCVDGSCQRGSYNYDCDDGLQCTADSCHPVDGCQ
                     HSLVNSADCRPQAVIDFPERAATLNGARDITVTGHVDLGLDGIPVPFVTINGNQYALG
                     LDGRFSAPRTSDQGFNGLVVEVEDINGMKDRVVQSYYYSTEWFPFSETDPSVSNVNDG
                     LVLFIGPEVWDDNDTSTYDDVATIISTFVDDIDLMSMISNPVEEGDDGFVCGDHRINI
                     NSITFGTIAVDLTPVNGGLYMKVTIPNIKIGISGKYCHVNFSGTANASSIVVNSTLNI
                     SIDPATGKPVIAMTGTNSSVNGLSININNFPGFLEDILMFFLEDTLTGMIEDTIEDQI
                     AGLIPDLQEALEGLAIDQTFEIPALFDGGSPMTVTLDSGLSSIVFTPAGGTLGMKAAF
                     SAPKGTTHEKLGSLGRANCLLGGLEAMPVFPVGPSSPQLELGLKDDLLNELVFALYWG
                     GALNLPIPAEMLGDSLGDYGITDMVANIDFLLPPVLSACNSQGILKVAVGDVMLDATL
                     KLFGAPLTMKIYVSLMAGANIIARQTETGSTMLGVAVNYPDFIDMEIASLVGNLSGAE
                     DTIKGLVMDTLLPSLLGTLDGVTPLVEFEIPAIDMSSFIDGVPAGSELSIMVREVLRA
                     GAYTVASGTVQ"
BASE COUNT         1910 a         2865 c         2600 g         1770 t
ORIGIN      
        1 ccggcaacgg ccgggccgac gcggcgaacg ctttcatcgg aaccaacaac catgaacagc
       61 ccttcgctcg cgccgccggt aagcagagca agcgcttccg gacgcgcctc atggaaccgc
      121 tcggcaacct gtcgaagaaa atccccgtcg gcctcgtcct gatgatagac ggcaacctga
      181 ccggaacctg catcctcaag catcctctcg accaccaggc cggcgagcct tgcggaaagc
      241 tggcgaaggg aggtgtcgag atcgcgcccg gacttcttca gggcttctat cgatgcgatg
      301 aaatccgatg gccccgtggt cagggccgcg ccaatcgctg attcccgctc gattgtctgc
      361 tcgaaaatcc gccttgccct tgaaccggcc gcatagaaga cgcgaactcc acgagcaagt
      421 ttttcagttc caaggatctt gacgacctgg atctggccgg tggattgaac atgcgtcccg
      481 ccgcaggtat taaggtcgat gccttcgatg ccgaccaacc tgaaaggtcc ctcaaggccg
      541 tccggaagca acctgctgcg aacgttcatg gacgacatct gttcccttga aacgaatgaa
      601 attgaaactg gcagatcatc gaaaaccgcc tggttgacgg cttcttcaag tgtcatcaga
      661 tcctgtctgg acaccgaatc aagttcaaat tcaatgtcgg atcgatccgg attcatgtgg
      721 aatgcgatgg tcttccatcc gaacctggcc gtcgcgatgg ccgtcatcaa gtgctgggca
      781 gtatgctgct gcatgaaatc aaggcgagcc gcccagttca cctcgacatc gacaactccc
      841 gcgaccgggg cctgcagcac atgttcaacg ccaccatcgg gtcgcatgcg cacatcaaga
      901 atcggatacc cggaaaccgt gccgcaatcg gctggctggc caccaccacc cggatagact
      961 accgttcgat ccaggacgac atggaaccgg tccggcgccg tggcgacgca ctcaatcacc
     1021 gtggcccgca ccgacctggc gaaagaatct gtcttgcaca ggatctcggt catgcctttg
     1081 ctccagtgcg gtcaatcggc cgcaaacagc gcgttgacaa aatcctcggg ctgaaacgat
     1141 ctcaggtcgt caacggcctc gcccacgccg acgaaccaga tcggaagatt catgtcacgg
     1201 gcaatggaca caacgacccc gcccctggcg gttccgtcaa gcttcgaaag gcctatgcca
     1261 ctgacattga ccgacttgag gaagacgtcg gcctgcctga cggcgttctg gcccattgtg
     1321 gcgtccagca tcagaagtac ctggtgcggg gcgtccggaa ccgccttgcc aagcacgcgg
     1381 gcgaccttct tcaattcatc cacgaggttg gtatcggtat gaagacgacc ggcagtgtcg
     1441 gcaatcatga tatcgaagcc ttcctcggtc gccttcctgg ccgcatcgaa aaacagtgac
     1501 gacggatcct gccccggccg ccccacgacc acgtcgattc caagcctctc ggcccagatt
     1561 ccaagctggt cggccccggc ggcgcgatag gtatccccgg ccgcaagcaa taccttgcgt
     1621 ccacggttcc tgtaccagtt ggcaagcttc gcgatgttcg tcgtcttgcc ggttccattg
     1681 acgccgacga ccatgacgac gttcagtccg gggcgatcaa gcccgggcat gccgtggggc
     1741 tgggttcgaa tcatgtcgta agtgcgatcc ttcagccatg ccatgacctg gggggccagg
     1801 ggcccgccgg acggctggaa atcgcggcgc aacccttccg acaactccat cgccgtggcg
     1861 acacccatgt cggcggtaag aagggcttcc tcggcggcgg ctatcgtcgc ctcgtcaacg
     1921 gaagagctac gtccgaacgc ctccgaaagc cgggcgatga aaccttccga ccttgtccgg
     1981 gaaagccctt cctgcaaccg gcgagcggac gaaatatcga ccctgaacct tgactgcgcc
     2041 gcaacggaat ccgcggcacc cttcaaggcc ggaacctgct tttccgtgtc cgcggaagcg
     2101 cttccggccc gatcctgatg cctggagccc cgctgggcct gtccaacggc agccgatgcc
     2161 gatgattcgg gtgttcgtga ccgatcgccg cgccccccgc gcttgatggt catctcctga
     2221 tcgaccagtt caaacctgtc atcttcatcg tccgagtcgc gagtcctggc cgaggccgac
     2281 ggtgacagcg aacgtccctt cttcttgcca gccatcttcg ccacgaccgc gacgacaagg
     2341 gcaacgaaac cgattccaag gactatcagg acgatgattg acgggtcaaa agactggctt
     2401 tccatgcgca ctcctggagc attcggcggt cgaaatgcta gtgttgactg aacgggccgt
     2461 caagcccgcg aaggatccct taccacgacc gtcgccacct tgaggtactc gccctctgtc
     2521 caggaaggat tgatcgggtg atcccgaccg gcacccctga cgtcaagcac cgaaatcacc
     2581 cgaccggccg aaacggctgc gtcccgaagc atctcaagga acatgtcgcg acgaacgact
     2641 gacgagcatg aacatgtcac gagaatcccg ccgtccctca aagccttcat cgcggcgaca
     2701 ttaagacggg cgtaatgttt aaggccggac tccatggacg accttcccga tgccagcttt
     2761 ggcgggtcgc agacgaccac gtcgtgttcc cgggcgccgg caaggaactt aagggcatcc
     2821 tgctggacaa aggaaatccg gtcgtcgaag ccgttaatgg cggcgttggc cgcggcagcc
     2881 gcaattgcat ctgcggaaga atcgaccgca gtaatctttt tcgcgccggc agatgcggca
     2941 ttcaaggcaa acccaccgct gaaacagaac aggtcaagga cgtccgcgtc aacacagaga
     3001 tccctgatgg cgagcctgtt ttcccgttgg tcggcataaa atccggtctt ctggccggca
     3061 cccggccttg tcaggaatcc caacccgttc tcaaccacct ttatcggttc cacgggggcc
     3121 gtcccttcgt cttcggccat gatgccttcc agtccgcgga tgccgccgac cacccggaac
     3181 agcacgctga ttccaggccc gagtccagct tcaagacatt cgacaatcag ccgcctgaac
     3241 cgcgacaccc agagggcgga ttccatcacg accacggttc gcgaaaagac atcgatggtc
     3301 atgcccgaca gtccatcgcc ttcactgttg aaaagcctgt aaacatcagt gcgatccgaa
     3361 ggaatgccga atgcagccct gacccggaac gcatcggcga tccttcgcgc gatgatggca
     3421 gcggctgtgt cgggctcccc gggacgggca acaatccgaa ccctgtacga agatccggga
     3481 ttgaaaaacc cgcgaccgat caactggccc atatcatcaa ggacatcgac gaaatccccg
     3541 ggaacgggtt ctccgtgcac gcgcgacacc gatccagaaa aaacgaccgg acagccaaac
     3601 cagaaaggac gcgcccttcc cctggcaaca ctcacggatg ggttcgacat tggatggctc
     3661 ccctgaaatt ggccttaact tggctttccg cccggcgcga tcttacgatt atcaccatga
     3721 aacgggcaat cacactgccg tcgatcattc ttatcgccct tcttggcctt ggtgcgggct
     3781 gccacgccgg aacttccggc acccgggttg ccgcctcatc cgggcatggg aaaaggcacg
     3841 tcccgacggc atcgctgaat tccaaacgac cgaccgtcaa gaattcgggc gcctcgcgga
     3901 cagggtcggc cacaagcgcg gcgaccgaac gctgccggga actccttccc ctgataacca
     3961 gggtcgccag cgaattcgac atcgagacgt cgctgcttgt cggcatcgtt cgagtggaat
     4021 ccaacttcag gaacgacgtg aaatcccctg tcggcgcggt cggcctgacc cagtgcatgc
     4081 cggcaacggc cagggcaaaa cgttgcgggc cgcttaacga tccatacgaa aacctgcgtt
     4141 gcggcgcccg tgtaatcaag gccttcctga aatactacaa ggattccatc tacctggggc
     4201 tttccgggta taacgccggc cacggaatgc cgggcagggc cagacagaat ctggagcttc
     4261 cccagaacgt cgattacgtc gaaaaggtgc ttcaggcatc cgccaggttt gaagaacgcg
     4321 gctgcgactt ctagggtgga aacgatcggt cagtcaacct tgcggccgac gatctcgcct
     4381 ggaatagcag tgaactgaat cccgattgac gccgattcca tgatgccgtc cttgtcgcca
     4441 tcgatatcgg gaataacgac caggtcaagc agcatcataa cggttgcctt gtcaaccggc
     4501 aggtcggccc atgtctcgtc cgggacggcc tgcaccgcgt ccgaaatctg ctgcttcgga
     4561 atcgcgccgc ccaggatgcc ctgcatggac gtgacacgcc cgccggacac ggttacgttg
     4621 gcttccaccc tggtgaaata cagatccacg tgaagattga ttccttcaag cagcggaagg
     4681 tcgaacggga acacgtaatc aagcccgccc gcggtcaact tgttgccgac gatcttcgca
     4741 ttgttcaaag tgaccagcgg cagacactcg tcggtgatca tcatcggctc gacaacatag
     4801 tcgcaatcct gggtcaggaa gttgcaggat tcgtttgtcg gggccagttc accggaatag
     4861 aacgccaggg tataaggcgt cccgtcggtc ttgatctccc ggtgttcaaa cagaagcgtg
     4921 atgctgccct cggccatggc gtccttgatg ccgtcctgag ccgccttcat gccggccacg
     4981 ggaccaagag cgttatcgat gccacagcag caatcctccg cgggcgcgca ggtcgtcggc
     5041 ttcatatcga gatcgaggcc ggaaccggtc tttccgttgg tcgaaatcgc catcgttacg
     5101 aacttggaca ccgccggata gaaggtcggc tgacagacac agccggactc gtcggcgacg
     5161 cagaggccgg cctcgcagtg atcgttgacc gagcaatcgc gaccatcatc acaggcagtc
     5221 ccggaaagga tgtcatgcag gcagagaccg ccgtcacagt aatcggaagt gcactgattt
     5281 ccgtccgaac agttcttcgc cgagccgaag caatcacctt cgtcgcagga atcgccgacg
     5341 gtgcacggat tcccgtcgct gcacgcggcc gtgttgtcgt tgtggaagca ctcgaacgta
     5401 tcggggtcgc aatcgtcatt ggtgcacgga ttattgtcgt cgcaaacgga aacgccgccg
     5461 atgcagcagg gattggttgc caggtcgtgc cagcatccgg tgatgctgtc gcaaccgtcc
     5521 tcggtacaca cgttgccatc gtcgcagttc tggggccggc cgttgcagac gccctcgaaa
     5581 caggaatcct ggaacgtgca ctcgtcgccg tcgtcgcagg cggcttcctc gatcgggctg
     5641 tgctggcatc ccttgacgtc gtcacaggaa tccagggtgc atgagttgcc gtcatcgcag
     5701 gaactgtctt cgggaacgat gcacacgccc tggaagcagt agcctggctt gcacttgtct
     5761 tcaagaacgc attccccgct gctgacaggc gtattgatgc agccgaccgc cggattgcag
     5821 gaatcctcgg tgcacggatc actgtcatcg caccccttcg gcacaatcgt cgaagcaacg
     5881 catacgccgt caacgcaggt gccgccgggc gaatcctcgc aacgtcccag agttaccgtc
     5941 gggtcgcacg cggtttcgtc gggtgccttt gaccattcgc tgacacttgt gcccggcttg
     6001 cagacgaaac agggatcctc gggatccgcg gcgtctttgt cgcggcagat tccgttgatc
     6061 aggcaggttc cggccttcat gacgaacagg cagtttccaa gtccgtcaca tgtgtcatcg
     6121 gtgcaggccc ggccatcgtc gcaggtgtag ggcgtgccgc cgccacattc gcctgactcg
     6181 ccgcatgtct cgccccaggt gcacggattg ccgtcgtcac agggatcgcc ggccaggcag
     6241 tcgccgttgt ccggaagaac cacagaatcg ccgggattcg tatcaatccc gtgatcctgg
     6301 ccgacatcga ccggaccatc atccggctgg ttcaggtcgg gacgagccgt gtccggctgg
     6361 acatcctggc cgttatcgac aggctgattg acatcctcgt agccgacgtc aacgccggtg
     6421 ccctcgtcgg ttcccgattc accgggtcca caggccacga gcatcccgaa tccgatcatt
     6481 gaagcaaaaa gcagcttgcg catctatatc ctccgcaagg gcccggaaag gccccgaaaa
     6541 ctactgaacc gtgcccgacg cgacggtgta cgccccggcg cgcagaactt cccttaccat
     6601 gatgctcaac tcggaaccgg ccggcacgcc gtcgatgaac gaagacatgt cgattgccgg
     6661 gatctcgaat tcgaccagcg gcgtgacgcc gtcaagcgtg ccaagcaggc ttggcagcag
     6721 cgtgtccatg accagcccct tgattgtgtc ctcggccccg gaaaggttcc cgaccagact
     6781 cgcgatctcc atgtcgatga agtccggata gttgaccgca acgccgagca tcgtcgaacc
     6841 ggtctcggtc tgacgggcta tgatgttggc cccggccatc aggctgacat aaatcttcat
     6901 ggtcagcgga gctccgaaca acttcaaggt cgcgtccagc atcacgtcgc cgacagcaac
     6961 cttcaggatg ccctgcgaat tgcaggccga aaggacgggc ggcaacagga aatcgatgtt
     7021 cgcgaccatg tcggtgatgc cgtagtcacc cagggaatcg ccaagcattt ccgccggaat
     7081 cggcagattg agtgcgccac cccagtaaag ggcgaatacc agttcgttca gcagatcgtc
     7141 cttcaatcca agctcaagct ggggcgaact gggaccgacc gggaacaccg gcatcgcctc
     7201 aagaccgcca agcaggcaat tggcacgacc cagcgagccc agcttctcgt gcgtggttcc
     7261 cttcggggca ctgaaggcgg ccttcattcc cagcgtgccg ccggccggcg tgaagacgat
     7321 ggacgaaaga ccggaatcaa gggtaaccgt catcggcgat ccgccgtcga aaagcgctgg
     7381 aatctcaaag gtctggtcga tcgccagccc ctcgagtgct tcctgaagat ccggaatcag
     7441 cccagcaatc tgatcctcga tggtgtcctc gatcatgccg gtcagcgtgt cttcaaggaa
     7501 gaacatcagg atgtcttcaa ggaagcccgg gaagttattg atattgattg acaacccgtt
     7561 gacgctggaa ttggttccag tcatggcgat taccggcttg ccggtggccg gatcgatcga
     7621 gatgttcagg gtcgagttga ccacgatgct gcttgcgttc gcggtgcccg agaagttgac
     7681 gtggcagtac tttccactga tgccgatctt gatgttcggg attgtcacct tcatgtaaag
     7741 cccgccgttt accggcgtca gatcaaccgc aatcgttccg aaggtgatgc tgttgatgtt
     7801 gattcgatga tcgccgcaca cgaagccgtc gtcaccctcc tcgaccgggt tcgaaatcat
     7861 cgacatcaga tcgatgtcat cgacgaaagt cgagataatc gttgcgacgt cgtcgtaagt
     7921 ggaggtgtcg ttgtcgtccc agacctcggg gccaatgaaa agcacaagac cgtcgtttac
     7981 attcgagacc gacgggtcgg tttcactgaa cgggaaccat tcggtcgagt agtagtacga
     8041 ctgaaccacg cgatccttca tgccgttgat gtcttcgacc tcgactacca ggccgttgaa
     8101 tccctggtcg gaagttctgg gagctgaaaa ccttccatcg agtccaaggg cgtactggtt
     8161 accgttaatg gtcacgaacg gaaccgggat tccgtcaagt cccagatcga catggccggt
     8221 taccgtgatg tccctggcgc cgttgagcgt ggcggccctt tccgggaagt cgatgacggc
     8281 ctgtggacgg cagtcggcgc tgttgaccag gctgtgctgg catccgtcaa ccgggtggca
     8341 ggaatccgcg gtgcactgca aaccatcgtc gcagtcgtag ttgtaagatc cccgctgaca
     8401 cgagccgtca acgcatgttc ccatgccggt gcagacgttg ttgtcttcgc actcgtcatt
     8461 gttcgggacg ttgacgcagc cttcgccaat cacgcaggcg tcgtcggtgc agacgttgcc
     8521 gtcatcgcag aagagttcgc cgacgccggg ccggcaggtt ccgccgccgc acgtgtcgcc
     8581 aaccgtgcac tcgttgttgt cgtcacactc gagttcgttg ttcgtgtact cgcagccgat
     8641 gcctttcgtg cagacctcat cggtgcaaag gttgttgtcg tcgcaatcca ggggcaactt
     8701 gccggccagg cattccgaag cctggcagaa gtcgttgacg gtacagggat caccgtcgtc
     8761 gcacgggctc gtattcggta tcgccacgca acccttcgac ggcacgcaac cgtcatcggt
     8821 acagatgttt gaatcgtcgc agaaaagcgg ttccgttccc gacgcgcact cgccaaggaa
     8881 acaggtatcg ccgacggtgc acaggttgcc gtcgtcgcaa ccggcttcgt tgttgatatt
     8941 catgcatccg aactccgggt cgcatgaatc gtcggtgcaa agattaccgt catcgcactc
     9001 gaccgcagtt ccgccgcaga cgttcatcgt gcagatgtcg tcgaaagtgc agcgattgcc
     9061 gtcgtcgcac gcggcaccgt ccgtcgcgtt gttaagacag gttccgttgt cacacacatc
     9121 cgtcgtgcac ggattgccgt cgtcg
//
LOCUS       DAOPPA010000067         9131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8539_length_9131_cov_4.296827, whole genome shotgun sequence.
ACCESSION   DAOPPA010000067 DAOPPA010000000
VERSION     DAOPPA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8539_length_9131_cov_4.296827"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKH54_03375"
     CDS             <1..516
                     /locus_tag="PKH54_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC98958.1"
                     /translation="QGWSFYGKDYPTHAELMAAQERVIARHPGTTFLLIHMGNNAEDL
                     DYVGRLRDSYPNVYTDVSARVPEFGRHPAEKVREFFIRHQDRILFGSDFITAADGSMQ
                     LGSVSEKEPDLVDARVFFERHWRYFETADRQIDHPTPIQGRWKVDAINLPSEVLKKFY
                     VTNARKLIFER"
     gene            667..1605
                     /locus_tag="PKH54_03380"
     CDS             667..1605
                     /locus_tag="PKH54_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC98959.1"
                     /translation="MRNPGTLLLTLILAATVVVSVGCKDKAKSEKTAVSTDVAVLVQK
                     VETADIATVLNYNADLMASSKVRLIALMTDTILSFPWREGDEIAKGQVVAVVKKEALS
                     HGLAQLDAQAVALDVQIANLKSELERARDLLAKGVATQQAVDQLNAQYSATVAQKDAL
                     VAGRRQLAVSAGHAVVKAPVDGVLSGKMYEEGDIASPQMPLGTLLVTDPIKIELKLVE
                     KDISRVSIGQDVDLKLDAWPDRKFTGTVARVFPYVDQATRTNTVEVMVPNPRGEDGAR
                     LLKPGMFGTASLVVERRQGVVTVPESALLIDNKLVA"
     gene            1639..1920
                     /locus_tag="PKH54_03385"
     CDS             1639..1920
                     /locus_tag="PKH54_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98960.1"
                     /translation="MVDSEGLAREKVLVLGAREGDRWEIVDGLAAGESLVVRGQHGLK
                     DGMKVKIVEDGKAPAAEAPATQAPAAEAPAANVDAGSDRPAQAASATTN"
     gene            1952..5119
                     /locus_tag="PKH54_03390"
     CDS             1952..5119
                     /locus_tag="PKH54_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC98961.1"
                     /translation="MKISEISVRRGVTFLMIYLMVVGFGLFSLARLGLDLYPDISFPV
                     VITIVEYQGANPEDIENLITRPLEEAVSAVDGVESVTSESKQGISLVTIEFDWGADME
                     QAETDVRRAIEMIEGYLPEDANAPIIFAFDPSMQPIVMMTLSGPYPLDVLRNVADDEI
                     APLIERLPGVASAEAAGGLVREIHVVLDPKKIDAYNLDVQAVMGAVYAGNNQLPGGYV
                     EQGPLEFDIQVNGKYKSVDEIGDVMVGARQTVAGGMQTIKLREVAQIEDGFQEPRRIL
                     ERDGQSSIWMFVRKQSGANTVQAVKLVMDQIEKVPSQIGRDVEFKVIFNQAEHINASI
                     GNLSSAALQGVVITFFVLLFFLFSMRSSVIVSLAIPISVIATFGLMGQAGMTLNVLSL
                     AGLALAVGMLVDNAIVVMENIFRFRQMGESAWNASIKGASTVGLAVTASTLTTIVVFV
                     PILFVPGIAGVMFNDMAVTICFALSVSLVVALTFIPLAASRLLGGKSAESRIERSTRL
                     QERYLGWLSNAYGRGLDWVLGHRRYVLITIIALLGITGGLWTIMPTGFMAEGDDGFMY
                     VSVEAPVGTNLEETYRTLKEVEARIEQLIPEKDRKMIALDVGVGEGFTAIMSKGVHAG
                     AFRIPLVKPELRQTSLSEYEDILRDELKVFPGVKVTIGSPFNFMGGAGDVELQIRGHN
                     LDDQRRIGLELQEKLRELPELGEIIYSMSDQKPQMEVDFDRQKLSELGLSPGAVSNAV
                     SIAFQGRTVARYSDDGNEYDIKMRYDRKFRRDQNDLKNLPVVTMSGVTVPLENVADIQ
                     TRLGPIDIKRLDQERYTSLNIYLKDKWVGPDGKDVRKDLGKTINKITEVADKIQMPEG
                     FSYKVAGTAEDFQTSFKWMGIALMVSVLLVFMVMASQFESLREPFIIIFTVPLAAIGV
                     VLMFVLTRSTIDVSALIGVIMLVGIVVNNGIIMVDAANQIREEGHGRLRAIALAGRQR
                     LRPVLMTSFTTMLGMVPMALGIGEGAETWAGMGKAVIGGLFTSTFLTLFVVPVMYTVF
                     ARKELKHDKRIFTAGSEQGANRSEV"
     gene            5121..6572
                     /locus_tag="PKH54_03395"
     CDS             5121..6572
                     /locus_tag="PKH54_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC98962.1"
                     /translation="MRKSIILPMIALVLSVAISGKVRASESMPELDGFLPEVVEKVGT
                     LPEPAPGEEIVIADAIEMAAERNTSLEVMKAEVERSRWQTATAWAIIMPMVEASASYV
                     RADHEDVVEFDMSSMLEALGIPVPEGTTSEPTVIRRLDNLNGSLSVSMSLINVAGWYT
                     IAAAQSGDKLARLTYEQVRDQIQSGVVQAFLAAMMTRSVIDLQRSQVVSAAHHLDFAL
                     KKFAAGSGLKIDVVRAEADLVKARQELLNANLAYESARDALGILTGAGGLPVPSGDPQ
                     FDLSSAVPDGRDQIDEAVSRAVEIRNDIKLQQAGLDLAKKSYGASWGGYVPTLVGGWS
                     GSYQFTEPASFGSVDRSRWNLFLNLSIPLFRFDRIGEIKQKKLAVKVAELKVDEAREN
                     AARDIREAERQYRIAVANHEISVKQAELAREAFALVESAYMAGAGSSLEVTDAMRNRL
                     ASEVGALTSELRVRVALVNLNVALGRRVQDVVR"
     gene            6607..7320
                     /locus_tag="PKH54_03400"
     CDS             6607..7320
                     /locus_tag="PKH54_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC98963.1"
                     /translation="MDGSTAVRILLVEDDLRLAALVSEFLSSKGFVVSVEPRGDTAPD
                     RILDENPDLVILDVMLPGLDGLTICRRVRPLYPGPIMMLTALSDEVDEVVGLEIGADE
                     YVAKPVKPRLLLARIQNLLRRVNCCHDEKMVQGDKLLVAGDLRIDPRRRSVEKAGAEL
                     ELTTAEFDLLYYLADRMGQVIERDQIYRDLRGIEWDGLDRSIDLRIARLRRKIGDDAR
                     HPSRIKSVRGTGYLLVDAG"
     gene            7339..8655
                     /locus_tag="PKH54_03405"
     CDS             7339..8655
                     /locus_tag="PKH54_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC98964.1"
                     /translation="MKRYFLRSFFALFAAILLSVVVVSHIAFMSVMRQMEKRPSNTMK
                     LVSMVMAERFAEMTVPQVRDWISTRSVTLDSPIYLTDASDPSIEPGVVSELGHSGMSL
                     RRVWRGTGYSLLVYVPVRVEGGDYILVAGPVERQYGPDMYAFVMMGALILVIMFLSSM
                     VFAFPMWRRLRILEKTARAVADGHLDARIPIPADDGMVGFLASQFNTMVSRIDELLAS
                     QKMMLQAVSHELRTPSARIRFGLEMLESAKDPAERARRLDAIDEDLAELDELVEELLI
                     FNKTEALKHEIERSPVNVSEALGRLIEKRAFLRPGVDVVQDVSDDSIEVMVDQRAFIR
                     AIGNLLSNAMRFSRTKVRIAVRREGDQAMVEVADDGPGVPEDSRSTILQPFKRLDGSR
                     NRESGGAGLGLAIVSSIVNAHRGRIEILEADIGGAAFRTWWPLSGN"
     gene            8799..>9131
                     /locus_tag="PKH54_03410"
     CDS             8799..>9131
                     /locus_tag="PKH54_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98965.1"
                     /translation="MRVFRFMPVMMVLALVAGCGGGPVSEDLAGYDVMADLLPGQDAN
                     SVGELRVETIVSANQAATGEEITVQCRAAGEGSEDVTTGFSVSGDKNTVEGDKVVFTA
                     PGSYVVTCN"
BASE COUNT         1812 a         2464 c         2894 g         1961 t
ORIGIN      
        1 cagggatgga gtttttacgg taaggattat ccgacccacg cggaactgat ggcggcccag
       61 gaaagggtga tcgcgcgcca tcccggaacg acgttcctgt tgattcacat gggtaacaac
      121 gcggaagatc ttgattacgt cggcaggctt cgtgattcct atccgaacgt atatacggac
      181 gtttcagcca gggtgccgga gttcggcagg catccggccg agaaggtgag ggagttcttc
      241 atcaggcacc aggacaggat acttttcgga tccgacttca tcacggcggc cgatggttcg
      301 atgcagctcg ggtcggtatc cgaaaaggaa ccggatctgg tcgatgcaag ggtattcttc
      361 gaacggcact ggcgctattt cgagacggcc gatcgacaga tagaccatcc gacaccaatc
      421 cagggcaggt ggaaagtcga tgcgatcaac ctgccgtccg aagtcctgaa gaagttctat
      481 gtgaccaatg cacgcaagct gattttcgag cgctgatgcc atcgggcgca tacaaaccgt
      541 tacaaatccc cacatcgatg taagatgacg tcctggcgcg caatgacaat acttgtcacg
      601 attgcggcac gcgcgtggga cgctgccgcg cagtccaatc cggattgaat gcacagagga
      661 caaatcatga gaaatccagg aacacttctt cttactttaa tccttgccgc caccgtcgtt
      721 gtttccgtcg gctgcaagga taaggcgaag tccgagaaga ccgctgtatc gaccgatgtc
      781 gcggtcctgg ttcagaaggt cgaaacggcc gatatcgcga cggttctgaa ttacaacgcc
      841 gacctgatgg catcttcgaa ggttcggttg attgcgctga tgaccgatac gatcctttcg
      901 ttcccgtggc gcgagggcga cgagatcgcg aagggtcagg tggtcgcggt ggtcaagaag
      961 gaggcgcttt cccacgggct tgcccagctt gatgcccagg cggttgcgct tgatgtccag
     1021 attgccaact tgaagagcga gcttgagcgg gcccgggatc ttcttgcaaa gggtgtcgcc
     1081 acccagcagg cggtagatca gcttaatgcg cagtattcgg ccacggtcgc ccagaaggac
     1141 gcgctggtgg ccggaaggcg ccagcttgcg gtcagcgccg gccatgccgt ggtgaaggcg
     1201 ccggttgacg gcgtactttc tggcaagatg tacgaagagg gcgatatcgc ttcaccccag
     1261 atgccgctgg gaaccctgct ggtgaccgac ccgatcaaga tcgagttgaa actggtggaa
     1321 aaggacattt ccagggtgtc gattggtcag gatgtggatc tgaagcttga cgcatggccc
     1381 gacaggaagt tcaccggaac cgtcgcccgg gttttcccgt acgtcgatca ggccacaagg
     1441 acgaacaccg tcgaggtgat ggttccgaac ccacgcggcg aagacggagc ccgtcttctc
     1501 aagcccggca tgttcggcac ggcgagtctt gtggtcgagc gcaggcaggg tgttgtaacg
     1561 gttcctgaaa gcgccctgct tatcgacaac aagctggtcg cttgacagca ggccggccag
     1621 cttcttcgca aggcgttcgt ggtcgattcg gaagggcttg cgcgtgagaa ggtgctggtg
     1681 ctgggcgcaa gggaaggcga ccgctgggag atagtcgatg ggctcgctgc cggtgaaagt
     1741 cttgttgtca ggggacagca cggtctgaag gacgggatga aggtcaagat cgttgaagat
     1801 ggaaaggcgc cggcggcgga agcaccggcc acgcaggcgc cggctgcgga agcgccggcg
     1861 gcaaacgttg atgccgggtc ggatcggccg gctcaggccg cgtccgcgac gaccaactaa
     1921 cctgccttcc gggacacgtg gagatgaaca gatgaagatt tccgaaattt ccgtcagaag
     1981 gggcgtaacc tttctgatga tctaccttat ggttgtcggg ttcggtcttt tctcgctggc
     2041 caggcttgga ctggaccttt atcccgatat cagctttccc gtcgtcataa cgattgtcga
     2101 gtatcaggga gcaaatcccg aggacatcga gaaccttatc acgcgcccgc ttgaggaggc
     2161 ggtgtccgcg gttgacggcg tcgagtccgt cacgtcggag tcgaagcagg gtatcagcct
     2221 tgtgaccatc gagttcgact ggggcgctga catggagcag gccgagaccg acgtcaggcg
     2281 cgccatcgag atgatcgagg gatatcttcc cgaggatgcg aacgccccga tcatcttcgc
     2341 gttcgatcct tccatgcagc cgatagtcat gatgactctt tccggcccat atccgctcga
     2401 cgtgctgcga aacgtcgccg acgacgagat tgccccgctg atagagcgtc ttcccggcgt
     2461 cgcgtccgca gaggcggccg gcggcctggt gcgcgaaatc catgtcgtgc tggatccgaa
     2521 gaagatcgac gcttacaacc tggatgtcca ggccgtaatg ggcgcggttt acgcgggcaa
     2581 caaccagctc cccggcggct atgtcgagca gggtccgctg gagttcgaca tccaggtcaa
     2641 cggcaagtac aagtcggtgg atgagatcgg cgacgtcatg gtgggtgcgc ggcagacggt
     2701 tgccggcggc atgcagacga tcaagctgcg cgaggtcgcg cagattgagg acgggttcca
     2761 ggagccgcga aggattctgg agcgcgacgg ccagtcttcg atatggatgt tcgtgcggaa
     2821 gcagtccggc gccaacacgg tccaggcggt caagctggtc atggaccaga ttgagaaggt
     2881 gccgtcccag atcggtcgtg acgtcgagtt caaggtgatc ttcaaccagg cggagcacat
     2941 caacgcatcg atcggaaacc tgtccagcgc ggcgcttcag ggcgtcgtca tcactttctt
     3001 cgtcctgctg ttcttcctgt tctcgatgcg aagttccgtc atcgtttcgc tggccatccc
     3061 gatttccgtc atcgcgactt tcggattgat gggccaggcc ggaatgaccc tgaacgtgct
     3121 ttcgcttgcc ggtcttgcgc ttgcggtcgg catgcttgtc gataacgcca tcgtcgtcat
     3181 ggagaacatt ttcaggttca ggcagatggg cgagtcggcc tggaatgcaa gcatcaaggg
     3241 tgcatcgacg gtcggcctgg ccgtcaccgc ctcgacgctg acgaccatcg tcgtattcgt
     3301 cccgatcctt ttcgttccag gtatcgcggg cgtcatgttc aacgacatgg ccgtgaccat
     3361 ctgcttcgcc ctttccgttt ccctggtggt tgcccttacc ttcataccgc ttgccgcttc
     3421 aaggcttctt ggcggaaagt cggccgaatc acgaatcgag agatcgacgc gccttcagga
     3481 aaggtatctg ggctggcttt ccaatgccta tggccgcgga ctggactggg ttctgggtca
     3541 ccgaaggtat gtgcttataa ccattatcgc tcttcttgga atcaccggtg gcctgtggac
     3601 aatcatgccg accggtttca tggccgaagg cgatgacggc ttcatgtatg tttcggtcga
     3661 ggccccggtc ggaaccaatc ttgaagagac ttacagaacc ctcaaggaag tcgaggcccg
     3721 aatcgaacaa ctgatcccgg aaaaggatcg gaagatgatc gctcttgacg tcggcgtcgg
     3781 cgaagggttc accgcaatca tgtcgaaggg cgttcatgcg ggtgccttcc ggattcccct
     3841 ggtcaagccg gaactgcggc agacctccct gtccgagtac gaggacatcc ttcgcgacga
     3901 actgaaggtg ttccccgggg tgaaggtaac cattggcagc ccgttcaact tcatgggcgg
     3961 cgccggcgat gtcgagttgc agattcgcgg gcacaatctg gatgaccagc ggcgcatcgg
     4021 cctggaactg caggagaagc ttcgcgagct tccggaactt ggcgaaatca tatattccat
     4081 gtcggatcag aagccgcaga tggaggtcga tttcgacagg cagaaactgt ccgaactcgg
     4141 gctttccccc ggtgcggtca gcaacgcagt ttcaatcgcg ttccagggca ggacggtcgc
     4201 aagatactcc gacgacggaa acgagtacga catcaagatg cgttacgacc gcaagttccg
     4261 gcgggatcag aacgacctga agaatcttcc cgttgtgacg atgtcgggcg tgaccgttcc
     4321 tctggaaaac gtagccgaca tccagacccg acttggaccg atcgatatca agcggcttga
     4381 ccaggaacga tacaccagcc tgaatatcta tctgaaggat aagtgggtgg gcccggacgg
     4441 caaggatgtc aggaaggatc tcggcaagac aatcaataag attaccgagg ttgccgacaa
     4501 gatccagatg ccggaaggtt tttcgtacaa ggttgccgga acggccgaag acttccagac
     4561 atcgttcaag tggatgggta ttgcgctgat ggtgtcggtg ctgcttgtct tcatggtcat
     4621 ggcaagccag ttcgagtcgc tgcgcgagcc gttcatcatc atcttcaccg tcccgctggc
     4681 ggcaatcggc gtggtgctga tgttcgtcct cacccgttca accatcgacg tttcggcctt
     4741 gattggcgtc atcatgctgg tcggcatcgt cgtcaacaac ggtatcatca tggttgacgc
     4801 ggcaaaccag attcgcgagg agggccacgg acggctgcgg gccatcgcgc ttgccggccg
     4861 ccagaggctt cgtcccgtcc tgatgacgtc gtttacaacc atgctcggca tggtgccgat
     4921 ggcactgggc atcggcgagg gcgccgaaac ctgggccggg atgggcaagg cggtcatcgg
     4981 tgggcttttc acatcgacgt tcctgactct gttcgtggtt cccgtcatgt acaccgtgtt
     5041 tgcccgaaag gaactgaagc atgacaagag aatcttcacc gccggttctg aacagggcgc
     5101 gaaccggagc gaggtctgaa atgaggaaga gcatcatatt gccaatgatt gcccttgtcc
     5161 tttcggttgc catttctgga aaggttcgcg cgtcggaatc catgccggag cttgacggat
     5221 tccttcccga ggtggtcgaa aaggtcggga cgctgcccga gcccgcgccg ggcgaggaaa
     5281 tcgtcatcgc ggacgccatc gaaatggctg ccgaacgcaa caccagtctg gaagtgatga
     5341 aggccgaggt ggagagatcg aggtggcaga cggctaccgc gtgggccatc atcatgccga
     5401 tggtggaggc gtcggccagc tatgtccggg cggatcatga ggacgtggtc gaatttgaca
     5461 tgagttccat gctggaggcg cttggaatcc ccgtcccgga aggaaccact tccgagccga
     5521 cggtaatcag gcgcctcgac aaccttaacg gttcgttatc cgtttcgatg tcgctgatta
     5581 acgtcgcggg gtggtacacg attgccgctg cgcagtccgg ggacaagctt gcaaggctga
     5641 catacgagca ggtcagggat cagattcaat ccggggttgt ccaggcattc ctggccgcga
     5701 tgatgaccag gtcggtaata gatcttcaaa gatcccaggt tgtttcggcc gcgcaccacc
     5761 tggatttcgc gctgaagaag tttgccgccg gttccggcct gaagattgac gtggtgcgtg
     5821 ccgaggccga ccttgtcaag gcaaggcagg agcttctgaa cgcgaacctg gcctatgaat
     5881 cggcgcgcga cgccctcggg atactgaccg gagccggcgg gcttcccgta ccttcgggtg
     5941 atccgcagtt tgatctttcg tcggccgtcc cggatggccg ggatcagatc gacgaggcgg
     6001 tcagtcgggc cgtcgagatt cgaaatgaca tcaagttaca gcaggcaggt cttgatcttg
     6061 ctaagaagag ttatggggca agctggggcg gatacgtccc gaccctggtt ggcgggtggt
     6121 ccggctctta tcagttcacg gaaccggctt cgttcggatc ggttgaccgt tcgcgctgga
     6181 atcttttcct gaatctttcc attccgcttt tcaggttcga caggattggg gaaatcaagc
     6241 agaagaagct ggcagtcaag gttgccgagt tgaaggttga cgaggccagg gagaacgcgg
     6301 cccgcgacat ccgcgaggcc gagcggcagt acaggatcgc cgtggccaat cacgagattt
     6361 ccgtgaagca ggcggaactt gctcgagagg catttgccct tgtggaatcg gcctacatgg
     6421 ccggggccgg ttcgtccctc gaggtgaccg acgccatgcg caatcggctt gcatccgaag
     6481 tcggggcgct gacttccgag ctgcgggtca gggttgcgct tgtcaacctg aacgtggccc
     6541 tgggcagacg cgttcaggac gtggtcaggt gatttgccgg caattgacct cccgtggagg
     6601 gtgatcatgg atggctcgac ggccgtaagg attctgctgg tcgaggatga cctgaggctt
     6661 gccgccctgg tttccgagtt tctttcgtcc aagggattcg tcgtttccgt cgagccgagg
     6721 ggtgacacgg cgccggacag gattctggac gaaaatccgg atctcgtaat cctggacgtg
     6781 atgcttccag gtcttgacgg gctgacaatc tgtcggcgcg taaggccgct ttacccaggc
     6841 ccgataatga tgttgacagc gctttcggac gaggttgacg aggttgtcgg gctggagata
     6901 ggggccgacg agtatgtggc caaaccggtc aagccccgcc tgcttcttgc caggattcag
     6961 aacctgctgc ggcgggtcaa ctgctgtcac gatgaaaaga tggttcaggg cgacaagttg
     7021 ctggtggccg gtgatttgag gattgatccc cgccgtcgtt cggtggaaaa ggccggcgcg
     7081 gaactggagc ttacgacagc cgagttcgac cttctttact atcttgccga caggatgggg
     7141 caggtcatcg aacgcgacca gatttatcgc gatttgcgcg gcatcgagtg ggacgggctg
     7201 gatcgttcga ttgacctgag gattgcgcgc ctccgtcgca agattggcga cgatgcgcgg
     7261 cacccgtcgc ggatcaagtc cgtacggggc accgggtacc tgcttgtgga tgccggataa
     7321 ccgtcgggcg gaagacatat gaaaaggtat ttccttcgtt cattcttcgc gcttttcgcg
     7381 gcaattcttc tgtcggtcgt agtcgtcagc catatcgcct tcatgtccgt catgcgtcag
     7441 atggaaaaaa ggccaagcaa caccatgaag cttgtgtcca tggtgatggc cgagcgcttt
     7501 gccgagatga cggttcccca ggtaagggat tggatctcga ccaggtcggt gaccctggac
     7561 tcgcccatct atcttactga tgcctcggat ccgtcgattg agcccggcgt ggtttccgag
     7621 ctcggtcatt ccggcatgtc gcttcggcgg gtttggcgtg gtaccggata cagcctgctg
     7681 gtttacgtgc cggtaagggt ggagggtggg gactacattc tcgtggccgg tcccgtcgag
     7741 cggcagtacg gtcccgacat gtatgccttc gtgatgatgg gtgcgctgat ccttgtgatc
     7801 atgttccttt cttcgatggt ttttgcattt ccgatgtggc gccgtcttcg aattcttgag
     7861 aagacggcca gggcggttgc cgacggacac ctggacgcga ggattccgat tcccgccgac
     7921 gacggcatgg tcggattcct ggcctcccag ttcaacacga tggtgtccag gattgatgaa
     7981 ctgcttgcct cgcagaagat gatgctgcag gcggtttccc atgaactgcg caccccgtcc
     8041 gcaaggatcc gattcgggct ggagatgctt gaatccgcga aggatccggc agagcgcgcg
     8101 cgcaggctgg acgccatcga cgaggacctg gccgaacttg atgaacttgt cgaggaattg
     8161 ttgatcttca acaagaccga ggcgttgaag cacgaaattg aaaggagtcc ggttaacgtg
     8221 tcggaagcct tgggcaggtt gatcgaaaag cgcgcgttcc tgcggcccgg cgtcgatgtt
     8281 gttcaagatg tcagcgacga ctcgattgag gtaatggtcg atcagcgcgc cttcatcagg
     8341 gccatcggta atcttctttc caacgcgatg cgtttttcca ggacgaaggt tcggatcgcg
     8401 gtgcgtcgtg aaggcgacca ggcaatggtc gaggtggctg acgacggacc gggcgtgccg
     8461 gaagattcca ggtcgacgat tcttcagcca ttcaagcgtc ttgacggcag caggaaccgc
     8521 gaatccggcg gagccggcct tggccttgcg atagtttctt ccatcgtcaa tgcccacagg
     8581 gggcggattg agatcctcga ggccgacatc ggcggtgccg ccttccgcac ctggtggcct
     8641 ctttcaggca attgatcccc ggccattcga accgattttg cgttcatggt gcgtcttgcc
     8701 ggggcacggc tggttataac acttcatttc atgacggacg ttaagtaaaa taccgcaggc
     8761 cgtccaggct tgtcgtcttc taacgatgag gtttccccat gcgtgttttt cgtttcatgc
     8821 cagtgatgat ggtgcttgcc cttgttgccg ggtgcggcgg cggtccggtt tccgaagatt
     8881 tggccggtta tgacgtgatg gccgacctgc ttccgggcca ggacgcgaat tcggtcggcg
     8941 agcttcgcgt cgagacaatc gtgtcggcca accaggcggc cacgggcgag gaaatcacgg
     9001 ttcagtgccg cgccgccggg gaaggctccg aagacgtgac gaccggtttt tccgtttctg
     9061 gcgacaagaa caccgttgaa ggcgacaagg tcgttttcac cgcacccggt tcctacgtcg
     9121 tgacctgtaa c
//
LOCUS       DAOPPA010000068         9069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8614_length_9069_cov_3.892944, whole genome shotgun sequence.
ACCESSION   DAOPPA010000068 DAOPPA010000000
VERSION     DAOPPA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8614_length_9069_cov_3.892944"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="PKH54_03415"
     CDS             <1..1819
                     /locus_tag="PKH54_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOC98966.1"
                     /translation="PDLVTRNVSQARDAFEGSGPRFTWLLRNKGGFEFEDVTESSGFT
                     ATRDVEGTQGRVTHVVVWGDVDNDGDLDAFSGRSMTSKPTGASDNMDDRAELLLNNGD
                     GTFSLAAEQPFRWVTRDPKLAENLQMYDPNLAASFLDYNRDGSLDLFLGYAMYGQAFV
                     QDMLFAGDGTGAFSDVTEEAGLKLKVPLAYPYTADPAYKNGTAVRNTWGTTVTDINND
                     GWPDIMCSVYGRYFNALWLGGPEGFSDWSHESGYASDHREDWTTNLNARCYCKLVREV
                     PQYSGYPAEGCEGVQDPPETFRCVNPDELRWNHNTDRNPYRLGGNTFSTVSGDIDNDG
                     DMDLFTFEIVHWDVGDTSDPAELLVNDGSPKPIFSRPGAGALGMARDWDGHGDFNAGD
                     MTGALFDFDNDGRLDVLIASSDYPYTRAFLFHQKDDGTFEEVPAGVGIDQPHAHGVAV
                     ADFDLDGDLDVVLGHSTARCDSSPDECYSENAGFGKITYVQQFHAFRNEIGQSGNWTR
                     INLIGGAGSNRAAIGAVVKVTAGGVTRMREVGGGHGHVGIQHEMTQHFGLGSSCNIDR
                     IEVRWPDAAGTVEVFRNVRGNYVVDIEQGAGAVRYHLPE"
     gene            1932..4880
                     /locus_tag="PKH54_03420"
     CDS             1932..4880
                     /locus_tag="PKH54_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98967.1"
                     /translation="MFHKGRLESLLLVASVAVLAACGPSGGPNPQEDAVQDIHETDFG
                     YDHGTLPDSGGDTVIDARDSGIDDADVVDDVPDVGPDFDVADAEDDGIDSDAADVPAD
                     PGDAGDDGDIGSADDGVEPACEDDGLECTVESRGDDGVCVSTLMESWCLIDDVCYGPE
                     AVHPDNRCLECAPLIDPLAWTVIVGRDCDDGLKCTTGDVCGDDGICAGVAVACPDDGN
                     SCTVEACSPDTGSCFTTTLQNGAACQDDLNECTIDRCQGGTCVHAVRNGGTCSDGDMC
                     TYGDACDAQGVCRGNEKVCVDDGNVCTENGCDPLTGNCVAVKIDDNRKCEDYNWCTLN
                     DGCRKGVCTGDLRDCSDPDPCTFDTCVDGPAGYCSNEPVFGSCEDGNLCTRGETCNIA
                     GICEGGQPIVCDDDNKCTDDSCDPAIGCVFTPNYSFCDDGNPCTSGDLCDEFGACVGE
                     PIPCSDDNDCTSDYCFLGTCRHDNKVNGIDCDDGDVCTTGGKCNFGSCVPSGDLDCND
                     NNPCTVDLCDPVEGCYTEVLSCDDGNPCTDDSCDSVTGCRHDANTAICNDGDACTEAD
                     RCSGKQCKGTPLDCSDGDTCTLDKCADGICSNPPNTGACDDGNACTTGERCFDGVCMN
                     GSPVDCYDGSDCTADSCNPASGCVNTPISGSCDDGTVCTLNDQCSSGICVGTPINCND
                     GNFCTNDLCHPLDGCYHENNALLCDDGNPCSTPDQCGGGTCNGQLVFRNPVIKGASFV
                     IGADGYAGSGLDVDENAGTCLPSNKCDGGIDNAFASMALMVKDQFNPDLVERTASGDL
                     AMFLEPESPGPVGSPFEMKMFFGHRTAPETCNPLTSGCNYLAFKADTIGSCTVKYAFG
                     NASTASSHISAGGKTTETVVYFMIGSYAMPIALKWARIEADVTISGNSVVSGSGVIGG
                     VINKVDFKYALQAVPDAYFSPYTKSQVILNVDQTLPMDADADSNGVKESSSIGFKFTI
                     VQGNVVGRDN"
     gene            complement(4886..5563)
                     /locus_tag="PKH54_03425"
     CDS             complement(4886..5563)
                     /locus_tag="PKH54_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98968.1"
                     /translation="MKRLIICLFVAAIGTGCRSAPPEPAVVTPQMEAELLAGFNAIIS
                     AHDNCADMDAFMRMHTTQAQAAMTALDSVADPAFLEPDFNNDGPNRGALWVCLVARHA
                     GMTSGNVKVIRHVIDPRQKLARVIFELRGGEYGFPMARQDGTWRSPFPGQVFLAAQFN
                     GWLNEIKSRLPEDRHELLLPKLSQATKVLGGYQPNWALYPELSEKETDELQLKIKENV
                     RKAVGQE"
     gene            complement(5598..6974)
                     /locus_tag="PKH54_03430"
     CDS             complement(5598..6974)
                     /locus_tag="PKH54_03430"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC98969.1"
                     /translation="MNEKVLDYVKNNQDRFLAELFDWLRIPSVSALPEHNGDVRRAGE
                     WVVSKLEAIGFENVGLKETTGHPAVYGDWLHAPGKPTILVYGHYDVQPVDPIEKWHSN
                     PFEPVVKDGFIYGRGTSDDKGQVATHLFALESILKVTGRLPCNVKVFIEGEEEGGTGG
                     TADFVTANRDLLACDAVAISDTGWYSPDLPTIVYALRGLAYFHVDVFGPDRDLHSGSY
                     GGMLQNPLNAVAKIIAALQDADGRITIPGYYDDVVELTELERAEFRKVESPEDQVARE
                     IGVPVLWGEKGFSAIERNWARPSFDVNGIWGGFSGEGAKTVIAAEGGFKVSSRLVAGQ
                     DPEKVAVQFKDHIERVCPPGVRVKVTYLHGATPVMVPVDSPFITAGQNAIEKTFGKRP
                     VLVREGASIPITATFLTELKAPSIMVGYGLLEDNIHSPDEKFKLSHFQKGIECGVVLY
                     EEFASVSR"
     gene            complement(7137..8009)
                     /locus_tag="PKH54_03435"
     CDS             complement(7137..8009)
                     /locus_tag="PKH54_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOC98970.1"
                     /translation="MLTMIAAIGVLTAAAGGADARPAPKQGQLRRFTYQRCTSETLEA
                     VSRLLGVSQTDLEKWNRGSIVSTGGRKSFRKGVSRVIYYSRAAFPRESVGRPSSGTLV
                     GGMNLDCDGDDRGQGWVVSVDRSNLYGTPETIRNIYNAAKRYRAYFRDRGLKFVPLSI
                     GDISRPSGGPLPPHVSHQSGRDVDVGIILKSGNASGRFLHANPSNMDPLRTWVMLKSF
                     IDTSDVRYIFIHRGLADALKTYISKIYARQRPLLRKYLGAFAPDGVIRPDAEHSSHVH
                     IRFRCPKGDRRCVE"
     gene            8247..>9069
                     /locus_tag="PKH54_03440"
     CDS             8247..>9069
                     /locus_tag="PKH54_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC98971.1"
                     /translation="MKKWLIACAVVFAAGCSSSGDVALYGDCVSSDDCSAGQVCGPFV
                     CSSSQLGCWAMWGCTTEEECGRESVCHKDPEGCLTGACGKVVKVAAGSFTMGCDKGEN
                     ETDEVCHPMSRPAHAVTFAKPFYIDKHEVTVAEYAACAAEAVYDKDGYVTFACDLPKL
                     SVGDCNWKGREDHPVTCVSWNQATSYCKWAGRRLCTEAEWEYAARGPSGKVYPWDGVE
                     ECESNDVCKQTNSIHVTLTTEPSGCGDTLTTPAVPPVEEASEAEGMPAPEPFCDVT"
BASE COUNT         1781 a         2656 c         2786 g         1846 t
ORIGIN      
        1 gccggacctt gtgaccagaa acgtgtccca ggcacgcgac gcctttgaag ggagcggccc
       61 cagattcaca tggctgctgc ggaacaaggg cgggttcgag ttcgaggacg tgaccgaatc
      121 cagcggattc accgcgacca gggacgtcga ggggacgcag ggccgcgtca cgcacgttgt
      181 cgtatggggc gatgtcgata acgacggcga tctggacgcg ttttccggaa gatccatgac
      241 ctcgaagccg acaggggcct ccgacaacat ggacgatcgc gccgaacttc tgctgaacaa
      301 cggcgacggg acgttttcgc ttgcggccga gcagcccttc cgctgggtga caagggatcc
      361 gaagctggcc gaaaacctcc agatgtacga cccgaacctt gcggcttctt tccttgatta
      421 caatcgcgac ggaagtctgg atttgtttct cgggtacgca atgtacgggc aggcctttgt
      481 ccaggacatg ctgtttgccg gcgacggtac gggcgcattt tccgacgtta ccgaggaggc
      541 cgggctcaag ctgaaggttc cgctggcgta tccctacacc gccgaccccg cttacaagaa
      601 cggaaccgcc gtcaggaaca cgtggggaac aacggtcacg gacatcaaca acgacggctg
      661 gccggacatc atgtgctcgg tctatggacg ttacttcaac gcgctctggc ttggcggccc
      721 cgaaggtttt tccgactggt cgcatgagtc cgggtacgca tcggatcaca gggaggactg
      781 gacgacgaac cttaatgcca ggtgttattg caagctggtc agggaggttc cacagtactc
      841 cgggtatccg gccgaaggtt gcgagggcgt tcaggatccg ccggaaacct tccgttgcgt
      901 caacccggac gagcttcgct ggaaccacaa cacggatcgg aatccctacc ggctgggtgg
      961 caacaccttt tcaacggtgt ccggggatat cgacaacgac ggtgacatgg atctgttcac
     1021 gttcgagatt gtccactggg atgtcggcga cacttcggat cccgccgaac tgctggtcaa
     1081 cgacggctcc ccgaaaccga ttttctcaag gccgggcgcc ggggccctgg gcatggcacg
     1141 ggactgggac ggtcacgggg atttcaacgc tggcgacatg accggcgccc tgtttgattt
     1201 cgacaacgat ggaaggctgg acgtcctgat cgccagctcg gattatccgt atacgcgtgc
     1261 attccttttt caccagaagg acgacggaac cttcgaggag gttccggccg gcgttggaat
     1321 cgaccagccg catgcccacg gggtcgcggt ggccgatttc gatctcgatg gcgatctgga
     1381 cgtggttctg gggcacagca cggcacgctg cgacagcagt ccggatgagt gctattcgga
     1441 gaatgccggt tttggaaaga tcacgtatgt acagcagttt cacgcattca ggaacgaaat
     1501 tggccagtcg ggaaactgga ccaggatcaa cctgatcggt ggcgccggtt cgaaccgagc
     1561 cgcaatcggc gcggttgtga aggtgaccgc cggcggggtt acccggatgc gcgaggttgg
     1621 cggcggccac ggccatgtcg gaatacagca cgaaatgacc cagcacttcg gtctcggctc
     1681 ctcgtgcaac atcgacagga tcgaagtcag gtggccggat gcggccggga cggtcgaagt
     1741 cttcaggaac gtgcgcggta attacgtggt cgatatcgag cagggtgccg gcgcggtccg
     1801 gtaccacctg cccgagtaat gtgcgaaggg ctgtccgcgc gccgcatccg cccgattctc
     1861 caagtcacta aaattctgaa atcggcactc aaagagtgta aaataccgcg gactgtactg
     1921 cggggcttca catgtttcac aaaggtcgcc tggaatcact gcttctcgtc gcgtctgtcg
     1981 cggtactcgc agcgtgcggg ccttccggcg gcccgaatcc ccaggaggac gcagtccagg
     2041 acatccatga aacggacttt ggttacgacc atggaacctt gcccgattcc ggcggcgata
     2101 ccgtaatcga tgcccgcgat tcaggcatcg atgatgccga tgtcgtggat gacgttccgg
     2161 atgtcgggcc ggatttcgac gtggccgatg ccgaagacga cggaatcgat tcggatgccg
     2221 ccgatgttcc cgcggatccg ggtgatgccg gtgatgacgg cgatatcggt tctgccgacg
     2281 acggcgtcga gcctgcctgc gaggacgacg ggctggaatg cactgtcgag tcgcgtgggg
     2341 atgatggcgt ctgtgtttcg accctgatgg aatcctggtg ccttatcgac gacgtctgtt
     2401 acgggcctga agccgtccat ccggacaacc gctgtctgga gtgcgccccg ttgattgatc
     2461 cgctggcctg gaccgtgatc gtcggccgtg actgcgatga tggtctcaaa tgcacgaccg
     2521 gcgatgtctg cggtgacgac ggcatctgcg ccggtgtcgc cgtggcctgt ccggatgacg
     2581 gaaattcatg caccgtcgag gcttgcagcc cggacacggg cagttgcttc accacgaccc
     2641 tgcagaacgg cgccgcatgt caggatgatt tgaatgaatg cacgattgat cgatgccagg
     2701 gcggaacatg cgtgcacgcg gtaagaaacg gcggtacatg cagcgacggg gatatgtgca
     2761 cctacggaga tgcctgcgat gcgcagggcg tctgccgagg gaacgaaaag gtctgtgtcg
     2821 atgacggaaa cgtctgcacg gaaaatggat gcgatccgct taccggcaac tgcgttgcgg
     2881 tgaagattga cgacaaccgg aaatgcgagg actacaactg gtgcacgctc aatgatggct
     2941 gcaggaaggg cgtctgcacc ggcgacctgc gggattgcag cgacccggat ccctgcacgt
     3001 ttgatacgtg tgttgacggt cctgccggct actgctcgaa cgagccggtt ttcggctcat
     3061 gcgaggacgg caacctgtgc accaggggcg agacttgcaa tatcgccggg atctgcgagg
     3121 gcggacaacc gatcgtctgc gacgacgaca acaagtgtac cgacgattcc tgcgacccgg
     3181 ccatcggctg cgtgttcacc cccaactatt ccttctgcga cgacggcaat ccgtgtacgt
     3241 ccggcgatct ctgcgatgag ttcggcgctt gcgtcggcga gcccattccc tgttcggacg
     3301 acaacgattg caccagcgac tactgcttcc tcgggacatg ccgccacgac aacaaggtca
     3361 acggaatcga ctgcgacgac ggcgacgtct gcacgacggg cggaaaatgc aatttcgggt
     3421 cgtgcgtgcc ttccggtgat cttgactgta acgataacaa tccgtgcacc gtcgatctct
     3481 gtgatccggt cgaaggctgc tacacggaag tcctcagttg cgacgacggc aatccgtgca
     3541 ccgatgattc gtgcgattca gtcacggggt gccgccacga tgccaacacc gccatatgca
     3601 atgacggcga cgcatgcacg gaagcagaca ggtgctccgg caagcagtgc aaaggcacac
     3661 cactggactg ctcggacggc gatacatgca cgcttgacaa gtgcgccgac ggcatctgtt
     3721 cgaatccacc gaataccggc gcatgcgacg acgggaacgc ctgtaccacc ggcgagcggt
     3781 gctttgacgg tgtctgcatg aacggttccc cggtcgattg ctatgacggc agcgactgca
     3841 ccgctgattc ctgcaatccc gccagcgggt gcgtgaacac gccgatttcc ggttcgtgtg
     3901 acgacggaac cgtgtgcacg ctgaacgatc agtgttcgtc aggcatctgc gtcggtaccc
     3961 cgatcaactg caacgacggg aatttctgca cgaacgacct gtgccatccg ctggacggct
     4021 gctatcacga aaacaacgcc ctcctgtgcg acgacggcaa tccctgttcg acgccggatc
     4081 agtgcggcgg cggaacatgc aacggccagc tcgtcttccg aaatcctgtg atcaagggcg
     4141 cgtctttcgt gatcggggcg gacggatacg cgggcagcgg tctggacgtg gatgagaatg
     4201 ccggcacgtg tctgccatca aacaagtgcg acggcggaat cgacaacgcg ttcgcctcga
     4261 tggccctgat ggtgaaggac cagttcaatc cggatctggt tgaacggacg gcctcgggcg
     4321 atctggcgat gttccttgag cccgaaagcc ccgggccggt cggttcgccc tttgaaatga
     4381 agatgttctt tggccaccgc accgcgcccg agacgtgcaa tccgctgacg tcgggctgca
     4441 actacctggc gttcaaggcc gacaccatcg gttcatgcac cgtcaagtac gcgttcggaa
     4501 acgcgagcac ggcgtcctca cacatttccg cgggcggcaa gacgactgaa acggtcgtgt
     4561 acttcatgat cggttcgtat gcgatgccga ttgccctgaa atgggcgcgc atcgaggcgg
     4621 acgtcacgat cagcggcaac tccgtcgttt ccggatccgg cgtcatcggc ggcgtcatca
     4681 acaaggtcga tttcaagtac gcgcttcagg cggttccgga cgcctatttc agtccctata
     4741 cgaagtcgca ggtaatcctg aatgtcgatc agacgcttcc gatggacgcc gacgcggatt
     4801 caaacggcgt caaggagtct tcgtcaatcg ggttcaagtt cacgattgtc cagggcaacg
     4861 tggtcggccg cgacaactga tttgcttact cctgcccaac cgccttccgc acgttttcct
     4921 tgatcttcaa ttgaagttca tcggtttcct tttcggaaag ttccgggtag agcgcccagt
     4981 tcggctggta accgccaagc acctttgttg cctgcgacag cttcggaagc agcagttcat
     5041 gcctgtcctc gggaaggcgc gacttgattt cgttcagcca gccattgaac tgtgccgcca
     5101 ggaatacctg gcccggaaag ggggaacgcc aggttccgtc ctgtcgcgcc atggggaacc
     5161 cgtactcgcc gccgcgaagt tcgaagatca cacgcgcaag cttctggcga ggatctatca
     5221 cgtgacggat gaccttgacg ttgccgctgg tcattccggc atgcctggcg acaaggcata
     5281 cccacagtgc gccgcggttg ggcccgtcgt tgttgaagtc cggttccagg aaggccggat
     5341 cggcaacgga gtcaagcgcg gtcatcgcgg cctgggcctg cgtcgtgtgc atacgcatga
     5401 acgcatccat gtcggcgcaa ttgtcgtggg ccgaaattat ggcgttgaat ccggccagga
     5461 gttcggcttc catctgcggg gtgacgaccg ccggctcggg cggcgccgac ctgcatcccg
     5521 tgccgattgc agcgacaaag agacagatga tgaggcgttt cattccatga ccccctgtaa
     5581 ggagtgatgc gggagcgtca ccttgaaact gacgcgaatt cctcgtaaag cacgacgccg
     5641 cattcgatgc ctttctggaa gtgggacagc ttgaatttct cgtccgggct gtggatgttg
     5701 tcttcaagca gtccgtagcc aaccatgatt gacggagcct tcaattcggt caggaatgtg
     5761 gcggtgatcg ggatcgaagc gccttcccgg acaagaaccg ggcgctttcc gaaagtcttc
     5821 tcgatagcgt tctggccggc cgtgatgaac gggctgtcaa ccggcaccat caccggcgtg
     5881 gcgccgtgca ggtaagtgac cttgaccctg acgccaggcg ggcagacccg ttcgatatgg
     5941 tccttgaact gaacagcgac cttttcggga tcctggccgg ccaccaggcg gctgctgacc
     6001 ttgaatccgc cctcggcagc aatgacggtt ttcgcgcctt cgccggagaa tccgccccag
     6061 atgccgttca catcgaagct tggcctggcc cagttgcgtt cgattgccga gaaccccttt
     6121 tcaccccaca gaaccgggac gccgatttcg cgcgcgacct ggtcttccgg tgattcgacc
     6181 ttgcggaatt cggccctttc cagttccgtc agttcgacaa cgtcgtcgta gtacccggga
     6241 atcgttatcc tgccgtcggc gtcctgaagc gcggcgatga ttttggccac cgcgttcagc
     6301 ggattctgaa gcattccgcc atagctcccg ctgtgcagat cccgatcagg accgaagaca
     6361 tcgacgtgga aatatgcaag tccccgtagc gcatagacaa tcgttggaag atccggcgaa
     6421 taccatcccg tatccgatat cgcgacggcg tcgcacgcca gcagatcccg gttcgcggtg
     6481 acgaaatccg ccgtcccgcc cgttccgcct tcctcttcac cctcgatgaa caccttcacg
     6541 ttgcacggca gccgtccggt taccttcagg atggattcca gggcgaaaag gtgcgtcgcg
     6601 acctggccct tgtcgtccga tgttccgcgt ccatagatga agccgtcctt tacgaccggt
     6661 tcaaatggat tcgaatgcca tttctcgatt ggatccaccg gttgtacgtc gtaatggccg
     6721 taaaccagga ttgtcggctt gcctggagcg tgcagccagt cgccatagac ggccgggtga
     6781 ccggtcgttt ccttcaggcc aacgttttcg aaaccgatgg cttcaagctt cgacaccacc
     6841 cattcgcccg cgcggcgaac atccccgttg tgttcgggaa gggcggaaac gctgggaatc
     6901 ctgagccagt cgaaaagttc ggcaaggaac ctgtcctgat tgttttttac gtaatccagt
     6961 actttttcgt tcacggcagc tacccccgga accgcggcga cgtgccgccg cgatggtgaa
     7021 agaatggatt tgcgggacaa aacccttcac gaaggaagtt acccgttgcc tgtctggtgg
     7081 tcaatacctg tgtggcgcga acgggcggga cgactctgga catctcggca ccgcggctat
     7141 tcgacgcacc gcctgtcacc tttcgggcat cggaacctta tgtgcacgtg agatgaatgt
     7201 tccgcatccg gcctgatgac tccgtcgggc gcgaatgcac cgagatactt cctgagaagg
     7261 gggcgctgcc gcgcgtatat ttttgatatg taggtcttca aggcgtcggc caggccccgg
     7321 tggatgaata tgtagcgcac gtcggacgta tcgatgaacg acttcagcat cacccatgtc
     7381 cgcagtggat ccatgttcga cgggtttgcg tgcaggaagc gtcctgaagc gtttcccgac
     7441 ttcaggataa tgccgacatc gacgtccctt ccgctctggt gcgaaacgtg gggggggagc
     7501 gggccacccg acggtcttga aatgtcgccg attgaaagcg ggacgaactt caggccgcga
     7561 tcccggaagt aagcccggta tcgcttcgcg gcgttgtaaa tgttccttat ggtctcaggt
     7621 gttccataga ggttgctccg gtcaaccgag acgacccagc cctggccgcg gtcatctccg
     7681 tcacagtcga ggttcattcc gccgacaagg gttcccgacg acggccgccc cacagattcg
     7741 cggggaaatg ccgcccttga atagtagatg acccttgaaa cgcctttgcg gaatgacttg
     7801 cgcccccctg tcgagacgat tgagcccctg ttccactttt ccagatccgt ctgcgacacg
     7861 ccaagaagac gggatacggc ctcgagcgtc tcgctggtgc accgctgata tgtgaaacgc
     7921 cggagctggc cctgcttcgg ggccgggcgg gcatccgcgc cgccagccgc cgccgtcagc
     7981 accccgatgg cggcaatcat tgtcagcatt cggatcagcc tgtcaggccg aggtactttc
     8041 atggctctcc gatgtcgggc cctggacggg cgacttcttt gacccgccgg ccgggtccga
     8101 tccacattgt aacggccagg ttccgccgat gcggaatttt tcgcttgcgt ctccacattt
     8161 cggagccttc cgcacatgac gttcaacatt gacattcatt tccggcgtga tacctttctg
     8221 cgtggttaac tgtggagtca ttgccgatga agaagtggct gattgcatgt gcggtcgttt
     8281 ttgccgccgg ttgttcaagt tccggtgatg tcgcgctcta tggcgattgc gtttcctccg
     8341 atgactgttc ggctggacag gtttgcggac ctttcgtctg ctcatccagc cagttgggct
     8401 gctgggcgat gtggggatgc accacggaag aagaatgcgg ccgcgaatcg gtctgccaca
     8461 aggatcctga aggatgtctg accggtgcat gcggaaaggt cgtcaaggtt gccgccggct
     8521 ctttcacgat gggctgcgac aagggtgaaa acgagaccga cgaggtctgt catccgatgt
     8581 cccgtccggc acatgcggtc actttcgcga agccgttcta catcgacaag catgaagtca
     8641 ccgtggccga atatgccgca tgcgcggcgg aagccgtcta tgacaaggac ggttacgtga
     8701 cgtttgcctg cgatcttcca aaactttcgg ttggcgactg caactggaag ggacgcgagg
     8761 atcatccggt cacctgcgta tcctggaacc aggccacgag ttactgcaag tgggctggaa
     8821 ggcggctctg caccgaggcc gagtgggagt acgcggccag gggaccgtcc ggaaaggtct
     8881 atccgtggga tggcgttgaa gagtgcgagt ccaatgatgt ctgcaagcag acgaattcga
     8941 ttcacgtgac ccttaccacc gagccgtccg gttgcggcga cacactgacg acacccgcgg
     9001 ttcccccggt tgaggaagcg tccgaggcgg agggcatgcc cgcccccgag ccgttctgcg
     9061 atgtcaccg
//
LOCUS       DAOPPA010000069         8876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8854_length_8876_cov_4.149076, whole genome shotgun sequence.
ACCESSION   DAOPPA010000069 DAOPPA010000000
VERSION     DAOPPA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8854_length_8876_cov_4.149076"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..5652
                     /locus_tag="PKH54_03445"
     CDS             277..5652
                     /locus_tag="PKH54_03445"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOC98972.1"
                     /translation="MKARWLLAGMLMAVLLSFGCATEVGTIDRTQADKLEKKLFAGVW
                     FMTQEVIDIGYNGAFSFVGEMNFGATSKVVFDIQKDMLVVYPVTEYIQDSEKEFHRQK
                     IYRYWDDACMTSDSEQYGCVDGIDNLNDPEKVCCFVDMYVGQPIAAFDITSHFEVQRQ
                     YNSGTGYQSPILEENSYDKQWWQRKWFRVNWTRNNINDFTFMARMINQSPVDYYVQEF
                     EDGNPDAPTFNEDYIDIVTKVFGEPASTGSCDIYGVSYGDCASAVVKFRTAFRKADVA
                     QNYEPQRYDNEDEQKLYGFFLTSRNSWDEDYGITEAGKVTYANRWNLWQNSFEDVEVE
                     IELQAEDGTTYKAPKPCFKDLEDTGCDTVLKDGVKEFCRADDWFQHGNCVVRKTVPYT
                     QRGLKPIVYSVSTSLPEDFWPATRKMAEEWSDAFKDTVAWLYLWEEKGIIHGSERVRA
                     CTTNADCATHAVVDLMFDLDQVDQPSMLPTNAGTGKPIYSSVAVTAVITPMGKAFTMP
                     DWGFPAGTALTGNCAVRFVNVSDGDATLELNGTALFQDVAPMTESSFNPAAPAHQTVT
                     AGNITLAVGDKEVSLACASNRLVTVVFNGTDLAVSDVSKYMAKGLRVINASSMSLDIS
                     VNGSIRAYAVEPGQNSGYTSIGGGISLKDNPEDQLLAAGFWPQRLVATEAGSRGDVSC
                     LKYEGRSVCAGYNSPINADDFARVAEIKAKLPEIFVLCHNSYMPADGEGNLNDDEYKS
                     NLYAPWFEVNNASDEDLAKLPEKNPCVDFLFNAEKMTEDQKMEQALAVKKAGDSRYSM
                     VYWVSETQFSSPLGYGPSAADPDTGEIFWGVANIYGAFIYYYQNLYRDLFDLLRGRLN
                     TEDYVTGENIRQMVLNKDKWEEDTEGGTINRKATMVSLADGARDIRAQVDATDLSRQL
                     SADLNWKAPRGPSFQEMISTIRTLQRDKNVMATLPTQPVSFGSNRLSLLKGTRFEEML
                     NSGEVAYAAQAAGKGSPLEWMNLETINKAEIERHRILGKNNYCFYEFEDEGLIGIVKA
                     WACMPGDTRPICDASNFDDLARDNMDANHATCCIDDSETLGKLVGYRMYEGTVIHEVG
                     HTIGLRHNFSASTDVFNYFDGYYDIREREAVPCVVSDECDQVSGNYCKDGFCRQNLVD
                     TCSIDSDCGVIDGTGAVTYLDNFKCIDNACHDVTRCGLHNECPTGSFCEGDTKLCKDR
                     SSGALLAAPVAGGEQLVKQFIPRAAMTEREADLSRTLYQYTSIMDYGQRFNSDLLGIG
                     KYDRAAIRFGYGRLTDVYTNLSRLHKAAHNQTQLYSDPDDTTNAYNLDSMYWNSGIYF
                     SQFYYLNNQIGIENNRNDGQYYMNRFPAPYDLVRNEHTLTFNYYRQEHDWSYVTVPYN
                     YLADEWRGNVGCYTWDTGVDILEIIHSMNIQLSQYYLMDAFKRERYGFGLGGNPMGYM
                     ARVQERYMDPMRGAAMYYALYAHLLKNYSWRAIWANARLMGWALRRASEYGFEILANT
                     MASPAPGSYKLNIDTNMYENISFDVNAQGSELNIPLGDGKYPYTTFWDGAGYYYFDHA
                     QFYGSFWEKMAALMTLTDSTVYFTSNYVGEQLDIGVGTSLGFNTMYPALLVELLGGLT
                     AEEPKYFAFRGGDGKATPHEYFDVRNMDAYTVQPSPYFTPLPTSDAKVVEPSISNLTM
                     KVNAMLYGMTYLPASFDPSFVDSFLVCLDGTPECNEIGPESGIVAQTFEDPASGKVYK
                     AWGPTYRNDWFAPNLSVIARANTLAAELATASEDDKPAIQEQIRKLVEVLDQMRGIYQ
                     TFSAIKI"
     gene            5721..>8876
                     /locus_tag="PKH54_03450"
     CDS             5721..>8876
                     /locus_tag="PKH54_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98973.1"
                     /translation="MKNFARTAMITALAAIMFAGCAQDVGTIDRVKDNYVKKFDLLYN
                     PDGTRKEWFFRLTVTETPYASAYSFNGAQHPIERGVFEIQEDYLYFYRTYTFVENEYD
                     DNPRVDTHTKLTNLDGTDYLLNGKPVWRDKNSPVIAYPVASHFDIIWDYNPVTGEPGN
                     VLSENSSDRMWYERDFARIDWGSNMLPTYVQKFQSDFDYQTILVDSSSIEEMDINPST
                     GYMSFVNSVIAGVQTDYIDGFGEIPICWYYPWYAGGLYECVSEEITMRGSFMAVDTTR
                     EQEYHPIQYTDYDQARFGFFRAERLRWDHDYGLTYHLFQRYATRWDIWKKDADGNIIG
                     VRPVVYHVSPGYPNNMVSEARAIAVEWSKAFDATVKGVTGKNVNELPAWTQHGPQMLD
                     ADGNEVFVDHMFIICENNQADLDLRDRSEGTLETNEAICGSLAEEKVNGDLRYSFLYS
                     VNEPGQGTPYGYGPPAWDPISGRIVSGNAYVYNAAFREGAVRALDQIELLAGVKSFRE
                     IANADYIEEAMRNERLEQFSYWRNGFTNEDAQKMVDRLVTPAVADALSSSVRKSDQSP
                     VAGRMNIISKVPNLESLFVNKDVRWLFKDPSLFGRDGNALNAKFGEEDNSTISVSSKL
                     HLRNWANGTGTLEEIKEFQKMAASGIDRAEFYDGALMGLAKMYKSKYDAAVCDALKGQ
                     SGLVYDFGRFNDSKPCNVADLIEQLRAALVVASQGTPFGYKTISNPTPLELAAYDPTV
                     AASQKAMKDVLAEIRPQYIDELQQLIFYGVGIHGVGHTVGLRHNFEASSDAMNFPKAY
                     WDLKVKKVGDKYEGVGLFGETPEQVAKEMRAMQYSSVMDYYAKFNMPSLGLGMYDIAA
                     VKYAYGNLVEVYRNKPDTTDYDAYINADPTAGLPENTPSVKDRGEDFGIALRRVHHTE
                     WPNMFGDTAAMYDRVDVDKFEVYGAKCESEGASCGNGKVCKKFYEGLLCSAQDKVIAP
                     YRFGSDEIAFYLPTVSPWDEGVDAYEIVRNLNEIYDNYWVFRGYWHQDPTFWPSYYDG
                     WVRRIFAMMKIQFQYWAVNFKV"
BASE COUNT         2034 a         2645 c         2491 g         1706 t
ORIGIN      
        1 agcaggcggc tcatatccgc cgtgtcgggg gttcaaatcc ctccttcgcc accagtaatc
       61 cagttatcac cgtattttca gaaccttttt cgcaactttt tgcatacgct tcagcagtcg
      121 aatccagcaa ccaacgagca agtcaaaaaa aattccccac acctcaccag gatcaagttt
      181 tttcctttta tttgtccatg aaaggttgta aaagttatcg gtttcatact ggggtgcatg
      241 cacgcactcc attgatctca ggttggaggt tacataatga aggcccgctg gctccttgca
      301 ggaatgctca tggccgtgct tttgtccttc ggttgcgcca ccgaagtcgg gacgatcgac
      361 aggacacagg ccgacaaact cgaaaagaag ctcttcgccg gtgtctggtt catgacccag
      421 gaggtcatcg acatcggata caacggtgcc ttcagcttcg taggcgaaat gaacttcggc
      481 gccacgtcga aggtcgtttt cgacatccag aaggacatgc tggtagtgta tccggtcacc
      541 gaatacatcc aggattccga gaaggaattc caccggcaga agatctaccg atactgggat
      601 gacgcctgca tgaccagtga ttccgagcag tacgggtgcg tcgatgggat cgacaatctg
      661 aacgacccgg aaaaggtgtg ctgctttgtc gatatgtacg tcggccagcc gatcgcggcc
      721 ttcgacatca ccagccactt cgaagtccag cgtcagtaca attccggaac cgggtaccag
      781 agcccgatcc tggaagaaaa ctcgtacgac aagcagtggt ggcagcgcaa gtggttccgg
      841 gtcaactgga cgcgcaacaa catcaacgac ttcacgttca tggcccgaat gatcaaccag
      901 agcccggttg attactacgt ccaggaattc gaggacggca acccggacgc gccgactttc
      961 aacgaagatt acatcgacat cgtcacgaag gtgttcggcg agcccgccag caccggaagc
     1021 tgcgatattt acggtgtttc atacggcgat tgcgcctcgg ccgtcgtcaa gttccggacc
     1081 gccttccgga aggccgacgt cgcccagaat tacgaaccgc agcgttacga caacgaagac
     1141 gagcagaagc tttacggttt cttcctgact tcgcgcaact cgtgggatga agactacggc
     1201 atcaccgagg ccggcaaggt tacatacgcc aaccgatgga acctgtggca gaactcgttt
     1261 gaggatgtcg aagtcgaaat cgaactccag gcggaagacg gaaccaccta caaggccccg
     1321 aagccctgct tcaaggatct tgaggacacc ggctgcgata ccgtcctgaa ggatggcgtg
     1381 aaggaattct gccgcgccga cgactggttc cagcatggaa actgtgtcgt ccggaagacg
     1441 gttccctaca cccagcgcgg tctgaagccg attgtgtact cggtctcgac cagcctgccg
     1501 gaagacttct ggccggccac caggaagatg gctgaagaat ggtcagacgc attcaaggac
     1561 accgtcgcgt ggctgtacct gtgggaagaa aagggtatca tccacggctc ggaacgcgtc
     1621 cgcgcctgca ccaccaatgc cgactgcgcg acccatgcgg tcgttgacct gatgttcgac
     1681 ctggatcagg tcgatcagcc ctcgatgctc ccgaccaacg cgggcaccgg caagccgatt
     1741 tactcgtcgg tcgcggtaac cgcggtcatc accccgatgg gaaaggcatt cacgatgccg
     1801 gactggggct tcccggccgg caccgccctg accggcaact gcgcggttcg gttcgtcaac
     1861 gtatcggatg gcgatgcaac gcttgagctc aacggaaccg ccctgttcca ggacgtcgcc
     1921 ccgatgaccg aaagcagctt caacccggcg gcccctgcac atcagacggt tacagccggg
     1981 aacatcacgc ttgccgtggg cgacaaggaa gtatctctgg cctgcgcgag caaccggctg
     2041 gtcacggtcg ttttcaacgg caccgacctg gcggtctcgg acgtcagcaa gtacatggca
     2101 aagggtctgc gcgtaatcaa cgcttcttcc atgtccctcg acatctcggt caacggctcg
     2161 atccgcgcct atgcggtcga acccggccag aactcgggat acacgtcaat cggcggcggc
     2221 atctccctga aggacaaccc ggaagaccag ctcctggcgg ccggtttctg gccccagcgc
     2281 ctggtggcga ccgaggccgg ctcgcgcggc gacgtctcct gcctgaagta tgaaggccgc
     2341 agcgtctgcg ccggttacaa ctcaccgatt aacgccgacg acttcgcccg cgtcgccgaa
     2401 atcaaggcga agctgcccga aattttcgtc ctgtgccaca acagctacat gcccgccgat
     2461 ggcgagggca acctgaacga cgacgaatac aaatcgaacc tgtacgcgcc ctggttcgag
     2521 gttaacaacg catcggacga agatcttgcg aagcttccgg aaaagaaccc ctgcgttgat
     2581 ttcctcttca acgcggaaaa gatgacggaa gaccagaaga tggagcaggc actcgcggtc
     2641 aagaaggccg gcgacagccg ctacagcatg gtctattggg tttccgagac gcagttctcc
     2701 tccccgcttg gatacggccc ctcggcggcg gatccggaca ccggagaaat cttctggggc
     2761 gtcgccaaca tttacggcgc cttcatttac tactaccaga acctttaccg cgacctgttc
     2821 gatcttctgc gcggcaggtt gaacaccgag gactacgtca ccggtgaaaa catccgccag
     2881 atggttctta acaaggataa gtgggaagaa gacaccgagg gtggcaccat caaccgcaag
     2941 gccacgatgg tcagtctggc cgacggcgcc cgcgatatcc gcgcccaggt cgatgcgacc
     3001 gatctgtccc gccagctttc ggcggatctt aactggaagg ccccgcgcgg accgtcgttc
     3061 caggagatga tttcgacgat tcggaccctg cagagggaca agaacgtcat ggcgacgctg
     3121 ccgacccagc cggtcagctt cggttccaac cgcctgtccc tgctgaaggg cacccgtttc
     3181 gaggagatgc tgaattccgg tgaagtcgct tacgcggcgc aggcggccgg aaagggcagc
     3241 ccgcttgaat ggatgaacct ggaaacaatc aacaaggcgg aaatcgagcg ccaccgcatc
     3301 ctcggcaaga acaactactg cttctatgaa ttcgaggacg aaggcctgat tggtatcgtt
     3361 aaggcctggg cctgcatgcc tggcgacaca cgcccgatct gcgacgcgtc gaacttcgac
     3421 gacctcgctc gcgacaacat ggacgccaat cacgcgacgt gctgcatcga cgattccgag
     3481 accctcggca agctggtcgg ttaccgcatg tacgaaggca ctgtcatcca cgaagttggt
     3541 cataccatcg gcctgcgtca caacttcagc gcatcgaccg acgtgttcaa ctacttcgac
     3601 ggatactacg acatccgcga gagggaggct gttccgtgcg tggtcagcga cgaatgcgac
     3661 caggtttcgg gcaactactg caaggacggc ttctgccgcc agaaccttgt tgacacgtgc
     3721 tctattgact ccgactgcgg cgtcatcgac ggaaccgggg ccgtgaccta tctcgacaac
     3781 ttcaagtgca tcgacaacgc gtgccatgac gtgacgcgct gcggcctgca caacgaatgc
     3841 ccgacaggct ccttctgcga aggcgacacg aagctttgca aggacaggtc gtccggcgcc
     3901 ctgctggcgg cccccgtcgc cggtggcgag cagcttgtca agcagttcat cccccgcgcg
     3961 gcgatgaccg agcgcgaggc tgatctgagc cggacgctct atcagtacac gtccatcatg
     4021 gattacggcc agaggttcaa cagcgacctc ctgggcatcg gcaagtacga ccgcgctgcc
     4081 atcaggttcg gttacggacg ccttaccgac gtctatacca acctgtcgag gctgcacaag
     4141 gccgcgcaca accagaccca gctctactcc gatcccgacg acacgaccaa cgcgtacaac
     4201 ctggactcga tgtactggaa ctcgggtatc tacttcagcc agttctatta cctgaacaac
     4261 cagatcggca tcgagaacaa cagaaacgac ggccagtact acatgaaccg gttcccggcg
     4321 ccgtacgacc tggtccgcaa cgaacacacg ctgaccttca actactaccg ccaggagcac
     4381 gactggtcgt acgtgaccgt accttacaac tacctcgccg acgaatggcg tggaaacgtc
     4441 ggctgctaca cctgggatac cggcgtcgat atcctcgaga tcatccacag catgaacatc
     4501 cagttgtcgc agtactacct gatggacgcg ttcaaacgcg aacgttacgg cttcgggctt
     4561 ggcggcaacc cgatgggcta catggccagg gttcaggagc gctacatgga tccgatgagg
     4621 ggcgccgcga tgtactacgc cctttacgcc cacctgctga agaactactc gtggcgcgcg
     4681 atctgggcta acgccaggct gatgggctgg gcgctgcgcc gcgcctccga gtatgggttc
     4741 gaaatccttg ccaacacgat ggcttcgccg gcccccggtt catacaagct gaacatcgac
     4801 accaacatgt acgagaacat cagcttcgac gtgaacgccc agggcagcga actgaacata
     4861 ccgctcggcg acgggaagta cccctacacc accttctggg atggggcagg ttactactac
     4921 ttcgaccacg cccagttcta cggttcgttc tgggaaaaga tggccgcgct gatgacgctg
     4981 accgactcga ccgtgtattt cacgtcgaac tacgttggcg agcagcttga tatcggcgtc
     5041 ggtaccagcc ttgggttcaa cacgatgtac ccggccctgc tggtcgagct gctgggcggc
     5101 ctgactgccg aggaaccgaa gtacttcgcg ttccgcggcg gcgacggcaa ggcaacgccg
     5161 cacgaatact tcgacgtccg taatatggac gcctacacgg tccagccctc gccttacttc
     5221 accccgcttc cgacatcgga cgcgaaggtg gtcgaaccgt caatctccaa cctgacgatg
     5281 aaggttaacg cgatgcttta tggaatgaca tacctgccgg catcattcga tccttcgttc
     5341 gtcgattcat tcctggtatg ccttgacggc accccggaat gcaacgaaat cggcccggaa
     5401 tccggaatcg tcgcccagac gttcgaggat cctgccagcg gcaaggtcta taaggcctgg
     5461 ggcccgacct acaggaatga ctggttcgcc ccgaacttgt cggtgattgc cagggcgaat
     5521 actctggcgg cggaactcgc cacggcgtcc gaggacgata agccggcaat ccaggaacaa
     5581 atcaggaagc tggtcgaggt tcttgaccag atgcgcggca tttaccagac gttcagcgca
     5641 atcaaaatct gatgggggat gacggaatga aaaatactgc ttcaatgaaa gtcatcaacc
     5701 tcgtcggagg aggaaccgtg atgaagaact tcgcaaggac ggcgatgatt acggcgctgg
     5761 ccgccattat gtttgccggt tgtgcccagg acgtgggaac gattgaccgc gtcaaggaca
     5821 actacgtcaa gaaattcgac ctgctttaca accccgacgg cacgaggaag gaatggttct
     5881 tccgcctgac ggtcacggaa acgccgtacg cttcggcgta ttccttcaac ggcgcccagc
     5941 acccgatcga acgcggcgtt ttcgaaatcc aggaagacta cctgtacttc tacaggacct
     6001 ataccttcgt ggaaaacgaa tacgacgaca atcctcgcgt cgatacccac accaagctga
     6061 ccaacctgga cggcacggat tacctgctta acggcaagcc ggtgtggcgg gacaagaatt
     6121 cccccgtgat cgcttacccg gtggccagcc acttcgacat catctgggat tacaacccgg
     6181 tgaccggcga acctggcaac gtcctttcgg aaaactcctc ggacaggatg tggtacgaac
     6241 gcgatttcgc acgtatcgac tggggctcga acatgctgcc gacatacgtg cagaagttcc
     6301 agagcgactt cgactaccag acgatactgg tcgattcgtc cagcatcgag gaaatggaca
     6361 tcaatccatc gaccggatac atgagcttcg tgaactcggt tatcgcaggc gtccagaccg
     6421 actacatcga cggtttcggc gaaatcccga tctgctggta ctacccctgg tatgccggcg
     6481 gactttacga atgcgtgtcg gaagaaatca cgatgcgcgg atccttcatg gctgttgata
     6541 ccacccgcga gcaggaatac cacccgatcc aatataccga ctacgatcag gcaagattcg
     6601 gattcttccg cgccgaaaga ctgcgctggg atcacgatta cggcctgacg taccatctgt
     6661 tccagagata cgcgacgcgc tgggacatct ggaagaagga tgccgatggc aacatcatcg
     6721 gcgtgcgccc ggtcgtgtat cacgtcagcc ccggttatcc gaacaacatg gtctcggaag
     6781 ccagggcaat cgcggttgaa tggtccaagg cctttgacgc gaccgtgaag ggcgttaccg
     6841 gtaagaacgt caacgaactt ccggcctgga cgcagcacgg cccgcagatg ctggacgcgg
     6901 acgggaacga agtgttcgtc gatcacatgt tcatcatctg tgaaaacaac caggccgatc
     6961 tggatcttcg cgatcgcagc gaaggcacgc ttgagacgaa cgaggcaatc tgcggttcgc
     7021 tcgccgagga aaaggtcaat ggcgatcttc ggtacagttt cctgtactcg gtcaacgaac
     7081 ctggccaggg cacgccgtac ggttacggtc cgccggcctg ggatccgatc agcggacgta
     7141 tcgtttccgg caacgcctac gtttataacg cggccttccg cgagggtgcc gtccgcgcac
     7201 tggatcagat cgaactcctg gcgggcgtca agagcttccg cgaaatcgca aacgccgact
     7261 acatcgagga agcaatgcgc aacgaacgcc tcgagcagtt cagctactgg cgcaacggct
     7321 tcacgaatga agacgcccag aagatggttg acagactggt gaccccggcc gtggccgacg
     7381 cgctctcctc gtcggttcgc aagtccgacc agtcaccggt ggccggacgc atgaacatca
     7441 tctccaaggt tcccaatctt gaaagcctgt tcgtcaataa ggacgtccgc tggctgttca
     7501 aggatccttc gctctttgga agggatggca acgcactgaa cgcgaagttc ggcgaagagg
     7561 ataattcgac catctccgtg tccagcaagc tccatctgcg aaactgggcg aacggcacag
     7621 gaacccttga agagatcaaa gaattccaga agatggcagc atccggcatc gaccgggccg
     7681 aattctatga cggcgccctg atgggattgg ccaagatgta caagtcgaag tacgacgccg
     7741 ccgtctgcga cgcgctgaag gggcagtcgg gccttgttta cgattttggc aggtttaacg
     7801 attccaagcc ctgtaacgtt gccgacctga tcgagcaact ccgtgcggca ctggttgtcg
     7861 ccagccaggg tacaccgttc ggatacaaga ccatttccaa tccgaccccg cttgaactgg
     7921 ccgcctatga cccgaccgtc gcggcttcgc agaaagccat gaaggacgtc cttgccgaga
     7981 tcaggccgca gtacatcgac gaactgcagc agttgatttt ttacggcgtc ggtattcatg
     8041 gagtgggcca tacggtcggt cttcgccaca acttcgaagc ttcgtccgat gccatgaact
     8101 tcccgaaggc ctactgggat ctgaaggtca aaaaggttgg cgacaagtac gagggcgtcg
     8161 gcctgtttgg agagaccccg gaacaggtgg cgaaggagat gcgtgcgatg cagtactcct
     8221 ccgtcatgga ctattacgcc aagttcaaca tgccgtcgct gggcctcggt atgtacgaca
     8281 ttgcggcggt caagtacgcg tacggaaatc tcgtcgaagt gtatcggaac aagccggata
     8341 cgacggatta cgacgcatac atcaacgccg acccaaccgc cggtcttccc gaaaacaccc
     8401 catccgtcaa ggatcgcggc gaggatttcg ggatagcgct tcggcgggtt caccacaccg
     8461 aatggccaaa catgtttggc gataccgccg ccatgtacga ccgtgtcgat gttgacaagt
     8521 tcgaggttta tggcgccaag tgcgaaagcg agggcgcatc ctgtggcaac ggcaaggtct
     8581 gcaagaagtt ctatgaaggc cttctctgct cggcacagga caaggttatc gctccgtacc
     8641 gcttcggttc ggatgaaatc gctttctatc tgcccacggt cagcccatgg gacgagggtg
     8701 tcgatgcata cgaaatcgtt cgcaacctga acgaaatata tgacaactac tgggttttcc
     8761 gcggttactg gcaccaggat ccgacgttct ggccgtcgta ctacgatggt tgggttcgcc
     8821 gcatcttcgc gatgatgaag atccagttcc agtactgggc agtcaatttc aaggtc
//
LOCUS       DAOPPA010000070         8814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_8920_length_8814_cov_3.868821, whole genome shotgun sequence.
ACCESSION   DAOPPA010000070 DAOPPA010000000
VERSION     DAOPPA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_8814_cov_3.868821"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PKH54_03455"
     CDS             <1..1323
                     /locus_tag="PKH54_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98974.1"
                     /translation="LALPDKCHFINIQEGIDIAASRGLSQVLVSQGVYNESIVLHDGV
                     GVFGRYDSSRNWERAPSKVSQIRGGTLAVTADGIASPTILQGLVITSESASAPGSSSI
                     AVAARNGSGLSIQACTVQAGAGAPGAAGAAGNAGQAGSNGSSGSAGCEYVCVFQIGDE
                     NCFDMCGQCERPLGGAGGAGAAGANGGKGGDGGPYATAGGAGGSGLGNGAGTGGSGGP
                     MTQNGSRGHDGLDGVSGSNGIGGGASGVFSAGGYAAADGSPGTDGTDGGGGGGGGAGG
                     GDNPQIWELNLCGTYGSSGGGGGGGGGGGKGGIGGGGGGASVGILASAGSVAIEQTMI
                     LTGSGGAGGAGGFGGSAGSGGTAGLAGDHGDGDGQGVGAAGGVGGKGGAGGAGGGGGG
                     GPSFGIVCVDASYVSSGMTVLTGSGGPGGSSTVNTGASGSSGQSFGCP"
     gene            complement(1349..2164)
                     /locus_tag="PKH54_03460"
     CDS             complement(1349..2164)
                     /locus_tag="PKH54_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOC98975.1"
                     /translation="MGDIDNSSANASDLRALIEEKANCLAENDLYKLLGVPQQTPPNG
                     IRDAYFALAKQLHPDSIAKAGIDDVRPQAVEVFKGIANAYRTLTDPRKKAEYDAVWAR
                     AAGVSLTPTQAKTKKDLEAEARMFFHKGNLMVKRMQWEEAIAAFRKAVELDERNSEYL
                     VSLGWSVMQNEKLPKTRRLEEARGWFERAISSENPTASSHYYMSLYYKAVGDVPKQRL
                     ELQDALTIDPGHIGAQRERRLLNMRQQNENKADLFAAVKRWWQAQTGPSKPKK"
     gene            2343..3089
                     /locus_tag="PKH54_03465"
     CDS             2343..3089
                     /locus_tag="PKH54_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOC98976.1"
                     /translation="MPMNRITLALVASFAFLLPGCRDMRGSDGQRCGGPLGMCPAVLP
                     APVDPLAPADPGDSVSMIMKELKKGPQNDYIISLQPEDTLNLLAEWSGQTVEELLARN
                     PLVKQTGLIPGNGFTIRLSAGAWNTFNGARNGFRARLLEKRSRGLEVLDVVRHRVKAG
                     DTMESIARDYGTMIDLVEKFNPKARGLSLYPGDEIMVPIVAGDEAREATGRKSKTERL
                     PGPPAPNPTPVPVPPSESRQLLVDSPGTIR"
     gene            complement(3110..3895)
                     /locus_tag="PKH54_03470"
     CDS             complement(3110..3895)
                     /locus_tag="PKH54_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98977.1"
                     /translation="MRDVGGFLLLVLIVFTILYVSIRFALPLLLVHAGGLVAFAVVAA
                     VIITRGRLRRVHFKSLLLPGYGAALALAAAVIPAVHLTLLWLAGRREALPVVAAFNWT
                     PPLVFAGWILLAGRAQKKEYYSAGHDLEDRIEECLSRAGLLDLKIEIMATAAETEEKP
                     EAWEVDAGIARPAGEGIRGRIIQAILEMKQLRDEYRRLAARFEASVASIRLSGRRDSG
                     IDTGALKTAWAELDGAWIAISRETTLILDEKVGYTCREWEQTA"
     gene            4074..5276
                     /gene="rocD"
                     /locus_tag="PKH54_03475"
     CDS             4074..5276
                     /gene="rocD"
                     /locus_tag="PKH54_03475"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOC98978.1"
                     /translation="MDSKVLMELADTYGARNYKPLPVVIDKAEGVWVFDVEGNKYLDF
                     LSCYSAVSHGHRHQALVDAAIRQIGRVTVTSRAFHNSELGPFMKELCEFTGMEMALPM
                     NSGAEAVETAIKAVRKWAYVVKGVPKYDAEIIVCSDNFHGRTTTVVSFSTEELYKEAF
                     GPFTPGFKVIPFGDADALEKAITPNTAAFLVEPIQGEAGVVLPPDGFLARAAEICRSN
                     NVLFVADEIQTGFGRTGRRFAVEHEGVKPDLMCLGKALGGGVYPVSAVVGSRDVLGLF
                     VPGEHGSTFGGNPVACAVGRAALKVMLDEDLTGKSAENGQYLIEKLASIKSKHIKQIR
                     GRGLFVGMVLHPEAGGARRFCEALMKRGLLCKETHDNVIRFAPPLVITREQIDWAISE
                     VTEVMTTL"
     gene            complement(5453..6595)
                     /locus_tag="PKH54_03480"
     CDS             complement(5453..6595)
                     /locus_tag="PKH54_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98979.1"
                     /translation="MKHVVFLTAAMVVALVATPALASDSGPFSFLQDEGSATAPTQDA
                     APAEPAAEAPAPTDTASAEAAPKKDSAVPDAVAVLTGGTASQERFGGAFSFSQSLGLG
                     SFVMDKFARQPYYGWSINLAPRVFLYKDLWLSMSFSMSGELSQSFTSNNTYPRVFMPS
                     DLFISLKYKYVIPKIKVNFSPFIRIGAPTSAESRARDLYLSTGIGFDLNRMFGKHVLL
                     GYSFRYNKNWNGSTSTVIPRYASLIRLNGAEDAGGGQAYGGGLTSEMSIMNRLMASFI
                     INDQWSISVVLGISNAWTYKGALPSERDEFTNENAKPGRGQSDSTSGVIDISYQPWEH
                     VGFSIGMSSSQPAFTADNKSLRFPFFDFTSEGNNFTQFYFDVTVNY"
     gene            complement(6695..7258)
                     /gene="folK"
                     /locus_tag="PKH54_03485"
     CDS             complement(6695..7258)
                     /gene="folK"
                     /locus_tag="PKH54_03485"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOC98980.1"
                     /translation="MHHEVSIERQYAGFRKVLLGLGSNLGDRRANLTAALDAIDRHPD
                     CRVDMVSSIFRTCPVDAGGGEFLNAAARISTTLQPMELLAFLKQIEAGLGRTGSGRDA
                     RPIDLDILFFEGVRMADGGLTIPHPRWRERQFVLIPLAEVCDSFPDPDEGVPLASLVA
                     RLAMPSPDVVPDAPGKWWKAPGETNDN"
     gene            7336..8601
                     /locus_tag="PKH54_03490"
     CDS             7336..8601
                     /locus_tag="PKH54_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOC98981.1"
                     /translation="MYSGSNCRVKDLGLAPEGRMKIEWAEAHMPVMMALREEYSRTRP
                     LEGLKIAGCLHVTKETAVLMRTLRAAGAELSWSGCNPLSTQDEIAAALADEGFSIYAW
                     RGLTVDEFYWCIEKTLEFGPDLSLDDGADLIFTLHNKHPEYCPKMIGGTEETTTGVHR
                     LRAMGRAGKLLYPVIAVNDAQTKWDFDNVYGTGQSSIDGILRATSVLLAGKVFVIAGY
                     GHCGKGLAMRAKGQGCQVVVTEVDPLQGLRATLDGCRVMPMDEAASIGDIFITATGMK
                     DVVRTRHFESMKDGAIVCNTGHYDCEINIEELEALKASSREIRPNTDEYLMKDGRRIY
                     LLARGRLVNLAAAEGHPSEVMDMSFANQFLSMVRLAREGRSLSRDVHDIPCQQDQDIA
                     SLKLKTMGISIDALTPEQIRYRDDFSAGT"
     gene            8659..>8814
                     /locus_tag="PKH54_03495"
     CDS             8659..>8814
                     /locus_tag="PKH54_03495"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807628.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOC98982.1"
                     /translation="MAKNIKPRNEDYSEWYLNVIAAAQLADYAPVRGCMVIRPTGYAI
                     WESMQKEL"
BASE COUNT         1738 a         2500 c         2801 g         1775 t
ORIGIN      
        1 ctggccctgc ctgacaaatg tcatttcatt aacatccagg aaggcatcga tatcgcggca
       61 tcgcggggcc tgtcgcaggt tcttgtctcc cagggtgtct ataacgagtc gatcgtactg
      121 cacgacggtg tgggtgtttt cggcaggtat gacagttcaa gaaactggga acgcgcgcca
      181 tcgaaggttt cgcagattcg gggcggaacc cttgcggtta ccgctgacgg aatcgcttca
      241 ccaaccatcc tccagggtct tgtcattacc agcgagtcgg catcggcacc agggtcgagt
      301 tcgattgccg tggcggcaag gaacggcagc gggctttcaa tccaggcatg taccgtccag
      361 gcaggcgccg gtgccccagg ggccgccggt gcggccggaa atgcgggcca ggcgggaagc
      421 aacggttcat caggcagcgc cggttgcgaa tacgtgtgtg tattccagat aggcgacgaa
      481 aactgcttcg acatgtgtgg ccagtgcgaa cgtccgcttg gtggtgccgg gggtgccggc
      541 gccgcgggcg cgaatggcgg gaaaggcggg gatggcggtc cgtatgcgac ggccggcggc
      601 gcgggtggct ccggtcttgg caatggcgcc ggaaccggcg gttcgggtgg cccgatgacc
      661 cagaacggaa gccggggcca tgacgggctg gacggcgttt ccggttcgaa cggcatcggc
      721 ggcggggcgt ccggagtctt ctcagcgggc ggctacgccg cggcggacgg ctcgcccgga
      781 acggacggca cggacggtgg cggcggtggc ggtggcggag ccggtggcgg cgacaatccg
      841 cagatctggg agttgaacct ttgcggaacc tacggttcca gcggcggtgg cggcggcggt
      901 ggcggcggcg gcggaaaggg tggtatcggt ggcggtggcg gtggtgcctc ggtcggcatc
      961 cttgcatcgg ctggttcagt cgcgatcgag cagaccatga tccttacggg aagcggcggt
     1021 gcgggcggtg ccggcggatt tggcggctcg gccggttccg gtggaacggc gggccttgcc
     1081 ggtgaccacg gggacggaga cggccagggg gtcggtgccg caggcggtgt cggtggcaaa
     1141 ggtggtgccg gtggcgctgg cggtggcggc ggtggcgggc ccagcttcgg catcgtttgc
     1201 gtcgatgcgt cctacgtgtc ctcgggcatg accgtgctga ccggatccgg tggaccaggg
     1261 ggctcttcga cggtcaacac gggcgcttcc ggatcctcgg gacagtcttt cggctgtccg
     1321 taataaatcg ataatcccgc aatatagttt acttttttgg ctttgatggc ccggtctggg
     1381 cctgccacca gcgcttgacc gcggcgaaca gatccgcctt gttttcgttc tgctgccgca
     1441 tgttaaggag tctgcgttcg cgctgggcgc caatgtggcc gggatcgatc gtaagggcgt
     1501 cctgcagttc cagtcgctgc ttcggaacgt cgccgaccgc cttgtaataa agcgacatat
     1561 agtaatgcga actggccgtc ggattttcgg aactgatggc ccgctcgaac cagcctcggg
     1621 cttcctcgag gcgcctggtc ttcgggagct tttcgttctg catgaccgac cagcccagcg
     1681 atacaaggta ttccgaattc cgctcgtcca gttcaacggc cttcctgaac gctgcaatgg
     1741 cttcttccca ctgcatgcgc ttgaccatca ggttgccctt gtggaagaac atccgggcct
     1801 cggcctcaag atcctttttg gtcttcgcct gggtcggagt caggcttacg ccggcagccc
     1861 ttgcccagac cgcgtcgtat tccgccttct tcctgggatc ggtaagggtg cggtatgcgt
     1921 ttgcaattcc cttgaaaact tcaacggcct gggggcggac atcgtctatg ccggccttgg
     1981 cgatcgagtc gggatgaagc tgcttggcaa gcgcgaaata agcgtcccga atgccgttcg
     2041 gcggggtctg ctggggcacg cccaggagct tgtacaaatc gttttctgca aggcagttcg
     2101 ccttttcctc gattaacgcg cgcaagtcgg acgcgtttgc cgaactgtta tcgatatctc
     2161 ccattgcgtc ccctgtcctt gatgccggcc tgcatgccgc gcgacatcga ctgacctgat
     2221 tctacacccc gcgcgggctt gtgcaatcga gaatctttgc ccgatcaggt gcatgagttt
     2281 ggcgttccca ggtggtcgca atagaatcgt cgtcaggcca ttgccaggtt ccgggtgcct
     2341 gtatgccgat gaatcgaatc actttggcgc ttgtggcgtc gtttgcgttc ctgctgcctg
     2401 gctgccgcga catgcgcggc agtgacggtc agcggtgcgg tggtccactc gggatgtgcc
     2461 cggcggttct tccggcgcct gtcgatccgt tggcccccgc ggatcccggc gattcggtca
     2521 gcatgataat gaaggaactg aaaaaggggc cccagaacga ctacatcatt tcgctgcagc
     2581 ccgaagacac gctcaatctg ctggccgaat ggagtggcca gacggtcgag gaactgctgg
     2641 cccgcaatcc gctggtcaag cagaccggcc tgattcccgg aaacggcttc accatccggt
     2701 tgtccgccgg tgcctggaat actttcaacg gggcccgcaa cggcttcagg gcacggctgc
     2761 tggaaaagcg gtcaaggggg ctcgaggttc ttgatgtcgt caggcatcgg gtcaaggccg
     2821 gcgacacgat ggaaagcatc gccagggact atggaacgat gatcgacctg gtcgagaagt
     2881 tcaatcccaa ggccaggggc ctttctttat acccgggtga cgaaatcatg gttccgattg
     2941 tcgcgggtga cgaagcaagg gaagccaccg gtcggaaaag caagacggag aggctgccgg
     3001 gtccgccggc gccgaacccg accccggttc cggtgccacc ttccgaatcc aggcaattgc
     3061 tggttgacag ccccgggacg atacgctgat ccaccgcttc atcggggctt caggccgttt
     3121 gttcccattc cctgcatgta tacccgacct tttcgtcaag aatcagggtc gtttcgcgag
     3181 atatcgcgat ccatgcgcca tcaagctcag cccaggcggt cttcagcgcg cccgtgtcga
     3241 ttccggaatc gcgcctgccg cttaagcgaa tggacgcaac tgatgcctcg aagcgggccg
     3301 caaggcggcg gtactcgtca cggagctgct tcatttccag aatggcctgg ataattctgc
     3361 cacgaatgcc ttccccggca ggccgcgcga ttccggcatc gacttcccac gcttccggtt
     3421 tttcctcggt ctctgcggcg gtcgccatga tctcgatctt caaatccagc agcccggccc
     3481 gtgagaggca ttcctcgatc ctatcttcga ggtcatggcc ggcgctgtag tactctttct
     3541 tctgcgccct tcccgcaagc aggatccatc ccgcgaatac caggggagga gtccagttga
     3601 aggcggcaac gaccggaagc gcctcgcgcc ttcccgcaag ccacagcagc gtcagatgaa
     3661 ccgccgggat cactgcggcc gcaagggcca aagccgctcc atagccggga agaagaagtg
     3721 acttgaagtg aacccgtcgc aggcgacccc tggtgataat cacggcggca acgaccgcga
     3781 atgcgaccag gccgccagca tggacaagca gcaggggaag ggcaaacctg atcgatacgt
     3841 aaaggatcgt gaaaacaatc aggacaagca gaagaaatcc accgacgtcg cgcattgcgc
     3901 ccttccgaaa atcgaccgaa ttattttaca tgttaagtcc ttgcaaaata aaggaatttc
     3961 cagattctca agtcttcttc gccggttctg tgccgaaacc gggttatatt acacggcgtc
     4021 ccgacaaacg gggcgcaaag tcaacttatt atttatgtcc gggaggtttc aaaatggatt
     4081 ccaaggtgct gatggagctt gctgacactt atggcgcgag gaactacaag ccattgccgg
     4141 tcgttatcga caaggctgag ggcgtctggg tattcgatgt cgaagggaac aaatacctcg
     4201 atttcctgtc atgttactcg gccgtcagcc acgggcaccg gcaccaggct ctggttgatg
     4261 cggcaatccg gcagattgga agggtcaccg tcacttccag ggctttccac aattccgagc
     4321 ttggcccgtt catgaaggaa ttgtgcgagt tcaccgggat ggagatggcc cttccgatga
     4381 actccggggc cgaggctgtc gagacggcga ttaaggccgt tcgcaagtgg gcatacgtgg
     4441 tcaagggcgt gccgaagtac gatgcggaaa tcatcgtgtg ttccgacaac ttccacggca
     4501 gaaccacaac ggtagtaagc ttctcgacag aggagcttta caaggaagcc ttcggtccgt
     4561 ttacccccgg cttcaaagtg attccgttcg gcgacgccga tgctctcgag aaggcgatta
     4621 ccccgaacac cgccgctttc ctggtcgagc ccatccaggg cgaggctggc gtcgtccttc
     4681 cgccggacgg attccttgcg cgcgccgcag agatctgccg cagcaacaac gttctgttcg
     4741 tggccgacga gattcagacc ggttttggaa ggaccggccg caggtttgcc gtcgagcacg
     4801 agggcgtgaa gccggatctc atgtgtcttg gcaaggccct gggcggcggt gtctatccgg
     4861 tcagcgccgt tgttggcagt cgcgacgtcc ttggcctttt tgttccaggc gagcacggct
     4921 cgacgttcgg tggcaatccg gtggcatgcg ccgtcggacg ggctgccctg aaggtcatgc
     4981 ttgacgagga tttgacagga aagtccgcgg aaaacggcca atacctgatc gaaaagcttg
     5041 catcaatcaa gtcgaagcac atcaagcaga ttcggggccg tggtctgttt gtcggaatgg
     5101 ttcttcatcc ggaagccggc ggcgcccgtc gtttctgcga ggcgcttatg aagcgaggcc
     5161 tcctttgcaa ggaaacgcac gacaacgtca ttcgcttcgc acccccgctt gtcatcaccc
     5221 gtgaacagat cgactgggcc atctccgaag tcaccgaagt catgaccacc ctgtaggggt
     5281 gaacaaccct tcggaattct cagggcagga aattcgtccg ccgctctcct tgtacgggcg
     5341 gattacaacc gcccgccgcg aacaggcaca gtgcatccgc ccccccgctc cgtacgggcg
     5401 aatgaaaatt cgcccgcctg caatcacgtt ggagccggac ttgtcttgtg tatcagtagt
     5461 tgacggtgac gtcgaagtag aactgcgtga agttgttgcc ttccgacgtg aaatcgaaga
     5521 acgggaagcg cagggacttg ttgtcggccg tgaatgccgg ctgggacgag gacatgccga
     5581 tcgagaagcc gacgtgctcc cacggctggt acgaaatgtc gatcacgccg cttgtcgagt
     5641 cggactgacc gcggccgggc ttcgcgttct cgttggtgaa ttcatcgcgt tcggacggca
     5701 gggcaccctt gtaggtccac gcgttcgaga tgccgagcac gaccgaaatc gaccactgat
     5761 cattaatgat gaacgacgcc atcaggcggt tcattatgga catctctgaa gtaagtccgc
     5821 cgccgtatgc ctggccgccg ccggcatcct ctgccccgtt caggcggata agcgatgcat
     5881 aacgggggat aaccgtgctg gtggaaccgt tccagttctt gttgtaacgg aacgagtatc
     5941 caagcaggac gtgcttgccg aacatccggt tcaggtcgaa accaatgccg gtcgaaagat
     6001 aaaggtcacg cgcgcggctt tccgcggacg tcggcgcgcc gattcgtatg aacggcgaga
     6061 agttcacctt gatcttcgga atcacgtact tgtacttaag tgaaatgaac aggtcgctgg
     6121 gcatgaaaac ccgcgggtac gtgttgttcg acgtgaagga ctgggagagt tcgccggaca
     6181 tcgagaagga catcgacaac cagagatcct tgtaaaggaa aacgcgcggc gccaggttga
     6241 tgctccagcc gtaatagggc tggcgggcaa acttgtccat gacgaaggaa ccaaggccca
     6301 gggactggct gaaactgaag gcgccgccga atctttcctg gctggccgtg ccgccggtca
     6361 ggacggcaac cgcgtccgga acggcggaat ccttcttcgg agcggcctcg gcagaagcag
     6421 tgtcagtggg ggccggagcc tcggcagccg gttcggccgg agcagcgtcc tgggtgggcg
     6481 cggtagccga accctcgtcc tgaaggaacg aaaacggtcc ggaatccgaa gccagggccg
     6541 gcgtggccac aagcgcgaca accatcgccg cagtcaggaa aacaacgtgt ttcatgaata
     6601 acctcacccg ccccgagggc tgaatccggt gaatcatact tggtacgtgc gggcgaggca
     6661 acatgatttg tgaatgattt tcaatcgcgg catttcaatt gtcattcgtt tcgcctggtg
     6721 ctttccacca ctttccggga gcgtccggca ccacgtccgg tgaaggcatc gccagtctgg
     6781 cgaccaatga ggccagcggc acgccttcgt cgggatccgg aaaggaatcg cagacttcgg
     6841 caaggggtat caggacgaac tggcgttccc tccatcgggg atgcggaatg gtaagtccgc
     6901 catccgccat ccgaacccct tcgaagaaaa gtatgtccag gtcgattggg cgcgcatcgc
     6961 gaccgcttcc ggttctgccg aggccggcct cgatttgctt gaggaaggcc agcagttcca
     7021 ttggttgaag cgtggttgag atccttgccg cagcgttaag aaattccccc ccgcccgcgt
     7081 caaccggaca tgtcctgaaa atcgaggaaa ccatatcaac ccggcagtcc ggatgacggt
     7141 cgattgcgtc gagcgcagcg gtcaggtttg cccttctgtc gccgaggttg gaacccagtc
     7201 cgagcaagac cttccggaac ccggcgtact gtcgttcgat tgacacttcg tggtgcatcc
     7261 aataccatca cgcgccggat cgatccgaga ttatgccctg gatatccggg gtgtcaaacc
     7321 gtcgcgaggt gaagcatgta cagcggttca aattgcagag tcaaggatct gggcctggcc
     7381 ccggaaggtc gaatgaagat cgagtgggcc gaggcgcaca tgcccgtcat gatggcgctg
     7441 agggaggagt actcgaggac tcgtccgctt gaaggcctta agatagccgg ttgtctgcac
     7501 gtgaccaagg aaaccgccgt cctgatgcgc acattgcgag ccgccggcgc cgagctttcg
     7561 tggagcggct gcaatcccct ttcgacccag gacgagattg ccgccgcgct ggccgacgag
     7621 gggttctcca tttatgcctg gcgcggactc actgtggacg agttctactg gtgtattgag
     7681 aagactcttg agtttggtcc ggatctgtcg cttgacgacg gggccgatct gattttcacg
     7741 ttgcacaata agcatccgga atactgtccg aagatgattg gtggaaccga ggaaacgacc
     7801 acgggggttc accgcctcag ggccatgggt cgggccggca agcttttgta cccggtcatc
     7861 gccgtgaacg acgcgcagac gaagtgggat tttgacaatg tgtatggcac cggacagtcg
     7921 tcgatcgatg gcatccttcg cgccaccagc gtgctgctgg ccggcaaggt gttcgtgatt
     7981 gccggatatg gccattgcgg gaaggggctc gcgatgcggg cgaaggggca ggggtgccag
     8041 gttgtcgtga ccgaagtcga tcccctgcag ggtcttcgcg cgacacttga cggatgtcgc
     8101 gtgatgccga tggacgaggc cgccagcatc ggggatatct tcattaccgc gacgggaatg
     8161 aaggacgttg taagaacaag gcatttcgag tccatgaagg acggcgcgat cgtctgcaat
     8221 accggtcact acgactgcga gattaacatc gaggaactcg aggcgctcaa ggcctcatcg
     8281 cgggagatcc ggccgaatac ggacgagtac ctgatgaagg atggtcgcag gatttacctg
     8341 cttgccaggg gacgtctggt gaatcttgcc gcggccgagg gacacccgtc cgaggtcatg
     8401 gacatgtcct tcgcgaacca gttcctgagc atggtgcgcc tggccaggga agggagatcc
     8461 ctttcacgcg atgttcacga cattccgtgc cagcaggatc aggatattgc gtccctgaaa
     8521 ctgaagacca tggggatatc gatcgacgcg ctgacgcccg aacagattcg ttatcgcgac
     8581 gatttctcgg ccggaacctg attcgggccg tggccgggag tccttttcaa gaattctgtc
     8641 accaaacgga gaaccctgat ggcgaagaac ataaagccaa ggaatgaaga ttactcggaa
     8701 tggtacctga atgtgatcgc tgcggctcag ctggccgatt acgcgccggt gcgcggctgc
     8761 atggtcatca ggccgaccgg atacgccata tgggagtcga tgcagaagga gctg
//
LOCUS       DAOPPA010000071         8743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9003_length_8743_cov_4.411142, whole genome shotgun sequence.
ACCESSION   DAOPPA010000071 DAOPPA010000000
VERSION     DAOPPA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9003_length_8743_cov_4.411142"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKH54_03500"
     CDS             <1..810
                     /locus_tag="PKH54_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC98983.1"
                     /translation="VFLFMAGERSLALRELSTRARRCVLDEDGLQFLASARAAGMGAR
                     DAMKAASFLRGRIERDTAWLWTAAWPRPWHQAVERASAVFDVDAALIYSVMRIESGFR
                     KSVRSPAGAGGLMQLMPATAATVAGKAFPAAGLKPKASGPDGNVMAGAALLDQLITHF
                     HGNLPLVLAGYNAGSGAGRRFLRRLGSVQADLFVEAVPFERTREYIRLVGGNLAACRH
                     VYGAGAPAEMAFDLPQTTGPFLDMPSGGSRSEGVAVRGGPSRIGAWLRRPQ"
     gene            complement(761..1615)
                     /locus_tag="PKH54_03505"
     CDS             complement(761..1615)
                     /locus_tag="PKH54_03505"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC98984.1"
                     /translation="MHPWLIEDPFRVGTYGIMAFIGFMVAYALFIQELKRKRDFLNGM
                     KPTVIADVTLMLAVFFGILGSKLAFMLFEAPEFSVEMLMWNAGLTWYGGWFLGCGAVV
                     LWFRMRRLNVAMMADLLAPMLAQGYAFGRVGCQLAGDGDFGVPCVPENIDSAICMTYP
                     QGLVPSPCYAGGVEYLVCPTDAIDPFWFPVHPTPIYESITGFMLFGLLWFLRKRIHLN
                     GVLFAIYCLIAGVLRFLVEFIRMPEMRPERFLGLRDAQLIAIAQVLFGAIIIAVVATR
                     HRSAKARP"
     gene            complement(1675..3117)
                     /gene="thyX"
                     /locus_tag="PKH54_03510"
     CDS             complement(1675..3117)
                     /gene="thyX"
                     /locus_tag="PKH54_03510"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOC98985.1"
                     /translation="MAKIVLAGFNIDYEVLQDIRNGGGVPEFVTPETISAAYARISRD
                     PTDVGELRRKSRNEVLKARQSNAAIVFGMGHHSVAEHAVFNFDITDVSRLAIEALESH
                     RLASYTEKSQRYIKLDEDFVLPAEIEQAGAADPFREYMARSFFRYRKLVDALTSSGMD
                     EKLAGEDARYVLPLAVTGQLGMTINARSLEHLIKRLASHPLSEVRELSTAFLDVAGPV
                     APSLIRFVEPGHYDLHRYEAAIEAARALPTIPASDGPMETGPESTVRMIACDPEGDVF
                     ILATLVQGVINRGLGPAIEWVRGLSTEERMKVFESITAGMSIHDPMPREFEHAGMTFE
                     IIMSAAAFGQAKRHRMASITTQPYDPSLGITIPKSVYAASLANFMIEAFDDAHRMVDL
                     LGGPSSALAGYAWLNANRRRCVMTLNLREFYHMSRLREDGHAQWDIRDIASRMSRTVK
                     RAFPVCGSLLGGKDMVRDAIRERNRKAGID"
     gene            3320..4474
                     /gene="alr"
                     /locus_tag="PKH54_03515"
     CDS             3320..4474
                     /gene="alr"
                     /locus_tag="PKH54_03515"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOC98986.1"
                     /translation="MADRLSWCEISTTALARNMLGFRAVTGPRVTLAPVVKANAYGHG
                     LELVAPVFVRAGADVLCVNDLWEAARIRRIGINVPVHVIGRMTPDSAEEAVDLDVSAI
                     VYDRELVSALDSASRRRGQVFRAHLKLETGTNRQGLRLEEASALVEFIDSLQGVRLSG
                     ASMHFADVEDTTDHQFARAQLDRFQSMLDRLGVGGRPGFVRSVSNSAATILWPQAHFE
                     MVRPGISAYGMWPSSETFVTAALSSRNQIDLKPALAWKTTVVQVREVPADEYVGYGRT
                     FRATSPITMAVIPVGYYDGYDRGLGNGAYALVSGRRAQVRGRICMNMAMLDVTGIPDV
                     HPGSEVVLIGKSGQESISAETFASWCNTINYEVTCRISETIPRIPVEVSK"
     gene            4471..5061
                     /locus_tag="PKH54_03520"
     CDS             4471..5061
                     /locus_tag="PKH54_03520"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC98987.1"
                     /translation="MKPVNSERMDPWELKDTRSLAKFGVYEVLRETRMNPRDGREHSF
                     CVQVSRDWATVVPVTASGQVVLVRQFRPASGEVTWEFPGGVVETGEDPAAAVVRELEE
                     ESGFRAGMLVRLGSFRPNPALIRNSLHVFLASGCMESGSRNFDDSEDLDTFVADVNEV
                     DQMVQDGRIDHALMVAAWLTARRRFPGESFNPPGGA"
     gene            complement(5039..6148)
                     /locus_tag="PKH54_03525"
     CDS             complement(5039..6148)
                     /locus_tag="PKH54_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOC98988.1"
                     /translation="MQLSLQSEPDIRAPGDAECLQARLRMIRGATTDKAVAEKRLRRE
                     TQDMISGGWRYWMCRDTRMARRLGPKAPEVLVVQGTPDLSAPLVAVVGPRRPDGYGME
                     MAMFFAESLGRCGVTVVSGGAVGIDTIAHRSALNAGGRTICVPGGGFKRPFPISNVEL
                     FSRLGRDDGFRDSCMISEYSPSESASRHHFPERNRIVAALADAVLVIQAGFGSGAMIT
                     ADLALEMCIPVFAVPADIHYSSSAATNDLLGRGGRPMCRPGDLETVVNLAGSGLSGFN
                     RKPVRRTSGLGSPWISRGASGRSTRHADGSDARVILEAVRAILSETGRAVDQDSICRQ
                     TGLDVARVQAGLLELELGGGVQRLPGALFMPRPGG"
     gene            6285..>8743
                     /locus_tag="PKH54_03530"
     CDS             6285..>8743
                     /locus_tag="PKH54_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98989.1"
                     /translation="MRLRVTFVSCAILLVFAACNEIPVTDLNESYSVQVQVLRDKGKP
                     AKLDVLWVVDDSTSMCQEQQSLADSFKAFLNVFQQYTAIDMRLAVTTTNVCPRDAAGR
                     IRGDFFYRPNTSIESSCVESRRFPCVESDSGVDACAGMKGVPDPDNWECKNATNSGSV
                     PVYVCDAPDGNPGGFIDPFDGYLLESYSSSCVYRCAGPDSNTECAVNFGMPKGCRQGG
                     SVCGNDGPSLDACMSDPSFEDFTDCKVRCRDADCQKVCLDITSNTTRCDESCGEAGAT
                     CESVCQALQREPKCDIVAGALQGEEKCETRCEHNFGPKMAGLCSKMCGAGVTQDACID
                     MCLDPEQGGIANAGFICLMSCSNGYNCQNNCIQEFGVSNYQCVCPGGNCANIGCAREP
                     RWMYCPGTVTFNEGKPTWKDGPKVLDKDEADKRAVEWIQGKWMWPPDWDPEWRNLPTD
                     NSLESKAMRKLMVETVFEHLFTCMTSLYMDQVDCGTQEQGLLAAWMALDKQGENADQA
                     REFLRDDAYLLIIVISDEDDCSSPEGFPDRDGRWFNYAGQQDSTTACACMRDENGCLP
                     SGECDYSKCLDSKNTFSREKCPLYSTSSLVNKLRSLKPDPAQVLFAAIVGDAAPGEGA
                     SPASAEVDDVRGRFFECRCAKGRDTITTSFNYVCSSDYGVAELGQRYIDAVNLFGSGR
                     YGQLANVCSAGGVAPLLEQIAGFVVPLLGQICLPRPLEWDCQGRCENIFDDSAANCTQ
                     VCGVGVAECLKECNAVFEGRPGCAEVCRSAASIEIRKYNGDGTCAVALDEQGNCPILM
                     ETVGDVQGDFSLVRNSPMCPT"
BASE COUNT         1638 a         2586 c         2802 g         1717 t
ORIGIN      
        1 gtctttcttt tcatggccgg cgagcgatcc ctggcgctgc gggagctttc aacaagggcc
       61 cgcaggtgtg tcctggatga agacggattg cagttcctcg cttctgccag ggccgctgga
      121 atgggagcga gggatgcaat gaaggcggca tcgttccttc gcggccgcat cgaaagggac
      181 acggcctggc tctggacggc cgcctggcca aggccatggc accaggccgt ggaaagggct
      241 tccgccgtgt tcgacgtgga tgcggcgctg atctattcgg tgatgcgcat tgaaagcgga
      301 ttcaggaagt cggtgcggtc gccggccggt gccggcgggt tgatgcagtt gatgcccgcg
      361 acggcggcta ccgtggccgg caaggctttc cccgcggccg ggttgaagcc gaaggcctcc
      421 ggcccggacg ggaacgtcat ggccggggcg gcgttgcttg atcagttgat aacgcatttt
      481 catggcaacc tgcccctggt tctggccggc tacaatgccg ggtcgggcgc cgggagaagg
      541 ttcctcaggc gccttggctc ggttcaggcc gatttgttcg tcgaggcggt tcccttcgaa
      601 aggacgcggg aatacataag gcttgtcgga ggaaatcttg ccgcctgcag gcacgtttac
      661 ggcgcgggtg ccccggcaga aatggcgttc gacctgccgc agacgaccgg gcctttcctg
      721 gacatgccgt ccggcggttc aaggagtgaa ggcgtggctg tcaggggcgg gccttcgcgg
      781 atcggtgcct ggttgcgacg accgcaatga tgatggcacc gaaaagcacc tgggcgattg
      841 cgatgagctg ggcgtcacgg agtcccagga atcgttcggg acgcatctcc ggcatcctga
      901 tgaactcgac gaggaaacgc aggacgcccg caatcaggca gtagatggcg aaaagcaccc
      961 cgttcaggtg aatccttttt cgaaggaacc acagcagccc gaacagcatg aatccggtga
     1021 ttgactcgta gattggcgtc gggtgcaccg ggaaccagaa cggatcgatc gcgtccgtcg
     1081 ggcagaccag gtattcgacg ccgccggcgt aacagggcga aggcaccagt ccctgcggat
     1141 aggtcatgca gatggcggaa tcgatgtttt ccggcacgca ggggacgccg aagtcgccat
     1201 cacccgcaag ctggcatccg acacggccga acgcatatcc ctgcgcgagc atcggggcga
     1261 gcaggtcggc catcatcgcc acgttcaggc ggcgcatccg gaaccacaga accaccgcgc
     1321 cgcatccgag aaaccacccg ccgtaccagg taaggcccgc gttccacatc agcatctcga
     1381 cgctgaactc cggcgcctcg aaaagcatga acgccagctt cgaacccagg attccgaaga
     1441 acaccgccag catcagcgtc acgtcggcga tgacggtggg cttcatcccg ttcaggaagt
     1501 cgcgcttgcg tttcaattcc tggatgaaga gcgcgtacgc caccatgaag ccgatgaaag
     1561 ccatgatgcc gtacgtgccg acgcggaagg gatcttcaat cagccaggga tgcattggaa
     1621 actccattca cgggcgtttc ccgtccgtcc gtcacggccc ggcgcggccg acattcagtc
     1681 gatgccggcc ttgcggtttc tttcgcggat ggcgtcgcga accatgtcct ttccgcccag
     1741 aagcgagccg cacaccggga atgcacgctt gaccgtgcgg gacatcctgg atgcgatgtc
     1801 gcggatgtcc cactgggcgt ggccatcctc gcgaagcctg ctcatgtggt aaaactcgcg
     1861 caggttcagc gtcatgacgc aacgacgcct gttggcgttc agccaggcat agccggccag
     1921 ggccgagctg gggccgccca gcagatccac catgcggtgc gcgtcgtcaa acgcctcgat
     1981 catgaagttt gccaggctgg ccgcatagac ggacttcgga atcgtgattc ccagcgacgg
     2041 gtcgtacggc tgcgtggtga tgcttgccat ccggtgacgc ttggcctgtc cgaaggccgc
     2101 cgccgacatg atgatctcga aagtcattcc ggcgtgctcg aactcacgcg gcatcggatc
     2161 gtggattgac atgccggcgg tgattgactc gaagaccttc atgcgttctt cggtcgaaag
     2221 gccgcgaacc cattcaatgg ccggcccaag accacgattg atgacaccct gcaccagggt
     2281 ggccaggatg aagacgtcgc cttccggatc gcatgcgatc atccgcacgg tcgactccgg
     2341 accggtctcc atcgggccgt ccgacgccgg gatggtcgga agcgcgcgcg cggcctcgat
     2401 tgcggcctcg tacctgtgca ggtcgtaatg gcccggttcc acgaagcgga tcagcgatgg
     2461 cgcgacaggt ccggcgacgt ccaggaaggc ggtcgaaagc tccctgacct cggaaagcgg
     2521 gtgggacgcc agcctcttta tcaggtgctc gagtgatctt gcgttgatgg tcatcccgag
     2581 ctgtccggtg acggccagcg ggagcacgta cctcgcatcc tcgccggcga gtttctcgtc
     2641 catgccggaa gacgtcagcg catccacgag tttcctgtac ctgaagaagg aacgcgccat
     2701 gtactcgcgg aaggggtcgg cggcgccggc ctgttcgatt tccgcgggca ggacgaaatc
     2761 ctcgtccagt ttgatgtatc tctgggactt ttccgtgtat gacgccagcc ggtgcgactc
     2821 gagtgcctcg atggccagcc tggacacgtc cgtgatgtcg aagttgaaga cggcatgctc
     2881 cgcaaccgag tgatgtccca tgccgaagac gatggcggcg ttcgactgcc tggccttcag
     2941 aacttcattc cttgacttac gtcggagttc gccgacatcg gtgggatcgc gcgagattcg
     3001 ggcgtatgcc gccgagatgg tttccggagt cacgaattcc gggacgccgc cgccgttcct
     3061 tatgtcctgt aagacttcgt aatcaatatt gaatcccgca agaacaattt ttgccataac
     3121 gtaccccagc cccctttccc cggatggccg ccagctggct gccagcatat ggatgaaccc
     3181 ggaaaaagta atatggcacg ccggattttt ccggtcaaac ggccgcaaat ctaaagttgt
     3241 tcaaaggtgg tatattccgc gtgtggcgcc ggccggcgtg gccggcggca ccgatgcgga
     3301 tcccggctgg aggctgcaca tggctgacag gctttcctgg tgcgagatat cgacgacggc
     3361 gcttgcgcgc aacatgcttg gtttccgggc ggttaccggt ccaagggtga ccctggcccc
     3421 ggtcgtgaag gcaaatgcct acggtcacgg gcttgaactg gtcgcgcccg ttttcgtaag
     3481 ggcgggggcc gacgtgcttt gcgtcaatga cctctgggag gcggcgcgga tcaggcggat
     3541 cggtataaac gtgcctgtcc acgtcatcgg aagaatgacg ccggattcgg ccgaagaggc
     3601 ggtcgacctc gacgtttccg ccatcgtcta tgaccgggaa ctggtttcgg ctctcgattc
     3661 ggcttcgcgc cgacgcggtc aggttttccg ggcccacctg aagctggaaa ccggaacgaa
     3721 tcgtcaggga ttgaggcttg aggaagcatc ggcacttgtc gagtttattg attctttgca
     3781 gggcgtgcgt ctttccgggg cgtcgatgca cttcgccgat gtcgaggaca ccacggatca
     3841 tcagtttgcg cgggcccagt tggatcgttt tcagtcgatg cttgatcggc ttggggtcgg
     3901 tgggcggccg gggttcgtcc ggagcgtttc caattcggcg gccacgattt tgtggcccca
     3961 ggcccacttt gagatggtca ggcccgggat ttccgcctac ggcatgtggc cgtccagcga
     4021 aactttcgtc acggccgcgc tttccagcag gaaccagatt gacctgaagc ccgcgcttgc
     4081 ctggaagacg acggtggtgc aggttcgcga ggtgccggcg gacgagtacg tcggttacgg
     4141 aaggacgttc agggcgactt cgccaatcac gatggcggtg atacctgtcg gatactatga
     4201 cggctacgac cgcggtcttg gcaatggcgc atacgctctg gtctcggggc gtcgggccca
     4261 ggtcagggga cgaatctgca tgaacatggc catgcttgac gtaaccggca tccctgacgt
     4321 gcatccgggc agcgaagtcg tcctgatcgg caaatccggt caggagtcga tatcggcgga
     4381 gacttttgcc tcctggtgca acaccatcaa ctatgaagtc acgtgccgga tttcggaaac
     4441 cattccaagg attcccgtag aggtgtcgaa gtgaagccag tcaattcgga aaggatggat
     4501 ccctgggaac tgaaggatac gaggtcgctt gcgaagttcg gcgtttacga ggttctgcgc
     4561 gaaaccagga tgaacccgcg cgacgggcgg gaacacagtt tctgcgtgca ggtcagccgt
     4621 gactgggcaa ccgtggttcc ggtcaccgcc tcggggcagg tcgtcctggt gcggcagttc
     4681 cggcccgctt cgggggaagt aacgtgggaa tttccgggcg gcgtggttga aaccggggaa
     4741 gaccctgcgg cggccgtcgt tcgtgaactg gaagaggaat caggtttcag ggcgggcatg
     4801 ctggtgcgcc tgggctcgtt ccggccaaac ccggcgctga ttcgcaacag cctgcatgta
     4861 ttccttgcgt ccggctgcat ggagtcggga tcgcggaact tcgatgacag cgaggatctg
     4921 gacacctttg tcgccgacgt gaacgaggtg gatcagatgg ttcaggatgg acggattgac
     4981 cacgccttga tggttgcagc ctggctgacg gcccgtcgca ggtttccagg cgaaagcttc
     5041 aaccccccgg gcggggcatg aacagcgccc cgggaaggcg ttgaacgccg ccgcccagtt
     5101 caagctcaag cagaccggcc tggacgcggg cgacgtcgag tccggtctgt cggcagatgg
     5161 agtcctgatc gaccgcgcgt ccggtttccg aaaggatggc cctgaccgct tcgagtatca
     5221 cccgggcgtc cgaaccgtcc gcgtgcctgg tcgatcggcc cgatgcgccg cgggaaatcc
     5281 atggcgaacc aagccccgaa gtcctgcgca ccggcttccg gttgaatccc gacagtccgc
     5341 tgccggcaag gttgacgacc gtctccaggt cgccaggtct gcacatcggc cgtccgccac
     5401 gtccaagcag gtcgttcgtc gcggccgacg aactgtagtg gatgtcggcc ggaacggcga
     5461 agaccgggat acacatttcc agcgcaaggt cggccgtaat catcgccccg gatccgaagc
     5521 ccgcctgtat gaccagcacg gcatccgcaa gggccgcaac aatccggttc ctttcaggga
     5581 aatggtgcct ggatgcgctt tccgatggcg aatattcgga tatcatgcag gaatcccgga
     5641 atccatcgtc gcgaccgagg cgcgagaaca gttcgacgtt cgaaatcggg aagggacgtt
     5701 tgaatccgcc gcccggcacg cagatggtgc ggccgcccgc gttgagtgcc gatctgtgcg
     5761 cgatggtgtc gatccccacc gctccgccgc ttacgaccgt gaccccgcat cttccaagcg
     5821 attcggcaaa aaacatcgcc atctccatcc catagccgtc cggccgtctg gggccgacaa
     5881 ccgcgacaag cggcgcagac aggtcggggg tcccctggac gacaagcacc tccggcgcct
     5941 tcgggccaag ccttcgggcc atcctggtgt cgcggcacat ccagtaacgc cagcctcctg
     6001 aaatcatgtc ctgcgtttcc ctgcgcaggc gcttttccgc cactgccttg tcagtcgtgg
     6061 cgccgcgtat catccggagc ctggcctgaa ggcattcggc gtctcccggg gcccggatat
     6121 cgggctccga ctgaagggaa agctgcattt cgtcctcctg atgtccggag actcgaatat
     6181 cgtatcgcga cgccgttcgt ttcaagcgta tcggactgca aggattgcgt gcggttaatg
     6241 tgctgtgata cacttcgggc ctgagtattt tcgggagtgt cgggatgaga cttcgcgtaa
     6301 ccttcgtctc ctgtgcgata ttgctggtct ttgcggcctg caacgagatt ccggtgaccg
     6361 atctgaacga gtcgtattcg gttcaggtcc aggtccttag ggacaaggga aagccggcca
     6421 agcttgacgt cctgtgggtc gttgacgact cgacgtccat gtgccaggag cagcagagtc
     6481 ttgcggacag cttcaaggca ttcctgaacg ttttccagca gtacaccgcg attgacatga
     6541 ggctcgccgt cacgacgacc aacgtatgcc cgcgcgatgc tgccggaaga attcgtggcg
     6601 actttttcta tcgcccgaat acttccatcg aatcgtcctg tgtcgaaagc cgtcgtttcc
     6661 cgtgcgtcga atccgattcc ggggtcgatg cgtgtgccgg catgaagggc gttcccgatc
     6721 ccgataactg ggaatgcaag aacgcgacca attccggttc ggttcccgtt tatgtctgtg
     6781 acgcccccga tggcaatccc ggcggtttca tcgatccttt cgatggttac ctgctcgaat
     6841 cgtacagctc gtcctgtgtc tatcgttgtg ccggtccgga cagcaacacg gaatgcgccg
     6901 tcaatttcgg catgccgaag ggttgcaggc agggtggttc cgtatgtggt aacgacggtc
     6961 ccagtcttga cgcctgcatg agcgatcctt ccttcgagga cttcaccgac tgcaaggttc
     7021 gctgccgcga cgccgactgc cagaaggtct gtctcgacat cacctcgaac accacaaggt
     7081 gcgatgaaag ctgcggcgag gccggcgcga cctgcgagtc ggtttgccag gccctccaga
     7141 gggagcccaa gtgcgatatc gtggccggtg cccttcaggg cgaggaaaag tgtgaaaccc
     7201 gttgcgaaca caatttcggc ccgaagatgg cgggactgtg ctcgaagatg tgcggcgccg
     7261 gggttaccca ggacgcatgc atcgacatgt gccttgatcc ggaacagggc ggcatcgcca
     7321 acgccggttt catctgcctg atgtcgtgca gcaacggtta taactgccag aacaactgca
     7381 tccaggaatt cggcgtttcc aattaccagt gcgtctgccc cggcggcaac tgcgcgaaca
     7441 taggctgcgc gcgcgaaccg aggtggatgt actgcccggg cacggtgacc ttcaatgaag
     7501 gcaagccgac atggaaggac ggaccgaagg ttcttgacaa ggacgaggcc gacaagcgtg
     7561 ccgtcgaatg gattcagggc aagtggatgt ggccgccgga ttgggatcct gagtggcgca
     7621 acctgccgac agacaacagc ctggaatcca aggccatgcg gaagctcatg gttgaaacgg
     7681 tgttcgagca ccttttcacg tgcatgacgt ctctttacat ggatcaggtc gattgcggaa
     7741 cccaggagca gggtctcctt gccgcgtgga tggccctgga caagcagggt gaaaatgccg
     7801 accaggcccg tgagttcctg cgcgacgacg cgtatctgct gatcatcgtg atttccgacg
     7861 aggacgactg ttcgtcgccc gaaggattcc cggacaggga tggcaggtgg ttcaactacg
     7921 ccggtcagca ggacagcacg accgcctgcg cctgcatgcg tgacgagaac ggctgcctgc
     7981 cctcgggcga gtgcgattat tccaagtgcc ttgattcaaa gaacacgttc agccgtgaaa
     8041 agtgtccgct gtattcgact tcgtcgctgg tcaacaagct tcgttcactg aagcccgatc
     8101 cggcccaggt cctgttcgct gcgatcgtcg gagacgccgc cccaggcgag ggcgcatcgc
     8161 ccgcttcggc cgaggttgac gacgtcaggg gtcggttctt tgaatgtcgc tgcgccaagg
     8221 gccgcgatac catcacgact tcgttcaact acgtctgctc ttcggattac ggcgtggccg
     8281 aactcggaca gcggtacatc gatgcggtca atcttttcgg cagcggccgt tacggacagc
     8341 tcgcaaacgt gtgttccgcg ggcggcgtcg cgccactgct tgaacagatt gcgggattcg
     8401 tcgttccgct gcttggccag atctgcctgc cgcgtcccct cgagtgggac tgccagggac
     8461 gctgcgagaa cattttcgac gactcggccg ccaactgcac ccaggtttgc ggcgtcggcg
     8521 tcgcggaatg cctgaaggaa tgcaacgcgg tgttcgaggg tcggccgggg tgtgccgagg
     8581 tgtgccgttc ggccgcgtcc atcgagatcc gaaagtacaa cggcgacggg acgtgcgcgg
     8641 tcgcgctgga cgaacagggc aactgcccca tcctgatgga aacggttggc gatgttcagg
     8701 gcgacttcag cctggttcgc aactcgccga tgtgcccgac gtt
//
LOCUS       DAOPPA010000072         8727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9024_length_8727_cov_4.383764, whole genome shotgun sequence.
ACCESSION   DAOPPA010000072 DAOPPA010000000
VERSION     DAOPPA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9024_length_8727_cov_4.383764"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PKH54_03535"
     CDS             <1..1350
                     /locus_tag="PKH54_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98990.1"
                     /translation="DYCELGQCLTGNPKNCSDNNECTTDSCRAADGECLHVPAPGISC
                     GASNKCIVSGVCNSSGLCDAVFKTCNDNNPCTTDSCNPTTGICIFTPNTLPCNDGNAC
                     TADDKCFNGVCGGTTITCNDNIACTTDTCNQSTGCVFSPVDSACNDNNACTTDSCSAT
                     LGCVNTALPDVPATSCNDNNPCTLSDICKGGICTGTQKDCADTLKCTRDFCNVSNGQC
                     VNEYFVGPCNDDNPCTTNDLCNENAICSGSPVDCNDGIPCTTDYCQSGVGCVNQPNDS
                     ACDDRNSCTTNKCVAGSGCVFSPVTTPIACEDGNPCTVSDTCSTTTGTCAGLPKDCSD
                     GNVCTKDSCNTSNGQCSWSAASGPCDDLIACTTGDTCISGICFGTASDALCDDSNACT
                     SDACSTTQKKCIYQFSGASACDDGDPCTDIDMCVDVLCSGTLNCQLPGCSDDPRCAG"
     gene            complement(1400..3202)
                     /locus_tag="PKH54_03540"
     CDS             complement(1400..3202)
                     /locus_tag="PKH54_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC98991.1"
                     /translation="MDRVLPFEVSPASARVALTSRHRPRNCVWELTLDCNLNCVHCGS
                     SAGRARHAELSTEEALDVVRQLRDLGCELVTLSGGEPLLRPDWFEIARAIADSGMYVN
                     MVTNGLLVDDEVAAKISRSGMCNVGVSIDGPRDVHDRIRAPGSFDKSMAGIRKLVKNG
                     VSVGVMTTINRWNMARLEEIRSLAIDAGASMWRLQLAKPMGEICGDRDFIIAPRHLLD
                     VIPRLAAMKKMGGIQLAVGDSIGYYGPHDATLRGRGWRGRDECWQGCRAGMDAIGIEA
                     DGGVKGCLSIQAKAPDGSDPYREGSVRTRSLADIWFSPDGFAYNRQFSRDQLKGVCRT
                     CSRAARCRGGARCVSTAVSGTVGEDVYCWTRVSQMDSNRRFRDIAANAAAAAGLALIM
                     GSAACNEEDGPGSQDVIVDATQLDGVECDLPSDVPGQDMAVQDSVIPDAGIQDSGDLG
                     DAGQIDCENVCCMCDYGIIPEEVWNECCGTKPLEPMPDAAVNDLDFPDVPQGDAIDCE
                     SVCCECDYGIIPDEVWNECCAVNPPDSSPDAVDTDAIQSDASVDVLECADVCCNCEYG
                     IIPDDVYQECCVSQCKDACCECDYGTPPPPQCCR"
     gene            complement(3279..5120)
                     /locus_tag="PKH54_03545"
     CDS             complement(3279..5120)
                     /locus_tag="PKH54_03545"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOC98992.1"
                     /translation="MFRFGPSVAAIVATLVLGCSGGPGNPDAVEPDDVPGDISGDDVP
                     SVPDTGNPADAFDAGSGSEPGDLPRNDGQEVAIPDDGSSEDVPVLPELVLTRTVVPVV
                     DAGLSLNHALEATGGQPPYSDWTIVKGSLPAGVELDAETGIISGVPSERGFSWFVVEV
                     GDSAGNRASELFGIRVGDPVSTGPLRERADAYQAVYEARHDWGGFSYNCNTPDDPDGN
                     LRLTTLGDATFQSGQCTMAMAYRDAVERTQQTSAYLKKQVDGWRFFQNLTGVPGLIGR
                     SWGKVDYPWEDNHHRDGFWPDNPDSHSYRAEGDYEGYIWVGDVSRDQASGAVLGVAAA
                     YDLADDPETKATAAAFLTDLVDHVWENNLDFKDRNDKPPRYGNIDGDNFEGWPYPNGL
                     NAACSLAWFRIAAHVAEAEGLDDAGRFRERYQELVSRGYIGYMRDYMWVYDGYNTKHY
                     NTYMAYENLFHLARLEDDPVLGPQIDDIFRDTLWLNLDDNTPNRKGVKEGNPVKTTWY
                     LYSTGDRDPVALWDALWQVVVFPDAPLRDRYVKNSDDPEIVVNPDEPTESLYPLPSNR
                     LQPDMVIWHRSTFLLDGGVDSGEERTGCDYLLPYWMGRYYGWIDAAW"
     gene            complement(5178..7022)
                     /locus_tag="PKH54_03550"
     CDS             complement(5178..7022)
                     /locus_tag="PKH54_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98993.1"
                     /translation="MQNTLFRVFVLEQMMRHHPLPTLLIPLMMMAACGSAGSSPDLAG
                     DVPDVGVPDSIQGDLMPDDPGGPDVPVVSDASDAVEPVDSLDAVDAADSVGDEGAPFD
                     FRVRWNAPAGPWPVCDEVAGTSKTLAAKAAYYDWIGPKLHQEPKSGSHEDTARMYNVD
                     CDGPVPVEIVPDDQLPNCVLRSSENNGLWTSMYVASQCFRYGATGDAEALANALRTLK
                     GTYELMQITGRPGLYAREASDPGIAPIQTCPEDPMEYRPPEEGTRKGNYWVKVDTDGC
                     MLDYHSDSAEWVKHPDVCTDLKYAGRCWKRNVSKDEYSGHAYAAGVCAKVVDDPAVRG
                     LATEILSKIAHHLKDNAYWLTDYDGLETRYGSIHAMSLDHIPGFNAKLALVWVKSAAM
                     ASGEQELIDEYYKCLLQSDGELRCIERETEFPVDYTTFLDELGLKIGCQTNFDNVSMM
                     MLAYLNEIWFEPSKAERARFRALFEQASRGPDTDDRDIWSMKVPFYNFMIAAAEGDEA
                     MTAERRNLIESMVNDGICVLKNFQETQIERDEDTTVLESVCESDRHGPLIDGIVPIED
                     RCPATFEWWGDPSSVEKCSFNPNVADNPAGFLAPYWMGRYFGFISEDM"
     gene            7233..>8727
                     /locus_tag="PKH54_03555"
     CDS             7233..>8727
                     /locus_tag="PKH54_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOC98994.1"
                     /translation="MLKNFEKRFEHFFQAIAAASSRRPLVFVGIGLALCALSLYLLSQ
                     MPFLTSRTALLDRTSPEVERFEHYMENFGAASDLIIAIEGIERPELEKYAAELASRLE
                     ELPEVSAADSRLDLNFFVSHAYTILPHEQFDKAATFMDGLLSMDGKMPSSLDEALDAA
                     NRYFEEPPAIPMGEIDMDSARASLNGMQFFLDEWLRWLEPDSKATRIAWEKMLADQPE
                     ARKMVTGNGFYDSHDGSMLFVFVRKAIGSEEFTAIKPFYENVRSTNDALKAEWKAAGR
                     AVPTTGLAGMPATIYEEFSYIQKGVTWTIAGAGILILLIITFVMRSFRRGLVVFGAMG
                     FGSVWAFSMMYFTVGHMNMVTTAFTAVLFGLGVDYGVFISSRIIEEMKRGLPLMDSIR
                     VGTGASARPVVTSGLASMMVFGALGTVEFTGFSELGIVALCGVATVQISTFTLLPALF
                     ALLKPRPAEALGQDTGMEGKDDPGKKIPKPIAAAIVTVAVILAAVGAY"
BASE COUNT         1803 a         2722 c         2488 g         1714 t
ORIGIN      
        1 gattactgcg aactgggcca gtgcctgaca ggcaacccga agaactgcag cgacaacaac
       61 gaatgcacca cggactcatg ccgtgctgcc gacggagaat gcctgcacgt gccggcgccc
      121 gggatttcgt gcggcgcgtc caacaagtgc atcgtaagcg gcgtctgtaa ctcgtcaggc
      181 ttgtgcgatg cggttttcaa gacgtgcaac gacaacaatc cgtgcacgac cgactcctgc
      241 aatccgacga ccggaatatg catcttcacg cctaacaccc tgccgtgcaa cgacggcaac
      301 gcatgcactg ccgatgacaa gtgcttcaac ggggtctgcg gcggcacgac gatcacctgc
      361 aacgacaaca tcgcctgcac caccgatacg tgcaaccagt caacgggatg cgtcttctcg
      421 ccggtcgatt cggcttgcaa cgacaacaac gcctgcacca ccgactcgtg cagcgccacg
      481 cttggatgcg tcaacaccgc ccttcccgat gttccggcca cttcctgcaa cgacaacaac
      541 ccttgcaccc tttcagatat ctgcaaaggt ggaatctgca cgggaacgca gaaggactgc
      601 gccgacacgc ttaagtgcac gagagatttc tgcaatgttt caaacggcca gtgcgtgaac
      661 gaatacttcg tggggccatg caacgacgac aatccgtgca cgaccaacga cctgtgcaat
      721 gaaaacgcga tctgcagcgg cagcccggtc gattgcaacg acggcatccc atgcacgacc
      781 gattactgcc agtccggtgt aggctgcgtc aaccagccga acgactccgc gtgcgacgac
      841 aggaattcgt gcaccaccaa caagtgcgtc gccggaagcg gatgcgtctt ttcgccggtg
      901 acgacgccga ttgcctgtga ggacggtaac ccctgcactg tttcggacac gtgcagcacg
      961 acgaccggaa catgcgccgg actgccgaag gactgcagcg acgggaacgt ctgcacgaag
     1021 gattcctgca acacatcgaa cggccagtgt tcatggtcgg ccgcgagtgg accatgcgac
     1081 gatctgattg cgtgcaccac gggggacacc tgcatttccg ggatctgctt cggaacggca
     1141 agcgacgccc tttgcgacga ctcgaacgca tgtaccagcg acgcatgttc aacgacgcag
     1201 aagaagtgca tctatcagtt ttccggggca agcgcctgcg acgacggcga tccttgcacg
     1261 gacatcgaca tgtgcgtcga tgtactctgc tcgggaacct tgaactgcca gcttccggga
     1321 tgctccgacg acccgcgttg cgccggatag tccacggcca ctttcaatta cgccgacaac
     1381 gcatccataa cgactgattt cagcggcagc actggggtgg cggaggggtt ccgtaatcac
     1441 attcgcagca ggcatccttg cactggctca cgcagcattc ctgataaacg tcgtccggta
     1501 tgattccgta ttcgcagttg cagcagacgt cggcacattc aagcacatcg acggaagcgt
     1561 cggactggat tgcatcggta tcgaccgcat ccggcgagga atccggcgga ttgaccgcgc
     1621 agcattcgtt ccagacctcg tccgggatta tcccgtaatc gcactcgcag cagacgcttt
     1681 cgcaatcgat ggcatcgccc tgcggcacat ccggaaaatc cagatcgttg accgccgcat
     1741 ccggcatggg ctcaagcggc ttggtgccac agcactcgtt ccagacctct tcgggaataa
     1801 tcccgtagtc acacatgcag cagacgtttt cacagtcaat ctgacctgca tcgccgagat
     1861 cacctgaatc ctgaatgccg gcatccggga ttaccgagtc ctgaaccgcc atgtcctgac
     1921 cgggcacatc ggaaggaagg tcacattcga ccccatcaag ctgcgtcgca tcgacgatca
     1981 cgtcctgact gccggggcca tcttcctcgt tgcaagcggc ggaacccatt atcagggcaa
     2041 gcccggccgc ggccgcggca ttggccgcga tgtccctgaa gcgccggttg gaatccatct
     2101 gggaaaccct tgtccagcag taaacgtcct cgccgacagt tccggacacc gcagttgaaa
     2161 cgcaccttgc gccaccgcga cacctggcgg cccgggaaca tgtccggcag acacctttca
     2221 actgatcccg cgagaattgc ctgttgtacg caaagccgtc cggactgaac cagatgtcgg
     2281 ccagggatct tgttcgaacc gagccctcgc ggtaaggatc gcttccatcg ggggccttcg
     2341 cctggatgga aagacaaccc tttacgccac cgtcggcttc gatgccgatt gcgtccattc
     2401 cggcacggca cccctgccag cattcatcgc gaccgcgcca gccgcgaccg cgaagcgtcg
     2461 cgtcatgggg gccgtagtaa ccgatggaat ccccgacggc caattgtatc ccgcccatct
     2521 tcttcatggc ggcaagcctt ggaatgacgt cgagcagatg ccgtggggcg atgatgaaat
     2581 ccctgtcgcc gcatatttcg cccatcggct tggccagttg cagacgccac atcgaagcgc
     2641 ccgcatcaat cgcaagcgag cgtatctcct cgagcctggc catgttccag cggttgattg
     2701 tggtcatcac gccgacgctg acgccgtttt tcaccagctt ccggatgccg gccatcgact
     2761 tgtcgaacga tccgggggcg cggatcctgt cgtgcacgtc acgcggcccg tcgatgctta
     2821 cgccaacgtt gcacatcccg gaacgcgata tcttcgcggc gacctcgtcg tcaaccagca
     2881 ggccgttggt gaccatgttg acgtacattc cggaatcggc aatcgcgcgg gcgatttcaa
     2941 accagtcagg ccgcagaagc ggctcgccgc cggaaagcgt caccagttcg cagcccagat
     3001 cccgaagctg ccgaaccacg tcgagggcct cttcggtcga aagctcggcg tggcgtgccc
     3061 tgccggccga cgaaccgcaa tgaacgcagt tcaggttgca gtcaagggtc agttcccaga
     3121 cgcagttgcg tggccgatga cgtgaagtca gcgcgacacg ggcggaagcc ggagacactt
     3181 cgaaaggcag gactctgtcc atgggcgacc ccttgtattg taaagaatcg gttcattata
     3241 catgtgcgtt gaagcgggat ccatgctggg gcgtgccatc accaggccgc atcgatccag
     3301 ccgtagtaac gccccatcca gtaaggcaga aggtaatcgc aaccggttct ttcctcgccc
     3361 gaatcgactc cgccgtccag aaggaacgtg ctgcggtgcc agatgaccat gtcgggctga
     3421 agccggtttg aaggaagcgg gtaaagggat tccgtcggct catccgggtt gaccacgatt
     3481 tcagggtcgt cgctgttttt cacgtaacgg tcgcgaagcg gcgcgtccgg gaagacgacc
     3541 acctgccaga gcgcatccca gagggcaacc ggatcccgat ccccggtcga atacaggtac
     3601 catgtggtct tgaccggatt tccttccttc acgcccttgc ggttcggagt gttgtcgtcc
     3661 aggttcagcc acagcgtgtc ccggaagatg tcgtcgatct gggggccaag aacggggtcg
     3721 tcttcaagac gtgccagatg gaaaagattt tcatacgcca tgtaagtgtt gtagtgcttc
     3781 gtgttgtatc cgtcataaac ccacatgtag tcgcgcatgt atccgatata cccgcgagac
     3841 accagttcct ggtacctttc gcggaaccgg cccgcatcgt cgagcccttc agcctcggcc
     3901 acgtgtgccg cgatcctgaa ccatgcaagc gagcacgccg cgttcaggcc gtttggatac
     3961 ggccagcctt cgaagttgtc gccgtcgatg ttcccgtacc ttggcggctt gtcgttcctg
     4021 tccttgaaat cgagattgtt ttcccagaca tgatcgacca gatcggtaag aaaggcggcc
     4081 gcggtcgcct tcgtctccgg gtcgtcggcc aggtcgtacg cggccgcgac accgagcacg
     4141 gcgccgcttg cctgatcccg actgacatcg ccaacccata tgtacccttc gtaatcgcct
     4201 tcggcgcggt aactgtgcga atccgggttg tccggccaga atccgtcacg gtggtggttg
     4261 tcttcccacg gataatcgac cttgccccag cttcttccga taagccccgg aacacccgtc
     4321 aggttctgga aaaaccgcca gccgtcaacc tgcttcttca ggtaagccga agtctgctgg
     4381 gtacgttcga ccgcgtccct gtaagccatc gccatcgtgc actgaccaga ctggaaagtc
     4441 gcgtcgccaa gggtagtcag gcgcaggttg ccgtccggat cgtcgggcgt gttgcagttg
     4501 tagctgaagc cgccccagtc gtgcctggct tcatagacgg cctggtatgc gtccgccctt
     4561 tcgcgcagcg ggcctgtcga aacgggatcc ccgacccgga tgccgaagag ctcggaggcg
     4621 cggttgccgg ccgagtcacc aacctcgacg acaaaccagg aaaaccccct ttccgaaggc
     4681 acccctgaaa tgatccccgt ttcggcatcc agttcgacgc cggcaggcag gctccccttc
     4741 actatggtcc agtctgaata aggcggctgg ccacccgtcg cttcgagggc atggttcaat
     4801 gacaagccgg catccacgac gggcacgact gtccttgtaa gaaccagctc gggcagaacc
     4861 gggacatctt cagatgagcc atcatcaggg atggcgactt cctggccgtc atttcgcgga
     4921 agatcgcccg gctccgaacc cgaaccagca tcgaacgcat ccgctggatt accggtgtcg
     4981 ggaaccgaag gaacgtcatc gccggaaatg tcgcccggga catcgtccgg ttcaaccgca
     5041 tccgggtttc caggcccgcc ggaacagcca aggacaaggg tagctacaat cgcggcaacc
     5101 gacgggccaa acctgaacat tggtacctcc agcgtcattg aatgccagga cggacacgcg
     5161 ccggaaaaag gccggcgtca catgtcttcc gaaatgaacc cgaagtatcg ccccatccag
     5221 taaggggcaa ggaagccggc cgggttgtcg gcgacgttcg ggttgaagct gcacttttca
     5281 accgatgaag ggtcgcccca ccattcaaac gtcgccggac agcgatcctc gattggaact
     5341 atgccgtcaa tcaacggacc gtggcggtcg gactcgcaca ccgattcaag cacggtggtg
     5401 tcttcatctc gctcgatctg ggtttcctgg aaattcttga ggacgcagat tccatcgttg
     5461 accatggact cgataaggtt ccttcgttcc gcggtcatcg cttcatcgcc ttcggccgcg
     5521 gcgatcatga aattgtaaaa gggcaccttc atcgaccaga tgtccctgtc atcggtatcc
     5581 gggcctcgac tggcctgttc gaacagggca cggaaacgcg cacgctccgc cttcgacggc
     5641 tcgaaccaga tctcgttcag gtacgcaagc atcatcatgc tgacgttgtc gaagttggtc
     5701 tggcaaccaa tcttcaaccc gagttcgtcc aggaaggtgg tgtaatcgac cgggaattcg
     5761 gtctcccttt cgatacaccg cagttcgccg tcggactgga gaaggcactt atagtattcg
     5821 tcaatcagtt cctgctcgcc tgatgccatc gcggccgact tgacccagac aagcgccagc
     5881 ttcgcgttga aacccgggat gtggtcaagg gacatggcat ggatcgaacc gtacctggtc
     5941 tcgagaccgt cgtaatctgt cagccagtag gcattgtcct taagatgatg cgcaatcttc
     6001 gaaaggattt cggtcgccag cccgcgaacg gcgggatcat cgacaacctt ggcgcacacg
     6061 ccggccgcgt atgcatggcc gctgtattca tccttggaca cgttgcgctt ccagcagcgc
     6121 ccggcatatt tgaggtccgt gcagacatcc gggtgcttca cccattccgc cgagtccgag
     6181 tggtaatcca gcatgcaccc gtccgtatcg accttcaccc agtaattgcc cttcctggtg
     6241 ccttcctcgg gcggtcggta ctccatcggg tcttcgggac aagtctggat tggggcgatc
     6301 ccgggatccg aagcctcgcg ggcgtaaaga ccgggacgcc cggtgatctg catcaactcg
     6361 tacgtgccct tcagggtgcg cagcgcattt gcaagtgctt ccgcatcccc ggtcgcgccg
     6421 tacctgaagc actgggacgc aacgtacatc gacgtccaca gcccgttgtt ctcgctgctg
     6481 cgaagaacac agttcggcaa ctggtcgtcc ggcacgattt ccaccggtac cgggccgtca
     6541 caatccacgt tgtacatcct tgcggtgtct tcgtgcgatc cggacttcgg ttcctggtgc
     6601 agcttcggcc cgatccagtc gtaataggcg gccttggcgg ccagcgtctt cgatgttcca
     6661 gccacctcgt cgcagaccgg ccacggaccg gccggcgcat tccaccgaac cctgaagtcg
     6721 aacggagcgc cctcatcccc gaccgaatcg gcggcatcga ccgcgtcaag tgaatcgacc
     6781 ggctcgacgg catcggatgc gtcggaaacg accggaacgt ccggcccgcc gggatcgtcc
     6841 ggcatcaagt ctccctggat cgaatcgggc acgcccacgt cggggacatc cccggccaga
     6901 tccgggcttg aaccggcgga accgcaggcg gccatcatca tcaggggaat cagaagcgtt
     6961 ggaagggggt gatgtcgcat catctgctcc aggacaaaga cccggaagag ggtattttgc
     7021 atgccgacgc cagtcaacaa gtgatttcgg ggactaacat ggtcagggtc gatatggcct
     7081 ttgatggcgc tgaccggact gggggtgcct tccttgactt tcgaccgcca gaacggtgac
     7141 aatctgcacc gaattccgac ctgccggatc ggttcaatga acacggcagg tcgcacaacc
     7201 atttgaaatt attaactcgc ccaggtctta acatgttgaa gaatttcgaa aagcgattcg
     7261 agcatttttt ccaggccatt gccgcggcct cctcgagacg tccacttgta tttgtgggta
     7321 taggcctggc cctgtgcgcg ctgtccctct atctgctgag ccagatgcct ttccttacat
     7381 cacgcacggc gctgctggac agaaccagcc cggaagttga acgtttcgaa cactacatgg
     7441 aaaacttcgg cgccgcctcg gacctgataa tcgcaatcga aggcatcgaa cggccggaac
     7501 tggagaagta cgccgcggaa ctggcgtccc gacttgaaga gcttcccgag gtgtcggccg
     7561 ccgacagccg tcttgacctg aactttttcg tttcccatgc atacaccatc cttccccacg
     7621 agcagttcga caaggctgcg acattcatgg acggactcct gtcgatggat ggaaagatgc
     7681 cgtcaagcct ggacgaggcg cttgacgcgg ccaaccgcta tttcgaggag cccccggcca
     7741 tcccgatggg cgaaatcgac atggattcgg ccagagcttc actgaacggc atgcagttct
     7801 tcctggacga atggctgcgc tggcttgaac cggacagcaa ggcgaccagg atcgcgtggg
     7861 aaaagatgct ggccgatcag ccggaagcac gcaagatggt caccggcaac ggcttctacg
     7921 attcccacga cggttcgatg cttttcgtgt tcgtacgaaa ggccatcggc tccgaggaat
     7981 tcaccgcaat caagcctttc tatgagaacg taagatcaac caacgacgcg ctgaaggcgg
     8041 aatggaaggc ggccggacgc gcggttccga cgacgggcct tgccggaatg cctgccacaa
     8101 tctacgagga attcagctac atccagaagg gcgtcacctg gacaatcgcc ggcgcaggca
     8161 tattgatcct tctgatcatc actttcgtca tgcgttcgtt ccgccgcgga ctggtcgtat
     8221 tcggcgccat gggcttcggg tcggtctggg ccttctcgat gatgtacttc accgtcggac
     8281 acatgaacat ggttaccacc gccttcacgg cggtgctgtt cgggcttggc gttgactacg
     8341 gggttttcat ctcatcacgg atcatcgagg agatgaagcg cggactgccg ctgatggact
     8401 ccatccgcgt cggaaccggg gcttcggccc gaccggtcgt gacttccgga cttgcttcga
     8461 tgatggtgtt cggggcactt ggaaccgttg aattcaccgg attttcagaa ctgggaatcg
     8521 ttgccctgtg cggcgtggca accgtccaga tttcgacgtt caccctgctt ccagcgctgt
     8581 tcgcgctgct gaagccgagg cctgccgagg cactcggaca ggacaccggc atggaaggaa
     8641 aggacgaccc gggaaagaaa atcccgaagc cgatcgcggc ggcaatcgtc acggtggccg
     8701 tcattctcgc cgcagtcggt gcctaca
//
LOCUS       DAOPPA010000073         8665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9099_length_8665_cov_4.363531, whole genome shotgun sequence.
ACCESSION   DAOPPA010000073 DAOPPA010000000
VERSION     DAOPPA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9099_length_8665_cov_4.363531"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PKH54_03560"
     CDS             <1..1302
                     /locus_tag="PKH54_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC98995.1"
                     /translation="GDCGTGYSCQPVGADKHCLRDCIDDGDCLGGYICYASGIQKSCL
                     PITYNCVPCSFDSPCKDGQCCDFNTGQCKDCRGVCFTCTYDYDCAAGLRCFKQWGSGN
                     GFCVEECGETECTDTAHFQCNDNGKGVDICQPTTEGCGGCAEPTPFPFEGGCVDCRNN
                     DDCADNEMCNTLEHKCIPSTCGGDTIMCKADMKCHQCCTDDDCLRFPEATGVCLEDGT
                     CDGVVPCGGLCTPDFPICTVVQGVEQCVQCVTDADCAMINAGCTCVGDPLFSCIDETG
                     AICQTPGVFCQATCEDSGDCPPTSTGGAMECAPISGGTTGFCYDPSGSCDGSSSCCAP
                     GMKCWDLILLLMSGMMGGMGGMPIDPSMMSSRSGYCECNVAEDCLNGAPCADLSLVCA
                     LVSLGGGGGSGGILDIIVTFASLLCPGGQLAAGMPGHLCMH"
     gene            1449..2438
                     /locus_tag="PKH54_03565"
     CDS             1449..2438
                     /locus_tag="PKH54_03565"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC98996.1"
                     /translation="MHSNFNILGMDFPAYFTLLMAGYTFVILLGHHDWVSRGRRNNVI
                     LDLGIILILAGLLGARLMHVAADGHFMDYVNHCRDPLKVEGELLPGERKCATAAECVA
                     AKKGELCHPEKGTCHQERDCLRAFKFWYGGLTFYGGLGLAALAGIIYIRKKRQPLWEI
                     GDLAGYAIPLGLVFGRIGCFLAGCCFGTTCASPPGVSFPRGSPAWQLHVDGGLIGKAA
                     AGSLPVHPTQLYEAAACLLIFLWLYFIVRPRKRFSGQLFFLFCMSYAVARFAVEFLRA
                     DDRGEMFGLSTSQFVGIPLFLIGLVLYLKNRGRGIDMTGIEPELSEAEDDGVR"
     gene            2422..4449
                     /locus_tag="PKH54_03570"
     CDS             2422..4449
                     /locus_tag="PKH54_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HOC98997.1"
                     /translation="MMASGDQGTVVLARSDSGPVAGVVVESRAGKLGVVTVAGVRESW
                     VASRVFFFSGVRISSGSAQHAAAAVEAFVQRISVMAESADLAGAWELLGGRADVGADE
                     LADLLFSADGSEEQAAAAWAVEKDSIHFKMIAAGRYAPASEAAVTSQLRERAASAREA
                     EVLDRVHRAIAGLLDSGRAAVMSDHDVAAGVGWLSSLVYDGPEGRDGRRGIGLLGMLR
                     GASPSEPGMAAFETLVRLGVCDPDEIVSIRANRIDAPFDPAVEKEAGRIVAAVAECSR
                     QETFSWQAGHGPIAIDDESTKDVDDALMVKAIEGGFEVHVLIADPASFVTLDSHVGRA
                     GLARASSLYAPTGTFPMLPRVLSEGALSLSADGGRPMLDFSVVLAADGNVIDFRIVPA
                     YGRIESRMTYDGADLMLSGQATGADERLRILAMLAEALRLRRVAAGAVMLEKDEYHVK
                     VIDGVVSVRRVPYASPSRRMVAEFMILACSLAGRFARNNGIPVVYRRQDPPDRDAAER
                     LTGLQPGSRAWYYNMLRTLKRGELSTVPGYHHSLGVAGYTQVTSPLRRFQDLLAHVQI
                     KGFLTDGKWPIDADRLMNLFAELESRVEIVTRVERESRRYWILRYLDGFCGRQVEGEV
                     VARIGGRVLVELDDTGLVLPVSAGAAQPGARLRLVVRDVDPRRDSATLTPA"
     gene            4517..7072
                     /locus_tag="PKH54_03575"
     CDS             4517..7072
                     /locus_tag="PKH54_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOC98998.1"
                     /translation="MEHKFCEANRRDMAMHPWISFLHMVRQPSRYVGQEFGSCANESA
                     PNSIALVFPDVYEVGMSHLGTQILYSILSARPDIRVERAFSPWPDLETELRSRKLPLV
                     SLETWSPLGGFDVLGFSLQHELCYTNVLAALELSEVPLMASDRGDGDPVILAGGPCAS
                     HAEPVAPFFDAMFIGEAEGAIGDLVGLVGGLRRAGTPRQGILEAMSRVPGVYVPSLYR
                     TEPDRSTGWLVPVPAGRSVPERVSRVFVPDLDAYRIPARSIMPWNRAVFDRVSVEVAR
                     GCSEGCRFCEAGFSYRPLRDRDPAGVLKDSLDAIAFCGYEELSLGALSPADYPALPGL
                     VSALSSCATPRNVTLSVSSLRAYGLSDSVLKDMKAVRTAGLTLAPEAGTQRLRDIINK
                     NVTEDDMISAARRAFSAGWQRLKLYFMIGLPTETDEDVSAIVELAGKVLRMGRGIGRA
                     EVNASVGVFVPRPHTPFQRLGMATVEQISARQRILREASNRRGVSFKYPDQHVSRLEC
                     AMARGDRRLADVVIRAYRAGCRFDNWTETFKADEWARAFVDSGIDMQDYLGPVPAGAR
                     LPWDVIDMLIEPGFLESEMERALQGKLTPPCEKPSSGYPEPESFAAGVVCHACGVGCD
                     PRGTAAARGRVAEAGRSLQAPPPWIVPEGTSSWHVGYARAGASAWLSQIDLVKHIPRI
                     FKRAGYEPVFSGGFHPMPRFTYCDPMPVGHQSIGEWLEARIAGTVMPDIGMLNRHSID
                     GLHFVSIGSPIQRKGPPRPPTFAFRCPVDADTAAKLLGELSAGPVADFDSLPGACFLN
                     PSFSKPGKIAFSVVWPSRAARPAEKLHEWISSLFGREFLPVELVRLYDGPAAG"
     gene            7076..>8665
                     /locus_tag="PKH54_03580"
     CDS             7076..>8665
                     /locus_tag="PKH54_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC98999.1"
                     /translation="MNTTHAAWVVILAVSVPLCPLRAEAGNASTAHRDPLMELAHVSA
                     LRRLGFRDAAADALKASTVFRSKKNSRISALVDAAAAGLRGLSAGSARDCGVLPDLPE
                     LAAHVMYERGRELANSGSGVNCLRLFLDRFPDHVDASEARLVVARFLLQEGRNREAAE
                     LAAMVRTGSAGRGLKAMAWLLAAGTLPEPGRTSALTDLFVAMPDTVAASSAGISEDSL
                     DNDALRRRAANFFDAFDFVNYQAALRKLWQRGDRSPELACKIARSHIVHVRDDPEEAL
                     RMMLFARERGVCVDPEGTFLLGRIHAKLEDYGAAEKCFREYLATGAKAKRMLSSYYLA
                     WLPYDHGEYERALPLMDRFLKEYRSSDRYSYMIWFKGWSLFRLKQYREAISVFESMKK
                     LGNNLVAGKAMYWGGVALHRLGDHKGARVWMNEVISRYPLTWYSVLAAKRLKQWYRTP
                     LPGWMTAPAALAGDYEPMFDSPRIGRLASEAAKVSAGLFAAGRPAEAWPVWRPHADAA
                     EKRLAGRDRARFMLTMADVTEQ"
BASE COUNT         1445 a         2535 c         2862 g         1823 t
ORIGIN      
        1 ggcgattgcg gaaccggata ctcctgtcag ccggtcggtg ccgacaagca ctgcctgcgc
       61 gactgcatcg acgacggtga ctgcctgggc ggctatatct gttacgcctc cggcatccag
      121 aagagctgcc tgccgattac atacaactgc gtgccgtgca gctttgattc gccctgcaag
      181 gatggtcagt gctgcgactt caacaccggt cagtgcaagg attgtcgcgg cgtgtgcttc
      241 acctgcacat acgattacga ctgcgcggcc ggcctgcgct gcttcaagca gtggggaagc
      301 ggcaacggtt tctgcgtcga ggaatgcggc gagacggaat gcaccgacac ggcccacttc
      361 cagtgcaacg acaacggcaa gggcgtcgat atctgccagc ccacgaccga aggctgcggc
      421 ggctgcgccg agccgacccc gttcccgttc gagggtggct gcgtcgattg ccgcaacaac
      481 gacgactgcg ccgacaacga aatgtgcaac actctcgaac acaagtgcat ccccagcacc
      541 tgcggcggcg ataccatcat gtgcaaagcc gacatgaagt gtcatcagtg ctgcaccgac
      601 gacgactgcc tgcgcttccc ggaagcgacc ggcgtgtgcc ttgaagacgg cacgtgcgac
      661 ggcgtggttc cgtgcggcgg cctgtgcacc cccgacttcc cgatctgcac cgttgtccag
      721 ggcgttgaac agtgcgttca gtgcgtcacc gacgccgact gcgcgatgat caacgccggc
      781 tgcacctgtg tcggcgaccc gcttttcagc tgcatcgacg aaaccggcgc gatctgccag
      841 acccccggcg tattctgcca ggcgacctgc gaagactccg gcgactgccc tccgacatcg
      901 accggcggag ccatggaatg cgcgccgatc agcggcggca ccaccggctt ctgctatgac
      961 ccgtccggct cctgcgacgg ctcgtcgtcc tgctgtgccc ccggcatgaa gtgctgggat
     1021 ctgattcttc tgctgatgtc cggcatgatg ggcggcatgg gcggcatgcc tatcgatccg
     1081 tcgatgatgt cgagccgttc cggttactgc gaatgcaacg tagccgaaga ctgcctcaac
     1141 ggcgctccct gtgcggatct ttcacttgtt tgcgccctgg tcagtcttgg tggcggcggt
     1201 gggtccggtg ggattctcga catcattgtt acttttgcca gcctcttgtg tcctggtgga
     1261 caactcgcag cgggcatgcc tggacatttg tgcatgcatt gacatcagca gcctgcttcc
     1321 attctgatcc gttttcccct ccgccttctt ccgggataat ccgttccaac cattaatctg
     1381 aacgccagtc cgtcgattcc gccatgctgg tagtacaata ggcgcggtct ttcggatccg
     1441 gggttccgat gcacagcaac ttcaatatcc tgggtatgga ttttccggcc tatttcacgc
     1501 tgctgatggc cggatacacg ttcgtgatcc tgctggggca tcacgactgg gtatcgcgtg
     1561 gacgcaggaa caacgtaatc ctggatctgg gcatcatcct gatccttgcc ggccttctgg
     1621 gtgcaaggct gatgcacgtc gcggccgacg gccacttcat ggactacgtc aatcattgca
     1681 gggatccgct gaaggtcgag ggcgagcttt tgccgggcga aaggaagtgc gcaacggccg
     1741 cggaatgcgt tgccgcgaag aagggcgagc tgtgccatcc agaaaagggc acgtgccacc
     1801 aggaaaggga ctgcctgcgg gcgttcaagt tctggtacgg gggactgacg ttttacgggg
     1861 ggctcgggct ggccgcgctt gcgggcatca tctatatcag gaaaaaaagg cagccgctgt
     1921 gggagatcgg cgatcttgcc ggatatgcga ttccgcttgg cctggtcttc ggccgcatcg
     1981 gctgcttcct tgcgggctgc tgctttggaa caacatgcgc atccccgccc ggggtttcgt
     2041 ttccaagggg cagtccggcg tggcagcttc acgttgacgg cggcctgatc ggaaaggcgg
     2101 cggcgggctc gcttccggtg cacccgaccc agctttacga ggctgcggcc tgtcttttga
     2161 tctttttgtg gctgtacttt atcgtccggc cccggaagcg tttctcgggc cagcttttct
     2221 tcctgttctg catgtcgtac gcggtcgcgc ggtttgccgt tgaattcctg cgcgccgacg
     2281 atcgcggcga gatgttcggc ctttccacat cgcagtttgt tggaattccg ctctttttga
     2341 tcggcctggt gctctatctg aagaaccgcg gtcgcggcat cgacatgacg gggatcgaac
     2401 cggagttgtc ggaagcggag gatgatggcg tcaggtgatc agggaaccgt cgtcctggcc
     2461 aggtccgatt ccgggccggt cgcgggcgtg gtcgtcgaat ccagggcggg caagctcggc
     2521 gtcgtgactg tcgcgggtgt ccgcgagtca tgggtcgcgt cacgcgtttt ctttttttcg
     2581 ggcgtcagga tttcgtcagg ttcggcccag catgcggcgg cggctgtcga ggcgttcgtg
     2641 cagcggataa gtgtcatggc cgaatcggcc gaccttgccg gcgcgtggga actgcttgga
     2701 ggcagggccg atgtcggggc cgacgaactg gccgatctgc tcttttccgc cgacggatcg
     2761 gaggagcagg ccgccgcagc ctgggcggtc gagaaggatt cgattcattt caagatgatt
     2821 gccgcgggca ggtatgcgcc cgcttcagag gccgcggtga cttcgcagct tcgcgagagg
     2881 gccgcaagcg ccagggaagc cgaagttctt gacagggttc atcgggcgat tgccggactg
     2941 cttgattccg gacgggcggc cgtcatgtcg gatcacgacg tggccgcagg cgtgggctgg
     3001 ctttccagtc ttgtttacga cgggccggag ggccgcgatg gtcgaagggg aatcgggctg
     3061 ctcgggatgc ttcgcggcgc cagcccctcc gaacccggga tggccgcatt cgagacgctt
     3121 gtgcggcttg gcgtctgcga tcccgacgag attgtctcga ttcgtgcaaa ccggattgac
     3181 gcgccgttcg atccggccgt cgagaaggaa gccggaagga ttgtcgcggc ggttgccgag
     3241 tgttcgcggc aggaaacctt ttcgtggcaa gccggccacg gtccgattgc gattgatgac
     3301 gaatcaacga aggacgtcga tgacgccttg atggtcaagg cgatcgaagg cggtttcgaa
     3361 gtgcatgtgc tgattgcgga tccggcgtct tttgtaacgc ttgattccca cgtaggccgg
     3421 gcggggcttg caagggcgtc cagcctttac gcgccgacag gcaccttccc gatgctgccc
     3481 cgtgtccttt ccgagggggc gctttccctg tccgccgacg gcggtcggcc gatgctcgat
     3541 ttttctgtcg ttctggcggc cgacggcaat gtcatcgact tccggattgt tccggcttac
     3601 ggccgaatcg agtccaggat gacctacgac ggcgcggatc tgatgctgtc aggccaggcg
     3661 accggggccg acgaacggct caggatattg gcgatgctgg ccgaagccct ccgcttgcgg
     3721 cgcgtggccg caggcgcggt gatgcttgaa aaggacgagt accacgtcaa ggtcatcgat
     3781 ggcgtggtgt cggtccggcg cgttccgtac gcgtcgccgt caaggcgcat ggtagctgag
     3841 ttcatgatac ttgcgtgttc ccttgccggc cgtttcgcgc ggaacaacgg cattccggtt
     3901 gtctacaggc gccaggatcc gcccgacagg gacgcggccg agcgcctgac gggcctgcag
     3961 ccgggatccc gggcctggta ctacaacatg ctcagaacct tgaagcgcgg cgagctttca
     4021 acggttccgg gttatcatca ttcgctcggg gtcgccggct acacgcaggt gacctcgccg
     4081 ctgaggcgtt tccaggactt gctggcccat gtccagatca agggttttct tacggatggt
     4141 aaatggccga tcgacgcgga tcgcctgatg aacctttttg cggaactgga atcacgcgtt
     4201 gagattgtca caagggtgga acgcgagtct cgaagatact ggatcctgag atatcttgac
     4261 ggtttctgcg gccgccaggt cgagggcgag gtggttgccc ggattggcgg cagggtactg
     4321 gtggaacttg acgatacggg tctggtgctg ccggtttcag ccggcgcggc ccagcctggg
     4381 gcgcggctgc gcctggtggt gcgcgacgtc gatccccgtc gggacagtgc gacattgact
     4441 ccggcatgac aggacgctcg gcggggcttt cgtcccggtt ccgttccgtg gccggcgcaa
     4501 catgagagag gttttcttgg aacacaagtt ttgtgaagcg aatcgtcgcg acatggcgat
     4561 gcacccgtgg atatcgtttc ttcacatggt caggcagccg tcgcggtatg tcggacagga
     4621 attcgggtcg tgcgcgaacg aatcggcccc caactcgatc gcccttgtct ttcccgacgt
     4681 gtatgaagtc gggatgtccc acctgggaac gcagattctt tattcaatcc tttcggcgcg
     4741 cccggatatc agggtcgaaa gggccttttc accctggccg gatctcgaga ccgaactgag
     4801 gtctcgaaaa cttccgctgg tgtccctgga gacctggagt ccgcttggcg gattcgacgt
     4861 gcttggattc tcgcttcagc acgaactttg ttacacgaat gtccttgcag ccctggaact
     4921 gtcggaagtc ccgctgatgg cgtcggatcg cggcgatggc gatccggtga tactggccgg
     4981 cggtccctgc gcctcgcacg ccgaaccggt tgccccgttt tttgacgcga tgttcattgg
     5041 cgaggccgaa ggtgcaatcg gtgacctggt cggcctggtc ggcggcctga gaagggccgg
     5101 cacgcccagg caggggatcc ttgaagcgat gtcccgcgtt cccggcgttt atgttccatc
     5161 gctgtaccgg acggaaccgg atcggtcaac gggatggctc gtgcccgttc cggcaggccg
     5221 ttcggttccc gaaagggtca gccgcgtgtt tgtgccggat cttgacgctt acaggattcc
     5281 tgccaggtcg atcatgccgt ggaacagggc agtattcgat cgggtgtcgg tcgaggtcgc
     5341 ccgcggctgt tcggaaggct gcaggttctg cgaagccggt ttttcatacc ggccgctgcg
     5401 cgaccgagat ccggccgggg tcttgaagga cagccttgat gcgatcgcgt tctgcggtta
     5461 cgaggagctt tcactcggcg cgctttctcc ggcggattac ccggcgctgc ccgggctggt
     5521 atccgcgttg tcatcttgcg cgacgcccag aaacgtcacg ctctcggttt cctctcttcg
     5581 cgcgtatggt ctttccgaca gcgtccttaa agacatgaag gccgtcagga cggcaggact
     5641 gacccttgcg ccggaagccg ggacgcagcg gttgcgcgac ataatcaaca agaacgtcac
     5701 cgaggatgac atgatttcgg ccgcccggcg ggccttttcg gccggctggc agcggctgaa
     5761 gctttatttc atgatcggtc tgccgaccga gacggacgag gacgtctcgg caatcgtcga
     5821 gcttgccggc aaggttctga ggatggggcg cggcatcggc agggcagagg tcaacgcgtc
     5881 cgtgggcgtt ttcgtgccaa ggccgcacac tcctttccag cggctgggaa tggcgaccgt
     5941 cgaacagatt tccgcccgcc agcggatcct tcgggaagca tcgaaccgcc ggggcgtatc
     6001 cttcaagtat ccggatcagc atgtcagcag gcttgaatgc gcgatggcga ggggcgaccg
     6061 ccgccttgcc gacgtggtga ttcgtgccta ccgggccggc tgcaggttcg acaactggac
     6121 tgaaaccttc aaggccgatg agtgggcgcg cgcgttcgtg gattcgggca tcgacatgca
     6181 ggattacctt ggcccggttc cggccggggc gcgccttccc tgggacgtga ttgacatgct
     6241 gatcgaacct ggattccttg aaagcgaaat ggaacgggcg cttcagggaa agttgacgcc
     6301 accctgcgag aagccgtcat cggggtatcc tgaaccggag tcttttgctg ccggggttgt
     6361 ctgccatgcc tgcggcgttg gctgcgaccc gcgcggcacc gcggcggcaa ggggccgcgt
     6421 cgccgaggcc ggccggtcgt tgcaggctcc gcccccctgg atcgtgcctg aagggacgtc
     6481 ttcctggcat gtcggatatg cgcgtgccgg ggcctcggca tggctttcgc agatcgatct
     6541 ggtcaagcac ataccgcgca tcttcaagcg cgccggatac gagccggtct tttccggcgg
     6601 attccatccg atgcctcgtt tcacctattg cgatccgatg ccggtcggtc accagtcaat
     6661 cggcgagtgg cttgaggcca ggattgccgg caccgtgatg cctgacatcg ggatgctcaa
     6721 ccggcattcg atcgacggcc ttcattttgt atccatcggt tcgccgatcc agcggaaggg
     6781 cccgccacgg ccgccgacgt tcgcgttcag gtgtccggtt gacgctgaca ccgcagcgaa
     6841 actgctgggc gagctttccg caggcccggt ggcggacttt gacagcctgc caggggcgtg
     6901 tttcctgaac ccgtcgtttt cgaagccggg gaaaatcgca ttttcggtcg tctggccatc
     6961 aagggcggcc aggccggccg agaagcttca tgaatggatt tcttccctgt ttggccgcga
     7021 attcctgccg gtcgaactgg taaggctgta tgacggaccg gcggccgggt gattgatgaa
     7081 tacgacgcac gcggcatggg tggtgatcct ggcggtatcg gtgccgcttt gcccgcttcg
     7141 ggccgaagcc ggtaatgcct cgaccgcaca ccgcgatcca ttgatggaac tggctcatgt
     7201 ttctgcattg aggcggcttg gctttcggga cgcggcggca gatgcgctca aggcttcgac
     7261 cgtgttcagg tcgaaaaaga atagtcgaat atccgccctg gttgatgccg cagcggcagg
     7321 acttcgcggt ctttcagccg gaagcgcccg cgattgcggc gtcctgccgg atctgcccga
     7381 actggcggcc catgtcatgt atgaaagggg cagggagttg gccaattccg gatcgggcgt
     7441 gaactgcctg cggctttttc tggatcgttt tcctgaccac gtcgatgcgt ccgaggccag
     7501 gctcgtggtt gccaggttcc ttctgcagga ggggcgaaat cgggaggccg ccgaactggc
     7561 cgccatggtg cggacaggtt cggccgggcg cggcctgaag gccatggcgt ggctgttggc
     7621 cgccggcacg cttcccgaac cgggcaggac cagtgcgttg accgatctgt tcgtcgcgat
     7681 gccggacacg gtcgcagctt caagtgccgg gatttccgaa gattcccttg acaacgacgc
     7741 gctgcgaagg cgcgcggcga atttcttcga tgccttcgat ttcgtcaatt accaggcggc
     7801 tcttcggaag ctctggcagc gtggcgacag atctcctgaa ctggcgtgca aaatcgcgag
     7861 atcccacatc gtccatgtgc gcgacgaccc ggaagaggcg ttgcggatga tgctctttgc
     7921 gagggagcgt ggcgtgtgcg ttgatccgga gggaaccttc ctgcttggac gcattcacgc
     7981 gaagctggag gactatggcg ccgccgaaaa atgcttcagg gagtatcttg cgaccggcgc
     8041 gaaggccaag cggatgcttt cgtcgtatta tcttgcctgg ctgccatacg atcacggcga
     8101 atacgaacgc gcgctgcccc tgatggacag gttcctgaag gaatacagga gcagcgaccg
     8161 gtacagctac atgatctggt tcaagggctg gtcgcttttc cggttgaagc aataccgcga
     8221 ggcgatttcc gtctttgaat ccatgaagaa actcgggaac aacctggtgg ccggcaaggc
     8281 catgtactgg ggtggcgttg ccttgcacag gcttggtgat cacaagggcg ccagggtctg
     8341 gatgaacgaa gtgatctcca ggtatcccct gacatggtat tcagtgcttg ccgcaaaaag
     8401 gctcaagcag tggtacagga caccgcttcc cggctggatg actgccccgg ccgccctggc
     8461 tggcgattac gaaccgatgt tcgactcgcc ccggatcggg cgtctggcgt ccgaggcggc
     8521 aaaggtttcc gctggtctgt tcgccgcggg ccgtccggcc gaggcttggc ctgtctggcg
     8581 cccgcacgcg gatgccgcgg agaagcggct ggccggccgc gacagggcca ggttcatgct
     8641 gacgatggcc gatgtgaccg agcag
//
LOCUS       DAOPPA010000074         8654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9110_length_8654_cov_4.333295, whole genome shotgun sequence.
ACCESSION   DAOPPA010000074 DAOPPA010000000
VERSION     DAOPPA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9110_length_8654_cov_4.333295"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKH54_03585"
     CDS             complement(<1..466)
                     /locus_tag="PKH54_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOC99000.1"
                     /translation="MSEATINGNIEDRRARIALVAMLMFSVVGIAIAADLANIYVASR
                     TDPDFQSFCAVSDGINCTSVALSEYSTVFNTPVAVWAIAGYLFTAFLSGMALFRKRTG
                     FGTGFLFLFGCLYTLVSIWLIIVMTFLIHSICILCMAIDVINVAFMVMAIFAI"
     gene            630..2414
                     /locus_tag="PKH54_03590"
     CDS             630..2414
                     /locus_tag="PKH54_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99001.1"
                     /translation="MMCRLHGYCLPLFVLIIAGIVGCVNEKCFENFHCPSPKVCGSEG
                     VCVFECVLPSDCLAGFECVGNVCRPSQPRDVFEAVAPILYCPAGMVMVENSFCIDIYE
                     ASRPDSTSASPGVDGSRAVTRKGAHPWMTTDNQVAAAACAASGKRLCLPAEWGLACSG
                     PDDTVYSYGDSYEPAVCNGIDTYGRSGFHLMPTGSFPGCVNEYGVFDMNGNVWEHVAG
                     GDGMDVRGGAYNCSDSAKLHKCSYIPGNWIPSALGFRCCQDPLELNPDDVERVEMVAD
                     AVATDGGNADSAVVGDIALPDSPVEDICTGPDSDMFQVDDGGTDGATVEPFDSGMQDL
                     VEDGSGDAAAEVFDVAPDPGLFDAAVDAAVDSGIDAAAWSCPDDMVVVLHPSRTPFCM
                     DRYEAARLDSTSSTAGTSNTPVSQPERMPWASSSVNLAVAKAACASVSKRICMQDEWI
                     DACSGSVGSVYAYGNAYDPAVCNSIDTFCYCDTTCSTVLECPYPHCRVRSSPAGDGGP
                     CGAAFHVVATGSFPRCVNEWGAYDINGNVWEVTDAGDGIDHFRGGAYNCSDSEALHRC
                     DHDGNWGPSARGFRCCMDAVPASGGPGR"
     gene            2411..3928
                     /locus_tag="PKH54_03595"
     CDS             2411..3928
                     /locus_tag="PKH54_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99002.1"
                     /translation="MTRFLASVLLAILFFLPGPASSSEGGDGIDRAKAALASNPGDAG
                     LRRELGIAYYRAGNDFWAVKVLSGLVEENAADCATRAWLGLALLRRAFPEQALASVDA
                     DAARACEGPDLMRVLMVRAMALAALGRDADTDAALRQAASSKAGYDGDVKALPGLKRR
                     LAPDSVPDVAWSVEIGGGYTSNARMGSATDSASEDDGGAPMTSVDARVRLSPDTGTIV
                     RPVLEIQPRLNLFLMGPASKFGFFDFTGQVGLLFRNVLPRMFVGYRPEWLVLTHGFKA
                     DDSPVLYTAAHRGEFEVEILPSLMVFGGVGHRTFAEMVKTRIEADIGLGGSVRPVGNL
                     TALWAVAGRYYDARHEAWSQVGMSVSGAFVMRLERDWMARVSASMSFDYYPWSAATIT
                     GDPFGSTANRFDLLVRTGGQLWGPSMSGARIGVSYDFSIRDSTAAAYSFTDHRLLLRL
                     KVTGSADFRRPASVSGDNGASGGIDWGQEGSGLDERVRDMLRQDEQVQRSSSCAN"
     gene            3956..4450
                     /locus_tag="PKH54_03600"
     CDS             3956..4450
                     /locus_tag="PKH54_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOC99003.1"
                     /translation="MTGWKRIVRDAIVVAAASAAVGILFNLAREEGIPLVAESPYEIF
                     VPCPEPLGEVDAVLPTSGLLDQVEIRAGRTLVIDAGSSSDFSSWHLRGAINIPFDYIE
                     PVADQVIRRVASSRARHVVVYGDGADPDSGRELARELAGRGISNIAFVEGGVQALKSA
                     RGAE"
     gene            4447..4944
                     /locus_tag="PKH54_03605"
     CDS             4447..4944
                     /locus_tag="PKH54_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOC99004.1"
                     /translation="MNRLSRTASAVVSCPWLILALRLYIGYVFLAACFHKIVHPAMFA
                     VDVATYDILPLAVINLMAIVLPWIELAAGLMLVAGFRARAAALLTSGMMVVFIVAISI
                     ALARGLHMSCGCFASQGAVEDPISLLTVARDAVWFIMSVWILVFDRNSFGVDHLVERL
                     SAWKV"
     gene            4947..5804
                     /locus_tag="PKH54_03610"
     CDS             4947..5804
                     /locus_tag="PKH54_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC99005.1"
                     /translation="MRMSLTAFLVLIGPVVAVAAPPECDRLSGKAAETAAAVLDSEYM
                     YDCCDQTIRKCMSGAPACVDRAASIAAHVCRLAAGGADRGAIVRSLEKRAMSVTGRRT
                     AQNLAAPPEGAVAGDAGAPVIVLVYTCARCPFCSKLVPQLRAEVETGRLKGVARLVMR
                     PFPIKSHQNSAEANQALAAAIMIGRGWPFLMEAYRRFDSFKVGDIPAIAAAAGMEAAK
                     FQAAASSDASRQALVDSKKEGLRLGVDSTPTIFIDGRKYESELDVETVVDVILEVASG
                     QKGQAGSLK"
     gene            complement(5783..6715)
                     /locus_tag="PKH54_03615"
     CDS             complement(5783..6715)
                     /locus_tag="PKH54_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99006.1"
                     /translation="MEKNRIYGPVPSRRLGFSLGIDLVEYKHCPLDCIYCQLKRTTRL
                     DASRETTVEIEEVVRQVDAKIATGARVDHLTLGGSGEPTLYGQLGGLIDALHERFDIP
                     VALLTNGVLFGDPAVRAEAARADVVLPSLDAWDQESFELVNRPIAGMGFEAFMDGMIR
                     FRQQYRGLIWLEVMLLAGISDNPDVISRLAAQAARLKPDRIQLNTPVRPPATDLAIPI
                     SRQTMTMLADLFKPPAEVTADFPEPAGNDPETHVTTVQVLETITRRPCTARDVAVGLN
                     APEGEVSAALRILVGEGRIVLSVQDGKDYFKLPA"
     gene            6862..8103
                     /locus_tag="PKH54_03620"
     CDS             6862..8103
                     /locus_tag="PKH54_03620"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOC99007.1"
                     /translation="MAEHGGHQSKEHTATAVLKMSEIKHKVLVMSGKGGVGKTTVAVN
                     LAYTFAARGFNVGIVDTDIHGPNVALMTGVEGLPAEGGDGVIEPHQAADRVKVLSISG
                     FLPGPDSPVVWRGPMKSGVIAQFLAEGNWEGIDLIVVDCPPGTGDEPLSVAQLIPDAD
                     GVVVVTQPQDVSLLDSRKCINFVRMLKLPVLGIVENMSGLVCPHCGERIDLFSVGGGR
                     KAAQDMDVPFLGAIPITTKMVGAGDSGRPLVDSEPSDPAAVALDEIASRVEQEWTRVA
                     AERKRARIRTVVVAAASMDGIKAAVSSQFVSAPAFVAVDVDEDEIDDVRTIRNPFADG
                     HQSGQIPRWIHDELGAHVIIAGEIGSHAFDAFNSYGMQIVKGVSGTVADVVKSWMESR
                     MPEGCAPSSCAGCSKDCSSRK"
     gene            8139..>8654
                     /locus_tag="PKH54_03625"
     CDS             8139..>8654
                     /locus_tag="PKH54_03625"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC99008.1"
                     /translation="MWPYVIREPIEIGTYGVMMAVGFLTASWWLGRDLRRRNIDPAVG
                     DMTIMIAIVLGVIGSKLAYLLTEADSFTLKDFVSGAGLTWHGGLILAAAGIIGYWLSK
                     KFPLPVMLDTVSPMLASGYAFGRLGCLISGDGCYGVTCQPSSWDHFMCMAFPNGIVPT
                     APGVVVHPTPLY"
BASE COUNT         1549 a         2421 c         2807 g         1877 t
ORIGIN      
        1 ggatggcgaa gatggccatc accatgaaag ccacgttgat cacgtcgatc gccatgcaaa
       61 ggatgcagat cgagtgaatg aggaaggtca tcacgatgat cagccagatg gaaaccagcg
      121 tgtagaggca accgaacaga aagaggaagc ccgtaccgaa accggttctt ttccggaaga
      181 gcgccatgcc ggacagaaag gccgtgaaca ggtagcccgc gattgcccag acggccaccg
      241 gggtattgaa gaccgttgaa tactcggaca gtgcgaccga cgtgcagtta atcccgtcgc
      301 tgaccgcgca gaagctctgg aaatccggat cggtcctgga cgcgacatag atgttggcca
      361 gatccgcggc gatcgcgatt cccaccacgc tgaacatcag cattgccacc agggcgatcc
      421 tcgcccttcg atcttcaatg tttccgttga tagtagcttc gctcatcccc ggcctctctg
      481 cagaaagtcc acgcacgccg aatttacgga cggattgttc aaaaatgcaa ggtcgtcgcg
      541 atctcgtcgc cggttcggca attgataatc gttttcaaat gtagcattat tcccgacgtc
      601 ttcggcgctg ctgcggtaaa ggaaattcaa tgatgtgccg ccttcatgga tactgtcttc
      661 cgctgttcgt cctgataatt gcggggatcg tcggctgcgt taacgaaaag tgttttgaaa
      721 attttcattg cccatccccg aaagtgtgcg gatcggaagg cgtctgcgta ttcgagtgcg
      781 tgcttccctc ggactgcctg gccggtttcg aatgcgttgg caatgtttgt cgcccgtccc
      841 agccccggga cgtgttcgag gccgtggccc cgattctgta ctgtccggcc ggcatggtca
      901 tggtggaaaa ttctttctgt atagatattt acgaggcttc ccggccggat tccacctcgg
      961 ccagtcccgg ggttgacgga tcccgcgccg tcaccaggaa gggcgcccat ccgtggatga
     1021 caaccgacaa ccaggtcgcg gcggccgcct gcgccgcatc gggaaagcgc ctgtgcctgc
     1081 cggcggagtg gggcctggcg tgttcagggc cggacgatac cgtttattca tatggcgatt
     1141 cgtacgaacc ggctgtctgc aacggaatag acacctatgg aagaagtgga tttcatctga
     1201 tgccgacggg atcttttccg ggatgtgtca acgaatatgg cgtgttcgac atgaatggaa
     1261 acgtctggga acacgtcgcc ggcggagatg gaatggatgt gcggggcggt gcctataact
     1321 gcagcgattc cgccaagctt cacaagtgct cgtatatccc ggggaactgg attcccagcg
     1381 cacttggctt ccgctgctgc caggatccgc tggaactcaa tcccgatgat gttgagcgcg
     1441 tggaaatggt ggccgatgcg gtggcgacgg acggtggaaa tgccgattct gcggttgtcg
     1501 gggacatcgc ccttccggat tcacccgtcg aggacatatg caccggtccg gattcggaca
     1561 tgttccaggt ggacgatggc ggcaccgacg gcgcaaccgt ggaacccttt gacagcggaa
     1621 tgcaggatct ggttgaggat gggtcagggg atgcggccgc cgaggttttc gatgtggcgc
     1681 cggatcccgg attgttcgac gcggcggttg atgcggccgt tgattcgggt attgatgcgg
     1741 ccgcctggtc gtgtcccgat gacatggtcg tcgttcttca tccttcccgg acaccgttct
     1801 gcatggacag gtatgaagcc gccaggctcg attcgacttc atccaccgcc ggcacttcca
     1861 atactcccgt aagccagccc gagcgcatgc cgtgggcgtc gtcatcggtc aacctggcgg
     1921 tcgcgaaggc ggcgtgcgcc agcgtttcaa agagaatctg catgcaggac gaatggattg
     1981 acgcatgttc cggttcggtc ggaagcgtct atgcgtatgg caatgcctac gatccggccg
     2041 tctgcaattc aatcgacacg ttttgttatt gcgacacgac ctgctcgacg gtgctggaat
     2101 gtccctatcc ccactgccgg gtgcgttcct cgcccgccgg ggacggaggg ccgtgcgggg
     2161 ccgcctttca cgtggtcgcg acgggatcct ttccccggtg cgtcaacgag tggggtgcgt
     2221 atgacatcaa cggcaacgtc tgggaggtga ccgacgccgg cgacggcatc gaccacttcc
     2281 gaggcggggc ttataactgt tcggattccg aggccctcca tcgatgtgat catgacggga
     2341 actggggtcc gtccgcccgt ggtttcaggt gttgcatgga tgccgtgcct gcgtcgggag
     2401 ggccaggaag atgacaaggt ttctggcgtc ggtgttgctc gcgatccttt ttttcctgcc
     2461 gggtccagcc agttcatcgg aaggcggcga cggaattgat cgcgcgaaag cggcactggc
     2521 atccaacccc ggggacgcgg gacttcggag ggagcttgga atcgcctact atcgggccgg
     2581 caatgacttc tgggccgtca aggtgctttc gggtcttgtg gaagagaacg cggccgactg
     2641 cgcgacgcgg gcctggcttg gtcttgcgct gctgaggcgt gcattcccgg agcaggcgct
     2701 tgcctctgtc gatgccgatg cggcgcgagc ctgtgaaggc cccgatctga tgagggttct
     2761 gatggtcagg gcgatggccc tcgccgccct tggaagggat gccgacacgg acgcggccct
     2821 gcgtcaggcc gcttcgtcga aggccggtta cgatggagac gtcaaggccc tgcccggctt
     2881 gaaaagacgc cttgccccgg attcggttcc ggatgtcgcg tggtcggtcg agataggtgg
     2941 cggctacacg tccaatgcaa ggatgggatc ggcaaccgat tccgcgtccg aagacgacgg
     3001 gggcgcgccg atgacatcgg ttgacgcaag ggtgcgcctg tcccccgaca ctggcaccat
     3061 cgtccgcccg gttctggaaa ttcaaccgag gctgaatctg ttcctcatgg ggccggcatc
     3121 gaagttcggg tttttcgatt ttaccggcca ggtcggattg ctttttcgaa atgttcttcc
     3181 acgaatgttc gttggataca ggccggagtg gctggttctg acacacggat tcaaggccga
     3241 cgactcgccg gttctttaca ccgcggccca ccggggcgag ttcgaggtgg agatactgcc
     3301 ttcgctgatg gtttttggcg gtgtcggcca tcgaaccttc gcggagatgg tcaagacacg
     3361 aatcgaggcg gatatcgggc tggggggatc cgtgaggccc gtcggcaacc ttacagcgct
     3421 ctgggcggtg gccggcaggt attacgacgc ccggcatgaa gcctggagcc aggtcggcat
     3481 gtctgtttcg ggggctttcg tgatgcggct ggaacgcgac tggatggcca gggtgtcggc
     3541 ttcgatgtca ttcgattact acccctggtc ggcggccaca ataaccgggg atccatttgg
     3601 atccaccgcc aaccggttcg atcttctggt caggacgggc gggcagttgt ggggtccgtc
     3661 gatgtcgggc gcaagaatcg gcgtttccta cgatttttcg attcgtgaca gtaccgccgc
     3721 ggcctattcg ttcacggatc atcgcctgct tctgcggctg aaggtaaccg gcagcgcgga
     3781 cttcaggcgt ccggcgtcgg tgtcgggcga caacggggct tccggcggga tcgactgggg
     3841 ccaggaaggc tctgggctcg acgagcgagt ccgggacatg ctcaggcagg atgaacaggt
     3901 gcagcgttct tcgtcctgcg ccaactgaca cgtgttcgta ttggaggagt gcctgatgac
     3961 gggatggaaa cgaatcgtca gggacgcgat tgtcgtcgcg gcagcgtccg cggccgtggg
     4021 aatcctgttc aacctggcaa gggaggaagg tatccccctg gtggccgaat ccccctacga
     4081 gattttcgtg ccttgtcccg agccgcttgg tgaagtcgat gcggttcttc ccacgtccgg
     4141 gctgcttgac caggtcgaga tccgggcggg acgcacgctg gtgatcgatg ccggaagttc
     4201 atccgatttt tcgtcctggc acctgcgcgg cgcaatcaac atcccgtttg attacattga
     4261 gccggttgcc gaccaggtga tccgccgcgt cgcgtcttca agggcgcgcc atgtggttgt
     4321 atacggcgac ggcgccgacc cggattcggg gcgggaactg gcacgggaac tggcggggcg
     4381 tggcatttca aacattgcct tcgttgaagg gggcgtgcag gcgctgaagt cggcaagggg
     4441 ggcggaatga accggctgtc gcgaaccgct tccgcggtag tttcatgccc atggctgata
     4501 ctggcattgc gcctgtatat cgggtacgtc ttcctggcgg catgctttca caagattgtt
     4561 catcccgcga tgttcgccgt ggatgtcgca acgtatgaca tcctgccgct ggcggtcatc
     4621 aacctgatgg cgatagtcct tccgtggatt gaactcgcgg ccggcctgat gctggtcgcc
     4681 ggcttcaggg ccagggccgc ggcgttgctg acctcgggca tgatggtcgt attcatcgtc
     4741 gcgatttcga ttgcccttgc ccgcgggctg catatgtcct gcggatgttt cgcgtcccag
     4801 ggcgcggtcg aggatccgat ttcgctcctg accgtcgcca gggatgctgt ctggttcatt
     4861 atgtcggtgt ggattctggt ttttgatcgc aactctttcg gggtcgatca cctcgttgaa
     4921 aggttgtcgg catggaaggt gtgataatga gaatgtccct cacggcgttt ctggtgctga
     4981 ttggtccggt cgttgcggtg gcggcgccgc ccgagtgcga ccgtctttcg gggaaggccg
     5041 ccgagacggc ggcggccgtt cttgattctg aatacatgta cgactgttgc gaccagacaa
     5101 tccgaaaatg catgtccggc gccccggcat gcgtggacag ggccgcgtca atcgcggcac
     5161 atgtatgcag gcttgcagcc gggggcgccg acaggggcgc cattgtaaga agcctggaaa
     5221 aaagggccat gtcggttacg gggcggcgca cggcgcagaa ccttgcggca ccgcctgaag
     5281 gggctgtcgc cggggatgcc ggggctccgg tcatcgttct tgtctataca tgcgccaggt
     5341 gtccgttctg ttcgaaactg gtgccccagc tccgggcaga agttgaaacg ggaaggttga
     5401 agggcgttgc caggctggtc atgaggccgt ttccgataaa gagccaccag aattcggccg
     5461 aggccaacca ggctcttgcc gccgcaataa tgatcgggcg gggctggcca ttcctcatgg
     5521 aggcgtaccg gcggttcgat tcgttcaagg tcggtgacat accggccatc gccgcggcgg
     5581 caggcatgga ggccgcgaaa ttccaggctg cggcatcgtc ggacgcttcg aggcaggcgc
     5641 tggtcgattc gaagaaggaa ggattgcgcc tgggtgtcga ttcgaccccg accatcttca
     5701 tcgacggaag gaaatatgag tcggaactcg acgttgaaac tgttgttgac gtaatcctcg
     5761 aggtcgcgtc aggccagaaa ggtcaggccg gcagcttgaa gtagtccttt ccgtcctgta
     5821 ccgacaggac aatcctgcct tcaccgacaa gtatcctgag tgccgccgaa acttcacctt
     5881 ccggtgcgtt caggccgaca gcgacgtccc tggccgtgca cggccggcgg gtgatagttt
     5941 ccagaacctg cactgtcgtg acatgggttt ccgggtcgtt tccggcaggt tcaggaaagt
     6001 cggcggtgac ctccgccggc ggtttgaaaa ggtctgcaag catggtcatc gtctgtcttg
     6061 aaatcgggat cgccaggtcg gtcgcgggcg gcctgaccgg ggtgttcaac tggatccggt
     6121 cgggtttcag ccgcgccgcc tgggcggcaa gacgcgatat gacgtccggg ttgtccgaaa
     6181 ttccggccag cagcatcact tcaagccaga taagtcccct gtattgctgc cggaatctga
     6241 tcatgccgtc catgaaagct tcaaagccca tcccggcaat cggcctgttg accagttcga
     6301 acgattcctg atcccatgcg tccaggctcg gcagcaccac atccgcccgg gccgcctcgg
     6361 ccctgactgc cggatccccg aacaggacgc cgttcgtcag cagggccact gggatgtcga
     6421 atctttcgtg aagcgcatcg atcagtccgc caagctgtcc gtacagcgtc ggttcgcccg
     6481 aaccgccgag cgtcaggtga tctactcttg cgccggttgc gattttcgcg tcgacctgtc
     6541 ttaccacctc ctcgatctcg acggtggttt cgcgactggc gtcaagccgc gtggttcgtt
     6601 tcaactggca gtatatgcag tcgagcgggc agtgcttgta ttcgaccagg tcgatgccca
     6661 ggctgaagcc gaggcgtcgg cttggaaccg gcccgtatat cctgttcttt tccatgtcga
     6721 cctccgccga tccgaggcag cctgaatagt cgacagccgc tcgggatatg tcaagacagg
     6781 ttgaatcatc ggttcataag tggtaactag tcccgacctt ggtcgcgacg cttttcattg
     6841 atttgtccgg gaggtttgaa aatggccgag catggcggtc accaatcgaa ggaacacacc
     6901 gcgacagcgg ttctgaagat gtcggaaatc aagcacaaag tgcttgtaat gagcggaaag
     6961 ggtggtgtcg ggaagaccac ggttgccgtt aacctggcct acacttttgc tgcccgtggc
     7021 ttcaatgtcg gcatcgtcga taccgatatc cacggaccga acgtggcgct catgaccggg
     7081 gtcgagggcc ttccggccga gggcggcgac ggcgtgattg aaccgcacca ggcggccgat
     7141 cgcgtcaagg ttctttcgat ttccggcttt cttcccgggc cggattctcc cgtggtgtgg
     7201 cgtggtccga tgaagtcggg ggtcatcgcc cagttcctgg cagagggcaa ttgggagggc
     7261 atcgatctga tagttgtcga ttgcccgcct ggaaccggcg atgaaccact ttcggtcgca
     7321 cagttgatcc cggacgcgga tggcgtggtc gtcgttaccc agccacagga cgtgtcgctt
     7381 ctggacagca ggaagtgcat caatttcgtg cggatgctta aactgcctgt tctcggcatc
     7441 gtcgagaaca tgtccggcct tgtctgcccg cactgcggcg aaaggataga tcttttttcc
     7501 gtcggcggtg gcaggaaggc ggcgcaggac atggatgtgc cgtttctggg cgcaataccc
     7561 atcacgacga aaatggtcgg cgccggagac tcgggcaggc cactggtcga ttcggaacct
     7621 tcggatcccg ccgcagtcgc gcttgacgag attgcgtcac gtgttgaaca ggaatggacc
     7681 agggttgccg ccgagcgcaa gcgggccagg atccgcaccg tcgtcgttgc ggcggcctca
     7741 atggacggga tcaaggcggc ggtcagctcg cagttcgttt cggcccctgc cttcgtggcg
     7801 gtggatgtcg atgaagacga gatcgatgac gtacggacga ttcggaatcc tttcgccgat
     7861 ggtcatcagt ccggccagat accgcgctgg attcacgacg aacttggtgc ccacgtgatc
     7921 atcgccggcg aaatcgggtc gcacgccttc gatgccttca acagctacgg aatgcagata
     7981 gtcaagggtg tttccggcac ggtggccgac gtggtcaagt cgtggatgga aagccgcatg
     8041 ccggaaggtt gcgccccatc gtcgtgcgcc ggatgttcaa aggattgttc cagccgcaag
     8101 tagtttgaac cggggcggcc tggtccgcgg gaggcattat gtggccttat gtaatcaggg
     8161 aaccgattga gatcgggacg tacggagtga tgatggcggt cgggttcctg accgcttcgt
     8221 ggtggctggg gcgtgacctt cgccgccgca acatcgaccc ggcggtcggc gacatgacaa
     8281 tcatgattgc gatcgtcctt ggcgtcatcg gctcgaagct ggcttacctt cttaccgaag
     8341 ccgattcgtt cacgctgaag gatttcgttt ccggggccgg attgacctgg cacggcgggt
     8401 tgatcctggc tgccgcgggc atcatcggtt actggctttc gaagaaattt ccgctgccgg
     8461 tcatgcttga cacggtctca ccgatgcttg cttccgggta tgcgttcggt cggctcggct
     8521 gcctgatttc gggtgacggg tgttacggcg tgacatgtca gccttcgtcg tgggatcact
     8581 tcatgtgcat ggcgttcccg aacggaatcg taccgacggc gccgggcgtc gttgttcatc
     8641 ccaccccgct ttat
//
LOCUS       DAOPPA010000075         8610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9171_length_8610_cov_4.227820, whole genome shotgun sequence.
ACCESSION   DAOPPA010000075 DAOPPA010000000
VERSION     DAOPPA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9171_length_8610_cov_4.227820"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1613)
                     /locus_tag="PKH54_03630"
     CDS             complement(405..1613)
                     /locus_tag="PKH54_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99009.1"
                     /translation="MYNRKIYDTQLSKLKGLPLVKVLTGIRRVGKSTILRMFRDRLLQ
                     DGTPPDDILYLDMERMEFANILDAAALHQLAASRLANNGRPRFLFIDEVQQIAGWERA
                     VNSLLNEGGIDIYLTGSNASLLSSELASLLTGRYVEIPVYPLSFAEYATFRGVPADTA
                     LFQEFLIRGGMPGLHMMTNDEEVIRQYLGSLIDSVVLKDVVGRYSVRDVDLLQRLTAF
                     LADNSGNVFSAKRIADFMKNERRSVGVETVYNYISYLISAFLIHRLPRFDLKGKRFLE
                     VSEKYYFADMGLKNALIGYRHSDINAALELVVFLELKRRGYTVSVGRLGDAEIDFIAE
                     RSGERMYIQVCYLLADETVSAREFGPLEALRDNYPKFVLSMDQIGGRDHNGIQRLYLP
                     DFLLAEAIPA"
     gene            complement(1764..3515)
                     /locus_tag="PKH54_03635"
     CDS             complement(1764..3515)
                     /locus_tag="PKH54_03635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC99010.1"
                     /translation="MTKTVREWTLHQEIGRGGMGVVFKATHRFLPDVWAVKVMRPELC
                     EDEESRQRFLSEVMVLSRLRHENIIEIQAPFEEDGHLYLPMEYLTGNSLEQMLKADGS
                     RWPHLRAIDVIAQAARGLGHAHRQVPPVLHRDIKPSNIQVMEDGRVKILDFGLARTIG
                     EKSITATGKAVGTPAYMAPESLSGRKATPQSDVFSLGIVLYRLLCGRLPYDMPEEDSS
                     IHAVFAAVFRGIDKGIPDVREFALDIPPRLARLTMRALSVVPESRPLDGAEFASLLIE
                     SCGGVTADESITTTPPSIETKPGSDATGRPRVISQGKDATRLGIDIENLDVRTSRPVP
                     VQREHMDADHSLLNLPGRLDLLSSSDRGRGKSIEKATHEDRIDRKPIADLADTVFDEN
                     RFRPGKKARVAVLIVVAAVLSVASIWMYFALKPVIATPEQCKEACDRKLGLVAAEQNG
                     NGSVDPVDKVNEAFDKEVQKQEMILGNAIAAIQTECDEAFEALVDKKEIEAAAAICDV
                     HKAAKAEEMAPIFEELRVNREKAVNAAMKLRDEKAAHRDAELAVCTDECIISRTKEST
                     ALCRIAATSLDEFNACI"
     gene            complement(3763..5466)
                     /locus_tag="PKH54_03640"
     CDS             complement(3763..5466)
                     /locus_tag="PKH54_03640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC99011.1"
                     /translation="MVIERALFPDMTRDFGDFQKYCRLLDQGRGGPDGRPRFTRIYGG
                     AEFCVNRLPSAAMTAAVAAMCGDSGLKFTLVLPPVHDTSFSKVDRIVAMMRPGTEIVI
                     NDWAMLEPALAAGCVPVMGRALNRLVRDPRIGSDGRTPQQIEFLQTSQAHLPMVREFI
                     KGRGIARLELDNVRQGLALMPDDGLPASLYTPWTLISVSRFCGSAYAAHCPGKPCFAT
                     RTFVEGVPIIADGTCTFWFNAGMPREMAGIDRLVHVTGPEGLGWLVDELSHPAWDEVE
                     MPRLQSGGEVAVNDPVERIVMQALTDEFKSVCHIGCGFGAHLEVLDRMAKMLTGVDAS
                     MTALDIVRRRVPSAKRICGTAADIAGGPFDLVLDPGFLNDSPDRPAAARRVVESLADG
                     GLYLTSAADEDLDELKILYVDSLAFVWQHKSAVTGRNLVLLRHARPDHDAECRLAGLV
                     EAFLNKGFDSHDIDFVDLTAFAGRTPAAPSLTTWISVTAGHKLADLGFKYLWRELEGE
                     GRTMFRLAHADYRIELEFSPVSQNRPSYRNCGAHNVAYHGNPPDMSTLDAAIDILAPL
                     A"
     gene            complement(5460..6449)
                     /locus_tag="PKH54_03645"
     CDS             complement(5460..6449)
                     /locus_tag="PKH54_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOC99012.1"
                     /translation="MFAINAPLRSHEDVERLVLAGADSFYCGLNDAAGTNGGGGLNQR
                     QSDRSSFSGIADLQRAVTLAHELGREVFIAINTRLLTDTYVDRFTRQLDIAADLGLDA
                     VIVGSMNGLMIARERHPEMKLAGSVGLGVLNSRSARFWADLGVYRVIVSRHLTPREIA
                     MVCDGAPDVEVEAFLMNGKCGNFDSMCGYDYYDPAGAMANFTGCVFVADRNVTRYRRY
                     ACGACALSDFGTRPNFAAAKIIGRDFDVESVTKATELTFKVRAALFSGMSAPEFREFS
                     MAAYRDIYGRPCERNCHYPDEGFPGGGDAFTASAGDEGCSSCEGCDGCGGGTW"
     gene            complement(6541..7092)
                     /locus_tag="PKH54_03650"
     CDS             complement(6541..7092)
                     /locus_tag="PKH54_03650"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013508166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOC99013.1"
                     /translation="MHEDRRVDDPIEQLVRDNIRCIPDFPKPGILFRDITTVLEQPEA
                     FQAALGFFAEAAEKHGATKIAGIESRGFVFAAPVAADLGLPLVLFRKPGKLPFKTRSV
                     KYTLEYGTDTIEVHENSIQPGDRVMIIDDLLATGGTMSAAVRLVREMGAEVAVAAFMV
                     ELPSELNGRARLADVEIECMTAF"
     gene            complement(7112..7876)
                     /gene="surE"
                     /locus_tag="PKH54_03655"
     CDS             complement(7112..7876)
                     /gene="surE"
                     /locus_tag="PKH54_03655"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOC99014.1"
                     /translation="MSKPLILVTNDDGILSTGLDPLIRAAEAHGEVWVVAPDSEKSGV
                     SHAITLTEPLRYTQVGQRRFALNGTPVDCVFIGLNHILPRRPDFVFSGVNRGPNLGFD
                     VLYSGTVGAAMEGTIQKVPSAAFSMVSKGSFDFSEIGAAVDSMVARIVLNGVPDGVTL
                     NVNIPDRTIGEFKGFKVTRLGNRQYSNEVLTREDPRGGKYLWVGGTRVTNELSPDSDT
                     GAVAEGWGSISPVSPDLMAHHAVKHLAWANVPDDQE"
     gene            complement(7914..>8610)
                     /locus_tag="PKH54_03660"
     CDS             complement(7914..>8610)
                     /locus_tag="PKH54_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC99015.1"
                     /translation="VKAGGQEDFFDVPVANMNLNGLTSSITLTAAFDNRDDRMFPTKG
                     NVTSLSMEWAGSEIGSSFDYLRFRASTKQYFPLFLGAVGKIALRYGYIFNPKGGPVPI
                     FQRFQCGGIFTIRGYEQASLGPSIPVGASRDPASSLSYFTIGGNKELIVNFEVEFPIL
                     KAMGIKGVVFFDAGNAFDDTEWPNPLNFQLAAGLGIRWWSPIGPLRFEWGFPINPREG
                     DPPVVFEFNIGAF"
BASE COUNT         1699 a         2567 c         2577 g         1767 t
ORIGIN      
        1 ccgtgatatt cgctcaattt gactgttcga ctggatgcct ttgcttccct cttcatattt
       61 caagaacagc catgatcgcg cggtgatccg acgggaactt tggtctcttg caccgatgtg
      121 ggccgcgtcc atcccggcca tcacgctcca gtcgggcggc gaccttgtga tcgtaaacgc
      181 ccagaaaacc ccgcttgatc cgtgggcgac gctccgctat tccgatctcc gcgaattcgc
      241 cgccgccgtc cttgacgttt ggggattccc cggtcaattt ggattaaaat cagggatttg
      301 ttgttgacaa tcccaaacat gattcgccgc cacccggtcg tgtttgccac atttccgtac
      361 aggccgtgtg atgcgtcggc accaacgacg ggcaatccgt tcgttcaggc aggaatggct
      421 tcggccagca ggaagtcggg aaggtaaagg cgctggatgc cgttgtggtc ccggccgcca
      481 atctgatcca ttgacagcac gaattttgga tagttgtccc gcaaagcctc aagcgggccg
      541 aattcgcgcg cggacacggt ttcgtccgcc agcagatagc aaacctggat gtacatccgt
      601 tcgcccgagc gctcggcgat gaagtcgatt tcggcatcgc ccaggcgccc gacgctgacg
      661 gtgtacccgc gccgcttcag ttcaaggaag acgaccagct ccagtgccgc attgatgtcg
      721 gaatgcctgt acccgataag ggcgttcttc agtcccatgt ccgcgaagta gtacttttca
      781 ctgacttcaa ggaagcgttt gcccttcagg tcgaagcgtg gcaggcgatg gatcaggaat
      841 gccgaaatca ggtatgagat gtagttgtaa acggtttcga cgccgacgct gcgtcgttcg
      901 tttttcatga agtcggcgat gcgcttggcc gagaatacgt tgcccgagtt gtcggcaaga
      961 aaggctgtca gtcgttgaag cagatcgacg tccctgaccg agtaccggcc gaccacgtcc
     1021 ttaaggacca cgctgtcgat caaggagcca aggtactgcc ttattacttc ttcgtcattc
     1081 gtcatcatgt gaaggccggg catgccgccc cggatcagga actcctggaa cagggccgtg
     1141 tcggcaggta cgcccctgaa ggtggcgtat tctgcaaaag acaggggata gacgggaatt
     1201 tcaacatagc gcccggtaag cagcgaggcg agttcggacg acagcagact cgcgttggat
     1261 ccggtcaggt aaatgtcgat gccgccttca ttcagaagtg aattgactgc acgctcccac
     1321 cctgcaattt gctggacttc gtcaatgaac agaaaccgtg gccttccgtt gttggccagg
     1381 cgggatgccg caagctgatg cagtgcagcc gcatcgagga tgtttgcgaa ttccatccgt
     1441 tccatgtcca gataaagaat atcgtccggc ggcgttccgt cctgaagcag acggtcacgg
     1501 aacattcgaa ggatcgtgct ctttccaacc cttcgaatgc cggtcaggac tttaaccagt
     1561 ggcagcccct tcagtttgga aagttgggtg tcgtagattt ttcggttata catgaaacat
     1621 tctccgaaac tggcaaaagt ttacgatata acccgaattt tgcaagcgga aggccgcgcc
     1681 agggcgtcag gccagcgggg caaggatgtc gatggcggcg tccagggttg acatgtcggg
     1741 ccggggtaaa ggttacggca gaatcatatg cacgcgttga actcgtcgag ggatgtggct
     1801 gcaatcctgc agagcgctgt ggattcttta gtcctggaaa ttatgcactc atcggtgcat
     1861 acggcgagtt ccgcgtctct gtgcgctgcc ttttcatctc gcagcttcat ggcggcgttg
     1921 acggcctttt ccctgttaac cctgagttcc tcaaaaatcg gggccatctc ctctgccttt
     1981 gctgccttgt gtacgtcgca tattgcggca gcagcctcaa tttccttttt atctactaat
     2041 gcttcgaacg cctcatcaca ttcagtctgg attgccgcaa ttgcgtttcc cagaatcatc
     2101 tcctgtttct gaacttcctt gtcgaacgcc tcgttcacct tgtcaaccgg atcaacagag
     2161 ccattgccgt tctgttccgc tgcaacaagc ccaagcttcc tgtcgcaggc ttccttgcat
     2221 tgttccggcg tcgcaatgac tggcttcagc gcgaagtaca tccagatgga cgccacggac
     2281 aggactgctg caacaactat taacacagca acacgagctt tctttcccgg gcgaaaccgg
     2341 ttttcatcaa agacggtatc cgcaaggtca gcaatcggtt ttcggtcaat tctgtcctcg
     2401 tgggttgcct tttcgatcga tttgccacgg ccacgatctg aagacgacag gagatccaga
     2461 cgaccgggca ggttcaaaag ggaatgatcg gcatccatgt gttcccgttg gaccgggact
     2521 ggccttgaag tccttacgtc aaggttttca atgtcgattc caagccttgt ggcgtccttg
     2581 ccctggctga tgacgcgagg gcgcccggtt gcgtccgacc ctggtttcgt ttcgattgat
     2641 ggcggcgtgg ttgttattga ttcatccgcg gttaccccgc cacaggattc tataagcagg
     2701 ctggcgaact ctgcaccgtc cagggggcgg gattccggca cgacggaaag tgcccgcatg
     2761 gtcagcctgg ccagccttgg tggtatgtcc agggcgaatt cgcgtacatc gggaatgccc
     2821 ttgtcaatac cgcggaaaac tgcggcgaat accgcgtgga tggaagaatc ctcctccggc
     2881 atgtcatacg gaagacgtcc gcacagcaat cggtaaagga cgatcccaag gctgaagaca
     2941 tcggactgcg gggtggcctt gcgtccactc agagactccg gggccatgta agccggagtg
     3001 ccgaccgcct tgccggttgc cgtgatggac ttttcgccaa tggttctggc caggccgaag
     3061 tccagtattt tcacgcgtcc atcttccatg acctggatat tgcttggttt gatatcccga
     3121 tgaagcaccg gcggcacctg cctgtgcgca tggccaagtc cccgggccgc ctgtgcaata
     3181 acgtcaatgg cgcgaagatg cggccacctg gaaccatccg ctttcagcat ctgctccagc
     3241 gagtttccgg tcagatactc catgggaagg tagagatggc catcctcttc gaagggggcc
     3301 tgaatctcaa tgatgttttc atgtctaagt cgagacagaa ccatcacttc ggacaggaat
     3361 cgctggcggg attcttcatc ctcgcaaagc tcgggacgca tgaccttgac ggcccagaca
     3421 tcaggaagaa accggtgagt cgccttgaaa acgacgccca tgccgccccg cccgatttcc
     3481 tgatgaagtg tccattcacg aacagttttt gtcatttgcc cggattcccg ccaatacacg
     3541 aggatgcgac tcggactcca tcatcaagga cgattcccgg aatcatgagt cggcgcgtcg
     3601 attctatact ctgcagggtg gatgtaaaac cgaataatcg attacggact gcgcttcgtg
     3661 agtgcagaag cgtgtccgcc agggtactgc ttctgaatct cggaacaatc ccgttttcaa
     3721 agaagaacct tttaagccgg gtccgttgaa tgaagcattc ggtcaggcca gcggggcaag
     3781 gatgtcgatg gcagcgtcca gggtggacat gtcgggcgga ttgccgtgat aggcgacgtt
     3841 gtgggcgccg cagttgcggt acgacgggcg gttctgggac accggcgaaa attcaagttc
     3901 gatgcggtag tcggcgtggg ccaggcggaa catcgtgcgg ccttcgccct cgagttcgcg
     3961 ccaaaggtac ttgaacccca gatcagccag cttgtgccct gccgtgactg aaatccaggt
     4021 ggtcaacgat ggcgccgccg gagttcggcc cgcgaaagcg gtcagatcca cgaagtcgat
     4081 gtcgtgactg tcaaaacctt tgttaaggaa ggcctcgaca aggccggcca ggcggcattc
     4141 ggcgtcgtgg tcgggccgtg cgtggcgcag cagaaccagg ttgcggccag tgacggccga
     4201 cttgtgctgc catacgaagg ccagcgaatc gacatacagg atcttcagtt cgtccaggtc
     4261 ttcgtctgcc gccgaagtaa ggtacagccc gccatcggcc agggattcga cgacccggcg
     4321 cgcggccgcc ggcctgtcgg ggctgtcgtt gaggaaaccc ggatccagca ccaggtcgaa
     4381 cgggccgccg gcgatgtcgg ccgccgttcc gcaaatgcgc ttcgccgaag ggacgcggcg
     4441 cctgacaatg tccagcgcgg tcatcgaagc atcgaccccg gtcagcattt tcgccatgcg
     4501 gtcaaggact tccaggtgcg cgccaaagcc gcagccgatg tggcacaccg acttgaactc
     4561 gtcggtcagg gcctgcatga cgatacgctc gaccgggtcg tttaccgcga cctcgccacc
     4621 cgactgcagg cgcggcatct cgacttcgtc ccaggcgggg tgcgaaagct cgtccacaag
     4681 ccagcccagg ccttcaggcc cggtgacgtg tacaaggcgg tcgatcccgg ccatttcgcg
     4741 cggcatgccc gcgttgaacc agaacgtgca tgtaccgtcg gcgatgatcg gcacgccctc
     4801 gacaaacgtc ctggtcgcga agcagggctt gccggggcag tgggccgcat aggcggatcc
     4861 gcagaacctg gaaaccgata tcagcgtcca cggggtgtac aggctggctg gcagtccgtc
     4921 gtcgggcatg agcgccaggc cctggcggac gttgtcgagt tcgaggcggg cgatgccgcg
     4981 gcccttgatg aattcgcgga ccatcggaag gtgcgcctgc gacgtctgaa ggaattcaat
     5041 ctgttgcggt gtgcgtccgt ccgatccgat ccttggatcg cgcacgagcc ggttcagggc
     5101 gcgacccatc accggcacgc atccggccgc aagggccggt tccaacatcg cccagtcgtt
     5161 gatgactatc tcggtgccgg gccgcatcat cgcgacaatc cgatccacct tcgaaaacga
     5221 cgtgtcatgg acgggtggca ggacaagggt gaacttcagg ccgctgtcgc cgcacatggc
     5281 cgcgaccgcc gccgtcatcg ccgctgaagg caggcggttg acgcagaact cggcgccgcc
     5341 gtatatcctt gtgaacctgg gccggccgtc cgggccgccg cgcccctgat cgagcaggcg
     5401 gcagtacttc tggaagtcgc cgaagtcacg ggtcatgtcg gggaacaggg cgcgttcgat
     5461 taccatgttc cacccccgca gccgtcgcag ccttcgcaac tgctgcagcc ctcgtcgccg
     5521 gccgacgccg tgaatgcgtc gccgcccccc gggaatcctt cgtcgggata gtggcagttc
     5581 cgttcgcatg gccgaccgta tatgtcgcga taggccgcca tcgagaattc cctgaattcg
     5641 ggcgcggaca tgccagaaaa gagcgcagcc ctgaccttga aagtcagttc ggtcgccttt
     5701 gtcaccgatt ccacgtcgaa gtcgcgtccg atgatcttgg ccgcggcgaa gttcggcctg
     5761 gtcccgaagt ccgaaagggc gcaggcgccg cacgcgtatc gccggtacct ggtgacgttc
     5821 cggtcggcga cgaacacgca cccggtgaaa ttggccatcg cgccggccgg gtcgtaatag
     5881 tcgtacccgc acatcgagtc gaaattgccg catttgccgt tcatcaggaa tgcctcgacc
     5941 tcgacgtcgg gcgcgccgtc gcagaccatc gcaatttcgc gcggcgtcag gtgccgcgac
     6001 acgataaccc ggtaaacgcc caggtcggcc cagaacctgg ccgaccgcga attcaggacg
     6061 ccaagtccga cgctgccggc aagcttcatt tccgggtgcc tttcgcgcgc gatcatcagg
     6121 ccgttcatgg atccgacgat taccgcatcc aggccaaggt cggcggcgat gtcgagctgg
     6181 cgcgtgaacc ggtcaacata ggtgtccgta agcaggcgcg tgttgatagc gatgaagacc
     6241 tcgcggccaa gttcgtgggc cagggttacg gcccgctgca ggtcggcgat gcctgaaaag
     6301 ctggaacggt cgctctgccg ctggttcagg ccccctccgc cgtttgtccc ggccgcgtcg
     6361 ttcagcccgc agtaaaagga atccgccccg gccaaaacca gccgttcgac gtcctcgtgg
     6421 ctgcgcagcg gggcgttgat tgcgaacatc gggaagctac ccgcggataa agaccttgcg
     6481 attgtatccg aatcgggcgc accggtgtta cgtcaagggg gttaaggtca gcggtttcgg
     6541 tcagaaggcg gtcatgcact cgatttcgac gtcggccagc ctggcgcgtc cgttcagttc
     6601 ggacggaagc tcgaccatga acgccgcgac cgccacctcg gcgcccatct cgcggaccag
     6661 ccgcaccgcg gcagacatgg tgccgccggt cgcaagcagg tcgtcgataa tcatgacgcg
     6721 gtcgccgggc tgaatcgagt tctcgtgaac ctcgatggtg tccgttccgt attcaagtgt
     6781 gtacttcacg ctgcgggttt tgaagggaag cttgcccggc ttgcggaaca gcaccagcgg
     6841 caggccaagg tcggccgcga ccggcgccgc gaacacgaag ccgcgcgact cgatgccggc
     6901 gatcttggtc gcgccgtgct tttcggccgc ttcggcaaag aatcccagcg cggcctggaa
     6961 cgcctcgggc tgttccagga cggtcgtgat gtcgcggaaa aggatgcccg gcttcgggaa
     7021 gtcgggaatg cagcggatgt tgtcgcggac aagctgctca atcgggtcat cgacgcgtct
     7081 gtcttcgtgc atcttcagcc tccctgaaaa gtcattcctg atcgtccggg acatttgccc
     7141 aggccaggtg cttgactgcg tgatgggcca tcaaatccgg gctgaccggg ctgatcgacc
     7201 cccagccttc tgcgaccgcg ccggtgtccg aatccgggga aagctcgttc gtgacgcgcg
     7261 tcccacccac ccacaggtac ttgccgcccc gcggatcctc gcgcgtaaga acctcgtttg
     7321 aatactggcg gttgccgagc ctggtgacct tgaacccctt gaattcgcca atcgtccgat
     7381 ccggtatgtt gacgttcagc gtgaccccgt ccgggacacc attcaagaca atcctggcaa
     7441 ccattgaatc cacggcggcc ccgatctcac tgaagtcgaa tgaacccttt gaaaccattg
     7501 agaatgccgc cgatggaacc ttctgtatgg ttccttccat ggcggccccg accgtgcccg
     7561 aataaagcac gtcgaaacca agattgggcc cgcgattcac gccggaaaag acgaagtccg
     7621 gccgtcgcgg cagaatgtgg ttcaagccga tgaacacgca gtcaaccggt gttccgttaa
     7681 gcgcgaagcg cctttggccg acctgggtgt atcgcagcgg ctcggtcagg gtgatcgcat
     7741 gggaaacgcc gctcttctcg gagtccggcg ccacgaccca gacctctccg tgggcttcgg
     7801 cggccctgat aagcggatca agtccggtgg aaaggattcc atcatcgttc gtgacaagga
     7861 taagtggctt gctcataact ggtcgattcc gtattaagtg tcgcccgcat acgtcagaag
     7921 gcgccgatat tgaactcgaa cacgaccggc gggtctccct cgcgcggatt gatcgggaaa
     7981 ccccactcga aacgcagcgg tccgatcggt gaccaccacc ggatgccaag gccggccgca
     8041 agctggaagt tcagcggatt cggccattcc gtatcgtcga aagcgttgcc ggcgtcgaag
     8101 aacacgacac ccttgattcc catcgccttc aggatcggga attcgacttc gaagttgacg
     8161 atcagttcct tgttgccgcc aatcgtgaag tacgacagcg aggatgccgg gtcgcggctg
     8221 gccccgacgg ggatcgatgg gcccagggat gcctgctcgt atccgcggat cgtgaagatg
     8281 ccgccgcact ggaagcgctg gaagatcgga accggtccgc ccttcgggtt gaaaatgtaa
     8341 ccgtaccgaa gcgcgatctt tccaacggcg cccaggaaaa gcgggaagta ctgcttggta
     8401 cttgcgcgga accgaaggta atcaaagctg gatccgatct ccgaacctgc ccattccatg
     8461 ctcaggctgg tgacgttgcc cttcgtcggg aacatgcggt cgtcgcggtt gtcgaacgcg
     8521 gccgtcagcg tgattgaact ggtcaatccg ttcaggttca tgttggcgac gggaacgtcg
     8581 aagaagtcct cctgtccgcc ggccttgaca
//
LOCUS       DAOPPA010000076         8597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9187_length_8597_cov_3.427535, whole genome shotgun sequence.
ACCESSION   DAOPPA010000076 DAOPPA010000000
VERSION     DAOPPA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_8597_cov_3.427535"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1925
                     /locus_tag="PKH54_03665"
     CDS             327..1925
                     /locus_tag="PKH54_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC99016.1"
                     /translation="MACKEIDRERQVLVSMNPATFETLGEVHLCSADEVRGAVDSAQA
                     AFPGWAALTVRERAGYLLKARDYMLEHADEIAMLISRENGKPLSEALNAEVLVASELI
                     TRYAARAPELLRDRPIELGNPMMNFLKESRLVYRPLGVISVVSPWNYPFSIPIGGLVF
                     SLITGNTVVFKPASDVAMVGMKIDEILNVGGQLPKGVLNTVIASGRTIGETLFSPPVR
                     RIVFTGSTEVGRTIQGIAAKNFIPTSMELGGKDPMVVLEDADLDLATSGAVWGAFTNC
                     GQVCASVERVYVAKKIYDRFVEMVVRKTRALRVGVGQSYDIDIGAMANEEQYNQVLDH
                     IREAVEMGAKIECGGKRPEGAGPGYFIEPTVLTNVTHDMKAVRDETFGPLMPIIPFDT
                     VDEAVRMANDSVYGLCASVWTKDRRLGREVAKRIESGTVAVNDAVFTFALVETPWQGM
                     KESGVGRSHSDEGLLEFVFPQHINEDRSPGFMRRRMWWFPYSEHSYNLLKSAMHAFAN
                     MAKFPSLGWQVMTKKSYRNMFLKD"
     gene            1959..4151
                     /locus_tag="PKH54_03670"
     CDS             1959..4151
                     /locus_tag="PKH54_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOC99017.1"
                     /translation="MNERAMNELQEIRSLTSGYMPGADLQRVEDVLELARVESGGDPS
                     VLAAAMRIVRPVCRLKLDVPSICAAIALAMPPGPDGGPSTRGLDEDSARLAEGVIGLG
                     RFQLLSSKSGQAEAFKRMLVALAGDIRVLLVKMAMRLAELHQTSDDPGAEQIRVAEEA
                     MMIYGPLAERMGISWLRTEIEDEAFRVMRPTEYASLKLAADRRLAERQEFIAGVVGRL
                     QAMIVQGGLAGFEVFGRQKNLYGIWKKMQIQGIELEQVYDFVAFRVIVQKPGDCWQVL
                     GLVHDTWTPIPARFKDFINVPKPNGYRSLHTSVFGPLGEPMEIQIRTWEMHREAESGI
                     AAHWSYKESGRIEVRDSERLGWMKQVVEWAREIQSGRETEQGPLIEEAREGLLVDQVF
                     VFTPQGDLRVLRQGATCLDFAYDVHTEVGHSCTGVRVNGRLVPLSTELVSGDVVEVMT
                     SRTARPRRDWLNFVVSSKARAKIRSWFLEQDRREALEVGRQLLEKEFRRAGAHVVKYF
                     KELLLDEEKGRKVAESFRIGSRDELIRAVGHGRVSAADVVKAVVPVAAPGVPEPERPE
                     ILPKKLRSPTGIEVDGIDGMMVHIARCCNPIPGDGIVGYVTRGRGVTIHTVSCRSVLD
                     GDPERVLPATWNSGSGGTFNVPVRITVRDVPGILAGVTTEIGRQGANIAGVSTKRDDS
                     GLQQVDVVLQVADQSMLDGIVRSVRRLRGVVEVMRVRGPGVSAVAERR"
     gene            4244..6976
                     /locus_tag="PKH54_03675"
     CDS             4244..6976
                     /locus_tag="PKH54_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99018.1"
                     /translation="MKRFQYVICLTFAVSGFAGCGGGNQIGDVVGDVPDEDRIVELDV
                     THDEGQPDEGRDIGRDEGEPGDSVDDRFVDDSDVNGDIDDIDGGDESGGDTPETDGCP
                     GETCEPCPDDGLFCTEEYRDSLGECQIRVKPEFCMIGGECLFENQPSPDDMCLACRPA
                     VSGSEYSPAEGMPCDDADPCTAGDTCDADGICQPGAGFGCDDDNECTIDRCDPNFGCT
                     NTPRASTCDDHDSCTYQDQCNVQTGVCKGFPVNCNDGNECTRDYCDSFDGCQHEPISK
                     ACNDFDGCTLNDMCNEDGVCQGTPMDCSDGIECTVDSCMSGVCLNDWHYGPCNDGDMC
                     TDNDFCNGYHECAGIPKTSCDDYNVCTTDYCDPAVGCVNEFNTVECEPDSACEINGVC
                     AGGVCVGQPRNCDKGNPCMIYSCNPDTGCKEIPTTGVCDDHNACTINENCSTGACIPP
                     EGPAGVTNCDDLNDCTVDNCVPAVGCVHTPQEGECQDPNPCSLTGTGFCRNGECISNV
                     RDCDDNQPCTLDTCDGAGNCIHPPISGFCDDGNHCTFDEVCIGGDCIEGKKVDCNDSN
                     VCTLDECNPDYGCVYTPTGGSCSDGDVCTINDGCANGTCVGTVRACEDYNVCTDNGCN
                     KTSGCFFTPNSVECDDFNICTFDDICGAGECHGVSVFEDPATKAATLTYGVNGNLGQG
                     VDVDDDPSTCAPKGSCVQGIDNAFSRLHWLFNTEMLNASSDGSWSLLLEHDGLKVNGT
                     AYNLNLYWGARIDPVTCDPTTSGCNYGVYTSSLSPTCGPLDVFDNAVINGTKLKAGGA
                     TYEVPVSIVFGDTRRELVLYRAKVTANVSLAGGKVVGGAGALGGAFVVQQLVEALESM
                     PESAFLPRYNKEIILAYIDIYLKPDIDLDGDGTDESVSVGMPFSIVTGHLIGPI"
     gene            7008..7610
                     /locus_tag="PKH54_03680"
     CDS             7008..7610
                     /locus_tag="PKH54_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99019.1"
                     /translation="MRVQNGSILAVLPLLAVIGASCGGSSDADLVEAAFLEFQTGLAE
                     AKGDVLWGIVDDSTRTYFEELAKSIRETCRLVSEAYPSDDRAATLRSVGGDILASARD
                     GKSLFVYMLDARRLVPPKDPDSAKVLEIKLNGRDATVVTRSGEVVAFARNADGKWGTR
                     MFLEAFRELPAIATLKDNIRTAHENCRMLGIDVDAYVTGK"
     gene            7641..8555
                     /locus_tag="PKH54_03685"
     CDS             7641..8555
                     /locus_tag="PKH54_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOC99020.1"
                     /translation="MQKPSCLRQGDTVRIIAPASPFSRQKFMAGVTILRELGLKPVWR
                     DDIFSSCDYLAGDDARRAGEMVQAFRDPDSAAVFPARGGFGCARTCIAIGNEVELPPK
                     LLVGFSDITALHAFFGRKGVVTIHGPNVTTLPSMEPDTLEQYRRTIFGLDRGRDQAWD
                     GLRPVTPGRAAGSVLAANLTVLVSLAGTSLMPDLRGRILVLEDLNEKPYRLDRMLFQL
                     SLQPGFGQVAAVVFGDFMLEGDDIPLFERTVAGFAGRWGVPVVTGFPLGHGRANDCIG
                     EGVDAELDAADGRLVARAPVSPRGPVNS"
BASE COUNT         1657 a         2388 c         2804 g         1748 t
ORIGIN      
        1 aacccgcgac gtcaaccttg gcaaggttgc actctaccac tgagttattc ccgcgcagtc
       61 accaaagcga gggataaatt acattccgcg catgcctgct gtcaagagat ttttgcgtgc
      121 cgaacgacta ctggcgtgga gttattgaag gacgcggtgc gtgcttccgg atacgccccc
      181 gcttgcggcg ccgccaacca ggtagatgta tccgaaggca cgggtcagtc cgtaatagcc
      241 cggccttcat tccggcctca agatgtccgt tgacaccatg cgttacatgg aagaatcttt
      301 acacagtctt aatcaggggt gtccccatgg cctgcaaaga gattgacagg gaacgtcagg
      361 tgctggtgtc catgaatccg gctactttcg agactcttgg agaggttcat ctttgttcgg
      421 ccgacgaggt caggggcgcc gtcgattccg cgcaggcggc gtttcccggg tgggcggccc
      481 ttacggtccg tgaaagggcc gggtacctgc tgaaggcacg cgattacatg ctcgagcatg
      541 ccgacgagat agcgatgctg atttcccgcg aaaatggcaa gccgctttcc gaggccctga
      601 atgccgaggt gcttgtcgct tcggaactga tcacccgcta tgcggccagg gcccccgaac
      661 ttctgcggga tcgtcctatt gaactcggca atccaatgat gaattttctg aaggaatcca
      721 ggctggtcta tcgtccgctt ggcgtcattt cggtggtttc gccgtggaac tatccgttct
      781 ccattccgat tggcggcctt gtcttttcgc ttatcacagg taacacggtg gtcttcaaac
      841 cggcctctga cgtcgccatg gtcggaatga agattgacga aatcctcaat gtcggcggtc
      901 agttgccaaa gggtgtcctt aacacggtca tcgcatcagg gcgcaccatc ggcgaaaccc
      961 tgttttcgcc gccagtccgc aggatagtct tcacgggaag tacggaagtc ggccggacaa
     1021 tccagggaat cgccgcgaag aacttcattc cgacctcgat ggaacttggc ggcaaggatc
     1081 cgatggtggt gcttgaagac gccgacctcg accttgcgac gtctggggcg gtctggggcg
     1141 ccttcaccaa ctgcggccag gtatgcgcct cggtcgagcg tgtgtatgtc gcgaaaaaga
     1201 tttacgacag gttcgtcgaa atggtcgtgc ggaagacgcg ggcccttcgg gtcggtgtcg
     1261 gccagtcgta cgacattgat atcggcgcga tggcgaacga ggaacagtac aaccaggtcc
     1321 tggaccacat cagggaagcc gtcgagatgg gggcgaagat tgaatgcggc ggaaagaggc
     1381 cggaaggcgc cgggccgggc tatttcatcg agccgacggt gctgacgaac gtcacgcacg
     1441 acatgaaggc cgtgcgggat gagaccttcg gcccgctgat gccgatcatc ccgttcgata
     1501 ccgtcgatga agcggttcgg atggcgaatg attccgtcta tgggctctgc gcgtcggtct
     1561 ggacgaagga tcgccgtctg gggcgcgagg tggcgaagcg catcgaatcc ggaacggtgg
     1621 cggtcaatga cgccgtcttc acttttgccc tggtcgagac cccgtggcag ggaatgaagg
     1681 agtccggcgt cggccgttcg cattccgacg aggggctgct cgagttcgta tttccgcagc
     1741 acatcaacga ggacaggtct cccggattca tgcgccgcag gatgtggtgg ttcccgtact
     1801 cagagcattc ctacaacctt ctgaagtcgg ccatgcacgc tttcgccaac atggcgaaat
     1861 tcccgtccct gggatggcag gtcatgacaa agaagtccta ccgcaacatg ttcctgaagg
     1921 actagtccgg attttcggta atatccggtg gtcgtctgat gaatgaacgc gcaatgaacg
     1981 aacttcaaga gatccgatcg ttgacgtcgg gatacatgcc gggcgccgat ctgcaaaggg
     2041 tcgaggatgt ccttgaactg gcgcgggtcg aatccggcgg cgatccgtcg gtgcttgctg
     2101 ccgcgatgcg gattgttcga ccggtgtgcc gtttgaagct ggacgtgccg tcgatatgcg
     2161 ccgccatcgc gctggcaatg ccgcccgggc cggacggcgg tccatccacg cgtggcctgg
     2221 acgaggattc ggcgcgcctg gccgagggcg tcatcggtct tggtcgtttc caactccttt
     2281 ccagcaagtc cgggcaggcg gaggcattca agaggatgct ggtcgcgctt gccggcgaca
     2341 tccgcgtgtt gctggtcaag atggccatgc gcctggcgga gcttcaccag acctcggacg
     2401 atccaggggc cgagcagatc cgggtcgccg aggaagcgat gatgatttac ggcccgctgg
     2461 cggaaaggat gggtatttcc tggctgcgga ccgagatcga ggacgaggcg ttccgcgtca
     2521 tgaggccgac cgaatacgct tccctgaagc tggcggcgga tcggcgtctg gccgagcggc
     2581 aggaattcat cgcgggcgtc gtcgggcggc ttcaggcgat gatcgttcag ggcgggctgg
     2641 cggggttcga ggtttttgga cgccagaaga acctttacgg catctggaag aagatgcaga
     2701 tccagggcat cgagcttgaa caggtctatg atttcgtggc cttccgcgtg atcgtgcaga
     2761 agccgggcga ctgctggcag gtgcttgggc tggttcacga tacatggacg cccattccgg
     2821 cgcgcttcaa ggatttcatc aacgttccaa agccgaacgg ctaccgatcg cttcatacgt
     2881 cggtattcgg cccgcttggc gagccgatgg aaatccagat ccgtacatgg gaaatgcatc
     2941 gcgaggcaga gtcgggaatc gccgcgcatt ggtcgtacaa ggagtcgggc cgaatcgagg
     3001 ttcgggattc cgagcgcctg ggctggatga agcaggttgt tgaatgggcc cgcgagattc
     3061 agtcgggccg tgagaccgag caggggccgc tgatcgagga agcgcgggaa ggactgctgg
     3121 tcgatcaggt gttcgtgttc accccgcagg gcgacctcag ggttcttcgg cagggggcca
     3181 cctgcctgga tttcgcgtac gacgttcata ccgaggtcgg tcattcgtgc accggcgtgc
     3241 gggttaacgg ccggctggtg ccgttgtcaa ccgaactggt ttcaggagac gtggtcgagg
     3301 tgatgacgtc caggacggcc aggccccgca gggactggct gaatttcgtg gtttcgtcca
     3361 aggccagggc gaagatacga agctggttcc tggaacagga caggcgcgag gcgctcgagg
     3421 tcggccgcca gctcctggaa aaggagttcc gtcgggccgg cgcccatgta gtcaagtatt
     3481 tcaaggaact gcttcttgac gaagaaaagg gccggaaggt tgccgagtct ttccgcatcg
     3541 gaagccgtga cgaactcatc agggcggtcg gtcacggtcg cgtgtcggcg gccgatgtcg
     3601 taaaggcggt tgttccagtc gccgccccgg gggttcccga accggaaagg cccgaaatcc
     3661 ttccgaagaa gctgcgcagt ccgactggga ttgaggttga cgggattgac ggcatgatgg
     3721 tgcacattgc gcgttgctgc aacccgatcc cgggcgacgg aattgtcgga tacgtcacca
     3781 ggggaagggg ggtgacaatt cacacggttt cctgccggtc ggttctggac ggtgatccgg
     3841 agagggtgct tccggcaacc tggaacagtg gctctggcgg taccttcaac gtgccggtaa
     3901 ggattacggt gcgcgatgtc ccgggcattc tggccggcgt caccaccgag atcggcaggc
     3961 agggcgcgaa catcgccggg gtctcgacga agcgggacga cagtggattg cagcaggttg
     4021 acgtggttct gcaggttgcc gatcagtcca tgctggacgg aatcgtccgt tcggttcgac
     4081 ggctgcgcgg ggtcgtcgag gtcatgcggg ttcgcgggcc cggtgtctcg gcggtcgccg
     4141 aacggcgctg actcccgcat tcctcgatta tcagcaagct tttgggattt atagaagtgg
     4201 tccgtttgtg tacaatacac cggaccggat ttcggggtca taaatgaagc gttttcaata
     4261 tgtcatctgt ctgacctttg cggtgtctgg atttgccggc tgcggtggcg gcaatcagat
     4321 tggcgacgtg gtcggggatg ttccggacga ggataggatt gtcgagttgg acgtcacgca
     4381 tgacgaaggt cagcctgacg aaggcaggga catcggaagg gacgaaggcg agcctggcga
     4441 ttccgtggat gaccgcttcg tggatgattc cgacgtaaac ggcgatatcg acgacatcga
     4501 cggcggtgac gagtccgggg gcgatacccc tgaaacggac gggtgccccg gcgagacgtg
     4561 cgagccgtgc ccggacgacg gcctgttctg caccgaggag tatcgcgatt cgctgggcga
     4621 atgccagatt cgcgtgaagc ccgaattctg catgattggc ggcgaatgcc ttttcgagaa
     4681 ccagccttcc cccgatgaca tgtgcctggc ttgcaggccg gccgtcagcg gttccgagta
     4741 ttcccccgcg gaagggatgc cgtgcgacga cgcggatccg tgcactgccg gcgacacctg
     4801 cgatgcggac ggcatttgcc agccgggcgc cggtttcgga tgcgatgacg acaacgagtg
     4861 caccatcgac cgttgcgacc ccaatttcgg atgcacgaac accccgcggg cttccacgtg
     4921 cgacgaccac gattcgtgca cgtaccagga tcagtgcaat gtccagaccg gcgtctgcaa
     4981 gggtttcccg gtcaactgca acgatggtaa cgaatgcacc cgtgactatt gcgattcgtt
     5041 cgacggttgt cagcacgagc ccatttcgaa ggcttgcaac gacttcgacg ggtgcacctt
     5101 gaatgatatg tgcaatgaag acggcgtctg ccagggcact ccgatggact gttcggacgg
     5161 tattgaatgc accgtcgatt cctgcatgtc cggcgtctgc ctgaacgact ggcattacgg
     5221 cccgtgcaac gacggcgaca tgtgcacgga taacgacttc tgcaatggtt atcacgagtg
     5281 tgccggcatc ccgaagacat catgcgacga ttacaacgtc tgcaccacgg actactgcga
     5341 cccggcggtc ggatgcgtga acgaattcaa tacggtcgag tgcgagcccg attcggcatg
     5401 cgagataaac ggcgtgtgcg ccggcggcgt gtgcgtcggc cagccaagga actgtgacaa
     5461 gggcaatccg tgcatgatct attcgtgcaa tccggacacg ggctgcaagg aaattccgac
     5521 aaccggcgtg tgtgacgacc acaacgcatg caccatcaac gaaaattgtt cgaccggagc
     5581 atgcattccg cctgaagggc cggccggcgt cacgaactgc gacgacttga acgactgcac
     5641 cgttgacaac tgcgttccgg ccgtcggttg cgtgcatacc ccgcaggaag gcgaatgcca
     5701 ggatccgaat ccgtgcagcc tgaccggaac cggtttctgc aggaacggcg agtgcatttc
     5761 gaacgtcagg gactgcgatg acaaccagcc gtgcacgctc gacacgtgcg acggcgcggg
     5821 caactgcatc catccgccga tttcgggatt ctgcgatgac ggcaatcatt gcacgttcga
     5881 cgaggtctgc atcggcggcg actgcatcga gggaaagaag gtagattgca acgactcaaa
     5941 cgtatgcacg ctggacgaat gcaacccgga ttacggatgt gtctatacgc cgaccggcgg
     6001 ttcgtgttcc gacggtgacg tctgcaccat caacgacggg tgcgcaaacg gcacctgcgt
     6061 cgggacggtc agggcatgcg aggactacaa cgtctgcacc gacaacggct gcaacaagac
     6121 ttcgggatgc ttcttcacgc cgaattcggt ggaatgcgac gacttcaaca tctgcacatt
     6181 cgacgacatc tgcggcgccg gtgaatgtca cggcgtgtcg gtgttcgagg atccggcgac
     6241 caaggccgcg actttgactt atggcgtcaa cggcaatctc ggccagggcg tcgatgttga
     6301 tgacgacccg tccacatgcg cgccgaaggg cagttgcgtc cagggtatcg acaacgcgtt
     6361 ttccaggctg cactggctgt tcaacaccga gatgttgaac gcgtcgtcgg acggctcgtg
     6421 gtcgctgctg ctggaacacg acgggttgaa ggtgaacggc actgcgtata acctgaacct
     6481 ttactggggc gccaggattg acccggtgac ctgtgacccg acgacttccg gatgcaacta
     6541 cggcgtctat acgtcgtcgt tgtcgccaac ctgcgggccg cttgacgtgt tcgacaacgc
     6601 ggtgatcaac ggcacgaagc tgaaggcggg gggtgccact tacgaagtcc cggtcagcat
     6661 cgtcttcggc gacaccaggc gcgaactggt gctttaccgg gccaaggtca ctgccaacgt
     6721 gtcgcttgcg ggcggcaagg tcgttggcgg ggccggcgcg cttggcgggg cattcgttgt
     6781 ccagcagttg gtcgaagcgc ttgaatcgat gccggaaagc gcgttcctgc cgcgttacaa
     6841 caaggaaatc attcttgcgt atatcgacat atacctgaag ccggacatcg atcttgacgg
     6901 tgacggaacc gatgagtcgg tttccgtcgg aatgccgttt tcgattgtga caggtcacct
     6961 cattggcccg atttaagttt cggcgcaagc cgtcagtggg ggttgcattg cgagtccaga
     7021 acggatcaat cctggctgtt ctgcctttgc tggcggtcat cggggcgtcg tgcggcggca
     7081 gttcggacgc cgatcttgtc gaggctgcct ttctggagtt ccagaccggg cttgccgagg
     7141 ccaagggcga cgtactctgg ggcatcgtcg atgattccac caggacttac ttcgaggaac
     7201 tggccaagtc gattcgcgaa acctgtcgtc ttgtatccga ggcctaccct tcggatgatc
     7261 gcgcggcaac gctgcgatcc gtcggcgggg acatcctggc gtcggcacgg gatggaaaat
     7321 ccctgtttgt ttatatgctt gacgcgagaa ggctggttcc accgaaggat ccagattccg
     7381 cgaaggtcct ggagatcaag ttgaacggcc gggatgcgac cgttgtgacc agatccggcg
     7441 aggtcgtcgc atttgccagg aacgccgacg gcaagtgggg aaccaggatg ttcctggagg
     7501 ccttccggga actgccggcg attgccacgc ttaaggacaa catacggacg gcccacgaga
     7561 actgccggat gcttggaatc gacgttgacg cttacgtcac cggcaaatga cgtcacgtcg
     7621 cgtcggggcg gtatatccag ttgcaaaagc cttcatgcct gcgacagggc gacaccgtaa
     7681 gaatcatcgc cccggcttcc cccttttcac gccagaagtt catggccggt gtcacgattc
     7741 ttcgcgagct tgggttgaag ccggtctggc gtgacgacat cttttcttcc tgcgattatc
     7801 tggccgggga cgatgcccgt cgcgccggtg aaatggttca ggcctttcgc gaccctgaca
     7861 gcgccgcggt cttccctgcc cggggcgggt tcgggtgtgc caggacgtgc atcgcgatcg
     7921 gaaacgaagt cgaattgccg ccaaaactgc tggtcgggtt ttcggacatc accgcgcttc
     7981 atgcattttt tggtcgcaag ggtgttgtca ccattcatgg cccgaacgtc acgacgctgc
     8041 cttcgatgga accggacacg cttgagcagt atcgcagaac catcttcggc ctggatcgtg
     8101 gaagggatca ggcatgggac gggcttcgcc ccgttacccc gggcagggcc gcgggcagcg
     8161 tgcttgcggc gaatctcacc gttctcgttt ccctggccgg gacgtcgctt atgcctgacc
     8221 ttcgaggcag gatcctggtg ctggaagacc tgaacgaaaa gccgtacagg cttgacagga
     8281 tgctgttcca gctttccctt cagccgggct tcggtcaggt cgccgccgtc gttttcggcg
     8341 atttcatgct ggaaggcgac gatatccccc tgtttgaaag gacggtggca ggatttgcgg
     8401 gcaggtgggg cgtccccgtg gtgaccggct tcccgctggg tcacgggcgg gccaacgact
     8461 gtatcgggga aggtgtcgat gccgagctcg acgccgccga cggccggctg gttgcccggg
     8521 cgccggtcag cccccggggg ccggtgaatt catgaagttc gacgaagtcg cgacgatact
     8581 tcagtcgttc actggtg
//
LOCUS       DAOPPA010000077         8528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9279_length_8528_cov_4.551281, whole genome shotgun sequence.
ACCESSION   DAOPPA010000077 DAOPPA010000000
VERSION     DAOPPA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_8528_cov_4.551281"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1102
                     /locus_tag="PKH54_03690"
     CDS             74..1102
                     /locus_tag="PKH54_03690"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOC99021.1"
                     /translation="MSIKIVVCSDFDAISEQAAGFVSGRISAGRSGEGSGFVMGLATG
                     NSPTGMYKHLAKAANAGVFDPSKVRSFNLDEYVGLPGCNPQERATHPESYSYFMIQEF
                     FGLLARKFASARVPAGTIIDQAILDQNLELCHREWREVGSGKGRAIVISESADGYLGW
                     IRDNVLHDYDRQISAAGGIDLQVIGVGGKGHVAFHEAGIGFEGSRMLLVRLDDNTVAN
                     AVRDGHFASADDCPHFAVSMGAELVFQARTVVLLADGPRKVEPVMSALLGPVTPDVPL
                     SFCQEYSARGGELVCFVDAVVGAGILEQRDMLLERGWQVIDRSSESAIVPVRDLRFSR
                     DPLTGRFC"
     gene            1127..2071
                     /locus_tag="PKH54_03695"
     CDS             1127..2071
                     /locus_tag="PKH54_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99022.1"
                     /translation="MNFHHQHRDGTDFVSASTIEIAIDRIGAELIGMNWKRDDGSVVP
                     IIWRNMDRQPPNDGFWRRHAPILFPIVGGIHENRSVATTGETIHFSGLHGFFRNSVVQ
                     MVGATPIADGFELSYRLDSNDATRDMYPWDFVVTVVYTVRPDGVDQAVTVTNTGTRPM
                     PFQLGWHPGINMPVVSGGRKDCAIRLPAGPVTRMFNDENCFLTGRSEPFDTTRDFEFT
                     EDGLQATYMFDCSMVRPDDRFVLVSDVRGRHSVRVAFPDYPHLGVWSDAGAPFVCIEP
                     WQGMDDSVDAEPFDLKFGMVLLAPGLCDTRRASIRLIG"
     gene            2085..3284
                     /locus_tag="PKH54_03700"
     CDS             2085..3284
                     /locus_tag="PKH54_03700"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOC99023.1"
                     /translation="MKSGNLVIGQSGGPTAVINASLVGVVQEARRHGEIGKVFGMRWG
                     IQGFLQEELVDLGSEPDDVLEAVRRTPSSALGSCRLKLKDEHLPLVLDVIRRHDIRFF
                     FYIGGDDSQNTTHRVVEYCRTQGYELYGIGIPKTVDNDLYGTDHTPGYGSAARYVALS
                     AQQGGVLARDMQKVDPVCVYQAVGRDAGWLAAASALAKKYEEDPPHLIYIPERPFSNE
                     KFFADIERCYSRFGFVSIFVGEGIVYSDGTPVSGTQTRDKFGNPEFGAMGGASAAMVV
                     KSLACEHLDCRGEFQVVESLQMAGMDRVSETDRHEAWMAGVTAVRLAVAGNTGLMVSF
                     ERAPGPSYECRYVAVPLLKVNEGKQTMPDHFIDDANSFVTPAFLDYLRPLVGELPEMG
                     RLSYRGI"
     gene            3336..5579
                     /locus_tag="PKH54_03705"
     CDS             3336..5579
                     /locus_tag="PKH54_03705"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC99024.1"
                     /translation="MSSFEYRDGRFFKDGEPFYFVGAEYQYYRDKRSNWASRIEKLKE
                     GGINVICFYMPWRHHIVHDPVDGTISYDFDGRTLDSRDLRTFIRLCQEKGLHMLAKPG
                     PFVHSELNIGGLPDVASPSFNPEIEPVRVWDGRPLFWEYDNTQLPSPYDPTFDGLAKT
                     WLEQVGAVIEPFCAPKGNIIALQLLDETIYCTSNDAPWHFAYDAPDMQAYHRFLESRY
                     GDISRYNAIHGTEYKAYCFVPAFKLEPAVPVARTRRDLLKVIDWGEFQWRIRRDIYAR
                     YQDYLGIDRLPYLTNYAGITPPIVENVPDAAEGATKDTPPEMVKLYPDWWFSQNRVDQ
                     DLDVYEYGMISWLGVAAYNIQDASSEPGELGRNEVFNRYINTARRRRGINIEENWGFA
                     KLYHPLSEFPMIPFFQTLACVAGGCTGYVVFCGVNHGYWLDDLDRTTQKQFRTFPSHA
                     PIGENGETGPMYDAMKLLNDWFVREGADFLKAEMDMDVCLLIVPEYAAVSAWVPDRRS
                     WALDHSIPRAGYGVIEEATVICNENGVNYELAELPALTVAQMLSKKRVAVFLSFFMDR
                     AEQEKLVEFARSGGTLICSGELPEYDGHMEPCTLLKDFIQSGSSGVHYSTGNIFNDQS
                     AFLGNLRVTGWQPSLKCSPGLRAFVYRNGDDHFVFFFNFDRDGTHKKFIEFNGMKLDL
                     SVGSKTCGIVRVTDRVIRSILVKGVNEFEKVTSKVRVKLEHSRRASGIDEWEFDGDGS
                     RYDLHRH"
     gene            complement(5553..6578)
                     /gene="lepB"
                     /locus_tag="PKH54_03710"
     CDS             complement(5553..6578)
                     /gene="lepB"
                     /locus_tag="PKH54_03710"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC99025.1"
                     /translation="MKKKERVANPVVQARKLLKDATRILKYRGGRLAPAARDECGGWL
                     KALAKALDGTRPDTTDRERLESIRQASERLSAAMKASEKLLSTATIREYVESIGTAVV
                     LALLVRAFVFEAFHIPTGSMIPTVNIADHMFVNKYIYGLRIPFTHKDIVGGRMPSRGE
                     VVVFDFPVPGENYGQAFLKRVIGLPGDRVRLSGNVLEINGRPIEVKPIVHEGDCGDAN
                     LAACLCARQVERLGDVEYVSQHIAGTRPGLPETCRNEPEWPVNNPMSFGSPVDNPDFP
                     YVVVPGGHVLCMGDNRDNSSDGRYWGFVPIDNIRGRATFFWWPPSKMFRKVSQTVPES
                     VPVKVVP"
     gene            6748..8502
                     /locus_tag="PKH54_03715"
     CDS             6748..8502
                     /locus_tag="PKH54_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC99026.1"
                     /translation="MHRVILMEPGQQLYLEVPAEGVERFVQRLDLARFIQAGMEITRV
                     TIAGERIILQLDWDGVGSETVIIRRADDQPAFMSTRRLSLSVAGASAGRAWQVLLSRI
                     AVSSGDADIDDLIFVLAGGVPDNMADVADSPINAWGSGDSWSQFFCDHAMARKFYEAL
                     QFEDNACFLVHGDLECKFITPRFRATLPRFFNYPWKIFGESEGRDPLTDLTDHDVIYG
                     GEQRLSDHIEGMLSNQAVRGLVIINSTCVPVVIGDDVEKVMEKFRDRCTHGMYHISPQ
                     TENPTEIMMQYLDDARERAVREERPDDGSIALVGFRAGRTHGELRNLISEVGIEVKGT
                     ILPRASARLMERIMKADVLVFRPNVFMTELYDRVFLDCGRRRILPAPPFGLAATARWL
                     MEIAASVGMEDRAAQVIESRLAGIAGVMEKLRLQAAQYEMTFVADPSSPARLLDPVSA
                     TGLAPLPAVAELGYRIRVLARDDFPALYRDFCDRIGTWAAASGVEVQVQGFSDSDSLA
                     AAIASGSGGAVFSEFFYDSRISRHGRHQFSARDFEMGFEGAVRTAQRLLRLAANPFYD
                     RYSGFIATVGSSRNWWKS"
BASE COUNT         1646 a         2437 c         2652 g         1793 t
ORIGIN      
        1 ccgtgctttc ggcgcgcctt catgataaat tccggcctgg ttcgccgtgc gcttgcggaa
       61 ctggagaggc aggatgtcca taaaaatagt tgtttgcagt gatttcgatg cgataagtga
      121 acaggctgcc ggctttgttt ccggcaggat ttcggctggc cgatccggcg aagggtccgg
      181 cttcgtgatg ggcctggcga ccggcaacag cccgacaggc atgtacaagc acctggcaaa
      241 ggcggccaac gccggcgttt ttgatccgtc gaaggtaaga agcttcaacc tcgacgaata
      301 cgtcgggctt ccaggctgca acccgcagga acgggcaact catccggaaa gttattcgta
      361 cttcatgatt caggagttct tcgggcttct ggcaaggaag ttcgcttcag ccagggttcc
      421 ggccggaacc ataatcgacc aggccatcct ggaccagaac cttgaactgt gccatcgcga
      481 gtggagggag gtcggttcag gcaagggacg cgccatcgtg atttccgaat cggcggacgg
      541 ataccttgga tggatccgtg acaacgtgct tcacgactac gatcgccaga tatcggcggc
      601 cggcggtata gatcttcagg tcatcggcgt tggaggcaag ggacatgtgg cttttcacga
      661 ggccgggatc ggtttcgagg gaagccggat gcttcttgtc aggcttgacg acaacacggt
      721 cgcgaacgct gtcagggacg gccatttcgc cagcgccgac gactgtccgc atttcgccgt
      781 cagcatgggc gccgaactgg tctttcaggc caggacggtc gttctgctgg ccgacggtcc
      841 caggaaggtg gaaccggtca tgtcggcgct cctcggcccg gtcacgccgg acgtgccgct
      901 ctctttctgc caggaatact cggcgcgtgg cggggagctt gtctgcttcg tcgatgcggt
      961 cgtcggcgcg ggcatcctcg agcagcgcga catgcttctg gagcgtggct ggcaggtcat
     1021 cgacaggtct tcggaatccg ccattgttcc ggtcagggat ctccggtttt cccgggatcc
     1081 tttaactggc cgtttctgtt aaggtcttgc catcgggggc agcgtcttga acttccatca
     1141 tcagcatcgt gacggaacgg atttcgtgtc ggcctcgacg atcgaaatcg cgatagacag
     1201 gattggcgcc gagcttatcg gaatgaactg gaaaagggat gacggaagcg ttgtcccgat
     1261 catctggcgc aacatggaca ggcagccgcc gaacgatgga ttctggcgtc gtcacgcccc
     1321 gatcctgttt ccgatagtcg gtggcattca tgaaaaccgc tcggtcgcga caaccggcga
     1381 aacgattcat ttttccgggc tgcacggctt cttcaggaac agcgtcgtgc agatggtcgg
     1441 cgcgacgccg atcgccgacg gtttcgaatt gagttaccgg ctggattcga atgatgccac
     1501 ccgtgacatg tacccgtggg actttgtcgt cacggtcgtc tatacggttc gtccggacgg
     1561 tgtcgatcag gcggtcacgg tcacgaatac gggtacgcgc ccgatgcctt tccagcttgg
     1621 ctggcatcct ggaatcaaca tgcccgttgt ttccggcggc cggaaggatt gcgccatcag
     1681 gctcccggca gggccggtga caaggatgtt caacgacgaa aactgcttcc ttacgggtcg
     1741 cagcgaaccg ttcgacacca cgcgggattt cgagttcacc gaagatggcc tgcaggcgac
     1801 ctacatgttc gactgctcga tggtccggcc cgatgatcga ttcgtgctgg tttcggacgt
     1861 tcgggggcgg cattcggtcc gtgtcgcctt tccggactat ccgcatctgg gcgtctggtc
     1921 ggatgccggc gcgcccttcg tgtgcatcga gccgtggcag ggcatggacg acagcgttga
     1981 cgcggaaccg ttcgacctca agttcggcat ggttctgctt gccccgggtc tctgtgacac
     2041 caggcgggct tcgataaggc ttattggctg acattggtgg agatatgaag tctggaaacc
     2101 ttgtgatcgg gcagtcaggc ggtccgaccg ccgtaatcaa cgcgtccctt gtcggtgtgg
     2161 tgcaggaagc acggcggcac ggggaaatcg gaaaggtctt cgggatgcgc tggggtatcc
     2221 agggtttcct gcaggaagaa ctcgtcgatc tcggttccga gccggacgat gtgctggagg
     2281 ccgtccgcag aaccccgtcg tcggcgcttg gatcgtgccg cctgaagttg aaggacgagc
     2341 accttccgct ggttctcgac gttatccgca ggcacgacat ccggttcttc ttctatatcg
     2401 gcggcgacga ttcgcagaat acgacccacc gagtggtcga gtactgccgg acccagggtt
     2461 acgagcttta cggcatcggc atcccgaaga cggtcgataa cgacctgtat ggaacggatc
     2521 ataccccggg atacggttcc gcggcccgct acgtggccct ttcggcgcag cagggcggcg
     2581 tgctggcaag ggacatgcag aaggtcgacc ccgtctgcgt ctatcaggct gtcggtcggg
     2641 acgcgggctg gcttgcggcc gcgtccgcgc ttgcgaagaa gtacgaggag gacccgcccc
     2701 acctgattta catacctgag aggccgtttt ccaacgagaa gttcttcgcg gacatcgaac
     2761 gctgctattc caggttcggc ttcgtgtcga ttttcgtcgg cgaaggcatc gtctattcgg
     2821 acggaacccc ggtatccgga acacaaacgc gcgacaagtt cggaaacccc gaattcgggg
     2881 ccatgggcgg ggcctcggcc gcgatggtcg tcaagagtct cgcttgcgaa cacctggact
     2941 gccgcggcga attccaggta gtcgagtccc tccagatggc cgggatggat cgcgtttccg
     3001 aaacggacag gcatgaggcc tggatggccg gggtgaccgc ggtgcgtctt gccgtggccg
     3061 gcaacaccgg cctgatggta tcgttcgaaa gggctccagg cccgtcctac gaatgccgct
     3121 atgtggcggt tcccttgttg aaggtgaacg agggtaagca gaccatgccg gatcacttca
     3181 tcgacgatgc gaacagcttc gtgaccccgg cgttcctgga ttacctgcgt ccgctggtcg
     3241 gggagttgcc tgaaatgggg cgactgtcgt ataggggaat ctgatccgga attcacggca
     3301 gttgcttgaa caaaccggaa tataaggagt tttcaatgtc atcattcgaa taccgcgacg
     3361 gtcgcttttt caaggacggc gagccgttct attttgtcgg agccgagtac cagtactatc
     3421 gcgacaagcg ttccaactgg gcgtcccgaa tcgagaagct taaggaaggc gggattaacg
     3481 tcatctgctt ctacatgccg tggaggcatc atatcgtcca cgatcccgtt gacgggacga
     3541 tttcctacga tttcgacggc cggacgctgg actcacgcga cctccggact ttcatcaggc
     3601 tctgccagga aaaggggctt cacatgctgg cgaagccggg tccgttcgtc cattccgagc
     3661 tgaacatcgg tggattgccg gacgtcgcgt cgccttcatt caatcctgaa atcgagccgg
     3721 ttcgggtctg ggacggccgt ccgcttttct gggagtacga caacacgcag ctcccgtctc
     3781 catacgatcc gacgttcgac ggtctcgcga agacctggct tgagcaggtt ggtgcggtaa
     3841 tcgagccgtt ctgcgcgcca aagggaaaca tcatcgccct gcagcttctt gacgagacga
     3901 tttactgtac ctccaacgac gccccgtggc atttcgcgta cgacgccccg gacatgcagg
     3961 cgtaccaccg cttccttgag tccaggtacg gagacatttc gaggtacaac gccatccacg
     4021 gcaccgagta caaggcatat tgcttcgtgc cggccttcaa gctggaaccg gccgttccgg
     4081 tggcgcgcac caggcgggat ctgctcaagg tcatcgactg gggcgagttc cagtggagaa
     4141 tccggcgcga catctatgcc cgctaccagg attacctggg tatcgacagg ttgccgtacc
     4201 ttaccaatta cgccgggatt accccgccaa tcgtcgagaa tgtcccggac gcggccgagg
     4261 gggcgaccaa ggacacacca ccggaaatgg tgaagctcta tcctgactgg tggttcagcc
     4321 agaaccgtgt tgaccaggat ctggacgtct atgaatacgg aatgatttcg tggcttggcg
     4381 tggccgcgta caacatccag gacgcttcat ccgagccggg cgaactgggc cgcaacgagg
     4441 tcttcaaccg gtacatcaac actgcaaggc gccggcgcgg gatcaacatc gaggaaaact
     4501 ggggattcgc caagctttat cacccgcttt ccgagttccc gatgattccg ttcttccaga
     4561 cactggcatg cgtggccggc ggatgcaccg gctacgtggt cttctgcggg gtcaaccacg
     4621 gctactggct ggatgatctg gatcgcacga cgcaaaagca gttcaggacg ttcccgtcgc
     4681 acgcgccgat tggggaaaat ggcgagaccg gcccgatgta cgacgcgatg aagctgctta
     4741 acgactggtt cgtgcgtgaa ggcgccgatt tcctgaaggc cgaaatggac atggacgtgt
     4801 gcctgctgat cgtgcccgaa tacgcggccg tttctgcctg ggttccggat cgtcgaagct
     4861 gggcgctgga tcattcgatt ccccgcgccg gttacggcgt aatcgaggaa gccacggtca
     4921 tctgcaacga aaacggagtc aattatgagc tcgcggagct gcccgccctg acggttgccc
     4981 agatgctgtc taagaagcgg gtggcggtat tcctttcatt cttcatggat cgggccgagc
     5041 aggaaaagct tgtcgagttc gcgcgctccg gcgggacgct catatgcagc ggcgagcttc
     5101 cggaatatga cggacacatg gaaccgtgca ccctgctgaa ggatttcatc cagtccgggt
     5161 cttcaggcgt acattactcg accggcaaca tattcaacga tcagtcggcc ttcctgggca
     5221 atctgcgggt cacgggctgg cagccgtcgc tgaagtgcag ccctggactg cgtgcattcg
     5281 tctatcgcaa cggtgatgac catttcgtct tcttcttcaa cttcgacagg gacggcaccc
     5341 acaaaaaatt cattgaattc aatggtatga agctcgatct ctcggtcggg tcgaagactt
     5401 gcggaatcgt gagggtgacc gacagggtga tccgttcgat actggtcaag ggtgtcaacg
     5461 agttcgagaa ggtcacgtca aaggtacggg tcaagctcga gcattccagg cgggcttcgg
     5521 ggatcgacga gtgggagttc gacggcgacg gttcaaggta cgaccttcac aggcactgat
     5581 tcagggacgg tctgactgac cttcctgaac atcttcgacg gtggccacca gaagaaggtc
     5641 gcccttcccc tgatgttgtc gatcggcaca aaaccccagt aacggccgtc acttgagtta
     5701 tcgcggttgt cacccatgca gagcacgtgt cccccgggga cgacgacgta cgggaagtcc
     5761 gggttgtcaa ccggtgaccc gaaggacatg gggttgttga caggccattc cggctcgttg
     5821 cggcatgttt cgggaagccc cggccgcgtg ccggcgatgt gctgcgagac gtattcgacg
     5881 tcgccgagcc gttcgacctg tcttgcgcac aggcaggccg caaggttggc atccccgcag
     5941 tcgccttcgt gaacaatcgg cttgacctcg attggcctgc cgttgatttc cagcacgttt
     6001 cccgaaagcc tgacgcgatc ccccggcagg ccgattacac gcttcaggaa tgcctgcccg
     6061 tagttctctc cgggcaccgg gaaatcgaat accacgacct cgccgcgcga cggcatccgg
     6121 ccaccgacga tatccttgtg cgtgaaaggt atccgcagac cataaatgta tttgttgacg
     6181 aacatgtgat ccgcgatgtt gacggtcggt atcatcgagc cggtcgggat gtggaacgcc
     6241 tcgaacacga aagcccgcac cagcagggcc agcacgacgg cggttccgat tgattcgacg
     6301 tattccctta tggtggcggt cgaaagaagc ttttcgctgg ccttcatcgc cgccgaaagt
     6361 ctttccgacg cctggcgaat cgattccagc ctttcccggt cggtcgtatc aggcctggtg
     6421 ccgtcaagcg ccttcgcgag cgccttcagc cagcccccgc attcatccct tgcggcgggg
     6481 gcaagccggc cgcccctgta cttcaggatc ctggtggcgt ccttcagcag tttcctggcc
     6541 tggacaaccg ggttggcaac acgttccttt ttcttcatca cgacaccctc acgacacctt
     6601 ggcggattcg atttcagttg cggcgcatgg cgtcgcgagc cggggcaaag aatagaagcg
     6661 ggcatgctca aagtcaaata ccggggaaac gttgacgcgg cttgcggtta aatggattat
     6721 tgttcaccgc cggaaggccg gccggtcatg catcgggtaa tcttgatgga accgggacag
     6781 cagctctatc ttgaagtgcc ggccgaaggg gtcgaacggt tcgtccagcg cctggacctg
     6841 gcccgtttca tccaggccgg catggagatc accagggtca ccatcgccgg tgagaggatc
     6901 atccttcaac ttgactggga cggggtcggc agcgagacgg tcattatcag gcgggccgac
     6961 gaccagccgg ctttcatgag caccaggcgg ctttccctgt cggtggccgg tgcaagcgcc
     7021 ggaagggcct ggcaggtgct tttaagcagg attgccgttt cttccggcga tgccgacatc
     7081 gacgacctga tattcgtcct ggcgggcggc gtgccggaca acatggccga cgtggccgat
     7141 tcgccgatca acgcatgggg atccggcgac tcctggtccc agttcttctg cgaccacgcg
     7201 atggccagga agttctacga ggcccttcag tttgaagaca acgcgtgttt ccttgttcac
     7261 ggggatctcg agtgcaagtt catcaccccc agattccgcg cgactcttcc acgcttcttc
     7321 aattacccgt ggaagatttt cggcgaatcc gagggccgcg atccgttgac ggatcttacc
     7381 gaccacgatg tcatttatgg cggcgagcag cgtctttcgg atcatatcga gggcatgctt
     7441 tcgaaccagg cggtgcgtgg cctggtgata ataaactcca cgtgcgttcc ggtggtcatc
     7501 ggcgacgatg tcgagaaggt catggagaaa ttccgcgacc ggtgcaccca cgggatgtac
     7561 cacatcagtc cgcagaccga gaatccgacc gaaatcatga tgcagtatct cgatgacgca
     7621 agggaacgcg ccgtcaggga ggaaaggcct gacgacggat cgatcgcgct ggtcgggttc
     7681 cgggcgggcc gcacccacgg tgaattgaga aacttgattt cggaggttgg aatcgaggtc
     7741 aagggaacca tccttccaag ggcgagcgca aggctcatgg agcgaatcat gaaggcggac
     7801 gtgcttgttt tcaggccgaa cgtgttcatg acggagcttt acgacagggt atttctcgat
     7861 tgcggaaggc gtcgaatcct tcccgcgccg cctttcgggc ttgccgcgac agcccgttgg
     7921 ttgatggaaa tcgcggcttc ggtcggaatg gaggacaggg cggcccaggt catcgaatca
     7981 aggctggccg gcattgccgg tgtcatggaa aagctgcggc ttcaggccgc gcagtacgag
     8041 atgacgttcg tggccgaccc ttccagtccg gcgcggctgc tcgatccggt ttcggcaacc
     8101 ggactggcgc cgcttccggc ggtggccgaa ctcggataca ggataagggt gctcgcccgc
     8161 gacgattttc cggcgcttta cagggacttc tgcgatcgaa tcggtacctg ggccgcggcc
     8221 agcggcgtcg aagtccaggt tcaggggttt tccgacagcg attcgcttgc ggcggccatc
     8281 gcatccggca gcggcggcgc ggtattttcc gaattctttt acgattcccg catctcgaga
     8341 catggccggc atcaattttc ggccagggac ttcgagatgg gattcgaggg cgccgtgagg
     8401 acggcccaac gtctcctcag gctggccgca aatccgttct acgacaggta ttccggtttc
     8461 atcgccactg tcggcagctc ccgtaactgg tggaagtcat gaagattgct gaccgtcacg
     8521 aattcagg
//
LOCUS       DAOPPA010000078         8496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9317_length_8496_cov_4.025471, whole genome shotgun sequence.
ACCESSION   DAOPPA010000078 DAOPPA010000000
VERSION     DAOPPA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9317_length_8496_cov_4.025471"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PKH54_03720"
     CDS             <1..839
                     /locus_tag="PKH54_03720"
                     /inference="COORDINATES: protein motif:HMM:NF021350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HOC99027.1"
                     /translation="PEVLCMDEPFSGLDVLTADNLRSELMDLWLEKKIPTKAILAITH
                     SIEEAVMMADKVVIFWHNPGRVAAVLKVGLPHPREKKSPEFKKLIDQIYTILVRQAEV
                     ETKASQRPLALRKFQKLPHARLGALSGLLDLIQERGGVEDIYELEGELNMEADDLLEL
                     TEAASVLGLAVLSEGDIKLTQLGMRFVEGDILSRKDVFREAVAGSVVVINQILQALNT
                     ATDHSMDEEYFLDVLENHFTEDEAYRQLQTAIDWGRYAELFAYDEQSGVLYLEDPPEE
                     EG"
     gene            complement(846..2042)
                     /locus_tag="PKH54_03725"
     CDS             complement(846..2042)
                     /locus_tag="PKH54_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC99028.1"
                     /translation="MIPRRRYNIEAGFFNDALTGRLAPSRMRAFVPRFEAALAASLGG
                     RHSVQATGSGRKALATLLDTLGIPKGSTIMIPAYTLGDIGPFLGRLGYRWITCDVDPG
                     RPVMTAATVLDAWPSGGDVKCVLATHLFGIPADVPAIAKAVEPFHATVIEDCAQSIGS
                     TLGGAWTGTLSRGAIFSFNYLKPVNCFGGGAMVLPGHREMLPLKSPPRVDTAGSFARG
                     LAEDLVSAGPWLRLPTALLAFDQFRPTMMMIDRMIRRPPDTAEEPCSISPLQALHGIH
                     SLASLERRLALRRLIARRLCEAAEIDDGLYREDSPARSNAYFFTIRVADAPAFRRELW
                     MQGIDAGIGAEVADYLESPVCPERPAAMGWFREAVQLPCHEWLTPRQVERIVAAIRQS
                     RDCFRR"
     gene            2165..3682
                     /locus_tag="PKH54_03730"
     CDS             2165..3682
                     /locus_tag="PKH54_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99029.1"
                     /translation="MASFDKEIVFLRLPPFFGKFFTHPQECQVPFPIAYAGACLKKDG
                     FKVSVNDQWAREEAPSRMLRGLLAGDMGMLVMHVDSMNRDVVMDTAWAVRRHCGRSLP
                     MIATGQYADVFPESLVGTAGPFDAAVRGEPELTLVEVARAARSWQEAGCPGDGPDFSG
                     IAGVTWSVADRSRIVRNEDRPLIAGLDDLPLPDFGLFDLDRYAKMSSFVPVRGRVRWG
                     WIMSSRGCPFGCLFCSPTLRKSYGTRYRTHSPEYVSSMVHDLVVNHGCNAIAFEDDVF
                     SLDRNRTIEICHRIRESNPGVFWTAQTHLATLDGEVIQAMAEAGCRGLCVGIESGDEA
                     VRRVLKGQSLSDSDIERNVALIHAHGIQLTAYFMVGCPGETLEQMKKTVEMARRIDPA
                     MIQVAFFTPYPGSPAWDRYMVGGRHEPDMSHYNRFSVNLSAAPRKDVERLHDRFYTSF
                     FLRPKWIARYVTRRLPYVVLQSRGREWKLLFNTLVYLFAPVHSVKMDSMDSIRRL"
     gene            complement(3710..4816)
                     /gene="buk"
                     /locus_tag="PKH54_03735"
     CDS             complement(3710..4816)
                     /gene="buk"
                     /locus_tag="PKH54_03735"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOC99030.1"
                     /translation="MSGTASFCVLAVNPGSTSTKIAVFEGDREVFCTELMHSCEELAE
                     FDGKSITAQFAFRRDAILKALADHSMSMKDIDAVSGRGGLLWPMAHGTYAVNPQMVED
                     LQAGVQGDHASNLGGLIANELVRDSGKPAFIVDPVVVDELPPRVKITGIKELRRRSIS
                     HALNQIASCQRFAAETGTSYESLNLIVAHMGGGISIGAHARGKYIDVNDALDGEGPFS
                     PQRSGSLPTGAWMRLVLSGKYTRDQLKLLNKGRGGLIDLLGTADLREVVRRIEAGDRE
                     AADVFEAMCYQIAKAITALLPAFDGERVDQVILTGGMARSKPLVEKITRYIGAMGSPV
                     TVYPGENEMGALAQGAIRVLTGVEKAREYTGRQA"
     gene            complement(5329..7407)
                     /locus_tag="PKH54_03740"
     CDS             complement(5329..7407)
                     /locus_tag="PKH54_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOC99031.1"
                     /translation="MNGVRLLCAASLLVTLLAGCDGGKKFHMTEEGDSFLILNGLGEL
                     ELSRHGFTMVARRDGVEVMGLSEDDGMLSAAALVVGAGLERSVIAIDGISPIDGTGDT
                     FDFYMLSDGRRVALLQIGPSGTDGFTMTMRPDFDAIEQPVQSFGFTFDLGRMGYVYGQ
                     GILTEQKNYREGHFNKGVQRFPMNRGSFERPLFVTDEGTNIVSPLWFTSRGLGLFMDS
                     YSTLDLSFNSDAVVPEGNGLFRIHLVPLEAERELKMDLIVERTTVDAFRAWVARKYST
                     RPDLPVGARPADDLFTRPIWTSWAAFKWEIDRERILSFAHSIVDNGYDLGYLEIDDKW
                     TPAWGDLRFDPVKFPDAAGMISEIRSLGSKVTAWIPPFVNTDAADFQSGVDARAYVAG
                     GPDDEYPMLVGWWDVLFVPYAGTIDFTRSEGRDWWGSRIDSLIDDYGLDGFKYDAGET
                     CFLPKPVTLGSSVPFNAYPDFYAEWGFEHAAAEMRAGWFGQHIPVLFRMFDKNSEWGL
                     ENGMASVLTQYLALGMIGYPFVMPDMLGGNEYVVKCDDELMIRWAQMNALMPCIQYSI
                     LPWRDEFAPTTNEIVKKFHDLRATFADEFIRLADIAARTQMPIVRPLFFQFPDDAVTY
                     GIEDQFMLGDRYLVAPVLEKGATSRGVYIPAGKWAALDDPDEIHDGPKWVEQYPAPIE
                     RLPAFKLVTN"
     gene            7517..>8496
                     /locus_tag="PKH54_03745"
     CDS             7517..>8496
                     /locus_tag="PKH54_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOC99032.1"
                     /translation="MPPPSLKIVIAGAGAIGGYLVSQFSGQGHQVSIIDVNSDRFDRF
                     PSVPRERMYVGNLVSSSVLARAGAGSADIFIASSNQHEANALSAMKARRMGAARVVAL
                     IEKADYFDDPVGIYRDWFGIDLVLNTRFLVAGEIAKVIRTRGVLAVDAFVEHGFEMVQ
                     FRVQAETEHTGRPLRDLRLPEQTLVVALNRDGVLTIPRGDDSVRAGDEVLVLSSVDKI
                     PFVERIFGRSKVAGHKTVILGGGTVGHALARQISGRASNITVIERDLDRCDFLSRELD
                     RTTVIYGDGSDGDLLNEEGVGSAEVFAAVTGDDEKNIIAATLARALGAER"
BASE COUNT         1678 a         2485 c         2543 g         1790 t
ORIGIN      
        1 aaccggaggt cctttgcatg gacgagcctt tttccgggct tgacgtgctt acggccgaca
       61 atctgcgaag cgaactgatg gatctgtggc ttgaaaagaa gatcccgacc aaggccatcc
      121 tggccattac ccacagcatc gaggaagccg tcatgatggc ggacaaggtc gtgatcttct
      181 ggcataaccc aggtcgcgtc gccgcagtgc tgaaagtcgg gctgcctcat ccccgggaaa
      241 agaaaagccc cgagttcaag aagctcatcg accagatcta cacgatcctg gttcgtcagg
      301 ccgaggtcga aaccaaggca agccagcgac ctcttgccct tcgaaagttc cagaagcttc
      361 cacatgcccg ccttggcgcg ctttccggcc tgctggatct gatccaggaa aggggcggcg
      421 tggaagatat ctacgagctt gaaggcgaac tgaacatgga agccgacgac ctgctcgaac
      481 tgaccgaggc ggcctcggtg ctcgggctgg ccgtgctgtc ggaaggcgac atcaagctga
      541 cccagctcgg gatgaggttc gtcgagggcg acatcctttc aaggaaggac gtcttccgcg
      601 aggcggtcgc cggatcggtc gtggtcatca accagattct ccaggccttg aacaccgcga
      661 cggatcacag catggacgag gagtactttc ttgacgtgct ggaaaaccac ttcaccgagg
      721 acgaggcgta tcgccagctt cagaccgcaa tcgactgggg acgttacgcc gagctgttcg
      781 cgtatgacga gcagtcaggc gtgctctacc tggaagatcc gcccgaggag gaagggtgac
      841 cttcgtcatc gtcgaaagca gtcgcgcgac tggcggatgg ccgccacaat cctttcgacc
      901 tgtcttggtg tcagccattc gtggcagggc agttgaaccg cctccctgaa ccatcccatt
      961 gctgccggcc tttccgggca gacgggactt tccaggtagt ccgcaacttc cgcgccaatt
     1021 ccggcgtcaa ttccctgcat ccagagttcg cgcctgaatg cgggtgcgtc agcaaccctg
     1081 atcgtgaaga agtatgcatt tgaacgcgcc ggcgagtctt cgcggtatag cccgtcatcg
     1141 atttcggccg cttcgcacag cctgcgagcg atcagccttc gcaaggcaag ccgcctttcc
     1201 agggaggcca gcgagtgtat tccgtgaagg gcctgcagtg gagatatgga gcacggctct
     1261 tccgccgtat ccggtggtcg ccggatcatg cggtcaatca tcatcatcgt cggccggaac
     1321 tggtcaaatg caagaagggc ggttggcaat ctcagccacg gccccgcgga aacgagatcc
     1381 tcggccaggc cgcgggcgaa ggagccggcc gtatcgacac gcggcggaga cttcagcggc
     1441 agcatttccc tgtggccggg aagaaccatt gcgccgccgc cgaagcagtt gaccggcttc
     1501 aggtagttga agctgaaaat ggcgcccctg gagagggtgc cagtccaggc gccgcccagc
     1561 gtgctcccga tggattgcgc gcagtcctcg atgaccgtcg cgtgaaacgg ttcgactgcc
     1621 ttcgcgatgg ccggaacgtc ggcgggaatc ccgaaaaggt gcgtggccag cacgcacttg
     1681 acgtcgcccc cggacggcca tgcatccagc acggtcgcgg ccgtcatcac cggccttccg
     1741 ggatccacgt cgcacgtgat ccagcgatac cccagccgtc cgaggaaagg cccgatgtcc
     1801 cccagggtgt acgccgggat catgatggtc gagccctttg ggattcccag ggtgtccagc
     1861 agcgtggcca gggccttgcg tcccgatccg gttgcctgga ccgagtggcg accgccgagg
     1921 cttgcggcca gggcggcttc gaatctgggc acgaaggccc tcatccttga tggggcaagc
     1981 ctgcccgtga gtgcgtcgtt gaagaagccg gcctcgatgt tgtaacgacg ccttggaatc
     2041 atcgcttttt ccccgtgcga catgccaatt tggaaatacc ggccgtcatt ctccaggccg
     2101 gcctgacggc agttggcccg ttgtgttaag atatcacgga atccggttcg ctttcggggt
     2161 tgctgtggcc agtttcgaca aagagattgt tttccttcgg ctcccgcctt tcttcggaaa
     2221 gttcttcacg catccacagg aatgccaggt tccctttccg attgcatatg cgggcgcgtg
     2281 cctgaagaag gacgggttca aggtgagcgt caacgatcag tgggcccgcg aggaggcccc
     2341 gtcgcgcatg cttcgcggac tgcttgccgg cgatatgggc atgcttgtga tgcacgtcga
     2401 ttcgatgaat cgcgacgtgg tcatggatac cgcctgggcg gtaaggcgtc actgcggccg
     2461 gtcgcttccg atgatagcca ccggccagta cgccgacgtg ttccccgaaa gtcttgtcgg
     2521 cacggccggg ccctttgatg cggctgtccg tggcgaaccg gaactgactc tggtcgaggt
     2581 ggcgcgcgcc gccagatcct ggcaggaagc aggatgtccg ggcgatggcc ccgatttttc
     2641 cgggattgcc ggcgtgacct ggtccgttgc cgatcgttcc cggatcgtgc gcaacgaaga
     2701 ccgtccgttg attgcaggtc tggacgattt gccgcttccc gacttcggtc tgttcgatct
     2761 ggatcggtac gccaagatgt cgtcgtttgt tccggtaagg gggcgtgtcc gctggggatg
     2821 gatcatgtcc agccgtggtt gtccattcgg ttgccttttc tgttccccga cactgcgcaa
     2881 gtcttacggc acccgctaca ggacacattc ccccgaatac gtgtccagca tggtgcacga
     2941 tcttgtcgtc aatcacggat gcaacgcaat cgcgttcgaa gacgatgtct tttccctgga
     3001 tcgcaatcgc accatcgaga tatgtcacag gattcgcgaa agcaatcccg gcgtattctg
     3061 gacggcgcag acgcatcttg ccacgctgga cggggaagtg atccaggcga tggccgaagc
     3121 tggttgtcgc gggctgtgcg tcggcatcga gtccggtgat gaagcggtcc gtcgagtgtt
     3181 gaagggccag tcgctgtcgg attcagatat tgaaaggaac gtcgcgctga ttcacgctca
     3241 tggaattcag cttaccgcat acttcatggt aggttgtcca ggcgagacgc tggaacagat
     3301 gaagaagacg gttgaaatgg cccgccggat cgaccctgcc atgatccagg tcgccttttt
     3361 cacgccctat cccggcagtc ccgcttggga caggtacatg gtcgggggtc gtcacgagcc
     3421 cgacatgtcg cactacaacc gtttcagcgt caatctgtcc gccgcgccca ggaaggacgt
     3481 cgaaagactt cacgatcgct tttatacatc gttcttcctt cggccgaagt ggatcgcaag
     3541 gtatgtgacc agaaggcttc cgtatgtcgt gctccagagt cgaggccgcg aatggaagct
     3601 tcttttcaac acgctggtct atctgttcgc gccggttcac agcgtgaaga tggattcgat
     3661 ggattcgatc aggcggcttt gacggagtca cgcaccggcg ggaggatcgt caggcctgtc
     3721 gcccggtgta ttcacgcgcc ttttcaacac cggtaagaac ccgaatggcg ccctgcgcca
     3781 gcgcgcccat ctcattttcg cccggataga cggtcacggg cgaacccatg gcgccgatgt
     3841 acctggtaat cttttcgaca agcggcttgc ttcgcgccat tcctccggtc aggatgacct
     3901 gatcgacgcg ttcgccgtca aatgccggca gcaaggcggt gatggccttt gcaatctggt
     3961 agcacatcgc ttcgaagacg tcggcggcct cccgatcgcc ggcctcaatc cgcctgacca
     4021 cttcacgcag atccgcggtg cccagaaggt cgatcaggcc gccgcggccc ttgttcagaa
     4081 gcttcagttg atccctggtg tactttcccg acagcacaag gcgcatccac gcgccggtgg
     4141 gcaggctgcc tgaacgctgg ggactgaacg ggccttcgcc gtcaagcgcg tcgttcacat
     4201 cgatgtattt tccgcgggca tgcgccccga tgcttattcc gccgcccatg tgcgccacaa
     4261 tcaggttcag gctttcatac gatgtcccgg tctcggcggc gaaccgctgg cacgacgcaa
     4321 tctggttcag cgcgtggctg atcgagcggc ggcgaagttc ctttataccc gtgatcttga
     4381 cccgtggcgg aagttcgtca accacgacag gatcgacgat gaatgccggt tttccggaat
     4441 ccctgacaag ttcgttcgcg atcaggccgc ccaggttcga cgcatggtcg ccctgcacgc
     4501 cagcctggag atcctcgacc atttgtggat tgaccgcgta tgttccatgt gccatcggcc
     4561 acagaaggcc gccgcgcccc gagaccgcat cgatgtcctt catcgacatc gagtggtcgg
     4621 caagcgcctt caggatggcg tcgcgcctga acgcgaactg ggcagttatc gacttgccgt
     4681 caaactcggc cagttcttcg cacgaatgca tcagctcggt gcagaagact tccctgtcgc
     4741 cttcgaatac tgcgattttt gtggaagtcg agcctggatt cactgcaagc acgcaaaacg
     4801 aagcggtacc ggacattttt caatcctcgt gaaggtgcgc gattcgggct gtaaccctcc
     4861 ccggcaggat atcacaagca aagtcgttgg aagcgttgag tgaaaaaaag tttgtcggac
     4921 gctggctcgc gtgaatgaaa tcggtcaatt ccgtcagtcg tacggaatat cggttcggcg
     4981 aatctcagta gtagcagtag tcgtacatgt cgtcccaggc gtctgagcaa ctgtagtagc
     5041 cgcagtagaa ggtctgtcca tcggcgacgt agtagcagtc ggtcgcgtac gaatcgtagt
     5101 cgcagcagat cttgggatcg gagcatgtgc ccaggccttc acacctgtat ttgaaaagtt
     5161 cttttcagca gccggtgatc gacagcgcgg cggtgacgat gaaagcgacg acaagaagct
     5221 tgcgaatcat atttttacct ctgaaaggtt caagagtaag cggattttag cacttgtctg
     5281 cgccgtaata aaccccctta agcaagcccc tggattcgtt gtctaatttc agttggttac
     5341 aagcttgaag gccggaagtc gttcgatcgg tgccgggtac tgctcgaccc atttcggtcc
     5401 atcgtggatt tcgtcggggt catcaagggc cgcccatttg ccagccggta tatacacgcc
     5461 gcgggaagtt gcccctttct ccagaaccgg ggccacaagg taccggtcgc ccagcatgaa
     5521 ctgatcctcg atcccgtagg tcacggcatc gtccggaaac tggaagaaga gcgggcggac
     5581 gattggcatc tgtgtccggg cggcgatgtc ggccaggcgg atgaattcgt cagcgaacgt
     5641 cgcgcgaagg tcatggaact tcttaacgat ttcgttcgtg gtcggtgcga actcgtccct
     5701 ccacggcagg attgaatact ggatgcaagg catcagcgca ttcatctggg cccaccggat
     5761 catcagctcg tcgtcgcact tgaccacgta ttcgttgccg ccaagcatgt ccggcatcac
     5821 gaacggatag ccgatcattc ccagtgccag gtactgagtc agaaccgaag ccatgccgtt
     5881 ttccaggccc cattcactgt tcttgtcgaa catccggaaa agtaccggga tgtgctggcc
     5941 aaaccacccg gcgcgcatct cggccgcggc gtgctcgaat ccccattcgg catagaagtc
     6001 tggataggcg ttgaacggaa ccgagcttcc aagcgttacc ggcttcggca ggaagcaggt
     6061 ttcgccggcg tcgtacttga agccgtccag gccgtagtcg tcaatcaacg agtcgatcct
     6121 ggaaccccac cagtcccttc cttccgacct ggtgaagtcg atggtcccgg cgtaaggcac
     6181 gaacagaacg tcccaccatc cgaccagcat cggatactcg tcatccggcc ctccggccac
     6241 ataggcgcgc gcatcgacac cggactggaa gtcggcggcg tcggtgttga cgaaaggcgg
     6301 aatccaggca gtcaccttcg agccgaggga tctgatttcg gagatcattc ctgccgcatc
     6361 gggaaacttg accggatcga aacgcaggtc gccccaggcc ggcgtccact tgtcatcgat
     6421 ttccaggtaa ccaaggtcgt atccgttatc gacaatggaa tgtgcgaagg aaagaatcct
     6481 ttcccggtcg atctcccatt tgaaggcggc ccacgatgtc cagatcggcc tggtgaaaag
     6541 gtcgtcggcc ggcctggcac caaccggcag atccggtctt gtcgagtact tgcgggccac
     6601 ccatgcgcgg aaggcatcga cggtcgtccg ctcgacgata agatccatct tcagttcgcg
     6661 ttcggcttcc agcggcacca ggtgaatcct gaaaaggccg ttgccttccg gcaccacggc
     6721 atcgctgttg aaggaaagat ccagggtcga gtacgagtcc atgaacagtc caagtccccg
     6781 ggacgtgaac cacagtggtg atacgatgtt ggtgccttcg tcggtgacga acagcggccg
     6841 ttcaaacgag ccgcggttca tcgggaagcg ctgcacgccc ttgttgaaat gtccttcgcg
     6901 gtaattcttc tgctcggtaa ggattccctg tccatagacg tatcccatcc ttccaaggtc
     6961 aaaggtgaat ccaaaggatt gaaccggttg ttcaatcgcg tcgaagtccg gacgcatcgt
     7021 catcgtgaat ccgtccgtgc cggatggccc gatttggagc agcgcgacgc gcctgccgtc
     7081 cgacagcatg taaaaatcga atgtgtcgcc agttccatcg atcggggata tcccgtcgat
     7141 tgcgatgacg cttcgttcca gacctgctcc gacgaccagc gcggccgcgg aaagcatgcc
     7201 gtcgtcttcg gaaagtccca ttacctcgac gccgtcgcgg cgcgccacca tggtgaatcc
     7261 atgcctcgaa agctcaagtt ccccaagtcc gttgagaatc aggaacgaat cgccttcctc
     7321 ggtcatgtgg aacttctttc cgccgtcgca accggccagc agtgttacca ataatgaagc
     7381 ggcacaaagt aagcggacgc cattcatttt gccttgttcc cccggcaagc cagacccggg
     7441 attatattcc gaatgcttgt ggttgctgta taatcggcca gtttgtttat gacgccatgt
     7501 cgtcccgggg gatcgattgc caccaccttc ccttaagatc gtcattgccg gagccggggc
     7561 catcggcggt tacctggtgt cgcagttttc gggccagggc catcaggttt caatcatcga
     7621 cgtcaattcg gatcgattcg atcgattccc gtccgtgccg cgtgaacgca tgtacgtcgg
     7681 gaacctggtg tcaagttcgg ttcttgcgcg ggctggtgcc ggctccgccg atatcttcat
     7741 cgcgtcttca aaccagcacg aggcaaacgc gctttcggcg atgaaggcca ggcgcatggg
     7801 ggcggccagg gttgtcgccc tgattgaaaa ggccgactat ttcgacgatc cggtcggtat
     7861 ttatcgcgac tggttcggga ttgacctggt tctgaatacc aggttcctgg tggccgggga
     7921 aatcgccaag gtcatcagga cccgtggcgt gctggccgtg gatgccttcg tcgaacacgg
     7981 ttttgagatg gttcagttcc gggttcaggc ggaaaccgag cataccgggc gaccgctgcg
     8041 tgatttgcgt ctgcctgaac agacactcgt ggtcgctctt aatcgagacg gcgtcctgac
     8101 gattccccgc ggtgacgatt cggtgcgcgc cggcgacgag gttctggttc tttcaagcgt
     8161 cgacaagatt ccttttgtgg aaaggatttt cgggcgcagc aaggtcgcag gccacaagac
     8221 cgtgattctt ggcggcggaa cggtcggcca cgcgctggca cgccagattt cgggaagggc
     8281 ttccaacata accgtgattg aacgtgatct ggatcgctgt gatttccttt ccagggaact
     8341 cgatcgtaca acggtcatct acggcgacgg cagcgacggc gacctgctga acgaggaagg
     8401 cgtcggaagc gccgaggtgt tcgccgccgt gaccggcgac gacgagaaga atattattgc
     8461 agcgacgctg gcaagggcat tgggggcgga acgatg
//
LOCUS       DAOPPA010000079         8437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9398_length_8437_cov_9.783822, whole genome shotgun sequence.
ACCESSION   DAOPPA010000079 DAOPPA010000000
VERSION     DAOPPA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9398_length_8437_cov_9.783822"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1980..2510)
                     /locus_tag="PKH54_03750"
                     /pseudo
     CDS             complement(1980..2510)
                     /locus_tag="PKH54_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2653..2844)
                     /locus_tag="PKH54_03755"
     CDS             complement(2653..2844)
                     /locus_tag="PKH54_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99033.1"
                     /translation="MLLTYIKATLLLLMANLIAALGWIDFLIARLETLVGIFASSPAL
                     IILFLMISLLAADLVVRSN"
     gene            complement(2879..3511)
                     /locus_tag="PKH54_03760"
     CDS             complement(2879..3511)
                     /locus_tag="PKH54_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99034.1"
                     /translation="MTENRDTTNNREADARPTNILVDHDHISLDFDGLRLVGLIERFS
                     WHVVLVRLVAPIDLPERISREIKYHCPAPEQRFSLFDEAGRELPKLSPLGIESAANLV
                     VNLWIASRTALTYRDRIRAVYEPIKAGLALAQERDREGRAELRRGLREGRLSPQRVQD
                     GSKRQTKATNQAISHYNNDMTRVWQELAPIMPPSVHPELVIRCWNALEHD"
     gene            4148..5860
                     /locus_tag="PKH54_03765"
     CDS             4148..5860
                     /locus_tag="PKH54_03765"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC99035.1"
                     /translation="MKRDICVQRAGFTRPWAGRCVWRAALSSSDEFRLEDIPELDPDP
                     DSDDGGPDDVRTAPVPAQVELPPDVRDVPRTAHETDTRPDARFRWDGAVGTWVNPDGS
                     PVSPADFFRGWSVVSLLGIHGCRFIEDHILAGLALGEPVLLIGAPGGAKTAMTERIAA
                     DMQMRFWAYDASKAMFEDIVGFPDPASLSRGEVRYVPTPLSLQGKQFILVDEVSRAHP
                     ALQNKWLEIIRSRRVMGLELPEMQHVFGAMNPAGLAGTVPLDEALAGRFTLHIAVPDI
                     NTMSDVDRRAVIESGDRNPGLPDGQASRIRWIVDAVRSAMPAVESEFGIELTTYVAEL
                     SRYLAGKDWRLDGRRQGMIRRGLAAYVAVRKVFMHGAIPRSALGRVLRQGLDALMPFG
                     AMGRELPRIVLDGANQYAVSAMGGNARTLPPSDFLIAAEMVARGRVSADADQMSLLVT
                     RILSSMDRSGRPEDQLRAAAAMLTLALSPQAMFCLPIETRQRVMVAFSDSLELESEDI
                     GSYLNEAFKTTIEGLDPKIQEPVMRVAFNLRNRRSRHRGQSMDFEQMLQILSRFMMEG
                     GAAC"
     gene            5854..6972
                     /locus_tag="PKH54_03770"
     CDS             5854..6972
                     /locus_tag="PKH54_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99036.1"
                     /translation="MLMKALRKVLFDQVVIGMVVDDVRVPIELVTEGRMNSLEWGEVQ
                     TDCLSMRLSMKDVSSRWLPMCLDSIAGSDFGRPGLLIGRTSAVALEYLVEHWVCGTKQ
                     KIDQTVQAARTARSRGEPFLHLVQSFSTHDGRGHRHRDIEEESAEEFIWTKEQFQSAF
                     SRAHDTMRSVYTPDRGPFDIDMMQAVVVPQRRVVGGQVIQGWDVMGLPDDRDSRSVMV
                     SCDGGWFVGPYRRVDPRIMSRFERHGRLVGIRNADMTVWQNWVAKILVKIPHNSTAVG
                     NDTDPECLTGIPVGKLVDARIPVNFAPDIKVVKDPAMALDILCRSARGYVMSGMGGDC
                     LPPSFKKLAGRWLAVCVPVQWMDMGPCDIGDTVFRGAA"
     gene            7000..8382
                     /locus_tag="PKH54_03775"
     CDS             7000..8382
                     /locus_tag="PKH54_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99037.1"
                     /translation="MTDSQSDKLQERVWQAIEFSADPGALAQAMHEFMPQCTFGVSDK
                     VQTACISRSADGSMKLEFGRDFLDRELADDRDFLFVLMHEIYHHVLGHLFPSRKDRVS
                     RIYRNMANVAADIMVNRAVCQRFFPDGVPLLNRMYRTDRLPEALLRPPDFDMEAVRYH
                     QVYREQMIAQFKRGLARMGADKRLAGKVWLLCRAAWCKNAPYGFILERLMAIFSDTDA
                     LRLPRFVLIGSHQGEFGGLGDIIGIGDGGKGAGYSEDVETGETQVQKAAVNYGVAAAL
                     RRALEANSNRWQLNDPVACPGVVCVPGRRDAAMLAAGSYPVFYSANKASLEPEQLAHV
                     YVDVSGSMQDEMPYMFGVLSMSRELIADPIHEFSNMIEDVSLHDFSRGVCRTTGGTDF
                     DVIMKHAMDRSFRQVIIFTDGWASHCESVYERFKAAGKKLHLVFVNSSSESRNECTLT
                     PIATSIFVMS"
BASE COUNT         1678 a         2467 c         2502 g         1790 t
ORIGIN      
        1 ccccggccat actcgtccca tggaggcgtt ttgtggatga aagttcctac acgcctaccg
       61 cacgcatcgg tagtatctgt ccgggtagaa agaatccaca ttatcgaccg acccggttga
      121 gaaagttaca gcccacatgc ccgcgttacc gtccaccttt gatgaagacc agtaggtccc
      181 gcactctcca tgcagtccgc ttagccagag gcaagcaggc tcatagaagc aaccaccgca
      241 attgctgccg tcgcttgctc tgcaggctag agtcagacaa ctgtcggtga ccccacagat
      301 tccgccatcc tcagtaatcg gacagccgcg aatcaaggtt cgaagttctc cgatggatgg
      361 caggcgccaa ccccctccgt cgagaccaag attcgagcaa atgctttttg cggacgcact
      421 tccgactgtt tcgccggagg gctggtacca ctccaggccg gaagtggagt cgatcaaggg
      481 cgcccactcg catcgtcccg gccaacagac ttgaccggaa gggcaggttc cacagatgcc
      541 gccacaacca tcgttgccgc attccctatc atcgcatgca gtgaagacgc attgacccga
      601 ccggcattcc tctccgcata tacaactccc acaggccccc ccacaaccat ccgttccgca
      661 ctctttattg taacaggcgg aaaagacgca ttttcccgac tgacaggttt ccccacatcc
      721 ggagcaactc ccgcaacttc ctccgcagcc atcatccccg cactccttac cgtcgcagtg
      781 gggtgtgcag cacctaccca ggtagcaggt gtcactgccg acgcaactgc cgcacgtgcc
      841 accgcatgta tccgtcccgc agtttctgcc gacgcatgcg gtgaatacac acatgccgga
      901 ctcgcaggtt tccccacacc cggagcaact tccgcaactt ccgccgcagc catcatcccc
      961 gcactcgttg acgtcgcaag ctggggtaca gcacaggccc atgttgcaga cgtcagtgtc
     1021 tgcgcaactg ccgcacgtgc caccgcatgt atccgtcccg cagtttctgc cgacgcatgc
     1081 ggtgaataca cacatgccgg actcgcaggt ttccccacac ccggagcaac ttccgcaact
     1141 tccgccgcag ccatcatccc cgcactcgtt gacgtcgcag tcgggtgtgc aacagaccgc
     1201 gtggtagcag acactgcctt caacgcacga gcagtcggag ggacacgact cgcaggtctc
     1261 atctaagctc gtgtcgcaaa tctcgtcagc acagtagggt tgataaacgc aggttccatc
     1321 gtcgcactgg tagtgctcat tgcatttacc gcaggacgaa ccgcaggaat cgtcgccaca
     1381 ggttttgtcc tcacaactac tgaaacactg acaggttccg tttacacagg ccgcggggac
     1441 aatgcagcca tctgggcatg accggttgtt ggaacaaaca tagaaggctc cggccttgat
     1501 acattctgca tcttcgccgc aatcgccgtt acctggagtg catgcgaagg tttgttcatc
     1561 ggaggggtgg tcgctgtagc aaccgcacac agcgagcagg ctgacgacaa gaaatgcaaa
     1621 agctgacttc acgtagaaat tcatcaaaac gccctagcta ttggaatgtc cccatccggt
     1681 tagacacaag tctggccctg taaaggtccg tagtcaacga catgattcga cctcatgcta
     1741 ccttcgtgag cgggatgccg agattagcgc accggaccag ccatcagaag cccctatcct
     1801 acctaccatc aattttggcg caaattagcc cgattcggga tggcaatcaa ccgacaatgt
     1861 agttcctgga ctgtaaagtg ggtggtgtgc cttttgcgga tggggtatat tgcgggccag
     1921 cccgatattt tcgcgtgtcg gaacctgtca acaagtttga gacacatgcc gtccacaatt
     1981 taagccaact ccggtactgc cgcatccgtg gattccgggg gcgggttaat ccaggcgatg
     2041 gtcggcaaag cctgaagccg gggcattccc tttgtaaaac gctctggatg ggccagccag
     2101 gcggccatcg tcacctgatg cctgcgccgt aagacctcat tcggattacc gtaatgtacc
     2161 tgcgccggcg tcagcattgc aatgccgctg tgccggtgtt cgttgttgta ccagttgaag
     2221 aaatcgccac agaaggccct ggcgtcctgt atggacccga aattgccagg gaattccgga
     2281 cagtatttca gggtcttaaa ctggctttcg gagtacggat tgtcgttgga aacccgtggc
     2341 cggctgtgtg acttgtcgat gccgaggtcg tccagcaggt ttgcgacgac cttcgccgtc
     2401 atcggggacc cgcgatctgc gtggatgtcc agttgcctgg gttcgatgcc ttgtttttcg
     2461 caagtggccg aaatcaagcg ctttgctcgt tccgattccc gactttgaca gccgctggaa
     2521 ccccgaccat actcgtctca aggagccgtt ttgtggatga aagttcctac acgcaatacc
     2581 ggcgagtgat ctgttttttt aacccggtga tcgccataag caacgcctgc ttcggcctga
     2641 ttccagacga cgtcaatttg accggacaac aaggtcagcg gccagcagcg agatcataag
     2701 aaacaggatg atcagggccg ggctggacgc gaaaatgccg accagggttt caaggcgtgc
     2761 gattaagaaa tcaatccagc ccaacgctgc aatcaggttg gccatcaaca gcagcaacgt
     2821 ggccttgatg tatgtcagca gcatcctggt ccccttcggg tgggcacggt caaggtcatc
     2881 agtcgtgttc aagggcgttc cagcacctga tgacaagttc cgggtgaacc gatggcggca
     2941 ttatcggcgc aagttcctgc cagacccggg tcatatcatt gttgtagtgg cttattgcct
     3001 ggttggttgc cttggtttga cgctttgagc cgtcctggac gcgttgcggg gacaggcgcc
     3061 cctcacgcaa tcccctgcgc aattctgccc ggccctcgcg gtcgcgctcc tgcgccagcg
     3121 ccagcccggc cttgatcggt tcgtaaacgg cgcgaatccg atcgcggtag gtcagtgcgg
     3181 ttcgactggc aatccacaga ttaactacca ggttcgctgc agactcgatg cccagcggcg
     3241 agagcttcgg cagctcgcgc ccggcttcgt caaacagcga aaaacgctgt tccggcgcgg
     3301 gacaatggta tttgatttcc cgtgaaattc tttccggcag atctatcggg gcgaccaggc
     3361 ggaccagcac gacgtgccat gaaaagcgtt cgataaggcc gaccaggcgg aggccatcga
     3421 aatccaggct gatgtggtcg tgatcgacta agatgttggt cggtcgggcg tcggcttccc
     3481 ggttattggt tgtgtcccga ttttcagtca tagcattacc ccacactttg acgcatacat
     3541 aaatatgcaa tccaattttc tatgttccat gtcggatatt gtggatcgaa accgggaaaa
     3601 tgtcaagcgt tttctgtgtt tcctggggca actggcgact ttgcggcgcc tcgcacgcgt
     3661 ttcattgtgt gcatagttcg ccagcctttc atcagggcgg ccggaaaggg gcccaatgcc
     3721 tggcttgaaa aaacagcttg acaatacgat gggttagtgc tagtctccac ccacacttcg
     3781 gcaacaagcg gtctgtcggt acgggtcgcg ataaacagga atcaggctat gtgggttgat
     3841 ctctccaggg gctcgtctat cggggatgct tccgtggatc gtctgtgtag tttatttgac
     3901 ccccggaagc agatttcttt gaatcaggcc tggtcagttt gtataagtac aagcaacccc
     3961 cgaaaaagat cggaaaagtt gcaaaacggc gggaatcttg ccggcaggtc ggttattgtc
     4021 tgcggtatgt tgggattcat tctggcaacc tgcttgctgc ttccgacccg ggccgggttc
     4081 aaacagaacg gccgggtcca gccgggccga cagaccgaca tcgtataaat tttcaggagg
     4141 tgtagatatg aaaagggaca tttgtgtcca acgggccggt ttcacccggc catgggcagg
     4201 ccgctgcgtc tggcgtgccg ccctgtcgtc aagtgacgaa ttccgactgg aggacatccc
     4261 ggaactcgac cctgaccctg attccgacga cggcggacca gacgacgttc ggactgctcc
     4321 ggttcccgcc caggtcgaac ttccaccgga cgtgcgtgac gtcccgcgta ccgcgcatga
     4381 aaccgatacc aggccggacg cccgattccg ctgggacggc gccgtcggaa catgggtcaa
     4441 cccggacggc tcgcccgtca gcccagccga tttcttccgg ggatggtcgg tcgtgtccct
     4501 gctgggtatt cacggatgcc ggttcatcga ggaccacatc ctggccggtc ttgccctggg
     4561 cgagcccgtg ctgctgatcg gcgcgcccgg cggtgccaag accgcaatga ccgagcgtat
     4621 cgcggccgat atgcagatgc gtttctgggc atacgacgcg tccaaggcga tgttcgagga
     4681 catcgtcggg ttcccggatc cggccagcct ttcccgtggc gaggtccgtt acgtcccgac
     4741 accactgtcc ctgcagggca agcaattcat cctggtggac gaggtcagcc gcgcccaccc
     4801 ggcgctgcag aacaagtggc tggaaatcat ccggtcgcgc cgcgtgatgg gtttggaact
     4861 gcccgaaatg cagcacgtct ttggcgccat gaacccggcc gggctggccg ggacggtgcc
     4921 gctggacgaa gcgctggctg gccggttcac gcttcacatc gccgtgccgg acatcaatac
     4981 gatgtctgac gtggaccggc gtgccgtcat cgaatccggc gatcgcaatc cgggccttcc
     5041 ggacggccag gcttcccgga tccgctggat cgttgatgca gtcaggtcgg ccatgcctgc
     5101 tgtcgagtca gaattcggca tcgagctgac gacctatgtc gccgagctat cccgttacct
     5161 ggccggcaag gactggcgtc ttgatggacg ccgtcagggc atgatccgcc gcgggcttgc
     5221 cgcctatgtg gcggtcagga aggtcttcat gcatggggcg atcccgcgat ccgcccttgg
     5281 acgcgtgctt cgtcagggcc tcgacgcgtt gatgcctttt ggcgcgatgg ggcgcgaact
     5341 gcccaggatt gttcttgacg gcgccaacca gtacgcggtg tcggcaatgg gaggcaacgc
     5401 caggaccctt ccgccgtccg actttctgat tgcggccgag atggttgcaa ggggccgtgt
     5461 cagcgccgat gccgaccaga tgtccctgct ggtcaccagg attctgtctt cgatggaccg
     5521 ttccggcaga cccgaagatc agctccgggc cgcggcggcg atgctgactc tcgccctgtc
     5581 gccccaggcc atgttctgcc tgccgatcga aacccgtcag cgcgtgatgg tcgcgttttc
     5641 cgactccctc gagcttgagt ccgaagatat cggctcatac ctgaacgagg ccttcaagac
     5701 caccatcgag ggtcttgatc cgaagatcca ggaaccggtt atgcgtgtgg cgttcaacct
     5761 gagaaaccgt cgttcgcgcc atcgcggcca gtcgatggat tttgagcaga tgctccagat
     5821 actgtcgcgt ttcatgatgg aaggaggtgc cgcatgttga tgaaggcgtt aaggaaagtg
     5881 ctgtttgacc aggtcgtcat cgggatggtg gtcgacgacg ttcgggtgcc gattgagctg
     5941 gtcaccgaag gacgcatgaa ctcgctggag tggggcgagg tccagacgga ctgcctgagc
     6001 atgcggcttt cgatgaagga cgtgtcttcg cggtggctgc cgatgtgtct ggactccatc
     6061 gcgggttccg atttcggccg accgggtctg ctgatcgggc gcacttcggc agtcgccttg
     6121 gaatacctgg tggaacactg ggtctgcggc acgaaacaga agatcgatca gactgtccag
     6181 gccgccagga ccgcacgctc caggggcgag ccgttcctgc acctggtaca gtctttttcc
     6241 acccacgacg gacgcggtca ccgccacagg gatatcgagg aggagtccgc cgaggagttc
     6301 atctggacca aggaacagtt ccagtccgcc ttcagccggg cccatgacac aatgcggtcc
     6361 gtttacacgc cagaccgcgg cccattcgac atcgacatga tgcaggccgt ggtcgttccg
     6421 caaaggcggg tggtcggcgg ccaggtgatt cagggctggg acgtgatggg actgccggac
     6481 gaccgcgatt cccgcagcgt catggtttcc tgcgacgggg gttggtttgt cggtccttac
     6541 cgtcgcgtcg atccccggat catgtcccgg ttcgaaaggc acgggcgcct ggtcggcatc
     6601 cggaacgcgg acatgaccgt atggcagaac tgggttgcca aaattctggt caagattccg
     6661 cacaattcga cggctgtggg caatgacacc gaccctgagt gtctaaccgg gattccggtc
     6721 gggaagctgg ttgatgcaag gattcccgtc aacttcgccc cggacatcaa ggttgtgaag
     6781 gaccctgcca tggccctgga catcctttgc aggtccgcac gcggatatgt gatgtcgggt
     6841 atgggcgggg actgtcttcc cccgtcgttc aagaagcttg ccggtcgctg gctggccgtc
     6901 tgcgtgcccg tccagtggat ggacatgggc ccgtgcgaca tcggggacac ggtgttcagg
     6961 ggtgcggcgt gatttgatgg aaccatgcag ggggaatgta tgaccgattc gcaatccgac
     7021 aaactgcagg aacgtgtatg gcaggcgata gagttttctg ccgaccctgg tgcattggcc
     7081 caggccatgc acgaattcat gccccaatgc accttcggtg tgtccgacaa ggtccagacg
     7141 gcctgcatca gccggtcggc cgacggaagc atgaagctgg aattcggccg ggactttctg
     7201 gaccgggaac tggctgacga cagggatttt cttttcgtcc tgatgcacga gatctatcac
     7261 cacgtcctgg ggcacctttt tcccagcagg aaggatcggg tttcaaggat ctaccggaac
     7321 atggcaaacg tggcggcaga catcatggtc aaccgggccg tgtgccagcg gttctttcct
     7381 gacggcgttc cgctgctgaa ccgtatgtat cgaacggatc ggctgcccga ggccttgctg
     7441 cgaccaccgg atttcgacat ggaggctgta cgctatcatc aggtttaccg cgaacagatg
     7501 attgcccagt tcaagcgcgg tcttgccagg atgggcgcgg acaagaggct ggccggcaag
     7561 gtttggttgc tgtgtcgggc ggcctggtgc aagaacgccc catacgggtt catccttgag
     7621 cggctgatgg cgatcttctc ggataccgac gcgctgcggc tgccccgatt cgtcctgatt
     7681 ggaagccatc aaggtgaatt tggcggcctg ggcgacatca tcggaatagg tgacggtgga
     7741 aagggagccg gctattccga ggatgtggaa acaggggaga cccaggtaca gaaggcggcc
     7801 gtgaactatg gtgtcgcggc ggcattgcgg cgggcgctgg aagccaattc gaaccggtgg
     7861 cagttgaacg atcccgtcgc atgccccggg gtcgtctgcg tccccggccg gcgtgatgcg
     7921 gccatgcttg cggccggttc ataccctgtg ttctattcgg ccaacaaggc atcgctggag
     7981 cccgaacagc ttgcgcacgt ctatgtcgat gtctccgggt cgatgcagga tgaaatgccg
     8041 tacatgttcg gggtcctttc aatgtccagg gaactgattg cggacccgat tcacgagttt
     8101 tccaacatga tcgaggacgt cagcctgcac gatttcagcc gtggcgtctg ccgcacgacg
     8161 gggggcaccg actttgacgt gatcatgaag cacgcgatgg acaggagctt ccggcaggtc
     8221 atcattttta ccgatggctg ggccagccat tgcgaatctg tctatgagcg cttcaaggcg
     8281 gccggcaaaa aactgcacct ggtgttcgtg aattcgtcga gcgaatccag gaacgaatgt
     8341 acgctgaccc cgattgcgac cagtatcttc gtgatgtcgt aattaatccg gagttccgcc
     8401 gcgatttgag tgctatcttc ctgtaatact tcagtta
//
LOCUS       DAOPPA010000080         8421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9423_length_8421_cov_4.102678, whole genome shotgun sequence.
ACCESSION   DAOPPA010000080 DAOPPA010000000
VERSION     DAOPPA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_8421_cov_4.102678"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKH54_03780"
     CDS             complement(<1..500)
                     /locus_tag="PKH54_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99038.1"
                     /translation="MKTTSMVRADTRGDMPTPGESRVGNRLHICLGFSCNNNCLFCME
                     EDREARFQRLSNQTAEDVRRMMTADPDAREVMFTSGEPTLHPQLPEYLEMARDLGFPT
                     VGMITNGRRLSNRAYARKLLESGLNHILISIHGPDAHVHDGLTRTKGSFNQALVGLAN
                     LLLLKR"
     gene            819..1979
                     /locus_tag="PKH54_03785"
     CDS             819..1979
                     /locus_tag="PKH54_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOC99039.1"
                     /translation="MIVGVPREIMPGERRVAMTPSSVKSYIDFGFEVLVESGAGAGIF
                     ATDAEYAAEGAKIVHDVQELFSRADIILKVKQPEFNHATGKNEIEMMPEGRMLVTFLH
                     PANPESHANVRLLRDRRILAFTMDGIPRISRAQRMDALTSMSTCTGYLSVVDAARHLP
                     RFVPMMTTAIGMIKPAHVLVIGCGVVGLQAVATAKRLGAITHVFDIRDAAKTEGGSLG
                     AKVAGFDIPQDIALAPGGYALPLSEEWLQKERAALAPIIKDMDIVILCALVFGQKAPI
                     LVTEEMVKSMKPGSVIVDVSIDQGGNCAVTAPGKEIDVNGVQVCGVQNIPGRLPVHST
                     WLYSQNMFHYISNMFKNSKTEPDYSDEIVQSSLVTKDGKILHQGTIKAMGEV"
     gene            1983..2309
                     /locus_tag="PKH54_03790"
     CDS             1983..2309
                     /locus_tag="PKH54_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOC99040.1"
                     /translation="MGTEAAAVEAITGVEMLIMAGVFVVASAVGYFLISRVPSLIHTP
                     LMSMTNALSAVTILGGILLFSKEVGTIELVLGAVALVAASFNLFGGYMVSHRMLKMFK
                     TKKALS"
     gene            2353..3855
                     /locus_tag="PKH54_03795"
     CDS             2353..3855
                     /locus_tag="PKH54_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOC99041.1"
                     /translation="MFSGIDIPDSVVLVIELAIIGLLLLGISKFRNPPGARFGNHAAL
                     ISILAAMALVLVTDGSRQPVLLAVVVAVGLVLGFVSAFKIPMTQMPSMIAIQNGMGGL
                     ASLMISFAELGFSRTPVSGLSGVGGFIGVVLGAFTLSGSIVAAAKLNNHLKNQGRSPA
                     INPILVVIALAVAVACVFAWNVSGSLVMIILVGLVVLSLAMGYLFAIRIGGADMPVVI
                     SFLNSASGLAAAFTGLALNSRLLVVCGATVGSSGFILTAAMCKAMNRSLLNIFVGTTV
                     ASKGCGEVKSAEQAVSDEPAVEDSAVEQGAADACAAVAAIEPEVELTFEQKITKVACA
                     ARDAETVVFIPGYGMALAQAQFAVIALANALEKMGKNVVFAIHPVAGRMPGHMNVLLA
                     EADVDYEKLIEMDEINPKFATTDLVFVVGACDVVNSAAIHKEGTPISGMPVLLAHEAK
                     SVVVCNLDTRPGYSGVENPLYEDPRTVMLLGDAKATVEMITAAVSGTGAA"
     gene            3962..5032
                     /locus_tag="PKH54_03800"
     CDS             3962..5032
                     /locus_tag="PKH54_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOC99042.1"
                     /translation="MEIELEKKPVSRLVPTDIVFQTPFWAAVKSSLGWRTLAFDFSFA
                     GKEGDVLLLQRQMADHGNLVYVPQGPEALPDEEARGVFLESLANHVRGILGDSTRIVR
                     FDLPWRSPYQDVAETSGLTDYRPETRVREMRMNFGTQTWNLRAAPSDVFAPVTMVIDL
                     APDLDDILSGMKPKTRYNIRLSERRGVVVKRMPVEFLPAFHRMYVQTASRDGFPVPSI
                     EHLEALFRAADPAIDDVDVQMFCAFKDDELLAGAIIAFSEKCAIYLHGASYAMMRDHM
                     ASYAVQWAVMTYAKAYGCTWYDMFGVSPQGYEGHPLSGVRRFKSGFGGHIVQREGTWD
                     YPFDPDLYSGFTFGELLADFST"
     gene            complement(5166..6371)
                     /locus_tag="PKH54_03805"
     CDS             complement(5166..6371)
                     /locus_tag="PKH54_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC99043.1"
                     /translation="MGETGQRRDTILGFNRNIFFVGLVSLLTDTSTKMVYSIMPMFLM
                     SIGASKTTLSVIEGIAESTAALVKTAAGFFSDRIGRNKPFMVVGYAITAIVTPIYAAV
                     TSPVQVLALRFVERFGKGVRTAPRDSLVAGSATSGNLGRSFGLHKAMDNSGAIIGPLI
                     AFGLIAALGENYRMIFILSAIPAVLGVLTIIGLIRDAKKPAESLYRKFRFRDFPKKFY
                     LLLAIIFLFTLGNSTDALLIVKANEVGIKIAYIPLVYLIFNAVSVLLAIPIGSISDKV
                     GRERILVFGYLVYAVVYLGFGYTTDVRVIVGLFATYGLYSACTDGIQKAFVSEMLDKN
                     KQGTGLGIYNALLGITLLPASIIAGVLYDKVNNRVPFYFGAATAILAAVLMIVFAITN
                     RRRPATTTD"
     gene            6524..>8421
                     /locus_tag="PKH54_03810"
     CDS             6524..>8421
                     /locus_tag="PKH54_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOC99044.1"
                     /translation="MRMDRFTTKAREAIEAAARSATERGNPEIDVEHLLLALLSEKEG
                     VVASVLKLMEKSPDAIASRMQGAVEGLPRVSGGGQIGVSRGLQDLFTAAEKEAAGFKD
                     DFISSEHLFLAALAARDGHAVRLLREAGIERSSFLLALKEVRGNVRVTDEDAENRYQS
                     LQRFSIDLIQRARQGKLDPVIGRDAEIRRVIQVLSRRRKNNPVLVGEAGVGKTAIVEG
                     LARRIVQGDVPENLKNKKLVALDMGLLIAGAKYRGEFEDRLKAVLKEVEESDGEIILF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGTLHAIGATTLKEYRKYIEKDAALERRFQ
                     PVQVDAPSVADTITILRGLRERYEVHHGVRIREAAIIAAATLSDRYIPDRFLPDKAID
                     LVDEAASRLRMEIDSMPVELDVLERRLAQLEIERQAIGREECPTDGGRCGRIEAEMAD
                     IREQLSALRAHWQKEKDLVGEIRDLKRALEEEGALEEAAVRRGDWEAASKLKYGSIPE
                     KQAKIAELSGKLAEIQADRKMLREEVDDEDIARVVSAWTGIPVSKMLEGEQKKLLQME
                     ERLARRVVHQDQAISVIANAVRRSRAGLSDAARPIGSFLFLGPTGVGKTELVKALAEF
                     LFDDETAMV"
BASE COUNT         1655 a         2323 c         2717 g         1726 t
ORIGIN      
        1 tcccttttca gcaggagcag gttggccagt ccgacaaggg cctgattgaa cgaacccttt
       61 gtacgtgtca gtccgtcatg gacatgcgcg tccggcccgt gaatcgaaat aaggatgtgg
      121 ttcagcccgg actcaagcag cttgcgtgcg tacgcacggt tggaaagccg tcggccgttg
      181 gtgatcatcc cgaccgtcgg aaatccaagg tcacgcgcca tctccagata ctcgggaagt
      241 tgcgggtgca gggtcggctc gcccgaggtg aacatgactt cacgcgcgtc ggggtcggca
      301 gtcatcatcc tgcggacatc ttcggccgtc tggtttgaca gacgctgaaa gcgggcctcg
      361 cggtcttctt ccatgcagaa aaggcagttg ttgttgcagg aaaatcccag gcagatgtgc
      421 aggcggttgc ccacccttga ttcgccgggt gtcggcatgt cgccgcgtgt gtcggcacgt
      481 accatcgaag tcgtcttcat ccagtcccgg ccaaggatta taggtgttct gggcacaatt
      541 acaattgaac tggcttcttc ggttcgggat tcgcgatgtt cggctgattg acggagtcat
      601 tgatttttct gttggataaa gtggcttcaa agtgctttgg ctattgcatt aatgggcagt
      661 ctttggtatc gacggatgcg gaaatccagc ccgacgcgac gacggatttt tcgtcaggga
      721 tgccgcggga tgggcctgga ggatcaccgg gggcgctttc cttgttccaa agcgccctgg
      781 aaccgtgaat tgcagtggtt ccaagcagga gtatcaaaat gatagttggt gtacctcggg
      841 agattatgcc tggcgaacgt cgtgttgcga tgactccttc cagcgtgaag tcatacattg
      901 atttcgggtt cgaagtgctt gtcgaaagcg gtgccggtgc cggcatcttc gccaccgacg
      961 cggaatacgc ggccgaaggc gcgaagatcg ttcacgatgt gcaggaactg ttttccaggg
     1021 cggatatcat cctgaaggtc aagcagcccg aattcaacca tgcaaccggc aagaacgaga
     1081 tcgagatgat gccggaaggt cgcatgcttg ttaccttcct tcatccggca aatcctgaaa
     1141 gccacgccaa cgtgcgcctt ctgcgggatc gcaggattct tgctttcacg atggacggga
     1201 ttccccgtat ttcccgcgcc cagcgcatgg acgcccttac gtcgatgagc acgtgtaccg
     1261 ggtacctttc ggtcgtcgac gcggcccgcc acctgcccag gtttgtcccc atgatgacga
     1321 cggccatcgg catgatcaag ccggcccacg tcctggtcat cggatgcggc gtcgtcggtc
     1381 tgcaggcggt tgccacggca aagcgtcttg gcgcgattac ccacgtattt gatatccgcg
     1441 acgcggcgaa gaccgaaggc ggcagccttg gcgcgaaggt tgccgggttc gacatcccgc
     1501 aggacatcgc ccttgccccg ggcggctatg cgcttcccct ttccgaagaa tggcttcaga
     1561 aggaacgtgc ggcgcttgcc ccgattatca aggacatgga catcgtgatc ctgtgcgccc
     1621 tggtgttcgg tcagaaggcg ccgatactgg tcaccgaaga aatggtcaag tcgatgaagc
     1681 ccggttctgt catcgtcgat gtttcgatcg atcagggcgg aaactgcgcg gtcacggccc
     1741 ccggcaagga aatcgatgtc aacggcgttc aggtatgcgg cgtccagaac atccccggcc
     1801 gtctgcccgt ccattcgacg tggctgtatt cgcagaacat gttccattac atctcgaaca
     1861 tgttcaagaa cagcaagacc gaaccggatt actccgacga aatcgtccag agttcccttg
     1921 tgacgaagga tggcaagatt cttcatcagg gcaccatcaa ggcgatgggg gaggtttgat
     1981 tcatgggaac tgaagccgct gcagtcgagg ccatcaccgg cgtcgaaatg ctgataatgg
     2041 ccggcgtctt cgtcgtggcc agcgcggtcg gatatttcct gatttcgcgc gtgccgtcgc
     2101 tgattcacac gccgctgatg tcgatgacga acgcgttgtc ggcagtgaca atcctgggtg
     2161 gtatcctgct gttctcgaag gaggtcggca caatcgaact ggttcttggc gcggtcgccc
     2221 ttgttgccgc gtcgttcaac cttttcggcg gatacatggt ttcccacagg atgctgaaga
     2281 tgttcaagac gaagaaggct ttgtcctgac tcgggcttcg accccgggcc agtccgggcg
     2341 gcggaggttc tcatgttttc cggtatcgac attcctgaca gtgtggttct ggtaatagaa
     2401 ctcgcgatca tcggcctgct tctgcttggg atttcgaaat ttcgcaatcc tcccggtgca
     2461 aggttcggga atcacgcggc gctgatttcg attctggctg ccatggcgct tgtcctggtt
     2521 accgacggtt cgcgtcagcc ggtgttgctg gctgtcgtgg ttgcggtcgg cctggtgctt
     2581 ggatttgtca gcgccttcaa gatcccgatg acccagatgc cgtcgatgat agccatccag
     2641 aacggcatgg gcggccttgc ttcgctgatg atttcctttg cggaactggg attctccagg
     2701 actcccgtca gcggcctgag cggcgtgggc ggttttatag gcgtcgttct gggcgcgttc
     2761 actttgtcgg gcagtatcgt tgctgcggcc aagttgaaca accacctgaa gaatcagggc
     2821 aggtcgcccg ccatcaatcc gattctggtc gtgatcgcgc ttgctgtcgc ggttgcctgc
     2881 gtcttcgcgt ggaacgtaag cggcagcctg gtcatgatta ttctggtcgg actggtcgtc
     2941 ctgtcgcttg ccatgggtta tctgttcgca atcaggattg gcggcgccga catgcccgtt
     3001 gtgatctcgt tcctgaactc cgcctccggc ctggcggccg cgttcaccgg tcttgcgctt
     3061 aacagccgtc tgctggtggt ctgcggcgca accgtggggt cgtccggatt catcctgacg
     3121 gcggcaatgt gcaaggccat gaaccgaagc ctgctgaaca ttttcgtggg aacgactgtg
     3181 gcttcgaagg gatgcggcga agtgaagtcg gcggaacagg ccgtttccga cgaaccggcc
     3241 gttgaagatt cggcggtcga acagggtgcg gccgatgcct gcgcggccgt cgcggcgatc
     3301 gaacccgaag tcgagctcac gttcgaacag aagatcacca aggtggcctg tgctgcccgg
     3361 gacgcggaaa cggttgtttt cattccgggc tacgggatgg cactggccca ggcacagttc
     3421 gcggtcatcg ccctggcgaa cgccctggag aagatgggca agaacgtcgt gttcgcgatt
     3481 caccccgttg ccggccgcat gcccgggcac atgaacgtgc tgcttgccga agcggacgtg
     3541 gattacgaaa aactgatcga gatggacgag atcaacccga agttcgcgac gaccgacctg
     3601 gttttcgtcg tcggcgcgtg cgatgtcgtc aattcggcgg ccatccacaa ggaaggaacg
     3661 ccgatttccg gcatgccggt gctgcttgca cacgaagcga agtcggtcgt cgtctgcaac
     3721 cttgacacgc gtcccggtta ttcgggcgtc gaaaatccgc tttatgaaga tccgcggacc
     3781 gtcatgcttc tgggcgatgc caaggccacc gtggaaatga ttactgccgc cgtttcgggg
     3841 actggcgcgg cctagtccca ttcctgccga accgggttgc cgaggttcgg cgcccgggtc
     3901 aggccatttc aacctgatca gaaagccgga cgggcgccga cgatgaaaga aggagtccgc
     3961 catggaaatc gaacttgaaa agaagcccgt cagccgtctt gtacccacgg acatcgtctt
     4021 ccagacgccg ttctgggccg cggtgaaatc cagcctggga tggcgtacgc tggcgtttga
     4081 cttcagcttc gccggaaagg agggcgacgt cctgcttctt caaaggcaga tggcggatca
     4141 tgggaatctg gtttatgtgc cccaaggtcc cgaagcgctt ccggatgagg aggcgagggg
     4201 cgtattcctt gaatcgctgg ccaatcacgt gcgcggcatt ctcggtgatt cgaccaggat
     4261 tgtcaggttt gacctgccat ggagatcacc ttaccaggac gtcgccgaga cgtcggggct
     4321 gaccgattac cgcccggaaa cgcgtgtgcg cgagatgcgg atgaacttcg gcacgcagac
     4381 gtggaatctg cgcgcggccc cgagtgatgt ctttgcgccc gtgacgatgg tgattgacct
     4441 tgcaccggac cttgacgaca tcctgtccgg catgaagccg aagaccaggt acaacatccg
     4501 tctttcggaa aggcgcgggg tggttgtgaa gaggatgccg gtcgagttcc tgccggcatt
     4561 tcaccggatg tatgtccaga cggcctcgcg tgacggcttt cctgttccat cgattgaaca
     4621 cctggaagcg ttgttccggg ccgcggatcc cgccatcgac gacgtcgatg tccagatgtt
     4681 ctgcgcgttc aaggacgacg aactgcttgc gggcgcgatc atcgcgttca gcgagaagtg
     4741 cgcgatttac ctgcacggtg cgtcgtacgc gatgatgcgc gaccacatgg cgtcgtacgc
     4801 ggtgcagtgg gccgtgatga cgtacgcgaa ggcatacggg tgcacctggt acgacatgtt
     4861 cggcgtttcc ccgcagggtt atgaaggaca tccgctgtcc ggggttcgcc gcttcaagag
     4921 cggtttcggg ggccatatcg tgcagcgcga aggcacatgg gactatccct tcgacccgga
     4981 tctgtactcc ggcttcacct tcggcgaact gctggcggat ttttcgacct gaagcaggtg
     5041 gacaaccttc gcgcagttgg ccgcgtacta ctcgatatca ttcaaccttt ctggccgatg
     5101 agaaccattt ccggatgccg ggatgacctt gccgggatcc tggggccggt gcttcccggc
     5161 tgacgtcagt cggttgtggt cgctggcctg cggcggttcg tgattgcgaa gacaatcatc
     5221 aggactgccg cgagaatggc ggtggccgcg ccgaagtaaa acggcacccg gttgttgacc
     5281 ttgtcgtaca gaaccccggc gatgatgctt gcgggcagca gagtgatgcc aagaagcgcg
     5341 ttgtagatgc caaggccggt tccctgcttg tttttgtcca gcatttccga aacgaaagcc
     5401 ttctggatcc cgtcggtgca ggccgaatac aggccatagg tcgcgaaaag accgacgatg
     5461 acccggacgt cggtggtata tccgaaaccc agatagacga ccgcatagac cagataaccg
     5521 aacaccagaa tgcgttcgcg tccgaccttg tccgaaatgg aaccgatcgg aatcgccagc
     5581 agaacggaca cggcgttgaa aatcagataa accagcggaa tatatgcgat tttgatgccg
     5641 acctcgttgg ccttcacaat aagaagggcg tcggtcgagt tgccaagggt gaacaggaag
     5701 atgattgcca gcagcaggta gaatttcttc ggaaagtccc ggaagcggaa tttcctgtaa
     5761 agactttcgg cgggtttttt cgcgtcgcgg ataaggccga tgatggtaag gacgccaagg
     5821 acggccggga ttgctgaaag gatgaaaatc atccggtagt tttcgcccag ggctgcaatc
     5881 agtccgaacg cgatcagcgg cccgattatg gcgccgctgt tgtccatggc cttgtgcagg
     5941 ccgaagctgc gtcccaggtt gccggacgtg gctgaacctg ccacgaggct gtcgcgcggc
     6001 gccgttctga cgcccttgcc gaagcgctcg acaaaccgaa gggcgagcac ctgaactggt
     6061 gatgtgacgg cggcgtaaat tggcgtcacg attgccgtta tcgcatatcc gacaaccatg
     6121 aacggtttgt ttctgccaat cctgtcggaa aagaagcccg ccgcggtctt gaccagggcg
     6181 gcggtacttt ccgcgattcc ctcgatgacc gaaagtgtcg tcttggacgc gccgatcgac
     6241 atcaggaaca tcggcattat cgaatagacc atcttggtcg aggtgtcggt cagcaggctg
     6301 accagcccga cgaagaagat gttccggttg aacccgagga tcgtgtccct gcgctgccca
     6361 gtttcaccca tcgtctgacc ggtgcgtatc cgacccggcg atgttagcac caatcagtcg
     6421 cgtggggcgg gacttgcaca agtgcctgtc caaccaaata gttcaaccgg tcgcggcgta
     6481 cgcgattgcc atacgttaat gacgccgcac cggaggtttg cagatgagga tggacagatt
     6541 tacgacaaag gcccgcgagg ccatcgaggc ggccgcgagg tccgccaccg aacggggaaa
     6601 cccggagata gatgtcgagc acctgctgct tgcgttgctt tccgaaaagg aaggcgtggt
     6661 ggcaagcgtg ctgaagttga tggaaaagtc tccggacgcg atcgcgtcgc ggatgcaggg
     6721 tgcggtcgaa gggctgccaa gggtcagcgg cggcgggcag ataggtgtga gtcgaggctt
     6781 gcaggacctt ttcacggcgg cagaaaagga agcggccgga ttcaaggacg acttcatcag
     6841 cagcgagcac ctgttcctgg cggcgctggc ggcacgtgac gggcatgccg tccggctgct
     6901 gcgcgaggcc ggcatcgagc gttcaagctt cctgctggcc ctgaaggaag tccgcggaaa
     6961 tgtgcgggtc accgacgagg atgccgaaaa caggtaccag tcgctgcagc ggttttcgat
     7021 cgatctgatc cagagggcgc gccagggcaa gctggacccg gtcatcggtc gcgacgccga
     7081 gatccggcgt gtaatccagg tgctgtcccg ccgccgcaag aacaatcctg tgctggtggg
     7141 cgaggccggg gtcggaaaga ccgcgattgt cgaggggctg gcgcgccgga tcgtgcaggg
     7201 cgacgtcccg gagaacctga agaacaagaa actggtcgcc ctggacatgg ggcttctgat
     7261 tgcgggcgcg aaataccgcg gcgagttcga ggacaggttg aaggccgtcc tgaaggaagt
     7321 cgaggaatcg gacggggaaa tcatcctgtt catcgatgaa ttgcacaccc tggttggcgc
     7381 tggcgcggcc gagggcgcca tggacgcttc gaacatgctg aagccggcgc ttgcccgcgg
     7441 gactctgcac gcgattggtg ccacgacgtt gaaggaatac cggaagtaca tcgagaagga
     7501 cgcggccctg gagcgccgtt tccagccggt gcaggtggat gccccgtccg tagccgacac
     7561 aatcaccatc ctgcgcggcc tgcgcgaacg ttacgaggtc caccacggcg tccgcatcag
     7621 ggaggccgcg ataatcgccg ccgcgacgct gtcggatcgg tacatcccgg atcgctttct
     7681 gcccgacaag gcgattgacc ttgttgacga ggcagcaagc cgtctgcgca tggaaatcga
     7741 cagcatgccg gtggaacttg acgtgctgga acgcaggctg gcccagcttg aaatcgagcg
     7801 gcaggccatc gggcgcgagg agtgtcccac cgatggcggg cggtgcggcc ggatcgaggc
     7861 cgagatggct gatatccgcg agcagttgtc ggcgctgcgg gcccactggc agaaggaaaa
     7921 ggatctggtc ggagagatcc gcgacttgaa gagggcattg gaggaagagg gcgcccttga
     7981 agaggcggcg gtacgtcgtg gcgactggga ggccgcgtcc aagctgaagt atggaagcat
     8041 tccggaaaag caggcgaaga tcgcggaact gtccgggaag cttgccgaga tccaggccga
     8101 ccgaaaaatg ctgcgcgagg aggtcgatga cgaggatatc gcgcgggtcg tgtcggcctg
     8161 gactggaata ccggtgtcga agatgctgga aggcgagcag aagaaactgc tgcagatgga
     8221 agagaggctt gcgagacggg tcgtgcacca ggaccaggcc atttccgtga ttgcaaacgc
     8281 ggttcggcgg tcgcgggccg ggctgtccga tgcggcgcgg ccgatcggca gtttcctgtt
     8341 cctgggcccg accggcgtgg gcaagaccga actggtgaag gctctggccg agttcctgtt
     8401 cgacgacgag accgcgatgg t
//
LOCUS       DAOPPA010000081         8420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9424_length_8420_cov_4.125164, whole genome shotgun sequence.
ACCESSION   DAOPPA010000081 DAOPPA010000000
VERSION     DAOPPA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9424_length_8420_cov_4.125164"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKH54_03815"
     CDS             complement(<1..584)
                     /locus_tag="PKH54_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC99045.1"
                     /translation="MAHRIFQLLEKGPVVFDGAMGTLLYSRGIFINRNFDELNVSRPD
                     MVEKVHADYLSAGAMVLTTNTFGANPVRLAGHGLAQRMDEINRAGVRIAREVAGGQAL
                     VAGSIGPSGLIPDLSDEAMIARLTQAFSSQATVLADAGADLLVLETISNPLEMEIILR
                     AVRPVVEIPNIAMAMFKGDAASGEGCCCDGCTPSA"
     gene            complement(665..1174)
                     /locus_tag="PKH54_03820"
     CDS             complement(665..1174)
                     /locus_tag="PKH54_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99046.1"
                     /translation="MKSNRHSRKYPRIMELALTAGKWSVLAGGAVAMIGASGCEQISD
                     DQDAGPDVVQLDGMPYEEDVRIPDPDVHEGTDATFDTGAFVDVPTMGLPPEDYIQPDV
                     VEPDYMGYELSGTDVPWSPDDGADVIDPVEVSELPLSDATEVDPGAPLPGEPPIDVFP
                     GDVIDPDAD"
     gene            complement(1234..2241)
                     /locus_tag="PKH54_03825"
     CDS             complement(1234..2241)
                     /locus_tag="PKH54_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99047.1"
                     /translation="MKNRSILKSAAVAAGVAFLPVASFIAGVWLVPEISLAQSGPEPQ
                     SAVFLVSDRDMKTVGRYEFNQVVREEGVFATSRYFKGAASAKSKKKEAPAVRSYLEMA
                     PDRTFAKYTRWESQGINVREFKIFRYQKDVRMRDALGSKAGVSVLGKWQPVFLVEEDQ
                     PHTAAMIVDRNVPEKTFACINTTLGKIGQATVRIVGVTSVKSVVPDPAPVQAAGSGEP
                     ATQVASPETVVTSPEPAAAAAKPVKPAAETTGRAAATEPTVESAEPASEPATAAAEPA
                     AEPATAAAEPANQVAAPGTADIYEFRVEGDCGVIGVWQDGSGNFVRFSNSLHTFEPVA
                     R"
     gene            complement(2301..3272)
                     /locus_tag="PKH54_03830"
     CDS             complement(2301..3272)
                     /locus_tag="PKH54_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOC99048.1"
                     /translation="MGKILVLVEVQNSRVRKASLSGVTFAADLAGRTSGTFDIAVMGP
                     GARAAAPSLAGFGAGKVHVLESPGLSSYLARPWAGAAASMVEAVGADCLVAVSTTWTR
                     DMLPRVAARLGAGMVSEAVGIDGSGSDVMFRRPMWAGNVIATVRVKTPRCVICVRGTE
                     FSPAEPSGGSSPVVELAVDVAADPAARHVSFEPTVSERPDLAEADVVVSGGRGLGDKA
                     SFWNTLNPLADYLGAAIGATRAVVDNGVAPNDLQVGQTGKAVAPSLYFAVGLSGAIQH
                     LAGMKNSKVIVAINKDEEAPIFSIADYCLVGDALKLVPELVDTLKKS"
     gene            complement(3331..4086)
                     /locus_tag="PKH54_03835"
     CDS             complement(3331..4086)
                     /locus_tag="PKH54_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOC99049.1"
                     /translation="MKILVTAKRVTDPDARIKVLPDGTGIETAGLEYKLNPFCENAIE
                     EAVAIREKTGAEVIAVTIGDEEAVKSLRTALAMGADRGVHVKAGDFELDSDLAARILA
                     AVFAKEGCDLFILGKQAVDGDSNQVGQLVAEYAGVPQACFASRVVIGSDGAVVSREVD
                     GGMETVEVKFPAVITADLRLNEPRLPKLPDVLKAKKKQVDEYAPADLGVDMSMKVETV
                     GYSAPAGRAGGRRVGSVAELVDLLRNEAKVLKA"
     gene            4155..6662
                     /locus_tag="PKH54_03840"
     CDS             4155..6662
                     /locus_tag="PKH54_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOC99050.1"
                     /translation="MFGSVVINNWTKAAMQAVQMMLKHPAAICVILAFLSSACQSFGD
                     VTQDALDQGTVITDAAPDSADLPESDSTGDLPDSGQADIAADGDGADVPTDDGFLPDE
                     TGGDSSVDADVIDPEADSDGDGIPDLDEISDGTDPLDPSDARAWQPGLGPHPRLFFDS
                     DEIPAIAAKLAATEGPDAVLAARIRAAAGRPLVEHPDDSFDLHVDRSRGQVAENAAFL
                     ALLEADQAMLDKAVSAVTEGYRDPGAADLAMDSDYDLLGAEALASYCQAWDWLAGNPM
                     IGEPELIAVRESIVTRIDDFRRLVQEGPLLPMMLFARNNHVIKVLSSLGLCAMALNDR
                     REAARDMSHSLTGIQWVFVENQLTPDGGHAEGCHYLTYGSDTYLPFFFGLHRWADGKS
                     IPARVVGETQPDNPAAGQIIEVQDFATSPAVRAAYRLSLETVTPDGLCPSVDDSNGAA
                     QHGAILATMLGDDDFLWQWHRDAAGLDSAPLHALSLVAFNGLAPPLQPEAPLDFTRYG
                     AGISILRTSWEPDALYILLCGEHGPVRVNGLGHEHPDELAFIAWAHGKPLIIDPGYIN
                     YENHSLVFRPTDHNTILVDGKGSRFDSMSEMGLNVGNDAFLSCPGSQGDLTWTAVSTA
                     YEGVDFHRQILRVRSDYLVVHDVIRASQEHAYTLLLNGKGGGTTPDSSFQELDDGAIW
                     TNQGVAVRAMTVPLSGIVSVSSSLEEHNAPQWGQWLTHRRLAVNASMPPENAGFLTVL
                     AFAPSGGIMPELTAIPGSEGISVIRIDIPGEDPEWVVSAARSSEFSPEPGAPVSLEKG
                     FSMFSGTFSAEGAVLSGLPVLESAAGVADCGATGVRP"
     gene            6659..7627
                     /gene="pdxA"
                     /locus_tag="PKH54_03845"
     CDS             6659..7627
                     /gene="pdxA"
                     /locus_tag="PKH54_03845"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOC99051.1"
                     /translation="MKTVGITVGDPAGIGPEIVGRALATLTAAEPRAQTSFMLFGPAP
                     IIGQIVSGLPDAGRILPVATSPDVREVHAGRYTTASGSASLAALTAATDCLVDGSIGA
                     LATGPICKQAFADAGLPFPGQTEFVASRFGCTEWAMMLCGSRLKVALATTHVSIRELP
                     DAITADLVLRKLRVCDRFMKSHMGLKNPSIAVAGLNPHCGDGGMFGREDIEIIAPAIQ
                     TARNEGISATGPLAADTVFHRALSGEFDVVLAMFHDQGLGPLKLIHFSDAINITMGLE
                     RVRCACDHGPAFDLAGSGRADPSSMLAAIRFATRAAAGATPGRDRA"
     gene            7844..>8420
                     /locus_tag="PKH54_03850"
     CDS             7844..>8420
                     /locus_tag="PKH54_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC99052.1"
                     /translation="MNDRKVLIYEDDQTTLNQIQSVLTSLGLVPVIDTNPAAILEHAA
                     EHKPDLIILSADMKHGFSACLKIKKDKTLRRIPLFMISGKTAADVINKHQRLPTRADV
                     YLSTPLSTVMLTEILREQIPALPVQQAQPVTTGFEPPAEIDVNDIPDRTMVSARTIEA
                     AVVNFVEDEVRDLKSTVVKLQTEKQDLNGKLG"
BASE COUNT         1659 a         2703 c         2464 g         1594 t
ORIGIN      
        1 gccgacggag tgcacccgtc gcagcagcag ccctcgccgg aagccgcatc gcccttgaac
       61 atggccatcg cgatgtttgg gatctcgacc acaggcctga cggcacgcag gatgatttcc
      121 atctccagcg gattgctgat ggtttcaaga accagcaggt cggcgccggc atcagcaaga
      181 acggttgcct gtgatgaaaa cgcctgggtc agacgggcaa tcatcgcttc atcggaaaga
      241 tcgggaatca acccggaagg cccgatcgaa cctgcgacca gagcctgtcc accggccacc
      301 tccctggcaa tcctgacacc ggcgcggttg atttcatcca tcctctgggc aaggccatga
      361 ccggcaagcc ttaccgggtt tgcgccgaac gtgttcgtgg tcagaaccat cgcccccgcc
      421 gacaggtagt cggcgtgaac cttctcgacc atgtcagggc ggctgacgtt caactcgtca
      481 aaattgcgat tgatgaatat cccgcggctg taaagcagcg tacccatggc gccgtcgaat
      541 acgaccgggc ccttttcgag cagttgaaat atcctgtgcg ccatcgtcgt gtcaccggga
      601 ctggaaagcg gggaatgatt atacacgaaa atcgcggatc ggacggaacc ggtgaaccaa
      661 aggctcagtc cgcgtcagga tcgattacat cgccgggaaa gacgtcgatc ggcggctcac
      721 ccggcagcgg ggcaccggga tcgacctcgg tggcgtccga aagcggaagt tcggaaacct
      781 cgacgggatc gattacatcg gcgccgtcat cgggcgacca gggaacatcg gtaccggaaa
      841 gttcataacc catgtagtca ggctcgacga cgtcgggctg aatatagtct tccggtggaa
      901 ggcccatggt cggaacatcg acaaaagcac cggtgtcaaa cgtcgcgtca gtgccttcat
      961 ggacatccgg atccggaatc cttacatctt cttcgtacgg catcccgtca agctgcacca
     1021 catccgggcc ggcgtcctgg tcatcggata tctgctcgca gccggaagcc ccgatcatcg
     1081 caacggcgcc gccggcgaga acggaccact ttccggccgt caaggccagt tccataatcc
     1141 ttggatactt ccgggaatga cggtttgact tcattcgggc cacctgcctg cttgacgtgt
     1201 ccgattctac gtatcaccac acgtaaacgc aaatcaccta gcaaccggct cgaaagtgtg
     1261 aagactgttg gaaaacctta cgaaattgcc ggatccgtcc tgccagaccc ctatgactcc
     1321 gcagtcgcct tcgacccgga actcatatat gtcggctgtt ccgggcgccg caacctggtt
     1381 tgcaggttcg gcagcggccg tcgccggttc ggctgcaggt tcggcagcgg ccgtcgccgg
     1441 ttcggacgca ggttcggctg actccaccgt cggttctgtc gcagcagccc ggcccgttgt
     1501 ttcggcagcg ggtttcaccg gtttcgcggc ggccgccgcg ggctcgggcg acgtgaccac
     1561 cgtttcgggc gacgcgacct gggtggccgg ttccccggag ccggccgcct gaaccggtgc
     1621 cggatccgga accacggact ttacggaagt cacgccgacg atcctgacgg tggcctggcc
     1681 aatcttgcca agggtcgtgt tgatgcaggc gaacgttttt tccggaacgt tcctgtcaac
     1741 aatcatcgcg gccgtgtggg gctgatcttc ctcgacaagg aacaccggct gccacttgcc
     1801 gagaaccgaa accccggcct ttgaaccaag ggcatccctc atcctgacgt ctttctggta
     1861 tctgaatatc ttgaactcgc gaacgttgat tccctgggat tcccatctcg tgtacttcgc
     1921 aaatgtccga tcgggagcca tttccaggta cgaacgaacc gccggcgctt ccttcttctt
     1981 cgatttggcc gatgcggcgc ccttgaaata cctcgatgtc gcgaacacgc cctcttcgcg
     2041 gacaacctgg ttgaactcat accgcccgac cgtcttcata tccctgtcgc tgaccaggaa
     2101 gacggccgac tgcggttccg gacccgactg ggcaaggctg atttcgggaa ccagccagac
     2161 gccggcaatg aaggacgcga ccggcaggaa tgcgacgcct gccgcgactg ccgccgactt
     2221 caggatggaa cggttcttca tcggtttctc cacgaaactt cgacggaaat tgtaatacaa
     2281 gtgcgggggc aaggcccagt tcacgacttc ttgagggtat cgaccagttc agggacgagc
     2341 ttcaacgcat cgccgacgag acagtagtcg gcgattgaga aaatcggtgc ctcttcgtcc
     2401 ttgttgattg cgacgatgac cttcgagttc ttcatcccgg caagatgctg gatggcaccc
     2461 gacagcccga cagcaaaata aagcgagggg gcgacagcct tgccggtctg gccgacctga
     2521 aggtcgttcg gcgccactcc gttatcgacg acggccctgg tggcaccgat tgcagcgcca
     2581 aggtagtcgg cgaggggatt cagcgtgttc cagaacgatg ccttgtcgcc aaggccgcga
     2641 ccaccggaaa ccaccacgtc cgcctcggca agatccgggc gttcggaaac ggtcggttca
     2701 aacgaaacat gcctggcggc aggatcggca gccacatcga ctgcgagttc gaccaccggc
     2761 gaagaaccgc cggaaggctc ggctggactg aattctgtcc cgcggacgca tatcacgcaa
     2821 cgcggcgtct tgacccgaac cgtggcaatg acgttcccgg cccacatcgg gcgcctgaac
     2881 atcacatcgg agccgctgcc gtcgattccg accgcctcgc tgaccatgcc cgcgccaaga
     2941 cgcgcggcca cgcgtggaag catgtccctg gtccaggtcg tcgaaacggc caccagacag
     3001 tcggcgccaa ccgcctcgac catggaagcg gccgccccgg cccatggacg cgccagatac
     3061 gaagacaaac ccggagattc aaggacatgc actttcccgg ccccgaaacc ggcaagtgat
     3121 ggggctgcag ccctggcgcc aggccccatc acggcgatat cgaaggttcc ggaagttctt
     3181 ccggccaggt ccgccgcaaa ggtcacccct gacagggaag ccttgcgaac cctggaattc
     3241 tgaacctcga caaggacgag tatctttccc atctctgacc cccaaccagg ccgcggaagc
     3301 cctttcgccc cgcgtcccga cagcaattca tcaagccttc agaaccttcg cctcgttcct
     3361 gagaagatcg accagttccg cgacgcttcc aacgcgacgc ccaccggccc ttccggcagg
     3421 agcgctgtaa cccaccgtct cgaccttcat cgacatatcg acgccaagat cggccggggc
     3481 gtactcatcg acctgcttct tcttcgcctt caggacgtcc ggaagtttcg gcaggcgggg
     3541 ctcgttcagg cgcagatccg cggtgatgac cgcggggaac ttcacttcga cggtttccat
     3601 gcctccatcg acttccctcg acaccaccgc gccatcggaa ccaatcacga ccctggatgc
     3661 aaaacaggcc tggggaacgc cggcatactc ggcgacaagc tgcccgacct ggtttgaatc
     3721 gccatctacg gcctgctttc caaggatgaa aaggtcacag ccttccttcg caaagaccgc
     3781 cgcaagtatc cgggcggcca gatcggaatc gagctcaaaa tcgccggcct tgacgtgcac
     3841 gccacgatcc gcgcccattg ccagcgcggt tcgaagactc ttgaccgcct cctcgtcgcc
     3901 gatggtcacc gcgatcacct ctgcacccgt cttctccctg atggccaccg cttcctcgat
     3961 ggcgttctcg cagaacggat tcagtttgta ttccaggcct gccgtctcga tgccggtccc
     4021 atcgggaagt accttgatgc gcgcatccgg atcagtgacg cgctttgctg ttacaagtat
     4081 cttcacgttg cctcctgctg ttttgtgccc tgccggaaaa atcgattcta aactccagcg
     4141 gaagaatata taagatgttt ggttccgtcg tcatcaacaa ctggacaaaa gccgcaatgc
     4201 aagccgttca aatgatgttg aagcaccccg ccgccatttg cgtcatcctc gcttttctgt
     4261 cttccgcctg ccagtcgttc ggcgacgtga cgcaggacgc cctggatcaa gggacggtca
     4321 ttacggacgc ggcgcccgac tccgccgatc tgccggaatc cgattccacc ggcgaccttc
     4381 ccgactcggg ccaggcggac atcgccgcgg atggcgacgg ggccgatgtc ccgaccgatg
     4441 acggcttcct gccggacgag accggcggcg actcttcggt cgatgcggat gtaatcgatc
     4501 cggaggccga ctccgatggg gacggaattc cggatcttga tgaaatttcc gacggaaccg
     4561 acccgctgga tccctcggac gcaagggcgt ggcagccggg actcgggccg catccccggc
     4621 tgttcttcga cagcgacgaa atcccggcca tcgccgcgaa gctcgcggca accgagggac
     4681 cggacgcggt gctggcggcc cggataaggg cggcggcggg caggcccctt gtcgagcacc
     4741 cggacgattc ctttgacctt catgtcgatc gatcccgcgg ccaggttgcc gagaatgcgg
     4801 cattcctcgc cctgcttgaa gccgaccagg cgatgcttga caaggctgtt tcagccgtga
     4861 ccgaaggcta tcgcgatcct ggtgctgccg accttgcgat ggattcggac tacgaccttc
     4921 ttggtgccga ggcgcttgct tcatactgcc aggcatggga ctggcttgcc ggaaatccga
     4981 tgattggcga accggaactt atcgccgtca gggaatcaat cgtcacaaga atcgacgact
     5041 tcaggcggct tgtccaggaa ggccccctgc ttccaatgat gcttttcgcg cgcaacaatc
     5101 acgtcataaa agtcctttcg tcgctcggcc tgtgcgcgat ggcgttaaac gatcgccgcg
     5161 aggcggcccg ggacatgtct cactccctga ccggtatcca gtgggttttc gtcgagaacc
     5221 agctcacgcc cgacggcgga cacgcggaag gctgccatta cctgacctac ggctcggaca
     5281 cataccttcc gttcttcttc gggctccatc ggtgggcgga tggaaaatcg atcccggcca
     5341 gggtggtcgg cgagacccag cccgacaacc cggcggcagg acagatcatc gaagtgcagg
     5401 acttcgcgac gtccccggcc gtcagggcgg cctatcgcct gtcgctggaa acggttacgc
     5461 ccgacggact ctgcccatcg gtcgatgatt caaacggtgc cgcccagcac ggagccatcc
     5521 tggccacgat gcttggcgac gacgacttcc tgtggcagtg gcaccgcgac gcggccgggc
     5581 ttgactcggc gccgctgcat gccctttccc tggtcgcctt caatggtttg gcgccaccgt
     5641 tgcagccgga agctcccctt gacttcaccc ggtacggggc cggaatatca atcctgcgta
     5701 catcctggga gccggacgcg ctctacatcc tcctgtgtgg cgaacacggc ccggttcggg
     5761 tcaacggact cgggcacgaa cacccggatg aacttgcttt catcgcctgg gcacacggca
     5821 agccgctgat aatcgatccc gggtatatca actacgaaaa ccattccctg gtgttcaggc
     5881 cgaccgacca caacacgatc ctggtcgatg ggaagggttc ccggttcgac agcatgtcgg
     5941 agatgggcct gaacgtgggc aacgacgcgt tcctttcgtg ccccggttcc cagggtgact
     6001 tgacgtggac ggccgtctcc accgcctacg aaggcgtcga tttccatcgc cagattctgc
     6061 gggttcgaag cgactacctg gttgttcacg acgtgatacg ggcaagccag gaacacgcat
     6121 acacgctgct tctgaacggc aaagggggcg gcaccacgcc ggactcgtcc ttccaggagc
     6181 ttgacgacgg cgcgatctgg acgaaccagg gcgtggcggt tcgggccatg acggttccgc
     6241 tttccggaat cgtttcggtg tcatcaagcc tcgaggaaca caacgcccca cagtggggcc
     6301 agtggctgac gcaccgccgc cttgcagtga acgcgtcgat gccgcccgaa aacgccgggt
     6361 tcctgacggt gctcgcgttc gcgccatcgg gcggaatcat gccggagttg acggccatac
     6421 cgggttcgga aggcatttcc gtgatcagga tcgatattcc gggcgaggat cccgaatggg
     6481 tcgtctctgc ggccaggtca tcggaattca gtccggagcc aggagcgccg gtcagccttg
     6541 aaaagggctt cagcatgttt tccggaacct tctcggcaga gggtgcggtg ctttcgggcc
     6601 tgccagtgct tgaatccgct gccggcgtcg ccgattgcgg tgccacgggg gtgcggccat
     6661 gaaaacagtc ggaatcaccg tcggcgaccc tgccggcatc gggcccgaaa ttgtcggccg
     6721 cgcgctggcc acgctgaccg ccgccgaacc acgggcgcag acatcgttca tgcttttcgg
     6781 tcccgcgccg atcatcggcc agatagtctc cgggctgccc gacgctggcc ggattcttcc
     6841 ggtggccact tcgccggacg tgcgggaagt ccatgccggt cgctacacga ctgcttccgg
     6901 ctccgccagc ctggctgctc tgacggcggc gaccgactgc cttgtcgatg gttccatcgg
     6961 cgccctggca accggcccga tatgcaagca ggccttcgcg gacgccggac ttccatttcc
     7021 gggacagacg gaattcgttg cgtcccggtt cggatgcacc gaatgggcga tgatgctgtg
     7081 cggttcccgg ttgaaggtgg cactggcaac cacgcacgtt tcgatccggg aacttccgga
     7141 tgcaatcact gccgacctgg tgcttcgaaa gctcagggtt tgcgacaggt tcatgaaaag
     7201 tcatatgggc ctcaagaacc cttcgattgc ggttgccggc cttaatccgc actgcgggga
     7261 cgggggaatg ttcggacgcg aggatatcga aatcatcgcc ccggcgatcc agacggcccg
     7321 aaacgaaggc atttccgcaa ccggaccgct tgccgcggac acggtttttc accgcgccct
     7381 ttccggcgag ttcgatgtgg ttctggcgat gttccatgat cagggactcg gaccactcaa
     7441 gctgattcac ttttcggacg ccatcaacat caccatggga ctcgagcgag tcaggtgcgc
     7501 ctgcgaccac gggccggcct tcgaccttgc cggatccggc agggcagacc cgtcgagcat
     7561 gcttgccgcc atcagattcg ccactcgggc ggctgccggg gccacgccgg gtcgggatcg
     7621 ggcatgacaa ctccgcttat gccgcttttc aagaattatg cacatacacg cgcgcgcaac
     7681 gattgactat gaacgtcgcg gcgccttaaa atcctgccat tgattccgca agtgccatcc
     7741 cggcacctcc cggccggaat tgacaccggt tcggtgaccg gctaatgtgc cttcgcgacc
     7801 ggacggcctc gccaccatgc gggccggaca aggcaggaac tggatgaacg acagaaaggt
     7861 tctgatttac gaagacgatc agactaccct gaatcagatt caatcggtcc tgacatcgct
     7921 cgggctggtg ccggtaatcg acaccaatcc ggccgcaatc cttgaacacg cagccgagca
     7981 caagccggat ctgatcattc tttccgccga catgaagcat ggcttcagcg cgtgcctgaa
     8041 aatcaagaag gacaagacgc ttcgccggat tccgctgttc atgatttcgg gcaagacggc
     8101 cgcggacgtc atcaacaagc accagcggct gccgacaagg gccgacgtgt atctttccac
     8161 gcccctttcg accgtcatgc tgacggaaat ccttcgggaa cagattcccg cgcttccggt
     8221 gcagcaggct cagcctgtca cgacgggatt tgaaccacct gccgaaatcg acgtcaacga
     8281 cataccggat cgcacgatgg tgtccgcccg gacaatcgag gccgccgtcg tcaattttgt
     8341 cgaggacgag gtcagggacc tgaaatcaac ggtcgtcaaa ctgcaaacag aaaagcagga
     8401 tctcaacggc aagctcgggg
//
LOCUS       DAOPPA010000082         8403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9451_length_8403_cov_4.665189, whole genome shotgun sequence.
ACCESSION   DAOPPA010000082 DAOPPA010000000
VERSION     DAOPPA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_8403_cov_4.665189"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKH54_03855"
     CDS             complement(<1..888)
                     /locus_tag="PKH54_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOC99053.1"
                     /translation="MKRLTGDSRTDAAIILEAVLREHQRSTDLTNQAAGSYRDQRDFG
                     LLRELVGGVLRRMQALEAIVGPHVGRGLENTRPLLKMILLGHAYQAVFLDRIPAHARV
                     SGAVNAARRLDGEKGAGFVNAVTRSVERLADGNPGRLLDGLTPWQLLSIPPVFMEHIR
                     LAHGGVPGADDLARLASPSPISVRIRHRGESRERALAALAATGTVATPGEFAPDCMIL
                     DSGAILATDAVPSLVIPQDQASQLVAMALGPLDGANVLDMCCGVGIKTSQILDMSDQA
                     TVTAVDTDSAKLDRNKELCR"
     gene            complement(885..1871)
                     /locus_tag="PKH54_03860"
     CDS             complement(885..1871)
                     /locus_tag="PKH54_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOC99054.1"
                     /translation="MTEETKKAFKARLRAVIAWTGTVGLLAFLYFTTDRDAVAAAIGR
                     ADPVLFIATAFIAVAATYMTDVFTVKFLLGRVGIKVGFGEFARIKGASYLLNIINYNL
                     ALVMMAAVVKKRSSKGWGAAGSPFVLLNFIDLSVFSIIVQAAIWTGRSPFDRAPTMIL
                     AILTLGGVLAAPALCLISRIRNAPGWFGRVVNHDIMAAFRYIGPWSLLTMLAFRSILI
                     LEYGAMNLFFMRSFGVSVPPLTLLFFMAITSFIAMVPISVSGIGSTQVAMRGLYGPFV
                     PDGVAPTAASKIAVVDAFSTAGIFGVLLIRVVLGIICMRDVSRYISEDGEKS"
     gene            1996..4302
                     /locus_tag="PKH54_03865"
     CDS             1996..4302
                     /locus_tag="PKH54_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99055.1"
                     /translation="MNSIASDRESGIGLDVVFLFFYPSRGDTFRFPALSACCSGEVIA
                     RGLRSRVVEVQLDIDSPAETDLALKLLSDFLRAQSPKVVVCEEVPFPGVFDVIRKACD
                     ASIVYPFEADPASAHLIDFKVFNFQSNPAPLVELVTRLANGREPDGIPNVSVHGRPPA
                     TLDAECGRPPLFLADPDYDFVSIPACARQASRRLSVYVNPGCPWSADVADNPHYSGVT
                     FAIDGVARRGCSFCFQDGRYSGLDAEATVTRILLELVRWLGLHPECREVVLWDESPWR
                     FLGLLVERLASSGMGPMAVCFHARCDHLVRQVATITDACLRARQFADSGISLVLTLVG
                     FENYSAPELERMNKGYGPPILSQAAAVCRSIADQYPDVFEYDRYRASSFILFTPWTLV
                     SDLRANIDGFRRDGIEEFSTGMGLSRLRLYPNLPITALADSDGLARERATGDAENLAG
                     MFGYSFDIPWRFRDGRVEQVFRLYRALYPLVERRWQVDLLEWCVNAVVSGDSRLFDPP
                     SAASVFLRLNAALAALNSRGRGTARKPESVVAAGATDPRAESMRTGTLELILTGRGVP
                     DGRNLPDTALDPSPARLAVKISRAADAVSRLHVLGREPTLSPIFLRAVYLARQAGVPG
                     ASVRTDGLVFSQPAALARAIKAGLTDIEIRVFGTSDEDWRRATGGRGGIDAFLAAASL
                     VASSSAAIGGRALVQIGTVNPDSIPQVVSMLDSVGLRQVSFEAPVSCLPVSGLESFVR
                     EIERLVQSSSPVPASDPEVGIDNGNIPD"
     gene            complement(4258..5940)
                     /locus_tag="PKH54_03870"
     CDS             complement(4258..5940)
                     /locus_tag="PKH54_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99056.1"
                     /translation="MQDTVVTIETGLGCNNRCAHCPQPAIRAAGPVTQPSFQEIRQKI
                     DTARAQGFTQISFSGGEPTIRRDIAELVNYARTTGFQKVSITTNGRMFSYPKFADLML
                     KAGLTGVSVSLHGPTAEIHESLTMVPGSFEQALAGIRYLKERTSATGAEFDITTITVL
                     VPGNIDLLRETLLLAQEHGATVHIVQPFILSKENLARGARFLLGIDRIVAGIRTALEG
                     GLPNGRVKPYNIPPCLLSDIGPEIEVQSYHLRTVREFDPVDGTSAVGTVAGQFFRYSQ
                     CSQCDAICPGLRIEHVSQTAMVKMILADIMSSRPFVLPKSSSLSSTDLLEAPALDRLL
                     GELARAGATSPVLFWGGIGLCPQEDFMEVCRRRNVSQVILVARPPQLRPPDARVSIPG
                     NIAEIRELLKRFRTGQVPVPALFIVINSVYSEQCDFNRKELLDLVDALVEAGGRRLLL
                     SAPEGMDPLLPAHTDELKDRVIADLPALLAELAERGIEPCLSRTVGVDLDGHDNLLEA
                     RVAEFIRPVNLNDDFLHHRFAAPENGWIMWSYPIWIHRSVRDVPVVDADLGV"
     gene            6082..7827
                     /locus_tag="PKH54_03875"
     CDS             6082..7827
                     /locus_tag="PKH54_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC99057.1"
                     /translation="MPRIINYERPLFRGHAPFSVYDVPIALAVFALIWAFLTVGRDMT
                     APVLPDSGELDLAFIRLPYYALRSVARMFLAYFFSLLFTFVFGSLAAHNRTAEKVIIP
                     TIDILQSVPILGFLAVTVNGFMSLFPGSMLGIELASIFAIFTSQVWNIVYSFFQSVRA
                     IPRDLTDSAAVFKLTPIQRFFRLEVPFSMIGLVWNSMVSFGGGWFFLAASEAITVLGK
                     DIRLPGLGSYLATAVEKGDFPAIGAAIAVMLLIVVLLDLAFFRPLLAWAQKFRTGDVF
                     ETEYESAVLTAIQRSTLVETFVEKVSRPFWSFLIDRLPARLAASRIGSLAGRRRRGLS
                     ITGIILLVLGGGMVFFMVGDGVVFLFQSMTLGELGVIGLSGLATMARVLATVGVASLV
                     WVPIGTYIGMRPGLARRIRPVIQIGAAFPANLIYPLVFLLFVRAGVDLGIGAVVLMFI
                     ATQWYILFNVIVGTLTVPEDLKAAADVMRLRGFMRFRTLVLPIVFPFWVTGALTAAGG
                     AWNASIVAEVASFGDHSMKAFGLGAYIAQATVQGDWPGIVVSIVVMCLLVVLMNRFLW
                     RRLYRYAEEHFTLEG"
     gene            7959..>8403
                     /locus_tag="PKH54_03880"
     CDS             7959..>8403
                     /locus_tag="PKH54_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC99058.1"
                     /translation="MWFGGTSDTPRVTVLEGVDFTIREGEFVALLGPSGSGKTTFMRI
                     LAGLTEPSAGEVLYHGQRLVGVNPGVSVVFQSFALFPWMSVLENVAMGLRAAGMNLKD
                     AMEKATAAIDMIGLDGFEDALPRELSGGMKQRVGFARALAVEPEVL"
BASE COUNT         1471 a         2555 c         2585 g         1792 t
ORIGIN      
        1 ccggcacagt tccttgttcc tgtccagctt cgccgagtcc gtatcgaccg ccgtcaccgt
       61 cgcctgatca gacatgtcca gtatctgcga tgtcttgatg ccgaccccgc agcacatgtc
      121 cagcacgttt gccccgtcaa ggggaccgag ggccatcgcc acaagctggg acgcctgatc
      181 ctggggaatc accagcgatg gaaccgcgtc ggtcgcaagg atggcgccgg aatcaagaat
      241 catgcagtcc ggcgcgaatt cccccggagt agcgacggtt cccgttgcag ccagggccgc
      301 aagtgcgcgt tcccttgatt ccccgcggtg tcgaatcctg acggaaatcg gtgacggact
      361 ggccagcctg gcaaggtcgt cggcgcctgg aacgcccccg tgggccagcc ggatgtgttc
      421 catgaaaacc ggcgggattg acagaagttg ccagggcgtc aatccatcaa gcagcctgcc
      481 gggattcccg tcagcaagcc tttccaccga cctggtcacc gcgttgacaa accccgcgcc
      541 cttttccccg tcaagcctgc gcgcggcgtt gaccgcgccc gagaccctgg catgtgccgg
      601 gattcgatca aggaacaccg cctggtacgc gtgtccaaga aggatcattt tcagcagggg
      661 ccttgtattt tccaatccgc ggccgacgtg cgggccgacg attgcctcca gggcctgcat
      721 gcgccgcagg actccgccga ccagttcgcg cagcagcccg aagtcacgct ggtcacgata
      781 agacccggcg gcctggttgg tcagatccgt gctgcgctgg tgttcgcgaa gcaccgcctc
      841 caggatgatg gcggcgtcgg ttctggaatc cccggtcagc cgcttcaaga tttttccccg
      901 tcttcggaaa tgtatcgaga tacgtcccgc atgcagatga ttccaagcac gaccctgata
      961 agcaggacac cgaagattcc ggctgtcgag aaagcatcaa ccaccgcgat cttcgatgcg
     1021 gccgtggggg cgacaccgtc cggaacgaag gggccgtaaa gaccccgcat cgccacctgg
     1081 gtcgagccga tgccggatac cgaaattggc accatcgcga tgaacgaagt gattgccatg
     1141 aagaacagca gcgtcagcgg tggaaccgaa accccgaagc ttctcatgaa gaagaggttc
     1201 atcgcgccgt attcaagaat caggatcgat cggaacgcga gcatcgtcag aagcgaccac
     1261 ggcccgatat acctgaacgc cgccatgatg tcgtggttga cgacgcgtcc gaaccagccc
     1321 ggggcgttgc gtatccgcga aataaggcac agcgcgggcg cggcgagcac tccgccaaga
     1381 gtcagtatcg caagaatcat ggtcggcgcg cgatcaaatg gcgagcgccc ggtccagatt
     1441 gccgcctgga ctatgatgct gaatacgctc aggtcaatga agttcagcag gacgaacggg
     1501 cttccggcgg cgccccagcc tttggaactc cgcttcttca cgacggcggc catcatgaca
     1561 agggcaaggt tgtagttgat gatgttaagc aggtacgacg cgcccttgat cctggcgaat
     1621 tccccgaatc cgaccttgat cccgacacgg cccagaagaa acttcaccgt gaaaacatcg
     1681 gtcatgtagg tcgcggccac cgcgatgaat gccgtcgcga tgaaaagcac cggatcggca
     1741 cgcccgatcg ctgcggcgac cgcatccctg tcggtcgtga aatacaggaa ggcaagcagg
     1801 ccgactgtgc ccgtccatgc gattaccgcc cggagccttg ccttgaaggc cttttttgtt
     1861 tcttccgtca cgggcggtat tgtaggccag tccccgaatg gccgcgacaa gaaatgcgac
     1921 gtcgggccgt gtccgtacgg acgtgctaga atcggccgca cgatcggtcg ttttgtccgg
     1981 tctcacgggg tgtccatgaa ctccattgct tcagacaggg aatcaggaat cggacttgac
     2041 gtggtttttc tgttctttta cccgtcccgc ggcgacacgt tcaggttccc ggcactttcc
     2101 gcctgctgca gtggcgaggt tattgcccgc ggattgcggt cccgggtggt tgaagtccag
     2161 cttgacatcg acagtccggc cgagactgat ctggcgctta agctgctttc ggatttcctt
     2221 cgcgcgcagt cgccgaaggt cgtggtctgt gaagaagttc ccttcccggg cgtctttgat
     2281 gtcatcagga aggcgtgcga tgcatcaatc gtgtatccgt ttgaagcgga tccagcgtcg
     2341 gcccacctga ttgatttcaa agtcttcaac ttccagtcaa acccggcgcc gcttgtcgag
     2401 ctggtgacgc ggcttgcgaa cggtcgcgaa ccggacggga ttccgaatgt ttccgtccac
     2461 ggacgcccgc cggcaacgct tgatgccgaa tgtggcagac cgccactgtt tctggccgac
     2521 cccgactatg atttcgtgtc gattcccgcc tgcgcccgcc aggcttcaag gcgtctttcc
     2581 gtctatgtga atcccgggtg tccgtggtcc gccgacgtcg cggacaatcc gcactattcc
     2641 ggggtaacgt tcgcgattga cggcgtcgcc aggcggggct gttcgttctg tttccaggac
     2701 ggtcgatatt ccgggctcga tgccgaagcg accgtcaccc gtatcctcct ggaacttgtc
     2761 cggtggcttg gccttcatcc cgaatgccgg gaagtggtcc tgtgggacga gtcgccctgg
     2821 agattccttg gattgctggt tgagcgcctg gcgtcgtccg gcatgggccc catggcggtc
     2881 tgcttccacg cacgatgcga tcatcttgtc aggcaggtgg caaccatcac ggacgcgtgc
     2941 ctgcgcgcca ggcagttcgc ggattccgga atatcgctgg tgctgaccct ggtcgggttc
     3001 gaaaactatt ccgcgccgga actggagcgc atgaacaagg gatatgggcc gccgatcctg
     3061 tcgcaggcgg cggccgtctg caggagcatt gccgatcagt acccggacgt ctttgaatac
     3121 gatcggtatc gggcgtccag cttcatcctt ttcacgccct ggaccttggt gtccgacctt
     3181 cgggccaaca tcgatggatt caggcgcgac ggcatcgagg aattttcgac cggcatggga
     3241 ctttcccggc tgcgcctcta tccgaatctt ccgataacgg cccttgccga ttcggacggg
     3301 cttgccaggg aacgggcaac cggcgacgcc gagaaccttg ccgggatgtt cggttactca
     3361 ttcgacatcc cctggcggtt ccgggacggt cgcgtcgagc aggtcttccg gctatatcgt
     3421 gctctgtatc cactggttga acggcgctgg caggtcgatc tgcttgaatg gtgcgttaac
     3481 gcggtcgttt cgggcgattc caggctgttt gatccgccat ctgccgcttc ggtgttcctg
     3541 cggctcaacg cggccctggc tgcactgaac tcaagaggcc gtggcaccgc gcgaaagccg
     3601 gaatccgtcg tggccgctgg cgccaccgat ccgcgcgcgg aatcgatgcg aacaggcacg
     3661 ctggaactga tcctgaccgg gcgcggggtt cccgatggcc gcaaccttcc cgacacggca
     3721 cttgatccct cgccggccag gcttgcggtg aagatttcca gggctgccga tgcagtctca
     3781 aggctgcatg ttcttggtcg cgaaccgacc ctttccccga tcttcctgcg ggcagtctat
     3841 cttgcgcgcc aggcgggggt tccgggcgct tcggtgcgca cggacggcct ggtgttttcc
     3901 cagccggcgg cgctggcccg cgcgataaag gccgggctga ccgatatcga aatcagggtc
     3961 ttcggaacat ccgacgaaga ctggagaagg gcgaccggcg gccgtggcgg catcgatgcg
     4021 ttcctggccg ccgcgtccct tgtcgcgtct tcatccgcgg caatcggagg gcgcgctctc
     4081 gtgcagatag gaaccgtgaa tccggattcg atcccgcagg ttgtttccat gcttgattcg
     4141 gtcggtcttc gccaggtctc cttcgaggcg ccggtgtcct gcctgccggt ttcggggctg
     4201 gaaagtttcg tgcgtgaaat tgagcggctg gttcagtcct cgagccccgt tcccgcctca
     4261 gaccccgagg tcggcatcga caacgggaac atcccggact gacctgtgta tccagatcgg
     4321 gtatgaccac atgatccagc cgttttccgg cgccgcgaag cggtggtgaa ggaaatcgtc
     4381 gttaaggttc accggcctga tgaattcggc aacccttgct tccagaaggt tgtcgtgccc
     4441 gtccagatca acgccgacag tgcgcgaaag acaaggttcg atgccgcgct cggcaagttc
     4501 cgcgagcagg gccggaagat cggcaatgac acggtccttc agttcatccg tgtgtgccgg
     4561 cagaagggga tccattcctt ccggcgccga cagcagcagg cgccggcctc cggcctcgac
     4621 cagggcatcc accagatcca gcagttcctt tcgattgaag tcgcactgtt cgctatagac
     4681 gctgttgata acgatgaaaa gcgccggaac cggaacctgc cccgtcctga agcgcttcag
     4741 gagttccctg atttcagcga tgttgcctgg aatcgaaacg cgggcgtcag gcggcctcaa
     4801 ctgaggcggc ctggccacca ggatgacctg gctgacgttg cgcctgcggc agacctccat
     4861 gaagtcttcc tgcggacaaa gccctatgcc tccccagaac aggaccgggg atgtcgcccc
     4921 ggcgcgtgcc agttcgccaa gaagcctgtc cagggccggt gcctccagaa ggtcggtcga
     4981 tgaaagcgac gacgacttcg gcagcacgaa aggtcgcgac gacatgatgt cggccaggat
     5041 catcttgacc atggccgtct gggatacatg ttcgatcctg agtccggggc agattgcgtc
     5101 gcactgggaa cattgtgaat accggaagaa ctggcccgcg acggttccga cggccgatgt
     5161 gccgtcaacc gggtcgaact cgcgaaccgt ccgaaggtgg taactctgga cttcaatttc
     5221 gggcccgatg tcggacagca ggcagggcgg tatgttgtac ggcttgaccc ggccgttcgg
     5281 aagcccgccc tcaagcgcgg ttcgaatgcc ggcgacaatc ctgtcgatac ccagcaggaa
     5341 cctggctccc cttgcaaggt tctccttcga aaggatgaac ggctgcacga tgtgcacggt
     5401 cgccccgtgt tcctgcgcaa gcagcagggt ctcacgcagc aggtcgatat ttccggggac
     5461 gagcacggta atggtcgtga tgtcgaattc ggccccggtt gccgacgtcc tttccttcag
     5521 gtaacggatt ccggccagcg cctgctcgaa cgacccggga accatcgtca acgattcgtg
     5581 gatttcagcg gtcggcccgt gaagagacac gctgaccccg gtcaggccgg ccttcagcat
     5641 caggtcggca aacttcgggt acgagaacat cctgccgttt gtcgtgatgc tgaccttctg
     5701 aaagccggtt gtccttgcgt agttgaccag ttcggcgatg tcccgccgaa tcgtcggctc
     5761 gccgccggaa aaagagatct gggtgaatcc ctgggccctt gccgtgtcga ttttctgcct
     5821 gatttcctgg aatgaaggct gggtcaccgg tccggccgcc ctgatggccg gctgggggca
     5881 gtgcgcgcac cggttgttgc atccgagtcc ggtttcgatg gtgacgaccg tgtcctgcat
     5941 gtgtctctcc agcaccaccg cggatgatat cagttatgcg tcggggccgc atcaatgcgg
     6001 tttgacactt cgggtcgggg ggcggaaaat acccgcgatt ccagggtacc aaggggtcag
     6061 cctgaacgaa gatggacaag gatgccgcgg attatcaatt acgaacggcc gctctttcgt
     6121 ggacacgcgc cgttctccgt ctatgatgtc ccgatcgccc tggcggtctt cgcgctgatc
     6181 tgggccttcc ttaccgtcgg cagggacatg acggcccccg ttcttccaga ttcgggcgaa
     6241 ctcgatctgg ctttcatcag gctgccatat tacgcgctgc gttcggtcgc gaggatgttc
     6301 cttgcgtatt tcttttccct tcttttcacg ttcgttttcg gttcccttgc cgcgcacaat
     6361 cgaaccgccg aaaaggtcat cataccgaca atcgacatcc tccagtcggt tccaatcctt
     6421 ggcttcctgg ccgtgaccgt caacgggttc atgtccctgt ttcccggttc catgctgggc
     6481 atcgagctgg catcgatatt cgccatcttc acctcccagg tgtggaacat cgtctatagc
     6541 ttcttccagt ccgttcgggc gattccaagg gatctgacgg acagcgccgc cgtgttcaag
     6601 ctgaccccca tccagagatt tttcaggctg gaagttccgt tttccatgat cggcctggtc
     6661 tggaacagca tggtgtcgtt cggtggcggc tggttcttcc ttgcggccag cgaggccatc
     6721 accgtccttg gcaaggatat caggctcccg gggcttggtt cgtacctggc aaccgccgtc
     6781 gaaaagggcg acttcccggc catcggcgcc gcgattgcag tcatgcttct gattgttgtc
     6841 ctgcttgacc tggcattctt ccggccgctg ctggcatggg cccagaagtt ccggaccggc
     6901 gacgtgttcg aaaccgagta cgagtcggcg gtcctgaccg ccatccagcg ttcgacgctg
     6961 gtcgaaacgt tcgtcgaaaa ggtcagccgg ccgttctggt ccttcctcat cgacaggttg
     7021 ccagcgcgtc ttgccgcctc gcgaatcggt tcgttggccg gtcgccggcg tcgtggactt
     7081 tcgataaccg ggatcatcct tcttgtgctt ggcggaggca tggtcttctt catggttggc
     7141 gacggcgtcg tcttcctgtt ccagagcatg accctggggg aacttggcgt aatcgggctt
     7201 tccggccttg caacgatggc gcgggtgctt gcgaccgtcg gggtcgcgtc gcttgtctgg
     7261 gttccgatcg gaacctatat cggcatgagg ccggggcttg ccaggcgcat ccgcccggtc
     7321 atccagatcg gcgcggcctt cccggccaat ctcatctatc cgctggtatt ccttctgttc
     7381 gtccgggccg gcgtcgatct tggaatcggg gcggtcgtgc tgatgttcat agcgacccag
     7441 tggtatatcc tttttaacgt cattgtcggc acgctgaccg ttccggaaga cttgaaggcg
     7501 gccgcggacg tcatgcgtct gcgcggtttc atgcgcttca ggacccttgt cctgccgatc
     7561 gtctttccat tctgggtcac cggggcgttg acggccgccg gcggcgcgtg gaacgcgtcc
     7621 attgtcgccg aggtggcatc gttcggggat cactcgatga aggcgttcgg gcttggcgcc
     7681 tatatcgcgc aggcgacggt gcagggcgac tggcccggaa tcgtggtttc aatcgtcgtc
     7741 atgtgtctgc tggtcgtgct tatgaaccgc ttcctctggc ggcggcttta tcgatacgcc
     7801 gaggagcatt tcacacttga aggttaacgg cccggggccg tccggaactt gggggaacgg
     7861 caatgtcgga acttgatcgc gcaaaaagaa agattgatgc cctttcgatc caggatcgcc
     7921 aggatgttcc cgccatactc cagacgaaag gcctgagcat gtggttcggc ggcacgtcgg
     7981 atacgccgcg cgtgacagtt cttgaaggcg tcgatttcac tatccgcgaa ggcgagttcg
     8041 ttgccctgct cggtccgtcc gggtctggaa agaccacgtt catgcgaatc cttgccggtc
     8101 ttaccgaacc ttcggccggt gaggtgctct atcacggcca gcgccttgtc ggcgttaatc
     8161 cgggcgtttc ggtcgtattc cagtcctttg ccctgttccc gtggatgagc gtgctggaga
     8221 acgtcgcgat ggggctgcga gccgcgggca tgaaccttaa ggacgccatg gaaaaggcga
     8281 cggcggcgat cgacatgatc ggtcttgacg gcttcgagga tgcgcttccg cgcgagcttt
     8341 ccggcggcat gaagcagcgg gtcggattcg ccagggcact ggcggtcgaa ccggaggtcc
     8401 ttt
//
LOCUS       DAOPPA010000083         8296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9624_length_8296_cov_4.209077, whole genome shotgun sequence.
ACCESSION   DAOPPA010000083 DAOPPA010000000
VERSION     DAOPPA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_8296_cov_4.209077"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKH54_03885"
     CDS             complement(<1..699)
                     /locus_tag="PKH54_03885"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="s-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOC99059.1"
                     /translation="MDIRPVFRDGLKLFMLDQRLLPHREVFLEYHDAAGVAGGISDMV
                     VRGAPAIGIAAAYGIALEAFKAPDSELFQDIGRAAAILMASRPTAVNLAWAVNRMVRT
                     AGEAGQMRPQAIRARLLAEADAILAEDIEMNRRIGSFGAALLPDRCRVMTHCNAGALA
                     TGGFGTALGVIRAARDAGKDISVISCETRPYLQGARLTCWELVRDGFDVTLITDNSAA
                     WQMASGNVDCVIVGA"
     gene            complement(719..2689)
                     /locus_tag="PKH54_03890"
     CDS             complement(719..2689)
                     /locus_tag="PKH54_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOC99060.1"
                     /translation="MFGSPSTKQLRLFLFSIPALLAISGLLPGCTPDRPASRAAGGRT
                     AVVSPEQEAVGAEPLESGLMQMWNALNSSRTVVRARQQDSIAGMKEWSEAVTSAMDAI
                     EWQPVFTDDTGTTDLFDKLLAALEALPTHGLELESSGLPELKTALEAYAPGVDAESEI
                     LAGMSDLSGWEKLSGLVDRREKPTAEEIASILEDPERISGDDLAKLVEGVGKLAAIRK
                     EYAAPRGQVESLGILAYMKYAMNMRFRVVADPFMAQRNPATAHRQFTQKLIESLKSFI
                     QDPDTTLSKLPPSHPYYAKTMAGLAAYLEMAAKGDFPEVRIPRKMAPGTKSSLIPEIK
                     NRLAREGYFSGDSNEKFDDDLKQAVIRYQETHGFNPTGVLEPSHGKSMNIPVSKRIEQ
                     IRLSLQRWRESEIRDGGELYVRVNIPQFMMEVWEGSERKFRNRVVVGNNGWDTDPGAK
                     VEGRINRTKIFSAQIQDIVLNPRWNVPMRIRKVELEMDLLSQPDYLLKNNYVVEMLPD
                     GREIVYQKSGDGNALGRIKFNFPNQYGIFMHDTNLKRFFGKEIRAFSHGCVRLDDPVP
                     IAKYLISVASETSPDEVDRELAKAEPDPRSVKLSRPVPIYIEYNSAGPDDDGKMMFFS
                     DIYNYDRDYFAGKIPYSTDELELLRRKITQPD"
     gene            2883..3734
                     /gene="nadC"
                     /locus_tag="PKH54_03895"
     CDS             2883..3734
                     /gene="nadC"
                     /locus_tag="PKH54_03895"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOC99061.1"
                     /translation="MTGNLFGGNPLVLELVRLAIAEDIGTGDVTTGLVIRPGAQASGS
                     ILARQSGIVAGLPVVEMVFSEIDPAIRFEAKVADGQAVEPDQVLAAVSGPAASLLRAE
                     RTVLNFLMHLSGVATHASRFARAISGTGIRLLDTRKTTPGNRILEKYAVAVGGGMNHR
                     MNLAGGVLVKNNHIAVCGGVEQAVGLARAGAPVTLKIEIEVRSVSEAVRAARAGADML
                     LLDHMGPSEAAEVRQAVGPAVLLEVSGNMTPEKAVAYGGTGIDFVSAGSVVYGAVWLD
                     ISMYLEL"
     gene            3749..4498
                     /locus_tag="PKH54_03900"
     CDS             3749..4498
                     /locus_tag="PKH54_03900"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOC99062.1"
                     /translation="MDAAKVCEILRSGGVDARFAAVVESTQDECIRAGRGGAPSGSLV
                     LASSQVAGRGRMGRRWLSPAGGLYASIMLRPALRQELWSALTLHAGLAVVSVVRAAGV
                     ADARLKWPNDCLAGGRKLCGILAESFPGDGIVVIGLGMNIESAPDVGSVECLHHGLPS
                     AFLDGLVDVDHDVFCADLLFRMRSALLRADFGLPLDTRAISEMLWDGGTINACCDGSE
                     FSGRATGVDPLGRLIVLLPDGSVRHVSTGEV"
     gene            4500..5309
                     /locus_tag="PKH54_03905"
     CDS             4500..5309
                     /locus_tag="PKH54_03905"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOC99063.1"
                     /translation="MLVAIDTGNTNTVFGIYQGSDLKASFRVSSRRDATLDEWLSSID
                     GLLSFKGIDRRMVDSACIASVVPSVGSMMEDVVRSLFSVEPLVVGPGTRTGISICYES
                     PSDVGPDRIVNAVAAHARYGMNTIVIDLGTATTFDAVTASGQYLGGVIVPGIAMGLDS
                     LSARTARLPRVGFARTGHVIGRNTVASIQSGAYWGLVAMLEGLTRRMMGEFEGPVRVV
                     ATGGLASLVCGDCPFIDEVDGDLTLEGLRIIWDKNRGPARDFGTTADRGQH"
     gene            5306..>8296
                     /locus_tag="PKH54_03910"
     CDS             5306..>8296
                     /locus_tag="PKH54_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HOC99064.1"
                     /translation="MNKLFCKRGLAGRMIRFAATLAGTGLVIWILLFSGIYPRQTVFS
                     LAESQPASALEYRKDDLARLRLLTRCIKYIRSEYVVPEKVKPLPMLAGALKAAEGLVP
                     DLMVSFNRDDVETATSVEVRIGEVTKVFPLEKTSDLYQMNWKLVDMFEFISAHLPADV
                     KADEVEYAAINGMLQPLDEHSVYLPPEAYQQMQLDTQGRFGGLGIVISSRNGYVTIMS
                     VMEDTPAWRAALKSGDQIVEINEESTMNMPLTDAVSRLRGEPGTKVSMVVDRKGWSEP
                     RTITIERAEIKVQSVKTQSLGGGVGYARLVHFQEDTAAELSRQLEELRRRGSLRGLVL
                     DMRENPGGLLDQAVAVGDLFLRSGTLVVTEGEGNRLRQAFDADSGEAFENLPLVVLVD
                     GGSASAAEIVAGALKYNDRAMLVGSTTFGKGTVQVMHEVGDGALKLTVAQYLIPGDLS
                     IQGVGVVPDIELVPTSIGRKNILMGLTDYKRFMDPSRRLDAFGKVASDIPVHVIPFLM
                     KSEEEQEEEDDVLDELPIEDDSFKRDEYIDLAASIVASTVAFADVSRKSQLKNGETAA
                     LDWKNAQDAKISASLVEKGVDWAAGPVVSGASVSTGFSAGSAPLAAGSMAVMNMMVTN
                     GGKEPLFRVRASLKSDNLSLDGREFIFGRIGPNETVSRQVQFKVPRETWQRRDQVEIS
                     LYQNAVEAIPGGTRVEYVEFAPLPRPRFGFSAMVLDRQGNGDGILNPGEKADVVFPIA
                     NVGSGDAPRVMASIRNRSGESLYLRTGRETIREGIKAGASGLAKFAVERRAADSPAKV
                     TGGQGATGGAKVEFSIFDLALREYLAEEIELPSASEPGAAFTAKPGTIRTVGPGTRIL
                     AAASADSMVLAEVPEGTTLRSTGFFPGFFKVDLANGLTGFVTSTDVMEPGTASGSDLL
                     PEPSGTAHLAPDISVEFVRGEGSSPVLKVRGKVGFSVGSGDVRRKILIFRESDKVYFW
                     AGKGGSDSREVPLDTTVKLRPGRNA"
BASE COUNT         1567 a         2345 c         2620 g         1764 t
ORIGIN      
        1 ggcaccgaca atcacgcagt caacattgcc gcttgccatc tgccacgccg ccgaattatc
       61 ggtgatcagg gtcacgtcga atccgtccct gaccagttcc cagcaagtca ggcgggcacc
      121 ctggaggtac ggcctggtct cgcacgatat caccgaaatg tcctttccgg cgtcgcgggc
      181 ggcccggata accccgaggg ccgtgccgaa gccaccggtt gcaagcgccc cggcattgca
      241 gtgcgtcatg accctgcagc gatctggaag aagcgcggcg ccgaacgaac cgatcctgcg
      301 gttcatctcg atatcttcgg caaggatcgc gtccgcctcc gccagcagcc ttgcccttat
      361 ggcctgcggg cgcatctgtc ccgcctcccc ggccgtcctg accatccggt tcaccgccca
      421 cgccaggttg accgcggtcg gtcttgatgc catcagaatc gcggccgccc ggccgatatc
      481 ctggaacagc tcggagtcgg gcgccttgaa cgcctcgaga gcaattccgt atgccgcggc
      541 gattccaatt gccggcgcgc cgcgaacgac catgtcgctg ataccgccgg ccacgccggc
      601 cgcgtcgtga tactcaagaa agacttcacg gtgcggaaga agacgttgat cgagcatgaa
      661 cagcttcagc ccgtcgcgga aaacagggcg gatatccatc gttggcctcg caagtgactc
      721 aatcgggctg ggtaatcttt cgccgcagca attcaagctc gtcggtcgag tacggaatct
      781 tgccggcgaa gtagtcgcgg tcgtagttgt agatgtccga gaagaacatc atctttccgt
      841 cgtcgtcggg gccggccgaa ttgtattcga tataaatcgg aaccggccgg ctcaacttga
      901 ctgacctggg atcgggctcc gcctttgcaa gctcgcgatc cacttcgtcg gggcttgtct
      961 cggaggcgac cgaaataagg tacttggcaa tcggaaccgg gtcgtccaac ctgacgcagc
     1021 catgggaaaa ggcccggatt tccttgccga agaacctttt caggttagtg tcgtgcatga
     1081 agatgccgta ctggttgggg aagttgaact tgatccttcc gagcgcgttc ccgtcgccgg
     1141 atttctgata aacgatttcg cgcccgtcag gcagcatctc gaccacgtag ttgtttttca
     1201 gcaggtagtc gggctgggaa agaagatcca tttccagttc gacctttctg attcgcatcg
     1261 gaacattcca gcgcggattc aggacgatgt cctggatctg ggcagagaag atcttcgtgc
     1321 ggttaatcct gccttccacc ttggcgccgg gatcggtatc ccaaccgttg tttccgacaa
     1381 cgacccggtt gcggaacttc ctttcgcttc cttcccagac ttccatcata aactgcggga
     1441 tgttgacccg cacatacagt tccccgccgt cgcgaatctc gctctcgcgc catcgctgaa
     1501 gggaaagccg gatctgctcg attcgcttcg agacaggtat gttcatcgat ttgccgtggg
     1561 atggctcgag tacgccagtc ggattgaagc cgtgggtttc ctggtacctg atcacggcct
     1621 gtttcagatc atcgtcaaac ttctcgttcg aatcccctga gaaataccct tcgcgggcga
     1681 gacggttctt gatttcgggg atcagcgagc tcttcgtgcc aggcgccatc ttccgcggga
     1741 tcctgacctc cgggaaatct cccttggccg ccatctcaag gtaagccgcc agtccagcca
     1801 tcgtctttgc gtaatacgga tgggaaggag gcagctttga cagcgtcgta tcgggatcct
     1861 gaatgaaact cttcagcgac tcgatcagct tctgggtgaa ctgtcggtgg gccgtcgccg
     1921 gattccgctg ggccatgaac ggatccgcca cgactctgaa acgcatgttc atcgcatatt
     1981 tcatatacgc aaggatgcct agcgattcga cctggccgcg cggcgcggcg tattccttgc
     2041 ggatggctgc cagctttccg acaccttcaa ccagtttcgc caggtcgtcg ccgcttatgc
     2101 gttccggatc ttcgaggatg gatgcgattt cctcggcagt cggtttttcg cggcgatcca
     2161 cgaggcctga aagcttttcc catcccgaaa gatccgacat gccggcaagt atttcacttt
     2221 ccgcatcgac accgggagcg tatgcctcca gtgccgtctt cagttccgga agtccgctgg
     2281 actcgagttc aagaccgtgt gtcggaagcg cctcaagggc cgcgagaagc ttgtcgaaaa
     2341 gatcggtcgt gccggtgtcg tcagtaaaaa ccggctgcca ctcgatcgcg tccatggcgg
     2401 atgtaacagc ctcggaccat tccttcatgc cggcaattga atcctgctgc cgcgcgcgaa
     2461 caaccgtccg cgaggaattc agggcattcc acatctgcat gagcccagat tcaagcggtt
     2521 cggcgccaac ggcctcctgt tcagggctga ctaccgcggt tcttccgccc gcggcgcgtg
     2581 aagcgggcct gtccggcgtg cagcccggca acaggccgga aatggcaaga agagcgggaa
     2641 tcgagaacag aaagagtcga agttgctttg ttgaagggct tccgaacacg tcgccaccag
     2701 gggcttgagg ccgcgaaaac actacatgat gatgcgcttc aggtcaataa tggaccgacc
     2761 ctttcgcaac tggaggttgt tcattcgggc tattgccgaa caaggtttat aatcgtcttc
     2821 aactttttgc cggccggcac cgattgcttg aaggcgcggc ctgatcctgg aggtcatttc
     2881 cgatgacggg aaacttgttt ggcggcaatc cgcttgtact ggaactggtc aggctcgcga
     2941 ttgcggagga catcggaacg ggggatgtca cgaccggcct ggttatcagg cccggcgcac
     3001 aggcttcggg aagcatactt gcaagacaat ccggcattgt tgccgggctt ccggttgtcg
     3061 agatggtatt ttccgagatc gatccggcca tccgtttcga ggccaaggtc gcggacggcc
     3121 aggccgttga accggatcag gttcttgcgg ctgtgtccgg gccggccgcg tccctgctgc
     3181 gcgccgagcg caccgtgctg aacttcctga tgcatctctc cggtgtcgcg acccacgcat
     3241 ccaggtttgc aagggcaatc tccggtaccg gaatcaggct tctggacacc cgcaagacga
     3301 cgcctggaaa caggattctg gaaaagtatg ccgtggccgt cggcggcggg atgaatcacc
     3361 ggatgaacct ggcgggcggg gttcttgtca agaacaatca catagcggtg tgcggcggag
     3421 tcgaacaggc ggtcggactt gcacgcgcgg gtgccccggt aacgctcaag attgagattg
     3481 aggtcagatc cgtgtcggag gcggtcaggg ccgccagggc cggcgcggac atgttgctgc
     3541 tggatcatat ggggccgtct gaagcggcgg aggtcaggca ggccgtcggc cctgccgtac
     3601 ttcttgaagt ctcgggtaac atgacccccg aaaaggccgt cgcgtacggt ggaaccggaa
     3661 tcgatttcgt gtccgccgga tccgtcgtct atggtgccgt ctggcttgat atctccatgt
     3721 acctggagct ttgaaagggg tttgccggat ggatgcggcg aaggtatgcg aaatcctgcg
     3781 ttccggtggc gtcgatgcca ggttcgccgc cgtggtcgaa agcacgcagg acgagtgcat
     3841 ccgggccggt cgcggtggtg ccccgtccgg aagccttgtg cttgcctcgt cccaggttgc
     3901 cggtcgcggg cgcatggggc gtcgctggtt gtcgccggcc ggagggctgt atgccagcat
     3961 catgcttcga ccggctcttc gacaggaact gtggtcggcg ctgaccctgc acgccggcct
     4021 ggccgtggtt tccgttgttc gtgccgccgg cgttgccgac gcaaggctga agtggccgaa
     4081 cgattgcctc gcgggggggc gcaagttgtg cggaatcctt gcggagtcgt ttccgggtga
     4141 cgggattgtg gtgatcgggc ttggcatgaa tatcgaatcc gccccggacg tcggttcggt
     4201 cgaatgtctg caccacggcc tgccatccgc ctttcttgac ggacttgtcg atgtcgatca
     4261 cgacgtcttc tgtgccgatc tgctgtttcg gatgcggtcg gcccttcttc gggcggattt
     4321 cgggcttcct cttgacaccc gggcgataag tgaaatgctt tgggacggtg gcacgatcaa
     4381 cgcatgctgc gacggttccg aattcagcgg ccgggcaacc ggggtggatc cgctgggaag
     4441 gttgattgtc cttttgcccg acggttcagt acgtcatgtt tcaaccggcg aggtctgata
     4501 tgctggtcgc gattgatacg ggaaacacga ataccgtctt cggaatctac caggggtctg
     4561 acctgaaggc ttccttccgg gtttcctcca ggcgtgacgc gactcttgac gagtggttgt
     4621 cgtcgattga cgggttgctt tcattcaagg gaatcgacag gcggatggtt gattccgcat
     4681 gcatcgcgag tgtcgtcccg tctgtcggtt cgatgatgga ggacgttgtt cgatctctct
     4741 tttccgtcga gcccctggtt gtgggccctg gcacccgtac cggcatttcg atttgctatg
     4801 aaagtccgtc ggacgtcggt cccgaccgga tagtgaacgc ggttgccgca catgcccgat
     4861 acggaatgaa cacgatcgtc atcgacctgg ggacggcaac gacgttcgat gccgttaccg
     4921 cttcgggtca gtatctgggc ggggtgattg taccgggaat agcgatgggt cttgattcgc
     4981 tttcagcaag gacggcgcgc cttccaaggg tcggttttgc gagaaccggc catgtcatcg
     5041 gccggaatac cgtcgccagc attcaatccg gggcctactg gggactggtt gcgatgctgg
     5101 aagggcttac caggcggatg atgggcgagt tcgaaggtcc cgtcagggtc gtcgcgacag
     5161 gcgggcttgc atccctggtt tgcggcgact gtcctttcat agatgaggtt gacggcgacc
     5221 tgacgctcga agggctgagg atcatctggg acaagaaccg tggccccgcc agggatttcg
     5281 ggacgacggc agatcggggg caacattgaa taagctgttt tgcaaaagag gacttgcggg
     5341 caggatgatc aggtttgcgg ccaccctggc cggtaccggc cttgtcatct ggattcttct
     5401 gttttccggc atttatccgc gtcagaccgt tttcagcctg gcggagtcgc agccggcatc
     5461 ggccctcgag taccggaagg acgaccttgc caggcttcgg ctgctgacaa ggtgcatcaa
     5521 gtacatcaga tccgaatatg tggtgcccga aaaggtcaaa cccctgccga tgcttgccgg
     5581 cgcgctgaag gcggccgagg gcctggttcc ggatctgatg gtttcgttca accgcgatga
     5641 cgtcgagacc gcgacttcgg tagaagtgcg aatcggtgaa gttacgaagg tctttccgtt
     5701 ggagaagacc agtgatcttt accagatgaa ctggaagctg gtcgatatgt tcgaattcat
     5761 ctcggcccac ctgcctgccg acgtgaaggc ggacgaggtc gaatacgccg ccatcaacgg
     5821 gatgcttcag ccgctggatg agcattcggt ctatctgccg cccgaggcat accagcaaat
     5881 gcaacttgac acgcaggggc gcttcggcgg gctggggata gtgatatcga gccgcaacgg
     5941 gtacgtcacg atcatgtcgg tcatggagga caccccggcc tggagggccg ccttgaagag
     6001 cggcgaccag atcgtcgaga tcaatgaaga atcgaccatg aacatgccgt tgaccgatgc
     6061 ggtctccagg cttcgcggcg agcctggcac gaaggtctcg atggttgtgg atcggaaggg
     6121 ctggtcggag cccagaacaa ttacgatcga gcgcgccgaa atcaaggttc agagcgtcaa
     6181 gacccagtcg ctgggcgggg gtgtgggata cgccaggctc gtgcacttcc aggaggacac
     6241 cgccgcggaa ctgtcccgtc aactggagga actccgccgt cgcgggtcgc tgcgcgggct
     6301 tgtccttgac atgcgtgaaa atccgggtgg acttctggat caggccgtcg ccgtcggtga
     6361 tttgttcctg cgttcaggga cgcttgtcgt gaccgagggc gagggaaatc gtttgcgcca
     6421 ggcgttcgac gccgattccg gggaggcttt cgaaaacctg ccactcgtcg tgctggttga
     6481 cggcggaagc gcctcggccg ccgagatcgt ggctggcgct ttgaagtaca acgaccgcgc
     6541 catgcttgtc ggatcgacga ctttcggaaa gggaaccgtc caggtcatgc atgaagtcgg
     6601 tgacggtgcc ttgaagctga cagttgccca gtatcttatc ccgggagatc tttcgattca
     6661 gggggtcggg gttgttcccg acatagagct ggttccaacg tcaatcggaa gaaagaacat
     6721 attgatgggg ctgaccgatt acaagcggtt catggatcct tcaaggcgcc tcgacgcttt
     6781 cggaaaggtg gcctcggaca ttccggtgca tgtgattcca ttcctgatga aatccgagga
     6841 ggagcaggaa gaggaagacg acgtgcttga tgaactgcca atcgaggacg attcgttcaa
     6901 gcgtgatgaa tacatcgatc tggccgcgtc catcgtcgct tcaacggtcg cctttgccga
     6961 cgtttcccgc aagtcgcaat tgaagaacgg cgagacggcc gcacttgact ggaagaacgc
     7021 ccaggacgca aagatttcgg caagcctggt cgaaaagggc gttgactggg cggccggccc
     7081 ggtcgtttcc ggcgcgtcgg tttcgacagg tttttctgcc ggttcggcgc cgcttgcggc
     7141 cgggtcgatg gccgtgatga acatgatggt tacgaacggc gggaaagagc cgcttttccg
     7201 ggtaagggca tccttgaaat cggacaacct ttcgctggac ggtcgtgagt tcatcttcgg
     7261 caggatcggt ccgaatgaaa ccgtcagccg acaggtgcag ttcaaggttc cgcgcgagac
     7321 ctggcagcgc cgcgatcagg tcgagatttc gctgtaccag aatgccgtcg aggcaattcc
     7381 cgggggcacg cgcgtcgagt atgtggagtt cgcccctctt ccaaggccgc gtttcgggtt
     7441 ttccgccatg gttcttgacc gccagggaaa cggcgacgga atcctgaatc ccggcgagaa
     7501 ggccgatgtg gtgtttccga ttgcaaacgt cggatccggc gatgcgccaa gggtcatggc
     7561 aagcataagg aaccgcagcg gagagtctct ttatctgaga accggccggg agacaatccg
     7621 cgagggaatc aaggctggag ccagcggcct ggcaaaattc gctgtcgaaa ggcgcgccgc
     7681 ggattcccct gcaaaggtta ccgggggcca gggcgcgact ggcggagcca aggttgagtt
     7741 ctccatcttc gatctggcct tgagggaata cctggccgag gaaatcgaac ttccatcggc
     7801 atccgagccc ggagccgcgt tcaccgcaaa gcccggaacc atcaggacgg tcgggccggg
     7861 cacaagaatt cttgcagccg cgtcggcgga ttcgatggtt ctggccgagg ttcccgaggg
     7921 aaccacgctt cgttcgacag gattcttccc cggtttcttc aaggttgatc tcgccaacgg
     7981 gttgaccggg tttgtcacaa gcaccgacgt catggaaccg ggtaccgcgt ccggctccga
     8041 cctgctgccc gaaccatccg gaaccgcgca ccttgccccc gatatctcgg tggagtttgt
     8101 tcggggtgag ggttcatccc cggtgctgaa ggtcaggggc aaggtgggct tctcggtcgg
     8161 atccggcgat gtaaggcgca agatcctgat attccgcgaa agcgacaagg tgtatttctg
     8221 ggccggcaag ggcggaagcg acagtcggga agttccgctg gacacgacgg tgaagcttcg
     8281 ccccggccgc aacgcg
//
LOCUS       DAOPPA010000084         8229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9725_length_8229_cov_3.417421, whole genome shotgun sequence.
ACCESSION   DAOPPA010000084 DAOPPA010000000
VERSION     DAOPPA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9725_length_8229_cov_3.417421"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKH54_03915"
     CDS             <1..826
                     /locus_tag="PKH54_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOC99065.1"
                     /translation="FFFNVHNNFIEEVAKFRAARRIWAGIMRDRFGAKNPKSWMLRFH
                     TQTAGSTLTAQQPENNAIRVAIQTLAAVAGGTQSLHTNSRDEALGLPTEDSVRLALRT
                     QQIVAYESGVADTIDPFGGSWAIEAETDRIESEALEYIDEVDRLGGMIAAIEAGFVQK
                     QIQDSAFRAGREIDDGTRRIVGVNCFQMENEVEPDILQISPETEACQVASLEAIRKAR
                     DNDAVAAALAAVKDAAVSGANLFPPVLQAVSAYASLGEICNVMRDVFGEYREEVVL"
     gene            823..1236
                     /locus_tag="PKH54_03920"
     CDS             823..1236
                     /locus_tag="PKH54_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOC99066.1"
                     /translation="MKRRIRILIAKPGLDGHDRGAKFVARSLRDAGAEIIYTGIRQTP
                     EAIAAAAVQEDVDFLGLSCLSGAHNRLFPEVVKLVRELGGQDIVVFGGGVIPKRDWPG
                     LNEAGIRFIFGPGTPMSDVIAVIRENAPADAELAP"
     gene            1249..2199
                     /gene="meaB"
                     /locus_tag="PKH54_03925"
     CDS             1249..2199
                     /gene="meaB"
                     /locus_tag="PKH54_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOC99067.1"
                     /translation="MQPDPEMLVRGIRDRNQQAVARAISIIADRRPGWESLLDAVGKE
                     PRTATFIGITGAPGSGKSTLADAITSFYRAGNRRVGIVAVDPSSPFSGGAILGDRLRM
                     QDHAGDPDVFIRSMASRGRLGGLAPATAETAAILAMAGYDPVIIETVGVGQSEVDVMS
                     VADVVLMVLVPGNGDSIQALKAGVMEIGDLFVLNQADRPGSDRLLAEVNAALRLSGDE
                     DCLSRVFQTVATEGRGVPEVATGIDAFLGSLQSDAEKAASRRERQFLRLVNLAVHDMT
                     DEAFEAYRKSGCAPTEGGTSWSEAVRVATGFLDWLRTRPN"
     gene            2226..2630
                     /gene="mce"
                     /locus_tag="PKH54_03930"
     CDS             2226..2630
                     /gene="mce"
                     /locus_tag="PKH54_03930"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOC99068.1"
                     /translation="MAEKIDHIGIAVADLKAASEAFALLLGVQPGLPEEVPDQKVRVV
                     FFDVGGVHVELLEPTCPESTIAGFIEKRGPGLHHVAFAVNEIESELARLTEGGARLID
                     QTPRIGAHGMKIAFVHPKSSAGVLTELCEKVG"
     gene            2669..4228
                     /locus_tag="PKH54_03935"
     CDS             2669..4228
                     /locus_tag="PKH54_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC99069.1"
                     /translation="MKKELSELMRDLDVKNAQAADSDGKRAAKQHEKGKLTARERIDL
                     VLDPGSFVETDALQEHECVNFGMEKQKFPGDGVVTGFGTVNGRPVYVFSQDFTILGGS
                     LGLAFAKKIMKIQDMALQNGAPIIGINDSGGARIQEGVDALHGYGGIFFRNVRASGVI
                     PQISVIVGPCAGGAVYSPALTDFIFMVNGVGNMFITGPQVIKQVTGEDISQEALGGAK
                     AHAEKSGNTHFVFNDEKACFDGVRTLIELLPSNNMCNPEVVDWGDTIDRMESDLGGLV
                     PTDSRKSYDIHDVISRVVDKGWLMEIQPDFARNMVIGFARLGGSAVGIVANQPAWMAG
                     CLDIDASDKAARFIRFCDAFNIPLVTFVDVPGYLPGKAQEHNGIIRHGAKLLFAFAEA
                     TVPKLTVTLRKSYGGASIAMANKDLGCDFMLAWPQAEIAVMGAEGAAGIIFRKDIQAA
                     AAEDQSRVKAEKIQEYEDLFCNPYVAASKGYIDMVIRPEETRPRLIQALRVLKGKRDT
                     LPPRKHGNEPL"
     gene            4256..4621
                     /locus_tag="PKH54_03940"
     CDS             4256..4621
                     /locus_tag="PKH54_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOC99070.1"
                     /translation="MDELVGVNSAVMISLIAFSVVFLVLGSMALFMYGLGWTVKSLKM
                     LAQGREQVPPDASAARPAGSRGDTDIDPRTVAAVTAAVSVMMEGTRFRVADIRQVGGE
                     ESRWIGSGRLKMPFSAWRR"
     gene            4621..5046
                     /locus_tag="PKH54_03945"
     CDS             4621..5046
                     /locus_tag="PKH54_03945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOC99071.1"
                     /translation="MAVRHFRIKVEGKTFEVDVEELGVTAGVAAAGTTPPVRAVPGAV
                     PASVPPAGTTATSAQAPKPAASTPAAGQGWVLCPMAGKVQKIPVKVGQTVSADTVVAV
                     LEAMKMETSVCAGTAGTVQEIAASEGAVLDSGAALVKVG"
     gene            5099..6208
                     /locus_tag="PKH54_03950"
     CDS             5099..6208
                     /locus_tag="PKH54_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOC99072.1"
                     /translation="MMIIWTGSGFAQLQWGHMLMLVVGITLITLAVKKGFEPLLLIPI
                     GFGAILTNLPGSGIMDPTHGFLGQIYNAGTASEIFPIIMFLCLGALTDFKPLLANPVT
                     FLLGAAAQGGVFVALVGAVALGFSVPAACAIGIIGGADGPTSIYLASKLAPEYLGAIA
                     VASYSYMSLVPLIQPPVIRLLTTKKERSIEMKATREVSRTELVVFPVVVAILCGLLLP
                     PAVPLIGMFMLGNFLRESHVVDRLVNTAQNEICNITTIFLGVTVGATMEADKFLQVAT
                     LKIISLGLVAFIFSTFFGVLFGKLMMVLSGGRINPIIGAAGVSAVPMAARTAHQEGQR
                     ANPRNFLLMHAMGPNVAGVIGTAVAAAVMLALLGG"
     gene            complement(6212..7111)
                     /locus_tag="PKH54_03955"
     CDS             complement(6212..7111)
                     /locus_tag="PKH54_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC99073.1"
                     /translation="MKSYGFIAVCLLLLAGTAAAGQERAGSTESPSPVERAENVPENV
                     SPWELHPALETGLLIGGLTMMSLDFVYFRQNRGPSCGISCDPAGINRLDRAVAGWWNP
                     AAATASDVTLSVSIALPFLAQAIDTAVSRPSDGWKGYGRDVLVLVETAAVTSGLTSLV
                     KYSVGRPRPYSYNRDLDESKRTERDAGLSFPSGHTSMAFAMATSWSWLYMKRHPRSPG
                     VVPVWIGSYALALTTAVLRPVAGKHFWTDIIAGAGLGIGVGLLVPYLHQVVAKKKRQS
                     KTVVTVVPAIVEGGGAAMVTVIR"
     gene            complement(7125..7829)
                     /locus_tag="PKH54_03960"
     CDS             complement(7125..7829)
                     /locus_tag="PKH54_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99074.1"
                     /translation="MTRMNFAAMVVAASITWLYAFDATGAEPAVEPADTSSIEGEGSS
                     SADRSVPPAASEDEGQATAAWKWSPCNFDTFVKKVFVVVDVHASLLSDAEDRKPLGDS
                     FGYAVKAGWRFGRLGVFASFEHNLWIRSELKSGVSAGVANIGIGLEVNYLHGYARSSL
                     AIGPSVLLFDTGIDQAGSTGFFMDLRPIGLRWPITRFFALTLDPISFALVAPALDRVP
                     LVTVQFRTTVGFEFVL"
     gene            complement(7826..>8229)
                     /locus_tag="PKH54_03965"
     CDS             complement(7826..>8229)
                     /locus_tag="PKH54_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99075.1"
                     /translation="AMVGQMLAEQVSPMFLQQVDALRRSAIARREQAAPVAEVAAEAV
                     AELVSAVAIQASGRSGSAVGEVLERWLHLQPGCNETNDWCNSPWIELVRQEPFMPILD
                     MAGGCGASGGSAAGLLLLLPVAVLSRIRRRR"
BASE COUNT         1696 a         2413 c         2565 g         1555 t
ORIGIN      
        1 gttcttcttc aacgttcaca acaacttcat cgaggaagtc gccaagttca gggccgccag
       61 gaggatctgg gccggtatca tgcgcgatcg tttcggggca aagaacccga agtcgtggat
      121 gctgcggttc cacacgcaga ccgccgggag cacactgacg gcacagcagc ctgaaaacaa
      181 cgcgatccgg gtcgccatcc agacgctggc cgcagttgcc ggtggaaccc agagcctgca
      241 cacgaactcg cgtgacgagg cgctcggact tccgaccgag gactcggtgc ggctggccct
      301 gcggacgcag cagatcgtcg cctatgaaag cggcgtcgcc gacactatcg acccgttcgg
      361 cggaagttgg gccatcgagg cggaaaccga tcgaatcgaa tccgaggccc ttgaatatat
      421 tgacgaagtt gatcgacttg gcggcatgat cgccgcgatt gaagccggtt tcgtccagaa
      481 gcagattcag gattcggcct tcagggctgg acgcgaaatc gacgacggca cccgcaggat
      541 tgtcggcgtc aactgcttcc agatggaaaa cgaggtcgag ccggacatcc ttcagattag
      601 ccctgaaacc gaggcttgcc aggttgcatc gcttgaggcg attcgaaagg cccgcgacaa
      661 cgatgccgtc gcggctgccc ttgcggccgt caaggatgcg gccgtttcgg gcgccaacct
      721 gttcccgccc gttcttcagg ccgtgtctgc gtacgcaagc cttggggaaa tctgcaacgt
      781 catgcgggac gtattcggcg aatacaggga agaggtggta ctgtgaagcg cagaatcagg
      841 atattgattg cgaagccggg cctggatgga cacgaccgcg gggcgaaatt cgttgcccga
      901 tcgcttcggg acgcaggcgc ggagatcatc tacacgggca tcaggcagac gccggaagcg
      961 attgccgcgg ccgcggtcca ggaagatgtc gattttcttg gattaagctg cctttcgggc
     1021 gcgcacaatc ggctctttcc ggaagttgtc aagctggtca gggaacttgg cgggcaggac
     1081 attgtcgttt tcggcggcgg cgtgattccg aaaagggact ggcccggcct gaacgaagca
     1141 gggataagat tcatcttcgg acctggaaca ccgatgtccg acgtaatcgc ggtcatacgg
     1201 gaaaacgccc cggccgacgc cgaactggcg ccctgaaggt cttttgcgat gcaacccgat
     1261 cccgaaatgc tcgtccgggg aatccgcgac cgcaatcaac aggcggtggc ccgggcgatc
     1321 tccatcattg ccgacagaag accgggatgg gaatccctgc ttgatgcggt tggaaaggaa
     1381 ccgcggaccg cgactttcat cgggattact ggcgcgccag gctccggcaa atcaacactt
     1441 gcggacgcca taacctcgtt ttacagggcc ggaaaccgac gtgtcggaat cgtggcagtc
     1501 gatccgtcca gcccgttttc cggcggagca atccttggcg acagacttcg gatgcaggat
     1561 cacgccggag atccggatgt cttcatcaga agcatggcgt cgcgcggcag gctcggtggc
     1621 ctggcgcccg ccacggccga aaccgcggcc atactcgcga tggccggtta cgatccggtc
     1681 atcatcgaaa cggtcggcgt cggacagtcc gaagtcgatg tgatgtcggt ggccgacgtc
     1741 gtgctgatgg tgctggtgcc gggaaacggt gactcgattc aggcgctgaa agccggcgtg
     1801 atggaaatcg gcgacctgtt tgtcctgaac caggcggatc gaccgggatc ggatcggctg
     1861 ctggccgagg tcaacgcggc attgcggctg tcgggcgacg aggattgcct ttccagggta
     1921 ttccagaccg tcgcaaccga gggtcgtggg gttccggaag tggcgacagg catcgacgcc
     1981 ttcctgggca gccttcaatc cgatgcggaa aaggctgcgt cgcgtcgcga gaggcagttt
     2041 ttgcggcttg tcaatcttgc cgtacatgat atgacagacg aggcgttcga ggcgtaccgg
     2101 aagtccggat gcgccccgac cgagggcggc acgtcctggt ccgaagccgt cagggttgcc
     2161 accggtttcc ttgactggtt gagaaccagg ccgaactgaa aattggatgt caccggagca
     2221 ttcgaatggc ggaaaaaatt gaccacatcg gtatcgcggt tgcagatctg aaggcggcat
     2281 cggaggcatt cgcccttctg ctgggcgttc aacccggcct tccggaggag gttccggacc
     2341 agaaggtgcg cgtggtgttt ttcgatgtcg gcggcgtgca cgtcgaactg cttgagccga
     2401 cctgccccga aagcaccatc gccggtttca tcgaaaaacg cggccccggg ctgcaccatg
     2461 tcgcgttcgc ggtcaacgaa atcgaatccg agctcgcgcg cttgaccgag ggcggtgccc
     2521 gactgatcga ccagacaccg cgcatcggtg cgcacggaat gaagattgct ttcgtccatc
     2581 cgaagtcatc ggccggcgtt ctgacggagc tttgcgagaa ggtcggctga agccgtaaca
     2641 aaaaggttca atctgaaggg tttcacagat gaagaaagaa ctttccgaat tgatgcggga
     2701 tctcgacgtc aagaacgccc aggcggccga ctccgacggg aagcgggcgg cgaaacagca
     2761 cgagaagggc aagctcacgg cacgtgaacg gattgacctt gtcctggatc cgggcagctt
     2821 cgttgaaacc gacgcccttc aggaacacga atgcgtcaac ttcggcatgg aaaagcagaa
     2881 gttccccggc gacggtgtcg tgaccggatt tggaaccgtc aacggccgtc cggtctatgt
     2941 cttttcgcag gacttcacga tcctgggcgg atcgctcggg ctggccttcg cgaaaaagat
     3001 catgaagatc caggacatgg cgctgcagaa tggcgctcca atcatcggga tcaacgattc
     3061 cggcggggcg agaatccagg aaggagtcga tgcccttcac ggttacggcg gaatcttctt
     3121 caggaacgta cgggcatccg gcgtcatccc gcagatatcg gtgattgtcg ggccgtgcgc
     3181 cggcggtgcc gtgtattcgc ccgccctgac agatttcatc ttcatggtca acggcgtcgg
     3241 caacatgttc atcacggggc cgcaggtcat caagcaggtc acgggcgagg acatctccca
     3301 ggaggctctt gggggcgcga aggcacacgc ggaaaagagc ggtaacacgc atttcgtgtt
     3361 caacgacgag aaggcctgct tcgatggcgt ccgtacgctt atcgaactgc tgccttcaaa
     3421 caacatgtgc aatccggaag ttgttgactg gggcgatacc atcgatcgga tggaatccga
     3481 tcttggcggc ctggttccga ccgacagccg gaagtcatac gacatccatg acgtgatttc
     3541 ccgggtggtc gacaagggat ggctgatgga aatccagccc gacttcgcca ggaacatggt
     3601 gatcgggttt gccaggcttg gcggctcggc agtcgggatt gttgcgaacc agcctgcatg
     3661 gatggcggga tgcctggata tcgacgcctc cgacaaggcg gcccgcttca ttcggttctg
     3721 cgacgccttc aacattccgc tggtcacgtt cgtcgatgtt cccggctacc tgccgggcaa
     3781 ggcccaggag cacaacggaa tcatccgcca tggcgcgaag ctccttttcg cgttcgcgga
     3841 ggcgaccgtc ccgaagttga ccgtcacgtt gcggaaatct tacggcggcg catcgatcgc
     3901 gatggccaac aaggatcttg gctgtgactt catgctggcc tggccacagg ccgaaatcgc
     3961 ggttatgggc gccgagggcg cggccggcat catattcagg aaagatatcc aggcggccgc
     4021 tgcggaagac cagtccaggg tcaaggcgga aaagatccag gaatacgagg atcttttctg
     4081 caatccctac gtcgcggcat ccaagggcta tatcgacatg gtcatcaggc ccgaggagac
     4141 caggccgcga ctgattcaag ccctgcgcgt cctgaaagga aaacgggaca ctctgccgcc
     4201 aaggaagcac ggcaacgagc ccctctgatt acctttttgg caaggtggtg aaagaatgga
     4261 cgaacttgtc ggcgtcaact ccgcggtgat gatatcgctc atcgcattct ccgtcgtctt
     4321 cctggtgctt ggatcgatgg ccctgttcat gtacggactt ggctggacgg tcaagagcct
     4381 gaagatgctg gcccaggggc gcgaacaagt gccgcctgac gcttccgctg ccaggccggc
     4441 cggatcgcgg ggcgacacgg acattgatcc ccggaccgtt gcggcggtta cggccgcggt
     4501 atcggtgatg atggaaggaa cccgtttccg ggtggcggat atcagacagg tcggcggcga
     4561 ggaatcgcgc tggatcggat cgggccgttt gaagatgccc ttttctgcat ggagaagatg
     4621 atggctgtaa gacatttcag gataaaggtc gaaggaaaga cgtttgaagt cgatgtcgag
     4681 gaactgggcg taacggccgg tgtggccgca gccgggacaa ctcccccagt cagggccgtt
     4741 cccggtgccg tgcctgcgtc agtcccgcca gcggggacga ccgcgacgtc agcccaggcg
     4801 ccaaaacctg ccgcttcgac gcctgcggcc ggccagggat gggttttgtg ccccatggcg
     4861 ggaaaagtcc agaagatccc ggtcaaagtc ggccagaccg tttcggccga tacggtcgtt
     4921 gcggttctcg aggcgatgaa gatggagact tcggtttgcg cgggcacggc cggcacagtc
     4981 caggagattg ccgcgtccga aggggctgtt cttgacagcg gagcggcgct ggtaaaggtc
     5041 ggctgaacca gcatgaaccc tttcgaaggt atctgaagtg agcgacatac ttgccagaat
     5101 gatgatcatc tggactggtt ccggttttgc ccagttgcag tggggccaca tgctgatgct
     5161 tgtggtcgga atcacgctta ttacgttggc cgtcaagaag ggattcgagc cccttctgct
     5221 gatccccata ggctttgggg cgatactgac caatcttccg ggatccggca tcatggatcc
     5281 gacccacgga ttcctgggac agatttataa cgccggcacc gccagcgaaa tttttccgat
     5341 catcatgttc ctgtgcctcg gcgcccttac cgatttcaag cccctgctgg cgaacccggt
     5401 cacgttcctg ctgggcgcgg ccgcccaggg gggggtcttc gtcgcgcttg tcggtgcggt
     5461 tgcacttggc ttcagcgtgc ccgcggcatg cgccatcggg atcatcggcg gggccgacgg
     5521 accgacatcg atctacctgg ccagcaagct cgcaccggag taccttggcg cgattgcggt
     5581 tgcaagttac tcctatatgt cgctggtgcc gctgatccag ccgcccgtaa tcaggctgct
     5641 gaccacgaaa aaggaacgct cgatcgagat gaaggccacg cgcgaagtca gccgcacgga
     5701 actggtcgtc tttccggtgg tcgtcgctat cctctgcggc ctgcttcttc cgccggcggt
     5761 gccgcttatc gggatgttca tgcttggcaa cttcctgaga gagtcacatg tcgttgacag
     5821 gctggtcaac acggcccaga acgagatctg caacatcacc accatattcc ttggcgtaac
     5881 cgtgggcgcg acgatggagg ctgacaagtt tcttcaagtg gcgacattga agattatctc
     5941 ccttggactt gtggcgttca tattctcgac ctttttcggc gtccttttcg gaaagctgat
     6001 gatggttctt tccggcggaa gaattaaccc gataatcggc gccgcggggg tttccgcggt
     6061 tccaatggcc gcaagaaccg cccatcagga aggccagcgc gccaacccac gaaacttcct
     6121 gctgatgcac gcgatgggcc cgaacgttgc cggcgtaatc gggacggcgg ttgcggccgc
     6181 ggtgatgctt gcacttcttg gcgggtgacc gtcacctgat aacggtgacc atggctgcgc
     6241 ccccgccttc aacaattgcg ggaacaacgg taacgacggt ttttgactgc ctcttcttct
     6301 tcgcgacgac ctgatgaagg tatggaacca gcagcccgac gccgataccc agccccgcgc
     6361 ctgcgatgat gtcggtccag aagtgcttgc ccgccaccgg ccgcaggaca gcggtcgtca
     6421 gggccagcgc atagcttccg atccagaccg gaacgacgcc gggacttcgc gggtgacgct
     6481 tcatgtaaag ccatgaccag cttgtggcca tggcaaaggc catcgaggta tgtccggaag
     6541 gaaaggacag tccggcgtcc ctttccgtcc ttttcgattc gtccagatca cggttatatg
     6601 aatacggacg gggacgaccg accgaatact tgaccaggct tgtaaggccg gaagtgaccg
     6661 cggcagtctc gacaagaaca agcacgtccc gtccgtatcc cttccagccg tccgacggcc
     6721 tggacacggc cgtgtcaatc gcctgggcca ggaacggaag cgcaatcgat acggaaagcg
     6781 tcacgtccga cgcggttgcc gcggcaggat tccaccaccc tgcaacggca cgatcaagcc
     6841 tgttgattcc ggccggatca caggaaatcc cgcacgacgg gccacgattc tgcctgaaat
     6901 acacgaagtc caggctcatc atggtcaaac ccccgatcaa cagcccggtc tccagcgccg
     6961 gatggagttc ccacggactg acgttttcgg ggacattttc ggccctttcc accggggacg
     7021 gggattcggt tgaaccggcc cgctcctgac ccgccgcggc ggtgccggcg agcaacagca
     7081 ggcagacggc gataaacccg tacgacttca atcagggccc cctttcacag aacgaactcg
     7141 aatcccacgg tcgtcctgaa ctgaaccgtt accaggggaa cacgatccag ggcgggggcg
     7201 accagggcaa aggaaatcgg gtcaagggtc agggcaaaga atctcgtaat cggccagcgc
     7261 aggccaatgg gccgcaaatc catgaagaat ccggtactcc cggcctggtc tattcccgta
     7321 tcgaaaagca ggacggatgg gccgattgcc aggcttgagc gcgcatagcc gtgaagataa
     7381 ttgacttcaa gcccgatgcc gatgttggcc acgccggcgg aaaccccgga tttcagttcg
     7441 gacctgatcc acaggttatg ttcaaatgat gcaaacacgc caaggcggcc gaaacgccag
     7501 ccggccttga ccgcgtaacc gaagctgtcg ccaagcggtt tgcgatcctc ggcgtcgctg
     7561 agcagtgaag catggacatc gacaaccacg aagaccttct tgacgaaggt atcgaagttg
     7621 cagggggacc acttccatgc cgcggtcgcc tgtccttcat cttctgatgc agcgggcggg
     7681 acgcttctgt cggcggagga cgaaccctcc ccttcgatcg aagacgtgtc agccggctcg
     7741 acagccggtt ccgcgcccgt cgcatcaaag gcataaagcc aggtaatcga tgcggcgaca
     7801 accatggccg cgaaattcat tcgcgtcatc gacgcctcct gatgcgggaa aggacagcta
     7861 cgggaagcag cagcagcaga cccgccgcgc tgccaccgga agcaccgcag ccgccggcca
     7921 tgtcaagaat cggcatgaat ggttcctgcc ggacaagctc gatccacgga ctgttgcacc
     7981 agtcgtttgt ctcgttgcat cctggttgga ggtgaagcca cctttcaagc acttccccga
     8041 cggcggaacc ggatcggccg gaagcctgga tcgccacggc cgagaccagt tccgcaactg
     8101 cctccgcagc aacttcggcc accggcgcgg cctgctctcg ccgtgcaatc gcactgcggc
     8161 gcagggcatc cacctgctgc aaaaacatcg gcgacacttg ttcggccaac atctgcccga
     8221 ccatcgcgc
//
LOCUS       DAOPPA010000085         8167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9815_length_8167_cov_4.169872, whole genome shotgun sequence.
ACCESSION   DAOPPA010000085 DAOPPA010000000
VERSION     DAOPPA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_8167_cov_4.169872"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /gene="rpoD"
                     /locus_tag="PKH54_03970"
     CDS             <1..1619
                     /gene="rpoD"
                     /locus_tag="PKH54_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOC99076.1"
                     /translation="DIDDVLVELRTAGVEVVEGGASKGSSKMKDSVSIPIDEDSYSEA
                     YGKSNDPVRMYLRRMGEVPLLNREGEVEIARRIEEGELEVFGLIMETDIAIAEILKLE
                     ESLRRGRVSIKQIVKDLDEDEEVGDEVATRKRVLDLLDQLRELDKVIHREMMLLEKPN
                     QSARAKQQLLAQLAGHKEDMKMALAAMRLNQSQINEIVEILKDCVRKINMARCEIRAV
                     EREVGDLPALRRMLRDSKLSPDRVACSDSGRTFSIEQLEEMDHKVQNARKRIRAVEQK
                     ANLSADQLLCTQQAIMSAEKRTEKAKWELVEANLRLVVSIAKKYTNRGLQFLDLIQEG
                     NIGLMKAVEKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRT
                     SRYLVQEYGREPTPEEISEKMEIPVEKVRKVMKIAREPISLETPIGEEDDSRLGDFIE
                     DKKTPSPADSVVKANLAEQMRKVLCGLATREEKVLRMRFGIGERSDHTLEEVGQEFKV
                     TRERIRQIEAKALRKLRHPSRSKKLKTFLE"
     gene            1641..2918
                     /locus_tag="PKH54_03975"
     CDS             1641..2918
                     /locus_tag="PKH54_03975"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOC99077.1"
                     /translation="MDDVLKLGFLGGLQRAGIVPGLDRMRALVERLGHPQSRYPVIIV
                     TGTNGKGSTTCFIDSILRAAGFRTGRFTSPHLVDVRERVFIDGRMVDAGTFARLGEEL
                     RRVLQKESLEVTFFEALSAIGFMAFAEAGVDVAVVEVGMGGRLDSTNVSDPVVSVLTS
                     VGLDHTSFLGSTVEAIALEKTGVARPGRPFITGVEDGLYGSTVGPELERIGARPVRYG
                     LDFTCREGVDGLDWESGGRVHRALVPGLSGRYQSFNLCLAVAAVEAFLAVSGRTANES
                     AYVKGARDAFWPGRFQMVARDPVVILDGCHNVHGARALKNELSSLSRPLVMVHGTRPE
                     KDYDGVLALLAPEADAIVHAGFEGGADPHSVAAVSVAHVRPGVAVMVEPCLSDAVAKA
                     VALAGKSGTVLVTGSLYMIGAAIRDFGWPGFAD"
     gene            2926..3549
                     /locus_tag="PKH54_03980"
     CDS             2926..3549
                     /locus_tag="PKH54_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOC99078.1"
                     /translation="MDFLPAGIEETGDVPGNPGTPGNPMTIMMVLAPGDFNDDEYSIC
                     REVFDAAASGGIATGLVPGTSLDVRVCGLKKGLVRGSFGLWVDVELALFEVDLRSCAA
                     VVFIGGSGVSACLNSRFILKLARAAVDAGCVVGALCAAPGILARAGLLSMRPATAHPV
                     AADELAMGGAVIVDSPVVVSERIVTAAGPFAAREFAERIVAMARLGR"
     gene            3599..4048
                     /locus_tag="PKH54_03985"
     CDS             3599..4048
                     /locus_tag="PKH54_03985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC99079.1"
                     /translation="MRSYFLVALSSVLVVSFAAGCKEEPAKEAKVEKAESAEVTTPSG
                     LKYVDIKVGEGAVPQAGQTVIVHYTGTLTDGRKFDSSVDRGQPFTFPLGQGRVIKGWD
                     EGLSTMKIGGKRKLIIPPELGYGARGAGGVIPPNATLVFDVELLGVR"
     gene            complement(4058..5314)
                     /locus_tag="PKH54_03990"
     CDS             complement(4058..5314)
                     /locus_tag="PKH54_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99080.1"
                     /translation="MDRLAEGTVPHLDLPNDNPALKGARRIARMLSAAGFEAWLAGGS
                     VRDLLMGRTPMDFDIATSARPEQVIAIFDRTIPVGVRFGVVIVRMYGFDYEVATFRSD
                     IGHSDGRRPDDVRFTDLHEDVKRRDFTMNGIVCDPVSGCVVDLVGGIDDIRNGVIRAI
                     GDPAERFREDRLRQMRAIRFAAQTGFSIEPATLAAVAENAPFIVKVSAERIAEEYRKL
                     LESGDPARGLELAARTGTLNHTIPELSADAAGSAVELARRLSGGPIELMWAAVLQSLD
                     PQKAGDVLRRLRRSNAMIDSVVAILSDAREAEAGPSGDVAAEKRMIRRKSFADGLLLL
                     QAVLEATGRSIDPVLAWRERAAQWKQDELFPEPLVSGNDIIAAGVARGPAIAALLDDV
                     ESQQLRNELVTRNDALNYLAGLPKIR"
     gene            complement(5314..6297)
                     /locus_tag="PKH54_03995"
     CDS             complement(5314..6297)
                     /locus_tag="PKH54_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOC99081.1"
                     /translation="MHSDMNPIRLGVITNPAAMHNERFPYTHKRLEKRLAQAGDAVLT
                     ASLGEIDDAVRTLLVDRGINVLGINGGDGTIHSTIGAVIGLLQANPGIARPTLLLLNG
                     GTYNIASRAMRTNHNPVDTLRRFQARWGGQPVEKVSTRELGLLEVRTEEGRTLTGMVF
                     GSQVIANALELCDEIGGGYSGFSRFLLRGVAGYAFKTPFFRENKWRLQHRADPIIVDS
                     TAGNRTVGVVASTVDVKLARGLVWALSASSPEEGFHSKTISAGNPSDVVRLVPFLLWE
                     LPHPMIVARPRTKMIRTSGNFTLDGELYPDMGPITVTISPWRFNVVAGEDL"
     gene            complement(6320..>8167)
                     /locus_tag="PKH54_04000"
     CDS             complement(6320..>8167)
                     /locus_tag="PKH54_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99082.1"
                     /translation="GTDEEVACVSGFGLVGEPEDPLLDADIVLSGNQLRNVEIFYRDC
                     LGDDADRLVTFKQIDGEDVCSLQVQMDYTDKNGIALMPVQSDVTSGFCHIEACPDGAA
                     DQCITVTIYVEPKKIPPLRVRFDQYDGRFNVTNGSGTVRLYKKTPENQYPCSAMDPRN
                     LPSPATVEKADVAIIGGLAVFSRLEGLGNEAGQVLSQDYTIVGLAYEAQDNRNIRAWA
                     CDDTGGHVEFGDMTTVILKLYDVVPSIFGEWDIDSNFDLISGLPPTVEKIINILIGLF
                     VSPTGQILLLMCDESLLGFTVGETFCGYIFADPKNPKLGEYGTIGSFVINIVDQIIVN
                     LLKNNCPYEDDPDLCSKIYFTGRDVGMILQKFRILSTMNCTKEPVMDWSDPDAGAIIS
                     KGDCTENWHTVVFRWSLGEDCDPADMECGAKYFSMSSIPGIGKAISAEISGRITEGQY
                     LHIDQHAVNLKYGALINFALEKILLPQVFGDGSDGLPAVDTYEKLVGSLLAGRQCLRT
                     MSCCADFDATLTQKYTWLPAGLAGGACEALLDTAVGFIRDQLNLLDATPENFTLGTPE
                     DDPAQMFDTNNDMKFDTIGNQLDLANWEARLQIGSSVYDPIGLFWGIRN"
BASE COUNT         1543 a         2332 c         2625 g         1667 t
ORIGIN      
        1 aggacatcga cgacgttctg gttgaactca ggacggccgg cgtcgaggtg gtcgaggggg
       61 gcgcttcgaa gggttcctcg aagatgaagg attcggtctc cattcccata gatgaagatt
      121 cctattcgga agcttacggc aagtcgaatg acccggtcag gatgtacctg cgccgcatgg
      181 gcgaggttcc gcttctgaat cgtgagggcg aggtcgaaat cgcccgcagg atcgaggagg
      241 gcgaactcga ggtcttcggt ctgataatgg agaccgacat cgccatcgcc gagatactga
      301 agctcgagga aagccttcgg cgcgggcgcg tcagcatcaa gcagatcgtc aaggatctgg
      361 acgaggacga ggaagtcggg gacgaggttg ccacgcgcaa gcgcgtcctg gatctgctcg
      421 atcagcttcg tgaactggac aaggtgattc atcgcgaaat gatgctgctt gagaagccga
      481 atcagtcggc gcgggcaaaa cagcagcttc tggctcagct tgccggccac aaggaagaca
      541 tgaaaatggc cctggcggcg atgcgcctga accagtccca gattaacgag attgttgaaa
      601 tcctgaagga ctgcgtcagg aagatcaaca tggccagatg cgagattcgc gccgtggaaa
      661 gggaggtcgg ggatctgccg gcccttcgcc ggatgctgcg cgactcgaaa ctgtccccgg
      721 atcgcgtggc ttgttcggac tctggacgaa ccttctccat cgagcagctc gaggagatgg
      781 atcacaaggt ccagaacgcc aggaagagga tccgggcagt cgagcagaag gccaacctga
      841 gcgcggatca gcttctgtgc acacagcagg cgatcatgtc ggccgagaag cggaccgaga
      901 aggccaaatg ggaactggtc gaggccaacc tgaggctggt cgtgtccatt gccaagaagt
      961 acacgaatcg cgggttgcag ttccttgacc tgatccagga aggtaatatc gggctgatga
     1021 aggcggtcga gaagttcgag taccgccgcg gctacaagtt ctcgacgtac gcgacatggt
     1081 ggatacgcca ggcgatcacc agggcaatcg cggatcaggc aagaacgatc agaatcccgg
     1141 ttcacatgat cgagaccatc aacaagctgg tccggacaag ccgatacctg gtccaggagt
     1201 acggccgcga gccgactccc gaggagatca gcgagaagat ggaaattccg gtcgagaagg
     1261 ttcgcaaggt gatgaagatc gccagggaac cgatttcgct ggaaacgccc atcggcgagg
     1321 aagacgactc caggctcggg gatttcatcg aggacaagaa gacgccgtct cccgccgatt
     1381 cggtcgtgaa ggccaatctt gccgaacaga tgaggaaggt tctgtgcgga cttgccaccc
     1441 gcgaggaaaa ggtgcttcgc atgcgcttcg ggattgggga acgcagcgac cacaccctcg
     1501 aggaagtcgg ccaggagttc aaggttaccc gcgagcgtat ccgccagatc gaggcgaagg
     1561 ccctgcgcaa gctccgccat ccgtcccgtt cgaagaagct caagactttc cttgagtagc
     1621 caagaggatc ctgacacatc atggatgatg tcctgaagct cggattcctg ggcggcctgc
     1681 agcgtgccgg catcgtcccc ggtctggatc ggatgcgcgc gctggtcgag cgtcttggac
     1741 acccgcagtc gcgctacccc gtcatcattg tcacgggcac aaacggaaag ggctcaacca
     1801 cctgtttcat cgattcaatc ctgcgggcgg ccggcttcag gactgggcga ttcacctcgc
     1861 cgcacctggt cgatgtacgg gagcgggtct tcatcgacgg caggatggtt gacgccggga
     1921 ctttcgcgag gctcggcgag gaactcaggc gggtgctgca gaaggaatcg cttgaggtca
     1981 cattcttcga ggcgctttcg gcgattggtt tcatggcgtt tgccgaggct ggcgtcgatg
     2041 tcgcagtcgt cgaggtcgga atgggcggaa ggctggattc cacgaatgtt tccgacccgg
     2101 tggtttccgt cctgacatcg gtcggactgg atcatacgtc gttccttggt tcgaccgtcg
     2161 aagccatagc ccttgagaag actggcgtgg caaggccggg gcggcctttc ataaccggtg
     2221 tcgaagacgg gctctacggt tcaaccgtcg gcccggagct tgaaagaatc ggcgcccgcc
     2281 cggttcgata cgggctggat ttcacgtgtc gcgaaggtgt tgacggactt gactgggaat
     2341 ctggcggtcg cgttcaccgc gccctggttc ccggtctttc cggcaggtac cagtcgttca
     2401 acctgtgcct ggccgtggcc gcggtcgagg ccttcctggc cgtttcggga cggacggcga
     2461 atgaatccgc gtatgtaaaa ggtgcgcgag atgccttctg gccggggcgt tttcagatgg
     2521 ttgcccgcga cccggtcgtg attcttgacg gctgtcacaa cgtccacggg gcgcgggctc
     2581 tgaagaacga gctttcatcg ctgtcgcgtc cgctggtcat ggttcatggc accaggcccg
     2641 aaaaggatta tgacggcgtt ctggcgctgc ttgcgccgga agcggatgcg attgtgcatg
     2701 ccgggtttga aggcggcgcc gacccccatt ccgtggcggc cgtttcggtg gcgcacgtgc
     2761 gacctggcgt ggcggtcatg gtcgagccgt gcctgtccga tgctgttgca aaggcagtgg
     2821 cacttgcggg aaaatccggg actgtccttg taacgggctc cctttacatg attggcgccg
     2881 ccatccggga tttcggctgg ccaggcttcg cggattgagg ttcggatgga tttcctgccg
     2941 gctggaatcg aagaaaccgg ggatgtcccg ggtaatcccg gaacgcctgg aaacccgatg
     3001 accataatga tggttctggc ccccggcgac ttcaacgacg acgagtattc aatctgccgc
     3061 gaagttttcg atgcggctgc atccggaggc atcgcgaccg gtctggttcc ggggacaagc
     3121 ctggatgtga gggtctgcgg cctgaagaag ggccttgtcc gtggaagttt cggactatgg
     3181 gtcgatgttg aactggcgtt gttcgaagtc gatttgcggt cgtgtgccgc tgttgttttc
     3241 attggcggtt ccggcgtgtc cgcgtgcctg aacagtcgtt ttatccttaa gcttgcccgg
     3301 gcggctgtcg atgccggttg cgtcgtcggg gccctttgcg cggcccccgg catcctggcc
     3361 cgcgcgggac tgctgagcat gcggccggca acggcgcatc cggtggccgc ggatgaactg
     3421 gccatgggcg gggcagtaat tgttgacagc ccggtggtcg tttcggaaag gatcgtgacg
     3481 gcggccgggc ctttcgcggc gcgtgaattc gcggagcgta tcgtggcgat ggcaaggctg
     3541 ggccgctgac gaacgcggcg gtctgttttt cggggatcgt gccccggtgg aggttcacat
     3601 gaggtcgtat ttcctggtgg ctctgtcgtc ggtacttgtg gtttcgtttg cggccggttg
     3661 caaggaagaa ccggcgaagg aggcaaaggt ggaaaaggct gaaagtgcgg aagtcacgac
     3721 cccgagcggt ctgaagtacg ttgacatcaa ggtgggcgag ggtgccgttc cccaggcggg
     3781 acagactgtg atcgtgcatt acaccggcac gctgacggat ggcaggaagt ttgacagttc
     3841 ggtcgatcgt ggacagccgt ttaccttccc gcttggccag gggcgcgtga tcaagggttg
     3901 ggacgaaggt ctttcaacga tgaagattgg tggaaagcgc aagctgatca tcccgccgga
     3961 gcttggatac ggagcgcgcg gagccggcgg tgtaattccc ccgaacgcca ccctggtttt
     4021 cgatgtggaa ctccttggcg tcaggtgaaa ttccgtttca gcgtatcttc ggaagtccgg
     4081 caagataatt cagggcatcg tttcgtgtca ccagctcgtt tcgaagctgc tggctttcca
     4141 cgtcgtccag aagcgcggca atcgccgggc cccttgcaac ccctgccgcg attatgtcgt
     4201 tgccggaaac cagcggttcg gggaacagtt cgtcctgctt ccattgagcg gcacgctcgc
     4261 gccacgccag gacgggatcg attgaacgcc cggtcgcttc cagcaccgcc tgaagcagca
     4321 gcaggccgtc agcgaaggat ttgcgccgta tcatcctctt ttcggccgcc acgtctcccg
     4381 atgggccggc ttctgcttcg cgggcatcgg acaggatggc gacgaccgaa tcgatcatcg
     4441 cgttgcttcg tcgcagcctc cggagaacgt cgccggcctt ctgcgggtcc agcgactgga
     4501 gcacggccgc ccacatcagt tcaatgggcc caccgcttaa ccggcgggca agctcgacgg
     4561 ccgagcccgc cgcatccgcg gaaagttccg gtatggtgtg attcagggtt cccgtgcgcg
     4621 cggccagttc aagcccgcgg gccggatccc ctgattccag cagcttcctg tattcctcgg
     4681 cgatcctttc cgcgctgacc ttgacgatga acggtgcatt ttcagcgacc gccgcaaggg
     4741 ttgccggctc gatcgaaaaa ccggtctggg ccgcgaaacg gattgcccgc atctggcgca
     4801 ggcggtcttc ccggaaccgc tcggcaggat ccccgatggc cctgattaca ccgttccgga
     4861 tgtcgtcaat cccgccgacc aggtcaacga cgcatccgct taccgggtcg cagactatgc
     4921 cgttcatcgt gaagtcgcgg cgcttcacgt cctcgtgcag atcagtgaag cgaacgtcgt
     4981 ccggcctgcg accgtcggag tggccgatgt cggacctgaa ggtcgcgact tcatagtcga
     5041 agccgtacat cctgacaatc acgaccccga aacgaacccc gaccggaatg gtccggtcga
     5101 atatggcgat gacctgttcc ggcctggcgg atgtcgcgat gtcgaagtcc atgggagtcc
     5161 tgcccatcag caggtcgcga accgaacctc cggccagcca ggcctcgaag cccgcggccg
     5221 acagcatccg ggcaatcctg cgcgcaccct tcaaggcagg attgtcgttc ggaaggtcaa
     5281 gatggggcac cgtgccttcc gcaagccgat ccatcacagg tcctccccgg cgaccacgtt
     5341 gaaacgccac gggcttattg taaccgtgat cgggcccatg tcgggataca gctccccgtc
     5401 gagggtgaaa ttgccggaag tcctgatcat cttcgtgcgc ggtcttgcga cgatcatggg
     5461 atggggaagt tcccacagca ggaatggaac aaggcgcacg acgtcggacg ggtttccggc
     5521 cgagatggtc ttgctgtgga agccttcctc gggcgacgag gcggaaagtg cccacacaag
     5581 cccccgcgcc agcttgacgt caaccgtcga cgcgaccacg ccgaccgtcc ggttacccgc
     5641 cgtggaatcg acgataatcg ggtcggcccg gtgctgaagc cgccatttgt tttcccggaa
     5701 gaacggggtc ttgaatgcgt aacccgcgac gccccttaga aggaacctgg agaatcccga
     5761 gtatccgccc ccgatttcgt cgcacagttc aagggcgttt gcgatcacct gggaaccgaa
     5821 aaccatgccg gtcagggtcc ggccctcttc tgtccgcacc tcaaggagtc caagttccct
     5881 tgtcgagacc ttttccaccg gctggccgcc ccacctggcc tggaaacgcc tgagcgtatc
     5941 aaccgggttg tggttggtgc gcatcgcgcg gctggcgatg ttgtatgtgc ctccgttgag
     6001 cagcagcagt gtcggcctgg cgattccggg gttggcctga agcagcccga tgaccgcgcc
     6061 gatggtgctg tggatggtgc cgtcgccccc gtttatccca agcacgttga ttccgcgatc
     6121 aaccagcaac gttcgcaccg catcgtcgat ttcgcccagg cttgccgtaa ggaccgcgtc
     6181 gccggcctgg gccaggcgct tttcaaggcg cttgtgggtg tatgggaatc tttcgttgtg
     6241 catggccgcg gggttggtta ttacccccag ccgtatcggg ttcatatctg aatgcatata
     6301 ttcaatgtcg gcgggaatgt cagttccgga tgccccagaa caggccgatc gggtcataga
     6361 cggaagatcc gatctggagc ctggcttccc agttggcaag gtcaagctgg ttgccaatgg
     6421 tgtcgaactt catgtcgttg ttcgtgtcga acatttgtgc cggatcgtcc tccggggtcc
     6481 cgagcgtgaa gttttccggc gtcgcgtcaa gcaggttcaa ctgatccctg atgaagccga
     6541 cagcagtgtc cagaagagcc tcgcaggccc cgcccgccag cccggccggc agccaggtgt
     6601 atttctgtgt aagtgtcgcg tcgaagtccg cgcagcacga catggttctt aagcactggc
     6661 gtccggcaag cagggatccg acaagttttt cgtacgtatc gaccgcggga agtccgtcgg
     6721 aaccgtcgcc gaaaacctgc ggaagcagga tcttttccag cgcgaagttg ataagggcgc
     6781 cgtacttcag gttgacggcg tgctggtcga tgtgaaggta ctgaccttcg gtgatcctgc
     6841 cgctgatctc ggcgctgatc gcctttccga tgccgggaat ggacgacatc gagaagtatt
     6901 tggccccgca ttccatgtcg gccgggtcgc agtcctcgcc cagcgaccac ctgaagacta
     6961 cggtgtgcca gttctcggtg cagtcgccct tcgagatgat ggcgccggcg tcgggatcgg
     7021 accagtccat gaccggctcc ttcgtgcagt tcatcgtcga aaggattcgg aacttctgga
     7081 gaatcatgcc gacatcacgc ccggtgaagt agatcttcga gcacaaatcg gggtcgtcct
     7141 cgtaaggaca gttgttcttc agaaggttca cgatgatctg gtcgacgatg ttgatgacga
     7201 atgaaccgat ggttccgtat tcgccgagtt tcggattttt cggatcggca aagatgtagc
     7261 cgcagaaggt ttctcccacg gtgaatccca ggagcgattc gtcgcacatc aggagcagga
     7321 tctggccggt cggggaaacg aaaagcccga tgaggatgtt gataatcttt tcgaccgtcg
     7381 gcggcaggcc ggagatcagg tcgaaattgc tgtcgatgtc ccattcgccg aagatggacg
     7441 gcaccacgtc gtacagcttc aggattaccg tggtcatgtc gccgaattcg acgtggccgc
     7501 cggtgtcgtc gcaggcccat gcccggatgt tccggttgtc ctgtgcctcg tacgcaagtc
     7561 cgacgatggt gtagtcttgg gaaagcacct ggcccgcttc gtttccaagg ccttcaagcc
     7621 tggagaagac cgccagtccg ccgatgatgg cgacgtcggc cttctccacg gtagccgggc
     7681 tgggcaggtt ccgcggatcc atggcggaac aggggtactg gttttccggg gttttcttgt
     7741 aaagacgcac tgtgccgctg ccgttggtca cgttgaagcg gccgtcgtac tgatcgaatc
     7801 tgacccgcag aggcgggatc tttttcggtt cgacgtagat ggtgaccgtg atgcactgat
     7861 cggcggcccc gtcagggcac gcctcgatgt ggcagaagcc cgacgtcaca tcgctctgga
     7921 cgggcatcag ggcaattccg ttcttgtccg tgtagtccat ctggacttga agcgaacaga
     7981 catcctcgcc gtcgatctgc ttgaatgtta caagcctgtc ggcatcgtcc ccgaggcagt
     8041 cgcggtagaa gatctcgaca tttcgaagct ggttgccgct gagcacgatg tcggcgtcca
     8101 gaagcggatc ttccggttcc ccgaccaggc cgaagcccga gacgcacgcg acctcttcat
     8161 cggttcc
//
LOCUS       DAOPPA010000086         8081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_9949_length_8081_cov_3.704710, whole genome shotgun sequence.
ACCESSION   DAOPPA010000086 DAOPPA010000000
VERSION     DAOPPA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9949_length_8081_cov_3.704710"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKH54_04005"
     CDS             <1..515
                     /locus_tag="PKH54_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOC99083.1"
                     /translation="IKPYQKDRVRIWLEMATEEPSTTRNLDKGMQPEQALWAVGSGRF
                     AGKGGGQGVQSRLRYLPEMHNDYIFASFAEERGFLGCVALIILYGVLVMSLLVVAIRA
                     RERFGVLVSVGAVAIIFWQVVFNIGMVLGMFPVVGLTLPLMSYGGSSMISSMFSVGLA
                     LNAGIHRGQV"
     gene            681..2492
                     /gene="dnaK"
                     /locus_tag="PKH54_04010"
     CDS             681..2492
                     /gene="dnaK"
                     /locus_tag="PKH54_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOC99084.1"
                     /translation="MGRVIGIDLGTTNSCVAVMEAGNPVVIPNLEGSRITPSVVAISD
                     EGERLVGAVAKRQAITNASNTVFAIKRMMGRKFDSAEVERIGASYPYEIVKAQNGDAA
                     VSIGGRAYSPPEISAIILKKMKEFAEEYLGETVTEAVITVPAYFDDAQRQATRDAGRI
                     AGLDVKRIINEPTAAALAYGLSKRSSGRVAVYDLGGGTFDISIMELGDGVFQVRTTHG
                     DTFLGGEDVDSRIVDMLADEFERMYGVDLRSDVVALQRLRDASEKAKIDLSAAREVDI
                     NLPFIYADDSGPKHLQIKLTRAQLEALCEELIERTLDHCRQALADAGMKASDLDEVLL
                     VGGMTKMPLVQQRVTSFFGTQASRNVNPDEAVGVGAAIQAGIIQGDVGDVVLLDVVPL
                     SLGIETQGGMFTKLIERNTAIPCSTTEVFTTAEDYQPMVNIHVLQGERPMARDNKSLA
                     RFELIGIPPARRGIPKVEVSFEVDVNGILSVSARDLGTGNVQTVRVRPTSGLSERDIE
                     RIINEAEDSSREDDKRQEVANLKNRADGLMYTTERSLEEFLNYLTDDEVAAINADLEA
                     CRRARGSNDMATIIQAIKNLEKSSYRIAELMYRDSTV"
     gene            2510..>3599
                     /locus_tag="PKH54_04015"
     CDS             2510..>3599
                     /locus_tag="PKH54_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC99085.1"
                     /translation="MYAPFRACLRFSSRISFLAVVLSLGIVLSVNDVVATESDPRTAP
                     DNAPGQSAADCADCVREPPLVAILLPTSGSHSFLGMAVHDELLEKLSPIKGVRVAVFD
                     TAGTAQGARRAAAEAVERQAAMMVGGIGDRETAEISQIATREGVLFLTMGRSRLAAAA
                     TTVFQTVASREAVNRSLLASLAASGKVPECAIVIRRADAFGEAELAAFRGALAGTPIS
                     FIGSRTAGGDSFDEGAFSTEVTSMLSSHRASHACSTVLLHLAIDVPIARRLVDYLRFA
                     GFFEQPGCGVVLSGTSLFNAPGLVAQSGQAFLGLVFVDIDVESSFGGRDDRFRAEASD
                     LSDFAARAATILAEGGAVPPPALMEGAVV"
     assembly_gap    3600..3609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3610..3803
                     /locus_tag="PKH54_04020"
     CDS             <3610..3803
                     /locus_tag="PKH54_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99086.1"
                     /translation="GRTGNLVVRNGRVEGHRLKFCTIGARGIDCLAIEGPSDTREAAP
                     APEARGPDAPVPTESTLRK"
     gene            3811..4797
                     /gene="thiL"
                     /locus_tag="PKH54_04025"
     CDS             3811..4797
                     /gene="thiL"
                     /locus_tag="PKH54_04025"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOC99087.1"
                     /translation="MDEFELIDRLVRLFDESGASLPDGVLVVPNGDDAAVVRPSSGLS
                     TLTTDALVDGVHFALDLCSLQDAGFRSIAVNLSDLAAMGATPTGLLVSLILPPSLADC
                     DVLEIGRGIACASARFDVPVIGGNITRTSGPTVISITACGDQRGPCGPRSGARIGDGL
                     WVSGHPGDGAIGLRLLQSHPLIAERFPRLVSAWRRPQPRLDLIPLFHRGLVDAAIDIS
                     DGLLADLGHICKASGILAAVDKGAIPVSQDAIQAARMAGIPDLDRLVLTGGDDYQLLI
                     AAPPSSEPFMIDAGLARIGTFEPGAGVEIVESGVRNRFVGNRGWLHRQDRDS"
     gene            complement(4794..5126)
                     /gene="trxA"
                     /locus_tag="PKH54_04030"
     CDS             complement(4794..5126)
                     /gene="trxA"
                     /locus_tag="PKH54_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC99088.1"
                     /translation="MANSKNVLEFTDSSFKAEVMQSDKPVLVDFWAPWCGPCRAIAPI
                     IEEIADAYAGRVKVGKVNVDENVGVANGFAISAIPTLLFIKDGKVADQLVGAADRSKI
                     AARLDKIL"
     gene            complement(5126..5473)
                     /locus_tag="PKH54_04035"
     CDS             complement(5126..5473)
                     /locus_tag="PKH54_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOC99089.1"
                     /translation="MDDCSSSCEEKLNASLGSIDFSTFVISLGTSALYHLGANVPEAP
                     GATTVNLPMARNIIDIIVMLKEKTAGNLTDDEVRLLEALLFDLRLKYVETCGKSSASS
                     PETSTSQEPNKEQ"
     gene            complement(5516..5686)
                     /locus_tag="PKH54_04040"
     CDS             complement(5516..5686)
                     /locus_tag="PKH54_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99090.1"
                     /translation="MDKEILFDTRLVNRWISRKVISTGDYEKWLKELPDVSANAENIE
                     LSSRDHLESSGS"
     gene            5921..6586
                     /locus_tag="PKH54_04045"
     CDS             5921..6586
                     /locus_tag="PKH54_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99091.1"
                     /translation="MSRQTEDTMKPAGLESPPSSGTLASARKVVVPVPEPVAPDGPVV
                     LPLIPDENLGLLPSSYDMDRLYAVPRDPERVFATFGISRATMARAGSADIAIRFVFED
                     GVNSSDFIDVIPPGSSVYYCAVPPGRLWFTVELGFLDRGRFEGFLSFGPVPTAVAFVR
                     PGTPEFVRFDPDAPPGPGWLAAGMTAVPAPDLPGPAFKAANVEAGLVQRNWIGYMPVS
                     GRK"
     gene            6588..>7525
                     /locus_tag="PKH54_04050"
     CDS             6588..>7525
                     /locus_tag="PKH54_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOC99092.1"
                     /translation="MPKGFVSLVLHAHLPFVKHPEYAEFLEEDWFFEAAVETYLPLLD
                     MIERLNREGVPFRLNMVVSPTLAAMMDDRHHQERLKASIEKTMVLAGNEARRLKDDPE
                     FGPVARFYQDRLGYLHHKLTDVYRGNLINGLRDAMSSGSLELMTCAATHGLLPLLRVQ
                     PQCVRAQVEIGASEFQRHFGVRPRGIWLPECGYYEGVEEYLADCGIRFFIVDTHCLTY
                     ASPSPAFGVYAPLMTKSGVAAFGRDIESSRQVWSAREGYPGDDAYREFYRDVGYDRPF
                     DEVREFVQPNGLRKQTGLKFHRVTGDVDLSQKLAYNR"
     assembly_gap    7526..7535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7553..>8081
                     /locus_tag="PKH54_04055"
     CDS             7553..>8081
                     /locus_tag="PKH54_04055"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOC99093.1"
                     /translation="MRRVDAHAGNFMFNRGRQVEHLAGEFGRPPIVVAPYDAELFGHW
                     WFEGPDFLEVLFRKTAAEQDTFRFVSLAEYLSFNQVQQIGTPATGSWGDRGYFEVWIN
                     RENDYIPRHLFECAQEMIALADQYSCPTGLEARALNQCARELLLAQSSDWPFIITMGT
                     MTEYAHRRVREHVLRF"
BASE COUNT         1452 a         2381 c         2467 g         1761 t
ORIGIN      
        1 tcatcaagcc gtaccagaag gatcgcgtca ggatctggct tgaaatggca accgaggagc
       61 catcgaccac caggaacctg gacaagggca tgcagcccga acaggctctg tgggcggtcg
      121 gttcgggcag gttcgccggc aagggcggcg gccagggcgt gcagtccagg ctgcgatacc
      181 ttccggaaat gcacaacgac tacatcttcg cgtctttcgc ggaagagcgg ggatttctgg
      241 gatgcgtcgc cctgatcatc ctttacggcg ttctggtcat gtcgctgctt gtagtcgcca
      301 tcagggcccg cgaacgcttc ggggtgctcg tcagcgtggg ggccgtcgcc atcatcttct
      361 ggcaggtggt cttcaatatc gggatggttc ttggcatgtt ccccgtcgtc ggcttgaccc
      421 ttcccctgat gtcgtacggc ggttcgtcga tgatttcctc catgttttcc gtcgggctcg
      481 cccttaatgc cggcatccac cgcgggcagg tttagggcgc ggcgccagat tccgtcttcg
      541 tgttttcgat tcacggaaga tcctgaataa cagtgtaaaa acatagttaa gatttgagaa
      601 atcccgttcg tggtggtagt atttccggca attgtgtgga cagcgttcct ttcgcggtgt
      661 cgttcggagg gtatttgctt atgggcaggg taatcgggat tgaccttgga accacgaaca
      721 gttgcgtggc cgtgatggag gccgggaacc cggttgtcat tccaaacctg gaaggcagcc
      781 gcatcacgcc gtccgtcgtt gccatcagtg acgaaggtga gcgtctggtc ggtgccgtgg
      841 ccaagcgtca ggccattacc aatgcatcca ataccgtttt tgccatcaag cgcatgatgg
      901 ggcgcaagtt cgattccgct gaagttgaac gaatcggtgc ttcgtatccg tacgagatag
      961 tcaaggcgca gaacggcgac gccgcggtca gcatcggtgg acgtgcgtat tccccccccg
     1021 agatttctgc aatcatcctg aagaaaatga aggagttcgc cgaggagtac cttggtgaga
     1081 ccgtcaccga ggccgtcatc acggtcccgg cttatttcga tgatgcccag aggcaggcca
     1141 cccgcgacgc gggaaggatt gccggcctgg acgtgaagcg aatcatcaac gaaccgacgg
     1201 ccgcggccct tgcgtacggc ctgtccaagc gttccagcgg ccgggtcgcc gtctatgacc
     1261 ttggtggtgg aacgttcgat atctcgatca tggaacttgg cgatggcgtc ttccaggtca
     1321 ggacgaccca cggcgacacc ttcctgggtg gcgaggatgt cgattccagg atcgttgaca
     1381 tgctcgctga cgagttcgag cgcatgtacg gcgtggatct tcgatcggac gtcgtggcgc
     1441 tgcagcgtct tcgcgatgcg tctgaaaagg ccaagattga cctttccgcg gcccgcgagg
     1501 tcgatatcaa ccttccgttc atatacgccg acgacagcgg cccgaagcac cttcagatca
     1561 agttgaccag ggcccagctc gaggcccttt gcgaagagct tatcgaaagg acgctcgacc
     1621 attgccgaca ggctctggca gacgccggca tgaaggcttc cgatctggat gaagtgcttc
     1681 tggtgggagg catgacgaaa atgccgctgg tgcagcagcg ggtcaccagc ttcttcggga
     1741 cccaggcaag tcgaaatgtc aatccggacg aagctgtcgg cgtcggtgcg gccatccagg
     1801 ccggtatcat ccagggcgac gtcggcgacg tcgtgctgct tgacgtcgtt cctctgtcgc
     1861 tcggcatcga gacccagggc ggcatgttca ccaagctgat tgaacgcaac acggcgatcc
     1921 cctgttcaac gaccgaggtc tttaccacgg ccgaggatta ccagccgatg gtcaacatcc
     1981 acgttctgca gggcgagcgt ccgatggccc gcgacaacaa gtcgcttgcc cgcttcgaac
     2041 tgatcggcat tccgcctgcc cgaaggggca tcccgaaggt cgaggtgtcg ttcgaggtcg
     2101 atgtcaacgg cattctcagc gtttccgcgc gcgacctggg gaccggaaac gtccagacgg
     2161 tgcgcgtccg tccgacatcg ggcctgtcgg agcgcgatat cgaaaggatc atcaacgagg
     2221 ccgaggacag ctcgcgcgag gacgacaagc gccaggaagt cgccaacctg aagaacaggg
     2281 cggatggcct gatgtacacg accgaacgct ccctcgagga attcctgaac tacctgaccg
     2341 acgacgaagt cgcggccatc aatgcggatc tcgaggcgtg caggcgcgcc cggggaagca
     2401 atgacatggc gaccatcatc caggccatca agaacctgga aaagtcgtcc tacaggattg
     2461 ccgagctgat gtaccgcgat tcaaccgtct gaccaattcg gggggtgcca tgtacgcccc
     2521 tttccgggct tgcctcagat tctccagtcg catttcgttc ctggcggtcg ttctgtccct
     2581 gggaatcgtc ctttcggtca acgacgtcgt cgcgaccgaa agcgatccgc gtacggcgcc
     2641 ggataatgcc cccgggcagt ccgccgccga ttgcgccgat tgcgtccggg agccgccgct
     2701 ggtcgccatc ctgcttccga ccagcggcag ccattcattt cttggaatgg cagtacacga
     2761 tgaacttctt gaaaagcttt cgcccataaa gggtgtccgg gtggccgtgt tcgatacggc
     2821 gggaactgcc cagggcgccc gcagggctgc cgccgaggcc gttgaaaggc aggcggccat
     2881 gatggttggc gggattggtg atcgcgaaac cgccgagata tcgcagattg caacccgcga
     2941 aggcgtcctg tttcttacaa tgggccggtc gagacttgcc gccgcggcga cgacggtctt
     3001 tcagacagtc gccagtcgtg aagccgtcaa tcgcagcctt cttgcttctc tggcggcttc
     3061 gggaaaggtt ccggaatgcg cgattgtgat ccgtcgcgcc gatgccttcg gggaggccga
     3121 gcttgccgcc ttcaggggag ccctggccgg gacccccatc tcgttcatag gttccagaac
     3181 cgccggcggg gattcctttg acgagggcgc gttttcgacc gaagtgacgt caatgctttc
     3241 ttcccatcgc gccagccacg cctgttcgac agtccttctg caccttgcca tcgatgtccc
     3301 catcgcccgc aggcttgtcg attacctgcg gtttgcggga tttttcgagc agcccgggtg
     3361 cggcgtggta ctgtcgggca catcgctttt caatgcgccg ggactcgtcg cgcaaagcgg
     3421 ccaggccttt ttgggccttg tttttgtcga tattgacgtg gagtcttcgt ttggcggtcg
     3481 tgacgatcgc ttcagggccg aagcgtcgga tctttcagat tttgccgcca gggccgccac
     3541 gatccttgcg gaaggcgggg ccgtcccgcc gcccgccctc atggaagggg ccgtcgtttn
     3601 nnnnnnnnnc gggaagaacc ggaaacctgg tggtcaggaa cggccgcgtg gagggccacc
     3661 ggctgaagtt ctgcacgatt ggggcccgtg gcatcgactg tctggcaatc gaggggccat
     3721 ccgacacgcg ggaagccgcc ccagcccccg aagcccgagg cccggacgcc ccggttccca
     3781 cggaaagcac gcttcggaag tgagacgcac atggacgaat tcgagctcat tgaccgtctt
     3841 gtccggctct ttgacgaatc aggcgcgtcc ttgccggacg gcgttctcgt ggtgcccaac
     3901 ggcgatgacg ccgcggtggt caggccttcg tccggccttt cgactctgac tacagacgca
     3961 cttgtcgatg gcgttcattt tgcgcttgat ctctgctccc tgcaggacgc cgggttccgg
     4021 tcgattgcgg tcaacctgtc tgatctggcg gcaatgggcg ccacgccgac aggccttctg
     4081 gtttcgctca tccttccccc gtcgcttgcc gattgcgacg tgctcgagat cggccgtgga
     4141 attgcctgcg cgtcggcgcg gttcgacgtt cccgtgattg ggggcaacat caccaggaca
     4201 tctggtccga cggtgatttc cattaccgcc tgcggcgatc agcgcggacc ttgcggcccc
     4261 agatcggggg cacggatcgg tgacggcctc tgggtcagcg gtcatcccgg cgacggcgca
     4321 atcggccttc ggctgcttca gtcccatccg ctgattgccg aaaggtttcc gcgtcttgtt
     4381 tctgcctgga ggcgtccgca acccagactg gatttgatac cgctttttca tcgcgggctg
     4441 gtcgatgccg ccattgatat ttccgacgga ctgcttgccg atcttggcca catctgcaag
     4501 gcatcgggga ttctggccgc ggttgacaag ggcgccatcc ccgtcagcca ggacgccatc
     4561 caggccgccc ggatggccgg gatcccggat cttgaccgcc ttgtcctgac cggcggggac
     4621 gactaccagc ttcttatcgc cgcaccgcct tcatcggaac cattcatgat tgatgccgga
     4681 cttgcaagaa tcggcacctt tgaacctgga gccggtgtcg agattgtcga atcgggtgtc
     4741 aggaaccgtt ttgtgggaaa caggggctgg ctgcacaggc aggatcggga ctcctagaga
     4801 atcttgtcca ggcgggcggc gatcttcgat cggtcggcgg ccccgacgag ctggtcggcg
     4861 acctttccat ccttgatgaa aagcagcgtc ggaatggctg aaatcgcgaa tccgttggca
     4921 acaccgacgt tttcatcaac gttgaccttt cccaccttga ccctgccggc gtaggcgtcg
     4981 gcaatctcct cgatgatcgg ggcaatcgcg cggcacggtc cgcaccaggg tgcccagaaa
     5041 tcgaccagga caggcttatc ggactgcatc acctcggctt tgaaactgga atccgtgaac
     5101 tcgaggacgt tttttgaatt cgccattatt gctccttatt tggctcctga ctggtagatg
     5161 tttccggact tgatgcggac gacttgccgc aggtttcaac gtatttcagg cgaaggtcga
     5221 aaagaagcgc ctccagaagc ctgacctcgt cgtcggtcag gttgccggcg gtcttttcct
     5281 tcaacatcac gataatgtcg atgatattcc gggccatcgg caggttgacg gtggtcgcgc
     5341 ccggcgcttc cggcacgttg gcgccgagat ggtaaagtgc cgaagtccca agcgaaatca
     5401 cgaacgtcga aaagtcgatc gagccaaggc tcgcgttcag cttttcctcg catgaactgc
     5461 tgcaatcgtc catcggtacc cccttgttcg ccaggcagcg cgctttttgt ggttcctatg
     5521 aacccgagct ttccagatga tccctggaag acagttcgat gttttccgcg ttggccgaaa
     5581 catcgggaag ttccttaagc catttctcgt aatcgccagt ggaaataacc ttcctggaaa
     5641 tccagcggtt tacaaggcgc gtgtcgaaaa gaatttcctt gtccattttg gtttttgttt
     5701 ccggttttta taaggcacca ttgccgcaga ctggaattta attccgcgat ggcaggctgt
     5761 caacccgcat gggacaaacc atcgaaggcg ccgggacatc ggcgtgggct tcggatcggg
     5821 cgggcgccga gcaggccgcg cgaattgact gcgaccggtt ttggaacgat gatacgccgt
     5881 tgtaacggcg tttttttgat ggcgccaatc aaggtgggca atgtccaggc agacagaaga
     5941 taccatgaag ccggccgggc tcgagtcgcc cccgtcgtcc ggaaccctgg cttcggccag
     6001 gaaggtcgtc gtgccggttc cggaaccggt cgcgccggat ggcccggtcg tccttcccct
     6061 gatacccgac gagaaccttg gcctgcttcc atcgtcgtat gacatggaca ggctttacgc
     6121 ggttccacgt gatccggaaa gggtattcgc cacgttcggg atttcgcgcg cgaccatggc
     6181 cagggccggt tcggccgata tcgctattcg attcgtgttt gaagacggcg tgaattcgtc
     6241 ggacttcata gatgtgattc cgcccggttc aagcgtttat tactgcgctg ttccgcccgg
     6301 acgactttgg ttcacggttg aactcgggtt tcttgatcga gggcgtttcg aaggcttcct
     6361 gtcgttcggc ccggttccca ccgcagttgc ctttgtccgt cccggaacgc ccgagtttgt
     6421 acggttcgat ccggatgcgc cgccggggcc gggatggctg gccgcgggca tgaccgcggt
     6481 tcccgcgccg gatcttcccg ggccggcctt caaggcggcc aatgtcgagg cgggacttgt
     6541 tcagcgcaac tggatcggct acatgccggt gagcgggagg aagtgacatg ccgaaaggtt
     6601 tcgtttctct cgtgctccac gcccaccttc ctttcgtgaa gcacccggaa tatgccgaat
     6661 ttcttgagga agactggttt ttcgaggccg ccgtcgaaac ttatctgccg cttctggaca
     6721 tgatcgagcg gctcaatcgc gagggcgttc cgttccgtct taatatggtc gtttccccga
     6781 cgcttgcggc gatgatggac gatcgtcatc atcaggaacg cctgaaggct tccatcgaaa
     6841 agacgatggt tcttgccggc aacgaggcca ggcgccttaa ggacgacccc gagttcggcc
     6901 cggtggccag gttttaccag gatcgccttg gctatctgca tcacaagttg accgacgttt
     6961 accgcggaaa ccttatcaac ggtcttcgcg atgcgatgtc gtccggcagc cttgaactga
     7021 tgacctgtgc ggcgacccat ggactccttc cgctgcttcg ggttcagccc cagtgcgtca
     7081 gggcccaggt cgagatcggc gcgtccgagt tccagcgtca tttcggtgtc cgtccgcgcg
     7141 gcatctggct ccctgaatgc gggtactatg aaggtgtcga ggaatacctt gccgactgcg
     7201 gcattcgctt tttcatcgtc gatacccatt gtctgaccta tgccagcccc agccccgcct
     7261 tcggggttta tgcgccgtta atgacgaaat ccggcgtggc cgccttcggt cgggacatcg
     7321 agtcgtcgcg gcaggtctgg agcgcgcggg aaggctatcc cggagatgat gcttaccgcg
     7381 aattctatcg tgacgtcggt tatgacaggc cgtttgacga ggttcgcgaa ttcgtccagc
     7441 cgaacgggct gcgcaagcag accgggctga aatttcaccg ggttaccggc gatgtcgatc
     7501 tttcgcagaa gctggcatac aaccgnnnnn nnnnnacaac cggcaggccg cgatgcgtcg
     7561 cgtcgatgcc catgccggca atttcatgtt caaccgcggt cgccaggttg aacaccttgc
     7621 cggtgagttc gggcgtccgc cgattgtcgt cgcgccctac gacgccgaac tcttcgggca
     7681 ctggtggttc gaggggccgg acttcctgga ggttctgttc cgcaagacgg cggccgaaca
     7741 ggacaccttc cggttcgtct cgctggccga gtatctgtcc tttaaccagg ttcagcagat
     7801 tggcacgccc gcgaccggtt cgtggggtga tcgcggatat ttcgaggtct ggataaatcg
     7861 tgaaaacgat tacattccgc gtcacctttt cgagtgcgcc caggaaatga ttgccctggc
     7921 ggatcagtac tcctgtccaa caggactcga ggccagggcg ttgaatcaat gcgcgcgcga
     7981 actgttgctg gcccagtctt ccgactggcc gttcatcata accatgggaa ccatgacgga
     8041 gtatgcccat cgccgcgtgc gggagcacgt ccttcgcttt t
//
LOCUS       DAOPPA010000087         8031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10045_length_8031_cov_4.121615, whole genome shotgun sequence.
ACCESSION   DAOPPA010000087 DAOPPA010000000
VERSION     DAOPPA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_8031_cov_4.121615"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKH54_04060"
     CDS             complement(<1..442)
                     /locus_tag="PKH54_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99094.1"
                     /translation="MMLSLVTLLVPAAAWAGNETALTGLPGWYSRVPTGALNTHMYFA
                     VDALDSSNLYIGGMNIASGSMQDIFLKTKGLAFISYDAGQIIRDIANVIGAGMAGSVL
                     NPDESSMCTMSDLESWQTEDDTGHKTRYTWAACGNYIGLARQTGK"
     gene            688..3111
                     /gene="leuS"
                     /locus_tag="PKH54_04065"
     CDS             688..3111
                     /gene="leuS"
                     /locus_tag="PKH54_04065"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOC99095.1"
                     /translation="MAPEDSVYDFAEVEARWQKFWLDNRTFRADDDSDRPKFYILDMF
                     PYPSGAGLHVGHPEGYTATDILARYKRMRGFEVLHPMGWDSFGLPAEQYALKTGIHPE
                     ITTKKNIDNFRRQIRMLGLGYDWEREIATSDSSYYRWTQWIFLKLFEHGLAYQTEIPV
                     NFCPALGTVLADEEVIDGRSERGGHPVERRPMRQWMLKITAYADKLLDGLDDLDWPDS
                     IKEMQRNWIGRSEGAEVDFTVDGTGDVFRIFTTRVDTLFGVTFMVLAPEHPLVQKITT
                     EDRVKSVGEYVAKARSMSDIDRTDATREKTGVFTGAYAVNPVSGDKVPIWVADYVLST
                     YGTGAIMAVPGHDDRDYAFARKFGLPVIEVVSPDGQLHGRGEEAFTDYGVAVNSGRYS
                     GMATARMQSAVIADLQARGIGKASVQYRLRDWVFSRQRYWGEPFPLVHMEDGSIRALS
                     ADELPVTLPPVTSYEVAGTGESPLATIEDWVSWTDPRNGARGRRETHTMPQWAGSCWY
                     YLRYLDPHNDTCLVDPEKERKWMPVDLYVGGAEHAVLHLLYARFWHHFLYDIGAVSTK
                     EPFRKLVNQGLILGEDGQKMSKSRGNVVNPDDVVREFGADAMRLYEMFMGPLEVVKPW
                     TTRGVVGTKRFIDRIWRYCSDRLSDETPPRDLQRVLHQTIRKVTEDIEKIRYNTALAQ
                     MMTLQNEAGKAPLKYRQVAEAMVLMLSPFAPHVCEELWERMGHRPSIVDVAWPGFDPD
                     LCVQDTVEVAVQINGKVRGTVVAASDIDQESLKQMALGNENVARHLQGMTVRKVIVVG
                     NRVVNIVAG"
     gene            3154..4032
                     /locus_tag="PKH54_04070"
     CDS             3154..4032
                     /locus_tag="PKH54_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99096.1"
                     /translation="MIVDLHVCIPPSMTDEVIESVLDSCRRAGLDGACLVGIDSVPPV
                     ERIRELAGDLAVFFGVEFSLPRGRLVWIPTDVSHLGLALPFGRSLEEMVAFFKAEGGV
                     MFAAHPYDRSDGPAFSDGIYDLDDISGIEVANAGRDPWKNNMAQDAVVQLKLKGFGGT
                     GRHEAGAAEIGSAATLILDDAQSQADLVAQLGRDDLWAVEFLSDASQFGEDGGDDGHR
                     EHQGGHQCGGQRRQPQAEAQHGHLPGGRPASRTGGHGPQAGDQRRGQQRQPGGGRPQQ
                     RPNRGGQGGRRQAGPK"
     gene            4044..5513
                     /locus_tag="PKH54_04075"
     CDS             4044..5513
                     /locus_tag="PKH54_04075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC99097.1"
                     /translation="MNTLSIVIPVYNERRTLLTLLSRVLSVDLKGMRKELVLVDDCSK
                     DGTTDLVRSLENDVWRERMASMGVPSARLREASIKMLYHQVNRGKGAALRTGFAAVSG
                     DFVIIQDADLEYDPNEYPKLLVPLLDGRADVVYGSRFAGETRRVLLFWHSVGNQFLTF
                     LSNMLTDLNLTDMETCYKVFRADVIKGLNLKSERFGIEPEMTAKLAKMRYRIYEVPIT
                     YSGRSYAEGKKIGMKDGFEALYSIIRFSMFDSDYVKGGVVEETLSKMNALEKFNQYLF
                     DSISKWCGNRILEVGAGTGNITRFLTARGEVTATDYNPDSVDRLKAQFGALEGFRAEA
                     WDVASEPPSWMVEHGFDTIVCLNVLEHVDDHEQALVNMRRILESRQGRLLLLVPSYQG
                     LYSDLDRNLGHFRRYESEELKALLEKTGWKVEESFRLNMAGVLGWFINGPILKRQRLP
                     TAQLGLYELLSPVLLGLEKRIPKRPLGLSIVCVATPSRP"
     gene            5599..6018
                     /locus_tag="PKH54_04080"
     CDS             5599..6018
                     /locus_tag="PKH54_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOC99098.1"
                     /translation="MDGDVSSNDVTIRLRMSAHDAHYGGNLVDGARMLGLFGDVATEL
                     LIRHDGDEGLFRAYDMVEFLAPVYAGDYIEARGRITRVGRTSRVMEFSAFKVIEASRD
                     PETPSAADVLKQPVLVCRATGTCVVPIEFQRFAPKEV"
     gene            6021..6851
                     /locus_tag="PKH54_04085"
     CDS             6021..6851
                     /locus_tag="PKH54_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOC99099.1"
                     /translation="MRQRDPLIITAAICGAETMKDHNPAVPYTARELAREAELCRAEG
                     ASVIHLHVRRPDGSPTQDRRTFGEAIDAIRQAAPDIIIQTSTGGAVGMTVQERTQPLD
                     LKPEFCTLTTGTCNFGDEVFENTFPMIREIAARAVANGVRMEIEAFDAGFVDNALRLH
                     REGVIPAPLHFDFVLGVPGAMTGTEDRLDFMRSIIPADSTWMVAGVGRFELPLASAAI
                     ARGGHVRVGLEDNSYISRGVLAKGSWELVREVRKMAEQAGVTLATPAEARRILSINPA
                     "
     gene            complement(6855..>8031)
                     /locus_tag="PKH54_04090"
     CDS             complement(6855..>8031)
                     /locus_tag="PKH54_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99100.1"
                     /translation="MKYWFAAFLAVFVVMPPSILSAAEPDYSRRPILMIHGYFPVNAG
                     DWVTWGRFKDKLIEDGWPEEYIMNPKFKDPTGCDPDHAREISAWVDELLEKTGWDKVD
                     IIAHSEGGLNTMYYLRHFCGVHKVRNFVGLAIAVHGTRVACVDFVSCGADEMCIPSEK
                     DGGWRQNEPLAAVNACDETLGDIRYTSIWSDYDEIIIPQEGSIIYGARNIELETPMVE
                     HGLIFLVDESLNYIKDALKNKSGYNTDGPGWDCIPQCILPDQAEQADQPEQADVPTIP
                     EAVEVNEGVVEEPSGDVIGDVASDVTSRDVEEADGVIVVPYDAGPKDRGTSDPDDILV
                     TRDLSSPADEGTQVPEQTDSLKGGCSSTGHGNPWSALLVILSAVAMHACGRLVRRRG"
BASE COUNT         1576 a         2248 c         2560 g         1647 t
ORIGIN      
        1 attttcctgt ctgcctggcc aggccgatgt agttaccgca ggcggcccac gtgtatcgcg
       61 tcttgtggcc ggtgtcgtcc tcggtctgcc aggactcgag gtcggacatg gtgcacatgc
      121 tgctttcatc gggattgagc acgcttccgg ccatccctgc gccgatgacg ttcgcgatgt
      181 ccctgatgat ctggccggca tcatagctga tgaaagccag cccctttgtc ttgagaaaga
      241 tgtcctgcat actgcctgac gcgatgttca tgccgccgat gtagagattg ctgctgtcca
      301 gggcatcgac cgcgaagtac atgtgcgtat tcagagcgcc tgtaggaacc ctggaatacc
      361 agccgggaag tccggtcagc gcggtttcgt tccctgccca ggcggcagct ggaacaagga
      421 gagtcacaag ggacagcatc atcagcatct tttttcatgg cacaccacca tcaagcaggg
      481 gccgtccgcc aatctgtcag cccctgacaa ttattgcgcg accctgaaga aaagtatatg
      541 aatccacgcg acaaatccag actgtcaggc tcttccattc caaaatcccg gcggcgccgg
      601 gcggattgga accgccgaac aaggcaaaat cgcctgtttc gtttataatc gccgcgcgat
      661 cggtttttct ttttgaaggt acattccatg gcgcctgaag acagtgttta cgattttgcg
      721 gaagttgagg ccaggtggca gaagttctgg ctggacaatc ggacgtttcg ggccgatgac
      781 gactcggatc ggccgaagtt ttatatcctg gacatgtttc cctatccgtc cggcgccggt
      841 ctgcacgtcg gccatcccga aggatacacg gcgaccgaca tactggcgcg ttacaagagg
      901 atgagggggt tcgaggtcct gcatccgatg ggctgggact ctttcggcct gccggccgag
      961 cagtatgccc ttaaaacggg gattcatccg gaaatcacga cgaagaagaa catcgacaat
     1021 ttcaggcggc agatacggat gctcggcctg ggatacgact gggaaaggga aatcgcgact
     1081 tcggacagtt cctattatcg atggacccag tggattttcc tcaagctctt tgagcacggg
     1141 ctggcttacc agaccgagat ccccgtaaat ttctgccccg ccctgggaac ggtcctggct
     1201 gacgaggaag ttattgacgg gaggtctgag cgtggcggcc atccggtcga acgtcgtccg
     1261 atgcggcagt ggatgctgaa gataaccgcc tatgccgaca agctgctcga tgggctggac
     1321 gatcttgact ggccggattc catcaaggag atgcagcgga actggatcgg tcgttcagag
     1381 ggggccgagg tcgatttcac ggtcgatgga accggtgacg tgttcaggat attcacgacg
     1441 cgcgtcgata cccttttcgg cgtgactttc atggtgcttg cgccggaaca tccgctggtg
     1501 cagaagataa cgactgaaga cagggtgaag tcggtcgggg aatatgtggc gaaggcccgt
     1561 tcgatgtcgg atatcgacag aacggatgcg accagggaaa agacgggcgt gtttaccggc
     1621 gcgtatgcgg tcaatccggt cagtggcgac aaggttccga tctgggttgc cgattacgtg
     1681 ctttcgacct atggcaccgg cgccatcatg gcagttccgg gacacgacga ccgggattac
     1741 gcgttcgccc ggaagttcgg ccttccggtc atcgaggtcg tttcgccaga tggccagctt
     1801 cacggacgtg gcgaagaggc attcaccgat tacggcgttg ccgtcaattc cggaaggtat
     1861 tccggcatgg cgacggcccg gatgcagtcg gcggtcatcg ccgatctcca ggcccgcggc
     1921 atcggcaagg cttcggttca gtaccgcctg cgcgactggg tattctcccg tcagcgttac
     1981 tggggcgagc ctttcccgct ggtacacatg gaagacggct ccatcagggc gctttccgcc
     2041 gacgaacttc cggtaacgct gccgccggtt acctcttacg aggtcgccgg aaccggggaa
     2101 tcgccgctgg cgaccatcga ggactgggtg tcctggacgg atccccggaa cggcgccagg
     2161 ggccgccgcg agacccacac catgccgcag tgggccggtt cgtgctggta ttaccttcgt
     2221 taccttgatc cgcacaacga cacgtgcctg gtcgatccgg aaaaggaaag gaagtggatg
     2281 ccggtcgatc tgtacgtggg cggcgccgag cacgcggtgc tccacctgct gtacgcaagg
     2341 ttctggcatc acttcctgta cgacatcggg gcggtcagca cgaaggagcc cttcaggaag
     2401 ctggtcaacc aggggctgat cctgggcgag gacggccaga agatgtcgaa gtcgcgcggc
     2461 aacgtggtca atcccgacga tgtggtgcgc gagttcggcg cggacgcgat gcgcctgtac
     2521 gagatgttca tgggtcctct cgaggtggtc aagccgtgga ctacccgggg cgtcgtcggg
     2581 acaaagcggt tcatcgaccg aatctggcgt tattgttccg acaggttgtc ggacgaaacg
     2641 ccgcccaggg acttgcagag ggttctgcac cagaccatcc ggaaggtgac cgaggacatc
     2701 gagaagatta ggtacaacac cgcgctggcc cagatgatga ccctgcagaa cgaagcgggc
     2761 aaggcgcccc tgaagtatcg gcaggtggcc gaggcgatgg ttctgatgct gtccccgttc
     2821 gcgccgcacg tgtgcgagga actgtgggaa aggatgggac atcggccgtc catcgttgac
     2881 gttgcgtggc ccggattcga tcccgatctt tgcgtgcagg atacggttga agtggccgtg
     2941 cagataaacg gaaaggttcg cggaacggtc gttgcggcaa gcgacataga ccaggaaagc
     3001 ctgaagcaga tggccctggg caacgaaaac gtggccaggc atcttcaggg aatgacggtg
     3061 aggaaggtta tcgtcgtcgg caaccgtgtg gtcaacattg ttgccggttg acgcgattgt
     3121 tcagcgcatt tcgcgctgtc cgggggctta tcgatgattg ttgacctgca tgtgtgcatt
     3181 ccgccgtcga tgacggacga agtgattgaa agcgttcttg attcatgccg gcgtgccggc
     3241 ctcgacggcg cctgcctggt cgggatcgac tcggtgccgc cagtcgaacg catcagggag
     3301 cttgccggtg acctggcggt ctttttcggg gtcgaattct cgcttccccg tggaaggctc
     3361 gtctggattc caaccgatgt ttcccacctt ggccttgccc ttccattcgg gcggagcctg
     3421 gaggagatgg ttgccttttt caaggccgaa ggcggcgtga tgtttgccgc gcatccatac
     3481 gatcgcagcg acggccccgc attttccgat gggatatacg accttgacga catcagcgga
     3541 atcgaggtgg ccaacgccgg gcgcgatccc tggaagaaca acatggccca ggacgcggtc
     3601 gttcagttga agctgaaggg gttcggcggc acggggcggc acgaggccgg cgcggctgag
     3661 atcggctcgg cggcgacgct gatacttgat gacgcgcagt cacaggcgga tctggtcgcc
     3721 cagcttggca gggatgacct gtgggcggtc gagttccttt cggatgcctc gcagttcggc
     3781 gaggatggcg gcgatgacgg tcaccgggag catcagggcg gccatcagtg tggcggccag
     3841 aggcggcagc ctcaggcaga ggcccagcac ggacatttgc ccggcggtcg gcccgcttca
     3901 agaacggggg ggcatggtcc gcaggcaggc gatcagagaa ggggtcagca gaggcaaccg
     3961 ggcggcggca gacctcagca gcggcccaac aggggggggc agggcggccg ccgtcaggcg
     4021 ggcccgaagt agaagggggg agtatgaaca cgctttcgat agtcattccg gtctataacg
     4081 agcgcaggac tcttctgacc ctgctttcca gggttctttc ggtcgatctg aagggcatgc
     4141 gcaaggaact ggtgcttgtc gatgactgtt cgaaggacgg aacaaccgat ctcgtaagaa
     4201 gtcttgaaaa cgatgtctgg cgggaaagga tggcttccat gggggttccg tcggcccggt
     4261 tgcgcgaagc gtcgatcaag atgctttacc accaggtcaa tcggggcaag ggtgccgccc
     4321 tcaggacggg gttcgcggcc gtgtcggggg acttcgtcat catccaggac gccgatctgg
     4381 aatatgaccc gaacgagtat ccgaagcttc tggtaccgct tctggacggt cgtgccgatg
     4441 tcgtctatgg gtcgcgcttt gcgggcgaga ccaggcgggt actgctcttc tggcattcgg
     4501 tcggcaacca gttcctgact ttcctttcaa acatgctgac tgacctgaac ctgacggaca
     4561 tggaaacctg ctacaaggtt ttcagggccg acgtgatcaa gggactgaac ctgaagtccg
     4621 agcgcttcgg catcgaaccc gagatgaccg cgaagctggc caagatgcgt taccgcattt
     4681 acgaggtgcc catcacttac agcggccgtt cgtacgccga aggcaagaag atcggcatga
     4741 aggacggttt cgaggcgctt tacagcatca tcaggttctc gatgttcgac agcgactatg
     4801 tcaagggcgg cgtcgtcgag gagactctgt ccaagatgaa cgcgctggag aagttcaatc
     4861 agtacctttt tgattcaatt tcgaagtggt gcggtaacag gattctggag gtcggcgccg
     4921 gaaccggcaa tatcacgcgt tttctgacgg ccaggggcga ggttacggcc acggactaca
     4981 atccggacag cgtggatcgt ctgaaggcac agttcggcgc cctcgaggga ttccgggccg
     5041 aggcatggga cgtcgcatcc gagccaccct cgtggatggt cgaacacggg ttcgacacca
     5101 ttgtttgcct gaacgttctc gagcatgtcg atgaccacga gcaggccctt gtcaacatgc
     5161 gcaggattct tgaatcccgt cagggccggc ttctgctgct tgttccttcc taccagggcc
     5221 tgtattcgga tctggacagg aacctgggcc atttccgcag gtatgagtcg gaagaattga
     5281 aggcgctgct tgaaaagacc ggctggaagg ttgaagagag cttccggctg aacatggccg
     5341 gggtgctggg ctggttcatt aacggcccga tcctgaagcg ccagcggctt cccacggccc
     5401 agctcggcct ttacgaactt ctttccccgg tccttctcgg cctggaaaag agaatcccga
     5461 agcgcccgct ggggctttca atcgtctgtg tcgccactcc gtcgcgtccg tgatccgttt
     5521 acccgcaccg gtcaaaccgt cttggcagga ttgtcttttt gcggcattgt tgtccggtct
     5581 tgtaactggt gattcaggat ggatggcgat gtttcttcaa atgatgtgac gattcgtctc
     5641 cgcatgagcg cgcacgacgc gcattacggt ggaaatcttg ttgacggggc gaggatgctg
     5701 gggcttttcg gcgacgtggc gaccgaactc ctgatccgtc acgacggcga tgaaggcctg
     5761 ttccgggcct acgacatggt cgaatttctt gccccggtct atgcagggga ctatatcgag
     5821 gctcgcggtc ggataaccag ggtcgggcgc acgtccaggg tcatggagtt ttcggccttc
     5881 aaggtaatcg aggcatccag ggatccggaa accccgagcg cggccgacgt gctgaagcag
     5941 ccggttctgg tctgccgcgc gaccggaacg tgcgtcgttc cgattgaatt ccagagattc
     6001 gccccgaagg aggtttgaac atgcggcaac gcgatccgtt gataatcacg gcggcaatat
     6061 gcggcgccga gaccatgaag gatcacaatc cggcggtccc atataccgcg cgggaacttg
     6121 cccgcgaggc cgaattgtgt cgcgccgagg gtgcatccgt catacatctg catgttcgaa
     6181 ggccggacgg gtcgccgacc caggatcgcc ggacttttgg cgaggcgatt gatgcaatca
     6241 ggcaggccgc gccggacatc atcatccaga catcgaccgg cggcgctgtc ggcatgaccg
     6301 tgcaggagcg aacccagccg ctggatctga agcccgagtt ctgcaccctg acgaccggga
     6361 cgtgcaattt cggcgacgag gtcttcgaga atacgttccc gatgatccgg gagattgcgg
     6421 ccagggcggt cgcaaacggc gtcaggatgg agatagaggc ctttgacgcg gggtttgtcg
     6481 ataatgccct tcgcctgcat cgcgaaggcg tcattccagc ccccctgcac ttcgatttcg
     6541 tgcttggcgt tccgggtgcg atgaccggaa ccgaggatcg cctggatttc atgcgatcca
     6601 tcatcccggc agattcgacg tggatggtcg ccggcgtcgg tcgtttcgaa ctgccgctgg
     6661 cgtcggccgc aatcgcgcgc ggcggccatg tcagggttgg gcttgaagac aacagctata
     6721 taagcagggg cgtgctggcg aagggttcct gggaacttgt gcgagaggtt cggaaaatgg
     6781 ccgaacaggc tggtgtaacg cttgccacgc cggcagaggc acgccggata ctgtcaatca
     6841 atccagcctg accttcagcc gcgtcgtcga accaggcgac cgcaggcatg catggcgacc
     6901 gccgaaagaa tcacaagcaa ggccgaccac gggttgccat gtcccgtgga agaacagcct
     6961 cctttcaagg agtccgtctg ctcgggcacc tgggtgcctt cgtcggcagg cgaagacaga
     7021 tcccgcgtga ccaggatgtc gtcgggatcc gaggttccgc gatccttcgg gccggcatcg
     7081 tacggaacaa cgatgacgcc gtcggcctct tccacatccc tggatgtcac gtccgaggcg
     7141 acatcgccaa tcacgtcgcc ggacggttcc tcaaccacgc cttcattaac ttcaaccgct
     7201 tcggggatcg tcggaacgtc cgcctgttcc ggctgatcgg cctgctccgc ctgatccgga
     7261 aggatgcact gcgggatgca gtcccatcca ggtccgtcgg tgttgtatcc gctcttgttc
     7321 ttcagggcgt ccttgatgta gttcagggac tcgtcaacca ggaagataag tccgtgctcg
     7381 accataggcg tttcaagttc aatgttccga gcgccgtaaa tgatggagcc ttcctgcggg
     7441 atgatgattt cgtcgtaatc gctccagatc gatgtgtaac ggatgtcccc aagcgtttcg
     7501 tcgcaggcgt tgactgcggc caggggttcg ttctgtctcc agccgccgtc cttttcggac
     7561 ggaatgcaca tctcgtcggc gccgcacgaa acgaaatcga cacaggccac gcgcgtgccg
     7621 tgaaccgcga tggcgaggcc cacaaaattg cgaaccttgt gcactccgca gaagtgccga
     7681 aggtagtaca tggtgttcag gccgccctcg gaatgggcga tgatatcgac cttgtcccac
     7741 ccggtctttt ccagaagttc atcgacccat gccgatattt ccctcgcgtg gtccggatcg
     7801 cagccggtgg ggtctttgaa cttgggattc atgatgtact cttcgggcca gccatcctcg
     7861 atcagtttgt ccttgaacct gccccaggtg acccagtctc cggcgttgac cgggaaataa
     7921 ccgtggatca tcaggatggg ccttcgggaa tagtcgggct cggccgccga cagaatggac
     7981 gggggcatga ccacgaacac agccagaaaa gccgcgaacc agtatttcat c
//
LOCUS       DAOPPA010000088         7964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10153_length_7964_cov_4.106840, whole genome shotgun sequence.
ACCESSION   DAOPPA010000088 DAOPPA010000000
VERSION     DAOPPA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10153_length_7964_cov_4.106840"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PKH54_04095"
     CDS             <1..1159
                     /locus_tag="PKH54_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HOC99101.1"
                     /translation="EGDAAAPADPMPVPECYCTEEWAPVCGANGVTYSNYCFAKCSGV
                     EVIYEGECQGGQGIGYCVADQQPGCYSDYDCPDGYYCQMTDWCAGVDYDQDGVIDPTW
                     CMGQCIPKEIQSCESMGGFCIGLESNHGCPEGHEWITDGIYEDGSSFCGKGMACCVPT
                     QQPQECSSDEDCYNMFGDSSDPATGMPVEWACVDGFCQARQECGQMQCTSDADCGDGM
                     FCTVTSWCDPAGWCCEMTMCVPIEEPPYCTSARECADGEVCINGVCVYEQPACSSDSD
                     CADGLVCIDGLCQFKGDDCARDDSGMCMCGGFAGFACPAGMVCQFDDPNCSPDAGGAD
                     CMGHCYAVPDECVCDMMYAPVCGEDGRTYSNKCMAACAGVKIVSQGECPRR"
     gene            complement(1301..1927)
                     /locus_tag="PKH54_04100"
     CDS             complement(1301..1927)
                     /locus_tag="PKH54_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOC99102.1"
                     /translation="MKADSLRQLKKEATRKRLIDVSLRLFQEKGFDNTTIDEIVHHAD
                     VSQRTFFRHFPTKEAIVFSEVRGRQARFRQLLEADSETVEPFDRVKHALVKTAAEYDR
                     MSDQLLVEYRMVTSSKYLTTRDIEFDAVLEKTLVNTLLTYRGSRYLSRKEARIAAAAI
                     FGAVRVLMDEWFQKECRHSLKSHARLCLSIVDMLAQGFNARGAGPQNR"
     gene            2194..3291
                     /locus_tag="PKH54_04105"
     CDS             2194..3291
                     /locus_tag="PKH54_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99103.1"
                     /translation="MRMLQKFAGVVGMVAVFGLVSCGSDNDIVPVGQVGESASLAITA
                     QDGGVVEVASKAAGIAIPAGALAADTTISINTLDSVGMADEDRLGSFVFDFGPDGTTF
                     GANVTITLKTGGSTPSGKEAKLAWLDGDKWKEVPGSKEVDGKVVGDVNHFTKFVIYFT
                     DKEVVIENGDEECKDLSFSACGGDPVGEWAIQAYCFDTRRENNMDNPYEEVPDCANGN
                     STFVVDIEWLGTINVNSDGTTATHMGHHAKMIATLNDKCLKAMGQGQVSAAQMCDVLG
                     QQAADDGAEVQYKGGKCTITMDQGEDWDEPTTGTWEKVGNELRMNDDDETVPYCVSGN
                     TLTVQHSEEKDTNDDGQPDVTYKDTMVLKRQ"
     gene            complement(3434..5074)
                     /gene="pgi"
                     /locus_tag="PKH54_04110"
     CDS             complement(3434..5074)
                     /gene="pgi"
                     /locus_tag="PKH54_04110"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOC99104.1"
                     /translation="MIGTQNARLKTIPEWRALQRHFDEIRDVHLRDLFEADPGRADFM
                     TIEDVGIIFDYSKNRATRQTIDLLIHLASARSLQERIHAMFSGQKINTTENRAVLHTA
                     LRAPASERIFVDGENVIPEVHLVLERMADFCVKVRSGQWPGHTGLPIRNVVNIGIGGS
                     DLGPVMAYEALRHYSLRDMTFRFVSNVDGTDFAEAVRDLDPARTMFIVSSKTFTTLET
                     MTNARSAREWLVGALGDPAAVSRHFVAVSTNARAVSEFGIDTANMFGFRDWVGGRFSM
                     DSAIGLSTMLAVGPDRFHQMLGGFRAMDDHFRNAPLERNIPVIMGLLSVWYSDFFGAP
                     ATAILPYDQYMKRFPAYLQQLVMESNGKSVTTDGWPVDYETSSIYWGEPGTNGQHSFY
                     QLLHQGTRLIPVDFIGFANSLNPVGRHHDLLMANMLAQAEALAFGRTPDQVRASGVPE
                     SLVPHRTFAGNRPSSTILLDHLDPAALGSLVALYEHSVFVQGVIWDINPFDQWGVELG
                     KALAEKIIPELESEEESTLCHDASTNALIRKYRKMKVS"
     gene            5187..6836
                     /locus_tag="PKH54_04115"
     CDS             5187..6836
                     /locus_tag="PKH54_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99105.1"
                     /translation="MRKFACLFISVFMVACSQGSGSPDSSAFDVDDAVSVDIADSGLI
                     DSSGPDLVEDRTAIDLEDPDSVDVDDVETPDAEEVDAADAADVMDAGTDDGPQVPWYR
                     QLAPSGQKVREIFGVASHMETSEAGDPETEFEFDTYQSIDGARARRGFRWSNVEPEEG
                     VFLWEKTNGAVDRSVEHGVNLMPVVYYGNDWAESDPDKYGTLDVSKYANYAGAMATQY
                     CDSIKEYELWNEQNITRFWHLPPDPAKYAEMVVAASLKIKAACPDASVVFGGMASYDD
                     VDMFDTWNFLRRSLQARPDLCDYFDGVALHPYTFLQFDSPEYDEIDFGFRKPGQTMQT
                     DIARQMLADAGCGEKGIYYTELGWPTYDLSRETVATYAVRSMLLAARDGVTGWYWYTF
                     YDDDPSDSNPIRPHENHFGLFEWAGNAEPWNFTPKPAWSMLVSMLDMVGPFRFAAEVS
                     RIMDLPDDVYVLAFVTDDDILTLAMWDGRDQPDVTYGKSAPGGPGTTFDLLLELPDDT
                     VNTVLFDQAGSQTELPGTGPLIEVTLTPQVQYLYIDRGVTI"
     gene            complement(6891..>7964)
                     /locus_tag="PKH54_04120"
     CDS             complement(6891..>7964)
                     /locus_tag="PKH54_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC99106.1"
                     /translation="IFGLDIESLKSTADRVADEIRSIPGAADVNVEQVAGFSQIDIVP
                     DRQEMARHGLRIGDINGLVGTAVGGTVATEMIDGQASVPVSVRMAQEYRSDPDTIMNL
                     LLRTPAGNDVRLGSVASARGVEGPAQISREGFTRRVVVEVNVSGRDLGGFVSDVRTAL
                     APVVAGLPEGYWLDLGGTWESQQRAMSRLAIVIPLSILLIFMLLVAAQQSVRSAFLVL
                     ANLPFAVVGGLISMAIFKIPFNVPATIGFIALFGVAVQNGMVLLAFERQLRDSGQTPA
                     QAMKAACLGRVRPLLMTALTTSFGLIPMIWASGPGADVERPLAIVMNGGVITSTILTL
                     FILPSIHVMVMERKSVPAKITEK"
BASE COUNT         1623 a         2250 c         2444 g         1647 t
ORIGIN      
        1 cgaaggcgat gccgcggcac cggccgatcc gatgccggtt ccggaatgct actgcaccga
       61 ggaatgggcc cccgtttgcg gagccaacgg cgtcacgtat tcaaactact gcttcgcgaa
      121 gtgctccggc gtcgaagtga tctacgaggg cgaatgccag ggtggccagg gtatcggtta
      181 ctgcgtggcc gaccagcagc cgggatgtta ttccgattat gactgcccgg acggatatta
      241 ctgccagatg accgactggt gcgccggcgt ggattacgac caggatggcg tcatcgatcc
      301 gacctggtgc atgggacagt gcatcccgaa ggaaatccag tcctgcgaaa gcatgggcgg
      361 gttctgcatc ggccttgaat ccaatcatgg ctgcccggaa ggccatgaat ggatcacgga
      421 cgggatttac gaggacgggt catctttctg cggcaagggc atggcctgtt gcgtcccgac
      481 ccagcagccc caggaatgct cgtccgacga ggactgctac aacatgttcg gcgattcaag
      541 cgatcccgcg accggaatgc cggttgagtg ggcgtgcgtc gatggtttct gccaggccag
      601 gcaggaatgc ggacagatgc agtgcacatc cgacgcggat tgtggcgacg gcatgttctg
      661 cacggtcacg tcatggtgcg acccggccgg atggtgctgc gaaatgacga tgtgcgtccc
      721 gatcgaggaa cctccctact gcacatctgc ccgtgaatgc gccgacggcg aagtctgcat
      781 caacggcgtg tgcgtgtatg aacagcccgc ttgttcttcc gattccgact gcgccgacgg
      841 tcttgtctgc atcgacggcc tctgccagtt caagggcgat gattgtgcca gggacgacag
      901 cggcatgtgc atgtgcggcg gatttgccgg cttcgcctgc ccggcaggca tggtctgtca
      961 attcgatgat ccgaactgca gccccgatgc cggtggcgcc gactgcatgg gccactgcta
     1021 tgcggttccc gatgaatgcg tctgcgatat gatgtacgcg ccggtctgcg gcgaggatgg
     1081 tcgaacctac agcaacaagt gcatggcggc gtgcgccggc gtcaagatcg tctcccaggg
     1141 cgaatgcccc aggcgctagt cccggccgat tcattccact ctgattcagg cagctttcag
     1201 acgagtcaat ccctagcttc cggtcgtcga cgtctggcca tcggatcatg gccgaaccgt
     1261 ggatcaacgg ttgcggcaga aggcgggaac ggggatggat ttatctgttt tggggtccgg
     1321 cgccgcgcgc gttgaagccc tgggcaagca tatcgacgat ggaaagacaa agcctggcat
     1381 ggctcttcag gctgtgcctg cattccttct ggaaccattc gtccatcagt accctgaccg
     1441 cgccgaaaat ggcggccgcg gcgattcggg cctccttgcg ggaaaggtat ctggatccgc
     1501 ggtacgtaag aagggtgttt accaaagtct tttcaaggac ggcatcgaac tcgatgtccc
     1561 tggtcgtcag gtatttcgag gaagtcacca tcctgtactc gaccagtaac tggtcggaca
     1621 ttcgatcata ctcggccgcg gtcttcacaa gggcgtgctt gacccggtca aacggttcga
     1681 ccgtctcgga atccgcctcg agcaattgcc tgaaccttgc ctgtcgcccg cgtacctctg
     1741 aaaaaacaat ggcttccttg gtcgggaagt gccggaaaaa ggttcgctgg gacacatccg
     1801 cgtggtgaac gatttcgtcg atggtagtat tgtcgaagcc cttttcctgg aacagcctga
     1861 gggatacgtc gatcagccgc ttcctggtgg cttccttttt cagttgtctc aatgagtcgg
     1921 ctttcaagtc aaaatcaccg tctggatggc cactgtcaca tttcagagtg aatcgctgct
     1981 agtctagcaa acgaaattag tctagcaaac gaaatatcca cagtgaagcg tcaatttttg
     2041 ctgcgcgtgg ataatcgaat ggattggaac tgttccggca atgttgttga cggcctgcaa
     2101 gcctggcgtt aaatagcgaa ttacacttgc cggaacggaa ctatttgcct gtggccggct
     2161 gatattatta accagaaact ttggaggttt cagatgcgca tgcttcagaa gtttgccggc
     2221 gtcgtcggca tggtggccgt tttcggcctg gtctcctgcg gttcggacaa cgatatcgtt
     2281 cccgtgggtc aagtcgggga atccgcttct ctggcgatca cggcccagga tggcggcgtg
     2341 gtggaggttg cgtccaaggc ggccggaatc gcgattcctg caggtgcgct ggctgccgac
     2401 acgacgattt caatcaacac gctggacagc gtcggaatgg ccgacgagga caggcttgga
     2461 tcattcgtgt ttgatttcgg gccggatggc acgacctttg gcgcgaacgt cacgatcacc
     2521 ctgaagaccg gcggcagcac gccaagcggc aaggaagcga agctggcctg gctggacggc
     2581 gacaagtgga aggaagtccc cggcagcaag gaagttgacg gcaaggttgt tggcgacgtc
     2641 aatcacttca ccaagttcgt catttacttc accgacaagg aagtcgtcat cgaaaacggc
     2701 gacgaggaat gcaaggatct gtcgttcagt gcctgcgggg gcgatccggt tggcgaatgg
     2761 gcaatccagg catactgctt cgatacccgc cgcgaaaaca acatggacaa tccgtacgag
     2821 gaagtgccag attgcgccaa cggcaattcg actttcgtgg tcgatatcga atggctcggc
     2881 acgatcaacg tgaattcgga tgggaccacg gcgacccaca tggggcatca tgcgaagatg
     2941 attgccacgc tcaatgacaa gtgtctgaag gcgatggggc agggtcaggt ttcggccgcc
     3001 cagatgtgtg atgtgctggg gcagcaggcc gccgacgatg gcgcggaagt ccagtacaag
     3061 ggtggaaagt gcaccattac gatggatcag ggcgaggatt gggatgagcc caccaccggc
     3121 acatgggaaa aggtcggcaa cgaactgcgc atgaacgatg acgacgaaac cgtcccgtac
     3181 tgcgtcagcg gcaatacgtt gacggttcag cacagcgagg agaaggacac caacgacgac
     3241 ggccagccgg acgtgaccta taaggacacc atggttctga agcgccagta gtccgatcaa
     3301 atccctgatt taattcaatt atcttcagtt tttgcaggcc gtcgcctggc gggtggctcc
     3361 acatgccgcg tgcagggggg tgcaacgcca catccgacag tcatgaggtt gccatgcggt
     3421 ggcgccggcg gcctcaggac actttcatct tccgatactt cctgataagc gcgttggtcg
     3481 aggcgtcgtg gcaaagcgtt gattcttctt ccgattcaag ttccgggatg atcttttcgg
     3541 caagtgcctt gcccagctcg acgccccact gatcgaaagg attgatgtcc cagatgaccc
     3601 cctggacgaa gaccgaatgc tcgtaaaggg cgacgagcga cccgagggcg gccgggtcaa
     3661 ggtggtcgag gagaatggtc gatgacgggc ggttcccggc gaacgtcctg tgtggaacaa
     3721 ggctttccgg aacccctgac gcgcgtacct ggtcgggcgt gcgtccgaag gcaagcgcct
     3781 cggcctgtgc caacatgttt gccatgagca ggtcgtgatg ccggccaacc gggttcaggg
     3841 agttggcgaa accgatgaaa tcgactggaa tcagtcgcgt gccctggtgc agaagctgat
     3901 agaacgaatg ctggccgttg gtgcccggtt ccccccagta gatcgacgaa gtctcgtaat
     3961 cgaccggcca gccgtccgtt gtcaccgact tgccgttgct ttccatcacg agttgctgaa
     4021 gataggccgg gaagcgcttc atgtactggt catatggaag gatcgcggtc gccggggcgc
     4081 caaagaagtc cgagtaccag accgacagaa ggcccatgat aaccgggatg tttcgttcaa
     4141 gcggggcgtt gcggaaatga tcgtccatgg cgcggaatcc gccaagcatc tggtggaagc
     4201 gatccggccc gacggccagc atggttgaaa gcccgatggc cgagtccatc gagaacctgc
     4261 cgcccaccca gtcgcggaaa ccgaacatgt ttgctgtgtc gatgccgaac tcggatacgg
     4321 cgcgcgcatt cgtggaaacg gccacgaaat ggcgcgatac cgcggccgga tcgcccagcg
     4381 cgccgacaag ccattcgcgg gcgcttctgg cattggtcat cgtctcaagg gttgtaaagg
     4441 tcttgctgct tacgatgaac atggttcggg ccggatcaag atccctgact gcctcggcga
     4501 agtcggtgcc gtcaacattg gaaacgaacc tgaaagtcat gtcccgcagg gagtagtggc
     4561 ggagggcctc gtacgccatg accggaccaa ggtcggaacc gccgatgccg atgtttacga
     4621 cattgcggat cggaagcccg gtatgtcccg gccactgacc gcttcttacc ttgacgcaga
     4681 agtcggccat tcgttccagg accaggtgaa cttcgggaat cacgttctcg ccgtcaacga
     4741 aaatccgttc ggatgcggga gccctgagtg ccgtgtgcag gacggcgcgg ttctcggtgg
     4801 tgttgatctt ctgtcccgaa aacatcgcgt ggattcgttc ctgcagcgac cttgcgctgg
     4861 ccaggtgaat cagcaggtca atcgtctgtc gagttgcccg gttcttcgaa taatcaaaaa
     4921 tgattccaac gtcttcgatg gtcatgaagt cggcgcgtcc ggggtcggcc tcgaaaaggt
     4981 cgcgaaggtg cacatcccgg atttcatcga agtgacgctg aagtgcgcgc cattcgggaa
     5041 tcgtcttcaa tctggcgttc tgggttccaa tcatgtctgg tcgtcccggc atcaagacgc
     5101 ggggatttta cacccgaagg cacatttttg ttgacggcgc gatgcgtcaa agtgcaaatt
     5161 catttcagtc ttgccgtggt gccaacgtga gaaagtttgc gtgtctgttt atttccgtct
     5221 ttatggtggc ctgttcccag ggttcgggat cacccgactc atcggctttt gacgttgatg
     5281 acgcggtctc ggtcgatatc gccgattccg gccttattga ttcgtccggc ccggatcttg
     5341 tcgaggatcg caccgccatc gatcttgaag atcccgattc ggttgatgtc gatgacgtcg
     5401 agactcccga tgcggaggaa gtcgatgcgg ccgacgccgc cgacgtgatg gatgccggaa
     5461 ccgatgatgg cccgcaggtt ccctggtacc ggcagttggc gccgtcgggc cagaaggttc
     5521 gcgagatctt cggggtcgca tcccacatgg aaacatccga ggccggcgac ccggaaaccg
     5581 agttcgaatt cgacacttat cagtcaatcg acggtgcgcg tgccaggcgc ggcttccggt
     5641 ggagcaacgt cgagccggaa gagggcgtgt tcctgtggga aaagacgaac ggggccgtcg
     5701 atcgctctgt cgagcatggc gtcaacttga tgccggttgt ctattacggg aatgactggg
     5761 ccgagtcgga tccggacaag tacggaaccc tggacgtgtc gaagtacgcc aactacgccg
     5821 gcgcgatggc aacccagtat tgcgattcca tcaaggaata cgagttgtgg aacgaacaga
     5881 acatcaccag gttctggcat cttccgcccg accctgccaa gtacgccgag atggtcgtcg
     5941 cggcctccct gaagattaag gcggcctgcc cggacgcgag tgtcgtgttc ggcggaatgg
     6001 cgtcgtacga cgacgtcgat atgttcgata cgtggaattt ccttcggcgc agccttcagg
     6061 cgcgtcccga cctgtgcgac tacttcgatg gcgtcgcgct tcatccatat acgttccttc
     6121 agttcgattc gcccgaatac gacgagatcg atttcggctt caggaagccc ggccagacca
     6181 tgcagaccga cattgcccgc cagatgctgg ccgatgcggg atgcggcgag aagggcatct
     6241 attacaccga actggggtgg cccacgtacg acctgtccag ggaaactgtc gcgacatatg
     6301 cggtgcgcag catgcttctt gccgcgcgcg acggcgtgac cggatggtac tggtacactt
     6361 tctatgacga cgatccttcc gattcgaacc cgatccgccc gcatgaaaac cacttcgggc
     6421 tgttcgaatg ggcgggaaat gccgagccct ggaatttcac gccgaagcct gcctggtcga
     6481 tgctggtttc catgctggac atggtcgggc ctttccggtt tgcggccgag gtcagccgca
     6541 tcatggatct gcctgatgac gtctatgtcc tggcgttcgt caccgacgac gatatcctga
     6601 cccttgccat gtgggatggt cgcgaccagc cggacgtgac ttatggcaag tcggcccccg
     6661 gcggcccggg aactaccttc gaccttttgc ttgagctgcc ggatgacacg gtcaatacgg
     6721 tgctttttga tcaggccggg tcacagacgg aattgccggg caccgggccc cttatcgaag
     6781 tgacccttac gccgcaggtt cagtatcttt acatcgatcg aggtgtcact atctgacggt
     6841 ctcagttcgg tgaatgttct ttttccccaa gtcgtcaatt ccaggtgctt tcatttttcg
     6901 gtgattttcg ccggcacaga cttcctttcc atcaccatca catggattga cggcagtatg
     6961 aacagcgtaa gaatcgtgga agtgatgaca ccgccgttca tcacgattgc cagcggccgt
     7021 tcaacatcgg cacccggccc ggacgcccaa atcatgggaa taaggccgaa cgaggtcgtc
     7081 agggccgtca tcaggagtgg ccggacgcgt ccaaggcagg ctgccttcat ggcctgggca
     7141 ggcgtctgcc cggaatcgcg cagttgccgt tcgaaggcaa gcaacaccat gccgttttga
     7201 acggcgaccc cgaaaagcgc gatgaaaccg attgttgccg ggacgttgaa tggaattttg
     7261 aaaatcgcca tcgagatgag cccgccgacc acggcgaaag gaaggtttgc aagcacgagg
     7321 aaggcggatc ggacgctttg ctgcgcggcg accagcagca tgaaaatcag caggatcgag
     7381 agcgggatca cgattgccag cctggacatg gcgcgctgct ggctttccca tgttccacca
     7441 agatcgagcc agtatccttc cggcagtccg gccacgaccg gcgcaagggc ggtcctgacg
     7501 tccgacacga agccgcccag atcccgcccg gacacgttca cctcgaccac gacccgacgc
     7561 gtgaaaccct cgcggcttat ctgcgccgga ccttcaacac cgcgcgcgga cgcgacactg
     7621 ccaagcctga cgtcgtttcc ggccggcgtt cgcagcagca ggttcatgat tgtgtcgggg
     7681 tcggaacggt actcctgtgc cattcggacc gaaaccggaa ccgaagcctg gccgtcgatc
     7741 atttcggttg cgaccgtccc ccccaccgcg gttccaacca ggccgttgat gtcaccgatc
     7801 cgcaggccgt gcctggccat ctcctggcgg tccggaacga tgtcgatctg gctgaacccc
     7861 gcgacctgct cgacgttcac gtcggcggcg ccagggattg accggatttc gtcggccacc
     7921 cgatccgccg tggatttcag cgattcgata tcaaggccga aaat
//
LOCUS       DAOPPA010000089         7941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10200_length_7941_cov_3.860385, whole genome shotgun sequence.
ACCESSION   DAOPPA010000089 DAOPPA010000000
VERSION     DAOPPA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10200_length_7941_cov_3.860385"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKH54_04125"
     CDS             <1..292
                     /locus_tag="PKH54_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99107.1"
                     /translation="PLARSEQEMRGCTGHQDCVISCHVDGSCCGGQCGCGNALNRDFA
                     ARLKDWQAARCQGRGQYCPAVACMMRGPFFATCVDGLCRSSEGLGAIIKQTP"
     gene            489..1823
                     /locus_tag="PKH54_04130"
     CDS             489..1823
                     /locus_tag="PKH54_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99108.1"
                     /translation="MEKSFVFLAFLCMAAAFAAGCGGNVVVGDLADVAEDISAIDTVD
                     PADHGGRDVASDHGMTDVDDHDSVSDPGTDPGGDHGIDTVDVDQDAQADEGAPDAADV
                     GDDAEDLPGVDAIDHGQSDQVDPDAADPGREDVADSDAMEPDSVDSDAVADTADVAGD
                     AGTDVLPGPCADGVPEYVGDQGWTGQINVTEAATYCWPFDESLTLRESMARKSRIRLI
                     PGTYPVPTVGEGLSMVLPFCVEFAPGVGGPSHSGQGTVRAGEWGFQYRQPMIFGAAWE
                     FEMSQWASTTTLDGTGSPGSMVLCESSCADWMDRRRFDSCTYDNVDRQIHTIGFEGGQ
                     IVLELRIGMSMASTEPGIFFSATGTLDGKAFDVTDYWKLVYRPAHHHFSRHFAVLLDS
                     LEFGTCSIMVENAVPWPDQPGAELPTVSLADCLLNVTKTKKVTSHTFKRVSP"
     gene            complement(1911..5087)
                     /locus_tag="PKH54_04135"
     CDS             complement(1911..5087)
                     /locus_tag="PKH54_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC99109.1"
                     /translation="MKHNPDFRHFAIAMVTFGILCACGLSGCGTSTVSGDTDVIAGDL
                     PGDVPGDSQMPDSTADDGSGDTGGDTGGDSVEPGPFEWPVQTEDVYITPSADWKNQVE
                     FPDDPFLGGTGYVGYVTPVPRWIKFAVLMKDPGKVYFQDSSKYEFHQQFATERLDPFK
                     GMTTDEFSAVSLYEKDQQVILGAVLYAPGGKPSEVGIQLVRMDPYHPEMVRRVFETVS
                     AAVTMSGETDGILANPLKFFYFPTFEQHPSAMKYRTWLAERNVIVSSVERWATGSQCY
                     SDGWAVGRLAEVAGSELDAAYVSGDLLPTDIVLTDAVPAEVPFVAGIITMTASTPNSH
                     VALLADALDIPFAWLALPADVDHARSLVGKRVAMWTTTEWDSTSCQVHLLDASSLTDD
                     DLTALLGTKSPGKLQFKPKQKKGAYSTPCDEIGPADIVHFGGKAANYGILRDAIPDGS
                     LYAIAFSFDLWDDFMGQPMTGGTLGQAIAARLAAHAQWPADIKTLDADLAAVRGMITD
                     APFPADLQTAVKDALAGFDASTKLRFRSSTNVEDSTEFSGAGLYDSFSGCLGDDLDAD
                     SDGPSHCDGAEAKERGVFRAIKKVYASFFNRNAFLERLRRQVDESKVGMGLLVHYSFP
                     DAQEMANGVAIYKRDTYSRTATLVTQLGALSVANPEGGALPEIASVYAYDQIVTTSVT
                     QESTLVPIGGRVMTWDSDYTALFSMIEKVEKRWEQATGRKQKSLDLEYKRVDPGMLRI
                     KQVREVPDATAEGEIDTYLIGGVAEWCTFQGEYGEVYGTHRVKARATIQVRSGFVKDF
                     ENGAWMESIKLEFAIDGQARTVERQISGQPDVTWTRETDYVTVAFQTGQDSDVVSWRL
                     TVTLPTRRHVQGGPFISTDDLAAWSGLEMKTGAARPWLDWEGNVTGRDTEVASLHACG
                     EGTPVGKDHIRIDRRYIDGDGHQYDIGFWWPPAPTGITAGYTAPLVQWEQTVLTGFTT
                     EPITLTGWWSRSQRPGHYNFSEDYAFEPRLEVGLAPATLAELEEADIAVIYYLATYPQ
                     GDDPAPSGQLCIEHLDKTMTCGSMVYPY"
     gene            complement(5201..>6143)
                     /locus_tag="PKH54_04140"
     CDS             complement(5201..>6143)
                     /locus_tag="PKH54_04140"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HOC99110.1"
                     /translation="PALAVAVLAVTVFAGCEMDPVAQGAVPCEPLTAEEEAVIAFKAT
                     EPRFTGEYVETHDDGVYHCRRCGRPLYESAYKFNSGSGWPAFDRAIEGAVTETPDADG
                     FRTEITCTYCGGHLGHVFRGEGFTETSTRHCVNSISLKFLPDDPGAAQANREFLETRC
                     QFAYFAGGDFRALEYCTQLEPGVITAYSGFMGGSADNPSFAEVQVGDTGHAQTVRVEF
                     DQSMTDYLTLARRFMKLHDPTVELIDPPLGGSQFRSIMFVVNEDQRQQATALLAELAD
                     DEPPISTGINDAGHFWLAPYTYQDFYSDLPVKPACAE"
     assembly_gap    6144..6153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6475..7752)
                     /gene="tyrS"
                     /locus_tag="PKH54_04145"
     CDS             complement(6475..7752)
                     /gene="tyrS"
                     /locus_tag="PKH54_04145"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOC99111.1"
                     /translation="MGRVLDSLKQRGMFSQVTNEAEVAKLLDTPGNAIYVGFDPTADS
                     LHVGHMLPIMGLAMLQRAGHIPIALVGGATGMVGDPSGRSTERNLLTADEVRHNSECI
                     RKQLMHFLDFSGSNAAIMENNADWTEKLTAIEWLRDVGKHFTINYMLAKESVKRRVED
                     RESGVSYTEFSYMILQANDFLQLFRRHNCRLQAGGSDQWGNITAGIDLIRRVTGTEAF
                     GITFPLVTTSSGEKFGKSAGNAVWLDPARTSPYRFYQYWLQTEDADVEKFLGFFSFLP
                     WEEIQAISAEHMAAPERRVGQRRLAEEVTRLVHGEAELQRAMNASKALFGGSLNGLEK
                     RDLDDIFADVPSATMNRADIAAGIGIVDLLVKSGMCASNGEARRMVQGGGIYVNNERV
                     GEPAATFGPDALLADSVIVLRSGKKNYRLVRFG"
BASE COUNT         1508 a         2462 c         2341 g         1620 t
ORIGIN      
        1 cccgttggcc aggtcggaac aggagatgcg tggctgcacc ggtcaccagg actgcgtgat
       61 ttcctgccac gttgacgggt catgctgtgg cggccagtgc gggtgcggca atgcccttaa
      121 ccgggacttc gccgcgcgtc tgaaggattg gcaggccgcc cggtgccagg gccgcgggca
      181 gtactgtccg gcggttgcct gcatgatgcg gggtccgttc ttcgccacgt gcgtcgatgg
      241 cttgtgccgg tcatccgaag gtctgggcgc aatcatcaaa caaaccccct gaattcttac
      301 gtccttgccc ccgcgccctg taagggcgaa tgaaaattcg cccgcccctg gctgatgcga
      361 tccgcccccc gttccgtaag ggcgaatgaa aattcgcccg cctgcgcgaa aattcgcgcg
      421 cgccccgcag gcatttgccg caatccgggt atctgttatt cttcatgcaa cccggtgcag
      481 gtaaatccat ggaaaaatcc ttcgtcttcc tggcgttcct ttgcatggct gccgcttttg
      541 cggccggatg cggcgggaat gttgtcgttg gtgacctcgc agatgtcgcc gaggatattt
      601 cggcaatcga cactgtggat ccggccgacc atggcggccg cgatgtcgcg tcggatcatg
      661 gcatgaccga tgttgacgac cacgattcgg tttccgatcc aggtacggat cccggcggcg
      721 accatggaat cgacacggtg gatgtcgatc aggacgccca ggcagacgaa ggggcgccgg
      781 atgctgctga cgtcggggac gatgccgaag atcttcctgg cgtcgatgcc atcgaccacg
      841 gccagtcgga ccaggtcgat ccggatgccg ccgatcccgg ccgcgaagat gtcgccgact
      901 cggatgccat ggaaccggat tcggtcgatt ccgacgcggt cgccgacacc gccgatgtcg
      961 caggggatgc cggaaccgat gtcctgcccg gaccttgtgc ggacggcgtt cccgagtatg
     1021 tcggtgacca gggctggaca ggtcagatca acgttaccga agccgccacg tactgttggc
     1081 cgttcgatga atcgctgact ttgcgggaat cgatggcaag gaagtcaagg attcggctca
     1141 ttcccggcac ttatcctgtt cccacagttg gcgagggcct ttcgatggtt cttccgttct
     1201 gcgttgaatt cgcccccgga gtcggtggcc cgtcccattc ggggcagggt accgttcgcg
     1261 ccggcgaatg gggctttcaa tacaggcagc cgatgatttt cggtgccgcc tgggaattcg
     1321 agatgtctca atgggcatcg acaacgacgc ttgacggcac tggctcgcct ggatcgatgg
     1381 ttctttgcga gtcgtcctgc gccgactgga tggatcgacg cagattcgat tcctgcacgt
     1441 atgacaatgt tgatcggcag attcacacaa tcgggttcga aggcggtcag attgtcctgg
     1501 aactccgcat cggcatgagc atggcatcca ccgagcccgg aatctttttc tctgcaaccg
     1561 gcacgctcga cgggaaagct tttgacgtaa ccgattactg gaagcttgtt tatcgcccgg
     1621 cacatcacca tttcagtcgg catttcgccg tcttgctgga ttccctggag ttcggcacct
     1681 gctcaatcat ggttgagaac gcggttcctt ggccggatca gccgggtgcc gaacttccaa
     1741 cggtctcgct ggccgattgc ctgttgaatg tgacgaagac aaagaaagtc accagtcaca
     1801 ccttcaagag ggtttccccc tgaccaccca cccacgcgaa aatctgttcc cccccgattt
     1861 gtaagggcga atgaaaattc gcccgcccac cggattcgtc caggcttggt tcagtacgga
     1921 taaaccatgc tgccgcatgt catcgtcttg tccaggtgtt cgatgcaaag ctggcccgat
     1981 ggtgccggat catcgccctg gggatatgtt gcaaggtaat aaatgactgc aatatctgcc
     2041 tcttccagtt cggccagcgt cgcgggcgcc agcccgacct ccaggcgcgg ctcgaacgca
     2101 tagtcctcgc tgaagttgta atgaccaggg cgctggctgc gcgaccacca cccggtcaga
     2161 gtaatcggtt ccgtcgtgaa tccggtcagc acggtctgtt cccactgcac aagcggcgcc
     2221 gtgtatccgg ccgtaatccc ggtcggggcc ggcggccacc agaatccgat gtcatactga
     2281 tgcccatcgc catcgatata ccgccgatca atcctgatgt gatcctttcc gacaggcgtt
     2341 ccttcaccgc acgcgtgcag cgacgccacc tccgtgtcgc gcccggtgac attgccttcc
     2401 cagtccagcc atggccgcgc ggcccccgtc ttcatttcca gccccgacca cgccgcaagg
     2461 tcgtccgtcg aaatgaacgg ccccccctgg acgtgccgcc ttgtcggaag cgtgacagtc
     2521 aatcgccatg aaaccacatc cgaatcctgt cccgtctgga aggccacggt cacatagtcc
     2581 gtttcgcgcg tccatgtcac gtcgggctgg ccggaaatct gtcgctcgac agtccttgcc
     2641 tgcccgtcaa tcgcgaattc cagcttgatc gattccatcc aggcgccgtt ttcaaagtcc
     2701 ttcacgaagc cgctcctgac ctggattgtc gcccgcgcct tgacccggtg ggtgccatag
     2761 acttcaccgt attcgccctg gaaggtgcac cattcggcca cccctccaat caggtacgtg
     2821 tcaatctcgc cctccgccgt cgcatccgga acttcacgca cctgtttgat ccgaagcatc
     2881 ccgggatcca cgcgcttgta ttccaggtca agcgacttct gctttcggcc ggtggcctgt
     2941 tcccagcgct tttcgacctt ttcgatcatc gagaaaaggg ccgtgtaatc cgagtcccat
     3001 gtcatcaccc gaccgccaat cgggacaagg gtcgattcct gtgtcaccga tgtcgtcacg
     3061 atctgatcgt acgcatacac gctggcaatc tctggaagcg cgccgccctc cgggtttgca
     3121 acagataagg cacccaactg ggtaacaagc gtggcagtcc ggctgtaggt gtcacgctta
     3181 tatatggcga ccccgtttgc catttcctgg gcatcgggaa acgagtaatg aaccagcagc
     3241 cccatgccga ccttcgattc atccacctgc ctgcgcagcc gctccaggaa tgcgttgcgg
     3301 ttaaagaaac tggcatagac ctttttgatg gcccgaaaca cgccgcgttc cttcgcttcg
     3361 gcgccgtcgc aatgggacgg gccgtccgaa tcggcatcca ggtcgtcgcc aagacagccg
     3421 ctgaagctgt cgtaaaggcc ggcccccgaa aattcggtcg agtcctcgac gttcgtggaa
     3481 ctgcggaatc gaagcttggt cgatgcatcg aaaccggcca gggcgtcctt gacggccgtc
     3541 tgcaggtcgg cggggaaggg cgcgtcggta atcatgccgc gcaccgcggc aaggtcggca
     3601 tccagcgtct ttatgtcggc cggccactgc gcatgggccg caagcctggc cgcaatcgcc
     3661 tggcccagcg tgccgccggt cattggctgg cccatgaagt cgtcccaaag gtcgaacgaa
     3721 aaggcgattg cgtacaggct gccgtcggga atcgcgtccc gcaggatgcc gtagttggcc
     3781 gcctttccgc cgaaatggac aatgtcggcc ggcccgattt catcgcatgg cgtcgaatat
     3841 gcgcctttct tctgcttcgg cttgaattgc agtttgcctg gcgacttcgt ccccagaagc
     3901 gcggtcaggt catcgtccgt aagggacgaa gcatccagaa ggtgtacctg gcaggaagtg
     3961 gaatcccact cggttgtggt ccacatcgca acacgcttgc cgacaagcga gcgggcgtga
     4021 tcaacatcgg ccggcagggc cagccaggca aaaggaatgt ccagcgcgtc cgccagcagg
     4081 gcgacgtggg aattcggcgt ggaggccgtc atggtgatta tgccggccac gaaaggcacc
     4141 tcggccggaa ccgcgtcggt caggacgatg tcggtcggca acaggtcgcc ggaaacatag
     4201 gcggcgtcca gttcgcttcc ggccacttcc gccaggcgcc ccacggccca tccatcggag
     4261 tagcactggc tgcccgtggc ccatcgttcc accgaagaca cgattacgtt gcgctcggcc
     4321 agccaagtcc tgtacttcat tgcggacgga tgttgctcga acgtcgggaa gtaaaagaac
     4381 ttcaaggggt tggccaagat gccgtccgtc tcgccggaca tcgtcaccgc ggcgctgacc
     4441 gtttcaaaaa cccgtcgaac catttccggg tgatacggat ccatccgcac gagttggatc
     4501 cccacctcgg acggcttgcc gccgggcgcg taaaggactg cccccaggat gacctgctga
     4561 tccttttcat aaaggctgac ggcggaaaac tcgtcggtgg tcatgccctt gaagggatcc
     4621 aggcgctcgg tcgcgaactg ctggtgaaac tcgtacttgc tgctgtcctg aaaatacacc
     4681 ttgccgggat ccttcatcag gaccgcaaac ttgatccacc gcggaaccgg ggtgacatag
     4741 ccaacgtaac cggttccgcc caggaatggg tcgtcgggaa attcgacctg gtttttccag
     4801 tcggcgcttg gggttatata cacatcttcc gtctggaccg gccattcgaa cggtccaggt
     4861 tcgacggaat cgcccccggt gtcgcccccg gtgtcgcctg aaccgtcgtc ggccgttgaa
     4921 tccggcatct gggaatcgcc cggcacgtcg cccggcaaat cgccggcaat gacgtccgta
     4981 tccccgctga cggtggatgt accgcatccc gaaaggccgc atgcgcacag gatcccgaaa
     5041 gtcaccattg caatggcgaa atgccggaag tcagggttgt gtttcatgat tattccgtcc
     5101 caatttgacc gaggatcata cagaccgcaa gtatatcgga cgcgggccag ggacgcacac
     5161 cttccctcgg gatctgggcc atgttccggg cgggacgctg tcactcggcg caggcgggct
     5221 tcacgggaag atccgaatag aagtcctgat aggtatatgg ggcaagccag aagtgaccgg
     5281 cgtcattgat tccggtcgaa atcgggggct catcgtcggc cagttccgca agcagggcgg
     5341 ttgcctgttg acgttgatct tcgttcacca cgaacatgat cgatctgaac tggctgccgc
     5401 caagcggcgg gtcgataagc tcgactgtcg ggtcgtgcag tttcatgaaa cgtcgtgcca
     5461 gggtcaggta atccgtcatg gactggtcaa attcgacgcg gacggtctgg gcgtggccgg
     5521 tatcaccgac ctggacttcc gcaaaggacg ggttatccgc gcttccgccc atgaagcccg
     5581 aatatgccgt gattacccct ggttcaagct gggtgcaata ctccagggcc cggaagtcgc
     5641 cgcccgcgaa gtatgcgaac tggcagcgcg tttccaggaa ttcccggttc gcctgggccg
     5701 cccccgggtc gtccggaagg aacttcaggg aaatcgagtt gacgcagtgg cgcgtgcttg
     5761 tctcggtgaa gccctcgccc cgaaacacat gtcccaggtg gccgccgcag tacgtgcagg
     5821 tgatttccgt gcgaaagccg tccgcatcgg gcgtctcggt gacagcgccc tcgatggccc
     5881 ggtcgaacgc cggccatcca gaacccgaat tgaacttgta agcggattcg tacagcggcc
     5941 gtccgcagcg acggcagtga tagacgccgt cgtcatgggt ctcgacatac tcgccagtga
     6001 acctgggttc tgtcgccttg aacgcgatga cggcttcctc ttcggccgtc aacggttcgc
     6061 acggaacggc gccctgggca accgggtcca tttcgcatcc cgcaaaaacc gtaaccgcaa
     6121 gaaccgcgac agccagagcc ggtnnnnnnn nnncccccgc ccgacgcccc atcggcaatt
     6181 caaccgcatt gcgcgccccc gttccatcgc agaagtataa tatccggaaa tcattattaa
     6241 agtctgcaca ttgccggccc agaacgatcg cggacccgaa tgtgcaccga ggtcacggcg
     6301 aatagtaaca aattccaggt tacaaggctg atgcgctatg gacaggcggg cgagcatcgg
     6361 ttgacatgcc attatggcgg ccctgccaca tggtgatgca attatcgaaa aatgtttgaa
     6421 actttcgaaa aatgaaagtt tcaagcatcc ggccggggaa gggcgcccag gcttctaccc
     6481 gaacctgacc aggcggtagt tcttctttcc gctgcgcagg acgataacgg aatccgcaag
     6541 cagggcgtcc gggccgaagg tggccgcggg ttcgccgacg cgttcgttgt tgacgtagat
     6601 gccgccgccc tgaaccatgc gccgtgcctc gccgtttgat gcgcacattc ccgatttcac
     6661 aagcagatcg acgatgccga tgccggcggc gatatcggcg cggttcatcg tggcggacgg
     6721 cacatccgca aagatgtcgt ccaggtcgcg cttttccaga ccgttcagcg acccgccgaa
     6781 cagtgccttt gatgcattca tcgcgcgctg caactcggcc tcgccgtgaa ccaggcgggt
     6841 gacttcctcg gcaaggcgcc tttgaccgac gcgcctttcc ggcgcggcca tgtgttcggc
     6901 cgaaatcgcc tggatttctt cccatggcag gaagctgaag aatcccagga acttttcgac
     6961 gtcggcgtct tccgtctgaa gccagtactg ataaaagcgg tacgggctgg tgcgcgccgg
     7021 gtcaagccat acggcgttgc ctgccgactt cccgaacttt tcgcccgacg acgtggtgac
     7081 aagcgggaag gtgatgccga acgcctcggt gccggtgacg cggcggatca ggtcgatgcc
     7141 ggccgtgatg ttgccccact ggtcggaacc gccggcctgc agacggcagt tgtgacggcg
     7201 gaacagttga agaaaatcat tggcctgcag aatcatgtac gaaaattcgg tatagctgac
     7261 gccgctttca cggtcttcca cgcgccgctt gaccgattcc ttggccagca tgtaattgat
     7321 cgtgaagtgc ttgccgacgt cgcgcagcca ctcgattgcg gtcagctttt cggtccagtc
     7381 cgcgttgttt tccataattg ccgcgttgct gccggaaaag tccaggaagt gcatcagttg
     7441 cttcctgatg cattccgagt tgtggcggac ttcgtcggct gtcagcaggt tgcgttcggt
     7501 gctgcggccg ctggggtcgc cgaccattcc cgtggcgccg ccgacaaggg cgattggaat
     7561 atgtccggcc cgctgaagca tggccagccc catgatcggc agcatgtggc cgacgtgcag
     7621 gctgtccgcc gtcggatcga acccgacgta aattgcgtta ccgggcgtgt ccagcagctt
     7681 cgccacttcc gcctcgttcg ttacctgcga aaacatcccg cgctgcttca gtgaatcaag
     7741 cactcttccc atcggtgtcg ctccaggtgg ccggaaaatc cgtgcggatt ataaccctat
     7801 cccgtaaggg cgaatgaaaa ttcgcccgcc tgccaggatg aaagctctcc cgcccgcctg
     7861 ccaggatgaa agctctcccg cccgcccgcc cgttagtatg tgaagtcgtc cgcccgctgc
     7921 ggttccgcaa gatccgtaag g
//
LOCUS       DAOPPA010000090         7833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10387_length_7833_cov_3.141553, whole genome shotgun sequence.
ACCESSION   DAOPPA010000090 DAOPPA010000000
VERSION     DAOPPA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10387_length_7833_cov_3.141553"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKH54_04150"
     CDS             <1..811
                     /locus_tag="PKH54_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99112.1"
                     /translation="AARWGFETEIPPESFMHTWRGIATDTSVGGDSVICMGQGNSTSW
                     ISREPDGLVDIFAGSLGGSRGAEDPETVAMDMIARQGGIGDWVRPGTSAVIPLRRPFD
                     GIVAPGFMLLGDSACMANPINGSGMTVAIDAAIIAADTAHDAMMKGRFEASDLWSYPR
                     DFFRANAELAFLDALKSFVFSAPRDPMLDFFRQGGLRGPAFWKMRPEFGPAGFAAKAG
                     AFARTKGAAALLPPMAGTLTRAAIAERHMALYPSSPSGFNQWKAIRRRITG"
     gene            complement(801..2786)
                     /locus_tag="PKH54_04155"
     CDS             complement(801..2786)
                     /locus_tag="PKH54_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99113.1"
                     /translation="MGERLTRQAERAKRAIKSIGRDDRASTSAATADSAGSPEHDIAC
                     WRTRALDCERMLRSINGLIFRLVRDGAGGYEFTFIEGGAIGSAVYSSETWRGRKLSEA
                     APTDLYPTVAAAIEVAFAGGRGLYEDRGFPAGGCSEGVVLPFSRDAEGNVTEVVCTLL
                     DVSARKMAEMESREKQERLEALVSALPDLVFIFDREGTFVGYNIANEEKLLASPGNFL
                     GARLEDILPAEVADVSRRTLNNVVLTGQKGEFHYRLDLDGVEHYFHARMVPFAGDKVM
                     AVVRNVSEIVETIRQDRMKGVQLDIMARVSRLGYWSTNVRQRKIYWSPEAFDLVGLDA
                     VDEVTPEWIGQVLGSNTLEVIKSAILDAVTMRRFRQIEIRRELGGGRIRYLVMSMEPV
                     IMEDGVVEELHGTVQDITALRELEFQMHHLQKIDSLGRVAGAMAHDFNNMLQIINGYG
                     ECLRKFVATDPTASSYLNAMLDASDRTKDLVASILSFSRRTEPVMHEMPLDQFVADTM
                     NMVKPLMGASVRIELQSSGCECGVRLEKSAFTQVLVNLAVNARDAMADGGVLTFRTSC
                     IDDEPERTRIKARWPWLESGQIASIEIRDTGCGIPEENLERIFAPFFSTKAEGAGTGL
                     GLSTASQIVSRHGGALFVKETGSGGTTFQVLLPAFSL"
     gene            complement(3017..4582)
                     /locus_tag="PKH54_04160"
     CDS             complement(3017..4582)
                     /locus_tag="PKH54_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC99114.1"
                     /translation="MKVYEGTVATCDASDSVCRFLVEDGGRIVWTGNELPERFVGAAR
                     EVLPDGHALIPSFADSHIHFASFALFHSGLDVRSARSIPEIQARLKEFATTNRQDIIV
                     GFGASIHSVAERRLVSRAELDAACGDRPVFIVKYDGHACILNSRLIAMLPSHIRGLRG
                     FHPDTGEMNQEAYFACTDFVTGRIPPMQVVRNMISAADSLVSSGICKIDDVSGVGFPK
                     DLDVDLERWVARGTSSGLQMRIFFQTMDIGKVLRRRLPRIGGCFATALDGCFGSMDAA
                     LLQPYRNTDNRGVLFYEPAKVRDFCVRANRAGLQIQVHAIGDAAFEQAVDGIEAALRD
                     TPRPDHRHGIIHACLPTMEGLRRCADLGIHLPLQPAFLEWELEPLEYITDILGDRAML
                     ISPLKTMVEMGIHVSGGSDAPCTLPDAIAGMHAACNHYVPEQSLSVRQALRLFTIEGY
                     RSTFDDVSRGSLEPGKFADMVVLDRNPLDTPPAEVKFMKVRKVILGGLEYRNRDSGPL
                     LTLLKGILSRRPT"
     gene            complement(4608..5765)
                     /gene="pgsW"
                     /locus_tag="PKH54_04165"
     CDS             complement(4608..5765)
                     /gene="pgsW"
                     /locus_tag="PKH54_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOC99115.1"
                     /translation="MKKLYWRPRKVSTFVLVMVALVAVAGVLLVENVTIQERQKNYKL
                     KLDAARLAAQAYDVVKAERVRLKHPVNLEYDPAGTGMLGDAVTSVTTNPGYITSKRTS
                     ANPNFAAVVVQLFKRAGLNRGDVVAIGMTGSFPAMNINVLAAVHVLGLKPIIITSVGS
                     SQWGANFPDFMWPDIERILVARNILPYPSEVMTRGGIDDNALGLEAEAVTLIDDSIVR
                     SGRPALVVSNYKDSFEKRMEIYDRVAGGEDNIRAYINIGGGTVSLGRSVGKKIFTTGL
                     NTEVTTSIESSDDSVMMRFAQKSIPVFHFSQFNAFAERWGLPVNPTSPQKAGTGPIFV
                     TQNYNPWLAGGLLAVLVLLMIAFVRMDWGYRLFKSSHAQPSVNDSVPERMV"
     gene            complement(5762..6217)
                     /gene="pgsC"
                     /locus_tag="PKH54_04170"
     CDS             complement(5762..6217)
                     /gene="pgsC"
                     /locus_tag="PKH54_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOC99116.1"
                     /translation="MISEILPVSIGVGIVTGVLFSEAFGIATGGIVVPGYLALFVNQP
                     LVIALTVAAGFLTYVVVKALSMYLIIFGRRRTAVMILFGYVVGVLLNRFIGNQVGEFA
                     VIGYIIPGLIALWMDRQGTVQTLASLTIVTVIVRMVLIVTGFIDKGASL"
     gene            complement(6214..7449)
                     /gene="pgsB"
                     /locus_tag="PKH54_04175"
     CDS             complement(6214..7449)
                     /gene="pgsB"
                     /locus_tag="PKH54_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOC99117.1"
                     /translation="MINGFEAWGNPDMDGLLLLSIMAGGLVLIGMVENYFHRRALFSI
                     KTRIHVAGTRGKSSVTRILAAAMNHAGIRTAAKTTGTTARMITPEGREIPMFRPTGAN
                     LIEQKRVVAAAVGMGADAIVLECMALQPALHWVAEHMLTRATHAVITNARPDHLDVMG
                     PDDTDVARTLAAIIPPGRSVFTAERRNLDIIRDAAADRKATLFDVTPEEVAAVTPQDM
                     AGFSYKEHPENVALALKVLASLGIDRQVAIEGMWRAQPDPGALNDFILDYFGRRIIFV
                     NGFAANDPESSQTIWNAAVARHPGVDRVMAVFNLREDRPARTLQMVNDTDFWKDADKV
                     VVIGTGAYQFIRAAAKKGMDPSLFVDAADERVEGIFEKILENCSGSNLVIGMGNIGGF
                     GLPLVSYFRNRSEVRGLVQ"
BASE COUNT         1631 a         2469 c         2229 g         1504 t
ORIGIN      
        1 cgccgcaagg tggggcttcg aaaccgagat cccgcccgaa tccttcatgc acacatggcg
       61 ggggatcgcc acagacacca gcgtcggcgg cgactcggtc atctgcatgg gacaaggcaa
      121 cagcacatcc tggatcagcc gcgaaccgga cgggctcgtc gatatctttg ccggatcgct
      181 tggcggatcc cggggcgcgg aagacccgga aacggtcgcg atggacatga ttgcgcgcca
      241 gggcggcatc ggtgactggg tcaggccggg aaccagtgcg gtcatcccgc ttcggcgccc
      301 gtttgacggc atcgtcgcgc ccggattcat gctgcttggc gattcggcgt gcatggcgaa
      361 cccgatcaac ggttccggga tgacggttgc gatcgacgca gccatcatcg cggccgacac
      421 cgcccacgac gccatgatga agggtcgctt cgaagcatcg gatctgtggt catacccaag
      481 agatttcttc agggccaatg cggaactggc cttcctggac gccttgaagt ccttcgtgtt
      541 ttcggcgccg cgcgatccga tgctggactt cttccgacaa ggcggccttc gagggccggc
      601 cttctggaag atgcgccctg aattcggccc ggccggattc gccgccaagg ccggcgcgtt
      661 cgcgcggaca aaaggggcgg cggcccttct gcccccgatg gcgggaacac taacgcgcgc
      721 cgcgattgcc gagcgtcaca tggcgcttta cccgtcaagt ccatccggct tcaatcaatg
      781 gaaggcgata cgccgcagaa tcacaggctg aatgcgggca gaagcacctg gaaagtggtg
      841 ccgccggatc cggtttcctt cacgaacagg gcgccgccgt gcctggaaac gatctggctg
      901 gcggtcgaaa ggcccaggcc ggttcccgcg ccctcggcct tggtcgaaaa gaacggggca
      961 aatatccttt ccagattctc ttccgggatt ccacaaccgg tgtcgcggat ctcgatcgac
     1021 gcgatctggc cggattccag ccacggccac cttgccttga tccgcgtccg ctccggttcg
     1081 tcatcgatgc acgatgttct gaaagtcaac acgcctccgt cggccatcgc gtcccgcgca
     1141 tttaccgcaa ggttcacaag cacctgggtg aacgccgact tttccagccg aacgccgcac
     1201 tcacacccgc ttgactgcaa ttcgattcgc accgacgcac ccatcagcgg cttgaccatg
     1261 ttcatggtat cggccacgaa ctgatcaagg ggcatttcgt gcatcaccgg ctcggttcgc
     1321 ctggaaaaac tcaagatact ggcgaccaga tccttggttc tgtcggacgc gtccagcatc
     1381 gcgttcagat aggacgacgc ggtcggatcg gtcgcgacga acttccgcag gcactcgcca
     1441 tagccgttga ttatctggag catattgttg aaatcgtggg ccatggcacc ggccacccgc
     1501 cccagggaat caatcttctg caagtggtgc atctggaatt caagctcgcg aagcgcggtg
     1561 atgtcctgga cggttccatg cagctcctcg accacgccgt cttccatgat gaccggttcc
     1621 atgctcatca caaggtatct gatccgaccg ccgccaagct cgcgcctgat ttcgatctgc
     1681 cggaacctgc gcatcgtcac cgcgtcaagg atggccgact tgattacttc cagggtattc
     1741 gaccccagaa cctggccgat ccattcgggc gttacctcgt caacggcatc aaggccgacc
     1801 agatcgaatg cctcgggact ccagtaaatc tttctctgcc ggacattggt tgaccagtac
     1861 cccaggcggg aaacacgggc catgatgtca agctggacac ccttcatccg atcctggcga
     1921 atcgtttcga caatctcgct gacgttccgg acgaccgcca ttaccttgtc gccggcaaac
     1981 ggcaccatac gggcatggaa atagtgttcc acgccatcaa gatcaagccg gtaatggaat
     2041 tcccccttct gaccggtcag aaccacgttg ttcagggtac gcctggaaac atcggcgact
     2101 tccgcgggaa gaatgtcctc cagcctggcg cccagaaagt tcccgggcga agccagcagc
     2161 ttttcctcgt tcgcgatgtt gtatccgacg aaggtgcctt cacggtcaaa gatgaagacc
     2221 aggtcgggaa gggccgacac aagggcttca agacgttcct gcttttccct ggactccatt
     2281 tcggccatct tcctggccga aacatcgagc agggtacata ccacctcggt cacgttgcct
     2341 tcggcatcgc gggaaaacgg aagaaccacg ccctcgctgc atccgcctgc cgggaatccc
     2401 ctgtcctcgt aaagtccgcg gccgccggca aaagcgacct caatcgccgc ggcgacagtc
     2461 ggataaaggt cggttggagc ggcttcggaa agcttgcggc cacgccaggt ttcggaagaa
     2521 tagactgcgc ttccaatggc cccgccctcg atgaacgtga attcgtatcc gccggcgccg
     2581 tccctgacaa ggcggaagat caggccgttg atcgatcgaa gcatccgctc gcagtcaagg
     2641 gcgcgggtac gccagcaggc aatgtcatgc tctggcgagc cggccgaatc tgccgtggct
     2701 gccgaggtcg atgccctgtc atcccttccg atggacttga tggccctttt tgccctttca
     2761 gcctgtcgtg ttagcctttc gcccatgtct tcaaaagccc ccgggaacct ggccactcga
     2821 gtgtgacggc caatgccaga tccccgaaac agcagtccaa ttcaacttcc gccgggaaac
     2881 cgcgacctga cgcgtagtgt tcagacatag attattaccc ggaggccctt tcggcaacac
     2941 aaatcgtgaa cacgcttgaa cggactgaag aagatttgat tttcgccgcg taccagggaa
     3001 gtccggccac ggggactcaa gttggcctgc gtgaaaggat gcccttcaac agcgtaagca
     3061 aaggtccgga atcacgattc cggtattcca ggccgcccag gatcaccttc cgaaccttca
     3121 tgaacttcac ttcggccggc ggcgtgtcaa gcgggttccg atccagcacg accatgtcgg
     3181 cgaatttccc gggttccagc gaccccctgg aaacatcgtc aaaagtgctc ctgtagcctt
     3241 caatcgtgaa aagccgcagc gcctgccgca cggacagcga ctgttcggga acatagtggt
     3301 tgcacgccgc gtgcatcccg gcgatcgcgt cgggcagggt gcacggcgca tccgacccgc
     3361 cactgacgtg gattcccatt tcaaccatcg tcttgagcgg cgaaatcaac atcgcgcggt
     3421 cgccgagtat gtcggttatg tattcaagcg gctcaagctc ccactccagg aaggcgggct
     3481 gcaacggaag gtggatgccc agatcggcac acctgcgaag gccttccatg gtcggcaggc
     3541 atgcatggat gattccgtgc cggtgatccg gccttggcgt gtcgcgaagc gccgcttcaa
     3601 ttccatcgac ggcctgttca aacgcggcat ccccgattgc gtgaacctgg atctgcaaac
     3661 ctgccctgtt cgcccggacg cagaaatccc gcaccttcgc cggttcataa aaaagcacgc
     3721 cgcggttgtc ggtgttcctg taaggctgaa gaagcgccgc gtccatgctt ccgaaacacc
     3781 cgtccagcgc ggtcgcgaaa cacccgccga tccgtggcag acgacggcga agcacctttc
     3841 cgatgtccat cgtctggaag aaaatcctca tctggaggcc ggaggatgtg ccccttgcca
     3901 cccacctttc cagatcgacg tcgagatcct tcgggaaacc gacgccggac acgtcgtcaa
     3961 tcttgcagat ccccgaagac acaagcgagt cggccgccga gatcatgttc cggacgacct
     4021 gcatcggcgg aattcgaccg gtaacgaagt cggtgcaagc gaaatatgct tcctggttca
     4081 tttcgccggt gtcgggatga aaaccgcgca gcccccggat atgcgatgga agcatcgcaa
     4141 tcagcctgga attcaggata catgcgtggc cgtcgtactt gacgatgaag accggcctgt
     4201 cgccgcaggc ggcgtccagt tcagcccgtg aaaccagccg ccgctcggcg acgctgtgga
     4261 tcgacgcccc gaacccgacg atgatgtcct ggcggttcgt ggttgcgaat tccttcagcc
     4321 ttgcctggat ttccgggatg ctgcgggcgg atctgacatc cagcccggaa tgaaacaacg
     4381 cgaatgacgc aaaatgaata tggctgtcgg caaatgacgg aatcagcgcg tggccatccg
     4441 gcagcacttc ccgcgccgcc ccgacgaaac gttccggcag ttcgttgccc gtccagacga
     4501 tgcggccgcc atcctcgacc aggaagcggc acactgaatc cgaagcgtcg caggtggcga
     4561 ccgtaccttc ataaaccttc atggcatcct cccggggggg cggtcaatca gaccatgcgt
     4621 tcgggaaccg aatcattgac actgggctgg gcatggcttg acttgaaaag tcggtatccc
     4681 cagtccatcc ggacaaaggc gatcatcaga agcaccagca ccgcaagaag accgccggca
     4741 agccaggggt tgtagttctg ggtgacgaaa atcgggccgg tgccggcctt ctggggcgag
     4801 gtcggattca ccggaagccc ccatcgctcg gcaaacgcat tgaactggga gaagtgaaaa
     4861 accgggatgc ttttctgtgc gaaacgcatc atgacggaat cgtcggaaga ctcaatgctg
     4921 gtagtaactt cggtgttcag gccggtcgtg aagatcttct tgccgaccga gcgccccagg
     4981 gaaacggtac cgccgccaat gttgatatag gcgcggatgt tgtcttcacc gccggccacc
     5041 cgatcgtaga tctccatccg cttttcgaag ctgtccttgt aattcgaaac caccagggcc
     5101 ggccggccgc tgcgcacgat ggaatcgtca atcagcgtga cggcttccgc ctcgagacca
     5161 agtgcgttgt cgtcgattcc gccgcgggtc ataacctcgg acggatacgg caggatgttg
     5221 cgggcaacca gtatcctttc gatgtcgggc cacatgaagt ccggaaaatt ggcgccccac
     5281 tgcgacgaac cgacgctggt gatgatgatc ggcttcagtc ccagcacatg aacggccgca
     5341 agcacgttga tgttcatggc cggaaaagac cccgtcatgc cgattgccac gacgtcgccc
     5401 cggttcagcc cggcccgctt gaacaactgc accacgacgg cggcgaagtt cgggttggcc
     5461 gaagtccttt tcgacgtgat gtaacccgga ttggtcgtca cggaggtcac cgcgtcaccg
     5521 agcattccgg tgccggcagg atcgtactca aggttgaccg ggtgcttcag gcgcacgcgc
     5581 tcggccttga caacgtcgta cgcctgggct gccagccttg ccgcgtcaag cttcaacttg
     5641 taattcttct gcctttcctg gatggtcacg ttttcaacaa gcagcacgcc cgcgacggca
     5701 accagggcga ccatgaccag aacgaatgtc gataccttcc tgggacgcca gtacagcttt
     5761 ttcacagcga cgcccccttg tcgatgaagc cggtgacgat caggaccatc ctgacgatca
     5821 cggtgacaat cgtcagcgac gccagcgtct ggacagtccc ctgacgatcc atccacagcg
     5881 cgatcaatcc gggaatgatg tagccgatca cggcgaactc accgacctgg ttgccgatga
     5941 aacggttcag aagcaccccg accacatacc cgaaaaggat catgacggcg gttcgccgcc
     6001 gcccgaaaat gatcaggtac atcgaaagcg ccttcacgac gacatacgtc aggaacccgg
     6061 cggccacggt cagcgcgatg accagcggct ggttcacgaa cagggccagg tatcccggaa
     6121 caacgatgcc gcccgtggcg attccaaacg cctcggaaaa caggacgccg gtgacgatgc
     6181 cgacaccgat cgacactggc aggatttcgg aaatcattgc accaaccccc tgacttcgct
     6241 gcgattacgg aaatacgaaa ccagcggcag cccgaaaccg ccgatattgc ccatgccgat
     6301 caccaggttc gacccggaac agttttccag gatcttttcg aaaatgcctt caacccgctc
     6361 gtcagcggca tcgacgaaaa gcgacgggtc catgcccttc ttcgcggcgg cccgaatgaa
     6421 ctgataggcg cccgtaccaa ttacgactac cttgtccgcg tccttccaga aatcggtgtc
     6481 gttgaccatt tgaagggttc tggccggacg atcctcccga aggttgaaaa ccgccatcac
     6541 gcgatcgacg ccagggtgcc tggcgactgc cgcgttccag atggtctggc tggattcagg
     6601 gtcgtttgcc gcgaatccgt tgacgaaaat gatccttcgc ccgaagtaat ccaggatgaa
     6661 atcattcaag gcgccaggat ccggctgggc ccgccacatc ccttcaatgg caacctgacg
     6721 gtcaattccc agcgacgcaa gcaccttcag cgcaagcgcc acgttttcgg ggtgttcctt
     6781 gtacgaaaag ccggccatgt cctggggcgt cactgccgcg acctcttccg gggtcacgtc
     6841 gaacagagtc gccttccgat cggccgcggc gtcacgaatg atgtcaagat ttcgccgctc
     6901 cgccgtgaac acgcttctgc caggggggat tattgccgca agcgtccttg cgacatcggt
     6961 gtcgtccggg cccatcacgt ccaggtgatc cggcctggca ttcgtgatga ccgcgtgcgt
     7021 ggcgcgtgtg agcatgtgtt ccgccaccca gtgcagcgcc ggctgaaggg ccatgcattc
     7081 aagcacaatc gcgtcggcac ccatcccgac cgctgccgcc accacgcgct tctgctcaat
     7141 caggttggcg ccggttggac ggaacatcgg gatctcgcga ccttccggtg tgatcatgcg
     7201 ggcggtcgtt ccggtcgtct tcgcggcggt cctgataccc gcgtgattca tcgccgccgc
     7261 aagtatcctt gtgacgctgg acttacccct ggttccagcc acatgaattc gagtcttgat
     7321 ggaaaacagg gcacgtcggt gaaaatagtt ttcgaccatg ccgatcagca cgagtccacc
     7381 ggccatgatt gacagaagca gcaggccatc catgtcggga tttccccagg cttcgaaccc
     7441 gttaatcatt acgcctttcg ggccttggat gtcaatcgaa tcgcgccccc tggcggtcga
     7501 agcggcagcg ggtgccaaaa attgtcacca cccatcgaca caacacgtcc gaaggctcca
     7561 aaatcgacct gaacagcggc ggattggaga tccggcccgc cagtgaatcc ggacgcccgc
     7621 gccctggcac gaatcaaaat tccctttaat aacgaagtca tgcaacccga gagaagaaca
     7681 ctccggtcat cccccgccga ctggcaccat tattgcagtt gttaaccgca agcggggacg
     7741 tcggggggct tcagcgccat atgttcgatc ggaattggcc cgaatctgcc agattgttgg
     7801 cagttcgggc cttttctttc tggacaggta ccg
//
LOCUS       DAOPPA010000091         7826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10397_length_7826_cov_3.926908, whole genome shotgun sequence.
ACCESSION   DAOPPA010000091 DAOPPA010000000
VERSION     DAOPPA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10397_length_7826_cov_3.926908"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PKH54_04180"
     CDS             <1..1816
                     /locus_tag="PKH54_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99118.1"
                     /translation="IAEILEIGLGSQVQGVQTQLNNRLAIEFLKQKDGDFLAAVFSEM
                     ARDVPMTMAIHLMAFFRHEQGRLKDSFDPVAFFEQKTGLKAPRFEDYQAGGRRARQSY
                     FQALDELAVQVMQKSAGYRAVKNRLREAAEPPRKPRVYKAAIETSTRVAGELIDSADA
                     ATRKWLERSRPTSTERDRCIAAWDSAFMACREALKADPWAFHQPWFKQIWQRSEQAVR
                     VASVVRGFQADLDDVRPWLTHFDGSGIAATCPTGQLGHARQQQLVDAAIGAIWAYPDD
                     RARIASDPLARLLMWQEDGNWDLTIVSAMGVITDGAYGREMAPAFDRLLSTRGISLRR
                     VPTGLFRTLEFNARAIISEVRQVQGPWAWLGYSQGCANCLRAEHIMMTGTPELRALAD
                     RIACRQMIFSAANGSVHATAGARLFTRMLIEGEKSLKHYQAVFSREFVSMFLKTGRGV
                     LDSRVSTALLAGLWSMTFDRAIDLHRDGVFAPGVPTSTIRAAVGWNELPDGLRYVFGM
                     HNRLMPGEDCDSQVATLDEVGHSTRVINARTAVLESCDMGSAAIRMHHWGPVHEEAAQ
                     GMTRDDVALHKYDSPADLYIFPWIDTCIRFGFIKPSER"
     gene            2025..3146
                     /gene="serC"
                     /locus_tag="PKH54_04185"
     CDS             2025..3146
                     /gene="serC"
                     /locus_tag="PKH54_04185"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOC99119.1"
                     /translation="MAPCNDKVHRVINFSAGPAGLPLAALEKARDELLDFEGSGMSIM
                     EMSHRGKIYDRVHTEAINLTRELMGIPDDYVVLFLQGGANLQFGMLPMNVLGNGKKAD
                     YIISGHWAKSAYKEAVKVAGAENVRAIASTESEDFRRLPRPSEYRINDDAEYVHICTN
                     NTIYGTQYHYVPETGKVPLVADMSSDIMWSPVDVKKYAMFYAGAQKNLGPSGLVLVVM
                     HKDFLAKCREDLPNMLQYKVHADKNSLFNTPPTFAIYLLKNVLEYNKSIGGLAAIRAN
                     NLKKGELLYGFIDANADYYRPWVTVKEDRSVMNVDFHLPNETLDAQFVAEGQKAGMVG
                     LKGYRTMGGIRVSMYNAVTVANIETLVGFMDEFRKRNPG"
     gene            3215..5242
                     /locus_tag="PKH54_04190"
     CDS             3215..5242
                     /locus_tag="PKH54_04190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOC99120.1"
                     /translation="MSIRNTLFPVAALLLLASCEFVSGETAAVQSPIIGGTAVTDGSW
                     PAVGGIVLYPDSMCSGTLISPRVVVTAAHCVKGENPTTFVLGYDMENGTEYAVQEAIY
                     NPDYDAGGYNDPGSGDVAVLVLEERITGITPARLFTGDFTAFNDQSIVFVGYGKRDVY
                     QPLYGKKYQVTLTIKDIDDYGFWNYTNQSNVKNTCGGDSGGPGFVSYNGEYYIGGFVS
                     SGDEYCVQDGYNMRADSNAEWILSQVEKYDPGFLGEPDCGNDICEYGEDSGNCAEDCP
                     VVEPECGNDVCEDGETEANCADDCATGPDPIWTECGSQGECPAGQFCIQVSQNAYYCT
                     ISCDDLELGGGCGYQDMACVALQDGGGACVPADIECGDGLCEYGETADSCQSDCAIDC
                     GDLSYEGCCDGTQAVYCNNGQFRRVECGPDYTCGWVVDGGSSYYFCTDGAASADPSGT
                     FPMSCEGYIFDSCGNGVCDNDETESSCPGDCKVVDSCGNDKCDNGETVVTCPEDCKVV
                     DSCGNNKCDNGETVVTCPEDCTTVDEDCGNEVCDDWESNLSCPIDCPLLPDNCGNGTC
                     DNDENTSTCPQDCPVQDPECGDTICESPEGCRDCPQDCGECPVGEDVIQAQDTITADD
                     SGQTESDTVGVASGGCSSTGANGAGTGTAGLLLTVLLAMTSIIVRRSRRFA"
     gene            5262..6293
                     /gene="mnmA"
                     /locus_tag="PKH54_04195"
     CDS             5262..6293
                     /gene="mnmA"
                     /locus_tag="PKH54_04195"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOC99121.1"
                     /translation="MNTAIAMSGGVDSSVAASILQEQGHNLFGVTFITGHEADADLTV
                     SRAAAAATTIGIPHFVFDASDIFESQVIQPCADAYSRGLTPNPCAICNPILKFGALLD
                     FCASLGADRLATGHYARILTAPDGGPALARGLDSRKDQSYFLFRVPRDVLSSVVFPLG
                     GLTKAQVRQRAAELGLPAATSDESQDVCFGNSERPLHEVLREITDRRGPPGNVVSPDG
                     TVLGMHDGIDAFTIGQRRGLRIATGTRAWVTRIDPGGDVVLSNDPSDLECTSLLARDV
                     VIGAGLDGSGPLACKIRYAHRAVPCTAGILPDGRLRVDFIEKVKAVTPGQASVVYSGD
                     LVCCGGWIE"
     gene            6290..>7826
                     /locus_tag="PKH54_04200"
     CDS             6290..>7826
                     /locus_tag="PKH54_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99122.1"
                     /translation="MTIKLQYDGDVRLDRLEDRIRMVGTSPATDVALSLLDELNGAVV
                     AGLSFQGTLRGVKVFSTLAGYVTPAQISSSQTAAASVTERARLAERLLASASSTWGTH
                     IGLLSAEDRIKAWEAVDNLFASVIAAHLHDEVIYAGFRHVDKCEFPPTATATILARRL
                     LTLGTVDHRFVQLIEFLNRNARFPQEMALARACGSRLDFQLQSALMKRSGALLEALVQ
                     LKSVDESVTETFGQDFFVDVSGTESREVAELLTKRVLAVKAEWNIRDQFANNYVEYII
                     GRIDAGAGNPEHAIRVFQQTARGGFASYDSTVFLALLATSEGRQELARQSIQELVSRY
                     AVPLDFSEAFGSVMTAWRRAGGDPAVLQKSFPMEELVELCRASRARIDSEFSDGVEED
                     RKRATDSFWLGRTERLLAGVGLALEPTDFDASSSPGHDSRLVRLPEKILVEAGIDQMP
                     GLSPIARATFVEAARSGVAASELIGQLFEIADNLKGDFGAMAKLVPAWAYSQSIALGR
                     LEDA"
BASE COUNT         1526 a         2298 c         2405 g         1597 t
ORIGIN      
        1 catcgccgaa atcctggaaa tcggcctcgg ttcgcaggtt cagggcgtcc agacgcagtt
       61 gaacaaccgc ctggccatcg agttcctgaa gcagaaggat ggggacttcc ttgcggccgt
      121 gttcagcgag atggcccgcg atgttccgat gacgatggcc atccacctga tggcattctt
      181 ccggcacgag cagggacgat tgaaagactc cttcgacccc gtggccttct ttgagcagaa
      241 gaccgggctg aaggcgcctc gcttcgagga ctaccaggca ggcggccgcc gtgcccgcca
      301 gagctacttc caggcgctgg acgagcttgc agtccaggtg atgcagaagt cggccggcta
      361 tcgcgcggtg aagaaccgtc ttcgcgaggc ggccgaacct ccaaggaagc cgcgcgtcta
      421 taaagccgct atcgaaacat cgacccgggt cgctggcgag ctgattgaca gcgccgatgc
      481 ggcaacgcgc aagtggctgg aacgttcgcg tccgacctcc accgaacggg atcgctgcat
      541 cgcggcatgg gattccgcgt tcatggcctg ccgagaggct ttgaaggccg atccgtgggc
      601 ttttcaccag ccatggttca agcagatatg gcaacgcagc gaacaggccg ttcgcgtcgc
      661 gtcggttgtc cgcggattcc aggcggatct ggatgatgta agaccatggc tgacacattt
      721 tgacggcagc ggtattgcgg cgacgtgtcc gaccggacag cttggccacg ccaggcagca
      781 gcagcttgtc gatgcggcaa tcggtgcgat ttgggcgtat ccggatgacc gcgcccgcat
      841 cgcgtcggat ccccttgcaa ggcttctgat gtggcaggag gacggaaact gggatttgac
      901 catcgtgtcg gcgatgggcg ttattaccga cggcgcctac gggcgtgaga tggccccggc
      961 cttcgacagg cttctgagca cccgcgggat ttcattgagg cgcgttccga ccggcctgtt
     1021 caggacgctt gaattcaacg ccagggcaat tatttcggag gttcgccagg tgcaggggcc
     1081 gtgggcctgg cttggatact cccagggctg cgccaactgc cttcgtgccg aacatatcat
     1141 gatgacgggc acccccgaac ttcgcgcgct ggccgacagg atcgcctgcc ggcagatgat
     1201 attttcggcc gcgaacggtt ccgttcacgc aacggccggc gccaggttgt ttacccggat
     1261 gctgatagaa ggcgagaagt ccctgaaaca ttaccaggcc gtcttttcca gggaattcgt
     1321 ttcaatgttc ctgaagacgg gcaggggggt tctggacagc cgcgtttcca cggcgcttct
     1381 tgccggcctg tggtcgatga ccttcgacag ggcaatcgac cttcatcgtg acggtgtttt
     1441 cgcgcctggc gtgccgacat ccacgattcg agccgccgtc ggctggaacg agcttcccga
     1501 cgggcttcgg tacgttttcg ggatgcacaa tcggttgatg ccgggtgagg attgcgacag
     1561 ccaggtcgcg acccttgacg aagtcggcca ttcaactcgt gtcatcaatg cgcgcacggc
     1621 cgtccttgaa tcctgcgaca tgggaagcgc cgccatccgg atgcatcact gggggcccgt
     1681 ccacgaagag gctgcccagg gcatgacccg ggacgacgtc gccctgcaca agtacgacag
     1741 ccctgccgac ctttacatct tcccgtggat cgacacgtgc atccggttcg gattcatcaa
     1801 gccttcagaa cgctgaccgg aatcgggcgt ccgcctttgg tgaagcatcg gcgacatccc
     1861 cccccaccat aacaatttcg ggttcttgcc gaaccgatcg ctgcgaaagc cacttggcag
     1921 ttactaacag gctgggctag tatcaacgcg gaccgacggg tagtcccgtt ccccgcggtt
     1981 catggctgta cgccgacggg gctgaaaagt ctttggaggt tcttatggct ccctgtaacg
     2041 acaaggttca ccgcgtaatc aacttctctg ccgggcccgc tggtcttccc cttgcagcgc
     2101 ttgaaaaggc gcgtgacgaa ctgctggact tcgaaggcag cgggatgtcc atcatggaaa
     2161 tgtcccacag gggcaagatt tacgacaggg tgcacaccga agcgattaac cttacgcggg
     2221 aattgatggg aattcccgat gactacgtcg ttcttttcct gcagggcggc gcgaacctgc
     2281 agttcggcat gcttccgatg aacgtccttg gcaatgggaa gaaggctgat tacatcattt
     2341 ccggtcactg ggcgaaatcg gcgtacaagg aagccgtcaa ggtcgccggc gcggaaaacg
     2401 tcagggccat cgcatcgacg gaatccgagg attttcgcag gcttcccagg ccgtctgaat
     2461 accggattaa cgacgacgcc gagtacgttc acatctgcac caacaacaca atttacggca
     2521 cccagtacca ttacgttcca gaaaccggca aggttccact tgtcgcggat atgtcatcgg
     2581 acatcatgtg gtcgccggtc gatgtgaaga agtacgcgat gttctatgcc ggcgcccaga
     2641 agaacctggg tccgtccggg ctggttctgg tcgtgatgca caaggacttc ttggcgaagt
     2701 gccgcgaaga ccttccgaac atgctccagt acaaggttca cgcggacaag aacagcctgt
     2761 tcaacacccc gccgaccttc gcgatttacc tgctgaagaa cgttctcgaa tacaacaagt
     2821 cgattggtgg tcttgccgcc attcgcgcga acaacctgaa gaagggcgaa ctgctttacg
     2881 gcttcatcga cgcgaacgcc gactactatc gtccgtgggt cacggtcaag gaagaccgtt
     2941 cggtcatgaa cgtcgatttc cacctgccga atgaaacgct cgacgcccag ttcgttgccg
     3001 aaggccagaa ggcgggcatg gtcggcctga agggataccg cacgatgggc ggcatccgcg
     3061 tgtcgatgta caacgcggtc acggttgcga atatcgagac cctggtcggc ttcatggacg
     3121 aattccgcaa gcgcaaccca ggttgaaaca ccagccctat ctgcctataa tccgaccggc
     3181 tgtatctttc gaacttcctt caggttgtga aaaaatgtcc atccgcaata cgctctttcc
     3241 cgtggctgcg ctgttgcttc ttgcttcctg tgaattcgtg tccggcgaga cagccgccgt
     3301 ccagtccccg attatcggcg ggactgcggt gaccgatggt tcgtggcccg ccgtcggggg
     3361 gatcgttctt tatcccgatt cgatgtgttc cgggacgctg atttcaccgc gcgtcgtcgt
     3421 gaccgcggcc cactgcgtca agggcgaaaa tccaaccacg ttcgtgctcg gttacgatat
     3481 ggaaaacggc acggagtacg ccgtccagga agccatctac aacccggact acgatgccgg
     3541 cgggtacaac gaccccggat ccggcgacgt tgcagtcctt gtccttgaag agcgcatcac
     3601 cggcatcacg ccggcgcggc tttttaccgg cgatttcacg gcattcaatg atcagtcgat
     3661 cgttttcgtc ggatacggca agcgtgatgt ctatcagccg ctatatggca agaagtacca
     3721 ggtaacgctg acgatcaagg acatcgatga ttacggtttc tggaattaca cgaaccagtc
     3781 aaatgtgaag aatacgtgcg gcggggattc gggtggcccg ggatttgtca gttataacgg
     3841 agagtattac attggtgggt tcgtatcttc cggcgatgaa tactgcgtcc aggacggata
     3901 caacatgcgt gccgacagca acgccgagtg gattctttcg caggtcgaga aatacgatcc
     3961 cggtttcctt ggcgaaccgg attgcggcaa cgatatctgt gagtacggcg aggactccgg
     4021 caactgcgcc gaagactgcc cggtggtcga gcctgaatgc ggcaacgatg tctgcgagga
     4081 tggcgagacc gaagcgaatt gcgccgatga ctgcgcaacc gggccggatc cgatctggac
     4141 cgagtgcgga agccagggcg aatgtcccgc cggccagttc tgcatccagg tcagccagaa
     4201 cgcctattac tgcaccatca gttgcgatga cctggaactg ggcgggggat gcggctatca
     4261 ggatatggca tgcgtcgcgc tgcaggatgg cggcggcgct tgcgtcccgg ccgatatcga
     4321 atgcggtgac gggctttgcg agtacggcga aaccgccgac tcctgccagt ccgattgcgc
     4381 catcgattgc ggcgacctga gctacgaggg ctgctgcgac ggcacgcagg ctgtttactg
     4441 caacaatggc cagttccggc gggtcgagtg cggtccagac tacacctgcg ggtgggtcgt
     4501 tgatggcggt tcgtcgtatt acttctgcac cgacggcgcg gcctcggccg atccgtccgg
     4561 gacattcccg atgtcctgcg aaggctacat ttttgattcg tgcggcaacg gcgtttgcga
     4621 caacgatgaa accgaatcaa gctgccctgg cgactgcaag gtcgtcgatt cctgcggcaa
     4681 cgacaagtgc gacaacggcg agaccgtagt gacgtgcccg gaggactgca aggtcgtcga
     4741 ttcctgcggc aacaacaagt gcgacaacgg cgagaccgta gtgacgtgcc cggaggactg
     4801 cacgaccgtt gacgaggatt gcggcaacga agtgtgcgac gactgggaat cgaatctgtc
     4861 gtgcccgatc gattgtccgt tgttaccgga caactgcggt aacggcacat gcgacaacga
     4921 cgagaacacg tccacttgcc cgcaggactg cccggttcag gatcccgaat gtggcgacac
     4981 catctgcgag agccccgagg gctgccgcga ttgtccgcag gattgcggcg agtgcccggt
     5041 tggcgaagac gttattcagg cccaggacac gatcactgcg gacgattccg gccagaccga
     5101 aagcgacacg gttggcgttg catccggcgg gtgcagttcg actggagcca atggggccgg
     5161 tacgggtact gcaggtttgc ttcttaccgt gcttctcgcc atgacgtcca tcattgttcg
     5221 gcgttcgagg cggttcgcat gaccgcccgg gacattgatt catgaacaca gccatcgcga
     5281 tgtcaggcgg cgtcgattcg tcggtggccg catcgatact gcaggaacaa ggccataacc
     5341 tgtttggagt cactttcatc acgggtcacg aagcggacgc cgacctgacc gtttccaggg
     5401 ccgccgccgc cgcaacgacc atcggcattc cccacttcgt gttcgacgca tcggacatct
     5461 tcgaaagcca ggtcatccag ccatgcgcgg acgcctattc acggggactg acgccgaacc
     5521 cctgcgccat ctgcaacccg atcctgaagt tcggtgcgtt gcttgatttt tgcgccagcc
     5581 tcggggcgga ccgactggca acaggacatt atgcccgcat cctgaccgcc cccgatggcg
     5641 gcccagcgct tgcccgcggc ctggattccc gaaaggatca aagctatttc cttttcaggg
     5701 tgccaaggga cgtcctttcc agcgtcgtgt ttccccttgg cggcttgaca aaggcgcagg
     5761 tcaggcagcg ggccgccgaa ctcggcctgc ccgcggcgac gtccgatgaa agccaggacg
     5821 tctgcttcgg gaattccgaa cggccgcttc acgaagtgct gcgggaaatc accgacaggc
     5881 gggggccacc tggaaacgtt gtgtccccgg atggtaccgt gctcgggatg cacgacggca
     5941 tagacgcctt cacgatcggc cagcggcgcg gtcttcgcat cgcgaccgga acgcgggcat
     6001 gggttacccg cattgacccg ggcggcgacg tcgtactttc aaatgacccg tcggatcttg
     6061 aatgcacgtc gttgctggct cgcgatgtcg tcatcggtgc cggcctggac gggtctgggc
     6121 cgcttgcctg caagatccgc tatgcccacc gcgccgtccc ctgtacggcc ggcatccttc
     6181 cagacggcag attgcgcgtt gatttcattg aaaaagtaaa ggcagtcaca ccgggacagg
     6241 cgtctgtagt atattcaggc gatcttgttt gttgtggcgg ctggatcgaa tgaccatcaa
     6301 gcttcagtat gacggcgacg tacggctgga tcggctggaa gaccgcatcc gcatggtggg
     6361 gacaagtccg gccaccgacg ttgccctttc cctgcttgat gaattgaacg gggccgttgt
     6421 cgcggggctg agtttccagg ggacccttcg cggcgtcaaa gtcttttcaa ctcttgcagg
     6481 ttacgtcacc ccggcccaga tttcatcgtc ccagactgcc gccgcgtccg tgaccgagcg
     6541 cgcgcgcctg gcggaacggt tgcttgcatc cgcaagttcg acgtggggaa cacatatcgg
     6601 gctgctgtcg gccgaagacc ggatcaaggc gtgggaagcg gtcgataacc tgtttgcatc
     6661 ggtaatcgcc gcacatcttc acgacgaggt catttacgcc gggttcaggc acgtcgataa
     6721 gtgtgaattc ccgccgaccg cgaccgcgac catccttgcc aggcggctgc ttacgctggg
     6781 aacggtcgat cacaggttcg tgcagcttat tgaattcctg aaccggaacg cgcgctttcc
     6841 ccaggagatg gccctggctc gcgcctgcgg ttcccgtctt gatttccagt tgcagtcggc
     6901 gctgatgaag cgctccggcg ctttgctgga agcccttgtc cagttgaaat cggtcgatga
     6961 atcggttacc gagacattcg gccaggactt tttcgttgat gtttccggga ccgaaagtcg
     7021 ggaagttgcc gaacttctta cgaaacgtgt tcttgccgtg aaggcggaat ggaacatccg
     7081 cgatcagttc gccaacaatt acgtcgagta catcattgga cgcatcgacg ccggcgccgg
     7141 caatccggag cacgcaatca gggtattcca gcagactgcc cggggcggct tcgcgtctta
     7201 cgattcgacc gtgtttcttg cattgctcgc aaccagcgag gggcgtcagg agcttgcccg
     7261 ccaatctatt caggaacttg tttccaggta tgccgtcccg cttgattttt ccgaggcctt
     7321 cgggagcgtc atgaccgcct ggcgaagggc ggggggcgat ccggcggtgc ttcagaagtc
     7381 cttcccgatg gaggaactgg tcgaattgtg cagggcctcg agggcacgca tcgattcgga
     7441 gttttccgat ggtgtcgagg aggaccgaaa aagggccacc gactctttct ggctgggccg
     7501 cacggaaagg ctgctggccg gcgtcggcct cgcgcttgaa ccgaccgact tcgacgcttc
     7561 gtcatccccc ggacatgact cgcgtcttgt gcggttgccc gaaaagattc tggtcgaggc
     7621 cgggatcgac cagatgcccg gcctttcccc gatcgcgcgc gcgacgttcg tcgaggccgc
     7681 gcggtcaggc gtggccgcgt ccgaactgat tggccagctt tttgagattg ccgacaacct
     7741 gaagggcgat ttcggtgcca tggcaaagct ggttccggcg tgggcatatt cacagtcgat
     7801 tgccctgggg cgacttgaag acgcca
//
LOCUS       DAOPPA010000092         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10478_length_7770_cov_3.879585, whole genome shotgun sequence.
ACCESSION   DAOPPA010000092 DAOPPA010000000
VERSION     DAOPPA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_7770_cov_3.879585"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKH54_04205"
     CDS             <1..535
                     /locus_tag="PKH54_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC99123.1"
                     /translation="SPPEGLRGESGEMWVQDCAAATQNILLAATAQGLGAVWIGIHPV
                     TDRVAAVRNILEIPQDKTPFSIVSIGWPEGTPTPKDKFKPEKVFNGKWGTAFEFSKPA
                     ATPPDAIDAPAVQAPEPAPAADETGTVPVPPPDAAPAAEPAAVKPPAPVIPGLPADTM
                     PRAAAEAAPEQPAPTQN"
     gene            complement(642..4388)
                     /locus_tag="PKH54_04210"
     CDS             complement(642..4388)
                     /locus_tag="PKH54_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOC99124.1"
                     /translation="MNRFAPLIIVISVLLAPQVMASPVNFKPIDAVQPDGSPVELFVT
                     GDEYYRYVHDAAGYPVGQDEDGWYLYIENSDGRGLSELPVGLGDPSISGIRAVPGPPD
                     RPAEIREIVQGHLAVKAAIRESASVGVKRLGGPFRGTINEVVIFVRFAGEAEFEDLSL
                     TFDDMFNASDPEDSSMSRYFSEVSYGRLDVRGHLYPGSSGEAVASYQDSKPRRYFQIR
                     SARNPDGYTNQGEGYNRLSAMLNRAIEAIKPQIPSNLDLDLDNDGLAENVVFILSGDA
                     DEWADVLWPHSSILMYENSINGARLFQYNLQFRDWLLERAVGVLAHEFFHAIGAPDLY
                     HYSHDGIDPAGPWDLMEHTAEPPQHMMAWMKYKYGFFIDEVPVVTEPGRYTIKSLQNA
                     TDNVVRIPVPGQKFQYVALEYRVKDGVFEGGLPDSGLLAYRIDDANNGNAQGPPDEVY
                     IFRPGGSPAVNGEVGEAAISADELPASITPESNPYLFQQNGDPIGIRIYDIEFDGDSV
                     SFSVCPDIPDCLGIECGDDGCGGSCGDCDDGNDCTLDQCVEGRCEFQFAPPASPCGLG
                     ELCINGGVCDGSGVCVADVPAECDDGNDCTMDLCADGGKFVDLGVDRFEDVMPTDALS
                     AGIDGDQVVAGNISIGFDFPFMGDSFGWVKVFDNGLIQLSRDGVVDEDDELVAAESAL
                     NQDIPSELRPDNFIALFWDDLECRLADKCVVAYKVLGQAPDRRLIVQYNKVRRVEHPG
                     WLMRFQAALFESGRIELRYGTMGHEQGASATVGIEGVDGTGAVKFGFNQRVLRSGMTL
                     AYTPDPWQCVNRPKSGNCLMDGECVEAGTVDPDNPCMECRPLVNFGGWSADDANVCSD
                     DNLCTAVDRCVAGTCTGTEPVVCKPTSQCYTAGKCEPQTGLCPETMKEDGTLCNFDSN
                     GCTVGDACVGGECIVGTPADCSSRDSECAVGKCTSRSSNSFRCDQDKAAKAGQLCGSG
                     PSDCSGQDVCDASGVCQPNHFDSGIECRQAAGPCDRAEFCDGAGACPEDGFLPDGEAC
                     EGEGNECFAGQCVPYQRQAQCETAFSLRWTDEDGGQTTDGAGSLERGNFGHPHGDCMS
                     GGVRALWFEFPTQEGYSYTIDVSSPDGADVAVSLMQDCDSDACDGFVNAFEGAGTETI
                     ALGAPGFRPSVLLGVFRMDETSPAGVLVQVAREPVVVVDDVVEGDAEDAVGQADAVGD
                     DVVTTPSGGCSTGAGRNHTVPFGILFMMVFAAFVIARSRRISRR"
     gene            4551..>7770
                     /locus_tag="PKH54_04215"
     CDS             4551..>7770
                     /locus_tag="PKH54_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC99125.1"
                     /translation="MESRKLTDGGNQAAVDRLLRESPILTSMLNHLSDVIFYKDAKCR
                     YVLVNRAFADLVAREINDIPGGDDFDFFDKSVASAFRKEDLQVLESGQPLFIDSPAYA
                     PGGLRRIFETVKLPFKDADGTIQGVIGVARDITARKAYQAEVEDHAREMGCLVSLLQY
                     FATFDVGLSSMMKHAVQVIPTAWAVPDDIKVRIMLDGAVEETPGTLEDMQELTADIQF
                     DRHYYGYVSLGYYGLDRATHKDFRVGRGPRLLDVLANQLAQVAYRVGVHRALIESEYR
                     HRILTEHSPVGLLRTDDQGQIIDCNQAAATIFETTADRLRGLNCFDDVTESGLKQVIP
                     IALAGNRAEFEGPFRSAYGHTRYLRIVSMPLKSPSGSPETISTLEDVSERKRNEEALA
                     ASQASLAEAQRIAHVGSWEIDFTTGRLRASAECKRIFQVDQSVGEVESHQFLKFFKGD
                     FRDQIEARLKRAANPDTETRLYSHVALELPDGSEKWVNIHSEVEKDSLGIPKRIFGIV
                     HDVTQAHRTEAELRDARDQAEAASRAKSAFMANVSHEIRTPMNAIIGMTSLVLDSRLS
                     DRQRDYLNKINGASRALLGIIDDILDFSRIEADRMVLQNVDFSVENVLTEVIDINEQR
                     IEKKGIELHTDFDCDIPQLVRGDPLRLHQVLNNLVGNAAKFTMHGDIIVKVSQQARST
                     DNGTDGRIFLQFDIIDTGIGMTREQIADLFMPFSQLDGSTTREFGGTGLGLALCRKLV
                     ALMKGTISVESEPGEGSTFSVRIPFDQASSGIQEGSGPAPSPALAGRRALIIDDNPTF
                     RKIITRRLEHCSMATATAVSGEDAIETVKKASAEGKPFDIILVDLEMPGIDGIATARE
                     LMIRTGTRTPMVLMTTAYMFNDQIQAQAARVFDSVLFKPLLPTSLGAALGKVLLKSAS
                     EIEPDRKRSYNFEGRHILLVEDNRINQQVALELIRKTGARVSLAENGREALAMAGREQ
                     FDLILMDIQMPEMDGLDATMQIRRLNRDWIAQVPIIAMTAHGMSQHVENSIRAGMNAH
                     LTKPVHPDQLFETLARWLKGASSVVTTAAPRQSSIDLQPPRFQ"
BASE COUNT         1668 a         2476 c         2209 g         1417 t
ORIGIN      
        1 ttcacctcct gaaggcctgc gcggcgaatc cggtgagatg tgggttcagg actgcgccgc
       61 ggcaacccag aacatcctgc ttgccgcgac cgcccagggc ctgggtgcgg tctggatcgg
      121 catccacccg gtgaccgatc gcgtggccgc cgtcaggaac atccttgaaa taccgcagga
      181 caagaccccg ttcagcatcg tttcgattgg ctggcccgag ggcaccccga caccaaaaga
      241 caagttcaag ccggaaaagg tgttcaacgg caagtgggga acggccttcg agttctcgaa
      301 gccggcggcg acgccgcctg acgcgattga tgccccggct gttcaggctc cggaaccggc
      361 cccggccgcg gatgaaaccg gcacggttcc agttccgccc ccggatgcgg cacccgctgc
      421 cgaaccggcc gccgtcaagc ccccggcccc ggtgattccc ggtcttcccg ccgacacgat
      481 gccacgcgcg gcggcagaag cggcaccgga gcagcccgca ccgacgcaga actgacaggc
      541 acagcaaggc ggcaggtaca gcccgcatcc acccggaact gacaggaatc acgaagacgc
      601 gggaaaagtc ggcgcgatga acagaccggg aatcggttcc gtcaccgtct ggaaatgcgg
      661 cgcgaccgtg cgattacgaa ggcggcaaaa accatcatga acaggattcc aaaggggact
      721 gtgtgattcc ggccggcacc agtgctgcaa ccgcctgacg gggtcgtgac gacatcgtcc
      781 ccgaccgcat cggcctggcc gactgcatct tccgcatcac cctcgacaac gtcatccacc
      841 acgacgaccg gttcccgggc gacctggacc agcacgcccg ccgggcttgt ttcatccatc
      901 cggaagaccc ccagcagaac ggacggcctg aatccgggcg cgcccagcgc tatcgtttcg
      961 gttcccgcgc cctcgaaggc attcacgaat ccgtcgcatg catccgaatc gcaatcctgc
     1021 ataagggaaa ccgcaacgtc ggcgccgtcc ggcgaactga catcgatcgt gtacgagtac
     1081 ccttcctgcg tcgggaattc gaaccacagc gccctgacac cgccggacat gcagtcgcca
     1141 tgcgggtgac caaagttgcc ccgctccagg cttcccgcgc catcggtggt ctgtcccccg
     1201 tcctcatccg tccagcgcag agagaacgcg gtttcgcact gggcttgccg ctggtaaggc
     1261 acacactggc cggcgaagca ctcgttgcct tcgccctcgc acgcttcgcc atccggcagg
     1321 aatccatctt ccggacaggc gccggcgccg tcgcagaact cggcccgatc acacggaccg
     1381 gccgcctgcc gacactcgat cccggaatcg aaatggttcg gctgacagac gcccgacgca
     1441 tcgcacacgt cctgcccgct gcaatccgat ggaccgctgc cgcaaagttg tccggccttc
     1501 gccgccttgt cctggtcaca ccggaaggaa ttgctgctgc ggctggtgca ctttccgacc
     1561 gcgcattcgg aatcccttga cgaacagtcg gcaggcgtcc cgacgatgca ctcgccaccg
     1621 acgcaggcat ccccgacggt acagccgttg ctgtcaaaat tgcacagggt tccgtcttcc
     1681 ttcatcgtct cgggacacaa tccggtctgg ggttcacact tcccggccgt gtaacactga
     1741 ctggtcggct tgcaaaccac cggctcagtt ccagtgcatg tcccggcgac gcatcgatca
     1801 acggcggtgc acaggttgtc gtcactgcac acgttggcat catccgcgga ccaccccccg
     1861 aagttcacca gcggcctgca ttccatgcat ggattgtccg gatcaaccgt tcctgcttcg
     1921 acgcactcgc cgtccatcag gcaattgcca ctcttcggcc tgttgacgca ctgccaggga
     1981 tcgggggtat aggccagcgt cattcccgat cgcagcaccc gctggttgaa cccgaacttc
     2041 acggcgccag tcccgtcgac gccctcgata cccactgtgg ccgaggcacc ctgttcgtgc
     2101 cccatcgtcc cgtaacgcaa ctcgatgcgt ccgctctcga aaagggccgc ctggaaccgc
     2161 atcaaccaac cgggatgctc gaccctgcga accttgttgt actggacaat cagccgtctg
     2221 tcgggtgcct ggcccagaac cttgtacgcg accacgcact tgtcggccag cctgcattcc
     2281 aggtcgtccc agaaaagcgc gatgaaattg tccggcctca gttccgaagg aatatcctga
     2341 ttcaacgccg attccgcggc caccagttcg tcatcttcgt caaccactcc gtcccttgac
     2401 aactggatca atccgttatc aaagaccttc acccatccga aagaatcacc catgaacgga
     2461 aaatcgaagc cgatcgagat gttgcccgcg accacctgat ccccgtcaat gccggcggac
     2521 aacgcatctg tcggcatcac gtcctcgaag cgatccacac ccagatccac gaacttgccg
     2581 ccgtcggcgc acaaatccat cgtgcagtcg tttccgtcat cacactctgc cggcacgtcg
     2641 gccacgcaga cgccggaacc gtcgcagacg ccgccattga tgcagagttc gccaagaccg
     2701 cacggcgatg cgggcggagc gaactgaaat tcgcagcggc cctccacgca ctgatcaagc
     2761 gtgcagtcat tcccgtcatc gcagtcgcca cacgacccgc cgcaaccgtc gtcgccgcat
     2821 tcgattccaa ggcagtccgg tatatctgga cacacgctga aggaaaccga gtcgccgtcg
     2881 aattcgatgt cgtaaatgcg aatcccgatc ggatccccgt tctgctggaa aagataagga
     2941 ttactttccg gggtgatgga agccggaagt tcgtcggcgg aaatcgcggc ctcgccaacc
     3001 tcaccgttaa cggccggcga tccgcccggc ctgaagatat agacctcatc cggcggcccc
     3061 tgggcgttcc cattgttcgc atcatcgatt cgatatgcaa gaagaccgga atccggcagg
     3121 ccgccctcga acaccccgtc cttcaccctg tattccagcg cgacgtactg gaacttctgc
     3181 ccgggcactg gaatcctgac cacattgtcg gtggcgttct gcagggactt gatcgtgtat
     3241 cgtccaggct cggtaacaac tggcacttcg tcgatgaaga agccgtactt gtacttcatc
     3301 caggccatca tgtgctgggg cggctcggcg gtgtgttcca tcagatccca cggacctgcc
     3361 gggtcgatcc cgtcatggga atagtgataa agatccgggg cgccgatagc gtggaagaat
     3421 tcatgggcaa gcacgccgac agccctttca agcaaccagt cccggaactg caggttgtac
     3481 tggaacagcc tggccccgtt gatgctgttt tcgtacatca ggattgaact gtgcggccac
     3541 aggacgtcgg cccattcgtc ggcgtctccg gaaagaatga agaccacgtt ttcggccaga
     3601 ccgtcgttgt ccagatccag atccagattc gagggaatct gtggcttaat cgcctcgatg
     3661 gcgcgattca gcatcgctga cagccggttg tagccttccc cctggttcgt gtagccgtcc
     3721 ggattgcggg cgctgcggat ctgaaaatat ctccgtggct tgctatcctg gtaggaagcg
     3781 accgcctcgc cggatgaacc gggatagagg tgtcccctga catccaggcg tccgtacgag
     3841 acctcggaaa aatagcggct catcgacgag tcttccgggt ctgaagcatt gaacatgtca
     3901 tcgaacgtca gtgaaagatc ctcgaactcc gcttcgcccg cgaagcggac aaaaatcacg
     3961 acctcgttga tggtaccgcg gaaaggtccg ccaagccttt tgacgccgac ggaagcggat
     4021 tcgcggatcg cggccttgac ggccagatgg ccttgcacga tttcacggat ctcggccggc
     4081 cgatccggcg ggccgggcac ggcgcggatg ccggagatgg atggatcgcc aagtcccacc
     4141 ggcagttccg acaatccgcg cccatcagag ttctcgatgt aaaggtacca gccatcctca
     4201 tcctggccga ccggataccc cgccgcgtca tgaacatacc gataatactc atccccggtc
     4261 acgaacagtt cgaccggact tccgtcgggc tggacggcgt cgattggctt gaaattgaca
     4321 ggcgatgcca tgacctgggg cgccagcaac acggaaatca cgattatcaa gggagcaaaa
     4381 cgattcatct ggttggaagc cctccgcggc gacgtcatat tcgacatcaa acggactttg
     4441 tgatcaatgg cttttccgtc gtcctgttca ggcatacaat gccggttgca ggcaatcgct
     4501 tccgcgctcc agttttccgg acggcgggcc cgccagagtc gagggagcag atggaatcac
     4561 gcaagttgac ggatggcggc aaccaagccg ccgttgacag gctgcttcgg gaaagtccga
     4621 tcctgacatc catgctcaac cacctttccg acgtaatctt ctacaaggac gccaaatgcc
     4681 gttacgtcct tgtcaaccgt gcgtttgccg atcttgtcgc ccgcgagatc aacgacattc
     4741 cgggcgggga tgacttcgac tttttcgata aatccgtcgc gtcagcgttc cggaaggagg
     4801 atcttcaggt tcttgaaagc ggccagcccc ttttcatcga cagcccggcg tacgcgccag
     4861 gtggcctgcg ccggatattc gagaccgtga agcttccatt caaggacgcc gacgggacaa
     4921 tccagggcgt catcggcgtt gcccgcgaca tcacggcaag aaaggcttac caggccgagg
     4981 tggaagacca cgccagggaa atgggctgct tggtgtcgct gcttcagtac tttgccacct
     5041 tcgacgtcgg gctgtcttcg atgatgaagc acgccgtcca ggtcatcccg accgcgtggg
     5101 ccgtgcccga tgacatcaag gttcgaatca tgctggacgg cgcggtcgag gagacgcccg
     5161 gcaccctcga ggacatgcag gaactgacgg cggacatcca gttcgacagg cactattacg
     5221 gatacgtgtc cctgggatac tatggactgg acagggccac tcacaaggac ttccgggtcg
     5281 gcagggggcc aaggctgctt gacgtcctgg cgaaccagct cgcccaggtg gcataccgcg
     5341 tcggggttca ccgggccttg attgaaagcg aatatcgcca ccgcatcctt acggagcatt
     5401 cgcccgtcgg actccttcga accgacgacc agggccagat catcgactgc aaccaggctg
     5461 ccgccaccat attcgaaacc acggcggatc gccttcgcgg cctgaactgt tttgacgacg
     5521 tgaccgaatc cggcttgaag caggtcatcc cgattgcact tgccggcaac agggccgagt
     5581 tcgaggggcc tttccgatcc gcttacggac acacaaggta cctgcgaata gtgtccatgc
     5641 cgctgaagtc cccgtcggga agcccggaaa cgatttcaac ccttgaagac gtgtccgagc
     5701 ggaaaaggaa cgaggaagcg cttgccgcca gtcaggcatc tcttgctgaa gcccagagga
     5761 tcgcgcatgt gggaagctgg gaaatcgact tcaccacggg aaggcttcgg gcaagcgccg
     5821 aatgcaaacg catcttccag gtcgatcagt cggtcggtga agttgaatca catcagtttc
     5881 tgaaattctt caagggtgac ttcagggatc agattgaggc ccgcctgaaa agggccgcga
     5941 accccgacac cgaaacccgg ctttattcac atgtcgccct tgaactgcct gacggttccg
     6001 agaagtgggt aaacattcac tcggaggtgg aaaaggacag cctcggcatc ccgaagcgga
     6061 ttttcggaat cgttcacgac gtgacccagg cacatcggac ggaagccgaa ctgcgcgatg
     6121 ccagggatca ggccgaggcc gcgagcaggg ccaaaagcgc cttcatggcc aacgtcagcc
     6181 acgaaatccg taccccgatg aatgcaatca tcggaatgac cagcctggtg ctggacagcc
     6241 gtctcagcga tcgccagcgc gactacctga acaagatcaa cggcgcctcc agggcacttc
     6301 tgggaatcat cgacgatatc ctggatttct cccggatcga ggctgaccga atggttctgc
     6361 agaacgtcga tttctctgtg gaaaacgtcc ttaccgaagt catcgacatc aacgaacagc
     6421 gcatcgaaaa gaagggcatc gaactgcaca ccgatttcga ttgcgacatt ccccagcttg
     6481 tccggggcga cccgcttcgc ctgcaccagg ttctgaacaa tctcgtgggg aacgcggcca
     6541 agttcacgat gcacggcgac atcatcgtga aggtgtccca gcaggcacgg tcaaccgata
     6601 acggaacgga cggccgcatc ttccttcagt tcgacataat cgacaccggc atcggaatga
     6661 ccagggaaca gatagccgac cttttcatgc cgttttcaca gcttgacggt tcgacgacgc
     6721 gcgagttcgg tggaaccggc cttggactgg cgctctgcag gaagctggtg gcccttatga
     6781 agggaaccat ctcggttgaa agcgagccgg gcgaaggcag cacgttctcg gtcaggatcc
     6841 cgttcgacca ggcatccagt ggtattcagg aaggttccgg cccggcgcct tctccggccc
     6901 ttgccggcag gcgggctctg ataatcgatg acaacccgac cttccggaaa ataatcacaa
     6961 gacgccttga acactgctcg atggcaacgg caacagccgt gtcgggcgag gacgcaatcg
     7021 agaccgtcaa gaaggccagc gccgaaggca aacctttcga catcatcctg gtcgatctgg
     7081 aaatgcccgg aatcgatgga atcgcgacgg cgcgagaact gatgatcagg accggaacca
     7141 ggacgccgat ggttctgatg accaccgcgt acatgttcaa cgaccagatc caggcccagg
     7201 cggccagggt gttcgacagc gtccttttca aaccccttct tccaacgtcc ctcggggccg
     7261 cgcttggcaa ggttctcctg aaatcggcca gtgaaattga acccgaccgg aaaaggtcat
     7321 acaacttcga aggacgccac atcctcttgg tcgaggacaa caggatcaat cagcaggtgg
     7381 cgcttgagct cattcgtaag accggggcca gggtttccct tgcggaaaac ggccgcgagg
     7441 ccctcgcgat ggccgggcgg gagcagttcg acctgattct gatggatatc cagatgccgg
     7501 aaatggacgg actggacgca accatgcaga tacgaagact taatcgggac tggatcgcgc
     7561 aggtgccgat tatcgccatg acggcacacg gaatgtcgca gcacgtcgaa aacagcatac
     7621 gcgcgggaat gaacgcccat ctcaccaagc cggtacaccc ggatcagctt ttcgaaacgc
     7681 ttgcaaggtg gctgaagggg gcaagttcag tggtgaccac tgcggcgccg cggcagtcct
     7741 cgatcgatct tcagccgccg cgattccagc
//
LOCUS       DAOPPA010000093         7714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10577_length_7714_cov_3.842408, whole genome shotgun sequence.
ACCESSION   DAOPPA010000093 DAOPPA010000000
VERSION     DAOPPA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10577_length_7714_cov_3.842408"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3608
                     /locus_tag="PKH54_04220"
     CDS             <1..>3608
                     /locus_tag="PKH54_04220"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99126.1"
                     /translation="PSIASGGQAILVCMPRFVNLCRPCMTGSDCRIGVTATAAMECRE
                     FGAEAGSFCVAPCVDICPEGYDCRESAAGDGKSWCVPEAGTCGCSTLAVIQGAITACS
                     AGVAGIESRCDGVRSCGIDGLSECSAPLPDAERCNGLDDDCDGMTDEAGAIDCANWYF
                     DLDDDGYGLSSVARCLCQAQGTYRASVGGDCDDGNPDIREGAQEQCNEIDDDCDGLTD
                     EPGALRCRTFYPDRDGDTWGDPADGRCLCQPDDTWFLARAGDCDDSSVLVFPGTIDDC
                     NGIDDDCDGVTDPKDAAGCSIWYQDLDSDGYGNAAVSECLCAPSGKFTASKVGDCNDT
                     LATVNPGLTELCNGLDDNCDQVLDPEGSVGCQDWFADRDGDGFGVIWDWQCLCRKNDV
                     YRATEVGDCDDSAKAISPAAQEVCGNSIDENCNGFVSEPGASGCQAWFADSDQDGFGD
                     VGASMCLCGPEAPYTTQVVGDCDDLSGWVSPVSTEVCANEIDDDCDGETDEPGCAGCV
                     QFFLDLDLDGFGVPGNSQCLSQADPASGYVARVGGDCADDNNQVRPDAVERCNGIDDN
                     CDGLTDPDGSVSCIVLYLDFDGDGFGLSSNSRCLCEPEGDYTAVDDGDCDDFNPKVGG
                     GAEVCNGVDDNCNGRIDEENATGCLTWYFDDDGDGVGVTGNSKCLCTASGRYSTRVGG
                     DCDDSSVSVRPGAADTCNGIDDDCDGITDPEGATGCQNWFLDIDGDGYGVTGQGKCLC
                     APGNGYVTRKDGDCNDTVRALNPAAVEFCNGLDDNCDGQTDPAASVGCSAYYADRDFD
                     TWGNSVDTQCLCSAQAPWVVKRGGDCDDSNPLTGPGGVETCNGQDDDCDGLTDEYGAQ
                     GCVNYYLDVDRDGYGVSDSVLCGCKAEGLRDATNGGDCDDTNPAVNPVAAEICNGVDD
                     NCDGQKDQDGAEGCVVYFIDRDSDGFGVAGDSRCLCAPTYPYTSLSVGDCDDTRAAAR
                     PGRTEQCNGFDDDCDGTSDVPGTQGCRTYYLDADADGWAIATDFRCLCAPAAPYLELE
                     PKGDCDDGNPEVNKGKLEVCDGLDNDCNGTTDDDNSEGCLKRYLDADGDGWGIAAQSV
                     CRCQVAAPYTATRIGDCNDGNGAIAPGKLEICNGFDDNCDGITDPESTSGCTTYFRDG
                     DQDGYGINGDSRCLCAGDGYYSTAVGGDCNDDVPTINPGRTEICNNEIDDNCSGAQ"
     assembly_gap    3609..3618
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3619..4036
                     /locus_tag="PKH54_04225"
     CDS             <3619..4036
                     /locus_tag="PKH54_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99127.1"
                     /translation="SGCLWYFRDADTDGYGDESSKRCLCVPDLEYKVRTGGDCCDKDD
                     KVHPGNSDWQTSISACNTWDYNCDGRVIKRWNDANGGCGGWGLFDGCDLHVGWKGSAP
                     NCGESKDWVRDGCGYCGFLWLTCCSEDRENRRQGCN"
     gene            4089..5045
                     /locus_tag="PKH54_04230"
     CDS             4089..5045
                     /locus_tag="PKH54_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC99128.1"
                     /translation="MSGSNGRVLVTGAAGFIGSHLVSRLLDLGFDVLGLDSFCDFYDP
                     AVKRANIATFDNNRSFRLFEADIRDVGAVRDSFGFFRPDIVIHLAAMAGVRPSIENPG
                     LYRDVNVNGTAVILRQAVADGVRRILFASSSSVYGNAATVPFNENLPIDSPLSPYAET
                     KISGERLCVACARQSGIPVTSLRLFTVFGPRQRPDLAISRFLAAVSCGRPIQVFGDGQ
                     SSRDYTYVTDIVDGIVSAIDSGLDVPAINLGNSHPVSLAGLIETVEQVCGRRAIIERL
                     PMQPGDVDRTWADISLAGRVLGFVPRIGILDGVMRQWEWMKG"
     gene            5050..6150
                     /locus_tag="PKH54_04235"
     CDS             5050..6150
                     /locus_tag="PKH54_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOC99129.1"
                     /translation="MNGPAIPRLVVPVSSPAGLAAAVGNGADVVVFNARTAFDSNAAA
                     RDAGFGIVGANAFAEGVERCRRAAVTARVSFVGSFPVRRFADILDMVAAVAEMGVTEV
                     ELSDPGLAAAFCDELPDIKLVFGGGPVANPVAARVIGSAGFSSICFDSSVACSDAVAI
                     SAESGVAAEIPLFGLLPFAWCSSCMAGEYFDGQSCNGSAFGPCRRVFTTEGGRSAGRL
                     LEMGFSGGLLMLGDLVRAGIPSVRVLVSGRRASVVADVTAVFREAIDAATRALVDGGV
                     DALKPSMYPVHALHFWNGRLVSAALFGDIVPSGMRANPCPGRAGGTVRCGPLSRVARF
                     VEPMAVRMESSTRGGFGDRRENWDTDFDWEDA"
     gene            6152..6943
                     /locus_tag="PKH54_04240"
     CDS             6152..6943
                     /locus_tag="PKH54_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99130.1"
                     /translation="MDGRPAERTVLRQPDQRIRTVAIHVTGDPEPFLKCGADRYWVGV
                     RGSVSEFLELASDGTIASWLSAAGRTRRKVGLVTPYCVDAARFDQVISAIADFSVGVD
                     RVMTGDPGVASRISDAVRTYLVAAVHNVDHAIFLRRLGIHGVRAIRPVSTGAMDVNRE
                     IDLEMPVFGRLPIAFMPECDRGSVGDAPVELHGRGGGIILCRGYAVTSDYLDLSDSLR
                     PFGRDTVGVVETFGLTPDEVAEAVDAIRFGRPVETNRPLFVSEQD"
     gene            7144..>7714
                     /locus_tag="PKH54_04245"
     CDS             7144..>7714
                     /locus_tag="PKH54_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOC99131.1"
                     /translation="MKHDSIAVIDFGGQYAHLIATKVRRAGVLAEIRQPDDPTDMFRG
                     YKGVILSGSPSLSAFGEDSEWNKEVFELDIPILGFCFGHQEIAKYYGGRVEHGGREWG
                     LATLNILVDHPIFKGLQRKETVWMSHFDTVVAIGPDFQEYGYSKLGETGKEHRLAAIG
                     SDKYRRYGFQFHPEVENSVHGDEMIANFVI"
BASE COUNT         1389 a         2131 c         2522 g         1662 t
ORIGIN      
        1 ttccttcaat cgcttcgggt ggccaggcga tactggtgtg catgcccaga ttcgtcaatc
       61 tttgccgccc ctgcatgaca ggttccgatt gcaggatcgg cgttacggcc acggcggcca
      121 tggaatgccg tgaatttggg gccgaggccg gaagtttctg cgtcgccccg tgcgtcgata
      181 tttgtccgga aggttatgac tgccgtgagt ccgcggccgg cgacggcaag tcgtggtgcg
      241 ttccggaagc cggaacctgc ggatgttcca cccttgccgt gattcagggc gccatcacgg
      301 cctgcagcgc cggtgtcgcg ggaatcgagt ccaggtgtga cggcgtcaga tcctgcggca
      361 ttgacggact gagcgaatgc agcgccccgc tgcccgatgc cgaaaggtgc aacggactgg
      421 atgacgactg cgacggcatg acggatgagg ccggcgccat cgattgtgcc aactggtatt
      481 tcgaccttga tgacgacgga tatggccttt cgtcggtcgc caggtgcctt tgccaggccc
      541 agggcactta tcgggcaagc gtcggcggcg attgcgacga cggcaatccc gacatcaggg
      601 aaggcgccca ggagcagtgc aacgaaattg acgatgattg tgacggattg accgacgaac
      661 cgggcgctct tcgatgccgg acattctatc cggatcggga cggggacacg tggggtgatc
      721 cggccgatgg gcgctgcctt tgccagcccg acgacacatg gttccttgcc agggccggcg
      781 actgcgatga ctccagtgtg ctggtgttcc ccggaaccat tgatgattgc aatggaatcg
      841 atgacgactg cgatggcgtg acggatccga aggatgcggc cggatgttcc atctggtatc
      901 aggatctgga ctcggacggt tacggcaacg ccgccgtctc ggaatgtctc tgcgccccgt
      961 cgggcaagtt taccgccagc aaggtcggtg attgcaacga cacgctggcc actgtcaatc
     1021 ccgggttgac cgaactgtgc aacggcctgg acgacaattg cgatcaggtg cttgatccgg
     1081 aaggttccgt cggttgccag gactggttcg cggatcgtga tggcgacggt ttcggcgtga
     1141 tatgggactg gcagtgcctt tgtcgaaaga acgatgtcta tcgggcgacc gaagttggcg
     1201 actgcgatga ttccgcaaag gcgatttccc cggcggccca ggaagtctgc gggaattcga
     1261 tcgacgaaaa ctgcaatgga ttcgtcagcg agcctggcgc atcggggtgc caggcgtggt
     1321 ttgccgacag cgatcaggac ggttttggtg atgtcggtgc gtccatgtgc ctttgtggac
     1381 cggaggcgcc atatacgaca caggtggtcg gcgattgcga cgacctttcc gggtgggtgt
     1441 cgccggtttc aactgaagtg tgcgcgaacg aaatcgacga cgactgcgat ggggaaaccg
     1501 acgagcccgg gtgtgccgga tgcgtccagt ttttcctgga tttggatctt gatggattcg
     1561 gcgtgcctgg caattcgcag tgcctgtccc aggccgatcc ggcttccggc tatgttgcca
     1621 gggtcggcgg cgactgcgcg gacgacaata accaggtgcg tcccgatgcc gttgaacgtt
     1681 gcaacggcat tgacgacaac tgcgacggac tgaccgaccc ggatggttcg gtttcgtgca
     1741 ttgttcttta tctggatttc gacggcgacg ggttcgggct gtcctccaac agcaggtgcc
     1801 tttgcgaacc cgagggcgac tacaccgcag tcgatgacgg ggattgcgac gatttcaacc
     1861 cgaaggtcgg tggcggggcg gaagtctgca acggcgtcga tgacaactgc aacggacgaa
     1921 tcgatgaaga gaacgcgaca ggctgcctga cgtggtattt cgacgacgat ggggacgggg
     1981 tcggggtcac cggaaattcg aagtgcctct gcacggcgtc cgggcggtat tcgacacgcg
     2041 tgggcggcga ttgcgacgat tcaagcgttt ccgtgcgtcc tggcgccgcg gatacctgca
     2101 atggaatcga tgacgattgc gacggaatta ccgatcccga gggagcgacc ggttgccaga
     2161 actggttcct cgatatcgac ggcgatggat acggcgtgac ggggcagggc aagtgccttt
     2221 gcgctcccgg gaacggctat gtcacaagga aggacggcga ctgcaacgac accgtgcgcg
     2281 ctttgaatcc cgccgcggtg gaattctgca acgggcttga cgacaactgc gatggccaga
     2341 cggatccggc cgcttcggtc ggttgttcgg cgtactacgc ggatcgcgac ttcgatacat
     2401 ggggcaattc cgttgacacg cagtgcctct gttcggccca ggccccttgg gttgtcaagc
     2461 gcggcgggga ctgcgacgac tccaatccgt tgaccggccc tggcggggtt gaaacctgca
     2521 atggtcagga cgacgactgc gacggtttga cggacgaata cggggcgcaa ggctgcgtga
     2581 attattacct cgacgtcgat cgcgacggat atggcgtttc cgattccgtt ctttgcggct
     2641 gcaaggcaga ggggcttcgc gatgccacca atggcggtga ctgcgacgac actaacccgg
     2701 cggtcaatcc tgttgccgcc gagatctgca atggcgtcga tgacaactgt gacggccaga
     2761 aggatcagga cggtgcggag ggctgcgtcg tgtatttcat cgatcgcgat tcggacggct
     2821 ttggcgtggc cggcgattca cggtgcctgt gcgcgccgac ttacccgtac acgtcgcttt
     2881 cggttggcga ttgcgatgat accagggccg ccgccaggcc gggccgaacc gagcagtgca
     2941 atggattcga cgacgattgc gatggaacgt cggatgttcc cggcactcag ggttgcagga
     3001 cgtactacct ggacgcggat gcggacggat gggcgattgc gaccgatttc cgttgccttt
     3061 gcgcccccgc ggccccgtat cttgaacttg agccgaaggg cgattgcgac gacggaaatc
     3121 ctgaagtcaa caagggtaag cttgaagtct gtgacggcct ggacaacgat tgcaacggca
     3181 cgacggacga cgacaattcg gaaggatgcc tgaagcgcta tcttgacgcc gatggcgacg
     3241 gatggggtat cgcggcccag tcggtttgtc gctgccaggt tgcggcacca tacactgcga
     3301 ccaggattgg ggattgtaac gacggcaacg gtgcgatcgc ccccggtaag ctcgaaatct
     3361 gcaacggttt tgatgacaac tgcgacggca tcaccgatcc cgaatcgact tcaggttgta
     3421 cgacgtactt ccgcgacggc gatcaggacg gatatggaat caacggcgat tccaggtgcc
     3481 tctgcgcggg ggatggatac tattccacgg cggtcggtgg cgactgcaat gacgacgttc
     3541 cgacaatcaa cccgggacgc accgagatct gcaacaacga gattgacgac aactgctctg
     3601 gtgcacagnn nnnnnnnnat ccggctgcct ctggtatttc agggacgccg acaccgatgg
     3661 gtacggcgac gaatcttcaa agcgttgcct gtgcgtcccc gacctggaat acaaggtcag
     3721 gaccggtggc gactgctgcg acaaggacga caaggttcac cccggaaaca gtgactggca
     3781 gacgtccatc agtgcgtgca acacgtggga ctacaactgc gacggtcggg ttatcaagcg
     3841 ctggaacgac gccaacggcg gttgcggcgg ctggggcctg ttcgacggct gcgatctgca
     3901 tgttggctgg aagggctcgg cgccgaactg tggcgagtcg aaggactggg ttcgggatgg
     3961 gtgcggatat tgcgggttcc tttggctgac ctgttgctcc gaggacaggg aaaacaggcg
     4021 tcagggttgc aattagcggc gtctcgatgg catattttgc ccggtaacag ggctttcggg
     4081 gattgctgat gtcaggaagc aacggccggg tgctggtcac cggtgccgcg ggattcattg
     4141 gttcgcacct ggtttcaagg ctgctggatc tgggcttcga tgtcctgggg ctggacagct
     4201 tctgtgattt ttacgatccc gcggtcaagc gggccaatat cgcgacattc gataataatc
     4261 ggtcatttcg tcttttcgag gccgacatcc gcgacgtggg cgcggttcgg gattcgttcg
     4321 gcttctttcg tccggacatc gtcattcatc tggctgcgat ggcgggcgtg cggcccagca
     4381 ttgaaaaccc cggcctttat cgtgacgtca atgtgaacgg aaccgccgtg atattgcgtc
     4441 aggccgtggc tgacggtgtt cggcgcatcc tgttcgcttc aagttcgtcc gtatatggga
     4501 atgccgcgac ggttcctttt aacgagaacc tgccaatcga ttctccgtta agtccatatg
     4561 ctgaaacaaa aatctccggc gagcgtcttt gcgtggcctg cgctcggcag tcggggattc
     4621 cggttaccag tcttcgtctt ttcacggtct ttggaccacg gcagcgtccc gatctggcca
     4681 tcagccgttt tcttgccgcc gtttcctgtg gtcgtccgat ccaggtcttc ggtgatgggc
     4741 agtcttccag ggattacaca tatgttaccg atatcgttga cggaatcgtt tcggcaatcg
     4801 attcgggcct ggatgtgccg gccatcaatc tgggcaattc acatccggtt tcactggccg
     4861 gacttattga aaccgttgaa caagtctgcg gacgtcgggc aattatagaa agacttccga
     4921 tgcagcctgg cgatgtcgat cgtacctggg ccgacatttc ccttgccggc cgtgtgctcg
     4981 ggttcgtgcc gcggattggg attctggatg gtgtcatgcg acagtgggag tggatgaagg
     5041 gttgaagggt tgaacgggcc agcaattccc agacttgtcg tcccggtgtc cagtccggcc
     5101 ggtcttgccg cggccgtcgg caatggcgcc gacgtggtcg tcttcaacgc aaggacggca
     5161 ttcgattcga atgcggctgc gcgtgatgcc ggattcggca ttgtgggtgc caatgccttt
     5221 gccgaggggg tcgagcggtg ccgtcgcgcc gcggttaccg cccgcgtgtc gtttgtgggc
     5281 agctttcccg ttcgtcgttt cgccgatatt ctggacatgg tggctgccgt tgccgagatg
     5341 ggtgtcaccg aggtcgaact ttcagatccg gggcttgccg cagctttctg cgatgaactt
     5401 cccgatatca aacttgtttt cggcggcggc cccgtggcca atccggttgc ggcgcgggtt
     5461 atcgggtcgg ctggattttc ttcaatatgc ttcgattctt ctgtcgcatg ctccgatgcg
     5521 gtcgccattt ccgctgaatc cggcgtcgct gccgagattc ccttgttcgg gctgcttccg
     5581 ttcgcctggt gcagttcgtg catggctggg gagtactttg acgggcagtc ttgcaacggc
     5641 agtgcgttcg ggccttgcag gcgggtattc accaccgaag gcggcagaag tgccggacgt
     5701 cttctggaaa tgggattttc cggtggcctg ttgatgcttg gggatcttgt cagggccggg
     5761 atcccatcgg tccgggttct ggtttccggt cgtcgcgcat ccgttgttgc cgacgtgacg
     5821 gccgtgtttc gcgaggcaat cgacgcggcg acgagggcac tggtcgatgg tggcgtcgat
     5881 gccttgaagc cgtcgatgta tcctgtacat gcgctgcact tctggaacgg caggctggtt
     5941 tcagccgcgc tgtttgggga catagtgccg tccggcatgc gcgcaaatcc gtgtccgggg
     6001 cgggcgggcg gaaccgtccg ttgcgggccg ctttccaggg tcgcccggtt tgtcgagcct
     6061 atggctgttc gaatggaatc atcgacgcgg ggcggtttcg gcgacaggcg ggaaaactgg
     6121 gacacggact ttgactggga ggatgcctga gatggacggc cgaccggcag aaagaaccgt
     6181 cttgcgtcag cccgatcagc ggattcgaac cgttgcgata catgttaccg gggatccgga
     6241 acctttcctg aagtgcgggg cggatcgata ctgggttggg gttcgcggct cggtgtctga
     6301 gttcctggaa ctggcgtccg acggaaccat cgcttcctgg ctctctgccg cggggcgtac
     6361 caggcgaaag gtcggtcttg ttaccccgta ctgcgttgat gctgccaggt ttgatcaggt
     6421 catttccgcc atcgccgact tttcggtcgg cgtcgatcgc gtaatgaccg gggatcccgg
     6481 tgttgcttca aggatttccg atgccgtaag gacttacctg gttgcagccg tccataatgt
     6541 cgatcacgcc atattcctgc gcaggctcgg gattcacggt gtcagggcca ttcgtccggt
     6601 atcgacaggc gcgatggacg tcaaccggga aatcgatctt gaaatgccgg tgttcgggcg
     6661 gctgccgatt gccttcatgc cggaatgcga tcgcggcagc gtcggggatg ccccggtcga
     6721 acttcatggc cgcggcggtg gaatcatcct ttgccggggc tacgcggtga catccgacta
     6781 tctggatctg tccgacagcc tgcgtccgtt cggtcgcgac acggtcgggg tcgtcgagac
     6841 gttcgggctg accccagacg aagtcgccga ggcggtcgac gcgattcgat tcggtcggcc
     6901 cgtcgaaacc aatcgcccgc ttttcgtatc cgaacaggac tgaccttcct tgtacgggcg
     6961 gattacatcc gcccgccgcg gtcaagcaag atgctatcca tcgaggccgc ccgttaatat
     7021 caacagcttt ttttcctggg tggcctggac ccggatacga atttgcttca aacgccttac
     7081 aatacgccat cccggccaat cgcggccggt gcactgattt gccaataata ccgggggttc
     7141 ccgatgaaac atgactccat cgcagtaatc gatttcggcg gacagtacgc gcatcttatc
     7201 gcgaccaagg tccgccgcgc cggcgtcctg gccgagatcc gccagcccga cgacccgacc
     7261 gacatgttcc gcggatacaa gggtgtcatc ctgtccggca gtccgtcgct ttccgcgttc
     7321 ggcgaggaca gcgaatggaa caaggaagtc tttgaactgg acatcccgat cctgggcttc
     7381 tgcttcggac accaggaaat cgcgaagtac tacggcggcc gtgtggagca cggcggacgc
     7441 gagtggggcc tggcgaccct gaatatcctg gtcgatcacc cgatcttcaa ggggctgcag
     7501 cgcaaggaaa cggtctggat gtcccacttc gacaccgtcg tggccatcgg tccggacttc
     7561 caggaatacg gttacagcaa gctgggcgag accggcaagg aacaccgcct ggccgccatc
     7621 ggttcggaca agtatcgccg ctatggcttc cagttccacc ctgaagtcga aaacagcgtt
     7681 cacggcgacg agatgatcgc caacttcgtc atcg
//
LOCUS       DAOPPA010000094         7656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10676_length_7656_cov_3.993553, whole genome shotgun sequence.
ACCESSION   DAOPPA010000094 DAOPPA010000000
VERSION     DAOPPA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10676_length_7656_cov_3.993553"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="PKH54_04250"
     CDS             complement(<1..2156)
                     /locus_tag="PKH54_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99132.1"
                     /translation="MPVFVREFNVLALLLPVLVSVGCASGSSPIFDVIPDETSNEASV
                     VDSSECQVDCDVDVAEDSGSRLDEGGFDSAGDLVVGEDIAADTQSDVWHDVSDLDGLD
                     AAADLPEDTTGDLKADTSSDDADINSDTAFDDVVDVTPDGVLSVDIDADEDVLVDFGA
                     EVTDPDDGSDVVIDAEDVVEPPICLVHSDCDFNFRCVTDVHPSYCAWASTCDDKDDCS
                     TFQTCRQVDNWKECRTGVNTCTRDGDCPEDMYCADKVLGVGSCKTRNECYEDENCPPY
                     HTCGTNHVWRICVPDPDECATDSDCALHFQCVTNGVHLECRPRPDECAIATDCPQYFV
                     CTSNGVYKECVPDSDKCAFDDECPRYFTCSSNGVFMDCLPLPDECSADADCGDDYLCG
                     SNGVYRICFRAPDQCLTADDCGFGFACVQTWARRWVCRDVNQCDSDEECLGTTVCTNV
                     GNWNECLPPVDECGSHADCAFNYRCDSGGLVNACVWASTCHVNGDCNFAYSCQQVNNW
                     KECRFGADSCSSDSDCSPDMYCRKVVGSLGTCDTRDQCSTTADCAEGSECRFNGLNNV
                     CVELPDECAVASDCPEDYYCRIGSPRNTCALKADECRVDFDCRYGMSCEQNPSARLVC
                     RNANECLLNADCPAGQRCVPGMQWYVCEIPTDECAVNSDCNFNYRCNIAVNPTVCAWA
                     STCSSNDDCGFGQICRQVENWKECRVGVNVCSRDAD"
     gene            complement(2269..2781)
                     /locus_tag="PKH54_04255"
     CDS             complement(2269..2781)
                     /locus_tag="PKH54_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99133.1"
                     /translation="MLMVFMAFINFTRMKVYAATRWLWLALSGGLFVTAPIGCEEEDP
                     QPLYGVPEDIQVLDSAEEDIGPQPEYGPGYDVIVPDAEPQDFQTLYGPPPDFIQGDEG
                     SVDQGIQPLYGAVEIIDDYVPPQELPTDDLAQPLYGALEVFDDVAEKDGAASDVESDL
                     PQTLYGPAAK"
     gene            complement(2917..4014)
                     /locus_tag="PKH54_04260"
     CDS             complement(2917..4014)
                     /locus_tag="PKH54_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99134.1"
                     /translation="MNRVAKKRFNLWAWRHFVDFEFAHPRLTYLFWEATLDCNMACRH
                     CGSDCRRSTDISTELKADEVLRVFSEIARTHNPRRVFVAVTGGEPLVRPDLFDVMAQV
                     RRMGYPWGMVSNGWAVDDRVVDQARRSGMRTLVISLDGVNAESHDWLRGRGSFDRAVD
                     AIGRFRRAGFLRNLQVTSTFHRRNIDELEPMYRLMIDLGIADWRVVSVFPNGRAARQA
                     DFLLEPSELRKMMDFIAEKRSRPRPIKVSYGDEGYLGCQYERDVRDFFYACFSGVRIA
                     SILADGGVTGCPNNPRTLVQGNVRDRSFMDIWTNEFGKFRDRRWMRQGDCARCRDFAI
                     CKGNSMHLWDTEKSGPKVCHVRMLKDAGARV"
     gene            complement(4064..5587)
                     /locus_tag="PKH54_04265"
     CDS             complement(4064..5587)
                     /locus_tag="PKH54_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99135.1"
                     /translation="MGVDPALAAASILLVVAGATGWPRGRSGRVVLVLLCLAVILLSA
                     LPVFTVPFSHDAQILRVAFAARDASADWNHPFLSYLLNRPAALHSFDPVILRLVPFLW
                     FAAEALLSGLVAYRLAGRLAAALTVIWMAHSVRLSMGMVDLSDWNLAGVFLSLMLLRA
                     TRGGPSSGLGRYVPAGALLILGGCFSSYMMIVPSLVMLAWEIRRSATGLQSFWHAAAS
                     AVAAAVALYFFLDVVSIGRMAPKLGFAADFLPVVSEMFLVSPPLGQGPLIPALTLGGL
                     VLMPGSGRRSSWLFLAATVLLGLIAVLAGLVLAAINGGYYFGLMKGPVFIAAAAFTAA
                     ASRFPAGQLCRTGLSRRAASALAALVVLSVVVAGTFRTAPIDEVMKASGVEHVGGLVD
                     ASGRGERRILSNDQNAVILMEYHEIVEGRMDIGQILDSRRSNWVTRTVSVFGVDSIDC
                     KSLPADFLFMFRRSGDRMKECDLFRAADCRELFADGGGGECRESGRTFCYYDCRRKD"
     gene            5689..>7656
                     /locus_tag="PKH54_04270"
     CDS             5689..>7656
                     /locus_tag="PKH54_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOC99136.1"
                     /translation="MTVMPMDGKSRIAPCLMAFAMASVLSVMVVHPASARAPSAIVYG
                     ADEDFPPFEWVAPDGTLQGFNIDLMRGISSITGMTIQFVPGPWSEIRQKQWEGSIDVI
                     SMFKSQDREAALAFSDPLTVGYDTIWIRKGEDDVKNLDQLAGKGVLVQQTGFAAEFLS
                     GSIPSARLIFVESEPQALVALENGRGDVAIVSELTARTAIGKLGLKAIRKTEPPILPR
                     HLAFATTFDKTPVLDELNAAMAMMRADGRYSELEAAWLEAPAISGPVQEFLKKYGRLT
                     LLIILGLVTWLAAWTIILRRRVAARTKALQGELQERRRAEEELRRSQEVFDVLFNFNP
                     VMMSLSSLNEGRYLEVNPAAVKLLGFSRLEMIGRTSSELNLWVDPGDRTKIVRQVLEN
                     GHCRNYPLSIRERAGRHIECILNMEKVVIRGEDVVLTTIHDVSNLRSAEREKARLESQ
                     LRQIQKIEMAGRLAGGVAHDFNNQLTIIRGYCDVLSRRLSSAADISVLEEIRKAVNHS
                     SSLTSSLLAFSRRQVRRLEILDPGTLIREMEQSLRVSLGENLNLDIAISDTVDRIEMD
                     RGQFVEVIYNIVANARDAMGTGGTLAISVNGMSVTDSLNVGGVPVACGDYVVITLADD
                     GIGMSDDTMKLLFEPFFTTKEVGKGIGLGLST"
BASE COUNT         1757 a         2333 c         2134 g         1432 t
ORIGIN      
        1 caatcggcat cccttgaaca cacgttgaca ccgaccctgc attccttcca gttctcgacc
       61 tgtctgcata tctggccgaa accacaatca tcgttgctgg aacacgtgct ggcccacgcg
      121 cagacggtcg gattcacggc gatattgcag cggtaattga agttgcaatc cgagttgacc
      181 gcgcactcgt cggtcggaat ctcgcagacg taccactgca tgccgggaac gcaccgttga
      241 ccggcgggac aatcagcgtt aagcaggcac tcgtttgcat ttctgcacac cagcctggca
      301 cttggattct gttcgcatga catcccgtac cgacagtcaa aatccacccg gcactcgtcg
      361 gccttcagtg cgcatgtatt tctgggggag cctatccggc agtaatagtc ttccggacaa
      421 tccgaggcga cggcacactc gtcaggcaat tccacgcaga cattgttgag gccgttgaac
      481 ctgcattcgg aaccctccgc acaatcagcc gtggttgaac actgatcacg cgtgtcacaa
      541 gttccaaggc ttccaacaac cttcctgcaa tacatgtcag gggagcaatc ggaatccgag
      601 gaacacgagt cggcaccgaa cctgcattcc ttccagttat tcacctgctg gcacgaatat
      661 gcgaaattgc agtcgccgtt gacgtgacat gtcgatgccc agacacacgc attgaccaag
      721 ccaccgctgt cgcacctgta attgaaggca cagtctgcgt ggctcccgca ctcgtcaacc
      781 ggcggcaggc actcgttcca gttgccgacg ttggtgcaca cggtcgttcc aaggcattct
      841 tcatctgagt cacactggtt cacgtcacgg cacacccagc ggcgagccca cgtctggaca
      901 caggcaaatc cgaaaccgca atcatcggcg gtcaagcact gatccggtgc cctgaagcaa
      961 atccgataca ctccgttgct tccacataga tagtcgtcgc cgcaatcggc gtcggcagaa
     1021 cactcgtccg ggagaggaag acaatccatg aacactccgt tggacgaaca tgtgaaataa
     1081 cgcgggcatt catcatcgaa cgcacacttg tcggaatccg ggacgcattc tttgtacaca
     1141 ccgttcgatg tgcagacaaa gtactgtgga caatcggtcg caatggcgca ttcatcgggc
     1201 ctgggacggc attcaaggtg aacgccgttc gtcacgcact gaaaatgaag cgcacaatcg
     1261 gaatcggtcg cacactcgtc gggatccggc acgcagatgc gccagacatg gttggtgccg
     1321 catgtatggt acggcggaca gttttcgtcc tcgtagcact cgttcctggt cttgcaggaa
     1381 ccgacgccaa gaaccttgtc ggcacaatac atgtcttcgg ggcaatcgcc gtccctggtg
     1441 cacgtattca ctccggttct gcactccttc cagttatcga cctgccggca tgtctggaaa
     1501 gttgaacagt cgtccttgtc gtcacaggta ctcgcccatg cacaataaga aggatgaacg
     1561 tcggtgacgc acctgaaatt gaagtcgcaa tcggaatgaa ccagacaaat cggcggctcg
     1621 acaacgtctt ctgcatcaat cacgacatcg gatccgtcat cgggatcggt cacttcggct
     1681 ccgaaatcaa ccaaaacatc ttcatctgca tcgatatcga cactcaggac accatccggc
     1741 gtgacgtcga cgacgtcatc aaatgcggtg tcggaattga tgtcggcgtc atcgcttgaa
     1801 gtatccgcct tcaaatcgcc tgtggtgtcc tccggaagat cggccgcggc atccaggccg
     1861 tccagatccg aaacgtcgtg ccagacgtcg gactgagtat cagccgcgat atcctcaccg
     1921 acgaccaggt cacccgctga atcaaacccg ccctcgtcaa gcctggaacc tgagtcctcg
     1981 gcaacatcga cgtcgcaatc gacctgacat tcggatgaat caactactga agcttcgttt
     2041 gatgtttcat cgggaatcac atcgaatatc ggggacgagc ccgaggcgca tccgacggaa
     2101 accagaaccg gcagcaggag tgccaggaca ttgaattcac gaacaaatac aggcatggga
     2161 ttaaactccg tgtaaaacgg ccccggatgc ggggtcataa aatatggcgt aatctacaaa
     2221 atgagcccgg tcgaccgcaa gacctgatgc ggtaatacct gtcggaagtt acttcgcggc
     2281 gggaccgtag agcgtctgag gaagatcgga ctcgacatcg gatgcggcgc catccttttc
     2341 cgcgacgtcg tcgaacacct cgagcgcccc gtaaagcggc tgggccaggt catccgtggg
     2401 cagttcctgg ggcggcacat agtcatcaat gatttcgaca gccccataga gcggctggat
     2461 accctgatcg accgaaccct cgtccccctg gatgaaatcc ggcggcgggc cgtacagagt
     2521 ctggaaatcc tgcggttcgg cgtcgggaac gatcacatcg taaccggggc cgtactccgg
     2581 ctggggtccg atgtcctctt cagccgaatc aagaacctgg atgtcttcgg gaacgccgta
     2641 aaggggttgc gggtcttctt cctcgcaacc gatcggcgcg gtcacgaaca ggccaccgga
     2701 aagggccagc caaagccatc gcgtggccgc ataaaccttc atccttgtga agttgatgaa
     2761 agccatgaat accatcagca tgataaaccc ccgaaacagt gcggcaatta tagcaaagga
     2821 tcaataagca cttcaatatc cgggcgggcg gatgaaccga cttcgcgcaa actcgggcgt
     2881 gccgatggac tcgctcccaa caacaagacg gtcggcctat acccgcgccc cggcatcctt
     2941 cagcatccgc acgtggcaca ccttcggacc gctcttttcg gtgtcccaca ggtgcatgct
     3001 gttgcccttg catatggcga agtcgcggca tctggcgcaa tcaccctggc gcatccacct
     3061 tcgatcccgg aacttgccga attcattcgt ccatatatcc atgaacgacc gatcgcggac
     3121 gttcccctgg acaagggttc tcgggttgtt ggggcagccc gtcacaccgc cgtcggcaag
     3181 tatcgacgca atcctgacgc cagagaaaca ggcataaaag aaatccctga cgtcacgctc
     3241 gtactggcaa cccagatatc cttcgtcgcc ataggaaacc ttgatcggac gcggccttga
     3301 tctcttttcg gcgatgaagt ccatcatctt ccggagctcg gatggttcaa gcaggaagtc
     3361 ggcctgcctt gccgcgcggc cgtttggaaa taccgacacc acgcgccagt cggcgatgcc
     3421 aagatcaatc atcaggcgat acatcggctc gagttcatcg atatttcggc gatgaaatgt
     3481 cgaggtgacc tgaagattgc gcaggaagcc ggcccgacgg aaacgaccga tcgcgtcaac
     3541 cgcccgatcg aaggaacccc gcccgcgaag ccagtcgtga gattcggcgt tgaccccgtc
     3601 cagactgata acaagtgtcc gcatgccgct tcgcctggcc tgatccacga cccggtcatc
     3661 aacggcccag ccgttggata ccatccccca cggatatccc atgcgcctga cctgagccat
     3721 aacgtcgaaa aggtccggcc ttaccagggg ttccccaccc gtcacggcca cgaataccct
     3781 ccggggatta tgcgtcctgg cgatttcgga aaacacccgc aggacttcat cggccttcaa
     3841 ttccgtgctg atgtcagtgg atctgcggca gtcggatccg cagtgcctgc aggccatatt
     3901 gcagtcaagc gtcgcctccc agaacaggta ggtcagccgc gggtgcgcga actcgaaatc
     3961 gacgaaatga cgccaggccc agaggttgaa acgcttcttt gcaaccctgt tcatcaatca
     4021 agcctcaaat caaacggaac cccgcgcgaa accgcgcctc gcctcaatct ttccgacggc
     4081 agtcataata gcagaatgtt cttccggatt cccggcattc accgccgccg ccgtccgcga
     4141 acagttccct gcagtcggct gcccgaaaca ggtcgcattc cttcatccga tcaccggaac
     4201 gcctgaacat gaaaagaaaa tctgccggaa gggatttgca gtcaatgctg tcaacaccaa
     4261 aaacggacac ggtgcgcgtt acccagttcg aacgccttga atcgaggatc tggccgatat
     4321 ccatcctgcc ttcgacaatc tcgtgatatt ccatcaggat caccgcattc tgatcgtttg
     4381 acaggatcct tcgctcgccg cgtcccgagg catcgaccag cccgccgacg tgctcgacgc
     4441 cggacgcctt catgacttcg tcaatcgggg cggtccggaa agtacccgcg acgacgaccg
     4501 acaggacgac cagggccgcg agtgctgacg cggcccgccg ggataatccc gtcctgcata
     4561 actgcccggc cgggaacctg gacgccgcgg ccgtgaaggc tgcggccgcg atgaagaccg
     4621 gtcccttcat gaggccgaag tagtagccac cgtttattgc cgccagcaca aggccggcca
     4681 ggaccgcgat cagccccaga aggacggtcg ccgcaaggaa cagccacgag gatctgcgac
     4741 cggaacccgg catcaggaca agcccgccca gggtcagggc agggatcagg gggccctggc
     4801 cgagcggcgg ggacacaagg aacatctccg aaaccaccgg aaggaagtcc gcggcgaatc
     4861 ccagtttcgg ggccatcctt ccaatcgaca cgacgtcgag gaagaaatag agagccaccg
     4921 ccgcagcgac agcggacgcc gcagcatgcc agaaggactg aagcccggtc gccgaccgac
     4981 ggatctccca cgcaagcata accagcgacg gaacgatcat catatacgac gaaaaacatc
     5041 cgcccaggat cagcagcgct ccggcaggaa cgtaccggcc cagacctgaa gacggcccgc
     5101 ccctggtcgc acgcaacagc atcagcgaaa ggaagacccc ggccaggttc caatcagaca
     5161 ggtcaaccat ccccattgaa agccggacgg aatgggccat ccatattacc gtcagtgccg
     5221 ctgccaggcg gccggcaagc ctgtacgcga caagtcccga aagcagagcc tcggccgcga
     5281 accacaggaa tggcaccagc cgcaggatga ccgggtcaaa cgaatgcaac gcggcggggc
     5341 ggttcagaag gtacgacaga aacggatgat tccagtcggc gcttgcgtcc cgcgcggcaa
     5401 aggccacgcg caggatctgg gcgtcgtgtg aaaatggaac cgtgaacacc ggcagtgccg
     5461 aaagaaggat gaccgctagg cagagcagaa ccaggacgac gcgcccggaa cgtccgcgcg
     5521 gccagccggt cgcgcccgcg acaacaagaa gtatcgaagc ggcagccaga gccggatcca
     5581 cacccaaggc agacaccccg taaacagtca aatcaaggta ttactaatcc atccgcttac
     5641 gcaaccgata ttctgtagta acataccgtt tcaagtcacg cattgaagat gactgtcatg
     5701 ccgatggacg ggaaatcaag gattgccccc tgtttgatgg cattcgccat ggcatccgtc
     5761 ctgtccgtca tggtcgtgca tccggcgtcc gcgcgcgcgc cgtccgcgat tgtctatggc
     5821 gccgacgagg actttccccc gttcgagtgg gtggcgccgg acggcacgct gcagggattc
     5881 aacatcgatc tgatgcgtgg catcagcagt atcaccggca tgacgattca gttcgtgcca
     5941 ggcccctggt cggaaatccg ccagaaacaa tgggaagggt ctattgacgt catctcgatg
     6001 ttcaagtccc aggatcgcga ggcggcactc gccttttccg atcccctgac ggtcggttac
     6061 gacacgatct ggattcgaaa gggcgaagat gatgtaaaga acctggatca gcttgcgggc
     6121 aagggtgtcc tggttcagca gaccggcttc gccgccgagt tcctttcggg ctcgattcct
     6181 tcggcaagac tgatctttgt cgagtcggag ccacaggctc ttgtcgccct tgaaaacggt
     6241 cgcggcgatg tggcgatcgt gtcggagctt accgccagga ccgccatcgg caagctcggc
     6301 ctgaaggcta tcaggaagac cgaaccgccg attctgcccc gccatcttgc cttcgcgacg
     6361 actttcgaca agacccctgt cctggacgaa ctcaacgccg caatggcaat gatgcgcgcg
     6421 gatggcaggt attcagagct ggaggctgcg tggctggaag caccggccat ctccggcccg
     6481 gttcaggaat tcctgaaaaa atacggcaga ttgaccctgc ttatcattct tggcctggtc
     6541 acatggctgg ccgcgtggac aatcatcctt cgaaggcgcg tggccgcccg gacaaaggcg
     6601 cttcaggggg aacttcagga acgtcggcga gccgaggagg agcttcgcag gtcgcaggag
     6661 gtgttcgacg tcctgttcaa cttcaacccg gtgatgatgt ccctttcgtc cttgaatgaa
     6721 ggtcgctacc tggaagtgaa ccccgcggcc gttaagttgc ttggcttctc acgactggaa
     6781 atgattggca ggacttcgag cgagctcaac ctgtgggtgg atcccgggga tcgcacgaag
     6841 atcgtgcgcc aggtgcttga aaacgggcac tgtcgaaatt atccactgtc gataagggaa
     6901 agggccgggc ggcatatcga gtgcatcctg aacatggaaa aggtggtcat ccggggcgag
     6961 gacgtcgtcc ttaccaccat tcacgacgtt tcaaacctca ggtctgccga acgcgagaag
     7021 gctcgcctcg agagccagtt gcgtcaaatc cagaagattg agatggcggg caggcttgcg
     7081 ggtggggtcg cgcacgactt caacaaccag ctcacgataa tccgcggata ctgcgacgta
     7141 ctttcacgca ggctgtcatc ggcagcggac ataagcgtgc tcgaggagat tcgcaaggcg
     7201 gtcaaccact cgtcgtcgct caccagcagt cttcttgctt tctcgcgcag gcaggttcgc
     7261 cggctggaga tcctggatcc cggcacactg atccgggaaa tggaacagtc gctgcgtgtt
     7321 tcccttggcg aaaaccttaa cctggatatc gcgatttccg acacggttga caggattgag
     7381 atggatcgcg gtcagttcgt cgaagtcatt tacaacatcg ttgccaacgc cagggacgcc
     7441 atgggaaccg gcggcacgct tgcaatcagc gttaacggca tgtcggtaac cgattcgctc
     7501 aatgttgggg gcgtcccggt ggcgtgcggc gactatgtcg tgatcacact tgccgacgac
     7561 gggatcggca tgtccgacga cacgatgaaa ctgcttttcg agccgttctt caccacgaag
     7621 gaggtcggaa aaggcatcgg gctcggactt tcgacc
//
LOCUS       DAOPPA010000095         7638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10702_length_7638_cov_3.952525, whole genome shotgun sequence.
ACCESSION   DAOPPA010000095 DAOPPA010000000
VERSION     DAOPPA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_7638_cov_3.952525"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PKH54_04275"
     CDS             complement(<1..948)
                     /locus_tag="PKH54_04275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOC99137.1"
                     /translation="MPGSCDIVAGIVNAMTLLPIYLAVVGDHRRFDARHRIVNGIAFI
                     AILLTVVVSLVDFLFVNAVDFRGALAALAGAVTLYVHSRVMGRSGFIVLAVAMACFAL
                     VFALAWFDHGGLFGTTAFLIVVMLPATLLVSPSRRRYLLFAAVALVFVALVVWEAFDQ
                     SVRIVGGLPSVRFFESMAVFIICALGAFGLFSVGMRSLRSERALAEQFAARIEALASI
                     DGLTGLLNHSAVFKAVEDEVLRTRRTGRTFSLIMVDLDDFKSVNGQFGHQYGDQILER
                     AGKAIQGCVRATDRAGRYGGEEFLVVLPETDLSGALAVAE"
     gene            1115..2650
                     /locus_tag="PKH54_04280"
     CDS             1115..2650
                     /locus_tag="PKH54_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99138.1"
                     /translation="MTNLLKLKVTGIALVALTMAIGCGGDPSPMDDNGNLEDVVVPDQ
                     GNPTDTNGQDTNGQDANIPEDTNGQDTNGQDTNGQDTNIPEDTNGQDTNGQDTNIPED
                     TNGQDTNGQDTNIPEDTNSQDTNGQDTNVVDNGTVIDVTEPPTCPANQDIANLLTQTE
                     GTVDVMICGAVVTKVTEKGWFIADDSTTRGMYIYEGTNPDWGYEVELGDYVQMHVTAY
                     GNYQGTQQVTGKENVTIQGKADYREYIVDLSEGTLPSESIESRIVRIEGVKVTAAAGK
                     NGTIDYGTATNVPVFVEHNDIPCIGLEADIVAGIISQFNAVFQVKLTEDADLANVSLA
                     NCETPPEYDMSNWDFEKQSVEEDPPEGFTKGPGNTFTCVRDADGGNNGSAGIVLTWTS
                     TDNQELVQGYKFPTAAGSTVIFALDVLDNDSAGRVRLGLSFYDADKKFITGSNSWSND
                     YSTDSADWQALSFQAVAPTGAAFAEGKLRMYDYQPPEGTWGNPTASATITIDNWEMVS
                     VAP"
     gene            2818..3630
                     /locus_tag="PKH54_04285"
     CDS             2818..3630
                     /locus_tag="PKH54_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC99139.1"
                     /translation="MKKLSNKTVLVTGGASGIGRLMSLKMAAKGARLVIWDINQANLD
                     RVVAEIRAITHDAGWGYIVDVTKPEAVYQAADKVRAECGKVDVLVNNAGIVSGKMFLD
                     VPDERIEMSLKVNTLAHFWTTKAFLPAMLESDCGHVVTIASAAGLVGVAGLGDYNASK
                     FAAVGFNESLRAEMKKRGSKVTTTVVCPFFIDTGMFDGVKTKCPLLLPILKEQYAADM
                     IVKAIESDRELLMMPRILATLPVLRILPPCMFDRVVTWLGVNSSMDEFKGRH"
     gene            3697..4323
                     /gene="pgsA"
                     /locus_tag="PKH54_04290"
     CDS             3697..4323
                     /gene="pgsA"
                     /locus_tag="PKH54_04290"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOC99140.1"
                     /translation="MKISFNNIPLKITAARLFTSPLLVLLFWMSLKGSGYGSTPEELV
                     RDLKPGLMLAAFILLLLQQVSDMVDGYIARKHKVVTNLGKLADPLADTIANFGAYLCL
                     MWVGLIPFWLLFVMYVRESSVSTLRILAAHEGVVVAARLSGKLKSLSLAIGADILFIV
                     LFAAHYYPGVPVEMVANVISWLIGIIMVVSLFDYYFAITRIRAKSRQE"
     gene            4353..6599
                     /locus_tag="PKH54_04295"
     CDS             4353..6599
                     /locus_tag="PKH54_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOC99141.1"
                     /translation="MHSFRLTSVLFAIALIFAPGTASSQPSGNSVITADDLAPAYTAQ
                     AGRKAAASAAADRFDQARDILLKGGKSVSSDHPLHARFLLAWSCYMTADWLCVESVTD
                     GLERKLPQFSDYIDLMQAASLSGLKNDRGALARLERIPATTPLRRDLLKLKAGILVSL
                     GREFDAATIIKSLVDQKDADAGLISELTEALMSSSSRDRAIAELKNIYYKSSSSGRGG
                     FRGLLRKLGVVSAPSQDETMDRAWTQLNAHANEKALKTIEPLLSETDPARRCEALEIA
                     GRACSKLRRHKEAVGHFSEVIKDCRKHRDMPPVLYTAIRSAWRSGDQARGDSWALMLA
                     TDYPDATYNDDIQVVRARTAIEQGRQHEALKIIDESLRRWPNGDMASESRWLAAWGFI
                     TAARYPDALKRLEEAIPAAGRDPSWGPRFSYWKARVMQLSGRARDARAAFSQCVIDWP
                     MSFYSVLALGRLTEGTKKAPEQALAEILADPALKPGSRGNTFLTIGDVGPEHPAARAV
                     WLSRAGLPQLAANELKMSAVRDDATDWLRALMTDKSGDFTPSHRLARDLLARNPFWPD
                     EKSAGYYRLAYPRPFEKEVRAASAEAGIDPLLVWAVMREESAFVAGIESWANAIGLMQ
                     LIMPTAKTVANQLKIPVSRERLRNPAINTRLGARYLGSLLGKFGNPVVAIPGYNAGGG
                     AIARWLKAMGDVQVDTFVESIPAWEAREYARKVFESFAAYSYLYGQQPYRIVKVDFRT
                     AKRDSGRR"
     gene            6711..>7638
                     /locus_tag="PKH54_04300"
     CDS             6711..>7638
                     /locus_tag="PKH54_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99142.1"
                     /translation="MIEQCSLFPEVLVKIFDAFNERGVENYLIGPRARDLAGGESIMD
                     AASFDLTVNASMSTIEDVFRETFGIQCNEARDDKQRLITFKLPLGDDARPGRTQITFN
                     VGPFRNYLPPLRCLRGQSLSGIILDLATREVTIQAFGYSPDGTLVDPFGGTLDYRDKL
                     IRPVFPVDTIFRESASWLLKIGRYVARYGFEAAREVRAAAERDASNILDVPRDIWLKE
                     MNKVLCGPMPARGLTFLADCRVLNFMLPEVAAMAEFSDDEGRHKDVWAHTRQVVQNAE
                     ASTVVRWAALCHDIGKVWTRQTFRDGKIHFFRH"
BASE COUNT         1613 a         2295 c         2306 g         1424 t
ORIGIN      
        1 ctcggcaacc gccagggctc cggaaaggtc ggtctcggga agaacgacaa ggaattcctc
       61 accgccgtat cgtccggcgc gatccgtggc gcgaacacac ccctgaatgg ccttgccggc
      121 acgttcaaga atctggtcgc cgtactggtg cccgaactgg ccgttgactg acttgaaatc
      181 gtcaagatcg accatgatca acgaaaatgt gcggccggtg cgacgggttc gcagaacctc
      241 atcctcgacg gccttgaaaa cggccgagtg gttaagcagg ccggtcaggc catcaatcga
      301 tgccagcgcc tcgatcctgg cggcgaactg ttcggcaagg gcccgctcgc tgcgaaggct
      361 gcgcatgccg acagaaaaca gaccgaacgc gccgagcgcg cagatgatga atacggccat
      421 cgattcgaaa aacctgacgg acggcagccc gccgacaatc ctgaccgact gatcgaaagc
      481 ctcccagacg accagggcca cgaaaaccag ggcgacggcg gcaaacaaca ggtatcgacg
      541 acgtgaaggc gacaccagca aggttgcggg gagcatgacc acgataagga atgcggtggt
      601 gccgaaaaga ccaccgtgat cgaaccaggc aagcgcgaac accagggcga aacaggccat
      661 ggcgaccgcc agcacgatga acccggaacg gcccatgacc ctggaatgaa cgtaaagggt
      721 taccgcgccg gccagggcgg ccagggcgcc gcggaaatcg acggcattga cgaaaaggaa
      781 atcgaccagg gacacgacga cggtaagaag aatcgcaatg aaggcgatgc cgttgactat
      841 gcgatgcctg gcatcgaaac gcctgtgatc cccgacgacg gccagataga ttggaagcag
      901 agtcatcgcg ttaacaatcc cggcaacgat gtcgcacgaa ccaggcaatc gatagcatcc
      961 ggtcgccgat tttatataaa tcaatgatga tttcacagga attaattcac atttcactgg
     1021 aattatcggg gagtccgctt cgtgggcctg ttcattcgat gacctttcga atatacttcg
     1081 ttcgtcttta cgcctcatac aggagacttc aaaaatgacc aacctgctca agctcaaagt
     1141 gaccggcatc gcgctggtcg cactgaccat ggcgatcggc tgcggcggcg accccagccc
     1201 gatggacgac aacggcaacc tggaagatgt cgtcgttccg gatcagggca acccgaccga
     1261 cacaaacggc caggacacca atggccagga tgcgaatatc cctgaagaca ccaacggcca
     1321 ggataccaac ggccaggata ccaacggcca ggatacgaat atccccgaag acaccaacgg
     1381 ccaggacacc aatggccagg atacgaatat ccccgaagac accaatggcc aggacacgaa
     1441 cggccaggat acgaatatcc cggaagacac caatagtcag gacacaaatg gccaggatac
     1501 gaacgtggtc gataacggca cggtaatcga cgtgaccgag ccgccgactt gtccggccaa
     1561 ccaggacatc gccaacctgc ttacccagac cgagggtacc gttgacgtaa tgatttgcgg
     1621 cgccgtcgtc accaaggtca ccgaaaaggg ctggttcatc gcggacgaca gcacgacccg
     1681 cggcatgtac atttatgaag ggaccaatcc ggactggggt tacgaagtcg aactcggcga
     1741 ctatgtccag atgcacgtca ctgcttacgg caattaccag ggaacccagc aggttaccgg
     1801 aaaggaaaac gtgaccatcc agggcaaggc cgactaccgc gaatatatcg ttgacctgtc
     1861 tgaaggcacc cttccgagtg aatcgatcga aagccgcatc gtccgcatcg aaggcgttaa
     1921 ggtcaccgcg gcggccggca agaacggaac catcgattac ggcaccgcga ccaacgtccc
     1981 cgtcttcgtc gagcacaacg acattccgtg catcggtctc gaggcggaca tcgtcgccgg
     2041 aatcatttcc cagttcaacg cagtgttcca ggtgaagctg accgaggacg ccgacctggc
     2101 gaacgtcagc ctggccaact gcgaaacccc gcccgaatac gacatgtcca actgggactt
     2161 cgaaaagcag agtgtcgagg aagatccgcc ggagggcttc accaaggggc cgggcaacac
     2221 gttcacgtgc gtccgtgacg ccgacggtgg caacaacggc agtgccggca tcgtccttac
     2281 gtggacatcg acggacaacc aggaactggt tcagggctac aagttcccga cggcagccgg
     2341 ttcaacggtg attttcgcgc ttgacgtcct tgacaatgat tcggccggca gggttcgcct
     2401 gggcctgtcg ttctatgacg ccgacaagaa gttcatcacc ggttcaaaca gctggtcgaa
     2461 cgactattcg accgactccg ccgactggca ggcgctttcg ttccaggcgg tcgcgccgac
     2521 cggggccgcg ttcgccgaag gcaagctgcg catgtacgat taccagccgc cggaaggcac
     2581 gtggggaaat ccgaccgctt cggccacgat taccatcgac aactgggaaa tggtttccgt
     2641 cgccccgtaa ttgacctggt tcagtgatca ttgcaattca tgcggcgggc gaattttcat
     2701 tcgcccgtac ggattctggt gcggtgcgtg agtccggttg cggcgggcga attttcattc
     2761 gcccgtacgg attatggtgg ggtgcgtgcg tccggttgaa cgcggcaggt gtttattatg
     2821 aaaaaacttt cgaacaagac tgttctggtc accggcggcg cttcggggat tggcaggctg
     2881 atgtcgctga aaatggcggc gaagggcgcc aggctggtaa tctgggacat caaccaggcc
     2941 aaccttgacc gcgtcgtcgc cgaaatccgt gccatcacgc atgacgccgg ctggggttac
     3001 atcgtcgatg taacgaaacc cgaggccgtc tatcaggccg cggacaaggt tcgcgcggaa
     3061 tgcgggaagg tggatgtcct ggtcaacaac gcgggcatcg tcagcggaaa gatgttcctg
     3121 gacgttcccg acgaacgaat cgagatgtcg ctgaaggtca acacccttgc gcacttctgg
     3181 acaaccaagg cattccttcc ggccatgctc gaatcggact gcggtcacgt cgtgacgatc
     3241 gcttcggcgg ccggacttgt cggcgttgcc ggacttggcg actacaacgc cagcaagttc
     3301 gcggcggtgg gattcaacga atcgcttcgc gccgaaatga agaagcgcgg ttcgaaagtc
     3361 accacgacgg tcgtctgccc gttcttcatc gacaccggca tgttcgatgg cgtcaagacg
     3421 aaatgccctc ttctgcttcc aatcctgaag gagcagtacg cggccgacat gatcgtcaag
     3481 gcgatcgagt ccgaccggga actgctgatg atgccgcgta tccttgcgac cctgccggtt
     3541 ttgcggattc tgccgccgtg catgttcgac cgcgtcgtca cctggctcgg tgtcaattcc
     3601 tcgatggacg aattcaaggg acgccactga cccgccccgc atcaattttt ccggattttg
     3661 ttggtcaagc gggctccaat cggatacgat agccgcatga aaatcagctt caacaacatt
     3721 ccactcaaga tcaccgcggc aaggttgttc accagcccgc tgctggtact gcttttctgg
     3781 atgtcgctga agggttccgg atacggcagc accccggaag aactggtgcg cgatttgaag
     3841 cccggcctga tgctggccgc tttcatcctg ctgcttctgc aacaggtcag cgacatggtt
     3901 gacgggtaca tcgcgcgcaa gcacaaggtc gtcacgaacc ttggcaagct ggcggatccc
     3961 ctggccgata ccatcgccaa cttcggcgcg tatctttgcc tgatgtgggt cggcctgatc
     4021 ccgttctggc ttcttttcgt gatgtacgtg cgcgaatcgt cggtcagcac ccttcgcatc
     4081 cttgccgccc atgaaggcgt cgtcgtcgcc gccaggcttt cgggcaagct gaagtccctg
     4141 tcgcttgcaa tcggtgccga catccttttc atcgtgctgt tcgcggccca ctactatccg
     4201 ggcgttccgg tcgaaatggt cgcgaacgtg atctcgtggc ttatcggcat catcatggtg
     4261 gtgtcgcttt tcgattatta cttcgccatc accaggatcc gggcgaaatc ccgccaggaa
     4321 tagattcccg gaccgaaaga ctggttcttc cgatgcattc gttcaggctg acgtcagttt
     4381 tgttcgccat cgcgctgatt ttcgcgccgg gaacggcttc atcccaacca tccggcaaca
     4441 gtgtcatcac ggcggacgat ctcgccccgg catacaccgc acaggctggg cgcaaggcgg
     4501 cggccagcgc ggcggcggat cggttcgacc aggccaggga catcctgctg aagggcggaa
     4561 agtccgtttc gtcggaccac ccgcttcacg cccgcttcct gctggcctgg tcgtgttaca
     4621 tgaccgccga ctggctgtgc gtcgaatcgg taacggacgg tttggaaagg aaactgcctc
     4681 agttttcgga ctacatcgac ctgatgcagg cggcttcact ttctggattg aagaacgacc
     4741 gcggcgccct ggcacgactt gagcgcatcc cggccaccac cccgcttcga cgggatctgc
     4801 tgaaactgaa ggcgggaatc cttgtctcgc ttggacgtga attcgacgcc gcgaccatca
     4861 tcaaatcgct ggttgaccag aaggacgccg atgcgggcct gatttccgag ttgaccgaag
     4921 cgctgatgtc gtcatcgtcg cgcgatcgcg cgatcgccga actgaagaac atttattaca
     4981 agtcgtcttc atcgggccgt ggtggatttc gcggtctttt gaggaaactt ggcgtcgtgt
     5041 cggcgccgtc ccaggacgaa acgatggatc gcgcctggac ccagttaaac gcgcacgcaa
     5101 acgaaaaggc actgaagacg atcgagccgc ttctgtcgga aacggatccg gcaaggcgct
     5161 gcgaggcgct tgagatcgcc ggacgggcgt gttcgaagct gcggcgtcac aaggaagccg
     5221 tcgggcactt ttcggaagtc atcaaggact gccgcaagca ccgcgacatg ccgccggttc
     5281 tttacaccgc gattcgaagc gcctggcgat cgggcgacca ggcacgcggc gactcgtggg
     5341 cactgatgct cgcgaccgac tacccggacg ccacatacaa cgacgacatc caggtcgtgc
     5401 gcgcccgcac cgcaatcgag cagggccgcc agcacgaggc cctgaaaatc atcgacgagt
     5461 cgctgcggcg ttggccgaac ggcgacatgg cttccgaatc ccgctggctt gcggcctggg
     5521 gtttcatcac ggcagccagg taccccgacg cgctgaagcg acttgaggag gccatcccgg
     5581 cggctgggcg ggatccttcc tgggggccac gtttcagtta ctggaaggcg cgtgtcatgc
     5641 agctttccgg acgcgccagg gacgcccgcg cagccttcag ccagtgcgta atcgactggc
     5701 cgatgtcgtt ttattcggtg cttgcgcttg gccgcctgac cgagggcacg aagaaggccc
     5761 cggagcaggc cctggcggaa attcttgcgg atcctgcctt gaagcctggt tcgcgcggca
     5821 acacgttcct gacgatcggg gatgtcggcc cggaacaccc ggccgcgcgc gcggtctggc
     5881 tttccagggc gggtcttccg cagcttgccg cgaacgaact gaagatgtcg gcggttcgcg
     5941 acgacgcgac cgactggctg cgtgcgctga tgacggacaa gtccggggat ttcacgccat
     6001 cgcaccggct ggccagggat cttctggccc gaaacccgtt ctggccggat gagaagtcgg
     6061 cgggttacta caggcttgcc tatccgcgcc cattcgaaaa ggaagtccgc gcggcttcag
     6121 cggaagcggg tatcgatccg ctgctggtct gggccgtaat gcgcgaggaa agcgcattcg
     6181 tggccggaat cgaatcctgg gcgaacgcaa tcgggctgat gcagcttatc atgccgactg
     6241 cgaagaccgt cgcgaaccag ttgaagatcc ccgtttcgcg ggaacgcctg cggaatccgg
     6301 ccatcaatac ccggcttggc gccaggtatc ttggcagcct gcttggcaag ttcggaaacc
     6361 cggtcgtcgc gattcccgga tacaacgcgg gtggcggcgc cattgccagg tggctgaagg
     6421 cgatgggcga cgtccaggtc gatacgtttg tcgaatccat tcccgcctgg gaggcaaggg
     6481 aatacgcgcg caaggtgttc gaatcgtttg ccgcttacag ttacctgtac ggccagcagc
     6541 cgtacaggat cgtcaaggtc gatttcagga cggcaaagcg cgattcgggc cgtcgttgat
     6601 accggtcgcg ggttgacgcg aattgtgcgg ttcgcgtatt cttcaccgcc gcttccggcc
     6661 gcaaggccgc cgggcgccgg aaccacgacc atacagggtg atttctttcg atgattgagc
     6721 aatgcagtct ttttccggaa gtgctggtca agatcttcga cgcgttcaac gaacgcgggg
     6781 tcgaaaacta tctgatcggg ccgcgcgcgc gtgacctggc cggcggcgag tccatcatgg
     6841 atgccgcgtc cttcgacctg acggtcaacg cttcaatgtc aacgatcgag gacgttttcc
     6901 gcgagacgtt cggcattcag tgcaacgagg cgcgcgacga caagcagaga ctgattactt
     6961 ttaagctgcc actcggggac gacgcgcgcc ccgggcggac acagattacg ttcaacgtcg
     7021 ggccgttccg caattacctg ccgccgctgc gctgcctgcg cggccagagc ctgtccggga
     7081 ttattcttga tcttgcgacc cgcgaagtta ccatccaggc gttcgggtac tccccggacg
     7141 gcactctggt cgatccgttc ggcggcacgc tggattaccg cgacaagctg atccgcccgg
     7201 tcttcccggt cgataccatc ttccgcgaaa gcgcaagctg gcttctgaag attggccggt
     7261 acgtggcccg ttacggattc gaggccgcca gggaagtccg cgcggccgcc gagcgcgacg
     7321 cttccaatat cctggacgtg ccgcgcgaca tctggctgaa ggaaatgaac aaggtcctgt
     7381 gcggaccgat gccggctcgt ggcctgacgt tccttgcgga ttgccgcgtg ctgaacttca
     7441 tgctgccgga agtcgccgcc atggcggaat tttcggacga cgaaggccgc cacaaggacg
     7501 tgtgggcgca cacccgacag gtcgtacaga acgcggaagc aagcaccgtt gtcaggtggg
     7561 ccgcgctttg ccacgacatc ggcaaggtct ggacgcgcca gaccttccgc gacggcaaga
     7621 ttcacttctt ccgccacg
//
LOCUS       DAOPPA010000096         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10708_length_7634_cov_3.746273, whole genome shotgun sequence.
ACCESSION   DAOPPA010000096 DAOPPA010000000
VERSION     DAOPPA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10708_length_7634_cov_3.746273"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKH54_04305"
     CDS             <1..333
                     /locus_tag="PKH54_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HOC99143.1"
                     /translation="FDRDRLSRLGVSVDSHGKMPDVILFLEDRNQLVLVESVTSHGPV
                     DAKRHHELSRLFGKSSAGLIFVTAFPDRSLMGRYLTDIAWESEVWVANAPSHLIHFNG
                     DRFLGPHP"
     gene            372..1124
                     /locus_tag="PKH54_04310"
     CDS             372..1124
                     /locus_tag="PKH54_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOC99144.1"
                     /translation="MIGPIRRFRRWKISRRPFPQEWRGYLSEHFACYRMMPPEWRNRY
                     EHMIRIFIAERHWIPAAGMVIDDRVKVIIAGAAVRLVLRLGLEYYDRMREIVVYPYVY
                     KHKDERGMMIFGEAHPWGTVVLSWPAVLHGLANPCDGENTALHEFAHILDRDDGAFNG
                     TPFLHSREDYGPWAEVMARHFELLKLGGPEELAVLRMYGATNPAEFFSVATEAFFEIP
                     SVMQGQMPELYEVMKKFYGGDPFTYGLCPRRL"
     gene            complement(1340..2815)
                     /locus_tag="PKH54_04315"
     CDS             complement(1340..2815)
                     /locus_tag="PKH54_04315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC99145.1"
                     /translation="MTFDDLNLHPTLLKAVHDQGYDVPTPIQEQAIPLVLTGGDLLGC
                     AQTGTGKTAAFALPILQNLSQNRPPLATRQGGVRQIRCLVLAPTRELALQIHESFCAY
                     GRYTGMRSTYIFGGVSQNPQVKALQQGVDILVATPGRLLDLIGQNFINLRYVEVFVLD
                     EADRMLDMGFINDIRKVVAQLPKMRQTLLFSATLQPEIRNLAAGMLRDPATVSVAPPA
                     STVDRIQQSIHFVEKAEKINLLLRLLENSDVERALVFTRTKHGADKVVRKLTQAGIMA
                     AAIHGNKSQNNRQAALDAFKEGRSRVLVATDIASRGLDIDEVSHVFNLDLPMEPEAYV
                     HRIGRTARAGHEGIAVAFCSSDEKSLLTQIERMIGKRIPAAGEGNRSQAERQAPRFAE
                     RVEGGDGRRMRPSERPQVAAAGQGPREGADQQQGTRMMGEKFGGVRVTTETRTPNLHP
                     GTHQALLRDQDPNAPERRSSGRTGNARPLPQRYQPRGRGQR"
     gene            complement(3126..4760)
                     /locus_tag="PKH54_04320"
     CDS             complement(3126..4760)
                     /locus_tag="PKH54_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99146.1"
                     /translation="MKMMKNVVSGAVVPLLMLWGLGCGQGGVASDTGDIGQDAIQADT
                     TADSSTPGDAVLDVRADALPDGVVPDSSKVDTGTDVTANDVVDDDGTPVDDIPDTSEC
                     PEPLALACGDSFDHDTGVQGRANIWGGYSCTARALSGRETVYLLKTDEHVNVTVTLTN
                     LSVDLDVARLSKCDPFSCRNIASTPLDIQDDEQIQFESGIGHQQYIVVDGYAGATGTY
                     TIGVTCEPVPEPECPPHLSRLVECPFIWGEPGNGAVACPSTAECLGVPCAQDGFCALY
                     AEEGGGDKCVMGNCVYCWQDSQCPQGDVCRAGRCVGKTPAECPEVPDCAAAGCLEMML
                     SDTNCPTCVCDTEFFNQCEIDDDCMPISHHLFRRCVYGRCAECRFDDDCDSGECLPPG
                     ICYSMTKHPSAIFGTWLIGWYGGLDHFSYFRFEPDGTLRRAGYVPESTFADDILTGGC
                     AAEAGQSLPGIVGTWEPEVTESGFLVVRLAFVSPCQAGTKVSERFAITMGTDGDSMTL
                     KSVENPDAGDLTGLRINPLLCSADFTTCPSPVYPLL"
     gene            5010..7304
                     /locus_tag="PKH54_04325"
     CDS             5010..7304
                     /locus_tag="PKH54_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99147.1"
                     /translation="MRERFPVLSMVVLAALINGCGTSDGESEGNPESPYKVDRPECGG
                     TWNGGQIQGAEGLVIDVPSGQVSTCSNLDSQPSVSVQTIIPDDFPITMPEENVVTADG
                     DVRFIDSGDGFNLSPGSFVTLTVPYSPFRFPVGAIEDSTNTFVRIYEPERDVASDVHG
                     VVDVDNHTVTVQIIGLPPSAIVSVIHRQARVAVAMGSVPVAAPRSWKATDFPPWPGRD
                     WCVIHDYDSAELREAVRKSGNMESDPTPQMIAQTAIDKVAAAARVAQATYEGLGLRAP
                     WLYVSSDPEGACGDVFGTTPRYEIQLIDGRGSHYKSQDPDEATGPGDRRYGRIYIRSS
                     RIGDDATSALGTVKASVAHEMLHAVQMGYEILVDSVDGLNEGTATVAGLMIDTGTVGV
                     RTANDAEVFRLPWFLMADAAGVMYSNQDFFAFVARRYSGGSIAMLPVVFQAMRDGINS
                     LTSGATTESGADAMRIVPGIRAVHQALDAAIKSAMPGIDLRQMYADFLIQRALEHGPE
                     SVFGRPGETTTGLADELFGVTVGQWGLIREVSATMAECGLSKTVVKLDPVYPFSARAV
                     RVTATDVPNPPRKATLSVSVSGDAGQDVIAFGMDGDLVQTIEGPMTFEDFGSWEGHAF
                     EMVVAMAGWSMEKTGITVELGLACDEPPPQGEDPMKCGSSGIPACNDVPAEYYWRRGC
                     EEYTGSHWADDWQSLKTMCGFTNGSEWCDSGCSRSNILGTCSESMGENAEVVFYFYTT
                     VDYAAQGFDPADNCSKEGGVWTGY"
     gene            complement(7341..>7634)
                     /locus_tag="PKH54_04330"
     CDS             complement(7341..>7634)
                     /locus_tag="PKH54_04330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOC99148.1"
                     /translation="RKIDTIGRWGGDEFVCVVPHTDGEELDVLAERLRVNVRRLQIDL
                     DENRRIRPSLSIGTATIRPGDTTDSLIARADLAMYENKRLVKAACYVRVNTDA"
BASE COUNT         1413 a         2258 c         2372 g         1591 t
ORIGIN      
        1 tttgatcgtg acagattgtc aaggcttgga gtcagcgttg acagccatgg caaaatgccc
       61 gacgtcattt tgtttcttga ggatcggaac cagttagttc ttgttgagtc cgtcacaagt
      121 catggtccag ttgatgccaa gcggcaccat gaactttcca gattgttcgg taaatcatcg
      181 gccgggttga tttttgtgac ggcatttccc gatcgatcat tgatggggcg ctatcttacc
      241 gacatcgcat gggaatctga ggtctgggtg gccaacgctc cttcccacct cattcatttc
      301 aatggcgacc gcttccttgg gcctcatccg taggttgggg cgatatttac gggttctgct
      361 acaatgccgg catgattggg ccaatcagac gattcaggcg atggaagata agcaggcggc
      421 cgttcccgca ggaatggcgg ggttacctgt ccgaacattt cgcctgttac cggatgatgc
      481 ctcccgagtg gcgcaaccga tacgagcaca tgattcgcat attcatcgcc gaacgccact
      541 ggattccggc ggccggcatg gtgatcgacg atcgtgtgaa ggtcatcatc gcgggcgccg
      601 cggttcggct ggtgcttcgg ctgggcctgg aatattacga ccggatgcgc gaaatcgtcg
      661 tatatccgta tgtctataag cataaggacg agcgcggaat gatgattttc ggcgaggcgc
      721 atccgtgggg aaccgtggtg ctgtcgtggc ccgccgtgct gcatggtctt gccaacccgt
      781 gtgacggcga aaacaccgcg ctgcacgaat tcgcccacat cctggatcgc gacgacggcg
      841 cgttcaacgg cacgccattc cttcacagcc gcgaagacta cgggccatgg gccgaagtca
      901 tggcccggca tttcgagctg ctcaaactgg gtggcccgga agaactggcc gtgctgcgca
      961 tgtacggcgc gaccaacccg gccgagttct tttccgtggc gaccgaagcc ttcttcgaaa
     1021 tcccgtcggt gatgcaggga cagatgccgg aactgtacga agtcatgaag aaattctatg
     1081 gcggcgaccc gttcacgtac ggcctgtgcc cgcgcagact ataatttcag gggacgaaag
     1141 tcccagaacc gatctgcccc gacaccgata acgtggtcgt ggacctgctg ccctgaatac
     1201 tggacgcatc tgccagtcgg tcattttttg aacgcatttt tcgggtcttc acgtatcgcg
     1261 acgagcggca acctttcctg gcggtcaatc cggtttccac gtcagtgaag atctgacgac
     1321 atttcctggg ggtcgttggt cagcgctggc cgcggccacg gggctggtaa cgctggggca
     1381 ggggacgggc gttgccggtg cggccggatg aacggcgctc gggcgcgttc gggtcctggt
     1441 cgcgcagcag ggcctggtgg gttccggggt gaaggttcgg tgtccttgtt tcggtggtta
     1501 cgcgcacccc gccaaacttt tcgcccatca tccgcgttcc ctgttgctgg tcggcaccct
     1561 cgcgcggacc ttgcccggcc gccgccacct gcgggcgctc ggacggccgc atccggcggc
     1621 cgtcgccccc ttcgacacgt tccgcgaaac gcggcgcctg cctttccgcc tggctcctgt
     1681 tgccctcgcc ggcggcaggg atgcgcttgc cgatcatcct ttcgatctgg gtcagcagcg
     1741 acttttcatc cgaagagcag aacgccaccg cgattccctc gtgtccggca cgtgcggtgc
     1801 gaccgatccg gtgcacgtac gcttccggct ccatcggcag atccaggttg aacacgtgcg
     1861 acacttcgtc gatatccaga ccgcgtgatg cgatatcagt cgccacaaga acccggctgc
     1921 ggccttcctt gaaggcgtca agcgctgcct ggcggttgtt ctgcgacttg ttgccatgaa
     1981 ttgccgccgc catgatgccc gcctgggtca gcttgcggac aaccttgtcg gcgccgtgct
     2041 tcgtgcgcgt gaacaccagc gcccgctcga catcggaatt ttcaagcagc cgcagcagca
     2101 ggttaatctt ttccgccttt tccacgaagt ggatggactg ctggatgcga tcgaccgtcg
     2161 aagcgggcgg ggccacgctt accgttgccg ggtcgcgcag catgccggcc gccaggttgc
     2221 ggatttcggg ctgcagcgtc gccgaaaaca gcagcgtctg gcgcattttc ggcaactggg
     2281 ccaccacctt tcggatatcg ttgatgaatc ccatgtccag catgcggtcg gcctcgtcca
     2341 ggacgaacac ctcgacgtat cgcaggttga tgaagttctg gccgatcagg tccagcagcc
     2401 gtcccggcgt ggcaaccagg atatcgacac cctgctgtag tgccttgacc tgcgggttct
     2461 ggctgacccc cccgaagatg taggtcgagc gcatgccggt atatcttccg tatgcgcaga
     2521 agctttcgtg aatttgaagt gccagttccc gtgtcggggc cagaaccagg caacggatct
     2581 ggcgcacgcc gccctgtctg gttgccagcg gcggtcgatt ctgggaaaga ttctgaagaa
     2641 ttggcagcgc gaatgccgcc gtcttgccgg tgccagtctg ggcgcagccc agcaggtccc
     2701 cgcccgtcag gaccagtgga atggcctgtt cctggatcgg ggtcggaacg tcatagccct
     2761 ggtcgtgaac tgccttcaga agggtggggt gtagattgag gtcgtcgaaa gtcatggata
     2821 cctttacatt tcctgatgtc gttcaggtgt gacggcaaac ggggcctgga tcgagcaaaa
     2881 tcggggcttc caatcgatcg gaaacccgga ggcgtaaatc aagtgttacg cgaactcgcc
     2941 gaggaatcgc ctgcggttgt caggactgta agtcaaaaaa tcaagaatgt caagggcttg
     3001 gatgtcgggt gcggtcccgc gcccctctta aggcgatgaa attgcagact attgttgtcg
     3061 cagcgggact gcatccggcc atgtaaggaa cgtctgccgt gaagccgaag acgcttccgg
     3121 gaaattcaca aaagtgggta aaccggtgac ggacaggtcg tgaagtccgc cgagcacaga
     3181 agcgggttga tccgaagccc ggtcaggtcg cccgcgtcgg gattttccac gcttttcagc
     3241 gtcatgctgt cgccgtcagt acccatcgtg attgcgaacc gttccgacac cttcgtcccg
     3301 gcctggcaag ggctgacgaa tgcaaggcgc acgaccagga agccggactc ggtcacttca
     3361 ggttcccatg tgcccacgat gcccggcagc gactggcccg cttcggcagc gcatcccccg
     3421 gtcaggatgt cgtccgcgaa tgtgctttcc ggaacataac cggcccgccg caaggttccg
     3481 tccggttcaa acctgaagta gctgaagtgg tccaggccgc cgtaccaccc gatcagccag
     3541 gtgccaaaaa tggccgacgg gtgctttgtc atcgagtagc agatgcccgg gggcaggcat
     3601 tccccgctgt cgcagtcgtc gtcgaaccgg cattcggcgc agcgcccata gacgcagcgc
     3661 ctgaacaggt ggtgtgatat cggcatgcag tcatcgtcga tctcgcactg gttgaagaat
     3721 tcggtgtcac agacgcaagt cgggcagttg gtgtctgaca gcatcatttc caggcagcct
     3781 gcggccgcgc agtccgggac ttcggggcat tcggccggtg ttttcccgac gcagcgcccg
     3841 gcccggcaga catcgccctg cgggcactgg ctgtcctgcc agcaatacac gcaattgccc
     3901 atgacgcact tgtcgccgcc gccttcctcg gcgtacagcg cgcagaagcc gtcctgggcg
     3961 caaggcaccc caagacattc ggccgtggaa gggcaggcga ccgccccgtt gcccggctcg
     4021 ccccagatga acgggcactc gaccagcctg gaaaggtgcg gcgggcattc aggctcggga
     4081 accggttcgc atgtgacgcc aatcgtgtaa gttcccgtgg cgccggcata tccatcgacg
     4141 acgatgtact gctgatggcc aatcccggat tcgaactgga tctgctcgtc gtcctggatg
     4201 tcaagcggtg tcgacgcgat gttgcggcaa ctgaacgggt cgcatttcga aaggcgcgct
     4261 acgtccagat cgactgaaag attcgtcagg gtcacggtga cgttgacgtg ttcgtcggtc
     4321 ttaagcaggt aaaccgtctc acgtcccgaa agtgccctgg cggtgcagga atagccgccc
     4381 caaatgttgg cccgtccctg gacgcccgtg tcgtggtcaa acgaatcgcc gcatgccagt
     4441 gcaagcggtt ccggacattc gctggtgtcg ggaatgtcgt caaccggggt tccatcgtcg
     4501 tcaacgacgt cattggctgt tacatccgtc ccggtatcga ccttcgatga atccggcacg
     4561 acaccgtcgg gcagcgcgtc ggcacgcacg tccagaaccg cgtcgcccgg cgtgctcgaa
     4621 tccgcggtgg tgtcggcctg aatcgcgtcc tggccgatgt cgcccgtgtc cgaagcaacg
     4681 cccccctggc cgcacccaag accccacagc atcagcaacg gcacgacggc gcccgaaacc
     4741 acatttttca tcattttcat cgtgttacta cctggttctc ctgactgccg tcaggttact
     4801 gcttttgggc ccgcataaca accggccggg cgccgacagg cagtcttgtc gcggatggaa
     4861 tgtaaaggtt gcacgccccc acttgacgcc ccgggcgggc caagagtagc ttgtggcaat
     4921 cggggcatcg ggacgttggg gcatgtttca tagggacgtc cggtatcggg aggtcaaaat
     4981 ggcgcatttc ggacggtggg ggttatgtga tgcgtgaaag atttccagtt ctgtcgatgg
     5041 tcgttctggc agccttgatt aacggttgcg gcacctctga tggcgagtcc gaaggcaatc
     5101 cggaatcccc gtacaaggtc gatcgcccgg aatgcggcgg aacctggaac ggtggacaaa
     5161 tccagggcgc ggaaggcctg gtcatcgacg tcccgtccgg acaggtttcc acatgttcaa
     5221 acctggattc ccagccgtca gtgtccgtcc agaccattat tccggacgac tttccgatca
     5281 ccatgccgga agaaaacgtg gtcacggccg atggcgacgt caggttcatc gattcagggg
     5341 acggattcaa tctttcgcca ggctcgtttg tcacattgac ggtaccctac agcccgttcc
     5401 gtttcccggt cggcgcgatc gaggattcga caaacacgtt cgtcaggatt tatgaaccgg
     5461 aacgggatgt tgcgtcagac gttcatggcg tggtcgatgt cgataatcat accgtgactg
     5521 tccagatcat cgggcttcca cccagcgcca tcgtttcggt aattcaccgg caggcccgcg
     5581 tggccgtcgc gatgggcagc gtccctgtcg cggccccaag atcctggaaa gcgaccgact
     5641 ttccgccgtg gcccggtcgc gactggtgcg tgattcatga ctacgactca gccgagcttc
     5701 gcgaggctgt caggaagtcg ggcaacatgg aatcggatcc gactccgcag atgattgccc
     5761 agaccgcgat tgacaaggtc gccgctgccg cccgggtcgc ccaggccacc tacgagggcc
     5821 tgggattaag ggccccctgg ctttacgtgt cttcggatcc ggaaggtgca tgcggcgatg
     5881 tgttcgggac gaccccacgg tatgagatcc agttgattga cggtcgtggc agccattaca
     5941 aatcgcagga tcccgacgaa gccactggtc cgggtgaccg ccggtacggc cgcatctata
     6001 taagatccag ccgcatcggg gatgacgcga catcggctct tggcaccgtc aaggcgtccg
     6061 ttgcccacga gatgctgcac gcggttcaga tgggatacga aatcctggtc gattccgtcg
     6121 atggccttaa cgaaggcacc gcaacggtgg ccggattgat gatcgacacc gggacggtcg
     6181 gggtcaggac cgccaacgat gccgaggttt tcaggctgcc gtggttcctg atggccgatg
     6241 cggcaggcgt catgtattca aaccaggatt tcttcgcctt tgtggcccgc cggtattccg
     6301 gtggctcaat tgccatgctt ccagtcgttt ttcaggcgat gcgggacggc atcaattcat
     6361 tgacgtccgg ggcgacgacg gaatcgggtg ccgacgcgat gcgcattgtt ccgggaatcc
     6421 gcgccgtgca ccaggccctg gacgccgcca tcaagtccgc gatgccgggg atcgatttgc
     6481 gacagatgta tgcggatttc ctgatccagc gcgcccttga acatggtccc gaatccgttt
     6541 tcggtcgccc cggcgaaacg accaccggcc tggccgatga gcttttcggg gtgacggtgg
     6601 ggcagtgggg cttgattcgt gaagtgtccg ccacgatggc ggaatgcggg ttgtcgaaga
     6661 ctgtggtgaa gctggatccg gtgtatccct tttctgcccg cgccgtaagg gtcacggcga
     6721 cggacgtgcc gaatccgccc cgaaaggcga ccttgagcgt ctcggtttcg ggcgatgccg
     6781 gtcaggacgt catcgcattc gggatggatg gcgacctggt tcagaccatt gaaggtccga
     6841 tgacgttcga ggattttgga tcctgggaag gtcacgcctt cgaaatggtc gtcgcgatgg
     6901 cgggctggtc aatggaaaag accggaatca cggttgaact gggacttgcc tgcgacgaac
     6961 ctccgccgca gggtgaagac ccgatgaagt gcggttcgtc cggcattccg gcatgtaacg
     7021 acgttcctgc cgaatactac tggcggcgcg ggtgcgagga gtacaccggc agccactggg
     7081 ccgatgactg gcagtcgctc aagacgatgt gcggttttac aaatggatcg gagtggtgcg
     7141 attcgggctg cagccgttca aatatccttg gaacgtgcag tgagtcgatg ggagaaaatg
     7201 ccgaggtggt cttttacttc tacacaaccg ttgactacgc cgcccagggt ttcgatccgg
     7261 ccgacaattg ttcgaaggaa ggtggggtct ggaccggata ctagccgcgc cgtcctgccc
     7321 ggcggcctcg cggatccgaa tcatgcatcg gtgttcacgc gcacatagca ggccgccttg
     7381 accaggcgct tgttttcgta catcgccaga tccgcgcggg caatcaggct gtcggtcgtg
     7441 tcgccgggcc ttattgtcgc cgtcccgatt gacagtgacg gtcgaatcct tctgttttca
     7501 tccaggtcaa tctgcagccg ccgcacgttg acgcgcaggc gttcggcaag cacgtcgagt
     7561 tcctcgccgt cggtatgggg aaccacgcag acgaattcat ccccgcccca gcgcccgatc
     7621 gtgtcgatct ttcg
//
LOCUS       DAOPPA010000097         7581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10789_length_7581_cov_3.698512, whole genome shotgun sequence.
ACCESSION   DAOPPA010000097 DAOPPA010000000
VERSION     DAOPPA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_7581_cov_3.698512"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..377)
                     /locus_tag="PKH54_04335"
     CDS             complement(18..377)
                     /locus_tag="PKH54_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC99149.1"
                     /translation="MKISITVTDEAVLRELGTRIARHRIDRGLSQHALASGAGIAKRT
                     LERLESGDPVQLTTLVRVLRVLGLLDMLDLVVPEIQIQPVRLLQEGTRQRRRAPRKAS
                     GGGSTGTPSSWKWGDEK"
     gene            complement(471..1130)
                     /gene="elbB"
                     /locus_tag="PKH54_04340"
     CDS             complement(471..1130)
                     /gene="elbB"
                     /locus_tag="PKH54_04340"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOC99150.1"
                     /translation="MKKVAVIMAGCGFKDGGEIHENTLTLLALNLAGAQYQCAAPSIP
                     QFQVVDHVTAQRVAETRNVMVEAARIARGNIVDVRELRVADYDAVVVPGGFGAANNLS
                     SFAREGAAMKVDPGVGEFLIGMHKAGKPIGAICIAPPLAAWTLKQCGVTGADITIGND
                     PGTAAKIRELGQNHVDCPATDCVVDRRHKVVTTPAYMTARSIGELWAGIQRTIEELLK
                     L"
     gene            1313..2551
                     /locus_tag="PKH54_04345"
     CDS             1313..2551
                     /locus_tag="PKH54_04345"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOC99151.1"
                     /translation="MSKITSEFIAEIPKTDLHVHLDGSLRIPTLIDLAQSAGVALPSY
                     TDEGLRELVFKDKYANLVEYLQGFGLTTSVMTNAEQVERVAYELAEDNFNEGVRYFEV
                     RFAPQLHVTADFNLDQVLLAVNRGLAKATSDFNARNALIPADERGPDGHYGIIVCAMR
                     NAMPVLSPYFQDVFRVHAYSTDRAVGSMTSLEMARAAVDIRDRLGIPITGFDLAGAEA
                     GYPAVEHRDAFDYCHKNFLKKTVHAGEAYGPESIFQAITELHADRLGHGYYLLSPDKI
                     VSRAIADPQDYVRRLSEYIADRRITIEVCLTSNMQTNPSITDLKHHAFGKMLKARLST
                     TLCTDNRLVSNTTVTREIQLAVDTFGIGPSELKDIIVYGFKRSFYHGSYNEKRQYVRS
                     VIDYYEALERKHGIRHAPNT"
     gene            2560..4587
                     /gene="uvrB"
                     /locus_tag="PKH54_04350"
     CDS             2560..4587
                     /gene="uvrB"
                     /locus_tag="PKH54_04350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOC99152.1"
                     /translation="MFKLVSDYKPAGDQPQAISRLVNLLQDGGRDQVLLGVTGSGKTF
                     AMASVIAEIGRPALIMAPNKTLAAQLFGEFKAFFPENAVEYFVSYYDYYQPEAYVPAS
                     DTYIEKDSKINEEIDRMRHSATRSLFERDDVIIVASVSCIFGLGSAEAYYGLVEFLSV
                     GQHCDRDALLRRLVELQYDRNDIAFERGTFRVRGDVVEIFQPFEEQTSLRIVLDCDEI
                     ESITEIDPVSGHRLRNLPKVAIYPNSHYVTTPERMRRAIVGIQDELQERLVEFKESGR
                     VLEAARIEQRTLSDIEALEQFGRVAGIENYSRHLTGRVAGEAPPTLLDYFPKDFLCFL
                     DESHMTVPQLHGMYRGDRARKDVLVNYGFRLPSAVDNRPLRFDEFDERRGRTIYVSAT
                     PGDFEMKRSGAVGPDGSPDPNSESGRIARMIVRPTGLLDPVIDVRPATNQVDDLLDEI
                     RAVVDRGQRILVTTLTKKMAEHLTEYFTEAGLKVNYLHSDVHTLERIQIIKDLRAGVF
                     DVLVGINLLREGLDLPEVALVAILDADKEGFLRSERSLIQTFGRAARNTDALVIMYAD
                     TVTRSMQAAMDITTARRQRQIEFNERHGITPTTVARRLFFVEEAEHAAVVAGVKDPNA
                     LSDLVMDPKLLGKTLNQLKKQMLEASAKLEFERAAEIRDRIRAIRNVMGNL"
     gene            4617..4961
                     /locus_tag="PKH54_04355"
     CDS             4617..4961
                     /locus_tag="PKH54_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC99153.1"
                     /translation="MLVRKKGTSEHIPVMMTGASKVSKQITVGRADGTPAYSMRIFTV
                     EPGGHTPFHSHPFDHVNFVVSGSGVLVSAGGQTPVEAGDSALVLPDEMHQYRNTGSEP
                     FVFICFVDKAYE"
     gene            complement(5034..5558)
                     /locus_tag="PKH54_04360"
     CDS             complement(5034..5558)
                     /locus_tag="PKH54_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99154.1"
                     /translation="MSDNPSYKLGQFIDIYCRYCRLNLDAVVASMNPDGTLAKVQCRT
                     CMHFQDFKPPADLQEKHRKLVEKAMKIAEKHTRGPAAAEAKAKKDAAAKASAADLRPE
                     AVMRRLWDEATANVSPMKMKVYDKHRSYHKDDVITHKVHGLGVVVRDDVEYSMTVLFR
                     DQVVELDCDQPRED"
     gene            complement(5593..5991)
                     /locus_tag="PKH54_04365"
     CDS             complement(5593..5991)
                     /locus_tag="PKH54_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC99155.1"
                     /translation="MNSFEVRTVRPIQTIDITDRVAEVLTGSGIDQGAVLVWCPHTTA
                     GIFCNEGADPDVVRDVESTLERIYPFCTEYLHAEGNSHAHVRSIMMGNSLTIPVVGGK
                     MSLGTWQHVFFAEFDGPRTRQVWVQPLRGL"
     gene            6126..6410
                     /locus_tag="PKH54_04370"
     CDS             6126..6410
                     /locus_tag="PKH54_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99156.1"
                     /translation="MADDRATSGVTEAETIDLDNIHTDHTPSDLFKVGFVYGLGGGGV
                     TAIIGVRFLVFGNGAQEHAAGIMFFVVSGLMLFAGVWCHVKRFRMLRNKA"
     gene            complement(6411..>7581)
                     /locus_tag="PKH54_04375"
     CDS             complement(6411..>7581)
                     /locus_tag="PKH54_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC99157.1"
                     /translation="LHGALFKALSPEPGDRFPDMDGFADALHDAVEAMSTAISAEQSD
                     GPTVLEVRGARGDESVPAPVSTADPVGAAGSDASIVDALDLSPVAAKLSEKKEDRPQL
                     RARRRGFAPVALLVSAALVALVAGAFAVVRGLDDLGGVQFGAAADPSPAGLLAESGPA
                     VGEPAAGLSGPAAAGTLHSSESAGARAVAADAPVPEPDGGDDTPPASAAAPVPYAIEH
                     RKPAPAAGLPVERPNGASERILASRIESGVRRQAGLKTSKAASAKPGAGSPRPDRPGT
                     SSVTDDKAPPASRPDSSGVSKAAGTASVTGPALSGTEGRGPGSYVVNVKPYAYVSING
                     RDYGRTPVGPIRLDPGTYKVVFTHEHRPSFSRTIVVEAGRETFETFPYPGKDEGN"
BASE COUNT         1577 a         2360 c         2184 g         1460 t
ORIGIN      
        1 cacctcggcg atggtcatca tttttcatca ccccacttcc aggaagacgg cgttccggtc
       61 gaacctccgc cagaggcctt ccgcggggcg cgtcttcgct gacgcgttcc ttcctgaaga
      121 aggcgaaccg gctgaatctg aatctcgggc acaacgagat ccagcatatc gagcagccca
      181 agaacgcgca gcactcggac aagcgtggtc agttggaccg gatcccccga ctcgaggcgc
      241 tcgagcgtcc gctttgcgat gccggccccg gaagccagtg cgtgctgcga aagaccgcga
      301 tcaatgcgat ggcgggcaat tctggtgccc agctcgcgaa gcaccgcttc gtcagtgaca
      361 gttatcgaga tcttcatgcc agtttcccaa ccatttctcg tcaaataata cgacttaaca
      421 aagaattacg caatatatcg caatttctga cgaattattg tggcggctgg ctacagtttc
      481 agcagttctt caatcgtgcg ctggatgccg gcccacagtt cgccaatcga cctcgcggtc
      541 atataggccg gggtggtgac gaccttgtgg cgccgatcca cgacgcagtc ggtcgcgggg
      601 caatcgacgt gattctggcc cagttcgcga atcttcgcgg cggtgccggg gtcgttgccg
      661 atcgtgatgt cggcgcccgt cacaccgcat tgcttaaggg tccatgctgc caggggcgga
      721 gcgatgcaga tggcgccaat cggcttgcct gccttgtgca tgccgatcag gaactcgccc
      781 actccgggat cgaccttcat cgccgcacct tcgcgcgcga atgacgacag gttgttggcg
      841 gcgccgaatc cgcccgggac gacgaccgcg tcgtaatcgg caacacgcag ttccctgacg
      901 tccacgatgt tcccgcgggc aatcctggcc gcttcgacca tgacattgcg agtctcggcc
      961 acccgctggg ccgtcacatg atccaccacc tggaactgtg gaatcgacgg cgccgcgcac
     1021 tggtactgtg ccccagccag gttcagggcc agcagggtca gcgtgttctc gtgaatctcg
     1081 ccgccatcct tgaagccgca accggccatt atcaccgcaa ccttcttcat tactggtgac
     1141 tccttgcaaa gacgggtgca ggatatcgac aaacggggcg tatgaccata tcttgatcgg
     1201 taacggcccg ccgggcagaa aattcgcccg cgctccgtaa gggcgaatga aaattcgccc
     1261 gtgttccgta agggcgaatg aaaattcgcc cgttaatgta atggtgaaaa acatgtcaaa
     1321 aatcaccagt gaattcatcg cggaaattcc gaagaccgac ctgcacgtcc acctggacgg
     1381 gtcgctgcgg ataccgacct tgattgatct tgcgcagagt gccggcgtcg ccctgccctc
     1441 atataccgac gaaggccttc gcgaactggt tttcaaggac aagtacgcga atcttgtcga
     1501 atacctgcag gggtttggcc ttacaacgtc cgtcatgacc aacgccgagc aggtcgaacg
     1561 ggtcgcttac gagctggccg aagacaactt caacgaaggc gtgcgctact tcgaggttcg
     1621 cttcgcgccc caactccatg tgacagccga tttcaacctg gatcaggtac tgctcgcggt
     1681 caacaggggg ctggcgaagg ccacctccga cttcaacgcc aggaatgccc tgattccggc
     1741 ggacgaacgc ggtccggacg gacattacgg catcatcgtg tgcgccatgc gcaacgcgat
     1801 gcccgtactg tcgccctatt ttcaggacgt cttccgggtt cacgcctaca gcaccgaccg
     1861 tgccgtcggc tcgatgacat ccctggaaat ggcccgggcg gccgtcgata tccgcgacag
     1921 gctgggaatc ccgatcaccg ggttcgatct tgccggggcc gaagccggct atcccgcagt
     1981 cgaacaccgc gacgcatttg actactgcca caagaacttc ctgaagaaga cggtgcacgc
     2041 gggcgaggcg tacggcccgg aatcgatctt ccaggcaatc accgaactgc acgcggatcg
     2101 ccttggacac ggatattatc ttctgagtcc cgacaaaatc gtgtcgcgcg ccatcgcgga
     2161 tccgcaggac tacgtccgcc gcctgtcgga gtacatcgcg gaccgccgca tcaccatcga
     2221 ggtctgcctg acgtccaaca tgcagacgaa tccgtcaatt acggacttga agcaccacgc
     2281 gttcggaaag atgctgaagg caaggctttc gacgaccctg tgcaccgata accgcctggt
     2341 gtcgaatacc accgtgaccc gcgaaatcca gcttgcggtc gatacattcg gcatcggccc
     2401 gtccgaactg aaggacatca tcgtgtatgg attcaagcgc agtttctatc acggcagcta
     2461 caacgaaaaa cggcagtacg tgcgatcggt gatcgattac tacgaggcgc tggaacgcaa
     2521 gcacggcatc cggcacgccc ccaacaccta ggcgcgcaga tgttcaagct ggtttccgat
     2581 tacaaacctg ccggcgatca gccgcaggcg atttcacgac ttgtcaacct gcttcaggac
     2641 ggcggccgcg atcaggtgct gcttggcgtg accggttccg gcaagacgtt cgcaatggcg
     2701 tccgtgattg ccgaaatcgg ccgaccggcc ctgataatgg cgccgaacaa gaccctggcg
     2761 gcccagctct tcggtgaatt caaggcattc ttcccggaga acgccgtcga gtatttcgtc
     2821 tcctattacg actactacca gccggaagcc tacgttcccg catccgacac ctacatcgag
     2881 aaggacagca agataaacga ggaaatcgac cggatgcgcc actccgccac gcgcagcctg
     2941 ttcgagcgcg acgacgtcat catcgtggcg tccgtttcct gcatcttcgg tctgggctcc
     3001 gcggaagcat attacgggct ggtggaattc ctgtctgtcg gccagcattg tgaccgggac
     3061 gcccttcttc gacgcctggt cgaacttcag tatgaccgca acgacatcgc tttcgaacgc
     3121 ggcaccttcc gcgttcgggg cgacgttgtc gaaattttcc agccgttcga ggagcaaacc
     3181 tcgctgcgca tcgtgctgga ctgcgacgaa atcgagtcga ttaccgaaat cgatccggtg
     3241 tccggccaca ggctgcgaaa cctgccaaaa gtcgccattt acccgaacag ccactacgta
     3301 accacgccgg aacgcatgcg gcgggcaatc gtcggcatcc aggacgaact ccaggaacgg
     3361 cttgtcgagt tcaaggaaag cggccgcgta ctggaggcgg caaggatcga gcagcgcacc
     3421 ctttccgata tcgaggcact tgagcagttc gggcgcgtcg ccggaattga aaactactcc
     3481 cggcacttga ccggccgcgt cgcgggcgag gctcccccga ctttgcttga ttattttcca
     3541 aaggacttcc tgtgcttcct ggacgagtcc cacatgaccg tgcctcagct tcacggcatg
     3601 taccgcggcg accgcgcccg aaaggacgtt ctcgtgaact acggattccg ccttccgtcg
     3661 gcggtcgata accgcccgct gcgattcgac gagttcgatg aacgccgggg acggacgatt
     3721 tacgtgtcgg caacaccggg cgacttcgaa atgaaaagat ccggtgctgt cggccctgac
     3781 ggctccccgg atccaaattc cgagtccggg cggatcgcgc ggatgatcgt ccgcccgacc
     3841 gggctgctcg accctgtaat cgatgtgagg cccgcgacga accaggtcga tgacctgctg
     3901 gacgaaatcc gggccgtggt cgatcgcggc cagaggatcc tggtaaccac gctgaccaag
     3961 aaaatggccg agcatctgac cgaatacttc accgaggcag gactgaaagt caattatctc
     4021 cacagcgacg tgcacacgct tgaacgcatt cagataatca aggatctgcg cgccggcgtc
     4081 ttcgacgtcc tggtgggcat caacctgctt cgcgagggcc tcgaccttcc tgaagtcgcc
     4141 ctggtcgcga tccttgacgc cgacaaggaa ggtttcctgc gaagcgagcg atccctgatc
     4201 cagacgttcg gccgcgccgc acgcaacacc gatgcgcttg tcatcatgta cgccgacacg
     4261 gtcacgcgat ccatgcaggc ggccatggac atcacgacgg ccaggcgcca gcgccagatc
     4321 gaattcaacg aaagacacgg gatcacgccg acaacggtcg cccgccgcct gttcttcgtc
     4381 gaggaggccg aacatgctgc cgtggttgcc ggcgtaaagg atcccaacgc gctttcggat
     4441 ctggtgatgg atccgaagtt gctcggcaag accttgaatc aattaaaaaa gcagatgctc
     4501 gaggcgtccg cgaaactgga atttgaacga gctgcggaaa tacgcgaccg cattcgagca
     4561 atccgcaatg tgatgggaaa tctttaacgg gcctctggcc ggggagttgt tgaaagatgc
     4621 ttgtcaggaa gaaaggaacg tcggagcaca ttccggtcat gatgaccgga gcaagcaagg
     4681 tgtcaaaaca gatcacggtt ggcagggccg atggaacgcc cgcctattcg atgagaatct
     4741 tcaccgtgga gccgggtgga cacactccat ttcactcgca cccgtttgac cacgtcaact
     4801 tcgttgtatc cggaagcggg gtcctggttt ccgccggcgg acagacgccg gtcgaggctg
     4861 gcgacagcgc cctggtgctg cccgatgaga tgcaccagta ccgcaacacc ggcagtgaac
     4921 ctttcgtttt catctgcttc gttgacaagg catacgaata gccgggcaac gcaaccgtcg
     4981 catgggcgta cggcgtggcg gtggttgcag ggtctgaaag aatcggttcg ggatcaatcc
     5041 tcgcggggct gatcgcagtc gagttcaaca acctgatcgc ggaaaagaac cgtcatcgag
     5101 tattccacat cgtcacggac aacgacgcca aggccgtgga ccttgtgggt gatgacgtcg
     5161 tccttgtgat agctgcgatg cttgtcatac accttcattt tcattggact gacattggcc
     5221 gtggcttcgt cccacaggcg gcgcatgaca gcctccggcc ttaaatcagc ggctgaagcc
     5281 tttgccgcgg cgtccttttt cgccttggcc tcggccgccg cgggaccacg cgtatgcttc
     5341 tcggcgattt tcatcgcctt ctcgaccagc ttacgatgct tttcctgcag gtcggccggc
     5401 ggcttgaagt cctggaaatg catgcacgtg cggcactgaa ccttggccag ggtaccgtcg
     5461 gggttcatcg acgcgaccac ggcatccagg ttcaacctgc aatagcggca gtagatgtcg
     5521 atgaactggc caagcttgta tgacggatta tcggacattg aagcctcctg gcgggcgaat
     5581 tttcattcgc ccctacaggc cccgcagcgg ctgaacccag acctggcgcg tgcgcggccc
     5641 gtcgaactcg gcaaaaaaca cgtgctgcca cgtaccaagg gacatcttac cgccaaccac
     5701 aggaatcgtc agcgaattcc ccatcattat cgacctgacg tgggcatggc tgttgccttc
     5761 ggcgtgaagg tattccgtgc agaacggata gattctttca agggtactct cgacatccct
     5821 gacgacgtcg gggtcggcac cctcgttaca gaagataccg gccgtcgtat gcggacacca
     5881 cacaagaacc gcgccctgat caattccaga acccgtcaag acttcggcga cacgatccgt
     5941 gatatcgatg gtctggatcg gacgaaccgt gcggacttca aaactgttca cgacaaaacc
     6001 ccaacgaact ttccggcgca tgttatcgca aaaccggacg cggcggtcaa tcgcttgcca
     6061 caacgacacg gttttgtggt aaaagatgcg cgtcacattt ttccgttcag gcgggggacc
     6121 gacagatggc agatgatcgc gcgacatcgg gtgtcactga agccgaaacc atcgatcttg
     6181 acaacattca tacagatcac actccatccg accttttcaa ggtcggcttc gtctatgggc
     6241 ttggtggtgg cggtgtgacc gcaatcatcg gcgtcagatt cctggttttt ggcaacggcg
     6301 cacaggaaca cgcggccgga atcatgttct tcgtcgtttc ggggttgatg ctcttcgccg
     6361 gtgtctggtg tcacgtgaag cgattccgga tgctccgaaa caaggcttga ttaatttcct
     6421 tcatccttcc ccggatacgg aaaggtctcg aaagtctccc gcccggcttc aaccacaatc
     6481 gttctggaaa acgacggcct gtgctcgtgc gtgaaaacca ccttataggt tcccggatca
     6541 agccgaatcg gaccaaccgg cgtccgaccg tagtcgcgtc cgtttatcga cacgtaagcg
     6601 taaggcttga cgttgaccac gtacgaacca ggcccgcgcc cttccgtgcc tgacagggcc
     6661 gggcccgtga cggacgccgt tccggcggct ttcgacacac cgctggagtc gggccgggaa
     6721 gccgggggtg ccttgtcatc ggtcaccgaa gacgtcccgg ggcggtctgg cctgggactt
     6781 ccggcacccg gcttcgcaga ggcagccttg gaggttttca atccagcctg acgccgaaca
     6841 cccgattcaa tccggcttgc caggatgcgt tccgacgcac cgttgggccg ttcgaccggc
     6901 aggccggcag cgggcgccgg tttgcggtgt tcaatcgcat aagggacagg tgcggccgct
     6961 gacgccggtg gtgtgtcatc cccgccatca ggttctggaa ccggcgcatc tgctgcaacg
     7021 gccctggctc ccgcagactc ggacgaatgc agcgtccctg cagcggccgg cccggaaagt
     7081 ccggccgcgg gttccccaac ggccggcccg ctttccgcga gcaggcccgc cggcgacgga
     7141 tcggccgccg ccccgaattg aacgccgccc aaatcatcaa gcccgcggac aactgcaaac
     7201 gccccggcca cgagcgccac cagcgccgca gacaccagca aagcgactgg ggcgaatccc
     7261 cgccgtcggg cacgcagctg cgggcgatct tctttctttt cgctgagttt ggccgcgacg
     7321 ggactgagat ccagagcatc gacaatcgaa gcgtccgaac cggccgcgcc gacagggtct
     7381 gcagttgaga cgggtgccgg caccgattcg tcccctctgg caccccgcac ttcaagaaca
     7441 gtcgggccat ctgactgctc cgcggaaatc gccgttgaca tggcctcgac cgcgtcgtga
     7501 agcgcgtcgg caaatccgtc catatccgga aagcgatccc cgggctcggg cgacaatgcc
     7561 ttgaaaagcg cgccgtgaag c
//
LOCUS       DAOPPA010000098         7507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10936_length_7507_cov_4.171900, whole genome shotgun sequence.
ACCESSION   DAOPPA010000098 DAOPPA010000000
VERSION     DAOPPA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10936_length_7507_cov_4.171900"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1165
                     /gene="nagZ"
                     /locus_tag="PKH54_04380"
     CDS             176..1165
                     /gene="nagZ"
                     /locus_tag="PKH54_04380"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOC99158.1"
                     /translation="MKPDVDSLDDQLVASLFVIGFDGYELPRQTAELLGGGLAGVTLF
                     RRNIADRIQLTELCLSIREAARPSGLVPIIAVDQEGGRVQRLRHVGRLYDAARDQDDP
                     ADAGRRIGSELALMGFNVDFAPVLDVDSNPANPIIGDRAFSADPEVVASSAVAFHDGL
                     VSAGVMGCGKHFPGHGDASADSHLELPVIRAGLDTLQARELIPFTAAVEAGFKMLMTA
                     HCLYPLVDANFPATLSPCFIKPILRGSMGFDGVVISDDLGMKAISDRFSPGDIVRLGV
                     DAGLDIFLHCGIDGEALQLIEALRDQLDSGVIPACDVVAAAARIRTLRSRFLR"
     gene            complement(1143..>7507)
                     /locus_tag="PKH54_04385"
     CDS             complement(1143..>7507)
                     /locus_tag="PKH54_04385"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99159.1"
                     /translation="ICDETTGCGVLPLTGGACDDENACTENDTCTNGVCGGSVVVCPQ
                     DTECSHFWCDTTLGCQEDWLTASCDDGDPCTVGDACIMGVCGAGGFMNCNDGNPCTTD
                     SCEDGICKHPKVANLTQCDDGNPCTQDDFCFNGYCVGATGLNCDDEDPCTTDSCDLET
                     WTCIYDANNNACDDSNKCTTNDQCSNKQCSGTPISCDDNNPCTTDSCNPSTGCVHVNN
                     TAECEDGNLCTDGDVCSGGSCQPGGSVDCDDDNVCTTDSCLPSTGCKHDHAPGPCDDG
                     DLCTYSDLCINKTCVGTPRTCEDPLPCTDAACNGTEECSLTILPDWCVINGQCVTAYT
                     TQAGNPCRVCDPLASQNSWTNADGATCDDGQDCTKLDTCADGTCAGQPYDCNDNLPCT
                     PDACDGDGTCTNEPLAGWCVISDTCFADGAVNPDNQCQACHSGINPRSWGNVTGPCSD
                     GNECTVDDSCVDGACQQGLPRDCDDDNVCTLDSCESDLPGGCVHQATPAICATARCEG
                     LVLYPAMMCQGESCPEQQPVNCDDDNECTDDQCTLLGCVNTPNTDPCDDGNPCSRNDA
                     CNNSSCGGTPYSCPVSNECILSSVCDGLGGCTETARDAGTPCTDDNNPCTADECDGLG
                     ACGHDPVEDDTPCDDFNLCTTGDKCLGGVCSDGNDVQCEPLDQCHVAGECDPMTGECS
                     NPPAENGTTCDDGNPCTFDDQCLEGACTGGTQNPCTPPDDCHGAGTCDPLTGTCTYPT
                     LEDGTTCNDGNLCTQRDICSKGVCKGLDPITCTVISPCHYRGTCEPSTGVCSTPLRPD
                     GTTCDDSNACTTGTSCTEGVCTGGTTKTCPAQNNCHEDGVCNTVTGVCSVVRKDDGAP
                     CTDFNQCTSADQCLNGSCVGLSYTCYDGKSCTTDTCKGDGTCSFPVNAGWCLIDSYCR
                     PEFQQNPSNVCEICNPATNQSAWSGNSGAPCNDFRLDTKNDACVDNVCVGTPYDCSDG
                     LECTEDVCDGNGGASHSLLSGYCLIDNACYANNQVNPADPCQRCSVAASTSAWSPVSN
                     GLQCDDSEQCTYNDKCQDGVCSGTAYSCEDGLECTDNVCNGNGNCGGSVQPGWCVIWN
                     TCVPSETGNPMNSCQKCDPAAAVLGWSPANQGQPCNDNNPATTDDVCIDGNCIGQGVS
                     CVDGLECTQDIPGENGCENLLYPGWCLIDNTCIFDGNTNNENRCQICNAAIAQFNWSA
                     NDGQACSDNDACTSDDHCSNQECVGTMMTCNDGISCTTDSCSGGVCEFRIDTGWCLID
                     GQCVAEGTVNPVAECEKCDPDILKVNWSSNEGVQCDDSNPCSHNDACRAKVCRGVEYG
                     CDDGIACTTDICDGSGGCQRPVLEGWCLIDGNCMTEGTTSMFNSCLKCSPSESSRDWS
                     FNDGAACDDNDSCTWPDSCQQGACDGVPKPCDDGLACTTDRCDGNGGCLAPELKSGWC
                     LIDGACYMNLQPNPSDPCLICKANMLAHDWSFRDGVACDDTNDCTIFDTCLSGSCQGT
                     PYSCDDGRSCTLDACDGGGGCNSDFIIDGTCLIEGMCRVTAEPNPAVQCQGCLPIASR
                     TEWSVMPGGPCSDLNDCTFNDTCDMLGACGGTANPCDDSIACTDDVCDGNGGCSYPIS
                     RDWCLIGGNCVPDGSPHPTESCSVCAVGVSQTDWTFQDSQNPEICNGADDNCDGITDP
                     ENAPGCMMHYLDHDGDGAGIPGTSRCLCAPDGEFRSLNADDCDDNNAAVYGGKTEVCD
                     GFDNNCDGDTDEQDASDCTVYLRDGDRDGFGQSGSERCLCAPEGTHDATVSGDCNDDN
                     ADVNPNENEICNTVDDDCDGLTDPDGSTGCQLRYRDEDGDGYGVGPAICMCSPRGVYT
                     LTSEGDCDDLDYYANPLGVESCNGKDENCDGITDNADVGIACPLDEGFDVNGTITCGA
                     DGCGLACQTRTITPPATPWHDTNGYFGDGCECEGDGYDGIGGATCQNAVVLPDLQDSG
                     QNVVIEGNLPAQGAVDWYKVTFIDDTWNSETSGGDHFHADIQLKPEDLEEFEFAVFDG
                     ACDGTSMCSAGSHFEWAVDFWTSTLGEKPCGLVGANCVDGNPKDYQQCLAVTGDPERC
                     GSCPALPGPQMHMCTNNSRTVYVRVKRHDGLGASCKSYSLTLRNGI"
BASE COUNT         1261 a         2265 c         2316 g         1665 t
ORIGIN      
        1 tggcggcgac gctgcttatc gcatggttcc tttattcata ctgggccagt tccgaggtgt
       61 atcttggaat cctggatcgt caggcgccgc aaatctgcca ggttattggc gatgccttcg
      121 ggccgggcgg cagaaccgcc tgaattacct tccttccgtg actccggaat gttcgatgaa
      181 accagacgtg gacagccttg acgaccagct tgttgcttcg ttgttcgtaa tcggctttga
      241 cggttatgaa ctgccccgcc aaaccgccga actgcttggc ggcgggcttg ccggcgtgac
      301 tcttttccgg cgcaacatag cggacaggat tcagttgacc gaactgtgtc tttccatccg
      361 ggaagcggcc cgcccgtcgg gacttgtgcc aatcatcgct gtcgatcagg aaggtggaag
      421 ggttcagcgt ctgagacacg tcggtcgatt gtatgatgcg gcccgcgacc aggacgaccc
      481 ggccgatgcc ggaaggcgca ttggctccga actcgccctc atgggcttca acgtcgattt
      541 tgcgccagtg ctcgatgtcg attcgaatcc ggccaatccc atcattggcg atcgggcatt
      601 ttcagcggat cctgaagtcg ttgcatcaag cgcggtggct tttcatgatg gcctggtcag
      661 cgccggcgtc atggggtgtg gcaagcactt tcccggtcac ggcgatgcct cggccgattc
      721 gcatctggaa ctgccggtga tccgggcggg cctcgatacc cttcaggccc gtgaattgat
      781 tccgttcacg gcggccgtcg aggcaggttt caagatgttg atgaccgccc attgccttta
      841 tccgcttgta gatgccaatt ttcctgcgac cctttcgccg tgcttcatca agccgattct
      901 tcgtggttcc atgggattcg acggagtcgt gatatccgac gatctgggaa tgaaggcgat
      961 ttccgaccgc ttcagccccg gcgacatcgt gaggctggga gtcgatgccg gcctggacat
     1021 cttcctgcat tgcgggattg atggcgaggc cctgcaactg atcgaagcgc ttcgcgatca
     1081 gctcgattca ggcgtcattc cggcgtgcga tgtggtcgct gcggcggcaa gaatccgaac
     1141 gctcagatcc cgtttcttaa ggtaaggctg tacgacttgc aactggcgcc aagaccgtca
     1201 tgccgcttga cccgtacata caccgttcgg ctgttgttcg tgcacatatg catctgcggt
     1261 cccggcaggg cggggcagga gccgcatctt tccggatctc ccgtgacggc gagacactgc
     1321 tggtagtcct tcggatttcc atcgacgcag ttggcgccca cgagtccgca cggcttctcg
     1381 ccaagagttg aagtccagaa atcgacggcc cattcgaaat ggctgcccgc actgcacatg
     1441 ctggtgccgt cgcacgcgcc gtcgaatacc gcgaattcaa attcctccag atcctcgggc
     1501 ttgagctgga tgtcggcgtg gaaatgatcg cctccggagg tttccgaatt ccatgtgtcg
     1561 tcgatgaacg tgaccttgta ccagtcaacg gcgccctgcg ctggcaggtt accttcgatc
     1621 acaacgttct ggcccgaatc ctgaagatcc ggcaggacaa ccgcgttctg gcatgtcgcg
     1681 ccaccgatgc cgtcatatcc gtcgccttcg cattcgcaac catcgccgaa atagccgttc
     1741 gtgtcatgcc acggcgtggc cggcggggtg atggttcttg tctgacaggc cagtccgcag
     1801 ccatctgcgc cgcatgtgat cgttccattg acatcgaaac cttcatccag ggggcaggca
     1861 atcccgacat ccgcgttgtc ggtgatgccg tcacagttct cgtcctttcc attgcaggat
     1921 tccacgccga gcggattggc atagtagtca aggtcgtcgc agtccccctc ggaagtcagg
     1981 gtatatacgc cgcgcgggct gcacatgcag atggccgggc cgacaccgta gccgtcgccg
     2041 tcctcgtcgc ggtaccgcaa ctggcaaccg gttgaaccgt cgggatccgt cagtccgtcg
     2101 caatcgtcat cgacggtgtt gcagatttca ttttcattcg ggttcacatc ggcattgtcg
     2161 tcgttgcagt cgccggaaac agtcgcgtcg tgcgtgcctt ccggggcgca caggcatcgt
     2221 tccgagcccg actgcccgaa gccatccctg tcaccgtccc ggagatagac cgtgcagtcc
     2281 gacgcatcct gttcgtccgt gtcgccgtca cagttgttgt caaaaccgtc gcacacttcg
     2341 gtcttgccgc cgtaaaccgc cgcgttgtta tcgtcgcagt catccgcgtt cagtgaacgg
     2401 aattccccat ccggcgcgca caggcacctt gaagttccag gaatgcccgc gccatcgccg
     2461 tcatgatcca gatagtgcat catgcatccg ggcgcgtttt ccgggtcggt gattccatcg
     2521 cagttgtcat cggcaccgtt gcatatttcc ggattctggc tgtcctggaa agtccagtcg
     2581 gtctggctta ccccgacagc gcacacgctg caggattcgg tcggatgcgg ggatccgtcc
     2641 gggacgcagt ttccgccgat caggcaccaa tccctggaaa tcgggtacga gcatccgcca
     2701 tttccgtcgc agacatcgtc agtgcacgcg atcgagtcat cacaggggtt tgcggtgccg
     2761 ccgcaggcgc cgagcatgtc gcaggtgtcg ttgaaggtgc agtcgttcag gtcgctgcac
     2821 gggcctcccg gcattactga ccattcagtc ctgctggcga tcggcaggca gccctggcat
     2881 tgaaccgccg ggttcggctc tgctgtcacg cggcacatgc cttcaatcag gcacgtcccg
     2941 tcaataatga agtccgagtt gcagccgccg ccgccgtcgc acgcatcaag cgtgcatgaa
     3001 cggccatcgt cgcaactgta gggggttccc tggcagcttc ccgaaaggca cgtatcgaat
     3061 atcgtgcagt cgttggtgtc gtcgcaggcc acgccgtcgc ggaaagacca gtcgtgagca
     3121 agcatgttcg ccttgcagat caggcagggg tcggacgggt taggctgaag attcatgtag
     3181 caggcgccgt caatcaggca ccaacccgac ttcagttcgg gggcaaggca cccgccgttg
     3241 ccgtcacagc ggtcggtcgt gcatgccagg ccgtcgtcgc aaggcttcgg gacgccatcg
     3301 cacgcaccct gctgacagga gtccggccat gtgcacgaat cgttgtcgtc gcacgccgcc
     3361 ccgtcgttga acgaccagtc gcggcttgat tcggacgggg aacacttgag gcaactgttg
     3421 aacatgctgg tggtgccttc ggtcatgcag ttgccgtcaa tcaggcacca tccttccagg
     3481 accggccgct ggcacccgcc gcttccgtca cagatgtcgg tggtgcaggc gatgccgtcg
     3541 tcgcatccat attcgacgcc gcggcagacc tttgcccggc aggcatcgtt gtgcgaacac
     3601 gggtttgaat cgtcgcactg gacgccctcg ttgctgctcc agttgacctt caggatatcc
     3661 gggtcgcatt tctcgcattc cgccacagga ttcacggtgc cttcggccac gcactgtccg
     3721 tcgataaggc accagcccgt gtcaatccgg aactcgcaga cgccaccgga gcacgagtcg
     3781 gtcgtacacg aaattccgtc gttgcaggtc atcattgtgc cgacacattc ctggttcgaa
     3841 caatggtcgt cgctggtgca tgcgtcgttg tcgctgcaag cctgaccatc gtttgccgac
     3901 cagttgaact gggcaatcgc ggcgttgcag atctggcacc tgttttcgtt gttggtgttt
     3961 ccgtcaaaga tgcacgtgtt gtcaatcagg caccatcccg ggtaaagaag gttctcgcag
     4021 ccgttttcgc cggggatgtc ctgggtgcat tcaagtccgt caacgcatga aacgccctgc
     4081 ccgatgcagt tgccatcgat gcagacgtca tcggtcgtcg ccgggttgtt gtcgttgcaa
     4141 ggctgtccct ggtttgccgg agaccagccg aggacggccg ccgccgggtc gcatttctgg
     4201 caggagttca tcggatttcc ggtctcggaa ggcacgcagg tgttccagat aacgcaccag
     4261 cccggctgga cggaaccacc acagttgccg ttgccgttgc acacattgtc cgtgcactca
     4321 agaccgtcct cgcaggagta ggccgttccg gagcatacgc cgtcctggca cttgtcgttg
     4381 taggtgcact gttccgagtc gtcgcactga agcccgttgc ttaccggcga ccaggctgac
     4441 gtgctggcgg cgaccgagca gcgttggcag ggatcggccg gattgacctg gttgtttgca
     4501 tagcaggcgt tatcgatcag gcagtacccc gacagaagtg aatgagatgc cccaccgttt
     4561 ccgtcgcata cgtcttcggt gcattccagt ccgtcggagc agtcgtaagg cgttccgaca
     4621 cagacgttgt cgacgcaggc gtcgttcttc gtgtccaggc ggaagtcgtt gcacggggcc
     4681 ccgctgtttc ctgaccaggc cgactgattc gtcgccgggt tacagatttc gcagacgttc
     4741 gacggattct gctggaattc cggcctgcag tacgaatcga tcaggcacca gccggcgttg
     4801 accgggaagc tgcacgtccc gtcaccctta caggtatccg tggtgcacga ttttccgtca
     4861 tagcaagtgt aagacaggcc cacgcaagat ccgttcaggc actggtcggc gcttgtgcac
     4921 tggttgaagt cggtgcaggg cgcgccgtcg tccttgcgaa ccactgaaca cacgccggtc
     4981 acggtgttgc agacgccgtc ctcgtggcag ttgttctggg ccgggcacgt ctttgtggtg
     5041 ccgcctgtgc agacgccctc cgtgcatgac gttccggtcg tgcaagcgtt gctgtcgtcg
     5101 caggtcgtgc cgtccggacg caacggggtc gagcagacgc cggtcgaggg ttcacaggtt
     5161 cccctgtaat ggcatgggct gatgactgtg catgtgatcg gatccaggcc cttgcacacg
     5221 cccttcgagc atatgtcgcg ctgggtgcac agatttccgt cgttacatgt ggttccgtct
     5281 tccagggtcg ggtacgtgca ggttcccgtc aacgggtcgc aagttcccgc gccatggcag
     5341 tcgtcgggcg gcgtgcacgg gttctgtgtt ccaccggtac aggcaccttc aaggcactgg
     5401 tcgtcaaacg tgcacggatt cccatcgtcg cacgtcgtgc cgttttcggc cggcgggttg
     5461 ctgcactcgc ctgtcatcgg atcgcattcg ccagcgacgt ggcactgatc cagaggctcg
     5521 cactgcacgt cgttgccgtc ggaacatacg ccgcccaggc atttatcccc ggtcgtgcag
     5581 aggttgaagt cgtcacaggg cgtgtcgtct tcaacggggt cgtgaccgca ggcgcccagg
     5641 ccgtcgcatt cgtcggcggt acacgggttg ttgtcgtccg tgcacggagt cccggcatcc
     5701 cgggcggttt cggtgcatcc gccaagtccg tcgcagacgc tcgacaggat gcattcgttt
     5761 gaaaccgggc aggagtaggg cgtcccgccg caggaagaat tgttgcatgc gtcgttcctg
     5821 gagcatgggt tgccgtcgtc gcacggatcc gtgtttggcg tgttgacgca tcccagcagg
     5881 gtgcattgat cgtcggtgca ttcgttgtcg tcgtcacagt tgacgggctg ttgttccgga
     5941 caggactcgc cctggcacat catcgcggga taaagcacca atccttcgca cctggcggtg
     6001 gcgcagatcg caggggttgc ctggtgaacg catccccctg gaagatcaga ttcgcacgag
     6061 tcaagagtgc agacgttatc gtcgtcgcag tcccgcggca gtccctgctg gcaggctccg
     6121 tcaacgcagg agtcatcgac cgtgcactcg ttgccgtcgc tgcatgggcc ggttacgttg
     6181 ccccacgatc tgggattgat gcccgaatgg caggcctggc actggttatc ggggttgacg
     6241 gcgccgtcgg cgaagcatgt gtcggaaatc acgcaccagc cggcaagcgg ctcgttcgtg
     6301 caggttccgt ccccgtcgca ggcatcaggc gtgcagggca ggttgtcgtt gcagtcgtac
     6361 ggctgcccgg cgcaggttcc gtcggcacac gtgtccagtt ttgtgcagtc ctgtccgtcg
     6421 tcacacgtcg cgccgtcagc gttggtccac gaattctggc ttgccagcgg atcgcagacg
     6481 cggcagggat ttccggcctg ggtggtgtag gcggtcacgc actggccgtt aatcacgcac
     6541 cagtccggca ggatggtcag cgaacattct tcggttccgt tgcatgcggc gtcggtgcag
     6601 ggcagcgggt cttcgcatgt cctgggagtg ccgacgcatg ttttgttgat gcagaggtcg
     6661 gaataggtac acaggtctcc gtcgtcgcat ggtcccgggg catggtcgtg cttgcagccc
     6721 gtggatggca ggcacgaatc ggtcgtgcag acgttgtcgt cgtcgcagtc aactgatcca
     6781 ccgggctggc acgacccgcc agaacagacg tcgccgtccg tgcaaaggtt tccatcctcg
     6841 cattcggcgg tgttgttgac gtgcacgcag ccggttgacg gattgcacga gtcggtcgtg
     6901 cacgggttgt tgtcatcgca ggaaatcggt gtcccggaac attgcttgtt ggaacactgg
     6961 tcgtttgtgg tgcatttgtt ggagtcatcg caggcgttgt tgttcgcgtc gtaaatgcag
     7021 gtccacgttt caaggtcgca cgaatccgtc gtgcagggat cctcgtcgtc gcagttcagg
     7081 ccggtggcgc caacgcagta cccgttgaag cagaagtcgt cctgggtgca gggattgccg
     7141 tcatcgcact gggtcaggtt tgccaccttc gggtgtttgc agatgccgtc ttcgcaggag
     7201 tcggtcgtgc acgggtttcc gtcgttgcag ttcatgaacc cgccggcgcc gcagaccccc
     7261 atgatgcagg cgtcgccgac cgtgcaggga tccccgtcgt cgcatgacgc ggtgagccag
     7321 tcctcctggc agccgagtgt cgtgtcgcac cagaagtgcg agcattccgt gtcctgtgga
     7381 cagaccacaa ccgagccgcc gcagacgccg ttggtgcagg tgtcgttttc ggtgcatgcg
     7441 ttttcgtcgt cgcacgctcc gccggtcagc ggaagaactc cgcatcccgt ggtttcgtcg
     7501 catattg
//
LOCUS       DAOPPA010000099         7493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10956_length_7493_cov_4.141974, whole genome shotgun sequence.
ACCESSION   DAOPPA010000099 DAOPPA010000000
VERSION     DAOPPA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10956_length_7493_cov_4.141974"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PKH54_04390"
     CDS             complement(<1..1161)
                     /locus_tag="PKH54_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOC99160.1"
                     /translation="MIKSYHEPLTFDQIKAGEGLKAALKMTRSEVIKAISDSGMRGRG
                     GAGFPTGVKWNLCAAAAAPEKFVVCNADEGEPGTFKDRVLLAEYSDKVFEGMTIAGYA
                     IGAKEGIIYLRAEYMYLRAELEAALAKRREDKLLGDKIKGAKEFSFDIRIQMGSGAYV
                     CGEETALIESLEGHRGEPRNRPPFPVDTGFKGCPTSVNNVETFAWATCILAKGPKWFK
                     ELGTEKSAGLKLFSVSGDCDQPGIYEFPMGITVADLLVAVGGEGAKAVQIGGASGHCV
                     PYWEFDRKICFEDVATGGSIIVFDFDRDMLEVAMNFMEFFCEESCGQCTPCRDGNSAI
                     LEGLEMLEEGVCSQSYLKELCELGETMQLASKCGLGQSSPNAFLSIVKNFKDE"
     gene            complement(1158..1676)
                     /locus_tag="PKH54_04395"
     CDS             complement(1158..1676)
                     /locus_tag="PKH54_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOC99161.1"
                     /translation="MLVSEQDVLKQDIAAWVKHYGKDRCYLIPILQEIHRKYHQCSEF
                     AMQVVADELDIHPVEVYSVVSFYSFLSHDTHGKYVVRLCRTISCDMAGKDQIANQLVS
                     DLGVDFGETTPDGKFTLEWTNCLGACDQGPAMLVNETLYVGLTPEKVHEILETCRRTF
                     GVYALQNKEGHA"
     gene            complement(1681..2085)
                     /locus_tag="PKH54_04400"
     CDS             complement(1681..2085)
                     /locus_tag="PKH54_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC99162.1"
                     /translation="MGAEARESLVARILIVDDDPDVVDACTLILEKNGYEVASANNVT
                     DGKAQIQKVNPDLLILDVMMEQPDDGIVMAQDLRRVGFAKPILMLTSISRVTGMSFGS
                     DSDLVPVDAFQEKPIPPKKLLETVAELLHKEK"
     gene            complement(2472..2675)
                     /locus_tag="PKH54_04405"
     CDS             complement(2472..2675)
                     /locus_tag="PKH54_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99163.1"
                     /translation="MDSVAVTRIGKLLIIHIDNPSVPTIRERVFALLNGAGRLPVSGI
                     IEAWAGDAPDEFHWKTVIGVYAA"
     gene            complement(2878..4662)
                     /locus_tag="PKH54_04410"
     CDS             complement(2878..4662)
                     /locus_tag="PKH54_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOC99164.1"
                     /translation="MKLHLIPVVLISILLVAGCGGDSVQGDLDGAPDALGTDNPRLDV
                     NTPGDDVVPEDAGLDLPGQDAVESDGGGDSTEPVDVVDPDSSFDSDVQVVSTEVNYLV
                     IASDSLLESASELAEYRQSRGFRTQVMTRSDILGGTTVSTANWTNMARAHINKLFKKL
                     PAEETLFVVLVGDVEDVPEEGFTKPLPTTDCDNVLTGICFTDNSIADLDDDYIPDVAL
                     GRITASNNQEVRDYLQKVVNHEAGYTPGLWNRRVSLYVGEAGFSPEIDATLEYFTFQG
                     LDLISHAFDIIGAYDSENSSYYYTPFHEKVVELINQGSLMSIYIGHGNEDWTQGLKTD
                     QLPEIKCENRLPVMVFLACLNGNFDWGDDSIAEAMMHLPDGPVAVFASSDESHPVGNA
                     VLAYEVTRVGLGERPKTAGELFMRTKAELINHSDDFRQTIDGASIAYGEQGADDPERL
                     YREHADLYNLLGDPAVMMKYPPASVSSLDVNGTLAGKQLTVSGTVAGFADGRAYVTLE
                     VSRNEFAGELIQDPESDAEIQANWEIANDKVVAAETVDVMSGVFVADLSWDRAIGGGD
                     RYIKVYAWDGSVLGQGTVDAIGEKRINK"
     gene            complement(4850..5191)
                     /locus_tag="PKH54_04415"
     CDS             complement(4850..5191)
                     /locus_tag="PKH54_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOC99165.1"
                     /translation="MSLFRKALAAVGATFVSMLVLVPSAFAQEAVAAGDNKFTVQVFV
                     ALAAGFGIAIAAFGGAMAQGKAVTAAMEGIARNPGAQGKMFVPMILGLALIESLVIYS
                     LVISFSLIGKL"
     gene            complement(5258..6160)
                     /gene="atpB"
                     /locus_tag="PKH54_04420"
     CDS             complement(5258..6160)
                     /gene="atpB"
                     /locus_tag="PKH54_04420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOC99166.1"
                     /translation="MKFRFASSLTALAIVLLSGSVPASASDDHWSLASEVIPADVTDA
                     VAHGLGRTLIGKEPVTDLNHVVFGVLVFAILVVLMVLAAVKMRSGRGSVIPEARFSPL
                     ALFQFLSDALLGLMEGLMGREKALYFLPLIGSLGIFILFSNLLGLIPGFLPPTSNLNT
                     TLALGLVVFFATHYYGVKTHGIGYFKEFFGPIIKWYALPLMLLMFCIEMISHIARPVS
                     LAIRLFGNMFGDHAALAAFLLMAAGTLAGGPWIARVSVVMPLIPMVLGIFVAVVQTLV
                     FCILSTVYIYMATEEHGEGDAHHA"
     gene            complement(6200..6574)
                     /locus_tag="PKH54_04425"
     CDS             complement(6200..6574)
                     /locus_tag="PKH54_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99167.1"
                     /translation="MTARRFVRNTVCVNAVLAAVAGAVSLAVAGKGPGFAALAGGLAG
                     SLNVLVIGLIAMRMFSPGAGRASTVALLLVKMGVLAGLALLAVGILSVSPVGLAIGVS
                     TTVVAVLSMSCFVALRGDGVTL"
     gene            complement(6584..6829)
                     /locus_tag="PKH54_04430"
     CDS             complement(6584..6829)
                     /locus_tag="PKH54_04430"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOC99168.1"
                     /translation="MASNRKKGPLGSAVAVMSVGIEMAIAITVGYFIGNWIDGKLDTA
                     PWFLLLFLALGSAAAFRGLWRTARKYWAGDDEGPGSV"
     gene            complement(6998..>7493)
                     /locus_tag="PKH54_04435"
     CDS             complement(6998..>7493)
                     /locus_tag="PKH54_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99169.1"
                     /translation="VSPQAEPQAAPQAAPKPEAFPISIHAYPPAVQIQVDGRRVGTGR
                     VEGLMLEPGRHRLVLSHPSCDQCRDVDTSFLIDRESPPKAPMRVSIDYREARLLVVGP
                     QNGKVFLNNEARPRGVTNQPIMVPMDRPTPVDFMLKVVVEGRPDFSTRVSLQAGKAAA
                     VRLQ"
BASE COUNT         1643 a         2267 c         2177 g         1406 t
ORIGIN      
        1 ctcatccttg aaattcttga cgatggacag gaatgcgttc gggctggact gtccaagtcc
       61 gcacttcgac gcaagctgca tcgtctcgcc aagttcgcaa agctccttca ggtagctctg
      121 ggagcagacg ccctcttcca gcatttcaag gccttcgaga atcgcgctgt taccatcgcg
      181 gcacggtgtg cactgaccgc agctttcctc gcagaagaac tccatgaagt tcatcgcgac
      241 ttccagcatg tcccggtcga aatcgaaaac gatgatggaa ccaccggtgg ccacgtcttc
      301 aaaacagatc ttgcgatcga attcccagta tggaacgcag tggccggaag cgccgccaat
      361 ctggacggcc ttcgcgcctt cgccaccaac ggccaccagc aggtcggcaa ccgtgatccc
      421 catcgggaat tcatagatgc ctggctggtc gcagtcgccg gaaaccgaga acagcttcag
      481 gccggccgac ttttcggtgc caagttcctt gaaccacttc ggacccttgg ccaggatgca
      541 cgtcgcccag gcgaacgtct cgacgttgtt gaccgacgtc gggcagccct tgaatccggt
      601 atcgaccggg aacggcggcc tgttgcgcgg ctcgccgcga tggccttcca gggattcgat
      661 aagcgccgtt tcctcgccgc agacgtacgc gcccgaaccc atctggatgc ggatgtcgaa
      721 cgagaattcc ttggcgccct tgatcttgtc gcccaggagc ttgtcttccc gacgcttcgc
      781 cagggcggct tcaagttccg cgcgcaggta catgtattcg gcgcgcagat agatgatgcc
      841 ttccttcgcc ccgatggcgt aaccggcaat cgtcatgcct tcaaagacct tgtcgctgta
      901 ttcggccagc agcacgcgat ccttgaacgt tccgggctcg ccttcgtccg cgttgcagac
      961 cacgaacttt tcgggagctg ccgcggccgc gcaaaggttc cacttcacgc cggtcgggaa
     1021 gccggcgcca ccgcgtccgc gcatgccgga atcggagata gccttgatga cttccgaacg
     1081 ggtcatcttc aatgcggcct tcaggccttc gccagccttg atttgatcga aggtcagcgg
     1141 ttcgtgatac gacttaatca tgcgtggccc tccttgttct gcagggcgta aacgccaaag
     1201 gttctgcggc aggtctcgag aatctcgtga accttttccg gggtcaggcc gacataaagg
     1261 gtctcgttga ccagcatcgc cgggccctga tcgcatgctc caaggcaatt cgtccattca
     1321 agcgtgaact tgccgtccgg ggtggtttcg ccgaaatcga cacccagatc ggacacgagc
     1381 tgattcgcga tctgatcctt gcccgccatg tcgcaggaaa tggtgcggca aagccggacg
     1441 acgtacttgc cgtgcgtatc gtgactcagg aacgagtaga acgacacgac gctatagact
     1501 tcgaccggat ggatgtcgag ctcgtcggca acgacctgca tcgcgaattc cgagcactgg
     1561 tggtacttcc tgtgaatttc ctgaagtatc gggataaggt agcaacggtc tttgccgtaa
     1621 tgcttgaccc acgctgcaat atcctgcttc agaacgtcct gttcgctgac aagcattgcc
     1681 tcatttctcc ttatgcaaaa gctcggccac ggtttccagg agcttcttcg gcggaatcgg
     1741 cttttcctgg aacgcgtcaa ccgggaccag atccgagtcg gaaccgaaag acattccggt
     1801 caccctgctg atcgaagtca gcatcaggat gggcttcgca aagccaaccc gacgaagatc
     1861 ctgggccatg acaatcccgt catccggctg ctccatcatc acgtcgagga tcagcagatc
     1921 gggatttacc ttttgaatct gggccttccc gtccgtgacg ttgttggcgg acgcaacctc
     1981 gtaaccgttc ttttcgagga tcagcgtgca cgcatcaacc acatccgggt cgtcgtcaac
     2041 gatgagtatc cttgccacaa gagactccct tgcttcggcg cccatacaat ccgcgcccgc
     2101 cggtcccgcg ccaagaccaa ccggacacaa cccggttcca gaatcacccg ggttcagaac
     2161 accatcgtca gaagatccga acggatcaca accatggcgt ccgggcaatc ttttacctta
     2221 ttatgtaaat aaattaaagg tttttcgctt catgaaccct ttctggcgac aagggcaaaa
     2281 tatcggcatc cgcaccacgc aaggcattga attatcagac caatcccggc acggaatccc
     2341 ctgagcaagc ggtcacagcg atacagcgat gccaatgatg acagttatct tattcacaat
     2401 gacaaaaacc gtgctttcag atgcgtccgg ggactcgcga ggagcagaag tcgcgacacc
     2461 ggcaatcgac ttcaggcggc ataaacgccg atgacggtct tccagtgaaa ctcgtccggc
     2521 gcatcgcccg cccaggcttc gatgattccg gagaccggga gccgcccggc accgttgagc
     2581 aacgcaaaca cgcgttccct gatcgtgggc acggaagggt tgtcgatgtg gatgatcagc
     2641 agttttccga tcctggtcac ggcgacgctg tccatgtttt cccccggtgt tgtggtttga
     2701 cgccgggtat ttcgaacgtc acggaaaagc gtttcactgg cgtacgacgt gaatggcagg
     2761 gaggtgctgc cgcgaccatg cgaacagaca gggattcgac tggttccatg tgggccggcg
     2821 gggaatcagg actgatctga ccggtgctgg ctggagatgg ctggattggt gcgggcgtta
     2881 cttgttgatg cgcttttcgc caatcgcatc gaccgtgccc tggcccagga cggaaccatc
     2941 ccaggcataa accttgatgt aacggtcacc gccgccaatc gccctgtccc aggaaagatc
     3001 ggcgacgaag actccggaca taacatcgac ggtttccgcg gcaaccacct tgtcgttcgc
     3061 gatttcccag ttggcctgga tctcggcgtc gctttccgga tcctgaatca gttcaccggc
     3121 aaattcgttc cgactgactt ccagcgtaac gtaggcgcgg ccatcagcga agccggcaac
     3181 cgttccggaa acggtcaact gctttccggc aagggttccg ttcacatcca gagagcttac
     3241 cgatgcgggc ggatacttca tcatgaccgc gggatcgcca agcaggttgt aaagatccgc
     3301 atgctcgcga tagagccttt ccgggtcgtc cgcgccctgt tctccgtatg cgatggaagc
     3361 cccgtcgatg gtctgccgga agtcgtcgct gtgattgatc aattccgcct tggttcgcat
     3421 gaagagttcg cccgccgtct ttgggcgctc gccaagaccg acccgggtga cttcgtacgc
     3481 gagcaccgcg ttgccgacgg gatgtgattc gtcggacgag gcgaacacag ccaccggacc
     3541 gtcgggaagg tgcatcatgg cttcggcgat gctgtcgtcg ccccagtcga aattgccgtt
     3601 caggcaggcc aggaagacca tgaccggaag tcggttttca cacttgattt cgggaagctg
     3661 gtcggtctta agcccctggg tccagtcctc gttgccgtga ccgatgtaga tgctcatcaa
     3721 cgagccctgg ttgatcagct cgaccacctt ttcatggaaa ggagtgtagt agtagctgga
     3781 attttcgctg tcgtacgcgc cgatgatgtc gaaagcgtga ctgatcaggt caagaccctg
     3841 gaatgtgaaa tactcaagcg tggcgtcaat ctcgggtgaa aaaccggcct cgccgacgta
     3901 aagggaaacg cgacggttcc acaggccggg cgtgtatccg gcttcgtgat tcacaacctt
     3961 ctgaaggtaa tcgcggactt cctggttgtt cgacgcggtg atgcgaccaa gggcgacgtc
     4021 cgggatgtag tcgtcgtcaa ggtcggcaat cgagttgtcg gtgaagcaga tgccggtcag
     4081 gacattgtcg cagtcggttg tcggcagggg cttcgtgaaa ccttcctcgg gcacgtcctc
     4141 cacgtctccg acaagcacga cgaaaagagt ctcttcggcg ggcaactttt tgaagagctt
     4201 gtttatgtgg gcacgcgcca tgttcgtcca gttggccgtc gaaaccgtgg ttccacccag
     4261 aatgtcggat ctggtcatga cctgggttcg gaagcccctc gactggcggt actcggccag
     4321 ttccgatgcc gattcgagca gggaatccga ggcgatcaca aggtaattga cctcggtcga
     4381 taccacctgc acatctgaat cgaatgacga atcgggatcc acgacatcga ccggctcggt
     4441 cgagtcaccg ccgccatcgg actcgaccgc gtcctgaccc ggcagatcca ggcccgcgtc
     4501 ttccggaaca acgtcgtcgc cgggtgtatt gacatccagc cggggattat cggttcccag
     4561 ggcatccggg gcgccgtcaa ggtcgccttg aacggaatcg ccgccgcagc cggcaaccag
     4621 gaggatcgaa atcagcacaa cgggaatgag gtgaagcttc aaggaagact ccagaaagaa
     4681 accggcagat tatccgtgtt tgaaggccct gcgtcaatcc ggccggcaat ccggcagatg
     4741 cccggcgggc ttcgcgacgg ttcgacgccg gcgggctgga gatcggcccg gcggcgcgga
     4801 ttcaggacgt ttgaccggca aaccggccgc cttcacggcc gattcgggat cagagctttc
     4861 cgatcagcga gaacgaaatg accagggagt agatgaccag ggattcgatg agcgccaggc
     4921 ccagaatcat cgggacaaac atctttccct gggcgcctgg gttgcgggca atcccttcca
     4981 tcgcggcggt aacggccttt ccctgtgcca tggcgccacc gaaggcggcg atggcgatgc
     5041 cgaaaccggc ggccagcgcc acgaacacct ggacggtgaa cttgttgtcg cccgcggcga
     5101 cagcttcctg cgcgaaggcg gacggaacca gaacaagcat cgaaacgaac gtggcgccga
     5161 cggcggccag agccttcctg aacagggaca tcatctttcc tccacgataa agggccaagc
     5221 ccgtcaagaa ccgaaaaacg gccagcacgc cacgcggtca ggcgtggtgc gcgtcacctt
     5281 caccatgctc ctcggtggcc atgtagatgt acacggtcga gaggatgcag aataccagcg
     5341 tctggacaac ggcgacgaat ataccaagaa ccatgggaat caacggcatc acgaccgata
     5401 cgcgggcgat ccagggaccg ccggcaagcg tgcccgccgc catcagaagg aacgcggcaa
     5461 gcgcggcatg atcgccgaac atgtttccga aaagacggat tgcaagcgag accgggcggg
     5521 cgatgtggct gatcatttcg atgcagaaca tcagaagcat cagtggaagc gcgtaccact
     5581 tgatgatcgg cccgaagaac tccttgaaat acccgattcc gtgtgtcttg accccgtagt
     5641 agtgagtcgc gaagaagacc accagcccca gggccagcgt cgtgttcaga ttgctggttg
     5701 gcggcaggaa acctggaata agaccaagaa ggttagaaaa aagtatgaaa attccaaggg
     5761 atccgatcag tggaaggaaa tagagtgcct tttcgcggcc catcaggcct tccatcaatc
     5821 caagcagcgc gtcggaaagg aactggaaca gggccagagg ggaaaaacgg gcctcgggga
     5881 tcacggatcc gcgtccggag cgcatcttga cggcggcaag caccatcagg acgacaagaa
     5941 tggcaaacac cagcacgcca aaaacgacgt ggttcaggtc ggtgacaggc tccttgccga
     6001 taagggttcg cccgagcccg tgcgcaacgg cgtcggttac gtcggccggg attacttcgc
     6061 tggccagaga ccagtggtca tcgcttgccg atgccggaac ggaaccggac aacagcacga
     6121 ttgcgagtgc ggtcagtgat gacgcaaaac gaaacttcat ccgctttccc caggcacgac
     6181 ggccatcaca ggccggccat cacaaagtaa ccccgtcgcc gcgaagggca acgaaacagg
     6241 acatcgaaag tacggcgacc accgtcgtcg agaccccgat cgcgagcccg acagggctta
     6301 ccgacaggat tccgacggcc agaagcgcaa ggccggcaag aacccccatc ttgaccagca
     6361 gcaacgccac cgtcgatgcc cgcccagcgc cgggcgaaaa catccgcatg gcgatcaggc
     6421 cgatcaccag gacgttcagg gaaccggcca gcccgccggc aagcgcggca aaacctggcc
     6481 cctttccggc aaccgcgaga cttacggcac ccgcgaccgc ggcaagaacc gcattgacgc
     6541 aaacggtgtt cctgacgaaa cgcctggctg tcaaacgggc tcctcaaacg gaaccggggc
     6601 cttcatcgtc cccggcccag tacttcctgg ccgtacgcca gaggccgcga aaggcggccg
     6661 ccgatccgag tgcaaggaac aacagcagga accacggcgc ggtatcaagc ttcccgtcga
     6721 tccagttgcc gatgaaataa ccaaccgtga tcgcaatcgc catctcgatg ccgacggaca
     6781 tcacggcaac ggccgatccc agcgggcctt tcttacggtt tgaagccact ggacccccaa
     6841 ggttccggcg cccacggcgt aagatgataa ccaattcgaa ttcatgctga aaggtcaaaa
     6901 acgcattcga cggtcgaaga tgttaaaatt ccaacagagt cacaaaacca gagcggcgcc
     6961 gctgtgaaaa tcggaacact tccgccggac gcgccgacta ctgaagcctg acggcggccg
     7021 cctttcccgc ctgcagactg acgcgggtcg aaaaatcggg gcggccttcg acaacgacct
     7081 tcagcatgaa atcgaccggt gtcggacgat ccatgggaac cattatcggc tggttggtaa
     7141 cgccgcgcgg ccgggcctcg ttgttcagaa acaccttccc gttctgcggc ccgaccacca
     7201 gcagcctcgc ctcgcgatag tcgatggaaa cgcgcatcgg cgcctttggc ggactttcgc
     7261 gatcgataag gaatgacgtg tcgacatctc ggcactgatc gcaactcgga tgggacagca
     7321 caagccggtg acgtccgggc tccagcatca gcccctcgac acgcccggtt ccgacgcggc
     7381 ggccatcgac ctgaatctgg actgccggcg gataggcgtg aatcgaaatc gggaacgctt
     7441 ccggcttggg cgcggcctgg ggcgcggctt ggggctcggc ctgaggcgag acc
//
LOCUS       DAOPPA010000100         7487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_10966_length_7487_cov_3.668864, whole genome shotgun sequence.
ACCESSION   DAOPPA010000100 DAOPPA010000000
VERSION     DAOPPA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10966_length_7487_cov_3.668864"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..806)
                     /locus_tag="PKH54_04440"
     CDS             complement(42..806)
                     /locus_tag="PKH54_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC99170.1"
                     /translation="MVGSVSTGGIFDSHVHVGRWVLPDFRGHSTSLEQSAAVLASSGI
                     DGALVMPTDSGDNAALVSALASYSGPVRFAAAAWAVPDDSAIGGLLDAHDFRAVKIHP
                     SFCRVPVNHDSWSPAISAARQRGLPVVVHCGRWQEMAGYGLLLQAADAWPDVKFVMAH
                     MGGDSPSLVLSAAVGVASRGLRNVFMGTESIREYWIVAEAIGIVGADRVLFGSDHNLN
                     SPVSFLAVIDAIGLDEQARSMVLGGNARKLFGFDNG"
     gene            1012..1263
                     /locus_tag="PKH54_04445"
     CDS             1012..1263
                     /locus_tag="PKH54_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC99171.1"
                     /translation="MPTYEYRCVKCGNQFEKVQKMSDAPVAECPACGAGDAQRLISHS
                     SFVLKGSGWYCSDYSKKSSGTTPEPSGGCCGCESGKCSH"
     gene            1428..2093
                     /gene="rplC"
                     /locus_tag="PKH54_04450"
     CDS             1428..2093
                     /gene="rplC"
                     /locus_tag="PKH54_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOC99172.1"
                     /translation="MSIAILGKKIGMTQFFDEDGRRLPVTVIQAGPCQVIKVKTAEGR
                     DGYDAVVLGLGNKKEYKISKPVLGQYKKAGLTVWPTTVKEIRVTQDEALMYPEGGTVD
                     VSMFQAGEKVDVTGTSKGHGFAGVMKRHGFHGSLRSHGRAHECHRHGGSIGMCQFPGR
                     VIKGKKMAGHYGVDTHTMLNLKVVAVFPEQNLIMVRGPVAGATNGQVILRKATKVRKR
                     KAL"
     gene            2157..3038
                     /gene="dapF"
                     /locus_tag="PKH54_04455"
     CDS             2157..3038
                     /gene="dapF"
                     /locus_tag="PKH54_04455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOC99173.1"
                     /translation="MKFFKYHGTGNDFLVVDARDGHPAGLTPEAARLLCDRNFGVGGD
                     GILLVERGTASSWFMRVINSDGTQAEMCGNGIRCVAKFIAEHLWEGDAPNSIPVETPA
                     GIRLCRLTRDRSGVVSTVAVSMGAPKTERADIPMTGRGSPFNVAVTVAGRPFVGNAVN
                     MGNPHFVIFEDIHGADAGHWGSILGSNELFPSGANIEFAGRIDDNHFRLTVFERGCGL
                     TMACGTGASATAVAAVSNGLASSGRPIRMDLPGGPLFITVADDMSDVILEGPAMEVFS
                     GSVDIQALCRTRGADHE"
     gene            3031..3591
                     /gene="folE"
                     /locus_tag="PKH54_04460"
     CDS             3031..3591
                     /gene="folE"
                     /locus_tag="PKH54_04460"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOC99174.1"
                     /translation="MNEKDFEGFMNGFIAELGDDPSRPGLERTPARVWESMHYLTRGY
                     REDLNELMAGATFADPSRDLVMVRDIAFNSICEHHVLPFFGTVTVGYIPNGSLIGLSK
                     IARVVDHFACRLQVQERMTAQIADTLVDVLNPRALAVTVTAKHLCMAVRGVSKQDSNA
                     VTFAWRGQWSDDEAGRGEFLAGIGLR"
     gene            3610..4617
                     /locus_tag="PKH54_04465"
     CDS             3610..4617
                     /locus_tag="PKH54_04465"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOC99175.1"
                     /translation="MKPDPLSSPQAQETIRLIEAAATVVLVTHDGPDGDGIGSQIGLA
                     RALRRAGRKVWIVNAGSYPRSIRFLDSGNDVIVFNESHHQRNPHLFQEADLVILVDTG
                     EFERIGCLGERVQARQGPVVCIDHHQPGERAIPGIVAPDFTSCGELIVHVIDALGIEI
                     TKDVATALYGAILSDTQQFRFTKQDPDVFAVAARLVAAGAEPETIAGRMFGTMTRDRL
                     TLMTRIFNSARFECGGRLVHSVMTQEMISDLSVDREEIRNMVNLIGDLEGVEISALFK
                     VFKPGVVKVSMRCKQGLVISDVAEILGGGGHKQAAGADIEGDLAQIQASVVKMLINKL
                     N"
     gene            complement(4624..6054)
                     /gene="glgA"
                     /locus_tag="PKH54_04470"
     CDS             complement(4624..6054)
                     /gene="glgA"
                     /locus_tag="PKH54_04470"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOC99176.1"
                     /translation="MLNILNVTSEVIPFSKTGGLADVSFSLPSALSGLGHNVHTITPL
                     YSTVDRQACGVSFTEQVVDVVLAGRLIQFGVFQATTPAGVKVFLLENHDLFTREGVYG
                     TRDGAYGDNHLRFAFFCAAVFEVIKRFNLKVDVIHCHDWQSGLVPLFNRIRLQNQYRT
                     VMTVHNMGYQGTFPGVIAPEIGIPWDVYNPDGVEFWGQVSFLKAGLVYSDRVTTVSPR
                     YSREVLSPEMGYGMDGILRSLGNRFCGILNGVDYAQWNPETDELIAARFGIDDMSGKD
                     VNRQALLRHFGLADSGGPLFGVVGRLAYQKGYDLLAGIIPKIVELGGMLVVLGTGERA
                     VEDMIRTAAAGYPDRVSVKLEYSEAVAHTIEAGSDFFLMPSRYEPCGLNQMYSLRYGT
                     IPVVHAVGGLDDTVIDVDADPAGGTGIKFSPFEFRAFCDAIERAARLFGDDVRFGEVR
                     VRGMAKDFSWKNSAARYAELYQAVRK"
     gene            complement(6138..>7487)
                     /locus_tag="PKH54_04475"
     CDS             complement(6138..>7487)
                     /locus_tag="PKH54_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOC99177.1"
                     /translation="EDVLDTWFSSGLWPFSTLGWPDRTAALKTFYPTTVMETGFDIIF
                     FWVARMMMMGIHFMDDVPFKTIYLHAMVRDEHGLKMSKTKGNVIDPLEVVEEVGSDAL
                     RFTLANMTAQGRDIKLSMDRLKGYREFVNKLWNAARFIFMNLDDFTGTVPSDPAVVAA
                     NPKLGRADRWILNGLAQASADCNKALDEFRFNEAASVLYQFTWHQFCDWYLELAKGSL
                     MPDSDDVQTDPERRRAVQTVLIHVLDHVLRSMHPFMPYVTEEIWQKLRPFLSHDEDSV
                     MVANYPTGEGMPQFPEDAAAVESVLEVISAVRGVRAQAMVPPREELPVVIKPHSAEID
                     GFIRSMQEEVARLGRVASLDIRMDATRPAGSAVVVGTTADCFVVIGQDRIAAEIARLE
                     KEIARNGKDLEKFDAKLSNQAFIANASPEVVDEVREKADAARELMNRMNEARKALRD"
BASE COUNT         1594 a         2233 c         2209 g         1451 t
ORIGIN      
        1 taaacgccat gataacgatg gaaagatacc ccggactttc gtcagccatt atcaaatcca
       61 aacagtttcc gggcgttgcc gccaagaacc atcgaacggg cctgttcatc caggccgatt
      121 gcatcgatta cggccaggaa cgagaccggc gaattcagat tgtgatcgga accgaaaagc
      181 acccgatcgg ccccgacaat gccgattgcc tccgcaacaa tccagtattc ccggatgctt
      241 tccgtaccca tgaacacgtt acgaagcccg cgcgacgcca ccccgacggc agccgacagg
      301 acaagcgacg gagaatcccc tcccatgtgg gccatcacga atttgacgtc gggccaggcg
      361 tcagccgcct gcagaagcaa cccgtaaccg gccatttcct gccatcgccc gcaatgcacg
      421 acaaccggaa gtccgcgctg acgggccgct gaaatcgccg gcgaccagga atcgtgattc
      481 accggaacgc gacagaatga tggatgaatc ttgaccgcgc gaaagtcgtg cgcgtcaagc
      541 aggccgccaa tcgcggagtc gtccggaacc gcccaggcgg cggctgcgaa tcgaacgggc
      601 ccgctgtacg aagccagcgc actgaccagg gccgcgttat caccggaatc ggtcggcatt
      661 acaagggcac cgtcgattcc ggacgaagcc aggacggccg cggactgctc aaggctggtt
      721 gagtgccccc tgaaatccgg cagaacccat ctgcccacgt gaacgtgcga atcgaaaatc
      781 ccgccagttg aaacgcttcc aaccattaat aaaaccccaa taacaccttg cagggcagcg
      841 gtattgtaaa ccggccgcgc cgtccaggcc aatcctacct tgacaatggc aacccgcggc
      901 catagatatt gcctgcccgc ctggacaggt tccctgtttg tgaccgaccg ggggacgcgg
      961 gctgatgttc ggtgcccggc atttcgcctg gtaataagag atggtgcctt catgccgact
     1021 tatgaataca gatgcgtaaa atgcggaaat cagtttgaaa aggttcagaa gatgtcggat
     1081 gccccggtgg ccgagtgccc tgcgtgcggt gctggcgacg ctcagcgcct gatatcgcat
     1141 tcatcctttg ttcttaaggg atccggctgg tactgcagcg actattccaa aaagtcttcc
     1201 ggaacgactc ccgaaccgtc gggcggctgc tgcggatgcg aatccggcaa atgctcccac
     1261 tgacgacgcc tgttcccggg cggcgcgaca agagaaagac ttcattcccg tcaaagtttg
     1321 cgattgacac gaacctgccg ccagattact atattgcgcc gtttgagtat ggccttgacg
     1381 gcctttaccg cggtttgatt actgatattg atgattcgga gttgaatatg tcgatcgcaa
     1441 tccttggcaa gaagatcgga atgacgcagt ttttcgacga agacggaagg cgtctgccgg
     1501 taacggtcat tcaggctggt ccctgccagg tcatcaaggt caagaccgct gaaggtcgcg
     1561 atggttacga tgccgtggtt ctgggtctgg gcaacaagaa ggaatacaaa atctccaagc
     1621 ccgtgcttgg ccagtacaag aaggccgggc tgaccgtgtg gccgacaacg gtcaaggaaa
     1681 tccgcgttac ccaggacgaa gccctgatgt acccggaagg cggcaccgtt gacgtgtcga
     1741 tgttccaggc cggcgaaaag gttgacgtca ccggaaccag caagggccac ggatttgcgg
     1801 gcgtcatgaa gcgccacggt ttccacggct cgctgcgttc gcatggccgt gcccatgaat
     1861 gccaccgtca cggcggttcg atcggcatgt gccagttccc cggccgtgtc atcaagggca
     1921 agaagatggc cggtcattac ggcgttgaca cccacacgat gctgaacctg aaggtcgtcg
     1981 cggtgttccc ggaacagaac ctgatcatgg tcaggggccc cgttgccggc gccactaacg
     2041 gccaggtcat ccttcgcaag gccaccaagg ttcgcaagcg caaggccctg taatccggat
     2101 cacgtccccg attcatcctt atggacaaca ccggcacccg gtgtaatgat gtcgccatga
     2161 aatttttcaa atatcacggc accggaaacg actttctcgt ggttgacgcc agggacggcc
     2221 atcctgccgg ccttacgccg gaggcggccc gccttctgtg cgaccggaac ttcggcgtcg
     2281 gcggcgacgg aatactgctg gtcgaacgcg gaaccgcatc ttcatggttc atgcgggtca
     2341 tcaacagtga cggtacccag gcggaaatgt gcggcaacgg catccggtgc gtggcgaagt
     2401 tcatcgccga acacctgtgg gaaggcgatg ccccgaattc aatcccggtc gagacccctg
     2461 ccggcatcag gctttgcagg ctgacccgcg accgttcggg tgtcgtgtcc accgtcgcgg
     2521 tcagcatggg cgccccgaag accgaacgag ccgacattcc catgaccggc cgcggcagcc
     2581 ctttcaacgt cgctgttacc gtggccgggc gccctttcgt tggcaacgcc gtcaacatgg
     2641 gaaatccgca cttcgtcatt tttgaagaca tccacggcgc cgatgccgga cactggggat
     2701 caattctcgg ttctaatgaa ctttttccgt cgggtgccaa tatcgaattc gccggacgca
     2761 tcgacgacaa ccatttcagg cttaccgttt tcgaacgggg gtgtggcctg acgatggcct
     2821 gcggcaccgg ggcttcggca acggccgtcg ctgcggtgtc aaacggactg gcgtcatccg
     2881 gaaggccgat ccgcatggat cttccggggg gaccgctgtt cattaccgtt gccgacgaca
     2941 tgtccgacgt aatactggaa ggtcccgcca tggaagtgtt ttcgggatct gtcgatatcc
     3001 aggcgctttg ccgtacccgg ggagccgacc atgaatgaga aggatttcga aggattcatg
     3061 aacggcttca tcgccgaact tggtgacgat ccgtcacgcc ccggccttga acgaaccccc
     3121 gcccgtgtct gggaatccat gcattacctt acgcggggat atcgtgagga tctcaacgaa
     3181 ctgatggccg gcgcgacgtt cgccgatccc tccagggacc tggtcatggt cagggatatc
     3241 gctttcaact cgatctgcga gcatcacgtt ttgccatttt tcggcacggt gacggtgggc
     3301 tacatcccga acggcagtct catcggcctg tcgaaaattg cccgtgtcgt tgaccacttc
     3361 gcttgccggc tgcaggttca ggaaaggatg accgcccaga tcgcggacac gctggttgac
     3421 gtattgaacc cccgggcact tgccgtgacc gttacggcga agcacctgtg catggctgtc
     3481 cggggcgtct cgaaacagga ctcgaacgct gtcacgttcg catggcgcgg acaatggagc
     3541 gacgatgaag ccggccgggg cgaattcctt gctggaatcg gcctgcgctg accactggtg
     3601 aacatgctca tgaaacccga ccctctttca tcgccacagg cccaggaaac gattcgactg
     3661 attgaagcgg ctgccacggt cgtgctggtg acccatgacg gaccggacgg ggacggtatc
     3721 ggctcgcaga tcggacttgc gcgcgcgctt cgcagggctg gtcgaaaggt ctggatcgtc
     3781 aacgctggca gctacccaag atccatccgc ttcctggaca gcggcaacga cgtgatcgtt
     3841 ttcaacgaaa gccatcacca gagaaacccg catctttttc aggaagccga ccttgtgata
     3901 ctggtcgata ccggtgaatt cgaacggatt gggtgccttg gcgaaagggt acaggcaagg
     3961 caggggcccg tcgtctgcat cgatcatcac cagcccggcg agcgtgccat acccgggata
     4021 gtggcgcctg acttcacttc gtgcggcgaa ctgattgttc atgtaatcga cgcactcgga
     4081 attgaaatca cgaaagacgt cgcgacggcg ctttacggcg cgatcctttc cgacacccag
     4141 cagttcaggt tcacgaaaca ggatccggac gttttcgcag ttgctgcccg ccttgttgcc
     4201 gccggcgccg aaccggaaac catcgccggc aggatgttcg ggacgatgac ccgggatcgc
     4261 ctgacgctga tgaccaggat tttcaattcg gcacgctttg aatgcggtgg acgactggtc
     4321 cacagcgtca tgacccagga gatgatttcg gatctatcgg tcgatcgcga ggaaatccga
     4381 aacatggtca acctgatcgg cgacctggaa ggcgtcgaaa tatctgcgct tttcaaggtt
     4441 tttaagcccg gggtggtcaa ggtatcaatg cgctgcaagc agggactggt catatcggac
     4501 gtggcggaga tccttggcgg tggcggccac aagcaggcgg ctggtgccga tatcgaggga
     4561 gatctggccc agattcaggc aagcgtcgtc aagatgctga taaataaact gaattgaaag
     4621 cagtcatttc ctgacggcct ggtaaagttc ggcgtacctg gcggccgagt tcttccacga
     4681 aaagtccttc gccatcccac gaaccctgac ttccccgaaa cgaacgtcgt cgccaaacag
     4741 ccttgccgca cgctcgatcg catcgcagaa ggccctgaac tcgaatgggc tgaacttgat
     4801 cccggtgccg ccggccgggt cggcatccac atcgatcacc gtatcgtcaa ggccacccac
     4861 cgcatgcaca acagggatgg ttccgtaacg caaggaatac atctggttaa gtccgcacgg
     4921 ctcgtatctt gaaggcatca ggaaaaagtc ggagccggcc tcaatcgtgt gggcgacagc
     4981 ctcgctgtat tcgagcttga ccgaaacccg gtcgggatag ccggcagcgg ccgtccggat
     5041 catgtcctcg acggcgcgtt ccccggtacc gagaacgacc agcatgccac caagttccac
     5101 aatcttcgga atgattccgg ccagcaggtc gtatcccttc tgataggcaa gacggccgac
     5161 aacgccgaaa agcgggccgc cgctgtcagc aagcccgaaa tgccgcagaa gtgcctgacg
     5221 gttgacatcc ttgcctgaca tgtcgtcgat gccgaaccga gccgcaatca actcatcagt
     5281 ttccgggttc cactgggcgt aatcaacacc gttcaggatg ccgcagaaac gattacccag
     5341 actgcgcagg attccgtcca tgccgtagcc catctcggga ctcaggacct cacgggaata
     5401 gcgcgggctg acagtggtga cgcgatcgga atagacaagt ccagccttca ggaaactgac
     5461 ctgtccccag aactcgacgc cgtcggggtt ataaacgtcc cacggaatcc cgatttccgg
     5521 cgcgatgacg cccgggaaag tcccctggta tcccatgttg tgaacggtca tcaccgttcg
     5581 atactgattc tgcaaacgaa tgcggttgaa cagcggaacc aggccggact gccagtcatg
     5641 gcaatgaatg acgtcgacct tcaggttgaa gcgcttgatg acctcgaaca cggccgcgca
     5701 gaagaacgcg aagcgcagat ggttgtcgcc ataggcgccg tcacgcgtgc cataaacgcc
     5761 ctcgcgcgtg aaaaggtcat gattttcaag aaggaagacc ttgacgcccg ccggtgtcgt
     5821 ggcctggaac acgccgaact gaatcaggcg accggcaagg acgacatcga ccacctgttc
     5881 agtgaatgat acgccacagg cctggcgatc caccgtggaa tacagcggcg taatcgtatg
     5941 cacattgtga ccaagaccgg aaagcgccga cggcagcgaa aaggaaacgt ccgcaagtcc
     6001 gcctgttttc gagaacggga tgacttccga agtcacgttc aggatattca gcatttcacg
     6061 aatccgcgtc tgtctggacc tgacgggata ctagcaggga ttaagctgca aattcaacga
     6121 ttcttgtgtg gaatttgtca gtcgcgaagg gccttccggg cttcgttcat ccgattcatc
     6181 agttcacggg cggcatccgc cttttcgcgg acctcgtcca cgacctctgg actggcgttg
     6241 gcgatgaagg cctggttcga cagcttcgcg tcgaactttt ccagatcctt gccgttgcgg
     6301 gcgatttcct tttccaggcg cgcgatttcc gccgcgatcc tgtcctggcc gatgaccacg
     6361 aagcagtcgg ccgtggtgcc gacgacgacg gccgaaccgg ccggcctggt cgcgtccatc
     6421 ctgatatcca ggctggcgac acggccaagg cgggccacct cttcctgcat agatcggatg
     6481 aacccgtcaa tctcggcgga atgcggcttg atgacgacgg gcagttcctc cctgggcggc
     6541 accatcgcct gggcgcggac gccacggact gcgctgatga cttccaggac ggactcgacc
     6601 gcggcggcgt cttccgggaa ctgcggcatg ccttcgccgg tcgggtaatt cgcgaccatg
     6661 acactgtctt cgtcgtgcga caggaacggg cgcagcttct gccaaatctc ttcggtcacg
     6721 taaggcatga acgggtgcat cgaccgcagg acgtggtcca ggacatgaat cagcacggtc
     6781 tggaccgcgc ggcggcgttc cggatcggtc tggacatcgt cggaatcggg catcagcgag
     6841 cccttggcca gttcaaggta ccagtcgcag aactggtgcc aggtgaactg gtacaggacc
     6901 gacgcggcct cgttgaacct gaattcatcc agcgccttgt tgcagtccgc gctggcctgg
     6961 gcaagaccgt tcaggatcca gcggtcggcg cggcccagct tcgggttggc ggcgacgacc
     7021 gccgggtcgg acgggaccgt gcccgtgaag tcgtccaggt tcatgaatat gaatcgcgcg
     7081 gcgttccaaa gcttgttgac gaactcgcgg tatcccttca ggcgatccat cgacagcttg
     7141 atgtcgcgac cctgggccgt catgttagca agtgtaaagc gcagggcgtc ggagccgacc
     7201 tcttcgacga cttccagcgg atcgatgacg ttgcccttgg tcttgctcat cttcaggccg
     7261 tgctcgtcgc gcaccatggc gtgcaggtat atcgtcttga acggcacgtc gtccatgaag
     7321 tggatgccca tcatcatcat gcgggcgacc cagaagaaga tgatgtcgaa cccggtctcc
     7381 atgacggtgg tcgggtagaa ggtcttcagg gcggctgtcc ggtcgggcca gccgagagtc
     7441 gagaagggcc acaggccgct gctgaaccac gtgtccagca cgtcctc
//
LOCUS       DAOPPA010000101         7457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11013_length_7457_cov_3.417725, whole genome shotgun sequence.
ACCESSION   DAOPPA010000101 DAOPPA010000000
VERSION     DAOPPA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11013_length_7457_cov_3.417725"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKH54_04480"
     CDS             complement(<1..220)
                     /locus_tag="PKH54_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99178.1"
                     /translation="MTDDERFATLPVPPCLILVDKPVGITSTRVVSIVKRLMHVSRTG
                     HGGTLDPFASGLLPVMVGREFTRQADELL"
     gene            complement(213..632)
                     /gene="rbfA"
                     /locus_tag="PKH54_04485"
     CDS             complement(213..632)
                     /gene="rbfA"
                     /locus_tag="PKH54_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOC99179.1"
                     /translation="MQPYKRTDRASSVIREAISTILLNEVRDPDASKAIVTHVKVSAD
                     FSLARINVRCMQDDPEARKQMLRALRKAAGFLRTRLATQLNLFKTPELVFHYDDVPDK
                     GARIESLLKDLSGGRTMDQVAADEGFDRDNAFDGKDD"
     gene            complement(644..931)
                     /locus_tag="PKH54_04490"
     CDS             complement(644..931)
                     /locus_tag="PKH54_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOC99180.1"
                     /translation="MVVGVCRITVAIPESEWADTKRSVVKKIIGRTRASFNVCLAELV
                     DEDVPGTAVLAMAAVGSDHCVVNSVIDKAVTFIQELYLAEIEDYSVELIHL"
     gene            complement(947..3580)
                     /gene="infB"
                     /locus_tag="PKH54_04495"
     CDS             complement(947..3580)
                     /gene="infB"
                     /locus_tag="PKH54_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOC99181.1"
                     /translation="MGRRVYEVAKELGVESADVLKKLRDSGEILKDHRAPLTREQEDR
                     IRKLFAIQKEGEVQVKRMDGGRIVRRRAGAVAAPGQGEAPKPEDAAPAAPVPVSETAA
                     TEPVVAEVAAEQPVVAEGAVTGDVPEAKVEAAVVEPAVVDVVTPVSVEAPSQERAIDA
                     ASDVPPEAGVKTAAVRSGAPADARPETAAETAAREKEESQRKGKQKPRKDEDVPKASQ
                     VVDKDDAKAKGGKKLVFDRRRGVITLQDFSYGAEDESDHFEQRNQNRRKAPKPQRRGR
                     AMKTQVTMPSQVKRNIKVECDEITVAELAHRMSQKATELVRKLMGLGIMASVNQAIDF
                     ETASLLANDFGFTVEKTGFCLDEQLREIEDNPEDLRPRPPVITVMGHVDHGKTTLLDQ
                     IRNADVADHEAGGITQHIGAYKVKTDGGDIVFLDTPGHEAFTAMRARGASATDIVILM
                     VAADDGVMAQTKEAIAHARESGAPIIVAINKIDKPDANLDKTKQELAQEGLISEEWGG
                     DTIICEISAKKNIGVDRLKEMILLQSEVMELKANPNKPARGVVLESQLDRALGPVATV
                     LVQSGTLRVGDAVVSGMAHGRVRLMVDDKGRTLEEAGPATPVRVAGLDTVPDAGDVFA
                     VVADEKKAREIADFQIETNRKARAAVGASRVSLEDLFNMVQSGDVKDLKVIVKADVQG
                     SMAPLVDSVLKLKHPMITLKIIHQAVGNVAESDVNLAIAANAVIVGFNVNVDPKARSL
                     ADQEKVDIKRYSVIYDVIDDLKKAMEGLLAPKLVAKLIGKAEVRQVFAVSKVGKIAGS
                     MVFEGVVNRNCTVHVMREGKKVFEGKLFSLKRFKDDAREVKSGLECGIGVDGFEDIAQ
                     GDILEFYEFEEVRETISSPNA"
     gene            complement(3590..3862)
                     /locus_tag="PKH54_04500"
     CDS             complement(3590..3862)
                     /locus_tag="PKH54_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOC99182.1"
                     /translation="MTDVPFRPERKCVGCGKLRDRAELARVVIGADGAFELDTALARG
                     GRGAYVCRNPECLKRAVLRGHLARALRVPPGPDREAVLARLGEALK"
     gene            complement(3859..5316)
                     /gene="nusA"
                     /locus_tag="PKH54_04505"
     CDS             complement(3859..5316)
                     /gene="nusA"
                     /locus_tag="PKH54_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOC99183.1"
                     /translation="MSTETNWSEIIDLVSREKGIPRDVFIEALEEAILKAARKVFGEE
                     RSLEASFNPDVGQVELFQYMTVVDEVDSPLTQLSAAEAEMVDPEAQPGDELGFQIFYL
                     EADKDKAREEDMKHGDILKIQTYRNAFGRIAAQTAKHVVLQKVQEAERTQVYNEYRDR
                     LGDLVTGVARRFEKGNVIVDLGKAEAILPLREQCPRESYRAGDRVQAFVKDVTKEPRG
                     HQIILSRTDPRFVTKLFEKEVPEIYEGIVKIMAVAREAGERTKVAVASSDSDVDPVGA
                     CVGMKGSRVQAVVQELRGEKIDIIPWSVDLARYVCAAIAPAVVQRVLIDENNHQVELI
                     VADDQLSLAIGRRGQNVKLASKLLDWNIEIHGESRIQELKDRLKTELVKLEGIDESTA
                     EYLFKLGYHSPDNLLNADENELAAIPGIGREKAEAIQQTAFELKQRLKDESVSGREKA
                     GDADGSNGDAGASAGPESPVAGDAAASVGEAGDPV"
     gene            complement(5592..6926)
                     /gene="der"
                     /locus_tag="PKH54_04510"
     CDS             complement(5592..6926)
                     /gene="der"
                     /locus_tag="PKH54_04510"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOC99184.1"
                     /translation="MSTAKPIIAIIGRPNVGKSTLFNRILGRRAAIVDDFPGVTRDRQ
                     YGEAEHCGREFIVEDTGGFEPGSEDGMLVLMAAQVETAIAEADALVFVVDGRQGLLPI
                     DQVIWHQIRRSGMTVFVAVNKIDTPAADSLVSDFYGLGATELYPMTAEGGSGVAELLD
                     ALVETIPMPTVASDDGEASADDEMSGPCRITLLGRPNVGKSTLANALLGKERFLTSDV
                     PGTTRDAIDTVFHSNGREYVLVDTAGIRRRRAVERGVERMSVARTIRAIENCHVVVLL
                     IDSSEGVTDQDKKLASLVIDRGRGLVIVVNKWDLKTGGNAAGDFKLHMQDEMAFTSFA
                     PVVFTSALTGKNVGKLLPVVDHVHAGLFSRIGTSELNRFFSEVIERHPPASHGAKTVR
                     IKFITQVQVNPPTILLFCSGTGRIDTSYFRYLQRELYSRYDFEGVPIRLVQR"
     gene            complement(7032..>7457)
                     /locus_tag="PKH54_04515"
     CDS             complement(7032..>7457)
                     /locus_tag="PKH54_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99185.1"
                     /translation="GPFREVQIASLASMTHLHGLDRWGRAWLAEFNRLLGTRAAMAGR
                     DGDALKLLQRAVTVNPDDFRAWHNLGVLADAGGDSGIAVKLVANSVSINPGYARGWQT
                     LAAVAGRAGRHDLARDSLRRAAALLGTQPTRAAVSPAEK"
BASE COUNT         1443 a         2400 c         2072 g         1542 t
ORIGIN      
        1 gcaggagttc gtcggcctgg cgcgtgaact cgcgtccgac cattaccggg agcaggccgg
       61 aagcaaacgg gtcaagcgtg ccgccgtgac cggttcggct tacgtgcatc agccgcttga
      121 cgatggaaac aaccctggtg gatgtgatac cgacaggttt atcgaccagt atcaggcatg
      181 gaggaaccgg aagggtcgcg aatctttcgt cgtcagtcat cttttccgtc aaaggcattg
      241 tcccggtcaa atccttcgtc ggcggcgacc tgatccatgg ttctgccgcc ggaaaggtcc
      301 ttcagaaggg actcgatcct ggcgcccttg tcgggaacgt cgtcgtaatg aaaaaccagt
      361 tcgggggtct tgaacaggtt caactgggtc gccagccggg ttcgaaggaa ccccgcggcc
      421 ttgcgaaggg cacgaagcat ctgcttgcgc gcttccgggt catcctgcat gcacctgaca
      481 tttatgcgcg ccagcgagaa atcggccgat accttgacgt gcgtcactat tgccttcgac
      541 gcatccgggt cccgaacctc gttaagcagg atggtggaaa tggcctcgcg aatcacgctg
      601 cttgccctgt cggttctctt gtacggctgc atcgcgcacc tgactataaa tgaatcaact
      661 caaccgaata atcttcaatt tccgccagat acagttcctg gatgaatgtc acggccttgt
      721 cgataaccga attgaccacg cagtgatcgc ttccgacagc ggccatcgcc agcacggcgg
      781 ttcccgggac gtcctcatca accagttcgg caagacacac gttgaaagac gcgcgcgtcc
      841 ttccaataat cttcttcacg acgctgcgct tcgtgtcggc ccactccgat tcgggaatgg
      901 cgaccgtgat cctgcagact ccgacgacca tggcgcaccc cagtcatcag gcattcggcg
      961 aactgatcgt ctcgcggact tcctcgaact cgtagaactc aaggatgtcg ccctgggcga
     1021 tgtcctcgaa tccatcgaca ccgataccgc attcaagccc ggacttgacc tcgcgggcat
     1081 cgtccttgaa ccgcttcagg ctgaagagct ttccttcaaa caccttcttg ccctcgcgca
     1141 tcacatggac cgtgcagttg cggttgacga cgccttcgaa gaccatcgaa cctgcaatct
     1201 tgccgacctt ggacacagcg aagacctggc ggacttcggc ctttccaatc agcttggcca
     1261 ccagcttcgg ggcaagcaga ccttccatcg ccttcttcag gtcgtcgata acgtcgtata
     1321 tgaccgagta ccgcttgata tcgacctttt cctggtcggc caggctgcgg gccttcggat
     1381 cgacgttgac gttgaaaccc acgataacgg cgtttgccgc gattgccagg ttgacgtcgg
     1441 attcagccac gttgccgacc gcctgatgga taatcttcaa cgtgatcatc gggtgcttca
     1501 acttcagaac cgaatcgacc agcggggcca tcgatccctg gacgtctgcc ttgacgatga
     1561 ccttcagatc cttcacgtcg ccggactgaa ccatgttgaa caggtcttcg aggctgacac
     1621 gggaagcgcc gacggcggcc cttgccttgc ggttggtctc gatctggaaa tcggcgattt
     1681 cgcgcgcctt cttttcatcg gccacgacgg cgaacacatc gccggcatcc ggcacggtat
     1741 cgagaccggc caccctgacc ggagtcgcgg gaccggcttc ctcgagcgtt cttcccttgt
     1801 cgtcaaccat cagtcggacg cgaccgtgtg ccattccgga aaccaccgca tcgccgacgc
     1861 gcagcgtacc ggactgaacc agaaccgtcg caaccggtcc cagcgccctg tcgagctggg
     1921 attccaggac aacgccgcgg gccggtttgt tcgggttcgc cttcagttcc atcacttcgg
     1981 actgaagaag gatcatctcc ttcaacctgt cgacgccgat attcttcttg gcggaaatct
     2041 cgcagatgat cgtgtcgccg ccccactcct ccgaaatcaa gccttcctgg gccagttcct
     2101 gcttggtctt gtccagattt gcgtccggct tgtcgatctt gttgatcgcg acgattatcg
     2161 gagcgcccga ctcacgtgcg tgggcgatgg cttccttcgt ctgggccatg acgccgtcgt
     2221 cggccgcgac catcagaatg acgatgtcgg tcgcggaagc accgcgtgcc cgcatcgcgg
     2281 taaatgcttc gtggccgggg gtgtccagga agacgatgtc gcccccgtcg gtcttgacct
     2341 tgtaagcgcc gatatgctgg gtgatgccgc cggcctcgtg atcggcgacg tcggcattcc
     2401 gaatctggtc aagcaacgtg gtcttgccgt ggtcaacgtg acccatgaca gtaataaccg
     2461 gcggacgcgg acgcaggtcc tcggggttgt cctcgatttc gcgaagctgt tcatccaggc
     2521 agaacccggt cttctcgacc gtgaacccga aatcgttggc aagcagcgaa gccgtctcga
     2581 aatcaatggc ctggttgacc gaggccatga ttccaaggcc catcagcttg cggaccagtt
     2641 ccgtcgcctt ctggctcatg cgatgggcca gttcggccac cgtgatctcg tcgcactcga
     2701 ccttgatgtt acgcttgacc tggctgggca tagtgacctg cgtcttcatc gccctgccgc
     2761 ggcgctgagg cttcggagcc tttcgccgat tctggtttcg ctgctcgaaa tgatccgatt
     2821 cgtcctcggc gccgtacgaa aagtcctgaa gggtaataac gccacgacga cggtcgaaaa
     2881 ccagtttctt gccgcccttg gcctttgcat catccttgtc aacaacctgg ctggccttcg
     2941 ggacgtcttc gtccttgcgt ggcttctgct tgccctttct ctgcgactct tccttttcgc
     3001 gcgccgccgt ttcggccgcc gtttccggac gggcatccgc gggagcacca gatcgcacgg
     3061 cggcggtctt gacgccggcc tcgggcggta catccgacgc cgcatcgatc gcgcgctcct
     3121 gcgacggagc ctcgaccgaa acgggcgtaa ccacatctac aaccgccggt tcgacaacgg
     3181 ctgcctcgac ttttgcctcg ggcacatccc ccgtaacggc tccctcggcc acgacaggct
     3241 gttcggccgc gacctcggcg accaccggct cggttgccgc ggtctcggaa accggaacag
     3301 gagccgcggg ggcggcatct tccggcttgg gggcttcccc ctgaccaggc gcggcgacgg
     3361 caccggcacg acgacgaacg atgcgcccac cgtccatcct cttgacctga acctcaccct
     3421 ctttctggat cgcgaaaagc ttcctgatcc gatcctcctg ctccctggtc aagggggcac
     3481 gatgatcctt caggatctcg ccggaatccc gcagcttctt cagcacgtcg gcggattcga
     3541 cgcccagttc cttcgcgacc tcatagacgc gtctgcccat acggaaacct cacttcaaag
     3601 cttcccccag ccgggcaaga accgcttcgc gatccggccc cggaggaact ctcagcgcgc
     3661 gtgccagatg cccgcgcaga acggctcttt tcaaacattc ggggttgcgg cacacataag
     3721 cgcccctgcc acccctggca agcgccgtat cgagttcgaa cgctccgtcc gccccgatca
     3781 cgacccttgc aagctccgcc cggtcgcgaa gcttcccgca cccgacacac ttacgttcgg
     3841 ggcggaaagg aacatcggtc aaacgggatc ccccgcctcg ccgacggaag ctgccgcgtc
     3901 accggccaca ggggattccg gccccgccga tgccccggca tcaccgttcg aaccatcggc
     3961 atcgccggcc ttttcacgac cggagaccga ctcgtcctta aggcgctgct tcagttcgaa
     4021 cgccgtctgc tgaatcgcct cggccttctc gcgcccgatg cccgggatcg cggccagttc
     4081 attttcgtcg gcgttcaaca ggttatccgg cgaatggtag ccaagcttga acaggtattc
     4141 ggccgtcgat tcgtcaatgc cttccagctt gaccagttcg gtcttcagcc tgtccttcag
     4201 ttcctggatc ctcgattcac cgtgaatctc aatattccag tcaagcagct tgcttgccag
     4261 cttgacgttc tgaccgcgac gaccgattgc cagcgaaagc tggtcatcgg ccacgatcaa
     4321 ttcgacctgg tggttatttt catcaatcag gaccctctga acgacggccg gagcgatcgc
     4381 cgcgcacaca tacctggcca ggtcaaccga ccacggaatg atgtcgatct tttcgccccg
     4441 aagctcctgg acaaccgcct gaaccctgga gcccttcatg ccgacgcagg cgccgaccgg
     4501 atccacatcg gaatcggaag acgcgaccgc gaccttcgtc ctttcgccgg cttcccgggc
     4561 gaccgccatg atcttgacga ttccttcata aatttcggga acttcctttt caaacagctt
     4621 cgtcacgaag cgaggatcgg ttcgcgacag aataatctga tggccgcgcg gctccttcgt
     4681 cacatccttc acgaaggcct ggacgcgatc gccggcacgg tacgattccc tgggacactg
     4741 ttcgcgcaag ggaagaatgg cctcggcctt gccaagatcc acgatgacgt tgcccttttc
     4801 aaacctgcgc gcaacaccgg tgaccaggtc acccaggcga tccctgtatt cgttatagac
     4861 ctgggtccgc tcagcttcct gaaccttctg aaggacgacg tgcttggcgg tctgggcggc
     4921 gattcgacca aaggcgttgc ggtaggtctg aatcttcagg atgtcgccgt gcttcatgtc
     4981 ctcttcgcgg gccttgtcct tgtccgcttc aaggtaaaaa atctggaaac ccagttcgtc
     5041 gccgggctgg gcctcggggt caaccatctc cgcctcggcg gcagaaagct gcgtcagggg
     5101 gctgtcaacc tcatcgacga ccgtcatgta ctgaaacagt tccacctggc cgacgtccgg
     5161 gttaaaactt gcttccagcg aacgctcctc gccgaatacc ttgcgggcgg ccttcaggat
     5221 cgcctcttca agggcctcga tgaagacatc cctggggata cccttttccc ggcttacaag
     5281 atcaataatt tcggaccagt tggtttcggt cgacatcgtg tcctccgcac agaccatgtt
     5341 actggcaacg gcaccatcat gcggcatccg tcctggatga acgcataagt cattgaaaag
     5401 aactctcggc cacccccccc ccggcgaacg caattacccg caatcatgtt tgataattaa
     5461 ggcagtgtgg cgactggagg ggaaccagcc agaggaacgt acgcctttta ccgcagaccg
     5521 gaaaaaaata caagcataac aatgcttttt tgtcgaaaac gatcgcacgt cgggccaatc
     5581 gcgacttaag gctatctctg aaccagccgg attgggacgc cctcgaagtc gtacctggag
     5641 taaagttcgc gttgaagata ccggaaatag cttgtatcga tgcgtccggt gccgctgcag
     5701 aagagcagga tggtcggggg attcacctga acctgcgtga tgaacttgat tcgaacggtc
     5761 ttcgccccgt gactggccgg cgggtgacgc tcgatgactt ccgagaagaa gcggttcagt
     5821 tcggatgtcc caatcctgga gaaaaggccg gcatgtacgt gatcgacgac cggcagcaat
     5881 tttccgacat tcttgccggt aagcgccgac gtgaacacga ccggggcgaa actggtgaag
     5941 gccatttcgt cctgcatgtg aagcttgaaa tcgccggccg cgtttccgcc ggtcttcaga
     6001 tcccacttat tgacgacgat gacgaggccg cgcccccggt cgatgaccag cgaggccagt
     6061 ttcttgtcct gatcggtcac gccttccgag gaatctatca gcagcaccac tacatggcaa
     6121 ttctcgatgg cccggatcgt cctggcaacg ctcatgcgtt cgacaccccg ttccaccgcg
     6181 cgacgacgcc tgattcccgc ggtatcgacc aggacgtact cccggccgtt cgagtgaaag
     6241 acggtatcga tggcgtccct ggtggttccc gggacgtcgc ttgtaaggaa tctttccttc
     6301 ccgagaaggg cattggccaa cgttgacttg ccgacgttcg gcctgcccag aagggtgatc
     6361 ctgcatggac cggacatctc gtcgtcggcc gacgcctctc cgtcgtcgct tgcgacggtc
     6421 ggcatcggaa tcgtctcgac aagtgcgtca agcagttcag cgacgccaga accgccctct
     6481 gccgtcatcg gatacaactc cgtggccccg agcccataga agtcggacac cagcgaatcg
     6541 gccgccggcg tgtcaatctt gttgacagcc acgaagaccg tcatcccgga acgcctgatc
     6601 tgatgccaga tgacctggtc gattggaagc agtccctgtc ggccatcgac gacgaacacc
     6661 agcgcgtcgg cctcggcaat cgccgtctcg acctgagcgg ccatcagaac cagcattccg
     6721 tcctcggaac caggctcgaa tccgccggtg tcctcgacga tgaactcgcg tccgcaatgc
     6781 tcggcctcgc cgtactgacg atccctggtc accccgggaa agtcatcgac gattgcggcg
     6841 cgacgaccaa gaatcctgtt aaaaagcgtt gacttgccga cgttcggcct gccgatgatg
     6901 gcaatgatcg gttttgccgt gctcatgatg acctccttga aaatggtcgt atgacggcgg
     6961 cccggcatct ggcggccggg cgaaatcagg cgcggatttt acagaaaaat tcgattatta
     7021 ccacacatga atcatttttc ggcaggtgag acggcggcgc gtgtcggttg cgtgcccaga
     7081 agcgcggcgg cgcggcgaag cgaatcacgg gcaaggtcat gcctgccggc ccgacccgca
     7141 acagctgcaa gcgtctgcca gccacgcgcg tatcccggat tgatggaaac cgaatttgcg
     7201 acaagcttca cggcgatgcc gctgtcgcca ccggcgtcgg caagaacgcc gaggttgtgc
     7261 cacgccctga aatcatcggg attgacggtg acggcccttt gaagaagctt cagcgcgtcg
     7321 ccatcacggc cggccatcgc ggcacgcgtt ccaagcagcc ggttgaattc ggcaagccag
     7381 gcacgtcccc agcgatccag cccgtgaaga tgcgtcatcg acgccagcga cgcgatttga
     7441 acttcgcgga acgggcc
//
LOCUS       DAOPPA010000102         7446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11036_length_7446_cov_3.692599, whole genome shotgun sequence.
ACCESSION   DAOPPA010000102 DAOPPA010000000
VERSION     DAOPPA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11036_length_7446_cov_3.692599"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKH54_04520"
     CDS             <1..865
                     /locus_tag="PKH54_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99186.1"
                     /translation="GDLESLLRNQSETLNSSILAMREHQEVLKRTAQSDTSALQDMNR
                     LIDEAVQEAVSKANLQAEAVAEKARLKLENELQDALSRIEALKEAADETASQRKQIAK
                     LEKQLEDTRRREAERRADAESREAAVRADMDETLELFGRLETGYKENIEALEEERDSL
                     QERLSETEDTVQDLREQVSRFESMAREFPALQEAAAKNEVLSAENRKLQRTLDRMQDR
                     VEELQDIEKEAASLRAQLETAKSAERLVRAELDDIRKKFVQVKSLLGLNHQESGDPED
                     PGAKAAGEGRS"
     gene            865..1281
                     /locus_tag="PKH54_04525"
     CDS             865..1281
                     /locus_tag="PKH54_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99187.1"
                     /translation="MKRPASSSIPIILLAVASSILAATGCTPSIGDACESSTDCDSNH
                     VCDLSQTGGYCTISPCPRAGCPGGSVCVRFDRLSSWCMQSCSIFDYCRDDYRCVTDFI
                     DCDGNLVDPFCNQVAGEPAGQGDDGAGSGVRDANEG"
     gene            1296..1745
                     /gene="rsfS"
                     /locus_tag="PKH54_04530"
     CDS             1296..1745
                     /gene="rsfS"
                     /locus_tag="PKH54_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOC99188.1"
                     /translation="MDSNEFKPAARKEKATSGIIGNDDLVKAILDAAWSKNAFATKVY
                     DVTGVVDYTDMFVILSGRSDRHVMAIAEAVEGQLKEQGIRAAGVEGRKSGAWVLLDFG
                     NVIVHVFEKHKRSYYDLDRLWLEAKTIDVPEPEWVLDFARMESSQFD"
     gene            1700..2494
                     /locus_tag="PKH54_04535"
     CDS             1700..2494
                     /locus_tag="PKH54_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOC99189.1"
                     /translation="MGFGLRQDGIQPVRLKGRPAGTILFAIRHLLQCLADSFLPRHCL
                     SCGTAMSATSAIDLCPACTREALLCGPDVCSRCGAPSDSPDTCICHDPAFETVDMARS
                     AFAWTGPPRSMVLALKYSRKWELARPMATVMAAVWAASGGGLPDLVTSVPAIPRRFLA
                     RGYNQAALLASAFSAISDFPADNFVLIRQGPPQSTRGLGANERRRLAAGSFHVRNGRD
                     VRGRRILLVDDVLTTGATVSDCARALKSAGARRVDVLTFARSVHLP"
     gene            2579..3922
                     /locus_tag="PKH54_04540"
     CDS             2579..3922
                     /locus_tag="PKH54_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99190.1"
                     /translation="MSIPRPGRFILAAVLTALLVGGAFTRASASEYTSVMSAADRDDK
                     WDGVLGIRYDWLMRQSSITREYICDPTDPGCPATAGIARRAELDSVRDTHFMNIDMRI
                     GLYRDFEFFATFPFAVMDRTELDFASGVRRGNSTVYPSSGAHLFEVDNRSATRHGFGD
                     MIVGLKVAAMNQFRKKHHPTLVFAFSYTAPTGTIRKAGGKGVGEGLHKLNLDITASRR
                     IAFFEPWFGLFAQYQAPANDRGTLFRTYNAKDQTKIGPGPDIGLRLGGDFYVWNAPQE
                     NKEPERFATVDLGFFAKYRFQGREYTDLFDAFGTSTCAGDPACVSPTSSKNLLAYNLT
                     TDGQQHAIQFMDGITDVGSYGIFSTWVGVNVQPIKYVSMAFRFTYAYETGHFLTNADP
                     GMNLKKTATTTMLSNADGDKAFNPVFNSDVDLPGNRFFSEGAHMYGIMLMLTGRY"
     gene            complement(3906..4394)
                     /gene="coaD"
                     /locus_tag="PKH54_04545"
     CDS             complement(3906..4394)
                     /gene="coaD"
                     /locus_tag="PKH54_04545"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOC99191.1"
                     /translation="MVSVIYPGSFDPVTDGHVGLIRRAASVFGHVVVAVAGNFRKSEL
                     FTRDERVQMLRTVTAGIRNVEIDAFDGLLVDYMRARGSRIVIRGLRAVSDFEYEFQMA
                     HMNGRLDPGIETVFMVASPEETFISSSVVREVAMLGGNIEGMVHPEVARLLKERLVSS
                     GR"
     gene            complement(4394..4981)
                     /gene="rsmD"
                     /locus_tag="PKH54_04550"
     CDS             complement(4394..4981)
                     /gene="rsmD"
                     /locus_tag="PKH54_04550"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOC99192.1"
                     /translation="MRIVAGKYGGRKLAAPKGRGVRPTLEKVREAVFDSLGPRFEGAS
                     VLDLFAGSGAMGFEALSRGAARVVFVDSEQRSLDAVRQNAAALKVVERSISLMALTAS
                     AAIRSLASKKERFDVVFVDPPWESGIYEQTLLELGLSGIVDPDGIVVVEHARRYPVDA
                     VHGPLVMTRDRTYGDACVAYFRIASAANEPRQVED"
     gene            complement(5003..6409)
                     /locus_tag="PKH54_04555"
     CDS             complement(5003..6409)
                     /locus_tag="PKH54_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99193.1"
                     /translation="MGKVNRAKVITAAHKHLAKGNLRKAVKEYEKLLKEDPADIRTKL
                     KVADLLFRLGEKERAVRTFEDVAKFYSEQGFLLKAVAVYKQVQKMKPDDIGIHLALAE
                     LYRQIGLAGDAITQYRQAIALQKKAGLSSQRLETARKMVELDQDNARLRISLAEELSA
                     EGMVDESVAEFRSAYRILREADLIDEAVLVAERLLYHQPDDWEVSQFLARHFIERRDG
                     LKALSRLQVCFKVKPEDPEVLELLAQVFEFLGQPHKAATVLKSLGRIYEKAGLKSERD
                     TTYHNILRLVPGDKEAAVVLGIEKAAPREAVIGEEIVFENGASPSTASARLRQAPPVP
                     PPPVMREEDIPIETDDGEIPIDEDGEDTNPEMQAPEPIVPGSKPLDFETFPAGSGEIE
                     VGQETGEARTIVQNLPFLGGNLDLKGVPPEIQDDIQSLEFMIDAGLEEEAADLLKEIQ
                     ERAGDLPCLRKYKFLKKS"
     gene            complement(6419..6643)
                     /locus_tag="PKH54_04560"
     CDS             complement(6419..6643)
                     /locus_tag="PKH54_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99194.1"
                     /translation="MKPTPTLIRLHMAQGYLARARKMIDSYRRTGNREMARALECEWD
                     DAAAGTRRQASIELLQALLSRVKRNRSITG"
     gene            complement(6766..7221)
                     /locus_tag="PKH54_04565"
     CDS             complement(6766..7221)
                     /locus_tag="PKH54_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99195.1"
                     /translation="MKFTINFKVFGVPDGELLDGNDPDNPLVLEIEGDEQELTELERT
                     VSTMNPGETREIEVDADETVGEWDESRIERIPVSQFAEGTEFEEGMLYAFGDEGHGAD
                     EGADEGVDEGEGEVFFRVIGREGDVVVCDFNHPLAGRRLKFVVTLLSAE"
BASE COUNT         1555 a         2322 c         2117 g         1452 t
ORIGIN      
        1 cggggatctt gaatcccttc tgcgtaacca gtccgaaacc ttgaactcaa gcatcctagc
       61 gatgcgcgaa caccaggaag tcctcaagcg caccgcacag agcgacacgt ccgctctgca
      121 ggacatgaac cggcttatag acgaggcggt acaggaggcg gtttccaagg cgaacctgca
      181 agccgaggcg gtcgccgaaa aggcccgctt gaaactggaa aacgagctcc aggatgccct
      241 gtccaggatc gaggcgctga aggaagccgc ggatgagacg gccagccaga gaaaacagat
      301 agccaagctg gaaaaacagc ttgaggacac aaggcgccgc gaggccgagc gcagggccga
      361 cgcggaaagt cgggaagccg ccgtccgtgc cgacatggac gaaaccctgg agctttttgg
      421 aagacttgag accggttaca aggaaaatat cgaagccctg gaagaagaac gcgattccct
      481 tcaagagaga ctttccgaaa cggaagacac cgttcaggat ctgagggaac aggtttccag
      541 gtttgaatcg atggccaggg aattcccggc cctgcaggaa gccgctgcaa agaacgaggt
      601 tctttcggcg gaaaaccgga aacttcaaag gacgctcgac cgcatgcagg atcgggtgga
      661 agaactccag gacatcgaga aggaagccgc ctcgctgcgg gcccagcttg agaccgcgaa
      721 gagcgccgaa cgcctggtcc gggccgaact tgacgacatc aggaagaaat tcgtgcaggt
      781 caagagcctg ctgggattga accaccagga aagcggtgac ccggaagatc cgggcgcaaa
      841 agccgccggg gaaggccgtt cctaatgaaa agacccgcat cttcatcgat tccaataatt
      901 cttttggcgg tcgcttcatc aatcctggcg gcgacagggt gtaccccgtc catcggcgat
      961 gcctgcgaat ccagcaccga ttgcgacagc aaccacgtat gcgacctgtc tcagaccggc
     1021 ggttactgca ccatatcgcc gtgtcccagg gccgggtgcc cgggtggctc ggtctgcgtc
     1081 aggttcgacc gtctgtcgtc ctggtgcatg cagtcgtgca gcattttcga ttattgccgt
     1141 gacgattaca ggtgcgtgac cgacttcatc gactgcgacg gaaatcttgt tgacccgttc
     1201 tgcaaccagg tcgccgggga accggccgga cagggcgacg acggcgcggg ttccggggtg
     1261 cgggacgcga acgaagggtg aacaaccgga ggaaaatgga cagtaacgaa ttcaagccgg
     1321 cggcaaggaa ggaaaaggcg acatcgggga ttatcggaaa cgacgatctg gtgaaggcca
     1381 tccttgacgc cgcgtggtca aagaacgcct tcgctacgaa ggtttacgac gtcaccggtg
     1441 tcgttgacta caccgacatg ttcgtgatcc tttccgggcg gtcggatcgc catgtgatgg
     1501 ccatcgcgga ggcggtcgaa ggtcagttga aggaacaggg aatccgggcg gccggcgtcg
     1561 aggggcgcaa gtccggagca tgggtgctgc tggatttcgg aaacgtgata gtgcatgtgt
     1621 tcgaaaaaca caaacgcagc tactacgacc ttgatcgcct gtggctggaa gcaaaaacta
     1681 ttgacgttcc ggagccggaa tgggttttgg acttcgccag gatggaatcc agccagttcg
     1741 actgaagggc cgccccgccg ggacgattct gttcgcgatc cgacatctgc ttcaatgcct
     1801 ggccgattcc tttctcccgc ggcactgctt atcctgcggc accgccatgt ccgcgacctc
     1861 ggcaatcgat ctctgtcccg catgcacccg ggaagcgctt ctgtgcggcc ccgacgtctg
     1921 ctcaagatgc ggtgcgcctt cagactcgcc ggatacgtgc atctgccacg accctgcttt
     1981 tgaaaccgtg gacatggcca ggtccgcctt cgcctggacc ggcccgccca gatccatggt
     2041 tctggccctc aaatactcac gcaaatggga actggcccgg ccgatggcga cagtgatggc
     2101 cgcagtgtgg gccgcatccg gaggcggtct tcccgacctt gtaacaagcg tgccggcaat
     2161 tccgcgacgc ttcctggcca ggggatacaa tcaggccgcg cttcttgcct cggccttttc
     2221 cgcgatttca gactttccgg ccgacaattt cgtcctgatc cgccagggcc ccccgcagtc
     2281 aaccaggggt cttggcgcga acgagcgccg cagacttgcc gccggaagct tccacgtcag
     2341 gaatggccgt gacgtaaggg gcaggagaat ccttctagtc gatgatgttt tgacgacggg
     2401 ggccaccgtt tcggactgtg cacgagcgtt gaaatccgcc ggcgcccgcc gcgtcgacgt
     2461 gctgactttt gcaagaagcg ttcatctgcc ctgaaactcg cctgaacgat gctggtcggc
     2521 tttgacagta agaggctttc ttcagataat cggggggctg gttttcccgg gggttccgat
     2581 gtcgattcct cgtcccggtc gattcatcct ggcggccgtg ctgacggcct tgctggttgg
     2641 gggagccttc acccgggcct ccgcctcgga atacacatcg gtcatgtcgg ccgcggacag
     2701 ggacgacaag tgggacggtg ttctcgggat ccgttacgac tggttgatgc gccaatccag
     2761 catcacccgc gaatacatct gcgatccgac cgatcccgga tgcccggcga ccgcaggaat
     2821 tgcaagaagg gctgaactcg actcggttcg ggacacccat ttcatgaaca tcgacatgcg
     2881 catcggcctt tacagggact ttgagttctt cgcgaccttc ccgttcgccg tgatggacag
     2941 gacggaactc gattttgcaa gcggggttag gcgcggaaat tcaacggttt acccgtccag
     3001 cggggcccac cttttcgagg ttgacaaccg cagcgccacc agacacggtt ttggcgacat
     3061 gattgtcgga ctgaaagttg ccgccatgaa ccagttccgg aagaagcatc accccacgct
     3121 ggtgttcgcc ttttcgtaca cggcaccgac cggcaccata cgcaaggctg gcggaaaggg
     3181 tgtcggggaa gggctccaca agctgaacct cgatatcacg gccagccgga ggattgcgtt
     3241 tttcgagcca tggttcggcc tgttcgccca gtaccaggca cccgcgaacg accggggaac
     3301 tctgtttcgc acctacaacg ccaaggatca gacgaagatt ggaccggggc cggacatcgg
     3361 ccttcgcctt ggcggcgatt tctatgtctg gaacgccccg caggaaaaca aggagccgga
     3421 aagatttgcg acggtcgatc tcggcttctt cgcgaagtac cgcttccagg ggcgtgagta
     3481 taccgacctc ttcgacgcgt tcgggacatc gacatgcgcc ggcgacccgg cctgcgtcag
     3541 cccgacatcc tcgaagaacc tgctggccta taacctgacc accgacggac aacagcacgc
     3601 gatccagttc atggacggca tcacagacgt cggctcgtat ggaatctttt cgacctgggt
     3661 cggcgtcaac gtgcagccca tcaaatacgt gtcgatggct ttcaggttca cttacgcata
     3721 cgaaaccggt cacttcctta cgaatgccga tcccggcatg aacctgaaaa agaccgcgac
     3781 gacaacgatg ctttcaaatg cggacggcga caaggccttc aatccggtat tcaactcgga
     3841 cgtcgatctt cccggaaaca ggttcttttc cgaaggtgca cacatgtacg gaataatgct
     3901 gatgctcacc ggccgctact gacaagtctt tccttcagaa gcctggcgac ttccggatga
     3961 accatccctt caatgttgcc gccaagcatg gcgacttccc tgacgaccga ggaactgatg
     4021 aaagtctctt ccgggctggc gaccatgaat acggtctcga ttccgggatc aagcctgcca
     4081 ttcatgtgcg ccatctggaa ctcgtactcg aaatccgaaa ccgcccgaag tccccgaatg
     4141 acaatccgtg aaccgcgcgc cctcatgtaa tcgaccagaa gcccgtcaaa ggcatctatc
     4201 tcgacattcc ggatgccggc ggtcaccgtt ctcaacatct gaacccgctc atcacgggta
     4261 aagagttcag acttccgaaa gtttcccgcg acggcaacga ccacgtgacc gaatacggac
     4321 gccgcccgcc ttataaggcc gacatgaccg tcggtaaccg gatcgaacga accgggataa
     4381 atgaccgaca ccatcaatcc tccacctgcc gcggctcgtt cgcggcggac gcgatcctga
     4441 aatacgcgac gcaggcgtcc ccgtaagtac gatcccttgt cattacaagc gggccgtgca
     4501 cggcgtcaac cggataccgc ctggcatgtt caaccaccac gatgccgtcg ggatcaacaa
     4561 tccccgaaag gccgagttca agcagggtct gctcgtaaat tcccgattcc cagggaggat
     4621 cgacgaacac gacgtcaaaa cgttcctttt tcgatgcaag cgacctgatt gcggccgacg
     4681 ccgtaagtgc catcagactg atcgatcgtt ccaccacctt caatgcggcc gcgttctgcc
     4741 gaacagcgtc cagcgacctt tgctccgaat cgacgaacac gaccctggcg gcaccccggg
     4801 acagcgcctc gaatcccatg gcaccggaac cggcaaacag gtcaagcacg gaagctccct
     4861 cgaagcgcgg accgagcgaa tcgaaaaccg cctcgcgcac cttttccagg gtcgggcgga
     4921 cacccctgcc cttcggcgcg gccagcttgc ggccaccata ttttccggcg acgattctca
     4981 tgcgggcaga cctggtaagg tattaagact ttttaaggaa cttgtacttg cgaagacacg
     5041 gcaggtcgcc tgcacgctcc tgaatctcct tcagcaggtc ggcggcctcc tcctcgagtc
     5101 cggcgtcaat catgaactcc aggctctgga tgtcgtcctg gatctccgga ggcacccctt
     5161 tcagatccag gtttccgcca aggaacggca gattctgcac gatcgtgcgt gcctcgccgg
     5221 tttcctgtcc gacctcgatc tcgccgctgc cggccgggaa ggtctcgaaa tcaagcggct
     5281 ttgaacccgg cacgatcggc tcgggggcct gcatttcggg attggtatct tcgccatctt
     5341 cgtcgatcgg aatttcgccg tcgtccgtct ctatcgggat gtcctcctcg cgcatgaccg
     5401 ggggaggcgg aacgggcggc gcctggcgca gccttgctga cgcggtggaa ggcgaagcgc
     5461 cgttctcaaa gacgatctcc tcgccgatga cggcttcacg cggggcggcc ttctcgatgc
     5521 caagcaccac cgcggcttcc ttgtcgccag gaaccagccg gaggatgttg tggtaagtcg
     5581 tgtcgcgctc gctcttcagg ccggccttct cgtaaatacg gccaagggac ttgaggacgg
     5641 tcgcggcctt gtgcggctgt ccaaggaact cgaacacctg ggccagaagt tccaggactt
     5701 cgggatcctc cggtttcacc ttgaaacaga cctgcaacct ggacagcgcc ttcagtccgt
     5761 cccgccgttc gatgaaatgt cttgccagga actggctgac ctcccagtcg tcaggctggt
     5821 ggtaaagaag cctttcggcg accagtacgg cctcgtcaat cagatccgcc tcgcgcagaa
     5881 tcctgtaagc cgaccggaac tccgcgacag actcatcgac catgccctcg gcggaaagtt
     5941 cctcggccag ggaaatgcga agcctggcgt tgtcctgatc cagttcgacc atctttctgg
     6001 cagtttcaag acgctgcgac gaaagaccgg ccttcttctg aagggcaatc gcctggcggt
     6061 actgcgtgat ggcgtcaccg gccagcccaa tctggcgata aagttcggca agcgcaaggt
     6121 gaatcccgat gtcgtcgggc ttcatcttct gcacctgttt gtaaacggcg accgccttca
     6181 gcaggaagcc ctgttcggag tagaacttcg ccacgtcctc gaaagtcctg acggcacgct
     6241 ccttttcccc caggcggaaa aggagatccg cgaccttcag cttggtccgg atgtccgcgg
     6301 ggtcttcctt cagaagcttc tcgtactcct tgaccgcctt gcgcaggttg ccctttgcca
     6361 ggtgcttgtg cgccgccgtt atgacctttg cccgattgac tttgcccaaa tcacgacctc
     6421 atccggtgat cgacctgttt cttttcaccc gcgaaagcag cgcctgcaga agctcgatcg
     6481 acgcctgcct tctggttccc gccgcggcat cgtcccattc gcattccagc gcgcgggcca
     6541 tttcccggtt ccccgtgcga cgatacgaat cgatcatctt tctcgcgcgc gcaagatatc
     6601 cctgggccat atgcagcctt atcagggtag gagtcggttt caaggtccag cccccgcaac
     6661 ggtacggcga caggccgccc ggaatctttt cgccgacaac gacttaaaag tcaaggtgct
     6721 tcaagccgcc cggcacggcc atgcgacact ggaagcactt gaaattcact cggccgacag
     6781 gagggtaacc acaaacttga gcctgcgccc cgccagcgga tggttgaagt cgcagacgac
     6841 gacgtcccct tcccgcccaa tcacgcggaa gaagacctcg ccttcaccct cgtccacgcc
     6901 ctcgtccgcg ccctcgtccg cgccgtggcc ctcgtccccg aacgcgtaca gcatgccttc
     6961 ctcgaactcg gttccttccg cgaactgact caccggaatg cgctcgatcc tcgactcgtc
     7021 ccactctccg acggtctcgt cggcatcgac ctcgatctcc ctggtttccc cgggattcat
     7081 cgtgctgacg gttctttcca gttcggtaag ttcctgttca tccccttcaa tttcaaggac
     7141 aagggggtta tcgggatcgt tgccgtccag cagttccccg tcgggaacgc caaatacctt
     7201 gaaattgatc gtgaacttca tgggatcctc catgggcggg tccgcccggc atgaaaccaa
     7261 aagctgcgtc aactttgtca caacatgctt accggatcca catcaatcgt aacccgaaga
     7321 tcaccggcac cggtccggaa ccgacccctg accgcccaca gcagcctgtt catgacgctt
     7381 cgagaaggtc ccttcagcag gatgtgcagc cttgtgcggc cgcgaatccg ctcgatcgcc
     7441 gcaagg
//
LOCUS       DAOPPA010000103         7434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11052_length_7434_cov_4.510638, whole genome shotgun sequence.
ACCESSION   DAOPPA010000103 DAOPPA010000000
VERSION     DAOPPA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11052_length_7434_cov_4.510638"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="PKH54_04570"
     CDS             <1..136
                     /locus_tag="PKH54_04570"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOC99196.1"
                     /translation="FFRLAAQIRSGSIDESWLGSLEWRDSIFPVIDYTVYRTGSWKRP
                     "
     gene            173..700
                     /locus_tag="PKH54_04575"
     CDS             173..700
                     /locus_tag="PKH54_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOC99197.1"
                     /translation="MSTASIVDSRAGSRSMAAMSGILAVTGFIVLTAMGAHARFPLPF
                     TPVPVTMQVFAVILAGLCLTPRQAFASQAIYVIGGLSGLPLLAHAGLGTIGYLVGFVA
                     AAPLVSFAARRGLAAVGGLAGVLVIHVLGCVGLAASGVPAGLPVIAAGSLVFLPGDIL
                     KVLLAVKASRMARKN"
     gene            728..1756
                     /locus_tag="PKH54_04580"
     CDS             728..1756
                     /locus_tag="PKH54_04580"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOC99198.1"
                     /translation="MSDSMSVAVVGATGVVGTEAVALLEERGFPVGSLRLMASGRSAG
                     ETIGFRGKDVTIEALAGADFAGIDLIFSAPGASVSLEYVPRAVAAGALVIDKSSAFRM
                     APDVPLVVPEVNGDAASRETRIIASPNCSTIPLVMVLDPLHRRFGIRRVVVSTYQAVS
                     GAGQKAGDELIGQTVAILNRRPMPIEVFPHQIAFNCLPQVETFEPGLEGYSTEEQKVC
                     DETRKIMGIPDLRITATCVRVPVVNGHSESVNIEFESPVDPAEARKLLDSMPGIRIVD
                     SPAQGEYPMAVEASGLDEVLVGRIRRDFSVDNGLNLWLACDNIRKGAALNAIQIAERV
                     FGIDPVRS"
     gene            1925..3136
                     /locus_tag="PKH54_04585"
     CDS             1925..3136
                     /locus_tag="PKH54_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC99199.1"
                     /translation="MMNQKTTTWFLLAFSLLALVVPAPAVAAISAFKFLGIEGIGSSC
                     AMNSRLGIASGSTEHVINIQDCVDYTACDIEVRWGLDRTPATGTTYAIKVSLPGGACV
                     DNDFTTLGGTCMEEILVDEKTLSSPNYMTFSVPFDVLTGGDCQAGTSKSTKIYIIVKE
                     PGLDPTAEVLMFDVDLSRPPAPEIQEPVEGDSNITVKWTAIDESDDSTTQYNVYWSEA
                     TFSNSSKSSATVVGPMSGTSYQIKDLENDKEYFFAVSSIDENDNESVISAVTSGMPID
                     VSDFWEMYRSSGGQDDGGFCFLATAAWGSYMAPQVMTLRAFRDSVLLTSAPGRAFVSF
                     YYKVSPPLAGLISDSPLLRSVSRLALMPAVILARIALATSLWFAVFSGVALVALTVLF
                     FGVAIRTRRRP"
     gene            3133..3885
                     /locus_tag="PKH54_04590"
     CDS             3133..3885
                     /locus_tag="PKH54_04590"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOC99200.1"
                     /translation="MNIRIFSAAVVLVIVGGFVPSPASAEEEPKKHESPRRFFLEAKF
                     SPYRPAIDSEFGNATPFKDVFGGGQFLMTQVEFEYEIWNKVGIIGIGAVLGYSRLVGT
                     GINPVTLEEATDKTAMNVMPLAIHMLYKFDYLAQRYKVPLVPHVKAGFDYWIWWIENG
                     TGDVASVPGSSEGSVRKGYGGTWGGHVSLGIAFLLDFIAPSMAQTFDVDVGVNNSYLF
                     FEYTWAWINDFGVGNSMNLSAGMFVGGIAFEF"
     gene            3917..4696
                     /locus_tag="PKH54_04595"
     CDS             3917..4696
                     /locus_tag="PKH54_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC99201.1"
                     /translation="MLSSSGLSIGVVIPAFNPCHRLGDVLTGLPPFVSCVVVVDDGSN
                     PPISTDRLVSPRPVDVVRHESNRGVGAAIVTGYRRCIEMGIDVAVVMGADDQMDPADM
                     ERLVAPILAGRVAYAKGDRLSHPACRAVMPLPRLLGNSCLTFLTRLVTGLPVMDSQCG
                     YTAFRLDASGRLPLSHIYPRYGFPNDMLAALSGAGLPVCDVVVRPVYPGGRSGIVAPV
                     AAIVYPFVLARSILTRCLAAMSRGHRAFTLDSGSEAGKCAF"
     gene            4684..5865
                     /locus_tag="PKH54_04600"
     CDS             4684..5865
                     /locus_tag="PKH54_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC99202.1"
                     /translation="MRVLMLTTSWPLCDGDWRGGFVMDLCRDLAASGIDVDVAVPAPA
                     ASCIPEGFDSALPIAGVTVHWLPRLIQFRSRAFHQSGLEANLRKRPWSALSMGPVLGA
                     FAAECSLLAMRADVIVSNWLLPMGAVGATVAGVTGRPHLVIAHSAPAGPAAIPPVSTV
                     IRKVIESADSVACVSESVLESTAAIAGPELARKLKVIRLGIDLVPVVLHRPSSRPFEI
                     AFSGRIVPIKGADILVRAAAGIPGCRLVIVGDGPGLQYLRLLSARLGVNADFVGELSR
                     QDARKRIAMADVVVIPSRKGLLGRQEGLPRVLVEAWAAGVPVVASRSGGMTEVMDGQG
                     GGILFDPGDVEGLRRILAGLASDPHRLAELRAQAITRAAEFDAGVAAAAWAQWIRGAC
                     S"
     gene            5935..>7434
                     /locus_tag="PKH54_04605"
     CDS             5935..>7434
                     /locus_tag="PKH54_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99203.1"
                     /translation="MNEAGSNRRLVLAAMCALHLSVSGCFGDLIPDRPDLAGYDVSFP
                     DSFPFDLPPELPGDATGDGPACPRNCYERVCGPDGCGGTCGTCPAGTVCSLDYSICLV
                     TDVLRPAGSVCGQNAACEPKLENPVYPGTYYDNPDWPACTGYQCQGLVCDQGLCSAAC
                     EISVDQLVNGTDVSASDGIEDDVGTSDCPEFTGSGFKGPSICVSRGMANLGEPAGVCV
                     PWSSMAPCNPGSECGAGEYCGYIRIGSAVERRCLARITGGAATGSACGYDRPVDTNTL
                     CESGNCFSDWCTSPCDTDADCILPGNLCQAGLCAGTGRSCSIDSDCSPLSCGTVQVDR
                     IAVRGCVARSCTGDRDCPGDDLFCSPEVASVSAGPSAHQGRCRPVTEGGEPVGSPCGP
                     DLSGGQRVCRNTGMCLDGTCASTCDDDLDCFVQGSLRCSYRDRVYASGYLGIPVWLRS
                     GLCIPAGYATDGCRVDADCDGSSCTPWAGGEQAAPEVVTTCMKPAGESWP"
BASE COUNT         1235 a         2212 c         2334 g         1653 t
ORIGIN      
        1 ctttttcagg cttgccgcgc agatacgttc gggcagcatt gacgaatcgt ggctcgggag
       61 tcttgaatgg cgtgattcaa tcttcccagt cattgactac acggtgtatc gtaccggctc
      121 gtggaagcgt ccctgacgac ttcccgaatt ccggtcttga tggagtatcg aagtgagtac
      181 cgcatctatc gtggattcaa gggcaggctc gcggtcgatg gccgccatgt ccggcatcct
      241 tgccgtcacc ggtttcattg tgttgaccgc aatgggcgca cacgcgcgat ttccgcttcc
      301 attcaccccc gttccggtca cgatgcaggt gttcgccgtg attctggccg gcctctgcct
      361 gacgcccagg caggcgtttg cttcccaggc catctacgta ataggcggcc tttccggcct
      421 tccgctgctt gcgcacgcag ggctcggcac aatcggctat cttgtcggct tcgtggccgc
      481 ggccccgctc gtctcgttcg cggcacgccg gggcttggcg gcggtcggcg gtcttgcagg
      541 cgtcctggtc attcacgtcc ttggttgcgt gggtcttgcg gcatccggtg ttcctgccgg
      601 tcttcctgtc atcgcggccg gttccctggt attcctgccc ggcgacatcc tcaaggtact
      661 gcttgccgtc aaggcgtcgc gcatggcacg aaaaaactga atttttcaat agttttcggg
      721 ggtattgatg tcagacagca tgtcggtcgc ggtggtgggt gccaccggag tggttggcac
      781 cgaggcagtc gcgcttcttg aggagagggg atttcccgtt ggcagtcttc gcctgatggc
      841 ttccggccgc tcagccggcg agaccatcgg cttccgcgga aaggatgtca ccatcgaagc
      901 cctggccggt gccgattttg ccgggattga cctcatcttt tcggctccgg gggcctcggt
      961 cagcctcgaa tacgttccgc gggcggtcgc tgccggcgcc ctggtgatcg acaagtccag
     1021 cgccttccgg atggcgcccg acgtgccgct ggtcgttccc gaggtgaatg gcgacgcggc
     1081 atcccgggaa accagaataa tcgccagccc gaactgcagc accattccac tggtcatggt
     1141 tcttgacccg cttcatcgcc ggttcggaat ccgccgggtc gtggtcagca cctaccaggc
     1201 tgtcagcggt gccggccaga aggccgggga cgaactgatc ggacagacgg tcgccatcct
     1261 caacaggcgt cccatgccga ttgaagtctt tccccaccag attgccttca attgcctgcc
     1321 gcaggtcgag acttttgaac cgggccttga gggctattcg accgaagagc agaaggtctg
     1381 cgacgagacc cgcaagatca tgggaattcc cgatctgcgg ataaccgcga cctgcgtccg
     1441 ggtgccggtg gtcaacggtc actccgaatc ggtcaacatc gaattcgaga gtccggttga
     1501 tcctgccgag gcgcgcaagc tgctcgattc gatgcccggg atccgtattg tcgatagccc
     1561 cgcccagggt gaatatccga tggcggttga ggcatcgggt cttgacgaag tcctggtcgg
     1621 acgaatccga cgcgactttt ccgtcgataa cggtcttaac ctgtggcttg cgtgcgacaa
     1681 cattcgcaag ggggccgcgc tgaatgcgat ccagattgca gagcgcgttt tcggaatcga
     1741 cccggtgcgt tcgtgacaac gacgaccgga ttcgcccgcc ctgtttgaca aaatgacact
     1801 cttcggcgag cggggctgtc gcgtccgggc gttcccgatt gaaaaaacgg gtagttttcc
     1861 ggtagttttg ggcggactga aacagatttg ccggtggcac gatagttgct ttaatgtatc
     1921 ccttatgatg aaccaaaaaa caacaacatg gttccttctg gcgttttcgt tgctggccct
     1981 ggtcgtgccc gcaccggctg tcgcggcgat ttcggcgttc aagttcctgg gaatcgaagg
     2041 catcggttcc tcgtgcgcga tgaacagccg gctcggcatc gcttccggtt ccaccgagca
     2101 cgtgataaac atccaggact gcgtcgatta cacggcatgc gacatcgagg tcaggtgggg
     2161 actggacagg actccggcca ccgggacgac atatgcgatc aaggtaagcc ttccgggcgg
     2221 ggcgtgcgtc gataacgatt tcaccacgct tggcggcaca tgcatggaag aaatccttgt
     2281 tgatgagaag acgctgtcat ccccgaacta catgaccttc agcgttccgt tcgacgtcct
     2341 taccggtggc gactgccagg ctggaacaag caaatcgacc aagatatata tcatcgtcaa
     2401 ggaacccggc ctggatccga cggccgaggt tctgatgttc gacgttgatc tttcgcgccc
     2461 gcccgctccg gaaatccagg agccggtgga gggcgactcg aacatcacgg tgaagtggac
     2521 cgccattgac gaaagcgacg attcgacaac gcagtacaac gtctattggt ccgaggccac
     2581 gttctcgaat tcaagcaagt cgtcggcaac cgtcgttggt ccgatgtccg gaacaagcta
     2641 ccagatcaag gatcttgaaa acgacaagga gtatttcttc gccgtttcgt caatcgatga
     2701 aaacgacaat gaaagcgtca tctcggccgt aaccagtgga atgccgatcg atgtcagcga
     2761 cttctgggag atgtaccgtt caagcggcgg ccaggacgat ggcgggttct gcttcctggc
     2821 gacggcggca tggggcagct acatggcgcc ccaggtgatg acactcaggg ccttccgcga
     2881 cagcgtcctt ctgacgtccg ctcccggtcg tgcattcgtg tcgttctact acaaggtcag
     2941 cccgccactt gccgggctga tttcggacag cccgcttctc cgttccgttt cccgcctggc
     3001 gctgatgccg gcggtgattc tggcccggat cgcgcttgcc acgtcgctct ggttcgccgt
     3061 tttttccggc gtggcccttg tcgccctgac ggttcttttc ttcggcgtcg ccattcgcac
     3121 aaggaggcgc ccatgaacat caggattttc agcgcggcgg ttgtactggt cattgtcggc
     3181 ggattcgtcc cgtcgcccgc ctcggcggag gaagagccca aaaagcatga gtcgccaagg
     3241 cgctttttcc tcgaggcgaa gttttcgccg taccggccgg caatcgacag tgaattcggc
     3301 aatgccaccc cgttcaagga cgtcttcggc ggtgggcagt tcctgatgac ccaggtcgaa
     3361 ttcgagtacg agatctggaa caaggtcggc atcatcggca tcggcgccgt gcttggatac
     3421 tcaaggcttg tcggaaccgg aatcaatccg gtgacgctcg aggaggcgac cgacaagacc
     3481 gccatgaacg tcatgccgct tgcgattcac atgttgtaca agttcgacta cctggcgcaa
     3541 cgatacaagg ttccgcttgt tccccacgtg aaggccggtt tcgattactg gatctggtgg
     3601 atcgagaacg ggacgggcga cgttgcttcc gtgcccggaa gttcggaagg ttcggttcgg
     3661 aagggatacg gcgggacatg gggtggccac gtttcgctgg gaattgcctt cctcctggat
     3721 ttcatcgcgc cgtcgatggc ccagacgttc gacgtcgatg tcggcgtcaa caattcgtac
     3781 ctcttctttg aatacacctg ggcatggatc aatgatttcg gtgtcgggaa ctccatgaac
     3841 ctttctgctg gcatgttcgt cggtggcatc gccttcgaat tctgatgtct tttcgacact
     3901 tccgtcgggg tggttgatgc tgtcgtcgtc aggactttcc atcggcgtcg tcattccggc
     3961 cttcaatcca tgccatcggc tcggcgatgt gttgacgggc cttccgcctt tcgtttcctg
     4021 cgtggtcgtc gtcgatgacg gatcgaatcc ccccatttcg acggatcgct tggtctcgcc
     4081 gcggccggtc gatgtcgtcc ggcacgaatc gaacaggggc gtgggcgcgg ccatcgtcac
     4141 gggataccgg cgttgcatcg agatgggaat cgacgtggcc gtcgtcatgg gggccgatga
     4201 ccagatggat cccgcggaca tggagcgcct tgtggcgccg atccttgccg gcagggttgc
     4261 gtatgcgaag ggcgacaggc tttcccatcc cgcatgccgc gcggtgatgc ccctgccacg
     4321 acttctggga aattcatgcc tgacctttct caccaggctc gtcaccggtc ttccggtcat
     4381 ggattcgcag tgcggttaca ccgctttcag gctggatgcg tcgggaaggc tgccgttgag
     4441 ccacatctat ccaaggtacg gcttcccgaa tgatatgctt gcggcgcttt ccggggcggg
     4501 cctgccggtt tgcgacgtgg tcgtacgtcc ggtgtatccg ggtggccgtt ccggaatcgt
     4561 tgccccggtt gcggccattg tctatccttt cgtgcttgcc agatccatcc ttaccaggtg
     4621 cttggcggca atgtcgcggg ggcatcgcgc attcacgctg gatagcggtt cggaggccgg
     4681 aaaatgcgcg ttctgatgct taccacatcc tggccgctat gcgacgggga ctggcggggc
     4741 ggattcgtca tggatctgtg ccgcgacctg gccgcttccg gcatcgatgt cgatgttgcc
     4801 gtgccagccc cagcggcgtc ctgcatccct gaaggatttg attccgctct gccgattgcg
     4861 ggcgtcacgg ttcactggct tccgcggctt atccagttcc gctccagggc attccatcag
     4921 tccgggctcg aggccaacct gaggaagcga ccctggtcgg ccctgtcgat ggggccggtg
     4981 cttggggcat ttgcggccga gtgctccctt cttgccatgc gggccgacgt catcgtttca
     5041 aactggctgc ttccaatggg cgcggtcggc gccacggtgg ccggcgtgac cggcaggccc
     5101 cacctggtga ttgcgcattc ggccccggcc gggccggcag caattccgcc cgtctcgacc
     5161 gttatcagaa aagtaattga gtccgcagat tcagtggcgt gcgtgtcgga atcagttctt
     5221 gaatcgactg ccgcgattgc agggccggag cttgcccgga agcttaaggt catccgtctt
     5281 gggatcgacc tggtgccggt tgtgcttcac aggccttcgt cgcgcccttt tgaaatcgca
     5341 ttttccgggc gcatcgttcc aataaagggg gcggacatcc tcgttcgggc tgcggccggg
     5401 attcccggct gccgactggt tatagtcggg gacggcccgg ggcttcaata ccttcgtctg
     5461 ctttcggcaa ggctgggtgt caatgccgat tttgtcgggg aactgtcgcg ccaggacgcc
     5521 aggaagcgca ttgccatggc cgatgttgtc gtcatccctt caaggaaggg ccttctcggg
     5581 cgtcaggaag gcctgccccg tgtcctggtc gaggcgtggg cggcaggcgt gccggtggtc
     5641 gcatcgcgct cgggcggcat gaccgaggtc atggacgggc agggcggcgg catcctcttc
     5701 gaccccgggg acgtcgaagg gcttcgaagg atacttgccg ggcttgcatc ggatccgcac
     5761 aggcttgccg aacttcgggc ccaggcgata acgcgcgcgg ccgaattcga cgccggcgtc
     5821 gcggccgccg cctgggcgca atggatccgt ggcgcatgtt cttgaacggg cctgcccaaa
     5881 ctgatacctt tgtctgcgtc gttggtcggg ccgagtgttt cctggacgga cacgatgaat
     5941 gaagccggtt caaatcgtcg tctggtgctt gccgcgatgt gcgcgctgca cctttcggta
     6001 tccggctgct tcggcgacct gattccggac aggcccgatc tggcgggcta tgacgtcagt
     6061 tttccggatt ccttcccctt cgaccttccc ccggaacttc cgggcgatgc caccggtgac
     6121 ggcccggctt gtccccgcaa ctgctatgaa cgtgtctgcg ggcccgacgg gtgcgggggc
     6181 acatgtggaa cctgtccggc cggaacggta tgttccctgg attactctat atgccttgtg
     6241 accgatgtcc ttcgtccggc cggttcagtc tgcgggcaga atgccgcatg tgagccaaaa
     6301 ctcgagaatc cggtatatcc gggaacatac tacgacaacc ccgactggcc ggcctgtacc
     6361 ggttaccaat gtcagggatt ggtgtgcgat cagggactct gttcggcagc gtgcgaaatt
     6421 tccgtcgatc agttggtcaa cggaaccgac gtgtccgcca gcgacggtat cgaggatgac
     6481 gtcggcactt ccgattgtcc ggagtttacc ggaagtggat tcaaagggcc ttcaatctgc
     6541 gtttcgcgtg gcatggcaaa cctgggagag cccgccggcg tctgtgttcc atggtcgtcc
     6601 atggcgccct gcaacccagg cagtgaatgt ggggccggcg agtactgcgg gtacatcagg
     6661 atcggttccg cggtcgagcg gcgatgcctt gccaggatta cgggcggcgc tgccacaggt
     6721 tccgcctgcg gatacgacag gccggtggac accaatacgc tatgtgaatc tggaaattgc
     6781 ttttccgact ggtgtacaag cccgtgcgac acggatgccg actgtatcct gcccggaaat
     6841 ctttgccagg ccgggctttg tgctggtacc ggccgttcct gctcgatcga ttcagactgc
     6901 agcccgcttt cgtgcggcac cgttcaggtt gaccggattg ccgtgcgcgg gtgcgttgcc
     6961 agaagctgca ccggggatcg cgactgtccc ggcgatgacc ttttctgcag ccccgaggtc
     7021 gcctcggttt cggccgggcc ttcggcgcac cagggcaggt gccgcccggt tacggaaggc
     7081 ggcgagcctg tcggcagtcc ttgcggcccg gatctttcgg gcggccagcg cgtgtgtcga
     7141 aataccggca tgtgcctcga tggcacctgc gcctcaacct gcgacgacga tctggactgc
     7201 tttgtccaag gttcccttcg ttgttcgtac cgcgatcgag tgtacgcttc aggctatctt
     7261 ggaattccag tctggctccg atccggattg tgcatcccgg ccggatacgc caccgacggc
     7321 tgccgcgttg acgcggattg cgacggatcg agctgcaccc cgtgggccgg cggcgaacag
     7381 gccgcgccgg aagtcgtgac gacctgcatg aagccagcgg gcgaatcgtg gccg
//
LOCUS       DAOPPA010000104         7384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11147_length_7384_cov_3.729022, whole genome shotgun sequence.
ACCESSION   DAOPPA010000104 DAOPPA010000000
VERSION     DAOPPA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11147_length_7384_cov_3.729022"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1121)
                     /locus_tag="PKH54_04610"
     CDS             complement(63..1121)
                     /locus_tag="PKH54_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC99204.1"
                     /translation="MSKGRRIMFKVAVGIPVAALLLTGCALFWASLGWTDADLGRAEV
                     YDLDRSTPEATHSIRVMTWNIGFGGGLKGGPTDRHPGAEVRQNLGSIASAIKSARSDV
                     VLLQEVDRPSSRTGGIDQLEFLMKQTGLTHACFVATWKNNYVPFPYLPISGHIGPVVS
                     GQAVLSRFPIVECRRVGLPQPQSNKWWYNRFYLHRAVQHVILKVGSVASEDGTIDVMN
                     VHLEAFSNDNRRQQAAILVDYVRGHAASRPLVLGGDFNSIPPFSVKTKGFVDEDIDFS
                     GDDTIEQIQRIPGLSEVIIDETPGLPPTVFFTYPAQSPSRRLDYVFHRGFTGSILTRV
                     PRSATFSDHLPVITELLF"
     gene            complement(1121..2560)
                     /locus_tag="PKH54_04615"
     CDS             complement(1121..2560)
                     /locus_tag="PKH54_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC99205.1"
                     /translation="MTEDTEVTDGLSVAVFSTLVVDDEVLYARAIARALSRNSIRCEL
                     AHSASEAMEKVRTSIYDVILLDHKLPDGDGIELIPPILVAQKTAVVVVMTAFEAIPNA
                     IQAIRNGAEDYKVKQTSTQPLVDTVLEIMRRKKVYRGSGVPQTGLTGSSAQIRQVVDE
                     IAKVAPASSTVLLTGETGSGKEVAAREIHRRSGRGGKPFVAVDCASIPGSLIETILFG
                     HERGAFTGADQARNGAFFEAGSGTVFLDEIGEMPPDLQARLLRVIESRTFKRVGSVRE
                     LPMDARIVAATNRDLNQEVAAGRFRFDLFQRLSVFPIRLPPLRERGEDILILAGQFLA
                     QVCMAMGAPEARLSREAEAFLVSYDFPGNVRELKNVIERAVILTDTGIIEPRHLPERM
                     LGMNIGGSASTIRATAGASLPVEIAGAVNVGFVPGIDTLEGIQEKLILAVLEKAGGNR
                     SEAARMLGISRFALLRRLEKIVSRRQTGD"
     gene            complement(2577..4520)
                     /locus_tag="PKH54_04620"
     CDS             complement(2577..4520)
                     /locus_tag="PKH54_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99206.1"
                     /translation="MAGEPTGGILDVLTSILRGAAKLLGCTSANMVLMDEKAQTATVV
                     VATMAAIRNQIGEVENLVGAVQNVAFPLKAVSTSALAYRSWKERTAMETPSLAEIIGP
                     ELMPDEGVEAAMDLLGDHRFFLVPVISGSLSFGVLIFEKPGINPFSPQQRELLIRYAQ
                     RVGEILDMHMRASTFPQGADFGVIRENTALIRFLMDAGGRIVGYNSGVTVGADGGFLF
                     VLRDLVPAMEFSPGFVDQVRRQVVSFLKSDDEGIVPFRFVPEDNVRLKDTGFDIRIEV
                     SVSRIHTDAGDLALASFHEIRAHQGMATHQLVRLALGEASPVVLVGPDFCITSENEAA
                     DRLFGFDDGGMSGLSIAEIFRDAEDIGDLLDKQIMDLTSGYYEECALLRRKDGECFPG
                     YVEALLLVDADENQLGYLVRIREQWAPLDKAGGEHLVRRERLATMGELGAQVAHEIRN
                     PILAIGATLEAMSRDATDQDDVATFAGLGSEINRLDMLLRDYLSMAARHNAAMAPVDV
                     AGIIDDIRRLLCGSPRWGQRRLVSKVCPGEAVLADADSIRHVLFNLVLNAMEATSADG
                     TITVSSDRTQRDMAIYVDDDGPGLTASPREVFEPFFTTKANGTGLGLTVCRRIVQSLG
                     GAVSLRNREEGGCRASVVLPIPS"
     gene            4652..6160
                     /locus_tag="PKH54_04625"
     CDS             4652..6160
                     /locus_tag="PKH54_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99207.1"
                     /translation="MKKSSFLFLSAIITVAATIQGCGGATASGDTIVDNSPAELPDQD
                     AQDVHASDPGAVPDVSDVFDENLPEDGLSETHSDAVTDIIIPDPISTIGAFRFDNIEA
                     TSGQKDLFTTLLLNERHVTAVNRCGLMGIVFNDVVADGLGGFVSSLVFSEVNLSSPPG
                     EDELVSRVTLRSFSASSSPARHMATLAYESDCTPIVIVQTEDGFQFTQVGGGQITFNE
                     LDDDTDYQDIESLGARVGLDKKIHMMFRARPGAGATMQFFDATFNGSGFDVVAMPNPD
                     TALVSTIDAAAGNDGRAVVCYLKDGTDGQEVWVARFNGTTWDRERVAGVVPSQLRWIA
                     FTIGSYGRESIAWANVATNDEGLILSVDLKHTYRDLDKTFKTEAVAFENNGYQGGGGK
                     RFTGALPRILVDGSGRPHILFSDIAIETSGTSQTILTGQLRYAWRRNMKWNETTIFEQ
                     TPDQDGKDAFIEFRDFGTSPDGTAAGIAALEVVASDSTFQLSTIGISVVNVK"
     gene            complement(6168..7088)
                     /locus_tag="PKH54_04630"
     CDS             complement(6168..7088)
                     /locus_tag="PKH54_04630"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC99208.1"
                     /translation="MQSSEFYFNDSHGLPVHVHKWLPDGDVKAVIQVSHGMQEPAYRY
                     QAFARDMTAAGYAVYANDHRGHGKTLVDNVRGRLGEPGWQAVLSAMTQLTATIRAELP
                     GVPVFLMGHSWGSFLAQAYIQSFGDRVDGCVLSGTNGSNPLVAIGLVLARMVCAIRGG
                     ETKAQLLWQLSLGPYNKGLHDTDTGLDWLSRDTSVVRAHFEDPECGADFPNSFYLQML
                     MLLKHIWQPAHEKRIPCTLPIYIFAGSADPVGGYGKGVKALHDRYRKYGINDLQMRLY
                     EGGRHEMLNETNRVEVVQDLKTWLDSHVKA"
     gene            complement(7146..>7384)
                     /locus_tag="PKH54_04635"
     CDS             complement(7146..>7384)
                     /locus_tag="PKH54_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99209.1"
                     /translation="DMNVFAMSAEERAAKIKPLPSSLPEALDALESDHAFLEEGGVFS
                     KSLISDWISIHRKAEVEEMRSRPHPWEIETHLDC"
BASE COUNT         1676 a         2250 c         1955 g         1503 t
ORIGIN      
        1 atcctactgt acgtattccg ctcgatgtgg ttcaggaccg ttcccgaatc gacaaagccc
       61 gactagaaca gaagctcggt aattaccggc aggtgatcag aaaacgtggc gcttcgaggc
      121 accctggtaa gaatcgaccc cgtgaatcca cgatggaata cataatcaag acgccttgat
      181 ggactttgcg ccggataggt gaaaaacacc gttggcggca ggccgggcgt ttcatcgatt
      241 attacttcac taaggccggg gatgcgctgg atctgctcaa tcgtgtcgtc accggaaaaa
      301 tctatatcct cgtccacaaa acctttcgtc tttacggaaa agggcggtat ggaattgaaa
      361 tccccgccca ggacgagcgg cctcgaggcc gcatgccccc tgacataatc aacgagtatg
      421 gccgcctgct gcctgcggtt gtcattcgaa aacgcttcaa gatgaacatt catcacatcg
      481 attgtaccgt cttccgaggc gacagaaccc accttcagga ttacatgttg aacggcccgg
      541 tgcaggtaga accggttgta ccaccactta ttgctctggg gttgtggaag cccgacgcgg
      601 cggcattcca cgatgggaaa ccggcttagc acagcctggc cggacacgac cgggccgatg
      661 tgccccgaaa tcggaagata tggaaatggc acgtaattgt tcttccatgt agcgacaaag
      721 cacgcatggg tcaaaccggt ctgcttcatc agaaattcaa gctgatctat cccgcccgta
      781 cgcgaagaag gtctgtcaac ttcctgcaga aggaccacgt ccgatcttgc cgacttgatg
      841 gccgatgcga tcgaaccaag attctgacgc acctcggcac cgggatgcct gtcagtcggc
      901 ccgcccttca atccgccacc gaacccgata ttccaggtca tgacacgaat gctgtgggtc
      961 gcttccgggg tgctgcgatc aaggtcatag acctcggccc ggccaagatc ggcatcggtc
     1021 cacccaaggg atgcccagaa caatgcacat ccggtcaaca gaagtgccgc aaccgggatc
     1081 ccgaccgcca ccttgaacat gatccgtcgt ccttttgaca tcagtcacct gtctggcgcc
     1141 gggaaactat cttttccagg cgacgaagaa gggcaaatcg gcttattcca agcatgcggg
     1201 ccgcttccga cctgttgcca ccggcctttt ccaggacggc aaggatcagt ttttcctgga
     1261 tcccctcgag tgtgtcgatg cccgggacaa agccgacatt gaccgcaccg gcaatttcta
     1321 ccggcaggct ggccccggct gtcgcccgga tcgttgacgc cgatcccccg atgttcatgc
     1381 cgagcatcct ctcgggcaag tgtctgggct cgattatccc ggtatcggtg agaatgaccg
     1441 cacgctcaat gacgttcttc aactcgcgca cgtttccggg aaaatcgtac gaaaccagaa
     1501 acgcttccgc ctcgcgggaa agcctggctt caggcgcgcc catcgccatg catacctgcg
     1561 caagaaactg gccggcaaga ataaggatat cctcgccgcg ctcgcgaaga gggggaagcc
     1621 ttatcggaaa gaccgacaac ctctggaaca agtcgaacct gaacctgccg gccgcgacct
     1681 cctggttcaa atccctgttg gttgccgcga caatccttgc atccatcggc agttccctga
     1741 ccgacccgac acgcttgaaa gtccgcgact cgattacccg caggagtctg gcctgaagat
     1801 ccggcggcat ttcgccaatt tcgtcaagga acaccgttcc ggatccagcc tcgaaaaagg
     1861 caccgttcct tgcctgatcc gctccagtaa aggcgcctcg ctcgtgcccg aacagtatgg
     1921 tttcaatcaa tgatcccggg atcgacgcgc aatccacggc gacgaaaggc ttcccgcccc
     1981 gtcccgaccg ccgatggatt tccctggcgg caacctcctt gccggaaccg gtctcgccgg
     2041 taagcagaac cgtcgatgaa gccggcgcaa ccttggcgat ttcatccacg acctgccgaa
     2101 tctgggccga gctgccggtc aatccggtct ggggcacgcc gcttccccga taaaccttct
     2161 tgcgacgcat gatttccagt acggtatcga caagtggctg tgtactggtc tgcttgactt
     2221 tgtaatcctc ggcgccgttc cttatcgcct ggatcgcgtt cggtatcgcc tcgaacgcag
     2281 tcatgaccac gaccaccgcg gtcttctggg caacaaggat gggtggaatc agttcgattc
     2341 cgtcaccgtc cggaagtttg tggtcaagaa gaatcacgtc atatatggac gtcctgacct
     2401 tttccatggc ctcggacgcg gaatgggcaa gttcacacct gattgaattc ctggatagcg
     2461 cccttgcgat ggcccttgcg taaaggacct cgtcatcgac cacaagtgtg gaaaagaccg
     2521 caacggacaa accatccgtg acttccgtgt cttcagtcat tcagataccc tcgatatcac
     2581 gatggaatcg gcagaacgac agacgccctg catccgcctt cctcgcggtt gcgcagcgac
     2641 accgcaccgc caagtgactg aactatgcgc ctgcacaccg taagccccag gccggttccg
     2701 ttggccttgg tcgtgaagaa cggttcgaat acttccctcg gactggcggt caggccgggc
     2761 ccgtcatcat caacatagat ggccatgtcc cgctgggtac ggtcgcttga aacggttatc
     2821 gtgccatccg ccgacgtcgc ttccatggcg ttcagcacca gattgaacag aacatggcga
     2881 atcgagtccg cgtcagccag aacagcttcg ccgggacaca ctttcgatac cagccgtctt
     2941 tggccccacc tgggcgatcc acacagcagc cgcctgatat cgtcaatgat tccggcgaca
     3001 tcgacaggcg ccatcgcagc gttatgcctg gccgccatcg acaggtaatc gcgcagcagc
     3061 atgtccagcc gattgatctc gctgccaaga ccggcaaagg tggccacatc gtcctggtcg
     3121 gtggcatccc tggacatggc ctccaaggta gccccaatgg ccagaatggg gttcctgatt
     3181 tcatgagcta cctgggcacc cagttcaccc atcgttgcca gcctttcacg acggacaaga
     3241 tgctcgccgc ccgccttgtc caagggtgcc cattgctcac gaatcctcac aagataacca
     3301 agctggtttt catccgcatc gacaagcaga agcgcctcga cgtaaccggg aaagcattcg
     3361 ccgtcctttc gacgcaacag ggcgcattct tcgtaataac cgcttgtcag atccatgatc
     3421 tgcttgtcca gaaggtcgcc gatatcttcc gcgtcgcgaa aaatctcggc aatcgacaaa
     3481 ccggacatgc cgccatcgtc gaaaccgaaa agccggtcgg cggcctcgtt ttccgaagtg
     3541 atgcagaaat ctggaccgac cagcacgaca ggcgaggcct cgcccagcgc aagacgcaca
     3601 agctgatggg tcgccattcc ctggtgtgcg cgaatctcat gaaacgaagc aagcgcgaga
     3661 tcaccggcat ccgtatgaat gcgggaaacg gacacctcga tcctgatgtc gaaaccggta
     3721 tccttcaggc ggacgttgtc ttctggaacg aacctgaagg gaactatgcc ttcgtcgtcg
     3781 gacttcagga aggaaactac ctgtcgccga acctgatcca caaatccggg cgaaaactcc
     3841 attgcgggca caagatccct taatacaaac aggaaccccc cgtcggcgcc gaccgttacc
     3901 cccgagttat aaccgacgat acgaccgccg gcgtccatca gaaagcgtat cagtgcggta
     3961 ttttcacgga ttacaccaaa gtcggctccc tggggaaaag tggaggcccg catgtgcatg
     4021 tccaatatct cgccgacccg ctgggcatac cgaatcagaa gttcacgctg ctgcggactg
     4081 aacgggttga ttcccggctt ctcaaatatc agcacgccga acgaaaggct tccggaaatg
     4141 acgggaacaa gaaaaaagcg atgatccccc agaaggtcca tcgcagcctc gacaccctcg
     4201 tccggcatca gttccggacc gatgatctcg gcaagcgaag gggtttccat ggcagtccgt
     4261 tccttccagg acctatacgc aagtgccgac gtagaaacag cctttaatgg aaacgccacg
     4321 ttctgaacgg caccaaccag gttttcgacc tcgccgatct ggttccggat ggcagccatc
     4381 gtggcgacca cgaccgtcgc ggtctgggcc ttttcgtcca tcagcaccat gttggccgaa
     4441 gtacagccca gcaacttggc cgccccacgc agaatagacg tcaggacatc aagaataccg
     4501 ccggttggtt cgccagccat caggacccac cccatcgata cagatgcgat gccggctgac
     4561 gcgaaaccgt gcgtcttgcg gcaatcggtc cgtaatgata ccttaatccc cacgtcccgg
     4621 ttcagtttcg gcaccttccg gagcaaatca tatgaagaaa tccagcttcc tttttctttc
     4681 ggcaattatc acggttgccg ccacgatcca gggttgcggt ggtgcaactg cgtctgggga
     4741 cacgattgtt gataactcgc cggccgagct tcccgaccag gacgcacaag atgtccatgc
     4801 gtccgatccc ggagccgttc cggatgtttc cgacgttttc gacgagaacc tgcccgagga
     4861 tggattgtcc gagacacact ccgatgccgt aacggatatt atcattccgg atcccatttc
     4921 cacgattggc gcattcaggt tcgacaacat tgaagcgact tccggccaga aagatctgtt
     4981 cacaacactg ctgttgaacg aacgccacgt tactgccgtg aaccgctgcg gattgatggg
     5041 catcgtgttt aacgacgtgg tcgcggacgg cctgggcggg ttcgtttcat cgctggtgtt
     5101 ttcggaagtc aacctttcat caccacctgg cgaagacgaa ctggtatcca gggtaaccct
     5161 gcgttccttt tcggcatctt catcgccggc acgacacatg gccacgcttg catacgaatc
     5221 ggactgcacc ccgattgtca tcgtgcagac tgaagatgga ttccagttca cgcaagtcgg
     5281 cggcggccag attactttta acgaacttga tgacgatacc gattaccagg atatcgaatc
     5341 cctgggcgcc cgggttggcc tggacaaaaa gatccacatg atgttccgcg cacgtcccgg
     5401 tgccggtgcc accatgcagt tcttcgacgc gaccttcaac ggttccggat ttgacgttgt
     5461 cgcaatgcca aacccggaca ccgccctggt ttccacgatc gacgccgccg caggaaatga
     5521 cggccgtgcg gtggtgtgct acctgaagga cggcaccgac ggacaggaag tctgggttgc
     5581 cagattcaac ggcacgacat gggatcggga aagggtggcc ggcgtcgtgc cttcccagct
     5641 acggtggatt gcattcacaa tcggaagtta tggccgggaa agcatcgcct gggcaaacgt
     5701 cgcgaccaat gacgaaggcc tgatactttc agtagatctg aagcacacgt accgggatct
     5761 tgacaagact ttcaagacgg aagccgtcgc ctttgaaaac aacgggtacc agggtggtgg
     5821 tggaaagagg ttcaccgggg ccttgcccag aatccttgtt gacggatccg gcaggccgca
     5881 catactcttt tccgacatag caatcgaaac gtccggaacc agccagacca tactgaccgg
     5941 ccagctcagg tacgcctggc gtcgcaacat gaaatggaac gaaacaacga tcttcgaaca
     6001 gacgccggat caggacggga aagacgcttt cattgagttc agggatttcg ggacttcgcc
     6061 ggacggaacc gcggctggaa tagcggcact tgaagtcgtt gcttccgatt ccaccttcca
     6121 gctttcgacc atcgggattt cggtcgtcaa cgtgaagtaa cgccagttca agccttgacg
     6181 tgggaatcca gccacgtttt cagatcctga acgacttcaa cccggttcgt ttcattcaac
     6241 atctcgtgcc tgccgccttc gtaaagccgc atctgcagat cattgatgcc gtacttgcga
     6301 tacctgtcat gaagggcctt cacgcccttt ccataaccgc cgaccggatc agccgaaccg
     6361 gcaaatatat atatcggaag ggtgcacgga atcctctttt cgtgggccgg ctgccagata
     6421 tgcttcaaca gcatcaacat ctgaaggtaa aagctgttcg ggaagtcggc accgcattcc
     6481 ggatcctcga agtgtgccct taccaccgaa gtatcgcggg acagccagtc caggccggta
     6541 tcggtgtcgt gaagcccctt gttgtacggc cccagggaaa gctgccacag cagctgggcc
     6601 tttgtctctc cgccgcgaat cgcacacacc atccgcgcca ggacaagccc gatggccact
     6661 aacggattgg agccgttggt accggaaagg acgcatccat cgaccctgtc gccgaacgac
     6721 tggatatacg cctgggccag gaacgacccc cacgaatggc ccataaggaa tacggggacg
     6781 ccaggcagtt ccgccctgat ggtcgccgta agctgggtca ttgccgacag aaccgcctgc
     6841 cagcccggct cgccaagcct gcccctgaca ttatccacca gcgtctttcc gtgacccctg
     6901 tggtcgttgg catataccgc ataaccagcc gcggtcatgt cccgggcgaa agcctggtaa
     6961 cgataggccg gttcctgcat cccatgggaa acctggataa ccgcctttac atccccatcg
     7021 ggaagccact tatgaacatg aaccggaaga ccgtgactgt cgttgaaata aaactcggat
     7081 gactgcatgt gatgccccgc gtcaaaagat ctgcaggaca atcataattc gaaatgacaa
     7141 tatgttcagc agtcaagatg ggtttcgatt tcccacggat gcggtcgcga tcgcatttcc
     7201 tcgacttccg ccttcctgtg gatggaaatc caatccgaaa tcaaggactt gctgaagact
     7261 ccaccttctt caaggaaggc gtggtcggac tcaagcgcgt caagagcttc cggcaggctg
     7321 ctgggcaatg gcttgatctt cgcggccctt tcctcggccg acatcgcgaa cacgttcata
     7381 tcgt
//
LOCUS       DAOPPA010000105         7283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11341_length_7283_cov_4.169341, whole genome shotgun sequence.
ACCESSION   DAOPPA010000105 DAOPPA010000000
VERSION     DAOPPA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11341_length_7283_cov_4.169341"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKH54_04640"
     CDS             complement(<1..313)
                     /locus_tag="PKH54_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99210.1"
                     /translation="MISSRRGRVARVENTSMNLLDYFKTGSDRPVKSDDPAVIRREFE
                     WRRWSVFLSVTFGYAFFYVARINLAVVKKPLMDEGVFSASELGVIGSVLFIVYAFGKL
                     FN"
     gene            340..654
                     /locus_tag="PKH54_04645"
     CDS             340..654
                     /locus_tag="PKH54_04645"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOC99211.1"
                     /translation="MKHVLCILCAAAVTIAANSAIAAQIYITDHKSQADAKVFVTKFK
                     SEANCIVYESGHASDNTPGVWYYTKFKSSARASIWYTKFKSEADLIVFYTKFKSEARC
                     RY"
     gene            complement(714..2963)
                     /locus_tag="PKH54_04650"
     CDS             complement(714..2963)
                     /locus_tag="PKH54_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99212.1"
                     /translation="MKKCVALLFVAVFVVVSGCGGGDAGEDLPEVAGDAVSDSLSDEG
                     LPDAGGDLREEDTISGDTVGPDAGDDADAPVDAVDALDAAGDATEPDVPFCTNCTESE
                     PLPLPTYAQRQLEYLDACAANNGPGAGNTYGQVCRVARGLEVNTAAIDSGIEDLTLRE
                     DCSDFRAAALVRMLYLDQVTGSLDETNRQKIVDTLHNYKYWLTQPGKDKMCYWSENHQ
                     ILFHSSEYLVGQMFPETVFANANMTGAQHMEHARPLILKWLDLRGATGFAEWHSNVYF
                     NEDIPALVNLADFAQDPEISTKAMMVLDIIAYDMAANYFSGYFATTHGRTYEGELIGG
                     LNDHTTEGGWMLTGLGAWEGNNNFSAVFLATSGKYFVPDIIESIAAATSAGEFEHRQR
                     DSIDILDGPSWNLTYTEDDDIPTWAGFSAIAAPEIINGTLAFVDKYDLWDGFLFGDLP
                     DYLVQMVKQNMGTQDIVTLSEDATVVSRGIALQSINTYVYRTADYQIAAAQDYHPGWW
                     GSQTLMWQATLDREAFVVASLPTSVELDGSAVSVAGDWVGSWFPRATVYRNAGVFQYR
                     ADVIPDLLSGFFKPERCHAYFRRVAFDEVRELGQWVIGRKGDGYLALYSRHPTEWAAD
                     NDYELVIAPADGKTMDNTWLVQMGSASEFGTFEDFVTAVTESTVAFAEDGSVQWDSPS
                     VGMIAVGWNGPFTVDGVEVDLGPYERFDNAYSNVAFGKRVTRMTFGDRTLELDFAKGT
                     RKVLEKYQD"
     gene            complement(3008..5335)
                     /locus_tag="PKH54_04655"
     CDS             complement(3008..5335)
                     /locus_tag="PKH54_04655"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOC99213.1"
                     /translation="MKSLQILLVASVVAFFLTAGCGAVDGDSDVVQDIAVGQDTSGLP
                     DTSGGDVLQPPDITGIDTAVPEVAVSEEQALAYQRHAELIVESADMTYEELFDRWFDR
                     GEYLEQMPFEASTADFMGQIASAYAIDDDAAALLNANGFAVLDNGSSATHPQLYMDIY
                     VRDLPVFITADSIMYAVHKSYDEMLKQLEENALAATLGDILAKTAFGLTKVCQGASGD
                     VLDACGDIDIYLTVARTLLGGFPVEGLYPATAAARDEILAGVAALQPDQIQMFGRPYP
                     CGLCRYDFSQFKPRGHYNDSELLQRYFRSMIWLGRTPMNLTLYDRDLLASVVLLKALV
                     DGGAMDLWQKLDHAIVVFVGTSDNLTPTGFVQVIEDLALNTDLATLPAGAEYVRGVLA
                     AHGVPGSRILSQIMMMDVMSSEPTPIPAEFQLLGQRFVIDSYVFSNVVYDRITWQDTK
                     PQRYMPDPLDVAFVLGYDQALPLLEDEIGTWNYGGNLNVLRTMVSEYDAGFWTESMYN
                     AWLGGLAELAADQTGVAYPAAARTRAWALRSMQAGLASWAELRHDTILYVKQSYTGES
                     CEYPDGYVEPNPGFFDAMKTFAVKSKEMLSTVQFDQNQYAVNRANYWFDNLIYACDNL
                     AAIARAQLDGTPRTPEQTEFIKSTVVNDGMCGGPGFSGWYADLFYDAQTETFNFKPTV
                     ADVHTDPNSTDVLHVATGGANPMVVVVELPGGPRAFVGPVSSYYQFRESGFGRLTDAE
                     WLERYQAAGGFMPRPDWADRFVTRVEPTAPRNYCE"
     gene            5422..6474
                     /locus_tag="PKH54_04660"
     CDS             5422..6474
                     /locus_tag="PKH54_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC99214.1"
                     /translation="MGDLLKLGILGTASIADRIVAGARKSSIVRVSAVAGRDPARTSA
                     WAATRGIPHTFDSYDALLESGAVDAVYIPLPNSMHAEWTVRALSAGLPVLCEKPAATT
                     AYDASRIRDAAIQSGLPVVEAFMYFFHPLYRQVEQMITNGRIGSVKSIESRFSWKCDD
                     PASGPALSELHGGALFDVGCYCVHLSRKIAGREPVSVMAAARNGFIDPGCQGLGFGPL
                     NMVDDMMVGLLDFGQGTAGLFQTAISSFERHAAEITGTDGSIVIDQPWLQDNAPVSIN
                     LLDDNGRHPLRIDPADTYQAELEGFAGIVRECVRNRGLSPAALAALADLCGNAATLFA
                     LLQSAKTGKAVKPAAL"
     gene            complement(6644..6910)
                     /locus_tag="PKH54_04665"
     CDS             complement(6644..6910)
                     /locus_tag="PKH54_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99215.1"
                     /translation="MSLTARLMIVVVTLGSCLALSACDGRTCDQEKRSTCTAKVSRCI
                     NDKCAGKEGEDYNDCFKYLCLSDFCQCLDDVGCAWQETSCDDVI"
     gene            7046..>7283
                     /locus_tag="PKH54_04670"
     CDS             7046..>7283
                     /locus_tag="PKH54_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99216.1"
                     /translation="MKRNCMAAILVTTVLCAVACGGGGNDTDTSTANDAGSDVMVDSG
                     TDAGTDAQIDSRVDVEGDAQDVAVDTTDVEGDATD"
BASE COUNT         1496 a         2260 c         2123 g         1404 t
ORIGIN      
        1 cgttgaacag cttgccgaac gcatacacga tgaacaggac ggaaccgatt acgccaagct
       61 ccgacgcgga aaacacgccc tcgtccatca ggggcttctt cacgaccgcc agattgatgc
      121 gcgcgacgta aaagaacgcg taaccgaaag tgaccgacag gaacaccgac cagcggcgcc
      181 attcaaattc gcgcctgatg acggcggggt cgtcactctt gaccggtctg tccgaaccgg
      241 tcttgaaata gtcaagcagg ttcatcgatg tgttctccac gcgggcgaca cgtccgcggc
      301 gtgatgatat catccggaaa tcggaaacgg ggatcagcga tgaagcatgt tctgtgtatt
      361 ttgtgcgccg cggccgtcac gattgccgca aattccgcaa tcgccgcgca gatctacatc
      421 accgatcaca agtcgcaggc cgatgcgaag gtcttcgtga cgaaattcaa atcggaagcg
      481 aactgcatcg tctatgagtc cggccacgcg tccgacaaca ctcccggggt ctggtactac
      541 acgaaattca agtcgtcggc ccgggccagc atctggtaca cgaaattcaa gtccgaggcc
      601 gacctgatcg tcttttacac gaagttcaag tcagaggcgc gttgcagata ctgattccgt
      661 ggccacggac gtgcttcacg aaccgcggac ggacatcgcg tcccgccggc cgcctaatcc
      721 tggtattttt ccaaaacctt cctggtacct ttcgcgaaat ccagttccag ggttctgtcg
      781 ccgaaagtca tccgggtgac gcgcttgccg aacgccacgt tcgaatacgc gttgtcgaac
      841 ctttcgtacg gccccagatc gacttcgacc ccgtcaaccg tgaaggggcc gttccagccg
      901 accgcaatca tcccgaccga tggggaatcc cactgaaccg acccgtcctc ggcgaacgcg
      961 accgtcgatt cggtaaccgc cgtaacgaag tcttcaaacg tcccgaactc ggacgcggag
     1021 cccatctgca ccagccaggt gttgtccatc gtcttgccgt cggccggtgc aatcaccagt
     1081 tcgtaatcgt tgtccgccgc ccattcggtc ggatggcgcg aataaagggc caggtacccg
     1141 tcacccttcc gaccgataac ccattgaccc agttcacgga cttcgtcaaa cgccacgcgc
     1201 ctgaaatacg cgtggcacct ttccggcttg aagaacccgg acagcaggtc gggaatcacg
     1261 tcggcccggt actgaaacac gccggcatta cgatagacgg ttgcccgcgg gaaccacgac
     1321 ccgacccagt ccccggccac gctgaccgcg ctgccgtcca gttcgaccga tgtcggcagc
     1381 gaggcgacca cgaacgcctc gcgatcaagc gtcgcctgcc acatcagcgt ctgggatccc
     1441 caccagcccg ggtgatagtc ctgggccgcc gcgatctggt aatcggcggt tcgatacacg
     1501 tacgtgttga tcgattgcag cgcgatcccg cgcgaaacga cggtcgcgtc ttcagacagc
     1561 gtgacgatgt cctgggtgcc catgttctgc ttgaccatct gaaccaggta atccggcaga
     1621 tccccgaaaa ggaaaccgtc ccacaggtcg tacttgtcaa cgaacgcaag cgtgccgttg
     1681 atgatttccg gggccgcgat cgctgaaaat ccggcccagg tcgggatgtc gtcatcttcc
     1741 gtgtatgtca ggttccagga aggtccgtca aggatgtcga tgctgtcgcg ctggcgatgt
     1801 tcaaattcgc ccgcggaggt ggcggccgca atcgattcga tgatatcggg cacgaaatac
     1861 ttcccgcttg tcgccaggaa caccgccgag aagttgttgt tgccctccca ggcgccaagc
     1921 ccggtaagca tccagccgcc ttccgtcgtg tggtcgttca ggccgccgat cagttcgcct
     1981 tcatacgtgc gcccgtgggt ggtcgcgaag tatccagaaa agtaattcgc ggccatgtcg
     2041 tacgcgatga tgtctaaaac catcatcgcc ttcgtggaaa tttccgggtc ctgggcgaaa
     2101 tcggccaggt tgaccagggc cgggatgtcc tcgttgaaat acacgttgct gtgccattcc
     2161 gcgaatccgg tcgccccgcg caggtcaagc cacttcagga tcagcggccg cgcgtgttcc
     2221 atgtgctgtg cgccggtcat gttcgcgttg gcaaaaacgg tttcgggaaa catctggccg
     2281 accaggtatt cggacgaatg gaaaagaatc tggtggtttt ccgaccagta gcacatcttg
     2341 tccttgccgg gctgggtcag ccagtacttg tagttgtgca gtgtatcgac aatcttctgc
     2401 cggttcgttt cgtccagcga ccctgtcacc tgatccagat atagcatcct gacaagcgcg
     2461 gccgcgcgga agtccgagca gtcctcgcgc agggtcagat cctcgattcc cgaatcaatc
     2521 gcggccgtgt tgacttcgag tcccctggcc acccggcata cctgcccgta ggtattgccg
     2581 gcccccggac cgttgttcgc ggcgcaggca tcaaggtact caagctggcg ctgggcatac
     2641 gtcggcaacg gcagcggttc agactcggtg cagttcgtgc agaaagggac gtccggctcg
     2701 gtcgcatcgc cggccgcatc caacgcatcc accgcatcga ccggcgcgtc cgcatcgtca
     2761 ccggcgtccg gcccgacggt gtcgcctgaa atcgtgtctt cttcgcgcaa gtcgccgccc
     2821 gcatccggca gtccttcatc ggacaacgaa tcggaaacgg catcgccggc cacctccggc
     2881 agatcttcac ctgcatcccc gccgccacag ccggacacaa ccacgaaaac cgccacgaac
     2941 agcaacgcaa cgcacttctt caccggacac ctccagatgg tcgggacacc ccgaacgggc
     3001 aatgatatca ctcgcagtag tttcgcggtg ctgtcggttc aacccttgtg acgaaccggt
     3061 cggcccagtc cggacggggc atgaacccgc ccgccgcctg gtacctttca agccactccg
     3121 catcggtcag gcggccgaag ccggactcgc ggaactggta ataggacgat accggcccga
     3181 cgaatgcgcg cgggccgccg ggcaattcga cgacgacgac catcgggttg gccccgccgg
     3241 tcgccacgtg cagcacgtcg gtgctgttcg ggtcggtatg gacgtcggca acggtcggct
     3301 tgaaattgaa agtctcggtc tgggcgtcat agaacaggtc ggcgtaccac ccggaaaatc
     3361 ctggcccgcc gcacatgccg tcgttcacga cggtcgattt gatgaactcg gtctgctcgg
     3421 gcgtcctggg cgtgccgtcg agctgggccc tggcgatggc ggccaggttg tcgcatgcat
     3481 agataaggtt atcgaaccag taattggcgc ggttgaccgc gtactggttc tgatcgaact
     3541 ggacggtcga aagcatctct ttcgacttga ccgcgaaggt cttcatcgcg tcgaagaatc
     3601 ccgggttcgg ctcgacgtaa ccgtccgggt actcgcacga ttccccggta tatgactgct
     3661 tcacgtacag gatggtgtcg tggcgcagtt cggcccacga ggcaagcccg gcctgcatcg
     3721 agcgcagcgc ccaggcccgt gtgcgggcgg cggccgggta cgcgacgccg gtctggtcgg
     3781 cagccagttc tgccaggccg ccaagccacg cgttgtacat gctttcagtc cagaacccgg
     3841 cgtcgtattc ggacaccatc gtgcgcagca cgttcaggtt gccgccgtaa ttccatgttc
     3901 cgatttcgtc ttccagcagt ggcagtgcct ggtcgtaccc aagcacgaac gccacgtcca
     3961 gcgggtcggg catgtagcgc tgcggtttcg tgtcctgcca tgtgatacgg tcatagacga
     4021 cgtttgaaaa tacatagctg tcgatgacga accgctggcc aagaagctgg aattcggccg
     4081 gaatcggcgt cggttcggac gacatcacgt ccatcatcat tatctggctc aaaatccttg
     4141 aaccgggcac gccgtgcgcg gccagcactc ccctgacata ttcggcccct gccgggaggg
     4201 tggccagatc cgtgttcagg gcgaggtcct cgatcacctg gacaaacccg gtcggcgtca
     4261 ggttgtcgct ggttccgacg aacacgacga tagcatgatc cagtttctgc cacagatcca
     4321 tcgcgccgcc atcaacaagc gccttcagca gcaccacgga cgccagcaga tcgcgatcgt
     4381 acagggtcag gttcatcgga gtgcgcccaa gccagatcat cgaccggaag taacgctgaa
     4441 gcagttcgga gtcgttgtag tggccgcgcg gcttgaactg cgaaaagtcg tatcggcaca
     4501 acccgcacgg gtatggacgc ccgaacatct ggatctggtc gggctgcagt gccgccacac
     4561 cggccaggat ttcgtcccgc gccgccgcgg ttgccggata aagcccttca acagggaaac
     4621 cgccaagcag cgttctcgcg accgtcagat agatatcgat gtcgccgcat gcgtccagga
     4681 cgtcaccgga agcgccctgg caaaccttag tcagaccgaa ggcggtcttt gccaggatgt
     4741 ccccaagcgt ggcagccaga gcgttttcct cgagctgctt caacatttca tcgtatgact
     4801 tgtgaaccgc gtacatgatc gaatcggcag tgatgaaaac cggcagatcc ctgacataga
     4861 tgtccatgta aagctgcggg tgggtcgccg aactgccgtt gtccagaacc gcgaagccgt
     4921 ttgcgttcag aagggccgcc gcgtcgtcgt caatcgcgta agcagatgcg atctggccca
     4981 tgaaatcggc cgtggacgcc tcgaaaggca tctgttccag gtattcgcca cgatcaaacc
     5041 accggtcgaa aagctcttcg tacgtcatgt cggcggactc gacaatcaat tcggcgtggc
     5101 gctggtacgc aagcgcctgc tcctcggaca cggccacctc gggaaccgcc gtgtcgatgc
     5161 cggtgatgtc cggcggttga agcacgtcgc cgccagaggt gtcagggagg ccagaggtgt
     5221 cctgaccgac cgcgatgtcc tggacgacat cggaatcgcc atcgacagca ccgcatccgg
     5281 cagtcaggaa aaacgcgaca acggacgcaa caagcaatat ctgcaacgat ttcatcctgg
     5341 ccacccgcga tgaaagattc gcaaggatta tacaacttaa cggttgtgac atagaatccc
     5401 gccgggctgg gggcaagtaa catgggcgat ttgctgaaac ttgggatact gggaacggca
     5461 tcgattgccg acaggattgt cgcgggtgcc aggaagagct cgattgtaag ggtgtcggcc
     5521 gtcgccggcc gcgatcccgc caggacatcg gcctgggcgg cgacccgcgg aattccccac
     5581 actttcgatt cgtacgacgc gctgctggaa tccggtgcgg tcgatgcggt ctatatcccg
     5641 cttccaaaca gcatgcacgc cgaatggacg gtgcgcgcgc tttcggcggg ccttcccgtt
     5701 ctttgtgaaa agccggccgc aacgaccgcg tacgatgcct cgcggatcag ggatgccgcg
     5761 attcagtccg gacttccggt cgtcgaggcg ttcatgtact ttttccaccc gctgtaccgg
     5821 caggttgaac agatgatcac caatggcagg atcggaagcg tcaaatcgat tgaaagccgg
     5881 ttcagctgga agtgcgacga cccggcctcg gggccggccc tgtcggaact tcacggcggc
     5941 gcgctgttcg acgtcgggtg ctactgcgtc cacctttcca ggaagattgc cgggcgcgaa
     6001 ccggtttcgg tcatggcggc cgcaagaaac ggcttcatcg acccgggctg ccagggcctg
     6061 gggttcggac cgttgaacat ggtcgatgac atgatggtcg ggctgcttga tttcgggcag
     6121 ggaacggccg gcctgttcca gacggccatt tcatcattcg agcggcatgc ggccgaaatc
     6181 accgggactg acggttcaat cgtcatcgac cagccgtggc ttcaggacaa cgccccggta
     6241 tcgatcaacc tgcttgacga caatggaagg catccgttaa ggatcgatcc cgccgacacg
     6301 tatcaggccg aacttgaagg atttgccggc atcgtccggg aatgcgtccg gaatcgcggc
     6361 ctgtcgccgg ccgcgctggc cgcgctggcc gatctatgcg gaaatgccgc gaccctcttt
     6421 gcattgctgc agtcggccaa aaccggaaag gcggtcaagc cggccgctct ctgaagcccg
     6481 ccgcgacaga ttcacgacac gcgcatgcac cgacgtaaca cgaaagcacc gcgggaattc
     6541 gtgaaggaac tcagtcataa aacgccgtca cacgcggcgc tcacgcaagg ccattaccgg
     6601 tattatcgca aggtaatttg attgaacttc attcaatgct ctgtcaaatg acgtcgtcgc
     6661 aggacgtttc ctgccacgcg cagcccacgt cgtccaggca ttggcagaaa tcggaaaggc
     6721 acaggtactt gaaacagtcg ttgtaatcct ccccttcctt gcccgcgcat ttgtcgttta
     6781 tgcacctgga aaccttcgcg gtgcatgtcg aacgcttttc ctggtcgcat gtgcgaccgt
     6841 cgcatgccga cagtgccagg cacgacccca gggtaaccac gacgatcatc aatctggctg
     6901 tcaaggacat cctttccgac ctgcaaaaac ctgccgcaac tttacagtaa ccgacacttt
     6961 tcatgaagcg ggaatcggta ttggaacctt catttcccga tcgtataatg ccgacatatc
     7021 ttgaacgtcc ctccgggagg caatcatgaa gcgcaactgc atggccgcga ttcttgtcac
     7081 gacagtcctg tgcgccgtcg cgtgcggcgg cggcggcaac gatacggata cttcaaccgc
     7141 gaacgacgca ggatccgacg tgatggtcga ttccggaacc gacgctggca ccgacgcgca
     7201 aatcgattcg cgagtggatg tcgaaggcga cgcccaggat gtcgcggttg acacgacgga
     7261 tgtcgaaggc gatgccacgg atg
//
LOCUS       DAOPPA010000106         7271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11374_length_7271_cov_3.816796, whole genome shotgun sequence.
ACCESSION   DAOPPA010000106 DAOPPA010000000
VERSION     DAOPPA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11374_length_7271_cov_3.816796"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKH54_04675"
     CDS             <1..552
                     /locus_tag="PKH54_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC99217.1"
                     /translation="KEFRGDDVPVVCVDWDQAAAYANWVGGRLPTEAEWEYAASSGGQ
                     IKEYPWGAKAATCEKAVMRDELFQKKSKSPKPDGCGLNRPWPVCSKLSGNTVQGLCDM
                     AGNVWEWVSDWYWISGPAGIFEWPYGYSSKAQTNPTGPSSGTYRVIRGGAWNNDSFNL
                     RINKRESVHPGRKSNAVGIRVVR"
     gene            complement(738..2330)
                     /gene="groL"
                     /locus_tag="PKH54_04680"
     CDS             complement(738..2330)
                     /gene="groL"
                     /locus_tag="PKH54_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415963.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOC99218.1"
                     /translation="MAAKDLAFSADARKRILNGVNILADAVTVTLGPRGRNVILEKSW
                     GSPLVTKDGVTVAKEIELEDRFENMGAQMVKEVASKTSDKAGDGTTTATVLARAIYQE
                     GIKLVTAGINPMHLKRGIDAGVEAVCGELNKMAIPTRGRTEIAQVGTISANHDTEIGD
                     IIADAMDKVGKDGVITVEEAKGTDTTLDLVEGMQFDRGYISPYFVTNAEKMETVMDDP
                     YILLYDKKVSGLHDVLPLLETVAREGRQLVIVSEDVEGEALAALVVNKLKGVLKVLAV
                     KAPGFGDRRKAMLDDLAILTGGKVISEELGMKLENVRIGDLGRCARVIANKDNTTIVD
                     GAGKPENIQARIGQIRRQIEESTSDWDREKLQERLAKMTGGVAVIHVGAQTEAEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALVRAMPVLDTVNFDDDRRFGIDILRKAIAEPL
                     KRIAGNAGVDGGVVLQKVIEGTGNFGYNAQTGAYEDMVAAGILDPKKVTRLALTNAAS
                     IAGLMITTEAGIVEAPKEDDKK"
     gene            2592..2864
                     /locus_tag="PKH54_04685"
     CDS             2592..2864
                     /locus_tag="PKH54_04685"
                     /inference="COORDINATES: protein motif:HMM:NF021083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HOC99219.1"
                     /translation="MVDRDKFGERHACFECGCKFYDMKRNPPTCPRCGTDVSKPPEVA
                     VSVVVPTDDDIIEDDDDATATLEDEELPIEDLDSTEEMSEEEMGDE"
     gene            2880..3764
                     /gene="mtnP"
                     /locus_tag="PKH54_04690"
     CDS             2880..3764
                     /gene="mtnP"
                     /locus_tag="PKH54_04690"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOC99220.1"
                     /translation="MIGIIGGTGLYFLDSLEDVREIDVATPFGKPSDLITVGRFHGNE
                     IAFLPRHGVGHRFLPTEVPYRANIWAFKYLGVNRIFSVSAVGSMRNDVRLSVPVIVDQ
                     MIDRTIHRQSTFFGNGLVAHVSMADPFCPSLRRLLLESAARIGMEVVDGGAYVCIEGP
                     QFSSRAESQWYRDIGSGSNHRDVRVNVIGMTNATEAKLAREAEICYATIALPTDYDCW
                     HEEEDDVNCASVVERLQAGTTRARELILAALRQIDAAGPCGCGDSLCHAFLTRRSLVP
                     PRTLEMLAPITDRYFPKD"
     gene            3835..4767
                     /locus_tag="PKH54_04695"
     CDS             3835..4767
                     /locus_tag="PKH54_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC99221.1"
                     /translation="MSLLVVGSVAYDVIELPGREAVEVLGGSATWFSTAAAFLARPVI
                     VAVVGSDFLEDDLAFLRGRGIDTSRIERADGVTFRWHGRYHSDMIGRDSLRTELGVFA
                     DFCPVVPEDLRDCDVLCLGNIKPELQKYVLMQTRNARFVALDTIECWISQYRDQLVSM
                     MPRTDLLFLNDDEVRQLSGQRNIFSAAKSILSMGAKALVIKKGEHGAILFDSRGIKLY
                     PAYPVESVVDPTGAGDSFAGGTLAYLDRVGSFDVDDVRRALAVGTVMASFCVEGFGPR
                     AFARIRPSDLVSRMTEYVKMTSLEGERIISDISL"
     gene            4781..5620
                     /gene="asd"
                     /locus_tag="PKH54_04700"
     CDS             4781..5620
                     /gene="asd"
                     /locus_tag="PKH54_04700"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOC99222.1"
                     /translation="MSDTVRRLLYRAVSSKVQSRIAGRISDAKLPTPVLERLIDLWVA
                     HFGVNLDEAIIPPCGFQSFNDFFTRRLKTGARDFPADTGVMASPCDGMVQTAGAISGG
                     MVFQAKGIEYRLADLLADSGEAARFEQGHFLTIYLNPGDYHRVHFPIAAEVRKVRHIP
                     GGLLSVAPRVVELFNEVFGTNERVTGLLESDNGMVGMVMVGATTVGRISLSFSDVVTN
                     KGKAHGICVFGPPLRRLRGDEYGAFNLGSTVILLVEKDRWVPVHPGVGQKIRLGQAIF
                     EPK"
     gene            complement(5632..6204)
                     /locus_tag="PKH54_04705"
     CDS             complement(5632..6204)
                     /locus_tag="PKH54_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99223.1"
                     /translation="MSIQSMLRSFACVGVVVLLAGCSKQESAGPVVELNLPPKPDLHA
                     KQIPEKYDDGTLTVTGVVKNAAQLNNNQVKVRGKVVKIETCAVGEVCTGEAAVTLTDD
                     DPKSTRTLAVMDPSEGLYHDLAKEFQVGALVTVEGKLSTWSPTGRTINMDGILLLDKP
                     KAPECAEGPEGDECRKAAETAKAAEAARSK"
     gene            6496..>7271
                     /locus_tag="PKH54_04710"
     CDS             6496..>7271
                     /locus_tag="PKH54_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOC99224.1"
                     /translation="MLLDGLYGLFSNDLAIDLGTATTLVYVRGKGIVSCEPSVVAVMK
                     DHRNSRKVLAVGREAKEMLGRTPGGIEAIRPMKDGVIADFEVTEAMLRYFITRAHNRR
                     TLVRPRIVICVPHGITEVERRAVRESAESAGAREVFLMEEPMAAAIGAGLPITEPCGN
                     MIVDIGGGTTEVAVISLSGVVYSRSVKVGGDKMDDAIREHLKRKYNLLVGDRTAERVK
                     IEIGNAYPLPEVLYLDVKGRDQVAGIPKQVTLNSDEIREAL"
BASE COUNT         1317 a         2166 c         2222 g         1566 t
ORIGIN      
        1 aaggaattca ggggtgacga cgtgccggtg gtgtgtgttg actgggatca ggctgcagcc
       61 tacgcgaatt gggtcggcgg ccggttaccg acggaagctg aatgggagta tgcggccagc
      121 agtggtggtc agatcaagga atacccgtgg ggagccaaag cggcaacttg cgaaaaggca
      181 gtcatgcggg atgaactttt tcaaaaaaaa tcgaaaagtc caaagcccga tggatgcggc
      241 ttgaatcgcc cctggcccgt ttgttcaaaa ttgtcgggaa atacggttca ggggctttgt
      301 gacatggcgg gcaatgtctg ggaatgggtt tccgactggt attggatcag cggtcctgcg
      361 gggatttttg aatggccata tggctattcg agcaaggcgc agacaaatcc cacgggccca
      421 tcgtccggta cttaccgggt tattcgtggc ggagcctgga acaatgattc tttcaacctg
      481 cgtatcaaca aacgagagag tgtgcatccc ggccgcaagt caaatgcggt cggaattcgg
      541 gtcgtcagat agttttccct ggttttcttt gtcctttccc accgacttca gtcctccaga
      601 aaagcgaatg tgcccatgca ggggcagggg tactggttcg cggcgggcgg atgtaatccg
      661 cccggtaggg gcgaatgaga attcgcccgc acaaaggcaa aggcaaaggc aaaggcaagg
      721 gcagggacag ggccggatta cttcttgtcg tcttccttgg gggcttcgac gatgccggct
      781 tcggtcgtaa tcatcaggcc ggcaatggac gcggcgttgg tcagggcaag ccgggtgact
      841 ttcttcggat ccaggatgcc tgccgcgacc atgtcttcat aggcgccggt ctgggcgttg
      901 taaccgaagt ttccggtgcc ctcgatgacc ttctgcagaa ccacgccgcc atcaacgccg
      961 gcgttgcccg cgatcctctt caacggctcg gcgatcgcct tgcgaaggat gtcgattccg
     1021 aacctgcggt cgtcgtcgaa attcacggtg tcaagaaccg gcatcgcgcg aaccagggcg
     1081 acgccgccgc ccgggacaac gccttcctcg accgcggcgc gggtcgcgtg aagggcgtcc
     1141 tcgacgcggg ccttcttttc cttcatttcg gcctcggtct gggccccgac gtgaatcacc
     1201 gcgacaccgc cggtcatctt cgccagacgt tcctgcagct tctcgcgatc ccagtcgctg
     1261 gtcgattcct cgatctggcg gcggatctgg ccaatcctgg cctggatgtt ttccggcttg
     1321 ccggcgccgt caacgatcgt cgtgttgtcc ttgttcgcga tcacgcgggc gcagcggcca
     1381 aggtcgccaa tccgcacgtt ttccagcttc attcccagtt cttcggaaat caccttgccg
     1441 ccggtcagga tggccaggtc gtccagcatc gccttgcgac ggtcgccgaa tccgggcgcc
     1501 ttgacggcca ggaccttcag aacccccttc agcttgttga cgaccagggc ggccagtgcc
     1561 tcgccctcga cgtcctcgga cacgatgaca agctggcgtc cctcgcgggc aaccgtttcc
     1621 agcagcggaa gcacgtcgtg cagcccgctg accttcttgt cgtaaagaag gatgtacggg
     1681 tcgtccatga ccgtttccat cttttccgcg ttcgtcacga agtacgggct gatgtatccg
     1741 cggtcgaact gcatgccttc gaccagatcc agcgtcgtgt cggtgccctt ggcttcctcg
     1801 accgtgatga ctccgtcctt gccgaccttg tccatcgcgt ccgcgatgat gtcgccgatt
     1861 tcggtgtcat ggtttgcgga aatcgtgccg acctgggcga tttcggtgcg gcccctggtc
     1921 gggattgcca tcttgttcag ttcgccgcag acggcctcga caccggcgtc gatgccgcgc
     1981 ttcaggtgca tcgggttgat gccggcggtc accagcttga ttccttcctg gtaaatcgcg
     2041 cgggcaagca ccgtggcggt cgtcgtgccg tcgccggcct tgtccgacgt cttggacgcg
     2101 acttccttga ccatctgggc gcccatgttt tcgaacctgt cttcaagctc gatttccttc
     2161 gcgacggtga cgccgtcctt ggtcacgagc gggcttcccc aggacttttc aaggattacg
     2221 ttgcgtcccc gcggccccag ggtgacggtg accgcgtccg ccagaatgtt gacgccgttc
     2281 aggatgcgct tgcgcgcgtc agccgaaaat gcaagatcct tcgctgccat ttcatatctc
     2341 cgtggtttgt accttgacct tcatccgtgg gaatcaatcc ccggtcacct ttcaaacagg
     2401 cggttcgacc gccccgcaat ataaatacgc ccgggcgcag gtcaaggggt tccggcgaga
     2461 cgggcatcac gaaaatgttg acgacgaaca tcatctcgtg ttcgatatgc ttgacattac
     2521 gtccgtgatt gtttacctag cgcgtccgcc ggattttgtc cggcgtatct tcgaaacttt
     2581 tgaggtttgg gatggtcgat agagacaaat tcggcgaacg acatgcttgt ttcgagtgcg
     2641 gatgcaagtt ttacgacatg aagcgcaacc cgcccacctg tccccgatgc ggcaccgacg
     2701 tgtccaagcc gcccgaggtt gctgtgtccg tggtcgtccc gaccgatgac gacatcatcg
     2761 aggatgatga cgacgcaacc gcgaccctcg aggacgaaga actcccgatc gaggatctcg
     2821 actcgaccga ggagatgtcc gaagaggaaa tgggcgacga gtaaccggcg ggggttccga
     2881 tgatcggcat aatcggcgga acgggccttt atttccttga ttcgcttgaa gacgtcaggg
     2941 aaatcgatgt cgccacgccc ttcggcaagc caagcgacct tatcaccgtg ggcaggttcc
     3001 acggaaatga aatcgctttc cttccaaggc acggtgttgg ccaccgcttc cttccgaccg
     3061 aggtccctta ccgggcgaac atctgggcgt tcaagtacct tggcgtgaac cggatctttt
     3121 cggtcagcgc cgtcggaagc atgcgcaacg acgtgcgcct ttcggtgccg gtaatcgtgg
     3181 atcagatgat tgatcgcacc atccaccgcc agtcaacgtt ctttggaaac ggcctggtcg
     3241 cgcacgtcag catggcggat ccgttctgcc cgtcgttgcg ccgtcttctg ctggaatcgg
     3301 ccgcccgaat cggcatggaa gtcgtcgatg gcggcgctta cgtgtgcatc gagggtccgc
     3361 agttttcgtc gcgcgcggaa tcccagtggt accgcgacat cggaagcgga tcgaatcatc
     3421 gcgacgtccg cgtcaacgtc atcggcatga cgaacgcgac cgaggcgaaa ctggcccgcg
     3481 aggccgaaat ctgctacgcc accatcgcgt tgccgaccga ttacgactgc tggcacgagg
     3541 aagaggacga cgtgaactgc gccagcgtcg tcgagcggct ccaggccggc acgaccaggg
     3601 ccagggaact gatccttgcg gcattgcggc agatcgacgc ggccggacct tgcggatgcg
     3661 gcgattcgct ttgtcacgcc ttcctgacca ggcgcagcct tgttccgccc cggacgcttg
     3721 agatgcttgc gcccattacg gacaggtact tcccgaagga ttaggccgcc ggcgcctttc
     3781 ggttgacggt cggataacag ccatgacgtc cagtaaaaag gcaatgaggt tcgcatgagt
     3841 cttcttgttg tcggttcagt tgcgtacgac gtgatcgagc tgcccggaag ggaagcggtg
     3901 gaagtccttg gcggttccgc gacttggttt tcgaccgcgg ccgcctttct tgcgcgtccg
     3961 gtaatcgtcg cggtggtcgg atccgatttc cttgaagacg acctggcctt ccttcgcggg
     4021 cgcggcatcg acacgtccag gatcgagcgt gccgatgggg tgactttccg ctggcacggc
     4081 cgttaccatt ccgacatgat cggccgggat tccttaagga ccgaactcgg ggtctttgcc
     4141 gatttctgtc cggtggtgcc tgaagacctg cgcgattgcg acgtcctgtg cctgggcaac
     4201 atcaagcccg agcttcagaa gtacgtcctg atgcagacca ggaacgccag gttcgtggct
     4261 ctggacacga tcgagtgctg gatttcccag tatcgcgacc agcttgtgtc gatgatgcca
     4321 aggaccgacc tgctgttttt gaacgacgat gaggtccgcc agctttcggg ccagcgcaac
     4381 atcttttcgg cggcgaaatc gatcctttcg atgggcgcga aggccctggt cataaagaag
     4441 ggcgagcacg gcgcgatcct tttcgacagc cgcggaatca agctttatcc cgcctatccg
     4501 gtcgaatcgg tcgtcgatcc gaccggtgcc ggcgattcct ttgccggcgg aacgcttgcg
     4561 tacctggatc gcgtgggcag cttcgatgtc gatgacgtcc ggcgcgcact cgcggtcggc
     4621 acggtcatgg cgtctttctg cgtcgaaggt ttcggtccaa gggcatttgc ccggatacgg
     4681 ccgtccgacc tggtttcgcg catgactgaa tacgtgaaaa tgacgtcgct tgaaggcgaa
     4741 cgtataatct ccgacatatc cctgtgaccg ggaggcgtcc ttgtccgata cagtaagaag
     4801 gctcctttac agggccgttt catcgaaggt tcaatcaagg attgccggcc gcatcagcga
     4861 tgcgaaactg ccgacgcccg tccttgaacg tcttatcgat ctgtgggtcg cgcatttcgg
     4921 ggtcaatctc gacgaagcga ttattccgcc ctgcgggttc cagtcgttca acgatttttt
     4981 caccaggcgc ctgaagaccg gtgcaaggga tttcccggcc gataccggtg tgatggcatc
     5041 gccatgcgac ggaatggtgc agactgccgg ggcgatttcg ggcggcatgg tgttccaggc
     5101 caagggaatc gagtaccggc tggcggatct gcttgccgat tcgggggaag ccgccaggtt
     5161 cgaacaaggc cacttcctta ccatttactt gaatcccggg gactaccacc gggttcattt
     5221 cccgattgcg gcggaagtgc ggaaggtccg gcacatcccg ggcgggctgc tttcggtcgc
     5281 cccgcgtgtc gtcgaactct tcaacgaagt cttcggcacc aacgaacgcg tcaccggcct
     5341 gcttgaaagc gacaacggaa tggtcgggat ggtaatggtc ggagcaacga cggtcggaag
     5401 gatttccctg tcattttcgg atgttgtcac gaacaagggg aaggcgcacg gaatctgcgt
     5461 tttcgggccc ccgctgcgtc gccttcgtgg tgatgagtac ggtgcgttca accttggatc
     5521 gaccgtaatc ctgctggtgg aaaaggatcg ctgggttccc gtccacccgg gtgtcggtca
     5581 gaagatcagg cttggtcagg ccatttttga accgaaataa ttcgagatga tctatttcga
     5641 cctggcggct tcggcggcct tggctgtctc ggcggccttg cggcactcgt cgccttccgg
     5701 gccttccgcg cattccgggg ccttgggctt gtcaagcagc aggatgccgt ccatgttgat
     5761 ggtgcggccg gtcggcgacc aggttgaaag cttgccttca accgtcacga gcgcgcccac
     5821 ctggaattcc ttggcaaggt cgtggtacag cccttcgctg ggatccatca ccgcaagcgt
     5881 cctggttgac ttcggatcat cgtcggtcag cgtcacggcg gcctcgccgg tgcagacctc
     5941 accgaccgcg cacgtctcga tcttgacgac cttgccgcgg accttgacct ggttgttgtt
     6001 caactgtgcc gcgtttttga cgacgcccgt gaccgtcaga gtgccgtcgt cgtacttttc
     6061 cgggatctgc ttcgcgtgaa ggtccggctt cggcggcagg ttcagttcca ccaccggtcc
     6121 cgcactctcc tgcttcgagc atccggccag caggacaacc actccgacac aagcaaacga
     6181 cctgagcatg gactggatgg acatctttga tactccttcg gtaagtacgg aaccacgtgg
     6241 gcccaattat tatcagttaa gtgcgcgccg gtcaaacgaa actggcccgg tgccgcgccg
     6301 gcggcctgtc ggccacatcc gccgtccacg ccacgcgttg ttcccggcgc cgctttggaa
     6361 taatggtccg ggcgaatgcc gtggccgcgt cggccttcgc agattacctt ttgatcattc
     6421 gacgatcccg tgatacactg cacgcccttt ctgcggcgcg gggttgggca caggtctgtc
     6481 ccgcggggga accggatgct gcttgacggg ctgtacgggc tgttttccaa cgatctggcg
     6541 atcgacctgg gaacggctac aactctggtt tatgttcggg gcaagggcat tgtctcgtgc
     6601 gagccgtccg tcgtggcggt catgaaggat caccgcaatt cccgcaaggt tctggcggtc
     6661 ggccgcgaag ccaaggaaat gcttggccga acgcccggcg gcatcgaggc catccgaccg
     6721 atgaaggacg gcgtcatcgc cgacttcgaa gtcaccgagg ccatgctccg gtacttcatc
     6781 acgcgtgccc acaaccgtcg caccctggtt cgtccacgca tcgtcatctg cgtgccgcac
     6841 ggcatcaccg aagtcgagcg ccgcgcggtt cgcgaatcgg ccgaatcggc gggcgcccgc
     6901 gaagtattcc tgatggaaga accgatggcg gccgcgatcg gggccggcct gccgatcacc
     6961 gagccgtgcg gcaacatgat cgtcgatatt ggcggcggca ccaccgaagt cgccgtcatt
     7021 tccctttcgg gcgtcgtcta tagccgttcg gtcaaggtcg gcggcgacaa gatggacgac
     7081 gccatccgcg aacacctgaa gcgcaagtac aacctcctgg tcggcgaccg gacggccgaa
     7141 cgcgtcaaga ttgaaatcgg caacgcgtat ccgcttcctg aagtcctgta cctggacgtg
     7201 aagggtcgcg atcaggttgc cggcattccg aagcaggtca ccctgaattc ggatgaaata
     7261 cgcgaggcgc t
//
LOCUS       DAOPPA010000107         7254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11414_length_7254_cov_4.163217, whole genome shotgun sequence.
ACCESSION   DAOPPA010000107 DAOPPA010000000
VERSION     DAOPPA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_7254_cov_4.163217"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="PKH54_04715"
     CDS             <1..1936
                     /locus_tag="PKH54_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99225.1"
                     /translation="LLTNLRAGRIDTAEVALKQLITLRKQYNIANLSPVAAAFIGYFD
                     PGTTESTPKKRRQLLETAIELAPDYPAVYFKLAKVRLFQGIQNIGPAATIAMDGFERM
                     LRFPRSASILAAESSFYLIGVLLILYIILGLGFLIRYFPMIRHDIGDILPNAETTHFT
                     AAEIRKSRGPLQSFSRGLSNGLLTLLLLVICFAPFVLGAGLLLTAALWMLLVFPYGRL
                     SERIMIVVMTVVAAALPTTGSLTALPGHVNGTAGTLRWSCITEYCPQESRQALELLLD
                     DGVGATGVGDRARHESTIVAVALGRLQFAIGQPDQIPAIRSFMEARKDSLESPLGQVL
                     MGNVIVLDGLHKCPAGRHDTAVMASAMGWYSRALTASPDNEAANRGMAVASGLAGDRK
                     GMELSLQRVLEITKESDLDFMAQVRTLTASSDACDHLVELAGELRPPRPSSNHEIYFD
                     GLSNFELLTRTPPLLPFKSVLLGAVSPQMIPLILAVMLAVGVALVFVNPRRRRAVRCP
                     ACGHVICPSCNRRASGFEHCPICLFDQVRPAFLDPKDVAAMSAFQHRGLAARYWLPAM
                     SIAVPGSGQILGGRPFRGLTMMLLLGLGAMMVITPSAPIIDSFAYIPQQARGLPLLPP
                     ILLVSAYLWSVIDVIITRKR"
     gene            1933..3264
                     /locus_tag="PKH54_04720"
     CDS             1933..3264
                     /locus_tag="PKH54_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99226.1"
                     /translation="MSDDLEIAMSPIRLDVEMHDDQSDGYDDDSSKTRAFNPFETGPA
                     GRQKYPPLFAALAVIYRRNLTGVLTARRDGKDVLSATVEKGRVVSVAHQDLTQDTIPE
                     LLLDTGLVSMKHFKAAVRKADHNNTSIEVELVKANRISMTTAANLLEATLTEILLKVM
                     GERDLEFTFFKYLPAGLRRNCHLPIPFLLKEVSRRAAELPGIMAHFPDDSVGFVRTSQ
                     LDSNPEEYLWEDLDLGSAEKQIYFFVDGHRTIDDLQTASGQSRFSVMRALAALIDTGY
                     VVATVDPPDRSGTNHTFWNLVRALSLVLVLTGLGFLVGTRISPERLPHVSTRYGLRTA
                     AYGSTIMDSIRDRVSGAILSYHLLTSKNDITVDKLLNDGLIDDNDAVVAEQILQAAAE
                     EQAEKERVRKLLIIRELQDREAPAQNSAPETAKAPEPADAGMGAEPSDDTK"
     gene            3251..4246
                     /locus_tag="PKH54_04725"
     CDS             3251..4246
                     /locus_tag="PKH54_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC99227.1"
                     /translation="MIQSNQSIQVDIEFSDINAFMSLCGQGDENVRRLEALLGVVVRI
                     RGNQVSLYGQPDVVKVAQGILKQLYTLCQASHDVTPVDIDRAVAVIEADPAADLADVF
                     ASGVSIGGVPSPVRPRTPAQKGYIRAMRDNDLVISVGPAGTGKTYLAMATALGFLQSH
                     KVKSIVLTRPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDIMDNDRGQRLIDKGVI
                     EIAPLAFMRGRTLSNAFIILDEAQNCTADQMKMFLTRLGCGSRAVVTGDLTQTDLPAG
                     VPSGLEYAVNVLAEVPGIAISRFTDADVMRHPLVRRIIRAWDGLDKGGADGGSRL"
     gene            4227..4697
                     /gene="ybeY"
                     /locus_tag="PKH54_04730"
     CDS             4227..4697
                     /gene="ybeY"
                     /locus_tag="PKH54_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOC99228.1"
                     /translation="MAVHVYNNQRKVKIVRGCFDVAFETALRIAGHADDDVEVTFVSN
                     AVIAELNAQWRGKSGPTDCLSFPFAPDVPMPPEVRAPLGEIVISLEQAAAQGPIHVKY
                     AAAGTHPFISEVVFLFIHSLLHLIGFDHETGPEDERIMFERQEQVFAAVMAEIS"
     gene            complement(4905..6458)
                     /gene="rny"
                     /locus_tag="PKH54_04735"
     CDS             complement(4905..6458)
                     /gene="rny"
                     /locus_tag="PKH54_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOC99229.1"
                     /translation="MVGILLAAIGGIVGGGAIAFLATRATVKSRLEKEKENLDAAIQE
                     SSVKWEAKVQQALSQARNADIRVKALEKELNKVKSDADRKEERLAKREETVDRKMDTL
                     VQRESEIVSREKETVQRESDVKARMAEAEKMTAEARTVLESVAGLSQEEARAMLTERL
                     MEDVKLSAAKSIKMIEEEAKEEAEKRAKKIVGVAMMRYAAEFANERTVSVVQLPSEEM
                     KGRIIGREGRNIRAFEQATGIDVIIDDSPDTVIVSGFNPVRREIARLALEKLVQDGRI
                     HPARIEELVEKTAREVDQNIKETGEDALYQLSLGTMHPELVKLIGRMKYRTSYGQNVL
                     QHSLEVGGLAGLMAAEMGVPVKMARRAGLLHDIGKAIDHEVEGPHALVGANFARKFGE
                     NAEIVHAIASHHNEEKPLTLLAHITAAADAVSGSRPGARRESLENYLKRLGDLEEISN
                     SFAGVNRSYAIQAGREVRVIVENERIDDDGATILARDIARKIEEQLSYPGQVKVCVIR
                     ETRAVDFAK"
     gene            complement(6721..7053)
                     /locus_tag="PKH54_04740"
     CDS             complement(6721..7053)
                     /locus_tag="PKH54_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOC99230.1"
                     /translation="MEKRVVEITVQGQKFVLKTDLDDNALEFISQTANNRIDSISSAA
                     PSFSPHKTALLAVLDLAEELFHEKKNLTDLKENIRTQSTKLLYALNESRNLQSKRTLV
                     GDNPPGEG"
BASE COUNT         1379 a         2249 c         2060 g         1566 t
ORIGIN      
        1 actactgacc aacctccgtg ccggccggat cgatacggcc gaagtcgctc tgaaacagct
       61 cataaccctg cgcaaacaat acaacattgc caatctgtcg ccggttgcgg ctgccttcat
      121 cggctatttt gatcccggca ccaccgaatc gaccccgaag aagcgaagac agcttctcga
      181 gaccgcgatt gaacttgctc ccgactatcc tgccgtctat ttcaagctgg ccaaggttcg
      241 ccttttccag ggaattcaga acatcggccc ggcggccaca atcgccatgg acggtttcga
      301 acgaatgctg cgtttcccgc gcagcgcttc cattctggcg gccgagtcca gtttctatct
      361 gatcggcgtc ctgctgattc tatacatcat cctgggtctc ggcttcctga ttcgatactt
      421 ccccatgatc cgccacgaca ttggcgacat cctcccgaac gctgaaacaa cccacttcac
      481 cgcagccgaa attcgaaaat caagggggcc cctgcagagc ttcagtcggg gcctgtcgaa
      541 cggcctgctg acactgctgc tcctggtcat ctgtttcgcc ccgttcgtcc ttggggctgg
      601 attgcttctg accgccgcgc tctggatgct gctggtgttt ccatacggac gtctttccga
      661 gcgcatcatg atcgttgtca tgaccgtcgt ggcggcggcc ctgccgacca caggctcgct
      721 gaccgcgctt cccgggcatg tgaacggtac cgccggcacg ctgcgctggt cgtgcatcac
      781 cgaatactgc ccgcaggagt cccgacaggc tcttgaactt cttctggacg acggggttgg
      841 cgccactggc gtcggcgacc gggcacgaca cgaatcgacc atcgttgctg tcgcgctggg
      901 caggctccag ttcgcgattg ggcagccgga tcagatacca gccatccgaa gtttcatgga
      961 agcccgcaag gacagccttg aatctccgct gggccaggtt cttatgggca acgtcattgt
     1021 tcttgacgga cttcacaaat gccctgccgg gcggcacgac acggcagtaa tggcctcggc
     1081 gatgggctgg tacagccggg cgctcacggc ttcccccgac aacgaggccg cgaatcgtgg
     1141 aatggcagtt gcgtccggtc tggccggcga tcgcaagggc atggaactca gcctgcagcg
     1201 cgtccttgag attacgaagg aatccgatct tgatttcatg gcccaggtca ggacgctgac
     1261 ggcgtccagc gacgcatgcg atcacctggt ggaactggcg ggcgagcttc gccctccgcg
     1321 gccatcgtcc aatcacgaaa tttactttga cgggctttcc aatttcgagc ttctgacccg
     1381 gacgccgccg ctgctcccgt tcaagtcggt tcttctgggg gccgtcagcc cgcagatgat
     1441 tccgttaatc ctcgcggtaa tgcttgccgt gggcgtcgcg cttgtcttcg tcaatccaag
     1501 acggcgacgg gcggttcgtt gcccggcctg cggccacgtc atctgtcctt cctgcaacag
     1561 gcgggcttcc gggttcgagc attgcccgat atgccttttc gaccaggttc ggccggcatt
     1621 cctggatccc aaggacgtcg cggccatgtc cgccttccag catcgcggtc ttgccgccag
     1681 gtactggctg cccgcgatgt caatcgcggt tcccggctct gggcagatac tcggcggccg
     1741 cccgttccga gggcttacga tgatgcttct gcttggcctt ggcgcgatga tggtgataac
     1801 gccaagcgcg ccgatcatcg acagtttcgc atatattccg cagcaggccc gtggccttcc
     1861 actgctgccg ccgatcctgc tggtctcggc ttacctgtgg tcggtgattg acgtaatcat
     1921 cacaaggaaa agatgagcga cgatcttgaa atagcgatgt ccccgattcg cctggacgtc
     1981 gaaatgcatg acgaccagtc ggacgggtac gacgatgact cgtcaaagac gcgcgccttc
     2041 aatccgtttg aaaccggacc ggccggaagg cagaaatatc cgccgctgtt cgcggctctg
     2101 gccgtcatct accgcaggaa cctgacgggg gttcttaccg caaggcgtga tggcaaggac
     2161 gttctttcgg caacggtgga aaagggacga gtggtcagcg tggcccacca ggatcttacc
     2221 caggacacga ttcccgaact gctcctggac accggccttg tcagcatgaa gcacttcaag
     2281 gccgccgtcc gcaaggccga tcacaacaat acgtccatcg aggtcgaact ggtaaaggcc
     2341 aaccgcattt ccatgacaac cgcggcaaac ctgctcgagg ccaccctgac ggaaatcctt
     2401 ctgaaggtga tgggcgaacg cgacctggaa ttcacttttt tcaagtacct tccggccggt
     2461 cttcgaagga actgccacct gccgattccc ttcctgctga aggaagtgtc ccggcgcgca
     2521 gcggaactac cgggcatcat ggctcatttt ccggacgatt cggttggttt tgtcagaacg
     2581 tcccaactcg attcaaaccc ggaagaatac ctttgggagg atctcgacct cggttcggcg
     2641 gaaaagcaga tttatttctt tgtcgatgga catcgaacca tcgacgacct tcagaccgca
     2701 agcggccaat cgcgcttttc cgtcatgcgc gcacttgccg cccttatcga taccgggtac
     2761 gtcgtggcga ccgtggatcc ccccgatcgt tccggaacaa atcacacgtt ctggaatctg
     2821 gttcgcgccc tgagcctggt gctggtgttg accgggcttg ggttcctggt cggaacccgg
     2881 atttccccgg aacggcttcc gcacgtcagc acccgttacg ggttgagaac cgccgcctac
     2941 ggctcgacca tcatggattc gattcgggat cgggtgtccg gcgccatcct gtcttaccat
     3001 ctgctgacat caaaaaacga catcacggtt gataagttgc ttaacgatgg actgatcgac
     3061 gataacgacg cggttgtcgc cgaacagatc cttcaggccg ccgctgaaga gcaggcagaa
     3121 aaggaacggg ttcggaaact tctgatcatt cgtgagttgc aggaccggga agccccggca
     3181 caaaacagcg ccccggaaac ggcaaaggcc ccggagccgg ccgatgccgg gatgggagcg
     3241 gaaccgtccg atgatacaaa gtaatcaatc gatccaggtc gacatcgaat tttccgatat
     3301 caacgccttc atgtcgcttt gcggccaggg tgacgaaaat gtccgccgcc tggaagccct
     3361 gcttggcgtg gtcgtccgca tccggggaaa ccaggtcagc ctttacggcc agccggacgt
     3421 cgtcaaggtt gcccagggca tcctgaagca gctttacacg ctttgccagg cttcgcatga
     3481 cgttacccct gtcgatatag accgtgcagt ggccgtgatc gaggccgacc cggccgccga
     3541 ccttgccgac gtcttcgcgt ccggcgtttc aatcggcggc gtcccatcgc cggttcgccc
     3601 acggacgcca gcccagaaag gctacatccg agcgatgagg gacaacgatc tggtgatttc
     3661 ggtcgggccg gccggtaccg ggaagactta cctggcaatg gcgacggcgc ttggcttcct
     3721 tcaatcccac aaggtcaaat caatcgtcct gacgcgtccc gcggtcgagg ccggggaacg
     3781 ccttggcttc cttcccggcg acctggcgga aaaggtgaat ccgtacctgc ggccgctgta
     3841 cgatgccctt tacgacataa tggacaatga ccgcggccag cgccttatcg acaagggcgt
     3901 catcgaaatc gcgccactgg ccttcatgag gggccggacg ctttcgaatg cgttcatcat
     3961 cctggacgag gcccagaact gcaccgccga ccagatgaag atgttcctga cgcgcctggg
     4021 ctgcgggtcg cgcgccgtcg tgaccggcga tctgactcag acggatctgc cggccggcgt
     4081 tccgtcagga ctggagtatg cggtcaatgt gcttgccgaa gtaccgggga ttgcgatttc
     4141 caggtttacc gacgccgatg tgatgcgcca tccgcttgtc cgccggatta tccgtgcgtg
     4201 ggatggactc gacaaagggg gagccgatgg cggttcacgt ctataacaac cagaggaaag
     4261 tcaaaatcgt ccgtggctgc ttcgacgtcg cgtttgaaac ggccttgcgc attgccggcc
     4321 atgcggacga tgacgtcgaa gtcacgttcg tgtcgaatgc cgtgattgcc gaactgaacg
     4381 cacaatggcg cggcaagtcc ggcccgaccg actgcctctc gttcccattc gcgcctgacg
     4441 tgccgatgcc gcccgaagtc agggcgccgc ttggtgaaat cgtcatttcc ctggaacagg
     4501 cggctgctca gggaccgatt cacgtcaaat acgcggcggc cgggacgcat cccttcattt
     4561 ccgaggtcgt cttccttttc attcacagcc tgcttcacct gatcgggttc gaccacgaga
     4621 ccggaccgga agacgagcga atcatgttcg aaagacagga acaggtcttc gccgcggtca
     4681 tggcagagat ttcatagatt cgaggattgc ccgggcgaaa tttcctgtgc cgtcctcgta
     4741 cgggcgaaat ttcctgtgcc gtcctcgtac gggcggattt tcattcgccc gtacgcgggg
     4801 attacatccg cccgctgcgg ttcagcatga tgttcttgca tcgttccggc gggcggattt
     4861 tcattcgccc gtacgcgggg attacatccg ccctgatcaa cagattactt cgcgaaatcg
     4921 acggcccggg tttcccggat cacgcagact ttgacctggc ccgggtagga aagctgctcc
     4981 tcgatcttgc gggcgatgtc gcgggcaagg atggtggcgc cgtcatcgtc gatcctttcg
     5041 ttttcaacga tgacgcgaac ctcgcggccg gcctggatgg cataagacct gttcacgccg
     5101 gcgaacgagt tcgaaatctc ttccaggtcg ccaaggcgct tcaggtaatt ttccagactt
     5161 tcgcggcggg caccggggcg tgaacccgac accgcgtcgg cggcggccgt gatgtgggcc
     5221 agcagggtaa gcggcttttc ctcgttgtgg tgcgacgcta tggcatgaac gatctcggcg
     5281 ttttcaccga acttgcgggc aaaattggcg cccaccagcg cgtgcggtcc ctcgacctcg
     5341 tggtcaatcg ccttgccgat gtcatgcagc aatccggcgc ggcgggccat cttgaccgga
     5401 acgcccatct cggcagccat cagtccggca agcccgccaa cttcaaggct gtgctgaagg
     5461 acgttctggc catagctggt gcggtacttc atgcggccga tcaacttgac aagctccggg
     5521 tgcatcgtcc ccaggctgag ctggtacagc gcgtcctcac cggtttcctt gatgttctga
     5581 tcgacctcgc gggccgtctt ctcgaccagt tcctcgatcc ttgcgggatg aatccggccg
     5641 tcctgaacca gtttttccag cgcgagcctt gcgatctcgc ggcgaacggg attgaaaccg
     5701 gaaacgataa cggtatccgg tgaatcgtcg ataatcacgt cgatgccggt cgcctgttcg
     5761 aaagcacgta tgttccgacc ttcgcgcccg atgatgcgcc ccttcatctc ctcgctcggg
     5821 agttggacga cggaaacggt cctttcattg gcgaactcgg cggcgtaccg catcatggcc
     5881 acgccgacaa tcttctttgc gcgcttctcg gcctcttcct tggcctcttc ctcgatcatc
     5941 ttgatgcttt tcgcggccga gagcttgacg tcttccatca ggcgttccgt cagcatcgcc
     6001 ctggcctctt cctgactcag gccggcgacc gattccagga cggtccttgc ctcggcggtc
     6061 atcttttcgg cctcggccat ccgggccttc acatcggatt cccgctgaac ggtttccttt
     6121 tcgcgggaaa cgatttcgct ttcccgctgg accagcgtgt ccatcttacg atcaacggtt
     6181 tcctcgcgct tggccagccg ctcttccttg cggtcggcat cgctcttgac cttgttcagt
     6241 tccttttcaa gcgccttgac gcggatatct gcattccttg cctgtgacag agcctgctga
     6301 acctttgcct cccacttgac cgaactctcc tggatggcgg catccaggtt ttccttctcc
     6361 ttttcaagcc tcgacttgac ggtagcgcgt gtcgcaagga aagcgatcgc gccgccgccg
     6421 acaatgcctc caatggcagc cagaagtatc ccaaccataa cgaacctccc ggaacggcgg
     6481 cgcaacccgc gtccccgccg cacaaggtgc ggcatatcag ttaataactc caggctggga
     6541 tccgggggcc cgggaaatgt ggccccgtag agggatgagg actgctggcg tcgtaccgcg
     6601 cgctgtccgc cgggatcggc cgaatctggc cgcccgaacg tcttgtcgaa taccttactc
     6661 gcggaattta ttcaaaaaat ccgcgcaata caataaccag tcagatcaaa aaatatatgc
     6721 tcatccctcg cccggagggt tgtccccgac cagagtacgc ttcgactgaa gattccggct
     6781 ttcgtttaag gcataaagca gcttcgtcga ttgcgtacgt atattttcct tcaaatccgt
     6841 caaattcttc ttctcatgga acagttcctc ggccagatcc aaaaccgcca gaagcgccgt
     6901 cttgtgcggg gaaaaggatg gagccgccga agaaatcgaa tcgatgcggt tgttcgcggt
     6961 ctggctgatg aactcgagcg cgttgtcgtc cagatccgtc ttcagaacga atttctggcc
     7021 ctgaacggta atttcaacaa cccgcttttc caccggaacc tccgaaccga tcttcgaaag
     7081 tttaaggctt taacaaagat tgtcaatttt cgttgttact caagtgatac gggacagcgc
     7141 caggaccagt tgcggggacc gacctgtccc gggacattga tccggtggtc cgcggggtcg
     7201 cctggaagac ggccggccgc gtacagcagg tcgccaaggg cgtgcacggt aaac
//
LOCUS       DAOPPA010000108         7222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11471_length_7222_cov_3.633040, whole genome shotgun sequence.
ACCESSION   DAOPPA010000108 DAOPPA010000000
VERSION     DAOPPA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11471_length_7222_cov_3.633040"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKH54_04745"
     CDS             complement(<1..367)
                     /locus_tag="PKH54_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC99231.1"
                     /translation="MNAKRFQQILIGVLSFVAVVSTVLLFQTRSAMSSKPECPACPEQ
                     APAVQIPASRCDAGGPGANDALAVIHARTSVRAYKDQPVSLDQVEKMLKAGMAAPTAG
                     NRQPWYFVATTDRAVMDKLA"
     gene            527..1468
                     /locus_tag="PKH54_04750"
     CDS             527..1468
                     /locus_tag="PKH54_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOC99232.1"
                     /translation="MDSIAGFLNQWLSQSELEKIIESGIKVLMTLLLALVIFKLLRRA
                     LKRFEARLMAADEDLPDGSPSEHDKRIQTLVRLFRQAASIAIWVVVGLMILAEIGIDM
                     RPILAGAGIVGVAVGFGAQNLVRDIISGFFMIMENQIRVGDTVVINGTSGVVEQINMR
                     TVVLRDVGDVVHIFPNGMITTLANQTKGVSAYVFDVGVAYKEDTDKVTGVISGILDGI
                     VDDPEFGPKLNGKPEIFGVSRLGESSVEIRGRIRTLPGNQWVVGREFLKRVKHEFDRL
                     GIEIPFPHRTLMLADSAMSPVAEKILKDRVVEADSKD"
     gene            complement(1465..2673)
                     /locus_tag="PKH54_04755"
     CDS             complement(1465..2673)
                     /locus_tag="PKH54_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC99233.1"
                     /translation="MHPVPRHNINLDQKEMVAVITEILTGTVTAGPGDVAAFEREIAS
                     YHNVSQAVAVSSGRAAQCAVMKAMNLAPGSEVIVPSYSFFTVPGVVRALGHVPVFAPC
                     DPGTFAIDPARLESVITDRTSAVIVIHPFGQPAPVDQIVQICRRHGIHLIEDASQSIG
                     ATLRQRRVGSFGHASCMSLVHGKNLMTFGGGVVLTDDDRLASAIRSIVARNRPEKSIE
                     TRKKALAGLANWALTTKPGFAVGLLPPFWLLNFVSRERLDSIFVEKEAPFDPDSIRPL
                     SPFQARLGMMQLGHLDARNAVRRHNAERLIRGLSDIDWMTLPECMPGASCTFNAVPVR
                     VANPTDVQRELLMHGIDTRADYMTVYDDHEAWKQAGEIFYLPNHPGMNDADIDAVIRQ
                     VRAIARPRKA"
     gene            2733..3467
                     /locus_tag="PKH54_04760"
     CDS             2733..3467
                     /locus_tag="PKH54_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99234.1"
                     /translation="MIKSAAVGTMGAGSIILAEIRMEYELTISSDLAPTPLIAGFVWA
                     TGIDPVFSDELPGFLAPHVQKASWMGEDFWPRGIKARVRDMVRHGRYHPSGRSKPASE
                     FLLKAAVDGAFPAICGPVNINNAISLQSGLPISVFDADLTGPELLLRWGYPGESYVFN
                     RAGQSIDLQDLIVVCRRDGRGGWEPCGNPVKDSMATKVNERTTNVLAVIYAPNDYEPE
                     ELEKWCAMFADLLKSECHAESTGFRV"
     gene            complement(3708..5516)
                     /locus_tag="PKH54_04765"
     CDS             complement(3708..5516)
                     /locus_tag="PKH54_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99235.1"
                     /translation="MKRSLIVAVATTFLLAAAAGCGGGSGEYDVIADVPAGDIPADLA
                     MPDTTPADVQDPDAGADATEPDGATGELPDQEVSTNDATDDGLATDTVGDAFVKPDHL
                     PTALPVEFTRPQVGDPLSAEEVTEFTVRFTGFLKDTGYFKWVYDTSHGYDASTGKPDW
                     MVWWQDVDLIKEDGKLTFYHGDAGGAHNVMIPTTEILSAAQALYMQTGDEWAARVAEQ
                     YCKGITATMTGFLYDENDENIYVMARNIIGQNHEWTDINGHSKAVDFSHWYTTYSDWN
                     AERFEYASNPVFGDIWVTNLRSKDDVCHMFRTMALLPYLVQSEESGPVHEACSETLEY
                     MKGFARDIVDFDYNIRTKDSLGVAYLPGCENEKDLSSFVCFVGMDDQNECTARASAAL
                     VAYGDTRDVDCKSGFNSVYDQFSQITHCFNYHINWNFHMAAVANALVNWELDYAMGLM
                     AGLAKRMDSVLHPTDDEPGPKNCTDYEIEGAQMLAMTAPIGMPLTNEEVHHIHKIWDR
                     SIASYESWPRWDMWAETVPDGTYKYHGGDNSSRPKARDFVNEDGKTERLELDIQAIPM
                     LLEYCNSPLLNPSGAVLLDCDIVRDPARWGVMPDAT"
     gene            5719..>6673
                     /locus_tag="PKH54_04770"
     CDS             5719..>6673
                     /locus_tag="PKH54_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99236.1"
                     /translation="MSSSGYIRVCAIVAFAAGFSVTSPLAGAEEGGSLSVIVFPITVE
                     NAGVTGATLVSLQNFLEARLAAEGVLDVVSGDKARESLDDGEFDSCAARKCRILIGKQ
                     MSVDSFVETSISGVDGGCRISIALREVRTGSVLSSGSASTECAPARLKAALKGVARML
                     GDQAAGEGWVVDQSDIRDAGEGDADGTKKSEKKKVDEPPRPLAWVEIAGGSFDMGRAV
                     RGPDGDSLVSSRVAVPGFRMSETEVTNGQYLACVKAGACAAPHWDDLTCYMWIEGRWR
                     TGYLPNESRVNNFPVACIDRKQAESFAAWAGGRLPTEQEWEY"
     assembly_gap    6674..6683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6684..7015
                     /locus_tag="PKH54_04775"
     CDS             <6684..7015
                     /locus_tag="PKH54_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99237.1"
                     /translation="GGGLVRPFPWGDARPNCDLAVMTDPSTGCERDKPRKVCSVAVGN
                     STAGLCDMAGNVWEMVAAVDSGRQVLRGGSWRTGSERITTTGRGFIGCGYRDSDVGFR
                     VVQDIVK"
     gene            7015..>7222
                     /locus_tag="PKH54_04780"
     CDS             7015..>7222
                     /locus_tag="PKH54_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99238.1"
                     /translation="MFFPYGDTPNPRGFKPWMTRLLILANFAVYFLISSPLAGQQASA
                     ADPGWLAYLEFLAGHLGALTPEVIS"
BASE COUNT         1331 a         2053 c         2283 g         1545 t
ORIGIN      
        1 cggcaagctt gtccatgacg gctctgtcgg tggttgccac gaagtaccat ggctgtcggt
       61 ttccggccgt cggcgcggcc atgccggcct ttaacatctt ctcgacctga tccagtgaaa
      121 ccggctgatc cttgtatgcg cgcacgctgg ttcgtgcgtg gatcaccgcc agggcgtcat
      181 ttgccccggg accgcccgca tcgcaccttg atgccgggat ctgaaccgct ggagcctgct
      241 ccggacaggc cggacattct ggtttgcttg acatggcgct ccttgtctga aaaagaagca
      301 cggtcgaaac gaccgcgacg aaagacagca cgccaatcag aatctgctgg aatcgtttcg
      361 cgttcacggc tggaccccct ggtcaaacga gacagccaat tattatttat ttcgcgtccg
      421 cgtgataatg aaatcgccgc aggttccgaa ccctggggca cgaaacccgt gtttcgggat
      481 atattgtctc cttgcggacg ctttcaaatc gtggatggag gtgccgatgg acagcatcgc
      541 ggggttcctg aatcagtggt tgagccagtc ggaactggaa aagattatcg aatccgggat
      601 aaaggttctg atgaccctgt tgcttgccct tgtcatcttc aagttgctga gacgggcatt
      661 gaagcggttc gaggcgagat tgatggcggc ggatgaagac ctgcccgacg gctccccgag
      721 cgagcacgac aagaggattc agacgctggt gagactgttc cggcaggccg cgtcgatcgc
      781 gatatgggtt gtggttggcc tgatgattct ggccgaaatc ggcatcgaca tgcgaccgat
      841 cctggccggc gccgggatcg tcggcgtcgc cgtcggtttt ggcgcgcaga acctggtgcg
      901 ggatatcatt tccgggtttt tcatgatcat ggaaaaccag atacgcgtgg gcgataccgt
      961 cgtgatcaac ggcacgtccg gggtggttga gcagatcaac atgcggaccg tcgtgctgcg
     1021 agatgtcggc gacgttgttc acatattccc gaacggaatg atcacgaccc ttgccaacca
     1081 gacgaagggt gtttcggcct atgtctttga tgtcggcgtc gcttacaagg aagacacgga
     1141 caaggtaacc ggcgtgattt ccgggattct tgacggaatc gtggatgatc ccgagttcgg
     1201 gccgaagctg aacgggaagc ccgagatatt cggcgtgtcc cggcttggcg agtcgtccgt
     1261 tgaaatccgt ggccgcatcc ggactctgcc cggcaatcag tgggtggtcg gtcgcgagtt
     1321 cctgaagcgg gtcaaacacg agttcgacag gcttggcatt gagattcctt tcccgcatcg
     1381 aaccctgatg ctggcggatt ccgccatgag cccggttgcc gaaaagatcc ttaaggatcg
     1441 cgtcgtcgag gcggattcga aggactaggc cttcctgggc ctggcgatcg cgcgtacctg
     1501 acggatgaca gcgtcgatat ccgcgtcgtt catgcctgga tggtttggaa gatagaagat
     1561 ttccccggcc tgtttccatg cttcatggtc gtcataaacc gtcatgtaat cggcgcgggt
     1621 atcgattccg tgcatcagga gctcgcgctg gacgtcggtc gggttcgcca cgcggacggg
     1681 aaccgcgttg aaggtgcacg acgcccccgg catgcactcg ggcaacgtca tccagtcgat
     1741 gtcggacagg ccccggatca gtcgttcggc attgtggcgg cggacggcgt tcctggcgtc
     1801 cagatggccg agctgcatca ttcccagcct tgcctggaac ggtgaaagcg ggcgaatcga
     1861 gtctgggtcg aacggggctt ccttttcgac gaaaatggag tcaagtcttt cccggcttac
     1921 gaagttcagc agccagaaag ggggaagaag cccgacggcg aagcccggct ttgtcgtcag
     1981 agcccagttt gccaggccgg ccaacgcctt tttcctggtc tcgatggatt tttcggggcg
     2041 attccttgca actatcgacc tgatagccga cgcgagcctg tcgtcatcgg tcaggacgac
     2101 gccgccaccg aaagtcatca ggttcttgcc gtgcacaagg ctcatgcagg acgcatggcc
     2161 gaacgaaccg acgcgtcttt ggcgcagagt cgcgccgatc gactgggatg cgtcctcgat
     2221 aaggtgaatt ccgtggcgac ggcagatctg gacgatttga tcgactggcg ccggctggcc
     2281 gaacgggtga atgacgatga ccgccgatgt cctgtcggtg atgaccgatt ccagccttgc
     2341 gggatcaatc gcgaaggttc ccgggtcgca cggcgcgaac accggtacgt ggccaagcgc
     2401 acgaaccacg ccgggaaccg tgaagaagga atacgatgga acgatcactt ccgaacctgg
     2461 ggcaaggttc atcgccttca tcacggcgca ctgggcggcg cggcccgagg atacggcaac
     2521 ggcctgggaa acattgtgat atgaagcaat ttcccgttcg aaggccgcga catcaccggg
     2581 cccggcagtc accgttccag taaggatctc ggtgatgacg gcaaccattt ccttctgatc
     2641 caggtttatg ttgtgacgtg gaacggggtg catggccagc ctccggatcg cggattttga
     2701 gcctatccta tcagaccgga cccgatttca atgtgataaa atcggccgcg gtcggcacga
     2761 tgggtgcggg cagcataatc cttgcggaaa tcaggatgga atacgaactc actatctcga
     2821 gcgatcttgc cccgacgccg ttgattgccg gtttcgtctg ggccacgggc atcgacccgg
     2881 tgttttccga tgaacttccc ggttttttgg cgccgcatgt ccagaaggca tcctggatgg
     2941 gtgaggattt ctggccacgg ggcatcaagg cccgcgtgcg cgacatggtt cgtcatggtc
     3001 ggtaccatcc ttcggggcgc agcaagccgg ccagcgaatt tctgctgaag gcggccgtcg
     3061 atggggcgtt tccggccatc tgcgggccgg tcaacatcaa caatgcgata agccttcagt
     3121 ccgggcttcc gatttcggtt ttcgacgcgg atctgaccgg cccggaactg ctgcttcgat
     3181 ggggatatcc cggcgaatca tacgtcttca accgcgcagg ccagtcaatc gacctgcagg
     3241 atctgattgt cgtgtgccgg cgcgacggtc gtggcggctg ggagccgtgc ggaaatccgg
     3301 tgaaggactc gatggcgacc aaggtgaatg aacggacgac gaatgtgctg gcggtcatct
     3361 atgcgccgaa cgattatgaa cccgaagaac tcgagaagtg gtgcgccatg ttcgccgacc
     3421 tgctgaagtc cgagtgtcac gccgaatcca ccggattcag ggtctgaagt acaccagtct
     3481 ccctgtacgg gcggattaca tccgcccgct gcgatcctgc cagttgccca tccgcgccgt
     3541 ctgccgcctc attgtacggg cggattacat ccgcccgctg cggtccagca aaatgctgtc
     3601 catcgcgtcc gtcggttgcc cgccccgcga tccgtacggg cgaatgaaaa ttcgcccgcc
     3661 tgcgtgatga ttcgccggcc agcgtgtcga tgaagtcgtc gggtggatca ggtggcgtcg
     3721 ggcatgactc cccagcgggc cgggtcgcgg acgatgtcgc aatcgagcag tacggcgccg
     3781 gacgggttca gcagcgggga gttgcagtat tccagcagca tcggaatggc ctggatatca
     3841 agctccagac gttctgtctt gccgtcttcg ttgacgaaat cgcgcgcctt ggggcgcgat
     3901 gagttgtcgc cgccgtggta cttgtaagtt ccgtccggaa cggtctcggc ccacatgtcc
     3961 cagcgcggcc agctttcata gcttgcgatg ctgcgatccc agatcttgtg aatgtggtgg
     4021 acttcctcgt tggtcagcgg cattccgatt ggtgctgtca tggccagcat ctgggcaccc
     4081 tcgatctcgt aatcggtgca gtttttcggg ccgggctcgt catcggtcgg atgcaggacg
     4141 gagtccatgc gcttcgccag tccggccatc aggcccattg cataatcgag ttcccagttg
     4201 accagggcgt tggccacagc cgccatgtgg aagttccagt tgatatgata attgaaacag
     4261 tgggttatct ggctgaactg atcatagacc gagttgaatc cggacttgca gtcaacgtcc
     4321 ctggtgtccc catacgcgac aagcgcggcc gaggcccgtg cagtgcattc gttctggtcg
     4381 tccatcccga cgaagcacac gaacgacgaa agatcctttt cattctcgca gccgggcagg
     4441 tatgcgacgc caagcgaatc cttcgtcctg atgttgtaat cgaaatcgac gatgtcccgg
     4501 gcgaagccct tcatgtattc aagagtttcc gaacaggctt cgtggaccgg gccggactcc
     4561 tcggactgga ccaggtacgg aagcagggcc atcgtcctga acatgtggca tacgtcgtcc
     4621 ttgctgcgca ggttggtcac ccagatgtcg ccgaagaccg ggttggacgc gtattcaaaa
     4681 cgttcggcat tccagtcgga atacgtcgtg taccaatgtg agaaatcaac tgccttggaa
     4741 tggccgttga tgtctgtcca ttcgtggttc tggccgatga tatttcgcgc catgacatag
     4801 atgttttcgt cgttttcgtc gtacaggaag ccggtcatcg tcgcggtgat ccccttgcag
     4861 tactgctcgg ccacgcgggc cgcccattcg tcgccagtct gcatgtacag ggcctgggcc
     4921 gcgctgagga tttctgtggt cggtatcatg acgttgtggg ccccgcccgc gtcgccatga
     4981 tagaaagtca gcttgccatc ttccttgata agatcgacgt cctgccacca gaccatccag
     5041 tccggcttgc cggttgatgc gtcgtatccg tgggaggtgt cgtacaccca cttgaagtat
     5101 ccggtgtcct tcaggaatcc ggtgaaccga acggtaaact cggtcacttc ctcagccgaa
     5161 agcggatccc cgacctgcgg ccgggtgaat tccaccggaa gtgcggtcgg aagatggtcg
     5221 ggtttgacga aggcatcccc gacggtgtcc gtcgccaagc cgtcgtcggt cgcgtcgttc
     5281 gttgatactt cctggtcggg aagctcgccg gtcgctccgt cgggctcggt cgcgtcggcg
     5341 ccggcgtccg gatcctggac gtcggccggt gtcgtgtccg gcatcgccag atcggccggg
     5401 atgtcgcccg ccgggacgtc ggcgatgaca tcgtattcac cagacccgcc gccacatccg
     5461 gcggcggccg caagcaggaa ggttgttgca actgcaacga tcaggctccg tttcatgatt
     5521 gggctcctgg ccgacaaaga cacgacattt tacatgcaag gtcgaattgg tcagcataaa
     5581 ttgtccaacc ccgcgacccg gacgcggaat ttgacggttc gccggactta caaaacgggc
     5641 ctgcggatgt tataacaagc gttggcttcg attccccggt tccccgtggc cgccgccggc
     5701 ggttgaagag gtgtgatttt gtcatccagc ggttacatcc gtgtttgcgc cattgttgcc
     5761 ttcgcggctg gattttccgt cacgtcgcct ttggctgggg cggaagaggg cggcagcctc
     5821 tccgtaatag ttttcccgat aaccgtcgag aatgccggcg ttaccggcgc aaccctggtg
     5881 tcgcttcaga atttcctgga agccaggctc gcggccgagg gcgtactgga cgtcgtgtcc
     5941 ggggacaagg cgcgtgaaag ccttgatgat ggcgagttcg attcgtgtgc ggccagaaag
     6001 tgccggatcc tgattggtaa gcagatgtcc gtcgattcgt tcgtcgagac gtcgatcagt
     6061 ggcgtggatg gcggctgccg gatttcgatt gcccttcgtg aagtcaggac aggttcggtg
     6121 ctttcttccg gctcggcatc gaccgaatgc gctccggcca ggttgaaggc cgcgctgaag
     6181 ggtgttgcca ggatgcttgg cgaccaggcc gccggcgagg gatgggtcgt tgaccagtcg
     6241 gacatccggg acgccgggga aggcgacgcc gatggcacga agaagtccga aaagaagaag
     6301 gtcgatgaac cgccgcgccc gctggcctgg gtcgagatcg ccggcggttc tttcgacatg
     6361 gggcgcgcgg tgcgcggtcc ggacggtgat tccctggttt cttcacgggt tgccgtgccg
     6421 ggcttcagga tgtccgaaac cgaggtcacg aacgggcagt acctggcatg cgtgaaggcc
     6481 ggtgcctgcg ccgcgccgca ctgggacgac ctgacctgtt acatgtggat tgaaggtcgc
     6541 tggcggaccg ggtaccttcc aaatgagtcc agggtcaaca attttcccgt cgcatgcatc
     6601 gacaggaagc aggccgaatc attcgcggca tgggccggcg gccgtctgcc gaccgagcag
     6661 gaatgggaat acgnnnnnnn nnngtggcgg cggtctggtg cgtccgttcc cgtggggcga
     6721 tgcccgtccg aactgcgacc ttgccgtcat gaccgaccca tcgacaggct gcgagcggga
     6781 caagccgcgc aaggtctgtt cggtcgccgt cggcaattcc acggccggtc tttgcgacat
     6841 ggccggcaac gtctgggaaa tggtcgccgc ggtcgattcc ggcaggcagg tgcttcgcgg
     6901 cggaagctgg cgcaccggct cggaacggat aacgacgacc ggccgtggtt tcatcggttg
     6961 cggttaccgt gattccgacg tcggtttccg ggtcgtccag gacatcgtca agtaatgttt
     7021 tttccatacg gtgacacccc gaatccccga ggcttcaagc cctggatgac caggctgctg
     7081 atccttgcca acttcgcggt atatttcctt atttcaagcc cgttggcggg acagcaggcc
     7141 agtgcggcgg atccgggctg gcttgcctat ctggagtttc ttgccggtca cctgggcgcc
     7201 ctgaccccgg aagtgatttc cg
//
LOCUS       DAOPPA010000109         7182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11547_length_7182_cov_4.479164, whole genome shotgun sequence.
ACCESSION   DAOPPA010000109 DAOPPA010000000
VERSION     DAOPPA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11547_length_7182_cov_4.479164"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..657
                     /locus_tag="PKH54_04785"
     CDS             124..657
                     /locus_tag="PKH54_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99239.1"
                     /translation="MDVGGDDAGARVIGSLDDVLDPDEIEVAAVLNFNRPFTADPDQA
                     VAMIRGIEGNARMRVTGVIANTHLMQFTKPETVRSGYENACECARRLSVKVLAVCVPS
                     VLEGEFSGDEFESKLFPISRVIRTTFAADTDSAGGDSGVPEQPPAPGFAPAVEGRYGV
                     RKVGPLFVPAAMPDEEG"
     gene            659..892
                     /locus_tag="PKH54_04790"
     CDS             659..892
                     /locus_tag="PKH54_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC99240.1"
                     /translation="MPRIDVEKERCKGCELCVKACPQGVLAIGTEMNGKGYFFVKRVE
                     PTKCLGCRLCCITCPDVALSLNVNGTMYHYFKY"
     gene            943..2025
                     /locus_tag="PKH54_04795"
     CDS             943..2025
                     /locus_tag="PKH54_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOC99241.1"
                     /translation="MARKLMKGCEAIGEAAIKANCKLFFGYPITPQNEIPEYMSRRLP
                     QVGGTFVQAESEVAASNMLYGAAGAGERVFTSSSSPGISLMQEAVSYMAGSELPVVIV
                     NIMRAGPGLGGILPSQADYFQSTKGGGHGDYRLLVLAPSSVQEAVDLMQDAFDLADHY
                     RNPVMVIADGLIGQMMEPVEFKEDRQPCRSLPSKDWATTGWKGEGRPRAIVNSLFLDP
                     NLLERHNEKLTAKYAEMARNEVRVAEYFTDEDYDLLVVAYGSVARVCLSAIRQLRAEG
                     KRVALVRPISLFPFPTEVIERAANKAGRVLVVELSTGQMLEDVRMAVGKDFPVAFHGR
                     TGGNLVTPDEAREKILAQLAAREASR"
     gene            2022..2819
                     /locus_tag="PKH54_04800"
     CDS             2022..2819
                     /locus_tag="PKH54_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC99242.1"
                     /translation="MKDKLAFGYPEGLTTGGTHYCPGCTHGIIHRLIAEVIDELGVRE
                     RTVGVSPVGCAVLAYNYFSTDYQEASHGRAPAVATGIKRARPDLMVYTYQGDGDLASI
                     GMAEIVHAANRGEKFTVVFVNNAIYGMTGGQMAPTTMPGQVTSTSPRGRELDLAGHPI
                     RMVELISTLHTPAFVARGTVIRPLQAVQTKELLRRAFQYQKEGKCFSMVEILSTCPTN
                     WGMPPAEALNWLEKTMMPYYPLGVFKTPEEGVDRAPLPLCGLQDGGK"
     gene            2819..3367
                     /locus_tag="PKH54_04805"
     CDS             2819..3367
                     /locus_tag="PKH54_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC99243.1"
                     /translation="MQHEMIFAGFGGQGVLLIGKMIAYAGLNSGKEVSWLPSYGPEMR
                     GGTANCTVVVADEPVGSPVVASPWAVVAMNLPSLDKFEPTVAPGGVLVINTSLINRPA
                     VRTDVRVVNVAANEICNELGNPKGANMVTVGCFVGATEVVPVQQMEDLIRETFAAKPK
                     LIDVNVECFRRGLEVGRAQKAV"
     gene            3370..3750
                     /locus_tag="PKH54_04810"
     CDS             3370..3750
                     /locus_tag="PKH54_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC99244.1"
                     /translation="MTEKITVYQVDHALCIGCHRCFKVCTYQAVSGNPKEKHTIDPAR
                     CMACGACFDCPKKAISKVEVEASVRTVEIARVRCECCGALTITTAQADWGSRRKGVNA
                     EAFKLCDECKRRRMVGQARQLMGC"
     gene            3772..4215
                     /locus_tag="PKH54_04815"
     CDS             3772..4215
                     /locus_tag="PKH54_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC99245.1"
                     /translation="MDRIFRVVPEKCVACGKCELACAFAHGQDGVPGVSRIRIYRRGP
                     EAGVPIICLQCDSAACAAACSVGAIKWNVETGALEVDRDKCVGCMMCVAACPFGNIYM
                     EPGLDKAAKCDLCGGKPQCVAFCPTGAIAYVPTEAGVKYPCESAV"
     gene            4240..5694
                     /locus_tag="PKH54_04820"
     CDS             4240..5694
                     /locus_tag="PKH54_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99246.1"
                     /translation="MRCHRVSQFSRYSALFPSASFCMVLTAMSLISGVARATDATEPL
                     LDSLVEANFKDDYGAAGAALRQLVEFQRAAGIEPSGKLAVVQVNETLAFADKMDARIS
                     RYIEDGFPVLAAMELAKFRDVLLFEPAGAARARDAAAKVQEAGRTLCGELRATAAGAA
                     RAVILAEAVCAAFGDGRPAVGQGGGQAAGLGPGQGLSPVAAPVGIINVDSRVKGFLQS
                     VPIDKSLNAAAAARGLLSNGAEGVLSCVIDGTSVVSKSSLPGTLAFEYGVQVPYSEMQ
                     EYCEWKEDASTKIVEQDGRKKKVTETTRTCIKKQRPVDLVRVDKHVRQVSGTNWKRSA
                     VWQIRASFDDGSGCSGQKSFDGQRVDNDFVHDKSDPAIGLKPDPLEFPGEDAVAADLA
                     AQVATWLVGVYGDCQKARFCGPAGSGRFGGDGVAGENTGTAGAATGAEPPSLDSVLRC
                     AGIPGGDRGQVDAAIVAKFGVTLNELLAFCGLSP"
     gene            5920..6831
                     /locus_tag="PKH54_04825"
     CDS             5920..6831
                     /locus_tag="PKH54_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC99247.1"
                     /translation="MSLTFKRYLSLLVDKPPARPGDGINVTWLGNAGVHVTDGITGFL
                     IDPFVSRYGMLRVALGLGMPPMQDVIAKWAATFDPALVGAVIASHSHYDHALDAPFFA
                     RLFDAPLVGTESTANIGRGARLSDDFIKVVGHADTMEIGAFKITFLESVHGPALFNRV
                     PYPGVITSPLVSPAGARGYRMGGVFGIVIQHPAGTLVHHGSAGYDRDMYAGHHADVVL
                     LGIAGRAGTSTYLKNVVDALGARTVIPIHFDNMFHPVSTSFRFMPAVHFDEFLDVCLQ
                     TRPNMNVSTLPVGRPVGLLINGSLNPP"
     gene            complement(6978..>7182)
                     /locus_tag="PKH54_04830"
     CDS             complement(6978..>7182)
                     /locus_tag="PKH54_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99248.1"
                     /translation="LPWDLYPKFVSKKTGRGLRIHLDSLWAQVHRQQPGREENHHAWA
                     MDSLSSAYVVCDLYLKGQIIKNN"
BASE COUNT         1369 a         1946 c         2355 g         1512 t
ORIGIN      
        1 agccatactg gcctcatgga gccgttttgt ggatgaaagt tcctacacac tttctacgcc
       61 gatctgccga tcatgctccc ccgtgttcgc ggtttgtgca ggcagcacga ccggcgggta
      121 gtaatggacg ttggcggcga cgatgcgggg gccagggtca tcggatccct tgacgacgtg
      181 ctggatcccg atgaaatcga ggtcgcggca gtcctgaatt tcaacagacc gttcaccgcc
      241 gatcccgatc aggccgtggc gatgattcgt gggattgaag gaaacgccag gatgcgggtc
      301 accggcgtga tagccaacac gcacctgatg cagtttacca agcctgaaac ggttcgatcc
      361 ggatacgaaa atgcgtgcga gtgtgctcgt cgtcttagtg taaaagtatt ggccgtctgc
      421 gtgccttccg tgctagaagg cgaattcagc ggcgacgaat tcgaatcgaa gctcttcccg
      481 atttcccggg tgatccgcac gacatttgcg gcggataccg actccgcggg tggggattcg
      541 ggtgttccag aacagccgcc ggcgccggga ttcgcgcctg ccgttgaagg tcgttacggg
      601 gtccggaagg tcggcccctt gtttgttccg gctgccatgc cggacgagga aggttgagat
      661 gcccaggatt gatgtcgaaa aggagcgctg caagggctgt gagctctgcg tgaaggcttg
      721 cccccagggg gttctggcca tcggcacgga gatgaacggc aagggttact tttttgttaa
      781 gcgggtggaa ccgacgaagt gcctgggatg caggctttgc tgcatcacat gcccggacgt
      841 ggcgttgtcc ctgaatgtca acggcacgat gtaccactat ttcaagtact gaccggcgac
      901 aggcggtctt tgcaggtccc gcgggaccat tgaggtttgg ccatggccag aaaacttatg
      961 aagggttgcg aggcgatcgg cgaagcagca atcaaggcga actgcaagct gttcttcggc
     1021 tatccgatca ctccgcaaaa cgagattccc gagtatatgt cgcgcaggct gccgcaggtg
     1081 ggcggaactt tcgttcaggc ggaaagcgag gtcgctgcat ccaatatgct ttacggcgcc
     1141 gcgggcgcgg gcgagcgcgt gttcacgtca tcttcgtcgc ccggcatcag cctgatgcag
     1201 gaagcggttt cgtacatggc cggttccgaa ttgcctgtcg tcatcgttaa catcatgcgc
     1261 gccggcccgg gtcttggcgg aatccttccg tcccaggccg actatttcca gtcaaccaag
     1321 ggcggtggtc acggtgatta ccgccttctg gtgcttgcgc cttcttcggt tcaggaagca
     1381 gtcgatctga tgcaggatgc gttcgacctg gccgatcatt accggaatcc ggttatggtc
     1441 attgccgacg gcctgatcgg tcagatgatg gagcccgtcg agttcaagga agaccgccag
     1501 ccatgccggt cgcttccgtc caaggattgg gcgaccacgg gctggaaggg cgagggtcgt
     1561 ccgcgggcca tcgtcaattc gctgttcctc gatccgaacc tgctggaaag gcacaacgag
     1621 aagctgaccg cgaagtatgc cgagatggca aggaacgagg tcagggtggc cgagtacttc
     1681 accgatgaag attacgatct gctggtcgtt gcgtacggct cggtggcgcg cgtctgcctt
     1741 tccgcgattc ggcagcttcg ggccgaaggc aagcgtgttg ccctggtgcg tccgatttca
     1801 cttttcccgt tcccgacgga agtgattgaa agggctgcaa acaaggcagg tcgcgtgctt
     1861 gtcgtcgagc tttcgaccgg ccagatgctg gaggatgtcc ggatggcggt cggaaaggac
     1921 tttccggtcg cgttccatgg aaggacgggt gggaacctgg ttaccccgga cgaggctcgt
     1981 gaaaagattc tggcgcagct cgccgccagg gaggcttcca gatgaaggac aaacttgcat
     2041 tcggatatcc ggaaggattg accacgggtg ggacacacta ctgcccggga tgtacccacg
     2101 gcatcatcca caggctgatt gccgaagtaa tcgacgaact gggcgtccgc gaaaggaccg
     2161 tcggcgtttc tcccgtcggc tgtgccgtcc ttgcgtacaa ttatttttcg acagactacc
     2221 aggaagccag ccatggacgc gccccggcgg tcgccaccgg catcaagagg gcccgtccgg
     2281 atctgatggt ttacacctat cagggtgacg gagacctggc atcgatcggg atggccgaaa
     2341 tcgtccatgc cgcgaatcgt ggcgaaaagt tcaccgtcgt gttcgtcaac aacgcgattt
     2401 acggcatgac cggcggccag atggctccga cgacgatgcc gggccaggtg acctcgacgt
     2461 ctccacgcgg ccgtgaactg gatctcgccg gccatccgat tcggatggtc gagcttattt
     2521 cgacgctgca taccccggcc ttcgtcgcgc gcggcaccgt gatcaggcct ttgcaggccg
     2581 ttcagaccaa ggaactgctg cgcagggcat tccagtacca gaaggaagga aagtgcttca
     2641 gcatggtcga aatcctttcg acttgcccga ccaactgggg catgccgccg gccgaggcgt
     2701 tgaactggct ggagaagacg atgatgccgt actacccgct gggagtcttc aagacaccgg
     2761 aggaaggcgt ggatcgcgcc ccgctgccgc tttgcgggtt gcaggacgga ggtaagtgat
     2821 gcagcacgag atgatttttg ccggtttcgg cggccagggt gtcctgctta tcggaaagat
     2881 gattgcgtac gccggcttga acagtggcaa ggaagtaagc tggcttcctt cttacggccc
     2941 cgagatgcgc ggcggcacgg cgaactgcac ggtcgtcgtg gcggatgaac cggtcgggtc
     3001 gcctgttgtg gccagcccgt gggcggttgt cgcgatgaac ctgccgtcac tggacaagtt
     3061 cgagccaacg gtggccccgg ggggcgttct ggtcatcaat acgtcgctta tcaatcggcc
     3121 ggcggttcgc accgatgtcc gggtggtcaa cgtcgcggca aatgaaatct gcaacgagct
     3181 tggcaacccg aagggcgcga acatggtcac ggtcgggtgt ttcgtcggcg cgacggaagt
     3241 ggttccggtt cagcagatgg aagacctgat tcgcgagacg ttcgcggcca agccaaagct
     3301 gattgacgtt aacgtggaat gcttccggcg cggactggaa gtcggccggg cacagaaggc
     3361 ggtgtaatca tgactgaaaa aataaccgta tatcaggtcg atcacgcgct gtgcatcggc
     3421 tgtcacagat gtttcaaggt ctgcacgtac caggcggtaa gcggcaaccc gaaagaaaag
     3481 cacaccattg atcccgccag gtgcatggct tgcggcgcct gcttcgattg tccgaagaag
     3541 gcgataagca aggtcgaggt cgaagcgtcg gtaaggacgg tcgaaatcgc ccgtgtccgc
     3601 tgtgaatgct gcggtgcctt gaccatcacc acggcccagg ccgactgggg cagcaggcgc
     3661 aagggcgtca atgccgaggc cttcaagttg tgcgacgagt gcaagcgtcg caggatggtc
     3721 gggcaggcaa ggcagttgat gggttgctga ggcaaccttt gaggttatgt aatggacagg
     3781 atattcaggg ttgttccgga gaaatgcgtg gcgtgcggca agtgcgagct cgcatgtgcc
     3841 ttcgcccatg gacaggacgg tgttccgggc gtcagcagga tcaggattta caggcgtggt
     3901 ccggaggcgg gcgtgccaat catatgcctg cagtgcgatt cagccgcttg cgctgccgct
     3961 tgcagtgtcg gggccattaa gtggaacgtt gaaacgggcg cgctggaggt cgatcgcgac
     4021 aagtgtgtcg gatgcatgat gtgcgtggcg gcgtgcccgt ttggaaacat ttatatggaa
     4081 ccaggcctcg acaaggcggc caagtgcgac ctttgcggcg gtaagccgca gtgtgtcgca
     4141 ttctgtccga ccggggccat cgcgtacgtc ccgacggaag ccggggtgaa gtatccctgc
     4201 gagtccgccg tgtgacgtgt ccgcccaggg ggggtacgga tgcgctgcca tcgtgtttca
     4261 cagttttccc ggtactcggc gctttttcca tcggcatctt tctgcatggt cctgacggcg
     4321 atgtcattga tttcgggggt ggcaagggcc accgacgcca ccgagcccct gcttgacagt
     4381 cttgtcgaag caaatttcaa ggatgactac ggtgcggccg gcgcagccct gcggcaattg
     4441 gtggagttcc agcgcgcggc gggaatcgag ccgtcgggca agctggcggt tgtccaggtc
     4501 aacgagacgc ttgcctttgc cgacaagatg gacgcgagga tttccaggta tatcgaggac
     4561 ggatttccgg tgctggcggc gatggaactg gcgaagttca gggacgtcct gctctttgaa
     4621 ccggcgggtg ccgccagggc cagggatgcc gccgccaagg ttcaggaggc tggaaggacg
     4681 ctgtgcggtg aactcagggc cactgcggcc ggtgcagcgc gagcggtgat cctggccgag
     4741 gctgtgtgtg ccgcgtttgg cgatggccgg ccggcggtcg ggcagggcgg gggacaggcg
     4801 gctggcctgg ggcccggcca gggattgtcg ccggtggccg cccctgtcgg cattatcaat
     4861 gtcgattcaa gggttaaggg tttcctgcag tcggttccaa tcgataagag cctgaatgcg
     4921 gccgcggcgg cgcgcgggct tctttccaat ggcgccgagg gtgtgctttc gtgcgtgatt
     4981 gacggtacat cggtggtgtc gaaaagcagc ctacccggta ctcttgcatt cgagtacggc
     5041 gtgcaggttc cgtactcgga aatgcaggaa tactgcgaat ggaaggaaga tgcatcgacg
     5101 aagattgttg aacaggacgg caggaagaag aaggtcaccg aaaccacgcg cacctgtatc
     5161 aagaaacagc ggccggtcga tctggtaagg gtcgataagc acgttcgaca ggtcagcgga
     5221 accaactgga agcgaagcgc cgtatggcag atccgggcga gctttgacga cggttcgggc
     5281 tgcagcggcc agaagtcgtt cgacgggcag cgagtcgaca acgatttcgt tcacgataaa
     5341 agcgatcccg cgattgggct gaaaccggat cctctcgaat tccccggcga ggacgccgtt
     5401 gctgccgatc ttgcggcaca ggttgcgacg tggttggtcg gtgtctatgg tgactgtcag
     5461 aaggccaggt tttgcggtcc ggccgggagt gggcgtttcg ggggcgatgg tgtcgcgggc
     5521 gagaataccg ggacggcagg tgccgcgact ggtgccgagc cgccgtccct ggattcggtt
     5581 ctgcgttgtg ccgggatacc aggcggtgat cgaggccagg tcgatgccgc gattgtggcg
     5641 aaatttgggg tgaccctgaa cgaattgctg gcgttctgcg gcctttcccc gtaggtgcga
     5701 atgaccattc gacaggctca gggcatgcaa ttgaacccgt aagggcgaat gaaaattcgc
     5761 ccgttgcctg gaatttcgct gcctgtctgt gatatttggt cgcccgtgtt cttgtatggg
     5821 cggattacat ccgcccgccg cgaaccgtaa gggcgaatga aaattcgccc gttggcagga
     5881 atttcgcccg tggctccggc cgttcttccg tgaggtaata tgtccttaac attcaagcgc
     5941 tacctgtcct tgctggttga caagcctccc gcccggccgg gggatgggat taacgtaacc
     6001 tggcttggta atgccggggt tcacgtaaca gatggcatca ccggcttcct gattgatccc
     6061 tttgtcagtc gttacggtat gttacgggtg gcgcttggtc ttggaatgcc gccgatgcag
     6121 gatgtcatcg ccaaatgggc ggccactttc gaccccgcgc tggtgggggc ggtcattgcc
     6181 agccacagcc attacgatca tgcccttgac gccccgttct ttgcccgtct gtttgacgca
     6241 ccgcttgtcg gcaccgaaag cacggccaat atcgggcgcg gcgcccgtct ttccgacgat
     6301 ttcataaagg tggtcggcca tgctgacacg atggaaatcg gggctttcaa gattactttc
     6361 cttgaaagtg tccacgggcc ggccctgttc aaccgcgtcc cctatccggg cgtcatcacc
     6421 agtccgctcg tctcgcctgc cggtgcccgg ggctacagga tgggcggggt tttcggcatt
     6481 gtcatccagc accctgccgg tactcttgtg caccacggaa gcgccgggta tgaccgggat
     6541 atgtatgcgg gacatcacgc ggacgtcgtg ctgcttggga tagccggtcg ggccgggaca
     6601 tcgacatatc ttaagaacgt ggttgacgcc ctgggcgcca ggacggtcat cccgattcat
     6661 ttcgacaaca tgttccaccc agtatcgacc agtttcagat tcatgccggc ggttcatttt
     6721 gacgagttcc ttgacgtctg cctgcagacg cgtcccaaca tgaatgtttc gacccttccg
     6781 gttggccggc cggtcggcct ccttatcaat ggttcgttga atccgccttg acgggtgcga
     6841 ggcatgaaat cacgccggcc ggaatgattc agcgcgatcc gacgagtccg aaacagagca
     6901 cgtttgaata tgtgataatg gcgttcgcca gtaaacgtac gccagttgaa cgctgggcgc
     6961 ggcgctccgg tctccgttca gttgttcttg attatttggc ctttcaggta taaatcacaa
     7021 accacatagg cgctcgatag actatccatc gcccaggcgt ggtggttttc ttcgcgacct
     7081 ggctgttgtc ggtgaacttg agcccatagg gagtccagat gaattcttaa accgcgcccg
     7141 gttttcttgg acacgaactt gggataaaga tcccagggga gg
//
LOCUS       DAOPPA010000110         7112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11684_length_7112_cov_3.648009, whole genome shotgun sequence.
ACCESSION   DAOPPA010000110 DAOPPA010000000
VERSION     DAOPPA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11684_length_7112_cov_3.648009"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4690)
                     /locus_tag="PKH54_04835"
     CDS             complement(<1..4690)
                     /locus_tag="PKH54_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99249.1"
                     /translation="MSDKHLLGLALAVCTALTVACGGGNERPDSGSPDVTNDVPGDVI
                     RPDQGGTDSNIRDTGNEVGTDVPDADEEVAGDVVADTKDVQTGCKIDDDCPDDPDNLC
                     QIGKCLDGGTCGFGPRSCNDGFDCTADGCSTENGGCYHIYLRATPCTECVNDSDCGSE
                     DPCDQNACRDVPNPQTSPEYYDEFPVPDSGKVCFRKPCAGCDDKNPCTNDSCTPQGVV
                     INQERSCSDYNQCTTDSCSVNSGECVHEPVTPCVVCTAGDHAACNTAATNPDPKCLIG
                     YCKPVLFDGGTHECSATQSCPNGAECDLEAGKCMAYVCDYQANTCDDANACTADFCDS
                     SLGCQHRCICGSCENVTQLPEESVECDDGSRCTLDSCIEAAGTGCTGKTRVCQYEELD
                     CDDGLENTVDSCDPLSGCLHTTIITCTTDEECAADNNKCTIREYCNGVTGLCVSSPLA
                     CDDGDPCTFDECSPSTGCVFTTIPGCYTDCTGSEGVCNDSNACTIDSCEVNPADTLFK
                     ICRNRTINCDDADPCTKDWCDVRLGCQHLETQCACDSNDDCVSDKGCLQGRCNTNTGL
                     CEEFETSCSDGDSCTLDRCDHGTGQCLHLPDPNAPLDRCIECEPSDPNSDALCNANST
                     DPCKTGFCNDQTGYCNFQAIDCSDGDSCTTDTCDAVKGCLHTKITDCKHCPNGQDWEC
                     TTSNKCLRGKCDKLADPKGLCIWEPVACDDGNPCHSGECNPATGGCIWTDLPVDDGNA
                     CTIDSCNENTGMSHVEKICSDGLRCTADTCDPVTGNCVFTPKNCDDQNPCTTDYCSPS
                     SGVCVWVPVDCDDGNQCTEDTCVDGLGCSHWINPWPPCNGEGCVRDNQCNDNNPCTTD
                     SCNKTRVPSSCDFIPKDCEDNNPCTLDLCNPVTGKCEHRPTMCDDFDPCTIDTCSPTG
                     SCAHTTAWCSDGDRCTTDSCDSDGGGCIFLPYRNTDPEGPAACKTFTCANDNDCNVLG
                     NNCVKGKCNTGTGVCFYDIVVPCDDGLACTVDQCNPVTGKCQHNPLPCDDGNLCTTDT
                     CDEQIGGCVFRFKNCDDSNACTDDVCDSTSGSCTHRIQVCDDKNPCTRDSCDPITGFC
                     RYDDKNCSDNDKCTKDYCDVTTGLCVSVPEACDDNNLCTVDVCDAATGLCRHPTKDCD
                     DGNLCTTDVCSTFDGGCYHDEKECVPTTVGFVGECAPATGLCVYREKDCDDGNACTAD
                     RIDSWGNCRNETIDCNDGNVCTLDTCDPATGCRHVDTTSSCKDTNPCTVDSCDPMVGC
                     MFKYVANPPACPACVSDTTCYDQLNLNKCVVSACDIPNGVCKYFPTVSCNDNNPCTID
                     TCDGTTGLCAHAQKDCNDNIPCTIDSCDTANGTCTNVARTCDDGNPCTIDSCDFLTGD
                     CVNVENPCNDNDPCTYDTCLPETGCTHSPEVYDDGNKCTVDSCDPRTGDSRHVPIVCN
                     DKNPCTDDFCNPATGECRTSATDCDDGNACTTDSCDPVLGCVHTGKVCDDGNCCTRDR
                     CDSITGVCVFSPESCDDNNPCTLGTCDPLSCGCTYRTVNCDDGDPCTWDNCSPAAGGC
                     RFNPVDCDDGL"
     gene            4958..5416
                     /locus_tag="PKH54_04840"
     CDS             4958..5416
                     /locus_tag="PKH54_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99250.1"
                     /translation="MKSIAVSIALSLFACLMPFSVHAQSAGEEVAPELFELGEIQVKF
                     TNTDYPSVRLDGESWEETEYTQDGRRVSLHRVNRMSQHVVKAIPNVPGFEEAEVVIEP
                     ADWKLVKVNKLDRAWKVEKSLTFKKKPVEPKKPAPPPAEDEAGAVAPEVP"
     gene            complement(5637..5915)
                     /locus_tag="PKH54_04845"
     CDS             complement(5637..5915)
                     /locus_tag="PKH54_04845"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOC99251.1"
                     /translation="MGTTVETDIARIVAAANEKTFSGAPAYLIVEKCVGCANAIQHEG
                     KSFCKVYKDPAAKWMFGQCNLGTHIVRKAEEQKKVNPLKASKKAMKGA"
     gene            complement(5984..6151)
                     /locus_tag="PKH54_04850"
     CDS             complement(5984..6151)
                     /locus_tag="PKH54_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99252.1"
                     /translation="MPSSTKKTKLITKRKATKSGARRKREIRHDYNVKVQEVGRMLGL
                     DTPDALANKTE"
     gene            complement(6257..6937)
                     /locus_tag="PKH54_04855"
     CDS             complement(6257..6937)
                     /locus_tag="PKH54_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOC99253.1"
                     /translation="MFYFDPLYFVFLGPALLLSIWAQARVKLAYSKYSKVPSSTGMTG
                     ARIARAMLDSEGVQGVSIEMVPGKLSDHYDPSKKILRLSDGVYNGTSVAAAGIAAHEV
                     GHAIQHARKYSPLVIRTAMVPLAQLGSNLSWILMLVGFLISVTELVWAGIILFGAAVL
                     FSIVTLPVEFNASARAKEALPRLGLVAASDSRGVNAVLNAAAMTYVAAAATAVLQLVY
                     FVLRANRN"
BASE COUNT         1233 a         2014 c         2277 g         1588 t
ORIGIN      
        1 caagtccgtc gtcgcagtca accgggttga accggcatcc gccggcggcc ggcgagcagt
       61 tgtcccacgt gcaggggtcg ccgtcgtcgc agttgacggt cctgtaggtg catccgcagg
      121 aaagcgggtc gcaggtgccg agcgtgcacg ggttgttgtc gtcgcacgat tcgggcgaga
      181 acacgcaaac gccggtaatc gagtcgcacc ggtcgcgggt gcagcagttg ccgtcgtcgc
      241 agaccttccc ggtgtggacg cagccgagca ccggatcgca tgagtcggtc gtgcaggcat
      301 tgccgtcgtc gcagtcggtc gcgcttgtgc ggcattcacc cgtcgccggg ttgcagaaat
      361 cgtcggtgca gggattcttg tcgttgcaca ctatcggtac atggcgcgaa tcgccggtgc
      421 gcgggtcgca ggaatcgacc gtgcacttgt tgccgtcgtc atagacttcc ggcgagtggg
      481 tgcagccggt ttccggcagg catgtgtcgt acgtgcaggg atcgttgtcg ttgcacggat
      541 tttcgacgtt cacgcaatcc ccggtcagga agtcgcagga atcgattgtg cacgggttgc
      601 cgtcgtcaca tgtccttgcg acgttcgtgc acgttccgtt ggccgtatcg cacgagtcga
      661 tggtgcacgg gatgttgtcg ttgcagtcct tctgggcgtg cgcgcacagg ccggtggtgc
      721 cgtcgcaggt atcaatcgtg cacgggttgt tgtcgttgca tgaaacggtc gggaagtact
      781 tgcatacgcc gttcgggatg tcgcatgccg agaccacgca cttgttcagg ttgagctggt
      841 cgtagcatgt cgtatccgaa acgcaggccg ggcaggccgg cggattcgcg acgtacttga
      901 acatgcagcc caccatcggg tcgcatgaat cgacggtgca gggattggtg tccttgcatg
      961 acgaagtcgt atcgacgtgg cggcatccgg tggccgggtc gcaggtgtcc agcgtgcaga
     1021 cgtttccgtc gttgcagtct atcgtttcgt tacggcagtt gccccacgaa tcgatcctgt
     1081 cggcagtgca cgcgtttccg tcgtcgcagt ccttttcgcg atagacgcac aggccggtcg
     1141 caggcgcgca ttcgccgaca aatccgaccg ttgtcgggac gcattccttt tcgtcgtggt
     1201 agcagccgcc gtcgaaggtc gagcagacgt cggtggtgca caggtttccg tcgtcgcagt
     1261 cctttgtcgg gtgcctgcag aggccggtcg ccgcatcaca gacatcgacg gtgcacaggt
     1321 tgttgtcgtc gcaagcttcc ggcaccgaga cgcacagccc cgtcgtcacg tcgcagtaat
     1381 cctttgtgca cttgtcgttg tcgctgcagt tcttgtcgtc gtacctgcag aatcccgtaa
     1441 tcggatcgca ggagtcgcgg gtgcacgggt tcttgtcgtc gcagacctgg atcctgtggg
     1501 tgcacgaacc ggaagtcgag tcgcacacgt cgtcggtgca ggcattgctg tcgtcgcaat
     1561 tcttgaagcg gaacacgcat ccgccgatct gttcgtcgca ggtgtcggtg gtgcacaggt
     1621 tgccgtcgtc gcacggcagc gggttgtgct ggcacttgcc ggtcacgggg ttgcactgat
     1681 cgacggtgca ggccaggccg tcgtcgcacg ggacgacgat gtcatagaag cagacgccgg
     1741 ttccggtgtt gcattttccc ttgacgcagt tgttgccaag gacgttgcag tcattgtcgt
     1801 tggcgcaggt gaaggtcttg caagctgccg gtccctcggg atcggtgttc cggtagggca
     1861 ggaagatgca tccgccaccg tctgagtcgc aggaatcggt cgtgcagcga tcgccgtccg
     1921 agcaccaggc cgtcgtgtgg gcgcacgagc cggtcggcga acatgtgtcg atcgtgcatg
     1981 gatcgaagtc gtcgcacatg gtcgggcggt gttcgcactt tccggtcacc gggttgcaca
     2041 gatccagcgt gcagggattg ttgtcctcgc agtccttcgg gatgaagtcg caggaactgg
     2101 gaaccctggt cttgttgcac gagtcggtcg tgcacgggtt gttgtcgttg cactggttgt
     2161 cccttacgca gccttcgccg ttgcaagggg gccaggggtt aatccagtgc gagcacccca
     2221 ggccgtcgac acaggtgtct tcggtgcact ggtttccatc gtcgcagtca accggcaccc
     2281 atacgcagac gccgctgctg gggctgcagt aatcggtggt gcaggggttc tggtcgtcgc
     2341 agttcttcgg ggtgaagacg cagttgccgg ttaccggatc gcacgtgtcg gccgtacagc
     2401 gcaggccgtc cgagcagatc ttttcaacgt ggctcatgcc ggtgttttca ttgcaactgt
     2461 cgatggtgca tgcgttgccg tcgtcaactg gcaggtcggt ccagatacac ccaccggttg
     2521 ccgggttgca ttcgcccgag tggcacgggt tgccgtcgtc gcacgcaacc ggctcccaga
     2581 tgcacaggcc cttcggatcg gccagtttgt cgcatttgcc gcgcaggcac ttgttggacg
     2641 tggtgcattc ccagtcctgt ccgttcgggc agtgcttgca gtcggtaatc ttcgtgtgca
     2701 gacaaccctt cacggcgtcg catgtgtccg tcgtgcacga atcaccgtcg ctgcagtcga
     2761 ttgcctggaa attgcagtat cccgtctggt cgttgcagaa gccggtcttg cacgggtcgg
     2821 tgctgtttgc gttgcacagc gcgtcggaat tcggatccga cggttcgcat tcgatgcagc
     2881 gatccagcgg ggcattcggg tctggcaggt gcaggcactg gccagtgccg tgatcgcacc
     2941 tgtcgagggt gcaactgtcg ccgtcgctgc aacttgtttc gaattcctcg caaaggccgg
     3001 tgttcgtgtt gcagcgcccc tgcaggcagc ccttgtcgct gacgcagtcg tcgttgctgt
     3061 cgcacgcgca ctgcgtttcg agatgctggc agcccaggcg tacgtcgcac cagtccttgg
     3121 tgcacgggtc tgcatcgtcg cagttgattg tcctgttacg gcagattttg aaaagagtgt
     3181 ccgcgggatt gacttcgcaa ctgtcgatgg tgcaggcatt gctgtcgttg cagacgcctt
     3241 cggaaccggt gcagtcggtg tagcagccgg ggatcgtcgt aaagacgcat ccggtcgagg
     3301 gcgaacactc gtcgaacgtg cacggatcgc cgtcgtcgca cgcaagcggt gacgagacgc
     3361 acagtccggt taccccgttg cagtattcgc gaatggtgca tttgttgttg tcggcggcgc
     3421 attcctcgtc ggtcgtgcag gtgataatcg tggtgtgcag gcatcccgaa agcgggtcgc
     3481 aactgtcgac agtgttttca aggccgtcgt cgcagtcgag ctcttcgtac tgacagaccc
     3541 tggtttttcc ggtgcagccc gttccggcgg cctcgatgca ggaatcgagc gtgcagcggc
     3601 ttccatcgtc gcactcgacg ctttcttccg gaagctgggt cacgttttca caggaaccgc
     3661 agatgcagcg gtgctggcat cccaggctgg agtcacagaa gtccgcggtg cacgcgttgg
     3721 cgtcgtcgca cgtgttggcc tggtaatcgc agacgtaggc catgcacttc ccggcctcga
     3781 gatcgcattc cgcgccgttc gggcaggact gcgtggcact gcattcgtgc gttccgccgt
     3841 cgaaaagcac gggcttgcag tatccgatca ggcacttcgg atcggggttt gtggcggcgg
     3901 tgttgcaggc ggcatggtca ccggccgtgc acaccacgca tggtgtaacc ggttcgtgaa
     3961 cgcactcgcc gctgttgacg ctgcatgaat ccgtcgtgca ctgattgtag tccgaacatg
     4021 aacgttcctg gttgattacg acgccctggg gggtgcagga gtcgttggtg cacgggttct
     4081 tgtcatcgca tccggcgcac ggcttgcgga agcagacctt gccggaatcc ggaaccggga
     4141 attcgtcgta atattcggga gaagtctggg ggttcgggac gtcgcggcac gcgttttgat
     4201 cgcagggatc ctcgctgccg caatccgaat cgttgacgca ttccgtgcac ggcgttgcac
     4261 gcaggtagat gtgatagcag ccgccgtttt cggtcgagca gccgtccgcc gtgcagtcga
     4321 agccgtcgtt gcagctcctg gggccgaagc cgcaggttcc gccgtcgagg catttgccaa
     4381 tctgacaaag gttgtcgggg tcgtccgggc agtcatcgtc aatcttgcag ccagtctgga
     4441 cgtccttcgt gtcggcaacg acatcgccgg cgacttcctc gtcggcgtcg ggcacgtcgg
     4501 ttccgacttc gttaccggtg tcgcggatgt ttgaatcggt tccgccctga tcgggacgga
     4561 tgacgtcgcc aggaacgtcg ttggttacat ccggtgaccc ggagtccggt ctttcgttgc
     4621 cgccgccgca ggcaaccgtc agagcggtgc acaccgcaag agcaagaccg agaagatgct
     4681 tgtcagacat ggcgtttctc ctgaacctga agaatccaat ggatgcataa gaaagccccc
     4741 gtcgggactt aatccgacgt acgcctaccc ccgggcatac gcagcttgat aagtttatca
     4801 gaattttccc gggaataaaa agggttattc gcaggttaat cgatgctttc tacagccgcg
     4861 gggctttgaa tcggacggga cttgtattta taatcaacgg tgccgtcgat gaatcgacca
     4921 ggcgtggtcg tttcgtcctt ttcccggagg tggaatcatg aagtcaattg ccgtttcgat
     4981 tgccctgtct ttgtttgcat gcctgatgcc attttcggtt catgcgcagt cagcgggcga
     5041 ggaggttgct cccgaactgt tcgaactggg cgaaatccag gtcaagttca ctaacaccga
     5101 ctatccttcg gtaaggctcg acggcgagtc ctgggaagaa acggagtaca cccaggatgg
     5161 tcgcagggta tcccttcatc gggtcaacag gatgtcccag cacgtcgtga aggcgattcc
     5221 caatgttccc ggtttcgagg aggccgaggt cgtgatagag ccggccgact ggaagcttgt
     5281 gaaggtcaac aagctggatc gggcgtggaa agtcgagaag tcgctgacat tcaagaagaa
     5341 gcctgtcgag ccgaaaaagc cggccccgcc tcccgccgag gacgaagccg gtgccgtcgc
     5401 cccagaagtt ccgtgaagtt ttcttgggcc gtcttgctgc ccccagccca gttaaaatct
     5461 tcctgccgtg aaggcgccat gcttctgttg aatcattccg ggatcgcaag gggttgcaag
     5521 tagccaggaa tcgggctgtc gcgtcaggtt taaggggtgc ggagacagtt gtattgtgct
     5581 gctggcgaca agccggcacg cgcggcgacg ggctgctggg ttgaaacaat ccggggtcag
     5641 gcgcccttca tggccttctt ggaagccttc agcgggttga ccttcttctg ctcttccgcc
     5701 ttgcggacga tgtgcgtgcc aaggttgcac tggccgaaca tccacttggc ggccggatcc
     5761 ttgtacacct tgcagaagga cttgccttca tgctggatgg cgttggcgca accgacgcat
     5821 ttttcgacaa tcaggtacgc gggggcgccg ctgaaggtct tttcgttggc ggcagcgacg
     5881 attcgtgcaa tgtctgtttc cacggtggtg cccatttcag tctccgtggc gggtcggcgt
     5941 ggccgatccc gcggcgtatt acaggccccg gttcgaaccg ggactactcg gtcttgttcg
     6001 caagcgcgtc cggcgtatcc agtcccagca tccggcccac ttcctggacc ttgacgttgt
     6061 aatcgtggcg gatttccctc ttgcgacggg ctccggactt tgtggccttg cgtttcgtaa
     6121 tcagcttcgt cttcttcgta cttgaaggca tttccctggc ctcctgacaa caagggtgca
     6181 caattatatc gacaagattt ttcatgtcaa ctcgataagt gaatcgtcga taggggcgga
     6241 cgtcgggtgc cggcggtcag ttcctgttgg cgcgcagaac aaaatacacc agttgcagga
     6301 ccgcggtcgc tgcggccgcg acataggtca tggcggcggc gttcaggacg gcgttgactc
     6361 cacgggagtc ggatgccgcg accagtccga gtcgtggaag ggcctccttc gcacgggccg
     6421 acgcgttgaa ctcgacaggc agcgtcacga tcgaaaacag gacggccgcc ccgaacagga
     6481 ttattccggc ccagaccagt tcagtgacgc tgataaggaa tcccacaagc atcaggatcc
     6541 acgaaaggtt cgagccaagc tgggcaaggg gtaccatcgc ggttcgaatc accagcggcg
     6601 agtacttcct ggcgtgctgg atggcatggc cgacttcgtg cgcggcgata ccggccgctg
     6661 caaccgacgt cccgttatag acgccgtccg acaaccgcag gatcttcttt gacgggtcgt
     6721 agtgatccga gagcttcccc ggaaccattt cgattgaaac accctgtacg ccttccgagt
     6781 ccagcatggc cctggcgatg cgggccccgg tcattccggt cgacgaaggc actttcgaat
     6841 atttcgagta agcaagtttc accctggcct gggcccagat gctcagcagc agggccggcc
     6901 cgaggaagac gaaatacaag gggtcaaagt aaaacatctc ttttcaccat gaatcgaaaa
     6961 agccggaagg aaagatgggt atcccggagg ttgttgtcaa cgaggatttg cgtcaggcct
     7021 tgttgcgctc ggccattgcc tcgcggcggg aaaggcggat cttgccgttc ttcggatcga
     7081 caccgatgca cttgaccagg atttcgtcgc ct
//
LOCUS       DAOPPA010000111         7068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11781_length_7068_cov_3.829745, whole genome shotgun sequence.
ACCESSION   DAOPPA010000111 DAOPPA010000000
VERSION     DAOPPA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11781_length_7068_cov_3.829745"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   54..1413
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=66..86
                     /rpt_unit_seq="gcggggtgcgtggattgaaac"
     gene            1574..2020
                     /locus_tag="PKH54_04860"
     CDS             1574..2020
                     /locus_tag="PKH54_04860"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOC99254.1"
                     /translation="MHAHDGRSELFKVAIKRRADARRLYEGGRPHYSGAMYLAGYSVE
                     CKMKAIVMEIMGVVTLRGLAEKLRMDDGDIYTHGLDAILELSPLRDQLRRSDVWLDFV
                     SQVNRWRPSWRYDPRERSGAEAKRYLDAVDRVYNWLDAGRYGGHRG"
     gene            2023..2277
                     /locus_tag="PKH54_04865"
     CDS             2023..2277
                     /locus_tag="PKH54_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99255.1"
                     /translation="MRSRDEIKKAIEVAIRKEFPTDTVDISDGYGSNIHVMVVSRRFD
                     SMDDAIQRDLLWTLIRSAGLDDQEEALISLALTISPSIIK"
     gene            complement(2772..6971)
                     /locus_tag="PKH54_04870"
     CDS             complement(2772..6971)
                     /locus_tag="PKH54_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99256.1"
                     /translation="MNRAFRVLSVSIMVAFMATTLVACGGGGDGEPDVIYDVTLPDSQ
                     MDSMQDVPPDTTPPDVRPDEGTDVVCTCTVADDCCDGCNVINEGGACAMEGAGNCKTP
                     VCQSGACVAVDVVCAPDGKCLFDNGTCNLETGACEFDLSGALPNGTSCEAQPGIDGSG
                     ACILGVCQPFGECDFRAYDQVAGMPCNFDSECASGLCMADGWGWDHFCTQRCETAACP
                     DGLRCTKNADNEYFCMRIGTDNYPGDGTIELFKPCNRDEDCAGGLCLGFDTTRFCTMN
                     CESEAGGTKDSAKCGECGACEDGGTDNGFQFEFYCVPEAEGREGAPCQSPLDCNNHFC
                     YEGYCSNQCFIFGEDLDSCPDGFECVLNAYNGDVAICVADTSLNKPFGAECMHDYECS
                     VGTCRDVAGTTRCITGCDDCTDGECRQVGWIRIDTMMELWKDGAEAAVATDDNAGEGN
                     LSRISYYVQEAGTYFIGVMGVDDVITGPYYLDVRLGEQAMAETIVDEVEANDDKAGAE
                     AKTAPFRVKATMEAAGQDWFKFEVAFPEAATSVRVLAEAVQFEDLACVPKTVDGGAGY
                     GEACVWEWQCADGFDCLNGVCNKVCDIDSDCPDGICFAYSDTDKRCVPAELVGGKDIQ
                     DTCEYAWECDEMCLADGYLGEYYCSKACTTELDCPHGMGCVDKVCNKGFPTPNFPYGY
                     CRMNADCESYVCTDGMCTETCAADEDCEGAEAIVPEGPMDLCASCATNADCNDGTEDF
                     FNEAYCVQVSETEMFCTPQCTFDEGSCPVGTRCYSLDGFTKVCAPVSFTCQAGGVGCQ
                     ETAGLCVRPYINDGDACRFDEECIGGKCANGICRSETCDGDLECDCELLACNGGYCEY
                     YAAEGRVMEVEPNNDVDTAQVIQSIPMLVMAAFNPVGMTQYDNDIFKFAVTGGMAYSF
                     ITGPSCGVETDPAMLLRDGDGEYIDGYAIADSATSYFPAIFGYVPAENGFVYVEIVQD
                     PWVDGFLRQPYVLEVSSFLPVTGDNCSDDVAMPIALSALAMNNVTDSTASPTVVGDTA
                     GPDLVRWVDVPGVTDPGNFSVYLVQAEPNEPADNLSMWAFTDCDDPAGTAVTWSNFNV
                     GKEGTEGLTLVNTTADPVRYFIAMNVSRFGAGPVNLTTLMTMGDTPFNYQGESLDSTL
                     FVEIEDTPVMQFMNLAYPWTNDVAPTGGLCADKDMSGKDIVYSVKVPDGKFLKVEMKE
                     RFTAANMYILDAQDTNNCVAFGPGPLYYMPEWITMPPVAVDHDVYIVVEPNGHPGGAF
                     SMELSIMDVGECAGPCDPATAAVECADTDNICYCDQNTKFWNKFDCKADCLAQDYTIG
                     GECHLYSTGDSDGQSGCMCHYDCSIDWLPGAMCDNMSYTNCTCSSENPCGWANDDYCD
                     DFCMNEYDDHFDDTGDCTPAP"
BASE COUNT         1317 a         2073 c         2237 g         1441 t
ORIGIN      
        1 cggggtgcgt ggattgaaac gctctggcta tgctgtacac cgatccggat tccgtcgcac
       61 cccgcgcggg gtgcgtggat tgaaactggg acggcgccgt cgaagtcagc gacaccgaca
      121 gtcgcacccc gcgcggggtg cgtggattga aactcgtgca cgcgcgggaa ttgtcgcctg
      181 acgatgtcgc accccgcgcg gggtgcgtgg attgaaacag gtgccggtcg ttgagcacgt
      241 catcgaatac cgcgtcgcac cccgcgcggg gtgcgtggat tgaaacatag gtaacgggcg
      301 aaaccgaggg cacccactcg agtcgcaccc cgcgcggggt gcgtggattg aaacaagatg
      361 accgctcgtg gtttgctggc gtcggccgtc gcaccccgcg cggggtgcgt ggattgaaac
      421 tactacaaca agtaagctag gttcctactt tgcagtcgca ccccgcgcgg ggtgcgtgga
      481 ttgaaacttt tttgaacttc ataatgccac ctatacttgc gcgtcgcacc ccgcgcgggg
      541 tgcgtggatt gaaacgccct caccgttgaa gtagatgcag cagagtcagt cgcaccccgc
      601 gcggggtgcg tggattgaaa cttttcagcg cccgcagttg cggatcttac gccccgtcgc
      661 accccgcgcg gggtgcgtgg attgaaacct gcccgtgaat cgccgccagg tcagcgaccg
      721 acgtcgcacc ccgcgcgggg tgcgtggatt gaaacgttgt tcttcggcat tttcatgtcc
      781 tcgtcaaagg tcgcaccccg cgcggggtgc gtggattgaa acagaaggtc cccgagaagg
      841 tgtccggggc tctcgagtcg caccccgcgc ggggtgcgtg gattgaaacc gatctcctct
      901 gcatggctgt cgcctgtgag cccgtcgcac cccgcgcggg gtgcgtggat tgaaactaca
      961 ccatcggcaa catcaagtcg ctgggcgggg tgcgtggatt gaaacaacat gccggcgctg
     1021 gccgccctgc tggaagcggg gtgcgtggat tgaaacacga cgtcggatac tttggtgccc
     1081 ctgaactgga gtcgcacccc gcgcgggtgc gtggattgaa accggtacgt cgccggaaac
     1141 tgctgaatga ccgctgtgtc gcaccccgcg cgggtgcgtg gattgaaact ggtctcgacg
     1201 gtgaccacgc ccataacctt ggacgtcgca ccccgcgcgg gtgcgtggat tgaaacagcc
     1261 cgtcctgata ctgcataccg ttgtgctttt gtcgcacccc gcgcggggtg cgtggattga
     1321 aacgcgtcgt agtccacgtt ggccgcgggg tgcgtggatt gaaactgact gttccggttg
     1381 ctgctggtaa cctcgcgggg tgcgtggatt gaaacagggg cttccgggac cgccggcgct
     1441 gacggggtgc gtggattgaa accacatgct tggatgaaac gcttttcgtc gattcccgat
     1501 aaatgttgcc gtatccccgg gagttattaa gtcgcagcct ctttgtcagg cagtgaattc
     1561 gcgaggtaac ccgatgcatg cacacgatgg tcgcagcgaa ttgttcaagg ttgcaatcaa
     1621 gcgtagagcc gatgcccgaa ggttgtacga aggtggccga ccccactatt cgggagcgat
     1681 gtacctggcg ggatactccg tggaatgcaa gatgaaggcg atagtgatgg aaattatggg
     1741 cgtcgtgacg ctgagggggc ttgcagaaaa attgcgtatg gacgacggtg atatctatac
     1801 gcacggattg gatgcgattt tggagctcag tccacttcgt gatcagcttc gtcgaagcga
     1861 cgtgtggttg gattttgttt cccaggtcaa tcgctggagg ccttcctggc gatacgatcc
     1921 cagggagcga tccggggcgg aggcgaaaag ataccttgat gccgtggatc gtgtttacaa
     1981 ttggctggac gccgggcgtt acggcggaca cagggggtaa tgatgcgcag cagggatgaa
     2041 atcaagaagg cgatagaggt cgcgattcga aaggagttcc caacggatac ggttgatatc
     2101 tctgacggct acggcagcaa tatccacgtc atggtcgttt cccgacggtt tgacagcatg
     2161 gacgatgcaa ttcagcggga tttgctgtgg accttgattc gttctgccgg tcttgatgac
     2221 caggaagagg cgctgatcag tctggcgttg accataagcc catcgataat caagtgagtt
     2281 tagttctgaa tcccgtcacc ggtaattgat ccatccgcca ttgttcccgg tggggtttcc
     2341 cgcgctattc gggggcggcc cagtggctat ttgtccgatt ccctggtgat atcggcaacg
     2401 tcaatcgcgc atcggaaacc aatgtcggtc acggggacat attccttatc tgtgggcgaa
     2461 tttcctgtcc tggcgggcga attttcattc gcccatacag ggaaggcggc aggcggggca
     2521 acttgcagag tcgcagcggg cggatcgggc cggaccataa gtgtccggtg aggcctctcg
     2581 aaccggacac ttcatggccg gccacaggca cgactgcgtc actcgtgggg cgtacggcag
     2641 ctcctcgtcc cccgagcgaa gcgaagggcc ccacgggcca gcgaagcgag ccatgccaac
     2701 gggcgcgatg ggaatttttg cagaaccgca gcgggcggat gtaatccgcc cctacagggc
     2761 cggatggtcg attacggggc cggggtgcag tcgccggtgt cgtcgaagtg gtcgtcgtat
     2821 tcgttcatgc agaagtcgtc gcagtagtcg tcgttggccc agccgcaggg attttccgac
     2881 gagcatgtgc agttcgtgta cgacatgttg tcgcacatgg cgccgggcag ccagtcaatc
     2941 gagcagtcgt aatggcacat gcagccggac tggccgtcgc tgtcgccggt cgagtacagg
     3001 tggcattcgc cgccgatggt gtaatcctgg gcaaggcagt cggccttgca gtcgaacttg
     3061 ttccagaact tcgtgttctg gtcgcagtaa cagatgttgt ccgtatcggc gcattccacc
     3121 gcggccgtgg ccgggtcgca cggaccggcg cattcgccca cgtccatgat cgacagttcc
     3181 atcgagaacg caccaccggg gtgtccgttc ggttcgacga cgatatacac gtcgtggtca
     3241 acggcaaccg gcggcatcgt gatccattcc ggcatgtaat acagcgggcc ggggccgaac
     3301 gccacgcagt tgttggtatc ctgggcgtcc aggatataca tgttcgcggc ggtgaacctt
     3361 tccttcatct cgaccttcag gaacttgccg tccggaacct tgaccgaata cacgatatcc
     3421 ttgccggaca tgtccttgtc ggcgcacagg ccaccggttg gcgcgacgtc gttcgtccag
     3481 gggtacgcca ggttcatgaa ctgcatgacc ggggtatcct cgatttcgac gaacagcgtc
     3541 gaatccaggc tttcaccctg gtaattgaac ggcgtgtcgc ccatcgtcat cagtgttgtc
     3601 aggttgacgg ggccggcgcc gaacctggaa acgttcatcg cgatgaagta acgaaccggg
     3661 tcggccgtcg tgttgaccag ggtaagaccc tcggttcctt ccttgccgac gttgaagttc
     3721 gaccaggtaa ccgcggtgcc ggctggatcg tcgcaatcgg tgaacgccca catggaaagg
     3781 ttgtcggcag gctcgttcgg ctctgcctga accagataga cgctgaaatt gccgggatcg
     3841 gtcacgccgg ggacatcgac ccagcgcaca aggtcgggtc cggccgtgtc gccgacgacg
     3901 gtcggtgaag ccgtcgagtc ggttacgttg ttcatcgcca gggccgacag ggcgatcggc
     3961 atcgccacgt cgtccgaaca gttgtcgcca gtgaccggca ggaaactgga gacttccagg
     4021 acgtacggct ggcgaaggaa tccgtcaacc cacggatcct ggacgatttc cacatagacg
     4081 aagccgttct cggccggcac gtatccgaag atcgccggga agtagctggt ggccgaatcc
     4141 gcgattgcgt aaccgtcgat gtattcgccg tcgccgtcac gcagaagcat ggccgggtcg
     4201 gtctcgaccc cgcaggacgg gccggtgatg aatgagtacg ccatgccgcc ggttacggcg
     4261 aacttgaaga tgtcgttgtc gtactgggtc atgccgacag gattgaacgc ggccatcacc
     4321 agcatcggaa tggactgaat gacctgggcc gtatcgacgt cgttgttcgg ctcgacttcc
     4381 atgacgcgac cctcggccgc atagtattcg cagtatccgc cattgcaggc cagaagttcg
     4441 caatcgcatt caaggtcgcc gtcgcatgtt tcggatcggc agatgccgtt tgcgcacttg
     4501 ccgccgatgc attcttcgtc aaagcggcac gcgtcgccgt cgttgatgta aggacgcacg
     4561 cacagtccgg ccgtttcctg gcatccgacg ccgccggcct ggcaggtgaa cgacacgggg
     4621 gcgcaaactt tcgtgaagcc gtccagtgaa tagcagcggg ttccgaccgg gcagcttcct
     4681 tcgtcaaagg tgcactgcgg ggtgcagaac atttcggttt ccgaaacctg gacgcagtac
     4741 gcttcgttga agaaatcttc cgtgccgtcg ttacagtccg cgttggtcgc gcaggacgcg
     4801 caaagatcca tcggtccttc cggaacgatg gcctcggcgc cctcgcagtc ttcgtcggcg
     4861 gcgcacgttt cggtgcacat gccgtcggtg cagacgtacg attcgcagtc ggcgttcatg
     4921 cggcagtacc cgtacgggaa gttgggcgtc gggaagccct tgttgcagac cttgtcaacg
     4981 catcccatgc cgtgcgggca gtcgagttcg gtcgtgcatg ccttgctgca gtagtattcg
     5041 ccaaggtatc cgtcggccag gcacatctcg tcgcattccc acgcatattc gcaggtgtcc
     5101 tggatgtcct tgccaccgac cagttcggcc ggaacgcagc gcttgtccgt gtcgctgtac
     5161 gcgaagcaga tgccgtccgg gcagtccgaa tcgatatcgc aaaccttgtt gcagacgccg
     5221 ttcaggcagt cgaatccgtc cgcgcactgc cattcccaga cgcacgcctc gccgtaaccg
     5281 gcgccgccgt caaccgtctt cggcacgcag gcaagatcct cgaactggac ggcctcggca
     5341 aggacgcgca ccgaggtggc ggcttccggg aaagcgacct cgaacttgaa ccagtcctgg
     5401 cccgcggctt ccatcgtcgc cttgaccctg aacggggccg tcttcgcttc cgcgccggcc
     5461 ttgtcgtcgt ttgcctcgac ctcatcgacg attgtctcgg ccatggcctg ttcgccaagt
     5521 cgaacgtcaa ggtagtacgg gccagtgata acgtcatcga cgcccatgac gccgatgaaa
     5581 tatgtgccgg cttcctggac gtagtatgaa attctggaaa ggttgccttc gccggcgttg
     5641 tcgtcggtcg ccacggcagc ctccgcgccg tccttccaca attccatcat ggtgtcgatc
     5701 cggatccagc ctacctggcg gcattcgccg tcggtgcagt catcgcagcc ggtgatgcac
     5761 ctggtggtgc cggccacgtc gcggcaggtt ccgaccgagc attcataatc gtgcatgcat
     5821 tcggcgccga acggcttgtt caaagacgtg tcggcgacac agatcgcaac gtcgccgttg
     5881 tatgcgttca ggacgcactc gaagccgtcc gggcaggaat ccaggtcttc tccgaaaatg
     5941 aagcactggt tgctgcagta accttcgtaa cagaagtggt tgttgcaatc cagggggctc
     6001 tggcacgggg caccctcgcg gccttccgcc tcgggcacgc agtaaaactc gaactggaat
     6061 ccgttatcgg ttccaccgtc ttcgcacgcg ccgcattcgc cgcacttggc cgagtccttg
     6121 gtgccgccgg cctcgctttc gcagttcatg gtgcagaagc gggtggtgtc aaatccaaga
     6181 cacaggccgc ccgcgcagtc ttcgtcgcgg ttgcacggct tgaacagttc gatcgttccg
     6241 tcgcccgggt agttgtcggt tccgatgcgc atgcagaagt actcgttgtc cgcgtttttc
     6301 gtgcagcgca ggccgtccgg acaggccgcc gtctcgcagc gctgggtgca gaagtgatcc
     6361 cagccccagc cgtcggccat gcacaggccg gaagcacatt ccgaatcgaa gttgcagggc
     6421 atgccggcca cctggtcgta cgccctgaag tcacactcgc cgaacggctg gcaaacgcca
     6481 aggatgcacg cgccgctgcc gtcgattcca ggctgcgcct cgcatgaggt gccgttggga
     6541 agcgcgccgg aaaggtcgaa ttcgcaggcg ccagtctcga ggttgcacgt gccgttgtcg
     6601 aacaggcact tgccgtcggg ggcgcaaacc acgtcaacgg cgacgcaggc gccggactgg
     6661 catacggggg tcttgcagtt gccggcgcct tccatcgcgc aggccccgcc ttcgttgatt
     6721 acgttgcagc cgtcgcagca gtcgtcggcg accgtgcagg tacagacaac gtcggtgcct
     6781 tcatccgggc gaacgtccgg tggggtcgta tccggtggga cgtcctgcat cgagtccatt
     6841 tgtgaatccg gcaacgtcac gtcgtagatg acgtccggct ccccgtcgcc gccgccgccg
     6901 caggcgacaa gcgttgtcgc catgaaggcg accatgatgg aaactgacag aaccctgaag
     6961 gcacgattca tattcgatac ctggtcaaag attggacgat tctacacccg gcggggcgga
     7021 ctctcaacac aggatttgac tttatgaacg gcgaaaaacc cggtcttc
//
LOCUS       DAOPPA010000112         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11910_length_7005_cov_4.035108, whole genome shotgun sequence.
ACCESSION   DAOPPA010000112 DAOPPA010000000
VERSION     DAOPPA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11910_length_7005_cov_4.035108"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..985
                     /locus_tag="PKH54_04875"
                     /pseudo
     CDS             1..985
                     /locus_tag="PKH54_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552584.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            1011..2267
                     /gene="gspF"
                     /locus_tag="PKH54_04880"
     CDS             1011..2267
                     /gene="gspF"
                     /locus_tag="PKH54_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOC99257.1"
                     /translation="MPLFEYRGISAKGKQVTGVSEADGVAELTSKLKREGIYVTRVTE
                     SGGKSPRAGKPQSGSVLSKEVDFSRFFDRVKSADVAVMTRQFATLLKAGVPMVESLAA
                     LVDQQESRKLKTMLGQIRERVREGASLADGLSDHRNVFPDLYINMVRVGEASGTLDMV
                     LERLADFLESSVRLKSKITSAMVYPIIMTCVGILIVSLMMAFVVPKMTEMYADMGGDL
                     PLATRLLKGASDLLRATWFVLIPAIFGTVYALRRYFRTEAGRLKWDRFMLRVPVFGRL
                     NRLVAVARFGRTLSTLLSSGVPVLTSLEIVRTIVNNEIMARVIDDARVAVREGDSLAS
                     PLKASGQFPPMMTHMITIGEKTGQLEAMLKNVADSYDAEVDAKVSTLTAILEPVMIVG
                     MALMVAFLIAALLMPMLQMNELISVG"
     gene            2321..2737
                     /locus_tag="PKH54_04885"
     CDS             2321..2737
                     /locus_tag="PKH54_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOC99258.1"
                     /translation="MNARSLLMHRVCRVTAARGMTLIEIMVVIAIIGIVMTAVGVGVV
                     PQLNKARIKAAAAQLNTIENALTTYSLDADTPNSLEELTKGGGALLKAKQLKDPWGTL
                     FTYAYPSSDSDREYDLCSNGPDKRAGTDDDICNYEK"
     gene            2770..3600
                     /locus_tag="PKH54_04890"
     CDS             2770..3600
                     /locus_tag="PKH54_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOC99259.1"
                     /translation="MKAPCQKGFTVLELMAVTAVVMLMAVAGTVTVRAVRRADISAAA
                     SRMSAAIRYTYDTAVLNNGTYRLVIDIASGSWWAERVDTSETCSTTAVLPGEDEEDAE
                     DGDEDRPGLRSGSNPFDGGSMATRMWDMAAIAAGSGEDGGGSGGTLAAAVAEGVASIN
                     QKGPSQPASARVEKSDEAREAARELRRQRQRVRDELLKKSDLPSGIQFKAVATGHGDE
                     PVEEGLAEIYFFPSGYVERAYIYLQLGEDVYTIETVPLRGTGVIHKEELDSREVFRKG
                     "
     gene            3597..4490
                     /locus_tag="PKH54_04895"
     CDS             3597..4490
                     /locus_tag="PKH54_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOC99260.1"
                     /translation="MRGGAVTGFRHAIDVIISTRRGRATGVRGFTLLEVMVAMTILAM
                     GIGVISYANSAAMTQISRVTRMTTASFLMESVVNDIQAYYVKEGFPSNDLEGRGCELP
                     NEFSDVFECSYDLLAMDLEPSQMQELMNVGMEAFTGSMGSLAGGEEGAAAGISGMDVG
                     RMAALAPLFGPMGGEIMSLCDINLSQLMMGITMLSSYMPQIIDQVAQRTRKLTVTLSW
                     RDGPRGQRDFTIETFIVSLPEELIRQARDQEMAREAAEEIQGSVQSSAPSAGGSGGTG
                     GGVGNLLQTLTGTAGAAGGLK"
     gene            4487..5239
                     /locus_tag="PKH54_04900"
     CDS             4487..5239
                     /locus_tag="PKH54_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOC99261.1"
                     /translation="MTPEVERTRHARAGFTLLEVMIAVGLLVVVSLLMYQSISITMRA
                     REEITRVEDLNHCAQVLLSHIKADISMAFVSNHVNIEQPASNTLFVGREDSILLTTFS
                     HERRQRGTRESDQAVVEYRVGTMDGEDVVFRREKAILDEDPEKGGVEEVIAVGVRDFK
                     LSYWDDKEEDWVDEWKVDMKDARQAGLAGGLSEAVAPVGAKFMKQAQEKMLEEYMLPS
                     RVYVRLVLADYDGNEYPFETQVPVHIRAPLNF"
     gene            5252..6499
                     /locus_tag="PKH54_04905"
     CDS             5252..6499
                     /locus_tag="PKH54_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOC99262.1"
                     /translation="MGRKANNRGVALIVVLTGITILAAFSSEFTYRSRVDIRTAMNQQ
                     RRVQAYFHARSGMEIGRLVITSQKMVDQALSSFGLSGRAAGLELWRFAPKFVDVFTSS
                     SLEFLGFKLMDLSGVRGIGVGAGGFELEIVPEDSRINVNSGSSDKDKREIFSKLYPIL
                     AGQIDEEPMTGMDRKAAEVILNIIDWADPDDSRSDIDSNGNLLSTVGGSENMNYSKWG
                     YTSRNARMDSLDELLMIEGVTDSIFCRFSRMLTVYNTSKINVNDADPWLIKAVVCDNL
                     VTDKLAVCGYNYMSPGSVMDQAMYLFETCRQIKQQLNMPPFMSASQFVNFWTRLPEPL
                     NQLVVVNPETLRASAGTKSTVLRVVSRGWVDESGHEMSAVIDTGSTRWLSWKERGFDA
                     SGKRDMGQVDGVDSAGEAGTEGR"
     gene            6502..>7005
                     /locus_tag="PKH54_04910"
     CDS             6502..>7005
                     /locus_tag="PKH54_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99263.1"
                     /translation="MGHRVIGLTRSGTILRAAVVETRLRRFELRTVLEIDLAQPGDEV
                     DEAGAVDASDGLLTVGEALNRALVPPLSPSDSVVVAYPGDAGFIRRLSFPFRDRARIS
                     EVLPVELGGTLPTGLDTELHVNFERAGKDGNDIIAVGVQAQAFASWLDGWRFQGIDPK
                     HVAMESLE"
BASE COUNT         1433 a         1867 c         2288 g         1417 t
ORIGIN      
        1 aagattatgg gcaacctgaa catcgccgaa aagcgtcttc caggacggtc gaatccgaag
       61 ggtcgtggcc ggaaaggaaa tcgacgtccg cctatcctcg gttccgacgt ctttcggtga
      121 acgcctggtt cttcgaatac ttgacaagtc ctcgacgatt ctggacttga cggatctcgg
      181 gttcgacggc gacaagctga aggtgatgca tgagctgata acaaggccgc atggaatcat
      241 ccttgtcacc ggcccgaccg ggtccggcaa gacgacgacc ctttatgcct gcctgacaag
      301 gattaatacc ccggacaaga acatcctgac ggtcgaggat ccggtcgagt atcagattaa
      361 cgggatcgga caggtgcatg tcaacaccaa gatcgggcta accttcgcaa gcggtcttcg
      421 cgcgttcctg cgccaggatc cggacgtcat cctgatcggc gaaattcgcg accgtgaaac
      481 cgcggaaatc gccgttcagg cgtctcttac aggtcacctg gtcttttcaa cgcttcacac
      541 aaatgacgca tcgtcggcgt tcacccgcct ggtcgatatg gggatcgagc cgttcctgat
      601 tgcaagttcc gtgctggcca cggtggccca gcgccttgtc agggtggtgt gtcgcgagtg
      661 ccgcgaacgg tacgaacctt cccgcggcga gcttgaaacc cttgggatcg accggatccg
      721 gccggaggac acattctaca gggctgtcgg atgtccggcc tgttcgatga cgggatacag
      781 cggacgaaac gcgatttacg agatcctgaa ggtcgaagac caggtcaggg aactggtcat
      841 gaagagcgcc gacgcctcgg aaatcaagcg tatggcgatg tcactgggca tgagaaccct
      901 gcgccaggac ggcgttgaca aggtcaggcg gggcatcacg acggccgagg aagtgatgcg
      961 ggtcacgcag ggcgacgatg aatgagccgg gttcgcccgg ggagccgtag atgccgcttt
     1021 tcgaatacag gggaatatcg gcgaagggaa agcaggtcac gggagtgtcc gaagccgacg
     1081 gtgttgccga acttacgtcg aagctgaagc gcgaaggcat atacgtcacc agagtcacgg
     1141 agtccggcgg aaaatccccc agggccggaa agccccagtc cggttcggtg ctttcgaagg
     1201 aagtcgattt ttcaaggttt ttcgatcggg tgaagtcggc cgacgtcgcc gtgatgaccc
     1261 gccagttcgc cacgttgctg aaggccgggg ttcccatggt cgagtccctg gccgcccttg
     1321 tcgatcagca ggaaagccgg aaattaaaga cgatgctggg ccagattcgc gaaagggtgc
     1381 gcgagggcgc gtccctggcc gacggactga gcgaccacag gaacgtcttt cctgatctgt
     1441 acatcaacat ggtcagggtc ggcgaggcct ccggcacgct tgacatggtt cttgagcggc
     1501 tggccgactt tctcgagtcg agcgtgaggc ttaagtccaa gataacgtcg gcgatggtct
     1561 atccgatcat aatgacgtgc gtcggcatcc tcatagtatc cctgatgatg gccttcgtcg
     1621 taccgaagat gaccgagatg tacgccgata tgggcggtga cctgccactt gccacaaggc
     1681 ttcttaaagg cgccagcgac ctgcttcgag caacctggtt tgtacttatt ccggccatat
     1741 tcggcacggt ttatgcgctt cgaaggtact tcaggaccga ggccggccgc cttaagtggg
     1801 atcgtttcat gctccgggtt ccggtcttcg ggcggctgaa ccggctggtt gcggtggcaa
     1861 ggttcggccg taccctttca accctgcttt cttccggggt tccggtcctt acttcgcttg
     1921 agatcgtcag gacgattgtg aataacgaaa tcatggcaag ggtcatcgac gacgcgcgtg
     1981 tcgcggtcag ggagggagac agcctggcga gccccctcaa ggccagcggt cagtttcccc
     2041 ctatgatgac ccacatgatc accatcgggg aaaagacggg ccagctcgag gcaatgctga
     2101 agaacgtcgc cgattcttat gacgccgaag tcgatgcgaa ggtttcgacg ctcacggcaa
     2161 tcctggagcc cgtgatgatc gtcgggatgg cactgatggt ggctttcctg attgccgcat
     2221 tgctgatgcc gatgctgcag atgaacgaac tgatttccgt cgggtagttt tccgggccgc
     2281 tgacaccgtt ccagcggtcc gttcaagcag gagaattgga atgaatgcca gatcccttct
     2341 gatgcatcgt gtgtgtcgcg tcactgccgc gcgcggcatg accctgatcg aaatcatggt
     2401 tgtcatcgcg attatcggca tcgtcatgac cgcggtcggc gtgggtgtcg ttccccagtt
     2461 gaacaaggcc aggatcaagg ccgcggcggc gcagttgaac acgatcgaaa acgcgttgac
     2521 gacatactcc ctggacgcgg acacccccaa ttccctggag gaactgacca agggcggggg
     2581 cgcgctcctg aaggccaagc agttgaagga tccgtggggc acgctgttta cgtacgcgta
     2641 tccctcgtcg gacagcgacc gtgagtacga cctgtgctcg aacggtccgg acaagcgggc
     2701 aggcacggac gacgacatct gcaattatga aaagtgatgt ccgtccgccc gccagaggat
     2761 tgccggcaca tgaaggcgcc ttgtcaaaag ggtttcaccg tcctggaatt gatggcggta
     2821 accgccgtcg tcatgctcat ggccgttgca ggcacggtga cggtgcgggc cgtccgtcgt
     2881 gccgacatca gcgcggccgc gtcgagaatg tcggcggcaa tcagatatac ctatgacacg
     2941 gccgtcctca acaacggaac gtaccgcctt gtaatcgata ttgcctccgg ctcgtggtgg
     3001 gccgaaaggg ttgataccag cgagacatgt tccaccaccg ccgtcctccc gggagaggac
     3061 gaggaggacg ccgaggacgg tgatgaggac aggccgggac tccggtctgg gtcaaatccc
     3121 tttgatggcg gttcgatggc gacacggatg tgggacatgg ccgcaatcgc ggcaggttcg
     3181 ggtgaagacg gcggcggcag cggcggaacc ctggcggcgg cggtcgcgga aggcgtcgcg
     3241 tccatcaacc agaagggccc ttcgcagccg gcttccgcgc gcgtggaaaa gtcggacgaa
     3301 gccagggagg ccgcccggga gttgaggcgg cagcggcagc gcgtccgcga cgaactcctg
     3361 aagaagagcg acctgccttc tggaatacag ttcaaggccg tggctaccgg ccacggggac
     3421 gaaccggtcg aggaagggct ggccgagata tatttctttc ccagcggcta tgtggagagg
     3481 gcctatatct acctgcagct tggggaggac gtctatacga ttgaaacggt tcccctgcgt
     3541 ggaacagggg tgattcacaa ggaagaactc gactccaggg aagtattcag gaagggatga
     3601 ggggtggggc tgtgacggga ttccggcatg ccatcgatgt gattatttcg acccggcgcg
     3661 gtcgcgcgac cggcgtccga ggcttcaccc tgctggaagt catggtcgcg atgaccatac
     3721 ttgcaatggg aatcggggtc atctcgtatg cgaattcggc ggcgatgacc cagatttcgc
     3781 gggtcacgcg gatgaccacc gcatccttcc tgatggaaag cgtcgtcaac gacatccagg
     3841 cctattacgt taaggagggg tttccctcga atgacctcga gggccgtggc tgtgagcttc
     3901 ccaatgaatt ctcggacgtg ttcgagtgct cctatgatct tctggccatg gatctcgagc
     3961 cttcccagat gcaggaactg atgaatgtcg ggatggaggc gttcaccggc agcatgggca
     4021 gtctcgccgg cggcgaggaa ggcgccgcgg ccgggatttc cggcatggat gtcggacgca
     4081 tggcggcgct ggcaccactg ttcggtccga tgggcggcga aatcatgtcg ctttgcgata
     4141 tcaacctgag ccagttgatg atgggaataa cgatgttgtc gtcctacatg ccccagatca
     4201 tcgaccaggt cgcccagagg acacggaaac tcaccgttac gctttcatgg cgggacggtc
     4261 cccgcggaca acgtgatttc acgattgaaa cttttatcgt gtcgctgcct gaggaattga
     4321 tccggcaggc gcgcgatcag gaaatggcgc gcgaggctgc cgaggagatc cagggttccg
     4381 tccagagttc cgcgccgtct gctggtggct ccggcggaac aggtggcggt gtcggcaacc
     4441 tgctgcagac gttgaccggc acggctggcg ccgcgggagg cctcaaatga ccccggaagt
     4501 cgagaggacg aggcacgccc gagccggttt cacgctcctt gaagtcatga ttgcggtcgg
     4561 tctgctggtc gtggtgtcgt tgctgatgta tcagtcgata tccataacga tgagggcgag
     4621 ggaggagatt acccgcgtgg aggatctgaa ccattgcgcc caggttctgt tgtcccacat
     4681 caaggccgac atatcgatgg ctttcgtgtc caaccacgtc aacatcgagc agccggcctc
     4741 gaacactctg ttcgtcgggc gcgaggattc gatcctcctg acgacctttt cgcacgagag
     4801 gcggcagcgc ggaaccaggg agtcggatca ggcagtggtg gagtaccggg tcggaaccat
     4861 ggatggtgaa gatgtggtct tcaggcgcga gaaggcgatt cttgacgagg atcccgagaa
     4921 gggcggcgtc gaggaagtga ttgcggttgg cgtgcgggac ttcaagcttt cctactggga
     4981 cgacaaggaa gaggactggg tcgatgagtg gaaggtcgac atgaaggatg caaggcaggc
     5041 ggggcttgcc ggcggccttt ccgaggcggt tgccccggtc ggcgcgaagt tcatgaagca
     5101 ggcccaggag aagatgcttg aggagtacat gcttccgtcc agggtgtatg tgagactcgt
     5161 cctggccgat tatgacggca acgagtatcc gtttgagacg caggttccgg tgcatatccg
     5221 ggcgccgttg aacttctagg agttaatgcc gatgggccgt aaggcaaaca acaggggcgt
     5281 ggcgctgata gtcgttctga ccggcatcac catccttgcc gcgttttcat cggagttcac
     5341 ataccgctcg agggttgaca tcaggacggc gatgaaccag cagaggcggg ttcaggctta
     5401 ttttcacgcg agatccggga tggagatagg cagactggta atcacttcgc agaagatggt
     5461 tgatcaggcc ctttccagtt tcggcctttc aggaagggct gccggtctgg agttgtggcg
     5521 gttcgcgccc aagttcgtcg atgtcttcac gtcatcgtcg cttgagtttc ttggtttcaa
     5581 gctgatggat ctttccggag tgcggggaat cggcgtcggg gccggcggct ttgaactgga
     5641 gattgtgccc gaggattccc gcatcaacgt caactcggga agttcggaca aggacaagcg
     5701 ggagattttc tccaagcttt acccgatcct ggcgggccag atcgacgagg agccgatgac
     5761 tggaatggac aggaaggccg ccgaggttat cctgaacatt atcgactggg cggatcccga
     5821 cgattccagg tccgacatcg attcgaacgg caacctgctt tcaacggtag gcggcagcga
     5881 gaacatgaac tattcgaaat ggggttacac gtcccgtaac gccaggatgg attcgctgga
     5941 tgaactgctg atgattgaag gcgtgacgga cagcatattc tgtcgtttca gccgcatgct
     6001 gacggtctat aacacttcga agatcaacgt gaacgacgcc gatccctggc tgatcaaggc
     6061 agtcgtgtgc gacaacctgg tcaccgacaa gctggcggtc tgcgggtaca actacatgtc
     6121 gcctggatcg gtcatggatc aggccatgta cctgttcgag acgtgcaggc agataaagca
     6181 gcagttgaac atgcccccgt tcatgtcggc ttcgcagttc gtcaatttct ggacgaggtt
     6241 gccggagccc ctgaaccagc ttgtggttgt caatcctgaa acgttgaggg cgtcggccgg
     6301 tacgaagagc accgttctca gggtggtgtc gcgagggtgg gtcgatgagt ccgggcacga
     6361 gatgtctgcc gtcatcgata ccggatcgac cagatggctt tcgtggaagg agcgcgggtt
     6421 cgatgcctcg ggaaaacgcg acatggggca ggtcgatggc gtcgattccg ccggagaggc
     6481 gggaacggaa ggaagataag catgggtcac agagtaatcg gtctgacaag gtcgggaacg
     6541 attcttaggg cggccgtcgt ggagacgagg cttcgcaggt tcgagttgag aaccgtgctg
     6601 gagatcgatc tggcgcagcc gggcgacgaa gttgacgaag ctggcgcggt cgatgcctcc
     6661 gacgggcttc tcaccgtggg cgaggctttg aatcgcgccc tggtgccgcc gctgagtccg
     6721 tctgattcgg ttgtcgtcgc ctatcccggc gatgccggct tcataaggag gctgtccttt
     6781 cctttcaggg acagggccag gatttcggaa gtcctgccgg tcgaattggg cggcacgctt
     6841 cccacaggtc ttgataccga gcttcatgtc aatttcgagc gggcgggcaa ggacggaaac
     6901 gacattatcg cggttggcgt ccaggcacag gcgttcgctt cgtggcttga cggctggcgc
     6961 tttcagggaa tcgatccgaa acatgttgcc atggaatcgc tcgag
//
LOCUS       DAOPPA010000113         6973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11968_length_6973_cov_4.072420, whole genome shotgun sequence.
ACCESSION   DAOPPA010000113 DAOPPA010000000
VERSION     DAOPPA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11968_length_6973_cov_4.072420"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="PKH54_04915"
     CDS             complement(<1..2017)
                     /locus_tag="PKH54_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC99264.1"
                     /translation="MYCGSDRFLADLHIHSRFSRATSRDLTIPAILRACQMKGIDLVA
                     TGDFTHPEWFGEIRNSLVEDGGGLLEAAPSIAGQVSGLVPEQARRPVKFIISGEISSI
                     YKKNGRTRKVHSLVFMPSLESASRFSIALGRLGNITSDGRPILGLDPMRILQLAMDIN
                     PGAFLIPAHIWTPHFSVFGAMSGFDTIEECFEDLTPYIFAVETGLSSDVGMNRLWSAL
                     DRFSLTSSSDAHSVSKLGREATIFSGQRSWDGLVSALKGLPGPTRLEGTVEFFPEEGK
                     YHLDGHRKCGFSCEPSESMRLGGICPVCGGRLTPGVLGRVAQLADRTEVPAGFPGQIG
                     LVPLGEVLSGILGCGEGSGRVQSAIDSLVARFGSELDILGWIDPGQLDADREYALAEA
                     VRRIRDGRVVREPGFDGEYGRIGIFGPGEVEMIKGQGQMFGTRAPTRRRREGVARAGI
                     AAVPEDLFDCPRADDVVPSSSGLTPEQAAAANCLGCNAIVVAGPGTGKTRTMVARVAA
                     LVESGVAPASILVVTFTNRAAGEAAERITAVVGIGPVVTTFHALALSMLKRAARERGQ
                     ELPQVVVDVSPDAELPVGHLTLDGLVPAFIREMRLDPSLASCFAHILVDEFQDISDDQ
                     YEMLRLMAAGGATVFCVGDPDQSIYGFRGAVPQVFDRFQADWPGCFRH"
     gene            complement(2094..3488)
                     /locus_tag="PKH54_04920"
     CDS             complement(2094..3488)
                     /locus_tag="PKH54_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99265.1"
                     /translation="MRIAIQSTVVVALAIMFSSSAFAGTWEITNQIQGGFIFMLEVPD
                     ETHAFGFGSIPDPADPQNDKFTGFKMNPNGTWGEYAPPSSGDMFVPSAIQCTAPDNCL
                     MLASDITMEPMKMETRLMLSKDGGNKWTTFQKPFWYDSRLPSGFSAFGKELVIYHSAG
                     TIAYMSHTGGPASDNQSWFVPKVGDTQYLSIQGSFILNDNVGYVMNQSMTMDSQGNPV
                     SINPGAALLKTSDLQMPEPRDMAWETLFTDQTIYARKLHFVNENVGYMITDDLVNYGL
                     SRTTDGGRNWTPIVFPQVGELAPIQWVNDIAVFDEDNLFVVAGIPNGEEDSFGVIFRI
                     SDGVTPVPVLPDPTEHQMTMQVIRCVTPYYCLAAGEEATVLRFTDEPPVVVEEDSDIA
                     IVSDGADRDAFAGDDQGGQIEDVEEGIDLKGWTPPKKDSGCSTGNGPGTGPAGLALLI
                     LGCALCLAWRRRQA"
     gene            complement(3785..4558)
                     /locus_tag="PKH54_04925"
     CDS             complement(3785..4558)
                     /locus_tag="PKH54_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC99266.1"
                     /translation="MKKLTIVLVAGAVLVGGCGIKQEDYLRDTNALKEQISTLERQKG
                     DLVTQRGRLNEDLERLRQEKSLLAQEKNVLAQKEGALSEEVTNQLKEIERLRRQAEER
                     KAKLAQLRNLLDSMVVSGKLSVRTDKGRMIVEIGEQFLFDSGEYKIKEVGRQALAQLV
                     GLLSGIEGREFQVAGHTDSVGSDEKNWRLSVNRAMEVVLYMVESGMPAGRLSVAGYGE
                     TKPTGDNETPEGRALNRRIEIVLQPNIDEILGFGEEASK"
     gene            complement(4555..6204)
                     /locus_tag="PKH54_04930"
     CDS             complement(4555..6204)
                     /locus_tag="PKH54_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOC99267.1"
                     /translation="MNAPGRLRQIASILSRYGFDDLAARLGLVPFCGAIIRRIRGGRS
                     SDLPDAAERARMVITDLGPTFVKLGQMLATRPDLLPADFIREFKKLHDEVPPFPFEQV
                     KQVFLSQTGRSLSDVFASLDEVPLAAASIGQVHAGTLLDGRKVVLKVQRPGIAGQVDV
                     DLRMLRWLAALLERRVPMFARMNARAVVEEFRRNLLRELDFQVEVANQIRYADQFVDD
                     PTLFVPRPVTEFCTSRVIVMDFVDGFKVTDTESIKAAGIDLKSVVENGMRIILRSIFE
                     FGFFHADPHPGNFFVMKDGVVALLDYGTVASVDSRRIDEMTSFMLAVAASDVDMLINV
                     LVDMDVISDDTDVRILRADLSSLMGQWRGAALRDVRMDAFMAGVIEAARRHSVRIPAD
                     LLMVGKAIAIIEGIGWEVCPDFRPVEFIQPYLQKLSVDRMLDADKQARSLARVGMEAL
                     GLLKEAPYDIRRILGRIRRGEHQIIVRNAGDRDAFRHRGILVNRAIFAFMFCAFFFGS
                     AWMIGSGDSTRNAFGVLSAVLSGMAFIGFLWSMFSFRGGRR"
     gene            complement(6260..>6973)
                     /locus_tag="PKH54_04935"
     CDS             complement(6260..>6973)
                     /locus_tag="PKH54_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC99268.1"
                     /translation="LIMADESPGYLSIVIMAFNEGATIRRQAMDSLNFLDRHVPGGSG
                     EVIIVDDGSADDTGRIADELALSDARVRVVHHGTNRGMGAAIASGYGAASCRFVTQLP
                     GDAQVHPETLARFLPLLESHDLVLSEYEKRGDGPLRAFITWGYQTTALLLLKNRCAFT
                     GTMVFRRSLIEGMPIRSRTFMANVEVPLRMLRAGVEPGMVTISCVARQAGRSKVLSVR
                     RILRVILEMMALRRELVVS"
BASE COUNT         1547 a         2183 c         1927 g         1316 t
ORIGIN      
        1 catgcctgaa acaaccgggc cagtcggcct ggaatctgtc aaatacctgt ggaacggcac
       61 cgcgaaagcc gtaaatcgac tgatccgggt cgccgacaca aaaaacggtg gcgccgccag
      121 cggccatcag gcgcaacatc tcatactggt cgtccgaaat gtcctggaat tcgtcaacaa
      181 ggatgtgcgc gaagcacgat gccagagacg ggtcaagccg catttcccga atgaatgccg
      241 ggacaaggcc gtccagtgtc aggtggccga cgggaagttc ggcgtcgggt gacacgtcca
      301 cgaccacctg gggaagctct tgcccccttt cccgcgcggc cctcttcaac atcgaaagcg
      361 ccagcgcgtg aaatgtcgtc acgaccggcc cgatgcccac cacggccgta attcgctcgg
      421 cggcctcgcc tgcggcccgg ttcgtgaaag tcacgaccag gattgaggcg ggggcaactc
      481 cggactccac cagggcggca acccttgcca ccattgtcct ggtctttccg gttcccgggc
      541 ccgcgaccac aatggcattg cagcccagac aattcgccgc ggcggcctgt tcgggagtaa
      601 gaccggatga tgacggcacc acgtcgtctg cgcgggggca gtcaaaaaga tcctccggga
      661 cggcggcgat gcccgccctg gcaacgcctt cccgacggcg cctggtcggg gcccgggttc
      721 cgaacatctg gccctgcccc ttgatcattt cgacttcacc gggcccgaaa attccaatcc
      781 ttccgtattc gccgtcaaac cccggttcgc gaaccacacg tccatcccgg attcgcctga
      841 cggcctcggc cagtgcgtac tcacggtcgg catcgagctg ccccggatca atccacccca
      901 gtatgtccag ttccgaaccg aagcgcgcca caagactgtc gatcgcggac tgaacccggc
      961 cggatccctc accgcatcca aggatgccgg aaagaacctc gccaagaggc accaggccaa
     1021 tctggccggg aaaaccggcg ggaacttcag tcctgtcggc aagttgcgcc actcttccaa
     1081 gaacgccggg ggtcaacctg ccgccacaca ccggacatat gccgccaaga cgcatggatt
     1141 ccgacggttc gcacgaaaat ccacacttgc gatgaccgtc caggtgatat ttgccttcct
     1201 cgggaaagaa ttcgaccgtt ccctccaggc gggtcggccc gggaagccct ttcagcgccg
     1261 aaacaagacc gtcccaggat cgctggccgg aaaaaatcgt ggcctcgcga ccaagtttgg
     1321 atactgaatg cgcgtccgat gacgatgtca gcgaaaacct gtccagcgcc gaccagagac
     1381 gattcattcc cacgtcggaa gacagcccgg tttcgaccgc gaagatataa ggcgtcagat
     1441 cttcaaagca ttcctcgatc gtgtcgaagc cggacatggc gccgaatacc gaaaaatgag
     1501 gcgtccagat atgggccgga atcaggaacg cgcccggatt gatgtccatg gccagttgca
     1561 ggatccgcat cggatcaagg ccaagtattg gacggccatc cgaagtgatg tttccaagtc
     1621 ggccaagcgc gatcgaaaac cttgaagccg attcgagcga cggcatgaag accagcgagt
     1681 gaaccttcct ggttcgaccg ttcttcttgt agatcgatga aatctcgcct gaaatgatga
     1741 atttcaccgg gcggcgggcc tgttccggaa ccaggccgga aacctggccg gcaatcgacg
     1801 gcgccgcttc cagcaggccg ccaccgtcct cgaccaggct gtttcgaatt tcgccgaacc
     1861 actcgggatg ggtgaaatcc ccggtcgcca ccagatcgat acccttcatc tggcacgccc
     1921 tgaggatggc cggaatggtc agatcacgac tggtcgcacg ggaaaacctg gaatgaatgt
     1981 gaagatcggc aaggaagcga tctgaaccac agtacatggc aacaacccgc gcgtcggtga
     2041 tgacaggttc gaccatggtg tccggtcggg catgcctcgc ccggacgtca ggctcaggcc
     2101 tgtcggcggc gccatgcaag gcacagggca cacccaagga tcagcagcgc aaggccggcc
     2161 ggcccggttc cgggaccgtt gccggtcgaa cagccggaat cctttttcgg tggagtccag
     2221 cccttcaggt cgatgccttc ctcgacatcc tcgatctggc cgccctggtc atcaccggcg
     2281 aatgcgtcgc gatcggcacc gtcggacacg attgcgatgt cgctgtcttc ttcgacgaca
     2341 acgggcggtt cgtcggtgaa cctcaggacg gtcgcctcct cgccggcggc aaggcagtaa
     2401 tacggagtca cacaacgaat gacctgcatg gtcatctgat gctcggtcgg atccggcagg
     2461 acgggaaccg gcgtcacgcc gtccgaaatc ctgaagataa cgccgaagct gtcttcctcg
     2521 ccgttgggaa ttccggcgac gacaaaaaga ttgtcctcat caaatacggc gatatcgttg
     2581 acccactgga tgggagccag ttcaccaacc tgcggaaata cgattggcgt ccagttccgg
     2641 ccgccgtccg tggtgcggga caggccgtaa ttcaccaaat cgtcggtaat catgtacccc
     2701 acgttttcat tcacgaaatg aagcttgcgg gcataaatcg tctgatcggt gaacaatgtc
     2761 tcccatgcca tgtcacgtgg ctctggcatc tgcagatcgg aagtcttcag aagggctgcc
     2821 ccgggattga tggaaactgg attcccctgg ctatccattg tcatgctctg attcatcacg
     2881 tatccgacgt tgtcgttcag aatgaaggag ccctgaatcg aaagatactg ggtatcgccg
     2941 accttcggaa cgaaccagct ctgattgtca ctggccggtc ctcctgtgtg ggacatgtat
     3001 gcaatcgtgc cggccgaatg ataaatgacc agttccttgc caaacgccga aaagcctgac
     3061 ggcagcctgc tgtcgtacca gaaaggcttc tggaaagtcg tccacttgtt gccgccgtcc
     3121 ttggaaagca tcaaccgcgt ttccatcttc atcggctcca tggtgatgtc gcttgccagc
     3181 atcaggcaat tatcaggggc cgtacactga atggcgctgg gaacaaacat atctccagac
     3241 gaaggtggcg cgtactcccc ccacgtcccg ttcggattca tcttgaatcc ggtgaacttg
     3301 tcgttctgtg gatcggccgg atccgggatg cttccgaatc caaaggcatg ggtttcatcc
     3361 ggaacctcca gcataaatat gaaaccaccc tgaatctggt tggtgatttc ccacgtgccg
     3421 gcaaaggccg aggacgaaaa catgattgcc agagcgacaa caaccgttga ctggatcgca
     3481 atcctcattg tggaacctcc gggatgaaac agagtacgac ggcgttattt gacacgattt
     3541 cttcaacttt tgccagacgt atcaagcgat ccgacggcgc tgaggcaacg gcatgacagg
     3601 tacgcggcgg gcggattttc attcgcccgt acggatcgcg gggcggatcg ggccggacca
     3661 taagtgtccg gtgaggcctc tcgaaccgga cacttcatgg ccggccacag gcacgactgc
     3721 gtcattcatc gagcgtggaa ttccgaatcg tggggcgtgc ggcgtggggc atacggcaga
     3781 caaattactt cgaggcttcc tcgccgaacc caaggatttc atcgatattg ggctgcagga
     3841 cgatctcgat gcgacggttc agtgcccggc cttccggagt ttcgttgtcg ccggtcggct
     3901 tcgtttcccc gtaaccggcg accgacagcc tgccggcggg catgccggat tcgaccatgt
     3961 aaagaacgac ttccatcgcg cgattcaccg acaggcgcca gttcttctca tcgcttccga
     4021 cgctgtcagt atgaccggca acctggaatt cccggccctc gatgccgctg agcagcccga
     4081 cgagttgcgc cagcgcctga cgaccgactt ccttgatctt gtactcaccg gaatcgaaca
     4141 ggaactgctc accgatctcg acaatcattc gccccttgtc ggtacgcacg ctgagtttgc
     4201 ctgaaacaac cattgaatcc agaaggttcc gcaattgggc cagtttcgcc ttgcgttcct
     4261 cggcctggcg ccgaagccgt tcgatttcct tcaactgatt ggtgacttct tccgacagcg
     4321 cgccttcctt ctgggcaagc acgttctttt cctgggccag aagcgatttt tcctggcgca
     4381 ggcgctccag atcctcgttc agacggccac gctgggtgac cagatcgccc ttctgcctct
     4441 cgagcgtcga aatctgttcc ttcaaggcat tggtgtcacg aagatagtct tcctgcttga
     4501 tgccacatcc accgacaagc accgcgccgg cgacaagaac gatagtcagc ttcttcatct
     4561 gcgacctccc ctgaaagaaa acatggacca aaggaaaccg atgaacgcca tgcccgaaag
     4621 gacggcggac aatacaccaa acgcattacg cgtagaatcc ccggaaccga tcatccacgc
     4681 ggagccgaaa aagaacgcgc agaacatgaa tgcgaagatg gcccggttga caaggatacc
     4741 ccgatgtcgg aaggcatccc gatcgcccgc gttcctgacg atgatctggt gttcgccgcg
     4801 cctgatcctg cccaggattc gtcgtatatc gtagggggct tccttcagca ggccgagcgc
     4861 ttccatgccg acccgggcaa gcgaccttgc ctgcttgtcc gcgtccagca tgcgatcgac
     4921 ggaaagcttc tgcaggtatg gctgaatgaa ctcgaccggt ctgaagtccg gacagacctc
     4981 ccagccgatg ccctcgatga tggcaatcgc cttgccgacc atcaggagat ccgccggaat
     5041 acgaaccgaa tggcgccttg ccgcctcaat cacgccggcc atgaaagcgt ccatccgcac
     5101 gtcgcgcagg gcggccccgc gccactgccc catcagcgag gaaagatcgg cgcgcagaat
     5161 cctgacatcg gtgtcgtcgc ttatcacgtc catatcgaca agaacgttaa tcagcatatc
     5221 gacatcggac gcggccacgg ccagcatgaa cgacgtcatt tcatcgattc gccgcgagtc
     5281 aacagacgcg accgttccat aatcgagcaa ggcgacaacg ccgtccttca tcacgaagaa
     5341 gttgcccgga tgcgggtcgg cgtgaaaaaa cccaaattcg aatatggagc gaaggataat
     5401 gcgcatcccg ttttcgacaa ccgacttaag gtcgattccg gcggccttga tcgattcggt
     5461 gtcggttact ttgaaaccat cgacgaaatc catgacgatg acacgcgacg tacagaattc
     5521 cgtcacgggc cgcggcacga acagcgtcgg gtcatcgacg aactggtcgg catacctgat
     5581 ctggttcgcg acctcgactt gaaagtcgag ttcccgaaga agattccggc gaaactcctc
     5641 gaccaccgcc cgcgcgttca tccgggcgaa catcgggaca cgtctttcaa gaagggctgc
     5701 cagccagcga agcattcgaa gatcgacatc cacctgtccg gcgatgcctg gtcgctgaac
     5761 cttcaacacc accttgcggc catccagcag cgtgcccgca tgaacctgac cgatcgacgc
     5821 ggccgccagc ggcacttcat ccaggctggc gaagacgtcg ctcaaagacc gcccggtctg
     5881 agacaggaag acctgcttga cctgctcgaa cggaaacggc ggcacttcgt cgtgaagctt
     5941 cttgaattcc ctgatgaaat cggcgggaag aagatccggc ctggtggcca gcatctgacc
     6001 gagcttgacg aaggtcggac ccagatcggt gatcaccatc ctggcccgtt cggccgcatc
     6061 cggcaaatcg gaagagcgcc cgccgcgaat ccttcgaatg atggcgccac agaacggcac
     6121 aaggccaaga cgcgcggcaa gatcgtcaaa gccgtatctg gacagtattg acgcaatctg
     6181 gcgcaggcgt cccggagcgt tcataaaatc aatcccgggg aaacctcaag acgcgcgatt
     6241 tcccctctga cgggcccggt caggatacca caagctcccg tcgaagggcc atcatctcaa
     6301 ggatcacgcg caggattcgt cgaaccgaaa gcaccttgct ccgaccggcc tgcctggcga
     6361 cgcaactgat cgtcaccatg ccaggttcaa cgcctgcccg aagcatccga agcggaacct
     6421 cgacatttgc catgaacgtg cggcttcgaa tcggcattcc ttcaatcagg cttcgccgaa
     6481 acaccatcgt tccggtaaag gcacacctgt tcttcagaag aagaagggcc gtcgtctggt
     6541 atccccaggt tatgaacgcc ctcaacgggc cgtcgccccg cttttcatat tccgaaagca
     6601 ccagatcatg cgattccagc agcggcagga acctggcaag agtttccgga tgaacctgtg
     6661 cgtcaccggg aagctgagtc acgaaacgac atgatgcggc cccgtaccca cttgcaattg
     6721 cggcgcccat gccgcggttc gtgccgtgat gaacgacgcg aaccctggca tccgacaagg
     6781 ccagttcatc ggcgattctg ccggtgtcgt cggccgaccc gtcatcgacg atgatcacct
     6841 cgccactccc ccccggaaca tgccgatcaa ggaagttcaa cgagtccata gcctggcgcc
     6901 tgatcgtcgc gccctcgtta aacgccatga taacgatgga aagatacccc ggactttcgt
     6961 cagccattat caa
//
LOCUS       DAOPPA010000114         6965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11981_length_6965_cov_3.665847, whole genome shotgun sequence.
ACCESSION   DAOPPA010000114 DAOPPA010000000
VERSION     DAOPPA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11981_length_6965_cov_3.665847"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PKH54_04940"
     CDS             complement(<1..1960)
                     /locus_tag="PKH54_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99269.1"
                     /translation="MRNIAWVLAVGLVAGVVGCGDGLAGTDTLDEGGIDVPADQGAGD
                     VATDVADDAGRDASTDVEADATDVPGDATQYDVLDVTDTGDAALDVSEDATPPYWTDE
                     PFENEFAGFDRGDYQPILGWILLDYNREKILADIETAAGWGVNHIQLSHDIIMDVDEL
                     LGDDQATLDRVETINQAIAAAHNHGMKVYLWAHEFQDGDIVVCYGPDGAIWDARAAAY
                     RDAIARVPDVDGIVLMFGSAGASPWFTLCDCDWCADTFPDEMMPPPNDVKIQLVIEHI
                     GDVLEGLDKEMVARVFAHEPDENAWHADGLARARDTGFVSMHKSEVQDWQPYNPIDPT
                     LGRVGAHPSILEMDAAGEYFGRSELPFASPLYYRYRLKAAYDKTAIGAVARISRGSDT
                     ALGTPNEVNLLTIRRYVQNPETSIAQVWEEFIKARYLPPEIWTRDEQNALIRILEMTL
                     PIMTKTHYVLGLWALEKGSDIPSEPVTSELGGRGDMPKWNPVWQGIWDLVKQPDLNTV
                     WQIWVEATEAVQLAQQALDQFRNREWAMSQDDQDDLYRRLNHQYYAARAFRAVKMYIF
                     ARKSAVFYPINSALMYTWSDWAREELADVAEEMGNWGLDDVSLVNPDRIIRFLGTTAD
                     APDTTPSYPGDPMTRPLEFLSVDATSVTV"
     gene            2244..4289
                     /gene="fusA"
                     /locus_tag="PKH54_04945"
     CDS             2244..4289
                     /gene="fusA"
                     /locus_tag="PKH54_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOC99270.1"
                     /translation="MAAKDKPKGMERIRNIGIIAHIDAGKTTVSERLLYVTGKIHKIG
                     EVHDGAAVMDYMIQEQERGITITSAVTTFEWKGRDVHVIDTPGHVDFTIEVERSLRVL
                     DGAVVVFDGVSGVEPQSETVWHQADKYRVPRIAFINKLDRIGADFEKAVDSMRGRFTQ
                     KIVPIQIPIGAESDFAGVIDLMEMRAMTWDGDDPSEFETMEELPAALADMAGVMREEM
                     ISAIADFDETIGDRFLMGEDISVEELKDALRRVTISGDVVPVLCGSALRNKGIPPLLD
                     AISDYLPSPKDVPQVVGVNPRTGESVTMPHDPRGPLCGLVFKVQVADDGRRLAYVRIY
                     SGSLDEKSDVFNPARNKKEKLSRIFMMHARERIRVEEIGAGNVVGVLGLKDTGTGDTI
                     CDADNQIVLERIGGYEPVISMAVEADAQSDREKLQETLARIGDEDPTFRFREDEGTGQ
                     MVMSGMGELHLDIVADRIRRDFGIPVRVGRPQVVYAETITTDASGFGECDIFNDDVKI
                     YCKLSLVVKPAARGAGVTCTVQPGLLAQPNYQNAARVGVVEALQGGVLHGYTMTDIDA
                     VVTGIEQREGCQFNETVVKIAAMNAVREVCGQAGPARMAPIGLINVVCPADTMGDVIA
                     TLQARRGSVTNMEERGATKIIEAEAPVERMFGYSTELRSVTQGRATFSMVFGRYDIE"
     gene            4427..5305
                     /locus_tag="PKH54_04950"
     CDS             4427..5305
                     /locus_tag="PKH54_04950"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOC99271.1"
                     /translation="MSRAIPILVAAMLLPVLSGCQINKLVADNMSASMDDQKESFYRE
                     KSVRHAREAAPAMLKLLDGFIVSSPDNYDLLIRGAEMNCGFSMLMIEADDQEWASVLY
                     MKGYDYAIRALKHRIPDIDSILAAGGEERLKEALAKLDTKAVPEIFWAGLCLGSHMNI
                     NLDDVSAMANLSRVLTFIDRALNLSPDFFYGGPEMFLGVYYGTLGEMLGGKPALSKKH
                     FENAFQISNGRFLVAKIFFAKTYCVQIQDRELFERTLNEVLEAPDDMAPELALVTAAA
                     KKQAEALLERVDELFI"
     gene            5337..6371
                     /gene="dctP"
                     /locus_tag="PKH54_04955"
     CDS             5337..6371
                     /gene="dctP"
                     /locus_tag="PKH54_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOC99272.1"
                     /translation="MKKLNWNGLAGRITGCLAVATFLAVSLAVSFEASAEPVVIKLAT
                     SAPTGSLWFKELNKIGSVTEKLTEGRVKIKIFGGSVLGDDKQVLDKMKYGQVQAAAMT
                     GVGLGDLVPKTRLMELPFIFDNPKQIDCVLSKVLPDFNKELEKAGFINLGWAEQGLIY
                     VFTSRDIKTLADMQKTKPWVWGSDPLARAIWEVFNVKPVPLALQDVYMSLQSGIIDTA
                     YISPAAVTGLGWHQKAPNMLDVPVTNGAGALLLKKDIWDKISPADQEVLKKTADQVMS
                     RLVAKSREENAKAINVLQEKGGVKLVLVPASERPGFLSKGAESADRLVGKLYTKQELE
                     AFRAAVKACR"
     gene            6396..>6965
                     /locus_tag="PKH54_04960"
     CDS             6396..>6965
                     /locus_tag="PKH54_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOC99273.1"
                     /translation="MNPIVKAIRAFDTALSRVETVAIVVVVAAMMLLTTGHVVLKLAG
                     LGVIQLEEAARYLVIWVGFLGGSVAAYQSRHINIDILTRFIKGVPKRVSLCLVWLIGV
                     GITVVLAKTAISYVGDLKEAGGIAIAYGDPGSELQVPEWILAVIMPAGLILIAWHMLA
                     GAVYAALGMPGPGHDAAAAAAGKTDTGAAD"
BASE COUNT         1401 a         1960 c         2180 g         1424 t
ORIGIN      
        1 gcaccgtgac cgatgttgcg tcaaccgaaa ggaattccag tggccttgtc atcgggtcgc
       61 cggggtagga cggagtcgtg tccggtgcat cggcggttgt tcccaggaac cggataatcc
      121 tgtcagggtt aaccagcgac acatcgtcga gtccccagtt gcccatctct tccgcgacat
      181 cagcgagctc ttcccgggcc cagtcggacc atgtgtacat cagcgcggaa ttgattggat
      241 aaaacaccgc gctcttacgc gcaaagatat acatcttgac cgcccggaag gcccttgctg
      301 cgtaatactg atgattcagc cggcggtaaa ggtcgtcctg atcatcctgg gacatggccc
      361 attcacgatt cctgaactgg tccagcgcct gctgggccaa ctggacggcc tcggtagcct
      421 cgacccagat ctgccagacc gtgttcagat ccggttgctt gaccaggtcc cagatgccct
      481 gccacaccgg gttccattta ggcatgtccc cgcgaccgcc caactcagat gtaacgggct
      541 cggacgggat gtccgaacct ttttccagcg cccacaggcc caggacgtag tgcgtcttcg
      601 tcatgatcgg gagtgtcatt tccagtatcc gaatcagtgc gttctgttcg tcgcgagtcc
      661 agatctccgg cggcaggtag cgggccttaa tgaactcttc ccagacctgg gcgatggatg
      721 tttccgggtt ttgaacatac cttcggattg tcagcaggtt aacttcgttc ggcgtgccaa
      781 gggcggtgtc ggatccacgt gatatgcggg cgacggcgcc gatcgccgtc ttgtcgtatg
      841 cggccttcag ccggtacctg tagtaaagag gggatgcgaa gggcagttcg gaccgtccga
      901 agtattcgcc ggccgcgtcc atttccagga ttgaggggtg tgccccgacc cggccgagcg
      961 taggatcgat cggattgtac ggctgccagt cctggacctc ggacttgtgc atgctgacaa
     1021 atccggtgtc gcgtgcccgc gccagcccgt ccgcgtgcca agcgttttca tccggctcgt
     1081 gcgcgaagac ccgggcaacc atttccttgt ccagtccctc cagcacgtcg ccgatatgtt
     1141 caatcacaag ctgaatcttc acgtcgtttg gcggcggcat catttcatcc gggaatgtgt
     1201 ccgcgcacca gtcgcagtcg cacagggtga accacgggct tgccccggcc gacccgaaca
     1261 tcagcacgat tccgtcaacg tcgggaaccc tggcaatcgc gtcgcggtag gcagcggccc
     1321 ttgcatccca gatagcgccg tcaggcccat agcagacaac gatgtcgcca tcctggaact
     1381 cgtgcgccca caggtaaacc ttcatgccgt ggttgtgggc ggccgcgatg gcctggttaa
     1441 tcgtttccac gcggtcgagc gtcgcctggt cgtcgccaag cagttcgtca acgtccatta
     1501 tgatgtcgtg cgaaagctgg atgtggttga ccccccatcc cgctgcggtt tctatgtctg
     1561 ccaggatctt ttcccggttg tagtccagaa gtatccatcc gagaatcggt tggtaatccc
     1621 cgcggtcaaa ccccgcgaac tcattttcaa acggttcgtc cgtccagtag ggcggggtag
     1681 cgtcttccga cacatccagc gcggcatcgc cggtatccgt aacgtcgagg acatcatatt
     1741 gggtcgcgtc ccccgggaca tcggtcgcgt cggcttccac gtccgtggac gcatcccgcc
     1801 cggcatcgtc ggcaacatcc gtcgcaacgt caccggcccc ctgatccgcg ggcacatcga
     1861 tcccgccttc atccagggtg tccgtcccgg ccaacccgtc cccgcacccg accacgcccg
     1921 caaccagccc gaccgccagc acccacgcga tattcctcat gccctcacca acccctcaga
     1981 cccccggatg ctaacccgat tgccataacg tgtcaccgtt caatgccctt ttggctcgct
     2041 tccttcgact cgcgttgctt cccaggcggg cgaattttca ttcgcccgta cggattgggg
     2101 gggcggacgg ctgctccccc tcccctgagc gttgcgaagg gcgccctggc agatacaaat
     2161 cgtctttaca agacgggttt cgaagcgggg cgggttgtgg cataattccc gcgccccatt
     2221 tgcgaatgac gacgggaacc gacatggcag cgaaagacaa accgaagggc atggagagga
     2281 tccggaatat cgggatcatc gcccatatcg acgcgggcaa gacaacggtc agcgaacgac
     2341 tgctgtatgt aaccggaaaa atacacaaaa tcggtgaagt ccacgatggc gcggccgtca
     2401 tggactacat gatccaggaa caggaacgcg gcatcacgat caccagcgcg gtcacaacgt
     2461 tcgagtggaa ggggcgcgac gtgcacgtga tcgacacacc cggccacgtc gatttcacga
     2521 ttgaagtcga acgcagtctg cgtgttcttg acggcgcggt cgtcgttttt gacggcgtca
     2581 gcggcgtcga gccgcagtcg gaaaccgtgt ggcaccaggc ggacaagtac cgcgtgccca
     2641 gaattgcctt tatcaacaag ctcgatcgaa tcggcgcgga cttcgaaaag gcagtggatt
     2701 cgatgcgcgg gcgattcacg cagaagattg tgccgatcca gatcccgatt ggcgccgaga
     2761 gtgattttgc cggggtaatc gacctgatgg aaatgcgggc gatgacctgg gacggtgacg
     2821 acccgagcga gttcgagacg atggaggagt tgcccgcggc gctggccgat atggccgggg
     2881 tcatgcgcga ggagatgatt tccgccatcg ccgattttga tgagacgatc ggtgacaggt
     2941 tcctgatggg tgaggatatc tctgtcgagg aactgaagga cgcgttgcgc agggtgacca
     3001 tctccggtga cgttgtgccg gtgctttgtg gcagcgccct gcggaacaag ggaattcccc
     3061 cgctgctgga cgcgatttcc gattacttgc cgtcgccgaa ggatgtgccc caggtggtgg
     3121 gcgtcaatcc aaggactggt gaatcggtca cgatgccgca cgacccccgg gggccgctgt
     3181 gcggcctggt tttcaaggtg caggtggccg acgacggtcg gcgacttgct tacgtgcgca
     3241 tatattcggg aagccttgac gaaaagtcgg acgtcttcaa tccggccaga aacaaaaagg
     3301 aaaagctttc ccgcattttc atgatgcatg cccgcgaaag aatcagggtc gaggagatcg
     3361 gcgccgggaa tgttgtcggg gttcttgggt tgaaggatac cggtaccggc gacacgatct
     3421 gcgatgccga caaccagatc gttcttgaaa ggatcggcgg ctatgagccg gtcatcagca
     3481 tggcggtcga ggcggacgcc cagtcggacc gcgagaaact gcaagagacc ctggcgagaa
     3541 tcggcgacga ggatccgacg ttccgtttcc gtgaggacga ggggaccggg cagatggtca
     3601 tgtccggaat gggcgagctt cacctggaca tcgtcgcgga tcgtatcagg cgggatttcg
     3661 gcattcctgt gcgcgtgggc cgtccgcagg tcgtttacgc ggaaaccatc acgactgacg
     3721 cttccggatt cggtgaatgc gacatcttca acgacgatgt gaagatttat tgcaagctga
     3781 gccttgtggt caagcccgcg gcccgtgggg ccggcgtgac atgcaccgtt cagcccggcc
     3841 tgctggccca gcccaactac cagaacgcgg cccgggtcgg tgttgtcgag gcactacagg
     3901 gtggcgttct tcatggttac acgatgaccg atatcgatgc cgtggttacc ggcatcgaac
     3961 agcgcgaggg atgtcagttc aatgaaaccg tagtgaagat cgccgcgatg aacgcggtcc
     4021 gggaagtctg cgggcaggcc ggcccggcca gaatggcgcc gattggactg attaatgtcg
     4081 tctgtccggc ggatacgatg ggtgacgtca tcgcgaccct gcaggctcgg cggggcagtg
     4141 tcacgaacat ggaagagcgc ggcgcgacca agattatcga ggccgaggcg ccggtggaga
     4201 ggatgttcgg atactcgacg gaactgcggt cggttacaca gggcagggca acgttcagca
     4261 tggtgttcgg gcgctatgat attgagtgac ggcttgcgcg atcgcgtttg acgcggccgc
     4321 gtcatggcaa taacatggga accgcccggg cgatcggttt tgcgattgtt ccgtgggggc
     4381 gaatgaaaat tcgcccgttc ctttgacggc gcggacagga gggcacatgt ccagagctat
     4441 tccgatactt gttgcggcaa tgcttctgcc ggtgctgtcc ggctgtcaga tcaacaagct
     4501 ggttgccgac aacatgagcg cctcgatgga cgaccagaag gaatccttct atcgggaaaa
     4561 gagcgttcgg catgcccgcg aggcggcgcc ggccatgttg aagctgcttg acggattcat
     4621 cgtcagttcc ccggacaatt atgatttgct gatccgcggc gccgaaatga attgcgggtt
     4681 cagcatgctg atgatcgaag cggacgatca ggaatgggcg tccgtccttt acatgaaggg
     4741 atacgactac gcgatacgcg cgctgaagca ccgcattccc gatatcgatt caatactggc
     4801 ggcaggcggc gaggaaaggc ttaaggaagc gcttgcgaag ctggacacga aggccgttcc
     4861 cgagatcttc tgggccggac tctgccttgg aagccacatg aacatcaacc ttgatgatgt
     4921 ctcggcaatg gcaaaccttt caagggttct tacgttcatc gatcgtgccc tgaatctttc
     4981 cccggatttc ttctatggcg gacccgagat gttccttggc gtgtattacg gaacacttgg
     5041 cgagatgctc gggggcaagc cggctttgtc aaagaagcac ttcgagaacg cgttccagat
     5101 ttcaaacgga cgcttcctgg ttgccaaaat attcttcgcg aagacttact gtgtgcagat
     5161 tcaggacagg gaattgttcg agcggacgct gaacgaagtg ctcgaggcac cggacgacat
     5221 ggccccggag cttgcgcttg tgaccgcggc cgccaagaag caggccgagg cgctcctgga
     5281 aagggttgat gaattgttca tctgatattc gagggttttc cttaagggga taaaatatga
     5341 aaaagctcaa ttggaacggt cttgctggca ggattaccgg ctgcctggca gtcgcgacct
     5401 ttcttgctgt gtcacttgcg gtatcgtttg aggcgagcgc cgagcctgtg gtcattaaac
     5461 tggccacgtc ggcccccacc ggatcgctgt ggttcaagga actgaacaag atcggaagcg
     5521 ttaccgagaa acttaccgaa ggccgtgtaa agataaagat tttcggcggc agcgttcttg
     5581 gtgacgacaa gcaggtcctt gacaagatga agtacggcca ggttcaggcg gcggcgatga
     5641 ccggtgtcgg gctgggcgac ctggttccaa agaccaggct gatggaactg ccgttcattt
     5701 tcgacaaccc gaagcagatt gactgcgtgc tgtcgaaggt gctgcctgat ttcaacaagg
     5761 aactggaaaa ggccggtttt atcaatctgg gttgggccga acagggcctt atctacgttt
     5821 tcacgtccag ggatatcaag acgcttgccg acatgcagaa gacgaagccg tgggtctggg
     5881 gttcagaccc gctggccagg gctatctggg aagtgttcaa cgtgaagccg gttccgttgg
     5941 cgctgcagga cgtatacatg tcgcttcaga gcggcatcat cgacaccgcg tacatcagtc
     6001 cggcggccgt caccggtctg ggctggcacc agaaggcgcc gaacatgctc gatgtgcctg
     6061 ttactaacgg cgcgggcgcg ctgctgctga aaaaggatat ctgggacaag atcagtccgg
     6121 ccgatcagga agtcctgaag aagaccgccg atcaggtcat gtccagactg gttgcgaagt
     6181 cccgcgagga gaacgcgaag gccattaacg tcctgcagga aaagggcggc gtgaagctgg
     6241 ttctggttcc cgcctcggag cggcccggct tcctgtcgaa gggtgccgaa tccgccgaca
     6301 ggctggtcgg caagctgtac acgaagcagg aacttgaggc tttccgcgcc gcggtcaagg
     6361 cctgccgctg acatccaacc agagggaatt aatccgtgaa tccaatcgtc aaggctatcc
     6421 gtgctttcga tacggcactg tccagggtcg aaacggttgc catcgtggtg gttgtggcgg
     6481 ccatgatgct gctgaccacc gggcacgtcg tgctgaagct ggccgggttg ggcgtcatcc
     6541 agcttgaaga ggcggccagg tacctggtca tctgggtggg gttcctgggc ggctcggtcg
     6601 ccgcgtacca gtctcgacac atcaacatcg atatccttac caggttcata aagggcgtgc
     6661 caaagcgtgt cagcctgtgc ctggtgtggc tgatcggcgt cggcatcacg gttgtcctgg
     6721 caaagacggc gatttcgtac gtcggggatc tgaaggaggc cgggggcatt gcaattgctt
     6781 acggcgaccc tggcagcgaa cttcaggttc ctgaatggat tctggccgtg atcatgccgg
     6841 cgggactgat actgattgcc tggcacatgc tggcgggtgc ggtttatgcc gcgcttggca
     6901 tgccggggcc gggtcatgac gccgcagcgg ccgcggcagg caagacggac acgggggccg
     6961 ccgat
//
LOCUS       DAOPPA010000115         6962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_11989_length_6962_cov_4.200232, whole genome shotgun sequence.
ACCESSION   DAOPPA010000115 DAOPPA010000000
VERSION     DAOPPA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11989_length_6962_cov_4.200232"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PKH54_04965"
     CDS             complement(<1..161)
                     /locus_tag="PKH54_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOC99274.1"
                     /translation="MDTASPLKLTTQIGKTDFWNDSCSISELEYAISHGAVGATTNPT
                     IVLGVLKKE"
     gene            complement(164..1645)
                     /gene="mmsA"
                     /locus_tag="PKH54_04970"
     CDS             complement(164..1645)
                     /gene="mmsA"
                     /locus_tag="PKH54_04970"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOC99275.1"
                     /translation="MEKKVLFNYIDGRFVAAEAASVEDVCNPSTGEVISRVPYSVPAE
                     IDCAVAAARAAFPSWSRTPVSRRVKPLFDLAHEMHRREEELCRVLVREMGKSLPDARA
                     EMKRAVENVEAACAMPLLQQGEKLVDCSADMDGEVLRMPVGVFGIIAPFNFPAMVPFW
                     FIPYAVATGNTIVVKASELVPNTMQMLFEMMHAAGIPKGVVNLVNGDRVAAEALIDHP
                     DVAGISFVGSTRVAALVAEKCAKTHKRFQALGSAKNHLVVMPDAKLDDVVRNMVTSCY
                     GCAGQRCMASSAIVTVGREIHEQVVSRFVAASREVLAADPLDPKHENEGMLMGPVISA
                     KNKAFIESMIQRGVEQGATLALDGRGLQVPGCACGHFVGPTVFTDVKPGMDIHTTEIF
                     GPVVVILNVDTLDEALKIIADHQYGNGASIYTQNGYWARKFELEARAGMIGINVGIPA
                     PVAFLPFGGTKHSAYADIKAQGRDVVRFFTEAKVITRRFWKED"
     gene            complement(1647..2690)
                     /locus_tag="PKH54_04975"
     CDS             complement(1647..2690)
                     /locus_tag="PKH54_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC99276.1"
                     /translation="MAATIKQIAGRLNVSVATVSRALNDLPGVGEKTRRKVRRTAAEL
                     GYVPDFHARALVSGKVPFLGLVVPDITNSFFPALALAVEEAAAASGQSLILLNTNWRR
                     DRLAQALELLSSRRVSGLIVAEPLDAAILDSVDLETVSNGLVLAGVEAPRGTGLCSVM
                     VDDRDGGMQVGRHLVECGAHRIAFVGGPSESPNSRDRLEGLTIGLGISGGAAAIVSVT
                     DGAWTEESGYRQADRLLRDMVPDAIFAANDLLAIGVMRRLVESGIAVGRGVALAGYDD
                     THVVSLTSVPITSVAQPTAQVGSLAVKLLGQMLSGDSGCGDAVLKPSLVARSSTLEFS
                     NLHVLAPRDAQGA"
     gene            complement(2913..4085)
                     /locus_tag="PKH54_04980"
     CDS             complement(2913..4085)
                     /locus_tag="PKH54_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC99277.1"
                     /translation="MREYERTHPWISFDLDLGRASPKFWMMLGEIQSKCEHIAGVPLM
                     GDVAKRLHQVFLAKGVLATTAIEGNTLTEEEVARRIQGLLDLPPSKDYLGREIDNVVD
                     GCNEIRRRIIGGEAGILTTAQIMDFNRRIMSGLPDSVEKLPGEIRTVSVGVGRYRGAP
                     AEDCEYLVDRLCEWMNQERQQAPADMKIAMGVVRAILAHLYLVWIHPFNDGNGRTARM
                     MELQILLDVAVPTPAAHLLSNHYNQTRSEYYRMLDRSSSDNGNAMHFVMYALQGMLDG
                     LRSQIDLIRDFQWDVAWREHVYAAFKTIPGTGESVRVRRRDLILALSNVRDPVAVPDL
                     RKISPEVAEAYAGRSIRMLSRDVEALVEMGLVEVSPSGARAARESILRFLPGRAER"
     gene            4243..4596
                     /locus_tag="PKH54_04985"
     CDS             4243..4596
                     /locus_tag="PKH54_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99278.1"
                     /translation="MAAPTFDSGFPKLPAGAAGIMFVHDFRITCRRGAGRWPLVRTMA
                     RNRRRRIFTGRRRFSGRSAVTPKPSRAGGAQAEVVVAVAGVVVVAIGSANVPGVVVPR
                     AAAINPVGALVAIDR"
     gene            complement(4641..5657)
                     /locus_tag="PKH54_04990"
     CDS             complement(4641..5657)
                     /locus_tag="PKH54_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HOC99279.1"
                     /translation="MKRRGHLFNQVTDFNHLCRSATLAAHGTFNRDSLSFMFELERNV
                     VELKRQLEGRQYLPGEYRTFCITDPKPRIISASPFRDRVVHHSLCGALEPVIESYLID
                     DCYACRVGKGTVAAIQRARLFVRRFERFAKLDVRHYFETADHQVLKAVIRRLVKDPGV
                     LWLTDQFIDKGAPGSIPGKGMPIGNLTSQNFANLYLGPFDHFMKEAVRIPGYVRYMDD
                     ILLFDDDAVRLKQAVVLATTFLEGMLLETKDEAFRTGRTSDGVPFLGFNIFPAVIRFD
                     RIRRRRFARKLAAAAAEIQSGTEIPANVMASLQSIYGWARIGDTASFARTVTAGVLPW
                     ESFG"
     gene            complement(5671..6030)
                     /locus_tag="PKH54_04995"
     CDS             complement(5671..6030)
                     /locus_tag="PKH54_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOC99280.1"
                     /translation="MIGELPAMEKWQKVGGDIMDRTAKFPKSVRFTFSTRIDGLVLEV
                     IDGLVIARYSTQRERPERLQQVSLCLSRLLALLRMAFDRGHLNAGSYEYLCSGIVEVG
                     SMVGGWRRSITQARARD"
     gene            complement(6027..6533)
                     /locus_tag="PKH54_05000"
     CDS             complement(6027..6533)
                     /locus_tag="PKH54_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOC99281.1"
                     /translation="MKAKVFTIRWDPEEQVMDDRSLQEFFTDRELVSISVNFCQIDGV
                     PTMFAATTYREARTVPGFVVGRSAGEQSGRRDPREELSAGECVVYDGLREWRNAKAVA
                     LGVAGYMILTNRQLAAVSRAMPATLAALGEIEGIGEVRLKEHGQEILDIVRRLGAADL
                     SGGGGTGT"
BASE COUNT         1450 a         2208 c         1940 g         1364 t
ORIGIN      
        1 atttccttct tcagcacgcc aagaacaata gtcggattgg tggtcgcgcc gaccgccccg
       61 tgcgaaatcg cgtactcaag ctcggaaatc gaacacgagt cgttccagaa gtcggtcttt
      121 ccaatctgcg ttgtcagctt cagggggctt gcggtatcca tattcaatcc tctttccaga
      181 aacggcgcgt gatgactttc gcttcggtga agaagcggac gacgtcgcga ccctgggcct
      241 tgatatcggc gtacgccgaa tgcttggttc cgccgaacgg caggaacgcg accggtgccg
      301 ggatgccgac gttgatgccg atcatgccgg cacgggcctc cagttcgaac ttcctggccc
      361 agtacccgtt ctgggtgtaa atcgaggccc cgttcccgta ctggtggtcg gcgatgatct
      421 tcagtgcctc gtcaagcgta tcgacgttca ggatcacgac gaccggcccg aaaatttcgg
      481 tggtgtggat gtccatgccc ggctttacat cggtgaacac ggtcggtccg acgaagtgcc
      541 cgcacgcgca tccgggaacc tgcaggccgc gtccgtccag ggcaagggtc gcgccctgtt
      601 cgacgccgcg ctggatcatc gattcgataa aggccttgtt cttcgcggaa atgaccggcc
      661 ccatcagcat tccttcgttt tcatgcttcg gatccagcgg atctgcggcc aggacttccc
      721 tggacgcggc cacgaaacgc gaaacgacct gttcatgaat ctcgcggccg accgtcacga
      781 tggccgaaga cgccatgcag cgctggcccg cgcatccgta acacgacgtg accatgttgc
      841 gcaccacgtc gtccagcttg gcatccggca tcacgaccag gtggttcttg gcagacccga
      901 gagcctggaa ccgcttgtgg gtttttgcgc acttttcggc aaccagggcg gccacgcggg
      961 tcgatccgac gaacgaaatg ccggccacgt ccgggtggtc gatcaacgct tccgcggcga
     1021 cccggtcgcc attgaccagg ttcacgacgc ctttcgggat gccggcagcg tgcatcatct
     1081 caaaaagcat ctgcatcgtg ttcggaacca gttccgacgc cttgacgacg atggtgttcc
     1141 cggtcgcgac cgcgtacggg atgaaccaga acggcaccat tgccgggaag ttgaacgggg
     1201 cgatgatccc gaaaacgccg accggcatgc gcagcacctc gccgtccatg tcggccgaac
     1261 aatcaaccag cttttccccc tgctgaagca gcggcatcgc gcatgcggcc tcgacgttct
     1321 cgacggcgcg cttcatttcg gcgcgggcgt ccggcaggga tttgcccatc tcgcgcacca
     1381 ggacgcggca gagttcctcc tcgcgccggt gcatctcgtg ggccaggtcg aacaacggtt
     1441 tcacgcgccg cgaaaccggg gtcctggacc acgatgggaa cgcggcacgg gcggcggcca
     1501 cggcgcaatc gatttcggcc ggcaccgaat acgggacgcg ggaaatgact tcgccggtgc
     1561 tcggattgca gacatcttcg acactggcgg cctcggctgc gacgaaacgt ccgtcgatgt
     1621 aattgaaaag gacctttttt tccatgtcag gctccctggg cgtcgcgtgg cgccagaacg
     1681 tgaaggttcg aaaattcaag cgtgcttgac cttgcgacca gtgaaggctt aaggaccgca
     1741 tcgccgcatc cggagtcgcc ggacagcatc tgcccaagca atttgaccgc cagcgaaccc
     1801 acctgggcgg tcggctgggc aaccgacgta atcggcaccg acgtcaacga aacgacgtgg
     1861 gtgtcgtcgt aaccggccag ggcgacgccg cggccgaccg cgatcccgct ttcgaccagg
     1921 cggcgcatca ccccgattgc cagcaggtcg ttcgccgcga agatggcgtc cgggaccatg
     1981 tcccgaagaa gacggtcggc ctgccggtat ccggattcct cggtccaggc gccatcggtc
     2041 acacttacaa tcgcggccgc gcccccggaa attcccaggc cgatggtcaa accttccagg
     2101 cggtccctgg aattcgggct ttcggacggg ccgccgacaa aggcaatccg atgggcgccg
     2161 cactcgacca ggtggcgacc gacctgcatc ccgccgtcac ggtcatcgac catcaccgaa
     2221 caaaggccgg tcccacgggg cgcttcgacg cccgcaagca ccagcccgtt cgaaacggtt
     2281 tcaagatcga ccgaatcaag aatcgccgca tcaagcggct cggccacgat caggccggag
     2341 acgcgccggg aagaaagaag ttccagggcc tgggcaagcc gatcgcgccg ccagttggta
     2401 tttaacagga tcaggctttg tccgctggcc gcggcggcct cctcgacagc aagtgcaagc
     2461 gccgggaaga acgaattcgt gatgtcggga acgaccagac ccaggaacgg caccttgccg
     2521 gaaaccaggg cgcgggcgtg aaaatccggg acatagccaa gttccgcggc cgtgcggcgc
     2581 accttgcgac gggtcttttc gccaacgccc ggcaggtcgt tcaaggcccg cgaaacggtc
     2641 gcgaccgaca cgttcaaccg cccggcaatc tgctttatcg tcgccgccat cccgccaccg
     2701 ccgaaccgcc caaaacccaa cgtaaacgat tacgttgcgt aaacgtttac gccatcccgc
     2761 cgcccttgtc aacctgccgc actgtcctcg gccttcggga tcggactttc cttggttctg
     2821 gattgggtca aggcacaaca catcgtaagg gcgaatgaaa attcgcccgc ctcatttcgg
     2881 attcacattt gcggttcttt acggacctgg cgtcaacgct ccgcccttcc gggcagaaag
     2941 cgcaaaatgc tttcccgggc ggccctggcg cctgacggag atacttcgac cagtcccatt
     3001 tcgacaaggg cttcaacatc ccgggacagc atgcggatcg atcggcctgc ataggcctcg
     3061 gcgacttccg gggaaatctt gcgcagatct ggcacggcaa caggatcccg aacattcgac
     3121 agcgccagga ttaaatcgcg tcgcctgacg cgcacggact cccccgtccc cgggatcgtc
     3181 ttgaaagcgg catagacgtg ttcgcgccag gcaacgtccc actgaaaatc cctgatcaaa
     3241 tctatctgcg aacgaaggcc gtccagcatc ccctgcaagg cgtacatgac gaagtgcatg
     3301 gcgtttccat tatcgctgct ggatcgatcg agcatcctgt aatactcgct gcgcgtctga
     3361 ttgtaatgat tgctcaaaag atgcgccgca ggcgtcggaa ccgcgacgtc cagcagaatc
     3421 tgcagttcca tcatccttgc cgtgcgcccg ttgccgtcat tgaaaggatg gatccacacc
     3481 aggtacagat gcgcaaggat tgcccgaacg acccccatcg caattttcat gtcggccggg
     3541 gcctgctgcc tttcctggtt catccattcg cacaggcgat ccaccagata ctcgcaatcc
     3601 tccgccggcg ccccacgata ccggccaacg ccaacgctca ccgtccgtat ttcccccggc
     3661 agcttttcca ccgagtccgg caggcccgac attattcggc ggttgaaatc cataatctgg
     3721 gccgtcgtaa ggattccagc ctcacccccg ataatgcgcc gccgaatctc gttgcatcca
     3781 tccaccacat tgtcgatttc ccgaccaagg tagtcttttg agggcggcag atccagcagt
     3841 ccctgaatgc gacgtgcaac ctcttcctcg gtcagggtat tgccctcgat ggctgtggtg
     3901 gccagcacgc cttttgccag gaagacctgg tgaagccgct tcgccacatc gcccatcagg
     3961 ggaacgccgg cgatatgttc acacttggac tggatttccc ccagcatcat ccagaacttc
     4021 ggactggccc ggcccaagtc aagatcaaag gaaatccagg gatgtgtccg ttcatactca
     4081 cgcattttgt caaaccgccc gtctaatata acaaaccgat aaccaaggat aattggacat
     4141 caaagcaggc tggtatgtca agcatcttgt cacaagctct gtcacagtct ttgtcacagt
     4201 ctttgtcact gcttttggat cagaaaagtc acagaatggt gcatggccgc cccgacgttc
     4261 gacagcggtt ttccgaagct accagccggg gcggccggga taatgttcgt ccacgacttc
     4321 aggataacct gccggcgtgg cgcgggtcga tggccactgg tcaggacaat ggcacggaac
     4381 cggcggcgcc ggattttcac cggcaggcgc cgattttctg gccggtcggc agtaacgccg
     4441 aagccgtccc gagctggcgg ggcgcaggcc gaggttgttg ttgcggttgc cggggtcgtt
     4501 gttgttgcga ttggcagcgc gaacgttcct ggcgttgttg ttccacgagc cgccgcgatt
     4561 aacccggttg gagcccttgt cgccatcgac cgctgaacaa atccaaacat gtcgcggccc
     4621 cgctgcacag cgactttcaa tcaaccaaag gattcccaag gcaaaacgcc tgcggtgacc
     4681 gttcgcgcaa atgacgcggt atcaccgatt cgggcccagc cgtatatgct ttgcagactt
     4741 gccatcacat tcgcggggat ttccgttcct gattggattt cggcggcagc cgccgcaagt
     4801 ttcctggcaa accgacgtcg tcggatgcgg tcaaacctga tcaccgccgg aaagatgttg
     4861 aatcccagaa atggcacccc atccgaagtc cggccggttc tgaacgcttc gtccttggtt
     4921 tcaagcagca tgccttccag aaatgttgtc gcaagcacaa ccgcttgctt caggcgaacc
     4981 gcatcgtcgt caaacagaag gatgtcgtcc atgtaacgca cataaccggg gatgcgaacc
     5041 gcttccttca tgaaatgatc aaacggacca agatacaggt tggcaaagtt ctggctggtc
     5101 aggtttccga tcggcatccc ctttccggga atggatcccg gcgcgccctt gtcgatgaac
     5161 tgatcggtca gccacagaac accgggatcc ttcacaagac gcctgatgac cgctttcaag
     5221 acctgatgat cggccgtctc gaagtaatgc cggacatcca gtttggcaaa tcgttcgaag
     5281 cgcctgacga ataaccgtgc ccgctggatt gcggccaccg tgcccttgcc gacacggcag
     5341 gcgtagcaat cgtcgatcag atagctttcg atgaccggct cgagcgcgcc gcaaagtgaa
     5401 tggtgtacga cccgatcgcg aaacggcgag gcgcttatga tccgcggctt gggatcggtg
     5461 atgcaaaacg tcctgtattc accaggaagg tattgacgcc cttccagttg ccgcttcaat
     5521 tccacgacgt tgcgttcaag ttcaaacatg aacgaaaggc tgtcgcgatt gaaggttccg
     5581 tgcgcggcca gcgtggccga cctgcataaa tgattgaaat cggttacctg attgaacaga
     5641 tggccgcgtc ttttcatcgt caatcacatt tcaatcccga gccctggcct gcgttatcga
     5701 tcttcgccac ccaccgacca tgcttccaac ttccacgatc cccgagcaca ggtattcata
     5761 acttccggca ttaaggtggc cccggtcgaa agccatgcgc aacagggcaa gcagccttga
     5821 aagacacagc gacacctgtt gaagcctttc gggcctttcc cgttgtgtcg aataccttgc
     5881 gattacaagt ccatcaatca cctccaggac aagcccgtcg attcgggtgg aaaaggtgaa
     5941 ccggactgat tttggaaatt tcgcggtcct gtccatgata tcgccgccaa ccttctgcca
     6001 cttctccatc gccggaagtt cgccgatcat gttccagtcc ccccgccacc ggacaggtcg
     6061 gcggcgccca gtcgccgaac gatgtccagt atctcctgtc catgctcctt caacctgact
     6121 tcaccgatac cttcgatttc acccagggcg gcaagggtgg ccggcattgc ccgggaaact
     6181 gcggcaagct gccgattcgt caggatcatg tatccggcaa cccccaaggc aacagccttt
     6241 gcattgcgcc attcgcgaag accgtcgtaa acgacacatt cgcccgcgga caactcttcc
     6301 ctggggtcgc gcctgccact ctgttcgccg gcagatcgac ccacgacaaa acccggaaca
     6361 gtccgggctt cccgatacgt tgtggcggca aacattgtcg gaaccccatc gatctgacag
     6421 aaattgaccg agattgaaac cagttccctg tcggtgaaga attcttgcag acttcgatcg
     6481 tccatcacct gctcttccgg atcccagcga attgtgaaca cctttgcttt cataaaattc
     6541 tcctgcacaa cctgccggaa attcttgcct gccggatacc gccaagtctt caaaaagccg
     6601 aaataacgtt aaacattcga actgttggct tccagcaaac cgacagcgaa cggcaccgtg
     6661 cccgttcgcg gcgggcggat gtaatccgcc cgtacaaggc agggcactcg acccgtaagg
     6721 gcgaatgaaa attcgcccgc ccgcaaaaaa tcgggcggcg cccggtctgg tgcggcgatg
     6781 ctttcggggc catggccctt gcgggccgcc ccgacgcctt cgccgcccca tcccgggcgc
     6841 aaggccacag gaacattcga accgcctgac ggcgcactgc taaaggtaaa agggttaaag
     6901 ggttaaaggt tatcggacag acctggcggg gcgcaggccg aggttgctgc cgcggttgcc
     6961 gg
//
LOCUS       DAOPPA010000116         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12063_length_6927_cov_4.113650, whole genome shotgun sequence.
ACCESSION   DAOPPA010000116 DAOPPA010000000
VERSION     DAOPPA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_6927_cov_4.113650"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2474
                     /locus_tag="PKH54_05005"
     CDS             <1..2474
                     /locus_tag="PKH54_05005"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOC99282.1"
                     /translation="NEIASTRGGNLARLILGVLKEPMFLMLVAAGALYLLMGEPSDAL
                     MLLGFVVVVMTITVVQERRTEHALDALRDLSSPRALVIRDGVRHRIAGREVVVGDLMV
                     VSEGDRVPADGILRRCINLCVDESVLTGESVPVRKSASETGGVMERPGGDDHSSVYSG
                     TLVTSGQGVVEVLSTGSATEFGRIGRSLASVTQESTPLQKETGRLVKLFAVVGLSACA
                     AVVVVYGLTRGGSADCWKDGLLAGIAMAMATLPEELPVVLTVFLALGAWRISRSNVLT
                     RRMPAVETLGAATVLCVDKTGTLTMNRMTLRSLSTVGCDFHLDESTSVLPEDVHMLVE
                     NAILASRRDAFDPMEKALHEVGERFLGDTEHLHPDWALVREYPLSPELPAVSQAWKLD
                     DGRLVVSSKGAPEAIADLCHMGERECEDMRRRVGELASKGLRVLAVARAVSAMRDLPD
                     RHHDLELQCIGLLGFEDPLRQGVPEAVAECGRAGIKVVMITGDYPATALAIARRAGLS
                     QSGSVITGPELEAMTDRELADRIVDTRVFARVVPEQKLRIVQALKQNGEIVAMTGDGV
                     NDAPALKAAHIGIAMGGRGTDVAREAADLVLTHDDFGSIVDAVRLGRRVFDNIRKAIT
                     FIVAVHIPIIGLSMIPVFFSSWPLLLLPIHIVFLELIIDPSCSLVFEAEDAESDVMAR
                     PPRGSSQRLFSFKSIRVALLQGFSVLAACLGVYWMATGSHSEDAARALTFGTLVVAFL
                     ALIMVNRSWSRTMWSMLRVGNRALVWVLAGTVILLPVIMFVPFARDLFHFAPPHVPDL
                     LMSLGTGMLSVVWFDLLKIRRPSLT"
     gene            complement(2471..3946)
                     /locus_tag="PKH54_05010"
     CDS             complement(2471..3946)
                     /locus_tag="PKH54_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99283.1"
                     /translation="MSTGHTRVLLTGILPCLAILSLAGCGSSSVTNDAHTDSGSDAPD
                     ATDAALDEGPADAGRDAGTTGDNSTGDDGQADLSDMEPLGPDVTPWDTTDDHGRDEGL
                     PDIPADGDAGIDDGQPDTPDTFFDSPPDPGDSEDIAECPELDCNIDCPKGFKTDGGGC
                     PTCRCRECGDMQDCFGLADFMCDNPICTPGGDCTCDCGEFADHNYVCPDGTVICMGRC
                     SDSGVTFMHDVEQECPTLCQAGRTIDLQCPDGLFQPWCECRTDDCRPECRNIGTPEEG
                     WYQPCSGEALMLTKCQGHQVFCDKIGSKSEGWYDTAGNRLSWTVCSPAWACRALSDAC
                     SSLECSGSEPSGQINCPDGRSSQFCSCTRSGAMECPANPQATCGNHWRGCARAGEFLR
                     TDAWLQCCPGTLRLRPIRDIDGTCVPDDTNPAGSCSECGDGLCESPEDRCNCPEDCLD
                     GLFPGYPGSPCLRDTECPPDLVCGNPSGHSGFCVAGDTRRR"
     gene            complement(3993..5807)
                     /locus_tag="PKH54_05015"
     CDS             complement(3993..5807)
                     /locus_tag="PKH54_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOC99284.1"
                     /translation="MTNINELRAKAHWPPGWNAARFLILAQPWLPVLRADADIESIRK
                     TLLDRINLRHFETYSDDHDIHDLDRVIVRDCARAWRGILHPRSERLAGLSVLKCMIDV
                     ATGHPRPDLEDGFWAEICHLLLGLEGFVRLHQSDSLLSRNGLSGRQAAIMRSAELDRL
                     SVVMNKASGRFSCGLEPEMMNQRENRVKGILKELDATEDDWLDWRWHLKNLATGAEKL
                     SRIATITDVERKCLDQAARAGIPVAVTPYYASLFCDRPGDADRAIRAQVIPPESYVDS
                     FNNEAGGCGPYDFMRENDTSPVDLITRRYASIAILKPINTCPQICVYCQRNWEIESPD
                     ALERISPRDRMENAIAWLDNHPGIHEVLVTGGDPLMMDNRSLGRILEGIARIPHVERI
                     RIGTRTPVTLPMRIDDDTARMLGSFKSPGFREICLMTHVEHPSEVTPDFVEAVSRLRD
                     AGVPVYNQLVYTFFVSRRFEAASLRRLLRRCGVDPYYTFYPKGKEETAEYRVPIARLL
                     QEQKEESRLLPGLARTDEAVYNVPGLGKNYLNSWQHRDLISIRPDGARVYEFHPWEKK
                     ISSQETWVGDDVPILGYLSRLASIGEDPEEYDSIWYYF"
     gene            complement(5934..6140)
                     /locus_tag="PKH54_05020"
     CDS             complement(5934..6140)
                     /locus_tag="PKH54_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99285.1"
                     /translation="MKTGCRKNGDIGLSDSFECRSRRMVVSLDKCLSDFVDADVYQRR
                     RRACFRCPEGRRNREAFAGKFIGE"
     gene            complement(6241..>6927)
                     /locus_tag="PKH54_05025"
     CDS             complement(6241..>6927)
                     /locus_tag="PKH54_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC99286.1"
                     /translation="AELNSPAKSASAPIRVMIIDDHEIFRTGLEQIIRGQPDMIFGGG
                     AGSSADAMAIIRTSPPDVVILDLSLKGRSGIDVLKDARALSGSARFLVLTVHDENLFA
                     ERALRAGASGFIMKEEGAGRVLEGIREVAAGRFFFSSSVSQKILGTMSGRAREDGRLP
                     MDRLTDRELVVFELFGRGLNTRQIAADLNLSQKTVEAHRENIKRKLGIADGQELLRRA
                     VLWVQDLNVS"
BASE COUNT         1272 a         1989 c         2168 g         1498 t
ORIGIN      
        1 tcaatgagat cgcctcgacc aggggcggca accttgcgcg tttgatcctc ggggttctca
       61 aggagccgat gttcctgatg ctggtcgcgg ccggggccct ttacctgctg atgggcgagc
      121 cttcggacgc gctgatgctt cttggcttcg tggtcgtcgt gatgacgata actgtcgtcc
      181 aggagcgccg caccgagcac gcgctggacg ccctgaggga tctttccagt ccccgggcgc
      241 ttgtcatcag ggatggggtg cgccacagga ttgccggtcg agaggttgtc gtcggtgatc
      301 ttatggtcgt atctgaaggg gatcgggtac ctgccgacgg catccttcgc agatgcatca
      361 acctttgtgt tgacgagtcc gtgctgaccg gcgagtccgt tcctgtcagg aagagtgcgt
      421 ccgaaactgg cggtgtcatg gagcggcctg gtggcgacga tcattcgtcg gtctattccg
      481 gaacgctggt aacttcgggc cagggtgtcg tcgaagtgct gtcaaccggt tccgcaacgg
      541 aattcggcag gatcggcagg tcgcttgcca gtgtgaccca ggaatcgacg ccgcttcaga
      601 aggaaaccgg ccgtctggtg aagctgtttg cggtcgtcgg gctgtctgcc tgcgcggccg
      661 tggtggtcgt gtatggcctg acacgaggcg gttccgccga ctgctggaag gacgggctgc
      721 ttgccggcat cgccatggcc atggccacgc tgcccgagga gttgccggtg gtgcttacgg
      781 tttttctggc cctgggcgcg tggaggatat ccagaagcaa cgtccttacc cggcgcatgc
      841 cggccgtgga aaccctgggc gcggcgacgg tgctatgtgt tgacaagact ggaacgctga
      901 cgatgaaccg gatgacattg cggtcattga gcactgttgg ttgtgacttc cacctggatg
      961 aatcgacgtc ggtgctgcct gaagatgttc acatgctcgt tgaaaacgcg attcttgcaa
     1021 gtcggcggga tgcctttgat ccgatggaaa aggccctgca tgaggttggt gagaggttcc
     1081 ttggcgatac cgagcacctg catcccgact gggcgctcgt cagggagtat ccgctttccc
     1141 cggaactgcc ggccgtcagc caggcctgga aactggacga cggaagactt gtcgtttcct
     1201 cgaagggggc tcccgaggcc atcgccgacc tgtgtcacat gggtgaacgc gaatgtgaag
     1261 acatgagacg gcgggtgggt gaactggctt caaagggcct gcgggtgctt gccgttgcgc
     1321 gggcagtttc cgcgatgcgg gatctgccgg atcgtcatca cgacctggaa cttcagtgca
     1381 tcgggctgct tggattcgag gatccgttgc ggcagggggt gcccgaggct gtcgccgagt
     1441 gcgggcgtgc gggaatcaag gtcgtaatga taacgggtga ttatcctgcc acggccctgg
     1501 cgattgcgcg cagggccgga ttgagtcaat ccggaagcgt cataaccggg cccgaacttg
     1561 aagccatgac ggatcgggaa cttgccgaca ggattgttga cacccgggtg tttgcgcggg
     1621 ttgtccccga gcagaagctg aggatcgtac aggcgctgaa gcagaacggc gagatcgtcg
     1681 cgatgaccgg tgacggcgtg aacgatgctc ccgcactgaa ggccgcacat atcggcatcg
     1741 ccatgggtgg acgcggaacc gatgtcgctc gcgaggcggc ggatctggtt ctgacacacg
     1801 atgacttcgg ttcgatcgtt gatgccgtaa ggctgggacg ccgggtgttc gacaacatcc
     1861 ggaaggcaat cacgttcatt gtcgccgttc acatcccgat tatcggcctg tccatgattc
     1921 cggtattctt cagttcctgg ccgcttctgt tgcttccaat acatattgtg ttcctggaat
     1981 tgataattga tccgtcctgt tcgctggttt tcgaggccga ggatgccgag tcggatgtca
     2041 tggcccggcc cccccgcggc agttcccagc ggctgttttc gttcaagtcc attcgagttg
     2101 cgctgcttca gggattcagc gtgcttgcgg cgtgtctggg ggtctattgg atggcaaccg
     2161 gcagtcattc tgaagatgcc gcccgggctc tcacattcgg aacgctcgtc gttgcttttc
     2221 ttgccctgat catggtgaac cgctcgtggt ccagaaccat gtggtcaatg ctaagggtcg
     2281 gtaaccgggc gcttgtgtgg gtgctggctg gaaccgtcat tctgctgccg gtgataatgt
     2341 ttgttccatt tgcgcgggat ctgttccatt tcgcgccgcc ccacgtcccg gatctgctga
     2401 tgagcctggg aaccggcatg ctttccgtcg tgtggttcga ccttctgaaa atcaggaggc
     2461 ccagcctgac ctagcgcctt cgggtatctc cggccacgca gaagccggaa tggccggaag
     2521 gatttccgca tacgaggtcg ggcgggcatt cggtgtcgcg caggcacggg gaaccgggat
     2581 agccgggaaa aaggccatca agacagtctt cagggcagtt gcagcggtct tcgggggatt
     2641 cgcacaggcc atcgccgcat tcgctgcagg agcccgcggg atttgtgtca tcggggacgc
     2701 acgttccatc gatatcgcgg atcggtcgca ggcgcagcgt tccggggcag cactgcagcc
     2761 acgcatcagt ccgaagaaat tccccggccc tggcgcatcc gcgccagtga tttccacatg
     2821 ttgcctgcgg gttggcgggg cattccatgg cacctgatct tgtgcatgaa cagaactggg
     2881 atgaccttcc gtcgggacag ttgatctggc cagacggttc agagcccgag cattccagcg
     2941 aagaacatgc gtctgacaac gcacggcatg cccaggcagg agaacagacg gtccaggaaa
     3001 gcctgtttcc tgccgtgtcg taccagcctt ccgattttga accgattttg tcgcaaaaca
     3061 cctgatgccc ctggcacttc gtcagcatca atgcctcgcc gctgcacggc tggtaccagc
     3121 cctcttcggg cgtgccgata ttgcggcatt ccgggcggca gtcgtccgtc ctgcattcgc
     3181 accagggttg aaacaggccg tcggggcact gaaggtcgat cgtccgcccg gcctggcaaa
     3241 gcgtggggca ttcctgctcg acgtcgtgca tgaacgtgac tccggaatca gagcaccttc
     3301 ccatgcatat gacggttccg tcgggacaga cgtagttgtg atcggcgaat tccccgcaat
     3361 cgcaggtgca gtccccgccc ggcgtgcaga ttggattgtc gcacatgaag tcggccaggc
     3421 cgaagcagtc ctgcatgtcg ccgcattccc gacaccggca agtcggacat ccgccaccgt
     3481 ccgtcttgaa gcctttcgga cagtcgatgt tgcagtccag ctcggggcac tccgcgatat
     3541 cttccgaatc cccggggtct gggggcgaat cgaaaaatgt gtcaggggtg tcgggctgac
     3601 cgtcgtcaat tccggcgtcg ccgtcggcgg gaatgtctgg caggccttca tcccggccgt
     3661 ggtcgtcggt ggtgtcccag ggagtgacat cgggacctaa aggttccatg tcgcttaagt
     3721 cggcctggcc gtcatcgccg gtcgagttgt cccccgttgt gccggcgtca cggccggcat
     3781 cggcagggcc ttcgtccagg gcggcgtcag ttgcgtcggg ggcgtcggaa cccgaatcgg
     3841 tgtgggcgtc gttcgtgacc gacgacgagc cgcagcctgc caacgaaaga atggcaaggc
     3901 agggcaggat gcctgtcagc aaaactcggg tgtgtccggt gctcatgcgc atgctccgga
     3961 ttataaacga tcccggggca aatcgataat cgtcagaaat agtaccagat gctgtcgtat
     4021 tcttccggat cctcgccgat cgacgcgagc cttgacaggt atccaaggat cgggacgtcg
     4081 tcgcccaccc aggtttcctg cgacgatatc ttcttttccc acggatggaa ctcgtaaacc
     4141 cgggcgccgt ccggcctgat tgagataagg tcacggtgtt gccaggagtt caggtagttc
     4201 ttgccaagtc cgggcacgtt gtagacggcc tcgtcggttc tggccagacc ggggagaagt
     4261 cgggattcct ccttctgctc ctgcagcagc ctggctatcg gcacgcggta ctcggccgtc
     4321 tcttccttgc ccttcggata gaacgtgtaa tacggatcga ccccgcaacg gcgcagaagc
     4381 ctgcgcaggc tggcggcctc gaaacgcctt gagacgaaga aggtgtaaac aagctggttg
     4441 taaaccggca cgcccgcgtc gcgcagcctt gaaaccgcct cgacgaagtc gggtgtcacc
     4501 tcggatggat gctccacgtg cgtcatcagg cagatctcgc ggaatccagg actcttaaag
     4561 gagccgagca tccttgccgt gtcgtcgtcg atgcgcatcg gcagcgtcac tggagtccgg
     4621 gtcccgatcc tgattctttc gacatgcggg atcctggcga tgccttcgag aatacgtcca
     4681 agtgatcgat tatccatcat cagcgggtcg ccgccggtca cgagcacttc atggatcccc
     4741 gggtggttat ccagccatgc gatcgcgttt tccatccgat cccgcgggga gatgcgttcc
     4801 agggcgtcgg gtgattcgat ttcccagtta cgctggcaat agacgcatat ctggggacac
     4861 gtgttaatcg gcttcaaaat cgcaatcgac gcgtaacgcc gggtgatcag atccaccggc
     4921 gacgtatcgt tctcgcgcat gaaatcgtac ggaccgcatc caccggcttc attgttgaag
     4981 ctgtcgacat agctttccgg tggaataacc tgggcccgga tcgcgcgatc ggcgtcaccg
     5041 ggccggtcac agaacaggga cgcataatat ggagtcaccg cgaccgggat tccggccctg
     5101 gcagcttggt caaggcactt gcgttcaaca tcggtaatgg tggcaattct tgaaagtttc
     5161 tcggccccgg ttgcaaggtt cttaaggtgc cagcgccagt caagccagtc gtcctcggtc
     5221 gcgtccagtt ccttgaggat tcctttcacg cggttctcgc gctgattcat catctcaggc
     5281 tccaggccgc aggaaaagcg tccggaagcc ttgttcatga cgaccgagag cctgtccagt
     5341 tcggctgagc gcatgattgc cgcctgccgt cccgaaaggc cgttgcggga aagaaggctg
     5401 tccgactggt gcagcctgac aaatccctcg agtccgagaa gcaggtggca gatttctgcc
     5461 cagaatccat cttcaaggtc gggacgcggg tgacctgtcg cgacatcgat catgcacttg
     5521 agcacgctga gaccggcaag ccgctcgctt cgcggatgaa ggattccccg ccaggcgcgg
     5581 gcgcaatcgc gaacgatgac acgatcgagg tcgtgaatgt cgtggtcgtc ggaatatgtc
     5641 tcgaaatgac gaaggttgat tcgatcaaga agggttttgc ggatggattc gatgtccgca
     5701 tccgcccgca gaaccggcag ccatggttgg gccagaatca ggaagcgcgc ggcgttccat
     5761 cccggcggcc agtgggcctt tgcccgcagt tcatttatgt ttgtcatcgg gttctcccta
     5821 tgtgttgcca ggtgaatcgg cacctgtaac agattctttc cacatggcgg gtcactgtca
     5881 aaattatctt gttaattcgt tcaattacag tggcttcggt cgggacgccc ggatcattcg
     5941 ccgatgaatt ttcctgcgaa ggcctccctg ttccggcgac cttcgggaca tctgaagcac
     6001 gctcggcgtc ggcgctgata gacatccgcg tcaacgaagt cggaaaggca cttgtccagc
     6061 gaaacgacca tcctgcggct gcggcactcg aaagagtcgg acagcccgat atcaccgttc
     6121 ttgcggcaac cggtcttcat ggcttccccc ttcagatttt ttggcagacc attgaagtat
     6181 cgcagcatcc ggatttcact tggattggag gattacctga atgtcgcata gggaaaatcc
     6241 ctatgaaacg ttaagatcct ggacccacag aaccgcgcgc cgcaggagtt cctggccatc
     6301 ggcaatgccc agcttgcgct tgatgttctc gcgatgcgcc tcgacggttt tctggctgag
     6361 gttgagatcc gcggcaatct ggcgggtgtt caggccgcga ccgaaaagtt cgaataccac
     6421 gagttcccgg tcggtcagtc gatccatggg cagccggccg tcttcccgtg cccgcccgga
     6481 catcgtgccg aggatcttct gggacacgga gctggaaaag aagaaccggc cggcggcaac
     6541 ctccctgatg ccctcgagaa cccggccggc gccctcctcc ttcatgatga agccacttgc
     6601 gccggcccgc agggcgcgct cggcgaaaag gttttcgtcg tggaccgtca gaaccaggaa
     6661 cctggcactg cctgaaagcg cccgagcgtc cttcagcacg tcgattcccg atcttccctt
     6721 cagggaaaga tccagtatca ccacgtcagg tggcgatgtc ctgatgattg ccatcgcgtc
     6781 ggcactggat ccggcgccac ccccgaagat catgtccggt tgtccgcgga tgatttgctc
     6841 aagtccggtt ctgaagattt cgtgatcgtc aattatcatg acgcggatgg gggcagaggc
     6901 ggattttgcc ggagagttca gttctgc
//
LOCUS       DAOPPA010000117         6918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12084_length_6918_cov_3.668367, whole genome shotgun sequence.
ACCESSION   DAOPPA010000117 DAOPPA010000000
VERSION     DAOPPA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12084_length_6918_cov_3.668367"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PKH54_05030"
     CDS             complement(<1..1383)
                     /locus_tag="PKH54_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOC99287.1"
                     /translation="MKGIRISRVLLSLAVVVAVISVAGACRRPARLVLSKDQQARIQE
                     SILDAAPTPEHVVNANFGDKVELIGVDMKPANPAPGQEVTVTWYWHCTRKVQDGWKVF
                     VHLELPKGKRMGLDHVPVGELFPMDRWEPGQYVKYQMNFKIDAKQEVGRAVMWAGLFS
                     VDLYNSRGTGERMIVVNRDQVKHDGENRVNVVEFQMNRSATPPAPDTRLTVNPVIGSA
                     PVIDGALDEEAWKGAARIVRLGDAFGKPISGEEGTSVMVMNDNDFIYFGISATDLSIE
                     ATLKNRDDQLWTEDVFEIYLDALLDGKNYLEIQVSPANVVFDALFESHRSPKWEDAAK
                     HTVEGLVSAVKLDGTINKVDDIDRGWTMEVKIPVKSIPGLASISPGTTLRANFFRMNM
                     HQGRLGRAHAWGPGGRDFHDLEKMGVLEFSTVVAVAGDKPLTEPAKAAPAAPTSEAPA
                     PAAPAAPTAPA"
     gene            complement(1427..2596)
                     /locus_tag="PKH54_05035"
     CDS             complement(1427..2596)
                     /locus_tag="PKH54_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOC99288.1"
                     /translation="MSGYSPDFKDVPKTIAARFKPVGKGPFHDVPDAQWYDWKWQLQH
                     RITDIAELGRYINLSPEEIAGHEAGRDVFKIGITPYYVALMDRDDPLCPVRMQATPHP
                     QEMISHPSDMEDPLAEDRDMPVKGLTHRYPDRVLFYMTQDCAMFCRHCTRKRKVANPH
                     TAADDAQIQGCIDYIRRTPCVRDVIVSGGDALAVSDDRLEKILAQIRDIPHVEIIRIA
                     TRTPCTMPMRITDELVAMLKKYHPIFLNTHFNHPKEATAEAFAALRKIAGAGIPIGNQ
                     MVLLKGINDDAETVMKLNHAMLMMQVKPYYIYQCDPTFGNYHFRTPVSKGIEIIKSLR
                     GFTSGLAVPYYVIDAPGGGGKVPILPEYVKSWENGVIVVRNFRGDEYTYIEPNQD"
     gene            2817..3104
                     /locus_tag="PKH54_05040"
     CDS             2817..3104
                     /locus_tag="PKH54_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99289.1"
                     /translation="MAVKEYQFNHPYDAQTAMDKIRPLLGKWSGAYKLDMEQTSPTQY
                     TLSTTGVQAEINIADNKATAVVEMNMMMEMMFRGKIEEALEQKLRPAIEKM"
     gene            complement(3107..4174)
                     /gene="nadA"
                     /locus_tag="PKH54_05045"
     CDS             complement(3107..4174)
                     /gene="nadA"
                     /locus_tag="PKH54_05045"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOC99290.1"
                     /translation="MNSDYSAVDIQAETERLHGFLKHVGWSVPECAGIAPKTLRIREL
                     ARQKRAVILGHSYVAPEVIFGVADHRGDSLGLSVIARDTDADVIVFCGVRFMGETAKI
                     IAPSRTVLLPAPNAGCSLSESITAEDVRRFRHANPDHSIVCYVNTSAAVKAECDACCT
                     SANALAVVEAAPSSRIFFVPDGRMAANLAPMTAKTIKGYTGNCIVHDCIRPEHVAALR
                     EKAGPGACVMVHTESPPEIVAMADVAGGTGDMMKAVKSGRWNTFVVITEDGMTDRFRI
                     EFPEYRFFALGTVCPHMKMIGLDQVLQVLESPRPDQVVEIDEQVRLGALKAIERMFEL
                     TGKGKDDSKPHSNTGDVAASN"
     gene            4426..5994
                     /locus_tag="PKH54_05050"
     CDS             4426..5994
                     /locus_tag="PKH54_05050"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC99291.1"
                     /translation="MKRRTRIFAIVLVVLHLAGFGIFFALRPQSGPAIPPDRKVDWVL
                     PPGQFQHKCGRVCFDTEPGNRGGFKDPDGRSLHFVGAVLDVAIDWSESAPTLPFREFK
                     ASGYNVIRVPVPWSDLEPEPMNVSAETLLAVRGFIDRAAAAGIAVILQNQVPFSDIGP
                     CQLNAGGPDWAFRVPVTGDPAQHGQCQDDGADANDAARTQASASIRADRFFSDFLYAD
                     WTPDGLTLQDHLIRSWTKLAEVAGRSAGLLGYQIVSTTPCPGDATAGCTRAWQEFLDR
                     FVQGVLAVADTSVFFIGSGGMDDRGRPDKTAGDRRAIAVLTEIVPSNADDYPGPYLSD
                     VNGVFGLKHVPVIDFSGFISTVAGIEKRGRSFLVTITDRVSAARFAKDGIDPFGRLMA
                     RPRPEQVSGIAPSWTFDRLRLPDGPGQMAEGPGTTDVFSLRFLTLPLREGLRSETTVR
                     LPVAHMYGEQDRDGSPPFTVDVSDGTVEWLPGKSDTIVWRTDDSLSEHSMKIVPWGGR
                     RLDFMTDLADIPAR"
     gene            6029..>6918
                     /gene="cysK"
                     /locus_tag="PKH54_05055"
     CDS             6029..>6918
                     /gene="cysK"
                     /locus_tag="PKH54_05055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOC99292.1"
                     /translation="MHEKPERILDLIGNTPMLRIPGFAMGDVFAKAEFLNAGGSVKDR
                     IAKHMLDCAEAEGKLKPGMTIIESTSGNTGIGITLCGVQKGYRVIIVMPENMSDERKK
                     IIRALGGELVLTSAEASISGSNDRLEELKAEIPNHFVPGQFTNPNNPDAHYTTTGPEI
                     WAQMEGKVDAFVAGVGSGGTLMGVGRFLREKNPRVQIIAVEPRNNSALLGKEPGLHTI
                     QGIGDGFVPGVVDRSYIDMVMTVSDEDAIETARRLAREQGILVGTSSGANVWVASHFA
                     DGRRNVVTILADRAERYFSTA"
BASE COUNT         1417 a         2053 c         2042 g         1406 t
ORIGIN      
        1 ggccggggct gtcggcgccg ccggagccgc aggggccggg gcttcagaag ttggggcggc
       61 cggggcggcc ttcgcgggtt ccgtcagcgg cttgtcgccc gccaccgcga ccacggtcga
      121 aaactcaagc acgcccatct tttccaggtc gtggaaatcc cttccgcctg gaccccacgc
      181 atgtgcgcgg cccagtcggc cctgatgcat gttcatgcgg aagaaattgg cgcgcagcgt
      241 ggtgccggga gatatcgacg ccagaccggg gatcgacttg acgggaatct tcacttccat
      301 cgtccagccg cgatcgatgt catcgacctt gttgatggtg ccgtcgagct tgaccgccga
      361 aaccagtcct tcaaccgtat gcttcgcggc gtcttcccac ttcggcgagc ggtgcgactc
      421 gaaaagcgcg tcgaaaacaa cgttggccgg cgaaacctga atctccaggt aattcttgcc
      481 atcaagaagg gcgtccagat agatttcgaa aacgtcctcg gtccaaagct gatcgtcgcg
      541 attcttcagc gtggcctcga tcgacaaatc cgttgcagag atcccgaagt aaatgaaatc
      601 attgtcgttc atgaccatga ccgaagtacc ttcctcgccg gaaatcggct ttccgaaggc
      661 gtcgccaagc cgaacgatcc ttgccgcacc cttccaggct tcctcgtcaa gcgcgccatc
      721 gataacgggc gccgagccaa tcacgggatt gaccgtaagt ctggtgtccg gggccggggg
      781 cgtcgccgaa cggttcatct gaaattcgac gacattgacc cggttttcgc cgtcgtgctt
      841 cacttgatcg cgattgacga caatcatgcg ctcgccggtg ccgcgtgaat tgtaaagatc
      901 cacgctgaac agcccggccc acatgaccgc cctgcccact tcctgcttgg cgtcgatctt
      961 gaagttcatc tggtacttca cgtactggcc gggctcccac ctgtccatcg ggaacagttc
     1021 gcccaccggg acgtgatcca ggcccatgcg cttgcccttc ggaagctcga gatggacgaa
     1081 gaccttccag ccgtcctgaa ccttgcgggt gcaatgccag taccaggtaa ccgtgacttc
     1141 ctgaccggga gccggattgg ccggcttcat atcgacgccg atcagctcga ccttgtcgcc
     1201 gaaattggcg ttgacgacat gctcgggcgt cggggccgcg tccaggatgg attcctggat
     1261 gcgtgcctgc tgatccttgg aaagtacaag cctggccggc ctgcgacatg caccggcgac
     1321 cgaaatcacc gcgaccacga ccgccagcga aagtaaaacc cttgaaatcc ggataccttt
     1381 catattttcc tcttcagccc gaaaggggcc gttgaaacat ccgcaatcaa tcctggttcg
     1441 gctcgatgta cgtgtattca tcgccacgga aattgcgtac gacaatcacg ccattctccc
     1501 aggacttcac gtactccgga agaatcggaa ccttgccgcc gccgcccggc gcgtcgatga
     1561 cgtagtacgg aacggccagc ccacttgtaa agccgcgcag cgacttgatt atctcgattc
     1621 ccttcgaaac cggcgtacgg aaatgatagt tgccgaaagt cggatcgcac tggtagatgt
     1681 agtacggctt gacctgcatc atcaacatcg cgtgattcaa cttcatgacg gtttcggcgt
     1741 cgtcgttgat acccttcaga agaaccatct ggttgccgat aggaatcccg gccccggcaa
     1801 tctttcgaag cgcggcaaac gcctcggcgg tcgcctcctt cgggtgattg aaatgggtat
     1861 tcaggaaaat cgggtggtac ttcttcagca tcgcgacgag ttcatccgtg atgcgcatgg
     1921 gcatcgtgca cggcgtcctg gtggctatgc ggatgatttc cacatggggg atatcccgaa
     1981 tctgggccag gatcttttcc aggcgatcgt ccgacaccgc caatgcatcc ccgccggaaa
     2041 cgatgacgtc gcgaacgcat ggagtccgcc ggatgtaatc gatgcacccc tgaatctggg
     2101 cgtcgtcggc ggccgtgtgg gggttggcaa ccttgcgctt gcgggtgcag tggcggcaga
     2161 acatcgcgca atcctgggtc atgtagaaaa gcacgcgatc cggataccga tgggtcagac
     2221 ccttgacggg catgtcgcga tcttccgcga gcggatcttc catgtcggat ggatgcgaaa
     2281 tcatttcctg tggatgcggg gttgcctgca tgcgaaccgg gcacagcgga tcatcccgat
     2341 ccatcagggc cacgtaatac ggggtgatcc caatcttgaa gacgtcccgg ccggcttcat
     2401 gaccggcgat ttcttccgga ctcaggttga tgtatcgacc gagttcggcg atatcggtga
     2461 tgcgatgctg caactgccat ttccagtcat accactgagc atcgggaaca tcgtggaacg
     2521 ggccctttcc aacgggcttg aaccttgccg cgatcgtctt cggcacatcc ttgaaatccg
     2581 gactgtaacc agacatctgg aactcctgaa aaagaagggc cgcgggcaat caaccaggcg
     2641 gccggaagtt gttgggtgca attatattca acttcgctct ttattaaagg cgcgcctgcg
     2701 cgaatggggc gatttcgaca gcaaagttca gcttgctcct gcatgccgca cgatttgttt
     2761 gaattgatcc ccgtatcggc ggtaaccttg gctgcctctt gatggagttt ctggcaatgg
     2821 cagtaaagga ataccagttc aatcacccgt acgacgccca gaccgccatg gacaagattc
     2881 gcccgcttct tggcaagtgg agtggcgcct acaagcttga catggaacag accagcccga
     2941 cgcagtacac gctttcgacg accggcgtcc aggccgaaat caacatcgcg gacaacaagg
     3001 ccaccgccgt ggtcgaaatg aacatgatga tggaaatgat gttccgcgga aagattgaag
     3061 aggcccttga acagaagctg cgtcccgcaa tcgaaaagat gtgacgtcag tttgaagccg
     3121 ccacgtcccc ggtgttggaa tgcggctttg agtcgtcctt tcccttgccg gtgagttcga
     3181 acatccgttc gatggctttc aatgccccca gcctgacttg ttcgtcgatc tcgaccacct
     3241 gatccggcct tggtgactca agaacctgca gaacctggtc aagcccgatc atcttcatgt
     3301 gcggacaaac ggtgccaagc gcgaagaagc gatattcggg gaattcaatc ctgaacctgt
     3361 cggtcatccc gtcttccgtg atgacaacga acgtgttcca gcgacccgac ttgacggcct
     3421 tcatcatgtc accggttcca ccggcgacat cggccatcgc gacgatctcg ggcggcgact
     3481 ccgtgtgaac catgacacac gcgccgggcc ccgccttttc acgaagtgcg gccacatgct
     3541 ccgggcgtat gcagtcatgg acgatgcaat tcccggtgta ccccttgatt gtcttggccg
     3601 tcatgggcgc gaggttggcc gccatcctgc cgtcgggcac gaaaaatatc ctggaggacg
     3661 gggcagcctc gacaacggca agggcgttcg cggatgtaca gcaggcatcg cattcggcct
     3721 tgaccgcggc agaggtgttc acataacaga cgatgctgtg gtccgggttt gcgtgcctga
     3781 agcggcgcac gtcttccgcg gtaatagatt cgcttaacga acatccggca ttcggggccg
     3841 gcagaagaac cgtacgcgac ggggcaatga ttttggccgt ttcgcccatg aatcgaacgc
     3901 cgcagaacac aatcacgtcg gcgtccgtgt cgcgtgctat cacggacaga ccaagggaat
     3961 caccacgatg atcggcaacc ccgaagatga cctccggagc gacgtaggaa tggcccagga
     4021 tcacggcccg cttctgccgg gcaagttcac gaattcgaag tgtttttggc gcaattccgg
     4081 cacactcggg gacggaccac ccgacatgct tcaagaatcc gtgcagccgt tcggtttcgg
     4141 cctgaatatc gacggcggaa taatcagaat tcacaacacc ccccaaacgc gcgatttccg
     4201 ggatcgtcgg gaaacggcgg aaactgcgac gaatccaccc ttgcggcgcg ggcctgcgcg
     4261 gaagcttccg accgcgcgtc cgcgggctgt ttcaaagccg ccgacgcccc ttgaagatag
     4321 cgacccgccc gcatcacgtc aacgacgata gtaattggat ggcgggccga tgcgctgata
     4381 tgctacacag gtgctgccgg ggcaagatgt ttcgtggggg atcggatgaa gcggcgaaca
     4441 aggatttttg cgatcgttct ggtcgtgctt cacctggccg gtttcggcat tttcttcgcc
     4501 ctgcggccgc aatccggacc ggccattccg ccggaccgca aggttgactg ggtattgcca
     4561 cccggccagt tccagcataa gtgcggacga gtctgcttcg acacggagcc gggcaaccgc
     4621 gggggtttca aggatccgga cggccgctcg ctgcactttg tcggggcagt ccttgacgtc
     4681 gcgattgact ggtccgagtc cgcgccgacc ctgccgttca gggaattcaa ggccagcggc
     4741 tacaacgtta tccgggtacc ggttccgtgg tcggatctgg agcccgagcc gatgaacgta
     4801 tcggcggaaa cgctgctggc tgtccgtggt ttcattgacc gtgccgcggc ggccggtatc
     4861 gcggtcatac ttcagaatca ggttcctttt tccgacatcg gcccatgcca attgaacgcc
     4921 ggcggcccgg actgggcctt ccgcgttcct gtaaccggcg acccggcgca acacggtcaa
     4981 tgccaggatg acggtgctga cgccaatgat gccgccagga cacaggcttc cgcgtcgatc
     5041 cgggcggatc gattcttcag cgattttctt tatgccgact ggactccgga cggactgacg
     5101 ttgcaggatc acctgatacg ttcatggacc aagctggccg aggttgccgg cagatccgcg
     5161 ggattgctgg gatatcagat tgtctcgaca acgccgtgtc ccggtgacgc gacggccgga
     5221 tgcacccggg cttggcagga attcctggat cggtttgttc aaggcgtcct ggcggttgcc
     5281 gataccagcg tattcttcat cggatccggt ggcatggacg accggggccg ccccgataaa
     5341 accgccggcg atcggcgcgc gattgccgtt cttaccgaga tcgtgccgtc aaatgccgac
     5401 gactaccctg ggccgtacct gtctgacgtg aatggcgtct ttggtctgaa gcatgttccg
     5461 gtgatcgatt tttccggttt catttcgact gtggccggga ttgaaaagcg cgggcgatca
     5521 ttcctggtga cgataacgga tcgggtttcg gcggccaggt tcgcgaaaga cggtatcgac
     5581 ccgtttgggc gcctgatggc gcggccacgc ccggaacagg tatcgggaat cgcgccatcg
     5641 tggacgttcg atcgtttacg ccttcctgac ggaccgggac agatggcgga aggacccgga
     5701 accactgatg tgttcagtct tcgcttcctg accttacccc ttcgggaggg ccttcgatcc
     5761 gaaacgacgg ttcgcctgcc ggtggcccac atgtacggcg aacaggatcg tgacggctcc
     5821 ccgcctttca ccgtcgatgt atccgacggc accgtcgagt ggctgccggg aaagagcgac
     5881 accatcgtgt ggcgcactga cgattccctg tccgagcact cgatgaaaat tgtgccatgg
     5941 ggcggcaggc ggcttgattt tatgacggat ttggcggata ttcccgcgcg ttgagacttt
     6001 acgcgtcacc aagacaggac ttgccacgat gcacgaaaaa cctgaaagaa ttcttgatct
     6061 gatcggcaac acaccgatgc ttcgcatccc gggatttgcg atgggcgatg tattcgccaa
     6121 ggccgaattc cttaacgccg ggggcagcgt caaggatcgt atcgcaaaac acatgctgga
     6181 ttgtgccgag gccgaaggca agttgaagcc cggcatgacc atcatcgaat cgacgtccgg
     6241 caacacgggg atcggtatta cgctgtgcgg cgttcagaaa ggttaccggg tcatcatcgt
     6301 gatgccggaa aacatgagcg acgaacgaaa gaaaatcatc cgtgcgcttg gcggcgaact
     6361 ggttctgact tcggccgagg cgagcatcag cggaagtaac gatcggctgg aagaactgaa
     6421 ggctgaaatt ccaaaccact tcgtccccgg acagttcacc aatccaaaca acccggacgc
     6481 tcactacacg acgaccggac cggaaatctg ggcacagatg gaaggcaagg tcgatgcgtt
     6541 cgtcgctggt gtcggcagcg gcggaaccct gatgggagtt ggccggtttc ttcgtgaaaa
     6601 gaacccgcgc gtccagatta tcgcggtcga gccgcgcaac aactcggccc tgcttggcaa
     6661 ggaaccgggg ctgcacacca tccagggaat cggcgacgga ttcgttcccg gtgttgtcga
     6721 tcgcagctat atcgacatgg tcatgaccgt ttccgacgaa gacgccatcg aaaccgcaag
     6781 gcgcctggcc cgcgagcagg gaatcctggt cggaacctcg tccggcgcga atgtgtgggt
     6841 ggccagccac ttcgccgacg gacgccgcaa cgtggtcacc atcctggccg accgcgccga
     6901 acgctacttc agcaccgc
//
LOCUS       DAOPPA010000118         6912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12095_length_6912_cov_4.217588, whole genome shotgun sequence.
ACCESSION   DAOPPA010000118 DAOPPA010000000
VERSION     DAOPPA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12095_length_6912_cov_4.217588"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PKH54_05060"
     CDS             <1..1405
                     /locus_tag="PKH54_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99293.1"
                     /translation="VSYDLPEGVEPPTSVIAIQSADTSTGCTTNTSIKTIADGLAFND
                     LVVVSPKFVAKAGQLDGYYVCQGLLNPLLPYSCIAMTIDVSAPEKFAPGDVIDAEGKV
                     IEYYCFTEISATAATKVGTAEIPDPIELAPELPGSQNPSQVEPYEGVLVTVSNVEITE
                     TPTNGSDGKDHGSFKVTGGLVVGNAFYLEYMNSRSDGRAIGQKFLSITGVLAYSYGRY
                     ELMPRSMADLEFDGDPIVVEKDQPDIVAPDVPDAPLTMHQIQAATPSTGCTTDGFVNV
                     FNNVAFAQVIVTAPKFSSTSSLDGYYVEDYPVAEDRSFNGMAVVADKTLATAFVPGDV
                     ITISDGDYYEYYCFSQVQINAAAKDSTDTAPGPIDIDLSVLENAGSGALARCEELEGL
                     LVRIPATTITAATSSDTKTWFEVGNGIHVSNQFYIQGFTPTADAAVASITGLVRYHFG
                     KFRIAPRSLDDIVMATK"
     gene            1565..2641
                     /locus_tag="PKH54_05065"
     CDS             1565..2641
                     /locus_tag="PKH54_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99294.1"
                     /translation="MLKRIPLLLCALVAASLTAVSLDAVAQSAAEDAGWQEEKPAEEP
                     AAADDSEQAAATDGEKAEKKAEEPFDEDKPMVRGKTVYMADRGIILPLGTGEARLDLF
                     VGLNKGMEGRVFGVGSALASERNVGLTFRGTLWGPFEMGMSVQYLQTLDNGKKTRPHG
                     NFDGTTTPPSTGRGPEGDGTRRVAGYGYKFADGFSQFVPAYLYGRFKVVEQFGVELGL
                     QIPTMQADGFNRPALRIGLPVQYIAVPGLLKIHARPDLLIGFGTTEPQDSAMPSEPVN
                     VGFYVDAGITISVAKVFFDVTVGYGLDVYPFKSGYLPMSILVGYQVLTRLDVFAGFTL
                     ENMTPEFGGAGDARSLSVGFQCRF"
     gene            2678..3568
                     /locus_tag="PKH54_05070"
     CDS             2678..3568
                     /locus_tag="PKH54_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99295.1"
                     /translation="MTLARLAAAVLIVFPAATVLAETVVPIVDRPIVCPEGQGSLTFD
                     MTVGLNATDPGRTGGVYSDLASETRGGLGASYGFAKGIEAGVVLPWLFLDMDGDTVQS
                     FNVAMAGWPMFSNTNSRSHFRQVFAFGKFRLADFVAVELTLAIPLEEIKANQVGARAG
                     LPFKFAVLPGRLAVHFRPELRLGFANKDRAFGNPVQITAWFDAGLTVNATKSLYFDVS
                     LGYGRTFLPSPAKMVTAEYMGAPGYGASGWLPLSVTMGYTIIESIDLFVGFTMTNLAP
                     EGSIAPSDGRSLTIGLDYRF"
     gene            3580..4203
                     /locus_tag="PKH54_05075"
     CDS             3580..4203
                     /locus_tag="PKH54_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99296.1"
                     /translation="MNRLLSVLAVCLLIPAAAGARDMNQKFGIGYEQSVGGVSGINLK
                     YHIKKFTIAGTVGFDIFKPTDSDPRSAVRFAAGAFYDFASFKKVNMGIGLRVNAGWKN
                     AEAVNAGRRKELGCTDNDSCSGMVKSGDVWQVNLEIPLVVEVFLSDHFAFTLSAGFVI
                     TILTEDEVVLGTDRLNPSMASGTAESGFGFSLGMGSMLATAGFMVYF"
     gene            4240..6351
                     /locus_tag="PKH54_05080"
     CDS             4240..6351
                     /locus_tag="PKH54_05080"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOC99297.1"
                     /translation="MTPPALLKAVILAALMGAVACGGDPRTDTGDLTPDIPTDIGTDI
                     PDIDQEFEVCRPGTIQGCSTDGFGEVVCARDGSGWEVVSCGQGSLCVAEKGRCSSCIP
                     GTLRCLNDDIIQRCDDSGTKWEDHQDCNGAETGKVCRLGVCIGLCELNSKLSSYIGCE
                     YWAVDLDNAFVPSNSESGYADAAGSPWAIVVSNTSTKYPARVEIDCKYGPVTRDHWGN
                     ELDFSPIPPMSLRIFTIARKYSVVDPASQYFGQEFPMDLDGTSIKPQAYRVKSSIPIT
                     AYQFNPLENEEVFSNDASILIPSNSLGRYHLVMTREQTFNDLKGFLTVVAINSGETQV
                     NVTVTAPTLGGDGVPAMTGGTGKTFILNQWDVLNIETNAYGADLTGSIVLANHPVAVF
                     GGSEAANAPNTNHCCPDGKCRYHQEWLECASRNDCLCEWPANNLDPPRDVTCRNNYDC
                     IGYNTCCADHLQMQMFPVTTWGTKYVATLSYPRGSERDVWRIMAASDNTQLTTYPSQT
                     DNWVINRGQFVEFEADENFEIIGKKPLLVGQFLAAQQAPDPGSGETDAKTGDPTFILA
                     VPVEQFRTEYVFLAPDKYMFDCVNIIAKPGVPVFLDGQELRPEDLTFRTIRKMQSEMT
                     EKGYEHPSELGIRFGDYKIVGQGDWAVFRLVIEDGVHTASSAEPFGVISYGYDQYVSY
                     GYPAGLNLQDLKLIDEEPIGD"
     gene            6357..>6912
                     /locus_tag="PKH54_05085"
     CDS             6357..>6912
                     /locus_tag="PKH54_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99298.1"
                     /translation="MAQRKKFARIRLLAASLALVACIHLPGRARADFGDIRNVFSGTA
                     WTLQEGDFVVGIIGPMTFGLVDQFTLTTHPILDLLLIPNVNLKWKFFDNDKVALALDI
                     AYIQSFLDTELTDIPGSLSPYMILSVPIKDVAALTFRAGYVLELSPNSHGLTYGAEVS
                     WLVTAADLISVGVSGQYHPRGRGAD"
BASE COUNT         1430 a         2061 c         2058 g         1363 t
ORIGIN      
        1 cgtttcatac gaccttccgg aaggcgtcga gccgccgaca tcggtcatag cgatacagtc
       61 ggcggacacc agcaccggat gcacgaccaa cacatccatt aagaccatcg ccgacggcct
      121 cgcattcaac gacctggtcg tggtaagtcc gaaattcgtc gcgaaggccg gccagcttga
      181 cggctactac gtctgccagg gcttgctgaa tccgcttctg ccctactcgt gcatcgcgat
      241 gaccatcgac gtgtcggcac ccgaaaaatt cgcgccgggc gacgtcatcg acgcggaagg
      301 gaaggtcatc gagtattact gcttcaccga aatttccgcc accgcggcga cgaaggtcgg
      361 caccgccgaa attcccgacc cgatcgaact ggctccggaa ttgcctggct cccagaatcc
      421 atctcaagta gaaccatacg aaggcgtcct ggttaccgtt tccaatgttg aaatcaccga
      481 gaccccgaca aacggatctg acgggaagga tcacggttca ttcaaggtaa ccggcggatt
      541 ggtggtcggg aatgccttct acctggagta tatgaactcg cgttccgatg gaagggcaat
      601 cggacaaaag ttcctgtcga tcaccggcgt gctggcatat tcgtacggaa ggtatgaact
      661 gatgccaagg tcgatggccg acctggagtt cgacggcgac ccaatcgtgg tcgaaaagga
      721 tcagcccgac atcgtggcgc cggacgtgcc ggacgcgccg ctgacgatgc accagatcca
      781 ggcggcgacg ccgtcaaccg gctgcaccac agacggcttc gtcaacgttt tcaacaatgt
      841 cgcgttcgcc caggtgattg ttacggcgcc gaaattctcc tcgacgtcca gccttgacgg
      901 atactatgtc gaagactatc cggtcgcgga ggatcgttcc ttcaacggaa tggccgtcgt
      961 ggcggacaag accctggcaa cggccttcgt gcccggcgac gtcatcacga tttccgacgg
     1021 cgactactac gagtactact gcttcagcca ggtgcagatc aacgcggccg caaaggattc
     1081 gaccgacacg gccccggggc cgatcgacat cgacctgtcg gtgcttgaaa acgccggaag
     1141 cggtgccctg gcgcgctgcg aggaacttga aggtcttctg gtccggatac cggccacgac
     1201 aatcactgcc gcgacgtctt cggataccaa gacctggttt gaagtcggaa acggcatcca
     1261 tgtcagcaac cagttctaca tccagggatt taccccgacg gcggacgccg cggttgcttc
     1321 gattaccggg ctggttcgct atcacttcgg caaattccgc attgctccca gaagcctcga
     1381 tgacatcgtg atggccacga aataggcttt ttcctgacag cggccggcat cggccgaaaa
     1441 aatcccgaaa ttgcgccgat taccgtcatt taccttgtgc cattttaagt ggttcctgtg
     1501 atatgcttgc cgcgactgtt taccgggtcg cccggttttg aattcggtaa gaccggagga
     1561 agtgatgctg aagagaattc ccttgctcct gtgcgccctg gtggcggcgt cgctgacggc
     1621 cgtcagcctt gatgcggtgg cgcagtccgc ggccgaggat gccggctggc aggaagaaaa
     1681 gccggcggaa gaaccggcgg cggccgacga ttccgaacag gcagccgcga ccgacgggga
     1741 aaaggcagaa aagaaggccg aagaaccatt cgacgaagac aagccaatgg ttcggggcaa
     1801 gaccgtctat atggcagacc gcggaatcat cctgccgctc ggcaccggcg aggcgcgcct
     1861 ggatctgttc gtcgggctta acaaaggcat ggaaggtcga gttttcgggg tcggctcggc
     1921 gctggcttcg gaaagaaacg tcggcctgac cttcagggga accctgtggg gcccgtttga
     1981 gatgggcatg tcggttcagt atctgcagac gctcgacaac ggcaagaaga cccggccgca
     2041 cggcaacttc gacggaacca ccaccccacc ttcgaccggc cgcggccccg aaggcgatgg
     2101 aacccgcagg gtggccggtt acggatacaa gttcgccgac ggcttcagcc agttcgttcc
     2161 cgcctacctt tacggtcgtt tcaaggtcgt ggaacagttc ggcgttgaac tgggccttca
     2221 gattccgacc atgcaggccg acggattcaa ccgcccggcc ctgaggatcg ggctgccggt
     2281 tcagtacatc gcggtgccgg ggctgctgaa gatccacgcc aggccggatc tgctgatcgg
     2341 ttttgggacg accgagccgc aggattccgc aatgccttcg gagccggtca acgtcggatt
     2401 ctatgtcgat gccggcataa cgatctcggt tgccaaggtc tttttcgacg taaccgtcgg
     2461 atacggtctg gacgtttatc cgttcaaatc cggttatctg ccgatgtcga tcctggttgg
     2521 ttaccaggtt cttaccaggc ttgacgtttt cgccgggttc acgcttgaaa acatgacccc
     2581 cgaattcggc ggcgccggcg acgcccgcag cctttctgtc ggcttccagt gccggttcta
     2641 ggccgcagtg gccgtttttc tccatgcgga tttcccgatg actcttgcaa gactggcagc
     2701 ggcggtattg attgttttcc cggcggcaac cgtgctggcg gaaacggtcg tgccgattgt
     2761 tgatcgcccg atcgtatgcc cggagggcca gggttcgctg accttcgaca tgactgtcgg
     2821 ccttaacgcc accgatcccg gacggacggg cggtgtctac agcgacctgg ccagcgagac
     2881 ccgggggggc cttggggctt cgtacggatt tgcaaaagga atcgaggccg gggtcgtcct
     2941 gccgtggctc ttcctcgaca tggacgggga caccgtgcag tcattcaacg tcgccatggc
     3001 cggatggccg atgttttcga acacgaacag cagaagccac ttccgccagg tcttcgcttt
     3061 cgggaaattc agacttgccg atttcgtcgc cgtcgagttg acccttgcga taccgcttga
     3121 ggaaatcaag gccaaccagg tcggggccag ggccggactt cccttcaagt tcgcggttct
     3181 gccaggcagg cttgccgtcc acttcaggcc ggaactgcgg cttgggttcg cgaacaagga
     3241 tcgcgccttc ggcaaccccg tccagatcac ggcatggttc gatgcgggac ttacggtcaa
     3301 cgccaccaaa tcactctatt tcgatgtatc gctgggatac ggcaggacat tccttccatc
     3361 gcccgcgaaa atggtcactg ccgaatacat gggggcgccg ggctacggcg cttccggatg
     3421 gcttcctctt tccgtcacga tgggttacac catcatcgaa tcgatcgacc tgtttgtcgg
     3481 attcacgatg acaaaccttg ccccggaagg atcaatcgcc ccgtcggacg gaagatcgct
     3541 tacaatcgga ctcgactaca gattctagag gggccctgaa tgaacaggct gctttcggtt
     3601 ctggccgtct gcctgctgat tcccgcagcg gccggcgcgc gcgacatgaa ccagaagttc
     3661 gggatcggtt acgaacagag cgtcggcggc gtttccggga tcaaccttaa ataccatatc
     3721 aaaaagttca cgatcgccgg gacggtcgga ttcgacatct tcaagccgac agattcggat
     3781 cccaggagcg cggtcaggtt cgccgccggg gccttctatg acttcgcgtc gttcaagaag
     3841 gtcaacatgg gcatcgggct tcgcgtcaac gcgggctgga agaacgccga ggcggtcaat
     3901 gccggccgac gaaaggaact gggctgcacc gacaacgatt cgtgcagcgg catggtcaag
     3961 tccggcgacg tctggcaggt gaacctggaa attccgctgg tggtggaagt gttcctgtct
     4021 gatcacttcg cattcacatt gtccgccggt ttcgttatca ctattcttac cgaggacgaa
     4081 gtcgtgctcg gcaccgatcg gctgaatcca tcgatggctt ccggcaccgc cgaatccggc
     4141 ttcgggttca gcctgggaat gggaagcatg ctggccacgg ccggtttcat ggtttacttc
     4201 tgatgccggg cgtctggccg cattgaagga aggtgcccga tgactccacc agcactgctc
     4261 aaggccgtca tcctggccgc cctgatgggc gccgttgctt gtggcggcga cccgcggacc
     4321 gacacgggcg acctgacgcc ggatatcccc accgacatcg gaaccgacat cccggacatc
     4381 gaccaggaat tcgaagtatg ccggcccgga acaatccagg gatgttcgac agacggtttc
     4441 ggcgaggtgg tgtgcgccag ggacggatcc gggtgggaag tcgtttcctg cggccagggc
     4501 agcctctgcg tggccgagaa ggggcgctgc tcgagctgca ttccgggaac gctgcgatgc
     4561 ctcaacgacg acatcataca gcgctgcgac gattcgggca ccaagtggga agatcatcag
     4621 gactgcaacg gcgctgaaac gggcaaggtg tgcaggcttg gcgtctgcat cggcctttgc
     4681 gagctgaact cgaagctgag ttcatacatc ggctgcgaat actgggcggt cgatctggac
     4741 aacgcctttg tccccagcaa cagcgaaagc ggctatgcgg acgcagccgg ttccccatgg
     4801 gcgatcgtcg tttcgaacac cagcacgaag tatccggcgc gggtcgaaat cgactgcaaa
     4861 tacggcccgg tcacccggga tcactgggga aacgaactgg acttttcgcc cattccgccg
     4921 atgagcctca ggattttcac catcgccagg aaatattccg tcgttgaccc cgccagccag
     4981 tatttcggcc aggagttccc gatggatctt gacgggacgt cgattaagcc gcaggcgtac
     5041 cgcgtcaagt catccatccc gataacggcc taccagttca acccgctgga aaacgaggaa
     5101 gtcttctcga acgacgcatc gatcctgatt ccgtcgaact cgctcgggcg ttaccacctg
     5161 gtcatgaccc gcgagcagac cttcaacgac cttaaggggt tcctgacggt cgtcgcgatt
     5221 aacagcgggg aaacccaggt aaacgtcacg gtgaccgcac cgacccttgg cggcgacggc
     5281 gttcccgcca tgaccggcgg aaccggcaag acgttcatcc tgaaccagtg ggacgtgctg
     5341 aacatcgaga caaacgcgta cggtgccgac ctgaccggtt caatcgtgct ggcgaaccac
     5401 ccggtcgccg tattcggtgg aagcgaggcg gccaacgccc cgaacaccaa ccactgctgt
     5461 cccgacggca agtgccgcta ccaccaggaa tggctggaat gcgcttcgcg caacgactgc
     5521 ctctgcgagt ggcctgcaaa caaccttgat ccgccgcgcg atgtcacctg ccgcaacaac
     5581 tacgactgca tcggctacaa cacgtgctgc gcggatcacc tgcagatgca gatgttcccg
     5641 gtcacgacat ggggcacgaa atacgtggcc acgctcagtt acccgcgcgg ttcggaacgc
     5701 gacgtctggc ggataatggc cgcgtccgac aacacccagt tgacgacata ccccagccag
     5761 actgataact gggtaataaa ccgcggccag ttcgtggagt tcgaggccga cgagaatttc
     5821 gaaatcatcg gcaagaagcc gctgcttgtc ggacagttcc tggcggccca gcaggctccc
     5881 gacccgggtt ccggcgaaac cgacgcgaaa acgggcgacc cgaccttcat ccttgcggtt
     5941 ccggttgaac agttccgaac cgaatacgtt ttccttgcgc cggacaagta catgttcgac
     6001 tgcgtcaaca tcatcgcgaa gccgggggtc ccggtctttc tggacggcca ggaactccgg
     6061 cccgaagacc tgaccttcag gacaatccgg aagatgcaat cggaaatgac cgaaaagggc
     6121 tatgaacatc cgtccgaact cggtatcagg tttggtgatt acaagattgt cggacaaggc
     6181 gactgggcgg tcttccgcct ggtcatcgag gacggagtcc acaccgcgtc ttctgccgaa
     6241 cctttcggcg tgatatcgta cggttacgat cagtacgtgt catacggata ccctgcggga
     6301 ctgaacctgc aggatctgaa actgatcgac gaggaaccga tcggggactg acctggatgg
     6361 cgcagcgaaa aaaattcgcc agaatcaggc tgctggccgc ctcgctggct ctggttgcgt
     6421 gtattcacct gccgggccgg gcaagggccg acttcggcga cataagaaac gtcttttcgg
     6481 gaacggcctg gacccttcag gaaggcgatt tcgtggtcgg catcatcggg ccgatgacgt
     6541 tcggccttgt tgaccagttt acgctgacga ctcacccgat cctggatctt ctgctgatcc
     6601 cgaacgtcaa tctgaaatgg aagttcttcg acaacgacaa ggtggccctg gctctggaca
     6661 ttgcctacat acaaagtttc ctggacacgg aactgacgga cataccgggt tcgctgagcc
     6721 cctacatgat tctttcggtt ccgatcaagg atgtcgcggc gctgactttc agggcgggat
     6781 atgtgcttga actcagcccg aattcacacg gactgactta cggggccgaa gtttcgtggc
     6841 tagtaacggc ggccgacctt atttccgtgg gagtttccgg gcagtaccat ccccgtggac
     6901 gcggcgccga tg
//
LOCUS       DAOPPA010000119         6886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12151_length_6886_cov_3.628458, whole genome shotgun sequence.
ACCESSION   DAOPPA010000119 DAOPPA010000000
VERSION     DAOPPA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_6886_cov_3.628458"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKH54_05090"
     CDS             complement(<1..815)
                     /locus_tag="PKH54_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOC99299.1"
                     /translation="MPAKVDSPFVFIDWWVMAGFSRRQFLKSIIAGGVGLASQLVVSE
                     LRAAGATTRRLLTSHADPARRIVIPDTPINLPPWNYGDSSSAADTVLMFRGSPARNFY
                     GTGPVGDRFHVRFKVEFGCFDTTLRGQPARWCGTGWTGQPAVLGGHVFVGGTDRCLYA
                     IEAQTGRVVWKTMFGRMIKSSVCVFRNRIYVGCVDDLFRCVDAATGQVVWSVHVGTDI
                     DSSACIWRGRLYVAGESGFLRCLDPEDGREIWRAFLGGVGPGSPPGSNGSEASP"
     gene            complement(796..3000)
                     /locus_tag="PKH54_05095"
     CDS             complement(796..3000)
                     /locus_tag="PKH54_05095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOC99300.1"
                     /translation="MKAKSVIRGIGYAAVIVSVIGCAGNQVREEQVSVNKPVDEAVSG
                     QPAPVVAERVNPLLVEWTTPFGVPPFDQILESDFVPAIKAGIQTQRAEIKAIVECSAE
                     PDFENTVLALDASGRLLEKVELVLYNLSSAETTPGIQAILKEIAPLTTALRDDIFLDK
                     VLFGRVDAVFRKAAALGLDPVQTRLVSEMRKSFVRAGAALDEAAQQRMRQINQELAVL
                     GLKFGDNLLAETNAFKLVLEKPEDLAGLAQSTIDAAAAAAAAEGQQGKWLITLSYPSM
                     WPFVQQSQRRDLRQQVIQAYASRCDRGNEYDNKEVLRRIAELRLEKAKILGYASWADF
                     VLEERMAKDAAGVDGLLDRLWKPALAVAAREAREYAAAARRDGLKGDFMPWDWHYYAE
                     RTRVAKYGLDAAELRQYFKLDNVLSGAFELARRLYGINFKPVEGLKLYHRDVLAWEVT
                     EADGTFVGLFLGDYHPRPGKRVGAWSSRYRSQEVVNGKDIRPIVVNVGNFTRPSGDDP
                     ALLSPDEAETLFHELGHGIHSLLAKVPYRFLGGVPRDFVELPSQIMENWVFEPEMLAL
                     YARHYKTGQPIPAELVAKIEAASRHNQGFATVEYLAASKLDMEWHRATKAEFGDAATF
                     EAAVLKRIGMPKEIIPRYRSVYFNHIFGGGGSYSAGYYSYIWSEVLDSDAFQAFKETG
                     DLFNPGVALKFRKNVLERGGTVEAGQMYRDFRGRDPAVEPLLKKRGLAGKGR"
     gene            3183..4142
                     /locus_tag="PKH54_05100"
     CDS             3183..4142
                     /locus_tag="PKH54_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99301.1"
                     /translation="MPKQTATELPSNVWIFQGNPDVFDLDAYLAEGSGRLTWEVNQNA
                     DRINDGDTVYLWRAVSRAVRARANALADKPFSRAARQARLAARPAIVAVARVVGSAFD
                     GPDDSDAVQFRHDLALPETRRRVWLDILANANGREAIRREWLASDPLCNSLPILDRCG
                     DTNIPVTTAQAERLDALWENSGRPMTRKDLLAILSCYCRVRAIESRSSGKALRESAGT
                     TRAAGKASAGPDRQSRLEIDQAIADTAVLVGRAIGGVKLAIAKFRALDPAYPGNGLSG
                     ATSVFHDVINQYLDSSRGCLDDAAVERDFANIWLGRSDSGLLL"
     gene            4336..4662
                     /locus_tag="PKH54_05105"
     CDS             4336..4662
                     /locus_tag="PKH54_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99302.1"
                     /translation="MTRQTKPAVSGSGENSLLGLLIRMYWMAFGNLPLLFLPILIIKS
                     RTPEFTRRDVMFIVAWAALAIARLADARFFNPVPAADVRKYILGLTGVSAGLIAFGHI
                     VAKLMS"
     gene            complement(4686..5750)
                     /locus_tag="PKH54_05110"
     CDS             complement(4686..5750)
                     /locus_tag="PKH54_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOC99303.1"
                     /translation="MNRFNTTFPLSLAICAGIVLIGVSAISCATSAAAKRPAWVDLGQ
                     HDKYPERRFMTGVGQGDTREKAADRARAEISKRFSVSVESRTVVVESTWIQDSNGAVS
                     QGSAGMVTDGVATSSSMQLEDLTIAETWFDKKTAHYYALAVLDRASTFDRVEKAIDGI
                     LKDLAAVMEQAESETDALRKAGRWATAMRVFSRLDPFLLQARVLNPNWTPEFPGGASE
                     GSLRRAFETAAKQVRVFVSVAPEDTKLAERVINAFRNLMTTAGMVCVSDESGATVRLQ
                     VELTARDSGKTPSGFEFTEIEAAVSIYLEGSAEVIASTTRKVREGGLNGKQARATAED
                     WIIKDISRGFLGFLYQSVLK"
     gene            complement(5784..>6886)
                     /locus_tag="PKH54_05115"
     CDS             complement(5784..>6886)
                     /locus_tag="PKH54_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99304.1"
                     /translation="ERNMVDLLHAMNFLSMGDYEAARCEAQQANTKLADFVELLRRNN
                     VTTGFVSDPFAYFMTGLAFEAAAAKEGGEQRFNLLDQARLAYTKALQDYQGVGGRYGV
                     EIPAGLPADVERIKAEVCNPQDMPASGTCKEGPSATEIIVVTGVGQIPHKESLKWIQS
                     DGQDNIVVTYPEFFPSPTAVEGAKVVMGGTDYVTSTVYDMGKVAVEVNKERLDQLKDK
                     AIARAMAKYVAKKAARAAMNAGNNYGGSGGGWAALIGGIVSASIQIAEAVEKADARSW
                     MSLPNRYTMARIPVEPGIHKFNVDFTNAGGMTVSTIPVEANVEPNSKTFVVLYSMDRP
                     GVAMTRKFGPGMEVEVPPPAAAQGEAPAETQM"
BASE COUNT         1392 a         2144 c         1923 g         1427 t
ORIGIN      
        1 ggggaagcct cggacccatt ggatcccggc ggggatccgg ggccgacgcc accaaggaac
       61 gcccgccaga tctcgcgtcc atcctccgga tccagacagc gcagaaaccc ggactcgcca
      121 gccacgtaaa gtcgaccgcg ccagatacag gctgaactgt cgatatcggt gccgacatga
      181 accgaccaca cgacctgacc ggttgccgcg tcaacgcacc ggaaaaggtc atcgacacaa
      241 ccgacataaa tcctgttcct gaaaacgcag accgagcttt tgatcatccg accgaacatc
      301 gtcttccaga caacccggcc tgtctgtgcc tcaatcgcgt aaagacatct gtcggtgcca
      361 ccgacaaata cgtgaccgcc cagaaccgcg ggctgtccgg tccagccggt tccgcaccac
      421 ctggccggct ggccgcgaag ggtcgtgtcg aaacaaccga attcaacctt gaaccgcaca
      481 tggaaccgat cgccgactgg cccggtacca taaaaattcc tggctggact tccgcgaaac
      541 atcagaaccg tatcggccgc cgaactcgag tccccataat tccatggcgg aaggttgatc
      601 ggggtgtctg gaatgacaat tcgccgggct ggatctgcgt gggatgtcag aagtcgccgg
      661 gtggtcgcac cggccgcccg cagttcactg acaaccaact gactggccag gccgacgcca
      721 ccggcgataa tggacttcag gaactggcgt cttgaaaacc ccgccattac ccaccaatcg
      781 atgaagacga acggactatc gacctttgcc ggcaaggccg cgcttcttca gaagcggctc
      841 aacggccgga tcgcgtcccc tgaaatcacg atacatctgt cccgcctcta ccgtgccgcc
      901 acgttcaagt acattcttcc tgaacttcag cgccaccccg gggttaaaca gatccccggt
      961 ttccttgaat gcctggaatg cgtccgaatc cagcacttcg gaccagatgt agctgtaata
     1021 gcctgccgag tagctgccac cgcccccgaa tatgtgattg aaatagacgc ttctgtaccg
     1081 cgggataatc tcctttggca tcccaatccg cttcagcacg gccgcctcga acgttgcggc
     1141 atcgccgaat tcggccttgg ttgccctgtg ccattccatg tccagcttgg atgcggccag
     1201 gtattcaacc gtggcgaacc cttgattgtg acggctggct gcttcgatct tggcgaccag
     1261 ttcagccggg atgggctggc ccgtcttata gtgacgggcg tacagcgcaa gcatttccgg
     1321 ctcaaaaacc cagttttcca ttatctgcga aggcagttcc acaaagtcgc gcggcacacc
     1381 gccaaggaac ctgtagggaa cctttgcaag cagcgaatgg ataccatgcc caagctcatg
     1441 gaaaagcgtt tcggcctcgt cgggcgaaag aagtgccgga tcatcgccgg acggccgggt
     1501 gaaatttccg acgttgacaa cgattggacg gatatctttt ccgttgacga cttcctgcga
     1561 acgatagcga cttgaccacg cgccaacgcg tttgccggga cgaggatggt aatcgcccag
     1621 gaacagcccg acaaaggtac cgtcggcctc ggtgacttcc caggcaagca cgtcgcggtg
     1681 ataaagtttc aagccttcaa ccggcttgaa attgattccg taaagccttc gagccagttc
     1741 gaaagcgccg gaaagaacat tatccaactt gaagtattga cggagttcgg cggcatcgag
     1801 tccgtatttg gcgacccgtg tccgctcggc gtaataatgc cagtcccacg gcatgaaatc
     1861 gcccttgagc ccgtccctgc gggcggcagc cgcgtactcg cgcgcctcgc gggcggcaac
     1921 cgcgagggcc ggcttccaga gcctgtccag aagaccatcc accccggcgg catccttggc
     1981 catcctctcc tcgaggacga agtccgccca cgacgcgtac ccaaggattt tcgccttttc
     2041 gagcctgagt tcggcgattc ttctcagaac ttccttgttg tcgtactcat tgccacgatc
     2101 gcatctggaa gcataggcct ggatgacctg ctgcctcaaa tcgcgtcgct gggactgttg
     2161 cacgaagggc cacatgctcg gatatgacaa ggtgataagc cactttccct gctggccttc
     2221 cgcggcggcg gccgcagcgg cggcgtcgat cgttgactgc gcaagccccg ccagatcctc
     2281 gggcttctca agcacgagct tgaaagcatt tgtttccgca agcagattgt cgccaaactt
     2341 caatccaaga accgcaagct cctggttaat ctgccgcatc ctctgctggg cggcttcatc
     2401 aagggcggca ccggccctga caaacgattt gcgcatttct gaaacaaggc gggtctgaac
     2461 aggatcaagc ccaagcgcgg ccgccttcct gaacaccgca tcaacccgtc cgaacagcac
     2521 cttgtcaagg aagatgtcat cccgaagcgc ggtggtaagc ggcgcgatct ccttcaggat
     2581 ggcctggatc ccgggcgtgg tttcggccga ggacaggttg taaaggacaa gctcaacctt
     2641 ttccagcagg cgtccgctcg catcaagcgc aagaaccgtg ttctcgaaat ccggctcggc
     2701 cgagcactca acgatagcct tgatctccgc tcgctgcgtc tggattccgg ccttgatcgc
     2761 cgggacgaag tccgactcca gaatctggtc gaatggggga accccgaacg gggtggtcca
     2821 ttccacaagc agcgggttga cccgttccgc cacaaccggg gccggctgcc ccgaaacggc
     2881 ttcatccaca ggtttattga cgctcacctg ctcctccctg acctgattgc ccgcgcagcc
     2941 gattacagaa acaatgacgg cagcgtatcc gattcccctg attactgatt tcgccttcat
     3001 caaaaccctt ccactgaata attccggtaa tcaagtaacc cgatttgcaa cacagcgtca
     3061 atcaggaatc ccgcaatttt tgaacaaatc agattgattt cggtcgaacg gcacaatccg
     3121 atcgattcaa agctccgggc agcctgtgct agaatttacc gtcattcgtg aaggaatcca
     3181 ggatgccgaa acagactgcg accgagttgc cctcaaatgt ctggattttt cagggtaatc
     3241 ccgatgtctt cgaccttgat gcgtatctgg ccgaaggatc tggcaggctg acgtgggagg
     3301 tgaaccagaa cgccgaccgc attaatgacg gggacaccgt ctatctttgg cgcgctgttt
     3361 caagagctgt gcgggcacgg gcgaacgcgc ttgcggacaa gcctttcagc cgggccgcgc
     3421 gacaggccag gcttgctgcc cgtccagcca ttgttgcggt tgccagggtc gtcggttctg
     3481 ctttcgatgg cccggacgat tctgatgccg tccagttccg gcatgatctt gcgcttccgg
     3541 aaaccaggcg acgcgtctgg ctggacattt tggcaaatgc gaacggacgc gaggcgatcc
     3601 gtcgcgagtg gcttgcttcc gacccgctgt gcaattcgct tccgattctg gatcgctgcg
     3661 gcgacacgaa tatcccggtt acaacggctc aagcagaaag gctcgatgcg ctgtgggaga
     3721 actccgggcg cccaatgacg cgaaaggatt tgctggcaat tctttcctgc tactgccgcg
     3781 ttcgcgcgat tgagtcgcga tcttcgggca aggccttgcg tgagtccgcc ggcacgacgc
     3841 gggcagccgg caaggcttcc gccggcccgg atcgtcagtc ccgtcttgaa atcgaccagg
     3901 cgattgccga tacggccgta ttggtgggcc gcgccatcgg tggcgttaag cttgcgattg
     3961 caaaattccg ggcgcttgat ccggcctatc caggcaacgg actgtccggc gccacttccg
     4021 tctttcatga cgtcatcaat cagtatctgg actcttcccg cgggtgcctt gacgacgccg
     4081 cggttgaacg ggattttgcc aatatctggc ttggccgctc ggacagcggc ctgttgctct
     4141 gacccggcgc ctggtggcaa atttatcact cggccttgac acaagccgct cccgcaacta
     4201 ctttacacgt ggtttgccgg tcgctccggc attttcacaa tgggtgcgcc gccacatccc
     4261 ggttggcgca ggtttataaa tcggtacggt cgggccgccg tccagagtca acaatgactg
     4321 gaaagggggc ctatcatgac cagacaaacg aagcctgccg tatcgggatc cggtgaaaac
     4381 agcctgctcg gacttctgat caggatgtac tggatggcgt ttggaaacct gccgctgctg
     4441 ttcctgccaa tcctgataat caagagccgc accccggaat tcacccgaag ggacgtcatg
     4501 ttcatcgttg cgtgggcagc cctggcaatt gcccgactgg cggacgccag gttcttcaac
     4561 cccgttcccg cggccgatgt caggaagtac atacttggtc tcacaggggt ttccgccggc
     4621 ttgatcgcgt tcggccacat cgtcgccaag ctgatgtcct gaactgtctg gattgatttg
     4681 aagaattact tcagcacgga ctggtagagg aagcccagga atccccggga aatgtccttg
     4741 atgatccagt cttccgccgt cgccctggcc tgttttccgt tgagaccgcc ttcccgaacc
     4801 ttgcgagtgg ttgacgcaat gacctcggca ctgccttcca ggtaaatgga aacggccgcc
     4861 tcgatctcag tgaattcgaa cccggacggc gtcttgccgc tgtcacgggc cgtcaattcg
     4921 acctgaaggc gaacggtggc ccccgattcg tcggacacgc acaccatccc ggcggtcgtc
     4981 atcaggtttc tgaaggcgtt tataacccgt tcggcaagct ttgtgtcctc cggggcgacg
     5041 cttacgaaaa cccgaacctg cttggcagca gtctcgaaag ccctccgaag acttccttcg
     5101 ctggccccgc cgggaaactc cggagtccag ttcggattca ggacgcgggc ctgcagcagg
     5161 aacggatcca gacgggaaaa caccctcatg gcagttgccc agcgaccggc cttgcgaaga
     5221 gcatccgtct cggactcggc ctgctccatg actgcggcca gatccttcag gattccgtca
     5281 atcgcctttt caacgcgatc aaaggtactg gcacgatcaa gaaccgcgag cgcgtaataa
     5341 tgagcggttt tcttgtcaaa ccatgtctcc gcgatggtca ggtcttccag ttgcatgctc
     5401 gaggacgtcg cgacgccatc ggtcaccatt ccggcagacc cctggcttac cgccccgttg
     5461 ctgtcctgga tccaggtact ttcgacgacc acggttctgg actccacgga aacgctgaac
     5521 cgcttgctga tttcagccct ggcgcgatcc gccgcctttt cacgggtatc gccctggcca
     5581 acacccgtca tgaaacgcct ttccggatac ttgtcatgct gtcccagatc gacccaagcc
     5641 ggacgcttgg ccgcggcgga agtggcgcag gatatcgcgc tgactccaat caggacgatg
     5701 ccggcacaga tggccaggct caacggaaaa gtcgtgttga agcggttcat ggtggctatc
     5761 cccttcggtt aagggttgcg gaattacatc tgggtttcgg ccggcgcttc accctgggcg
     5821 gcggccggcg gcggaacttc gacttccatg ccggggccga acttgcgggt catggcgacg
     5881 ccgggacgat ccatgctgta cagtaccaca aaggtcttgg aattcggttc aacattcgct
     5941 tcgaccggaa tcgtgctgac ggtcatgccg ccggcattgg tgaaatcgac attgaacttg
     6001 tggatgccgg gctcgacggg aatgcgggcc atcgtgtaac gattcggcaa cgacatccaa
     6061 ctcctggcgt cggccttttc cacggcctcg gcaatctgga tgctggcact gacgatgccg
     6121 ccaatcaggg cggcccagcc gccgccgctt ccgccgtagt tatttccggc gttcatcgcg
     6181 gcacgggcgg ccttcttggc gacgtacttg gccatggcgc gggcgattgc cttgtccttc
     6241 aactgatcca ggcgctcctt gttgacttcg acagcaacct ttcccatgtc atagacggtc
     6301 gatgtcacat agtccgtgcc gcccatgacg accttcgcgc cttcaacggc ggtcggggac
     6361 gggaagaatt ccggatacgt cacgacgatg ttgtcctgcc cgtcgctctg aatccactta
     6421 aggctttcct tgtgcggaat ctggccaacg ccggtaacaa cgatgatctc ggtcgcggaa
     6481 ggaccttcct tgcaggtgcc gctggcgggc atatcctgcg gattacagac ttccgccttg
     6541 atcctctcga catcagccgg aagaccggcg ggaatctcga cgccgtacct gccgccgacg
     6601 ccctggtagt cctgcagtgc cttcgtatag gccaggcgcg cctgatccag caggttgaac
     6661 cgctgttcgc caccttcctt cgcggccgcg gcctcgaatg caaggccggt catgaaatag
     6721 gcaaagggat cactgacaaa cccggtcgtg acgttgttcc tgcgaagcag ttcaacgaag
     6781 tcggccagct tcgtattcgc ctgctgggcc tcacaccttg cggcttcgta atcgcccatc
     6841 gacaggaagt tcatcgcgtg caggagatcg accatgttcc gttcga
//
LOCUS       DAOPPA010000120         6876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12172_length_6876_cov_4.280457, whole genome shotgun sequence.
ACCESSION   DAOPPA010000120 DAOPPA010000000
VERSION     DAOPPA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12172_length_6876_cov_4.280457"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKH54_05120"
     CDS             complement(<1..677)
                     /locus_tag="PKH54_05120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOC99305.1"
                     /translation="MKARIPAVLAIAVLLLPLVARAFSVQTAYMDDSHKNMSRWAMWD
                     LTYYVDSRGVTGVSATASQNAMTSSFQDWEDIDCSALTFQMLGNSSSQDILPITGTVN
                     GKNELVWVTGSTECRKWNFGSSTLGVTLPMQDYFTGEIYESDIAFNNCDYNGKWAVNK
                     NDLGWGEVSFKSVAIHEIGHFFGVQHSCLDWSEYNENDPATMAPYVDYYGLGDTLNAD
                     DELAARFL"
     gene            complement(674..1309)
                     /locus_tag="PKH54_05125"
     CDS             complement(674..1309)
                     /locus_tag="PKH54_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99306.1"
                     /translation="MSILSVTRSLIRHLLIVPASALFVVVVAFCIPANATTVIHRSIP
                     EMTGVSELVISGVVTQVRQNQPSASGLPSTKVTIAVDKVFKGFASAPTITISIPGGVR
                     DGRIMRIPGMPSFDVGQEVVVFLERAAYGWIPSGLSNGKFQVVTDDRGRRLAWRDTAS
                     LHRIPVNASVTRHATAESDVLLFDDLVEMIQQGLRTTKVPSAASTPEGGAR"
     gene            1426..2259
                     /locus_tag="PKH54_05130"
     CDS             1426..2259
                     /locus_tag="PKH54_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOC99307.1"
                     /translation="MTGIEIIGKDRCKRLEAAVARAGCALKARAASIATVAGTSPHDE
                     NPHTNLDREIDDLLLQVLPEAWGQDPAPAWLSEEKEPPPDRQDSEFVFIIDPIDGTRN
                     VLKGRTDACVSAAMWMRGQGVIWGSVFNPFTNEMFSACKGGGARLNGNLLQVSDVHDV
                     RHAMFLVSVHESVRGMLDRVKQDFKVRPVGSIAYKMCLVACGRGDATFTVNPRNDWDV
                     AAASLIVTEAGGRVTDSRGRSIDINGDSLSIDGLAVTNSTIHDGVLAICDMARRALGL
                     H"
     gene            complement(2383..2667)
                     /locus_tag="PKH54_05135"
     CDS             complement(2383..2667)
                     /locus_tag="PKH54_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOC99308.1"
                     /translation="MKTTLQIVAAVSALTLVGGMLFADELNGDAAEYRRLTSEYAQVQ
                     DKTREARVARDRLRAECRALESSDREFLEKTLRQDFGYVRPNEAVIRFKP"
     gene            3003..3075
                     /locus_tag="PKH54_05140"
     tRNA            3003..3075
                     /locus_tag="PKH54_05140"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3036..3038,aa:Asn,seq:gtt)
     gene            3268..5655
                     /locus_tag="PKH54_05145"
     CDS             3268..5655
                     /locus_tag="PKH54_05145"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOC99309.1"
                     /translation="MFHINGFLNRAPTTRNRFRLSLSATALVLLAMLAASGCGNSGVD
                     VTPGQVMSMDWSKSAGLTAASGCEQAETWIEDAVITQMTLGVEANRRCVLTPDDCGWY
                     RGGVGAEEDWANEGGNPPQANNPDDETPDEYSQTNTQVEGVDEADRVKTDGEYIYVLS
                     DRDLVVVKSWPAAQTGEVGRLTLGSWAYNFFLDGDRAIVLGSANLHEVVKDVPYRTNE
                     GGDDERPGTGGGDQVDGGAPAAEAEGIAIDDVSPDYYYDYRPVTTVTIVSLTDKANPT
                     ILKQFFFEGYTADSRRITDKIYLVLNNYHSVYDLEYWPSDLVMYGSEGEDIRPSDEEI
                     NALFDAMIARNIEKIRARTLAEWMPEYWTSTDGGQPAYADGQMIGECSDVHAPTVFSG
                     QGLLNLVSLDTDSGDIVGSTIQGSWGTVYASLEAVYVGSTNWDFYWWWMGGEDIPPVK
                     THIHKFAFDNDGNARYQASGEVLGYAINQFAYDEYDGYLRVATTDGFGWWNNEETESR
                     VTVLRQDDEKLEQVGVVTGLGLGEQIYAVRFIGDQGYVVTFRQVDPLYVLDLVNPADP
                     KVAGELKIPGFSSYIHPLEPGFLLTAGRDANEEGQVGGIKVEIFDVTDPTAPKSVKTA
                     VIGDGWNTWSDVLWDHKAFTYFGARNLLAIPVSGWVETQGEGGWWGEYKSELALFRVT
                     REDIEILPSISHMDFFDDFGGNDHCYGYGGYWQAQIWRGLFVEDYVYSLSQLGMKVHD
                     TRDLLAGQVAEVTLLDPEYFPGYDWGWCGERVPVDEEEPEEIPPEEGEGDTPRPE"
     gene            5779..>6876
                     /locus_tag="PKH54_05150"
     CDS             5779..>6876
                     /locus_tag="PKH54_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOC99310.1"
                     /translation="MHTILQKHWHHISTENVLEALGVTQDRGLDLFEIGHRRERFGPN
                     KLTERRGQGPLIRFLMQFNQPLVYILLAASTVTLFLSEYVDSAVIFAVVLVNSFVGFV
                     QESKALKAISALAQSLMSEATVLRSGRRERIPSSELVPGDIVFLQSGDKVPADLRLIT
                     VRDLQINESALTGESVPSAKAEGVLAADLPLGDRLNMAYSSTLVTYGTGSGIVVATGD
                     LTEIGRISELIASTNVLATPLTKKIHAFSNMLLWVILGLAGLAFLAGMLHGQNAVDMF
                     MASVALAVSAIPEGLPAAVTIIMAAGVARMAARRAIIRKLPAVETLGSTTVICSDKTG
                     TLTQNQMTVQFINAGGRQFSVTGTGWEPAGQI"
BASE COUNT         1503 a         1997 c         1983 g         1393 t
ORIGIN      
        1 aggaagcggg cggccagttc gtcgtcggcg ttcagcgtgt cgccaaggcc gtaataatcg
       61 acgtaagggg ccatcgtggc cggatcgttt tcgttgtatt ccgaccagtc gagacacgaa
      121 tgctggactc cgaagaaatg accaatttcg tggatggcga cggacttgaa actgacctcg
      181 ccccagccga gatcattctt gttgaccgcc cacttgccgt tgtagtcaca gttgttgaaa
      241 gcgatgtccg attcgtaaat ttcaccagtg aagtagtcct gcatgggcaa cgtgacgcca
      301 agtgtcgaag acccgaaatt ccacttcctg cattccgtgc tgcccgtaac ccatacaagt
      361 tcattctttc cgtttacggt gccggtaatc ggcaggatgt cctgactgct cgaattgccg
      421 agcatctgga aagtcagggc actgcaatcg atatcctccc agtcctggaa ggaagatgtc
      481 atcgcattct gggacgcggt ggccgaaaca ccggtaacgc cacggctgtc aacgtaataa
      541 gtcagatccc acatcgccca tcgggacata ttcttgtggc tgtcgtccat ataggccgtc
      601 tggacggaaa acgcacgggc cacgagtgga agcaacaaaa cggcaatagc gagaacggct
      661 ggaatgcgcg ctttcatctg gcaccccctt ccggagtgga agccgcgctc ggcactttgg
      721 tcgtccggag tccctgctga atcatttcaa ccaggtcatc gaagagaagg acgtccgact
      781 cggcggtcgc gtggcgggta acagaagcgt tgacgggaat ccggtgaagg gacgctgtgt
      841 cccgccatgc aagacggcgc ccccgatcat cggtcacaac ctggaacttt ccgttcgaaa
      901 gacccgaggg aatccatccg taagcggccc gctccaggaa cacgacaact tcctgcccga
      961 cgtcgaacga cggcatccct ggaatgcgca tgatgcgtcc gtcgcgcacc ccaccgggaa
     1021 tcgaaatggt gatcgtcggc gccgaggcga atcccttgaa caccttatcg acggcaatcg
     1081 tgaccttcgt ggaaggcaac cctgacgcgg acggctgatt ctgccttacc tgggttacga
     1141 ctcccgaaat gaccagttcg ctgacccccg tcatttccgg gatggatctg tgaatgacgg
     1201 ttgtggcgtt cgccgggata cagaacgcaa cgaccaccac aaaaagggct gaagcgggca
     1261 cgatcaacaa atgcctgata agcgaacgag ttacggaaag gatgctcaag ggaagcctcc
     1321 agagggcgca gcggccagca gaaaaaaccc gtccaataat aatgaaaggc agttcaaatt
     1381 caactgaatt gctgtaagaa ttcatcgttc gcgggggttt ggttgatgac aggcatcgaa
     1441 atcatcggca aggacagatg caagcgactg gaagccgccg ttgcaagggc cggctgtgcc
     1501 ctgaaggcac gggcggcttc aattgccacg gtcgccggca caagtccgca cgacgagaat
     1561 ccccacacga atcttgaccg tgaaatcgac gaccttcttc tgcaggttct gcctgaagca
     1621 tggggccagg atccggcgcc ggcgtggctt tccgaggaga aggagccgcc acccgaccga
     1681 caggactcgg agttcgtttt cattatcgat cccatcgacg gaaccaggaa cgtcctgaag
     1741 ggacggaccg atgcctgcgt cagcgcggca atgtggatgc gcggccaggg cgtgatctgg
     1801 ggatccgtct tcaatccgtt caccaacgag atgttttcgg catgtaaagg cggcggcgcc
     1861 aggcttaacg gcaacctgct ccaggtatcc gatgtccacg acgtgcgcca cgcaatgttc
     1921 ctggtttccg ttcacgaaag tgtcaggggc atgctggatc gggttaaaca ggatttcaag
     1981 gtgcgtccgg tcgggtcgat tgcctacaaa atgtgcctgg tggcatgcgg acgcggcgac
     2041 gcgactttca cggtcaaccc aaggaacgac tgggacgtgg ccgccgcatc attaattgtt
     2101 acagaggcgg gcggacgcgt tacggactcc cgggggcgat caatcgacat caacggcgac
     2161 agcctgtcca tcgacggact tgccgtgacc aacagcacca tccatgacgg cgtcctggcc
     2221 atctgcgaca tggctcgcag ggcactgggc cttcactgaa aaaatgcggg cgggcgaaat
     2281 ttcattcgca cctacgcctt gtatgggcgg attacatccg cccgctgcga ttcagtattg
     2341 tgccagacgc catcgctgaa gcttcggctt gaatatctga ttctacggct tgaaccggat
     2401 gacggcctcg ttcggcctga cgtatccgaa atcctgtcga agtgtctttt ccaggaactc
     2461 cctgtcggat gattccagcg cgcggcactc ggcccgcagc ctgtcccttg caacccttgc
     2521 ttcacgcgtc ttgtcctgga cctgggcgta ctcgctggtc aatcgacggt actctgcggc
     2581 gtccccgttc agctcgtcgg cgaacaacat tccgccgaca agagttaacg ccgaaacggc
     2641 cgcgacaatc tgtagtgtcg tcttcaacca catgatgcac ggattctaga acagcggatc
     2701 acgcccatac aaaaagacga cggcattttt tttaacaaat ttcgaacact taacacaccc
     2761 ggacagtcaa aatcgacaga ttggccattt tcaccccaat ctgtcgattt tcgacgatac
     2821 aaaatgctgg atccccgcca tggcccggcc ccattaccga tcccgcaatt cgatcggcga
     2881 acagtttcat gcttccgacg gattcgcgat ggacacccga gggctgtcgc ttttctgctt
     2941 gacacccact acccttccgg gcataatttt cgccggttct tttgataacc ttcgttaccg
     3001 atttcggcat agctcagtcg gtagagcaag tggctgttaa ccactgggtc gggggttcga
     3061 gtccctctgc cgaagcaaat tctccgtgcc aagggcccac tccggtgggc ttttttttat
     3121 ccgatcgacg gtacgctcag ggctcttgca accactcgaa atcaatttcg aaaccaatac
     3181 gggcaactcg gttgccgacc ctttgactac gttaactgca atacatatta ttgtttcgta
     3241 ctctttgacc acgaacgagg tagcaaaatg tttcacataa atggattcct gaatcgagca
     3301 ccgacaaccc gaaaccggtt ccgcctttcc ctgtctgcca cggccttggt tcttcttgca
     3361 atgctcgcgg cgtccggttg cggcaacagt ggcgttgacg tcactcccgg ccaggtcatg
     3421 agcatggact ggagcaagtc ggccggattg accgcggcat cgggatgcga acaggcggaa
     3481 acatggatcg aggacgccgt catcacccag atgacacttg gcgtcgaagc gaaccgccgt
     3541 tgtgttctga ctcctgacga ctgcggatgg taccgtggcg gcgtcggcgc ggaggaggat
     3601 tgggcaaacg aaggcggcaa cccgccccag gccaacaatc ccgacgatga gacacccgac
     3661 gagtacagcc agacgaacac ccaggttgag ggtgtggatg aagccgaccg cgtgaagacc
     3721 gacggcgagt acatctacgt gttgagcgat cgcgacctgg tggtcgtaaa gtcctggccg
     3781 gcagcccaga ccggcgaagt tggccgtctg acgcttggtt catgggcgta caacttcttc
     3841 ctggacggcg atcgcgccat cgttcttgga agcgccaacc ttcatgaagt cgtcaaggac
     3901 gttccttacc gtaccaacga aggtggcgac gacgaaaggc ccggcaccgg cggcggtgat
     3961 caggttgatg gcggggcgcc agctgccgaa gccgaaggca tcgccatcga tgatgtatcg
     4021 ccggactatt actacgatta ccgcccggtg accaccgtca caatcgtcag cctgacggac
     4081 aaggcaaacc cgacaatcct caagcagttc ttcttcgagg gatatacggc cgacagccga
     4141 cgcatcaccg ataaaattta ccttgtcctt aacaactacc attcggtcta tgacctggaa
     4201 tactggcctt ccgatctggt catgtacggt tctgagggcg aggacatccg cccctcggac
     4261 gaggaaatca acgccctgtt cgatgcgatg attgcccgca atatcgaaaa gattcgcgcg
     4321 cgcaccctgg ccgaatggat gccggaatac tggacatcca ccgatggcgg gcagccggcc
     4381 tatgccgatg gccagatgat cggcgaatgc tcggacgtgc acgcgccgac tgtcttttcc
     4441 ggccagggac tgctgaacct ggtatcgctt gacaccgatt cgggcgacat cgtcggttcc
     4501 accatccaag gctcctgggg aaccgtctat gccagcctcg aggctgtcta tgtcggatcg
     4561 accaactggg acttctactg gtggtggatg ggtggcgagg acattccccc ggtcaagacc
     4621 cacatccaca agttcgcctt cgataatgac ggcaacgctc gctaccaggc ttccggcgag
     4681 gtcctgggat atgcgatcaa ccagttcgcc tatgacgagt atgacggtta tctgcgcgtg
     4741 gccacgaccg atggattcgg ctggtggaac aacgaagaaa ccgaaagccg tgtcacggtg
     4801 ctgcgacagg atgatgaaaa actggaacag gtcggcgtgg tgacgggact cggactgggc
     4861 gagcagattt acgcagtccg cttcatcggg gatcagggtt acgtcgtgac gttcaggcag
     4921 gtcgacccgc tgtacgtcct cgaccttgtg aaccccgccg atccgaaggt cgccggcgaa
     4981 ctgaagattc cgggattctc cagctacatc cacccgcttg agcctggatt cctgctgacg
     5041 gcggggcgtg acgccaacga agagggacag gttggcggaa tcaaggtcga gattttcgat
     5101 gttaccgatc cgaccgcgcc gaaaagcgtc aagaccgcgg ttatcggcga cggctggaac
     5161 acctggagcg acgtgctgtg ggatcacaaa gccttcacgt acttcggcgc ccgcaacctc
     5221 ctggccatcc ccgtcagcgg ctgggtggaa acgcagggcg agggcggctg gtggggcgaa
     5281 tacaagtccg aacttgccct gttccgcgtg acccgtgagg acatcgaaat cctgccttcc
     5341 atcagccata tggacttctt tgacgacttc ggcggaaacg atcactgcta cggctatggc
     5401 ggatactggc aggcccagat ctggcgcggc ctgtttgtgg aagactatgt ttacagcctc
     5461 agccagctcg gcatgaaggt tcatgacacc cgcgatctgc tggccggcca ggtggcggaa
     5521 gtgacgcttc tggatcccga atacttcccg ggttacgact ggggctggtg tggcgagagg
     5581 gtgccggttg acgaagagga acccgaagaa atcccgccgg aagaaggcga aggcgacacc
     5641 ccccgccccg aatgaccgcg cttgattaat caagtcttcc acattccaac tgaatccagt
     5701 ggcccctgaa ttcaggatta gtattcaaag actcacgatt gccctatcat ttccagacaa
     5761 cacgggagga ccgaagacat gcacacgatc cttcagaaac actggcacca catttcaacg
     5821 gaaaatgtcc ttgaggctct tggtgtaacc caggacaggg gactggatct gttcgagatt
     5881 ggccacaggc gcgagcggtt cggccccaac aagttgaccg agcgacgtgg ccagggcccg
     5941 ttgattcgct tcctgatgca attcaaccag cccctggtct atatcctgct tgcggcttcc
     6001 accgtgaccc tgttcctttc cgagtatgtg gactcggcag taatttttgc agtcgtgctt
     6061 gtcaattcat tcgtgggctt cgtccaggaa tccaaggcgc tcaaggccat cagcgccctt
     6121 gcgcaaagcc tgatgagtga ggcgaccgtt cttcgatccg gcaggcgcga aaggattcct
     6181 tcttcggaac tcgtacccgg ggacatcgtg ttccttcagt ccggggacaa ggtgcccgca
     6241 gacctgcgcc tgataacggt cagggatctt cagataaatg aatcggcgct gaccggggag
     6301 tccgttccat cggcaaaggc cgaaggggtg ctcgccgccg atcttccact cggagaccgg
     6361 ctgaacatgg cctactcctc gacgctggtg acctatggca ccgggtccgg cattgtcgtt
     6421 gccactggcg acctgaccga aatcggtcgg atttcggaac tgatcgcctc gacgaacgtc
     6481 ctcgcgactc ccctgacgaa aaagatacat gcattctcga acatgctgct gtgggtcatc
     6541 ctcggacttg ccggtcttgc cttcctggcc ggcatgcttc atggccagaa cgcggtggac
     6601 atgttcatgg cgtcagtggc gctggcggtg agtgcgatac cggagggact gccggcggcg
     6661 gtgacgatca tcatggctgc cggggtggcg cgaatggccg ccagacgggc gatcatccgg
     6721 aagcttcccg cggtggaaac gcttggaagc accactgtta tctgttcaga caagaccgga
     6781 acgctgaccc agaaccagat gaccgttcag ttcatcaacg ccgggggccg gcagttctcc
     6841 gtcaccggta cgggatggga accggccgga cagatt
//
LOCUS       DAOPPA010000121         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12200_length_6862_cov_8.653151, whole genome shotgun sequence.
ACCESSION   DAOPPA010000121 DAOPPA010000000
VERSION     DAOPPA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12200_length_6862_cov_8.653151"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2912
                     /locus_tag="PKH54_05155"
     CDS             <1..2912
                     /locus_tag="PKH54_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOC99311.1"
                     /translation="VEQQVTIPIEITMGGLPGVRLVRSMSKTALSQVVVVFEDGMDTF
                     LARQLVSERLTMAAADLPEGVVPELGPISTGLGEVFQYTLKGDAHSLTELRTLQDKII
                     TPRLRAIPGVNEVNSFGGLVRQIDVVTDPDRLTKFGLTTGDVVDAIGQDNANAGGSFL
                     INGWEQENIRNVGQFKIPDEIGDVVLSAVDGSPVKIADVSEVREGFMTRLGGVSRDGR
                     GEVVAGMVIMLKDENAAEVVSRVRAALPSIQAALPDDVEIDVFYDRTELVDAVLRTTA
                     SSLLQGAALVIAILFLLLGNIRSALITAVSIPMTALLAFIFMGAAGLSANLMSLDGLA
                     IAIGMIVDGPIVICENITRHWKGLAPGKRLDGLVLAIREVARPIVFSVLIIVLVFVPL
                     FTLEGMEGKMFKPLALTMIFAMIAALIAAMTIVPVLMSFVGGGAGDKGDTRLFARFAG
                     LYQAAVEFVVRHSRITITVAILVFAGTLALIPLIGMEFLPRLDEGAIAVNAVRLPNAS
                     LEGSVKTADYMERTLLKFPEVKTVVSKTGRAEISEDPMGPEQTDLMIMLHPEKEWTTG
                     RDIDQLVGEMREALEEIPGTRFAFSQPIALRVNELVSGVKADLAVKIFGYDLEKLKQA
                     ADETAGILRGIPGARDVSVEQVTGFSQLEVIPDRSAMSRHGIKMSDINDLVETAVGGK
                     VATELIDEQLRIGIQVRVPEGRRSSPEAISELTIRTPSGALVKLGSVATIRRIEGPAQ
                     ISRENGMRRVVVEANVAGRDLGGFVSDSRERLSTLSSELPTGYWLEFGGTFENQKRAM
                     TRLGVVIPISIGLIFLMLVSALGSLRPATMVLLNLPFALAGGLVAMAAFGMPFNVPAT
                     IGFIAVFGIAVQNGTVLLTFVRQLRDEGLSPVEAVIRACRLRLRALLMTSATTVLGLL
                     PMLYATGPGAEIQRPLAVVVIGGLISSTILTLFVLPSIYVAFAEKQVRAIPAMTD"
     gene            complement(2985..4016)
                     /gene="cydB"
                     /locus_tag="PKH54_05160"
     CDS             complement(2985..4016)
                     /gene="cydB"
                     /locus_tag="PKH54_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOC99312.1"
                     /translation="MEHGILQIIWFVLYFVLIAGYAILDGFDLGVGALSLFARDSRER
                     GIHMDSIAPVWDGNEVWLLTAGGALFAAFPFVYATVFSGFYMALMLLLVFLIWRAVSF
                     EFRNKMESPRWIRAWDWAFGLGSVVPPILFGVAVGNILRGLPLNENGDFTGTFVGLLN
                     PYALLVGVLTLVMFLTHGALYLSIKTEGDLRNRMQRIAPGLWIGWVLLWVAGTAVTLF
                     VSPFLFEGILRNPAFWILFPIMLAAFVLIPVFLKSGHQMRAFLASSIAIATQVGLAAV
                     GLFPRMVPARNDLALSLTIDNASSSAKTLTTMLIIAAIGVPIVIAYTVFIYRIFKGKV
                     RHGELYGGH"
     gene            complement(4016..5341)
                     /locus_tag="PKH54_05165"
     CDS             complement(4016..5341)
                     /locus_tag="PKH54_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOC99313.1"
                     /translation="MDAIMLARLQFAMTIGFHFLFPPISIGLAWLLVFIEFKGMRTGD
                     HVWIRLGKFFGRLFALTFAVGVATGIVMEFQFGTNWASYSRFVGDIFGAPLAAEGIFA
                     FFLESSFLGLYLFGRNKVGKGVHWFSILMVAVGATFSAFWILIANSWQQTPAGFEIVD
                     GRAVLTSFSQAVFNPSMWHRFFHTLAACLITGAFFLGGISAWRLLRDRTDEVARRGLL
                     VAVMSGFVFSVTAAFPTGDNHARQVARTQPEKFAAIEGIYETRQGAPLVVFGIPRKDP
                     LRLDNAVEIPKLASWLAYGDPNAEVKGLDAFKTDEIPPIFLTFVSFHTMVALGMLFIA
                     VMGLGLLLLWRRRLATSRWYHWILLVVSPLTLAASQLGWITAEVGRQPWSVYRLLRTA
                     DSVSVTVGIPELWFSIGLIGFMYILLGGLYIFMLVREVRRGPDKAPEVV"
     gene            5520..>6862
                     /locus_tag="PKH54_05170"
     CDS             5520..>6862
                     /locus_tag="PKH54_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOC99314.1"
                     /translation="MSHARKNENVRNVAIIAHVDHGKTTLVDAMFRQSGVFREGQQVA
                     DRIMDNMELERERGITIAAKNCSVIWDDVKINIIDTPGHADFGGEVERALSMADGAVL
                     LVDAAEGPLPQTRFVLKKTLEAGLRVIVVINKIDRKDARPEEVLNEVFDLFIDLGADD
                     EQLDFPCLYAVGRDGVAMRGLSDARQDLRCLFETILTEIPAPLFDPDEPFQMLVSDLD
                     YSDYVGRLAIGKVAHGHVTSADALTCIGADGGCKPLKVTRLQNYEGISIKDVDTVEPG
                     DIVVLAGIEDVMIGDTICTQAAPKALPRLTVDEPTVFMRIFCNTSPLAGTEGTIFQPN
                     RIHDRLLKETLRNVSIRLEVPTDGEGFIIKGRGEFQLAILIETMRREGFELCVSRPEV
                     ICRNIDGKMLEPIEHLFVDCGDTYQGIVTQKVSIRKGRMINYGSLGTGRVRIEFT"
BASE COUNT         1529 a         2035 c         2108 g         1190 t
ORIGIN      
        1 acgtggaaca acaggtgacc atcccgatcg agatcacgat gggcggcctg cccggggtca
       61 ggcttgtcag atcgatgtca aagaccgccc tttcgcaggt cgtcgtcgtt ttcgaagacg
      121 gaatggatac atttctggcc cgccaactgg tatccgaaag actgaccatg gctgcggccg
      181 acctgcccga gggtgtcgtg cccgagctgg gaccgatcag tacgggtctg ggcgaggtct
      241 tccagtacac gctgaagggc gacgcccatt ccttgaccga actcagaacg ctgcaggaca
      301 aaatcatcac cccgcgactg cgcgcgatcc ccggggtaaa cgaggtgaac agcttcggcg
      361 ggctggtgcg ccagatcgac gtggtcaccg acccggacag actgaccaag ttcggtctga
      421 ccactgggga tgtcgtggac gccatcggcc aggacaacgc caacgccggc ggatcgttct
      481 tgattaacgg atgggagcag gaaaacatcc gtaatgtcgg gcagttcaag attcctgacg
      541 agatcggcga cgtggttctt tcggcggttg acggatcgcc ggtcaagatc gccgatgtct
      601 ccgaggtgcg cgaaggcttc atgaccaggc tgggcggcgt atcgcgcgac ggtcgcggcg
      661 aggtggtggc cggcatggtt atcatgctga aggacgaaaa cgccgcggag gtcgtgagcc
      721 gcgtcagggc ggccctgcca tcgatccagg ccgccctgcc cgacgacgtg gagatagacg
      781 ttttctacga ccgtaccgaa ctggtcgacg cggtgttaag gacaactgcc agcagtctgc
      841 tgcagggcgc cgcgctggtc atcgccatcc tgttcctgct gcttggaaat atcaggtcgg
      901 cgctgataac ggcggtcagc ataccgatga cggccctgct ggcattcatc ttcatgggcg
      961 ccgctggact gtccgcgaac ctgatgagcc ttgacgggct ggccatagcg atcgggatga
     1021 tcgtggacgg gcccatcgtg atttgcgaaa acatcacgcg ccactggaag ggactggccc
     1081 ctggaaaacg gctggacggt cttgtgctgg caatccgcga ggttgcgcgg ccaatcgtgt
     1141 tttccgtcct gatcatcgtg ctggtattcg tgcctctttt cacattggaa ggcatggaag
     1201 gcaagatgtt caagccgctc gcgctgacca tgatttttgc gatgatcgcg gcgctgatcg
     1261 cggcgatgac gatcgtgccg gtcctgatgt cattcgtggg gggcggtgca ggggacaagg
     1321 gcgacaccag gctgttcgcg cggttcgccg gcctgtacca ggccgcggtc gaattcgtcg
     1381 tcaggcattc caggattacc atcacggtgg cgattctggt gttcgccggc acgctggctc
     1441 tgatcccgtt aatcggcatg gaattcctgc cgcggctcga cgagggcgcc atcgcagtca
     1501 acgcggtccg cctgccgaac gcgtcgcttg aaggctcggt caagacggcg gactacatgg
     1561 aaaggacctt gctgaagttc cccgaggtga aaaccgtcgt ttccaaaacc gggcgggccg
     1621 agatctcgga agacccgatg ggtccggaac agacggatct gatgatcatg ctgcacccgg
     1681 aaaaagaatg gacgaccgga cgcgacatcg accagcttgt aggcgaaatg cgtgaagccc
     1741 ttgaggaaat ccccggcacc cggttcgcgt tcagtcaacc tatcgcactg cgggtgaacg
     1801 aactggtttc aggtgtaaag gccgacctgg cggttaaaat cttcggatac gaccttgaaa
     1861 agctgaagca ggccgcggac gaaaccgcag gcatcctgcg cggcatcccc ggggcgcgcg
     1921 acgtcagcgt cgaacaagtc acaggcttca gtcagctcga ggtcataccc gaccgctcgg
     1981 cgatgtccag gcatggcatt aaaatgagcg atatcaacga tctggtcgag acggctgtcg
     2041 gcggaaaggt cgcaaccgaa ctgatcgacg agcaactgcg aatcggcata caggttcgcg
     2101 ttcccgaagg acgacgcagt tcgcctgagg caatctcgga actgaccatc cgcaccccat
     2161 cgggcgccct ggtgaaactt ggcagcgtgg ccacgatcag gcgcatcgag ggtccagccc
     2221 agatcagtcg cgaaaacggc atgcgacggg tcgtggtcga ggccaacgtg gccggccggg
     2281 atctgggcgg cttcgtcagc gattcccgcg aacggctttc gacgttgtcg agtgaactcc
     2341 caaccggata ctggctggaa ttcggcggaa cctttgaaaa ccagaagcgg gccatgacgc
     2401 gcctgggcgt ggtcatcccg atctcgatcg ggctgatatt cctgatgctg gtgtcggcgc
     2461 tcggctcgtt acgaccagcc acgatggtac tgctgaacct gccgttcgcg ctcgccggcg
     2521 ggctggtggc gatggccgcc ttcggcatgc cgttcaacgt gcccgcgacc atcggtttca
     2581 tcgcggtttt cggcatcgcg gtccagaacg gcacggtgct gctgactttc gtccgtcaac
     2641 ttcgcgacga gggactatcg ccagtcgagg cagtcatccg agcctgccgc ctgcgtctgc
     2701 gagcgctgct gatgacgtcc gccacgaccg tcctgggcct gctgccgatg ctgtacgcga
     2761 ccgggcccgg tgccgagatc cagcgccccc tggccgtggt cgtcatcggc ggcctgattt
     2821 catcgacgat cctgaccctg ttcgtcctgc cgtcgatcta cgtggctttc gcggaaaaac
     2881 aggtccgtgc gatacctgct atgactgatt agcggaatgc tgcatttcct ggcaagggaa
     2941 gcggcctgaa gtagtccgga aaatcggata ttcgtcagat cccttcagtg cccgccgtac
     3001 aactcgccgt gacggacctt gcccttgaat attcggtaga tgaacaccgt gtacgcgatc
     3061 acgatcggca ctccgatagc ggcaatgatc agcatcgtgg tcagtgtctt cgcggacgaa
     3121 gacgcgttgt cgatggtcag gctcagagcc agatcgtttc tggcgggaac catgcgcggg
     3181 aacagcccca cggcggccag tccgacctgg gtggcgatgg cgatcgacga cgccaggaac
     3241 gcgcgcatct gatgaccgga cttcagaaac accggaatca ggacaaacgc cgccagcatt
     3301 atgggaaaca gaatccagaa tgccgggttc cttagaatgc cctcgaacag gaaaggcgaa
     3361 acaaacagcg tcaccgccgt accggcaacc cacagcagga cccagccgat ccacagaccg
     3421 ggggcgatgc gctgcatccg gttgcgcagg tcaccctcgg tcttgatgga gaggtacaac
     3481 gccccgtggg tcaggaacat gaccagcgtc aggacgccga cgagcagcgc gtacggattc
     3541 agcagcccga cgaatgtgcc ggtgaagtcg ccattttcgt tcaggggcag tccccgcagg
     3601 atgttgccga ccgccactcc aaacaggatc ggtggaacga cgcttcccag gccgaacgcc
     3661 cagtcccagg cacggatcca gcgcggcgat tccatcttgt tgcgaaactc gaacgacacc
     3721 gcacgccaga tcaggaagac aagcagcaac atcagcgcca tatagaaacc ggaaaacacc
     3781 gtcgcgtaca caaacggaaa cgcggcgaac agagcgccgc cggcggtcag cagccagacc
     3841 tcgttgccat cccagaccgg ggcgatcgaa tccatgtgga tgccacgttc gcgggaatca
     3901 cgcgcgaaca gcgacaaagc tccgacacca aggtcaaaac cgtccaggat ggcgtatccg
     3961 gcgatcaata cgaaatacag cacgaaccag atgatctgaa ggattccgtg ttccatcaca
     4021 ccacctccgg ggccttgtcc ggcccgcgcc tgacctcgcg cacaagcatg aagatgtaca
     4081 ggccacccag caggatgtac atgaacccga tcaggccgat gctgaaccag agttcaggta
     4141 tgccgaccgt caccgacacc gagtcggctg tgcgaagaag gcgatagact gaccacggct
     4201 gacggccgac ctcggcggtg atccagccga gctgcgacgc ggccagtgtg agcgggctca
     4261 cgacaagcag gatccagtga taccagcgtg acgtggcaag gcgcctgcgc cacaaaagca
     4321 gaaggccaag ccccatcacc gcgatgaaaa gcatcccgag agccaccatc gtatgaaatg
     4381 acacgaaggt cagaaagatt ggtgggattt cgtcggtctt gaacgcatca aggcccttca
     4441 cctcggcgtt tggatcgccg tatgcgagcc agcttgccag tttcgggatc tcgacggcgt
     4501 tgtcgaggcg caacggatcc ttgcgcggaa tgccgaacac caccagaggc gcgccttgcc
     4561 gcgtttcata gataccctcg atggcggcga atttctcggg ttgagtccgg gcgacctgac
     4621 gtgcgtgatt atcaccggtc gggaaagccg cggtcaccga gaagacgaat ccagacatga
     4681 ccgcgaccag cagaccgcga cgcgcgactt cgtcagttcg gtcgcgcagc agacgccagg
     4741 cactgatgcc gcccaggaag aaggcaccgg taatcaggca ggccgccaga gtgtggaaga
     4801 agcggtgcca catcgacgga ttgaagacgg cctgcgagaa actggtgagc acggcacggc
     4861 cgtccacgat ttcgaagccg gccggggtct gctgccaact gttcgcgata aggatccaga
     4921 acgcggaaaa ggtcgctccg accgcgacca tcaggatcga gaaccagtga acgcccttgc
     4981 caaccttgtt gcggccgaac agatacaatc caaggaagct ggattccagg aagaacgcga
     5041 agatgccctc ggccgccagg ggcgcgccaa agatgtcgcc gacaaaacgc gaatacgagg
     5101 cccagttcgt gccgaactga aactccatca cgatgccggt cgccacaccc accgcgaatg
     5161 tgagggcgaa aagccgaccg aagaatttgc ccagcctgat ccagacgtgg tcaccagtcc
     5221 gcatcccctt gaactcgatg aaaaccagca gccatgcaag accgatagat atcggcggga
     5281 acaggaaatg aaacccgatc gtcatcgcga actgcagtcg ggccagcatt atcgcgtcca
     5341 tcaaagacct ccgcaggtat ggccccggtg attccggtat gaaagaagct tggctgaagg
     5401 acgggtgcaa gtcaaccaga ccgacgagca tggcaattcg ggcaaaaaag cctatactcc
     5461 gcgtccgttg ccgtcagcag ataccgcgga cgctgcagaa tcaccatgaa aggaatgcat
     5521 tgagtcacgc cagaaaaaac gagaacgtca ggaacgtcgc gatcatcgcc cacgtcgatc
     5581 acggcaagac caccctggtt gacgccatgt tccgtcaaag cggcgtgttc cgggaaggtc
     5641 agcaggtcgc ggacagaatc atggacaaca tggagctgga acgcgagcgc ggtatcacaa
     5701 tagcggccaa gaactgctcg gtcatctggg acgacgttaa gatcaatatc atcgacaccc
     5761 ccggacacgc ggacttcggc ggcgaggtcg aacgtgcatt gtcgatggcc gacggcgccg
     5821 tcctgctggt cgacgcggcg gaaggccccc tgccccagac cagattcgtc ctgaagaaga
     5881 cgctggaagc cggattgagg gtcattgtcg tcatcaacaa aatcgacagg aaggacgcca
     5941 ggccggaaga agtcctaaac gaagtattcg acctgttcat agacctcggc gccgacgacg
     6001 aacagctgga ttttccctgc ctttacgcgg ttggacgcga cggcgtggcg atgcgcgggc
     6061 tgagcgacgc caggcaggat ctgcgctgcc tgttcgagac catcctgacc gagattccgg
     6121 ccccgttgtt cgatccggat gaacctttcc agatgctggt atccgatctg gattactcgg
     6181 attatgttgg caggctggcc atcgggaagg tggcacacgg ccatgtcaca tcggcggacg
     6241 cgctgacctg cattggcgcc gatggcggat gcaaaccgct gaaagtcacc cggctgcaga
     6301 actacgaagg catcagcatc aaggacgtcg acaccgtcga gcccggcgat atcgttgtac
     6361 tggccggcat cgaagacgtg atgattggcg acacgatatg tacccaggcc gctcccaagg
     6421 cgctgccgcg gctcactgtc gacgaaccga ctgttttcat gcggatattc tgtaacacat
     6481 cgccgcttgc cggcaccgag gggacgatct tccagccgaa caggattcac gaccggctgc
     6541 tgaaggagac cctgcgcaac gtgtcgatcc gacttgaggt cccgactgac ggcgaaggtt
     6601 tcatcatcaa gggccgcggc gagttccagc ttgccatcct gatcgaaaca atgcgtcggg
     6661 aaggcttcga gctgtgcgtc agccggccgg aagtgatctg caggaacatc gacgggaaaa
     6721 tgctggagcc gatcgagcat ctcttcgtcg actgcggaga cacttatcaa ggcatcgtca
     6781 cccagaaggt aagcattcga aaaggcagga tgatcaatta cggaagcctc ggaaccggcc
     6841 gagtcagaat cgaattcacg at
//
LOCUS       DAOPPA010000122         6832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12269_length_6832_cov_3.745905, whole genome shotgun sequence.
ACCESSION   DAOPPA010000122 DAOPPA010000000
VERSION     DAOPPA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12269_length_6832_cov_3.745905"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="hemW"
                     /locus_tag="PKH54_05175"
     CDS             complement(<1..952)
                     /gene="hemW"
                     /locus_tag="PKH54_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOC99315.1"
                     /translation="MSTCFGLYVHIPFCRTKCPYCAFDSAPDQDDLIDEYIETLCDEI
                     AARPEQARSFDTVYFGGGTPGLLSEEHMGRITDFIRSELFVKPDAEWTIEVNPGDVTI
                     DKASFWVKKAGFNRISLGVQSFEQNELDFLGRRHDVQDALDAISDCQMTGAKICIDLI
                     QGLPGQNTEQRMRSVHKAASLKPSHISVYELTIDEGSAFHAAGAKFHRPDQDAAADGY
                     LATSKYLRGKGYLHYEVSNYCLDTFDNMAAHNARYWQGRPWLGVGASAHSFDGTRRWW
                     NVRGAAAYIQAMNQAENAIEDTEALTDDQLRLERVALGFRW"
     gene            complement(1008..2804)
                     /gene="lepA"
                     /locus_tag="PKH54_05180"
     CDS             complement(1008..2804)
                     /gene="lepA"
                     /locus_tag="PKH54_05180"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOC99316.1"
                     /translation="MVNVRNFSIIAHIDHGKSTLADRLIQHCGLVSDREFHDQLLDNM
                     DIERERGITIKSQTARLPFVAADGRQYFLNLIDTPGHVDFSYEVSRALKSCEGVVLLV
                     DASQGVQAQTVANMYLAMENGLEIIPVINKIDLPSADIDMCLEQIDRELGLFPEETVM
                     VSAKTGKGVPELLQAVVERIPEHKGNPGAPLQALIFDAFYDPFRGAVASIRVMNGTVK
                     PGQIARMMSTGATHRIEEVGIYSINRTAVKQLVAGDVGYIIAGVKTVADTRIGDTVTT
                     EDNPATSPLEEFRASKPVVFSSLYPVSTEDYQELADALEKYKLNDAALTYEKDSSTAL
                     GQGFRAGFLGLLHLEIVQERLEREFDQSLILTAPSVEYHFFLEDGSMAVVDNPAHYPD
                     PVYIRSAEEPYIRASIMIPDRYMGAVMKLCLEKRGENSELGYPAPGRLEMRFDMPLAE
                     VIFDFYDKLKSITQGYGSFDYEMLDYRKSDLVKLDILVNGEKVDALSMIVHRDNARAR
                     ALQACERLSEEIPRQQFKVPIQGAIGGKIIARETIQPFRKDVTAKCYGGDISRKRKLL
                     EKQKEGKRKMKIVGDVEIPQKAFIAVLKTKDD"
     gene            complement(2879..3457)
                     /locus_tag="PKH54_05185"
     CDS             complement(2879..3457)
                     /locus_tag="PKH54_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99317.1"
                     /translation="MTRKAALLFVAVTLMATGAVAAEGNTHIGFQGQFIAYGVQREAG
                     VNTDMQVPSGGLVFFAEYGVLDYIALGGQIEYGNIYTPGSNPEDPFNSAMRNFMGFSG
                     SMRVGYPFLDKKNLDVYVRLNVGYGLYGLSQQDLMHGWVARAMPGVMYTTKSGFAVFA
                     EIGWAGGGFINESDNHVFYNGLAIDFGIGWKF"
     gene            3706..4593
                     /locus_tag="PKH54_05190"
     CDS             3706..4593
                     /locus_tag="PKH54_05190"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOC99318.1"
                     /translation="MSTTVTSTNFTEFPLISKGKVRDIYDLGENLLIVVTDRLSAFDH
                     ILPQPIPDKGRVLNLMSKFWFEMTADKLKNHMISTEVADLPQSLHKYGDILQGRFTLA
                     HKAKMVPVECIVRGYLSGSGWNDYKRTGAVCGIKLPEGLQDSSKLPSPLFTPSTKAES
                     GHDENISFDQAVEIVGAEVAARIRDASLMLYTTAADYALTRGIIIADTKFEFGFIDGE
                     LIVCDEVLTPDSSRFWPADQYAPGRAQPSFDKQYVRDYLLQIGWNKEPPIPSLPPEVV
                     ANTSSRYVDAYMLLTGRSL"
     gene            complement(4713..6056)
                     /locus_tag="PKH54_05195"
     CDS             complement(4713..6056)
                     /locus_tag="PKH54_05195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOC99319.1"
                     /translation="MSLRLKILLLTIGIVVFAVAGTARLIAKGMYERLVTESELRAVA
                     LIEGVALSVRDDLACERLDEIGKSLIQLRDPGSGRSDVIFTLILDGNGRAVGREVMPE
                     YEQLLKSDFVRQATAASRELKTHVTFDGRDIVLVSIPVISSVPHIEGVRWGTVVGGMD
                     IGRIEAAMFPLVRYAVGAALGFLLIGVFLLVLTLYRNLLSPINKLAEAASRFAAGDLQ
                     FRAPQGTADELGRLGATMNQMASDIEQHTRTLEDAVKARTRELEQANSRLHELAAMDE
                     LTGLGNRRTFTAALNREFRRARRDGSCISMLMLDVDHFKKYNDTHGHPAGDVALNKVG
                     AILRNHLRVTDIPCRYGGEEFAVILCQTARDDGMRIADDIRRVVQQEVFFGEDRQPLG
                     TFTVSIGVSTFPRDADDPDKLVDAADIALYQAKASGRNQVRGFEPGMTAKANLSN"
     gene            complement(6053..>6832)
                     /locus_tag="PKH54_05200"
     CDS             complement(6053..>6832)
                     /locus_tag="PKH54_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOC99320.1"
                     /translation="TGQPDPLIDLASYIKSRTGLEVEFVRLNAYPLFTSGLIDGSLQG
                     GLFPPGEYLRARKIDPCIHLVATTMYGTSTTYTTNLVVRRDSDIMVPSDLVQKRVAFS
                     SRSSASGYIYAVKFLADRGLIPERDYIPVFTGGHQETIRAVEIGTVAAGATFGRALNV
                     EMQIGRDLTGLSILAVAGQIPNEPLVFASDLDPDIIRAMTDAFLSLNRGTAEGRKLLG
                     ALDLMSGWVRADDSFYDGLMTTVQAVDRIVRSADSGGEIGR"
BASE COUNT         1378 a         1995 c         2033 g         1426 t
ORIGIN      
        1 tccagcggaa gcccagcgcc acgcgttcaa ggcgaagctg gtcgtcggtc aatgcttccg
       61 tgtcttcgat ggcgttttcg gcctggttca tggcctggat ataggccgcg gcgccccgga
      121 cattccacca gcggcgcgtg ccgtcgaagc tgtgggcgga cgcgccgacg ccaagccacg
      181 gacggccctg ccagtagcgc gcgttgtggg cggccatgtt gtcgaacgtg tccaggcagt
      241 aattggagac ttcgtagtgc aggtacccct tgccacgcag gtacttcgac gtcgccaggt
      301 aaccgtcggc cgcggcatcc tggtcggggc ggtggaactt ggcgccggcc gcgtggaagg
      361 cgctgccttc atcaatggtc agttcataca ccgagatgtg tgaaggcttc agggacgctg
      421 ccttgtggac tgaacgcatg cgctgttcgg tgttctggcc gggaagtccc tggatcaggt
      481 cgatgcagat cttcgcgccg gtcatctggc agtcagagat cgcgtcaagc gcgtcctgga
      541 cgtcgtggcg gcggcccaga aaatcgagtt cgttctgctc gaagctctgg acgccaaggc
      601 tgattcggtt gaagccggcc tttttgaccc agaacgacgc cttgtcgatg gtcacatcgc
      661 cgggattgac ctcgatcgtc cattcggcgt cgggcttaac gaaaagctcg gatcggatga
      721 aatcggtgat gcgccccata tgctcttcgg aaagcaggcc gggggtgccg ccgccgaaat
      781 atacggtgtc gaacgatcgc gcctgctcgg gacgtgccgc gatttcgtcg cagagcgtct
      841 cgatgtattc atcaatcagg tcgtcctggt cgggtgccga gtcgaacgcg cagtaagggc
      901 atttggttcg gcagaacggg atgtggacgt aaaggccgaa acaggtggac atacagaaac
      961 ctcagagcgg gcggatgcga tccgcttgaa caaggcacaa ggcacggcta atcgtccttg
     1021 gttttcagga cggcaatgaa agccttctgc ggaatttcga cgtcgcccac gatcttcatc
     1081 ttccgcttgc cttccttctg cttttccagc agcttgcgct tacgactgat gtcgccgccg
     1141 taacacttcg cggtgacgtc cttgcggaac ggctggatcg tctcgcgggc gatgatcttg
     1201 ccgccgatgg caccctggat tggcaccttg aactgctggc gcgggatttc ctccgaaagc
     1261 cgttcgcagg cctgaagggc gcgcgcgcgc gcattgtcgc ggtgaacgat catcgaaagc
     1321 gcatccacct tctcgccgtt gaccaggatg tccagcttga ccaggtcgga cttgcggtaa
     1381 tcaagcattt catagtcgaa gctgccgtaa ccctgggtaa tcgacttcag cttgtcgtag
     1441 aagtcgaaga tgacttcggc aagcggcatg tcgaaccgca tttccaggcg accgggcgcg
     1501 ggatatccta gctcgctgtt ttcgccgcgc ttttcaaggc agagcttcat cacggcaccc
     1561 atgtatcgat ccgggatcat gattgatgcc cggatatatg gttcctctgc ggaacggata
     1621 taaacgggat ctggatagtg ggccgggtta tcgacaacgg ccatcgaccc gtcttccagg
     1681 aagaagtggt actcgacgga cggcgcggtc agaatcagcg actgatcgaa ctcgcgctcc
     1741 agcctttcct ggacgatttc caggtgcagc aggcccagga accccgcccg gaacccctgg
     1801 ccaagcgcgg tcgatgagtc cttttcatag gtcagagccg cgtcgttcag cttgtacttt
     1861 tcaagggcgt cggccaactc ctggtaatct tcggtcgaaa caggataaag cgatgaaaag
     1921 acgaccggct ttgaagccct gaattcttcc agcgggcttg tcgccgggtt gtcttccgtc
     1981 gttaccgtgt cgccgatgcg ggtgtccgcc accgtcttga cgccggcgat gatgtagccg
     2041 acgtccccag cgaccagttg cttgaccgcc gtgcggttga tgctgtagat gccgacttct
     2101 tcgatccggt gggtcgcgcc ggttgacatc atccgggcga tctggcctgg cttgaccgtt
     2161 ccgttcatga cccggatgct ggcaacagcc ccgcggaacg ggtcatagaa cgcatcgaaa
     2221 atcagggcct gcaatggcgc cccgggattc cccttgtgtt cggggatgcg ttccacgacg
     2281 gcctgcaaca gttccggaac gccctttccc gtcttggccg aaaccatgac ggtttcctcc
     2341 gggaaaaggc cgagttcacg gtcgatctgt tccaggcaca tatcgatatc cgcggaagga
     2401 aggtcgattt tattgatgac cggaatgatt tccaggccgt tttccatcgc aagatacatg
     2461 ttggcaacgg tctgggcctg aaccccctgg gatgcgtcca caagaagcac gacgccctcg
     2521 caggatttca gagcccgcga gacctcgtaa gagaaatcaa catgcccggg ggtgtcgatc
     2581 aggttaagaa agtactgccg gccgtcggca gcgacaaagg ggagtcgggc ggtctggctt
     2641 ttgattgtga tgccgcgttc cctctcgatg tccatgttgt ccaggagctg gtcgtgaaac
     2701 tcgcgatcgg agaccagtcc gcagtgctgg ataaggcggt cggccagtgt cgattttccg
     2761 tggtcgatgt gggcgatgat gctgaagttg cggacgttaa ccatagaagg tgaaatcccc
     2821 gttaatacct gtaagccggc ttcgaatcgg tggagggcgg gcgcagcgag tcgagggatc
     2881 agaacttcca gccgataccg aaatcgattg caagcccgtt ataaaagacg tgattgtcag
     2941 attcgttgat gaatccgccg ccggcccatc cgatttccgc gaaaaccgcg aagccggact
     3001 tcgtcgtgta catcacgccg ggcatcgccc tggcaaccca accgtgcata agatcctgct
     3061 ggctcaggcc gtaaagacca tagcccacgt tcagccggac atagacatcc aggttcttct
     3121 tgtccaggaa cggatatccg acccgcatgc ttcccgaaaa gcccatgaag ttgcgcatgg
     3181 ccgaattgaa gggatcctcg ggattcgagc ccggggtgta gatgttgccg tactcgatct
     3241 ggccgccgag cgcgatgtaa tcaagaacgc cgtactcagc gaagaaaacc aggccgcccg
     3301 aaggtacctg catgtcggtg ttgacacccg cttcacgctg gacgccgtag gcaatgaact
     3361 gtccctggaa gccgatgtgg gtgttgcctt cggcggcgac ggcacccgtc gccatgagcg
     3421 tgacagccac gaacagaagg gccgctttcc gtgtcatctg gaatcccctg tttcaaggac
     3481 ggggcagctt acggtgcccc cgggttacac aatccgacag atactaatag gaaagcgggt
     3541 tgcgtggcaa gggaaaaagg tgcaggaaat ccggttttgc aacagctgcc gtcgttcgcg
     3601 tcggtcgcga ccacaatgac gagagtgtcc ccttttttcc gtgccggtgt cactgtagaa
     3661 tcctgtcctg cgtcgccagg gtggcgtcgc aatagtggag gaatcatgag caccaccgtc
     3721 acttcgacca acttcaccga gtttccgctg atctcaaagg gcaaggttcg tgacatctat
     3781 gatcttggcg aaaatctcct gatcgtcgtg accgaccggc tgtcggcatt tgatcacatc
     3841 ctgccgcagc caattcccga caagggccgc gtcctgaacc tgatgtcgaa gttctggttc
     3901 gaaatgacgg ccgataagct gaagaatcac atgatttcaa cggaagtcgc ggatttgccg
     3961 cagtcgcttc acaagtacgg cgacatcctt cagggacgtt tcacgcttgc ccacaaggcg
     4021 aagatggtgc cggttgaatg catcgttcgc ggttacctgt cgggatccgg ctggaacgac
     4081 tacaaacgca cgggcgcggt ctgcggcatc aagctgccgg aagggttgca ggattcgagc
     4141 aagttgccgt cgccgctctt tactccgtca accaaggctg aaagcggtca tgatgaaaac
     4201 atcagcttcg accaggccgt cgagattgtc ggcgcggaag tggccgccag gattcgtgac
     4261 gcatccctga tgctttacac gaccgcggcc gactacgcgc ttacccgggg tatcatcatc
     4321 gccgacacca aatttgaatt cggtttcatc gacggtgaac tgattgtctg cgatgaagtc
     4381 ctgacgccgg acagcagtcg cttctggccg gcggatcagt acgcgccggg ccgcgcgcag
     4441 ccgtccttcg acaagcagta cgtccgcgac tacctgttgc agatcggttg gaacaaggaa
     4501 ccgcccattc ccagcctgcc accggaagtc gtggccaata cttcgtcacg ttatgtggac
     4561 gcttacatgc ttctgaccgg ccgttccctg taggttccgt cgcggatttt ccggttcatg
     4621 ttccggattg ccaggattgt tcgaaccaac cggtttatcc gccgaaccgc atcgtcatcg
     4681 taatgccgac ccgcagacgg gaatcaaacg gttcaattgg acaggttggc cttggcggtc
     4741 atgccgggtt cgaacccgcg aacctggttt cgtcccgatg ccttggcctg gtaaagcgca
     4801 atgtccgccg catcgaccag cttgtccgga tcgtcggcgt cacggggaaa agttgaaaca
     4861 ccgatggaca cagtgaaggt tccaagcggc tggcgatctt ccccgaaaaa gacttcctgc
     4921 tgcacgacgc ggcggatgtc gtccgcaatc cgcatcccgt catcccttgc ggtctggcac
     4981 aggataaccg cgaattcctc gccgccgtat cggcacggta tgtccgtcac gcgcaaatgg
     5041 tttcgaagga tcgcaccgac cttgttcagg gccacgtcgc cggccgggtg gccgtgcgta
     5101 tcgttgtatt tcttgaaatg gtcaacatcg agcatcagca tcgaaatgca ggagccgtcg
     5161 cgtctggccc gcctgaattc acgattcagc gcggcggtga atgtgcgccg gttaccaaga
     5221 ccggtcaatt cgtccatggc cgcaagttcg tgcaatctgg aatttgcctg ctccagttcg
     5281 cgggtccttg ccttgaccgc gtcctcaagt gtccgggtgt gctgttcgat atcgctggcc
     5341 atctggttca tcgtcgcccc gagtcggccc agctcgtcgg ccgtgccctg cggcgctctg
     5401 aactgaaggt cgcctgcggc gaagcgcgac gcggcctcgg ccagcttatt gatcggtgaa
     5461 agcaggtttc tgtaaagcgt cagaaccaga aggaacaccc cgatgagcag gaatccgagt
     5521 gccgcgccaa ccgcgtacct gaccagcgga aacatcgcgg cctcgatgcg gccgatgtcc
     5581 attccgccga caacggttcc ccagcgaacg ccctcgatgt ggggaacgct gctgatgacc
     5641 gggatgctga ccaggacgat gtccctgcca tcgaaggtga catgtgtctt cagttcccgg
     5701 cttgccgcgg ttgcctgtcg aacgaagtca ctcttcagga gttgctcgta ttccggcatc
     5761 acttcgcgtc cgaccgcccg tccattgccg tcaaggataa gagtgaagat gacgtcggat
     5821 ctgccggatc ccggatcgcg aagttgaatc agggatttcc cgatttcatc cagtctctcg
     5881 caagcaaggt cgtcacgaac cgacaaggca acgccctcga tcagcgcgac cgcccgaagt
     5941 tcggactcgg taaccagccg ttcgtacatg ccctttgcaa taaggcgcgc ggttcccgca
     6001 acggcgaaaa caacgattcc gatcgtcagc agcaggattt tcaggcgaag gctcatcgtc
     6061 cgatctcccc gcccgagtca gccgagcgaa cgattctgtc aaccgcctgg acagttgtca
     6121 tcaatccgtc gtagaacgaa tcatccgcgc gcacccatcc ggacatcagg tcaagcgccc
     6181 cgagcagctt tcgtccctct gcggttccgc ggttaaggga caggaaggcg tcggtcatcg
     6241 cccggatgat gtccggatcg agatccgatg cgaaaaccag aggttcgttc ggtatctggc
     6301 cggcgaccgc aaggatcgac agaccggtaa ggtcgcgtcc aatctgcatt tcgacgttca
     6361 aggcccgtcc gaaagttgcc cctgcggcga cagtgccgat ttcaaccgcc cgaattgttt
     6421 cctgatggcc gccagtaaag accgggatgt agtcgcgctc gggtatcagt ccccgatcgg
     6481 caaggaactt gaccgcatag atataaccgg aagccgagct tcgggaggaa aaagcgaccc
     6541 gcttctggac aagatccgac ggcaccatga tgtccgaatc gcgccgaacg accaggttgg
     6601 tcgtgtaagt cgttgacgtt ccgtacatcg tggtcgcgac aaggtggata caggggtcga
     6661 ttttgcgcgc cctcagatac tcccctggcg ggaacaggcc gccctgcagg gaaccgtcaa
     6721 tcaggccgct tgtgaaaagg gggtacgcgt tcagccgcac gaattcgact tcaagtccgg
     6781 tgcgggattt gatgtaagac gccagatcaa taaggggatc cggctgaccg gt
//
LOCUS       DAOPPA010000123         6827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12283_length_6827_cov_4.032930, whole genome shotgun sequence.
ACCESSION   DAOPPA010000123 DAOPPA010000000
VERSION     DAOPPA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12283_length_6827_cov_4.032930"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /gene="glgP"
                     /locus_tag="PKH54_05205"
     CDS             <1..1314
                     /gene="glgP"
                     /locus_tag="PKH54_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOC99321.1"
                     /translation="TWVSPEIDNLLDMYLGPRYKGHPEDPEVWQRVRSIPASEMWRVH
                     MTRKERLMFYVRKHLKKQMMRQGASAVSVAGVEDVLNPEALTIGFARRFATYKRAGLL
                     FQQPERLIELLTVKDRPVQIVFAGKAHPQDFPAKEIIKKVVHFTRDPRIRSRIVFLED
                     YDMNVARYMVQGVDVWLNTPRRPLEASGTSGMKAAVNGALNLSVLDGWWDEGYSPDCG
                     WQIGGHDVSDDIVEQDRIECEALFDLLEQEIVPLYYDRNHSGLPERWIEMMKNSISRL
                     GSQFNTARMVAEYTRQAYWPLHLQGIVGTAHEFKGFRDLAAWRGRIAEPWSGVWVRVV
                     GTDASEVVSGSVMTVRLAAGLGDLAPEEVRVEVWAGVLGSDDQIHDGRAVCATFVGMN
                     DGAAQFQARIQARDSGRHGFAARIIPVHPGVVALPTPPMVTWENA"
     gene            1363..1743
                     /gene="crcB"
                     /locus_tag="PKH54_05210"
     CDS             1363..1743
                     /gene="crcB"
                     /locus_tag="PKH54_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOC99322.1"
                     /translation="MRELLLVCLGGGIGTGARFLAGRALNSVAWSWLPVGTILVNIVG
                     SFLLSFIAVFSMRHGKLPDVVVLMLTTGVMGGFTTYSTFNHETLSMIQAGRWQLAVAN
                     VGVTFLMCGGAGLLGMFLASRIPA"
     gene            1918..2607
                     /locus_tag="PKH54_05215"
     CDS             1918..2607
                     /locus_tag="PKH54_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99323.1"
                     /translation="MRRKTIINWSDAHARLKEAIENKVTDPQMVGATEKGWSYVTFCS
                     SRAEPAHFLYDIDRIKTIARDNGFYLPGEIVRQHNKVLVIAHSRDHEPSQQLFGLWRF
                     LMEFEKRFDSTDKFPHHDFFGKFGGNFDRSEKGRVLAIYSNGGDDALMAVYESCEELA
                     AAIEVPGVTFEVGVSAGLSAIPRLLAGFDDPEYRATGADFYRINNPAEFEELLETARN
                     DFPKYLFHSPK"
     gene            complement(2826..3335)
                     /locus_tag="PKH54_05220"
     CDS             complement(2826..3335)
                     /locus_tag="PKH54_05220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC99324.1"
                     /translation="MASTFVILETTVGNIEIELYMKEAPVTCANFLEYVKANFYTGTI
                     FHRIIPGFMAQGGGFDVAKRQKPTRAPIVNEAGNGLKNVRTSVAMARTSDPNSATAQF
                     FINLADNGFLDRAESQDGVGYAVFGHVTQGMDFVDEAAKIPTVRNEISEAWPTKLVAI
                     RKASVIERP"
     gene            3610..5520
                     /locus_tag="PKH54_05225"
     CDS             3610..5520
                     /locus_tag="PKH54_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99325.1"
                     /translation="MRRAALLFVPIVFLVACGSSVNGTDNGVNIDVNVNYDNGGSDVD
                     NPPSDVNNQDTTGTPDIASDAVVDTMPQDIGEITRVITLQVDQQNPIPVAINQQFTFR
                     GQVKDANTGDPVPNPAVSFTITYVEDTEKAEEVLEYDSWITRPSTVGDAEGRVSSTFQ
                     AGETPKRLYTITVHSDGAVDQVFKILVQEMDCSCSAVTLTYDGVPGDGASYHLLAFDS
                     SHTCAELLAADELPAAAAEINHGDITTPAKIPCLTPGATYTVLVTAAETCAFAKGCAE
                     GVTVGAANDGENCGVGTVALDGIEVAINKQYDGKHKFTFTNVVPACTFSSSMDCANVG
                     DKTIGQQACCYLNSIQELFADDGTAFANAAADQAGLEGAGRTAAVNAVKEWVATKKPA
                     WAAKAASMGAMLRNVIVQTNMTSKLKIEAPDGEGNFQGSEDWRTFVLYWKMDCDPSDP
                     NYYNCGKFEISQTAIGGISYSPAISKSTFGGSLGIANNFSIAHHQINLNPGRLLVYVA
                     NKVAAVTLTGGYINASQELITVGAAADLKDAYKRWIACATMAADLASSAPAVTEAICN
                     AAVDAAMADITAAHNALVADSYLSLAGNGAYTDATCDGFADELSAGAYTGTWVRGAAE
                     IEMTGTFSGIDE"
     gene            5605..>6827
                     /locus_tag="PKH54_05230"
     CDS             5605..>6827
                     /locus_tag="PKH54_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HOC99326.1"
                     /translation="MEVREILLGDEAVALGALHAGISGAFGYPGTPSTEMFEYVEPLA
                     AEYGIHARWSANEKVAYEEALGMSFAGKRAIVSMKHVGLNVAADAFVNSGVTGVNGGL
                     VLMVADDPGMHSSQNEQDSRFYAKFAAVPCYEPKNQQECYDMVREAFDVSERLRLPIV
                     LRMVTRLAHSRADVVIGDRRPQNGLNPPTDKQQFTLLPTNARRNWAALVARQGEIIGE
                     SEASRFNRLELEGRDRKIGIIANGVACNYVREAFGGEVPHAMLSISRYPLPLGLIRKL
                     IEACESIFVFEEGYPLIEEAIAGINGIGKPLHGRSDGTVPATGELTPAIAMDVFGIGR
                     TGGIKSTLDHVPGRPPKLCQGCSHADSYTLIKEVLKDYPVKRTFSDIGCYTLGFYEPF
                     DGVHSCVDMGASVSMA"
BASE COUNT         1342 a         1967 c         2092 g         1426 t
ORIGIN      
        1 acctgggttt cccccgaaat cgacaacctt ctggatatgt atctggggcc aaggtacaag
       61 ggccacccgg aagaccccga agtctggcag cgcgttcgca gcattccggc aagcgaaatg
      121 tggcgcgtcc acatgacccg caaggaacgg ctgatgtttt atgtgcgcaa gcatctgaag
      181 aagcagatga tgcgccaggg cgcaagcgcg gtatcggtcg ccggtgtcga ggacgttctg
      241 aatcccgaag ccctgacgat tggtttcgcg cgccggttcg cgacgtacaa gcgcgcggga
      301 ctgttgttcc agcagcccga gcggttgatt gaactgttga ccgtcaagga ccggccggta
      361 cagatcgtgt tcgccggaaa ggcacatcca caggacttcc cggccaagga aatcatcaag
      421 aaggttgtgc acttcactcg tgacccgcgc attcgttccc gaatcgtgtt cctggaggac
      481 tacgacatga acgtggccag gtacatggtt cagggcgtcg atgtatggct gaataccccg
      541 cgccgcccgc tggaggcttc cggcaccagc ggcatgaagg ccgcggtcaa tggcgcgctg
      601 aacctgtctg ttttggacgg ctggtgggac gaaggataca gccctgactg cggttggcag
      661 atcggcgggc atgacgtgtc ggatgacatt gtcgaacagg atcgcatcga gtgcgaggca
      721 ctgtttgacc tgctcgagca ggaaattgtg ccgttgtatt acgaccgtaa tcacagcgga
      781 ttgcccgagc gctggatcga gatgatgaaa aattccatct cgcgtcttgg aagtcagttc
      841 aacacggcaa ggatggtcgc ggaatacaca cgtcaggcat actggccgct gcaccttcag
      901 ggcattgtcg gtacggccca tgaattcaag ggtttccgtg atcttgccgc ctggcgtggc
      961 cggattgccg agccgtggtc aggtgtctgg gtacgtgttg tcggaaccga tgcgtccgaa
     1021 gtcgtgtcgg gcagcgtgat gaccgtgcgg cttgccgcgg gacttggcga cctggcgccg
     1081 gaagaggtcc gggtcgaggt gtgggccggc gtgctgggtt cggacgacca gattcatgac
     1141 ggccgggccg tctgtgccac cttcgtcggc atgaacgacg gcgcggccca gttccaggca
     1201 aggatccagg ccagggactc cggacgtcac ggattcgcgg caaggattat tcccgttcat
     1261 ccgggcgtcg tcgccctgcc gaccccgccg atggtaacct gggaaaatgc ttgaggctcg
     1321 gctgagcccg cttttgaagc tggctcttaa gggtttggat taatgaggga acttcttctt
     1381 gtctgcctgg gcggcgggat tgggaccggg gcccgcttcc ttgccggccg cgccctgaat
     1441 tcggtcgcct ggtcctggct tccggtgggc accatactgg tcaacatcgt cgggtcgttc
     1501 ctgctttcct tcatcgcggt tttcagcatg cggcacggca agttgcccga cgtggtcgtc
     1561 ctgatgctga ccacgggcgt gatgggcgga ttcaccacgt attcgacctt taatcacgaa
     1621 acgctttcca tgatccaggc cgggagatgg cagctcgcgg ttgccaacgt cggggtcacc
     1681 tttctgatgt gtggcggcgc cgggcttctt ggcatgttcc tggcatcgag gattccggct
     1741 taatcttccg gcgtgcgttc gattcgagcg attctcgcaa cacccttgat tcatgactct
     1801 taatgccaaa ggatttggcc aggcaatcca ccggatgtaa tctatttgtg tggccggcgg
     1861 gaatacaccc cttccggcgc cgttgttcct tttggcagtc agcctgcagg agagccaatg
     1921 agaagaaaga caatcatcaa ctggtccgac gcacacgcaa gactgaagga ggcaatcgag
     1981 aacaaggtga ccgaccctca gatggtcggc gcgaccgaga agggctggtc ttacgtgact
     2041 ttttgcagtt ccagggccga gcccgcccat ttcctgtacg acatcgaccg aatcaagaca
     2101 atcgcccgcg acaacggttt ctaccttcct ggcgagatcg tgcgccagca caacaaggtt
     2161 ctggtcatcg cgcacagtcg ggatcacgag ccaagccagc agcttttcgg tttgtggcgc
     2221 ttcctgatgg aatttgaaaa gcggttcgat tcgaccgaca agtttccgca tcatgacttc
     2281 ttcgggaagt tcgggggaaa tttcgacaga tccgaaaagg gaagggttct ggcaatttac
     2341 agcaacggcg gcgacgacgc tcttatggct gtatatgaaa gctgcgagga actggcggcc
     2401 gccatcgaag tgccgggggt gacgttcgaa gttggcgttt ccgcgggtct ttcggcgatt
     2461 cccaggcttc tggccggctt tgacgatccg gagtaccgcg ccaccggggc cgatttttac
     2521 cgcatcaaca atccggccga gttcgaggag cttctggaga cagcccggaa cgattttcca
     2581 aagtatctgt tccactcacc gaagtgattt gcagacagac ggattttcat gcgcaggcgg
     2641 gcgaattctc attcgccctt acatgtacgg gcggattaca tccgcccgct gcggtcgagt
     2701 acagagtctc tgcgccctgg gcgggcgaat tctcattcgc ccttacatgt acgggcggat
     2761 tacatccgcc cgctgcggtc gagtacagag tccccgggcc tgggcgggcg aattttcatt
     2821 cgcccttacg ggcgttcgat gacgctggcc ttgcggatgg cgaccagctt ggtcggccag
     2881 gcttcgctga tttcgttgcg gactgtcgga atcttcgcgg cttcgtcaac gaagtccatg
     2941 ccctgggtca catgcccgaa aacggcgtaa cccacgccgt cctgtgattc ggcgcgatcc
     3001 aggaagccgt tgtcggccag gttgatgaaa aactgggccg tggcgctgtt cgggtcggat
     3061 gtgcgcgcca tcgccaccga agtgcggacg ttcttcagtc cgttgccggc ttcattcact
     3121 atcggggctc gggtcggctt ctgccgcttc gcgacatcga aaccgccgcc ctgggccatg
     3181 aatcccggga tgatgcggtg gaagattgtg cccgtgtaga agttcgcctt gacgtactcc
     3241 aggaagtttg cgcacgtgac cggcgcttcc ttcatataca gctcgatttc gatgttgccg
     3301 acggttgttt ccaggattac gaatgtgctc gccatgaaag cctccagaaa gaattaatgc
     3361 cgccagacgg ggctggcttg atgatacatc gcgcatgtcc aggccgtcct gtcgggtcga
     3421 ggcttaagca aacgcgaaag ccgggcccgg tatcggagcg ggccgcccgt gcgcacgcgc
     3481 aatataacag atttatgata attgctcttg ttattggctc gtttgctttc taaactcatg
     3541 ccaccccggg gtggcggcct ggttcccgga tcccggacgt ttttgtcgaa cttcgctttg
     3601 gaggtttcca tgcgtcgtgc agccctgctg ttcgtaccga tcgttttcct tgtcgcctgc
     3661 ggttcttccg ttaatggaac cgataatggc gtgaacattg acgtgaacgt caattacgac
     3721 aacggtggat ccgatgtcga taatccaccg tccgatgtca ataatcagga caccactgga
     3781 acaccggaca ttgcgtccga cgccgtcgtc gacacgatgc cccaggatat cggcgaaatc
     3841 acgcgcgtaa tcaccttgca ggtggatcag cagaacccga ttccggtcgc aatcaatcag
     3901 cagttcacct tccgtggcca ggtgaaggac gcgaacaccg gtgacccggt tcccaatccc
     3961 gcggtttcgt tcacgatcac ctatgtcgag gacacggaaa aggccgagga agtccttgag
     4021 tacgacagct ggatcacccg accatcgacg gtcggtgatg ccgaggggcg tgtttcgagt
     4081 actttccagg ccggggaaac cccgaagcgc ctttacacga tcacggttca ttcggatggg
     4141 gccgtcgatc aggttttcaa gattctggtc caggaaatgg actgcagttg cagcgccgtg
     4201 acgcttacct atgacggcgt gcctggcgat ggcgcctcgt atcatcttct ggcttttgat
     4261 tcttcccaca cctgcgccga actgctggca gccgacgaac ttcccgccgc ggcggccgaa
     4321 atcaatcatg gcgacatcac gaccccggcc aagatcccgt gcctgacccc cggggccacc
     4381 tataccgtcc tggttacggc agccgagacc tgtgcttttg caaagggttg cgcggaaggc
     4441 gttaccgtcg gggcggccaa tgatggcgaa aactgcggcg tcggcaccgt cgcgctcgac
     4501 ggcatcgagg tcgccatcaa caagcagtac gacggcaagc acaagttcac gttcaccaac
     4561 gtcgtcccgg cgtgtacctt cagttcgtcg atggactgcg cgaatgtcgg cgacaagacc
     4621 atcggccagc aggcatgctg ttatctgaat tcaatccagg aactgttcgc ggatgacggc
     4681 accgcgttcg ccaatgcggc agccgatcag gccggccttg aaggcgccgg ccgcacggcg
     4741 gccgtcaatg cggtcaagga atgggtcgcg acgaagaagc cggcctgggc cgccaaggcc
     4801 gcttcaatgg gtgcgatgct tcgcaacgtg atcgtccaga ccaacatgac gtccaagctg
     4861 aagatcgagg cgccggacgg tgagggcaac ttccagggca gcgaggactg gcggacgttt
     4921 gtcctttact ggaagatgga ttgcgacccg tcggatccga actactacaa ctgcggaaag
     4981 tttgagatct cccagacggc catcggcggc atcagctatt cgcctgccat ttcgaaatcg
     5041 accttcggcg gttccctggg cattgccaac aacttctcga tcgcgcacca ccagatcaac
     5101 ctgaatcctg gtcgtctgct ggtctatgtg gccaacaagg tcgccgcggt cacccttacc
     5161 ggcggataca tcaatgccag ccaggaactc atcaccgtcg gtgcggcggc cgatttgaag
     5221 gacgcctaca agcgatggat tgcctgcgcg acaatggccg ctgacctggc ttcttcggcg
     5281 cccgccgtta ccgaggccat ctgcaatgcg gcagtcgatg ccgcgatggc cgatatcacc
     5341 gcagctcaca acgccctggt ggccgacagc tatctgagcc ttgccggaaa cggggcctat
     5401 accgatgcga cctgcgacgg tttcgccgac gagctttcgg ccggggcgta caccggcacg
     5461 tgggttcgtg gcgccgccga aatcgagatg acgggcacct tcagtggtat cgacgaatag
     5521 tcaaatcgga gagtcccata tcaggaatca ggtcggcagt tgaaggtaat tcgctgacga
     5581 atcaacaatt caaggagagt tccaatggaa gttcgcgaaa ttctgctggg cgatgaggcc
     5641 gtcgcgcttg gcgcgcttca cgcggggatc agtggcgcct tcggttatcc gggaacgcca
     5701 tcaaccgaga tgtttgagta cgtggagcct ctggctgccg agtacgggat tcatgcccgc
     5761 tggtccgcca acgaaaaggt cgcgtatgag gaagcgcttg gtatgtcgtt cgctggcaag
     5821 cgggcgattg tcagcatgaa gcacgtcggg ctgaacgtgg cggcagacgc atttgtcaat
     5881 tccggcgtca ccggcgtcaa cggcggactg gtgctgatgg ttgcggacga ccccggaatg
     5941 cacagcagcc agaacgagca ggattcccgc ttctacgcga agttcgcggc ggttccctgt
     6001 tacgagccga agaatcagca ggaatgctat gacatggttc gcgaggcttt cgatgtgtcg
     6061 gagcgcctgc gcctgccgat agtgcttcga atggtcaccc gccttgccca cagccgcgcc
     6121 gacgtggtaa ttggcgatcg ccgcccgcag aacgggctga atccgccgac cgacaagcag
     6181 cagttcacgc ttctgccgac gaacgcccgt cgcaactggg ccgctctcgt ggcgaggcag
     6241 ggcgagatta tcggcgagtc cgaagccagc cgtttcaaca ggctcgaact ggaaggccgt
     6301 gatcggaaga tcggcatcat cgcgaacgga gtcgcctgca attatgtccg cgaggccttt
     6361 ggcggcgaag tcccgcatgc catgctcagc atcagtcgtt acccgctgcc gctgggcctg
     6421 attaggaagc tgatcgaagc atgcgaatcg atttttgttt ttgaagaagg ctatccgctg
     6481 atcgaggagg caatcgccgg tatcaacggt atcggcaagc cgcttcacgg ccggtccgac
     6541 ggcacggttc ccgcgaccgg tgaattgacg ccggcgattg cgatggacgt gttcggtatc
     6601 ggccgcaccg gtggcatcaa atcgacgctc gatcacgtcc ccggccgtcc gcccaagctc
     6661 tgtcagggat gcagccacgc cgacagctac acgctgatca aggaagtcct gaaggattat
     6721 ccggtcaagc ggacgttcag cgatatcggc tgctacactc tgggattcta cgagcccttc
     6781 gatggagtcc atagctgcgt cgatatgggc gccagcgtgt cgatggc
//
LOCUS       DAOPPA010000124         6799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12341_length_6799_cov_3.358689, whole genome shotgun sequence.
ACCESSION   DAOPPA010000124 DAOPPA010000000
VERSION     DAOPPA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12341_length_6799_cov_3.358689"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PKH54_05235"
     CDS             <1..1427
                     /locus_tag="PKH54_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99327.1"
                     /translation="RALLAPFFAAISMVPPLGLMYLASFLRELGHSVRLTRWNDVPEA
                     TARIHALIRERKPDVIGISAIVPECGTLRELMPKLQEIAPGVPVMVGGHLASANPLTT
                     LRIPGVSVIALREGERTAAELVEALASGRDPEGIPGSAVLRDDKVVLAPKRENLSTAE
                     LDELPFPAWDLLDFKELFSHRGMASVGIRPYMQVITSRGCPYRCIYCHATMGRMYRPR
                     SPEKVIEEIRILREQYDIHEFEIVDDCFNLDRKRMHRILDGLVAFGDPKLRLQFPNGL
                     RSDLLTREDVLLLKKAGTNFISFAVETASPRLQKYVRKNLDIDKAVNAISMAAGAGIF
                     CNGFFMLGFPTETAQEAKATIRLAVSTRLHEAMFFKVNPFRGTELFDRAVEEAHCNVD
                     GIDLNDLDFFTVSNNISQMSDEEFEKIYTSAYRRFYLNPVRIARILWHHPRRSQLFMY
                     MLQVVYKTGTALLEWARRARVNSV"
     gene            complement(1452..1523)
                     /locus_tag="PKH54_05240"
     tRNA            complement(1452..1523)
                     /locus_tag="PKH54_05240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Val,seq:cac)
     gene            1735..2295
                     /locus_tag="PKH54_05245"
     CDS             1735..2295
                     /locus_tag="PKH54_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOC99328.1"
                     /translation="MGRKPEGNGGRVIKRYPNRKLYDTAESRYITLNEIAALLREGHE
                     VSVFDSRTGEDITSVTLAQVLVGEEKNHRRTLPIQKMAALIHSGGEFIQKKLPVVTTI
                     REEAERKVHELLKHNSAEELKEILVSTHHAYEEVQRRADEQIQFVVSTVKSIAPLARD
                     ISELRSQVRELSERVAELEQKAGPNR"
     gene            2353..4275
                     /locus_tag="PKH54_05250"
     CDS             2353..4275
                     /locus_tag="PKH54_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC99329.1"
                     /translation="MGSGRVIEGRFELGDRVKRHEPGESFNAVDKSGGQDVVLRILPG
                     TFGDEARQTAFFERIQSVVKMNGKHLEKVLDAGVTEGGAPYVVTERLKGSPLDQIIGR
                     EAPLAVMRAVEICRQIAFGLGDASRAGCVHGRLSPRLVSVSAGKRGKGVEKVKITGYA
                     PWDLADSRAAFDFEVIAPEVILGAEPDIRSDCYAMGVILYSMLAGRSPFTAASREALT
                     LAHLNDRIDVPSVARGDKEIPPALDNLVMRLLEKNPDKRLGAPDIVLVHLESIRSGEQ
                     DSGIEPQDGENPSGVAAAADSLPDADSDLDEGIYFASGRRIKTTDFQSIKVTELMGSI
                     AGREVEPTAPLGRLDPVHAPAAPAGATAGAKPVPDASAVPAKAKGHSSTLAIVVVSGL
                     FFLAAVAVTGWFVLQYQTRKLELAAQSAPAAARVQTAEPVRQAAAIAPAPATVPATAP
                     DSQADQGTQASPTAGQMAAQPVAAQPVAASPNDAQPTVTPPAAASADAAQAVAPAQPA
                     TTKAQATAPVKTEPTDGGQAPKQARPVQAQASSTTAKPVQQAASGQATAPARKPQKTA
                     AVAAPKPGKDGKPTGSTDKKPAPAAAKPATASGGSSVDWFDGMDEKPAAKPAAKDKPK
                     KDSGKKASEPVLNWDE"
     gene            4279..5427
                     /locus_tag="PKH54_05255"
     CDS             4279..5427
                     /locus_tag="PKH54_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99330.1"
                     /translation="MKKIIVAAVGICLLIGTALPAAEPSAEEKEAFRLYKEAQAMMEL
                     SQYDQAIEHLNKAWGIFRIPQIAIMKAKALQSKGDLTTALMVLKGIVSDDERIRTRVG
                     AAIAELEAELAKPIEIDVTTSVDQVRMVIDKTSTFLIPGRITVTPGPHRIEYSAPGYR
                     TKTEERSFSAADKTLEVLMEEAGGKIVLTTDLDSFFGVVVRIDGRELMPRGSSLTPNR
                     TAPIEIAPGAHSFTCSKNGMPDYSGTFTLLSMESAEVHCKFSPVATTKKAGGPKAPMS
                     VPKNPLAWTFFGIGTGMVIAGTALTGWYWDLKISNPDANFKDESYEGYWGPAMAGVGI
                     AVAVMGFFIFKDKPKDARTAASTGPGKSFGFAVAPTRDGAAASFGMAF"
     gene            complement(5596..>6799)
                     /locus_tag="PKH54_05260"
     CDS             complement(5596..>6799)
                     /locus_tag="PKH54_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99331.1"
                     /translation="SLVTLCATLALISLVPAATVAQSIDEIVPTEVEVGKEFVDNFKA
                     NAKIGATLNFTNNHKFVGQLDGNAVNIGANVDTGYTLIRKGHDWRAQLKLMLSFAYGP
                     PLNRLQKSTDQFYFDTLYFYHPKGYEWIGPFARFTLDTVMLDGVSNTVEKNDYVLEGA
                     AAGDSPLKSGTDRFTLTRAFSPITFKEAAGIYARPISKPVIEVIFTFGFGASQVAMAG
                     SQYTIAAAEVGKITLRKLQPYEQVGLAGGLNLKGTYEKKIKMIYSAFCEFMVPFYPDP
                     PAGKGYGDLTNVDIGANLTFKLFKWATLDYVLKVVRQPQLVADWQVQNMLMLTLGYTW
                     DYDENQKKAAAAEAAKAEAEAAKNAQAAAAAPAEAPAAPAEVPAAEPAAEPAAEPAAE
                     PPAEAPAA"
BASE COUNT         1436 a         2033 c         2093 g         1237 t
ORIGIN      
        1 tgcgggcgct gctggcgcca ttctttgccg ccatctcgat ggttccgccg ctgggcctga
       61 tgtacctggc gtccttcctg agagagctgg ggcattcggt caggctgacc agatggaatg
      121 acgtcccgga agcgacagcc cgcattcatg cattgatccg cgagcggaag ccggacgtta
      181 tcggcatcag cgcgatcgtc ccggagtgcg gcacgcttcg ggagttgatg ccgaagcttc
      241 aggagatcgc ccctggtgtt ccagtcatgg tcggcggtca ccttgcgtcc gcgaacccgc
      301 tgacgacact gagaataccc ggcgtcagcg tgatcgccct tcgcgaaggc gaaaggacgg
      361 ccgcagaact ggtcgaggca cttgcctccg gacgcgatcc agaaggcata ccaggctcgg
      421 ccgtgcttcg tgacgacaag gttgtcctgg cccccaaaag ggaaaatctt tcgacggccg
      481 aactggacga actgccgttt ccggcatggg atctgctgga cttcaaggaa cttttctccc
      541 accgcggcat ggcttcagtc ggaatcaggc cgtacatgca ggtcatcacg tcccgcggat
      601 gcccgtacag atgcatttac tgccacgcca cgatgggccg catgtaccgt cccaggtcac
      661 cggaaaaggt aatcgaggag atccgaatcc tgcgcgagca gtacgacatc cacgagttcg
      721 agatcgtcga tgactgcttc aacctggatc gtaaacggat gcaccggata cttgacggac
      781 tggtcgcgtt tggcgacccg aagctgcggc ttcagttccc gaacggactg cgatcggatc
      841 tgctgaccag ggaagacgtc ctgctgctga aaaaggcggg gacgaacttc atatcgttcg
      901 cggttgagac ggcgtcgcca agactccaga agtacgtcag gaaaaatctg gacatcgata
      961 aggccgtcaa cgccatttca atggcagccg gggcaggcat cttctgcaac ggattcttca
     1021 tgctcggatt cccgaccgag actgcccagg aggccaaggc aaccatccgc ctggcggtct
     1081 cgaccagact gcacgaggcg atgttcttca aggtcaaccc gttccggggc accgagctct
     1141 tcgaccgcgc cgtcgaggag gcacactgca acgtggatgg aatcgacctg aacgacctgg
     1201 acttcttcac ggtcagcaac aacatctccc agatgtcgga cgaagaattc gaaaagatct
     1261 acacttccgc gtaccggcgc ttctacctga accccgtcag gatcgccagg atactctggc
     1321 accacccgcg acgaagccag ctcttcatgt acatgctgca agtcgtttac aagaccggca
     1381 ccgcacttct ggaatgggcg cgcagggcga gggtcaacag cgtctgaaag cacggcggca
     1441 ttgttccggg gtgggcgata ctgggattga accagtgact tccaccgtgt gaaggtggca
     1501 ctctcccgct gagttaatcg cccgggaaaa aagcgagcaa agaataggtc aacagcccct
     1561 tcctgtcaat acgcccgacg atcgcgccct ggccggaacc aaaaaagtcc tttctataat
     1621 aggttgtttg aattctaata tgtataatgc ggccttcccg gcattcatcc agcgactgca
     1681 actgactgcg gacgcgccgg gattgacgcg cgggaaacca caaggagcct ctcgatgggc
     1741 aggaaaccgg aaggaaacgg cggaagggtc atcaaaaggt accccaaccg caagctttac
     1801 gacacggccg aaagccgtta catcaccttg aacgagatcg ccgcccttct tcgtgagggc
     1861 cacgaagtct cggtcttcga cagcagaacc ggcgaggaca tcacctcggt tactcttgcc
     1921 caggtgcttg tcggcgagga gaaaaaccac agaagaaccc tgccgatcca gaagatggcc
     1981 gccctgattc attccggcgg ggagtttatt cagaagaaac tgccggtcgt gaccaccatc
     2041 cgggaggaag ccgaacgcaa ggttcatgaa ctcctgaagc acaactcggc cgaggagctg
     2101 aaggaaatcc tggtcagcac ccaccacgcg tacgaggagg ttcagcggcg cgcggacgag
     2161 cagatccagt tcgtcgtaag cacggtcaag agcatcgcgc cgcttgcccg cgatatttcc
     2221 gaactgcgca gccaggttcg ggaattgagc gagcgcgttg ccgagttgga acagaaggcc
     2281 ggcccgaatc gataagagtc ccgcatatcg aattcagcgg cgacggcgga tgtaggtcgt
     2341 acggaggatc cgatgggaag cggtcgagtt attgaaggac ggtttgaact tggcgaccgt
     2401 gtcaaaaggc acgagccggg cgaatcgttt aatgccgttg acaaaagcgg cgggcaggat
     2461 gtcgttctgc ggattctgcc ggggactttc ggggacgagg cccgtcagac cgcctttttc
     2521 gaaagaatcc agtcagttgt gaagatgaat ggaaagcacc ttgaaaaggt gctggacgcc
     2581 ggcgtcaccg agggtggggc accatacgtc gtcaccgaaa ggctgaaggg ttcccccctg
     2641 gatcagataa tcgggcggga ggctccgctc gcggtgatgc gcgccgtcga aatatgcagg
     2701 cagattgcct tcggacttgg tgacgcctcc cgggccggat gcgtccacgg gcgactgtcg
     2761 ccaaggctgg tgtcggtatc tgccggcaag cgcggcaagg gcgtcgagaa ggtcaaaatc
     2821 accggatacg cgccctggga tcttgccgac agccgtgccg ctttcgattt cgaggtaatc
     2881 gcccccgagg taattcttgg cgccgagccg gacatcaggt cggactgcta tgcaatgggg
     2941 gtcatcctct attcgatgct cgccggaagg agcccgttca ccgcggccag ccgcgaagca
     3001 cttaccctgg cgcacctgaa cgacaggatc gatgtccctt cggtcgccag gggcgacaag
     3061 gaaatcccgc ccgcgctgga caacctggtg atgcgccttc ttgaaaagaa tcccgacaag
     3121 cggcttgggg ctcccgacat cgttctggtt caccttgaat caatcagatc cggtgaacag
     3181 gattcgggga tcgagcccca ggatggcgaa aacccgtccg gggtggccgc agcggccgat
     3241 tctcttccgg atgccgacag cgaccttgac gaaggaattt acttcgcgtc aggaagacga
     3301 atcaagacta ccgacttcca gtcgataaag gttaccgagt tgatgggatc aatcgccggg
     3361 cgggaagtcg aaccgacggc gccacttggg cggctggatc ctgtccacgc cccggcggcc
     3421 ccggcaggcg ccacggccgg agccaaaccg gttccggatg catccgctgt cccggcgaaa
     3481 gcgaaagggc attcatcgac gctggcaatc gtggtggttt ccggcctgtt cttccttgcc
     3541 gctgtcgccg tgaccggctg gttcgtcctg cagtaccaga cacgaaagct ggaactggcc
     3601 gcccagtcag cgccggcggc agcccgggtt caaacggccg aaccggttcg gcaggccgcg
     3661 gcgatcgccc cggctcccgc aacggttccg gccacggccc ccgacagcca ggcggaccag
     3721 ggcacgcagg cgtccccgac ggctggccag atggcggccc agcctgtggc ggcccagcct
     3781 gtggcggcaa gcccgaatga tgctcagcct acggtgacgc cgcccgcagc ggcatcggcc
     3841 gacgcggctc aggcggtggc accggcacag ccggccacga ccaaggccca ggcgaccgcg
     3901 cctgtcaaga cggaaccgac cgacggcggc caggcgccga agcaggcccg tccggtgcag
     3961 gcgcaggctt cctcaacgac ggcaaagcct gtgcagcagg ccgcttcggg ccaggcgacc
     4021 gccccggcca ggaagccaca gaagaccgct gcagtcgccg cgccaaagcc aggcaaggat
     4081 ggaaagccga ccggttcaac cgataaaaag cccgctccgg ccgctgcgaa gcccgcgacg
     4141 gcgtccggcg ggtcttccgt tgactggttc gacggaatgg atgaaaaacc ggcggcaaag
     4201 ccggcggcaa aggataaacc gaagaaggac tccgggaaaa aggccagcga acctgtcctg
     4261 aactgggatg aatgacaaat gaaaaagatc atcgttgccg cagttggaat ctgtcttttg
     4321 atcggcacgg cacttccggc cgccgagcct tccgccgagg agaaagaggc cttccgcctt
     4381 tacaaggaag cgcaggcgat gatggagttg agccagtacg accaggccat cgagcacctg
     4441 aacaaggcct ggggtatatt ccggattccc cagattgcga tcatgaaggc aaaggccttg
     4501 cagtccaagg gtgacctgac gaccgccctg atggtcctga agggcatcgt ttccgatgac
     4561 gaacggatca ggacaagggt cggtgcggca atcgcggaac tggaggccga actggcgaag
     4621 ccgatcgaga tcgacgtgac gacttcggtc gatcaggtgc gcatggtgat cgacaagaca
     4681 tcgacgttcc tgattccggg caggatcacg gtaacgcccg gaccgcaccg gattgaatac
     4741 tcggccccgg gttaccggac aaagaccgag gaaaggtcat tttccgcggc tgacaagacg
     4801 cttgaagtcc tgatggaaga ggccggtgga aagatcgtgc ttacgaccga cctggattcc
     4861 ttcttcggag ttgttgtccg gattgacggc cgcgaactga tgccgcgtgg aagttcgctg
     4921 accccgaaca ggacggcgcc gatcgagatt gccccgggtg cccattcatt cacttgttca
     4981 aagaatggca tgccagacta ttccggcacg ttcacgctgc tttccatgga atcggccgag
     5041 gttcactgca agttttcgcc cgtggccacg acaaagaagg ccggcggccc caaggcgccg
     5101 atgagcgttc cgaaaaaccc gcttgcctgg acgttcttcg gaatcggaac gggcatggtg
     5161 attgccggta ccgcccttac cggctggtac tgggatctca agataagcaa ccctgatgca
     5221 aatttcaaag acgagagcta tgaaggctac tggggtccgg caatggccgg cgtcggcatc
     5281 gccgtggccg tgatgggatt cttcatcttc aaggacaagc cgaaggacgc ccggactgcc
     5341 gcttcaaccg gccccgggaa gtctttcgga ttcgccgtcg ccccgacccg ggacggcgcc
     5401 gccgcttcct tcgggatggc tttctgagac acgcatcacc cgacagtata ttttttccac
     5461 gccaatcctc cgacccttga tttgccccac cggcctggcc gacatccccg cgacttttca
     5521 tgccaggcgt gttcacaaac gtcggaatct gaagaaaatg aagtctggtc agcaggccgg
     5581 gacagtcgtg acaaatcagg cggcgggggc ctctgcgggc ggttcggcag ccggttcagc
     5641 ggccggttcg gcggccggtt cggcggctgg cacctcggcg ggggccgccg gtgcttccgc
     5701 cggggccgcg gccgcggcct gggcgttctt cgccgcttcc gcttcggcct tggcggcttc
     5761 ggccgccgcg gccttcttct ggttctcgtc gtagtcccag gtgtatccaa gcgtcagcat
     5821 cagcatgttc tgaacctgcc agtcggcgac caactggggc tggcgaacca ccttcaggac
     5881 gtagtccagc gttgcccact tgaacagctt gaatgtaagg ttcgcgccga tatcgacgtt
     5941 cgtcaggtca ccgtagccct tgcccgccgg cggatccgga tagaacggaa ccatgaattc
     6001 acagaacgcc gaatagatca tcttgatctt cttttcgtac gtacccttaa ggttaaggcc
     6061 gcctgcaaga ccgacctgct cgtacggctg cagcttcctc agggtgatct ttccgacctc
     6121 ggccgcggca attgtgtact ggctgccggc catggcaacc tggcttgccc cgaagccgaa
     6181 cgtgaagatg acctcgatca ccggcttgga aatcggcctt gcgtaaatac cggccgcttc
     6241 cttgaaggtg attggtgaaa acgcgcgtgt aagggtgaac ctgtcggtgc cggacttaag
     6301 cggactatcg ccggcagccg caccttcaag cacgtaatcg tttttctcga cggtgttgct
     6361 gacgccgtcg agcatgacgg tgtcaagcgt gaacctggca aaaggaccta tccattcgta
     6421 acccttcggg tgatagaaat agagggtgtc gaaatagaac tggtcagtgg acttctgaag
     6481 acggttcagc ggcggcccat acgcgaagga cagcatcagc ttcaactggg cgcgccagtc
     6541 gtggcccttg cggatcaagg tgtagccggt atcgacgttg gcgccgatat tgacggcgtt
     6601 gccgtcaagc tggcccacga acttgtgatt gttcgtgaag ttcagggtcg cgccaatctt
     6661 ggcgttcgcc ttgaagttat cgacgaattc ctttccgacc tcgacctcgg tcggcacaat
     6721 ctcgtcgatt gactgcgcca cggttgccgc gggcaccagg gaaataagcg ccagcgtcgc
     6781 gcacagcgtc accagtgag
//
LOCUS       DAOPPA010000125         6762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12429_length_6762_cov_4.074102, whole genome shotgun sequence.
ACCESSION   DAOPPA010000125 DAOPPA010000000
VERSION     DAOPPA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12429_length_6762_cov_4.074102"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKH54_05265"
     CDS             complement(<1..279)
                     /locus_tag="PKH54_05265"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOC99332.1"
                     /translation="MAENHDYFMAFALDAARAAGDRGEVPVGAALVINGHVESDHNRT
                     IEFGDPSAHAEALVLSRAARAGLDLGAATLYVTLEPCIMCAGAIVLSRI"
     gene            531..1862
                     /locus_tag="PKH54_05270"
     CDS             531..1862
                     /locus_tag="PKH54_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC99333.1"
                     /translation="MACIPQIIRSVAKQLGPKPLHMDIMSGAETTFVDFRNQAWFMAA
                     RLVRLPPVDDDGVALIGFSGVQALVLEAACQTASLVPFSIAPSIPADEILAAVLICRC
                     RWFILCDDPTFDAGLVRTTLEPLSHVATEIPIERLRPPPDIGEDELVAILEDPEVERR
                     ISVIGPRHAACGLAHVTPDGRTQVVAISHEGLGSLCESLRTELMADESDVWTSISGLE
                     SPFARIVCWYCCLLSGGTYTVAPLGRSPVEALFLTQPTIAVCDSVGAADIESGLISEL
                     QLLEGARGRLTRWGFHRARARTAAGTESHGMLDALADLAVDRLVRDIPGGSLRTILCD
                     GMNDPAASRTTFQTAGIDLWEISAPASACCFLAARRPGCFREGSVGKPLPVTSLFVSP
                     RGELMASGPAIMVSEMCLPPAECQDLDHGNLKTGLRGRVDDEGFVFIDGRL"
     gene            complement(1951..3576)
                     /locus_tag="PKH54_05275"
     CDS             complement(1951..3576)
                     /locus_tag="PKH54_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99334.1"
                     /translation="MERIETNRKSWCGAAVIIAMALFVGLPGCGGKVCPPGTVREGDF
                     CRRVKDDVPIEKDVVIVIDAGNDSDSQDVVSDPGNDREEPTDVVLDPGGMDLVEPEDT
                     GNPDKYPAGYIGMACNTAAVCRNDGWPDGSCLSWPRGFCAMPGCQDGVACPEGTTCMN
                     LAAGANGAYCTVTCQQASDCRDTVDLLGNKYACKLVPDPSNIFKSICYMEVGEASEVG
                     GPCQAHEHCQGAMGCLPNFDGGYCAVLSCDASNPCPEGTACTRLQGRGVCLKSCVDSQ
                     DCGHSVACQDGDEGCVGGSRQLSRACIDMKSALTGQTMKVCGSGTVGKSIGEQCLNET
                     ECKSGKCNVSYTGTCTDFDFTGRRCTTDADCAITKAICRQSSLQTFGFCTANCGTTTC
                     TVDQSICVMTLADEDSLKGLCLPRCVPSNPEDPFSESDCWEEAGLRCMYGDTKFQPGV
                     RSCVRIDPGDPGTNCRQDDECYSSNCIGFTGSTDGFCTMPCLADDLCPFPTICETVGG
                     SRLCMKRCMATPDCPGTMKCVTSTVGDYCAPAD"
     gene            3778..5322
                     /locus_tag="PKH54_05280"
     CDS             3778..5322
                     /locus_tag="PKH54_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOC99335.1"
                     /translation="MRFNCTNCNKIYEIDPGLIQGNAVMFTCRKCGQQLVIRKSDRPE
                     DAVIPAGKPGDRIKSFTQEISKVTKGKISALTTPHALRRENPPAWYVTAMGRRQGPLG
                     DLALIDLLSSGKILPATYAWRKSMTEWVRIKDIPELADVLAEAIRKNPEVGARINDKS
                     SLSAARVRSRSDVADLDSSVHRQEVPSGSLADDLYEGLGSGPIDPRNSTILQHEESPL
                     EDDEQPSRDPARAATVPHMRSIDWGEEIQDTLRHQTVKTRPFKRHDTAPINLALLRRE
                     RLNTRIALIVMISLISLAAIAAGVVVAIHFIDSGTSISHQAAIPARPAAARRNFDSVP
                     ADGPAAVPVREPEASPPVPAPTPVPTQEPPAQQQPPTQQEPTAQQQPPAQQQPPAQQQ
                     PPAQQQPPAQQQPPTVRLPLPSVGSTVVVDSGDIGLDELKSFVASQIPAISGCRSKLI
                     HQTDASIALELSFTLATSGRTEKVLARAVGMDEPRLTGCVEQVVSSWEFDQRSYPVSF
                     SGTLAL"
     gene            5460..>6762
                     /locus_tag="PKH54_05285"
     CDS             5460..>6762
                     /locus_tag="PKH54_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99336.1"
                     /translation="MTRFKTLAIILVLASTFGPGCSGSKVQVTPQITISPTVTNIQMG
                     ATYNIRIANSGDGDLIVSGFEIGGDTCTGGGFAFSVDLGEAELPVTIAPPGSEGDVNP
                     MWFDIVVRHDDPSQSCPATATLTISSNDPTQPRMVLQLTRTSTEPNIIAEPNPLDLGF
                     VPVGTIPASDYILVQNTGLDDLEITKLEVIPLSEGFSFTWPCVRNPDTSVTAPWGLDP
                     DSRITVSMVANPADRLVLDGDVCLEPLIVKPAESLSIPVFYTAIAPNPAKAEFRFHSN
                     DPDYDSTKGDAYTVTVQANMSGPCIQVLPNPIEFGSNIATTAVKGVPVVITNCGDEPL
                     EITSIRKEGDNCAEFSFDLTGTGEFDSTHPLVIQPGVSISIIAKYTPVDISPKDSENM
                     FIPDECLMVVDNNSARQSVEIPMSGVGTEAECAVCQFVMMEG"
BASE COUNT         1438 a         2102 c         1876 g         1346 t
ORIGIN      
        1 gatccgcgaa aggactatcg cgccggcgca cattatgcac ggctcgagag tcacatacag
       61 ggtggccgcc cccaggtcga ggccggccct ggccgcgcgg ctcagaacca gcgcctcggc
      121 atgggccgat ggatcgccaa actcgatcgt ccggttgtga tccgattcga cgtgcccgtt
      181 aatcacaagt gccgccccga ccggcacttc accgcgatca ccggccgccc gggcggcatc
      241 gagggcaaac gccatgaagt aatcatggtt ttcagccaag ccgccaccat ccccgcgcat
      301 gcaaagaacg cgcccaggcc cacagcgcat tgccagtcac ggcgcaacca tccccgcgca
      361 tgcaaagaac gcgccccaaa aagccggaag aatttatcag aggcgacttg aaacgtcaac
      421 agaaccttgc acgggtcttg atgagtgttt cgaaccagcg aagcgaatcg aatgggcatt
      481 cgtcgagcgt gggataacag gtcgacggcg gtacgtggta taagacggcc atggcctgca
      541 ttccacagat aatacggtcg gtcgcgaagc agctcggccc gaagcccttg cacatggata
      601 tcatgtccgg cgccgagact acctttgtcg atttccgaaa ccaggcatgg ttcatggcgg
      661 ccagactggt tcgccttccg ccggttgacg acgacggggt cgccctgatc ggattttccg
      721 gcgtccaggc gcttgtgctg gaggctgcgt gccagaccgc cagcctggtt ccgttctcaa
      781 tcgcgccgtc aattcccgcc gacgaaatcc ttgcggcggt tctgatctgc cggtgcagat
      841 ggttcatctt atgcgacgac ccgacgttcg acgccggcct tgtcaggaca acacttgaac
      901 ccctgtcgca tgtcgccacc gaaatcccga tcgaacggct tcgcccgccc cccgatatcg
      961 gcgaagacga gcttgtggcg attcttgaag atcctgaagt tgaaagacgg atttccgtta
     1021 ttggcccaag acacgcggcg tgcggactcg cccacgtgac gcccgacggc agaacccagg
     1081 ttgttgcgat ttcccacgaa ggcctgggca gcctttgcga atcgcttcgc acggagctca
     1141 tggctgacga aagcgatgtc tggacttcga tttccggact cgagtcgcct tttgccagga
     1201 tcgtctgctg gtactgctgc cttctttccg gtggaacata cacggttgcc ccattggggc
     1261 gatcccctgt cgaggcactc ttcctgacac aaccgacaat cgccgtctgc gattccgtcg
     1321 gcgccgccga cattgaatca ggactgatct ccgaactcca acttcttgaa ggtgccaggg
     1381 gcagactgac cagatggggt tttcacaggg caagggccag aaccgccgcc gggacggaat
     1441 cgcacggaat gctggacgct cttgccgatc tggccgtcga ccggctggtt cgcgatatcc
     1501 cgggcggctc ccttcggaca atcctttgcg acggaatgaa tgatccggca gcttccagga
     1561 cgaccttcca gacggcgggc atcgacctgt gggaaatctc ggcaccggcg tccgcctgct
     1621 gcttcctggc tgcgcgccgt cccggctgtt ttcgtgaagg ctcggtcggc aagccacttc
     1681 cggtcacctc gctcttcgtt tccccaaggg gggaactgat ggcttcgggg cctgcaataa
     1741 tggtttccga aatgtgcctt ccccccgccg aatgccagga tcttgaccac ggcaatctta
     1801 agaccggact tcgcggaagg gtcgatgacg aaggattcgt tttcatcgac ggaagattgt
     1861 agcctggatc gcaggccgga ctccccgaca tgcatcgaca tcaagacgtt caatatgcaa
     1921 cgggtctggc aaaggtaagc cgaaactgaa tcaatcggcc ggtgcgcagt agtcgccaac
     1981 cgtgctggta acgcacttca tggtaccggg acagtccggg gtcgccatgc atcgcttcat
     2041 gcacagcctt gatccgccaa ccgtttcaca gattgtgggg aagggacaca aatcatcggc
     2101 caggcacggc atcgtgcaga acccgtcggt actgccggtg aatccgatgc agttcgagga
     2161 atagcactcg tcgtcctgac gacagttcgt tcccggatca ccaggatcaa tcctgacaca
     2221 ggaccggacc ccgggctgga acttcgtgtc tccgtacatg cacctcagac ccgcctcctc
     2281 ccagcagtcg ctttcgctga aaggatcctc cggattcgac ggaacgcacc tgggaagaca
     2341 caatcccttc agactgtcct cgtctgcaag ggtcattacg caaattgact gatccaccgt
     2401 gcatgtcgtc gtgccgcaat tcgccgtgca gaatccaaaa gtctgcaaac tgctctggcg
     2461 gcagatggcc ttggtgatcg cgcaatcggc gtctgtcgtg caacgccgtc cggtgaaatc
     2521 gaagtcggta caggttccag tgtaggacac gttgcacttc ccggacttgc attcggtttc
     2581 gttaagacac tgttcaccaa tcgacttgcc aactgttccg gaaccgcaga ccttcattgt
     2641 ctgcccggtc agcgcgctct tcatatcgat acacgcgcgg gaaagctgac gacttccgcc
     2701 gacacaaccc tcatcgccgt cctgacaggc gaccgaatga ccacaatcct ggctgtcaac
     2761 acacgacttc aggcaaactc cgcggccctg aagcctggta caagccgtgc cctccggaca
     2821 tggattcgaa gcgtcgcaac tcagaaccgc gcaataaccg ccatcgaagt tcggaagaca
     2881 ccccatggcg ccctggcagt gctcgtgggc ctggcaggga cccccaactt cgctggcctc
     2941 gccgacttcc atgtaacaga ttgatttgaa aatgtttgac ggatccggaa caagcttgca
     3001 ggcatatttg ttgccaagca aatccacggt gtcgcggcaa tcggaagcct gctggcacgt
     3061 caccgtgcaa tacgcgccgt tggcgccggc agccaggttc atgcaggtcg tcccttcagg
     3121 acatgccacc ccgtcctggc agcccggcat ggcgcagaaa ccacgcggcc aggacaggca
     3181 cgaaccgtcc ggccagccat cgttcctgca gacggcggcc gtgttgcagg ccatgccgat
     3241 gtaaccggcc ggatacttgt cggggttacc ggtatcttct ggctcgacaa gatccatgcc
     3301 gccaggatcc agcacgacat cggtcggttc ttcacgatcg ttgccaggat ccgaaacgac
     3361 atcctggctg tcggaatcgt tgccggcgtc gatgacgata accacatcct tctcgatcgg
     3421 gacgtcgtcc ttgaccctgc gacagaagtc gccttcgcgt accgttcccg ggggacagac
     3481 ctttccgcca caacccggaa gaccgacgaa cagggccatc gcaatgatca cggccgcgcc
     3541 gcaccaactc ttccgattgg tctcaattct ttccatcgac aaaccaccag cactcaagca
     3601 gcgcggttat gatattcaaa attcttactt attgcgacgt gaaaatttct tgtcacaagc
     3661 cacttaacaa tcatataaac cccttgaacg aaagaatttt cagcaacccg cccttcaact
     3721 tggaacgggc gtccagctgg tgctagaatc cgtgcctgcg gaggcttttt tgccgaaatg
     3781 agattcaact gtacaaactg caacaagatt tacgaaatcg atccaggcct gatccagggc
     3841 aacgcggtca tgttcacctg ccgcaagtgc ggacagcaac tggtaatcag gaaaagcgac
     3901 aggcctgagg atgccgtcat ccccgccgga aagcctggcg accggatcaa aagcttcacc
     3961 caggaaattt caaaggtaac gaaaggcaag atatccgcgc tgacgacgcc gcacgccctg
     4021 cgtcgcgaaa acccgcctgc atggtacgtt accgcgatgg gccggcggca gggtcccctt
     4081 ggtgaccttg cgctgattga tctgctgtcg tcgggcaaga ttcttccggc cacgtacgcc
     4141 tggcgaaagt ccatgaccga atgggttcga atcaaggata taccggaact tgccgatgtc
     4201 ctggccgagg caatccgcaa gaatccggaa gtcggtgccc ggatcaacga caagtcaagc
     4261 ttaagtgcgg cccgagtacg ctcgagaagc gacgtcgccg atcttgattc gtcggttcac
     4321 agacaggaag tcccttcagg ctcgctcgcc gacgaccttt atgaggggct tggatccggc
     4381 ccgatcgatc cgagaaattc aacaatcctt cagcacgagg aatctccgct ggaggacgac
     4441 gaacagcctt caagggatcc tgcgcgtgcc gcaacggtgc ctcacatgcg ttcgattgac
     4501 tggggcgagg aaatccagga cacgctgcgt caccagactg tcaagacgcg gcccttcaag
     4561 cgtcacgaca cagcaccaat caatctggct cttcttcgcc gtgaacggtt gaacacccga
     4621 atcgcgctga tcgtaatgat cagcttgatt tcgctggccg caatcgctgc cggcgtggtt
     4681 gtggcgatac atttcatcga cagcgggaca tccatttcgc accaggcggc tattccggcc
     4741 aggccggccg cggcccgcag gaacttcgac tccgtcccgg cagacggccc ggctgcggtt
     4801 ccggttcgtg aacccgaggc cagtcccccc gtcccggcgc cgacgccagt tcccacccag
     4861 gagccgccgg cccagcagca gccgccgaca caacaggaac caacggctca gcagcaaccg
     4921 ccggcccagc agcaaccgcc ggctcagcag caaccgccgg ctcagcagca accgccggct
     4981 cagcagcaac cgccgacggt tcggctgcca cttccttcgg ttggttcaac tgtggttgtt
     5041 gacagcggcg acatcgggct cgacgagttg aaatcgttcg tcgcatctca gattcccgct
     5101 atatcgggat gccgttcgaa gctgattcac cagaccgacg catcgattgc gctggagctt
     5161 tcattcaccc tcgcgacctc cggcaggact gaaaaggttc ttgcgcgtgc ggtggggatg
     5221 gacgagccac gattgaccgg atgcgtcgaa caggtcgtct cgagttggga attcgaccag
     5281 cgttcctacc ctgtcagctt ttcaggcacg ctggcgcttt gattcgaggt ttgtcccgga
     5341 cagcggaagt ccggtgcagg ccactggaat tgttgccagt gcatgccttt tgagcctgtc
     5401 tttattcgca tttattttgt agaatccgct ggaagttttt ccggagcccg aggtcagaca
     5461 tgacgagatt caagacgctt gcgattattc tggtgcttgc atccactttc ggccccggtt
     5521 gttccgggtc caaggttcag gtaaccccgc agattacaat cagtcccacg gtaacgaaca
     5581 tccagatggg cgcaacctac aacatcagga ttgcgaactc cggcgatggc gaccttatcg
     5641 tctcgggatt tgagatcggc ggcgacacat gcaccggcgg cggttttgca ttttcggtcg
     5701 atcttgggga agccgagctt ccggtaacga ttgcaccgcc cggatcggaa ggcgacgtga
     5761 acccgatgtg gttcgacatc gtcgttcggc atgacgatcc ttcgcagagt tgccccgcga
     5821 ccgcaacact tacaatcagt tcaaacgatc cgacgcagcc caggatggtt ctccagctta
     5881 cgcggacttc gacggaaccg aacatcatcg ccgagccgaa tccgctggat ctgggattcg
     5941 taccggtcgg aaccattccg gcgtccgatt acatcctcgt tcagaacacc gggcttgatg
     6001 atctggaaat cacgaagctt gaagtaattc ctctttcgga aggtttctca ttcacatggc
     6061 catgcgtccg caacccggac acttcggtca cggccccctg gggacttgac ccggattccc
     6121 gcataacggt ttccatggtc gcgaatccgg ccgatcgcct ggttctggac ggggacgtct
     6181 gcctggagcc tctgatcgtc aaacccgccg agtcgctctc gattccggtg ttctacacgg
     6241 caatcgctcc gaaccccgca aaggcggaat tccgtttcca cagcaatgat ccggactatg
     6301 acagcacgaa gggtgacgca tacacggtca cggttcaggc caacatgagc ggcccgtgca
     6361 tccaggttct gccgaatccg atcgaattcg ggtcgaacat tgcgacgacc gccgtcaagg
     6421 gagttccggt cgtgatcacg aactgcggcg atgaacccct tgaaatcaca tccatccgga
     6481 aggaaggcga caactgcgcg gaattcagct tcgaccttac tggaacgggc gaattcgaca
     6541 gcacgcaccc gctggtcatc cagcccggtg tttccatcag catcatcgcg aaatataccc
     6601 cggtcgatat cagccccaag gactcggaaa acatgttcat tccggacgaa tgcctgatgg
     6661 tggttgacaa caattccgcc aggcagtcgg tcgagatacc gatgtccggc gtcggcacgg
     6721 aagcggaatg tgccgtatgt cagtttgtca tgatggaagg cg
//
LOCUS       DAOPPA010000126         6730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12501_length_6730_cov_3.801798, whole genome shotgun sequence.
ACCESSION   DAOPPA010000126 DAOPPA010000000
VERSION     DAOPPA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12501_length_6730_cov_3.801798"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKH54_05290"
     CDS             complement(<1..365)
                     /locus_tag="PKH54_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOC99337.1"
                     /translation="MNGPEILTVAQAMNLSSGFLAKKGSASPRLDAQILTAHALGIRR
                     LDLYLAPDRPLSELERDTLRKFLVRRSAGEPVAYIVGFREFFGLSFRVTPAVLVPRPE
                     TEAIVDAALNLLDVMAGEG"
     gene            complement(393..1421)
                     /gene="dusB"
                     /locus_tag="PKH54_05295"
     CDS             complement(393..1421)
                     /gene="dusB"
                     /locus_tag="PKH54_05295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOC99338.1"
                     /translation="MPVSEFRRLVVEGRLVNAPLDGYSDPAWRTICLRHGATLCYTEM
                     IPAIALVFGASDAMRRLRRADGESILAVQIEGAKPDIMVEAARIAVNAGADIIDINAG
                     CPSQRVTNSGAGSALLSDLDLLHEIARAVKGAVDVPVSMKVRSGPTQSNIVVDEISAM
                     VNDLDLAAVTIHPRTRNQAYRGKADWSLIARMKSSCRIPVIGNGDIRSPADALRMMET
                     TGCDAVMIARGAIGNPWLFSQTRACLAGQEAPAPVNRQEWERTVWDHFNLFLDYLDGF
                     EGIAARLFRKHLARYTRGMAGATEFRRGLPHINDRAGLRMALERLLAAGEEAGGFVFP
                     MPRPDDTF"
     gene            complement(1513..4053)
                     /locus_tag="PKH54_05300"
     CDS             complement(1513..4053)
                     /locus_tag="PKH54_05300"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99339.1"
                     /translation="MKRFTSIVLPSVFFCMLLASCEGSDPRKIGEFCESEADCESGLC
                     YDSQCLNPDGDFDLDGIKNGIERNITGTSYGLADTDGDGINDGTEVGNDVNSPLDGDG
                     DGIIDALESRLPTADPDGDCLPDEFDAQNNEFSENPAKVAELNCSRAGVCGLAFDKVT
                     ATCAQGVAACDYSQVPSFEETEATCDGLDNDCDGKADIGLQPPSQPACSHKGICAGRQ
                     EVIFRVCLRGEWVCEYSGITGWEPNESSCDSIDNNCDGETDEGLVGQDCFNRNEYGKC
                     AGTTACNEFKGVTCIGQTPAAEICNGIDDNCNGSTDEDLVGIECEIVNEFGTCTGETV
                     CDIQTGGADCDAMTPAQEICDTIDNDCDGMTDEDFICDRTSTIMMKVFGASLPPPPFK
                     SSATGEGYQALSGASVSFYKDSCPPETELSAAWTVTTDDEGKSEIPIEPGLWCIQVSA
                     EGYQAMTTDVLFLEPGTVIPIEMILLLNGMTQAQLSMCGRVLEYDDYVVTTSGSTEPA
                     SGSPVPAALITLVDMTGSTEIATTTSDSNGYWCLVGLPQLPAEPYLMLNSVKDGFYPA
                     SYEAMISMNALTFSTIMMSRLPSEEGSCLFDGFEDSGRVGMWTITQGETSPVTWNIQS
                     SQVCVAPVYLNECLPVPANEADISAEGTLLCQEEGIYDDGCIPSPGSLAGAGGGDFYA
                     WFGNPLTCSYSESGENCEFVGKRVSGALESPWINGIDAWTLYLMFDSAWEIESQTSSV
                     DVMYVEAQTSPQSETGLWTLVGTVNTMTPLDTAQVTRAAAAGGWTSGGDASGPTWNTY
                     KLDLKDFAGEFFRIRFRFDSMDGNANLYRGWQIDNISVSGIGCRSNEN"
     gene            complement(4125..4367)
                     /gene="rpmB"
                     /locus_tag="PKH54_05305"
     CDS             complement(4125..4367)
                     /gene="rpmB"
                     /locus_tag="PKH54_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOC99340.1"
                     /translation="MARCELTGKRRLRANKVSHANNRTPRWQHPNIQSKRIWVEELQG
                     NIRINLSTRALKSVTRLGLVAWARKNGLDLAKLVDR"
     gene            4581..>6730
                     /locus_tag="PKH54_05310"
     CDS             4581..>6730
                     /locus_tag="PKH54_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOC99341.1"
                     /translation="MFRCMACLAAVLMLASFAACGGGPNTTDVVSDVPGEVFVDTHQD
                     DVGEDGLDELPSDAGSDADAEMDGFDSMAEDLVDSDESDSGSDASPDSDIEVFESCTA
                     LTVDSDCVLTCAEAQACQVCSCNTDVPGGRCELVTAENGTECEDGENCTRDDYCENGT
                     CVPGRLACDCVSDSDCASREDGNLCNGTLFCDRGVFPHKCAVNPATVVPKCPTDNDTQ
                     CLAAGCVPETGVCEMLPANEGQTCTIVSTCILVATCQEGECAAFSVRDCNDNNPCTDD
                     QCVEQLGCVRLPNSVGCDDGNLCTTGDICMDGECGHTGTLDCDNGKFCDGPEGCEPSV
                     GCVPGTPPSCDDGVSCTLDRCLAFYDRCFNDWLPEAIEGPRGAPQCSDGKDNDCDGVS
                     DVSEPECLLGINRIEPDNGVLGGGDLIAISGNSLDLVQTVTIAGKPAEIVAQSNLSLT
                     VRTPPGDLPGRCDVEIRTSLVPFVSEDAFTYTTVESAPQGSCILVFPSQPVERDLGAS
                     IVVMQGQSNLPPSVEASVVRFSFGYGPRGSNPPITPGWKWTEATLVASGSVGGLVRTD
                     WSAFPVAVVGGYFDMAARMSVDGGVRWILCDLDGSANGYLPVQAPDLTVFGNAEPGDI
                     VINEMMWPGTTLDVNDEWIELRNLSDAPIRLDGWKITGVGYPSGQDFLFDGHDRVVRN
                     TSIEGGGYFLIAQFDAPDSVLDAQPDILAQASGSS"
BASE COUNT         1249 a         2009 c         1994 g         1478 t
ORIGIN      
        1 cttccttccc cggccatgac atcgagcagg ttcagggctg cgtcaacgat tgcctcggtt
       61 tccggcctgg gaacaagcac tgccggtgta acccggaagc tcaggccgaa gaactcccgg
      121 aagcccacga tatatgccac cggctccccc gcggaacgcc tgaccaggaa tttcctgagt
      181 gtatcgcgct ccagttccga aagtggcctg tccggtgcca ggtaaagatc cagccgcctg
      241 attccaagcg cgtgcgcggt aagtatctgg gcgtccagcc ttggactcgc ggatcccttc
      301 tttgcaagga atccgcttga gaggttcatc gcctgcgcca ccgtaagaat ttccggtccg
      361 ttcaacgtat caccccgggc tgacggccct gcctagaatg tatcatccgg ccttggcatc
      421 gggaagacga atccccctgc ctcctcgccg gccgcaagca gcctttcaag cgccatcctg
      481 agaccggccc tgtcattgat gtggggcaga ccgcgtcgga attcggtcgc gcccgccatg
      541 ccgcgcgtgt accttgcaag gtgcttccgg aaaaggcggg cggcgatacc ctcgaatccg
      601 tcaaggtaat ccagaaaaag attgaagtga tcccaaaccg tacgttccca ctcctgcctg
      661 ttaacgggag caggggcttc ctggccggcc aggcaggccc ttgtctgcga aaacagccag
      721 ggatttccaa tggcaccacg cgcaatcatg accgcgtcgc agccagtggt ttccatcatc
      781 cgcaatgcat ctgccgggga tctgatgtcc ccgttcccga tgacgggaat cctgcacgaa
      841 ctcttcatcc gggcaatcag gctccagtcc gcctttcctc gataggcctg gtttcttgtc
      901 ctggggtgaa tggttacggc ggccagatcc aggtcgttca ccatggccga aatttcatcc
      961 acgacgatgt tggactgggt cgggcccgat cgtactttca tggacaccgg tacatccacg
     1021 gcacctttga ccgcacgggc gatttcatga agcaggtcaa gatccgagag cagcgcggat
     1081 ccggcgccgg aattggtcac ccgctgtgac gggcatcccg cgttgatgtc gatgatgtcg
     1141 gcgccggcat tgaccgcaat ccgggcggcc tcgaccatga tgtctggctt cgctccctca
     1201 atctggacgg ccaggatcga ctctccgtct gccctgcgga gtcttcgcat ggcatcgctt
     1261 gccccaaaaa ccagggcgat tgcaggaatc atttcggtgt aacagagcgt ggcgccgtgc
     1321 cgcaggcaaa tcgttcgcca ggccgggtcg gagtacccgt caagcggagc gttgacaagc
     1381 ctgccttcga cgaccagccg tctgaattcc gaaaccggca tcgggcctgt cgtccttttc
     1441 gccagtgtgt ccagccgttt tctgactttc agtgggattc gcagggggat atgcggttcc
     1501 atcttctccc gatcaatttt cgttcgagcg gcaaccaatg ccgcttacgc tgatattgtc
     1561 gatctgccat ccgcggtaaa ggttggcgtt cccgtccatg gaatcgaaac ggaacctgat
     1621 cctgaagaat tcgccggcga agtccttcaa gtcgagcttg taggtgttcc aggtcggacc
     1681 ggaagcgtcg cccccggagg tccagccccc ggctgcggcg gccctggtga cctgcgcggt
     1741 atcgaggggc gtcatcgtgt tgaccgttcc aaccagtgtc cacaacccgg tttcggactg
     1801 cggtgacgtc tgggcctcga cgtacatgac atcgaccgat gaagtctggc tctcgatttc
     1861 ccaggcgctg tcgaacatca ggtacagggt ccacgcatcg atgccgttga tccatggcga
     1921 ctccagcgca ccggacacgc gttttccgac aaattcgcag ttttcgccag attccgagta
     1981 gctgcaggtg agcgggttgc cgaaccacgc gtagaaatcg cccccaccgg cgcccgcgag
     2041 ggagccgggt gacgggatgc atccgtcgtc gtaaattccc tcctcctggc acaggagggt
     2101 accttcggcc gaaatgtcgg cctcgttggc cggcaccggc aggcattcat tcaaatagac
     2161 aggggccacg cacacctggc ttgactggat gttccacgtc acaggcgatg tttcgccctg
     2221 ggttatcgtc cacatgccga cccttcccga gtcctcgaag ccgtcaaaaa gacaggagcc
     2281 ttcctcggac ggaagcctgc tcatcatgat ggtcgaaaac gtcagcgcgt tcatcgatat
     2341 cattgcctcg tagctggccg gatagaatcc gtcctttacc gagttcagca tcaggtatgg
     2401 ttccgcggga agctggggaa gtccgaccag gcaccagtac ccgttcgaat cagatgtcgt
     2461 cgtcgcgatt tcggttgacc ccgtcatgtc aaccagcgtg atgagagcag cgggaaccgg
     2521 actcccgctt gccggctccg tgctgccgct ggtcgtgaca acgtagtcgt cgtactcgag
     2581 aacgcggccg cacatcgaga gctgtgcctg ggtcatgccg ttcagaagaa gaatcatctc
     2641 gatcggaatt accgttccag gttccagaaa cagaacatcg gttgtcatcg cctggtagcc
     2701 ttcggcactg acctgaatgc accacagccc gggttcaatc ggaatctcag actttccttc
     2761 gtcgtcggtc gtcacggtcc acgccgcgct caactcggtc tctggcgggc atgaatcctt
     2821 gtagaagctg accgaagctc ccgacagggc ctggtatcct tccccggtcg cagaggactt
     2881 gaatggaggc ggtggcaggc tcgccccgaa caccttcatc atgatcgtcg atgtccggtc
     2941 gcagatgaag tcttcgtccg tcattccatc gcagtcgttg tcgattgtgt cgcagatctc
     3001 ctgggccgga gtcatcgcat cgcagtcggc gccaccggtt tgaatgtcac aaacagtctc
     3061 gcccgtgcag gttccgaatt cgttgacgat ttcgcattcg attccgacca gatcctcgtc
     3121 ggtcgaaccg ttgcagttgt cgtcgatgcc gttgcagatt tcggccgccg gcgtctgtcc
     3181 gatgcaggta acgcccttga attcattgca ggccgtcgtt cccgcgcatt tgccgtattc
     3241 gttgcggttg aagcaatcct gtccgaccag tccttcgtcg gtctcgccgt cgcagttgtt
     3301 gtcgatcgaa tcgcagctgc tttcgttcgg ttcccagccg gttatgcccg agtactcaca
     3361 gacccattca cccctgaggc agacgcggaa gatgacttcc tgcctgcccg cgcaaatccc
     3421 cttgtggctg catgccggct gactgggagg ttgcagtccg atgtcggcct tgccgtcgca
     3481 gtcgttgtcc agtccgtcgc atgtggcttc ggtttcctcg aatgacggaa cctgcgagta
     3541 gtcgcatgcg gccacgccct gggcgcatgt ggcggtcact ttgtcgaacg ccagtccgca
     3601 cacgcccgcc ctggagcaat tgagttcggc aactttcgcg gggttctcgg aaaactcgtt
     3661 gttctgcgcg tcgaattcat ccggcaggca gtccccgtcg ggatcggccg tcggaaggcg
     3721 ggactcgagg gcgtcgatga ttccgtcgcc gtcgccgtcg agaggcgaat tgacgtcatt
     3781 gccgacttcg gtgccgtcgt tgattccgtc gccgtcagta tccgccagtc cgtagctggt
     3841 tccggtgata ttgcgttcga ttccgttctt gatcccgtcg agatcgaagt cgccgtccgg
     3901 attaaggcac tgcgaatcat agcagagccc gctttcgcaa tcggcttcgc tttcgcagaa
     3961 ttccccgatc tttcttggat ccgagccttc acatgatgcg agaagcatgc agaaaaagac
     4021 cgacggaagc acgattgaag tgaatcgttt cattatgaac ctccggttcg ggcagaagga
     4081 tataagttgt ccggaggtag tataagcgaa cctggcgcca atggctatct gtcaaccagc
     4141 ttggcgagat ccaggccgtt cttccgggcc caggcgacca ggccaagcct ggtgaccgat
     4201 ttcagggcgc gggtcgaaag gttgatacgg atattgccct gcagttcctc cacccagatc
     4261 ctcttggact ggatgttcgg gtgctgccac ctgggcgtcc tgttgttagc gtgcgaaacc
     4321 ttgtttgcac gaagtcttct ctttccggtc agttcgcatc ttgccatttg gtcgtctcca
     4381 aaacacaaag cgcgccgaag gttacacggt cagtgccgcc aagtcaatag gcgtttgatc
     4441 atttccgggc gggattttgt ctcgcgtccc cccgaaaagg gtcctttgaa gacatgccgt
     4501 tgccatcgga tgcattattc atggataatc tacccggctt cgggcaacgc agaaaccggt
     4561 cgcccgatgg aggaatccca atgtttcgct gcatggcgtg tcttgccgcc gtattgatgc
     4621 tggcctcctt tgccgcctgc ggtggcgggc cgaacaccac ggacgtcgtg tccgacgtgc
     4681 ctggtgaggt gtttgtcgat acccaccagg acgatgtggg cgaagacggg ctggacgaac
     4741 ttccttcgga tgccggcagc gatgcggatg ccgaaatgga cggcttcgac tcaatggccg
     4801 aggatcttgt ggattcggac gagagtgatt ccggttctga cgcgtctccg gattccgaca
     4861 tcgaggtctt cgagtcctgc accgctctga cggtcgattc cgattgcgtt cttacgtgtg
     4921 ccgaagcgca ggcctgccag gtttgcagtt gcaacaccga tgtccccggc ggccgttgcg
     4981 aactggtgac cgcggagaat ggaaccgaat gcgaggacgg cgagaactgc acgcgcgatg
     5041 attactgtga aaatggaacc tgcgttcccg gtcgcctcgc gtgcgactgt gtttccgatt
     5101 ccgactgtgc ttcccgtgaa gacgggaatc tttgcaacgg cacgcttttt tgtgatcgtg
     5161 gcgttttccc gcacaagtgc gcggtgaatc cggcaaccgt ggtgccgaag tgtccgactg
     5221 acaatgacac tcaatgtctt gccgccgggt gcgtgcccga aaccggcgtc tgcgagatgc
     5281 tgccggcaaa tgaaggccag acctgcacca tcgtcagtac ctgcattctt gtcgcgactt
     5341 gccaggaagg cgaatgcgcg gcattttccg tgcgcgactg caacgacaac aacccgtgca
     5401 cggacgacca gtgtgtcgag cagctcgggt gcgtcaggct tccaaacagt gtcggatgcg
     5461 atgacggcaa cctttgcacg acgggcgaca tctgcatgga cggggagtgc ggtcatacag
     5521 gcacgcttga ctgcgacaac ggcaagttct gtgacggtcc ggaaggctgc gagccttccg
     5581 tcggatgtgt tcccggcacc ccaccgagct gtgatgatgg tgtttcatgc acccttgatc
     5641 gctgcctggc gttttatgac agatgtttca acgactggct gccggaagcc atcgagggac
     5701 caaggggcgc tccgcagtgt tcggacggaa aggataatga ctgtgacggg gtgtcggacg
     5761 tttccgagcc ggaatgcctg ctcggcatca acaggattga accggataac ggcgtcctcg
     5821 gcggtgggga tctgatcgcc atcagcggaa attcgcttga ccttgtccag accgtgacca
     5881 ttgccggcaa gccagccgag attgtcgccc agtccaacct ttcgcttacg gtgcggactc
     5941 ctccggggga tctgcctggc cggtgcgacg tggaaatacg aaccagcctg gttccgttcg
     6001 tttccgagga cgccttcaca tacacgaccg tcgagtccgc gccccagggg tcgtgcatcc
     6061 tggttttccc gtcccagccg gtcgagcgcg atcttggcgc atccattgtg gtcatgcagg
     6121 gccagagcaa tctgccgcca tcggtcgagg cttccgtcgt gcgtttcagc ttcggatacg
     6181 ggcccagagg ctcaaatccg ccgattacgc ccgggtggaa gtggaccgaa gcgacgctgg
     6241 ttgcaagtgg aagtgtcggc gggctggttc ggactgactg gagcgcattt ccggtggccg
     6301 ttgtcggcgg atacttcgac atggcggcca ggatgtcggt cgatggcggg gttcggtgga
     6361 ttctgtgtga ccttgacgga tccgccaacg gttacctgcc ggttcaggcc cccgacctta
     6421 ccgtttttgg aaatgccgag cccggcgaca tcgtgatcaa cgaaatgatg tggcctggaa
     6481 caacgctgga cgtcaacgat gaatggatcg aactcaggaa tctttccgat gccccgataa
     6541 ggcttgacgg ctggaagata accggcgtcg gttatccgtc gggccaggat ttccttttcg
     6601 acggtcatga cagggtggtc aggaacacga gcatcgaggg cggcggatat ttccttatcg
     6661 cacaattcga tgccccggat tcggttcttg acgcccagcc cgatatcctg gcgcaggcca
     6721 gcggttcctc
//
LOCUS       DAOPPA010000127         6717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12520_length_6717_cov_3.978235, whole genome shotgun sequence.
ACCESSION   DAOPPA010000127 DAOPPA010000000
VERSION     DAOPPA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12520_length_6717_cov_3.978235"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..4354)
                     /locus_tag="PKH54_05315"
     CDS             complement(209..4354)
                     /locus_tag="PKH54_05315"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99342.1"
                     /translation="MQRRHLGFMIRAALAALLMLGGCTGVVEQGDLDASVTPDLPIID
                     IAVDSHYFDQHSPDGGDSGLDGSSDDIPDAGVDSIDDIFVDIAEGEPGWPCDQPGDCN
                     SGFCLDTPDGLKCSSLCGGSSSCPRGYTCTTFSTGSDAVSACVHNMPRVCMPCMDDDE
                     CKSLQTGHSIACVGDDGGRFCAGGCTTEMECPAGYSCTEATVGSGSKALVCRPADGEC
                     GCSDRAILESAATECVLKNEIGTCVGQIVCTEGGLQPCSVQEPATETCNLKDDDCNGT
                     TDDVAVRACLVQNSFGSCPGTTLCQSGTETCQGQRPEQEQCNEIDDNCNGTTDEENAG
                     GCRSYFEDIDSDTFGNSQKGKCLCGPSGKYTATRGGDCNDQNVEINPDRTESCNGIDD
                     NCNGSTDEENATGCRSYYYDNDADSYGTSAAPKCLCVATRPYSALRTGDCNDSNTNVN
                     PGMVELCNTIDDNCDGATDPVDAGGCQSFYYDFDSDGYGSATVAPKCQCGPDYLTRFK
                     VQVAGDCNDSVVTISPAATEKCDGIDNNCNGTTDENGAINCVNRWMDNDGDGYGTGTS
                     VCSCQAAAPYTAERAGDCNDSDFFINPGISETCDGIDNNCDSKTDDENAGGCDAWFFD
                     GDRDGYGDPTKSRCLCGPSVSTKYDSDNGLDCNDAVTSISPAGTESCGNGKDDDCDGK
                     TDDENAHGCDLFYYDSDGDGFGILTIFKCLCAADGLYRTLTPGDCDDNAINSFPGGQE
                     TCSAGDEDCDGTFNEQDATGCVTYFYDGDADDYGDPLIAGKCLCAASPSTGFRALVNT
                     DCNDGDSSINPGKTEVCEPDGTPSVDNNCNSFMNEENAIGCKSYYYDFDRDGHGLKNS
                     TPKCYCTGGNVGLRYTSLVADDCNDNDNTVSGGLPELCDGKDNDCDSSTDENGASDCT
                     TYYLDTDRDGYGVLNNSECRCAPDPGRWYDATAPGDCNDGNNLIYPGQSVCGVDGSCD
                     GTLLDPLEECDDGNSTKWDGCTDCRASEYRINQQTSGDQSWPSSALKSDGGWAVVYSS
                     VRNTTGEDIAVRILSSTGTTQGSEVFANTYTANTQTYPDIAAMASDIVLVWQSDGQDG
                     SGYGIVGRIMNADGTAKYPEFQVNGTSGNAQARPAVAVGSDDRIVTVWQSNLQDGSLE
                     GVYGRIHVAPGAAANSEVLIPQTTALSQTNPDIAIYKAGHRIVVTWTGEVQIDASTTD
                     TRIFARIFDEDLVPVTNEFQVTNSGTTVVGQNNSAVAVTGSVGFVVAFEDYSIDGSER
                     GIRYRAFDWNGNALGAPAIANTFTTGLQAAPSVIGIDDTRFIIAWWSEFGDTDGSAAC
                     IARRFVRGGTNGSETILHGVTANYQFYPALAVSSNSLWYAAWMSSQQDGDGRGIYGRT
                     FSF"
     gene            complement(4494..4994)
                     /locus_tag="PKH54_05320"
     CDS             complement(4494..4994)
                     /locus_tag="PKH54_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOC99343.1"
                     /translation="MTENPSAEDRDAGQTTAASSRQGKRRSRTIAARRLSKVDFSDGL
                     EIDESLEALRPRTRADCAEGPRPCPWVACKYHLYLDVSTRTGSIKLNFPDLEPWELQD
                     SCVLDVADKGPVTLEDVGRIMNLTRERIRQLEQAASTRIKDTRLAREYEKFTLERPRP
                     EETPST"
     gene            complement(5124..6236)
                     /locus_tag="PKH54_05325"
     CDS             complement(5124..6236)
                     /locus_tag="PKH54_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC99344.1"
                     /translation="MKDIINVGLIGSGMISQVHFNALQTVPGAKVVAVADLNQERARD
                     FAASRGITKVFRTHAELLADQEIDAVVVAIPNYLHAPVSIDALEAGKHVICEKPLALT
                     LEDAERMIATARRKGLVLAHAEELCFIPKFVRAQELAASGGIGKPYLLRQCEKHDGPY
                     SPWFWKPAEAGGGIVMDMGCHAIECIRFFLGKKKVKSVMAHMGTYLHGATTQEEDHCI
                     IIMEFEDGTVGQAEASWALRGGMDSTFEIFGTDGVVYADLLKGSALRAFSREGFPGSD
                     GPSNGWTFHDYEWLWNNGYPQEDAHFVECMLEGKTPVESGEDGLAVLEIILAAYHSAG
                     IGRKVNLPFRPKGIPYPVDLWLNPRPELGAGPVSEL"
     gene            complement(6253..>6717)
                     /locus_tag="PKH54_05330"
     CDS             complement(6253..>6717)
                     /locus_tag="PKH54_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99345.1"
                     /translation="EVTMSRQKMAAEFRDLLHSSVNGLRVLDPDPRQTRLHAYQTVMK
                     YDPAQFGGVHRDRFLEALEAEGVGFHGDFYTALHEHELMDAKSDQYPMLRERYGDGIH
                     SVKGNFPVSVKAGYHEAVWMHYSYLSGTRKDLQDVVEAILKVQSNVGELAGR"
BASE COUNT         1200 a         2008 c         1984 g         1525 t
ORIGIN      
        1 tggttaagat tcttgcctgg tacgacaacg agtggggtta ctcgaaccgc tgcgttgacc
       61 tgatcgaagc cgccgtcgcc ctggactgat ttcgcatctt ccaagcccca ttctcaaggc
      121 cagttcgcca atcgactgtc gcgaatctgc gacctgttcg gcgcattcag acggtgtcca
      181 gtaaaacaga caggcagttg tcgatagttc agaagctgaa tgtgcggccg tatatgcccc
      241 gtccgtcgcc atcctgctgg gacgacatcc aggcagcgta ccacagcgag ttgctgctga
      301 ccgccagcgc cggatagaac tggtagttgg ccgttactcc gtggagtatg gtttcgcttc
      361 cattcgttcc gccgcgcacg aatcgccttg cgatgcaggc agccgatccg tccgtgtccc
      421 cgaattccga ccaccatgca atgatgaagc gggtgtcgtc gatgccgatg acggaaggag
      481 ccgcctgcag gccggtcgtg aaggtattgg cgattgccgg cgccccgagg gcgttgccgt
      541 tccagtcaaa ggcgcgatac ctgattccgc gttcgctgcc gtcgatggag tagtcctcga
      601 aggcgaccac gaagccgacg gatccggtca ccgccactgc gctgttgttc tggccaacaa
      661 cagtggtacc ggaattcgtg acctggaatt cgttggtaac cggaacaaga tcttcatcga
      721 aaatcctggc aaagatcctt gtatcggtgg tcgaggcatc aatttgaacc tcaccggtcc
      781 aggtaacgac gatgcggtgg ccggccttgt aaatggcaat gtccgggttt gtctggctga
      841 gagcggtcgt ctggggaatc aggacttcgg aattcgccgc cgcgccgggg gccacatgga
      901 tgcgaccgta aacgccctca aggctgccgt cctgaaggtt ggactgccag acggtcacaa
      961 tccggtcatc ggaaccgacc gcaacggccg gtcgcgcctg ggcgttgccg gatgtgccgt
     1021 tcacctggaa ttccgggtat tttgctgttc cgtcggcatt cattatcctt ccgacgatcc
     1081 cgtaacctga accgtcctga ccgtccgact gccatacaag cacgatgtcg ctggccatgg
     1141 cggcaatatc cggataagtc tgggtgttgg ccgtatatgt gtttgcaaag acctcggatc
     1201 cctgggtagt tcccgttgac gaaagaattc tgaccgcgat gtcctcgccg gtggtgttcc
     1261 ttacagacga atatacgacc gcccagccac cgtccgactt cagtgccgac gaaggccagc
     1321 tttggtcgcc gcttgtctgc tggttaatcc tgtattccga agcccggcag tccgtgcagc
     1381 cgtcccactt cgtggagtta ccgtcgtcgc attcctccag cgggtcaagc agggttccgt
     1441 cacagcttcc gtcaacgccg caaacggatt gcccgggata gatcaggttg ttgccgtcgt
     1501 tgcagtcacc cggggccgtc gcatcgtacc atcgcccggg atccggcgcg cagcggcatt
     1561 cgctgttgtt caggaccccg tatccatcgc gatcggtgtc gaggtaatag gtcgtgcagt
     1621 cgcttgcgcc gttttcgtcc gttgacgagt cgcaatcgtt atccttgccg tcgcacagtt
     1681 caggcagccc cccggatacg gtgttgtcgt tgtcgttgca gtcgtcggca acaaggctcg
     1741 tgtaccttaa gccaacgttt ccgccggtgc agtagcactt cggggtcgag ttcttcaggc
     1801 cgtgtccatc gcggtcaaag tcgtagtaat acgacttgca gccgatggca ttttcctcgt
     1861 tcatgaacga attgcagtta ttatcgacgg aaggcgtgcc gtcaggctcg cagacttcag
     1921 tctttcccgg gttgatcgaa ctgtccccat cgttgcagtc ggtgttcacc agtgccctga
     1981 agccggtcga tggacttgcg gcgcaaaggc atttgccggc aataagcgga tcgccgtagt
     2041 cgtcggcgtc accgtcatag aaatacgtga cgcagccggt cgcgtcctgc tcgttgaagg
     2101 tgccgtcgca atcctcgtcg ccggcgctgc acgtttcctg gccgcccggg aacgagttga
     2161 tcgcgttgtc gtcgcaatcg cccggtgtca gggttcggta gagcccgtcg gctgcgcaca
     2221 ggcacttgaa gatcgtcagg atgccaaaac cgtccccatc ggaatcgtaa tagaacaggt
     2281 cgcacccgtg agcgttctca tcgtcggtct tgccgtcgca gtcgtcgtcc tttccgtttc
     2341 cgcatgattc cgtcccggcc gggcttatcg atgtcacggc gtcgttgcaa tcaagtccgt
     2401 tatccgagtc gtatttagtc gacacggatg gcccgcagag gcaacgggat ttcgtcggat
     2461 ccccgtatcc gtcgcggtcg ccgtcgaaga accatgcgtc gcatccgccg gcgttctcgt
     2521 cgtctgtctt tgagtcgcag ttgttgtcaa ttccgtcgca agtctcggaa attccaggat
     2581 taatgaagaa gtccgaatca ttacaatcgc ctgcacgctc ggccgtgtaa ggggccgccg
     2641 cctggcaact gcagacggac gtgccggttc catacccgtc cccgtcgttg tccatccatc
     2701 ggttgacaca gttgatggcc ccgttttcgt cggtcgtgcc attgcagttg ttgtcaattc
     2761 cgtcgcattt ctcggtggct gccgggctga tcgtcaccac gctgtcgttg cagtcgccgg
     2821 cgacctgaac cttgaacctt gtaaggtagt ccgggccgca ctggcacttt ggcgcgactg
     2881 tggccgaacc gtagccgtcc gagtcgaagt cgtagtagaa gctctggcag ccacccgcat
     2941 caacaggatc ggttgccccg tcgcagttgt cgtcgattgt gttgcacagt tcgaccattc
     3001 caggattaac gtttgtgttg gagtcgttgc agtccccggt tctgagggcc gagtaggggc
     3061 gcgtggcaac gcacaggcac ttcggggccg cgctggttcc ataggagtcc gcatcgttgt
     3121 cgtaataata gctgcggcag ccggtggcgt tttcctcgtc ggtcgaaccg ttgcagttgt
     3181 catcgatgcc gttgcaggat tccgtcctgt cggggttgat ttcgacattc tggtcgttgc
     3241 agtcgccacc cctggtggcc gtgtacttgc cagaagggcc gcaaaggcac ttgcccttct
     3301 gggaattccc gaacgtgtcg gaatcgatgt cttcgaagta actcctgcat ccgccggcgt
     3361 tctcttcgtc ggtcgtgccg ttgcagttgt cgtctatttc attgcattgt tcctgttccg
     3421 gcctctggcc ctggcacgtc tcggttccgg actggcatag cgtggttccc gggcaggagc
     3481 cgaaggagtt ctgcaccagg catgcccgaa ccgcaacatc atcggtcgtt ccgttgcagt
     3541 cgtcgtcctt caagttgcat gtttcggtcg ccggttcctg aaccgaacag ggctgaagtc
     3601 cgccttccgt gcagacgatc tgtccaacgc acgtgccgat ctcgttcttc agcacgcatt
     3661 ccgtggcggc ggattcaagt attgcccggt cggaacatcc gcattcgccg tcagccgggc
     3721 ggcataccag ggcctttgaa ccggagccca cggtagcctc ggtgcacgag tagcccgccg
     3781 gacactccat ttcggtggtg cagccgccgg cgcagaacct gccgccgtca tcgccgacgc
     3841 acgcgatgct gtggccggtc tgcaggctct tgcactcatc gtcgtccatg cagggcatgc
     3901 agacgcgggg catgttgtga acacaggccg agactgcatc cgagccggtt gagaaagtcg
     3961 tgcacgtgta tccccgcgga catgacgacg aaccaccgca gagagacgag cacttcagtc
     4021 cgtcaggggt gtcaaggcag aacccgctgt tgcagtctcc cggttgatcg catggccatc
     4081 ccggctcgcc ttccgcgata tccacgaaaa tgtcgtcgat cgaatcgacg ccggcatccg
     4141 ggatgtcgtc ggatgacccg tcaagtccgg agtcgccgcc atcgggagag tgctggtcaa
     4201 agtagtgtga atcgacagca atatcgataa tgggcaaatc gggcgtaacc gacgcatcga
     4261 ggtcgccctg ttcgacgacg ccggtgcacc cgcccagcat taaaagggcc gccagggctg
     4321 cccggatcat gaatcccaaa tggcgacgct gcatcgcgcg acctccatgg acggttttcg
     4381 ccccaaaatc ggcaggaaac ggttcaccgt attatacaga cagaaacggc ttcaggctac
     4441 atggcggtga atgtcggagg tgcggatggt ggacagtcat gcacggatac ggttcaggtt
     4501 gaaggggttt cttccggtcg aggtctttcg agcgtgaatt tctcgtattc acgggcaagt
     4561 ctggtgtcct tgattctggt ggaagcagcc tgttcgagct gtcggattcg ttctctggtc
     4621 aggttcatga tcctcccgac atcttcaagg gttaccgggc ctttgtcggc gacatccaga
     4681 acgcaggagt cctggagttc ccatggttcc agatccggga agttcagttt gatcgacccg
     4741 gttctggtgc tgacgtccag atacagatga tatttgcagg cgacccaggg gcatggcctg
     4801 gggccctcgg cgcaatcggc cctggtcctg ggacgcaggg cttccaggct ctcgtcgatt
     4861 tccaggccgt ccgagaaatc gaccttgctg agccgtctgg ccgcaatggt ccgcgatctg
     4921 cgcttgccct gacggctgga cgcggcagtt gtctggccgg cgtcacggtc ttcggccgaa
     4981 ggattttcag tcatcggcat ctcgctgttg tctggttttt cggaagccat tccacgcaaa
     5041 ccatatctgc atcggcttgc gacattagca catcgtttgg tgtgtgaaaa gcacctattg
     5101 aagtgttgct ggaaggattc tgttcagagt tcgctgacag ggcccgcgcc cagttccggt
     5161 cgaggattca gccacaggtc aacaggatag ggaatgcctt tcgggcggaa cggaaggttg
     5221 accttgcgtc cgattccggc gctgtggtag gcagccagga tgatttccag gaccgccagt
     5281 ccgtcttcgc cggactcgac aggagtcttt ccttcaagca tgcactccac gaaatgtgcg
     5341 tcttcctgcg gatagccgtt gttccagagc cattcgtagt cgtggaacgt ccagccgttg
     5401 gaaggtccgt cgcttcccgg gaagccttcc ctggaaaatg cccgcagggc gctgcccttc
     5461 agcaggtcgg catataccac gccgtcggtt ccgaagatct cgaacgtgct gtccattccg
     5521 cctctgaggg cccaggaagc ctccgcctgg ccgacggtgc cgtcctcgaa ctccatgatg
     5581 atgatgcagt gatcctcttc ctgcgtcgtg gctccgtgca ggtatgttcc catgtgcgcc
     5641 atcacggact tgaccttttt cttgcccagg aagaaccgga tgcattcgat tgcatggcag
     5701 cccatgtcca tgacgatgcc gccgccggcc tcggccggct tccagaacca tggggaataa
     5761 ggtccgtcgt gcttctcgca ctggcgcaga aggtacggct tgccgattcc accggatgcc
     5821 gccagctcct gtgccctgac gaacttcgga atgaaacaca gttcttccgc gtgcgccagg
     5881 acgaggccct tgcgcctggc cgtggcgatc atcctttcgg cgtcttccag cgtcagcgcc
     5941 agcggctttt cacagatgac gtgtttcccg gcctccagtg catcgattga caccggcgca
     6001 tgcaggtagt tcggaatcgc gacgacgacg gcatcgattt cctgatccgc cagcagctcg
     6061 gcgtgagtcc ggaagacctt cgtgatgcct cggctggcag cgaaatccct ggccctttcc
     6121 tggttcaggt cggcaaccgc gacgactttt gcccctggca ccgtctgcag cgcgttgaaa
     6181 tgaacctggg aaatcatccc ggatcctatg aggccgacat taatgatgtc cttcatgatg
     6241 tcacaccccc gatcacctgc cggccagttc gccgacgttg ctctgaacct tcaggatcgc
     6301 ttcgacgacg tcctgaagat cctttctggt tccggataga tagctgtaat gcatccagac
     6361 ggcctcgtgg tagcccgcct tgacggaaac cgggaagttc cccttcacgg agtggatgcc
     6421 gtcgccgtat cgttcgcgca gcatcgggta ctgatctgac ttggcgtcca tcagttcgtg
     6481 ctcgtgaagc gccgtgtaga agtccccgtg gaatccaacc ccttcggcct cgagagcctc
     6541 gaggaaacgg tcgcggtgaa ccccgccgaa ctgggcaggg tcgtacttca ttacggtctg
     6601 gtaggcgtga agcctggtct gccgcggatc cggatcaagc acgcgcaggc cattcacgct
     6661 ggagtgaagc agatcgcgga attcggccgc catcttctgg cgtgacatgg tgacctc
//
LOCUS       DAOPPA010000128         6635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12726_length_6635_cov_3.586322, whole genome shotgun sequence.
ACCESSION   DAOPPA010000128 DAOPPA010000000
VERSION     DAOPPA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12726_length_6635_cov_3.586322"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..1325)
                     /locus_tag="PKH54_05335"
     CDS             complement(318..1325)
                     /locus_tag="PKH54_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOC99346.1"
                     /translation="MNKTLLLLMPAATAIFLACDNGVHYCDTDLEYQFHQLLPIPDEG
                     WMADPHVIRVGCTWYLYATHSQVDLQVWYSDDLTNWTQGPTIWAPTQDWQTEGNLCGI
                     WAPHVEPAGDKFFLYYSANCRIGVAVANDPLGPFVDVYDHPLVGNGYGGVGDGQLVND
                     LFLDYDDLAIDAFVLQTAGGQRILFHNAFTPASTVHAQEMPDFVSVSGKPLVMIEPDL
                     NSWEGFIREGTWVLEHDGVFHLMYSGNDCFTAEYAIGVATATNPMGPYVRDPRNPILQ
                     KNPESEFWGPGHHSVTYDATGNPLMFYHTKTTPDQGEDRLVRYGPLWFDELGRVNVEQ
                     P"
     gene            1436..3946
                     /locus_tag="PKH54_05340"
     CDS             1436..3946
                     /locus_tag="PKH54_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOC99347.1"
                     /translation="MAAINESRPGLKEPYNLSPRIGWLRDYYFKGTDRKWNNEFTAWT
                     TGTPWDVCFQEMTYYIVPEVYFLMQALRGSYRQGAREVPLDADFWNWSLPERRAWFVR
                     KVMVDMVPQEILPGDLVCGARFNIQTSLCLNRDEAAEYDRLLIGPNGARATMKRFHDH
                     GYGNSGATSGHLVPGYERMLKIGWRGIHQEIEGHYQALSPEDKAGPRGCQLRAMMTAA
                     TMPRDVGVKYAARCRELAAVEPDEGRRAELAAMASNLDHVPWEPPTTFWQALQALWLG
                     HMLVMSDENYPGPGVSFGRFDQYMLPYWQRSIAEGMDREFGKELLKCFWVHCNTAYDA
                     QIRSGNQGITAGFGQLITLSGMGADGRDMTNDFTYAVLEVIDEMSPILEPKPNVRLHR
                     GTPPELMDKIVQMISDSQGAPFLLNFDERSMAGMLRQAELAGMKDRINLDNVHEYAPV
                     GCLENTMVGNDRSGTVDNNLNLVKAVELAINGGRDLLPYVDQITGVAEPVTQDGPDTG
                     DATTFSDFEEFWAAYERQTAYIIDRCASTYEASESVRARFFQTPYLSCLVAGCAEKGR
                     DINQGAAEINLTTLEAVTFATTVDSLLAVKYLVFDRKICTMGKLIDALRANWAGHEVL
                     QAQARNKAPKYGRDDDEADAMAVRVMNLWCDGAWKHVTKSTGRRFRPGMLSWNYWAGD
                     GYVVVATPDGRPRGQFLSNAICPSNGADINGPTSNSNSVGKALGGKSEDQVGNYRGYV
                     NLLPNGASHTITFHPAVIQDPEHCEKFKSFLHGYMENGGTALQINILDPDMLRMAQKH
                     PQDYRHLLVRVTGYNAYFVSIGRELQNEVIARMSHQRF"
     gene            3971..4972
                     /locus_tag="PKH54_05345"
     CDS             3971..4972
                     /locus_tag="PKH54_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOC99348.1"
                     /translation="MGGLEASASIVSDEIMKDGLVLEIQRMSTEDGPGLRTTVFLKGC
                     PLRCSWCHNPESISPQPELQWIPVRCIECGTCVGFCSRGALSRGVEGVVIDRGACAGC
                     GACADACPTNALEIWGRRMTAQALVREVAKDAAYFSRSGGGVTLSGGEATAQSEFCMD
                     VLRGLRASGLNTALDTCGLCRPETLQAMLPLCDVVLYDVKMIDSNLHRQFCGASNEVI
                     LENLRLVASAVVGSAGATSLWVRTPLIPGATATIDNMKAVGGFLATLPPGSVSRWDLC
                     AFNHLGRDKYVRLGIDWQYRDMPLMTAAELDAMKAAAQSSGVDPAIVRTSGATRLQE"
     gene            4986..5822
                     /locus_tag="PKH54_05350"
     CDS             4986..5822
                     /locus_tag="PKH54_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99349.1"
                     /translation="MNTLSEGAMKALNAGTKLGLIATVDPAGLPHITLMTSLEAKDPN
                     HLMFGQFSSGKSKEHLRSNPLMAFVAVTLDKQTLSGHGRYTHSVREGADYEHYNMQPM
                     FRYNTYFGINTVHYLDLLDATDVAPLPMSRIVPAAIITRLVKGVLADGSRPQPLTSMA
                     RDLFNRLDSLKFMAYVDDSGMPQMFPVIQCQAADAGRLAFSTIAGGPQLRSVPVGATV
                     AVFAMNMMMESILVRGTFNGLSRRAVFQSGTVDIDWVYNSMPPCHGQIWPEVPLEPVE
                     SF"
     gene            complement(5879..>6635)
                     /locus_tag="PKH54_05355"
     CDS             complement(5879..>6635)
                     /locus_tag="PKH54_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99350.1"
                     /translation="RFDLPSVTDLFSIKEAPDGTIHAVYSKTSIPCDPCDMGLYHGTL
                     PMDGQWSEDLIQESKWGDPNDEYATDAALAIDSNGHPLIAATYFVRAITGSIKSAQLR
                     LYGSGQSGWCNETVVTEVDGYAGSDGTKMTGISPSIFMDTSDRPHVVFMDKAQWHDGN
                     NWANAIDGQIRYAVRNGRKWTVRTLLTQDGQTKSPSPLIGALPPSLAISPDGTSIHAA
                     TATFEWDTDSIYLQQATDISFKATAIQAESTLP"
BASE COUNT         1319 a         1896 c         2067 g         1353 t
ORIGIN      
        1 atcggatcaa ggaagatgcg ctgggcgtca attatcgcca ggccagggcg gcgcacagcg
       61 aattcgatgt gccggccccg aagatgtccg tcatgctcgc agacctgggc accttcacgc
      121 agttcatcat gacaatcgac ggcacctttg gcttgatctt tcgaattggt tgaaaaaatt
      181 gacatgacag accatccgtt tcaagaaaac gcgtaaaaac gcgcctttgg ctggatggcc
      241 cgccaggggg cctgacattg ccgaaagcgt tatgtcagga ctcttttacc agacagcggg
      301 atgcgaatca agggtggtca cggctgctcg acgttgacgc gccccagttc gtcgaaccac
      361 agcggaccgt acctgacaag gcggtcttca ccctgatcgg gtgttgtctt ggtgtgatag
      421 aacatcaacg gatttcccgt cgcgtcataa gtcacgctgt ggtgccccgg gccccagaat
      481 tcgctttccg gattcttttg caggatgggg ttcctgggat cgcggacata cggtcccatc
      541 gggttggtgg cggttgccac gccgatcgcg tattcggcgg tgaagcagtc gttgcccgaa
      601 tacatcaggt ggaacacacc atcatgctcc agtacccagg ttccttcccg gatgaagcct
      661 tcccaggaat tcaggtcagg ttcaatcatg accagcggct tgccggaaac cgacacgaaa
      721 tcgggcattt cctgggcatg gacggttgaa gccggcgtga aggcgttatg gaaaaggatt
      781 ctctggccgc cggccgtctg cagaacaaag gcgtcgatcg ccaggtcgtc gtagtcaagg
      841 aagaggtcat tgacaagctg gccgtcgccg accccgccat atccgttgcc gacaagggga
      901 tggtcatata catcgacgaa tggccccagc gggtcgttgg cgaccgccac gccaatgcgg
      961 cagttggccg agtagtacag gaagaatttg tctcccgccg gctccacgtg cggtgcccag
     1021 atgccgcaaa ggtttccttc ggtctgccag tcctgcgtcg gcgcccagat cgtggggccc
     1081 tgggtccagt ttgtcaggtc atccgagtac cagacctgca aatccacctg ggaatgggtc
     1141 gcgtaaaggt accatgtgca gccgacgcga atgacgtgcg ggtccgccat ccagccttcg
     1201 tccggtattg gaagcaactg atggaattga tattccagat cggtgtcgca gtaatggaca
     1261 ccattatcgc atgccaggaa gatggcagtc gcggcaggca tcagcagcag caaggtcttg
     1321 ttcacttgag tccccccttt atccttcggt acggccctgg ccggccgaat cagcatagca
     1381 cactgcgctt ccgggtatta caatcgtgcg gtcacggtca atcccagggg gtttcatggc
     1441 agccatcaac gaatcaaggc cgggcttgaa ggagccgtac aacctgtcac caaggatcgg
     1501 gtggctgcgc gattattact tcaagggcac ggatcgcaag tggaacaacg agttcacggc
     1561 gtggacgacc gggacgccat gggacgtctg ttttcaggaa atgacgtact acatcgttcc
     1621 cgaggtctat ttcctgatgc aggccttgcg tggctcgtac cgccagggcg cgcgggaagt
     1681 tccccttgat gccgacttct ggaactggag ccttcccgaa aggcgggcgt ggttcgtccg
     1741 caaggtgatg gttgacatgg ttccgcagga gatccttcca ggggaccttg tctgcggggc
     1801 gcggttcaac atacagacgt cgctgtgcct gaaccgcgac gaggcggccg aatacgacag
     1861 gctgctgatt ggtccgaacg gcgcccgcgc caccatgaag cgctttcacg accacgggta
     1921 tggaaacagc ggcgcgacca gcggccacct ggttcccgga tatgaaagga tgctgaagat
     1981 cggctggcgt ggaattcatc aggagatcga agggcactac caggcgcttt caccggaaga
     2041 caaggccggc ccccgggggt gccagcttcg agcgatgatg accgccgcca cgatgccgcg
     2101 tgacgtcggc gtgaagtatg cggcgcgttg ccgtgaactg gccgccgtcg aaccggatga
     2161 aggccggcgc gcggaacttg ctgccatggc gtccaacctc gaccacgtcc cctgggaacc
     2221 gcccacgacg ttctggcagg cgctgcaggc cttgtggctt gggcacatgc tggtcatgag
     2281 cgatgaaaat tatcccggcc ccggcgtgtc gttcggcagg tttgaccagt acatgctgcc
     2341 gtactggcag cgatcgattg ccgaagggat ggatcgcgaa ttcggcaagg aactgttgaa
     2401 gtgcttctgg gttcactgca acaccgccta cgatgcgcag atcagaagcg gcaatcaggg
     2461 aatcacggcc gggttcggac aattgataac cctgtccgga atgggtgccg acggccggga
     2521 catgacgaac gacttcactt acgcggtcct ggaagtcatc gacgagatgt ccccgatact
     2581 tgaaccgaag ccgaacgtca ggctgcatcg gggtacgccg ccggaattga tggacaagat
     2641 cgtccagatg atttcggaca gccagggcgc gcccttcctg ctgaatttcg atgaacgttc
     2701 catggcggga atgcttcgcc aggctgaact tgcaggcatg aaggacagga tcaacctgga
     2761 caacgtccac gaatatgccc cggtcgggtg tctggagaac acgatggtcg gcaacgaccg
     2821 gtcagggacc gttgacaaca acctgaacct ggtcaaggcg gtcgaactgg ccattaatgg
     2881 cgggcgcgac ctgctgcctt acgttgacca gataacaggc gtcgcggaac cggtgaccca
     2941 ggatgggccg gataccggag atgcgacgac tttcagtgat ttcgaggaat tctgggccgc
     3001 gtacgagcgg cagaccgcct atatcattga tcgttgcgca agcacttacg aggcgtcgga
     3061 aagtgtaagg gcccgattct ttcagacgcc ttatctgtcc tgcctggtgg ccggctgcgc
     3121 ggaaaagggg cgtgacatca accagggtgc cgccgaaatc aacctgacca cgctggaagc
     3181 cgtcaccttc gcgacgacgg tcgattcgtt gctggcggtc aagtacctgg tcttcgatcg
     3241 gaagatctgc acgatgggga agttgatcga tgccctgcgc gcgaactggg ccgggcacga
     3301 ggttcttcag gcacaggcca ggaacaaggc gcccaagtac ggtcgtgacg atgacgaagc
     3361 cgatgcgatg gcggttcgtg tcatgaacct gtggtgcgac ggggcatgga agcatgtcac
     3421 gaaatccacc ggccggcggt tccgcccggg gatgctttca tggaattact gggccgggga
     3481 cgggtatgtg gtggttgcca ccccggacgg tcgcccgcgt ggacagttcc tttcaaacgc
     3541 gatctgtccc agcaacgggg ccgacatcaa cgggccgacc tcgaacagca acagcgtggg
     3601 caaggccctg ggcggaaagt ccgaagatca ggtcgggaac taccgtggat acgtcaacct
     3661 gctgccaaac ggcgccagtc acaccatcac gttccacccg gccgtcatcc aggatccgga
     3721 gcattgcgaa aagttcaagt cgttcctgca cggatacatg gaaaacggcg gcacggccct
     3781 gcagatcaac atcctggatc cggacatgct caggatggcc cagaagcatc cgcaggatta
     3841 tcggcacctg cttgtgcgcg tcaccgggta caacgcgtac ttcgtaagca tcgggcgcga
     3901 actgcagaac gaggtgattg ccaggatgag tcaccagcgc ttctaggaac ccaggtgccc
     3961 cggggcttgt atgggcggac tggaagcgtc cgcttcaatt gtgtcggatg aaatcatgaa
     4021 ggatggcctg gtgcttgaaa tccagcggat gtcaacggaa gacgggcccg gtctgcggac
     4081 aaccgtgttc ctgaagggct gtccgctgcg ctgctcgtgg tgccacaacc cggaaagcat
     4141 cagtccgcag ccggagttgc agtggattcc ggttcgttgc atcgaatgcg gaacgtgcgt
     4201 tggattttgc agccgcggcg ctttgtcacg cggtgtcgaa ggtgtggtca tcgacagggg
     4261 tgcatgtgcc ggatgcgggg cctgcgccga tgcctgtccg accaacgcgc tggagatctg
     4321 ggggcgccgg atgaccgccc aggcccttgt ccgcgaggtc gcgaaggacg ccgcatactt
     4381 ttcccgatcc ggcggcgggg tcaccctttc aggcggcgag gcgacggccc agtcggaatt
     4441 ctgcatggac gtgctgcggg gcctgcgcgc atccgggttg aacaccgcac tggacacatg
     4501 cggcctttgc aggcccgaaa cattgcaggc gatgctgcct ttgtgcgacg tcgttcttta
     4561 tgacgtcaag atgatcgatt cgaaccttca ccgccagttc tgcggtgctt caaacgaagt
     4621 catccttgaa aacctgcgcc ttgtcgcgag cgcggttgtc ggatccgccg gtgcaacaag
     4681 cctgtgggtg cgaaccccgc tgatccccgg ggcgacagcg acgattgata acatgaaggc
     4741 cgtcgggggc ttcctggcga ccctgccgcc ggggtcggtt tcccgttggg acctgtgcgc
     4801 gttcaaccat cttggacggg acaagtacgt ccgactgggt atcgactggc agtatcggga
     4861 tatgccactg atgaccgctg ctgaacttga tgcaatgaag gcggcggcgc agtcgtccgg
     4921 tgtcgatccc gcgattgtcc ggacttccgg tgccaccagg cttcaggaat gattggggga
     4981 tgattatgaa caccttgtcc gaaggcgcca tgaaggcatt gaatgcgggg acgaagcttg
     5041 gcctgatcgc gaccgtggat ccggccggtc ttccgcatat caccctgatg acaagcctgg
     5101 aagcgaagga tccgaaccac ctgatgttcg ggcagttttc gtccgggaag tcgaaagagc
     5161 atctgcgatc caacccgttg atggcctttg tcgcggtcac tctggacaag cagactttgt
     5221 caggccacgg cagatacacc cacagtgtcc gtgaaggtgc cgattacgag cactacaaca
     5281 tgcagccgat gtttcggtac aacacatatt tcggaataaa cacggtgcat tatctcgatt
     5341 tgctggacgc gaccgacgtg gccccgctgc ccatgtcgcg aatcgttccc gcggccataa
     5401 ttacgcgcct tgtcaagggc gtccttgccg acggttcccg gccgcaaccc ctgacttcga
     5461 tggcgcgcga ccttttcaac cggctggatt cgttgaaatt catggcatac gtcgatgatt
     5521 ctggaatgcc gcagatgttt cccgtgatcc agtgccaggc agccgacgcc ggccgccttg
     5581 ccttttcaac gattgccggc gggccgcagc ttcggtcagt cccggttggc gccaccgtcg
     5641 ccgttttcgc catgaacatg atgatggaga gcatccttgt ccgtggaacc ttcaacggcc
     5701 tttcgcggcg ggccgtcttt cagtcaggca ccgtcgatat cgactgggtc tataactcga
     5761 tgcccccgtg ccacggccag atctggcccg aagtcccgct tgaaccagtc gagtcattct
     5821 gatcagtttt ttgtggtcgc gcattcgaag ccggtggtta tccggcatcc gcagcccgtc
     5881 agggaagtgt ggattcggcc tggatcgcgg ttgccttgaa gctgatgtcg gtcgcctgct
     5941 gaagatagat gctgtccgtg tcccattcaa acgttgccgt tgccgcgtga atcgatgtgc
     6001 cgtcgggcga tatcgccagg gacggcggca gtgcaccgat cagcgggctt ggacttttcg
     6061 tctggccatc ctgtgtaagc agggttctga cggtccactt cctgccgttt cgcaccgcat
     6121 accttatctg accgtcgatc gcgtttgccc agttgttgcc gtcgtgccac tgggccttgt
     6181 ccatgaagac gacatggggg cggtcagagg tgtccatgaa gattgacggc gagattccgg
     6241 tcatctttgt tccgtccgaa ccggcatacc catcgacttc cgtgacgacg gtttcgttgc
     6301 accatccgga ttgcccgctg ccgtaaaggc gaagctgggc cgacttgatt gaccccgtga
     6361 ttgcccgaac aaagtatgtg gccgcgatca gcgggtgccc gttcgaatcg atcgccaggg
     6421 ccgcgtcggt ggcgtattcg tcgttgggat cgccccattt cgattcctgg atcagatctt
     6481 cgctccattg cccatccatc ggaagggtgc cgtggtaaag ccccatgtcg cacgggtcgc
     6541 acggaatgct tgtcttgctg taaacggcat gaatggtgcc gtccggcgct tccttgatgg
     6601 aaaagaggtc ggtgacggaa ggcaggtcga accgg
//
LOCUS       DAOPPA010000129         6621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12747_length_6621_cov_3.784648, whole genome shotgun sequence.
ACCESSION   DAOPPA010000129 DAOPPA010000000
VERSION     DAOPPA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12747_length_6621_cov_3.784648"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..3218)
                     /locus_tag="PKH54_05360"
     CDS             complement(108..3218)
                     /locus_tag="PKH54_05360"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC99351.1"
                     /translation="MDNRFRRFPGWRLSFLSCIAVMAAFAGGCGDGGSLAELDWDQWD
                     SPSAEYRPWVRWWWPGNDVDDQELQREVALLAANGFGGAEVNSMSAALDWEPDEAEVA
                     RRFDWGSELFWSRVRLAAEAAQANGIMLDLTVGSGWPSGGPHVTIDDSMRTLLWSEFP
                     VTGPKSVTIEIGEPPKDPFYEVAALAEGIGEPMARWLPEGCTTVEVLAARKIGGIRNP
                     DVWDLTDQVNLDDESVTVLTASLKPDGKLTWDAPAGDWLIVAFHSCPDGQFVHLNAYA
                     EPKEAFVFDHFDVDQVVRGAEVLAGQDAGLGDLYGTAIRGLFVDSFEMSTERFFPADF
                     LQKFQEMRGYDVRPWLPAVVAPGADNYVFDGAGIANKCPFVFGEMDGRIARDYQKTVS
                     DLFIDRYIGTSTTWASARGMKFRIQPYGLHIDSIRAAGVASVPETEQLYGGGSELSLK
                     VMASGAHLYGRNVVSAESLVSAGRDYMSTPTKIRAWLDKLYTSGVNQAVYHGFPFKTG
                     LDKYGETDWHAFSSPYSGLGTYSENISEANPFWPFIGTINRYAARVQYMLRQGTPRAD
                     VLVYYPYLGIPASIMSLRDHQETLFKGQFGDENPRGQEGMLEMAQGLFGGFNPSDSTW
                     WMGAVWPVMQAVENLGYTWEWINDDALASAQVVDGRLVVGDASYGSVVVMRAPWMEKA
                     AAQNLAAMAADGLPVVVVGDAPAVQPGFHDYEAGDAAVDAAFDALSGLPNAASVPVVE
                     TPSTVENGNPQLFTADGLAAALMTAGLRSAFDISSRGATVRTIARDMGDARIWFIRNP
                     SDQDQEVQVTPDVDCGGGVVADAMEGWVAPLNLQAGGMFTVAIPAWTSRFVICGMPAP
                     ADLKSRAGLAPLSGEIHDVLTLDGWSLLVEGDDVVDGAYGSTENALGDWRDVEALKHC
                     SSQGLYQATFTSGFEFLWDVPTEERTALYIDLGIVHGAAAVSINGSGPYEVISEPYRL
                     DVTQYLMSGENEISVVLVPALRNRLVGLGLAKDPTAAQFDKKGDTIVATGLVGPVTLE
                     TIVGRW"
     gene            complement(3392..4396)
                     /locus_tag="PKH54_05365"
     CDS             complement(3392..4396)
                     /locus_tag="PKH54_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99352.1"
                     /translation="MTGFLVSSRQNCNRRCLMVLQAAIQMFALGVLVASVACGGGESG
                     DDNGSGNPDSVENIDVVGDNLLPGDQGDFDARDPGGDSVLDAGPDIDAGADADAAVPD
                     VPDVVQKDVQDIHGSDADPDLPNETVVGDDGNPSDVTPDSSQPSCSGSCQFGVDVDSC
                     DFGYLICGCDDDNQWREYDCREVCIEDGLLGGECVPQLGMLVCDCTSDCEDLQAISDD
                     CYYGLYTECTCAPEDPCGWSENGICNSGCLLEVPEFFDDFMDCRCLGACEYESFLGFC
                     DNDGDLCFCHEDEYQDYVSCPDYCQSLGMTTGKTESCTYELMEYLGDYEFLPTCDCVE
                     "
     gene            complement(4398..5564)
                     /locus_tag="PKH54_05370"
     CDS             complement(4398..5564)
                     /locus_tag="PKH54_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007948276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOC99353.1"
                     /translation="MESAERLCGIEPFYVMELLARAKELEAQGRDIIHMEVGEPDFPT
                     PEPVRRAGAQAILDGRMFYTNALGLPELRQAISDFYRERYRVDVPASRIVVTAGASGA
                     LVLALACLANPGSEWLLPDPGYPCNRHFVKSFDAVPVGMPVGSGTGFQPTVDDLARHF
                     NNRTAGVMVASPANPTGTMLSAEAIGSIAAFTRDRGLHLIVDEIYHGLTYDGDAPTAL
                     AAGDDIFVVNSFSKYFQMTGWRLGWLVVPDRFVRDIEKLAQNLFIAASTAAQHAALAA
                     FHPETIAILEERRAEMKRRRDFLVPALESVGLKIPVRPSGAFYIYADCSGLTDDSDCF
                     CRKLLEDVGVAVTPGKDFGINQPERHIRLAYANQISRLSEAVDRIRDFVSRQEV"
     gene            5832..>6621
                     /locus_tag="PKH54_05375"
     CDS             5832..>6621
                     /locus_tag="PKH54_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99354.1"
                     /translation="MTSLSRLTAGLCFIFLAATGCSSQVGQNPVRTLRASAGDGASAS
                     IDVDSSLGEASVTFTRTSDADGSVTGDRMNVSGTYLLNFSGSATGDAVLTIPLAASLL
                     PDEFSPASLTVERQDPLTGDWVPAASLTDYDVKSREISLVVPLEPESRSSTARSASMV
                     SCALTVAEASYRVGLALWTNSMEARIPGSAFVITYYPHAAGSDWTVPDDASWKSSSGR
                     ATDPATPDYIEDLDAALNDAYAKLLAVSDGTDAIFSPLTDDQRHV"
BASE COUNT         1382 a         2118 c         1912 g         1209 t
ORIGIN      
        1 ggaaggggtg aagggggagc cgcggaaggg tttaccgccg accgccaacc atcaccgggc
       61 gtactccctt tcctatgagg ggatggctca gcgttgcgtc gcggctgtca ccaccggccg
      121 acgatggttt caagggtgac cgggccgaca aggccggtcg cgacgatggt gtcgcccttt
      181 ttgtcgaact gggccgccgt cggatccttg gcaaggccaa ggccgaccag gcgattgcgc
      241 agggccggaa ccagaacgac ggaaatctcg ttttcacccg acatcaggta ctgggtgacg
      301 tccaacctgt aaggctcgga tatcacctca taagggccgg atccgttgat gctgaccgcc
      361 gcggccccgt gtacgatgcc caggtcgatg taaagcgcgg tcctttcctc ggtcgggacg
      421 tcccacagga attcgaagcc ggacgtgaag gtcgcctggt aaagcccctg gctggagcaa
      481 tgcttcaacg cctcgacgtc acgccagtcg cccagggcgt tttcggtcga accgtatgcg
      541 ccatcgacga cgtcgtcacc ttcaacgagc aggctccagc catccagggt caggacgtca
      601 tggatctccc cggacaaggg cgccaaccct gcccggcttt tcagatccgc cggagccggc
      661 atcccgcaaa tcacgaaccg tgaagtccac gccggtatcg ccaccgtgaa catgccgccg
      721 gcctgcaaat tgagcggtgc cacccagcct tccatcgcat ccgcgaccac gccgccgccg
      781 caatcgacat cgggggtgac ctggacttcc tggtcctggt cggacgggtt gcggatgaac
      841 catatccggg cgtcgcccat gtcgcgggcg atcgtgcgga cggtcgcgcc gcgggatgaa
      901 atatcgaagg cggaacgaag gccggctgtc atcagcgcgg cggcaagccc gtcggcggta
      961 aacagttgcg ggttgccgtt ctccaccgtc gaaggcgttt cgacgacagg aaccgaagcg
     1021 gcattcggca ggcccgaaag cgcgtcaaac gccgcgtcaa ccgcggcatc accggcctcg
     1081 taatcatgga agcccggctg caccgccggt gcatcgccaa cgaccacgac cggcagtccg
     1141 tccgcagcca tggccgccag gttctgcgcc gcggcctttt ccatccaagg cgcgcgcatt
     1201 accacgaccg agccgtaact ggcatcgccg acgacaagcc gcccatccac aacctgggcg
     1261 gaagccagcg cgtcgtcgtt gatccattcc caggtgtagc ccagattttc gaccgcctgc
     1321 atcaccggcc aaaccgcgcc catccaccag gtcgaatcac ttggattgaa gcctccgaac
     1381 agcccctggg ccatctcgag catgccttcc tggccgcgcg ggttttcgtc gccgaactgg
     1441 cccttgaaca gggtttcctg gtggtcgcgc agcgacatga tcgaagccgg aatgcccagg
     1501 tacgggtaat agacaagcac gtcggccctg ggcgtgccct ggcgcagcat gtattgcacg
     1561 cgggccgcgt accggttgat ggtgccgatg aacggccaga aaggattggc ctcgctgatg
     1621 ttttccgaat acgtcccaag gcccgaatat ggcgacgaaa aagcgtgcca gtcggtttcg
     1681 ccgtacttat ccagcccggt cttgaacggg aatccgtgat agacggcctg gttcacgccc
     1741 gacgtgtaca gtttgtccag ccacgcgcgg atcttggtcg gcgtggacat gtagtcacga
     1801 ccggccgaaa ccagcgattc agcggaaacg acgttcctgc cgtacaggtg cgcgcctgac
     1861 gccatgacct tcagcgacag ctcggatccg ccgccgtaaa gctgctcggt ttcgggaacc
     1921 gatgccacac cggccgcacg gatcgaatca atgtgcagcc cgtatggctg gattcggaac
     1981 ttcatcccgc gggctgaagc ccatgtcgtg ctggtgccga tgtaacgatc gatgaaaagg
     2041 tcggaaaccg tcttctggta atcccgggcg atcctgccgt ccatttcgcc gaacacgaac
     2101 ggacacttgt tggcaatccc ggcgccgtcg aacacgtaat tgtccgcgcc aggcgccacc
     2161 accgccggca gccacggtcg cacgtcgtaa ccgcgcattt cctggaactt ctgaagaaag
     2221 tctgccggga agaagcgctc ggtgctcatc tcgaaactat cgacgaacaa cccgcggatt
     2281 gccgtgccgt acaagtcgcc aagaccggcg tcctggccgg ccaggacttc ggccccgcgc
     2341 acgacctgat cgacgtcgaa atggtcgaac acgaacgctt ccttcggctc ggcgtacgcg
     2401 ttcaggtgga cgaactggcc gtccggacat gaatggaagg caacgatcag ccagtcaccg
     2461 gcaggcgcgt cccaggtcag cttgccgtcg ggcttcaggc ttgcagtcag cactgtgacc
     2521 gactcatcgt ccagattcac ctggtcggtc aaatcccata cgtcgggatt gcggattccg
     2581 ccgatcttcc tggcggccag gacttccacg gtcgtgcaac cctcgggcag ccaccgggcc
     2641 atcggctcgc cgatgccttc ggccagcgcc gcgacctcgt aaaacggatc cttcggaggt
     2701 tcgccaatct cgatggttac gctcttcgga ccggtaaccg ggaattccga ccagagcagc
     2761 gtccgcatcg aatcgtcgat ggttacatgc gggcccccgc ttggccagcc ggagccgacc
     2821 gtcagatcca gcatgattcc attggcctgg gctgcctcgg cggccagcct gacacgcgac
     2881 cagaacaact cggaacccca gtcgaaacgg cgcgcgactt cggcctcgtc cggttcccag
     2941 tcaagcgccg cgctcatcga gttgacctcg gctccgccga aaccgttggc ggcaagcaga
     3001 gccacctccc gctgaagttc ctggtcatca acgtcgttcc caggccacca ccagcgaacc
     3061 cacggcctgt attcggccga aggcgaatcc cactgatccc agtccagttc cgccagcgaa
     3121 ccgccatcgc cgcagccgcc ggcgaatgcc gccatcaccg cgatgcagga cagaaacgaa
     3181 aggcgccatc caggaaacct tcgaaaacga ttatccacag aaacctccgg aaccaggcgt
     3241 gatggcggca ggtcgccgca tgggagccgt ttcccccgat tatagactta tgcgaaggat
     3301 ttgcgaaaac ggacagaagg tcatctgtgg catgtggctg gagtcggatt gcggcaggaa
     3361 tatcaaacag gcggactgtt cattcaaccc attactcaac acagtcacag gtcggcagga
     3421 attcataatc gcccagatat tccatcagct cgtatgtgca tgattcggtc tttccggtgg
     3481 tcattccaag tgactggcag taatccggac aacttacgta atcctggtat tcgtcctcgt
     3541 gacaaaagca caagtcaccg tcgttgtcgc agaagcccag gaagctttca tattcgcacg
     3601 cgccaagaca ccggcagtcc atgaagtcat cgaagaactc tggaacctcc agcaggcaac
     3661 ccgaattgca gataccattt tcagaccagc cgcaaggatc ttccggagca catgtgcatt
     3721 cagtatagag tccatagtag cagtcgtccg agatcgcctg aaggtcttcg caatcgcttg
     3781 tgcagtcgca aaccagcatg ccgagttgag gcacgcactc cccgcccagc aggccgtcct
     3841 cgatgcacac ttcgcgacaa tcgtattcgc gccactgatt gtcatcgtca caaccgcaga
     3901 tcaggtaccc gaaatcgcac gaatcgacat caaccccgaa ctggcatgaa ccggaacacg
     3961 acggttggga ggagtcggga gtgacgtcag atggattgcc gtcgtcacca acaacggttt
     4021 cattcggcaa atcgggatct gcatcggaac catgaatgtc ctggacatcc ttctggacga
     4081 catccggcac atcaggaacg gctgcgtccg cgtccgctcc ggcatcgatg tccggccctg
     4141 cgtcaaggac agagtcacca ccgggatccc tggcatcgaa atcgccctga tcccccggca
     4201 acaggttatc ccccacgaca tcaatgtttt caacggagtc tgggttgccc gaaccgttat
     4261 catcgcctga ttcgccgccc ccgcaggcga cagaagcaac cagaacgccc aaagcaaaca
     4321 tctgaattgc ggcctgaagc accataaggc accgacgatt gcaattctgc cgtgaggaca
     4381 ctaaaaatcc tgtcatgtca gacctcctgc ctggaaacga aatccctgat tcgatcaaca
     4441 gcctctgaaa gccgtgaaat ctggttggcg taagcaagcc ttatgtgccg ctcgggctgg
     4501 ttgataccga aatccttgcc gggcgttacc gcgacgccga catcttccag aagcttgcgg
     4561 cagaaacagt cgctgtcgtc cgtaagcccg ctgcagtcgg cataaatata aaacgcgccg
     4621 gacgggcgca ccgggatctt cagcccgacc gattccaggg ccggaacaag gaaatcgcgg
     4681 cgccgcttca tctcggcacg ccgctcttcc aggatggcga tcgtctccgg atggaaggcc
     4741 gcaagcgccg catgctgcgc ggcggtcgaa gcggcgataa aaaggttctg ggccagtttc
     4801 tcgatatccc tgacgaagcg atccggcacg acaagccagc caagccgcca gcctgtcatc
     4861 tggaaatact tggaaaaact gtttaccacg aagatgtcgt cgccggccgc cagggccgtc
     4921 ggcgcatcac cgtcgtatgt aaggccgtga tagatttcat cgacgatcag gtgcaggccg
     4981 cgatccctgg tgaatgcggc aatcgaaccg attgcttcgg ccgaaagcat cgtgccggtc
     5041 gggtttgccg gcgacgcgac catcacgccg gccgtgcgat tgttgaagtg acgcgcaaga
     5101 tcatcgactg tcggctgaaa tcccgttccg gaaccgaccg gcatgccaac cggcaccgcg
     5161 tcgaacgact tcacgaagtg acggttgcac ggatacccgg gatccggcag cagccactcg
     5221 ctgccgggat ttgccaggca cgcaagggcc aggacaaggg cgccagaagc gccggccgtg
     5281 accacaatcc gggaggctgg aacatcgacc cggtaccttt cccgataaaa atccgaaatg
     5341 gcctggcgaa gttccggcag cccgagtgcg ttcgtgtaaa acatcctgcc gtcaagtatg
     5401 gcctgcgcgc ccgccctgcg gacaggctcc ggggttggaa agtccggctc gccgacttcc
     5461 atgtgaatga tgtcacggcc ctgcgcctcg agttccttgg ctctggcaag cagttccatc
     5521 acatagaaag gttcgattcc gcacaatctt tcagccgatt ccatcaccac gcctccgcga
     5581 aaggacggtt ccggacaatt cctgtcggaa ccggccggag tataacgccg aaacgactta
     5641 tgggaacatg gactcaaccg gctccagaaa ttcgtccaga acggccggtg ctacttatcg
     5701 ccggcgggct tcggctgctt cagtgaattc ccgcgcgtcg acattttccg tgccgccgct
     5761 tttcgggata caatccggcc ttcgattggt cgcgggcgat ccgtttatcg ttgcaggaaa
     5821 gggggggctg tatgacttcg ttgagccgct tgacggccgg attgtgtttc atatttcttg
     5881 ccgcaaccgg atgctcgtcc caggtgggac aaaacccggt tcgaaccctg agggcttctg
     5941 cgggggacgg cgccagcgca tcgattgacg ttgactcgtc gctcggggaa gccagcgtca
     6001 ccttcaccag aacgtcggac gccgacggca gcgtcaccgg cgaccgcatg aacgtatccg
     6061 gcacctatct gctcaatttc agcggatccg ccacaggcga cgcggtcctg accattccac
     6121 tggcagccag tctgcttccg gacgaatttt cgcctgcatc actgaccgtt gaacgccagg
     6181 atccgttgac tggagactgg gttccggcag ccagtctgac ggactatgac gtcaaatcca
     6241 gggagatatc tttagtggtg ccgctggaac cggaatcccg gtcgtcgaca gcgaggtcgg
     6301 cctcgatggt gtcttgcgcc ctgacggtgg ccgaagccag ctaccgggtc gggctcgcgc
     6361 tctggacgaa ctccatggaa gccaggatcc ccggatcggc tttcgtgata acctactatc
     6421 cgcatgcggc cggaagcgac tggaccgttc cggatgacgc gtcctggaaa agctccagcg
     6481 gccgggcgac agatcccgcg acccccgact acatcgaaga ccttgacgct gccctgaacg
     6541 acgcctatgc gaagctgctt gcggtgtccg atggaaccga cgcgatcttt tcgccgttga
     6601 ccgacgatca gcgccatgta t
//
LOCUS       DAOPPA010000130         6612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12766_length_6612_cov_3.518530, whole genome shotgun sequence.
ACCESSION   DAOPPA010000130 DAOPPA010000000
VERSION     DAOPPA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12766_length_6612_cov_3.518530"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..414
                     /locus_tag="PKH54_05380"
     CDS             25..414
                     /locus_tag="PKH54_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99355.1"
                     /translation="MGEARMAAEKVLELVPDESDPTHMAAVDLMENVARESPLTRKDK
                     RCFIATAAMDDVDAPEVEALRAFRDRVLFASTAGRVFVRIYYRCSPPLASYISRHRAV
                     RLAVRRYLVLPLAAMAGLSGDRRRSRR"
     gene            427..1329
                     /locus_tag="PKH54_05385"
     CDS             427..1329
                     /locus_tag="PKH54_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC99356.1"
                     /translation="MSLTEQIRKIIGFEQEAAPTPRGKILAVAARKGGVGKTTTTVNL
                     AVAMARYHGLDVLMVDMDGQGHVAEALSAVVRDQGGASLSDVMLSSGREVRRVDDLMD
                     IVVQTSEPRLWATPSDKGLAVTEGIMAGMIGREFLLRNVMRKAAARFDVIIFDCPPNV
                     GNLTLNAMVASSHVLVPCDMSILSMAAVGDIIGVIDTVNEQLGHDLEVLGVLPVRVDR
                     RNGKLTDQILKELSDNWGERLLSSRIPANSSLARAQVSGVSVFDFDSRSAGARAYRNL
                     AAEVLGRMGFRGVDAPAGRKFQKS"
     gene            complement(1283..2362)
                     /locus_tag="PKH54_05390"
     CDS             complement(1283..2362)
                     /locus_tag="PKH54_05390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC99357.1"
                     /translation="MQIESAWKSVLRPSGQWRSVIDFFCERCGRVFKAPDPAVCTEDG
                     IELRPLAARLPERGELIGKHYRVNKKVGDGGMCAIYSATDLTNDTEVAVKVLHPELAT
                     EPGQSESMLYEVSLSLSLDDENLVRCYDGGISRNGFHFMVMDLLEGRTLAQLLAIRGR
                     VCMEDAAEIFIGAARGLHAVHQSGHVHRDVKPENIFINGNATTRTIMAERVKLMDLGV
                     ARPANAPVRSMTVYGTPEYMSPEQVSGQSLDRRSDLYSLGVVLYEMLTGKPCFDGTDA
                     REVMLAHIRRQPPALPESLEVTPLGRILSGIVTSLLQKKPDDRPSSALEISRLIPLAD
                     IRRNTTSRLDFSFFETSAPPEHRLP"
     gene            complement(2415..3152)
                     /locus_tag="PKH54_05395"
     CDS             complement(2415..3152)
                     /locus_tag="PKH54_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99358.1"
                     /translation="MKDIDSIEISQILEEADEDMFDARRLGGRSAPAARYAASQSAEK
                     YLRALCEAVGRPAGIMWDILKIHDTVGDIAGLGAIRDSVELLSKFTTPARSGDGATAP
                     MKDVLHSVETVRWAVRKAFGIDEPAPVAPESGAVPCVSSEVIDSAVIPVDDDLIPTIL
                     PGDASGPDGDNQPVATEPVRPAAPDMQARNRREPGAGRSPEERNTSFVKTFLVCDRCG
                     VRIPRTRQTATGRVPCAMCGRPMKLQR"
     gene            complement(3183..4079)
                     /gene="rapZ"
                     /locus_tag="PKH54_05400"
     CDS             complement(3183..4079)
                     /gene="rapZ"
                     /locus_tag="PKH54_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOC99359.1"
                     /translation="MTSEHIVIVTGLSGSGKTAVIKAMEDLGFFCVDNLPLPLLDKIL
                     FLAETHQEISRICIGIDVREGPLLKSFRTEVGKIRNLGHKLEIIYLEAGQEALERRYS
                     ETRRRHPLQELAGSVRQGIERESELLGEIRTMADWVIDTTDLNVHDVRTLIHRAYDRS
                     ESSRMAVEVVSFGFRDGVPRDASYVFDCRFLRNPHFESELRPLTGADPAIVEFMEGTP
                     EWGDFIDRVSNMLRFTLPLHEREPRPLITIAFGCTGGRHRSVAAAEKVARILAESGFR
                     VKVSHRNRETARDIDGKGTTTE"
     gene            complement(4097..4396)
                     /gene="raiA"
                     /locus_tag="PKH54_05405"
     CDS             complement(4097..4396)
                     /gene="raiA"
                     /locus_tag="PKH54_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOC99360.1"
                     /translation="MKVNYTLRQFNASDALRELIESKLEKHIGQYLPEEHEIKVTLGM
                     EKSWATFDLMVQFKGETFKATEKATDIYPVLDVVIEKVARQVAKHRDMIVDKRKR"
     gene            complement(4540..6021)
                     /gene="rpoN"
                     /locus_tag="PKH54_05410"
     CDS             complement(4540..6021)
                     /gene="rpoN"
                     /locus_tag="PKH54_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOC99361.1"
                     /translation="MDIRQELGTRLQQRLIPTPQLQVAIRLLQLSRLELVDEIRQQLD
                     ANPALEEVEVNSESISLDEPSNQSPNLSQPAPDTSRPPDPDDQAAASERSEMSDQDSI
                     DWEKYVEGYNTFSHGPEIRVSNDEYPTVEATVGARSSLAEHLTWQLNLSDLPPLSREV
                     GRFIIGNINDDGYLEDCLDEEIALATDTTVEHVREVLTVIRAFDPPGVASRSLKECLL
                     VQAALNWPDNMLLRKMIENHLPDLERRNFQAVARGLRVGLDDVKELIGLLQTMEPRPG
                     RQYSSDDIIYVQPDVHVIKVGDEYVVVVNDDGLPRLKISQYYEKTLSRQTAGEAREFV
                     KDKLRAASWLIKSINQRQTTIRRVTESIMKFQHDFLEHGVEHLKPLVLRDVADDVGMH
                     ESTISRVTTNKYVHTPQGIFELKYFFNSKISGAWGVEDHSSMSVKSRIKALVDAERPD
                     RPLSDQEIVKILGDEGVIIARRTVAKYREALGIAPSSRRKELV"
     gene            6182..>6612
                     /locus_tag="PKH54_05415"
     CDS             6182..>6612
                     /locus_tag="PKH54_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HOC99362.1"
                     /translation="MPEWLLKGDASGSFDLNDIEAVRLILRGGSVVDWARLNLRDADE
                     VASFLLVNGFDVTVPDDQARLRSLLKKAVAYLEANFNYHFPEGLRSPSSIEALLLTAS
                     RDDEYQKLACVILKVMHVVNHVDAQQLRFRLAVPEDQLFQMA"
BASE COUNT         1174 a         1982 c         2018 g         1438 t
ORIGIN      
        1 cagcttcgac tcgccaaggg cgatgtgggc gaggccagga tggcggctga aaaggttctg
       61 gaactggtgc cggacgagtc cgatccgacc catatggcgg cagtcgatct gatggaaaac
      121 gtcgcccgtg agtccccgct taccagaaag gacaagcgct gcttcatagc gacggcggcg
      181 atggacgacg tcgatgcccc cgaggtcgag gcgctgaggg cgttccgcga tcgtgtgctt
      241 ttcgcgtcga cagccggccg cgttttcgtc cggatctact accggtgttc accccctctg
      301 gcgtcgtaca tttcgcgcca ccgggcggtg cgcctggctg ttcgcaggta tctggtgctg
      361 cccctggccg ccatggccgg attgtccggc gatcggcgcc gtagccgtcg ctgaaggagg
      421 tttgccttgt cgctgaccga acagatccgg aagattatcg ggttcgaaca ggaagccgct
      481 ccgacgcccc gaggcaagat ccttgccgtg gccgcgcgca agggtggagt gggcaagacc
      541 accacgaccg tcaaccttgc ggtcgcgatg gccaggtatc atggccttga tgtcctgatg
      601 gtcgatatgg atggccaggg acacgtggcc gaggcgcttt ccgccgtggt tcgggatcag
      661 ggcggggcgt cgctgtcgga cgtcatgctt tcttccggca gggaagtcag gcgggtcgat
      721 gacctgatgg acatcgttgt ccagacttcg gaaccccggc tgtgggccac gccttcggac
      781 aagggccttg ccgtgaccga gggaataatg gcgggcatga tcggtcggga attcctgctg
      841 cgaaacgtca tgcggaaggc ggcggcccgg ttcgacgtca tcatcttcga ctgccccccg
      901 aacgtcggca accttacgtt gaacgcaatg gtggcgtcgt cccacgtcct tgttccctgc
      961 gacatgagca tcctttcgat ggccgccgta ggcgatatca tcggcgtgat tgataccgtc
     1021 aacgagcagc ttggacatga tctcgaggtt ctcggagttc ttcccgtccg tgtcgacagg
     1081 cgcaacggaa agctgaccga ccagatcctg aaggagttgt cggacaattg gggggaaagg
     1141 cttctttcaa gccgcattcc ggccaacagt tcccttgccc gcgcccaggt ttcaggcgtt
     1201 tcagtcttcg acttcgactc acgttcggcg ggtgccaggg cttacaggaa cctggcggcg
     1261 gaagtcctgg gccgaatggg tttcagggga gtcgatgctc cggcggggcg gaagtttcaa
     1321 aaaagctgaa atccagcctt gaggtcgtgt tgcgcctgat gtcggcaagc ggtatcaacc
     1381 gcgagatttc aagcgcggac gatggcctgt cgtccggttt tttctgaagc agcgacgtga
     1441 ctattccgga aagaatcctt cccagcggcg tcacctcgag cgattcggga agggcgggag
     1501 gctggcgtcg gatatgggcc agcatgacct ccctggcatc tgttccgtcg aagcacggtt
     1561 ttccggtcag catttcgtaa agcacgaccc cgagcgagta caggtcgctc cgccggtcaa
     1621 gggactggcc ggagacctgc tccggactca tgtactccgg cgttccatag acggtcatgc
     1681 tgcggaccgg agcgtttgcc ggcctggcga cgccaagatc catcaacttg accctttccg
     1741 ccatgattgt gcgggtcgtt gcattgccgt tgatgaatat gttttccggc ttgacgtcgc
     1801 ggtgaacatg gccgctctgg tgaaccgcgt gcaggccgcg ggccgccccg atgaagatct
     1861 cggcggcgtc ctccatgcag acccgaccgc gtatcgcaag aagttgggca agagtgcgcc
     1921 cttccagcag atccatgacc atgaagtgaa agccgtttcg cgaaattccg ccgtcgtagc
     1981 atcgaaccag gttctcatca tccaggctca gggaaaggct gacctcgtag agcatcgact
     2041 cgctctgtcc cggttcggtg gcaagctccg ggtgcagcac cttaaccgcc acttcggtgt
     2101 cgttcgtcag gtcggtggcg ctgtatatgg cgcacatgcc gccgtcaccg acctttttgt
     2161 tcacgcggta atgcttgccg atcagttcgc cgcgttccgg cagcctggcg gccagcggac
     2221 gcagttcaat cccgtcttcg gtgcagacgg ccgggtcggg cgccttaaag accctgccgc
     2281 atctttcgca gaagaagtcg atgactgacc tccattgtcc agacggtcga aggacagact
     2341 tccacgctga ttctatttgc aatcaaggcc cccttccaaa ttggcgtcgg tcgggggcgt
     2401 caggttcggt cggttcagcg ctgaagtttc attggacgtc cgcacatcgc gcagggtacc
     2461 cggccggtcg cggtctgcct ggtgcgcggt attctgacgc cgcaccggtc gcagacaagg
     2521 aaagtcttta cgaaggacgt gttgcgctcc tccgggcttc ttccggcccc gggttcccgg
     2581 cggttccgtg cctgcatgtc aggggctgcc ggccgaaccg gttcggtagc gacgggctgg
     2641 ttgtcgccgt ccgggccgga agcgtcgccg ggaagtatgg tcgggataag gtcgtcgtca
     2701 accggaatca cggcggagtc gatgacttcc gaggagacgc acggaaccgc cccggactcg
     2761 ggggccaccg gggccggctc atcgattccg aaggccttgc gcaccgccca gcgaacggtt
     2821 tccactgaat gcaggacgtc cttcattggc gcggtcgcgc cgtcgccgga gcgggccggt
     2881 gtcgtgaact tcgaaagaag ctcgacagaa tcccttatcg cgccaaggcc ggcgatatct
     2941 cccaccgtgt cgtgaatctt gaggatgtcc cacatgattc cggctgggcg tccgaccgcc
     3001 tcgcaaagcg ccctcagata cttttccgcc gactgggatg cggcgtatct ggccgccgga
     3061 gcgcttctgc ccccgagccg ccgggcatcg aacatgtcct cgtcggcctc ttccaggatc
     3121 tgggatatct caattgaatc gatatctttc atataaatcc ttgttgctgc gacgatgcag
     3181 cgtcattccg tcgttgttcc ttttccgtcg atgtccctgg ccgtttccct gttgcgatgg
     3241 gataccttca ccctgaaccc ggattccgca agtatccgcg ccaccttttc ggccgccgcg
     3301 accgaacggt ggcggccacc ggtgcatccg aacgcgattg tgattaaagg tcgcggctcc
     3361 ctttcgtgaa ggggcagcgt gaaccgcagc atgttcgaca cccgatcgat gaagtcgccc
     3421 cactccggcg tcccttccat gaactcgaca atggcagggt cggcgccggt aagtggccgc
     3481 agttcgcttt cgaaatgggg attcctgagg aagcggcagt cgaacacata ggatgcgtca
     3541 cgcggcacgc catccctgaa cccgaaagag acgacctcaa cggccatgcg gctggattcg
     3601 gaacggtcat aggctcgatg tatcagggtt cgcacgtcgt gaacgttcag atccgtcgtg
     3661 tcgatgaccc agtcggccat cgtccggatc tcgccaagaa gctcgctttc ccgctcgatt
     3721 ccctggcgca ccgaaccggc aagttcctgc agcggatgtc ttcgcctggt ttccgagtac
     3781 ctccgttcga gggcctcctg cccggcttcc agataaatga tttccagctt gtgcccaaga
     3841 ttccggatct tgccgacttc cgtcctgaag gatttcagca gtgggccctc cctgacgtcg
     3901 atgccgatgc agatcctgga aatttcctgg tgggtttccg caagaaacag gatcttgtcc
     3961 agcagcggca gcgggaggtt atcgacgcag aagaacccga gatcctccat ggccttgatt
     4021 acggccgtct tccccgagcc cgagagcccg gtcacaatga cgatatgttc cgaagtcaag
     4081 gtggggacgc ctccggtcaa cgcttgcgct tgtcaacaat catgtcgcgg tgtttcgcga
     4141 cctgcctggc gaccttctcg atcacgacgt ccaggacggg atagatgtcg gttgcctttt
     4201 cggtggcctt gaacgtttcg cccttgaact gaaccatcag gtcgaaggtg gcccaggatt
     4261 tttccatgcc cagggttacc tttatctcgt gttcttcggg aagatactga ccgatgtgct
     4321 tttccagctt cgactcaatc agttcgcgaa gcgcatcgga tgcattgaac tggcgaaggg
     4381 tgtagttaac cttcatggtg actcctttcc ggcgccgccc cgtttccctg ggctgcgctc
     4441 gaaactcggg cggcgcaata tccgcccgaa agggttgcgc gacctggcag gccgcgcgat
     4501 atcaaacacc cgccaggctg catgggccgg gcagggagcc tacacaagtt ccttcctgcg
     4561 tgacgacggc gcgatcccca gggcttcacg gtacttcgcc accgtgcgcc ttgcaataat
     4621 cacgccctcg tctcccagta ttttcacgat ttcctgatcc gacaagggcc gatccggtct
     4681 ttccgcgtca acaagcgcct tgattctgga cttgacgctc atcgatgagt gatcctcgac
     4741 cccccaggcg ccggaaatct tgctgttgaa aaagtacttc agttcgaaga tgccctgcgg
     4801 cgtatgcacg tacttgtttg tggtgacccg gctgatggtt gattcgtgca tgccgacatc
     4861 gtccgccaca tcccgcagca ccaggggttt caggtgttcg accccgtgtt ccaggaagtc
     4921 gtgctggaac ttcatgatgc tttccgtcac ccgccggatc gtcgtctggc gctggttgat
     4981 cgacttaatc agccaggatg cggccctgag cttgtccttg acgaactccc tggcctcgcc
     5041 cgccgtctgc cgtgaaagcg tcttttcgta atactggctg attttcagtc gtggaaggcc
     5101 gtcgtcgttg acgacaacga catattcgtc cccgactttg attacatgca cgtccggctg
     5161 gacatagatg atatcgtcgg atgagtactg gcggcccggc ctgggttcca tcgtctgtaa
     5221 cagtccgatg agttccttca cgtcgtccag accgacgcga aggccgcgcg ccaccgcctg
     5281 gaagttgcgc ctttcaaggt cggggaggtg gttttcaatc atcttccgca gcagcatgtt
     5341 gtccggccag ttcagtgcgg cctggacaag aaggcattcc ttcaggctgc gcgaggcgac
     5401 gcccgggggg tcgaaggccc tgatcaccgt caggacttcc cgcacatgct cgaccgtggt
     5461 gtcggtggcc agggcgatct cctcgtcaag gcagtcctca agatatccgt cgtcgttgat
     5521 gttgcctatt atgaagcggc ccacctctct ggaaagcggc ggcaggtcgg acaggttcaa
     5581 ctgccaggtc aagtgctcgg ccagggaact gcgcgcgccg acggtcgcct caaccgtcgg
     5641 gtattcgtcg ttggaaaccc ggatctcggg tccatgcgag aaggtgttgt atccctcgac
     5701 gtacttttcc cagtcgatgc tgtcctgatc ggacatttcg ctgcgttccg aggccgctgc
     5761 ctgatcgtcg ggatccgggg gccttgaagt gtccggggcc ggctgactca ggtttggcga
     5821 ctgattcgac ggctcgtcaa gggagatgct ttcgctgttg acctcgactt cctcgagggc
     5881 gggattggcg tcaagctgct ggcggatttc atcgacaagc tcgagacgtg acaactgcag
     5941 cagcctgatg gcgacctgca gttgcggggt cgggatcagt ctctgttgta atctggttcc
     6001 cagctcctgc cggatatcca taggctatct tccgttactg cagccggcaa ggcccacatc
     6061 acgcgaaccg tcgaatcaaa gctagcaggg gtctgcaagg ttgtaaaggt taatgctccg
     6121 gactacaatc cgcccgttgg ttgggcacac ccggcggtcg ccggaaggaa gcgtgtcacg
     6181 tatgcctgaa tggctcctga agggtgatgc gtcaggttcg ttcgacctga acgatatcga
     6241 ggcggttcga ctgatcctca ggggcggctc ggtcgttgac tgggcgcgcc tgaacctccg
     6301 tgacgcggac gaggtcgcga gtttcctgct ggtcaacggg ttcgatgtga cggtgccgga
     6361 tgaccaggcc aggctgcgga gcctgttgaa gaaggccgtg gcatacctcg aggccaattt
     6421 caattaccac ttccctgaag gtcttcgcag cccctcgtca atcgaggcgc ttctgctgac
     6481 ggcctcccgg gacgacgagt accagaagct ggcctgtgta atcctgaagg tcatgcacgt
     6541 cgtgaatcat gtcgatgcgc agcagcttcg attccgcctt gccgttccgg aagaccagct
     6601 tttccagatg gc
//
LOCUS       DAOPPA010000131         6601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12787_length_6601_cov_4.022915, whole genome shotgun sequence.
ACCESSION   DAOPPA010000131 DAOPPA010000000
VERSION     DAOPPA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12787_length_6601_cov_4.022915"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /gene="rplI"
                     /locus_tag="PKH54_05420"
     CDS             <1..224
                     /gene="rplI"
                     /locus_tag="PKH54_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOC99363.1"
                     /translation="TSVNIVREAGEEEKLFGSVTNRDIAEALAAEGIVVDHRVILLEN
                     PIKTLGSFKVEVKLTTEVRAEVKVRVVAK"
     gene            221..961
                     /locus_tag="PKH54_05425"
     CDS             221..961
                     /locus_tag="PKH54_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOC99364.1"
                     /translation="MTIPRLFESGRELRPGSSFTPEIDVMHYLRRVLRLKPGDGVLVL
                     DGGPVAWLGTLAGSDDGLGIAISGPAEGVRVVPDGRRICALVPALKGDRHEFAIQKAT
                     ELGVTELRIYFADRSIPRPSARESAKLARWMKVAEEACRQCDRASVPLVGIYDDLGCA
                     TGELDIYADRYLLSEIGTPEPLARIATGTRPAVVAIGPEGSFTEAERGMLLQAGFVPA
                     GLGPRVLKAETAVIAAVMAVQVAGGDIG"
     gene            958..1596
                     /locus_tag="PKH54_05430"
     CDS             958..1596
                     /locus_tag="PKH54_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOC99365.1"
                     /translation="MSKSEPGGHDEFVVRVAASTLRVLAGLIDLAVPAGLTVLACFLS
                     ETPDLSRLPPRYWNYLDYTVDVFNSQPSVFIVPALYFVALYVLGTVVFVSTIGNTPFS
                     RLVGIRVTDRRGRGIGPIRSFFWTVCGLLLGVVAFVGPLWTIVDPKRRMLHDILAGVV
                     VVFGRPVVTMVTADPDEAPVDDIFPAPEEVKGDVNGRSAGGSAPWHAEGGNW"
     gene            1593..2393
                     /locus_tag="PKH54_05435"
     CDS             1593..2393
                     /locus_tag="PKH54_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99366.1"
                     /translation="MISFRRFNLLVFSVLATLTFSGAVASAAPKDSAMPARPTIVDYS
                     GKMVDEAGRPVSGIYPIEFKLYSPGSKKPVFSDRMWVAVVDGAYTVGIGSQKLLPRGQ
                     DFTKLMLGVEISGVELSRQVFMDKDTARKVQQDMEKKAAEVSDTKLSVDSARAGSPAS
                     NTGSVKYADSAGYAVSSDHAKNADRIQNLTVDDLVRKVVEEGGGGSSGGSVTIGRTRR
                     YGDRVGGPGGDIEYNEVCPKGYVMVGVRGGAGKFLDSIQIVCAPLGAE"
     gene            2426..4186
                     /gene="aspS"
                     /locus_tag="PKH54_05440"
     CDS             2426..4186
                     /gene="aspS"
                     /locus_tag="PKH54_05440"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOC99367.1"
                     /translation="MDGVRTHNCGELRESDIGAQVMLQGWAASVRDHGGCAFINLRDR
                     FGTTQIKFDQALNPAAFDKAVGVRSEFVIQVTGTVVGRGANANLKIPTGGIEVEAGSI
                     EILSSAKPLPFQIADDVDAQEVTRLKYRYLDLRRNQLNSNIVMRSKVCKMVRDYLETR
                     DFLEIETPILMKSTPEGARDFLVPSRVHPGQFYALPQSPQTFKQLLMVSGFDRYFQIA
                     RCFRDEDLRADRQPEFTQIDIEMSFAVPELIYEIVEGMLKHVWKHALGIDIQTPFLRM
                     PYSEAMEKYGCDKPDMRFGMEMVTITDEVAGSGFKVFSDTAAAGGVVTAIKVPDAEKW
                     SRKDIDALTQTATDNGAKGLAWFKIGESEWSGSAAKFLSDGDKSAIATKMQAGPGDLL
                     LVVADRPNKARQAMGAVRLKVGDRLGLRDPKKMAFLWVTEFPMFEWDEEGQRPTATHH
                     PFTSPVMEDLPILESDPLNVRARAYDVILNGTELGGGSIRIHSSEVQKRVFSAMRISD
                     ESARVKFGFLLDAFEFGPPPHGGIALGLDRMIMLMCGAPSIRDVIAFPKTTSASDLMT
                     DSPSDVDEAQLRDLHIAIKH"
     gene            complement(4229..5932)
                     /locus_tag="PKH54_05445"
     CDS             complement(4229..5932)
                     /locus_tag="PKH54_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99368.1"
                     /translation="MRAMTCVLLALAVSLGCGTGPEGTDAFDHGSPGDDLPDVQQEIP
                     GDTTEDANPDTTLDSAIDGNWTDSADDDGGILNDTAADDGDDNGDIGDTGPTDFVRPP
                     RGEPVDSVEIGRITDLYLEILEKTRYFDVMNERVHGWPRDDAGGRYWYGTWWSGITMT
                     KTDGMVTFLHSPDGADNNGMRGAPMLVGTCFASRLWGKAEHVALARKLVRGFNSWAYV
                     FRSPTHPDWEAKQVLSRAAYPESVETHDDGLHRFIDYSKNRPGEDNGACEYVEVTDNP
                     YWGHIFAKNKRSKDDIGHMFIALALMNQCIVGQDETDPEVMGLMDDIEESMEIYRNWA
                     AQVDEDGWKIATVDKAGTIYYPDEDLAYLWLEGANIECEAAVTLRLFGGGNPDDLACG
                     NGINPYFGEEDALKNDFHQIQRSFHEAAIMHSWVAGRGDVATELLDGLAWRLDTRLDE
                     LESLEPPEDPHLGDLGELVLMAGNSGLPLTWREVRFLHARIEESYNALVGTQDQAALK
                     ARFDVFSPDVADGTYPFDPGDAGMAWRYLGAILGVCASPFWSSGSMQVLDCEKIRQAT
                     M"
     gene            6060..>6601
                     /locus_tag="PKH54_05450"
     CDS             6060..>6601
                     /locus_tag="PKH54_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99369.1"
                     /translation="MKEGLRHPPEGSPGLLEDELRSRVEWLVRLRWIAAAMVLAGTGS
                     AYLIPGIRLPWLALSACGTAILAYNTVFMLLARRLAKQPKVGARWFSMLASAQFITDW
                     IALTILVHLTGGVGSPILFFFVFHAILASILLPPRAAWFHAAGGVLLVGGLFALEYLG
                     AVDQMPVAGFFAIPAREPMVA"
BASE COUNT         1196 a         1902 c         2077 g         1426 t
ORIGIN      
        1 agacttccgt caacatcgtc cgcgaggccg gtgaggaaga gaagctgttc ggatccgtga
       61 cgaatcgcga catcgccgag gccctggccg ccgaaggcat cgttgtcgat cacagggtca
      121 ttctgcttga aaacccgatt aagacgcttg gatccttcaa ggtcgaagtc aagctgacca
      181 cggaagtcag agccgaggtc aaggtccggg ttgttgcgaa gtgacgattc cacgactgtt
      241 tgaatcgggg cgggaactcc gccccggttc ttcattcacc cccgaaatcg atgtaatgca
      301 ttatctgcgg cgcgtgcttc gcctgaagcc cggcgatggc gtcctggtgc ttgatggcgg
      361 gccggttgcc tggctcggga cgcttgcggg aagcgatgac ggtcttggaa tcgcgatttc
      421 ggggccggcc gaaggcgtgc gtgtcgtccc ggacggtcgg cggatctgtg cgcttgttcc
      481 cgcgctgaaa ggcgatcggc acgagttcgc gatccagaag gccaccgaac ttggcgtgac
      541 cgagttgaga atctatttcg ccgacaggtc gattccgcgc ccgtccgcgc gtgaaagcgc
      601 caagctcgcc cgctggatga aggttgccga ggaggcgtgc cgtcaatgtg acagggcctc
      661 ggttcctctg gtcgggatct atgacgatct cgggtgcgcg acaggtgaac tggatatcta
      721 tgccgaccgg tacctgctgt ccgaaatcgg aacccccgag ccgcttgcca gaattgcgac
      781 cggaacgcgt ccggcagtag tcgccatcgg tcccgaaggc tcgtttacgg aggcggaacg
      841 cggtatgctt cttcaggccg gtttcgttcc ggccggcctg gggccaaggg ttctgaaggc
      901 ggagactgcg gttatcgcgg ctgtaatggc cgtgcaggtt gccggcgggg atatcggatg
      961 agcaagagtg agccgggtgg tcatgacgag ttcgtggttc gcgtcgcggc atccacgctt
     1021 cgcgtcctgg ccggcctgat agatcttgcc gtgccggccg gcctgacagt gcttgcctgc
     1081 tttctttccg agactcccga tctgtcccgt cttcccccca gatactggaa ttatcttgat
     1141 tacaccgttg acgtgttcaa cagccagcca tcggttttca ttgttcccgc gctctatttt
     1201 gttgccctgt atgtccttgg aacggttgtc ttcgtttcga ccattggaaa cacgccgttt
     1261 tccaggctgg tcggcataag ggtgaccgac aggcgaggtc ggggcatcgg tccgatccgc
     1321 tcatttttct ggaccgtttg cgggctgctg ctgggtgttg tggcgtttgt cgggccgctg
     1381 tggacaatcg tcgatccgaa aaggcgcatg ctgcatgata tacttgccgg cgtcgtggtc
     1441 gtattcggca ggccggtggt caccatggta actgccgatc ctgacgaggc gcctgttgac
     1501 gacattttcc cggcaccaga agaggtgaag ggcgatgtga atggcaggtc tgccggaggc
     1561 agcgccccct ggcacgcaga gggaggtaac tggtgatttc ctttcgtcgt ttcaatctgc
     1621 tggttttttc ggtcttagcg accttgacgt tttccggcgc ggttgcgtcg gcggcaccga
     1681 aggattcggc catgcccgcg agaccgacga ttgttgacta ttcgggaaag atggtcgatg
     1741 aagccgggcg tcccgtttcg gggatttatc caattgaatt caaactgtac tccccgggat
     1801 cgaagaagcc ggttttcagc gatcggatgt gggtcgccgt cgtggatggc gcctacacgg
     1861 tcgggatcgg ttcccagaaa ctgcttcccc gcggccagga tttcacgaag ctgatgcttg
     1921 gcgtcgagat ttccggcgtc gaactttcga ggcaggtctt catggacaag gacaccgcca
     1981 ggaaggttca gcaggacatg gagaagaagg ccgccgaggt ttccgatacg aagctttcgg
     2041 tcgattccgc ccgcgccggc tccccggcat cgaataccgg ctcggtcaag tacgcggact
     2101 ccgcgggata cgcggtgtcg tcggatcatg cgaagaacgc ggatcgcatc cagaatctga
     2161 ccgtcgatga cctggtgcgg aaggttgttg aggaaggtgg cggcgggtcg tccggcggaa
     2221 gcgtgacgat tggccggaca aggcgctatg gtgacagggt tggcggtccc ggcggcgaca
     2281 tcgagtacaa cgaggtctgt ccgaagggat acgtcatggt tggagtcagg ggaggggccg
     2341 ggaagttcct ggactcgata cagatcgtct gtgctccgct tggggcagaa taacagcgct
     2401 attgcggttt tggaggtcgt ttcagatgga cggcgtacgt acacataatt gcggggaatt
     2461 gagggaatcc gacatcggcg cccaggtcat gctgcagggg tgggccgcca gcgttcgcga
     2521 ccacgggggc tgcgccttca taaacctgcg ggatcgcttc ggcacgacgc agatcaagtt
     2581 cgaccaggcc cttaatccgg cggcgtttga caaggccgtg ggcgtcagat cggagttcgt
     2641 tatccaggtc acgggcacgg ttgtcggcag gggcgccaat gccaacctta agattccgac
     2701 cggcgggatt gaggtcgagg ccgggtcaat cgagatcctg agctccgcga agccgctgcc
     2761 gttccagatt gcggacgatg tcgatgcgca ggaagtgacg cgcctgaagt acaggtatct
     2821 tgatcttcgc cgcaatcagt tgaattccaa catcgtgatg cggtcaaagg tctgcaagat
     2881 ggttcgcgac tatctggaaa ccagggactt ccttgaaatc gagacaccca tcctgatgaa
     2941 gagcacgcct gaaggggcgc gtgatttcct cgtgccatca agggttcatc ccggccagtt
     3001 ttacgcgctt ccgcagtcgc cccagacctt caagcaactc ctgatggttt ccgggttcga
     3061 ccggtacttc cagattgcgc gctgcttccg cgacgaggac ctgcgcgcgg atcgccagcc
     3121 tgaatttacc cagatcgaca ttgaaatgtc attcgcggtt ccagagctga tatatgaaat
     3181 tgtcgaaggc atgctgaagc atgtctggaa gcacgcgctg ggaatcgaca tccagacccc
     3241 gttcctgcgc atgccgtact ccgaggcgat ggagaagtac ggttgcgaca aaccggacat
     3301 gcgcttcggt atggaaatgg tcacgataac cgatgaagtc gcgggcagcg ggttcaaggt
     3361 gttttccgac accgcggccg ccggcggcgt tgtcacggca atcaaggtgc cggacgcgga
     3421 gaaatggtcc aggaaggata tcgatgcact gacccagacg gccacggaca atggcgcaaa
     3481 gggtcttgcg tggttcaaga tcggtgaaag cgaatggtcg ggatcggcag ccaagttcct
     3541 ttccgatggc gacaagagcg cgatcgccac gaagatgcag gcaggccctg gcgacctgct
     3601 cctggtcgtc gcggatcgcc cgaacaaggc ccgccaggcc atgggggcgg tcagactgaa
     3661 ggtcggcgac aggctcggcc tgcgcgatcc gaagaagatg gctttcctgt gggtcacgga
     3721 gttcccgatg ttcgaatggg acgaggaagg ccagcgcccg acggcgacgc atcacccgtt
     3781 cacttcgccg gtcatggaag accttccgat tcttgaaagc gacccgctta atgtgcgggc
     3841 acgcgcctat gacgtcattc ttaacggaac cgagcttggc ggcggttcaa tccgtattca
     3901 cagcagcgaa gtccagaagc gcgtgttttc ggcgatgagg atttcggacg agtcggcgcg
     3961 tgtcaagttc ggcttcctgc tggatgcgtt tgaattcggt ccgccacccc acggcggcat
     4021 cgccctcggg cttgatcgaa tgataatgct gatgtgcggc gcgccttcga ttcgcgacgt
     4081 gattgcattc ccgaagacga caagcgcgtc cgatctgatg accgactcgc cgtccgacgt
     4141 tgacgaagcc cagcttcgcg atctccacat cgccatcaag cactgacatc gccttcattc
     4201 gtggggcggc cggaaagtgg tgcgctgatc acatcgtcgc ctggcggatc ttttcgcagt
     4261 caagcacctg catcgagccg ctgctccaga acggggacgc gcagacgcca aggattgcgc
     4321 caaggtacct ccatgccatg ccggcatcac ccggatcgaa tggataggtg ccgtcggcga
     4381 cgtccggcga gaacacgtcg aagcgtgcct tcagggcagc ctgatcctgc gtgccgacca
     4441 gggcgttgta cgactcctcg atgcgggcgt gcaggaaacg gacctcgcgc caggtaagcg
     4501 gaagaccact gttaccggcc atcagcacca gttcccccag atcgccaagg tggggatcct
     4561 ccggcggttc caggctttcc agttcgtcca gccttgtgtc caggcgccat gcgagaccgt
     4621 cgagcagttc ggtggcgaca tcgccgcgtc cggcaaccca ggaatgcatg atggcggcct
     4681 cgtggaagct tcgctggatc tggtggaagt cattcttcag cgcgtcttcc tcgccgaagt
     4741 atgggttgat tccgttgccg caggccagat cgtcggggtt gccgcccccg aaaaggcgca
     4801 gtgttaccgc ggcttcgcat tcgatgttag cgccttcaag ccacaggtat gccagatcct
     4861 cgtccgggta gtagatggtt ccggccttgt caactgtggc gatcttccag ccgtcctcat
     4921 cgacctgggc tgcccagttg cgatagattt ccatcgattc ctcgatatcg tccatcaggc
     4981 ccattacctc gggatccgtt tcgtcctgtc cgacaatgca ctggttcatg agggcgagtg
     5041 caatgaacat gtgcccgatg tcgtccttgg atcgcttgtt cttcgcgaat atgtggcccc
     5101 agtaagggtt gtcggttacc tcgacatact cgcaggcccc gttgtcctcg cccggcctgt
     5161 tctttgaata gtcgatgaag cggtgcaatc cgtcgtcgtg tgtttcgacc gactcgggat
     5221 aggccgcccg cgacagtacc tgctttgcct cccagtccgg atgggtcggg ctgcggaaga
     5281 cgtacgccca tgagttgaat ccccgtacca gcttgcgcgc cagggcgacg tgttccgcct
     5341 tgccccacag ccttgacgcg aaacatgtcc caacgagcat cggtgcgccc cgcatgccgt
     5401 tgttgtcggc accgtcgggc gaatgaagga acgtcaccat gccgtcggtc ttcgtcatcg
     5461 taattccgga ccaccaggtt ccataccagt aacggccacc ggcatcgtcc cttggccatc
     5521 cgtggacacg ttcgttcatt acgtcgaaat agcgtgtctt ttcaaggatt tccaggtaca
     5581 gatcggtgat tcgtccgatc tcgaccgaat cgaccggttc gccccggggc ggcctgacga
     5641 aatcggttgg cccggtgtcg ccgatatccc cgttgtcgtc gccgtcgtcg gccgcggtgt
     5701 cattcaatat tccgccgtcg tcgtcggccg agtccgtcca gttgccatca atggccgaat
     5761 caagggtggt gtccggattg gcatcctcgg tggtgtcgcc cgggatttcc tgctgaacgt
     5821 ccggcagatc atcgcccgga ctgccgtggt cgaaagcatc cgtaccttcg ggaccggtcc
     5881 cgcagcccag tgagacggca agagccagaa gaacacatgt cattgcacgc attgcgggct
     5941 gtacctccat catgccggcc ctgcatgggt cggacgcttg aatcatatca acaaccggcc
     6001 ggttgcggtt acaggtcttt cgatacatca ttgtccggtc gggccatgga gaacggagtt
     6061 tgaaggaagg tttgaggcat ccccctgaag gctcaccggg gcttctcgag gacgaacttc
     6121 gttcccgggt cgaatggctg gtacgcctgc gctggattgc ggcggcgatg gttcttgccg
     6181 gaaccggttc cgcttactta attccgggaa tcaggttgcc ctggctggca ctttcggcgt
     6241 gcggcaccgc catccttgcc tacaacacgg tcttcatgct gcttgcgcgg cgcctggcaa
     6301 agcagccgaa ggtcggggcg cgctggtttt ccatgctggc cagcgcgcaa ttcatcaccg
     6361 actggattgc gcttaccatc ctcgtgcatc ttaccggggg agtcggaagc ccgattcttt
     6421 tcttctttgt tttccacgcc atcctcgcaa gcattctgct gccgccccgt gccgcctggt
     6481 tccatgcggc aggtggcgtc ctgcttgtcg gggggctgtt tgcccttgag tacctgggcg
     6541 cggtcgatca gatgccggtg gcaggttttt tcgccatacc ggcccgcgag ccgatggtcg
     6601 c
//
LOCUS       DAOPPA010000132         6589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12818_length_6589_cov_4.451944, whole genome shotgun sequence.
ACCESSION   DAOPPA010000132 DAOPPA010000000
VERSION     DAOPPA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12818_length_6589_cov_4.451944"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..449)
                     /locus_tag="PKH54_05455"
     CDS             complement(219..449)
                     /locus_tag="PKH54_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC99370.1"
                     /translation="MRELVTYIAKALVDLPDDVSVTEVEGEQTSVIELRVAKEDMGKV
                     IGKQGRTAKAIRTVVNAAATKMNKRAVLEIIE"
     gene            complement(484..723)
                     /gene="rpsP"
                     /locus_tag="PKH54_05460"
     CDS             complement(484..723)
                     /gene="rpsP"
                     /locus_tag="PKH54_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOC99371.1"
                     /translation="MVKIRLARFGAKKRPFYRIVAADSHGKRDGRYIELLGTYDPLKD
                     PPEVRLNSERILYWKSVGAQLSDTVASILKANKIA"
     gene            934..1488
                     /locus_tag="PKH54_05465"
     CDS             934..1488
                     /locus_tag="PKH54_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOC99372.1"
                     /translation="MAHKRPRPGCIPVGEITENGLDWTMQIPDDVVAGLLTLQFLPSG
                     QPLAIDVRLLLAGKNVIAQGRVYGKLRAVCGRCLAERDFNLERRFRHVFIEGRDPAGD
                     KPEEVLGETDDLDCTFFDDEQVDILSLACDEIGLALPLNPLCRPDCRGLCPMCGADRN
                     LDPCGCVIDDVDPRWTALKDLKLD"
     gene            1565..1756
                     /gene="rpmF"
                     /locus_tag="PKH54_05470"
     CDS             1565..1756
                     /gene="rpmF"
                     /locus_tag="PKH54_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOC99373.1"
                     /translation="MAVPKKKKSRSKRDSRRANHDRLTLRSLVKCSRCGAVRLPHRAC
                     RECGFYRDRIVVATEPTGA"
     gene            1761..2204
                     /gene="rpiB"
                     /locus_tag="PKH54_05475"
     CDS             1761..2204
                     /gene="rpiB"
                     /locus_tag="PKH54_05475"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOC99374.1"
                     /translation="MKIGIGSDHAGCGLKARIVQHLAAAGHQVTDVGTDSPDRSVDYP
                     VYARKVAQSVASGDLELGILVCGTGIGMSIAANKIDGIRAALIHDHVTARMARMHNNA
                     NVICMGARLLAAEYAIDLVDQFLSCPFEQRHQRRLDLITDIERGG"
     gene            2209..2694
                     /gene="nrdR"
                     /locus_tag="PKH54_05480"
     CDS             2209..2694
                     /gene="nrdR"
                     /locus_tag="PKH54_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOC99375.1"
                     /translation="MKCPICGNPDSRVIDSREAGTGDAIRRRRECESCHRRFTTYERA
                     EEFIPRVIKKDGRREEFSRSKLMAGLSRACQKTRVSSVELEAFIDGLLARIQDRMATE
                     VESCWIGGEVMDFLREKDPVAYVRFASVYRRFTDIEAFEDEIRLLLRQPERRDSPEAE
                     R"
     gene            2691..3827
                     /gene="ribD"
                     /locus_tag="PKH54_05485"
     CDS             2691..3827
                     /gene="ribD"
                     /locus_tag="PKH54_05485"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOC99376.1"
                     /translation="MIRESTGIEDPRFSAFDLAMMRRVLAVAGRGRGLVSPNPMVGAV
                     VVRDGQVVATGYHHRHGSSHAEVDALSRIGFDAQGCTLYVNLEPCCHQGMTPPCTSSV
                     ISSGVSRVVIGTMDPNPLVSGRGIASLRQAGLQVDVGLLESACRELNRGFFKWITTGL
                     PFVTLKMACTLDGRTADRHGRSRWITGEPARRDVHRLRADSDCVMVGGNTASHDDPLL
                     LPVMVRARRNPVRAVVSGSGGIEPGSRLAMSVGDGPVFLFTSDRAARERVDALAGSGI
                     QVISVPSTDDGLDLPAVLRELGARRITSVMVEGGSTLAASLLRASLVDRLVVYYAPIL
                     VGDPSAPGLCSDMGIHDIGRSLRFKLIDSVRVGCDVRMRFDPEG"
     gene            3885..4529
                     /locus_tag="PKH54_05490"
     CDS             3885..4529
                     /locus_tag="PKH54_05490"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOC99377.1"
                     /translation="MFTGIIESRGTIAQVRSRGQGLELHVLAQLPGGPLVPGESIAVS
                     GPCLTVEKVVAGGFVVFASAETTARTTIGSLARGDRVNLERAMAASGRFGGHMVTGHI
                     DGIGRLVQSQERGEATGLEFQVGSEISRFIVARGSISIDGISLTVTEADAGRFKVMVI
                     PHTLSNTTLDGIKPGASVNVEVDIVARYIFAFTRPSDGGITAARLAELGWDVGD"
     gene            complement(4530..5540)
                     /locus_tag="PKH54_05495"
     CDS             complement(4530..5540)
                     /locus_tag="PKH54_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99378.1"
                     /translation="MPIFQQIQARNSRHRMEAAILATCAIALATSCRGRDPEKTMVKV
                     GLKSVQVESDFSVRARFALAVPQDTGIRVHLFDGPGFDCSSTLDESAVAELYIPGSPG
                     QPMGVKFHQNDRPSWATSLNPGKDPPFSDVRRHDDRVTGCIDADIDLRSAGNTASIHG
                     CFSAALCPARGQSGLAATKGSSSVHISMTTPQGTWTTRPGAAIAFAAEDRLMVFFQDT
                     AILNCENYRLVPKQGLIEFATTPGRDRFLTGDLVKVAPVDPKTGWSFHYGPDLFRANV
                     ATAVVNRESNRVTGTVDVTFWGLDRQTLSANFPDGTGFDHHKEVTGHFRGDFDAPLCP
                     EF"
     gene            complement(5615..6511)
                     /locus_tag="PKH54_05500"
     CDS             complement(5615..6511)
                     /locus_tag="PKH54_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOC99379.1"
                     /translation="MKVGFVSRDGRIIPVMPTACGLQDLSRFAEILLPGFNFPVGIAL
                     LRALSGNEPRTMTVLQAARSAAAREDGCMTVDPEGIRWLPPVPDTRQIIAVGLNYRSH
                     CIEQNIPIPAEPKFFAKLVSSLTGHLEPIRLWEISSRIDYEGELGIVIGRQTRAVTLS
                     GALACIGGYTIVNDVSARDLQKGDGQWTRAKGLDTFCPMGPFLTTPDEVPDPDNLRIL
                     TTVNDVIRQDCSTSDMIFGVARLVSTISNFITLMPGDVISTGTPCGVGLYSDPPCFLK
                     AGDVVNICIEGLGTLSNVAMNP"
BASE COUNT         1198 a         1896 c         2075 g         1420 t
ORIGIN      
        1 ccgtcgattt tccgtcggga aggcgcagcg tgatttccat cccctcgaac gggagttcgg
       61 acgccaggtt gaacgggacg tagtttacct cgcccccaag tccgtgcgcc gacgcgattt
      121 ttcccagctc gattggggat ttcatgttca tgaagtcttg gttctgcgaa gggcattccc
      181 gtcatgcgga aatgccggga aagtaccggc agggcccgtc actcgatgat ttcaagcacg
      241 gcgcgcttgt tcatcttcgt ggcggcggcg ttcaccaccg tcctgatggc cttggcggtg
      301 cggccctgct taccgataac cttgcccatg tcttccttgg caaccctcag ttcaatgacc
      361 gaggtctgtt cgccttcgac ttcggtaacc gaaacatcgt cgggcaggtc aacaagagcc
      421 ttcgcgatat aggtaacgag ttcccgcatg gcttcggcca taggtacctc ccgaaagggc
      481 gccctaggcg attttgttag ccttcaggat gctggcgacc gtgtcggaca actgggcgcc
      541 gacagacttc cagtaaagga tccgttcgct gttgagcctg acttcgggcg gatccttgag
      601 aggatcgtat gtgcccagga gttcgatgta gcggccgtcg cgctttccgt gcgagtccgc
      661 ggcaacgatt ctgtagaaag gacgcttctt cgccccaaac cttgccaatc tgatcttaac
      721 catgaaacag acgcctccgc gcttgataaa tggacaaccc aaagccgcgt tattttacca
      781 ccggtttggg ctttggcaag tcccttagtc ctgttcgtat tccacgatga aatcggccat
      841 ggtgaaaatt attgacggct tgacttaata ggggtttgcg cctagtatcg gtggccccgt
      901 aaaacggggg ctctatccgg gggcattgcg ggaatggcgc ataaaaggcc gagacctggt
      961 tgcattccgg tcggcgagat cactgaaaac ggtcttgact ggacgatgca gataccggac
     1021 gacgttgtcg ccgggctgct gaccttgcag tttctgccgt cggggcagcc gctggccatt
     1081 gatgtcaggc tgctcctggc cggaaagaac gtcatcgccc agggaagggt ttacgggaag
     1141 ctccgggccg tttgcgggcg ctgtctggcg gaaagggatt tcaacctgga acgccggttc
     1201 aggcatgtgt tcattgaagg aagggatccc gcgggcgaca agcccgaaga ggttcttggc
     1261 gagacggacg atttggactg cacgttcttt gacgacgagc aggtcgatat cctttcgctg
     1321 gcgtgcgacg agattggtct tgcgttgccg ctgaacccgt tgtgccggcc ggactgcagg
     1381 gggctttgcc cgatgtgcgg cgccgacagg aacctggacc cgtgcggctg cgtgattgac
     1441 gatgtcgatc cgcgctggac cgctttgaag gatttgaagc tggactgatg ccagccgggt
     1501 gttgcccgga caaggcgctt ccggttggct tgttactgtt tgaatcagtt cacggaggct
     1561 gatcatggct gttccgaaga aaaagaagtc ccgttcgaag cgtgattcgc gtcgcgcgaa
     1621 tcatgaccgc ctgacgttgc gttcgctggt caagtgttcg cgttgcggcg ccgtaaggct
     1681 gccccacagg gcctgccgcg agtgcggttt ttaccgtgat cgcatcgtgg ttgccactga
     1741 accgaccgga gcctgacccg atgaaaatcg gcattggctc agatcatgcc ggttgcggat
     1801 tgaaggcgcg catcgttcaa caccttgccg ccgccgggca tcaggtaacg gacgtcggaa
     1861 ccgattctcc cgacagatcc gtcgattatc ccgtttatgc ccgcaaggtc gcccagtcgg
     1921 ttgcttccgg cgatctcgaa ctggggatcc tggtatgcgg aaccggcatc gggatgtcca
     1981 ttgccgcgaa caagattgac gggatacgag ccgcgctgat tcatgatcac gtcaccgccc
     2041 gcatggcccg gatgcacaac aacgccaatg tcatctgcat gggcgccagg ctcctggccg
     2101 ccgagtatgc gatcgatctg gttgaccagt tcctgtcctg cccgtttgag cagcgccacc
     2161 agcgccgtct tgacctgatt accgatatcg aacgcggggg ctgaagccat gaagtgcccg
     2221 atctgcggca atcccgacag ccgggtaatc gattcccgcg aggccggtac cggcgacgcc
     2281 atccgtcgtc gtcgtgaatg cgagtcctgc caccgccgtt tcaccactta tgaacgcgcc
     2341 gaggaattca ttccaagggt tatcaagaag gatggccggc gtgaggaatt cagtcgctcg
     2401 aagctgatgg ccggtctatc ccgcgcgtgt cagaagacgc gtgtttcgag cgtcgaactc
     2461 gaggccttca tcgacgggct gctggccagg atccaggaca ggatggcgac cgaggtcgag
     2521 agttgctgga ttggtgggga agtcatggat ttcctgcgtg aaaaggatcc ggtcgcatac
     2581 gtcaggttcg caagcgtcta tcggcgtttc accgatattg aggcgtttga ggatgagatc
     2641 cgcctgttgc tgaggcagcc cgaaaggcgc gattccccgg aggccgaacg ttgattcgcg
     2701 aatcgacagg aatcgaggat ccccgttttt ccgctttcga tctggccatg atgcgccgag
     2761 tccttgcggt tgccgggcgc gggcgcggcc tggtcagtcc gaatcccatg gtcggtgccg
     2821 tcgtcgtccg tgacggccag gtggtggcga ccggttatca tcatcgtcac ggctcgtcgc
     2881 atgccgaggt tgatgccctt tcccgcatcg ggttcgacgc ccaaggctgc acgctttatg
     2941 tgaatcttga gccctgctgc catcagggaa tgacaccgcc ttgcaccagt tcggtcattt
     3001 cgtccggtgt cagccgggtg gtcatcggca ccatggatcc caatcccctg gtgtcggggc
     3061 gtggcattgc aagtcttcga caggccgggc ttcaagtcga tgtggggctg cttgaatcag
     3121 cctgtcgcga gcttaatcgt ggtttcttca agtggatcac cacggggctt ccgttcgtga
     3181 cccttaagat ggcgtgcacg cttgatggaa gaaccgccga caggcacggt cggtcgcgat
     3241 ggattaccgg ggaacccgcc aggcgggacg tgcatcgtct tcgcgccgac agtgactgtg
     3301 taatggtcgg cggcaacacg gcctcccatg atgacccgct tcttcttccg gtgatggttc
     3361 gggcccgcag gaatccggtt cgcgccgtcg taagcggatc cggcgggatc gagcccggat
     3421 ccaggctggc catgtcggtc ggtgacggtc cggtgtttct tttcacgtcg gatcgggccg
     3481 cgcgcgaacg ggtggatgcg cttgcgggat cgggcatcca ggtcatttcc gtcccttcga
     3541 cggacgatgg ccttgacctg ccggctgttc tgcgggaact cggggcgcgc cgaatcacat
     3601 cggttatggt cgaaggcggc tccacgctgg ccgcgtccct gcttcgagct tcgctggtcg
     3661 atcggctggt cgtctattac gcgccgatcc tggtggggga tccgtcggcc ccgggccttt
     3721 gctcggacat gggaatccac gatatcggca ggtcgcttcg tttcaaactg atcgattcgg
     3781 ttcgcgtcgg ctgcgacgtc cgcatgcgtt ttgatcctga aggttgaggg cctttttcag
     3841 gtacggggat ataatgtccc ggtacgtgcc atctggaggc cgccttgttt accggaatca
     3901 tcgaaagtcg gggcaccatc gcccaggtgc gttcccgcgg ccagggcctt gagcttcacg
     3961 ttctggcgca gttgccgggc gggccgctcg ttcccggtga aagtatcgcg gtttcagggc
     4021 cttgtcttac cgtcgaaaag gttgttgcgg gcggattcgt ggttttcgcg tcggccgaaa
     4081 cgacagccag gacgacgatc gggtcgctgg cccgtggcga cagggtgaac cttgaaagag
     4141 ccatggccgc ttcagggcgg ttcggcggcc atatggtcac cggtcacatc gacggcatcg
     4201 gcaggcttgt tcagtcgcag gagcgtggcg aggcgaccgg tcttgaattc caggttggct
     4261 cggaaatctc caggttcatc gttgcccggg gatccatatc gattgacggg atttccctta
     4321 ctgtcacgga agccgatgca ggtcggttca aggtgatggt cattccgcat acgctttcga
     4381 acaccacgct tgatggcatc aagcctggcg cttctgtaaa cgtcgaggtc gatatcgtgg
     4441 cccggtacat cttcgccttc accaggcctt ccgacggcgg gatcaccgcc gccaggcttg
     4501 ccgaactggg ttgggatgtc ggggactgat cagaattcag ggcacagggg cgcgtcgaag
     4561 tcccccctga agtggcccgt cacttccttg tgatgatcaa atcctgtgcc gtccggaaag
     4621 tttgcggaca gggtctgccg gtcaagtccc cagaaagtca catccaccgt tcccgtcacg
     4681 cggttgcttt cgcgattgac cacggccgtc gcgacgttcg ccctgaacag atccggcccg
     4741 taatggaagc tccagccggt tttcgggtca accggggcca ccttgaccag atccccggtc
     4801 aagaagcgat cccggcctgg cgttgttgca aattcaatca ggccctgctt cggcaccagg
     4861 cggtagtttt cgcagttaag gatcgcggtg tcctggaaaa agaccatcag ccgatcctcg
     4921 gcggcgaagg cgattgccgc accgggacgg gtcgtccacg taccctgcgg cgtggtcatt
     4981 gaaatgtgaa cgctggaact gcctttcgtg gccgccagtc cagattgccc gcgcgccgga
     5041 cagagtgccg ccgagaagca tccatggatc gatgcggtat tgccggcgga acgaaggtcg
     5101 atgtcggcat cgatgcatcc ggtgacgcgg tcgtcatgtc gcctgacgtc ggaaaaaggc
     5161 ggatcctttc cagggttcag gctggtcgcc caggaaggcc tgtcgttctg gtggaacttg
     5221 acgcccatcg gctggcctgg tgaacccggg atgtaaagct cggcgaccgc cgattcgtca
     5281 agggtcgatg agcaatcgaa tcccgggccg tcgaagaggt gaaccctgat ccccgtgtcc
     5341 tgcggcacag ccagggcgaa ccgtgccctg acggagaagt cggattcgac ctgaaccgac
     5401 ttcaggccga ctttcaccat ggtcttttcg ggatccctgc cacggcagga cgtcgccaga
     5461 gcgattgcgc aagtggcaag gatggccgct tccatgcggt gtcgggaatt ccgggcctgg
     5521 atttgctgga aaatcggcat tggacgtcca cgtggcggcc cgccgccaag gttatcactt
     5581 ccggccctgg ccggaaaccc gggccggtcc ggggtcaggg attcatcgcg acgtttgaaa
     5641 gtgtgccaag tccctcgatg cagatgttga ccacgtcccc ggccttcagg aagcagggcg
     5701 gatccgagta aagcccgacc ccgcagggcg tgccggtcga aatgacgtca cctggcatca
     5761 gggtgatgaa gttgcttatg gtcgaaacca ggcgggccac gccgaagatc atgtcggacg
     5821 tcgaacagtc ctgccggatc acgtcgttaa cggttgtcag gattctcagg ttgtcagggt
     5881 ccggaacctc gtcaggggtc gtcaggaacg gacccatcgg acagaaggtg tcaaggccct
     5941 ttgcccttgt ccactgcccg tcgcccttct gcagatcgcg tgcggaaaca tcgttgacga
     6001 tggtatagcc gccgatgcat gcaagtgccc cggaaagcgt aaccgcccgc gtttgccggc
     6061 cgatcacgat gcccagctca ccttcgtaat cgattctgga gctgatttcc cagagcctta
     6121 tcggttcaag atgtccggtg agtgaagaaa ccagcttagc gaagaatttc ggctcggccg
     6181 ggatgggaat gttctgttca atgcagtgcg atcggtaatt caggccgacc gcgatgatct
     6241 ggcgcgtgtc gggaaccggc ggcagccacc tgatgccctc gggatcgacg gtcatgcagc
     6301 catcttccct tgccgcggcg cttcttgcgg cctgaagcac ggtcatcgtg cgcggctcgt
     6361 tacccgacag cgcccgcaga agggcgatcc caaccggaaa attgaaaccg ggcagaagaa
     6421 tttcggcgaa cctggacaga tcctgcaggc cgcaggcggt cggcatcacc gggatgatcc
     6481 tgccgtcacg tgacacgaat ccaaccttca tggcgcctcc atgtcgggca atcgcttcga
     6541 tgatggtatc agaaggcagc ttcttgaaat atagttgtcg tgttcagtc
//
LOCUS       DAOPPA010000133         6585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12825_length_6585_cov_3.896172, whole genome shotgun sequence.
ACCESSION   DAOPPA010000133 DAOPPA010000000
VERSION     DAOPPA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_6585_cov_3.896172"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1570)
                     /locus_tag="PKH54_05505"
     CDS             complement(56..1570)
                     /locus_tag="PKH54_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC99380.1"
                     /translation="MKADKWFVSGDLNAFFALFVDNVVNLVMLTAILAGQFGMPQDFI
                     LTRMIPGTAIGVMFGDLAYTWMACRRGRRMTAMPLGLDTPSTIGMTIAVIGPVFLESG
                     NAMTAWSVGMATLFIMGLFKVVMSFAGDWVQRIVPLPALLGPLAGIGLALLGLIPVMR
                     IFSAPVAGLFATGVILYSIVAGFRLPMRIPGALLAVVAGLIIYWVMAIAGLSPAPVTH
                     SATIGLNWPGIDLSALTSGMAGAIRYLPVSIPFGLLTIVGGINTTASARAAGDDYRTR
                     DILLVESVATLIAGALGGVAQSTPYIGHPAYKAMGARAGYTFLTGIVIGLAGIFGVLG
                     VIIDFVPEVVVAPVLLFVGLAITEQAFSASPRRHMMAVAFSIIPCLAYVITIYTGQLL
                     GAGARPDPSLAAQLGILAILGNGFIVTAILWGSALVFLIDRKPLAVLLTFVTLGVFSL
                     FGVIHSVAPGGGLYLPRLPEAARSFELAAAYVLTGAALAAMLRWFPQESGQAGQ"
     gene            complement(1674..2114)
                     /gene="ssb"
                     /locus_tag="PKH54_05510"
     CDS             complement(1674..2114)
                     /gene="ssb"
                     /locus_tag="PKH54_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC99381.1"
                     /translation="MASLNRVLIIGNLGSAPEIRTTGGGQTVATLSVATNEVWNDKAG
                     QKQERTEWHKVIVWGRQAEFCGQYLQKGSTVFVEGRIQTRSWDDKDGQKRYTTEIVAS
                     NVQSIGGRGGADNAHQSYGEPPVAEPVGMPVTTPSAINDVDVPF"
     gene            complement(2196..3362)
                     /locus_tag="PKH54_05515"
     CDS             complement(2196..3362)
                     /locus_tag="PKH54_05515"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOC99382.1"
                     /translation="MSIIVQKYGGTSVADLERIKKVACHVVETRRAGHDVVVVVSAMS
                     GKTDELMRMATDINPEPSMRELDMLLTVGERITMALLSIAIQAMGQDAISLTGSQCGI
                     MTNHSHSHARIVEVRPFRVLDELEAGKIVIVAGFQGTSYKREITTLGRGGSDTTAVAL
                     AAALNAERCEIYSDVLFVCSADPRVVINARQFQELDYEQMLSLSRHGAKVLNADAVEF
                     ARRAGIAIYTRSSMDPLKEGTVVRVNPDAPPRSVVGVTARKDVVALRFSSDGGVSDQA
                     LSMLAAGGLTPCFVCAAGGGGTWMVFSRTDNLALDGFLTASLPEGATLVKDIGAVTVV
                     GDVLADPSGPAAISHVRSIGHLRGMMLAPAAMTLLVDADKVDSVTRELHEVFFA"
     gene            3552..5273
                     /gene="argS"
                     /locus_tag="PKH54_05520"
     CDS             3552..5273
                     /gene="argS"
                     /locus_tag="PKH54_05520"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOC99383.1"
                     /translation="MNRNFEEIAAGTLAEALDKLKAAGGVPDDFEPKIVIERPKNPDF
                     GDFGCALPMLMCKATRTAPAICGRNLMDKIQAPPGIFDEISFAPPGYINFRVSPTAWF
                     EGLQAVCDIPDVGHGQKVLLEFVSANPTGPLHAGHARGAVLGDIVARLMKVAGYEVET
                     EYLLNDVGNQMAMLGLSIFYRGRQALGLASEVDFPENGYRGDYIREIAARFVSGQEGR
                     DFCAASYPEGAGRDDNPAVRFAAGLLTEEIRKTLDCLAIRFDHWFSERSLHDSGRVAF
                     LVNRLLESGAAYRTDDGAILFSMEGDEDGEDRVVVRGNGVPTYFAADIAYHDEKARRG
                     YDRLINIWGADHHGYIPRVRAAMGALGHSPSMLDVILVQMVTLTRNGEVVPMGKRLGR
                     FVTLQDLVDEVGADAVRFIYIMRRADAQMEFDLELAKQKTMDNPVYYVQYGHARVASI
                     VRKATGMGYQVPSFSPDLLSSLTLPEELALVKDLCRFPGIVAQSAANCEPHHVCFFLM
                     ELCKGFHSYYTKYKSSERVVSDDRDKTLARLYLVSRIKSVLGYGLGLLGVTAPEEMRC
                     EGTDVES"
     gene            5260..6465
                     /locus_tag="PKH54_05525"
     CDS             5260..6465
                     /locus_tag="PKH54_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOC99384.1"
                     /translation="MSNPDLENDAPEHAGGSDTARRVNEIELRDFDRIRERTPFQLKP
                     VHVKVGAAAALATIVLAVVAGWMLGRSADKGRPAIQEITGDTKLTSVETVAEGDRPAL
                     DVPTESTGKKRRRVQGSVLPLPGAEPRPSEMVETIRRGLDEVSREAPEVQAFEWWEYF
                     VAINCAFSCLEPYCACSDDLPAACPEPEPAPAVPDEPEPVPEPAREPVPEQVADPGPQ
                     PVPETKPDAAPEPDAVSPAHANAEAEPADGQRPPADAGSKPAAATEGATGPQAPAQME
                     PPVASGEPAGSSSPTPDGAVPEHRQADRKQAVEAARRQAPEKKPVPPTKYSVQIRSFK
                     EEAIAKDFAREFGAKGYDTFVVSYVDPAGTTWFRVRTGHFSTAAEATAFAARVNSKEA
                     EQAIPVEAK"
BASE COUNT         1422 a         2032 c         2025 g         1106 t
ORIGIN      
        1 gccaaccatc acggggcgta ctccctttcc tatgaggcgg tgactcagtc aaccactact
       61 gcccagcctg cccggactcc tgcgggaacc agcgcagcat cgcggcaagg gcggcgccgg
      121 tcagcacgta ggccgccgca agttcaaacg aacgcgcggc ctcgggaaga cgtggcaggt
      181 aaaggccgcc gcccggcgcg accgaatgaa tgacgccgaa caggctgaac acgccaaggg
      241 tgacaaaagt cagcaaaacg gcgagtggct tcctgtctat aagaaagacc agcgcagatc
      301 cccacagaat cgcggtaact atgaacccgt ttccaagaat ggccaggatc ccgagttgtg
      361 ccgccagcga cggatccggc cttgcgccgg cgccaagaag ctggcctgta tagatggtta
      421 tcacataggc caggcaggga atgatcgaga aggccaccgc catcatgtga cgccggggcg
      481 atgcggaaaa tgcctgttcg gtgattgcca ggccaacaaa cagaagaacc ggcgcgacca
      541 cgacctcggg aacgaaatcg atgatgaccc ccagaacgcc gaaaatgccc gcaaggccga
      601 tgacaatgcc ggtaagaaac gtatagcccg ccctggcgcc catcgccttg taagcgggat
      661 gaccgatgta cggggtcgat tgggcgacac cgccaagagc gccggcaata agggtcgcga
      721 ccgattcgac aagaaggatg tcgcgagtcc ggtagtcgtc gccggccgcc ctggccgaag
      781 cggtcgtgtt gattccgccg acgattgtca gaaggccgaa cgggatgctg accggaagat
      841 acctgattgc gccggccatt ccggacgtca gggcggaaag atcgattccc ggccagttca
      901 ggccgattgt agccgagtgg gttacaggtg ccggtgaaag tcccgcaatg gccatgaccc
      961 agtaaatgat caggcctgcc acaacggcca gcagcgcgcc cggaattctc atcggaagcc
     1021 tgaacccggc cacgattgag tacaggatca cgccggtcgc gaaaaggccg gcaaccggcg
     1081 ccgaaaagat gcgcatgaca gggataagac caagcaacgc aaggccaatc ccggcaagcg
     1141 gccccagcag ggccggaagc ggcacgattc gctgcaccca gtcgccggcg aacgacatga
     1201 cgaccttgaa aagacccatg atgaacaagg tcgccatccc gaccgaccag gcggtcattg
     1261 cgttgccgga ctcaagaaat accggcccga tgacggcgat cgtcatgccg atggtcgatg
     1321 gcgtgtcaag gccaaggggc atggccgtca tccttctgcc gcgccggcag gccatccacg
     1381 tgtaggccag atcaccgaac atcacgccga tcgcggttcc aggaatcatt cgcgtcaata
     1441 tgaagtcctg gggcatgccg aactgacccg ccaggatcgc ggtcaacatc acaaggttga
     1501 cgacgttatc gacgaacagg gcgaaaaagg cgttcaggtc gcccgagacg aaccacttgt
     1561 ctgctttcac gggaacttca cccagccggg gaccgacgga ttatacgaca ggccggattc
     1621 aggtacagac tggacggcga aaggattgtc cgtggccgaa ttcggagccg gtatcagaag
     1681 ggaacatcca cgtcgttgat ggcgctgggg gtcgtcaccg gcatgccgac cggctcggcc
     1741 accgggggct cgccgtagga ctggtgcgcg ttgtcagcgc cgccgcggcc gccgatcgac
     1801 tggacgttgc tggcgacgat ttcggtcgtg tagcgcttct ggccgtcctt gtcgtcccaa
     1861 ctcctggtct ggatccgacc ctcgacgaac accgtgctgc ccttctgaag gtactggccg
     1921 cagaattcgg cctggcgccc ccagacgatg accttgtgcc actcggtgcg ctcctgcttc
     1981 tggccggcct tgtcattcca gacctcgttc gtcgcgacac tcagggtcgc gaccgtctgt
     2041 ccaccgccag tcgtgcggat ttccggcgcc gaaccaaggt ttccgatgat gagaacacga
     2101 ttcagggatg ccatgattaa cctcttgcaa aagggtttct tcaaacaccg ccgcggcggc
     2161 gcacgtttcc ttctttcgaa cgggaacccg aagcgttacg caaaaaacac ctcatgcagt
     2221 tcccttgtga ccgaatcgac cttgtccgca tcgacaagaa gcgtcatcgc cgcaggcgcc
     2281 agcatcatcc cgcgaagatg cccgattgac ctgacatgcg aaatcgcggc cgggccggat
     2341 ggatccgcaa gcacgtcacc gaccaccgtg accgcgccga tgtccttgac aagggtggcc
     2401 ccttccggaa gcgacgccgt caggaagccg tccagagcaa ggttgtcggt gcgcgaaaaa
     2461 accatccacg tcccgccgcc gccggccgcg cagacgaagc acggcgtcag tccaccggcc
     2521 gcaagcatcg aaagggcctg gtcggaaacg ccgccatcgg acgaaaagcg aagcgcgacg
     2581 acgtccttgc gggccgtgac gccaacgacc gaacgcggcg gtgcgtcagg attgacgcgt
     2641 actaccgtcc cctctttcaa cgggtccatc gatgaccgtg tgtaaatcgc gattccagcg
     2701 cgccgggcga actcgaccgc atcggcgttc aagaccttcg cgccgtgacg tgacagggaa
     2761 agcatctgtt cgtaatccaa ttcctggaat tgacgggcgt tgatgacgac gcgaggatcc
     2821 gcggaacaca caaacagcac atcggaatat atttcgcacc gttcggcgtt caacgccgct
     2881 gccagcgcga ccgcggtggt atcgctgccg ccacggccaa gcgtcgtgat ttcccttttg
     2941 tagctggttc cctgaaagcc ggccacgatc acgatctttc cagcctcgag ttcgtcgaga
     3001 accctgaacg gccgaacctc gacgattctt gcgtggctgt ggctgtggtt ggtcatgatg
     3061 ccgcactgcg aaccggtcag ggagatcgcg tcctggccca tcgcctggat tgcaatcgaa
     3121 agcagcgcca tcgtgatgcg ttcgccgacg gtaagaagca tgtcgagttc gcgcatcgaa
     3181 ggttccggat tgatgtccgt cgccatccgc atcagttcgt cggtcttgcc ggacatggcc
     3241 gacacgacga ccaccacgtc gtgccctgcc cgcctggtct cgacgacatg acacgcaacc
     3301 ttcttgatcc gctccaggtc ggccaccgaa gttccgccgt atttctggac aatgatcgac
     3361 aaccggtcac ctcgggaatg gacgcaacac gcgaattata ccagcaagcc ggcatttgga
     3421 atcccgaacg cgcgtccgcg ggataataac tgtgcaagaa accggggcgc gcgcgggaaa
     3481 acaaacttgc cgaaacgccc gcgcgccctt ataaggaaag cgacaaatcg atgggggacc
     3541 gttcccgcgc catgaatcgg aattttgaag aaatcgcggc cggcacgctg gccgaggcac
     3601 ttgacaaact gaaggccgca ggcggcgtgc ccgatgactt cgagccaaaa atcgttatcg
     3661 aaaggccgaa gaatccggac ttcggcgact tcggctgcgc cctgccgatg ctgatgtgca
     3721 aggccacaag aaccgcaccc gccatatgcg gcaggaacct gatggacaag atccaggccc
     3781 cgcccggcat cttcgacgaa atctcgttcg cgccccccgg atatatcaac ttccgggtca
     3841 gcccgaccgc atggttcgaa gggcttcagg cggtctgcga catcccggac gtcggccatg
     3901 gacagaaagt ccttcttgaa ttcgtttcgg ccaacccgac aggccccctt cacgccggac
     3961 acgcccgcgg ggccgtactg ggagacatcg tcgcgaggct gatgaaggtc gcgggatacg
     4021 aagttgaaac ggaatacctg cttaacgacg tcggcaacca gatggccatg ctcgggctgt
     4081 cgatttttta tcgtggccga caggcgcttg gactggcatc cgaggtggac ttcccggaaa
     4141 acggataccg tggcgattac atcagggaaa tcgcggcacg tttcgtgtcg ggacaggaag
     4201 gccgtgattt ctgcgcggct tcatacccgg agggtgcggg ccgtgacgac aatcccgcgg
     4261 taaggttcgc ggccggtctg ctgaccgagg aaatccggaa aaccctggac tgccttgcaa
     4321 tcaggttcga ccactggttt tcggaaagaa gccttcacga ttcgggccgg gtggccttcc
     4381 ttgtgaaccg ccttcttgaa tcgggggcgg cctaccggac ggacgacggg gcgatcctgt
     4441 tttcgatgga aggcgacgag gacggggaag atcgcgtcgt cgtgcggggc aacggggttc
     4501 cgacctattt cgcggcagat atcgcctatc acgatgaaaa ggccagacgg ggatatgacc
     4561 gcctgatcaa catatggggc gcggatcacc acggatatat cccaagggtc agggccgcga
     4621 tgggcgcgct tggacacagt ccgtcgatgc ttgacgtgat ccttgtccag atggtgacgc
     4681 ttacaagaaa cggcgaagtc gttcccatgg gcaaacgcct cggacgcttc gtgacgcttc
     4741 aggatctggt cgatgaggtc ggcgccgacg ccgtccgctt catctacatc atgcgtcggg
     4801 ccgacgccca gatggagttc gacctggaac tggcgaagca gaagacgatg gacaacccgg
     4861 tctattacgt ccagtacggc cacgcgcgtg tcgcctcgat cgtgcgaaag gcgaccggga
     4921 tgggatatca ggtgccgtcc ttcagcccgg atctcctgtc aagcctgacg ctgccggaag
     4981 aactggccct ggtcaaggat ctgtgccggt tccccggcat cgttgcccag tcggccgcga
     5041 actgcgagcc gcaccatgtc tgcttcttcc tgatggaact gtgcaaggga ttccattcgt
     5101 actacacgaa atacaagtct tccgaacgcg tggtctcgga cgaccgggac aagacgctcg
     5161 cgcgccttta tcttgtctcg cggatcaaat ccgtgcttgg atacggactc gggcttcttg
     5221 gagtgaccgc ccccgaagag atgcggtgcg agggaacgga tgtcgaatcc tgacctggaa
     5281 aatgacgctc cggaacacgc aggtggctcc gacacggccc gcagggtcaa cgaaatcgaa
     5341 ctgcgcgatt tcgaccgcat tcgtgaacga actccgttcc agttgaagcc ggttcatgtc
     5401 aaagtggggg ccgccgcggc acttgcgacc atcgtgctgg ccgtcgtcgc cggttggatg
     5461 cttggaagaa gcgccgacaa ggggcgcccc gcgattcagg aaataaccgg ggatacgaag
     5521 ctcacgtcgg ttgaaacggt tgccgaaggc gatcgtccgg cgctcgacgt cccgacagaa
     5581 tcgaccggca agaagcgacg cagggttcag ggcagcgtgc ttcccctgcc cggggccgaa
     5641 cctcggccgt ccgagatggt tgaaacgatc cgccgcggcc tggatgaagt gtcccgggag
     5701 gcccccgagg tacaagcctt cgagtggtgg gaatacttcg ttgccatcaa ctgcgcgttt
     5761 tcatgcctcg aaccttattg cgcgtgctcg gacgaccttc cggcggcatg tccggagccg
     5821 gaaccggccc cggcagtacc agacgaaccg gaaccggtgc cggaaccagc ccgggagcct
     5881 gtgcctgaac aggtggcgga ccctggcccg caaccagtgc cggaaaccaa acccgatgcc
     5941 gccccggaac ctgatgcggt ttctcccgcc cacgccaatg ccgaagcgga acctgccgac
     6001 ggacaacggc cgcccgcgga cgcaggaagc aagcctgccg ccgcgacgga aggggccacc
     6061 gggccccagg ctccggcaca aatggaacct cccgtggctt ccggggaacc ggctggctcg
     6121 agttccccga caccagacgg ggctgtcccg gaacacaggc aggccgatcg gaaacaggcg
     6181 gttgaggccg cccgcaggca ggcgcccgaa aagaagccgg ttccgcccac gaagtactcg
     6241 gtgcagataa gatccttcaa ggaagaggcc atcgcaaagg acttcgcccg cgaattcggc
     6301 gcgaaggggt atgatacttt tgtcgtaagc tatgttgacc cggccgggac gacctggttc
     6361 agggtgcgga ccggacattt ctcgaccgcc gcggaagcaa ccgccttcgc ggcccgggtc
     6421 aattcgaagg aggccgagca ggccatcccg gtggaggcaa aatgattcca atcatcgtcc
     6481 cgagaaaggt cgaaaagccg tggggctggg aactctgggt cgccgtgacc gagaaatacg
     6541 cgggaaaaat catccacatc aacccgggcc agaagctttc aaggc
//
LOCUS       DAOPPA010000134         6555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12895_length_6555_cov_4.025538, whole genome shotgun sequence.
ACCESSION   DAOPPA010000134 DAOPPA010000000
VERSION     DAOPPA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12895_length_6555_cov_4.025538"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKH54_05530"
     CDS             complement(<1..245)
                     /locus_tag="PKH54_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOC99385.1"
                     /translation="MSDKPVRVRIAPSPTGDPHVGTAYVGLINYAFARQNGGQFILRI
                     EDTDQERSTRASEQAIFDALRWVGLSWDEGPDVGGPLG"
     gene            complement(344..952)
                     /gene="gmk"
                     /locus_tag="PKH54_05535"
     CDS             complement(344..952)
                     /gene="gmk"
                     /locus_tag="PKH54_05535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOC99386.1"
                     /translation="MKGILVIISAPSGAGKSTIIRQILGNDPNCRFAISHTTRQPRAG
                     EKDGVDYYFVGRDEFQSMVANDRFAEWAVVHSNFYGTSLAEVERLSSQGIDVVFEVDY
                     QGGRALMRRFPDAASIFVLPPSMAEVKRRLTDRGTDDAETIEMRLQNARIEIATAGEY
                     RYIVVNDDLDRAVSQVGDVIRVERLRSINRAAMIRDLVAEKV"
     gene            complement(1058..1720)
                     /locus_tag="PKH54_05540"
     CDS             complement(1058..1720)
                     /locus_tag="PKH54_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOC99387.1"
                     /translation="MNDVNESSSSWSDSRLIASVISDGRCGREAIRRADELLRGYGSA
                     RLVVAAVDASGRPVGHRLTPAEQRRLADARELARRLLAAEISDGTNRVSTPAEVASWA
                     GRLAFCREEVLMVVGLDTRNRVAGSWEIARGWEAGINIHPRQIFSLLIRESVGRCVLV
                     HNHPSGNPEPSDEDIRFTRTIIDAGRLLGVDVLDHVVVAASGFFSMRADCVNLEFGRC
                     SS"
     gene            complement(2036..2114)
                     /locus_tag="PKH54_05545"
     tRNA            complement(2036..2114)
                     /locus_tag="PKH54_05545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2076..2078),aa:Pro,seq:ggg)
     gene            2319..3869
                     /locus_tag="PKH54_05550"
     CDS             2319..3869
                     /locus_tag="PKH54_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99388.1"
                     /translation="MKNIRFALCASFLVVAFILPAMASSGESPRPEAARTAGQSVASC
                     IPSCDGRTCGPDGCGGACGQCRRGAACVTGRCVDGAVDVTVDYTGLVGDIFPTFIAHL
                     LGSGPLNDVPLVSVKARNLSDSDRVPLSCHVDIPGFSAAGKSTINLLPGESRVIRLTP
                     TLDFAALSRITTSTPSAVNVSIKTANSMIFEETRPIKLASKNSMFWSFPNEAPDESQP
                     KMKRNHPLAPFLAAFVVPHDKDFLIRKLLKSASDFMPDKAMVGYQDVPLEPTEIEALR
                     TSDFAVETIRPGANNVVQIRLMQIPGAGYPDVAFQVMDDANLAAWKAGQEAAVYFAQA
                     DAVAAGQMEFKSEADIDYHLVYSRGPRFTATSRVARVRTATHTEVVTMQASAIFKAMK
                     ALGVNYVNAPNDFFSTSQFIMYPSETLAQKTANCAEGALVFAAAFKALGLRAVLYLVP
                     GHMFVGVRTWHNEDLIVPIETTMVASGEPDQAIEVAMDTVKKTPPDDIMIIDVDQMAA
                     IGVVPWPL"
     gene            3901..5034
                     /locus_tag="PKH54_05555"
     CDS             3901..5034
                     /locus_tag="PKH54_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99389.1"
                     /translation="MKFTDGFVPFLAMVALLTACGEEAGGPKDPFGPMSPTTVSTLAA
                     TDSQGRSMQLTVRQNETVVVNGREYTVLAVGRDPTGVSTDFKAFIDLKPDSVTFAGGE
                     INWGENGGFPFVSGTTDTPVFVNLDPPLDVDQTVPIKGTAYLGDPSDPANGTQLETTM
                     TYRAIEKDTRVVTQAGVFNGVTRFTGLTNVDGTVINGEAWYHPDFGMLKGTIDWPQPY
                     GSTLDLTGMTDVGSNGPGANTVRKMGVIDYSNTHWDISTGEVGDGWTADRTKHAKMLL
                     EMRFVDDSAAMDPNLVPQVDVEFGTVWGYFYHQLVASPISFFHPEDNGKGYTFWVAYV
                     DQAGNKHDPGTEIYHIDVDMMPGNPGPIQITGRIIYQKAPGVE"
     gene            complement(5066..5671)
                     /locus_tag="PKH54_05560"
     CDS             complement(5066..5671)
                     /locus_tag="PKH54_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOC99390.1"
                     /translation="MMKKVIAIAAGFVAVMFLAFAPVAASAQGCGGCKGKGAEMKDGM
                     GPHGHHHGHKKDFRFWKCPKASAELKLTPEQIKKLDAADEAAGKKMDAIRDQLKTLKA
                     QKHDAMKGHPVDRDKVMGLVAKVIDLKSQKMKARAEIKLEAFGILTKDQKLKLDSLRD
                     EMMPKGPGCDCGAGCGCKDGDKGDGKACNCGCGGGCGCDKD"
     gene            5872..>6555
                     /locus_tag="PKH54_05565"
     CDS             5872..>6555
                     /locus_tag="PKH54_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC99391.1"
                     /translation="MRNEGFSEKPARILIIDDDQKLCSLVRDYLAPHGYDVAAAFSGP
                     AGLDAAMKGDFKAIILDVMLPGMDGFEVLQQLRREVNTPVLMLTSRGDETDRIVGLEM
                     GADDYVPKTFSTRELLARLRAVIRRAGTGSVNRALSDQIVEVGPVRVDHLARVARLNN
                     EPIDLTPVEFELLFQLARSPGRVLSRDQLLDRVSGRDYDVYDRSIDVHVSSLRRKLND
                     DPRCPKFIKT"
BASE COUNT         1307 a         2041 c         1850 g         1357 t
ORIGIN      
        1 cccaaggggc cgccaacgtc ggggccttcg tcccacgaca ggccgaccca gcgcagcgcg
       61 tcgaaaatcg cctgttcgct ggcccgtgtc gatctctcct gatcggtgtc ttcgatgcgc
      121 aggatgaact gtccgccgtt ctggcgggca aatgcgtaat tgatcaggcc gacgtaagcg
      181 gttcccacat gggggtcgcc ggtcgggctg ggtgcgattc gtacacggac tggtttgtcg
      241 gacattgaac ctcctgaaac acaaaaacgg ccgaatatga ttcgcccgct ccccgatctg
      301 taagggcgaa tgaaaattcg cccgctccaa tcaaattcgc ccgctaaacc ttctcggcga
      361 caaggtcgcg aatcatcgcc gcgcggttta tcgaccgcag tctttcgacc cttatcacgt
      421 cgccgacctg ggaaaccgcc cgatccagat catcgttgac gacgatgtac cggtattccc
      481 cggcggtcgc aatctcaatc cttgcgttct gcaggcgcat ctcgatggtt tcggcatcgt
      541 ccgtgccgcg atcggtcagg cgccgcttca cctcggccat cgacggtggc aatacgaaga
      601 tgcttgccgc atccgggaaa cggcgcataa gggcccgccc gccctggtaa tcgacctcga
      661 acacgacatc gatgccctgg cttgaaagcc gctcgacctc ggccagcgaa gtaccataaa
      721 aattcgaatg gaccacggcc cattccgcga acctgtcgtt cgccaccatg gactgaaact
      781 catccctgcc gacgaagtag taatcgacgc cgtccttctc ccctgccctt ggctggcggg
      841 tcgtatgcga aatggcgaag cgacagtttg gatcgttccc aagaatctgc cggatgatgg
      901 ttgacttgcc ggcgcccgac ggcgcgctga tgattaccag gatacctttc attggcggcc
      961 cccgctggct gaatgtccgg cctgcccgac cctgcgggat tcccggctgg acgtcgcttg
     1021 ccgaaacgcg tgcaacccgt tccggcgcgc gacgatgcta gcttgaacac cgcccgaatt
     1081 caagattgac gcaatcggcg cgcatgctga aaaatccgga cgcggcaacc acaacatgat
     1141 ccagcacgtc gacgccaagc agccttccgg catcgataat ggtccgggtg aaccggatgt
     1201 cctcgtcgct gggctcgggg tttccggacg ggtggttgtg caccagcacg caccgcccga
     1261 ccgattcacg gataagcagc gagaatatct ggcgtggatg tatgttgata ccggcctccc
     1321 agccgcgcgc aatttcccac gaaccggcga ccctgttcct ggtgtcgagc ccgacaacca
     1381 tcaacacttc ctcccgacag aaagcgagcc gccctgccca cgaggcgacc tccgccggag
     1441 tggaaacccg gttcgttccg tctgaaatct cggccgccag aaggcgcctt gcaagctcgc
     1501 gagcgtccgc aaggcgccgc tgctcggcag gtgtcagcct gtgcccgacc ggacggcctg
     1561 aggcgtcaac ggcggccacg acaagccttg ccgagccgta cccccgcagc agttcatcgg
     1621 ccctcctgat tgcttccctg ccacaccgtc cgtccgaaat cacgcttgcg ataagcctgc
     1681 tgtcggacca acttgaactt gattcattca cgtcattcat tgccgcaccg tccgattgag
     1741 tcggatgctg acgttacagg aaccgtgccg ttcactttcg caggccgtca gtctcgaaac
     1801 aaaccgcgta aattcaaatt cttatccgaa tccaggtgat ggctgccagg cgagactgac
     1861 ttcaataaat gttggacatg gagcgtggtt acgcgcggac tttgacccca ggttccgtcg
     1921 gatcgcgaga ccgaccgggg aaacgaggcg ccgcagaacc ggccgaagtg cccgacagta
     1981 cccggccgtc agtccacaac acgaccaggc aacgcgaaaa cgacaacaat tgaattggtc
     2041 ggggcgactg gattcgaacc agcgactcct tgacccccag tcaagtgcgc taaaccaggc
     2101 tgcgctacgc cccgtaaaaa gcaggtggat gataccagcg ttgattcgac gcggcaagtc
     2161 ggaagttcgt tggggtcaca ccctcacttt tcattggctg gaagccctcg tcgtcccggc
     2221 tgcccgtctg ttgtcaacca ggctgtgggc tggtacaatc cggcctgcca tcagcgcttt
     2281 tcaagtcgat tgtatttatg ggcatgaggt tcttccgtat gaaaaacatc cgtttcgcac
     2341 tctgtgcgtc gttcctggtc gtggcgttta ttcttccggc gatggcatcg tccggcgagt
     2401 cgccccgccc ggaggcagcc agaaccgcag gccagtccgt cgccagttgc attccgtcat
     2461 gcgacgggcg gacgtgcgga ccggacgggt gcggaggggc ctgcggtcag tgcaggcgtg
     2521 gagcggcatg cgttaccggt cgctgcgtcg atggcgccgt cgatgtgact gtcgattaca
     2581 ccggtcttgt tggtgacatc tttccgactt tcatcgcaca cctgctgggc tccggcccgc
     2641 tgaacgacgt tcctcttgtt tccgtgaagg cccgcaacct ttccgacagc gaccgggttc
     2701 ccctttcgtg ccacgttgat atccccggat tcagcgccgc cggcaaatcg accatcaatc
     2761 ttcttccggg cgagagccgg gtgatccgtc tgacgcccac gctcgacttc gcggcgctgt
     2821 cgcgaataac caccagcacc ccgtccgcgg tcaacgtcag catcaagacc gcgaactcga
     2881 tgattttcga ggaaaccagg ccgatcaagc ttgccagcaa gaacagcatg ttctggtcgt
     2941 tcccgaacga ggcgccggac gagtcccagc caaagatgaa gcggaatcat ccccttgcgc
     3001 ccttccttgc cgccttcgtc gtgccgcacg acaaggattt cctgattcgg aagctgttga
     3061 agtccgcatc cgatttcatg ccggacaagg caatggttgg ttaccaggac gttccacttg
     3121 aaccgaccga aatcgaggcg ctgcgtacaa gcgacttcgc cgttgaaaca ataaggcctg
     3181 gcgccaacaa cgttgtacag attcgattga tgcagattcc cggggcggga taccccgacg
     3241 tcgcgttcca ggtgatggac gacgccaatc tggcggcatg gaaggccggc caggaagcgg
     3301 cggtctattt cgcccaggcc gacgcggttg ctgccggcca gatggaattc aagtccgagg
     3361 ccgatatcga ttatcacctt gtctattcca gggggccgcg tttcacggcc acttcacgcg
     3421 tggcccgggt caggactgca acccacaccg aagtggtaac catgcaggca tccgcgattt
     3481 tcaaagcgat gaaggccctg ggcgtcaact atgtcaacgc ccctaacgac ttcttttcca
     3541 cttcccagtt catcatgtat ccgtcggaaa cgcttgcgca gaagacggcg aactgcgccg
     3601 aaggggcgct ggtgttcgcg gccgcgttca aggcacttgg gctgagggcc gttctttacc
     3661 tggttcccgg gcacatgttt gtcggggtca ggacctggca caacgaggat ctgattgttc
     3721 cgatcgaaac gaccatggtt gcgtcgggcg aaccggatca ggccatcgag gttgccatgg
     3781 acaccgtcaa gaagactccc cccgatgaca tcatgataat cgacgtcgat cagatggcgg
     3841 caattggcgt ggtgccgtgg ccgctttgac cctgtcgtac tttcaaatgg aggcggcccg
     3901 atgaagttca cagatggatt tgtcccgttt cttgcgatgg ttgcgctgtt gaccgcgtgt
     3961 ggcgaggaag ccggcggacc aaaggatccc ttcgggccaa tgtcgccgac caccgtttcg
     4021 accctggcgg cgaccgacag tcagggccgc tccatgcagc ttacggttcg tcagaacgag
     4081 accgttgtcg tgaacggtcg tgagtacacg gttctggcgg ttggacgcga tcccaccggc
     4141 gtttccacgg acttcaaggc tttcatcgac ctgaagccag attccgtcac cttcgccggc
     4201 ggggaaataa actggggcga gaacggcgga ttcccgttcg tatcaggaac cacggacaca
     4261 ccggtttttg tgaaccttga cccgcccctt gacgttgacc agacggttcc gatcaaggga
     4321 accgcgtatc ttggcgaccc atcggatccg gcgaacggca cgcagcttga aactacaatg
     4381 acataccgcg ccatcgagaa ggatacgcgt gttgtcaccc aggcgggcgt tttcaacggg
     4441 gtcacgcggt tcacgggatt gactaacgtc gatggcacgg tcatcaacgg cgaggcgtgg
     4501 taccaccctg acttcggcat gctgaaggga acgatcgact ggccgcagcc ttacggctcg
     4561 acgctggatc ttaccggcat gacggatgtc ggctcgaatg gacctggagc caacacggtt
     4621 cggaagatgg gtgtgattga ctactcgaat acccactggg acatcagcac gggcgaagtc
     4681 ggggacggat ggaccgccga tcggaccaag cacgcgaaga tgctgctgga aatgcgtttc
     4741 gttgacgatt ccgcggccat ggatccgaac ctggtgccgc aggtcgatgt cgaattcggg
     4801 acggtctggg gatacttcta ccaccagctt gtagcttcac cgatttcgtt cttccatccc
     4861 gaggataatg gcaagggata tacgttctgg gtggcctacg tcgatcaggc cggcaacaag
     4921 catgatcccg gcaccgaaat ctaccacatc gatgttgaca tgatgcccgg gaacccaggc
     4981 ccgatccaga tcacgggaag aatcatctat cagaaggcgc ccggggtcga atgaggggaa
     5041 tcggttccgc cgaaacgtac cgacatcaat ccttgtcgca cccgcatccg ccgccacagc
     5101 cgcagttgca agccttgccg tcgcccttgt cgccgtcctt gcagccgcaa ccagcgccgc
     5161 aatcacagcc ggggcccttt ggcatcatct cgtcgcgaag cgaatccagc ttcagcttct
     5221 gatccttggt caggatgccg aacgcctcga gcttgatctc ggccctggcc ttcatcttct
     5281 gcgacttaag gtcgatgacc tttgcaacca ggcccatcac cttatcccgg tcaaccgggt
     5341 gtcccttcat cgcgtcgtgc ttctgggcct tgagcgtctt caactgatcg cggatggcat
     5401 ccatcttctt tccggcagcc tcgtccgccg cgtccagctt cttaatctgc tctggcgtca
     5461 gcttcagttc agcactcgcc ttcgggcact tccaaaagcg aaaatccttc ttgtggccat
     5521 gatgatggcc atggggcccc atgccgtcct tcatctcggc gcccttaccc ttgcaaccgc
     5581 cacacccctg ggccgaagcc gccacgggcg cgaacgccag gaacatcacc gcgacaaaac
     5641 ctgccgcaat tgcaataacc ttcttcatca tcactcctcc agacaacagc agagcaccgc
     5701 ctgcgaccgt cgcgacggta ccgttaataa tataaagcgg attcaatttg tccagattga
     5761 tcttcaaatc cttttgtaaa tttagtgtaa ttggaattgc ccatttaacg ccgcactgta
     5821 ttgtaaaatc taacgcagtt gcgtccgctc attgcggcag aggggttgtc catgcgaaat
     5881 gaaggttttt cggagaaacc ggcgcgcatc ctgattatcg acgacgacca gaagctttgt
     5941 tcgcttgtcc gggactatct tgccccgcat gggtatgacg tggcggcggc cttcagcggg
     6001 cccgccggcc tcgatgccgc gatgaagggt gacttcaagg caatcattct tgacgtgatg
     6061 ctgcccggaa tggacgggtt cgaggttctt cagcagcttc gccgcgaggt caataccccg
     6121 gttttgatgc tcacgtcccg cggcgatgag accgatcgca tcgtcggcct cgagatgggc
     6181 gccgacgact acgtgcccaa aaccttttca acccgggaac tccttgcccg gctgcgcgct
     6241 gtgattcgcc gcgcgggcac cggttcggtc aatcgagccc tgtccgatca gatcgtcgag
     6301 gttggcccgg ttcgcgtcga tcaccttgcc agggtggccc gactgaacaa tgaaccgatt
     6361 gatctgaccc cggtggaatt cgagcttctt ttccagctcg cgcgctcgcc cgggcgcgtt
     6421 ctttcccggg atcagcttct ggatcgcgtt tcgggccgcg actacgacgt ttatgacaga
     6481 tccatcgacg tgcatgtttc ttcacttcga cgaaaattga acgacgaccc acggtgtccg
     6541 aagttcatta aaact
//
LOCUS       DAOPPA010000135         6527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12959_length_6527_cov_3.815513, whole genome shotgun sequence.
ACCESSION   DAOPPA010000135 DAOPPA010000000
VERSION     DAOPPA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_6527_cov_3.815513"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKH54_05570"
     CDS             <1..250
                     /locus_tag="PKH54_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99392.1"
                     /translation="NGRMRVAVRNDGPGFSPADRRSLFKKFSRLKSPELMKRKGTGVG
                     LYTVWRVISLHGGRVDAVSEEGSWAEFSFEIPVAGKAT"
     gene            289..558
                     /locus_tag="PKH54_05575"
     CDS             289..558
                     /locus_tag="PKH54_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99393.1"
                     /translation="MHEHYILGVHVTNRTVNSVEVQKVFSEYGCYIKTRVGLHDVDET
                     GGLCSSTGIVVLEIIGGRAIVDQIIAKLKPIAGIQTELMVFSHPQ"
     gene            568..1977
                     /locus_tag="PKH54_05580"
     CDS             568..1977
                     /locus_tag="PKH54_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOC99394.1"
                     /translation="MADDDDGYRVESDPLGQVRIPLDCLWGVHTVRAIDNFPLSGRPV
                     NPALIRAFGEVKLAAAQVNRELGSWNSDTRKAEAIEIACREMADGQLDEHVVVDAMQG
                     GAGTSTNMNVNEVIANRSMRILGLRPGEGSRVSPLDDVNLHQSTNDTYPTALRIAAIR
                     GVRRLDGALVSLQEAFQDAERRFGDVVKLGRTQLQDAVLTTLGREMGAYAEAFGRDRW
                     RVYKCEERLRVVNLGGTAIGTGLGAPRKYIFRVVDVLRQNTGIGLARAENLVESTQNA
                     DVFVEVSGILKACAVSLVKVSSDLRLMASGPDGGLREIRLPARQAGSSIMPGKVNPVI
                     PEAVTQAAMKVMGNDTVIAHACSMGVLELNPFLPIVADALLESIDLLVNACEIFRRNC
                     ISGIEADVDACRAHVDSSTAVATALIPVLGYGRVSELVSRSRATGRQIKEIAIEEGFV
                     TAQEYDMLLTPEAVKRLGW"
     gene            1993..3267
                     /gene="hydF"
                     /locus_tag="PKH54_05585"
     CDS             1993..3267
                     /gene="hydF"
                     /locus_tag="PKH54_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOC99395.1"
                     /translation="MSMNTPRGFRLHIGLFGRRNVGKSSVLNMITRQQVSIVSEFAGT
                     TTDPVEKPMELLPLGPVLFIDTAGLDDVGALGELRVKKTIQVFDRTDLGVIVVAAGDW
                     GDFEQHILDELKGRNIPVVVIFNKSDIALPSADLQDRLAAAKARIVKVIAASSDPAVN
                     GLADVRQALLDAAPSEFVDNPSILGDLVPSGQMAVLVVPIDKEAPKGRLILPQVQSIR
                     DLLDNDAYCVVVKERELRAALEMLKRPPALVVTDSQAFLRVAADTPPEIPMTGFSILF
                     ARFKGDLAEMVRGAVAIERLKSGSRILIAESCSHHPISDDIGRVKIPRWLTQYVGGKL
                     DIVHVQGHDFPTTDEGLAGYELVIHCGGCMWNRREMLSRILRCRRAGVPITNYGLTIA
                     YSLGIFERALSPFPGALEAYRLALAGDTPKES"
     gene            3267..4328
                     /gene="hydE"
                     /locus_tag="PKH54_05590"
     CDS             3267..4328
                     /gene="hydE"
                     /locus_tag="PKH54_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOC99396.1"
                     /translation="MLDRDAVIAWLRESDQSRLFELFAWADRVRHETVGDAIHLRGLL
                     EVSSFCVRQCAYCGINATNRTARRFRMDRDEILAAAREAKGYGYGTIVMQGGEDYGIK
                     AEWMADIIRAMKQDVGLAITLSLGERRPDELKLWKEAGADRYLLRFETSDPALYRLIH
                     PALPGGFEDRIQQIRMLQEIGYEAGSGVMVGIPGQSWDSLADDIMLFSTLDLDMIGVG
                     PYIPHPDTPMGAWMLEGRGEVAFPKLPEGEQVPGNAETAFKVIALTRLLCPDSNIPST
                     TAIATLNREGGRRQGLMCGANVVMPNLTPAKYRECYEIYPNKATLVETAGVCARNLKD
                     SFEQMGRFIGSGAGGRVRH"
     gene            4396..5829
                     /locus_tag="PKH54_05595"
     CDS             4396..5829
                     /locus_tag="PKH54_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC99397.1"
                     /translation="MRGPLHRLPFSWALGLSLFSGRRKSRVSAATVVSIAGIAVGVAT
                     LTAVMSVTGGFEDVFRDRILGVYPHMVVMNRGDSFPEYQSVSETLKRVPGVIGVAAST
                     YDEMMVSSDTGTSQAIVKGVDLEGIDRVSGLSSLLRGHDLSILKWDGNGPVPVAIGCE
                     LMSSLEVAPGGIIGLTTPIRGMGQGISGGLVMTPREAKFVVVDCVDSGFYDYDSKLVV
                     MELGAAQHFLDRGQIVRWVEVKLNDLFQTESMKSEILRALQPYTIAGVARDVIELQKS
                     VAGTFQAFGIGDGTLDPASASVMIQRARRSLLYSDIGFGPVQNYRVLDWKDMNRNLFS
                     ALRIQKVVIGLFFLIIVVVAAFNIVGTQLIVARERMKDISTLVALGASRMQLWKVFVA
                     HGFVMGCIGVLFGVGLGRAVVAVINSIDFRLDARVYMISQLPATLNWADIALVAFGSA
                     AVVLLSCVFSSLRAIRINPVDGLRRIV"
     gene            5851..6351
                     /locus_tag="PKH54_05600"
     CDS             5851..6351
                     /locus_tag="PKH54_05600"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOC99398.1"
                     /translation="MPAVVSTSLGMVLIDWTSAGICRVVPGVAGPLCPVGPEAPSFVH
                     QAVSDIVGHLDGNVRNLRDIPVDFGDAPPFFKKIWTVLRNNSNPGDIMTYGQLARAAG
                     SPGAARAAGSSMRANPVPIIVPCHRVVGASGPGGYSAPGGLDTKRHLLAIEGVALRLS
                     GRTGVQ"
BASE COUNT         1172 a         1904 c         2076 g         1375 t
ORIGIN      
        1 taacggcagg atgcgcgtcg cggtgcgaaa cgacggcccc ggattttcac cggccgaccg
       61 ccgcagcctt ttcaagaagt tttcacgcct gaagagcccc gagctgatga agcgcaaggg
      121 taccggtgtc ggcctttaca cggtctggcg ggtgatttcc cttcacggcg gtcgtgtcga
      181 tgccgtttcc gaagagggaa gctgggccga gttcagtttc gagatcccgg tcgccggaaa
      241 ggcgacctga cattatttaa gcgggctgat gccccgaagg agtctgacat gcacgaacat
      301 tacatccttg gcgttcatgt gacgaaccgc acggtcaact cggtcgaggt tcagaaggtc
      361 ttttccgaat acggctgcta tatcaagacg cgtgtcgggc tgcatgacgt cgatgaaacc
      421 ggcggattgt gcagttcgac cggcattgtc gtcctggaaa ttatcggcgg gcgcgcgatc
      481 gtcgatcaga tcatcgcgaa gctgaagccg attgccggga tccagaccga acttatggtc
      541 ttttcacatc cgcagtaggc caggctgatg gcagatgacg atgatggata ccgggtcgag
      601 tccgacccgc ttggacaggt gcgcattcca ctggattgtc tgtggggcgt tcataccgtc
      661 cgcgcgatcg acaattttcc cttgtctggc cgtccggtga acccggcgct gatccgggcc
      721 ttcggtgaag tgaagctggc cgcggcccag gtcaaccggg aactcggctc atggaattcc
      781 gacaccagaa aggcggaagc aatcgagatt gcctgtcgtg aaatggccga cggccagctt
      841 gatgagcatg tcgtggtcga tgccatgcag ggcggcgccg gaaccagcac gaacatgaac
      901 gtcaacgagg tcatcgcgaa ccgctcgatg cggatactgg gattgcggcc cggcgaaggg
      961 tcgcgtgtct cgccgcttga cgacgtcaat cttcaccagt caacgaacga tacctacccg
     1021 actgcgctgc ggattgccgc cattcgcggg gttcgcaggc tggatggggc cctggtttct
     1081 ctgcaggagg catttcagga tgccgagcgc aggttcggcg atgtcgtgaa gcttggtcga
     1141 acccagcttc aggatgccgt ccttacgacg ctagggcggg agatgggcgc gtacgccgag
     1201 gcgttcggac gtgatcgctg gcgtgtctat aaatgcgagg aaagactgcg tgtcgtcaat
     1261 cttggcggca ccgccatcgg caccgggctt ggcgccccgc gcaagtacat cttccgcgtg
     1321 gtcgatgtgt tgcggcagaa caccggcatc ggtctggcac gcgccgaaaa cctggtcgaa
     1381 tcgacacaga atgccgacgt gttcgtcgag gtatccggca tcctgaaggc gtgcgcggtt
     1441 tcgctggtca aggtttcgtc cgacttgcga ctgatggcat cggggcccga tggcggcctt
     1501 cgtgaaatca ggcttccggc acgccaggcc ggctcgtcga tcatgccggg caaggtcaac
     1561 ccggtgatac ccgaggcggt cacccaggcc gctatgaagg tcatgggtaa cgacacggtc
     1621 atcgcgcacg cctgttcgat gggagttctt gaattgaatc ctttcctgcc gatcgttgcc
     1681 gatgccctgc ttgaaagcat cgatttgctg gtcaacgcct gcgaaatctt tcgtcgcaac
     1741 tgcatttccg ggatcgaggc cgacgtcgat gcatgccgcg cccacgttga ttcctcaacg
     1801 gccgttgcca ccgccctgat tccagtcctc ggatatgggc gggtttccga actggtttcg
     1861 cgatcccgcg caacaggccg acaaatcaag gaaattgcga ttgaagaagg ttttgtgacc
     1921 gcgcaggaat acgatatgct gctgacgccc gaggcggtca agcgcctggg ctggtgagtt
     1981 gaaggtgcag ccatgtcgat gaacactccg cgcggattcc gtcttcacat cggcttgttc
     2041 ggccgccgaa acgtgggcaa gtccagcgtc ctgaacatga ttaccaggca gcaggtttca
     2101 atcgtttccg agtttgccgg cacgacgacc gatccggtcg agaagccgat ggagcttctg
     2161 ccgcttggtc cggttctgtt catcgacacg gccgggctgg acgatgtcgg ggcgcttggc
     2221 gaactgcgcg tgaaaaagac catccaggta ttcgatcgga ccgacctggg cgtcattgtc
     2281 gtcgcggccg gcgactgggg cgatttcgaa cagcacatcc ttgatgaact gaaggggcgg
     2341 aacatcccgg tcgtcgtgat cttcaacaag tccgacatcg cgcttccatc cgcggatctt
     2401 caggacaggc tggccgccgc aaaggcccga atcgtcaagg taatcgcggc atcttcggat
     2461 ccggccgtca acgggcttgc cgatgttcgg caggcgctgc tggatgcggc gccgtcggag
     2521 ttcgtcgata atccgtccat cctgggcgat cttgtgcctt ccggacagat ggccgtcctg
     2581 gtcgtcccga ttgacaaaga ggcgccgaag ggacgcctga ttctgcccca ggtacagtcg
     2641 attcgcgacc tgctggacaa cgacgcgtac tgcgtggtcg tgaaggaacg cgaactcagg
     2701 gcggcccttg agatgctgaa gcgcccgccg gcgcttgtcg tgaccgattc ccaggcattt
     2761 cttcgagtcg ccgccgacac gccgccggaa attcccatga ccggcttttc gatccttttc
     2821 gccaggttca agggtgatct ggccgagatg gtgcgtggcg ccgtcgccat tgaacgcctg
     2881 aagtccggtt cgcgcatcct gattgccgag tcgtgttccc accacccgat ttccgacgac
     2941 atcggccgtg taaagattcc aagatggctt acgcagtacg tcggcggcaa gctggatatc
     3001 gtacacgtcc agggccacga ttttccgacc acggacgaag ggcttgccgg atatgaactg
     3061 gtcatccact gcggcggctg catgtggaac aggcgcgaga tgctttcaag gatactgcgc
     3121 tgtcgaaggg ccggcgtgcc gataaccaac tacgggctga ccatcgcgta cagcctgggg
     3181 atattcgaaa gggcgctctc gccgtttccg ggggcacttg aagcatacag gctggcgctt
     3241 gcgggcgaca cgccaaagga atcgtgatgc ttgaccgtga tgccgtcatc gcctggcttc
     3301 gcgaaagcga ccagtccagg cttttcgaac ttttcgcgtg ggccgaccgc gtccgccatg
     3361 aaaccgtcgg cgatgccatc cacttgcgcg gcctgctgga ggtgtccagc ttctgtgtcc
     3421 gccagtgcgc gtattgcgga atcaatgcga caaaccgcac cgcccggcgt ttccgcatgg
     3481 atcgcgatga aatcctggcg gccgcgcgcg aggccaaggg ttacgggtac ggcacaatcg
     3541 tgatgcaggg cggcgaggac tacggcatca aggccgaatg gatggccgac atcatccggg
     3601 caatgaagca ggacgtgggc cttgcaataa ccctgtcgct tggcgagcgg cgtcccgacg
     3661 aactgaagtt gtggaaggaa gccggcgccg acaggtacct gctccggttc gaaacttccg
     3721 atccggcgct gtacaggctt atccacccgg cccttcccgg cgggttcgaa gaccgcatcc
     3781 agcagatacg catgttgcag gaaatcggtt acgaggccgg cagtggcgtc atggtcggga
     3841 ttccgggcca gtcgtgggat tcgcttgccg acgacatcat gctgttttcg acccttgacc
     3901 tggacatgat cggcgtcggt ccgtacatcc cgcatcccga cacgccgatg ggtgcctgga
     3961 tgcttgaggg ccggggcgaa gtcgccttcc cgaaactgcc ggaaggcgaa caggttccag
     4021 gcaatgccga aacggcattc aaggtaatcg cgctgactcg gcttctgtgc ccggattcga
     4081 acataccgag cacgaccgcg atcgcgacgc ttaaccgcga gggcggcagg cgccagggtc
     4141 tgatgtgcgg cgccaacgtc gtgatgccga acctgacgcc ggcgaagtac cgcgaatgct
     4201 atgagattta tccgaacaag gcgacgctgg tcgagacggc gggcgtctgt gccagaaatc
     4261 tgaaggattc gttcgaacag atggggcgtt tcatcggctc cggcgccggg ggccgcgtcc
     4321 gccactgaca tttgcctacc ggttcggtcg attgcggcct acaatacccg cgtcttgctt
     4381 ttcgcagggt gatgcttgcg cggtcccctt cacagactgc cgttttcgtg ggcactcggg
     4441 ctgtcgctgt tttccgggcg aaggaagtcc agggtgtctg ccgccacggt cgtgtcaatc
     4501 gccggaatcg cggtcggggt tgccacgctg accgcggtga tgtccgtcac cggcgggttc
     4561 gaggacgtgt tccgggatcg tatcctgggc gtgtatccgc acatggtcgt catgaaccgt
     4621 ggcgacagct ttcccgaata ccagtccgtt tcggaaacgt tgaagcgcgt ccccggcgtc
     4681 attggcgtcg ccgcatcgac atacgacgaa atgatggttt cgtccgatac cggcacgtcc
     4741 caggcgattg tcaaaggcgt tgatcttgaa ggaatcgacc gcgtgtccgg cctttcgtcg
     4801 ctgctgcgcg ggcacgacct ttccattctg aagtgggacg gcaacggtcc ggttccggtc
     4861 gccatcggat gcgaactgat gtcttcgctg gaggtcgccc cgggcggcat catcggactg
     4921 acgaccccga tccgtggcat gggccagggt atttcgggcg gcctcgtgat gactccgcgt
     4981 gaagcaaagt tcgtcgtggt ggactgcgtt gactccggtt tctacgatta cgactccaaa
     5041 ctggtcgtga tggaactggg cgcggcccag cattttctgg atcgtgggca gattgtccgc
     5101 tgggtcgagg tcaagctgaa tgatcttttc cagaccgaaa gcatgaagtc agagatcctg
     5161 agggcgctgc aaccttacac aatcgccggg gtggcccgcg acgtgattga actgcagaag
     5221 tccgttgccg gcacattcca ggctttcggt atcggtgacg gtacgcttga tcccgcttcg
     5281 gcgtcggtca tgatccagcg tgccagaaga tcccttcttt acagcgatat cggattcggg
     5341 cctgtgcaga attacagggt tctggactgg aaggacatga accgcaacct tttttccgcc
     5401 ctgcgcatcc agaaggtcgt gatcgggctg ttcttcctga tcatcgtcgt cgtcgccgcg
     5461 ttcaatatcg tcgggacgca gcttatcgtc gcccgtgaac gcatgaagga catttccacg
     5521 ctggtcgcgc ttggcgcttc caggatgcag ttgtggaagg tgttcgtggc ccacggcttc
     5581 gtgatgggct gcatcggcgt gcttttcggc gtcggactcg gtcgggcggt cgtggcggta
     5641 atcaattcaa tcgatttcag gctggacgcg cgggtctata tgatcagcca gcttccggcc
     5701 accttgaact gggccgacat cgcgctcgtc gcgttcgggt cggccgccgt ggttcttctt
     5761 tcctgcgtct tttcttccct gcgcgccatc aggatcaatc cggtcgatgg gctgcgcagg
     5821 atcgtctgac ggcggatctt gcggggtgtg atgcctgcgg tcgtttcaac cagccttgga
     5881 atggtgctta tcgactggac cagtgcgggc atctgccggg tcgttcccgg tgtcgccggg
     5941 ccgctttgcc cagtcggtcc ggaagccccg tccttcgtgc accaggccgt ttcggacatc
     6001 gtgggtcacc tggatggcaa cgtgcggaat ttgcgtgata ttcctgtgga tttcggggac
     6061 gccccgccat ttttcaaaaa gatctggacg gtgctgcgga ataattcgaa tcccggcgac
     6121 attatgacct atggacagct tgcccgcgcc gccgggtcgc ctggggcggc ccgcgcggcc
     6181 gggtcttcaa tgcgtgcgaa tccggttccg atcatcgttc catgccacag ggttgtcggc
     6241 gcttcaggcc cgggcggata ctccgccccc ggcggccttg ataccaagag gcatcttctt
     6301 gccatcgagg gggtggccct tcgcctttcc ggcagaacgg gggttcagta atggttgtaa
     6361 ggcacaggcc ggcggcagca tcgaaggcag tccagccggt cgaacagtcc ccattttcgc
     6421 gcatcgtcac cggtcatgac ggcgggctgg acgatcacgt ctttttcgcc acggcggcaa
     6481 gcggcacgga accgtgccac ggttccgtgc cgcttgccgc cgtggcg
//
LOCUS       DAOPPA010000136         6517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_12984_length_6517_cov_4.282266, whole genome shotgun sequence.
ACCESSION   DAOPPA010000136 DAOPPA010000000
VERSION     DAOPPA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12984_length_6517_cov_4.282266"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PKH54_05605"
     CDS             complement(<1..172)
                     /locus_tag="PKH54_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC99399.1"
                     /translation="MAGATQNDDEEMISAINVTPLVDIFLVLLIIFMVTANLFIQQEK
                     KMREIQLTLPTAA"
     gene            complement(150..785)
                     /locus_tag="PKH54_05610"
     CDS             complement(150..785)
                     /locus_tag="PKH54_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC99400.1"
                     /translation="MTFSDMLQEFFIALGVEWVLYLLILLSVLSLAVIFERWLFYFRR
                     RVQPDVARRLVCGSTDGMPADCMEVIVAKTILDGGRNFIDREDAESALETEVRKQKAR
                     YEKGLTFLATLGNNAPFIGLLGTVLGIMAAFFVLADVGETAEKNELLMTAISEALVAT
                     AMGLIVAIPAVMFYNIFRRKVSASVEQTKEIVTGLIHRKFGGGEDGWCDTK"
     gene            960..2132
                     /locus_tag="PKH54_05615"
     CDS             960..2132
                     /locus_tag="PKH54_05615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC99401.1"
                     /translation="MNRIQVAVFAAFLVLVAAGPAWAVKFRFPIIDVERIQFDYVVYA
                     DHDPADGVMTWACLPWCAGTWINRYVECTENFPFCYDAHDGTDFMLIDGFSMMDSGSA
                     QVVAAADGTVIATEDGNYDRCHADIVTQDVTCDGYPMRGNYVKLRHSDGMETWYWHFK
                     KDSIQVEVDQQVKCGDVLGLVGSSGYSSAPHLHFEVRMPTGVGDETAWIDPFAGPHSQ
                     EESYWVLQYDPTTWLPGAYCEGDEIPDFPEPETDQGGTADVPAELAASDEAVSEEVSG
                     DVVEPLDASADGVSDATGPKDVPAVDVGGFDLVVSDLPADAGADGLVADAAPAVDTGL
                     NDNGQVLADSGVGDSVPGEPSGGCSSGPRAVPPFGLLLMVAGAIFAFVSRRRGLFS"
     gene            2129..3169
                     /gene="holA"
                     /locus_tag="PKH54_05620"
     CDS             2129..3169
                     /gene="holA"
                     /locus_tag="PKH54_05620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOC99402.1"
                     /translation="MKIQEIEQIRVELESGKAIPMVYVAGGDDGVMDALVQVFGGHYS
                     AAGTETKVVRLDASGAKSDAWEQLAGIAGSFPMFDEKTIVVMTGCAGGAKVPKETRPF
                     LENPPDHAVFLFCADRKTSASPLAKLVKEHGRVLDFKDLKDAQARAVAISGARQMGLL
                     LDAAAADALIDMVGTDLGAIDSALKSLAGLVVPGAGTVNVGVPDLAGLVRRTRKHAPW
                     DLDEAIEKRDLARAVKVAIRELQDSKDPRSRVIGLFNTVLRRARTILVASDLLADRVG
                     QTEAMERLGITWPFMYERVVDATRRYSRAELKTFLKNAMEMDIRMKRGVAGPETRVVE
                     ILTSLMVRRAGQ"
     gene            complement(3229..3717)
                     /gene="ispF"
                     /locus_tag="PKH54_05625"
     CDS             complement(3229..3717)
                     /gene="ispF"
                     /locus_tag="PKH54_05625"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOC99403.1"
                     /translation="MNAFRVGFGHDRHPFSKPGHGPLALGCVNVDFNRRLLGHSDGDA
                     VAHCIADALLSAAGLPDLGTTFPETCDATTGISGCEILKWTGRLVRDAGWIVGNIDCV
                     VVCDSPRLGHLVPAMCQAAAGALGIGADQISIKPRHAEGLGFAGRGLGIESSAVVLIH
                     RP"
     gene            3863..5830
                     /locus_tag="PKH54_05630"
     CDS             3863..5830
                     /locus_tag="PKH54_05630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOC99404.1"
                     /translation="MFLKLKPSQVFGKNGLAASALPGYEYRAEQERMMAAVQKAIMTD
                     DVIIVEAGTGVGKTLAYLVPAVASGRQTVVATGTRTLMEQLVNKDLAILSGILGEPVD
                     AAVLKGRSNYLCLARLDMAMDSPDLFQPDDRRLLGIIRRWCDETKTGDVGQLSGVAED
                     DAILRSVVCQADTCTGRVCPFCSRCFLFRARARAQEAELVITNHHLLMADLAMSDGSE
                     DDSGAGPLLPSDANLIIDEAHGLEDVATVAFGLVATRAGVAEISRDTLALANHADPRT
                     ARVVVNVARRLDDGFVKLLNLVAGRRERCRLADGPDDVQLSKQAVAAWHGLDQDLEAV
                     GIETTSIAREMERTSEIPGRVAQVRATLCQVLDRDDGSMVRIVERKGHGGSFAAYPVD
                     VSTILRQKMFQKGRPIVLTSATMSVAGQTAFFRSRLGIPEGAPECVLQSPYDYASNVV
                     LYIPSGMPVPTDDNWPGQVIDETVRLIELVGGRTLVLFTSRAALNRAAVELPERVGYP
                     ILVQGTMQRGELVERFRSAGNAVLLATAAFREGLDIAGGALSCVIMDRLPFEPPDEPL
                     LVARSDRMRLSGQEPFFHYQVPLAAIRLRQGFGRLVRRRTDRGVVAILDPRIANRNYG
                     KIFLDSLPPAFRATEFEAVRSWCLRNLLPEN"
     gene            complement(5844..6299)
                     /locus_tag="PKH54_05635"
     CDS             complement(5844..6299)
                     /locus_tag="PKH54_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOC99405.1"
                     /translation="MKRVRYVSRFRENMSPAAIQALAREASEFNSGHQITGALLATGQ
                     IFFQMIEGPRQEIDALWSKIKADPRHVDVVILSSEEGGLSRLCPDWSMKTIDLSIDAS
                     EATLPLRTLIQLIFDQHKIIDSAVEALEKATWNMLLKAELNDMERIAGM"
BASE COUNT         1248 a         1894 c         2054 g         1321 t
ORIGIN      
        1 tggcggctgt cggcagcgta agctgaatct cgcgcatctt cttttcctgc tggatgaaca
       61 ggttcgccgt caccatgaag atgatcagca ggaccaggaa gatatcgacc aggggcgtga
      121 cgtttatcgc ggagatcatc tcctcgtcgt cattttgtgt cgcaccagcc atcctctccg
      181 cctccaaact tcctgtggat caggccggta acgatttcct tggtctgttc gaccgacgcg
      241 gaaaccttgc gcctgaatat gttgtagaac atgactgccg ggatcgcgac gatcaggccc
      301 atggccgtcg cgacaagcgc ttcggaaatg gccgtcatca gcagctcgtt cttttcggcc
      361 gtctcgccga cgtcggcaag gacgaagaac gcggccatga ttcccagaac ggttccaaga
      421 agcccgatga acggggcgtt gttgccaagc gttgccagga aggtaagccc cttctcgtac
      481 cgcgccttct gcttgcgaac ctcggtttca agggcgcttt ccgcgtcttc ccggtcgatg
      541 aagttgcggc cgccgtcaag gatcgtcttc gcgacgatga cttccatgca gtctgccggc
      601 attccatcgg tgctgccgca cacaaggcgc cttgccacgt cgggctgcac ccgtcgcctg
      661 aagtaaaaca gccatcgctc gaaaatgacg gccagcgaga gaaccgaaag caggatcagc
      721 aggtaaagaa cccactcgac cccgagtgcg atgaaaaact cctgaagcat gtccgaaaat
      781 gtcacttttt gaatcccccg ataaaagagg caggtcaagc tggcagtaat aatgtttacc
      841 gaaccccggt ctagtcaagg tcgcatgtga agttatcgat gaaataagcg tttttcccgc
      901 cggacatcgt ataatccggc ctgctgaaac atttgccgcc ggtcggcagg ggggcagtca
      961 tgaatcgaat tcaggtggcc gttttcgcgg cgtttctggt gcttgtcgcc gccgggccgg
     1021 cgtgggcggt caagtttcgt tttccgatta tcgatgttga aagaatccag ttcgactacg
     1081 tggtctatgc ggatcatgac ccggccgatg gcgtgatgac ctgggcctgc ctgccgtggt
     1141 gcgccggcac atggatcaac cggtacgtgg aatgcaccga gaattttcct ttctgttacg
     1201 acgcgcacga cggaaccgac ttcatgctga ttgacggctt ttcgatgatg gattccggaa
     1261 gcgcgcaggt tgtggcggca gccgacggaa ccgtcatcgc gaccgaggac ggcaattacg
     1321 accgctgtca tgccgacatc gtgacccagg acgtgacctg cgacggatac ccgatgcgtg
     1381 gcaactatgt gaaactgcgt cattccgacg gcatggaaac ctggtattgg cacttcaaga
     1441 aggactcgat ccaggttgaa gtcgatcagc aggttaagtg cggcgacgtg ctgggtcttg
     1501 tgggcagctc cggatattcg tcggcccccc atcttcactt cgaggtcagg atgccgaccg
     1561 gggtgggcga cgagacggcc tggattgatc cgtttgccgg cccgcacagc caggaagagt
     1621 catattgggt gcttcaatac gacccgacca catggctgcc aggcgcatat tgtgaaggcg
     1681 atgagatacc ggatttcccc gagcccgaaa ccgaccaggg cggcaccgcg gatgttccgg
     1741 cggagctggc cgcatcggac gaggccgttt ccgaggaagt gtccggcgac gtcgtggaac
     1801 cgttggatgc atccgcggat ggggtctccg acgcgaccgg cccgaaggat gtcccggcag
     1861 tcgatgtcgg cggctttgat cttgttgttt ccgacctgcc ggccgatgcc ggcgcggacg
     1921 gcctggtcgc cgacgctgcc ccagcggtcg atacgggatt gaatgacaac ggccaggttc
     1981 tggccgattc tggcgtgggc gacagcgtgc cgggtgaacc ttccggcggc tgcagttccg
     2041 ggccgagggc cgttccacct ttcggcttgc tgttgatggt cgcgggcgct atctttgcct
     2101 tcgtgtcgcg cagaaggggc ctgttcagtt gaagatccag gaaatcgaac agattcgcgt
     2161 cgagcttgaa tcgggaaaag cgattcccat ggtctatgtc gcgggtggcg atgacggcgt
     2221 gatggacgcc cttgtccagg tctttggcgg ccactattca gccgccggga ctgaaaccaa
     2281 ggtggtccgg ctggacgctt cgggcgccaa gtccgatgcg tgggaacagc ttgccgggat
     2341 tgccggctcg ttcccgatgt tcgacgaaaa gaccatcgtc gtgatgaccg ggtgcgccgg
     2401 cggcgcgaag gtgccgaagg aaacgcgtcc attcctggaa aatccgcccg atcatgccgt
     2461 gttcctgttc tgtgccgacc gaaagacttc cgcgtcgccg ctggcgaagc tggtcaagga
     2521 acacggccgg gttctggatt tcaaggatct gaaggatgcc caggcccgcg cggtcgcgat
     2581 ttccggcgcc cggcagatgg ggttgctgct tgatgcggcg gccgccgacg cgctgattga
     2641 catggtcggc accgatctcg gggcaatcga cagcgccttg aagagccttg ccggtcttgt
     2701 ggtaccgggg gccggaacgg tcaatgtcgg cgtccccgat cttgccggcc tggtgcggcg
     2761 tacgcgcaaa catgccccgt gggatctgga cgaagcaatc gaaaagcggg atctggcaag
     2821 ggcggtcaag gtcgccatcc gggaactgca ggattccaag gatcccagat cgcgtgtcat
     2881 cggacttttc aacacggtcc tgcgaagggc caggacgata ctggttgctt cggatcttct
     2941 tgccgacagg gtcgggcaga ccgaggcgat ggaaaggctg ggaatcacct ggcccttcat
     3001 gtacgaaagg gtggtcgatg caaccaggcg ctattcccgc gccgaactga agacgttcct
     3061 gaagaacgcg atggaaatgg acatccgcat gaagcgcggc gtcgcgggtc ccgaaaccag
     3121 ggtcgtcgag atcctgactt ccctgatggt caggcgcgcc gggcagtgac cttcaagcgt
     3181 tttcggcgaa attgacgaag actgatgggc cggatcccgg ggccgcgcct acggacggtg
     3241 gatcaggact acggcgctgg attctatacc caggccgcgg cccgcaaatc ccaggccttc
     3301 cgcatggcga ggcttgattg aaatctggtc cgcgccgatt ccgagtgccc cggcagcggc
     3361 ctggcacatc gccggaacca ggtggccaag ccgtggactg tcgcagacga ccacgcaatc
     3421 aatgttgccg acgatccagc cagcatcgcg aaccagcctt ccggtccatt tcaggatctc
     3481 gcagccggaa attccggtgg tggcgtcgca agtctcggga aatgtcgtcc cgaggtcggg
     3541 caacccggcc gccgaaagca gcgcgtccgc gatgcagtgc gcaaccgcgt cgccgtccga
     3601 atgaccgagc agccgccggt tgaagtccac gttcacgcag ccaagcgcca gcgggccgtg
     3661 tcccggtttt gaaaacggat gcctgtcatg accaaaacca accctgaacg cgttcatgat
     3721 gcaccgccat tattccgcgc cgaaacggcc cggaatgtgt tgtccgaatt ctgtctataa
     3781 atcggcaagg ccccggaatg caaatgtcgc gtcgaatttt tccgtgctat cttgcgcgcg
     3841 gccatgtttt tcggcgccgt cgatgttcct gaaattgaag ccatcccagg tgttcgggaa
     3901 gaatgggctg gccgcgtcag ccctgccggg atacgagtac cgtgccgagc aggaaaggat
     3961 gatggccgcg gtccagaagg ccatcatgac cgacgacgtg ataatcgtcg aggccggcac
     4021 cggcgtggga aagacgctag cttacctggt tcccgccgtt gcgtccgggc gccagacggt
     4081 cgtggcgacc gggacccgca cattgatgga acagcttgtc aacaaggatc tggcgattct
     4141 ttccgggatt ctcggggagc cggtcgatgc cgcggtcctg aagggcagat cgaattatct
     4201 gtgcctggcc cgtctggaca tggcgatgga cagcccggat cttttccagc ccgacgatcg
     4261 caggcttctg ggaattattc gcaggtggtg cgacgagacg aagaccggcg atgtcggcca
     4321 gctttccggc gttgccgaag acgatgcgat cctgcgttcg gtcgtctgcc aggccgacac
     4381 gtgcaccggc cgggtatgcc cgttttgttc acgctgcttc ctgttcaggg cccgggcccg
     4441 cgcacaggag gccgagctgg taatcaccaa tcaccatctc ctgatggccg atctggcgat
     4501 gtcggacgga agcgaggatg attcgggcgc cgggcccctt cttccttcgg acgccaacct
     4561 gataatcgac gaggcgcatg ggcttgaaga cgtggcaacc gtggctttcg gcctggtcgc
     4621 gacgcgtgca ggtgtcgcgg agatttcccg ggatacgctt gcactcgcga atcatgccga
     4681 tccgcggacc gcccgggttg tggtcaacgt cgcgcgcaga ctggatgatg gtttcgtcaa
     4741 actgctgaac ctggtggccg ggcggcgcga gcggtgcagg ctggcggacg ggccggacga
     4801 cgtgcagctt tcgaagcagg ccgtggctgc atggcacggg cttgaccagg atctcgaagc
     4861 ggtcggcatc gaaaccacct cgatcgccag ggaaatggaa agaaccagtg aaattcccgg
     4921 tcgcgtcgcc caggttcgcg cgaccctttg ccaggtactt gatcgcgacg acggttcgat
     4981 ggtcaggatc gtcgaaagaa agggccatgg cggctcattc gccgcatacc cggtcgatgt
     5041 ttccacgatt ctgcgccaga agatgttcca gaagggacgg ccgatcgtcc tgacaagcgc
     5101 gacgatgagc gtggccggcc agaccgcttt cttccgcagc cggctgggaa ttcccgaagg
     5161 tgcgccggag tgcgtgctcc agtcgccgta cgactatgcg tcgaatgtcg tgctttacat
     5221 tccgtccggg atgccggttc caaccgatga caactggccc ggccaggtca ttgatgaaac
     5281 ggtgaggctg atcgaactgg tcggcggacg tacgctggtg cttttcactt cacgcgctgc
     5341 cctgaatcgc gccgccgttg aactgcccga acgggtcggt tatcccatcc tggtccaggg
     5401 gactatgcag cgtggtgaac tggtcgaacg cttccgttcg gccgggaacg ccgtacttct
     5461 ggcgaccgcg gcatttcggg agggactcga cattgcaggc ggggccttga gttgcgtgat
     5521 catggacagg cttccgttcg aaccgcccga cgaaccactt ctggtggccc gctcggacag
     5581 gatgagattg tcggggcagg aaccgttttt ccattaccag gttccgctgg cggcgatccg
     5641 gcttcggcag gggtttggca gactggtgcg ccgtcgcacc gacaggggcg tggtcgcgat
     5701 actcgatccg cgaatcgcca acaggaatta cggcaaaatt ttcctcgaca gcctgccgcc
     5761 ggcctttcgc gcgacggaat tcgaggcggt tcgatcctgg tgtcttcgga atcttttacc
     5821 agagaattga ggataacggc ggatcacatg ccggcgattc gttccatgtc gttcaattcg
     5881 gccttcagca gcatgttcca cgtcgccttt tcaagcgcct cgacagcgct gtcgatgatc
     5941 ttgtgctgat caaagataag ctgaataagg gtgcgaagcg gcagggtagc ctcggacgca
     6001 tcaattgaaa gatcgatagt cttcatcgac cagtccgggc acagacggct cagcccgcct
     6061 tcctcggaag acaggatgac cacatcgaca tgccttggat ccgccttgat cttcgaccac
     6121 agcgcgtcga tctcctggcg cggaccttcg atcatctgaa agaagatctg gccggtcgca
     6181 agaagcgccc cggtgatctg gtgtcccgaa ttgaactcgg acgcctcccg ggcaagggcc
     6241 tggattgccg ctggcgacat gttctcgcga aacctcgaaa catagcgtac gcgtttcatc
     6301 tggattcccc tgattgattg aatagttggg tctgaccaca aagtcttgcc ccgatcctgc
     6361 cggccggtca agttccgcgc tcattttcca gcgaaaacgc gaggttttgc agttatttcg
     6421 gcgtttcagc cagatccagc ggggcgtacg gggccgcggc ggttctttca agcacgccgg
     6481 cgtaatcttc gtccaccgtg aagaattcgg tgttcag
//
LOCUS       DAOPPA010000137         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13044_length_6494_cov_3.682093, whole genome shotgun sequence.
ACCESSION   DAOPPA010000137 DAOPPA010000000
VERSION     DAOPPA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13044_length_6494_cov_3.682093"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKH54_05640"
     CDS             complement(<1..583)
                     /locus_tag="PKH54_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC99406.1"
                     /translation="MTVPLPNPELEQQIGERVFKVADGFFRIPLPTGYRIGDVNVWLL
                     DGDEPVLFDTGTGSQESLQALDDALAVVGRRVKDIRHLLMTHAHIDHCGGAGRIAEAS
                     GCAVHVHAREVDRVRDFVKASRAELFPLIPMMMQMGFDRSVSQHALLMLDKLGASATP
                     CPSAGELPKSLQTGRGTITWTYVPGHSAADVLFR"
     gene            complement(613..3849)
                     /locus_tag="PKH54_05645"
     CDS             complement(613..3849)
                     /locus_tag="PKH54_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99407.1"
                     /translation="MKSSNKLTLAAALAFAAIAVGCSGGDNGTDIVDGDVAGDVSQDV
                     GSDIGNEVAADAADDVPVVKTGYSAGWGVTDVSGVTGTMMGGYGFCMNAPDSCRYAEG
                     VHDPLLARAIAVRDNASGEAVILISVDVVGILRYDIDLIHAAAPARFKEAFGIDGFDG
                     RRIVMTASHTHHSTDTMGLWGPLDGSGRDEEYATHMREQVLEAAVAAWADLKPVNIFY
                     TGTGSAPNYDKDKWSDDQVIQTLRFADRETGKTRFTLTRWSAHVTNYPQQLQAFSAGH
                     HGTFSKAMEELTGAPAALFQGAIGSVYSEGYMFDEPGQPACEMDDMFADGYQDPDLRP
                     EFISAVTCMGYGLAKRAFAAIQDEVAVPETGVKNRFHKFAFHPETKYPVMFDWTMFSY
                     VEMGPLHFDVPPADQIDNPESTMDTQFNWVTIGDVNLITTPGEAFPLFADQIRHVLSR
                     SSDKNFVLGLGQDWLGYIPAWQQWQVEDDAIVYNKALAGGQYLPKRYLEELQTLVTTE
                     TGLPADCTGDIKKCSDDGNQIMECVGGIWIVNTDCFAGFGQICDDTLDGGDGIGCVNR
                     WAWHQPVWSTCEDNLLATPESLVSKAAYYDDIAMRLHFHPQLKWIAPVTLPKSEVECP
                     QDVAGPCYAPTVPEATATWEDVEYWHTGENDGLWSALYLASQAFRYGATKDPVALENI
                     RKLMEGEKWRMEITGVPGLFTRQYIPKDVAGIQCPTDLHEYIPDDIKDDNKWLRVGDD
                     GCIYTVDGGTMEWVKQEVCGLDNYAGWCLLDNVSQDEYAGHMFALGAIYKLVDDAAIK
                     ADAAKLLGQVGRHLADNWMHFIDWDGRDTEHGKMNIYSFADSPGFLAVHSLGFMLMAA
                     EATNNQRLWDFFNKCLLQKDGGPCEGWPYPEPPYDTEISNMLLYNGPLGCKANYNNFS
                     MVFAAIHNLAWFLKDAGLMAIVQDTFENHFMDDGNQPRALTVQKNPWFNFGFAAMKKL
                     GPENPQAFDLVEDGICSLREFQASQAQRFGNSAENYAEFCESRFEGRFMAENPIPVAE
                     RCTATFEWWGDPYQLETCTAAPWNIQVPTGYLLPYWMGRYYGFISQDI"
     gene            3998..5263
                     /gene="pepT"
                     /locus_tag="PKH54_05650"
     CDS             3998..5263
                     /gene="pepT"
                     /locus_tag="PKH54_05650"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOC99408.1"
                     /translation="MKIQFTATEKAELLERFLRYTRIDTQSDENSQASPSTEKQKDLA
                     RLLVSELKALGLGDAAMDQWGYVTATIPASNAELDRSVPVLGFLAHMDTAFGCSGKDV
                     KAQVIENYDGRDMVLPGNPEVVIRNADNPNLQRVIGHTLITTDGTTLLGADDKAGIAE
                     IMTAAHWMMKHPEFQHGRIRIGFTTDEEVGRGTEHFDVKAFGADVGYTFDGGDLGEIE
                     DETFCADFAKVTFTGKDVHPGYAKDKMINAVRVATFFVGQLPVTNLPETTEKREPYLH
                     PYDIHGDVSKVELKVLLRAFTVAELDERADVLAAAAVATEGAFPGCSVVVERREQYRN
                     MGMKLAERPEVMANLDKAVRLQGIEPVRKAIRGGTDGARLSFMGLPTPNVWAGGQNFH
                     SLQEWASLDWMVAASETAVRIAAVWAGRE"
     gene            5294..>6494
                     /locus_tag="PKH54_05655"
     CDS             5294..>6494
                     /locus_tag="PKH54_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99409.1"
                     /translation="MKNVINVLFVVAVCQIFAVGCCCRKDGSCGRAAEAAACGTTSGA
                     DAAQGIQAASPAANSENQMIFITDEAIAGTVAKLVEKHGEAARARIEKGVRQVAAYWN
                     QKDGSVEEFESFCMEQFIADPAALTMLLDRFRVAMESISGHAVAMTRKLREPVDLATF
                     QTLPVDLLFATLNPFDHLSEDFFTTKVAFVGLLNFPLLDTSEPLEFSRREWAEARLAQ
                     TFARRIPGEVALGATRAYAAAEDYIARYNIHMGGVQNQDGSTPFPADLKLISHWGLRD
                     HIKGMYSDPVGNLPNQQTILQIMNRIVNQEIPAAVVDAPGLTWNPWTNELKDAEGKAV
                     DAAREPDTRYARILEVFRAEQQIDKYSPLYPTLIDRRFKLDREIREERVRRMLETVIS
                     SPTTKKVA"
BASE COUNT         1256 a         1943 c         1991 g         1304 t
ORIGIN      
        1 tccggaaaag cacgtcggcg gcggaatgcc ccgggacata agtccatgtg atggtgccgc
       61 gccccgtttg aagcgacttc ggcaattccc cggctgaagg gcagggcgtg gccgacgccc
      121 ccagcttatc cagcatcaga agtgcgtgct ggctgacgga ccggtcgaac cccatctgca
      181 tcatcattgg aatcaacgga aacagttccg ccctggatgc cttgacgaaa tcgcggaccc
      241 gatcgacttc acgcgcgtga acatgtaccg cgcagccgga cgcttccgca atccggccgg
      301 ccccaccgca gtggtcgatg tgcgcatggg tcatcagcag gtggcggatg tccttcacgc
      361 gtcgcccgac cacggccagt gcgtcgtcaa gggcctgcag gctttcctga ctgccggttc
      421 cggtgtcgaa aagcaccggc tcgtcgccgt caaggagcca gacattcacg tcgccgatgc
      481 ggtaaccggt cggcagtgga atccggaaaa atccgtcggc gactttgaaa acgcgctcgc
      541 cgatttgctg ctcgagttcc gggtttggaa ggggaaccgt catttcatga aatcccggtt
      601 tgggggcggc gatcagatgt cctgggaaat gaagccgtaa tagcgaccca tccagtaggg
      661 cagcaggtat ccggttggca cctgtatgtt ccacggggcg gcggtgcacg tctcaagctg
      721 gtagggatcg ccccaccact cgaacgtcgc ggtgcatcgc tcggccactg gaatcgggtt
      781 ttccgccatg aagcggccct cgaatcgcga ctcgcagaat tcggcgtagt tttctgcgga
      841 attcccgaat ctctgcgcct ggctggcctg gaactcacgc aggctgcaaa tgccgtcctc
      901 gacaaggtcg aatgcctgcg ggttttccgg gcccagcttc ttcatcgccg caaacccgaa
      961 attgaaccac gggttcttct ggacggtcag cgcgcgcggc tggttgccgt cgtccatgaa
     1021 atggttttcg aaagtgtcct ggacgatcgc catcaggccg gcatccttca ggaaccacgc
     1081 caggttgtga atcgccgcga aaaccatgct gaaattgttg tagttggcct tgcatccaag
     1141 cgggccgttg tacagcagca tgttggaaat ctcggtgtcg tacggcggtt ccgggtacgg
     1201 ccagccttca caggggccgc cgtccttctg aagcaggcac ttgttgaaga aatcccacag
     1261 cctctggttg ttggtcgcct cggcggccat cagcatgaat cccagcgaat gaaccgccag
     1321 gaatccgggg ctgtcggcga acgaatagat gttcatcttg ccgtgctcgg tgtcgcggcc
     1381 gtcccagtcg atgaagtgca tccagttgtc cgcaagatgc ctgccgacct gtccgagcag
     1441 ctttgccgcg tcagccttga ttgccgcgtc gtcaaccagc ttgtatatgg cgcccagcgc
     1501 gaacatgtgg ccggcgtatt catcctggct gacgttgtcc agcaggcacc atccggcgta
     1561 gttatcaagc ccgcagactt cctgcttgac ccattccatc gtgccgccat cgacggtata
     1621 gatgcacccg tcgtcgccca cgcgcagcca cttgttgtcg tccttgatat cgtccgggat
     1681 gtattcgtgc aggtcggtcg ggcactggat tccggcaacg tccttcggaa tgtactggcg
     1741 cgtgaacagt cccggaacgc ccgtgatttc catgcgccac ttttcgcctt ccatcagctt
     1801 gcggatgttt tccagggcga cgggatcctt cgtggcgccg taccggaatg cctgggacgc
     1861 caggtaaagt gcgctccaca gaccgtcgtt ttcaccggta tgccagtatt cgacatcttc
     1921 ccaggttgcg gtcgcctcgg gcacggtcgg ggcgtaacac ggccccgcaa catcctgcgg
     1981 gcattcgact tccgatttcg gaagggttac cggcgcaatc cacttaagtt gcgggtgaaa
     2041 gtgcagccgc atcgcgatgt cgtcgtaata cgcggccttc gaaaccaggg attccggggt
     2101 cgccagcaaa ttgtcctcgc acgttgacca caccggctgg tgccatgccc agcggttgac
     2161 acagccgatg ccgtcgccgc cgtcaagcgt gtcgtcgcag atctggccga agccggcaaa
     2221 gcagtcggtg ttcacaatcc agatgccgcc gacgcattcc attatctggt tgccgtcgtc
     2281 gctgcacttc ttgatatcgc ccgtgcagtc ggcgggcagg cccgtctccg tcgtaaccag
     2341 agtctggagt tcctcgagat aacgcttcgg aaggtactga ccgcccgcaa gcgccttgtt
     2401 gtaaacgatg gcgtcgtcct cgacctgcca ctgctgccac gccgggatat atccaagcca
     2461 gtcctggccc agtccgagaa cgaaattctt gtcgctggat ctggaaagca cgtggcggat
     2521 ctggtcggca aacagcggga atgcctcgcc gggggtcgta atcaggttca cgtccccaat
     2581 ggtgacccag ttgaactggg tgtccatcgt ggattccggg ttgtcaatct gatcggccgg
     2641 cggtacatcg aagtgaagcg ggcccatttc gacgtaactg aacatggtcc agtcgaacat
     2701 gaccgggtat ttcgtttcgg ggtggaacgc gaacttgtgg aaacggttct tcacgccggt
     2761 ttccggaacg gcgacttcgt cctggattgc cgcgaaggca cgctttgcaa ggccgtaacc
     2821 catgcacgtg acggcgctga tgaactcggg ccgaaggtcc ggatcctggt acccgtcggc
     2881 gaacatgtcg tccatctcgc aggcgggctg gccaggctcg tcgaacatgt atccttcgct
     2941 atagaccgat ccgatggcgc cctggaaaag cgcggccggg gcgccggtca gttcttccat
     3001 tgccttgctg aaggtgccgt ggtggccggc cgagaacgcc tggagctgct gcgggtaatt
     3061 tgtgacgtgg gccgaccagc gcgtaagcgt gaacctggtc ttgcccgttt cgcggtcggc
     3121 gaagcgcagc gtctggatga cctggtcgtc ggaccacttg tccttgtcgt agttcggcgc
     3181 cgagccggtg ccggtgtaga agatgttgac cggcttcagg tcggcccacg cggccacggc
     3241 ggcctcgagc acctgctcgc gcatgtgtgt cgcatattcc tcgtcgcgtc cggaaccgtc
     3301 cagcgggccc caaaggccca tcgtgtcggt cgaatggtgc gtgtgggaag ccgtcatcac
     3361 aatgcgtcgc ccgtcaaagc catcaatgcc gaaagcttcc ttgaacctgg ccggggccgc
     3421 ggcgtgaatc aggtcgatgt cgtaacgcag gatgccgacc acatcgacgg aaatcaggat
     3481 caccgcttcg ccgctggcgt tgtcgcgcac tgcaatggcc ctggcaagca gtgggtcgtg
     3541 cacgccctcg gcgtatcggc aggaatcggg ggcgttcatg cagaagccgt atccgcccat
     3601 catggtgccg gtaacgccgc tgacgtcggt cacgccccag ccggcggaat atcccgtctt
     3661 cacgacagga acgtcgtccg cggcgtccgc ggcgacttca ttgccgatgt cgctgccgac
     3721 gtcctgtgaa acgtcgccgg caacgtcgcc atcgacgatg tcggtgccgt tatcgccgcc
     3781 ggagcatccg accgcaatcg ccgcgaaagc aagcgcggct gccagtgtaa gtttgtttga
     3841 agacttcatc ggttctgcct cgttcgtgtt ctggccgccg tgtccagtgt ccgccgcggc
     3901 cgggacggtt ggcagagatt gtagtctatt taagtgttcc ggtcgcaaac aattgaaggt
     3961 aaactgggcg ccgtcttgaa actgggaagc gtcaatcatg aagatccagt tcaccgccac
     4021 ggaaaaagca gaattacttg aaagatttct tcgctatacg cggattgaca cgcagtcgga
     4081 cgagaattca caggccagcc catcgacgga aaagcagaag gatctggccc gcctgctggt
     4141 ctcggaactg aaggcactcg ggcttggcga tgccgccatg gatcagtggg gatacgtgac
     4201 ggcgaccatt ccggcctcga atgccgaact tgaccgctcg gtgcccgtcc ttggctttct
     4261 tgcacacatg gacaccgcct tcggttgcag cggaaaggac gtcaaggccc aggtgattga
     4321 aaactatgac gggcgcgaca tggttcttcc cggcaatccc gaagtggtca tccgcaatgc
     4381 cgataatccg aatctgcagc gcgtcatcgg ccatacgctg attacgaccg acggcaccac
     4441 gctgcttggc gccgacgaca aggccggaat cgccgaaatc atgaccgccg cgcactggat
     4501 gatgaagcat cctgaattcc agcacggtcg cattcgaatc ggcttcacca ccgacgagga
     4561 agtcggtcgc ggcaccgagc atttcgatgt caaggcattc ggcgccgatg tcgggtacac
     4621 gttcgacggc ggcgatcttg gcgaaatcga ggacgaaacg ttctgcgccg acttcgcgaa
     4681 ggtcactttc accggcaagg acgtgcaccc gggatacgcc aaagacaaga tgatcaacgc
     4741 ggtccgcgtg gcgacgttct ttgtcggcca gcttccggtt accaaccttc cggaaacgac
     4801 cgaaaagcgc gaaccttacc ttcatccata cgacatccac ggcgacgttt cgaaggttga
     4861 actgaaggtt ctgctgcgtg ccttcactgt ggctgaactg gatgaaaggg ccgatgtgct
     4921 ggccgccgcg gccgtggcca ccgaaggcgc gtttcccgga tgttccgtgg tcgtcgaaag
     4981 gcgggagcag taccggaaca tggggatgaa gctggcggaa cgtcccgaag taatggcaaa
     5041 tcttgacaag gccgtcaggc ttcagggaat cgagccggtt cgcaaggcga ttcgcggcgg
     5101 caccgatggc gcaaggctgt ccttcatggg acttccgacc ccgaacgtat gggccggcgg
     5161 gcagaatttt cacagcctgc aggaatgggc gtcacttgac tggatggtgg ccgcatcgga
     5221 aacggccgtc aggattgccg cggtctgggc cggaagggaa taatccgccc gtcgcccgcc
     5281 tctggaggtt ttcgtgaaga acgtcatcaa cgtgctgttc gttgtcgcgg tttgccagat
     5341 cttcgccgtg ggttgctgct gcaggaagga cggttcgtgc ggtcgcgcgg ccgaagcggc
     5401 ggcatgcgga accacatccg gcgccgatgc tgcccagggc atccaggccg cgtccccggc
     5461 agccaattcg gagaaccaga tgatttttat taccgatgaa gcgattgccg gcacggtggc
     5521 aaagctggtc gagaagcacg gcgaggcggc cagggcacgg attgaaaagg gcgtgcgcca
     5581 ggtcgccgcg tactggaacc agaaggatgg ctcggtcgag gaattcgagt cattctgcat
     5641 ggaacagttc atcgccgatc ccgccgcgct gaccatgctg ctggatcgtt tccgggtcgc
     5701 gatggaatcc atcagcggcc acgcggtggc aatgacccgc aagcttcgcg aaccggtcga
     5761 tctggccaca ttccagacgc tgccggtcga tctgctgttt gccacgctga acccgttcga
     5821 ccatctgtcc gaggacttct tcacaaccaa ggtcgcgttt gtcggcctgc tcaattttcc
     5881 ccttctggat acaagtgaac cgcttgagtt ttcgcgacgc gaatgggccg aggcaaggct
     5941 tgcgcagacg tttgcccggc gcattcccgg cgaggtcgcg cttggtgcga cccgggccta
     6001 tgccgccgcc gaggattaca tcgccaggta taacatccat atgggcggcg tccagaacca
     6061 ggacggcagc acccccttcc cggcggatct gaagctgatt tcccattggg ggcttcgcga
     6121 tcacatcaag ggaatgtatt cggatccggt gggtaacctg ccgaaccagc agaccatcct
     6181 tcagatcatg aaccgcatcg tcaaccagga aataccggca gcggtcgttg acgcacccgg
     6241 actcacctgg aatccatgga cgaacgaatt gaaggacgct gaaggcaagg ctgtggatgc
     6301 cgcccgcgag ccggacacca ggtatgcaag aatcctggaa gtctttcgtg ccgagcagca
     6361 gattgacaaa tacagccccc tttatccgac gttgatcgac cgtcggttca agctggatcg
     6421 tgagattcgt gaagagcgcg tgcgccggat gcttgaaacc gtcatttcgt cgccgacgac
     6481 gaagaaggtc gctg
//
LOCUS       DAOPPA010000138         6470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13096_length_6470_cov_3.939673, whole genome shotgun sequence.
ACCESSION   DAOPPA010000138 DAOPPA010000000
VERSION     DAOPPA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13096_length_6470_cov_3.939673"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKH54_05660"
     CDS             complement(<1..922)
                     /locus_tag="PKH54_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC99410.1"
                     /translation="MVATAFLNLQAGGLIGFAVGSASAAEGAGARKLAVKVYPKTAGD
                     EALAKAVYVVLRGGADRLAQQGFQRATGMPPAAAQALARAWQNVDKGRALLKENKIAD
                     AFAAFTEAENSARAYMGYADRALVGHIYKGLGIGFIAASKPAQAKRAIKLSLFAYPNQ
                     TKSEYSYSKEFENFILSAQREIMDSPKGMMEVETSPEGAEVYVDFEFKGYSPVTVQNL
                     VVGEHIVTFMADGYEPLSQLVDITGGPAEYAMGELIELDNAGAVQKASDELGAAIGTQ
                     SVGPKAGQYATLVGATDLLAVSFGASGDNFL"
     gene            complement(1007..2998)
                     /locus_tag="PKH54_05665"
     CDS             complement(1007..2998)
                     /locus_tag="PKH54_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOC99411.1"
                     /translation="MKKSILLCFAMIPVLALTACGPVDGAEEGTGVGFLGMNVSAATN
                     SCSGAPAPTPLDQIDRIRVLVNNLTVKDRNGVDVPICKCSKVDVEEGVAEIDGIREGQ
                     GRVVTVLGYSGGNSNPSWFGRARNVNIVEDRASDIDMVLSRYDSFTCISQQYADGVTE
                     ADKGAQKFVHAAFPAAVRLGDGRVFVSGGLTGKVSRGEVDGAQEYELNSPTDKAFIYD
                     PASGVFEFVGNMKVARAGHAMAYVAIGGRDSVVIFGGTDKVIMRTGGNFPFSFDSESA
                     FNSIEIFDVKDGTFTIPLHEGGELNGQPKTMELRRAFLNVGRMGDNSVLIVGGGEWPV
                     DETPTPDGQQTIDYRKAEIWAPYADGGKGGMLAVSGSLAMSSQHNGGTMIRLPDTGRG
                     LSRFLIVGGSTNRESVIEVFTQSSKQEEGVPGVFKIRSDAEFASLNYDKTLPKMFFPS
                     VVQIMPDPADIAHERFLITYGAFESSKQVKADAYKIGSSTQMAYELRLYEDTSNVSKV
                     DQKDGVRMVPIVGDCARRAMGAMFVSHDRKSVVMFGGVAGNSGVSNLGTCKFDATRTT
                     FDKTDAFEKLPAGDRSFLSRAGFAAVSLPDDTIFLAGGMISLDTLDGAGSGMIEVYTP
                     EMLWTPEMYPECMTGGAPETLTEGMRNDLLSELKECWRD"
     gene            complement(3194..5677)
                     /locus_tag="PKH54_05670"
     CDS             complement(3194..5677)
                     /locus_tag="PKH54_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99412.1"
                     /translation="MHRLSWIFAALIAVVVIGCGGGNTTPDFGGGEDVNYPDNGGDQG
                     QEDVVADVPEVGPDLNPGDTKEVSEDTGPVPCQKNSDCVDAPVELGQCQKKVCGEENF
                     CVAGWDSACCMETVYFHEDFEGDLSGWTINDPRPNDFVGWQIAANRRAFGSKSAYFGN
                     PTCLTYYSGALDANCEPVDPEQKDSEAVRASMTSTKFFLPPISSAEATFIASAYIWVE
                     SEPMIGGIPKEVQPDQFRMSIVTTAGETDVTELVVSSTIVDKNSNGVFVYISGNVSSA
                     VGKEAALRLSFDSLEGSNNLFEGVYVDEVKVFSICSAQCDSGTECTADEEACTDDKCQ
                     VFGNREQKGACTHPIMPACVEPVCTPQNVATKCPSSDPCVSVSCPAGACVYTELPEDQ
                     CCRQFELLSTGFEDGTTGGFELWAYMDNETVKWQPSSFKSATGAWALYYGNTVTRTYD
                     SGAFMNNGEASSQELELRAGYNGFLSFDLMLSTEWDNVTPANYYNPAQWDLFTVQVVE
                     RMGQPTEAVTDVWSSHNIHGTTGGVFIPVGVDLSPWAGKVVRVRFRFETGNEEFNNFE
                     GVYIDNVKVIDDKCKARECMANTDCGVDGVCRVGGCVDNVCDVQLVGATGCCAVQQDC
                     NDNDDCTSDGCVANQCMHEFIEKPGCCFEDVRADYTFDIFGDLDGFAVVNDSVPGSGG
                     ADVRWHLSDRRFHGGTRAMYFGNGDNANFDNGGIARGKATSPEFRVPATGDYELSFAV
                     FVDVQTDSAKDKFVVDVMDGLTPTTVFSKAAVPSAVYRNWFEVTGINLNTFKGKNIRL
                     RFSFDSVDALENTGEGIFVDSIRVVRACD"
     gene            5874..>6470
                     /gene="pilM"
                     /locus_tag="PKH54_05675"
     CDS             5874..>6470
                     /gene="pilM"
                     /locus_tag="PKH54_05675"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOC99413.1"
                     /translation="MGLFGQHGGLVGLDIGSSSIKLVRLKQSAGSWALQAFSLGHLPP
                     DAIIDGRIMNFSVVIERLRELMREAGIKGGSCAIAISGASLIVKRLSLPEMTREELAE
                     SITWEAEQYIPFDIKDVNVDAQIINSRAGQGQMDVMMVAAKKDVVNEYVSVATEAGLV
                     TEVVDVAIFAALNMFETNYTVPADQTIALLNIGANTINV"
BASE COUNT         1384 a         2008 c         1797 g         1281 t
ORIGIN      
        1 ccaggaaatt gtcgccggaa gcgccgaaag acactgccag cagatcggtc gcgccaacga
       61 gcgtcgcgta ctggcccgcc ttcggtccga ccgactgcgt gccgattgcc gcgccgagct
      121 catcggaagc cttctgaacc gcaccggcat tatccagttc aatcagttcg cccatcgcat
      181 actcggccgg tccgccggtg atatcgacaa gctgcgaaag cggctcgtat ccatcggcca
      241 tgaaggtgac gatgtgctcg ccaacgacca ggttctgaac cgtcaccggc gaatagccct
      301 tgaactcgaa gtcaacatac acttcggcgc cctcgggcga ggtctcaact tccatcatgc
      361 ccttcgggga gtccatgatc tcgcgctggg ccgaaaggat gaaattttca aattccttcg
      421 agtacgagta ctcggacttg gtctggttag gatacgcgaa cagggacagt ttgatagccc
      481 tctttgcctg ggcgggcttc gatgccgcga taaatccgat accaaggccc ttgtagatgt
      541 gtccgaccag ggcgcgatcc gcgtatccca tgtacgcacg tgccgagttc tcggcctcgg
      601 taaaggccgc gaatgcgtcg gcgatcttgt tttccttcag tagtgcccgc cccttgtcaa
      661 cattctgcca cgccctggca agagcctgtg ccgcggccgg aggcatgccc gtcgccctct
      721 ggaagccctg ctgggcaagc cgatcggcgc caccgcgcag gacgacataa actgcctttg
      781 ccagcgcctc gtcaccggcc gtcttgggat aaaccttgac agcaagcttg cgcgccccgg
      841 caccctcggc agccgacgcc gaaccgactg caaacccaat caggccaccc gcctgcaaat
      901 tcaggaacgc cgtcgcgacc aagacagata caacccgaag aagccgtttt gaccggcaca
      961 tcggcgaaat caacgatacc cttctgaatg ggctggtatt catcaatcaa tccctccagc
     1021 attctttcag ttcactgagc agatcattac gcattccctc tgtcagggtc tcgggcgccc
     1081 cgccggtcat gcattcgggg tacatctcgg gcgtccacag catctcgggg gtatacacct
     1141 cgatcatgcc ggacccagcc ccgtcaaggg tatccaggga aatcatgccg cccgccagga
     1201 aaatggtatc gtcgggaagc gaaacggccg cgaaaccggc gcgggacagg aacgaccggt
     1261 cacccgccgg caacttctca aacgcgtcgg tcttgtcgaa ggtcgtcctg gttgcgtcaa
     1321 acttgcacgt gccaaggtta gacacaccgg aatttccggc gacgccgccg aacatcacaa
     1381 cggacttgcg atcgtggctg acgaacatgg cgcccatcgc ccgccgcgca caatccccga
     1441 caataggaac catgcgcacg ccgtccttct gatccacctt gctgacgttg gatgtgtcct
     1501 cgtaaagccg aagctcgtac gccatctgcg tcgatgaacc gatcttgtag gcatccgcct
     1561 tgacctgctt cgaagactcg aacgcgccgt aagtaatcag gaaacgctcg tgcgcgatat
     1621 cggccggatc gggcatgatc tgcacgaccg aagggaagaa catcttcggc agggtcttgt
     1681 cgtagttcag gctggcaaat tcagcgtccg agcgaatctt gaacactccg gggacgccct
     1741 cttcctgctt tgacgactgg gtgaaaactt cgatgacgct ctcacggttc gtgcttccac
     1801 cgacgatcag gaagcgggaa aggccgcgtc cggtatcggg aagacgaatc atcgtcccgc
     1861 cattgtgctg ggaactcatc gccagcgaac cgcttaccgc cagcattcca ccctttccgc
     1921 catcggcgta gggtgcccag atttcggcct tgcggtaatc aatcgtctgt tgaccgtcgg
     1981 gagtcggggt ttcatcgacc ggccattcac cgccgcctac aatcagaacc gagttgtcgc
     2041 ccatccggcc cacattcagg aacgcccttc gaagctccat cgtcttgggc tgaccgttca
     2101 gttcgccacc ctcatgaagc ggaatcgtga aggttccgtc cttcacgtca aaaatctcga
     2161 ttgagttaaa cgcggattcc gagtcgaagg aaaacgggaa attcccgcca gtccgcatga
     2221 ttaccttgtc ggtgccgccg aaaatcacca cggaatcgcg accgccgatg gcgacgtatg
     2281 ccatggcgtg tcctgcgcgg gccaccttca tgttgccgac gaactcgaaa acaccggaag
     2341 ccgggtcata gatgaacgcc ttgtcagtcg ggctgttcag ctcatattcc tgggcaccgt
     2401 ccacttcccc gcgggagacc ttgccggtca ggccgccgga aacaaacacg cgcccatcgc
     2461 caagccgaac ggccgccggg aacgccgcgt gaacaaactt ctgggcgccc ttgtccgctt
     2521 cagtcacgcc gtcggcatac tgctgggaaa tgcaggtgaa cgaatcgtaa cgcgaaagaa
     2581 ccatgtcgat atcggaagcg cgatcctcga cgatattgac gttgcgggcg cgaccgaacc
     2641 atgaaggatt cgaattgccg ccggaatatc ccagcacggt gaccactcgc ccctggccct
     2701 cgcggatgcc gtcgatttcg gcgacgcctt cctcgacgtc aaccttactg cacttgcaga
     2761 tcgggacatc gacgccgttt ctgtccttga cggtgagatt gttcaccagg actctgatcc
     2821 tgtcgatctg atcaagcggt gttggcgccg gcgccccgga gcaactgttg gtcgcagccg
     2881 acacgttcat accgaggaat cccaccccgg ttccttcctc tgcgccgtca acaggcccgc
     2941 aggccgtcag cgccaggact ggaatcatcg cgaaacacag caaaattgat tttttcatga
     3001 cccaaggtct cccggtctgt gttatcccct taggaaacaa ggatagatat tgcacacaag
     3061 gttagattag tcgattgcga aaggcaagta attatcgcct tgatccgttg atttcacagg
     3121 ggccggacgg tggcttccgg ggcctcgccg ggactgatcc cggcatgtcc cgacaggctg
     3181 ggcgggcaag gcttcagtca cacgccctga cgacgcggat cgaatcgacg aagatgcctt
     3241 cgccggtgtt ttccagcgca tccaccgaat cgaagctgaa gcgcagccga atgttcttgc
     3301 ccttgaaggt gttcaggttg attccggtga cctcgaacca gttgcgataa acggccgatg
     3361 gtaccgcggc ctttgagaac accgtggtcg gcgtcagccc gtccatcacg tcaacaacga
     3421 acttgtcctt ggccgaatcg gtctgcacgt caacgaatac cgcgaatgaa agctcgtaat
     3481 cacccgtggc gggcacgcgg aattccggcg acgtggcctt gcccctggcg atgccaccgt
     3541 tgtcgaagtt ggcgttgtcg ccgttgccga agtacatcgc gcgggtgccg ccgtggaagc
     3601 gacggtcgct cagatgccag cgaacgtcgg ccccacccga cccgggaacc gagtcgttga
     3661 ccacggcgaa accgtcaaga tcgccaaaga tgtcgaatgt gtagtccgca cggacatcct
     3721 cgaagcagca tcccggcttc tcgatgaatt catgcatgca ctggtttgcc acgcagccat
     3781 cggacgtgca gtcgtcattg tcgttgcagt cctgctggac ggcgcagcaa cctgtggccc
     3841 cgacaagctg gacgtcgcag acgttatcga cgcaaccgcc aacccggcag acgccgtcaa
     3901 cgccacagtc cgtattggcc atgcattccc ttgccttgca cttgtcgtcg ataaccttca
     3961 cgttatcgat atagacgccc tcgaaattgt tgaattcctc gttgccggtc tcgaacctga
     4021 acctgacacg gacgaccttt cccgcccagg gcgacagatc gacaccgacc ggaatgaaca
     4081 cgccgccggt ggttccgtga atgttatgcg acgaccacac atcggtgacc gcctcggtcg
     4141 gctggcccat cctttccacg acctgaaccg tgaacaaatc ccactgggcc gggttgtagt
     4201 aattggccgg ggtcacgtta tcccattcgg tcgaaagcat caggtcgaat gacaggaatc
     4261 cgttatatcc ggcgcgaagc tcaagctcct gggacgatgc ctcgccattg ttcatgaacg
     4321 cgccgctgtc ataggtgcgc gtgaccgtgt ttccataata aagggcccag gcgccggtcg
     4381 cggacttgaa actgctgggc tgccatttga cggtctcatt gtccatgtaa gcccagagtt
     4441 cgaaaccacc ggtcgtcccg tcctcgaacc cggtcgaaag cagttcgaac tgcctgcagc
     4501 actgatcctc gggaagctcg gtatagacgc atgcgcctgc cgggcacgaa acggacacgc
     4561 agggatcgct gctggggcat ttggtcgcaa cgttctgcgg agtacagacc ggctcgacac
     4621 acgccggcat gatcggatgc gtgcacgcgc ccttctgttc acgattgccg aagacctggc
     4681 acttatcgtc ggtgcaggcc tcctcgtcgg ccgtgcactc ggttcccgaa tcgcactgcg
     4741 cggaacagat cgagaacacc ttcacttcat cgacataaac gccctcgaac aggttgtttg
     4801 aaccttcaag cgaatcgaac gaaagacgca gagccgcctc cttcccgacg gcgctggaca
     4861 cgttgccgct gatatagacg aagacgccgt ttgaattctt gtcaacgatc gtcgaactga
     4921 caaccagttc ggtgacatcg gtttcgccgg ccgtcgtgac gatggacatc cggaactgat
     4981 ccggctggac ttccttggga atgccgccaa tcatcggttc ggactcgacc cagatatacg
     5041 ccgacgcgat gaaggtcgct tccgcggaac tgatgggcgg aaggaagaac ttcgtggaag
     5101 tcatcgaagc gcggacggcc tcggaatcct tctgctcggg atcaacgggc tcgcagttgg
     5161 cgtcaagggc gccggagtag taagtgaggc aggtcggatt cccgaaatag gccgactttg
     5221 agccaaaggc ccggcggttg gcggcaatct gccagccgac aaaatcgttc ggcctgggat
     5281 cgttgatggt ccatccggac agatcgccct cgaagtcctc gtggaagtaa accgtttcca
     5341 tgcagcaggc tgaatcccag ccggcaacgc agaaattttc ttccccgcag acctttttct
     5401 ggcactgccc aagctcgacc ggcgcatcga cgcagtccga attcttctgg cacggcacgg
     5461 gaccggtatc ctcggaaacc tctttcgtgt ccccgggatt aagatccggc ccgacttccg
     5521 gcacatcggc aacaacgtcc tcctggccct gatccccgcc gttatcgggg taattgacat
     5581 cctcgccgcc accgaaatcg ggcgtggtat tgccgccgcc gcacccgatg accacaacag
     5641 caataagcgc cgcgaaaatc caggaaagcc tgtgcatcgc cattcctccg gaaaacagac
     5701 aaaaagaaag acgacaaatg ttatcacatc tgcgattgct agatcataat ttgaatgccg
     5761 gcgggtcaac agcccgggtg cggacggagc cagtcccctt gcagcccagc acttttcttg
     5821 acacccaccg gaaagcaaaa gattattgga caggtcgtaa ctggacatta agtatgggct
     5881 tgtttggcca gcatggcgga cttgttggac tcgacatcgg atcgagctca atcaaacttg
     5941 tacgactgaa gcaatcggcc ggttcctggg cgctgcaggc tttcagcctt gggcacctgc
     6001 ccccggacgc cattatcgac ggaagaataa tgaatttttc cgtggtgatc gagcgcctgc
     6061 gcgagctgat gcgcgaggcc gggatcaagg gcggatcgtg tgcaatcgcg atttccggcg
     6121 cgtccctgat cgtcaagcgc ctttcgcttc ccgagatgac ccgggaggaa ctggccgagt
     6181 caatcacgtg ggaagccgag caatacatcc cgttcgacat caaggacgtg aacgtcgatg
     6241 cccagatcat caattcgcgc gccggccagg gccagatgga cgtgatgatg gtcgcggcaa
     6301 agaaggacgt cgtgaacgaa tacgtttcgg tcgcgaccga agccggcctt gtaaccgaag
     6361 tcgtcgatgt cgccatcttc gcggctttga acatgttcga gacgaactac acggttccgg
     6421 cggaccagac gatcgccctg ctgaacatcg gcgccaatac tattaatgtt
//
LOCUS       DAOPPA010000139         6462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13114_length_6462_cov_4.068675, whole genome shotgun sequence.
ACCESSION   DAOPPA010000139 DAOPPA010000000
VERSION     DAOPPA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13114_length_6462_cov_4.068675"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PKH54_05680"
     CDS             complement(<1..1353)
                     /locus_tag="PKH54_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOC99414.1"
                     /translation="MNSEHLPRTALIAGLAIAFSGAAASAAPNLQVVNLTVDPPSIGQ
                     GQKATLEYMLGNNGNMDTSAFKVGFYFSEDSVVNASDTLLATVDIQTIAATTNLGEMT
                     VEVTIPTGASLGTRYIGAIADPTNAVVENNESDNSNSVLVSIVAAPDLTVTALTVSPT
                     TAVPGGDVEISYTVTNQGASPTGTFTTRLYYSDDAVITAGDTALSHERETTLAAGSTT
                     GTVVAVVKLPATASPGSRYVGAITDSKYAVAEGNENNNTRAANISIGAPTCFGKAASD
                     AAVCGGNGTCVAADTCSCAPGYSGSECETFNDCSGQPDWTSCGQGVCFDEVCEPAGEN
                     NRCQDAIGLEIGGQALGDLEGFRPFETVPQGCIGKEISGPDAFFSFQPVSGHTYVVTA
                     TPHASMDIALVAWRDCTLSDLYCLTGSDAAGNGQPEEITIDASDETVILHVVGVNAAT
                     T"
     gene            1532..2563
                     /locus_tag="PKH54_05685"
     CDS             1532..2563
                     /locus_tag="PKH54_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99415.1"
                     /translation="MKSVIFPIHPFVLAFAISFASGCGDTGPGLPDAGHDVVAPEDVN
                     APDDVINPGDVPLEPDGVEQPAPEGRVLVAVTGWGGDYGTEAWVNADFYDAPMRMDAF
                     SAYSPGMYQVAARKGDCVLYTASTAACEPACDWDRYCVAGNRCLVHPSRLSAGKLTVK
                     AGSATISADPSGDEWMPNYYYFEGQIPTSQLDATKTLSVTAEGADGGYAAFDVSQPGF
                     DGIEFVNDLDNNAFELKDDQDFLLEWTAPSGDLLNLFVEVVINVGWHGSPPESILYCR
                     KGAVTGSLTIAKEVIAQLPVIGGIALMQHGSHVGLVRNVVTDVAGKRFDFTVTRRHGI
                     NPAHNAPEF"
     gene            2590..3780
                     /locus_tag="PKH54_05690"
     CDS             2590..3780
                     /locus_tag="PKH54_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99416.1"
                     /translation="MMRLVVLLAMSLGFLVACGGSDPSVDVVADTTAPDAVDTVEPDP
                     GQPGDVGAGDGHSDLGRDIGIDSIGDGGQGDDVPDASDSVQDTEYLDQSGDPGEDVPD
                     PTDAGDSREPDQIRQPSPHGRVQVAVYKAGSSYNDRAQVDVELYDLPMRMDAFGAYSP
                     EMYQVWLAEGDCVQYFPVEYACNPPCDWDKLCVSGNRCQAAASRQSAGDMTLRFGSTS
                     FESKPTGTEDNPYWYSFDNVDAGLVDPATTVTVAADGFDGRYGAFEISVGGVGPAEFF
                     LDDEWGTLDLDDENGNEITWTVPKGDLNGMVVEVTINTGWHGAPPAAVIYCVKPVSAG
                     SLTIPASIAKQMPPMGGIGLFQHMSGIAMSRSGFADVQGRTVQLHVARLHNLNVTHNL
                     EGDL"
     gene            3780..5726
                     /locus_tag="PKH54_05695"
     CDS             3780..5726
                     /locus_tag="PKH54_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC99417.1"
                     /translation="MNVRPPMARIVTGTVACMAALAISCAVDDPVCMWQGEGRPEVLH
                     ELGCPADHEALSGQPESAALPASRSVLFMIDRTDGNKIHFFDSERWRHFMYASEMLTG
                     YTDLFAFNAEMYYQPGRRLLLGTLAHHLGPDVFAVEIVPIDKASPEMIAQMYQAVKDA
                     IRFPCDLRYHPTSTALEMESRLPPGVPVVTTSELYEGATYQGMHLGETVGRVKRMRLE
                     DLESTYVSRLDLVVLDRVPNDIPAVAGLVTGEFQTPLAHVNLLAEARGTPNMALSGAY
                     DDPRFVEMDGQWARLLVRADGFSLSPSTASEADQFWAERRPKQTQVPALDLSVTDIID
                     LDDVDASMTAAVGGKSANFGELRNITPPLPVPGGFAIPCSWYVDFMKANGLDKEMSAL
                     MAQTKFKEDGLYRMQALASLRDRMRAAPLDPGVESAILAAIDTHISGSWPIRFRSSSN
                     VEDLEGFNGAGLYESASFKPGDPEKTLAHALKKVWASMFSTAAFEEREWARIDHGRCA
                     MGVLVHRSFPDEIEAANGVAVTANPFDPPPMGQAAYYVNVQAGATSVTNPEPGVTPES
                     FLYWKPPAGQGEMTYYSSSSLTGGDKVLSFAEIQELITALRAIHRHFESIYGKIQVYG
                     MDVEFKFELPNRELVIKQARPYVF"
     gene            complement(5732..>6462)
                     /locus_tag="PKH54_05700"
     CDS             complement(5732..>6462)
                     /locus_tag="PKH54_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99418.1"
                     /translation="VRSDVDVLAGLAWLDEPGIIQTNAGAFGELLGYDLSQGLATGMT
                     PGFSLYALSRHRQDLAQKNFNAFRNIPTASGNVTEDFATKGFRPLMPAYDDTGEFGEI
                     WARFRPWEGGQDAEAIIFYLTGLWPDAAGRRVTLHPHIPNNLPGATYRGLLAAGCGID
                     MKLVEKSDATREYELKFTGGKCAGLDVTLKTATWMCGETDTCEPFVSASGCDIDATAA
                     GTGIARFTADDGMTCTFLAELIQP"
BASE COUNT         1172 a         1886 c         2092 g         1312 t
ORIGIN      
        1 ggttgtcgcg gcgttaacgc cgacaacatg taggattacg gtttcgtccg aggcatcgat
       61 tgtgatttct tcgggctggc cgttgccggc ggcgtccgat cccgtgaggc agtaaagatc
      121 cgacagcgtg cagtcccgcc aggcgacaag cgcgatgtcc atgctggcgt ggggcgttgc
      181 cgtcacgaca taggtatgtc ccgataccgg ctggaagctg aagaaggcgt ccggcccgga
      241 tatttccttg ccgatgcagc cctggggaac ggtctcgaat ggcctgaatc cttccaggtc
      301 gcccagtgcc tgcccgccga tttcaagccc gattgcgtcc tggcatcgat tgttttcacc
      361 ggctggttcg cagacctcgt caaagcacac gccctggccg cagctcgtcc agtccggttg
      421 accggaacaa tcgttgaagg tttcgcattc ggaccccgaa tatccaggcg cgcaggagca
      481 tgtgtccgcg gcaacgcacg ttccgttgcc gccgcacaca gcggcgtcgg atgcggcctt
      541 tccaaagcac gtcggcgcgc cgatgctgat gttggcagcc ctggtgttat tgttttcgtt
      601 tccttctgcc accgcgtatt tggagtcggt gatggccccg acatatctgc ttcccggcga
      661 cgcggttgct ggaagtttca ccacagcgac caccgtgccc gtggtcgagc cagcggccag
      721 ggttgtttct cgctcgtgcg aaagcgcggt gtccccggcg gtgattacgg catcgtcgga
      781 atagtacagg cgggttgtaa acgtcccggt cggcgaagcc ccctggttcg tcacagtgta
      841 gctgatttca acgtcgcctc cgggaactgc ggttgtcggc gaaacggtca gcgccgtgac
      901 agtcagatcc ggcgcggcta ctatggaaac aaggaccgaa ttgctattgt cggattcgtt
      961 gttttcgacg accgcgtttg tcgggtcggc gattgcgccg atataccttg ttccaagtga
     1021 tgcaccggtc gggatggtca cctcaaccgt catttccccg aggttcgtcg tggcagcgat
     1081 cgtctggata tcgaccgtag ccagaagcgt gtccgaggcg ttgaccacgg aatcctcgct
     1141 gaaatagaag ccaaccttga aggcggatgt gtccatgttg ccattgtttc ccagcatgta
     1201 ctcgagcgtt gccttctggc cctggccgat tgacggagga tccacggtca ggttgactac
     1261 ctgcaggttc ggggccgccg acgccgcagc cccggaaaaa gcgatcgcca ggcctgcgat
     1321 aagggcggtg cgtggcaagt gctctgaatt catgtccagt tccccttctg taatggattt
     1381 gccagtcaag cgtttccaat cataacagag atctgccatc ggcaagaagt gccgcgccag
     1441 ccggggccgc cgttcgggcg cggacgccgc gttcatctgg cgcctgccgg accaaaaatg
     1501 taaactggcg tcatctcgtt tgggtgcttg catgaagtcc gtaattttcc cgatacatcc
     1561 ttttgtgctg gccttcgcaa tttcctttgc ctcgggctgt ggcgacacgg gtccgggctt
     1621 gcctgatgcc ggccatgacg ttgtcgcgcc ggaagatgtg aacgctccgg acgatgtcat
     1681 caatccgggc gatgtgcccc tggagccgga cggggtcgaa cagccggccc ccgaaggccg
     1741 cgtccttgtc gcggtgaccg gatggggtgg cgactacggt accgaggcat gggtcaacgc
     1801 cgatttctat gacgcgccga tgcggatgga cgcgttttcc gcctattcgc cgggcatgta
     1861 ccaggtggcg gccaggaagg gcgactgcgt gctttacacc gcatcgaccg cggcctgcga
     1921 gccggcatgc gactgggaca ggtactgcgt ggccggcaac cgttgcctgg ttcatccatc
     1981 caggctgtcg gccggcaagc tgacggtcaa ggccgggtcc gcgacaattt cagccgatcc
     2041 gtccggcgac gagtggatgc ccaactatta ttacttcgag ggacagattc cgacgtcgca
     2101 gttggacgca acgaagacgc tgtccgttac ggccgagggc gccgatggcg ggtatgccgc
     2161 gttcgacgtc agccagccgg gcttcgacgg gatcgaattc gtaaatgacc tggacaacaa
     2221 cgcgttcgag ttgaaggacg accaggactt cctgctggag tggacggccc cgtccggtga
     2281 tctgttgaac cttttcgtcg aggtcgtgat taacgtcggc tggcatggaa gtccgcctga
     2341 gtcgatcctg tactgccgga agggggccgt gaccggatcc ctgacgattg cgaaggaagt
     2401 catcgcccag cttcccgtca tcggcggtat cgccctgatg cagcacggca gtcacgtcgg
     2461 cctggtgcgc aacgtggtga cggacgtcgc cgggaaacgg tttgatttca cggtgaccag
     2521 gcgccacgga atcaatccgg cacacaacgc cccggagttc tgaccagaca aaacacgacc
     2581 ggaggttgaa tgatgaggct ggttgtattg cttgcgatgt cgctgggctt ccttgtcgcg
     2641 tgtggcggtt ccgatccgtc cgtcgatgtt gtcgccgaca cgaccgcgcc tgatgccgtt
     2701 gacacggtgg agccggatcc aggtcagcca ggggacgttg gggccggtga tggtcacagc
     2761 gacctgggcc gcgacatcgg tatcgattcg attggtgatg gcggccaggg cgacgatgtc
     2821 ccggatgcgt cggattcggt tcaagacacg gaataccttg atcagtcggg agatcccggg
     2881 gaagacgtgc cggatccgac cgatgcgggt gattcgagag agcctgatca gatccggcag
     2941 ccttcgccac atggacgcgt ccaggtcgcg gtgtacaagg ccggcagcag ctacaatgac
     3001 agggcgcagg ttgacgttga actgtacgac ctgccgatgc ggatggacgc gtttggtgcc
     3061 tacagtccag agatgtatca ggtctggctg gctgagggcg actgcgtcca gtacttcccc
     3121 gttgaatacg cgtgcaaccc gccgtgcgac tgggacaagc tgtgcgtttc cggcaaccgg
     3181 tgccaggccg ccgcttcaag gcagtcggcc ggggatatga cacttcggtt cggatcgacc
     3241 agtttcgagt ccaagccgac tggaacggaa gacaatccgt actggtattc cttcgacaat
     3301 gtcgatgccg gcctggtcga tccggcgacc actgtcaccg ttgcggccga cgggttcgac
     3361 ggccgctatg gggcgttcga aatctcggtc ggcggggtcg ggccggcgga gttcttcctt
     3421 gacgacgaat ggggcacgct ggatctcgac gatgaaaacg gcaacgaaat aacgtggacc
     3481 gtgccgaagg gcgatttgaa cggcatggtg gttgaagtca cgatcaacac cggctggcac
     3541 ggtgcgccgc cggcggccgt catctactgc gtgaagcccg tctcggccgg cagcctgaca
     3601 attccggcga gcatcgcgaa gcagatgccc cccatgggcg gaattggcct gttccagcac
     3661 atgagcggca tcgcgatgtc caggtcgggg ttcgcggacg ttcagggccg gacggttcaa
     3721 ctgcatgtgg cgcggctgca caaccttaac gtcacgcaca atctggaggg ggacctgtga
     3781 tgaatgtccg cccgcccatg gcccggatcg tcaccgggac ggtcgcctgc atggccgccc
     3841 ttgcgatatc gtgcgccgtg gatgatccgg tgtgcatgtg gcagggcgaa gggcggccgg
     3901 aagtgctgca cgagctgggg tgcccggccg accacgaggc cctttccggc cagcccgaat
     3961 cggcggcctt gccggcaagt cgttcggttc tgttcatgat tgacaggacg gacggcaaca
     4021 agatccattt cttcgattcc gagcgatggc gtcacttcat gtacgccagc gagatgttga
     4081 ccggatacac cgacctgttc gcgttcaatg ccgagatgta ctaccagccc gggcgcaggc
     4141 tgctgctggg aaccctggcg caccaccttg gaccggacgt cttcgcggtt gagattgtgc
     4201 cgatcgacaa ggccagcccg gagatgatcg cccagatgta ccaggcggtt aaggacgcga
     4261 tccggttccc gtgcgacctg cgttatcacc cgacatcaac cgcgctggaa atggagtcca
     4321 ggcttccgcc gggcgtgcct gtcgtgacaa catctgaact ttacgaaggc gcaacctacc
     4381 aggggatgca tctgggcgag acggttggcc gcgtcaagcg gatgcgcctg gaagaccttg
     4441 aatcaacata cgtttccaga cttgacctgg ttgtcctgga tcgcgtgccg aacgacattc
     4501 ccgcggtggc gggacttgta accggcgagt tccagacgcc gcttgcacac gtaaatctgc
     4561 tggccgaggc gcgtggcacc ccgaacatgg cgttgtccgg cgcgtacgac gatccccgtt
     4621 ttgtcgagat ggatggtcaa tgggcgcggc ttcttgtaag ggcggatgga ttttccctgt
     4681 cgccatcgac agcgtcggaa gcggatcagt tctgggccga aaggcggccg aagcagaccc
     4741 aggttccggc tctggatctt tccgtgacgg acattatcga cctggatgac gtggatgcgt
     4801 cgatgacggc ggcggtcggg ggaaagtcag cgaatttcgg ggaattgcga aatataaccc
     4861 caccgttgcc cgtcccgggt ggctttgcaa tcccgtgttc ctggtacgtc gatttcatga
     4921 aggcgaacgg gctcgacaag gagatgtcgg ccctgatggc gcagacgaag ttcaaggaag
     4981 acgggcttta caggatgcag gcccttgcgt cgctccgcga caggatgcgc gccgcgcccc
     5041 tggatccggg cgtcgagtcc gccatattgg cggcaatcga cacgcatatt tccggttcgt
     5101 ggccgatcag gttccgttca agcagcaatg tcgaggatct ggaaggtttc aatggggccg
     5161 ggctctatga atcggcttcc ttcaagccgg gggatccgga aaagacgctt gcgcatgcat
     5221 tgaagaaggt ctgggcaagc atgttcagca cggccgcgtt cgaggaacgc gagtgggcca
     5281 ggatcgatca tggcaggtgc gccatggggg tgctggtgca ccgctcgttt cccgacgaaa
     5341 tcgaggccgc gaacggcgtt gccgtgaccg cgaacccgtt cgatccgccg ccgatgggcc
     5401 aggccgccta ttacgtcaac gtgcaggctg gcgcgaccag cgtaactaat ccggaacctg
     5461 gagtgacgcc ggaatctttt ctttactgga agccgccggc cgggcagggc gagatgacat
     5521 actattcaag ttccagcctt actggcggcg acaaggtgct ttcgtttgcc gagatccagg
     5581 aattgattac ggccttgcgg gcgatccatc ggcatttcga atccatctat ggaaaaattc
     5641 aggtctacgg catggacgtg gagttcaagt tcgagcttcc caatcgcgaa ctggtcatca
     5701 agcaggcgcg tccgtatgta ttctgatggc atcagggctg gatcagttcg gccaggaagg
     5761 tgcaggtcat gccgtcgtcc gcggtgaagc gggcgatgcc cgtccctgct gcggtcgcgt
     5821 cgatgtcgca gcccgacgcg ctgacgaacg gttcgcacgt gtccgtttcg ccgcacatcc
     5881 aagtggcggt cttcagcgtc acgtccaggc ccgcgcactt gccgccggtg aacttcagtt
     5941 catattcgcg ggtcgcgtcg gatttctcga caagcttcat gtcgatgccg catccggccg
     6001 cgagaagtcc gcgataggtc gcgccgggaa ggttgttcgg gatatgcggg tgcagcgtga
     6061 cgcgccttcc cgccgcgtcc ggccacagcc cggtcaggta gaagatgatc gcctcggcgt
     6121 cctgtccgcc ttcccacggg cggaagcgtg cccagatttc cccgaactcg ccggtgtcgt
     6181 cgtatgccgg catcagaggc ctgaatccct tcgtcgcgaa gtcttccgtg acatttccgg
     6241 atgcggtcgg gatattccgg aatgcgttga agttcttctg cgccaggtcc tgccggtgcc
     6301 gggaaagcgc gtacagcgag aagcccggcg tcattccggt cgcaaggcct tgcgaaaggt
     6361 cgtatccaag cagttcgcca aaggcgcccg cgttcgtctg gatgatgccg ggttcgtcca
     6421 gccaggccag tccggcaagg acatccacgt cgctgcgcac cc
//
LOCUS       DAOPPA010000140         6452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13146_length_6452_cov_3.767704, whole genome shotgun sequence.
ACCESSION   DAOPPA010000140 DAOPPA010000000
VERSION     DAOPPA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13146_length_6452_cov_3.767704"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKH54_05705"
     CDS             <1..324
                     /locus_tag="PKH54_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99419.1"
                     /translation="ANLNQAVEKQKIRNIVSNVEVITAEGKAAVDEAKLREIVVKVAS
                     LIDTTEGTVHNIDTTVLRAKDDIFSSLTYLDEALENLSEFARMIRENPGLILGGSQEK
                     ERKLP"
     gene            321..1067
                     /locus_tag="PKH54_05710"
     CDS             321..1067
                     /locus_tag="PKH54_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOC99420.1"
                     /translation="MMKNKAFTITLGIIVLLLMLPLAGGCGTGPQRKYYTLAYAIDDN
                     PANFERAPLYPVSIRVKRFTIGEPYNRPQLVYRQSPFEFMYYGFRYWAAKPQKLLRDI
                     IKEHIQSLNIVNSIYLEYTNKVPDYELGGNIESIEEFDSGGDWYAHLALRLELTRFSD
                     KTVIWRYSFDRKKKVEQKNPVFVVKAMSELLKEEMAVIAAQIDAALAGERGSKPTLTC
                     PATPVDEIPVLETFPSQPDKLLKEERPDAK"
     gene            1436..2746
                     /locus_tag="PKH54_05715"
                     /pseudo
     CDS             1436..2746
                     /locus_tag="PKH54_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2846..3856
                     /gene="metX"
                     /locus_tag="PKH54_05720"
     CDS             2846..3856
                     /gene="metX"
                     /locus_tag="PKH54_05720"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOC99421.1"
                     /translation="MPGGLTLESGVRLPELRLAYRTWGRLNQAGDNAVVLCHALTGSA
                     DADDWWKGLIGSGCATDPADDFIVCANLPGSCYGSTGPLARNPATGRPWGPDFPALTV
                     RDMVAAQAALCRQLGARSIRTVIGGSLGGMMALEFAAMYPEMVRSAVSIAACARHSAW
                     CTGLSEAQRATIMADPDWCGGRYDRTCPPAAGLAAARMTAMCTYRSHESFQARFGRTI
                     QDDGRFAVESYLHHQGDKLVGRFDANTYMILTRAMDTHDLGRGRGGLQAALAAIRIPV
                     LVVSYDSDVLYPTADQAEIAAGIPGARLETLHSPHGHDAFLIDIAQLARLIANFRAEV
                     KS"
     gene            3853..6354
                     /gene="thrA"
                     /locus_tag="PKH54_05725"
     CDS             3853..6354
                     /gene="thrA"
                     /locus_tag="PKH54_05725"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOC99422.1"
                     /translation="MSDWKVLKFGGSSMGAPDRIAQVIEIITATSAGCPTAVVVSAMQ
                     HTTDRLIEASELAAGGDLEAAGRIVDTVMDLTTSNGLFVLQNLPGGKNGGSGIIPLIR
                     AMTDELRQLLYAMSLLHEQTPQTLDLILSFGERLSASVLTELLKAAGLPAVFVDSRQW
                     TVTDAGFGAAVVDQDATRASLRELAADWDGRVPVTTGFLGRTPDGRTTTLGRNGSDYT
                     ASLLARGLKASEVVVWTDVSGVMTADPGIVADAYPLARMSYMEALEMVDFGATLFHPR
                     TMIPLIEDNIPMRIRNTMRPDYPGTLIDATGSQDESAATSVTSMENLALLSIQVRRIA
                     MRAQASVRVLGALEQAGVTVWMSTLSAHGHSAAAVVRRADAARASDAISREFILELQR
                     GEIEPVSLREPVTLLTLVAEAMGRTTNVAGRFFNSLGMMGIPIRASAQGASERSISCV
                     IDAADTQVAVRTVHSAFNFSHQDVSLFILGKGSVGGNLLTQIRDERDRLERECDVLLR
                     VVGIRDSRALIFDDRGIDLDRWGELLQAAGPGDVLDRLDDLRRLPVPILVDTTGLDGM
                     EEIYRQALSRGIHVVAANKKPLTIPGAERAELFATARRNHRVYLYSTTVGASLPVIET
                     LKNLVRTGDHVRRIEGSFSGTIGYITNELMAGVPLSQATRIARELGYTEPNPQDDLGG
                     MDVARKALILTREMGLELEMSDLVVEPLLPPELMTPMSPDAFFESLKAYDRTMAERIA
                     AIKESGKIMRYLAIVDTHATGTPQDPVVKVGPVLIERDHPAALLRGTEAFVAFTTDRF
                     LDYPIIVQGSGAGGVVTAAGVLSDILKVSMTLRGR"
BASE COUNT         1238 a         2059 c         1982 g         1173 t
ORIGIN      
        1 gcaaacctga accaggcagt cgaaaagcag aagatccgga atatcgtcag taacgtcgag
       61 gtcatcacgg cggaaggcaa ggcggccgtc gatgaggcga agctgcgcga aatcgtcgtg
      121 aaggtggcaa gcctgatcga cacgaccgaa ggaaccgtgc acaacatcga cacgaccgtc
      181 cttcgtgcaa aggacgacat cttctccagc ctgacgtatc tggacgaagc acttgaaaac
      241 ctttcggaat tcgccaggat gatacgcgag aacccgggtt tgattcttgg tggatcccag
      301 gaaaaggagc gcaagctgcc atgatgaaga acaaagcttt cacgattacg ctgggcatta
      361 ttgtgcttct gctgatgctt ccgcttgcgg gcggatgcgg aaccggtccc cagcgcaaat
      421 actacacgct cgcatacgca atcgatgaca acccggccaa tttcgagagg gccccgcttt
      481 atccggtaag catcagggtc aagcggttca cgatcggtga accttacaac aggccgcagc
      541 ttgtttaccg gcaatcgccc tttgaattca tgtactacgg gttccgttac tgggccgcca
      601 agccgcagaa gctgctgcgc gacatcatca aggaacacat ccagtcgctg aacatcgtca
      661 attccattta cctggaatac accaacaagg tgcccgacta cgaactgggc ggaaacatcg
      721 aatccatcga ggaattcgat tcaggtggtg actggtacgc acacctggcg ctgcggctgg
      781 aactgacccg tttttccgac aagaccgtga tctggcgcta ttcattcgac cgcaagaaga
      841 aggtcgagca gaagaatccg gtgtttgtcg ttaaggcaat gtccgagttg ctgaaggaag
      901 agatggctgt tatcgcggcg cagatcgacg cggcccttgc cggggaacgc ggttcgaaac
      961 cgaccctgac ctgccctgcc acgccagtgg atgagatacc cgtcctggag actttcccga
     1021 gtcagccgga caagctgctg aaggaagagc ggccggacgc gaagtaggct gccaccgagt
     1081 attcgtttac ccccgtggac gcccaaaatt gtgacaacaa ctgtcatttt tcagaatttt
     1141 gttgaaatat cagagcgaat cgtcataatt ggggcaccac accggctttc tgacggtggc
     1201 acaaagggac acgatgaagc caggatccac aaagtctgat gtcaaatcgg gcgaaagctg
     1261 gcccgccgac atcacccgcc cagggacgca tcccgtatgt cgaatgcgcg gcggtgcggc
     1321 cgccgtccgt tgaccggtcg caccagttcc cctgaaacac atgaaggatt gctgcctgcc
     1381 gcatggcagg acgaatatgt cgtgcgaagc gatgcacggc ttacgggagc gtgaaatgca
     1441 acggatactg aacaagctgg tcgtggatga ttgctgcgac gacgccgggc ctggcatcga
     1501 gactatcctg gttcatgccg gccagacgcc cgatcccgca accggggcgc gctccgtccc
     1561 gctttaccag acggccagtt atgtgtttga aagcgccgat catgccgctg acctgttcgc
     1621 gttgaagcgt gatggaaaca tttacacccg gctcggcaac ccgacatccg acgtgtttga
     1681 acagagggtt gcggccatgg agggcggctc gggagccctg gcgacctcca gcgggatggc
     1741 ggccatcttc cttgccgtgc acacggtggc gcgcgccggc gatcacatgg ttgccgcggc
     1801 atccctttat ggaggtacgg acaccctttt ccgttacacc ttcgaacggc tgggcatcga
     1861 cgtcaccttc atcgacgacc cgacacctga aagcgtcctt ggcgcaatgc ggccggaaac
     1921 gcgcctggtt ttcatcgaaa ccatcagcaa tcctggcggc gacgtcccgg atatcgccgg
     1981 aatcgcggcg gcggcccatt cggccggcgt tccgctgttc gttgacaaca cgttcgctcc
     2041 ggttctttgc cgcccgctgc ggcacggggc tgacgttgtg attcactcgg ccaccaaatg
     2101 gatctgcggc cacggaacct cgattggcgg cgtcatcgtg gatggcggcc gattcgactg
     2161 gacttgcggc cgattcccgg acttcaccac gcccgacgaa agctaccacg gggtcgttta
     2221 tgcccgcgac ttcggcaata tggggttcat tcgcaaggct cgccttcagg gccttcgcaa
     2281 catcggcatg tgcgcgtcgc ctttcaacag tttcatgttc cttcagggac ttgaaacgct
     2341 tcacctgcgc atccgcgaac agtgtgccgg cgcgctgaag ctggcccgct ggctggcccg
     2401 ccgccccgaa gtgctgtggg tcaactaccc cgggctgcca gggcatccgt cgcacgaaaa
     2461 cgcccggcgc ttcctcgccg gcggcttcgg atctgtcctg ggtttcggta tccgcggagg
     2521 ggcggcagcc ggcgcaaggt tcatcgattc agtcaagctg gcgtcgcacc tggcaaacat
     2581 cggcgacgca aagaccctgg ttctgcatcc ggcttcaacc acccaccagc agatgccccc
     2641 cgaaggcctt gccgcctgcg gcataacacc cgaattcatc agggtttccg ttggccttga
     2701 aaacattgcc gacattatcg ccgacttccg ccaggccttt gccgcggcat gcgggactga
     2761 cggggcctgc ggcccgggcg gggcctttga agtacagact ggcgacactg cggcaccact
     2821 ttctgacctg acgaaattcg cggacatccc cggcgggctt accctggaga gcggggtgcg
     2881 cctgccggaa ctacgccttg cgtaccgcac ctggggccga ctgaaccagg ccggcgacaa
     2941 cgcggtcgtc ctttgtcacg cgctgacggg ctcggcggac gcggatgact ggtggaaagg
     3001 gctaatcggt tccggatgcg cgaccgaccc cgcggacgac ttcatcgtgt gcgcgaacct
     3061 tccgggaagc tgctatggct cgaccgggcc tcttgccagg aaccccgcca cgggacgtcc
     3121 atggggtccc gacttccccg cgctgaccgt ccgcgacatg gtcgcggcac aggcggccct
     3181 gtgcaggcag cttggcgcac gatcgattcg cacggttatt ggcggctccc ttggcggcat
     3241 gatggccctg gagttcgcgg cgatgtaccc ggagatggtg cggtcggccg tgtcgatcgc
     3301 ggcctgcgca cgccattcgg catggtgcac ggggctttcc gaagcccagc gcgccacgat
     3361 aatggccgac cccgactggt gcggcggccg atatgatcga acctgcccgc cggccgcagg
     3421 cctggcggcc gcgcgcatga ccgcgatgtg tacctatcga agccacgaaa gcttccaggc
     3481 gaggttcggc cggacgatcc aggacgatgg ccgattcgcg gtcgaatcat acctgcacca
     3541 ccagggtgac aagctggtcg gccggttcga cgcaaacaca tacatgatcc tgacaagggc
     3601 gatggacacg cacgacctgg gccgcgggcg cggcggactg caggcggcgc ttgcggctat
     3661 tcgcatcccg gttctggtcg tttcatacga cagcgacgtg ctgtacccga ctgccgacca
     3721 ggcggagatc gccgcaggca ttcccggggc gcgtctggaa acgcttcatt cgccccacgg
     3781 acacgatgca ttcctgattg atatcgcgca actggcacgt cttatcgcca actttcgggc
     3841 agaggtgaaa tcatgagcga ctggaaagtc ctgaaattcg gcggatcgtc catgggcgct
     3901 ccggaccgca tcgcccaggt cattgaaatc atcaccgcga catcggccgg gtgcccgacc
     3961 gccgtcgtcg tttccgcgat gcaacatacg acggatcgcc tgatcgaggc cagcgagctg
     4021 gccgctggtg gcgatcttga ggccgcgggc cgcatcgtcg atacggtcat ggatctgacg
     4081 acctccaacg gactgttcgt cctgcaaaac ctgcccggcg gcaaaaacgg cggatccggg
     4141 atcatcccgc tgatccgggc catgacggac gaactgcgcc agcttctgta cgccatgagc
     4201 ctgctgcacg aacagacgcc gcaaacgttg gatttgatcc tgtccttcgg cgaacgcctg
     4261 tcggcctctg tgctgaccga actgctgaag gcggccggcc ttccggcggt attcgtggac
     4321 tcacgtcaat ggaccgtgac agacgccggt tttggcgccg cggtcgtgga tcaggacgcc
     4381 accagggcca gtctgcgcga gctggcggcg gactgggacg gccgcgtgcc cgtgaccacc
     4441 gggttcctgg ggcgcacccc cgacggccgc acgaccacgc tcggacgcaa cgggtcggac
     4501 tacaccgcct cgctgcttgc gcggggactt aaggccagcg aagtcgtcgt gtggacggac
     4561 gtttccggcg tcatgacggc cgacccggga atcgtggccg acgcgtatcc cctggcgcgc
     4621 atgagctaca tggaagccct ggaaatggtt gacttcggcg cgacgctgtt tcatccgcgc
     4681 acgatgattc cgttgatcga ggacaacatc ccgatgcgca tccgcaacac gatgcgcccg
     4741 gactaccccg gcacgctgat tgacgccacc ggcagccagg acgaatcggc ggcaacgtcg
     4801 gttacctcga tggaaaacct tgccctgctg agcatccagg tccgccgcat tgcaatgcgc
     4861 gcacaggctt ccgtacgtgt ccttggtgcc ctggaacagg cgggcgtcac cgtctggatg
     4921 tccacgctgt cggcccacgg ccactcggcc gccgcggtgg tcaggcgcgc cgatgccgca
     4981 cgcgccagtg acgccatcag ccgggaattc atccttgaac tgcaacgcgg cgaaatcgag
     5041 ccggtcagcc tgcgcgagcc ggtcaccctg ctgaccctgg tcgccgaggc catgggccgc
     5101 acgaccaacg tggccgggcg cttcttcaat tcccttggca tgatgggcat tcccatccgc
     5161 gccagtgccc agggcgccag cgaacgctcg atttcatgcg tcatcgacgc tgcggacaca
     5221 caggttgctg tccggacggt tcactcggcc ttcaacttca gccaccagga cgtgtcgctg
     5281 ttcatccttg gcaagggaag cgtcggcggc aacctgctga cccagattcg cgacgaacgc
     5341 gaccggcttg aacgcgaatg tgacgtcctg ctgcgcgtcg tcggcatacg cgacagtcgc
     5401 gccctgattt ttgacgaccg cgggatcgac cttgaccggt ggggcgaact actccaggcg
     5461 gccggccccg gggacgtcct ggatcgcctt gacgacctgc gtcgcctgcc cgtgcccata
     5521 ctggtcgata cgaccggcct ggacggcatg gaagaaatct atcgccaggc gctgtcccgc
     5581 ggcatccacg tggtggccgc gaacaagaag cccctgacca tcccgggcgc cgaacgggcc
     5641 gaactgttcg cgaccgcgcg tcgcaaccac cgcgtctatc tgtactcgac caccgtcggc
     5701 gcaagcctgc cggtcatcga gaccttgaag aacctggtca ggacgggaga ccacgtcagg
     5761 cgcatcgaag gcagcttttc cggcacgatc ggctacatca cgaacgaact gatggctggc
     5821 gtccctcttt cgcaggcaac aaggatcgcc cgggaactgg gctacaccga accgaatccg
     5881 caggacgacc tgggcgggat ggacgtggcc cgcaaggcgc tgatcctgac gcgcgaaatg
     5941 ggcctggaac tggagatgtc cgacctggtt gtcgagcccc ttcttccgcc cgaactgatg
     6001 acgccgatga gtcccgatgc gttcttcgaa agcctgaagg cttacgaccg gacgatggcg
     6061 gaaaggattg cggcgatcaa ggaaagcggg aagatcatgc gttacctggc gatcgtcgat
     6121 acacacgcca ccggcacgcc gcaggacccg gtcgttaagg ttggcccggt gctgatcgaa
     6181 cgcgaccatc cggccgcgct tctgcgcgga accgaagctt tcgtcgcgtt cacaaccgac
     6241 cgcttcctgg attacccgat tatcgtgcag ggctccggcg ctggcggggt cgtcaccgcg
     6301 gccggcgtcc tgtccgacat cctgaaggta tctatgacac tgcgcggccg gtgaatccag
     6361 tccccctgcc ctggccttgt acgggcggat tacatccgcc cgcctgcgtg aaaattcggc
     6421 cgcttctgtc acatggtggc tgcgatttcg cc
//
LOCUS       DAOPPA010000141         6443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13168_length_6443_cov_4.776769, whole genome shotgun sequence.
ACCESSION   DAOPPA010000141 DAOPPA010000000
VERSION     DAOPPA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13168_length_6443_cov_4.776769"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1807
                     /locus_tag="PKH54_05730"
     CDS             86..1807
                     /locus_tag="PKH54_05730"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOC99423.1"
                     /translation="MNKPFELREDAATGRTFYETNLTGETLLNNWILNKGDGFTPAER
                     RELGIDGMVPTATATLDLQVERAYEQFCAKTSDLEKYTFLNGILDRNATLFFKLVLDH
                     LSEMIPIIYTPTVGTACLKFSHIFRRTRGLYINPENIGRIDDILAAVPLPRVRLIVVT
                     DGERILGLGDQGADGMGIPIGKVGLYVAAGCLHPSEVLPITLDVGTNNQSKLDDPMYI
                     GWRRNRITGDEYHKFIDTFLHACKRAFPGVMYQWEDFAKHHAFDLLAKYRKTILSFND
                     DIQGTGATALAALMGAARIKGVAMKDMKYCIAGAGEAGTGIANNIEAALKAEGLSEQD
                     ARNRIYHIDRQGLLLEDDPTLEPQQHTYAKSRAHVAGWKLKKEGFIDIIDVIRNAGVN
                     VLVGTTAVPGRFDDEILEGMAASDSRPVIMPLSNPTSKVECTPEQVCRATGGSFIMAT
                     GSPFPPIETKIGMQGIAQCNNLFIFPGVGLGAIVAQASEVTDSMFAAGARALAGMVTD
                     DQAARGYVLPEMKFIREASAQVAMAVAREARDCGVGAKLTDAEIEARVLASQWTPAYV
                     PVRKVTP"
     gene            1988..5326
                     /locus_tag="PKH54_05735"
     CDS             1988..5326
                     /locus_tag="PKH54_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOC99424.1"
                     /translation="MQISSVSNLTQMTPDDRLSVMNIQRTCVHDGPGIRTTVFFQGCN
                     MRCEWCQNPESHDFTGNRDRPHGSSIDEILAVVRRDRRFYDLTGGGVTFSGGEAMMQH
                     GPSLARLVRALKADGIHVAIETAGDAPWKNFQAVLADVDLFLFDLKYVADGSEHQRLV
                     GRPFKPVMENLARLVTAGARIEFRMCVVPGRNDSDTNIRATAELLRSLGYGSITLLRY
                     FDMHESKARRLGIARSPLGITVDQARQALARAARTFIDLGLKVDVHDPDEPRRETVFS
                     DRVNTLRRDIRDAGYSLCMESAFLKTRFYRRHGFKGPVNVKRANLLRYILNNKKVIVY
                     PHELLVGNFTAKRVGAQLWLEYFSAGGIGNIWNCDKQTPVAFKCGDLDKARYYAELAP
                     FWTRNSVITKIFPNLTELGLFAARMLEKKVGFENNMSGVSHFIVNNERLLRLGTEGIR
                     AEVLAARQAKRDAALLDFPKKDRSDDFENFYEAVLTSLTALEEFAARYARHLKKMARD
                     ESDPTRAAELRQMAEVCEHVPKHPARTFHEALQSILFMLIGLCMEAFENAISFGRLDQ
                     ILYPFLRADLDAGRITMERARELVACLVLKCDESIFFNDGDTGFELGKMFETLSPVYT
                     ITMGGTDTQGNDATNDVTYMILDACKLHPLSVNMAARIHAGSPDAYLRRIAEVYVTGT
                     PMPELFNDDVYVPALRHNYPDTTIAQSRNYSIVGCVEPCASTEHFGNTDSANVNLTMP
                     LLQALTGDTTRLWRYGGLRGIERRGLDFAAKRLATGPLTARMVDHVADRLSSPTCVNP
                     PASMDELLDRFQDRLNEVTADVLADQYLIETALAKTLPVPLASATFESAVTAGRDLLQ
                     GGAAINTSGIQAVGVTDVADSLYAIDELVFKRGAFTMQQLLDALDANFQGPKHERIRE
                     MLMAIPKFGNDADPRPAQWETRVMEAWCKALAAVPKTTRGGRYVAGYYGLNVNLAYGR
                     NTPALPSGRLAGVPLANSVVPHYGMKMVDLTGSLNAVAQVDFPNNAPNGTTNTATIDA
                     ALFPGDGGIDNLAGMIRAYFDQGGMQFQPNVFSRETLLDAYKHPEKYPNLLIRIAGYC
                     AYFNDLSDDLKLEIINRTYYSDAV"
     gene            complement(5372..>6443)
                     /locus_tag="PKH54_05740"
     CDS             complement(5372..>6443)
                     /locus_tag="PKH54_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99425.1"
                     /translation="LIENDARAFFKKTPLEDLQKKNYFFDGGIRDGLGALTSALHLAN
                     TLKDRGVDIRYYKNFTGFEDSVFPDLECKQLLGTKEDFGTVDFSRKSFGNSFLIAYGD
                     PDMDEEDAYIDGSGNHIGNGCEVNLRGLVPYAAANVRIPDPVVKLNYNFLGEIFFTSY
                     YSEVLKSRRWMSVNLPPDYDSTDCYANCTAENPEDKVLYNELEYPVSYMLPGIGMPLD
                     GMGILTMLFNLTMGYGSSPRYILIVPDGQCCFRNKTTGAQVCNCFENSDVMTCVDNEC
                     KGPHETCTVTNIPKSEFNPNHEQECNSGHFFVNQATNRYGATGEALETMKFEDALIEM
                     LTAVENTFRVRKPATVKVPKDF"
BASE COUNT         1355 a         1967 c         1907 g         1214 t
ORIGIN      
        1 cattcggtgg tatcgtgcgc agacgcccgg gtttgcctga aggcgacccc gaccgcaaca
       61 tattgaaaaa gctggagact gcgtgatgaa caagccattt gaacttcgtg aggatgcggc
      121 aaccggaaga accttctacg aaacgaacct gacaggcgaa acccttctga acaactggat
      181 tctgaacaag ggcgatgggt tcacgccggc cgaaagacgc gaacttggta tcgacggcat
      241 ggtcccgacc gcgacggcga cgctggatct tcaggtggag cgcgcctacg agcagttctg
      301 cgcaaagact tccgacctgg aaaagtacac tttccttaac ggcatcctgg acaggaacgc
      361 cacgctgttt ttcaagctgg tgctggatca tctttccgaa atgatcccaa tcatttacac
      421 gccaacggtc ggcacggcct gcctgaagtt ttcacacatc ttcaggcgga cccgcgggct
      481 ttacatcaac cccgaaaaca tcggccgcat tgacgatatc cttgcggcgg ttcccctgcc
      541 ccgagtacgg ctgatcgtcg taaccgacgg cgagcgcatc ctgggcctgg gcgaccaggg
      601 cgccgacggc atgggcatcc cgattggcaa ggtcggcctg tacgtggcgg ccggctgcct
      661 gcacccgtcc gaagtacttc cgatcacgct ggatgtcggc accaacaacc agtccaagct
      721 ggacgacccg atgtacatcg gctggcgccg caacaggatt accggcgatg aataccacaa
      781 gttcatcgat accttcctgc acgcatgcaa gcgggccttc cccggcgtca tgtaccagtg
      841 ggaagacttc gccaaacatc acgcttttga cctgctggcc aaataccgca agaccatcct
      901 ttcgttcaac gacgacatcc agggaaccgg cgcgacggcg ctggcggccc tgatgggcgc
      961 ggcccggatc aagggcgtgg ccatgaagga catgaagtac tgcatcgccg gcgccggcga
     1021 ggccggaaca gggattgcca acaatatcga ggcggccctg aaggctgaag gcctgtccga
     1081 gcaggacgcc cgcaatcgaa tctatcacat cgatcgccag ggcctgctgc tggaagacga
     1141 cccgacgctg gaaccccagc agcatacgta cgcgaagtcg cgtgcccacg tggcgggctg
     1201 gaagctgaag aaggaaggct tcatcgacat catcgacgtg atccggaatg ccggcgtcaa
     1261 cgtgctggtc gggacgaccg cggtgccggg acgtttcgac gacgaaatcc ttgaaggcat
     1321 ggcggcgtcc gacagccggc cggtcatcat gccgctttca aacccgacat ccaaggtcga
     1381 atgcacgccg gagcaggtct gcagggccac cgggggcagc ttcataatgg cgaccggcag
     1441 cccgttcccg ccaatcgaga caaagattgg catgcagggc atcgcccagt gcaacaacct
     1501 gttcatcttc ccgggtgtcg gccttggggc catcgtggcc caggcgtccg aggtcacgga
     1561 ctcgatgttc gcggccgggg cgcgcgcgct ggccggcatg gtcacggatg accaggcggc
     1621 acgcggatac gtcctgccgg agatgaagtt catccgcgaa gcatccgccc aggtcgcgat
     1681 ggcggttgcc cgcgaagcgc gtgactgcgg cgttggcgcg aagctgaccg acgccgagat
     1741 cgaggcccgc gtccttgcca gccagtggac ccccgcgtac gtgcccgtca ggaaagtcac
     1801 cccgtagcgg cgaataaaaa ttcgcccgcc ggcgatgacg cgctgcgaaa cggacagata
     1861 cataggattt tgtccgcata gaattttttc gcaattttct atgcacaatc cttgcgatcg
     1921 cccaataaat gcccaacaat acgatcaggt aacgattgtt gcacataggc aacgaaaggg
     1981 attttctatg cagatttcat ccgtttcgaa tttgacgcag atgacgccgg acgaccgact
     2041 ttcggtcatg aacatccagc gcacgtgcgt tcacgacggc ccgggcatcc gcacgaccgt
     2101 ctttttccag ggctgcaaca tgcggtgcga gtggtgccag aacccggaaa gccacgactt
     2161 caccggcaac cgcgaccgcc cgcacggttc ctcgattgac gaaatcctgg ccgtggtcag
     2221 gcgcgatcgg cgcttttacg acctgactgg cggcggggtt accttcagcg gtggcgaggc
     2281 catgatgcag catggcccca gtctggcccg cctggtcagg gcgttgaagg cagacggcat
     2341 ccatgtggcc atcgaaaccg cgggcgatgc cccgtggaag aacttccagg cggttctggc
     2401 ggacgttgac ctgttcctgt tcgatctcaa gtacgtggcc gatggcagtg aacaccagcg
     2461 cctggtcggg cgcccgttca agccggtaat ggaaaacctg gccaggctgg ttacggccgg
     2521 ggcacgtatc gaattcagga tgtgcgtggt tccgggacgc aacgactcgg acacgaacat
     2581 ccgggccact gctgaactgc ttcgttcgct tggttacggc tcaatcaccc ttctgcgcta
     2641 ttttgacatg cacgaatcca aggccaggcg ccttggcatc gcccgcagcc cactcggcat
     2701 cacggttgat caggcgcgtc aggcgctggc acgcgccgcc cgcaccttta ttgacctggg
     2761 attgaaggtc gatgtgcacg acccggacga accgcgccgc gaaacggtct tttccgaccg
     2821 agtcaacacc ttgcgtcgcg acatccgcga cgccggctac agcctgtgca tggaatcggc
     2881 gttcctgaag acccggttct atcgacgcca tggattcaag ggcccggtaa acgtcaagcg
     2941 ggccaacctg ctgcggtaca tcctgaacaa caagaaagtc atcgtttatc cgcacgagct
     3001 gctggtcggc aacttcaccg cgaagcgagt cggggcacaa ttgtggctcg aatacttcag
     3061 cgcgggcggc atcggcaaca tctggaactg cgacaaacag actccggtcg cattcaagtg
     3121 cggcgacctg gacaaggcgc gctactacgc cgagttggcg ccgttctgga cgcggaacag
     3181 cgtgatcacg aagatcttcc cgaacttgac cgaactcggc ctgttcgccg cgcgcatgct
     3241 ggaaaagaag gtcggtttcg aaaacaacat gtcgggcgtg tcccacttca tcgtgaataa
     3301 cgaacggctg ctgcgcctgg gcactgaagg aatcagggcc gaggttctgg cggcaaggca
     3361 ggccaagcgc gatgccgccc tgctggactt cccgaagaag gatcgttcgg acgactttga
     3421 gaatttctat gaagcggtcc tgacatcgct gaccgcactc gaggaattcg cggccaggta
     3481 cgcgcggcac cttaagaaga tggcccggga cgaaagcgac ccgaccaggg ccgccgaact
     3541 caggcagatg gccgaagtgt gcgaacacgt cccgaaacac cctgcccgca ccttccacga
     3601 ggctctccag tccatcctgt tcatgctgat cggcctttgc atggaggcgt tcgaaaacgc
     3661 catcagcttc ggtcgccttg accagattct ttacccgttc ctgcgggccg atctggacgc
     3721 ggggcgcatc accatggaac gggcccgcga actggtcgcg tgcctggtac tgaagtgcga
     3781 cgaatcaatc ttcttcaacg acggcgacac cggatttgaa ctgggcaaga tgttcgaaac
     3841 cctgtccccg gtgtacacca tcaccatggg gggaaccgac acgcagggca acgacgccac
     3901 caacgacgtc acatacatga tcctggacgc ctgcaagctg catcccctgt cggtcaacat
     3961 ggcggcgcgc attcacgccg gaagcccgga tgcctacctg cgccgcatcg ccgaggtgta
     4021 tgtcaccgga acgccgatgc ccgagctttt caacgacgac gtctatgtgc cggcgttgcg
     4081 gcacaattac ccggacacca cgattgccca gtcccgcaac tacagcatcg tcggatgcgt
     4141 cgagccttgt gcctcgaccg agcatttcgg caacaccgac agcgcaaacg tgaacctgac
     4201 catgccgctt ctgcaggccc tgacgggaga caccacccgg ctttggcggt acggcggact
     4261 gcgcggcatc gagcgccggg gactggactt cgcggcgaag cgccttgcaa caggtccgct
     4321 gaccgcgcgc atggtcgatc acgtggcgga tcgcctgtca tcacccacat gtgtgaaccc
     4381 gccggcgagc atggacgaac ttctggatcg cttccaggat cgcctgaacg aagtaaccgc
     4441 ggatgtgctg gccgaccagt acctgattga aacggcgctg gcgaaaaccc tgccggtgcc
     4501 cctggcttcc gcgacgttcg aaagcgctgt gacggccggc cgcgacctgc ttcagggcgg
     4561 cgccgccatc aacacgagtg ggatccaggc agtcggcgtg accgacgtcg ccgactcgct
     4621 gtacgcgatt gacgaactgg ttttcaagcg cggcgcattc acgatgcaac agttgctgga
     4681 cgccttggat gccaatttcc aggggccgaa acacgagcgc atccgcgaaa tgctgatggc
     4741 aatcccgaaa ttcggcaacg acgcggatcc ccgaccggcc cagtgggaaa cccgcgtaat
     4801 ggaagcctgg tgcaaggctc tggccgcagt tccgaagacg acccgcggcg ggcgttatgt
     4861 ggccggatac tacggcctga acgtgaacct ggcgtacgga aggaacaccc ccgccctgcc
     4921 cagcggacgt ctggccggcg ttcccctggc aaacagcgtg gtgccgcact atgggatgaa
     4981 gatggttgac ctgaccggat ccctgaacgc ggttgcccag gtcgatttcc cgaataacgc
     5041 cccgaacggc accacgaaca cggcgaccat cgacgcggcg ctgttcccgg gggacggcgg
     5101 catcgacaac ctggccggca tgatccgcgc ttacttcgac cagggcggga tgcagttcca
     5161 gccaaacgtc ttttcacgcg agacgttgct ggacgcttac aagcacccgg aaaagtatcc
     5221 gaacctgctt atccgcatcg ccggctactg cgcttacttc aacgaccttt ccgacgacct
     5281 gaagctggaa atcatcaacc gcacgtacta cagcgacgcg gtctgattcc tccgattgaa
     5341 cattcggaca gatttgacgg gaacttaagg cctagaaatc cttgggaacc ttgacggtgg
     5401 ccggtttgcg gacgcggaag gtgttttcga cggccgtcag catttcaatc agggcgtctt
     5461 caaacttcat cgtttcaagg gcttcgccgg tcgcgccgta acggttcgtg gcctgattga
     5521 cgaagaagtg gccgctgtta cattcctgtt cgtgattcgg gttgaactcg ctcttgggga
     5581 tgttggtaac cgtgcacgtt tcgtgcggcc ccttgcattc gttgtcaacg cacgtcatca
     5641 catcgctgtt ttcgaagcaa ttgcagacct gggctccggt ggtcttgttg cggaaacagc
     5701 actgtccgtc cggaacgatc aggatgtaac gcggactgct gccgtatccc atggtcaggt
     5761 tgaacagcat ggtaaggatg cccatgccgt ccaggggcat gccgatacct ggcagcatgt
     5821 aggaaaccgg gtattcaagt tcgttgtaca gaaccttgtc ttccggattt tccgcggtgc
     5881 aattcgcata gcagtcggtg ctgtcgtaat ccggcggaag gttgaccgac atccagcgac
     5941 ggctcttcag aacttccgag tagtacgagg tgaagaagat ctcgcccagg aaattgtagt
     6001 tcaacttcac gaccggatcg gggatgcgga cgttggcggc ggcgtacggc accaggccgc
     6061 gcagattgac ttcacaaccg tttccgatgt ggtttccgct gccgtcgatg tacgcatctt
     6121 cctcgtccat gtctggatcg ccgtaagcaa tcaggaagct gttgccaaag gacttgcgcg
     6181 aaaagtccac ggtgccgaag tcttccttcg ttccaagcaa ctgcttgcat tccagatccg
     6241 ggaacacaga atcttcaaac ccggtgaaat tcttgtaata acgaatatca acgccgcgat
     6301 ctttcagcgt gttggccagg tgcagcgcgg atgtcagggc gcccaggccg tcccttattc
     6361 caccgtcaaa gaaatagttc ttcttctgca gatcctccag tggcgtcttc ttgaagaagg
     6421 cgcgggcgtc gttttcgatc agg
//
LOCUS       DAOPPA010000142         6436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13187_length_6436_cov_3.674659, whole genome shotgun sequence.
ACCESSION   DAOPPA010000142 DAOPPA010000000
VERSION     DAOPPA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13187_length_6436_cov_3.674659"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKH54_05745"
     CDS             complement(<1..870)
                     /locus_tag="PKH54_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99426.1"
                     /translation="MKKIPGIIAAVLLLALAGTPAAAQEAFDGTQWPPPVNESEDDQF
                     PGAEDGVPPATDTDEVPPAPEEDVPPAPEEDEGSDSDMNTSSSGNWTDKITFSGYVSS
                     DISFIVEDWRGPTPGDGYRFDTNRNDVNFKLKIDPNDNVGIVTDMRFRFYGLYDGINL
                     VDLSKRGPIDPWDFQLNEAYVQVKGKPWSKMDFKIGRVMETWGTADMFNPTDNLNARD
                     FSDPLDFFAKVPNSMVIVNIYPTDWLTITAAWLPIFKPAMLPRSAKNAFALPRNRAGQ
                     VTGFPAMPMDTEDM"
     gene            complement(873..1607)
                     /locus_tag="PKH54_05750"
     CDS             complement(873..1607)
                     /locus_tag="PKH54_05750"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOC99427.1"
                     /translation="MKTVANLVMAIVVVTVPFLATASEPVDSVFAKMDKVTNGYDDSM
                     MDVHMTIYNLDGSKKTYDCEIYQKGLKRLLKFTSGEIKGMANLIMSPSEVYAYLPSQK
                     KIRRVAPHKMGQSLAGSDMTSDDAAFASWVEVYEPKLEKEDDKFWYVYATGKKGAPYP
                     AARLKIQKGDYLLAEYQFLNASDGLVKTYTCTDVRNWSGIKRARHITIQDGQSAHKTT
                     LELKKFKVNQGLKDDFFSKRQLEWGR"
     gene            1968..3125
                     /locus_tag="PKH54_05755"
     CDS             1968..3125
                     /locus_tag="PKH54_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC99428.1"
                     /translation="MIDERRRDSRRPLDYDVTCYIDGARLDAHALNVSQRGAFVGTEL
                     GRPVPVGALAGLVFISDSTSVGTTFLFGRVIRQQDVPIRGFAVRWEKAVSAGRTNDLK
                     SFLNQFLGVEDAVVEVELVGPSGSSSQMSRCVYRFEPFDPAAADAPGNQDIVDSIGYG
                     RRVIRDSMADPSPDPFAVTGLENVLDPISIEVEFESDQHAGPAGFSSAPHSSRHRSPG
                     RSRQGRAGMGELESFIGNLDVSEEEISAVRIGGHASPIHLPPAAVVGPAVLGALPDVL
                     SVPDSVACEMEGAVIVAGSKLDVVVTGLSMGAAVVRSRFMPIDPDSGLILELTIGSRK
                     GVSTVLCHGRVTGLQRGAESGFVVTFGKIDEGESVGIFERYLKWLKFNGAV"
     gene            complement(3154..3978)
                     /locus_tag="PKH54_05760"
     CDS             complement(3154..3978)
                     /locus_tag="PKH54_05760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC99429.1"
                     /translation="MYEKRTHFRRPLKVPVTCFVEGRKFEAEARDISRGGIFLETRDS
                     STIPMSAVVGLMFPGDARDQSTFLFGRVARKEDGSHEGVGLSWEKAVSGGNADGLASL
                     LTKMFGISNPKIQVERNHRGERSVFKFDCETSRLNDAKPAGQPRIQAATSRDQERSTH
                     GSPGSSTDGDSVSCYIRGYLEENLVRYPALITRLGPRSALAHTTLKYDPRPMYADLIF
                     SIPSKEGPVEVICKSEARQISAEEPGTVAFRFFSIEEGESRGLLRRYLAWLQLHQN"
     gene            complement(4002..4751)
                     /gene="gpmA"
                     /locus_tag="PKH54_05765"
     CDS             complement(4002..4751)
                     /gene="gpmA"
                     /locus_tag="PKH54_05765"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOC99430.1"
                     /translation="MYKLVLLRHGESQWNLENRFTGWTDVDLSPTGVNEAIEAGRVLK
                     AEGFEFDIAYTSVLKRAIKTLWLALEQLDQMWIPVVNHWRLNERHYGALQGLNKAETA
                     QLHGEDKVKVWRRSYDIPPPSLESADPRFPGNDRRYSGLSQEELPLTESLKLTVDRFV
                     PYFEGTIAPMIKSGRRVIIAAHGNSLRALVKYLDGISDADITELNIPTGIPLLYELDE
                     NLRPISSRYLGDQEKIAGKIQAVANQAKKKA"
     gene            complement(4849..>6436)
                     /locus_tag="PKH54_05770"
     CDS             complement(4849..>6436)
                     /locus_tag="PKH54_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOC99431.1"
                     /translation="EYVHSFGSRVYVTLNIPVTRLNAKETARTMASCHVSGVDALIVR
                     DPIIMKVAQDLVPDLPIHASTQAGVHSVETARRMADLGCRRVILARECSRSDIAAIRR
                     ALPELELEVFVFGAMCFGVSGLCMMGHAVSGRSGNHGACCQSCRLPYFDTENKPVGYV
                     FSMKDLDLVPHIPELVDMGVAAFKIEGRLKTPSWVGCVTKWLRTALDRPTPGLEPQEY
                     DQFDRDISVLYSRKRTDGFFVGKTDAGTVLSTDNQTHAGLDVKVFKCRQDHTGHWVSF
                     RTPVDINVRDGLLLKIRDDSAPGDCRWIPAGIRALVDGRGRSAIRIAAGHDVLIPLPQ
                     EMDHAVVLAVHIHSADSIRARYRKVERPVPGAVTEGTLPWPEFLDVEVTPNSISARMR
                     LGRFTASAACPVESFPARSDGISPEHMKKYFGDAETTIAPGLFINPSSMKAARRELVA
                     GFMSARDEETGRQAHAIGTEIAAMSDQFLPSDDELIQRGNGTLAISRVTGMKEGCVYT
                     SGGDGLVIEPGPDSTRIRKA"
BASE COUNT         1258 a         1788 c         1813 g         1577 t
ORIGIN      
        1 catgtcctcg gtgtccatcg gcatggcggg gaaaccggtg acctggccgg cgcgatttct
       61 tggcagggcg aaggcgttct tggccgaacg gggcagcatc gcgggcttga agattggcag
      121 ccacgccgcc gtgatcgtca gccagtcggt cggatagatg ttgacgatga ccattgaatt
      181 cggaaccttg gcaaagaaat caaggggatc cgaaaagtcg cgtgcgttca ggttgtcggt
      241 cgggttgaac atatcggccg ttccccacgt ttccatgacg cggccgatct tgaagtccat
      301 cttgctccag ggcttgccct tgacctggac gtacgcttcg ttcagctgga agtcccacgg
      361 gtcgatcggt ccgcgctttg acagatccac caggttgatg ccgtcgtaca gtccgtaaaa
      421 cctgaacctc atgtcggtca cgatcccgac gttgtcgttc ggatcgatct tcagcttgaa
      481 gttaacgtcg ttgcggtttg tgtcgaagcg gtatccgtcg ccaggtgtcg ggccgcgcca
      541 gtcttcgacg atgaagctga tgtccgaact gacatacccc gaaaaggtaa ttttgtcggt
      601 ccagttaccg gatgacgacg tgttcatgtc ggaatccgat ccttcatcct cctccggtgc
      661 cggcggaaca tcctcttccg gtgccggggg aacctcatct gtgtctgtgg ccgggggaac
      721 accgtcctcg gcgccaggga actggtcgtc ttccgattca ttgaccggag gcggccattg
      781 agtgccgtcg aaggcttcct gagcggctgc cggtgttccg gccagtgcca gcagaagaac
      841 tgccgcaata attccgggaa tctttttcat gttcaccgac cccattcaag ctggcgcttg
      901 ctgaagaaat cgtctttcaa gccctggttg accttgaact tcttcagttc gagtgtcgtc
      961 ttgtgcgcgg actgtccgtc ctggatcgtg atgtggcggg cccgcttgat tccactccag
     1021 ttcctgacgt cggtgcatgt ataggttttg accaggccgt cgcttgcgtt caggaactgg
     1081 tattcggcaa gcaggtagtc acctttctgt atcttcaggc gtgcggccgg ataaggcgcg
     1141 cccttcttcc cggtggcata gacgtaccag aacttgtcgt cttccttttc cagcttcggc
     1201 tcataaactt cgacccagct tgcgaaagcg gcatcgtcgc tggtcatgtc cgatccggct
     1261 agtgactgtc ccatcttgtg cggggcgacg cgtcggatct tcttctggct tggcaggtaa
     1321 gcatagactt cggacggact catgatcagg ttggccattc ccttgatttc gccggatgtg
     1381 aacttcagca gtcgcttgag tcccttctgg tagatttcac agtcataagt ctttttggaa
     1441 ccgtcaaggt tatagatagt catgtggacg tccatcatgc tgtcgtcata accgttggta
     1501 actttgtcca tcttcgcgaa cacggaatcg accggttctg aagctgtggc aagaaatgga
     1561 accgtcacaa caacgatcgc catgaccaga tttgctaccg tcttcatctg aatatctcct
     1621 tctggaaaac aggtcggaat gttgctctag ccagaatcgg gccaaaattg cagcccaatt
     1681 tgattttgcc ataaaatcca gcctaatcgt acgtttagat ggtatcaggg attccagtca
     1741 tggcagaatg cttgcgatat ctgtcgaaat tggtgcgaag acgcacagca gttcggttgg
     1801 atccgtcgcg gcgtgcgtat tcgcacatat tttggttcaa ccttgtaccc gttgatgttt
     1861 cgggctggtg cccgtccggg gtttgatatt gtttgtaatc gaccgctcaa ccgtgattga
     1921 atactgcggt cgcaggtata aacttcttca ttatcggggt gtcgattgtg attgatgaga
     1981 ggcgcaggga cagtcgccgt ccgcttgatt acgatgtcac ctgttacatc gatggtgcac
     2041 ggcttgacgc acacgctttg aacgtcagtc agcggggcgc gttcgttgga accgagctcg
     2101 gtcgtccggt tcctgtcggt gcgcttgccg ggctggtctt catatcggat tcaacaagcg
     2161 ttggaaccac tttcctgttt ggtcgggtaa tccgccagca ggacgttccc atccgcggtt
     2221 ttgccgtcag gtgggaaaag gcggtgtcag ccggccgcac caacgacttg aaatcattcc
     2281 tgaatcagtt tctgggtgtc gaggacgccg ttgttgaggt ggaactggtt ggcccatccg
     2341 gttccagttc gcagatgtcc aggtgcgttt atcggttcga accgttcgat ccggcggcgg
     2401 cagatgctcc cggcaaccag gatatcgttg attcaatcgg ttacggtcgg cgggtcataa
     2461 gggattcaat ggccgatccg tcgcccgacc cgttcgccgt gacagggctt gaaaatgtcc
     2521 ttgatccgat ctcgatcgaa gtggagttcg aatccgacca gcatgcgggc cctgcaggat
     2581 tcagttcggc cccccactcg tcgcggcacc gctccccggg tcgttccagg cagggcaggg
     2641 ccggtatggg cgaactggaa tccttcatcg gaaatcttga cgtcagcgag gaggagatct
     2701 cggctgtcag gattggcggt cacgcatctc ccatccatct tccgcccgcg gccgttgtcg
     2761 ggccggccgt tctgggtgcg cttcccgatg tcttaagcgt tcccgattca gtcgcctgcg
     2821 agatggaggg cgccgtgatt gtggccggtt cgaagctgga cgtggtggtc accgggcttt
     2881 ccatgggcgc agccgtcgtc cgatcccggt tcatgccgat tgatcccgat tccggtttga
     2941 tacttgaact taccataggg tctcgcaagg gtgtttcaac cgtcttgtgc catggtcgcg
     3001 ttacaggtct tcagcgtggg gccgaaagtg gtttcgtggt gacgttcggc aagattgacg
     3061 aaggtgaatc tgtcgggata tttgagcgtt acctgaagtg gctgaagttc aacggcgccg
     3121 tctaggccga tcaggttgaa agaatccggc cgatcagttc tggtgaagct gcaaccatgc
     3181 cagatatctt cgaagcaggc ctcttgattc accttcctcg atcgaaaaga accggaacgc
     3241 aacggtgcct ggttcctcgg cgctgatttg gcgcgcctcg ctcttgcaga tgacttcgac
     3301 gggaccttcc ttggacggaa tggaaaagat cagatcggca tacattggac ggggatcgta
     3361 cttgagtgtg gtgtgtgcca gggcagatcg cggcccaagc ctggttatca atgccgggta
     3421 acggacaagg ttttcttcca gatatccccg gatgtagcac gagaccgagt ccccgtctgt
     3481 cgaactgcca gggcttccgt gggtcgatct ttcctggtcg cgactggttg cagcctgaat
     3541 ccttggctgg ccggccggct ttgcatcgtt aagcctggac gtttcacaat cgaatttgaa
     3601 aacgcttctc tcgccacgat ggttgcgctc aacctgaatc ttcgggttgg aaatgccgaa
     3661 catcttcgtc agaagggatg cgagtccatc cgcgttgccg ccggataccg ctttttccca
     3721 tgagagtccc acaccctcgt ggctcccgtc ttcctttctt gccactcgac cgaacaggaa
     3781 ggttgactga tcccttgcgt cacccggaaa catcagaccg accacggcgg acattggaat
     3841 cgtggaagag tcccgtgttt caaggaatat gccgccccga ctgatatccc tggcctcggc
     3901 ttcgaatttt cttccttcga cgaaacatgt gaccggaact ttcagaggtc ttctgaaatg
     3961 cgttctcttt tcatacatgc acgaaagcgc ttcttcaaca atcaggcctt cttcttcgcc
     4021 tgattcgcca cggcctgaat cttgcccgcg atcttttcct ggtcgccaag gtaccggctg
     4081 gagatcgggc gaagattttc atcaagttcg taaagcagtg gaatcccggt tggaatgttc
     4141 agctcggtga tgtcagcgtc cgagatgccg tcaagatatt tgacaagcgc ccgcaggctg
     4201 ttgccgtggg cggcaatgat cacgcgccgg ccggacttga tcatcggagc gatggtgcct
     4261 tcgaagtacg gaacaaagcg atcgaccgtc aacttcaggc tttccgtcag gggcagttct
     4321 tcctgagaaa gtccggagta tctgcgatcg ttgccaggga aacgcggatc ggcgctttca
     4381 agcgatggcg gcgggatgtc atagctgcgt cgccagacct tgaccttgtc ctcgccgtgc
     4441 agttgagcgg tttcggcctt gttcaacccc tgcagggcgc cataatgtct ttcgttcagc
     4501 ctccagtggt tcacgacggg aatccacatc tggtcaagct gttcaagggc aagccacagt
     4561 gtcttgattg cccgtttaag gacggacgta tatgcgatgt cgaattcgaa gccttcggcc
     4621 ttcagtaccc tgccggcttc gatcgcttca ttgacgcccg ttggcgacag gtcaacatcg
     4681 gtccatccgg tgaatctgtt ttcaaggttc cactgacttt caccgtgtcg aagaagcacg
     4741 agtttgtaca tgattccttc ccccaaccca ttattgacgg gttgtttgcg caaaagtcta
     4801 tcagcgaatg gcggcaactg ccaaaaaacc acgcattatc acgaaatatc aagcttttcg
     4861 gattctggtc gaatcgggcc ccggttcaat tacaaggccg tcacccccgg aagtatagac
     4921 gcaaccttcc ttcattccgg tcacgcgtga aatggcaagg gttccatttc cccgttgaat
     4981 aagctcgtcg tccgaaggca ggaactgatc cgacattgca gcgatttcag ttccgatggc
     5041 atgtgcctgc cgacctgttt cctcatcacg tgccgacatg aaaccggcaa caagttcgcg
     5101 tctggctgcc ttcatgctgc tcggattgat aaacagtccg ggcgctattg tcgtttcggc
     5161 atcaccaaag tatttcttca tgtgttcggg cgaaatgcca tccgatcttg ccggaaaaga
     5221 ttcgactggg cacgctgccg aagcggtgaa tcgtcccagt cgcatccgag cgcttatgga
     5281 gttgggtgtg acctcgacat caaggaattc cggccatggc agggttcctt cggtgacggc
     5341 gccgggcacc ggtctttcga ccttcctgta tcttgcccgg atggaatctg ccgaatggat
     5401 gtgtactgca agtacgacgg catgatccat ttcctgggga agcgggatca agacgtcgtg
     5461 accggcagca atcctgatgg ccgaacgccc cctgccatcg accagggctc gtattcccgc
     5521 gggaatccac cggcaatcgc ccggggccga atcgtccctg attttcagca gaagtccgtc
     5581 cctgacgttg atatccactg gcgtcctgaa cgatacccag tgacctgtgt gatcctgtct
     5641 gcatttgaat actttgacgt caagtccggc gtgtgtctgg ttgtcggtcg aaaggacggt
     5701 tccggcgtcg gtcttgccaa caaagaaccc gtcggttcgt ttgcgtgaat aaagcaccga
     5761 aatgtcacga tcgaattgat cgtattcctg cggctcaagg cccggcgttg gcctgtcgag
     5821 ggcggtccgc agccatttcg tgacgcatcc gacccatgac ggggtcttca gcctgccttc
     5881 gatcttgaat gccgccaccc ccatatcgac cagttccggg atgtggggaa ccaggtccag
     5941 atccttcatg ctgaaaacgt aaccgactgg tttgttttcc gtgtcgaaat acgggagacg
     6001 gcatgactgg cagcacgcgc catggttacc tgatcgcccc gaaacggcgt gccccatcat
     6061 gcacaggccg gatacgccga aacacatcgc gccgaaaacg aacacctcca gttcgagttc
     6121 cggcagcgcc cttctaatcg cggcgatgtc gcttctcgaa cactccctgg caaggatgac
     6181 ccttcggcag cccagatccg ccatccgtcg ggcagtttca accgaatgaa ccccggcctg
     6241 ggtgcttgcg tggattggaa gatccggcac caggtcctgg gcaaccttca tgatgatcgg
     6301 atccctgact atcagggcat cgacaccgga gacgtggcat gacgccattg tcctggcggt
     6361 ttcctttgca ttcaagcgcg tgacagggat attcaaagtg acgtaaaccc tggatccaaa
     6421 cgaatgtacg tactcc
//
LOCUS       DAOPPA010000143         6377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13350_length_6377_cov_4.541443, whole genome shotgun sequence.
ACCESSION   DAOPPA010000143 DAOPPA010000000
VERSION     DAOPPA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13350_length_6377_cov_4.541443"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PKH54_05775"
     CDS             complement(<1..989)
                     /locus_tag="PKH54_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOC99432.1"
                     /translation="MKRFLILAVIGVAGFAGCAEKSFDELCPNQQMCVLKCPSGTKQR
                     VEGLQILCTNSAGVHHGPFVVWNADGGKDMVGSNVDGKMDGPVMSWHPNGVLKLQAGF
                     AAGKKDGLWTTWDELGNKVLEGTFAADERDGVWTAWHADGTRKGQSVYKDGLKDGIWE
                     SWHENGRKASEGRFERDLKVGMWTYYYDSGQKKSESNYVDNRENGTVTSWYENGNVQV
                     SGQAAMGLREGVWKGFFEDGRPKYEKTFVKGSENGAYREFHPGGGIAVEGTHKDGKLE
                     GLLKFFAPDGRNLLVMNYRQGKLQGRSIGYSIDGKVRIEVFYEGGVLAGAPKA"
     gene            complement(1030..1869)
                     /locus_tag="PKH54_05780"
     CDS             complement(1030..1869)
                     /locus_tag="PKH54_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC99433.1"
                     /translation="MRIHFIGDISKDFNVIRGQTREEPGGGVFHNSMTAAAIGADVVA
                     VITCAPEDRGITAPIAAKNVPIVFCGSGRSTTTIENVYPDENPDSRRSRLIAGATPFE
                     PGDFDAVSGGVIHVNPLWYPLFSMDILAGLRQKAELLAADAQGFLRNIAADGSMYLKV
                     PPDVERGLALLDVLKVDIKEAAALTGIDDVEPAARAVRAMGPRIVVLTHADGVCVFDG
                     SRIYEAPFGKYPMEGRTGRGDTCSAAFLCALARGGDTAAATEFAGRITSSKLQYPGPY
                     RGD"
     gene            complement(1912..2937)
                     /gene="iolG"
                     /locus_tag="PKH54_05785"
     CDS             complement(1912..2937)
                     /gene="iolG"
                     /locus_tag="PKH54_05785"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOC99434.1"
                     /translation="MKKLTIGVIGAGRIGKVHAKTISKLVIPGVDVRIKWVVDPVVKM
                     AEELAAALAIPKSGADYREVLADPEVDAVLVCSSTNTHSEISIAAAKAGKHVFCEKPV
                     DLTIARVDAAIAAAKEAGVILQVGFNRRFDPDFRKIRTIVASGAIGVPHILKITSRDP
                     NPPSPQYAAVSGGMFLDMTIHDFDMARYLIGSEVAEVFAAGAVLVDPEIGRAGDIDTA
                     LITLRFENGTIGVIDNSRKAVFGYDQRAEVLCSNGMAANSNHYHDTVVVSGAERIEND
                     LPQNFFMDRYTLSFEVEIGEFVEAILGGKAPSCDGRDGRQALVIALAAKKSLVTGRPV
                     KTAEVEA"
     gene            complement(2955..3878)
                     /locus_tag="PKH54_05790"
     CDS             complement(2955..3878)
                     /locus_tag="PKH54_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC99435.1"
                     /translation="MSNPIKVANAPCSWGALEFDLDGQAAGADRVLSEMSRAGFVGTE
                     FGDWGFLPTDKDGLKAILDKYGLQMIGAFVPVALWKREAWEKGIETAVRTANLLASVA
                     ERPFIVLADENGSVPERTKNAGRIRPEQGLTDEQFKTFGEGANALARAVLEMTGVKTV
                     FHHHCAGYIETPAEVEKLLSVTCPDLLGLCLDMGHYRFGGGDPVEALKKHADRIWHIH
                     YKDCSVEVAERARREGLDYFQAVAAGVFCELGKGDVDFAAITKILNERNAAGRLPAID
                     WIVVEQDVLPGMGSPFESAVRNRQYLKTLGL"
     gene            complement(3884..5749)
                     /gene="iolD"
                     /locus_tag="PKH54_05795"
     CDS             complement(3884..5749)
                     /gene="iolD"
                     /locus_tag="PKH54_05795"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HOC99436.1"
                     /translation="MNTTRMTTAQAIIAFLKNQYVDRDGAQQRFFGGVFGIFGHGNVA
                     GIGQALQENPDFPYFQSKNEQAQVHLAAAYARMKNRMGAMVCTSSIGPGATNMITGAA
                     AATINRVPVLLLPGDIFARRNVAPVLQQVESDATQDISVNDCFKPVSRYWDRIVRPEQ
                     ILTSLPEAMRVLTSPAQTGAVTLSLCQDVQAMAYDFPTAFFRRRVWRIGRQLPDAGFT
                     VDAADMIRQARRPFIVAGGGVIYSDACNALARFCEMTGIPVGETQAGKGSLRWDHPQS
                     LGGIGATGTAAANVAAREADLVIGIGTRYSDFTTASKTQFENPDVHFININVCEFDAA
                     KHDGLMLVGDAAATIHALGDLLEDWSVDPLWAARTAQMKKAWDAEVERIYHLGHGPLP
                     SQGEVIGALDEFLGDRDVIINAAGSAPGDLHKLWRARDCKQYHLEYGYSTMGYEIPAA
                     IGVKMAAPDRDVYAIVGDGTFLMMPSEIATAVQEGVKVTYIVIDNHGFASIGGLSASL
                     GSGGFGTRYRMRTEAGLDDGAPLPIDFEAICRGFGAHAVRVADIAGFKAALASTRDVK
                     GPVAIVIETDREARVGGYESWWDVAVAEVSGMEAVGAARAKYVENVRKERNFFTE"
     gene            complement(5764..>6377)
                     /locus_tag="PKH54_05800"
     CDS             complement(5764..>6377)
                     /locus_tag="PKH54_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOC99437.1"
                     /translation="FQQGDKSHTATRTSLFSQDPYCMHVPAGCPVTIRALADCELALF
                     RVTNDRSFSPRFYDPKTMLECELRDRGRWNDTAYRVVRTIFDNRNNPDAQLVLGEVVN
                     FGGRWSSYPPHHHPQPEIYHYRFSMPQGYGHGELGDDVLKIRQYDTLRISGGNDHSQV
                     AAPGYEMYYIWAIRHLPGNRYTTPEFTAEHVWLKDLPFTGTQF"
BASE COUNT         1153 a         2215 c         1760 g         1249 t
ORIGIN      
        1 caggccttcg gggcacccgc aagcacgcca ccctcgtaaa agacctcgat ccttaccttt
       61 ccgtcgatcg aatacccgat cgatcggccc tgcagcttgc cctggcggta attcatgacc
      121 agcaggttgc gtccgtccgg cgcgaaaaac ttcaacagcc cttccagctt gccgtccttg
      181 tgcgttccct cgaccgcgat tccaccgccc gggtgaaatt cgcggtacgc gccgttttcg
      241 ctgcccttga cgaaagtctt ttcgtacttc ggccgcccgt cctcgaagaa gcccttccag
      301 accccctcgc gcagacccat cgccgcctgg cccgaaacct ggacgttccc gttttcatac
      361 caggatgtta ccgtcccgtt ttcacggtta tcgacataat tcgattcgga cttcttctgg
      421 ccgctgtcgt aataatacgt ccacatcccg accttcaggt cgcgctcgaa ccggccttcg
      481 gacgccttgc gtccgttctc gtgccacgat tcccagatcc cgtccttcaa cccgtcctta
      541 tagaccgact gcccctttcg cgtgccgtcg gcgtgccagg cggtccatac cccatcccgt
      601 tcgtccgcag caaacgtgcc ttcaagaacc ttgttcccca gctcgtccca cgtcgtccac
      661 aacccgtcct tctttccggc cgcgaacccg gcctgcagct tcaaaacccc gttcggatgc
      721 cacgacatga ccgggccgtc catcttgcca tccacattgc tgccgaccat gtccttcccg
      781 ccatcggcgt tccagaccac aaagggaccg tggtgcacgc cggccgaatt ggtgcacaga
      841 atctgcaatc cttcgaccct ctgcttcgtc cccgacgggc acttcaacac gcacatctgc
      901 tggtttggac aaagctcgtc gaaagacttt tcggcgcacc cggcaaaacc ggccacgccg
      961 ataacagcaa gaatcagaaa ccttttcatc acatttgtcc cttgcacgac catcggccgc
     1021 gtgcccccat caatcgcccc ggtacgggcc gggatactga agcttactcg acgtgatgcg
     1081 gcccgcgaat tcggtggccg ccgccgtgtc cccgccacgc gccagcgcgc acaggaacgc
     1141 cgccgagcac gtgtccccgc gccctgtccg cccttccatc gggtacttgc cgaaaggcgc
     1201 ttcgtaaatc cggcttccgt caaaaacaca gacgccatcg gcatgcgtaa gcacgacaat
     1261 ccgcggcccc atcgcccgca cggcccgcgc cgccggctcg acatcatcga tgccggtcaa
     1321 cgccgctgcc tccttgatat cgaccttcag aacgtcaagc agggcaagcc cacgctcgac
     1381 atcgggcgga accttcaggt acatgctgcc gtccgccgcg atgttgcgca aaaagccctg
     1441 ggcatccgcc gccagcagct cggccttctg ccgcagcccg gccaggatgt ccatcgaaaa
     1501 cagcggatac cacagcggat tgacgtgaat gacgccgccc gaaactgcgt cgaagtcgcc
     1561 gggctcaaaa ggcgtggccc ccgcaatcag ccttgaccgc ctggaatccg gattttcatc
     1621 cggatacacg ttttcgatgg tcgtcgtcga tcgaccggac ccgcagaaca caatcggcac
     1681 gttcttcgcc gcgatcgggg ccgttatccc gcgatcctcc ggggcgcacg taatcaccgc
     1741 gaccacgtcc gcgccaatgg cggccgccgt catcgagttg tggaacacac cgccgcccgg
     1801 ctcttcccgg gtctggccac gtattacgtt gaaatccttt gaaatgtcgc cgatgaaatg
     1861 aatgcgcatc tttccccttc cacggccggg atcgacgcgc cgcgcgcccg atcaggcctc
     1921 gacttcagcc gtcttcacag gccggcccgt aaccagcgac ttctttgccg ccagcgcgat
     1981 gaccagcgcc tgccttccat ccctgccgtc gcaggacggc gccttgccgc caagtatagc
     2041 ctcaacgaac tcgccgatct caacttcaaa ggacagcgta taacgatcca tgaagaaatt
     2101 ctgcggcagg tcgttttcga tcctttcggc acccgacacg accacggtgt cgtgataatg
     2161 gttggaattg gccgccatgc cgttgctgca aaggacctcg gcgcgctgat cgtaaccgaa
     2221 aaccgccttg cggctgttgt cgatgacgcc gatcgtcccg ttttcaaagc gcagcgtgat
     2281 cagcgcggta tcgatgtcgc cggcgcgacc gatttcagga tcgaccagca ccgcaccggc
     2341 cgcgaacact tccgccactt cgcttccaat caggtacctg gccatgtcga aatcgtgaat
     2401 cgtcatgtcc aggaacattc cgccggaaac ggccgcgtac tgcggcgacg gcggattcgg
     2461 gtcgcggctg gtaatcttca ggatgtgggg cacgccgatg gcgcccgacg ccacgatggt
     2521 tcgaatcttg cggaagtccg ggtcgaagcg gcggttgaaa ccgacctgaa ggatcacgcc
     2581 ggcttccttc gccgccgcga tggccgcgtc aaccctggcg atggtcagat cgaccggctt
     2641 ttcgcagaac acgtgcttgc ccgccttcgc cgcggcaatc gaaatttcgc tgtgggtgtt
     2701 ggtgctgctg cacaccagca cggcatccac ttccgggtcg gccagcactt cacggtagtc
     2761 ggcgcccgac ttcgggatgg caagggccgc ggccagttcc tcggccatct tcaccacggg
     2821 atccacgacc cacttgatcc ttacgtcaac accggggatg accagcttgg aaatcgtctt
     2881 cgcatgaacc tttccgatac gtccggcacc gatgacaccg attgtcagct tcttcatttt
     2941 tcgtccttcc ttcatcaaag accaagggtc ttcaggtatt gcctgttcct gaccgcgctc
     3001 tcaaacggcg agcccatccc gggcagcacg tcctgctcga ccacgatcca gtcaatcgcc
     3061 ggcaggcgtc cggccgcgtt gcgttcgttc aggattttcg taatcgccgc gaaatccacg
     3121 tcgcccttgc cgagctcgca gaacacgccg gccgcgaccg cctggaagta atccagtcct
     3181 tccctgcgcg cgcgctcggc cacttcgacg ctgcaatcct tgtaatggat gtgccagatg
     3241 cggtcggcat gcttcttcag tgcctcgacg ggatcgccgc cgccgaagcg gtaatgtccc
     3301 atgtccaggc aaagccccag cagatccgga caagtgacgg aaagcagttt ttcgacctcg
     3361 gccggcgtct cgatataccc ggcgcaatgg tggtgaaaca ccgtcttgac gccggtcatt
     3421 tccaggactg cccgcgccag ggcgttcgcg ccctcgccaa acgtcttgaa ctgctcgtcg
     3481 gtcagtccct gttccgggcg gatgcgtccg gcgttcttcg tgcgttccgg cacgctgccg
     3541 ttttcatcgg caagcacgat gaacggacgt tcggccacgg aagccagaag gttcgccgtc
     3601 ctgaccgccg tctcgattcc cttttcccac gcttcccttt tccacagagc caccggcacg
     3661 aacgcgccga tcatctgcag gccgtacttg tcaagaatcg ccttcaggcc atccttgtcg
     3721 gtcggcagaa aaccccagtc gccaaactcg gtgccgacaa atccggcacg gctcatttcc
     3781 gacagcacgc ggtccgcgcc ggccgcctgc ccgtcgagat caaattccag ggcaccccat
     3841 gaacatggcg cgttggccac cttgatcggg ttcgacatct tgttcactcc gtgaagaaat
     3901 tgcgttcctt cctgacgttt tccacatatt ttgcccgcgc cgcgccaacc gcttccatcc
     3961 cggaaacttc ggccacggcg acatcccacc acgactcgta cccgccgaca cgcgcctcgc
     4021 ggtcggtttc gatgacgatt gcgaccgggc ccttcacatc gcgcgtcgaa gccagcgccg
     4081 ccttgaaccc ggcgatgtcg gccacccgga ctgcatgggc gccgaatccg cgacagatcg
     4141 cttcgaaatc gatcggcagc ggcgcgccgt cgtccagccc cgcttcggtg cgcatccggt
     4201 acctggttcc gaacccgcca ctgcccagcg atgccgaaag cccgccgatg ctggcgaatc
     4261 cgtgattgtc gatcacgata taggtcacct tgacgccttc ctgcaccgcc gtcgcgattt
     4321 cggacggcat catcaggaac gtcccgtcgc cgacgatggc atacacgtcg cgatccggcg
     4381 cggccatctt cacgccgatg gccgccggaa tttcatagcc catcgtcgaa tatccgtatt
     4441 ccaggtggta ctgcttgcag tcgcgtgccc gccacagctt gtgcagatcc cctggcgcgc
     4501 tcccggccgc gttgatgatg acgtcgcgat cgccaaggaa ttcgtccagc gccccgatga
     4561 cctcgccctg tgacggcagc ggcccgtgtc ccaggtgata aatcctttcg acctcggcgt
     4621 cccacgcctt cttcatctgc gccgtcctgg ccgcccacag tggatcgacc gaccagtctt
     4681 ccagaagatc gccaagcgca tgaatggtcg ccgcggcgtc gccaaccagc atcagtccgt
     4741 cgtgcttggc cgcgtcgaac tcgcagacgt tgatattgat gaaatggacg tcgggatttt
     4801 caaactgggt ctttgacgcc gtcgtgaagt ccgaatacct ggtcccgatg ccgatgacca
     4861 ggtcggcttc acgtgccgcc acgttcgccg cggccgttcc cgtcgcgcca atgccgccaa
     4921 gcgactgggg atggtcccac cgcaggctgc cttttcccgc ctgggtctcg ccgaccggaa
     4981 tcccggtcat ttcacagaag cgcgccagcg cgttgcaggc atcgctgtaa atgacgccgc
     5041 cgcccgccac gatgaacggc cgccgcgcct ggcggatcat gtcggccgca tccaccgtga
     5101 acccggcgtc gggaagctgg cggccgatgc gccacaccct cctgcggaag aacgcggtcg
     5161 ggaaatcgta cgccatcgcc tggacatcct ggcagagcga cagcgtgacc gcgccggtct
     5221 gcgccgggct tgtcaggacg cgcatcgcct cgggcaggga cgtcaggatc tgctccggac
     5281 gcacgatccg atcccagtat cgggacaccg gcttgaagca gtcgttgacc gaaatgtcct
     5341 gggtcgcgtc ggactcgacc tgctgaagca ccggggcgac gtttcggcgc gcgaagatgt
     5401 cgccgggcag cagaagcacc ggcacgcggt ttatggtggc cgcggccgcg ccggtaatca
     5461 tgttggtggc gcccggcccg atcgaggacg tgcataccat cgcgcccatc cggttcttca
     5521 tcctggcata cgccgccgca aggtggacct gggcctgctc gttcttggac tggaaatacg
     5581 gaaaatccgg gttttcctgc agggcctggc ctatgccggc cacgttgccg tgcccgaaga
     5641 tcccgaaaac cccgccgaaa aagcgctgct gcgccccgtc gcgatccacg tactggttct
     5701 tcaggaacgc gattatcgcc tgggccgttg tcatcctggt cgtattcatg cgccaacccc
     5761 ctgtcaaaac tgggtgccgg tgaagggcag atccttcagc cagacgtgct cggccgtaaa
     5821 ttccggcgtt gtatagcggt tccccggcag atgccggatt gcccagatgt aatacatttc
     5881 gtaaccgggc gcggcgacct ggctgtggtc gttaccgccc gaaatccgca gcgtgtcgta
     5941 ctggcggatc ttcaggacgt cgtcgccaag ttcgccatgg ccgtatccct gcggcatgct
     6001 gaaccggtaa tggtaaattt ccggctgcgg atgatgatgc ggcgggtaac tggaccagcg
     6061 cccgccgaaa ttgacgactt caccaaggac aagctgcgcg tccgggttgt tccggttgtc
     6121 gaaaatggtg cgcacgaccc gatacgccgt gtcgttccat cgtccccggt cgcgcagttc
     6181 gcactcaagc atcgtcttcg ggtcgtaaaa cctgggcgaa aacgatcggt cgttcgtaac
     6241 cctgaaaagc gccagttcgc agtcggcaag cgcccttatg gtcaccgggc acccggccgg
     6301 cacgtgcatg cagtaaggat cctgggaaaa cagagacgtc cttgtcgccg tgtgggattt
     6361 gtcgccctgc tggaaaa
//
LOCUS       DAOPPA010000144         6352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13408_length_6352_cov_4.401779, whole genome shotgun sequence.
ACCESSION   DAOPPA010000144 DAOPPA010000000
VERSION     DAOPPA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13408_length_6352_cov_4.401779"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKH54_05805"
     CDS             complement(<1..546)
                     /locus_tag="PKH54_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOC99438.1"
                     /translation="MKSRIDQRTVFAVTLGCPKNRVDTEVMLGDMLSSNYALVDSPGE
                     ADVVIVNTCGFLHAAREESLQALKDVSNEMRPGARLVAAGCMIGRFGDLIEAAVPSVS
                     LMVDTLDFNAISKRLEGLPPPPGAFPTSADPRLVTTGSYAYLKVADGCDRRCSFCIIP
                     SIKGGQRSRPIRDLLVEAGALV"
     gene            complement(571..1359)
                     /locus_tag="PKH54_05810"
     CDS             complement(571..1359)
                     /locus_tag="PKH54_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOC99439.1"
                     /translation="MNTLSKIRSFLLLAIAFLAFSGAASGQPAAPGMGGDAQPQPSSR
                     QPAAVPSPSVQASWQSDPVLVQLNSFYEKTTDFKADFRQVVNTKSPKRTFKRSGTVFF
                     KRPGLMRWDYKVPEAVYYVSDGTILWAYDSEDRSAVKMNVKDSDLYSSLGFLSGTSRL
                     ADSFLARVGASDVEGFASVSLVPVDGSDAGNYQSLVLKVNRATGEVVETEITDPMGNK
                     SRIRFQAMTYAPVPASAFEFKVPSGVSVQDLTKPAPASGAPTRR"
     gene            complement(1423..1893)
                     /locus_tag="PKH54_05815"
     CDS             complement(1423..1893)
                     /locus_tag="PKH54_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC99440.1"
                     /translation="MAVQKWDPFFEIDRLQNEMEKIFQSRWPARGNAVATSSCVVPVD
                     IVETKAEVIIKAEIPGIDPKDVEVKVEDGILTISGEKKMEGHVPAESGQEQQEAEFLR
                     IERFYGKFTRSFVVPRYVDAANVAAEYRSGVLTLKLPRRQETQPRRIEVKVAGE"
     gene            complement(2116..3534)
                     /locus_tag="PKH54_05820"
     CDS             complement(2116..3534)
                     /locus_tag="PKH54_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC99441.1"
                     /translation="MRFPNRAGIIVAACLFAVAVPSASSALERPVTLEEAVKAALEHS
                     PKSRMSQLAVEKARDIRNGSLGPMFPNVTIDAGLQYWDDATGMDFLGGDMPSDDELAA
                     AVEPFAALIPEESKATFGKLMNGLSKMRNYQVADQLTASVTIQAVQPLTPLISLAAAW
                     RAYGAAEDAARLEAVAERNNITYEVSQVFFQLMSAIRMTEVARQGVELVQAHLKMAQS
                     FQKADMVGRDDVLRAETALAQISAQYDQGRHGVELARAALNVMMGIPPDEETVPAGDF
                     PDEPPELTITEEDAVERALAARPEQSKLQAQVRMAEAGKAARVGQLVPVIAAVFRYTW
                     FHGSEFQRENSAFIGAVLQWDFWNMGQRWFEMKAADKDLQMARAGMDMARDLITLDVR
                     KAFIDLKTGRSLIASYRKAVESAEENLRVVTKKYEAASATSVEMLDAHTSLNMARANL
                     AVAVNNYYISYANLQRALAGSL"
     gene            3720..4802
                     /gene="dnaJ"
                     /locus_tag="PKH54_05825"
     CDS             3720..4802
                     /gene="dnaJ"
                     /locus_tag="PKH54_05825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOC99442.1"
                     /translation="MPFITHAMGDRDYYNILGVERGADASEIKKAYRKLAIQYHPDRN
                     PDNPEAEEKFKQLAEAYDVLSDPEKRRVYDQYGADGLKGRGFDFRPDDIFSSFMSMFG
                     GGFEDIFGRGGRQGPSRGRDHQTTQSVTLEEAARGVDKELKLRREEVCSTCSGSGARP
                     GTTPETCRTCNGRGRVAHSQGPFSITTTCGTCAGTGRVIREKCDQCRGSGRVTADRTF
                     KIKIPAGIDTGNSIRVAGAGGVGSPGADPGDLYVVISVLEQEKFRREGDDLITELEIG
                     VPHAALGARMTVTDILDEEVPLNIPAGAQPDELLQISGHGMPRLQYHGRGDLWVQLKI
                     KVPMKLSRKERKLYEELRELSGTGGD"
     gene            4802..5629
                     /locus_tag="PKH54_05830"
     CDS             4802..5629
                     /locus_tag="PKH54_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99443.1"
                     /translation="MIPPVKFIRPLSFGFGVESWLALLGGQKVVLRRFFGPALDPGYR
                     LLPDEFTLFRISHPGIQRLLLSGNDDDGAIVYIYEFIEGRSLYDIMRDDARTAIDGSL
                     LATMLRDGLEALSCLHEESPVAPVLHGDVSPGNMVWGSATPGGPSRLTLIDVRGIDAP
                     GNRDQADDAPDDIIVGTLPYIADEVLGGKMLTPAAETWSLAASLLSAALGGSPWRKAA
                     SPAEMLRMRSDVRPEVLLNDAAGIAGLDDATLRTLAAMLSTDPSDRPSASEAIRMLS"
     gene            complement(5696..>6352)
                     /locus_tag="PKH54_05835"
     CDS             complement(5696..>6352)
                     /locus_tag="PKH54_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HOC99444.1"
                     /translation="DQTVAAMRSLGAPIILYEANRKARDSLITKLLSKKTNFAAQVYD
                     RLRFRLVTAEESDIVPVLSYLKDNLFPYNYVVPGQSRNQIINTSALLEAVPSWASDDP
                     GLGARYLYEDADGTGDDPNGFSGSTFRMINFVVELPLRIRELVPRDNPVLVELGTLVY
                     IQVEFQIFDQTTWKGNEVGDNNHEMYKVRQHWEVVRRLVHGSRFDQGSDATWEGKTRW
                     "
BASE COUNT         1279 a         2018 c         1857 g         1198 t
ORIGIN      
        1 gacaagcgct cccgcctcca ccaacaggtc ccgtatcggc ctgctgcgct ggccgccctt
       61 gatggaaggg atgatgcaga acgaacaacg cctgtcacaa ccgtccgcaa ccttcaggta
      121 cgcgtatgaa ccggttgtca caagacgggg atccgccgag gtcggaaacg ctccaggcgg
      181 cggaggcaac ccttcaagcc gcttcgaaat cgcattgaaa tcaagcgtat cgaccatcag
      241 tgataccgac ggaacggccg cttcaatcag atccccgaaa cgcccgatca tgcacccggc
      301 ggcgacaagc cttgccccgg gccgcatctc gttcgagaca tccttaaggg cctgcaggga
      361 ttcctcccgc gccgcatgca ggaatccgca cgtattgacg atgaccacat cggcttcgcc
      421 cggcgaatcc accagcgcgt agttggatga aagcatgtcg ccgagcatca cctcggtatc
      481 gaccctgttc ttcggacaac cgagggtcac tgcgaatacg gttcgctggt caattcgaga
      541 tttcatcagt cacaccaacg gaaaagaggt tcacctgcgg gtgggggcgc cggacgccgg
      601 ggccggcttc gtcaagtcct gcacgctgac gccggacggc accttgaact cgaatgccga
      661 tgcgggaacc ggcgcgtagg tcatcgcctg gaacctgatc ctggacttgt tgcccatcgg
      721 atccgtgatt tcggtttcaa cgacctcccc ggtcgcgcgg ttgaccttca gtacaagcga
      781 ctggtagttt ccggcgtccg aaccgtccac cggaaccagg ctgactgacg cgaaaccttc
      841 cacatccgag gccccgaccc gggcaagaaa actgtccgcg agcctgcttg ttccagacag
      901 gaagccgagg gacgagtaca gatcggagtc cttcacgttc atcttgaccg cggatcgatc
      961 ttccgagtca taggcccaca ggatcgtgcc gtcgcttacg taatagacgg cctccggaac
     1021 cttgtagtcc caccgcatca accccggcct cttgaagaag accgtgcccg agcgcttgaa
     1081 cgtgcgcttc ggcgatttgg tgttcaccac ctgccggaaa tcggccttga agtcggtcgt
     1141 cttctcgtaa aacgaattca gttggacaag aaccggatcc gactgccagg aagcctggac
     1201 ggaaggtgac ggcaccgcgg ccggctgtcg cgatgacggc tggggctgag cgtcgccacc
     1261 catacccggt gccgccggct gcccggaggc ggcgccggaa aaggccagga acgcaatggc
     1321 aagcaacagg aatgaacgaa tttttgacaa cgtattcatg gcccggattt tacgtgttcc
     1381 gtcggccttt ggccacacaa aaatccggaa ccgaagccgc cgtcattcgc cggcaacctt
     1441 gacctcgatc ctccgtggct gggtttcctg gcgcctgggg agtttcaggg taagcacgcc
     1501 ggatctgtac tcggccgcga cgttcgccgc atcgacatac cttggaacga cgaatgaacg
     1561 cgtgaacttg ccatagaaac gctcgattcg caggaactcg gcctcctgct gctcctgccc
     1621 ggattcggcg ggaacgtgac cttccatctt cttctcgccg ctgatcgtca gaattccgtc
     1681 ctcgaccttg acctcgacat ccttcggatc aatcccggga atttcggcct tgatgattac
     1741 ctcggccttc gtctcgacga tatcgaccgg cacgacgcac gaagaagtcg cgacggcgtt
     1801 gcccctggca ggccaccggg actggaaaat cttctccatc tcgttctgca gcctgtcaat
     1861 ctcgaagaaa ggatcccact tctgaaccgc catgatccac ctcccgcccc gaagggcaca
     1921 ccggcggcgt tcccgcaaac ggtacacgcc accggacaga attgatttgt tatgcgacgt
     1981 ggcgttaagc ctttatcaac aagggcttac gcgcaacgcc cgaaaccggt aaaacaattt
     2041 gtcggactca aagccttgtc aaggggggtc gcgcccgggc gaagccagac acaggcaaga
     2101 tcttgaaatc acttactaaa gcgacccagc caaagctcgt tgcaggtttg cgtaagagat
     2161 gtagtagttg ttaaccgcca cggcaaggtt cgccctggcc atgttcaggg acgtgtgcgc
     2221 gtcaagcatt tcgaccgaag tggcgctggc cgcctcgtac ttcttcgtga ccacccgcag
     2281 gttttcctcg gcggactcaa cggccttcct gtaagaggct atcagcgaac gtccggtctt
     2341 caaatcaatg aatgccttgc gcacgtcgag cgtgatcaga tcgcgggcca tgtccatgcc
     2401 ggcccgggcc atctgaagat ccttgtcggc ggccttcatc tcgaaccagc gctggcccat
     2461 gttccagaag tcccactgaa ggacggcgcc gatgaatgcc gagttttccc gctgaaactc
     2521 gctgccgtgg aaccaggtgt aacggaacac ggccgcgatg accggaacga gctgtccgac
     2581 cctggccgcc tttcctgcct cggccatcct tacctgggcc tgcagtttcg actgctcggg
     2641 cctggccgcc agagccctct cgaccgcatc ttcctcggta atggtaagct ccgggggctc
     2701 atccgggaaa tccccggcgg gaacggtttc ctcgtccggc gggattccca tcatcacgtt
     2761 cagggccgcc cttgcaagct cgaccccgtg acgcccctga tcgtactggg cgcttatctg
     2821 cgcaagcgcc gtttcggccc ggagcacgtc gtcccgaccg accatgtccg ccttctggaa
     2881 ggactgggcc attttcagat gtgcctgaac cagttcgacg ccctgccttg cgacctcggt
     2941 catccttatc gcggacatca actggaagaa gacctgggag acctcataag tgatgttgtt
     3001 gcgctcggcc acggcctcca gacgcgccgc gtcctcggcg gcaccgtacg cgcgccaggc
     3061 cgcggcaagc gatatcagcg gcgtcagggg ctgaaccgcc tgaatcgtca cggaagcggt
     3121 gagctggtcg gccacctgat agttccgcat cttgctcagg ccgttcatga gctttccgaa
     3181 cgtagccttc gactcttccg ggatcagcgc ggcaaaaggc tccaccgcgg ccgccagttc
     3241 atcgtcggag ggcatgtcac cccccaggaa atccatgccc gtcgcgtcgt cccagtattg
     3301 aagtccggca tcaatcgtca cgttcggaaa catcggcccc agcgaaccgt tccggatgtc
     3361 gcgcgccttc tccacggcga gttgcgacat ccttgatttg ggtgagtgtt caagcgcggc
     3421 cttcaccgcc tcctcgagcg tgaccggcct ttccagcgcg gacgacgccg aaggaacggc
     3481 cacggcgaac agacaggctg cgacgatgat cccggcccga ttaggaaaac gcattaaaac
     3541 cccgcaaatc cattaaggcg gccggaaccg gcaagcggca cggccacgcc acggcgcgtc
     3601 attataatac cgggcggccg atatttgacc agcaagtttg tcctgactgg ccggacgccg
     3661 cgcaatcccg cacttttttt ttcgggatcc gcgattcggg attgacacga cgccatcaaa
     3721 tgccctttat tacgcacgcc atgggtgaca gggattacta caacatactt ggtgtcgaaa
     3781 ggggcgccga cgcttccgaa atcaagaagg cttaccggaa gctggcgatc cagtaccacc
     3841 ccgacagaaa cccggacaac cccgaggccg aggagaagtt caaacagctc gccgaagcct
     3901 acgacgtgct gtcggatccg gaaaagcgcc gggtctatga ccagtacggc gcggacggcc
     3961 tcaagggtcg cggcttcgac ttccggccgg acgacatttt ctcttccttc atgagcatgt
     4021 tcggtggcgg tttcgaggat atcttcggaa ggggcggacg ccagggaccg tcacggggac
     4081 gcgatcacca gacgactcag tcggtgacac tcgaggaagc cgcccgcggc gtcgacaagg
     4141 aacttaagct tcggcgcgag gaagtctgct cgacctgctc cggcagcggg gcccggcccg
     4201 gaaccacccc ggaaacctgt cgaacctgca acggacgtgg ccgggtcgcg cacagccagg
     4261 ggcctttctc gatcacgacg acatgcggaa cctgtgccgg aaccggacgc gtcatacggg
     4321 aaaaatgcga ccagtgccgc ggaagcggac gcgtgaccgc ggacagaacc ttcaagatca
     4381 aaattccggc cgggatcgac acaggcaact ccatccgggt cgcgggcgcc ggcggggtcg
     4441 gttccccggg ggccgacccg ggcgacctct acgtggtcat ttccgtcctg gagcaggaaa
     4501 agttcaggcg cgagggtgac gaccttataa ccgaactgga aatcggggtt ccccacgcgg
     4561 ccctcggtgc ccgcatgacc gtcacggaca tcctggacga ggaagttccc ctgaacatcc
     4621 ccgcgggcgc gcagccggac gaacttcttc agatttccgg acacggaatg cccaggcttc
     4681 agtatcatgg ccgcggggat ctctgggttc aactcaagat aaaggttccg atgaagctgt
     4741 cccgcaagga aaggaagctt tacgaggaat tgcgggaact gtccggaacc ggcggggatt
     4801 gatgattcca ccggtcaaat tcatccgtcc gctcagtttt ggtttcggcg tcgagtcgtg
     4861 gcttgcattg ctcggcggcc agaaggttgt ccttcgacgc ttcttcggcc ctgccctgga
     4921 tcccggctac agacttcttc ccgacgaatt cacgctgttc cgcatttccc accctggaat
     4981 ccagaggctt ctcctgagtg gcaacgacga cgatggcgca atcgtatata tatacgaatt
     5041 cattgaagga agatccctgt acgacatcat gcgggacgat gcccggactg caatcgacgg
     5101 atcgctgctc gccaccatgc tcagggatgg actcgaggcg ctcagctgcc ttcacgaaga
     5161 gtcccccgtc gcgccggtgc tgcacggcga cgtctctccg ggaaacatgg tctggggttc
     5221 ggccacaccc ggcggaccgt cccgacttac cctgatcgat gtcaggggaa tcgacgcgcc
     5281 cggcaatcgg gatcaggccg acgatgcccc ggacgacatc atcgtcggga cactgccgta
     5341 cattgccgac gaagtccttg ggggaaagat gctgacgccg gcggccgaga cgtggtcgct
     5401 tgccgcctcc ctgctttcgg cggcgctggg cggcagcccg tggcgcaagg cggcgtcgcc
     5461 ggcggaaatg cttcggatgc gttccgatgt gcgccccgag gttctgctga acgatgccgc
     5521 cggaatcgcc gggcttgacg acgcgaccct gcgcaccctg gccgcgatgc tttcaaccga
     5581 cccttcggac cggccctcgg cgtcggaagc gatccggatg ctatcctgaa ccgcaatcaa
     5641 ttattcggga aatgaaggaa ccggcagaat cccgcccgtg gcgcctcgac cttcctcacc
     5701 agcgggtctt gccttcccat gtggcgtcgg atccctggtc gaagcggctg ccgtgcacca
     5761 ggcggcgcac gacttcccag tgctggcgca ccttgtacat ctcgtgattg ttgtcgccca
     5821 cctcgtttcc cttccaggtt gtctggtcaa agatctgaaa ctcaacctga atgtaaacaa
     5881 gcgtcccaag ctcgacaagc accgggttat cacggggaac aagctcccgt atgcgaagcg
     5941 gcagttcgac gacgaagttg atcatcctga aggtggatcc ggaaaagccg ttcgggtcat
     6001 cgccggttcc gtcggcgtcc tcgtacaggt accttgcgcc caggcccgga tcgtcggacg
     6061 cccacgaagg gacggcttcc agcagggccg acgtgttgat gatctggttg cgggactggc
     6121 caggcaccac gtagttgtat gggaaaagat tgtccttcag gtaggacagc accgggacga
     6181 tgtccgactc ctcggcagtg accagcctga aacgcagcct gtcatagacc tgggcggcga
     6241 agttggtctt cttcgaaagc agtttggtaa tcagggaatc ccgcgccttg cggtttgcct
     6301 cataaagaat gatcggcgcg ccaagggatc tcatcgcggc gacggtctga tc
//
LOCUS       DAOPPA010000145         6327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13480_length_6327_cov_3.632972, whole genome shotgun sequence.
ACCESSION   DAOPPA010000145 DAOPPA010000000
VERSION     DAOPPA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13480_length_6327_cov_3.632972"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2364
                     /locus_tag="PKH54_05840"
     CDS             <1..2364
                     /locus_tag="PKH54_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC99445.1"
                     /translation="EALLEKLSFDDAGIFDISEASRGWAEIKTWYEDRGYLFADVVMD
                     YRTRTGPAPTSQAFRAGGKIDVSEFGPGVAGRISYFITTGRKAEIREIRISGNESVRK
                     ADILSVMTTKPYDFFGSSGSVLPDQVLIDLEKIRGLYRDRGYPEMRFVGVTDGPSMVT
                     REHRDADTELVTYANSQSAFSVEVLDDTEGVYLNIAIDEGKHTRVGFVSIEGNRFLTY
                     REISEMMGLKRGGDYSPAIMNKAMRSLARRYSREGFLGARLKLSCEGFNPDVSRSECD
                     PASVRSDVLNIFLEIDEGRQTMVGEVFVEGAVKTVKSVILKDMPRPGDPYDVEGIARA
                     TRHLNNLGIFESVRVDAIGVDEKPPRDRAALVVRVREAKSKFLDFAIGLEKLDSTRAS
                     DMPAVGSSIITNTLAINDISNTGFGRGLGLNLPDILVTFEMRYSDINFLGRAKRLYLP
                     IKYGLSFTAWDRYASFTPTYLDPNFFARGLMFRVTPYAEYDRATRALDRIQIGSEFAI
                     SKELYRHLFASLSFDTGGVRTRDPQTSKEYDAWRYENKLIPALTYDGLDHPINPTKGF
                     FGQASFSYINDVSEGNYLKWEFSGKAFFSVRRLVTFGIVARIGGSRSFSTSGDLPLEE
                     RYTLGGNRGLRGFSTDGVAQYGADGNPILRVALEPVTDENGKTVVDVDGNPIMGKKYY
                     KPFGGDVMISGSFEMRFPIIQKVQLFGAVFYDFGALSESFSDLMGPSFRHSVGIGIRY
                     MLAGTVPIRLDYGFILDRRYKSFNADTGRFEKMEEIGNFHFGILYTF"
     gene            complement(2352..3278)
                     /locus_tag="PKH54_05845"
     CDS             complement(2352..3278)
                     /locus_tag="PKH54_05845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC99446.1"
                     /translation="MGRIHKFISDAGVAARRKAEELVAEGRVTVNGQTATIGQIIDPS
                     KDRVMVDGNGIRKMPKRIYLMMNKPREVLCTAADARGRRTVFDLLPELPAKVHSVGRL
                     DFMSEGLLLFTNDGDLTRKLTHPSGGLERVYEVKLQGPVRDWVLGKLVSGVQLEDGFA
                     KAVRVSRLRMAQTNEWLSVTLAEGRYREVRRMMEAVGYNVLKLRRVRFGPVELGDLKI
                     GGFRYLTDEEVMALQTGRATPLPKPGTVIRKHVSEEKKKSLGWAVSSKEPRQPRGRQK
                     TTSAVARAPRTPRTPGNAGARRAPRASKVQKV"
     gene            3360..3866
                     /locus_tag="PKH54_05850"
     CDS             3360..3866
                     /locus_tag="PKH54_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HOC99447.1"
                     /translation="MKFKYEHDFQCERKRLISAMFAPGVIESLKDGMKTLTDASTLEW
                     RQEGDKIFRRVRYLLVPIIDEIAGRKVDPKWMEWIEEAEVDTESGRAMFRNVPTTPSI
                     AAIMENTGTMEFVDMGGGVTRRVVSGELKIKVFLVGMVAEVIIAEKARGLLDDEAAAM
                     RRRLQAEG"
     gene            3866..4177
                     /locus_tag="PKH54_05855"
     CDS             3866..4177
                     /locus_tag="PKH54_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC99448.1"
                     /translation="MVLGKVIGTVWGSKQSEGLVSFRVVEIRRVSLQGLQPGARLMAD
                     IEGPMLTESTLLAVDSLGADVGQLVVVSIGSRPRDIVVGPGVTTKNTVIAIVDQASVD
                     C"
     gene            4188..4511
                     /locus_tag="PKH54_05860"
     CDS             4188..4511
                     /locus_tag="PKH54_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC99449.1"
                     /translation="MILARVVGQVWATRKCRRLDGQKLLLVQPLCWHEPDHQTDHVVA
                     VDPVGAEVGQDVVICMGTPARLALGDTRFPVEASVAAIVDDVEMWEGRLERPVHGSGQ
                     NGGAM"
     gene            4508..4789
                     /locus_tag="PKH54_05865"
     CDS             4508..4789
                     /locus_tag="PKH54_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC99450.1"
                     /translation="MIRGMVIGEVWATRKNPALSGRKLLLVSQLAVHIDGTRHFTGRV
                     IVAMDNLDADVGQEVVVAFGSGARNAIEPGSRAVLVDAAVVQIIDGSST"
     gene            4903..>6327
                     /locus_tag="PKH54_05870"
     CDS             4903..>6327
                     /locus_tag="PKH54_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99451.1"
                     /translation="MTRIHWFPVGFLSASALAVVLISGMAGCGGNGDPGIDAGSDAGP
                     EDTAVDKSQSITFINPDPESRKLINGKFKAEFNYQDALGLSATLSVGFVKQPGFVPIV
                     IEAKKPGNYSVELDTTLVPEGNRSIEVTAVASDQRTVSARGKVLVDNMLPLISFLEPM
                     PESGSNFMGDLVLRVKVQDDGNKVHSVKITAGDFSWDWPPEEGTTSDVVDTRVTTTVD
                     GVETHTDLVIPTLGWEGGNVTVKVVADDGVPGHKTEMTSVVTFVETPGFVGGENLPLP
                     TGSSGTMIEGIRTGPADDGIWGVAVAGLDGISLYRRSGASVLEKVAAITTEGCTKFAV
                     AMMDQDDYDDVVAFCGSAETRRIAVIRQNEDLTFSELVSIPIEFDVINLTTGRLNGDD
                     FRDIAFTTTDDTFSTGIILSTSTALGDLDSWASAGVYSGAISPEYVAIGQFASDNRNA
                     VVVGNPVNPQVTIFPVNSQGIATTG"
BASE COUNT         1311 a         1626 c         1947 g         1443 t
ORIGIN      
        1 gaggcactgt tggagaagct gtcctttgac gatgccggaa tctttgacat ttcggaagct
       61 tcccgcggct gggccgagat caagacctgg tacgaggatc gcgggtacct gtttgccgat
      121 gtcgtgatgg attatcgcac ccggactggg ccggctccga cgtcgcaggc ctttcgggcc
      181 ggcgggaaaa tcgatgtttc cgaattcggt ccaggtgtgg ccggtcggat ttcgtatttc
      241 atcactacgg gacgcaaggc cgaaataagg gaaataagga tttccgggaa cgagtccgtc
      301 aggaaagccg acattctttc ggtaatgacc acaaagccat acgacttttt cgggagttcc
      361 ggctcggtcc ttccggatca ggttcttatc gatctggaaa agatcagggg actgtatcgc
      421 gacaggggtt acccggaaat gcgttttgtc ggggtaaccg acggaccttc gatggttacc
      481 agggaacaca gggatgcaga cacggaactg gtgacgtacg ccaacagcca gagcgcgttt
      541 tctgttgaag tgcttgacga taccgagggc gtttatctga acatcgcaat agacgaaggc
      601 aagcacacgc gggttggatt tgtttcgatc gaaggcaacc ggttccttac ataccgggaa
      661 atatcggaaa tgatgggttt gaagcgtggt ggcgactact ctccggccat catgaacaag
      721 gccatgagat ccctggcccg tcgttattcc cgggaaggtt tccttggagc ccggttgaag
      781 ttgtcgtgtg aaggcttcaa tccggacgtt tcaaggtcag aatgtgatcc tgcgtcggta
      841 agatccgatg ttctgaacat cttccttgaa atcgatgaag gccggcagac catggttggc
      901 gaggttttcg tcgaaggcgc cgtcaagacg gtcaagtccg tgatattgaa ggatatgccg
      961 cgtccgggag atccgtacga cgttgaaggg atagcaaggg ccaccaggca tctgaacaac
     1021 cttgggattt tcgaatcagt ccgtgtcgat gcgatagggg tcgatgagaa gccgccaagg
     1081 gaccgggccg cgcttgtggt gcgtgtgcgc gaggcgaagt cgaaattcct ggatttcgcc
     1141 atcggccttg agaagcttga ttcaacaagg gcttcggaca tgccggccgt tggttcgtcc
     1201 atcatcacga atacgcttgc gattaacgac atatccaaca ccgggttcgg aaggggtctt
     1261 ggtctgaacc ttcctgatat cctggtcact ttcgagatgc gttattccga cattaacttc
     1321 ctgggaaggg ccaaacgtct ttatctgccg atcaagtacg gactgtcgtt caccgcatgg
     1381 gacaggtatg cgtcgttcac gccgacatac ctggatccca acttcttcgc ccgagggctg
     1441 atgttccgtg tgacgccgta tgccgaatac gacagggcca ccagggcgct ggacagaatt
     1501 cagattggaa gcgagttcgc gatcagcaag gagctttacc ggcacctgtt cgcgtcgctt
     1561 tctttcgata cagggggcgt tcgcaccagg gatccgcaga catcgaagga gtacgatgcg
     1621 tggaggtacg aaaacaagct gataccggcc ttgacctatg acgggctgga tcatccgatt
     1681 aacccgacga agggtttctt cgggcaggcc agtttttcgt atatcaacga cgtatcggaa
     1741 ggcaactacc tcaagtggga gttttcagga aaggcgttct tcagcgtcag gaggcttgtg
     1801 acgttcggga tagtggcccg gattggcggt tccaggtcgt tcagcacttc cggagatttg
     1861 ccgctcgagg agagatacac gctgggcggc aatcgaggtc ttcgcggatt ttcgaccgac
     1921 ggcgtggccc agtacggcgc cgacggtaac ccgatcttaa gggttgctct tgaaccggtc
     1981 accgatgaaa acggcaagac tgttgtcgat gttgacggca atccgatcat ggggaagaag
     2041 tactacaagc catttggggg tgacgtgatg atctcgggtt cattcgaaat gaggttcccg
     2101 atcatccaga aggttcagct ttttggtgcg gtattctatg acttcggcgc actttccgag
     2161 tcattttcgg atcttatggg tcccagcttc aggcattcag ttggaatagg catcaggtac
     2221 atgcttgcgg gaaccgttcc gatccgtctt gactacggat tcatccttga caggcgttac
     2281 aagagcttta acgccgacac cggtcgtttt gagaagatgg aagagatcgg aaatttccat
     2341 ttcggaattc tttacacctt ctgaaccttg ctggctcgtg gggcgcgcct ggcgcctgcg
     2401 tttccaggtg tgcggggcgt tcgtggggct cgtgcgacag cggaggtggt cttctggcgt
     2461 ccccttggct gacgaggttc cttgctggaa accgcccagc caagggactt cttcttttct
     2521 tccgagacat gcttcctgat gaccgtgccc ggtttgggaa gcggtgtcgc ccttccggtt
     2581 tgaagggcca tcacctcttc atctgtcagg taacggaatc caccgatctt caggtctcca
     2641 agttcgaccg gaccgaaccg gacgcgacgc aatttcagca cgttgtatcc aaccgcttcc
     2701 atcatgcgcc ggacttctcg gtaacgacct tccgcgaggg ttaccgacag ccattcgttt
     2761 gtctgtgcca tcctgagcct ggaaacccgg acagccttcg cgaaaccgtc ctcgagctgg
     2821 acgcccgaga ccagcttgcc caggacccag tccctgaccg gcccctggag cttgacttca
     2881 tacacgcgtt ccagaccgcc ggacgggtgg gtcagcttgc gggtcaaatc gccgtcattg
     2941 gtgaaaagca gcaggccttc gctcatgaaa tcaagccgtc cgaccgaatg gacttttgcc
     3001 gggagttccg gaagcaggtc gaaaaccgtc cttcgtcccc gggcgtcggc cgcggtgcaa
     3061 aggacttcac gcggcttgtt catcatcaga taaatgcgtt tcggcatctt ccggataccg
     3121 tttccatcga ccatcacccg atccttggaa gggtcgatga tctgtccgat ggttgcggtc
     3181 tggccgttga cagtcacgcg gccttcggcg acaagttctt ccgccttgcg ccgtgccgcg
     3241 accccggcat ccgagatgaa tttgtgtatt ctgcccatac atcgggatcc ttaagggggg
     3301 attgccacgc acccacgacg gaaggtatca tgacgccggt ccgcattcaa ggaagcggca
     3361 tgaagttcaa gtacgaacac gactttcagt gtgaaagaaa acgattgatt tcggccatgt
     3421 ttgccccggg ggtgatcgag tcactgaagg acggtatgaa gaccctgacc gatgcctcga
     3481 cgctcgaatg gcgacaggaa ggcgacaaga tctttcggcg ggtcaggtat ctgcttgttc
     3541 cgatcatcga cgaaattgcc ggccggaagg tcgatccgaa atggatggag tggatcgagg
     3601 aagccgaggt tgataccgag tccggacggg cgatgttcag gaacgttcca acgaccccgt
     3661 caatcgccgc catcatggaa aataccggaa ccatggaatt cgttgacatg ggcggtggcg
     3721 taacccgcag ggttgtaagc ggcgagttga agatcaaggt ctttcttgtg ggaatggttg
     3781 ccgaggtgat aattgccgaa aaggcacgcg ggctgctcga tgacgaggcc gcggcgatga
     3841 ggcggcggct gcaggcggaa ggctgatggt tcttggcaag gtaattggga cggtgtgggg
     3901 atcgaagcag tcggaagggc tggtgtcctt ccgggtcgtc gagatccggc gcgtttccct
     3961 tcagggactg cagcccgggg ccaggctgat ggccgacatc gaaggaccga tgctgactga
     4021 atcgaccttg ctggcggttg attcgctggg cgcggacgtt ggtcaactgg tggtcgtgtc
     4081 gattggcagc aggccccgtg atatcgtggt tggtcctggg gtgacgacga aaaacaccgt
     4141 gattgccatt gttgatcagg cttctgtcga ttgctgaagg gtgacggatg attcttgcaa
     4201 gggttgttgg acaggtctgg gcaacaagga aatgcaggcg cctggatggg cagaagttgc
     4261 tccttgtgca gccgctctgc tggcacgagc ccgatcatca gacggatcat gtcgtcgcgg
     4321 ttgatccggt cggcgccgag gttggccagg acgtggtgat ttgtatggga actccggcca
     4381 gacttgccct tggtgacacc aggttcccgg tggaggcatc ggtggccgca atcgtcgatg
     4441 acgttgagat gtgggaaggc cgtcttgaaa ggccggttca tggttccggg cagaacgggg
     4501 gtgccatgtg attcgcggca tggtgattgg cgaagtctgg gcgacccgca agaaccccgc
     4561 cctgtcgggt cgcaagctgc tgctggtttc ccagcttgct gttcacatcg atggaactcg
     4621 ccatttcact ggtcgcgtca ttgttgccat ggacaatctg gacgccgatg tcggccagga
     4681 ggtcgtggtc gcattcggaa gcggggccag gaacgcgatt gagcctggtt cgcgagccgt
     4741 gcttgttgac gcggctgttg tccagataat cgacggaagc tcgacctgac ggggtcgggg
     4801 catttttgat catcacacct acttaatcaa atgtgttgac ggttttcttg acgcgtacgt
     4861 gcgcattcgc aacaatagat cggggtattc ccggggtttg tcatgaccag aattcattgg
     4921 tttcctgttg gttttctttc ggcttcggcc cttgctgtcg tgctgatatc cggcatggcc
     4981 ggctgtggcg gaaacggtga tcccggcatt gatgccggaa gcgatgccgg gcctgaagac
     5041 acggccgttg acaagtccca gtcaatcacg ttcatcaatc cggatccaga atcgcggaag
     5101 ctgataaacg ggaagttcaa ggccgaattc aattatcagg acgcgctggg gctttccgca
     5161 acgctgtcgg tcgggttcgt gaagcagccc ggattcgttc cgatcgttat cgaggcgaag
     5221 aagccgggga actactcggt tgaactcgac acgaccctgg ttcctgaagg caacagatcc
     5281 atagaggtta ccgcggtagc gtccgatcag cgcaccgtgt cggccagggg caaggtcctt
     5341 gtcgataaca tgcttccgct tatatcgttc cttgagccta tgccggagtc gggttcgaat
     5401 ttcatgggcg acctggttct cagggtcaag gtccaggacg atggaaacaa ggttcacagc
     5461 gtcaagataa ctgccggcga cttttcctgg gactggccgc ctgaagaagg aaccacgtcc
     5521 gacgttgtcg atacaagggt caccacgacc gttgacggcg tcgagaccca cacggatctg
     5581 gtcatcccga cccttggctg ggaaggtggc aacgtgactg tgaaggtggt tgccgatgat
     5641 ggtgtcccgg ggcacaagac cgaaatgact tccgtcgtca cattcgtgga aacgccgggg
     5701 tttgtcgggg gtgaaaatct tccccttcca accggttctt ccggaaccat gattgagggc
     5761 atcaggaccg gccctgccga tgacgggatt tggggcgtgg ccgtggccgg ccttgatgga
     5821 atatccctat acaggcgatc cggggcatca gttctggaga aggtcgccgc catcaccacc
     5881 gaaggttgca ccaagttcgc ggtggcaatg atggatcagg acgattatga cgacgtcgtg
     5941 gcgttctgcg gttcagcgga aacaagaaga atcgcggtaa taaggcagaa cgaagacctg
     6001 acattttcgg agctggtgtc aatccccatt gagttcgacg tgatcaatct tacgacaggt
     6061 cggcttaacg gcgatgactt ccgtgacatc gcgtttacca caaccgatga cactttttcg
     6121 actggtatca tcctttcgac ttcgacggct cttggcgacc ttgattcctg ggcttcggcc
     6181 ggtgtctatt ccggtgcaat cagccctgaa tacgtggcaa tcggccagtt tgcaagcgac
     6241 aatagaaacg ccgtcgtcgt cgggaatccg gtcaatccgc aggtaacgat attccccgtc
     6301 aacagtcagg ggattgcgac aacaggg
//
LOCUS       DAOPPA010000146         6325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13491_length_6325_cov_4.349601, whole genome shotgun sequence.
ACCESSION   DAOPPA010000146 DAOPPA010000000
VERSION     DAOPPA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13491_length_6325_cov_4.349601"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKH54_05875"
     CDS             <1..323
                     /locus_tag="PKH54_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HOC99452.1"
                     /translation="FIDTHAMVDAFVKNAGLGLGIDYIHDGGQHEYIPDFVIRLKAES
                     ISERYLLLETKGFDPLKEVKAAAARRWCAAVNSDGSYGHWEYRIAESPESVRKIIDEV
                     CMRT"
     gene            395..1756
                     /locus_tag="PKH54_05880"
     CDS             395..1756
                     /locus_tag="PKH54_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOC99453.1"
                     /translation="MKLTSTVKGNSFTFPDVKAVMARANELKSGDELAGVAATSDMER
                     IAAKRVLADLTLNDIRENPAAPYDKDEVTRLIEADLDKEIFAKVKDWTVAQLREHVLS
                     ANTTGEDLLTLGQGLTSECIAAVAKLMTNMDLALGAAKIRVITHANTTLGLRGRFSVR
                     LQPNHTTDNPDGILASTMEGLSWGMGDALIGVNPVTDTWEATSAILNRLYDFTRAFGV
                     PTQICCLAHITTQMEALRRGTPMDVMFQSLAGSEISCNAFGINNAMLAEGTAMIAEKG
                     TAPGPNLMYFETGQGSELSSGGHHGADQVTMEARCYGLARHYKPFMVNTVVGFIGPEY
                     LYNGRQVARAGLEDHFMGKFHGLPMGCDVCYTNHMDADQNDNENLAIILGAAGCNFFM
                     GLPMGDDIMLNYQSTSFHDNASVRELLGLRPTPEFEQWMERMGLMKAGRLTSSAGNMG
                     LFL"
     gene            1768..2652
                     /gene="eutC"
                     /locus_tag="PKH54_05885"
     CDS             1768..2652
                     /gene="eutC"
                     /locus_tag="PKH54_05885"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HOC99454.1"
                     /translation="MDEKQIQDLVTAAVVEALAKQNAPTKFQPGGNRPAEELKLAKRV
                     ADWAGMPMPLPPALQNWLPDGNRAAYLGATPARLGVGRTGVRPRTDTCLTFREDHAAA
                     KDAVSSCLDENILKQFNIVHLHSAAKDRAEFLRRPDLGRKLDAESVEKIKKTAVKGAQ
                     VQIILVDGLSATALNANVPTILPLILDGLNRAGIRSGTVYAVSLGRVVVSDEVARLTG
                     AECSLLLVGERPGLKTAESMGGYVTYMKARNFNEAMRDVTSNIHRGGLRCEEAGPKLV
                     EGCAKALRDRKTGVDANG"
     gene            2676..3323
                     /gene="eutL"
                     /locus_tag="PKH54_05890"
                     /pseudo
     CDS             2676..3323
                     /gene="eutL"
                     /locus_tag="PKH54_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
     gene            3327..3959
                     /locus_tag="PKH54_05895"
     CDS             3327..3959
                     /locus_tag="PKH54_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HOC99455.1"
                     /translation="MTPKQELECLTERFGRERAKLAKKGKKPFRILESGLELDSKPSG
                     FTHLFDNRSLVPKNHPAIRFRGKLDLLQAYVLDAQQACIAEGLGDIAKDLGDILDYLR
                     RLLAAEVVGTPVPELVVGGLKADELQKLSHNTKRFLNVGWIMPDASMGAAVVKLNLLR
                     AYSREVEIAAIDAHGGDTHMNPADRDAMIHGLNRISSAIHALVCKRLNRT"
     gene            4142..5227
                     /locus_tag="PKH54_05900"
     CDS             4142..5227
                     /locus_tag="PKH54_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOC99456.1"
                     /translation="MPALTIRLDGAVDRNTVASTWRDVPAAVRTARASEIILDLQDVT
                     AMDSAGAALCRVIAETATRRGTRLHVSGVSTHARDALKLFRVSILDDIGPRRRPNMFE
                     QTGDGIDRLIEGTIDMLVLVVDTFYLTVTGIWNRMNRARGEYVLDQMVRIGLESFGIV
                     ALISLLVGLTVALQSASVLHDFGADVYLADFVGISMAREMGPLMTAILVAGRCGSSIA
                     AEISTMVITEEVDALKTMGINPIRHLVVPRFAAITLTQPLLSIMSVFMGILGGLVVAM
                     VYLDQSFVTFNAELWSALTVKDIVTGIIKSVSFAWIIVFIGAHRGFKVHGGAEGVGIA
                     TTSSVVQAIFSVIAADAMFSLIFWFDW"
     gene            5233..6036
                     /locus_tag="PKH54_05905"
     CDS             5233..6036
                     /locus_tag="PKH54_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC99457.1"
                     /translation="MSKEDPIIRVENLTVGYTPGNPIITDLSVEFSRGKISTIVGTSG
                     CGKSTLLKSIIGLLRPWEGRTSVAGNDLNYLEGNELTLALSRIGLLFQQGAMLNSITV
                     AQNVAMPLREHTKLPQSVIREIVRLKLDLVGLGHAIDMLPSELSGGMRKRAALARAMV
                     LDPEVLLCDEPSAGLDPQTAASLDELILNLKATFDMSVVVVTHELPSIDMIADDVVML
                     GRGGQLLFDGSLRQAKQCQVPEVVAFFARSVPSQSGRSKPLLSYLEERE"
     gene            6033..>6325
                     /locus_tag="PKH54_05910"
     CDS             6033..>6325
                     /locus_tag="PKH54_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC99458.1"
                     /translation="MITRAQKVRLGIFLIFSLVVLFGTLGTLAGLKLVEKNDEYTVRF
                     SISLSGLERGASVKYNGIWVGRVEDLNINRKNVSETIVTISLKQGTPIKKDTRA"
BASE COUNT         1317 a         1940 c         1919 g         1149 t
ORIGIN      
        1 acttcattga tacacatgcg atggtcgacg catttgtaaa aaacgccgga ctcggtctcg
       61 gaatcgacta tatccacgac gggggtcaac acgaatacat tcctgacttc gtgattagac
      121 ttaaggcgga atcgatttca gagcggtacc tgctgcttga aacgaagggc tttgatcctt
      181 tgaaggaagt caaagcggcg gcggcacgaa gatggtgcgc agcggtgaat tccgacggca
      241 gttatggcca ttgggaatac agaattgcag aatcacccga atccgttagg aaaatcatcg
      301 acgaagtatg catgcggact tgattggcgt gacagggccc ggcgccctga atccaattga
      361 ccggcctccg atgatacgct agcctaatgc cgctatgaag ctgacttcaa cagttaaggg
      421 caattcattc acgtttccgg acgtaaaggc cgtgatggcg cgggccaacg agctgaagtc
      481 cggcgacgaa ctggccggcg tggcggcgac ttccgacatg gaacgcatcg cggccaagcg
      541 cgtcctggcc gacctgacgc tgaacgacat ccgggaaaac cccgcggcgc cgtacgacaa
      601 ggacgaagtc acccgcctta tcgaagccga cctggacaag gaaatctttg ccaaggtcaa
      661 ggattggacg gtggcgcagc ttcgcgagca cgtcctgtcg gccaacacga ccggcgagga
      721 tctgctgacg cttggacagg gcctgaccag cgaatgcatc gcggccgtcg ccaagctgat
      781 gaccaacatg gatctggcgc tcggcgccgc gaaaatccgg gtcatcacgc acgcgaacac
      841 cacactgggc ctgcgcgggc gcttctcggt ccgacttcag cccaaccaca ccaccgacaa
      901 cccggacggc atcctggcat cgaccatgga aggcctgtcc tggggcatgg gcgacgcgct
      961 gatcggcgtg aacccggtca cggacacctg ggaagcgacg tcggcgatcc tgaaccgcct
     1021 ttacgacttc acgcgggcgt tcggcgtccc gacccagata tgctgcctgg cccacatcac
     1081 gacccagatg gaggcgctgc gccgcggcac accgatggac gtgatgttcc agagcctggc
     1141 cggcagtgaa atcagttgca acgcctttgg aatcaacaac gcgatgcttg cggaaggcac
     1201 cgcgatgatc gcggaaaagg ggacggcgcc ggggccgaac ctgatgtact tcgagaccgg
     1261 ccagggcagc gaactcagca gcggcgggca tcacggcgcc gaccaggtga cgatggaggc
     1321 ccgctgttac gggctggccc gccactacaa accgttcatg gtcaacacgg tcgtcggctt
     1381 catcggtccc gaatacctgt acaacggacg ccaggtcgcg cgcgccggcc ttgaagacca
     1441 cttcatgggc aagttccacg gcctgccgat gggctgcgac gtgtgctaca caaaccacat
     1501 ggacgcggac cagaacgaca acgagaacct ggcgataatc ctgggcgcgg ccggctgcaa
     1561 cttcttcatg ggcctgccga tgggcgacga catcatgctg aactaccagt ccaccagctt
     1621 ccacgacaac gcgtcggtgc gcgagctgct tggactgcgc ccgaccccgg aattcgagca
     1681 gtggatggaa cggatgggcc tgatgaaggc cgggcggctt acgtcgtcgg ctggaaatat
     1741 gggccttttc ctgtagggcg gtgaccgatg gacgaaaaac agattcagga tctggtcacc
     1801 gccgcagtcg tcgaggcgct tgcaaagcag aacgccccga cgaagttcca gcccggtgga
     1861 aaccgtcctg ccgaagaatt gaagcttgcc aagcgcgtcg cggactgggc cggaatgccg
     1921 atgccactgc cgccggccct gcagaactgg ctgcccgacg gcaaccgcgc cgcttacctt
     1981 ggcgcgaccc ctgcccgcct gggcgtcggc cgcaccggtg tccgcccacg caccgacaca
     2041 tgcttgacgt tccgcgagga tcacgccgca gccaaggacg ccgtttcgtc gtgcctggac
     2101 gaaaacattc tgaagcaatt caacatcgtc cacctgcatt ctgccgcgaa ggaccgcgcc
     2161 gaattcctgc gccgacccga cctgggccgc aaactggacg cggaatccgt tgaaaagata
     2221 aagaaaacag ccgtcaaagg cgcccaggtc cagattatcc tggttgacgg tctttcggcg
     2281 acggccctga acgcgaacgt cccgacaatc ctgccgctga tcctggacgg acttaaccgt
     2341 gccggcatca ggtctggaac cgtgtatgcc gtttcgcttg gccgcgtcgt cgtctcggac
     2401 gaagttgcaa gactgaccgg cgccgaatgc agcctgctgc ttgtcggcga gcgccccggg
     2461 ctgaaaaccg cagaatccat gggcggttac gtgacttaca tgaaggccag gaacttcaac
     2521 gaggccatgc gcgacgtcac atccaacatc caccgcggcg gccttcgctg cgaggaagcc
     2581 ggcccgaagc tggtcgaggg ctgcgcgaag gcgctgcgcg accgcaagac cggcgttgac
     2641 gccaacgggt gacaaacccc agtaacggtt gacaaatcat ggttctggaa ccgataaagc
     2701 cgcgcgtcct tggcctgcgg gtcatcccga acgtcgatcc acagcttgca cgcgacctga
     2761 agttgcagcc gaaccaccgc gcgatcggcc tgctgacctg caccagcgac gacgccctgt
     2821 acaccgcgct ggacgaaggc accaaggccg ccgacgtcga tgtcgtctat ggccgcagtt
     2881 tctatgcagg ttcggcgcac gcaagcggcc cgctttcggg cgaggtcatc ggcatcttcg
     2941 ccgccgccga tgaagaaagc gtcgcatccg ccctgaccgc aaccacccgt tatcttgaca
     3001 caaaggcctg gttctattcg gcaaacgacg aatgcagcct ggcgtttttc ccgcacgtca
     3061 taccgtcggt cgggcggtat ctggccggac tggccggcgt cgagcccggc accccgatgg
     3121 cgtacctgat cgccccgccg atcgaggcga tggtcgccct ggacgcggcc ttgaaggtcg
     3181 ccgacgttga aatgaagatt ttcattccgc cgccgtctga aacgaacttc gccgggggct
     3241 tcctggtcgg cgacatatat gcagtccagg ccgcggcggc ggcgttccag gaaaaagtcc
     3301 tggaaatggc ggcacttccc cgcgtgatga cgccaaaaca ggaacttgaa tgcctgaccg
     3361 agcgcttcgg ccgcgaacgg gcgaaactgg cgaaaaaggg aaagaagccc ttccgcatcc
     3421 tggaaagcgg ccttgaactg gattccaagc cgagcgggtt cacgcacctg ttcgacaacc
     3481 gcagcctggt gccgaagaac cacccggcca tccgtttccg cggcaaactg gatctgctgc
     3541 aggcatacgt cctggacgcc cagcaggcgt gtatcgcgga aggccttggc gacatcgcga
     3601 aggaccttgg ggacatcctg gactacctgc gccggctgct tgcggccgag gtcgtcggca
     3661 ccccggttcc cgaactggtg gtgggcggac tgaaggctga cgagcttcag aagctgtcgc
     3721 ataacacaaa gagattcttg aatgtcggct ggattatgcc tgatgcatcg atgggcgccg
     3781 ccgtcgtcaa gctgaacctt ctgcgcgcct attccaggga ggttgagatc gccgcaatcg
     3841 acgcccacgg cggcgacacc cacatgaacc cggcggaccg cgacgcgatg attcacggcc
     3901 tgaaccgcat cagttccgcc atccacgccc tggtctgcaa gcgcctgaac cgcacctgaa
     3961 ccgcatcagt tcggccatcc acgccctggt ctgcaagcgc ctgaaccgca cctgaaccac
     4021 acatgaaccg cgcctgaacc tgtcgctttc ttgaccataa taatgatccg ggctgacaat
     4081 tcatcgcagg gatcggtttg tcaacaggtt gcccggaaaa atggccgcag aaagtccaga
     4141 aatgcccgcc ctgaccatca ggcttgacgg agcggttgac cggaacaccg tcgcaagcac
     4201 gtggcgcgac gtgccggcgg ccgtccggac ggcaagggca tccgaaataa ttctggatct
     4261 tcaagacgtt accgcgatgg acagcgccgg cgcggcactg tgccgggtga ttgccgagac
     4321 tgcgaccagg cggggcaccc ggttgcatgt cagtggcgtc agcacgcatg cccgcgacgc
     4381 cctgaagctg ttccgcgtct cgatccttga cgacatcggg cccagacggc gccccaacat
     4441 gttcgagcag accggcgacg gcatcgacag gctcatcgag ggcaccatcg acatgctggt
     4501 tctggtcgtc gataccttct acctgaccgt caccggcatc tggaaccgca tgaaccgggc
     4561 ccgtggcgaa tacgtcctgg atcagatggt ccgcatcggc ctggaatcct tcggaatcgt
     4621 tgccctgatt tcactgcttg tcgggcttac ggtcgccctt caatcggcat cggtactgca
     4681 cgacttcggc gctgacgtct atcttgccga ttttgtcggc atttcgatgg ctcgcgagat
     4741 gggccccctt atgacggcca tcctcgtggc gggccggtgc ggcagttcca tcgcggccga
     4801 aatctcgacg atggtcatta ccgaggaagt ggatgcgctg aagacgatgg gcatcaatcc
     4861 gatacgtcac ctggtggtgc cccgctttgc cgcgattacg ctgacgcagc cgcttctgtc
     4921 gattatgtcc gtcttcatgg gcattcttgg cggcctggtg gtcgcgatgg tttatcttga
     4981 ccagagtttc gtgaccttca acgcagagtt gtggtccgcg ctgacggtaa aggatatcgt
     5041 caccggcata atcaagagcg tttcgtttgc ctggattatc gtcttcatcg gggcccaccg
     5101 cggattcaag gttcacggcg gcgccgaagg ggtcggcatc gcgacgacat cgtccgtggt
     5161 tcaggcaatc ttcagcgtta tcgcggctga tgcgatgttc agcctgattt tctggtttga
     5221 ctggtaggtg ccgtgagcaa ggaagaccca ataatcaggg ttgaaaacct gaccgtcgga
     5281 tacacgccgg gaaatccgat catcacggat ctgtcggtgg agttctcgcg gggcaagatc
     5341 tcaacaatcg tcggaacttc cggatgcggc aagagcaccc tgctgaagtc gatcatcggg
     5401 ctgctgcgcc catgggaagg ccggacatcc gtcgccggga acgacctgaa ttacctggaa
     5461 ggaaacgaat tgacactggc gctgtcgaga atcggcctgc tcttccagca gggcgccatg
     5521 ctgaattcga ttacggtggc ccagaacgtc gcaatgccgc tgcgcgaaca cacgaaactg
     5581 ccgcagtccg taatccgcga gatcgtgcgg ctgaagcttg acctggtcgg ccttggacac
     5641 gcaatcgaca tgctgccgtc cgaactgtcg ggtggcatgc gcaagcgtgc tgcccttgcc
     5701 cgcgcgatgg tgctggatcc ggaagtgctg ctgtgcgacg agccaagcgc cgggcttgac
     5761 ccgcagaccg cggccagcct ggatgaatta atcctgaacc tgaaggccac cttcgacatg
     5821 tcggtggtgg tcgttaccca tgagctgccc agcatcgata tgattgcaga cgacgttgtg
     5881 atgctgggcc ggggtggaca gcttctgttc gacggttcgc ttcgccaggc gaagcagtgc
     5941 caggtgcccg aagtcgtggc ctttttcgcc agaagcgttc catcccaatc cggccgcagc
     6001 aagccgctgc tgtcatacct ggaggaaagg gaatgattac ccgcgcccag aaagtcagac
     6061 tcgggatatt cctgatcttt tcactggtcg tgctgttcgg cacgctgggc acccttgcgg
     6121 gcctgaagct ggttgaaaag aacgacgaat atactgttcg tttcagcatc tcgctgtccg
     6181 gactcgaaag gggcgcttcg gtcaagtaca acggcatctg ggtcgggcgt gtcgaagatc
     6241 tgaacatcaa ccggaaaaac gtcagcgaga ccatcgtcac gataagcctg aagcagggga
     6301 cgccaatcaa gaaggacacg agggc
//
LOCUS       DAOPPA010000147         6293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13584_length_6293_cov_4.376563, whole genome shotgun sequence.
ACCESSION   DAOPPA010000147 DAOPPA010000000
VERSION     DAOPPA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_6293_cov_4.376563"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..3437
                     /locus_tag="PKH54_05915"
     CDS             198..3437
                     /locus_tag="PKH54_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOC99459.1"
                     /translation="MLAQNAFAEFLKMDSGARAELLEKITGGELYADISREVFARHSA
                     QVGSLKAMQATVDGIQVLTEDEVAALKESVKVVQVAVDDAGKLAQKYEAALQWLELIE
                     KLVNEAGQLSVERTNLEADVDGFRQDAARLELAETAARLAGEWQSLKNARDNLVEQEK
                     RAADLAENLPGLREAEVKADLAVKDAAAAVLSADAAVQQNVPVIRKARELDGDIRGAA
                     NQLGEIRLEVTTKAAAVDELKVALTNIRGQIADSEAILGTVNEQLDKNACDATLESRL
                     GEVRLKLDGLDTDRRKLSVLRDALNEYTEKLNGIGNEIEGLRTRRKELQDSEAQAERD
                     LKGLQKSREDLLFGHDVSWWDLERDRLAKLVADLGRASEAVEVRTKVEEDLKLLADQD
                     ENARKSIDECERGLAENGKIVAILDDQLVLLNKAKNLQVAVMQLSEQREKLLRPGVEC
                     PLCGSLDHPWAAGNVPTDDETDTKMAETMLRLNDLRAASGELDRKKSALQAGIRSNQV
                     AREKAEQALRKSQDILSQNAVSCGFDATASGLSAILGERAENAATAQAGAAVVINAVL
                     AVDTRLADASRTYQDCRERLIQADNDIQKAEVRKDAAAESITKMVTETAQLETRLSVV
                     AAEVAGLVAVWGIGPDRLDHPDAVLQELDRRRQAWSDWQSKKNSLEKDLQSLRAGIAA
                     RQDGLDRDVKDLEKARKRLEDAEATLKNLQARRAQVLSEPDVDAFEAGLAKAARMAMS
                     NRDIAKQSLSSIQSDLKSVLDQIDENAKAIAAGRVGLGAAEAEFSAALSRRGFADEAA
                     FVASRLSDAEIELLAERRAKLDTRKVAIETKTAENARRMAEERDRALTDLDKATVRER
                     LDQAKQDLAAGNRQLGELKNRMDMDNAERERARDQLGKLQEQQVVVRRWEQLNKLIGS
                     SDGKNFRQFAQGITFEHLIHDANKHLKVLSDRYSLQMAPADKHGNASLDLDVVDDWQG
                     GDARTVRNLSGGETFIVSLALALGLSSLASRRIRIDSFFMDEGFGTLDQEALNSAMDA
                     LGELRRDGKLIGIISHVEALKQRIVTRIDVSRQPGGRSTISGPGVERVV"
     gene            3485..>6293
                     /locus_tag="PKH54_05920"
     CDS             3485..>6293
                     /locus_tag="PKH54_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOC99460.1"
                     /translation="MFWLDPMVSGDENLRRTLVLCRTAASANAVKRWIAARGGMVGVE
                     VVTPSGLAMSLKTPSLRPKSGDHLEADPKVPAGHPWESVNARPGLAEVLKGHLADIRI
                     EGLATEDLPAEVWPLLDAGWGGGDDTDALLSLVERIEAGGEAGRLGFAQVFAVGFGLP
                     GTDLLSPPGPNRYFERLLSGLGAIRLAAPVVSPDATPETALAADVVSEAAWVVKNVMD
                     FRDAGGDPEGVLVLFAGADDRDRIASALARSGIPAAVDQAQRLSRHALAILTHSILPW
                     FDDSGSDSVILVGETMRKLFQSPLLGRASYQEAEAAALGTRIAGLKKAASDEDAGQDD
                     ALNAEQDDLALHRRSVTDALKDCHMIRGTLDAWIAALDAVGNNPGAKTSTRRAAILLW
                     QRLEWLKALRGPTIGHLRAYLNQLSLVTFKDRVAMSIQKALGQNAARPATPDVLDDVL
                     GGAVSSGSINRGVTLLDYANYDGRPSRLCLLTGMHSKGVGQAPSPDPFFTDDVAATWG
                     RCGGSAYVSFLMAQAGAAAARAERSLACVVQRAADGRAAVPVSHRLIRLLSHKPADGL
                     RNYGLAVETEETRNHAALVVEDAVVPVAGPVACGDNEADHAALMATVEWIRGGGTYER
                     GVDLAPGAEPPADAGSSVPDTKDPKKLTLAETIATDWGFLPKSIRPYLGDGSAINVAK
                     IARDKVLSTSSGFEPMTHCLFQAYLKTTLGLKKSEDLEEELSAAEVGTAVHAALEEIG
                     TDPRWRPATRADVVESVRFLSESLTAATRRHLEDAVKDLPDATPALAKARLGLANRWG
                     NHLKLYVQARVQSVEELNEDVLLRNLKDFKKSATFEALVADARSKMSVKSHVTAAVSW
                     ISMAIEAVVFGKDPADENLLAHCDNQKVLASVGAWSRDPGTQAAIRQAAATLVRESTL
                     DLTKQGRIVGGAPEWKFGAYDG"
BASE COUNT         1370 a         1640 c         2081 g         1202 t
ORIGIN      
        1 agtcgaacgg ttgtcgatac cgtgcaacct ggacacagcg tcgtgcgcgt ggaaaggcgg
       61 atggaaacct tcagccgtcg agccatgagg tttcagaagt cccttcggga aagccactga
      121 cggacacgaa atcagatacg gctgcatttg ttgcagagat caccggcctg gatttcgggc
      181 gtttcacaaa atcagtgatg ctggcccaga acgcgtttgc cgagtttctg aagatggatt
      241 cgggggcgag ggccgaactg ctggaaaaga taaccggcgg cgaactgtac gcggacatct
      301 ccagagaggt ttttgcgcgc cattccgcac aggtcggatc cctgaaggcc atgcaggcga
      361 ctgtcgatgg catccaggtc ctgacggaag atgaagtcgc cgctttgaaa gaaagcgtca
      421 aggtcgtcca ggtggccgtc gatgatgccg ggaagctggc gcagaaatac gaagcggccc
      481 tgcagtggct tgaattgatt gagaaacttg ttaatgaggc ggggcagttg tccgtcgaac
      541 ggactaacct ggaagcggat gtcgatggat tcaggcagga cgcggcaaga ttggaactgg
      601 ctgaaacggc cgccaggctt gccggggaat ggcagagtct gaaaaacgcc cgcgacaatc
      661 ttgtggaaca ggaaaaacgg gccgccgacc ttgccgaaaa cctgcccggg ttgcgtgaag
      721 ccgaagtgaa ggcggacctg gccgtaaaag atgcggccgc ggctgttttg tcggcagatg
      781 ccgcagtcca gcagaatgtt ccagtgatcc ggaaggcgcg cgagcttgat ggcgatatca
      841 ggggtgccgc aaatcagctt ggtgaaatca ggcttgaagt taccaccaag gctgcggctg
      901 ttgacgaact taaggtcgcc ttgaccaata tccgcgggca gattgcggac agtgaagcaa
      961 tccttgggac tgttaacgag cagttggaca aaaacgcttg cgacgcgacg cttgaatcgc
     1021 ggctgggtga agtccggctg aaactggacg gtttggatac cgatcgcagg aagctgtcgg
     1081 tcctgcgcga cgccctgaat gaatacaccg aaaagctgaa tggaatcggc aatgaaattg
     1141 aaggacttcg gacgcgccgg aaagaacttc aggacagtga agcccaggcc gaacgcgact
     1201 tgaaggggct tcagaaaagc cgcgaagacc tgctttttgg ccatgacgtt tcgtggtggg
     1261 atctggaaag ggatcggctg gcaaaacttg tggccgatct tgggcgcgca tctgaagccg
     1321 tcgaggttcg caccaaggtc gaagaagatc tcaagttgct ggcagaccag gatgaaaacg
     1381 ccaggaagtc catcgatgag tgtgaacgtg gcttggcgga aaatggaaaa atcgtcgcaa
     1441 ttctggacga ccagcttgtc ctgctgaata aggcgaagaa cttgcaggtc gccgtcatgc
     1501 agttgtccga acagcgtgaa aagcttctgc ggcccggcgt cgaatgtccg ttgtgcggtt
     1561 ccctggatca tccgtgggcc gccgggaatg ttccgacaga cgatgagacc gatacaaaaa
     1621 tggccgaaac catgcttcgg cttaatgatt tgcgtgcggc aagcggggaa ctggaccgga
     1681 agaagtccgc attgcaggcc gggatccggt cgaatcaggt tgcccgcgaa aaggcagaac
     1741 aggctcttcg gaagtcgcag gacattcttt cgcagaacgc ggtttcctgc gggttcgatg
     1801 ccaccgcgtc gggactgtcc gcaatcctgg gggaaagggc agaaaatgcc gccacggcac
     1861 aggccggggc cgctgtggtc atcaatgccg tactggcagt cgatacaaga ctggctgatg
     1921 catcgcgtac ctatcaggat tgcagggaaa ggcttatcca ggccgataat gacattcaga
     1981 aggccgaagt cagaaaggat gctgccgccg aatccatcac gaagatggtg accgaaacgg
     2041 cccaacttga aaccaggctc agcgtcgttg ccgcagaagt cgcgggtctg gtggctgtat
     2101 ggggaatcgg gcctgatcgc cttgatcatc ccgacgcggt tctgcaggaa cttgaccggc
     2161 gcaggcaggc gtggtccgac tggcaaagca agaagaacag tctggaaaag gatcttcaat
     2221 ctttgcgggc cggcattgcg gcgcggcaag atggcctcga cagggacgtc aaagaccttg
     2281 aaaaagccag gaagcgcctt gaagacgcag aagccaccct gaaaaacctt caagcccggc
     2341 gcgcgcaggt tctttctgag ccggatgtcg atgctttcga agccggtctg gccaaagccg
     2401 ccaggatggc gatgtcaaac cgcgacatcg caaaacagag cctgtcctcc attcagtccg
     2461 atctgaaatc cgtcctggat cagattgatg aaaacgctaa ggcgattgcc gcgggcaggg
     2521 tcggacttgg ggctgccgaa gccgaattct ctgcggccct ttcgcgcagg ggatttgccg
     2581 acgaagccgc tttcgttgcg tcgcgcctgt ctgacgccga aatcgaactg ctggccgaaa
     2641 gacgcgcgaa attggacacg cggaaggttg ccattgaaac gaagaccgcc gaaaacgcac
     2701 gccgaatggc agaagaacgc gatcgcgccc tgaccgacct tgacaaggcg actgtgcggg
     2761 aacgtcttga ccaggcgaaa caggaccttg cggcaggcaa ccgtcagctt ggtgaattga
     2821 agaaccggat ggacatggac aatgccgagc gcgagcgtgc ccgagaccag cttggaaagc
     2881 ttcaggaaca gcaggttgtg gttaggcgct gggaacagtt gaacaaactg atcggatcgt
     2941 cggatggcaa aaatttcagg cagttcgccc agggaattac cttcgaacat ctgattcacg
     3001 acgcgaacaa acatctgaaa gtgctttctg accgctacag tctgcagatg gccccagccg
     3061 acaaacatgg caatgcatcg ctggatcttg atgtcgttga cgactggcag ggcggcgatg
     3121 cccgcacggt cagaaatctg tccggtggcg aaacattcat tgtcagtctg gccctggcgc
     3181 ttgggctgtc gtcgcttgca agccggcgga ttcgaatcga ttccttcttc atggatgaag
     3241 ggtttggaac actggatcag gaagcgctga actccgccat ggacgcgctt ggtgaacttc
     3301 gtcgcgacgg caagctgatc gggatcatct cgcacgtcga ggcattgaag cagaggatcg
     3361 ttacaagaat cgatgtttcc cgacagcccg gcggccgcag taccatttcc gggcccgggg
     3421 ttgaaagggt tgtgtgagtt gaagtggccc caaaagggcc aggtgaatga tggcgaggtg
     3481 acttatgttc tggctcgatc cgatggtttc tggcgacgag aacttgcgtc gaaccctggt
     3541 tctgtgcagg acggccgcct cggcaaatgc ggtcaagcgg tggattgcgg caaggggtgg
     3601 aatggtcggg gttgaggttg tcacgccgag cgggctcgcg atgtccctga aaacgcccag
     3661 cctgcggccg aaaagtggcg accatctgga agccgatccg aaagttccgg cagggcatcc
     3721 atgggaatct gtcaacgctc ggccggggct tgcggaagtt ttgaaggggc atcttgccga
     3781 cattcgtatc gagggccttg cgaccgaaga tctgcctgcc gaagtttggc cgttgcttga
     3841 cgctggctgg ggcggtggtg acgacacgga tgcgttgctg tcgcttgttg aacgaatcga
     3901 agcaggcggt gaagcaggac gattgggctt tgcccaggtg tttgccgttg gtttcggtct
     3961 gccggggacg gatctgttgt caccaccagg gccgaaccga tatttcgaac gcctgctttc
     4021 cgggcttggc gcgatcaggt tggccgcgcc cgttgtgtcg ccggatgcga cgcctgaaac
     4081 ggcactggct gcggatgttg tcagcgaggc cgcatgggtc gtgaagaacg tgatggattt
     4141 ccgtgatgcc ggtggcgacc cggaaggcgt gctggtgctt ttcgcagggg ctgatgaccg
     4201 ggatcggatt gcgtcggccc tggcgcgcag cggcattcca gcggctgttg accaggccca
     4261 gcgcctgtcg cgccatgcgc tggccatcct gacacattcg atactgccct ggtttgacga
     4321 ttctggttcc gattcggtga ttctggtggg cgaaacgatg cgaaaactgt tccagagtcc
     4381 gcttctggga cgtgcgtcgt accaggaagc cgaggctgcc gccttgggga cgcgtatagc
     4441 cgggctgaag aaggctgcgt ccgatgagga tgccgggcaa gacgacgcct tgaatgcaga
     4501 acaggacgat ctggcgttgc accgcagatc ggtgaccgat gccctgaagg attgtcacat
     4561 gatcaggggc acgctggatg cgtggatcgc ggctttggac gcggtcggta acaaccccgg
     4621 cgcgaagacg tcaacccgca gggcggcgat acttctgtgg cagaggcttg aatggctgaa
     4681 ggcgttgcgt gggccgacca tcggtcacct gcgggcgtat ctgaaccagc tgtcgctggt
     4741 gacgttcaag gatcgggttg ccatgtcaat tcagaaggcg cttggtcaga acgctgcccg
     4801 cccggccacc cccgacgtgc tggacgatgt gctgggcggt gcggtttcca gcggcagcat
     4861 caatcgtggc gtgaccctgc ttgattacgc gaattatgac gggcgtccgt cccggttgtg
     4921 tctgcttacc gggatgcatt cgaagggagt cgggcaggcg ccgtcgcccg atccgttttt
     4981 caccgatgat gtggccgcga cgtgggggcg ctgcgggggc tcggcgtacg tttcattcct
     5041 gatggcgcag gcaggggcgg ctgccgcacg ggcggaacgg tcgctggcct gtgtcgtgca
     5101 gagggcggcc gacgggcgtg cggcggttcc ggtgtcccat cgtttgataa ggctcctgtc
     5161 ccacaaaccg gcggacggtc ttcgcaatta tggcctggcc gttgaaacgg aagaaacccg
     5221 gaaccacgct gccctggtcg ttgaagacgc ggtcgtgccg gttgccgggc ctgtggcctg
     5281 cggcgacaac gaagctgacc acgcggcgtt gatggcgacg gtcgaatgga tccggggcgg
     5341 cgggacgtac gaacggggtg ttgacctggc ccccggcgcg gagccgccag ccgatgctgg
     5401 gtcgtcagtc ccggatacaa aggatccgaa gaaactgacc ctggccgaaa ccatcgcgac
     5461 ggactggggg ttcctgccga agtcaatcag accctacctc ggggacggat ccgcaattaa
     5521 tgttgcgaag attgcgcggg acaaggtgct gtccacatcg tcgggatttg agccgatgac
     5581 acactgcctg tttcaggcat acctgaagac gacgttggga ttgaagaaat cggaggacct
     5641 ggaggaagaa ctcagcgcgg ccgaagtcgg taccgccgtg cacgcggccc ttgaagagat
     5701 cggaaccgat ccaaggtggc ggccggccac cagagcagac gtcgtggaat ctgtcaggtt
     5761 tttgtccgaa tccctgacgg ccgcgaccag acgccacctg gaagacgctg tcaaagatct
     5821 tcccgatgca acaccggcat tggcaaaagc caggttgggt cttgccaaca gatgggggaa
     5881 ccacctgaag ctgtacgtcc aggcccgcgt acaatccgtg gaggaactga acgaggatgt
     5941 actgttgcgc aacctcaaag acttcaagaa atctgcgaca tttgaagcgc tggtggctga
     6001 tgccaggtcg aagatgagcg tcaagagtca tgtgacggct gcggtatcct ggataagcat
     6061 ggcgatcgaa gcggttgtct ttggtaagga tcctgctgat gaaaacctgt tggcccactg
     6121 cgacaaccag aaggtactgg cttccgtggg cgcatggtca agggacccgg gcacgcaggc
     6181 cgcaattcgg caggctgccg ccaccctggt cagggaatca actctggatt tgacgaaaca
     6241 gggccgaatt gtcggtggtg cgccagaatg gaaattcggg gcttacgacg gcg
//
LOCUS       DAOPPA010000148         6287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13603_length_6287_cov_3.947689, whole genome shotgun sequence.
ACCESSION   DAOPPA010000148 DAOPPA010000000
VERSION     DAOPPA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13603_length_6287_cov_3.947689"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKH54_05925"
     CDS             <1..1209
                     /locus_tag="PKH54_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99461.1"
                     /translation="PEPRVSVTDRIGGAALEIDMVPGDGYEPFAARPEDDRSTPAVGI
                     EFSVSPAAVKPIGATRIKAPARHTEELERELEMYRSAGGGSPAIKVLLAVLLVAAAGG
                     LGYLGYLILSEEAGPAVKAVIVEKDPGQQHEAEVASQVPDVAQAPQAAAVVDAVPVGR
                     LDTVPEVKSDVDVAVAAADVPVQPDTRVEMPAPAAPPAVQPVPPPVVAPAVSPAPTVV
                     QEARPAPAVEPRKPAPSPKVEPAAIVPSPQPRPKPEPVREPPAPKPEPVKEQPAPKKA
                     PSVGIQVAPDDYMGHVKAGNAALRRDPEQALVHYEVAESLKPGSTMILAKMGDASKAL
                     NRADTAREYYQRALRTNPNYNPAIVGLARIAREKGQTEEARKYYQRYLDVNASAPAAA
                     EARQFLGSVQ"
     gene            1236..2336
                     /locus_tag="PKH54_05930"
     CDS             1236..2336
                     /locus_tag="PKH54_05930"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOC99462.1"
                     /translation="MDRIISSDRPGRPSGHNSTQEPHPILFDCADAASVVAAALGGLS
                     AVIVVADSTVVRLFPGFGDVLRSGLDMPVLVSEFEPGERAKTMDTVVGCCRAMAAGRF
                     DRQCAVVGFGGGVATDLAGMISALWMRGVKLIQVPTSLLAMADAAIGGKAAVNIPEGR
                     NLVGAVKMPDLAVIAPSFLATLPLPERISGLVEIVKTGFVGDPDLVSEFDSMPAEVDI
                     DWLAGVARRAATTKMDIVSVDPGDSGIRLLLNFGHTVGHAIESVSRYSIGHGIAVAAG
                     MVAESAIGRSMGLVDEIEHRRLVEILNRVGVPPRPAVQPADLLQWMLRDKKNREGGIR
                     IALPANDHRIPSMKSFTTVAVSERQFLECWDD"
     gene            2329..5097
                     /locus_tag="PKH54_05935"
     CDS             2329..5097
                     /locus_tag="PKH54_05935"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOC99463.1"
                     /translation="MTERESGFITCLTGSFGSAAEMESFVQSEPGFLDSANQFIEVRL
                     DLLNLIDDSVRDFVTKHREHILVCCRSRQDGGRWSRSEPERLELLAGFAALGPAFVDV
                     ELSSWMRGGGQSINRACRGGCRVMVSVHEFGSSGFFESLAARLNSTGALAVKLAGVTR
                     DAGDLMALSAAGRMLSAPVRCVVGMGAAGPVTRMRPGDFNSTFTFASAAPGLETAEGQ
                     PDLKTLQAWRFRESAALQPVALIGGWQVAGSPGPNVLNRLFAKDNLGFQYIALPCGDW
                     KQAVGAIRHFGISRFAVTMPFKMQPCQEWPDAVVDRWVELTGVTNTVILDGERAVLKN
                     TDAAGAAHLFDRLGGIAGRSLLVLGGGATARSVAAAATESGATVTVAARTAVAVQKGW
                     PASIPVARFTGWERRGEGLHDVVVNTTPLGGDGRATPLDAAGLRAVLAAVDVVIPATG
                     QTAFVSEARRHKLSFAAGLDFWCAQAPFQLDAFTGRDYQADSVVSAAAEAGHRVEGLM
                     ALSRDRLPGADLDPVCLSVPGSKSITQRYLMLAALSGQPCRIVHPSSATDCTELQAAL
                     GMLGVRFGRTSDSIDVYPAAFPSDPGKVISTGQGGTTFRFMAAMSLVCGFDLRLRPEG
                     TMTGRPMEAMWDALAAMGVMVSHADGVYTFSRIESQSEAGRVDGLPVAGVSVDGRVSS
                     QFASALLLAGQALPGGLEVRITGGSVSRPYIDMTIQSLQRFGATVVGSGDVILVQPGS
                     PGRGRRFDVEYDASLAAFWDAASKVFGRSIEIVNRPAAGATMQGDAAFPDLAEVLSRE
                     EDVEIDLSGTPDLLPPLVILALFRKHRARFGGIAHVRAKESDRLHVLAAGLRKIGAVI
                     DEFDDALVIHPSSLRGPAVLDPRGDHRMAMVFGILSLRVPGLKILDCECVNKSYPAFW
                     QDLDKVRN"
     gene            5094..5618
                     /locus_tag="PKH54_05940"
     CDS             5094..5618
                     /locus_tag="PKH54_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOC99464.1"
                     /translation="MKKIETIAIVGMRCAGKSTVGALLAQKLDATFVDTDRLVEDHLG
                     VSIEDLFQQGREAEFRAAEEDICIQALKSGGVVSLGGGAIASERVRRALWCVMTVFMD
                     ASENLIVSRLEKDGRPSLTGQPASMEAAGVNRMRRPWYMDVSDFVIHVDGKTPEQLSN
                     EILRAIKGFEKDDE"
     gene            5635..>6287
                     /gene="aroC"
                     /locus_tag="PKH54_05945"
     CDS             5635..>6287
                     /gene="aroC"
                     /locus_tag="PKH54_05945"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOC99465.1"
                     /translation="MGGSSTGSSFLVMTFGESHGPAVGAVIDGVVPGIPFTTEMVAAE
                     LDRRRPGRGELVSPRREPDTPEILSGVFDGVTTGHPICILIRNHDANPAEYQAFAKVF
                     RPGHGDQSWLARYGIRDWRGGGRQSGRETAGRVAAGVVAKAVLAPRGVRIRAHVVQIA
                     GISAPGWEDGPVDWAAAAASPVCCADAAASLAMQAAVRQAIEQGDSVGGIVEIVATGV
                     "
BASE COUNT         1067 a         1828 c         2137 g         1255 t
ORIGIN      
        1 cccgaaccgc gggtttccgt gaccgaccgg atcggcgggg ccgcgcttga aatcgacatg
       61 gttcccggtg acgggtatga accttttgcc gcgcgaccgg aagatgatcg atcgactccc
      121 gcggtgggga ttgagttcag cgtgtcgccg gcggcggtga agccaatagg ggcgaccagg
      181 atcaaggctc cggcccgtca taccgaggaa ctggaacggg aacttgagat gtaccgctct
      241 gccggtggcg gcagtccggc catcaaggtt cttctggccg ttcttctggt tgccgcggcg
      301 gggggcctgg gatatctggg atacttgata ctgtctgaag aagccgggcc tgccgtcaag
      361 gcggtcatcg tggaaaagga tccgggccag cagcatgagg ccgaagttgc ttcccaggtg
      421 ccggacgtcg cgcaggcgcc ccaggccgcg gcggtcgttg acgccgtgcc ggtgggaagg
      481 ctcgacaccg ttcccgaagt gaagtcggac gtcgatgtgg cggtggccgc ggccgacgtt
      541 ccggttcagc ctgacacacg tgttgaaatg ccggctccgg cagccccgcc cgccgtccag
      601 ccggtcccgc cgccggtcgt ggccccggcg gtctcgcccg cgccgactgt tgttcaggag
      661 gcgcgccctg cgccagcggt cgaacctcgt aagccggctc cgtcaccaaa ggtggaacct
      721 gctgccattg tgccgtcgcc ccaacctcgc ccgaagccgg aaccggtcag ggaaccgccc
      781 gctccgaagc cggaaccggt gaaggagcag ccggccccga agaaggcgcc atcggtcggg
      841 attcaggtcg cccccgacga ctacatgggt catgtgaagg ctggaaacgc cgctcttcgc
      901 cgcgacccgg aacaggcact ggttcattac gaagtcgccg agtcgctgaa gcccggcagc
      961 accatgatac ttgcgaagat gggtgatgcc tccaaggccc tgaacagggc cgataccgct
     1021 cgggagtact accagagggc cttgcggacg aacccgaatt acaatccagc gatcgtcggg
     1081 ctggccagaa tcgccaggga gaagggccag accgaggagg cccgcaagta ttaccagagg
     1141 taccttgacg tgaatgcaag cgccccggca gccgccgagg ccaggcagtt ccttggttcc
     1201 gtgcagtagt aatccggatt tccggagggc attccatgga ccggatcatt tccagcgatc
     1261 ggcccggaag gccatccggc cataattcca cccaggaacc ccacccgata ctgtttgatt
     1321 gcgcggacgc cgcgtcggtc gtggcggcgg ccctgggcgg cctttcagcc gtcattgtcg
     1381 tggcggattc aacggttgtc cggcttttcc ccggctttgg cgacgtattg cgaagcggcc
     1441 tggatatgcc agtgcttgtg agcgaattcg agcctggcga gcgggcgaag acgatggata
     1501 ccgtagtcgg ttgctgccgt gccatggctg ccggccgttt cgatcgccaa tgcgccgtgg
     1561 tggggtttgg cggcggtgtg gcaaccgatc tggccggaat gatttcggcg ctctggatgc
     1621 gtggcgtcaa actgattcaa gtcccgacat cgcttctggc gatggcggat gcggcgatcg
     1681 gtggaaaggc cgccgtcaac atcccggaag gccggaacct ggtcggcgcc gtgaagatgc
     1741 cggatcttgc agtgattgcg ccgtccttcc tggcgaccct gccgttgccg gaacggattt
     1801 cgggtctggt cgagatcgtc aagaccgggt ttgtcgggga tcccgacctt gtcagtgaat
     1861 tcgattcgat gccggccgag gtcgatatcg actggctggc gggcgttgcg aggcgggcag
     1921 ccaccacgaa gatggatatt gtgtcggttg atccgggtga ttcgggcatc aggctgcttc
     1981 tgaacttcgg ccacacggtc ggtcatgcaa tcgagtccgt ttcccggtat tcgattggcc
     2041 acgggatcgc cgtcgccgcc ggaatggtgg cggagtccgc catcgggcgg agcatgggac
     2101 tggttgacga aatcgagcat cgccggctgg tcgaaatcct gaatcgtgtc ggtgtgccgc
     2161 cgcggcccgc tgtccaaccg gcagatctgc ttcaatggat gcttcgcgac aagaagaacc
     2221 gcgaaggcgg gattaggatt gccctcccgg ccaacgacca ccgtatcccg tcgatgaaat
     2281 ccttcacgac tgttgccgtg tccgaacggc agttcctgga atgctgggat gactgaacga
     2341 gaatccggtt tcatcacctg cttgaccggt tccttcggca gcgccgccga gatggaatcg
     2401 ttcgttcaat cggagcctgg ctttctggat tccgccaatc agtttatcga ggtcaggctt
     2461 gatctgctga acctgatcga tgattcggtc agggacttcg taacaaagca tcgggaacat
     2521 atccttgtct gctgcagatc cagacaggac ggcggtcgct ggtcgcgatc cgagccggag
     2581 cggctggaac tgctggccgg gttcgctgca ctcgggccgg ctttcgtcga tgtggaactt
     2641 tcttcctgga tgcgtggcgg cggccagtcg atcaatcgcg catgccgtgg cggctgtcgc
     2701 gtaatggtat cggttcacga gttcgggtcg tcgggcttct ttgagagtct ggcggccaga
     2761 ttgaattcga ccggtgctct ggccgtcaag ttggccgggg tcacgcggga tgccggcgat
     2821 cttatggcac tttcggccgc cggacgcatg ctttcggccc cggtaaggtg cgtggttgga
     2881 atgggcgccg ccggcccggt gacgcgcatg cgcccgggtg atttcaattc gacattcact
     2941 tttgcatcgg ccgcgcccgg ccttgagacg gccgagggcc aacccgatct gaagacgctg
     3001 caggcatggc gcttcaggga atcggcggcg ttgcagccgg tggctctgat aggaggctgg
     3061 caggttgccg gatctcccgg gccgaacgtg cttaacaggc tgtttgcaaa ggataatctt
     3121 ggctttcagt acatcgcctt gccgtgcggc gactggaagc aggctgtcgg cgcgatccgg
     3181 cacttcggta tttccagatt cgccgtgacg atgcccttca agatgcaacc gtgccaggaa
     3241 tggccggatg ccgtggttga ccgctgggtg gaactgacgg gcgtcacgaa cacagtcatc
     3301 ctggacgggg aacgtgccgt actgaagaac acggacgcgg ctggtgcggc ccaccttttt
     3361 gacaggcttg gcggcatcgc gggccgcagc ttgcttgtgc tgggcggtgg tgcgaccgcg
     3421 cgcagcgtgg ccgccgcggc taccgagtcc ggcgccacgg ttacggttgc ggcaagaact
     3481 gcggttgccg ttcagaaggg gtggcccgct tcaattccgg tggcccgttt caccggctgg
     3541 gagcgtcgcg gcgaaggtct tcacgacgtc gtggttaata cgacgccgct tgggggcgac
     3601 ggacgcgcga caccgcttga cgcggctggg cttcgcgcgg tgttggcggc cgtggacgtg
     3661 gtcattccgg cgaccggtca gactgcattc gtttcggaag cgcgtcggca caagctgtct
     3721 ttcgccgccg gcctggattt ctggtgcgcg caggcccctt ttcagttgga cgcgttcact
     3781 ggtcgcgact atcaggccga ttcggtcgta tcggcggcag cggaggcggg gcatcgcgtg
     3841 gaaggcctga tggcgctttc ccgcgatcgt ctgcccggtg cggatctgga tccggtttgc
     3901 ctgagcgttc caggttccaa gagcatcact cagagatacc ttatgctggc cgccctgtct
     3961 ggccagccat gcaggatagt tcacccatca tcggcaacgg actgcactga gctgcaggcc
     4021 gccctgggaa tgctgggggt caggttcgga cgcacatcgg attcaatcga tgtctatccg
     4081 gcggcctttc cttccgatcc tggaaaggtg ataagcacgg ggcagggcgg aacaactttc
     4141 cgcttcatgg cggcgatgtc gcttgtatgc gggttcgacc tgcgccttcg tccggaagga
     4201 accatgacgg gacgtccgat ggaggccatg tgggacgccc tggccgcgat gggtgtgatg
     4261 gtgtcccacg ccgatggtgt ttacacgttt tcccggatcg aatcgcaatc tgaagccggc
     4321 cgggtggatg gccttcccgt cgcgggcgtt tcggttgacg gccgggtttc cagccagttc
     4381 gcgtctgccc ttttgcttgc cggccaggcg ctgccgggcg gcctcgaggt caggattacc
     4441 ggtggaagcg tttccaggcc atacatcgac atgactatcc agtcgctgca gcgtttcggg
     4501 gcaactgtcg tcggatccgg ggacgttatt ctggtgcagc cgggcagccc cggccggggc
     4561 aggcgctttg acgtcgaata tgatgcatcg ctggcagcct tctgggacgc cgcctcaaag
     4621 gtattcggaa ggtcgattga aatcgtcaac aggccggccg ccggcgcaac catgcagggc
     4681 gacgccgctt tccccgatct tgccgaagtc ctttcccgcg aggaagatgt cgaaatcgac
     4741 ctgtcgggga cgcccgatct tctgccgccc cttgtgatac tggcgctttt ccgcaagcac
     4801 cgggccaggt tcggcggaat cgcgcacgtc agggcaaagg aatcggacag gttgcatgta
     4861 ctggccgcgg ggcttcgaaa gattggcgcg gtcattgacg aattcgacga cgcgctggtg
     4921 attcacccat catcccttcg tggtccggcc gtgcttgacc cgcgcgggga tcaccggatg
     4981 gcgatggttt tcggaattct gtcgcttcgc gttcctggat tgaaaattct ggattgcgaa
     5041 tgcgtcaata agtcgtatcc tgcgttctgg caggatctgg acaaggtaag gaattgaaga
     5101 agattgaaac gattgcgatc gtcggcatga gatgcgccgg caagtcaacc gtgggcgccc
     5161 tgcttgccca gaagctggat gcgactttcg tcgataccga caggctggtt gaagaccatc
     5221 ttggagtgtc gatcgaggac cttttccagc agggacgcga ggctgaattc agggccgccg
     5281 aagaggacat ctgcattcag gcgctgaagt ccggcggggt cgtgtcgctc ggcggcgggg
     5341 ccatcgcttc agagcgggtc aggcgggcct tgtggtgcgt gatgaccgtc ttcatggatg
     5401 ccagcgaaaa cctgattgtg tcccgccttg aaaaggacgg acgtccatcg ctgaccggtc
     5461 agccggcatc gatggaagca gccggcgtta atcggatgcg ccgaccgtgg tacatggacg
     5521 tttcggattt tgtcattcat gtcgatggca agactccgga acagttgagt aacgaaatcc
     5581 ttcgcgcgat caagggcttc gagaaggacg acgagtaatc gccacgaggg ccggatgggc
     5641 ggaagttcaa ctggaagcag cttcctggta atgactttcg gcgaatccca cggcccggcc
     5701 gtcggcgccg tcattgacgg ggtcgtgcct ggaattccct ttacaactga aatggtcgcg
     5761 gccgaacttg accggcgccg tcccgggcga ggcgaactgg tctcgccccg tcgcgagccc
     5821 gacaccccgg agatcctttc gggggtgttt gacggtgtaa ccaccggcca ccccatatgc
     5881 atactgattc gcaaccatga tgccaatccc gccgagtacc aggccttcgc gaaggttttc
     5941 cgcccggggc acggcgacca gtcctggctg gctcgttacg ggatcaggga ctggcgcggt
     6001 ggcggtcggc aatcagggcg cgagacggcc gggcgagtgg cggcgggcgt ggtcgcaaag
     6061 gccgtcctgg cacctcgcgg ggttcggatc agggcacatg tcgtgcagat tgccggaatc
     6121 tcggcgcctg gctgggaaga tggcccggtc gattgggcgg cggctgcggc aagtccggtc
     6181 tgctgtgccg acgcggcggc gtcgcttgcg atgcaggccg ccgtaaggca ggccatcgag
     6241 cagggtgatt cggtcggcgg cattgtcgaa atcgtcgcga ccggggt
//
LOCUS       DAOPPA010000149         6276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13642_length_6276_cov_4.208166, whole genome shotgun sequence.
ACCESSION   DAOPPA010000149 DAOPPA010000000
VERSION     DAOPPA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_6276_cov_4.208166"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..853)
                     /locus_tag="PKH54_05950"
     CDS             complement(71..853)
                     /locus_tag="PKH54_05950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC99466.1"
                     /translation="MKLATRIALITLPLLLLPLSAASADKTVRIVVDPGHGGHNMGAV
                     GPYGVHEKYVTLSIADKLVELLSKEKGVVVFVTRKDDVFVSLRDRAGMANALEADMFL
                     SIHCNAAVNTEASGIETFYLGPGSDSAVELAGRENGTEAAASGHAPDQSLYTLLADMQ
                     FNGSQNESAILAAEIQRSLVSTFREARNREVRQAPFTVLETARMPAVVIETGFITHRD
                     EGKKLLMADHQARIARAIHKAVMEFIRQNDSKPAFARQIELE"
     gene            complement(931..2085)
                     /locus_tag="PKH54_05955"
     CDS             complement(931..2085)
                     /locus_tag="PKH54_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99467.1"
                     /translation="MTDIFKTIVRPAVFGATGSLGRCVCRVLAREGISATHLGGYRNH
                     DLLAALLEPSGATSASFMGTASPALAKVMAASGIGRAFDERRALDGCGSAIVVSSGLA
                     GAEPARLAASMGIPLVLGNKEVAVGWGRLLVNQSRRSSSGCALRPIDSEHAAAAVLLA
                     RAGEQGIARLVITGTGGAVREIPPARRIDLSPARVLDHPVWRMGRKITVDSATLINKA
                     FEVIEAAVLFDLPPEKISVRFDRDARIHAAITTADGRTLAFAGPPEMETPVRIACGLK
                     IPETAEVLEGAAGGAAVESLRTPEGPDRRAIELGHAAIEAGGTMPMVLAVADEIAVTA
                     FLEGRMRFGDIVPFVEYSFDKSIRAARRDPFDPADLLATEAETRQSAQIA"
     gene            complement(2107..3474)
                     /locus_tag="PKH54_05960"
     CDS             complement(2107..3474)
                     /locus_tag="PKH54_05960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC99468.1"
                     /translation="MTRLDADLTCGDFMGDRQRYKILDKLDAGGMAEIYRAKAITLDG
                     FEKSVAIKRILPHLCANPKFVNMFLDEARLAMKLNHANIVQVFDVGRAQDTYFIVMEL
                     VEGRNLRRIFQRLSEVGQRFPVPVAVFLVTEALKGLAHAHERRDGDGHSLGIVHRDVS
                     PPNLLISHSGEVKLTDFGLAKAMTQVELTDPGIVKGKFSYLSPEALDGRAVDYRADIF
                     STGIILWELLANRRLFLGKNEIDTAKMVEKAEVPSLTLLNDQVPEELDQMVRKSLARD
                     PRKRYHSAQEMGDDLMQFLFSRGLKVSSYDLSEFLRSIFAAEEVAEEAITQERINALI
                     SEEILSLSMMRFSGRALPVDGANPIRLEELTRLEEGRISSSDLKGIAPDGLEGGRTRT
                     QGIALVDMLEGRETVPMQAVNTPARRGGMPVGAKIAIGIALAGATGVGVWFLWQNFLG
                     SLLGF"
     gene            3630..4754
                     /gene="dnaN"
                     /locus_tag="PKH54_05965"
     CDS             3630..4754
                     /gene="dnaN"
                     /locus_tag="PKH54_05965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOC99469.1"
                     /translation="MEFTAAKKDFASALAKAQGIPEQRNLASVMANILLESVGSDAVR
                     LTAQSYEVTLVTVFPAHVSSPGRMALTARSLTDRARMLPEAPVNLSIMENQWANLSVG
                     QRSSTIPGILPDNMPEVVPPSPTVEVMLPRALLLEMADRVGFAMSVDEGRPNLNGINI
                     SISPTETGATVDAVATDGHRLSWLSRPIETSAPVERVSAILHRKGVADLKRFLDGVEG
                     DVKVGFLRNAVVFTIDTGYLLVRQIELEYPEFRKVIPSGFRFTVDMDREELVSAVKWA
                     QPSISSDNKSPIIRLRFESGKAVVMARDPERGDANTSMDVAFEGEAFEIAYNNRYLLE
                     ALSAVTEQTVTLSIKGEASASLLTSPSDDGLRQVIMPVRM"
     gene            4803..5957
                     /gene="recF"
                     /locus_tag="PKH54_05970"
     CDS             4803..5957
                     /gene="recF"
                     /locus_tag="PKH54_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOC99470.1"
                     /translation="MRVLSIELTDFRNIRSAQVEFGPGVNFIFGPNGQGKTNLVEAVA
                     FLSWLKSFRTSRSADLIRCDESGGGKSALIGAVLEGRSGRHEVRAEIGRGWRKVVLDS
                     APVCSARETMSLLPVSCLSPDDPAILEGGPEGRRLLLDRFAAMIEPSRTPLFSMYASL
                     VRERNALLRGGCQDAAMLDAVEANMAQVGAECVSARLGAMFRIGARLPAALASMAGTD
                     LGVCVGYSSRWLEDRPVAGGLDSGREFMSKAASALSCALKSRRSADAQLGFTSVGPHT
                     DDVDVVLQGLRARGHASRGQKKVLMLAWKAAEAAELAAERGAEPILVLDDALADLDQG
                     RQSGVVEFLLGYRGQSFITSAVAQPGVFCGSNVIPALAGRFGPDAVSGVD"
     gene            5960..>6276
                     /locus_tag="PKH54_05975"
     CDS             5960..>6276
                     /locus_tag="PKH54_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOC99471.1"
                     /translation="MGRFIIEGGNRISGTMTPSGNKNEALPALAACLLTDQEVVLRNM
                     PRLRDVEVMIEVLESLGGEIRWLEHNVLSVRMKEVRTHRLDPELCRRVRASVLFSGPM
                     TAR"
BASE COUNT         1129 a         1909 c         2003 g         1235 t
ORIGIN      
        1 tcccggtgag ttcgctgtca agggacgggc ttgccccgct ggtgaaggtg ctcggggaaa
       61 tgctctgcgg ttactcaagt tcgatttgac gggcaaacgc gggcttcgag tcgttctggc
      121 ggatgaattc catcaccgcc ttgtggatcg cccgggcaat tctggcctgg tggtcggcca
      181 taagcagctt ttttccttca tccctgtggg ttatgaatcc ggtttcgatg acgaccgcag
      241 gcatccgggc cgtttcaagc acggtgaacg gagcctgcct gacctcgcgg ttcctggctt
      301 ccctgaaggt cgaaaccagc gatctctgga tctcggcggc aagtattgcc gactcgttct
      361 ggcttccatt gaactgcatg tcggcaagaa gcgtgtaaag cgactgatcc ggcgcatggc
      421 cgctcgccgc ggcctcggtt ccgttttccc ggcctgcaag ttccacggcc gaatcgcttc
      481 ccggcccgag atagaacgtc tcgatgccgc tggcttccgt gttgacagcg gcattgcagt
      541 ggatcgaaag gaacatgtcg gcttcaaggg cattcgccat cccggcccga tcccggagcg
      601 aaacgaaaac gtcgtccttc cgggtgacga agacgaccac gcccttttcc tttgaaagta
      661 gttcgaccag cttgtcggcg atgctgagcg tgacgtactt ctcgtggacg ccataggggc
      721 cgacggcgcc catgttatga cccccgtgcc ccggatcgac gacgatccgg acggtcttgt
      781 ccgcggaggc cgccgacagg ggcagaagca gcagcggcag cgtaataagc gcgattctgg
      841 tcgccagttt catcggctgg tcacctccgc catggatcgt aaacgtgcag gggcgcgggt
      901 gccaagaaga cgacccgcat gcggtgcgtg tcaggcgatt tgagcggact gccgcgtctc
      961 ggcttcggtc gcgagcaggt cggccggatc gaacggatcc cggcgcgcgg cacggatcga
     1021 tttgtcaaac gagtattcga cgaatggaac tatgtcgccg aagcgcatcc ttccctcaag
     1081 gaaggccgtg acggcgattt cgtcggccac ggcaagcacc atcggcatgg ttcccccggc
     1141 ctcgattgca gcgtggccaa gctctatggc tcgcctgtcg gggccttccg gggttcgcag
     1201 cgactcgacc gcggcgccgc ccgccgcccc ttcaaggact tcggccgttt ctggaatctt
     1261 cagcccgcat gcgatcctca ccggggtctc catctcgggc gggccggcga aggcgagcgt
     1321 ccgaccgtcc gccgtcgtga ttgccgcgtg aatccgggcg tcgcgatcga atctgacgga
     1381 aattttttcc ggcggaaggt cgaagaggac ggcagcctcg atgacctcga aagccttgtt
     1441 gatcagggtc gccgaatcca cggtgatttt tcggcccatc cgccagacag ggtgatcgag
     1501 aacccttgcc ggggagagat cgattctgcg tgccggcggg atctccctta cggcaccgcc
     1561 ggttcccgtg atgacaaggc gtgcgatccc ctgttcccct gccctggcaa gcagcacggc
     1621 agcggccgcg tgttccgagt cgatcgggcg aagagcgcac ccggacgatg aacgtcttga
     1681 ctggtttacc aggagccgcc cccatccgac ggccacttcc ttgtttccca ggacgagcgg
     1741 gatgcccatc gatgcagcca gccgcgcggg ctcggcgccg gccaggccgg acgagaccac
     1801 gatggcgctg ccgcagccgt ccaatgcacg ccgctcgtca aacgcccgcc cgattccgga
     1861 agccgccatc accttcgcca gcgccggcga tgccgttccc atgaaggatg cggatgtcgc
     1921 gccggagggt tccagaaggg ccgccagcag gtcgtggttc cggtatcccc cgaggtgggt
     1981 ggccgagatt ccttcgcggg caaggacgcg gcagacgcat cgtccaaggg aaccggtcgc
     2041 cccgaagacc gctggtctga cgatcgtctt gaaaatgtct gtcaagtgat ggatccgcgt
     2101 aagacttcaa aaaccgagca gcgagcccag gaaattctgc cagaggaacc atacgccgac
     2161 gccggtggcc ccagccaggg caatgccgat tgcaatcttc gccccgaccg gcattccgcc
     2221 acggcgcgcc ggtgtgttga ccgcctgcat cgggacggtc tccctgcctt ccagcatatc
     2281 gacaagggca atcccctgcg tccttgtcct gccgccctca aggccgtccg gagcaatccc
     2341 tttcaggtcg cttgaggaga tgcggccttc ctcgagcctg gtaagctcct cgagcctgat
     2401 ggggttggct ccgtcaaccg gaagcgcgcg acctgagaag cgcatcatgg agagcgaaag
     2461 gatttcttcc gaaatcagcg cgttgatcct ttcctgcgtg atggcctctt ccgccacttc
     2521 ctcggccgcg aagatgctgc gcaggaattc ggaaaggtcg tacgaggaca ccttcagccc
     2581 gcgggagaaa aggaactgca tgagatcgtc gcccatttcc tgggcgctgt ggtaccgctt
     2641 gcgggggtcg cgggccaggg acttgcgaac catctggtcc agttcctcgg gaacctggtc
     2701 gttcagcagg gtcagggaag gaacttcggc cttttccacc atcttggcgg tatcgatttc
     2761 gttctttcca aggaacaggc gcctgttcgc cagcagttcc cacaggataa tgccggtcga
     2821 gaagatatcg gcgcggtaat cgacggcccg tccgtcgagg gcttccggcg acaggtacga
     2881 aaatttgccc ttcacgatcc cggggtctgt cagttcgacc tgcgtcatgg ccttcgcaag
     2941 gccgaagtcg gtcaatttga cttcgccgga gtgggaaatc agcaggttcg gcggcgagac
     3001 gtcgcgatgg acgatgccga ggctgtggcc gtcgccgtcg cgcctttcgt gcgcgtgcgc
     3061 caggcccttc agcgcttccg tgaccaggaa caccgcgacc ggaaccggaa atctttgccc
     3121 gacctcggaa aggcgctgga aaatcctgcg cagatttcgg ccctcgacca gttccatcac
     3181 gatgaagtag gtgtcctggg cccggccgac atcgaagacc tgaacgatgt tggcgtggtt
     3241 cagcttcatc gccagcctgg cttcgtcaag gaacatgttg acgaatttcg ggttggcgca
     3301 caggtgcgga aggatgcgtt tgatcgcgac cgacttttca aagccatcca gtgtgatggc
     3361 ctttgccctg tagatttccg ccattccgcc ggcatcgagc ttgtcgagaa tcttgtatcg
     3421 ctgtcggtcg cccatgaaat ccccgcatgt caggtcggca tcgagtcggg tcaccgggtt
     3481 cagcccgaac ccggcaaaac ccgattaatc taaacctatt cgacaagaaa ttgaagagca
     3541 cgattgattt tgccatccat accgattgac gcgacggccg cttggtggct acgataatcc
     3601 ccggcaattc ttcattgatg gtggctggca tggaatttac cgcagcaaaa aaagacttcg
     3661 cttccgcact tgcaaaggca caaggcatcc cggaacagag gaatctcgcc agcgtcatgg
     3721 cgaacatact tcttgaatcg gttggcagcg atgccgtccg cctgaccgcc cagtcgtacg
     3781 aggtcacgct ggtcaccgtg ttcccggccc atgtttcaag cccggggcgc atggccctta
     3841 ccgcgcgaag ccttaccgac cgggccagga tgctgcccga ggcccccgtc aacctttcca
     3901 tcatggaaaa ccagtgggcc aacctcagtg tcggccagcg ttcatcgacc attcccggaa
     3961 tccttccgga caacatgccg gaagtcgttc cgccgtcgcc gacggttgaa gtgatgcttc
     4021 cgcgcgccct gctgctggag atggcggatc gcgtgggctt cgccatgtcg gtcgatgaag
     4081 gccgtccgaa ccttaacggc atcaacatca gcatcagtcc gaccgagacc ggcgccacgg
     4141 tggatgcggt cgcgaccgac ggccaccgcc tttcgtggct ttctcgtccc atcgagactt
     4201 ccgctccggt cgagcgcgtc agcgccatcc ttcaccgtaa gggcgtcgcc gacctgaaga
     4261 gattcctgga cggcgtcgag ggcgacgtga aggtcggttt cctgcgcaac gcggtcgtgt
     4321 tcacgatcga taccggatac cttctggttc gccagatcga gcttgaatat ccggaattca
     4381 ggaaggttat ccccagtggc ttccgtttca cggtcgacat ggaccgcgag gaactggtca
     4441 gcgccgtcaa gtgggcgcag ccatcgatct cgtccgacaa caagtcgccg atcatcaggc
     4501 ttcgcttcga gtccggaaag gcggtcgtca tggccaggga tcccgaacgc ggcgacgcga
     4561 acacgagcat ggatgtcgcc tttgaaggcg aggcatttga gatcgcgtat aacaaccgtt
     4621 acctgctcga ggccctttcg gcggtcacgg agcagacggt aaccctttcc atcaagggcg
     4681 aggccagcgc ttccctgctg accagtccct cagacgacgg cctccggcag gtcatcatgc
     4741 cggtccgcat gtgacgtggc cccgccggcc gaccagtccg ccggaataag gagcccatcg
     4801 ccttgagagt cctgtccatc gaattgaccg atttcaggaa catcaggtcg gcccaggtcg
     4861 aattcgggcc cggggtgaat ttcatcttcg gcccgaacgg gcagggcaag acgaacctgg
     4921 tggaggccgt ggcgttcctt tcgtggctga aatcgttccg gacatcgcgt tcagccgacc
     4981 tgatccgctg cgatgagagt ggcggcggaa agtccgcgtt aatcggcgcg gttctggaag
     5041 gcaggtcggg gcggcacgaa gtcagggcgg aaatcggccg cggctggcgg aaggtggtac
     5101 tcgattcggc gccggtctgt tcggcgcgcg aaacgatgag cctgctgccg gtgtcgtgtc
     5161 tgagtccgga cgatccggcg atcctggaag gcggtcccga gggccgaagg ctgctgctgg
     5221 acaggtttgc ggcaatgatc gagccgtcaa ggacgccgct gttttccatg tatgcctcgc
     5281 tggtgcgcga gcgtaacgcc ctgctgcgcg ggggctgcca ggatgcggcc atgcttgacg
     5341 cggttgaggc aaacatggcc caggtcggcg cggaatgcgt ttcggcaagg ctcggcgcca
     5401 tgttccgaat cggcgcgcgg ctgccggcgg ctctggcctc gatggcggga acggatctgg
     5461 gggtatgcgt cggatactcg tccaggtggc ttgaagaccg tccggtcgcc ggcggcctgg
     5521 attccggccg ggaattcatg tcgaaggcgg ccagcgcact ttcctgcgcg cttaagtcca
     5581 ggcggtcggc cgatgcgcaa ctcgggttta cttcggtcgg tccgcacacg gacgacgtcg
     5641 atgtcgtgct tcagggcctg cgagcgcgcg gacacgccag tcgcggccag aagaaggtgc
     5701 tgatgcttgc ctggaaggcg gcggaagccg cggaactggc ggccgagcgc ggcgcggaac
     5761 cgatcctggt gcttgacgat gcgctggctg accttgacca gggacgccaa tccggggtag
     5821 ttgagttcct gctcggatac cgtggtcagt cgttcataac gagcgccgtg gcccagccgg
     5881 gtgttttttg cgggtcaaat gtgattcctg cgcttgccgg ccgtttcggt ccggatgccg
     5941 tgtcgggggt tgattgaaga tgggacgttt cattatcgag ggcggtaaca ggatttccgg
     6001 cacgatgacg ccttccggca acaagaacga ggcgcttcct gcgcttgccg catgtctcct
     6061 gaccgatcag gaggtcgttc tgaggaacat gcccaggctc cgggacgtcg aggtgatgat
     6121 tgaggttctt gaaagcctgg gcggcgagat caggtggctt gaacacaacg tgctgtccgt
     6181 ccggatgaag gaagtccgca cgcacaggct ggatccggag ctgtgcaggc gggttcgcgc
     6241 gtcggttctg ttttcggggc cgatgaccgc aaggtg
//
LOCUS       DAOPPA010000150         6256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13691_length_6256_cov_3.600548, whole genome shotgun sequence.
ACCESSION   DAOPPA010000150 DAOPPA010000000
VERSION     DAOPPA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13691_length_6256_cov_3.600548"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="ricT"
                     /locus_tag="PKH54_05980"
     CDS             <1..1094
                     /gene="ricT"
                     /locus_tag="PKH54_05980"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOC99472.1"
                     /translation="GWIRFPKSGKQALAGAGGHQFRSGDLLLVRTERGDIYARALTDS
                     ARKVFLPAEFFEVVDVLSREEATAALKRDEARELEARRVFVELVRKHELDMHLSEVEI
                     THGDARIVFNFTSPGRVDFRSLVRELAGALKSRIELRQIGVRDETRCTGGLGPCGLSL
                     CCATFLREFTTVTIRMAKVQGLVPNPQKVSGLCGRLMCCLAYEHGVYTDMMKDLPRNG
                     TTIKTPKGEGRVKEVLVMRNVIKVALGPGVVEDFTFEQLGISAARGCGCSGGCIDDGV
                     DEAGDEGAPVDERDAGEPSRQTSGTGERRDRQNGDRNRPEQARGDNRGSSQGERRDPG
                     GQAAGSARQHGAPQDRQGRGGRRRGGQGQ"
     gene            1136..1903
                     /locus_tag="PKH54_05985"
     CDS             1136..1903
                     /locus_tag="PKH54_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOC99473.1"
                     /translation="MFIDAHAHIDRLFFKEGLDGVIDRFRAAGVHGVVTVGSGGIDAM
                     QAAIDVARQNRDIVAAIGCHPHEADRFDDALADAMILMAADSSVRAIGETGLDFHYNL
                     SSRDAQIRAFTSQLEIAARLDLPVVIHSREATALCLDIVRDAGRGRVRGQIHCFTGNV
                     DDARGWLDLGFMVSIPGVVTFRNVPDLLEAVSYVPDDALLIETDSPYLAPVPMRGRTN
                     EPAFIQHTAAAVAAARNQSVEHVATVTSRNAELLFKF"
     gene            1972..5121
                     /locus_tag="PKH54_05990"
     CDS             1972..5121
                     /locus_tag="PKH54_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99474.1"
                     /translation="MNRPVLLLVSCVFLLVACGGGGEPDFDVQQPFDEGGFLDQQTGE
                     DQSGTDTRNDVIPPDSGSDGVSEVVQPDSVIIDDLAGDSAGETGGDTIQDVATPCTRD
                     EECADDNICTTDECGEDGVCRHFCVCEAECGTDDDCAAETCKVASCKVVSGSGDCVSA
                     KCEVTDLDCDDQDPCTTDSCSTATGKCRNVRIPGCGGCKSDTECVDTSECTVDFCDIV
                     AGECVFAPIDCADGDDCTADYCVAGFGCVNAVTCACSVNSQCDDNNACTIDSCTPEGE
                     CVYAGVSCDDGIECTQDSCDRLTGCVNAPAGELCESVAECNDDDNCTIDSCSASGCCD
                     HKEIKDCRRCFSDAECDDGNDCTLDGCNTEVYYCVHIASTGTCSDSNPCTTGDRCQDG
                     ACIPGRLTDCDDGQPCTVDYCGLDGACVNQPIPLCSACQGHDDCDDGSLCTVDSCVEG
                     TCRFVRSICDDGNLCTDDFCDASVGCVARNNSDPCDDEDICTVDDVCLDGQCTGGSGL
                     APCDDGNECTQDWCDPVTGCQVSPLEGPCEDGDPCTTDDYCVLGECVAGAGGCNDDDL
                     CTIDKCRAGVGCEYLTVDCNDRNKCTTDSCDPLKGCINAPIVCDDQDACTVDSCVPSS
                     GECRFERIEIDDDDACTVDECDPTTGVIHKVKNCPHDPCFAASCDPRTGMCSQAPLVC
                     DDQNPCTVDSCNSGTSTCVFQEKDCAVSNKCVEGFCNPKNGNCETAPKDCDDGNQCTN
                     DSCDPAVGCLNQARDGACEDGDFCTVGDTCQAGVCKTGNPRVCDSDQCTQGFCDSSYG
                     CRFSPRNEGLPCSDGSICTAGDKCIGGICIGSPTVSCDDGNPCTTDYCDPREGCVSTN
                     NNNSCDDGLDCTKGDVCRAGACTGTPVVCEQDGNQCTDNVCVEGAGCTSVVLAGQPCD
                     DGLGCTEPDTCDNNAVCAGLPKVCVSPNTCLVGECVEGRDCVFAVRDDGSPCSDGNAC
                     TSNDTCSGGTCKSGAAVDCRDGLSCTEDLCDQSTGCYQRLRENCSCREDNECVVKGSK
                     TCCEYRFGDWGRECYYNCD"
     gene            complement(5135..>6256)
                     /locus_tag="PKH54_05995"
     CDS             complement(5135..>6256)
                     /locus_tag="PKH54_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99475.1"
                     /translation="AAAGATMSSTKSSIGHLLGAAGAVEAIFCVLAIRDQVAPPTINL
                     DNPAVTATCLAPACLADYHCRSAGINGFCRESRCDFSRTWIQVGQFGEPGSQSGQFNS
                     PTDVATVPGPDGDGFQFLVADSRTHYVQVFDGSGKFIRMLGGYGTVPGKFLSPRGIAA
                     SEGRVLIADNLNGRIQVMTPAGVFLYSFGKPGMDPGKFDTPRDVAVSPDSNRIYVADT
                     GNHRISVHNRLGVLMFTFGAFGTSPGRFRTPSAITVGRDGRVWVCDTQNNRIQVFSAD
                     GVLDQVIKPDKELALASPGGIAALPGGGAVVADTYNHRLVFFNDYGKPTGTLGSFGSG
                     TGSFFYPSDIDVIGNGADYRLVIADSGNYRVTEWAVKFW"
BASE COUNT         1207 a         1772 c         2030 g         1247 t
ORIGIN      
        1 tcggctggat aaggttcccc aaatccggaa agcaggccct tgcgggggcc ggtggccatc
       61 agttcaggtc gggcgatctc ctgctggtca ggactgaacg cggggacata tacgcaaggg
      121 cccttactga ttcggcaaga aaggtatttc ttccggccga attcttcgag gtcgtcgatg
      181 tactttccag ggaggaagcg accgcggccc tgaagcgcga cgaggcccgg gaactcgagg
      241 caagaagggt tttcgtcgag cttgttcgca agcacgaact ggatatgcat ctttccgagg
      301 tcgagatcac gcatggcgat gcgaggattg tgttcaattt cacgtcgccg ggccgggtgg
      361 acttccgttc gctggttcgg gaacttgcgg gcgcgcttaa gtcaagaatt gaattgcggc
      421 aaatcggcgt ccgcgacgag accaggtgta ccggcggtct tggcccctgc ggcctgtcgc
      481 tgtgctgcgc gacgttcctt cgcgagttca ctaccgtcac gatcaggatg gccaaggtgc
      541 agggactcgt ccccaatccc cagaaggtat ccggtttgtg tggcaggctg atgtgctgcc
      601 tggcttatga acatggcgtc tataccgaca tgatgaagga tcttcccagg aacggaacca
      661 cgatcaagac cccgaagggc gaaggtcgcg tcaaggaagt cctggtgatg cgaaatgtca
      721 tcaaggttgc cctggggccc ggggtagtcg aggatttcac gttcgaacag cttggaatat
      781 cggccgcgcg cggctgcggt tgttccggcg gttgcatcga cgacggcgtg gacgaggccg
      841 gcgatgaggg agccccggtc gatgaacgcg acgccggcga gccttcacgg cagacgtccg
      901 ggacgggtga acggcgggat cgccagaacg gcgacagaaa ccgtccggag caggcgcggg
      961 gtgacaatcg tggttccagt cagggggaac gtcgcgatcc cgggggccag gctgccggtt
     1021 ccgcccgcca gcacggtgcc ccacaggatc gacaggggcg cggcgggcgt cgtcggggcg
     1081 gtcagggcca gtgatattct ccggccagcg catttaaggg gtcttctgtt tttccatgtt
     1141 tattgatgcc catgcccata tagacaggct cttcttcaag gagggccttg atggggttat
     1201 cgacaggttc cgcgcggcgg gggttcatgg cgttgtcacg gtcggaagcg gcgggatcga
     1261 cgcgatgcag gccgcaatcg acgtggcccg gcagaatcgt gacatcgtgg ccgcgatcgg
     1321 ctgtcatccc cacgaggcgg atcgctttga tgacgcgctg gccgatgcga tgatcctgat
     1381 ggcggccgac agcagcgtca gggcgattgg tgaaaccggc ctggacttcc attacaacct
     1441 ttcctccagg gacgcgcaga tccgggcatt cacgagccag cttgagatcg cggcgcgact
     1501 tgaccttccg gtggttatcc actcccgcga agcgaccgca ttgtgtctcg acatagtcag
     1561 ggatgccggt cgcggtcggg tgcgcggcca gattcactgc ttcacgggaa acgtcgatga
     1621 tgcgagggga tggctggatc ttggcttcat ggtctctatt cctggcgtcg tgactttcag
     1681 gaatgttcca gacttgctcg aggccgtcag ttatgtcccg gacgacgccc tcctgatcga
     1741 aacagattcc ccgtacctgg ctccggttcc gatgcgcgga agaacaaacg agccagcctt
     1801 catacagcac accgcggccg ccgtcgcggc ggcaaggaat cagtctgtcg agcacgttgc
     1861 gacggtgact tcccgcaatg ccgaactgct gttcaagttc tgatttcaat tgattttccc
     1921 cgttcgacac cctagcattg tccggtcagg ctcttgattt tgaggtgcag catgaaccgg
     1981 cccgtcctgt tgttggtttc ctgcgttttt ctgcttgttg cgtgcggtgg cgggggtgag
     2041 ccggattttg atgtgcagca gccttttgac gagggcggct ttcttgatca gcagaccggc
     2101 gaggatcagt ccgggacgga tacccggaac gacgtgattc cgcctgattc cggcagcgat
     2161 ggagtctccg aggtcgttca gccggattca gtcatcatcg atgacctggc tggtgattcg
     2221 gcaggcgaaa ccgggggcga cacgattcag gatgtcgcca ccccgtgcac gcgggacgag
     2281 gaatgtgccg acgacaacat ctgcacgacc gacgaatgcg gggaagacgg cgtttgccgg
     2341 catttctgcg tctgcgaggc cgagtgcggg acggatgacg attgcgcggc cgagacgtgc
     2401 aaggtcgcaa gctgcaaggt tgtttccggc tcgggcgact gcgtgtcggc gaagtgtgaa
     2461 gtcacggatc tggattgcga cgatcaggac ccctgcacca ccgactcctg cagcaccgcg
     2521 acaggcaagt gccgaaacgt caggattccg ggttgcggag gctgcaagtc ggacaccgaa
     2581 tgcgtcgata cgtccgagtg caccgtcgat ttctgcgaca tcgtcgccgg tgagtgcgtt
     2641 ttcgcgccga ttgactgtgc cgacggtgac gactgcaccg ccgactactg cgtcgccggc
     2701 ttcgggtgtg tcaacgccgt gacgtgcgcg tgttcggtga attcccagtg cgacgacaac
     2761 aacgcatgca ccattgattc gtgcaccccg gaaggggaat gtgtttatgc gggcgtatcc
     2821 tgcgacgacg gaatcgagtg tacccaggat tcctgcgatc ggctgactgg ctgcgtgaac
     2881 gcgcctgctg gagagctttg cgagtctgtc gccgaatgca atgacgacga caattgcaca
     2941 atcgattcct gttcggcgag cgggtgctgc gatcacaagg agatcaagga ttgcaggcgt
     3001 tgcttctcgg acgccgaatg cgacgatgga aacgactgta cgctggatgg atgcaacaca
     3061 gaagtctatt actgtgtcca catagccagc acgggcacgt gcagcgactc gaacccctgc
     3121 accaccggtg atcgctgtca ggatggtgcg tgcattcccg gacggttgac cgactgtgac
     3181 gatggtcagc cctgtaccgt tgattactgc gggcttgacg gcgcctgtgt gaaccagccg
     3241 attccgttgt gttcagcgtg ccaggggcac gacgactgtg atgacggttc attgtgcacc
     3301 gtcgattcat gcgtcgaggg gacatgtcgg ttcgtccgtt caatctgcga tgacggcaac
     3361 ctgtgtaccg acgatttctg cgatgcctcg gtcggttgcg ttgccaggaa caattcggat
     3421 ccgtgtgacg atgaagatat ctgcaccgtg gatgacgtct gcctggacgg ccagtgtacc
     3481 gggggatccg gccttgcgcc gtgcgacgac ggcaacgagt gcacccagga ctggtgcgac
     3541 cccgtgaccg gctgccaggt gtcgccgctg gaagggcctt gcgaggacgg cgatccctgc
     3601 acaaccgatg actactgcgt gcttggcgag tgcgtggccg gtgccggcgg ctgcaacgat
     3661 gacgaccttt gcacgatcga caagtgccgg gccggcgtgg gatgtgaata cctgacggtt
     3721 gactgcaacg acaggaacaa gtgcacgacc gattcatgcg atcccctgaa gggatgcatc
     3781 aatgcgccga tagtgtgcga cgaccaggat gcctgcacgg ttgattcatg cgttccatca
     3841 tccggcgagt gccggttcga gcgaatcgaa atcgacgatg acgatgcatg cacggtagat
     3901 gaatgcgatc cgaccacagg cgtcattcat aaggtcaaga attgtccgca cgacccatgc
     3961 ttcgccgcat cgtgcgatcc ccgcacgggc atgtgcagcc aggcaccgct tgtatgcgac
     4021 gaccagaatc cctgcactgt cgattcgtgt aattccggaa ccagcacgtg cgttttccag
     4081 gaaaaggact gcgccgtaag caataagtgt gtcgagggtt tctgcaatcc gaagaatggc
     4141 aactgcgaga cggcgcccaa ggattgcgac gacggcaatc agtgcacgaa cgattcgtgc
     4201 gatcccgccg tcggctgcct gaaccaggcc agggatggtg cctgcgagga cggcgatttc
     4261 tgcacggtgg gcgatacctg ccaggcgggg gtgtgcaaga ccggcaatcc aagggtttgc
     4321 gattccgacc agtgcaccca gggtttctgc gattcttcct atggttgcag gttctcgcca
     4381 aggaacgagg gtcttccgtg cagtgacgga agcatctgca cggccggcga caagtgtatc
     4441 ggcggcatct gcatcggctc cccgaccgtg agctgcgacg acgggaatcc atgcacgacc
     4501 gattactgcg atcccaggga aggctgcgtt tcaaccaaca acaacaattc atgcgacgac
     4561 ggcctggatt gcacgaaggg cgatgtctgc agggccggtg cctgcaccgg gacgcctgtc
     4621 gtctgcgaac aggacggcaa tcagtgtacc gacaatgtct gcgtggaagg ggccggttgc
     4681 acgagtgttg ttctcgccgg ccagccttgc gatgacggtc ttggctgcac cgaaccggat
     4741 acctgcgaca acaacgctgt ctgcgccggt cttccgaagg tctgcgtgtc gcccaatacc
     4801 tgcctggtcg gggaatgcgt cgagggccgt gattgcgtgt ttgcagtccg ggatgacggc
     4861 agtccctgca gcgacggcaa cgcgtgcaca tcgaacgaca cgtgctccgg cggcacatgc
     4921 aagagcggcg cggccgtcga ttgccgcgac gggctgtcct gcaccgagga cctgtgtgac
     4981 cagtcaaccg gatgctatca gcgcctgcgc gagaactgca gttgtcgcga ggacaatgaa
     5041 tgcgtggtga agggttcgaa gacctgttgt gagtaccggt ttggtgattg gggccgcgag
     5101 tgttactaca actgcgactg acgttttccc ggcatcacca gaattttacg gcccattcgg
     5161 taacacggta attgccggaa tcggcaatca ccagccggta gtcggcgcca ttcccgatca
     5221 cgtcgatgtc cgaagggtag aagaagctgc cggttccgga accgaacgag ccgagtgttc
     5281 cggtcggctt tccgtaatcg ttgaaaaaga ccagcctgtg gttgtaggtg tcggcaacga
     5341 ccgcgccacc gccggggagg gcagcgatcc cgcccggact ggccagggcc agttccttgt
     5401 caggtttgat tacctgatca agcacgccgt cggcggaaaa gacctggatt ctgttgttct
     5461 gcgtgtcgca tacccagacc cggccgtccc gcccgacggt gatggccgaa ggcgtcctga
     5521 atcgtcccgg acttgttcca aaggcgccga aggtgaacat gaggacgcca agcctgttat
     5581 ggacgctgat cctgtgattt ccggtgtcgg caacatagat gcggtttgaa tccggcgaga
     5641 ccgcgacgtc cctcggggtg tcgaattttc cgggatccat gccaggcttg ccgaacgaat
     5701 acaggaatac gccggccggg gtcatcacct ggatgcggcc gttgaggttg tcggcaatca
     5761 gcacccgacc ctctgatgcg gcaatgccgc ggggagacag gaacttgccg ggaaccgttc
     5821 cgtatccgcc cagcatgcgg atgaattttc ctgaaccgtc aaagacctgc acataatggg
     5881 tcctggagtc tgcaaccagg aactggaagc cgtcgccgtc aggaccggga accgtcgcca
     5941 catcggtcgg cgagttgaac tggccggatt gcgaccccgg ttcgccgaac tggccgacct
     6001 gtatccaggt tcttgagaaa tcgcatcgcg attcacggca gaaaccgttg attcccgccg
     6061 atctgcagtg gtaatcggcc aggcacgccg gtgccaggca tgtcgcggtc accgccgggt
     6121 tgtccagatt gatcgtgggc ggcgccacct gatcacggat cgcaagcacg cagaaaatcg
     6181 cctcgaccgc gcccgcagcc cccagaaggt ggccaatgct ggatttggtc gatgacatgg
     6241 tggcccctgc cgccgc
//
LOCUS       DAOPPA010000151         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13693_length_6255_cov_3.727258, whole genome shotgun sequence.
ACCESSION   DAOPPA010000151 DAOPPA010000000
VERSION     DAOPPA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13693_length_6255_cov_3.727258"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="recO"
                     /locus_tag="PKH54_06000"
     CDS             <1..780
                     /gene="recO"
                     /locus_tag="PKH54_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOC99476.1"
                     /translation="ETGSGASPAFIISRVPFRDNDLILGLMTRGDGPVSVIARSARNS
                     RRRFFGVLDFFIVLDVVRSPGRGGLDVLVGAEPLRFFPGIHESLERLQAGQSILQAVR
                     NVSRDAPFGPGMFDRLVESFGELEKAPSAAAHMILLETVFALSGELGHAPVDGKCPLC
                     GLAKTAFSINGDGIFACRECCRPTGGCVCQVSFGQSPDAGLYERPVFTMAGGDPPGPA
                     DVSMIVEAVVAGITGHHSPGGGPDGDQPDEDKGQHRQGQHW"
     gene            complement(661..2253)
                     /locus_tag="PKH54_06005"
     CDS             complement(661..2253)
                     /locus_tag="PKH54_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99477.1"
                     /translation="MSQKTRAWWVPLALATLATALPAFTGPAAAQSTGPGVSINELMP
                     LPGQSDPVAGQWIELKNLSDTPINVQGLVLMTSSLDGFHTISPTSDMTLEPGALLVMG
                     PATQSDLNGGAPVTYAYGNDIQMNPQADMVFLMKAGRMIDYVTWGSSSIQIEQGFALS
                     REPGSPTTVNWCRSRASFGAGDHGTPGEENTYCDDDLDTFSEDEGDCDDTNSIIRPGG
                     VEACNGLDENCNGLTDDGVTPPDGVCPSKGVCASIVPVCVGAAGFICPVVAGWEPDGE
                     TLCDGLDNDCDGLTDELLRFNGLTLGTACTSPGECGSGAVVCSPDTLRATCSTMPDGT
                     TPKSSEEICDGLDNDCDGFTDEGFRVGEKCSAGLGACARSGTLACDDDGGARCLAEPG
                     NPQPELCGDRIDNNCDGLTDEGFETGVPCRMGTGACAVWSRYVCAPDMLDVTCPAVPL
                     EPASEICDDLIDNDCNGFTDESGCRRRHGGCSATTGDGTMPSPVLPLSMLTFILIGLI
                     AIRAATGGMMTRDPRDNRLDNH"
     gene            2384..4147
                     /locus_tag="PKH54_06010"
     CDS             2384..4147
                     /locus_tag="PKH54_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99478.1"
                     /translation="MALDSGKIITSCAGLFSAALMVLAAGRAFSQVSPVEEQAQPAAE
                     EIKVVPTSDDTDVVAKPFVTFENHGYFRFRLNTLHNFALGMTPPDGDDPCTPPTAASK
                     GASGVLGNVAGTAVSANIRFRWTPSARIGELLTIGTTLDFLDNLVMGETPFTGDAGYP
                     MAFQARTQSWPSTGISGFKDSVRIKAVWADLFLFGVLHVTGGRVPEHFGLGLVRSGGF
                     HLDSDYGDYVDGLFLKAKIGITYIKAGLEIVGQGVSSDSPFGYFVYPADYSTMDDTLR
                     WTFGLDSSPVTPEDRRQQKERLEAGKPLVNWGMYNAITVQDLSSDRILNSNLAEGGLD
                     VDPKGYEWDKYTTVPRGALFWTPSLWARVEYKPRTDLRFRLEAELAMTYGKVDHLQSN
                     LEETETSKDFLSFGGALEFLIDIGLNQVGLLAGAASGGNTLGYFGINDRHTLACPDGN
                     CYNLTNSEVFLTRNIHHFVFNRDYRIDSILFREIIGSVTNAFYFKPSYFRTFLKERDW
                     NLGGGASLVASFACIKEGTPGGRMPLGVEPALRLAASWKETIFLGADAAVLFPLAGMR
                     YPGQDAPSPAASLRVNATVVF"
     gene            complement(4148..>6255)
                     /gene="uvrA"
                     /locus_tag="PKH54_06015"
     CDS             complement(4148..>6255)
                     /gene="uvrA"
                     /locus_tag="PKH54_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC99479.1"
                     /translation="EAGAERIFSEHLACEECGVSFPELSPQLFSFNNPSGACPSCNGL
                     GSSLSIDPAKVIPDPGLSIAEGAIEPWARAIEDGGLTGEILQSLARQHGIDLDRPFRD
                     LSAEHRDIILFGDLDRIPVKWNSRSFNGSLTVRFEGVASMLLRRLRETKSDEMRSYYQ
                     QYLTDSPCEACHGRRLRPEALAVKVNGMTIDQMSMMPVERLIPAMTDRSGPSEAAAVT
                     DELNREILSRLSLLKNLGLGYLDLARSGATLSGGEAQRIRLASQLGSELTGVTYILDE
                     PSIGLHPRDNQKLINTLCRLRDLGNTVIVVEHDRDAIMAADHVIEFGPGAGRHGGEVT
                     FEGTPSAMLESETSLTGAYLSGRKQIPVPASRRPASGFLTLRGASGHNLKEIDVDFPL
                     GTFTVVSGVSGAGKSSLVTHTLSPAMQNRLNGSRLSCLPFAGIEGVELLDKIIVVNQE
                     PIGRTPRSNPATYTKLFDHIRRLFAETREARVYGYQPGRFSFNVKGGRCEKCQGAGSI
                     RVEMHFLPDVFVTCGECGGKRFNEATLRVRFRDLSIAEVLELPVDEAAAVFKNSGPIS
                     RILSTLQEVGLGYITLGQPSTTLSGGEAQRIKLSRELARMATGRTLYIMDEPSTGLHF
                     DDVAKLLKVVDSLVDRGNTVLMIEHNLEILKVADHIIDLGPEGGERGGMVIGCGTPEQ
                     LASNPQSFTGKSLLELLGA"
BASE COUNT         1160 a         1896 c         1875 g         1324 t
ORIGIN      
        1 gaaacagggt caggagcatc cccggctttt atcatttcca gggttccctt cagggataat
       61 gaccttatcc tgggcctgat gaccaggggc gacggtccgg tctcggtcat tgccagatcc
      121 gcccgcaaca gccggcgtcg cttttttggc gtgcttgatt tcttcatcgt cctcgatgtg
      181 gttcgttccc ctggacgggg cgggctcgat gtgctggtcg gggccgaacc gctgcgtttc
      241 tttcccggca tacacgaatc gcttgagcgg cttcaggctg ggcagtccat cctgcaggcc
      301 gtccggaatg tctcccggga tgccccgttc gggcctggaa tgtttgacag gcttgtcgag
      361 tcgttcgggg aactggaaaa ggctccatcc gcggcagccc acatgattct gcttgaaacg
      421 gtgttcgcgc tttccggcga attgggtcat gcgcccgttg acggaaaatg tccgctctgc
      481 gggctcgcga agacggcctt ctccattaac ggggacggga tatttgcctg tcgggagtgc
      541 tgccgtccga cggggggctg cgtctgccag gtttcgtttg gtcaatcgcc ggatgccggg
      601 ttatacgaaa ggccggtatt cacgatggct ggcggggatc ctccgggtcc ggccgacgtg
      661 tcaatgattg tcgaggcggt tgtcgcgggg atcacgggtc atcattcccc cggtggcggc
      721 cctgatggcg atcagcccga tgaggataaa ggtcagcatc gacaggggca gcactggtga
      781 cggcatcgtt ccgtcaccgg tcgtcgcgga gcatccgcca tgtcgtctgc ggcatccgga
      841 ttcatcagtg aatccgttgc agtcgttgtc gataaggtcg tcgcaaatct cggaagcagg
      901 ttccagcgga accgccggac acgttacgtc aagcatgtcg ggtgcgcaca cgtaccggct
      961 ccatacggcg caggcgccgg ttcccattcg gcagggaact ccggtttcga atccttcatc
     1021 tgtcagcccg tcgcagttgt tgtcaatcct gtcgccgcac agttccggct ggggattgcc
     1081 cggttccgcc aggcaccttg ccccgccgtc gtcatcgcaa gcgagagttc ccgacctggc
     1141 gcaggcgccc aggccggcgc tgcatttttc accaacccgg aatccttcgt cggtgaagcc
     1201 gtcgcaatcg ttgtccaggc catcacagat ttcttccgag gacttcggcg tcgtcccgtc
     1261 cggcatcgtg gagcaggtgg cccgcagagt gtcgggggaa cacaccacag cccctgaacc
     1321 gcactctccc ggtgacgtgc aggccgtgcc gagggtaagt ccgttaaatc ggagaagttc
     1381 atcggtaagc ccgtcgcagt cgttgtcgag cccgtcgcac agggtttccc cgtcaggctc
     1441 ccaccccgcc acgaccgggc agatgaaacc ggcggcaccg acgcataccg gaacaatgga
     1501 ggcgcagact ccctttgaag ggcagactcc atcgggcgga gtgactccgt catctgtcaa
     1561 accgttgcag ttctcatcca gaccgttgca tgcctcgacg ccacccggcc tgatgatgga
     1621 gttcgtgtcg tcgcagtcgc cctcgtcctc gctgaacgta tcaagatcgt cgtcacagta
     1681 ggtgttttcc tcgcccggcg tgccatgatc ccccgcaccg aatgacgccc tggatctgca
     1741 ccagttcacg gttgtcggcg aacctggctc gcgggaaaga gcgaagccct gttcaatctg
     1801 aatcgacgaa cttccccacg tcacgtaatc aatcatccgg cctgccttca tcaggaatac
     1861 catgtcggcc tggggattca tctggatgtc gtttccgtat gcgtacgtca ccggagcgcc
     1921 gccgttcaga tctgactggg ttgcggggcc catcacaagc agggcccccg gctcaagagt
     1981 catgtcggaa gtcgggctga tggtgtgaaa gccgtccagg gaacttgtca tcagtaccag
     2041 gccctggacg ttgatggggg tgtcggacag gttcttcagc tcaatccact gtccggccac
     2101 tggatctgac tgcccgggca acggcatcaa ttcattgatg gacacgccgg gcccggttga
     2161 ctgggccgca gccggcccgg tgaaggccgg cagggccgtc gcgagggtcg ccagcgccag
     2221 cggcacccac cacgcccggg ttttttgtga caagtccatg tccgggatcg tacagcatcg
     2281 ggacaggtaa atggtaacgg attcgggagc gccggttcca ttgacagaaa gggccgtttc
     2341 accttaggat gcttcgactg tctccagggg tggcgtgtac ctcatggccc ttgattccgg
     2401 caaaatcatc acatcgtgcg cgggcctgtt ttcggccgcc ctgatggttc ttgcggccgg
     2461 ccgggccttt tcccaggtgt cgcccgtcga ggaacaggcc cagcccgcag ccgaggaaat
     2521 caaggtcgtc cccacatcgg acgatacgga cgtggtcgcg aaaccgttcg tgacattcga
     2581 gaatcacgga tacttcaggt tcaggctgaa tactcttcac aatttcgcgc tcggcatgac
     2641 gcccccggac ggggacgacc cgtgcacgcc gccgacagcc gcctcgaaag gggcttccgg
     2701 ggtgctcggc aacgtggccg gaaccgccgt gtcggcgaac atccggttca ggtggacgcc
     2761 ttcggccaga atcggggaac tgctgaccat cggaaccacg cttgatttcc tggacaacct
     2821 tgtgatgggc gaaacgcctt tcaccggtga tgccgggtac ccgatggcat ttcaggccag
     2881 aacccagtca tggccttcaa ccggcatttc cggcttcaag gattcggtaa ggatcaaggc
     2941 cgtctgggcc gatctttttc tttttggcgt gctccatgtc accggtggac gggtgccgga
     3001 gcatttcggc cttggtcttg tcagaagtgg tggtttccac ctcgactccg actacggcga
     3061 ttacgttgac ggccttttcc tgaaggccaa gatcggaatc acgtatatca aggccggcct
     3121 ggagatcgtc gggcagggtg tctcgtccga ttcgcccttc gggtacttcg tgtatccggc
     3181 ggactactcg acgatggacg ataccctgcg ctggacgttc ggactggatt cctcgccggt
     3241 gaccccggaa gaccgcaggc agcagaagga aaggctggag gcgggaaaac cgctggtcaa
     3301 ctgggggatg tataacgcca tcaccgtaca ggatctctcc tccgaccgga tactgaactc
     3361 gaacctggcc gaaggcggtc tcgatgtcga tcccaagggt tacgaatggg acaagtacac
     3421 caccgttccc cgcggggccc ttttctggac gcccagcctc tgggccaggg ttgagtacaa
     3481 accaaggact gatcttcgtt tcaggctcga ggccgagctt gccatgacat acggcaaggt
     3541 cgatcacctg cagagcaacc ttgaggaaac ggaaacatcc aaggacttcc tgtctttcgg
     3601 cggcgcgctg gaattcctga tagacatcgg cttgaatcag gttggcctgc tcgccggcgc
     3661 agcaagcggc ggaaacacac tcggatattt cggcatcaac gacaggcaca ccttggcgtg
     3721 tcccgacggc aattgctaca acctgacgaa cagtgaagta ttccttacca ggaacattca
     3781 tcacttcgtg ttcaacaggg attacaggat cgattccatc cttttccgcg agatcatcgg
     3841 atcggttacg aacgcttttt acttcaagcc atcatatttc aggactttcc tgaaggaaag
     3901 ggactggaat ctcggcggcg gcgcctcgct ggttgcctcg ttcgcctgca tcaaggaagg
     3961 cactcccggc ggccgcatgc ccctgggggt tgaaccggcc ttaaggctgg ccgcttcctg
     4021 gaaggaaacc attttcctcg gtgccgacgc agcggttctc tttccgcttg ccggcatgag
     4081 atacccggga caggacgccc cttcgcccgc cgcgtcactg cgggtcaacg cgaccgtcgt
     4141 tttctgatca tgccccaagc agttccagca aagactttcc ggtgaaggat tgcgggtttg
     4201 acgccagctg ttccggagtt ccgcatccaa tcaccattcc gcccctttcg ccgccttcag
     4261 gccccagatc tattatgtgg tcggccacct tcaggatttc caggttgtgt tcaatcatca
     4321 gcacggtgtt tccacgatca accaggctgt caacaacctt cagcagcttt gcgacgtcgt
     4381 cgaagtgcag gcccgtgctt ggctcgtcca ttatgtaaag cgtccttccg gtcgccatcc
     4441 gtgcaagttc cctcgacagc ttgatgcgtt gcgcctcgcc cccggacagg gtcgtcgaag
     4501 gctgcccgag cgtgatgtat cccaggccga cttcctgaag cgtcgataga atccgcgaga
     4561 tcggtccgga attcttgaat acggcggcgg cctcatcgac cggaagttcc aggacttccg
     4621 ctatcgacag gtcgcggaag cgaaccctca gggttgcctc gttgaacctt tttccaccgc
     4681 attccccgca cgtcacgaag acgtcgggaa ggaagtgcat ctcgacacgt atcgaaccgg
     4741 cgccctggca tttttcgcac cgtccaccct tgacgttgaa ggagaacctg ccgggctggt
     4801 agccatatac gcgggcttcc ctggtttcgg cgaacaggcg tcggatatgg tcaaacagct
     4861 tcgtgtatgt tgcggggttg gatcttgggg ttcgtccaat cggttcctgg ttgacgacga
     4921 tgatcttgtc caggagttcg actccttcga ttcccgcaaa cggcaggcag gaaagtcgag
     4981 agccgttgag cctgttctgc atggccgggc tcagcgtgtg ggtgaccagc gatgactttc
     5041 ccgcaccgga cacgcccgat acgaccgtga atgttccaag cggaaaatcg acgtcgatct
     5101 ccttcaggtt gtggcctgag gcgccgcgca gagtcaggaa gccacttgcc ggccggcgcg
     5161 acgccggaac cggaatctgt ttcctgcccg aaaggtacgc cccggtaagc gacgtctccg
     5221 attcgagcat ggcggacgga gtgccctcga acgttacctc tccaccgtga cgtcccgctc
     5281 ccggaccgaa ttcgatcaca tggtcggcgg ccatgatggc atcgcggtca tgttccacga
     5341 cgattaccgt gttgcccaga tcgcgcagtc ggcagagcgt gtttataagc ttctggttat
     5401 cccgtggatg aagcccgatc gacggttcgt ccagaatgta ggttacaccc gtcaattcgc
     5461 ttccaagctg gctggcaagc ctgatgcgct gggcctcccc gccggacagg gtggcgcccg
     5521 accttgcaag gtcaagatat cccaggccca ggttcttcag cagcgacaac cgcgaaagga
     5581 tttccctgtt gagttcgtcg gtcacggcgg cggcttcgga tggccccgac ctgtcggtca
     5641 tcgccgggat caggcgttcc accggcatca tcgacatctg gtcgatggtc atcccgttca
     5701 cctttacggc aagggcctcg ggccgaaggc gcctgccatg gcaggcctcg caggggctgt
     5761 cggtcaggta ctgctggtag tacgaacgca tttcatcgga cttggtttcg cgaagccgtc
     5821 gcagaagcat gctggcgacg ccttcgaacc tgacggtcag cgaaccgttg aagcttcgcg
     5881 aattccactt gaccggaatg cggtcgagat ccccgaacaa tatgatgtcg cggtgttcgg
     5941 ccgaaagatc cctgaaaggc ctatccagat cgattccgtg ctgccgggcg aggctctgca
     6001 ggatttcccc ggtcaggccg ccgtcctcga ttgccctggc ccatggttcg attgcgccct
     6061 cggcaattga aagaccgggg tcgggaatga cctttgccgg gtcgatcgac agcgaggaac
     6121 ccagtccgtt gcaggaagga catgcgccgg aagggttgtt gaatgaaaag agctggggcg
     6181 aaagttccgg gaaggaaacg ccgcattcct cgcaggccag gtgttccgaa aaaatccttt
     6241 ccgcgccggc ttccg
//
LOCUS       DAOPPA010000152         6191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13871_length_6191_cov_4.341428, whole genome shotgun sequence.
ACCESSION   DAOPPA010000152 DAOPPA010000000
VERSION     DAOPPA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_6191_cov_4.341428"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1809)
                     /locus_tag="PKH54_06020"
     CDS             complement(106..1809)
                     /locus_tag="PKH54_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOC99480.1"
                     /translation="MKFLGSHLSYMLEKHQSRKNLKAFVKYLAFLTAVITTFSLLFHV
                     IMLGEGREYSWVTGLYWTLTVMSTLGFGDITFTSDLGRLFSIVVLMSGILLLMIMLPF
                     AFIRFFYAPWLDAHIRTQAPRRIPAETTGHVLLTAWETIQRDLAERLQVLGIPYFVVE
                     PDPVRANELNTEGVPVITGDLDSTRTWRHAGIDRARAVFASLTDEANTNIILTVRELS
                     LTVPIFSLAEKSESVDLLELAGATSVIALKQRLGEQLANRVESTTSSVHVVGKFKNLL
                     IGEFPVFGTELAGKTLGEIHLRQKTGASVVGMWERGQFQRVGRDTVVGQWSIPVVVCT
                     SQQLQAINELIRTDSRNNNPVLVIGGGKIGRATAAQLRTNGLRVNLVERDPVEADRAR
                     PFCDNVFVGDAADRDILQEAGVGSAPSVVITTNDDSTNAYLCIYFRKLNPQIRIVSRI
                     THDRNIESIHRAGADFALSYASLGQESIMALLQGRESIFLGADVDFHLLDVPDALAGR
                     TLRETNIGARTGLNVIAMQQGDTMEITGPDTRLVAGSKMIAIGTAHQIGEMKRVLNQP
                     T"
     gene            complement(1949..3952)
                     /locus_tag="PKH54_06025"
     CDS             complement(1949..3952)
                     /locus_tag="PKH54_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC99481.1"
                     /translation="MLDLFDIRKSFGSRTLFSGVNLRVNPRDRLGLVGPNGTGKTTLF
                     RIILNEISPDSGRVEVQKNIRMGYLAQEVHPSHAGTMGLAEYCVLNARGVGPLQKERA
                     ACVAELERLGHDNDNDAAHVALAKRLAHVEDRLLAVDADHMPGMARSILVGLGFAPED
                     VDRPMNTMSGGLLMRVELARLLLDEPDLLLLDEPTNHLDLEGILWFENYLSTYKGAVV
                     MIAHDRQFLNRTVTHVVEVGPHGATDFGGDPSLPVYDRYVIERAKWVELQWKRYYEQQ
                     DRIAEMEDYIARNRARLSSASAAQSRMKALDKMERIQPPSDLKKVHFRFPPPPRIPDL
                     ALSVEDVTRRYGDRTVFSNLNLRLHRGERMCLIGLNGAGKSTLLRLCAGIARPDEGRV
                     AVLDTVSVGYFAQDSYEILNPESTVEKSMMEVADFTTAPFVRHILGAFLFSDDDYDKK
                     VVTLSGGEKARLVLARLLLQPFGILVLDEPTNHLDISSREILESAIMQHSGAIIFTTH
                     DRRFMDNVATTTVELKDGQATRWEGNYSYYLSRTGGLLAQTVDQKREINEVAAQKRAA
                     DRDRKRDEAERRNRLYRMLKPLRDRVESYEKQISTVEADLKVVEEELVSPTLYEDMDR
                     ARQVSQRAKELRDRLDSLFDEWTEAASELASREQSESEAQPGQ"
     gene            4125..4805
                     /gene="rpe"
                     /locus_tag="PKH54_06030"
     CDS             4125..4805
                     /gene="rpe"
                     /locus_tag="PKH54_06030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOC99482.1"
                     /translation="MSSGNPPFSIKVSPSLIAGNQGRLADEVVALEQAGADMIHLDVM
                     DGHFVPNLTMGPGIIADLRPVSSLPFDTHLMLSEPWKYIDAFVKAGSDRISVHVETEG
                     VGDTLRRIRDAGVSPGIVINPPTPVEMVSEYLGLVDFVLVMTVNPGFYGQKLIPEALD
                     KLPVLRKNSDAFGRDIAIQVDGGINLSNIERVARAGADEVVGGAAVLKAADYRQAIET
                     LRRGGIRP"
     gene            4809..4882
                     /locus_tag="PKH54_06035"
     tRNA            4809..4882
                     /locus_tag="PKH54_06035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4843..4845,aa:Pro,seq:cgg)
     gene            5291..5809
                     /gene="hxsD"
                     /locus_tag="PKH54_06040"
     CDS             5291..5809
                     /gene="hxsD"
                     /locus_tag="PKH54_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HOC99483.1"
                     /translation="MPAKKADSRSEFMATVKGDAAVIRFHKSLYSTDALFGTAFVFLD
                     RCYVHCDMPDNDHIVVTLVPRAGSKWSLAELAGEFKNEMVSQALRFKLAKQTEKLRTM
                     IVGRAIGESIPPDAPAQAPSSAMVPDLPPEVAKLLSEEEESLDFLDDPFGIAVPWEEK
                     YAKEEGRNDQQK"
     gene            5897..6112
                     /locus_tag="PKH54_06045"
     CDS             5897..6112
                     /locus_tag="PKH54_06045"
                     /inference="COORDINATES: protein motif:HMM:NF038315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HxsD-like protein"
                     /protein_id="HOC99484.1"
                     /translation="MKTSFHKKIYPAKAVKVAAEAFAEVAEIHVARDGDYTTVSIESG
                     NPDEDVEYVGEFENFVLGETIAMRGLK"
BASE COUNT         1236 a         1849 c         1891 g         1215 t
ORIGIN      
        1 atgaaaattc gcccgctcga acgatactgg gcaccgtgct tgttcgcagc gggcggatgt
       61 aatccgcccg tacaaggaaa ttcggccggc ccatacgggg aaaggtcacg tcggctggtt
      121 gagaacgcgc ttcatttcac cgatctggtg ggccgttccg atggcaatca tcttcgagcc
      181 ggcgaccagt cgggtgtcgg gaccggtgat ttccatcgta tcgccctgct gcatcgcgat
      241 gacgttaagg ccggtgcgcg cgccgatgtt cgtttcgcgc agcgtcctgc cggcaagcgc
      301 gtccgggacg tccagcaggt ggaaatcgac atccgcgccc aggaaaatcg actcacggcc
      361 ctgcagcagc gccatgatcg actcctggcc aagggacgcg taacttaacg cgaaatcggc
      421 gccggcgcgg tggatcgatt cgatgttgcg gtcgtgggtg atgcgcgaaa cgatccttat
      481 ctgcggattc agctttcgga agtaaataca aaggtacgca ttggttgaat cgtcgttggt
      541 ggtaattacg accgagggcg ccgaaccgac ccccgcttcc tgcaggatgt cgcgatcggc
      601 cgcgtcgccg acgaacacgt tgtcgcagaa tggacgggcc ctgtccgcct cgaccggatc
      661 gcgctcgacc aggttgacac gcagtccgtt tgtccgcaac tgggccgcgg tcgcccgacc
      721 gatcttcccg ccgccgatga ccagcaccgg attgttgttc cggctgtcgg tccttatcag
      781 ctcgttgatc gcctgaagct gttgtgacgt gcagacgacg accggtatgc tccattgacc
      841 gacaaccgta tcccggccga cacgctggaa ctggccgcgt tcccacatcc cgacaacgct
      901 ggcgccggtc ttctggcgaa ggtggatctc gccaagcgtc ttgccggcca gttcggttcc
      961 gaaaaccgga aactcgccga tcagcaggtt cttgaatttc ccgacgacat gaaccgaact
     1021 ggtcgttgac tcgacgcggt tggcaagctg ctcgccaagc ctctgcttca gcgcgatgac
     1081 cgaagtcgcc ccggccagtt ccagcagatc gaccgactcg ctcttttccg ccagcgagaa
     1141 tatcggcacc gtcaaagaca gttcgcgaac cgtcaggatg atattcgtgt tcgcttcgtc
     1201 ggtcagggac gcgaacacgg cgcgcgcgcg gtcaatgcca gcatgacgcc aggttctggt
     1261 cgaatccagg tcgcccgtga tgacaggtac cccttccgta ttcaattcgt tcgccctgac
     1321 cggatccggc tcgacgacga agtacggaat cccgagaacc tgcaggcgtt cggccagatc
     1381 acgctgaatg gtttcccaag ccgtcagaag gacgtgcccg gttgtttcgg cgggaatcct
     1441 gcgcggcgcc tgggtacgga tgtgggcgtc aagccagggc gcgtaaaaga accggatgaa
     1501 ggcgaatggc agcatgatca tcagcagaag aatgccggac atcagcacga cgatgctgaa
     1561 aagacggcca aggtcgcttg tgaaggtgat gtcgccaaac ccgagcgtgg acatcacggt
     1621 aagcgtccag taaaggcccg tgacccacga atactccctg ccctcgccca gcatgatcac
     1681 atgaaaaagc agggaaaacg tcgtaattac tgccgtcagg aaggccagat acttgacgaa
     1741 agccttcagg ttcttccggg actggtgctt ttcgagcatg tacgaaagat gactgcccag
     1801 aaacttcatg agttcaccgt cgaaaaattt cagccgccgc ccggcgatgg ttgtccaggc
     1861 caacgtgacg tcaagaatca tatcacgggc gaagaccttg attccgtggg cgcgagtggc
     1921 ctgcgggtat gtatgccggg cggtggggct actggcccgg ctgggcttcc gattcggact
     1981 gttcgcggct ggccagttcc gaggcggctt ccgtccactc gtcaaacagc gagtccagcc
     2041 gatcgcgcag ttcctttgcc cgctggctta cctggcgcgc ccgatccatg tcttcataaa
     2101 gcgtcggcga aaccagttct tcctcgacca ccttcaaatc ggcctcgacc gtcgatatct
     2161 gcttttcgta tgactcgacg cgatcccgaa gcggcttcag catcctgtaa agccggttcc
     2221 tgcgttcggc ctcgtcgcgc ttccgatccc ggtcggccgc cctcttctgg gcagcaacct
     2281 cgttgatttc ccttttctga tcgacggtct gcgccagaag gccgcccgta cgtgaaaggt
     2341 aatagctgta attgccttcc caccgcgtgg cctgcccgtc cttcagttcg acggtcgttg
     2401 tcgccacgtt gtccatgaac cgcctgtcgt gcgtcgtgaa gatgatggca ccggaatgct
     2461 gcatgatggc cgattcaagg atttcacgcg aagagatgtc caggtggttg gtcggttcgt
     2521 caagcacaag gatcccgaac ggttgcagaa gcagccttgc aagaaccaac ctggcctttt
     2581 caccgcccga aagggtaacc accttcttgt cgtagtcgtc gtccgaaaaa agaaacgcgc
     2641 ccaggatgtg ccgcacaaac ggggcggtcg tgaaatccgc cacttccatc atcgactttt
     2701 cgaccgtcga ttccgggttc aggatttcat acgaatcctg ggcaaaatac ccgacgctaa
     2761 cagtgtccag gactgccacg cggccttcgt ccggcctggc gatcccggcg cacaggcgca
     2821 gaagcgtcga tttgccggcg ccgttgagcc cgatcaggca cattctttcg ccacgatgca
     2881 ggcgaaggtt caggttcgaa aacacggtcc ggtcgccgta tctcctggtg acgtcctcga
     2941 cgctcagggc caggtcgggg atgcgtggcg gaggcggaaa gcggaagtgg acctttttca
     3001 ggtcggatgg cggctggatt cgttccatct tgtccagcgc cttcatcctg gactgggcgg
     3061 ccgacgcgga cgacaacctg gcccggttgc gcgcgatgta atcttccatt tccgcgatgc
     3121 ggtcctgctg ttcgtaatac cgcttccact gaagctcgac ccacttcgcg cgttcaatca
     3181 cataacggtc atacaccggc aacgaaggat ccccgccgaa atcggtcgcg ccatgcggcc
     3241 cgacttcgac gacgtgcgtc accgtcctgt taaggaactg ccggtcgtgg gcaatcatga
     3301 cgaccgcgcc cttgtacgtc gaaaggtagt tttcaaacca caggatgcct tcaaggtcca
     3361 ggtggttggt cggttcgtca agcagcagaa gatccggttc atcaagcagc aggcgcgcaa
     3421 gttcgacgcg catcagaagc ccgccggaca tggtgttcat cgggcgatcg acgtcttccg
     3481 gcgcgaaacc cagcccgacc aggatggaac gtgccatccc tggcatatga tcggcatcga
     3541 ccgccagcag gcgatcctcg acatgggcca gccttttcgc aagcgcgacg tgggccgcgt
     3601 cgttgtcgtt gtcatgcccc agccgctcga gttcggccac gcacgcggcg cgttccttct
     3661 gcagcgggcc gacgccgcgt gcgttaagca cgcagtattc ggccaggccc atcgtcccgg
     3721 catgactcgg gtgcacttcc tgggccagat atcccatgcg gatgttcttc tggacctcga
     3781 cgcggccgga atcgggactg atttcgttca agattatccg gaaaagcgtg gttttgccgg
     3841 tgccgttggg gccgaccagc ccaaggcgat ccctggggtt gacccgcagg ttcacaccgg
     3901 aaaacaacgt ccggcttccg aaggattttc ttatgtcgaa caggtcgagc atggcgcaga
     3961 aggttaaccc aacacgcccc gaatgggaac aggaaccgtg ccgtcggttc cgggccgggg
     4021 ttgccgcggg cctgggcaaa tggtatgaat ggcctgctgt cacgggcgcc gcgaacttcg
     4081 ccgcacccgc gcccgttctg attcccgttg cgaggtcacc caggatgtca tccggcaacc
     4141 caccgttttc aatcaaggtt tcaccgtccc ttatcgccgg caaccagggc cggcttgccg
     4201 atgaagtcgt tgcgcttgaa caggccggcg ccgacatgat tcaccttgac gtgatggacg
     4261 gacacttcgt cccgaacctt acgatgggcc cgggaatcat cgccgacctg cggccggttt
     4321 cgtcgctgcc gttcgacacg cacctgatgc tgtccgagcc atggaagtac atcgatgcct
     4381 tcgtgaaagc gggatccgac aggataagcg tccatgtcga gacggaaggg gtcggtgaca
     4441 ccctgcgccg gataagggac gccggggttt cgccgggcat cgtcatcaat ccgccgacgc
     4501 cggtcgaaat ggtgtcggaa tatcttgggc ttgtcgattt cgtcctggtc atgacggtca
     4561 acccgggttt ttacggacag aagctgatac cggaagctct tgacaaactg ccggtgctgc
     4621 gcaagaattc cgacgctttt ggtcgggaca tcgcgatcca ggtcgatggc ggcatcaacc
     4681 tttcgaacat cgaaagggtc gccagggccg gtgcggacga ggtcgtcggc ggggcagcgg
     4741 tcctgaaggc cgccgattac cgccaggcaa tcgagacgct gcgacgcggt ggcatccgac
     4801 cttgacaacg gggcgtagcg cagtctggta gcgcacctgg ttcgggacca gggagtcgct
     4861 ggttcaaatc cagtcgcccc gactaatcaa caataaaaaa tcacaggccc ggtgggcaat
     4921 ttttacgcag acgggcgaat tttcattcgc ccttacggat cggggggcgg atcgcatcag
     4981 cccggggcag gcaaccgacg ggcgcgatgg aaggcatttt gctcgaccgc agcgggcgga
     5041 tgtaatccgc ccgtacgagg aagggagcgc tgtgataatt attgtcatta gtgttaaata
     5101 atgtaatccg cccgtacgag gaagggaggc tggcaactgc atcttgcaga gccgcggcgg
     5161 gcggatgtaa tccgcccgta cgagggccga cgtgccttga caaggctttt tcgcgggcgc
     5221 tatgatgctg cggcatttgt ccggcctcgg gggggccgcc aacacaccga tttttctgga
     5281 ggacagagtg atgcctgcca agaaggccga ttccaggtcc gaattcatgg ccaccgtaaa
     5341 gggtgacgcc gcggtgatac gtttccacaa gtcgctttac tcgacggacg cgcttttcgg
     5401 cacggcgttc gttttccttg accgctgcta tgtccactgc gacatgccgg acaatgatca
     5461 catcgtcgtg acgctggtcc ccagggccgg gtcgaagtgg tcgctggccg aactggccgg
     5521 cgagttcaag aacgagatgg tcagccaggc gctgcggttc aaactggcga agcagaccga
     5581 aaaacttcgc acgatgatcg tcggccgcgc catcggcgaa tcgattccgc cggacgcacc
     5641 ggcccaggcg ccctcctcgg ccatggttcc ggatctgccg cccgaggtcg cgaagctgct
     5701 ttcggaagaa gaagagagcc tggacttcct ggatgatccg ttcgggatcg cggttccgtg
     5761 ggaagaaaag tacgcgaagg aagaaggcag gaacgaccag cagaagtgac cgtcggccac
     5821 ccgccgtccg cgggccgggc gtgacgttcc ggtcacggac ttcccgattg acggcgccgg
     5881 tgttgcgggg gatgacatga agaccagttt tcacaaaaag atttatccgg ccaaggccgt
     5941 gaaggtcgcc gccgaggcgt tcgccgaggt cgccgaaatc cacgtggcca gggacggtga
     6001 ttacacgacg gtatcaatcg agtccggaaa cccggacgag gacgttgagt acgtcggcga
     6061 attcgaaaac ttcgtcctgg gcgaaaccat tgcgatgcga ggacttaaat gagcacgaag
     6121 cccgaaagaa acatgatgcg gtggcgccag gtcggcgaca agttcgtggt caccaacgac
     6181 gcgggcgagt t
//
LOCUS       DAOPPA010000153         6186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13895_length_6186_cov_3.910781, whole genome shotgun sequence.
ACCESSION   DAOPPA010000153 DAOPPA010000000
VERSION     DAOPPA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13895_length_6186_cov_3.910781"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PKH54_06050"
     CDS             <1..1150
                     /locus_tag="PKH54_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99485.1"
                     /translation="GVALLFAVHPAVGEPVSWVSGRKDLLAAMFSVMALASFLGIPAR
                     DRGIRARLPAAGLCLLAALSKPSALLVPVLLLVLDLRRPVRPGDAPLEVRESHWAWRK
                     GAGLSLWAFIIGINAALAVIAHRLETGMGALQGSRGSDLVERVLAGAGWHARIVFWPF
                     DLLPKYLDPAAGPSMPTLAIGGLVVVGMLALVVWTGIRRHPAFVGLALAVAAYIPQSG
                     VVPLSRQYANSYIYLVLAGLALAAGAAVSPLLSRTARPVRMMAGVAAVILVVALGLAA
                     HSQGAVYRDGVKLWSHVWQAYPDSPQVCRNLGNAYIYGDVDQPEKAVEVFQGCIVSLG
                     NRPFFLKNLAVATYRAGHRQQARQLFLELRQTRPGDPVADKYLRLLSE"
     gene            complement(1245..3413)
                     /locus_tag="PKH54_06055"
     CDS             complement(1245..3413)
                     /locus_tag="PKH54_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99486.1"
                     /translation="MQHLKNPLRLAAAAVATVAAAVIVAGCADGSNGNPDIVECIPTC
                     AEGQECGGDGCGGFCGTPDGMCADGLKCDGMSCIANPCPAGFVLVPAWTYRMGSPDNE
                     PGRVTGGGDETLHKVTISKPFCMAETEVTNAEWAAVTATTPSYFKDAGDNVAVQNINW
                     YDAIKFLNMKSVGDELTPCYLTDMESGTHGGGCVTGQIQCNGDFHFGSVSRVPGCTGY
                     RLPTEAEWELAARAGHDTDPDEFEGLQPAQGLQCQSPNPRLDPFAWYCGNAAATYESA
                     YDCRDWSPLETCGPQPVARKDPNAWGLYDMLGNVFEFTQDQYWWHTADPQTDPLLDDE
                     NGYNRVIRGGSWSAAAAFARVASRAAMGPTDRGDVGLRPVIDPPTSALGPMIQARLAA
                     GLDGTDDSARTNRIAPAAGNMAPETSGRSSTDGSGAEAAMPEPAPGAEDQEQEVVFAG
                     PTYRVVSFNLMCSVCGAGYDDWEERLVYFGDMFETIQADLIGIQEIVFDFEVDQLLAE
                     LPGYAAYFYKGDGADFFAYPDATVLYRTDRFELLDQGEYWLSPTPDVPWSMGYSPGQS
                     IPRVVAWVKLRDRTTSQIVYFATTHYDSTHPHQEHSAPQMIRTVEQWAVEAPVIQTGD
                     FNTEPRDPAFNILTNGIDATGFHMDDTFATVGNYWNSDVNTPKNPPYDPINRIDHIFT
                     AGADFSTKFWWVDLRKFGPDTLYPSDHRLIWAEIVMNGPY"
     gene            3808..5124
                     /gene="rmuC"
                     /locus_tag="PKH54_06060"
     CDS             3808..5124
                     /gene="rmuC"
                     /locus_tag="PKH54_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOC99487.1"
                     /translation="MLNILIIIAIVMLAAVLAMLVAVMGKVGRLNAANTDATVRDEMQ
                     RVRQEMETHSRGLRKEVAEQQERANNTLVGTMHKLGEEQRGVLKDITTQNIEALDRMR
                     IAVENKFAQIQESNEKKLDEMRRTVDEKLQTTLEKRLGESFKLVSERLEAVQRGLGEM
                     QTLASDVGGLKRVLTNVKDRGTWGEYQLGAILSEILTPDQYAQNVSPKNNSDRVEYAV
                     RLPGRAEDGGDPVWLPIDAKFPKEDYERLLDAVQAADMDGVKKCGDALARAVRTSAKD
                     IHDKYINPPLSTDFGIMFLPSEGLYSEILRQPGLVGEIQNSYRVIVAGPTTLSSILSS
                     LRVGFQTLAIEKRSHEVWKVLAAVKTEFETFGDFLEKVQKQINAASKTLESAGTRTKK
                     MKSKLSSVQSLSVAEAATILSLPEKGDAMPDDDYEAEAGNAEGELV"
     gene            5296..>6186
                     /locus_tag="PKH54_06065"
     CDS             5296..>6186
                     /locus_tag="PKH54_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99488.1"
                     /translation="MKEILKEIIRDSHEAPIVTGIPRRLNTPAIPGKAGVFMGVRRSG
                     KSTYMNQLMQGLIDGGLPRQNILAVNFFDDRLAELRRQGPAAVIDAYWSRFPEKKNVE
                     TIHCFFDEIQEVADWEPFVDRLLRTENCQVYLTGSSAKMLSSEIATQMRGRSIPWEVF
                     PFSFPEYLDSRGIQAPAVPTSRQRFLIRQAFDEFMLRGGFPEVTGFDRRLRTKVHQEY
                     FGAIMYRDIVERHNVAHPRMLSELTRRLIDNAGSMYSHNSLTGFLKSTGHKATKAIVS
                     DFLEWLQDAYLLFSVKLFDSS"
BASE COUNT         1144 a         1774 c         1950 g         1318 t
ORIGIN      
        1 cggggtcgcg cttcttttcg cggtgcatcc cgctgtcggg gaacctgttt catgggtgtc
       61 cggccgcaag gatctgctgg ccgcgatgtt ctcggtgatg gcgctcgcgt catttcttgg
      121 cattcccgcg cgtgatcgcg ggattcgggc aaggcttccc gccgccgggc tctgcctgct
      181 tgcggccctt tccaagccat cggcgcttct cgtcccggtt cttctgctgg tgcttgacct
      241 tcggcggccg gtccgtccgg gcgacgcgcc cctggaagtg cgggaaagcc actgggcctg
      301 gcgcaagggg gctggtcttt ccctgtgggc gttcatcatc gggattaacg cggcgcttgc
      361 cgtgattgcc cacaggctgg aaaccggaat gggtgccctg cagggttcgc gcggaagcga
      421 cctggtcgaa cgcgtgcttg cgggcgccgg ctggcatgct cggatcgtgt tctggccgtt
      481 cgacctgctg ccgaagtacc tggatccggc ggcagggcct tcgatgccga cacttgccat
      541 cggcggcctg gtcgtggtcg ggatgcttgc ccttgtcgtg tggaccggca tccgccgtca
      601 cccggcattt gtcgggctgg ccctggctgt cgcggcatac atcccgcagt cgggcgtcgt
      661 gccgttgtcg cgccagtatg ccaacagtta catctatctg gtgcttgcgg gcctggcact
      721 ggccgccggg gcggcggtgt cgccgctgct gtcgcggaca gcaaggccgg tcaggatgat
      781 ggccggggtc gcggccgtga ttcttgtcgt ggcgcttggc cttgccgcgc attcccaggg
      841 tgcggtctat cgtgacggag tgaagctgtg gtcgcacgtc tggcaggcgt atcccgacag
      901 cccgcaggtc tgccgcaacc ttggaaatgc gtacatttac ggtgacgtgg atcagccgga
      961 aaaggccgtc gaagtctttc agggttgcat cgtctcgctt ggcaacaggc cgttcttctt
     1021 gaagaatctt gcggtggcaa cctatcgcgc cggccaccgc caacaggcac gtcagctttt
     1081 tctggagttg cgccagacgc gtccgggcga tcccgttgcc gacaagtact taagacttct
     1141 gtcggaatag cttccggaca aggatttcca caaaatcaaa ttctgtttgc ctgtcaggct
     1201 ggtgcgtcgg gccggatatg ttggtaagac tccggaaggc cgtttcagta cggaccgttc
     1261 atcacgatct cggcccagat caggcggtga tccgaagggt acagggtgtc gggcccgaac
     1321 ttgcgcaggt caacccacca gaacttcgtt gaaaaatcag ctcccgccgt gaagatgtgg
     1381 tcaatccggt tgatcgggtc gtatggcggg ttcttcggtg tgttgacgtc gctgttccag
     1441 tagtttccaa cggttgcgaa cgtgtcatcc atgtggaatc cggttgcgtc gattccgttt
     1501 gtaaggatgt tgaacgccgg gtcgcgcggt tcggtattga agtcgccggt ctggatgacg
     1561 ggggcttcga ctgcccactg ctcgaccgtc cggatcatct ggggcgcgct gtgttcctgg
     1621 tgcggatgcg tcgagtcgta gtgcgtggtc gcgaaataga cgatctggct ggttgtcctg
     1681 tcccgcaact tgacccaggc cacgaccctg ggaatcgact ggccgggcga gtaacccatc
     1741 gaccacggaa cgtcgggcgt cggcgaaagc cagtattcgc cctggtcaag cagttcgaac
     1801 cggtccgtgc ggtaaaggac ggtcgcgtcc ggataggcga agaagtcggc accgtcgccc
     1861 ttgtagaagt acgcggcgta acctggaagt tcggccagca actgatcgac ctcgaaatcg
     1921 aagacgattt cctggatgcc gatcaggtcg gcctggattg tctcgaacat gtcgccgaaa
     1981 tagaccaggc gttcttccca atcgtcgtag ccggcgccgc acacgctgca catcaggttg
     2041 aacgacacca cgcggtacgt cggacccgcg aagacgacct cctgttcctg gtcttctgcc
     2101 ccgggggcag gttccggcat ggctgcttcg gccccgctgc cgtcagtcga gcttcttccg
     2161 gatgtttctg gcgccatgtt tcctgcggct ggcgcgatcc ggttcgttcg cgcgctgtca
     2221 tcggtgccgt caaggcctgc cgcaaggcgg gcctgtatca tcggccccag tgcagaggtc
     2281 ggcgggtcaa tgacgggccg caggccgacg tcgccccgat cggtggggcc catggccgcg
     2341 cgggaagcca ccctggcaaa ggccgcggct gccgaccacg agccgccacg gatcacccgg
     2401 ttgtacccgt tttcgtcgtc aagcagcggg tccgtctggg gatcggccgt gtgccaccag
     2461 tattgatcct gggtgaattc gaacacgttg cccagcatgt cgtacaggcc ccaggcgttc
     2521 ggatccttcc gcgcgacggg ctgcggaccg catgtttcaa gggggctcca gtcacggcag
     2581 tcgtatgctg attcatacgt tgcggcggcg tttccgcagt accacgcgaa cggatccagg
     2641 cgggggttcg gggattggca ctgcagtccc tgggcaggct gaaggccttc gaattcgtcg
     2701 ggatcggtgt cgtggccggc acgcgcggca agctcccact cggcttcggt cggcaggcgg
     2761 tatccggtgc atccaggcac acgggaaacc gacccgaagt ggaagtcgcc gttgcactgg
     2821 atctggccgg taacgcaccc tccgccatgc gtgcccgatt ccatgtccgt caaatagcag
     2881 ggcgtcagtt cgtcgcccac ggatttcatg ttcaggaact tgatggcgtc gtaccagttg
     2941 atgttctgga ctgccacatt atcgcccgcg tccttgaaat atgatggggt cgtcgccgtg
     3001 actgccgccc attcggcgtt ggtcacttcg gtttcagcca tgcagaaagg ctttgaaatc
     3061 gtgaccttgt gaagggtctc gtcgccgccg ccggtgacac ggcccggttc gttgtccggc
     3121 gagcccatcc tgtacgtcca cgccgggacc aggacgaagc cggccgggca gggattcgcg
     3181 atgcacgaca tgccgtcgca cttcaggccg tcggcgcaca tgccgtcggg cgtgccgcag
     3241 aatccgccgc atccatcgcc cccgcattcc tggccttcgg cgcaggtggg gatgcattca
     3301 acgatgtcgg gattgccgtt gctgccgtcg gcacagccgg cgacgataac ggccgccgcg
     3361 accgttgcca cggccgctgc cgccagtcgc aaggggttct tcaggtgctg cattttgtat
     3421 tgcctcgatg ggcgattgtt gcaggtggca gtttacaacc tggggcgtgg aaattgaatc
     3481 actggcggag atcttgccgg acatcgtcgc gctcggtgaa gatatcccgt tcctgtggag
     3541 gttctcggtc gcgatgcaac atgaagtcct gcgggactct gtcgggactg gcaaaaaagt
     3601 cgtcccacgg agatgcgact tgttcagttt gagtcatttt gacctccaga aacctggtac
     3661 gccgcaagag taacatattt ttcaggcagg aatcttgccg ccagacaggt tatctttcgg
     3721 ttgaaacaga atccggtgta cggcggaaga cggaaggggc aatatctggg ttacggcgcc
     3781 ccggtggcgc gcttgagggg gttatccatg cttaacatat tgattatcat cgcgattgtg
     3841 atgcttgcgg ccgtgctggc gatgctggtt gctgtcatgg gtaaggtcgg caggctgaac
     3901 gcggcgaaca ccgatgctac cgtccgcgac gaaatgcagc gtgtccgtca ggagatggaa
     3961 acccacagcc gcggcctgcg caaggaagtc gctgaacagc aggaacgagc caacaatacg
     4021 ctggtcggca ccatgcacaa actgggcgag gagcagcgcg gcgtcctgaa ggacatcacc
     4081 acgcagaata tcgaggccct tgaccgcatg cggatcgcgg tcgaaaacaa gttcgcgcag
     4141 atccaggaaa gcaacgaaaa gaagctggac gagatgcgca ggaccgttga tgaaaagttg
     4201 cagaccacgc ttgaaaagcg tctgggcgag tcgttcaagc ttgtttccga aagactcgag
     4261 gctgtccagc gcggccttgg cgagatgcag accctggcgt ccgacgtggg cggtttgaag
     4321 cgcgtcctga ccaacgtcaa ggatcgcggc acctggggcg aataccagct cggcgccatc
     4381 ctgtcagaaa tcctgacccc tgaccagtac gcgcagaacg tgtcgccgaa gaataactcg
     4441 gatcgcgtcg agtacgcggt caggcttccc ggccgcgcgg aagacggtgg cgatccggtc
     4501 tggctgccga ttgacgccaa gttccccaag gaagactacg aacgcctgct ggatgccgtc
     4561 caggcggccg acatggatgg cgtcaagaaa tgcggcgatg cactggcccg cgcggtgcgg
     4621 acgtcggcca aggacattca cgacaagtac atcaacccgc cgctttcgac cgacttcggc
     4681 atcatgttcc tgccgtccga gggcctgtat tcggaaatcc tgcgccagcc cggcctggtg
     4741 ggcgagatcc agaacagtta ccgggtgatc gtggcgggcc cgaccacgct gtcatcgatc
     4801 ctttccagcc tgcgcgtcgg tttccagacc cttgccatcg aaaagcgttc gcacgaggtc
     4861 tggaaggtcc tggccgctgt caaaaccgag ttcgagacat tcggtgattt cctggaaaag
     4921 gttcagaagc agatcaacgc agcgtcaaag acgcttgaat cggccggcac ccgcacgaag
     4981 aagatgaaga gcaaactgtc gtcggtccag agcctgtccg ttgccgaagc cgcgaccatc
     5041 ctttccctgc ccgaaaaggg cgacgccatg cccgacgacg actacgaagc cgaagccgga
     5101 aacgctgaag gcgaacttgt ttgacccatt ggttttccgc tcagggcaga ttcgcgtaac
     5161 aaaaccggat cggaatcatt gccggttttg gtgataactg gtaatgtttt gttgccagtt
     5221 tgtttggaaa ctggtaagca attattgccg gtttgccgtg tctgcgattt gccggttatt
     5281 gtgtagggtg aagatatgaa ggaaatcctt aaggaaataa tccgggactc gcacgaggct
     5341 ccgatagtca cggggattcc ccggcgtttg aatacaccgg cgatcccggg caaggcgggc
     5401 gtcttcatgg gggttcgacg cagcggcaaa tcgacctata tgaatcagtt gatgcaaggt
     5461 ctgatagacg gcgggcttcc acgccagaac atactggccg tcaatttctt tgatgacagg
     5521 cttgccgagc ttcggcgcca gggtccggcc gccgtgatcg acgcttactg gtccaggttt
     5581 cccgaaaaaa agaatgtgga aaccatccat tgtttctttg atgaaattca agaggttgca
     5641 gattgggagc ccttcgtgga caggcttctg cgcaccgaaa attgtcaggt ttatctgacc
     5701 ggttcctctg cgaagatgtt gtcgtctgaa attgcgacgc agatgcgggg ccggtcgatt
     5761 ccctgggaag tattcccttt ttcattcccg gaatatcttg attcaagggg tattcaggca
     5821 ccggccgttc caaccagcag gcagcgcttc ctgatacgtc aggccttcga tgaattcatg
     5881 cttcgtggcg gttttccgga agtgaccggc tttgatcgca ggctgcgaac aaaagtacac
     5941 caggagtact tcggcgcaat catgtaccgc gatatcgtcg aaaggcataa tgtggcgcat
     6001 ccaaggatgc tgtcggaact gacgcgccgg ttgatcgaca acgccggttc gatgtattcc
     6061 cacaacagtt tgaccggctt tctgaagtcc accggtcaca aggcgaccaa ggcaattgtt
     6121 tccgacttcc tggaatggtt gcaggatgcg tatctgctct tttccgtgaa gctgttcgat
     6181 tcgtca
//
LOCUS       DAOPPA010000154         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_13917_length_6179_cov_4.920967, whole genome shotgun sequence.
ACCESSION   DAOPPA010000154 DAOPPA010000000
VERSION     DAOPPA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13917_length_6179_cov_4.920967"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKH54_06070"
     CDS             <1..769
                     /locus_tag="PKH54_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99489.1"
                     /translation="TAGWKETDSFIVDAVQADPEMAGEYYHVESMKFVGSSGRFAAFS
                     FVAESYLGGAHPVESRRLIVVDMEAGRVVDPLPMFGDRDLVREVLKDRFEAACVRKPS
                     GVGFLEGSGGKPVPVLLLTADFGVCEGQWALHPVDPPEGFGDVSQPAFEIRGHDLIRI
                     GRQPVATGVIDFRASPDGRRVVMEMAMGTMDEKSVPWSRKGSSRERTREIRFLMDKMD
                     RESVICRLPEVLSVQFPADSAAAEALARIVAAMNLSR"
     gene            complement(759..3362)
                     /locus_tag="PKH54_06075"
     CDS             complement(759..3362)
                     /locus_tag="PKH54_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOC99490.1"
                     /translation="MTFLAGLAVLVPFLVKEMAGARIDRAGASLKGVFRHDDIRIDGP
                     STLFLPSLLFQDPDGIEVSAADIKISVDPLAMLTGGRKITAVEVGRVRIVAGSREEPI
                     EPAKFAGRLMSRLLSRGDTVSAARETAPVESMHEPPRRSMLPDIRINSIVGRIDSSIV
                     SGELSSGRANLAPEPGAVDAATRTGRIEITFRPSGSTTAWRIDVNASVGPGFRPDSVV
                     ASVSPPVSMRLRGLAVQADSVTWKKREFRIDGIKASLPDSKRLPGEISAGQLMVRFKP
                     LGPGDVPAARLVPSDIERFFPNSLMLLLNAVAIQELRIARPVLDLAISAGDGTPPPAV
                     ASVSDPGTGSLPERITRTFGSISQRAESLRSSLSGLAQKYTGTRLAVNGATIRYVNTP
                     GRKDPISDHTLSNLDLVVERGSKSALLATLMFESPESSSLRNEATLMVGLDGSVDIGL
                     SASKLLLEPYRMLLPAWLQIDRSTAITDTDFRMKIQDGGRIEASGRMNLADAGILIRE
                     IATDAMTSVKLGVSGTATVDGTAGTISMPDSVASLGGIRIPFSFQASGLNVRPILKLD
                     ARIDRIDGKTLLESIPAAAIPILAGTRLDGTFAAGMRFAVDTGNISGLLFDFTPDMAD
                     LVTVDLGPAINLELLRSTFLHRIQDDNKVIVRRIGSDSPGWVPYARIPPFLVRALIAC
                     EDARFFSHEGFSKAGIQRSLKVNLERGGFFQGASTLSQQLVKNLFLSREKTLSRKLQE
                     AFITWQVEKNLPKEKILELYLNVIEWGPDVWGLKEAANHYFGKDPSALSVLEAAFLVL
                     IIPSPLKYHKFFEDGRVPAHFMKRIRELVDTLHSRGAVSDLEAISAAQQGIRFVSSGT
                     PAQVLDSEFSD"
     gene            complement(3469..3936)
                     /locus_tag="PKH54_06080"
     CDS             complement(3469..3936)
                     /locus_tag="PKH54_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99491.1"
                     /translation="MKRLVAVVLVFSMLGLGCYNTYQISGEQLEQLQVPPESGNSMTV
                     VDVKGQAVEVKDDTRLFVTSEGGRRYPITPFNFKQTESQLVASDRDYILARDGLREGA
                     QIEQVSTWKWALGVTGGVVLAGTIIGLIAWAAASSGTSQGGNSPAPAALQIGR"
     gene            complement(4028..4627)
                     /locus_tag="PKH54_06085"
     CDS             complement(4028..4627)
                     /locus_tag="PKH54_06085"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOC99492.1"
                     /translation="MPYFQQFYGLEVICGPMFSGKSEELIRRLRRAQIARQRVQVFKP
                     AIDNRYSQDCIVSHSELSLQARVVNSSAEILDLLDDRVEVVGIDEVQFFDQGIIRVCD
                     KLANLGKRVIVAGLDTDFRGIPFEPVPQLMAIADYVTKTLAVCHRCGAPASRTQRMSA
                     TRDRVVVGAVDCYEARCRRCHDPDLPDQLTMSLKDGTTL"
     gene            complement(4642..5685)
                     /locus_tag="PKH54_06090"
     CDS             complement(4642..5685)
                     /locus_tag="PKH54_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOC99493.1"
                     /translation="MENAGRRYGTHRVVEPKGTLPQAAWKVDNSLPIDPSEILIEVEM
                     LNIDAASFTQMKEAAGGDPDGIARIIMDTVENRGKQHNPVTGSGGMLVGKVAMKGAEY
                     DGPIEAAVGDRIATLVSLSLTPLKIRRIKKVFIDRDQVEIEGHAILFSSGIGERLPDD
                     FPRDAALAALDVAGAPAQVYRLVRPGMDVGIIGTGKSGLLCAAAARKVLGNSGRVFGV
                     VHQQSIIDEVSKYGYFDELILADATNPVDVFERINRLTGGRLLDVTINTTNVKSTEMA
                     TVLSTRDEGIAYFFNMATSFTAAALGAEGAGKDVTLLIGNGYAPGHARATLDLIRSDD
                     KLMSLFGAKYSNG"
     gene            complement(5702..>6179)
                     /locus_tag="PKH54_06095"
     CDS             complement(5702..>6179)
                     /locus_tag="PKH54_06095"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOC99494.1"
                     /translation="HTVFGEGPPNPMLMIVGESPGARDDELGRPFSGEPGELLTKIIN
                     AMGLDRERDCYLATVLKCRMPDESSPPPDLVGLCRPFLTRQVQIVKPRFLIAMGQTAA
                     MVLAGSPSISRLRGRLLMHDDIPMLVTYHPSALLRDPALKKLVWEDVKLIMARMKP"
BASE COUNT         1245 a         1794 c         1888 g         1252 t
ORIGIN      
        1 gaccgcgggc tggaaggaga cggacagctt catcgttgac gcggttcagg ccgatcccga
       61 aatggccggc gagtactacc acgtcgagtc catgaagttc gtcggctcgt cgggccgttt
      121 cgcggcattc agcttcgtgg ccgagtcata tcttggcggc gcgcatccgg ttgagtccag
      181 aaggctcatc gtggttgaca tggaggcggg cagggtcgtt gatcctctgc cgatgttcgg
      241 cgaccgcgat cttgtccgcg aggtcttgaa ggatcgcttt gaagccgcct gcgtcaggaa
      301 gccgtccggt gtcggatttc ttgaaggttc gggtggaaag ccggtgccgg ttctgctcct
      361 gaccgccgac ttcggggttt gtgaaggtca gtgggcgttg catccggtgg atccgcctga
      421 gggctttggg gatgtgtcac agccggcgtt cgagatccgc ggccacgatc tgattcgcat
      481 tggcaggcag ccggtcgcca ccggcgtcat cgatttccgg gcgtctccgg atgggcgccg
      541 ggtcgtgatg gagatggcga tgggaaccat ggatgaaaag tctgttccat ggtcgcgcaa
      601 aggctcttcc agggagcgga caagggaaat ccgcttcctg atggacaaaa tggacaggga
      661 gtccgtcatc tgcaggctcc cggaggtgct gtccgtgcag tttccggccg attcggcggc
      721 agccgaagcc cttgcaagga tcgtcgccgc catgaatctc agtcgctgaa ttccgaatca
      781 agtacctgcg caggagtccc ggagctgaca aatcgaatac cctgctgggc cgcggaaatg
      841 gcctccagat cgctcacggc cccccttgaa tggagcgtgt caaccagctc gcggattctc
      901 ttcatgaaat gcgcgggaac acggccgtct tcaaagaact tgtggtactt caacgggctc
      961 gggattataa ggacaaggaa cgcggcctcc agaaccgaaa gggccgacgg atccttgccg
     1021 aaatagtggt tggcggcttc cttaaggccc cacacgtccg gcccccactc aatcacgttg
     1081 aggtacagct cgagaatctt ttcctttggc aggttctttt caacctgcca tgtgatgaag
     1141 gcttcctgca gcttgcgtga aagagtcttc tcccgcgaga ggaagaggtt tttaacaagc
     1201 tgctgcgaga gcgtgctggc accctggaag aatccgccgc gctcgagatt gaccttcagg
     1261 gatcgctgga tgccggcttt cgaaaagcct tcgtgggaaa agaagcgggc atcctcgcag
     1321 gcaatcaggg cacgaaccag gaatggtgga atcctggcgt aaggaaccca gccgggcgag
     1381 tccgatccga ttctgcggac gatgaccttg ttgtcgtcct gtatcctgtg aaggaaggtg
     1441 ctccgaagga gttcgaggtt gatcgcgggg ccaagatcca cggttaccag atccgccatg
     1501 tccggagtga aatcgaagag gaggccgctt atattgcccg tatcgacggc gaatcgcatc
     1561 ccggcggcga aagtgccgtc aagcctggtg ccggcaagga tcggaatcgc cgccgccgga
     1621 atcgattcaa gaagcgtttt cccgtcaatc ctgtcgatgc gggcatcaag tttcagtatc
     1681 ggcctgacat tcaatccgct tgcctgaaac gagaacggga ttcgaatccc gccgagcgac
     1741 gcgacggagt ccggcatcga aatggttcct gcggtgccgt ccactgtggc ggtgcctgaa
     1801 actccaagct tgacggaggt catggcatcg gtcgcgatct cgcgaatcag gatgcccgcg
     1861 tcagccaggt tcatgcgacc agaagcctca atccttcctc catcttgaat tttcatgcgg
     1921 aagtcggtgt cggttatcgc ggtcgatcgg tcgatctgca gccacgccgg caacagcatc
     1981 cggtagggct caagcaggag cttcgaggcc gacagtccga tatcgaccga cccgtcgagg
     2041 ccgaccatca gggtcgcttc gtttcgcagc gacgatgatt ccggggattc aaacataagg
     2101 gttgccagaa gggccgattt gctgccgcgc tcgacaacaa gatcaagatt cgagagggtg
     2161 tggtcggaaa tcgggtcttt ccgtcctggt gtgttcacgt aacggatagt ggcgccattt
     2221 acggccagcc gcgttccggt gtacttctgt gcaagtccgg aaagggaact tcgcaggctt
     2281 tcggcccttt gggaaatcga cccgaacgta cgagtaatcc tttcaggcag ggatccggtc
     2341 ccgggatcgg aaacggatgc gactgccggc gggggcgtgc cgtcaccggc ggaaatggcc
     2401 agatccagca caggtctggc aatcctgagc tcctgaatgg cgactgcgtt cagcagaagc
     2461 atcagggaat tggggaaaaa gcgctcaata tcagacggca caagtcgggc ggccgggacg
     2521 tcgcccggtc ccaggggctt gaagcgaacc atcaattgac cggcggatat ctcgcccgga
     2581 agcctctttg agtcgggaag gctggccttg atgccgtcga tgcgaaattc acgcttcttc
     2641 caggtgaccg aatcggcctg cacggcgagt ccgcgaagcc gcatcgaaac cggcggggag
     2701 actgacgcga cgacgctgtc cggcctgaag cctggcccga ccgatgcgtt gacgtcgatg
     2761 cgccaggccg tcgtcgagcc ggatggcctg aaggtaattt caatccggcc tgttcttgtt
     2821 gcggcatcga cggcgcccgg ttccggggca agattcgctc tgccggaaga caattcgccc
     2881 gacacgatgg aactgtcgat tctgccgaca atactgttga tcctgatgtc gggaagcatg
     2941 gagcgcctgg gcggttcatg catcgattca accggggccg tttcacgggc ggccgaaacc
     3001 gtatcgccac ggctgagcag ccgcgacatc agcctgccag cgaactttgc gggttcaatc
     3061 ggctcctccc tggagccggc gacgatcctg acccgtccga cctcgacggc cgtgattttc
     3121 cgcccgccag taagcatggc aagtggatcc acggatatct tgatatcggc cgccgaaacc
     3181 tcgattccat caggatcctg gaacagcagc gatgggagga acagcgtcga tggtccgtca
     3241 atgcggatgt cgtcatgacg gaacacgcct ttcagcgagg cgccggccct gtcgatccgg
     3301 gccccggcca tttccttgac caggaacggc acaagaacgg caaggcccgc aaggaatgtc
     3361 acgattacaa gaaagacgac cgcgatcttc cggggaaacc ttaagtgtgg agttctgagt
     3421 cgggaagcgg atgaatcttg ggcgtcgggg ttcacatcat tccggatgct atctgccaat
     3481 ctgcagcgcg gcaggggcag ggctgttgcc gccctgggac gtgccgctgg aggctgcggc
     3541 ccaggcgatc aggccgatga tggttccggc caggacgacg ccaccggtga cgccaagagc
     3601 ccatttccac gtgctgacct gctcgatctg ggcaccttcg cgaaggccgt cacgagcgag
     3661 gatgtagtca cggtcggaag cgacaagctg cgattcggtc tgcttgaagt taaacggcgt
     3721 gatcgggtaa cgcctgccgc cttcggatgt gacgaaaagc cgggtgtcgt ccttgacctc
     3781 gactgcctga cccttgacgt caacaacggt catcgagttt ccggactccg gaggaacctg
     3841 cagttgttcg agctgttccc cggaaatctg gtaagtgttg tagcaaccaa gccccagcat
     3901 gctgaaaaca agaacgacgg caactaaacg cttcatcgag gccaacctca ccttataccc
     3961 aaagtccgcg ccgcattgta ttaacgcggg caaaaaaaga aaaggaattt ttgtccattc
     4021 ccgggtttta cagcgttgtg ccatccttca gcgacatcgt caactgatcg ggcagatccg
     4081 gatcgtgaca gcggcgacaa cgggcctcgt agcaatcgac ggcaccgaca acgacccgat
     4141 cccttgtggc cgacatgcgc tgggttcggc ttgccggcgc gccacagcga tggcagaccg
     4201 caagtgtctt cgtcacgtag tccgcaatcg ccatcaattg cggtaccggc tcaaacggaa
     4261 tacccctgaa atccgtgtcc agtccggcaa cgatcacccg tttgccaaga ttggccagtt
     4321 tgtcgcacac cctgatgatg ccctggtcaa agaactgaac ctcgtcgatg ccgacgacct
     4381 cgacgcggtc gtcaagcaga tccagtatct cggcactgga attcacgacc ctggcctgaa
     4441 gggaaagttc ggaatgggaa acaatgcagt cctgggagta tcggttgtcg atggccggct
     4501 tgaatacctg gacgcgctgg cgggcgatct gggcccttcg aagcctgcgg atcagttcct
     4561 cgctcttgcc cgagaacatc ggtccacaga tcacttcaag tccataaaac tgctggaaat
     4621 acggcatcct ggcccccgat ttcagccgtt ggaatacttc gcgccaaaaa gtgacatgag
     4681 cttatcgtcc gatctaatca ggtcaagggt ggcccgcgca tgtcccggcg cgtatccgtt
     4741 tccgatgagc agggtgacgt ccttgccggc gccttccgcg ccgagcgcgg ccgcggtgaa
     4801 ggacgtcgcc atgttgaaga agtatgcgat gccctcgtcc ctggtggaga gaacggtcgc
     4861 catctcggtt gatttcacgt tggtcgtgtt aatcgtcacg tccagcaggc gaccgccggt
     4921 cagccggttg attctttcga acacatcgac cggatttgtg gcgtcggcca gtatcagttc
     4981 gtcgaagtat ccatacttcg acacttcatc gatgatggac tgctggtgca cgacgccgaa
     5041 gacgcgaccg gagttgccaa gcacctttct cgcggcggcc gcgcagagca ggccggattt
     5101 cccggtaccg atgatgccca cgtccattcc cggcctgacc aggcgataga cctgagcggg
     5161 agccccggca acgtcaagcg cggcaagggc cgcgtccctg gggaagtcgt ccggaaggcg
     5221 ttcgccgatg ccgcttgaaa acaggatggc gtgtccttca atctccacct ggtcgcgatc
     5281 gatgaagacc tttttgatcc tgcgtatttt taacggggtc agggaaagcg agacaagtgt
     5341 cgcgatacgg tcgccgacag cggcctcgat cgggccgtca tactcggcgc ccttcatggc
     5401 gacctttccg accagcatgc cgccggaacc ggtaaccgga ttgtgttgtt tcccgcggtt
     5461 ttcgacggta tccatgatga ttcgggcgat gccgtcgggg tcgccgccgg cggcctcctt
     5521 catctgggta aaagaggcgg cgtcgatgtt gagcatctcg acttcgatca gaatttcgga
     5581 cggatcgatg ggcaacgagt tgtcaacctt ccatgccgcc tggggcagtg tccccttcgg
     5641 ctcgaccacc ctgtgcgttc cgtatcttct tcccgcgttt tccatttgca agctccatga
     5701 ttcatggctt catcctggcc atgatcagct tcacgtcttc ccacaccagc ttcttcaatg
     5761 ccgggtcgcg cagcagcgcg gacggatgat aagtcaccag catcgggatg tcatcatgca
     5821 tcaggaggcg tccgcgaagc ctgctgatgc tgggtgaacc tgcaagaacc attgcggcag
     5881 tctgacccat tgcaatcagg aagcgcggct tcacgatctg cacctggcgg gtgaggaacg
     5941 ggcggcacaa cccgaccaga tccggcggcg gcgacgattc gtcaggcatc cggcatttca
     6001 acaccgtcgc aaggtaacag tcgcgctctc gatccaggcc cattgcgttg attattttcg
     6061 tcaggagttc cccgggttca ccggagaatg gacgaccaag ctcatcgtcg cgcgcgcctg
     6121 gcgattcgcc gacgatcatc agcatcgggt ttggcggacc ttccccgaag acggtgtgc
//
LOCUS       DAOPPA010000155         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14029_length_6141_cov_3.303976, whole genome shotgun sequence.
ACCESSION   DAOPPA010000155 DAOPPA010000000
VERSION     DAOPPA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14029_length_6141_cov_3.303976"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKH54_06100"
     CDS             <1..325
                     /locus_tag="PKH54_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99495.1"
                     /translation="PTKSAIPVARPGTVTAQPAAQAQPAPGSASSGIQRPTAPSSPSP
                     TATAPERSPAPAPARAEPLSREKMDGLEAEADRDIKKKKGEDSSKRKKLDKDFDKEFE
                     EFEDF"
     gene            530..1066
                     /locus_tag="PKH54_06105"
     CDS             530..1066
                     /locus_tag="PKH54_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOC99496.1"
                     /translation="MRRGLLVALMMVFSFSMVACGGAEKKQDFSKAPDWYDAPMKGCG
                     VGSAKLRSVRDLARQSAVSSARADLSRGLSTQVDAMLKRYMAEGEAEGQDFSEELTKS
                     VVRDVTSQTLVGTRVAVTQVMNNEFYAMVCLDPETFADAFSRMDKLSQKAREALNKRA
                     KAEFDDMDEQIKKLNEQK"
     gene            1107..2036
                     /locus_tag="PKH54_06110"
     CDS             1107..2036
                     /locus_tag="PKH54_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99497.1"
                     /translation="MKKIIAIILFAAVCPLMLSCGSSGSTTKKSSLQEKALAEIRQAY
                     EVPLCWESVDDASAVGFIHQFAAKVVQGRISRDVQWIRTSDSDKLIAAEVFGNGAGAG
                     KDAGIITMTSAVPSDYAVWMAIADLFRNHRPEKPDVVEKRGYGVFLHTIRDDTRKLQL
                     DDLERTVRVTTDSLSFRVSPAVVCTSEFSMLAGSSAYVSPADAAIASLIKKGVIKGSE
                     EKRAQFFGILEGEVGAISLIQQVPGDSSGEYVLTARFDFDVASLFGFGGATEHVLVCR
                     SPNPMFASQLENYAFARQRQESGEYRYIATMAR"
     gene            complement(2134..4548)
                     /locus_tag="PKH54_06115"
     CDS             complement(2134..4548)
                     /locus_tag="PKH54_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC99498.1"
                     /translation="MTRNNSRTAAAEILPAIAMVALILAGTAGMAAEQGDSQPGETRI
                     PDDKRPVMIEIVPDVAVAGVGQPFRLDVIFRIAPGWHIYAPWPGNTGEPTTLKFGSDN
                     DGVQFGHTSFALPHIYDRQTLDLTAFGYSDGAVASSEVVVLEQPDAGEIELSVQSGWL
                     ACKQSCVGGKATAATRLKVAESASPSAGGTARDNLTPAATRLATASAWLSSVSYDDGI
                     WDIKYTLTGFQSVSEWVPQWLPGGECLVDNWGFERGGDGNESWIASVRLRGTDCLPFA
                     GGFLQATEPGSDKPENYFFGAGTNPANSPWTRATPDQATPDQQDAASLSADTSPPVVE
                     APAFSWYFLLLAFAGGLLLNIMPCVIPVIVPKLNHLVMTAVTYKERRERYRMLLANGL
                     AYAAGIFVLMMGLATTVVILKAAGHSVGWGFQFQEPGFLAFIISILMVMSLGMLGVFP
                     LQVASHTDDLQDLRKKYKTRMILGSFMTGLLVTILGTPCTAPMLGPAVGFAFMGGPAQ
                     IFAFFAALALGFSLPFVLLAVFPRWTDRLKNFRVSEESNRWSHGLAFFLLATMVWLIG
                     VLGSGFGIDAVVRILWFLLLLGAAAWFMGVSAVNFEEPAGRKLKKAESDLAADSSVDA
                     RERIELKRNLRKAHYRSVLRRRVITAGMLFAVIMVSAPMILSFQDGAAEGSAGLEKHD
                     GPVKWQKWSREAVEQARKAGKPVFVDVTASWCMNCKTNEKLILNRQQTADLFTEHGIV
                     PMIADFSRRDPSIAALIKQYQRAGVPVYLVYPPCEGEPAVLPELLTEGILTDAIKAAG
                     PARDCR"
     gene            4762..5928
                     /locus_tag="PKH54_06120"
     CDS             4762..5928
                     /locus_tag="PKH54_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99499.1"
                     /translation="MKGIPIHLIVVAMLLVSGCSGSNPAAAVSSSRAPDWVSVKPQDT
                     GSTLFFVTSGTSMASPGDAARNAMVQAASQIATYIGALVNSTSVSRQGSSASGDFSEF
                     SFEASIVGMPVVVRRVEQQGTWCTVASGRHECFVLVAYPVQEYERAKREVDAAQAEYR
                     RRQAESAARALSLFKLAQGDEHAGQFDKAMAALEQAIGIIDQFKIVIDVNDPMFTNSD
                     ILRRAAIDAVSRVSRNLTDSRLWVGVGVVVELDGTRDGQVEKKYFSTISGLLSRFSFK
                     ARAAGLTSEDATACLDGDADRVRSGSIGAGHLVVLELNSKFSSEIDKIYFARCSGRWA
                     LMESRTGRVLASGPVDGVKGGGVTREAALKEAVDKAWTASVAGGISGAIESLVR"
     gene            5943..>6141
                     /locus_tag="PKH54_06125"
     CDS             5943..>6141
                     /locus_tag="PKH54_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99500.1"
                     /translation="MSLPRFRLIIFTASILAIFLADGRSFANPGAQKVKVGDLVSAGK
                     LDIKLRDLGKGWFWRVSEMSYP"
BASE COUNT         1219 a         1784 c         1865 g         1273 t
ORIGIN      
        1 cccgacaaag tccgcgattc ccgtcgcccg tccaggcacg gtcacagccc agccggctgc
       61 ccaggcccag cccgcccccg ggtcggcatc cagcgggatt cagcgcccga cggcgccttc
      121 cagtccttcg ccgaccgcaa ccgcccccga gcgttcgccc gcgcccgcac cggcaagggc
      181 cgaaccgctt tcccgtgaaa agatggacgg actcgaggcc gaggccgaca gggacatcaa
      241 gaagaagaag ggcgaggata gttccaagcg caagaagctc gataaggact tcgacaagga
      301 attcgaggaa ttcgaggatt tctagggttg ccttccttcc gttcgcccgc atcggcatgt
      361 ccgcatggcg agagccgggt aacggcgtga ttgccggtcg ttccggcgtt tgaaattcgt
      421 ggggtgttga atttgtccgc gggaaatatt aatcttgcgg gcagattcag atgtttgcgt
      481 tctttttctt tgttcgtttc ttttttgcgc attgcgcagg aggtttcaga tgaggcgtgg
      541 tcttctggtt gctcttatga tggtattttc gttctcgatg gttgcgtgcg gcggcgcgga
      601 gaagaagcag gacttctcga aggctccgga ttggtatgat gcccccatga agggttgtgg
      661 tgtcggcagc gccaagcttc gcagcgttcg tgaccttgcg cgtcagtcgg ccgtttcttc
      721 ggcgcgcgcg gatctgtcca ggggcctcag cacccaggtt gacgcgatgc tgaagcgcta
      781 catggcggaa ggcgaagccg aaggccagga tttctccgaa gaactgacca agagcgtcgt
      841 ccgtgacgtg acctcccaga ccctggtcgg aacccgcgtg gccgttaccc aggtcatgaa
      901 caacgagttc tatgcgatgg tctgcctgga tcccgaaacc ttcgccgatg ctttctcccg
      961 catggacaag ctcagccaga aggcgcgcga agccctgaac aagagggcca aggcggaatt
     1021 cgacgacatg gacgagcaga tcaagaagct gaacgaacag aagtaattct tgtctcttga
     1081 cctgtctctt gccggaactt ccacccatga aaaagatcat cgcaattatc ctgtttgcgg
     1141 cagtttgtcc cttaatgctc tcgtgcggtt cgtcgggatc gaccacgaag aagagttccc
     1201 tgcaggaaaa ggccctggcg gaaatacggc aggcatatga agtcccgctt tgctgggaat
     1261 cagtcgatga tgcgtccgca gttggtttca tacaccagtt cgcggcaaag gtcgtgcaag
     1321 ggcggatcag tcgtgacgtt cagtggataa gaacttccga cagcgacaag ttgattgctg
     1381 ccgaagtttt tggcaacggg gcgggtgccg gcaaggacgc cggcatcatc acgatgacga
     1441 gcgcggttcc gtcagactat gcggtctgga tggcgatagc cgatcttttc aggaatcaca
     1501 ggccggagaa gccggacgtt gtcgagaagc gtggctatgg cgtcttcctg cacaccatca
     1561 gggacgacac caggaagctg caactggacg atctcgagcg aaccgtcagg gttacgacgg
     1621 actcgctttc gttcagggtc agtccggcag tggtctgcac gtcggagttt tccatgcttg
     1681 ctggaagcag cgcctatgtc agtccggcag acgccgccat cgcttccttg atcaagaaag
     1741 gcgtcattaa gggttccgag gaaaagcggg cacagttttt tgggattcta gagggtgaag
     1801 tcggtgcgat ttccttgatt cagcaggttc ccggggattc ttccggcgag tacgtcctga
     1861 ccgcccgttt cgacttcgac gttgcaagcc tttttggttt tggcggcgcg accgagcacg
     1921 tgctggtctg ccgttcgcca aacccgatgt ttgcgtcgca gcttgaaaac tatgcgttcg
     1981 caaggcagcg ccaggaaagc ggcgaataca ggtacattgc aacaatggcc cgctgagaca
     2041 ccgatccctc cactcgaagc acttgtccga atcttcgtgg cgcaagcgtg actcaacgct
     2101 gaaggcggca tcttcaagca atgggccggt cgcctatctg caatccctgg cgggtccggc
     2161 ggccttgatt gcgtccgtca ggattccctc ggtcaggagt tccggaagca cggccggttc
     2221 gccttcgcat ggcgggtaaa cgagatagac agggactccg gcacgctggt attgtttgat
     2281 cagagcggcg attgacgggt cgcgccggct gaagtcggcg atcatcggaa cgatcccgtg
     2341 ttcggtgaaa agatcggcgg tctgttggcg attaaggatc aacttctcat ttgtcttgca
     2401 gttcatgcac caggatgcgg tcacatccac gaacaccggc ttgcctgcct tccgggcctg
     2461 ctcgaccgct tcccgcgacc acttctgcca cttgacgggc ccgtcatgct tttcgagccc
     2521 ggccgaccct tcggcggcac catcctggaa gctgagtatc atgggggccg aaaccatgat
     2581 cacggcaaag agcatcccgg cggttatcac tcgccttcga agcacggatc gatagtgcgc
     2641 cttccgaaga ttccgcttca gttcgatgcg ctcgcgggca tccaccgaac tgtcggccgc
     2701 caggtcgctt tccgcctttt taagtttccg gccggccggt tcctcgaagt tgacggcgct
     2761 gacgcccatg aaccaggccg cggcgccaag cagcagcagg aaccacagga ttcgaacgac
     2821 ggcgtcgatc ccgaatccgc ttccaagaac gccaatcagc cagaccatcg tggccagcag
     2881 gaagaacgcc agtccgtgcg accaccggtt gctttcctcg ctgacccgga agtttttcag
     2941 gcgatccgtc catcgcggaa ataccgccag cagcacgaag ggaagggaaa atcccagggc
     3001 gagggcggcg aagaacgcaa aaatctgggc tggtccgccc atgaacgcga atccgaccgc
     3061 cggaccgagc atcggagccg tgcacggcgt gccaagtatc gtcacaagca ggcccgtcat
     3121 gaacgaaccg agtatcatcc tggtcttgta cttcttgcgg agatcctgca ggtcatcggt
     3181 gtgggaagcg acctgaagcg ggaaaacgcc aagcattcca agcgacataa ccatcaggat
     3241 ggagatgatg aacgcaagga atcccggctc ctggaactgg aatccccagc cgaccgagtg
     3301 gccggccgcc ttcaggatta caaccgtcgt cgcgagtccc atcatcagga cgaatatgcc
     3361 ggcggcgtag gccagtccgt tcgcaagcag catcctgtat cgctcacgcc gttccttgta
     3421 ggttaccgcc gtcatgacaa gatggttcag cttcggcacg atcacgggaa tgacgcaggg
     3481 catgatgttc aggagcagcc cgccggcaaa ggccagaagc aggaagtacc atgaaaaggc
     3541 gggcgcctcc acgacgggtg gcgaggtgtc ggccgacagc gaagccgcat cctgctgatc
     3601 cggtgtcgcc tgatccgggg ttgcacgcgt ccatggagag tttgccgggt tggttcccgc
     3661 cccgaagaag taattttccg gcttgtcgga tcccggctca gtggcctgca ggaatccgcc
     3721 ggcgaacgga aggcagtcgg tgcctcgaag cctgacactg gcgatccacg attcgttgcc
     3781 gtcgccgcca cgttcaaatc cccagttatc gaccagacat tccccaccgg gaagccactg
     3841 gggcacccat tccgaaactg actggaatcc ggtcagcgtg tactttatgt cccatatacc
     3901 gtcgtcatag ctcacggacg acagccatgc cgaagccgtc gccaggcgcg tcgcggccgg
     3961 cgtcaaattg tcgcgagccg ttccaccggc gcttggggat gccgattcgg ccaccttcaa
     4021 tctggtcgcg gccgtcgcct ttccgcccac gcaggattgc ttgcaggcca gccagcccga
     4081 ctggacggac agctcgattt ccccggcgtc gggctgctca agaacgacca cttccgaact
     4141 tgcaacggca ccgtcggagt acccgaaggc ggtcagatcc aaggtctgcc gatcataaat
     4201 atggggcaac gcgaagctgg tatgtccaaa ctgcactcca tcgttgtcgg atccgaactt
     4261 cagtgtggtc ggctccccgg tgtttccggg ccacggagcg tagatgtgcc atcccggagc
     4321 aatcctgaag atgacgtcaa gcctgaacgg ctgaccaacc ccggcaactg ccacgtcggg
     4381 cacgatttcg atcatgaccg gtcttttgtc gtccgggata cgtgtttcgc caggctgcga
     4441 gtcaccctgc tcggcggcca tgccggccgt tccggcaaga atgagcgcaa ccatcgcgat
     4501 ggccgggagt atttccgctg cggcagttct ggagttgttc cgagtcatcc gcaaagcacc
     4561 tgttgaataa gccggtcgct tgaatacatc ccgtcgaatt ttttgacaat atcttaattg
     4621 agccgattct ccccgcgcgg ttcgcaggga cggcggagat tgccttcctg tcacgccgcc
     4681 gccatggcag atgaggaaga tcccttgtcc gattgagccg gtgccgttaa aataccgccg
     4741 ggtcaagtct gggtaatgga cgtgaaaggg attccgatcc acttgatagt cgttgcgatg
     4801 ttgcttgttt ccggctgttc cggttcaaat ccggccgcgg cggtttcatc ttccagggcg
     4861 ccggactggg tcagcgtcaa gccccaggat accgggtcaa ccctgttctt cgtcacttcg
     4921 ggaacatcca tggcatcgcc cggggacgcg gccaggaacg ccatggttca ggcggcttcg
     4981 cagatcgcga cctacattgg cgcgctggtc aattccacca gcgtttcaag gcagggctcg
     5041 agtgcttcgg gtgatttttc agagttttcc ttcgaagctt ccatcgtcgg catgccggtc
     5101 gtcgtgcggc gggtcgagca gcagggcacg tggtgtaccg tcgcttccgg caggcacgag
     5161 tgcttcgtgc tggtcgcata tcccgtccag gaatacgaaa gggccaagcg ggaagtcgat
     5221 gcggcccagg ccgagtaccg tcggcgccag gccgaaagtg ccgcacgcgc gctgagcctt
     5281 ttcaaactgg cccagggcga cgagcacgcc ggccagttcg acaaggccat ggcggcactc
     5341 gaacaggcga tcgggatcat cgatcagttc aagatcgtga tcgatgtgaa cgaccccatg
     5401 ttcacgaaca gcgacattct gcggcgcgcc gccattgacg ccgtttcacg tgtttcacgc
     5461 aatttgaccg actccagatt gtgggttggc gtcggggttg tcgtcgaact tgacggcacc
     5521 agggacggcc aggtcgaaaa gaagtatttt tcgaccatca gcggccttct ttcccgcttc
     5581 agtttcaagg ccagggctgc cggattgaca tccgaggatg cgaccgcctg tcttgatggg
     5641 gacgcggaca gggtcaggtc gggatcgatc ggtgccggcc acctcgtcgt gcttgaactg
     5701 aattccaagt tttcttccga gatcgacaaa atatattttg cgcgctgttc aggccgctgg
     5761 gccttgatgg agtcaagaac cggcagggtt ctggcatcag gtccggtcga tggggtcaag
     5821 ggtggcggag ttacccgcga ggcagcattg aaggaagctg ttgacaaggc ttggacagct
     5881 tcggtcgccg gcggcatttc gggggcaatc gagtcccttg tcaggtagtc tggaggttcc
     5941 cgatgagtct tccccggttc aggctgatta tcttcactgc atcgatcctg gccatctttc
     6001 tggcggacgg caggtcgttc gcgaatcccg gcgcgcagaa ggttaaggtc ggtgatctgg
     6061 tctctgccgg aaagcttgac atcaagttga gggatcttgg caagggttgg ttctggcgcg
     6121 tttccgaaat gtcatacccc g
//
LOCUS       DAOPPA010000156         6115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14103_length_6115_cov_3.818647, whole genome shotgun sequence.
ACCESSION   DAOPPA010000156 DAOPPA010000000
VERSION     DAOPPA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14103_length_6115_cov_3.818647"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /gene="dnaE"
                     /locus_tag="PKH54_06130"
     CDS             <1..1579
                     /gene="dnaE"
                     /locus_tag="PKH54_06130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOC99501.1"
                     /translation="MDEEFIAVKHGQKKAQYAHPLLEPILKDTYGTILYQEQVMLIAS
                     RLGGFSLGQADILRKGMGKKDHAAIERQKDPFIKGCSERGIPTSVSRELFEKLEKFGS
                     YGFNKSHSVAYAYVSYQMAYMKTHFPVEFICAVLYAKKGNQSDVMRFIYEAQESGIPV
                     LPPDVNRSQADFTVEDVKDPDGTTKGAIRFGLTAVKGVGTTAIALVLKAREDGPFESV
                     ADFLARIDKQKVNRKVVESLIKAGAFDAFGHTRRSLAEGINQLFDAAARLSEDRESGQ
                     GGLFDLSEDTSVKTVSGPPPLPEWPQREKLALERDALGYYVTGHPIHEYRDSLAKFDV
                     LRLSDMDETMSGKNVQVAGMVLTVEEKISKKTGDPMAFLQIDDGTGQLECSVFSKHYH
                     KWECVRNSPEPLLFSGRLDVETNIETEESRYRLLCDGIQRLDDARRLLTKSVRIHVDA
                     CAARSQTIDEMYRIVKNHPGPTPIEIEMVLPGTGRMMIRANSRWAVDPSEELLRELRQ
                     LLDGHECGCGPTPIEIN"
     gene            complement(1611..5153)
                     /locus_tag="PKH54_06135"
     CDS             complement(1611..5153)
                     /locus_tag="PKH54_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99502.1"
                     /translation="MAQKCPICKCMVPTDGFMPGETVTCPDCGTGIVVDPQLPGAAAR
                     GKAIQDAEPQASGKGRSAYGSAKAQVNSAVGSYSFKFGDKKAPAERVFDPKAGSSSRA
                     TPPRPEAAPSAGRQAAVRPEQPKPDPKAAAAADFFSRPAEGGWNPFQGAPKAAPVEKK
                     QSVDPDMTIGFGDGADFEFKRPDDSRRPDNDTVKQAPVDSFNFFDPQPQASQPAQPAP
                     RQDSGDLGFDLDAVLGSDVASGIGAPSQADPFGWGGDIQHGNPPGVPQEDVWTTAETV
                     PLSVDHLGRNDFGGTGDFGDAADFDESVSSQLDGGQDGIDPNQLLSDDFDLKGGLMAD
                     LIYDVPPDEAAPLKALAVDAPGKGSKARARAGRSQRKTTTTPQRRRLGRVLIIPLVVV
                     LIGAILGLTDYGYFGINLLSGGAKPSNSYYKTIKSDIVVVNDSREAFIERIATLERLY
                     RDDDSDENLVELVLTMNRYKERYPSAFSSDSRMTARLDQLKQLAAGKDARTGNMTKVM
                     DLVGQGKYTEARAMLDSMVASSAQDVDVLYYYGKIALATDKPDEAQKYFELALIKNPG
                     LISAKYFLAQAFWNQKKTVEAKALLSEIMAKEATHLPSRILSATIALDESSVDEAANL
                     AQHVIAAGTPGSDTEEIFQAHRIMARVHLLGGKPAERLASLRAALLLKPAHEETAVEA
                     GEILLRTGKRGEALDALTPCREHGCVSLAFLLVFAQAAYADDKVDVARAAIAQGEQRY
                     PESPRFGVIEGSYHLRERRFRSAEDALEKSIKLDPKHVEAYLLISDAFMEEGMADNAA
                     SYLEKGLAQIGPEARLLIKLADIRIARKELPEAEAALRQAVQIDQNSDSAQLKLGMVV
                     KDQGRNDEAASILNVLEKRRALDFNGSVALAEAYLEMKNFERARDILQQLYSANPDSP
                     EAGNAFGRALGEGREYQKAIEVLSRVETRFPNNSRAPYLLGNVLMAQGKFQDAINSFE
                     KAIKLDFSRHEYRLAASAAYIALGSVDSDVEARKHLDLIITDYQSGKIAPEDQNPEAY
                     LMRGRILFSRQKYGQAMKDFEAALSLAPSRRDIMVEFGRSLFEMQRYDDATAYFKQVL
                     ASDSLNPVANYFMGRIHLRDGDTDTAQKFLTNAVKRNPGMFADAHKLLGFIYKDKGLR
                     SLARDSFKAFLKHTKDRQAADAKEITRILEKGSY"
     gene            5315..5677
                     /locus_tag="PKH54_06140"
     CDS             5315..5677
                     /locus_tag="PKH54_06140"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOC99503.1"
                     /translation="MDTGPKPAGSIGDANAILGKGAEFEGKLAFEGTVRIDGKFSGEI
                     SSEGTLVIGEGARVTAEVSVGTLVVHGDVIGNVKAVNLVDLHANGRLRGNIRTSGLAV
                     HQGAIFDGNCSMTAPTDA"
     gene            5677..>6115
                     /locus_tag="PKH54_06145"
     CDS             5677..>6115
                     /locus_tag="PKH54_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99504.1"
                     /translation="MRGEGLPHGARALKTVAIVGLPVVAILLGLGGCDRVPEDRRGHS
                     GENNAASPHSGSAVQWRPEGLEAVRFLAAVHGFAERAPDASLLAAIEPFLKTSVCRRM
                     AGAMQPADIRALLSRKPSSVVIKGLRFVVSFADPAAGASAALGS"
BASE COUNT         1226 a         1831 c         1832 g         1226 t
ORIGIN      
        1 gatggacgag gaattcattg cggtaaagca cggccagaag aaagcccagt atgcccaccc
       61 gctgctcgaa ccaatcctga aggacaccta tggaaccatc ctttaccagg aacaggtgat
      121 gctgatcgct tccaggcttg gcggcttctc gcttggtcaa gcagacatcc tgcgcaaagg
      181 catgggcaag aaggatcacg ccgcaatcga gcgtcaaaag gatccattca tcaaggggtg
      241 cagcgaacgc gggattccga catcggtttc gcgggaactg ttcgagaagc tcgaaaagtt
      301 cggttcatac ggattcaaca aaagccacag cgtcgcttac gcctacgttt cgtaccagat
      361 ggcgtacatg aagacccact tcccggtgga attcatctgc gcggtgctgt acgcgaagaa
      421 gggcaaccag tccgacgtga tgaggttcat ttacgaggcg caggaatccg ggattccggt
      481 gcttccgccc gatgtcaaca ggtcccaggc cgatttcacg gtcgaggacg tcaaggatcc
      541 ggacggcacg acaaaaggcg cgatcaggtt cggactgacg gcggtcaagg gtgttggaac
      601 caccgcaatc gctctggtgc tgaaggcccg cgaagatggc ccgttcgaat ccgtggcgga
      661 cttcctggcc aggatcgaca agcagaaggt caaccggaag gtcgtcgaaa gcctgatcaa
      721 ggccggcgcg tttgacgcct tcgggcacac caggcgatcg cttgccgaag gaatcaacca
      781 attgttcgac gccgcggccc gcctgagcga ggaccgggag tccggccagg gcggactttt
      841 cgacctgtcg gaagacacct cggtaaagac ggtttccggg ccaccgcccc ttcccgaatg
      901 gccccagcgc gaaaagcttg cgcttgaacg cgacgcgctc ggatattacg tcacaggcca
      961 cccgatacac gaatacaggg acagcctcgc gaaattcgac gtcctgcgcc tgagcgacat
     1021 ggacgaaacc atgtcgggca aaaacgtcca ggtcgccggc atggttctca cggtcgagga
     1081 aaaaatttcc aaaaagaccg gcgatccgat ggccttcctg cagatcgacg acggaaccgg
     1141 ccagcttgaa tgcagcgtgt tctccaagca ctaccacaag tgggaatgcg ttcggaacag
     1201 ccccgaacca ctcctctttt ccgggcgact tgatgtcgag acaaacatcg agaccgaaga
     1261 gtcaaggtat cgcctgctgt gcgacggaat tcaaagactg gacgatgcca ggcgtctgtt
     1321 gacgaagtcg gtcaggatac atgtcgatgc ctgcgcggcg cgttcgcaaa caatcgacga
     1381 gatgtaccgc atcgtcaaga accacccagg ccccaccccg attgaaatcg agatggttct
     1441 gcccgggact ggccgcatga tgattcgcgc caattccaga tgggcggtcg atccgtccga
     1501 ggaacttctg cgcgagctgc gccagcttct ggatggccat gaatgcggct gcgggccgac
     1561 cccaatcgaa atcaactgat tccggcccgg gtcgggtcga ggtgtgaatc ctagtacgag
     1621 cccttttcca ggatcctggt gatttccttc gcgtcggcgg cctggcgatc cttcgtgtgt
     1681 ttcaggaaag ccttgaaact gtcccttgcc agcgagcgca gtcccttgtc cttgtagatg
     1741 aatccaagca gcttgtgcgc gtccgcgaac atcccgggat tgcgcttgac cgcgttggta
     1801 aggaacttct gtgccgtgtc agtgtccccg tcgcgaagat ggatcctgcc catgaagtag
     1861 ttggcgaccg gattcagcga atcgctggcg agaacctgct tgaagtatgc cgtcgcatcg
     1921 tcataccgct gcatctcgaa cagcgatcgc ccgaactcaa ccatgatgtc gcgacgcgat
     1981 ggagccaggg aaagagccgc ctcgaaatcc ttcatggcct ggccgtactt ctggcgggaa
     2041 aagagtatcc gcccgcgcat caggtacgct tcgggattct gatcctccgg ggcgatcttc
     2101 ccggactggt aatcggtgat aatcagatcc aggtgcttgc gggcttccac gtcggaatcg
     2161 acgctaccca gcgcgatgta ggccgccgag gccgccagcc ggtattcatg cctcgagaaa
     2221 tccagcttga tggccttttc aaagctgttt atcgcgtcct ggaacttgcc ctgggccatc
     2281 aggacgtttc caagaaggta cggggccctt gaattattcg gaaagcgggt ttcgaccctg
     2341 gacaacacct cgatggcctt ctgatattcg cgcccttccc caagtgcccg gccgaaggcg
     2401 ttaccggcct cgggactgtc gggattggcg gagtagagtt gctgaaggat gtcgcgggcg
     2461 cgctcgaaat tcttcatttc cagataagct tcagccagcg cgacgctgcc attgaaatca
     2521 agcgcgcggc gcttttccag gacgttcagg atggaagcgg cctcatcgtt acgcccctga
     2581 tcctttacga ccattcccag cttcaactgc gccgagtcac tgttctggtc aatctgcact
     2641 gcctgccgca gcgcggcttc ggcttcaggc aattccttgc gggcaatcct gatatcggcc
     2701 agcttgataa gaagccgcgc ctccggacca atctgcgcca gccccttttc aagataggag
     2761 gcggcgttgt cggccatgcc ttcctccatg aaggcatcgg aaatcagaag gtatgcctcg
     2821 acatgcttcg gatccagctt gatggacttc tcgagcgcgt cctcggccga ccggaaccgc
     2881 ctctcgcgaa gatggtagct gccttcgatg acgccaaagc gaggcgactc gggataacgt
     2941 tgttcgccct gggctattgc cgcccgcgcg acatcgacct tgtcgtcggc ataggcagcc
     3001 tgggcaaaca ccaggagaaa tgccagggaa acacatccgt gttcacgaca tggggtaaga
     3061 gcgtcaagcg cctcgccgcg ttttccggtt cgcaggagaa tttcgccggc ctcgacggcg
     3121 gtctcctcgt gcgcgggctt gagcagcagt gcggcccgca gcgatgccag gcgctcggcc
     3181 ggcttcccgc caagaagatg gacgcgggcc atgattcgat gtgcctggaa aatctcctcg
     3241 gtatcggagc ctggggtgcc ggcggcgatg acgtgctgag caagatttgc cgcttcatcg
     3301 acgctggact cgtccagggc gatagtcgcc gaaaggatgc gactgggaag gtgcgtggcc
     3361 tccttggcca tgatttccga cagaagcgcc ttcgcctcga cggtcttctt ctggttccag
     3421 aatgcctggg caagaaagta ctttgcggaa atcaggccgg gattcttgat cagggccagt
     3481 tcgaagtact tctgcgcctc gtcgggctta tccgtcgcca gcgcaatctt gccgtagtag
     3541 taaaggacat caacatcctg ggcggacgac gccaccatgc tgtcgagcat ggccctggcc
     3601 tcggtgtact tgccctgccc cacaagatcc atgacctttg tcatattgcc ggttcgggca
     3661 tccttgccgg ccgcgagctg cttgagttga tccagcctgg ccgtcatccg tgagtcactt
     3721 gaaaacgcgg agggatagcg ctccttgtac cggttcatgg tcagaaccag ttcgaccagg
     3781 ttttcgtccg agtcgtcatc gcgatacaaa cgttcaagcg ttgcaatcct ttcaatgaag
     3841 gcttcccttg aatcgttgac gacgacgatg tcggacttga tcgtcttgta atacgagtta
     3901 cttggcttgg ccccgcctga cagcaggttg atgccgaagt agccgtagtc ggtcagaccg
     3961 aggatggcac caatgagcac gaccaccagg gggataatca ggactcggcc aagccgccgg
     4021 cgctgtgggg tcgtcgtcgt cttgcgctgg gaacgaccgg cacgggcccg tgccttggag
     4081 cccttccccg gcgcatcgac cgcaagcgcc ttcagggggg ccgcttcgtc cggaggcacg
     4141 tcgtaaatca ggtcggccat caggccgcct ttcaaatcga aatcatcgga aaggagttga
     4201 ttcgggtcga ttccgtcctg accgccgtca agctgtgaac tcacgctctc gtcgaaatcg
     4261 gccgcatcgc cgaaatcccc ggttccgcca aagtcgttcc ggccaaggtg atcaaccgaa
     4321 agtggaaccg tctcggccgt tgtccacacg tcctcctgag gaacgccggg cgggttgccg
     4381 tgctggatgt cgccgcccca gccgaacgga tcggcctgcg acggcgcgcc gattccagag
     4441 gcgacgtcgg aaccgagaac ggcatccaga tcaaaaccaa gatcacccga atcctgccgg
     4501 ggcgctggct gggcaggctg ggatgcctgc ggctgcgggt cgaagaaatt gaaggaatcg
     4561 accggagcct gcttgacggt atcgttgtcc ggccgcctgg aatcgtccgg tcgcttgaat
     4621 tcaaaatcag caccgtcgcc gaaaccgatg gtcatgtcag ggtcaacgga ttgcttcttc
     4681 tcgaccggtg cggccttcgg cgccccctgg aaggggttcc agccaccttc ggccggtcgg
     4741 gagaaaaagt cggctgccgc agccgccttc ggatccggtt taggctgctc gggccggaca
     4801 gccgcctgac gaccagcact cggagcagcc tccggccgtg gcggtgtcgc cctggatgat
     4861 gaaccggctt tcggatcgaa aacgcgttca gccggcgcct ttttgtcgcc aaacttaaag
     4921 gagtatgaac caaccgccga attcacctga gccttggccg agccgtaggc cgatcgcccc
     4981 tttccggatg cctgaggttc ggcatcctgg attgccttgc ctcgcgccgc cgccccgggg
     5041 agttgcggat ccacgacgat accggttccg cagtccggac aagtgaccgt ttcacctggc
     5101 atgaagccgt ctgttggaac catgcatttg cagattggac acttctgagc catcgcaacc
     5161 tccagttgcg cccgatgata ctactattct tccgcgcgat gaaccgtaaa tggcaaattg
     5221 acggcgtcct gcaagtgttc taaattctgt ggcggattat cgaagcactt cactcgttga
     5281 agctgacagg cttttttgca ccgcgggggt tttcatggac actggaccca agccggccgg
     5341 atcgattggc gacgccaacg ccattcttgg aaaaggcgcc gaatttgaag ggaaacttgc
     5401 gttcgagggg actgtccgga ttgacggcaa gttctctggc gagatttctt ccgaaggaac
     5461 cctggtgatc ggggaaggcg ccagggtaac cgccgaagtt tcggttggca cacttgtcgt
     5521 ccacggagac gtgatcggaa acgtcaaggc cgtcaacctg gtcgatctgc acgccaatgg
     5581 caggcttcgc ggtaacataa ggacatccgg gctcgcggtt caccagggtg ccatcttcga
     5641 cggcaactgc tcgatgaccg ccccgaccga cgcttgatgc gcggcgaagg cctcccccac
     5701 ggagccaggg ccttgaagac tgtcgcgata gttggactgc ccgttgttgc catcctgctc
     5761 gggttgggtg gatgcgatcg tgtacctgaa gaccgccggg gccattcagg cgaaaacaat
     5821 gccgcatcgc cgcactccgg atctgccgtt caatggcggc ccgagggcct ggaggccgtc
     5881 cgcttcctgg ccgccgtcca tggatttgcc gagcgcgctc cggacgccag ccttctcgcg
     5941 gcgattgagc cgttcctcaa gacgtcggtc tgccgcagaa tggctggcgc gatgcagccc
     6001 gccgacattc gagccctgct ttcccgcaaa ccgtcctcgg tggtgatcaa ggggctcagg
     6061 ttcgtggtca gctttgcgga tcctgccgcg ggtgcctccg cggcactggg ttcag
//
LOCUS       DAOPPA010000157         6115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14104_length_6115_cov_3.724752, whole genome shotgun sequence.
ACCESSION   DAOPPA010000157 DAOPPA010000000
VERSION     DAOPPA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14104_length_6115_cov_3.724752"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKH54_06150"
     CDS             complement(<1..332)
                     /locus_tag="PKH54_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99505.1"
                     /translation="MKTVRLLAWDKSNNRNSFQPEIDYLVPDTMTVGRMLMLASASGY
                     DSILVAGGEPALRRDLPRIVATAASLGLQVALSTNARIFSCAGAAAGFAAAGLKRAVV
                     CLAGADPAV"
     gene            564..1787
                     /locus_tag="PKH54_06155"
     CDS             564..1787
                     /locus_tag="PKH54_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOC99506.1"
                     /translation="MAARGGNAIIGQSGGPTAVINQSLVGVVEALVRVPEVGRILGAR
                     HGVKGILGEDFIDLRLEAADVLERVAATPSSALGSVRKKPTPEECRRMFDVMQKHDVR
                     YFFYIGGNDSAETTHIVNEEARSAGYELRCFHVPKTVDNDLRVNDHTPGYGSAARFVA
                     CALMGDNLDNRALPGVKIDVIMGRNAGFLTAASALGRRYPDDGPHLIYLPERPVTRDR
                     ICSDVRDVYNRLGRCVVAVSEGIIDADDPAGRTFTEVILSEAGPIEQDTHGNVQLSGS
                     GALGDWLANLVKKNVGEKLRVRADTFGYLQRSFPGVVSPVDAAEARECGRKAVEFAVG
                     GSWLDGSVAMIREPGEEYRIRYERTELRNVAKETRSLPDDFITLEGNNVTVEFVRYAL
                     PLVGELPENCRLAGW"
     gene            1833..2714
                     /locus_tag="PKH54_06160"
     CDS             1833..2714
                     /locus_tag="PKH54_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOC99507.1"
                     /translation="MIDTHCHLADPVFADTLAEVVATARDAGVRGFVVPSVDVVSSAD
                     SVACCRNIRGAVAAIGIHPMWGRFDSRSGMELAVADIASLATGNRDVVGAIGEIGLDP
                     NLLPTVVEDRDRVQDSSGEDAIGRSIDLMIAQMELARWLGLPILLHFRGRVEGAPGPT
                     ISFQKLVDVLERFGPVRPGGILHAFSGSDQVASRLRRLGWLRGVAGTVSRPSATRLSE
                     FIAKTPLTDIVLETDAPFIANSLHPRGQVVPADLALVGHAIAGLHGVPFEHVIRQTDI
                     NAESLFSAGGSRNWRDI"
     gene            2719..3534
                     /locus_tag="PKH54_06165"
     CDS             2719..3534
                     /locus_tag="PKH54_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOC99508.1"
                     /translation="MDGRLECGKWQEGSGGEVPGAFRRLELAVGPEGIKALGNARVAV
                     IGLGAVGSFAVEALARSGVGYLRLVDFDIVQASNINRQIFALSSTVGLPKADVARARV
                     LDIFPGCRVDTRLEFFSEETASSILDAPADEPLDFVIDAIDSLLSKVNLIVACRNRGI
                     PIVTSMGAADRMDQSAVHVTDISESRNCPLAKHVRKRLHRRGIFDGVAAVWSSEGPNS
                     EGAAGGESPDADEEPARGRQRMPLPSMAPIPGIFGLSAANWVIRGILEKTKVG"
     gene            3569..5734
                     /locus_tag="PKH54_06170"
     CDS             3569..5734
                     /locus_tag="PKH54_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOC99509.1"
                     /translation="MNILGISAFYHDSAACLVIDGRIVAAAQEERFTRIRHDPAFPYN
                     AAMYCLSRAGLQPAAIDMVVFYEKPLLKLERILQNAVDVAPAGFVPFCRSMPSWFSEK
                     LNLPRILKKELGFRGPLMFAIHHQAHAASAFYPSPFNEAAILTVDGVGEWSTGSIGVG
                     RGNEIELRKETRYPHSPGLLYSAFTEFLGFKVNSDEYKVMGLAPYGEPVFVDAILNEM
                     VHVDEEGGFELNMDFFRFQFGSRTAGPGFSRLAGCPPRNPESRIEQVHMDLARSIQAV
                     IEIIMLRQARFARELTGMNNLCMAGGVALNCVANGKILKERIFDDIWIQPAAGDAGGA
                     VGAALIGWYSTEGASRVVDENDSMQGALLGPEFDVAGIEAALIEAGLPFERCGDDIYR
                     QIAAGLAEGQVIARFSGRMEFGPRALGSRSILADARNPAMQRIVNEKIKFREGFRPFA
                     PAVLLDHADAWFDLDRRSPYMLLVDQVASRQLLDRPAIEASGLDRLKIPMSTIPAVTH
                     VDGSARIQTVEPSTQPDFSRIIDAFWQRTGCPVVLNTSFNLRGQPIVCTPSDAVQTFL
                     ACDIDALAAGPFMARKPFGWTRRPLPRPTAFRRPRQRGELRRFGIENGIFFGLLGILA
                     WLAPDSPFRLGSFLAWGVSGLALLAGLAAPGILRAPEDRLNRAAARMNGIAGFILFGL
                     VFVLVLTPTALLRRIARAKREPGGWRDFFRDHGGLENMY"
     gene            5753..5902
                     /locus_tag="PKH54_06175"
     CDS             5753..5902
                     /locus_tag="PKH54_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOC99510.1"
                     /translation="MNLVREIWGWARRRKKYWLIPLVLALVLVGALIVVASATSVSPF
                     IYPLF"
     gene            complement(5907..>6115)
                     /locus_tag="PKH54_06180"
     CDS             complement(5907..>6115)
                     /locus_tag="PKH54_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99511.1"
                     /translation="SSHDIRMLFLHQLLLQAEADYMEIRYDPCCHFNEKGHRLLADIF
                     LRIVAEDSDRGAPKEPAETGADVE"
BASE COUNT         1170 a         1825 c         1937 g         1183 t
ORIGIN      
        1 acggccggat ccgcgccggc caggcagaca acagcccgtt tcaggccggc cgccgcaaaa
       61 cccgccgcgg cgccggcaca ggaaaagatc cgggcattcg tgcttaaggc gacctgaagt
      121 ccaagactgg cggcggtcgc gacaatccgg ggaagatcgc gacgaagtgc cggttcgccg
      181 cccgccacga gaatcgagtc gtagcccgat gcggacgcga gcatcagcat ccgcccgacg
      241 gtcatcgtat cgggaaccag ataatcaatc tctggttgaa acgagttcct gttattcgat
      301 ttgtcccagg ccagcagacg cactgtcttc atcttcggac tccaattggc gccaaaaaac
      361 cggcgccgcg gccagatgtt acaacggaag aacccggcag tcacacaaca aatgggattt
      421 gccgcccgga ttgattcaaa gagttttttt tccgcgacaa ggtcaacata cgaataaaat
      481 cgttgactaa tggcccgtgt gactgaaaaa ttcagccgcg attttcggta tcccgactca
      541 acgggaaaac tggagaaaaa ttcatggcag caagaggcgg aaacgcgatt attggtcagt
      601 ccggaggccc gacggccgtt atcaaccaga gccttgtggg agtggtggag gccctggtga
      661 gggttcccga ggtgggtcgg attcttggcg cccgccacgg cgtgaaggga atacttggcg
      721 aggatttcat cgacttgagg ctggaagcgg ccgacgtgct tgaaagggtc gccgcgactc
      781 cgtcttccgc gctcggttcc gtccggaaaa agccgactcc cgaggaatgc cgcaggatgt
      841 tcgacgtgat gcagaagcac gacgtaaggt acttcttcta catcggtgga aacgactcgg
      901 cggaaacgac ccacatcgtg aacgaggaag ccagatccgc tggatacgaa ctgcgctgct
      961 ttcacgtgcc gaagacggtt gacaacgacc tgcgggtcaa cgaccacacg cccggttacg
     1021 gcagcgcggc caggttcgtc gcgtgcgccc tgatggggga caacctggac aaccgggcac
     1081 ttcccggggt caagattgac gtgataatgg gtcggaacgc cggattcctc actgctgcct
     1141 cggcgcttgg caggcgctac ccggacgatg gcccccacct gatttacctt ccggagcgcc
     1201 ccgtcacgcg cgaccggata tgcagcgatg ttcgggatgt ctataaccgg ctgggaaggt
     1261 gtgtcgtggc ggtttccgag ggcatcatcg acgcggatga tcccgccggc cggacattca
     1321 ccgaagtcat cctttccgag gccggaccga tcgagcagga cacccacgga aacgtccagc
     1381 tttcgggatc cggggccctt ggcgactggc ttgccaatct ggtcaagaag aacgtcgggg
     1441 aaaagctgcg ggtacgcgcc gacactttcg gatatcttca gcgttcattc ccgggcgtgg
     1501 tcagtccggt tgacgcggcc gaggcccggg aatgcgggcg taaggctgtg gaattcgccg
     1561 tcggcggcag ctggctggac ggatcggttg cgatgatccg cgaaccgggc gaagagtacc
     1621 gaataaggta tgaacgcacg gaactccgga acgtggcaaa ggaaaccagg agcctgccgg
     1681 acgacttcat aacgcttgag ggcaacaacg tcaccgtcga gttcgtccga tacgcacttc
     1741 ccctggtcgg tgaacttccg gaaaactgcc gtctcgccgg gtggtagaat cccctggtgc
     1801 cacgattcaa accggactga aggttccatt cgatgatcga cacgcactgc caccttgccg
     1861 atccagtttt cgcggacacg ctggccgaag tggtcgccac ggccagggac gcgggtgtgc
     1921 gcggattcgt ggtgccatcg gtggatgtcg tctcgtcggc cgattccgtg gcctgctgcc
     1981 gaaacatcag gggtgccgtc gcggcgatcg ggatacatcc gatgtggggc cgcttcgaca
     2041 gtcgttccgg gatggaactg gccgtcgccg atatcgcctc gctggcgacg gggaaccggg
     2101 acgtggttgg cgcaatcggc gaaatcggac ttgacccgaa cctgctgccg accgtcgtcg
     2161 aagaccgcga tcgggtccag gattcgagcg gggaagacgc catcgggcgc tcaatcgacc
     2221 tgatgatcgc ccagatggag cttgcgcgct ggcttggcct tccaatactt cttcatttcc
     2281 gtggccgggt ggaaggcgcc cccggcccga ccatatcttt tcagaagctt gtcgacgtcc
     2341 tggaaaggtt cgggcccgtc cgtccaggtg gaatccttca tgcgttcagc ggctccgacc
     2401 aggtcgcctc caggcttcgc aggctcgggt ggctccgtgg cgtcgcggga acggtttcaa
     2461 gaccttccgc gacccggcta tcggaattta ttgcaaaaac cccgctgacc gacatcgtcc
     2521 ttgaaaccga cgcgccgttc attgcaaact cgcttcaccc cagggggcag gtggttccag
     2581 cggacctggc gctggtcggc cacgcgatcg ccggtctgca cggcgtgccg ttcgaacacg
     2641 tgatccggca gaccgacatc aacgcggaat cgctcttttc agcgggcggt tcgcggaact
     2701 ggagggatat ctgaattgat ggacggacgc cttgaatgcg gaaagtggca ggaaggttcg
     2761 ggcggggaag tccccggggc cttccgcagg ctggaactcg cggtcggccc ggaagggatt
     2821 aaggctctgg gcaacgccag ggtcgccgtc atcggacttg gcgcggttgg ttccttcgcg
     2881 gtcgaggcgc tggcgcgttc aggagtcggg tatctccgcc tggtcgattt cgacatcgtc
     2941 caggcatcca acatcaatcg ccagattttc gcgctgtcct cgaccgtcgg cctgccgaag
     3001 gctgatgtgg caagggccag ggtgctggac atattccctg gttgccgggt cgataccaga
     3061 ctggaatttt tcagcgagga aaccgcttcg tcaatactgg acgctccggc tgacgagccg
     3121 ctggacttcg tgatcgacgc gattgacagt ctgctgtcca aggtgaacct gatcgtggcc
     3181 tgccggaacc gcggcatccc gattgtcacg tcgatggggg ccgccgaccg tatggatcag
     3241 tcggcggttc atgtcacgga catatccgaa tccaggaact gtccactggc aaagcacgtc
     3301 cgcaaacgcc ttcaccgacg agggattttt gacggggtgg cggcggtctg gtcatcggaa
     3361 gggcccaatt cggaaggcgc ggccggggga gaatctccgg acgccgacga ggaaccggca
     3421 cgcggcaggc agcgcatgcc gcttccaagc atggccccga tccccggaat cttcggattg
     3481 tcggcggcca actgggtcat tcgcggtatt cttgaaaaga caaaggtcgg ttaacatcga
     3541 caatccggac ttcacgccgg gagcatgaat gaacatactc ggtatctcgg ccttctatca
     3601 cgacagcgcg gcgtgcctgg tcatcgacgg ccgaatcgtg gctgccgccc aggaagagcg
     3661 cttcaccagg atccggcacg accccgcgtt tccttacaac gcggcgatgt actgcctgtc
     3721 gcgggccggc ctgcagccgg cggccatcga catggtcgtg ttttatgaaa agccgctgct
     3781 gaagcttgag cgcatcctgc agaacgccgt cgacgtggcg cccgccggat tcgtcccttt
     3841 ctgccgttcg atgccttcgt ggttttcgga aaagctgaac cttccacgca tcctgaaaaa
     3901 ggaacttggc tttcgcggac cgctgatgtt tgcgatacat caccaggccc atgcggcctc
     3961 ggcgttctac ccttccccgt tcaacgaggc agccatcctt accgtcgacg gcgtcggcga
     4021 atggagcacc ggctcgatcg gcgtcggtcg cggaaatgaa atcgagctga ggaaggaaac
     4081 caggtacccg cattcgcccg gcctgctcta ttcggccttc accgaatttc tcggcttcaa
     4141 ggtcaattcg gacgagtaca aggtcatggg cctggcaccc tacggcgagc cggtgttcgt
     4201 ggatgccatc ctcaatgaga tggttcacgt tgacgaagag ggcggattcg aactcaacat
     4261 ggactttttc cgcttccagt tcggttcgag aaccgccggc cccggattct cgcggcttgc
     4321 cggatgtcct ccgagaaatc ctgagtccag aattgaacag gttcacatgg atctggcccg
     4381 atcaatacag gccgtgattg aaatcatcat gctgcgccag gcacggttcg cccgcgaact
     4441 taccggaatg aacaacctgt gcatggcggg tggggtggcg ctgaactgcg tggcgaacgg
     4501 aaagatcctg aaggaacgga tattcgacga catctggatc cagcccgcgg cgggtgacgc
     4561 cggcggcgcg gtcggggcgg ccctgatcgg ctggtattcg accgagggtg ccagccgggt
     4621 cgtggatgag aacgattcga tgcagggcgc cctgctgggg ccggaattcg acgtcgccgg
     4681 catcgaggcc gccctgatcg aagccgggct gccctttgaa agatgcgggg acgacatcta
     4741 tcggcagatt gctgccggac ttgccgaagg acaggtgatc gcaaggtttt ccggacgaat
     4801 ggagttcggg ccgcgggccc ttggttccag aagcatcctc gccgacgccc ggaatccggc
     4861 gatgcagcga atcgtgaacg agaagatcaa attccgcgag ggcttccggc cgttcgcccc
     4921 ggccgtgctc cttgaccacg cggatgcctg gttcgacctt gatcgaagaa gcccgtacat
     4981 gctcctggtg gatcaggttg cctcaaggca gcttctggac agaccggcga tcgaggcgag
     5041 cggccttgat cggctgaaga tcccgatgtc aacgattccc gcggtgaccc acgttgacgg
     5101 ttccgccagg atccagacag tcgaaccatc cacgcagccg gacttcagcc gcatcatcga
     5161 cgctttctgg cagagaaccg gatgccccgt ggttcttaac accagcttca accttcgcgg
     5221 ccagccgatt gtgtgcaccc cttcggatgc ggttcagacg ttcctggcat gcgacattga
     5281 cgccctggcg gcgggccctt tcatggcacg caagccattc ggctggacca ggcgcccgct
     5341 gccgcgcccg acggctttca ggcgcccgcg gcaaaggggc gagcttcgca ggttcggcat
     5401 cgaaaacggt attttcttcg gtctgctggg aattcttgcc tggcttgcgc cggacagccc
     5461 gttcaggctg ggctccttcc tggcatgggg cgtttccgga ctggcactgc tggccggcct
     5521 ggctgccccg ggcatccttc gggccccgga ggatcggctc aacagggcgg ccgcccggat
     5581 gaacggcatc gccggcttca tcctgttcgg gctggtattc gtgctggttc tgaccccgac
     5641 cgcactgctg cgcaggatcg cgcgtgcaaa acgggaaccg gggggctggc gggatttctt
     5701 ccgcgaccat ggcggtcttg aaaacatgta ctgacactta ccggagcacg cgatgaacct
     5761 tgtcagggaa atatggggat gggcccgcag gcgcaagaag tactggctga tcccgctggt
     5821 gctcgccctt gttctggtgg gggcccttat agtcgtcgca tcggcaacca gcgtcagccc
     5881 cttcatctat ccgctgttct aggggttcat tcgacgtccg cgcccgtttc ggccggctcc
     5941 ttcggggcac cccgatccga atcttctgcg actatgcgaa ggaatatgtc cgccaggagc
     6001 cggtgtccct tttcgttgaa gtggcagcac gggtcgtacc tgatttccat gtagtcggct
     6061 tcggcctgca acagaagctg gtgaaggaac agcatcctga tgtcgtggga agacg
//
LOCUS       DAOPPA010000158         6078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14217_length_6078_cov_4.040345, whole genome shotgun sequence.
ACCESSION   DAOPPA010000158 DAOPPA010000000
VERSION     DAOPPA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14217_length_6078_cov_4.040345"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKH54_06185"
     CDS             <1..1394
                     /locus_tag="PKH54_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99512.1"
                     /translation="YRVVSGLLNVVTMVVITASVQAVSKLASQSGADLSRVRRTAMVG
                     QTLVFGPVFLAFIAFHGFIASNLLSDPGIARPLLVSGLIVLAYMYYAAIIGLLNGTCH
                     FGRQASMDILFSTLKTGLMIAAVVFSGSVALSYGAFAAAAWTVLIVAAFVSGRVFSGV
                     GRSSRGGVDLKEYLSYLLPLAGYALVLNLLLQADVIALKGFLGRLSGHGGSIGAADAA
                     SAAAGVYGAAKNVALLPYQAVISLAFVVFPIVSSASSRGDSERASRVVSGAFRLASLL
                     SWCAVAVFGSAPAETLGLLFGASYSSGAPGLVILLGAGAMLAFMHVGNAVLASSGRPI
                     VSVLGGVGAAVVQIVMLGILLPGASAADATWLAAVSTLCGSASGAIVAGILCGRVFGF
                     SGWVRSALSAAVSAVAALIAARLLRGACPWPVLPVVALAVFLAVLALTGGIGRADLGV
                     LRRVMSRRVRRAG"
     gene            complement(1378..2178)
                     /gene="truA"
                     /locus_tag="PKH54_06190"
     CDS             complement(1378..2178)
                     /gene="truA"
                     /locus_tag="PKH54_06190"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOC99513.1"
                     /translation="MTAIRCGPKMPGQTRNIRLLIQFDGTGFCGWQMQASDRTVQGVL
                     AEALNRLTGQPVRLYSSSRTDAGVHAVGMVVNFKLDTTLPLVAFHFGLNSLLPRDLQV
                     VMADEAPDNFNARFSARGKTYVYRIQPGHTRLPLEASRAWHVRGSLDVEAMQRAAAMM
                     LGRHDFNAFRSAQCDSDNPVRTVDQLTVEQGADGIVTITIRARAFLRNMVRIIAGTLV
                     EIGRGRKNPEWVIELLESGDRTKAGMTAPPQGLFLVKVHYPEDISQPA"
     gene            2236..4119
                     /locus_tag="PKH54_06195"
     CDS             2236..4119
                     /locus_tag="PKH54_06195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC99514.1"
                     /translation="MRLSALVAVMVALPSSWPALAASGAGAGNPGTPVVADDTVKPGG
                     GHWDPDGDELSRQVFSVFDFERIPPHAKPAFERLKPSDRPPHFHVFLVSWLVAVPLFV
                     VFVLWHFRRSRRSGASPPAASDAGVPDDLVPILVAIGLGAVVRLVAAALLPLHGSEWG
                     TVEHKPFSAIPDIAFSGYEVLTNPPLLNLVEHFVFLVCHHPFCFRLPIVLAGVWMIWS
                     SRTSARLMFGRRAGILAAFLVALHPGLVIWSATMRAYLPMAAFLVAAIPPAFRLARGD
                     DRDGDAVRLAIFCALAVWTHYIAWIWLALLGAVMLATLVLHPRRLLKPAISALFVLVS
                     FLPLVPFFFFDFGSKQGSMLPESWAVDTVALLSGMPHVSGIAVVAIIVAGRFWRSRPA
                     TALAAAISGYVGLCLATSPFIFWEVSYSVGVAAPVMILLAGCIDRMRFARVGTAVVLA
                     GFLAASIIVAREPSDSPVMARVSRPYLWRSASVEEFSRIISSGKQPASTRPATVLVTP
                     AFEAESFLYHLGPATASEAGVEPVIVRNFDRMSFSGPKGWYHVIGFERYWSWRLGQWP
                     ELSRFLRSSGSFWYARMQANCATGQGMTWSAWDCEWLSRHCRLVERNASDELYLCGPE
                     RGP"
     gene            4147..4851
                     /locus_tag="PKH54_06200"
     CDS             4147..4851
                     /locus_tag="PKH54_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99515.1"
                     /translation="MKSFSERIPAVVLAIFAVHVAASGASGAETCAERFIRARAGELG
                     TMKVDKVVPVETGVLASFSSPGKKGLVVVGMTVVGLDLVTSQTVFGRDNASDVGADES
                     KWLSGLFSKLHPDPEFRACPELIREQKPEAAAVSDVMKAWWKEQTELDNKLSGRTRLP
                     MIILVVSSVLVVLGLAFMIVVRRRRSAGGTEASAVAKTDSEASPVVASTVTEDIATDV
                     GDGSGVSADDDRGEVV"
     gene            complement(4858..5256)
                     /gene="speD"
                     /locus_tag="PKH54_06205"
     CDS             complement(4858..5256)
                     /gene="speD"
                     /locus_tag="PKH54_06205"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOC99516.1"
                     /translation="MNALGRHLVADFHDCTADLNDPVLILETLEKACEISGATIVSRT
                     RHHFSPYGVTAVFVIAESHLAVHTWPEYGYAAVDLFTCGSTVDTWKAFEHCKTAFGAG
                     RVEVKEIARGILPEASQAPAQGFASSGNAA"
     gene            complement(5458..>6078)
                     /locus_tag="PKH54_06210"
     CDS             complement(5458..>6078)
                     /locus_tag="PKH54_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99517.1"
                     /translation="FVTKGGSGSGGNPPKYFAVAMFGTEDGGATWNKAPELGKVEYDM
                     MSQSAPACATGRLFDADFIDRYTGFGVGEKGTVFRFQHKCTKQSDCEFGYFCSVEEAT
                     KLQCVPCSDIGLTTHPGSIDKCGGWEPPPEPDPDLGGRDAIEGTDSGSDSQGFSDVIS
                     GNDLRDETETSGSGCSGSARKSGTTGATLLLMLALIGIIQLRRRTT"
BASE COUNT          909 a         1847 c         1902 g         1420 t
ORIGIN      
        1 agtaccgggt ggtgtcgggc ctgctcaacg tcgtgacgat ggtcgtgatc acggcctcgg
       61 ttcaggcggt aagcaagctt gcgagtcagt ccggtgccga cctttcaagg gttcggcgca
      121 ccgccatggt cggccagacc ctggtattcg ggccggtatt ccttgccttc attgcgtttc
      181 acggattcat cgcgtcaaac ctgctgtccg atccgggaat tgcccggccg cttctcgttt
      241 cgggtctgat tgtattggcc tacatgtact acgcggccat catcggactc ctgaacggta
      301 cctgtcattt cgggcggcag gcctccatgg acatcctgtt ttcgacgctg aagacggggc
      361 tgatgattgc ggcggtcgtc ttttccgggt cggtggcgct ttcctacggt gccttcgccg
      421 cggccgcctg gacggtgctg attgtcgccg cgttcgtttc gggcagggtc ttttccggcg
      481 tcgggcgttc atcccgcggc ggggtcgacc tgaaggaata cctgtcgtat ctgctgccgc
      541 ttgccggcta cgcacttgtc ctgaatctgt tgcttcaggc cgacgtcata gcgctcaagg
      601 gcttcctggg gcgtctgtca ggccatggtg gttcgattgg cgccgccgac gcggcctccg
      661 ccgccgccgg cgtctatggc gccgcgaaga atgtggcgct gcttccatac caggcggtga
      721 tttcgctggc cttcgtcgtt tttccaatcg tttcctcggc gtcaagccgc ggtgacagcg
      781 aaagggcgtc ccgggtggtt tcgggcgcct tcaggctggc ttcgctcctg tcctggtgcg
      841 ctgtcgccgt tttcggctcg gcccccgcgg aaacgcttgg cctgcttttc ggtgcgtcat
      901 attcgtcagg cgcacccggt ctggtgattc tgctgggcgc tggggccatg ctggcgttca
      961 tgcatgtggg caacgcggtt cttgcgtctt ccgggcgccc gatcgtttcg gtgctcggtg
     1021 gcgtcggcgc tgccgtcgtc cagatagtca tgctggggat tctgcttccc ggcgcatctg
     1081 ccgccgacgc cacatggctt gccgctgtat cgacgttgtg cggttcggcg tccggggcca
     1141 ttgttgccgg catcctctgc ggccgcgtgt tcggcttttc cgggtgggtg cgctcggcct
     1201 tgtccgccgc ggtatcggca gtcgcggccc tgatcgccgc gaggctcctt cgcggggcat
     1261 gcccctggcc ggttcttccg gttgtcgcgc tggccgtatt cctggcggtt ctcgcattga
     1321 ccggcggcat cggtcgggcc gatctcggcg tccttagaag ggtgatgtcc aggcgggtca
     1381 ggcgggctgg ctgatgtctt ccgggtagtg caccttgacc aggaaaagtc cctgcggcgg
     1441 ggcggtcatg ccggccttcg tccgatcgcc cgactccagc agttcaatga cccattccgg
     1501 gtttttcctg ccgcgcccga tttcgaccag cgtgcccgca atgatacgta ccatgtttcg
     1561 aagaaacgcc cttgccctga tggtgattgt gacgatgccg tcggcgccct gctccaccgt
     1621 cagttgatcg accgtcctga ccgggttgtc cgaatcgcac tgggccgaac ggaacgcgtt
     1681 gaagtcgtgt cgccccagca tcatggccgc cgccctttgc atcgcttcga catccagcga
     1741 tccccggacg tgccaggccc ttgacgcctc cagcggaagc cgtgtgtgtc ccggttgtat
     1801 tcgatagacg taggtcttac cccttgcgga aaaccttgcg ttgaagttgt ccggggcctc
     1861 gtcggccatt accacctgaa ggtcgcgtgg aagcaggctg ttgaggccga aatggaacgc
     1921 aaccagggga agcgtggtat cgagcttgaa gttgacgacc attccgaccg cgtgaacccc
     1981 ggcatcggtt cttgacgagg aataaaggcg aaccggctgt ccggtcagtc tgttcagcgc
     2041 ctcggccagg acaccctgaa ccgtcctgtc ggaagcctgc atctgccatc cgcagaagcc
     2101 ggttccgtcg aactggataa ggagcctgat gttgcgggtc tgtccgggca tctttgggcc
     2161 gcacctgatt gcggtcatat gataacctca acaggtcgat tttgccttcc ttcggtgatt
     2221 ttccggggat tattgatgag actgtccgct ttggttgccg tgatggtcgc tctgccgtcg
     2281 tcctggccgg ctttggccgc gtcgggcgcg ggcgcaggca atcccggcac cccggtcgtg
     2341 gccgacgaca ccgtcaagcc aggaggcggc cattgggatc ccgacggtga cgaactttcc
     2401 cgccaggtct tttcagtctt tgatttcgaa cggattcccc cccacgcaaa gccggctttt
     2461 gaacgactga agccgtcgga caggcctccg catttccatg tttttctggt ttcctggctg
     2521 gttgccgtac ctttgttcgt ggtgttcgtc ctgtggcatt tccggaggtc gcgacgttcc
     2581 ggggcaagtc cgccggctgc gtccgatgcc ggcgttcccg acgatctcgt gccgatcctg
     2641 gtggccatcg ggcttggcgc cgtcgtccgg cttgtcgccg ccgcgcttct tccccttcac
     2701 ggatccgaat ggggaacggt cgaacacaag cctttttctg ccattcccga catcgccttt
     2761 tccggttacg aggtgttgac caacccgccg ctgctcaacc tggtcgagca cttcgtattc
     2821 ctggtctgtc accacccgtt ctgtttccgg cttccgattg tcctggccgg agtctggatg
     2881 atatggtctt cgcgaacctc ggcccgactg atgttcggcc gtcgcgcagg tattctggcc
     2941 gccttcctgg tcgcccttca tcccggcctg gtcatctggt cggccaccat gcgcgcatac
     3001 ctgccgatgg cggcttttct tgtggcggcc attcccccgg ccttcaggct ggcgcgcggc
     3061 gatgacaggg acggcgacgc ggtccggctt gccatcttct gcgcccttgc ggtctggacg
     3121 cattacatcg cgtggatctg gctggccctg ctgggcgccg tgatgctggc gacacttgtc
     3181 cttcatccgc gcaggctgtt gaagcccgcg atttcggcct tgttcgtcct ggtctccttc
     3241 cttccgctgg tgccgttctt cttctttgat ttcggctcga agcagggatc gatgctgccg
     3301 gaatcatggg cggtcgatac cgtggcgctg ctgtcgggca tgccccatgt ttccggcatt
     3361 gccgttgtcg ccatcattgt tgccggaagg ttctggcgca gccggccggc gacggcgctt
     3421 gccgcagcga tttccggata cgtcggcctc tgcctggcga caagtccatt catcttctgg
     3481 gaagtgtcgt actccgtggg cgtggcggcc ccggtcatga ttctgcttgc aggttgcatc
     3541 gaccgcatgc ggtttgcccg tgtcggcacg gccgtcgtgc tcgccggatt tcttgcagct
     3601 tcgataatcg ttgcccgcga accttccgac agccctgtca tggcgcgagt ttcgcgcccc
     3661 tacctctgga gatccgcatc ggtcgaagaa ttctcccgga tcatttcctc cgggaagcag
     3721 cccgcttcaa cgcggccggc caccgtactc gtcacgccgg ctttcgaggc ggaatcgttc
     3781 ctttatcacc tggggccggc gacggcttcg gaagcggggg tcgaacctgt catagtcagg
     3841 aacttcgatc gcatgtcgtt ttccggaccg aaaggctggt atcatgtcat cggattcgag
     3901 cggtactggt cctggcgcct gggccagtgg cccgaactat cccgattcct tcggtcatcc
     3961 ggcagcttct ggtacgcaag gatgcaggct aactgcgcga ccggccaggg gatgacatgg
     4021 tcggcctggg attgcgaatg gctttcgcga cactgccggc ttgttgaacg taacgcgtct
     4081 gatgaactat atttgtgcgg cccggaacgc gggccttgaa gtggcatgaa cgtggggttg
     4141 aacaacatga agtcattttc tgaaaggatt ccggcggttg tcctggccat tttcgcggtt
     4201 catgtcgccg cgtccggcgc cagcggcgcc gagacctgtg cagagcgctt catacgcgcc
     4261 cgggccggtg aactgggaac catgaaggtc gataaggtcg tgccggtcga gacgggagta
     4321 ctggcgtcat tttcctcccc tgggaaaaag gggctcgtgg tcgtcggcat gacggtcgtc
     4381 ggcctggatc tggtgacttc ccagacggtg ttcgggcggg ataacgcctc ggacgtcggc
     4441 gccgatgaat cgaagtggct ttccggactt ttttcgaagc ttcatccgga tccagaattt
     4501 cgcgcatgtc ccgaactgat aagggaacag aaacccgagg ccgcggcggt ttctgacgtg
     4561 atgaaggcct ggtggaagga acagaccgaa cttgacaaca agctgagcgg ccgcacgcgc
     4621 ctgccaatga taatcctggt ggtttcgtcc gttctggtgg tgcttgggct ggcgttcatg
     4681 atcgttgtgc ggcggcgcag gtcggccggc ggcaccgaag cgtcggccgt cgcaaaaacc
     4741 gattccgaag cctcgccggt cgtggcgtcg accgtcaccg aagacattgc gaccgatgtc
     4801 ggcgatggat ccggtgtatc ggcggacgat gatcggggcg aggtcgtata gccgctctca
     4861 ggctgcgtta ccgctggacg caaagccctg ggccggggcc tggcttgctt ccggaagaat
     4921 tccgcgcgcg atttccttga cttccaccct tccggcaccg aatgcagtct tgcagtgctc
     4981 gaacgccttc caggtatcga cggtgcttcc gcatgtgaac agatcgaccg cggcgtatcc
     5041 gtattccggc catgtgtgaa cggcaagatg tgactcggca atgacaaaga cggccgttac
     5101 gccatacggg ctgaagtggt gcctggtccg gctaacgatc gtggcgccgg aaatttcgca
     5161 agctttctcg agtgtttcaa ggatcagcac cggatcgtta aggtcagcgg tgcagtcatg
     5221 aaaatcagcc accagatgac gtccaagcgc gttcatttct ttgtcctccg atctgacacg
     5281 aggccggtgc gtcagcgttc cggccccagg ttcataagtc acctccggat tccgcgccgg
     5341 cggaatcgag tgccccgcga aggcggggac aagggcatac agtatgcggc aggataagtg
     5401 tcaatgtcaa cgatttctta atccaaatac ggggtttcgt ctgtttttca accgatttca
     5461 ggtcgtgcgg cggcgaagtt gaatgattcc aatcagtgcg agcatcaaga gcagggtggc
     5521 gcccgtcgtg ccggattttc gggcggaacc gctgcaacca ctacctgagg tttccgtttc
     5581 gtcgcgtaag tcgttgcctg aaatgacatc tgaaaatccc tgggagtcgg aaccgctgtc
     5641 ggtaccttcg atggcgtcgc gtcccccaag atcgggatca ggctccggag gcggttccca
     5701 tccgccgcac ttgtcgattg aaccggggtg tgtcgtaagg ccgatgtcgg aacacgggac
     5761 gcactgcagt ttggtcgctt cctccacgct gcagaaatag ccgaattcgc agtcggactg
     5821 tttggtgcat ttgtgctgga accggaacac ggttcccttc tcgccgaccc cgaatccggt
     5881 atagcggtcg atgaagtccg cgtcgaacag tcttccggtc gcgcaggccg gggcggactg
     5941 gctcatcatg tcatactcca ctttccccag ttccggggcc ttgttccagg tcgcgccccc
     6001 atcctcggtg ccgaacatcg ccacggcgaa gtactttggc gggtttccgc cgctgccgct
     6061 gccgcccttg gtgacaaa
//
LOCUS       DAOPPA010000159         6074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14229_length_6074_cov_4.535969, whole genome shotgun sequence.
ACCESSION   DAOPPA010000159 DAOPPA010000000
VERSION     DAOPPA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_6074_cov_4.535969"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKH54_06215"
     CDS             <1..200
                     /locus_tag="PKH54_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020328220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOC99518.1"
                     /translation="EARIIAVSDVFQALRQDRPYRAGMSIERALGLVDEMSLAGKLDP
                     DVVGLLHSNIDACDHAARAQA"
     gene            complement(270..1565)
                     /gene="purB"
                     /locus_tag="PKH54_06220"
     CDS             complement(270..1565)
                     /gene="purB"
                     /locus_tag="PKH54_06220"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOC99519.1"
                     /translation="MIDRYSTPRMREVWSSGWRWNTFLEVELAYLEALEEEGIAPGGD
                     ASRIRASVKMDPERIDELEGSLRHDVIAFLTHVEEQAGDVTRFVHFGLTSSDMLDTVS
                     AIQLNQACGYVREELSSLEKALMTQADRHRKTAMVGRTHGMHAEATSFGLVLLGWREM
                     VVRAMAGFDRAVEGIRFGKLAGAVGNLAFGDPEREARVLARFGLKPEPAATQIVARDR
                     HATLFSSMAVAGSVIDHIALNIRHLQRTEVGEAQEPFGGKQRGSSAMPHKKNPIGCEN
                     VCGQARLLRGYALAALEDVALWHERDISHSSVERVIGPDATATLEYIAKRLAGIVDGL
                     VVHEGRMGTNLESSGGVIHSEGLLLELVRAGLKRQQAYGLVQRAAMKYFNGEGTFRDN
                     LVSDPEVAALLTAEQIDGCFSLDHHLRWVDSIFDRRTRN"
     gene            complement(1636..2109)
                     /locus_tag="PKH54_06225"
     CDS             complement(1636..2109)
                     /locus_tag="PKH54_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99520.1"
                     /translation="MCASSDMALVVTDSSIPSGSGKKPSDVAFCIDKYELSSGDKPRG
                     GMSVGSASAACRKAGKKLCTAKQWQKACNNGAPAASNCNISGSMKKSGSSPKCVTKDG
                     VFDMIGNIGEVTTDREVRGGDVSGGGSGCDYSAGKHFQPKATDGARCCMDPEYKL"
     gene            complement(2144..3808)
                     /locus_tag="PKH54_06230"
     CDS             complement(2144..3808)
                     /locus_tag="PKH54_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC99521.1"
                     /translation="MEANTNLPGIGDRLANRYRIMFGRGAGPSGAVFKALDLENDLSV
                     ALKVLNPALFEGPFAAMNIARLSRACEIEDPNVVKVYDFIASEGRHFVTSLLVEGLSL
                     RAVLDLHDEGGEHFPVPKLKGFGERMLNGVRAFHRKGIVHGNIKPENFFVLPDRLALS
                     DPWHLVERKLRDGEQIPVSDYYRAPEQLNDPTTEMFESDLYAVGLILGELIAISPVKP
                     GLALSLQVPRLTKRFDELFTTATALDPDSRFDSVDAFAACMREAFSNVQKEGLWVRRM
                     HETGSFRAIRLPSQADRVTEVAPLDEVTVASLVPEPESRRYDDDDQDLVAIPEAAEPD
                     NQEIDEFQDDGRQAPIIPEADDDGGIEFVSESRSRPVAQDQGRGKGQHGKKGRNQSSQ
                     KKPEPREPMPRPIQAEQPRRAPEPAVAPPAPPPPEKFEPRPSTTRPVMIQPAKAETKV
                     PKSLLFVGIVIVVLLAVIAYLLTSRLHREIPAPTETVPSISAVAPAPQVPAKAPEPVP
                     APAAQPAPAAQPGAEQAPAAAPAPAPATRPPPPPPPPPPPAAPATA"
     gene            complement(3911..5851)
                     /locus_tag="PKH54_06235"
     CDS             complement(3911..5851)
                     /locus_tag="PKH54_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99522.1"
                     /translation="MNSGKFNVGTVVLLATMTAWAGCSGNGDPGLDEGSWQDTVDVSL
                     PDSNGGDTVDAVEPNDSTDTMDPDAVTDVPGTDGGEDADATPADTGFDATPDGLVATP
                     FQPPACGTPSTEWVGLIPGPGQEGYDAELEAKATRIERAHQIFVTAGLDCNKDISIDL
                     ENTADRQAIEDFLQESDSWDFKDYYGKEPLEVITTNHKVAGLYAGVGIAANAYRYGVL
                     RNQGYPCQEVERARQYLLASLDALHLAQELTGTPGVIARGFQRNDIPHNEVETVELFD
                     GDGNPLPAVKNNGTWRRDLSGLHPEYAWEDSVSRDMYIGWVKAQAAAWEVIRDDDSIP
                     AAFKDRLKADALLLAKELMVVRCHEEYAPDCYDLEIPDADGRTTFHGWLNPQNFDGQF
                     YLPGGENGFHAVMALGIVAAFAFITEDPEVVAWLNDDLIGTREFDRIVVEHGWEFVDM
                     GIKSNYSNYNMAYAGLWLCMRYLDNWPARLRLQNSIGSVFYDRPDSDRPAAEIGYSYY
                     DFIYATGDAGFHAWGRGWRDVDTDAVDRGMRTLKEFHDAPYWDFESILCPEAVCTPDE
                     PEVEVSECSPEEGLNLNVLGCVGRNGDLIAEQPIPMRLLGPSNYHWRSNPYRPNREGN
                     GSALLPSVDFRIAYWMGRWARR"
     gene            complement(6005..6074)
                     /locus_tag="PKH54_06240"
     tRNA            complement(6005..6074)
                     /locus_tag="PKH54_06240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6042..6044),aa:Gly,seq:ccc)
BASE COUNT         1053 a         1896 c         1909 g         1216 t
ORIGIN      
        1 tcgaggcaag gatcatcgcg gtcagcgacg tgttccaggc gttaaggcag gatcggccgt
       61 atcgcgcggg catgtcgatc gaacgcgcgc tcgggctggt cgatgaaatg tccctggcgg
      121 gaaaactgga tccggacgtt gtcgggctgc ttcactcaaa catcgatgcc tgcgaccatg
      181 ctgcccgcgc acaggcgtaa cgccggcggc ttgagagggg caggccctca acaagcgaca
      241 gtttccggcg tggatttcgg taaaagccgt cagttccgcg tgcggcgatc gaagatggaa
      301 tcgacccagc gcaggtgatg gtcaagcgag aagcatccgt caatctgttc ggccgtcagc
      361 agagcggcca cttcgggatc cgacaccagg ttgtcgcgga aagtcccttc gccgttgaag
      421 tacttcatcg ccgcccgctg gacaagcccg taagcctgct gacgcttcag gccggcgcgc
      481 accagttcca gcagcagccc ttccgagtgg atgacccccc cgctgctttc gagattggtc
      541 cccatccggc cttcgtgaac caccaggccg tcaacgatgc cggccagccg cttcgcgatg
      601 tattcaagcg ttgcggtcgc gtccggtccg attacgcgct cgacgcttga atggctgatg
      661 tcacgttcat gccacagcgc gacgtcttcc agcgcggcca gggcgtatcc acgcagcagc
      721 cttgcctggc cgcaaacatt ttcgcatccg atcgggttct tcttgtgcgg catggccgaa
      781 ctgccgcgct gcttgccgcc gaacggttcc tgggcctcgc ccacttcggt tctttgaagg
      841 tgcctgatgt tcagggcgat gtggtcgatt acgcttccgg caaccgccat cgatgaaaac
      901 agcgtcgcat gacggtcacg cgccacaatc tgcgtcgcgg caggttcagg tttcagtccg
      961 aacctggcca gcaccctggc ttcgcgttcc ggatccccga aggcaaggtt gccgaccgca
     1021 ccggccagct tcccgaaccg gatgccctca accgcgcgat cgaatccggc catggcccgc
     1081 acgaccatct cccgccagcc aagcagcacc agtccgaaag acgtggcctc ggcgtgcatg
     1141 ccgtgggtgc ggccgaccat tgcggtcttc cggtgtctgt cggcctgggt catcagcgcc
     1201 ttctcaaggg aagacagctc ttcgcggaca tacccgcagg cctggttcaa ctggattgcc
     1261 gaaacggtgt caagcatgtc cgaagaagtc agaccgaagt gcacgaagcg cgtgacgtcc
     1321 ccggcctgtt cctcgacgtg ggtcaggaag gctatgacgt cgtgacgcag cgatccttca
     1381 agctcgtcga tgcgttccgg atccatcttg accgaagccc tgatccttga agcgtcgccg
     1441 ccgggcgcga tgccttcttc ctccagcgct tccagatagg ccagttcgac ttcaagaaac
     1501 gtgttccagc gccatcctga agaccagacc tcgcgcatcc tcggtgtgga atacctgtca
     1561 atcacgggtt atctccatct tgctacggga tgaagcggcc aagtcggccg cgtgcggtcg
     1621 tattgtgcca gatttttaca gtttgtattc gggatccatg cagcagcgcg caccgtccgt
     1681 ggccttcggc tggaaatgct tgccggcgct gtagtcgcag ccggaaccgc cgccgctgac
     1741 gtcgccgcca cggacttcac gatcggtggt gacttcgccg atgttgccga tcatgtcgaa
     1801 aacgccgtcc ttcgtcacgc acttcgggct gcttccggac ttcttcatcg acccggagat
     1861 gttgcagttc gatgccgcgg gagcgccgtt gttgcacgcc ttctgccact gcttcgcggt
     1921 gcagagcttc ttgccggcct tcctgcaggc ggcagacgcc gaaccgacgc tcatgccgcc
     1981 acggggcttg tcgccggaag acagttcgta cttgtcgatg cagaatgcca cgtcggaagg
     2041 cttcttcccc gaaccggacg gaatcgaaga gtcggttacg accagagcca tgtcggacga
     2101 agcgcacatc accatctcgg ccattgcctt gaccttgggc ggctcaggcg gtggcggggg
     2161 cggcgggggg gggggggggg gggggggggg gggggggtcg ggtggccggg gccggggccg
     2221 gggccgccgc cggggcctgt tccgcgccgg gctgtgccgc gggggccggc tgggccgccg
     2281 gtgcaggtac cggttccggg gccttcgcag gaacctgcgg ggccggcgcg acggccgaga
     2341 tcgacggcac ggtttcggtc ggggccggga tctcgcggtg caggcggctt gtcagcagat
     2401 aggcgatgac cgccagcagg acgacgatca ctatgcccac gaaaagaagt gacttgggaa
     2461 cctttgtttc ggccttcgcc ggctggatca tcaccggcct tgtcgtggaa ggccgtggtt
     2521 cgaacttttc aggcgggggc ggtgctggag gcgcgaccgc gggctccggg gccctgcggg
     2581 gctgttccgc ctggatcggc ctgggcatcg gttcacgtgg ttccggtttc ttctgggatg
     2641 actggttgcg tcccttcttg ccgtgctgtc ccttgccgcg tccctgatcc tgggcaaccg
     2701 gcctggacct gctttcggat acgaactcga tgccgccgtc gtcgtcggct tcggggatga
     2761 tcggcgcctg ccggccgtcg tcctggaatt cgtcaatttc ctgattgtcg ggttccgcgg
     2821 cctccgggat tgcgaccaga tcctgatcgt cgtcgtcgta gcgtcttgat tcgggttcag
     2881 gaaccagcga ggcgacggtc acttcatcca ggggcgccac ctcagtgacc ctgtcggcct
     2941 gggatggaag gcggattgcg cggaaggaac cggtctcgtg catccttctg acccacaggc
     3001 cttccttctg gacgttcgag aacgcctcgc gcatgcacgc cgcgaaagca tcgacggaat
     3061 cgaaccgcga gtcgggatcc agcgcggtcg ccgtcgtgaa aagttcgtcg aagcgcttgg
     3121 taagccgcgg aacctgaagc gacagcgcca ggccgggctt gaccgggctg atggcgatca
     3181 gctcgcccag gatcagcccg accgcgtaca ggtcgctttc gaacatttcg gttgtcgggt
     3241 cgttgagctg ttccggcgcg cggtagtaat cggaaacagg aatctgctcg ccgtcgcgga
     3301 gcttccgttc gacaaggtgc cacggatcgg aaagagccag gcgatccgga agcacgaaga
     3361 agttttccgg cttgatgttt ccgtggacga tgcccttccg gtggaaggcg cggacgccgt
     3421 tgagcatcct ttcgccgaac cccttcagct tggggacggg gaagtgttcg ccgccttcgt
     3481 cgtgcagatc caggacggcc cgcagggaaa gtccctcgac aagaagactg gtcacgaaat
     3541 ggcgtccttc cgaggcgatg aagtcataaa ccttcaccac gttcggatct tcgatctcac
     3601 aggcacgcga aagccgcgcg atgttcattg ccgcgaacgg gccttcgaag agggccgggt
     3661 tcaacacctt cagggcgacc gaaaggtcgt tttccagatc cagagccttg aagaccgccc
     3721 cggacgggcc ggcaccccga ccgaacatga tcctgtaccg atttgcgagc ctgtccccga
     3781 taccgggaag attggtgtta gcttccatcc ttcactccag acgattgact tgcgcagtga
     3841 acagtcccga cgaggttatc agaaacagat tgttgaaaca acgcgggatt ttatgatccg
     3901 ggaaggatga tcatcgacgg gcccatcggc ccatccagta ggcgatcctg aaatcgacgg
     3961 aaggaagcag cgcggacccg ttgccctcgc ggttcggcct gtacgggttt gacctccagt
     4021 gataattgga cgggccaagc agcctcatcg gaatcggctg ttcggcgatc aggtcgccat
     4081 ttcgcccgac gcagccaagc acgttcaggt tcagcccttc ctcggggctg cattcggaca
     4141 cttccacttc cggttcgtcc ggcgtgcaca ccgcttcggg gcacaggatc gattcgaagt
     4201 cccagtacgg ggcgtcgtgg aattccttca gcgtccgcat cccgcggtcg acggcgtccg
     4261 tgtcgacgtc gcgccagccc cggccccatg cgtggaaccc ggcgtccccg gtcgcgtata
     4321 tgaaatcgta gtaactgtag ccaatctcgg ccgccggcct gtcggagtcg ggcctgtcgt
     4381 aaaacacgct gccgatggag ttctgaaggc gcagccgcgc cggccagttg tccaggtaac
     4441 gcatgcacag ccacagcccg gcgtaggcca tgttgtaatt ggaataattc gacttgatgc
     4501 ccatatcgac gaattcccag ccgtgctcca caacgatccg gtcgaattcc cttgtgccga
     4561 tcaggtcgtc gttaagccag gcgaccactt ccgggtcttc ggtgatgaac gcgaatgccg
     4621 ccacgattcc aagcgccatg accgcgtgga agccgttctc cccgccaggc aggtaaaact
     4681 gtccgtcgaa gttctgcgga ttcagccagc cgtggaaagt cgtccttccg tcggcgtccg
     4741 ggatttccag gtcatagcag tccggggcat attcctcgtg gcatcttacg accatcagtt
     4801 cttttgccag cagaagcgcg tccgccttca aacgatcctt gaacgccgcc gggatcgagt
     4861 cgtcatcgcg gatgacttcc cacgctgccg cctgcgcctt aacccagccg atgtacatgt
     4921 cgcggctgac cgagtcttcc catgcgtatt ccggatgcag tcccgacagg tcgcgacgcc
     4981 aggttccgtt gttcttcact gccggaagcg ggttgccgtc gccgtcgaaa agctcgaccg
     5041 tttcgacctc gttgtggggg atgtcgttgc gctggaaccc gcgcgcaata acgcctgggg
     5101 tgccggtgag ttcctgcgcc aggtgaagcg cgtccaggga cgcaagcagg tactgccgcg
     5161 cccgttcgac ttcctggcac ggatagccct ggttgcgcag cacgccgtaa cgataggcat
     5221 ttgcggcaat tccgacgccg gcgtaaagcc cggcgacctt gtggttcgtc gtgatgacct
     5281 caagcggctc ctttccgtaa taatccttga aatcccatga gtcggattcc tggaggaagt
     5341 cctcgatggc ctgcctgtcc gccgtgtttt caagatcgat gctgatgtcc ttgttgcagt
     5401 cgagtccggc cgtgacgaat atctggtggg cgcgttcaat ccttgtcgcc ttggcttcaa
     5461 gctccgcgtc gtacccttcc tggcccgggc cggggatcag tccgacccat tcggttgaag
     5521 gtgtgccgca ggcaggcggc tggaaaggag tcgccaccag tccgtccggc gtggcatcaa
     5581 acccggtgtc ggcaggtgtc gcgtcggcgt cttcgccccc gtccgtcccg ggaacatcgg
     5641 ttacggcgtc cggatccatc gtgtcggtcg aatcgttcgg ctcaaccgca tcgacggtgt
     5701 cgccaccgtt cgaatcgggc agcgagacat cgacggtgtc ctgccacgag ccttcatcaa
     5761 gaccgggatc cccgtttccg gagcatccgg cccaagcagt catggttgcc agcagcacga
     5821 ctgtcccgac gttgaatttc cctgagttca ttggaacctc gcatgtggtt tgcagcgtga
     5881 cgccgcccgg aagtccgggc ccgacaacgg tattcaacag ggattgccgc gggaagtcaa
     5941 cgcgcgggat ggccctggac gcgcgcccgg cgaggttgaa gtcgtggtca acgatgtcga
     6001 tttgagcggg atacgggagt cgaacccgcg acctacagct tgggaagctg tcactctacc
     6061 actgagttaa tccc
//
LOCUS       DAOPPA010000160         6058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14281_length_6058_cov_4.555389, whole genome shotgun sequence.
ACCESSION   DAOPPA010000160 DAOPPA010000000
VERSION     DAOPPA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14281_length_6058_cov_4.555389"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKH54_06245"
     CDS             <1..904
                     /locus_tag="PKH54_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99523.1"
                     /translation="LTKKVSALYRGVNDFRADLPNVLGKLGFEPETAAFLQQHIEVDP
                     ARGAGHAMGAGMRTDSAHLRTRVPESGMDYKGYNIALHELGHCVEQVFSLNRVDDTLL
                     DGVPNTAFTEAFAFVFQSRDLDVLGEANPDPEVMAMRSLDLLWSTVEIGSVGLLDMQM
                     WRWLYDHPDATPAQLRDAVVSMAKELWNKYFEPMFGIKDSPILAIYSHMVAYGLYLPD
                     YSVGFMIQAQIEAFLKGDDLATIKGRNLAREMERMCVQGRISPDVWMKGAVGSELSPD
                     AMLKAAEDALAVVERGQAGALEAR"
     gene            973..4500
                     /locus_tag="PKH54_06250"
     CDS             973..4500
                     /locus_tag="PKH54_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOC99524.1"
                     /translation="MRQGGTYRALLVAILLLPVLIMGDGRLRAGDGDAVTKAAAAALD
                     VPGPADNFGRGNAGSGGGEDDIRDVRDGTVLASSLDVDRRQPSPAVLVINSYHIGYEW
                     SDDIMVAIRDAFAATMPRANIYFEYLDSKRFPWDAERSRTWADELKRRYQGIRFDAII
                     VSDDIAYRFILQHHQSVFADVPVVFCGVNYFEPADIAGDPLITGVVQTVEFVGNVELI
                     RKILPSVRRVYAIVDKSLTGEAYRQAIQAEAGQIPDVDIQILDGRDISFDDLLVALAH
                     LENNSAALMLLFLEDKDGTYLPQHITYPRIAAASRVPVFQVTESGLGFGLGGGYIQTG
                     RSQGRTAALMAMDILRDVPVASLPVEVRANPEQRIDWNALSKYWGVSRKDFLSRTGAG
                     QPAQLPARPTRIEYKVCLSNEPVISRYNSIAGWFSGLAVELLEIVARRAGWHFEYVYV
                     EEAAMLQALNQGKCDLAMPSGGDELTHVKLDFTQQPLFSNWGAVYVREGSPVSGLRDL
                     RNMSVALVSDSAFATHFIDQAQDLGLNPGLVWADTYNEALTMLVEGTVDAAVVSRLHP
                     ISTRFAYRIRPSDILIYPTGQRIATLKGSNGEFLEAFDAYIPTLKADPSSGYMDVLSR
                     YTFKQKVAWVPAWIWLILGGFGIAIVFVVMLRGEVYRKTKELRDREENLTVMLDSIGD
                     AVIATDAGMVITRINPVACRLTGWSEFEALGRRLPMVFNVVDPVTRSPMNDWIQPVMA
                     GSSVNVGMDVAPQLLARTGAEMRVDQSASPIRDKDGKVVGAIVVFRDVTETIAREERR
                     RQSQKMEAIGQLAGGIAHDFNNILGGVVGIAEFLASRYREDPTILKYCGHIVHSGRRA
                     SQMTRRLLDFARLGKAESVPLSVNAIVASAVEILKGSIVRNIEIVTDLRASPDVIVGD
                     ASQIENMILNLGINSRDALPNGGRIQIATRTVDFGDAVVIGKFHLPAGRFVSVSVSDN
                     GVGMTADVRSRIFEPFFTTKETGKGTGLGLAAVMGIVEEHQGAIDVRSDISQGTEFTV
                     YFPLSGVQDTLEPELRSDEVRNYRGVVLVIDDEPIIRTMAEVLLSESGFDVITASGGR
                     EGIDIYKQRADNIDVVLLDFIMPGMDGLATLKELKAFDSSVRVVIASGFTSEHRRQNF
                     IDAGAVDAVGKPFSQQEICDVLARAASTR"
     gene            complement(4516..5268)
                     /locus_tag="PKH54_06255"
     CDS             complement(4516..5268)
                     /locus_tag="PKH54_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC99525.1"
                     /translation="MIDIDQSRQGSSVLAGITDRLVSSIEGIAFGPPVAFAYNPLVYA
                     RQSWDLYCARFGLSPRKVLLVGLNPGPFGMAQVGVPFGEISAVRDWMGIVAPVGRPRA
                     EHPARPILGFQCTRSEVSGRRLWGWAMDGWTTADRFFETFFVTNYCPLVFMESSGRNL
                     TPDKLPSSVRNALFSACDIAIRDTVTHFKPEFVMGMGTVTATRLREALDGFGVTVGQV
                     PHPSPANPAANRGWGELMDRSMAQIGISRPRG"
     gene            complement(5255..5626)
                     /locus_tag="PKH54_06260"
     CDS             complement(5255..5626)
                     /locus_tag="PKH54_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOC99526.1"
                     /translation="MVAVIVLLSLVVPILEVLLFVAVVNSAGIWAAVIPSAVTTVAGL
                     VILIRSFPKFMDSTFNNDKFQTFPTMQSYFEVTKILSGMLLLVPGFITDLAGLILLHP
                     KLRGVSFVILSRLMVRKNDRY"
     gene            5731..>6058
                     /locus_tag="PKH54_06265"
     CDS             5731..>6058
                     /locus_tag="PKH54_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99527.1"
                     /translation="MVISRSLFPILALSVLTSFPAVAGDVESPAAIDSSLVSTREPGQ
                     DSGQDAVLVNEGQPASSSDPDLSPDSHVLEQTRRQVSELAIELSRLRDEVDSLKAGRR
                     RGMKVKP"
BASE COUNT         1217 a         1712 c         1838 g         1291 t
ORIGIN      
        1 gctcacaaaa aaggtgtccg cgctttaccg tggcgtgaac gacttccgcg cggatctgcc
       61 gaatgtgctt ggcaagcttg gtttcgagcc cgaaaccgcc gccttcctgc agcagcacat
      121 cgaggtggat ccggcccgcg gggcagggca cgcgatgggc gccggcatgc ggacggattc
      181 ggcccacctt cgaaccaggg ttccagaatc cggaatggat tacaagggtt acaatattgc
      241 ccttcacgaa ctggggcact gcgtcgagca ggtgttttcg cttaaccgcg ttgacgacac
      301 gcttcttgac ggcgtgccga acaccgcctt taccgaagcc ttcgcctttg ttttccagtc
      361 ccgtgacctt gatgtgcttg gcgaggcaaa tccggatccg gaagtgatgg cgatgcgatc
      421 gctggatctg ctgtggtcca cggttgaaat cggcagcgtc ggcctgcttg acatgcagat
      481 gtggcgctgg ttgtacgacc atcccgacgc cacgccggcc cagcttcgcg acgcggtcgt
      541 ctcgatggcg aaggaactgt ggaacaagta cttcgaaccg atgttcggca tcaaggattc
      601 cccaatcctg gccatttaca gccatatggt cgcatacggg ctttatctgc ccgattattc
      661 cgtcggcttc atgattcagg cccagatcga ggcgttcctg aagggcgatg atcttgccac
      721 catcaagggg cgcaatctgg ctcgcgaaat ggaacgaatg tgcgtgcagg gcagaatcag
      781 tcccgacgtc tggatgaagg gcgccgtggg aagtgaactt tcgccggacg ccatgctcaa
      841 ggctgccgag gatgcgctgg ccgtcgttga aaggggccag gccggcgccc ttgaagccag
      901 atgaccaatc gtccccgggg cgtttcggat ttgtgttata atgcaccgac cttaaatcag
      961 gcgtattccc cgatgcggca aggcggcacc tacagggccc tgcttgtggc catcctgctt
     1021 cttcccgttc tgattatggg cgacggtcgc ctgcgggcgg gggatgggga tgcggttacc
     1081 aaggcagcgg cagccgccct ggatgttccg ggaccagccg acaattttgg ccgggggaat
     1141 gccggttccg gtggcgggga agatgatatc cgtgatgtgc gcgatggaac ggtccttgct
     1201 tcctcgcttg acgtcgatcg tcgccagccg tcgcccgcag tcctggtaat aaattcctat
     1261 cacatcggtt acgagtggtc cgacgacatc atggtggcga tccgcgatgc ttttgccgcc
     1321 accatgcccc gggcgaatat ctatttcgaa taccttgatt cgaaaaggtt tccctgggat
     1381 gccgagcgca gccggacctg ggccgacgaa cttaagcgca ggtaccaggg catccggttc
     1441 gatgccatca tcgtgtccga cgacatagcg tatcgtttta ttttgcaaca ccatcagtcg
     1501 gtttttgcgg atgttccggt cgtgttctgc ggcgtgaact attttgaacc tgccgatatc
     1561 gcgggcgatc cgctgattac tggtgttgtg cagaccgtcg agtttgtcgg caacgtcgaa
     1621 ctgattcgta agatcctgcc gtcagtgcgg cgggtatatg cgattgttga caagtccctg
     1681 actggcgagg cctatcgcca ggccatccag gccgaagccg gccagatccc cgatgtggat
     1741 atccagattc ttgacgggcg cgacatttcg ttcgacgacc tcctggtcgc tcttgcccat
     1801 ctggaaaaca attcggccgc gttgatgctg ctttttctgg aagacaagga cggaacctat
     1861 cttccccagc acatcacata cccaaggatt gcggccgcaa gccgggttcc ggtattccag
     1921 gtgaccgaga gcggcctggg attcgggctt ggcggcggat acatccagac cgggcgatcc
     1981 caggggcgga cggctgccct gatggccatg gacatcctgc gcgacgttcc cgtggcttcc
     2041 cttcctgtcg aggttcgggc caaccccgag cagaggatcg actggaacgc gctttccaag
     2101 tactggggcg tcagccgcaa ggattttctg agtcggaccg gggcagggca gccggcgcaa
     2161 cttccagccc gcccgaccag gattgaatac aaggtctgtc tctcgaacga gccggtaatt
     2221 tccaggtaca actcgattgc cggctggttc agcgggctgg cggtggaact cctggaaatt
     2281 gtcgcccgac gtgccggctg gcatttcgaa tacgtctatg tcgaagaagc cgcgatgctt
     2341 caggcgctga atcagggcaa gtgcgatctc gcgatgccgt cgggcgggga cgagttgaca
     2401 cacgtgaagc tcgacttcac gcaacaacct ctgttctcca actggggggc cgtttatgtc
     2461 cgcgaagggt cgcccgtttc cggattgcgt gacctgcgga acatgagcgt cgctctcgtt
     2521 tcggattccg ccttcgccac ccatttcatc gatcaggccc aggatcttgg cctgaatccg
     2581 ggcctggtgt gggcggacac gtacaacgag gccttgacga tgcttgtcga gggcaccgtg
     2641 gatgccgccg tcgtcagccg acttcatccg atttcgacaa ggttcgcgta ccgcatccgc
     2701 cccagcgata tcctgattta tccgacgggg cagcgcattg cgacgctgaa gggaagcaac
     2761 ggcgaattcc tggaggcgtt cgatgcctat atcccgacgt tgaaagccga tccgtccagc
     2821 ggatacatgg atgtcctttc ccgttatacc ttcaagcaga aggttgcctg ggttcccgcg
     2881 tggatatggc tgattcttgg cggcttcggc attgcaatcg tctttgtggt gatgctgcgg
     2941 ggcgaagtct atcgcaagac aaaggaactt cgcgaccgcg aggaaaacct gaccgtcatg
     3001 cttgattcaa tcggtgacgc ggtcattgcc acggacgccg gcatggtcat cacaagaata
     3061 aatcctgttg cttgcaggct gaccggctgg agtgaattcg aggcccttgg caggcgtctg
     3121 ccgatggtat tcaacgtggt cgatccggtc accaggagtc cgatgaacga ctggattcag
     3181 cctgtaatgg ccggttcgag cgtcaatgtg gggatggacg tcgcgcccca gcttctggcc
     3241 agaaccggtg ccgagatgag ggttgaccag agcgcctcgc cgatccggga caaggatgga
     3301 aaggtggtcg gggcgattgt tgttttcagg gacgtaaccg agacgattgc ccgcgaggaa
     3361 cgtcgccgtc aatcccagaa aatggaggcg attggtcaac ttgccggcgg aatcgcgcac
     3421 gatttcaaca atattcttgg cggcgtcgtg ggaattgcgg agttcctggc cagccggtac
     3481 cgggaggatc cgaccatcct gaagtactgc ggccatattg tccacagcgg ccggcgtgca
     3541 tcacagatga ccaggcgact gctggacttt gctcgcctgg gtaaggccga gtccgtgccc
     3601 ttgtcggtca atgccatcgt cgcgtccgcc gtcgagatac tgaaaggcag tattgtccgt
     3661 aacatcgaga ttgtgaccga tcttcgggcg tcgccggatg taatagtcgg ggatgcatcc
     3721 cagattgaaa acatgatcct gaatcttgga atcaactcac gcgacgcctt gccgaatggc
     3781 ggacgaattc agattgcgac caggaccgtt gattttggcg atgcggtggt gattgggaaa
     3841 tttcaccttc cggcaggccg gtttgtgtcg gtttcggtca gtgacaatgg cgtcggcatg
     3901 acggccgatg tcagatcccg catattcgag cctttcttca ccacgaagga aacgggaaaa
     3961 ggaaccgggt tggggctggc cgccgtcatg ggaatcgtcg aggaacacca gggcgccatt
     4021 gatgttcgaa gtgatatttc acaaggtacc gagttcacgg tatatttccc gctgtccggc
     4081 gtgcaggaca cattggagcc cgaattgcga agcgatgaag tcaggaacta tcgtggagtc
     4141 gtgctggtca ttgacgacga gccaataatc aggacgatgg cggaagtgct cttgtccgag
     4201 tctggtttcg acgtcatcac cgcgtcgggt ggaagggaag gaatcgacat ctacaagcag
     4261 cgcgccgaca acatcgacgt ggttctgctg gacttcatca tgcccgggat ggacggcctg
     4321 gccactttga aggaattgaa ggcgttcgat tcgtccgtcc gcgtcgtcat cgcatctgga
     4381 ttcaccagcg agcacaggcg ccagaatttc atagatgccg gcgcggtcga tgccgttgga
     4441 aagcccttca gccagcagga aatctgcgac gttcttgccc gggcggcttc aacccgttga
     4501 cgatgtcgcc gccgattatc cccgcggcct ggatatgccg atctgggcca tcgatcgatc
     4561 catcagttca ccccagcccc ggttggcggc cggattcgcc gggcttgggt ggggaacctg
     4621 gccgaccgtc acgccgaagc cgtcaagtgc ttcccgaagt cttgttgcgg tcaccgtacc
     4681 cattcccatc acaaattccg gtttgaagtg cgtcacggta tcgcggattg cgatgtcgca
     4741 cgccgaaaaa agcgcattcc tgactgacga cgggagcttg tcgggtgtaa ggttcctgcc
     4801 ggacgactcc atgaaaacca gagggcagta gttcgtgacg aagaaggtct cgaaaaatct
     4861 gtcggcggtc gtccatccgt ccatggccca tccccaaagg cgtctgccac tgacttcgct
     4921 gcgggtgcat tggaatccaa gaattggcct ggctggatgt tccgcgcgcg gccgaccaac
     4981 cggggccacg attcccatcc agtccctgac cgccgaaatc tcgccgaacg gaacgcccac
     5041 ctgtgccatt ccgaatgggc cgggattaag tccgaccagc agcacctttc tcgggctcag
     5101 gccaaagcgt gcgcagtaaa gatcccatga ctgcctggcg taaaccagcg ggttgtaagc
     5161 gaaggccacc ggtggcccga aagcgattcc ctcgatcgag gaaacaagcc tgtccgtgat
     5221 tccggcaagc accgaggaac cttgtcgcga ctggtcaata tcgatcattc tttcgcacca
     5281 tcaagcggga aaggatcacg aacgatacgc cccgcagctt tggatgaagc aggatcaggc
     5341 cggccagatc cgtaatgaag cccggaacaa gcagaagcat gcctgaaagg atcttcgtga
     5401 cttcgaagta tgactgcatg gttggaaatg tctggaactt gtcgttgttg aatgtactgt
     5461 ccatgaattt cggaaacgat cggataagta tgaccaggcc ggcgaccgtc gtcaccgcgg
     5521 acggtatgac cgcggcccag atcccggcgg agttgacgac cgccacgaac agcaggactt
     5581 caagaatcgg cacgacgagc gaaagcagga cgattacggc caccattgga acttcccgga
     5641 tgcgcgactg cgcgatcata caacggtgta ttggaaaacg gggcaatcca tgcaagaatc
     5701 cagcctggtt gccggtttat ggggtttgtg ttggtcattt cgcggtcgct ttttccaatt
     5761 ctggcccttt cggttctcac atcgtttccg gcggtggccg gcgacgtcga atcacccgca
     5821 gcaatcgatt cgagcctggt ttcgacccgt gagccaggcc aggattccgg ccaggacgcc
     5881 gttctggtta acgaaggcca accggcctcc agctccgacc ccgacctgtc gcccgattcc
     5941 catgtccttg aacagacgcg gcggcaggtt tccgaactgg cgatcgaact ttcgcgcctt
     6001 cgcgacgagg ttgattcttt gaaggccggc cgtcgtcggg gcatgaaggt caaaccca
//
LOCUS       DAOPPA010000161         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14296_length_6053_cov_3.522174, whole genome shotgun sequence.
ACCESSION   DAOPPA010000161 DAOPPA010000000
VERSION     DAOPPA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14296_length_6053_cov_3.522174"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKH54_06270"
     CDS             complement(<1..266)
                     /locus_tag="PKH54_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOC99528.1"
                     /translation="MGTADFAVPSLRAIVDAGCVVPLVVTRPDKPVGRGLEMSMTPVK
                     TAAMELGLEVFQPAGLRDTAVQDRLRACDADFFVVAAYGRILPSA"
     gene            complement(296..811)
                     /gene="def"
                     /locus_tag="PKH54_06275"
     CDS             complement(296..811)
                     /gene="def"
                     /locus_tag="PKH54_06275"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC99529.1"
                     /translation="MAILKIVKWPEEILSRKTVQVESVDDAVRRLLDDMAETMYASNG
                     IGLAAPQVGSSLRVAVIDVPADGDNPATGLVCLVNPLVVSGDGTVSIDEGCLSFPGIE
                     VPVKRSGHVVVEYLDRNGVPSRIEATDLAAICLQHEIDHLDGITMIDRVGPIRRKLAI
                     RDYQKAMHKVL"
     gene            complement(839..1171)
                     /locus_tag="PKH54_06280"
     CDS             complement(839..1171)
                     /locus_tag="PKH54_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99530.1"
                     /translation="MEAELKRRRNPPGRPDRGASSAGDMVAQLWDRARAGTYFDCLGL
                     APDAGTGDVREAWLRMSEAMAGLRQSVAGDPQALEILARVEKVASDGFEVLSDPDLRL
                     AYVKALDK"
     gene            complement(1156..2598)
                     /gene="cysS"
                     /locus_tag="PKH54_06285"
     CDS             complement(1156..2598)
                     /gene="cysS"
                     /locus_tag="PKH54_06285"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOC99531.1"
                     /translation="MGLKLFNTITRSKVDFEPIEDGKVGMYVCGVTVYDRCHIGHARA
                     YVAFDTIQKYLRYSGYQVRYVRNFTDVDDKIISRANERGISVNELTDTFIEAFNRDMA
                     ELGISPADVEPRVTGHIPEIIETVKTIIDRGHAYVSGGDVYYDIQSYPGYLGLSGRRL
                     DDMQAGASDRVDSTDAKRHPMDFALWKAAKPGEPAWDSPWGPGRPGWHIECSTMSAKY
                     LGDVFDIHGGGADLVFPHHENERAQSWAVSGKDFVRYWMHNGFVQVDKEKMSKSLGNF
                     FTIRDVIARFHPESLRYFLMTTHYRSPINFTDSAIDEAENRVEYLYEGVLAARKWLSA
                     HPADAVAADLAAYRERFVQIMDDDFNTAAALGLLAEEARGLNEALATKGKSPEKSARI
                     ATLLAAIMDQGGVLGLCVRDPEETLARIQAGKLARSGLDREAIIALVDERNRARADKD
                     FARADDARNRLLQMGVSVMDTPEGTRWKLN"
     gene            2715..4088
                     /locus_tag="PKH54_06290"
     CDS             2715..4088
                     /locus_tag="PKH54_06290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC99532.1"
                     /translation="MNASKNAMLAGGAIFVIAAAIFVETIEPEPLPVETTNTIELRQA
                     LFLAGSLPSGNSGILPADQPAPYDSIATGMLEELAGRSFMTDSDGLTAAAASVALGAR
                     HTAMLFAAPHAGSVPGAMSIVRWAADPSFTPSMTDLQAIRAMNCRQWARDRAVTRIFK
                     PQFPNLPEETREGNRSAILSAAATVAMLAGISVLIWGIVCWSRWRRRQFAEVLGARER
                     EPAAESPLTGLFSTYIWFTAWFLAVGLLLPQWLQFFNLSAPVMSIIVYSVTGGGGLII
                     AAWFGRSPSENDWKSALGFTSTLPPPPAAFPRKGSLRAALDGYSMVWPIVIVATLIGS
                     FIGDTGSGLDNPVSLFLITEKDISGRVLLVLSAVILAPIFEEPVFRGLFYRRLRVILS
                     PYGAAAASGFIFAAAHFPASGFLQLWAIGFTLGLAYEYSGSLKSSMIVHGLWNLGTSI
                     SILSLFN"
     gene            4198..5949
                     /locus_tag="PKH54_06295"
     CDS             4198..5949
                     /locus_tag="PKH54_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC99533.1"
                     /translation="MDHVEINDRPWLKFYEPSVPTSFPETCTLCGDGDDGPASSLWEQ
                     FERTAHAWPDSIAIVFEGTSLTYRQLKALTDQFAVALCRLGVKHGDRVVINLPNLPQT
                     VISYYAVHRIGAIAVMANPMYVTPELAHTFADSGAAAVITLDAYWVHKIRRIKTSTAL
                     RAVIVTSIADYLPFPKKQIFPIIGKRKGVWEKVRREQGVYRFLDMVEPEEKWPDPAEL
                     PANPGGPDDPACLQYTGGTTGISKGAVLTHRNLISNVHQICVWFPGLQPGKEIFMCAL
                     PFFHVFGMTVCLNTAISIGACMVLVTDPRNLKGLIGKIEKLRPTLFPAVPALFEGINR
                     FPGIDRRDLSSIRGCFSGSAPLSLEVMSRFEALTGARITEGFGLTESSPVTHVNPLFG
                     LRKEGSIGVPMPGTDARVVDLETGLVDLPVGEPGELIIRGPQVMKEYWNQPEETAIAL
                     RDGWLYTGDIATMDQDGYFFIVGRKKEMIIAGGYNIYPREIDEVLYMHPEIQEAVVVG
                     MPDQHRGETVWAFVVLKPGATLTAESVERWCAKYLARYKIPRRVIFRDSLPRTTVGKV
                     LRRELKEIGLEMMKSEQ"
BASE COUNT         1199 a         1921 c         1719 g         1214 t
ORIGIN      
        1 gctgacggaa gaatccggcc gtacgcggcc acgacgaaaa aatccgcatc gcacgcacga
       61 agacgatcct gaacggcagt atcgcgcagc cctgcaggct ggaatacttc caggccaagc
      121 tccatggccg ccgtcttgac cggggtcatg gacatctcca agccacgacc gacgggcttg
      181 tcgggcctcg tcaccacaag gggcacgacg catccggcat ccacgatggc gcgcagggac
      241 ggcaccgcga agtcggcggt acccatgaag accactctca gaccgtggat tggattcata
      301 aaaccttatg catggccttc tggtaatcgc gaatcgccag cttgcgccgg attggaccga
      361 cgcggtcaat catggtgatg ccgtcaaggt ggtcaatctc gtgctgcagg catatcgcgg
      421 caaggtcggt tgcctcgatc ctcgacggca cgccgttcct gtccagatac tcgaccacga
      481 cgtgaccgct gcgcttgact ggaacctcta taccgggaaa agaaagacac ccttcatcga
      541 tcgagaccgt tccatccccc gaaacaacaa gcggattgac caggcagacc aggccggtcg
      601 ccggattgtc gccatcggcc gggacatcta tgacggcaac gcgcagggaa cttccaacct
      661 gcggcgcggc aagtccgatg ccgttgctgg cgtacatggt ctcggccatg tcatccagaa
      721 ggcgccgaac cgcgtcatcg acagattcga cctggacggt cttgcgggaa agaatttcct
      781 ctggccattt gactatcttc agaattgcca tacgccacct ccatcccggc cgcggccatc
      841 acttgtcaag cgccttgacg tacgcaaggc gcaggtcggg atcggaaagc acctcgaatc
      901 catcggaagc cactttttcc acgcgcgcca gaatttccag cgcctgggga tcaccggcca
      961 ccgactggcg caggccggcc attgcctcgg acatcctcaa ccacgcctcg cggacgtcgc
     1021 cggtgccggc atcgggcgca aggcccagac agtcgaaata cgtcccggcc ctggcgcgat
     1081 cccacagttg agccaccatg tcgccagcgg atgacgcacc acgatccgga cggcccggcg
     1141 ggttgcgccg acgcttcagt tcagcttcca cctggtaccc tccggggtgt ccatgaccga
     1201 cacgcccatc tgaagaagcc tgttcctggc gtcgtcggca cgcgcgaagt ccttgtccgc
     1261 ccttgcacgg ttgcgttcat cgacaagggc gataatcgcc tcgcgatcaa gccccgatct
     1321 ggcaagtttt cccgcctgaa tccgggccag ggtctcctcg ggatccctta cgcacaatcc
     1381 cagcacgccg ccctgatcca ttatggccgc aagcagcgtc gcaatccttg ccgacttctc
     1441 gggggacttg cccttggtgg ccagggcctc gttcagcccg cgcgcctctt cggccagcag
     1501 gcccagggcc gccgccgtgt tgaaatcgtc gtccattatt tgcacgaacc gctcccgata
     1561 ggctgcaagg tcggcggcaa cggcatccgc cggatgcgcc gacagccact tcctggcggc
     1621 gagcacgcct tcgtacaggt attcgacgcg gttctcggcc tcgtcaatcg cggaatccgt
     1681 gaaattgatg ggactgcgat aatgcgtcgt catcaggaag taacgaaggg actcggggtg
     1741 gaaacgcgcg atgacgtccc ggatggtgaa aaagttaccg agcgacttcg acatcttttc
     1801 cttgtcaacc tggacgaagc cgttgtgcat ccagtatctg acgaagtcct ttccggaaac
     1861 cgcccaggac tgggcgcgct cgttctcatg gtgtggaaag accagatccg ccccgccgcc
     1921 gtggatgtcg aacacgtcgc caaggtactt ggcgctcatc gtcgagcatt cgatgtgcca
     1981 gcctggcctt cccgggcccc acgggctgtc ccacgccggt tcgcccggct tcgccgcctt
     2041 ccacagggcg aagtccatcg ggtgcctttt cgcgtcggtc gaatcgaccc tgtcgctcgc
     2101 ccccgcctgc atgtcgtcca gccggcggcc ggagaggccc aggtatcccg gataggactg
     2161 gatgtcgtaa tagacgtccc cgcccgacac atacgcgtga ccgcgatcaa ttatggtctt
     2221 taccgtctcg atgatttccg ggatatgccc ggtcaccctt ggttcaacgt cggccggcga
     2281 aatccccagt tcggccatgt cccggttgaa cgcctcgata aaggtgtccg tcagttcgtt
     2341 gacggaaatt cccctttcgt tcgcgcgact tatgattttg tcatcaacat cggtgaaatt
     2401 gcgtacgtac ctgacctggt atccggaata ccgcaggtac ttctggatgg tgtcaaaggc
     2461 tacataagcc cgcgcgtgtc cgatgtggca cctgtcataa accgtgacgc cgcagacgta
     2521 cattccgacc ttgccatcct cgatcggctc gaaatcgacc ttggaacgcg tgattgtatt
     2581 aaaaagcttg agacccactg gcatcctccg ggaagaacac aaagacttcc gtgtgaaaat
     2641 aggttccaat ccgatcgctg tcaatcttcg ttcatgattg tcgatcaagg gcgactatct
     2701 gctatagtcc gcgcatgaac gcttcaaaga acgccatgct ggccggcggg gccattttcg
     2761 tgatcgcggc ggcgattttc gtcgaaacga tcgagcccga gccgcttccc gtggagacga
     2821 ccaacaccat cgaactgcgg caggcccttt tcctggccgg atctcttccg tcggggaatt
     2881 ccggcatcct tccggcagac cagccggcgc cgtacgactc gattgccacc ggcatgcttg
     2941 aagaactggc cggccggagc ttcatgaccg attcggacgg cctgaccgcc gcggcagctt
     3001 cggtggccct gggagcaagg cacactgcca tgcttttcgc ggcgccgcat gccggttccg
     3061 tgcctggcgc catgtcaatc gtccgatggg cggcggatcc gtcatttaca ccgagcatga
     3121 ccgatcttca ggcaatccgg gccatgaact gccgacagtg ggccagggat cgtgccgtga
     3181 ccaggatttt caaaccgcag ttcccgaatc ttcccgagga gacccgcgaa gggaaccggt
     3241 cggcgattct ttcggccgcc gcgaccgtgg ccatgctggc cggaatttcc gtgctgatct
     3301 ggggcatcgt ctgctggtcc agatggcggc gaaggcagtt tgccgaagtc cttggcgcgc
     3361 gtgaacgcga accggcggcc gaaagcccgc tgacgggact gttttccacg tatatctggt
     3421 tcactgcctg gttcctggcc gtcggactcc tgttgcccca atggcttcag ttcttcaacc
     3481 tttccgcccc agtgatgtcc atcatcgtct attcggtgac cggcggcggt ggactgatca
     3541 tcgcggcctg gttcggaaga agcccgtccg agaacgactg gaagtcggca ctcggcttca
     3601 cttcaacgct gccgcctccc ccggccgcct tcccgcgaaa gggttccttg agggccgccc
     3661 tggacggcta ttccatggta tggccgatcg taatcgtcgc caccctgatc ggctcattca
     3721 tcggcgacac cggaagcggc ctggacaacc ccgtttccct tttcctgatc accgagaagg
     3781 acatttccgg aagggtgctt cttgtccttt cggccgtaat cctggccccg atcttcgagg
     3841 aaccggtgtt ccggggactt ttttaccgca ggcttcgcgt catcctctcc ccgtacggcg
     3901 cggccgccgc cagcggcttc atcttcgccg ccgcccactt cccggcatcc ggattccttc
     3961 agttgtgggc cattggcttc acccttggac ttgcatacga gtattccgga agcctgaagt
     4021 catcgatgat agtccacgga ttgtggaacc tggggacgtc cataagcatc ctttcccttt
     4081 tcaactaggc gcatcagcaa gggaacgcct gataataaca agatttcctt ccgacatctt
     4141 tgaccgcgga cacggctgga atgcaaaatt caattaactc ctgcacggac atattcgatg
     4201 gatcatgtcg aaatcaacga ccgaccctgg ctgaagtttt acgaaccatc ggttccgacc
     4261 agttttccgg agacttgcac gctgtgcggg gatggcgatg acggcccggc gtcttcactg
     4321 tgggaacagt ttgaacgtac cgcgcacgcc tggcccgaca gcatcgcaat cgtattcgag
     4381 ggaacaagcc tgacctaccg acagttgaag gccttgaccg accagttcgc ggtcgcgctg
     4441 tgcaggctgg gggtcaagca tggcgaccgg gtcgtgatta acctgcccaa cctgccccag
     4501 acggtaatat cgtactacgc ggttcacagg attggcgcaa tcgccgtgat ggccaacccg
     4561 atgtatgtca cgccggagct tgcccacacc ttcgccgact ccggggccgc ggccgtcatc
     4621 accctcgacg cctactgggt tcacaagatc cgtcgcatca agacttcaac cgcccttcgc
     4681 gccgtcatcg tcacgagcat cgccgattac cttccgttcc ccaaaaagca gatcttcccc
     4741 attatcggaa agcgtaaggg cgtttgggaa aaggttcgcc gggaacaggg tgtttacaga
     4801 ttcctcgaca tggttgaacc ggaagagaag tggccggatc ccgccgaact gccggccaac
     4861 cccggcgggc cagacgaccc ggcatgcctg cagtacaccg gcggcacgac cggcatatcc
     4921 aagggcgcgg tgttgaccca ccggaacctg atatcgaacg tccaccagat ctgcgtgtgg
     4981 tttccaggcc ttcagccggg aaaggaaatc ttcatgtgcg cgcttccgtt cttccacgtc
     5041 ttcggtatga cggtatgcct gaacacggcc atatcgatcg gcgcctgcat ggtgctggtt
     5101 accgacccga ggaacctgaa ggggctcatc ggcaagatcg aaaaactgcg cccgacactg
     5161 ttcccggcgg ttcctgccct tttcgaaggc atcaacaggt tccccggcat cgacaggcgc
     5221 gacctttcgt ccatacgagg atgtttctcg ggttcggccc cgctttccct tgaagtaatg
     5281 tccaggttcg aagccctgac cggggcaagg attactgaag gtttcggtct taccgaatct
     5341 tcgccggtca cacacgtcaa cccgctgttc gggcttagaa aggaaggatc catcggggtt
     5401 ccgatgcccg ggacggacgc aagggtcgtt gatcttgaaa ccggccttgt cgatttgccg
     5461 gtcggtgaac cgggcgagtt gatcatccgg ggaccgcagg tcatgaagga atactggaac
     5521 cagcctgagg aaacggccat cgccctgcgg gacggatggc tgtacaccgg cgatatcgcc
     5581 accatggatc aggacggcta tttcttcatt gtcggccgca aaaaggaaat gatcatcgcc
     5641 ggcgggtaca acatctatcc gcgtgaaatc gacgaagttc tgtacatgca cccggaaatc
     5701 caggaggccg tcgtcgtcgg aatgccggat caacaccgtg gcgaaaccgt ctgggccttc
     5761 gtggttctga agcccggcgc gacgctgacc gccgaatccg tcgagcgctg gtgcgccaag
     5821 tacctggcca ggtacaaaat tccaagacgc gtcatattcc gggactcgct tccaaggacg
     5881 actgtcggga aggtgcttcg acgtgaactg aaggaaatcg gactggaaat gatgaagtcg
     5941 gaacagtgac cgcggaatcg ctagtttccg gcggaagcac gccccagcct tgaaagcgcg
     6001 accagcacgc aggcatccct tacaacggtc tcgaaccggc cttccgcgaa ccg
//
LOCUS       DAOPPA010000162         6042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14336_length_6042_cov_4.216970, whole genome shotgun sequence.
ACCESSION   DAOPPA010000162 DAOPPA010000000
VERSION     DAOPPA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14336_length_6042_cov_4.216970"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKH54_06300"
     CDS             <1..341
                     /locus_tag="PKH54_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99534.1"
                     /translation="VGSLEMMEPEEFIFSDMTCVLQDAKKSVNDEYFFKVLKDPLSVR
                     WGYNEQWMYHCTGQTEYDLAVILHFFTAQGTACSQIDDVGISDEKAPQGEYTTSCAGT
                     GEVTAEWDFR"
     gene            complement(367..1347)
                     /locus_tag="PKH54_06305"
     CDS             complement(367..1347)
                     /locus_tag="PKH54_06305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC99535.1"
                     /translation="MGAINPDTAVSNSFDYVPVRPIPGFILDPAACSARDHGLAASGT
                     SRVVLVNAAGRASEDGDGGLREVGRTVLCRTDTADFDEAEIRHVVHDRGQLYLDVSGK
                     PSIDDFATDVRQLVLARECMRCPDFVQCCCCYLPAETSFFDQDQRWLADMMKSLGGRV
                     LDVGLGNVPYMGFMPIPPDGEHIEYHGVDPDPDAGKTIAGVTVHQIGIEDFAGFDGYF
                     DHVLSLRSLNHFLDVGAALESVHRCLKPGGQAVFMESLALPLVRSRHKALTSHQAAAG
                     GFQHLRNWGSSQLLEVVERMNRFGRGFRVTFHRPIGPDTCDQWIVVMTAA"
     gene            complement(1353..2306)
                     /locus_tag="PKH54_06310"
     CDS             complement(1353..2306)
                     /locus_tag="PKH54_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99536.1"
                     /translation="MSDTGRDAGPLEGAGGRGVDYGDVMVDLIMGYECNVKCDYCSVS
                     DAMRPVNMTTAEALSELAAARAMGMHKVAFGGGEPTIRRDLLPIARWARDRGFDWIKV
                     SSNGLMYAYQDYASRAIDAGVTDFHISVMGHEPRMYAATMGDAKWFDMVVRGVDNLVA
                     AGHVPILDMIIKADTWRTLTETVAFWAGHGVRTFPLWLVSLTDRNADKPDSLPRVRDI
                     FPELARVFEYSRAAGLNVYSRHIPRCMIPGYHDFVHDLRQDRVYIVTPGSRFFLWESD
                     ISANTYLPACEGCRYVRAECQGARRDYLARFGGDEIVPIKG"
     gene            complement(2299..4644)
                     /locus_tag="PKH54_06315"
     CDS             complement(2299..4644)
                     /locus_tag="PKH54_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99537.1"
                     /translation="MRQWKVAIFASGCRMPSRSMLFIVPPMVLEGDFIDYPWAADLGA
                     LCSAARVAAAGWRVTVFDALARPTSGWHTEGGLQVAGVPFGRVAVGAVELIDGLSPDI
                     VVVANHPFLWASRVPAAAMIRDVAASFPGVPIVLADCGVGGMMYVERPVDDVFSAFPG
                     ISCYLSYSGESHFADPDALLDAVLSGARHIPSPAVSWDAIPPQPAPMWDSIDITSRHA
                     FNVRFFGESGRPNPFAVGAGSLPMLASAGCAHDCVFCTTNPGRDNHGSGAYRTVPLAA
                     LADQLYLMKRAYSCRHVLFLDDAANLRGDFADLLKILADLGLDYDFPNGLRADRLDDA
                     AISAMKGRVTTLSVSCETADPAALRDRVCKRLDPADIERVAESAARHGVPLMVHFIIG
                     FPWETLADVRRTLDFARILFETHGAVPAVQFATPIPGARMERQLREAGIPFLAADGES
                     FQHRPGFVPPGMLPGELEDSMLAFNRRIEASRPSKVIINVTYDCINACQFCAVSNRVR
                     RSIPTARIIEMLDEHRSRGIDNVDFDGGEPMLHPGILDVVGHAAAVGYRQINVTSNGR
                     KLADRELASALVKSGVTSILISLHGDTAQVHDAITGKPGSFDETVAGIDNVMRLVGQS
                     GRQIDFGVNVTVCATNMGHMVGLARMMADRGVPKMNFQFLTPFGAASAGLVPDPIQAA
                     RQVQAAIDAVADRMKIYVVNSQMCLFEPAYQKYLLNDLQKLGRTMVFVWEEEVNLFRY
                     LAERRVRRAQCASCPHFLVCDGFYEFPESGELAMQDGGRHE"
     gene            4756..5580
                     /locus_tag="PKH54_06320"
     CDS             4756..5580
                     /locus_tag="PKH54_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99538.1"
                     /translation="MKTKGGYPMRDLWRSPLSDHFINDIELFGLAAGLRPVAKKEGRD
                     ADEILKISGPMRDIGFLVVRNPETGCVYVSRESDRIDRLMQLENGEFGGGNPEARMQV
                     IREIGICLGYPDCCATAFARHRMQDDGYVMGCFIECEKAVAHGNIVRNIPWQLNFLPP
                     MVSPVFFYPCHARCGQALALADRYIAEMDAVRPGTAAAVKRQLRGVVLATGRWDFVMF
                     DGTIEGSSINYDSWRPPVSFHPVPLPTAAFSVFMDALPESGRVIPDDPRVIVLEYV"
     gene            complement(5596..>6042)
                     /locus_tag="PKH54_06325"
     CDS             complement(5596..>6042)
                     /locus_tag="PKH54_06325"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOC99539.1"
                     /translation="TGVPAGLGDPVFDKLDARLAAALMSIGAVKGVEIGEGFNAARLR
                     GSQMNDQMDAGGCTTNHAGGILGGISNGMPIVCRIAVKPTPGIAEVMDTIDENGEPVE
                     YRRNGRSDPCICIRIVPVAEAMVAIALADSLSSLLSLQDSMQAVRS"
BASE COUNT         1312 a         1861 c         1783 g         1086 t
ORIGIN      
        1 ccgttggaag cctggaaatg atggaacctg aagaattcat tttctcggac atgacctgcg
       61 tgcttcagga cgcaaagaaa tccgtcaacg acgaatactt cttcaaggtg ctgaaggatc
      121 ccctgtcggt gcgctgggga tacaacgaac agtggatgta ccactgcacc ggccagaccg
      181 aatacgacct ggcggtaatc ctgcacttct tcaccgcaca gggaaccgca tgcagccaga
      241 tcgacgatgt gggcatttcc gacgaaaagg ctccgcaggg cgaatacacg acaagttgcg
      301 ccggcaccgg tgaagtcacg gccgagtggg acttccgctg aaagggcgcg ccgcgggggt
      361 aagtgattaa gccgcggtca tcacgacgat ccactgatcg caggtatctg gcccgatcgg
      421 ccgatggaac gtgacgcgga acccacggcc gaatctgttc attctttcaa cgacttcaag
      481 cagttgcgac gaaccccagt tgcgaagatg ctggaagccg ccggcggccg cctggtggct
      541 ggtcaaggcc ttgtgcctgg atctgaccag cggaagcgca agcgattcca tgaacacggc
      601 ctggccgcca ggcttcaggc aacggtgaac cgactcaagc gcggcaccga catcaagaaa
      661 atggttcagg ctgcgcagcg aaaggacatg gtcgaagtaa ccgtcgaatc ccgcgaagtc
      721 ctcgatgccg atctggtgaa cggtcacgcc ggcgatcgtt ttaccggcgt cgggatcagg
      781 atcgaccccg tgatactcga tatgctcgcc atcgggcggg atcggcatga agcccatata
      841 cggaacattg cccagtccga catcaagaac ccgaccgcca agggatttca tcatgtcggc
      901 aagccaacgc tgatcctgat cgaagaagga tgtttcggca ggcagataac aacagcagca
      961 ctggacgaag tcgggacagc gcatgcattc gcgggccagg acaagttgcc gcacatcggt
     1021 cgcgaaatcg tcgatcgacg gcttgccgga cacgtccagg taaagttgac cgcgatcgtg
     1081 caccacatgc ctgatttcgg cctcgtcaaa atcggcggtg tcggttctgc acagcaccgt
     1141 ccgtccgacc tcccgcaggc cgccgtcgcc gtcctcggag gcccggccgg ccgcgttgac
     1201 cagaacgacg cgactcgtcc ccgaagccgc aagcccgtgg tcgcgcgcgc tgcaagcggc
     1261 cggatccagg atgaagccgg gaatcggccg caccggcaca taatcgaatg aattggaaac
     1321 ggccgtgtcg ggattgatgg cccccatgcc tgtcacccct ttatcgggac gatttcgtcc
     1381 ccgccgaacc tggccaggta gtcgcgtcgg gcaccctggc actcggcccg cacataccgg
     1441 cagccttcgc aggccggcag ataggtattg gcgctgatgt cggattccca aaggaagaaa
     1501 cgcgagccgg gtgtaacgat atacacgcga tcctgccgca ggtcatggac gaagtcgtga
     1561 tagccgggga tcatgcaacg cgggatatga cgggaataca cgttcaatcc ggcggccctt
     1621 gaatactcga agacgcgcgc aagttccgga aagatgtcgc ggactcgcgg caggctgtcc
     1681 ggcttgtccg cattcctgtc ggtcagggaa accagccaca acgggaaggt gcgaaccccg
     1741 tggcccgccc agaaggcgac ggtttcagtg agcgttcgcc atgtgtccgc cttgattatc
     1801 atatccagga tcggaacgtg tccggccgcg accaggttgt caacgccgcg cacgaccatg
     1861 tcgaaccatt tcgcgtcgcc catggtggcc gcatacatcc gtggttcatg ccccataacc
     1921 gaaatgtgaa aatcggtcac gcccgcatcg attgcccgac ttgcatagtc ctggtatgcg
     1981 tacatcagcc cgtttgaaga caccttgatc cagtcgaaac cgcgatcccg agcccacctt
     2041 gcaatcggca gcaggtcccg cctgatcgtc ggctcgccgc cgccgaatgc gaccttatgc
     2101 attcccatgg ccctggccgc cgcaagctcg gacaacgctt ccgcggtggt catgtttacc
     2161 gggcgcatcg catcgctgac gctgcagtaa tcgcacttga cgttacattc gtagcccatg
     2221 atcaaatcga ccatgacgtc gccgtaatcg acgccgcggc cacccgcgcc ttcaagcggc
     2281 cccgcgtccc ttcccgtgtc actcatgacg gccaccgtcc tgcatcgcca gttcccccga
     2341 ttccgggaac tcgtaaaacc cgtcgcaaac aaggaaatgc ggacacgacg cgcactgggc
     2401 acgcctgacg cgcctttcgg ccagataccg gaaaagattg acttcctctt cccacacgaa
     2461 aaccatcgtt cgccccagtt tctgcaaatc gttcagaaga tacttctggt acgcgggctc
     2521 gaaaaggcac atctgggaat tgacgacgta gatcttcatg cgatcggcca cggcatcaat
     2581 ggccgcctgg acctgccggg ccgcctggat cggatccggg acaagtcccg ccgacgcggc
     2641 gccgaacggc gtcaggaact ggaagttcat cttgggcacg cccctgtcgg ccatcatccg
     2701 cgcaagaccg accatatgcc ccatattggt cgcgcacacg gtcacgttca cgccgaaatc
     2761 gatttgacgg cccgattgcc cgacaagccg catgacgttg tcgatgccgg cgaccgtttc
     2821 atcgaagctg ccgggcttgc cggtgattgc gtcgtgcacc tgggccgtgt cgccgtgcag
     2881 acttatcagg atgcttgtga cgccgctttt tacaagcgcg gacgcaagtt cccggtcggc
     2941 cagctttctt ccgttcgaag tcacgttaat ctggcggtag ccgaccgcgg cggcatgacc
     3001 gaccacgtcc aggatcccgg gatgaagcat cggctcgccg ccatcgaaat cgacgttgtc
     3061 gatgccgcgg ctgcggtgct cgtccagcat ttcgatgatg cgcgcggtcg ggatggagcg
     3121 gcggacgcgg ttggaaacgg cacagaactg gcatgcattg atacagtcgt aagtcacatt
     3181 gatgatgacc ttggaaggcc gggacgcttc aatccgtcga ttgaaggcca gcatgctgtc
     3241 ttccagctcg cccggaagca tgccgggggg cacgaagccg ggacgatgct ggaacgactc
     3301 gccatccgcg gcaaggaacg gtattccggc ttccctcaac tgcctttcca tgcgtgcgcc
     3361 gggaatcggg gtcgcgaact ggaccgcggg aacggccccg tgcgtttcga aaaggatccg
     3421 ggcgaaatca agcgtacgac gcacgtcggc aagggtttcc cagggaaaac cgatgatgaa
     3481 atgaaccatc agcggcacgc catgccttgc ggcggactcc gcgacccgct cgatgtccgc
     3541 ggggtcaagg cgcttgcaga cacgatcacg cagcgcggcc ggatcggcgg tttcacacga
     3601 aacggaaagc gtcgtgacgc gccccttcat ggccgaaatg gccgcgtcat ccaggcggtc
     3661 tgcccgaagc ccgttcggaa aatcgtagtc caggccaagg tcggcaagga ttttcaacag
     3721 atccgcgaaa tccccccgca gattggcagc atcgtccagg aagagcacat gccggcagga
     3781 gtaagcccgc ttcatcaggt aaagctgatc cgcaagggcg gcaagcggga cggtccggta
     3841 cgccccggag ccatggttgt cgcgccccgg gttcgtcgtg cagaaaacgc agtcatgcgc
     3901 gcagccggcc gaagccagca tgggaagcga acccgcgccg acagcgaacg gattcggacg
     3961 accgctttcc ccgaaaaatc ggacgttgaa ggcatggcgg cttgtaatgt caatcgaatc
     4021 ccacatcggc gccggctggg gcgggatcgc atcccaggaa acggccgggg acgggatatg
     4081 cctggcgccg gaaagaaccg cgtcaagaag cgcgtcagga tcggcgaagt gcgactcgcc
     4141 cgaatacgac aggtaacagg aaataccggg aaacgcggaa aacacgtcat cgaccgggcg
     4201 ctcgacgtac atcatgccgc caacgccaca gtcggcaagg acaatcggaa cgccggggaa
     4261 agatgccgcc acatcccgga tcatcgcggc cgcgggaacc cttgatgccc acaggaaggg
     4321 atggttggcg acgacgacga tgtcgggcga caacccgtcg ataagttcaa cggccccgac
     4381 ggcaacccgg ccaaaaggca cgccggcgac ttgcaggccg ccttccgtgt gccagccgga
     4441 tgtcgggcgc gcaagcgcat caaaaaccgt gacgcgccat cccgccgcgg caaccctggc
     4501 cgccgaacag agcgccccca ggtcggccgc ccatggataa tcgatgaagt cgccttcaag
     4561 aaccatcggc ggaacgatga agagcattga tctggacggc atccggcacc ccgacgcgaa
     4621 aatcgcgacc ttccactgac gcatgattct aacctttcgg acgaattact gacgactggg
     4681 aataacctgc tgaaaaattt tgttacaaat cgaaaaaaag gcttgccgga aaattgtttg
     4741 acgcacacaa tccaaatgaa gacgaaaggc ggctatccga tgcgcgacct ctggcgcagt
     4801 cccctgtccg accacttcat caacgacatc gaacttttcg gacttgcggc cggtttgcga
     4861 ccggtggcga agaaagaagg tcgtgacgcc gatgaaatcc tgaagatttc cgggccgatg
     4921 cgcgatattg gctttctggt tgtcaggaac ccggaaaccg gttgcgtata tgtttccagg
     4981 gaatccgacc gtatcgatcg gctgatgcaa ctggaaaacg gcgagttcgg tggcggaaac
     5041 ccggaagcca ggatgcaggt cattcgcgaa atcggcatct gccttggtta cccggattgc
     5101 tgcgcgaccg cgttcgcccg ccatcgcatg caggacgacg gttacgtgat gggctgtttc
     5161 attgaatgcg agaaggccgt cgcgcacggc aacattgtcc ggaacatccc atggcagctc
     5221 aacttccttc cgccgatggt cagcccggtc ttcttttacc catgccatgc ccgctgcggc
     5281 caggcgcttg ccctggcaga cagatatatc gccgaaatgg atgctgtgcg cccgggcacg
     5341 gcggccgcgg tcaagcgaca gcttcgcggg gtggtgcttg caacgggccg ctgggatttc
     5401 gtgatgtttg atggaacaat tgaaggctct tcgatcaatt acgattcctg gagaccgccg
     5461 gtcagctttc acccggttcc gctgccaacc gccgcctttt ccgtgttcat ggacgcgctt
     5521 cccgagtcgg gtcgagtgat tccggacgat ccgcgtgtca tcgttctgga atacgtctga
     5581 tttgggacaa gttcgtcagg atctgaccgc ctgcatggaa tcctgcagac tcaacaacga
     5641 tgacaacgag tcggcgagtg ctattgcgac cattgcctcg gcaacaggaa cgatccttat
     5701 gcatatgcat gggtcactgc ggccattgcg gcgatactca accggctcgc cattttcatc
     5761 aattgtatcc ataacttcgg caatgccagg ggtcggcttt acagcaatcc tgcatacaat
     5821 cggcatcccg ttcgatatgc cgcccagtat gccgccggca tgattggtcg tgcaaccgcc
     5881 ggcatccatc tgatcgttca tctggctgcc gcgcaaacgt gccgcgttga agccctcgcc
     5941 gatctctact cccttgacgg cgccaatcga cataagcgcg gcagcaagcc gggcatcaag
     6001 cttgtcgaaa acaggatcgc caagtccggc aggaaccccg gt
//
LOCUS       DAOPPA010000163         6025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14381_length_6025_cov_4.335511, whole genome shotgun sequence.
ACCESSION   DAOPPA010000163 DAOPPA010000000
VERSION     DAOPPA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14381_length_6025_cov_4.335511"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKH54_06330"
     CDS             complement(<1..704)
                     /locus_tag="PKH54_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOC99540.1"
                     /translation="MDRIVVKGAREHNLRNVTVGIPKLRMVVFSGVSGSGKTSLAFDT
                     VFAEGQRRYIESLSSYARLFLGQLEKPKYDSIQGLTPTIAIEQKSASSNPRSTVGTIT
                     EIHDYLRVLFARTGTQHCHMCGRPVSRQEPAQILREIMEMTGGSSDGTKVTILAPVAR
                     NRKGEYRDLFKSLEADGFVRVRIDGGLYRLDEVPQLDRNKKHDIDVVVDRIIVRPGGE
                     ARLTDSIETALKTGRGR"
     gene            955..1716
                     /locus_tag="PKH54_06335"
     CDS             955..1716
                     /locus_tag="PKH54_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOC99541.1"
                     /translation="MELRYAGLTDVGRKRDHNEDNFLVVPENNLFVVCDGMGGHACGE
                     VASQIAVEEIAYFFRQSSGDAEATWPYRPDGRISMAENRLVAGIKWANFKVFETASGS
                     GEKKGMGTTCVAFHVDGDKISVAHVGDSRAYRIRDGRIEQLTTDHSLLEQYKQLAKIS
                     DEEIKNFPYKNIIVRALGMKETVVVDTRLEDFRPGDCYLLCCDGLSGELDDEEILDTC
                     NEFRDDLDRCCSELIGRANTKGGRDNITVVLVKGD"
     gene            1744..3981
                     /locus_tag="PKH54_06340"
     CDS             1744..3981
                     /locus_tag="PKH54_06340"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99542.1"
                     /translation="MKRPMWFLLTVLLVSCGGGPDTLDEGGTDVLPDTGRDTVIIIDY
                     GVQEDLNQPRDIPVEEATDMMAVDEGSGEEDTGVVPGGFLYPCTSDRQCYSNFCVQSS
                     EGMVCSKNCAGTGCPDSWDCRQVLVAGDPVYICVPQNVFLCRPCTTNAECTTSIDVSG
                     DKCVAFGSIGSFCGTQCGGKDDVPCPEGYSCEEVSVVGGSTALQCVPQTGECTCSQLA
                     IDLGASTSCFISNANGLCSGARECLPGGLTNCDARNPAVEACNKVDDDCDGVTDNPGS
                     VGCKSYYIDNDQDGWGIGIQHCVCGDEAPLGYASKDGDCNDSSFGMNPAVAEVCNGMD
                     DDCDGVTDEYGSIGCREYSLDNDGDGFGQPGERRCLCNPTPPFTGTVVGDCDDANAAI
                     CPDSNKCKEKCDGIDNDCDDVTDGENALLCTPYYFDADQDTYGLPSKFKCLCAPTGLY
                     TATRAGDCVDTDPEINSTGDEICNGIDDNCNGKTDEGDVASLCPVPPGVELHGTTVCD
                     KVCKVGACDGATVDGLGNYVPAWYDVNENITDGCECQADKVEAVGGQTCNSAVDLGIV
                     PDSGFRVASDSSKGSVVPSSDVDWYKFRASDPTWNGEPGSCDNYNVRVKFIKNPGSQF
                     MFDVYRGSCAAANQICGGTSNHEWAVNFTSGTGGDRKGECPCSTATAPGCDTPASYAE
                     CMRVESNPHKCGSCPGYGAVNTNVCSNNTADVFIKIFRDPLKTPTCEQYEFEISNGLY
                     PFSGT"
     gene            4111..5877
                     /gene="dnaG"
                     /locus_tag="PKH54_06345"
     CDS             4111..5877
                     /gene="dnaG"
                     /locus_tag="PKH54_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOC99543.1"
                     /translation="MEFDQAVREILNRADIVRLVSEYVQLRKNGQHLWGLCPMHAEKT
                     PSFSVNPSRRMYYCFGCHRGGNAITFLSEVAGLTKREAIERLAAETGVQLPQQRPSDP
                     AEEAAARERSEIMKALVVAQEYFVSSLYAPEGERARDYLSDRGVDEQTAAAYGLGFGG
                     VGGGLIRMLEKRGVPMQIAESAGLVLPSTRGTGYYERFRGRVICPVFNNDGAAVAFSA
                     RILPGDDDGPKYLNSPETAVFRKGSMVFGLYQARQAIRQSRHAIVVEGNFDVISLAAS
                     GVLNVVAPLGTALTPQQVRQLARFAGRVTLFFDGDEAGYKASRRSVSILLEEGVEGYI
                     VSTPSGEDPDSMARRGGRQAIEAAVADARPMVTWLVDSLVGVFGRSPHGIRQVIEEVS
                     GVLAAEKDRIRYGLYREEVARILGMDVRELKGILGGRQTDVPGRGPEVPNSVEVSMLK
                     LLALSPALIDTLLDRGGREYFQSEEAIAVLNRMFDLYASGGDLEGELLAAVSEQGSVM
                     SAVASVITMPTGISDVQAAFDETFLQLRIAFLNRRLNELKQAMRGAVDEDAKMRLLVD
                     VTAVERQLKEAGPSLWRFRGTL"
BASE COUNT         1188 a         1686 c         1892 g         1259 t
ORIGIN      
        1 cgtccgcggc cagtcttcag ggcggtctcg atggaatctg tcaagcgggc ctcgccgccc
       61 ggcctgacga tgattcggtc cacaaccacg tcgatgtcgt gctttttgtt tctgtcgagt
      121 tgcggaacct cgtccaggcg atagaggccg ccgtctatgc ggacccggac gaatccgtcg
      181 gcctccagtg atttgaaaag atccctgtac tctcccttcc tgttgcgggc caccggcgcc
      241 aggatggtca ccttcgtccc gtcgctagat ccgccggtca tttccatgat ttcccgaaga
      301 atctgcgctg gttcctgtcg ggagaccggt ctgccgcaca tgtggcagtg ctgcgttccc
      361 gtccttgcga acagcaccct caggtagtca tgtatctcgg tgatggtgcc gacggtcgat
      421 ctcgggtttg acgaagcgct tttctgttcg atggcaattg tcggcgtcag cccctggatg
      481 ctgtcgtact tcggcttttc aagctgcccc aggaagagcc gggcatagga ggagagcgat
      541 tcgatgtatc ttctctggcc ttccgcgaaa acagtgtcga aagccagcga tgtcttgccc
      601 gaaccgctga cgccggaaaa caccaccatc cgcagtttgg gaatcccaac ggtgacgttc
      661 ctgaggttgt gttcccttgc acccttgacg acgattcgat ccatcgagat gctccctccc
      721 cggcagcaga cgggattata gccagcgcgt cgtccgattc cactgccgcg tgaaaaagtg
      781 gcggacttac ggggcagtct gattcgattc gtcccgaccc gtccttttgc gccggcctgg
      841 atcgtaaggc gttgttttgt tgagtaatcg ttgacttagt atgtgattgc ccgcatagtc
      901 caatgagtgg taccatcacc gctctggggt gaacgcggta tctggaggcg cctgatggaa
      961 ttgcgatacg cggggttgac cgacgtcggc cgcaagaggg atcacaacga agacaatttc
     1021 ctggtggttc ccgaaaacaa cctgtttgtt gtgtgcgacg gaatgggtgg ccacgcctgc
     1081 ggcgaggtgg cttcccagat tgcggtcgag gagatcgcct atttcttccg gcagtcgtcc
     1141 ggcgacgcag aggccacctg gccctacagg ccggacggcc gcatctccat ggcggaaaac
     1201 cgtcttgtcg ccgggatcaa gtgggccaac ttcaaggtct tcgaaacagc ttcgggatcc
     1261 ggcgagaaga agggtatggg aaccacctgc gtcgcgttcc acgttgacgg ggacaagatc
     1321 tcggtcgccc atgtcggcga ttcccgtgcc tacaggattc gcgacggcag gattgaacaa
     1381 ctgacaacgg atcattcgct tctggaacag tacaagcaac tcgccaagat ctccgatgaa
     1441 gagatcaaga attttcccta caaaaacatt atagtacgtg cactgggcat gaaggagacg
     1501 gttgtcgtcg atacccgtct cgaggatttc cggcccggtg attgctacct tctgtgttgc
     1561 gacggtcttt cgggcgaact ggacgacgaa gagatcctgg acacgtgcaa cgagtttcgg
     1621 gacgaccttg atcgctgctg ctccgaactt atcggccgtg ccaacacgaa aggcggtcgt
     1681 gacaacatca cggtggtact ggttaagggt gattaatcct tgaagcttgc tggaggtcgt
     1741 ccgatgaagc ggccgatgtg gttcctgttg accgttctcc ttgtttcctg cggcggcggc
     1801 ccggacacgc ttgacgaagg cggaaccgat gttctgcccg acaccggaag ggacaccgtg
     1861 atcatcatcg actacggcgt tcaggaggat ctcaaccagc cgcgcgacat ccccgtcgag
     1921 gaggccaccg acatgatggc ggtggacgaa ggctccgggg aagaagatac cggcgtcgtt
     1981 ccgggcggat tcctgtaccc gtgcaccagt gatcgtcagt gttattcgaa tttctgcgtg
     2041 cagtcttccg agggcatggt ctgttcgaag aactgcgccg gcaccggttg tcccgattcc
     2101 tgggactgcc gccaggttct tgttgccggc gaccccgttt acatctgcgt cccccagaac
     2161 gtattcctgt gccgtccgtg cacgacaaac gccgaatgca cgacttcgat agatgtttca
     2221 ggcgacaagt gcgtggcctt cgggtcgatt ggcagtttct gcggaaccca gtgcggtgga
     2281 aaggatgacg ttccgtgtcc ggaaggatat tcctgcgaag aggtttcggt agttggggga
     2341 tcgacggccc ttcagtgcgt gccgcagacc ggcgagtgca cctgttcaca gcttgcgata
     2401 gatcttggcg cctcgacttc ctgtttcata tcgaacgcga acggcctgtg ctccggcgcc
     2461 cgcgaatgcc tcccgggcgg gctgaccaac tgcgatgccc gcaatccggc cgtcgaggca
     2521 tgcaacaagg ttgacgacga ttgcgacggc gtaaccgaca atcccggctc ggtcggatgc
     2581 aagtcctatt acatcgataa cgaccaggat ggctggggta tcggaatcca gcactgcgtg
     2641 tgcggcgacg aagctccgct cgggtacgcc agcaaggacg gcgactgcaa cgactcctct
     2701 ttcggaatga atccggcggt cgccgaggtc tgcaacggaa tggacgacga ctgcgacggg
     2761 gtcaccgacg aatacggctc gattggctgc agggagtatt ccctcgacaa cgacggcgat
     2821 ggattcggcc agccggggga acgcaggtgt ctgtgcaatc cgacgccccc cttcaccgga
     2881 accgtggttg gcgactgcga cgacgccaat gcggcgatct gcccggattc gaacaagtgc
     2941 aaggaaaagt gcgacggcat cgacaacgac tgcgacgacg tgacggacgg cgaaaacgcg
     3001 ctcctgtgca ccccgtacta tttcgacgcg gatcaggata cttacgggtt gcccagcaag
     3061 ttcaagtgcc tctgcgcgcc gacgggactc tacaccgcga cccgcgccgg cgactgtgtc
     3121 gataccgacc cggaaatcaa ctcgaccggc gatgaaatct gcaacggaat cgatgacaac
     3181 tgcaacggaa agaccgacga gggcgatgtg gcctcgctct gtccggtgcc gcccggcgtc
     3241 gagcttcatg gaaccaccgt gtgcgacaag gtctgcaagg ttggcgcctg cgacggcgcg
     3301 actgtcgatg gactcggaaa ttacgtcccg gcctggtacg acgttaacga gaacatcacg
     3361 gacggttgtg aatgccaggc cgacaaggtc gaggctgtcg gcggccagac ctgcaactcg
     3421 gccgtggatc tcgggatcgt tcccgacagc ggtttccggg tcgcttcaga cagcagcaag
     3481 ggttccgtgg ttccttcatc cgacgttgac tggtacaagt tccgggcttc ggatccgacc
     3541 tggaacggcg agcctggaag ctgcgacaat tacaacgtcc gcgtcaagtt cataaagaat
     3601 cccggaagcc agttcatgtt cgacgtgtat cgcggatctt gcgccgctgc caaccagatc
     3661 tgcggcggaa cttccaacca cgagtgggcc gtcaacttca cgtccggaac cggcggcgac
     3721 aggaagggcg aatgcccgtg ttcgaccgca acggcccccg ggtgtgatac tccggccagc
     3781 tatgccgagt gcatgagggt cgaatcgaac ccgcacaagt gcggaagctg tccgggatac
     3841 ggtgccgtca acaccaacgt gtgcagcaac aacaccgccg acgttttcat caagatattc
     3901 agggatccgc tgaagacacc cacctgcgaa cagtacgaat tcgagatcag caacggcctg
     3961 tatcccttct ccggcacctg agattatcga acaaaattcc cgctttcata gtttgcggat
     4021 atcctgtatg ctaccgcgcc cgtgcttcct gcccgcgccg cggaaggatc gggttggcgc
     4081 ttttcgtgtc gcgttggtga tcccttgtcc atggaattcg accaggccgt ccgggaaatc
     4141 ctgaatcgcg ccgacatcgt caggctggta tcggagtacg tccagcttcg caagaacggc
     4201 cagcacctat ggggactctg tccgatgcat gccgaaaaga cgccgtcgtt ttcggtgaac
     4261 ccgtccaggc gcatgtatta ctgcttcgga tgccaccgcg gtgggaatgc gattacgttc
     4321 ctctcggaag ttgccggcct taccaaacgg gaagcgattg aacggctggc agccgagacg
     4381 ggggttcagc tcccgcagca gaggccttcc gatcctgccg aggaagccgc ggcgcgggag
     4441 cgttccgaga taatgaaggc cctggtcgtt gcgcaggagt acttcgtttc ctccctttat
     4501 gcacccgaag gcgaaagggc aagggactat ctttccgatc gcggggtgga tgagcagacc
     4561 gcggccgcct atggccttgg tttcggtggc gtcggcggcg gactcatacg gatgcttgag
     4621 aaacgtggcg tcccgatgca gatcgcggag tcagccggcc tggtgctgcc ttcgaccagg
     4681 ggaaccggct actacgaaag gttcaggggc cgggtgattt gtccggtatt caacaatgat
     4741 ggcgcggcgg tcgccttttc cgccaggatt ctgccggggg acgacgacgg acccaagtat
     4801 ctgaacagtc cggagacggc cgtgttccgc aagggctcga tggtattcgg gctctatcag
     4861 gccaggcagg cgataaggca gtcgaggcat gcaatcgtcg tcgagggcaa cttcgacgtg
     4921 atatccctgg ccgcgtcggg agtcctgaac gtggtggcgc cgctcggcac ggcactgacg
     4981 ccacagcagg tcaggcaact ggcaaggttc gccggcaggg ttacgctctt tttcgacggc
     5041 gacgaggccg gctacaaggc ttcaagacgt tcggtctcaa ttcttcttga ggagggcgtc
     5101 gagggttaca tcgtttccac gccttccggc gaggatccgg attccatggc gcgaaggggt
     5161 ggtcggcagg caatcgaagc tgccgtcgcc gatgcgcgtc caatggttac ctggcttgtc
     5221 gattcgcttg tgggagtctt cggaaggagc cctcatggaa taaggcaggt tatcgaggaa
     5281 gtatcggggg ttcttgccgc cgaaaaggat cgaatcaggt acggacttta ccgggaggag
     5341 gtcgccagga tccttgggat ggatgtccgt gaactgaagg gaattcttgg aggtcgtcag
     5401 actgatgttc ccggtcgtgg gccggaagtt cccaattccg ttgaagtaag catgctgaag
     5461 ctgctggcct tgagtccggc gttgattgac acgctgctgg atcggggcgg aagggagtac
     5521 ttccagtccg aggaagcgat tgcggtgttg aaccggatgt ttgacctgta tgcttccggc
     5581 ggcgaccttg agggtgaact gcttgcggcg gtgtccgagc aggggtccgt gatgtcggcg
     5641 gttgcttcgg tgatcacgat gccgaccggt atttctgatg tgcaggccgc ctttgacgag
     5701 acatttcttc agttgaggat tgcgtttctg aatcggcgcc ttaatgaatt gaagcaggcc
     5761 atgcgcgggg ccgtcgacga ggatgccaag atgcgtctgc ttgtcgatgt gaccgccgtc
     5821 gaacgtcagt tgaaggaagc cggtccttcc ttatggagat tcaggggcac tttgtgataa
     5881 aaacgtgcgg cagtcgcacg ggttgatcga ggtggggaat ggaaaagaac gagatcaagc
     5941 gccttgttga catggggaag aagcgtggtg gccacctgac gttcgacgag gtcaatgtcg
     6001 ccatgtcccg gggcaatgta ccggt
//
LOCUS       DAOPPA010000164         5995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14473_length_5995_cov_4.164646, whole genome shotgun sequence.
ACCESSION   DAOPPA010000164 DAOPPA010000000
VERSION     DAOPPA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14473_length_5995_cov_4.164646"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..4468)
                     /locus_tag="PKH54_06350"
     CDS             complement(131..4468)
                     /locus_tag="PKH54_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99544.1"
                     /translation="MDLLAIQINAASHVAVPVRKALLEPGPADATFTLLYAAHRLLRL
                     ACAFGGYDADAQGAGRFDRMIPFVADMISRGTLDLKETGVSTALSDLQVAADELVTVD
                     AYAERTSREWQPALELTMSCLGGLRPLALFLRTAAGDRDGMMHLAVGSGLPAVRRVSQ
                     QGSQVTSILLPDDSLKETGPAMRTGDGIVEPLIPWTGWAGSFETIDGRIVDGAGAATI
                     PVAPFVPASALGRLAAFHAGGSGAPAARIGRTAPGQSAAEYLIISTFGGDDWKTPAGI
                     QAIFDFVIDNGDARLQQAVTHAAAVAIGRMVESLSTSDDAGALAEALISLQQFQEGAA
                     RTATLRRASNLYLEKVGDKTAAQLCLLKAIESAPGDTHQLADLLELASEMPAGDQAWG
                     EAAAEMAGDMVKLGKGLSGRPEAAPVLKTAADLYDIAGREEAAAQAHACVLEADPNAV
                     VSIERVIASILEPVELERECRARLKRAWDSTARGKILSALARALAALGRASEAAESIK
                     EALALLPESNDLLQSARSILLDAADWPSLSGVMNRLLAVAPDAQARTDAAIDMAAIAV
                     DRMKSPAIALAWLEKVDDGKNAAIATRLQQVHAELGMWKEAADDLQRVLSDSNSVQDW
                     IALARIFRDRLGNQSEAARSFRNALERLEGQELVAIALEYAELPGLKDVDGFVATTLN
                     RAIDAADDDIVKASLFKLLGRFNSAGDTAGSDSVQHLEEALKLNPVDCECALKLAGIY
                     LARDQAEKAFSMLEAPARAAARAGDTETEIRLREISAGAAVRCFDHKGAARNLERILE
                     LQPANIDAARRLAGVRAALGNVDGAIEVLNSRATPDGKPAVSDAPLLRELARALSSQK
                     RYAEALARLQEAWAIDSTPSEEVFRELVEAASAAGDRDAMIDWLKRLVNLEQPSERRF
                     TDLIRLGDAMKAQGDLDGATEWYMVAAREEYSRKVALHKALESSIEARQFGRARNILM
                     SIVEEEQDDLKKSDYLLAAALIARDDLNDLNQAASILKEALVRNPFNEQAAAASDQLL
                     LKLERLDELAESLSLRARHYRVSGDSAVLVDTLRKLGDVYENGLHNAMKAAEVFRQIL
                     TVTPDDTAAQKHLAYCLTRIPGSEAEALETLRRVVSGDPTSIESYRAIRDLSILTSDQ
                     DLATRASSALIVLGQGDESDFHMAARNRSASLRLKRDQIPPDAFMRFIAGGLDTRTAR
                     IFAILYNPILKIVPFKTPEEAGLGNVNRIMMDEDGLFQNMADAVGRVFDMELPVFWHA
                     RGSRGLAKAPFAGRAVVVGDDLVSTRRGKDLRFALARAVVSFMPGAELCGVVDAASLR
                     LFFLAALKMSFPDYPLPADGAGAADFVPLLGKLLKEPEKDEIRRILVNFRKLQKPVDL
                     PGFMRSIDNISARAGLFMAADLEVAASRTMEADMILSDMEPEDRLVELASWCVSADYS
                     ELKKLMIQS"
     gene            complement(4589..5356)
                     /locus_tag="PKH54_06355"
     CDS             complement(4589..5356)
                     /locus_tag="PKH54_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99545.1"
                     /translation="MKRILPCLLLALILPLSVRGETVPQLFDAGVSEYRQGNFDRSAG
                     IFLDLVEKYDVVSPDVLANLGSAEFAAGRAGDAMLHLMMAARLDPDGRAGQTAAVNLD
                     RIRSLLNERQEGRSGSAYVFGGYTDPWMALFSWLPPQAALIAFLACWASFFVSLAVRR
                     MLRPESARTLAAIALALTVATGFMAYATSRISGYRTGVVLTESTPLMTSIASTEPSAT
                     LPEGLEFRVMERRGAWVRIRLSSGLTGWIPESEAGIP"
     gene            complement(5353..>5995)
                     /locus_tag="PKH54_06360"
     CDS             complement(5353..>5995)
                     /locus_tag="PKH54_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99546.1"
                     /translation="VPFFSSLTGKYERARSDRIVLTGAGFAAGGKVMEARRDNPPLGI
                     IGESDLKVPEPAVDDEINSGLLAAVMAAPVLFFGVAETLARRRAFLVANGRALRGRRA
                     LAAARAGLRRLRESNTGGQEFWTGLEGLIRVFLDARFGLNTQALTAQEIATALAAAGA
                     SPDDISSLAGELESCAFARFAPASALQGDRSAAIDRVERCLSGLDGSQGGEPG"
BASE COUNT         1100 a         1903 c         1848 g         1144 t
ORIGIN      
        1 ggtcgccgaa caccttgtcc ggtacggcgc gatatacctt gagcccggcc cgcgggcgaa
       61 gctctacgat tcgatttttc cggataccgt cgattacgac cttggcggga cagttgacta
      121 ctagccggct tcaggactgg atcatcagct ttttcaattc ggaataatcc gcggatacgc
      181 accaggatgc cagttcgaca aggcggtctt ccggttccat gtcggaaagg atcatgtccg
      241 cctccattgt cctggaagcc gcgacttcaa ggtcggccgc catgaagagg cccgcccgcg
      301 ccgagatgtt gtcaatcgat ctcatgaatc cgggcagatc gaccggcttc tgcagcttcc
      361 ggaagttgac aaggatgcgc cggatctcgt ccttctccgg ttccttcagc agcttgccca
      421 gaaggggcac gaaatctgcc gcgccggccc catcggctgg aagcgggtaa tcagggaagc
      481 tcatcttcag cgcggccagg aagaaaagtc gcagggaagc ggcatcgacc acgccgcaca
      541 attccgcgcc gggcatgaac gacaccacgg cccttgcaag ggcaaacctc aagtcctttc
      601 cacgccttgt agaaacaaga tcatcgccga caacgaccgc cctgccggca aagggagcct
      661 tggcaagacc gcgcgacccc ctcgcatgcc agaataccgg cagttccatg tcgaacacgc
      721 gaccgaccgc atcggccatg ttctggaaaa ggccatcttc atccatcatg attcgattga
      781 cattgcccag cccggcttcc tcgggtgtct tgaatggcac gatcttcagg atggggttgt
      841 acagaatcgc gaatatcctc gccgttctgg tatccaggcc gccggcgatg aagcgcatga
      901 acgcgtccgg cgggatctgg tcgcgcttca gccgcagcga ggcggatctg ttgcgggccg
      961 ccatgtggaa gtcggattcg tcaccctggc ccaggacgat aagcgcgctg gaggcccttg
     1021 tcgccagatc ctgatcgctg gtcagaatgc tcagatcccg tatggcgcgg taggattcta
     1081 tcgacgtcgg atccccggaa accacccttc gaagggtttc aagcgcctcg gcttcggatc
     1141 ccggaatcct ggtaaggcag tatgccagat gcttttgcgc cgccgtatcg tccggcgtga
     1201 cggtcaggat ctgtcggaag acctcggccg ccttcatggc gttgtggagg ccgttttcat
     1261 agacatcgcc aagcttcctc aacgtatcga ccagtaccgc ggaatcgccc gaaaccctgt
     1321 agtgcctggc acgaagggac agtgattccg caagctcgtc cagtctttcc agcttcagca
     1381 gaagctgatc cgatgccgcg gcggcttgtt cgttgaacgg gttcctgacc agggcctcct
     1441 tcaggatgga agcggcctgg ttcaggtcgt tcaggtcgtc gcgggcaatc agcgctgcag
     1501 ccaggaggta gtcggacttc ttcaggtcgt cctgttcttc ttcaacgatg ctcatcagta
     1561 tgttgcgggc ccgtccgaac tggcgggcct cgatggacga ttccagggcc ttgtgcagcg
     1621 ccacctttcg ggagtactcc tcgcgcgccg ccaccatgta ccattcggtc gcgccgtcga
     1681 ggtcgccttg agccttcatg gcgtcgccga ggcgtatcag gtcggtaaac ctgcgttccg
     1741 aaggctgttc caggttgacc aggcgcttga gccagtcgat catggcatcc ctgtcgcctg
     1801 ccgccgacgc cgcttccaca agttcccgga agacctcctc ggatggcgtg gagtcgattg
     1861 cccaggcttc ctgcagcctg gcgagagcct cggcatatct tttctgggat gaaagggccc
     1921 gggcgagttc gcgcagcagc ggcgcgtccg aaaccgccgg ctttccatcc ggggtggccc
     1981 gggaattaag gacttcgatc gcgccatcga cgttgccaag cgctgcgcgc accccggcca
     2041 gccttcttgc ggcgtcgatg tttgcaggct gcagttcgag tatgcgttcc aggttccttg
     2101 cggcgccctt gtgatcgaag cagcggactg ccgcaccggc cgaaatctcc cgcagacgga
     2161 tttcggtttc cgtgtcaccg gccctggccg cggccctggc cggggcttcc aacatcgaga
     2221 acgccttttc cgcctgatcc ctggcaaggt atattccagc cagtttcaac gcacattcgc
     2281 agtcaacagg gttgagcttc agagcctctt cgagatgctg gacggaatca gaaccggcag
     2341 tatcgcccgc agagttgaaa cgcccaagca gtttgaacag gctggccttg acgatgtcgt
     2401 catcggcagc gtcgatggcc cggttaaggg tagtggccac gaaaccgtcc acatccttga
     2461 gtcccggaag ctcggcgtac tcaagagcga tggcgacgag ttcctgtcct tcaagtcgct
     2521 cgagggcatt tcggaacgac cgtgcggctt cggactggtt tcccagccga tccctgaaaa
     2581 tcctggccag cgcgatccag tcctgaaccg agttcgaatc cgacaagacc ctttgaaggt
     2641 cgtcggcggc ttccttccac atgccgagtt cggcgtgcac ctgctgaagc cgcgtggcga
     2701 tggcggcatt ctttccgtca tcgacctttt caagccaggc aagagcaatc gcgggtgatt
     2761 tcatgcgatc caccgcaatc gcggccatgt cgattgcggc gtcggttctg gcctgggcgt
     2821 ccggggcgac cgcgagcagc cggttcatga cgcccgaaag ggaaggccag tcggcggcat
     2881 caagcaggat gctccttgcc gactgaagca ggtcgtttga ttcaggaagc agcgccagag
     2941 cttccttgat ggattcggcg gcctcggatg cgcggccgag ggcggccagg gcacgggcaa
     3001 gtgcggacag gatttttccc cgggcagtcg agtcccatgc ccgcttcagc ctggccctgc
     3061 attcacgctc aagttcaacc ggttccagaa ttgaggcgat caccctttcg atgcttacga
     3121 cagcgttggg atccgcttca aggacgcagg cgtgggcctg cgccgccgct tcctccctgc
     3181 cggcgatgtc gtacagatcc gccgcggttt tcaggactgg agccgcctcg gggcgaccgg
     3241 acaggccctt tccaagtttc accatgtcgc cggccatctc tgccgccgcc tcgccccagg
     3301 cctgatcacc ggccggcatt tcggaagcca gttccagaag gtcggcaagc tggtgcgtgt
     3361 cgccaggcgc cgattcgatt gccttcagca ggcagagttg cgccgcggtc ttgtccccga
     3421 ccttttccag gtacaggttg gaggcacggc gcaaggtcgc cgtcctcgcc gcgccttcct
     3481 ggaactgctg aagggaaatc agggcctcgg ccagcgcgcc ggcgtcatcc gaagtcgaga
     3541 gggattccac catccggccg atcgcgacgg cagcggcatg ggttacggcc tgctggaggc
     3601 gcgcatcacc gttgtcgatc acgaaatcga agatggcctg gatgccggcc ggggtcttcc
     3661 agtcatcccc gccgaacgtc gaaattatca ggtattcggc tgccgactga cccggtgcgg
     3721 ttcgtccgat gcgggccgca ggcgcgcccg aaccgccggc gtgaaatgcg gccagtcgcc
     3781 cgagggcgga cgccggaacg aacggcgcca ccggaatggt tgcagccccc gccccgtcaa
     3841 cgattcttcc atcgattgtc tcgaatgagc cggcccatcc ggtccatggt atcagcggtt
     3901 cgacaatgcc gtccccggtc cgcattgccg gtccggtttc cttcagcgag tcatccggca
     3961 gcaggattga ggtgacctgc gagccctgct gggagactct cctgactgcc ggaaggccgc
     4021 ttccgaccgc aaggtgcatc attccgtcgc gatcgccggc agccgtcctc aggaacagcg
     4081 ccagcgggcg aagcccgcca aggcaggaca tcgtcaattc aagtgccggc tgccattccc
     4141 tggaggttct ttcggcatac gcgtccaccg tcaccagttc gtcggccgcg acctggaggt
     4201 cggaaagggc cgtcgataca ccggtttcct tcagatccag ggttccgcgc gaaatcatgt
     4261 cggcaacgaa tggaatcatt cggtcgaacc tgccagcccc ctgcgcgtcg gcgtcgtagc
     4321 cgccgaatgc gcaagccagg cgcagcagtc tgtgggccgc gtacagaagg gtgaaagtcg
     4381 cgtcggccgg ccccggctcg agcaatgcct tcctgaccgg aacggctacg tgggacgccg
     4441 cgttaatctg gatagcgaga agatccatgt tccaccccgg gtgttttcaa acttgaaggg
     4501 ctttatttta tcgacagaac gttcaggaac caacacgccg accaaaaatt gttgcaacgc
     4561 gggatgttgc aggctgcgtg acggccggtt aagggatgcc ggcttcagat tcggggatcc
     4621 agcccgtcag tccggaagag agccttatcc tcacccacgc cccccgacgt tccattaccc
     4681 ggaattccag gccctccggc agggttgccg acggttccgt ggatgcaatc gacgtcatca
     4741 gtggagtcga ttcggtcagg acgactcccg ttcggtatcc ggatatcctg cttgtggcat
     4801 aggccatgaa tccggtggcg accgtaagag caagtgcgat ggccgcaaga gtccttgcgc
     4861 tttcgggccg aagcatgcgc ctgacggcca gggaaacgaa gaaggaagcc cagcatgcca
     4921 ggaaggcgat cagggcggct tgtggtggaa gccacgagaa aagggccatc cagggatccg
     4981 tgtacccgcc gaaaacgtat gcggaaccag atctgccttc ctgccgctcg ttcagcagcg
     5041 atcggatcct gtccaggttg acggcggcgg tctggccggc ccttccatcg ggatccagtc
     5101 ttgcggccat catgaggtgg agcatggcat cgccggcacg gccggcggcg aattcggccg
     5161 agccgaggtt cgccagcacg tcgggggaaa caacgtcgta tttttccaca agatcaagaa
     5221 agatgccggc agaccggtcg aagtttccct gtctgtattc ggatacgccg gcgtcaaaaa
     5281 gctggggcac cgtttcgccc cggacggaca acgggaggat gagggccaga agcaggcatg
     5341 gaaggatccg tttcatcccg gctccccccc ctgcgaaccg tccagtccgg aaaggcacct
     5401 ttcgacgcga tcgatggccg ccgaccggtc gccctgaagt gcgctggccg gtgcgaagcg
     5461 cgcgaaggcg caggattcga gctcgccggc caggcttgaa atgtcgtcgg gagatgcgcc
     5521 tgcggccgcc agggccgtgg caatctcctg ggccgtcaag gcctgggtgt tgagcccgaa
     5581 gcgcgcgtcc aggaataccc gaataaggcc ttccaggccg gtccagaatt cctggccccc
     5641 ggtgttcgat tcccgcaggc ggcgcagccc ggcgcgggca gccgccagcg cgcgccttcc
     5701 acggagcgcc cggccgttcg cgaccaggaa cgcccttcgc cgggccagtg tttcggcgac
     5761 cccgaagaaa agaaccggcg ccgccatgac cgccgccagc aggccggagt tgatttcgtc
     5821 gtcaactgcc ggttccggaa ccttgagatc gctttctcca atgatcccca gcggcgggtt
     5881 gtcgcgcctg gcctccatga cctttccgcc ggcggcaaat ccggctccgg tcaggacaat
     5941 cctgtcggat cttgcccgct cgtacttgcc ggtcaggctg ctgaagaacg gcacg
//
LOCUS       DAOPPA010000165         5981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14522_length_5981_cov_4.394533, whole genome shotgun sequence.
ACCESSION   DAOPPA010000165 DAOPPA010000000
VERSION     DAOPPA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_5981_cov_4.394533"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3307
                     /locus_tag="PKH54_06365"
     CDS             <1..3307
                     /locus_tag="PKH54_06365"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99547.1"
                     /translation="EGDAADVAVDTTDVEGDAQDVAVDTTDVEGDAQDVQGDTDVSGG
                     DQGHIDTATDGGTGICGQAVELTDGQELDAVGLPEGTPSAWDTYSCEPDSTWTGPERL
                     YAFTSDCDGFVRFTVTPQGAPDGIDYLPVAVGILEDCSAGSCARLLPPAHPLTVPVDV
                     EPGQVVYLVVEAMDTALPAFKIKAELFCRDCRDDDEDGFDGFNSIFCPQGTDCNDDDA
                     AINPEAGETPCDGVDQDCSGFDACDGIVCADNDGDGYFNYDPVTCPMGQDCNDSNGAI
                     GKSASDIECNGIDEDCDGMDWCPGTGQQCDPCLFMTGCREDHTCIPVIGDGTGFAAYC
                     AKFCERSAECPYGSRCVPEVFEGNGVCLPYMQNTCSGGLMTVKDTCGNQISRKQCAGS
                     CSVEWGICIESCPAPAPLTVGTTVGGRLDGDVSNIGTWPGVPDAPGPEASFTFTAECD
                     GVARLVATEMADMQMFVAVTVTECVNTRTIAIENGDSFIHELEFPVVAGFRMFATVDS
                     TMQGDSGPFTILVEIDCDETGREHCEPCEFDLQCDSGFCHRPGVPSLSTGACLKDCSL
                     NGQCPEESTCTAAGDKFVCMPQTAGDCQGQDLFIQDSCGLSYLARTCPDGWQCNAGQA
                     DCTNSCVDGDSDGFFGHDDDACPTGNDCDDENSAINPAANDIRCNAIDEDCSGADNCS
                     DAGQCEQCEGGHCGPGFYCFKDPNYDVPGICLADCTGTMECPMAPNWNLSCWSWMSGI
                     GDDSYYCRPKITIECDGKTSLIKDGCDRTVETAACDIDCFDEFGCSNYCTATQPHPGC
                     GKTVHGTTVGAPDRTNYYYNATDTRDDLTGPEVQYTFTASCTGEFNAVLTNATNWLAL
                     VVVKNASGKCRSSTMIDYDVFLDTPDVVAFTATQGDEFTLIVDGMDGAEGEFDLTVNC
                     LCGANCADNDSDGFIDRHATDCPSGSDCDGGNAAIHPGAAEIKCNDIDEDCTGYDDCR
                     SDACTADVELGNLAAPVSRTDSRMGDTDQMTFYDGPCTSSYPRFNIETVYHFNPACSG
                     TLTASVSEPQDNQYDIYLLADTCRADLCTKVAWYDLTAEVAAGTDYYLVIDGNSSATT
                     NSWKLELALQCAD"
     gene            complement(3326..4078)
                     /locus_tag="PKH54_06370"
     CDS             complement(3326..4078)
                     /locus_tag="PKH54_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99548.1"
                     /translation="MTTASKLLAATACAMLFVAPASVLALDSTETFPEGEMDLEFFLG
                     LHGLKGGRHFDGLEFETSLNYGVIDRFSIYGRGQVGSDGDFDAVSGGAAFGIIGNPLD
                     TDHFDFDLMLEFGFGDFGLALIPSVELNFDLKPDLRLWGVYIRIEQILGADMDAGGSG
                     FQAFSAVTAGTYWTIKDGHKLLAEYDMQFANNPAAGERVVDVGRVALGYNVGIGSNVD
                     MINEIHCDVPQSGEKPAFGFSVGFKVHAINKR"
     gene            complement(4244..5689)
                     /locus_tag="PKH54_06375"
     CDS             complement(4244..5689)
                     /locus_tag="PKH54_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOC99549.1"
                     /translation="MVLAFIIVYMVVTVVVGLLASRLVKNSTDFIVAGRRLPLYMTAT
                     ALFATWFGAETMLGASSRINNEGMLGTIEDPFGAALCLLLLGLFFARPLYRSGYLTFG
                     DFFKGRYDRKSEILSSLFIVLSYFGWVAAQIVALGLVVNIVTGFPVHWSMILASSVLI
                     LYTFFGGMWAVSINDFIQTIMIVGALVVVTIVLGKQAGGVEAVLSDRPDGFYNLWPEP
                     KVVHVLEYLTAWMAIGLGSIPQQDLFQRVCSARSEKTAVRACYIGSFMYLTIAAMPLF
                     IAMTVRNLHPELLNMADHQAILPTLIKLYTNPWIQAFFWGALLSAILSTASAAILAPS
                     VILSENLIRPLLPKLNDRQFLWLTRACVLIISTVAILFALPQDPTAAHAADSGMPMIW
                     ELVAESYTATLVSLLVPLTAGIYWKRANSRGAFAAMVGGILVWALFNWVLHAEFMAKI
                     AGFVASIVFMVAGSLFRPVRTGRDSFPHLQQ"
BASE COUNT         1365 a         1778 c         1783 g         1055 t
ORIGIN      
        1 cgaaggcgac gccgcggatg tcgcggttga cacgacggat gtcgaaggcg acgcccagga
       61 tgtcgcggtt gatacgacgg atgtcgaagg cgacgcccag gatgtgcagg gcgacaccga
      121 tgtgtccggt ggcgaccaag gccatattga caccgcgacc gatggcggca ccggcatctg
      181 cggacaggcc gtcgaactga cagacggcca ggaacttgac gccgtgggac ttccggaagg
      241 taccccgtcg gcctgggaca cctattcctg cgaaccggat tcgacctgga ccggccccga
      301 acgactttac gcattcacct cggattgcga cggcttcgtg cgcttcacgg tgaccccgca
      361 gggtgcgccc gacggcatcg actaccttcc cgtggccgtc ggaatccttg aagactgttc
      421 ggcaggcagt tgcgcaaggt tgctgccccc ggctcatccc ctgaccgtgc ctgtcgatgt
      481 tgaaccggga caggtggtgt acctggtcgt cgaggcaatg gataccgcgc tgccggcatt
      541 caaaatcaag gccgaactgt tctgccgcga ctgccgggac gacgacgagg acgggtttga
      601 cggattcaat tcgatcttct gcccgcaggg caccgactgc aacgacgacg acgccgcgat
      661 caaccccgaa gcaggcgaaa ccccatgcga cggcgttgac caggactgca gcgggttcga
      721 cgcatgtgat ggaatcgtgt gcgccgacaa cgacggcgac ggatatttca attacgaccc
      781 ggtcacctgc cccatgggac aggactgtaa cgacagcaac ggcgccatcg gcaagtcggc
      841 cagtgacatc gaatgcaacg gcatcgacga agactgcgac ggaatggact ggtgccccgg
      901 caccggccag cagtgcgatc cctgcctttt catgaccggc tgccgcgaag accacacatg
      961 tattccggtc atcggggacg gtaccggttt tgcggcctac tgcgcgaagt tctgcgaaag
     1021 atccgccgag tgtccgtacg gttcccgttg cgtgccggaa gtgttcgaag gcaacggcgt
     1081 atgcctgccc tatatgcaaa acacgtgctc cggcggcttg atgacggtca aagacacatg
     1141 cggcaaccag atatcaagga aacaatgcgc gggatcgtgt tccgtcgagt ggggcatctg
     1201 catcgaatcg tgcccggcgc cagctccgtt aaccgtcggg acgactgttg ggggcaggct
     1261 tgacggcgac gtttcaaaca tcggcacgtg gcccggcgtc ccggatgccc ccgggcccga
     1321 ggcgtcgttc acgttcacgg cggaatgcga cggcgtcgcc aggcttgtcg cgaccgaaat
     1381 ggccgacatg cagatgttcg tcgccgttac cgtcacggaa tgtgtaaaca ccaggacgat
     1441 cgccatcgaa aacggcgatt ccttcataca cgaactggaa ttccccgtgg tggccggctt
     1501 ccggatgttc gcgaccgtcg attcgacgat gcagggcgac agcggcccgt tcaccattct
     1561 tgttgaaatc gactgcgacg aaaccggacg ggaacactgc gagccttgcg aattcgacct
     1621 gcagtgcgac tccggattct gccaccggcc cggcgttcct tcactttcaa ccggggcatg
     1681 tcttaaggat tgctccctga atggccagtg tcccgaagaa tctacctgca ccgcggcggg
     1741 cgacaaattc gtgtgcatgc cgcagacggc tggtgactgc cagggccagg atctttttat
     1801 tcaagattcc tgcggacttt catatctggc aaggacttgc cccgacggat ggcagtgcaa
     1861 cgccggccag gctgactgca caaactcttg cgtcgatggc gacagcgacg gcttcttcgg
     1921 acacgacgac gatgcatgtc cgaccggcaa cgactgcgac gacgagaatt cagccatcaa
     1981 tcccgccgca aacgacatca ggtgcaacgc aatcgacgaa gactgcagcg gggccgacaa
     2041 ctgcagcgac gccggccaat gtgaacaatg cgagggcggc cactgcggcc ccggattcta
     2101 ctgcttcaag gatccgaact acgacgtccc cggaatctgc ctggccgact gcaccggcac
     2161 gatggaatgc ccgatggcgc cgaactggaa cctgtcgtgc tggtcctgga tgtccgggat
     2221 cggcgacgac agctattact gccggccgaa aatcacaatc gagtgcgacg gcaagacgtc
     2281 actgatcaag gacggctgcg accgtaccgt cgaaacggcc gcctgcgata tcgactgctt
     2341 tgacgaattc gggtgttcga actactgcac cgccacgcag ccccatccag gctgcggcaa
     2401 gaccgtccac ggaaccaccg tcggcgcgcc ggaccgcacc aactactact acaacgccac
     2461 ggacactcgc gacgatctga cgggcccgga agttcagtac acgttcacgg cgtcctgcac
     2521 cggcgaattc aacgcggtgc tgacgaacgc cacgaactgg ctggcgctgg tggtcgtgaa
     2581 aaatgcgtcc ggcaaatgcc gcagcagcac catgatcgat tacgacgtgt tcctggacac
     2641 cccggacgtg gttgctttca cggcgaccca gggtgacgaa ttcacgctga tcgtggatgg
     2701 aatggacggc gccgagggcg aattcgacct gacggtgaac tgcctttgcg gggcgaactg
     2761 cgcggacaat gacagtgacg gattcatcga cagacacgcg accgactgcc cttccggaag
     2821 cgactgcgat ggtggcaatg ccgcgatcca ccccggggcg gccgaaatca agtgcaacga
     2881 catcgacgag gactgcaccg gttacgacga ctgccgctca gacgcgtgca cggcggatgt
     2941 cgaactgggc aaccttgccg cgccggtttc aagaacggac tcgcggatgg gtgacaccga
     3001 tcagatgacg ttctatgacg gcccctgcac atcgtcatac cccaggttca acatcgaaac
     3061 tgtctatcat ttcaatcccg catgctccgg cacccttacc gcttcggttt cagagccgca
     3121 ggacaaccag tacgacatct acctgctggc cgacacgtgt cgtgccgacc tgtgcacgaa
     3181 agtcgcctgg tacgacctga ccgcggaagt ggcggccgga accgactatt acctggttat
     3241 cgacgggaac tcgtccgcaa cgaccaattc gtggaaactg gaattggcgc ttcagtgcgc
     3301 ggattgatgc gaagccagga gactgtcaac gcttgttgat ggcgtgaacc ttgaaaccga
     3361 ccgagaaacc aaacgccggc ttttcaccgg attgcggcac gtcacagtga atttcattga
     3421 tcatatcgac gtttgatccg attccaacgt tgtatccaag ggcaaccctg ccgacatcga
     3481 ccacccgttc gccggcggca ggattgttgg caaactgcat gtcgtattcc gcaagcagtt
     3541 tgtggccatc cttgatcgtc cagtaggtac cggcggtgac cgccgaaaat gcctggaaac
     3601 ccgagccgcc ggcgtccatg tccgcgccaa gaatttgctc gatgcggatg taaacacccc
     3661 aaaggcgcag atccggcttc aaatcgaaat tcagttcaac cgatgggatc agtgccagcc
     3721 cgaaatcacc gaacccgaat tccagcatca gatcaaagtc aaaatggtcg gtatcgagcg
     3781 gattcccgat tatgccgaag gccgcgccac cggaaaccgc atcgaagtcg ccatcgctgc
     3841 cgacctgacc gcgcccgtat attgagaagc gatcgataac gccataatta agggatgtct
     3901 cgaattcgag tccgtcgaaa tgacggccgc ccttcaggcc gtgcagcccc aggaaaaatt
     3961 ccagatccat ttccccttca ggaaacgtct ctgtcgaatc aagcgccagg accgaggccg
     4021 gtgccacgaa cagcatggcg caggcggtcg cggcaagcag cttggatgcc gtcgtcattg
     4081 atgaacccct ttcaatgcgt attcggtgac agccctgtcc catcgggacg acgaacggta
     4141 acacaaagta ataccgcatg ccacaacaaa caaaaaacct cttttatttc aattacttga
     4201 aagattcgaa ctcttccgcc aaggctgatt caggacgact gattcactgt tgaagatgcg
     4261 ggaaagaatc gcgtcccgtg cgcacgggcc tgaacagcga cccggcaacc atgaagacga
     4321 tgctggccac gaatcccgcg atctttgcca tgaattcggc gtgcaggacc cagttgaaca
     4381 gcgcccagac aagaatcccg ccaaccatgg ccgcgaaggc cccgcgtgaa ttggcccgct
     4441 tccagtagat gccggcggtc agggggacaa gcagcgatac cagcgtcgcg gtgtaggatt
     4501 cggcaaccag ttcccagatc atcggcatcc cggaatcggc ggcgtgggcg gccgtcggat
     4561 cctgcggcag ggcaaaaagg atcgctacag tcgagatgat cagtacgcac gcccgcgtaa
     4621 gccacaggaa ctggcgatcg ttcaacttcg gcagcagggg ccggataagg ttttcggaaa
     4681 ggatgactga aggcgccagg atggccgccg aagcagtcga gaggatggcc gaaagaagcg
     4741 ctccccagaa aaaggcctga atccagggat tggtgtagag cttaatcaga gtcggcagga
     4801 tcgcctggtg atccgccatg ttcagaagct cggggtgaag gttccgcacc gtcatcgcaa
     4861 tgaaaagcgg catcgcggcg atcgtgaggt acatgaatga tccgatgtag caggcgcgca
     4921 cggcggtctt ttcactgcgg gccgaacaca cccgctggaa caggtcctgc tgggggatgg
     4981 atccaaggcc gattgccatc caggccgtca gatattcaag gacgtggacg accttcggtt
     5041 cgggccagag gttgtaaaac ccgtcgggcc ggtcggacag cacggcttcg acaccaccgg
     5101 cctgttttcc aagaactatg gtcaccacga ccagcgcgcc gacgatcatg attgtctgga
     5161 tgaagtcgtt tatggaaacc gcccacatgc cgccgaagaa cgtgtacaga atcagaaccg
     5221 aggaggcgag aatcatggac cagtgaaccg gaaacccggt gacgatgttg acgaccagcc
     5281 ccagcgcgac tatctgcgcg gccacccacc cgaaatagga caggacgatg aatagtgacg
     5341 acagaatttc cgacttccgg tcataacgcc ccttgaagaa atccccgaaa gtcaggtaac
     5401 cggatcggta aagcggacgc gcgaagaaaa gtccgagcag gagcaggcag agtgcggcac
     5461 cgaacggatc ctcgattgtg cccagcatgc cttcgttgtt gatcctggaa ctggcgccaa
     5521 gcatggtttc ggcgccgaac cacgtcgcga aaagggccgt cgcggtcatg tacaggggca
     5581 gacggcgacc ggcgacgatg aagtccgtgg aattcttgac aaggcggctg gccagcaggc
     5641 cgacgaccac cgtgaccacc atatatacga tgatgaaagc cagcaccata ccggcgcacc
     5701 tcgcaaagac gggattccct tgatctggcc acgaaatcaa gcggattttt gcagtcacgg
     5761 gcggtgtgcg tcaactgaaa aattgcccac tggataaggg cgctcgaggc aggggcggat
     5821 gtattcagag tgtgtaaggg cgaatgaaat ttcgcccgcg ggaacagggc ccgggcaacg
     5881 tgcttatccg tagcgggcgg atgtattccg aaatgtacgg gcgaatgaaa attcgcccgc
     5941 gggaacgcgg ctacgctatg tacttgccgc gcttcgtgta g
//
LOCUS       DAOPPA010000166         5970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14557_length_5970_cov_4.430769, whole genome shotgun sequence.
ACCESSION   DAOPPA010000166 DAOPPA010000000
VERSION     DAOPPA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14557_length_5970_cov_4.430769"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKH54_06380"
     CDS             <1..631
                     /locus_tag="PKH54_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99550.1"
                     /translation="AVSVMSATSMASAQSGGRGGWMTQGAEILPMGKHAFEARVGWPS
                     TNLGVHIPVMSKLEVTPFLTMDYLGYAVRNWVGTFGDTFGVQLKGNLWQQGQHAISLG
                     ADLGIAMNYIGVFDAAFQIGGPELKYSYRWNNPRIAVIAGFRMPIRVWFRAECADIPM
                     LFNAGFEYNVIPNLNIHANLEFGPYVLATAAGAIADGSVGFQFGISYLW"
     gene            complement(707..1690)
                     /locus_tag="PKH54_06385"
     CDS             complement(707..1690)
                     /locus_tag="PKH54_06385"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOC99551.1"
                     /translation="MANLKTRYMGLEIDSPLVAGSSGLTRDAAGVARLEGAGIGAVVL
                     KSIFEEQVTHEVDGMAGPGTGIDAHPEAAEYIRAYGTAHAEENYLERITECAAAVRIP
                     VIASIHCVQAGKWVDFARKAQQAGASGIELNVFVPPFDASVDGRQIEQLYIDIADSVR
                     RVVTVPVSMKIGYHFTGMANFLKALSGHVDALVLFNRFFRMTIDTETMKLVPSVPYSE
                     KHEHEISLRWVSGLFGVLGCDIAASTGINSADVAVKMILAGASAVQVSSVLYQDGVDS
                     VGTINKGISDWMDRHGFSSIEDFRGSVSRNYSTNQSQFDRVQFMKFSVGHE"
     gene            1843..2574
                     /locus_tag="PKH54_06390"
     CDS             1843..2574
                     /locus_tag="PKH54_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC99552.1"
                     /translation="MKTAIISDVHANLEALTSVMAEIDRLGVGRIICLGDVVGYGGSP
                     NECADIVRERCEVTLMGNHDAAVTGAMDVAYYYQGARDAIEWTRGQLADRNYDWLYSL
                     PYSWADRDTAFYHAAPVMPSGFFYVVQEAEAQPHVELAPKLKKVNFIGHAHLTLIFGL
                     IDGQPVEVDASRVTAASEARFIVNVGSVGQPRDRDPRACFATWDDEASRLEYHRVQYD
                     IKKAAGRIVDAGLEQKFAERLFIGV"
     gene            complement(2576..3748)
                     /locus_tag="PKH54_06395"
     CDS             complement(2576..3748)
                     /locus_tag="PKH54_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOC99553.1"
                     /translation="MKAYLDNMASTPADPDVMNRAAARLAGIFGNPHSTLHPFGREAA
                     AVLSEARALVAGAISARPDEIVFTPSATIADNLAVLGIARARKRRGRHIVVSAIEHPA
                     VLMAALHLRQEEDFEVDLVMPDQGGIVRPEAVEALLRPDTTLVSVMAVNNEIGTIQPI
                     QAIASVAKGCGAFFHTDASQAPGRISLDFVRHADLVTLSSHKVHGPRGAGALMIRRRR
                     DINIKPLFFGGGQEGGLCSGTANVDAALAFGMALELAVTRREVDSAHVARLSAELARG
                     VMDAFPGSVVVGDREMSIPHCLSIRLEGIPGETFVSRMARVGVAVSLGSACHGDPAAP
                     SHVLTAIGMTAAEARRTVRFGLGRFTTAEEIAYAEQMATSLAGELLSGRQAAEARP"
     gene            3908..5374
                     /locus_tag="PKH54_06400"
     CDS             3908..5374
                     /locus_tag="PKH54_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99554.1"
                     /translation="MNEYFAVGVVPVSSDTAPKAMTTVKLQVFLPLERELFFTGVVIM
                     LRPGGFLVQLDTPPDMGFLNGTLRDAAISHTREAAPAPKPVGDNIFDGAAADPLRTTA
                     RTERMGALTDPGHRQADDSDSRQTGDRGGTIFGHSSISAAAKAVHAAQPAGGGDETEA
                     PATARREKTRPGVRKPPRPVPGDEPSIPDDEFDISISAVDTVTGQPISSRAKTGRHKR
                     PSFETYQFGRDPAPIDEDIPVLTEAPPVTQTRRQEYHDVNRTSKMKALKPEIPQPAST
                     VDELFEMAATAGGTGLIDAISGPSPELATDDDTADGRTRAIRTPIDRDSMPDQEDSAP
                     SEQDPRDPDPFDDDKSGQLDRELAVSIREMTVPEKQRLARQGRRIARRILIRDNDKTV
                     HKFVFFNPEVKVDEVVEYTRSNSLASDAIEFICTNKQWMESHDVILNIVRNNSTPLEL
                     AVRFVTKLTQNDWRMLARPGAARPAIMNQARRLLQGCE"
     gene            5440..>5970
                     /locus_tag="PKH54_06405"
     CDS             5440..>5970
                     /locus_tag="PKH54_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOC99555.1"
                     /translation="MMKFPSALRTTVLKLFVLLVAAGIAATMHLVVLPAPQPAPTAPQ
                     PDGISSPQLLEAARKFTGFMVARPYSFNDRISGTRLADDSDATVGYVIDTTSLPGLAT
                     GYNGKVPVAIVLDADGLVAGIAPLANLETPSYMDIVESSGFLDVLKGRTIAQALTSTD
                     VDSVSGATFTSQAMISA"
BASE COUNT         1258 a         1895 c         1699 g         1118 t
ORIGIN      
        1 ggcggtttcg gttatgtcag ccacgtcgat ggcatccgcc cagtccggcg gccgtggcgg
       61 ctggatgacc cagggcgccg aaatcctgcc gatgggcaaa catgcattcg aggcccgcgt
      121 cggctggccg tcaaccaatc tgggcgttca catcccagtt atgtccaagc tcgaggtcac
      181 gccgttcctg accatggatt accttggcta tgccgttcgt aactgggttg ggacgtttgg
      241 tgacaccttc ggcgttcagt tgaagggaaa cctgtggcag cagggccagc acgcaatctc
      301 cctgggtgcc gacctgggca ttgcgatgaa ctacattggt gtgttcgacg cggccttcca
      361 gattggcgga ccggaactga agtacagcta ccgctggaac aacccgcgca tcgccgttat
      421 cgccggcttc cggatgccga tccgcgtctg gttccgtgcg gaatgcgccg acatcccgat
      481 gctgttcaac gccggatttg aatacaacgt cattccgaac ctgaacatcc acgcgaatct
      541 ggaattcggg ccgtacgtcc tggcgaccgc ggccggggcc attgccgacg gttcggtcgg
      601 cttccagttc ggtatttcct acctctggta ggatttatcc gccactgaca cagaagtcac
      661 accgccacca gcacataaaa tcaaccgaca atatcgaaga aacgctctac tcgtggccga
      721 cgctgaactt catgaactgc acgcggtcga actgtgactg atttgtggag tagttgcggc
      781 tgaccgagcc acggaaatcc tcgattgacg agaatccgtg acgatccatc caatccgaaa
      841 tccccttgtt gatggttccg acagaatcga caccgtcctg gtaaagtacc gaagaaacct
      901 ggacggccga agcgccggca agaatcatct tcacggccac gtccgcggaa tttatgcctg
      961 tcgatgcggc gatgtcgcaa ccaagtaccc cgaaaagccc tgatacccac cgcagcgaaa
     1021 tctcgtgttc gtgtttttcg ctgtacggaa ccgaaggcac cagcttcatg gtctcggtgt
     1081 cgatcgtcat gcggaaaaag cggttgaaaa gcaccagggc atcgacgtgc ccggacagcg
     1141 ccttcaggaa attcgccatc cccgtgaaat gataaccaat cttcatcgac accggcaccg
     1201 tgaccacgcg ccttacgctg tccgcgatgt cgatgtaaag ctgctcgatc tggcgcccat
     1261 cgacagaggc gtcaaagggc ggcacaaata cgttcagttc gattcccgaa gcgccggcct
     1321 gctgcgcctt ccgcgcaaaa tcgacccact tacccgcctg gacgcaatga atgcttgcaa
     1381 ttacaggaat cctgacggca gcggcacatt cggtgatcct ttccaggtag ttttcctcgg
     1441 cgtgggccgt gccgtatgcc cgaatatatt cagccgcctc cggatgcgca tcgatccctg
     1501 ttccgggacc agccatgcca tcgacttcgt gagtgacctg ctcctcgaaa atcgacttca
     1561 gcacaaccgc gccgatgcct gcgccttcaa gcctggccac gccggccgcg tcgcgtgtca
     1621 gcccgctgct gcccgcgaca agcggactgt caatttcaag ccccatgtac ctggtcttca
     1681 gatttgccac gattcctccg gagttcgtca agataccggt cgaatcttat ccaaaaaacc
     1741 aaaaactgga atatgaaact gaaacatgac agatttgcct gaatgcgaat acaattcgct
     1801 tcgtccggtg aatgcaacgt ctggtttcaa gcagggtgcg cgatgaagac agccatcatt
     1861 tccgacgttc acgcaaacct cgaggcactg acttcggtca tggccgaaat tgaccggctg
     1921 ggtgtcgggc gaatcatctg ccttggtgac gtcgtcggat acggcggaag tccgaacgaa
     1981 tgtgccgaca tcgtccgcga acgctgtgaa gtgacgctga tgggcaatca cgacgcagcg
     2041 gtgaccgggg ccatggatgt cgcctattat tatcagggtg cccgcgacgc gatcgaatgg
     2101 acaaggggcc agcttgctga tcggaactat gactggctct attccctgcc ctattcctgg
     2161 gccgaccggg acacggcctt ctaccatgcg gcaccggtga tgccgtccgg cttcttctac
     2221 gttgtccagg aggccgaagc ccagccccat gtggaacttg cgccaaagct gaaaaaggtg
     2281 aacttcatcg ggcacgccca cctgacgctc attttcggcc tgattgacgg acagccggtc
     2341 gaggtcgatg catccagggt aaccgccgcg tcggaggcca gattcatcgt caatgtggga
     2401 agcgtcggcc agccgcgcga ccgcgatccc agagcctgct tcgcgacatg ggatgacgag
     2461 gcatcaagac tcgagtatca ccgcgtgcaa tacgacatca agaaggctgc cggcaggatc
     2521 gtggatgccg gtcttgaaca gaagttcgcg gaacgtcttt tcatcggcgt ctgattcatg
     2581 gtcgggcttc ggcggcctgc cgacccgaaa gcaactcacc cgcaagcgac gttgccattt
     2641 gttccgcgta agcaatttct tcggccgtgg tgaatctgcc aagaccgaaa cgcactgttc
     2701 gccgcgcctc ggccgccgtc atcccgattg ccgtcagcac atgcgacggc gccgcgggat
     2761 ccccatggca cgcgctgccc agcgaaaccg caacgccgac ccgcgccatc cttgaaacga
     2821 atgtctcgcc ggggatgcct tccagcctga ttgaaagaca atgcggaatc gacatttcgc
     2881 ggtcaccgac gacaaccgaa ccgggaaagg catccatcac gccgcgggcc agttcggccg
     2941 acaggcgggc aacatgcgcc gaatcgactt cgcgccgcgt gacggcaagc tccagggcca
     3001 tcccgaaagc cagcgccgca tcgacattgg ccgttccgga acagagcccg ccttcctggc
     3061 cgccaccgaa gaacaagggc ttaatattga tatcgcgccg acgccgtatc atcagcgcgc
     3121 cggccccgcg cgggccatga accttatggg acgaaagcgt gaccagatcg gcatgccgga
     3181 cgaaatcaag actgatccgc ccgggggcct gcgaagcgtc tgtgtggaaa aaggcgccgc
     3241 accccttcgc caccgacgca atggcctgaa tcggctgaat cgtcccgatc tcgttgttta
     3301 ccgccatgac cgaaaccagc gtcgtgtcgg gccgcagaag cgcctcgacc gcttcgggcc
     3361 ttacgattcc gccctgatcg ggcatgacca gatctacctc gaaatcctct tcctgccgaa
     3421 gatgaagcgc cgccatcagg acggccggat gttcgatggc gctcacgacg atgtgacggc
     3481 cgcgacgctt ccttgcccga gcgattccaa ggacggccag gttgtcggca atcgtggccg
     3541 aaggcgtgaa cacgatttcg tccggcctgg ccgatatcgc acccgcgacc agagcgcgtg
     3601 cctcggacag caccgccgcg gcctcgcggc caaaaggatg aagggtcgaa tgcgggtttc
     3661 caaaaatacc ggccagcctg gctgccgcgc gattcatcac atcgggatcg gccggagttg
     3721 acgccatgtt gtccaggtac gccttcatgc cgcggattct gccagaaacc ggccccatag
     3781 tgaagcttgc gaaatcctga caggtgctgc tattgaccgg cgccgcgtat tttgcgatcc
     3841 ttcgcccatg gctgccccga accaatacat cgtcgatttc acttcgccgg aagctctgaa
     3901 ggcggtgttg aacgaatact ttgccgtcgg cgtggttccc gtcagttccg acacggcccc
     3961 gaaggccatg accaccgtca agctgcaagt attcctgccg ctcgagcgcg aactgttctt
     4021 caccggggtc gtcatcatgc tgcggcccgg cggcttcctt gtgcaactgg acaccccgcc
     4081 ggacatgggc ttccttaacg gaaccttgag ggacgccgca atctcgcaca cgcgtgaagc
     4141 ggccccggcg ccgaagccgg tcggtgacaa catctttgac ggggcggcgg cggatcctct
     4201 tcgaacaacg gcccgcacgg aaaggatggg tgcccttacg gatccgggac accgacaggc
     4261 cgacgactcg gattcacgcc agacaggcga tcgtggtgga acgattttcg gccattccag
     4321 catatctgcg gccgcgaagg cggttcatgc cgcacagccg gccggcggcg gcgacgagac
     4381 agaggccccg gcgacggcgc gacgcgaaaa gacccgcccc ggtgtgcgga aaccgccgcg
     4441 accggttccg ggtgacgagc cgtcgattcc cgatgacgaa ttcgacattt cgatttccgc
     4501 cgtcgatacg gtgacaggcc agccgatttc ttccagggcg aagacgggcc gccacaagcg
     4561 cccatcgttc gaaacctatc agttcggacg ggatccggcc cccattgacg aagatatccc
     4621 cgtgcttacc gaggctccgc cggttaccca gaccaggcgc caggaatacc acgatgtcaa
     4681 caggacatcg aagatgaagg cgctgaagcc cgaaattcca cagccggcaa gcaccgtgga
     4741 tgaactgttc gagatggcgg cgaccgcggg cggaaccggg ttgatcgacg cgatctcggg
     4801 gccatccccg gaattggcca cggacgatga caccgcggac ggacgaacca gggccattcg
     4861 caccccgatt gaccgggatt cgatgcccga ccaggaagat tcagcgccgt cggaacagga
     4921 tccacgggat ccggatccgt tcgacgacga caagtccggg cagctcgatc gtgaactggc
     4981 cgtcagcatc cgggaaatga ccgttccgga aaagcagcgc cttgcccgcc agggaagacg
     5041 catcgctcgg cgcatactca ttcgcgacaa cgacaaaacc gttcacaaat tcgtcttttt
     5101 caacccggaa gtgaaagttg acgaggtcgt cgaatacacg agatcgaact cgctggcatc
     5161 cgacgccatc gaattcatct gcaccaacaa gcaatggatg gagtcgcacg acgtaatcct
     5221 gaatatcgtg cgaaacaatt cgacgccgct tgaactggcc gtcaggttcg tcaccaaact
     5281 tacccagaat gactggcgca tgcttgcaag gcccggcgcg gccaggcccg ccatcatgaa
     5341 ccaggcacgc aggcttcttc agggttgtga atagtccccg ccgagcttga cccttttttt
     5401 cctttgcagg aacatcaccc aggctcgctc cggactgaaa tgatgaagtt cccatccgcc
     5461 ctgcggacaa cggtcttgaa gctcttcgtg ctgctggtcg cggccggcat tgccgcaacg
     5521 atgcacctgg ttgtgcttcc cgccccgcag cccgccccga ccgccccgca gccagacggc
     5581 atcagttcgc cgcaactcct tgaagccgcg cgaaaattca ccgggtttat ggtggccagg
     5641 ccatacagct tcaacgatcg catttcggga acgcggcttg ccgacgacag cgatgccacc
     5701 gtgggatatg tgatcgacac cacgtcattg cccggccttg ctaccggcta caacggaaag
     5761 gttccggtcg ccatcgtcct cgacgcggac ggactggttg cgggcatcgc gccgcttgcc
     5821 aacctcgaga ccccatccta catggatatc gttgaatcca gcggcttcct tgacgtcctg
     5881 aaaggtcgga cgattgccca ggcattgacc tcgaccgacg ttgattccgt ttctggcgcc
     5941 acgttcacaa gccaggccat gatttcggcg
//
LOCUS       DAOPPA010000167         5951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14615_length_5951_cov_3.543419, whole genome shotgun sequence.
ACCESSION   DAOPPA010000167 DAOPPA010000000
VERSION     DAOPPA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14615_length_5951_cov_3.543419"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2441
                     /locus_tag="PKH54_06410"
     CDS             <1..2441
                     /locus_tag="PKH54_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99556.1"
                     /translation="NLSLVPPARIEYRVSDNSGLMIPARLTFMPVELDTGDPLDYWTS
                     FNEPELGDSRYDHGIGLTEHSLNGEGTVTVPAGRYNIYVSHGFEYEVKIIRNYEVSSG
                     EKKALSVQINREVDTTGYVAADLHLHTIFSSDSSLPVDMRLKAIMTEGVEYVTANDHD
                     VVINYEPYLADMGLEGYIKTVPSAETSPLEWGHFNLWPMKWDGRDIPLHDPVPWQAGE
                     SMEEIWDAMFSRADGPADAAVMQLNHPRDGTMGYFNQIGFKAHSLQRKNGAMMMCSKA
                     VEDAPCNFHLFEIFNGKNLHYLHTPTIGELERYNACFKEIVAATEKGEFGFEANGRGA
                     VCGWMQADPWPDCGELLEDPMPEGLDEAGIAEWATRHDQCVWHKEMREGFAYCDDDAV
                     TLSNCKRMAMESSKLISGRYMLQRTPQEQTLSVNLYDRAEDDDSVTCNENGCPDVGCS
                     LQEVCEACLKPIAADCFKKGGWNAECMVMCRDNCPQEDSRPCSSSQQPLNDWFALLDA
                     GMNVFASGNSDSHKTSAEVGYPRNLIVSSTDKPHALDRDELNRNLKAGRSIVTSGPMV
                     DFEILAEDTGERAGLGGMVQAKKGKLHARIRVQTPSWFNIDTIEIYRNSMLERQIRLN
                     SRPEDIIDFDDVVELARPDVDSWYVVIVKGLSKDSFMTPVYKRSPYGNILIMTVISLG
                     AQAILANFDSLLSNPDIQSLLDLAGLSLDSALSSTEWPDSIPVVPFATTNPIKVDIDG
                     DGRFMGTRTVDADLDGVPDLPPFCSIPCKTDTDCGDNQLCSPRRDTGEKICRIPASAA
                     CGYDDPPDGELPAE"
     gene            2541..4706
                     /locus_tag="PKH54_06415"
     CDS             2541..4706
                     /locus_tag="PKH54_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOC99557.1"
                     /translation="MMSRIVALVATLLAVSSTASAFEASVVPATRKVVPGDKNTYTTW
                     ATAELAGCRNEWESFQVVIRTEGTLTGVNAAMTDLAGPSDSVIPASAAHLYREWFLKI
                     ENPSGGGISVKNHMREPGLYPDPLIPFVDPYSETPNPVGAPFDIGVNDNGLAVVFVDI
                     HIPDQALPGTYLGTLAVTAEGQDPIAIPVELAVWELDMPNEKNIGTAFRMSDSQVRRY
                     HGGPDEGATSADFDRILKNYYVAAQDHRIDPTGFKGPVDFQFDGDGVLLPVDWTAYDA
                     YMAPYIDGSYFPNGVGVARFDVGMFGPGRGRTMDDEQYKQAAAALANHLDQKGWWHKA
                     WVYSKDEPWLTKPLEDPSVAEQNMMNVANDSRLLVESSDLWKGHILVTGPKFDLAAEW
                     VGIWCPVQPMYDSWFWTEDDYPGRGDYDAHVAGGGELWFYNCNGNMPPYPGYDIDTTI
                     GYEPRILKWGSWYEGATGYLYWRLLYWGDFDPWVEWQDIPGFGPFFARNGDGLLMYPG
                     DGDGTFADARTPEFLSLDGPIVSYRMKQIRDGLEDWEIFIMAEKAGIGDWVKTEIGKV
                     YRRFGNFMELDCERLNYYCPEDEPWTLDEGALLQVRSRIAAKLLFTLYPDRYPDPDEP
                     AIPEVVESGAEITETIEDIAELPGDVGTGDSVTDDTTDSGEAQQDAASDAVQGSDLQE
                     SGRSGCAAGKGSTRDLAAIPALILAAAVAMLMRRKKAAY"
     gene            4880..5224
                     /locus_tag="PKH54_06420"
     CDS             4880..5224
                     /locus_tag="PKH54_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC99558.1"
                     /translation="MPRRRDEDLLKDVGRRLARIRKDRGWTQEQLADKIGIAPVTLSR
                     LETGARAMSLSSLSIIAKQLEVGLGDVLDVKAPAPAQALPPRENEILRLFRECSYDRQ
                     ELLLRVGRELAD"
     gene            5321..>5951
                     /locus_tag="PKH54_06425"
     CDS             5321..>5951
                     /locus_tag="PKH54_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC99559.1"
                     /translation="MAAIAAARSGSVVTVMDHSGGAGTKLLLTGGGRCNITCFLHSAD
                     FVRRLGPKGRFLGHALASMSPGATREFFSLAGVPTTVEADGRVVPVSQRAVDVRRALL
                     EMAATLGVRLQTSVDVTGIKRLDDGWAVTTGSGSHRFDKVILATGGMSYASTGSDGSG
                     YRLATALGHTLTRPVPAEVPLISADLDTSRLQGLSVEPVAISINADRRPR"
BASE COUNT         1276 a         1722 c         1820 g         1133 t
ORIGIN      
        1 tcaacctttc gctggttccg ccggcgcgca tcgagtatcg ggtgtcggac aattccggcc
       61 tgatgattcc ggctcgcctg acgttcatgc cggttgaact ggacacaggg gatcccctgg
      121 attactggac cagcttcaac gagccggaac ttggcgattc caggtacgac cacggcatcg
      181 gcctgaccga acactcgctc aacggcgagg gcacggtcac cgtcccggcc ggccgttaca
      241 acatctatgt gtcccatgga ttcgagtatg aagtgaagat catccggaac tacgaagtgt
      301 cttccggcga gaagaaggca ctttcggttc aaatcaaccg ggaagtcgat acgaccggtt
      361 acgtggcggc cgaccttcac cttcacacca tcttctcgtc cgattcgtcg ctgccggtag
      421 atatgcgcct gaaggccatc atgaccgagg gtgtcgaata cgtgacggcc aacgaccacg
      481 acgtggtcat caattacgaa ccgtatcttg ccgacatggg tctggaggga tacatcaaga
      541 cggttcccag cgccgaaacg tccccgctcg agtggggaca tttcaacctg tggccgatga
      601 aatgggacgg ccgcgacatc ccgcttcacg atcctgttcc gtggcaggcc ggcgaatcga
      661 tggaggaaat ctgggacgcc atgttctccc gggcggacgg gccggcggac gccgcggtca
      721 tgcagcttaa ccacccgcgc gacggaacga tgggctactt caaccagatc gggttcaagg
      781 cccactcgct gcagcgcaag aacggcgcga tgatgatgtg ttccaaggcg gtcgaggacg
      841 ctccgtgcaa cttccacctg ttcgagatct tcaacggtaa gaaccttcat tacctgcaca
      901 cgccgaccat cggcgaactg gaaaggtaca acgcgtgctt caaggagatc gtcgcggcga
      961 cggaaaaggg cgaattcggc ttcgaagcga acgggcgtgg cgccgtctgc ggctggatgc
     1021 aggccgatcc gtggccggac tgcggcgaac ttcttgagga tcccatgccg gaaggcctgg
     1081 acgaagcggg aatcgccgag tgggcgacgc gccatgacca gtgcgtctgg cacaaggaga
     1141 tgcgcgaggg attcgcgtac tgtgacgacg atgccgtcac gctttcgaac tgcaagcgca
     1201 tggcgatgga atcgtcgaag ctgatatccg gaaggtacat gctgcagcgc acgccacagg
     1261 aacagaccct ttccgtgaac ctgtacgacc gcgccgaaga cgacgattcc gtgacgtgca
     1321 acgaaaacgg ctgtcccgat gtcggatgca gcctgcagga agtatgcgaa gcctgcctga
     1381 agccaatcgc cgccgactgt ttcaagaagg gcggctggaa cgccgaatgc atggtgatgt
     1441 gcagggacaa ctgcccgcag gaggactcaa ggccgtgcag cagttcccag cagccgctga
     1501 acgactggtt cgccctgctt gacgccggca tgaacgtgtt cgcaagcggc aactcggact
     1561 cgcacaagac gtcggccgag gtcgggtatc caaggaactt gatcgtttca tccaccgaca
     1621 agccgcacgc gcttgatcgg gatgaactga accgcaacct gaaggccggg cgctcgatcg
     1681 tcacatccgg accgatggtt gattttgaaa tcctggctga agacaccggc gaacgtgccg
     1741 gcctgggcgg catggttcag gcgaagaaag gcaagctgca cgcgcggatc agggttcaga
     1801 cgccgtcgtg gttcaacatc gacaccatcg aaatttaccg taactcgatg ctcgaacgac
     1861 agattcgcct caactcgagg ccggaagaca tcatcgactt cgacgacgtg gtggaactcg
     1921 cccgtccgga cgtcgattcg tggtacgtcg ttatcgtgaa gggcctgtcg aaggactcgt
     1981 tcatgacgcc ggtttacaag cgaagcccct acgggaacat ccttatcatg acggtcatct
     2041 cgcttggcgc ccaggcgatc ctcgccaact tcgactcgct gctgagcaat ccggacatcc
     2101 agtcgctgct ggatcttgcc gggctttcgc tggacagcgc gctgagttcc accgaatggc
     2161 ctgacagcat cccggtcgtc ccgttcgcga caaccaaccc gatcaaggtt gatatcgacg
     2221 gcgacggaag gttcatggga acgaggacgg tcgatgccga tctggatgga gtcccggacc
     2281 tgccgccgtt ctgctcgata ccgtgcaaga ccgataccga ttgtggcgat aaccagttgt
     2341 gttcgcccag gcgcgacacc ggcgaaaaga tctgccgcat cccggcaagc gcggcatgtg
     2401 gatacgacga cccgcctgac ggggaactcc cggccgaatg acgcggcccc tttcgcgggt
     2461 caactaatcg cagcccgtaa ccattgatta tccggccacg aaccgctatg ctgcggcgcg
     2521 ttatctgatg gagcctgtcc atgatgtcaa gaatcgtcgc actggtcgcc accctcctgg
     2581 cggtgtcttc aacagcttcg gcttttgagg ccagcgtggt gccggcaaca aggaaggttg
     2641 tgcccggcga caagaacact tacaccacat gggcgaccgc cgaattggcg gggtgccgga
     2701 acgaatggga atccttccag gtggtgatcc ggacggaagg cacgctgacc ggcgtcaacg
     2761 ccgcaatgac cgatctcgcg ggtccctcgg acagcgtgat cccggcttcg gccgcgcatc
     2821 tctacaggga atggttcctg aagatcgaaa acccctcggg gggcggcata tcggtcaaga
     2881 atcacatgcg cgaaccaggc ctttatcccg acccgctcat cccgtttgtg gatccgtatt
     2941 ccgaaacgcc gaatccggtc ggcgctcctt ttgacatcgg ggtaaacgac aatggactgg
     3001 cggtcgtttt cgtcgatatc cacataccgg atcaggcatt gccgggaacc taccttggca
     3061 cgctggcggt taccgccgaa ggccaggatc cgatcgccat cccggtcgaa ctggctgtct
     3121 gggaacttga catgccgaac gagaagaaca tcggaaccgc gtttcgcatg tccgacagcc
     3181 aggttcgcag atatcacggc ggacccgatg aaggcgcgac atcggccgac ttcgaccgca
     3241 tcctgaaaaa ctactacgtg gccgcgcagg atcacaggat cgacccgacc ggtttcaagg
     3301 gcccggtgga tttccagttc gacggcgacg gcgtgctgct gcccgtggac tggacagcct
     3361 acgatgcgta tatggcaccc tacatcgacg gatcgtactt cccgaatgga gttggagtcg
     3421 cgcgtttcga cgtgggcatg ttcggccccg gccgcggacg gacgatggac gacgaacagt
     3481 acaaacaggc tgccgcagcc cttgcgaatc acctcgacca gaagggctgg tggcataagg
     3541 cgtgggtata ttccaaggac gaaccgtggc ttacgaaacc gctcgaggat ccctcggtgg
     3601 cggaacagaa catgatgaac gtcgccaacg attcaaggct gctggtggaa tcttccgacc
     3661 tttggaaggg acacatcctt gtaaccgggc cgaaattcga tcttgccgcc gaatgggtcg
     3721 gaatctggtg tccagtccag cccatgtacg acagttggtt ctggacagag gatgattacc
     3781 cgggacgtgg cgattacgac gcgcacgtgg ccggcggcgg cgagctctgg ttttacaact
     3841 gcaacggcaa catgccgcca taccccggtt acgacatcga cacgactatc ggatacgaac
     3901 cccggatcct gaagtggggt tcctggtacg agggcgcgac cggatacctt tactggcgac
     3961 tgctttactg gggcgatttc gacccgtggg tcgaatggca ggacatcccg ggattcggcc
     4021 cattcttcgc gcgaaacggc gacgggctgc tgatgtaccc gggtgatggc gatggaactt
     4081 tcgccgatgc ccgcacgccc gaatttctgt ccctggacgg ccccatcgtc agttaccgca
     4141 tgaagcagat acgggatggc ctggaagact gggaaatttt catcatggcc gaaaaggccg
     4201 gtatcggcga ctgggtgaag acggaaatcg gcaaggtcta tcgccgtttc ggcaacttca
     4261 tggaactgga ctgcgaacgc ctgaattatt actgtcccga agatgaaccg tggacactgg
     4321 atgaaggtgc gcttcttcag gttcggtcgc gcatcgccgc caagctgctg ttcacgcttt
     4381 accccgacag atacccggat ccggatgaac cagccattcc cgaagtggtg gagtcaggcg
     4441 cggaaatcac cgaaacaatt gaagatatcg ctgaattacc tggcgatgtg ggaacgggcg
     4501 acagcgtgac cgacgacacg acggacagcg gcgaagccca acaggacgcg gcgtccgatg
     4561 cggttcaggg atcggatctg caggaaagcg gtagatccgg atgcgccgcc ggcaagggat
     4621 cgacacgcga tctggcagcg attcctgcat tgatattggc tgccgctgtc gcgatgttga
     4681 tgcgacgcaa aaaggcggca tactgatttt ttcaaaaaaa atcaaaagtt cagacatttg
     4741 ggcttttata tttgatttga tgcagatata taattcgcga tgctggtaac gacagtcggt
     4801 gacgccaaat tcaacttgcc tggaatgacg gacgacgact gcctgttgta ttggaaatga
     4861 aaagcactgg aggtgcagtg tgccaagaag aagagatgaa gacctgttga aggatgttgg
     4921 ccgtcggctg gccaggatcc gcaaggatcg cggctggacc caggaacagc ttgcggacaa
     4981 gatcgggatt gccccggtca cgctgtccag gctggaaacc ggcgcccgcg caatgagtct
     5041 gtcttccctg tcaatcatcg caaagcagtt ggaagtcgga cttggcgacg ttctggacgt
     5101 caaggctccc gcacccgcgc aggcgcttcc gccgcgcgaa aacgagatcc ttcgcctgtt
     5161 ccgtgaatgc tcgtacgatc ggcaggaact gctgcttcgt gtcggcaggg aactggctga
     5221 ctagcgccag gtacatcctg atgacataat ctgccgatct ctcggcggaa tgaagatgcc
     5281 cggaacgatt tgtgtaactg ggggcgggcc ggcaggcctg atggcagcca ttgcggcagc
     5341 caggtcagga tcagtcgtca ccgtaatgga tcattcgggc ggcgccggaa caaaactgct
     5401 gcttaccggt ggcgggcgct gcaacatcac ctgctttctt cacagtgctg atttcgtacg
     5461 gcgccttggg ccgaaaggcc gattccttgg tcatgccctg gcctcgatgt cccccggggc
     5521 gacaagggaa ttcttcagcc tggccggcgt tccaacaact gtcgaggccg acggacgcgt
     5581 cgtgccggtt tcccagcgcg ccgttgacgt aaggcgagcg ctgctggaaa tggccgcgac
     5641 gctcggcgtc aggttgcaga cgtcggttga cgtaacagga ataaagcgac tggacgacgg
     5701 atgggcggtc acgacgggat cgggcagcca ccggtttgac aaggtcatcc tggcaaccgg
     5761 tggaatgtct tatgcatcga ccggttctga cggttccggc taccggctgg cgaccgcgct
     5821 tggccacact ctgaccaggc ctgttccggc cgaagtcccc cttatttccg ccgatctgga
     5881 cacatcccgc ctgcagggac tttcggttga accggtggcc atttccataa acgcggatcg
     5941 gcggccgcgc a
//
LOCUS       DAOPPA010000168         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14617_length_5950_cov_4.103138, whole genome shotgun sequence.
ACCESSION   DAOPPA010000168 DAOPPA010000000
VERSION     DAOPPA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14617_length_5950_cov_4.103138"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /gene="lspA"
                     /locus_tag="PKH54_06430"
     CDS             <1..643
                     /gene="lspA"
                     /locus_tag="PKH54_06430"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOC99560.1"
                     /translation="KRLVSRAAPESDPATVAKQVQQELSSVTVRDFILERIPAESKRT
                     VDRTIREGLFVVPRTGLVQVEPDSPVEPGATYLLAERTVTVVEGFWEYSYAENPAGAF
                     SMLLWLDAKLRLAIFLTFGIVAILALGYMIVRPPSESWLLTVALGAVLGGAIGNYGDR
                     VTLTYVVDFIHMYWRDYHWPKYNIADIGITGGVIVLLAFSIFWPNGSKKSRSA"
     gene            662..1507
                     /gene="speE"
                     /locus_tag="PKH54_06435"
     CDS             662..1507
                     /gene="speE"
                     /locus_tag="PKH54_06435"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOC99561.1"
                     /translation="MSERRFIERDPYAPIQYGYQVDQVLCSGRSDFQEYQVITNPFFG
                     RIMILDGVVQLTERDEFFYHEMLAHVPLHSRQQPENVIVIGGGDGGAVREVVRHDCVK
                     NVWLVDIDPEVTRLSKEYFPAVACGLSDPRVRVLHEDGAAFLRNFDGRADLVVVDSTD
                     IVGMAKVLFSPQFFGDVNRVLTDDGLFVTLSESLHFHFPIVRDVQNTMRDVFGHADLY
                     TAPIATYGGNWWCFAVGHKGRGCRAKVRPTVAGTRYYCDEIHSGCFLPDFLYSRLMDP
                     SRDRF"
     gene            1587..2567
                     /gene="holB"
                     /locus_tag="PKH54_06440"
     CDS             1587..2567
                     /gene="holB"
                     /locus_tag="PKH54_06440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOC99562.1"
                     /translation="MKFSSVIGNEGVARVLRRAVDTGRVAPTILFHGRDGVGKKTMAM
                     AFVSYLLCRDRRDGDSCGICPNCRSMDEGRYVDLEILEPDKGVIKIDAVRDAMPRLQY
                     QPVIGPWKVLVIDDAHAMNTEAANAALKSLEEPPAGTLFILITSSPDTLPRTVLSRSF
                     QIPFGPVPTSAIIELLVSARGIEPDVAACAAAMCSGCPGQAFRLLDSQALAERRDFIK
                     AFLSLVDQPDRVRLQFSDGVPVDRDGGDVYRLILESVARDLLAVVSGVSLDDLSNGDM
                     KDELVAFAHKAGPDRVLAIAEAWLDWDAARAYYPAPKNAIDRMVLGMPQR"
     gene            complement(2586..>5950)
                     /locus_tag="PKH54_06445"
     CDS             complement(2586..>5950)
                     /locus_tag="PKH54_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99563.1"
                     /translation="SCDADGNGCTAGDKCVSGNCRAGTAPDCSAAGDQCNVGQCVSTG
                     SATYDCTPNPAPRNGSSCNADNDGCTAGDTCADGTCVAGTAPDCGVASDQCNVGFCSS
                     LGPQTFECRKNPVPLDGAGCNADGNGCTVGDMCQAGTCTAGPAPDCSSLDGPCANGIC
                     QSTGAASFRCTIDWTPFQDMACNADNSGCTIADKCRFGTCVAGPAADCSAFADQCHAG
                     ACDPDGPDGFTCRADAAAKEGARCNADGNGCTVGDTCVSGTCTVGPDADCTVASDACN
                     AGSCWSSGPNSFICEKDPAPREGLPCDADGDGCTVGDACVSGSCAAGSRPDCSSESDQ
                     CNTGICKSTSNMAYVCIKDPAGYNGRTCDADDNGCTTSDYCQSGSCLVGNPVDCGWAD
                     DQCNIGYCQSAGPYDHECLLDPLPLHGLACNADDDGCTLSDRCDAGACVAGLPADCSE
                     LDAPCSVGICESTGVFSFACVADFEPFQGEPCNADDDGCTMSDACILGECVEGQTADC
                     GEMDDQCNAGYCLSGGPDSFSCQKDPRPFEGLSCNADDDGCTAGDSCLDGACIAGDTP
                     DCSGETDQCNVGTCVAAGADDYFCMADPSPLEGHSCDADTDGCTIGDFCGDGTCHEGP
                     EADCTMQDDQCNTGICESTGSDSFECVSNPIPHEGAACDSDGSGCTVGDACEGGVCAA
                     GDVPDCSSMTDQCNAGECRSTGTDSFVCVKNPEPRENTLCNADSNGCTEGDRCVAGAC
                     TPGAPPDCSAVENQCTASICQSRGPTTHACLADTQTLSGTACDDGDQCSTDDKCQVGS
                     CVGSPVVCADEFTCTTNRCDANTGACTATTDDAACDDSYPCTDDTCSALSGCENVNIP
                     DWSECSGTDYACFAGRCEPTGPGDRCPEPIVLESGVPIRVNAGMLHQYLVVESSCMDP
                     APSGIDAFLSFVADAGHTFTLTIEPDQGVTVAVVFRESCQADSTCAAGAPPSSTASTI
                     DLPQDASGEILVQVIFTDCGGQCLRKGAVLTITDTTPAEPAPETSEDTVQPDAMAEIG
                     LDAASTDLAESDSTRPDAGEPQDTGIDMSGSDFLSLDANDRTDDGGGTGGGGCSSSGG
                     SHEPIAVLFFLVGFFMIMSGAAKRRRTNEKTR"
BASE COUNT          986 a         1851 c         1922 g         1191 t
ORIGIN      
        1 gaagcgtctg gtttcgcggg cggccccgga atccgatccg gcgaccgtcg cgaagcaggt
       61 tcagcaggaa ctgtcgtcgg taacggtgcg cgatttcatc cttgagcgga tcccggccga
      121 atcgaagcgc accgttgacc gcaccatccg ggaaggcctg ttcgtggtgc ccaggaccgg
      181 gctggtgcag gtggaacctg actccccggt cgagcccggt gcgacgtacc tgctggccga
      241 acggaccgtc accgttgtcg aagggttctg ggagtattct tacgccgaga accctgccgg
      301 ggcgttctcg atgcttttgt ggcttgacgc aaagctgcgc ctggccatct tcctgacttt
      361 cgggattgtg gcaatcctgg cgcttggtta catgatcgtc cggccgcctt cggaaagctg
      421 gttgctgacg gttgctctgg gtgcggtgct cggcggcgcg atcggcaatt acggcgaccg
      481 cgtgacgctt acgtatgtcg tcgatttcat ccacatgtac tggcgcgact atcactggcc
      541 gaaatacaac atcgccgata tcggcatcac tggcggcgtg atcgtccttc tggccttttc
      601 cattttctgg ccgaacggtt caaagaagtc ccgttccgcc tgatcatccg caggtgtgcc
      661 catgtcggaa cgaaggttta tcgaaaggga tccttacgcc ccgattcagt acgggtacca
      721 ggttgatcag gtcctgtgca gcggacgttc cgatttccag gaataccagg tcatcacgaa
      781 tcccttcttc gggcgcatca tgatccttga cggggtcgtc cagcttaccg aaagggacga
      841 attcttctat cacgagatgc tcgcccacgt gccgctgcat tccaggcagc agcccgaaaa
      901 cgtgatcgtg attggcggcg gcgacggcgg ggcggtccgc gaggtcgtgc ggcacgactg
      961 cgtgaagaat gtgtggctgg ttgacatcga tccggaagtc accaggcttt cgaaggagta
     1021 cttcccggcg gtagcgtgcg ggctgtccga cccaagggtc cgggtgctgc acgaggacgg
     1081 ggccgcgttc ctgagaaatt tcgacgggcg cgccgatctg gtggtcgttg acagcaccga
     1141 catcgtcggc atggcgaagg tgctgttttc cccgcagttt ttcggtgacg tcaatcgggt
     1201 gctgactgat gacggacttt tcgtgacgct ttcggaatcg cttcatttcc actttcccat
     1261 cgtgcgggat gtccagaaca cgatgcgcga cgtctttggt cacgccgacc tttacaccgc
     1321 cccgattgcg acctacggcg gcaactggtg gtgcttcgcc gtcggccaca agggccgggg
     1381 ctgtcgcgcg aaggtgcgcc cgaccgttgc cggaacaagg tattactgcg acgaaatcca
     1441 ctccggatgc ttcctcccgg attttcttta cagccggctg atggatccat cccgcgatcg
     1501 cttctgaatt tcggcacgcc cactaccgga ttgactgatg ccgccgcagc gattaccgtt
     1561 gcgatggttg ttcccccggg gtttcgatga agttttcttc cgtaatcggg aatgagggcg
     1621 tggcgcgcgt gctgcggcgc gccgtcgata ccgggcgcgt ggccccgacc attctctttc
     1681 atggccgtga cggcgtcggc aagaagacga tggccatggc gttcgtttca tacctgctgt
     1741 gcagggatcg tcgcgacggc gattcgtgcg gaatctgtcc gaactgccgc agcatggacg
     1801 aaggaaggta cgtcgatctt gaaatccttg aacccgacaa gggcgtcatc aagattgacg
     1861 cggttcggga cgcgatgcca cgcctgcagt accagccggt catcggtcca tggaaggtgc
     1921 ttgtcatcga cgatgcccac gccatgaaca ccgaggcggc gaatgccgcg ctgaagtcgc
     1981 tggaggaacc gccggccgga acgctgttca tcctgatcac atcaagtccc gacacattgc
     2041 cgcgaacggt tctttcgcga agcttccaga tcccgttcgg gccggttcca acttcggcaa
     2101 tcatcgaact gctggtgtcg gcgcgaggga tcgagcccga cgtggccgcc tgtgccgcag
     2161 cgatgtgttc cgggtgtcct ggccaggcct tccggctgct cgacagccag gcgctggcag
     2221 aaaggcggga tttcatcaag gcattccttt cgctggtgga tcagcccgac agggtcaggc
     2281 tgcagttttc cgacggcgtt cctgtcgatc gcgacggtgg cgacgtgtat cgccttatcc
     2341 ttgaatcggt cgcgcgtgac ctgcttgcgg tcgtttccgg ggtttccctg gacgatcttt
     2401 caaacggcga catgaaggat gaacttgtcg cattcgcgca caaggccggt cccgacaggg
     2461 ttcttgccat tgccgaagcc tggctcgact gggatgccgc cagggcgtac tacccggccc
     2521 cgaaaaacgc catcgacaga atggtgcttg gcatgccgca gcgctgaccg cgtccagggg
     2581 tcacgtcatc gcgtcttttc gttcgtccgt cttcgtttcg cggcgcccga catgatcatg
     2641 aagaagccga caaggaagaa cagaactgca atcggttcgt gtgacccgcc cgagcttgaa
     2701 cacccgccac cgcccgttcc gccgccgtcg tcggttctgt cgtttgcatc gagcgacaaa
     2761 aagtcgcttc ccgacatatc gatgcccgtg tcctgcggtt ccccggcgtc cggccgggtc
     2821 gagtcggatt ccgcgagatc cgtggatgcc gcgtccaggc cgatttccgc catggcatcc
     2881 ggctgcacgg tatcttcgga agtctcggga gccggttcgg ccggggtcgt gtccgttatc
     2941 gtcaggacgg cgcccttgcg caggcactgg ccgccgcagt cggtgaagat gacctggaca
     3001 aggatttccc cggacgcatc ctgcggcagg tcgatggtcg atgcggtcga agacggtggc
     3061 gcgcccgcgg cgcaagtcga gtcggcctgg catgactcac ggaataccac ggcgaccgtg
     3121 acaccctggt ccggttcaat cgtcagcgtg aacgtgtgtc cggcgtccgc gacaaacgac
     3181 aggaatgcgt cgatgcccga tggcgcagga tccatgcagg agctttcgac aacgaggtac
     3241 tggtggagca tgccggcgtt gacgcgaatc ggaactcccg attccagcac gatcggttcg
     3301 gggcacctgt cgccagggcc ggtcggttcg cagcgtccgg cgaaacacgc gtaatccgtc
     3361 ccgctgcatt cgctccagtc cgggatgttg acgttttcgc aaccggacag ggcggaacac
     3421 gtgtcgtcgg tgcaggggta cgagtcgtcg caggctgcgt cgtccgtggt tgccgtgcag
     3481 gcccccgtat tcgcgtcgca ccggttcgtg gtgcatgtga attcatcggc gcagacgacc
     3541 ggcgagccga cgcacgaacc gacctggcac ttgtcgtcgg tcgagcactg atcaccgtca
     3601 tcgcaggcgg ttcccgaaag cgtctgggtg tccgcaaggc aggcgtgggt tgtgggcccc
     3661 ctggactggc agattgaggc ggtgcactgg ttttcaaccg ccgagcagtc cggcggcgca
     3721 cccggcgtgc aggcgccggc aacgcatcgg tcaccttccg tgcacccgtt gctgtcagcg
     3781 ttgcagaggg tattttccct tggttccggg ttcttgacgc agacaaaaga atcggtgccg
     3841 gttgatctgc attcgccggc gttgcactga tcggtcatgg aagagcagtc cggaacgtcc
     3901 ccggcggcgc acacgccgcc ttcgcaggcg tcgccgaccg tgcatccgct tccgtccgag
     3961 tcgcacgccg cgccttcgtg ggggatcggg ttggatacgc actcgaacga atcggagccg
     4021 gtcgattcgc atatgccggt gttgcactgg tcgtcctgca tggtgcagtc ggcttccggg
     4081 ccttcatggc aggttccatc cccgcagaag tcgccgatgg tgcaaccgtc ggtgtcggca
     4141 tcgcacgaat gaccttcaag tggcgaggga tcggccatgc agaagtaatc gtccgcaccg
     4201 gctgcgacac aggtgccgac gttgcactga tctgtctcgc ccgagcagtc cggcgtgtcc
     4261 ccagcgatgc acgcgccgtc caggcacgaa tcgccggccg tgcatccgtc gtcgtccgcg
     4321 ttgcaggaaa ggccctcgaa gggccgaggg tccttctgac aggagaagct gtcggggccg
     4381 ccggacaggc agtaccccgc gttgcactgg tcgtccattt cgccacaatc agccgtctgt
     4441 ccttcgacgc attcgcccag gatgcatgcg tccgacatgg tgcagccgtc gtcgtcggcg
     4501 ttgcatggct cgccctggaa cggttcgaag tcggccacgc aggcgaatga gaacaccccg
     4561 gtcgattcac agattccaac cgagcaggga gcgtccagct ccgaacagtc ggccggcagg
     4621 ccggccacgc aggccccggc gtcgcaacgg tcggaaaggg tgcatccgtc gtcgtcggcg
     4681 ttgcatgcaa gtccgtgaag cggcaacggg tcaagcaggc actcgtggtc gtaaggaccg
     4741 gcggactggc agtaaccgat gttgcactgg tcgtcggccc agccgcagtc aacaggattg
     4801 ccgacgaggc agctcccgga ctggcagtag tcggaagtgg tgcatccgtt gtcgtcggca
     4861 tcgcacgtcc ggccgttgta tccggcggga tccttgatgc agacataggc catgttcgac
     4921 gtggatttgc agattcccgt gttgcactgg tcggattcgg acgaacagtc cggtcgggaa
     4981 ccggccgcgc acgaacctga aacacaggcg tccccgaccg tgcatccgtc gccgtcggcg
     5041 tcgcacggaa ggccttccct gggtgcggga tccttttcgc aaatgaagct gttcggtccg
     5101 ctggaccagc aggaaccggc gttgcatgcg tccgacgcga ccgtgcagtc ggcgtccggg
     5161 cccaccgtgc aggtgccgga aacacaggtg tcgccaaccg tgcagccgtt gccgtccgcg
     5221 ttgcagcgcg cgccttcctt tgccgcggcg tcggcccggc atgtaaagcc gtctggcccg
     5281 tcagggtcgc aggcgcccgc atggcactga tcggcaaatg ccgagcaatc cgcggcgggg
     5341 ccggcgacgc atgtcccgaa ccggcatttg tccgcgatgg tgcaaccgct gttgtcggcg
     5401 ttgcacgcca tgtcctggaa cggagtccag tcaatcgtgc atctgaagct ggccgccccc
     5461 gttgactggc agattccgtt ggcgcaaggg ccgtcaaggc tgctgcagtc cggtgcggga
     5521 ccggcggtgc atgttccggc ctggcacatg tctccgaccg tgcagccgtt gccgtcggca
     5581 ttgcagccgg cgccgtcaag cggcactgga ttcttcctgc attcgaacgt ctgcgggccc
     5641 agggacgaac agaatccgac gttgcactgg tcgcttgcca ccccgcagtc gggcgcggtt
     5701 cccgcgacgc atgttccgtc cgcgcacgtg tccccggcgg tgcagccgtc gttgtcggcg
     5761 ttgcaggatg aaccgttgcg cggcgccggg ttcggcgtgc agtcataagt tgcggaaccg
     5821 gtgcttacgc actgtccgac gttgcactgg tcgcctgccg cgctgcagtc cggtgccgtt
     5881 ccagcccggc agttgcccga tacacacttg tccccggccg tgcacccgtt gccgtcggca
     5941 tcgcatgacg
//
LOCUS       DAOPPA010000169         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14632_length_5945_cov_3.610526, whole genome shotgun sequence.
ACCESSION   DAOPPA010000169 DAOPPA010000000
VERSION     DAOPPA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14632_length_5945_cov_3.610526"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKH54_06450"
     CDS             complement(<1..337)
                     /locus_tag="PKH54_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC99564.1"
                     /translation="MSSCHHDNGPARLRGNAGLTIVELLVALSIASIIMFGIYSVYSV
                     SADTYRIQNEIMEAMDQARFGLEQVKRDISKAAFLGTPNSVGDAMVCPKPGNPIRGII
                     VERSGAVVNP"
     gene            complement(318..947)
                     /locus_tag="PKH54_06455"
     CDS             complement(318..947)
                     /locus_tag="PKH54_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOC99565.1"
                     /translation="MKVNRPARTASAKGFTLMELVVVMAITVTGFVAALYLQSSLMKG
                     ASNSWDTTCAVQLARHTLETIRLEAVEWYNDTGAGVGGVQQLKFAYLRNVGAATPGGT
                     SGWQRAPYANSALPFQLTNQLGDNVGWDEGANDEITNAVARRYSVQYRLTWIVPNMLI
                     RAETRVMWPRTDMAAGNYDTCPADMFNHPNDIMSVTIPMTVMKNVFVSP"
     gene            complement(957..1568)
                     /locus_tag="PKH54_06460"
     CDS             complement(957..1568)
                     /locus_tag="PKH54_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC99566.1"
                     /translation="MSRTDSRRGFTFIEMMMVVAVMTSLAAVTLPAVMQGYSRQAARN
                     AAVDVQGIIDFARTQASMSNIAHLVVPILATGPAFTGQIEVWQGISSACQSFRTPDGG
                     TGQQGVRIRVLDLHDQFPEVRMVSTHPANLPTVPLCFKPDGRVFQLIGDVTPQIIPAV
                     MGSGMAGGTAAFRLRKFDEGGNAEGPIHSVIVPFNGISRKVVE"
     gene            complement(1684..3735)
                     /locus_tag="PKH54_06465"
     CDS             complement(1684..3735)
                     /locus_tag="PKH54_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HOC99567.1"
                     /translation="MKIPFPAGIKRFTPTFVRLPATLAMMLMLASSATTAAEPDQNPA
                     EPVAAEQAAATDEPATTPPEAAADEPATEPASETGKAPDSNDDSTGAANDAEDRPTDE
                     KAAARTSDRARADDPVARAEAAKIREEALGGIRNRPLSPAGYPKIEHDGYFRLRMDGF
                     YRPHLGTTAYDNRTQKAYNSSAYPSPITENYINKDRLSQEDWLSSMNIRFRWIPTIHV
                     MKNIAIVAQFDVLDNLILGSTPDSDADRPDAPLSIFARSQAVPDESVKVKQAYMIWSP
                     FNPDNTKDFLLSISAGRMARHWGLGTMDNDGGDIDADFGTYVDRVNVLARYWNVYLEL
                     GFGWTATGATSATDIQAYGQPYDLTNSDNVFDFTASIFQKPLTMAGRLQRADRLVSGK
                     PVFDWGVYVRYRWQKTDSKQKMDDALAVTPEADYDGYELEERDAWTVSPNLWMKFEWK
                     HSSKERLRLEVEANGMFGHIGRTPRFRFDSTLDQWLEDGHQSLDIRSWAVAFEGEYQW
                     GPVNVGLLVGAASGTDADYWGWRDRNNVATNKDLKTLSSHYFNPDYHVDELLFRQVIG
                     TVTNSFYVKPWFMYDFFESRKDALALGLGILYGQAIETRATPGNDAPLGLEIDVNLLY
                     ENAGVFYVGVTWATLVPFDGLDLATDYGCTALNQNGRCQDSRIDSSWAMSLRGVFGVR
                     F"
     gene            3911..4519
                     /locus_tag="PKH54_06470"
     CDS             3911..4519
                     /locus_tag="PKH54_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99568.1"
                     /translation="MKSIAVASFVTSSILFACSAMAQDALPEPAAECVPKAEAPVAPV
                     DNRPIINKKSLKMRGYDVIEQVELAQFGPGWSAVLLKLALPEAENEALAEVSRLYILE
                     GDRIEFDSFAFDAIEDGIEPSVSTRTFFRMKWEVTMKGKSPSGLILTGIVPRQLPGDK
                     IRVATRTLVLTWNKASGFREVADVVSREPARVNGGIMTVTVN"
     gene            4537..5907
                     /locus_tag="PKH54_06475"
     CDS             4537..5907
                     /locus_tag="PKH54_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC99569.1"
                     /translation="MTENSQPEGILSGLNPEQFAAATHESGPLMILAGAGSGKTRTIT
                     HRIAWLIGNRAVHPSSIMAVTFTNKAAQEMRDRVRALVGSDATPGWMGTFHSVCLRLL
                     RIHGARLGFKPDFVVYDDDDSERLLKLVLDSMGIPRDGVRQYSNAIDRLKNDGVVHPP
                     EAGTLRERAIADIFKRYQAELFKAGAMDFGDLLVMAARLLRENDDVRESLQARFSHLI
                     VDEFQDTNLVQYDFLKLLCRPDPDICVVGDDDQSIYSWRGARVSNILDFATDFPGVTK
                     VVLGRNYRSSANVLGAASGVIRFNRDRYPKDLQAVRGIGEKVYVKQLLNEAGEAAFVV
                     DELASRHRRGFALSRMAVFYRTNAQSRVFEDTLRRRKIPYRVIGGMRFYHRAEIKDII
                     SYLRLLVNPMDSVALDRVVNVPARGIGKTTMDKVRAHSDLEGIPLMQALVEVGDQSGK
                     AVRARI"
BASE COUNT         1148 a         1741 c         1800 g         1256 t
ORIGIN      
        1 ccgggttaac aacggcaccg gatcgctcga cgatgattcc acggattgga ttgcccggct
       61 tggggcacac catcgcgtcg ccaaccgagt tcggcgtccc caggaaggcc gcctttgaaa
      121 tgtcccgctt gacctgttcc aggccaaaac gggcctgatc catggcctcc atgatttcgt
      181 tctggatcct gtaagtatcg gccgaaaccg aatagaccga ataaatcccg aacattatga
      241 tgctggcgat ggaaagggcc accagcagtt cgacaatcgt cagtccagcg ttcccccgaa
      301 ggcgggcggg gccgttatca tggtgacacg aagacatttt tcatcaccgt catgggaatt
      361 gtcaccgaca tgatgtcgtt cgggtggttg aacatatcgg cggggcaggt atcgtagttg
      421 ccggccgcca tgtcagtcct tggccacatg accctggttt cggcgcggat cagcatgttg
      481 ggcacgatcc acgtcaatcg atactgcacg ctatagcgtc gggccacggc gttggtgatt
      541 tcatcgttgg cgccttcgtc ccagccgacg ttgtcgccaa gctggtttgt aagctggaag
      601 ggcaatgccg aattcgcgta cggcgccctc tgccatccgg atgtgccgcc cggggtcgcc
      661 gcgccaacat tgcgaagata ggcgaatttc aactgctgga cgccaccgac gccggcgccg
      721 gtatcgttgt accattcgac ggcttccagg cggattgttt cgagcgtgtg gcgggcaagc
      781 tgtacggcgc acgtcgtgtc ccacgaattg gaagcgccct tcatcagaga agactgcaaa
      841 taaagcgccg ccacgaatcc ggtcacggta atggccatga ccacgaccag ttccatcagc
      901 gtgaaacctt ttgccgaagc cgttcttgcc gggcggttga ccttcatctg cattacctat
      961 tccacgacct ttctgctgat gccgttgaac ggaacgatga ccgaatgtat cggcccctcg
     1021 gcgtttccac cctcgtcgaa ctttcggagc ctgaaagccg cggttccgcc ggccatgccg
     1081 cttcccataa ctgccggaat gatctgggga gtgacgtcgc caatcaactg gaacaccctc
     1141 ccgtcaggct tgaaacacag gggaaccgtc gggaggttgg ccgggtgcgt ggaaaccatc
     1201 cgaacctcgg ggaactggtc atgaagatcc agaacccgga tcctgacgcc ctgctggccg
     1261 gtgcccccgt ccggagtcct gaagctctgg catgccgatg aaatcccctg ccagacttca
     1321 atctggccgg taaatgccgg accggtcgcc agtatcggca ccaccaggtg ggcgatgttg
     1381 ctcatcgatg cctgggtgcg ggcgaagtcg atgatgccct ggacatcgac cgcggcgttc
     1441 cttgcagcct gcctgctgta tccctgcatg actgccggaa gcgtcaccgc tgcaagcgac
     1501 gtcatgacgg caacgaccat catcatttcg atgaacgtga agccacgtct tgagtccgtc
     1561 ctggacatcg ttcctccggc ccgggaaggg aatcgtcagg caaacgtacg acgatgcatt
     1621 gtagcataag gcggattccc aacccaaatg cgatgaaagg gccgtcttga aatggtttga
     1681 ggatcagaag cgcacgccga acacgccgcg cagggacatg gcccaggagg aatcgattct
     1741 ggagtcctgg caacggccgt tctggttcaa tgcggtgcat ccgtaatcgg tcgccaggtc
     1801 cagtccgtcg aacgggacga gggtggccca cgtcacgccg acatagaaca ccccggcatt
     1861 ttcgtacagc aggttgacgt cgatctcaag gcccagcggg gcatcgtttc cgggggtcgc
     1921 cctggtctcg atggcctgcc cgtacaggat gccaaggcca agggcgagcg cgtccttgcg
     1981 gctttcgaag aagtcgtaca tgaaccaggg cttcacatag aacgagtttg taacggttcc
     2041 gataacctgt cggaacagca gttcgtcaac gtggtaatcg gggttgaagt agtgcgagct
     2101 taaagtcttc aaatccttgt tcgtggcgac gttgttccgg tcgcgccatc cccagtagtc
     2161 ggcgtccgtg ccgctggccg cgcccaccag cagtccgacg ttgaccggac cccactggta
     2221 ttcgccctcg aacgccaccg cccaggagcg gatatccagg ctctggtggc cgtcttccag
     2281 ccattgatca agtgtgctgt caaaacggaa cctgggcgtt cggccgatat gtccgaacat
     2341 gccgttcgcc tcgacctcaa ggcgcagcct ttcctttgaa ctgtgcttcc actcgaactt
     2401 catccagagg ttcggagaga cggtccaggc gtcgcgttcc tcaagttcgt atccgtcgta
     2461 gtccgcttca ggcgtgaccg ccagcgcatc gtccatcttc tgcttcgaat cggtcttctg
     2521 ccagcggtag cggacataga cgccccagtc gaacaccggc ttcccggaaa ccagcctgtc
     2581 ggcccgctga agtcttccag ccatcgtcaa gggcttctgg aagatggatg cggtgaagtc
     2641 gaacacgttg tcgctgttcg taaggtcgta cggctgcccg taagcctgga tgtccgtcgc
     2701 cgaggtggcc ccagtcgcgg tccatccgaa cccgagttcc agatacacgt tccagtagcg
     2761 ggcaaggacg ttcacgcgat cgacataggt accgaaatcg gcgtcgatgt cgccgccgtc
     2821 gttgtccatc gtgccaagac cccagtgacg ggccatgcgg ccggccgaga tagacagaag
     2881 gaagtccttc gtgttgtccg ggttgaaggg gctccagatc atgtaggcct gcttgacctt
     2941 caccgattca tcgggaacgg cctgcgacct tgcgaaaatc gaaagcgggg cgtccggcct
     3001 gtcggcgtcg gaatccgggg tcgaacccag tatcaggttg tccaggacgt cgaactgcgc
     3061 cacgatggcg atgtttttca ttacatggat ggtcggtatc cagcggaacc ggatgttcat
     3121 gctgctcaac cagtcctctt gcgaaaggcg atccttgttg atgtagtttt ccgttattgg
     3181 cgaagggtaa gccgaagagt tgtacgcctt ctgcgttcga ttgtcataag ccgtcgtgcc
     3241 aaggtgcggc ctgtagaatc cgtccatccg aagcctgaag tatccgtcgt gttcgatctt
     3301 cgggtaaccg gccggggaaa gcggcctgtt gcggatgcca ccaagagcct cttccctgat
     3361 cttcgcagcc tccgcgcgcg caaccgggtc gtcggccctg gcccgatcgg acgtcctggc
     3421 ggcggccttt tcgtcggtcg gcctgtcttc ggcgtcgttt gccgccccgg tcgagtcgtc
     3481 attcgaatcg ggcgcctttc cggtttcgct ggccggctcg gttgccggtt catcggcggc
     3541 agcttccggg ggcgttgtcg ccggttcatc cgtcgctgct gcctgttctg cggcaaccgg
     3601 ctccgccggg ttctgatcag gttcggcggc agtggtcgcc gatgatgcaa gcatcagcat
     3661 catggcaagc gttgccggca gtcgaacgaa agtcggggta aatcgtttaa tccccgcagg
     3721 aaaaggaatc ttcatgcgat tcatcgcctg gttaacgggc gctttactct aaacgagcag
     3781 ccggggccgg tcaagagttc agggcgtgat tgactggggc cctcgaatgt ggtcaaattc
     3841 agggccggtc aaagatgaag ccggtttgta gcttttccgg attgttccgg aacaggtgca
     3901 ggaggatccg atgaagagta ttgctgtcgc ttcttttgtg acgtcgtcga ttttgttcgc
     3961 gtgttccgcc atggcccagg acgcgcttcc cgagcccgcc gccgagtgcg tgccgaaggc
     4021 cgaggcgccc gtcgcgcctg tcgataaccg accgataatt aacaagaaat ccttgaagat
     4081 gcgcggttac gacgtgatcg agcaggttga actggcacag ttcgggccgg gatggtcggc
     4141 cgtcctgctc aagctggccc tgccggaagc tgaaaacgag gcacttgccg aagtcagtcg
     4201 tctttacatt cttgagggcg atcgcatcga attcgactcc ttcgcgtttg acgccatcga
     4261 agacggcatc gaaccgagcg tcagcaccag aacctttttc cggatgaagt gggaagtgac
     4321 gatgaaggga aagagtccct ccggcctgat ccttaccggt atcgtcccgc gccagcttcc
     4381 cggcgacaag atccgtgtcg ccaccaggac gcttgtactt acgtggaaca aggccagcgg
     4441 tttccgcgaa gtggccgacg tggtttcgcg cgagcccgcc cgcgtcaacg gcggcatcat
     4501 gaccgttaca gtcaactgaa ttcgggttct ccataaatga ctgaaaattc ccaaccggaa
     4561 ggcattctgt ccgggctcaa tccggagcag ttcgccgccg cgacccacga gtcggggccg
     4621 ttgatgattc tggccggtgc cgggtcgggc aagacaagga cgattacgca ccggatcgcg
     4681 tggcttatcg gcaaccgggc cgtccatcca tcatcgatca tggcggtcac cttcacgaac
     4741 aaggccgcac aggagatgcg cgacagggtt cgggcgcttg tcggaagcga cgccacgccc
     4801 gggtggatgg gcactttcca ttcggtctgc ctgcgcctgc tgcgcattca cggggccagg
     4861 cttggattca agcccgattt tgtcgtttat gacgacgacg attccgaaag gcttctgaaa
     4921 ctggtgctgg attcgatggg catcccgcgc gacggagtca ggcagtattc gaacgccatc
     4981 gaccggttga agaacgacgg cgttgtccac ccacccgagg ccgggaccct tcgggagcgg
     5041 gccatcgccg acatcttcaa gcggtaccag gcggaacttt tcaaggccgg ggccatggat
     5101 ttcggggact tgctggtaat ggcggcccgg ctcctgcgcg agaacgacga tgtccgggag
     5161 tcgctgcagg caaggttttc ccacctcatt gtcgatgagt tccaggacac caacctggtt
     5221 cagtacgact tcctgaagtt gctttgtcgt ccggatcccg acatctgcgt cgttggcgac
     5281 gacgaccagt cgatatactc ctggcggggc gcccgcgtca gcaatatcct tgatttcgcg
     5341 actgattttc ccggcgtgac gaaagtcgtg ctgggaagga actacaggtc gtcggccaac
     5401 gtgctgggtg ccgcttccgg cgtcatccgc ttcaacaggg acaggtatcc gaaagatttg
     5461 caggcggtcc gtggaatcgg tgaaaaagtc tatgtcaagc aactgctgaa cgaggcagga
     5521 gaggcggcat tcgtcgtcga tgaacttgcc tcgaggcacc ggcgcggctt cgccctgtcg
     5581 cgaatggcgg tgttttaccg caccaacgcc cagtcccgag tgttcgagga taccctgcgt
     5641 cgaagaaaga tcccgtaccg ggtaatcggg ggaatgcgct tctatcacag ggccgaaatc
     5701 aaggacatca tctcttatct gcgactgctg gtgaatccga tggactcggt ggcgcttgac
     5761 cgtgtggtga acgttccggc acgcggcatc ggcaagacca cgatggacaa ggtcagggcg
     5821 cattccgatt tggaaggcat ccctttgatg caggcgctcg tcgaggtggg cgatcagtcg
     5881 ggcaaggcgg ttcgagcgcg tatttgagct tttgtcgaga tgatctgcga tcttgcatcc
     5941 ttcgc
//
LOCUS       DAOPPA010000170         5910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14733_length_5910_cov_4.157472, whole genome shotgun sequence.
ACCESSION   DAOPPA010000170 DAOPPA010000000
VERSION     DAOPPA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14733_length_5910_cov_4.157472"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKH54_06480"
     CDS             <1..507
                     /locus_tag="PKH54_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC99570.1"
                     /translation="RRPRTRGSLLFTHFGLSGPAVLDISGDVSRMLDEGTPVTLSIDL
                     LPDTPAKEVDEMLQSDPRCIQGRLPSRLAAFLKDGAISDWRPGRPNPIKRFTVKIART
                     RGFDHAMVTRGGIVTGEIDPATMQSRLHPGLFFAGEILDLDGPMGGFNLQIAWATGHL
                     AGTSASAG"
     gene            628..1680
                     /locus_tag="PKH54_06485"
     CDS             628..1680
                     /locus_tag="PKH54_06485"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOC99571.1"
                     /translation="MNIPEIQPDEPVAMPARKPAGPVMRIVATVTVVMGVAGIAWIIH
                     SVGLDSLGQVMSNLSIPKFLVLLGWYYLMFLFDTLAWRQLIRKADRPSVWALWKAAIA
                     GASINQLTPGGNIGEPLKIMVLKNKGDLKEIIASLVAWNFMHLSTTMVCVLVGAIPIF
                     MFLKADFTFVLLYLGGALILGLPAALILATFRYNILSRMASLVARLGFKADRLMNWQQ
                     KVHSIEKNVVAVIRERPKDVAISFVHLLASRFISITMTFGMVWVLNIDIPFVTVIYVQ
                     MLNLAVSTLFSFVPARVGVFEGYNAIIFKALEYTPQAGLAVSIITRVIQVITTVIGVL
                     ILARPMLDARHRDGRR"
     gene            1688..2869
                     /locus_tag="PKH54_06490"
     CDS             1688..2869
                     /locus_tag="PKH54_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC99572.1"
                     /translation="MKYFIIDNVGRWAGRKLLGLLPTTRAVFQARAAGAREVTILSDT
                     PDGRTVPKGCPDDVRIGFAPLDRGEASLTDLAQRLAAESGDQVFVMDANFIFQRDFAP
                     DAATRPENTVFSAGGKQVAAIRVQEGAYKEDCACGRANEIETAGGVASAKKMLYQGLK
                     KPLLVNGLVAAFLQRPMAHVVTVAIVNTPIRPNHVTIFAGLVGVAGAVVTLLAGSRPW
                     MMQLGVLLYFLGSVFDCIDGDLARLKYQFSYIGSWLDTIADDASTAAIMFALAFYQAQ
                     LTGDMNWFYFGAGAAGIFVLTELYIYYHLARYYHSGDVLDFQWAVGVKKRASAESFVD
                     YVILFFKRDFFTLLLLVLGLFQVVHAGLVYMSVLMYLFGGYVLLDVFLSLRRPGWRTR
                     R"
     gene            2896..3600
                     /locus_tag="PKH54_06495"
     CDS             2896..3600
                     /locus_tag="PKH54_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOC99573.1"
                     /translation="MGNRAIILAAGISSRIFELTKGLPKGLMEINGEPVVGRIIRLLR
                     EAGVFDITVVVGYREELFRERFPECNFVTNPEFRSTNTCVSLELALKANETDSVFVIN
                     GDVYFEESILKQMLACGHPTVAAVSRHPLCDEEIKVHVKDGLVTTIGKHLNDEIAYGE
                     AFGIYLMSPKFSVYMKRALNLLNNPKVFYEAAMDHLLAGGHVMNILDVGDAVVQELDF
                     PTDYEALMKTISAKSE"
     gene            3604..4347
                     /locus_tag="PKH54_06500"
     CDS             3604..4347
                     /locus_tag="PKH54_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC99574.1"
                     /translation="MGFSDRYRALLPGLKVRGNTHNLDFWGIVFGRPVAFSILLLIGD
                     IPWLTPNMLTHASNAFLVAGAAAILVGTWPWFMVAVALLNISYAFDCADGQLARYRKN
                     GSLVGSYYDKMSDHFGMTLIYTALAWVAFQRNPQPLYFLLAIAAISGNLIIGYAKWIA
                     AAHGKLVESTGASEDSGLLRAGLIVLRKGIEFRDADIFLWCSLGLILDHPEIGLVLMG
                     ISQFVVAIISSFHRGWLMSRPSPPDSGNH"
     gene            complement(4373..5446)
                     /locus_tag="PKH54_06505"
     CDS             complement(4373..5446)
                     /locus_tag="PKH54_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HOC99575.1"
                     /translation="MVRLFHDAGVLPQLLDGETPLFITMPGETLRLADPGGVVSDSIR
                     RTGAIHLSYAGTALPVQDVQRLYRQVIPERCSIIVAVGGGTVIDIAKIIGVAVSNRLP
                     ALENLLDNPAGFVNTVPTICVPTTCGTGSEATHFAVVYSGGRKYSIAHQSMRPGIAIL
                     DWRFLVALPERIRNATVLDALSQAIESIWAKGSNDDSRQYAVEAVGSILRGLDATTDA
                     DRLKSLQDGSYLSGKAIDISKTTAAHAISYPLTARFGIPHGIAVFLTLPGIAELNYQV
                     GLCDRFKLLFDLFHVDSISAFQARLRDIMAGMGFSARLSDWGVADGDLSAIAAESITP
                     GRSDNNPAVVSPELVLKLLRSIM"
     gene            complement(5427..>5910)
                     /locus_tag="PKH54_06510"
     CDS             complement(5427..>5910)
                     /locus_tag="PKH54_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC99576.1"
                     /translation="QMSHANDFYTVGSMGCASSIALGVSHGDHNRPIFILDGDGAALM
                     QMGTFAALGKHGQDRKITHVLIDNQAHESTGGQPTLSSTVSFQRIAEGCGYQAVLVAN
                     NLQEFEDCLGRIGGNDSLKLLVVRSRKGSRPDLGRPKTSPIENRINFMESIRNGQAVS
                     "
BASE COUNT         1214 a         1749 c         1639 g         1308 t
ORIGIN      
        1 cggcggccgc gcacgcgggg aagcctgctt ttcacgcact tcggcctgtc tggaccggcg
       61 gttctcgata tcagcgggga cgtttcccgg atgctcgacg aaggcactcc agtgaccctt
      121 tcgatcgatc ttcttcccga cactccggcg aaagaagtcg atgagatgct ccagtccgat
      181 ccccgatgca ttcagggacg acttccttcg cggctggcgg ccttcctgaa ggacggggca
      241 atttcggact ggcgtccggg acggccaaac ccgatcaaac gtttcaccgt gaagatagcc
      301 aggacgcgcg gtttcgatca tgcgatggta acgcgcggcg gaatcgtgac gggcgagatc
      361 gacccggcca cgatgcagtc caggcttcac cccggtcttt tcttcgccgg cgaaatcctg
      421 gatcttgacg gcccgatggg cggtttcaac cttcagattg cctgggcgac cgggcatctt
      481 gccggcacat cggcgtccgc cgggtaacct gccgggaacg gatcccggcc gcgccgtgga
      541 ctgcgtaatg cctttattta cgtccccaat cgtggcaaca ttcgcggggt cgaaagactt
      601 atccacttgt ggatcttgtt attggtcatg aacataccgg aaatccagcc agacgaacca
      661 gttgcgatgc cggcccgcaa gccggccgga cccgtcatgc gaatcgtcgc gactgtcacg
      721 gtcgtgatgg gcgttgccgg aatcgcctgg attattcact cggtcggcct cgactcgctt
      781 ggacaggtga tgtccaacct ttcgattcca aagttcctgg tgcttcttgg atggtattac
      841 ctgatgttcc tgttcgacac gcttgcgtgg cggcagctta tccgcaaggc ggatcgaccg
      901 tcggtctggg cgctttggaa ggcggccatc gccggcgcct cgatcaacca gcttactccc
      961 ggtggcaaca tcggcgaacc attgaagata atggttctta aaaacaaggg cgacctgaag
     1021 gaaatcatcg cttcgctcgt cgcctggaac ttcatgcacc tttcgaccac gatggtctgc
     1081 gtccttgtcg gcgccatccc aatcttcatg ttcctgaagg ccgacttcac cttcgtgctg
     1141 ctctatcttg gcggcgcgct gatcctgggc ctgcccgccg cgctgatcct tgcgaccttc
     1201 cgatacaaca tcctcagccg tatggcatcc ctggtcgccc gccttggctt caaggcggat
     1261 cgtctgatga actggcagca gaaggttcac tcgatcgaga agaacgtggt cgcggtcatc
     1321 cgcgaaaggc cgaaggacgt cgcgatctcc ttcgtccatc ttctggcttc gcgcttcatc
     1381 agcatcacga tgaccttcgg catggtctgg gtgctgaaca tcgacattcc gttcgtcacg
     1441 gtgatttatg tccagatgct taaccttgcg gtaagcacac tgttttcctt tgttcccgcc
     1501 agggtcggtg tgttcgaagg atataacgcc atcattttca aggcactcga gtacaccccc
     1561 caggccggtc tggccgtatc cataatcacc agggtcatcc aggtcatcac gaccgttatc
     1621 ggagtactga tacttgcccg cccgatgctg gacgcaaggc accgcgacgg acggaggtaa
     1681 aagacgcatg aaatacttca tcatcgacaa cgtcggccga tgggccggac gcaagctgct
     1741 tgggctgctg ccgacgacca gggccgtctt ccaggccagg gcggccggcg caagggaagt
     1801 caccatcctt tccgacacgc cggatggccg aaccgtcccc aagggctgtc ccgacgatgt
     1861 ccgaatcggt ttcgcgcccc ttgatcgggg tgaagcatcg cttaccgacc ttgcccaaag
     1921 gcttgccgcc gagtccgggg atcaggtgtt cgtgatggac gccaacttca tcttccagcg
     1981 tgacttcgcg cccgacgccg cgacccgtcc cgaaaacacc gtcttttcgg ccggtggaaa
     2041 acaggtcgcc gcaatccgcg tgcaggaagg cgcctacaag gaagactgcg catgcggccg
     2101 tgcaaacgag atcgagacgg cgggcggggt cgcgtccgcc aaaaagatgc tttatcaggg
     2161 actcaaaaag ccgctgcttg tgaacggact tgtcgcggcc ttcctgcagc gcccgatggc
     2221 gcacgtggtc accgtcgcaa tcgtcaacac gccgatcagg ccgaatcacg tcacgatttt
     2281 cgcgggactg gtcggggtcg ccggggcggt cgtgacgctg cttgccggtt caagaccctg
     2341 gatgatgcag cttggcgtgc tgctttactt cctcggatcg gtcttcgact gcatcgatgg
     2401 cgaccttgcc cggctgaaat accagttcag ctacatcgga tcctggctgg acacgatcgc
     2461 cgacgacgca tcgaccgcgg caatcatgtt cgcgcttgcg ttctatcagg cccagcttac
     2521 cggcgacatg aactggttct atttcggcgc cggggccgcc ggaattttcg ttctgaccga
     2581 gctttacatc tattaccacc tggcacgcta ctaccactcg ggcgacgtcc tggatttcca
     2641 gtgggccgtc ggggtcaaga agcgggcaag cgcggaatcc ttcgtcgatt acgtcatcct
     2701 gttcttcaag cgcgacttct tcacccttct acttctcgtg ctcgggctgt tccaggtcgt
     2761 tcatgctgga cttgtttata tgtccgtatt gatgtacctg ttcgggggat acgtcctgct
     2821 tgatgtcttc ctgagcctgc gcaggccggg atggcgaacc aggcggtaaa gcccaccaac
     2881 tgttggagaa tcatcatggg aaacagagca atcatccttg cggccggcat cagttcgcga
     2941 atcttcgaac tgacgaaggg ccttccgaag ggcctgatgg agattaacgg cgaaccggtg
     3001 gtcggcagga ttatccggct gctgcgggaa gccggcgtct tcgacatcac cgtggtcgtg
     3061 ggataccgcg aggagctttt ccgcgaacgc tttcccgaat gcaatttcgt gacgaacccg
     3121 gaattccgtt cgaccaacac gtgcgtttcg cttgagctgg cgttgaaggc caatgaaacc
     3181 gacagcgtct tcgtgatcaa cggcgacgtg tatttcgagg aaagcatcct gaagcagatg
     3241 cttgcctgcg gccacccgac cgtcgccgcc gtcagccgcc atccgctgtg cgacgaggaa
     3301 ataaaggttc acgtcaagga cggcctggtc accacgatcg gcaagcacct gaacgatgaa
     3361 atcgcttacg gcgaggcttt cgggatctac ctgatgtcgc cgaagttctc ggtctatatg
     3421 aagcgtgcct tgaacctgct taacaacccg aaggtctttt acgaggccgc catggatcac
     3481 ctgctggccg gcggccacgt gatgaacatc cttgatgtcg gggacgcggt cgtccaggaa
     3541 ctggatttcc cgaccgacta cgaagccctg atgaagacca tttccgcaaa gtcggagtaa
     3601 aagatgggat ttagcgatcg ataccgagct ctcttacccg ggcttaaggt caggggcaat
     3661 acccacaacc ttgacttctg gggaatcgtg tttggccgtc ctgtcgcctt ttcgatactg
     3721 ttgctgattg gcgacatccc atggctgacc cccaacatgc tgactcatgc gtccaacgca
     3781 tttctggtgg caggcgcagc ggccatactg gtgggaacat ggccctggtt catggtagcc
     3841 gttgcgctgc tgaacatttc ctacgccttt gactgcgcgg acggtcaatt ggcaaggtac
     3901 cgcaagaacg gatcgcttgt cgggtcatac tatgacaaaa tgtccgacca tttcgggatg
     3961 acactgatat acacggcgct ggcttgggtc gctttccaaa ggaatcctca gcccctctat
     4021 tttcttctgg caatcgccgc catcagtgga aatctgatca taggctatgc caaatggatc
     4081 gcggcagccc acggcaaact ggtcgaatct accggtgcga gtgaggattc cgggctcctg
     4141 cgtgccgggt taatcgtcct gcgaaagggg attgaattcc gtgacgcgga tatcttcctc
     4201 tggtgttccc tgggccttat cctcgatcat ccagaaattg gacttgttct gatgggcata
     4261 tcccagttcg tggtcgcaat aatctcatct ttccaccgtg gctggctgat gtcgcgtcct
     4321 tcaccgcctg attccgggaa ccactgaaaa aacggacgcg cgcgttgatt gatcacatga
     4381 ttgaccgcag aagtttaagc accagttccg gactcacgac agcagggttg ttgtccgacc
     4441 tgcctggagt aatagattct gcggcgatag cggacaaatc cccgtctgcc acgccccaat
     4501 ccgacaatcg tgcgctgaat cccataccgg ccatgatatc ccgcagtctg gcctgaaagg
     4561 cactgatgga gtcaacgtga aaaaggtcga agagcaactt gaacctgtca cataaaccaa
     4621 cttgatagtt caattccgct atgccgggaa gcgtcagaaa cactgcgata ccgtggggta
     4681 tcccaaacct tgcagtcagc ggatacgaaa tggcatgggc cgcagttgtc ttggaaatat
     4741 cgattgcctt gcccgaaaga tagctgccgt cctgcagact cttcaaccta tccgcatcag
     4801 tcgttgcatc caggccccga agaatgcttc ctacagcttc gacggcatac tgcctggaat
     4861 catcgttcga acctttggcc cagatcgatt cgatggcctg tgacaaggca tcaagcaccg
     4921 tggcattccg gatcctctcg ggaagggcca ccagaaaacg ccagtcaagt atcgcgattc
     4981 ccggcctcat ggattgatgg gcgatcgagt acttcctgcc gccgctatac accaccgcga
     5041 aatgagtcgc ctcactgcca gtcccgcaag tcgtcggtac gcagatcgtt ggcaccgtgt
     5101 ttacgaaccc agccggattg tccagtaagt tctcgagcgc cgggagtcta ttggaaaccg
     5161 ccacgccgat aatcttggca atgtcgatta ccgtcccacc accgaccgcg acgattattg
     5221 aacagcgctc gggaataacc tgtcgataca accgctggac atcctgaacc ggaagcgccg
     5281 tcccggcgta tgaaaggtgg atggcgccag tccgccggat cgaatctgaa actacaccgc
     5341 ccggatctgc cagacgaagg gtctcgccag gcatggtaat gaacaatggt gtctcgccgt
     5401 caagcagttg cggcagcaca cccgcgtcat gaaacagcct gaccattcct gattgattcc
     5461 atgaaattaa ttctattctc gatcggtgag gttttcggcc tcccaagatc gggcctggat
     5521 ccctttctgg atcgtacgac cagcaactta agactgtcgt tgcccccgat cctgccgagg
     5581 cagtcttcaa attcctgaag gttgttggcg acaagaacag cctgataccc gcaaccctcg
     5641 gctattctct ggaaagatac cgttgaagaa agcgtcggct gaccgccagt cgattcgtgt
     5701 gcctgattgt caatcaggac atgagtaatt tttctgtcct gtccgtgctt cccaagcgcc
     5761 gcgaaggttc ccatctgcat gagagccgcg ccgtcgccat caagaatgaa aattgggcgg
     5821 ttatggtcac cgtgggacac gccaagggct atcgaggacg cacagcccat cgacccaact
     5881 gtatagaaat cattggcgtg gctcatttgc
//
LOCUS       DAOPPA010000171         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14743_length_5908_cov_5.841107, whole genome shotgun sequence.
ACCESSION   DAOPPA010000171 DAOPPA010000000
VERSION     DAOPPA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14743_length_5908_cov_5.841107"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PKH54_06515"
     CDS             <1..1068
                     /locus_tag="PKH54_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99577.1"
                     /translation="IAGVRTSARDCVDELVKAVDFKKFDSTLNDVLKESNSLLSTPVL
                     ILYGLERSRGARDFSYGNYDFKEHELFKGAPRPIDKNCGPEKQHIVPYSLLTQAYGVE
                     SGGRISSHPINNIGNLTYLSHDMNGLDGLGSTPLDFTGEEQGNLDAHCLGAESLGLYR
                     KIQPLLNADMAIGANRECLKDLFDKWTEARRQHIKEGFVQWLAELKSGWVDSDARKGD
                     GMARIEPETPKLMGAAGSADLEYLIRKLDLDNRIEDRLIQLFGDSGWYPTGSDDSCLT
                     LCWKHRNRQWIRNVRLDKQGLSAEINRIPQAVQFEPGLGVSDALGEFIGRILNCQQEA
                     RDHARRRDEEKKASKARIPPR"
     gene            complement(1181..2167)
                     /locus_tag="PKH54_06520"
     CDS             complement(1181..2167)
                     /locus_tag="PKH54_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC99578.1"
                     /translation="MSKFKPQYRRLLFIDQRLRDACGAESLPNCNTLAAEWETSPKTI
                     QRDIEFLKWEMGAPIVYDRARHGYRYSEPDFVLPALRMSEQDLFAFCLAEKVLQQYEN
                     TPLHSHLASIFSKIEQYLPENVTVVPALLDGRVSFFAEATTTIDPAIWEAVFQALRLG
                     RTLNITYQIPGRDKPYDTRVDPYHVVGYRGEWYVVGRCHLKMALRVFGISRMHAAAIS
                     SDRFTLPPDFDFEQQWRNHFGIMIGGEETTVRVRFRQDQAPYVRERDWHPAQQFTDEP
                     DGSLTMTFKTRHLLEVTRWLLSWGSAATVLEPPELVAAVQSELTAAATNYGG"
     gene            2350..2667
                     /locus_tag="PKH54_06525"
     CDS             2350..2667
                     /locus_tag="PKH54_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HOC99579.1"
                     /translation="MDINVTVNLVARDLRFMRAAHDVLKRYRFHRLEPASPGDPIRFR
                     VEGGRKEYQITINPRWAAKPACTCPDAAQQGGRLNGGYCKHIIAVLIREEALQYQLLE
                     ILL"
     gene            2687..3775
                     /locus_tag="PKH54_06530"
     CDS             2687..3775
                     /locus_tag="PKH54_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC99580.1"
                     /translation="MADARGDASVVRRIEGYSYAELKELVEHTLRAGVSVLVRGHPGV
                     GKSTLAGELAAAMGLPLVDIRLAQRDPAELAGVYFPDRDRRELALFPPDWVRQACAEP
                     VMVFLDEINAAVTRLHQAAAYQIVLEKRVGPFVFHPGTVVMAAGNLEEDNAIVSSLSS
                     ALCNRFAHYILRVDSRAWLDWGSGAGIDDALLAYIGRYGEEVLYENTGDYAFATPRSW
                     EMASRLMAGAPPEMHKRLIASCVGLAAAEKFGNYQRIYQQVNARKIIEKGQKMNFTRG
                     RSAEPSFIYAAVFAVAAFLNSTPEIADEHLPNIVAFARSPGLDPEYVFLFLRHLKRHP
                     NLLERLKVLPEYQALAGELVALHMGLYQ"
     gene            3772..5091
                     /locus_tag="PKH54_06535"
     CDS             3772..5091
                     /locus_tag="PKH54_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOC99581.1"
                     /translation="MTGKPDSKAGGKAAVMADVQTDTPSDVIAAEARRVSFLRMQMLQ
                     MHPFWGYLLLQMKLVAAPELEAFAATDCIRHIWYNPLLTCHLSMGQLGFVLAHEVGHQ
                     IFASEGRRRGRNLSLWNCATDYAINRIVANIGNPARPNSPLYEPPQGEIPGLGNVRIL
                     LDDRWKGMIAEAIYEYLAAEELPEPVSVTIHLTGGEGDDGGGDFCVPNLTDHCGGIDI
                     HLPENLSARQRGELIERLSGALETWERQDGVGDMPGNVVRNILARGRPLVPWQRVFRQ
                     FAGQAVSRDDYSLSRPNRRYMQEDLVVPGLYSEKAGHVVVALDTSGSMSEAALRAVAV
                     ELNALSQAAVELTLIVADASVHEVVGPDRIENFLKAGRFRGGGGTDHRPVFNWIRNAR
                     LRPDLFVGMTDLHSRFPERRPPWPVLWVVPAADHGVAPWGRVIEVRE"
     gene            5088..5762
                     /locus_tag="PKH54_06540"
     CDS             5088..5762
                     /locus_tag="PKH54_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99582.1"
                     /translation="MNLHKHHPSINPHARKWAVRPIPHPAGPRKGDLYFVQSDMRYMG
                     AVYTIRKINGRSFYYYRFETCKNPGVVFRRLLVEWEQWIDELFDQGFVFCNGTALLPP
                     PRLTPAVPVDTGLDQNRKQAELAMADARRILAEHPIGDPVRVGDESRFTVDCGDGLDV
                     TVTVPDDVSLPVACSCRKRVESVDGSVPSDSSGGHADPVCPCTHAFAVLIGRGEMTHR
                     LLSFLL"
BASE COUNT         1155 a         1632 c         1935 g         1186 t
ORIGIN      
        1 atcgcgggcg tcaggacgtc cgcccgggac tgcgtcgacg aactggtcaa ggctgttgat
       61 ttcaagaaat tcgattccac gctaaatgat gttttgaagg aatccaattc gttgctgagc
      121 acccccgtcc tgattcttta cggccttgag cgctcaaggg gtgcgcgcga tttcagctac
      181 gggaactatg atttcaagga gcatgaactg tttaaaggcg cgcctcgtcc aatcgacaaa
      241 aactgcggtc ctgaaaagca gcatatcgtt ccgtattcat tgctcacgca ggcctacgga
      301 gtggagtcgg gcggcaggat cagctcgcat ccaatcaaca acattggaaa cctgacctac
      361 ctttcgcacg acatgaatgg ccttgacgga ttgggctcga cgccgcttga ttttaccggt
      421 gaggagcagg gcaacctgga cgcacactgt cttggggccg agtcgctggg tctctaccgg
      481 aagattcagc cgcttctaaa tgcggatatg gcgataggcg ccaacaggga atgtctcaag
      541 gaccttttcg ataaatggac tgaggctcgg cgccagcaca tcaaggaggg attcgtgcag
      601 tggcttgccg agctgaagtc gggatgggtt gattcggacg cacgcaaggg cgacggcatg
      661 gcccggatcg agcccgagac accaaagctg atgggggctg cgggatcagc cgacctggaa
      721 tacctgatcc ggaagctcga cctggacaac cggatcgagg acagattgat ccagttgttt
      781 ggggactccg gttggtatcc tactggcagt gacgattcct gcctcacctt gtgctggaaa
      841 cacagaaaca ggcagtggat aagaaacgtc aggctggaca agcagggctt gtcggctgaa
      901 atcaaccgaa tcccgcaagc cgttcagttc gagcctggcc tgggtgtgtc tgacgcactt
      961 ggtgaattca tcgggcggat tttgaattgt cagcaggagg cccgggatca cgcccgccgc
     1021 agagacgagg agaagaaagc cagcaaagcc agaatcccac cgcggtaaat catttgcccc
     1081 ctagaagaca tcgaacagcg cctgcccaag tactttgggc ggctgaaatg cctgaaacgt
     1141 caatcttctg cggattgtag cagccgaaat ggacgtgaaa tcaccccccg tagttcgtgg
     1201 cggcggcggt tagttcggac tggacggcgg cgacaagttc gggcggttca aggacggtgg
     1261 cggcggagcc ccaggagaga agccagcggg tgacttccag caggtggcgg gtcttgaagg
     1321 tcattgtcag ggagccgtcc ggttcgtcgg tgaattgctg ggcggggtgc cagtcgcgtt
     1381 cgcggacgta gggggcctgg tcctggcgga aacgcacgcg aaccgtggtt tcttcaccac
     1441 cgatcatgat gccgaagtgg ttgcgccact gctgctcgaa gtcgaagtcg ggcggaaggg
     1501 tgaagcggtc ggaagagatg gctgccgcgt gcatcctgga aatgccgaag acgcgaaggg
     1561 ccatcttcag gtggcagcgg ccgaccacgt accattcacc ccggtacccg acgacgtgat
     1621 aggggtccac ccgggtgtcg taaggtttgt cacgtccggg aatctggtag gtgatgttca
     1681 gggtgcgccc cagtcgcagg gcctggaaaa cggcctccca gatggcgggg tcgatcgtgg
     1741 tggtcgcctc ggcgaaaaac gagacccgcc cgtccagcag cgcaggaacg accgtcacat
     1801 tctcgggaag gtactgttcg attttggaaa agatggatgc caggtgcgaa tgcagcgggg
     1861 tgttttcgta ctgctgcaac accttttccg ccagacagaa agcgaacaga tcctgttcgg
     1921 acatgcgtaa cgcgggcagc acgaaatccg gttccgaata gcggtaaccg tgcctggcgc
     1981 ggtcgtaaac gatcggagcg cccatttccc acttcaggaa ctcgatgtcg cgctggatgg
     2041 tcttggggct ggtctcccat tccgccgcca gagtgttgca gttcggcagt gactccgcgc
     2101 cacacgcgtc gcgaagcctt tgatcgatga aaaggagcct gcggtactgc ggtttgaact
     2161 tcgacatgtg cgcctcacgc gaaacggctt ccatagtaac ggacaaatcg tgaccgatac
     2221 cccctttttt ataccagttc gggtctgctt gtcgctgatt tcggacgttt gcgaaatatt
     2281 ccggactatc ggacatcatc tgaccgatgc ccctgattat gatgggcgca tcgatgaggt
     2341 gactccgaaa tggacatcaa cgtcacggtc aatctggtgg caagggacct gcgtttcatg
     2401 cgggccgccc acgacgtctt gaagcggtat cggtttcatc ggttggaacc ggcttcaccc
     2461 ggcgacccca ttcgattccg ggtcgagggc ggccgcaagg agtaccagat aacaatcaat
     2521 ccgcgctggg cggcgaaacc ggcctgtaca tgccctgacg ccgcgcagca ggggggccgg
     2581 ctgaacggcg gttactgcaa acacatcatc gcggtgctga tccgcgaaga ggcactccag
     2641 tatcagttgc tggaaatcct gctgtaaggc gggaaggggg tatgagatgg cggatgccag
     2701 aggcgacgcg tcggtcgtgc ggcgcattga agggtacagt tacgcggaac tcaaggaact
     2761 ggtggaacac actctcaggg ccggcgtgtc ggttctggtg agggggcatc ccggtgtcgg
     2821 caagtccacc cttgcaggtg agctggcggc cgcgatggga ttgccgctgg tcgacatccg
     2881 gctggcgcag cgcgacccgg ccgaactggc cggggtctat tttccggacc gcgatcgccg
     2941 ggaactggcc ctgttcccgc ccgactgggt gcggcaggcc tgcgctgaac cggtgatggt
     3001 cttcctggac gaaatcaacg cggccgtgac caggctgcat caggccgcgg cctaccagat
     3061 cgttttggaa aagcgggtgg gccccttcgt tttccacccc ggcaccgtgg tcatggccgc
     3121 cggcaacctg gaagaggaca acgccatcgt cagttcgttg tcgtccgccc tgtgcaaccg
     3181 gtttgcgcac tacatcctgc gcgtcgattc gcgggcgtgg ctggactggg gcagcggcgc
     3241 cggtatcgat gacgcgctgc ttgcatatat aggtcgctac ggtgaagagg tcctttacga
     3301 aaacaccggc gattacgcct tcgccacacc gcgaagctgg gagatggcga gccgcctgat
     3361 ggccggcgct ccgcccgaga tgcacaagcg gctgatcgcg tcgtgcgtcg gactcgcggc
     3421 cgctgaaaaa ttcggaaact accagcgaat ctaccagcag gtgaatgccc ggaagatcat
     3481 cgaaaagggt cagaagatga atttcacccg gggccgttcc gccgagcctt cgttcatcta
     3541 cgccgcagtg ttcgcggtgg ccgcgttcct gaacagcacg ccggaaatcg ccgacgaaca
     3601 tctgccgaat atcgtggcgt ttgcacggtc gccagggctc gatccggaat acgtcttcct
     3661 gtttctgcgg cacctgaaac ggcatccgaa cctgctggaa aggctcaagg tgctgccgga
     3721 atatcaagcg cttgccggtg aactcgtggc gttgcacatg gggttgtatc aatgacggga
     3781 aagccggaca gcaaggccgg ggggaaagcc gctgtgatgg ccgatgtgca gacggacacc
     3841 ccgtccgatg tgattgcggc cgaggcgcgg cgggtttcgt tcctgcggat gcagatgctt
     3901 cagatgcatc cgttctgggg ctaccttttg ctgcagatga aactggtcgc ggcgccggaa
     3961 ctggaagcgt tcgccgccac cgactgcatc cggcatatct ggtacaaccc cctgctgacc
     4021 tgtcatctgt cgatgggtca actcgggttc gttctggccc acgaggttgg gcaccagatc
     4081 ttcgccagcg agggtcgtcg gcgtggcagg aacctttctt tgtggaattg cgccaccgac
     4141 tacgccatca atcgaatcgt cgccaatatc gggaacccgg cgcggcccaa ttcgcctctt
     4201 tacgagccgc cccagggtga aatcccgggt ctgggcaatg tcaggatcct gctggacgac
     4261 cgctggaaag gaatgattgc cgaggcgatc tacgagtacc tggcggccga ggaactgcct
     4321 gaaccggtgt cggttacgat tcatcttaca ggtggcgaag gtgatgatgg cggcggcgat
     4381 ttctgcgtgc cgaacctgac cgaccactgc ggtggaatcg atatccatct gccggaaaac
     4441 ctgtcggcgc ggcagcgtgg ggaactgatc gagcgcctgt ccggggcgct ggaaacctgg
     4501 gaacgtcagg atggtgtggg agacatgccg ggcaacgtgg tccggaacat tctggcgcgt
     4561 ggcagaccgc tggtgccctg gcagcgcgtc ttccgtcagt tcgcgggaca ggcggtctcg
     4621 cgcgatgact acagcctatc ccgcccgaac cggcgttaca tgcaggaaga cctggtggtg
     4681 ccggggctct attccgagaa ggctggccat gtggtggtgg cgctggatac ctcgggcagc
     4741 atgtccgagg ctgccctgcg ggcggtggct gtcgagttga acgcgctttc gcaggccgcg
     4801 gtcgaactga cccttatcgt ggcggatgcg tcggtccacg aggtcgtcgg cccggaccgc
     4861 atcgagaatt tcttgaaggc cgggcgattc cgcgggggcg gcggcaccga ccatcgtcct
     4921 gtttttaact ggattcgcaa tgcacggtta cggcccgatc tgttcgtggg catgaccgat
     4981 ctgcatagcc gatttccgga aaggcggccg ccgtggccgg tgctctgggt cgtgcctgcc
     5041 gcggatcacg gcgtcgcgcc gtgggggcgg gtcatcgagg tcagggaatg aaccttcaca
     5101 aacaccatcc gagcatcaac ccgcacgcca ggaagtgggc ggtgcgtccg attccacatc
     5161 cggcgggtcc caggaagggc gacctttatt ttgtccagtc cgatatgagg tacatgggcg
     5221 cggtctacac catccggaag atcaacgggc gcagcttcta ctattaccgg tttgaaacct
     5281 gcaagaaccc gggggttgtc ttccggcggc tcctggtcga gtgggaacag tggatcgacg
     5341 aactgttcga tcaaggcttt gtgttctgta acggcacagc attgcttccg ccgccacgtc
     5401 tgactccggc cgttcctgtc gacaccggcc ttgaccagaa tcgaaagcag gcggaactgg
     5461 ccatggccga tgcgcgccgg atcctggccg aacatccgat cggtgatccg gttcgggtgg
     5521 gtgacgaaag tcgtttcacg gtcgactgcg gagacggctt ggacgtgacg gtgacggttc
     5581 ccgacgatgt gagtctgcca gtcgcatgtt catgccggaa gagggtggaa tccgtggacg
     5641 gttcggttcc ttcggattca tcaggcggcc acgccgatcc ggtctgtcct tgcacccacg
     5701 cgtttgcggt tttgatcggt cgaggtgaaa tgactcatcg cttgttaagt tttttgttgt
     5761 aatgtcttga tgttccgggg gacggtgtcc agggaacaga gggggtgcag cgtggtttgg
     5821 tttaaagagt cgatggcgga gagggccagg gggtgtctgc tggggcagtt cgcgggggat
     5881 tcgctgggca gtctggtcga gttccaga
//
LOCUS       DAOPPA010000172         5899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14782_length_5899_cov_3.948665, whole genome shotgun sequence.
ACCESSION   DAOPPA010000172 DAOPPA010000000
VERSION     DAOPPA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14782_length_5899_cov_3.948665"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..334
                     /locus_tag="PKH54_06545"
     CDS             17..334
                     /locus_tag="PKH54_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOC99583.1"
                     /translation="MGKKLFLGGLAWATTEESLRAACAEFGEVEEVRVITDRDTGRSR
                     GFGFVTYVNDEDAERAKNEMDGMLVDGRAIKVDFPRERAERSDRPDRGGRSFNGGGGF
                     RRF"
     gene            368..862
                     /locus_tag="PKH54_06550"
     CDS             368..862
                     /locus_tag="PKH54_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC99584.1"
                     /translation="MLPDDRLAQAGSLTVGVISDTHGQLHPAVAGLFRNCDVIIHAGD
                     ICGHGVLEGLRAIAPLCAVAGNCDSPFINPGLQKFELVTLARRLIAVIHDVHALDIDP
                     VATGVSAIIHGHSHQAGILRKGSVLYLNPGSAGPRRFRFRRSVAILKISPSEMTAELI
                     EFDE"
     gene            907..2790
                     /locus_tag="PKH54_06555"
     CDS             907..2790
                     /locus_tag="PKH54_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99585.1"
                     /translation="MKKCMLMLVVVAFVACGSDPAYQADLISGDVVEPVDSVQPDVGG
                     DEGTLRDEGNPDVGVDVPVVPDTTPDTVQPPHDVVEETLVPDAVVDAPPPVCEFYADF
                     DYKCSEFRPETCPGGMCVAEYCIGPDLDPERWDSCGDGECTECEQPCHSDCGPWDKPT
                     GQKIYNDTEKTMTVYVHGFELYTEGQLETMTYGVDTGCKMAENLRWFGPDRPCSDQGG
                     ENSPRQYTSLEYYGQVPAPWLSDADVAEISAHPEEGVEALFRYSLITAKFIRHKMNAT
                     GAERVNLLCHSMGCLIIMNMIEHNMEELAADNVFVRWVTVSGALGGAREANFYDNPEL
                     QTLAAAVGLSSADFVLLQPEVIKDKALVWDHRLHECNNPLFKEMLIHHICTTDPRNVS
                     FFNQPVLNWLIPNVPNDGVLYTPDMYFHKMAPEVQRHTPRGYSLSSSRTFLYNEHNAA
                     KSSRAAAVLAAAGIHGKRRVVLSIDRIELKNDLEKDNAFDFSEGGFSPAEIVPEVEVR
                     FNPYILDNYQEDLAVHQETWADRVAGFFTQEEDTVKTPDYTLFEGPVLDEMTEVWMSL
                     RLLEADAYAAQGINEELLGIGQDTKVLATWSGQVPLVTGHTFEFANDNVAVRVKVRVI
                     DLY"
     gene            2914..4167
                     /locus_tag="PKH54_06560"
     CDS             2914..4167
                     /locus_tag="PKH54_06560"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOC99586.1"
                     /translation="MATRTIGDIDVAGKTVLMRVDFNVPLKNGVITDDNRIVQALPSI
                     RKALDQGAKLVLMTHFGRPAGIGFEPAYSVKPIAARLSELIGREVLVGPESVVGPETA
                     EMVGRLKAGDVLLLENVRFCAGETMPDNAKANPDGKLTPEQQQILDDFVLALGSLGDV
                     YVNDAFGTCHRKHASMFGAAKVIQKKGGAAVAGYLVEKEIRYLHEAVDNPKRPFVAVM
                     GGAKVSDKIKLISTLLGKVDRLLIGGAMAYTLLKARGVEVGRSLVEEDQVNEMKGLLE
                     KAGDKILLPVDHVATDDFAAGSPKVVTGVDIPAELLGMDIGPATIRLYGDELRNAGTI
                     VWNGPMGVFEKPDYAAGTRAVAEAAADATARGAISVIGGGDSAAAVAQMGLDDRMTHI
                     STGGGASLKYLEGKAMPPIEVLDRD"
     gene            4223..4858
                     /gene="yihA"
                     /locus_tag="PKH54_06565"
     CDS             4223..4858
                     /gene="yihA"
                     /locus_tag="PKH54_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOC99587.1"
                     /translation="MKIKTRFILSAVSPEAFPASPRPEVAFVGRSNVGKSSLINALVG
                     TKMARVSSRPGLTQAINFFEVDVAAVPGLSVSRPGTFFPMMLADLPGYGYAQVSRKES
                     RGWASFIDPYLSARDQLVLVVVLVDSTIPIQPGDMEMVQWLRTMNRPFLIAATKLDKI
                     GPSRRLAETNRLAAGFEARVLGVSATSGDGLEELWKIVLANCKKTQSAKSP"
     gene            complement(4850..>5899)
                     /locus_tag="PKH54_06570"
     CDS             complement(4850..>5899)
                     /locus_tag="PKH54_06570"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99588.1"
                     /translation="DERRNLLELAFTHWKDMSMGMKSMLAMTLHRAERKDDARLVWAS
                     VMDGARTEPDRGMFWAQEERSWLWYNDSIENHARAIRTMMELDPEDKSIDDLVLWLLI
                     NKKMNHWKSTRATAEVIYALARHMSARGEIGGRQVISVKAGQLERDFTFDPEASADTT
                     AQMVIDGPDMVEESMSRVSVSTKTPGWALASATWHFSTDTLPDKGDGDMLNIERRYFL
                     KKKTASGVMLEPLADGTVVVPGDEVQVQVSIKSGEPLEYVHLRDPRPAGFEPATQNSG
                     YRWDLGIAWYEEVRDSGQNFFFDRLPRGEYTFRYGIRAALAGTYKAGPATIQPMYAPE
                     FAAYSAGRTITIGSR"
BASE COUNT         1155 a         1716 c         1811 g         1217 t
ORIGIN      
        1 ttcaggagtt tccttaatgg gaaagaagct gtttcttggt ggcctggcgt gggctacgac
       61 ggaagagtcg cttcgtgcgg cttgtgcgga atttggcgaa gtggaagaag tccgcgtgat
      121 caccgaccgt gacaccggtc gctcccgtgg tttcggtttc gtgacttacg tcaacgacga
      181 agacgcggaa cgcgcgaaga acgaaatgga cggcatgctg gttgacggca gggccatcaa
      241 ggtcgatttc ccgcgtgaaa gggccgaaag gtcggatcgc ccggatcgtg gcggcaggtc
      301 gttcaacggt ggcggcggtt tccgtcgctt ctgatcgcct tgctccaggt tcggataacg
      361 gggtcggatg ctgcctgacg acaggctcgc acaggctggc tcgttgactg tcggtgtaat
      421 atccgacact cacggtcaat tacatcccgc cgttgccgga ctgttccgca actgcgacgt
      481 tatcatccac gcgggtgaca tttgcggtca tggcgtgctt gaggggttgc gagcaatcgc
      541 acccctctgc gccgtggccg gaaactgtga ctcaccgttc atcaatcccg gtcttcaaaa
      601 gtttgaactg gtaacgctgg cgcgtcgcct tatcgcggtg atccacgacg tccacgcgtt
      661 ggatatcgat ccggtggcga ctggcgtctc tgccataatt catggccatt cgcaccaggc
      721 cgggattctg cgcaagggct cggttctgta cctgaatccc ggaagcgccg gcccgcgtcg
      781 ttttcgtttc cggcgttccg tggcgattct gaagatttcc ccttcggaaa tgaccgccga
      841 attgatagaa ttcgacgagt aatccggccg atccggtttt tgttttcaat ctgggcaggt
      901 gtcttcatga agaaatgcat gctaatgctt gttgtcgtcg cgtttgtcgc gtgcggttcc
      961 gacccggcct atcaggcgga tctgatttcg ggcgacgtgg tcgaaccggt cgattcggtt
     1021 cagccggacg tgggtggcga tgaggggacc cttcgcgacg agggcaatcc cgacgtcggc
     1081 gtcgatgtgc ctgtggttcc cgatacgacc cccgacacgg ttcagcctcc acacgacgtc
     1141 gtcgaggaaa ccctggtgcc ggacgccgtg gtcgatgcgc cgccaccggt gtgcgaattc
     1201 tatgcggact tcgactacaa gtgttccgag ttccgtcccg aaacctgtcc gggtggaatg
     1261 tgcgtggcgg aatactgcat cgggccggat cttgaccccg aacggtggga ttcctgcggc
     1321 gacggtgaat gcacagagtg cgagcagccc tgtcacagcg actgcggacc atgggacaag
     1381 ccgacgggac agaaaatcta caacgacacc gaaaagacga tgaccgtcta tgtgcacggg
     1441 ttcgaactct ataccgaagg ccagcttgaa acgatgacct atggcgtgga taccggatgc
     1501 aagatggcgg agaatcttcg ctggttcggc ccggatcgtc cttgttccga ccaaggcggc
     1561 gaaaattcgc caaggcagta caccagcctt gaatactatg ggcaagttcc ggcgccgtgg
     1621 ctttccgacg ccgatgtggc cgagatttcg gcccatcccg aagagggtgt cgaggccctg
     1681 ttcaggtatt cgctgataac ggccaagttc atccgtcaca agatgaacgc gacaggtgcc
     1741 gagcgggtga acctgctgtg tcattcgatg ggctgcctga tcatcatgaa catgatcgag
     1801 cacaacatgg aggaacttgc ggcggacaac gtcttcgttc gctgggtaac cgtttccggc
     1861 gcgctcggcg gtgcccgcga ggcaaacttc tacgacaatc cagaactgca gacgctggcc
     1921 gcggccgtcg ggctttcaag cgccgacttc gtcctgctgc agcccgaggt catcaaggac
     1981 aaggccctgg tctgggatca cagactgcat gaatgcaaca atccgctttt caaggaaatg
     2041 ctgatccatc acatctgcac gacggatccg cgcaacgtca gcttcttcaa tcagcccgtc
     2101 ctgaactggc ttatcccgaa cgtgccgaac gacggcgtcc tttatactcc ggacatgtac
     2161 ttccacaaga tggcgccgga agtccagcgc cacaccccgc gggggtactc gctttcgtcg
     2221 tccagaacgt tcctttacaa cgagcacaat gccgcaaaat ccagccgcgc cgccgctgtt
     2281 cttgccgccg ccggcatcca cggcaagcgt cgggtggtac tgtcaatcga caggattgaa
     2341 ctgaagaacg acctggaaaa ggacaacgcc ttcgacttct ccgaaggcgg tttctcgccg
     2401 gccgagatcg ttcccgaggt cgaagtcagg ttcaatccgt acatccttga caactaccag
     2461 gaagaccttg ccgtccacca ggagacatgg gccgatcgcg tggccgggtt cttcacccag
     2521 gaagaggaca ccgtcaagac gcctgactac acgttgttcg aaggccccgt ccttgacgaa
     2581 atgaccgagg tctggatgtc gctgcgcctg ctcgaggccg acgcctacgc cgcccagggc
     2641 atcaacgagg aacttcttgg aatcggccag gacaccaagg ttcttgcaac atggtctggc
     2701 caggttccgc ttgtcaccgg acacaccttt gaatttgcca acgacaacgt cgccgtcagg
     2761 gtcaaggttc gcgtgataga cctctactag atggttgtga aaaaataaac agagtggcac
     2821 aataccgttt cgacggcatc ccgtgccgtt gtaccgatgg cgccggacct tttgcggccg
     2881 gcagttgcac aggaagtttc caggaggctg aagatggcga ccaggacgat cggggatatt
     2941 gatgtggctg gcaagacggt gctcatgcgc gttgacttca acgttccgct gaagaatggc
     3001 gtgatcacgg acgacaaccg tatcgtccag gcgctgccaa gcatccgcaa ggctcttgat
     3061 cagggtgcga agctggttct gatgacgcac ttcgggcgtc cggccggcat cgggttcgag
     3121 ccggcctatt ctgtcaagcc gatcgccgcc aggctgtcgg agctgatcgg tcgcgaagtc
     3181 cttgtcggtc cggagtccgt ggtcggcccc gaaacggccg aaatggtcgg gcgcctgaag
     3241 gccggggacg tgctgctgct tgaaaacgtc cgcttctgtg ccggggaaac gatgccggac
     3301 aacgccaagg cgaatccgga cggaaagctg acccccgaac agcagcagat tcttgacgac
     3361 ttcgtccttg cgctcggttc gcttggcgat gtgtacgtca atgatgcctt tggaacgtgc
     3421 caccgcaagc acgcgtcgat gttcggcgcg gcgaaggtga tccagaagaa gggcggggcg
     3481 gccgtggccg ggtatctggt cgaaaaggaa atccggtatc tgcacgaagc ggtcgataac
     3541 ccgaagcggc cgtttgtggc ggtcatgggc ggggcgaagg tatcggacaa gatcaagctt
     3601 atcagcacgc tgcttggcaa ggttgatcgc cttcttatcg gcggtgccat ggcgtacacg
     3661 ctgctgaagg ccaggggcgt tgaagtcggg cgcagcctgg ttgaagagga tcaggtcaac
     3721 gagatgaagg gccttcttga aaaggccggg gacaagatcc tgctgccggt cgatcatgtg
     3781 gcgaccgatg atttcgcggc cggctcgccg aaggtcgtca ccggggtcga tattcccgcc
     3841 gaactgctgg gaatggatat cgggccggcg acaatccgtc tttacgggga tgaacttcgc
     3901 aacgctggca ccatcgtctg gaacggtccg atgggcgtct tcgagaagcc ggactatgcg
     3961 gcaggcacca gggccgtggc cgaagcggcg gccgatgcga ccgcaagggg tgcaatcagc
     4021 gtcatcgggg gtggcgactc cgcggctgcc gttgcccaga tggggcttga cgaccggatg
     4081 acccacatca gcaccggtgg cggcgccagt ctcaaatacc ttgaagggaa ggccatgccc
     4141 cccatcgaag tccttgacag ggattgatac gccctcgacc cggttcctgc gtgactttcc
     4201 ggattagtgt aaaatgccag acatgaagat aaaaaccaga ttcatcctgt ctgcggtgtc
     4261 gccggaagcg tttccggctt ccccgcgtcc cgaggttgcc ttcgtcggac gatccaacgt
     4321 cgggaagtca agcctgataa acgcgctggt gggaacgaag atggccaggg tttcttcgag
     4381 acccgggctg acccaggcga tcaacttctt cgaggtcgat gtggccgcgg tgcccgggct
     4441 ttcggtctcg aggccgggca cctttttccc gatgatgctg gccgacctgc caggctatgg
     4501 ctacgcccag gtgtcccgaa aggaatcgcg gggatgggcc tcgttcatcg acccgtacct
     4561 gtcagcccgc gatcagcttg tccttgtggt ggtgctggtt gattcaacca tccccatcca
     4621 gccaggcgac atggagatgg ttcagtggct gcgcacgatg aatcggccgt tcctgattgc
     4681 ggcaacaaag ctggacaaga tcgggcccag ccgccgcctt gccgagacca acaggcttgc
     4741 cgccggtttt gaggcgcggg ttcttggagt aagcgcgacg tcgggcgacg gcctggaaga
     4801 attgtggaag atcgttctcg ccaattgcaa aaagacgcaa tccgccaagt caccgtgaac
     4861 cgatggtaat cgtccttccg gccgaatatg ccgcgaactc cggcgcgtac atcggctgga
     4921 tggtggccgg gccggccttg taggttccgg ccagggccgc gcggataccg taacggaacg
     4981 tgtattcgcc gcgcggcagc ctgtcgaaga agaaattctg gccgctgtcg cgaacctctt
     5041 cgtaccacgc gatgccaaga tcccagcgat agccggaatt ctgggtcgca ggttcgaatc
     5101 cggccggccg cggatcgcga aggtggacat attcaagcgg ctcgcccgac ttgattgaga
     5161 cctgaacctg aacctcgtca cccgggacca ccacggttcc atccgcaagc ggctcgagca
     5221 tcacaccgga agcggtcttc ttcttcagga agtacctgcg ttcgatgttc agcatgtcgc
     5281 catcgccctt gtcgggcaga gtgtcggtgc tgaaatgcca ggttgcggat gcaagcgccc
     5341 atcccggggt cttcgttgaa accgaaaccc tggacatcga ttcctcgacc atgtcggggc
     5401 cgtcgataac catctgcgcc gtcgtgtcgg cggacgcttc ggggtcgaac gtgaagtcgc
     5461 gttcaagttg tcccgccttg accgaaatga cctgtcggcc accgatttcg ccccgtgccg
     5521 acatgtggcg ggccagggcg tatatgacct ctgccgttgc cctggtcgat ttccagtgat
     5581 tcatcttctt gttgatcagc agccatagca ccaggtcgtc aatcgacttg tcttccggat
     5641 ccagttccat catcgtccgt attgcccggg cgtggttttc gatgctgtcg ttgtaccaga
     5701 gccacgaacg ttcctcctgg gcccagaaca tgccgcgatc gggctccgtc ctcgctccgt
     5761 ccatcaccga tgcccagaca agcctggcgt cgtcttttct ttcggcccgg tgaagcgtca
     5821 tggccagcat ggacttcatg cccatcgaca tgtccttcca gtgcgtaaac gccagttcca
     5881 gaaggttcct gcgttcgtc
//
LOCUS       DAOPPA010000173         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_14819_length_5886_cov_4.188990, whole genome shotgun sequence.
ACCESSION   DAOPPA010000173 DAOPPA010000000
VERSION     DAOPPA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14819_length_5886_cov_4.188990"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1345)
                     /locus_tag="PKH54_06575"
     CDS             complement(98..1345)
                     /locus_tag="PKH54_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99589.1"
                     /translation="MSDKTLYPRFLRSRVEDALTDSPVVLIHGPRQCGKTTLARMVGD
                     DSGYAYLTFDDDVQRSSARTDPVGYVADLPDRVILDEVQRVPELFTAIKVAVDSRRKP
                     GSFILTGSANVLLVPRLADSLAGRIEILRLHPLSRAELVGRACNFLATLFGAGFKAGQ
                     SGQRLGNVLAEHIVGGGYPAALARATCHRRALWYRDYADTLIQRDIQDLARIRALDAL
                     PRLLALAAGQTASLINVSELAAPFQVSRQTIREYLTLLSRIFLLEELPPWHSNQLKRL
                     VKTPKLHIGDTGLACALMGVNPGSLWMDRPLFGRLLESYVYQELRRLASFRDESTAFY
                     HFRDKDQVEVDLVIESEGLVAGVEVKAASTVTTADFKGLRKLKDAAGSRFVCGVVLYD
                     GSAVAGFGEGLYAVPVSRLWSDE"
     gene            1569..4214
                     /locus_tag="PKH54_06580"
     CDS             1569..4214
                     /locus_tag="PKH54_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99590.1"
                     /translation="MNRVTILSRTILMVLGAGALVMAAGCGDSGTPDGDADVVAADVA
                     ADTADVAAPDTTDTTQGDMVGDSDDDVAQDAAEDSADGSMGDAGDVAGDLADDIPGVD
                     VVNPDEIFQPIQDREFAQEINHTGIEIDRRITDIVDILIKPAGNTLPFDDVLVLTSDG
                     VWLRDPVAPAEPGTWEPVKIAVNPTYGPVLGGAYTDEYVLVVQKTALLKLINNDDAQP
                     ELKPWMILADFQMMSVTQAYGWIIVADQFNVALFYGDQTRLGITGTTLAVKFATINAD
                     HSRIYVAGANGDGAEVIQVWQYNIQSDRFDVLIATVPSPVNDIRQMIADVAVPSELEL
                     VVVGQDGIAGYAAIESDEQAPAEIPVFAANRVPLNGATDIAKTADGGFIVTTMGGAMR
                     MIDRGDGPEWRFYNTLRWVADNDVRAVATTPDVPDSPIYFGSAAGMTWTTVERMTIED
                     KMALLREKIELRHDRDGAVADSRLTVPGDLSTSIPWDSDNDGGWTSYWLIAECMRWKE
                     TNDPQAKANFDKSLQAMLNLRLLTGLDWFLARALIRKDGCRLDDCDNPDDGMWYTSPD
                     GEWWVKSDTSNDEVTSHLFMMGPAYDLCADEIQKKAIAAHVSNIIGGIIDHDYQLLRP
                     TGECTTYGQFDPGYVNFIGLFGDGGQRSVQTLAAMNLAIYMTADQESRLQPGETLDMP
                     GSQKFIDAKAFLMNDENHYDDNVMTSTEPPGRHGNGDGDELSTQAFFILVQYEQDAAL
                     KARWLKGWRSVYDNLKTQQSAVWDVIQGVITQTVPNLYLGERWFRLYPLDLIRWNIHN
                     NHRLDLVKAPQYYIDRPEFRERYMRSDNRIIPSDERPNDRHNTSQFQIEGGWGDNVEM
                     DAADVLAAYWMARYYGFIEPADQVE"
     gene            complement(4186..5352)
                     /locus_tag="PKH54_06585"
     CDS             complement(4186..5352)
                     /locus_tag="PKH54_06585"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99591.1"
                     /translation="MMIPRFFDDLGQYLAPGKVLVIFGARQVGKTTLVRNFLRRSLLK
                     FRFDSGDDIRIQELFAAGRLDRLQEYAQGFDLVVLDEAQRVPGIGLGLKMIVDAMPGL
                     QVIATGSSSFDLAGQVGEPLTGRKRTLNLFPVAQLELATMHNRFDLRGRLDEYLIFGG
                     YPEVVAAQGRDEKIRLLNEIASSYLLKDVLALNNIRNSRSIFDLLRLLALQVGSEVSL
                     TELGTQVGISYKTVDRYIDILEKSFILMRLPGLGRNPRTDLRKKNKYYFMDTGIRNAL
                     ISNFNPTDLRADCGQLWENFLCVERMKLLSYTGRHANMYFWRTNQGSEIDLVEEAGGG
                     FAGFEFKWGVKGRSRPRDWTEFAPNGSWTVVNRDNYLDFVLETSLFTPPGQRAR"
     gene            complement(5591..>5886)
                     /locus_tag="PKH54_06590"
     CDS             complement(5591..>5886)
                     /locus_tag="PKH54_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99592.1"
                     /translation="CAITWTVDGKDIVGTASGEGTEQDQVTDTFVVAEGDDSFDLSGV
                     PRGPIEVSIDGVLCSDNWIVEFPDYNPRAVPGEGCIPEIGDTVTITYSVSCIG"
BASE COUNT         1322 a         1763 c         1677 g         1124 t
ORIGIN      
        1 tatcgatgat cctgatcaat gtcgagacga gacggtctat cccctgtgcc cctgggaatc
       61 gagggagccc tatttttgaa acaggattga cagcacctta ctcgtcactc cagagcctgg
      121 aaaccggaac tgcgtaaagt ccttcaccaa agcctgcaac ggcagaaccg tcatacagca
      181 caacaccgca aacaaagcga cttccagcgg catccttgag cttgcgcagc cctttgaaat
      241 ccgcggtcgt caccgttgat gccgccttga cctcgacacc ggccaccagg ccttccgact
      301 cgatcacaag atccacttca acctgatcct tgtcgcgaaa gtgatagaag gcagtggatt
      361 catcacggaa agacgccaga cgccgcaatt cctgatatac gtaactttcc agcaacctgc
      421 cgaacaacgg acgatccatc cacagcgaac caggattgac acccataagt gcgcaggcca
      481 accccgtatc accgatgtgc agctttgggg tctttaccag gcgtttgagt tgattgctgt
      541 gccagggcgg caattcttcc agcaaaaata tccgtgacaa cagggtcaga tattcacgga
      601 tggtctggcg gctgacctgg aacggggccg cgagttcgga cacgttgatc aggcttgcgg
      661 tctgccccgc tgccaatgcc agcaggcgcg gcaaggcgtc gagcgccctg attcgggcca
      721 agtcctggat atcgcgctga ataagtgtat cggcataatc ccgataccaa agggcccgtc
      781 ggtggcaggt tgcacgggcc agggcggccg gatagccgcc accgacaata tgttcagcca
      841 gtacatttcc cagacgttgc ccggactgtc cagccttgaa gccggccccg aacaatgttg
      901 ccaggaagtt gcaagccctt ccgaccaatt cagcccggga cagcggatgc agccgaagga
      961 tttcgattct tccagccagg gagtccgcaa gacggggaac cagaagaaca ttggccgatc
     1021 ctgtaaggat gaatgacccc ggcttgcggc gactgtccac ggcaaccttg atggcagtaa
     1081 acagttccgg gactcgttgt acttcatcga gtatcaccct gtcgggcaaa tcggccacat
     1141 atccaacggg atccgttctg gccgacgaac gttgaacgtc atcgtcaaaa gtcaggtagg
     1201 cgtatcccga atcgtccccc accatgcggg ccagagtggt cttgccgcac tgcctggggc
     1261 cgtgaatcag gacaaccggg gaatccgtca gcgcatcttc aacccgtgaa cgcagaaatc
     1321 tgggatacaa cgtcttatca gacattaatc cactaccacc aaaagccttg tctgattatg
     1381 gccttagata cgtccaattg caagcctcag ttcgtccaat tgcaagtttc gattcgtcca
     1441 attgcaagat gtcggcgtgg gtcatcgaaa tcagggaagc cgattatgtc tcgtcgggtt
     1501 tcgtgcaccg gggatggcgg cgcaatccgt tacatgaaac aatcaggcgt cttccagggg
     1561 ggttccagat gaaccgcgtg acgatccttt ccagaacgat acttatggtg ctgggggcgg
     1621 gggccctggt gatggcggcc ggatgcggtg attcagggac ccctgatggc gacgcggacg
     1681 tggtggccgc cgatgtggct gccgacacgg cagacgttgc ggcgccggac accaccgaca
     1741 cgacccaggg cgacatggtt ggcgactccg atgacgacgt tgcccaagac gcggcggaag
     1801 actccgctga cggctcgatg ggcgatgcgg gcgacgttgc gggcgacctt gcggacgaca
     1861 tccccggcgt tgatgtcgtc aaccccgatg aaatcttcca gcccatccag gatcgcgaat
     1921 tcgcccagga aataaaccac accggcatcg agatcgaccg gcgaatcacg gacatcgtcg
     1981 atatcctgat taagcccgca ggaaacaccc tgccctttga cgacgtgctg gttctgactt
     2041 ccgacggcgt gtggctgcgc gacccggtgg ctccggccga acccggcaca tgggagcccg
     2101 tgaagatcgc ggtgaacccg acgtacgggc cggtgctggg cggcgcctat accgacgaat
     2161 atgtcctggt ggtccagaag accgcgctgt tgaagctgat caacaacgat gacgctcagc
     2221 ccgagcttaa accatggatg attctggccg atttccagat gatgtccgtg acccaggcgt
     2281 acggctggat catcgttgcc gaccagttca acgtcgcctt gttttatggc gaccagacgc
     2341 gcctgggcat caccggcacg actcttgccg ttaaattcgc caccatcaac gccgaccaca
     2401 gcaggatcta tgtggcggga gccaacggcg atggcgccga agtcatccaa gtctggcagt
     2461 acaacatcca aagcgacagg tttgatgtcc tgattgcgac cgtcccgtcg cccgtgaacg
     2521 acatccggca gatgatcgcc gacgtcgccg tcccgtccga actcgaactg gtggtcgtcg
     2581 gccaggatgg catcgccgga tacgcggcca tcgaatccga cgaacaggct ccggccgaaa
     2641 tcccggtatt cgcggcaaac cgtgtgccgc ttaacggcgc gaccgacatc gccaaaacgg
     2701 ccgacggcgg attcatcgtc acgaccatgg gcggcgcgat gcgcatgatc gaccgcggcg
     2761 acggccccga gtggcgtttt tacaacacac tgcgctgggt ggcggacaac gacgtgcgcg
     2821 cggtcgccac gacgcccgac gttccggact cgcccatata cttcgggtcg gcggccggca
     2881 tgacctggac cacggtcgag cgcatgacga tcgaagacaa gatggccctg ctgcgtgaaa
     2941 agatcgaact gcggcacgac cgcgacggtg cggtcgccga cagccgactg accgtcccgg
     3001 gcgacctttc gaccagcatc ccgtgggact cggataacga cggcggctgg accagctatt
     3061 ggctgattgc cgaatgcatg cgctggaagg aaaccaacga ccctcaggca aaggcgaact
     3121 tcgataaaag cctgcaggca atgctgaacc tgcgcctgct gacaggcctg gactggttcc
     3181 ttgcgcgggc gcttatccgc aaggacggct gcaggcttga cgactgcgac aacccggacg
     3241 acggcatgtg gtacacgtca cctgacggcg aatggtgggt gaaatcggac acttccaacg
     3301 acgaagtcac gtcacacctg ttcatgatgg gcccggcgta cgacctgtgc gccgacgaaa
     3361 tccagaagaa ggccatcgcg gcgcacgtca gcaacataat cggcggtatc atcgaccacg
     3421 attaccagtt actgcgcccg accggcgagt gcacgaccta cggccagttc gacccgggat
     3481 atgtcaattt catcggactg ttcggtgacg gcggacaaag gtcggttcag acgctggcgg
     3541 cgatgaacct ggcgatttac atgacggccg accaggaatc caggctgcag ccgggcgaaa
     3601 ccctggacat gcctggcagc cagaagttca tcgacgccaa ggcgttcctg atgaacgacg
     3661 aaaatcatta cgacgacaac gtcatgactt cgaccgagcc gccggggcgc cacggcaacg
     3721 gcgacggcga cgaactttcg acccaggcgt tcttcatcct ggttcagtac gaacaggacg
     3781 ctgccctgaa ggcccgctgg ctgaagggct ggcggtcggt ctatgacaac ctgaagaccc
     3841 agcagtcggc cgtctgggac gtcatccagg gcgtgattac ccaaaccgtt ccgaacctgt
     3901 acctgggcga aagatggttc cgcctttacc cgctggatct gatccgctgg aacattcaca
     3961 acaaccatcg gctggatctg gtcaaggcac cgcagtacta catcgaccgc ccggaattcc
     4021 gcgaaagata catgcgcagc gacaaccgca tcatcccgtc ggacgagcgc ccgaacgacc
     4081 gccacaacac cagccagttc cagattgagg gcggctgggg cgacaacgtc gagatggacg
     4141 ccgccgacgt ccttgccgca tattggatgg ccaggtatta cgggtttatc gagcccgctg
     4201 accaggtgga gtgaacaggg acgtttccag cacaaaatcc aggtagttgt ccctgttgac
     4261 gacggtccac gagccgttcg gggcgaattc ggtccagtcc ctggggcgcg accgcccttt
     4321 gacgccccac ttgaactcga aaccggcgaa tcccccgcca gcttcctcga ccaggtcgat
     4381 ctctgatccc tgattggttc gccagaaata catatttgcg tgacggcccg tgtacgacag
     4441 aagcttcatt cgctcgacgc aaaggaagtt ctcccacagt tgcccgcaat cggctcgcag
     4501 gtcggttggg ttgaaatttg atatcaacgc gtttcgaatc ccggtgtcca tgaagtagta
     4561 cttgttcttc ttacgcaggt cggtccttgg attgcgcccc aggccgggaa gacgcatcag
     4621 gatgaatgac ttctcgagta tgtcgatgta ccgatcgacc gtcttatagc tgattccgac
     4681 ctgtgttccg agttcggtca aggacacctc gctgccgact tgaagcgcca gcagacgcag
     4741 aaggtcgaag attgaacgac tgttcctgat gttgttcagg gccagaacgt ccttcagcag
     4801 gtacgaggac gcgatttcgt tcagcaggcg tattttttca tcgcggccct gggcggcgac
     4861 gacttcggga tagccgccaa aaatcaagta ctcatcgagc ctgccccgca agtcaaaacg
     4921 attatgcatc gtcgccagtt caagctgggc caccgggaac aggttcagcg tgcgcttgcg
     4981 gccagtaagc ggctcgccga cctgccctgc cagatcaaaa gacgaagacc ccgtcgcgat
     5041 cacttgcagt cccggcatgg cgtcgacaat catcttcagg cccagtccga tcccgggcac
     5101 gcgttgtgcc tcgtcgagca cgaccagatc gaagccttgc gcgtactcct gcaggcgatc
     5161 caggcgacca gccgcaaaaa gttcctggat gcgaatgtcg tccccggaat caaaacggaa
     5221 cttcagcaac gacctgcgaa gaaaattcct gaccaacgtc gtcttgccga cctgccgggc
     5281 accaaagata acaagtactt ttcccggcgc cagatactgc ccaaggtcgt cgaaaaacct
     5341 gggaatcatc atgttcaaac ccccgaatcc aatgaacagt tgcattgtaa ctcttgaaaa
     5401 tatcaatttt caagaagcgg tactcctcga aattatccta tttcaagagg cagcgctcct
     5461 cgaaaactgc catttccaag gaattcttgg ggacggggtt aataaaacga aaaagtaatt
     5521 ccattttatg tacatattgc gaagacatat tttcatttga cggacttttc gggctggggc
     5581 gcggtgggaa tcagccgatg cagcttacgc tgtaggtgat ggtcacggta tcgccgattt
     5641 cggggatgca gccctcgccg ggaacggccc tgggattgta gtccgggaac tcgacgatcc
     5701 agttatcact gcacaggacg ccatcgatgg aaacttcgat tggcccgcgg ggaacgcccg
     5761 aaaggtcgaa gctgtcgtcg ccttcggcca ccacgaatgt gtctgtgacc tggtcctgct
     5821 cggtaccctc gccgctcgcg gtgccgacga tgtccttgcc gtcaacggtc catgtgattg
     5881 cgcagg
//
LOCUS       DAOPPA010000174         5813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15069_length_5813_cov_3.702154, whole genome shotgun sequence.
ACCESSION   DAOPPA010000174 DAOPPA010000000
VERSION     DAOPPA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15069_length_5813_cov_3.702154"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1775
                     /locus_tag="PKH54_06595"
     CDS             282..1775
                     /locus_tag="PKH54_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOC99593.1"
                     /translation="MGLPYFKPFVPDAARTKTVLKLAGPVMAANLTQTLINQFDTILV
                     GHLPKEYSIAGQAALGYSVLLHWMVGGFVGAISVGTQAMTARRLGEDNRPGAGGVLFN
                     SIMLALMAGTFVTVLAIVFTRPLFALFTSNEQVLALGVPYTQARFAGILSMVGTLSFK
                     AFFDGLGKTHYHLMVAVGMNVVNAVLAYMLVFGFWGIPGMNVVGAGIAATVASYFGLF
                     GMLAFALKTSHRKDFNIFRASNFRWKTIGGIARLSVPSGIATVFVMTGFLLFFKWVGM
                     LDEAVMSGPLDPMDTASIAWLGSFSGLPVDSTTFESLVNIHAPVNEAATKVIIDILFL
                     SIMACMGLGIATATLVSQNMGRRQPEEAEKYGWTAVRIGAWFAGILGVAALIWPDAAM
                     GIFTQDPEVVEVGRTPLRIVAAGEFAIGFGLVLAQALFGAGASRFVMKVEIALHVGVL
                     VPLSYVLAIPLGLKLNGIFIAAIVYVVLLALFMTLKFRSGTWKSIRI"
     gene            complement(1792..2859)
                     /locus_tag="PKH54_06600"
     CDS             complement(1792..2859)
                     /locus_tag="PKH54_06600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC99594.1"
                     /translation="MSINKSAVLLFAAALAISFSAVGLEQAAEGSKAASQGFVVDTVV
                     ATVGTDVITQYDVKKRMQITSTPLASIMAGGTGMEDESAQFKQALDDLISDMLVLKEA
                     RKSGITVPREEVDAHVDQLKKRNNWTDSDFAVAVGMLGFEGVDAYRAHASNELLKSKV
                     LRMKIGGRISVSDREVQEIFDARYEGGKTEEEIHLAHIAIPIPDDVTDDGIKDLLRQA
                     SEIRKIAARGDKPFDELARTFGRDSTAARGGDVGWFPVGRLQPSLEEAAFGLRDGEIS
                     DVVQSSFGFHILTVLERRRVPLRDADEARRRIKWELSEVAFQKGYKDFVMELRRSARI
                     NVMSAVARPAATDGKASPDRQ"
     gene            complement(2856..3926)
                     /locus_tag="PKH54_06605"
     CDS             complement(2856..3926)
                     /locus_tag="PKH54_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC99595.1"
                     /translation="MWVNIRPGRWSGGNLDIKNINWNKSIAVIGPVVCIIAASGCRPE
                     RNLSESVEAVRSKVSTRSGERVDSPVVATIGDREIRFDEVVANLDSLPVFVRMRYKSP
                     ERRLEFLEAFVEFQVLALAAAAEGLGSDPKVVDVLKRDLASRYLIENFDLKQRTTDIS
                     EDVIREFYDSHPWLFSHPAQNHVFRILCSDRAQAAKVAYRVRSLVESATGDPLDVFRE
                     HVARSSVDPAAFRTSGDIGIYPSAGGSSASVPVQVAAAAEALDTLHQVSDPVEAPDGW
                     SVLFLARQIPPVSKDIDQARAEIAGMILDARRLEARRALADSLIRPLKESGSLVVNHE
                     LLSDLAAADIAARKSPGPEKSR"
     gene            complement(3938..>5813)
                     /locus_tag="PKH54_06610"
     CDS             complement(3938..>5813)
                     /locus_tag="PKH54_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC99596.1"
                     /translation="PVDRANLLEKYTPPSEGQSRQLDRLGTQAWAARKKQARSAARDI
                     ADNLRKLYARRMASTAPSMSAPDAEFREFEATFQWETTPDQDKAIAEILEDLQNRTPM
                     DRLVCGDVGFGKTEVAIRAAFKAISDGYQVAVLVPTTILAEQHRLTFAARFRNTPAVI
                     ESISRFKSPREIRQTLQALRTGGIDVIVGTHRLLSADVQFMNLGLLIIDEEQRFGVAH
                     KERLREMAAGVHTMSLTATPIPRTLHMALSGIRELSLITTPPRDRLAVRTFVARSGMG
                     LIRSAILRETARGGQVFVVHNRVEDIHELAASISVAVPEARICVGHGQMSGQELEAVM
                     SGFVRGDFDVLVATTIVESGLDIGTANTIIIHDADRLGLAQLYQLRGRVGRSSEQAWC
                     YLLVRDPGRLSGEARMRIEAIERFSELASGFNVASMDLAIRGSGEILGAEQSGHLESV
                     GEEMFLEMVREAMDELSGEDSGEKPEVDMKVDVEARLPAQYLPDEKLRLRFYRRLSSA
                     SEPSDVDAIAAELADRFGPLPDQARNLVAILRLKIVAAAVGLVALAIRGDDLNMAVGG
                     GGAGTLASIAAAFESAGFVREPGRIVERVRFVFPGAAGARMAGISRVEMVEMVLGRLV
                     "
BASE COUNT         1128 a         1872 c         1650 g         1163 t
ORIGIN      
        1 cccgcgacct gctgattaca agtcagctgc tctacctgtt gagctacgcc agcgctgaaa
       61 agaacgtgcc cgtcaaaaag gccacttatt atctgggaga ctccctcggc tgtcaataca
      121 aaaattgacg ccgcaaggaa cctcggatgt cttttcgacc ggaattccgg gcgaacgggc
      181 gatattttga tccaaagcat ccgacaggtc aagccatggc ggacgttacg ggtcgatttt
      241 gcttgcgttc gagtacccgg tcagattagg ctccacacgc catgggcttg ccatacttca
      301 aacctttcgt tcccgatgcc gccaggacga agaccgtcct gaagctggcg gggccggtaa
      361 tggccgccaa ccttacccag acgctcatca accagttcga cacaatcctg gtcgggcacc
      421 tgcccaagga atattcaata gccggtcagg cagccctggg atacagcgtg ctcctgcact
      481 ggatggtggg tggctttgtc ggcgccatct cggttggaac ccaggccatg acggcaaggc
      541 gcctcgggga ggacaatcgc cccggtgccg gaggcgtcct gttcaattca ataatgctcg
      601 cgctgatggc cggaaccttt gtaacggttc tcgccattgt gttcacgcgc cctcttttcg
      661 cgctgttcac cagcaacgag caggtactcg cgctgggggt tccgtacacc caggctcgct
      721 tcgcgggcat cctgtcgatg gtcggaacgc tgtctttcaa ggcgttcttc gatggactcg
      781 gcaagacaca ctaccatttg atggtggctg tcgggatgaa cgtcgtcaac gccgtgctcg
      841 cgtacatgct ggtctttgga ttctggggca tccccggcat gaacgtcgtc ggcgccggca
      901 tcgccgcgac ggttgcaagc tatttcggtc ttttcggcat gctggcattc gcgctgaaga
      961 catcccaccg caaggatttc aacatcttcc gggcgtccaa cttccgctgg aagacgattg
     1021 gcggcatcgc caggttgtcg gttccgtcgg gaatcgccac agtgttcgtg atgaccggct
     1081 tcctgctgtt cttcaaatgg gtcggcatgc ttgacgaggc cgtgatgtcg gggccgctgg
     1141 atccgatgga cacggcttcg atcgcctggc tcggctcgtt ctccggcctt ccggtcgatt
     1201 caaccacctt cgagtccctg gtcaacatcc acgcgccggt gaatgaagcg gcgaccaagg
     1261 tcattatcga catactgttc ctgtcgatca tggcgtgcat gggactcgga atcgcaacgg
     1321 cgacgctggt cagccagaac atgggcaggc gccagccgga agaggctgaa aaatacggat
     1381 ggaccgccgt caggatcggc gcctggttcg ccggcatcct gggtgtcgcc gcgctgatct
     1441 ggccagatgc cgcaatgggc atcttcaccc aggatcccga agtcgtcgag gtcggccgca
     1501 ccccgctgcg catcgttgcc gccggcgaat tcgcaatcgg attcgggctt gtgctggccc
     1561 aggcgctgtt cggcgccggg gcttcgcgct tcgtcatgaa ggtcgaaatc gccctgcacg
     1621 tgggcgttct ggttccgctt tcctatgtac tggcaatccc gctcggcctt aagctcaacg
     1681 gcattttcat cgccgccatc gtttacgttg tcctgctggc tcttttcatg acgctgaagt
     1741 tccgctcggg aacctggaag agtatcagaa tctgatcgaa ttgcggtgac gtcactgcct
     1801 gtccggcgaa gccttcccgt ccgtcgcggc cggcctggcg actgcgctca tcacgttgat
     1861 ccgcgcgctg cgcctgagtt ccatcacgaa atccttgtat cctttctgga acgcgacttc
     1921 ggaaagctcc catttgatgc gccgccgtgc ctcgtcggcg tcacgaagcg gcaccctcct
     1981 gcgctcgagc accgtcagga tgtgaaagcc gaagctggac tgaaccacat ccgaaatctc
     2041 gccgtcgcgc agcccgaaag ccgcctcctc cagggagggc tgaaggcgcc cgaccgggaa
     2101 ccagccgaca tcgccgcccc tggccgcagt tgaatcgcgc ccgaatgtcc gtgcaagttc
     2161 atcgaacggc ttgtcgcccc gggcggcaat cttccggatt tcggatgcct gccgcagcaa
     2221 atccttgata ccgtcgtccg tcacatcgtc cggaatgggg attgcgatgt gcgcaaggtg
     2281 gatctcctcc tcggtcttgc cgccctcata cctggcgtcg aagatttcct gcacctcgcg
     2341 gtctgagacc gaaatccttc cgccaatctt catgcgcagc acctttgact tcagcagctc
     2401 gttggacgca tgggcacgat acgcatcgac tccctcgaaa ccaagcatcc ccacggcgac
     2461 ggcaaaatcg gaatcggtcc agttgttcct cttcttcaac tgatcgacat gggcatcgac
     2521 ctcttccctg ggaacagtga taccggactt gcgcgcctcc ttcagcacga gcatgtcgga
     2581 aatcaggtcg tcaagggcct gcttgaactg ggccgactcg tcctccatcc cggtgccgcc
     2641 ggccatgatg gaggccagag gcgtcgatgt aatctgcatc cgcttcttga cgtcatactg
     2701 cgtgatcacg tcggttccca cagtggcaac gacggtatcg acaacgaagc cctgggacgc
     2761 ggccttcgaa ccttcggccg cctgctcaag gccgacagcc gaaaaggaaa tggccagggc
     2821 ggcggcaaac agcagaacgg cactcttgtt gatactcacc tggacttctc cggacccgga
     2881 ctcttccggg cagcaatatc ggccgcagcc agatcggaca acagttcgtg gttcaccacc
     2941 agcgaaccgg actccttcag cggacgaatc agtgaatcgg ccagggcgcg ccttgcctcc
     3001 agcctgcggg cgtcaagaat cattcctgcg atctcggccc tggcctggtc gatgtccttc
     3061 gataccggcg gtatctggcg cgccagaaac agaaccgacc agccatcggg cgcctcgacg
     3121 gggtcggaca cctgatgaag cgtgtcaagc gcctcggcgg ccgccgcgac ctgaaccgga
     3181 acggacgcgg aagatccgcc cgcggacgga taaatgccga tgtcgcccga cgtcctgaac
     3241 gccgcgggat caacggaaga cctggcgaca tgttccctga acacgtcaag cgggtcgccg
     3301 gttgccgatt ccacaagaga tcgaaccctg taggccactt tggcggcctg ggcccggtca
     3361 ctgcacagaa tcctgaaaac atgattctgg gcagggtgcg aaaaaagcca cggatgggag
     3421 tcgtagaact cccttatgac gtcttcggaa atgtctgtgg tcctctgctt cagatcgaaa
     3481 ttctcgatca gatagcgcga cgccagatcc cttttcagga catcgaccac cttcggatcg
     3541 gatccaaggc cttcggcggc cgcagcaagg gccagaacct ggaactcgac aaaggcttcc
     3601 aggaattcca ggcgacgttc cggcgacttg tacctcatcc tgacgaatac cggcaacgag
     3661 tcgaggttcg cgacaacctc gtcgaaccgg atctcccgat ccccgatcgt ggccaccacc
     3721 ggtgaatcga cacgttcccc ggagcgcgtc gaaaccttgc ttctcacggc ctcaaccgac
     3781 tctgaaagat tccgctcggg cctgcatccg gaagccgcga tgatgcagac caccggcccg
     3841 ataaccgcga ttgacttgtt ccagttgata ttcttgatgt ccaggttccc gccagaccac
     3901 cggccaggcc gaatgttaac ccacaactag cacccaatca aaccagccgg ccaagaacca
     3961 tttcaaccat ctcgacacgc gatatgccgg ccatgcgtgc gccggcagcc cccgggaaga
     4021 cgaatcggac acgctcgacg attctgcccg gttccctgac gaaacctgcc gactcgaatg
     4081 cggcggcaat cgatgcaagt gtcccggcac ctccgccgcc gaccgccatg ttcaggtcgt
     4141 cccctctgat tgcgagcgcg accaggccga ccgcggcggc aacgatcttc aacctgagga
     4201 ttgcgacaag attccgggcc tggtcgggaa gcggcccgaa tcgatcggcc agttctgcgg
     4261 caattgcatc gacgtcggat ggctcggacg ctgatgagag cctcctgtaa aagcgaagcc
     4321 tcaacttctc gtccggcagg tactgggccg gaagccttgc ctcgacatcg accttcatgt
     4381 cgacttcggg cttctcacct gagtcctcac ctgaaagctc atccatagcc tcgcgaacca
     4441 tttccaggaa catctcctcg ccaaccgact ccagatggcc ggactgctcg gcgccgagga
     4501 tctccccgct tcctcgaatc gccagatcca tgctggcaac gttgaatccg cttgccagct
     4561 cgctgaacct ctcgattgcc tcgatccgca tccgcgcctc gccgctcaat cgaccgggat
     4621 cccggacaag caggtaacac catgcctgct cgctggatcg gccgacccgt ccacgaagtt
     4681 gataaagctg ggcaaggcca agcctgtcgg catcgtggat gataatcgtg ttggcggttc
     4741 cgatgtccag gcctgactcg acgatggttg tcgcgacgag cacgtcgaaa tcgcccctga
     4801 cgaaaccgga catcacggcc tcaagttcct ggcccgacat ctgcccgtgg ccgacgcaga
     4861 tcctggcctc gggcactgcc accgatatcg aggcggcaag ttcgtgtatg tcctcgaccc
     4921 ggttgtgcac gacaaacacc tggccgccgc gcgccgtctc ccgcagaatc gcggaccgta
     4981 tcagtcccat tcccgaccgt gcgacgaacg tcctcacggc caggcgatcc ctgggaggcg
     5041 tcgttatcag cgagagctcg cgaattcccg aaagggccat gtggagcgtc ctgggaatcg
     5101 gcgtggcggt aagcgacatc gtgtggactc cggcggccat ctcccgaagc cgttccttgt
     5161 gggcaacgcc aaatctctgc tcttcatcga ttatcagcag gccaagattc atgaactgca
     5221 cgtcggccga cagcagacgg tgcgtaccaa caataacgtc gattccgccg gttcgaagcg
     5281 cctgaagtgt ctgcctgatc tcgcgcgggc tcttgaagcg gcttatcgat tcaatgactg
     5341 ccggggtatt ccggaacctg gcggcgaacg tcagcctgtg ctgttcggca agaatcgtcg
     5401 tcggcacaag aaccgcaacc tggtatccgt ccgaaatggc cttgaaagcg gccctgatgg
     5461 ctacctcggt cttgccgaaa ccgacatcgc cgcagacaag ccgatccatg ggcgtacggt
     5521 tctgcagatc ctcaaggatt tccgcgatcg ccttgtcctg atccggcgtg gtctcccact
     5581 ggaaagtcgc ctcgaactcg cggaactcgg catccggggc cgacatggat ggagcggtcg
     5641 atgccatcct ccttgcgtac agcttgcgaa gattgtccgc gatgtcccgg gcggcgctgc
     5701 gcgcctgctt ctttctggcg gcccatgcct gggttccaag acgatcaagc tgcctggact
     5761 gaccttcgga aggcggggta tacttttcca gcaggttcgc ccggtcaaca ggt
//
LOCUS       DAOPPA010000175         5800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15105_length_5800_cov_3.573020, whole genome shotgun sequence.
ACCESSION   DAOPPA010000175 DAOPPA010000000
VERSION     DAOPPA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15105_length_5800_cov_3.573020"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKH54_06615"
     CDS             <1..361
                     /locus_tag="PKH54_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99597.1"
                     /translation="RFGIGFGLSTEIRGFEIGFSYAHVFHMPRNVTNGGITQQVIDTV
                     ASEDGTKSVTRPGYVVNNGEFTNSIDVFTFGIGYHHKPKAKRDFPKDPVPESETADEA
                     VEEGPEPSPEDPADPSV"
     gene            471..617
                     /locus_tag="PKH54_06620"
     CDS             471..617
                     /locus_tag="PKH54_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC99598.1"
                     /translation="MGMQVTTTIDHELCIGCGRCIKVCPQDTISLIEGKAVVTGRMSL
                     NCGH"
     gene            750..1358
                     /locus_tag="PKH54_06625"
     CDS             750..1358
                     /locus_tag="PKH54_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC99599.1"
                     /translation="MASRRSTRNYMDTPVPMDVLHDLVKVGCLAPSGTNCQLWKFGII
                     PDREGMLKIARLTMDFFSRTNRLAANPLVRLFVPQLREYHRLYAQAVEESIREFKAGG
                     RESLFHGAPAAITISSKPGASCPAEDALLASQNILLAAHSMGYGTCLIGMLVEAVRHD
                     RAIRDALRVLPGERLHSVIVVGVPNEDWKRPCGRMKPEIFNV"
     gene            1383..2087
                     /locus_tag="PKH54_06630"
     CDS             1383..2087
                     /locus_tag="PKH54_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC99600.1"
                     /translation="MPDMNTPRYGAIFDLDGTLVASEVLYQRATGFILKKAGRSLSEL
                     TPQEKAAIPGRSAIRNMRFYCERFELPWSPEFLVEKRMEIVAGLVREEGVMLIPGAME
                     FCEMLRAEPFRMAVASSSPGPYVRLVLETTGLAGLFDAVLSGDDVTAHKPDPEIFLTA
                     AARLECPPRSCLVFEDADSGLQAAKAAGMKSILIDNETILPEQKLGATRVIPHYCGLG
                     ISEVKRVINGGIILLD"
     gene            2144..3577
                     /locus_tag="PKH54_06635"
     CDS             2144..3577
                     /locus_tag="PKH54_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC99601.1"
                     /translation="MATRELPRTLSLTTATAIVIGSVIGSGIFKKCSSMTDALPSPTL
                     VLLVWVLAGVVSLIGALCAAELTAAWPDAGGLYAHLRRALGPFWGFLYGWSTLSVIQT
                     GSIASIAYVFAEYLRFFGGWGDVPPELAAWGVNLFGVIDIYPLRDLGTKAVAVSCITG
                     LTVVNIVGVRPGAAIQNVFMYLKFAIMFGIIGVAAFGAGHLLSAVSGPIFPQANGLQA
                     TSVEPSFTMLAGGFAIALSGAFWAYDAWICATYVAAEVKRPERNMPIALVAGMAVVCI
                     IYLLVNSAYFHLLTVDEIRNSTLVASDALGRVLPLAGALVAAAVCLSTFGTINGTALS
                     SARVYYAMARDGLFHERLADVHPRRQTPHVALIVQGIWSSLLVFSGTFDQITDMLIFV
                     SWGFYALLAVAVLVARRRFPDTPRPFKTPGYPWLPTIFIVFSVAYLIFSVAANTRNAL
                     MGTLLVLPGIPFYFHFKKHRSAAVPRP"
     gene            complement(3540..>5800)
                     /locus_tag="PKH54_06640"
     CDS             complement(3540..>5800)
                     /locus_tag="PKH54_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC99602.1"
                     /translation="LIGIISDVQYMRGGVLDDEAGVAFLRMVRSRNATLPVLLQSASP
                     ENRSKAESLGAHFVDKNSPSLTMELKDFIVTQLGFGDFIFRMPDGTEITSVSNISEFI
                     EALATVPDESLEWHARYNHYSGWLMAHGEIDFARRIQPLGMSNFDSVADVRRYLLEVF
                     GEVRRAANRGKVVDVIDWQVSNRAEIVRLSGGSMGGKGRGLAFLNSSLNMMAMSRRYP
                     GVDVSLPLTAFIGTAEYDDFIERNGIDGDPPDMTDDQIRTRFLQGSLGDELKRRLLEL
                     VQMIDVPLAVRSSGLLEDSQACPLAGVYRTVMIPNSASEIGQRCHELCKAVKLVFSSV
                     YESRARDFLAGSRFLPRDEKMAVIIQELVGRLHGNYFYPDISGVAQSYNFYPTGSLKS
                     SDGVAGIALGLGRTIVSGGRIFRFCPQFPGTDILTPGDLVKDSQKDFWALRLDATTDA
                     MEGDEVATMDRLGILEAERHGVLGLIGSRWDMDGDMLVPDLQGPGPRVLDFAPVIKYG
                     SFPLAAIVKDVLALGEEAFGLPVEVEFAANITPGPEGRREFFVLQIRPLAVNRVFVDV
                     PGRTRDSAPPEVILHTSGALGNGQIEGVTDVVLVDVEKFSTTATLAMRDEIARINAGM
                     RESGREYLLAGPGRWGSSDRFLGIPVKWSDISQAVAIVEMDLQDFRIESSQGTHFFHN
                     LVARNAGYMKVLWGRHDCWVDIDAVMKWEVVERTAHCIHARAPAPLTIRMDGRSGRAE
                     VTVGEPPPTDVF"
BASE COUNT         1185 a         1813 c         1615 g         1187 t
ORIGIN      
        1 acgcttcggc atcggcttcg gcctttcgac ggaaatccgc ggattcgaaa tcggcttctc
       61 ttacgcccat gtgttccaca tgcccagaaa cgtcacgaac ggcgggatta cccagcaggt
      121 aatcgacacg gtggcgtccg aggacggcac gaagtcggtc accaggcccg ggtacgtggt
      181 caacaacggc gaattcacca attcaatcga cgtattcacg ttcggaatcg gataccacca
      241 caagccgaaa gcaaaaaggg acttcccgaa agacccggta ccagaatcgg aaacggccga
      301 tgaagcggtc gaggagggcc ccgaaccgtc cccggaagac cctgccgatc cttcagtctg
      361 aaacggtttt gccccgaagt cccgcgtgac aacacacagc cctgatcgaa ccgctgtctc
      421 gacacaccgc gccaatgtat aatcctccgg gcctccttcc ggagaagaca atgggaatgc
      481 aggttacaac gacaattgac catgaactct gcatcggctg cggacggtgc atcaaggtat
      541 gtccccagga cacgatcagc ctgatcgaag gcaaggcggt cgtcaccggc cggatgtccc
      601 tgaactgcgg ccactgacag gcgatatgcc cgaccggggc cgccagggtc ggcgcgctcg
      661 atccggaaac gcacagcttc aagactttca cgatgacgcc cggcttcgtc ccattcgggg
      721 gcggcgccac cccggaactt gtctccctga tggcttcgcg ccgatcaacg cggaactata
      781 tggacacgcc ggttccgatg gacgtgctgc acgaccttgt caaggtcgga tgcctggcgc
      841 cgtcgggaac caactgccag ttgtggaaat tcgggattat ccccgaccgc gaaggcatgc
      901 tgaagattgc ccgcctgacg atggatttct tttcgcgcac aaaccgcctg gccgcaaacc
      961 cactcgtccg cctgttcgtt ccgcagcttc gtgaatacca ccggctttac gcccaggccg
     1021 tcgaggaatc gatccgggaa ttcaaggccg gcggcaggga gtccctgttt cacggcgcac
     1081 cggccgccat aacgatttca tcgaaaccgg gcgcgagttg tccggccgag gacgcgctgc
     1141 ttgcatccca gaacatactc ctggccgcgc actcaatggg ttatggaaca tgcctgattg
     1201 ggatgcttgt tgaagctgtc aggcacgacc gggccatcag ggatgccttg cgcgtgcttc
     1261 ccggcgaacg gttgcactcg gtaatcgtgg tcggagtccc gaacgaggac tggaaaaggc
     1321 cgtgcgggcg catgaagcct gaaatcttta acgtttgacg ccaccttgcc gccgcgatta
     1381 tcatgccgga catgaacacg cccagatatg gtgccatatt cgatctggac ggcacgctgg
     1441 tggcttcgga agtcctttac cagcgcgcca ccggctttat tctgaaaaag gccgggcgat
     1501 ccctttccga actgacccca caggaaaaag ccgcgattcc gggacgttcc gcaatccgaa
     1561 acatgcggtt ttactgcgaa cggttcgaac tcccgtggag tcccgaattc ctggtcgaaa
     1621 agcgcatgga aatcgtggcc gggctggtgc gcgaagaagg cgtgatgctg atccccggcg
     1681 ccatggaatt ctgcgagatg ctgcgtgccg agccattccg catggcggtg gcatcctcgt
     1741 cgcccggccc gtatgtcagg cttgtcctgg aaacgaccgg cctggccggc cttttcgacg
     1801 ccgtgctttc cggcgacgac gtgaccgccc acaaaccgga tcccgagata ttcctgacgg
     1861 ccgccgcaag gcttgaatgc ccacccagaa gctgcctggt ttttgaggat gccgactccg
     1921 gcctccaggc ggcaaaggcg gcaggaatga agtcgatact gatcgacaat gaaacaatcc
     1981 ttccggaaca gaaacttggc gcgacccggg tgatcccgca ttattgcggc cttgggattt
     2041 ctgaagtgaa acgggtgatc aacggcggaa tcatcctgct tgattaaggc gggacgcggc
     2101 ggtgcgcact tgatggccag cggtgccagg ggggcttgaa ggcatggcaa caagggaact
     2161 tccgcgcacc ctttcgctta cgaccgcgac cgccattgtc atcggctcgg taatcggttc
     2221 cggaatcttc aaaaaatgct cttcgatgac cgacgcactc ccgtcgccaa ccctggtcct
     2281 gctggtgtgg gtactggccg gcgtcgtttc cctgatcggg gccctctgcg cggccgaact
     2341 tacggccgcc tggccagatg ccgggggcct ttacgcacac ctgcgacgcg ccctgggacc
     2401 tttctggggg ttcctttacg gctggtcaac gctttcggtg attcagaccg ggtcgattgc
     2461 ttcgatcgcc tatgtatttg ccgaatacct gcgatttttc gggggatggg gggatgtccc
     2521 gccggaactg gctgcctggg gggtcaacct tttcggggtc atcgacattt acccgctgcg
     2581 cgacctggga accaaggcgg tcgcggtgtc atgcataacc ggacttaccg tcgtcaacat
     2641 agtcggggtg cgaccaggcg cggccatcca gaacgtgttc atgtacctga aatttgccat
     2701 catgttcgga atcatcggcg tcgcggcctt tggcgcaggc cacctgctgt cggcggtttc
     2761 cggcccgatc ttcccgcagg cgaacggcct tcaggccacc tcggtggaac catccttcac
     2821 gatgctggcg ggcgggttcg ccattgccct ttcaggcgcc ttctgggcat acgacgcctg
     2881 gatctgcgcg acctacgtgg ccgccgaggt caagcgcccc gaaaggaaca tgccgattgc
     2941 actggtggcc ggcatggcgg ttgtctgcat catctacctg ctggtgaatt ccgcgtactt
     3001 ccaccttctt accgtcgatg aaatcaggaa ttccaccctt gtcgcttccg acgccctcgg
     3061 ccgggtcctt ccacttgcgg gcgcgctggt tgccgccgca gtgtgccttt cgaccttcgg
     3121 caccatcaac ggaaccgcgc tctcctcggc cagggtctat tacgccatgg ctcgcgacgg
     3181 acttttccac gaaagacttg ccgatgtcca tccacgaagg cagacgccgc acgtggccct
     3241 gatcgtccag ggcatctggt cgtcactgct ggtcttttcg gggactttcg accagataac
     3301 cgacatgctc atcttcgttt cgtggggatt ctacgcgctt cttgctgttg ccgtgcttgt
     3361 cgcgcggcgc cgttttcccg acacgccaag acccttcaag acgcccggat atccgtggct
     3421 tccgacgatc tttatcgtgt tttcagtggc ctatctcata ttttcggtcg cggccaacac
     3481 gcgcaacgct ctgatgggaa ccttgctcgt ccttccggga ataccgtttt acttccactt
     3541 caaaaaacat cggtcggcgg cggttccccg accgtgactt cggccctgcc gctcctgccg
     3601 tccatcctga tcgtcaaggg tgccggagcc cttgcgtgga tgcagtgcgc cgttctttcg
     3661 accacttccc atttcattac cgcgtcgata tcgacccagc agtcgtgcct gccccacaga
     3721 accttcatgt acccggcatt cctggcgacc aggttgtgga agaaatgcgt cccctgggaa
     3781 gattcaatcc tgaaatcctg aagatccatc tcgactatcg cgaccgcctg gctgatgtcg
     3841 gaccacttga ccggaatccc caggaatcgg tcacttgatc cccaccgacc ggggcccgcc
     3901 agcaggtact cgcggcccga ttcgcgcatc cccgcgttga ttcgggcaat ctcgtccctc
     3961 atggcgaggg tcgccgtggt gctgaatttt tccacatcga ccagcacgac gtcagtgacg
     4021 ccttcaatct ggccgtttcc caaagccccg gaagtgtgca ggatgacttc cggcggggcc
     4081 gaatcccttg tccgcccagg cacatcgaca aaaacccgat tgacggcaag gggccttatc
     4141 tgcagcacga agaactcgcg gcggccttcg gggccgggcg tgatgttcgc ggcgaactcg
     4201 acctcgaccg gaaggccgaa cgcctcttca ccaagcgcaa gaacgtcctt cacgatggcc
     4261 gcaagcggga aacttccgta tttgatgacc ggggcgaaat caagaacccg cgggcccgga
     4321 ccctgaagat ccggaaccag catgtcgccg tccatgtccc atcgcgatcc aatcaaaccc
     4381 aggactccgt gcctttcagc ctcaagaatt cccagcctgt ccatcgtcgc gacctcgtcg
     4441 ccttccatgg catcggtcgt ggcgtcaagc cgcaatgccc agaaatcctt ctgggaatcc
     4501 ttgaccagat cccctggagt caggatgtcg gtgccgggaa actgtggaca gaatctgaat
     4561 atgcgcccgc cgcttacaat cgtgcgccca agccccaggg caatcccggc aacaccgtca
     4621 gacgacttca gggagccggt gggatagaag ttatacgact gggcaacgcc tgaaatgtcg
     4681 gggtaaaagt aattcccgtg aagccttccg acaagttcct ggattatcac cgccatcttt
     4741 tcgtcgcggg gaagaaacct gcttccggca agaaaatccc tggccctgct ttcatatacg
     4801 cttgaaaaca ccaatttcac ggccttgcag agttcatgac aacgctgccc aatctcggac
     4861 gccgaattcg ggatcatcac agtgcgatac acgccggcca gcggacacgc ctgggaatcc
     4921 tcgagaagac cggaagaccg gacggccagg ggaacatcaa tcatctgtac aagttccagc
     4981 agacggcgct tgagttcatc gccaagcgac ccctgaagaa atcttgtgcg tatctgatca
     5041 tccgtcatat ccggcggatc gccatcaata ccgtttcttt cgatgaagtc gtcgtactcg
     5101 gccgtcccta tgaatgcggt caatggaagg gatacatcca caccgggata ccgcctggac
     5161 atcgccatca tgttcaggga cgagttcagg aaagccaggc cgcgaccctt gcctcccatt
     5221 gatccacccg aaaggcgcac gatttcggcg cggttggaaa cctgccagtc aattacgtca
     5281 acgaccttac cgcgattggc ggcgcgcctt acttccccga acacttcaag aaggtaccgc
     5341 ctgacatcgg cgacggaatc gaaatttgac atcccaagtg gctgtatgcg cctggcaaaa
     5401 tcgatttcac catgtgccat caaccaaccg gagtaatgat tataccttgc gtgccattcc
     5461 aggctctcgt ccgggacggt tgccagcgct tcgataaact cggaaatgtt tgaaacactt
     5521 gtgatctcgg tgccgtccgg catccggaat atgaaatcgc cgaaaccgag ctgggtaacg
     5581 atgaaatcct tcaattccat cgtgagtgac ggcgagttct tatcgacaaa atgcgcacca
     5641 aggctttctg ccttcgatcg gttttccggc gatgccgatt gaagcagaac cggcagcgtc
     5701 gcgtttcggc tgcgaaccat gcgaaggaag gcgacgccag cttcgtcatc cagaacaccg
     5761 ccgcgcatgt actgaacatc cgaaatgatc ccgatcagat
//
LOCUS       DAOPPA010000176         5769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15219_length_5769_cov_3.279314, whole genome shotgun sequence.
ACCESSION   DAOPPA010000176 DAOPPA010000000
VERSION     DAOPPA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_5769_cov_3.279314"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="PKH54_06645"
     CDS             <1..1975
                     /locus_tag="PKH54_06645"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOC99603.1"
                     /translation="DSTATDVGADTSGGDVSGGDVSDSGPVGAGWCDDPSVRIVGVNP
                     WPGGGIQVYVHGGDSESPFSMMGPTGLAVPVEAAPAPQEGGIVGILVQPSADAAIHQA
                     RLDVAAQLVESLNPSYRVAAWQVRDKAQPQLLFDLSAARSLASEKIGRIASSDVGLTA
                     FDVNFVRGLLAGAEGRWGALVRDVVVISDGSVPDGLSVGGSLDPAPKVGAVFLGDQPV
                     AGLLSDTALIRRVGACPGAAEGDSFTLTVGAATCVVTAPKPTMHLAGLECRPDQAAAD
                     LFPFGDTIELVMTPEQESVWQQRHEQKSKDDFELSVILGAGEPIPAMAHFRGQTSMDC
                     QRKNYDVNLEGGQSRRLMPGGGGDEFFLISMCKDEGFFNQVFANRLMAAWDLFPLKSR
                     LVRLRVNGANHGLYLMMQEPSDTLVKAHVALDGIVRRPFEPDPREQYEVKHSASGDIA
                     AVLQRYLDLVQVAQNVDSPAALADLRDRLDLPSYLRWMAVMTFLHNGDYVDEVFFYGS
                     FEAGGKVFWRPMNWDADDLFSQCHHGGVKALPDDWGMLYCAEGFIDQAIVESPEVYEE
                     WVDILSSVMESLDAATATEIMNGVRDDLFALLDDETAAALVEFHASFPEAVTSVGART
                     AIQGLMNQMLSAIESSRATLGGKIQAYREGVAN"
     gene            1972..4740
                     /locus_tag="PKH54_06650"
     CDS             1972..4740
                     /locus_tag="PKH54_06650"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOC99604.1"
                     /translation="MNLSIQRVVRFSAQYPLLLAVLLALSGILGCGQSSPGDVLNPDD
                     LSADVAADSVFDAPPFDLPDEPDGDDLDDGATADDGDTPQNLEIVRFSGPASVPPGSI
                     VPVVVGVDDVSFFGRMAITADGQRLQPMVVRGRGSIPFQAPAAPGQVRFAVVQAGYSG
                     EFVLNVEERSVVDVSGTLSGDQLDWNDDEDVVVSADVTVPSGSTLRIGAGTRIRLVEN
                     AQIRVEGELEVSGSASSPVLFLPDVESGGHGWGEINVKGAARIENAWFVGGGADATRA
                     FGHSDSQPVIRVAAGASVNMSGGGVTDCPGKAFASQSATVILQGLLITRVDQGGEHKA
                     SKVQVTHSHFMHIPDDDGRADDDDNDGIYLSDTEGVGPSCLVQDCVFFQGEDDAIDHN
                     GASVVIDRTWIRQFAHEGIAASKGRDVIVVDSVIMNCDQGIEAGYEGPQVFVNNCLLT
                     GNGVGLRWGDSYEWPSTGTLTVRNTVSVGNSRHNVYFNDDQQGAPPEGSATVMCSMVD
                     DAAFNGNDGNLEGMPLWNGSGCVTDSYRDRPEGCGPLGPQVCDSPPPVFSVSVTIPES
                     DWKSMHDNPQTEKEFKAGLKVGGVRFSDVIMQVHGGFARTVPKLSFRFEIPDAQAVEL
                     DLFGDGPQKQRRFVLKAGWIDGTWIREYIALDLIRRVGGLAPRVSFVELFVNEEWQGL
                     YLLVERIDKIYLKSQGLASEDVCLLKAENHQANWKQKDNPMDGYDLQQGTDDCSSAID
                     ALLRACSLTPTNAAAFDVDVWPVLSMDDFMAFEYVRIFCGDRDAFTKNYYLHFAPEGG
                     GWSKARVINWDGDAVFGNNWDGVPIAADDAAWYGRDSFVPRMIAVPEMRAAYLGRFAT
                     SLRTAFLPVILKNRVTRVAEVIRPVAIRDLLQWDRGMDFQAELDRLMGAIELRWQTMN
                     GVVSFQQR"
     gene            complement(4747..>5769)
                     /locus_tag="PKH54_06655"
     CDS             complement(4747..>5769)
                     /locus_tag="PKH54_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99605.1"
                     /translation="NLVRRTPAGFMRGPSRNPENLDDLPLPAWDLIGPEHYPDSPIGA
                     FVDALPVVPIITSRGCPCRCGFCGAHLAGTKIRLRSPEAVVDEMEYLHKRHGVREIQI
                     LDDNFNWHRSHAEGVCEQILKRDLKIFISFPNGLRLDHLDADLVRLFERTGCRSATIG
                     IESGSQKTLDRMNRRITKEKMEHLLREIKANSSIRLTGNFIIGVPGETAADIEQTIEY
                     AASLPIDRASFMVYLPLPGTDFFNELKAAGKLSSMDYGHLSPNEGVVPFVPDGMTPSD
                     IRRFLFRCYARFYGRPAILKGLLHEVHSPGQLGFLLGKTLGRLSGPRIGGPSLKALQA
                     IERGLA"
BASE COUNT         1057 a         1605 c         1874 g         1233 t
ORIGIN      
        1 cgattccacc gcgaccgacg tgggtgccga cacctcgggc ggcgacgttt cgggcggcga
       61 cgtttcggac tcggggcctg tcggcgccgg ctggtgcgac gacccttcgg taaggatcgt
      121 cggcgttaat ccgtggcccg gtggcggtat ccaggtttac gttcatggcg gtgattccga
      181 atccccgttt tcaatgatgg gcccaaccgg cttggcggtg ccggtcgaag cagcccccgc
      241 gccgcaggaa ggcggcatcg tcggaatcct tgtacagccg tcggccgatg cggcgatcca
      301 ccaggccagg ctcgacgtcg ctgcgcaact tgtcgaatcc ttgaatccgt cttatcgggt
      361 cgccgcatgg caggtgcgcg acaaggccca gccgcagctt ctgttcgatc tgtccgcggc
      421 caggtcgctg gccagtgaaa agatcgggcg aattgcatcg tccgatgtcg gcctgaccgc
      481 cttcgacgtc aacttcgtac gcggtctgct tgccggggcg gaaggacgct ggggagccct
      541 tgtaagggat gttgtcgtga tttcggatgg ttctgttcca gatgggcttt cggtcggcgg
      601 cagtcttgac ccagccccga aggtcggtgc cgtatttctg ggcgatcagc cggttgccgg
      661 cctgttgagc gacaccgcat tgatccggcg tgtcggggcc tgtccgggcg ctgcggaagg
      721 cgactcgttt actctgaccg tcggcgcggc aacttgtgtc gttacggcgc cgaaaccgac
      781 gatgcatctg gccggtctcg agtgccgtcc cgaccaggcg gcggccgatt tgttcccgtt
      841 cggcgacacg attgaactgg tcatgacgcc ggaacaggag tcggtgtggc agcagcgcca
      901 cgaacagaag agcaaggatg acttcgaatt gtccgtgata ctgggcgcgg gcgaaccgat
      961 tccggccatg gcgcacttcc gtggccagac gtccatggac tgccagcgca agaattacga
     1021 tgtcaatctt gaaggcggcc agtcaaggcg ccttatgcca ggtggtggcg gcgacgaatt
     1081 ctttttgatc agcatgtgca aggatgaggg ctttttcaac caggtgttcg ccaaccgctt
     1141 gatggccgcg tgggatctgt tccccttgaa gtcgcgcctg gtcaggcttc gtgtaaacgg
     1201 cgccaaccac gggctttacc tgatgatgca ggaaccgtca gacacgctgg tgaaggcgca
     1261 cgtggcgctt gacggcatcg tgcggcgacc gttcgagccc gatccgcgcg aacagtatga
     1321 agtaaagcat tccgcatctg gtgacatcgc ggcggttctg cagcgatacc ttgacctggt
     1381 gcaggtcgcc cagaacgttg acagcccggc tgccctggcc gaccttcgtg acaggcttga
     1441 tctgccgtcg taccttcggt ggatggcggt gatgaccttc ctgcacaacg gcgactacgt
     1501 tgacgaagta ttcttttatg gaagtttcga ggcgggcggc aaggtgttct ggcgcccgat
     1561 gaactgggat gccgacgacc tgttcagcca gtgtcaccac ggcggcgtca aggcgctgcc
     1621 cgatgactgg ggcatgttgt attgcgcgga aggtttcatt gaccaggcca ttgtcgaatc
     1681 gccggaagtc tatgaagaat gggttgacat cctttcgtcg gtgatggaat cgcttgatgc
     1741 ggcgaccgcg accgaaatca tgaacggcgt cagggacgac ctttttgcgc tgcttgacga
     1801 cgagaccgcg gccgcactgg ttgaattcca cgcatcgttc ccggaagcgg tgacgtcggt
     1861 gggggccagg accgccatcc agggattgat gaaccagatg ctttcggcga ttgaatcaag
     1921 ccgggcgacg ctggggggaa aaattcaggc atacagggag ggcgttgcca attgaacctg
     1981 tcgattcaac gtgttgtcag gttttcggca cagtatccgc tgttgcttgc ggtgctcctg
     2041 gcgctttccg ggatcctggg ctgcggccag tcgtcgcccg gtgacgtctt gaatccggat
     2101 gatttatccg ccgacgtcgc ggccgattcg gtgtttgacg cgccgccttt cgacttgccg
     2161 gatgaacctg acggcgacga ccttgacgac ggtgcgacag ccgatgacgg cgatacgccc
     2221 cagaaccttg agatagtgcg tttttccggc cctgcaagcg taccgccggg atcaatcgtt
     2281 ccggtcgtcg tcggtgtcga tgatgtttcc ttttttggcc gcatggctat cacggcggat
     2341 gggcagcggc ttcaaccgat ggtcgttcgc ggtcgcggat cgattccttt ccaggccccc
     2401 gcagcacccg ggcaggtgcg gttcgctgtc gtccaggccg ggtattccgg ggagttcgtc
     2461 ctgaatgtcg aggagcggtc ggtggtcgat gtctcgggga ccctttccgg tgatcaactt
     2521 gactggaatg atgatgaaga tgtcgtggtc agtgccgacg tcacggtgcc ttcaggctcg
     2581 acccttcgca tcggggccgg cacaaggatt cgcctggtcg aaaatgccca gatccgggtc
     2641 gagggtgagt tggaagtttc cggttcggcg tccagcccgg ttctgtttct tcccgatgtg
     2701 gagtcgggcg gtcacggatg gggcgagatt aacgtgaagg gtgccgcccg aatcgaaaat
     2761 gcgtggttcg tcggcggtgg tgccgatgca acccgggcct tcgggcattc ggattcccag
     2821 ccggtcatcc gcgtcgccgc cggagcgtcg gtcaacatgt ccggcggtgg cgtcaccgat
     2881 tgtccaggca aggcgttcgc atcgcagagc gcgacggtga tccttcaggg cctgctgatt
     2941 acgcgcgttg atcagggcgg ggaacacaag gcatcgaagg ttcaggttac gcactctcat
     3001 ttcatgcaca ttcccgatga tgacggccgc gccgacgacg atgacaacga cggcatctat
     3061 ctgagtgata ccgaaggggt cggcccttcg tgcctggttc aggattgcgt tttctttcag
     3121 ggcgaggacg acgcgattga ccacaatggg gcatcggtcg tgatcgatcg cacgtggatc
     3181 aggcagttcg cgcacgaagg gatcgcggcg tccaaggggc gcgatgtgat cgttgtcgat
     3241 tcggtgatca tgaactgcga ccagggcatc gaggccggat acgaggggcc acaggtattc
     3301 gttaacaact gtctcctgac cgggaacggc gtcggcttgc gatggggcga ttcatacgaa
     3361 tggccatcga ccggaacgct gaccgtccgt aacacggttt cagtcggcaa ttcacgtcac
     3421 aatgtgtatt tcaacgatga ccagcagggc gccccgcccg aaggctcggc gaccgtcatg
     3481 tgcagcatgg tcgatgacgc cgcgttcaat ggcaatgacg gcaatcttga agggatgccc
     3541 ttgtggaacg ggtcgggatg cgtgacggat tcctatcgcg accgtccgga aggttgcggg
     3601 ccgctgggac cccaggtgtg cgacagtccg ccgccggtct tttcggtttc ggtcacgatc
     3661 cctgagtcgg actggaagtc gatgcacgac aatccccaga ccgaaaagga attcaaggcc
     3721 ggcctgaagg ttggtggagt tcggttttcc gacgtaataa tgcaggttca tggcggcttt
     3781 gccaggacgg ttccgaaact ttcatttcgc ttcgagattc cggatgcaca ggccgtggaa
     3841 ctggatctct tcggggacgg gccgcagaag cagcgccgtt tcgtcctgaa ggccgggtgg
     3901 atcgatggta cctggattcg cgagtacatt gcattggatt tgatcaggcg cgtcggcggt
     3961 ctggcgccgc gggtttcgtt cgtggaactg ttcgtcaatg aggaatggca gggcctttac
     4021 ctgctggtcg agcgtatcga caagatatat ctgaaaagtc aggggcttgc atcagaggat
     4081 gtctgcctgc tgaaggccga gaatcatcag gcgaactgga agcagaaaga caatccgatg
     4141 gacgggtatg accttcaaca ggggactgac gactgttcgt cggcaatcga tgccctgctt
     4201 cgcgcatgct cgcttacccc gaccaacgcg gccgcatttg atgtcgatgt ttggcccgtg
     4261 ctgagcatgg atgacttcat ggcgtttgag tacgtccgca tcttctgcgg tgatcgcgac
     4321 gcctttacca agaactacta tctgcacttc gcgccggaag gcggcggctg gtcgaaggcc
     4381 cgcgtgatca actgggacgg cgacgccgtt ttcggcaaca actgggacgg cgtgcccatc
     4441 gccgcggacg acgcggcctg gtatggaagg gactcgttcg tgccaaggat gattgcggtt
     4501 cccgaaatgc gcgcggctta tctgggacgg tttgccacat cactgcgcac cgcctttctt
     4561 ccggtcatcc tgaagaaccg cgtgacccgc gtggccgaag tcatccgtcc cgtggcaata
     4621 agggatttgc ttcagtggga tcgcggcatg gactttcagg cagagcttga tcgcctgatg
     4681 ggcgcgattg agcttcgctg gcagacgatg aatggcgtcg taagtttcca gcagcgttga
     4741 tgcaggtcac gcaagtccgc gctcgattgc ctggagagcc ttcagcgacg gcccgccgat
     4801 gcgcggcccg gacaggcgcc ccagagtttt tccaagcagg aatccgagct ggcccgggct
     4861 gtgaacttca tgaagaagtc ccttcaggat ggccggcctg ccatagaacc tggcgtagca
     4921 acggaaaagg aagcgcctga tgtcggatgg ggtcatgcca tcgggaacga acggcacaac
     4981 gccttcgttc ggggaaagat gaccgtaatc catcgacgag agtttcccgg cggccttcag
     5041 ttcattgaag aagtcggttc ccggaagcgg aagatagacc atgaaggaag ccctgtcgat
     5101 cggcagcgat gccgcatact cgatggtctg ctcgatatcc gcggccgttt cgcccgggac
     5161 acctatgatg aagttaccag tcagccggat actcgagttt gccttgattt cgcgaaggag
     5221 gtgttccatt ttttccttcg tgatccggcg gttcatgcgg tcaagcgttt tctggcttcc
     5281 cgattcgatt ccgattgtcg ccgatctgca accggtgcgt tcgaaaaggc gcaccagatc
     5341 cgcatccagg tgatccaggc gcaatccgtt cgggaaggaa atgaaaatct tcaggtcgcg
     5401 cttcagtatc tgttcgcaga cgccttcggc gtggcttcgg tgccagttga agttgtcgtc
     5461 cagaatctgg atttccctga cgccatggcg tttgtgcagg tactccattt cgtcaaccac
     5521 ggcttccggc gaccgaagac gtatcttcgt cccggccagg tgggcgccgc agaaaccgca
     5581 gcggcaggga catccgcggc tggtgatgat cgggacgacc ggaagggcat cgacgaaagc
     5641 cccgatcggg ctgtccggat aatgttccgg accgatcaaa tcccaggccg gcaacggcag
     5701 atcgtcaagg ttttccgggt ttcgcgacgg tccgcgcatg aaaccggccg gcgtccgccg
     5761 gacaagatt
//
LOCUS       DAOPPA010000177         5645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15692_length_5645_cov_3.338998, whole genome shotgun sequence.
ACCESSION   DAOPPA010000177 DAOPPA010000000
VERSION     DAOPPA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15692_length_5645_cov_3.338998"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKH54_06660"
     CDS             <1..397
                     /locus_tag="PKH54_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99606.1"
                     /translation="VDENIRQLCAINYYPKDYKYLEYIVCRNKDIRSSDWKRCAMNGI
                     DPDVIRRCADGSEGRRLLSKNLKFARQLEIGASPTWLANNKFKFSGIAPEQIRKQFCD
                     HNSNLSGCRKTLSGEAIGGGTGAPTSGSN"
     gene            399..4145
                     /locus_tag="PKH54_06665"
     CDS             399..4145
                     /locus_tag="PKH54_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC99607.1"
                     /translation="MNRSRSASLATVLMLLSLLLQGCAGLPTVLRELIDQGANEKAQA
                     RGEKWLEGHAGKPKYKEEADVITVLVAEATLNLAIKADTVEAYRAFKGKYSRVKGCDE
                     FLKTALLNEATANYRDFVTKRNDINAYQAFRKEYPGAREFVDSVRREAELALADSLKV
                     PGIEAVRKFLAQYDGRDGARDSLKKAREALVNRAFEGAVGSMDYQKIKAFRTEFESWP
                     EVAPYIARARETECRRGFERAEKSQNVNLISMFRSQYESWPEAAELVARAYDLEAGIA
                     LSEAFRARDVARLQQLGSIYGKGTWPEKFGAAVSDVYLERLEKAINVGKAFPEDFVNR
                     FLEDVRSVPGIQARGERLRAELFKTARALKDPPALYLAGTFFSDDPESPKLLTQAEAG
                     YWKVVNEVDQMDRWLQYTRWFPASPRAATAEQRYLWHQEIRNREAFGFIGNITRTIRQ
                     PNGDIDVYVDVRDKKGNRVSGLTRDAFRLFLGSLNVEIIDFWGMEDDRPLDIMFAIDM
                     SGSMSPEKQAVRDAVAHFASVLDFRGRDARLGIVSFSDEVIDKQGFSSDSNKFIGWMN
                     SLMSTTVGGAGEDGVQAMMDSIGMLRSSPGERVVIFLSDEPLQVNSGGLERLRMEPGD
                     DCGRARAWVACRNRCQENERKGLVGPTTGAIGQTVFIGGPQCEASCVSKLGREASRLF
                     SRCMGRADVSSCVRDSSLMSMAMGMNRGCGDPVITQGSRETAALIREMDRAQLRPYFI
                     VTPRDSAPGTGYAAIAESLQGRVLAVPDDTRSSEPYVRVLMEIAEQLSRQYVVRYRPN
                     KSIRKDASATVVVRPIHRWSAFGNVPQADVMAMVPTSDSIGCPDFLVFTRTGLFASSG
                     CSANWEDRHIGLSSRIIDARVGSEHVLLLTQDQKIWLYGIADGSLDEVPLGFKTIADF
                     QWEGSDGFWVSGLVPDGSILVEFRQVGASDTVKSYAIAAAERPGRVSGTVFPVVMSVG
                     RDSKDVCMLVKPDLAMCSADGRTDWKQFKVQGLPSNALSGPARLSRISAHQDSMILVA
                     ADGSIFRSISNGSLWTRVLDPVPGGRQLSQYFTKPELLCATSSQDVVCSEDGGANFFP
                     LGLKYKPGQGFAMASVGGHPVLASEGSIQRLFRVLNRELPSSAVYFDTNDSSPSASMM
                     PFLRDIANEMRRNPDSHLRIEGHADARGTTQHNEELSRLRAEAIASEMTSMGVERDRL
                     MVVWFGERRPIQAGESAKAHARNRRVELILLKTLPDRWFEAGGR"
     gene            4174..>5645
                     /locus_tag="PKH54_06670"
     CDS             4174..>5645
                     /locus_tag="PKH54_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99608.1"
                     /translation="MKKCLTCNLTYEDSSRFCSRCGGGLVMEQVAGGVPFQTGSGRDM
                     AYAQQGSSGAAVLIAQALQLQVKGSVGQACRLLEKGAEAYPGDIELRQRLVALLVQSR
                     KFLQAIRHLQVLVTHCPDMMSAWLLLVEALIASGREDSVPDLLRRAAAIDCRDNQIVR
                     LKDLLMRQKMYPEALSVSKRIVSIWPEDISAWKDLAICSWRLGESDVELDAWQRVLKV
                     TPGDAMASLRAGLICAVTGKASPQECVNLLERAVADEHGGLDEIDVIQARAVLAGFLA
                     AGGRVNQAIGVLKSIAPPDAGEHPELLQLLARKWLEAANVLRAEGQWESAHEAYGNAR
                     DLGLRNDAREGLVETCLALAANASGVGMTAAAAAYACEAADSSDDPELHARAAGLCMD
                     CADELEARGDHQGALRFLRIAGSLSGSRDEAVRGRLRSVESALATGRIRKLAVFFAAL
                     VLVASGAGAFLLAKGDVQIKVKDAVRVSLLDDGVQIKSVEG"
BASE COUNT         1131 a         1503 c         1823 g         1188 t
ORIGIN      
        1 agtcgatgaa aatataagac aattgtgcgc gatcaattat tacccgaagg attacaagta
       61 cctggaatat atcgtgtgcc gcaacaagga tatcaggtcg tccgattgga agcggtgtgc
      121 catgaacggt atcgacccgg atgttatccg gcgttgcgcc gacggatccg aagggcgcag
      181 gcttctgtca aagaatctga agtttgccag acaactggaa atcggcgcca gtccgacctg
      241 gcttgccaac aacaagttca agttctcggg catcgccccg gaacagatca ggaagcagtt
      301 ctgtgatcac aattcgaatc tttcgggatg caggaagacg ttgtcaggcg aggccattgg
      361 tggcggaacc ggcgccccga catccgggag taactgacat gaatcgttcg cgcagtgcgt
      421 cacttgcaac cgtactcatg ctgctctcgc ttctgttgca gggctgcgcg gggcttccga
      481 ccgtgcttcg cgagcttatc gaccagggtg cgaacgaaaa ggcccaggcc aggggcgaaa
      541 agtggcttga aggtcatgcc ggcaaaccga agtacaagga agaggccgac gtgattacag
      601 tccttgtcgc cgaggccacc cttaacctgg caatcaaggc ggacaccgtc gaggcgtacc
      661 gcgcgttcaa gggcaagtac tcgcgtgtca aagggtgcga cgaatttctg aagaccgcgc
      721 ttctgaatga agccaccgcc aactatcggg acttcgtgac gaagcgtaac gatatcaacg
      781 cgtatcaagc cttccggaag gaatatccag gcgccaggga gttcgtcgat tctgtccggc
      841 gggaggccga actggcactc gccgactcac tgaaggttcc cggaattgaa gcggtcagga
      901 agttccttgc gcagtacgac ggccgcgacg gagcccggga cagtctgaag aaggcaaggg
      961 aagccctggt caatcgagcc ttcgaaggtg ccgtgggatc catggattac cagaagatca
     1021 aggcatttcg aaccgaattc gaatcttggc cggaagtcgc tccatacatc gccagggcaa
     1081 gggaaaccga gtgccggcgt ggttttgaac gggccgagaa aagccagaac gtcaatctga
     1141 tttccatgtt tcgaagtcag tacgagtcat ggccggaagc ggccgagctt gtcgcccggg
     1201 cgtacgatct cgaggctggc atcgcgctgt ccgaggcgtt tcgggcgcgc gatgtggcac
     1261 gtctgcagca actgggctcc atatatggaa aggggacctg gccggaaaag ttcggggccg
     1321 cggtttcgga cgtctatctg gaaaggctcg agaaggcgat aaacgttggc aaggcatttc
     1381 ccgaggactt cgttaaccgg tttcttgaag acgtccgctc ggtgcccgga atccaggcgc
     1441 gcggggaaag gcttcgggca gaactgttca agacagccag ggcgctgaag gatcccccgg
     1501 cgctttacct ggcgggaacg tttttttccg atgatcccga gtccccgaag ctgctgaccc
     1561 aggctgaagc cggttactgg aaggtggtga acgaagtcga tcagatggat cgctggcttc
     1621 agtacacaag atggttcccg gcaagtccaa gggcggcgac ggccgagcag cgatatctgt
     1681 ggcaccagga aatccgcaat cgcgaggcgt tcggtttcat cggaaatatc accagaacca
     1741 tccgccagcc gaacggtgac atcgacgtct atgtcgatgt ccgcgacaag aagggcaacc
     1801 gagtgtcggg cctgacccgt gatgccttcc ggctgttcct tggatccctg aacgtcgaga
     1861 tcatcgattt ctggggaatg gaagatgaca ggccgctgga catcatgttt gcaatcgaca
     1921 tgtcgggcag catgtccccg gaaaaacagg cggttcggga cgccgtcgcg catttcgcca
     1981 gcgtgctgga tttccgcggc cgcgatgcgc ggcttggaat cgtgtccttt tcggacgagg
     2041 tcatcgacaa gcagggattt tcatcggatt cgaacaagtt catcggctgg atgaattccc
     2101 tgatgtcaac cacggtcggt ggtgccggcg aggacggcgt tcaggcgatg atggattcga
     2161 ttggcatgtt gcgttcgtcg ccaggcgaaa gggtcgtgat attcctttcc gatgaacctc
     2221 ttcaggtcaa ttccggcggc ctcgagcgcc tgaggatgga gcccggcgac gactgcggcc
     2281 gtgccagggc atgggtggcc tgtcgcaacc ggtgccagga aaatgaacgc aaggggcttg
     2341 tcgggccgac cactggcgcc atcgggcaga ccgtcttcat cggcgggcca cagtgcgagg
     2401 cgtcctgcgt ttccaagctt ggtcgggaag cctcaaggct cttcagccgg tgcatggggc
     2461 gtgccgatgt ttcttcatgc gtcagggaca gttcgcttat gtccatggca atgggcatga
     2521 atcgtggttg cggggatccg gtaattaccc agggcagccg cgaaaccgcc gcgcttatca
     2581 gggaaatgga tcgtgcccag ttgcggccgt acttcatcgt caccccgcgt gattcagccc
     2641 ctggaacagg gtatgccgcc attgctgaaa gccttcaggg ccgggttctt gcggttcccg
     2701 acgataccag gtcttccgaa ccttatgtca gggttctgat ggaaatcgcg gaacagcttt
     2761 cgcgtcagta cgttgttcgc tatcgtccga acaagtcgat caggaaggat gcgtcggcaa
     2821 ccgtcgttgt caggccgatt catcgctgga gtgcttttgg aaacgttccg caggcggacg
     2881 tcatggccat ggttccgacc agcgacagca tcggatgtcc cgatttcctg gtattcacgc
     2941 gaaccggtct tttcgcatct tccggctgtt cggcgaactg ggaagatcgc cacatcggcc
     3001 tatcgtcccg gattattgat gccagggtcg ggtcggagca tgtgcttctg cttactcagg
     3061 atcagaagat ctggctttac ggcatcgcgg acggctcgct ggatgaagtg ccacttggct
     3121 tcaagacgat cgccgatttc cagtgggagg gatccgacgg attctgggtg tccggactcg
     3181 tgcccgacgg ctcgatactt gtcgaattca ggcaagtcgg tgcgtccgat accgtgaagt
     3241 cgtatgcaat cgccgcggcg gaaaggccgg gtcgcgtatc cggaaccgtg ttccccgtgg
     3301 tcatgtcggt cggtcgggac tcaaaggacg tgtgcatgct ggtcaagccg gatctcgcga
     3361 tgtgttcggc cgacggccgt accgactgga agcagttcaa ggtccagggg cttccgtcaa
     3421 acgctctttc cggtcccgcg cgtctgtcgc gtatctcggc tcatcaggac tcgatgatac
     3481 ttgttgcggc cgatggctcc atattccgca gcatttcgaa cggcagcctt tggaccaggg
     3541 ttctcgaccc ggtgcccggc gggcgccagt tgtcgcagta cttcacgaag ccggaacttc
     3601 tttgcgcgac atcttcccag gatgtggtgt gttcggaaga tggcggagca aacttcttcc
     3661 cgcttggact gaaatacaag cctggccagg gattcgcgat ggcatcggtc ggggggcatc
     3721 cggttcttgc gtcggaaggg tcgatccagc gccttttccg cgtcctgaat cgcgaattgc
     3781 caagcagcgc ggtgtatttc gatacgaacg acagttcgcc ttcggcatcg atgatgccgt
     3841 tccttcgtga tatcgcgaat gaaatgagaa ggaatccgga cagtcatctt cggattgaag
     3901 gtcacgcgga tgcgcgcggc acgacccagc acaacgagga attgtccagg cttcgggccg
     3961 aggcaattgc cagcgagatg acatccatgg gggttgaacg ggaccgcctg atggtggtct
     4021 ggttcggtga aaggcgaccg atccaggctg gtgagagcgc gaaggcgcac gccaggaacc
     4081 ggcgcgttga gctgattctt ttgaagacct tgccggacag gtggtttgag gcgggcggca
     4141 ggtgaaggcc atcattcagg acggagatgc agaatgaaga aatgtttgac ctgtaacctg
     4201 acttacgagg actcaagtcg tttctgttcc aggtgcggcg gcggtctcgt catggagcag
     4261 gtggccggcg gcgtgccatt ccagaccgga agcggacggg atatggcata cgctcaacag
     4321 gggtcgtccg gggccgccgt cctgatagcc caggcgcttc aacttcaggt gaagggttcc
     4381 gttggccagg cgtgccgctt gcttgaaaag ggtgccgagg catatcctgg tgacattgaa
     4441 ttgcggcaga gactggttgc actgctggtt caatcccgca agtttctgca ggccattcgc
     4501 catcttcagg ttcttgtcac ccactgcccg gatatgatgt ccgcatggtt gcttctggtc
     4561 gaggcgctga ttgcatccgg gcgcgaggat tcggtgccgg atttgcttcg gcgggcggcc
     4621 gccattgatt gtcgcgacaa ccagatagtt cggctgaagg atctgttgat gcgccagaag
     4681 atgtatccgg aggcgctttc cgtttccaaa cggatagttt caatctggcc tgaagatatt
     4741 tcggcctgga aggatctggc aatctgctcc tggcggcttg gggaatccga tgttgaactg
     4801 gatgcgtggc aaagagtcct gaaagtcact cctggcgacg cgatggcatc gttgcgggcc
     4861 ggcctgatct gcgccgtgac cggcaaggcg agtccacagg aatgcgtcaa cctgctcgag
     4921 agggcggtcg ccgatgaaca tgggggactt gacgaaatcg atgtaatcca ggccagggcg
     4981 gtgctggccg gttttcttgc cgccggcggg cgcgtgaatc aggcgatcgg tgttctgaaa
     5041 tcaatcgcgc cgcccgatgc tggagagcat ccggaactgc ttcagttgct tgcccgtaag
     5101 tggctggagg cggcaaatgt actcagggca gaagggcagt gggaatcggc ccatgaggcg
     5161 tacggaaatg ccagggatct tggtttgagg aacgatgcca gggaaggact cgttgaaact
     5221 tgccttgcgc ttgccgcgaa tgcgtccggc gtcggcatga cggcggcggc cgcagcttat
     5281 gcatgtgagg cagcggattc atcggatgat cccgaacttc atgcccgcgc ggcaggactg
     5341 tgcatggatt gcgccgacga actcgaggcc cggggcgatc accagggcgc cttgagattt
     5401 ctgcgaatcg ccggttcgct gtccggatcc cgcgatgaag ccgttcgtgg caggttacgc
     5461 tcggttgaat ccgctttggc gaccgggcgg atccgcaaat tggcggtttt ctttgccgcc
     5521 cttgtcctgg tcgcgtccgg ggccggagcc ttcctgctgg cgaagggtga cgtgcagatc
     5581 aaggtcaagg atgccgtgcg ggtcagcctg cttgacgacg gtgtccagat caagtcggtt
     5641 gaagg
//
LOCUS       DAOPPA010000178         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15717_length_5640_cov_3.856938, whole genome shotgun sequence.
ACCESSION   DAOPPA010000178 DAOPPA010000000
VERSION     DAOPPA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15717_length_5640_cov_3.856938"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PKH54_06675"
     CDS             <1..1278
                     /locus_tag="PKH54_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC99609.1"
                     /translation="EFRIPYMVGVYLAANAIPDLYLLTDGPDCLFFKAEFVHGAQDLH
                     STLLDVTGRHRVAHTLADINNVVLDREEQISALAARLAAEPDAGMVLVTAMPMASITG
                     TQYDRIARDLSARTGKLIAEVPARSLQGDWLDGYGQVLAAVARTLPLEGAGSGNPESV
                     AVVGPLVDRTEADRLADVREIRRILQDGLGLDVCSIWPSNSTVAELGEVARAGTVISL
                     PYGARAARILARRLGARLVHAALPFGPAGTADFVRTVATALGRLDRAEDFLKAESLEW
                     GDSFKRAAASLESMKFIVAADPWLAEGLVRMLQDAGVSIAAAQSTSFDPKPVCGLPTL
                     AEVEDGVDVDMLIVPTRGIDVALRLGRPFYEFGFTSYGTHAFFDAPTLGWPGAVNVMS
                     GIVSAGTLFSTIAGSRTWNLKRLAGSYQVVPDH"
     gene            1351..3084
                     /locus_tag="PKH54_06680"
     CDS             1351..3084
                     /locus_tag="PKH54_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOC99610.1"
                     /translation="MDGGPPKITVGPEAVPAITDAFDLQRFAAAGCVLSSVEPGSDSI
                     RLLFDWVGVGSVVVSVAPDDGRPSYASTGNLSFCLIGADVSRPWAVLIARMAASAGSM
                     KMADVARLAGSGLGPADQSASPAKPAEFNPILFWAPEEGWRRFMCDAAMERRFREAFE
                     FDSDSSFIVHGDLECRFVSPGARVRLPGHFVYPFDLFSHHEAVGPITGLDDNDVIMDS
                     TRKIDLKVRRELLDGTFRGPVVVISTCVPVIVAEDPTNVLRGLDEVCPEGISYVTPVS
                     PHSPSEIFMQSFAGLRGRILSRPGRPETVGLVGYRPGRALSELQELLELAGLSVSGAI
                     VPLVTRRLLQDTLCAETIVIAPSRLHDAMYDGLVEGAAESGRRVIRPAFPWGIDATVR
                     WLEAICADCGIEVPEAAIARAEWARRLTAPGQVGKSFIFVCDPSEASRLVDPVSPAGL
                     PIVPMMCGLGLSVRLLVHGDDGDAGLALETVRNGIPAGEDLVQAASFVSRQDLDGLLR
                     EDDVAAVYSEMFFERRATRAGVPAFSPRIFEPGIDGALRTLRRFQRLASLPFYRRYMA
                     GSRFGPAGRAL"
     gene            3137..3919
                     /locus_tag="PKH54_06685"
     CDS             3137..3919
                     /locus_tag="PKH54_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99611.1"
                     /translation="MRITLVIVCAAMFVFLSSVVGCSRTHPVDPEMVILDGKPISTML
                     PTAPPPPEDPEARRRELRKHIADDMRIRSLANPVGRGELRRLSGFRYVFREAAPGTVK
                     SIPELQAVIGELQGALEQAKVEHGRPFLFIPESASGWLFNEVVRVCLPLPADAQAPSG
                     MPVDELKTTMVVFQPRRTLPPEGKLDEMERWAEKALEAAAGGPVDFSGPFIIRTALPD
                     GLLSDPEGVDWMAPSAVAVPVPGRTAEGGEPAEPAAEEDKVE"
     gene            3912..4928
                     /locus_tag="PKH54_06690"
     CDS             3912..4928
                     /locus_tag="PKH54_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOC99612.1"
                     /translation="MSEQSSSRLVDLGMRLAVPYNHDPRLIQMLQTMAASIAEIFIPA
                     PLDVSGTFRAWNGPDPVEYRKMVPGIVCAASRIGLSVNMAVNSPFVPPSEHRMVADYI
                     ANAASEGVRSFTIGDLHLAEHVRAVCPEVELVASATADIAHVTRARHWVRMAGVSRIV
                     PSRVVNKDPDVLRAMARLGVQIEIIPNEQCLPACPYMFQHAMTIGFRDSVDDTDFRRY
                     CAMCAAMKPAAPWEHYQTEIVPASIPRYRGIVSLVKLAGRDSTTSFILGEVGRFIALE
                     SDWTGFLGGYREPPDVFEKLQSCDRVCEDCGYCQSAFIEANPGWQDRGSEWLRRVTGV
                     ESGR"
     gene            5220..>5640
                     /locus_tag="PKH54_06695"
     CDS             5220..>5640
                     /locus_tag="PKH54_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOC99613.1"
                     /translation="MKLKDIGTLLELPDSNGSDIPTLAFVGQPNCGKSTLFNALAGFK
                     ANTGNFTGTSVEFTESLAVVGNRRVRLFDLPGTYSLNPSDEAEAVTRRWLDERRADAI
                     ILVLDASVLSRSLELALEMAETDMPFAIALNMMDDAAR"
BASE COUNT          935 a         1722 c         1822 g         1161 t
ORIGIN      
        1 gaattcagga ttccctacat ggtcggggtt tatctggccg cgaacgccat tccggatctg
       61 taccttctga cggatggtcc ggactgcctc ttcttcaagg ccgaattcgt gcacggcgcc
      121 caggatcttc attcgaccct gcttgacgtg acggggcgcc atcgcgtggc ccacacgctg
      181 gccgatatca acaacgtggt tcttgaccgc gaggagcaga tttcggccct ggccgcccga
      241 ctggcggccg aaccggacgc cgggatggtt cttgtcacgg ccatgccgat ggccagcatc
      301 accgggacgc agtacgaccg gatcgcccgc gacctgtccg ccagaacagg caagctgatt
      361 gccgaggtgc cggcccgttc gcttcagggt gactggctgg acggatacgg acaggttctt
      421 gccgccgtcg ccaggacgct tccgcttgag ggcgccgggt cggggaatcc cgaatcggtc
      481 gccgtcgtcg ggccccttgt tgacagaacc gaagccgatc ggctggccga tgtgcgggaa
      541 atccggcgca ttctacagga cgggctcggg cttgacgtct gctcgatctg gccgtcgaac
      601 agcaccgttg cggagcttgg cgaggtcgcg cgggccggaa ccgtgatttc cctgccatac
      661 ggcgcccgcg ccgccaggat tctggcgcgc aggctcgggg caaggctggt tcacgccgcg
      721 cttccgttcg gaccggcagg cacggccgac tttgtccgca ccgtggcgac ggcgcttgga
      781 cgcctggatc gggccgagga tttccttaag gccgaaagcc ttgaatgggg cgattcgttc
      841 aaaagggcag cagcctcgct tgagtcgatg aagttcatcg tggccgcgga tccctggctg
      901 gcggaaggcc tggtcaggat gctgcaggat gccggggtga gcatcgcggc ggcccaatcg
      961 acctcgttcg atccgaagcc ggtttgcggg ctgccgacgc tggcggaagt cgaggacggc
     1021 gttgatgtcg atatgctgat cgtcccgacc aggggcatcg acgtggcact gcggctcggc
     1081 aggccgtttt atgagttcgg tttcaccagc tacggcacgc acgccttttt cgatgcaccg
     1141 acgctgggct ggcccggcgc ggtcaacgtc atgtcgggaa tcgtatcggc gggaaccctg
     1201 ttttccacga tagccggctc acggacgtgg aacctgaagc ggcttgccgg cagctatcag
     1261 gtcgttcccg accattgatc ggcgccgggg ccaggatcaa cagggtgggc tgaccgggcg
     1321 tcaatctgcg gacggcccgg gggattatca atggacggtg ggccgccgaa aatcactgtc
     1381 gggcccgagg cggttccggc aatcaccgat gcattcgatc tgcagaggtt tgccgcggca
     1441 ggctgcgtcc tgtcttcggt cgagcctggc tccgattcca tccgtcttct tttcgactgg
     1501 gtcggggtcg ggagcgtggt tgtttcggtt gcccccgatg acggccgtcc atcgtatgcc
     1561 tcgaccggta acctgtcatt ctgcctgatc ggggccgatg tctccaggcc gtgggccgtt
     1621 cttatcgccc gcatggccgc cagcgccggt tcgatgaaga tggccgacgt ggcgcgcctt
     1681 gccggttcgg gacttgggcc ggccgatcag tcggcgtccc ccgcgaagcc tgcggaattc
     1741 aatccgatac tgttctgggc ccccgaggag gggtggcgca ggttcatgtg cgatgccgcc
     1801 atggagaggc gttttcgcga ggcgtttgaa ttcgattccg attcgtcctt catcgtccac
     1861 ggagatcttg aatgcaggtt cgtgtcgccg ggggcccgcg tcagacttcc gggccatttc
     1921 gtctatccat tcgacctgtt ttcccatcac gaggcggtcg gcccgataac cggcctcgac
     1981 gacaacgacg tcatcatgga ttccacaagg aagattgacc tgaaggttcg tcgcgagctg
     2041 cttgacggca cgttccgcgg gccggttgtc gtcatttcga cctgcgtgcc ggtcatcgtg
     2101 gccgaggatc cgaccaacgt gctccggggg ctggacgaag tgtgtcccga aggcatcagt
     2161 tatgtgactc cggtttcgcc ccacagcccc agcgagattt tcatgcaatc cttcgccggt
     2221 cttcgcggcc ggatcctgtc gcggccgggg cgtccggaaa cggtcgggct tgtcggctac
     2281 aggccgggac gggccctttc ggagcttcag gaactgctgg aacttgccgg gctgtccgtc
     2341 agcggcgcga tagtccccct tgtcacaagg cggttgctgc aggacaccct ttgcgcggaa
     2401 accatcgtca tcgcgccgtc gcgccttcac gatgccatgt atgacgggct tgtcgaaggc
     2461 gcggccgaat cgggtcgtcg agtcatccgg cccgcctttc cgtggggtat cgacgcgacc
     2521 gttcgatggc ttgaagcgat ctgcgcggat tgcggaattg aagttcccga agcggcgatc
     2581 gcaagggcgg aatgggcccg tcggctgacc gcccccggac aggtcgggaa gtccttcatc
     2641 ttcgtctgcg acccttcgga agcgtccagg ctggtcgatc cggtcagccc ggccgggctg
     2701 ccgattgtcc ccatgatgtg cgggcttggc ctttctgtca ggttgcttgt tcatggcgat
     2761 gatggtgatg cagggctcgc gcttgagacc gtccgcaatg ggattccggc cggggaggac
     2821 ctggtgcagg ccgcgtcttt cgtcagcagg caggatctgg acgggctgct gcgggaagac
     2881 gacgtggccg ccgtatattc cgagatgttt ttcgagcgca gggcgacgag ggccggagtg
     2941 cctgcctttt cgccgcgcat tttcgagccc ggcatcgatg gggcgttaag gacgctgcga
     3001 cgtttccagc gcctggcttc cctgcctttt tatcgcaggt acatggccgg atcccgattt
     3061 gggcccgccg gtcgcgcact ctgatataat cattgcggcg tcgttcgccc acgtcacggg
     3121 aatcgcgggg gatatcatgc ggataacact tgtcatcgtc tgtgcggcca tgttcgtttt
     3181 cctgtcttcc gtcgtcggct gcagccgcac gcatccggtt gatcccgaga tggtcatcct
     3241 tgacggcaag ccgatttcca cgatgttgcc gaccgcgcct cccccgcccg aggatcccga
     3301 ggccaggagg cgtgagcttc gaaagcacat tgctgacgac atgcggataa gatcgcttgc
     3361 gaacccggtt ggccgtggcg aattgcggcg cctttccggg ttccggtacg tcttccggga
     3421 agcggccccc ggcaccgtga agtcgattcc cgaactgcag gccgtcatcg gtgaactcca
     3481 gggcgccctt gaacaggcga aggtcgagca cggaaggcca ttcctgttca tccccgaatc
     3541 cgcgtcggga tggctgttca acgaggtcgt ccgggtctgc cttccgctgc cggccgatgc
     3601 ccaggcacct tccggcatgc cggtcgatga gttgaagacg acaatggtgg ttttccagcc
     3661 caggcgtacc cttccgcccg aaggaaagct tgacgagatg gagcgctggg cggagaaggc
     3721 acttgaggcg gcggcaggcg gcccggtcga tttcagcggg ccgttcatta tcaggacggc
     3781 gctcccggat ggattgcttt cggatcctga aggagttgac tggatggccc cctccgccgt
     3841 tgccgttccg gttccgggca gaaccgccga aggcggtgaa ccggccgaac ctgcggccga
     3901 ggaagacaag gttgagtgaa cagtcgtcaa gcaggctggt cgatctcgga atgcggctgg
     3961 ccgtgccata caatcacgac ccgcggctga tccagatgct tcagacgatg gccgcttcca
     4021 ttgccgagat cttcattccg gccccgcttg atgtcagcgg gacgttcagg gcctggaacg
     4081 gtccggatcc cgtcgagtat cgaaagatgg ttcccggcat cgtttgcgcc gcatcccgca
     4141 tcgggctgtc tgtcaacatg gcggtcaatt cccctttcgt tccgccttcc gaacacagga
     4201 tggttgccga ttacatcgcg aatgcggcat cggaaggcgt cagatcattc accatcgggg
     4261 atctgcatct tgccgagcat gtcagggccg tctgtcccga agtcgagctg gttgcgtccg
     4321 ccaccgcaga cattgcgcat gtgacgcggg ccaggcactg ggtcaggatg gccggcgttt
     4381 cccgcatcgt tccatcccgc gtggtgaaca aggatcccga tgtgctgcgg gcgatggcca
     4441 ggctgggcgt ccagatcgag attatcccga acgaacagtg cctgccggcc tgcccataca
     4501 tgttccagca tgccatgacc atcggcttca gggattcggt tgacgacacc gatttcagac
     4561 gttactgcgc catgtgcgcg gccatgaagc ccgcggcccc gtgggagcac tatcagacgg
     4621 agatcgttcc cgcgtccatc ccgcgctatc gcggcatcgt cagtcttgtc aagctcgccg
     4681 gccgcgattc gaccacgtcg ttcatcctgg gcgaagtcgg ccgcttcatc gcactggaat
     4741 cggattggac cggcttcctt ggcggctatc gcgaaccacc ggatgttttt gaaaagctgc
     4801 aaagctgcga tcgggtctgc gaggattgcg gctactgcca aagtgccttc atcgaggcca
     4861 atcccggctg gcaggatcgc ggatccgaat ggcttcggcg cgtaaccggc gtcgaatcag
     4921 gccgctgaac cgtccttccg gaatccttgt atttttgttg aaaatgatta tcattagcgc
     4981 gggccgtcga ggtggactgg gaatgaccct tctagatgtt tccgaatcgt tgcccgtaaa
     5041 ggtcgtatgc gtgggctgcg gcctcgggat gtcccgcaac ctggccagcc tgggcattca
     5101 cgcaggagat atcgtcagcg tcgtgcgccg cgccccgttt tcgggtccca tcctggtccg
     5161 ggttgaatcg actggagcga acattgcggt cggccgggag atggccggaa ggataacagt
     5221 tgaaactgaa agacataggg acgcttcttg aactgcccga ttccaacggt tccgacatac
     5281 cgaccctggc attcgtaggc cagcccaatt gcggaaagag cacccttttc aatgcccttg
     5341 ccggcttcaa ggccaacacg ggcaatttca cgggcaccag cgtcgagttc accgagagcc
     5401 tggccgtggt cggcaacagg cgcgtgcgcc tttttgacct tccaggaacc tattccctca
     5461 acccctcgga cgaggccgag gcggtcacca ggcgctggct ggacgaaagg cgcgccgacg
     5521 ccatcatcct cgtccttgac gcctcggttc tttccagaag cctggaactg gcactggaga
     5581 tggccgagac ggacatgccc ttcgcaattg ccttgaacat gatggacgac gctgccagga
//
LOCUS       DAOPPA010000179         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15719_length_5640_cov_3.393375, whole genome shotgun sequence.
ACCESSION   DAOPPA010000179 DAOPPA010000000
VERSION     DAOPPA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15719_length_5640_cov_3.393375"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKH54_06700"
     CDS             complement(<1..951)
                     /locus_tag="PKH54_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC99614.1"
                     /translation="MSRDIFDFGNVRWLLHCAEGPTPVEVAEAARRYMDLELRPWNRR
                     MQEDVIDILSGAKAEAAAVISRPGARAAQATDISLSQSTSTGLTLVARGFPFEPGDEI
                     IAPLGEFPSNVWPWKGLADSGVTFREVPLWDGHKSGREAWISTPPTAHDSPEERLAMA
                     IGPRTRMVTASWVRFQDGLRMDLGLLGRLCRDRGVPLVVDGIQGAGAMAPDLTDVSAF
                     ATGGHKGLLAMSGQGFLWTDPDFRKRLSPVGSWLSVEEGGNFNRAVTDFDRNWQENGH
                     KLEQGGYNVIGALVLRGSLGVINEVGSHNIETHIRGLQAGL"
     gene            1118..1744
                     /locus_tag="PKH54_06705"
     CDS             1118..1744
                     /locus_tag="PKH54_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99615.1"
                     /translation="MDGEPVKIPMDKILCGGKFFRLVAACCRNCTEPQFCREFWDFFQ
                     KNGITPQEYLRKFGIEEDGMKRIVFDCDRCGKKDIGEPVTVYYTEGANEGERMPTAEF
                     AAAIEKLGKVPVSKEFLGDVLVLLNTHENWEHYCSSCFRKVAQSAIGMLGPNAKSRKK
                     APASAESVMSAAGRAIAAKNQPKPVVLEPLDAPEPEVTRGRKKSGDQA"
     gene            1899..3941
                     /locus_tag="PKH54_06710"
     CDS             1899..3941
                     /locus_tag="PKH54_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99616.1"
                     /translation="MLNIRINFAAAALCCLVSLQAAGDPIVPEPIFPKESYPDLHQEM
                     ARHDRAYYRFLALPQGWSLEANLSSPDNLALINQFLDQSESEDVKAVTGKHPYEMFSN
                     YEEYGDLGMFGGVAVAATAFKYMTLKRENAPADQLAEARRDLVRAIETQHVFWQITGT
                     PGVVARGVQRLVDEDPEAPPIPRLNRNIVDLADGDGNPLPAPKNNGTWRADNSKGTLP
                     PDTWIWEDSCSKDQMIGQIFAMVLLYDAAVGDPDIDQALVTRMQQDALATAAMLMETR
                     DISTMEGPIGFGMYDLIIMDADGRPTMFHDFNPYSMEKIYVEAGSPGYNLFNLIMALG
                     VMKGLYHVTGSAEIETYIYQELMGNRNYLEMTTPPLENEEILDYILVELKTNFSNVNM
                     IATAIWLALYTESDPVVNGFLQKFMTERWWDKPGRTRTAKLSKQPFYNMVWMGLTDAG
                     VDRAMADQTAALLTAYKLGPYMNDAVINCDEAEIEAKQCLAVDGTTTLILEGGTNRTD
                     SPVATEALDPSIRPPSNFDARSDPFEANGGGGPRMNPGGDLLAAYWMGRFLDLNPAGV
                     SSKSPYVRTHIPVPGYEQVADPSPEASEEVASDVIEPEDQGPQAEVPGVDTGVGVDSV
                     VNDEGHQPADASIEQDTGSGKASGGCSAGDSPLAGPLVILSFLLVAFVALRMRFEA"
     gene            4048..>5640
                     /locus_tag="PKH54_06715"
     CDS             4048..>5640
                     /locus_tag="PKH54_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC99617.1"
                     /translation="MGFDIPALSKVWSVLTGTREQVRRDGYEGLEGTLIDGRFEVIRK
                     IGTGGMGSVYLARQKSMHRDVAIKVLNPDKVNDQDARERFRVEAAAVSRLKSPHTISV
                     YDFGEDERGGLFLVMELLQGRPLSEILERRGSLPIPMALEITDQILDSLSEAHRSGVL
                     HRDLKPENVFVREDLGGSVFVKVLDFGIAKVVGGPMESRTWAGSVFGTPAYMSPEQVM
                     GRELDVRTDLYAVAVVLYEMITGRLPITGETAIEVGIRKVRQRAPSLLQANPSVKCSP
                     EAIAFVAMALEPDRNRRPSSAEDFRKKMREAFRGAAFVERRRADAAGSGRQPAQAASG
                     STATPVVVSTADSVTAAWKYDGQTVSNAPDSIPAASVAAIWPGHAPEASVKVRTTSPE
                     KPATRPPDQVEALKAASEPLAAFDRRGRARLQRLATARCHYNGQVSQATVANSSASGA
                     FLHSTWLPVVGHRLTVSFVCPGSQVWGISVVAEVVRISSGTGAAGEVRGFAVRWLKMR
                     ARGSLSCMSKFLDGMMGIEFRSS"
BASE COUNT         1091 a         1671 c         1785 g         1093 t
ORIGIN      
        1 cagtccagcc tgcaggccgc ggatgtgcgt ttcgatgttg tgactgccga cctcgttgat
       61 gacgccaagc gagccgcgaa gaaccagcgc cccgatcacg ttgtagccgc cctgttccag
      121 cttgtggccg ttttcctgcc agttgcggtc gaaatcggtc acggcccggt tgaaattgcc
      181 gccttcctct acggacagcc acgatccgac cggcgaaagg cgcttcctga aatccggatc
      241 cgtccacagg aatccctggc ccgacatggc cagcaggccc ttgtgcccgc ccgtggcaaa
      301 cgcggacacg tcggtaagat ccggtgccat cgcccccgcg ccctgaatgc catcgacaac
      361 cagcggcacg ccgcgatcgc ggcacagacg ccccagcagc cccaggtcca tgcgcagccc
      421 gtcctggaac ctgacccagc ttgccgtgac catcctggtg cgcggcccga tcgccatcgc
      481 aagtctttcc tccggactgt cgtgtgcggt tggcggcgtc gatatccagg cttcgcgtcc
      541 cgacttgtgc ccgtcccaca ggggcacttc gcggaaggtc accccactgt cggccagccc
      601 cttccagggc cagacgttgg aagggaattc gccaagcggc gcgatgatct cgtcgcccgg
      661 ttcgaacggg aagccgcggg ccacgagtgt caggccggtc gatgtgcttt gtgaaaggct
      721 gatatcggtg gcctgggccg cccttgcccc cggtcttgaa atgacggccg ctgcctcggc
      781 tttggcgcca gacaggatgt cgattacgtc ttcctgcatc cggcggttcc agggccgaag
      841 ctccagatcc atgtagcgtc tggccgcctc ggccacctcg accggtgttg gcccctcggc
      901 gcagtgaagc agccagcgga cgttgccgaa atcgaaaatg tcacgactca ccggcgcacc
      961 catggtcatc accccggtcg ttcacggagc gggatcttat accgatacgc cggcttttgc
     1021 agggccgtgg cgaatgatga cgggaatcgt cgcgtatttc tgtctaaacg acgattcctg
     1081 aattccaaaa taagggcctt ctgctaggat tcggcgcatg gacggcgaac ccgtaaaaat
     1141 tccgatggac aagatacttt gcggggggaa gttcttccgt ctggtcgcgg cctgctgccg
     1201 caactgtacc gaacctcagt tctgccggga attctgggat ttcttccaga aaaacggtat
     1261 cactccgcag gaatacctgc gaaaattcgg catcgaggag gacggcatga agagaatcgt
     1321 cttcgactgt gaccgctgcg gcaagaagga catcggggaa ccggtgaccg tctattacac
     1381 cgaaggggcc aacgaagggg aaaggatgcc cacggcggaa ttcgccgcgg ccatcgagaa
     1441 actgggcaag gtcccggttt ccaaggaatt cctgggcgac gtgctggtgc tgctgaacac
     1501 ccacgaaaac tgggagcact actgttcgtc gtgcttccgg aaggttgccc agtcagccat
     1561 aggcatgctt ggcccgaacg ccaagtcgcg caagaaggcg ccggcatcgg ccgagtcggt
     1621 catgtcggca gccggaaggg cgattgccgc gaagaaccag ccgaagccgg tggttctgga
     1681 gccgttggat gcccccgaac ccgaggtcac ccgcggcagg aagaagtccg gcgatcaggc
     1741 ctgatgccac cgggaagcca gtggtgccgg ttctgcaagg ttcgtgctgt attcccggcg
     1801 ggcctttccg taatatgcct ccggccgttt ccggccgcct gatgccggcg gcgggatacc
     1861 cggtctcttt ttgtttcttt gtccccacgg ggtgaatgat gctcaatatc cgaatcaatt
     1921 tcgcggcggc ggcactttgc tgcctggtat cccttcaggc cgcgggcgat ccgatcgtgc
     1981 ccgagcctat cttcccgaag gaaagctatc ctgacctgca ccaggagatg gcgcgtcacg
     2041 acagggccta ctaccgtttc ctggccctgc ctcagggatg gtcgcttgag gccaacctgt
     2101 cgtccccgga caacctggcg ctgatcaacc agttccttga tcagtctgaa tcggaggacg
     2161 tcaaggcggt gaccggcaag catccgtacg agatgttttc gaattacgag gagtacggcg
     2221 acctgggcat gttcggcggg gtggctgtcg cggcgaccgc cttcaagtac atgacgctca
     2281 agcgcgaaaa tgccccggcc gaccagcttg ccgaggcacg tcgcgacctg gttcgggcca
     2341 tcgagacgca gcacgttttc tggcagatta ccggaacccc cggtgtcgtg gcccgtggcg
     2401 tccagaggct ggttgacgag gatccggagg cgccgccgat tcctcgcctg aatcgcaaca
     2461 tcgtcgatct tgccgacggc gacggcaatc cgctgccggc cccgaagaac aacggaacct
     2521 ggcgtgccga caattcgaag ggaaccctgc cgccggacac atggatctgg gaagactcct
     2581 gctcgaagga tcagatgatc ggccagattt tcgccatggt gctgctttac gacgcggccg
     2641 tcggcgaccc ggacatcgac caggcgctgg tgacccgcat gcagcaggat gcacttgcga
     2701 cggccgcgat gctgatggag acgcgggata tttccacgat ggaaggcccg ataggattcg
     2761 ggatgtacga ccttatcatc atggacgccg acggacgtcc gacgatgttc catgacttca
     2821 atccgtactc catggaaaag atttacgtcg aggccggaag ccccggatac aacctgttta
     2881 acctgatcat ggccctgggc gtgatgaagg ggctttacca cgtaaccggt tccgccgaga
     2941 tagagacgta tatctatcag gaactcatgg gcaaccggaa ctatctggag atgacgaccc
     3001 cgcccctgga aaacgaggag atccttgatt acatcctggt cgagctgaag acgaacttct
     3061 cgaacgtcaa catgattgcg accgcgatat ggctggcgct gtacaccgag tccgatcccg
     3121 tcgtgaacgg cttcctgcag aagttcatga ccgaaagatg gtgggacaag ccagggcgca
     3181 cgcgcacggc gaagctttca aagcagccct tctacaatat ggtgtggatg ggattgaccg
     3241 acgccggcgt ggatcgcgcc atggcggatc agaccgcggc cctgctgacc gcgtacaagc
     3301 tcggtccgta catgaacgat gcagtcatca attgtgacga ggcggagatt gaggcgaaac
     3361 agtgccttgc ggttgacggt acgaccactc tgatcctgga agggggaacc aaccggaccg
     3421 acagccccgt cgcgaccgag gcgcttgatc cgtcgatccg cccgccgtcc aatttcgacg
     3481 ccaggtccga tcctttcgaa gccaacggcg gcggcggccc caggatgaac ccgggcggcg
     3541 accttctggc agcctactgg atgggccgct ttctggacct gaatccggcc ggggtttcgt
     3601 cgaagtcgcc ttatgttcgt acccacattc ccgtacctgg ttacgaacag gtcgcggatc
     3661 cgtcgccgga ggcttccgaa gaggtcgcgt ccgacgtgat cgagccggaa gaccaaggcc
     3721 cgcaggcgga ggttccgggt gtcgataccg gcgttggagt cgatagtgtc gtcaacgatg
     3781 aaggtcatca gcccgcggac gcgtcgatcg aacaggatac cggctctggc aaggcttccg
     3841 gtggttgttc ggccggcgat tcaccgctgg ccggcccgct ggtcatcctg tcattccttc
     3901 tggtcgcctt cgtcgcctta cgcatgcggt ttgaagcgta aacaaagtta acaaatcaac
     3961 gcccggcacg cttgaccgat tccaagattg attccatcct atttggggac ggagtgtttc
     4021 gtccgcggct ttgattcccg gtggaacatg ggcttcgaca tcccggcgct ttcgaaggtc
     4081 tggagtgtcc ttacggggac acgtgaacag gttcgccgcg atggatacga gggccttgaa
     4141 ggcacgctga ttgacgggcg cttcgaggtc atccgcaaga ttggcacggg cggcatgggc
     4201 tccgtctatc tggccaggca gaagtcgatg catcgggacg tcgccatcaa ggtgctgaac
     4261 ccggacaagg tcaacgacca ggatgcgcgc gaacgtttcc gcgtggaggc cgccgccgta
     4321 agcaggttga agagtcctca caccatcagc gtatatgatt tcggcgagga cgaacggggc
     4381 gggttgtttc tggtgatgga actgcttcag gggcgtccgc tttcagaaat actggagcgc
     4441 cgtggttccc tgccgattcc gatggccctg gaaatcaccg accagattct tgattcgctt
     4501 tccgaagcgc atcggtcggg cgtcctgcac agggacttga agccggagaa cgtgtttgtc
     4561 cgtgaagatt taggtggcag cgtcttcgtg aaggttctgg actttggaat cgcgaaggtc
     4621 gtcggcggtc cgatggaatc acgtacgtgg gcgggcagcg tcttcgggac tcccgcatac
     4681 atgagcccgg aacaggtcat gggacgtgaa ctggacgtga gaacggatct gtacgctgtt
     4741 gcggtcgttc tttacgagat gattaccggt cgtctgccga taaccgggga gaccgcgatc
     4801 gaggtcggga ttcgcaaggt caggcagcgg gcgccgtccc tgctgcaggc caacccgtcc
     4861 gtgaaatgct cgcccgaggc aatcgcattc gtagccatgg cgcttgaacc ggacaggaac
     4921 aggcgaccgt caagcgccga agatttcagg aagaaaatgc gcgaggcatt ccgcggcgcg
     4981 gcgtttgttg aaaggcggcg ggcggacgca gccggttccg gcaggcagcc ggcgcaggcc
     5041 gcgtcaggtt caacggcgac tcccgttgta gtatcgacgg ccgactcggt gacggcggcg
     5101 tggaaatacg acgggcagac cgtctccaat gctcccgact ccattccggc cgcttcggtc
     5161 gcggcgattt ggccggggca cgcaccggaa gcgtcggtaa aggtcaggac gacatctccg
     5221 gaaaagccgg cgaccaggcc cccggatcag gtggaagcgt tgaaggccgc cagcgagcca
     5281 cttgcggcct ttgacagacg gggcagggcc aggctgcagc ggctggcaac cgcgcgttgt
     5341 cattacaacg gccaggtcag ccaggcaacg gttgcaaact ccagcgcatc cggggcattt
     5401 cttcattcta cctggcttcc cgtggtcggc caccgcctga ccgtgtcatt cgtctgtccc
     5461 ggttctcagg tatggggtat ttcggtggtg gccgaagtcg tgagaatttc ttccgggacg
     5521 ggcgctgctg gtgaagtgcg cggatttgct gtaaggtggc tgaagatgag ggccagggga
     5581 agcttgtcgt gcatgtcgaa gtttcttgac ggcatgatgg gtatcgagtt caggtcgtct
//
LOCUS       DAOPPA010000180         5617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15807_length_5617_cov_3.653901, whole genome shotgun sequence.
ACCESSION   DAOPPA010000180 DAOPPA010000000
VERSION     DAOPPA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_5617_cov_3.653901"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKH54_06720"
     CDS             <1..1224
                     /locus_tag="PKH54_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC99618.1"
                     /translation="ELAPEPGVVSGQPADQAAPPAASSEPVAPADEAAPPEMVEPSAE
                     AIDRARAFADQASALYDSGQFSEAIVQFNAALAIYEHPVIYFNLAKAYEKLAEYSPAA
                     EAYRQYLALTLKTTGEPAPDQADTERTIELLKEKAYLALPEVSIDSEPRGADVFIDGS
                     EALNGQTPMKTHLPEGKHSVTLKMDGYEPVSREFEVRSREPVRFSFGLVKERNEGAIL
                     VKVNIRQARIHVDGRVVGATPMLEPILIPPGRHQILIEREDYTSVSTVVDVKADETVE
                     VEAELHLSRKSFSWRGGVGVSAIILGAGIVGASFWGRYYINDDYKGQGMYNVVSPNAQ
                     SAPDFKMYKSLVYAGYGVGAGLAALGIGMLIWEVARKPAEVRDQDLIGSRKPSIRFMP
                     AFGPDSVGFGAVAAF"
     gene            1235..2311
                     /locus_tag="PKH54_06725"
     CDS             1235..2311
                     /locus_tag="PKH54_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC99619.1"
                     /translation="MKFFKAFLVVLVPLATACVVGIDPPPATERVLVTGGIFPFGSEL
                     VCTPASAVKERTCEDGLSGYPKVYPTVDVELKPFMADIHEVTNAQYRYCVEMGSCPLP
                     KFGNTQNIEFYWDNPMYADYPVINITQASAEAYCNFMDGRLPTEIEWERMAGGPGTLE
                     RPKRVYPAENLTWKMLSQCRSKLQDINVAFCANRGNPEEVMKSDNDYVLENGKKIFDL
                     AGNVAEWVSGGYVEGVTCQETLDECDCWACTDDNCRANCYTTCTACSEAGDACHVVCA
                     DAGLGLGLPVCLKYTDPVKPADLFEKGKNGMRLAKGGDYEVDVGQSCRVAVASRLRVF
                     KSIDDISPTTGFRCVADVATEGSE"
     gene            2308..3741
                     /gene="dacB"
                     /locus_tag="PKH54_06730"
     CDS             2308..3741
                     /gene="dacB"
                     /locus_tag="PKH54_06730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOC99620.1"
                     /translation="MIWNQHASLRFASFLVVILSAATVSAETDLPELQALAQSGLKVS
                     ALFSIDGKVVSSIDPDVRLNPASVTKLFSAAAAMKVLGPDYRMKTSVMRAGDDRLYIE
                     GSGDPDLWQKDLDELASCVASRGGKSKWSELVFSTGPFDRQDLPPAYDQKNTTAAYRA
                     GVAGLQIDQNAIRVAVGAAAPGQTLPVKVSPMSDYIRVDNQARASVEGKAGSRDRAKN
                     PLTIDVAPDASGRLVVTLRGLARPRRGSGYTFRVPNPAWNAAAAFVAALQKRKIKVAT
                     MVPRRGTIPEGAERICFRPSRAMREIIVPMLKDSINPVAESVLRLVGSVDSRAPVGFR
                     EGADRLRDFMFHEVLAPPESFVITNGSGLYGANSVSARAVVALVGWILATPEVKPHIR
                     AAMPVGGIDGTLAGRFTGSDLKGRVFAKTGTLDEAISLAGWVEMPDGRNLAFAVLVQG
                     DSKLNSAAVRSAIDVSVGRAFRAVSAP"
     gene            complement(3728..4492)
                     /locus_tag="PKH54_06735"
     CDS             complement(3728..4492)
                     /locus_tag="PKH54_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC99621.1"
                     /translation="MGFFSRLFGRNDTQATDAEQTPIKKVSPQIARLTKKLENKYGQA
                     QERQMAIDQLAEVGSVEAAQALLKRFSFRIEQTIGDEEEKRSVYDHLVALGPTSVQPI
                     LDYLNNENSPYWPTKALRQIVGDEETVTHLLDIIGSMEAIFDRDIQRKVELVSNLCEF
                     DHPEVIKALFEFAKDENEEIRVRAVEAMAKMGNEDLCDVLIDRLVDPAETQRMRVSIL
                     SLLVDRKWKVKHRKEEIRKVIPDSFWIDDVGTIKAR"
     gene            4703..5026
                     /locus_tag="PKH54_06740"
     CDS             4703..5026
                     /locus_tag="PKH54_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOC99622.1"
                     /translation="MVSDSPGKRVGKGTVFVKVEVCKGCAYCIEFCPADCLRFSRNYN
                     AKGYHYPVLAKPELCVGCNTCGQYCPDFAIMGMRYKDIDALEATKSAVNDDDRSGAEA
                     PGRKS"
     gene            5023..>5617
                     /locus_tag="PKH54_06745"
     CDS             5023..>5617
                     /locus_tag="PKH54_06745"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOC99623.1"
                     /translation="MKASPEGVLTGVHFMDGDHAACEGAIAAGCRFVAGYPITPSTEV
                     VERFSVRIPTVGGMFIQMEDEIASSIAIQGAAWGGAKVMTVTSGPGLSLMMEHIGLAV
                     MAEVPCVIVDVQRGGPSTGLPTLPAQADMMQVKWGSHGDYQIIALSPNSPQECFDLMI
                     RAFNLSETWRCPVFMMMDEVVGHMVERVEIPPADQIEI"
BASE COUNT         1090 a         1625 c         1734 g         1168 t
ORIGIN      
        1 gaactggcgc ccgagcctgg cgtggtttcc gggcagccgg cagatcaggc ggccccgccc
       61 gccgcttcct ccgagccggt agctccggca gatgaggcgg ctccaccgga aatggtcgaa
      121 ccgtcggccg aagcaattga ccgggcccgg gcgttcgccg accaggcatc ggccctttac
      181 gattccggcc agttctccga ggccatcgtt cagttcaacg ccgccctggc catctatgag
      241 catccagtca tctacttcaa cctggcgaag gcttacgaaa agcttgccga gtactccccg
      301 gccgccgagg cataccggca gtacctggcc ctgaccctga aaacgaccgg cgagccggct
      361 cccgatcagg ccgacacgga acgtacgatc gaattgctga aggaaaaggc atatctggcc
      421 ctgccggaag tcagcatcga ttccgagccc cgtggtgccg acgtctttat cgacggttcc
      481 gaggcactta acggacagac cccgatgaag acccaccttc cggaaggaaa gcacagcgtc
      541 accctgaaga tggacggtta cgagccggtt tcgagggagt tcgaagtcag gtcaagggaa
      601 cctgtcaggt tcagtttcgg gcttgtaaag gagcgcaacg agggcgccat cctggtcaag
      661 gtcaacatcc gccaggcacg cattcacgtg gatgggcgtg tagtcggcgc gacccccatg
      721 cttgagccga tcctgatccc tccgggccgt caccagatcc tgattgaaag ggaagactat
      781 acgtcggttt cgaccgtcgt cgatgtcaag gccgacgaga cggtcgaggt cgaggcggaa
      841 ctgcacctga gccggaagtc gttttcatgg cgcggcggcg tcggggtttc cgcaatcatc
      901 cttggggccg ggatcgtcgg tgcgtccttc tggggccggt attacatcaa tgacgactac
      961 aagggccagg gcatgtacaa cgtcgtgtcc ccgaacgccc agtccgcccc ggatttcaag
     1021 atgtacaaat cccttgtcta tgcgggctat ggcgtcggtg ccggcctggc ggcgcttgga
     1081 atcgggatgc tgatatggga agtcgcgcgc aagccggccg aggtcaggga tcaggacctg
     1141 ataggttcca ggaagccttc gattcgattc atgccggcct tcgggcccga ttcagtggga
     1201 tttggcgcgg tcgcggcgtt ctagggggtt tttaatgaaa tttttcaagg ctttcctggt
     1261 tgtcctggtg ccattggcga ccgcctgcgt ggtcggaatc gatccgcccc cggccaccga
     1321 acgcgtcctt gtaaccggcg ggatattccc tttcggttcg gaacttgtgt gcactccggc
     1381 aagcgccgtg aaggagcgta cctgcgagga tggcctgagc ggttatccaa aggtttaccc
     1441 gactgtcgat gtcgaattga agccgttcat ggcggatatc cacgaagtga cgaacgccca
     1501 gtaccgttat tgcgtcgaga tgggcagttg tccgcttccg aagttcggca acacccagaa
     1561 catcgagttt tactgggaca acccgatgta tgcggactat cccgtgataa acatcacgca
     1621 ggcctcggcc gaggcctact gcaatttcat ggacggacgt cttccgaccg aaatcgagtg
     1681 ggagcgcatg gccggtgggc cgggcactct cgagagacca aagcgggtct atccggccga
     1741 aaacctgaca tggaagatgc tgtctcaatg cagaagcaag ctgcaggata tcaatgttgc
     1801 attctgcgcg aaccggggca accccgaaga ggtgatgaaa tccgacaacg actatgtgct
     1861 ggaaaacggg aagaagattt ttgaccttgc cggcaatgtc gcggaatggg tttccggcgg
     1921 atacgtcgag ggagtcacct gtcaggaaac gctggacgaa tgcgattgct gggcgtgcac
     1981 ggacgacaac tgcagggcca actgctacac cacatgcacg gcctgcagcg aggcgggcga
     2041 tgcatgccac gtcgtctgcg ccgacgcagg ccttggactc ggcctgccgg tctgcctgaa
     2101 atacaccgac cctgtcaagc ccgccgattt gttcgagaag ggaaaaaacg ggatgcggct
     2161 tgcaaagggc ggcgactatg aagttgatgt cggacagtcg tgccgcgttg cggtcgcctc
     2221 tcggctgcgg gtgttcaaaa gtatcgacga catcagtccg accaccggat tccgctgcgt
     2281 cgccgatgtc gccaccgaag gatccgaatg atctggaacc agcacgcatc attacgtttc
     2341 gcgtcattcc ttgtcgtaat cctgtcggcg gcgacggtca gcgccgagac cgatttgccc
     2401 gagctgcagg ctcttgcgca atcgggattg aaggtcagcg cactcttttc catcgatgga
     2461 aaggtcgttt cgtcgattga tcccgatgtc aggctgaatc cggcgtccgt gacgaagctt
     2521 ttttccgccg cggccgcaat gaaggtcctg gggcctgatt accgcatgaa gacgtcggtc
     2581 atgcgcgccg gtgacgaccg cctttacatt gaaggaagcg gggatcccga tctgtggcag
     2641 aaggatctcg atgaactggc ttcgtgcgtt gccagccggg ggggcaagtc gaagtggtcc
     2701 gaattggtat tttccaccgg gccgtttgat cgtcaggatc ttccgccggc ctatgaccag
     2761 aagaacacga cggcggccta cagggcgggc gtggccgggc ttcagattga tcagaacgcg
     2821 ataagggtcg cggtcggcgc tgcggccccc gggcagactc ttcccgtgaa ggtcagcccg
     2881 atgtcggatt acatccgcgt cgataaccag gcacgtgctt ctgtcgaggg aaaggccggt
     2941 tcgcgtgacc gggcaaagaa tccattgaca atcgacgtcg cgccggacgc cagcgggcgc
     3001 cttgtcgtga cgctgcgggg gcttgcaagg ccgcgcaggg gatccggata caccttccgc
     3061 gtcccgaacc cggcgtggaa cgcggcagcg gcctttgtcg cggcgcttca gaaaaggaag
     3121 atcaaggtcg caacgatggt tccccggcgt ggaacgattc ccgaaggcgc cgagcggata
     3181 tgttttcgac cttcccgcgc gatgcgcgag ataattgtcc cgatgctgaa ggacagcatc
     3241 aaccctgttg ccgagtcggt tctgcgcctt gtcggatccg tggattcgcg tgcgccggtc
     3301 ggtttccggg agggggcgga caggctgcgt gacttcatgt ttcacgaagt gctggccccg
     3361 ccggaatctt tcgtcatcac aaacggttcc ggtctttatg gcgcaaattc ggtcagtgcg
     3421 cgcgcggttg tggcccttgt cgggtggatc ctcgccacac ccgaagtcaa gccgcacatc
     3481 agggccgcga tgccggtcgg tggaattgac ggaaccctgg ccggccgttt caccggttcc
     3541 gacctgaagg gccgggtttt tgcaaagacc ggaacgcttg atgaagcgat ttcacttgcc
     3601 ggatgggtcg aaatgccgga cggcaggaac cttgccttcg ccgttctcgt ccagggcgat
     3661 tcaaagctga actcggctgc ggtcagatcg gcgatagacg tgtcggtcgg cagggccttc
     3721 agagccgtca gcgcgccttg atggtgccga cgtcgtcgat ccagaacgaa tccggaatga
     3781 ccttgcggat ttcttccttt cggtgcttga ccttccattt gcggtcaacc agcaggctca
     3841 ggattgaaac cctcatcctc tgggtctcgg ccggatccac caggcggtcg atgagcacgt
     3901 cgcaaagatc ctcgtttccc atcttagcca tggcctcgac agccctcacg cggatttcct
     3961 cgttttcatc cttcgcgaac tcgaaaagtg ccttgatcac ttcagggtga tcgaattcgc
     4021 agagattgga aaccagttca accttgcgct ggatgtcccg gtcgaaaata gcttccatcg
     4081 aaccgatgat gtccagcagg tgggtaaccg tttcctcgtc gccgacgatc tggcggagtg
     4141 cctttgtcgg ccagtacggt gaattctcgt tgttcaggta atccaggatc ggctgtaccg
     4201 acgtcggtcc gagcgccacc aggtggtcat agacgctgcg cttttcctcc tcgtccccaa
     4261 tggtctgctc gattcggaag ctgaatcgct tcagcagggc ctgggccgct tcgacacttc
     4321 cgacctcggc gagctggtcg atcgccatct ggcgctcctg tgcctggccg tacttgtttt
     4381 ccaacttctt cgtaagccgt gcaatctgcg gcgagacctt ctttatcggc gtctgttccg
     4441 cgtcagtcgc ctgagtatcg ttacgtccga aaagcctgga aaaaaaaccc atcaggatcc
     4501 tcctgttctg gccgcacaga caggtaccag aaaacggcga ggttttcaat ctgtgcaaag
     4561 cgtcccgaat aaacttgact ttaacttcca aaaggttaac ttgcggggcg aggccccgga
     4621 tggcgcgcct gacaggcttc ggccgggcac tggccgcctt tttgaaggga tggggtcgcg
     4681 tggctcgaac tggaggtttg ccttggtttc cgacagcccc gggaagcggg ttggcaaggg
     4741 gacggtgttc gttaaggtgg aggtgtgcaa ggggtgcgcc tattgcatcg agttctgccc
     4801 tgccgactgt cttcgctttt cccgtaacta caacgcaaaa ggttatcatt acccggtact
     4861 tgcgaagccc gaactgtgcg tcggctgtaa tacctgcggc cagtactgtc cagatttcgc
     4921 catcatggga atgaggtaca aggatatcga tgccctcgag gcgacgaagt ccgccgtcaa
     4981 tgacgacgac aggtcaggag ctgaagcccc ggggaggaag tcatgaaagc cagtcccgaa
     5041 ggagtcctga ccggtgttca cttcatggac ggcgatcacg ccgcctgcga aggggccatc
     5101 gccgccggct gccgctttgt cgccggctat ccgatcacgc catccacaga ggtggtcgag
     5161 cgtttttcag tcagaatccc gaccgttggc ggcatgttca tccagatgga agatgaaatc
     5221 gcgtcgtcga ttgcgatcca gggtgccgcc tggggcgggg ccaaggtcat gacggtcact
     5281 tccggccccg ggctttccct gatgatggag catatcggtc ttgccgtaat ggccgaagtc
     5341 ccgtgcgtca tcgtcgatgt ccagcgtggc ggtccttcaa ccggtctgcc gacgctgccg
     5401 gcgcaggccg acatgatgca ggtcaagtgg ggctcccacg gcgattacca gataatcgcg
     5461 ctgtccccga attcccccca ggaatgcttc gacctgatga ttcgcgcatt caacctttcc
     5521 gagacatggc gctgcccggt tttcatgatg atggacgagg ttgtcggcca catggtcgag
     5581 cgtgtcgaga taccgccggc ggaccagatc gagattg
//
LOCUS       DAOPPA010000181         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15827_length_5613_cov_4.000360, whole genome shotgun sequence.
ACCESSION   DAOPPA010000181 DAOPPA010000000
VERSION     DAOPPA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15827_length_5613_cov_4.000360"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKH54_06750"
     CDS             <1..346
                     /locus_tag="PKH54_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99624.1"
                     /translation="FEPDPRNPELPYVRDVQQRRLEILVGAGGSGRLELADGTVIAAS
                     GDSVTTVPTGIAECGQDESQWDTDCFVRSGQFTLISLRGNSAVELAGMKVSVTGGPST
                     RRYVVRVFGSAI"
     gene            438..3125
                     /locus_tag="PKH54_06755"
     CDS             438..3125
                     /locus_tag="PKH54_06755"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOC99625.1"
                     /translation="MKTVSLLLLSVLLVVSDGCRSPQQVDSDSGVDTGSRPDLPPEVD
                     PGRWEGLVINEVAPKGVPADWFELFNTGLKPIPLDGFSVSDDSGDRSDAVPFPSGLVV
                     PAGDRLLLQFDDDWPGFGLGVDEELSILAPDGTVIDSTGWLSIAAVPGRSWGRVPDGT
                     GSFLVMDCPSPGRENTEATCEASLPSDILFQKTAVVEVELTLPVESVTSLNLDGSRWV
                     DSKVRLRVGDFDSGTLDAGVRLKGRWGSAQTLDGKAAFKIKFDHSVADQDVLGMEDLT
                     LNNMVQDCTMLHEHLSYELFRAFDIPVPRTGWAVVSVNGEPYGVYLLLEKYDRRFFNR
                     FYPDTAHVFEGAYGSDVLPGQERYFEIKDGDSADVSDLSALASAVAEPDDRKWQARLS
                     GVAAVDEMMRVWAVEQYIGHWDGYAPTINNFFLRCDSNGIFTLHPWGLDQTWGDFRDY
                     HSGSGAIFARCMEIPECRARYDKVMLDLLNVVEQLDVIRMAEALDRFIESYRTADRRR
                     ICMEDQGHLEWTMWFIAARQRDARMRVPCFVDSTTDQDDDGYRCEEDCDDLNEKINPG
                     VDDACFDWIDQDCSGLHDDGPGCPDCRAIWRGPHRYAYCTVKRSVADAVSACRDAGLA
                     LVNLNSESEQDWLLAQMPPVGMRSFWTGLEKIDGVFVWADGSQPGWTSWAPGEPSECG
                     ECSCSILDDSGAWLAVDCAAEHNVLCEAACADPGDSDGDGFGACADDCDDGNRDIHPG
                     AVDVCGDWVDQDCDGMTDGLPECYGAFDVPVDSAIARGDRFVLHVFQAEFETARILCG
                     KYGPEAGLAWFDEPGQEAAVAEIVRAWLGPARFLIGLNDRNEEGRWLWLAGGSPAYSN
                     WGPSQPDRGERADCVENGPDGFWNDIDCVQPRQFVCRVP"
     gene            3367..4728
                     /gene="rsxC"
                     /locus_tag="PKH54_06760"
     CDS             3367..4728
                     /gene="rsxC"
                     /locus_tag="PKH54_06760"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOC99626.1"
                     /translation="MAVRKTFKGGVHPHEYKELSCSKGSVEMPAPSRVVVPLRQHLGA
                     PAKAVVNVGDRVVEGQVIGEPGGFVSAPVHAPIAGEVTAVAKHRHPGGFVCDAIVIEA
                     KEPVPGPDGNVPVQVPYRMPAAVGDRYMEADPALLKNLIRDAGLVGMGGAAFPTHVKL
                     SPPAGKPVDTLVINGAECEPFLTCDHRVMLEETEKVVHGVRILMRVLGVERCFVGIED
                     NKPDAIAQLSAAFADVPGVVVSACRVKYPQGGEKQLIKALTGREVPPPPGLPLDVGCV
                     VQNVGTAARVAEAVMEGRTFTSRVITLSGTQIANPMNVRVKVGTMLADVVEFCGGLKS
                     DLSRVIQGGPMMGIAMADLQVPVVKGTSGFVFLEAGNPGDYQAASCIRCGRCVSACAL
                     RLQPVEIAKKLEAGDLEGAEALHVMECMECGSCSFACPSNRWLVQLFRIAKGKINERR
                     RKA"
     gene            4706..>5613
                     /locus_tag="PKH54_06765"
     CDS             4706..>5613
                     /locus_tag="PKH54_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOC99627.1"
                     /translation="MSADAKLDTGKAAAAPERLIVSPSPHLRSKDTTASIMMWVAIAL
                     LPGVAVSFYYFGLEAVRTMVIAVVSCLAFEFVGMKLLGTAGSLKDMSALVTGILLGMN
                     LPPTAPWWMVAAGGLIAMVVAKHLFGGLGSNVFNPALTARVFLLIAWPVQMTTWLKPG
                     TTGLVTAEGLQAVTGATPLAIIKEGGVGALSGTGAIDMLMGNVGGSLGEVSAAALLLG
                     GILLLAKRIITWEIPVFFIGTTALVTGIAWMANPAQYASPLTHVLTGGLMLLMKEEHR
                     RTKLVLNIASTTLGLVIAVALLMWVKR"
BASE COUNT         1002 a         1548 c         1829 g         1234 t
ORIGIN      
        1 gttcgagccg gatccgcgta atccggaact gccatacgtt cgtgatgtgc agcagcgcag
       61 gttggaaata ctggtcggcg ctggcggcag cggccgtctt gaactggccg acggcacggt
      121 gattgccgct tcgggcgatt ccgtgaccac tgtcccgacc ggcattgcgg agtgcggcca
      181 ggatgaaagc caatgggata cggactgctt cgtcagatcc ggtcagttca cgctgatttc
      241 attgaggggc aattccgccg tcgaactggc agggatgaag gtttcggtaa ccggcggacc
      301 gtccacaaga cgatatgtcg tccgggtctt cggcagcgcg atttgattgt tctgcgcgag
      361 tgaatgcatt cggtcaatct ttatgattgt cgttgtatga tcgtctcaac gcccgcaaat
      421 ccgggaggat tcttcaggtg aaaacggtct ccctgcttct gctttccgtc cttctggtcg
      481 tttctgacgg ctgtcggtcc ccgcagcagg tcgattccga ctccggggtg gatacgggga
      541 gccggcccga cctgccgcct gaagtcgatc ctggccggtg ggaaggcctg gtgatcaacg
      601 aagttgcgcc gaagggcgtt cccgcggact ggtttgagct ttttaacaca ggccttaagc
      661 cgatcccgct tgacgggttc agtgtgtcgg acgattcggg ggatcgttcc gatgctgttc
      721 cctttccttc tggccttgtc gttcccgccg gcgaccgcct tctgttgcag tttgatgatg
      781 attggccggg gtttggactg ggggttgacg aggaattgag catcctggcc ccggatggga
      841 ccgtgatcga cagcaccgga tggttgtcga tcgccgcagt tccggggaga tcctggggca
      901 gagtcccgga tggtaccgga tcttttctgg ttatggactg tccttctcca ggtcgggaga
      961 acacggaggc cacctgcgaa gcatcacttc cgtcggacat tctttttcaa aaaacggcag
     1021 tcgttgaagt cgaacttacc ctgccagtcg aatcggtgac ttcgctcaat ctggatggaa
     1081 gtcggtgggt cgattcgaag gtcaggcttc gtgtcggcga cttcgacagc gggactcttg
     1141 atgctggcgt caggctgaag ggacgctggg ggtcggcaca gaccctggat ggaaaggcgg
     1201 cattcaaaat caagtttgac cactcggtgg cagatcagga tgtgctcggc atggaggatc
     1261 tcacacttaa caacatggtt caggattgca ccatgcttca cgagcacctc tcgtacgaac
     1321 ttttccgggc gttcgacatc cccgttccca ggactggttg ggccgtggtt tcggtaaacg
     1381 gggagccata cggggtgtac cttctccttg aaaagtacga cagacggttc ttcaatcgct
     1441 tctatcctga tacggcacat gtttttgaag gtgcctatgg cagtgatgtc cttccggggc
     1501 aggaacgcta tttcgagatc aaggatggag attccgccga tgtctcggat ttgtccgcgc
     1561 tggcatctgc agtcgcggaa cctgatgacc gcaaatggca agcccgtctt tcaggtgtgg
     1621 ctgccgttga cgagatgatg agggtctggg cggtcgagca atacatcggt cactgggacg
     1681 gttacgcacc gacaatcaac aatttcttcc tgcggtgcga cagtaatggg attttcaccc
     1741 tgcatccctg gggacttgat cagacgtggg gcgattttcg ggactaccat tccggttccg
     1801 gcgccatatt tgccaggtgc atggagattc ccgagtgcag ggccaggtat gacaaggtca
     1861 tgcttgattt gctgaacgtt gttgaacaac tggacgttat ccgcatggcc gaagcgctcg
     1921 atagattcat tgaatcgtac cggacggccg atcggcggcg gatctgcatg gaggatcagg
     1981 gccatctcga gtggaccatg tggttcatcg cagcgcgtca gcgggatgca aggatgaggg
     2041 tgccatgctt cgtggattcg accaccgatc aggacgatga tggatatcgt tgcgaagagg
     2101 attgcgacga ccttaatgaa aaaatcaacc caggtgtcga tgatgcctgc tttgactgga
     2161 tagaccagga ttgcagcggg cttcatgacg acggtcccgg ttgtccggac tgccgcgcca
     2221 tctggcgcgg acctcaccgt tatgcctact gtaccgtgaa acgcagcgtg gctgatgccg
     2281 tctcggcgtg ccgggacgcc ggactggctc tggtgaatct taactcggaa tctgaacagg
     2341 attggttgct ggcgcagatg cccccggtcg ggatgaggtc cttctggacg ggccttgaaa
     2401 agatagacgg agtcttcgtt tgggcggacg gaagtcagcc aggctggact tcctgggcgc
     2461 ccggcgaacc gtccgaatgc ggtgaatgct cctgttcgat cctcgacgat tccggcgcct
     2521 ggctggcggt ggattgtgca gcggaacata acgtcctttg cgaggcagcc tgcgccgatc
     2581 cgggcgattc tgacggcgat ggtttcgggg cctgtgctga cgactgtgat gatggcaaca
     2641 gggatatcca tccgggcgcg gttgacgtct gcggagactg ggtcgatcag gattgcgatg
     2701 ggatgacgga cggtctgccg gagtgctacg gggcgtttga cgtcccggtt gactccgcga
     2761 ttgcccgggg ggatcgcttc gtccttcacg tgtttcaggc cgagtttgaa acggcacgaa
     2821 tcctgtgcgg gaagtatggg cccgaagccg gacttgcctg gttcgacgag ccaggtcagg
     2881 aggctgccgt ggcggagatt gttcgagcct ggctggggcc ggcccggttc ctgatcggtt
     2941 tgaatgaccg gaatgaagag gggcgatggc tctggctcgc agggggatcg cccgcatatt
     3001 ccaattgggg cccaagccag ccagatcgtg gtgagcgtgc agattgcgtt gaaaatggac
     3061 ctgacggttt ctggaacgat atcgattgcg tccagccgcg tcagtttgtc tgcagggttc
     3121 cgtgactcat cccgattcgg tgtcagcggt caggacatct tgcgctgtta gaaacttcac
     3181 agcagcccgt tggcgatctg tcaaggtttt tcgaaataac ttgttcgttt tcaagcggtt
     3241 taggggtggc attttacccg ccaggggata aaatgccgcc gcgcgggcgg gacgtccgca
     3301 agcgtgtccg gcaaggtcgg gcgattccgg catcactcgg ccggatcatc tctttcgggg
     3361 gttcttatgg cggtaagaaa gaccttcaag ggtggcgttc atccccacga gtacaaggaa
     3421 ctgtcgtgct cgaaggggag tgtcgaaatg ccagccccgt ccagggtggt cgtgcctctc
     3481 cgtcagcatc tcggggctcc ggcgaaggcc gtcgtcaatg tcggcgaccg cgtcgtcgaa
     3541 ggccaggtca tcggcgaacc cggcgggttc gtttccgcgc ctgtccacgc cccgattgcc
     3601 ggcgaagtaa cggccgtggc gaagcatcgc catccgggcg gatttgtctg cgacgcgatt
     3661 gttatcgaag cgaaggaacc ggttcccgga cctgacggca atgttccggt gcaggttccc
     3721 taccgcatgc cggccgccgt cggcgaccga tacatggaag cggatccggc cctgctgaag
     3781 aacctgatac gtgacgctgg tcttgtcggc atgggcggtg cggccttccc gacacatgtg
     3841 aagctgtccc cgccggccgg caagccggtt gatacgctgg taatcaacgg cgccgaatgc
     3901 gaaccgttcc tgacctgcga tcaccgcgtc atgctggaag aaaccgaaaa ggtcgtccac
     3961 ggcgtccgca tactgatgcg cgtcctgggc gtcgaacgct gcttcgtcgg tatcgaggac
     4021 aacaagcccg acgccatcgc ccagctttcg gcggcctttg ccgacgttcc gggcgtggtc
     4081 gtttccgcat gcagggtcaa gtacccgcag ggcggcgaaa agcagctcat caaggcgctg
     4141 acgggacgtg aagtcccgcc gccccccggg ctgccgcttg acgtcggctg cgtcgtccag
     4201 aatgtcggta ccgccgcgcg cgtcgccgag gcggtcatgg aaggccgcac gttcaccagt
     4261 cgcgtgatta cgctttcggg cacgcagatt gcgaacccga tgaacgtccg cgtcaaggtc
     4321 ggcacgatgc ttgccgacgt ggtcgagttc tgcggtggcc tgaaatcgga tctgtcccgg
     4381 gtaatccagg gcggaccgat gatgggcatc gcgatggcgg accttcaggt cccggtggtc
     4441 aagggcacgt ccggcttcgt attcctggaa gcaggcaacc cgggcgacta ccaggcggcc
     4501 tcgtgcatcc gctgcggacg ctgcgtaagc gcgtgcgccc tgcggcttca gccggtcgag
     4561 atcgcgaaga aactggaagc cggcgacctt gaaggcgccg aggcccttca cgtaatggaa
     4621 tgcatggaat gcggttcgtg cagtttcgcc tgtccgtcga accgctggct tgtgcagctt
     4681 ttccgtattg ccaaggggaa gatcaatgag cgcagacgca aagcttgata ccggtaaggc
     4741 tgccgctgcc ccggaacgtc tgatcgtaag tccttcaccg caccttcggt cgaaggacac
     4801 gacggcatcg ataatgatgt gggtcgcgat cgcgctgctt cccggtgtgg cggtatcttt
     4861 ttactacttc ggcctggaag ccgtgcgtac gatggtcatc gccgtcgttt catgcctggc
     4921 tttcgagttt gtcggcatga agctgcttgg caccgcgggc agcctgaagg atatgtccgc
     4981 gctggtcacc ggcatccttc tgggcatgaa ccttccgccg accgctccct ggtggatggt
     5041 cgcggctggc ggcttgatcg cgatggttgt ggccaagcac cttttcggcg gtcttggaag
     5101 caatgttttt aatccggcgt tgactgcccg cgtatttctg ctgattgcct ggccggttca
     5161 gatgacgacc tggctgaagc cgggaaccac cggcctggtt acggccgagg ggctgcaggc
     5221 cgttaccggc gccacccccc tggcgatcat caaggaaggc ggcgtcggtg ccctgtcagg
     5281 aaccggtgcc atcgacatgt tgatgggcaa cgtgggcggt tcgcttggtg aagtctcggc
     5341 cgcggccctg ctgctgggcg ggattctgct tctggcgaaa cggattatca cctgggaaat
     5401 cccggtgttt ttcatcggca cgacggcgct tgtgaccgga atcgcctgga tggcgaatcc
     5461 ggcgcagtat gcaagtcccc tgacccatgt cctgaccggc ggcctgatgc tgctgatgaa
     5521 agaagagcac cgccggacca agctggtgct caacatcgcc tccaccacgc tgggcctggt
     5581 gatcgccgtg gccctgctga tgtgggtgaa gcg
//
LOCUS       DAOPPA010000182         5595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15880_length_5595_cov_4.546209, whole genome shotgun sequence.
ACCESSION   DAOPPA010000182 DAOPPA010000000
VERSION     DAOPPA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15880_length_5595_cov_4.546209"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKH54_06770"
     CDS             <1..237
                     /locus_tag="PKH54_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99628.1"
                     /translation="QVPADPAASAAVSQVFRDLTAHFALVSKRSVAQGDYMDFAYQVR
                     FRKPQDCDDLLARLEGIDGMKGLVYVNEQTTVEV"
     gene            243..2348
                     /locus_tag="PKH54_06775"
     CDS             243..2348
                     /locus_tag="PKH54_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOC99629.1"
                     /translation="MKMKLDRRYLFWAFWALAGVAVLSIYFRYETNSDFIGLVESRTH
                     VLGVREEGRIAVIHVVQGQNVAAGQVLLELDMSDLDAEEALLRAELSRREAQLNADRI
                     RFGLEFDKLLTQREADALNIYLKHTDLVEKKAELDSVKQEIARLQSAQDAGLGRSRDL
                     SDLIIRRDTLERVIAAQKPILKSKGAGSGDDGRDKVAEREEVVRSMLGENHEKMNEIR
                     VNLQIVQARRANRTIISPTDGRVVNLVNMAGDTVDNFVPVINIEETAINYVDAYVPET
                     SGQLPQIGQRVNLYPHRQMAQNATGVIVAVDPGYQAIPLRLSFRGNLYWARKFRIQVD
                     RPHALMPGEAVRVEMQDSMIGVTDAIANAIDHQKAPGTRQPDAAPASGDVPMVETSGS
                     TVTLDASALQKIRYAPASGFEGSFEPSGLIWLADIERYLIVSDDTGAGKARHSPWLFL
                     MDRFGAVDPKPVVLQGVAELNDLEAITASPDGGIYLVSSNNANRKGKRKPVRQQILKV
                     AREGRDFRVVASAVLFDAVMQLGPEQVAALGIVKDGSGLDVLNIEGASWDLAGSEPAM
                     LIGLKQPVGPEGSIIWRLKNPDLFLASGKLAPGQLSVFAKVDLGRVRERPASISDLLV
                     TADGIYVLSTVPDAPDPDQIGRLLRLQPVQGGYVASVLFEYRGLKPEGLCVTEGDSFT
                     VVMDTGDAPALFHTSRWSR"
     gene            2345..3667
                     /locus_tag="PKH54_06780"
     CDS             2345..3667
                     /locus_tag="PKH54_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC99630.1"
                     /translation="MIWRGAAVILVVLLVPPLTAVALESFGDDAKGSPAPGANFDPAS
                     DDGFSGLVMSEGDVVQAAVDSSPELQRLRQRASARKVGADFAKYDVRNPELRIQDLST
                     EYFDSDANHRFQIGLRWHLPRFGEVGENVALARWEYQDACVDEFEYRVQVIGQARQAF
                     FNVLLLKDEVAAAQRREALEARRVDVLSRLADLGTVPFVRSMKAQTSLLKARRDVASL
                     QKALGAARQALAAIVGNDSPVDAAGFELPEGEPQLDAIMKRAESVRPELALEARRTEL
                     ARASRRAEVLDMIPTFRFVQAAYHYESRDRDWGELSFGIEIPVFNWGRHKLRRAEMAL
                     ASREDNGSAGIDQVRKRIGQDFAQASAARAAWVTLKDHVAKAREDVERMAAVARSKGI
                     SEEEVIDLELSGAELEWMMVDARRDYVEALLDLCEAAGFDNFGELLGE"
     gene            3680..4513
                     /locus_tag="PKH54_06785"
     CDS             3680..4513
                     /locus_tag="PKH54_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOC99631.1"
                     /translation="MEGNRTEVISRYESKYLVPSSVVPAMRDYISAFCSPDRHVDPDG
                     RYVVNNLYFETPDLRFYNDTRLKRFTRFKPRCRFYGDGSDGFLWLELKHKVANVTWKI
                     RRRIPVGGWDDLFMANRFEPGRVEPGLNGARTAARRVKVVESFEDAVITFGAAPFLFV
                     KYQREPYVSDVNAYARITFDRQLSYCMAEGSSKLDHGRQYIYYDDGATALHVEDESPV
                     LVEIKTETSVPTWVMGMIGRFDLSRRGFSKYCQAVDHFMCFNRPQARNPAIDLSRRLF
                     R"
     gene            4510..>5595
                     /locus_tag="PKH54_06790"
     CDS             4510..>5595
                     /locus_tag="PKH54_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOC99632.1"
                     /translation="MKNAVSVAFFLSLLVAFVGCGGDSNPVGDVTDDVVPVESEVRLN
                     EIDCHGTDWIEIVNVGDMVADLAGWIVTDGLDKTDHYYTLPAGSIVRPGGYLVVKGVS
                     ETREGFDFGIKCDDESVFLMDPSKKVIDVTDVTAVPDGFTWGRSPDGTGAFTVTLPTQ
                     GTANIENPDTPDELFDPGVVNLFEITLGSDAVSGLTTDPYEYVSGVVSVTVAGKTVSG
                     QPVGVRLKSGYSFQPLTAKAAFKIKADGYAVGDRVLGMKNFTLNNMVDDRTMMHETLA
                     YKLFREAGIPAPRTGYAQVRVNGQDYGLYVLVENYDDQFTTFNFASTQHLYEGTGDLY
                     AGQIGKFDVEDGDKLDVADLEKLMERIA"
BASE COUNT         1104 a         1573 c         1802 g         1116 t
ORIGIN      
        1 caggtgcccg ccgacccggc ggcatcggct gccgtctcgc aggtcttccg cgacctgacg
       61 gcccacttcg cccttgtttc gaagcgcagc gttgcccagg gcgactacat ggacttcgcg
      121 taccaggttc gcttcaggaa gcctcaggat tgcgacgacc ttctggcgcg gctcgaggga
      181 atcgacggca tgaagggcct tgtctatgtg aacgaacaga cgacggttga ggtctgaccg
      241 atatgaagat gaaactggac cgaaggtacc tcttctgggc gttctgggcg cttgccggcg
      301 tggcggtgct ttcaatctat ttcaggtacg aaacgaactc cgacttcatc ggactggtgg
      361 aaagccgcac ccacgtgctc ggggttcgcg aggaaggccg gatcgccgtg attcacgtgg
      421 ttcagggcca gaacgtggcg gcgggccagg tgctgcttga actggacatg tccgaccttg
      481 acgcggaaga ggcgctcctg cgcgccgagc tatctcgtcg cgaggcccag ttgaacgcgg
      541 atcggatccg tttcgggctg gaattcgaca agctgctgac ccagcgcgag gccgatgccc
      601 tgaacatcta cctgaagcat accgacctgg ttgaaaagaa ggccgaactc gattcggtaa
      661 aacaggaaat cgccaggttg cagtcggccc aggatgcagg gcttggcaga tccagggatc
      721 tttcggattt gataatacgt cgtgacaccc tggaaagggt gatcgccgcg cagaagccga
      781 tcctgaaatc aaagggggcc ggttcgggcg atgatgggcg cgacaaggtc gcggagcgtg
      841 aggaagtcgt ccggtcgatg cttggcgaaa atcacgaaaa gatgaacgaa atcagggtta
      901 acctgcagat tgtgcaggcc aggcgggcca accgcaccat catttccccg acggacggtc
      961 gcgtggtgaa cctggtgaac atggcgggcg acacggtcga taacttcgtt ccggtcataa
     1021 acatcgagga aaccgcgata aattatgtcg atgcgtacgt tcccgagacc tccggccagc
     1081 ttccgcagat cggtcagcgc gtcaacctgt atccgcaccg tcagatggcc cagaacgcta
     1141 ccggcgtgat cgtggccgtc gatccaggtt accaggcaat tccgcttcgt ctttcgttcc
     1201 gcggcaacct ttactgggcc cggaagttca ggatccaggt cgatcggccg cacgcgctga
     1261 tgccgggcga agccgtccga gtcgagatgc aggattccat gatcggcgtc accgacgcaa
     1321 tagcgaacgc aatcgatcat cagaaggcgc ctggaaccag gcagcccgat gcggccccgg
     1381 ccagcggcga cgtgccgatg gtcgagacgt ccggttcgac ggtgaccctg gatgcgtcgg
     1441 cccttcagaa gatccggtac gccccggcat ccggctttga aggcagtttc gagccttccg
     1501 ggcttatctg gctggccgac atcgaaagat acctgatcgt cagcgacgac accggtgccg
     1561 ggaaggcccg tcattcgcca tggctcttcc tgatggatcg tttcggtgcg gtcgatccga
     1621 agcctgtcgt ccttcagggt gtcgcggaac tcaacgacct ggaggctatt accgcatcgc
     1681 ccgacggcgg catatacctg gtcagttcga acaacgccaa ccgcaagggc aagcgcaagc
     1741 cggtaaggca gcaaatcctg aaggttgcaa gggaagggcg ggatttccgc gtcgtcgctt
     1801 cggccgtgct ttttgatgcc gtgatgcaac ttgggcctga acaggttgcg gcccttggca
     1861 tcgtcaagga cggatccggg ctggacgtcc tgaatatcga gggcgcgtcg tgggatcttg
     1921 caggttcaga accggccatg ctgatcggtc tgaagcagcc ggtcgggccg gaaggttcaa
     1981 taatctggcg cctgaagaat cccgatttat tccttgcttc gggcaagctt gcaccgggcc
     2041 agctttcggt ttttgcaaag gtcgatcttg gccgcgtcag ggaaaggccg gcttctattt
     2101 ccgatcttct ggttacggca gacggcatct acgtgctttc gacggtgccg gacgcacccg
     2161 atccggatca gatcggccgc cttctgcggt tgcagccggt tcagggcggg tatgtcgcca
     2221 gcgttctgtt cgagtatcgc ggactgaagc cggaaggcct gtgcgtgacg gaaggcgatt
     2281 cgttcaccgt cgtgatggac accggtgacg ccccggcgct tttccatact tccaggtggt
     2341 cacgatgatt tggcgtggtg cggcggtcat tcttgtcgtg ctgctggtgc ctccgttgac
     2401 ggcagtggcc ctggaatcct ttggggacga cgccaaaggc tcgcccgccc ctggcgccaa
     2461 tttcgatccg gcttccgatg atgggttttc cgggcttgtc atgtcggaag gcgacgtcgt
     2521 ccaggccgca gtcgattcaa gcccggaact ccagcgcctt cgccagaggg ccagtgcccg
     2581 caaggtcggc gccgacttcg cgaagtacga cgtccgcaac ccggaactgc ggatccagga
     2641 cctttcgacc gaatacttcg attcagacgc gaaccatcgt ttccagatcg gtttgcgatg
     2701 gcatcttccg cggttcggcg aagtcggcga gaacgtagcg cttgccaggt gggagtacca
     2761 ggatgcgtgc gtcgatgaat tcgaataccg ggtgcaggtg atcggccagg ccaggcaggc
     2821 ctttttcaat gtcctgctgt tgaaggacga agtcgccgcc gcgcagcggc gggaagccct
     2881 ggaggcccgt cgggttgatg tgctctcaag gcttgcggat cttggaacgg tgccgttcgt
     2941 tcgcagcatg aaggcgcaga cgtccctttt gaaggcaagg cgcgacgtgg caagcctgca
     3001 gaaggcactc ggcgccgcca ggcaggccct ggccgccatt gtcggaaacg attccccggt
     3061 cgatgcggcc gggtttgaac tgccggaagg cgagccgcaa cttgacgcaa tcatgaaaag
     3121 ggccgagtcg gttcgtcccg aactggcact ggaggcgcgc cggaccgaac tggcgcgggc
     3181 ctcccgcagg gccgaggtgc tggacatgat accgaccttc aggttcgtcc aggccgcata
     3241 tcattacgaa agccgcgaca gggactgggg cgaactgtcc ttcggaattg aaatccctgt
     3301 ctttaactgg gggcgccaca agctgcgacg ggccgaaatg gccctggcca gccgggaaga
     3361 caacggttcc gccgggatcg accaggtcag gaagcggatt ggccaggatt tcgcgcaggc
     3421 gtcggccgcc agggccgcat gggtgacctt gaaggatcat gtcgcgaagg ccagggaaga
     3481 tgtcgagcgg atggcggccg tggccaggtc gaaggggata tcggaagaag aggtcatcga
     3541 cctggagctt tccggcgccg aactggaatg gatgatggtc gatgccaggc gggattacgt
     3601 tgaagccctg cttgaccttt gcgaggcggc cggtttcgat aatttcgggg aactccttgg
     3661 cgagtgaggt ttgcttccgg tggaaggcaa caggaccgag gtcatcagca ggtacgaatc
     3721 gaagtacctt gttccgtcgt cggtcgtccc cgcgatgcgc gattacatca gcgccttctg
     3781 ttcgcccgac aggcatgtcg atcccgacgg gcgctatgtc gtcaacaacc tgtacttcga
     3841 aaccccggat cttcggttct acaacgacac ccggttgaaa aggttcacca ggttcaagcc
     3901 gaggtgccgc ttctacggcg atggctcgga cgggttcctg tggcttgaac tgaagcacaa
     3961 ggtggcaaac gtcacatgga agattcgccg gcgcataccg gtcggcggct gggacgacct
     4021 gtttatggcg aaccggttcg agcctggaag ggttgagccg ggattgaacg gggcgcgaac
     4081 ggcggccagg cgggtcaagg tcgtcgaatc gttcgaggat gctgtcatca cgttcggcgc
     4141 ggcaccgttt cttttcgtga aatatcagcg cgagccttac gtcagcgacg tcaatgcgta
     4201 tgccaggatc acgtttgatc gccagctttc gtactgcatg gccgaaggtt cctcgaaact
     4261 ggatcacggc cggcagtaca tctattatga tgacggggcg acggcgctgc atgtcgaaga
     4321 tgagtcgccc gttcttgtgg aaatcaagac cgaaaccagc gttccgacat gggtgatggg
     4381 gatgatcggc agattcgacc tgtcccgacg cggtttttcg aagtactgcc aggcggttga
     4441 ccacttcatg tgctttaaca ggccccaggc caggaatccg gccatcgacc tttccaggag
     4501 gctctttcga tgaagaatgc agtatcggtt gcctttttcc tgtcgttgct ggtcgcattc
     4561 gtcggttgcg gcggtgattc gaacccggtc ggggatgtaa cggacgacgt ggttccggtc
     4621 gaaagcgagg ttcgactgaa cgaaatcgac tgccacggaa ccgactggat tgaaatcgtg
     4681 aatgtcggtg acatggttgc ggatcttgcc ggatggattg tgaccgacgg actggacaag
     4741 accgaccatt actacacgct gcccgccggt tcaatcgtca ggcccggcgg atacctggtg
     4801 gtcaagggtg tcagcgaaac cagggaagga ttcgattttg gcatcaagtg cgacgacgag
     4861 tctgtcttcc tgatggatcc ttcgaagaag gtgatcgacg tcaccgatgt gaccgcggtg
     4921 ccggatggat tcacgtgggg acgcagcccg gacggaaccg gcgccttcac cgtcacgctg
     4981 ccgacccagg gaacggcgaa tattgaaaat cccgacacgc cggacgagct gtttgatccg
     5041 ggcgtcgtga acctgttcga aatcaccctc ggttccgatg cggtgtccgg cctgaccacc
     5101 gatccgtatg agtacgtttc cggtgtcgtc agcgtgacgg tcgccggaaa gacggtcagc
     5161 ggccagccgg tcggcgtccg cctcaagagc gggtattcgt tccagcccct gaccgccaag
     5221 gccgccttca agattaaggc cgacggttac gcggtcggcg atcgcgtgct tggaatgaag
     5281 aacttcacgc tgaacaacat ggttgacgac cgcacgatga tgcacgaaac gctggcgtac
     5341 aagcttttcc gcgaagccgg gatccccgcg ccccgcaccg gttacgccca ggttcgcgta
     5401 aacggccagg actacgggct gtacgtgctg gtggaaaatt acgacgacca gttcacgacg
     5461 ttcaattttg catccaccca gcacctgtac gagggcaccg gcgaccttta tgccggtcag
     5521 atcggcaagt tcgacgttga ggacggtgac aagctggacg ttgcggatct ggaaaagttg
     5581 atggagcgta tcgct
//
LOCUS       DAOPPA010000183         5578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_15934_length_5578_cov_3.742350, whole genome shotgun sequence.
ACCESSION   DAOPPA010000183 DAOPPA010000000
VERSION     DAOPPA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15934_length_5578_cov_3.742350"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKH54_06795"
     CDS             complement(<1..637)
                     /locus_tag="PKH54_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99633.1"
                     /translation="MRRVPFGSMLAAMILAIPAITGCGGTVDPGQDLVQPEDVSGNDV
                     ISPEDVEDIFDAGRDAAVPDVGTDQGQETGGPDIALPDCPSGQACDDGDSCTINDQCQ
                     SDGETCVGTVVESCSDNLACTYDSCVSETECTNELKPGWCLVEGECIEDGEFSAAQPC
                     MSCITALGTDRLFPDDAGVCDDGNACTTGDACHAGACVGVAMDCDDGNPCTT"
     gene            complement(680..>1730)
                     /locus_tag="PKH54_06800"
     CDS             complement(680..>1730)
                     /locus_tag="PKH54_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99634.1"
                     /translation="PLATISNPDVVVSGLDVDVDGIWGFLFNWIIDFFEDNFADMIED
                     EFETQLAGQLSELVRGAVADLALDPEFPVPELLPGMTPVTLQMKSKFSTISITPEGSD
                     IGLAAVVLPDAAQTPPAVLLGSIGRGACLGTDPGIPAFPHTYEMGLMAKDDFLNEALY
                     AIWASNGLKIPVGPEMLGDFDLSEFGISNLSLNVDFQLPPLISDCNLEHKMFFEAGDI
                     RVNAGMKLLGSQVDMVMYASLIAEARIVLVPGATGTEVGIQIETPLFIDIEVAQIAGG
                     LVGAEDTISSLIRTVALPMVLDLLSGDTLASFQLPSIDLSSLAEGFPAGSVIEIDMKE
                     VNRSRGATIVLGNVK"
     assembly_gap    1731..1740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1741..3899)
                     /locus_tag="PKH54_06805"
     CDS             complement(<1741..3899)
                     /locus_tag="PKH54_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99635.1"
                     /translation="MRTGTAFVIAVIFALLSGCGGSNPASDNGLPEDTASDYIIGSDL
                     NGQDVRLDTISPDGTGLDGEESDSSRDQGLPPEPCRPGESCDDHDPCTIDDLCDDDEE
                     CAGTPVEDCDDGIECTFDECTTETDCSHELKPGWCFVAGKCIEDGQSDPAVICRSCQT
                     ALKTDELLPDDNMTCDDGDACTVNDRCHEGFCIADARDCGDRNSCTADMCVNGSCVNE
                     AVEGMCNDEDLCTENDLCVDGQCVGSPIGCWDQNPCTQDACHPDFGCVHEFLDIPCDD
                     GNICSVGDRCWLGNCLSGDGMKDCDDGNQCTDDGCVPVREGGCVHIPNTAPCDDGDPC
                     TLEDKCKASSCDPGKGVLDCDDGNPCTTDRCEKGTGCVHDNNTVPCDDEDPCTLDDVC
                     GGGVCNPGVTQLACDDGNECTSDFCEAGIGCVYENNLNICDDDNVCTNVSTCMDGVCI
                     GESAVDCGDDDSCTLDYCLPTGDAPGCYHKPLPECRPQIVIDYPPRASTLSKKTGDQI
                     VVRGHIEYPPNAIQEDGYPSPEMAMSKVVVNGLDVWVDPRTKPWSFQSTMTLQQGMNP
                     IVAYGQANAVPAYKDYVVQSLYYSDSWFPVSHANPAAGMVSDGVKIFLGKTVWDDNNT
                     ATADDIATLLTNILKTLSLSTLIKNPVTKGEFGWCDYKVNLKNMKYGTPSIDLIPDWG
                     KIKVKVVIPRFSVDIDVPISGFACPDFSGDASATSIT"
     gene            complement(4045..>5193)
                     /locus_tag="PKH54_06810"
     CDS             complement(4045..>5193)
                     /locus_tag="PKH54_06810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOC99636.1"
                     /translation="IVTAVRTAVGKAKKGTLRNVRPETLIGTVVKAAVERAAGLDPHT
                     IDDVIVGCAMPEGPQGMNIGRIAVFAAGLPETVTGMTVNRFCSSGLQTIAIGAQAISA
                     GVADVIVAGGVESMSSVPMGGFNLMPDPDLMKNMPQVYSSMGITAENLVDKYQISRQR
                     QDEFSVASHQKAGVALADGRMKEQIVPLLVNNFDGKDPVVFDVDEGPRPGSTVEGLGA
                     LRASFKTGGSVTPGNSSQVSDGAAAVLLMSEDALKKSGLTPMARFVDFRVAGCGPEIM
                     GIGPAYAIPKLFAATGKSDADIGFYELNEAFAAQSLAVLDQLKGVVDPAKVNPNGGAI
                     AFGHPLGCTGAKLAVQSVYEMKRIGAKYSIVSMCIGGGMGAAGLLENL"
     assembly_gap    5194..5203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5204..>5578)
                     /locus_tag="PKH54_06815"
     CDS             complement(<5204..>5578)
                     /locus_tag="PKH54_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOC99637.1"
                     /translation="YDQITISRDSLLHDAKQTALGMARAGYTPPAAPDWLIMPGRDGI
                     AYLEAGLAQFLAGGMMTQHDYVVSSKLANVLCGGDVRPGTRVDEQHLLDLECEAFLSL
                     CGEEKTQDRIQYMLENGKPLRN"
BASE COUNT         1111 a         1733 c         1605 g         1109 t
ORIGIN      
        1 ccgtcgtgca cggattgccg tcgtcgcagt ccatggcgac cccgacacag gcgcccgcat
       61 gacatgcgtc gccggtcgtg caggcgttcc cgtcgtcgca gacgccggcg tcgtccggga
      121 aaagacgatc ggttcccagg gcggtgatgc aggacatgca gggctgtgcc gccgagaact
      181 cgccatcctc gatgcattcg ccttcaacca ggcaccatcc cggcttcagc tcgttggtgc
      241 actcggtttc ggaaacacac gaatcataag tgcaggccag gttgtcgctg caagattcca
      301 cgacggttcc gacgcacgtc tcgccgtcgg actggcactg atcgttgatg gtgcacgaat
      361 cgccgtcatc gcacgcctgg ccggaaggac aatcgggcag cgcgatatcg ggcccacccg
      421 tctcctggcc ctgatccgtt ccgacatccg gcacggccgc gtcacgccct gcatcgaaaa
      481 tgtcttccac atcctcgggc gaaatgacgt cgttcccgga cacgtcttcc ggctgaacca
      541 gatcctgtcc gggatcgacg gttccgccac aacccgtgat tgccggaatc gcaaggatca
      601 tcgcggccag cattgaacca aacggcactc ttctcatcgg tcgtttctcc attcggcagg
      661 acttcggacg tcgaagccgt tacttcacat tgccaagaac tatggtcgct cccctggaac
      721 gattgacttc cttcatgtcg atttcgatca ccgacccggc cgggaatccc tcggccagcg
      781 agctcaagtc gatgcttgga agctggaacg aagccagcgt gtccccggac agaagatcaa
      841 gaaccatcgg aagggcgacc gtccggatca gcgaactgat cgtgtcttcg gcaccgacaa
      901 gccccccggc aatctgggcg acttcaatat cgatgaaaag cggcgtctcg atctgaatgc
      961 cgacctctgt tccggtcgcg cccggcacca ggacaatgcg ggcctcggcg atcaggctgg
     1021 cgtacatcac catgtcaacc tgggagccga gcagcttcat gcccgcgttc accctgatat
     1081 ccccggcctc gaagaacatc ttgtgttcca gattgcagtc cgatatcaac ggcggcaact
     1141 ggaaatcgac attcaggctg agattggaaa tcccgaactc ggaaaggtca aaatcgccga
     1201 gcatttcggg cccgaccgga atcttcagtc cattcgacgc ccagattgcg taaagggcct
     1261 cgttaaggaa atcatccttg gccatcagcc ccatttcgta tgtgtggggg aacgcgggaa
     1321 tcccgggatc ggttcccagg caggcgccgc gaccgataga accgagcagc accgccggcg
     1381 gcgtctgggc ggcatccggc aggacgaccg cggcaagacc gatatcgctg ccttcgggcg
     1441 tgatggatat cgtggaaaac ttgctcttca tctggagcgt gaccggcgtc attcccggca
     1501 gaagttcggg aaccgggaat tccgggtcaa gggccagatc ggccacggcg cccctgacca
     1561 gttcggaaag ctggccggct agctgggtct cgaactcgtc ctcgatcatg tcggcaaagt
     1621 tgtcttcgaa gaagtcgatg atccagttga aaaggaagcc ccagatgcca tcgacatcga
     1681 catcaagccc ggaaaccacg acatccggat tggaaatggt ggcaagcggc nnnnnnnnnn
     1741 atcgttatcg aagtcgccga ggcatccccg ctgaaatcgg gacacgcgaa accgcttatc
     1801 gggacgtcga tatccacgct gaacctgggg atcacgacct tgacctttat ctttccccag
     1861 tcgggaatca ggtcaatcga cggcgtcccg tatttcatgt tcttcaggtt gaccttgtaa
     1921 tcgcaccagc cgaactcgcc cttggtgacc ggattcttga taagcgtgga aaggctgagt
     1981 gttttcagga tgttggtcaa cagggtcgcg atgtcgtcgg cggtcgccgt gttgttgtcg
     2041 tcccagaccg tcttgcccag gaatatcttc acgccgtcgc tgaccatccc ggcggccgga
     2101 ttggcgtgac tgaccgggaa ccacgagtcc gagtagtaaa gagactggac gacgtaatcc
     2161 ttgtatgccg gcaccgcatt ggcctggccg tacgcaacaa tgggattcat cccctgctgc
     2221 agcgtcatcg tgctctggaa tgaccacggc ttcgtcctgg gatcgaccca gacgtcaagg
     2281 ccgttgacga cgaccttgct catggccatt tccgggctcg gataaccgtc ttcctggatt
     2341 gcgttcggcg gatactcgat atgcccgcga accacaatct ggtcgccggt cttcttgctc
     2401 agagtggatg cgcggggtgg atagtcgatg acaatctgcg gcctgcattc gggaagcggc
     2461 ttgtgatagc agccgggcgc gtcaccggtc ggcaggcagt aatcaagggt gcaggaatca
     2521 tcatcgccgc aatcgaccgc ggactcgccg atgcacacgc cgtccatgca cgtcgatacg
     2581 ttcgtgcaca cgttgtcgtc gtcgcagata ttcaggttgt tttcatacac gcagccaatc
     2641 ccggcctcac agaagtcgct ggtgcactcg ttgccgtcat cgcaggcaag ctgcgtcacg
     2701 ccgggattgc agacgccacc gccgcagacg tcgtcaaggg tgcacggatc ctcgtcatcg
     2761 caaggcacgg tgttgttgtc atgaacacat ccggtaccct tctcgcacct gtccgtcgtg
     2821 cagggattcc catcgtcgca gtcaaggacg cccttgcctg gatcgcacga gctggccttg
     2881 cacttgtctt ccagcgtgca cgggtcgcca tcgtcgcacg gcgcggtatt cgggatgtgg
     2941 acgcaaccgc cttcacgaac cggcacgcag ccgtcgtcgg tgcactgatt gccgtcgtcg
     3001 caatccttca ttccgtcgcc ggaaaggcaa ttgccaagcc agcagcgatc cccgacgctg
     3061 cagatgttcc cgtcgtcgca cggtatgtcc aggaactcgt ggacacatcc gaaatcggga
     3121 tgacaggcat cctgggtgca gggattctga tcccagcaac cgatgggtga accgacgcac
     3181 tgaccatcga cacagaggtc gttttcagta caaaggtctt cgtcgttgca catgccttcg
     3241 acggcctcgt tgacgcagga accgttaacg cacatgtcgg ccgtgcatga gttgcgatcg
     3301 ccgcagtcgc gggcgtcggc aatgcagaaa ccttcatgac acctgtcgtt gacggtgcag
     3361 gcatcgccgt cgtcacaggt catgttgtcg tcgggaagga gttcatcggt cttcagtgcc
     3421 gtctggcagg atctgcaaat cacggcagga tcggactgac cgtcctcgat gcacttgccc
     3481 gccacgaaac accatcccgg cttcaattca tgcgaacagt cggtttcggt cgtgcattcg
     3541 tcgaaagtgc attcgattcc gtcgtcgcag tcctcgaccg gcgtcccggc gcactcctcg
     3601 tcgtcgtcgc acaggtcgtc gatcgtgcac ggatcatggt cgtcgcacga ttcgccgggc
     3661 cgacaaggtt ccggcggcag accctgatcc cgtgaagaat cagattcttc cccgtccaga
     3721 ccggtgccgt cgggcgaaat cgtgtccagg cggacatcct gtccgttcag atccgacccg
     3781 atgatgtaat ccgatgcggt atcttccgga agtccgttgt cggacgcggg gttcgagccg
     3841 ccgcaaccgg aaagcaacgc gaaaatgact gcaatgacaa atgcggttcc agttctcacc
     3901 agaaacctcc tgaattttca agatcgggca atcgcgaccg cgccgaaatc gggaaggacc
     3961 agaatggccg cgcccgcagg acggatcaca tcgtgcgaat tttagggaaa aatcagagct
     4021 taaacaatag atgaatgcag gggatcagag gttttcaaga agtccggccg cgcccattcc
     4081 gccaccgatg cacatcgaca cgatgctgta cttcgccccg atccgcttca tttcataaac
     4141 cgactgaacc gcgagcttgg cgccggtgca tcccagcggg tgaccaaagg caatggcacc
     4201 gccgttcgga ttcaccttgg caggatcgac aacgcccttg agctggtcga gcacggccag
     4261 cgactgggcc gcgaacgcct cgttcagttc atagaaaccg atgtcggcat ccgacttgcc
     4321 ggtggccgcg aacagcttcg gaatggcgta cgccggacca atgcccatga tttccgggcc
     4381 acagccggcg accctgaaat cgacaaagcg ggccatcggc gtcaggccgg acttcttcag
     4441 agcgtcctcg ctcatcagaa gaaccgccgc ggcgccgtcg ctgacctgtg acgagttgcc
     4501 gggggtcacg ctgccgcctg tcttgaacga tgcccggaga gccccgagtc cctcgaccgt
     4561 cgaacctggc cgtggtcctt catcgacatc gaagaccaca gggtcctttc catcgaagtt
     4621 gttaaccagc agcgggacaa tctgttcctt catgcggccg tcggcaagcg ccacgcctgc
     4681 cttctggtgc gaagcgacgg aaaattcgtc ctggcgctgg cgactgatct gatacttgtc
     4741 caccagattt tcggccgtga tccccatcga ggaatatacc tgcggcatgt tcttcatcag
     4801 atccggatcg ggcatcaggt tgaatccgcc catcggaacc gaggacatgc tctcgacgcc
     4861 accggccacg atgacatcgg caacgccggc ggaaatcgcc tgggcaccga ttgcgatcgt
     4921 ctgaagaccg gaagagcaga aacggttgac agtcatgccg gtgacggtct cgggcagccc
     4981 ggccgcaaac accgcgatgc ggccgatgtt cattccctgc ggaccttccg gcatcgcgca
     5041 tccgacaatc acgtcgtcga tggtgtgggg atcaagcccc gccgcccttt cgaccgcggc
     5101 cttcacgacc gtgccgatca gggtttcggg cctgacgttg cgcagggttc ccttcttcgc
     5161 cttgccaaca gccgtcctga cggcagtcac gatnnnnnnn nnntagtttc tcaggggttt
     5221 tccgttttcc agcatgtact ggatacggtc ctgggttttt tcctcgccgc acagcgacag
     5281 gaatgcctcg cattcgagat cgagcaggtg ctgctcatcg acccttgtgc ccggccttac
     5341 atcgccgccg caaaggacgt tggcaagctt gctgctgacc acgtagtcgt gctgcgtcat
     5401 cattccgccg gccaggaact gggccagtcc ggcttcaagg tacgcgatgc cgtcgcgacc
     5461 gggcatgatc agccagtcag gcgccgcagg cggcgtatag ccggcacggg ccatgccaag
     5521 cgcggtctgc ttcgcgtcgt gcagcaatga atccctggaa atggttatct ggtcgtac
//
LOCUS       DAOPPA010000184         5541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16077_length_5541_cov_3.776340, whole genome shotgun sequence.
ACCESSION   DAOPPA010000184 DAOPPA010000000
VERSION     DAOPPA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16077_length_5541_cov_3.776340"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKH54_06820"
     CDS             complement(<1..1129)
                     /locus_tag="PKH54_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC99638.1"
                     /translation="MKRTTTRLRNGHGFGRAGRMLAITLLMAAAAGCFDASRAEDVPG
                     LQAVVQDWRDEIVYQVLVDRFANGDRNNDFNVVPHSPAAYHGGDWQGLIDRLDYLDEL
                     GVTALWISPVVKNVEEDAGVAGYHGYWTQDFTQPNLHFGDLAKLRELVGKAHERGMKV
                     ILDIVTNHVGQLFYYDINRNGQPDELVMGSGTASPITRTTEWDPDFDEDGIQAFTSLG
                     YSGPAPINWVWQPEINRVPPWPPEFANDEWYNRMGRVTVWGREKQACFDAGRITAEQR
                     DSGEWWGAMPACWDYVRMQEVLGDFPGGLKDVRTELPEVRDAMIDVYAAWVERVGFDG
                     FRIDTIKHVEHEFWADFCPGIRQRAAAAGKDNFLMFGEAFDG"
     gene            complement(1173..2747)
                     /locus_tag="PKH54_06825"
     CDS             complement(1173..2747)
                     /locus_tag="PKH54_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOC99639.1"
                     /translation="MSLFNRWIATVCVLAVMAMPTMAARAGQAGPADGATESDGDVPP
                     AVVPEDSGSDESVRRKKGDGASPDDEVKPPKKPGAADGFPKGFQFGTYGRVGFSSDFA
                     GAPGNATNIVTHGSRLMEGSYVELDLGYGIRTASGFAMRLLITPAFLDEFFHFDGKAS
                     DSIVLRNLYVEASGIFKTDLKIWVGSRMYRGDDIYLLDWWPLDSLNTLGGGLSWDHKS
                     WQVLAHVGVNRLDDEYQLRRFQVPATYGSDTITLMERQRMIASAKVSWLGRDLAPKLS
                     MKVSLYGEFHWLPKGKLRYDSEFLDYFKEFGIAPDPELVSEMPADNGGVVGAQIGFWG
                     FGKDSHVNIFARYAWGLAAYGEWGIPWGVALDRTASGAKEFVLAASSNWENRHFGIMA
                     AAYGRLFVDADSNRYDLDDYWEGTEDVRATWFATDHFHVGAELSHQWKASRGLDPTDD
                     KVGRPHVVQMSLMPSLNLERGMYQRPQIRLVYTASWSNDDARSLLPQFDERRGQEWQH
                     YLGVQVEWWLNSSSYQ"
     gene            complement(2744..3295)
                     /locus_tag="PKH54_06830"
     CDS             complement(2744..3295)
                     /locus_tag="PKH54_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99640.1"
                     /translation="MNTRSRLARSGIRTAIAACLAAAVPFVFAGCGEGGSEINDVAGA
                     DDSGTGTDIVEWDPGSGGTGCGGYGYGYGPDCVPEGALDCTQDPCIFGTCELVAGVGT
                     CVCLEGYDGRICGECAQGWVVRGLRCVRVDACHGFPCAFGTCRIVNTQPFCDCDTGYA
                     GDHCDACADGFIPKNLRCVLEEE"
     gene            complement(3292..5487)
                     /locus_tag="PKH54_06835"
     CDS             complement(3292..5487)
                     /locus_tag="PKH54_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC99641.1"
                     /translation="MAWNCPKGSGNRTIHGVAAGHGVAAACALVLLAAVDSGCGNGAP
                     DPGLPVRTCEVGFEWKAAAAEVAVVGEFNDWRPAGYELARDPDGVFRGSFVVGPGRHA
                     YRFLVDGEERMDPSNPLTMFSRDGFEHSALVQSDCNRAAFKVESAQVSGDRFKARLVC
                     MAGAGGLACDPDSVAVTLDGMAVSAVMKTGSLDVDLGPLDAGKHRLTVAATDTGGTPA
                     EPLVMPFWVEAEPFDWSDALVYQVVVDRFRRGDGPLDADAGITMRMGGDWAGVVAAIE
                     EGFFERLGVNALWISPAVRNAEGSWPCFDGRTYESYHGYWPVATRAAEPHFGGDAGLA
                     ALVDAAHDRGIRVILDIVPNHVHIDHPWFTDHPGWFNHPEGDCVCGRQCSWTADIDHC
                     WFTGYLPDLDWRNREVLETMLSDVGWWLSNFDVDGLRIDAVPMMPRLVTRHLRDTVTR
                     TFAPGAPHVHMVGETFTGSAGRDQIRWYLGPYGLSGQFDFPVMWTLRSVVGQGYGTME
                     SLLDEVDASTAAWAGSGAVMSQFIGNHDVPRFVTVAAADDTDPLNPAPQPDVAEPYRR
                     AAVALAFVLTQPGAPVIYQGDEIGMAGGGDPDNRRPMPDPATWLAHQRWLFGQVSMLG
                     RLRAGIDAFRHGSRTDLARAADHVAFRMTGSRDTAVVALNRGSAPLRLTIPAGSWTAD
                     DAPADCLGGTVTMRNRELELIVPALGAMVVLPRDRCPAPDDYADLEVTE"
BASE COUNT         1042 a         1931 c         1622 g          946 t
ORIGIN      
        1 tgccgtcaaa ggcctctcca aacatcagga aattgtcctt gccggccgcc gccgcccgct
       61 gacggattcc cggacagaaa tctgcccaga attcgtgctc gacatgcttg atcgtgtcga
      121 tccggaatcc gtcgaatccg accctttcaa cccacgccgc ataaacgtcg atcatcgcgt
      181 cgcgaacctc gggcagttcg gtgcgcacgt ccttcaggcc gccgggaaaa tcgcccagca
      241 cttcctgcat ccgcacgtag tcccagcacg caggcattgc cccccaccat tcccctgaat
      301 cccgctgttc ggcggtgatg cgtcccgcgt cgaagcaggc ctgcttttcc cggccccaga
      361 ccgtcacgcg ccccatgcgg ttgtaccact cgtcgttcgc gaactccggc ggccacggcg
      421 gcacgcggtt gatttccggc tgccacaccc agttgatcgg ggccggtccc gagtagccaa
      481 gcgaagtgaa ggcctggatg ccgtcttcat cgaagtccgg atcccattcg gtcgtcctgg
      541 ttatcggcga tgccgtgccg gatcccatca ccagttcgtc cggctggccg ttgcggttga
      601 tgtcgtaata gaaaagctgg ccaacgtgat tcgtgacgat gtccaggatg accttcatcc
      661 cgcgttcatg ggccttgccg acaagctcgc gcagcttcgc cagatcgccg aagtgcaggt
      721 tgggctgggt gaagtcctgg gtccagtaac cgtgataccc ggcaaccccg gcgtcttcct
      781 cgacattctt gacgaccggc gaaatccaca gtgccgtgac gccaagttcg tccaggtagt
      841 ccagccggtc gatcaacccc tgccagtcac ctccgtgata ggcggccggc gaatgcggta
      901 cgacgttgaa atcgttgttc cggtcgccgt tggcgaaacg atcgaccagc acctgataca
      961 cgatttcatc gcgccagtcc tggaccaccg cttgcagccc gggaacatcc tcggcccgcg
     1021 atgcgtcgaa gcacccggcc gccgccgcca tcagtaacgt tattgcaagc atgcgccctg
     1081 cgcggccgaa gccatgtcca tttcgcaggc gcgtcgttgt ccgcttcatg tcaaaacctc
     1141 cgatccagca atccttccgg cattcccgtc ggtcactgat aactggaact gttcagccac
     1201 cactcgacct ggacacccag gtaatgctgc cattcctggc cgcgccgctc gtcgaactgc
     1261 ggcaacaggg atcgtgcgtc gtcatttgac cagcttgccg tatagaccag tcgaatctgc
     1321 ggcctctggt acatgccgcg ttccaggttc agggacggca tcagggacat ctggacgacg
     1381 tgcggccggc cgaccttgtc gtccgtcgga tcaaggcccc tggatgcctt ccactgatgg
     1441 ctcagttcgg ccccgacgtg gaagtgatcc gtggcgaacc acgtggcccg cacgtcctcg
     1501 gtaccttccc agtaatcgtc caggtcatac cggttggaat ccgcatcgac gaacaggcgc
     1561 ccataagccg cggccatgat cccgaaatgc cggttttccc agttcgatga agccgcaagg
     1621 acgaattcct tcgcgcccga ggcggtgcga tccagtgcga ccccccacgg aattccccac
     1681 tcgccgtatg cggcaaggcc ccatgcgtac ctggcaaaga tattgacgtg ggaatctttc
     1741 ccgaagcccc agaaaccgat ctgggcgccg accacgccac cgttgtcggc cggcatctcg
     1801 gacaccagtt ccgggtcggg cgcaatcccg aattccttga aataatccag gaattcactg
     1861 tcgtaacgca gcttgccctt tggaagccag tggaactccc cgtaaagcga aaccttcatc
     1921 gacagtttcg gggccaggtc acggccaagc cagcttacct tggccgacgc gatcatccgc
     1981 tggcgctcca tcagggtgat ggtatccgag ccataggtgg ccgggacctg gaaacggcgc
     2041 aactggtact cgtcgtcaag gcggttgaca ccgacatgtg cgagtacctg ccaggacttg
     2101 tgatcccacg acaggccgcc gccaagtgtg ttcagggaat caagcggcca ccagtcaaga
     2161 agatagatgt cgtcgcccct gtacatgcgg gatccgaccc atatcttcaa atcggtcttg
     2221 aagattcccg acgcctcgac gtaaaggttc ctcaagacga tggaatcgct tgcctttccg
     2281 tcgaagtgaa agaactcgtc caggaacgcc ggggtaatca gcagccgcat ggcgaacccg
     2341 ctggcggtgc gtattccata acccaggtcc agttcgacgt atgagccttc catcaggcgt
     2401 gaaccgtgcg tgacgatatt ggtcgcgttg ccaggggcac cggcgaaatc gctggaaaag
     2461 ccgacacgcc cgtacgtccc gaactggaat cctttcggga agccatcggc ggcccctggc
     2521 ttcttcggcg gcttgacctc gtcgtccggc gatgcaccgt cacccttctt cctgcggacg
     2581 gactcatcgg aaccggaatc ttcgggcacc accgcgggcg ggacatcgcc atccgattcg
     2641 gtggcgccgt ccgccgggcc tgcctggccg gcacgggcgg ccatggtcgg catcgccatc
     2701 accgccagca cgcacaccgt ggcaatccat cggttgaaca agctcattct tcctccagca
     2761 cgcagcgcag gttcttcggg atgaacccgt cggcgcaggc gtcgcagtga tccccggcat
     2821 aaccggtgtc gcagtcgcag aacggctgcg tgttgacgat gcggcatgtt ccgaatgcac
     2881 acgggaatcc gtggcacgca tcgacgcgca cgcaccgaag cccccggacg acccatccct
     2941 gggcgcattc gccgcagatg cggccgtcgt aaccttccag gcagacgcag gttccgaccc
     3001 cggcaacaag ttcgcatgtg ccaaaaatgc acggatcctg cgtgcagtcc agggcgccct
     3061 ccggaacgca gtccgggccg tacccgtaac cgtaaccgcc gcaaccggtt ccgccgctgc
     3121 ccggatccca ttcgacgatg tccgttccgg tgccggagtc atccgccccg gcgacatcgt
     3181 tgatttcgga ccccccttcg ccgcagcccg cgaagacaaa cggcactgcc gccgcaaggc
     3241 acgcggcgat cgccgtccgt atcccggacc tggccagcct tgagcgcgta ttcattccgt
     3301 tacctccaga tccgcataat cgtccggggc cgggcaccgg tcgcgcggaa gcacgaccat
     3361 cgccccaagc gcgggcacga tcaattccag ttcacggttc ctcattgtca ccgtaccgcc
     3421 caggcagtcg gccggggcat cgtcagccgt ccaggatccg gcgggaatag tcaggcgcaa
     3481 cggcgccgag ccacggttca aagcaaccac ggcggtgtcc ctggaaccgg tcatcctgaa
     3541 tgccacgtga tcggcggcac gggcaagatc ggtgcgcgaa ccgtgccgga acgcgtcgat
     3601 tcccgcgcgc agccgaccaa gcatggaaac ctgcccgaaa agccacctct ggtgcgccag
     3661 ccatgtcgca gggtccggca tcgggcgacg gttgtcggga tccccgccgc ccgccatgcc
     3721 gatttcgtcg ccctggtaaa tcaccggcgc gcccggctgc gtcaggacga acgccagcgc
     3781 gacggcggcg cggcggtatg gctccgcaac atccggctgc ggcgccggat tcagcggatc
     3841 agtgtcgtca gccgcggcga ccgtcacaaa gcgcgggacg tcgtggttgc ctatgaactg
     3901 gctcataacc gcgcccgacc cggcccaggc cgccgtcgag gcatccactt catcaagaag
     3961 cgactccatc gtgccgtatc cctgcccaac cacggaacgc agcgtccaca tcaccggaaa
     4021 atcgaactgt ccgctgagcc cgtacggccc aaggtaccag cgaatctggt cgcggccggc
     4081 cgaaccggtg aaggtctcgc cgaccatgtg gacatggggc gcgcccggcg cgaaagtgcg
     4141 cgtcacggtg tcccgcaggt gcctggtgac cagcctgggc atcatcggca ccgcgtcgat
     4201 gcgcaaacca tcgacgtcga aattcgacag ccaccagccg acgtccgaaa gcatggtttc
     4261 aagcacttcc ctgtttcgcc agtccaggtc gggcagatac ccggtgaacc agcagtgatc
     4321 gatgtcggcc gtccacgagc actggcggcc gcacacgcaa tccccttccg gatggttgaa
     4381 ccatcccggg tggtcggtaa accacggatg gtcgatgtgg acgtgattcg ggacgatatc
     4441 caggatcacc ctgatgcccc tgtcgtgcgc ggcatccacc agggccgcca gtccggcgtc
     4501 cccgccgaaa tggggttcgg ccgcgcgggt cgcgaccggc cagtatccgt gataggactc
     4561 gtacgtccgg ccgtcgaagc agggccagga gccttcggca ttccgtaccg ccggcgatat
     4621 ccacaaggcg ttgacgccca ggcgttcaaa gaagccttcc tcgatggcgg caacgacccc
     4681 ggcccagtca ccgcccatgc gcatcgtgat gccggcatcc gcgtccagcg gcccgtcgcc
     4741 acgccggaaa cgatcgacca cgacctggta aaccagcgcg tccgaccagt caaaaggctc
     4801 ggcctcgacc cagaacggca tgacaagcgg ctcggccggc gtaccaccgg tatccgtcgc
     4861 ggcgactgtc aggcggtgct tgccggcgtc caggggcccc agatccacat ccagggaacc
     4921 ggtcttcatg accgccgaga ccgccatgcc gtcaagcgtg accgccaccg aatccgggtc
     4981 acaggcaagg ccgccggcac cggccatgca gacaagccgc gccttgaagc ggtcgccgga
     5041 tacctgggcg ctttcgacct tgaaggccgc cctgttgcag tcggactgga ccagagcgct
     5101 gtgctcgaac ccgtcacggc taaacatagt caacgggttc gacgggtcca tccgctcttc
     5161 gccatcgacc aggaagcgat aggcgtgccg ccccgggcca accacgaagc tgccgcggaa
     5221 tacgccgtcg gggtcgcgcg cgagttcgta tccggctggc cgccagtcgt tgaattcacc
     5281 gaccaccgcg acctcggccg cggccgcctt ccattcgaac ccgacctcgc aggtgcggac
     5341 aggcagcccc ggatcaggcg ccccgttgcc gcagccggaa tccaccgcgg ccagcaggac
     5401 cagcgcgcag gcggccgcga cgccgtgacc ggccgcgact ccatgaatgg ttctgttgcc
     5461 acttcctttg ggacagttcc aggccacgac cgttcacctg ttcccgtcga aaaccacacc
     5521 gacgtcaccg gcaccgcgcc a
//
LOCUS       DAOPPA010000185         5507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16203_length_5507_cov_4.098496, whole genome shotgun sequence.
ACCESSION   DAOPPA010000185 DAOPPA010000000
VERSION     DAOPPA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16203_length_5507_cov_4.098496"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1141
                     /locus_tag="PKH54_06840"
     CDS             329..1141
                     /locus_tag="PKH54_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99642.1"
                     /translation="MIEFVDGLDWNHPRHDKWIINVSTQFGCPVGCRFCDAAHRYHGN
                     LSADEMLAQVRHVTDIHPGLAEKCRKLKVHFARMGEPALNRAVLDAIERLPATVSSPG
                     LWACVATTAPRGTEDWFERLIDVRDRLFRKRFQLQFSINTTDEDFRQDMTAVRLLPFK
                     WIRDYASRFFQDGERKVILNFALGRAVPFDAHEILRQFDPVTTCVKLTPLNPTARGAE
                     NGMSGINREDLERIATMLNENGFDAVLSIGDPREDDIGSNCGQSVRRLADRR"
     gene            complement(1116..3380)
                     /locus_tag="PKH54_06845"
     CDS             complement(1116..3380)
                     /locus_tag="PKH54_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99643.1"
                     /translation="MHHQSIALSGICLTLAALCGCAPDAAEPVHRQTWCVTDSAMDRQ
                     TRHRAVTGVMDLMKTTVIEDGSIVLLNRFTLTDADRNLFQIRPDGHVAKWEFSCSDPF
                     DVRKVMTFSEGGIEYVVETFSTVERSNVAQRAQMVRVTVFGDNGAGGRFHVMNKVDGE
                     PVDLLADLSARSARLGVPPENPAAFQAAKGSFIVPASAAAQDCELSGGVLECRFQVGP
                     DQSVVFDLQIVPGTEEDLPRDQQDRVTFDRDAALAGEQAVLKAFMDRLPNLSLPDQWH
                     VFAFYSSAWLINIFRDSFDDLFLVKPGAFEYNSFWNRDAAFITRTMDLLGLHERAGES
                     MRVFWRRPLPDVVNDQGTWGGTIEQKSDGRWECPDHEWDGQGQVTWALVTHYRFTRDP
                     EFLENAYPAISLAMDWMIDNIEPRPSGHNLLPSGYGETLDTWNYVLLHNYWGVLGLDE
                     AAWLAAEAGRPEDSALWGAQADQFRMWVRQAALDAYVGDSESGYIASAVGTESKRAWG
                     SIRTVFPPRIMDPLSPEVGNTFEWLWKNRAQDQYRFEDDGNRIWTYMAAEWSQALLIR
                     GEWERAWTLMNGFRAQASPIFGWWEEKYLNTGKGRGDSPHGWAAADYVSWLRSMLVRE
                     VHAPLPDADAALVDPSTGVTVELLGGVPPEWYVVGSPIVFERVPTTFGRLDAMSVQTG
                     EDGARHVVADFELHQASTGPAMDEFGVMIRMRGAVVTEAACAEGTVEFQADGVVVRGT
                     DHIDCTIIVDQPAS"
     gene            complement(3397..>5507)
                     /locus_tag="PKH54_06850"
     CDS             complement(3397..>5507)
                     /locus_tag="PKH54_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99644.1"
                     /translation="LSDFRLRSVAPFSGPGESWLVDELDRDFLAGTVLASRKASGGEF
                     FAPLVNDTRQSGDRRQMLVLPQAPKVPPAGIADMPGIMSAGVPVVPAWPMKNVVAVTI
                     ILPESDGTIDTNREDWTKTLEDQVYNEVVAGMAWWPEKAESYDVDLSFKVYIFKPSVH
                     DEVKTGYEPITHNSQDEGVWIADVLNNLGYSEGYYTASVDALNAYMKGYAEADHAITI
                     FVVNSQNDRDDMFPDGYFAYAHWGGPLIMMTTGNGGYGLSMMDTVATHEMGHSFYAVD
                     EYDSPGYAECSCNDGYNGCKNRNCMKGCGLNMPCMMRHNEDGLCTDTVCHVGWKCQCS
                     SGACCDGCGYRPGSFVCRAKAGDCDLAEFCTGGSTSCPADKFKDTSYTCRSVRGECDV
                     AEKCGGTSPQCPTDTFLKASTVCRESNGVCDVAESCTGSSAECPANAFSGKDVQCRKS
                     GGDCDPAEFCTGKGADCPSDKLSDQNHVCRESTDLCDAAETCDGKGTDCPTDKAASAS
                     TVCRKAAGGCDQEEYCDGSSMSCPADQLKRSGSVCREASGGCDREEICDGLSATCPPD
                     YIQGKGFVCREAAGGCDVAERCAGTGVDCPVDLIRGAGFVCRAAAGQCDREEKCSGTS
                     VQCPEDLFAESGTTCSDGNPETVDDQCGKDGTCQGDVPMPIAGCSGASGGSGASSGLA
                     LLLATLVLALVSFRRRRFPA"
BASE COUNT         1142 a         1768 c         1556 g         1041 t
ORIGIN      
        1 ggctcgactg cccgcccggc acgcggggaa tcctgacggc cggcgacatg aaccgaacca
       61 ccgaccgata tgtgctgcct gccatggcgg atggccgcgc ggcagcattt tcttcattta
      121 tatcaataac cagggaggca tgatgcagtt tgggcagacg tctgaccaac agacaccagt
      181 taacttcaac gatagaaagg actcggaagg ggccgtttca gaaggcaaag cgttcaatcg
      241 aaagggactg gaagtggcat ccacgtgcgg agacccttcc gttgcaacga tatttgttgc
      301 caggacccgc ccgggtgacg acacgagcat gatcgaattc gtcgatggac tggactggaa
      361 ccatccacgc catgacaagt ggatcatcaa cgtttcaaca cagttcggct gcccggtggg
      421 gtgtcgcttc tgcgatgctg cccaccgata ccacggcaac ctgtcggcgg acgagatgct
      481 ggcccaggtt cgacatgtca ccgacatcca tccgggactt gccgaaaaat gccgcaagct
      541 gaaggtacac tttgcccgga tgggcgagcc ggccctgaac cgcgccgtcc tggacgccat
      601 tgaacgactt cccgcgaccg tgagttcccc gggcttgtgg gcctgcgtgg cgacaactgc
      661 gccccggggc accgaggact ggttcgaacg cctgatcgac gtccgggaca ggctttttcg
      721 caaaagattc cagcttcaat tctcgatcaa caccacggat gaagacttcc gccaggacat
      781 gacggcggtg cgccttcttc cgttcaagtg gattcgggac tacgcgtcgc ggttcttcca
      841 ggacggcgaa agaaaggtca tcctgaactt cgcgcttgga agagcggtac cttttgacgc
      901 gcacgaaatc ctgcgtcagt tcgatccggt taccacatgc gtgaagctga ccccgctgaa
      961 tccgacggcc cgcggggcgg aaaacggaat gtcgggcatt aatcgcgaag accttgaaag
     1021 gattgccaca atgctgaacg aaaacggctt tgacgcggtg ctttcgatcg gggatccacg
     1081 cgaggatgat atcggaagca actgcggcca gtcggtcagg aggctggctg atcgacgatg
     1141 atcgtgcagt cgatgtggtc ggtgcccctg accacgacac cgtccgcctg aaattcaact
     1201 gttccttcgg cacatgcggc ttccgtgacg accgcccccc gcattcgaat catgacgccg
     1261 aattcgtcca tggccggtcc ggtcgaagcc tggtgaagtt cgaaatccgc aacgacatgc
     1321 ctggcaccgt cttcgccggt ctgaaccgac atggcatcaa gacgtccaaa cgttgtcggc
     1381 acgcgttcaa acacgatcgg ggacccgacc acataccatt ccgggggcac accgccaagc
     1441 agttcgaccg tgactccggt cgagggatcg accagggcgg cgtccgcgtc gggaagcggc
     1501 gcatgaacct cgcgcacaag catggaccga agccaggaaa cataatcggc ggccgcccat
     1561 ccatgcgggg agtcgccacg gcccttgccc gtgttcaggt acttttcctc ccaccatccg
     1621 aaaatcggcg acgcctgtgc acggaacccg ttcatcagcg tccaggcccg ttcccactcg
     1681 ccccgtatca ggagcgcctg cgaccactcg gcggccatgt atgtccagat gcggttgccg
     1741 tcgtcttcaa agcggtactg atcctgggcc cggtttttcc acaaccattc aaaggtgttg
     1801 cccacttccg ggcttaacgg atccattatt cttggtggaa agaccgtcct gatcgaaccc
     1861 caggcccgct tcgattccgt tccaaccgcg gacgcgatgt acccggattc cgaatccccc
     1921 acgtatgcat ccagcgccgc ctgccgcacc cacatcctga actggtcggc ctgggcaccc
     1981 cacagggcgg aatcctcggg cctgccggcc tcggctgcca gccacgcggc ctcgtcaagt
     2041 ccaagcacgc cccagtagtt gtgaagaagc acgtaattcc aggtgtcaag cgtctcgccg
     2101 tatccggaag gcagcaggtt gtgaccgctc ggcctgggtt cgatgttatc aatcatccag
     2161 tccatggcca gtgaaatcgc gggatacgca ttttcaagga attccggatc cctggtaaac
     2221 ctgtaatggg ttaccagtgc ccaggtcacc tggccctgcc cgtcccactc gtgatcgggg
     2281 cattcccagc ggccgtcgga cttctgctcg atcgttcccc cccacgttcc ctggtcgttt
     2341 acgacatccg gcagtggtcg ccgccagaag acacgcatgc tctccccggc cctttcatga
     2401 agccccagaa ggtccatcgt ccttgtgatg aacgccgcgt cgcggttcca gaacgagttg
     2461 tattcaaagg cgcctggctt gaccaggaag aggtcgtcga agctgtcgcg gaagatgttg
     2521 atgagccaag cggaagagta gaaggcgaac acatgccact gatccggaag cgaaagattc
     2581 ggcaaccgat ccatgaacgc cttaagcacc gcctgttcgc cggcaagcgc ggcgtccctg
     2641 tcgaaggtga cgcgatcctg ctgatcacgg ggaagatcct cctcggtgcc cggaacgatc
     2701 tgaagatcaa acaccacgga ctgatccggc ccaacctgga accggcattc aagaacaccg
     2761 cccgacagtt cgcagtcctg cgccgcggcc gatgcaggga cgatgaacga acctttggct
     2821 gcctgaaaag ccgcggggtt ttctggtgga acacccagcc gggcggatcg ggccgacaga
     2881 tcggccagca gatcgaccgg ctcgccgtca accttgttca tgacgtgaaa tcggccgccg
     2941 gcaccgttgt cgccaaaaac cgtgacccgc accatctgcg cgcgctgcgc gacattgctt
     3001 cgttcgacgg tcgaaaacgt ttcgaccaca tattcgatgc caccttccga aaacgtcatg
     3061 accttgcgga catcgaacgg atccgaacac gaaaattccc acttcgcgac atggccgtcc
     3121 ggccggatct ggaaaagatt cctgtcggcg tcggtaagcg tgaacctgtt aagaagcacg
     3181 attgaaccgt cttcgatgac ggtcgtcttc atcagatcca tcacgcccgt gacggccctg
     3241 tgcctggtct ggcgatccat ggccgaatcg gtcacgcacc aggtctgccg gtgaaccggt
     3301 tctgcggcat ccggcgcgca accgcacaag gcggccaggg tcaggcaaat ccccgacagg
     3361 gcgatcgact ggtgatgcac gggcaacctc ctgatttcag gcgggaaaac gacggcgacg
     3421 gaacgaaaca agggcaagca ccagggtcgc cagcagcagc gcaaggccgg acgacgcgcc
     3481 agacccgccg gaagccccgc tgcacccggc gatcggcatg ggaacgtcac cctggcaggt
     3541 gccgtccttt ccgcattggt catcgacggt ttccggattg ccgtcgctgc acgtcgtgcc
     3601 ggactcggca aacaggtctt cgggacactg cacgctggtg ccggaacact tttcctcgcg
     3661 gtcgcactgg ccggccgccg cgcgacatac aaaaccggca ccgcgaatca ggtcaacggg
     3721 gcaatccacg ccggttccgg cgcacctttc agcaacgtcg catccaccgg ccgcctcgcg
     3781 gcagacgaat cccttgccct ggatgtagtc cggcggacat gtcgccgaca acccgtcaca
     3841 aatttcttcc cgatcgcatc caccggaggc ctcgcggcaa acagaaccgc ttcgcttcaa
     3901 ctgatccgcg ggacaggaca tcgagcttcc gtcgcaatac tcttcctgat cgcagccgcc
     3961 cgccgccttg cgacaaactg ttgaggctga agccgccttg tccgtcgggc agtcggttcc
     4021 cttgccgtca cacgtttcgg ccgcgtcgca aaggtccgtc gattcacgac agacgtggtt
     4081 ctgatcggaa agcttatccg acggacaatc ggcacccttg ccggtgcaga attcggcggg
     4141 atcgcagtcg ccaccggact tccggcactg cacatcctta cccgaaaaag catttgccgg
     4201 gcattcggcc gaactgccgg tgcaggattc ggcgacgtcg cagacaccat tcgattcacg
     4261 gcaaacggtc gaggccttca ggaaggtatc tgtcggacac tggggcgaag tgccgccgca
     4321 cttttccgcg acatcgcact cgccacggac tgaccgacag gtatatgaag tatccttgaa
     4381 cttgtccgct ggacatgagg tgctgccgcc ggtgcagaac tccgccaggt cgcaatcgcc
     4441 cgccttggcg cgacagacga aggaccccgg cctgtatccg caaccgtcgc aacatgcccc
     4501 gctgctgcac tggcacttcc acccgacgtg gcagaccgtg tccgtgcaga gaccatcctc
     4561 gttatgccgc atcatgcacg gcatgttcag gccacagccc ttcatgcagt tgcggttctt
     4621 gcagccgttg tatccgtcgt tgcacgagca ttccgcatat ccggggctgt cgtattcatc
     4681 gacggcgtaa aaactgtgcc ccatttcatg ggtcgcgacc gtatccatca tcgacaggcc
     4741 atagccgccg ttgccggtcg tcatcatgat cagggggccg ccccagtgcg cataggcgaa
     4801 atacccgtcc gggaacatgt cgtcacggtc gttctggctg ttgacgacaa aaatcgtaat
     4861 cgcgtggtcg gcctcggcgt atcccttcat ataggcattc aaggcatcga ccgaggcggt
     4921 gtaatagcct tccgaatagc caaggttatt caggacatcc gcaatccaga caccttcgtc
     4981 ctgcgaattg tgcgttatcg gttcgtaacc ggtttttact tcatcgtgga cgctgggctt
     5041 gaaaatataa accttgaacg acaggtcaac gtcgtacgat tcggccttct cgggccacca
     5101 ggccatgccc gcgaccactt cgttataaac ctgatcctca agcgtcttcg tccagtcttc
     5161 tcgattcgtg tcgatcgttc cgtcgctttc cggaaggatg atggttacgg ccaccacgtt
     5221 cttcatcggc catgccggca caaccggcac gcccgcggac atgatcccgg gcatatcggc
     5281 aatcccggcg ggcggcactt tcggcgcctg gggaagaaca agcatctgac gcctgtcacc
     5341 ggactgcctc gtgtcgttca ccagtggcgc gaagaactcg ccaccggaag ccttccttga
     5401 agccaggacc gtgccggcca ggaaatccct gtccagttca tcaaccagcc agctttcgcc
     5461 cggaccggaa aaaggcgcca cggatcgaag acggaaatcg cttaaca
//
LOCUS       DAOPPA010000186         5478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16297_length_5478_cov_3.927162, whole genome shotgun sequence.
ACCESSION   DAOPPA010000186 DAOPPA010000000
VERSION     DAOPPA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_5478_cov_3.927162"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKH54_06855"
     CDS             complement(<1..929)
                     /locus_tag="PKH54_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99645.1"
                     /translation="MKKAALVVLVASFATIFMACGAAEDNSCETSENCVFGEVCVDLR
                     CIERPCNGKGDCVTGEVCIPGELVGKEPGMKYCSAPCSELKPCELPLVCGADGICKTN
                     SGTEDIVEDSSEPEDVVRDVAPEVAADVPVELPPIEVEVDCKTCTTTVDCGTGYSCQP
                     VGAEKHCLRDCNNDGDCLAAYTCYASGTQKSCLPVSYNCVPCSFDAPCQDGQCCDFGT
                     GQCKDCRGVCFTCTYDYDCAAGLRCYKETGSANGFCVEECSETECTDTAHFQCNDNGK
                     GVEICQPTTEGCGGCTEPTPFPFEGGCVDCRNN"
     gene            985..1155
                     /locus_tag="PKH54_06860"
     CDS             985..1155
                     /locus_tag="PKH54_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99646.1"
                     /translation="MELPPDIQAGTVKPGKDAELPEIYDLIPRDPALLREMIHSETVT
                     DFTVILFACSRF"
     gene            1239..2585
                     /locus_tag="PKH54_06865"
     CDS             1239..2585
                     /locus_tag="PKH54_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOC99647.1"
                     /translation="MRDPLIIKGLDSPRNIRISAWPSLFMILLLALVNAYADGYDRPA
                     TRPSLDVRLAAIESVPATPEGPFPGLSPERGRGSRSGTRTVNVRLGSGQTPARALTAA
                     GVDAVTAGLALASLSEKIDMRQLKPGQKFIAEVDADGKVVALKYPLSAVDYFEAAVGD
                     EGFQVQKKQLPTDKEIVESACRIDGSLYESFRTCGLDRELVPLVVGLLESQFDLFTDM
                     RRGDTIRLVAIKESLNGNFLRYGRIEGVLYEGKVASAAVFMHEEEDGRVSYWDAAGSS
                     IERPFIRTPIKYNGYTSSQARRRLHPILHAYTPGRAVDYSAPAGTPVMAVADGKVIFA
                     GYKSDHGRMVVIRHGNGIQTYYANLDSIRKGLTKGSVVDRRTQIGTVGTSARAKGSFL
                     HFAVAQNGKFVHPRLLSDFPGVSMGEPQRHDFLAYVGRMTGRLRSLPVRGTEPLTQ"
     gene            complement(2642..3367)
                     /locus_tag="PKH54_06870"
     CDS             complement(2642..3367)
                     /locus_tag="PKH54_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99648.1"
                     /translation="MRTGFIAGTLVLALFAAPFAAGASPVAEKFYKDGVELAKNKQMG
                     EAIAKFQNAVAFEPKTFKYHLNLAYAYDWANRLPEAQAAYEVAMKIGKNAIQAVKGHA
                     DVCRRLKLFSLAENSYRLALKKEPKDLDLIMGLGASMAGQDKLDEALSEYQKAMKLFP
                     KEVQPAFKAANVLRKKGDLDGAIAHYRMAMERDPAYAKAEYGLGLVLRQKEDYPGAKK
                     ALESACKRGVKSSCKFYYKIKDK"
     gene            complement(3364..3861)
                     /locus_tag="PKH54_06875"
     CDS             complement(3364..3861)
                     /locus_tag="PKH54_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOC99649.1"
                     /translation="MMKPLFAPWRMEYVKDAAAPCDCFLCPCRQPNPEGDLVVFRGSK
                     AFVVLNRYPYVAGHVMVVPYRHLSSPLDLESSEMAELGVLIKRSVSAISSVYQPQGFN
                     IGMNVGQAAGAGLKDHIHVHVVPRWNGDTNFVPVIGDVKIIPESLDVTRRRLEIAFLE
                     QEVQT"
     gene            complement(3863..5089)
                     /gene="clpX"
                     /locus_tag="PKH54_06880"
     CDS             complement(3863..5089)
                     /gene="clpX"
                     /locus_tag="PKH54_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOC99650.1"
                     /translation="MCSFCGKPKTSQRRLVSGPGVFICDECVDMCSSLLSTQQERDAS
                     IPAAESGMVRPRDMVKVLDEYVIGQEYAKKVLAVAVYNHYMRIRNGSRRIGRAVEIQK
                     SNILMLGPTGSGKTLLAQTLAKVLNVPFTIVDATCLTEAGYVGEDVENIILALLQAAD
                     WNIEQAQQGIIYVDEIDKIARKADSPSITRDVSGEGVQQALLKILEGTVANVPPKGGR
                     KHPQQEYIRVDTTNILFICGGAFVGLDEIVSERLRGGTIGFGTRVEARKKDSIGDILR
                     DTEPEDLIKFGLIPEFVGRFAVTASLDLLDEHALVRILSEPRNALIKQYQAIFEMEGI
                     QLEFSEDALGAVARQAVARGTGARGLRSIMERVMLDLMFDLPGNKEVSRVLIGEDVVS
                     GKGGPVIEYGGKAKTA"
BASE COUNT         1140 a         1645 c         1530 g         1163 t
ORIGIN      
        1 tcgttgttgc ggcaatcgac gcagccaccc tcgaacggga acggggtcgg ctcggtgcag
       61 ccgccgcagc cttcggtcgt gggctggcaa atctcgacgc ccttgccgtt gtcgttgcac
      121 tggaagtggg ccgtgtcggt acattccgtc tcgctgcatt cctcgacgca gaaaccgttg
      181 gcgcttcccg tttccttgta gcagcgcagg ccggcggcgc agtcgtaatc gtatgtgcag
      241 gtgaagcaca cgccacggca gtccttgcac tgaccggtgc cgaagtcgca gcactgtcca
      301 tcctgacagg gggcatcaaa gctgcacggc acgcagttgt acgaaaccgg caggcagctc
      361 ttctgggtgc cggaggcgta gcatgtgtag gcagccaggc agtcgccgtc gttgttgcag
      421 tcgcgcaggc agtgcttttc ggcaccgacc ggctgacagg agtacccggt tccgcagtca
      481 accgtcgtcg tgcaggtctt gcagtcaact tcgacttcaa tcggcggaag ttcgaccggg
      541 acatccgcgg ccacttcggg agcgacatcg cgcacgacgt cctcgggctc gctggagtct
      601 tcgacaatgt cttcggtgcc gctgttggtc ttgcagatcc cgtcggcgcc acagaccaga
      661 ggcagttcgc acggcttcag ttcgctgcac ggagccgagc agtacttcat gccgggttcc
      721 ttgccgacca gttcaccggg gatgcagact tcaccggtca cgcagtcgcc cttgccgttg
      781 cacggacgtt cgatgcagcg cagatcgacg caaacttcgc cgaacacgca gttctcactg
      841 gtctcacagg aattgtcctc ggcagcgccg cacgccatga aaatcgtggc gaaagaggcg
      901 accaggacca caagagcggc cttcttcatt ttccttctcc tgacagaatt tggaacgaaa
      961 caatagcaaa gccaaggtat tataatggaa ctgccaccgg acattcaagc aggaactgtc
     1021 aaaccgggga aagatgcgga acttcccgaa atttatgact taattcccag agatcccgct
     1081 ttgcttcgag aaatgattca ttctgaaacg gttaccgatt tcactgtgat tctgtttgct
     1141 tgcagccgct tttgaacatc cccgatgatg aacggcgatt gacgcggcgg gcgtcgggcc
     1201 ggataatctg gccgacctgg catccgtgaa ggggccgcat gcgtgatcct cttatcataa
     1261 aaggactgga cagtcccagg aacatcagga tttcggcctg gccgtcgctc ttcatgattc
     1321 ttctgctggc cctggtcaac gcctacgctg acgggtatga ccggcctgcg acacgcccct
     1381 cgcttgatgt ccgccttgcg gccatcgaat cggtccccgc gaccccggag ggcccgttcc
     1441 cgggactcag cccggagcgc ggacgtggct cccggtcggg aaccagaacc gtcaacgtca
     1501 ggctcggaag cggccagacc ccggccaggg ccctgaccgc ggccggcgtg gatgcggtga
     1561 ccgccggact tgccctggct tccctttccg aaaagattga catgcgtcag ctcaaacctg
     1621 gccagaagtt catcgccgaa gtcgatgccg acgggaaggt ggtggcgttg aagtatcccc
     1681 tcagcgcggt tgattacttc gaggccgccg tcggcgacga agggttccag gttcagaaga
     1741 aacagcttcc gacggacaag gaaatcgtcg agtcggcttg caggatcgac ggttccctgt
     1801 acgaatcctt ccgaacctgc ggactggatc gcgagctggt tccacttgtc gtcggccttc
     1861 ttgaaagcca gttcgacctt ttcaccgaca tgcgccgcgg cgacacgatc aggcttgtcg
     1921 cgatcaagga aagcctcaac ggcaacttcc tgcggtatgg ccggatagaa ggagtcctgt
     1981 atgaaggcaa ggtcgcctcg gccgcagtat tcatgcacga ggaggaggac ggacgggttt
     2041 cgtactggga tgccgccggt tcgtcgatag agcgtccttt cataaggacg cccatcaagt
     2101 acaacggcta cacgtccagc caggcaaggc gcaggctgca cccgatactg cacgcatata
     2161 ctccggggcg ggccgtcgat tactccgctc cggccggaac ccccgttatg gcggtggccg
     2221 acgggaaggt gatcttcgcg ggatacaaga gcgaccacgg caggatggtc gtcatcaggc
     2281 acggcaacgg gattcagact tactacgcca atctggactc aatcaggaag ggcctgacaa
     2341 agggttccgt cgtcgatcgc aggacgcaga tcggaaccgt tggaacttcg gcgagggcga
     2401 agggctcgtt ccttcacttc gcggtggccc agaacggaaa atttgttcac ccgcgacttc
     2461 tttccgattt tccgggagtg tcgatggggg aaccgcaaag gcatgatttc ctggcgtacg
     2521 tcggaaggat gacagggcgt ctcaggtcgc ttccggtccg gggaactgaa ccgctgactc
     2581 agtagggcgc gagcccttgt aacgcgggga cttcccagac aggacgggcg cgtgattcga
     2641 atcacttatc cttgatcttg taatagaact tgcacgagga cttcacaccg cgcttgcagg
     2701 cggattcaag ggccttcttc gcgcccgggt agtcttcctt ctggcgcaga acaagcccca
     2761 gtccatattc ggccttcgcg tatgccgggt cgcgctccat ggccatccga taatgcgcga
     2821 tcgctccatc aaggtcgccc ttcttcctta acacgttggc cgccttgaat gccggctgaa
     2881 cctccttcgg gaacaacttc atcgccttct ggtactcgct cagcgcctca tcgagcttgt
     2941 cctggccggc catggatgcc ccgaggccca tgatcaaatc aagatccttc ggctccttct
     3001 tcagggcgag ccggtatgaa ttctcggcaa ggctgaacag tttcaggcga cggcagacat
     3061 cggcgtgtcc cttgaccgcc tgaatcgcgt tcttgccaat cttcatcgcg acttcatacg
     3121 cagcctgggc ctcgggcaga cggttggccc agtcataggc atacgccagg ttcaggtggt
     3181 acttgaaagt cttcggctcg aacgcgacag cattctggaa tttggcgatt gcctcgccca
     3241 tctgcttgtt cttcgccaac tcgactccat ccttgtagaa cttctcggcg accggcgacg
     3301 ccccggccgc gaacggggcc gcgaacagcg ccagcacaag cgtacccgcg atgaaacctg
     3361 tcctcatgtc tgaacctcct gctccaagaa agcgatctcc aggcgtcgcc tggtaacgtc
     3421 gagcgactct ggaattatct tcacatcgcc gataaccgga acaaaattcg tgtccccgtt
     3481 ccatctggga accacgtgaa cgtgtatgtg atccttcagg cccgcgcccg ccgcctgtcc
     3541 cacgttcatc ccgatattga atccctgggg ctgataaaca gatgagatgg ctgaaaccga
     3601 cctcttgatc agcaccccga gttccgccat ttcggaagat tccagatcaa ggggactcga
     3661 aagatgcctg tagggaacaa ccatgacgtg cccggcgaca taaggatacc tgttaagcac
     3721 cacgaatgcc ttcgaaccgc ggaagaccac aagatcacct tcaggattcg gctgacggca
     3781 cggacacagg aagcagtcgc acggtgccgc cgcatccttg acgtattcca ttctccaggg
     3841 tgcgaaaagg ggcttcatca tttcacgccg tctttgcctt gccgccgtat tcgatgacgg
     3901 gaccgccctt gccggaaaca acgtcctccc cgatcagaac ccgcgagact tccttgtttc
     3961 cgggaagatc gaacatcagg tcaagcatca cgcgttccat aatggatctt agtcctcgag
     4021 ccccggtgcc cctggccact gcctgccggg cgaccgcccc cagggcatct tcggagaact
     4081 cgagctgtat cccttccatc tcgaaaatcg cctggtactg cttgatcaag gcatttcgcg
     4141 gttcggaaag aatcctgaca agggcatgtt cgtccagcaa atcaaggctg gccgtaaccg
     4201 cgaaacgacc gacgaattcg ggaatcaggc cgaacttgat caggtcttcc ggttcggtgt
     4261 cgcgaaggat gtcgccgatg gaatccttct ttcttgcctc gaccctggtg ccgaaaccaa
     4321 tggttccgcc gcgcagcctc tcggacacaa tttcatcaag accgacgaat gcgccgccgc
     4381 agatgaacag gatattggtg gtatccaccc ggatgtattc ctgctgcgga tgctttcttc
     4441 cacccttcgg tggaacgttg gcaacggttc cctcaaggat tttcagcagc gcctgctgca
     4501 cgccctcccc cgagacgtcg cgggtgatcg acgggctgtc cgccttgcgg gcaatcttgt
     4561 cgatttcgtc aacgtagatg atgccctgct gtgcctgctc aatgttccag tcggcggcct
     4621 gaagcagggc caggatgatg ttttcgacat cctccccgac atatcccgcc tcggtcaggc
     4681 atgtggcgtc cacgatggtg aacggcacgt tcaatacctt cgcgagggtc tgcgcaagaa
     4741 gggttttgcc gcttccggtc ggcccgagca tcagaatatt gcttttctgg atctcgactg
     4801 cccgcccgat acgccggctg ccgttcctta tcctcatgta atgattgtaa actgcgacgg
     4861 ccagaacctt cttcgcgtat tcctgaccta taacgtattc gtcgagaacc ttgaccatgt
     4921 cccgtgggcg aaccattccc gattcggccg ccggaatgga ggcatcacgt tcctgctggg
     4981 tggaaagcag cgaggaacac atatcgacac attcgtcgca gatgaataca cctggccctg
     5041 aaaccaggcg tctctgggaa gtcttcggtt ttccacagaa agaacacatc ggccttccgg
     5101 attttgttcc gttgtccaat accatctgtc acacactccg tcgggatggc agaaccacat
     5161 tccgaccttg aaaaaaaaac cggtcgtggg ggccggtcgc ccatattgta ctttcaaggg
     5221 atggtgttgg caaactgacg gggttgtcgg aaaaccggtt caaaaccctg tagcaaaatc
     5281 aaaggtgaaa gcgggaacga cggttcaaaa catgtggatg tttcattcct gccgctgcag
     5341 gctgttcaaa caaggtcgtc aatcgaagat gatgacacag aaccatgaac agcggcttat
     5401 cgtgtcaaaa tccaatcatt acgaatcatt tcgattgaag atgaacatcg ttgaacaacc
     5461 ttgataccct tatctgct
//
LOCUS       DAOPPA010000187         5470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16327_length_5470_cov_4.307110, whole genome shotgun sequence.
ACCESSION   DAOPPA010000187 DAOPPA010000000
VERSION     DAOPPA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16327_length_5470_cov_4.307110"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PKH54_06885"
     CDS             complement(<1..953)
                     /locus_tag="PKH54_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99651.1"
                     /translation="MTSESRPEQAKNPRSWVFSTYFAEGLPYMLVRYLSSVYLTDIGV
                     REAYLGFVNFLGIPWNFKFLWAPAVDLFATRRKWLLVVELALVAGFAIVGLLAAAGPD
                     VTAGTAAPAADGMQSLAVRSTIVLLVMLAFLSATHDIAIDGFYMEAIDDPTRQAAYTG
                     LRVLAYRLAVIFTKSVLVAMAGWVSWTAGWIGGAAALGLLLLYHWRMLPNPTGADRTA
                     RDGAAIARDWGRAFTTYLSQPRAWLVLLFVVTYKLGDEVLFSMNTPFLMRELGVTKPQ
                     LSWVSGVFGTVASIAGSLVSARAISKWGLGRAVWPLTLAMNL"
     gene            1067..1894
                     /locus_tag="PKH54_06890"
     CDS             1067..1894
                     /locus_tag="PKH54_06890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC99652.1"
                     /translation="MKIQEAITFLAGQFTAIDDFRGQGLNRHRSPEFKTNVAALVACV
                     VLFGNNFFVIGGPAQKWVMQNAIDFVVSMQPGDLRATLLDVVSISRNAYWSLGCLFFY
                     FVIPALVIRLVFREKLSEYGLSPKGFFKHLPIYLLLFLPVAAGTVLVSYSDSFQRTYP
                     FYHNPRFIWDMLLWDFLYCVQFFGLEFFFRGFMVHSMKFKLGSSAVWVMVLPYLMIHF
                     TKPAPEAFGAIIAGTVLGVLSLRTCSIWGGVFIHCMVAIMMDWLSLIHRGAFPSILE"
     gene            complement(2100..3116)
                     /locus_tag="PKH54_06895"
     CDS             complement(2100..3116)
                     /locus_tag="PKH54_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99653.1"
                     /translation="MKSLIAFIFARARVIVFFIWVYALIELLQGGRYLTFLRPEFGYV
                     LGAALAMFLAVMIAEMGREPGDRLHWSAFQRPLILLIPLLFLLNAQGASLDYYTFNKR
                     FTGTAGMAVNVEPSDDSGVPAGGRAAVDNGQAGEAGTVSADDADVPSSLNPSKTDRIR
                     FSRKWNQDGTPGNASGTPSTGSGTDVEAGSQPPQQPPHEPGTLPEDQRPKGDNDDEAD
                     DDGTPDVIETALTSLYETPKLFEGKQVSVIGMVDTNEDIARQFGDRAKVLFRFLISCC
                     AADAQPIALIFETKQSTTIKGENVWIRVSGKFTLHKKDGRTIPVIREASLSTVPRPAN
                     EYLY"
     gene            complement(3113..4240)
                     /locus_tag="PKH54_06900"
     CDS             complement(3113..4240)
                     /locus_tag="PKH54_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC99654.1"
                     /translation="MKAKVRPIGMRSFIFETLFLVATVTLFFAGQAMESMRTLSLIFA
                     SIVLEAVPFMLLGAVVGGLLETFVSRERIASLLPRKRWKTVLIAAGLGVIFPVCECAI
                     VPIVRRLSRKGLPMAGAVAYLLGGPIANPIVAGSTLVAYKLEWPMMAARFIGGYLIAV
                     AIGLAMDRIFRKRQAFVDDVAMPGAGAAGCACGHDHDHQDEHDGESGHEHDHEHEGGQ
                     TCACGHHHAAPKGPRFIDKMRAAMLHAADDFLGVGHYLVIGAFIAAIAQTYIDRTVFT
                     QIFQIPVLSILMMMALAILLNLCSEADAFIAASFRGIMPMSAQLAFLLTGPMFDLKLL
                     LMYQGLFRKRAIAALASLILVSAALVSIAAHYSGFFTKVMP"
     gene            complement(4237..5103)
                     /locus_tag="PKH54_06905"
     CDS             complement(4237..5103)
                     /locus_tag="PKH54_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOC99655.1"
                     /translation="MPDLSPILAVIGALIPLECMQAGFMQRAMLGLLLLAPMTAAMGV
                     QVINLRMAFFSDAISHSAFAGIALGALFSVHPHWSMPLLGVAVGIGIVAIGRHSMLSS
                     DTVIGVFFAAVTAGGIALVSRDPSLARDTQIFLYGDILTIADVDLLQLGGLLLLFSAF
                     QILAFNRILYICINPDLAEVHRVKVWAYQYLFAALLALVVIFSVWAMGVLLVTAMLIV
                     PAATARNLARSSGTMFWWAVGISVSSAVGGLLLSAHQSIRTATGATIILISFTWFLVS
                     FVPVALRKEKAR"
     gene            complement(5096..>5470)
                     /locus_tag="PKH54_06910"
     CDS             complement(5096..>5470)
                     /locus_tag="PKH54_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC99656.1"
                     /translation="ALSGGELQRVLLALAFQQDPDLLILDEPAAGVDFRGGQIFCELL
                     ENLRAEHGFTQVMVTHDLGLAASHATYAILLNGRVIAEGNPTVTLNPENLTRAFGTHM
                     GGAVIGPLSHSCGCEGTGAGNA"
BASE COUNT         1149 a         1607 c         1632 g         1082 t
ORIGIN      
        1 aggttcatcg caagcgtcag cggccagacg gcgcgcccaa gtccccattt tgatatcgcg
       61 cgggcggata ccagcgaccc tgcgatcgaa gccaccgtgc cgaagacccc ggaaacccat
      121 gacaactgtg gctttgtcac ccccagttcg cgcatcagga acggggtgtt catggaaaac
      181 aggacctcgt cgcccagctt gtaggtcacc acgaacagaa ggacaagcca ggctcgtggc
      241 tgggaaaggt aggtcgtgaa ggcccgtccc cagtcccggg caatggcagc gccatcgcgg
      301 gccgtcctgt cggccccggt cgggttcggc agcatccgcc agtggtacag aagcagcagt
      361 ccaagcgctg cggcaccccc gatccagccg gctgtccatg agacccatcc ggccatggcg
      421 accagcacgc tcttggtgaa gatcacggca agccgatacg ccagcacccg caggcccgta
      481 tatgccgcct gcctggtcgg gtcgtcgatt gcctccatgt agaagccgtc aatcgcgata
      541 tcgtgggttg ccgaaaggaa tgccagcatc accaggagca cgatggttga cctgacggca
      601 agcgactgca ttccatcggc cgcgggtgct gccgttcctg ccgtgacatc cggaccggcc
      661 gcggccagca gcccgacaat ggcgaatccg gcgaccaggg caagttccac gaccagcaac
      721 cattttcgtc gcgtcgcgaa cagatcgacc gccggtgccc acaggaactt gaaattccag
      781 gggatgccca ggaaattgac gaatccaaga taggcttccc ggacgccgat atctgtcaga
      841 taaaccgaag aaaggtaccg caccagcatg tacggcagcc cctcggcgaa atacgtggag
      901 aaaacccagg accttgggtt tttcgcttgc tcgggcctgg attcactggt catccgcgat
      961 ccggtctgaa gctgggtcaa ggttatgccc gcaccggctt cggtgcaaag tccgcgcctt
     1021 caaaggttgt tgcgacttgc cttgcgcaag gctagaatcc cgtcccatga aaatccagga
     1081 agcaatcacg ttccttgccg gccagttcac cgcgattgac gatttccgcg gtcagggttt
     1141 gaaccgccat agatccccgg agttcaagac aaacgtcgcg gcgctggtcg cgtgcgtcgt
     1201 tctgtttggg aacaatttct tcgtgattgg cgggcctgcc cagaagtggg tgatgcagaa
     1261 tgcgatcgat ttcgtcgttt ccatgcagcc cggggatctt cgcgccacgc tgctggacgt
     1321 cgtttcaatc agcaggaacg cctattggtc gcttggttgc ctgttctttt acttcgtaat
     1381 tcccgccctg gtcatccgcc tggtcttccg ggaaaaactc tccgaatacg gcctgtcgcc
     1441 gaaaggcttt ttcaagcact tgccgattta cctgctgctg ttcctgccgg tcgccgcggg
     1501 caccgttctt gtcagttatt ccgattcctt tcaaaggact tatccgtttt accacaaccc
     1561 ccgtttcatc tgggacatgc tgctgtggga tttcctttat tgtgtccagt tcttcggcct
     1621 tgagttcttc ttccgtggat tcatggttca ttccatgaag ttcaagctgg gttcctcggc
     1681 cgtctgggtc atggttcttc cgtacttgat gattcatttc accaagcccg cgccagaggc
     1741 cttcggtgcc atcatcgcgg gaactgtgct gggcgtcctc tcactgcgca cgtgcagcat
     1801 ctggggtggg gtattcatcc actgcatggt cgcaatcatg atggactggc tttcgctgat
     1861 tcatcgcggg gcattcccgt ccattcttga gtaggacgcg gcatttcggc ttcaggccgg
     1921 cggcgttgcc gagcgacacc atgctgcgga cgggaacctg gaaccccgcg aatcgtgcgc
     1981 atcaatcatg aaggtcatcg attcagtaca ggaaagggat cgtcagaagg catcggtaaa
     2041 tgcatcggca aaatgcatcg gtaaaggcat cgtcaaaagg catcggtaaa ggcatcggtc
     2101 tagtagaggt attcgttcgc cggtctgggg acggtgctca aggaggcttc acggatcacg
     2161 gggatggtgc ggccgtcctt tttatgaagg gtgaattttc cgcttacgcg aatccagacg
     2221 ttttcaccct tgatggtcgt ggactgtttt gtttcaaaaa tcagggcgat cggctgggca
     2281 tcggccgcgc agcatgaaat caggaacctg aagaggactt ttgcccggtc gccgaactgg
     2341 cgcgcgatgt cttcgttcgt gtcaaccatg ccaatcactg atacctgctt gccctcgaaa
     2401 agcttgggcg tctcgtaaag cgatgtcagg gccgtttcga ttacgtccgg cgtgccgtcg
     2461 tcgtcagctt cgtcgtcgtt gtcgcccttc gggcgttgat cttcaggaag cgtgccaggt
     2521 tcgtgcggtg gctgctgtgg cggctgggag ccggcctcga cgtccgtccc ggatccggtt
     2581 gacggggtgc ctgaagcgtt gcccggtgtc ccgtcctggt tccacttgcg gctgaacctt
     2641 atccggtcgg tctttgacgg gttcagggaa gagggaacgt ccgcgtcgtc agccgaaacg
     2701 gtgccggctt ccccggcctg gccgttgtca acggccgccc ggccgcccgc ggggacgccc
     2761 gaatcgtcag aaggttccac attgaccgcc atcccggcag ttcccgtgaa tcgtttgttg
     2821 aaagtgtagt agtcgagtga cgcgccctgg gcgttcagca ggaaaagaag cggaatcagc
     2881 agtatcagtg ggcgctggaa ggccgaccag tgaagtcggt cgcccggttc gcgccccatc
     2941 tcggcgatca ttaccgccag gaacatggca agcgccgcgc caagcacata gccgaattcc
     3001 ggtcgcagga aggtcaggta acgcccaccc tgaagaagct cgatcagggc atatacccag
     3061 atgaagaaaa caatgactct ggcacgcgcg aagatgaatg caatcagcga cttcatggca
     3121 tcaccttcgt gaagaagccg ctgtaatggg ccgcaatcga gacaagcgcg gccgaaacaa
     3181 ggatcaggga tgccagggcc gcgatcgccc tcttgcggaa aagtccctgg tacatcagca
     3241 gcagtttgag gtcgaacatc ggacctgtaa gaaggaaggc gagttgcgcc gacatgggca
     3301 tgattccacg gaaggatgcc gcgatgaatg cgtcggcctc cgagcacagg ttcaaaagga
     3361 tcgcgagcgc catcatcatc aggatcgaaa gaaccggaat ctggaagatc tgcgtgaata
     3421 ccgtgcgatc gatgtacgtc tgggcaatgg ccgcgatgaa cgcgccgatc acaaggtaat
     3481 ggccaacccc aaggaaatcg tcggccgcgt gcagcatggc ggcacgcatt ttgtcaataa
     3541 aacgcgggcc cttcggcgct gcgtgatggt ggccgcaggc gcatgtctga ccgccttcat
     3601 gctcatggtc gtgttcgtgg ccgctttcgc catcgtgttc gtcctggtgg tcgtggtcgt
     3661 ggccgcacgc gcatccggcc gcgccggcgc caggcatcgc gacgtcatcg acgaaagcct
     3721 gccttttcct gaaaatgcga tccatggcaa gaccgatggc aaccgcaatc aggtagccgc
     3781 cgatgaatct tgccgccatc atcggccatt ccagtttgta tgcgaccagc gtcgaaccgg
     3841 ccacgattgg gttcgcgatc ggtcccccaa gcaggtacgc gacggccccc gccatcggca
     3901 ggccctttct tgagagcctt ctgacaatcg gaacgattgc gcattcgcac accgggaaga
     3961 tgacccccag gccggccgca atcagaaccg ttttccagcg tttacgggga agcagcgacg
     4021 cgattctttc gcggctgacg aaggtttcaa gcagcccgcc gacgacggcg cccagaagca
     4081 tgaatggcac ggcctccagg acgatgcttg cgaagatcag cgaaagcgtg cgcatcgatt
     4141 ccatcgcctg tcccgcgaag aacagggtga cggtggcgac aaggaacagt gtttcgaaaa
     4201 tgaacgaccg cattccgatc ggtcggactt ttgctttcat cgggcctttt cctttcgaag
     4261 cgcgacaggc acgaagctta ccagaaacca tgtaaatgaa atcaggatga ttgtcgcgcc
     4321 ggttgccgtc ctgatcgact gatgcgcgga aagcagcagt ccgccgacag ccgaagaaac
     4381 gctgattccg acggcccacc agaacatggt tccgcttgac cttgcaaggt tgcgggccgt
     4441 ggccgccgga acgatgagca tcgcggtcac cagcaggaca cccatggccc acacggaaaa
     4501 gatgacgaca agcgccagga gcgccgcgaa caagtactgg tatgcccaga ctttcacgcg
     4561 gtggacttcg gccaggtcgg ggttgatgca gatgtaaaga atccggttga acgcaagaat
     4621 ctggaaagcc gaaaacagga gcagaagtcc gccaagttga agcagatcaa cgtccgcgat
     4681 ggtcaggatg tcgccgtaaa ggaaaatctg ggtgtccctg gcaagggaag gatccctgct
     4741 gacaagcgcg attcccccgg cggtgaccgc cgcgaagaac acaccgatga cggtgtccga
     4801 cgacagcatc gaatgtcgtc cgatggccac tattccgatg ccgacggcaa ccccgagcag
     4861 cggcatagac cagtgcggat gcaccgaaaa cagcgcgccc agggcgatcc cggcgaatgc
     4921 ggaatggctt atcgcgtccg aaaagaaggc catccgaagg ttgatgacct ggacgcccat
     4981 ggcagcagtc atgggcgcga gcagcagaag tcccagcatg gctcgttgca tgaagccggc
     5041 ctgcatgcac tcaagcggta tcagggcgcc gatgacggca agaatcgggc tcaagtcagg
     5101 cattgccagc ccccgttcct tcacatccgc acgaatgcga cagcggaccg atgacggccc
     5161 cgcccatatg cgttccgaaa gcccgggtaa gattttccgg gttaagcgtt acagttggat
     5221 taccttccgc gataacgcgt ccgttcaaca ggatggcgta tgttgcgtgg cttgcggcaa
     5281 ggcccaggtc gtgcgttacc atgacctgcg tgaagccgtg ctcggcccgc aggttttcaa
     5341 gcagttcgca gaagatctgg cctcccctga aatccacgcc ggcggccggt tcgtcaagaa
     5401 tcagcagatc cggatcctgc tgaaacgcca gtgcaagcag gactctttgc agttcgccgc
     5461 cggagagagc
//
LOCUS       DAOPPA010000188         5444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16435_length_5444_cov_4.269994, whole genome shotgun sequence.
ACCESSION   DAOPPA010000188 DAOPPA010000000
VERSION     DAOPPA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_5444_cov_4.269994"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PKH54_06915"
     CDS             <1..1118
                     /locus_tag="PKH54_06915"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HOC99657.1"
                     /translation="FTECYREMGESRCFGTRTCDTECDAPFPATESCNGIDDDCDGQT
                     DEEGAQGCTNFFLDSDGDGYGTGQYKCLCAASGSFRAVRACQGTTTCDCRDDVFGVNP
                     GATEICGNGYDDDCNGIQDTENAQGCNPYLFDGDNDGFGRNDISKCLCAPDGNYRTLV
                     GNDCRDDLQAVNPGAYEKCNNGLDDDCDGRIDNKPGDTNAICNVCGDGWIDNMEACDH
                     GNQNVGAACSSDCRSVACGSEQYANITTLLGTTCYWRSTDAIARSNAETRCGERGGFL
                     VRLESASERDWVFDNLIFPPGSGSRVWIGARNSNGAWRWDDGTPVGDEFVWRPGEPSG
                     NGDCVQSSGSGINYDDLPCTGQNRDYVCERPKVGTPR"
     gene            complement(1121..3229)
                     /locus_tag="PKH54_06920"
     CDS             complement(1121..3229)
                     /locus_tag="PKH54_06920"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOC99658.1"
                     /translation="MNTLSWGRMLSCLGLVAVMAAVPGCGGGSEPEDDLGGTDVASDS
                     IGDDVEVPDHGPRPDLNVPDNQNDPGGQDAGETEEPVDLGIDPGELDILPDLHLTDVI
                     DPVCNDPDGDGFGNGCEAGPDCAPSDASRYEFVQYYIDSDFDGFGAGELHSVCSADGV
                     PPGYSLVNTDCDDGNAAVFPGAPDNPDDNKTSDCGGEDIKAATADGIFVKPGNADTNP
                     GTRESPVGTLSAAVSLAVSGGKSHIFVATGTISDSVTIVSDIQIHGGYDATTWTRAAQ
                     KSRLLGTADSAVVISGSTVAIDQFEIFGTQDGTIVNAADKVVGIMINDSNVFLTNVVA
                     GGSSVQTSGVDGRDIVSAGVYIQSSDVTMNECRIDDGGPMIGKYSGTGSVDRRITSRA
                     IMLVSGNLRMNGGTAGFYPEIELSDIKGAAHAQATSRGILVAGGRAVVTGVDFSGGID
                     VTIDDGGDDGTTGQATAIGDGAGIEVTGGEAWVINSVIGGGMVKTYVELDTVGTVENP
                     IDAVATARTVAVPVLATGGKLVVAHCDLFTERFSEAVVEATSSRGQEVVSATNLTGLV
                     KVDAGANAVVVNSAGYSQINVDDSALLSIDPQATVAMINSVWWDMEYGVCRVRAGEAC
                     VIAEGGIEGLAEIDGVTEAEGNLIADQLWGWWDEVDPGSPLKDGGVDPLTRGLGVFQD
                     LAGTARPQGGAWDIGAYEFN"
     gene            complement(3333..4682)
                     /locus_tag="PKH54_06925"
     CDS             complement(3333..4682)
                     /locus_tag="PKH54_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOC99659.1"
                     /translation="MNHHSSLMRIIPLMMLAVVAGGCGANGGTDTVDSPDAAVELIDN
                     DVSDPGDADPRDQANVDSTADASDASDLPTDVANEVAVDALADSAIDGNVDPDADLPD
                     ADPDAAGDTGPDAGDPDGFIEDALADAWSEILEEVFDDIFEDVPERDQHVADDGPDVS
                     DDVPVTVDNGGSDISTPGLPVRIVAANVTSGNYQSYDYGHGIRIMQALRPDIVLIQEM
                     NYGDNTASDYNELAQDVVGTSYWAVDDAGFQIPNGVLSRWPITGSGWWDDPNLSNREL
                     MWATIDIPGPVDIFAISVHLHTSPSSDQVEAAQVIVGKVKTHRAQNQGKYLYVVGGDF
                     NGTSAVSESGFGQDGVFMVDPPDPADDDGNVNTNASRSKQYDFVLADQKMAGYQVGVR
                     YQSNSTGPDRVYPEGLVFDTRTFSQSELNEYFPPAQTGDSSSSQMQHMAVVKDFVIP"
     gene            complement(4850..>5444)
                     /locus_tag="PKH54_06930"
     CDS             complement(4850..>5444)
                     /locus_tag="PKH54_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99660.1"
                     /translation="LAMVPVNPPPEVTEDPWEDPDAHQPVPGTVCGLFLDDSVAGGYR
                     LETFQDAAAAQEAGAFITHWDGCGVCSTLKNLVVYMRQPDLTDPVRQCGLDNFGNKDG
                     NIQCLMDLGFELPCAQIWYYNTLHTQQECFAPCIAALDQPYHNPDGSLNECLVCDEVK
                     SGPVFKAVAGRNRRNTGLPSSMCRPCQEVQPVFHFYR"
BASE COUNT         1161 a         1677 c         1583 g         1023 t
ORIGIN      
        1 tcttcaccga atgttaccga gagatgggcg aatccagatg ctttggaacc agaacgtgcg
       61 acaccgagtg cgatgcgccc ttccccgcca ccgaatcgtg caacggcatc gatgacgact
      121 gcgacgggca gaccgacgag gaaggagccc agggttgcac caacttcttc ctggactctg
      181 acggcgacgg atacggaacc ggacaataca aatgcctgtg cgcggccagc ggctccttca
      241 gggccgtccg cgcgtgccag ggcacgacaa catgcgactg ccgcgacgat gtattcgggg
      301 tcaacccggg ggcaaccgag atctgcggca acggttacga cgacgactgc aacggcatcc
      361 aggacacgga aaacgcgcag gggtgcaatc cgtatctttt cgacggcgat aatgacggct
      421 tcgggcgcaa cgacatatca aagtgcctgt gcgcgccgga cggaaattac cggacgcttg
      481 tcggcaacga ctgtcgggac gacctgcagg ccgtcaaccc cggggcttat gagaagtgca
      541 acaacgggct ggacgacgac tgtgacggcc gcatagacaa caagccgggg gatacgaacg
      601 cgatctgcaa cgtctgcggc gacggatgga ttgataacat ggaggcctgc gaccacggga
      661 accagaacgt cggcgccgcc tgctcatctg actgccgatc ggttgcgtgc ggatccgagc
      721 aatacgcgaa catcacgacg ttgcttggca ccacctgcta ttggcgaagc accgacgcaa
      781 tcgcaaggtc aaatgccgaa accaggtgcg gcgaacgggg cggcttcctg gtcaggctgg
      841 aatcggcttc ggaacgcgac tgggtatttg acaacctgat cttcccgcca ggcagcggca
      901 gtcgagtctg gattggggcc aggaattcca atggcgcgtg gcgatgggat gacggcacgc
      961 cggtcggcga cgagttcgtg tggagaccgg gagaaccaag cggtaacggt gactgcgtcc
     1021 aatcgtccgg aagcggcatc aactacgatg acctgccttg caccggacag aaccgcgatt
     1081 acgtatgcga gcgccccaag gtcggcaccc caagataact tcagttgaac tcataggcgc
     1141 cgatgtccca cgccccaccc tgcggacgcg cggttccggc aagatcctga aacactccca
     1201 ggccacgagt cagcgggtca acgccgccat ccttcagcgg gcttccggga tcgacttcgt
     1261 cccaccatcc ccagagttgg tcggcaataa gattcccttc ggcctcggtt accccgtcga
     1321 tttcggcaag gccctcaatt ccgccctcgg cgatcacgca ggcctcgccg gcccgaacac
     1381 gacatacgcc gtattccatg tcccaccata ccgaattgat catcgccacc gtcgcctggg
     1441 gatcaatgga cagcaaggcg ctgtcatcaa cgttaatctg gctgtaacct gccgaattga
     1501 caacgaccgc gttcgcgccc gcatcgactt tgaccagccc tgtcagattt gtcgccgaaa
     1561 ctacttcctg gccgcgggat gaagttgcct cgaccacggc ctcgctgaag cgctcggtga
     1621 acagatcgca gtgcgcgaca accagttttc caccggttgc cagcaccgga accgcgaccg
     1681 tgcgtgccgt ggcgactgca tcgattggat tttccactgt gccgaccgta tccagttcga
     1741 cataggtttt caccatgccg ccgccgatga ccgaattaat gacccatgcc tcgccaccgg
     1801 tcacctcgat tcccgcgcca tcaccgatgg ccgtggcctg gcctgtggta ccgtcgtcgc
     1861 cgccgtcgtc aatagtcacg tcaatgccac cggaaaaatc gacaccggtg acaaccgccc
     1921 tgcccccggc aacgagtatg ccgcgcgatg tggcctgggc gtgggcagca cccttaatgt
     1981 ccgaaagttc gatttccgga tagaagcctg cggttccacc gttcatgcgc aggtttccag
     2041 acaccagcat gatggcacgg ctggtgatcc tgcgatccac gctgccggtg ccgctgtact
     2101 tgccgatcat cgggccaccg tcgtcaatcc tgcactcatt catggtcacg tcggaggact
     2161 gaatataaac gccggcggaa acgatatccc gaccatcgac gccggatgtc tggacagagg
     2221 agccaccagc gacgacgtta gtcaggaata cgttggaatc attgatcatg atcccgacca
     2281 ctttatcggc cgcattcaca atcgttccgt cctgggttcc gaaaatctcg aactgatcaa
     2341 tcgcgactgt cgaaccgctt atcaccacgg cactatcggc ggttccaagc agccttgact
     2401 tctgggccgc cctggtccac gtcgtcgcgt cgtagccgcc atgaatctga atgtcagaga
     2461 caatggttac cgaatcgctg atagtgcccg tggcgacaaa aatgtgcgac ttaccgcccg
     2521 aaacggcaag agaaacggct gccgaaagcg tgccgaccgg ggattcgcgg gttcccgggt
     2581 tggtgtcggc gttacccggc ttgacgaaaa ttccgtcggc ggtcgcggcc ttgatgtctt
     2641 cgccgccaca gtcgcttgtc ttgttgtcat cgggattatc aggggcacca ggaaacacgg
     2701 ccgcattccc gtcgtcgcaa tcggtgttga ccaacgaata accgggcggc acgccgtcgg
     2761 cggaacaaac ggaatgcagt tcgcccgcgc cgaagccatc gaaatcgctg tcgatgtaat
     2821 actgaacgaa ctcataccgc gaagcatccg aaggcgcgca gtccgggcct gcctcacatc
     2881 cgttgccgaa gccgtcgcca tccgggtcgt tacaaacggg atcgatgaca tccgtcagat
     2941 gcaaatcggg gaggatgtcg agctcgccgg gatcgattcc cagatcaaca ggttcctcgg
     3001 tttcaccggc gtcctgaccg cctggatcgt tctgattgtc cggcacgttc agatccggac
     3061 gcggaccgtg atcggggact tcaacatcgt cgccgatgct gtcggacgcc acatcggttc
     3121 cgccaaggtc atcttccggt tccgacccac ctccacaacc gggaacagcc gccatcaccg
     3181 caacaaggcc aaggcacgac aacatccttc cccaagacaa tgtattcatt gcatatcccg
     3241 aaatggttga cgcaggctag cgtacaggaa gccttaactt tgtcaacaaa atcaaggtcg
     3301 aaggctgtgt taacggcagg tttcgcgaca ggtcatggaa tgacgaaatc cttgaccacg
     3361 gccatatgct gcatctggga agaggacgaa tcgccggtct gtgccggcgg gaagtactcg
     3421 ttcagctcgg attgcgaaaa ggtccgcgta tcaaagacca ggccttccgg atagaccctg
     3481 tcagggccgg tcgaattgga ttgatacctg acgccgacct gatatccggc cattttctga
     3541 tccgccagca cgaaatcgta ctgcttgctg cggctggcgt tcgtattcac gttgccgtcg
     3601 tcgtcggccg gatccggcgg atcgaccatg aacacgccgt cctgcccgaa accgctttca
     3661 gacacggcgg aggtcccatt gaagtcgccg ccgactacgt acaggtactt gccctggttc
     3721 tgcgcgcgat gggtcttcac cttgccgacg atgacctggg cggcctcgac ctgatcggat
     3781 gagggactgg tatgcagatg aacgcttatc gcaaagatat cgaccggccc ggggatatca
     3841 atggtcgccc acatcaattc acggtttgac aggttcgggt cgtcccacca cccgcttccg
     3901 gtgatgggcc agcgactcag gacgccgttc ggtatctgga aacccgcgtc gtcaaccgcc
     3961 caataggacg ttccgacgac atcctgggcc agttcgttgt aatccgaggc ggtgttatcg
     4021 ccgtagttca tttcctggat gaggacgatg tccggccgaa gggcctgcat gatcctgatc
     4081 ccgtgaccat aatcgtaaga ctggtaattg ccgctggtga cattcgcggc gactatcctg
     4141 accggaagac ctggcgtcga gatgtcggac ccgccattat cgacagtcac tggaacgtca
     4201 tcggaaacat ccggaccgtc gtcggccaca tgctggtcgc gttccggcac gtcttcgaaa
     4261 atgtcgtcaa acacttcctc aaggatttcc gaccaggcat cggccagggc atcctcgatg
     4321 aatccatcgg gatcgccggc gtccgggccc gtgtccccgg ctgcgtccgg atcggcgtcg
     4381 ggaaggtcgg cgtctggatc gacattgccg tcaatcgcgg agtcagccag cgcatcaacc
     4441 gccacttcgt tcgcgacatc ggtcggcaaa tcggaagcgt cggacgcgtc cgccgtggaa
     4501 tcgacgttcg cctggtcacg cggatcggcg tcaccgggat ccgacacgtc attgtcaatc
     4561 aattcgacgg cggcatccgg ggaatcgacg gtatctgtgc caccgtttgc cccacagccg
     4621 ccggcgacga cggcaagcat catcaaagga atgattcgca tgagtgaaga atgatgattc
     4681 attgaggagt ccagaaagcc agcgccaaga ttacagatga aagccgcatg atgcaaggtc
     4741 ggcaggccac gattgaacag ttgaggatcg gacggcaaat gcccctggca ccgatggccg
     4801 atggattggc cgccaccgaa tcagcccgcc tttcgacgga tcgcccgacc tacctgtaga
     4861 aatggaacac gggctggact tcctggcacg ggcggcacat cgaggacggc aggccggtat
     4921 tgcgacgatt ccgaccggca acggccttga acaccggccc ggacttgacc tcgtcgcaga
     4981 caaggcattc gttcaggctg ccgtccggat tgtggtacgg ctgatccagt gcggcaatgc
     5041 acggcgcgaa gcattcctgc tgggtatgaa gcgtgttgta ataccagatc tgcgcgcatg
     5101 gaagctcgaa gcccagatcc atcaggcact ggatattgcc gtccttgttg ccaaaattgt
     5161 ccaggccgca ctggcgcacc ggatcggtca aatccggctg ccgcatatag acaacgagat
     5221 ttttcagagt ggaacagacg ccgcatccat cccagtgggt tatgaatgcc ccggcctcct
     5281 gcgcggcggc ggcgtcctga aaggtttcaa ggcgataacc gccggcaacg ctgtcatcaa
     5341 ggaacaggcc gcaaacggtt cccggaaccg gctggtgggc gtccggatcc tcccacgggt
     5401 cctcggtgac ctccggtgga gggttgacgg gaaccatcgc cagc
//
LOCUS       DAOPPA010000189         5427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16504_length_5427_cov_3.632725, whole genome shotgun sequence.
ACCESSION   DAOPPA010000189 DAOPPA010000000
VERSION     DAOPPA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16504_length_5427_cov_3.632725"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKH54_06935"
     CDS             <1..771
                     /locus_tag="PKH54_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99661.1"
                     /translation="VLLAGAGLLITARSPVKWLLLPVVAVLLPKLHVAASGTEFNHWF
                     RLFSMDVPLVMLLATVGLGRAIRFTSTWTAHRFWKLAAAAAIASSVLLPIRTAGNPAG
                     YVLRPELRDGGIRLRNPQIEVNYLIKLQNEHPECLFQTTVFDHGSFKDAWFGKNLKVT
                     LDRPTAWSGCTMFYLPLDCNFRDGPDCSESIKGLEEVESITFENAPYGQVAPYLDRHH
                     RNTVTLGLYRRHQVPGDGGTSTSGERASHSENEASSAL"
     gene            complement(690..2255)
                     /locus_tag="PKH54_06940"
     CDS             complement(690..2255)
                     /locus_tag="PKH54_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC99662.1"
                     /translation="MSGENPVKGEVGTVECRRAIVRLAIIAAVFVAAAGLTYLIPAAE
                     ALRPWVPGDDAPMKRLYTFDWSKGISGSGTESDGALKGEMARVSLAEGLGEELAANLG
                     SEAGVDQPVANRAADSSASQPSLISPDELVGLVRKIEDPSGKALDAFFRALQASASGR
                     SVTRIAHWGDSTIAADDVTGTLRRRLQSRFGDAGHGFMLVGKGTMPYRHKDIETVQLG
                     QWKQQMGIRRERRDGRYGYGGVAFTTWGGGSAWYKTVDKGPIGTSASSFEVFFQSHPA
                     GGKVNLAVDGKPAGTIDTRAGEVADGFRKIQVVSGPHRFDIDTEGEVRLYGVAIEDDG
                     PGVVYDSLGMVGARAQRFQNFDPAHFAAQVAHRAPDLLVIAFGGNECGDAMMDFDIYR
                     ENLRKTIDIVMAGRPTASCMLLAPLDQGEVGPRGRIRTMPAIPLIVQAQREVAAEKGC
                     AFFDTFSAMGGEGSMARWYKSRPRLGWGDYRHATPAGYEVIGNMIYKALLASFSEWLA
                     RSPEVEVPPSPGT"
     gene            complement(2269..3690)
                     /locus_tag="PKH54_06945"
     CDS             complement(2269..3690)
                     /locus_tag="PKH54_06945"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOC99663.1"
                     /translation="MLFHSLDYILFLFLAVTLFWALSRKDTLRILVLFLMSCRFYMVW
                     NPVYIFLVISSALIDYSAGRMIPLARTERGRKAWLILSIVTNLGVLITFKYYNFFYSV
                     VQDSLGLFGISVPAAYLKVLLPVGISFYTFQSMCYTIDVFQKKLEPVKNFLHFATFTV
                     FFPSLVAGPITKAQQLIPQLQARPRLTAELVGNGTFLIVKGLVKKIVFADFLALNLVD
                     RVFDNPQAFTSVETLIGLYGYTLQIYCDFSGYTDVARGSAMLLGIELPENFNRPYMAT
                     SPAQFWRRWHMTLSSWLRNYVYYPLGGSHCSTARTYWNLWLTLFLIGIWHGAGWTFVV
                     YGTIHGLAMVVHRYFYKKSGRTKTTVDPRWLEIIKIFVTFHFVVLSRILFRSPDLGVA
                     GDVASQLFAGSLSTAQVTWRTWLVLGVGFGIHWTPKSWVDAVQARYRALPAPVQGLVL
                     AGVAAVLFKLGSSQVVPYIYFQF"
     gene            3831..4850
                     /gene="galE"
                     /locus_tag="PKH54_06950"
     CDS             3831..4850
                     /gene="galE"
                     /locus_tag="PKH54_06950"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOC99664.1"
                     /translation="MRSVLVTGGAGYIGSHTCLEMARSGIDVVVVDNLCNSRMEAVRR
                     VEGISGKGITFYRADICDEAAMERIFERHEFSSVVHFAGLKAVGESVAKPLEYFHNNI
                     TGTVVLLRAMLRHEVRNMVFSSSATVYGDPQTVPVREDFPLSVVNPYGRTKLVIEDMM
                     RDLHRSDDRWNIMLLRYFNPVGAHESGQIGEDPEGVPCNLMPYITKVALGSLPELKIF
                     GGDYPTRDGTGVRDYIHVVDLAVGHLAALRKLEENPGCVAYNLGTGRGCSVLEMVNAM
                     SRACGRAIPYSIVDRRPGDAAEVWCDPSRAMMDLGWKAERDLDAMCADAWRWQQKNPD
                     GYPKD"
     gene            complement(4852..>5427)
                     /locus_tag="PKH54_06955"
     CDS             complement(4852..>5427)
                     /locus_tag="PKH54_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99665.1"
                     /translation="DFMELFQLIHSKGMKVVGLSLSPWGSDKDKRWVGIDAVRNQDRT
                     RQCVDWMLGRLTPGEALGKYAAGKKSWDPADLPDVAIDLYDSPLRDVNARPRPAADIK
                     ARMARDKQLNRELSALPQQSRDARYQKIMKQAVEMPKWFLRPELQSFDHIHPNREGHR
                     IIVETACPKLPAEWGCDCTYIRNAGKKAASR"
BASE COUNT         1164 a         1639 c         1602 g         1022 t
ORIGIN      
        1 gtcctgcttg cgggagccgg acttctgata acggcccgca gtcccgtcaa atggcttctg
       61 ctgccagtcg ttgccgttct ccttcccaag cttcacgttg ctgcgtccgg aaccgaattc
      121 aaccactggt tccgactgtt ttcgatggac gtcccgcttg tcatgctgct tgccaccgtt
      181 ggccttggcc gcgccatccg attcacgtcg acatggacgg cgcaccgatt ctggaagctc
      241 gccgcggccg ctgccattgc gtcatcggtt ctgcttccga taaggacggc cggaaatccg
      301 gccggttacg tgctgcggcc ggaacttcgg gacggcggca tcaggctgcg aaacccgcag
      361 attgaggtca actatctgat caagctccag aatgagcatc cggaatgcct ttttcaaacg
      421 accgtgttcg accacggctc attcaaggac gcctggttcg gcaagaatct gaaggtcacc
      481 ctggatcgac cgaccgcatg gagcggctgc accatgttct atctgccgct ggactgcaat
      541 ttcagggatg gtccggattg cagcgaatcg atcaaagggc tggaagaggt tgagtcaatc
      601 acgttcgaga acgccccata tgggcaggta gctccgtacc tcgaccggca ccacagaaac
      661 accgtcaccc tcgggctgta tcgcaggcat caggttcccg gcgatggcgg gacttccact
      721 tccggggagc gcgccagcca ttcggagaac gaagccagca gcgccttgta gatcatgttg
      781 ccgatgacct cgtagccggc cggagtggcg tgacgatagt cgccccatcc caggcgcggc
      841 ctggacttgt accagcgcgc catggatccc tcgccgccca tcgccgagaa ggtgtcgaag
      901 aacgcgcaac ccttttccgc cgcgacctcg cgctgggcct ggacaatcaa tggaatcgcc
      961 ggcatggtcc gaatcctgcc gcgcgggccg acctcaccct gatcgagtgg cgccaggagc
     1021 atgcaggatg ccgtcggacg accggccatg acgatatcaa tcgtcttgcg cagattttcg
     1081 cgatagatat caaaatccat catggcatcg ccgcactcgt ttcctccgaa cgcgattaca
     1141 agcaaatccg gggctcgatg cgccacctgt gccgcgaagt gggccgggtc aaaattctga
     1201 aaacgctggg ccctggcacc caccattcca agcgaatcat agacgacgcc gggcccatca
     1261 tcttcaatcg cgacaccgta aagccttacc tcgccttccg tgtcgatatc gaacctgtgc
     1321 ggaccggaga cgacctgaat cttccggaat ccgtcggcaa cctccccggc cctggtgtca
     1381 atcgttccgg caggcttgcc atcgactgcc agattgacct tcccaccggc gggatgggac
     1441 tggaagaaaa cctcgaagga agatgcgctg gtgccaatcg ggcccttgtc aaccgtcttg
     1501 taccaggcgc tgccgccacc ccaggtcgtg aaagcgaccc cgccgtagcc gtagcggccg
     1561 tccctgcgtt cccgacggat gcccatctgc tgcttccact gaccaagttg cacggtctcg
     1621 atgtccttgt gtcggtaggg catggtgccc ttgccgacca gcatgaatcc atgacctgca
     1681 tccccgaacc tggactgcag acgccgacgc aacgttcccg tcacgtcgtc agccgcgatg
     1741 gtcgagtcgc cccagtgggc aatccttgtg accgaccgac cggatgcgga agcctgaagc
     1801 gcgcggaaaa acgcatccag cgcctttccc gacggatcct cgatcttcct caccaggccg
     1861 accagctcat cgggtgatat cagggatggc tgcgatgcgg acgaatccgc ggcccgattc
     1921 gccacgggct gatccacgcc ggcctccgaa ccaaggttgg cggccagttc ctctccaagg
     1981 ccttccgcca gtgacaccct ggccatttcg cccttcagcg cgccgtcgga ctcggttccg
     2041 ctgcccgaaa tccctttgga ccagtcgaag gtgtaaagcc gcttcatcgg ggcatcatcg
     2101 ccgggaaccc atggtcgaag ggcctcggcg gccgggatca ggtatgtaag gccggctgcc
     2161 gccacgaaca cggcagctat aatggcaaga cgaacgatcg cgcgcctgca ttcgaccgtc
     2221 ccgacttcac ccttgaccgg attttccccg gacaccgtca ccaccccgtc agaactggaa
     2281 atagatatat ggcacgacct ggctggaacc gagcttgaac agcacggcag ccaccccggc
     2341 cagcaccagg ccctgaaccg gggccggaag cgcccggtac cttgcctgaa ccgcatcgac
     2401 ccacgacttc ggggtccagt gaatgccaaa gcccacaccg agtaccagcc aggtgcgcca
     2461 tgtaacctgg gcggtcgaaa gggatccggc gaaaagctgc gacgccacat cgccggcgac
     2521 gcccagatcc ggcgatctga acagtatcct ggacagaacc acaaaatgga aagtcacgaa
     2581 tatcttaatg atttcaagcc atctgggatc gaccgtggtc ttcgtccggc cgctcttttt
     2641 atagaagtag cggtggacga ccatggcaag gccatgaatg gttccgtaaa ccacgaacgt
     2701 ccagccggcc ccatgccaga tgccgatgag gaagagtgtc agccagaggt tccagtatgt
     2761 ccgggcggtt gaacagtgtg aaccgccaag agggtaatag acgtagttgc gcagccacga
     2821 actcagggtc atgtgccagc gtcgccagaa ctgcgcggga ctggtggcca tgtagggacg
     2881 gttgaaattc tcgggaagct cgatgcccag gagcatggcc gaaccacggg ccacgtcggt
     2941 gtaacctgaa aaatcgcaat agatctgaag cgtgtagccg taaagaccga tcagcgtctc
     3001 gaccgacgtg aaggcctgcg ggttgtcaaa cacacggtca acaagattca gggccaggaa
     3061 gtctgcgaag acgatcttct tcacaagccc cttgacgatc aggaaggtcc cgttcccgac
     3121 cagttccgcg gtcagccggg gacgggcctg aagttgggga atcaactgct gggccttggt
     3181 gatcggcccg gcgacgagtg aagggaaaaa cacggtgaaa gtggcgaagt gaaggaaatt
     3241 cttgaccggc tcaagcttct tctggaagac gtcgatggtg tagcacatcg actggaatgt
     3301 gtagaacgat atgcccaccg gcagcagaac cttcaggtaa gccgccggca cgcttatgcc
     3361 gaacagcccc agggagtcct ggaccacgga atagaagaag ttgtagtact tgaaggttat
     3421 caggacgcca aggttggtca caattgacag gatcagccac gccttgcgcc cccgctcggt
     3481 cctggccagt ggaatcatgc ggccggccga gtaatcgata agggccgaag aaatcacaag
     3541 aaagatatag accgggttcc agaccatgta gaaccggcat gacatcagga aaagcacaag
     3601 gatgcgcaag gtgtcctttc gagaaagtgc ccagaacagg gtcaccgcca ggaacaggaa
     3661 caggatgtaa tcaagactgt ggaaaagcat ctgcccgaat cttccccagg gttccggaac
     3721 ctggcccggc tccggtgatg gcggattgta tattcaaaag caccttcaat ccagctaata
     3781 tcgcgcacgt caacgggaat cgcggccgga agccgccagg aggttgccgc atgagatcag
     3841 tcctcgtaac cggtggcgcg ggatacatcg gaagccacac gtgcctggag atggcgcgtt
     3901 ctggaattga cgtcgtcgtg gtggataacc tttgcaacag ccggatggaa gcagtccggc
     3961 gggtcgaggg catttccgga aaagggatca ctttctaccg ggccgacatc tgcgatgaag
     4021 ccgccatgga aaggatcttc gagaggcacg aattttcatc agtggtgcat ttcgccggct
     4081 tgaaggcggt cggggaatcg gtcgcgaagc cgcttgaata cttccataac aacatcaccg
     4141 ggaccgtggt gctgcttcgc gccatgctac gtcacgaagt aaggaacatg gtcttttcct
     4201 cctcggctac ggtgtatgga gacccgcaaa ccgtgcccgt gcgcgaggat tttccgctgt
     4261 cggtggtgaa cccgtacggc aggacgaagc tggtcatcga ggacatgatg cgcgaccttc
     4321 accgttccga cgaccgctgg aacatcatgc tgctgaggta tttcaatcct gtcggggccc
     4381 atgaaagcgg acagataggc gaggatcccg aaggcgtgcc gtgcaacctg atgccatata
     4441 tcacgaaggt cgcccttggc agcctgccgg aactgaagat attcgggggc gactacccta
     4501 ccagggacgg aaccggcgtc agggattaca tccacgtggt cgatcttgcg gtcggccatc
     4561 ttgccgccct gaggaaactc gaagagaacc ccggctgcgt cgcgtacaac ctggggacgg
     4621 ggcgcggatg ctcggtgctg gaaatggtca atgccatgtc gcgggcgtgc ggacgggcaa
     4681 ttccttattc catcgtcgac aggcgcccgg gagacgcggc cgaggtgtgg tgtgaccctt
     4741 ccagggcaat gatggacctc ggctggaagg ccgaacgcga tcttgatgcg atgtgcgcgg
     4801 atgcatggcg ctggcagcag aagaaccccg acggctatcc gaaggattga actaccttga
     4861 tgcggccttc ttcccggcgt tcctgatata cgtgcagtca cagccccact cggcgggcag
     4921 tttcggacac gcggtctcga cgataatccg gtgaccttcg cggttggggt ggatgtggtc
     4981 aaatgactgc aattccgggc gaaggaacca tttcggcatc tccaccgcct gcttcatgat
     5041 cttctggtat ctggcatccc tggactgctg gggaagagcc gacagttccc tgttcaattg
     5101 cttgtccctg gccattctgg ccttgatgtc agcggccggg cgcggccggg cgttgacgtc
     5161 ccgaagcggt gaatcgtaca gatcaatggc cacgtccggc agatccgccg gatcccatga
     5221 tttcttcccc gccgcgtact tcccaagagc ctcgcccggg gtcaggcgcc ccagcatcca
     5281 gtcaacgcac tgtctggttc tatcctgatt cctgacggca tcgatgccga cccagcgctt
     5341 gtccttgtcg gaaccccagg gagaaaggga aagacccacg accttcatgc cctttgagtg
     5401 gatgagctgg aaaagttcca tgaagtc
//
LOCUS       DAOPPA010000190         5408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16572_length_5408_cov_3.484214, whole genome shotgun sequence.
ACCESSION   DAOPPA010000190 DAOPPA010000000
VERSION     DAOPPA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_5408_cov_3.484214"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKH54_06960"
     CDS             complement(<1..676)
                     /locus_tag="PKH54_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99666.1"
                     /translation="MITRIFHESAFGRGAIAAAFIMILSSHAVAQEVEPEAQPEAVGI
                     EEPVPATEQAVEPADEAVVESAGEPASSPDPEAAAVAEPGDSAGTPEKPEEPKKKWNV
                     GKYVQFHGLLQTVIVYRNDYDFDSTPRYYDVEGQTVGMLGTFFKPQISITPDKHLRIF
                     WEMELGLNLWSRHNPDQYTSGNFDTFRLAHRELYVEGKFFEKKLNFKVGYQFFEDPTR
                     LFLGHWL"
     gene            complement(692..3271)
                     /locus_tag="PKH54_06965"
     CDS             complement(692..3271)
                     /locus_tag="PKH54_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOC99667.1"
                     /translation="MKKFRTGSKWRIGAVAVPVVAVLAGMLAAGCSSDEPAFDKSVSR
                     IVTERTWFKDQEGRYVHFRGINVSGDAKLPVMLDVPLDNGRDFTFVGRPFPLDEADKW
                     FGQIRALGFNSVRLLFLWESVFPDDRNTPDKDFLDYFEAIISKANDHGIYVLINMHEN
                     LWGRDMVAKFNDQAPGVRGDIENMLYSLLPPHNNQVVGDGAPTWATKACMPYKKFDSP
                     NWGTTYLLGAMAKDNDGIPGSDFLYTVELVAGILGFDLPAGLVEEIGAKLPAQPFDRS
                     ETAGMLPLSGWWDNMIFSIDIQRCYAAMFAGDSVFPDVRIEPDGTVKNINDDEVSEDA
                     MDMKEYFQGSLERAWVEVAKRAVKYPNIIGYDLMNEPAASLYVFAALALFFEVGNEAA
                     VKEVLNLLLPNELLVNGSPLAIDLPFEPGDNCDDSCANTCGTVCNTDCNAQCGTDCDA
                     MCGADDNCKAVCQEQKSNCTEQCNTQKTACNKLCSNQVATCIDLCRDPARDQLGTKVF
                     YLLKFLEVLPPDAEWETRVKWGYGDVDLFGALGVLDAFDYFLLQPYFERVGNAIQKVD
                     ENAIIWLEPTGSGGINGTFMTKPAGIKQAVYAPHYYPDIYPWLGFNMPERDFSVDEIQ
                     YRDYTNDLIASASSSKESLGNIPVVFGEFGTYWNYKYKGEDWEEKGILQSIRNNYSLS
                     AHFLDNYYESYEKMFVSSFLWCYSIRNTYENGEGWNKEDFSIIGPDQKPRGELAWSRP
                     YPRALSGKPVAMHFYSDYHYYDPEKGIPDPRREFELVFSSKETDAPTIIYVPNAQYPD
                     GFHVWLSDGWMAWDQADQVMYYYPTNDLPEAEHKVIIRPPFEEWTTEGWKYFVKGGQV
                     ISR"
     gene            complement(3329..4930)
                     /locus_tag="PKH54_06970"
     CDS             complement(3329..4930)
                     /locus_tag="PKH54_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOC99668.1"
                     /translation="MRSVLIAIAFLAVTTGCGDDNQHSGDIPGDDVMMDSGSDTTSPD
                     VVPDVAGDTAPDVVESGMSRLRAEGMNIVDEDGNVVVLKGVNLGGYLYHETWLTLVDY
                     SAQARAWKVAVEMELEDIVRPVLVAIGPQIVAGMVGVSDGSGGSIGADAWLELLRTEL
                     GEVADTATVEAYFTELEKYLPAIYDDSDLPIYTKLEERFGIEARDSLLDTYQGAWIQE
                     RDIEWLAGQGFNLVRVPIGYRTLTKGSHLAQPTELVWNEDAFARIHALLDWCEKWGVY
                     AVLDIQECPGGQNDYSGPALLYSTQMYQDMTVDLWEEMSARFHDRNVVAAYSLLAEPM
                     GAPSRKARDDMYDKLVKAIRALGDDHLLVIHDGFKGMDGMADPAEYGWTNVVFSTHIF
                     EWSARNFDTFKGTVDLYDFFFRTAQETRNVPYFIGSFSGIFDEPWAYEAVEYIVEWMG
                     KYGWSWSLWAYKDVQDPVAADLWGDKSSWGVKSRLVGEFDRPDLFIDSEETVRQKFAS
                     YGSLELEVNQTLLDVLKADLQSQPE"
     gene            complement(4935..>5408)
                     /locus_tag="PKH54_06975"
     CDS             complement(4935..>5408)
                     /locus_tag="PKH54_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99669.1"
                     /translation="DSGGVREWLGHGETGLLVLKALLQKASGGNAAAYLEQAAVSMYE
                     SNSCRFTFGEGEIEDLLEAASKAVVKVGTGRLVEVAFDVPPCTAGSEWSVPVSAMDSS
                     VSVFGLPGATVREGAIGWLPPHAGMFAGVVIAVAADGLGFMEFEVVCEDPGRGKE"
BASE COUNT         1054 a         1697 c         1531 g         1126 t
ORIGIN      
        1 caagccagtg acccaggaaa agcctggtgg gatcctcaaa gaactggtaa ccgaccttga
       61 aattcaactt cttttcaaag aacttgcctt caacgtacag ttcacggtgc gcaaggcgga
      121 aagtgtcgaa gtttccgctg gtgtactgat ccgggttgtg acgactccag aggttcaggc
      181 caagctccat ttcccagaag atgcgcagat gcttgtccgg cgtgatggaa atctgtggct
      241 tgaagaacgt tcccagcatg ccgacggtct ggccctcgac gtcgtagtac cggggcgtcg
      301 aatcgaagtc gtagtcgttc cgatagacga ttaccgtctg cagaaggcca tggaactgga
      361 cgtacttgcc cacgttccat ttcttcttcg gttcttccgg cttttccggc gtcccggccg
      421 aatcgcctgg ttccgccacg gcagccgcct ccggatccgg ggaagaagcc ggttcgccgg
      481 cagattcaac aactgcttcg tctgccggtt cgaccgcctg ttccgttgcg ggtacaggtt
      541 cctcgatccc gaccgcttcc ggctgggcct cgggttccac ttcctgcgcc actgcatgcg
      601 acgacaggat catgatgaat gctgctgcaa ttgctccacg gccaaaagcc gattcatgga
      661 aaatgcgcgt gatcatgacg attcctccag attatcggct tattacctgg ccgcccttga
      721 cgaagtactt ccagccctcg gtggtccact cctcgaacgg aggcctgata atcaccttgt
      781 gctcggcttc cggaaggtcg ttcgtcggat agtagtacat cacctggtca gcctgatccc
      841 aggccatcca tccgtccgac agccacacat ggaatccgtc gggatactgc gcgttcggga
      901 catagatgat cgtcggcgcg tcggtttcct tgcttgaaaa caccagttcg aattcgcgcc
      961 ttggatcggg aatgcccttt tccgggtcgt agtagtggta atccgagtag aagtgcatgg
     1021 caaccggctt gccggacagc gccctcggat acggcctgga ccacgccagt tcgccgcgcg
     1081 gcttctggtc gggaccgatg atgctgaagt cttccttgtt ccagccctcg ccgttttcgt
     1141 aagtgttgcg gatcgagtag caccacagga agctcgacac gaacatcttc tcgtacgact
     1201 cgtagtagtt atccaggaag tgcgccgaca gcgagtaatt gttgcggatc gactggagga
     1261 tgcccttttc ctcccagtcc tcacccttgt acttgtaatt ccagtaggtg ccgaattcac
     1321 cgaagacgac cggaatgttc ccgagcgact ccttcgaaga ggaagccgac gcaatcaggt
     1381 cgttggtgta gtcgcggtac tgaatctcgt ccaccgagaa atcgcgttca ggcatgttga
     1441 acccgagcca cgggtagatg tccgggtagt agtgaggcgc atagacggcc tgcttgatgc
     1501 cggccggctt cgtcatgaac gtgccgttga tgccgcccga tccggtcggc tcgagccaga
     1561 tgatcgcgtt ctcatcgacc ttctggatcg cgttcccgac gcgctcgaag tacggctgca
     1621 gcaggaagta gtcgaatgca tccaggacgc ccagggcgcc gaaaagatcg acgtcgccat
     1681 agccccactt gaccctggtt tcccattcgg cgtccggcgg aagaacttcc aggaacttca
     1741 gaagatagaa gaccttggtt ccaagctgat cccgggccgg atcacggcac agatcgatgc
     1801 aggtcgcgac ctggttcgaa cacagcttgt tgcaggccgt cttctgggtg ttgcactgct
     1861 cggtgcagtt ggacttctgc tcctggcata ccgccttgca gttgtcgtcg gcgccgcaca
     1921 tggcatcgca gtcggttccg cactgtgcgt tgcagtccgt attgcagacc gttccgcagg
     1981 tgttggcgca ggaatcgtcg cagttgtcgc ccggctcgaa cggcaaatcg attgccagcg
     2041 gggatccgtt cacaagcagt tcgttcggga gcagcagatt caggacttcc ttgaccgcag
     2101 cttcattgcc gacttcgaag aacagggcaa gggcggcaaa gacataaagc gaggcggccg
     2161 gttcgttcat caggtcgtaa ccgatgatgt tcggatactt gacagccctc ttcgccacct
     2221 cgacccaggc ccgctcgagc gacccctgga agtactcctt catgtccatc gcgtcttcgc
     2281 tgacttcgtc gtcgttgatg ttcttgacag tgccatccgg ctcgatcctg acatccggaa
     2341 acaccgagtc gcctgcgaac atggcggcat agcaacgctg gatgtcgatg ctgaagatca
     2401 tgttgtccca ccagccggac agcggcagca tgccggccgt ctcggaccgg tcgaacggct
     2461 gggccggcag cttggcgccg atttcctcga caagtcccgc tggcaggtcg aatcccagga
     2521 tcccggcgac cagttcgacg gtgtagagga aatcgctgcc cggaatgccg tcattatcct
     2581 tggccattgc gccaagcagg taagtagtgc cccagttcgg gctgtcgaac ttcttgtaag
     2641 gcatgcacgc cttcgtcgcc caggttggcg cgccgtctcc aactacctgg ttgttgtgcg
     2701 gcggcagcag cgagtacagc atgttttcga tgtccccgcg gacgccgggc gcctggtcat
     2761 tgaacttggc gaccatgtcg cgcccccaca ggttctcgtg catgttgata agcacgtata
     2821 tgccgtggtc gttggccttg ctgatgattg cctcgaagta gtccagaaag tccttgtccg
     2881 gagtgttcct gtcatccggg aagaccgact cccacaggaa aagcagacgg acggagttga
     2941 aaccgagcgc acggatctga ccaaaccact tgtctgcctc gtccagcggg aacggacggc
     3001 cgacgaacgt gaaatcgcgg ccgttgtcca gcggtacgtc aagcatcacg ggcagtttgg
     3061 catcgccgct gacgttgatg ccgcggaaat ggacatagcg gccttcctga tccttgaacc
     3121 aggtccgctc ggtgacgatg cgcgaaaccg acttgtcgaa agccggctcg tcgctggaac
     3181 acccggccgc cagcatgccg gcaagcacag ccacgacggg caccgccacg gcgccaatcc
     3241 gccatttcga tcctgtccgg aacttcttca tggtcttcct ccggaatcgt tttcaatgcc
     3301 cccgcccaaa cgggcgggac ggctccaatc actcgggttg cgactgcaaa tcagccttga
     3361 gtacgtccag cagggtctga ttgacttcca gctcaagcga accgtaggac gcgaacttct
     3421 gcctgacggt ctcctcgctg tcgataaaca ggtcgggacg gtcgaactcg ccgacaagac
     3481 ggctcttcac gccccagctt gacttgtcgc cccagagatc cgcggccacc ggatcctgga
     3541 cgtccttata agcccacagc gaccaggacc agccgtattt tcccatccac tcgacgatgt
     3601 attcgaccgc ctcgtaagcc cacggctcat cgaagatgcc cgagaagctg ccgatgaaat
     3661 acggtacgtt cctggtctcc tgggcggtgc ggaagaagaa gtcgtaaaga tcgaccgtgc
     3721 ccttgaatgt gtcaaaattc ctggccgacc attcgaagat gtgggtcgag aacacgacgt
     3781 tggtccatcc gtattcggca gggtcggcca ttccgtccat tcccttgaag ccgtcgtgga
     3841 tcaccagcag gtgatcgtcg ccaagcgcgc gaatggcctt gaccagcttg tcgtacatat
     3901 cgtcgcgggc cttgcgcgac ggggcgccca tcggttctgc aagcaggctg tatgccgcca
     3961 cgacgttcct gtcgtggaac ctggcggaca tctcttccca cagatccacc gtcatgtcct
     4021 ggtacatctg ggttgaataa agcagcgccg ggcccgagta atcgttctgt ccgccagggc
     4081 attcctggat gtccagaacc gcatagactc cccacttctc gcaccagtcc agcaaggcat
     4141 ggatccgtgc gaacgcatcc tcgttccaga ccagttccgt cggctgcgcc aggtggctgc
     4201 ccttcgtcag cgtgcggtat ccaatcggca cgcgcaccag gttgaacccc tgcccggcga
     4261 gccactcgat gtccctttcc tgaatccagg cgccctgata ggtgtccagc agggagtccc
     4321 tggcctcgat tccgaaacgt tcctcaagct tggtgtagat cggcagatcc gagtcgtcgt
     4381 atatcgcggg caggtacttt tccagttccg tgaaataggc ttcaaccgtg gccgtgtcgg
     4441 caacttcgcc aagctcggtg cgcagcagtt ccagccatgc gtcagcacct atgctgccac
     4501 cgctgccatc gctgacgccg accattccgg cgacgatctg cggaccgatc gcgacaagta
     4561 ccggtcgaac gatatcctcg agttccatct ccacggcgac cttccaggca cgcgcctggg
     4621 ccgagtaatc gacaagtgtc agccacgttt catggtacag gtatccgcca aggttcacgc
     4681 ctttcaggac aacgacgttt ccgtcctcgt caacgatgtt catcccttcg gcgcgaaggc
     4741 gcgacatgcc ggattcgacc acgtccggtg cggtgtcgcc cgcgacatcc gggacaacgt
     4801 cgggggaagt cgtatccgaa cccgagtcca tcatgacgtc gtcgccgggg atgtccccgc
     4861 tgtgctggtt gtcgtcgccg caaccggtcg tcacggccag gaaagcgatg gcaatcagta
     4921 cactcctcat cttctcattc ctttcccctt ccggggtctt cgcacaccac ttcgaactcc
     4981 atgaagccga ggccgtcggc ggccacggca ataaccacgc ctgcaaacat acccgcgtgc
     5041 ggcggcagcc atccgatggc gccttccctc accgtcgctc cgggaagccc gaacaccgac
     5101 acggacgagt ccatggcgga caccggaact gaccactcgg aaccggcggt gcatgggggg
     5161 acatcgaaag cgacttcgac aaggcgcccg gttccgactt taacaacggc ctttgaagcg
     5221 gcctcgagca ggtcctcgat ttcgccctcg ccaaaggtga acctgcaact gttggactca
     5281 tacatggaaa cggcggcctg ctccaggtaa gcggcggcgt tgccgccgga cgccttctga
     5341 agcagcgcct tcagcaccag cagcccggtc tcgccgtgac cgagccactc gcgcaccccg
     5401 cccgagtc
//
LOCUS       DAOPPA010000191         5407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16576_length_5407_cov_3.358371, whole genome shotgun sequence.
ACCESSION   DAOPPA010000191 DAOPPA010000000
VERSION     DAOPPA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16576_length_5407_cov_3.358371"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /gene="mraY"
                     /locus_tag="PKH54_06980"
     CDS             complement(<1..189)
                     /gene="mraY"
                     /locus_tag="PKH54_06980"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOC99670.1"
                     /translation="MILYLSEILIQYLSSLNVLRYVSTRTMAAFFTSLAITFVFFPMF
                     IRFMERKRVKQPERPCVSE"
     gene            complement(190..1647)
                     /gene="murF"
                     /locus_tag="PKH54_06985"
     CDS             complement(190..1647)
                     /gene="murF"
                     /locus_tag="PKH54_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOC99671.1"
                     /translation="MTRTSKYELSGESIAEFTGGRLVSGAGLYATGGASIDTRTIRPG
                     QVFFALDGERSRGVRFVETAQERSAAGIVVSDADLQDPAVVRVIESGRVFVVAVPDTT
                     DALIAAARNWADILSPKVVAITGSVGKTTVKNLTSAAIGAARPTHWTTGNHNNRLGVS
                     LTCLGLRPSHEVLVAEMGMNAPGEIAELCTIVKPDVGLVTTVAPVHLEGLGSIDNVAA
                     AKSELIRSLGPGATAILNADDHLVAGMMTIAEENGARVVWFGSCEAADVRLLDAQADH
                     DGRVVARISVFGRECRVNLRLVGIHNAMNAAAALAAAAVCGVDPEEACRAMAAVEPGK
                     HRMELTDIGTIRLVDDCYNSSPKSVEAALEALASIAGPRRRVAVLGDMLEMGEHTDAI
                     HREMGRVAAEHQVNCLIAVGRNASTIAGAAVVGGIPRSEVFEAPDSLAAASLARELLK
                     PRDVVLVKASRGVGLELVVDALKARFGQGNTEAGN"
     gene            complement(1644..3149)
                     /locus_tag="PKH54_06990"
     CDS             complement(1644..3149)
                     /locus_tag="PKH54_06990"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOC99672.1"
                     /translation="MRIDGERLVSLTGAAIAFGNPSGLSVDSVTSDSRSVRPGCAFVA
                     IQGTRVDGHAFAAAAVQAGAAVIIGSRPLDPAVIGDVPELVVSDPRQALSVLAAELAG
                     NPTAGIELTGVVGTDGKTSTTLIIEAGLQGCGRTTGLLGTVQYRYAGKVIESSMTTPD
                     PVTLQDLFARMRDAGVDAAVMEVSSHAIDQRRVESCRFKYGVFTNLGRDHLDYHVTLD
                     AYREAKLRFFTEVLPSNPDAVGSVVNDDDPVSCMIRERSPLPVIGWTFGGCQGSEIRL
                     VKAEYSFDGTMFEVETPWGRFEVRTSLIGRHNVANIMAAIGVAGVAGLDIGRFIAGIA
                     ALTTIPGRLERVRPDAAVKVFVDYAHTPRAIESVLAILGGIDRGARLHVVCGAGGDRD
                     RGKRPLMGSAAVMNADVAWITSDNPRSEDPLDIIGQIVDGIRQAEADGGSRARWEVEP
                     DRREAIRKAISGARHGDVVLIAGKGHENYQILKDRTIHFSDVEVAGEMLER"
     gene            complement(3149..5029)
                     /locus_tag="PKH54_06995"
     CDS             complement(3149..5029)
                     /locus_tag="PKH54_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOC99673.1"
                     /translation="MGQGGMLQKGVETVMRGLTGKTDRIERRPVFRMAVVLGLLGLAM
                     VAVLIQAWRYQGPASQFLAAKARRQTTSSDSLSGNRGSIFDSSGRRILAVTVRNSTVT
                     STGGAWQDDWLMIANSLAVILEMPVEEVFRKVWDGIGKAVVIKKDVGDVEAAAIRRLN
                     LPSIVLISDEVRYYPLELIMGSVLGYLRPHESIRGKMVGELGVEQSYDELLSPQVRKF
                     PVGRDKDRQGFYEGSVGEGWFLDGSDLHLTIDARLQMILDNAVATAVRDEDAKGAMAV
                     MLDPRTFKILAMSSYPFLDPNDYKNACAGSDHPDDGSNPCRNKVVSYAFEPGSIAKVL
                     GLSAILESGQMNLGSSVNGGHGFCRVGGHDVRDVKGVGVVSLKEAVKVSSNCAMADGA
                     RHVQPSALHDVFVRFGIGGKTGIDLPNESSGTLRREWSHVDAQVGVYGYGYRTTLLTM
                     ATAVATIVNGGKKLSPRVVDRMESADGTVTHVPTTAPDQVVSESTARQVREALRAVVM
                     DEGGTGSRARPQGYSAAGKTGTARIPTPKDQPRKYHCSFVGYAPAEEPAFVMAITLID
                     PRKRESPTGGQVAAPVFARVAEKVLPLFGHKPAIAEKTSNHRQGSVGTREGGDVQQEA
                     GN"
     gene            complement(5042..>5407)
                     /locus_tag="PKH54_07000"
     CDS             complement(5042..>5407)
                     /locus_tag="PKH54_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99674.1"
                     /translation="PTPYLAHANIGRAYMEMGDLESARKAYDMAVFLNPKFCLGYLNL
                     GHVFLKKHMVRDAQENYEKVTRFCPDFGESYYHLGVIHQKAGRTTEAMEAFEKCASLL
                     KDSPTGKRCMARAAGGQAE"
BASE COUNT          995 a         1796 c         1561 g         1055 t
ORIGIN      
        1 ttccgacacg cacggacgct cgggctgctt caccctcttc ctttccatga agcggatgaa
       61 catcggaaag aacacgaacg tgatggccag cgacgtgaag aaggccgcca tcgtccttgt
      121 cgagacgtac cgaagcacgt tcagggaact caggtactga atcaggattt cagacagata
      181 aagaatcatc tagttccctg cctccgtatt gccctgcccg aacctggcct tcaacgcgtc
      241 aaccacgagt tccaggccga cacccctgga tgccttaaca agcacgacgt cgcgcggctt
      301 cagcagttcc ctggccaggg aggccgcggc cagcgaatcg ggcgcctcga agacctcgga
      361 tctcgggatt ccgccgacca cggcggcccc ggcgattgtc gatgcgttcc ggcccaccgc
      421 aatcaggcag ttgacctggt gttcggcggc gaccctgccc atctcgcggt ggatcgcgtc
      481 ggtgtgttca cccatctcga gcatgtcgcc caggacggcc acgcgccgtc ttggcccggc
      541 gatcgaggcc agtgcctcaa gggccgcctc gaccgatttc ggcgacgagt tgtagcagtc
      601 gtccacaagg cggatggttc cgatgtcggt caattccatc ctgtgctttc caggctcaac
      661 cgcggccatc gcccggcagg cttcctccgg atccactcca catacggccg cggccgcaag
      721 cgcggcggcg gcgttcatgg cattgtggat tccaaccagc ctcaggttca ccctgcactc
      781 gcggccgaac acgcttatcc tggcaaccac cctgccgtcg tgatcggcct gcgcatcaag
      841 cagtcggacg tcggcggcct cgcaggaacc gaaccagacc actcttgcgc cgttctcttc
      901 agcaatcgtc atcattccgg cgaccaggtg atcgtccgcg ttcaggattg cggtggcgcc
      961 cggaccaagg cttcgaatca gttcggactt ggctgcggcc acgttatcga tgctgcccag
     1021 gccttcgaga tgaaccgggg ctaccgtcgt gaccaggccg acatcgggtt tgacgatcgt
     1081 gcaaagctcg gcgatttcac cgggggcgtt catgcccatc tcggcgacaa gcacttcgtg
     1141 ggaaggtcgc aggccaagac aggtcaggga caccccgagc cggttgttat ggttgccggt
     1201 cgtccagtgt gtcggacggg cagccccgat tgcggcggaa gtaagattct tgacggtggt
     1261 ctttccgacc gaaccggtaa tcgcgacaac ctttggagaa aggatgtcgg cccagttccg
     1321 ggccgccgct atcaaggcat cggtcgtgtc cggaaccgcc acgacgaaca cgcggccgga
     1381 ctcgatgacg cggaccacgg cgggatcctg cagatcggcg tcagaaacga cgattccggc
     1441 cgcgctcctt tcctgggcag tctccacgaa cctgacgccc ctgctccttt cgccgtcgag
     1501 tgcgaaaaac acctgtccgg gcctgattgt cctggtatcg atcgacgcgc ctccggtagc
     1561 gtacagtccc gcgcccgaca cgagccggcc gcccgtgaat tcggcaatcg actcgcccga
     1621 aagctcgtat ttcgaggttc tggtcatctt tccagcatct cccccgcgac ttccacatcg
     1681 ctgaagtgaa tggttctgtc cttcaggatc tggtagttct catgtccctt gcccgctatc
     1741 aggaccacgt cgccgtgcct ggcgccactt attgccttcc ggatcgcttc ccggcgatcc
     1801 ggctcgactt cccagcgcgc ccttgatccg ccatcagctt cggcctggcg gatgccgtca
     1861 actatctgcc cgatgatgtc caacgggtcc tcggatctcg gattgtcgga agtgatccag
     1921 gcaacatcgg cattcataac ggcggcgctg cccatcaaag gccgcttgcc cctgtccctg
     1981 tcgcccccgg ccccgcatac cacgtgaagg cgcgcacccc ggtcgatgcc gccaagaatg
     2041 gctagcacgc tctcgatggc cctgggtgta tgcgcgtaat cgacaaagac cttgaccgcg
     2101 gcatccgggc gcacgcgttc aagcctgccc ggaatggtcg taagcgcggc gatgccggcg
     2161 atgaaccgcc cgatgtcaag gccggcaact ccggcgacgc cgatggcggc catgatgttc
     2221 gcgacattgt ggcgaccgat cagtgacgtt cgcacttcga accgtcccca cggggtttca
     2281 acctcgaaca tggttccatc aaacgagtac tcggctttca cgagccggat ttcgctgcct
     2341 tgacagccgc cgaaagtcca tccgataacc ggaagcggac ttctttcacg aatcatgcag
     2401 gaaacgggat cgtcatcgtt tacaaccgat ccgacagcgt ccggattcga cgggagcacc
     2461 tcggtgaaga agcgcagctt ggcctcccgg tacgcatcaa gcgtcacgtg atagtcaaga
     2521 tggtcgcgcc cgaggttggt gaatacgccg tatttgaacc ggcaggactc aaccctccgc
     2581 tggtcgatcg cgtgcgacga cacttccatg acagcggcat cgacgccggc gtcccgcatc
     2641 ctggcaaaca gatcctgaag cgttacgggg tctggagtcg tcatgcttga ctcgatgacc
     2701 tttcccgcat agcggtactg cacggtgcca agaaggcctg tggtcctgcc gcatccctgc
     2761 aggccggcct cgatgatcag cgtcgtcgat gtcttgccgt ccgtcccgac caccccggtc
     2821 agttcaatcc cggctgtcgg gttgccagcc agttcggccg cgagcacgga aagcgcctgg
     2881 cgcgggtcgg acacgacaag ttcaggcacg tctccgataa cggccggatc caggggtctg
     2941 gaaccgataa tgaccgccgc gcctgcctgg acggcggcgg cggcaaaggc gtgtccatcg
     3001 accctggttc cctggattgc gacgaacgcg cagccggggc gcacggatcg cgagtcggaa
     3061 gtcaccgaat cgaccgacag gccgctcgga ttcccgaaag cgatggccgc gcccgtcagg
     3121 gaaaccagcc tttctccgtc aatccgcatc agtttccggc ctcctgctga acatctccgc
     3181 cttccctggt gccaacgctt ccctgcctgt gattggatgt cttttccgcg atggcgggct
     3241 tgtggccgaa aagcggcaga accttctcgg ccaccctggc gaagaccggc gcggcgacct
     3301 ggccaccggt cggggattcg cgctttcgcg ggtcaatcag cgttatcgcc atcacaaagg
     3361 ccggctcctc tgccggcgca tatccgacaa acgaacagtg gtatttcctg ggctgatcct
     3421 tcggggtcgg tatcctggcg gtgcctgtct tgcccgcggc ggagtaaccc tgcggcctgg
     3481 cccttgaacc ggtgccgcct tcgtccatga caaccgcgcg cagcgcttcc ctgacctgtc
     3541 gcgccgtgga ctccgagacg acctgatccg gggctgtcgt gggcacgtgc gttaccgttc
     3601 catcggcaga ctccatcctg tcaacgaccc ggggagagag cttctttccg ccgttgacta
     3661 tggtcgcgac agcggtcgcc atggtcagca gcgtggttcg atagccgtag ccatatactc
     3721 cgacctgggc atcgacatgg ctccactcgc gccggagcgt gcccgaagac tcgttcggca
     3781 ggtcgattcc ggtctttccg ccaatgccga acctgacgaa cacgtcatga agcgccgaag
     3841 gctgcacatg ccttgcgccg tcggccatcg cgcagtttga ggagaccttg accgcctcct
     3901 tgaggctgac cacgccgacg cccttgacgt cccttacatc gtgtccgccg acccggcaga
     3961 agccgtgacc tccgttaacc gaactgccaa ggttcatctg gccggattca agaatcgccg
     4021 acagtcccag aaccttggcg attgatccag gttcgaacgc atacgaaacg accttgtttc
     4081 tgcaggggtt cgaaccgtcg tccggatgat cgctgccggc gcaggcgttc ttgtagtcgt
     4141 tcggatccag gaacggatac gacgacatcg caagaatctt gaaagtccgc gggtcaagca
     4201 tgacggccat cgcgcccttt gcatcctcgt cgcggacggc ggttgcgacc gcgttgtcca
     4261 ggatcatctg aagacgggca tcgatcgtca ggtgaagatc cgagccgtca aggaaccagc
     4321 cctctccgac cgagccttca tagaatccct ggcgatcctt gtcccgcccg accgggaact
     4381 tcctgacctg gggactcagc agttcgtcgt aggactgctc gacgccgagt tctccaacca
     4441 tctttccgcg aatgctttca tggggcctca ggtagccaag aaccgacccc atgatcagtt
     4501 caagcgggta atacctgact tcgtcggaaa tcagcacaat cgagggcagg ttcaggcgcc
     4561 ggattgccgc ggcctcgaca tcgccgacgt ccttcttgat gacgacggcc ttgccgattc
     4621 catcccacac cttccggaac acttcctcga caggcatctc aaggatgacg gccagtgaat
     4681 tcgcgatcat cagccagtcg tcctgccatg ctcccccggt ggatgtcact gtggaattgc
     4741 ggacggttac ggcgaggatg cggcgaccgc tggagtcgaa aatcgatccc cggttgccgg
     4801 aaagcgaatc ggatgatgtc gtctggcggc gggccttcgc ggccaggaac tggctggccg
     4861 gcccctggta gcgccacgcc tggatcagaa ccgcgaccat ggcgagcccc agaagaccca
     4921 ggacgacggc catcctgaat acgggacggc gctcgattct gtcggttttg ccggtcaatc
     4981 cccgcatcac cgtctcgacg cccttctgca gcatgccgcc ctgacccatc ccgccacctg
     5041 cctactcggc ctgtccaccg gccgccctgg ccatgcaccg ctttcccgtc ggggaatcct
     5101 tcagcagcga tgcacacttc tcaaatgcct ccatcgcctc cgtcgtccgt cccgccttct
     5161 gatggatgac ccccaggtgg taataggact cgccgaagtc cggacagaac cttgtcacct
     5221 tctcgtaatt ttcctgggcg tcccgaacca tgtgcttctt aaggaacaca tgtcccaggt
     5281 tgaggtatcc aagacagaac tttggattaa ggaagaccgc catgtcgtat gcctttcggg
     5341 ccgattccag atcacccatt tccatatagg cgcggccgat attggcgtgg gccaggtaag
     5401 gtgtcgg
//
LOCUS       DAOPPA010000192         5403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16589_length_5403_cov_3.449140, whole genome shotgun sequence.
ACCESSION   DAOPPA010000192 DAOPPA010000000
VERSION     DAOPPA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16589_length_5403_cov_3.449140"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKH54_07005"
     CDS             <1..787
                     /locus_tag="PKH54_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC99675.1"
                     /translation="IGAAVRDDFIDVFRHPARLVVLASAGFGILALAALVGPMLMNEA
                     SGISMARHDDPGLTAHGGDQDGQCSHDQGRKVVETQRGVSPDGHPWIGSPAPKIEIHE
                     FTDYECPFCRKAHMMVRQLVASNPDVRVYHRHLPLDNRCNPSLEQPFHKRACELSKIS
                     ICAGEQGRFWEMNDFLFQHSQEIGGDNLNPNTIAERLELDMDRFQCCINDESRFAVMK
                     ADIEEGNRRELKGTPAFIINDSVYYGKIPSEALVPLPVVQPSE"
     gene            853..1842
                     /locus_tag="PKH54_07010"
     CDS             853..1842
                     /locus_tag="PKH54_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99676.1"
                     /translation="MNRAAFLIVPVAFLACVVLISAGCNQGNGVAGGGDMTADFFNEV
                     EALKMKMAACAPGQEIFDWGPGVNTLPILDGLSATMAEELYSTIRYANSAVSCEQFFL
                     SLNSELPPQTCAEPGRECRGSVAMVCLAGAERNYRIDIDCGDMDLGCLDGRCTIGVCE
                     SGECDGDTLVTCDGKSLKNEFRCGRLGLSCGYADNDLQCIGEGEQCSITSVAPACQGN
                     ILNFCLGGRLANIDCEKISGGRRECNQSWLDANQEITPLEIVASFLDRVCSQKYAECQ
                     DGASICSEDGEAMFCRDGLYEDVYCPYFGFENCTPPGPGIEKATCTGFVEILE"
     gene            1895..3124
                     /locus_tag="PKH54_07015"
     CDS             1895..3124
                     /locus_tag="PKH54_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC99677.1"
                     /translation="MNRPWFPRALTIVVLAAAATGCESESAGFFTVSGTVRDRDGGPL
                     EGISVTCDDMKGTAVSDVDGNFDLKCVLPERQLPEDPAPLAVVMFQGGGFAPSAKTVM
                     PVEDVRIGMEVIMEKRASSHDVSIPAGNVSVPVLVETMGLSFRKDALLDETGIPVAGT
                     ASFKAASWDISLPVEMDEGTNKIITDAINPPLNRQAVVPGDGTWLRPLAAGWLDTAPA
                     SINPNVGIDLQMFSQFADDALGRQISDPEDSRIFFLDTTTGLMKEIDDATLIAQNQVG
                     TKVIDPGLWVWMAPHAQHACFDVTVNKGDNPVNGAQVKAFEMFGDDREKSLDEAIGSE
                     DGHYCLRAVQGKSVQIEVVIPGKDGLLTLSRDLMSGGTAACGDGCNPIKFEFPCEINK
                     DCAPDAECLEGECVARD"
     gene            3439..4788
                     /gene="dnaA"
                     /locus_tag="PKH54_07020"
     CDS             3439..4788
                     /gene="dnaA"
                     /locus_tag="PKH54_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOC99678.1"
                     /translation="MQNLWDRARCLLKDRLDPDAFNKWIAIIRPCENEGGRLTLEVPD
                     TYTAKHIQDNFAEKIKDALWDTTHRSVGVDFRVAQPDPSASLPVSAVPSDRKAGGQES
                     PGIQNDIDRLKERFTFENFIVGDCNSFVHSAGLAVSRDPGGAYNPLFIYGGVGLGKTH
                     VLHAIGHQIRRENPRLKIVCTSGETFTNIVMNAVNSKSMDSLRSKFRGLCDVLLVDDI
                     QFVSSSQFVQTELFHTFNALYNDGKQVVITSDQPPSALDVLMDRLRSRFEWGLIADIQ
                     PPDEETRVRILLRKAQIEEYDLPFDVAEFLASRYSGSVRELEGALNRVSAHALLMKQA
                     ITLDMARRISIKTLNEGRGRVTSERVIRTVADYYNLSPVDIRGPKRHRAVSIPRYIAM
                     GIIRDHLKFSLPQIGRIFDNRSHTTVLSGLKTLDKMVQTEASVNNAIRDICLTLGFM"
     gene            4889..5158
                     /gene="rpsO"
                     /locus_tag="PKH54_07025"
     CDS             4889..5158
                     /gene="rpsO"
                     /locus_tag="PKH54_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOC99679.1"
                     /translation="MPLATVKKQEIIEKFKTHDSDTGSADVQIALLTERIRYLTEHFK
                     TNKQDHHSRRGLLKLVGRRRRLLDYIKASDKDRYQNLITELGIRK"
     gene            5277..>5403
                     /locus_tag="PKH54_07030"
     CDS             5277..>5403
                     /locus_tag="PKH54_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99680.1"
                     /translation="MFLRETIDFFGKPLTIETGRLAKQANGAALVTLGETVVLVTV"
BASE COUNT         1202 a         1558 c         1555 g         1088 t
ORIGIN      
        1 aatcggggca gccgtccggg atgacttcat cgacgtcttc cgccacccgg cccgccttgt
       61 agtgctcgca tcggccggat tcggcatact tgccctggcc gcgctcgtcg ggccgatgct
      121 gatgaacgaa gcctccggca taagtatggc gcgtcatgac gatcccggcc tgacggcgca
      181 tggcggggac caggacggcc agtgctcgca cgaccagggc aggaaggtcg tcgagacgca
      241 gcgtggggtt tcgccggacg gccatccctg gatcggttca ccggcaccga aaatcgaaat
      301 ccacgaattc acggattacg aatgcccgtt ctgcagaaag gcccacatga tggttcgaca
      361 actcgtggct tcaaacccgg atgtacgggt gtatcaccgc cacctgcccc tggacaacag
      421 gtgcaatcct tcccttgagc agcctttcca taaaagggcc tgcgaactct cgaagatttc
      481 catctgcgcg ggcgaacagg gacgcttctg ggaaatgaat gattttctgt tccagcactc
      541 gcaggaaatc ggtggtgaca acctgaaccc gaacacgatc gcggaacgcc tggaactcga
      601 catggatcgc ttccagtgct gcatcaacga cgaatcccga ttcgcggtga tgaaggccga
      661 catcgaggaa gggaacagaa gggagttgaa aggtactcca gctttcatta tcaatgattc
      721 cgtatattac ggtaaaatcc cttccgaagc tcttgtgccc ttgccggtgg tgcaaccgtc
      781 ggaatgacgg aatttcgggc acggcgcgac gccggacggc gtccggattg caaccttcaa
      841 aggtgatccc tgatgaatcg tgccgcattc ctgattgttc ccgtggcttt cctggcctgc
      901 gtagtcctca tctcggccgg ctgcaaccag ggcaacggtg ttgccggcgg cggcgacatg
      961 acggccgact tcttcaacga agtcgaagca ctgaagatga agatggccgc ctgcgcaccc
     1021 ggccaggaaa tttttgactg gggccccggc gtcaacacgc tgccaatcct tgacggactt
     1081 tccgcaacca tggccgagga gttgtactcg accatccgtt acgcgaattc ggccgtttcg
     1141 tgcgaacagt tctttctgtc gctgaactcc gaattgccgc cgcaaacctg tgctgaaccg
     1201 ggacgcgaat gtcggggatc cgtcgcgatg gtctgccttg cgggcgccga aaggaactat
     1261 cgaatcgaca tcgactgcgg ggacatggat ctgggctgcc ttgatggtcg ctgcaccatt
     1321 ggtgtctgcg aatcgggaga atgcgacggg gacaccctgg tgacgtgcga cggaaagagc
     1381 ctgaaaaacg aattcaggtg cggaaggctc ggcctgtcct gcggatacgc cgataacgac
     1441 ctgcaatgca ttggcgaagg ggaacagtgc tccatcacct ccgtggcgcc ggcgtgccag
     1501 ggaaacatcc tgaacttctg tctgggcggc aggcttgcaa acatcgactg cgagaagatc
     1561 tcgggtggtc gaagggaatg caaccagtcc tggcttgacg ccaaccagga gatcacgccg
     1621 cttgaaatcg tcgcttcttt cctggacagg gtgtgcagcc agaagtatgc cgaatgccag
     1681 gacggcgcgt cgatctgctc cgaggacggg gaggccatgt tctgccgcga cggcctctat
     1741 gaggacgtct attgtcctta cttcggcttt gagaactgca cgccacccgg gccgggaatc
     1801 gaaaaggcga cctgtaccgg gtttgtggag atacttgaat gaacgcgcat gggaaatccg
     1861 gttgcttccg gattcccggc aaaaggggga acacatgaat cggccgtggt ttccgcgtgc
     1921 cctgacgatc gtcgtgctgg ccgccgccgc aaccggctgt gaatccgagt ccgctggatt
     1981 cttcacagtc tcggggactg tacgcgatcg tgacggaggg cctctcgaag gcatctcagt
     2041 gacctgtgac gacatgaaag ggacggcggt ttcggatgtt gacggcaatt tcgaccttaa
     2101 atgcgtcctg cccgagaggc agcttcccga agaccccgcc ccccttgcgg tcgtgatgtt
     2161 ccagggcggc gggttcgctc cgtcggcaaa gacggtaatg ccggtagaag atgtacgaat
     2221 cggcatggaa gtcatcatgg aaaagcgtgc gtcgagccac gacgtctcga tccccgcggg
     2281 caacgtatcg gtgccggttc ttgtcgagac gatggggctt tcgttccgca aggacgcgct
     2341 tctggatgaa acgggcattc cggttgccgg caccgcttcg ttcaaggccg cctcgtggga
     2401 catcagcctt ccggtcgaga tggacgaggg aacaaacaag ataatcaccg acgcgatcaa
     2461 tccgccgttg aaccgccagg cggtggttcc aggcgacggc acatggctca ggccccttgc
     2521 ggcaggctgg cttgacactg caccggcctc gataaacccc aacgttggaa tcgacctcca
     2581 gatgttcagc cagttcgccg acgacgcact tgggcgccag atttcggatc cggaggactc
     2641 aaggatcttc ttcctcgaca cgacgaccgg cctgatgaag gaaatcgatg acgcgacgct
     2701 gatcgcacag aaccaggtgg gcacgaaggt gatcgacccc ggactctggg tctggatggc
     2761 cccgcatgcg cagcacgcct gcttcgatgt caccgtcaac aagggcgaca acccggtgaa
     2821 cggcgcccag gtcaaggctt tcgagatgtt cggggatgat cgcgagaagt cccttgacga
     2881 ggctatcggc tccgaagacg gccattactg cctgagggcc gtccagggaa aatcggttca
     2941 gatcgaggtc gtcatccctg gaaaggacgg gctgcttacc ctttcgcgtg acttgatgtc
     3001 cggcggaacc gcggcctgcg gcgacggctg caacccgatc aaattcgaat ttccatgtga
     3061 aatcaacaag gactgcgctc cggatgccga atgtctcgaa ggggaatgcg ttgcccgcga
     3121 ctgaccggaa acccccaccg aagcttccat tcccaatatt gttcaagaaa caccgaaggt
     3181 tgtggaatca ggatcgcaag gctctgtaat cattggctaa aaccaaagaa ttcttaaaat
     3241 gtcggaactt atggcgccgg gggtgttgcc gcaccctttc atcgccgcga taacccattg
     3301 aaatcattgc ttcaaaaaat tttttccatt caggggttgt atcgttttca acaacgaatt
     3361 agtttccact tcaccaagag ccttcggtgc tcgaatccaa actcggaaac aacaatctgg
     3421 ggtgaacggg cggagtttat gcagaatctt tgggacagag cgcgctgcct tttgaaagac
     3481 agacttgatc cggatgcttt caacaaatgg attgcaatca ttcgtccatg tgaaaacgaa
     3541 ggtggaaggc tgacccttga agtaccggac acgtacacgg ccaagcacat tcaggacaac
     3601 ttcgccgaaa aaattaagga cgccctgtgg gataccaccc acagaagcgt cggcgttgat
     3661 ttccgcgttg cccagcccga cccgtcggcc agcctccctg tttccgcggt tccgtccgac
     3721 agaaaggctg gcggccagga gtcgcccggc atccagaacg atatcgatcg cctgaaggaa
     3781 cgcttcacct tcgaaaactt catcgtcggc gattgcaact ctttcgtgca ctcggcgggc
     3841 cttgccgttt ccagggatcc gggcggggcc tacaacccgc tgttcattta tggcggcgtc
     3901 ggacttggca agactcacgt gctccatgca ataggacacc agatcaggcg cgagaatccg
     3961 agactgaaga tcgtctgcac cagtggcgag actttcacga acatcgtgat gaacgcggtc
     4021 aacagcaaga gcatggacag cctgcgatcg aagttccgcg gcctttgcga cgttctgctc
     4081 gttgacgaca ttcaattcgt ttcaagcagc cagttcgtgc agactgaact gttccacacg
     4141 ttcaatgccc tgtacaacga cggtaagcag gtcgtaatca ccagcgacca gccgccttcg
     4201 gccctcgacg tgctgatgga tcggctgcga agccgcttcg aatggggcct gatcgcggac
     4261 atccagcccc cggacgagga aacccgcgta aggatcctcc tgcgcaaggc ccagatcgag
     4321 gaatacgacc tgcccttcga cgtggccgaa ttccttgcct ccaggtattc gggatccgtg
     4381 cgcgagctgg aaggcgcgct gaaccgggtt tcggcccacg ccctgctgat gaaacaggcc
     4441 atcacgctgg acatggcccg ccgcatatcg atcaagactc ttaacgaagg tcgtggccgc
     4501 gttacttcgg aacgcgtcat caggaccgtg gccgactatt acaacctcag cccggtcgat
     4561 atccgtggcc ccaagcgaca tcgcgcggtg tcgataccgc gatatatcgc gatgggaatc
     4621 atcagggatc acctgaagtt ctcgctgccg cagatcggcc gcatttttga caaccgcagc
     4681 cacaccaccg tgctttccgg attgaagact ctcgacaaga tggttcagac cgaagcatcg
     4741 gtcaataacg ccatccggga catctgcctt accctgggtt tcatgtgatc gtttcagaca
     4801 ctattccggt ggggaaaaat acacttgaca ccgggataac cgacgcctat catactggcc
     4861 ggttttaatg gacgaagtgg aggaacggat gccacttgcg acagtcaaga agcaggaaat
     4921 aatcgaaaag ttcaagacgc acgactccga caccggctcg gctgacgtgc agatcgctct
     4981 gctgacggag cgtatccgtt atctgacgga gcatttcaag accaacaagc aggatcacca
     5041 ttcccgtcgt ggactgctga aactggttgg ccgccgccgg cgcctgctgg actacataaa
     5101 ggcgtccgat aaggatcggt accagaatct catcacggaa ctgggcatac gcaagtaagc
     5161 ctgatgacgc cgttcagatt ccgaccttgc ctcatcgtct gcaggctttt tcgaaatggc
     5221 attgcggaca tcatcgatcc ccttaatttt tggcaacaaa acagaggaat aatattatgt
     5281 ttttgcggga aaccatcgac ttcttcggca agccgctgac catcgagacc ggtcggctcg
     5341 ccaagcaggc aaacggcgcg gcgctcgtca ctctcggcga gacggtcgtg cttgtcaccg
     5401 tat
//
LOCUS       DAOPPA010000193         5386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16656_length_5386_cov_3.861002, whole genome shotgun sequence.
ACCESSION   DAOPPA010000193 DAOPPA010000000
VERSION     DAOPPA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16656_length_5386_cov_3.861002"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKH54_07035"
     CDS             complement(<1..687)
                     /locus_tag="PKH54_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99681.1"
                     /translation="MTATLVAALVLVVGAAGAASTAEVDKNGIRWVKVGMAPEVADKP
                     GAEAVPIGSGASGRLRFEMSATEITNGQYLLCVADGACKLHWDDETCHQRTTGDVFRG
                     RLPDSFRLSDQPVVCVSWHDAQAFAKWAGGRLPTEAEWEYAASGGGRADPYPWAVGDA
                     SCERAIMVSGQTGGCGTGATWPVCSRPPGNSPDGLCDMAGNVWEWVADRWAPAPGTPV
                     IQGMVDYSAYR"
     gene            complement(716..2578)
                     /locus_tag="PKH54_07040"
     CDS             complement(716..2578)
                     /locus_tag="PKH54_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC99682.1"
                     /translation="MRFRLSVVVMTALTVLASETIAQPAEPKPAVAVFDIVVEGLKIP
                     EGTVRRLTRYLGVRLMMGGRYQIIPEDQLRGALTALKLDSQKDCYDDKCRVQLGLEVA
                     ASSNLIVNLWKVERKCQMTAVLYDLRTFTGQEGAAVRDVDCGEAGLTKGIEQLAEQLG
                     GQAPDEEPGGRGQSPVVTAQQPLLPPPPVAQSSPVGGARDALPPPVKAQTGFLVVEGQ
                     PRGARVDITGPASFGSKGTASTSLPVRSIQVPVGEYRIQVSMDGHDTEERTVRVHWDA
                     TEIVKIDLVQSFGQIQISGRPEGAKGRLQCQKGFDREFGLPSVASPWTVTVPRGPCRM
                     VVEGDGYGTYDETFDVAGGSTVPRSVELVRFPVGTGDVAGSGIRWVRIPGGTFRMGSN
                     RGTRDEKPVHAVTVGTFEIAATEVTVAQYRKCVNAGVCTRPHWDDGACFNFDGTDWKK
                     GSLPASFRNDNQPVVCIDWNQAATYAEWVGGRLPTEAEWEYAARSAGRDQKYPWGDRA
                     PDCTRVVFADGSQGCGRISTWPVCSRPSGNTAQGLCDVAGNAWEWVSDWYDASYYEAS
                     PTGNPSGSDSGSRRVMRGGSWFCPADQMRAANRFNRVPNMASSDFGFRPVRTAR"
     gene            complement(2794..3681)
                     /locus_tag="PKH54_07045"
     CDS             complement(2794..3681)
                     /locus_tag="PKH54_07045"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOC99683.1"
                     /translation="MLVSAVMLAHAAAMSGSQDGIGPGLPVGRTGGDWARPGATGQNL
                     PVGGNGLDRDNGRRSPRPRRFAGPLLMTMGDIIQEPNLIFRIIVASLFSAYTVVRLAC
                     TWRLRHLKDPAAVRQPLHNRWRTKTRYEPGYLLALRDIVFGAFVATGVAYPFYPPFVA
                     AFDVALPDWLRYVAVAFAAGGLVLLASTHRALGEYWSAGLELKDSHRLISNGPYRYVR
                     HPMYTAVFLFFGAVAVTTANWLIIGPAMAIILVLSVRIRFEEAMMLDRFGETYSAYAA
                     DTGALVPRPRFWRRLRQGK"
     gene            3816..5321
                     /locus_tag="PKH54_07050"
     CDS             3816..5321
                     /locus_tag="PKH54_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99684.1"
                     /translation="MKLPKSKMRTPGIPKVVRHLALMLGVALAATPASADPVLDMVGA
                     AITPMPFSAKIVSTGTDAVYFNPTLMLDQKPGFRIGFFYMRHNLNIGYMDRPADADIS
                     LDAYSSRPIDETPLPSDRMRPTPTSALVNKRGAHNPIDNTYYMNISHSAHFIKDKLAF
                     GVYMLIPTNSFQSQRPFFADEREQIFSNSLHFELFGDRMRNYLFSMSIAGRPAKWVSL
                     GIGLTLSMVSHTTSRVLVTGIGTGSAPVISPEVSVRAVAVPYAAVTFAPIEGLKIATT
                     FHWKSQSPMKTSHQQQIWGYDKSGEPYMTENTYDFTYSSDPMRLALGASWTGKGTDRL
                     DYTVAATFTWARWSDYRDRQADKPSKAWSDNFPVTAGVLFSVDKAHEIGLDMTFVKSP
                     VPYQDGRTNYIDNDRFGIATGYGYKFLVKKKYLLSFGVSYQFQHLIPRTTLKSSSAAN
                     PVVDEFADSIFYGSDEPVASSDGLQTNNPGFPGFSSSGWLMAAGLYFRFQH"
BASE COUNT         1094 a         1762 c         1540 g          990 t
ORIGIN      
        1 gcggtacgcc gaataatcca ccattccctg gatgaccggg gttcccgggg cgggcgccca
       61 ccggtcggcc acccattccc agacgttgcc ggccatgtcg caaaggccgt ccggcgaatt
      121 gccaggcggc cgcgaacaga ccggccatgt ggcgccggtt ccacatccac cggtctggcc
      181 ggacaccatt atcgcccttt cgcacgaagc atcgcccaca gcccacggat acggatcggc
      241 gcgcccgccg ccggaagcgg catattccca ttcggcctcg gtcggcaggc ggccccccgc
      301 ccacttcgcg aaggcctgcg catcgtgcca ggaaacgcac accaccggct ggtcactcag
      361 acggaaactg tccggaagac gcccccgaaa cacatcgccc gtggtcctct ggtgacacgt
      421 ctcgtcgtcc caatgcagct tgcaggcacc atcggccacg caaagcaggt actgcccgtt
      481 cgttatttca gtcgccgaca tttcgaaccg aaggcgcccg gatgcaccgg aaccaatggg
      541 gacggcctcc gcgcccggct tgtccgcaac ttccggggcc atcccgacct tgacccacct
      601 tattccgttc ttgtccacct cggcggttga tgcggcaccg gccgccccga caaccagaac
      661 cagagcagcc accaatgttg cagtcatcga catgttcatg tgcattccgt gacaatcacc
      721 ttgccgttcg gacagggcgg aagccaaaat ccgaacttgc catgttcgga acccggttga
      781 agcggttggc ggccctcatc tggtcagccg gacaaaacca cgatccaccg cgcatgacgc
      841 gcctggatcc cgagtcgctt ccggacgggt tgcctgttgg acttgcttca tagtaagacg
      901 cgtcatacca gtccgacacc cattcccacg cgtttccggc cacgtcgcaa aggccctggg
      961 ccgtattgcc cgacggcctg gaacaaaccg gccacgtcga aatccgcccg cagccctggg
     1021 aaccgtcggc gaaaacaacc ctggtgcagt ccggagccct gtcgccccat gggtatttct
     1081 gatccctgcc ggcgcttctt gccgcgtatt cccattcggc ctcggtcggc aggcggcccc
     1141 cgacccattc cgcgtacgtc gcggcctggt tccagtcgat gcagacgaca ggctggttgt
     1201 cgttacggaa ggacgcggga agactgccct ttttccaatc cgtcccatca aaattgaagc
     1261 aggcgccgtc atcccagtgc ggcctggtgc acacaccggc attgacgcac ttcctgtact
     1321 gcgcgacggt gacctcggtc gccgcaatct cgaacgtccc cacggtcacg gcgtgaaccg
     1381 gtttttcatc gcgcgtgccc ctgtttgatc ccatcctgaa agtaccgccc gggattcgca
     1441 cccatcggat gccacttccg gccacgtccc cggttccgac gggaaagcga accagttcaa
     1501 ccgaccttgg aacggtcgaa ccgccggcca catcgaacgt ttcgtcgtac gtgccataac
     1561 cgtcgccctc gacgaccatg cggcacgggc cccttggaac ggttaccgtc cagggacttg
     1621 caaccgatgg aaggccgaat tcacgatcga atcccttctg gcactgaagg cggcctttcg
     1681 cgccctccgg cctgccggaa atctggatct ggccaaacga ctgaaccaga tcgatcttca
     1741 cgatttccgt ggcgtcccag tgaacccgaa ccgtcctttc ttcggtgtca tggccgtcca
     1801 tgcttacctg gattcgatac tcgcccacag gaacctgaat cgacctgacc ggtaacgagg
     1861 ttgacgcggt ccctttcgaa ccgaacgagg caggcccggt gatatcgacc cgcgcgcccc
     1921 tgggctggcc ctcgacgacc aggaatccgg tctgggcctt taccggtggc ggcaaggcgt
     1981 cccgggcgcc gccgacgggg gacgattggg ccacgggcgg tggaggcaac aagggctgct
     2041 gtgcagtgac caccggtgac tggccccgtc cgcccggctc ttcatcgggc gcctggccgc
     2101 caagctgttc ggcaagttgt tcgattccct tggtcaaacc ggcctcgccg caatcgacgt
     2161 cccttacggc ggccccttcc tgcccggtga aggttcgaag gtcataaagc accgcggtca
     2221 tctggcactt ccgttcgacc ttccacaggt tgacgatcag gttgctgctt gccgccacct
     2281 ccagcccaag ctgcacgcgg catttgtcgt cgtaacaatc cttctgggaa tccagcttca
     2341 gggccgtcag tgcacccctt aactgatcct cgggaatgat ctggtaacgt ccgcccatca
     2401 tcaggcggac accaaggtac ctggtaaggc gcctgacggt tccttccggg atcttcaagc
     2461 cttccaccac tatgtcgaat acggcaaccg cgggtttggg ctcggcgggc tgggcgatgg
     2521 tttccgaagc aaggacggtc aaggctgtca tgacaacgac cgaaagacgg aaacgcacgc
     2581 aatcaccccg aattcagaca aatggatcag ccgccgattt ataacatgac cggatgcttt
     2641 gtcacagcgg gaccggacgg cttaacacgt cgccatgaag aatggacacc cagggtggaa
     2701 gaatgaagag tcattcatgg tacaccgggc gaattttcat tcgcccgtac ggagccaggg
     2761 actgatagtt gaagggcgga gagggtccag gggtcatttg ccctggcgaa gccggcgcca
     2821 gaagcgcggg cgcgggacaa gggcgccggt atctgcggcg tacgcggaat atgtctcgcc
     2881 gaaccggtca agcatcatgg cttcctcgaa gcggatgcgc accgacagga caagaatgat
     2941 ggccatggcc ggaccgataa tcagccagtt ggcggtggtc acggcgactg ccccgaaaaa
     3001 caggaagacc gccgtgtaca tcggatgacg gacataacgg tacggcccgt tcgaaatcag
     3061 ccgatggctg tccttcaact ccaggccggc cgaccagtat tcgcccaggg ccctgtgcgt
     3121 tgaagcaagc agcaccagac cgcccgccgc gaacgccacc gcgacgtacc gcagccagtc
     3181 gggaagcgcc acgtcgaaag cggcgacgaa cggcggataa aacggatatg cgacaccggt
     3241 cgcgacgaat gccccgaaaa cgatatcgcg caaagccaga agataaccgg gctcgtacct
     3301 tgttttcgtg cgccaccggt tgtgcagcgg ctggcggacg gcggccggat ccttcaggtg
     3361 gcgaagcctc cacgtgcagg caagccgcac gacggtgtat gccgaaaaaa gcgacgcgac
     3421 gattatcctg aagatcaggt ttggttcctg gatgatgtcc cccatcgtca tcaataacgg
     3481 cccggcaaaa cgcctgggcc ttggcgaccg acggccgttg tcccggtcaa gtccattgcc
     3541 gccgaccggc aggttctgtc cggtcgcgcc cggtcgcgcc caatcaccac cggttctgcc
     3601 caccggaaga ccgggcccga taccatcctg gctaccggac atcgccgccg catgcgccag
     3661 cataaccgca cttacaagca ttgccaacaa cggccaccgc gatcaaatgc cgcataaccc
     3721 gccgtatggc aatcgaaaat cgactttttc cgtcgtgatt gtggcctttc gggttaactg
     3781 acataatctg ttcttccctg atccgggaat catctatgaa acttcccaaa tcgaaaatgc
     3841 ggacgccagg catcccgaaa gttgtcaggc accttgcatt gatgcttgga gtcgcactgg
     3901 ccgccacgcc ggcgtcggcc gacccggttc ttgacatggt cggcgccgcc atcacgccaa
     3961 tgcccttttc cgcaaagatc gtttcgaccg gcaccgacgc cgtctatttc aatccgaccc
     4021 tgatgcttga ccagaaaccg ggcttccgga tcgggttctt ttacatgcgc cacaatctga
     4081 acatcggata catggacagg ccggctgacg ccgacatctc gctcgacgcc tattcaagcc
     4141 gcccgatcga tgaaaccccg ctgccttccg accgcatgcg gccgacgccg acgtccgcgc
     4201 ttgtcaacaa acggggcgcg cacaacccga tcgacaacac gtattacatg aacatttccc
     4261 attcggcgca cttcatcaag gacaagctgg ccttcggcgt ctatatgctg attcccacca
     4321 acagcttcca gtcgcagcgg ccattcttcg cggacgaaag ggaacaaatc ttttcgaact
     4381 cgctgcactt cgagctcttc ggcgacagga tgcgcaatta cctgttttcg atgtcgattg
     4441 cgggacggcc ggccaagtgg gtgtcgcttg gaatcgggct gacgctttcg atggtttccc
     4501 acacgaccag ccgcgtcctg gtgaccggca tcgggacagg gtcggcgccg gtcatcagcc
     4561 ccgaagtcag cgtgcgcgcg gtcgccgtgc cgtatgcggc ggtcaccttc gcgccgatcg
     4621 aagggctgaa aatcgcgaca acgttccact ggaaatcgca aagcccgatg aagaccagcc
     4681 accagcagca gatctggggt tatgacaagt cgggcgaacc gtacatgacc gaaaatacat
     4741 acgatttcac gtactcaagc gacccgatgc gactggccct gggcgcttcc tggaccggca
     4801 agggaacgga caggctggat tacaccgtgg ccgcgacctt cacgtgggcc aggtggtcgg
     4861 actaccgcga caggcaggcc gacaagccgt caaaggcatg gtccgacaac ttccccgtga
     4921 ccgcgggcgt cctgttttcc gtcgataagg cgcatgaaat cgggctggac atgacctttg
     4981 tcaaaagccc ggttccatat caggacggac ggacgaatta catcgacaac gaccggttcg
     5041 gcatcgcgac cgggtacgga tacaagttcc tggtgaaaaa gaagtacctg ttgagcttcg
     5101 gcgtgtcgta ccagttccag cacctgatcc cgcgaacgac gctgaagtct tcttcggcgg
     5161 caaatccggt cgtcgatgag tttgccgatt ccatcttcta tggttcggac gaaccggttg
     5221 cctcgtctga cggcctgcag acgaacaacc cggggttccc gggattttcc agcagcggat
     5281 ggctgatggc ggcgggcctt tacttcaggt tccagcacta gggcaggtgg cagtgaagat
     5341 gcgaaacagc ctggcgattc cggtgctcgg tgcggccatg gtggca
//
LOCUS       DAOPPA010000194         5385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16662_length_5385_cov_3.707129, whole genome shotgun sequence.
ACCESSION   DAOPPA010000194 DAOPPA010000000
VERSION     DAOPPA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_5385_cov_3.707129"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKH54_07055"
     CDS             complement(<1..218)
                     /locus_tag="PKH54_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99685.1"
                     /translation="MNRLTTLIPAAAMLFVAACSGDQAPIFASGVFEATEVIVSSEAT
                     GRLLEFTPVEGLEIAAGDVIGKVDCRQI"
     gene            complement(215..844)
                     /locus_tag="PKH54_07060"
     CDS             complement(215..844)
                     /locus_tag="PKH54_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC99686.1"
                     /translation="MRTKQQPDQNREQAILAAAEELFLDRGFALTSTVEIAKKAGCNQ
                     ALVHYYFRTKENLFQAIFARKIKMFMTALTSMDQQDLPFEKALEFIIEAHFDVLASNP
                     RMPFLILNELTTNPKRIEALKELIASEIDLRPIKHMQALLEQEIRKGTVRQTTMMDIA
                     MTMAGLNVMTFLARPIIGGVLDIGDDDYQELIAHRRKENVATILRSIRP"
     gene            complement(956..2593)
                     /locus_tag="PKH54_07065"
     CDS             complement(956..2593)
                     /locus_tag="PKH54_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99687.1"
                     /translation="MSEIPGVIVACLCLSMLVSCGKVATGDIIEPCDVREVFDATDTS
                     EAIETPETFDTAEAFDTTEAGDANDTNHAGDTTVALDTEDTEVVPAPEWPTCPQIESQ
                     VALSDKAAYFDEVAASRHLAGDGLLRNLYLKENLQDVELWYHVENVILWSGMYLASQA
                     FRYEVTGEQEALHNARTVVNALHHLTLVTGVSGLYGRSMYNPSVKYNTVAPENPGWTD
                     STAPGYEGWRFRNDVSKDGYAGLMFGYAAALEHFKDQELLTDIRARVREIIDHIMLNN
                     LQIVDTTGLVTEHGRLYQTAMDDFPGFNAMLTSSWIKIAQTELADTTLEDFYYGCLMR
                     VRDGADCGMLAQISELEAGYMSYMESMEQFLNLFLPGCGQNYDNFDMCYQAIYPLMRR
                     EQDPALRERLLWVVRNNMFYTDNPKHQSIDVIGNSMFTFIYAAVTGDGPEDPMLYNAV
                     DLAVCKMKEFPAEKFEREIARGQQEEVCQSRLDNPVAAETIPLSEYPFDNYLWRLDFF
                     EIPQHDIAENRRMVYSPEDYLVAYWLGRMNGLIPADL"
     gene            complement(2586..4343)
                     /locus_tag="PKH54_07070"
     CDS             complement(2586..4343)
                     /locus_tag="PKH54_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC99688.1"
                     /translation="MGSFGILVALVGGLAAALVFGYLAIKLKFSPIVGYLLAGILIGP
                     FTPGLHVDHHITEQFAEIGVLLMLFGIGLKFHLEELIAVWRIAVPGALIQSSLSTLAM
                     ATIMHFLGMSWIQGAILGVGICVASTVVMANVMAESHDLHSKIGHIAIGWTVVEDILT
                     VMLLLLLPILFGPNAAGTDKALEAIGMAGLKMFGLVAVVVVLGKWVVPWILNRIANTR
                     SRELFTLAVLVLAIGVAVGAAKLFGVSMELGAFLAGLAVGRSEFATRASGDAVPMRDA
                     FSILFFVSVGMLFDPESLFKAPVTILVVLAVVLLIKPAIAVFTVRLHRKPWALAIPVG
                     VAFAQVGEFSFILGSVALGLKLIDSNSFDAIVAASIISITINPSLYRYMRKFSQRLGR
                     RSEDMPAVQGDMVDPDKCVLVGYGPVGQIVCRLLRERGADITVVDLNLETIRRLKAAG
                     IKAIYGDVLRAGTLEDAGIATSGSLVLSADIEDGVEIVRKVRKMNPGIRILARCTHLR
                     DAEALRLAGADVVAAGEAEVGVALAEEVTAGEDWDDMTAAEHREIIRRRLYDNPNGRF
                     PTAVLNAENVNLEPGKTDV"
     gene            4471..>5385
                     /locus_tag="PKH54_07075"
     CDS             4471..>5385
                     /locus_tag="PKH54_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99689.1"
                     /translation="MNFRGEFFGFAFVAVCCSVIGCGPGDAGHDVVPGDLDGYVEDRI
                     HDEGVPDEGGDSGQRDQGSADYVDFDADSVGDQGGDVSADDLPDGQGDDSGPDAAIDG
                     DLADDSNAGDAVIDIPADGDAVQGDDVPESCDPILFPMAMIKETETAACQFTNHRTVI
                     KEGLLMDLWDVSFVSWESIDCNLVPITIRGFASKPAGSSGKIPGLVQMHGLGGCADPD
                     NATGPAALLGYFVIAPTGPGGAPSTNSPDCAASGGLPSGHDSGRRMFDTYPDPRGSWF
                     WGHSVAAMRAVTCLTTSADVDSTRLGMTG"
BASE COUNT         1066 a         1539 c         1603 g         1177 t
ORIGIN      
        1 tcgatctggc ggcagtccac cttcccgatg acgtcgcccg ccgcgatttc aagtccttcg
       61 accggcgtga attccagcag gcgtccagtc gcctcggacg acacgatgac ttcggtcgcc
      121 tcgaacaccc cggacgcgaa gatcggcgcc tggtcgccgg aacacgccgc gacgaacagc
      181 atggcagccg caggtatcaa tgtggtcagt ctgttcatgg cctgatgctc cttaagatgg
      241 tggccacatt ttcctttctg cggtgggcga tcagttcctg gtagtcgtcg tcgccgatgt
      301 ccagtacgcc gccgatgatc ggtcttgcca ggaacgtcat gacgttcagc cccgccatcg
      361 tcatggcgat gtccatcatc gtggtctgcc ggacggttcc tttcctgatt tcctgttcaa
      421 gcagcgcctg catatgcttg atcgggcgca ggtcgatctc gctggcgata agctccttaa
      481 gcgcctcgat ccgcttcggg ttggtcgtaa gttcgttcag gatcaggaag ggcatgcgcg
      541 ggttggacgc cagcacgtcg aagtgcgctt cgatgatgaa ttcgagcgcc ttttcgaatg
      601 gcagatcctg ctgatccatg ctggtcagtg ccgtcatgaa cattttgatc ttgcgggcga
      661 atatggcctg gaacaggttt tccttggtgc ggaagtagta atgcaccagg gcctggttgc
      721 agccggcctt tttcgcaatc tcgaccgtcg aagtcagggc gaagccgcga tccaggaaga
      781 gttcctcggc cgccgccagt atcgcctgtt ccctgttctg atccggttgt tgtttcgttc
      841 tcatggctaa taaggttatt taataaggct attaaatgtc aagaggaagg ttgtgaaggg
      901 cccaggggga agggggcagg ggagttgatg gcccgcccat acaagtcgca gtggatcaga
      961 ggtcggccgg aatcaggcca ttcatgcgtc ccagccagta tgcgaccagg tagtcttccg
     1021 gtgaataaac catgcggcgg ttttcagcga tgtcgtgctg gggaatttcg aaaaagtcca
     1081 gtcgccaaag atagttgtcg aacggatact cggaaagcgg gattgtttcg gccgcgacag
     1141 gattgtccag gcgactctgg catacttctt cctgctgtcc ccgggcaatc tcacgttcaa
     1201 acttttcggc agggaattcc ttcatcttgc agacggccag atccacggcg ttgtaaagca
     1261 tcggatcttc cgggccgtcc ccggtcaccg ctgcgtaaat aaaggtaaac atactgtttc
     1321 cgattacatc gatgctctgg tgcttcgggt tgtcggtgta aaacatgttg ttgcggacca
     1381 cccacagaag gcgttcacgg agggcgggat cctgttcccg ccgcatcagt ggatatatgg
     1441 cctgatagca catgtcgaag ttgtcgtagt tctgtccgca tcccggcagg aacaggttca
     1501 ggaactgttc catgctttcc atgtacgaca tgtacccggc ttccagttca ctgatttgtg
     1561 caagcatccc gcagtcggca ccgtcacgaa cccgcatcag gcatccgtag tagaagtctt
     1621 ccagtgtcgt gtcggcaagc tcggtctgcg cgattttgat ccagcttgac gtcagcatcg
     1681 cgttgaagcc gggaaaatcg tccatcgccg tctggtacag gcgcccgtgc tcggtaacca
     1741 gtccggtggt gtccactatc tggagattgt tcagcattat gtggtcgatg atttcgcgca
     1801 ccctggcccg gatgtcggtc agaagttcct ggtccttgaa gtgttccagc gccgctgcgt
     1861 atccgaacat caggccggca tagccgtcct tgctgacgtc gttcctgaaa cgccagcctt
     1921 cgtatccggg ggctgtcgaa tcggtccaac cgggattttc cggggcgacc gtgttgtatt
     1981 tgacggaagg gttgtacatg gaacgcccgt aaaggccgga tactccggtg accagtgtca
     2041 ggtggtgcag tgcgttcacg acggtcctgg cattgtgcaa ggcttcctgc tcgccggtca
     2101 cttcatatct gaatgcctgc gacgcaaggt acatcccgct ccacaggatg acattttcaa
     2161 cgtgatacca gagttcgaca tcctgcaggt tttctttcaa atacaggttt cgcagcagtc
     2221 catctccggc cagatgccga cttgcggcga cttcgtcgaa atatgccgcc ttatccgaga
     2281 gtgcaacctg cgactcgatt tgcgggcagg ttggccattc aggagcaggg acaacttcgg
     2341 tgtcctcggt gtccagggcg actgtggtat cgccggcatg gtttgtgtcg ttggcgtcgc
     2401 ccgcttcggt agtgtcgaat gcctccgccg tgtcaaacgt ctcaggcgtc tcaatcgctt
     2461 cgctggtgtc ggtagcgtcg aaaacttcac ggacatcgca gggttcgatg atatcccctg
     2521 tggcgacttt tccgcatgaa acaagcatcg acaggcagag gcacgcaaca ataacacccg
     2581 gtatttcaga catctgtctt tccaggctcg aggttgacat tttcggcgtt cagaacggcg
     2641 gtcgggaagc ggccgttcgg gttgtcgtac aggcggcggc ggatgatttc gcgatgttcc
     2701 gcggcggtca tatcgtccca gtcttccccg gcggttactt cctcggcaag ggcgaccccg
     2761 acctcggcct cgcccgccgc gaccacgtcc gcgccggcca gacgcagtgc ctcggcgtcg
     2821 cgaagatgag tgcacctcgc caggatccgt atgccgggat tcattttgcg gacctttcgg
     2881 acgatttcga ccccgtcctc gatatcggcg cttaacacaa ggcttcctga agtcgcgatt
     2941 ccagcgtctt caagtgttcc cgcgcgcagt acatctccgt aaattgcctt gattcccgct
     3001 gccttcaggc ggcggatcgt ttcaaggttc agatccacca cagtgatatc ggccccgcgt
     3061 tcacgcagca gacggcagac gatctgcccc accggtccgt atcccacaag gacgcatttg
     3121 tcgggatcaa ccatgtcgcc ctgaacggcc ggcatgtctt ccgaccttct tcccaggcgt
     3181 tgggaaaatt ttcgcatgta tcgataaagc gacgggttaa tcgttatgga aatgatcgat
     3241 gcggcgacaa ttgcgtcgaa tgaattgctg tcaatcagtt tcaggccaag cgcgacggat
     3301 ccaaggatga agctgaattc gccgacctgc gcgaatgcca cgccgacggg aatggcaagt
     3361 gcccatggct tccggtgcag ccgaaccgtg aaaaccgcga tggccggctt gatcagcagg
     3421 accaccgcca ggacgaccag tatggtgacg ggtgccttga aaaggctttc cgggtcaaac
     3481 agcatgccga ccgacacgaa gaaaaggatt gaaaacgcat cccgcatcgg caccgcgtcg
     3541 cccgacgcac gggtggcgaa ttcggaccgc ccgaccgcga gaccggccag gaacgcgccc
     3601 agttccatcg aaacaccgaa cagctttgcg gcaccgacgg caaccccgat ggcaagaacc
     3661 agcacggcca gcgtgaacag ttcccgggaa cgggtgttcg cgattctgtt aagaatccac
     3721 ggcacgaccc atttcccaag caccacgacg accgcgacca gcccgaacat ctttagcccg
     3781 gccatgccga tcgcttcaag tgccttgtcg gttccggccg cgttcgggcc gaaaaggatc
     3841 ggaagcaaca gcagcagcat gaccgtaaga atgtcctcga cgacggtcca gccgatggcg
     3901 atgtgtccga tttttgaatg aagatcatgg ctttccgcca tcacgttcgc catgacgacg
     3961 gtactggcga cgcagatgcc aacacccagg attgcgccct gaatccacga catccccaga
     4021 aaatgcatga tcgttgccat ggccagggtc gaaagtgaac tctggatcag tgcgccgggg
     4081 acggcgattc gccagacggc gatcagttct tccaggtgaa atttcaggcc gattccgaaa
     4141 agcatcagca gaacgccgat ctcggcgaac tgttcggtaa tgtgatgatc aacgtgaagg
     4201 ccgggtgtga acgggccgat aaggatcccg gccagaaggt agccgacgat cggtgaaaac
     4261 ttcagcttta ttgccaggta tccgaataca agggctgccg cgagcccgcc gaccagcgcg
     4321 accagtatcc cgaagctgcc caactgccca cctctatcga tacgccggga cggatgaccc
     4381 gaccgtcaag gtttatcgcg aaactcccga tatgccaagt atggatgctc tggtatatta
     4441 atgtgaagcc gtaacctgcc gggggcatga atgaatttca ggggcgaatt ttttggtttc
     4501 gcgttcgtgg cggtttgttg ctcggtcatc gggtgtggtc ctggtgatgc cgggcatgat
     4561 gtcgtgccag gcgacctcga cggctatgtg gaagaccgga ttcatgacga gggagtgccc
     4621 gacgagggtg gcgattccgg ccagcgggat caaggctcgg ctgactacgt cgatttcgat
     4681 gctgattcgg tcggcgacca gggtggcgac gtttcggccg atgacctgcc ggacggccag
     4741 ggggatgatt cgggcccgga tgccgccatc gatggcgatc tggccgacga ttccaatgct
     4801 ggcgacgcgg taatcgacat tccggccgac ggggatgcgg tgcagggtga tgacgtgccg
     4861 gagtcgtgtg atccaatcct gtttccgatg gcgatgatca aggaaaccga aaccgccgcc
     4921 tgccagttca ccaatcatcg tacggtcata aaggaaggtc tcctgatgga tctgtgggac
     4981 gtttcgttcg tttcctggga atcgattgac tgcaacctgg ttccgataac aattcgcggc
     5041 tttgcctcga agccggccgg ttcttccggg aagatccccg ggcttgtcca gatgcacggg
     5101 cttggcgggt gcgccgatcc ggacaacgcg accggtccgg cagctttgct ggggtacttc
     5161 gtgatcgcac cgaccggccc gggcggtgca ccgtcaacga attcgcccga ctgcgcggca
     5221 tccggcgggc ttccttccgg ccacgacagc gggcgccgga tgttcgatac gtatccggat
     5281 ccgcgtggaa gctggttctg ggggcattcg gtcgcggcca tgcgggccgt gacatgcttg
     5341 acgacaagcg ccgatgtcga ttcgacgcgt cttgggatga ccggg
//
LOCUS       DAOPPA010000195         5371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16724_length_5371_cov_4.605907, whole genome shotgun sequence.
ACCESSION   DAOPPA010000195 DAOPPA010000000
VERSION     DAOPPA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16724_length_5371_cov_4.605907"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKH54_07080"
     CDS             <1..495
                     /locus_tag="PKH54_07080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289593.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOC99690.1"
                     /translation="PADDLTDPAPATTFSHLDATTVLSRQIAELGIYPAVDPLDSTSR
                     ILDPAVVGMEHYSVARRIQECIQEYRDLQDIIAILGMDELSETQRQTVQRARKIQQFL
                     SQPFFVAEQFTGKEGRFVKVQDTVNGFKEILDGKHDDLPEQAFWMVGTIDEAVEKARK
                     MEAD"
     gene            512..931
                     /gene="atpC"
                     /locus_tag="PKH54_07085"
     CDS             512..931
                     /gene="atpC"
                     /locus_tag="PKH54_07085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOC99691.1"
                     /translation="MSLKVDVVEYDGLRISGLEVTEIALPGADGQLGILPAHIQMIST
                     VGIGPMRLTTPDGGVMLFSLAGGFLEILGDNVRILTESCEAAADIDVERAEKALKRAL
                     AKLETITPITHPEEHRVTTNALKRAQTRLAVASGKIV"
     gene            1161..1973
                     /locus_tag="PKH54_07090"
     CDS             1161..1973
                     /locus_tag="PKH54_07090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC99692.1"
                     /translation="MMKYTGYRIAAIFAHPDDEAYSCAGLLAMAAEGGAVVTVVCATR
                     GEAGRDREGIVPPGPALARLRTVEMGLSCRALGVQPPVFLDLPDGALQGHPELEPRIR
                     DVFSRLRPNVVITLGMDGAYGHRDHIAVTEAVIAQVMSMREGSTRRARRAPVRPRLLL
                     VQFPMGLFAKVRSFMERNLPHAIDTSDLPAADRMGIDPEDADARLDVRPWRGHKLRAL
                     AAHRSQLRDGDPESFLRDGLATALLDFELYTCASGPQLPAASDNPLDGIETE"
     gene            1970..3106
                     /locus_tag="PKH54_07095"
     CDS             1970..3106
                     /locus_tag="PKH54_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC99693.1"
                     /translation="MNILVITRDYPPHTNGGISTAVWYEVQGLVAAGHRCTVVSFDAF
                     RPVTQALLTDQVPRIDFSCSPAMITLSAPSAIPAALEAARQARPDVIHLHHSTLLDFA
                     LDLSGGHVPIVKTVHVIQHQMNWYRGLESTITSKAQARAVATCWRAVVMSRAAAQMMA
                     AEFPEFSDKFRVIAPGVPDQPGAADASWPRPDRIAVHAGRFSDIKGTEVLFDVARGLT
                     AADPRNHVVIAGGLPDGQPSERRYMRKWARGATGPDDERVSFPGWLDRSQLSALFLQS
                     SVYLATSVFETFGLSIAEAMLHGVPVVATACGGPQEIIEHGVSGMVVPVGDTRAIVAA
                     ASSILDSPDRCAEMGRAARQSILKRFDVRTHTESLEAVYREVIS"
     gene            3142..4848
                     /locus_tag="PKH54_07100"
     CDS             3142..4848
                     /locus_tag="PKH54_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99694.1"
                     /translation="MKRFLVLLMLVFVACGSGGGNQADSGADVADDAVSDVSGDAVND
                     TGSNVDARVDAATDAGIDTVVKPSCFEDITDNLPFQLFPDEGRFVLQVASAFDGEAVW
                     LAWSAMDERGTQDPDDDVSTIRAARIGCDRKILVEPFSVFPTGEGNCWEPDIVVSGDT
                     VMIVWHEDYQGYYARMGVLPIAPMPPEPVRAAAPLESISLEEADPDIVEPDDDAGDPD
                     ADTSDAVDDVADIIQDAASDQGSADAATDSGPVDAGTDVPYYPNDNMRIYFRTFKTDG
                     TPVMATQYRWMPPVDGVAAPVNNWIPRAAALPDGRFVIASTWANPNASVFQVVAQRFN
                     ADGSPEGDLIYPWPRLDEDQQNADVAVDSDGNIRMTWGHSIKEGEAWNTRIASMTIPA
                     GSSQPIEDSPVFFDVAGDLPNVAITPQDTVYTAFIDSSDPVNVVLVAETAGRASSGGK
                     MFGNDGDEKNWIHFNPVLNCNESGNVVALFREPPSTLQDKHTDLMLYRLAYNGTGDIA
                     AIGAGIEVNAPDQERTHDAQGYYKPSVTHVGAGHYLVTWAEPEKIGEVWHYKTFARFL
                     KP"
     gene            complement(4855..>5371)
                     /locus_tag="PKH54_07105"
     CDS             complement(4855..>5371)
                     /locus_tag="PKH54_07105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC99695.1"
                     /translation="IYGDNVEHKLGRLGFLVAYLVTGVAATLSYAALAGNSMTPLVGA
                     SGAISGILGLYFILFPKNRVKIFVFLCPLLVTVWHVPARIVLALYLLADNLLPLVLGA
                     DSGVAYGAHIGGFVAGVVLAVSGRAMSRAAGWLKKAFGPRQVVKKVPRSNRPDPRTVI
                     IDAEYVDDVRN"
BASE COUNT         1079 a         1664 c         1629 g          999 t
ORIGIN      
        1 ccggccgacg acctgaccga cccggcaccg gcgaccacgt tcagccacct tgacgcaacc
       61 acggttcttt cgcgccagat cgccgaactg ggcatttacc ccgcggttga cccgctggac
      121 tcgacctcgc gcatcctcga tccggcagtc gtgggcatgg agcattacag cgtcgcgcgg
      181 cgcatccagg aatgcatcca ggaatacagg gatttgcagg acatcatcgc gatcctcggc
      241 atggacgaac tgtcggaaac ccagcgccag acagtccaga gggcccgcaa gatccagcag
      301 ttcctgtcgc agccgttctt cgtcgctgaa cagttcaccg gcaaggaagg acggtttgtg
      361 aaggttcagg acaccgtcaa tggattcaag gaaatcctgg acggcaagca cgacgatctg
      421 cccgagcagg cattctggat ggtcggcact atcgacgagg ctgtcgagaa ggcccgcaag
      481 atggaagccg actgattgga acggggtgag catgtcgctg aaggttgatg ttgttgaata
      541 cgacgggctc cggatttccg gccttgaagt gaccgaaatc gcccttccgg gcgcggacgg
      601 tcagcttgga atcctgccgg cccacatcca gatgatttcg accgtcggaa tcggccccat
      661 gaggctgacc accccggatg gcggggtcat gctgttttca cttgccgggg gcttcctgga
      721 aatccttggc gacaacgtca gaattcttac cgagtcctgc gaggcggctg ccgacatcga
      781 tgttgaacgc gccgaaaagg ccctgaagcg ggctcttgcc aaactggaaa caatcacgcc
      841 gatcacccat cccgaggaac accgggtcac caccaatgca ctgaagcgcg cccagacgcg
      901 acttgcggtc gcgtccggca agattgtctg acacattgcc ttcaatcccg cggtcggatt
      961 ttcatccagt cgggcgaatt ttcatccagt cgggcgaatt ttcattcgcc catacggcgg
     1021 atggatttcc ggttccaccc ttgtacgtgc ggattacatc cgcccgccgc gatcatgcac
     1081 gctcccttcc ctgtacgggc ggattacatc cgcccgccgc ggtcatgcta gaatcgcagc
     1141 cgcacgcttc tttcagaacg atgatgaagt acaccggtta ccgcatcgca gccatcttcg
     1201 cccaccccga cgacgaggct tactcctgcg ctggccttct ggccatggca gcggaaggcg
     1261 gggccgttgt cacggtcgta tgcgcgaccc ggggcgaagc cggtcgggac cgcgaaggca
     1321 tcgtgccgcc tggccctgcc cttgcaaggc tcagaaccgt cgaaatggga ctgtcctgcc
     1381 gggctcttgg cgttcagccc ccggttttcc tcgatctgcc tgacggcgcc cttcagggac
     1441 atcccgaact tgaacccagg atccgggacg tcttttccag gttgcgcccg aacgtggtca
     1501 tcacgcttgg catggacggg gcctacggcc accgggatca catcgcggtc actgaagcgg
     1561 taatcgccca ggtcatgtcc atgagggaag gcagcacaag gcgcgcgcgc agagccccgg
     1621 ttcgcccaag gctgctgctg gtgcagttcc cgatgggcct tttcgcgaag gtccgttctt
     1681 tcatggaacg caaccttccc catgcaatcg acacatcgga tctacccgcc gcggaccgga
     1741 tgggaatcga tccggaagat gccgatgcca ggctggatgt caggccgtgg cgtggacaca
     1801 agctcagggc ccttgccgcc catcgttcgc aactgcggga cggggatccg gaatccttcc
     1861 ttcgggacgg gctggcgaca gccctgcttg atttcgagct gtacacctgc gcatccggac
     1921 cgcaactgcc ggccgcatcg gacaatcccc ttgacgggat tgaaaccgaa tgaatatcct
     1981 cgtaattaca agggactatc cgccgcacac caatggcggc atatcaacag cggtctggta
     2041 tgaagtccag gggcttgtcg cggcggggca tcgctgcacc gtggtgtcat tcgacgcgtt
     2101 caggccggtg acgcaggccc tgctgaccga tcaggtgccc cgcatcgatt tttcctgttc
     2161 gccggcaatg atcacgctgt ccgccccgtc ggccatccct gccgcgcttg aggcggcacg
     2221 gcaggcacgg ccggacgtga tccacctgca tcattcgacc cttcttgatt tcgcgctgga
     2281 cctgtcgggc gggcatgtac cgatcgtcaa gacggtgcac gtgatccagc accagatgaa
     2341 ctggtacagg ggccttgaaa gcacaatcac gtcaaaggca caggcacgag ccgtcgccac
     2401 atgctggcgg gccgtcgtaa tgtccagggc agccgcccag atgatggctg cggaatttcc
     2461 ggaattctcc gataaattcc gggtcatcgc tcccggcgtt cccgaccagc cgggcgcggc
     2521 cgatgcatcg tggccgcgac cggatcgaat cgcggttcat gcggggcgtt tcagcgacat
     2581 taagggtacc gaagttctgt tcgatgtcgc ccggggcctg acggcggcgg atccacgaaa
     2641 ccacgtcgtg atcgcgggcg gacttccgga cggccagccg tccgagcggc gatacatgcg
     2701 caagtgggcc cgcggcgcga cgggcccgga tgatgaacgc gtgtcatttc cgggctggct
     2761 ggacaggtcg cagctttcag cactgtttct gcaaagttcg gtttatctgg cgacaagcgt
     2821 gttcgagact ttcggactgt cgattgccga ggccatgctt cacggcgtgc cggttgtcgc
     2881 aaccgcctgc ggcggcccgc aggaaatcat tgagcacggc gtatccggca tggtcgttcc
     2941 ggttggcgac acccgggcca tcgtcgccgc ggcctcttcc atcctggatt ccccggatcg
     3001 ctgcgcggaa atgggccgtg cggccaggca gtcgattctg aaacgattcg acgtgcgaac
     3061 gcacaccgaa tcccttgaag ccgtgtatcg ggaagtcatt tcctgaccta taatccgcct
     3121 tgcctttcgc tggagtccgg aatgaagcgt ttcctcgttt tattgatgct ggtgttcgtc
     3181 gcgtgcggtt caggcggcgg gaatcaagcc gattcgggtg cggatgtcgc ggacgacgcc
     3241 gtatccgacg tttccggcga tgccgtgaac gacacgggaa gcaacgttga tgcacgcgtg
     3301 gatgcggcca cggacgccgg aatcgacacc gtggtcaagc cgtcgtgctt cgaagacatc
     3361 acggataacc tgcccttcca gctctttcca gacgaaggac gattcgtgct gcaggtcgca
     3421 tcggcctttg acggcgaggc cgtctggctg gcgtggagcg cgatggacga aaggggcacg
     3481 caggatccgg acgacgacgt ttccacgatc agggcggcaa gaattggttg cgacagaaag
     3541 atccttgtcg aaccgttttc ggtttttcca acaggtgaag gcaactgctg ggaacctgac
     3601 atagtcgtgt ccggcgacac cgtgatgatc gtgtggcacg aagattacca gggttactac
     3661 gctcgcatgg gtgtcctgcc gatcgcaccg atgccgcccg aaccggtccg cgccgcggcg
     3721 ccccttgagt cgatatcgct cgaggaagcg gatccggaca tcgtcgaacc ggacgacgat
     3781 gccggcgatc cggatgcaga tacatcggat gcagttgacg atgtggccga cattatccag
     3841 gatgccgcca gcgaccaggg ttcggccgat gccgcgaccg attcgggacc ggttgacgcc
     3901 ggcaccgacg taccgtatta cccgaacgac aacatgcgga tttatttcag gactttcaag
     3961 accgacggaa cgccggtgat ggcaacccag tacaggtgga tgcccccggt tgacggcgtc
     4021 gccgcgcctg ttaacaactg gatcccgcgc gcggccgcgc ttcctgacgg ccgcttcgtc
     4081 atcgcatcga cgtgggcaaa tccgaacgca agcgtgttcc aggtcgtggc ccagcgcttc
     4141 aacgccgacg gttcaccgga aggcgacctt atctatccgt ggcccaggct ggatgaagat
     4201 cagcagaatg ccgacgtcgc ggtcgattcc gacggcaaca ttcgaatgac gtggggacat
     4261 tcgatcaagg aaggcgaagc ctggaacacc aggatcgcgt ccatgacgat tccggccggt
     4321 tcaagccagc caatcgaaga ctcgcccgtg ttcttcgacg tcgccggcga tcttccaaac
     4381 gtcgccatca cgcctcagga cacggtctat accgcgttca tcgactcaag cgatccggtc
     4441 aacgtggttc tggtcgcgga aaccgccgga cgggcatctt ccggcggcaa gatgtttgga
     4501 aacgacgggg atgaaaagaa ctggatccat ttcaacccgg tacttaactg caatgaatcc
     4561 ggtaacgttg tcgcgctgtt ccgcgaaccg ccgtcaaccc tccaggacaa gcacacggac
     4621 ctgatgcttt accggctggc gtacaatgga acgggtgata tcgccgcaat cggcgcgggg
     4681 atcgaggtga acgcgccgga tcaggaaaga acccacgatg cccagggata ttacaagccg
     4741 tccgtgacac acgtcggcgc gggacattac cttgtcacct gggccgagcc ggaaaagatc
     4801 ggcgaagtct ggcactacaa gacgttcgcg cggttcctga aaccttgacc tgcatcagtt
     4861 tcgcacgtca tcgacgtatt ccgcatcgat tatgacggtt cttggatccg gccggttcga
     4921 cctcggaacc ttcttcacaa cctggcgcgg cccgaaggcc ttcttcagcc agcctgcggc
     4981 gcgagacatg gcgcgacccg aaacggcaag gacgaccccc gcgacaaaac cgccgatatg
     5041 ggcgccgtat gcaacgccgc tgtcggcacc cagaaccagt ggcagcaggt tgtccgccag
     5101 caggtaaagc gcaagcacga tgcgcgccgg aacatgccag accgtaacca gcagcgggca
     5161 gagaaagaca aaaatcttca cgcggttctt cgggaaaagg atgaagtaca acccgagaat
     5221 gccggaaatc gccccggaag cccccacaag cggggtcatc gaattcccgg ccagcgccgc
     5281 gtacgaaagc gtggcggcaa caccggtcac cagataggcg accagaaagc cgagccggcc
     5341 cagtttgtgt tcgacgttgt cgccgtatat c
//
LOCUS       DAOPPA010000196         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16761_length_5363_cov_4.097023, whole genome shotgun sequence.
ACCESSION   DAOPPA010000196 DAOPPA010000000
VERSION     DAOPPA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16761_length_5363_cov_4.097023"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKH54_07110"
     CDS             complement(<1..898)
                     /locus_tag="PKH54_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOC99696.1"
                     /translation="MAQSDKSRVLILFCGGTIIMKEGPDGGLKAPSRKEAVATLKAIE
                     DRLSTIADFDIHFVENIDSSNMRPTDWDNILAAIEQNYDRYDGFVVTHGTDTMAYTAA
                     ALSLAIKNLGKPVVLTGSQIPGSRLETDARRNLVNAMKLATMDVSGVFVVFDERVILG
                     SRASKASESRLDAFVSINAQDAGEIRADIHLNQSLPRRRFDKAAFFPGFDPDIAVQTL
                     TPGCDPEDLVFLLQNKRTRGIIILAYGTGNLPYDFDIFFKRAREERIPVVVTSQCLHG
                     ITRMKGYDVGLRALKLGAIEGHD"
     gene            complement(1015..2658)
                     /locus_tag="PKH54_07115"
     CDS             complement(1015..2658)
                     /locus_tag="PKH54_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99697.1"
                     /translation="MRIKKHSVPVCALLAFFAWGCAGGGGKPDADGDAVQPDIAVGDT
                     VGDVGGTSDLVADSVHDAVKDSGLPDIAQDYVEGDIDLDVAMDVALDLAIPDFGEAGF
                     VPDDDCPVECPDGTGCSDGRCVAGTLVLRQDGSGGVSGVFLTPDGVSVGFISVTDGRG
                     LARARFDLSAFDAIELGVTVTAVVPGEPGGLQVAGISWSDEMGVHLITGDGALNESDL
                     QALGRLAAGPVSKALAQVPMEVGCLPADSVDPLVVQALLTPWQFMAKYVYPDRVSRAI
                     SAASKASCIYPALQDSTWTSGSTAIRFSREAPVPHVYGLLPLDAAASAELDLVAVMPA
                     PGNEYGPCGATCRGACGADCNEISCVVTATGYACLKDENTLFNNGMRQETQEISCGTA
                     QGCRDHDDCYDLCNGQLGCGSWPAASCRRDCDIEACWLHGYETCTHWAQGAGPQDSDL
                     LTYIYPEPGSPPYEDQVNCTDPDHMTGTCWFKMQGLCGGNFCTSLDHCASLMKKDCSA
                     QSLGQFFNLKEPYDQFVVNYFSAGANCPDHCTPECVPPE"
     gene            complement(2719..3300)
                     /locus_tag="PKH54_07120"
     CDS             complement(2719..3300)
                     /locus_tag="PKH54_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HOC99698.1"
                     /translation="MSEVEMDSVLGNMVRAIAMLESSRHFAGLIPEVRVNLVYAMPGA
                     KGPGEVAAIDGRMTRVNGWPRASGLPAFGASDHMARLIVEARKYDPSIRAGINFAWTP
                     ELLAWLVARGMDPVEIDRSNEPPESAAGDGNSIPWKVAVVAASRPTFPRLFFENPAPG
                     KEPLFVLVGANALEVAARACEIAAGFLRIDANV"
     gene            complement(3293..4351)
                     /locus_tag="PKH54_07125"
     CDS             complement(3293..4351)
                     /locus_tag="PKH54_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOC99699.1"
                     /translation="MHEKDRIPFVGKLDPGSFDRIIFPRLGAPNQSLIVGPGHGVDAA
                     IVRVGNQVMAIAEDPTFGVASWGWRKFGWGIVHICAGDVAITGIEPRFMTICLMLPPG
                     TDSLVLEEIWTAIHEECVKLGITIVGGHTGSYGGIPYPLNGGCTVWGFGDPDRYVTPA
                     GARPGDAVLVTKGAAIEAAGVLALQYPKRLARAIGPDLLARAQDTYWQMSVIEDCRTA
                     FATGGVTSMHDATEGGVKGGLFEVAHASGCGISVDLRNCRVSPEAAAVCDYFGIDPFE
                     SISEGSLVLTCGQAHVQTVIAALGERGIAADVVGEMLPAGAGRTVTLADGSVTDLAFP
                     AQDPFWPAFFRTLEDPDE"
     regulatory      complement(4411..4519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4775..4948)
                     /locus_tag="PKH54_07130"
     CDS             complement(4775..4948)
                     /locus_tag="PKH54_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99700.1"
                     /translation="MALPEDVASVTGIPGSTVAVPDVPVDGRQVVETPVSSPGFPSSP
                     GGVFASSGGVLVS"
     gene            4947..>5363
                     /locus_tag="PKH54_07135"
     CDS             4947..>5363
                     /locus_tag="PKH54_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99701.1"
                     /translation="MNTTEFGASPIPPEQVGTMARGTGDCGTVGILLMSPAGSGGELT
                     RLMYQDITNIMGAGPTALPELVIDGLSPPINNASLFFAANCMATVMRPTNTGYMQYDR
                     SEGGIWAGRMVKVAGNEIVTDITTLAAWQQPDGTMKL"
BASE COUNT         1099 a         1719 c         1596 g          949 t
ORIGIN      
        1 ggtcgtgccc ctcgatggcg cccagcttca gggcgcgcag cccgacatcg taacctttca
       61 tgcgcgtgat cccgtgcagg cactgcgacg tcacgaccac cggtatgcgt tcttcccttg
      121 cccgcttgaa gaagatgtcg aagtcataag gcaggttgcc ggtgccgtag gccaggatga
      181 tgatgcccct ggtgcgcttg ttctgaagca ggaacaccag atcctccggg tcgcagccgg
      241 gggtcagggt ctgcaccgcg atatccgggt cgaaaccggg gaagaacgcg gccttgtcaa
      301 agcgtcttcg gggcagcgac tggttcaggt gaatgtcggc gcggatttcc ccggcatcct
      361 gggcgttgat tgaaacgaac gcgtcaaggc ggctttcgga cgcctttgat gcgcggcttc
      421 ccaggatcac gcgctcgtcg aatacgacaa agacaccgct gacatccatg gtcgcaagct
      481 tcatcgcgtt gaccaggttg cgccgggcat cggtttcaag gcgacttccg ggaatctggc
      541 tgccggtcag gacaacgggc ttgcccaggt tcttgatcgc cagggaaagc gcggcggcgg
      601 tgtacgccat cgtgtcggtg ccgtgggtca ccacgaaacc gtcgtaccgg tcgtaattct
      661 gctcgatcgc cgccaggata ttgtcccagt cggtcggacg catgttcgac gagtctatgt
      721 tttcgacgaa gtggatgtcg aaatcggcaa tcgtcgaaag ccgatcctcg atggccttca
      781 acgtcgccac cgcttccttc cgggaggggg ccttcaaccc gccatccgga ccttccttca
      841 tgatgatggt cccaccgcag aaaaggatca ggaccctgct cttgtcgctc tgggccatgg
      901 ccggcctccc attcggacag tccggcaatc tatacaccgg cggacggata gatcaagttc
      961 ccggcatgat ggatgaaacc cgaagatgaa atcggtcggg cagggaaccg ggggtcactc
     1021 gggtggaacg cattccggcg tgcagtgatc gggacagttc gcaccggccg agaaatagtt
     1081 gaccacaaac tggtcatacg gttctttcag gttgaaaaac tggccaaggg actgggcgct
     1141 gcaatccttc ttcatcaggc tggcgcagtg gtcaagcgag gtgcagaagt tgccaccgca
     1201 cagcccctgc atcttgaacc agcaggttcc tgtcatgtga tccgggtcgg tgcagttgac
     1261 ctgatcctcg tatggcggac ttccaggctc tggatagatg taagtcagca ggtcggagtc
     1321 ctggggcccc gcgccctgcg cccagtgcgt gcacgtctcg tatccgtgca accagcatgc
     1381 ctcgatatcg cagtcacggc gacagctggc ggcgggccat gagccgcaac caagttgccc
     1441 gttgcagaga tcgtaacaat cgtcgtgatc gcgacaaccc tgggccgtcc cacatgaaat
     1501 ctcctgtgtt tcctggcgca tgccgttgtt aaacagggtg ttttcatcct taaggcatgc
     1561 gtatcctgtg gctgtgacaa cgcatgagat ctcgttgcag tcggcaccgc aagctccacg
     1621 acaggttgca ccgcagggcc cgtattcatt tccgggggcc ggcatcacgg cgaccaggtc
     1681 aagttcggct gacgccgcgg cgtcaagcgg aagcaaaccg tacacgtggg gcacaggagc
     1741 ctcccgggaa aaccggatcg cagtcgaacc actggtccag gtcgaatcct gcagggccgg
     1801 atagatgcac gacgccttgg atgcggccga aatcgcccga gacacgcgat ccggatagac
     1861 gtacttggcc atgaactgcc acggggtcag caacgcctga accaccagtg gatccaccga
     1921 gtccgccgga agacatccca cttccatggg aacctgtgcc agcgcctttg aaacgggccc
     1981 ggccgccaac cgcccaagtg cctgaagatc ggattcgttc aaggcgccgt cgccggtaat
     2041 caagtggacg cccatctcat ccgaccagga gatcccggct acctgcaagc cgccaggctc
     2101 gcctggaacc acggctgtca ctgttacacc aagttcgatg gcatcaaatg ccgacaggtc
     2161 aaagcgagcg cgggccagac ctcgtccgtc agtcaccgaa atgaacccga ccgagacgcc
     2221 gtcgggggtc aaaaagaccc cggaaacgcc cccgcttccg tcctgacgaa gcacaagcgt
     2281 gccggcaaca cagcgtccgt cgctgcaacc ggttccgtcc gggcattcga cgggacaatc
     2341 gtcatcggga acgaacccgg cttccccgaa atccggaatg gccaggtcaa gggcgacatc
     2401 catggcgaca tccaggtcga tatccccttc gacgtagtcc tgggcaatgt cgggtaaccc
     2461 ggaatccttg acggcatcgt gcaccgagtc cgcgacaaga tcagaagtgc cgccgacgtc
     2521 gccgaccgtg tcgccaacgg caatgtcagg ctgcaccgca tcgccatctg cgtccggctt
     2581 cccgcccccg ccggcacatc cccatgcgaa gaatgcgagc agggcgcaga caggaacgga
     2641 atgctttttt atgcgcaaga ttcacccccg tcattccagg ctgggaaagc aacttaatcg
     2701 gatgtcgtca gggaattatt acacgttggc gtcgattctc aagaagcctg cggcgatctc
     2761 gcacgcgcgg gcggccactt ccagtgcgtt ggccccgacc aggacgaaca gcggctcctt
     2821 gcccggcgcc gggttttcaa agaacagcct cgggaatgtc ggacgggacg cggccacgac
     2881 ggccaccttc cacgggatgc tgttcccgtc gccggcagcg gactcgggcg gctcgttcga
     2941 tcggtcgatt tcaacgggat ccattccgcg cgcgacaagc caggcaagca attccggagt
     3001 ccatgcgaaa ttgattcctg cccttatgga cgggtcgtat ttcctggcct cgacgatcag
     3061 cctggccatg tggtcggacg cgccgaaagc cggcagtccc gaagccctgg gccagccgtt
     3121 cacgcgtgtc atgcgcccgt cgattgcggc gacttccccc gggcccttgg cgcccggcat
     3181 cgcatatacc aggttcacgc gcacctccgg aatcaggccg gcgaagtgcc gcgacgactc
     3241 gagcatggca atcgcccgaa ccatgtttcc caacacggaa tccatttcaa cttcactcat
     3301 cgggatcctc caacgtcctg aaaaatgccg gccagaacgg atcctgggcc gggaacgcaa
     3361 gatcggtgac gcttccgtcg gccagcgtca ccgtccgtcc ggcacccgca ggcagcatct
     3421 cgccgaccac gtcggccgcg atgccccgtt cgccaagcgc cgcgatcacc gtctggacat
     3481 gagcctggcc gcaggtcagg accagacttc cctcgctgat ggactcgaac gggtcgatgc
     3541 cgaagtagtc gcagacggcc gcggcctcgg gtgaaacgcg gcagttgcgc agatcaacgg
     3601 aaatcccgca tccgcttgca tgggcaacct cgaacaggcc acccttcacg ccaccctcgg
     3661 tcgcgtcgtg catggacgtc acgccaccgg tcgcaaacgc cgtccggcaa tcctcgatga
     3721 cgctcatctg ccagtaggtg tcctgggcgc gggccagcag atccgggccg atggccctgg
     3781 ccaggcgctt cggatactgc agggcaagga cgccggccgc ctcgatggcc gcgcccttcg
     3841 tcaccagcac ggcgtccccc ggccgtgccc cggccggggt gacatacctg tcgggatcac
     3901 cgaatcccca gacggtgcag ccgccgttaa gcggatacgg aatcccgcca taacttccgg
     3961 tgtgcccgcc gacgatggtg attcccagct tcacgcattc ttcgtgaatc gcggtccaga
     4021 tttcttccag aacaagcgag tccgtgcccg gtggcagcat caggcatatg gtcatgaaac
     4081 gcggctctat gccggtgatg gcgacatcgc cggcacagat gtgaacgatt ccccacccga
     4141 acttgcgcca gccccacgat gcgacaccaa acgtcggatc ctcggcaatc gccatcacct
     4201 ggttgccgac gcggacgatg gcggcatcga cgccgtgtcc gggaccgacg atcagcgatt
     4261 ggtttggcgc gccaagcctt ggaaagatga ttcggtcaaa agaccccgga tcaagcttcc
     4321 cgacgaacgg aatcctgtcc ttttcgtgca tttctgccct cctcccggtt gcgggaaatc
     4381 cagggaaggg ccggttcgac gtaaatctgt tgggaaatca tctggcctcc cttcgccggc
     4441 attacccgga tcaggttcaa tgggtcgatg cttgaagcat cctctcagcc gatacccacc
     4501 ggctccccag tcgaggccaa atgtaatacg gaccagatgg tgatgcaacc aggaaccgga
     4561 accggtggct ggaaccagag gctggaagcg ggagacgacg ccaggcgatc catgcggtgc
     4621 atgcacgccg gaatttcggc tataatccgc gaatctcgct gcggatgaaa tccatgaaac
     4681 cgatgcttcc gattctgctt gtggccgcca ttcttgccgc cggctgtggt gacgacaatc
     4741 ccggccacgg tttttacgac gacggcacgc ccatctaaga caccaatacg ccgccggaag
     4801 acgcaaacac gccaccgggc gacgacggga atcccggcga agacaccggc gtttcaacca
     4861 cttgtctgcc atcgacggga acatcgggga cggccaccgt tgaaccggga atccccgtga
     4921 cgctggcaac atcttcgggc aaggccatga atacgacgga attcggcgca agtcctatcc
     4981 cgccggaaca ggtcggcaca atggccaggg gaaccggcga ctgcggcacg gttggaatcc
     5041 tgctgatgag tcccgcagga agcggcggtg aactgacaag gttaatgtac caggacatca
     5101 cgaacatcat gggggccggg ccgaccgcac tacccgaact cgtaatcgat ggactttccc
     5161 cgcccatcaa caacgccagc ctgttcttcg cggcgaactg catggcgacc gtcatgcgac
     5221 cgacaaacac cggatacatg cagtacgacc gcagtgaagg cggtatctgg gccggcagga
     5281 tggtcaaggt ggccgggaac gagatcgtga ccgacatcac cacgcttgcc gcctggcagc
     5341 agcccgacgg aacgatgaag ctg
//
LOCUS       DAOPPA010000197         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16763_length_5363_cov_3.590806, whole genome shotgun sequence.
ACCESSION   DAOPPA010000197 DAOPPA010000000
VERSION     DAOPPA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16763_length_5363_cov_3.590806"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1275)
                     /locus_tag="PKH54_07140"
     CDS             complement(115..1275)
                     /locus_tag="PKH54_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99702.1"
                     /translation="MFTRWMTLNPERSVLLIGPRRSGKTTFLRAAFPNYRYATLDDLD
                     LLEWARRDPKGFITSLGDRGIVDEIQREPLLTVAVKYAIDNQGARILMTGSSSIGLLD
                     ASADTLAGRVDMLSMPTACWGEEFGDPTHSIFNGEPPFPVLQAGQRSLNAALSFGGFP
                     EVVAVSDPDQKLQILRNYRDTYFVRDLMQMANLGSMSGLQGILANVGRSIGSHLETSS
                     FARESGLSVPTTKKYLDTLFQSHLAFQLHGYQYGPAKRFVKAAKAYFADNGIISGLGL
                     QLSQGQILENFVISELEKRRKLGLFRADGLFYYKSQTGREVDIVFESGGVVHAIEIKA
                     TRRPTPHDATALRAFMTGLQTPSRGFVFHMGDDVFDDAGVRFLPVASIFRGA"
     gene            complement(1538..2275)
                     /locus_tag="PKH54_07145"
     CDS             complement(1538..2275)
                     /locus_tag="PKH54_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HOC99703.1"
                     /translation="MALGSDIEWTESTWNPVTGCTKVSPGCAHCYAERMARRLCAMGQ
                     PNYRNGFAVTTHPHALALPLGWRAPQVIFVNSMSDLFHPEVPDEFIHQVFNIMRRADW
                     HTFQVLTKRADRLAAMAPQLPWPPNVWVGVSVENEAFCGRIDHLRQVPAAVRFVSFEP
                     LLGSVGILNLVGINWAIVGGESGPGARPMSPEWAIAIRDQCVDAGIPFFFKQWGGVRK
                     RQAGRHLDGRLWNQWPVIPCASSSSSL"
     gene            complement(2259..3194)
                     /gene="tcmP"
                     /locus_tag="PKH54_07150"
     CDS             complement(2259..3194)
                     /gene="tcmP"
                     /locus_tag="PKH54_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOC99704.1"
                     /translation="MSTKMSSVSHSFGGDWTLTKLQILSKYLKAYNVALDGKPSLDRP
                     FRRAFIDAFAGTGYRSVGADLPEDTFRPSIPELEIVDTVRLLDGSARMALMVEPRFDK
                     YIFIEKNRDRCNELSALRTEFPDRAASIQIYEGDANEEIQKLCRANWAHHRAVMFLDP
                     YGLQVDWKTIEAIAATKAIDLWILFPLGIGVNRLLVRDGNIPLAWRRRLDSLLGTTEW
                     FDEFYKLETRNDLLGTDITQITKQGIDCIGQFFINRLSQIFPGVSRNPAILRNSTNCP
                     MYMFCFAVANPNGVKPAMKIAGDLLKKVERNGTRK"
     gene            complement(3282..4655)
                     /locus_tag="PKH54_07155"
     CDS             complement(3282..4655)
                     /locus_tag="PKH54_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99705.1"
                     /translation="MHRNALNDLIEWKDKPLRKPLVIRGARQVGKSHLVRMFAAAHFD
                     NLVEVNLETASNLADLFTSNDPRLIVDRLELQFNTTITPGRTLVFLDEIRAAPAVFAS
                     LRYFYEQMPSLHIIAAGSLLEFAPGSQGFAVPVGRIEYLHLGPMQFDEFLLAVGEQRL
                     AAFLDEWIPGRDLPDSIHQRLTDLFRRFIAVGGMPGVVAAHVQAPTHRQVEEVKHSIL
                     ATYQDDFGKYGARVNHRNVRTVFGAIPAQVGNRLKYANIDRDTRSAELSAALDLLCRA
                     RVCTKVRRTPANGVPLAYQADDTNFKALFLDVGLMNTRLGLSMLDFMRSPDLMAVNQG
                     AVCEQVVGQHLLYSAPSCQEPELYFWSRDKASSAAEVDYVIAVGPRVVPVEVKAGATG
                     RLKSLHMFLREKKASFAVRLNSDLPSLLEARTSIAAGDNTTFRLLSLPLYMVTQVRRL
                     IGQAQGS"
BASE COUNT         1229 a         1537 c         1562 g         1035 t
ORIGIN      
        1 aatcgcggta cgggaccggg cggcgacgtt tttggacgtt cgtgaagtcg agtaaagatg
       61 gcgtcctggg atttcatcag gtcggaataa gaatgctgaa cctcgccagg ttgattacgc
      121 accccggaag atcgaggcga ccggaaggaa tcggacgccg gcgtcgtcaa agacgtcatc
      181 gcccatgtga aaaacgaagc ccctggacgg ggtctgcagc ccggtcatga acgcccttaa
      241 cgctgtggcg tcgtggggcg tcggacgcct ggtcgccttg atttcaatcg catggacgac
      301 gccacctgat tcgaaaacaa tatcgacttc gcgccctgtc tgacttttgt agtaaaagag
      361 gccgtcagcc cggaacagcc ccagcttgcg gcgcttttcc aattcggaaa tcacgaagtt
      421 ttccaggatc tggccctggc tgagttgcag tcccaggccc gaaatgatgc cgttgtcggc
      481 gaagtacgcc ttggccgcct tgacgaatcg cttggcgggg ccgtactgat atccgtgcaa
      541 ctgaaaggca aggtgcgact ggaacaatgt gtccaggtac ttcttcgtcg tgggaaccga
      601 aaggccggac tcacgcgcga acgaactggt ttccagatgc gatccgattg aacggccgac
      661 gtttgccagt atcccctgca accccgacat gcttccaagg ttggccattt gcatcagatc
      721 ccgcacgaaa taggtgtccc gataattgcg cagaatctgc aacttctggt cggggtcgga
      781 aactgcgaca acttcgggaa aaccgccgaa cgacagagcc gcgttcaggc tgcgctggcc
      841 ggcctgcaga acaggaaaag gtggttctcc attgaatatc gaatgggtcg gatcgccaaa
      901 ttcctcgccc cagcaggccg tcggcatcga caacatatcg acccggccgg ccagcgtgtc
      961 ggccgacgcg tccagaagac caatggaact ggaccccgtc atcaggatcc gggcgccctg
     1021 gttgtcgatg gcgtatttga cagccacggt cagcagcggc tcgcgctgga tttcatccac
     1081 gattccgcgg tcgccaaggc tggtaatgaa accttttgga tcgcgccttg cccattccag
     1141 aaggtccaga tcgtccaggg ttgcatagcg gtaattggga aatgcggccc gcaggaaagt
     1201 tgtctttccg ctgcgcctgg gaccgatgag cagcacagac ctttcgggat tcagggtcat
     1261 ccatcgtgta aacatcttgt caccagggta actgactttt tcattttacc cgcgttaaac
     1321 ttaaaatcaa ctttcagaat aacagcggta aagtgaaaat cccgacccag aaccttacac
     1381 tgcgaataat gccggggcgt aatcaccgtc gcgcgccgcc aggccgaacc cgccagcacc
     1441 aggaaccggt cgtcaccgaa acccccaacg gcccgacctc gacattttcc agcccgaata
     1501 gcaaatagct gctatcgccg cacgcatcag caagaaatta caaagaagaa gacgatgaag
     1561 cacaggggat aaccggccac tggttccaca gacggccgtc aaggtgacga cctgcctggc
     1621 gcttgcgcac cccgccccac tgcttgaaaa agaacgggat accagcatcg acgcactggt
     1681 cgcggattgc gatcgcccac tcgggggaca tcggacgagc gccggggccc gactcgccgc
     1741 cgacgatggc ccagttgatg ccgaccaggt tcaggatacc gaccgaacca agcagcggct
     1801 cgaacgatac gaagcgcacg gcagccggca cttggcgaag atggtcgatt cgaccacaga
     1861 aggcctcgtt ctcgacgctg acaccgaccc agacgttggg tggccaggga agctgcggcg
     1921 ccatcgcggc aagacggtcg gcgcgttttg tcagcacctg gaacgtgtgc cagtcggcgc
     1981 gccgcataat attgaagacc tgatggatga attcgtcggg aacctcggga tggaaaaggt
     2041 cactcatgga atttacgaaa atgacctgcg gcgccctcca gccaagtggc aaagccaggg
     2101 cgtgcggatg ggtggtgaca gcgaaaccgt tacgataatt cggctggccc atggcgcaca
     2161 gccgacgggc catccgctcg gcgtaacagt gcgcgcaccc gggactgacc ttggtacacc
     2221 ccgtaaccgg gttccaggtc gattcggtcc actcgatatc acttccgagt gccattcctt
     2281 tccacctttt taagaaggtc gccagcaatc ttcatcgctg gttttacccc gtttggattc
     2341 gcgactgcaa agcaaaacat gtacatcgga caatttgttg agttgcgcag gattgccgga
     2401 tttctggaaa cccctggaaa aatctggctt aggcgattga taaagaactg accgatgcaa
     2461 tcgataccct gctttgtaat ctgcgttatg tcggtgccaa gcagatcgtt ccgcgtttcc
     2521 aacttgtaga actcgtcgaa ccactcggtg gttcccagaa gagaatcaag gcgacgacgc
     2581 catgcgagtg ggatattgcc atcgcgcacc agcaacctgt tgactccaat accaagggga
     2641 aacagaatcc acagatcgat cgccttggtt gccgcaatag cctcaattgt tttccaatca
     2701 acctgcagac cgtaggggtc aaggaacatg acagcccgat ggtgggccca attcgcccgg
     2761 cacagcttct gaatttcttc gtttgcgtcg ccttcataaa tctgaatgct tgcggcgcgg
     2821 tcgggaaatt ccgttcgaag ggctgacagt tcgttacaac ggtcgcgatt cttctcgata
     2881 aatatgtact tgtcgaatct gggctcaacc attagtgcca ttcgagcgga accgtccagt
     2941 aaccgcacgg tatcaacgat ttccagttca gggattgatg gacgaaacgt gtcttccggc
     3001 aaatctgcgc cgacagaacg atacccggtt cccgcgaacg catcgatgaa ggccctgcgg
     3061 aacggcctgt ccagggaagg cttgccgtca agcgcgacgt tgtatgcctt caagtacttt
     3121 gaaagaatct gcaacttggt cagtgtccag tcacccccga acgagtggct gacgctggac
     3181 atttttgtcg acatatctac ctccagccga acgaattcgg cgttgattcg aaggcttcta
     3241 tcgaccagtg ataccgcatt gtttcagatt cacgggcgga atcaagagcc ctgtgcctgc
     3301 ccgatcaacc ggcgtacctg cgtgaccatg tacagtggaa gggacaaaag gcggaatgtg
     3361 gtgttgtcgc cggcggcgat cgaggtgcgg gcttcaagca gggacggaag atcgctgttc
     3421 aggcggaccg caaaactggc cttcttttca cgcaggaaca tgtgcagcga tttcaggcgg
     3481 cccgtggcgc cggccttgac ctcgaccgga acgaccctgg gaccgactgc aattacgtaa
     3541 tcgacctcgg cggccgaact ggccttgtcc cgggaccaga aatacaactc gggttcctga
     3601 caggacgggg ccgagtacag caggtgctgg ccgacgacct gctcgcagac cgccccctgg
     3661 ttcacggcca tcaggtcagg cgagcgcatg aagtcaagca ttgacaggcc cagcctggta
     3721 ttcatcaacc cgacgtccag aaaaagcgcc ttgaagttcg tatcgtcggc ctggtaagcc
     3781 agtggcaccc cgtttgccgg cgtccgccgc acctttgtgc agacacgtgc gcggcacagc
     3841 aggtccaggg cggcagacag ctcggcggac ctggtatccc ggtcgatatt ggcatacttc
     3901 agccggtttc caacctgggc cggaatcgcg ccaaagaccg tccggacatt gcgatgattg
     3961 accctggcgc cgtactttcc aaaatcgtcc tggtatgtgg caagtatcga gtgcttgacc
     4021 tcttccacct gacgatgcgt cggggcctgg acatgggcgg ccaccacacc tggcatgccc
     4081 ccgacggcga tgaaccgtcg gaacagatcc gtcagacgtt gatgaatcga atcgggaagg
     4141 tcacggcctg gaatccattc atcaaggaaa gcggccaggc gttgctcgcc gacggccagc
     4201 aggaactcat cgaactgcat cggtcccagg tgaagatact caatcctgcc gaccggtacg
     4261 gcaaacccct gggagccagg cgcgaattca agcaatgaac cggcggcgat gatgtgcagg
     4321 ctcggcatct gttcataaaa atatcggagt gacgcaaaaa cagccggagc agcccggatt
     4381 tcgtccagga agacaagcgt cctgccaggg gtaatagtgg tgttgaactg taattccagg
     4441 cggtcaacaa tcaaccgggg atcgtttgac gtgaacagat cggccaggtt tgaagcggtt
     4501 tccaggttga cttcgaccag gttgtcgaaa tgggccgccg cgaacatcct gacaagatgg
     4561 gacttgccga cctgccgcgc gccgcggata accaaaggtt ttcgcagggg tttatccttc
     4621 cattcaatca ggtcgttcag ggcgtttcga tgcatcgcag acaccattct gctaaaatca
     4681 agggtaattt tgacagactt gacgctaaaa tcaagggtga tttaataaga cttatgttaa
     4741 aactaagggt gattttgaca ccgcagcgat caggcccccc aagatatcga ggcggccgca
     4801 aggaatcgga cgccggcatc agttgtgttt gatataccag agcggaggca gccttggaaa
     4861 gaacgccaga cacgacagaa gccaggggcg tagtgcccgg aggggccgcg ggggtacccg
     4921 taacggaacg cctcggaggc gcgcgacacc ttgcggggcc ttaatgaccg tcgcgcgccg
     4981 agagcacgag aagcgcccgt ggggcgggcc ccgcgggcga cttcgtgtcc gggacgggtt
     5041 acccccgcag gccccttagc acacaacgac gggcggacgt cagctcctcg ccccccgaag
     5101 cgaagcggat ggccccacgg gcgaattttc attcgccctt acggagcgag gggcaaagcg
     5161 gcgcgctcct gttcctattg tgaccaaccc cgattccaat taagatacgc gcgaactgaa
     5221 tcggggagtc cgggatgggc gaaaccaaca agatcgaaaa gcgcgtgatc atggatgcgc
     5281 gggccgtgga actggccgtg tcggacctgg cgggccggat tcgggccgca agcgacgagg
     5341 accaggtgtg cctgattggc atc
//
LOCUS       DAOPPA010000198         5362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16765_length_5362_cov_3.665348, whole genome shotgun sequence.
ACCESSION   DAOPPA010000198 DAOPPA010000000
VERSION     DAOPPA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16765_length_5362_cov_3.665348"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKH54_07160"
     CDS             <1..826
                     /locus_tag="PKH54_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99706.1"
                     /translation="VGQLPGQTYEGIGLDANNFKHDTFVYGAAAEIPFRQWKFAISLM
                     GMHDAETVGQTLDLMTASARISADYKWINFGLDLALQYGKTDSGAAFADETTLAWALQ
                     AFAQIKKHGFDFQLNHMMLSADDLYDRNGTNGAFFYSGKSRSRTIILSEDEIRDQGYN
                     IDEFMGQRRNTFNLLRSGFSLTDFALAYNYKDIFVPAIIVGAGFSLQPDNALGGSLVG
                     VEADLDLEIRYKDLLSFHVIGGLLVPGKAAAAFVNRGDMTSTNIQYMVESSLAVMF"
     gene            865..1695
                     /locus_tag="PKH54_07165"
     CDS             865..1695
                     /locus_tag="PKH54_07165"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC99707.1"
                     /translation="MTKDFIPACVLAFTPHADDVTLFAGGTLALWSSRGCRVHVVRVT
                     NDEKDSTRPDIPGAIERNRAEFEGAMSILGVAGVSHLGFRDCELMDVPYGVLRESFIR
                     KIREIRPEVIVSFDPAENDDENPDHHVVARAVADASWAAGYPNFHPEHAGQGLEPHVP
                     LGNFYFTRHFVRGEHVVDIASFLDTKIEAALCHENMLCTLMNDQRRRLKAAGFDFPYL
                     MGRPDSEYAEYWRMVVGASAQLAALGTDLEFAERFRHTLITEDDALVQLLSGACSPAD
                     "
     gene            complement(1809..3527)
                     /locus_tag="PKH54_07170"
     CDS             complement(1809..3527)
                     /locus_tag="PKH54_07170"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99708.1"
                     /translation="MVKRLSVIISFAFLAFSACGSDNPIVDVTEGDDINVSETTEQDI
                     AGEDIVEDTGRTDTVTDLGYLDTNQPDTNQPDMNQPDTNQPDMNQPDMNQPDMNQPDD
                     NPIEDLPVEDVPVEDIPVEDVPVEETEMPWMCAVDDDCADYFDDADYDQCHRPACDTE
                     SGECVIIPVDDGTECSDGEFCTAPDTCVAGECIPGGMVTCDDGDACTFDECVGGACVN
                     TPDTTILTCGIGPCYREIPACEAGVVNECVPGEPQAEICDTIDNDCNGFADDNLTDTG
                     AACDSDLLGPCLAGTVACVEGVLSCVPNVQPTAEKCDKQDNDCDGVIDNDPVSNGMPC
                     AVPGQLGNCQVGETGCTNGLVVCVQTNFPEAEKCDGIDNDCDNDIDENSPGAGVACST
                     GLPGICNDGTTSCATGKLECTQTTFPQTEICDGLDNDCDGVTDPANTTGCTTFFKDVD
                     GDGFGSAETACLCAAVAPFNTIVGGDCCDTDSRAKPVGAAPYTTQNNCNSWDYNCDGT
                     VTRQYNYGDGSCGGLLFCKQDMVEGWKGAAPNCGEVGTFLENCTLEFIPPVCQETTKK
                     LTQGCF"
     gene            complement(3615..4760)
                     /locus_tag="PKH54_07175"
     CDS             complement(3615..4760)
                     /locus_tag="PKH54_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99709.1"
                     /translation="MDELRVLVVDDELGIRMAIAKALRNVVVEVPEFKSSFALVTDSA
                     ETAEDAYEMVKQNPPDILLLDQKLPGMSGIELLEKLVAEQVEVLTIMITAYASLETAV
                     AATKSGAFDFLAKPFTPYELRACLFKAAKHIVLQRQAMKLVAERKRVRFEFLSVVAHE
                     LKSPTAAVEGYLMMMKDRVMGDTLDDYTDVMDRAIFRLSGMHKLIADLLDLTRIESGS
                     KRRDVVDVDVLRIADLSADSGRELGKRRGITVDVHKDGETTMKADSGEIELIINNFVS
                     NAVKYNRDQGHVDVTVGRTPDGMMRIQVRDTGIGMKPEDVKRLFGEFVRIKNEKTKRI
                     EGSGLGLSIVKKLVDLYQGRIEVHSEWEVGTQFTVFIADADPAIQES"
     gene            complement(4790..5206)
                     /locus_tag="PKH54_07180"
     CDS             complement(4790..5206)
                     /locus_tag="PKH54_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC99710.1"
                     /translation="MSEQMRNGNGRRILIVDDDVDFLDITEARLKADGFTVIRCEGQA
                     AAEEFLSKGEPFDLAIVDLMMEFMDSGFVISRKIKKIDATRPVIMATGVEAETGLGFD
                     AATREERSWIQADVMLEKPLRYDQIRKHIDMLLPVE"
BASE COUNT         1002 a         1592 c         1563 g         1205 t
ORIGIN      
        1 ggtcggccag cttccaggcc agacctatga aggtatcggg ctggatgcca acaacttcaa
       61 gcacgacact ttcgtctatg gagcggccgc ggagattcct ttcaggcagt ggaagttcgc
      121 catttccctg atggggatgc acgacgccga gacggtcggg cagactctcg acctgatgac
      181 cgccagcgcg cggatttctg ccgactacaa gtggatcaat tttggcctcg atctggcgct
      241 tcagtacggc aagacagaca gcggtgccgc cttcgccgac gagacgactc tggcatgggc
      301 actgcaggcg ttcgcccaga tcaagaagca cggcttcgac ttccagctca accacatgat
      361 gctttccgcc gacgatctgt acgaccgcaa cggcacaaac ggcgcgttct tctactccgg
      421 caagagccgc agcaggacga taatcctctc cgaggacgaa atccgtgacc agggatacaa
      481 cattgatgaa ttcatgggcc agcggcgcaa caccttcaac cttctgcggt cggggttcag
      541 cctgaccgac tttgcgcttg cctacaacta caaggatatc ttcgttcccg cgattatcgt
      601 cggcgccgga ttctcgcttc agccggacaa cgcgcttggc ggcagtcttg tcggtgtcga
      661 ggctgatctt gatcttgaaa tcaggtacaa ggatcttctt tcgttccacg tcatcggcgg
      721 attgctggtg cctggcaagg ccgctgccgc gtttgtgaac cgtggcgaca tgaccagcac
      781 caacatccag tacatggtgg aaagttcgct ggcggtgatg ttctaggact tgccgtttgt
      841 tttgttgggg tggaggacac gattttgaca aaggatttca ttcccgcatg cgtgcttgca
      901 tttactccgc acgccgatga cgtcacgctt tttgctggcg gcaccctggc gttatggtcg
      961 tcgcgggggt gtcgcgttca tgtcgtaagg gtcaccaacg acgaaaagga ctccacccgg
     1021 cccgacatcc cgggggccat cgaaaggaac cgcgccgagt tcgagggcgc gatgtcgatc
     1081 ctgggcgtgg ccggggtttc gcatctgggt tttcgcgact gcgaactgat ggacgtgcca
     1141 tacggcgtcc ttcgggaaag tttcatccgc aagatcaggg agatcaggcc ggaagtaatc
     1201 gtgtcgttcg atcccgccga aaacgatgat gagaacccgg atcatcacgt tgtcgcccgg
     1261 gcggtggctg acgcaagctg ggccgccggc tatccaaact tccatccgga gcacgccggc
     1321 cagggccttg agccgcacgt tccgctggga aatttttatt tcacgcgcca tttcgtcagg
     1381 ggtgaacacg tcgtcgatat tgcttcgttc cttgacacca agattgaggc ggctttgtgc
     1441 cacgaaaaca tgctttgcac cctgatgaac gatcagaggc gcaggctgaa ggcggcaggc
     1501 ttcgattttc catacctgat gggacgcccc gattcggaat acgccgaata ctggcgaatg
     1561 gttgtcggcg cctccgcgca actggccgca ctcggcacgg atctggagtt cgccgagagg
     1621 tttcggcaca cgcttatcac tgaagatgac gcgctggttc agttgctctc cggtgcgtgc
     1681 tcaccagcgg actgatgtaa ttttctccaa ttcgcccgca cgttgaacgg ttccgctcaa
     1741 ggtgattatc gacaggcaac ctgatcaatc atgtggcctg aggttcagga aaggttgaag
     1801 agggtggatc agaagcagcc ctgggtcagc ttcttggtgg tctcctggca gactggcgga
     1861 atgaactcga gggtgcagtt ttccaggaag gttccgacct cgccgcagtt cggcgccgcg
     1921 cccttccagc cctcgaccat gtcctgcttg cagaacagca gcccgccaca ggaaccgtcg
     1981 ccgtagttgt actggcgggt aaccgtgcca tcgcagttgt agtcccagga gttgcagttg
     2041 ttctgagtgg tgtacggagc ggccccgacc ggcttggccc tgctgtcggt gtcgcagcag
     2101 tcgccgccga cgatcgtgtt gaacggtgcc accgcggcgc acaggcaggc cgtttcggcg
     2161 ctgccgaatc cgtcgccgtc cacgtccttg aagaacgtgg tgcagccggt ggtgttggcc
     2221 ggatccgtca cgccgtcgca gtcgttatca agaccgtcgc agatttcggt ttgcgggaaa
     2281 gtggtctggg tgcattcgag cttgccggtt gcgcaactgg tcgtgccgtc attgcagatt
     2341 ccagggagtc cggtcgagca ggcgacgccg gcgccggggc tgttttcgtc aatgtcgttg
     2401 tcgcagtcgt tgtcgatgcc gtcgcacttt tccgcttccg ggaagttggt ctgtacgcat
     2461 acgaccaggc cgttggtgca accggtttca ccaacctggc agttgccaag ctgtccggga
     2521 acggcgcagg gcattccgtt gctgacggga tcgttgtcga tgacgccgtc acagtcgttg
     2581 tcctgcttgt cgcacttctc ggcggtcggc tggacgttcg ggacgcagga caggacgcct
     2641 tccacacagg caacggtacc ggccaggcac gggcccagaa ggtcgctgtc gcaagcggcg
     2701 ccggtgtccg tcaggttatc gtcggcaaag ccgttgcagt cgttgtcgat ggtgtcgcag
     2761 atttccgcct ggggttcgcc cggaacgcat tcgttgacca cgccggcttc gcaggcggga
     2821 atttcacgat agcagggacc gatgccgcag gtaaggatgg ttgtgtcggg cgtgttgacg
     2881 caggcgccgc cgacgcattc atcgaaggtg cacgcatcgc cgtcatcgca ggtgaccatg
     2941 ccgccgggaa tgcattcgcc ggcaacgcag gtgtcggggg cggtgcagaa ttcgccgtcg
     3001 gagcattcgg tgccgtcgtc aaccgggatg attacgcatt caccggattc ggtgtcgcaa
     3061 gccgggcggt ggcactgatc gtagtcggca tcgtcgaagt aatcggcgca gtcgtcgtca
     3121 acggcgcaca tccagggcat ttcggtctct tcaaccggta cgtcctcgac cgggatgtct
     3181 tcaaccggta cgtcctcgac cgggagatcc tcgatcgggt tgtcgtccgg ctggttcatg
     3241 tccggctggt tcatgtccgg ctggttcatg tccggctggt tcgtgtccgg ctggttcatg
     3301 tccggctggt tcgtgtccgg ctggttcgtg tcaaggtaac caagatcggt caccgtgtcc
     3361 gtccgccccg tgtcctcgac gatatcctcg ccggcaatgt cctgttccgt cgtttcggag
     3421 acattgatgt cgtcgccctc ggtcacatcg acaatcgggt tgtcggaacc gcatgcagaa
     3481 aacgccagaa acgcgaaact gattatcaca ctgagacgct tcaccataaa acgctccgat
     3541 aaagagacgc cctattctat aaggtggtca tgcaaaagtg caaatttgca gcagaaacgg
     3601 aattcgtaag gcgttcagga ctcctggatg gccggatccg cgtcggcgat gaataccgtg
     3661 aattgggtgc cgacttccca ttcgctgtga acctcgatgc ggccctggta aaggtcaacc
     3721 agcttcttga cgatcgaaag cccgagaccg gaaccctcga ttctcttagt cttctcgttc
     3781 ttgatcctga cgaattcgcc gaagagacgc ttcacgtcct ccggcttcat tccgatgccg
     3841 gtatccctga cctggatcct catcattccg tccggggttc tgccgaccgt cacatcgaca
     3901 tggccctggt cgcggttgta cttgactgca ttcgacacga agttgttgat gataagctct
     3961 atctcgccgc tgtcggcctt catggtcgtt tctccgtcct tatggacatc gaccgtgatg
     4021 ccgcgcctct tgcccagttc acgtccggaa tccgccgaca gatctgcaat tcgcagtaca
     4081 tcgacatcga cgacatcgcg cctcttgctt ccggactcga tcctggtcag atccagaagg
     4141 tcggcaatga gcttgtgcat gccggacagg cggaagatgg cgcgatccat cacgtcggtg
     4201 tagtcgtcca gcgtgtcgcc catcacgcga tccttcatca tcatcaggta tccctcgacc
     4261 gcggccgtcg gggatttcaa ttcgtgcgcg acgacggaca gaaactcgaa gcggaccctt
     4321 ttcctttcgg cgaccagctt catggcctgc cgctgaagga cgatgtgctt ggcggccttg
     4381 aacaggcaag cgcgcagctc gtatggggtg aagggctttg ccagaaaatc gaacgccccg
     4441 ctcttggtgg ccgcgactgc cgtttcaagc gacgcatacg cggtaatcat tatggtaagg
     4501 acttcgacct gctcggcgac cagcttctcg agaagttcga ttccgctcat tcccggcagc
     4561 ttttgatcca gcaggagtat gtccggcgga ttctgcttca ccatttcata ggcgtcttcg
     4621 gccgtctccg ccgaatccgt cacgagggca aaactggact tgaactccgg cacctcgacg
     4681 accacgttgc gaagtgcctt cgctatggcc atcctgatgc caagctcgtc atcgacaacc
     4741 agtactcgaa gctcgtccaa gatatacctc acgcaagggc gggcgaagtc tattccacgg
     4801 gaagcagcat gtcgatgtgc ttcctgatct ggtcgtaacg aaggggcttc tcgagcatca
     4861 cgtccgcctg gatccacgac cgttcctcac gcgtggcggc atcgaacccg agtccggtct
     4921 ccgcctcgac cccggttgcc atgatgaccg gccgtgtcgc gtcgatcttc ttgatcttcc
     4981 ggctgattac gaacccgctg tccatgaatt ccatcatcag atccacgatg gcaaggtcga
     5041 aaggttcgcc cttcgaaaga aactcttccg cggctgcctg cccttcgcac ctgatcaccg
     5101 taaacccgtc agccttcagg cgtgcctcgg tgatgtccag gaaatcgacg tcatcatcga
     5161 caatcaagat ccttctgcca ttcccgtttc tcatctgctc gctcattttc ctcacctttt
     5221 cacacgttgt tcccgtacct gggcagcgtg ataaagaaag ttgtcccggt cggcccgcga
     5281 tcgggatcca cattcgattc aaacctgatg tcgccacgat gcatcttgac gatgccgtag
     5341 cttaccggca gtcccagccc gg
//
LOCUS       DAOPPA010000199         5351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16812_length_5351_cov_3.553814, whole genome shotgun sequence.
ACCESSION   DAOPPA010000199 DAOPPA010000000
VERSION     DAOPPA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16812_length_5351_cov_3.553814"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKH54_07185"
     CDS             <1..255
                     /locus_tag="PKH54_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99711.1"
                     /translation="NREKKRIVTKGVRDLVSRGGWDTRRFESGTTGIVESARNEIRDA
                     VVDALYSQAGLCRDCLDARQKTGDPTALCDRHMNEALGMT"
     gene            complement(258..2027)
                     /locus_tag="PKH54_07190"
     CDS             complement(258..2027)
                     /locus_tag="PKH54_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99712.1"
                     /translation="MKSFAVPAIMLLSLLVACGNGDNPSVDVVGDLPVGVDTIFDTNP
                     GDTGSDSVMDEGSGDAIDDAVDDAADNGPSDAQTDVPPEIWYPPVTPPLPFGAENRGY
                     VHMNGIVHLHSRWSHDGCFPDHEEITSPEIEACSTTYVEEADINACRDAIIESMLVAC
                     VDEYRLAACAAGMEFLFQTDHPGDVRDQTFENALHYRPDSGDQMVLDAQDRQVANRMK
                     CPAESPVDHVFVYFGTEGSKQMPIGMAAPIPDIVFNTSFEDSTPLEQAQAALAAVHAQ
                     GGWGLVCHPEQKDLSVQRIVELPLDLIEIFNFHPALMGILEDFEILFQLDRFMASDED
                     APNPDLAMMLVLKPVVNDPLKFDESVKSIRLGHILATDIHRNVELPALCESAETCGDN
                     WIETYPNLARLLAKGGPAILGDGDRFDSYRRSLRWASNHALVSSTGTDYLAIRDAVGL
                     GRSYSAFDIMGIPQGFDFFAVVDGAALEMGQEATGYTDAKIYFRTPSITKPLWATERF
                     SDPADTEIVTKLIKIDADGGSRVVREVIGQGWTFEHVVDGPAVYRIEVWMTPKHLATD
                     LGDMAWVADKQYPWIYSNALFFR"
     gene            complement(2171..2701)
                     /locus_tag="PKH54_07195"
     CDS             complement(2171..2701)
                     /locus_tag="PKH54_07195"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOC99713.1"
                     /translation="MFDVIGGALLVFAMRVCDVSLGTVRQIFAVRGHGVIAAGIGFVE
                     VTIFILAISQVLTSIKDNPVYIFAYSGGFATGTLVGVYVEHLIGLGNRMIRVITVRQN
                     DAVVNNLRNRGFGVTQIDGQGKDGKVYLLLSNVRRKRVPEFLQVLKEHAPQAVYTIEE
                     VRYAHGGVLDLVRKGK"
     gene            2816..4939
                     /locus_tag="PKH54_07200"
     CDS             2816..4939
                     /locus_tag="PKH54_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOC99714.1"
                     /translation="MEHQGSNSKLLPENAYRKLNAGEEYKPIVGPAAGMTEVSRWSLT
                     MAVIMVVIFTAACVYIALRAGSGIEAAIPIAVAAIFFGRLKKVKSSILENVIVQSIGQ
                     ASGVVAAGAAFVIPALYINQVKPEWWQIFLACFIGGTLGVVLIIPLRKFFVAERHGEL
                     PFPEATAATEILVSGESSGGGAGKILLASFGLGAAYDFMVEVVHAWNPALSTKVVMGR
                     AGEWLYNFRIELKMQAIAALFGLGYIIGLKYAAIIFAGSVLGYVVMVPLIYLVGGGAG
                     AFTFAGVSYNVAAMSAGEIFAAFVKPIAIGAIATAGIIGLIRMGKIIAKSVSLGFKSI
                     GRRGGPAIVAERTNLDLAPATVLLIQGISTLAMGVLFFIVCMTVGGYSVGQSALFAVI
                     GMAIAGLLTFLFTPVAAEAIAIVGTNPVSGMTLVTVIISSLVMAMVGLKGSAGIFVAL
                     IIGAAVCTALSVSGALISDFKIGYWIGATPRNQQKWKFLGLAIASLVVAFVIPVMDQG
                     YSFLIEQNGVMVPNQDVLPAPQANMIAAVVNGLMQGGQQPYLLYGLGGLVAILAYLAG
                     IPPLAFALGMYLPISINFATLAGGFMAWIVGRSGKTDAEKQSRAGQGTLIASGMMAGA
                     AICGIVAAVLRLDWTGYAIRFLSVGEKFTIGKAASGAEVLNGEAAAWYAGFEGQLLGF
                     AMFVGLMIATFLLARWGARMEMKGK"
     gene            5076..>5351
                     /locus_tag="PKH54_07205"
     CDS             5076..>5351
                     /locus_tag="PKH54_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOC99715.1"
                     /translation="MNCTFSREAAIELLHRHVKDPNMIKHCLASEAVMRALATRVGED
                     PVKWGMAGLLHDLDVESHNDLSVHTTETVRILNGLGIDTEIVDAIRLH"
BASE COUNT         1135 a         1612 c         1528 g         1076 t
ORIGIN      
        1 aaccgcgaaa agaaacgtat cgtcacaaag ggcgtgcgcg accttgtctc gcgcggcggc
       61 tgggacacgc gacgctttga atccggaacg accggaattg tcgaatccgc ccgcaacgaa
      121 atccgtgacg ccgtggtcga tgctctttac tcccaggccg ggctctgccg ggactgtctg
      181 gacgccagac aaaaaactgg cgacccgacc gccttatgcg acaggcacat gaatgaagcc
      241 ctgggtatga cctgaaatca gcggaagaac agcgcgtttg aataaatcca ggggtattgc
      301 ttgtccgcca cccaggccat gtcgccaaga tcggtcgcca ggtgtttcgg ggtcatccag
      361 acctcgatgc gatagactgc cggaccgtca acgacgtgtt cgaacgtcca gccctgccca
      421 atgacttccc ggacaacgcg ggaaccgccg tcggcatcaa tcttgataag cttcgtcacg
      481 atttcggtat ccgcgggatc ggaaaacctt tcggtcgccc ataacggctt cgtgatggac
      541 ggggtcctga aatagatctt cgcatcggtg tatccggtcg cctcctggcc catttccagc
      601 gccgccccgt caacaacggc gaagaaatca aatccctgcg gaatgcccat gatatcgaac
      661 gccgagtatg aacgccccag tccgacggca tcccggatgg ccaggtaatc ggttccggtc
      721 gaagacacca gcgcgtggtt cgaagcccat cgaaggcttc gccgatagct gtcgaaccgg
      781 tctccgtcgc caagaatggc cgggccaccc tttgcaagca gccgggccag gttcggataa
      841 gtctcaatcc agttgtcgcc gcaggtctcg gccgactcgc acagggcagg aagttccacg
      901 ttcctgtgaa tatcggtcgc aagaatgtgg cccagcctga tactcttgac tgactcgtca
      961 aacttcaacg ggtcgtttac gaccggcttc aggacaagca tcatcgcaag atccgggttt
     1021 ggcgcgtcct cgtcgctggc catgaaacgg tcaagctgga aaaggatttc gaagtcctcc
     1081 agaatcccca tcagcgccgg atggaagttg aagatctcga taagatccag cggcagttcc
     1141 acaatccgct gaaccgagag gtccttctgt tccggatgac agaccaggcc ccagccaccc
     1201 tgggcatgaa cggcggcaag cgctgcctgg gcctgctcca gcggcgtcga atcctcgaaa
     1261 ctggtgttga acacgatatc cggaatcggg gcggccatgc cgatcggcat ctgcttgctg
     1321 ccctcggtgc caaaatacac aaagacgtga tcgacaggcg attcggccgg acatttcatc
     1381 cggttggcaa cctgccgatc ctgggcatca agaaccatct ggtcgccgga atccggccgg
     1441 taatgaagcg cattctcgaa cgtctgatca cgaacgtcgc ctggatgatc ggtctggaaa
     1501 aggaattcca ttccagcagc gcacgcggcc aggcggtatt catcgacaca ggccaccagc
     1561 atgctttcga taatcgcatc cctgcaggca ttgatgtcgg cctcttcaac ataggttgtc
     1621 gagcaggcct cgatttcggg ggacgtgatc tcctcgtgat ccgggaaaca tccgtcatgc
     1681 gaccagcggg aatgcaggtg cacaatgccg ttcatatgga cgtatccacg gttctcggct
     1741 ccgaaaggca gcggaggcgt caccggcgga taccagattt cgggcgggac atctgtctgg
     1801 gcatcggacg gtccgttatc ggcggcatca tcaacggcat catcaatggc atccccggaa
     1861 ccctcatcca tcacggaatc ggaaccggtg tcgccgggat tagtgtcaaa aatcgtatcc
     1921 acaccaaccg gaagatcgcc gacgacatcg acggacggat tatccccgtt gccgcacgcc
     1981 acgagcaacg aaagcagcat gattgcagga acagcgaatg acttcatctt ccacctctcc
     2041 agatttcaca caagacggaa aatccaggct cccagggaag gatgccagac ttctttccgg
     2101 cattatgctc ccaaaatgac acacattcga aagatacctg gcagaagtcg agaaaaatcg
     2161 accactccgg ctactttccc tttcgaacca ggtcaaggac gccgccgtgg gcgtaccgga
     2221 cttcctcgat tgtataaacg gcctgcgggg catgctcctt aagcacttga aggaactcgg
     2281 gaaccctctt gcgccggacg ttgcttaaaa gcagatagac cttgccgtcc ttgccctgac
     2341 cgtcgatctg ggtcaccccg aaaccacggt tgcgcaggtt gttgaccacc gcgtcgttct
     2401 gcctgaccgt gatgacccgt atcatcctgt ttccaagccc gatcaggtgc tcgacatata
     2461 caccgaccag cgtgcccgtc gcgaaccccc cggagtacgc gaatatatac accgggttat
     2521 ccttgattga ggtaagaacc tggctgattg ccaggatgaa aatcgtaacc tccacgaacc
     2581 caatcccagc cgcaatcacg ccatggccgc gaaccgcgaa aatctgacgc acggttccaa
     2641 gtgaaacgtc acaaacccgc atcgcgaaga ccagcaatgc gccaccaatc acgtcaaaca
     2701 tcgaaaatct ccactcacga caagaccact tccagcgcca cggaaccgaa tcctcgacac
     2761 gggaacaata aatatgctat ttccaatccg ttttgaaaac ccccggaggg cgttgatgga
     2821 acaccagggt tcaaacagca agctgcttcc cgaaaatgct taccgcaaac tgaacgccgg
     2881 cgaggaatac aagccgatcg tcgggccggc ggcaggcatg accgaagtct caaggtggtc
     2941 gctgaccatg gcagtcatca tggtggtgat cttcaccgcg gcctgcgtgt atatcgcgct
     3001 gcgtgcgggc agcgggatcg aggcggccat cccgatcgcg gtcgcggcca tcttcttcgg
     3061 gcggctcaag aaggtcaaat ccagcattct tgaaaacgtc atcgtccagt cgatcggcca
     3121 ggcctcgggc gtcgtggcgg ccggcgccgc gttcgttatc ccggcgcttt acatcaacca
     3181 ggtcaagccc gaatggtggc agatattcct ggcctgcttc atcggtggaa ccctgggcgt
     3241 cgtcctgatc attccgttgc gaaagttctt cgtcgccgaa cgccatggcg agcttccgtt
     3301 cccggaagcg accgccgcaa ccgagatcct tgtttcgggc gagtcgtccg gcggtggtgc
     3361 cggaaagatc ctgcttgcat cgttcggcct tggggccgcc tatgacttca tggtcgaggt
     3421 cgtccacgcc tggaacccgg ccctttcgac caaggtcgtc atgggtcgcg cgggcgaatg
     3481 gctttataac ttccgaatcg agctgaagat gcaggccatc gccgcattgt tcgggcttgg
     3541 ttacatcatc ggactgaagt acgcggccat cattttcgcc ggatcggttc ttggttacgt
     3601 cgtgatggtt ccactgattt atctggtcgg cgggggagcc ggggcgttca cgttcgcagg
     3661 cgtcagctac aacgtcgcag cgatgtcggc cggtgaaatc ttcgccgctt tcgtcaagcc
     3721 gattgcgatc ggcgcaatcg ccacggccgg catcatcggc ctgatccgca tgggaaagat
     3781 tatcgcgaag tccgtcagcc tcgggttcaa gagcatcggc aggcgcggcg gaccggcaat
     3841 cgtcgccgaa aggaccaacc tggatctggc ccctgcgaca gttcttctga tccagggaat
     3901 cagcacgctt gccatgggtg tcctgttctt catcgtatgc atgaccgtcg gcggttacag
     3961 cgtaggtcag tcggcgcttt tcgcggtgat cgggatggcc attgccggac tgctgacgtt
     4021 cctgttcacg cctgtcgcgg ccgaagcgat cgcaatcgtc ggaaccaacc ctgtttcggg
     4081 aatgacgctg gtcaccgtta tcatcagctc actggtgatg gctatggtcg gactgaaggg
     4141 atcggccggc atcttcgtcg ccctgatcat aggcgccgcc gtgtgcacgg cgttgtcggt
     4201 atcgggcgcc ctgatttccg acttcaagat cggttactgg attggcgcga cgccgcgcaa
     4261 ccagcagaag tggaagttcc tgggactggc aatcgcgtcg ctggttgtcg cattcgtcat
     4321 tcctgtcatg gatcagggat atagtttcct gattgaacag aacggcgtta tggttccgaa
     4381 ccaggacgtg ctgccggcgc cgcaggcgaa catgatcgct gcggtcgtca acggactgat
     4441 gcagggcggg cagcagccat acctgctgta cggacttggc gggctcgtcg ccatcctggc
     4501 atacctggcc ggaattccgc cgcttgcgtt cgcccttggc atgtatctgc cgatttccat
     4561 caacttcgcc acgctcgccg gcggcttcat ggcctggatc gtcggacgca gcggaaagac
     4621 cgatgctgaa aagcagtccc gtgccggaca gggcaccctt attgcatccg gcatgatggc
     4681 cggcgccgca atctgcggca tcgtggcggc ggttcttcgc ctggactgga ccggatacgc
     4741 aatcaggttc ctgtccgtcg gcgaaaaatt cacgatcggg aaggctgcca gcggcgccga
     4801 ggtccttaac ggggaagccg ccgcatggta tgccggattc gaaggccagc ttcttggatt
     4861 cgcgatgttc gtcggcctga tgatcgccac gttcctgctt gcccgctggg gtgccaggat
     4921 ggagatgaag ggcaaatagc cacgtttcca gtggcccgat cgagcatatt gaaaccaatc
     4981 gacgcttttg cggatgcgct tgaacaggag cccttttccg tgacccgcgc caactcattg
     5041 gctccgtccc aatcaccttg cgcaatgagg ttgagatgaa ctgcacattt tcgcgcgaag
     5101 cggccatcga actgctgcac cgtcacgtca aggatccgaa catgatcaaa cactgccttg
     5161 cgtccgaggc ggtcatgcga gcgcttgcga cccgagtcgg ggaagacccc gtcaaatggg
     5221 gcatggccgg actgctgcac gatctggacg tggaatccca caacgacctg tcggttcaca
     5281 ccaccgagac tgtgcgaatc ctgaacgggc tggggattga cacggaaatc gtcgatgcga
     5341 tccgcctgca c
//
LOCUS       DAOPPA010000200         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16836_length_5347_cov_4.286659, whole genome shotgun sequence.
ACCESSION   DAOPPA010000200 DAOPPA010000000
VERSION     DAOPPA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16836_length_5347_cov_4.286659"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2969
                     /locus_tag="PKH54_07210"
     CDS             <1..2969
                     /locus_tag="PKH54_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC99716.1"
                     /translation="VVPYPGASPEEVESQVTRPIEDVLYGINDVDEVISYSRDSVSQI
                     VVTFKMTADPQQASNDVRDRLAMIRGTLPREIMDPLISRFDPNSSPVLTYMVSGPGTS
                     AQTRRMVEDIITPALQRVEGVGNVGVTGGDQREIRVEIDRTRLDGTPIGLAQLAQLLG
                     AEAIDVPGGRVTSDQGEISVKAVGRFSSPEDIGDVVLMGLPNGAQLRVRDVADVVDGV
                     KEVRTISRVDSTTGVSFVVFKQATANTVDVVRKVEKALEGVSGQIPKDFKIQKIVDNS
                     TFTEATYVELRQALILGAIFAIFVIFLFMLDWRSTVISAIALPISVIGTFFVIWQLGF
                     SLNLISMISLSLAIGMLIDDSVVVRENIFRHLERGEDPFTAAHKGTAEIALAVMATTF
                     TIVAVFVPIAFTAGLVGRFLKEFGITVAAAVLVSLVVSLTVDPMLSARLVQDIPPDYH
                     QKRRQHKFFGFFIRGYDALDSTYRRMLLWAISHRKTVVIAAIALFAGSLGLVQLMGTQ
                     FIPRGDQSAFSMNLELPAGTPLSEMDRVTAIVEGMVDELPEVTTISTTVGPDEAVNRA
                     YIYVLTTPRNERDRSVTDMMEVLRPRLAAIPGLVYSLNDADIFGQSFALMSAAFTMRI
                     RGSDYQELGRIANEAFEMVKSTTGIRDAAISFRPGMPEKSFVVDRKRSGDMGVVFAQA
                     AMTLRIGLTGAPVARFRDGSRDIDVRVQLRPEDRADVESVLDLKVPTNRGGMVGLRDV
                     VNVKDTVLPATIERQNRERYVTITANLAGRPLGSVIKDLKPKLDTIQKPDGYRIEFAG
                     DAEMMDETFANMALALILGVVFIYFVLASQFESLVHPLTIMLSLPLALVGALVTLFLF
                     GISLDIIAMIGIVLLMGLVTKNAILLVDYTNQLREKGLSITDALLEAGPTRLRPILMT
                     SAAMVLGMLPTAIGTGAGAEFRVPMSISVIGGVISSTFLTLIVVPVVYIWMDRFTIKG
                     RRLQKRAEAATFDVDE"
     gene            2990..>5347
                     /locus_tag="PKH54_07215"
     CDS             2990..>5347
                     /locus_tag="PKH54_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOC99717.1"
                     /translation="MTDGRLVKARFPAFVLVFIAAASGCGDKPFPFDTGGGDLPADIS
                     PEVAISDLAIRFTTPAPESRPVVSGIYQVVFDYSDPEGKPATLTAGFYKHPEIQPYVI
                     QADGPGTYSINVDTTLVEDGNRSVEIIALSSDGRTVSKNGKILVDNNRPEIEILEPTP
                     AAGAGFVDDLSFRVRVTDSGSKVHFVRVTIGQFTWEWIPQDGEPAAEVDTRIVEDAAK
                     GIYIDVVVPTEGWRGADLVMKVVARDGVAGRDAVVEHPLVFVERPGLDGGERVFLPLV
                     ENDALESVEGIRLGPADDGDWAVLGRVRKKVGYELVLFQRSTPGHAVQVGTVLAEECQ
                     IHKIFDMNIDGMDDILAWCGLGASRRIVVFLQGADRAFVAADSYPTEHMVKDLAAGDL
                     NRDTFPDIAFVSDVQPLWTGIMLSITDQYGDFLGYGPPSFYSGAVRPTHVAIGRFSDD
                     QNNAVVVGASGSGLVTAYPIDESGVPSMGENSSLDPDNLLIDDVSDMVAVNFGKTSQD
                     NDALVVVDSRNVEVLGVARLRQDASRRVESVHEWPTGAMPTDIAAGDIDVDGAPDIAV
                     LCGGSNMVDVFMGVREGGYYEPVSDGRPLLVGSATDVTLADMDFDGYLDVVALTGKRE
                     MKVVYYRKSAAGGWMAGSRQALVPAAPISLAVGHFVEPLSGTRAGFLDAAVLYMLPDN
                     RGPALTVASAEAVSGQPVSFAGELLLSPKYPTGLVAGNFDIDLNGGGATSLTRLDDLI
                     VTSDDTISPQNPHTAWAILFQEAGHNSAVAQTVGIFVDKSPMYAAV"
BASE COUNT          958 a         1554 c         1720 g         1115 t
ORIGIN      
        1 tggttgtccc gtatcccggc gcaagcccgg aagaggtcga gtcccaggtt acccgtccga
       61 tcgaggacgt tctttatggc atcaacgacg tcgatgaagt aatcagttat tcgcgggatt
      121 cggtctcgca gattgtcgtc accttcaaga tgacggccga tccccagcag gcgtcgaacg
      181 acgtccgcga ccgcctggcc atgattcgcg gcacgcttcc gcgcgaaatc atggatccgt
      241 tgatttccag gttcgacccc aattcttccc cggtgctgac gtacatggtg tccggccccg
      301 gcacgtcggc ccagacccgc cgcatggtgg aagacatcat cacgcccgcg ctgcagcggg
      361 tcgagggcgt cggcaacgtc ggcgtgaccg gcggcgacca gcgcgagatt cgcgtcgaga
      421 tagaccgcac caggcttgac gggacaccga tcggcctggc ccagcttgcc cagttgctgg
      481 gggccgaggc catcgatgtt ccgggcgggc gcgtcacgtc cgatcagggc gaaatcagtg
      541 tcaaggcggt cggacggttc agcagccccg aggacatcgg cgacgtcgtc ctgatgggtc
      601 tgccgaacgg cgcccagctt cgggttcgcg atgtggccga cgtggttgac ggcgtcaagg
      661 aagtccgcac gatttccagg gtcgattcga cgacgggcgt ttcattcgtg gtgttcaagc
      721 aggcgacggc caatacggtc gatgtcgtcc gcaaggtcga aaaggcgctt gaaggcgttt
      781 cgggccagat tccgaaggac ttcaaaatcc agaagatcgt cgataactcg accttcaccg
      841 aggcgaccta tgtcgaattg cgccaggccc tgattcttgg cgccatcttc gccatcttcg
      901 tgattttcct tttcatgctc gactggcgct cgaccgtcat ttcggcaatc gcactgccga
      961 tttcggtcat cggcaccttt ttcgtgatct ggcagctcgg gttttccctg aacctgattt
     1021 cgatgatttc cctgtcactt gccataggca tgctgattga cgactcggtc gtcgtgcgtg
     1081 aaaacatctt ccgccacctt gagcgcggcg aggatccgtt cacggcagcc cacaagggaa
     1141 ccgccgaaat cgcgctggcg gtcatggcga cgactttcac gatcgtcgcg gtctttgttc
     1201 cgatcgcgtt cacggccggc ctggtcggca ggttcctgaa ggaattcggg atcacggtcg
     1261 cggcggcggt tctggtgtcc ctggtcgttt cgctgacggt ggacccgatg ctttcggccc
     1321 gtcttgtcca ggacatcccg ccggactatc accagaagcg gcgtcagcac aagtttttcg
     1381 gtttcttcat acgtggttac gacgcgcttg attccacata tcgccggatg cttttgtggg
     1441 ccatttccca ccgcaagacc gtcgtgattg cggcaatcgc actgtttgcg ggcagccttg
     1501 ggcttgttca gttgatgggc acccagttca tcccgcgcgg cgaccagtcc gcgttttcga
     1561 tgaaccttga actgccggcc gggaccccgt tgtctgaaat ggatcgcgtg accgccattg
     1621 ttgaaggcat ggttgacgaa ctacctgaag tcacgacgat ttcaacgacc gtcgggccgg
     1681 atgaggccgt aaaccgggcg tacatatatg ttctgaccac tccgcgcaac gaacgcgacc
     1741 gttcggtgac tgacatgatg gaagtcctgc gtccacggct ggcggcgatt ccgggactgg
     1801 tttacagcct gaacgatgcc gacatcttcg gccagtcctt cgcattgatg tcggccgcat
     1861 tcaccatgag gatccgcggg tcggactatc aggaactggg caggatcgcg aatgaagcgt
     1921 ttgagatggt caaatcgacc acaggcatcc gtgacgccgc catttcgttc cgaccgggca
     1981 tgccggaaaa gagctttgtc gtcgatcgca agcggtcggg cgacatgggc gtcgttttcg
     2041 cccaggccgc gatgaccctg cgaatcgggc tgaccggtgc ccccgttgcc aggttccgcg
     2101 acggaagcag ggacatcgat gtgcgcgtcc agcttcgtcc ggaagacagg gccgacgtgg
     2161 aatcagtgct tgacctgaag gttccgacga accgcggcgg aatggtcggg ctgcgtgacg
     2221 tggtcaatgt gaaggacacc gtgcttccgg cgaccataga acgccagaac cgcgaaaggt
     2281 atgtgacgat aacggcgaac cttgccgggc ggccgcttgg aagcgtgatc aaggatctga
     2341 agccgaagct ggacaccata cagaagccgg acggctaccg catcgaattc gccggcgacg
     2401 cggagatgat ggacgaaacg tttgcgaaca tggcccttgc actgattctg ggcgtggtct
     2461 tcatctattt cgtgctcgca tcccagttcg aatcgctggt gcatcccctg acgatcatgc
     2521 tttcgctgcc gctggcgctg gtcggcgcgc tggtgaccct tttccttttc gggatcagcc
     2581 tggacatcat cgcgatgatt gggattgtcc ttctgatggg gcttgtcacc aaaaatgcga
     2641 ttctgctggt cgattacacg aaccagcttc gcgaaaaggg cctgtccatc accgacgccc
     2701 ttctggaagc cgggccgacc aggctgcgtc cgatcctgat gacatcggcc gccatggttc
     2761 tgggcatgct tccgacggcg atcgggaccg gtgcgggggc cgagttccgc gtgcccatgt
     2821 cgatatcggt catcggcggc gtcatctcct cgacttttct tacattgatc gtcgtgccgg
     2881 tcgtctatat ctggatggat cggttcacga tcaagggccg aaggttgcag aagagggccg
     2941 aggcagctac gttcgacgtc gatgagtgat cgtttcgcgg ggggatgaaa tgactgacgg
     3001 acgccttgtg aaggcccggt ttccggcctt cgtgctggtc ttcattgcgg ctgcatccgg
     3061 ctgcggcgat aagccgttcc cctttgacac cggtgggggc gatctgccgg cggatatctc
     3121 gcctgaagtc gcgatatcgg atctggcgat ccgctttacg acaccagccc ctgaatcaag
     3181 gccggtcgtt tccggaattt accaggtggt ttttgactat tcggatcctg aaggcaagcc
     3241 ggccactctg acggccggct tctacaagca cccggaaata cagccatacg tgattcaggc
     3301 ggacgggcct ggcacctatt ccataaatgt cgatacgacc cttgtcgagg acggcaaccg
     3361 gtcggtcgaa atcatcgcgc tttcgagcga cggcaggacc gtttcaaaga acggtaagat
     3421 cctggtcgac aacaaccggc ccgaaatcga gattctggaa cccactccgg cagcgggtgc
     3481 cgggtttgtc gatgaccttt cgttccgggt tcgcgtgacg gattcgggca gcaaggttca
     3541 tttcgtcagg gttaccatcg gccagttcac ctgggagtgg atcccgcagg acggggagcc
     3601 tgcggccgag gttgacacaa ggattgtcga ggacgccgcc aagggaatat acatcgacgt
     3661 cgtggttccg acagaggggt ggcgtggcgc tgaccttgtc atgaaggttg tggcccgtga
     3721 cggggtcgcg gggcgtgacg cggtggtcga gcaccccctg gtgtttgtgg aaaggccggg
     3781 gctggatggc ggcgaaaggg tctttctgcc gcttgtcgaa aacgatgcgc ttgaaagcgt
     3841 ggaaggcatt cgcctggggc ctgcggacga cggtgactgg gcggtgctgg ggagggtccg
     3901 gaagaaggtc ggatacgagc tggtgctttt ccagagatcc acccccggtc acgcggttca
     3961 ggtcgggacg gtgctggccg aagagtgcca gattcacaag atattcgaca tgaacatcga
     4021 cggcatggac gacattctgg cctggtgcgg gttgggcgcc agccgacgta tcgtcgtgtt
     4081 cctgcagggg gcggatcgcg ctttcgtggc ggccgattcg tatccgaccg aacacatggt
     4141 caaggacctg gctgcaggcg atctcaaccg tgatactttc cctgacatcg cgttcgtttc
     4201 cgacgtccag cccctgtgga ccgggatcat gctgtccatc acggaccagt acggagactt
     4261 tctcggatac gggccgccgt cgttctactc gggcgcggtc aggccgacgc acgtcgccat
     4321 cgggcgtttt tccgacgacc agaacaacgc agtcgtggtc ggggcgtccg gcagcgggct
     4381 tgtcacggcc tatccgattg atgaaagcgg cgtgccttcc atgggcgaaa acagcagcct
     4441 ggatcctgac aaccttctta ttgacgacgt ttccgacatg gttgcggtca atttcggcaa
     4501 aaccagccag gacaatgacg cgctggtggt cgtcgattcc cgcaacgtcg aggtgctggg
     4561 cgtggcccgc ctgcgccagg acgcctcgcg cagggtggaa tcggtgcacg agtggcccac
     4621 tggcgccatg cccacggaca tcgcggcagg cgacatcgac gtggacggtg cgccggacat
     4681 cgccgtcctg tgcggcggtt ccaacatggt ggacgtgttc atgggcgtca gggagggagg
     4741 ctactacgag ccggtctccg acgggcgccc gctcctggtc ggttcggcga ccgacgtgac
     4801 gctggccgat atggatttcg acggctacct ggatgtggtg gctttgaccg gcaagcggga
     4861 gatgaaggtg gtctattacc ggaagagcgc ggccggggga tggatggccg gctcccggca
     4921 ggcacttgtg ccggccgccc cgatctcgct ggcggtcggt cacttcgtcg aaccgttgtc
     4981 ggggacgcgc gccggcttcc ttgacgcggc ggttttgtac atgttaccgg acaatcgcgg
     5041 gcctgccctt acggtggcgt cggccgaggc ggtatccggg cagccggtgt cgttcgcggg
     5101 ggagttgctt ctgtcgccca agtaccccac cgggctggtc gcgggcaact tcgacatcga
     5161 cctgaacggt ggcggggcga cttccctgac aaggctggac gacctgatcg taacaagcga
     5221 tgacacgatt tcgccccaga atcctcatac cgcgtgggcc atcctcttcc aggaggcggg
     5281 gcacaacagc gcggtcgcgc agaccgtagg gatcttcgtc gataaatcac cgatgtacgc
     5341 ggcggtc
//
LOCUS       DAOPPA010000201         5338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16873_length_5338_cov_3.612341, whole genome shotgun sequence.
ACCESSION   DAOPPA010000201 DAOPPA010000000
VERSION     DAOPPA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16873_length_5338_cov_3.612341"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKH54_07220"
     CDS             <1..542
                     /locus_tag="PKH54_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC99718.1"
                     /translation="PDGDMGYPIIEIYLTAGELKTALEIIVGVAVSIADSFFLQISGL
                     KFEYKPSADWFFMVTKVYMADGNGGWMAEPLDTSEANTKLYRVATNLYIGEMLGVVKE
                     YLGGMVSLEPKNAEGVPYEHISDAIMDIDPDTDGIQELKLWRAFYQYLADMAVPDGAT
                     LPAVPAEYGTEMGRMKAVP"
     gene            556..2733
                     /locus_tag="PKH54_07225"
     CDS             556..2733
                     /locus_tag="PKH54_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99719.1"
                     /translation="MEDLLFTLQSVISATVLNGLVAGGAWRLAAAVAPARAGRPGLSA
                     LDRAIVALAVMTAAVLAATLSLGALGVLSRWSVTVFAAVFAAAAFLSTRRAPREPFVG
                     KLVLAALSGLRQDRLLVIAVLPGVVALTLLMAWAAVRPAYGFDPLNYHLPLMASVLRS
                     GSLDPVHFPPYFETFPYFTMNGDLSALWAMLSTGDASMLPFSNVVHFLLLGLVLYAFV
                     RESLSSRAPAAALVMALLTVPAFFVLLADAYVEIQLWAFMFAALRLIVLSGRNRAHAG
                     LFVLAGIMCGAMVGTKLTGIPMAAVLLAAWLFCTESAGARASAWRVAAFLGMTVVFGG
                     YFYVRNMVTVGSPVYPFPMSIGGLQLFDGDAEHAARVGGTTIRRFLVPLVFSGELFKA
                     IVGTRTPPSSSWGLGVTGPFFILAAFASFIHVFRAARSRARECRSAWLGPVFLGASLL
                     AIVVYLHLPWCAPYLYGNVRFVYPAVPFAALAFVSIPGVAAVRRWFVVGVCLVLQLLS
                     FLFATVPTTTGAVVTALVVVVAVGIVSWKSNRPWFVRMAPWLPAVVAFVAVLAALMLR
                     IPSVHAYGQPGIVSGPALWASERAECVAELDRVLDRGPFAVTMARGAPHAWFLFPLLG
                     ERFQRQPVYVDISRQGAAGALKSADSLPGFPGADRDFWISGMRASGARVLVVLNDPED
                     VEIGRTPVELEWVGQMPETFFTVFSGKYCSIFSFEREDAAAGE"
     gene            2739..3500
                     /locus_tag="PKH54_07230"
     CDS             2739..3500
                     /locus_tag="PKH54_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOC99720.1"
                     /translation="MNVFAVGVLASLLAGLATAVGALPAIFFRNVGKATQNILLGFGA
                     GVMLAATSFSLIIPALDHAGEGVGAALKVAAGIVFGGFFLWLADRILPHEHFTNGPEG
                     SDSKRLRRIWLFVIAIVIHNFPEGLAVGVGFGAGSFSDGLSLAIGIGLQNMPEGLVVA
                     LALLGEKYGLGKALGVALLSGLVEPIGGAFGAGLVSVFGQVLPLALAFAAGAMLYVVS
                     DEIIPEIHSEQKTAGATWGLVIGFIIMMILDVSLG"
     gene            3555..>5338
                     /locus_tag="PKH54_07235"
     CDS             3555..>5338
                     /locus_tag="PKH54_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99721.1"
                     /translation="MKKVPFVPAFSGGHRWAFARIGGFNQVMLTTGDDLRHLGELDEK
                     LFSALSCPTKGLEYDGATLSLLDTDADGRIRVAEIKAAVAWVCARLKDPAVIMSGSDT
                     LELASIDDSRPEGSALLASAKHILSCIGKGDADRICLADVTNRAAIFAESEFNGDGVL
                     PPAAVKDAELRAVVSDILKAVGGSSDRSGAAGIDADRLQDFMAQAGAWVAWKTAPDAD
                     KAILPLGDATGAAFDAFVAVRDKVEDYFARCRLAAFDKRSVAALNREEKDYLALAARD
                     MKITDAEIQALPLAAVDAGRPLQLVNGVNPAWIDRIGAFREKVASALNGAQLSSISWE
                     QWQAIVAKMAPFQAWVEAKPAGSAVAALEEIGVERVRAIIGGEYGQKITDLMAADKAV
                     EGEAAAIDDLRKLLYFHKNLFKLLKNFVSFAEFYQRSDKSVFQVGTLFIDNRSCDLCI
                     RVNDMGKHALMAGSSRMFLLYCDCVRPSGEKIQIAAAVTDGDSDDLIVGRNGIFYDRQ
                     GRDWDATITKIIENPIGVRQAFWSPYKRLMRFVEEQVAKRAAAGDAASEDKLKGAALA
                     APGVAKDAADGKAVPPAKSKFDVGVVAAL"
BASE COUNT          816 a         1566 c         1758 g         1198 t
ORIGIN      
        1 ggccggacgg cgacatggga tacccgatca tcgagatcta cctgaccgcc ggtgaactga
       61 agaccgcgct ggaaatcatc gtcggcgtcg ccgtttcaat cgccgattcg ttcttcctgc
      121 agataagcgg gctgaagttc gaatacaagc cgtcggccga ctggttcttc atggtcacca
      181 aggtttatat ggccgatgga aacggtggct ggatggccga gccgctggac acgtccgagg
      241 cgaacacgaa gctgtaccgc gtcgcgacga acctttacat cggcgagatg cttggcgtcg
      301 tcaaggagta ccttggcggc atggtcagtc tggaaccgaa gaacgcggaa ggcgtcccat
      361 acgaacacat cagcgacgcc atcatggaca tcgatccgga cacggatgga atccaggaac
      421 tgaagctgtg gcgcgcgttc taccagtacc tggccgacat ggccgttccg gacggtgcca
      481 ctcttcccgc cgttcccgcg gaatacggca ccgagatggg gcgcatgaag gccgttccgt
      541 gataatcttc cgtcgatgga agacctgctt ttcacacttc agtcggtgat atcggcgacc
      601 gtgcttaacg gactggtcgc gggcggtgca tggcgccttg ccgcggcggt cgctccggca
      661 agggcggggc gtcccgggtt gtccgccctg gatcgcgcca tcgtggccct ggccgtcatg
      721 acggcggcgg tgcttgccgc cacgctctcg cttggcgccc tcggcgttct ttcccgctgg
      781 tcggtcactg tcttcgcggc cgtcttcgcc gccgcggcgt tcctgtcaac ccgaagggct
      841 ccgcgcgagc cgttcgtcgg gaagcttgtt cttgcggccc tgtccggtct gcgccaggac
      901 aggctgcttg tcattgccgt gcttcccgga gtcgtggccc tgacgctgct gatggcctgg
      961 gccgccgtcc gtccggcata cggctttgat ccgttgaatt accatctgcc ccttatggca
     1021 tcggtgttgc ggtccgggtc gctggacccg gttcacttcc cgccgtattt cgagacgttt
     1081 ccatacttca ccatgaacgg cgacctttcc gcgttgtggg cgatgctttc aaccggcgac
     1141 gcctcgatgt tgccgttttc caacgtcgtc cacttccttc tgcttggact ggttctgtac
     1201 gcgttcgtgc gtgaatccct ttcgtccagg gccccggccg cggcacttgt catggcgttg
     1261 cttacggtgc cggcattctt cgtcctgctt gccgacgcat acgttgaaat ccagttgtgg
     1321 gcgttcatgt tcgcggccct gcggctgatt gtcctgtccg gacggaatcg ggcccatgca
     1381 gggctttttg tgctggccgg catcatgtgc ggcgccatgg tcggcacgaa gctgaccggg
     1441 attccgatgg ccgccgtgct tctggccgcc tggcttttct gtaccgagtc ggctggcgcg
     1501 cgcgcatcgg cctggcgcgt cgccgccttc cttggcatga cggtcgtctt cggcgggtat
     1561 ttctacgtcc ggaacatggt gacggtggga tcgccggtct atccgttccc gatgtcgatc
     1621 ggcggcctgc aactgtttga cggcgacgcg gagcatgcgg ccagggtcgg gggaaccacg
     1681 atcaggcgct tcctggtgcc ccttgtcttt tcgggcgagc ttttcaaggc cattgtcggc
     1741 acaagaacgc cgccgagttc gtcgtggggg ctcggtgtca ccggtccgtt cttcatcctg
     1801 gccgcattcg cgtccttcat tcacgtcttc cgggcggccc gcagccgggc cagggaatgt
     1861 cgatccgcgt ggcttgggcc ggtcttcctg ggcgcgtcgc tgcttgcaat cgtcgtctat
     1921 ctgcaccttc cctggtgtgc gccgtatctg tacgggaacg ttcgcttcgt ctatcccgcg
     1981 gtgcctttcg cggcgctggc gtttgtttca attccggggg tggccgcggt caggcgatgg
     2041 tttgtcgttg gcgtctgcct ggttcttcag ttgctatcct tccttttcgc gaccgttccg
     2101 acaacgaccg gtgccgttgt cacggcactc gtcgtggttg tcgcggtcgg catcgtttca
     2161 tggaagtcga acaggccatg gttcgtccga atggcgccct ggctgccggc ggtggtcgca
     2221 ttcgtggcgg ttcttgccgc attgatgctt cggattcctt ccgtgcatgc ttacgggcag
     2281 cccggcattg tatcggggcc ggccctgtgg gcgtccgaac gtgccgaatg cgttgccgaa
     2341 cttgaccggg tgctcgaccg cggacctttt gccgttacta tggcccgggg cgccccgcac
     2401 gcatggttcc tgtttcccct gcttggcgaa aggttccagc ggcagcccgt atatgtcgat
     2461 atcagccgtc agggcgccgc gggggccttg aagtccgccg actcgctgcc cgggtttccc
     2521 ggcgccgatc gtgatttctg gatttcgggg atgcgggctt ccggcgcgcg tgtcctggtc
     2581 gttctcaatg atccggagga cgtcgagatc gggcgcacgc cggttgaact ggaatgggtc
     2641 gggcagatgc ctgaaacgtt tttcaccgtc ttcagtggaa agtactgcag catcttttcc
     2701 tttgaacggg aagatgcggc cgcgggggaa tgaccgtcat gaacgtattc gccgtcgggg
     2761 tgcttgccag cctgctcgcc ggacttgcca ccgcggtggg ggcgcttccg gccatctttt
     2821 tcaggaatgt cggcaaggcc acccagaata tcctgcttgg gttcggcgct ggcgtcatgc
     2881 tggcggccac ttcgttttcc ctgatcatcc cggcgcttga ccatgccggg gaaggggtcg
     2941 gcgcggcttt gaaggtggcc gcgggaatcg tatttggcgg gttcttcctg tggctcgctg
     3001 accgcatcct tccgcacgag catttcacca atggcccgga aggttccgat tcaaaaaggc
     3061 ttcgccgcat ctggctgttc gtgatcgcga tcgtaatcca caatttcccc gagggccttg
     3121 ccgtcggggt cggtttcggg gcgggcagct tcagcgacgg tctttcgctg gcaatcggaa
     3181 tcggcttgca gaacatgccg gaaggcctgg tcgtcgcgct ggcccttctg ggcgagaagt
     3241 acggccttgg aaaggcgctc ggggtggccc tgctttccgg gctggtcgag ccgattggcg
     3301 gggcgttcgg tgccgggctt gtgtcggtgt tcggccaggt gcttccgctg gcgctcgcct
     3361 ttgctgcggg cgccatgctt tacgtggtca gcgatgaaat catcccggaa attcacagcg
     3421 agcagaagac ggccggggcg acctggggac tggttatcgg cttcataata atgatgattc
     3481 ttgatgtaag ccttggatga ttgccgaatt gggctagaat caatcggtct tatcaccttc
     3541 gggaagaggt tcgtatgaaa aaggttccgt tcgttccggc gttttccggc ggtcatcggt
     3601 gggccttcgc aaggattggc gggtttaacc aggtcatgct gaccactggc gacgacctgc
     3661 gtcacctggg ggaactggac gagaagcttt tttccgcgct ttcctgcccg acgaagggtc
     3721 ttgagtacga tggggctacc ctttccctgc tggataccga tgccgacggc aggatccgcg
     3781 tggccgaaat taaggctgcc gtcgcctggg tatgtgcgcg tttgaaggat cccgccgtca
     3841 tcatgtccgg aagcgacacg ttggaactgg cgtcgattga cgattccagg ccggaagggt
     3901 ccgctttgct tgcatccgcg aagcacatac tttcgtgcat cggaaagggc gatgcggatc
     3961 ggatctgcct tgcggacgtg accaatcgcg ccgcgatctt cgccgagtcc gaattcaacg
     4021 gcgacggcgt cctgcccccc gcggcggtca aggatgccga actgcgggcg gtggtgtcgg
     4081 acatcctcaa ggctgtcggt ggctcgtccg atcgcagcgg tgctgccggt atcgatgccg
     4141 accgtcttca ggacttcatg gcccaggccg gcgcgtgggt tgcatggaag acggcaccgg
     4201 atgccgacaa ggcgatcctg ccgctggggg atgcgaccgg cgcggcgttt gatgccttcg
     4261 tggctgttcg ggacaaggtc gaggattact tcgccaggtg ccggctggcg gctttcgaca
     4321 agcgttcggt ggccgccttg aaccgcgagg aaaaggatta tctcgcgctt gcggcgcgcg
     4381 acatgaagat taccgatgcc gagatccagg cgttgcccct ggcggcagtc gatgccgggc
     4441 ggccgcttca gcttgtcaac ggcgtcaacc cggcctggat tgaccgcatt ggcgcgttcc
     4501 gcgagaaggt tgcgtcggcc ctgaatggcg cgcagctgtc ctcgatttcc tgggagcagt
     4561 ggcaggcgat tgtcgcgaag atggccccct tccaggcgtg ggttgaggca aagccggccg
     4621 ggtcggctgt cgcggcgctg gaggaaatcg gggtcgaacg tgtccgcgcg atcattggcg
     4681 gcgagtatgg ccagaagatc accgacctta tggcggccga caaggcggtc gaaggtgaag
     4741 ccgcggccat cgacgatctt cggaagttgt tgtatttcca caagaatctt ttcaagctgt
     4801 tgaagaactt cgtgtcgttc gccgagtttt atcagcgaag cgacaaatcc gttttccagg
     4861 tcggaacgct cttcatcgac aaccgcagtt gcgatttgtg catacgtgtc aacgacatgg
     4921 gcaagcacgc gttgatggcc ggttcaagcc ggatgttcct gctgtattgc gactgcgtca
     4981 ggccgtcggg tgaaaagatc cagattgcgg cggcagtcac cgatggcgac agcgacgacc
     5041 tgatcgtcgg aaggaacggg atattctatg accgccaggg gcgcgactgg gatgcgacga
     5101 taacgaagat aatcgaaaac ccgattggcg tcaggcaggc attctggtcg ccgtacaagc
     5161 gtctgatgcg ctttgtcgag gaacaggtcg caaagagggc cgcggctggc gatgctgcgt
     5221 cggaagacaa gctgaagggc gccgcgctcg cggcccccgg cgtcgcgaag gatgcggccg
     5281 acgggaaggc cgttccaccc gccaagtcga agttcgatgt cggcgtggtc gccgccct
//
LOCUS       DAOPPA010000202         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16893_length_5332_cov_3.695092, whole genome shotgun sequence.
ACCESSION   DAOPPA010000202 DAOPPA010000000
VERSION     DAOPPA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16893_length_5332_cov_3.695092"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1212)
                     /locus_tag="PKH54_07240"
     CDS             complement(88..1212)
                     /locus_tag="PKH54_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99722.1"
                     /translation="MMQNLTNFDLVVFDMEDALVDRSDALGSAVSRAVDAYLTMIVGL
                     RPEGGPVYSIPQVSEFVSRQQFEEPPDVFHALLAFAAHVLPIDLREDDFRDYDGRDLL
                     DAVKNSGKVNISLGELSKLCNVQEFSKILRAKGGAIKGLNRQRSLKNQWLVLAEGHIM
                     MDNLVKRIFAEVYLENELFLKEYGVERRYITDKACIRREKGYIDPQDFEMIRRQCPVA
                     VVTSRDQSEAQWVLNNIGIARSVDVVVSADAMGMGMADPEEVVWIRSLGVGGAEAADY
                     STRVADAIERTRAQEALETVLRIGWVGDCSVEGRNLASLKERYGMTLIGIAFGDDPKI
                     SAALKDKGADIVVSDVDSMVKAITERSGRSRRPMRQQYPD"
     gene            complement(1247..2920)
                     /locus_tag="PKH54_07245"
     CDS             complement(1247..2920)
                     /locus_tag="PKH54_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOC99723.1"
                     /translation="MAADLRTLKIVALTMLLAVSLTACSERRSRDRETVPWEDVVKGL
                     SEPPGSAGYSIAMLSLTRSWKQHGQEGAVLMIREGLELIGLSANGAIHGASAMLDADV
                     VAGEKNMIGFMLASLSGAAEMVSDEWFRYHVHLTAALGVGGGKQLQDLLDMADAGTHE
                     LAESVRLCLALPLSNALIAAASSLPTERGRVLLERLPGWPSPPTTDLMQTLFPANLAR
                     ISRWISEGRKHGGLHELAWTNVASRMEKGLARRLYDLPLVVDAEPRKGTPVSAIGAGY
                     TPLEVARIMKDGVAVYLRPVLQWDKAFMTVVSGDATWRPGKPDDAAGDVSVATLAERL
                     KALHELTGKLEKGVYPTLVGTGMGDRAGRSILIAVDSDATAGTMVEALRALAAAGYSD
                     FRIATPGAPGTTNPIMVAGSTGMLSTDRTVRVFLTPAGASIFPNPAAGLASDNLSAGV
                     SPIGPGSVGYFAAWDAERGFSSSLAMAFQDMGAVGAGHVEVAEIFLTAGDPPAMMLVD
                     VASELQAAPGIQFSRIEPNFPGMICPQGRSCPGVVPVFGGTARVPAGVK"
     gene            complement(3055..3603)
                     /gene="frr"
                     /locus_tag="PKH54_07250"
     CDS             complement(3055..3603)
                     /gene="frr"
                     /locus_tag="PKH54_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018660095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOC99724.1"
                     /translation="MTDQVFDNLDEAFLKAIANYERDLSRVRTGRANLALLEGLKVEY
                     YGVPTPLSQVAALSVPDPRLIVVKPWDKKLISYIEKAVINADLGLSPNNDGDVVRLPI
                     PPLTTERRKDLVKQVKKMCEETKIAVRNARRDGRAELDKIENLPEDEKKRAQERIDKE
                     TDKQIKSVDDIATRKEKEIMDF"
     gene            complement(3606..4355)
                     /gene="pyrH"
                     /locus_tag="PKH54_07255"
     CDS             complement(3606..4355)
                     /gene="pyrH"
                     /locus_tag="PKH54_07255"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOC99725.1"
                     /translation="MTYENAVGSNCTCRFRRVLLKLSGEVLQGPGSGGIDPATVDRIA
                     GEVARVRQMGVQVGMVIGGGNFFRGISTLGRNFDRPTADQVGMLATVMNALIFSDALK
                     RQGCPSVVMTAIEMPRVAETFVRARADSLLSDGTVVIFCAGTGHPYFSTDTGAALRAL
                     ETGCDVMMKATKVDGVYSADPARNVDAVRYDKLSFRQVLDDGLGVIDMAAATLCAENG
                     LPLLVFSILEPGNLERAVIDDTVGTIVSHGG"
     gene            complement(4431..5030)
                     /gene="tsf"
                     /locus_tag="PKH54_07260"
     CDS             complement(4431..5030)
                     /gene="tsf"
                     /locus_tag="PKH54_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOC99726.1"
                     /translation="MAIDSKLVKELREMTGAGILDCKNALESSNGDMNGAIEYLKKRN
                     QAAVVKKMSREAKDGKIASYIHGDGRIGVLVEINSETDFVARSEGFQDFVKEVMLQIT
                     SMNPKYLKPEDIPEADMAAQKDIFMGQMEDVKKPEEVKAKIIEGKMAKWFTEVCLLEQ
                     PYIRDDSKKVKDLLTEVVGRCGENIRVRRFMRFELGEGL"
     gene            complement(5089..>5332)
                     /locus_tag="PKH54_07265"
     CDS             complement(5089..>5332)
                     /locus_tag="PKH54_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOC99727.1"
                     /translation="DTNCDPDNVSYVIPGNDDAIRSIKLIMKSFADSCIEGAQHRKTG
                     GQEWGSVPSVDEKGPEIVFRGAHKAEEPEAAPTADE"
BASE COUNT         1077 a         1727 c         1447 g         1081 t
ORIGIN      
        1 caagctgtgc ttcattcccg aatcgatgtc gattacggac tggaccgaga tcaaccccgg
       61 ggactacggc gtgcgctgaa tcgatgacta atcgggatac tgctgacgca tcgggcggcg
      121 tgaacggccg gaacgctcgg taatggcctt caccattgaa tcgacgtccg aaacgacgat
      181 gtccgcaccc ttgtccttca aggccgcgga aatcttcggg tcatcgccaa acgcaatccc
      241 gataagcgtc atcccgtatc gttccttcag ggaagccagg ttcctgcctt cgacagagca
      301 atccccgacc cacccgattc gcagcaccgt ttccagcgcc tcctgggccc tggtccgctc
      361 gatcgcgtcc gcgaccctgg tgctgtaatc ggccgcctcg gcgccaccga ccccgaggct
      421 gcggatccag acgacctcct ccggatcggc cattcccatg cccatcgcgt cggcgcttac
      481 caccacatcg accgaccttg cgatcccgat gttgttcaga acccactggg cctcggactg
      541 gtcacgcgaa gtgacgaccg ccaccggaca ctgacggcgg atcatctcga agtcctgggg
      601 atcgatgtat cccttttccc tgcggatgca agccttgtcg gtgatgtacc gcctttcgac
      661 gccgtactcc ttcaggaaca gttcgttttc cagatagact tcggcgaaga tgcgcttgac
      721 caggttgtcc atcatgatgt ggccttcggc caggaccagc cactggttct tcaatgaacg
      781 ctgcctgttc aagcccttta tggcaccgcc cttcgcgcgg agaattttcg aaaactcctg
      841 gacgttgcag agcttcgaaa gctcgccaag gctgatgttg actttgccgg aattcttgac
      901 ggcatccagc agatcgcgac cgtcgtaatc gcggaagtca tcctcgcgaa gatcgatggg
      961 aagcacgtgg gcggcaaaag ccagcagcgc gtggaatacg tccggcggtt cctcgaactg
     1021 ctgacgcgaa acgaattcgg acacctgtgg aatggaatag accgggccgc cttcggggcg
     1081 cagaccgaca atcatcgtca ggtaagcatc cacggccctg ctgactgccg aaccgagagc
     1141 atcgctccga tcgaccagcg cgtcctccat gtcgaacacg accaggtcaa aattcgtcaa
     1201 attctgcatc atcaccttcc cgcgccccgg ccggacgcga caaattctac ttcacgcccg
     1261 ccggcacccg ggcagtccca ccgaaaaccg ggaccactcc cggacaggac cttccctggg
     1321 gacatatcat gccgggaaag ttcggttcga tacgcgaaaa ctggattcca ggcgcagcct
     1381 gcagttcgga cgccacatcg acaagcatca tcgcgggcgg gtcgccggcc gtcaggaata
     1441 tttcggcgac ttccacgtga ccggccccga ccgcccccat gtcctggaat gccatggcaa
     1501 ggctggacga aaatcccctt tcggcgtccc atgcggcgaa atatccgacc gagccaggac
     1561 cgatgggcga taccccggca gaaagattgt ccgaggcaag tccggccgcg ggattcggga
     1621 aaatggaagc cccggcggga gtcaggaaca ccctgaccgt gcgatccgtg gacagcatcc
     1681 cggtggaacc ggcaaccatg atcgggttcg tggttcccgg ggcgcctggc gttgcgatcc
     1741 tgaaatcgga atacccggcc gcggcgagtg cccgaagcgc ctcaaccatc gttccggctg
     1801 ttgcatcgga atcgaccgca atcagtatcg aacggccggc acgatcgccc atgccagttc
     1861 ccaccagcgt cggatagaca cccttttcga gcttccctgt aagctcatgc aaagccttca
     1921 ggcgttcagc caacgtcgcc accgatacgt cgcccgcggc atcatccggc ttgccaggcc
     1981 gccatgtcgc atcacccgag acaaccgtca tgaaagcctt gtcccactga aggacggggc
     2041 gcaggtaaac agccacgcca tccttcatga tcctggcgac ttcaagcggg gtgtatccgg
     2101 ccccgattgc ggaaaccggc gtgcccttgc gcggctcggc atcgacgaca aggggcaggt
     2161 cataaagtcg gcgtgcaaga cccttttcca tcctggacgc gacgttggtc caggccagtt
     2221 catgaagccc gccgtgcttg cgtccttcgg aaatccagcg cgaaatcctg gccaggttgg
     2281 ccgggaacag cgtctgcatc aggtcggtgg tgggcggact gggccacccg ggaagccttt
     2341 ccagcaggac tcgcccacgc tcggtcggca gcgacgacgc ggcggcgata agcgcgttcg
     2401 aaagcggaag ggccaggcag agcctgacgc tctcggcaag ttcgtgcgtc ccggcatccg
     2461 ccatgtcgag cagatcctgc aactgcttgc ccccacccac gccaagggcg gccgtcagat
     2521 gcacgtgata cctgaaccac tcgtcggaaa ccatttcagc ggcgcccgac aggctggcca
     2581 gcatgaaccc gatcatgttc ttttcgccgg ccacgacgtc ggcatcaagc atggccgatg
     2641 ccccatgtat cgcgccgttg gcggaaagac caataagctc aagaccttcc ctgatcatca
     2701 ggacggcacc ttcctggccg tgctgcttcc atgacctggt cagcgaaagc atcgcgattg
     2761 aatatccggc agaaccgggc ggctcggaaa ggcctttgac cacgtcttcc cacggaacgg
     2821 tctcgcgatc cctcgaacgc cgttcggaac acgccgtaag ggacacggcc agcagcatcg
     2881 tcaatgcaac aatcttcagg gttcttaaat ctgccgccac tgtgaacctc gccaaaaagt
     2941 ccgatccgcg ctgagtatat ccaatgattg gatccgtcgt ccatgccgac cggtcacgaa
     3001 cctggatcac ttctgcaact gtcatgcatc aaaaaacggc acaaagcggg gaaatcagaa
     3061 atccatgatt tccttttcct tgcgggtggc gatgtcgtca acggacttga tctgcttgtc
     3121 ggtttccttg tcgattcgct cctgggcccg cttcttctcg tcttccggca gattctcaat
     3181 cttgtcgagt tcggcacggc catcccttcg ggcattcctg accgcgatct tggtttcctc
     3241 gcacatcttc ttgacctgct tgaccagatc cttgcggcgc tcggtcgtca gcggcggaat
     3301 cggcaggcgc acgacatcgc cgtcattgtt cggcgaaagc cccagatccg cgttgataac
     3361 ggccttctcg atgtacgaaa tcaacttctt gtcccacggc ttgaccacga tcagcctggg
     3421 atccggcacc gacagcgccg cgacctgcga cagcggcgtc ggcacgccgt agtactcgac
     3481 cttcaggcct tcaagcaacg caagattcgc gcgcccggtc ctgaccctgg aaagatccct
     3541 ttcgtaattg gcgatcgcct tcaggaaagc ctcgtctaga ttgtcaaaaa cctggtcagt
     3601 catcatcacc ctccatgcga aacaatcgtt ccgaccgtat cgtcaatcac ggcccgctca
     3661 agattgccgg gctcgagtat ggaaaacacc agcagcggca ggccgttttc ggcacacagg
     3721 gtggccgcgg ccatgtctat cactcccaga ccgtcgtcca gaacctggcg aaaagataat
     3781 ttatcatatc tgaccgcgtc aacatttcgc gccggatcgg ccgaatatac tccatcgacc
     3841 ttcgttgcct tcatcatcac gtcgcacccc gtctccaggg cccgcagggc ggcgccagtg
     3901 tcggtcgaaa agtagggatg tccagttccg gcgcagaaga tgacgaccgt gccgtcggaa
     3961 agaaggctat cagcccttgc ccttacaaag gtctcggcga ccctcggcat ttcgatcgcg
     4021 gtcatcacga cagacggaca tccctggcgc ttcagggcgt ccgagaagat aagcgcgttc
     4081 atcaccgtcg ccagcatgcc gacctgatcg gcggtcggcc gatcgaagtt gcgcccgagc
     4141 gtcgaaattc ccctgaagaa attcccgccc ccaatgacca tgccgacctg gacgcccatc
     4201 tggcgtaccc tggccacctc acctgcaatg cggtcaacgg tcgccggatc gatgcccccg
     4261 gaacccggcc cctgcagcac ctcgccggaa agcttcaaca gaacccgtct gaacctgcaa
     4321 gtgcagtttg aaccaacggc attttcgtac gtcactggaa cctccggcat tttcttgaaa
     4381 cgcggattct tatgctggcg gggattataa ccgggaaaga tcgaaatggc ctagagtcct
     4441 tcacccagtt cgaaacgcat gaaccggcgg acgcggatgt tttcgccaca gcggccgacg
     4501 acttcggtca acagatcctt gaccttcttc gaatcgtcgc ggatataggg ctgctcgagc
     4561 aggcagactt ccgtgaacca cttggccatc tttccttcga tgatcttcgc cttcacttcc
     4621 tcgggcttct tcacgtcttc catctggccc atgaagatgt ccttctgggc ggccatgtcg
     4681 gcctcgggaa tgtcttccgg cttcagatac ttcgggttca tggacgtgat ctgcagcatc
     4741 acttccttca cgaaatcctg gaaaccttcc gatcttgcga cgaagtcggt ttcactgttg
     4801 atttccacca gaacgccgat gcggccgtcg ccgtggatat agctggcgat cttgccgtcc
     4861 ttcgcctcgc ggctcatctt cttcacgacc gccgcctggt tgcgcttctt aaggtattcg
     4921 atggcgccgt tcatgtcgcc attggaagat tcaagcgcgt tcttgcaatc aagaatgccg
     4981 gcgccggtca tttcacggag ttccttcaca agcttgctgt cgatagccat aagaacttct
     5041 ccttgtattt tggggcccaa aaaggccatt catcggaacg tgactggatc actcgtccgc
     5101 ggtcggggcg gcttccggct cctcggcctt gtgcgcgccg cggaagacga tttccggacc
     5161 cttttcatcg acggaaggaa ccgaacccca ctcctggccg ccggtcttgc gatgctgcgc
     5221 gccttcgatg caggaatcgg cgaagctctt catgatgagc ttgatcgacc ttatggcgtc
     5281 gtcgttgccc gggatgacgt acgaaacgtt gtccgggtcg cagttcgtgt ca
//
LOCUS       DAOPPA010000203         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_16929_length_5322_cov_3.912474, whole genome shotgun sequence.
ACCESSION   DAOPPA010000203 DAOPPA010000000
VERSION     DAOPPA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_5322_cov_3.912474"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKH54_07270"
     CDS             complement(<1..501)
                     /locus_tag="PKH54_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99728.1"
                     /translation="MSRRKSDKVDDLAIGLMDRHSHVPTGEEQLERYATMYLGRPVGY
                     EHAVEVVEEAAPAEAFEPQFEQPIDIPVAAEMDAGVAASYEDVDNDYDRPGATVVFDA
                     MDVPAADSDWDSNPANQVEPAPEPADASMEATMTFEAAELADAPQVEFDPEGENSTVM
                     LDALDDQ"
     gene            complement(536..3157)
                     /gene="alaS"
                     /locus_tag="PKH54_07275"
     CDS             complement(536..3157)
                     /gene="alaS"
                     /locus_tag="PKH54_07275"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOC99729.1"
                     /translation="MKSAEIRQKFLQFFEERGHRIVASHGLIPPADPTLLFVNAGMVQ
                     FKDVFAGYRDPGYRRATTTQKCLRVSGKHNDLDEVGRTPRHHTFFEMLGNFSFGDYFK
                     RDAIVYAWDLLTKVYGLDPADLWVTVHPDDDEARQLWITQAGVAPDRIIADPDDFWSM
                     GDTGPCGPCSEIYVDRGPSYSGTSMHDQGHRFMEIWNLVFMQFNRDEAGVLHPLPAPS
                     IDTGMGLERIASVLQGVRTNYDTDLLMPLIERAAAAAGIRYGQDPDNDTALRVIADHA
                     RATAFLISDGVFPENEGRGYVLRRLMRRALRFGHKLGFRGPFFTDIVLFVSTVMGAAW
                     PELQRDADVIARIAGQEEERFLKTLSSGMELLDAAVRRAREEGRAGIDGQTVFTLYDT
                     HGFPVDLTEVVAEENGMGIDRDGFNALMEEQRARGRASWKTHDASWGGIAEHCRVNAI
                     KSEFVGYDYDEAAGNPVSSVVAAIFVDGSSVESASAGADAVVVCRRTPFYAESGGQVG
                     DTGTISGEGYLFRVSDTRKQGDDCFMHVGKVESGVLKVGHEAGLVVNRARLDTRRNHS
                     TTHLLHRALRQVLGTHVRQRGSQVSPERLRFDFSHMAPMTRQEIEQVEKLVQGWIFDD
                     LPIVTREMPIEAAIAEGALHFFEDKYGESVRMVRMGDSVELCGGTHAARTSAIGLFKI
                     VSEGGISAGVRRIEALTGSAAVAWFRERDRMLAEASEVLRCDGSQVAEKASRLVDTEK
                     SLRKELADLKVKVAAAGSSGGDAGRIVELGGIKVMVVPADGMDRKGLRELADVMRDRV
                     GSGMVLITVAEGAKMSVVLAVSADLQKERPAGTTLNQVLAGIGGRGGGSPSLAQGSVD
                     TPADRGVILDALAKALQ"
     gene            complement(3194..4258)
                     /gene="recA"
                     /locus_tag="PKH54_07280"
     CDS             complement(3194..4258)
                     /gene="recA"
                     /locus_tag="PKH54_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOC99730.1"
                     /translation="MAKEPRDTKLKVGETAEQRERNIEVAVATIERQFGKGSIMRMAG
                     DASTVGRVPVISSGSLGLNIALGIGGYPKGRIVEIYGQESSGKTTLTLLAIAECQRAG
                     GLAVFIDAEHALDVNYARKLGISVEDLLLAQPDTGEQALEIAETLVRTNAVDLVVIDS
                     VAALVPKAELEGEMGDSHVGLQARLMSQALRKLAGVLSKSNTCMIFINQIRMKIGVMY
                     GNPETTTGGNALKFYASIRLDVRRGQAVKEADGQTTTGSRVKVKVVKNKMAPPFREAE
                     FDLMYGEGVNRLGELVDLAGQIGVLEKSGSWYSYAGKRLGQGREQAMAFLKDNADDLA
                     AVTRDVYSKFGITVDDTPAA"
     gene            complement(4294..4950)
                     /locus_tag="PKH54_07285"
     CDS             complement(4294..4950)
                     /locus_tag="PKH54_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99731.1"
                     /translation="MTVESDNRKAEQVRKAAIVLEPSIQFANLLLDVTRDTRKRLGRG
                     AGAVWMPPAACAITILTAELPPRALDEVIGMGMSSVASGFRDVQICIDPPSLERLADG
                     SMLIISRAWSTDSVIGVISGALARELERVGVESVDQVSGGLKLVYGWMPGASEIASIP
                     GAASFEYPSRIAISGLVGGILEPVGTTPMTLCRRVRTAPLAIAAGSRHVRDSGGVQEV
                     "
BASE COUNT         1042 a         1779 c         1488 g         1013 t
ORIGIN      
        1 ctgatcgtca agggcgtcaa gcatgactgt gctgttttcg ccctcggggt caaactcgac
       61 ctggggcgcg tcggcaagtt ccgcggcctc gaaggtcatc gtggcctcca tcgaggcgtc
      121 cgccggctct ggtgccggtt caacctggtt cgctggattc gaatcccagt cggaatccgc
      181 cgccgggacg tccatcgcat cgaagaccac ggtcgcgcca ggcctgtcgt aatcgttgtc
      241 aacatcttca taagacgcgg ccacgccggc atccatttcc gcggcgaccg gaatatcgat
      301 cggctgctcg aactgcggct cgaaggcttc ggccggggcc gcttcctcaa caacctcgac
      361 ggcatgctca tagccgacgg gacggccaag atacatcgtc gcgtatcgct caagctgctc
      421 ctcgcctgtc ggaacatgcg aatgcctgtc catcaggcca atcgccagat catcaacctt
      481 gtcggacttc ctgcgggaca tctgaagcct cctgcggcaa tcaaacggtt aagtattatt
      541 gaagggcttt ggcgagggcg tcaagtatca cgccccgatc cgccggagta tcgaccgaac
      601 cctgtgcaag cgaaggggag ccaccacccc ttccgccgat tccggcaagc acctggttca
      661 gcgttgttcc ggccggcctt tccttctgca aatcggccga taccgcaaga accaccgaca
      721 tcttcgcacc ttccgccacg gtgatcagaa ccatgccgga accgaccctg tcgcgcatca
      781 cgtccgcaag ttcgcgcaac cctttccgat ccatgccatc ggcgggcacg accatcacct
      841 tgatgccgcc aagttcgaca atcctgccgg catcgccacc ggacgagccc gcggcggcga
      901 ctttgacctt caggtctgcc agttccttgc gaagcgactt ctcggtatcg acaagacggg
      961 aagccttttc ggcaacctgt gatccatcgc agcgaaggac ttcggacgcc tcggcaagca
     1021 tcctgtcccg ctccctgaac catgcgaccg ccgccgatcc ggtcagtgcc tcgatgcgac
     1081 ggactccagc cgaaattcct ccctcggaaa caatcttgaa cagaccgatg gcggacgtcc
     1141 tcgcggcgtg tgtgccaccg cacagttcaa ccgaatcgcc catcctcacc atgcggacgc
     1201 tttcgccgta cttgtcttcg aagaagtgaa gggcgccttc ggcaatggcg gcctcgatcg
     1261 gcatctctcg cgtgacaatc ggcagatcgt cgaaaatcca gccctggaca agcttctcca
     1321 cctgctcgat ttcctgcctg gtcatcggcg ccatgtgcga gaagtcgaaa cgcaggcgtt
     1381 ccggcgatac ctgggatccc ctctggcgga catgcgttcc cagaacctgc ctgagagccc
     1441 tgtgcagcag gtgggtggtg ctgtgattcc gcctcgtgtc cagcctggcc ctgttgacca
     1501 cgaggccggc ttcgtgcccg accttcagga cgccggactc aaccttgccg acatgcatga
     1561 aacaatcgtc gccctgcttc ctggtatcgg aaacccggaa caggtaccct tcccccgaaa
     1621 tggtgccggt gtcgccgacc tgtccaccgg actcggcgta aaacggcgtc cgccggcaca
     1681 cgacgaccgc atcggccccg gccgacgcgg actccaccga cgaaccatcg acaaagattg
     1741 ccgcgacaac cgaggaaacc ggatttccgg cggcctcgtc atagtcgtaa ccgacaaact
     1801 cgctcttaat cgcgttgacc ctgcaatgct cggcgattcc gccccaggac gcatcgtgag
     1861 tcttccatga cgcacgaccg cgggccctct gctcctccat caaggcattg aaaccgtcgc
     1921 gatcgatacc catgccgttt tcttcggcga cgacctcggt aagatcaacc gggaatccgt
     1981 gcgtgtcata aagcgtgaag accgtctgtc cgtcaatgcc ggcgcgacct tcttccctgg
     2041 ccctcctgac ggcggcatca agcagttcca tcccggagga aagcgtcttc aggaagcgtt
     2101 cctcttcctg gcccgcgatc ctggcaatta cgtcggcgtc gcgctgaagc tcgggccagg
     2161 cagctcccat gacagtcgat acgaacaaaa ctatgtctgt gaaaaacggg ccgcggaaac
     2221 cgagcttgtg gccaaaccgg agcgcccggc gcatcaggcg acgaagcacg tatccacgcc
     2281 cctcgttttc ggggaacacc ccgtcggaga tgagaaaagc ggtggcgcgg gcatgatccg
     2341 cgatgacccg cagcgccgtg tcgttatcgg gatcctggcc gtacctgatg ccggcggcgg
     2401 ccgccgcgcg ctcgatgaga ggcatcagca gatcggtgtc atagttggtc cttacgccct
     2461 gaagaaccga tgcgattcgt tccagcccca ttccggtgtc gatcgaaggc gcgggaagag
     2521 gatgaaggac gccggcttcg tccctgttga actgcatgaa caccaggttc cagatctcca
     2581 tgaacctgtg tccctgatcg tgcatcgaag tcccggaata ggaaggcccc cgatcaacgt
     2641 aaatctcgct gcatgggccg catggacccg tgtcgcccat tgaccagaaa tcatccggat
     2701 cggcgatgat cctgtcgggg gcaacgcccg cctgggtgat ccagagctga cgggcctcgt
     2761 cgtcgtccgg atgaacggtt acccacaggt cggcgggatc aagtccgtaa accttggtca
     2821 gcagatccca ggcatagacg atggcgtcac gtttgaaata gtcgccgaac gaaaaatttc
     2881 cgagcatctc gaagaaggtg tggtgcctgg gagtgcgccc gacttcgtcg agatcgttgt
     2941 gcttcccgct cacgcgcagg cacttctggg ttgtcgtggc cctacggtat ccgggatcgc
     3001 gatagccggc gaaaacgtcc ttgaactgca ccatgccggc gttgacgaag agaagcgtcg
     3061 gatctgccgg tggaatcagt ccatggcttg ccacgatgcg atggccgcgc tcctcgaaga
     3121 attgaagaaa cttctggcgt atttctgcgg atttcacttt ttcctcttgt caccgggtcc
     3181 ggaaatggcc ccgtcacgcg gcgggcgtat cgtccaccgt aatcccgaac ttcgaataaa
     3241 cgtcgcgggt aacggccgcg agatcatcgg cgttgtcctt caggaaggcc atcgcctgct
     3301 cgcgtccctg acccagcctc ttgccggcat acgaatacca ggaaccgctc ttttcaagca
     3361 ccccgatctg gcccgccaga tcgacaagtt ccccaagcct gttgacgcct tcgccgtaca
     3421 tcaggtcaaa ctctgcctcg cggaatggcg gtgccatctt gttcttgacc accttgacct
     3481 tgaccctgga gccggtcgtc gtctggccat cggcttcctt gacggcctgt ccgcggcgga
     3541 cgtccaggcg aatcgacgca tagaacttca gtgcgttgcc gccggtcgtc gtttccggat
     3601 tgccgtacat gacgccgatc ttcattcgaa tctgattgat aaaaatcatg cacgtgttcg
     3661 acttggaaag aaccccggcc agcttgcgga gagcctgact catcagcctg gcctgaagac
     3721 ccacgtgcga atcgcccatc tcgccttcaa gctcggcctt cggcaccagg gccgcgaccg
     3781 aatcgatgac gacaaggtca acggcgttgg tgcggacaag ggtctcggcg atttcaagcg
     3841 cctgctcgcc agtgtccggc tgtgccagga gaagatcctc gaccgatatt ccaagcttcc
     3901 ttgcgtaatt gacgtcaagc gcgtgctcgg cgtcgatgaa cacggccagg ccgcccgccc
     3961 gctggcattc ggcgatggcc agcagcgtca acgtggtctt tccggaggat tcctggccat
     4021 agatttcgac tatccttccc ttcgggtatc cgccaatgcc cagcgcgatg ttcaacccga
     4081 gcgacccgga actgatgacg ggaaccctgc cgaccgtgct tgcgtcgccg gccatgcgca
     4141 ttatgctgcc ctttccgaac tggcgctcga tggtcgcgac cgcgacctcg atattgcgtt
     4201 ccctctgctc ggcggtttcg ccgaccttca gcttcgtatc cctgggttcc ttggccatct
     4261 gcatcctcca caggcggcgg gggccgccac aaatcacact tcctgcacgc cgcccgagtc
     4321 acgcacatgc cgggaaccgg cagcaatcgc aaggggggcg gtccttaccc tgcgacacaa
     4381 ggtcatcggg gtggttccga ccggctccag gattccaccg accaggccgc tgatggcaat
     4441 cctggacgga tactcaaaag acgccgcacc cgggatcgac gcgatttcgg aagcaccggg
     4501 catccatccg taaaccagtt tcaagcctcc ggacacctga tccacggact cgaccccgac
     4561 ccgctcaagt tcccgggcaa gcgcaccgga tatgaccccg atgaccgaat ccgttgacca
     4621 ggcacgggag attatcaaca ttgaaccgtc ggccagcctt tcaagcgaag gcggatcgat
     4681 gcaaatctga acatccctga aaccggacgc caccgacgac attcccatgc cgatgacctc
     4741 gtcaagggca cgcgggggaa gttcggccgt caggattgtg atcgcgcagg cggcgggcgg
     4801 catccagacg gcgccggcgc cccggcccag cctcttgcgg gtgtcgcggg taacgtcaag
     4861 cagaaggttc gcaaactgta tggaaggctc cagtactatc gccgccttcc tgacctgctc
     4921 agccttcctg ttgtcgcttt cgaccgtcat ctttcacctt gcatcccgga cgccatgaaa
     4981 atcggcagca gattaattca agccgtcccg gcgggcaaca tccaaataga acccccgacc
     5041 gccccgatcg acgcaccacc ttcgaaccat gtcgattgcc gcgatcgccg ccgcctcgcg
     5101 aacctcggcg cgtccacccg ggaagcggac cgtggcggat cgcacgcaat cggccgatgc
     5161 cagggcgaaa cacacggtgc cgaccggctt gtcagggtaa ccccccgaag gtccggcaat
     5221 acccgaaacc gcgaccgcca ggtcggttcc cagtacactt ctgcatcacg atgccatcgc
     5281 gcgggccaca tcctcggaaa ccgcccccga gttggcgatc cg
//
LOCUS       DAOPPA010000204         5293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17025_length_5293_cov_3.724322, whole genome shotgun sequence.
ACCESSION   DAOPPA010000204 DAOPPA010000000
VERSION     DAOPPA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17025_length_5293_cov_3.724322"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKH54_07290"
     CDS             <1..884
                     /locus_tag="PKH54_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99732.1"
                     /translation="NEGFADYFASTILDYPVLGEYSLGDDSRDLSEFKRCPDHYVGES
                     HYDGRIWSSTLWDIRTKLGITLADELAYRTLIATEMTSTFKTASESLLEIAGEISPEN
                     VDAIRAILEEHNVLDCSRVLEYGTDLAEKGYYLPGKMDSYAYEFQTSVASPFQYRIEV
                     PEGSNGFLVYFLAEDMYGYDEAPEVKLMVKRGEDPINWSYRDRPVPDADKSFEATRLN
                     SKDVMFTVAGSCVTPGVYHVQLLNKASYSIVSRVNTVSFPADFSELTWDTCQPADEGI
                     VEQGADAIESDIVDDTI"
     gene            987..2549
                     /locus_tag="PKH54_07295"
     CDS             987..2549
                     /locus_tag="PKH54_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC99733.1"
                     /translation="MRFVRKSVDARHRQRPVLVMSVDVELGPANSMRIDTLSLAPGIT
                     VEPVESVRYDLPQPGGAPLPSRPVVIGTGPAGLFCALTLARAGYAPIVLDRGDSVAER
                     TGCVGRFWGGGQLDCDSNVQFGEGGAGTFSDGKLTTRIRDPRCAEVLRQFVAAGAPPE
                     IEWEHLPHIGTDRLRVVIPAMTAMIRTLGGEVHFRCRVDSIVFGRSKEVTGVKCADGR
                     SFGTCAVVLATGHSARDTFEMLARQGVEMTPKPFAVGLRIEHSQKMIDAAQYGDFAGH
                     PALPPAVYKLAERIDDRRSAWSFCMCPGGSIINAASEADGVVTNGMSNLARDGRTANS
                     AIVVNVGHDDFADHGPLGGVEFQRSIERAAFAMVRAGRLPSGYGRSTPVTIRDGMPAT
                     TVGNLLGLSPPATASGGHGRGPARSGSAGGRDAAPYLPAFVTDTIARALPIMGRKIRG
                     FDNPGTVLVGPETRTSSPLRIPRGPDFQAVRNPGLYPSGEGAGYAGGIVSAAVDGIHV
                     AEAIITRFKPGY"
     gene            complement(2670..4232)
                     /locus_tag="PKH54_07300"
     CDS             complement(2670..4232)
                     /locus_tag="PKH54_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99734.1"
                     /translation="MDTGSDSIAADVATDHGGQIDSGTSDDLAHPDDARLDTHDVDDR
                     DDEIPGADSLVDPDSGNQDVDVIQIDTAADESSDPGVQPGDAQDDFIDVPVDVPHDTG
                     DQPCVTSTPCVDVQDCENGFRCNTKLSPPRCQKLYCGDFDTPCSDDFLCVSNRCKLDK
                     CTADCTGLECGPDPAYGISCGKCESGATCQSGACVCEPRHHSDCIDDDLFWFNSCGEA
                     GVMKQDCPFVCVNGACAGCDAVGKVDCSGVCTILGSNSNCSDCGDACDPTESCESGIC
                     TPVPDEGLVPAGKFWMGCSENDWYCDNDEYPIHEVNLSAFYIDRSEVTVSKYGECVTA
                     AKCTKPATTWSECSWNRQGKADSPVTCVTWDQAVQYCSWRSKRLCTEAEWEKAARGVD
                     AAIYPWGDTEATCDYAVMHDWDLSDDGCGTDDTWDVCGKSPIGDSPYGACDMAGNVNE
                     WVSDWYAEGYYDVSPASNPQGPATGSSHVIRGGSYINPHKPLRASSRSWYLTVLDREA
                     TALGFRCCRDAD"
     gene            complement(4453..>5293)
                     /locus_tag="PKH54_07305"
     CDS             complement(4453..>5293)
                     /locus_tag="PKH54_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99735.1"
                     /translation="FDRLEIASGERVSSQYEWYPTSAFSDNLPSAIRRAPDGELMLVN
                     EWRYVTYSNPNEDIYFARSFDEMLTWTNWVKAEHIGTEGTVYFSGSAVMDYNGVMWVA
                     ATTTWSGGSTVIIAFDGSAVTQREIVFSGLSFGSNHSGAVVFLNRSSGKLAFIALGSD
                     GVPKVRYNNAYQYGAWSDPEIISPDLVGGISIKANSELYSPLDYDDFVAVWNSSNLMY
                     LIEWDGDSTFRKASVPKYLSDPFMEGRVQLMPDGSPFVVQTYLPSDLENGYLVTMVKT
                     CRP"
BASE COUNT         1102 a         1623 c         1499 g         1069 t
ORIGIN      
        1 tgaacgaagg tttcgcggac tatttcgcca gcaccatcct ggactacccg gttcttggcg
       61 agtacagcct tggcgacgat tcacgcgacc tgtcagagtt caagcgctgc ccggatcatt
      121 acgtcggcga atcacactac gatgggcgaa tctggtcatc cacgctctgg gacatccgca
      181 ctaagcttgg catcacactt gccgacgaac tcgcttacag gacgctgatc gcgaccgaga
      241 tgacttcgac attcaagaca gcttcagaaa gtctgcttga aatcgccggc gagataagcc
      301 ccgaaaatgt cgatgcgatc cgggcaatcc ttgaagaaca caacgtgctt gattgctccc
      361 gcgtccttga gtacggcacg gatctcgctg aaaagggata ctacctgccc ggcaagatgg
      421 acagttacgc gtacgaattc cagacaagcg tggcatcgcc attccagtac cggatcgagg
      481 ttcccgaagg atcaaacgga ttcctggttt acttcctggc cgaggatatg tacgggtacg
      541 acgaggcgcc ggaagtcaaa ctgatggtta agcgaggcga ggatccgatc aactggagct
      601 accgtgaccg cccggttccc gacgccgaca agtcgttcga ggcgacccgc ctgaattcca
      661 aggacgtgat gtttactgtt gcgggaagct gcgttacacc gggcgtttac cacgtccaac
      721 ttcttaacaa ggcatcctat tcaatcgttt cccgtgtgaa cacagtgtcg ttcccggccg
      781 atttctcgga actgacctgg gatacctgcc aaccggcgga tgaaggtatc gttgaacagg
      841 gcgcggacgc gatcgagtca gacatcgttg acgatacaat ataattgtta agaatcaaca
      901 acatacgcgt tccgatttcg caggctggcg acatttccag tgccgcagcg aacgcaattg
      961 gcgtgaacga atcggcaatt tgcgaattga ggttcgttcg aaagtcggtc gatgcgcgtc
     1021 atcgccagcg ccccgttctt gtcatgtctg tcgatgtgga gcttgggccg gccaattcca
     1081 tgcggattga cacgctgtct cttgcaccag ggattactgt cgaacccgtc gaatcagtcc
     1141 ggtacgacct gccacagcct ggcggcgccc cgcttccttc gcgtccggtc gtcatcggca
     1201 ccggcccggc cggcttgttc tgcgctctta cccttgcgcg ggcagggtac gctccgatag
     1261 tccttgatcg cggtgattcg gttgccgaac gcaccggctg cgtcgggcgc ttctggggcg
     1321 gaggccagct tgattgcgat tcaaacgtcc agttcgggga aggcggcgca ggcacgtttt
     1381 ccgacggtaa gctgacaacc cgcatccgcg atcccaggtg cgccgaagtc cttcgacagt
     1441 tcgttgcggc cggggcaccc ccggaaatcg agtgggagca ccttccacac atcgggacgg
     1501 atcgacttcg ggtggtcatc ccggcaatga cagccatgat tcggacgctt ggcggcgaag
     1561 ttcacttcag gtgccgcgtc gattccatcg tgttcggcag aagcaaagaa gtcaccggcg
     1621 ttaaatgcgc cgatgggcgt tcgtttggca cctgcgcggt cgtcctggca accggacaca
     1681 gcgcccgcga cacatttgag atgcttgcgc ggcaaggggt cgaaatgaca ccgaagccgt
     1741 ttgccgtcgg cctgcggatc gagcattccc agaaaatgat cgacgcggcc cagtacggcg
     1801 atttcgccgg gcaccccgcc ctgccgccgg ctgtttacaa gcttgccgaa aggattgacg
     1861 atcgccgttc cgcatggtcc ttctgcatgt gcccgggtgg ttcaatcatc aacgccgcgt
     1921 cggaagccga cggcgtggtg acaaacggca tgtccaacct tgcacgtgac ggacgcacgg
     1981 caaacagcgc gatcgtcgtc aatgtcggtc acgatgactt tgcagatcac ggcccgctgg
     2041 gcggggtcga gttccagcga tccatcgagc gcgccgcgtt cgccatggtt cgggcggggc
     2101 gtcttccttc gggttacgga cgttcgaccc cagtcacgat tcgcgatggc atgccggcta
     2161 cgacagtcgg caacctgctt gggctctcgc cacccgccac ggcctccggc ggacatggcc
     2221 gcggcccggc acgctcgggg tctgccggcg gcagggatgc ggccccgtac cttcccgcgt
     2281 tcgttacgga caccatcgcc cgggcgcttc cgataatggg tcggaaaatt cgtggattcg
     2341 acaaccctgg cacggtcctg gtcggccccg aaacacgcac atcttcgcca ttgcggatcc
     2401 cccgggggcc cgacttccag gccgtccgca atcccggtct ttatccaagc ggggaaggcg
     2461 ccggttacgc cggcggcatc gtctcggcgg ccgttgacgg aatccacgtc gcggaagcca
     2521 tcatcacccg cttcaaaccc ggctactgag attttttccc gaaatcaaca cagaactgac
     2581 tcatggactg gtcggcaagc atgtttccag atccaaacca gtcctggcca tccggcaagg
     2641 ccgtgtcgtt caagcagaag agtgtcgact taatccgcat cgcggcagca acgaaagccc
     2701 agcgcggtcg cttctcgatc gaggacggtc aggtaccatg atctgctgga ggcgcgcagg
     2761 ggcttgtggg gattgatata tgacccgccc cggatgacat ggctgctgcc ggtggccggg
     2821 ccctggggat tactggcggg cgatacgtcg tagtatcctt ccgcatacca gtcagaaacc
     2881 cactcgttca cattgcccgc catgtcgcag gcgccgtacg ggctatctcc aatgggtgac
     2941 ttgccgcaga cgtcccaggt atcgtccgtt ccgcatccgt catccgacaa atcccaatcg
     3001 tgcattacgg catagtcgca tgtcgcttcg gtatcgcccc atggatatat ggccgcatcg
     3061 accccgcggg cggccttttc ccactcggct tcggtgcaca gacgctttga acgccaggaa
     3121 cagtactgaa cggcctgatc ccaggtgaca catgttacag ggctgtcggc cttaccctgt
     3181 cgattccaac tgcactcgct ccaagtcgtc gcgggcttcg tgcacttggc tgccgtcacg
     3241 cattcgccat acttggaaac ggtgacttcc gacctgtcga tgtaaaaggc cgaaagattc
     3301 acttcatgaa tcggatattc atcgttatca cagtaccagt cgttttcgct gcaccccatc
     3361 caaaattttc ccgcaggcac aagaccttca tctggtaccg gcgtgcatat gccggattcg
     3421 catgattcgg ttggatcgca ggcgtcgccg caatcggagc agttcgaatt tgaaccaaga
     3481 atggtgcaaa cgccggaaca gtcaaccttg ccgaccgcat cacatccggc acaggccccg
     3541 ttaacgcata cgaacgggca gtcctgcttc atgacgccgg cctcgccgca cgaattgaac
     3601 cagaaaaggt cgtcgtcaat gcagtctgaa tgatgcctcg gctcacacac gcatgccccg
     3661 ctctggcaag tcgccccgct ttcgcacttg ccgcaggaaa ttccatacgc cggatccggc
     3721 ccgcattcca ggccggtgca gtcggcggtg catttgtcca gcttgcagcg gttcgacacg
     3781 cacagaaaat cgtcggagca aggcgtgtca aagtcgccgc agtacagttt ctggcagcgc
     3841 ggcggggaca atttcgtgtt gcagcggaac ccgttttcgc aatcctgcac atcgacgcac
     3901 ggcgttgaag tgacgcaagg ctggtcgccg gtgtcgtgcg gcacgtcaac cggcacatcg
     3961 atgaagtcgt cctgcgcatc ccccggctgg actccgggat ccgacgattc atccgcagcc
     4021 gtatcgattt ggatgacatc gacgtcctga ttgcctgagt cgggatccac gaggctgtcc
     4081 gcaccgggaa tttcatcgtc ccggtcgtcc acgtcatgcg tgtccaaacg ggcgtcgtct
     4141 ggatgcgcca gatcatcact ggtgccgcta tcgatctgcc cgccatgatc ggtggcaacg
     4201 tctgcagcga ttgaatcgga accggtgtcc atggcagggc cctggccccc accacatccc
     4261 acgaataata cgaatgccgg aaggatcatc cataatccga tcacgcgacc agaaaaacaa
     4321 gcgaactgtt tcataatgcc accatcccct cttcccggat atcaggagtt ccccgttccc
     4381 gcgggatcat ttcgcccttg cgggggcgga cagacaaagc aacaggccga ccaggccgga
     4441 ctcagatgcg gcctatggcc tgcatgtttt caccatcgtg accaggtacc cgttttccag
     4501 gtcggatgga aggtacgtct ggacaacaaa cgggcttcca tccggcatca actgaacgcg
     4561 cccttccatg aacggatctg aaaggtactt tggaacggac gccttacgga aggtgctatc
     4621 gccgtcccac tcgatcaggt acatcagatt cgaactgttc cacaccgcca cgaaatcatc
     4681 gtagtcaagg ggggaataca attccgaatt ggccttgatg ctgatgccgc cgacaagatc
     4741 cggcgatatg atttcggggt cggaccacgc accgtattga tacgcattgt tgtaccgaac
     4801 cttcgggaca ccgtcagacc caagggcgat gaaggccagc tttcccgaac tgcggttaag
     4861 gaaaacgacc gccccggagt gatttgagcc gaacgaaagc cccgagaaca cgatctcgcg
     4921 ctgggtaacc gcgctgccgt caaaggcgat aatcacggtc gaaccgccgc tccaggtcgt
     4981 ggtcgcggca acccacatca cgccgttgta atccatgacg gccgagccgg aaaaatagac
     5041 ggtgccttcc gtgccgatat gctcggcctt gacccagttc gtccaggtca acatttcatc
     5101 gaacgaacgg gcgaaataga tgtcctcgtt cggattcgaa tacgtcacat accgccactc
     5161 attaacaagc atcagttcgc catcaggtgc ccgcctgatc gcggaaggaa ggttgtcgga
     5221 gaaagctgag gtcggatacc actcgtactg gctgctgacg cgttcaccgg aggcgatttc
     5281 caagcgatcg aaa
//
LOCUS       DAOPPA010000205         5288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17048_length_5288_cov_4.135677, whole genome shotgun sequence.
ACCESSION   DAOPPA010000205 DAOPPA010000000
VERSION     DAOPPA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17048_length_5288_cov_4.135677"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2921)
                     /locus_tag="PKH54_07310"
     CDS             complement(<1..2921)
                     /locus_tag="PKH54_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99736.1"
                     /translation="MKASSGTRSSSWRIQVMVPALLALLPTVSCGNAPDPEADVTADQ
                     GPAEVTYDEGTLPDGVIDNGAPDTGRDLIAVDIQPDGIVPGGFGAPCDDYDDCDSRLC
                     VEAEGGSICTKLCLQEGCPEGFVCSGITNTYPDALFLCMPVFSKVCSSCTFDNQCAGG
                     KCVQFPEGKFCTVPCETTACPSDYQCVDADGGQGRFCVPPSGSCLCRKKDEGTRRACE
                     KTNEIGTCYGYESCDPEVGFTACDASLAKAEECNGLDDDCDGSPDDGLVEGESCLVEN
                     EFGACQGTSVCFGLKGWICGAKEPEAETCNGIDDDCDGETDEDFKVDGAYGSLENCGA
                     CNKSCIGIFPNATVDCDATGPVPRCIVTDCDDGFFKLNDYQCIPLTSTICNTCVIDSD
                     CIIEGARCVEFADGGRYCGHPCENSGNCPTGYSCVAVPGGDDQCLPDSGSCACDTPDP
                     NITRVCQVSWQENPESPLYTCSGIQRCGEGGWSDCELPAEQCNGQDDNCNGEIDEGFL
                     TDGRYTSDTNCGVCANNCTTMSVTNGFGVCDTSRTIPDCKVECNNGFFNVDGNPANGC
                     ECQYKGAVDLPGGGDDDCDGIDGSTEGAVFVAKWGADTNPGTIEQPVLTIARGMQLAD
                     GAPARDVYVATGIYTEPLNIREGVSVYGGFSADFKILNPIAYETVILGQEPIALLPGA
                     VNAVSINGQAAGSTVLSGFVVIAYNNRTAGQNSIGIYIKDCDSSLAVRDCRIVGGLAG
                     NGAAGGLGVSGEDGVGGAAGLAAYDINTANCGTTTRAGGQGGTHSCGTTPVGGGSGGT
                     AICPDFDEDTTGCATGPTIFNPDRVILQTRKSNENGTGGLNGGGAGGIAGLDSIIDSD
                     CSYSGSCGSCSVPSETRTGAAGMPGNTGTDGGGGNGCTNTSGNVVDGLWVPAQATGGG
                     TGAHGSGGGGGGAAGGVETLNCGPSDGGNRGYTDLGGSGGGGGSGGCGATGGTPGGSG
                     GG"
     gene            4815..>5288
                     /locus_tag="PKH54_07315"
     CDS             4815..>5288
                     /locus_tag="PKH54_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99737.1"
                     /translation="MNRVVLLSGCVAVLVISACGGGGVDVLDAQDRDNVVDYLQPDSL
                     VDEGDPVDGGVDANAPEVDAVVDRDADAATISDSDAEGLTDGFESDGDDDAVPSDAPD
                     APDADSGEEPGGFGWPCDDDSDCLYGPCIVTDDGRVCSSECSDSTCPEGFDCVPSI"
BASE COUNT         1070 a         1581 c         1590 g         1047 t
ORIGIN      
        1 ccgccgccgg acccgccagg cgttcccccg gtcgcaccac agccgcccga gccgccgcca
       61 ccgccagaac cgccgagatc ggtatagccg cggttgccgc cgtccgaagg tccgcagttg
      121 agtgtctcga cgccgccggc cgcgccgccg ccaccaccgc cggagccatg tgcgccggtt
      181 ccacctccgg tcgcctgggc gggaacccag aggccgtcaa caacgttgcc cgaagtgttc
      241 gtgcagccgt tgccgccgcc cccgtcggtg ccggtgtttc caggcatgcc ggccgcgccg
      301 gttctggttt cggaaggaac cgagcacgaa ccgcaggagc ccgaatagct gcaatcgctg
      361 tcgatgatcg agtccaggcc cgcgatgccg ccggcgccgc ccccgttcag cccgccggtt
      421 ccattttcat tcgacttgcg ggtctgaagg attaccctgt cggggttgaa tatcgtcggt
      481 cccgtcgcgc atccggtggt gtcctcgtca aagtccgggc agatggccgt tccaccgctg
      541 ccgccgccca cgggcgtggt gccgcatgag tgggttccac cctggccgcc cgccctggtc
      601 gtcgttccgc agttcgctgt attgatgtca taggccgcca ggccggcagc accgccaacg
      661 ccatcctcac ccgagacgcc gaggcccccg gcggcgccgt tgccggccag tccgccaacg
      721 atacggcagt cacgaacggc aagcgacgaa tcgcaatcct tgatgtaaat gccgatggag
      781 ttctgcccag ccgtgcggtt gttgtaggcg ataacgacga agcctgaaag cacggtggat
      841 ccggccgcct gaccgttgat gctgaccgcg ttgaccgcgc ccggaagcag cgcgataggt
      901 tcctggccaa ggatgactgt ttcgtaggca atcgggttca gtattttgaa atcggccgag
      961 aatccgccat agacgctgac gccttcgcgg atgttcagcg gttccgtgta tatgccggtg
     1021 gcgacataaa catcgcgggc tggggcgcca tcggccagtt gcatgcccct tgcaatcgtc
     1081 aataccggct gctcgatggt tccgggattc gtatcggcgc cccatttggc cacgaaaacg
     1141 gcgccttccg tgcttccgtc gatcccgtcg cagtcgtcgt cgccgccacc gggcagatcg
     1201 accgccccct tgtactggca ttcgcatcca ttggccggat tgccgtcaac gttgaaaaat
     1261 ccgttgttgc attcgacctt gcagtcggga atcgtcctgg acgtgtcgca taccccgaag
     1321 ccgttggtca cgctcatggt cgtgcagttg ttggcgcaca cgccgcaatt ggtgtcggat
     1381 gtgtaacggc cgtccgtaag gaagccctcg tcgatttcgc cgttgcagtt gtcgtcctgg
     1441 ccgttgcact gttcggcggg cagttcgcaa tcgctccagc caccctcgcc gcatctctga
     1501 atgcccgagc acgtgtaaag cggagactcc ggattttcct gccacgaaac ctggcagacc
     1561 ctggtgatgt tggggtcggg cgtatcgcat gcgcaggaac cggaatcggg caggcactgg
     1621 tcatcgccgc caggaaccgc cacgcatgaa tagccggtcg ggcagttgcc gctgttttcg
     1681 caggggtgcc cgcagtaacg gccgccgtcg gcgaactcga cgcacctggc gccttcgatg
     1741 atgcagtccg aatcgatgac gcatgtgttg cagattgttg atgtaagcgg aatgcactgg
     1801 taatcgttaa gcttgaagaa tccgtcatcg cagtccgtca cgatgcagcg cggaaccggg
     1861 ccggtcgcgt cgcagtcaac cgtggcgttc gggaagattc cgatgcagga cttgttgcat
     1921 gccccgcagt tttcaagcga accataggcg ccgtcaacct tgaagtcctc gtccgtttcg
     1981 ccgtcgcagt cgtcgtcgat gccgttgcat gtttcggcct cgggctcctt cgccccgcag
     2041 atccagcctt tcagcccgaa gcagacgctc gtaccctggc aggcgccgaa ttcgttctcg
     2101 acaaggcacg actcgccttc aacaagtcca tcgtccgggg aaccgtcgca gtcgtcgtcc
     2161 agtccgttgc attcctctgc cttggccagg cttgcgtcgc aggcagtgaa cccgacttcc
     2221 gggtcgcacg attcgtagcc gtagcatgtg ccgatttcgt tcgtcttttc gcaggcccgc
     2281 ctggtgccct cgtccttctt gcggcagagg catgagccac tgggaggcac gcagaagcgt
     2341 ccctggccgc catcggcgtc aacgcactgg taatccgacg ggcaggcagt cgtttcgcag
     2401 ggaaccgtgc agaactttcc ctccgggaac tgcacgcact tcccacctgc gcactggttg
     2461 tcaaaggtgc atgacgaaca gaccttcgaa aagaccggca tgcagaggaa gagcgcatcc
     2521 ggatacgtat tggtgattcc cgagcagacg aatccctcgg gacatccttc ctgaaggcag
     2581 agcttcgtgc agatgcttcc gccttcagcc tccacgcaca gccggctgtc gcagtcgtcg
     2641 taatcgtcgc aaggtgcacc gaagccgccc ggaacaatgc catccggctg gatgtcgacc
     2701 gcaatcaggt cgcgtccggt gtcgggcgcg ccgttgtcga ttacgccatc ggggagggtg
     2761 ccttcatcgt aggttacttc cgccggtccc tgatccgccg tcacgtccgc ctccggatcc
     2821 ggagcgtttc cgcatgaaac cgtcggaaga agcgccaaca gtgccggaac cattacctgg
     2881 atccgccatg aagatgatcg ggttcctgat gaagccttca ttgggtttta cctccggtgg
     2941 ctttttcaac tgattactga atccggatac atccttatca tttatcaact ttcgtcgccc
     3001 atttgcaaca ggttccgcat cgaatggacg cggcttcgga tgtgaaatgg cggcgaacag
     3061 ggaccaaccc ttgttttccg cgataagttc aagtgaatcg atattgctcg tgatgttgcc
     3121 ggagtcttcc ttcctgacaa ggatgtagtc atagtaacgc caggccggat ttgacgtgaa
     3181 atcgcgcccc atgatcggcg gaatcatgcc cggcttgtac tgaatggggt tatgtggccg
     3241 ttcggcgaac tgcatgtcgc tgacgccacc tttcaggacg ccgtaaaggt gatggatatg
     3301 ccaaagcgca ccggcgttga cgacgcggct gtcctggttg ttcgcgattt tcagaagccg
     3361 acgtccacca gccgccttgt cgaacatctc ggcgtatccg gcaacttccc tctggaacct
     3421 gatgaagccc gaggaaacca ccacgattcc ggcgattgac agggcgaccg cgccggccat
     3481 cacaagggcg acaaggcgtc ccgaactgcg tgtgaagatt ggccaggcca cgaaaaacat
     3541 gaaggccgga aggatgtgcc gcagggaaac gaccgcctgg gcctggatgt gatgcgggag
     3601 tatcaggacg gacgcaccaa ggaccagcgt caggatttcc aggtcgaaaa atttcagccg
     3661 ccgcttctga tccgcgggaa tttccggcgc ccgcctgacg ataagaagta cgagcacaag
     3721 aagaatcatc agaacgaaaa cggtgtcatc cgactgtctt cggaagtaat tgccgatgta
     3781 gttgaaaaaa ttggaaagta tctgcgacga gctatagtac ttcggcccga aaaagtcatc
     3841 ggccgaaccg aaaggcatgc ccgatgaaga aggggtgttc tcgatgaaat agacccagaa
     3901 ccaggggacg aaaaacgtca ttgacggcgc gaagggcgcg atccggaaga ccgagtcgcg
     3961 aagtcccctg acatgcagca gccagagtgt tccgaaaagc attccggcca tcaggaaaac
     4021 atgggcatgc gtgaagtaca tccacacaag catcacggcc gcggcaattc ccggcccgat
     4081 cgcccgcctg acgtcgtcga tggcaaggaa ccgcctgtat gcgccaaggc cggcgaacag
     4141 catcggcatt gcaagcgcga aggaaacaaa gccaagataa aacatggcgt tgaacacgaa
     4201 cggaaatgaa aagagcgcca gccactttga gcgcccgaac gaccgcagaa ggaagaggta
     4261 cgacgccggg aatgcaagga catatatcgt cagtaccacc ttgttagcga attccaggct
     4321 tggcgtcacg aacgcgataa ggtgcgtgaa gtagtagtag aagaggtttg gcttgggcga
     4381 gtcgggcaga atgaagtagt ccgggtagat cgagccgggc gcccagtaat tgtggattac
     4441 gtcaacaagc gccagatgct gccagatatc ctgcatcggc aggatcctga tcagccagat
     4501 cggaattgtg ctggccacgg aaagaaggac gaagatcacc acgaagatgc gatcccgaac
     4561 cttgtccccc ggcattttta atacaaatcc cacgcgggca ttataggaca gtgcggcagc
     4621 cactgaaaag ggttttgcca cctcgcatgc cccctgacca ttgaacttga acaggtttct
     4681 gatatcgtgc gcacggccgc gaaaaaggct acaatagcgg gaaattgtcg aagccggcat
     4741 gccttgtccg aaattgggcc ggtcgaatgc cgaaggcttg actgattgaa aagaatacaa
     4801 cggcgggttt gtcgatgaac agggtcgttc ttttgtccgg atgcgttgcc gtgctggtca
     4861 tttccgcctg tgggggtggt ggcgtcgatg tcctcgatgc acaggaccgg gacaatgtcg
     4921 tcgactacct gcagcccgat tcgttggtgg atgaaggcga tcccgttgac ggcggcgtgg
     4981 acgcgaacgc cccggaagtc gacgccgtgg ttgatcgcga tgccgatgcc gcgacgattt
     5041 cggattcgga tgccgaaggc cttaccgacg ggttcgagtc tgacggggac gacgacgcgg
     5101 ttccatcgga tgcaccggat gcaccggacg ccgattccgg tgaagagcct ggcggcttcg
     5161 gctggccatg cgacgatgat tccgattgtc tgtacgggcc gtgcatcgtc accgatgatg
     5221 gtcgcgtctg ttcgtcggag tgttcggatt ctacgtgccc ggaaggattt gactgcgttc
     5281 cttcaatc
//
LOCUS       DAOPPA010000206         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17133_length_5263_cov_4.078533, whole genome shotgun sequence.
ACCESSION   DAOPPA010000206 DAOPPA010000000
VERSION     DAOPPA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17133_length_5263_cov_4.078533"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="PKH54_07320"
     CDS             <1..1649
                     /locus_tag="PKH54_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99738.1"
                     /translation="HVFVESCGESAGLSPMGGPMKISASQIKSFNDMKGVAGHELVHV
                     FQGQYFLTGKTGRLAHLVAGVGSWLRFIEGSANYFSARAQSLSGAARDTLYGRNEDGK
                     YMSLGLSAGNDQSYYMTAHFLDFIADRAGIGVVPAVLRAGSGSNSTAALSRALLDSPR
                     GDGIGTILEAYAQALVKTPETGSGLHLHLVANLLNHQMDAELGYLSGTSFSNSTTFLT
                     LKRLLPPLSLAYAEARDTTATDSLLVVEALPTNPSSVATFTWDRLGTTDAVFADALPL
                     EDYSGRTLTVKNFGAGQVKSGFSQIIVNNDLSRHHEIDFKYYILIPLPVIGQDDGSAT
                     VDTSQLGNIPADYIKELRLLRMTDAGTYELLTKFSPAGGQTVVSNRAILKDDLLEVQT
                     VDRHGNEWPEFDMGEAVTVYVGCHDFVDGADVWGCEKLDWVGQDTESTAFIDAATVKC
                     LNNNYRQYYAMAFDEIDMCRDWCDDRASGGGTCLTPGEGGEGLCGSIEPGNDFCLDGE
                     CWNCCADCEEPAIYLFSCMYGCQTGVIELHGIKTVVCECN"
     gene            1863..3323
                     /locus_tag="PKH54_07325"
     CDS             1863..3323
                     /locus_tag="PKH54_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC99739.1"
                     /translation="MKPIPFVLAVFVATMAAAGCGNGENGNDVMSDVKAGDVAPEAAA
                     DTVEDSLQPIDATGEATTDGAVQDALPEDTADASIPDIPSDTTGDVPVDTAPEFHWQP
                     GCTGVPTGASWTLVEDSTFKRGPLIQMSDRDTVTVVWRTATVTTDEGCVDYTVNDQPL
                     TECGLPDKYGQYEITLDDLPAATEIHYSARVGETRTTDLTFRTMPDTPAPMKFAVFAD
                     AHCNIENLRKMTAIALAEGVDFAFGIGDFVHQVEVAQYDETFLGFQNLGSRVNIWTVI
                     GNHDTGADPSNHEYEFFNSFVSPIGIPEEVEDGYGEGYWDRRIGNVWLGGGWVRDFYL
                     STPETEWGEVAFFKKRFESEEYKTAQWRLFFIHEPPYCQGWGGCSYSGELCLRTSLVP
                     LAAANGIQAMFHGHMHGYEYGVTEGVHTILPGGLGGSLDDTCEPTPELPQPWTFNYVH
                     NFAIVEANCDKLVVRFMNLDGGEYDRLEIPYVAPQE"
     gene            3386..4219
                     /locus_tag="PKH54_07330"
     CDS             3386..4219
                     /locus_tag="PKH54_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC99740.1"
                     /translation="MNSTISQALKIARFDLGTSIRTSKAVVTMAVYAGLALLVAAVTT
                     FLQNKLLAEPGVQEGLKTQMDQIQQVISAALGNDTLLANHLVNMPVALMVFFWLTQSF
                     LPLLIAVISSDMLNREIRTKSARFVLLRASRSSLVIGKWLSHITILIAAIVLSWIVFL
                     GYLAFNLNGFNLVQSIPLILKFFGFTLLIGFCYLGLTSLMSSLVDGSGTALLLTIVVL
                     LFLAGLAQSDMFGWASPSHYRYKIWSPRADEILAGVGAYVLFGSLFLSAAWQRFLRRD
                     I"
     gene            4216..5166
                     /locus_tag="PKH54_07335"
     CDS             4216..5166
                     /locus_tag="PKH54_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC99741.1"
                     /translation="MNPTQQHNSGGIAVQTENLSKAFGNLIAVDRLNLQIPTGSIFGL
                     LGPNGAGKTTTFSMLCGFVKPSSGSATVLGYPLKEFPFHAGRLSALPQDARFNPDRTV
                     LQSMIFLGSLVGLPPQEAREQAAQLLRAVDLADAARMKGAHLSHGMSKRFGIAQAFLG
                     RPDLVMLDEPTAGLDPRNAFTVRQMIRALAGTATVVVSSHNLAEVQDLCTHAAIISQG
                     KLVSSGRMDDLTSRNQQVMITISNLDSAIQARIEAIPGVVGVMPGGNDGELKVMFKTT
                     ADQKVEDAVSAIIAGAIAAGASVSGVRRGQDLEQRYLEVT"
BASE COUNT         1054 a         1642 c         1532 g         1035 t
ORIGIN      
        1 gccatgtatt cgtggaatca tgcggagaat cggccggact cagcccgatg ggcggcccga
       61 tgaaaatttc cgccagccag atcaagtcct tcaacgacat gaagggcgtg gccggccacg
      121 agctggtgca cgtgttccag gggcagtact tcctgactgg caaaaccggc cggctggcgc
      181 acctggttgc cggggtgggc tcatggttgc ggttcatcga aggctcggcg aactacttca
      241 gcgccagggc ccagtcgctt tccggggccg caagggacac gctttacgga cgaaacgagg
      301 acggcaagta catgagcctg gggctttccg ccggaaacga ccagagttac tacatgacgg
      361 cccacttcct ggacttcatc gccgaccgcg cgggcatagg tgtcgtcccg gccgtcctca
      421 gggcgggctc cggttcaaat tccacggctg cactttctcg cgcgcttctg gattcacccc
      481 gcggcgacgg gattggcacc atccttgagg cgtacgcgca ggccctggta aagacgccgg
      541 agaccggatc gggcttgcat cttcacctgg tcgcaaacct gcttaaccac cagatggacg
      601 cggaattggg ctacctttcg ggaacttcat tctcaaattc aaccaccttc ctgaccctga
      661 aacgactgtt gcccccgctg agccttgcat atgcagaggc gcgcgacacg actgcgaccg
      721 attccttgct tgtggtggag gccctgccga cgaacccgtc ctcggttgcg acattcacct
      781 gggatcgtct gggtacgacc gacgcagttt tcgcggacgc actgcccctg gaggactact
      841 ccggaaggac actgaccgtg aagaacttcg gcgccggtca ggtcaaatcc ggattttccc
      901 agatcatcgt caacaacgac ctgtcgcgcc accatgaaat cgacttcaag tactacatcc
      961 tgattccgtt gccggtcatc ggccaggacg atggcagcgc cacggtcgat acgtcgcagc
     1021 ttggaaacat cccggccgat tacataaagg aattgcgcct tctgcggatg accgatgccg
     1081 gtacatacga actgctgacg aaattctccc cggccggcgg acagaccgtc gtttcaaacc
     1141 gggcaatcct gaaagacgac ctgcttgaag tccagacggt cgaccggcac ggcaacgaat
     1201 ggccggaatt cgacatgggc gaggcggtga ccgtttacgt gggctgtcat gacttcgtcg
     1261 acggcgcgga cgtctggggt tgcgaaaaac tggactgggt cggccaggac acggaatcga
     1321 cggcgttcat cgacgcagcc acggtcaagt gcctgaacaa caactaccgg cagtattatg
     1381 cgatggcgtt cgacgaaatc gacatgtgcc gcgactggtg tgacgaccgg gcttccggcg
     1441 gcggcacctg ccttaccccc ggcgaaggcg gcgaaggact ttgcggcagc attgaacccg
     1501 gcaacgactt ctgcctggac ggcgagtgct ggaactgctg cgccgactgc gaagaaccgg
     1561 ccatttacct gttttcatgc atgtacggct gccagaccgg agtgattgaa ctccacggca
     1621 tcaagacggt cgtttgcgaa tgtaactaat ccggggcggg agtccattct cccggacttc
     1681 ggatgaacga aatcgaggca agcgtgctgt cggccggcac aacaatctgc ctcctctcca
     1741 gttcgattat tgacgcatgc cgtcattggg ccaacattcc ggacgtcatt tctgatagaa
     1801 tccgcggcat atgcgccccg gccgcgtccg gggacctttc agtcgttttt cgaggcccgc
     1861 cgatgaaacc gattccattc gtccttgctg tatttgttgc cacaatggcg gccgccggtt
     1921 gcggcaatgg tgaaaacgga aatgacgtca tgtcagacgt gaaggccggc gatgtcgccc
     1981 ccgaagcggc tgccgacacc gtcgaggaca gcctgcagcc gattgacgcc accggcgagg
     2041 cgaccaccga tggcgcggtt caggatgcgc tgccggaaga caccgccgac gcctcgatcc
     2101 cggacatccc gtccgacacg acgggcgacg tccccgtgga caccgccccg gaattccact
     2161 ggcagccagg ttgcaccggc gtgccgaccg gtgcgtcgtg gacacttgtt gaagacagca
     2221 cgttcaagcg cggccccctg atccagatgt ccgaccgcga caccgtcacg gtcgtgtggc
     2281 gaaccgcgac ggtgacgacc gacgaaggtt gcgtcgatta caccgtcaat gaccagcccc
     2341 tgaccgaatg cgggcttccg gacaaatacg gccagtacga aatcacgctt gacgaccttc
     2401 cggccgcgac cgaaatccat tactcggcac gcgttggcga aacccgtacg accgacctga
     2461 cgttccgcac gatgccggac accccggcgc cgatgaagtt cgcggttttc gccgatgccc
     2521 actgcaacat cgaaaacctg cgaaagatga cggcaatcgc gcttgccgaa ggcgtcgatt
     2581 tcgctttcgg tatcggcgat ttcgtccacc aggtcgaagt tgcccagtac gatgagactt
     2641 tcctcgggtt ccagaatctt ggaagccgcg tgaacatctg gaccgtcatc ggaaatcacg
     2701 acaccggggc ggatccttcg aaccacgagt acgaattctt caacagcttc gtcagcccga
     2761 tcggcattcc cgaagaggta gaggacggtt acggcgaagg ctactgggat cgccggatcg
     2821 gaaacgtgtg gcttggcggc ggatgggtac gcgatttcta tctttcgaca cctgaaaccg
     2881 aatggggcga ggtcgccttc ttcaagaagc gcttcgaatc cgaggaatac aagaccgcgc
     2941 agtggcgcct gttcttcata cacgaaccac cgtactgcca gggctggggc ggctgcagct
     3001 attccggcga attatgcctg cgcacatccc ttgtgccgct ggccgccgcc aacggaatcc
     3061 aggcgatgtt ccacggccac atgcacggtt acgaatacgg tgtgaccgaa ggcgtccaca
     3121 ccattcttcc cggcggcctg ggcggctcgc tggacgacac atgcgaaccg acgcccgagc
     3181 tgccgcagcc atggacattc aactacgtgc acaacttcgc gattgtcgaa gccaactgcg
     3241 acaaactggt tgtccgcttc atgaaccttg acggcggcga gtacgacagg ctcgagattc
     3301 cgtacgtggc gccgcaggaa tgaaacggga ataccgcatc ggcgcgccgt gttaatatcc
     3361 gtcatcgatt tccgggagca ttcccttgaa cagcaccatt tcacaggccc tgaagatcgc
     3421 caggtttgac ctggggacct cgataaggac cagcaaggcc gtcgtcacca tggcggttta
     3481 tgcgggactc gccctgctgg tcgccgccgt gacgaccttt cttcagaaca agctgcttgc
     3541 ggaacctggc gtccaggaag gcctgaagac ccagatggat cagatccagc aggtaatttc
     3601 ggccgcgctt ggaaacgaca ccctgctggc gaaccacctg gtcaacatgc cggttgccct
     3661 gatggtattc ttctggctga cccagtcgtt ccttccgctt ctgattgccg tcatcagcag
     3721 cgacatgctc aaccgcgaaa tcaggacgaa atcggcccgg ttcgttctgc tgcgggcctc
     3781 gagatcctcg ctggtcatcg gcaagtggct ttcccacatc accattttga ttgccgcaat
     3841 cgttctttcc tggatagtgt tccttggcta cctggcgttc aacctgaacg gcttcaacct
     3901 ggtccagtcc atccccctga ttctgaaatt cttcggattc accctgctga tcggcttctg
     3961 ctacctggga ctgaccagcc tgatgtcctc actggtcgat ggctcgggaa ccgcgctgct
     4021 gctgacgatc gttgtgctgc tcttcctggc gggactggcc cagtcagaca tgttcggatg
     4081 ggcgtcgcca tcccactacc gatacaaaat ctggtcaccg agagccgatg aaatcctggc
     4141 cggcgtcggg gcatacgtgc tgtttggaag cctgttcctt tcggccgcct ggcagcgttt
     4201 cctcaggagg gacatatgaa cccgacccag cagcataatt cgggcggcat tgcggttcag
     4261 acggaaaacc tgtccaaggc attcggcaac ctgattgcgg ttgacaggct gaacctgcag
     4321 atcccgaccg gttccatctt cggcctgctc ggaccgaacg gggccggaaa gacaaccacg
     4381 ttttcgatgt tgtgcggctt cgtcaagcca tcttccgggt cggcgacggt cctgggttat
     4441 ccgctgaagg aatttccatt tcacgccgga cgcctttcgg cgcttccgca ggacgccagg
     4501 ttcaatcccg acaggacggt gctgcaaagc atgatttttc ttggcagcct tgtcgggctg
     4561 cccccgcagg aagcaaggga acaggcggcg cagcttcttc gcgcggttga tctcgccgac
     4621 gcggcccgca tgaagggtgc ccacctgtca cacggcatgt cgaagcgctt cggcattgcc
     4681 caggcgttcc ttggcaggcc tgaccttgtc atgcttgacg aaccgaccgc cggccttgat
     4741 ccgcgaaacg cgttcacggt gcgccagatg atccgggcgc ttgccggcac cgcgaccgtc
     4801 gtcgtatcaa gccacaacct tgccgaggtc caggaccttt gcacccacgc ggccatcatt
     4861 tcacagggca aactggtttc ctccggccgc atggacgacc tgacctcacg caaccagcag
     4921 gtgatgatca ccatttccaa cctggactct gccattcagg cccgaatcga ggccattccg
     4981 ggcgtcgtcg gcgtgatgcc gggtggaaac gatggcgaat tgaaggtgat gttcaagacc
     5041 acggctgacc agaaagtgga agacgccgtt tccgcaatca ttgcgggggc aattgccgcc
     5101 ggcgcgtcgg tcagcggcgt caggcgcggc caggatctgg aacagcgcta ccttgaagtg
     5161 acctgaccgg gatcgattac gacgccgccc ctgatcccga caacccttga cgccacccga
     5221 catgcgccgg caacgatttc ttccgggttt cccgcgacgc cct
//
LOCUS       DAOPPA010000207         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17317_length_5220_cov_4.116167, whole genome shotgun sequence.
ACCESSION   DAOPPA010000207 DAOPPA010000000
VERSION     DAOPPA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17317_length_5220_cov_4.116167"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKH54_07340"
     CDS             complement(<1..840)
                     /locus_tag="PKH54_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99742.1"
                     /translation="MTKSRRLSIFLAAAMMTLASAAGCGGGPNTSPDTISGDIGADSL
                     DSGIPVDSIDTVVASNMTQAGLPVKVDCIGSGWEPGLARILVWDPKKATIDFGQEGEP
                     QEGDNPEITKLDPDTMELPEGIELEDGRLTFTVTGSFNVTCYAPDAGMIDLQPERISV
                     DPGMAVQIDTEATPTLVKAGAWVHVTCTGKDRWDNPITSGFFPAVTPQNGVKTSGLDV
                     QFIAVGTFDIACGIENTTMLDSTPVQVTVEPNNARKIYTKLDPQSIVAGESSRVDCEV
                     RDFY"
     gene            complement(922..2034)
                     /locus_tag="PKH54_07345"
     CDS             complement(922..2034)
                     /locus_tag="PKH54_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99743.1"
                     /translation="MTPAGLSEIKVIFSDTHLGAGHAPGIVNPFEDFHHDRELSDLIR
                     HYSTGEWADVPVELFINGDFLDTIKIPFEGHHTTEITEEASAWKVARCIKGHPAVFDA
                     LAAFAGVPGHTITYNAGNHDIDVAWPLVQKVLRARLGVLERPEAMTFITDNEFIRLPM
                     GVIICHGNGFESLNRIPPGQAITSTSDGRKILNLPLGSRYLIECLVPVKGENPVIDHV
                     SPLSTYMLYGLIFESRFTMKLLARSIAFFLRTRLGTSPDRGHGLLQKARNAIEQISLF
                     TDFDRQMRAQVRNLEEYSTLITGHSHRPMVRRFPGNRTYVNTGSWTRTIRLELDDLGA
                     RNDLTYAIVGYPRKGPPMVNLMKWHGFRQVTEVVTE"
     gene            2200..3765
                     /locus_tag="PKH54_07350"
     CDS             2200..3765
                     /locus_tag="PKH54_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99744.1"
                     /translation="MRKALLPVLLFVLFAGGCRGDGSSILGGFGQLDSCDYAASCAQA
                     IARASSAEDISPELGARLLALKMNALMKFPPVEEEFLSSIGIVQDAGYTNLLPILEKL
                     AAEPEGKVFADQAAHVVEFMKEPVCTRFMELEAVQKGAGPFAASAAIARISSLIQVLS
                     AVTPENSAWVAGAIRQVVGCSLSDRAEGGAVMLAARTMLGEAMAACPAGDSGDLVLRT
                     VCEQGRRLLAEVSIPLPMVESRSGELAGALLPIGRSIGISFNPPWMIVLSAGRLMLVD
                     QAVLTPDGMQVPQFQPQQLLDLRQPHRQEDIKTALHQALKTRKPWTRPGDDRSTYIFL
                     VVDRSTTFADLAEVAGGFLAESESVPLVAVLPAGGSLALWQPLNFFISSRPLYTLDGR
                     MGTFIDVRADGKPGPNAVFELTPFGIKAGGRAVEISRSGDGSSQSGPDLRDVNRLAAE
                     LVAGGAAHAATLKVSQAVTVEHLLSVMQSLSYRMPETAFASAGAYLSSQEVRKRLGEY
                     DYLTAWLVLDRNW"
     gene            3802..4836
                     /locus_tag="PKH54_07355"
     CDS             3802..4836
                     /locus_tag="PKH54_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOC99745.1"
                     /translation="MEGIVVDFRSDTLTRPTPAMMQAISSAVLGDDVYGEDPTVNELE
                     AIAAQLSGKEAAIYTVTGTMANSLAIRLQTEPGDELLMHGLCHPFHHEGGSPASVSGV
                     TVRPLSGEYGLVTADILKASLRPDNRHMSRQKMFWMENTHNAGGGAVLPFDVMRQTAG
                     IARDAGLKVHIDGARIFNASVASGIPVSEYAGLADTMSFCLSKGLGAPVGSLLCGSAE
                     LVHKARRFRHMLGGGWRQAGILAAAGIYALRNNVSRLSIDHANARRLAEAIDAGGHLK
                     VTAPVHTNMVMFAPRDPSVSTADLVTKLAGLGIKVGRSTCGSCRAVTHLDIGPEEMAR
                     AAEVFSSFRM"
     gene            4873..>5220
                     /locus_tag="PKH54_07360"
     CDS             4873..>5220
                     /locus_tag="PKH54_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOC99746.1"
                     /translation="MIPAHVEQIVASGGAAGGVVVVGASASREKYGNMIVRRLLENGF
                     KVYPINPKEKQIEGVDAFQSPSDVDENVAMVSFVTPPPVTLHVLQGMDPSRFKVVWFQ
                     DGSFDEHVIRFAEG"
BASE COUNT          971 a         1482 c         1646 g         1121 t
ORIGIN      
        1 atagaagtca cggacttcac agtcaacccg ggatgactcg ccggccacga tcgattgagg
       61 gtcaagcttc gtgtagattt tacgggcgtt gttcggctcg accgtcacct ggaccggcgt
      121 cgaatccagc atcgtcgtgt tttcaatgcc gcaggcgata tcgaaggtcc cgaccgcgat
      181 gaactggacg tcaagccccg atgtcttgac gccgttctgg ggcgtcacag cggggaaaaa
      241 ccccgaagta atgggattgt cccagcgatc cttgccggtg cacgtcacat gaacccaggc
      301 gccggccttg accagcgtcg gtgttgcctc ggtatcgatt tgaacggcca ttccgggatc
      361 cacgcttatg cgttcgggct gaagatcgat catcccggcg tcgggcgcgt aacaggtcac
      421 gttgaaagag ccggtcaccg tgaacgtaag ccttccgtct tcaagctcta tgccttccgg
      481 aagttccatc gtgtccggat cgagtttcgt gatttcagga ttatcgcctt cctggggttc
      541 gccctcctgt ccgaaatcga tggtcgcttt cttcggatcc cacacaagga ttctggcaag
      601 gccaggttcc catccgcttc cgatgcaatc gaccttcacc ggcagcccgg cctgggtcat
      661 gttcgaggcg acgacggtgt cgatcgagtc gaccggaatg ccggaatcca gggaatcggc
      721 gccgatatcg ccggaaatcg tatcgggcga ggtattcgga ccaccgccgc agccggctgc
      781 cgaagcaagc gtcatcatcg cagcagccag gaatatcgac agccttcgtg actttgtcat
      841 tgggggacct ccggtttcat cgaaacccag tcaggggaca gaaaattcta ggtatatatt
      901 gaagatcatg caaggaatgg ttcattcggt gacgacttcc gtaacctgcc ggaatccgtg
      961 ccatttcatc aggttcacca ttggcggccc tttgcggggg tatccgacga tcgcgtacgt
     1021 caggtcgttc ctggcaccca ggtcgtccag ttccagtcgg atggttcttg tccagctccc
     1081 ggtgttcaca taggtgcggt tcccggggaa gcggcgaacc atcggtctgt ggctgtggcc
     1141 ggttatcaac gtggaatatt cctccaggtt ccggacctgg gcccgcatct ggcggtcgaa
     1201 gtcggtgaaa aggctgatct gctcgattgc gttcctggct ttctgcagca gcccgtggcc
     1261 tcgatccggc gacgttccca gcctggtccg caggaaaaac gcgattgagc gggcgagcag
     1321 cttcatcgtg aacctgcttt cgaaaatgag tccatacagc atgtaagttg aaagcgggga
     1381 cacgtgatcg attaccgggt tttcaccctt gacaggcacc aggcattcga tgaggtagcg
     1441 gcttccaaga ggcaggttca gtatcttcct gccgtcggag gtcgaagtga ttgcctgtcc
     1501 cggcggtatc ctgttgagcg attcaaaccc gttgccatga cagatgataa cgcccatggg
     1561 caggcggatg aattcattgt cagtgatgaa tgtcatcgct tccggccgtt ccagaactcc
     1621 aaggcgggca cggaggactt tctggaccag gggccacgcc acgtcgatgt cgtgattgcc
     1681 cgcgttgtag gtgatggtgt gtccaggcac ccccgcgaac gcggcaagcg cgtcgaaaac
     1741 ggccggatgc cccttgatgc agcgggcgac cttccaggcg gaagcctctt ccgttatttc
     1801 ggtcgtgtga tgtccctcga aagggatttt tatggtgtcc aggaagtcgc cgttaataaa
     1861 cagttccacc ggcacgtccg cccattcgcc ggtgctgtaa tgccgaatca ggtcggacag
     1921 ttcccggtcg tggtgaaagt cctcgaaagg gttgacgatt cccggtgcgt ggccagcccc
     1981 cagatgggtg tccgagaaga tgaccttgat ttccgagagg cctgccggag tcattttcgc
     2041 ttccacaagg gattgacctg cctgaccgtc agtcatgaca ctgtaacggt tgatgaacaa
     2101 tggtatcatc aagcgcgttc cgttgaaacg gattgggggc cgtcgtgccg ccggaccgcg
     2161 gacggaaccc gaactgttca tcattccagc gggggaaaga tgcgaaaggc gttgcttccg
     2221 gtcttgttgt tcgtgttgtt cgcggggggc tgccgcgggg acggatcgtc gattcttgga
     2281 gggttcggcc aactcgattc ctgcgattat gctgcttcat gcgcccaggc catcgcacgc
     2341 gcctcgtcgg cggaggatat ttcgccggag ctcggggcca ggctcctggc gctgaagatg
     2401 aatgccctga tgaaattccc gccggtcgag gaagaattcc tttcttcgat aggtatcgtt
     2461 caggatgcgg gatacacgaa tctgttgccg atccttgaga agctggccgc cgagccagaa
     2521 gggaaggtgt ttgcggatca ggcagcgcac gtggttgagt tcatgaagga gccggtctgc
     2581 acgcgcttca tggaactgga ggcggtacag aaaggcgccg gcccgttcgc cgcatcggcc
     2641 gcgattgcca gaatctcatc tcttatccag gttctctcgg ccgtgacccc ggaaaacagc
     2701 gcgtgggtgg ccggcgccat acgccaggtg gttggatgtt cgctttcgga cagggcggaa
     2761 ggcggcgcgg tcatgctggc cgcccggacg atgcttggcg aggcgatggc ggcctgtccg
     2821 gccggcgatt ccggcgacct tgtgcttcga accgtgtgtg aacaggggcg caggcttctg
     2881 gccgaggtgt cgattccgct tccaatggtg gaaagcagat ccggtgaact tgcgggggct
     2941 ctgctgccaa tcggccgatc gatcggcatt tcgttcaatc cgccctggat gattgtcctt
     3001 tcggcgggtc gtctgatgct ggtcgatcag gcggtgctta cccccgatgg catgcaggtt
     3061 ccgcagtttc agccgcagca gctccttgat cttcgccagc cgcacaggca ggaagacatc
     3121 aagaccgcgc tccaccaggc tttgaagacc aggaagccgt ggaccaggcc gggtgatgat
     3181 cgatcgacat acatattcct tgtcgttgac cgcagcacga ctttcgccga cctggccgag
     3241 gttgccggcg ggttcctcgc ggaaagcgag tcggtacccc tggtcgccgt ccttccggcg
     3301 gggggctcac tggcactgtg gcagcccctg aacttcttca tcagttcaag gccgctttat
     3361 acgctggacg gcaggatggg cactttcatc gacgtcaggg ctgatggcaa gccggggccc
     3421 aacgcggtgt ttgagctgac gccatttggc atcaaggccg gcggcagggc ggtcgaaatt
     3481 tcccgttccg gcgatggatc ctcgcagtca ggaccggatc tgagggacgt caacaggctt
     3541 gcggccgaac tggtggccgg tggagcggct catgcggcga cgttgaaagt ctcgcaggcg
     3601 gtcaccgtgg aacacctgct ttcagttatg cagtcgctct cgtaccgcat gcccgaaacc
     3661 gcctttgcgt ccgccggggc ctacctttca tcgcaggaag tccgcaagcg gcttggtgaa
     3721 tacgactacc tgacggcctg gctcgtgctt gatcgcaact ggtagccacg ttgccgaatc
     3781 ttttcggagg cattgacaca gatggaagga atcgttgttg attttcgttc agacaccctg
     3841 acgcggccga cgccggccat gatgcaggca atttcgtccg ccgtccttgg cgacgacgtc
     3901 tatggggaag acccgaccgt aaatgaactg gaggcgattg ccgcgcagtt gagcggcaag
     3961 gaagccgcga tttacacggt gacgggcacc atggccaatt cgctggcaat caggcttcag
     4021 accgagcccg gcgatgaact tctgatgcac gggctgtgtc atccgttcca ccacgagggc
     4081 gggtcgcccg cttcggtttc gggcgtgacg gtcaggccgc tttccggcga gtacggcctg
     4141 gtaaccgccg acatccttaa ggcgtccctt aggccagata accgtcacat gagtcgtcag
     4201 aagatgttct ggatggagaa cacgcataac gccggcggcg gcgcggtgct tccgttcgac
     4261 gtgatgcgcc agaccgcggg aatcgcacgc gacgccggcc tgaaggtcca tatcgacggg
     4321 gccaggattt ttaacgcatc ggtcgcatcc gggattccgg tcagcgaata cgccggcctt
     4381 gccgatacga tgagtttctg cctgtcgaag ggactcgggg cgccggtagg aagcctgctg
     4441 tgcggatcgg ctgaactggt tcacaaggcc cgcaggttca ggcacatgct tggcggcggc
     4501 tggaggcagg ccgggattct cgcggccgcg gggatttatg cccttcgaaa caatgtttcc
     4561 aggctgtcca tcgatcacgc caatgccagg cgccttgcgg aagcgatcga tgccggcggt
     4621 cacctgaagg tcaccgcccc ggttcacacc aacatggtga tgttcgcccc gcgggatcct
     4681 tcggtttcaa cggcggatct cgtcacgaag cttgcgggac tcggcatcaa ggttggccgg
     4741 agcacttgcg gatcctgtcg ggcggtcaca catcttgaca tcggccccga agaaatggct
     4801 cgggcggccg aggtattttc aagtttcagg atgtgatcgg gacatcccgt tcgggatgtc
     4861 gggaggtgcc ccatgattcc ggcgcacgtt gaacagattg ttgcctccgg cggcgcggcc
     4921 ggcggcgttg tcgtggtcgg cgcctccgcc agccgcgaga agtatgggaa tatgattgtc
     4981 aggaggctgc ttgaaaacgg attcaaggtc tatccgatca atccgaagga gaagcagatc
     5041 gagggcgtcg atgccttcca gagtccgtcc gatgtggatg aaaacgtcgc gatggtcagt
     5101 ttcgtgacgc cacctcccgt cacgctgcat gtccttcagg gtatggatcc ttcccgcttc
     5161 aaggtggttt ggttccagga cggctccttt gacgagcatg tgatccggtt cgcggaaggc
//
LOCUS       DAOPPA010000208         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17368_length_5210_cov_3.465761, whole genome shotgun sequence.
ACCESSION   DAOPPA010000208 DAOPPA010000000
VERSION     DAOPPA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17368_length_5210_cov_3.465761"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKH54_07365"
     CDS             complement(<1..259)
                     /locus_tag="PKH54_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99747.1"
                     /translation="MNGSDRTAILIPIHIDLGIVQIPNHNAGLHLQIHGSVTVEQCDL
                     EAENDMGVRVNGTSGRQSWDVEDRRNPSQKFSGLTATMSGTG"
     gene            complement(256..3339)
                     /locus_tag="PKH54_07370"
     CDS             complement(256..3339)
                     /locus_tag="PKH54_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOC99748.1"
                     /translation="MEDTAFRNLVEDVRFRTDIVDVIGGDLNLTPCGSILKGRSPFNK
                     DDTPSLVVWPETQTWHDFSGGGSNGGDCFEYVQRRDNCAFIPPPQRLADRAGITVPER
                     TDVSPETAATSEDRRRLEAMLTMAADYYHDALPNDVRQDWLHNRYGLTDETIDRFRIG
                     WADGGLFTFMTGDLGVTREEALSTGLFVVLDDGAVVDFFQGRIVFPYWKRGRVVYFTG
                     RRTDRTPEKPWEDAKYRKLPGQSEKRPYISLLLSNDHFFNEDAARGADLILITEGITD
                     CIAGAQAGLACISPATTRFRAKDHEKLLAITAKASKIVICNDSEISNAGETGAVETAA
                     ILYHAGRNVRLATIPRAAETDKIDVNDLVRTQGKDGLRRVVEAAISLPRFLVEKVPAD
                     TPKEDLVTALQPAIELVKDASELDRDVTSDLLQKRFGLRKASVRELLDSNKDNRHENG
                     PRTTPAISTGPRKGEVFEHGAHYCGIGRGGETVILSSFTMKATRRIVVDGNEILETTI
                     TAENGRIFEKVLLRREAWLSKPAFLRSLPSVDLQWTGSDENVQGVLRLVASQDAPVSR
                     GTFTLGYHDTDAGPRWVTAKAVVAPEEIPPDAEPILYIPSGSHLHERIAFPTDVSTDE
                     EKALASVALSAILQLNRPEVVLPILGWFFATPLKPRLMGVFGHFPFLMVWGTQGSGKS
                     SIISNVFWPLFGFKGTEPYSATETEFALIKLMSCTNSVPVFVDEYKPFDMPKHKLDRL
                     HRLLRQIYDGEIEERGRPDQQVNAYRLSAPVVLAGETRPTEPALVERMLPVNPDKNAL
                     VTHPEYATAMATLRSLDLTKLTAGILRFLLKTDVDARVSMAREIADGLLEGRTIPPRV
                     KDSIVIMIFGLRVFKEYAESRGVDIGVLQVDKAVTGILDDLLEAGGNSVKSVLDRFVE
                     ELHVMAVQGRILSGREYIVSGNMLNIHLGACHAAYTEHCRRIGYTGEVPDRMALRRQF
                     NESLVSGGYVTAVDELVCFNGRQDRRRCVVIDLEKARNTLAIDDFPARSTGMVAS"
     gene            complement(3362..3604)
                     /locus_tag="PKH54_07375"
     CDS             complement(3362..3604)
                     /locus_tag="PKH54_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99749.1"
                     /translation="MDRQERMMLADVITEVALSLVGVFDAFNADAEMVDAVSNALRKV
                     FAKYQEAGDSTAKNDATGTLKKLIDEMEASSGRVRG"
     gene            complement(3632..4486)
                     /locus_tag="PKH54_07380"
     CDS             complement(3632..4486)
                     /locus_tag="PKH54_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99750.1"
                     /translation="MPASKPSNVSGPCNLRTIQRGLRQVVFSPSNVKVFESAQARRPA
                     LREFAGIESVLGTLADRTRCPASRADRIVSALINEYQREHHAFWSSVLTLTFMPMLGN
                     ILRQIDEPILAYEDLSQTLILAFHEVLAELDLSESQRLTILRLRQETRRRVFSAIKEE
                     RRIRGLYRTFDDNGLINLSNEDCEWPDSPIPPSIEDPVIIETAVEMLRGVATRFISDD
                     DIELIACTAIRGESLWKYVDRKFSGSSDPARIRAHARLKRRRTRKIVEIRYLFAKLFV
                     PKTDVSAS"
     gene            complement(4837..>5210)
                     /locus_tag="PKH54_07385"
     CDS             complement(4837..>5210)
                     /locus_tag="PKH54_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99751.1"
                     /translation="VAASIMLMYLTDPTQGFEPWKPKELAELFSGYKFEGLGDIESVK
                     DLKKQIKRSGNSYTYVSRDWVVFPAEPYCCVDVRTFCGGEYYPDENFDPTEDDSPENG
                     PWVKEIDAQTDEPEAERKSCS"
BASE COUNT          957 a         1461 c         1552 g         1240 t
ORIGIN      
        1 cacctgtccc gctcattgtc gcggtaaggc ccgagaactt ttggctcgga ttccggcggt
       61 cttccacgtc ccatgattgc ctccctgaag ttccgttgac cctgactccc atgtcgtttt
      121 cggcctccaa atcgcactgt tcgacggtga cactaccgtg gatttgaaga tgtaatccag
      181 cgttatgatt ggggatttgt acgattccca agtcaatgtg gatggggatc aggatggcgg
      241 tgcggtcgct gccgttcatg acgccaccat gcccgttgat cgcgccggaa agtcgtcgat
      301 ggccagcgta ttcctggctt tctcgaggtc gataactacg cacctgcgcc tgtcctgccg
      361 accgttgaag cagaccagtt cgtcgacggc ggtgacgtat ccccctgaga ctagggattc
      421 attgaactgc cttctgagcg ccatacggtc cggaacctcg ccggtataac cgatccgccg
      481 gcagtgctcg gtgtaagcgg cgtggcaggc gcccaggtga atgttcagca tgtttccgct
      541 tacgatgtat tcacggccac tgaggatacg gccctgcacc gccatgacat gcagttcttc
      601 gacgaagcgg tccagaactg acttcacgga gttgcctcct gcttccagca ggtcgtccag
      661 gattcctgtg acggctttgt cgacctgcaa caccccgatg tcgacacctc ggctttctgc
      721 gtactccttg aacaccctga gcccgaagat catgatgacg atggaatcct tgactcgtgg
      781 tggaatggtg cggccttcca gcagaccgtc agcgatttcg cgggccatgc tgacccgtgc
      841 gtccacgtcg gtcttcagca gaaagcgcag aatcccggca gtcagcttgg tcaggtccag
      901 gctgcgcagg gtagccattg cggtggcgta ttcgggatgg gtgaccagtg cgttcttgtc
      961 gggattaaca ggaagcatac gctcgaccag cgctggttcg gtgggcctgg tttcacctgc
     1021 caggacgaca ggagccgaca gtcggtaggc gttgacctgc tgatcgggac ggccgcgttc
     1081 ttcgatttcg ccgtcgtaaa tctgacgaag cagccggtgc agacggtcga gcttgtgctt
     1141 gggcatgtcg aagggcttgt actcgtccac aaataccggc acggaattcg tgcatgacat
     1201 cagcttgatg agcgcgaact ctgtttctgt ggcggagtat ggctctgtcc ccttgaaccc
     1261 gaacaaaggc cagaacacgt tcgagatgat ggacgacttg ccggacccct gcgtgcccca
     1321 gaccatcagg aaagggaagt gtccgaagac gcccatcagg cggggcttga gcggcgtggc
     1381 gaagaaccat ccgaggatcg gtagcacgac ctcgggacgg ttcagttgca ggatggcaga
     1441 caaggcaacg gatgccagcg ccttttcttc gtcggtgctg acgtcagtcg gaaaggcgat
     1501 gcgttcgtgc aggtgcgacc cagacggaat gtacaggatc ggctcggcgt cgggtgggat
     1561 ttcttcaggc gcaacaacgg ccttggctgt gacccatcga gggccggcat cggtgtcgtg
     1621 ataccccaag gtgaaagtcc ctcgcgaaac gggcgcatcc tggctggcca ccagccgtag
     1681 aactccctgg acgttttcgt cgctgccggt ccactgcagg tcaaccgagg ggaggcttct
     1741 taggaatgct ggctttgaca accacgcttc ccggcgcagc agtacctttt cgaatatgcg
     1801 accgttttcg gcggtgatag tggtttccag aatttcgttg ccgtcgacca cgatacggcg
     1861 ggtcgctttc atagtgaacg acgacagaat gactgtttcc ccgccacgtc caatgccaca
     1921 gtaatgcgcg ccgtgttcaa agacctcgcc cttccgtggt cccgtggata tggcaggggt
     1981 ggtccgcggg ccgttttcat gtctgttgtc cttgtttgag tcgagtagtt cccggacgct
     2041 ggcctttctc agaccgaagc gtttctgcag aaggtcggaa gtgacgtccc ggtcaagttc
     2101 ggaagcgtct ttgacgagtt caatcgcggg ttgcagtgcg gttacgaggt cctctttagg
     2161 ggtgtcggcc ggcacctttt ccaccaggaa ccgtggcagg ctgatggccg cttcaacgac
     2221 ccggcgcagg ccgtctttgc cctgcgtcct gactagatcg ttgacgtcga ttttgtccgt
     2281 ttctgccgcc cttggaatcg tggcgaggcg gacgttgcgg ccggcgtggt agaggatcgc
     2341 ggccgtttcg acggcgccag tttcccctgc gttgctgatt tcggaatcgt tgcagatgac
     2401 gatcttgctg gctttggcgg tgatggcaag gagcttctcg tggtccttcg cgcggaatct
     2461 ggtggtggcc ggcgaaatac aggctaatcc agcctgggcg cccgcgatac agtctgttat
     2521 gccctcagtg atcaggatca ggtctgcgcc cctggccgcg tcctcattga agaaatggtc
     2581 gttggacagc agcagcgata tgtatgggcg tttttctgac tgaccgggca gcttccggta
     2641 tttcgcgtct tcccagggct tttctggtgt ccggtcagta cggcggccgg tgaagtacac
     2701 gacccggccg cgtttccagt atgggaacac tatccgccct tgaaagaagt cgaccacagc
     2761 gccgtcatcc aggaccacga acagacccgt ggacaaggct tcttccctag ttacgccgag
     2821 gtcgccggtc atgaacgtga acaggccgcc gtcagcccag ccgatcctga atcggtcgat
     2881 ggtttcgtcg gtcagtccgt atcggttgtg cagccagtcc tggcggacat cattcggcag
     2941 ggcgtcgtgg tagtagtcgg ccgccatagt cagcatggcc tcgaggcgac ggcgatcttc
     3001 gcttgtggcg gccgtttcgg gactgacgtc cgtgcgttcc gggacggtta ttccggcgcg
     3061 gtcggccagc ctttgcggtg gggggatgaa ggcgcagttg tcccggcgct ggacgtattc
     3121 aaagcagtcg cctccgttgg agccgccacc ggagaaatcg tgccatgtct gggtttccgg
     3181 ccatactacc agtgatggtg tgtcgtcctt gttgaacggg gaacggccct tcagtatgga
     3241 accgcacggg gtcagattca ggtcgccgcc gatgacgtcg acgatgtccg tcctgaacct
     3301 cacgtcctcg accagatttc ggaatgcggt gtcttccata tccaacccca tgcggtcctg
     3361 atcagcctcg aacgcgtccc gacgaagctt ccatttcgtc gatgagcttt ttcagggttc
     3421 cggttgcgtc atttttcgct gttgaatcgc cggcttcctg gtacttcgcg aagactttac
     3481 gcaaggcgtt tgaaacagcg tcgaccatct ccgcgtccgc gttgaatgca tcgaagacgc
     3541 cgaccaggct gagcgcgact tctgtaatca cgtctgccaa catcattctt tcttggcggt
     3601 ccatggagcc tccttgttgc ccctacctcc atcaagaggc ggacacgtcc gttttgggga
     3661 caaacagttt tgcgaacagg tatctgatct caacgatttt tctggtccgg cggcgcttaa
     3721 gtcgtgcgtg agctcgaatt ctggctggat ctgatgaccc ggaaaatttc ctgtccacgt
     3781 acttccacag ggattcgccc ctaatggctg tgcatgcgat tagttcgatg tcgtcgtcgg
     3841 atatgaatct ggtggcgaca ccgcggagca tctcgacggc ggtttcgatg atcacagggt
     3901 cttcaattga gggtggaatt ggcgaatcgg gccattcgca gtcctcattc gacaggttta
     3961 tcaggccgtt gtcgtcaaat gtcctataca gacccctgat tcgtcgctct tccttgatcg
     4021 cggaaaatac ccggcgtctg gtttcctggc gtagacggag gatcgtcagt cgttggcttt
     4081 ctgacaggtc gagttccgcc aggacttcgt gaaatgccag aatcagagtc tgcgataggt
     4141 cttcgtatgc cagaattggt tcgtcgatct ggcggaggat gttcccgagc atcggcatga
     4201 acgtcagcgt caggacactg gaccagaatg cgtggtgctc gcgctggtat tcgttgatga
     4261 gcgcgctcac aatgcggtcg gctcggcttg cagggcatct cgtccggtcg gcaagtgtcc
     4321 cgaggacgct ttcaatcccg gcgaattcgc gaagcgctgg tcgtcgcgct tgagctgatt
     4381 cgaaaacctt tacattgctg ggggaaaata ctacctgccg taatccgcgc tggatggtcc
     4441 tgaggttgca tggcccggac acgttcgatg gtttcgatgc tggcatcatc ttctcctggc
     4501 gagttcccga tggggggaaa ctcacgttta acgggctccg gacagttggc cttgctgcgt
     4561 gccccgtctg atcgaagtct gcaactggtc ggaccgcgag gtgaaaacag cccggtcgac
     4621 tagtctggcc gccctgccgt tcgacaccgt cggaccgact caaaccagcc tagctcgaat
     4681 ggccagatct ctggtttgcg acacttccgg cagcaaacca gataggtcgt tggagcgatg
     4741 atgtatgatt tggggaaatg gtaatagata cgctaagtta agaattattt ggcgggggtt
     4801 taccccccct ctcgatgggg ctcaggttaa ccccctttag gagcaacttt tcctttcagc
     4861 ctccggttcg tctgtttgtg catcgatctc tttcacccac ggtccgttct ccggggagtc
     4921 atcctcggtg gggtcaaagt tctcatcggg gtagtactca ccgccgcaaa aggtccttac
     4981 atcgacgcaa cagtatggtt cggccggaaa aactacccag tctctggaaa cgtaggtgta
     5041 tgagttgccg gaccgcttga tctgcttttt caggtctttt acgctctcaa tgtcgccaag
     5101 accttcaaac ttgtatccgc tgaaaagttc cgcaagctcc ttcggtttcc agggctcaaa
     5161 tccttgagtt ggatcggtaa ggtacatcag catgatcgag gctgcgaccc
//
LOCUS       DAOPPA010000209         5189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17451_length_5189_cov_3.107908, whole genome shotgun sequence.
ACCESSION   DAOPPA010000209 DAOPPA010000000
VERSION     DAOPPA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17451_length_5189_cov_3.107908"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PKH54_07390"
     CDS             complement(<1..984)
                     /locus_tag="PKH54_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99752.1"
                     /translation="MRIRILLAAATIGAFMPVSCTTLAPTDVQDPTDTVTLDEGSTDA
                     AFDVEQDTNDVSDAAADVSNDTARDIPDIPETFPDIPPIECVSDLACERAWPDLPECW
                     HAFCDKWLGICIVAPQVEGSACDSGNKCIKDGSCIEGQCVGPALDCDDANPCTDDSCD
                     PEDGCLHTTNKAACEDGNPCTSNDSCINGNCRPGPNSCDCQGDQECAEYEDGNPCNGL
                     IRCLGQKCVLNKSNVPACEAMQDYCLANMCNPETGLCEPTPVNEGSRCDDGNECTVND
                     VCRGGICAGDAGWCDDGNDCTVDSCVPGEGCIHEFNSAPCDDGNLCTRDDAC"
     gene            1165..2622
                     /locus_tag="PKH54_07395"
     CDS             1165..2622
                     /locus_tag="PKH54_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99753.1"
                     /translation="MTWVVMASYVVLGVLAIHVAIANHAGGLATSIMDGVDSLGLSDI
                     WELQANKRESRIWMQLMMWGFHPSHGLFSVFFGDSWPTLFLDLLYSMLAVLAGGFIVF
                     RYSRQVLSDSRTSFLLVFLYFLLPVTFANTVYWYGQPVFVMVMGALYLMRRGNHLPAA
                     LLVIWAHGCHPMAAPVFLTLSWASFRMDLKGRPPILPGLDRRDAVAARSWRFSLVMFA
                     ALSAWTGFLVLMSRLADSSSTRGLLWDFFSGKFDPSALPFNFSQTVFFFLPMLFLPFA
                     SLVWLPSLVVFTGYMLFGSQGVVTGFVLPAAGFSMLAVAWSMIRLSGKTRAWLAGGAV
                     VAALLVNLLVPWPAIFPMQPEPLTGGIFAHHSWSVQAGESAINSMIYEQIPAGTDRCL
                     TSWQIGPVMARQCRNVTTLSFPFRRQEYALGDFLDSGDRGRIADGWWNFILVDTRREA
                     NSERLSELFHRIEYSGRWEVAGRTAEAVLYRRVDP"
     gene            complement(2601..3575)
                     /locus_tag="PKH54_07400"
     CDS             complement(2601..3575)
                     /locus_tag="PKH54_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99754.1"
                     /translation="MNQQNQRNAGWLREYLPWIAGAALVLLVLAEAVLRIAVAPTWPP
                     VKTDFDHGDRNCFRPVPDSSVAYRRYGLAGEPVVHSVNRLGFRGHIPDRANDFVVAVV
                     GDDLVYGVGLPESETIPAVLERRLRTRFPDASIRVANLGWPGFNLEEQSLEYERLARE
                     LKPDITILLLSDRYSSPSACSNAALPARISLRRFSAVWNQLESALERATFNTATLFSV
                     PAREYPIKVILERLSGHGTDLVYARTFATPPVFQEDGRTMGFDDLAESAGFQILDMTD
                     RFDSSMPGRPPLKDDADLSPSTVKAVVDATAGFVEPIIRNRLQGSTRL"
     gene            complement(3598..5061)
                     /locus_tag="PKH54_07405"
     CDS             complement(3598..5061)
                     /locus_tag="PKH54_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC99755.1"
                     /translation="MNRFPGALRLAVTVSVISTTLWLFSGCGADGTQMDTFGSDVFDE
                     VGPDVIQADEGGDGEGRDANQPEDGGADTGHADTTHGPDAEKDTGSGSDLSDPDQGGT
                     DLSDPDHGATDGQVVDEGQPSDNGQPSDDGQADATVISCQPESQLPGIKVTVYRDLKD
                     STTKPANNQSKTDQIQTFSKFDEPWAGIPVNIIDPFDLSGMTCDEGWYRFDPDDGEVF
                     SDGVRILDIGLPECRVTTHNVSRNTPAALAAGRWKMIVMGDSIPVLGTTSGQFFHDNV
                     AADLEPFGDILVFNHAYSGAVTSGWLPTTSNFKNKIKPALADTDVVFVSLGGNDIMHY
                     AYNNTNKPLDQLMDGITQTISSVQQNLRTIFDAIWAENPDVEIVWLIYPNYARSNEWA
                     SMVGEQYAPMIESYFEEQMYGIVSWAAGIEGLLLVDLFSRMNKIDLDKSLYDELHYNQ
                     YGHRILGNELLKILGGVKIEDGVPTTGGERMIGVICD"
BASE COUNT          892 a         1545 c         1543 g         1209 t
ORIGIN      
        1 gcacgcatcg tccctggtgc aaaggtttcc atcgtcgcac ggggcgctgt tgaattcgtg
       61 gatgcagccc tcgccgggga cgcacgaatc gaccgtgcaa tcgttgccgt cgtcgcacca
      121 cccggcatca cccgcgcaga tgccgccacg gcacacgtcg ttgaccgtgc attcgttgcc
      181 gtcgtcgcac cgggatccct cgtttaccgg agtcggttca caaaggccgg tttccggatt
      241 gcacatgttc gccaggcagt aatcctgcat cgcctcgcag gcaggcacgt tcgacttgtt
      301 aagcacgcat ttctggccaa ggcagcgaat cagcccgtta catgggttgc cgtcctcgta
      361 ttcggcgcat tcctggtcgc cctggcagtc gcatgaattc ggtcccggcc tgcagttgcc
      421 gttgatgcac gagtcgttcg acgtgcacgg attgccgtct tcgcaggccg ccttgttcgt
      481 cgtgtgcagg cagccgtctt ccgggtcgca tgagtcgtcg gtgcacggat tggcgtcgtc
      541 gcagtccagt gccgggccga cgcactggcc ttcaatgcac gagccgtcct taatgcactt
      601 gttgccggaa tcacaggcgg aaccttcgac ttgcggtgcc acgatgcaga ttccaagcca
      661 cttgtcgcag aacgcgtgcc agcattccgg cagatccggc caggcccgtt cgcacgcaag
      721 gtcggagacg cactctatcg ggggaatgtc cgggaatgtt tcgggaatgt cgggaatatc
      781 ccgtgccgtg tcgttggaaa cgtcggcagc cgcgtccgac acgtcatttg tgtcctgttc
      841 aacatcgaat gccgcgtcgg tcgaaccttc atccagcgtg acggtgtccg tgggatcctg
      901 gacgtcggtc ggggcaagag tggtgcatga aaccggcatg aacgcgccga ttgtggcggc
      961 tgccagcagt attcgtatgc gcatcgacat ctccggtgtt acctttctga ggcctttggt
     1021 gccggccata ttctatcagt cgccgcgcat actcgcaaag accgtttccg gcggatatac
     1081 ttggacgtcc ttcagcggat tcaacgcgtg cgccggccct tcaaatcaac cgattcggtg
     1141 tgccgactcg actgctggca tttcatgaca tgggttgtga tggcgtcgta cgtggttctt
     1201 ggcgtgcttg caatccacgt cgccattgcc aaccacgccg gcggcctggc cacatccatc
     1261 atggacggag tcgattcgct ggggctttcc gacatctggg aacttcaggc caacaaacgg
     1321 gaatcccgca tctggatgca gttgatgatg tggggattcc atccttccca cggcctgttc
     1381 agcgttttct tcggcgattc gtggccgacg ctctttctgg atctgctgta ctcgatgctg
     1441 gccgtgctgg cggggggatt catcgttttc cggtattcgc gccaggtcct gtcggactcc
     1501 cggacatcgt ttctgctggt ctttctgtac ttcctgcttc cagtcacttt cgcgaacacg
     1561 gtctattggt atggccagcc ggttttcgtc atggtcatgg gcgcgttgta cctgatgcga
     1621 cggggcaacc acctcccggc ggcccttctg gtcatatggg cgcatgggtg ccatccgatg
     1681 gccgccccgg tctttctgac tttgtcctgg gcatctttca ggatggatct gaagggcagg
     1741 ccgccgatac tgccggggct tgaccggcgg gatgcggtcg ccgcgcgatc ctggcggttt
     1801 tcgcttgtca tgttcgccgc tctttccgca tggaccggct tcctggtgct tatgtccagg
     1861 ctggcggatt cctcctcgac acgtggcctt ctctgggact ttttcagtgg caagttcgat
     1921 ccttcggctt tgccgtttaa tttctcccag accgttttct tctttcttcc gatgcttttc
     1981 ctgccattcg cctcactggt ctggcttcct tccctggtgg tatttaccgg gtacatgttg
     2041 ttcggatccc agggggtggt gacaggcttc gttcttccgg ccgcgggttt ttcgatgctg
     2101 gccgtcgcat ggtcgatgat ccgcctgtcc ggaaagacca gggcctggct tgccgggggc
     2161 gccgtcgttg ccgcactgct ggtcaatctt ctcgtgccgt ggccggccat tttcccgatg
     2221 cagcccgaac cgttgaccgg cgggattttt gcccaccatt catggtcggt tcaggcgggt
     2281 gagtcggcaa tcaattcgat gatatacgag cagattccgg ccggtaccga cagatgcctg
     2341 acctcctggc agattggccc ggtgatggcc aggcagtgcc ggaatgtcac cacgctttcg
     2401 tttcctttcc ggcgccagga gtacgccctt ggcgacttcc tcgacagcgg ggatcggggc
     2461 aggattgccg acggctggtg gaatttcatc ctggtcgata ccaggcgcga ggccaattcg
     2521 gaacgtcttt ccgaactgtt tcaccgtatc gagtattccg ggcgctggga ggtggccgga
     2581 cgaacggccg aagcggttct ttacaggcgc gtcgatccct gaagccggtt tctgattatc
     2641 ggctcgacga atccggccgt tgcatcgaca acggccttga ccgtcgatgg cgaaagatcg
     2701 gcgtcgtcct tcagcggcgg tcgtccgggc atcgacgaat cgaatctgtc cgtcatgtcc
     2761 aggatttgga aacccgccga ttctgccaaa tcgtcaaacc ccatcgtcct tccgtcttcc
     2821 tgaaaaaccg ggggcgtcgc gaaagtcctg gcatacacca gatccgtgcc gtggcccgag
     2881 agtctttcaa ggattacctt gatggggtat tccctggccg gcaccgaaaa caacgttgcg
     2941 gtattgaagg tggcacgttc gagcgccgat tcaagctggt tccagacggc cgaaaaacgc
     3001 ctgagcgaga ttcttgcggg cagtgcggca ttggagcacg cggatggcga ggaataccga
     3061 tccgaaagca acaggattgt tatgtctggt ttgagttccc gtgccagcct ttcgtattca
     3121 agcgactgtt cttcgaggtt gaatcccggc catcccaggt tcgcgacgcg gatcgatgcg
     3181 tcgggaaacc gcgtgcgaag ccgtctttcc agcacggccg ggatggtttc gctttccggc
     3241 agtccgacgc catacacaag gtcatccccg acgacggcca ccacgaaatc gttcgccctg
     3301 tccggaatat gaccacgaaa gccaaggcga ttgaccgaat ggacgacggg ctctccggca
     3361 aggccgtaac gccggtatgc gacgcttgaa tcgggaaccg gcctgaagca gtttcggtcg
     3421 ccgtggtcga agtcggtttt caccggcggc cacgtcggcg ccaccgcgat tctcagcacc
     3481 gcttcggcaa ggacgagcag cacgagagcg gccccggcaa tccaggggag atattcgcgc
     3541 agccacccgg cgttccgttg attctgctgg ttcattccgg ccccgcggcg ctcgtcatca
     3601 gtcgcatatc acgccgatca tgcgttcgcc acccgtcgtc gggactccgt cctcgatttt
     3661 gacgccgcca aggattttca gcagttcgtt gccaaggatc ctgtggccgt actgattgta
     3721 atgaagttca tcgtacaggc tcttatcaag gtcgatcttg ttcatgcggg aaaacagatc
     3781 gaccagcagc agcccctcga tgccggcggc ccaggaaacg atgccgtaca tctgttcctc
     3841 gaaatacgat tcgatcatcg gtgcgtactg ttccccgacc atggatgccc actcattgga
     3901 tctggcgtag ttcggataga tcagccagac gatttcaacg tcggggtttt cggcccagat
     3961 cgcgtcgaaa atcgttcgca ggttctgctg gaccgatgaa atcgtctggg tgatgccatc
     4021 catcaactgg tcaagcggct tgttcgtgtt gttgtacgcg taatgcatta tgtcgttgcc
     4081 gccaagcgac acgaaaacaa cgtcggtgtc ggcaagcgcc ggcttgatct tgttcttgaa
     4141 attgcttgtg gtcggcagcc atccggaggt gacagccccc gagtatgcgt ggttgaacac
     4201 caggatatcc ccgaacggtt caaggtccgc tgcaacgttg tcgtggaaga actgaccgct
     4261 tgtggttcca agaaccggga tgctgtcccc catcacgatc atcttccagc ggccggccgc
     4321 cagggcggcc ggggtgttcc tggaaacgtt gtgcgtggtc acgcggcatt ccggcaggcc
     4381 gatgtcaaga atccggacgc cgtcggaaaa gacctcgcca tcatccggat caaacctgta
     4441 ccagccttca tcgcaggtca ttccagacag gtcgaacgga tcgatgatgt tgacgggaat
     4501 tcccgcccac ggttcgtcga atttgctgaa ggtctgtatt tggtcggttt tcgactggtt
     4561 gttggccggt ttggtggtcg agtccttcaa gtctcgatag accgtcacct tgatgccggg
     4621 aagctgcgat tcgggctggc aggagatcac cgttgcgtcg gcctgcccgt cgtccgaagg
     4681 ctgcccgttg tcggaaggct gtccctcgtc aaccacctga ccgtcggtgg cgccgtggtc
     4741 cgggtcggac aggtcggtgc cgccctggtc cgggtcagac aggtcgctgc cgcttccggt
     4801 gtccttttcg gcatcggggc cgtgtgtcgt gtcggcgtgg ccggtatcgg cgccgccatc
     4861 ttccggctgg ttggcatcgc gcccttcgcc atcaccgcct tcgtcggcct gtattacgtc
     4921 cggccccacc tcgtcgaaaa catccgaacc gaaggtgtcc atttgcgtgc cgtcggcgcc
     4981 gcatccggaa aaaagccaca gtgtcgtgga aatgacggaa acggtcacgg caagccgcaa
     5041 tgcgcccggg aagcgattca tcttcacctt gaaaccccga attgggacgc catattctag
     5101 acggatattc ccctgatatc aacgggactt gcaacgctcg atccgaccat ttgaatggtt
     5161 atatgaaaga cagggacgat ttcctggcg
//
LOCUS       DAOPPA010000210         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17506_length_5180_cov_3.299122, whole genome shotgun sequence.
ACCESSION   DAOPPA010000210 DAOPPA010000000
VERSION     DAOPPA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17506_length_5180_cov_3.299122"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKH54_07410"
     CDS             <1..336
                     /locus_tag="PKH54_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99756.1"
                     /translation="VQIGEDGSGCRKHGVSIWTETVKALVPVEDSKGSTTSAVVGKRL
                     HDYWRLSVGGGSEGSIINYSPDQIASLDSDRCFAVRNRLYKASQRIEQTLQMQKKNQY
                     IWLNFQNIP"
     gene            371..2044
                     /locus_tag="PKH54_07415"
     CDS             371..2044
                     /locus_tag="PKH54_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC99757.1"
                     /translation="MSESNRLFARWSALKKEVTVKDGCGMRVLDLGPGAIPRGRLVCV
                     LGPSGCGKTSLLSILGLVDHEFEGDLGVELGGRFQPIGQWRGRKRQRESARLRRHIGF
                     VFQEMRLRAGASAVENVMDPMLYLGFGTPRTRYSHAKSMLGLFGLQDRDIERPISCFS
                     GGMQQRTGLARALSIGPDLIIADEPTAHVDDKMANAIYEDLKRRSAERGITVVVATHN
                     EEMADKHADHIIRFKPKFQDEEEVRRAKDLGEWPYDLIVEREAEGIEPADAKGETPKR
                     ASTLGSFKDMAVVALGEGYPLVRVTGRGIRALGWGWAAALVNRLRKKPSVRRERIAPH
                     RYLPLLVSIVTFGLLAGMGFSLYGIKSAILAYQQSKLESLESLRRVRVFDPGDLEGGA
                     IRLDVADVEKFAAEHGVKITESVPVHDLGGEALIPSEMAHLASEGQWAPVQSSALLDP
                     SSIDDRERNANRIFFTILGTFRTSHRPGIRACRPSRAGSRQRTMLHRPSGFRGTSGNG
                     FTAISRRFPSREIRSASCSWASPAWNAPRKPRVMSSRPRCACVSISAAT"
     gene            1951..2835
                     /locus_tag="PKH54_07420"
     CDS             1951..2835
                     /locus_tag="PKH54_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC99758.1"
                     /translation="MGIARMERTPEAQGDVIPPAVCVRFNIGGYLNPPRLPSGFVSEV
                     QAKYYKAVVSKDVVKRILEWQNDPLNPAKALPDAWRCDGSPSIYPDWQYTVKDEVRAP
                     AATQFDFFAPTPSDALKLERILEDYSSDRGLTFGRVSSERGFIQQVSRLIKLAEYVGL
                     VLHVLPIAVGGVILWLVVHEILFRRREDLLLFHVMGAPTINLHVQSLVLSMLVVLPGI
                     LIGYIIGSAGPGLMVGVMSNETLPPDLLEHLAKVGIGWVGLLQTTVAAVICAVFASFT
                     VVKSILSSNPASAFRGMQ"
     gene            2832..4796
                     /locus_tag="PKH54_07425"
     CDS             2832..4796
                     /locus_tag="PKH54_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99759.1"
                     /translation="MKSSNNKVVKTMKCAVNVGTLTVFLLLGLLLAPVVASADGAAEF
                     RLVSGFKPMLQKCPASDACEVTLVKEYGAWMWQASCTAKLGEDVFRRLDLLKRREADV
                     VAIVVSKAKRAVAADILDYIDERRQGHLLDSGRLRLVRLEEVQRHKVDEKLLELCAPA
                     VALPDGMSFPPNTAIVFVGDKGEAPKSNPCKDERWRDGVTVLSNMDDIDQIVGRKLDD
                     LISTKSQIEDIKRHERKACEETGDASAFIELVFDPVVKDSAGDSPNLSASDLGLDNAE
                     IVTQLRKMGVEISSQLSSLFPADVERSAMKREDGKWALNAQKLRSLMALADSRAGEKM
                     LKQASAKLIAQIQSAMTRVERFSSTYSGGKGPDLSIDAMKARNELVTRKYGQVCGGSG
                     ITAECSSARARIDELEARLESFEKEPAPIAASPVAQAAPAKPIDEWSREATASLQTLQ
                     GNVVLCKTRECLDGQAIEYARLNGSIERYQREYGASVAAGKGVDPRIVELENLMASAE
                     ADLGAARGTPASWSKMKIGGVAALSAGGLIMIAAIVPLLMANSEMKKIEDATNGGIVP
                     RAWLSIDGFGDQWWYRINKFDVDDIGAMVTRQRMKAIIGWSVLGAGALAAIAGGVLLG
                     VDAMKEGRNKPTVTVAPVESGAVVGVNMSF"
     gene            4808..>5180
                     /locus_tag="PKH54_07430"
     CDS             4808..>5180
                     /locus_tag="PKH54_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99760.1"
                     /translation="MKHTDSMFDKVAAAACIVVLGAVGCVAEQFECDDNKPCSESGEV
                     CVYYLDSSDKIAATACVKQFEVFGVKDTAFNFQDKVPIKFANSFAFGLNPVSIVEYEY
                     CVDKKVCEAIPQEGLNPDCNAY"
BASE COUNT         1031 a         1415 c         1654 g         1080 t
ORIGIN      
        1 gtccagatcg gcgaggacgg ctccggctgc cgcaagcacg gcgtatcgat ctggaccgag
       61 accgtcaagg cgcttgtgcc tgtcgaggac agcaagggca gcaccacttc cgcggtcgtc
      121 ggcaaaaggc ttcatgacta ctggcgtctg agcgttggcg gtggaagcga agggtcaatc
      181 attaattaca gtccggatca gattgcgtcg ctcgactccg accgttgctt tgccgtcagg
      241 aaccgtcttt acaaggcaag ccagcgcatc gagcagacgc ttcagatgca gaagaagaat
      301 cagtatatct ggttgaattt ccagaatatt ccgtgaccgg tttgacaact tccgcttgaa
      361 ggtgattgct ttgagtgaat caaacaggct gttcgcgcgc tggtcggctt tgaagaagga
      421 agtaaccgtc aaggacggtt gcggaatgcg tgttcttgat ctggggccgg gggcgattcc
      481 ccgtggtcgt cttgtgtgcg tcctgggacc aagcggttgc ggcaagacca gtcttctttc
      541 catccttggg cttgttgacc acgagtttga aggcgacctt ggcgtcgaac ttggcggcag
      601 gttccagccg atcggccagt ggcgcggcag aaagcgtcag cgcgagtccg cccgccttcg
      661 tcgccatatc gggttcgtat tccaggaaat gcgccttcgc gccggtgcgt cggccgtcga
      721 aaacgtcatg gatccgatgc tttacctggg attcggaacg ccccggacca ggtattcgca
      781 cgcgaagtcg atgctggggc tgtttggcct tcaggatcgc gatatcgaac gtccaatctc
      841 gtgtttttcc ggcggcatgc agcagcgcac cggcctggcc cgcgcgctgt cgatcggacc
      901 cgacctgatt atcgccgacg agccgaccgc gcacgtcgat gacaagatgg ccaatgccat
      961 ttacgaagat ttgaagcggc gttcggcgga aaggggaatc accgtcgttg tggcgaccca
     1021 caacgaggaa atggccgaca agcatgccga ccatatcatc cgcttcaagc cgaagttcca
     1081 ggacgaggaa gaagtccgtc gtgccaagga tctgggcgag tggccgtatg accttatcgt
     1141 cgagcgcgag gccgagggca tcgagcccgc cgatgcaaag ggcgagaccc cgaagcgcgc
     1201 ctcgactctt ggaagtttca aggatatggc cgtagtcgcg cttggcgagg gctatcccct
     1261 tgtaagggtt accggtcgcg ggattcgcgc ccttggctgg gggtgggcgg ccgccctggt
     1321 gaacaggctg cgcaagaagc cgtcggtcag acgcgaaaga atcgcgccgc accggtatct
     1381 tccgttactg gtgtcaatcg tgacctttgg tctgctggcc ggaatgggat tttcacttta
     1441 cggcatcaag tcggcgatcc ttgcgtacca gcagtccaag ttggagtcgc tcgaatccct
     1501 gcgtcgcgta agggtgttcg atccaggcga ccttgaaggc ggcgcgatac gccttgatgt
     1561 ggccgacgtt gaaaaattcg cggccgaaca cggcgttaag attaccgaaa gcgtgccggt
     1621 gcacgatctg ggcggcgagg ccctgatccc gtccgaaatg gcgcacctgg caagcgaggg
     1681 tcagtgggcg ccggttcagt cgtcggcgtt gctggaccca agctcgattg acgatcgcga
     1741 acggaatgcc aaccggatct tcttcacgat tcttggcact ttccgaacga gccacaggcc
     1801 agggatcagg gcttgccggc cgagccgagc gggttcgcgc caaaggacga tgctgcaccg
     1861 gccgtctgga ttccgcggaa cgagtgggaa tggctttacg gcaatttcga ggcgcttccc
     1921 gagccgggag attcggtcgg cgtcatgttc atgggcatcg cccgcatgga acgcaccccg
     1981 gaagcccagg gtgatgtcat cccgcccgcg gtgtgcgtgc gtttcaatat cggcggctac
     2041 ctgaatccgc cgcgcctgcc gtccggattc gtgtccgagg ttcaggccaa gtattacaag
     2101 gccgtcgttt ccaaggatgt cgtgaagcgc attcttgaat ggcagaacga tcccctgaac
     2161 cctgcgaagg ctctgcctga tgcctggcgg tgcgacggct cgccgtcgat ttatcccgac
     2221 tggcaataca ccgtcaagga cgaggtcagg gccccggcgg cgacccagtt cgatttcttc
     2281 gccccgacgc cgtcggacgc gctgaaactg gaaaggatcc tggaagacta ctcttccgat
     2341 cgcggcctga cgttcggcag ggtttcttcc gaacgcgggt tcatccagca ggtttcacgc
     2401 ctgatcaagc tggcagaata tgtcgggctt gtgcttcacg tcctgccgat cgccgtcggc
     2461 ggcgtcattc tgtggctggt cgttcacgag atcctgttcc gccggcgcga ggatcttctt
     2521 cttttccacg tgatgggcgc gccgaccatc aaccttcatg tccagtcgct ggttctttca
     2581 atgctggtcg tgctgccagg catccttatt ggttacatca tcggcagcgc ggggccgggt
     2641 cttatggtcg gcgtgatgtc caacgaaacc cttccacccg acctgcttga gcacctggcc
     2701 aaggtcggaa tcggctgggt cgggcttctg caaacgaccg tcgcggcggt catttgcgcg
     2761 gtttttgctt cgttcacggt cgtgaagtcg atcctttcgt cgaatccggc ttctgctttc
     2821 aggggtatgc aatgaagtca tcaaacaaca aggttgtaaa aacgatgaag tgtgcagtga
     2881 atgtggggac cctgacggtt ttcctgttac tgggcctgct gctggctccg gttgtggctt
     2941 ccgcggacgg cgcggccgag ttccgactgg tttcgggctt caagccgatg ctgcagaagt
     3001 gtccggccag cgatgcttgc gaagtcacgc tggtgaagga atacggcgca tggatgtggc
     3061 aggcaagttg caccgccaag ctgggcgagg acgttttccg caggctggac ctgttgaagc
     3121 gccgtgaagc cgacgtggtg gccattgtcg tttccaaggc aaagcgtgcc gtcgccgccg
     3181 acatccttga ctacatcgat gaaaggcgcc aggggcacct gctggattcg gggcggcttc
     3241 gcctggttcg tctggaggaa gtccagaggc acaaggttga tgaaaagctg cttgaactgt
     3301 gcgcccccgc ggtggctttg ccggatggga tgtccttccc gccgaacacc gcgatcgtct
     3361 tcgtcggtga caagggcgag gcaccgaaga gcaatccatg caaggacgag cgctggcgcg
     3421 atggcgtgac agttctttcg aatatggatg atatcgacca gatcgtcgga cgcaagctgg
     3481 acgacttgat cagtacgaaa agccagatcg aggacatcaa gcggcacgag cgcaaggcgt
     3541 gcgaggaaac cggtgacgcc agcgctttca tcgagcttgt tttcgacccg gtcgtgaagg
     3601 actccgccgg tgacagcccg aacctgtcgg cgtccgacct gggcctggac aacgccgaga
     3661 tcgttacgca gttgcgcaag atgggcgtcg agatttcgtc acagctttca agccttttcc
     3721 cggccgatgt tgaaagatcc gcgatgaagc gtgaagacgg caagtgggcc ttgaacgcac
     3781 agaagctgcg atcgttgatg gcactggccg acagcagggc cggcgaaaag atgctgaagc
     3841 aggccagcgc caagttgatt gcccagattc aatcggccat gacacgcgtc gaacgtttct
     3901 cttcgactta cagcggcgga aaggggccgg atttgtcgat cgatgcgatg aaggccagga
     3961 acgaactggt tacgcgcaag tacggccagg tttgcggggg ttcgggcatc accgccgagt
     4021 gctcgtcggc gcgtgccagg attgatgaac tggaggccag gctggaatcc tttgaaaagg
     4081 agccggcacc catcgcggca tccccggttg cccaggccgc cccggcaaag ccaatcgacg
     4141 agtggagtcg tgaagccacg gcgtccctgc agacgctgca gggcaacgtg gttttgtgca
     4201 agaccaggga atgcctggat ggccaggcga ttgaatacgc ccgtcttaac ggctcgatcg
     4261 aaaggtacca aagggaatac ggcgccagcg tcgccgcggg gaagggggtt gaccccagga
     4321 ttgtggagct tgagaacctg atggcttcgg ccgaggccga ccttggcgca gcccgcggca
     4381 cgccggccag ttggtcgaag atgaagattg gtggcgttgc cgcgctttcc gccggcggcc
     4441 tgataatgat cgccgccatc gttccgctgc tgatggcgaa ttccgaaatg aagaagattg
     4501 aagacgcgac caacggcggg attgtgccaa gagcatggtt aagtattgat ggttttgggg
     4561 atcagtggtg gtacaggatc aacaaatttg atgtcgacga cattggagca atggtgaccc
     4621 gccagcgcat gaaggccatt atcggatggt ccgttcttgg cgccggcgca ctggccgcga
     4681 ttgccggcgg cgtgctgctt ggcgtcgatg cgatgaagga aggcaggaac aagcccaccg
     4741 taacggtcgc cccggttgaa tccggcgccg tcgtcggtgt caacatgtca ttctaggagg
     4801 ctgtcagatg aaacatacag attcaatgtt tgacaaggtt gccgccgccg catgtatcgt
     4861 cgttcttggc gccgtcggtt gcgtggccga gcagttcgaa tgtgatgaca ataaaccgtg
     4921 cagcgaatcc ggtgaggtgt gcgtttatta tcttgactcg tcagataaga ttgcggcaac
     4981 agcctgtgtc aagcagttcg aggtgttcgg ggttaaggac acggcattca acttccagga
     5041 caaggttcct atcaagtttg ccaatagctt cgcatttggt ttgaaccccg tttcgattgt
     5101 ggaatacgag tactgcgtcg ataagaaagt atgtgaagcc atcccgcagg aagggcttaa
     5161 cccagattgc aacgcttaca
//
LOCUS       DAOPPA010000211         5135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17679_length_5135_cov_5.618701, whole genome shotgun sequence.
ACCESSION   DAOPPA010000211 DAOPPA010000000
VERSION     DAOPPA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17679_length_5135_cov_5.618701"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKH54_07435"
     CDS             complement(<1..685)
                     /locus_tag="PKH54_07435"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOC99761.1"
                     /translation="MIDFKPRLAAALIIAVCGMSVVPQVAPGADGDFFRDSIVADLHC
                     DALFQLSRKDRKVKPGDLMCGPDRLAAGNVDAQVFVIWVPPDSADPKAVAADQVRAFR
                     NVLLSRKGRLEQARTSEDVRRISSRGKIAAMLGLEGCTPLGGEPDALAEFASQGIVYV
                     SLTWNETNDFGQGNSGRKPAGITARGRRLVELANSLRVIPDVSHASPSTFRGVMTTST
                     RPVIASHSNS"
     gene            complement(682..>1623)
                     /locus_tag="PKH54_07440"
     CDS             complement(682..>1623)
                     /locus_tag="PKH54_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOC99762.1"
                     /translation="DRKLKPASRLILEDYLRSQISSFGKFTVIATAEVERALSQIRVE
                     SHRDCVDDRCQIELGEILSASQALSITVMALDKNCGIIAHMNDVKRSAMSGSAEAMDV
                     SCDRVGLMKGLRMVAAKLSGQPVPPDVLPPPPPPVEPIQRIQSVQPVQQAPVVARPPS
                     RLHISSVRVSSELPSPKNYNAARNAIDGRPDTWWGEGAGGDGIGEWIEASLGRQAEIR
                     EVRLIPGYQKSLPDKFGDRWPLNNRLRKVRVELDDGSSFVAELSDRKGFHSIAIPSGN
                     FSRSVRIVILEVFPGYNQKGERIQDSGIAEIQILGVE"
     assembly_gap    1624..1723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1724..2266)
                     /locus_tag="PKH54_07445"
     CDS             complement(<1724..2266)
                     /locus_tag="PKH54_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99763.1"
                     /translation="MFCATSPRHSYMAISAFLFIGAFAAIVGVPSAAEAQDPTHQNEQ
                     LQEFDLNYPEDGTAPERRYQMKPYEEYHEPTQNPAADANRARMLDRLTKIGSELHQAK
                     RLEKIGWPLFAILTPIAGACLGAGVAYDFIAADGVAEGIGSLLGLGIGGLIIGSIGSA
                     RANRLKREQKKILSEINPDGG"
     gene            complement(2356..3642)
                     /locus_tag="PKH54_07450"
     CDS             complement(2356..3642)
                     /locus_tag="PKH54_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOC99764.1"
                     /translation="MRRFILLAGAVLALSGCDNTVDPAGMQVVSSDMSRDMAPSGQGI
                     ETLTAGNSAFGFELYLDLAARNEGKNVFISPHSVSSALAMAYAGARGETASQMRQTLG
                     FGEQPESTFEAFNALDLELDRRGSEQLQADEKGDPFELSVVNQAWGRIGFEFLQPYLD
                     VLAVNFGAGLRLMDFTNDPDGSRVVINDWVAEQTRDRIPDLLPEGSISPATALVLTNA
                     IYFKASWRNKFDKEDTVDDEFTRLDGATVGVKMMKAYDELRHFVGDGYEYLEIPYVGD
                     KVVMALILPDAGRFDEVEAGLSGDAFASMVQGSVDAAGYLRLPRFTFEFESSLNDSLK
                     AMGMTAAFSAGGADFSGIDGIPGNLFISLVQHKSFVAVDEEGTEAAAATAVVMDETSA
                     PMDTFDMTFDRPFMFAIVDQLTGAVLFVGRVVDPSA"
     gene            3836..4723
                     /locus_tag="PKH54_07455"
     CDS             3836..4723
                     /locus_tag="PKH54_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99765.1"
                     /translation="MNKSFKTVSFKAFTAAFLTAALMAAGCGSNDQNNDVGEQQDVVS
                     DQGGNTDTNQPLDVQNPDEGQPDTNQPLDVQNPDQGQPDTTTAANPHDYWPGVDWDTF
                     FPGPEKSAVYQVTTFDQVQKDLTARVVYDVEWKGGTWTQIVVGDLEPGKDGMAIYFDK
                     SEPWVIKAKGVVVYGENVTDGPLMTEFFTDPIVIPLNKDTGDSQEIDTQINGEYDGFV
                     DDMGVTYAIEFTSFDSEVTVPAGTYSGCADLKATLGGELMGGTQTIDVEIKIHPKQTI
                     VHWVDSPGFGLAELKTEWK"
     gene            complement(4742..>5135)
                     /locus_tag="PKH54_07460"
     CDS             complement(4742..>5135)
                     /locus_tag="PKH54_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC99766.1"
                     /translation="AAVQGALGGLIFLILGAPHPLFWGVVMAVLSLIPVVGSGVVWAP
                     YAVFLIATGHPVRGVVLIVFGVVVIGLADNILRPILVGRETRMPDFLVLLSTIGGLAV
                     FGVNGFMFGPLVAALFLAVWDIFEKENA"
BASE COUNT         1131 a         1549 c         1435 g          920 t
ORIGIN      
        1 ccgagttgga atgactggca atgacgggac gcgtgcttgt ggtcatgacg ccccggaaag
       61 tcgatggcga tgcgtgagaa acatccggaa tcacgcgaag gctgttcgca agttcaacaa
      121 gcctgcggcc cctggcggta attccggccg gtttcctgcc agaattgccc tggccgaaat
      181 cgttcgtttc gttccaggtc agggatacat acacgatgcc ctggctcgca aattcggcca
      241 gagcgtccgg ctccccgcca agtggcgtgc aaccttcaag accgagcatg gcggcgattt
      301 ttccccggct cgagatcctc ctgacatcct cggacgtcct tgcctgctcg agccgcccct
      361 tccgggaaag caggacattt cgaaacgccc tgacctgatc ggcggcgacg gccttcggat
      421 cggccgaatc cgggggaacc cagatcacga agacctgggc atcgacattt ccggcggcaa
      481 gacgatcggg gccacacatc aggtcgcccg gcttgacctt ccgatccttt cgcgaaagct
      541 gaaaaagcgc gtcacaatga agatcggcaa caatcgaatc ccggaagaag tcgccgtcgg
      601 cgcccggggc aacctgcggc accacggaca tgccgcagac ggcaattatc aacgcggcgg
      661 caagcctggg cttgaaatca atcactcgac acccagtatc tggatctcgg cgattcccga
      721 gtcctggatc ctttcgccct tctggttgta tccgggaaag acctcaagaa tgacaatcct
      781 taccgatcgg ctgaagtttc cggacggaat tgctatcgaa tgaaagccct ttcgatctga
      841 aagctcggcg acgaaggacg acccgtcgtc cagttcaact cgaaccttgc gcaaccggtt
      901 gttaagcggc caccgatcgc cgaacttgtc gggcagactt ttctgatatc cgggaatcag
      961 cctgacctcg cgaatctcgg cctggcgccc cagggacgcc tcaatccact cgccgatgcc
     1021 gtcgccgccg gcgccttccc cccaccacgt gtcggggcgt ccatcaatgg cattgcgcgc
     1081 ggcgttgtaa ttcttcgggg acggcagttc ggacgatacc cgcaccgaac tgatatgaag
     1141 acgggatggc ggccgcgcga ccaccggcgc ctgctgaacc ggttgaacag actggatccg
     1201 ttgaatcggc tcgacgggcg gcgggggcgg tggcagcaca tcgggcggaa ccggctggcc
     1261 gctgagcttc gcggcgacca ttcgaagccc tttcatcaat ccaacccggt cacaggaaac
     1321 atccatggct tcggccgaac cgctcatggc ggaacgcttg acgtcgttca tatgggcaat
     1381 gatgccgcag ttcttatcaa gcgccatcac agtaattgaa agagcctggc ttgccgaaag
     1441 aatctcgcca agctcaatct ggcaacgatc atcgacacag tcccggtgcg attcgacgcg
     1501 gatttgggac agggctcttt caacctccgc ggtcgcgata accgtgaatt tcccaaagct
     1561 tgaaatctgg gaacgcaggt agtcttccag tatcagcctg ctggccggct tcaacttgcg
     1621 gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcccccg tccggattga
     1741 tttcggaaag aatcttcttc tgctcacgct tcagccgatt ggcgcgggcc gaaccaatcg
     1801 acccgattat aagcccgccg ataccaagac ccaagaggga tccaatgccc tccgcaacgc
     1861 catcggcggc gatgaaatcg tatgccacgc cagcaccaag gcaggcgccc gcaatcgggg
     1921 tcaaaatggc gaacaatggc cagccaatct tttcaaggcg cttggcctga tgcagttcgg
     1981 agccaatctt cgtcagccga tcaagcatcc tcgcccggtt ggcatcggcg gccggattct
     2041 gtgtcggttc gtggtactcc tcgtaaggct tcatctgata gcgacgttca ggggccgttc
     2101 cgtcttcggg ataattgaga tcgaattcct gaagttgctc gttctgatgg gtgggatcct
     2161 gggcttctgc cgcggatggc acgccgacaa tggccgcaaa ggccccaatg aaaaggaatg
     2221 cggaaatagc catgtacgaa tggcgtggac ttgttgcaca aaacatcgca caacccggca
     2281 atcaagagtt gggcggattc tgtgactgcc cgaaaactat gtcaactgcc gaatgctgga
     2341 aatcagcgag atttgtcatg ccgacggatc gaccacacgc cccacgaaga ggacggcgcc
     2401 agtcaactga tcgacaatcg cgaacatgaa tggacgatca aacgtcatgt cgaacgtatc
     2461 catgggcgcg gacgtttcat ccatcaccac ggcggtggcc gccgcggcct cggttccttc
     2521 ctcatcgacg gcgacgaagc tcttgtgctg caccaggctg atgaaaaggt ttccgggaat
     2581 gccgtcgatt cccgaaaaat cggcgccccc ggcagaaaag gcggcagtca ttcccatcgc
     2641 cttcagcgaa tcgttcaagg acgattcaaa ctcaaacgtg aagcgcggca gtctcaaata
     2701 gccggcggca tccaccgaac cctgaaccat cgatgcgaag gcgtccccgg agaggccggc
     2761 ctcgacctcg tcgaaccttc cggcgtcggg aagaatcagt gccatgacaa ccttgtcgcc
     2821 aacgtacggg atttccaggt attcgtaccc gtcgcccacg aaatgccgaa gctcgtcgta
     2881 agccttcatc atcttcacgc cgacggtggc tccgtccagg cgcgtgaact cgtcgtcaac
     2941 ggtgtcttcc ttgtcgaact tgtttcgcca gctcgccttg aagtaaatcg cattcgtcag
     3001 gaccagcgcg gtcgccggcg agatgctgcc ttccggaagc aggtcaggaa tacgatccct
     3061 tgtctgctct gcgacccagt cgttgatgac gacacggctg ccgtccgggt cgttcgtaaa
     3121 gtccatcagc cgcaggccgg cgccgaagtt tacggccagc acgtcaagat acggctgaag
     3181 gaactcgaac ccgatgcgcc cccaggcctg gttgacgacg ctcagttcga acgggtcgcc
     3241 cttctcgtcg gcctggagtt gttccgagcc tcgcctgtca agttccagat ccagcgcgtt
     3301 gaacgcctcg aacgtggact cgggctgttc gccgaacccg agcgtctgcc gcatctggct
     3361 ggcggtttca cccctggccc ccgcataggc catggcaagc gccgatgaaa cgctgtgcgg
     3421 cgaaatgaat acgtttttcc cttcattacg cgccgccaga tcaagataca gctcaaaccc
     3481 gaaggccgag ttgccggcgg taagcgtctc gatgccctga ccggatgggg ccatgtcccg
     3541 ggacatgtcg gacgacacaa cctgcattcc cgccggatcc acggtgttat cgcacccgga
     3601 aagtgccagc acggcgccag ccagcagaat gaacctccgc atgatcgacc tccatccgcg
     3661 ggccatcgtt aatcggcccg gaaacattga aaaccgcttt cgggaagacg gtgatgattg
     3721 tattacttca agcgccggca aacaaacgtc cgggcctgga catccagact tgaaaagccc
     3781 cgccgcgccg ttaaaatacc agtgtcacaa ataacacctt ccgggggata aaacgatgaa
     3841 caaaagcttc aagactgttt ccttcaaggc tttcaccgcc gcattcctga ctgccgcgct
     3901 gatggcggcc gggtgcggat cgaacgacca gaacaatgac gtcggcgaac agcaggacgt
     3961 cgtgtccgat cagggcggca atacggacac taatcagcct ttggacgtcc agaatcccga
     4021 cgaaggccag ccggacacga atcagccttt ggacgtccag aatcccgacc aaggccagcc
     4081 ggacacgacc accgcggcaa atccgcatga ctactggccg ggggttgact gggacacgtt
     4141 cttccctggc ccggaaaagt cggccgtcta tcaggtcacg acgttcgacc aggtgcagaa
     4201 ggatctgacg gcccgcgtcg tctatgacgt cgaatggaag ggtggaacct ggacccagat
     4261 cgtcgtcggc gacctggaac cgggcaagga cggcatggcg atctattttg acaagtcgga
     4321 accgtgggtc ataaaggcaa agggtgtggt cgtctatggc gaaaacgtca ccgacggccc
     4381 tctgatgacc gagttcttca ccgacccgat cgtcatcccg ttgaacaagg acactggcga
     4441 ctcgcaggaa atcgatacgc agattaacgg cgaatacgac ggtttcgtcg atgacatggg
     4501 cgttacctat gccatcgagt ttacgtcctt cgattccgag gtcacggttc cggccgggac
     4561 atactccgga tgcgctgacc tgaaggccac gctcggcggc gaactgatgg gcgggacgca
     4621 gacaattgac gtggaaatca agatccaccc caagcagacc atcgttcact gggtggacag
     4681 ccccggcttc ggactggccg aactgaagac cgagtggaag tgaacgtcgc tcgattgcgc
     4741 cctaggcatt ctccttttca aaaatgtccc agacggccag gaagagtgcc gccacaagcg
     4801 ggccgaacat aaatccgttg acaccgaaga cggccaggcc gccgatggtc gaaagcagga
     4861 ccaggaaatc gggcatccgc gtttcgcggc cgacaaggat cggacgcagg atgttgtcgg
     4921 ccagcccgat cacgaccacg ccgaacacaa tcagcacgac gcccctgaca ggatgaccgg
     4981 tggcgataag gaaaacggca taaggcgccc acacgacacc ggaaccgacc acgggaatca
     5041 gggacaggac ggccatcaca acgccccaga aaagcgggtg cggggcaccc agaatcagga
     5101 atatcagtcc cccgagcgct ccctggacgg ccgcg
//
LOCUS       DAOPPA010000212         5131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17695_length_5131_cov_3.887707, whole genome shotgun sequence.
ACCESSION   DAOPPA010000212 DAOPPA010000000
VERSION     DAOPPA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17695_length_5131_cov_3.887707"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..2868)
                     /locus_tag="PKH54_07465"
     CDS             complement(115..2868)
                     /locus_tag="PKH54_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOC99767.1"
                     /translation="MSRFFNPAFVKALIARRWFRVSCAVVSVAGALWVAFSLVIFPVL
                     MDSVLQTLNRRLNSVSGDSSLSVGGIEPFRGQEIILNDLNFVVSGRSVLQIPVVRLGF
                     SAIDVIGGQRVPEYVIVERMAVILDTDFDEWGGLKPLKARLDDIRSRPSDVTAADGGP
                     SRLKLPSLTIHDSSVGLIHRDTGETIVSLSGIGLKVAPHDYGAGAGGLVGFSLDGRMT
                     LTGLETVQGGNEADLEVSGLVQGDGGFSLKMSSGIPFYAPRNRRLPAVMSLGSLEVRM
                     DVAAGRLSASLGDLSVGGLGQFVDKASHGRLQLEGELGMDSAGIELKLADIRHLSTVS
                     VSDLVNLSLSGARFSARFQDGKTINARDIGLTMTPVSTEAASEPAGEAGAPFWVVDFS
                     GLAGTREDDFSAVSLTGRFSRELAFIDGHLKVDGSLPIVVLSIFHNRLLPWGAPSLGL
                     DVDVINDGQFWKMKGHARASNLSYFVPRLCLVPITGVSFETDFDLLASVSEKVINVSV
                     DAFKIGSMELSASLEYEFMHAQPSVKTTLTIPRQDCQAVFEAIPPAMVPRLIGAKASG
                     QMSFGIKFEAMPGRQSVFKVDADLDDCRVQTLGKAVNLRRLMSDSYVHKVVEQDRDGR
                     TVVIYTGPGSSSFVPLDKIPVHVQQAALATEDMRFFKHRGFAPGLIKRAILLNMDKGW
                     YVYGGSTITQQLVKNLFLSREKTMARKLEEAIIVWALEREIPKERTLELYLNCIEFGL
                     NIYGIRRAAEVYFNKTPMELTPLEAAFIMATKPNPKSAFNVYEKRQMGKWWVERLEGI
                     LKRLWGEMSQISEREYVDAYPWIPVFWYESDGIYAQPAIVGVPDVPSMDALGLAPIVN
                     PPIPQPVPVPAAGDLDGADQDDGGDDWRDDGLEGGENDWERRQREDREWRMRERNDRT
                     EDE"
     gene            complement(2918..3838)
                     /locus_tag="PKH54_07470"
     CDS             complement(2918..3838)
                     /locus_tag="PKH54_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOC99768.1"
                     /translation="MDSHEEGRTCTSLRNIVDALCEVEGNRWARADWSDPRRLPSRDD
                     LIRFVDMIRAVLFPGYFGSADMSRDSMRFHVGAALDEAMQILHEQLTRGQCFLMCRAA
                     DACGSTGTGDCSECREKSVAIARDFMERLPEIRRRLNTDVQAAFEGDPALHSPDEAIF
                     CYPGLQAVISQRIAHELFILGAPLIPRIITEHAHSMTGIDIHPGASIGDGFFIDHGTG
                     VVIGETCVIGNNVRIYQGVTLGAKSFPLDENGNPIKGIPRHPIVEDDVVIYSGATILG
                     RITIGKGSVIGGNVWVTSDVSPGSVISQKR"
     gene            3939..>5131
                     /locus_tag="PKH54_07475"
     CDS             3939..>5131
                     /locus_tag="PKH54_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOC99769.1"
                     /translation="MKKINLAILWHMHQPPYLEPFSRQNLLPWAFLHGMKDYHDMGAT
                     VLRHPAMRINVNFTASLLDQLEGYADGTTVERTLQVMSRNPSELAEAELEYLYRTCFG
                     GTDLMAAGLPAFVRLRNLYRAAVASENRVPRLKPNEITDLTVLYLLSWCGPTLSATST
                     VRALVEKGQGFSPADRDLLIDEARKLIKATKGLYGDLSKSGIVELSTTPGTHPILPLL
                     CSTAAAAEARADVVLPPSQFDSPEEARRQVETGLARFESYFGFRPAGMWPAEGSVSPQ
                     AVSIIGSAGIRWIATDEAILARSSADVPVSPRRPWTYNGVSIFFRDHFLSDQIGFVYS
                     RRPAEKAVQDFLDEMKARAATARSEDELVVIALDGENAWEYYQDGGYPFLDRLYGAIV
                     DSDFI"
BASE COUNT         1127 a         1619 c         1402 g          983 t
ORIGIN      
        1 gccccatggt tctgacgtac cgacgcacca gcacctggcg ggactcgtcc ataaccacga
       61 cgttgagact aacggaaccg acgtcgattc caagatacct tgccatgtca gcctctactc
      121 gtcttcggtg cggtcattgc gttcccgcat gcgccattcg cgatcttccc gctggcggcg
      181 ctcccagtcg ttttcgccgc cttcaagtcc atcgtcacgc cagtcatcgc cgccatcatc
      241 ctgatcggct ccgtccagat cgcctgccgc cggcaccgga accggctgcg gaatcggtgg
      301 attgacaatc ggcgcaaggc cgagcgcatc catggacggc acgtccggaa ccccgacgat
      361 cgccggctgc gcgtaaatcc catcggactc gtaccagaac accggaatcc atggataggc
      421 atcgacgtat tcacgctcgg aaatctggct catctcgccc cacagcctct tcaggatgcc
      481 ttcaagccgc tcgacccacc acttgcccat ctgtctcttt tcataaacat tgaacgcgga
      541 ctttggattc ggcttcgtcg ccataatgaa agccgcttcc agcggcgtca gttccatcgg
      601 cgtcttgttg aaatagacct cggctgcccg acggatcccg tatatgttca ggccgaattc
      661 gatgcagttc aggtacagtt caagggtgcg ttccttcggg atttcacgct caagcgccca
      721 gacgatgatc gcttcctcca gcttgcgggc catggtcttt tcccttgaaa ggaaaagatt
      781 cttcaccagt tgctgcgtga tggtgcttcc gccatagacg taccagccct tgtccatgtt
      841 cagcagtatc gcccttttga tcagtccggg ggcgaacccg cggtgcttga agaacctcat
      901 gtcctcggtg gcaagcgcgg cctgctggac atgaaccgga atcttgtcca gcgggacgaa
      961 gctgctgctc cccgggccgg tgtaaatcac caccgttcgg ccgtcacgat cctgctcgac
     1021 aaccttgtga acgtacgaat cggacatcag ccttcgcagg ttgacggcct tgcccagggt
     1081 ctgaaccctg caatcgtcaa gatcggcatc gaccttgaag accgactgtc ttcccggcat
     1141 cgcttcgaac ttgataccga aggacatctg tcccgacgcc ttcgcgccga tcagacgtgg
     1201 aaccatcgcg ggcgggatgg cttcgaacac ggcctggcag tcctgccgcg gaatggtcag
     1261 cgtcgtcttg acgcttggct gcgcgtgcat gaactcgtac tcaagcgatg cggaaagttc
     1321 catcgatccg atcttgaacg catcaaccga aacgttgatc accttctcgg aaaccgacgc
     1381 cagcaaatcg aaatcggttt cgaacgaaac cccggtaatc ggcacaaggc acaaccgggg
     1441 aacaaaatac gacaggttcg atgccctggc atgacccttc atcttccaga actggccgtc
     1501 gtttatcaca tcaacgtcca gtcccagcga aggcgcgccc catggcagca gccggttgtg
     1561 gaatatggac aggacgacga tcggaaggct tccatcaacc ttcaggtgac catcgatgaa
     1621 cgcaagctcg cgtgaaaaac ggccggtcag cgaaacggct gagaagtcat cttcccgagt
     1681 tccggcaagc ccggaaaaat caacgaccca gaacggggcg ccggcttcgc ccgcaggctc
     1741 actggcggcc tccgtcgaga cgggggtcat cgtaagtccg atgtcgcgcg cattgatggt
     1801 cttgccatcc tggaagcggg cggaaaacct ggcccccgaa agggaaaggt tcacaagatc
     1861 cgaaaccgaa acggtcgaca aatgtcggat gtcggccaat ttcaactcga ttccggccga
     1921 gtccatcccg agttcgccct caagctgaag cctgccatga ctggccttat cgacgaactg
     1981 ccccaggcca cccacggaaa gatccccaag cgaagccgac agcctgccgg ctgcaacgtc
     2041 catcctgacc tcgagcgaac cgagcgacat cacggcaggc aggcggcgat tcctgggcgc
     2101 gtaaaacggg attcccgagg acatcttcag ggaaaaacca ccatccccct gcaccaggcc
     2161 ggacacttca aggtctgcct cgtttccgcc ctgaacggtt tcaaggccgg tcaacgtcat
     2221 ccggccatcc aggctgaacc cgacgagtcc gccagcgcca gcaccgtaat catgcggggc
     2281 aaccttcagg ccgatcccgg aaaggctgac aatcgtctca ccggtatcgc gatgaatcag
     2341 cccgacggac gaatcgtgaa tggtcagcga aggaagcttc aaacgcgacg ggccgccatc
     2401 tgcagccgtg acatccgaag gccgggatcg aatgtcatca agcctggcct tcaacggttt
     2461 caatccaccc cattcgtcga agtccgtatc caggatcacc gccatccgtt cgacgatcac
     2521 gtattcgggg acacgctgcc cgccaatgac atcgatagct gaaaacccca gccggactac
     2581 cggaatctgc aggacactcc ttccagatac gacgaagttc aggtcgttca ggatgatttc
     2641 ctgaccgcgg aaaggttcga tcccgccaac tgacagcgaa ctgtcgcccg aaaccgaatt
     2701 caggcgccgg ttcagggtct ggagcacgga atccatcagc accggaaaaa tgaccaggga
     2761 aaaggccacc cacagggcgc ctgcaaccga aacgacggcg caggataccc gaaaccacct
     2821 tctggcgatt aatgccttca caaaggcagg attgaagaac cgggacatgg ccgagccctt
     2881 tcagaccggc gcattttatc accaaacagg cctgacctta acgtttctgt gaaatgacgc
     2941 ttcccgggga cacgtccgag gtcacccata cgttgccgcc gatgaccgag cccttgccaa
     3001 tcgtgatgcg cccgaggatg gtggccccgg aataaatcac cacgtcgtcc tcgacaatcg
     3061 ggtgacgcgg aattcccttg attggattgc cgttttcatc aagcgggaag ctcttggcgc
     3121 caagggtgac gccctggtaa atccggacat tgttgccgat tacgcatgtt tcgccgatga
     3181 cgaccccggt tccatggtcg atgaagaacc cgtcgccgat cgacgcgccg gggtggatat
     3241 cgattccggt catggaatgg gcgtgctccg tgatgattct gggaatcagc ggcgcgccca
     3301 gaataaaaag ctcatgggca atacgctggg aaatcactgc ctgcagtccg ggatagcaga
     3361 aaatcgcctc gtccggggag tgaagcgccg ggtcgccttc aaaggcagcc tggacgtcgg
     3421 tgttcagacg acgccggatc tcgggcagcc tttccatgaa atcgcgggca atcgccaccg
     3481 acttctcgcg acattccgag caatccccgg ttccggtgct gccgcaggcg tccgccgccc
     3541 tgcacatcag gaagcactgt cccctggtaa gctgctcgtg cagaatctgc atcgcctcgt
     3601 ccagagccgc cccgacatgg aacctcatgg aatcccggga catgtcggca cttccgaaat
     3661 acccggggaa aagaaccgcc ctgatcatat cgacaaaacg gatcaggtcg tcgcgcgagg
     3721 ggaggcggcg cggatccgac cagtccgccc tggcccagcg gttgccctcg acctcgcaaa
     3781 gggcatcaac gatatttctt aacgacgtgc acgtcctgcc ttcttcgtgg ctgtccattg
     3841 ccgacacctt gattcaaagt acgttacaaa cttattattc gtgaccccgg cgttgtcaac
     3901 atcacgccgg caggaaacac ggagagcgga cggcaacgat gaaaaaaatc aacctggcca
     3961 tactctggca catgcatcag ccgccgtacc tcgagccgtt ttcgcgccag aaccttctgc
     4021 cgtgggcatt cctgcacggc atgaaggatt accatgacat gggggccacc gtcctgcggc
     4081 acccggccat gcgcatcaac gtcaacttca ccgccagcct gctggatcaa cttgagggat
     4141 acgcagatgg aacaaccgtc gagcgaaccc tgcaggtgat gtccagaaac ccgtccgaac
     4201 tggccgaggc cgaactggaa tacctttaca ggacatgttt cggcggtacc gatctgatgg
     4261 ctgccgggct gccggccttc gtgagacttc gcaaccttta ccgggccgcc gtcgcttcgg
     4321 aaaaccgcgt tccgcgactc aaacccaacg aaatcactga cctgacggtt ctttatcttc
     4381 tttcatggtg cggaccgacg ctgtcggcaa catcgaccgt acgtgcgctg gttgaaaagg
     4441 gccagggctt cagtccggcc gatcgggatc tgctgattga cgaagccagg aaacttatca
     4501 aggcaacaaa gggtctttac ggggatctgt caaaatccgg tatcgttgaa ctttcaacca
     4561 cgcctggaac acatcccatc ctgcctctgc tgtgttcaac ggcagcggcc gccgaagcca
     4621 gggcggatgt cgtcctgccg ccgtcgcaat tcgactcgcc ggaagaagcc cgtcgccagg
     4681 tggaaaccgg acttgcgcgt tttgagtcgt acttcgggtt cagaccggcc ggcatgtggc
     4741 ctgccgaagg ctcggtatcg ccccaggcgg tctcgatcat cggatctgcc ggaatcaggt
     4801 ggatcgcgac cgacgaagca atactggcgc gctcatccgc cgacgttccg gtcagcccgc
     4861 gccggccatg gacatacaac ggggtctcca tcttcttccg cgaccatttt ctttccgacc
     4921 agattggctt cgtttacagc cggcggcctg ccgaaaaggc tgtccaggac tttctcgacg
     4981 aaatgaaagc cagggcggcg acggcccggt cggaagacga actcgttgta attgcactgg
     5041 atggcgaaaa cgcctgggag tactaccagg acggagggta cccgttcctg gacaggcttt
     5101 acggcgcaat cgtcgattcg gacttcatca a
//
LOCUS       DAOPPA010000213         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17849_length_5097_cov_13.933756, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000213 DAOPPA010000000
VERSION     DAOPPA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17849_length_5097_cov_13.933756"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKH54_07480"
     CDS             complement(<1..398)
                     /locus_tag="PKH54_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99770.1"
                     /translation="MIGIAARFKCLSSVVAVLLTAASVCLGCSNATRYLKGPMPVSAL
                     PAPTWSISAPEDLAEAFANSPFLTFSAIATGIEPGVPPGLGFRIHLRNIEPSSEWLAP
                     NVWITVRTWVSVRPRSIPGKRVTVVVANGPT"
     gene            473..1660
                     /locus_tag="PKH54_07485"
     CDS             473..1660
                     /locus_tag="PKH54_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC99771.1"
                     /translation="MNRKMLLPPLGLLLVAGMILAAAAPARAAVLPLKVSADNLDEAT
                     TGELTKAIADKFARYPSLTLLPMPEADAIDVMIDAGCEDLNTECLVLIGKQYGATAVV
                     YSTAVGMDEGIGLSIRYVEVESGKQRVQDRAAASGVGPSDLMKLALGEILGPEPSTEP
                     AGVLVNFVSFPPGGEVYVGPEFVGIAPVSTRLSPGSHDIRMVKEGYRTRDGRIEVATD
                     KEAEVKWSLSAIPAQPVVAAPVAAPTPAPIPASTPALTPASTPAEPTPVASAPATAAT
                     STSAPASAPTTATTPATVATTPATVASKPATVASIPSDQPAVIDDVPTIPPEEQSSNE
                     KSSVSKQPFYKTWWFWTIIGVAVVGAGTAAGVLATQGEPAGGSIGFSPDPGFAPLDVT
                     IFR"
     gene            1686..3620
                     /locus_tag="PKH54_07490"
     CDS             1686..3620
                     /locus_tag="PKH54_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99772.1"
                     /translation="MMVRFFAVSLIMPALLLAGCAGSVDCAGGTCFYGPSDQQGAVSF
                     ALSGSDTNVRSFIIKLYKGATERARDNAYFQSSCSPLSKGFTISHVDVGDDYVVEYLG
                     YSDGACTPTTLVKQGVRGGVRITTLGTGFSYYFIQVNTVGDFTAMPTPDQTLNPSTGG
                     VSCQSDSDCRAVIPCPEPSKCRFKVNVDCTSAEIEAGECPDGVKVMQYNVHPRAVCDV
                     DTCRLKTLFPLNTRGDRAFASTVRTGSGDVVSFGGFTGKRDGFYVVEGASDPASAPET
                     QMFSGDSGIFGVLEHDKPLDSGVGMSSSVLLDDGRFVVVGGTSKALAATGSDFVGAPE
                     LESIFCTSDCPVQMSSYLYVIDSQTGEVDRTQLAEAVMPAAVLQVARPTPSVYVRAAA
                     SMKDFAGDRASGASTISWLCQVSDSNAVSCSDVGPSNPVSRFMPASVCIKRSAGLCSE
                     LLTLGGTDNISKFAEIYYADTNQVKVFPGTGDVPAYLTGAVAFMAGDRIWLVGGTDDP
                     DSTRTARPYEVSFERDLGVVVLSEVELPLSQADALGRIHHQVTVLGDDDHVLVTGGMN
                     QDGVVSSGAVLLKFDAGRMDVVDDSLSMTTARMGHSATLIGGGLYDGSVLITGGISTA
                     SGDFADGAEVYVPAFQGTGH"
     gene            3621..>5097
                     /locus_tag="PKH54_07495"
     CDS             3621..>5097
                     /locus_tag="PKH54_07495"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOC99773.1"
                     /translation="MRLRLPLKVLLPLAAMIALAVCLLVFNTVFALLLIVLVTVFTLI
                     GGATVRHAALADSGVNGAPRLLVKGTDRFVLTWVLVTALLWTFCAPDVYFRDAGELTG
                     AAVNLGVPHPTGFPLLCMPGKLFSLFPAGNVYFRFNLLSGFAAGGAAAFLPLFFERVF
                     ASDRSDQTGVWWVGPLIYLGSAAAWLHGTTPEIYSISLFGMLVTIFLALEAARANDAR
                     LLVASAVLSGLGLGGHVTWPLYSLPVVVAALAVFSFRAASGQRARTFFRVGFLSLFAF
                     VIGAMVVFYLVSAASRDPLMNWGDPSTFKGLLAHLSGQRIRDSFAGRMTSLNPAVLLV
                     NLSLAIRSLWDSLGPVLPFALVGALHAILRRSWWTTSVLVLVLADLVFAVFINPMGIR
                     DLQVLLTATWGLALLAASGASLAVAFLGRRGGAPGAVAMIGLVCVSLVFQWVTSPADR
                     DMRNLYGGRIISDILLDKAGPGAVAMTASDDMSALFMARTAV"
BASE COUNT          841 a         1511 c         1593 g         1152 t
ORIGIN      
        1 gtcggaccgt tggcaactac gacagtcact cgttttccag gtattgaacg cggccggacc
       61 gagacccagg ttcggactgt aatccagacg ttcggggcca gccattccga ggagggctcg
      121 atgtttctaa ggtgaatcct gaaccccagc cccgggggca caccgggttc gataccggtc
      181 gcgatggcgg agaacgtcag gaaagggctg tttgcaaagg cttcggccag atcctctggg
      241 gcgctgatcg accatgttgg cgcaggcaag gcagagactg gcattggacc cttcaggtag
      301 cgggtcgcgt tggagcagcc gaggcagaca gaagctgctg ttaaaagcac cgcgacaacg
      361 cttgacaagc atttgaagcg tgctgcaatg ccaatcatgg aagtcccccg gtcgttgaca
      421 cctttgatca cgctcgatag tataacccgg gtctatcaag aggctatcgc tgatgaacag
      481 gaagatgctt ttgccgccct tgggcctctt gctggtcgcc ggaatgattc tggcggccgc
      541 cgcgccggcg cgtgcggctg ttctaccttt gaaggtgtcg gccgacaatc ttgacgaagc
      601 cacgacgggc gaacttacga aagcgatagc cgacaagttt gccagatacc cgtcgctgac
      661 acttctgccg atgcccgagg cggatgcgat cgacgtcatg atagacgccg gttgcgagga
      721 tctgaacacc gaatgccttg ttttgattgg aaaacagtat ggcgcgacgg ccgtagtcta
      781 ttcaaccgcg gttgggatgg acgaaggcat cggactgtcg atcagatacg tcgaagtgga
      841 gtctggaaaa cagcgtgtcc aggatcgtgc ggccgcctcc ggtgtcggac cttcggacct
      901 tatgaaactg gctcttggcg aaattcttgg gcctgagcct tctaccgagc ctgccggcgt
      961 tctggtgaat ttcgtttcat tccccccggg tggcgaggtc tacgttgggc ccgaattcgt
     1021 ggggatagca ccggtttcaa ccagactctc gccgggttcc cacgacatca ggatggttaa
     1081 ggaaggatac cggacgcgtg acggcaggat cgaggtggcg actgacaaag aggccgaagt
     1141 gaagtggtcg ctctcggcga tcccggctca gccggtcgtc gctgcgccgg ttgccgcacc
     1201 gactccggca ccgattccgg catcgactcc ggccctgaca ccagcatcga ctccagccga
     1261 gccgactccg gttgcgtcgg ctcccgcgac ggcggcgact tcgacttcgg cgccggcttc
     1321 ggctccgaca accgcgacaa cgccggcaac cgtcgcaaca acgcctgcaa ccgtggcatc
     1381 aaaacccgca accgtggctt cgatcccgtc cgatcagccc gcggtcatcg atgatgtgcc
     1441 gacgattcct ccggaagagc agtcgtccaa tgagaagtcc agtgtctcga agcagccgtt
     1501 ttacaagacc tggtggttct ggaccatcat cggggtcgct gtcgtcggcg ccggaaccgc
     1561 tgccggcgtt ctggcaaccc agggcgagcc ggccggtggc agcatcggtt tttcgcccga
     1621 tccgggattt gctccgctcg atgtgacgat cttccgttga agacgggttt tctgggggtc
     1681 tgattatgat ggtcaggttt ttcgccgttt cactgattat gccggcgctt ttattggcgg
     1741 ggtgcgcggg ttcggtcgac tgcgccgggg gcacctgttt ctacgggccg tccgatcagc
     1801 agggtgcggt ttcgtttgcg ctttccgggt ccgacacgaa tgtgcgctcg ttcataatca
     1861 agctttacaa gggcgccacc gagcgggcgc gtgacaacgc gtatttccag tcgtcatgtt
     1921 ccccgctcag caagggattc acgatcagcc atgtggatgt tggcgacgat tacgtcgtgg
     1981 aatacctggg atactccgat ggagcctgca ccccgacgac tctcgtcaag caaggagtcc
     2041 gcggcggggt ccggatcacc accctgggaa cagggttctc gtactacttc attcaggtca
     2101 acactgtcgg tgacttcaca gccatgccga caccggatca gaccctgaat cccagtaccg
     2161 gtggggtctc ctgccagtca gattcagact gccgcgccgt tattccctgc cccgaacctt
     2221 ccaagtgcag gttcaaggtg aacgttgact gcacctcggc cgagatcgaa gccggggagt
     2281 gcccggacgg cgtgaaggtc atgcagtaca acgtccatcc cagagcggtc tgcgatgtcg
     2341 atacctgcag gctgaagacg ttgttcccgc tgaacaccag gggagacagg gccttcgcgt
     2401 caacagtaag aaccggttcg ggcgatgtcg tttcgttcgg tgggtttact ggcaaacgtg
     2461 acggctttta cgttgtcgag ggcgcatcag acccggcctc cgccccggaa acccagatgt
     2521 tttcaggcga cagtggaatc ttcggcgttc tcgaacatga caagccgctc gattccggcg
     2581 taggcatgtc atcgtcggtg ctgctggacg acggccgttt tgtcgttgtc ggcggaacaa
     2641 gcaaggcact tgccgccacc ggatccgatt tcgttggcgc tcccgaactt gagtccatct
     2701 tctgcaccag cgactgcccg gtccagatgt cgtcgtacct ttatgtgatc gactcccaga
     2761 ccggcgaggt cgacagaacc cagcttgccg aggcggtcat gcccgcggcc gtgttgcagg
     2821 tggcgcgtcc gacaccgtcg gtttatgtcc gtgccgccgc ttccatgaaa gactttgccg
     2881 gcgatcgcgc ttccggggcg tcgacgatat cctggctttg tcaggtatcg gattcgaacg
     2941 cggtgtcatg ttccgacgta ggaccgtcga atcccgtttc gcgtttcatg cccgcgagcg
     3001 tctgcatcaa acgttcggcg gggttgtgca gcgagctgct gaccctgggc ggaaccgaca
     3061 atatcagtaa attcgccgag atctattacg cggacaccaa ccaggtgaag gtctttccgg
     3121 gcacgggcga cgtgccggcc tatctgactg gcgccgttgc gttcatggcc ggggatcgta
     3181 tctggctggt tggcggaacc gatgatccgg acagcacgcg caccgcacgg ccttacgagg
     3241 tttcgtttga acgggatctg ggcgtcgtcg tcctgtccga agtcgaattg ccgctgtccc
     3301 aggccgatgc gcttggccga atccatcatc aggtcacggt ccttggcgac gacgaccatg
     3361 tgctggttac cggcggcatg aatcaggacg gcgtggtctc ttccggcgcg gttctgctga
     3421 agttcgatgc cggtcgaatg gatgtcgtgg atgactccct gtcgatgacg acggccagaa
     3481 tggggcattc cgcgactctt atcggcggcg gtctctacga cggaagtgtc ctgatcaccg
     3541 gggggatctc cacggccagt ggcgacttcg ccgatggggc cgaggtttat gtgccggcct
     3601 ttcagggcac cgggcattaa gtgagactca ggcttccgtt aaaggttctg ctgcctcttg
     3661 ccgccatgat cgcgctcgcg gtctgtcttc tcgttttcaa cacggttttc gctttgcttc
     3721 tgattgtgct ggtgaccgtt ttcacgctga tcgggggcgc gacggtccgc cacgcggcat
     3781 tggctgattc gggtgtcaac ggcgctccca ggttgcttgt caagggtacc gatcggttcg
     3841 tactcacgtg ggttctggtc acggcgctgc tctggacgtt ctgcgcgccc gacgtgtatt
     3901 tcagggacgc cggcgagttg accggcgccg ccgtcaatct cggcgtgccg catccgaccg
     3961 gctttccact gttgtgcatg cctggaaagc tgttttcgct ttttcccgca ggcaacgtct
     4021 acttccggtt caacctgctt tccggtttcg cggccggagg cgccgccgcc tttttgcccc
     4081 ttttcttcga gcgcgtattc gcgtccgacc gctccgatca gaccggcgtc tggtgggtcg
     4141 ggccactgat ctatctggga tcggccgcgg catggctgca cggtaccaca cccgaaatct
     4201 acagcatttc cctgttcggc atgctcgtaa ccatctttct ggcgctggag gcggcaaggg
     4261 cgaatgacgc gcgactgctg gtagcgtccg cggtgctttc cggcctcggc ctcggtggtc
     4321 atgtgacgtg gcccctgtat tccctgccgg tcgtcgtggc ggctctggcc gttttctcat
     4381 tccgggccgc gtccggtcag cgtgcgcgca cctttttccg ggtgggcttt ctgtcgctgt
     4441 tcgcctttgt catcggcgcc atggtcgtgt tctacctggt ttcggccgcc tcgcgcgatc
     4501 cgttgatgaa ctggggagac ccttcgacct tcaaaggtct tcttgctcat ctctccgggc
     4561 agcggattcg cgattccttc gcgggtcgga tgacttcact gaatcccgcg gtgctgctcg
     4621 tgaacctgtc actggcgatc agatcgctgt gggattcgct tggtcctgtc ctgccattcg
     4681 cattagtcgg tgcgctgcac gccatcctca ggcggtcgtg gtggaccact tccgtattgg
     4741 tcctggtcct ggccgatctt gtgttcgccg tgttcatcaa tcccatggga atcagggatc
     4801 ttcaggtgct tctgacggcc acatggggac tggctttgct ggcggcgtca ggcgcgtcac
     4861 tggcggtggc cttccttggc cgcaggggtg gggcgccagg tgctgtcgcc atgatcgggc
     4921 tggtctgtgt ttcactggtc tttcaatggg ttacctcacc cgcggaccgg gacatgcgca
     4981 atctgtacgg tgggcggatc atctcggaca tcctgctgga caaggccggg ccgggggccg
     5041 ttgccatgac cgcgtcggac gacatgtcgg ctcttttcat ggcaaggacc gcggtcg
//
LOCUS       DAOPPA010000214         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17861_length_5094_cov_3.850764, whole genome shotgun sequence.
ACCESSION   DAOPPA010000214 DAOPPA010000000
VERSION     DAOPPA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17861_length_5094_cov_3.850764"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH54_07500"
     CDS             <1..224
                     /locus_tag="PKH54_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99774.1"
                     /translation="TYVDSNGNEATVDEYSAVPYSRNVKIYQINYSLNCKILDERLLE
                     RVPERIHVQIDTKDIAGKGSWEFRWVMTD"
     gene            350..793
                     /locus_tag="PKH54_07505"
     CDS             350..793
                     /locus_tag="PKH54_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99775.1"
                     /translation="MLKSTVVVFGLIAVICMGCGATIRSSQEGMVRQHLIVTNVGGDP
                     VPGTSVYVNDKHVGDTNEFGWITHEIPGPHKGDSFKLKVNKSGYQAISRDVLAVVDPG
                     FLTGDVFLGLIFGFIPIMFTLGVDIVTESVFAYPRSLRIVLERKN"
     gene            complement(885..2303)
                     /gene="lpdA"
                     /locus_tag="PKH54_07510"
     CDS             complement(885..2303)
                     /gene="lpdA"
                     /locus_tag="PKH54_07510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC99776.1"
                     /translation="MVRHDLVVIGAGPGGYVAALRAAQLGMNVACVEREEMLGGTCLR
                     VGCIPSKALLESSERYHTTVRDLEDHGVKVSGVELDVAALMKRKDKIVKGLTGGIAML
                     FKKAGVTRYAGQARLCTIADAAPGCAGAAVVVTAADGTQTEVRADRILIATGSMPSAL
                     RGIEFDNDRIGTSTQALSWPEVPAELIIIGAGIIGLELGSVWSRLGAKVTVLEYLPRI
                     LPGIDADIADKAYKVFRRQGLKFRLETRVESARFNGEKCEVRITGESEPLLADRVLVA
                     TGRRPETEGLGLEDAGVQVDGRGFILVNQDYLTSADGIFAIGDVIGGLMLAHKASEEA
                     VACVERMNGHDAHVNYGVIPGICYTDPEVAGVGRTEDELVAAGVSFRKGVYLYRANGR
                     AQSIARTDGEVKILADAETDKILGVHILGAHAGELIAEAAIAMVGGMTVTALADACHA
                     HPTLSEILKEAALACCGKPLHA"
     gene            complement(2355..2873)
                     /locus_tag="PKH54_07515"
     CDS             complement(2355..2873)
                     /locus_tag="PKH54_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99777.1"
                     /translation="MKRILLAISMISVWAMAACGAKPAAEVQPAQVASPQVQAEQAAP
                     VEPPQAPAAQVDPAAQAATGGTQPAGDPTGAVPAAPAGEKKYQGEPGRQFIVTDLHVG
                     PNRAIDAGDIKIKNPDALKLRVSPNSEIAPPGNPAGNDVQTPTTSGLRRPPFKPRAND
                     GTAPAQGQAQGN"
     gene            complement(3066..3995)
                     /locus_tag="PKH54_07520"
     CDS             complement(3066..3995)
                     /locus_tag="PKH54_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC99778.1"
                     /translation="MTLQSIPGIGEFFSATSAILWALSLVLFRRAGDSMAPSTLNLYK
                     GTVALACLIPTMALLGIDYFPDDVTIQDWLIMLASGMAGMGIADTILFVSLNKVGPGR
                     YAIINCSFGPATILASAVFLGEPVTAALIGAAALIVAAILLSAWDPVGRMQAKAATPD
                     PNRPRLGRYYLLGVLGVVIIAVSIVAAKPVLNRTDGVWGAFARLCGGLLFLVPQNLMP
                     GNFKRTVAAFSRGPHHRHALLPSFLGAYACTVLWTLGMKYTYASVASVLCQLTNILLF
                     PFAFLILKDRIQARQIVAVGIGIGAGLLIILAN"
     gene            4118..>5094
                     /locus_tag="PKH54_07525"
     CDS             4118..>5094
                     /locus_tag="PKH54_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99779.1"
                     /translation="MKCLMVKTVRAILAVSATFLVFSASGCGDGADPAGDATIDSMTD
                     LQDPDVAPDEGSPDVVGDTGPDTSGDQGQQDQIGETVSDVPACDPGADCSAGPDNVVL
                     QAGSNKLEFDRVARTITLKYGDQPRLILDFDAIQVGAVPALDPTMTYDPYPMLARDEG
                     FYPQSGLKWLPLTHFVHKITDGCTSSFDVTMSDGINSVMGKMVVTAPEDGSFGVNLIP
                     MDGDMEVAYFRIAPDVTETENFYGLGAYLDQVAHRGKVRAMQLETDFNIESSYNEAHV
                     PVPLLISTNAWGIFVESWYPGAFDCAAWESTKVDIVFGTGEGSSDGFQF"
BASE COUNT         1023 a         1461 c         1532 g         1078 t
ORIGIN      
        1 ctacatatgt cgacagtaat ggcaatgaag cgactgtaga tgagtacagt gcggttcctt
       61 attctagaaa tgtcaaaatc tatcaaatca actattcatt gaactgcaaa atattggatg
      121 aacgactgct cgaaagagtt cccgaacgca ttcatgttca gatcgataca aaagacatcg
      181 ctgggaaagg gtcttgggaa tttcgatggg ttatgaccga ttaaatctga gtgccaccgg
      241 gattcgagtg ttaccttttc gtaatacttc agtaatacgt aaaatgcagt gtggttcttc
      301 tgtctttgaa caaggccaag cctacttaag gtacatgggg tattaagcga tgctgaagtc
      361 aactgttgtc gtgttcggtc ttatcgctgt tatatgcatg ggttgcggcg ctacaattcg
      421 cagttcgcaa gaaggcatgg ttagacagca tctcattgtg acgaatgtcg ggggtgatcc
      481 tgttcccggc accagcgttt acgtcaacga caaacatgtc ggcgacacga atgaatttgg
      541 ctggatcacg catgagattc ccgggccgca taaaggcgac tctttcaagc tgaaggttaa
      601 taagtcggga tatcaggcga taagtcggga tgtgctcgcc gttgttgatc ccggatttct
      661 gacaggcgat gttttcctgg ggcttatctt tggattcatt ccgataatgt tcaccctggg
      721 agttgacatc gtaacggagt cagtgttcgc ctatcccagg tcgttgagaa tagttcttga
      781 aagaaagaat tagtcctacc gtatctccat attcactttg agcaacggaa tttctgcggg
      841 tagattcgag tcgtcggcgc gaggcgggag gtggttgggt cggttcatgc gtgcaggggt
      901 ttgccgcagc aggcgagggc ggcttccttc aggatttccg agagggtcgg gtgggcgtgg
      961 caggcgtccg ccagggcggt gacggtcatg ccgccgacca tcgcgatggc ggcctcggcg
     1021 ataagctcgc ccgcatgggc gccaaggatg tgaaccccaa gaatcttgtc ggtttccgcg
     1081 tcggccagga tcttgacctc gccatcggtt cttgcgattg actgggcgcg gccgtttgcc
     1141 cggtacagat agacgccctt gcggaaggaa acgcccgccg cgaccagttc atcttcggtc
     1201 ctgccgacgc cggcgacctc gggatcggtg tagcagatgc cgggaatcac gccgtaattg
     1261 acgtgcgcgt catgcccgtt catgcgctcg acgcaggcca cggcttcctc tgatgccttg
     1321 tgggcaagca tcagtccgcc gatgacatcc ccaatggcga agatgccgtc cgccgatgtc
     1381 aggtagtcct ggttgaccag gatgaagcct cggccgtcaa cctggacccc ggcatcttca
     1441 aggccaagcc cttcggtttc aggccgcctc cctgtcgcca ccaggacacg gtccgccaga
     1501 agcggttcgg attcgcccgt gatgcggact tcgcattttt ccccgttgaa ccgggccgat
     1561 tcaacgcgcg tctccagacg gaacttcaat ccctggcgcc ggaatacctt gtacgccttg
     1621 tcggcgatgt ctgcatcgat gcccggcagg attctcggca ggtactcgag gaccgtgacc
     1681 ttcgcgccaa gccgggacca gacgctgcca agctcgagac cgatgatgcc tgcgccgatg
     1741 attatcagct cggccgggac ttcaggccac gacagtgcct gggtgctggt tccgatccgg
     1801 tcgttgtcga actcgatccc gcgaagggcg gacggcatgc tgccggtcgc gataaggatg
     1861 cgatcggcgc gaacttccgt ctgcgtgccg tcggcggccg tcaccacgac ggctgcgccg
     1921 gcgcagcccg gggcagcatc ggcgatcgtg caaagcctgg cctggccggc ataccgggtg
     1981 accccggcct tcttgaacag catggcgatt ccgccggtca gtcccttgac gatcttgtcc
     2041 tttcgtttca tcagggcggc gacgtcaagc tcgacgcccg agaccttgac cccgtgatcc
     2101 tcaaggtcgc gaaccgtcgt gtgatagcgt tccgacgact ccagcagtgc cttgctgggg
     2161 atgcacccga cccgcaggca agtgccgcca agcatttcct cgcgctccac gcatgccacg
     2221 ttcattccaa gctgggccgc gcgcaatgcg gcgacgtaac cgccgggacc ggcgccgatg
     2281 accaccagat catgtctgac cacgttcacg ttgcacctgt gattaattgg tttgttaact
     2341 gggttcccgt cgcgtcagtt accctgtgcc tggccctgtg ccggggcagt cccgtcgttg
     2401 gcccgcggct tgaacggcgg gcggcgcaga ccggacgtgg ttggcgtctg gacatcgttc
     2461 ccggcaggat tcccgggtgg cgcgatttca ctgtttgggg aaacacgcag tttcagtgcg
     2521 tcggggttct tgatcttgat gtcgccggcg tcgatggccc ggttggggcc gacatgcagg
     2581 tcggtgacga tgaactgacg gcctggttcg ccctggtact tcttttcacc agccggagct
     2641 gcaggcacag cgcccgtcgg atcgccggcc ggctgcgtac cgcccgtcgc cgcctgggcg
     2701 gccggatcga cctgtgccgc tggcgcctgg ggcggctcca ccggtgccgc ctgctctgcc
     2761 tgaacctgtg gtgatgcaac ctgtgccggc tgaacctcgg cggcgggctt cgcgccacaa
     2821 gcagccatgg cccagacgga aatcatcgaa atcgcaagca ggatcctttt catgaatgct
     2881 tctccaagag catcgacggc agaattatcc accagatggc atggatagac aaccgtccgg
     2941 tggaagggca tcgatggcgg ggagaatgat tccacgaccg gatcatggca atccgcgacc
     3001 gggtcatggc aatccgcgac cgggtcatgg cacgaaatcc cttcgcgatt aacagacttt
     3061 gatgctcagt ttgccaggat gataaggagt ccggcgccaa tcccgattcc aaccgccaca
     3121 atctgcctgg cctgaatcct gtccttcagg ataaggaacg cgaacgggaa cagaagaatg
     3181 ttggtcaact ggcaaaggac gctggccacc gatgcgtatg tgtatttcat ccccagggtc
     3241 cacagcacgg tgcacgcata cgcccccagg aacgacggaa gaagggcgtg ccggtggtgc
     3301 ggcccccgcg aaaaggctgc gacggtccgt ttgaagtttc ccggcatcag gttttgcgga
     3361 accaggaaca gaaggccgcc gcaaagcctg gcgaaagcgc cccacacgcc gtcggtgcga
     3421 ttcaggaccg gcttggcggc aacgatcgat acggcgatga tgacaacacc aagcaccccg
     3481 agaaggtaat aacgcccaag gcgcgggcga ttcggatccg gcgtggccgc ctttgcctgc
     3541 atgcgtccca ccggatccca tgccgaaagg agaatcgccg ccacaatcag cgccgccgcg
     3601 ccaatcagcg ccgcggttac cggttcccca agaaacaccg ccgaagcaag gatcgtcgcg
     3661 gggccgaatg aacagttgat gattgcatac ctgcccggcc cgaccttgtt caacgaaacg
     3721 aaaaggatcg tgtcggcgat ccccatgccg gccatgccgg atgccagcat gatgagccag
     3781 tcctgaatcg tcacgtcatc ggggaaataa tcgatcccca gcagtgccat tgtcggtatc
     3841 aggcacgcca gcgcaacagt gcccttgtaa aggttcaagg tcgagggcgc catcgaatcg
     3901 ccggcccgtc tgaacagaac cagcgaaagt gcccacagaa tcgcggaagt cgccgaaaaa
     3961 aattccccaa tgccgggtat cgactgaagt gtcatgtttt tcgttttcct gttgcgccgt
     4021 cgcgcacggt ttgcgcgcga taagctagca cgtaagcgtt acaactgata gtattcgtcg
     4081 tcttgtgatc ccaggccatg gcaggaggtt catactgatg aagtgcttga tggtaaagac
     4141 ggttcgggcg attttggcgg tttctgccac ttttttggtt ttttcggctt cgggttgcgg
     4201 tgatggagcc gacccggcgg gcgacgcgac aattgattcg atgaccgatc ttcaggatcc
     4261 cgacgtggcc cctgatgagg gctcccccga tgtcgtgggg gataccggcc cggatacatc
     4321 gggcgatcag ggccagcagg atcagattgg ggaaaccgta tcggacgtgc cagcctgcga
     4381 cccgggcgcg gattgttcgg ccggcccgga taacgtggtg ctgcaggctg gatcgaacaa
     4441 acttgagttc gaccgcgtgg cccgcaccat taccttgaag tatggcgatc agccacggct
     4501 gattcttgac ttcgatgcca tccaggtcgg cgccgttcca gcgcttgacc cgacgatgac
     4561 ttatgatccc tatccgatgc ttgcccgcga cgaaggcttc tatccgcagt ctggcctgaa
     4621 atggctgcca cttacgcact tcgttcataa gataaccgac ggttgcacgt catcatttga
     4681 cgtaaccatg tccgacggca tcaattctgt catggggaag atggttgtca cggcgcccga
     4741 ggatggcagc tttggcgtta atctgatccc gatggacggt gacatggaag tcgcttattt
     4801 ccgtatcgca ccggatgtga ccgagaccga gaacttctac ggccttggcg cctatctgga
     4861 tcaggtggcc caccgcggaa aggttcgcgc catgcagctt gagaccgact tcaacatcga
     4921 aagcagctat aacgaggcgc atgtgccggt tcccctgctt atcagcacca acgcgtgggg
     4981 aattttcgtg gaatcctggt acccgggggc gtttgactgc gccgcatggg aatcgacaaa
     5041 ggtcgatatc gtgttcggga ccggggaagg cagttccgac ggtttccagt tcca
//
LOCUS       DAOPPA010000215         5088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17891_length_5088_cov_3.887542, whole genome shotgun sequence.
ACCESSION   DAOPPA010000215 DAOPPA010000000
VERSION     DAOPPA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17891_length_5088_cov_3.887542"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1735)
                     /locus_tag="PKH54_07530"
     CDS             complement(203..1735)
                     /locus_tag="PKH54_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99780.1"
                     /translation="MKVVSSVFVLLAVSLILAPALAQEAEPGAGVGDSVTVEGTTGEA
                     GDAASAPSSASAAGDGVDFVGDRPPEADEPGQRAAEGDASGAEVADEQAADGEPASAD
                     EGSSEEGEPVEDESAKSEDRKEKESRIDKEAKKLKPFQGAHMMVGRNVKMPTATKRFA
                     IGIELSVSPLNVIGRLARDQFIDAAYNQVCTATPNPDQCRESVEKAVDVLGEMSDSEW
                     ELVRGVASGDKASLIQALSDAGVSDPQVQATVANVVDQYAGQYLEGDAYAQAVDAIRA
                     GSSASATNMLFNAYLQMNFKWIEATVFAPFAIRNSSEGTEADMANISVDLKAGGDWSF
                     GKVAALAVAGGIELYMPSGTSGITGSRGTNAFYTPKFTYGMLTWAPYLVLGVELARII
                     QLQGHVELVSMHGVLDGAASSLEMYLKYGGGFILLPRFIVSIIFELNGITGLKNSTWD
                     EVLTVGAGINISFKIFKIFLGVSMPLLDQGRYVWEPGYWSGANYTQLSRFGVTSRIGF
                     EF"
     gene            complement(1856..2767)
                     /locus_tag="PKH54_07535"
     CDS             complement(1856..2767)
                     /locus_tag="PKH54_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99781.1"
                     /translation="MKKVALAIGIVTALVIAGCGGDGSDDTGNGNDVFIYVDYGDGDD
                     TSRPDIQIPPDLVVYDDEGNPIDLVDYDYGHLIDTVQPKDNGTVKDTANPPDTTGEDA
                     NISDFCTPPTSCAFNTINDMRDNCDGTFTDLKTGLMWQGLGEDHPLAATNSNLMSRQC
                     NTTKTGCNNGRYYEDWRMPTIEEVRSLVRGCPATAAGGSCPASSSCRAFDECLTDQCN
                     GCGTGGDFEYKDGEDTYYKYIDPRMKSVAYGSSYSAVMSSTSVNKTTSDARYRFFYLL
                     NYNAKLAALNPMQQATDGVMFCVRAGN"
     gene            complement(2877..>5088)
                     /locus_tag="PKH54_07540"
     CDS             complement(2877..>5088)
                     /locus_tag="PKH54_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99782.1"
                     /translation="VHDAAVGTESFAQTVAGIAALVECGIAVDIDVPVCSFNVGGLSG
                     IGRLAARLGARSVCFRLPPVATSGGIERIPDLKAACDAVTSAVLDMDDGFPEVTSGYC
                     DLPCCLLPALKGRELQGARTADTIIVNADGIPELIPSPDPSRAMPVPCAACELRRSCP
                     TIEPDLVRERGAGDLHLPLQSVSDAVFTRVRRMVEPLIVDGRCPWRDGPIQSVLPVDY
                     LHLVTPAGWELWTLSAGHWDSSTLMHAKNIRNNLVMNSSGRIPSLDGLCANCENLARC
                     AGVFHVGPDSPVAAMPSASISSAKAGTADFLRWPDFASGFSDFVRSSAGSPVSINGTA
                     PVIRLLDEPSGPASSFLPQVEPLMLWEAARKATMCGLTDYDLPYVQPAFRLEIQASDR
                     WAEVDRMGTILIIRKCTMNCIICQVQKFYEGIDIMPLPDVVKFLEEFRLLGYTRLDLF
                     GGEPTMRRDLVDLVAFAHRMGFYTDLITNGTLMDDALATALRDAGLDLCIVSLDGPTP
                     EIHDKIRRVKDGHQRAVSGIEAVVRAGGMEVNVDTVVLPDNIDYLIDLARQVSALGVT
                     RINMFLCLEGPISSPVPRLLGFERTLDFYERIVPEMRRIGEPCGTSISVGPRLPMAGR
                     SIRDVAATRMFADVCEGTYNVVFRHPEILCKAPDDEVYISLFGDVFPCTAPQMLESSA
                     RMGNVYRQKLIEVVRSEKWNEFKKISGHHEGCRMCWRAHFDLDREGEERILDSN"
BASE COUNT         1063 a         1564 c         1469 g          992 t
ORIGIN      
        1 tgccgcgggc accagggaaa taagcgccag cgtcgcgcac agcgtcacca gtgagcccac
       61 ttgccacttg attgacatga ccgcctccag aaaaagggaa aaccattgga atcaatgcaa
      121 gcgctattgt cgcgcccgtt tgccgattac gtcaaacccg tgaaatgtaa atgcttgtaa
      181 ggaagccgat tccgggcacg ggctagaact cgaagccgat cctggacgtc accccgaacc
      241 ttgaaagctg ggtgtaattg gctccgctcc agtacccggg ttcccagacg tacctgccct
      301 ggtcgagaag cggcatgctg acgccaagga agatcttgaa aatcttgaag ctgatgttga
      361 ttccggcccc gaccgtcagg acttcgtccc acgtcgaatt cttcagcccg gtgatcccgt
      421 tcagttcgaa aatgatgctg acgatgaacc ttggaagcag gatgaaacca ccaccatact
      481 tgagatacat ttccaggctg ctggcggccc cgtcaagaac cccatgcatt gaaaccagtt
      541 ccacatgccc ctgaagctgg atgatcctcg caagctcgac cccaagaacc agatatgggg
      601 cccacgtaag cattccgtag gtgaatttgg gcgtgtagaa cgcgttcgtg ccgcgggaac
      661 cggtgatccc ggaagtcccg ctcggcatgt aaagctcgat gccgcctgca accgcaaggg
      721 cggcgacctt cccgaacgac cagtcgccgc cggccttcaa atcgacagat atgttcgcca
      781 tgtccgcctc ggtgccctcc gaactgttcc tgatcgcgaa cggggcaaag acggtggcct
      841 cgatccattt gaaattcatc tgcaggtatg cattgaacag catgttcgtg gcgctggccg
      901 aagacccggc tcgaatcgcg tcaaccgcct gggcataggc atcgccttca aggtactggc
      961 ccgcgtactg gtcaacgacg ttggcgacgg tcgcctggac ctgcggatca ctgacgccgg
     1021 cgtccgaaag cgcctggatc agcgacgcct tgtcgccgga agcgaccccg cggaccagtt
     1081 cccattcgga atcggacatc tcgccaagca cgtcaaccgc cttttcgacc gactcacggc
     1141 actgatccgg gttgggcgtc gccgtgcaca cctggttgta cgcggcatcg atgaattgat
     1201 cgcgggccag tcgcccgatg acgttcagcg gtgaaaccga aagctcgatc ccgattgcga
     1261 agcgtttcgt ggcagtcggc atcttgacgt tgcggccgac catcatgtgg gcgccctgga
     1321 atggcttcag cttctttgcc tccttgtcga tccgggactc cttctccttt ctgtcctccg
     1381 acttcgccga ttcgtcctcc accggctcgc cttcctcgct ggagccttca tcagccgaag
     1441 cgggttcccc gtccgcagcc tgttcgtcgg ccacttccgc gccggacgca tccccttcgg
     1501 cggccctctg acccggttca tcggcctccg gcggccgatc gcccacaaaa tcgacgccat
     1561 ctcccgcggc cgatgccgaa gacggagcgg acgccgcgtc cccggcttcc ccggtcgtgc
     1621 cttcgaccgt aaccgaatcg ccgacgcccg cgccgggctc ggcctcctgc gccagggccg
     1681 gcgccaggat caacgagacg gccagaagta cgaaaacaga tgaaacgact ttcaagcggg
     1741 cttccgtttg cggcgcgatc agcatcgggc acctcggttt caaattcgtt tcattttatg
     1801 tttgccggat ggatttatcc agaaacggga tcgggaaggg acgggaagag tggtctcagt
     1861 tgccggcgcg cacgcagaac atgacgccat cggtagcctg ctgcatcggg ttcagcgcgg
     1921 ccagcttggc gttgtagttc agaagataga agaagcggta ccgggcgtcg gacgtggttt
     1981 tgttgacact ggtcgatgac atcaccgccg aatacgacga accgtaggcg acactcttca
     2041 tgcggggatc gatgtacttg taataagtgt cctcgccatc cttgtactcg aagtcaccac
     2101 cggttccgca accgttgcac tgatcggtca ggcattcgtc aaaggcgcgg cacgacgatg
     2161 aagcggggca ggagccgccc gccgcggtcg ccggacagcc gcgcaccagg gaacggactt
     2221 cctcgatggt cggcatgcgc cagtcttcgt agtagcggcc gttgttgcat ccggtctttg
     2281 tcgtgttgca ctggcgggac atcaggttcg agttggtcgc ggccagcgga tggtcttcgc
     2341 caagtccctg ccacatgagc ccggtcttca ggtcggtgaa agtgccgtcg cagttgtccc
     2401 gcatgtcgtt gatcgtgttg aaggcacagg atgttggcgg ggtgcaaaaa tccgatatat
     2461 tggcgtcctc gccggtggta tccggcgggt tggcggtatc cttgaccgtg ccgttgtcct
     2521 tcggctgcac cgtgtcaatc aggtggccgt aatcataatc gaccagatcg atcggatttc
     2581 cctcgtcgtc ataaacaacc aggtccggcg gaatctggat atccggacgg ctggtatcgt
     2641 cgccgtcgcc gtaatcaacg tagatgaaca cgtcatttcc gttgcccgtg tcgtcacttc
     2701 cgtcgccgcc gcagccggca atcaccagcg cagtcacaat cccgatcgca agagccacct
     2761 tcttcatgaa cagcaccccc gttaaaaccg ccagatttta tttgttttgt aaaaaaaatg
     2821 cgataccaag aaataacgcg cccccccggg agactgaatc tgcggcgccg aaaacgtcag
     2881 ttcgaatcaa gaatcctttc ctcgccttcc cgatccaggt cgaaatgcgc ccgccagcac
     2941 atccgacagc cttcgtgatg tccgcttatc ttcttgaact cattccattt ttccgatctg
     3001 accacttcaa tcaacttctg acgatagacg ttccccatcc tggcgctgga ctcaagcatc
     3061 tgcggcgcgg tgcacgggaa gacgtcgccg aacagggaga tgtaaacctc gtcgtccggc
     3121 gccttgcaaa ggatttccgg atgccggaag acgacgttgt aagttccctc gcagacgtcc
     3181 gcaaacatcc ttgttgccgc gacgtcgcga atcgatcgtc cggccattgg aagccttggg
     3241 ccaaccgaaa tcgacgtgcc gcagggctcc ccgatccggc gcatttccgg aacgatccgc
     3301 tcatagaaat cgagtgttcg ttcaaatccg agaagacgcg gaacaggcga actgattggt
     3361 ccctcgaggc acaggaacat gttgatcctt gtaacgccca gggcactgac ctgcctggcc
     3421 agatcaatca ggtaatcgat attgtccgga agcacgacgg tatcgacgtt gacttccatg
     3481 ccgcctgccc tgaccaccgc ctcgatgccg cttacggccc gctggtgtcc gtccttgacc
     3541 ctgcggattt tgtcgtgaat ttccggggtc gggccatcca gggaaacgat gcacagatcg
     3601 agtcccgcat cgcgaagggc cgtcgcaagg gcgtcgtcca tcagcgttcc gttggtgatc
     3661 aggtcggtgt aaaagcccat tcgatgggcg aatgccacca gatccaccag gtcacggcgc
     3721 atggtcggtt cgccgccgaa cagatccagc ctggtgtatc caagcagccg gaattcctcg
     3781 aggaacttta cgacgtcggg aagcggcatg atgtcgattc cctcgtagaa cttctgaacc
     3841 tggcagatga tgcagttcat ggtgcacttc ctgatgatca ggatcgtgcc catgcgatct
     3901 acctcggccc accgatcgga agcctgtatt tccaggcgga atgccggctg aacgtacggc
     3961 aggtcataat ccgtcagacc gcacattgtg gcctttcgag cagcctccca caacatcagg
     4021 ggttcgacct gcggaagaaa cgatgacgcc gggcctgatg gttcatccag cagcctgatg
     4081 accggagcag tcccgttgat ggagacgggg gagcctgccg aagaccgaac aaagtcggaa
     4141 aagcccgacg cgaaatccgg ccaccggagg aagtctgccg ttccggcttt cgccgaagag
     4201 atagaggccg aaggcatggc ggccaccggg gaatccggcc caacgtgaaa cacccccgcg
     4261 cagcgggcaa ggttttcgca gttggcgcaa agaccgtcaa gcgacggaat gcggccggat
     4321 gaattcatca ccaggttgtt gcggatgttc ttcgcatgca tcagggttga actgtcccag
     4381 tgtcccgccg acaaagtcca gagttcccat cccgccgggg tcaccagatg aagataatcg
     4441 accggaagga cggactggat cggtccatcc cgccaggggc agcgaccatc gacgatcaaa
     4501 ggttcgacca tccttcgaac ccgggtgaac acggcatcgg aaaccgactg gagaggaaga
     4561 tgaagatcgc cggcccccct ctctcgaacc aggtcgggct ctatcgtcgg acaggagcgg
     4621 cgaagctcgc atgccgcgca cggaacgggc atggcccggg aaggatcggg tgaaggaatc
     4681 aattccggaa tgccgtcggc attcacgatt atcgtgtcgg cggttcgcgc gccctgcagt
     4741 tcgcggccct tcagggccgg aagaaggcag catggcagat cgcaatatcc gcttgttact
     4801 tccgggaacc cgtcatccat gtcaagaacg gcggaagtga ccgcgtcaca ggccgctttc
     4861 agatccggaa tgcgttcaat cccgccggag gtcgctaccg gaggaaggcg gaaacagacg
     4921 cttcgggcgc cgagacgggc ggcaaggcgt ccgatgccgg aaagcccgcc cacattgaag
     4981 gaacagacgg gcacgtcgat atcaaccgcg atcccgcact cgaccagcgc cgctatgccg
     5041 gcaacagtct gggcgaatga ttcggttccg accgcggcat cgtgaacg
//
LOCUS       DAOPPA010000216         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17900_length_5086_cov_3.739614, whole genome shotgun sequence.
ACCESSION   DAOPPA010000216 DAOPPA010000000
VERSION     DAOPPA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17900_length_5086_cov_3.739614"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKH54_07545"
     CDS             <1..506
                     /locus_tag="PKH54_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99783.1"
                     /translation="KMDGKTTETFRVLVRKDTFKTMDELKGKKISGTPLAEPAFVEKV
                     VLAGKYPLSSFVAEKARGSIKALRDLTGSKIDAVLVTEQQFNASKSLDWARDLEVIFT
                     SKALPIMPLVASKAKTTAKDREGFIRTMERFCDDKEGKQFCKLFGISGFVKADNALLN
                     EARGLWK"
     gene            497..1435
                     /locus_tag="PKH54_07550"
     CDS             497..1435
                     /locus_tag="PKH54_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99784.1"
                     /translation="MEIAPGRHAVLFIFSVTVLFVAGCSPAYKGNLFKLDLPDDCPGN
                     EEALTRDRTDPLTAGQDQLRCALQAVRNMQPPERQPRAHEALTASRICSLLAESSPAG
                     AAGDERRKSLAWEGVAWAEYSMGTGLAIDPATSYYYSINLGIAVRDSILTAMKYLGRL
                     QRHLGRAVREAPELDSGGPLRTMGYLLIKAPPWPAGIGDPEKGIEMLRKAVEQFPDYP
                     PNRIYLARGLWEADEDEDSALEIAKAAADSLRAGSWGIRHQAWIRDLSDLLNQIGNQD
                     AADEILSGLDSDSTAPTGTDAANQAGGNSGLSPSVP"
     gene            1410..3140
                     /locus_tag="PKH54_07555"
     CDS             1410..3140
                     /locus_tag="PKH54_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99785.1"
                     /translation="MASPRRYRNIALALSATFIIAQAWICADPLAGTHDSSSSFLGFR
                     HLPTDHFAYMMYSTQTARDGSLLTKNLFTTARQDGRFLMVGLVIAGAVRGITGLPDPV
                     VWHGLRLLVLAAFCLVLWKLVQAVFKEGRKAVAAHAFVLFAGGLDWLVRPFFRDRMAS
                     TGLDWVNFIDNPWNFSVFWASTNLVWAIPMTLITLMILLEIRWLEQAAVKNADRSAEL
                     ARIRAQTGDHRVGSMDTVSKPADFLPGVVRGMVFAALWFIHPYSAIVWGLLVAVAVVV
                     PPIGTSLKRSLFINLPALAGPALVAVFITWSQQDPVVAASNAQTGLWKLGYPPIIYPI
                     VYGPWMLLPLGLVMRRNTIDRHVMRWLLLWFVTGFAMMANPFVTGVKFQFSFLVPFLI
                     LQIAGLFALVQRRQAAGGRTSWPGGAIAAFVVLASLNSAAGLVLDVNPTATRTAARAG
                     KDHLALLKELETLPDGGVLCDPFDAQIVPWKAGKPVFVGQWFLSTRYQEKAALVRWFY
                     SDEPDEARTAGFLNNAGIRWILYGPREMQLGKMPRADGLRLVRQIAGRQIWEWAPAGA
                     EWPSILQQSK"
     gene            complement(3117..4511)
                     /locus_tag="PKH54_07560"
     CDS             complement(3117..4511)
                     /locus_tag="PKH54_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99786.1"
                     /translation="MMRPYYSIAAVFLMFLAGAAHASQNPGSDKSAKSGSSESEWGRP
                     FAELQFSGVAGGSHESWRAGFDFALSGGRDGFEVSVIAPIRFDPDGFRMREWDEPNDF
                     GRLLSRFGWTDRTGRFDVSIKPVQNFNLGMGNLVSMFNGVIDGDHFRTGVSARLHWDV
                     AGGDLFLDGVAGPSVIGGRVYFRPLWFVDRSGQIGRLEIGASFAGDINAPSNLGASTP
                     ENPTSSVPGPNGLVATDPSPLTAWSIDVSWPVRPARAIQIKPWASWSRIGWSDASHVG
                     LELAFNIRSKVVLTVTGRYDFMESGFVTGYFDNVYMADRYDFNAFRDAAEGPVSKRVV
                     TGASRPARHGGAFGMMAGWDPWLTAWIGADVDQYGAFSRIRAGLTVKVPDRLLLTGTV
                     YARGFGVDGRQRLADRIFCSVAADVTVWKYIGVFALYSRDLFVPDSGSDRFRYVSGNT
                     GLAGVRFSFGLLKD"
     gene            4646..5053
                     /locus_tag="PKH54_07565"
     CDS             4646..5053
                     /locus_tag="PKH54_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99787.1"
                     /translation="MTVSNRQMALILLFFAICAGAGYAVYWWHETSLDRIAMFHVSFK
                     GESLNRVSAALADKRPAQAREILQRQPLFEKIGILKVLSRDEAVAVRMFAVRCMRPLR
                     DVPQIRAELARLAAEDPEESVTRLASRALQGRR"
BASE COUNT         1035 a         1528 c         1549 g          974 t
ORIGIN      
        1 cgaagatgga cgggaagacg acggaaacat tccgcgtcct tgtccgcaag gacacgttca
       61 agacgatgga cgaactgaag ggaaaaaaga tcagcggcac gccgctggcc gagcccgcgt
      121 tcgtcgagaa ggtcgttctg gccgggaaat acccgctttc ctcgttcgtc gcggaaaagg
      181 cgcgcggctc gatcaaggca ctgcgcgacc tgaccggaag caagattgac gccgtgctgg
      241 tgaccgagca gcagttcaac gcgtcaaagt ctctggactg ggcacgcgac cttgaagtaa
      301 tcttcacatc gaaggccctg ccgatcatgc ctctggttgc cagcaaggca aagaccacgg
      361 cgaaggatcg ggaaggtttc atcaggacca tggaaagatt ctgcgacgac aaggaaggaa
      421 agcagttctg caagctgttt ggaatttccg gattcgtcaa agccgacaac gctctcctta
      481 acgaggcgag aggactgtgg aaatagctcc cggaaggcac gccgttctgt tcatcttctc
      541 cgtcacggtt cttttcgtcg ccggctgcag cccggcctac aaaggcaacc ttttcaaact
      601 tgatcttccg gacgattgtc ccgggaacga agaagcactg acccgtgaca ggacggatcc
      661 gttgacggca ggccaggatc agttgcgttg cgccctgcag gcagtcagga acatgcagcc
      721 gcccgaaagg cagccccggg ctcacgaggc gctgacggca tcccggattt gcagcctcct
      781 tgcggaatcc agcccggcgg gggcagccgg tgacgaacgc aggaagtcgc ttgcctggga
      841 aggcgtcgcg tgggccgaat actcgatggg aacaggactg gccatcgacc ctgcgacatc
      901 gtattactac tcgatcaacc ttggaatcgc ggttcgtgac agcattctga ccgcaatgaa
      961 gtatctggga agacttcagc gccaccttgg cagggccgtg cgcgaggcgc cggaactcga
     1021 cagcggcggg cccctgcgga cgatgggtta cctcctgatc aaggccccgc cgtggccggc
     1081 cggaatcggc gatcccgaaa agggaatcga gatgcttcgc aaggcagtcg agcagtttcc
     1141 ggattatccg ccgaaccgga tttatcttgc caggggcctc tgggaagccg acgaggacga
     1201 ggactccgcg cttgagattg cgaaagccgc ggcggattcc ctgagggccg gaagctgggg
     1261 gatcaggcac caggcctgga ttcgcgacct gagcgatctt ctgaaccaga ttggcaacca
     1321 ggatgctgcg gacgaaatac tttcgggcct cgattccgat tcgaccgcac cgaccggcac
     1381 cgatgcagca aaccaggcag ggggtaacag tggcctctcc ccgtcggtac cgtaatatcg
     1441 ccctggcgct ttcggccacg ttcatcatcg cccaggcctg gatctgcgct gacccccttg
     1501 caggaaccca cgattcaagt tcatccttcc tggggttcag gcatcttccg acggaccatt
     1561 tcgcctatat gatgtattcg acccagaccg cgcgcgacgg gtcgctgctg acaaagaatt
     1621 tgtttaccac cgccaggcag gacggacgat tcctgatggt cggcctggtt atcgcgggcg
     1681 cggtcagggg catcaccggc cttcccgacc cggttgtatg gcacggactc cggctgctgg
     1741 ttctggccgc tttctgcctg gtgctgtgga aacttgtcca ggccgtcttc aaggaaggtc
     1801 ggaaggccgt cgcggcccac gcatttgttc tctttgccgg cgggctggac tggcttgtcc
     1861 gtccattctt ccgggacagg atggcatcga ccgggctgga ctgggtcaat ttcattgaca
     1921 acccctggaa tttcagcgtg ttctgggctt cgaccaacct tgtctgggcg atcccgatga
     1981 cactcattac cctgatgatc cttctggaaa tcaggtggct tgaacaggcc gcggtgaaga
     2041 atgccgacag atcggccgag cttgccagga ttcgcgcgca gaccggggat catcgagtcg
     2101 gaagcatgga caccgtctcg aaaccggccg attttctgcc cggagtggtt cgcggaatgg
     2161 tgttcgcggc actgtggttc atacacccat attcggcaat cgtctggggc ttgctggtcg
     2221 ctgtcgcggt ggtcgttccg ccgatcggca ccagcctcaa aagatcgctg ttcatcaact
     2281 tgccggcgct cgccggaccg gcactggtgg ccgtcttcat cacctggtcc cagcaggatc
     2341 cggtggtcgc ggcgtcaaac gcacagaccg gtctgtggaa gctgggctat cccccgataa
     2401 tctatccgat agtctatgga ccgtggatgc tgcttccgct ggggttggtg atgcgccgca
     2461 acacgattga tcgccacgtc atgcgatggc tgctgctgtg gttcgtcacc ggtttcgcga
     2521 tgatggccaa cccgtttgtg accggggtga aatttcagtt ttcgttcctg gttccattcc
     2581 tgatcctgca gattgccgga ctgttcgccc tggttcaaag gcggcaggcg gcgggcgggc
     2641 ggacttcatg gccgggcggc gccattgccg ccttcgtggt tctggcgtcg ctgaattcgg
     2701 ccgcggggct ggtgctggac gtcaacccga cggcgacaag gacggcggcg cgggccggaa
     2761 aggatcacct tgcactcctg aaggaacttg aaacgcttcc ggacggtggg gtcctgtgcg
     2821 accctttcga tgcccagatt gttccgtgga aagccgggaa gcctgtgttt gtcggccagt
     2881 ggttcctttc gacgcggtat caggaaaagg ccgcgcttgt gcgctggttt tattccgatg
     2941 aaccggatga ggcgcggacg gccggcttcc tgaacaacgc cggaatcagg tggattcttt
     3001 acgggccacg cgagatgcaa cttggaaaaa tgccgcgcgc cgacggtctg cggcttgtcc
     3061 gccagattgc cgggcgccag atctgggaat gggcgcctgc cggcgctgaa tggccgtcaa
     3121 tccttcagca gtccaaatga aaagcgtact ccggccagac cggtatttcc ggaaacgtac
     3181 ctgaagcgat cggaaccgga gtcgggaacg aacagatccc gggagtagag ggcgaacaca
     3241 ccgatgtact tccagacggt gacgtcggcg gccacggaac agaagatcct gtccgccagg
     3301 cgctggcggc cgtcgacccc gaatcccctt gcatagacgg ttccggtcag aaggagcctg
     3361 tccggcacct tgaccgtcag gcccgcgcga atcctggaaa atgccccgta ctgatcgacg
     3421 tcggcaccga tccaggccgt cagccaggga tcccagccgg ccatcattcc aaatgcgccc
     3481 ccatgcctcg cgggacgtga tgccccggtg actaccctct tggacaccgg accttccgcc
     3541 gcgtccctga aggcattgaa gtcgtatcga tcggccatgt agacattgtc gaaataaccg
     3601 gtcacgaacc cggattccat gaagtcgtac cggcctgtca cggtaaggac gaccttcgac
     3661 ctgatgttga acgcgagttc aaggccgacg tgggatgcat ccgaccatcc gatccttgac
     3721 catgacgccc agggctttat ctggatggcc cgcgccggcc gaaccggcca ggaaacatcg
     3781 atcgaccagg cggtcaacgg cgaggggtcg gtcgcaacca ggccgttcgg accgggaacc
     3841 gatgacgtcg ggttttccgg ggtggacgct cccaggtttg aaggtgcgtt gatatcgccg
     3901 gcaaacgaag caccgatctc aagccgcccg atctgtcccg atcgatcgac gaaccacagc
     3961 ggcctgaaat agacccgacc gccgatcacg gacggcccgg cgacaccgtc aagaaacagg
     4021 tctccgccag ccacatccca gtgaagtctg gcgctgactc cggttctgaa atgatccccg
     4081 tcgatgactc cgttgaacat ggaaacaagg ttacccatgc ccaggttgaa attctggact
     4141 ggtttgatgg aaacatcgaa tcgaccggtt ctgtcggtcc agccgaatct ggacagaaga
     4201 cggccgaagt cgttcggctc atcccattcc cgcatccgaa agccgtccgg atcgaaacgt
     4261 atcggcgcga tcacggatac ctcgaaacca tccctgccgc cggaaagcgc gaagtcaaaa
     4321 ccggctcgcc atgattcgtg gctgccgccg gcgaccccgg aaaactggag ttcggcaaag
     4381 ggccgccccc attcggattc actcgaaccg gatttggccg acttgtcgga ccccggattc
     4441 tgggaagcat gcgcggcccc ggccaggaac atcaaaaaaa ccgcggcaat gctgtaataa
     4501 gggcgcatca tggcaaccta ttctgcacgc acatcgaaca tcatgacaaa gtgacgtacc
     4561 gcccaaccgc cttcggccgg caccggtgat ttgaacacga acgcatcgtg ctgctataat
     4621 gcgcgcgccg acggaggctg ctgggatgac ggtcagcaac agacagatgg ctttgatcct
     4681 gcttttcttc gcaatctgcg cgggggccgg atacgcggtc tattggtggc acgagacaag
     4741 cctggatcgc atcgccatgt tccacgtcag cttcaagggc gaatccctta atcgggtgtc
     4801 ggccgccctg gcggacaagc gccctgccca ggcgcgtgaa atccttcaaa ggcagcccct
     4861 gtttgaaaaa atcggaatac tgaaggttct ttccagggac gaagccgtcg cggtaaggat
     4921 gttcgccgtc cgctgcatga ggccgctgcg cgatgttccc cagatacgcg ccgaacttgc
     4981 caggcttgcg gccgaggatc ccgaggaatc agtgaccagg cttgcttcca gggcacttca
     5041 gggaaggcgc tgaccggctc accccgaact acttccgtat cgcaag
//
LOCUS       DAOPPA010000217         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_17955_length_5075_cov_3.350996, whole genome shotgun sequence.
ACCESSION   DAOPPA010000217 DAOPPA010000000
VERSION     DAOPPA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17955_length_5075_cov_3.350996"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKH54_07570"
     CDS             complement(<1..678)
                     /locus_tag="PKH54_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOC99788.1"
                     /translation="MSEKRIVVVVPTYNEAQNVRNIVPEILSALPTADVLVVDDSSPD
                     GTGQIVTEMSASEPRLKLLTRKGKEGLGKAYVAGFKHCLEQGYDIIFQMDCDFSHQPS
                     HLKDFMAKITDYDLVLGSRYIPGGATTDWGFHRKFLSRGGNLYARTILGVPVSDLTGG
                     FKCWRKEVLLAIDLDSIAAAGYAFQMEMTFRSIKKGFKVAEIPIIFPDDQGRIKAGRR
                     DFLGKPGH"
     gene            838..1794
                     /locus_tag="PKH54_07575"
     CDS             838..1794
                     /locus_tag="PKH54_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99789.1"
                     /translation="MKKTTILAAVFISLVIVPAAALSRTDSPSARALALGDAVTAIGM
                     GTDGLFVNPGAIGQVTQYSVNIGYGYANINQWHQASASFVDSQTNKWLTGGLAYTFGY
                     ADLAGDTMTHDIRVALGTKYATEKVFIGFGVGMRIMNLSSDRSKSTWYVDTDIGALLG
                     IMKVFSIGITGRNLVQDAAQPKKNLARVELDKPRQYWNLAPRKLAVGVGFSYSIFNMG
                     IDCDVDFTTDIDGRGGSDTTPSLLAGMEFMIKNVVSLRGGVAWDRVGHFVPDDDRFEP
                     EVRLSLGVGYVSKAVAVDVGYAHDVLDASGFYLQTTVRVFLP"
     gene            1899..2747
                     /locus_tag="PKH54_07580"
     CDS             1899..2747
                     /locus_tag="PKH54_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HOC99790.1"
                     /translation="MSRKAFASMFVVVIALFAVACGGGGVPQFDTGNDVEYDVSIPDT
                     IDDNANPDVGNDVEPDVRPEVEMDVESELPPVDAKEIIDDVPPDVVPDGGEDIPEEVD
                     VIDFCPCNTAVNEPVCGVNDVTFTSPQCAACSLCAPDPLNCPSCTGVIACQGKPENFI
                     KRMTACDVCPCNLQTECERLSREIDQCKEVCGVDGTTEYADLCALKTAKNCDNGYDEH
                     IGSFGKCVVACPACEEFDNDPKNALANVAVCGSDGNTYKNVCWLNNCPLVEGATKLCN
                     QACPCP"
     regulatory      2798..3001
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3020..4933
                     /locus_tag="PKH54_07585"
     CDS             3020..4933
                     /locus_tag="PKH54_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC99791.1"
                     /translation="MKSSRYPIAIIVTTILFFLPGNLFAAEVLPELVEVPEIDVSADP
                     VAAMVPVRDSSASDLVVDRSTLESARGPAAGVVCGPAVLVRESGAPGQAASVSIRGSD
                     PRSTLVTLDGIPLNSPFLGGADLNSMTLVTLDSISTTRGGQGSLRGYDSTGGTVDAGT
                     PSILDSNGFTRAAVLAGSFGTFRLKAAHAHVFNATVPVGLMASAGVAHSGGTFSFLDS
                     NGNLRERTHNASTAIQAMAKVESEPAPGHRLDTVVEFFRADRQIAGLEQFPSTTAGQV
                     DTRAIAGLSWRGPRMFGIRGSSIARVWFRSLLFEYADKSPQFGPPVDTALLSNGFGAS
                     AFTLSAPLERLSIRLGLDGGWVSGQVRRVGQSDYRPGRGNVAAVVGLQGGAPSDNWGV
                     GADVRLEYAGGAGFMAVPGLGVWYEPHRLVRLSANVARSCRLPTLEELYFDTGYVRGN
                     PSLRPEDSITWDVGIHVGREKWWQFKASYFENHGFNMIIFAPESAFVIRAANSGSATL
                     RGVETSARVEWKWLEFSANYTWLFSRLESTGARMPARPEHVFSGLAGFRKGPLRLFAA
                     PSWQSGFFLDTYQSVREEGRFRLDLGLEIRPRPFLVMAVDFLNVTDKKDSVDFVQQPL
                     PGFSAFGSIRIEL"
BASE COUNT          959 a         1418 c         1549 g         1149 t
ORIGIN      
        1 atgcccaggc tttcccagaa aatcccgccg accagctttg attcgccctt ggtcgtccgg
       61 aaagatgatc gggatttcgg cgaccttgaa acccttcttg attgatcgga acgtcatctc
      121 catctggaac gcgtatccgg cggccgcgat cgaatcgagg tcgattgcaa gcaggacttc
      181 cttgcgccag cacttgaacc cgccggtaag atccgacacc gggacgccca gtatcgttcg
      241 tgcgtaaagg tttcccccgc gtgacaggaa cttgcggtgg aatccccagt cggtcgtggc
      301 gccacccggg atgtatctgg agccgagcac caggtcgtaa tcggtgatct ttgccatgaa
      361 atccttcagg tgggacggct ggtgcgaaaa gtcgcaatcc atctgaaaaa tgatgtcgta
      421 tccctgctcc aggcagtgtt tgaacccggc gacgtacgcc ttcccaagcc cttccttgcc
      481 ctttcgtgtc agcagcttca ggcgtggttc ggatgccgac atctccgtga cgatctgtcc
      541 agtcccgtcc ggcgacgagt catccacgac cagcacgtcg gccgttggga gggccgaaag
      601 aatctcgggc acgatgttcc tgacgttctg ggcttcgttg tacgtgggaa cgacaaccac
      661 gatccgtttt tccgacatat aagacctccg aatggttcaa cagccggttc cgtccagcct
      721 atcacgcccc ggtctgaagg gccagccgtc gatttcttga cagtttcggc acgcatcaat
      781 accgttagtg cgtccgtgcg cgactttcgc ggacgccttc tgtctgtggc ggttcgaatg
      841 aagaagacga caatccttgc ggccgtgttc atttccctgg tcattgtgcc cgcagcggcc
      901 ttgagcagga ccgacagccc ttccgcccgg gcgcttgcgc tcggggacgc cgtgactgct
      961 atcggcatgg gaacggacgg gctgttcgtc aatccgggtg ccatcggcca ggtgacccag
     1021 tattcggtga acatcggcta tgggtacgcg aatatcaacc agtggcatca ggcatcggct
     1081 tcgttcgttg attcccagac gaacaagtgg cttaccggcg gtctggcata tacgttcggc
     1141 tatgcggatc tggctggcga caccatgacc cacgacatca gggtcgcgct cgggaccaag
     1201 tatgccacgg aaaaggtgtt catcggtttc ggggtcggga tgcggatcat gaacttaagt
     1261 tccgatcgca gcaaatcgac ctggtacgtt gatacggaca tcggcgccct tcttgggata
     1321 atgaaggttt tttccatcgg cataactgga agaaacctgg ttcaggacgc cgcacagccc
     1381 aagaagaacc ttgcacgggt tgaattggac aagcctcgcc agtactggaa tcttgccccc
     1441 aggaagctgg cggtcggcgt gggtttttca tactcgatat tcaacatggg catcgactgc
     1501 gatgtcgatt tcaccaccga tatcgacggc aggggcggct cagatacgac gccaagcctg
     1561 ctggccggaa tggagttcat gatcaagaac gtcgtatcgc ttcgtggcgg cgtcgcctgg
     1621 gatcgcgtgg gtcattttgt tcccgatgat gatcgtttcg agcccgaagt caggctttct
     1681 cttggcgtcg gatacgtttc caaggccgtc gcggttgacg tgggatacgc tcacgacgtc
     1741 ctggatgcat ccggttttta tcttcagaca accgttcgag tgttcttgcc ctgaaactgt
     1801 tattacaata aactgttgcg tggattatcc agataggttc ggtccggaca gtcccggggg
     1861 tgttctcggt tatctttcaa gcgtttccgg aggattgaat gtccagaaaa gcttttgctt
     1921 ccatgtttgt ggttgtgatt gccctttttg ccgtcgcgtg cggcggtggt ggagtgcccc
     1981 agttcgatac gggcaacgac gtcgaatacg atgtttccat cccggatacc attgatgata
     2041 acgccaaccc tgacgtcggg aatgatgttg aaccggacgt caggccggaa gtcgagatgg
     2101 acgttgaatc ggaactgccg cccgtcgatg caaaggaaat cattgatgat gtaccgcccg
     2161 acgtggtgcc cgacggcggg gaagacatcc cggaagaggt tgatgtcatt gatttctgtc
     2221 cgtgcaacac ggccgttaat gaaccggttt gcggcgttaa cgacgtgacg ttcacaagtc
     2281 cccagtgcgc agcatgttcc ctttgcgcgc cggatccttt gaactgcccg tcgtgcaccg
     2341 gcgtgatcgc atgccagggc aagcctgaaa atttcatcaa gcgcatgacc gcctgcgacg
     2401 tctgcccgtg caaccttcag accgaatgtg aaaggttgtc cagggaaatc gaccagtgca
     2461 aggaagtctg cggtgttgac ggcacgaccg agtatgcgga cctgtgcgcc ctgaagacgg
     2521 ccaagaactg cgataacggc tatgacgagc acatcggttc tttcggcaag tgcgtggtcg
     2581 cctgtcctgc gtgcgaggaa tttgacaacg acccgaaaaa tgctcttgcg aacgtggccg
     2641 tatgcggctc ggacgggaac acatacaaga atgtgtgctg gctcaacaac tgtccgctcg
     2701 tcgagggcgc gaccaagctc tgcaatcagg cctgcccgtg cccgtaatcg tcttgccctt
     2761 gccccaatcc ttgacgaaac cagcgtaacg tgacaccttc aggccgttct ttccgggtgg
     2821 acgcccagcg tcccggagag ggaagcgggt gaaaatcccg cgcggtcccg ccactgtaaa
     2881 gggggaggat tttccggggc ccttccattc gccactggcc tgttgccggg aaggcggaag
     2941 ggcttcaggc gccagccgaa ccccccaagc caggagacct gcccggaagg ccgttgtccg
     3001 aactcctcga ggacagggag tgaagtcgtc acgttacccg attgcaatta ttgtcaccac
     3061 gattctgttt ttcctgccgg gcaatctgtt tgctgcggaa gtccttcccg aactggtcga
     3121 agtgcccgaa atcgatgtca gcgccgaccc tgtggcagcc atggttcctg tccgtgacag
     3181 ctcggcttcc gatttagtcg ttgacagatc gacccttgaa tcggccaggg gcccggccgc
     3241 cggcgtggta tgtgggccgg cggttcttgt aagggaaagt ggagcccccg gccaggcggc
     3301 ctcggtaagc atccgcggtt cggatcccag atcaacgctg gttacgctcg atggaatccc
     3361 gctgaattcg ccatttcttg gcggggccga cctgaactcc atgacgctgg tgacgctgga
     3421 ttcaatttca actactcgcg gcggccaggg atccctgcgc ggatacgact cgaccggcgg
     3481 aacagtcgat gccgggactc cttcgatcct tgattcgaac ggattcacca gggccgccgt
     3541 gctggccggt tcctttggaa ccttccggct gaaggccgcc cacgcccatg tattcaatgc
     3601 gacggttccg gtcggattga tggcctcggc cggggtcgct cattccggcg ggacgttttc
     3661 gtttcttgac tcgaatggga atctgcgtga aaggacgcac aacgcgtcaa ccgcaatcca
     3721 ggccatggca aaggttgagt ccgagccggc cccggggcac cgcctggata cggtggtcga
     3781 gtttttcagg gccgatcgac agatcgccgg tcttgagcag ttcccttcga cgaccgccgg
     3841 gcaggttgac accagggcca ttgcaggtct ttcgtggcgc ggtccgcgaa tgttcgggat
     3901 tcgtgggtcg tcaatcgcca gagtatggtt tcgaagcctg ctgtttgaat atgcggacaa
     3961 gtcgccgcag ttcgggcctc cggttgacac cgcactgctt tcgaacggat tcggggcgtc
     4021 ggcgtttacc ctttcagcgc ctcttgaaag gctttcgata aggctcgggc ttgatggggg
     4081 ctgggtttcc ggacaggtga ggcgggtcgg ccagtccgat tatcgtcccg ggcgtgggaa
     4141 cgtggccgcc gtcgtggggt tgcagggcgg ggcgccttcc gacaactggg gcgtcggtgc
     4201 cgatgtcagg cttgaatacg ccggcggggc gggattcatg gccgttcccg gacttggggt
     4261 ctggtacgag cctcatcgcc tggtaaggtt gtcggccaat gttgccaggt cgtgccgcct
     4321 gccaaccctg gaagagttgt atttcgatac cggatacgta cgtggcaacc cgtcgcttcg
     4381 gcctgaagat tccataacgt gggacgtcgg gattcatgtc ggtcgcgaaa aatggtggca
     4441 attcaaggct tcatacttcg aaaaccacgg cttcaacatg atcattttcg cccccgagtc
     4501 cgctttcgtc atacgggcgg caaattcggg atccgcgacg ctccgtgggg tcgaaacatc
     4561 ggccagggtt gaatggaagt ggcttgagtt ttcggcgaac tatacatggc tcttttcaag
     4621 gctcgaatcg accggtgcga ggatgccggc ccgtccggaa cacgtcttct cgggattggc
     4681 cgggttcagg aaggggcctt tgcggctgtt cgcggcgccg tcctggcaat caggattctt
     4741 cctggataca tatcagtcgg tcagggaaga ggggcgcttc cggctggatt tggggctgga
     4801 gatacggcca cggcccttcc tggtcatggc ggttgatttc ctgaacgtaa ccgacaagaa
     4861 ggattcggtc gatttcgtgc agcagccgct gcccggcttt tcggccttcg gatcgataag
     4921 gattgaatta tgagtaccgg ttgtttcaaa cccgctgtcg cagcctgttt gctgctggca
     4981 ttggttatgt ccgcgtgcat tgatggtcct ggtggattgg cggattcggc atcatggccg
     5041 gacgattcgg ggcagtcgct tccggaaggc aacat
//
LOCUS       DAOPPA010000218         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18027_length_5060_cov_3.715485, whole genome shotgun sequence.
ACCESSION   DAOPPA010000218 DAOPPA010000000
VERSION     DAOPPA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18027_length_5060_cov_3.715485"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKH54_07590"
     CDS             <1..578
                     /locus_tag="PKH54_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99792.1"
                     /translation="EGLVDALPREAIPGQHGLIVIFRDDAGRTSELDGLSSFLEAIEG
                     VDIVAGPPVGSAALRSLAGAVGFVRVTMAVMAAVLLFGTLFFVVGTLTRTMESRREEM
                     AILRLFGATGAYLKAPLYIQACVQGVAGLSGGVLMGLAIISSTNAYLLSELSVGTALP
                     SWPAMSLAAAIPGGIAVGLAGAIIASSRRLP"
     gene            575..1714
                     /locus_tag="PKH54_07595"
     CDS             575..1714
                     /locus_tag="PKH54_07595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC99793.1"
                     /translation="MKARRFRRATPGLVLSVMAMLATVPAAAGATDETTSTAETLKQE
                     LSILDALDSYVFEARQAESTAARTAGYLQAAMARLEKATSELAVATGEFEASRKRLAD
                     TIRIGRLTADYGVFESVFIPDRNLESTRRKMLVRKMAARQADDLAIMTAAHQKAVAME
                     FVAGMERAQAWVLARVSQDALAKLQADTEARRSLLNRIESDIKMYRRRAAELSDAEMS
                     MVRIIQARLSDRNVPVNFDAHKGSMPLPIADGVVKVPFGDVVHPRFKTVTPHPGWTIT
                     HDSRGPRNVRNIAFGRVVWTGRMRGFGTTVVVDHASGWYSVYAGLSTLKVSDGEVVRQ
                     GQVLAQVQAAPGDLEVTMYFELRKGSVAIDPADYVSQPDPPGGKP"
     gene            1711..2607
                     /locus_tag="PKH54_07600"
     CDS             1711..2607
                     /locus_tag="PKH54_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOC99794.1"
                     /translation="MNSGHLRRVGPGAILICLIALPALCGCASGPPRNLRGEDTGTSM
                     PTSPPEPERCPARLVPPGPPVPAGSAVVAIVIDDCGESPEQVIPFLGIPVPLSFGVLP
                     WHSESMNTSLLISARGFETLAHMPMEPHDPRWLENDWFLTTGMDREQIRIRIDRALDG
                     VPCATGMNNHMGSRFTADAPGMDHVMSRLKERGIYFLDSRTSDQSAAIAACSRQDARC
                     LSRDVFIDNEDDVEAITAQIKRLVEAARKNGCAIGIGHCRSKTAAAIMHYVRARDPGV
                     VFVPAGRIFEHCPSGQPQNLPE"
     gene            complement(2504..3436)
                     /gene="jag"
                     /locus_tag="PKH54_07605"
     CDS             complement(2504..3436)
                     /gene="jag"
                     /locus_tag="PKH54_07605"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOC99795.1"
                     /translation="MKEAIFTGRSSEEALLKASQELGVNISDMTYEVLDEETSLFGLF
                     RKDVRVVVRVREDAAQVVTRANYVPGYGDTGLVTTAGEILTLKKPSREKNVEALPGEL
                     PASGVVDSVEAGEFTAQPVRELRSQPETILSEKGPAASKVLGEILGLMGMECQVEFEE
                     NPESVSLNVSGGDYEMIVGRDGEVLAALQFIVNKIVNRFPDDRKLVILDAEGFRGRRE
                     QTLGKLARRLGDKAVATGRVIRLSPMTAQDRRLMHLALRENRKVSTRSEGVGDFRRLL
                     IIPAGFEAAQKDNVRKSDRPAQTPPRDRERGRNA"
     gene            complement(3484..>5060)
                     /locus_tag="PKH54_07610"
     CDS             complement(3484..>5060)
                     /locus_tag="PKH54_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOC99796.1"
                     /translation="APAAGAELAAPSVDASAEPAAVANEPQPAPVETWTTEPVDFWKE
                     YQQNPDYRVVQATDSRIVMVWPDPARFSSPLYIERTYSIGERYSLDATIRLISLSESE
                     IAGTMRLSVSAWESAAASGGYCGGMFGAPPDLLQAVCGTSKDVDLKNRKDILDNKGFE
                     ITESASYVGVSSRYFLSAVLPGSGNVAQCVASASEVGVVTALIRWGNESGGNQLIKAV
                     RGTPCIPDWLVGAHGMEGRLTCSQAASVLGVDPGIGIVELSEMKEDGLDPDKAAARQV
                     LMARRDRLVNFKFFSGPKDLGLLRSMSAGVENTIDFGWFGVLAKPMLWLMRVAHSVVP
                     SWGLAIIFLTIIVKLITLPFTHSSMKQMRRMSELKPRIDELQKKHKDDKVALNQATME
                     LYKREKINPLGGCLPMLIQMPIWIALYRTIYSAVDLYQAPLGLWIADLSSRDPYFVLP
                     VLLGVIMWVNQKITPVSGDPTQAKIMLWMMPIMFSAMMMFLPSGLVFYILINTVLSVF
                     HTLWNNRGLRRKAVAG"
BASE COUNT          994 a         1599 c         1504 g          963 t
ORIGIN      
        1 ccgaagggct ggtcgacgcc ctgccccgcg aggcgattcc cggccagcac gggctcatcg
       61 tcattttcag ggacgatgcg ggaaggacgt cggaactgga cgggctttcc tcgttcctgg
      121 aagccatcga aggcgtcgat atcgttgccg gacctccggt cggttcggcg gcccttcgat
      181 cgcttgccgg cgcggtcggt ttcgtaaggg tgaccatggc ggtcatggcc gccgtccttc
      241 ttttcggaac cctgttcttc gttgtcggca cattgaccag gacaatggaa agcaggcgcg
      301 aggagatggc cattctgaga cttttcggcg cgacgggcgc ctatctgaag gccccgcttt
      361 acatccaggc ctgcgtccag ggcgtggctg gactgtccgg cggcgtcctg atgggacttg
      421 caatcatctc gagcacgaac gcatacctgc tgtcggaact ttcggtcggc accgcgctgc
      481 cgtcgtggcc tgccatgtcg cttgccgcgg cgatcccggg cgggatcgcg gtcggacttg
      541 ccggtgccat catcgcatcc tccaggagac tcccgtgaag gccagacgtt tccgccgggc
      601 cacgccgggc ttggtactgt ccgtcatggc aatgctcgca acggttccgg cagccgccgg
      661 ggcgaccgat gagacgacct cgaccgcgga gactctgaag caggaactgt ccattcttga
      721 cgcgcttgac agttatgtgt tcgaggccag acaggcggaa agcacggccg cccgtaccgc
      781 agggtacctg caggcggcaa tggcgaggct cgaaaaggcc acctcggagc tggctgtcgc
      841 gaccggggag ttcgaggcat cccggaagcg gcttgccgac acgataagaa tcggcagact
      901 gaccgccgac tacggcgtat tcgaatctgt cttcattccg gatcgcaacc tggaatccac
      961 gcggcgcaaa atgctggtaa ggaagatggc cgcacgccag gccgacgacc tggcgataat
     1021 gaccgccgcg caccagaagg ccgttgcaat ggagttcgtg gccgggatgg aacgcgcgca
     1081 ggcatgggtg ctggccagag tcagccagga tgccctggcg aaacttcagg cggacaccga
     1141 ggcgcgccgc agcctgctga accggatcga aagcgacatc aagatgtacc gcaggcgcgc
     1201 ggccgaattg tcggacgccg aaatgtcgat ggtccggatc atccaggccc ggctttccga
     1261 ccggaacgtg ccggtcaact tcgacgcaca caagggaagc atgccgcttc cgattgccga
     1321 cggagtcgtc aaggttcctt tcggcgacgt cgtgcatccc aggttcaaga ctgtgacgcc
     1381 gcacccgggc tggacaatca cccacgactc caggggcccg cggaacgtgc gcaacatcgc
     1441 tttcgggcgg gtcgtctgga ccggcaggat gcgcgggttc ggaacaaccg ttgtggtcga
     1501 tcacgcctcc ggctggtaca gcgtctatgc cgggctttcc actctcaagg tttccgacgg
     1561 cgaggtcgtc cgccagggcc aggttcttgc ccaggttcag gcggctcccg gcgatctgga
     1621 agtcacgatg tacttcgaac tgcgcaaggg ttcggttgcg attgacccgg ccgactatgt
     1681 ctcgcagcct gatccgcccg ggggaaagcc atgaactccg gccacctgcg ccgcgtcggc
     1741 cccggcgcga tccttatatg cttgattgcc ttgccggcct tatgtggctg cgcgtccggc
     1801 ccgccgcgca acctgcgtgg cgaagatacc gggacttcga tgcctaccag tcctcccgaa
     1861 ccggaaaggt gccccgccag actggttccg ccaggacccc cggttccggc gggctcggcc
     1921 gtggtggcaa tcgtgattga tgactgcggc gagtcgccgg aacaggtgat acccttcctc
     1981 gggattccgg ttccgctctc gttcggcgtt ctcccctggc attccgaatc aatgaatacg
     2041 tccctgctca tttcggctcg tggattcgag acgctggccc acatgcccat ggaaccgcac
     2101 gacccgcggt ggctggaaaa cgactggttc ctgacaaccg ggatggatcg cgagcagatc
     2161 cggatccgaa tcgacagggc tcttgatggg gttccgtgcg ccaccggcat gaacaaccac
     2221 atgggatcca ggtttacggc agacgctccc ggaatggatc acgtaatgtc aaggcttaag
     2281 gagcgcggca tatatttcct tgattcaagg acgtcggatc aatcggccgc gatcgccgcg
     2341 tgttcaagac aggacgcgcg ctgcctttcg agggacgtgt tcatagacaa tgaagacgat
     2401 gtggaggcaa tcaccgcaca aataaaaagg cttgtcgaag cggccaggaa gaacgggtgc
     2461 gcaatcggca tcggtcattg cagatcaaaa actgccgccg ccatcatgca ttacgtccgc
     2521 gctcgcgatc ccggggtggt gtttgtgccg gccggtcgga ttttcgaaca ttgtccttct
     2581 gggcagcctc aaaacctgcc ggaatgatca gcagccgcct gaagtccccc acaccctcgc
     2641 tcctggtgga aaccttgcgg ttttcccgca gggccaggtg catcagccgc ctgtcctggg
     2701 ccgtcatcgg actcagcctg atgacgcggc cggttgcgac ggccttgtcg ccaagccgcc
     2761 ttgcaagctt gccaagcgtc tgctcgcgac gccccctgaa accctcggcg tcaagaatta
     2821 ccagcttacg gtcatcgggg aacctgttga cgatcttgtt aacgatgaat tgaagggcgg
     2881 ccaggacctc gccgtcgcgc cccacaatca tctcatagtc gccaccactg acattaaggc
     2941 tgacggactc gggattctcc tcgaactcga cctggcactc cattcccata aggcccagga
     3001 tttcccccag aaccttcgac gccgccgggc ccttttccga aagaatcgtt tcaggctgcg
     3061 atcggagttc gcgaaccggc tgggcggtaa attcgccagc ctcgaccgaa tcgaccaccc
     3121 cggacgccgg cagttcgccc ggcaacgcct cgacgttctt ttcacgggac ggcttcttca
     3181 gcgtcagtat ctcgccggca gtcgtcacca gcccggtgtc gccgtaaccg ggaacgtagt
     3241 tggcgcgcgt cacgacctgg gccgcgtcct ccctgacccg aaccacgact ctcacatcct
     3301 ttcggaaaag tccgaaaagc gaagtctcct cgtcaagaac ctcgtacgtc atgtcactga
     3361 tgttgacgcc aagctcctgg cttgccttca gcagtgcttc ctcacttgat ctgccggtaa
     3421 atatcgcttc cttcatcacg aatcctccgc cccgaaatgg catggggcca gcgcgatcgt
     3481 ttttcagccc gcaactgcct tcctacgaag cccgcggttg ttccagagcg tgtggaagac
     3541 cgaaagcacc gtgttgataa gaatgtagaa gacaagcccg gacggcagga acatcatcat
     3601 cgccgagaac atgatcggca tcatccacag cattatcttc gcctgggtcg ggtcgccgct
     3661 cacgggcgtt atcttctggt tcacccacat gatcacgcca agaagcaccg ggagcacgaa
     3721 atagggatcc ctggacgaaa ggtccgcgat ccacagcccg agcggggcct gataaaggtc
     3781 aaccgcgctg tagatggttc ggtagagcgc aatccagatc ggcatctgga taagcatcgg
     3841 aagacatccg ccaagcggat tgatcttctc gcgcttgtaa agctccatcg tggcctggtt
     3901 cagcgcgacc ttgtcgtcct tgtgcttctt ctgcagttca tcgatcctgg gcttcagttc
     3961 ggacattcga cgcatctgct tcatgctcga atgcgtgaac ggcagcgtga tcagcttgac
     4021 aatgatggta aggaaaatta tcgccagacc ccacgacgga accaccgaat gggcaactcg
     4081 catcagccac agcatcggct tggccagaac gccgaaccag ccgaaatcaa tcgtattttc
     4141 cacaccagcc gacatcgagc gcagcagtcc gagatccttg ggcccggaaa agaacttgaa
     4201 gttgacgagg cggtcgcgcc tggccatcag cacctgtctt gctgcggcct tgtcgggatc
     4261 gagcccatcc tccttcattt ccgaaagctc gacgatgccg atcccgggat cgacaccaag
     4321 caccgaagca gcctgggaac aggtgagccg tccttccatg ccgtgcgcgc cgacgagcca
     4381 gtcggggatg cagggagtcc cccgaaccgc cttgattagc tggtttccac cagactcgtt
     4441 tccccatcga atcaaagccg tgacgacgcc gacctcggac gcggatgcaa cgcactgggc
     4501 aacgtttccg gaacccggga gcactgccga aagaaagtac cttgaagaca ccccgacata
     4561 cgacgcggat tcggtgatct cgaatccctt gttgtccagg atgtccttcc ggttcttcag
     4621 atcgacatcc ttcgacgttc cgcagaccgc ctgcagaaga tctggcgggg cgccgaacat
     4681 gccgccgcag tatcccccgg aagcagccgc cgattcccat gcggacacgg aaagacgcat
     4741 cgtgccggcg atctcgcttt cagaaaggct gatcagccgg atcgtggcat caagcgagta
     4801 acgctcgccg atggaatacg tgcgttcgat gtaaagggga ctcgaaaacc gggccggatc
     4861 cggccagacc atcacaatcc gcgagtcggt cgcctgcacc acgcgataat caggattctg
     4921 ctgatattcc ttccagaaat cgacgggttc cgtggtccac gtctccactg gggctggctg
     4981 cggttcgttc gcgacggcgg caggctcggc ggacgcgtcc acggacgggg cagccagttc
     5041 cgcaccggcg gctggcgcgg
//
LOCUS       DAOPPA010000219         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18072_length_5051_cov_3.761009, whole genome shotgun sequence.
ACCESSION   DAOPPA010000219 DAOPPA010000000
VERSION     DAOPPA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18072_length_5051_cov_3.761009"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKH54_07615"
     CDS             complement(<1..853)
                     /locus_tag="PKH54_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99797.1"
                     /translation="MRFASSLIMLVAIVLAGCSGRTPPPNPAAAGGEAAPVQSTAVEQ
                     VAKQPPAPPPSRHAALREGIDGWLKLASTCQKRWFAKVDPAKLDKWELPVDVPSMEDD
                     CDKLVFDFEALSKENLLKGPELDSLFGRMAMVTDLYTHLALRCRKVGVKDRVPFKQMI
                     AGLRDRLGAEVEAVANEGAAVLALDDDMLVRDVSGQAMMDRALGALGRVKSDMDEWIV
                     KPRKEVGPVFKYSLKVSGRLAAGASEALAADASLPEKFRMSASALSKAWTVVSDFYAG
                     DYLAEEEA"
     gene            complement(874..2415)
                     /locus_tag="PKH54_07620"
     CDS             complement(874..2415)
                     /locus_tag="PKH54_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99798.1"
                     /translation="MGRFLVLLAVAGAVAVGGCVNKEEMAARQKEAFEARKKEVQAEL
                     AVVLQGWLDEMTRTLPADIKKYPKVQSPLVKWRADTFNFDWRRQVDAAVVKARFLAVA
                     SEFEAIPKFFDVMEKFWKKEVMFKDYMVAWKELKRTSSDPLAGLLADFDHTFVHVEAF
                     YGAQDMEGDDRAIYFFRHWQVAFEFPREEKESVGEYIARLCEAKLKDYCMSVPFEDLH
                     FALEKPYLTEARRIVKAYLEEHPGVELNRIFEQYLADVDARLAAWKDFPETLVVPTMK
                     SRNPYTSQLLFTVDGQSVDYEGRDYLKFGKDWAVSESEWKAFAKALVPEVEKLSEERG
                     PENMEVALLKMDRAAPMAVSARIVEAIRGTTARYVQFAGRNRSDAVAKLTKVGNLQFR
                     EVPELPAKLDVAGVGKLACRGLGQSIDDQAFPSKVGPVVYLGTSGIKTGVMKDGSVVD
                     LVDADQAAAVKALLSAESMLLVDEKVSYEAFTTLIEPVFINCDNEVCKYPRNNEPRVG
                     VSICR"
     gene            complement(2548..3372)
                     /locus_tag="PKH54_07625"
     CDS             complement(2548..3372)
                     /locus_tag="PKH54_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOC99799.1"
                     /translation="MTSLLVALTIASTVMTIPDNPCPSGWKYMLAEVRAGERDPTDIA
                     RRLMVKPDMLADWNRMSVRERLQQGQLLVFCKDLRPASVGSPSRGRLRAGVNMDADGD
                     SRGCGWVKSPLRTTTWGTPETVAAVSDCLCRFKTRFPGAPDVTLGDLSRQGGGRLGRH
                     LSHRSGRDVDLGYITNPPQSGGYFNRNARYGNLDAEKQWWIVKCFLDRGNVRFIFLNW
                     PALSALKSFVKSRPELSFYMRFFPGGSSPVISADREHRNHLHVRFDCPPDDIECIE"
     gene            complement(3483..4286)
                     /locus_tag="PKH54_07630"
     CDS             complement(3483..4286)
                     /locus_tag="PKH54_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99800.1"
                     /translation="MPRAIITQMVLAWLLGIVIAVPLEAAEPESSWPADGSFVRITRE
                     GGVTPYISVYYDVTVRGRTLVVTMTKDTLCRVMTHERVVILDGSDARRVLGILSDAGA
                     WIMRPPVRDRGTVETAPGSPDDDAAYEFWSASGRDMRRFRATRRELLGDPGLTRLLSV
                     VRATVTGLVEPLALRDVFQPADRTGILAITASEPAVALIDGWDRVRLPVSSLDLKVGV
                     HEVVVTGKSGSRRNFTVRVVPGENPAIHVLLGDPPPPGVTAEPSVSPVP"
     gene            4372..4998
                     /locus_tag="PKH54_07635"
     CDS             4372..4998
                     /locus_tag="PKH54_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99801.1"
                     /translation="MECRAIARPSRHDLEPWLGGSAYSTAARLFLRNADRRATEAFRL
                     AWNKVRQELAASFGTKRCDYDSVNAVLDRRVFKAPPDAVPALEEFGMPLPIALAASSS
                     ACMVGDIELALSWLASAADSGEGPALAAYIVLLSASSPDSALALVTPTDGMPAEVAAA
                     GCLASMRVGIRNENLCAATNRHADNRQAAAIQEIIEMEADQAVGKTVQ"
BASE COUNT         1048 a         1635 c         1396 g          972 t
ORIGIN      
        1 tcgcttcctc ttctgcgagg tagtcgccgg cgtaaaaatc ggacaccacg gtccaggcct
       61 tcgaaagcgc cgacgcagac atcctgaact tctcgggaag cgatgcatcc gccgccagtg
      121 cttcggacgc gccggccgca agcctgcctg aaaccttcaa tgaatacttg aacaccggcc
      181 cgacttcctt ccttggcttg actatccatt cgtccatgtc ggacttcacg cggccgaggg
      241 cgccaagggc acgatccatc atcgcctgcc cgcttacgtc gcgaaccagc atgtcatcat
      301 caagagccag caccgccgcc ccttcattcg cgaccgcctc gacctcggcg cccaggcgat
      361 ccctgagtcc cgctatcatc tgcttgaacg gaacccggtc cttgacgccg accttgcggc
      421 agcgaagcgc caggtgggta tagagatcgg tgaccattgc catccggccg aacagcgaat
      481 ccagttcagg tcccttcagg agattttcct tcgaaagggc ctcgaaatca aaaaccagct
      541 tgtcgcagtc gtcctccatg gacggcacgt caaccggaag ttcccactta tccagcttcg
      601 ccgggtcaac cttcgcaaac caccgcttct ggcaggtcga agccagtttc aaccaaccgt
      661 cgatgccttc gcgaagggcg gcatgcctgg aaggaggagg cgcgggcggc tgcttggcca
      721 cctgttcaac ggcggtggac tggactggag ccgcctcgcc gccggccgca gccggattcg
      781 gtggcggggt tcttcccgaa cagccggcca gcactatcgc aacaagcata ataagagacg
      841 atgcgaatct catgaagttg aacctccgtc gaatcacctg caaatcgata ccccaaccct
      901 gggttcgtta ttgcgcgggt acttgcagac ctcgttgtcg cagttgatga ataccggttc
      961 aatcagggtg gtgaaggcct cgtacgaaac cttttcatcc accagcagca tggactcggc
     1021 ggaaagaagc gccttgacgg cggctgcctg atccgcatcc acgagatcga ccacggatcc
     1081 gtccttcatg acgcctgtct tgattccgga agtccccaga tacacgacgg gacccacctt
     1141 cgacgggaag gcctgatcat cgatcgactg gcccagccca cggcatgcga gcttgccgac
     1201 gccagcgacg tccagcttcg ccggcagttc gggaacttcc ctgaactgca ggttgccgac
     1261 cttcgtcagc ttggccaccg catcgctgcg attgcggccc gcgaactgaa cgtacctggc
     1321 ggtcgttccc ctgatagcct cgacaatcct ggcggaaacg gccatcgggg cggccctgtc
     1381 catcttcagc agcgcgacct ccatgttctc cgggcccctc tcctctgaaa gcttctcgac
     1441 ctctggcaca agagccttgg caaaagcctt ccattcggac tccgaaacgg cccagtcctt
     1501 tccgaacttc aaataatccc tgccctcgta atcgacggac tggccatcga ccgtaaatag
     1561 aagctgtgat gtgtaggggt tacgcgactt catcgtcgga accacaaggg tttccgggaa
     1621 atccttccat gcggcgagtc ttgcatcgac atctgcaagg tactgttcaa aaatccggtt
     1681 gagctcgacg ccggggtgtt cctcaaggta cgccttcaca atacggcgcg cttccgtcag
     1741 gtacggcttc tcgagcgcga aatgcagatc ctcgaatggc accgacatgc agtagtcctt
     1801 caacttcgcc tcgcaaagcc gtgcgatgta ctccccgacg gattcctttt cttcccgcgg
     1861 gaactcgaac gcgacctgcc agtgccggaa gaagtagatc gcccggtcgt caccttccat
     1921 gtcctgagcg ccgtagaacg cctcgacatg aacgaatgtg tggtcgaagt cggccagcaa
     1981 tccggccagc gggtcgcttg acgtacgctt cagttccttc caggcgacca tgtagtcctt
     2041 gaacatgacc tccttcttcc agaacttctc catcacgtcg aaaaacttcg ggatggcctc
     2101 gaattcagat gcaaccgcaa ggaaccgggc cttgaccacg gcggcatcaa cctgtcttct
     2161 ccagtcgaag ttgaaagtat cggcgcgcca cttgaccagt gggctctgaa ccttcggata
     2221 cttttttata tcggccggga gtgtcctggt catctcatca agccacccct gaaggacgac
     2281 ggccagttcc gcctgaactt ccttctttct ggcctcgaag gcctccttct gtctggccgc
     2341 catctcttcc ttgttgacgc atccgcccac agcaactgcg ccggcgaccg ccagcagcac
     2401 caggaacctt cccatttgaa cccccggaaa taccattgag ttgtccagac gcgcccgaat
     2461 cgatcgagcc taacccggcc gagacgcgtt gaaagcggat tatagattga aaaccacaat
     2521 gttacagatg aaaaatggcc gcccgcatca ttcgatgcat tcgatgtcgt cgggcgggca
     2581 gtcaaacctt acgtgaagat gatttcgatg ttctctgtcc gccgaaatca cgggcgacga
     2641 tccgccggga aaaaacctca tgtaaaaaga aagctccgga cgggacttaa caaacgactt
     2701 cagcgcggac agggccggcc agttcaggaa gatgaacctg acattgcccc gatccaggaa
     2761 acatttgacg atccaccact gcttttccgc gtccaggtta ccgtacctgg cgttgcggtt
     2821 gaaatagcct ccggactgcg gcggattagt gatatagcca agatcgacgt cccggccgct
     2881 gcgatgcgac aggtggcgcc cgagtcttcc accgccctgc cgcgaaagat cccccagcgt
     2941 cacgtccggc gctccgggaa aacgtgtttt gaaacgacaa agacaatccg aaacggcggc
     3001 aacggtctcg ggcgtgcccc acgtggtggt tcgcagcggt gacttgaccc agccgcagcc
     3061 acgactgtca ccgtccgcgt ccatattcac gccggcccgc aggcggccgc gcgacggact
     3121 gccgacggaa gctggccgca ggtccttgca gaacacaagc aactgtccct gttgaagcct
     3181 ttccctgacc gacatcctgt tccagtcggc cagcatgtcg ggcttcacca tcaggcggcg
     3241 ggcaatatcc gtgggatcgc gttcaccggc cctgacctcg gccagcatgt acttccaccc
     3301 ggaagggcag gggttgtccg ggatggtcat caccgtggag gcgatggtca gtgcgacaag
     3361 cagcgaagtc aatattcaag catccagggt tgacgtcaaa tcaacggctt caaccggttc
     3421 cgccaaagtg acggacggaa aacacatttt caggcaatct tcttccgggg tcaaaacttt
     3481 tgtcagggga cggggctgac cgaaggttcc gcggtaactc caggcggggg cggatcccca
     3541 agcaggacgt gtatcgcggg gttttcccca ggcacgacgc ggacggtaaa attcctcctg
     3601 gatccgctct ttccggtcac caccacttca tgcactccga ccttcaggtc aagcgagctg
     3661 accggaagcc tgacccgatc ccacccatca atcagcgcga cggcaggctc ggacgccgtt
     3721 attgccagaa tcccggtgcg atcggcgggc tggaagacat cccgcagggc cagcggctcc
     3781 acaagaccgg taacggtcgc gcgaaccacg gaaagaagcc ttgtcagccc ggggtcgccc
     3841 agaagttccc gcctggtcgc tcgaaacctt ctcatgtcgc ggccggaagc gctccagaat
     3901 tcatatgcgg catcgtcgtc aggcgatccc ggcgccgttt cgaccgtgcc ccggtcacgc
     3961 accggcggcc gcattatcca ggccccggca tcagaaagga tcccgaggac gcgccttgca
     4021 tccgaaccgt caaggatgac gacgcgctca tgcgtcatca cccggcacag cgtgtccttg
     4081 gtcatcgtca cgaccagggt gcgaccgcgc acggtcacgt cataatagac cgaaatatag
     4141 ggggtaaccc cgccttcgcg tgtaatccgg acgaagctcc catcggccgg ccaggacgat
     4201 tctggttccg ccgcctccaa cggaaccgcg atcacaatgc ccagcagcca cgccaggacc
     4261 atttgggtta taatcgctcg cggcatggga agaataccct taatcacatt gctgatcgtg
     4321 tttccggcca tcgggctcgg tgcaacgaat atagcaccat cgcagccccc agtggaatgc
     4381 cgtgccatcg cccggccttc tcgacacgat ctcgagccct ggctgggcgg ctctgcatat
     4441 tccacggctg cgaggctctt tcttcgcaac gccgacaggc gggcgacaga ggcttttcgc
     4501 ctggcgtgga acaaagtcag gcaggaactt gccgcctcgt tcggcacgaa acgctgcgac
     4561 tacgattccg tgaacgcggt tctcgatcgg cgcgttttca aggctccccc cgacgcggtt
     4621 cccgccctcg aggaatttgg catgccactg ccgattgcgc ttgcggcctc atcttcagcc
     4681 tgcatggtcg gtgacattga actggcgctg tcctggcttg cgtcggcggc cgactccgga
     4741 gaaggccccg cgctcgcggc gtatatcgtg cttctttctg caagctcgcc cgattccgca
     4801 ctggcgcttg taacgccgac cgacggcatg ccggccgaag tggcggcggc cggatgtctt
     4861 gcatcgatga gggtcgggat ccgtaatgag aacctttgcg cggcgacgaa cagacacgcg
     4921 gacaaccgcc aggcagccgc catccaggaa atcatcgaaa tggaggccga ccaggcggtc
     4981 gggaaaactg tgcagtgaaa ccggtttatc caacgggcca ggaaagggat cgattcaagg
     5041 acgactgcct g
//
LOCUS       DAOPPA010000220         5041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18116_length_5041_cov_5.062575, whole genome shotgun sequence.
ACCESSION   DAOPPA010000220 DAOPPA010000000
VERSION     DAOPPA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18116_length_5041_cov_5.062575"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKH54_07640"
     CDS             <1..945
                     /locus_tag="PKH54_07640"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HOC99802.1"
                     /translation="VVVVAIGRAEVVVVVVEARIHYFDNTTKMAVAQNDTANGFNPNL
                     WSRFDWTEVDGVKYFCHTTFDAGSMEDAANAFAPDATDPLTGGCGGYPWTPLTTTAPI
                     MNEVIESPGATFDGTGDPANATNGVRGGGATTGGTDVYSIGISPESWLVLRVSEHAVQ
                     NGPGPDFVVFENAFNYGDDGVFMDQAVVQVSRDGLTWVTFPFDYTSADETTYSTAPDD
                     WSGFAGISPVRLNQEATPDADPFDAATAGGDRFDLDDLPEADPDAVAIKNHGFTYVRL
                     VAAAAILNPDTDAPFVHDPIANGPDIDGIAVRHVFENQ"
     gene            complement(967..1812)
                     /locus_tag="PKH54_07645"
     CDS             complement(967..1812)
                     /locus_tag="PKH54_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOC99803.1"
                     /translation="MKLMSGLMPVFAVIVGVFLVLGAGSAGGAAKAPSGQGDDRVPTQ
                     VYGGVPLSTREDSAYVFIDNESFDIGYSEYRQNPVWVGYYVGPSLVPGRFKRPRTFQT
                     DRRTVSKVGHRDYTRSGYDRGHMAPNHAIATRYGAGGQAETFRMSNIVPQKPDLNRQV
                     WRRIEEMEADRWAVDLDGVWVITGPVFDEDRQFLKKAAPRIDIPDAFYKVIVDEVVAD
                     ESGAPVPRMLAFMVPQDVDGGESVEEFLVSVDWIESKTGLDLMPGLPDEVEAVLESAV
                     PDALW"
     gene            1929..3365
                     /locus_tag="PKH54_07650"
     CDS             1929..3365
                     /locus_tag="PKH54_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99804.1"
                     /translation="MSRVLLIKPRYIGPEFQSVSHPLGLMHIAAALRPSGHDVRIHDC
                     ASSPFRHRRLAELINAWRPELVGISLIVSELDTAASVTSVVRNSLPGTPIVLGGPWPS
                     ANPDAAIERFGADFVVAGEGEIVFPALVEAIRDGHAPSDITRDIPGVFCRVDGITTGS
                     RAGILTEEQLETLGFPAWDLIDRRIYARIPSMAGTGCGDYMTVMTSRGCPFMCAYCHQ
                     TLGKRFRARSAESVLAELDELHGRFGYSEFEIVDDCFNLDRERMSAILEGIATRLPGA
                     RLHFPNGLRSDRLEPADIKLMRRAGTVSACFAIETATPELQKLIGKNLDIGKARAAIE
                     CAVSQGIFSTGFFMLGLPTETLDQATATIDFASNLPIHRAIFMLTTPFPGTPLADMTA
                     NILKERGISQAGENLNYFTRGVNVSAMTDDELNRVFRDSYRRFYLDPRRVARILAAHP
                     RKLSLPRYGLMTLAKVLPGVWRTGAGRT"
     gene            3450..4922
                     /locus_tag="PKH54_07655"
     CDS             3450..4922
                     /locus_tag="PKH54_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99805.1"
                     /translation="MAEVLLIKAWAPGVDDRAMVPPIGVMQLAGILRQAGHQVRLLHV
                     GPIAVSREKILNAVADKRPDVIGISAITTEFLVYREIAGLLNESFPGIPVMAGGPLAS
                     SNPPAALGVPGVSVIAVGEADVTILELVDALAAGRDPAGLPGTAVLRNNIVVRGPNRE
                     PLTPAELDALPLPAWDLLDLDEHFRRRGMASVGIRPYMQVMTSRGCPYQCAYCHGLMG
                     RRYRARSPESVVNELRALREYFDIHEFEIVDDCFNLDRPRMHAILQGLIDFKDPLLRL
                     QFPGGLRSDILEKEDILMLRRAGTNFISFAIETASPRLQKMVRKNLNIERALESISTA
                     ADAGIFCNGFFMLGFPTETAEECRATIDLACSTRLHEALFFKVNPFRGTEMFDMAVQM
                     SGLDPAAIPLEEMDYFNVRHNLSAMPDAEFERLWTGAYRRFYFNPSRIIRIILRHPRK
                     LQLLMYLPQVVRKTVKSLLGGHMRMPGASKAGRNSGDKEN"
     gene            complement(4942..5019)
                     /locus_tag="PKH54_07660"
     tRNA            complement(4942..5019)
                     /locus_tag="PKH54_07660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4979..4981),aa:Val,seq:cac)
BASE COUNT         1031 a         1587 c         1453 g          970 t
ORIGIN      
        1 gttgtcgtcg ttgcgattgg acgcgcggag gttgtcgtcg ttgttgtcga agctaggatc
       61 cactatttcg acaacaccac gaaaatggcg gttgcgcaga acgacaccgc gaacggcttc
      121 aatccgaatc tctggagccg attcgactgg accgaagtgg acggcgtcaa gtacttctgc
      181 cacacgactt tcgacgccgg atcaatggaa gacgccgcga acgcgttcgc acccgacgcg
      241 accgatccat tgacaggcgg atgcggtggt tacccctgga cgcccctgac gaccaccgcg
      301 ccgatcatga acgaagtgat cgaatccccc ggggcaacct tcgacggcac cggagacccg
      361 gccaacgcca cgaacggtgt acggggcggc ggcgccacca caggtggaac cgacgtctac
      421 tcgatcggaa tctcaccgga aagctggctg gtgctgcgcg tatccgaaca tgccgtgcag
      481 aacggcccgg ggccagactt cgtggtgttt gaaaacgcat tcaattacgg cgacgacggg
      541 gttttcatgg accaggcggt ggtccaggtg tcacgcgacg gtctgacctg ggtaacgttc
      601 cccttcgact acacctcggc cgacgaaacc acatattcca cggcccccga cgactggtcc
      661 ggattcgcgg gcatttcacc cgtccgcctg aaccaggaag cgacaccgga cgccgatccg
      721 tttgacgccg ccacggccgg cggggacaga tttgaccttg acgacctgcc ggaagccgac
      781 ccggatgccg tggccatcaa gaatcacggg ttcacttatg tccgactggt ggcggcggca
      841 gcgatcctga atccggacac cgacgcgccc ttcgttcacg acccgatcgc gaacggtccc
      901 gacatcgacg gaatcgcagt caggcatgtg tttgaaaacc agtgatcccg cgtgaggcct
      961 gttgattcac cacaaggcgt ccggcactgc cgattcgagg acggcttcga cttcatcagg
     1021 aagacccggc atcagatcca ggcctgtttt tgactcgatc cagtcaacgc tcaccagaaa
     1081 ttcttcgacg gactcgccac cgtcgacatc ctgcgggacc atgaaagcca gcatcctcgg
     1141 aaccggcgcc ccggactcat ccgccacaac ctcatcgaca atgaccttgt aaaacgcatc
     1201 cggaatgtcg attcgcggcg cggccttctt caggaactgc ctgtcctcgt caaagaccgg
     1261 cccggttatc acccacactc cgtcaagatc gacggcccag cgatcggcct ccatttcctc
     1321 gatgcgtcgc cagacctgcc ggttcagatc cggtttctgc gggacgatgt tgctcatccg
     1381 gaaggtttct gcctggccgc cggcgccgta tcgcgtcgcg atcgcgtggt tgggggccat
     1441 gtgcccgcga tcgtacccgc ttcgcgtgta gtcacgatga ccgaccttcg acacggtccg
     1501 acggtcggtc tggaacgtcc gcggtcgctt gaacctgccc ggaaccagcg acggtcccac
     1561 gtagtatccg acccacaccg gattctggcg gtattccgaa tacccgatgt caaaggattc
     1621 gttgtcgata aagacataag ccgagtcttc cctggtcgac agcggcacgc cgccgtagac
     1681 ctgggtcggt actctgtcgt caccctggcc cgacggcgcc ttggccgcac ccccggcgga
     1741 accggcgccc agaacaagga agaccccgac aatgacagcg aagacaggca taagaccgga
     1801 cataagcttc atttccagat cgtcagtcga ccagattgaa ttctacaccg ggacgatcct
     1861 gttttacctg tccaggacag catccgtccc gatatctgtt aacattcccg ccggaggtcg
     1921 aacggtcaat gtccagagtc ctgctgatca agcccaggta catcggcccg gaattccagt
     1981 ccgtgtcgca tccgctggga ctgatgcaca tcgcggccgc gctccggcct tccggtcacg
     2041 atgtccggat tcacgactgc gcgtcaagcc ccttcaggca tcgaagactg gccgaattga
     2101 tcaatgcctg gagacccgag ctggtcggaa tcagcctgat cgtctctgaa ctggacaccg
     2161 ctgcatctgt aacgtcggtc gtcaggaatt cattaccagg caccccgatt gtccttggcg
     2221 gaccatggcc ctcggccaac ccggacgccg caattgagcg gttcggcgcc gacttcgttg
     2281 tcgccggcga aggcgagatt gtgtttccgg cgcttgtcga ggccatcagg gacggacatg
     2341 ccccatccga catcactcgg gacattccgg gcgtcttctg cagggtcgat ggcataacca
     2401 cgggaagtcg cgccggtatc ctgaccgaag aacaactgga aaccctcggc tttcccgcct
     2461 gggacctgat tgaccgacgg atatacgcca gaatcccgtc aatggccgga accggttgcg
     2521 gcgactacat gaccgtgatg acttccaggg gctgcccttt catgtgcgcc tactgccacc
     2581 agacgctcgg caaaaggttc cgcgcccggt ccgccgaatc ggtgcttgca gagcttgatg
     2641 agctgcatgg caggttcggg tattccgaat tcgaaatcgt cgacgactgc ttcaatctcg
     2701 accgcgaaag gatgagcgcg atactggaag gaatcgcgac gcggcttccc ggcgccaggc
     2761 ttcatttccc gaacggcctg cggtcggacc ggctcgaacc ggccgacatc aagctgatgc
     2821 gtcgcgcggg gacggtgtct gcgtgtttcg ccatcgaaac tgcaacgccg gaattgcaga
     2881 aactgatcgg aaaaaacctc gacatcggga aggcgcgcgc cgccattgaa tgcgcggttt
     2941 cccagggtat cttctcgaca ggcttcttca tgctcgggct gcccaccgaa acgctcgacc
     3001 aggcgaccgc cacgatcgat ttcgcctcga atttaccgat acaccgcgcc atattcatgc
     3061 ttaccacccc cttcccggga acgccgctgg cggatatgac agcgaacatc ctgaaggaac
     3121 gcggcatttc gcaggcaggt gaaaacctga actatttcac ccgcggcgtg aacgtttcgg
     3181 caatgaccga cgacgagctg aatcgcgtat ttcgcgattc gtaccgacgc ttctacctgg
     3241 acccgcgcag ggtcgcccga atccttgcag cacacccccg caagctgtcc ctgccccgct
     3301 acggattgat gaccctggcg aaggttcttc ccggtgtctg gcggaccggt gccgggcgga
     3361 cctgattccc cggcacgacg ttgtcaatgt ggacagtccc gtgccaccgg tctaagatgc
     3421 ggctccgaaa agacggacgg atcaatcgca tggctgaagt gcttctgatt aaggcctggg
     3481 caccgggcgt cgacgacagg gcaatggtcc ctccgatagg cgtgatgcaa ctggccggca
     3541 tactgcgcca ggctggtcat caggtacgcc tgctccatgt cggcccaatc gccgtttccc
     3601 gtgaaaagat cctgaacgcc gtcgccgaca agcgtccaga tgtaataggc atcagcgcaa
     3661 ttaccaccga attcctggtg tatcgcgaaa tcgccggact cctgaacgag tcattccccg
     3721 gaatcccggt aatggcgggc gggccgctgg cgtcttcaaa cccgccggcc gccctcggcg
     3781 ttcccggggt ttcggtgatt gcggtcggtg aagcggacgt aaccattctt gaacttgtcg
     3841 acgctctcgc ggccggcagg gaccctgccg gtctgccggg aaccgccgtc ctgcgcaaca
     3901 acattgtcgt gcgcggcccg aatcgcgaac cgctcacacc ggcggaactg gacgctctgc
     3961 cgctgccggc ctgggacctc ctcgacctgg acgaacactt cagacggcgc ggtatggcgt
     4021 cagtcggtat aaggccatac atgcaggtga tgacgtccag gggatgcccg taccagtgcg
     4081 cgtactgcca cgggctgatg gggcgtaggt acagggccag gtctccggaa tcggtcgtaa
     4141 acgaactgcg cgccctgagg gaatatttcg acatccacga attcgagatc gtcgatgact
     4201 gcttcaatct tgatcgcccg aggatgcatg caatcctcca gggcctgatt gacttcaagg
     4261 accctttgct gcgcctgcag ttcccgggtg gactcaggtc ggacatcctg gaaaaggaag
     4321 acatcctgat gctgcgccgg gccggcacga acttcatttc gttcgcgatt gaaacagctt
     4381 ccccccgcct gcagaaaatg gtccgcaaga acctgaacat cgaacgggct cttgaatcaa
     4441 tttcaacagc cgccgacgcc gggatcttct gcaacggttt cttcatgctc ggcttcccga
     4501 cggaaaccgc ggaagaatgc cgtgcaacca tcgatctcgc ctgctcgacc aggcttcacg
     4561 aggcgctgtt cttcaaggtt aacccgttcc gcgggacgga aatgtttgac atggccgtgc
     4621 agatgtccgg gctggatcca gcggccatcc ctctggagga aatggactac ttcaatgtca
     4681 gacacaacct gtcggccatg cccgacgccg aattcgagcg cctctggaca ggggcctaca
     4741 gaaggttcta tttcaatcct tccaggatca tccggataat cctgcggcac ccgcgaaagc
     4801 ttcagttgct catgtacctg ccccaggtgg ttcgaaagac tgtcaagtcg ctgcttggcg
     4861 gtcatatgcg catgcccggg gcgtcgaagg caggaagaaa cagcggtgac aaggaaaact
     4921 gacgattgaa tctttgcagg gtgggcgata ctgggattga accagtgact tccaccgtgt
     4981 gaaggtggtg tgtaggaact ttcatccaca aaacggctcc atgaggccag tatggctggg
     5041 t
//
LOCUS       DAOPPA010000221         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18388_length_4981_cov_3.163419, whole genome shotgun sequence.
ACCESSION   DAOPPA010000221 DAOPPA010000000
VERSION     DAOPPA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18388_length_4981_cov_3.163419"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /gene="prs"
                     /locus_tag="PKH54_07665"
     CDS             <1..472
                     /gene="prs"
                     /locus_tag="PKH54_07665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOC99806.1"
                     /translation="VMGDTGAAKAFSYYSDELDLPMAIIDKFRSDHTDRPTIRQIVGD
                     VKGKSCLLVDDEIATAGTLVKAAAKLMEFGARRVLATAVHPVLCGDAVQRIIDSPIEK
                     VIIGNTVPVHNKIGAHPDRFSVLDLSGLFAKAIQCIHNGDSMSGLFPPSIRRTN"
     gene            508..1752
                     /gene="thiI"
                     /locus_tag="PKH54_07670"
     CDS             508..1752
                     /gene="thiI"
                     /locus_tag="PKH54_07670"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOC99807.1"
                     /translation="MKTNAILVTFSEVFLKGGRRGWFLSKLKEGIERQLSGAGRFKVR
                     ELHSMLMVISADTRGNDFADFEVDKGIRDALARCFGAVAFHPCLVVPREISRMEQIAA
                     EIAESDMNGVGTFKVDTTRTVKDFPLDSMEINRRLGGVIWEKTKTPVRMKDPDLTVAV
                     RILSNFAAISLEHIRGPGGLPSGSSGRALLMLSGGIDSPVAGYQLMRRGCEIDAVHFD
                     ASPWTPPESREKVEKLASMLAGYQRGMRLFVIPFGDVQAQLRDSAPGRMLVVLYRRMM
                     LRCAAEIAARTGALAIVTGDNIGQVASQTLENMTVIGQATGMQVLRPLLTYDKMDVVD
                     IARKIGTYETSILPFDDCCSLFVPPHPETAAELERVQKIEESFDIPALVSSAVNGAEY
                     MTFGQVSEPADARPAPAPSPVD"
     gene            complement(1738..2115)
                     /locus_tag="PKH54_07675"
     CDS             complement(1738..2115)
                     /locus_tag="PKH54_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99808.1"
                     /translation="MKISFMKWLAPILLSVLAACATEAVVGVVAPASPAGRVGQQQET
                     ASVARGRPAVAATVIESIIQRDRVVVTLDRGTANGIRPGMSGSMANGRPFKVISADER
                     TCRVVVIGLNLRFATGSDALIYR"
     gene            complement(2121..3791)
                     /locus_tag="PKH54_07680"
     CDS             complement(2121..3791)
                     /locus_tag="PKH54_07680"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOC99809.1"
                     /translation="MAPRPTHTKFIFVTGGVVSSIGKGITAASTAAILEARGLKVSLL
                     KMDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFTSATMGRVNSVSSGQIYE
                     TVINRERQGAYLGGTVQVIPHITDEVKARILKAAEGLDVCIIEVGGTVGDIEGLPFLE
                     AIRQMRQQYGVENTAFMHVTYVPFIRTADELKTKPTQHSVNALREIGVQPDIIVCRSE
                     KPMPPEVKRKISLFCSVPAENVIAAVDAQSVYELPIAFKEEGLDQRLCDQLGIWAREA
                     RLADWKRIVEGVRITDPEVTIHVVGKYVDLKESYKSLSEALLHGGIANRCKVRLHFIE
                     AEDLEGDADIDAVLYGGDGILVPGGFGSRGTEGKIRAVEFARTRGVPFLGICLGLQMA
                     TVEYSRNVCGMTDANSTEFMPDSTHPVVDLLPEQRGVTDKGATMRLGAYPCELLPDSL
                     AARLYGATSISERHRHRYEVNNDFVGELVAKGLTISGLHTGERAGQAARLPDGRNGLV
                     EIIELPGHPFFIASQFHPEYKSRPKSPHPLFRGFIEAALANAGSRKAR"
     gene            complement(4204..>4981)
                     /locus_tag="PKH54_07685"
     CDS             complement(4204..>4981)
                     /locus_tag="PKH54_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99810.1"
                     /translation="DAITSVGILLFSKIPDPGSVKFCSIKSLDQLGSCLRDSVVREVD
                     QNLLDPVAKWLPYFREDVAAVVQENFVPMSFYGRFSRGIATGANQFFVLRPSDIRRLG
                     LADGEAVTCISRSNQLKEPVLDDAILSNLAASDAPIYLFSAPGNHSPAAASYIRKGEM
                     LGVSDRYLTRSRNPWYKTESRTPASILLGVFSRGGYKVILNRTDALNLTCYHGFFPNP
                     VGQRHVVRIFLYLLSVAGRRIIRHPSGIMATIWTNSSLEI"
BASE COUNT         1108 a         1440 c         1384 g         1049 t
ORIGIN      
        1 ggtcatgggc gacaccggcg ccgcgaaggc tttcagctat tactccgacg agctggatct
       61 gccgatggcc atcatcgaca agttccgttc cgaccacacg gatcgaccga caatcaggca
      121 gattgtgggc gacgtgaagg gcaaatcctg cctgctggtc gatgatgaaa tcgcgaccgc
      181 cggcacattg gtcaaggcgg cggccaaact gatggaattc ggtgcccgac gcgtgctcgc
      241 gacggcggtt caccctgtcc tgtgcggcga cgcggtacag cgcatcatcg attcgccgat
      301 tgaaaaggtc atcatcggga acaccgtgcc ggttcacaat aagatcgggg cgcatcccga
      361 caggttctct gtgctcgacc tttccggcct gtttgcaaag gcgattcaat gcattcacaa
      421 tggcgactcc atgtcgggcc tgttcccgcc atcgatccgg cggaccaact gacatatctt
      481 tcgacttgac gaatttcagg ggcgactatg aagacgaacg cgattcttgt gacattttcc
      541 gaggtattcc tgaagggagg ccgaaggggc tggttccttt cgaaactgaa ggaaggcatc
      601 gaacggcagc tttccggcgc ggggcggttc aaggttcggg aactgcattc gatgctgatg
      661 gtcatttcgg ccgacaccag gggcaacgat ttcgccgatt tcgaagtcga taaaggcatt
      721 cgcgacgctc ttgccaggtg cttcggcgcg gtcgcgtttc atccgtgcct ggtggttccc
      781 cgcgaaatca gccgcatgga acagattgct gccgaaatcg cggaatccga catgaacggg
      841 gtcggcacgt tcaaggtcga tacgacaagg accgtaaagg actttccgct tgactcgatg
      901 gaaatcaacc gccggcttgg cggggtcata tgggaaaaga cgaagacacc ggttcggatg
      961 aaggatccgg atctgacggt tgccgtaagg attctgtcca acttcgcggc gatatcgctg
     1021 gagcatatcc gcggaccggg cggcttgcct tccggttcat ccggcagggc actcctgatg
     1081 ctgtcaggcg gcatcgacag ccctgttgcc ggctatcaac tgatgaggcg cggatgcgaa
     1141 atcgacgcgg tccattttga tgcatctccg tggacgccgc ccgaatcacg tgaaaaggtc
     1201 gagaagctgg cttcaatgct tgcaggatac cagcgcggca tgcggctgtt cgtgatcccg
     1261 ttcggcgacg tccaggcgca gcttcgcgac tcggcgccgg gacggatgct ggtcgttctc
     1321 tacaggcgga tgatgcttcg atgcgccgcg gagattgcgg ccaggaccgg tgccctggcc
     1381 atcgtaaccg gcgacaacat cggccaggtt gcaagccaga cgctggaaaa catgaccgta
     1441 atcgggcagg cgaccggaat gcaggtgctt cgcccgctgt tgacctacga caagatggac
     1501 gtcgtcgata tcgccaggaa gatcgggacg tatgaaacat cgattcttcc gtttgacgac
     1561 tgttgcagcc ttttcgttcc gccacatccc gaaaccgcgg ctgaactgga aagggttcag
     1621 aagattgagg aatcgtttga tatcccggcc ctggtttcaa gcgctgtcaa cggcgcggag
     1681 tacatgacat tcggacaggt ttccgaacct gcggacgcac ggccggcgcc cgcaccgtca
     1741 cctgtagatt aaggcgtctg acccggtcgc gaatcgaaga ttcaatccaa tcactacaac
     1801 ccggcacgtc ctttcgtccg ccgaaatcac cttgaatggc cgaccgttcg ccatcgagcc
     1861 ggacatgccc ggacgtattc cgttggcggt tcccctgtca agggtaacca cgacacgatc
     1921 acgctggata atcgactcga taacggtcgc cgcgacagca ggtcggcccc gggcgacgga
     1981 agcagtctcc tgctgctggc cgacccggcc tgccggcgag gcgggcgcaa caaccccgac
     2041 gacagcttcg gtggcgcatg ccgcaagtac ggaaagcaga atcggcgcca gccatttcat
     2101 gaatgatatt ttcacggcag tcaacgagcc ttccttgacc cggcattcgc cagtgccgcc
     2161 tcgataaacc cgcgaaacag cgggtgtggc gatttcggcc tggacttgta ctcgggatga
     2221 aactggcttg caataaagaa cgggtgtccg ggaagctcga taatctcgac cagaccgttt
     2281 cgaccatccg gcagcctggc cgcctgacca gccctttcac cggtgtgaag cccggaaatc
     2341 gtcaatccct ttgcgaccag ttccccaaca aaatcgttgt tgacctcgta cctgtggcga
     2401 tggcgttccg atattgacgt cgcgccataa agccttgcgg ccaggctgtc gggcaacagc
     2461 tcgcacggat aggcgccaag cctcatcgtc gcgcccttgt cggtgacgcc gcgctgttcg
     2521 ggaagcagat cgaccaccgg atgcgtgctg tccggcatga attccgtcga attggcgtcc
     2581 gtcatcccgc acacgttccg tgagtactcc accgtcgcca tctggagacc aagacagatt
     2641 ccaaggaacg gcacgcccct tgtcctggca aactcaaccg ccctgatctt gccttccgtt
     2701 ccgcgtgaac cgaagccgcc gggaaccagt attccgtccc cgccgtacag gacggcgtca
     2761 atatcggcgt cgccctcgag atcctcggcc tcgatgaagt gaagcctgac cttgcagcga
     2821 ttggcaatcc cgccgtgcag cagcgcctcg ctcaggctct tgtacgactc cttcaggtca
     2881 acgtacttgc cgaccacatg gatggtgact tcgggatccg tgatgcggac gccttcgaca
     2941 atcctcttcc agtcggccag gcgtgcctct cgggcccaga tgccaagctg gtcgcaaagc
     3001 cgctgatcca ggccctcttc cttgaacgca atcggaagct catatacgga ctgggcatcg
     3061 acggcggcaa tgacgttctc ggcgggcacg ctgcagaaca gcgatatctt gcgcttgacc
     3121 tccgggggca tcggcttttc ggatcgacac acgatgatgt ccggctggac gccgatttcc
     3181 ctcaacgcgt tgacgctgtg ctgcgtcggc ttcgtcttca gttcgtcggc cgtcctgatg
     3241 aacggaacat aggtgacatg catgaaggca gtattttcga ccccgtactg ctgccgcatc
     3301 tggcggatag cctccaggaa cggaagcccc tctatgtcgc caaccgtgcc gccgacctcg
     3361 ataatgcaga cgtcaagccc ctcggccgcc ttcaaaatcc tggccttgac ctcgtcagtg
     3421 atgtgcggga tgacctgaac cgttccgcca agataggcac cctggcgttc gcggttgatc
     3481 acggtttcgt atatctggcc gcttgatacg ctgttgactc ggcccatcgt cgccgacgtg
     3541 aacctctcat aatgccccag gtcgagatcg gtttcggcgc catcgtcggt aacgaagacc
     3601 tcgccgtgct ggaacggatt catcgtgccg ggatcgacat tcaggtaagg atccatcttc
     3661 agaagcgaaa ccttcagccc gcgtgcctca aggatggcgg cggtcgatgc ggcggtgatg
     3721 cccttgccga ttgatgagac gacaccgccg gttacgaata tgaattttgt atgcgttggt
     3781 cgtggggcca tgattcctcc aaacaggttt ggaaaaaaaa aacatggccc agcaggattt
     3841 acttggccaa catatgttaa ccacccatcc ggcaacttgt caaacgccca tcacaacatg
     3901 ctgacattat tgaccaataa tcgccgcgat ctggttgcgg atgattatgg attcatctct
     3961 ggcagaacaa gggcagttct ggcgcggccc cgggactcac tggcaaacct gacggtatgc
     4021 aggacttgat cgcgggcaac gctttttttg aattccgctc cccgtcggaa ataatcaatt
     4081 gggaaaaaaa gatctcaagt ctctcgggta ttctgccaaa ctcaaccaaa tgattgacgg
     4141 catgactaac atcattatct gacaactgat agaaaacttg ttcagaagga accatcgatc
     4201 ggttcaaatc tccaggctcg aatttgtcca gattgtcgcc ataatgcctg acggatgacg
     4261 aatgattcga cgacctgcaa ctgacagaag ataaaggaat attcgaacca catggcgttg
     4321 accgaccgga ttcggaaaga atccgtggta gcaagtcagg ttcaatgcat cagttcgatt
     4381 cagaatgacc ttatatccac ccctggaaaa tactcccaaa agaatgctgg ctggtgttct
     4441 tgactccgtc ttgtaccacg gattcctgga acgggtcagg tatctgtcag aaacgccaag
     4501 catttcgccc ttacggatat atgacgctgc tgccggagaa tggttacctg gggccgagaa
     4561 gagatatatc ggcgcatcgg aagcggccag atttgaaagg atcgcatcat cgagaactgg
     4621 ttctttcagc tgattgctgc gagatataca ggtgacggcc tcaccatccg ccaacccaag
     4681 ccgccggatg tcagaaggtc ttaagacaaa aaattggttt gctcccgtgg caattcccct
     4741 gctgaacctt ccataaaagc tcatcggaac gaagttttcc tgaaccacgg cagcaacatc
     4801 ttccctgaaa tatggaagcc atttcgccac gggatccagc aagttctggt caacttcgcg
     4861 aacgacggaa tcccgaagac aagaaccgag ttgatcaagt gatttgatag aacagaattt
     4921 gacactgccc ggatcgggta tcttcgaaaa cagcaatatt cccactgaag tgattgcatc
     4981 a
//
LOCUS       DAOPPA010000222         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18506_length_4959_cov_3.491436, whole genome shotgun sequence.
ACCESSION   DAOPPA010000222 DAOPPA010000000
VERSION     DAOPPA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18506_length_4959_cov_3.491436"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..876
                     /locus_tag="PKH54_07690"
     CDS             79..876
                     /locus_tag="PKH54_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOC99811.1"
                     /translation="MIDYMAVGLVLFFGILGLVAGTLLQSLRLVASGVSIYVALKYSP
                     VIADSVPVFAKSMMLREYGLPVMLFAVLYMLMILIIKVILNAASPAAARISVPSRLIG
                     GILGAANGLLLAWFIVAVALAAQAGSGRKMVVMDAEKSVFVKFVQDHPVKGLTVRDPA
                     QVEEMKVHIRQMADDAAREVQELTGKAMDQAGKLKETVGEGVGKATDKVEDAAGIVAD
                     KVEGAASDAGEKVEDATGNVGERVRNAADKAGDKVKDAADKVGDKVE"
     gene            873..1601
                     /gene="mrt"
                     /locus_tag="PKH54_07695"
     CDS             873..1601
                     /gene="mrt"
                     /locus_tag="PKH54_07695"
                     /inference="COORDINATES: protein motif:HMM:NF040914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myxosortase family intramembrane protease"
                     /protein_id="HOC99812.1"
                     /translation="MSKPSDLPEPRGIILEVGAWFIVLAILLYIGSILVAVGNFAGDI
                     GGSLPPAFCLIFPVVAAGRRGVTLEAMGLGRMHWGRALAAVGIASIVIFPLFVVGYDV
                     WLKLSGELPGRLLAAYSKAPVISAAWLVNLVFVQVFAVALPEEVFYRGWMQSRLGIVF
                     KTRFRVPGADVGLHVVVTAALFAVSHLVVTPSPARLAVFFPGLLFGYLRERTGSVAAP
                     AIFHALCNIALALVQQFHLRTPPI"
     gene            1755..3125
                     /locus_tag="PKH54_07700"
     CDS             1755..3125
                     /locus_tag="PKH54_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOC99813.1"
                     /translation="MNSTRFHRIKKGLDLPLRGSVGQVVYEGPPVSEVAVIPDDWVDV
                     SARLAVEVGARVRIGDLLFEDRRNPGVRFTSPGSGTVSAVNRGERRQVSSIVVSIDGS
                     REYADFPSYSGRALADLSGDEVRALMVESGLWTALRQRPFSTVPPVDTPAAPIFVTAI
                     DTYPLAPDPVIAISERRGEFLDGLAGLVRLAGGRPVFVCVGDGPGVEGIVDVPGVEVH
                     TFTGPHPAGLPGTHIHFISPASVGAPRWHIGYQDVIALGALLASGRLMVDRVVGVAGP
                     AVTDPVIWRTRLGAAIGPLLVGRLARGENRVISGSPVFGRGVFDIPQSYLGRFHNQVT
                     AIPEDRDRVFLGWLGAGLHRFSVLPIFLSRFLPGADYEINTSTNGSVRAVMPFGMMER
                     VMPLDIMPVQLCRALMMKDVEWATELGALELDPEDLALCTLVCPGKNDYGQALTEVLA
                     MIRKEG"
     gene            3125..4420
                     /locus_tag="PKH54_07705"
     CDS             3125..4420
                     /locus_tag="PKH54_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOC99814.1"
                     /translation="MKAVRRILDMMSGPFHKGGRLHRLYPLYEAIDTFSFTPGKVTTG
                     RTHVRDGADLKRLMITVVVALTPAILWAIFNTGYQANLAIERLAASGGDVGSYALDNW
                     RTSLYLAMGLQFSSASIVGCVVHGLLYYLPVLIVTFLVGGVWEVLFAAVRGHEVNEGF
                     LVTGFLFPMILPPAIPLWQVAMGVSFGIVIGKEVFGGVGKNIFNPALVGRAFLFFAYP
                     AQISGDSVWIAARGLTADGVSGATALAVVKQEGMDALAAAGFNWWDAFIGLVPGSMGE
                     TSALACLIGAAVLLVTRVASWRSMFGVVLGTVAFSAMFNVIGSQTNPAFAMPFWWHMV
                     LGGWAFGTVFMVTDPVSSAFTARGQFIYGFFIGVMAVLIRVINPAYPEGMMLSILFMN
                     MFAPLIDFGFMRANIRRRAKRLAQAAASEANGGDSHAVR"
     gene            4407..>4959
                     /gene="nqrC"
                     /locus_tag="PKH54_07710"
     CDS             4407..>4959
                     /gene="nqrC"
                     /locus_tag="PKH54_07710"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOC99815.1"
                     /translation="MQYGKRYTILFAAAVCGVFAILVTSSAVLLKDRQEENFRLDRMK
                     NVLTVAGLMEPGQKLGRTEIQKIFDENIESRLIELKTGQYVTDGPEPAGYDQRKATAD
                     AATSLNAPDNRAGLVRLPKMGLVYLIKDRQGVRPFSGLLLPIEGKGLWSTLYGYLALE
                     SDLNTIRGITYYQHGETPGLGGEV"
BASE COUNT          819 a         1344 c         1614 g         1182 t
ORIGIN      
        1 gtccttaagt tgcattgggg gaatgatgga aatatccttg gggcgtccgc tggggcgtta
       61 acttgaaggt gccggaaaat gattgactac atggctgtcg ggctggttct gtttttcggg
      121 attcttggcc tggtcgcggg aaccctgctg cagtcgttgc gccttgtcgc atcaggggtt
      181 tcgatttacg tcgcgctgaa gtattcgccg gtcatcgcgg attcggtgcc cgtgtttgcg
      241 aagtcaatga tgttgaggga atatggcctt ccagtcatgc ttttcgccgt cctgtacatg
      301 ctgatgatat tgataataaa ggtcatcctt aacgcggcat ctccggcggc ggccaggatt
      361 tccgtgccaa gtcgcctgat cggcgggatt cttggcgcgg cgaacggcct gctgctggca
      421 tggtttatcg tggccgtggc ccttgcggcg caggccggct ccgggcggaa gatggtcgtc
      481 atggatgctg aaaagtcggt gttcgtgaag ttcgttcagg atcacccggt caaggggctt
      541 acggtcaggg accccgcgca ggtcgaggaa atgaaggttc atatccgcca gatggccgac
      601 gacgcggccc gcgaggttca ggaactgacg ggaaaggcga tggatcaagc cggaaaactg
      661 aaagagaccg ttggcgaagg cgttgggaag gcgacggata aggtcgagga tgctgccggc
      721 atcgttgcgg acaaggtcga aggcgcggcc tcggatgccg gtgagaaggt cgaggacgcc
      781 accgggaatg tcggcgaaag ggtcaggaac gccgcggaca aggccggaga caaggttaag
      841 gatgcggcgg acaaggtcgg ggataaggtt gaatgagcaa gccttctgac cttccggagc
      901 ccaggggtat catcctggaa gtcggcgcct ggttcatcgt tctggcaatc ctgctataca
      961 tcggttcaat ccttgtcgcc gtcgggaatt ttgccggcga tatcggcggg tcgcttccgc
     1021 cagccttctg cctgatcttt cctgtcgtcg cggcgggccg gcgtggcgtg acgcttgaag
     1081 cgatgggcct tggccgcatg cactggggca gggcgctggc ggcagtcggc attgcttcca
     1141 tcgtgatttt tccgcttttc gttgtcggct atgatgtgtg gctgaagctt tccggtgaac
     1201 tcccgggcag gttgctggcc gcgtattcga aggccccggt catttccgcg gcatggctgg
     1261 tgaacctggt tttcgttcag gtcttcgcgg tcgctctgcc ggaagaggta ttttatcgcg
     1321 gctggatgca gtcgcgactg ggcatcgttt tcaagaccag attccgcgtt cctggcgccg
     1381 atgtcggtct tcatgtcgtg gtcacggcag ccttgttcgc cgtttcccat cttgttgtca
     1441 caccgtcgcc ggcgaggctg gcggtgttct ttcccgggct gctgttcggc tatctgcggg
     1501 agcgcacggg atctgtcgct gcgccggcca tctttcacgc gctgtgcaat atcgcgctgg
     1561 cgcttgttca gcagttccat ttaaggacgc ccccgattta gtcttttgcg acgcatggtt
     1621 tcaaacattg ttcttgttta ccccaccgat tcatgcaaca ttttcgaaaa ccgtcaatcg
     1681 acggatgtct ttcgtcatct gttcgtccgt cttccggcgt gccggtcgcg gatcagcttg
     1741 ataagcgggt gtcgatgaac agcaccagat ttcaccgcat caaaaagggg cttgaccttc
     1801 cgcttcgcgg cagtgtgggt caggtcgttt atgagggtcc gcccgtttcg gaagtggctg
     1861 tcattccgga tgactgggtt gacgtgtccg ccaggcttgc cgtcgaggtc ggtgccaggg
     1921 tcaggattgg cgatctgctt ttcgaggacc ggcgcaatcc cggggttcgt ttcacttcgc
     1981 ccggttcggg aacggtttca gcggttaacc gcggggaacg gcgccaggtc tcgtcaatcg
     2041 tcgtatcgat tgatggaagt cgtgaatacg ctgacttccc gtcatattcc gggcgggctc
     2101 tggcggatct ttccggcgat gaagttcgcg ccctgatggt tgagtccggc ttgtggactg
     2161 ctttgcggca gcgtccgttt tccacggttc ccccggtcga tacgcccgct gcgcccattt
     2221 tcgtaacggc aatcgatacc tatccgcttg cccccgatcc ggtgatcgcg atttccgaac
     2281 gacgcggcga gtttcttgat ggtctggccg gcctcgtaag gctggccggt ggtcgcccgg
     2341 tttttgtctg cgtcggtgac gggcccgggg ttgaggggat tgtcgatgtt cccggggtgg
     2401 aagttcacac cttcaccggt ccacatcctg ctggccttcc cggcacacat atccatttca
     2461 tcagcccggc ttccgtcggc gctccgcgtt ggcatatcgg ctaccaggac gtcatcgctc
     2521 tgggggcgct tctggccagc ggccggttga tggtggatcg tgtagtcggg gttgccgggc
     2581 ctgcagttac cgatcccgtc atatggcgaa cccgtcttgg cgcggcgatt gggccccttc
     2641 tggtcggtcg gcttgcaagg ggggaaaatc gcgtgatttc cggttccccg gtctttggac
     2701 ggggcgtttt tgatatcccc cagtcatacc ttggcaggtt ccacaatcag gtcaccgcca
     2761 ttcccgagga tcgtgaccgc gtattcctgg gctggcttgg cgccggcctc cacaggttca
     2821 gcgtgcttcc gattttcctt tcccgctttc tgcctggcgc cgattacgag ataaatacgt
     2881 caacaaacgg aagcgtgcgc gccgtgatgc cattcgggat gatggaacgg gtcatgccgc
     2941 tcgacatcat gccggttcag ctttgccggg cactgatgat gaaggacgtt gaatgggcga
     3001 ccgaactggg cgctcttgaa ctggatccgg aagacctggc tctgtgcacg ctggtctgtc
     3061 ccggtaagaa tgattacggc caggcgctga cagaggtgct tgccatgata cggaaggaag
     3121 gctgatgaag gctgtcaggc ggattctgga catgatgtcg ggaccgttcc acaagggcgg
     3181 ccggctgcat cgcctgtacc cgctgtacga ggcgatcgac actttctcgt ttactcccgg
     3241 caaggtgacc acgggaagaa cacatgtcag ggacggcgcc gacctgaagc gtctgatgat
     3301 caccgtggtc gtcgcgctga cgccggcaat cctgtgggcc atcttcaaca ccggatacca
     3361 ggctaacctg gccatcgagc ggctggccgc atctgggggc gatgtcggca gttacgccct
     3421 ggataactgg cgcacgtcgc tatatctggc gatgggtttg cagttttctt cggcgtcaat
     3481 cgtcggctgt gtggtgcatg ggctgcttta ctaccttccc gttctgatcg tcacattcct
     3541 ggtcggaggc gtctgggaag tcctgttcgc ggccgttcgc gggcacgaag tcaacgaggg
     3601 tttccttgtc acgggcttcc tgttcccgat gatccttccc ccagccatcc cgttgtggca
     3661 ggtcgcgatg ggtgtcagct tcgggattgt catcggcaag gaagtgttcg gcggtgtcgg
     3721 gaagaacatt ttcaatccgg cccttgtcgg acgcgcattc ctgttttttg catatccggc
     3781 ccagatttcc ggtgattccg tctggatcgc ggccaggggg cttactgccg acggtgtcag
     3841 cggggcgacc gcgctggctg tggtgaagca ggaaggaatg gacgcgttgg cagccgccgg
     3901 gttcaactgg tgggacgcct ttatcgggct ggttcccggg tcaatgggcg agacctcggc
     3961 cctggcctgc ctgatcgggg ctgccgtgct ccttgtgacc cgggttgcgt cctggcgttc
     4021 gatgttcggg gtcgtgctcg gaactgtcgc cttttcggcg atgttcaacg tgatcgggtc
     4081 gcaaacgaac ccggctttcg cgatgccttt ctggtggcac atggttcttg gcggctgggc
     4141 gttcggaacg gttttcatgg tgaccgatcc tgtcagcagc gcgttcacgg cccgggggca
     4201 gtttatttat ggattcttca tcggcgtcat ggccgttctg attcgggtga ttaatcccgc
     4261 ctatccggaa ggcatgatgc tgtcgatact gttcatgaac atgttcgccc cgttgattga
     4321 tttcgggttc atgcgggcga atatccggcg cagggccaaa aggcttgccc aggcggccgc
     4381 cagcgaagcg aatggaggtg actcgcatgc agtacggtaa gcgatacacc atccttttcg
     4441 cggccgcggt atgcggcgtg ttcgcgattc tggtcacgtc ctccgcggtt ctgctgaagg
     4501 atcgacagga agaaaatttc aggctggatc ggatgaagaa cgtcctgacg gtcgcgggac
     4561 tgatggagcc gggtcagaag ctgggtcgca cggaaatcca gaagattttt gacgaaaaca
     4621 tcgagtcgcg gttgatcgaa ctgaaaacag gccagtacgt aacagatggc ccggagccgg
     4681 ctggatatga ccagcgcaag gccacggctg acgcggcaac cagcctgaac gccccggata
     4741 acagggcggg actggtacgg ctgccgaaga tgggccttgt ctatctgatc aaggatcggc
     4801 agggcgtgcg gccgttttcg ggccttcttc tgccgatcga gggaaagggc ctgtggtcaa
     4861 ctctttacgg gtaccttgct cttgagtccg atctgaacac gatacggggc atcacctact
     4921 atcagcacgg cgagactcca ggcctgggtg gcgaggtcg
//
LOCUS       DAOPPA010000223         4955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18524_length_4955_cov_3.721020, whole genome shotgun sequence.
ACCESSION   DAOPPA010000223 DAOPPA010000000
VERSION     DAOPPA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18524_length_4955_cov_3.721020"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKH54_07715"
     CDS             complement(<1..902)
                     /locus_tag="PKH54_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99816.1"
                     /translation="MKRPGILLCILVGMAAGCASDPFGTDFSGIEAADVDGIDDATAD
                     SHAGHDHVSDMQTDQTSGDIPATIPPIRLMTFNTGTSEANGKADPDDRYTEWHASQSD
                     EYYGDGLAWMPAIEAAKDFIDAQAPDIIAFQEIFHSAECADIPLESRTDFICTDWRPG
                     DPTVASIITGDDYQVMCNPGKPDKCSAVRKSFGRFRGCDDDFCLEGMTGCTVDTCGKG
                     ARIGRATIELNGGGEINLVSVHASSGFKEDEMQCRAWQFEQIFVDMCGAGPGATGDVS
                     VVMGDFNTDPGRAVIFDPSAARLL"
     gene            complement(975..1850)
                     /locus_tag="PKH54_07720"
     CDS             complement(975..1850)
                     /locus_tag="PKH54_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99817.1"
                     /translation="MKTLSVATAAIAALLSIACSGGAGAEDDLGGMDVRIVPDAALED
                     ARAGDETVGRDEGAGTDTGETDSGSTDTMTLADTTSEDVAPPDTGTPDTTLDQGTVTE
                     VEAYGECNNDAECEGVLDGVPVSMECFAFPDSKYGFCTRGCTKDEDCPSHPSGYTRGC
                     YIEDGDTSGTCLLLCGAVGGGNGCPAHLQCVSSQFCLPPSTTVATKEAGEACTGPAEC
                     LSGECIEGEATRAHCALKCQTNEDCAGPDGLWAGTCTNAGGLNYNFCIFMCGMMGGGK
                     SCPGELECVGMSVCQ"
     gene            2107..2688
                     /gene="msrA"
                     /locus_tag="PKH54_07725"
     CDS             2107..2688
                     /gene="msrA"
                     /locus_tag="PKH54_07725"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOC99818.1"
                     /translation="MKKLVIAFVLLIGTGCAVEDEFADDQLAGEVLASDEGGTDDSQP
                     PAFGVAIVAGGCFWGIEDKLKKVDGVIDTDVGYCGGTVPDVTYEMVSTGTTGHAESVK
                     VLYNPSVITYERLIASFLRTHDPSVVHPLSGYGSQYRSAVFYLTEEEREDAMRAIAAL
                     DKSGLWDDPIVTQVVPAGQFWMAEEYHQDYYDK"
     gene            complement(2692..3381)
                     /locus_tag="PKH54_07730"
     CDS             complement(2692..3381)
                     /locus_tag="PKH54_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC99819.1"
                     /translation="MDKVALLVGNSDGIGLAMTRRLLERGWTVHGISRSPSPVNHASC
                     THLAMSVSDPAYPAEVNRIAATTRPSLCIYCAGIGLKLDVGSMERELSVFDVNLMGMI
                     RTFAAVVPGMIQRNGGHFIGISSLADELNDHGHPSYSASKAGFTRYLESIAPAMRRRG
                     VAVTNVRFGFVRTKMPEGDRLPFIMTVERAADHLERCMSRRPVRYSTPKIMVPMVKLA
                     RVVLFFRHLFG"
     gene            complement(3445..4548)
                     /locus_tag="PKH54_07735"
     CDS             complement(3445..4548)
                     /locus_tag="PKH54_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99820.1"
                     /translation="MMFSKRLLLTTASMIPMLALSSPSVSLERGPALFWGVSAITELP
                     QETVDDVSAWLGGEIGRAAGVRVLDQDAVDEGFDAAVESGDCQENDMPCIVAVARAID
                     AAEAVSADLGKVGGVFVLTIRRIGATSGSVIRQVTGQTDEGIDVLVRALPDLVATLYD
                     IPHNQLGTIRDSRYEAGLSYVIVTYGRPFAWLRPAYHLGSYPWWWAGPVRYHHHHHRW
                     WGWHHRHPDRRPHHPAHRPGRHDGRHDGRHDGRHDGRHDGHGRHDQDRRDRNHDGGDR
                     VRIENPPRAVVPARPEVRQPRDNARGSGITAPARDHRTTRPTVTTAAPSVRDRDIRKD
                     SKKSKKPVEKKSGRGDRKARPGKAAMPGSIPRR"
BASE COUNT          864 a         1531 c         1505 g         1055 t
ORIGIN      
        1 tcaagaagtc tggcagcgct gggatcgaat atgaccgcac gtccgggatc ggtgttgaag
       61 tcgcccatga cgacgctgac atcgcccgtg gcgccaggcc cggcaccgca catatcgacg
      121 aatatctgtt cgaactgcca ggcccggcac tgcatctcgt cttccttgaa tccagacgac
      181 gcatggacgc tgaccaggtt gatttcgcca ccgccattca attcgattgt ggcacggccg
      241 attcttgcgc ccttgccgca tgtatccact gtgcagccgg tcattccttc caggcagaag
      301 tcgtcgtcac agcccctgaa ccggccgaac gatttccgaa cggcggagca tttgtccggc
      361 ttgccggggt tacacatcac ctggtaatca tccccggtga tgatggacgc gaccgtcggg
      421 tcgccaggcc gccagtccgt gcaaatgaag tctgttcttg attccagcgg gatgtcggca
      481 cattcggcgg aatgaaagat ctcctggaag gcgatgatgt ccggcgcctg agcgtcaatg
      541 aaatcctttg cggcctcgat tgccggcatc caggccagac catccccata atactcgtct
      601 gactggctcg catgccactc ggtgtaccgg tcgtccggat ccgccttccc gttggcttcg
      661 gatgtcccgg tattgaaggt catcaagcga atgggcggga tcgttgccgg gatgtccccc
      721 gatgtctgat ccgtctgcat gtcggagacg tggtcatgcc cggcatggga gtcggccgtg
      781 gcatcatcga ttccgtcaac atccgcggct tcaatgcctg aaaagtcggt tccgaacgga
      841 tcggaggcgc atccagccgc catcccgacc aggatgcaca gaagaattcc aggtcttttc
      901 atggtatcgc ccgcaatcga cggacaatca tattacgtta agaggatacg gcagaatatc
      961 cggaatggtt ccagtcactg gcataccgac atgccgacgc actccagttc gccgggacag
     1021 gattttccgc cgcccatcat tccgcacatg aagatgcaga agttgtaatt caggccgccg
     1081 gcgttcgtgc aggttcccgc ccacaggccg tccggcccgg cacaatcctc gttggtctgg
     1141 cacttcagcg cgcagtgcgc cctggtcgcc tcgccctcga tgcactcccc ggagaggcat
     1201 tcggccggtc cagtgcaggc ctcgccggct tccttggttg ccaccgtcgt cgatggtggc
     1261 aggcagaact ggctggaaac gcactgaagg tgggcgggac acccgttgcc gccacctacc
     1321 gctccgcaga gcagcaggca tgtcccggaa gtatcgccat cctcgatgta gcagccgcgg
     1381 gtgtagccag acggatgtga cgggcaatcc tcgtcctttg tgcagcctct cgtgcagaag
     1441 ccgtatttgc tgtctggaaa cgcgaaacat tccatgctga ccggaacccc gtcgaggacg
     1501 ccttcgcact cggcgtcgtt gttgcactcg ccgtaagcct cgacctcggt caccgtcccc
     1561 tggtcaagtg tcgtatcggg agttcccgtg tccggaggag ccacgtcttc cgatgtcgtg
     1621 tccgccagcg tcatcgtatc ggtgctgcca ctgtcggtct cgccggtatc cgtgccggct
     1681 ccctcgtcgc gtccgactgt ctcgtcgccg gccctggcgt cctccagcgc ggcgtccggg
     1741 acgatgcgga cgtccattcc gcccaggtcg tcttcggctc cggcgccccc cgagcatgca
     1801 attgaaagca aagcggcaat ggccgcggtc gccacggaca gtgttttcat gttgttggtt
     1861 ccttctgaat ccgaggtttt attaccagac ggcggcagtc gtttcaatag aaattgacag
     1921 cttctgtcag tttctttggc ggatgattgc ggctttgggc ggtcttgacg ttccggttgt
     1981 ccgcgaagtg atgtcgtcgg caccgtaacg tgccgtttta caaagcgtat ttgtttacaa
     2041 tgcggcgtcc ggccgcgtcg aaccttagta ttcaatcaag gtctttccgg cttgcggagc
     2101 tgccgaatga aaaagctggt gattgcattt gtgctgttga ttggaaccgg gtgtgccgtt
     2161 gaagacgaat ttgccgacga ccagttggct ggcgaagtgc tcgcttccga cgagggcggc
     2221 acggacgact cccagcctcc ggcgttcggg gtggccatcg tggccggtgg ctgtttctgg
     2281 ggtatcgagg acaagctcaa gaaggttgat ggcgtaatcg ataccgacgt cgggtattgc
     2341 gggggaaccg tgccggacgt cacatacgag atggtctcaa ccggtacgac cgggcatgcg
     2401 gaatcggtca aagttcttta caacccgagt gtcattactt acgaacggct gatcgcgagt
     2461 ttccttcgaa cccatgatcc ctccgtcgtg catccgttgt cggggtacgg ttcccagtac
     2521 cgttcggccg tcttctatct gacggaagag gagcgggaag acgccatgcg cgcgattgcg
     2581 gcattggaca agtccgggct ctgggacgac ccgatcgtga cccaggtggt gccggccgga
     2641 cagttctgga tggccgagga gtatcaccag gactattacg acaagtagca atcagccgaa
     2701 caggtggcgg aagaacagga cgaccctggc cagcttgacc atcggaacca ttatcttcgg
     2761 cgtcgaatag cgtaccggcc gcctggacat gcacctttca aggtggtcgg cggccctttc
     2821 cacggtcatg atgaacggca gccggtcgcc ttccggcatt ttggttcgga cgaatccgaa
     2881 gcgcacgttc gtcacggcca ccccccttct tcgcatggcc ggcgcgatgc tttccaggta
     2941 gcgcgtgaat cccgccttcg aggccgagta cgatgggtgt ccgtggtcgt tgagttcgtc
     3001 cgccaggctg gaaatgccga tgaagtggcc gccattcctc tgaatcattc cggggacgac
     3061 ggcggcgaac gtccggatca tccccatcag gttgacgtcg aagaccgata attcccgctc
     3121 catgcttccg acatcaagct tcaaaccgat tccagcgcag tagatgcaca gtgacgggcg
     3181 cgtcgttgcg gcgatccggt taacctccgc gggatatgcc ggatccgaga cgctcatcgc
     3241 caggtgagtg caggaagcgt ggttgacggg cgacgggctt cgtgagatgc cgtgtaccgt
     3301 ccagccgcgt tccagcagcc ttcgggtcat cgccagtcct atcccgtccg agttaccgac
     3361 cagaagcgcg accttgtcca tggccagacc atttcaagag ggcctgattg taccttcaaa
     3421 tgagtggagc ctgttatccg gatgtcagcg gcggggaatc gaaccgggca tggcggcctt
     3481 tcccggcctg gctttcctgt cgccccgccc cgacttcttt tcaaccggtt tcttggactt
     3541 ttttgaatct ttcctgatgt ccctgtcccg tacggaaggt gcggccgtgg tcacggtcgg
     3601 acgggtcgtc cggtgatcac gggcaggcgc cgtgatgccg gatccgcgcg cgttgtcgcg
     3661 gggctgcctg acttccggac gagccggaac aaccgcccgg ggtggattct cgatcctgac
     3721 gcggtcgccg ccgtcgtggt tgcgatcccg ccgatcctga tcgtgccttc catgtccgtc
     3781 gtggcgtccg tcgtggcggc catcgtggcg cccgtcatgg cgtccatcgt ggcggcccgg
     3841 gcggtgggcc ggatggtgcg gccgccgatc cgggtgcctg tggtgccatc cccaccatct
     3901 gtggtgatgg tggtggtatc tgacgggccc ggcccaccac cagggatagc tgccaaggtg
     3961 ataggccggc ctgagccatg caaacggccg tccataggtg acgatcacgt agctaagtcc
     4021 cgcctcgtat cggctgtccc tgatggttcc caactggtta tgcgggatgt catacaaggt
     4081 ggcgaccagg tctggcagcg cgcgcacaag gacgtcgatt ccctcatcgg tctggccggt
     4141 tacctgcctg attaccgaac cgctggtcgc gccgattctc ctgattgtaa gaacgaacac
     4201 gccgccgact tttcccagat cagcgctgac cgcctcggcc gcgtcgattg ccctggcaac
     4261 ggcaacgatg cacggcatgt cgttttcctg gcaatccccg ctttcgaccg cggcatcgaa
     4321 cccttcatcg acagcgtcct gatccagaac cctgacaccc gcggcgcgcc cgatttcccc
     4381 gcccagccag gctgacacgt cgtcaacggt ttcctgcggc aactccgtga tcgcggaaac
     4441 accccagaac agggctggcc ccctttccag ggagactgac ggacttgaaa gcgcaagcat
     4501 tggaatcatc gaggccgtcg tcagcaacag tctttttgaa aacatcatga ttctctccct
     4561 gtttttgtgg ccggccggtg gcctgtcgcc gccgatccgg cacgggacag attctatggg
     4621 ttcgcaggca gccaggccgc cttgtcggga aaaaaaattg tactgaagat gtatccggcc
     4681 cgtctatctg cgaccgaagc cgccccgccc acgggtcggc ctggaaggct ttgaacgttt
     4741 tcccggggcc gcctgggacg gccgtttctt cccgttgccg gactgcacgc tccccgacgg
     4801 gtactggcgc gggccggggc gatccggcat gacgtgcctg ggcgggggca tgtttcgttc
     4861 cggtcgtaca acaggacgtt cgcgccgcgg atccggcctg tccggcatga cgtgcctggg
     4921 cgggggcatg tttcgttccg gtcgtacaac aggac
//
LOCUS       DAOPPA010000224         4951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18542_length_4951_cov_3.944444, whole genome shotgun sequence.
ACCESSION   DAOPPA010000224 DAOPPA010000000
VERSION     DAOPPA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18542_length_4951_cov_3.944444"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKH54_07740"
     CDS             <1..782
                     /locus_tag="PKH54_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC99821.1"
                     /translation="FVDAYGEDRNSNPYFPDLIPYLAVSGKAGTKWFKIPGGVFNMGS
                     ADGENDEKPVRRVNVRAFEMSRTEVTVWQYRQCVQAGACTAPNTGSGCNWDVADRQDH
                     PVNCVDWEQAKKYASFIGGRLPTEAEWEYAARSGGKDRKYPWGDQEATCDRAVMSHGG
                     NGCGKGGTWPVCSKPEGNTEQGLCDMAGNVWEWVSDKYHDSYNGAPNDGSSWEGAGSY
                     RVDRGGSWRSGAGRVRAANRDGSAPGNRLDALGFRVARSLP"
     gene            961..1491
                     /locus_tag="PKH54_07745"
     CDS             961..1491
                     /locus_tag="PKH54_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOC99822.1"
                     /translation="MKVKVFTINFDPVAGVMDDINLQDFCETHSVVSLQSNFFQMNAR
                     PMLILCLGYSDVEVKPEAGFEPDRNVVHPKEKKKHPKADLDLQQQALYELLRNWRNQT
                     AVARGVPPYLIMDNRQISDICRICPTTLSAFEQIKGLGAGRSNSYGKEIIEIIRQFGS
                     ELTIGGGQDDGKNDGK"
     gene            1466..1846
                     /locus_tag="PKH54_07750"
     CDS             1466..1846
                     /locus_tag="PKH54_07750"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOC99823.1"
                     /translation="MMARTTENEIAALLKWEQVISDIFVRTEKFPKTLRHSLVSRIEN
                     TSLDVLDLIVVAKYSSGQAKVESLNRANIALTRLQVLVRLAHSLAALGNSGYEAISRG
                     IVETGSMLGGWRKSVQANPGQAID"
     gene            1957..2859
                     /locus_tag="PKH54_07755"
     CDS             1957..2859
                     /locus_tag="PKH54_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HOC99824.1"
                     /translation="MGDIENQIFDLQRDLQSWSYHPQAYRTFRVFEPKARIISAAPFR
                     DRVVHHALCATINPVLERYLTDDTYACRVGKGTVAAIRRAQLFCRRFDRFVKLDVRHF
                     FETADHAVMKATLRRLIKDKDLLWLSDLFIDHGAPGSPIGKGLPIGNLTSQNFANLYL
                     GPLDHYVREVLHIPGYVRYMDDMLLFDSDKTHLRAAVKSTSEFLRGLKLEIKHHATRQ
                     GLCSDGVPFLGFNIYPSLIRFDRIRRRRFARKVNNVVLRLNSGQLDQDSAGQILCSVF
                     GWAGLGDTAAFTRSIVQKYEVSGI"
     gene            2921..3076
                     /locus_tag="PKH54_07760"
     CDS             2921..3076
                     /locus_tag="PKH54_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99825.1"
                     /translation="MDDATGSNRVNRGGSWNNDARNVRAANRDNNDPGNRNDNLGFRV
                     ASSGHVR"
     gene            3347..4615
                     /gene="lhgO"
                     /locus_tag="PKH54_07765"
     CDS             3347..4615
                     /gene="lhgO"
                     /locus_tag="PKH54_07765"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOC99826.1"
                     /translation="MPVGTDRSGIYDVVVIGGGIVGTATAMALLQGGRLKVAVLEAES
                     EQAFHQTGHNSGVIHSGIYYKPGSLKAKNCVVGRELLYRFLEENGIPHERCGKLIVAK
                     DEEELGRLDALVERGRANGIEGIARVGRDEIGRIEPAVCSPGGVFVPVTGIVNYTEVT
                     RAYGRVIKSLGGTIHLNTTVNGVTSAKDGLQKVVTPRGDFLARLAINCAGLQSDRVAK
                     MFGVVTDLRIVPFKGEYYDVVPGRSHLVRNLVYPVPDPAFPFLGVHFTRSIEGRVHAG
                     PNAIPAARRDAYRPFAFSGQDFSDMVFFPGFIRMGMRNFGMGVGEMWRSMFKSAFVRD
                     LRRMIPEIRGRDLVPGVPGVRAQAMDRQGNLVDDFAILEMPGSIHVLNAPSPAATASI
                     SIGRTIANRAFERLGVGIRQDWVIQEGGNP"
     gene            4678..>4951
                     /locus_tag="PKH54_07770"
     CDS             4678..>4951
                     /locus_tag="PKH54_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99827.1"
                     /translation="MPAVFPLRCLMVVVTTVAALTAGLAGCGGSVVTPDVAEPVDVVD
                     VAELPPPPSSFVIMTFNVGTTDNMDHTYDEDGYDNRLAEINAGHFGN"
BASE COUNT         1064 a         1255 c         1491 g         1141 t
ORIGIN      
        1 cattcgtcga tgcttatggg gaagacagga attccaatcc gtactttcct gatctgattc
       61 catatttggc cgtttcggga aaggccggaa ccaagtggtt caagattcct ggcggtgttt
      121 ttaacatggg ctcggccgac ggtgaaaatg acgagaagcc ggttcgccgg gtcaatgtgc
      181 gggcgtttga aatgtcccgg accgaagtga cggtgtggca gtatcgccag tgtgtccagg
      241 caggcgcgtg cacggcgccg aataccggtt ccggttgcaa ctgggacgtg gccgacaggc
      301 aggatcaccc ggtcaactgc gttgactggg aacaggcaaa aaaatatgcg tcgtttatcg
      361 gtggtcggtt gccgaccgag gcggaatggg aatatgccgc aagaagtggt ggaaaggatc
      421 gcaagtatcc gtggggcgac caggaggcaa cctgcgatcg tgccgtaatg agtcacggcg
      481 gaaacggttg tggcaagggg ggcacatggc cggtctgttc taaaccggaa gggaataccg
      541 aacaaggctt gtgcgacatg gccggcaacg tgtgggaatg ggtatcggac aagtatcacg
      601 attcgtataa cggcgccccg aacgatggtt catcctggga aggcgcaggc tcgtaccggg
      661 ttgatcgtgg cgggtcgtgg cgcagtggcg ccgggcgcgt ccgtgccgcc aatcgcgacg
      721 gcagcgcccc aggcaaccgc ctcgacgccc tcggcttccg cgtcgcgagg tccttgcctt
      781 gatttccctt tattcttttt cccttttacc cttttcgttc ggaatttccg gaggatgtta
      841 tgcgggccgt gtacgggctg gcttgccaaa gcccgttcat acggcccgca ggcaaaatgt
      901 ttgacgtcag gtttcgaact cgacgcggcc cttgggccgc gaaaattttg cagtgggttt
      961 atgaaggtca aagttttcac gatcaatttt gatcctgtgg ccggcgtcat ggatgacata
     1021 aatctgcagg atttttgtga aacccattcg gtggtttccc tgcaaagtaa tttttttcag
     1081 atgaacgctc ggccgatgtt gattttgtgt ctcggatact cggatgttga ggtcaagcct
     1141 gaagcgggtt tcgagcctga tcgtaatgtc gtccatccga aggaaaaaaa gaagcatcca
     1201 aaggccgatt tggacttgca gcagcaagcc ctgtacgaat tgcttcgtaa ctggcgaaat
     1261 caaaccgcgg ttgcgcgtgg agttccacca tatctgataa tggataaccg tcagatttcg
     1321 gatatttgtc gaatctgtcc gacgacgctt tcggcttttg agcagatcaa gggtttgggg
     1381 gctggtcgtt caaacagtta tggaaaagaa atcatcgaga taatcagaca gttcggctct
     1441 gagttaacga ttggtggggg tcaggatgat ggcaagaacg acggaaaatg aaattgcggc
     1501 tttactgaag tgggaacagg tcatatccga tatttttgtc aggacggaaa agttcccgaa
     1561 aacattacgc cattcccttg tatccagaat tgaaaacacc agtctggacg ttctggatct
     1621 gattgtcgtt gccaagtatt cttccgggca agccaaggtt gaaagtctta accgtgcaaa
     1681 catcgcgctt acacgtcttc aggttctggt aaggcttgca cattcgctgg cggctcttgg
     1741 caactccgga tacgaagcca tttcaagggg gattgttgaa acgggaagca tgctgggtgg
     1801 ctggcgaaag tccgttcagg cgaaccctgg acaagcgatt gattgaagtt gaagcgactt
     1861 ggaaatcaat tcaaatcgat gatttcattt ccgcaccttc ttgacgcggc aattcgtgcc
     1921 gcgaagggga cgcgatcaat ctcggccgcg gagttcatgg gcgatatcga aaatcagata
     1981 ttcgatcttc aacgggattt gcaatcatgg tcgtatcacc cccaagccta tagaactttc
     2041 agagtttttg aacccaaggc gcggataatc agtgccgcgc ctttccgtga cagggtggtt
     2101 catcacgccc tgtgtgcaac aatcaacccc gtccttgaaa gatatctgac cgatgacacg
     2161 tacgcatgtc gcgtcggtaa gggaacggtt gcggccatca gacgggctca gcttttttgc
     2221 aggcgttttg acagatttgt gaaacttgat gtgcgtcatt tttttgaaac ggcggatcac
     2281 gcagtaatga aagcgacgct cagaaggctg ataaaagaca aagatttact ctggttgtcg
     2341 gatttattca tagatcacgg cgcccccggt tctccgattg gcaaggggtt gccgataggc
     2401 aatttgacaa gtcagaattt tgccaatctc tatctcggcc cactggatca ttacgtcagg
     2461 gaagtcctgc atattcctgg ttatgttcgg tacatggatg acatgctttt gttcgattcg
     2521 gacaagactc atcttcgggc ggcagttaaa tcgacttccg agttccttcg cgggctaaaa
     2581 cttgaaatca aacaccatgc cactcgacag ggattgtgca gcgatggggt tcctttcctg
     2641 ggcttcaaca tctatccgtc gctgataagg tttgatcgca tcaggcggcg tcgttttgca
     2701 cgcaaagtga ataatgtggt attgcgattg aattccggac aacttgatca agactcggcc
     2761 ggccagattc tttgcagcgt gttcggttgg gcaggccttg gcgacacagc cgcctttacg
     2821 cgctccattg ttcaaaaata cgaagtatcg gggatttgat gccgggcctt gcagtgaacg
     2881 gttgaatatt tgtttgaggc gttgtcgaat accgataacg gtggatgacg ctacaggctc
     2941 gaaccgggtt aatcgtggcg ggtcgtggaa caatgacgcc aggaacgtcc gtgccgccaa
     3001 tcgcgacaac aacgacccag gcaaccgcaa cgacaacctc ggcttccgcg tcgcgagctc
     3061 cgggcatgtc cggtgagtcg gacagtcagc aagccggcgc ctgatggatt cattttccat
     3121 cgtcgtcggc tccgtaccaa ggcctggacc agttgtcatc aacctgtgtc ccgacgcttg
     3181 caggcaggct gaagggaacg aacagtgggt gggttgtccc ggctggtagc gtccgcttca
     3241 attcggtggc gaacgtcggg acaaccacgc gctgtatcca gtcgggttgg atagaaacgt
     3301 tagtattgtg ctttgtgaac gtacatacgg aataagggtg ggttcaatgc cagtcgggac
     3361 ggacagatcc gggatatatg atgtggttgt tatcggtggc gggattgtcg ggacggcgac
     3421 cgcgatggcc cttttgcagg gcgggcggtt gaaggtcgcg gtgctggagg ccgaaagcga
     3481 acaggccttt caccagaccg gccacaacag cggcgtcata cattcgggaa tctattacaa
     3541 gccgggcagt ctgaaggcaa aaaactgcgt cgtcggccgt gaactgcttt accgattcct
     3601 ggaagaaaac ggcatcccgc acgaacgttg cggcaagttg attgtcgcga aggatgaaga
     3661 agaacttggc cggctggatg ccctggtgga acgcgggcgc gcaaacggca tcgaggggat
     3721 tgccagggtc ggccgtgacg aaatcggccg catcgagccc gccgtatgca gtcccggcgg
     3781 cgttttcgtc ccggtgaccg gaatcgtgaa ttacaccgag gtcacaaggg cctatggccg
     3841 cgtaatcaag tcgcttggcg gcacgataca cctgaacacg actgtcaacg gcgttacgtc
     3901 cgcgaaggat ggcctgcaaa aggtcgtgac gccaaggggc gacttccttg ccaggcttgc
     3961 aatcaactgc gccggtcttc aaagcgaccg cgtggcgaag atgttcgggg ttgtaaccga
     4021 cctgcgcatc gtgcccttca agggcgagta ttacgatgtc gttcccggtc gttcccacct
     4081 ggtgcgcaac cttgtgtatc cggttccgga tccggcattt ccgttccttg gcgtccattt
     4141 cacgcggtcg atcgagggcc gcgtccacgc cggtccgaac gccattccgg cggcccgtcg
     4201 cgatgcttac cggccgtttg cattttcggg ccaggacttt tcggacatgg tttttttccc
     4261 cgggttcata cgcatgggaa tgcgtaactt cgggatgggc gtcggcgaga tgtggcgctc
     4321 gatgttcaag tccgcgtttg ttcgcgacct gcgccggatg attccggaaa tccgcggccg
     4381 cgatctggtg ccgggcgtgc ccggcgtacg cgcccaggcg atggacaggc agggaaacct
     4441 tgtcgatgac ttcgcgatcc ttgaaatgcc cgggtcgatc cacgtcctga acgcgccttc
     4501 gcccgccgcg acggcgtcga taagcatcgg caggacgatt gcgaacaggg cctttgaaag
     4561 gcttggggtc ggaattcggc aggactgggt catccaggaa gggggcaatc catgatgacg
     4621 gtcgtgttcc ggcccgggaa tgggggatca aatcgtcctt ccaggtggca atccatcatg
     4681 ccggccgttt tcccgttacg gtgcctgatg gttgttgtca cgacagtcgc ggctttgacc
     4741 gctggcctgg cgggatgcgg cggatccgtc gtcacccccg acgtggccga acccgtcgat
     4801 gtcgtggacg ttgccgagct tccgccgccg ccttcttcgt ttgtgatcat gacgttcaat
     4861 gtcggcacga ccgacaacat ggatcacacc tacgacgaag acggatacga caaccggctg
     4921 gcggaaatca acgccggaca cttcggcaac a
//
LOCUS       DAOPPA010000225         4923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18677_length_4923_cov_4.356615, whole genome shotgun sequence.
ACCESSION   DAOPPA010000225 DAOPPA010000000
VERSION     DAOPPA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18677_length_4923_cov_4.356615"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PKH54_07775"
     CDS             <1..1210
                     /locus_tag="PKH54_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOC99828.1"
                     /translation="PEFQPDFGPAAFNARSGATVIGAREFLIAYNININSRDKVKASR
                     MAIKMRESDGPARGADGEKILGPDGEWFRVPGALKHIRAVGWTIDEYGIAQVSVNVLN
                     YRKTPLHVVYEEARRHAADLGVLVTGSEIVGLVPKDCLIASGRYYLERQGISPAAPDR
                     ELVHIACRSLGLSDVKVFEPDDKIIEYKLNGGSRTLADMTVSNFCDEVSADSAVPGGG
                     SVAALAGSLGAALAAMVGNLSYRKPELLAVRDELAALAIEAQEVKSSLMGLVVEDSLA
                     FTEVMEAMRLPKATDDEKAVRSAAIARASEHATMVPFRTLKLCCRAAELAGRMATIGY
                     VQCLSDGGTGAAMALAGAEGAAMNVLINLASGKDSPAKQETRAAALEMLERTRASTAA
                     TLAAIHASFK"
     gene            complement(1216..2376)
                     /locus_tag="PKH54_07780"
     CDS             complement(1216..2376)
                     /locus_tag="PKH54_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99829.1"
                     /translation="MSDNLNASELLGLIDSVFCPAADERLLMIMVDLPDSVVVDDRAW
                     AERRETACEWARTIAADSRYEVIFAAYRNPRMNNADLPAGAWLLSPDALPPTADELPE
                     CTQVAFEEIFARRPMILAPTEFSATAPLKMAARRHGFRGATMPGFCRQMVDALRLDYA
                     EVNRRVEMMASLLDVATGATFHFRVDGAAGAQPLEMKLFLDLRHRAAHRSGGRFPSPG
                     QVGNLPSGEAYIVPYEGELPGDPSASAGMLPVQFGSEVVIFRIENNVAVEASGEGPAA
                     VAERARLAAEPAYGNMAELGVGVLGDFGIKPLGEILIDEKLGLHIAFGRSDHFGGQVG
                     PARFSSPAAVVHIDRVYIPEMQPMVHVVRAALQFEGRPDVDLVRDDVPVFAF"
     gene            2499..4538
                     /locus_tag="PKH54_07785"
     CDS             2499..4538
                     /locus_tag="PKH54_07785"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99830.1"
                     /translation="MRNRKGWEAVCHRFTNMIVLVPTMLMVAVVSGACTDVAPSVEDV
                     RIVDEVTIPSNDAESGRNDSGTTEPGGDANQGEDEGGLEGHDVMAEDEGGSSGGKDSG
                     GATGDEGSVSPGGDVMSIEDDIPEDLDCPGAAGCPCQDRDECLSNFCVPTRQGHRCTR
                     MCSGGSQCSSDELCSIFRGDNGADTIYVCLDRWMTACRPCVQDIDCNVPWLDDKLQPF
                     CIPDGQGSRHCAPRVANPAKPECPAGMIPEPRTVHDLEVTLCVPEDGKCPCLQEYLRS
                     GYETECSVTNEFGTCSSRHLCDEECAAAVPAPEVCDAFDNDCDGQIDEELSGIECDTV
                     SPFGSCKGQLSCVNGVGICLGRLASADTCNGIDDDCDGETDEGLGTVSCGTGVCRRQI
                     ESCQNGAAGVCDPYAGRKTEVCDGLDNDCDGLTDEDIPTVTCGKGECRRTVQGCVNGK
                     PAQCTPLSPQNEVCDLRDNDCDGSTDEGFGPVTCGLGSCSHTVMSCVNGVKQACDPFQ
                     GAVDELCNGLDDDCDGKSDDGLWMPENLHEINSTCAVAKDLGIITEGEPLKTFQSYIG
                     KAGDIDWFNFRADEHSHSCALGMDQDYKIYATLFNPAGPEVCMPIVMELYDDKCRLVA
                     SAVDSDCSSHRIEFKWDGECGPDDSRDFRMKIWAFNKEQYSCMPYTLQLYMRKVN"
     gene            4673..>4923
                     /locus_tag="PKH54_07790"
     CDS             4673..>4923
                     /locus_tag="PKH54_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOC99831.1"
                     /translation="MPSCPSEHIKPEDLLVETLGECRFPSPLGAKYFVNDEDRISLTP
                     YMRKLTDRIGRNETMPSFEPAGPRERIFFEPSEVTAGIVT"
BASE COUNT         1076 a         1533 c         1490 g          824 t
ORIGIN      
        1 gccggaattc cagccggatt tcggcccggc ggcattcaac gcccgaagcg gggcgaccgt
       61 catcggagcc cgcgagttcc tgatagccta caacatcaac atcaacagcc gcgacaaggt
      121 caaggccagc cggatggcca tcaagatgcg cgaaagcgac ggtcccgcga ggggtgccga
      181 cggcgaaaag atccttgggc cggacggcga atggttccgt gttcccgggg cgctcaagca
      241 tattcgagcg gtcggatgga ccatcgatga atacggcatc gcgcaggttt cggtaaacgt
      301 gctgaactac cgcaagacgc cgcttcacgt cgtctatgag gaggccaggc gccacgccgc
      361 cgatctcggc gtgctggtaa caggaagcga gatcgtcggt cttgtgccga aagactgcct
      421 gatcgcctcg ggcaggtact atctcgagcg tcaggggatt tcccccgcgg cccccgacag
      481 ggagcttgtc cacattgcat gccggtcgct cggcctgtcg gacgtcaaag ttttcgagcc
      541 ggacgacaag atcatcgaat acaaactgaa cggcggctcc aggacgcttg ccgacatgac
      601 cgtttcgaac ttctgcgacg aagtcagcgc cgattccgcc gttcccggcg gtggctccgt
      661 ggccgcgctt gccggctccc ttggcgcggc ccttgccgcg atggtcggca atctctcgta
      721 ccgcaagccg gaactcctgg cggtcaggga cgaactggcc gctcttgcca ttgaggccca
      781 ggaagtgaag tcatcgctga tgggtctggt cgtcgaggac agcctggcat ttaccgaagt
      841 catggaagcc atgcgcctgc caaaggccac cgacgacgaa aaggctgtcc gttccgcggc
      901 aatcgccagg gcctccgaac acgcaacgat ggtgcccttc cggaccttga agctgtgctg
      961 ccgagccgcc gaactggccg ggcggatggc gacgatcgga tacgtccagt gtctttcgga
     1021 cggcggaacc ggggccgcga tggcccttgc cggtgccgaa ggcgcggcca tgaacgttct
     1081 tattaacctt gcttcgggaa aggattcgcc ggcaaagcag gaaaccaggg cagcagcgct
     1141 ggaaatgctt gaacgtacca gggcttcgac agccgccacc cttgccgcaa tacatgccag
     1201 cttcaaatag tggcgtcaga acgcaaatac cgggacatcg tcgcggacaa gatcaacgtc
     1261 cggccttcct tcgaattgaa gcgccgcacg aaccacgtgc accatcggct gcatttccgg
     1321 gatatagacg cgatctatgt gaaccaccgc cgccggtgac gaaaaccgcg ccggaccaac
     1381 ctggccgccg aaatgatcgc ttcgcccgaa cgcgatgtga aggcccagtt tctcgtcgat
     1441 caggatttcc ccaagcggct tgatgccgaa gtcacccaga acgcccacgc caagttccgc
     1501 catattgccg taagccggct cggcggcaag tctggctcgt tcggccacgg cggccggacc
     1561 ttcacctgaa gcctccaccg ccacgttgtt ttcgatgcgg aagataacga cctcgcttcc
     1621 gaactgaacc ggaagcatgc cggcactcgc gctgggatcg cctggaagtt cgccctcgta
     1681 tggaacgatg tacgcctcgc cggaaggaag gttgccgacc tgtccggggc tcgggaagcg
     1741 accgcccgaa cgatgggcgg cacgatgccg aagatcaagg aacagcttca tttcaagcgg
     1801 ctgcgccccc gccgccccat ctacccgaaa atgaaaggtt gctcccgtgg ccacgtcaag
     1861 aagcgacgcc atcatctcga ccctgcggtt cacctcggcg taatcaaggc gcagcgcatc
     1921 caccatctgc ctgcagaagc ccggcatcgt cgcaccgcgg aagccatgac gccttgctgc
     1981 catcttcaag ggggcggtcg ccgaaaactc ggttggagca aggatcatgg gacgccgcgc
     2041 gaatatctcc tcgaaggcga cctgagtgca ttcgggaagc tcgtcggccg ttggcggcag
     2101 cgcgtcgggt gacagcagcc aggcaccggc cggcaaatcc gcgttgttca tgcgcgggtt
     2161 gcgatacgcg gcaaaaatca cctcatatct tgaatcggcg gcgatcgtcc tggcccactc
     2221 acaagcggtc tcgcgccttt cggcccaggc gcggtcatcc acgacgacgg aatccggcag
     2281 atcgaccatg atcatcagca gacgctcgtc ggccgcagga caaaaaaccg aatcaattaa
     2341 acccaacaat tccgaagcgt taagattgtc agacatgaaa cccccgttga aatgcgcaaa
     2401 aaatcttcct tcacaagtgt gacacattgc gacttcgaac gatacagcac agcggaaccg
     2461 gtccaccaac cacaaaacac ttaaccaagg agaacgccat gagaaacaga aagggatggg
     2521 aagccgtgtg tcacaggttc acgaacatga tcgtcctggt gccaaccatg ttgatggtgg
     2581 ctgtcgtgtc cggagcgtgt accgacgtgg ccccctccgt cgaggacgtc aggatcgtcg
     2641 atgaagtgac gattccgtcg aacgacgctg aaagcgggcg gaacgattcc ggcacgaccg
     2701 aaccgggcgg tgacgccaat cagggcgagg acgaaggtgg cttggaaggc cacgacgtca
     2761 tggccgaaga cgagggcgga tcgtcgggtg gcaaggacag cgggggcgcc accggcgacg
     2821 aaggaagcgt aagccccggc ggcgatgtca tgtcgattga ggacgacatc cccgaggatc
     2881 tggactgccc cggcgcggcg ggatgcccct gccaggatcg cgacgaatgc ctgtcgaatt
     2941 tctgcgtgcc caccaggcag ggacatcgct gcaccagaat gtgctcgggc ggaagccagt
     3001 gctcatccga cgaattatgt tccatcttcc gcggggacaa tggagccgac acgatctacg
     3061 tttgcctgga cagatggatg acggcgtgca ggccgtgcgt gcaggacatc gactgcaacg
     3121 tgccctggct tgacgacaag cttcagccgt tctgcattcc ggacggccag ggttcaagac
     3181 actgcgcgcc ccgggtggcg aacccggcca agccggagtg tcccgcggga atgattccgg
     3241 aaccgcggac agttcacgac ctggaagtca cgctgtgtgt cccggaagat ggcaagtgcc
     3301 cctgcctgca ggaatacctg cgatccggat acgagacgga gtgcagcgtt acgaacgaat
     3361 tcggcacttg ctccagccgc cacctttgcg acgaggaatg cgcggcggcg gtgccggcac
     3421 cggaagtctg cgacgccttc gacaacgact gcgacggcca gattgacgag gaactctccg
     3481 gaatcgaatg cgacacggtc agtcctttcg gatcatgcaa gggccagctc agttgcgtca
     3541 atggggtcgg aatctgcctt gggcgcctgg catcggccga cacgtgcaac ggaatcgacg
     3601 acgactgcga cggcgaaacc gacgagggac tcggcaccgt ctcctgcgga accggagtgt
     3661 gcaggcgaca gatcgaatcc tgccagaatg gcgccgcggg cgtctgcgat ccttatgcgg
     3721 gaaggaagac cgaagtctgc gacggactcg acaacgactg tgacggcctg accgacgaag
     3781 acatcccgac agtcacctgc ggcaagggcg aatgcaggcg gacagtacag ggctgcgtca
     3841 acggaaagcc ggcccagtgc actccgctga gcccgcagaa cgaggtatgc gaccttcgcg
     3901 acaacgactg tgatggaagc accgacgaag gatttggccc ggtgacgtgc gggctcggct
     3961 cctgctccca caccgtcatg tcctgcgtca acggagtcaa acaggcgtgc gacccgttcc
     4021 agggcgccgt tgacgaactc tgcaacggcc tggacgacga ttgcgacggc aagagcgacg
     4081 acggtctctg gatgcccgaa aacctgcacg aaatcaactc cacctgcgcc gtcgcgaaag
     4141 atctgggcat catcaccgag ggcgagccat tgaagacgtt ccagtcatac atcgggaagg
     4201 ccggggacat cgactggttc aatttccgcg cggacgaaca cagccactcc tgcgccctgg
     4261 gcatggatca ggactacaaa atctacgcga ccctgttcaa tcccgccggg cccgaagtgt
     4321 gcatgccgat tgtcatggaa ctttacgacg acaagtgccg acttgtggca agcgcggttg
     4381 actccgactg ctcgtcccac aggatcgaat tcaagtggga cggcgaatgc ggtcccgacg
     4441 acagccgtga cttccgcatg aagatctggg cattcaacaa ggaacagtac agttgcatgc
     4501 cgtacaccct ccagctctac atgcgcaagg tcaactgacc cacccaccgg ggcgccgcta
     4561 ccacggcggc gccccgcctc cctttttcac catcgccccc atccgaaagc gcaccggatg
     4621 cattaatggc ctgcgcgacg tataatccgc ccggaacaac tcggggtgtc caatgccttc
     4681 ttgcccgtcg gaacacatca aaccggaaga cctgctggtt gaaacacttg gtgaatgccg
     4741 tttcccatct ccgcttggcg cgaagtattt cgtgaacgac gaagaccgca tttcgctgac
     4801 cccgtacatg cggaaactga ccgaccgcat tggacgaaac gagacaatgc cttcctttga
     4861 accggccgga cccagggagc ggatcttctt cgagccttcc gaagtcaccg cggggatcgt
     4921 gac
//
LOCUS       DAOPPA010000226         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18695_length_4920_cov_4.257554, whole genome shotgun sequence.
ACCESSION   DAOPPA010000226 DAOPPA010000000
VERSION     DAOPPA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18695_length_4920_cov_4.257554"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKH54_07795"
     CDS             <1..796
                     /locus_tag="PKH54_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99832.1"
                     /translation="DSALSSAARIHVEDRRQMERQDWEISRMRNDLKGAQTTLIRAEK
                     LASVGQLAAGVAHEIGNPVGIILGMSDVLRQGGSSPEEVQRFSDEIHKATMRVDQTIR
                     ELLTFARPVRDEGARADVRQVVESTVKLVQTHKSFRNVMVCIDIGEECPVAEIRPSQL
                     QQVLLNLFLNAAAAMNGEGRIEVSCRCEDRRLSIAVRDFGPGIPEKDLQRIFDPFFTT
                     KPPGEGTGLGLAMSSQIVEIYGGEIEAESRIGEGAVFTVKLWMAQT"
     gene            890..1204
                     /gene="rplU"
                     /locus_tag="PKH54_07800"
     CDS             890..1204
                     /gene="rplU"
                     /locus_tag="PKH54_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOC99833.1"
                     /translation="MYAVIMTGGKQYRVNEGDTIDVERLPVEVGSEVVIDRVLLVGAE
                     DKIRLGKPVVTGAKVTCKVVDNGRGPKVLAFKKRRRQNSKRIRGHRQEFTRLVISGIK
                     VG"
     gene            1208..1456
                     /locus_tag="PKH54_07805"
     CDS             1208..1456
                     /locus_tag="PKH54_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOC99834.1"
                     /translation="MGRKKGQSGRNGRDSNGQRRGVKLYGGELAVPGNIIVRQLGNKF
                     YPGVNVGQGRDYTLFATAGGRIRFRVGRNRQFIDVVPE"
     gene            1576..2580
                     /gene="obgE"
                     /locus_tag="PKH54_07810"
     CDS             1576..2580
                     /gene="obgE"
                     /locus_tag="PKH54_07810"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOC99835.1"
                     /translation="MRFIDEATIWVSGGKGGNGQVSFRREKFVPKGGPDGGDGGRGGD
                     VVLQGDEGLKTLLDFHFHQKFIAEDGAAGQGSNKTGRDGRDRILKVPVGTMVFDENGE
                     LLADIEAHDATFIVARGGRYGLGNARFALPWRQAPFFSTEGGPGDQRTVRLELKLLAD
                     VGLVGLPNAGKSSLINHLSRAQAKVADYPFTTIVPNLGVVVVADRSFVIADMPGLIEG
                     ASEGAGLGHQFLRHCERTSLLAHLVDVSSTEDPEADFRVVRNELEAHGTGLGAKRFIL
                     VASKVDVPGCEENALKLEALAEKLKVRFFAISAMSGEGVADLCRAMAEGVFQPEQETW
                     "
     gene            2675..2926
                     /gene="rpsT"
                     /locus_tag="PKH54_07815"
     CDS             2675..2926
                     /gene="rpsT"
                     /locus_tag="PKH54_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOC99836.1"
                     /translation="MANHKQALKRHRQSLKARSRNRHFKTMLRNVVKKALAAGTEEAG
                     QAFRTAESVVMHIASKGIIPKKRASRKVSRLAKRLQASA"
     gene            3129..3359
                     /locus_tag="PKH54_07820"
     CDS             3129..3359
                     /locus_tag="PKH54_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC99837.1"
                     /translation="MKELLEIIVKSIVDNEGAVKLSVVESESTIVIELSVAKEDMGKV
                     IGRDGSMAWALRTLVTNAAAKHRRRAVLQILD"
     gene            3517..4674
                     /locus_tag="PKH54_07825"
     CDS             3517..4674
                     /locus_tag="PKH54_07825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC99838.1"
                     /translation="MKRLIAFAALAVLVLAVSGCQKKQAAVETAAVEPAAKPDYVAVV
                     NGVKIPRSIFDEELAKVTQNGVRKIPEDRLLKISDNIVNRLIEEELLTQEVRKQGVVV
                     TPEETEAQYDEYKARFKGEEQFDNYLKHGKVTPEIIKERLTRSYSLTKLLDKLGKLSV
                     SDEDIQNAYNAGIRMFTDPEQVHAAHILVRLNEKDDAEKEKEAKAKIEGALKKLKKGE
                     DFGEIAKAVSEDTTTAPKGGDLGFFKAGQMVPAFEKAAFALKAGEYTREAVRTPFGLH
                     LIKSFEKKPERVRPLDEVREKVATSLRNKAMFKARRELVKSLRSTAEIEKLEGDVNAT
                     GADPAPAAVPAAPEAPAPAPQPAVEAAPQAPAPAPANAAAPAPAAEAPAGN"
     gene            4713..>4920
                     /locus_tag="PKH54_07830"
     CDS             4713..>4920
                     /locus_tag="PKH54_07830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOC99839.1"
                     /translation="MGARPGLELDEPVIFERGAAGRNGMCIPDAGLKGEPAIPAHLLR
                     TEPAALPEVAEYEVVRHFTRLSRMN"
BASE COUNT         1027 a         1438 c         1554 g          901 t
ORIGIN      
        1 cgactcggcg ctttcttcgg cggccaggat ccacgtcgag gatcgccggc agatggagcg
       61 ccaggactgg gaaatcagca ggatgcgaaa cgacctgaag ggcgcgcaga cgacgctgat
      121 ccgcgccgaa aagctcgctt cggtcggcca attggccgcc ggtgtcgcgc acgaaatcgg
      181 caacccggtc gggataatcc tgggcatgtc ggacgtgctt cggcagggcg gctcctctcc
      241 cgaggaggtt cagcgcttct ccgacgaaat tcacaaggcc accatgaggg ttgaccagac
      301 gatccgcgaa ctgctgacct tcgcgaggcc cgtccgggac gaaggggcgc gggccgacgt
      361 caggcaggtc gttgaatcga ccgtgaaact ggttcagacc cacaagtcct tcaggaacgt
      421 catggtgtgc atcgacatcg gggaggagtg cccggtggcc gaaataagac cttcacagct
      481 ccagcaggtc ctgctgaacc tgttccttaa cgcggcggcc gccatgaacg gggaagggcg
      541 catcgaagtc tcgtgccgat gcgaggatcg caggctttcg attgccgtca gggatttcgg
      601 gcccggcatc cccgagaagg atcttcagcg catcttcgac ccgttcttca ccacgaagcc
      661 ccccggggaa ggcaccggac tggggctggc gatgagttcc cagatcgtcg agatctatgg
      721 cggcgagatc gaggccgaga gccggatcgg cgagggggcc gtctttaccg tcaagctctg
      781 gatggcccag acctgaagga caatcccggg ttccctgatt ttgatgttga catttcgaca
      841 atcccgatta caatttccgt cccgttcgtt tggatgtttg aggtccaaca tgtatgcagt
      901 gataatgacc ggcggaaagc agtaccgcgt gaacgaaggc gacacgatcg atgtcgagcg
      961 cctgcccgtt gaagtcggca gcgaagtcgt gattgaccgt gtgctcctgg ttggcgccga
     1021 agacaagatc cgcctgggta agccggttgt taccggcgca aaggtgacct gcaaggtcgt
     1081 ggataacggt cgcggaccga aggttctcgc cttcaagaag cgtcgtcgcc agaattccaa
     1141 aagaatccgc ggccaccgtc aggaatttac ccggctggtc atctccggaa tcaaggtggg
     1201 gtgagccatg ggaagaaaga agggtcaaag cggccgtaac ggccgtgaca gcaacggaca
     1261 gcgccgcggc gtgaagcttt acggtggcga acttgccgtt ccgggcaaca tcatcgtccg
     1321 gcagctcggc aacaagttct atcccggcgt caatgtcggc cagggccgcg actacaccct
     1381 gttcgcgact gccggtggca ggattcgctt cagggtcgga cgcaaccgcc agttcatcga
     1441 tgtggttccg gaatagtccc cgggtcgcct gcccgctttg acgggccggt tcccgattca
     1501 aggcattttc cgcatcgatt catggcgttc acgccgcttc cttcaagcac cggcatcact
     1561 gttttttggt gacgtatgcg tttcatcgat gaagccacca tctgggtctc cggcggcaag
     1621 ggcggaaacg gccaggtcag tttccgtcgc gagaagttcg tcccgaaggg cggaccggac
     1681 ggcggcgacg ggggacgtgg cggggacgtc gtgctccagg gcgacgaagg cctgaagacg
     1741 ctgctggatt ttcattttca ccagaagttc atcgccgagg acggtgcggc cggccagggc
     1801 tcgaacaaga caggacgcga cggcagggac aggatcctca aggttcccgt cggaacgatg
     1861 gtctttgacg aaaacggtga gctgcttgca gacatcgagg cccatgatgc cactttcatc
     1921 gtcgccaggg gcggtcgcta cggcctgggg aatgccaggt tcgcccttcc ctggagacag
     1981 gctcccttct tttcgaccga aggcgggcct ggcgaccaga ggactgtcag gcttgagctg
     2041 aagctgcttg ccgacgtcgg tcttgtcggc ctgccgaacg ccggcaaatc cagccttatc
     2101 aaccaccttt cgcgcgccca ggcaaaggtc gccgattatc ccttcacgac catcgtcccg
     2161 aacctgggcg tggtcgtcgt cgcggacagg tcgttcgtca tcgccgacat gcccgggctg
     2221 atcgagggcg cttccgaagg ggccggactt ggtcaccagt tcctgcgcca ctgcgagcgc
     2281 acaagcctgc tggcgcacct tgtcgatgtg tcttcaaccg aagaccccga ggccgatttc
     2341 agggtcgtcc gcaacgagct tgaggcgcat ggaaccggcc ttggagcgaa acggttcatt
     2401 ctggtcgcct cgaaggtcga tgttccgggg tgcgaggaga acgcgcttaa gctcgaggct
     2461 cttgccgaaa agctgaaggt gaggttcttc gcgatatccg cgatgtcggg tgaaggtgtc
     2521 gccgatctgt gccgggccat ggcggaaggc gtgttccagc cagagcagga gacgtggtga
     2581 aggttcagac gggcgctgaa acctgttgac agacagagcc cgttcgagta taagactccg
     2641 gcgtttttgt ccgggctttt tttggaggtc cctcgtggcc aatcacaagc aggcgctcaa
     2701 gagacatcgg cagagcctca aggctcgttc ccgcaacagg catttcaaga cgatgcttcg
     2761 caacgtcgtc aagaaggctc tggcggccgg cactgaagaa gccgggcagg cattccgcac
     2821 cgcggagtcg gtcgtgatgc acatcgcttc caagggaatc attcccaaga agcgcgcatc
     2881 ccgcaaggtc tcaaggctcg cgaagaggct ccaggcctcc gcctgatccc cggacgacct
     2941 ccccttccgg cactgttccc cgggcttgcg gcccggccga cggcccgaac gattccgacc
     3001 gggttgcctg cacagggttt cgccatacgg ttttccccaa aaagtttctt tactttacac
     3061 attctgttaa catcgtcgcg attgtacact atgcagtgcg ggcatcacca ttccgtgcgg
     3121 agcgtcagat gaaagaactg cttgaaatta ttgttaaatc gatcgtagat aacgagggcg
     3181 ccgtcaagct gtccgtagtg gaaagcgaat cgacgattgt aatcgagctc agcgtcgcca
     3241 aggaggacat gggcaaggtt atcggcaggg acggttcgat ggcctgggcg cttcggacgc
     3301 tggttacaaa cgcggctgcc aagcatcgca ggcgggccgt gctgcagatc ctggactgag
     3361 ccgtccgcga cgcatcacag caatcacatc cgcgggaata tccggacatc caatcgcgat
     3421 tacaagctcg gtacgcgtcg tacgggcacg tttactttgc ggctttcgtg cgatagaatc
     3481 cgcacggctt ttttccagac gatcggagga cctgccatga agcgtttgat tgcgtttgct
     3541 gctcttgcgg tgctggtgct ggccgtttcg ggatgccaga agaaacaggc ggcggtcgag
     3601 accgcggccg tcgagccggc tgcaaagccg gattatgtcg cggtggtcaa cggggtgaag
     3661 attccccgtt cgatatttga cgaagagctt gcaaaggtta cccagaacgg ggttcgaaag
     3721 atccccgagg atcgcctgtt gaagatctcg gacaacatcg tgaaccggct gatcgaggaa
     3781 gaactgctta cccaggaggt tcgcaagcag ggagtcgtcg tgacgccgga agaaaccgag
     3841 gcccagtacg acgagtacaa ggcccggttc aagggcgaag agcagttcga caactacctg
     3901 aagcacggaa aggtcacgcc cgaaatcatc aaggaacgcc ttaccaggag ttactcgctg
     3961 acgaagctgc tggacaagct cggcaagctt tcggtctcgg acgaggacat ccagaacgcc
     4021 tacaacgccg gaatcaggat gttcaccgat ccggagcagg ttcatgccgc ccacatcctt
     4081 gtcaggctga acgaaaagga tgatgccgaa aaggaaaagg aagcaaaggc gaagatcgag
     4141 ggcgccctga agaaactgaa gaagggcgaa gacttcggcg agatcgcaaa ggcggtttcc
     4201 gaagacacca caacggcgcc gaagggtggc gatcttggat tcttcaaggc cggccagatg
     4261 gttccggctt tcgaaaaggc cgctttcgcc ctgaaggcgg gcgaatacac cagggaggcg
     4321 gtccgcaccc cgttcggtct tcacctgatc aagtcgttcg agaagaagcc cgagcgagtc
     4381 aggccgcttg acgaggttcg cgaaaaggtg gccaccagcc tgcgcaacaa ggccatgttc
     4441 aaggcccggc gcgagcttgt gaagtcgctt cgctcgaccg ccgaaatcga gaagcttgaa
     4501 ggcgacgtga atgcgaccgg ggccgacccg gcgccggccg cggttcccgc tgcccccgaa
     4561 gctcccgccc cggcccccca gcctgccgtc gaggccgccc cgcaggcgcc cgcaccagca
     4621 ccggcaaacg cggccgcgcc ggcccctgcc gcggaggctc ccgcaggcaa ctgaaatgta
     4681 cgttcactcc accaggaatt ttggaggaaa aaatgggtgc tcgtccagga ctcgaacttg
     4741 atgaaccggt catttttgaa aggggcgccg cgggaagaaa cggaatgtgc attccggacg
     4801 ccggactgaa gggggaaccg gccattccgg cgcatctgct gcgcaccgag ccggccgccc
     4861 tgcccgaggt cgccgaatac gaggtcgtcc ggcacttcac ccgcctgtcc cgcatgaact
//
LOCUS       DAOPPA010000227         4911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18741_length_4911_cov_4.695222, whole genome shotgun sequence.
ACCESSION   DAOPPA010000227 DAOPPA010000000
VERSION     DAOPPA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18741_length_4911_cov_4.695222"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKH54_07835"
     CDS             <1..327
                     /locus_tag="PKH54_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99840.1"
                     /translation="GRSFIGHLLAICDRLPRGASSAAWTAGDVRQQAEKAGTLVTRAD
                     RPVVGDIVFFSCGSGCGADAHEGTGAGVVIEESPDGPAFVAYHDGVVKVMKGMSVDQL
                     TGSCPP"
     gene            complement(300..977)
                     /gene="tmk"
                     /locus_tag="PKH54_07840"
     CDS             complement(300..977)
                     /gene="tmk"
                     /locus_tag="PKH54_07840"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOC99841.1"
                     /translation="MNVSRKGLFIVFEGLDGAGTTTQTRLLAARLAQRTPTVMTAATA
                     EPSTGPAGSVIRQVLRHRVRGTDCFGKDKPFDPGALALLFAADRLDHYSCEIEPVLDS
                     GGVAISDRYKLSSLAYQALDVPFEWIASINSLAPEPDLLFFLDVSPEVAWNRVSMNRI
                     TRDIFEVPEILRQVHANYNKALERMDRDKVVIIPGETPADSIADIVWHHVAGRIPAAQ
                     GGQEPVN"
     gene            complement(989..1798)
                     /locus_tag="PKH54_07845"
     CDS             complement(989..1798)
                     /locus_tag="PKH54_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOC99842.1"
                     /translation="MKPVQVKWLPMAAVCLFMFVPGCAGKLHPLPSGADWISDPSVLL
                     SRIVERKQRIRTVSTVGRIDSRSKAGLLRGRITTLVSRDRQVRLEAWTPSWDLAGSFV
                     GNADTFIYFQRGDKYCVAGTSDLETIDKVLPLGIDIDQYVNSLLGSCFIPENVSWKIE
                     FDRRCGCWLLSGSPGDGSTHRIRADADGTVRQSTVSAGSKTLIDIRFKGISTVSSDAG
                     SERFPAEITFDIPGRRSKATLKFKETEINGEVSDDDWEQTCPEGLGRMYLR"
     gene            complement(1795..2031)
                     /gene="rpoZ"
                     /locus_tag="PKH54_07850"
     CDS             complement(1795..2031)
                     /gene="rpoZ"
                     /locus_tag="PKH54_07850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOC99843.1"
                     /translation="MARVTVEDCLDTVTNRFALCLLAAKRARALLDDAEPLIETTNRE
                     TVTALREIAAGRIGFRRDVREMLDLPDRFGEKTR"
     gene            complement(2103..3101)
                     /locus_tag="PKH54_07855"
     CDS             complement(2103..3101)
                     /locus_tag="PKH54_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOC99844.1"
                     /translation="MILGLTGKNASGKGTVAEVLKTGGFEYFSLSDEIRRSLEQQGIP
                     ITRDTLTEEGNRIRRQEGAGALAARVIGRFSHGVNQVVDSIRNPQEVAVLRQQQNFFL
                     VAVDAPCETRFERLRQRARPGDPDTLEGFLAAEERELQSGDPVSQQLLATIELADYTI
                     NNSDGKDAVVAAVRNVFRDAAARTVRPTWDEYFMAIARVISSRGNCAKRRVAAVVVKD
                     SRIVSTGYNGTPRGTSNCNSGGCPRCLSLTPSGEALGECVCSHAEENAITQAAYHGVS
                     LKGATIYSTFMPCIMCTKMIINAGISEVVYHSDYPLPPEAASLFRQAGVSTRQFRG"
     gene            3232..3453
                     /locus_tag="PKH54_07860"
     CDS             3232..3453
                     /locus_tag="PKH54_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99845.1"
                     /translation="MGDNEQANIMDDESSRKSQVKIDTPFFCKTMQADMMVGRCIDLY
                     VEANAFGFKDSVCFKCMQGMKIRQLLAHD"
     gene            complement(3463..4362)
                     /gene="era"
                     /locus_tag="PKH54_07865"
     CDS             complement(3463..4362)
                     /gene="era"
                     /locus_tag="PKH54_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOC99846.1"
                     /translation="MKEGFKCGVVAIAGRPNVGKSTFLNKVTGSKLAIVTPKPQTTRD
                     RILGIFSGDDGQILFHDTPGIHESSKPMNRRMVARAISALADADVVLMMTDATRPEQA
                     IREDATVIARVRESGKPAVLAINKIDRIDKPLLLPLMSEYMKHGNFAAVIPISARKGG
                     GVNDVVKELLRMLPAGEPLFPEDELSDRPLRFLAAEIIREKVTLLTHQEVPYSTAVTI
                     ESWTDGSARKATRIEATIHVERDSQKAIVIGQGGSLLQRIGTQARVEIEELAGSRVML
                     SLNVVTEDNWTESDKNLDRLLGE"
     gene            complement(4359..>4911)
                     /locus_tag="PKH54_07870"
     CDS             complement(4359..>4911)
                     /locus_tag="PKH54_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HOC99847.1"
                     /translation="GRTPLIAADVGTGSGAIACTIATRNADVRLVASDVSQPAMDVAV
                     QNARTLEVADRIEFIRSDVLEDYPAGMSFDLIVSNPPYIAESEAGMVDASAIGFEPSH
                     ALFSGPEGTDMTYALIRQSLSRLQPWGALAVEIGTPAQASRVQERMRTAFESVTQIVD
                     PSGAVRGLAGRCPRRGDSTEHSK"
BASE COUNT          951 a         1435 c         1441 g         1084 t
ORIGIN      
        1 ggaagatcct tcatcggcca tctgcttgcg atttgcgaca ggcttccacg cggggcgtcg
       61 tcggctgcgt ggacggccgg cgatgtcagg cagcaggccg aaaaggcggg gacgcttgtc
      121 acccgggccg acaggccggt cgtcggcgat atagtgttct tttcctgtgg atccggttgc
      181 ggggccgatg cccatgaggg aaccggcgcc ggtgttgtaa tcgaggagag tccggatggc
      241 ccggcattcg tcgcatacca cgatggtgtg gtgaaggtga tgaaagggat gagtgtggat
      301 cagttgaccg gttcctgccc gccctgagcg gcgggaatcc ttccggcgac gtggtgccag
      361 actatgtccg caatagagtc ggccggtgtt tccccgggaa tgattacgac cttgtcccga
      421 tccatccgct cgagcgcctt gttgtagttt gcgtgaacct gtcgcaggat ttcgggaacc
      481 tcgaatatat ccctggtaat gcggttcatg ctgaccctgt tccaggcgac ttccggcgag
      541 acatccagga agaacagcag gtctggttca ggcgcgagcg agttgattga cgcaatccat
      601 tcaaaaggta catcgagtgc ctggtaggca agcgatgaaa gcttgtacct gtcggaaatc
      661 gcgactccgc cggaatccag gaccggctcg atttcgcacg agtaatgatc cagccgatcc
      721 gccgcgaaca gaagggcgag tgccccgggg tcgaacggct tgtcctttcc gaaacagtcg
      781 gttccgcgca cgcggtggcg aagcacctgc ctgatgactg aaccggccgg accggtactc
      841 ggttcggccg ttgccgccgt catcaccgtc ggtgtgcgct gcgccagcct ggcggcaaga
      901 agtctcgtct gcgtggtggt tccggcgccg tcaagccctt cgaagacaat gaagagacct
      961 ttgcgggaaa cgttcatcag gtcactcctt atcgcagata catccttccg agaccttcgg
     1021 gacaggtctg ttcccagtcg tcatcggaca cttctccgtt gatctcggtt tctttgaact
     1081 tcagcgtggc ctttgacctt cggcccggga tgtcgaacgt gatttcggcg gggaaccttt
     1141 ccgatcccgc atctgaagag accgtggaaa ttcctttgaa gcgtatgtca atcaaggtct
     1201 ttgaacctgc cgatacggtc gattggcgca ccgttccgtc agcgtcggcg cggatccggt
     1261 gagtcgagcc gtcgcccggc gagcctgaca gaagccagca tccgcatcgc ctgtcaaatt
     1321 cgatcttcca tgagacattc tcgggaatga agcaggaacc gagcagtgag ttgacgtact
     1381 ggtcgatatc gattccaagc ggcaggacct tgtcaatcgt ttccagatcc gatgtccccg
     1441 ccacgcagta tttatcgccg cgctggaagt agatgaatgt atcggcgttc ccaacaaacg
     1501 aaccggccag atcccagctc ggggtccaag cctcaagcct cacctgccga tcccgcgaca
     1561 ccagcgtcgt aatccgacct cgcagaagcc cggcctttga acgactgtca atgcgcccga
     1621 cggtcgaaac cgtccggatc cgttgctttc tttcgacaat tcttgacagc agcaccgaag
     1681 gatccgagat ccagtcggca cctgatggaa gcgggtggag tttcccggca caccccggga
     1741 cgaacatgaa caggcagacg gccgccatcg gcagccattt cacctgaacc ggtttcatct
     1801 ggtcttttcg ccgaaccgat ccggcagatc cagcatttcg cgtacgtcgc gcctgaagcc
     1861 gatcctgcca gcggcgattt cccgaagggc cgtgacggtt tccctgttag ttgtctcgat
     1921 cagaggctcc gcgtcgtcaa gcagcgcccg ggcacgcttg gctgccagaa ggcagagcgc
     1981 gaacctgttg gttacggtgt ccagacagtc ttcaacggta actcttgcca tgtaagcacc
     2041 tccggccggg aaatttagtt tatctgtggg gttttgtcaa tccgaaccgg ccgatcgacc
     2101 gcctagccgc ggaactgcct tgtcgatacg cccgcctgcc tgaagaggct ggcggcctcg
     2161 ggcggaagcg gataatcgga gtgataaaca acctccgaga ttcccgcgtt gatgatcatc
     2221 ttcgtgcaca tgatgcacgg catgaatgtc gaatatatcg tggccccttt caagctgact
     2281 ccatgatagg cggcctgggt gatggcgttt tcttccgcgt gtgaacacac gcattcgcca
     2341 agggcctcgc ccgacggagt aagggaaagg cagcgcgggc agccacccga attgcagttt
     2401 gatgttccac gcggagttcc gttgtaaccg gttgaaacga ttcggctgtc cttgaccaca
     2461 acagcggcga cgcgtcgctt ggcacagttc ccgcggctgc tgatgacccg ggctatggcc
     2521 atgaagtatt catcccatgt gggcctgacc gttcttgccg ccgcgtcgcg aaagacgttt
     2581 ctgacggcgg ccacaacggc atcctttcca tcggagttgt tgattgtgta atcggcaagt
     2641 tcaatcgtgg cgagcagttg ctgggaaacg ggatcgccgc tttgaagctc gcgctcctcg
     2701 gcggccagaa agccttcaag tgtgtcgggg tcgccaggtc gggcgcgctg tcgcaagcgc
     2761 tcaaagcggg tctcgcaggg tgcatcgacg gcaacgagaa agaagttctg ctgctgccgc
     2821 agaactgcga cttcctgggg attgcggatg gaatcgacaa cctggttgac tccgtgcgaa
     2881 aagcggccga ttaccctcgc cgccagtgcc ccagcccctt cctgcctgcg aatcctgtta
     2941 ccttcctcag taagcgtatc tctggtaatc ggaatgccct gctgttcgag cgatctgcgg
     3001 atttcatcgc tgaggctgaa atactcgaat ccacctgttt tcagcacttc ggccacggta
     3061 cccttgccgg aagcgttctt tcctgtcagg ccaagaatca tgataccccc tgaatatcca
     3121 gcactgttga acctgcccgc agcgccagtc ttgacggttt caaaaccgtt ctgaataatc
     3181 taccactatt ccgacttccg ggtgatatct ggattcgagg cggacatgcg aatgggcgac
     3241 aacgagcagg cgaacataat ggacgatgaa tcgagcagga agtcccaggt caagattgat
     3301 accccgttct tctgcaagac gatgcaggcc gacatgatgg tcggccgctg catcgatctg
     3361 tatgtggaag caaatgcgtt cggcttcaag gattcggtgt gcttcaagtg catgcagggc
     3421 atgaaaatcc ggcaactgct ggcgcacgat tgataagagg catcattctc cgagcagcct
     3481 gtcgaggttc ttgtcggact ctgtccagtt gtcttcggtg actacgttaa gggacagcat
     3541 cacccgactt ccggcgagtt cctcgatttc cacccgggcc tgcgttccga tcctctgcaa
     3601 aagggatccg ccctgtccga tcacgatggc cttctgggaa tccctttcga cgtgtatggt
     3661 cgcttcaatc ctggttgcct ttcgggcgct tccgtccgtc cacgattcga tggtcacggc
     3721 tgtcgaatat ggaacctcct gatgcgtcag cagggtcacc ttttcccgga tgatctcggc
     3781 ggcaaggaac ctcaggggcc tgtcggagag ttcgtcctcg ggaaaaagcg gctcgccagc
     3841 cggcagcatg cgcagcagtt ccttaacgac atcgttgacc ccaccgccct tccttgcgga
     3901 aatcgggatc accgcggcga agtttccatg cttcatgtat tcggacatca gcggcagaag
     3961 cagtggtttg tcgatgcggt caatcttgtt gattgccagg acggccggct tgccggattc
     4021 ccttacgcgg gcgatcacgg tggcgtcttc gcgaatggcc tgttcgggcc tggtggcgtc
     4081 ggtcatcatc aaaacgacgt ccgcatcggc aagggccgaa attgccctgg cgaccatcct
     4141 cctgttcatc ggcttcgatg attcgtggat gcccggggtg tcgtgaaaca ggatttgacc
     4201 gtcatcaccg gagaagatgc ccagaatcct gtcccgggtc gtctgcggct tgggcgtgac
     4261 gatggccagt ttgcttcctg tcaccttgtt gagaaaggtc gattttccga cattcggtct
     4321 acctgcaatc gcgacgacac cgcacttgaa tccttctttc actttgaatg ctccgtggag
     4381 tcgccccgcc gggggcatct tcctgcaagg ccgcgaaccg cccctgatgg atcgacgatt
     4441 tgcgtaaccg attcgaatgc cgtccgcatc ctttcctgaa cccttgatgc ctgggccggg
     4501 gttccgattt ccaccgcaag ggcaccccag ggttgaaggc gggacaggga ctgccggata
     4561 agggcataag tcatgtcggt tccttccggg cccgagaaaa gcgcatgaga cggttcgaag
     4621 ccgatggccg atgcatcgac cattccggct tcgctttcgg caatgtacgg tggattggag
     4681 acaatcaggt cgaatgacat cccggcaggg tagtcttcaa ggacgtcgga tctgatgaac
     4741 tcgatacgat ccgcgacttc cagcgtccgg gcgttttgca ccgcgacatc cattgccggc
     4801 tggcttacgt ccgaagcgac aagcctgacg tcggcatttc tggtcgcgat tgtgcaggcg
     4861 atcgcgccgc tgcccgttcc cacgtcggcc gcgatcaggg gcgtccttcc t
//
LOCUS       DAOPPA010000228         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18773_length_4904_cov_3.495979, whole genome shotgun sequence.
ACCESSION   DAOPPA010000228 DAOPPA010000000
VERSION     DAOPPA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18773_length_4904_cov_3.495979"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..937
                     /locus_tag="PKH54_07875"
     CDS             59..937
                     /locus_tag="PKH54_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOC99848.1"
                     /translation="MKSIYVESLASYNNKTTLIRNNNCDYCNLIGLRLADNQSLKGAS
                     LIQANLTNARIVGADLSGAVADGAIFRGANMSSCNLKGAKLSNTVFEGDDHTQPADLS
                     YAYMPNARLDHAHMNGVNAAYAQIYGGQATVAQATMQGIQLPNSVLCNMDFSQATMKG
                     ASLTGANLISTTFRGVDLTGANLVGALLQGTVFDGATLYGAKLQNAGIAFEGGSLQVT
                     RLGDDNTLQVVTVSFDKTSLPPDTTNGDTFCPYGGQSFEGLPGCDTTAELTSMDPPKP
                     PTCIPSATQFCDRTRK"
     gene            complement(944..2998)
                     /locus_tag="PKH54_07880"
     CDS             complement(944..2998)
                     /locus_tag="PKH54_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99849.1"
                     /translation="MIGIRNRMIVLASFCGTLIWAGCGGGIPVNDSIEEIQDSVAESD
                     APPDSLIPPDSEDDARPDSIQVPDAVPDSADASDIAQEDLQTDPGDDAVDFEIDAGSD
                     VGDVDDSGLADDGSDTDADDVKAGDLSDGGDGDAIADQDVTDDSVMYDVQSDSADPGF
                     SPTICRPCRVDSDCAAEGYQPVASCIPVSDPSGLAGSFCLIPCLEDSGCPDGYECLDS
                     DDSGNKACYWAGGDGQPCECGVLQSGADTSCTRANIHGTCEGVAECTLTGFAPCGAAI
                     PAAEICDDVDNDCDGLTDEFLGSSPCGLGECQHDVLNCVDGNPGNCEPMQGAVVETCD
                     GKDNDCDGMTDQGFLFTEVDGSQLAKGADCGVGACAGGTVVCSPDQLGLTCDSLYKSS
                     IEILNDIDDDCDGLIDEGLCRPDCTGRECGGDGCGGSCGTCALGYSCGSSGICVPDGM
                     VQVPFSMFYMGCDPATDPACRPGENPRHYVTISGFFIDRTEVTVAAYALCVGAGACSA
                     TRTEGLCQWGHSGREQYPINCVTWEQAQSYCSWVGKRLPTEAEWELAARGTDERLYPW
                     GNGAPSCGLAVMLESTTPGCGTGVPAIPCSRSPAGDSPFGACDMSGNVQEWVFDWYDP
                     NYYSSSPPLNPHGPDAGSTRVTRGGHYAVHDAAWLRSAARGEAAASIDGVETGFRCAK
                     TH"
     gene            complement(3131..3979)
                     /locus_tag="PKH54_07885"
     CDS             complement(3131..3979)
                     /locus_tag="PKH54_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC99850.1"
                     /translation="MKSVAQFWNRPASVVLILLCACGGEAAPSGEIGVLTYNVAGLPA
                     ELSGSDPVANMPRIAPLLNAYDLVLVQEDFWYHADLDAGTTHPHKSPPMWETPDWDKM
                     GDGLNTFSAYPSGEFTRVAWEQCNGLIDSGGDCMTTKGFLYARLEVEPGIAIDVYNLH
                     MDASGSEEDIAARRAQSAQLASFIALNSEGVAVLLAGDTNLKATRPEDLQTLDDFLAA
                     TGLVDSCRFLECGDERIDRIMFRSCPDLELEPVEWSIPEEFVDADGNQLSDHLPVLAR
                     VAWRVL"
     gene            complement(3990..>4564)
                     /locus_tag="PKH54_07890"
     CDS             complement(3990..>4564)
                     /locus_tag="PKH54_07890"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOC99851.1"
                     /translation="TENSVMELVGSRLRTLSWSKDSTGAEQESWKLTYDWAGGKVHYN
                     YQDRAGGKKESKSVELGSTAFSSDAMYFMLRGFPFEQGPGARIDGDFVLTNGQVLPAY
                     IILRGEEKVTTPMGTFDTYKLEMKLKGALGPIAPKMFMWFTKDKPHLFVRYDGKEAGI
                     TSGKTVNVLLKFSPAEWIKPLPPPAPVATP"
     assembly_gap    4565..4574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4575..4784)
                     /locus_tag="PKH54_07895"
     CDS             complement(<4575..4784)
                     /locus_tag="PKH54_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99852.1"
                     /translation="MNRIACLVSVLAVALPAAALAAELGPIEIAPQESGVQMTRFEKK
                     TEALYNYSWNVKRDGDKVVFSAKGDN"
BASE COUNT         1023 a         1569 c         1378 g          924 t
ORIGIN      
        1 cttgttgttg taagacgcaa ggctctcgac cgacacgctt tccacctttt cggcaacggt
       61 gaagtcgatc tatgtcgaga gccttgcgtc ttacaacaac aagacgacac tgatcagaaa
      121 caacaactgc gattactgca acctgatcgg ccttcggctg gcagacaacc agagcctgaa
      181 aggcgcgtcc ctgatacagg ccaacctgac gaatgccagg attgtcggcg ctgacctgtc
      241 aggcgcggtg gccgacggcg ccatattccg cggggctaac atgtcctcgt gcaacctgaa
      301 gggagcaaag ctgtcgaaca ctgtattcga gggcgacgac cacactcagc cggccgacct
      361 gagttatgcg tacatgccga acgcccgcct ggatcacgcc catatgaacg gagtcaacgc
      421 cgcctatgcc cagatttacg gcggccaggc gaccgttgcc caggcaacca tgcagggcat
      481 ccagcttcca aattcagtcc tgtgcaacat ggacttcagc caggcaacca tgaaaggtgc
      541 ctcgcttacg ggggcgaacc tgatctcgac gacgttccgc ggggtcgatc tgacgggggc
      601 caaccttgtc ggcgcgctgc tccagggaac cgtgttcgac ggcgccacgc tttacggcgc
      661 caaactgcag aacgcgggaa ttgcctttga aggcgggtca cttcaggtta cgcgactggg
      721 ggacgacaac acccttcagg tggtgactgt cagcttcgac aagacttcac ttccgccgga
      781 cacgaccaat ggcgacacgt tctgtcccta tggcggacaa agcttcgaag gtcttcccgg
      841 atgcgacacg actgcggaac ttacatcgat ggatccgcca aagcccccga cctgcatacc
      901 gagcgccact caattctgcg accgcacaag aaagtaggca ggttcaatgt gtcttcgcgc
      961 agcggaagcc ggtctcgact ccgtcgatgc tggccgcggc ctcgccacgt gccgcgcttc
     1021 gcagccacgc ggcgtcgtga acggcataat gtccgcccct ggtaacccgt gtcgagcctg
     1081 catcaggccc gtgtggattc aaaggcggac tgctggagta ataatttggg tcataccagt
     1141 caaaaaccca ttcctgcacg ttacctgaca tgtcacaggc gccgaatgga ctgtcacccg
     1201 ccggcgacct ggaacagggg attgccggaa ccccggttcc gcaaccgggg gtggtcgatt
     1261 caagcatgac ggcgagcccg catgaggggg cgccgtttcc ccagggataa agacgttcat
     1321 cggttcccct ggccgccagt tcccattcgg cctcggttgg aagccgcttg ccgacccacg
     1381 aacagtatga ctgcgcctgt tcccaggtaa cgcaattgat cgggtactgc tcccgtccag
     1441 aatggcccca ctggcacagg ccctcggttc tggtcgcgga acacgcgccg gcgccgacgc
     1501 acagggcata cgccgcgacc gtgacctcgg tccggtcgat gaagaatccc gatatggtca
     1561 cgtaatggcg cgggttttcg ccgggacggc aggccgggtc ggtcgcggga tcgcacccca
     1621 tgtagaacat gctgaacgga acctgcacca tgccgtccgg gacgcagatg ccggaagaac
     1681 cacacgaata cccaagggcg caggttccgc aggatccgcc gcagccgtca ccgccacact
     1741 cgcgcccggt gcagtccgga cgacacagcc cttcgtcgat caagccgtcg cagtcgtcat
     1801 cgatgtcgtt aagaatctcg atggacgact tgtaaaggga atcgcaggtc aatccaagct
     1861 gatctggtga acagacgacg gtgccccccg cgcacgcgcc gactccgcag tctgcgccct
     1921 tggcaagctg gctaccgtca acttccgtga acaggaatcc ctgatcggtc atgccgtcgc
     1981 aatcgttgtc cttcccgtcg cacgtctcga caacggcccc ctgcatcggt tcgcagttgc
     2041 ccggatttcc atccacgcaa ttcaatacgt cgtgctgaca ttcccccagc ccgcatggcg
     2101 agcttcccag gaactcatcg gtcaaaccgt cgcaatcgtt atccacatcg tcgcaaatct
     2161 cggccgccgg tattgcggcg ccgcacggag caaatccagt cagggtacac tccgcaaccc
     2221 cttcacacgt cccatggatg ttggccctgg tgcacgacgt gtccgccccg gactgcagga
     2281 cgccgcactc gcacggctgt ccgtcgccac cggcccagta acatgccttg tttccagaat
     2341 catcggagtc aagacattca taaccgtccg ggcacccgga gtcttccagg catggaatca
     2401 ggcagaatga ccccgccagg ccggacggat cggaaaccgg gatgcatgaa gcgacgggct
     2461 ggtatccttc ggcggcgcag tcggaatcaa cgcggcacgg cctgcagatg gtggggctga
     2521 agcccggatc ggctgaatcg gattggacat catacataac ggaatcgtcc gtcacgtcct
     2581 gatccgcaat tgcgtcacca tcaccgccat cactcaaatc cccggccttc acgtcgtctg
     2641 catcggtgtc ggaaccatca tcggccagcc cggaatcatc gacgtcacca acatcactgc
     2701 cggcatcgat ctcgaagtcc accgcatcgt cgccaggatc tgtctgcaga tcctcctgcg
     2761 caatatcaga cgcgtcggca gaatcaggaa cggcatcagg gacttgaatc gaatccggcc
     2821 tggcgtcgtc ctcggagtcc ggcggaataa ggctgtccgg gggcgcgtcc gattcagcga
     2881 cggaatcctg gatttcctcg atcgaatcgt tgactgggat cccgccgccg cagccggccc
     2941 agatcagagt cccgcaaaac gacgcaagga caatcatccg attgcgaata ccgatcatgg
     3001 agctctccaa tgcagtagtc catctgggga ctatactccg gacgccggga ctgtcaaaat
     3061 cagtccgatg agagtctcat cggccaataa cctcgcaatt ccgttgtccc tggcaatggc
     3121 aggctcgctt ctacaagacg cgccaggcaa ccctggcaag caccggcagg tgatcggaaa
     3181 gctggttacc gtctgcatca acgaattctt cgggaatgga ccactcaacc ggttcaagtt
     3241 ccagatcggg acaactgcgg aacattattc gatcgattct ctcgtcgccg cattcaagga
     3301 accggcacga atcgacaagg ccggtggcgg ccaggaaatc gtcaagtgtc tgaaggtcct
     3361 cgggacgggt ggccttcagg ttggtgtcgc cggccagcag gaccgccaca ccttccgaat
     3421 tcagggcaat gaacgacgca agctgcgctg actgggccct gcgggcggcg atgtcctctt
     3481 ccgagccgct ggcgtccatg tgcaggttgt aaacgtcgat ggcgatgccg ggttcgacct
     3541 cgagacgcgc gtaaaggaat ccctttgtcg tcatgcagtc gccgccgctg tcgataaggc
     3601 cgttgcactg ctcccaggcg acgcgggtga actcgcccga cgggtatgcc gaaaacgtgt
     3661 tcaggccgtc acccatcttg tcccagtccg gggtctccca catcggaggt gacttgtggg
     3721 ggtgcgtcgt tccggcgtcc agatccgcgt gataccagaa gtcttcctgg acaagaacca
     3781 ggtcatacgc gttcagcagg ggcgcaatcc tcggcatgtt cgcaacggga tccgaaccgg
     3841 aaagctcggc gggaagtccc gccacattgt acgtcaggac gccgatttca ccggatggag
     3901 ccgcctcgcc gccgcaggcg cacaacagga tcaggacaac cgatgcaggc cggttccaga
     3961 attgcgcaac tgacttcatc aagtcacctt cagggagtcg caaccggggc cgggggcgga
     4021 aggggcttga tccactcggc cggactgaac ttcaacagga cattcacggt ctttccggac
     4081 gtgatgccgg cttccttgcc gtcataccgc acgaaaaggt ggggcttgtc cttcgtgaac
     4141 cacatgaaca tcttcggggc gattgggccc agggcgccct tcagcttcat ctccagctta
     4201 tatgtgtcga acgtgcccat cggcgtggtc accttttctt caccgcgcag gatgatgtac
     4261 gcgggaagaa cctgcccgtt cgtcagcaca aaatccccgt caatcctggc gccggggccc
     4321 tgttcgaacg gaaatccgcg cagcatgaaa tacatggcgt ccgacgagaa tgccgttgaa
     4381 cccagttcga cagacttgct ttccttcttg ccgccggctc gatcctggta gttgtagtga
     4441 acctttccac ccgcccagtc gtacgtcagc ttccagcttt cctgctcggc gccggtggaa
     4501 tccttggacc aggacaatgt ccgcagtctg cttccaacaa gttccatcac cgaattttcg
     4561 gtccnnnnnn nnnngttgtc gccctttgcg gaaaagacca ccttgtcccc atcacgtttg
     4621 acgttccagg aatagttgta cagggcctcc gtcttctttt cgaagcgggt catctgcaca
     4681 ccgctttcct gcggggcaat ttcaatgggg ccaagctcgg ccgcaagtgc cgccgccggc
     4741 agcgcgaccg caagaactga caccagacat gcaatcctgt tcacatttcc acctgcaacg
     4801 acgaatccac gtccgcgacg gggtccgcct gattttcggg tacatcatcc atggaaatcg
     4861 ccaggccaag ccgcggcgcg accacggaca cccacgaatc gaca
//
LOCUS       DAOPPA010000229         4901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18795_length_4901_cov_3.594305, whole genome shotgun sequence.
ACCESSION   DAOPPA010000229 DAOPPA010000000
VERSION     DAOPPA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18795_length_4901_cov_3.594305"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKH54_07900"
     CDS             complement(<1..689)
                     /locus_tag="PKH54_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOC99853.1"
                     /translation="MAQSFLFTSESVSEGHPDKVADQISDAVLDAALARDPESRVACE
                     TLVTTNLVVVAGEITCGGSIDYEGIARQTVARIGYDDPALGFDSKGARVQVHCHQQSA
                     DISRCVTSATDSLGDQGAGDQGMMFGYACDETPAFMPLPLHLAHRLVERQAAVRRSGD
                     LSGLRPDAKSQVTIEYVDGVPRRIDTVVLSTQHGPEYSNNQAALQEGIRELVIRPVLE
                     LEGFGLPANVYV"
     gene            919..1257
                     /locus_tag="PKH54_07905"
     CDS             919..1257
                     /locus_tag="PKH54_07905"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC99854.1"
                     /translation="MKDQDRQWMSVALAGVLSNRMRMSIVSHLQNGPCTVLKLCEAVG
                     LSQAVVSKQLGILRDSGLLICRPQGRVREYALQDPYGIARLLEAMDGAAQVASKNALN
                     CRKMDESSSN"
     gene            1269..1613
                     /locus_tag="PKH54_07910"
     CDS             1269..1613
                     /locus_tag="PKH54_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC99855.1"
                     /translation="MLDTNLKHIDSVEKFNDLVKSGRKAAIVCGRMGPMCIPVYGVME
                     SLEAKYPDVEFFDMEFDSPIGMKTIRSLPETRSFNGLPFTVYLRDGKVVAATSSIQSK
                     AQVKAILDEKLV"
     gene            1623..2558
                     /locus_tag="PKH54_07915"
     CDS             1623..2558
                     /locus_tag="PKH54_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC99856.1"
                     /translation="MNERFDAIVMGGGPAGLTAAIYLARARLKTLVVDNATIGGQTNL
                     THEVANYPGVLNVSGAGLARIMKSQATSFGARIIGHADIIDFDLSGPEKRIEIDDEGV
                     FTAPVVVLATGGVPRTLGLESESRFKGRGISYCATCDGDFFTGKEIVVIGGGNSALEE
                     AVSLTKYATKVTIVHEFDHFQAHPWAVEEARKNPAISFMMNQRVDGFAGGEKLEGVIT
                     TDKTTGATVTIPAEGAFLFIGYTPNTAWLKGQVTLNERGEIPVDESMATNIPGVFAAG
                     DARVKRYRQITTAVADGTIAALAAADYLARNQIQA"
     gene            complement(2671..3267)
                     /locus_tag="PKH54_07920"
     CDS             complement(2671..3267)
                     /locus_tag="PKH54_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOC99857.1"
                     /translation="MEKKEREEMVMVVPRDQVPDFQGFMAVGRDYVLALSSLGRYMKR
                     SLAEEDPSFKQLIPYAIIMSGGQVFKYRRGVKGGESRLHGLISVGVGGHISAVDEDLF
                     GEAYDKAFERELSEEVAMAPVLSNAVLGLINDDSNPVGRVHLGVVHLITLEIPEVKSR
                     EKALLDAGFVPLDELRSRYAELETWSQICLDNWNRIHD"
     gene            complement(3358..4089)
                     /locus_tag="PKH54_07925"
     CDS             complement(3358..4089)
                     /locus_tag="PKH54_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC99858.1"
                     /translation="MNGCGRILIETSAVEFSPEYCRLSPGYMPGRFLMISVSDTGCGI
                     DGETMERMVEPFFTTKKVGKGTGLGLSTVHGIVRQSGGFIAVSSEPGCGATFRVYLPR
                     CESPADELGFGISLGTGTANGQRILLVEDEPLIRDLARMMLESLGYHVSVAENPIEAL
                     KIVEEARGRFDLMLSDIVMPEMNGLELALNVRRINPDVRMLFMSGYTSDHVQIQEILN
                     ADAAFLPKPFSMEDLRLTIQRVLSS"
     gene            complement(4086..>4901)
                     /locus_tag="PKH54_07930"
     CDS             complement(4086..>4901)
                     /locus_tag="PKH54_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOC99859.1"
                     /translation="RESEEQLAALADNLAGGMVYQIDSGPDGTQRRLTYVSRGVETLH
                     ELTREDVGDNPLFIYQSIRPDYRQMVVDREEEAIRLMKRFYLEAPVILPSGRQAWRSF
                     SAAPRRLSSGHVVWDAIELDVTDRRKAQDEQASLEAQLAHSRKMESIGQLAGGIAHDF
                     NNMLAVILGHAEMALGRLALERVDPESPVCRDLASIRTAAERSADLTRQLLAFARKQA
                     VVPKVLDLNEAVSGTISTLGRLIGDAIELRWVPGIRARSHRSWSTSASTRVMR"
BASE COUNT         1087 a         1471 c         1374 g          969 t
ORIGIN      
        1 acgtaaacgt tggccggaag tccgaatcct tccagttcca gaacgggacg aatcacaagc
       61 tctcggatgc cttcctgcaa ggccgcctgg ttgttcgagt attccgggcc atgctgggtc
      121 gaaagaacca ccgtgtcaat ccttctcgga acgccatcga cgtattcaat cgtgacctgg
      181 gacttcgcgt ccggccgcag gcctgaaaga tcccccgatc ttctgacggc cgcctgtcgt
      241 tcgaccaggc gatgcgccag atgcagggga agcggcatga acgccggtgt ttcgtcacag
      301 gcatacccga acatcatgcc ctggtcgccg gcgccctggt cgcccaggga atcggtggcg
      361 cttgtaacgc aacggctgat gtcagcgctc tgctggtggc agtgaacctg aacccgggct
      421 ccctttgaat cgaaccccag cgcgggatcg tcgtatccga tcctggcaac ggtctgtctg
      481 gcgattcctt cataatcaat cgaaccgcca caggtgattt cgcccgccac gacgacaaga
      541 ttggtcgtga caagcgtctc gcaggcaacc cgtgactcgg gatcgcgggc cagggcggcg
      601 tccaggactg catcggaaat ctggtcggca accttgtcgg gatgaccttc ggataccgat
      661 tcggaagtaa aaaggaatga ttgggccatt ttttgctcct gtgtccccgt atgtttcgtt
      721 cgcgcgaccg gctcggggac tcacgagcgg cgacgcttta gcagcatttc tatcccgccc
      781 gcaagttgtt gcattaaaac ggcggctggg ccgatgttac tgcacaccgg gacaaaaatg
      841 caaacaccac ggcgacctcc tcttgacaca tatcaaaaac gatacatatt caatcccggc
      901 tgacgaatgg agccggaaat gaaggaccag gacaggcagt ggatgtcggt tgcccttgca
      961 ggcgtgcttt cgaaccgcat gcggatgtcg attgtctcgc acctgcaaaa cggcccgtgc
     1021 accgtcctga agctttgcga ggcagtcggg ctttcccagg ccgtcgtttc aaagcagctt
     1081 ggcatccttc gcgattccgg tcttctgatc tgccggccgc agggccgcgt gcgcgaatac
     1141 gcactgcagg atccgtatgg aattgcgcgg ctgctggaag ccatggacgg ggcggcccag
     1201 gttgcgtcga agaacgccct gaactgccgg aagatggacg aatcgtccag taattgaagg
     1261 aggacacgat gcttgatacc aacctgaaac acattgattc cgtggagaag ttcaacgatc
     1321 tggtgaaaag cggcaggaag gccgccatcg tatgcggcag gatgggcccg atgtgtatcc
     1381 cggtctatgg ggtgatggaa tcacttgaag caaaatatcc ggacgtcgaa tttttcgaca
     1441 tggaattcga cagcccgatc gggatgaaga cgatacgcag ccttccggaa accaggtcgt
     1501 tcaacgggct gcccttcacc gtgtatcttc gcgacgggaa ggtcgtggcc gccacatcca
     1561 gcatccagtc gaaggcccag gtaaaggcaa tccttgacga gaaactggtt tagggtggca
     1621 tgatgaacga acgttttgac gcgatcgtga tgggtggcgg gcccgctgga ttgacggcgg
     1681 cgatttacct ggccagggcc aggctgaaga ccctggtggt tgacaacgcg accatcggcg
     1741 gccagacgaa cctgacccat gaggtggcca actatcctgg cgtcctgaac gtttccggtg
     1801 ccggactggc ccgaatcatg aaaagccagg ccacatcatt cggcgccagg atcatcggcc
     1861 acgccgacat tatcgacttt gacctttccg gcccggaaaa gcgaatcgaa atcgatgacg
     1921 aaggcgtgtt caccgcgccg gtggtcgtcc tggcaaccgg cggcgtgccc aggactcttg
     1981 gccttgaatc cgagtcccgc ttcaagggcc gcggaatttc atactgcgcg acgtgcgacg
     2041 gggacttctt caccggaaag gaaatcgtgg tcatcggcgg gggcaactcc gcactggaag
     2101 aggcggtttc cctgacgaaa tacgccacaa aggtaaccat agttcatgaa ttcgaccact
     2161 tccaggccca tccctgggcg gtcgaggaag cgcggaaaaa ccccgcgata tctttcatga
     2221 tgaatcaacg tgtcgatggc tttgccggtg gtgaaaaact ggaaggagtc atcacgacgg
     2281 acaagacaac cggcgcgacc gtgacgatcc ctgccgaagg tgcgttcctg ttcatcggat
     2341 acacaccgaa cacggcctgg ctgaaaggac aggtcacatt aaacgaacgc ggggaaatcc
     2401 cggtggacga atccatggcg acaaacattc ccggcgtttt cgcggccggc gacgcccgcg
     2461 tcaagaggta ccgccagatt acgacggcag ttgcggacgg cacgattgcc gctcttgcgg
     2521 ccgccgacta cctcgcacgc aaccagatac aggcatgacg gattaaggca gcgtcgggac
     2581 tcgcttaccc caactctgat tccggctcgc acaacaccct gggattaccg cgatttcctg
     2641 tgtgccaaac gattcgatcg gcaggggaat tcagtcgtga atccggttcc agttgtccag
     2701 gcaaatctgg gaccaggtct caagttcggc atagcgtgaa cgcaactcgt ccaacggcac
     2761 gaatcccgcg tcgagcaggg ccttttcgcg tgatttcact tccgggatct ccagggtgat
     2821 aagatgtacc acgcccaggt gaacacgtcc gacgggattc gaatcgtcat tgatcaagcc
     2881 aaggacagca ttcgacagga ctggggccat cgcgacttcc tcggaaagct cccgctcaaa
     2941 cgccttgtca taggcttcac cgaagagatc ttcatcgacc gccgagatgt ggccgccgac
     3001 accgacggaa atcagcccgt gcagacgcga ttcaccaccc ttgacgccgc gcctgtactt
     3061 gaagacctgg ccgcccgaca tgatgattgc gtacgggata agctgcttga aagacgggtc
     3121 ctcttcggcc agcgaccgtt tcatgtacct tccaagcgat gacagcgcca gcacataatc
     3181 ccggccgacc gccatgaacc cctgaaaatc gggaacctgg tcccgaggta ccaccatcac
     3241 catctcttcc ctttccttct tttccatgat tagtccccga aacagttaca agacaactta
     3301 taccagcttt gacgggcgtg ggggcccgat aatcacgccc accccgggga caaggcatca
     3361 ggatgaaagt acccgctgaa tcgtcagcct cagatcctcc atggaaaagg gcttcggcag
     3421 aaaggccgcg tcagcattga gtatttcctg gatctggaca tgatcggacg tgtatccgga
     3481 cataaacagc atcctgacgt ccggattgat ccgcctgaca tttaacgcaa gctccaggcc
     3541 gttcatctcg ggcatgacaa tgtctgaaag catcagatcg aatctgcctc gggcttcttc
     3601 aacaatcttc aatgcctcga tgggattctc ggcaacgctg acatggtatc caagcgactc
     3661 cagcatcatc cgcgcgagat cccgtataag tggctcgtcc tcgaccagca ggatcctctg
     3721 gccatttgcg gtaccggttc ccagggaaat cccgaaaccc agttcatctg ccgggctctc
     3781 gcaacgtgga agatacaccc ggaaggtcgc cccgcatccc ggttcgctgg acacggcaat
     3841 aaacccgccg ctctgcctga cgattccatg aaccgtcgaa agccccagac cggtaccctt
     3901 gccgaccttc ttcgtggtga agaaaggttc gaccatcctt tccattgtct cgccgtcgat
     3961 tccacacccc gtatcgctga ccgaaatcat caggaatctg ccgggcatgt agccgggact
     4021 caggcgacag tattccgggg aaaactcgac tgcagaagtc tcgattagga tcctgccgca
     4081 gccattcatc gcatcacgcg agttgacgca gaggttgacc aggatctgtg cgacctggcc
     4141 cggatccctg gaacccaccg aagttcgatc gcatcgccga tcaacctgcc cagggtcgaa
     4201 atcgtgcctg aaacagcctc gttcagatca agaacctttg gaacaacggc ctgctttcgt
     4261 gcgaacgcca gaagttgtcg tgtaagatcg gccgaccgct cggcagccgt tctgatgctg
     4321 gcaagatccc tgcacacggg gctttcggga tcgacgcgtt caagcgcgag cctgcccagc
     4381 gccatctcgg catgaccaag gatgaccgcg agcatattgt tgaaatcatg ggcaataccg
     4441 cccgcgagct gaccgatcga ctccatcttc ctggaatggg caagctgggc ctccaggctg
     4501 gcctgttcgt cctgggcctt cctgcgatca gttacatcca gttcgatggc atcccatacg
     4561 acatgaccgg aagacaatcg cctgggtgct gcagaaaagc tgcgccaggc ctgacgaccg
     4621 gaaggaagga tcaccggcgc ctccagatag aagcgcttca tcaggcgaat cgcttcctct
     4681 tcccgatcaa ccaccatctg cctgtagtcg gggcgaatgg actggtaaat gaaaagcgga
     4741 ttgtccccga catcctccct ggtcagttca tgcagggttt cgaccccgcg actcacgtac
     4801 gtcagcctgc gctgggttcc gtcgggaccg gaatcaatct gatagaccat gccgccggcc
     4861 aggttgtccg ccagggccgc cagttgttct tcggactcgc g
//
LOCUS       DAOPPA010000230         4893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18838_length_4893_cov_3.658950, whole genome shotgun sequence.
ACCESSION   DAOPPA010000230 DAOPPA010000000
VERSION     DAOPPA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18838_length_4893_cov_3.658950"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKH54_07935"
     CDS             complement(<1..375)
                     /locus_tag="PKH54_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-5-alpha-steroid 4-dehydrogenase"
                     /protein_id="HOC99860.1"
                     /translation="MDFSSILAGEKVFHFWLMVVFLGMAALVFPLLLFVTAPYGRHTR
                     KGWGPRINDTVGWVIMEAPAPILFALFFFVGERTSSILPIVFLCMYELHYINRTFIFP
                     FRLRGNRNDMPVSIMMSGFLFNL"
     gene            complement(388..603)
                     /locus_tag="PKH54_07940"
     CDS             complement(388..603)
                     /locus_tag="PKH54_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99861.1"
                     /translation="MWLARAVAPAALLVSRITGLPALFTPESLNALRANSDIRHDKAA
                     RELGFNPRPLDESLKDIHDWFKEVGMI"
     gene            complement(715..1377)
                     /locus_tag="PKH54_07945"
     CDS             complement(715..1377)
                     /locus_tag="PKH54_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC99862.1"
                     /translation="MLTVVTGATGHIGGNLVRLLLAEGRKVRCLVWEHDDNTAALDGL
                     DVERVCGDVRDLESLKRAFSGADVVYHLASRISIVGPEGGLVRAINVGGAANVAEACI
                     QCGVRRLVHFSSIHARNINTDLDKPVDETRERSGGRGTPAYDISKGDGELEILKAVEK
                     GLDAVIVCPTGVIGPLDWKKSRMGTVFLGLATGKLPGGVQGGFDWVDARDVAAAAIAA
                     ES"
     gene            complement(1562..3796)
                     /locus_tag="PKH54_07950"
     CDS             complement(1562..3796)
                     /locus_tag="PKH54_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC99863.1"
                     /translation="MTISQIRDAILYVQNESIPEGDVVLSTSLPAREARLAPHPPGLH
                     PDVVDYLSRSGIESLYSHQAHACSVSMAGRNVILSTATASGKTLAFTLPVLHELATDP
                     DACALFLYPLKALTNDQLDVFSAVQDASGVQIDAAIYDGDTPRASRSRIRQLSRVILS
                     NPWEIHETLPYHHLWRRFFLNLRYVVIDEAHRYTGVFGSNVAQVIRRLVRVASVYGAS
                     PRFIMASASIANAGPHALALTGQDAVCIDDDGSPSGEKALIVVNGGLEGGGSPAARTR
                     DIVAGLVRRGIKTLCFTRSRRTAELVASLAADRFGGLPISPYRAGYLPAQRRQLEQAF
                     REGSIRGVVSTNALELGIDIGDLDAVVVSGYPGSISAFWQQAGRTGRRGGASAAIFVA
                     GGDILDQYLASHTEILTDRAFERATIDLDNPHILAGHALCAASELPLKVDEADSHLFG
                     IAKSLAGAGLLRETVNGFIYGGSVRPQEAVRLDRIGGKTVSLVDGRNGELLETLDFDR
                     ALREAFVGAVYMHNAATWVVRELDLESLRAVADRQDVDYFTVAHQERQVEMGGDPEAT
                     LAGRDGSWSAGLGGLSITHTLTHYLKKRFGIVIGSVPLDLPARTLETTGFWIDLPGPK
                     IPCVNDLPGAMHALEHAVVGISPLVISCDPDDLAGFSTMMAPHTGGPAIFVYDGYDGG
                     IGLSERAFRDLPRLLAVALDILEGCRCLAGCPACCLSPRCGNNNQPMDKQGAIVLARR
                     ILGL"
     gene            complement(3793..>4893)
                     /locus_tag="PKH54_07955"
     CDS             complement(3793..>4893)
                     /locus_tag="PKH54_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOC99864.1"
                     /translation="IGGYFDPAARLNAVSARSVRSGGDLSGHDAAAMMEVFRERYPGR
                     DVADIIRREIDRATVVSDRDLIQVCRPTDREVRVVPRQQAIRAISSSLWLIPGIRDKV
                     ACDLCGNGNRTIASLSSHHRFGCAAGRLERSLERGDAARVSAIISGRGGAAHPLNLAV
                     SAWFESGDILFLDIETGGLFGGSPIVVVGLASACDPTRPDHAVVKILCATTPDSEAEL
                     IRKTVEEIEAHKVLVSFNGKSFDLPYVSQRAAYYGTVIRNDPVHFDLLGYSRRLFSGK
                     TSDCRLSTIAREVLGMDRDLDVPGSLVPVFYENYLRDPAKNAGLLAAIAIHNCYDMDQ
                     TIQLFREHVSRLKDLSGETGFDGFSNSTEGVA"
BASE COUNT         1145 a         1512 c         1338 g          898 t
ORIGIN      
        1 caggttgaac aggaatccgc tcatcatgat cgaaaccggc atgtcgttcc tgtttccacg
       61 cagacggaac ggaaagatga acgtcctgtt gatgtaatgg agttcgtaca tgcacagaaa
      121 gacaatcgga agaatactgg acgtccgttc accgacaaaa aagaacagcg cgaacaggat
      181 gggcgccggg gcctccatga ttacccagcc gacggtatcg ttgatgcgcg gcccccaccc
      241 cttcctggtg tgacgtccat aaggagcggt tacgaacagg agtagcggaa aaaccagggc
      301 ggccattccc aggaacacca ccatcaacca gaaatgaaaa accttctcgc cggcaagtat
      361 cgaagaaaag tccataagat cctccgccta tatcatcccg acttccttga accagtcgtg
      421 gatatccttc aacgattcgt cgagcggacg tggattgaag ccaagttccc ttgccgcctt
      481 gtcgtgcctg atatcactgt tggcgcgcag cgcattcagg gattccggcg tgaacagggc
      541 cggcagaccg gttattcggg atacgagaag ggccgctggt gcgacagccc tggcaagcca
      601 catcggacac atgaacttcg gggccggacg accgcaaacc ttttcaaacc tggtggccag
      661 ctccctgaac gtcgtgtagt gaccgccgag gaggtaaagt tccccggtcc gcccctagct
      721 ttcggccgca atggcggccg ccgcgacgtc acgtgcgtca acccagtcaa atccgccctg
      781 gacaccgccc ggcaatttac ctgtcgcaag gcccaggaat acagttccca tgcgcgactt
      841 cttccagtcc agcggaccga tgacaccggt cggacacacg atcacagcat caagcccctt
      901 ttccacggcc ttcaggattt caagctcgcc gtcacccttt gatatgtcgt acgccggtgt
      961 tccgcgtcca ccggaacgct ccctggtttc atccaccggc ttgtccagat cggtattgat
     1021 gttgcgggcg tgaatcgaac tgaaatgcac cagccgcctg accccgcact ggatacaagc
     1081 ttcggcgaca ttggccgcgc cgccgacatt gattgcgcgg acaagaccac cttcgggccc
     1141 gacaatggaa atcctggatg ccaggtgata cacgacgtcg gccccggaaa atgccctctt
     1201 caggctttcc agatcccgga catccccgca cacgcgttcg acatcaagac cgtccagggc
     1261 agccgtgtta tcgtcatgtt cccagaccag gcaacgaacc ttccggcctt ccgccagaag
     1321 caaccttaca aggtttccac cgatatgtcc ggtcgcaccg gttactaccg tgagcatccc
     1381 atcccccgaa aaagccaggc aaacgtcctg aacggccaag atccacatca agtgatcccg
     1441 caccttgccg gacgcggcaa cgcaataaat caggccgttc gttacgtcaa caaaaaactt
     1501 ccccgcccgc cgatgccgcc gggacaaggc aacaccatca cataaattga aaaccaagcc
     1561 cttaaagacc aaggatccgc ctggccagga caatggcgcc ctgcttgtcc atcggctgat
     1621 tgttgtttcc acagcggggc gacaggcaac aggccgggca gccggcgaga cagcgacagc
     1681 cttcaaggat atccagcgca accgccagca gacgcggtaa atccctgaac gcacgttcag
     1741 aaagaccgat accaccgtca tatccatcat aaacgaatat ggccggtccc cctgtatgcg
     1801 gtgccatcat tgtcgaaaaa ccagcaaggt catccgggtc gcaggaaatc acgagcgggg
     1861 aaatgccgac gaccgcatgc tccagcgcgt gcatggcgcc gggcagatca ttgacgcagg
     1921 gaatctttgg accgggcaaa tcaatccaga agcccgtcgt ttcaagggtg cgcgcaggaa
     1981 gatcgagcgg gacagacccg ataacgattc caaaccgctt cttcaggtaa tgcgtcaacg
     2041 tgtgcgtgat ggacaggccg ccaagtcctg cggaccatga gccgtcccgg cctgcaaggg
     2101 tcgcttccgg atcgccaccc atttcgacct gacgctcctg atgggcgacc gtgaaataat
     2161 cgacatcctg tcgatcggca acggcccgca acgattccag atccagttcc ctgacaaccc
     2221 atgttgcggc gttgtgcata tatacggcgc cgacgaacgc ctcgcgcagt gccctgtcaa
     2281 aatcaagggt ttccagaagt tcgccgttac gaccgtcaac cagcgatacc gtcttgccgc
     2341 caatcctgtc cagccgaacg gcttcctgtg gccttaccga accgccgtat atgaacccgt
     2401 tgacggtttc ccgaagcagg cccgcgccgg caagggactt cgcgatcccg aaaagatggc
     2461 tgtcggcttc atcaaccttc aagggaagct cggatgccgc acacagcgcg tgacccgcaa
     2521 gaatgtgggg gttgtccaga tcgatggtcg cacgctcaaa cgccctgtcg gtcagaattt
     2581 cggtgtgcga ggcaaggtac tggtcaagga tgtcgccacc ggctacgaaa attgccgcgc
     2641 ttgcgccgcc ccttcgaccg gttcggccag cctgctgcca gaacgcgctg atgcttccgg
     2701 gatagcccga aacaacgacc gcgtccagat cgccgatatc gattccaagt tccagggcat
     2761 tggtgctgac gacaccccgg atggaacctt cacgaaacgc ctgttcaagt tgacgcctct
     2821 gtgctggaag atatccggcc ctgtacggcg atatcggcag gccgccgaat cgatccgccg
     2881 ccaagcttgc cacaagctcg gccgttcgac gcgaccttgt aaagcagagt gtcttgattc
     2941 cccgcctgac cagcccggcc acgatatccc gtgtccttgc ggccggggat ccaccgccct
     3001 caagaccgcc gttcacgacg ataagcgcct tttcaccgga cggagaacca tcgtcatcaa
     3061 tgcagaccgc gtcctgtccg gtaagcgcaa gggcgtgcgg gcctgcattg gcgatcgagg
     3121 cggaagccat gataaaacgg ggcgatgcgc catagacgga cgccacccgc acaagacgcc
     3181 ggattacctg cgcgacgttt gatccgaata cgccggtata gcggtgtgcc tcgtcgatga
     3241 ccacgtaccg caggttcagg aagaatcgcc gccaaagatg gtgatacggg agcgtttcat
     3301 ggatttccca cggatttgaa aggatcacgc gggaaagctg tcgtatccgg gaccttgacg
     3361 cccttggggt gtccccgtcg tatatcgccg cgtcgatctg tacaccggac gcatcctgaa
     3421 ctgccgaaaa cacgtcgagc tggtcgttgg taagcgcttt caatggataa agaaagagtg
     3481 cgcacgcatc ggggtcggtc gcaagttcat ggagtacggg cagagtgaag gcaagcgtct
     3541 tcccggaagc cgtggcggtc gaaagaatga cgttgcgtcc ggccatcgac accgagcagg
     3601 catgggcctg gtgtgagtac agcgactcga ttccggaacg cgacaggtaa tcaaccacat
     3661 caggatgaag accaggcgga tgcggggcca gtcttgcttc acgggccggc agcgaggttg
     3721 aaagaaccac atccccttcc ggaatggatt cattttgaac gtaaagaatg gcatcgcgaa
     3781 tctggctgat tgtcacgcaa caccttccgt tgaattcgag aaaccgtcaa atcccgtctc
     3841 gcccgacaag tccttgagac gggaaacgtg ttcccgaaac agttggatgg tctgatccat
     3901 gtcataacaa ttgtgaatgg cgattgcggc aagaagcccg gcgtttttgg ccggatccct
     3961 taaatagttt tcgtagaaga ctggaacaag ggatcctggg acgtccagat cccgatccat
     4021 tcccaacacc tcgcgggcga tggtcgaaag ccggcagtcg gatgtcttgc ccgaaaaaag
     4081 acgcctcgaa tacccaagca gatcaaaatg aacgggatcg ttgcggatga cggtcccgta
     4141 atatgccgcc ctttgagaaa catacggaag atcaaacgat tttccgttaa aggacaccag
     4201 caccttgtgt gcttcgattt cctcaacggt cttgcggatc agttcggctt ccgaatccgg
     4261 tgtcgtggca caaagaatct taacgaccgc atgatctggg cgggtagggt cgcacgcgga
     4321 ggcgagaccg accacgacta tcggactacc cccgaaaagc ccgccggtct cgatatcaag
     4381 aaacagaata tccccagact cgaaccaggc cgacacggca aggttcaacg gatgggctgc
     4441 acccccgcgg ccgcttatta tcgccgatac ccgtgcggcg tccccgcgtt ccaggctgcg
     4501 ttccagcctt ccggcagcac acccgaacct gtggtgcgat gacagggaag cgatcgtcct
     4561 gttaccattt ccacacagat cacaggcgac tttgtcccga atcccgggga tcagccacaa
     4621 agacgaggaa atggcccgaa tcgcctgctg ccggggcacc acgcgaacct cgcgatcggt
     4681 cggccggcaa acctggatca ggtcccggtc cgataccacg gtcgcacggt caatttcccg
     4741 tcgaatgatg tcggcaacat cgcgtccagg gtacctttcc cggaaaactt ccatcatcgc
     4801 ggcggcgtca tgcccggaca aatccccgcc cgaccggaca gaccttgcgg aaacagcgtt
     4861 caaacgggcg gccggatcaa aatagcctcc gat
//
LOCUS       DAOPPA010000231         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18863_length_4887_cov_4.042632, whole genome shotgun sequence.
ACCESSION   DAOPPA010000231 DAOPPA010000000
VERSION     DAOPPA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18863_length_4887_cov_4.042632"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKH54_07960"
     CDS             complement(<1..647)
                     /locus_tag="PKH54_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOC99865.1"
                     /translation="MGCRRRVFLIIALVLSVPLSASAAFEPQSAMRRDWERHLELPWQ
                     MAPDQPFRPARELVSDRTGPRPVVFGYLPYWEFGDAVLHMDLLTHIAYFGVSIDGQGR
                     LQDSRHWNTTAMDELVAAARASGVKVVLTVICFDSTAMDSLLNSTDYRAAAISSITDL
                     VVAGDGDGVNLDFEGLPKTAKDGFVQFVSDLKASLDAALGDSSLTLAMPAVDWKGA"
     gene            822..1304
                     /locus_tag="PKH54_07965"
     CDS             822..1304
                     /locus_tag="PKH54_07965"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HOC99866.1"
                     /translation="MKITHEHVVSIPKSRVLEFYMDDDYYRGQLKNTGALTVEILETA
                     AIPGGGKKRKAQVTAPSRVPQMLRTSDTDQYIDTNTVDVAGSRMFYEITPSMFADQFK
                     LAGGIEFIDNGAGTRLVFSTEVEIRLPLIGKKLEKQAIDEAENEVGKQVEFLKSWAAK
                     "
     gene            complement(1439..1888)
                     /locus_tag="PKH54_07970"
     CDS             complement(1439..1888)
                     /locus_tag="PKH54_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOC99867.1"
                     /translation="MVETARVVEVGPSGSVTVEVTRHEACGTCAARGACHPGGVKTGR
                     VVAVCGLPCTVGDTVEISISDSAVRFAVLWAYVVPAGLLLASLSAVWYALSGTELEGS
                     RDIIAGIAGLAGAGLGFLIMKLVNGRIRRPGTKAHERFTIRVVRILD"
     gene            complement(1894..2820)
                     /locus_tag="PKH54_07975"
     CDS             complement(1894..2820)
                     /locus_tag="PKH54_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOC99868.1"
                     /translation="MMDFVALVTAGGVLAGVGAAAAALLAVASRVFAVQVDERITAIA
                     AVLPGANCGGCGFPGCSGYAAAIVNKGVDPSLCAPAGADAAKKIGAIMGVEVPDREKV
                     IARVRCGGGHDKCKPRFEYRGLKTCRGAVMLNEGGAKSCPYGCEGLGDCVDVCVFDAI
                     RMGEDGIPVVDMEKCTGCGKCVTACPKDVIELQACKGPVAINCNSQWKGKEVKNVCEV
                     GCITCLACVKACPWEAITMKGNIPVIDYDKCHACGLCVDSCKPGSIVILRAIDEAAKE
                     EGQKIIADRKAAAAAAKAAAAAAAAKPAPVQE"
     gene            complement(2829..3410)
                     /locus_tag="PKH54_07980"
     CDS             complement(2829..3410)
                     /locus_tag="PKH54_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOC99869.1"
                     /translation="MGLLTLIIWTVFVNNVILSQFLGICPFMGVSKKLDSAIGMSAAV
                     LFVIVLATVITYLLQFGVLVPLRLEYLQTIMFILVIAALVQFVEMVIRKVSPALFEAL
                     GIFLPLITTNCAVLGTAIMATQKDFNLLQAVVFAVGISLGFALVLLLFAAIRERLDLE
                     TSTPRYMKGMPIALVTASILSLAFLGFSGMVKM"
     gene            complement(3412..4014)
                     /locus_tag="PKH54_07985"
     CDS             complement(3412..4014)
                     /locus_tag="PKH54_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOC99870.1"
                     /translation="MADSSLFKEFAKGTVKENPLLISLLGMCPALAVTTSLTNAVGMG
                     AAVIFVLTMSNIVVALLRKIIPDAVRIPAFIVIIASFVTIVDLSMAAWVPSLSKSLGL
                     FIPLIVVNCIILARAEAFAYKNGVARSIMDGVGMGLGYTLGLVSVAFFRELLGNGTLF
                     GIGVLGEAFKANSVLVMILPPGAFLTLGLIVGFMNRIRRV"
     gene            complement(4007..4606)
                     /locus_tag="PKH54_07990"
     CDS             complement(4007..4606)
                     /locus_tag="PKH54_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOC99871.1"
                     /translation="MKEARYVIVLTGISLVCATLLGAVYQVTEEPIRQAKNKAVVEGI
                     QKVLPEGTKADADNKVIVDESNPLSPVIYPAVRDGVVVGAAVKATSTAGYGGNLVVMV
                     GIEGVPGSGLKVFNTSVLDMSKETPGLGTKVAEPAFAGQWNNRLVTEKALAVKKDGGD
                     VVAVSGATITSRAFTDCVNQAINAWTKLGPAIVKGGSNG"
     gene            complement(4603..>4887)
                     /locus_tag="PKH54_07995"
     CDS             complement(4603..>4887)
                     /locus_tag="PKH54_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOC99872.1"
                     /translation="PAQYASPLTHVLTGGLMLGAIFMATDMVTSPISFGGRVVFAVGC
                     GLLTGLIRLFGGYPEGVSFAILLMNAATPLIDNLMAPRTFGTPRKAEVAK"
BASE COUNT         1092 a         1525 c         1418 g          852 t
ORIGIN      
        1 gcccccttcc agtcgacggc aggcatcgcg agtgtcaggg acgaatcccc gagggccgcg
       61 tccagggatg ccttcaggtc ggaaacgaac tgaacgaaac cgtcctttgc ggtcttcgga
      121 aggccctcga agtccaggtt gacgccgtcc ccgtcaccgg caaccacgag gtcggttatc
      181 gaggatatgg cggcggctct gtagtcggtc gagttcagga gcgaatccat cgcggttgaa
      241 tcgaaacata tgacggtaag gacgaccttg acgccggatg cacgggcggc ggcaaccagt
      301 tcgtccatcg ccgtggtgtt ccagtgcctg gaatcctgca acctgccctg cccgtcaatc
      361 gatacgccga agtacgcgat atgggtaaga agatccatgt ggagcacggc gtcgccgaac
      421 tcccagtacg gaagatagcc gaacacgacc ggacggggcc cggtgcgatc ggaaacaagc
      481 tcgcgagccg gtctgaacgg ctggtccggc gccatctgcc acggaagctc caggtggcgt
      541 tcccagtcgc gcctcatcgc ggattgcggc tcaaaggccg cactggcgga cagggggaca
      601 gaaaggacaa gcgcgatgat caggaaaacg cgtcgccggc agcccattcc gaatcctcct
      661 ttcgccacca gatccgaacc gccccgacag gttcggaata tcaagcgtgc catccgaatc
      721 aaacgactgc aaccccgcgc gaaggcgggg tgttgcaaat tacggccaat gcgaaggaaa
      781 atcgcaacct gcatggccgg tcgagccatc ttggaggaac catgaagatt acacacgaac
      841 atgttgtttc cattcccaaa agccgcgtcc ttgaattcta catggacgac gactactacc
      901 gcggacagtt gaagaacact ggcgccctga ccgtcgaaat cctggaaacg gcggcaatcc
      961 caggcggcgg caagaaaagg aaggcccagg tcacggcccc gtcacgggtt ccacagatgc
     1021 tgcgcacgtc cgacacggat cagtacatcg acacgaacac ggtggatgtt gcgggatctc
     1081 gaatgtttta cgagatcacg ccgtcgatgt tcgcggatca gttcaagctg gccggtggaa
     1141 tcgagttcat cgacaacggg gccggaacaa ggctggtatt ttcgaccgaa gtcgagatca
     1201 ggctgccgct gatcggcaag aagctggaaa agcaggctat cgacgaggcc gaaaacgaag
     1261 tcggaaagca ggtcgagttc ctgaagtcct gggccgcgaa gtaaccgcag ttaccccgaa
     1321 ggccccagtc agcacacatc gagggacgga cggttgcttc ccaggcgggc gaattttcat
     1381 tcgcccttac gattcgggga ggaccggggc ggacggttgc tccccgcccc caaatttatc
     1441 aatcaaggat tcttacaacc cggatcgtga atcgttcatg ggctttcgtg cctggcctgc
     1501 gaatcctgcc gttgaccagc ttcattatca ggaagccgag cccggccccg gccagcccgg
     1561 caattcccgc aataatgtct cgactgcctt caagctcggt tccggagagc gcgtaccaga
     1621 ctgcggaaag cgatgccaga agaagcccgg ccggaacaac gtatgcccag aggacggcaa
     1681 agcgcacggc cgagtccgat atggaaattt cgacggtatc cccgaccgtg cacggcaatc
     1741 cgcagaccgc caccacgcga ccggttttca ctccgccggg gtgacatgcg ccgcgtgccg
     1801 cacaggtgcc gcaggcctcg tgccgggtaa cttcaacagt caccgaccct gatggcccga
     1861 cctcgacgac cctggcggtt tcgaccatga ctgctattcc tggaccgggg cgggcttcgc
     1921 cgccgcggcg gctgccgcgg ccttggccgc tgcggccgcg gccttgcgat cggcgatgat
     1981 cttctggcct tcttccttcg cggcttcatc aatggcacgc aggatcacga tgcttccggg
     2041 cttgcaggaa tcgacgcaaa ggccgcaggc atggcacttg tcgtaatcga tgacagggat
     2101 gttgcccttc atcgtgatgg cttcccacgg gcatgccttg acgcacgcaa gacacgtgat
     2161 gcatccgacc tcgcagacgt tcttgacttc cttgcccttc cactggctgt tgcagttgat
     2221 cgctaccggg cccttgcacg cctgcagttc aatgacgtcc ttcgggcagg cggtcacgca
     2281 cttgccgcag ccggtacact tttccatatc gacaacggga atcccgtctt cgcccatacg
     2341 gatggcgtcg aacacgcaga catccacgca gtcgcccagg ccctcgcatc cgtacggaca
     2401 ggacttggcg ccgccttcgt taagcatgac cgcgcctcgg catgtcttga gtccgcggta
     2461 ctcgaaacgg ggcttgcact tgtcgtgacc gccgccgcac cggacgcggg caatgacctt
     2521 ttcgcggtcg ggcacctcga cgcccatgat ggcgccgatc tttttcgcgg catcggcgcc
     2581 ggcaggggcg cacagggaag gatcgacacc cttgttgacg atggccgccg catagccgga
     2641 acagcccggg aaaccgcacc cgccgcagtt tgcccccgga agaaccgccg caatcgcagt
     2701 aatcctttcg tcaacctgga ccgcgaaaac acgcgaagcg accgccagca gggcagccgc
     2761 ggcagcgccc actccagcca gcacgccacc cgcggttaca agcgcaacaa agtccatcat
     2821 tcacctcact acatcttgac catgcccgag aacccaagga acgccagcga gaggatggat
     2881 gccgtcacca gcgcgatcgg catgcccttc atgtagcggg gcgtggaggt ttcaaggtca
     2941 agacgttcgc gtatggccgc aaacagcagc agaacaagcg cgaagccaag ggaaatgccg
     3001 accgcgaaaa cgacggcctg caaaagattg aagtccttct gggtcgccat gatcgcggtg
     3061 ccgagcaccg cgcagttcgt ggtaatcagg ggcaggaaga tgccaagtgc ttcaaaaagc
     3121 gccggggaaa ccttccggat caccatttca acgaactgga caagcgccgc gataaccagg
     3181 atgaacatga tcgtctgcag gtactcaagc ctcaacggca caagaacgcc gaactgcagc
     3241 agataggtaa tcacggtggc gaggacgata acgaaaagaa ccgccgcgct catgccgatg
     3301 gcggagtcga gcttcttcga aacgcccatg aacggacaga ttcccaggaa ctgggacagg
     3361 attacgttgt tgacgaatac cgtccagata atcagcgtaa ggagacccat gtcaaaccct
     3421 ccttatccgg ttcatgaagc cgacgatcag cccgagcgtc aggaaggccc ctggcggaag
     3481 aatcatgacc agcaccgaat tggccttgaa agcctcgcca agcacgccga tcccgaacag
     3541 tgtcccgtta ccaagcagct cacggaaaaa tgcaacggac accagaccca gcgtatatcc
     3601 gaggcccatt ccaacaccgt ccatgatcga cctggcgacg ccgttcttgt acgcaaacgc
     3661 ctcggcgcgg gccaggataa tgcagttgac gactatcagc gggatgaaca ggccaagtga
     3721 cttcgacagg ctgggaaccc aggcggccat cgaaagatcg acgatcgtga cgaacgacgc
     3781 gatgatgacg ataaatgccg gaatacggac tgcgtccgga atgatcttcc gcaacagggc
     3841 cacaacgata ttcgacatgg taaggacgaa gatgacggcg gcgcccatgc cgaccgcatt
     3901 cgtcagggat gtcgtgaccg ccagcgccgg acacattccc agcagggaaa tcagcaacgg
     3961 gttttccttg accgttccct tggcgaattc cttgaacagg gatgaatcag ccatttgaac
     4021 ctcccttgac gattgccggg cccagtttcg tccatgcgtt gattgcctgg ttcacgcagt
     4081 cggtaaaagc cctggaagtg atggtggcgc ccgaaaccgc aacaacatcc ccgccatcct
     4141 tcttgacggc cagggccttt tcggtgacga gcctgttgtt ccactgaccg gcgaatgccg
     4201 gctcggccac cttggtgccc agtcccggcg tttccttgga catgtccaga acgctggtgt
     4261 tgaagacctt cagcccggat ccgggcacgc cctcgatacc gaccatgacg accagatttc
     4321 cgccgtatcc cgcggtgctc gttgccttga ccgcggcccc gacgacaacg ccgtcacgaa
     4381 cggccggata gatgaccggt gaaagcggat tggattcgtc aacgatgacc ttattgtcgg
     4441 catccgcctt cgtgccttcg ggaagcacct tctgtatccc ttcgacgacc gccttgttct
     4501 tcgcctggcg aatcggttcc tcggtgacct gatacacggc cccgagaagg gtcgcgcaga
     4561 caagcgatat gcctgtaagg acaatcacgt accttgcttc cttcatttcg ccacctccgc
     4621 cttgcgtggc gtcccgaaag tcctgggggc catcaggtta tcgataaggg gcgtcgcggc
     4681 attcatcaga aggatggcaa acgacacgcc ctcgggatat ccgccgaaca ggcgaatcag
     4741 gccggtaaga aggccgcagc ccacggcgaa caccacgcgt ccaccgaaac tgattggcga
     4801 agtgaccatg tcggtcgcca tgaatatcgc gcccagcatc aggccgccgg tcaggacatg
     4861 ggtcagggga cttgcatact gcgccgg
//
LOCUS       DAOPPA010000232         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18864_length_4887_cov_3.773593, whole genome shotgun sequence.
ACCESSION   DAOPPA010000232 DAOPPA010000000
VERSION     DAOPPA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18864_length_4887_cov_3.773593"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="pyrR"
                     /locus_tag="PKH54_08000"
     CDS             <1..444
                     /gene="pyrR"
                     /locus_tag="PKH54_08000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOC99873.1"
                     /translation="CLIGIQTGGVYVAQRLHKAMEAVGFKDICFGRLDITLYRDDIFT
                     ALQQPVVRSTDIRFDLTGKNVILVDDVLYTGRTIRAALDALVDFGRPRRVRLAVLVDR
                     GLREYPIQADFAGRTVNTDANETVQVELVEMGFERDRVVVYERQS"
     gene            444..1385
                     /locus_tag="PKH54_08005"
     CDS             444..1385
                     /locus_tag="PKH54_08005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOC99874.1"
                     /translation="MENPNTLNHLLALEGLSKETVTSILDHAVGFKEVSKRRIKKVPP
                     LRGVTVVLFFIENSTRTRISFEVAAKRLSADTLNFSASTSSLSKGETLLDTAKNLMAM
                     QPDIIVMRHFAPGAAKFLSERIPAGIVNAGDGPHAHPTQALLDMYTMRERKGRLEGLE
                     VAIVGDIAHSRVARSNIHGLLTMGAKVRLAGPRTMLPTMIDRFPVSVHDRIEPAIEGA
                     DVVMMLRIQKERMGKDLFPSDREYARVFGLDSQKLKLAKPDCLVMHPGPMNRGVEITP
                     EVADSTQSVILEQVENGVAVRMAVLFMVARHLGVSEE"
     gene            1397..2683
                     /locus_tag="PKH54_08010"
     CDS             1397..2683
                     /locus_tag="PKH54_08010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOC99875.1"
                     /translation="MRTRIVNCQIFDPATGGDAFPGWVEFENGVITAVGKGQAPAPSA
                     GAEITDIDGQGFTLAPSFVDMYANFCEPGYEYRETVASGSRAAASGGYTAVCLRPDTD
                     PIIQRSDVVRFLLERGVFANMVDIFPIGALSMNMEGQTMAEIGEMAAAGARLVGEGNT
                     FVKRTGFLRRCMEYAGNFGLVVMLTNEDPSLAEGPCHEGFMSTSRGLKCTPVQAEEIA
                     VARHIALAELTGTPTHLVKISSAAGVRLIRDARARGINITASVSIHNLALNEDANAGY
                     DAMTKVWPPLRAEEDRLALLAALEDGTIDIVVSDHDPHVIEGKEVEFDLATVGASSIE
                     LTFGVLNTLVERGELKFATVIRALATAPRKIMHLDGGVISAGMPADLVLLDRKAVRKV
                     CGAELVSQGVNTPFDGTDLAGCPVMTFRRGRMTFAA"
     gene            2721..4013
                     /locus_tag="PKH54_08015"
     CDS             2721..4013
                     /locus_tag="PKH54_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC99876.1"
                     /translation="MEIRDPIHGPITTSQAEMDLIAHPLFERLRGIRQLDFSEYTFPG
                     ATHTRYIHSLGTMHLAGLAFDMMARDLGQFVPAVEMPRLRQTVRLAGLLHDLGHPPMS
                     HTGERLLPDLSGRQIGNACCCGHTDHETLTRYLLLNSDIRGLVRDWFADNGVLAEHVE
                     AVLSPVDEDVDAAPFCFGGVNVLTALRQLISGELDVDRMDYLLRDSYFTGVAYGRFDK
                     DWLLSHIGATVEDGRAFMTLEANAVFTLEDFLLSRHHMFLMVYSHPRTQTYHRLLSRF
                     LDATGLRLPSSLQGLAAFDDAWLMGELRKSNHPDAIRICHHDVPRLIFEAWGDEADRL
                     ASMQGRIEAALPEGVMVQDSNLAFSRYFSRGQRTSDAPTLRVRQVTPGGLSRILPVEQ
                     YSDMYERQARPRRSIRIFGDTGLITQVETVIRDLARLG"
     gene            4032..4835
                     /locus_tag="PKH54_08020"
     CDS             4032..4835
                     /locus_tag="PKH54_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC99877.1"
                     /translation="MARYNDSLQAQLDAAQEMLNANLLEDAWIVLNRLDSAYPDSPEI
                     IATMGDAALKVGDFEFAYEMFDRVIELEPVWSEAWSARARCRLEMCEFETARADLEHA
                     IALDPANPEAHYCIGILAEFDNDEVQARHEFAQAAMIDGEHFIIPFRTATAVDFERLV
                     LKVIGKLKVPAGKGRAIKFSVHELPSVVFCAETGLSPLITFYRAEPDEKNPNPPYLFF
                     RRNIERVSRNIDDIEIEIDAVLGGDFEYADGYAPSAPSAANQHDTEDLH"
BASE COUNT          938 a         1577 c         1490 g          882 t
ORIGIN      
        1 tgcctgattg gcatccagac cggcggcgtg tatgtcgcgc agcgcctgca caaggcgatg
       61 gaggcggtcg gcttcaagga catctgcttc ggcaggctgg acatcacact ttaccgcgac
      121 gacatcttca ccgcgctgca gcagccggtc gtccgcagta ccgacatcag gttcgacctg
      181 acgggcaaga acgtcatcct ggtcgatgac gtgctttaca ccggccggac gatccgcgcg
      241 gcgctggacg cactggtcga tttcggtcgc ccgcggcggg tgcgcctggc cgtcctggtt
      301 gaccgcggac tgcgggaata cccgattcag gcggacttcg ccggcaggac cgtcaacacg
      361 gacgccaacg aaaccgtcca ggtcgaactt gtggaaatgg gctttgaacg cgaccgcgtg
      421 gtcgtctatg agagacagtc ctgatggaaa atcccaacac gctgaatcac ctgctggccc
      481 tcgagggcct gtcaaaggaa accgtgacct cgatcctgga ccatgccgtc ggcttcaagg
      541 aagtcagcaa gcgccggatc aagaaggtcc cgccgctgcg tggcgttacc gtcgtgcttt
      601 tcttcatcga aaactcgacg cgcacccgca tcagctttga agtcgcggcc aagcgcctgt
      661 cggccgacac gctgaacttc agcgcatcga cgtcgtcgct gtccaagggc gaaaccctgc
      721 tggataccgc caaaaacctg atggcgatgc agcccgacat catcgtcatg cggcacttcg
      781 cccccggcgc ggccaagttc ctgtcggaac gcatcccggc cggcatcgtc aacgccggtg
      841 acggacccca cgcgcacccg acccaggccc tgctggacat gtacacgatg cgtgaaagga
      901 agggtcgcct ggaaggcctg gaagtcgcca tcgtcggcga catcgcccac agccgcgtgg
      961 cccgttccaa catccacgga ctgctgacga tgggcgccaa ggtccgcctg gccggcccgc
     1021 gcaccatgct gcccacgatg atcgaccgct tcccggtcag cgtccatgac cgcatcgagc
     1081 ccgccatcga gggcgccgac gtcgtcatga tgctgcgcat ccagaaagaa cgcatgggca
     1141 aggacctgtt cccgtccgac cgcgaatacg cccgcgtctt cggcctggat tcccagaagc
     1201 tgaagctggc gaagccggac tgcctggtga tgcacccggg cccgatgaac cgcggcgtcg
     1261 aaattacgcc cgaagtggcc gattcgaccc aatccgtgat cctggaacag gtcgaaaacg
     1321 gcgtggccgt ccgcatggcg gtccttttca tggttgccag acaccttggc gtgtcggaag
     1381 aatgatggtg acggttatgc gtacgcgtat tgtgaattgt cagattttcg atccagcgac
     1441 cggcggcgac gccttccccg gctgggtcga gttcgaaaac ggcgtcatca cggccgtcgg
     1501 caagggccag gcccctgccc cgtccgccgg agccgaaatc accgacatcg acggtcaggg
     1561 atttaccctg gccccgtcgt tcgtcgatat gtatgccaat ttctgtgagc ccggttacga
     1621 ataccgtgaa acggttgctt ccggttcacg cgccgcggca tcgggcggtt acaccgcagt
     1681 ctgcctgcgc ccggacaccg acccgatcat ccagcgtagc gacgtcgtcc ggttcctgct
     1741 tgaacgcggc gtattcgcga acatggtcga tatattcccc atcggcgccc tttccatgaa
     1801 catggaaggc cagaccatgg ccgaaatcgg cgaaatggcc gcggccggcg cacgcctggt
     1861 cggcgaaggc aacacgttcg tgaagcggac cggattcctg cgccgctgca tggaatacgc
     1921 cgggaacttc gggctggtcg tgatgttgac gaacgaggac ccttcacttg ccgaaggccc
     1981 ctgtcacgaa ggcttcatgt ccacgtcccg cggcctgaag tgcaccccgg tccaggccga
     2041 ggaaatcgcg gtcgcccgcc acatcgccct ggcggaactg accggcacgc cgacccacct
     2101 ggttaaaatc agcagcgccg ccggcgtccg cctgatccgc gatgcacgcg cccgcggcat
     2161 aaacatcacc gcgtccgtgt cgatacacaa cctggccctg aacgaagacg ccaacgccgg
     2221 ttacgacgcg atgaccaagg tatggccgcc actgcgcgcc gaggaagacc gcctggcgct
     2281 gctggccgcg ctggaagacg gcacgatcga catcgtcgtg tccgaccacg acccgcacgt
     2341 catcgagggc aaggaagtcg aattcgacct ggccaccgtc ggcgccagca gcatcgagct
     2401 gaccttcggc gtgctgaaca cgctggtcga acgcggcgag cttaagttcg cgacggttat
     2461 ccgggcactg gcgacggccc ccaggaagat aatgcacctg gacggcggcg tgatttccgc
     2521 cggcatgccg gccgaccttg tccttctgga tcgcaaggcc gtgcgcaagg tctgcggcgc
     2581 cgaactggtg tcccagggcg tcaacacgcc gtttgacgga accgaccttg cgggttgccc
     2641 cgtcatgacc ttccgccgcg gacgcatgac ttttgcggct tgacccggct cggcccccct
     2701 gccccggagg tatctggccc atggaaatcc gtgatcccat tcatggcccg ataacgacgt
     2761 cccaggcgga aatggatctg atcgcacacc cgcttttcga aaggctgcgc ggcatcaggc
     2821 aactcgactt ttccgaatac acgttccccg gggcgacgca caccaggtac atccattcgc
     2881 ttgggacaat gcaccttgcg ggccttgcat tcgacatgat ggcccgggat cttggccagt
     2941 tcgtcccggc ggtcgagatg ccgcgcctgc gccagacggt gcgcctggcc gggctgcttc
     3001 acgaccttgg ccacccgccg atgtcgcata ccggtgaaag gctgctgccg gatctgtcgg
     3061 gtcgccagat tggaaacgca tgctgctgcg ggcacaccga ccatgaaacc ctgacccggt
     3121 acctgctgct taattcagac atccgcgggc ttgtccggga ttggtttgcg gacaacggcg
     3181 tccttgccga acacgtcgag gccgtcctga gtcccgttga tgaggacgtc gatgccgcgc
     3241 ccttctgctt cggcggcgtc aatgttctga cggcgctgcg gcaactgatt tccggcgaac
     3301 tggacgttga ccggatggac tacctgctgc gcgacagtta cttcaccggc gtcgcgtacg
     3361 gccggttcga caaggactgg ctgctttccc acatcggggc aacggtcgag gacggccgcg
     3421 ccttcatgac gcttgaagcc aacgcggtct tcaccctgga agatttcctg ctttccaggc
     3481 accacatgtt cctgatggtt tacagccacc cacggaccca gacgtaccac cggctgcttt
     3541 cgcgattcct ggacgcaacc ggcctgcggc tgccgtcatc ccttcagggc ctggccgctt
     3601 tcgacgatgc gtggctgatg ggggagctgc ggaaatcgaa tcacccggac gcgatacgca
     3661 tctgccatca cgacgtgcca aggctgattt tcgaggcgtg gggggacgag gccgaccgcc
     3721 ttgcgtcgat gcaggggcgc atcgaggccg cattgccgga aggcgtgatg gtgcaggaca
     3781 gcaacctggc cttttccagg tacttcagcc gtggccagcg gacaagcgac gccccgaccc
     3841 ttcgggtgcg ccaggtgacg cccggcgggc tttccaggat acttccggtc gagcagtaca
     3901 gcgacatgta cgaacgccag gccaggccgc ggcgcagcat ccggattttc ggggacaccg
     3961 gacttatcac ccaggttgaa acggttataa gggatctggc ccgcctgggc tgatccaatg
     4021 gatattcacg aatggcacga tacaacgact cgttgcaggc ccagcttgac gcggcccagg
     4081 aaatgctgaa cgcgaacctt ctggaggacg cgtggatcgt actgaaccgc ctggactcgg
     4141 cgtatcccga ttcacccgaa atcatcgcca cgatgggcga tgcggcgctg aaggtcgggg
     4201 acttcgaatt cgcatacgaa atgtttgacc gtgtgatcga gctggagccg gtttggtccg
     4261 aggcgtggtc cgccagggcg cgctgccggc tggagatgtg cgaattcgag accgccaggg
     4321 ccgacctgga acacgccatc gcgcttgatc cggccaaccc ggaagcccat tactgcattg
     4381 gcatcctggc tgaattcgat aacgatgaag tccaggccag gcacgaattc gcccaggccg
     4441 ccatgatcga cggcgagcac ttcatcatcc cgttcagaac cgcgacggcc gttgatttcg
     4501 aaaggcttgt cctgaaggtg attgggaagc tgaaagtgcc ggccggtaag ggccgtgcaa
     4561 tcaaattcag cgtgcacgaa ctgccgtcgg tcgtcttctg cgccgaaacc ggcctttcgc
     4621 ccttgataac gttctaccgg gccgagcccg acgaaaagaa ccccaacccg ccatatctgt
     4681 tcttccgaag gaacatcgaa cgcgtttccc gcaacattga cgacatcgag attgagatcg
     4741 atgcggtcct gggcggtgat ttcgaatacg ccgacggata tgctcccagc gcgccaagcg
     4801 cggcgaatca gcacgacacg gaagatcttc actgaattca gtgggcaatg acctggtacg
     4861 gaatctgggt gatggtccag tcggtcg
//
LOCUS       DAOPPA010000233         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18886_length_4881_cov_3.850601, whole genome shotgun sequence.
ACCESSION   DAOPPA010000233 DAOPPA010000000
VERSION     DAOPPA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18886_length_4881_cov_3.850601"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3408
                     /locus_tag="PKH54_08025"
     CDS             <1..3408
                     /locus_tag="PKH54_08025"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99878.1"
                     /translation="LIDNVCYQDNQQNPEDPCEVCNIQYSKTEFMARFNGDPCDDGVA
                     CTFQDQCISGICSGTEYTCNDDIACTEDTCKGDGTCLFVAIAGYCFIDEECVLENTVK
                     PGNACAACLPAVSKQFWSAYREGEGCDDGSNCTIEDACASGDCIGKYKCDDLMDCTAD
                     SCNEAGECSHVVAENYCAINGNCYFYGDQRGLNQCSRCIPPTSNTSWTATDGFGCSDG
                     DPCTTNDKCQGESCAGTPIVCNDGLECTTDFCQYGLCGTVPVSGWCVIGSSCVAEGTL
                     DPANECQICDPASNPYDWSPYNFVTCDDGQACSANDICFAGLCYGQAYDCNDGVPCTV
                     DQCDGLGGCSYGIKAGECLIDGGCYEALDPNILNPCQDCNPTVSQLQWSSIDGLACND
                     GVSCTSNDQCMDSECVGEAYECNDGLACTSDSCNGDGTCTFSRLGGWCLIDNTCIRDQ
                     TLEPGNICRACLSALEGFDWSDNNDVPCDDGLACTKIDTCMAGSCGGTAYACSDGLDC
                     TTDACDGQGGCIFTQLETSCLIDGRCFANGSPNPDSICQKCDVSVSILSWVPNNGQFC
                     NDFDSCTVNDMCSEGECVGQEYNCLDSVECTLDLCDGSGGCSNPLAPNACLIDGTCYW
                     QGEPRPGIPCQACVPDPLNPDSQYEWTDLNPGAIEICNGIDDNCDGILDPENAPGCLD
                     YYRDEDGDGVGVLEDVKCLCAPLDFYRTLVPGDCDDDEPMVYAGRAEDCDGLDNDCDG
                     VTDPDNTPGCIVFYFDNDGDTWGVEDNSRCLCNPQGKFSTAQFGDCDESRAMVNPAAE
                     EVCNGIDDNCDGRTDPPNLPGCVTYYLDTDKDTFGDATTFKCLCQPTAEFNVTNSSDC
                     NDFSDTVYPGAPERCNMIDDDCSGLPDDADLLELCPHDPATLNNAVMFCEGTCKIRCN
                     GANLSAGQPAWYDLDGSAANGCECQGDALELTYSMSQSEPAPLGSLVDDEALTSYQIA
                     KISSVGAEDWFIVTGIDAPDSTMTEIDSDNYHLDIRFNVNPSNEFVFDVFKEVQIGDQ
                     TQVLQICTSSLNWNHYTDFFTGGVGEGVCSSNYVEACTAPEDLARCLVVEGANAPNRC
                     NACPGWASPGANFCANQTETLLVKVRRKAGASPTCSQYKLEASNGVR"
     gene            3419..4048
                     /locus_tag="PKH54_08030"
     CDS             3419..4048
                     /locus_tag="PKH54_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC99879.1"
                     /translation="MLTEKLLEVTLVGSEWILYILLGLSVLSVAIIVERALFYLANRK
                     AWATLKKRIEPMVHGRRWDEVRQVIDSWPGAEVEVLRAAMDGSEIGPEAADKLVESAM
                     IHEQQRLDRRLAFLGTLGNNAPFIGLFGTVLGIIRAFHDLSGNLQGGANVVMAGISEA
                     LIATAVGLFVAIPAVIAFNYFNKSSLREVNRARAAVNEILACRLAGRDD"
     gene            complement(4045..>4881)
                     /locus_tag="PKH54_08035"
     CDS             complement(4045..>4881)
                     /locus_tag="PKH54_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC99880.1"
                     /translation="PGDVVLCPSPGYPPYNRGTLFAGGEPWYYPVTMENGFLPNLDAL
                     PAEVLARARMIWVNYPNSPSGTVAPDDFWPALIDWAHSHRLIIGSDEAYTEVWFNRPP
                     KSILEFGRDGIIVFQSLSKRSNMTCHRVGWVCGDPRLVSLFKKLKTNIDSGTATFIQD
                     GAVAALSDQAHVDAMREVYRAKRDILIPALRDVGCVVPDSEATLYLWPKVPEGMDGLE
                     FATRLLSRDVAIVCTPGAALGEPLADGTSPGRFHARFALVPSVEDCCRAAERIRKAFG
                     RI"
BASE COUNT         1012 a         1510 c         1456 g          903 t
ORIGIN      
        1 cttatagaca acgtctgcta ccaggacaac cagcagaatc cggaagaccc gtgcgaggtc
       61 tgcaatatcc agtactcgaa gacggaattc atggccaggt tcaacggcga tccctgcgac
      121 gatggcgtcg catgcacatt ccaggatcag tgcatttccg gcatctgctc cggcacggaa
      181 tacacatgca acgacgacat cgcctgcacc gaggatacct gcaagggcga tggaacctgt
      241 cttttcgtgg caatcgcggg atactgcttc atcgacgagg aatgcgtcct tgaaaacacg
      301 gtgaagccgg gcaacgcctg cgccgcctgt ctgcccgcgg taagtaagca gttctggtcg
      361 gcgtaccgcg aaggtgaagg atgcgacgac ggctccaact gcacgataga ggacgcctgc
      421 gcgtccggtg actgcatcgg aaaatacaag tgcgacgacc tgatggactg taccgcggat
      481 tcgtgcaacg aagccgggga atgctcgcac gtggtggccg aaaattactg cgccatcaac
      541 ggcaactgct atttctacgg ggatcagcgc ggcctgaacc agtgctcccg ctgcattccg
      601 ccgacaagca acacatcctg gacggcgacc gacggattcg ggtgcagcga tggcgatcca
      661 tgcaccacga acgacaagtg ccagggcgaa tcgtgtgccg gcacgccgat cgtctgcaac
      721 gacggccttg aatgcaccac cgacttctgc cagtacggtc tgtgcggaac ggttccggtt
      781 tccggctggt gcgtgatcgg cagttcctgc gtggccgagg gaaccctgga tcccgcgaac
      841 gaatgccaga tctgcgaccc ggcatcgaat ccgtacgact ggtcgcctta caacttcgtg
      901 acgtgcgacg acggccaggc ctgctcggcg aacgacatct gcttcgccgg actctgttac
      961 ggccaggctt acgactgcaa cgacggcgtg ccgtgcacgg tcgatcagtg cgacgggctg
     1021 ggtggatgca gttatggaat caaggccggc gagtgcctga tcgacggcgg ctgctatgaa
     1081 gccctcgatc ccaacatcct gaatccttgc caggactgca acccgaccgt ttcacaactt
     1141 cagtggtctt ccatcgacgg gctcgcctgc aacgacggcg tttcctgcac cagcaacgac
     1201 cagtgcatgg acagtgaatg cgtcggggaa gcctacgaat gcaacgacgg cctggcgtgc
     1261 acgtccgact cctgcaacgg agacggaaca tgtacgttca gcaggctcgg cgggtggtgt
     1321 cttatcgata acacatgcat ccgcgaccag acgcttgaac ccggcaacat ctgccgcgcg
     1381 tgcctttccg ccctggaagg cttcgactgg tcggacaaca acgacgtccc gtgcgacgac
     1441 ggcctggcgt gcacaaagat cgatacctgc atggccggtt cgtgcggcgg taccgcctat
     1501 gcctgcagcg acggacttga ctgcacgacg gatgcctgcg acggccaggg tgggtgcatc
     1561 ttcacccagc ttgaaacatc gtgcctgatt gacggtcgct gcttcgccaa cggatcaccg
     1621 aacccggaca gcatctgcca gaaatgtgac gtttcggttt caatcctttc ttgggttccg
     1681 aacaacggcc agttctgcaa cgacttcgat tcgtgcacgg tcaacgacat gtgctcggaa
     1741 ggtgagtgcg tcggccagga atacaactgt cttgactcgg ttgaatgcac cctcgatctg
     1801 tgcgacggtt ccggtgggtg cagcaacccg ctggcgccga acgcttgcct gatcgacgga
     1861 acctgctact ggcagggtga accgaggccc ggaattccat gccaggcctg cgtgccggat
     1921 ccgctgaacc cggacagcca gtacgaatgg accgacctga acccgggcgc gatcgaaatc
     1981 tgcaacggga tcgacgacaa ctgcgacgga attcttgatc ccgaaaacgc cccgggttgc
     2041 ctcgactact accgcgacga ggacggcgac ggcgtcggcg tgctcgagga cgtcaagtgc
     2101 ctgtgcgcgc ccctggattt ttatcgaacc ctggttccgg gcgattgcga cgacgacgaa
     2161 ccgatggtct atgccggccg cgccgaggac tgcgacggac tggacaacga ctgcgacggc
     2221 gtcacggatc ccgacaacac tccgggatgc atcgtcttct acttcgataa cgacggggac
     2281 acatggggcg ttgaagacaa ttcacgatgc ctgtgcaatc cacagggcaa gttctcgacg
     2341 gcacagttcg gcgactgcga cgaatccagg gccatggtca atccggccgc cgaggaggtc
     2401 tgcaacggca tcgacgataa ctgcgacggg cgtaccgacc cgcccaacct gccaggctgc
     2461 gtgacttact acctggatac ggacaaggat acgttcggcg acgcaaccac cttcaagtgc
     2521 ctgtgccagc cgacagccga gttcaacgtt accaacagtt ccgactgcaa cgacttcagc
     2581 gacaccgttt atccgggtgc acccgaacgc tgcaatatga tcgacgacga ctgctcggga
     2641 cttcccgatg acgcggatct gctggaactc tgtccgcacg acccggcgac gctgaacaac
     2701 gcggtaatgt tctgtgaagg cacctgcaag atccgctgca acggcgccaa cctgtcggca
     2761 ggccagccgg cctggtacga tctggacggc agcgcagcaa atggatgcga gtgccagggg
     2821 gatgctctgg aactgactta cagcatgtcc cagtccgaac ctgcgccgct gggcagcctg
     2881 gttgacgacg aggcgcttac ctcgtaccag atcgcaaaga tatccagcgt cggtgccgaa
     2941 gactggttca tcgtgacggg catcgacgcc ccggactcga cgatgaccga aatcgacagc
     3001 gacaactatc acctggacat ccgcttcaac gtgaacccgt ccaatgaatt cgttttcgat
     3061 gtcttcaagg aagttcagat cggcgatcag acccaggtac tgcaaatctg cacttcctcg
     3121 ctgaactgga atcactacac cgatttcttc accggcggcg tcggcgaggg cgtctgttcc
     3181 agcaactacg tcgaggcctg cacggcgccc gaggatcttg cacgctgcct ggtggtcgag
     3241 ggtgccaacg ccccgaacag gtgcaacgca tgtccgggct gggcctcgcc aggtgccaac
     3301 ttctgcgcca accagacgga aaccctgctg gtcaaggtcc gccgcaaggc cggtgcttcc
     3361 cccacatgca gccagtacaa gcttgaagct tcgaacggcg tccgctgaga ggttcccgat
     3421 gctcacagag aagcttcttg aagtcacgct ggtcggcagc gagtggatcc tttacatcct
     3481 gctcggcctg tccgttcttt cggtcgcgat cattgtcgaa agggccctgt tctatctggc
     3541 caaccgcaag gcgtgggcca cgcttaagaa gcgcatcgaa cccatggttc acggtcgtcg
     3601 ctgggacgaa gtccgacagg tcatcgactc ctggccaggt gccgaggttg aagtcctgcg
     3661 ggcggcaatg gacggatccg agatcgggcc ggaggccgcg gacaagctgg tggaatcggc
     3721 aatgatccat gaacagcagc gactggatcg aaggctggcc tttcttggaa cgcttggaaa
     3781 caatgcgccg ttcatcggcc tgttcgggac ggttctggga atcatccgcg cgttccatga
     3841 cttgtccgga aaccttcagg gtggcgcgaa cgtggtcatg gccggcatct ccgaggcact
     3901 gattgcgacc gcggtcggtc ttttcgtggc catccccgcg gtcatcgcct tcaattactt
     3961 caacaagagc agtcttcgag aagtcaacag ggcgcgggcg gccgtcaatg agatcctcgc
     4021 atgcaggctt gcgggacgcg acgactagat ccggccaaac gcctttctga tccgttctgc
     4081 cgcgcggcag cagtcttcca cggacggaac cagcgcgaac cttgcatgga agcgaccggg
     4141 actggttcca tcggccagcg gctcgcccag agcggcgccc ggcgtacaca cgatggcgac
     4201 atcccttgaa agcaggcggg tcgcgaactc cagcccgtcc attccttccg gcaccttcgg
     4261 ccacaggtaa agcgttgcct cggaatctgg aaccacgcat ccgacgtccc gaagcgccgg
     4321 tatcaggatg tcacgctttg cccgatagac ttcccgcatg gcatccacgt gggcctggtc
     4381 tgaaagtgcc gccaccgcgc cgtcctggat gaaggtggcc gtcccggaat cgatgttcgt
     4441 cttcaacttc ttgaaaaggg atacgagccg cgggtctccg cacacccagc cgacccggtg
     4501 gcatgtcatg ttcgatcgct ttgaaagcga ctggaacacg attattccat cccggccgaa
     4561 ctccaggatc gacttcggcg gacgattgaa ccagacctcg gtgtatgcct cgtcggaacc
     4621 gatgatcaac cggtgactgt gagcccaatc gatcagggcc ggccagaaat cgtccggggc
     4681 aacggttccg cttggtgaat tcggatagtt cacccatatc atccgggcac gcgccaggac
     4741 ttccgccggg agcgcgtcca ggttcggcag gaatccgttt tccattgtca ccgggtagta
     4801 ccacggctcc ccgccggcaa acagcgttcc ccggttgtat ggcggatacc cgggactggg
     4861 gcacagcacg acgtcgccgg g
//
LOCUS       DAOPPA010000234         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_18999_length_4860_cov_3.735068, whole genome shotgun sequence.
ACCESSION   DAOPPA010000234 DAOPPA010000000
VERSION     DAOPPA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_4860_cov_3.735068"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKH54_08040"
     CDS             <1..209
                     /locus_tag="PKH54_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HOC99881.1"
                     /translation="LVVEIGQAGTAERFDLPAGKSMRVTAETVHRFMSPDDEAVEIFE
                     TSTPELDDVVRLEDVYGREGTSRP"
     gene            237..626
                     /locus_tag="PKH54_08045"
     CDS             237..626
                     /locus_tag="PKH54_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOC99882.1"
                     /translation="MKGNNPVFTTDAPAAIGPYSQAIECRPSRMLFVSGQIPIDPATS
                     AIVPGGIEAETRQALRNIDAILTAGGMDRTNVVRATIFLASMSDFQVVNGIYAEFFGE
                     HKPARAAFQVAALPRGALVEIDAIAVE"
     gene            complement(657..1424)
                     /locus_tag="PKH54_08050"
     CDS             complement(657..1424)
                     /locus_tag="PKH54_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC99883.1"
                     /translation="MAEKLVALISGASAGIGAASARALAREGYGLLLGARRMDRLEQL
                     AAGIRAEFGVPVWCGTLDVRDGRSVAAFVDDGVAALGGIDVLVNNAGMARGLDRLDAV
                     APETWRQMLDTNVEGVIRLTQAALPHLRKSGRAHIVNISSIAGQGVYPGGGVYCATKH
                     ALDALTQTLRLELLAEPIRVSSIDPGMVETEFSIVRLGDADRAAQVYKDMTPLTAEDI
                     AECVRWVVTLPSHVNIDRITIKPREQGDLRTVCRRQQ"
     gene            complement(1442..3100)
                     /locus_tag="PKH54_08055"
     CDS             complement(1442..3100)
                     /locus_tag="PKH54_08055"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOC99884.1"
                     /translation="MKSIRMHLVQANPVVGDIDGNVALAMARWAQARAAGADLVIFSE
                     LFIVGYPPEDLVLKPVFLRRAMDAAHALALSCADGPGIVIGCPWEVEGRVFNAALLLE
                     GGSVKAASLKRQLPNYGVFDEMRVFCPGPVPSPVDFRGVRLGLPVCEDIWKPDVCREL
                     RRQGAEILVSINGSPFDVNKQGRRLEAASSRVRETGLPMVYVNQFGGQDELVFDGASF
                     VLGRDGDLRAGLRSWDEDGQDVVFLRDDGGWTCEPGRVEPRPGRLESIYMAMVVGLRD
                     YVVKNRFPGVLIGLSGGVDSALTAAVAVDALGSDKVHLVMMPYTFTSGVSLQEASATA
                     ALLGARYDVVPIAPAVEALSLMLSGLFAGRDPDITEENVQARVRGIVLMAISNKFGKM
                     VLTTGNKSESSVGYATIYGDMCGGYSVLKDIYKTDVFALSRWRNANFVSQFKGPEGRV
                     MPDRTIERPPSAELRPDQKDQDSLPPYAELDAILESLVEGEKSLSDIGAEGHAAETVC
                     RVENLLYQSEYKRRQAAPGVKISSRNFGRDRRYPVTNRFRDMNK"
     gene            3245..4234
                     /locus_tag="PKH54_08060"
     CDS             3245..4234
                     /locus_tag="PKH54_08060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOC99885.1"
                     /translation="MSHRVFVSRLIPEAGLEILRARTDLDVWSGEMPPSPTELRDRVK
                     GCDGAICLLSDTIDAAFFKAAGPALKVVSLFAAGYNNVDMNAAREARVIITNTPDQLT
                     EATAELAFTLMAAAARRVKEGIDNVRDGQWRTWGPLILRGRDLRGSTLGIIGMGRIGA
                     ALARRAVAFGMQVVFFDPSPFMIPPGDAPVTRVATLDELLGRSDFVSLHCPLTPQTRH
                     IIRKETIALMKPGCVLVNTARGPCIDTDALVDALKKDRIFAAGLDVTDPEPLPADHPL
                     VSLPNCLILPHVGSATFSTRDAMAVGAARNLLDVLDNRLPADTLNPDVFSLPH"
     gene            4314..>4860
                     /locus_tag="PKH54_08065"
     CDS             4314..>4860
                     /locus_tag="PKH54_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99886.1"
                     /translation="MKNLICAAIALSLLLPAVASARPGDTGFVVTSRKYNERENYAAI
                     CHEEFGIHARVADFADIRDLIRRDPYMRDFIEDTGLQAFKSSALLHFRGQKWGPRGEP
                     YYIQKRDHKRSRRTEGIIDQIGDGLFELKVRKNNAKPIMCFIPHEHRREAPRVRLPLT
                     PPPPPVLREPPPPPPPPPPPAR"
BASE COUNT          982 a         1589 c         1400 g          889 t
ORIGIN      
        1 ggctggtggt cgaaatcggc caggcaggca ccgccgaacg cttcgatctg ccggccggca
       61 aatcgatgcg ggttaccgcg gaaacggttc acaggttcat gtcgcccgac gacgaggcgg
      121 tcgaaatttt cgagacctcg accccggagc ttgacgacgt ggttcggctt gaagacgttt
      181 acggacgcga ggggacttca cgtccctgat ttgaatccag aatcaaggag ttccagatga
      241 agggcaacaa tccggttttc acgacggacg cgccggccgc gatcgggcca tactcccagg
      301 cgattgagtg caggccttcc aggatgcttt tcgtttcggg ccagatacca atcgacccgg
      361 cgaccagcgc gatcgtgccc ggcgggattg aggcggagac ccgccaggcg ttgcgcaaca
      421 tcgacgccat acttaccgcc ggcggcatgg acaggacgaa tgtcgtccgc gcgaccattt
      481 ttctggcgag catgtccgac tttcaggtcg tcaacggcat ttacgcggaa ttcttcgggg
      541 aacacaaacc ggcgcgggcc gcgttccagg tcgccgcact gcccaggggc gcccttgttg
      601 aaatcgacgc gattgccgtc gaatgaagcc tggcgccgtc ggcctgattg accgcactac
      661 tgctgtcttc tgcaaaccgt ccgcaggtcg ccctgttcgc gcggtttgat cgttatgcga
      721 tcgatattaa cgtgcgaagg aagcgttacc acccagcgga cgcactcggc gatgtcttcg
      781 gccgtcagcg gggtcatgtc cttataaacc tgggcagccc tgtcggcatc cccgagcctg
      841 acgatcgaaa actcggtttc gaccattcca ggatcaattg aagaaaccct tatcggctcg
      901 gcaagcagtt caagccgcag cgtctgcgtc aacgcgtcca gcgcgtgctt ggtcgcgcaa
      961 tagaccccgc cgccgggata gacgccctga cccgcgatgc tgctgatgtt gacgatgtgc
     1021 gcgcgccccg acttccgcag atggggcagc gcggcctggg tcagccggat caccccctcg
     1081 acgtttgtat cgagcatctg ccgccaggtt tcgggcgcca ccgcgtccag gcgatcaagc
     1141 ccacgcgcca tgccggcatt gttgacaagc acatcgatgc cgcccagcgc cgcaacgccg
     1201 tcgtcgacaa atgcggcaac cgaccggcca tcccttacat ccagggtgcc gcaccagacc
     1261 ggaacaccga attcggccct gattccggcg gccaactgtt cgagcctgtc catgcgccgc
     1321 gcgccaagaa gaaggccata cccttcccgg gcaagcgccc gtgccgatgc ggcaccgatt
     1381 cccgcactcg ccccggaaat aagcgcaacc agcttttcag ccatcttttc acctgcatca
     1441 gttacttgtt catatcccgg aaacggttag tgaccggata ccttctgtca cgcccgaaat
     1501 tccttgacga aatcttgacc cccggggcgg cctggcgtct cttgtattca gactggtaca
     1561 gcagattttc cacccggcac accgtctcgg ccgcatggcc ttccgcaccg atgtccgaaa
     1621 gggacttctc gccctcaacc aggctttcaa ggattgcgtc cagttccgcg tacgggggca
     1681 gcgaatcctg atccttctga tccggccgca attccgcgct tggcggccgt tcaatcgtgc
     1741 gatccggcat aacgcgccct tccggcccct tgaactgact gacgaaattg gcgttccgcc
     1801 accgcgacag ggcaaacaca tcggtcttgt aaatgtcctt caggacggaa tagcccccgc
     1861 acatgtcgcc ataaatggtg gcgtagccga cggaagattc ggacttgttt cccgtcgtaa
     1921 gcaccatctt tccaaatttg ttggaaatcg ccatcaggac tattccgcgc accctggcct
     1981 gaacgttttc ctcggtgatg tccgggtcac gccccgcgaa aagaccggaa agcatcaagg
     2041 acaaagcctc gaccgcaggg gcgatcggaa cgacgtcata cctggcgccc agcagggccg
     2101 ccgtggcgga ggcttcctga aggctgaccc cgctggtgaa ggtgtatggc atcatcacca
     2161 gatgaacctt gtccgaacca agcgcatcga ccgcgactgc cgcggtcagc gcgctgtcaa
     2221 cgccacccga aagaccaatc agcacgccgg ggaagcggtt cttgacgacg tagtcgcgca
     2281 ggcccacgac catcgccata tatatcgatt caagcctgcc tggacgaggt tcgacgcggc
     2341 ccggttcaca tgtccacccg ccgtcgtcac gaagaaagac gacgtcctgg ccgtcctcgt
     2401 cccatgaacg aagccccgcc cgcaggtcac cgtcgcgacc aagcacgaac gaagccccgt
     2461 cgaagacaag ctcgtcctga ccgccgaact ggttgacata aaccatcgga agcccggtct
     2521 cgcgcaccct tgacgacgcg gcttcaagtc gccggccctg cttgttgacg tcgaagggag
     2581 aaccgttgat ggaaaccagg atctcggccc cctggcgccg gagttcccgg catacgtccg
     2641 gcttccagat gtcctcgcag accgggagcc caagcctgac gccacggaaa tcgaccggcg
     2701 acggcaccgg cccggggcag aagacgcgca tctcgtcaaa aaccccgtaa ttcggcagtt
     2761 gacgcttcag gctggccgcc ttgacgcttc cgccttcaag cagcagcgcc gcgttgaata
     2821 cccggccttc gacttcccac ggacagccga tcacgattcc gggcccgtcc gcgcacgaca
     2881 aggccaacgc gtgtgccgca tccatcgccc gacgcaggaa gaccggcttc agaaccagat
     2941 cctccggtgg ataaccgacg ataaagagct cggaaaaaat caccagatcg gcaccggccg
     3001 cgcgggcctg ggcccatcgg gccatcgcaa gcgcgacatt cccgtcgata tcacccacca
     3061 ccgggttggc ctgaaccaga tgcatgcgaa tcgatttcat caacgcctcc ggacctgaca
     3121 tccggcggat gccggcggcc acaagttacc agcacggcga ccatttttcc agagggtgga
     3181 gcagcgccgc gaaatccggg ctatcatttc acgggccgtg tattccttca ggacggaggt
     3241 acagatgtca cacagggttt tcgtttccag gctgatcccc gaagcgggac ttgaaattct
     3301 gcgcgcacga accgatctcg acgtgtggag tggggaaatg cctccgtcgc caaccgaact
     3361 gcgcgatcgc gttaagggat gcgacggcgc gatatgcctg ctttccgaca ccatcgatgc
     3421 cgcctttttc aaagccgcgg gaccggcgtt gaaggtggtc agcctgttcg cggccgggta
     3481 caacaatgtt gacatgaacg cggcccgcga ggcgcgcgtg atcataacca acaccccgga
     3541 tcagttgacc gaagccaccg ctgaactggc cttcacgctt atggccgccg ccgccaggcg
     3601 cgtaaaggaa ggcatcgaca acgtacggga cggccagtgg agaacctggg gacctttgat
     3661 actgcggggc cgggatctgc gcggttcgac gctcggcatc atcgggatgg ggcgcatcgg
     3721 cgccgctctg gcccggcggg cagtcgcatt cggcatgcaa gtcgtcttct tcgacccgtc
     3781 gccgttcatg attccgccag gcgacgcacc ggtgacaagg gtagccacgc ttgacgaact
     3841 gctgggacga tccgacttcg tgtccctgca ctgcccgctg acgccacaaa ccaggcacat
     3901 aatccgcaag gaaacgatcg cattgatgaa gccggggtgc gtccttgtta acacggctcg
     3961 cggcccgtgc atcgacacgg acgcgctggt cgatgccctg aagaaagacc ggattttcgc
     4021 ggcaggcctg gacgtgaccg atccggaacc gcttccggca gaccacccgc tggtatccct
     4081 gcccaactgc ctgatccttc cccacgtcgg aagcgcgaca ttctcgacgc gggatgccat
     4141 ggccgtcggg gcagcacgga acctgctgga cgtacttgat aacaggctgc cggcggacac
     4201 tttgaacccc gacgtctttt cacttcctca ttgaggatct tcattgacga tcttccagcg
     4261 gccggctttt gtggtaacat tttttgagac ttttttacgg acggggaacc catatgaaaa
     4321 accttatctg tgccgcgatc gcgctttcac tgctgcttcc ggcagtcgca tccgcgaggc
     4381 cgggcgacac cggtttcgtc gtgacttcaa ggaagtacaa cgaaagggaa aactatgcgg
     4441 caatctgcca cgaggaattc gggattcacg caagggtcgc cgattttgca gacatcaggg
     4501 atctgattcg acgtgatccc tacatgcggg atttcatcga ggacaccggg ctccaggcgt
     4561 tcaagtcgtc ggccctgctt catttccgtg gtcagaaatg gggtccccgc ggcgagccgt
     4621 attacatcca gaagcgcgac cacaagcgtt cacggcgaac cgagggcatc atcgaccaga
     4681 tcggtgacgg ccttttcgaa ttgaaggtgc gcaagaacaa cgccaagccg atcatgtgct
     4741 tcattccgca cgaacatcgt cgcgaggctc ccagggtaag gctgccgctg acaccgcccc
     4801 cgccgccggt tctgcgtgaa ccgccgccgc cgcccccgcc gcccccgccg ccggcaaggg
//
LOCUS       DAOPPA010000235         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19166_length_4834_cov_3.045407, whole genome shotgun sequence.
ACCESSION   DAOPPA010000235 DAOPPA010000000
VERSION     DAOPPA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19166_length_4834_cov_3.045407"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKH54_08070"
     CDS             complement(<1..946)
                     /locus_tag="PKH54_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC99887.1"
                     /translation="MSVKVTSGVLAGIDGLMVDVEVDMHGGVGHFDIVGLPEASVRES
                     RVRVTSALRQLGHRITSRWITVNLAPADLRKTGSLYDLPIAVGVLACMDEIPTRSTDG
                     KMFVGELSLDGELRPVPGVLPMAAAAIKAGIGEILVPVENAREAAVVSGIRTVAIRDL
                     TSLIRYLAGDLSIEPTVSSPPDTTHIQMPDLADVRGQAHVKRALEIAAAGRHNLLMIG
                     PPGCGKTMLARRIVSILPPMTYGESLETSRIYSVAGLLRHRGGLVTDRPFRTPHHRAS
                     SASIVGGGPNPRPGEVSLAHNGVLFMDELPEWKREVLEV"
     gene            1316..>4834
                     /locus_tag="PKH54_08075"
     CDS             1316..>4834
                     /locus_tag="PKH54_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99888.1"
                     /translation="MGVRFSRLARAIGVGLYILVTRTLMTVAVTFTVLFFVLNSSPFP
                     DLIAKALVGVIPGSFEFGRIQVSPVPWIVDVTDVAIKTPGNKAIITSDLVRARIRLVP
                     LVESLLNLDRRKLLLHFASVRLDGFNVILQFDNDYHLELVDAFHIPDGRPPNPDPFVV
                     ALQFDHIEGTNGHAFVEFPDWDIRIDGIDMKTDFSLVSSPIGILVRARYFNWMSGIAH
                     VRAAPELNQIPRTVVLRQGFIDEFVYDWDEIAFSRFRAGFDFGSLDASDGFLSWGRNL
                     KYELAARLDLPEGSHVIRTATGGSVSGPLSVALSGSGDRGDPRFEIGVTSPALKVGPV
                     DLGQFDLSMTGGRDGLGFYGIRDIEASARSAGLAVTLSQGEFIPDTGTDKEAWNASAV
                     LDFAGVDISGLLRLADISVPSAAVPVPGVVEGSLRVGASGTQPGVSEGDGIPATVGSQ
                     TVVKASGKVSGSLSRKGVLDGRTFALDIDGDASWRDISHPVISFSRLRLKSGIDQVAV
                     SGMVDLASEKLVVSGLVTKELSSLTPLVGARMEGVVDVTALKLRGRISAPSGSARVAV
                     SDFAFDRWKVFQASAAVSLSGGILSVTDLKAATPFGQFMVDAARMPMTFRPGQIARGS
                     LDLSDIVVSGLDLQRFPLLAGTGISGFGHVRARSLSFGFRDILGTMTSMFSADIDWLL
                     VAGRRLETVSIQGSMDTGVISGLAVDAAVSGGGNISVSGRWDLPSERLDIDGSVSGVP
                     LAILAGLGPEAPVSGRVSADVNVSGVLDDPDIKGSARLEDLTFRGSRLAPVRLEADRS
                     QGDDLFLKSDKLFGGMKLGSGSRIVWKDGGFRSMRLEIDIRNLTPQDLLPNLAPRRLS
                     GSLTARLMIEIPSFSDPVISGRLESPPDGLWLEFFNRQTRIDNRGPLNISMEPDGSLV
                     VSGLQLDDGQSEIEVCGTANDSGINFLVRGSVGMHWLRALRGVFSSADGLVQFTGRER
                     FVPLLPGGCTSAMLKDGGALQISGSPKNPVVDGTVRTGQLTLNVRSLGDPIRIEPGGE
                     IELSRSSRGGRPVTGVLIPDRHWISGIFGEGVFNLSGRAVFDSGLLDDGLLRIKGSQI
                     RYVSPGEFFIVAEPRLQVDFKSLRLFGTPAAQEEPSVGVSGQVDISDGAYHRNFNVVR
                     SAFSGLTGGRVAERSGRSIFETVPWLG"
BASE COUNT          865 a         1318 c         1516 g         1135 t
ORIGIN      
        1 ggacttcaag gacttcgcgc ttccattccg gcaactcgtc catgaacagc actccgttgt
       61 gggcaagcga tacttccccc ggccttgggt tcgggccgcc gccaacgatg gacgcactcg
      121 aggctcgatg gtgcggtgtc ctgaacggcc gatcagtcac aagccccccg cggtgacgta
      181 gaagaccggc cacgctgtat atccggcttg tttccaggct ttcgccgtat gtcatgggtg
      241 gaaggattga cacgatccgt ctggcgagca tcgtcttgcc gcaacccggc ggaccaatca
      301 tcagcaggtt gtggcgtcct gccgccgcaa tttcaagtgc gcgcttgacg tgcgcctgtc
      361 cccttacgtc cgccagatcc ggcatctgga tgtgtgtcgt atccggcgga cttgatacgg
      421 ttggttcaat cgacagatcg ccagcaagat atcggataag ggaagtcaaa tcgcgaatcg
      481 cgacggttcg gatgccgcta accaccgccg cttcccgtgc attttcaaca ggcacgagta
      541 tttcgccgat tcctgccttg attgcggccg cggccatcgg caggacgccg ggaaccggcc
      601 gaagttcgcc gtcaagcgaa agctccccga cgaacatctt tccatccgtg gatcgtgtcg
      661 ggatttcgtc catgcacgca agcacgccga ccgcgatcgg caggtcgtac agggaaccgg
      721 tcttgcgaag atccgcgggg gccaggttga ctgtgatcca gcgacttgta atcctgtgtc
      781 caagttgccg gagcgcggat gttacgcgca cccggctttc cctgaccgac gcctcgggaa
      841 ggccgacgat gtcgaagtga ccaacgccgc catgcatatc gacttcgaca tcaaccatca
      901 gtccgtcaat gccggcaagc acgcctgaag tgactttcac gctcattgcg acgtccccgc
      961 gttttcagga ggccggattt tcgcaatgtg cgtgccatcg tcataacatt ccggatttgc
     1021 tggataagtg catcaatgcc gctgccgtga tattggacat tctcgcggtg cttgccctcc
     1081 cgtggtcagg tgagtccaac ttccgctcag ccggccttcc gcgcccgcgc aggcggcgcg
     1141 tacatgctcg cgtatgtgca ggcactgacg taaactgtgt aatattcacc ggctctggta
     1201 aattgtcgta ctgcgcgggt ccaggcgccc gggttccggt caatggctgg ctggacgcgg
     1261 ccccgcattg atgaacaggc cggcttggcc gacgcggcgg ccggcagggg cgcagatggg
     1321 cgtaagattc agcaggctgg ccagggccat tggagtcggc ctgtacatcc tggttaccag
     1381 gacgctgatg acggttgcgg tgacttttac cgtcctgttc ttcgtcctga attccagtcc
     1441 ctttcccgac cttatcgcca aggcgctggt cggtgtaatc ccaggcagtt ttgagtttgg
     1501 tcgcatccag gtcagtccgg ttccatggat tgtcgatgtt accgatgtgg cgataaagac
     1561 gccgggtaac aaggcaataa tcacctccga ccttgtgcgg gcacggattc ggctggttcc
     1621 gctggtcgaa tcactgttga atctggatcg acgaaagctg ttgctgcact tcgcttccgt
     1681 aaggcttgac ggtttcaacg ttattttgca gtttgacaat gactatcatc ttgaacttgt
     1741 tgacgctttc cacattccgg acggccgtcc cccgaacccg gatccgtttg ttgtagcgct
     1801 gcagtttgat catatcgagg gtacaaacgg tcatgccttt gtcgaattcc ccgattggga
     1861 catcagaatt gacgggatcg acatgaagac cgacttcagt cttgtttcct cgccgatcgg
     1921 gattcttgtc cgggcgagat atttcaactg gatgtcaggt atcgcccatg tcagggcagc
     1981 ccccgaattg aatcagatac cgcggacggt tgtattgcgc caggggttta tcgacgaatt
     2041 cgtttatgac tgggatgaaa tcgccttttc tcgcttcagg gcgggctttg atttcggttc
     2101 actggatgcg tcggatgggt tcctgtcgtg gggccggaat ctgaaatatg aacttgcggc
     2161 ccggttggat cttccggaag gcagtcacgt gatccggacg gccacaggcg ggtccgtaag
     2221 cggcccgctt tcggtggcgt tgagcgggtc tggcgatcgt ggggatcccc ggttcgaaat
     2281 cggcgtgacg tcgccggcgt tgaaggtcgg gccggttgat cttggccagt tcgatctttc
     2341 gatgactggc ggaagggacg gcctgggatt ttacgggatc agggatatcg aggcttccgc
     2401 acggtcggcc gggctggcgg tcactctttc gcagggtgag ttcatcccgg acaccggcac
     2461 cgacaaggag gcctggaacg cgtccgccgt actcgatttt gccggcgttg acatatccgg
     2521 actcctcagg ctggccgaca tcagcgtgcc ttcggctgcg gttcccgttc ccggcgttgt
     2581 tgaaggaagc ctgagggtcg gtgcttccgg aacgcaaccg ggggtgtccg agggtgacgg
     2641 gattccggcc accgtcggct cccagaccgt ggtcaaggca tcaggcaagg tgtctggaag
     2701 cctttcgcgg aagggtgttc ttgacggcag gacgtttgcc ctggacattg atggggacgc
     2761 ctcctggcgg gacatttcac atccagtcat ttcattctca cgcctgcgcc tgaagtcggg
     2821 catcgatcag gttgccgttt ccggcatggt cgatctggcg tccgaaaagc tggtcgtttc
     2881 gggtttggtc acgaaggaac tttcctcgtt gacacctctg gttggagcac gcatggaagg
     2941 cgtggtcgat gtgacggccc tgaaattgcg cggcaggata tcggcaccga gcgggtctgc
     3001 acgggtcgcc gtttcggatt ttgcctttga caggtggaag gtcttccagg catcggcggc
     3061 tgtttcgctt tcgggtggaa tcctgtctgt gacggatctt aaggcggcaa ctccattcgg
     3121 acagttcatg gtcgatgcgg ccaggatgcc gatgactttc agacctggac agatagccag
     3181 gggtagtctg gatctttccg acattgtggt aagtggactt gatcttcaaa ggtttccgct
     3241 tcttgcgggc accggtatat ctggattcgg acatgtgcgg gcacgatccc tgtcgttcgg
     3301 tttcagggac atccttggca ccatgacttc gatgttttct gccgatatcg attggctgct
     3361 cgtggccggc cggcgcctgg aaaccgtttc gattcagggt tcgatggaca ccggggtcat
     3421 ttccggactg gcggttgacg cggctgtaag tggcggtgga aacataagtg tttcgggaag
     3481 atgggatctt ccgagcgaac gcctggacat tgacggatcc gtaagtggcg tgccccttgc
     3541 gatccttgcc ggccttggtc cggaagctcc ggtatctggc cgggtatcag cggatgtgaa
     3601 cgtatccggg gttcttgacg atcctgacat caagggaagc gcgaggcttg aggacctgac
     3661 tttccgggga agtcggctgg ctccggtcag gctggaggcg gacaggtcac agggggacga
     3721 tctgttcctg aagtcggaca agctgttcgg cggaatgaag cttggatcgg gatcaaggat
     3781 tgtttggaag gatggtggct tccggtcgat gagactggag atcgacatca ggaacctgac
     3841 accccaggat ctgcttccca acctcgcacc gcgaagattg tccggaagtc tgacggccag
     3901 gctcatgatt gagatcccat cgttttcaga tccggttatc tcgggtcgac ttgagtcgcc
     3961 accggatggt ctgtggcttg agttcttcaa ccggcagacc cgcatcgaca accgaggtcc
     4021 gctcaacata agcatggaac ctgacggcag cctggtcgtt tccgggctgc agcttgacga
     4081 cggacagtcc gagatcgagg tttgcggcac ggcgaacgat tcaggaatca atttcctggt
     4141 tcgtggaagt gtcggaatgc actggcttcg agcattaaga ggtgtctttt cttctgccga
     4201 cgggcttgtc cagttcaccg ggcgagaacg gttcgtgccg ctgcttcccg gcggctgcac
     4261 ctcggcgatg ctcaaggatg gcggcgcact tcagatatct ggttcgccaa agaatccggt
     4321 ggtcgatggg accgtcagga ctggccagtt gaccttgaac gtcagaagtc ttggtgatcc
     4381 tatcaggatt gaaccgggcg gcgaaatcga gctttccagg agcagtcgcg gcggccgtcc
     4441 ggtaaccggg gttctgatac cggatcggca ctggatatcg ggaatcttcg gcgagggcgt
     4501 gttcaacctt tccgggaggg ccgtgttcga ttccggtctg ctggatgacg gtcttctgcg
     4561 tatcaaggga tcccagattc ggtacgtgtc gccaggcgag tttttcattg ttgccgaacc
     4621 aaggcttcag gtcgatttca agtcgcttcg gttgttcggc acccctgccg cgcaggaaga
     4681 accatctgtc ggggtatccg gtcaggttga tatctcggac ggcgcgtacc accggaattt
     4741 caacgttgtt cggtcggctt tttccggtct tacaggcggc cgcgtggcgg aaaggtccgg
     4801 ccggtcgata ttcgagaccg tgccgtggct tggc
//
LOCUS       DAOPPA010000236         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19272_length_4816_cov_4.298887, whole genome shotgun sequence.
ACCESSION   DAOPPA010000236 DAOPPA010000000
VERSION     DAOPPA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19272_length_4816_cov_4.298887"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1421
                     /locus_tag="PKH54_08080"
     CDS             132..1421
                     /locus_tag="PKH54_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99889.1"
                     /translation="MRPEDVRMVYCDEHGELFDHPELAMAVFDGMGFRLPYEDELVPL
                     PPGSDVYSMPGHSPVGAVPGTLDMEALSGFGAVAAFASPAWLRLNHPATDPDPGADAL
                     PLFAYAPLGWYRGRFYTTALRVDRSNRQDPRRFDLEAIGRAADALVGSHPSNRLIEHL
                     RHCAMVYGCRAAQNFFLGREEGPLPTSPSCNARCAGCLSKDPAGNVRASHDRISFVPS
                     PREIAETALIHIGRVRNAVVSFGQGCEGEPLMVADAIEGGIRLIRQATGRGTININTN
                     GSRPDAVGRLVDAGLDAIRLSINSFRPECYDAYYRQSGYTIDDVLESGRIVRSAGGFV
                     SVNYLVFPGVTDTKEEIAAALEGLAACRADFIQMRNLNIDPYLYLDTVGAPVSEPVGL
                     VKVMEAIRQRFPAIRFGYFNPPVRSLVQSRRRQRFSD"
     gene            1517..2731
                     /locus_tag="PKH54_08085"
     CDS             1517..2731
                     /locus_tag="PKH54_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC99890.1"
                     /translation="MRISAVADAIAGPATLKLNEKANQLKADGKPVIHLGSGEPKAPV
                     PQAATDAARVKLDSAVVRYSPTSGSPDLKKEVVEYTRRNYRYECSPANVLISSGAKPA
                     IFFALYSVVQPGDEVVFPAPYWVSYPEMARMLGGVPVVVAPEPGTFEPSFESFAAAVT
                     DRTRAMIINSPNNPSGHVYSAALIKDLVRLCEERDICLIMDDIYHKLVFDGREAPSAF
                     DFARRTGEDSVIVAVNGVSKLYAMTGFRIGWAVASKRMINVMKNVQAQILSCPSSLAE
                     AAALGALEGDQGCVESLRSTLQANRDVMVGELSKIPGVKLNAPGGTFYSLPDFSAIDA
                     NSHRLCEFLVEKVMVVTVPGKDFGAEGHLRFSYCGSAREIIEGVERVRWALDPNGPRQ
                     ITIGSTTFTRDW"
     gene            2789..>4816
                     /gene="priA"
                     /locus_tag="PKH54_08090"
     CDS             2789..>4816
                     /gene="priA"
                     /locus_tag="PKH54_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOC99891.1"
                     /translation="MSEENLPIADVLLLLPLARTYSYTVPPRLAGVVTRGTQVLCPVR
                     RSRVHGIVLDVRGPRDDDPELLEIIDVSERPAWPADLMELMGWVSTYYVAPPGTVART
                     AMPGLFARRKAAVDEYVVFVRCPDSPGRSQRVLKVLDVMERCGSMEIGQARKIVPGGS
                     DVVRKLLAGGYLRVENRTAAEFMPPSCMDDGDRPTLTRDQQSVLDAIPTEKGRYSPFL
                     LHGVTGSGKTEVYMRAIERVLAEGRGAIVLVPEIALTFQLRTTFERRFKDAAAIMHSQ
                     MSDSVRAAAWDEILAGRRRLVVGARSAIFAPVRDLGLIVVDEEHDTSYKQQDGLRYNA
                     RDLALVRGKIAGCTVLLGSATPSLETFNNTANGKVGYLSLPSRVRELPMPCVEFANMK
                     YAQTRGDEKLFSEQLAVELNRTIDAGESAILFLNRRGFGRFVMCRTCGRKIDCPDCSI
                     TLTHHARPDRLVCHYCDHHQALPGLCPSCGSGELGIMGFGTERLEEETQRLFPKARVC
                     RLDSDTAGRTKEILSDFRDGRFNVLVGTQIVAKGHDFPAVTLVGVMLADQSLAFPDFR
                     ASERTFQLLTQVSGRAGRGSVPGRVIIQTYCPENYALRYSSTHDYLGFAVEENKLRRE
                     RGYPPYSFLASIEVSSTDAVAASSTASGIVEFIIGQIGALGGPPGQIRVLGP"
BASE COUNT          892 a         1381 c         1546 g          997 t
ORIGIN      
        1 atgcttgtga caattccttc accaggcggg tttgaatccc tgaatgtggg cgtcactgcg
       61 gggataatgc tttgtgccgc cagcggccag gtcccccagg cttcaaggcc gccggaacca
      121 tcggaggact gatgcgacct gaagatgtca ggatggttta ttgcgacgaa cacggagaac
      181 tgttcgacca ccccgaactg gcgatggccg tgtttgatgg aatggggttc aggcttccat
      241 acgaggatga actggtgccc ctgccgcctg gcagcgatgt ctacagcatg ccgggccaca
      301 gtccggtcgg agcggttccc ggcacgctgg acatggaggc tctttccggt ttcggtgcgg
      361 tggccgcctt tgcgagtccc gcctggctgc ggttgaatca tccggcgacc gatccggatc
      421 ccggggcgga cgcactgcca cttttcgcgt atgcacccct gggatggtac cgtggtcgct
      481 tctatacgac cgccctgcgc gtcgatcggt cgaatcgcca ggatccacgc cggttcgacc
      541 tggaagctat cggccgggcc gccgacgcgc ttgtcggcag tcatccatcg aacaggttga
      601 tcgagcatct gcggcattgc gccatggttt atggatgccg cgcggcgcag aatttcttcc
      661 ttgggcgcga ggaagggccg cttccgacat ccccttcgtg caacgcccgc tgcgcgggct
      721 gcctgtcgaa ggatcccgcc ggaaatgtcc gcgccagcca cgacaggatc agtttcgtgc
      781 cgagtccccg ggagatcgcc gagacggctt tgattcacat aggccgggtc aggaacgccg
      841 tcgtgagctt cgggcagggt tgcgaaggcg aaccgctgat ggtcgcggac gcgatcgagg
      901 gcgggattcg gttgatcagg caggccaccg ggcgcggaac gatcaatatc aatacaaacg
      961 gcagcaggcc cgatgcggtg ggcagactgg tggatgccgg cctggacgcc ataaggctct
     1021 cgatcaacag cttcaggccg gagtgttacg acgcctacta cagacagtcg ggatacacga
     1081 ttgacgacgt ccttgaaagc gggcgcatcg taaggtccgc gggcggtttc gtgtcggtca
     1141 actacctggt ctttcccggc gtcacggaca cgaaggaaga gattgccgcc gctctcgaag
     1201 ggctggccgc ctgtcgtgcc gacttcatcc agatgcgcaa cctgaacatc gatccatatc
     1261 tgtacctgga caccgttggc gcccccgttt cggagccggt cgggcttgtg aaggtcatgg
     1321 aggcgatacg gcaaaggttt ccagcgatca ggttcggata tttcaacccg ccggtccgga
     1381 gtcttgtgca gtcccgccga aggcaacgct tcagcgactg acattgttta ttttctttga
     1441 cttataccga cggcgataga caatgggcac ggcccgggct gctgtctgtc cgggaaggtc
     1501 ttcatggaga ttgaacatga gaatcagtgc tgtggcggac gcgattgccg gaccggcaac
     1561 gctgaagctg aacgagaagg caaaccagtt gaaggcggac gggaagccgg tcatccatct
     1621 gggaagcggg gaaccgaaag cgccggttcc gcaggcggca acagatgccg ccagggtcaa
     1681 gcttgattcg gcggtcgtaa ggtattcgcc gacttcgggt tcaccggatc tcaagaagga
     1741 ggttgtggaa tacacccgcc gcaattaccg gtacgagtgt tcccccgcca acgtcctgat
     1801 atcctccggc gccaagccgg caatcttctt tgctctttac tcggtcgtcc agcccggcga
     1861 cgaggtcgtc tttcccgccc cttactgggt cagttatccc gaaatggcac ggatgctggg
     1921 tggcgtcccg gtagttgtcg cgccggagcc gggcacgttc gagccttcgt ttgagtcttt
     1981 cgccgcggcg gtcaccgacc gcaccagggc gatgatcatc aacagtccga acaacccgtc
     2041 gggacacgtc tattctgcgg cgttgataaa ggatctggtg cgcctgtgcg aagaacgcga
     2101 catctgcctg ataatggacg atatctatca caagctggtg tttgacggcc gtgaagcgcc
     2161 ctcggcgttt gatttcgcgc gcaggacggg cgaggactcg gtcatcgtag cggtcaacgg
     2221 cgtaagcaag ctttacgcca tgaccggctt ccgtatcggc tgggccgtcg cgtcgaagcg
     2281 gatgatcaat gtcatgaaga acgttcaggc gcagatcctg tcgtgtccgt cgtcgcttgc
     2341 cgaggcggcc gcccttggcg ccctcgaagg tgaccagggc tgcgtcgaat cgcttcgttc
     2401 gacccttcag gccaatcgcg atgtaatggt cggggaactg tcaaagattc ccggtgtgaa
     2461 attgaacgcg cctggcggaa ccttctacag tcttcccgat ttctcggcaa ttgacgcgaa
     2521 ttcacatcgt ctgtgcgagt tcctggtcga gaaggtgatg gtcgtgactg ttcctggaaa
     2581 ggattttggg gccgagggcc acctgaggtt ttcgtactgc ggttccgcca gggagattat
     2641 cgagggcgtg gaacgcgtcc gctgggcact tgatccgaac ggtccccgcc agatcacgat
     2701 cggttcgaca actttcacca gggactggtg acagccgcgt ccaggtagcg atacgtcgtc
     2761 acggttttgg ggtatgatcc cttccgccgt gagtgaagaa aacctcccga ttgccgatgt
     2821 gctcctgctg ctgccgctgg cgcgtacgta ctcctatacc gtgcctccca ggctggccgg
     2881 agtcgttact cgcggtaccc aggtactgtg tcccgtgcgg cgatccaggg ttcacggcat
     2941 cgtcctcgac gtccgcggcc cgagagacga cgaccccgaa cttcttgaaa tcatcgacgt
     3001 gtcggagagg cctgcctggc cggcggatct gatggaactg atgggatggg tatccacgta
     3061 ttacgttgcc cctccgggta ccgtggcaag aaccgccatg ccgggcttgt tcgcgcgccg
     3121 caaggcggcg gtcgatgagt acgtggtgtt cgtaaggtgt cccgattccc cggggcgatc
     3181 ccagcgtgtg ctgaaggttc ttgatgtcat ggaaaggtgc gggtcgatgg agatcggcca
     3241 ggccaggaag attgtcccgg ggggatccga tgtagtcagg aagctgcttg ccggcggtta
     3301 cctgcgggtc gagaacagaa ccgcggccga attcatgccg ccttcgtgca tggatgacgg
     3361 cgacaggccg actttgacca gggatcagca gtccgttctt gacgcgatcc cgaccgaaaa
     3421 gggcagatat agtccgtttc tgcttcacgg cgttaccggt tccggaaaga ccgaagtcta
     3481 tatgagggcg atcgagcgcg tccttgccga gggtcggggc gccatcgtgc tggttccgga
     3541 aattgccctt acctttcagt tgaggactac gttcgagcgg cgtttcaagg atgccgcggc
     3601 catcatgcac agccagatga gcgattcggt cagggcggcc gcctgggacg aaatcctggc
     3661 cgggcggcgt cgcctggtcg ttggggcaag gtcggcaatt ttcgcgccgg tcagggacct
     3721 cggcctgatt gtggtcgatg aggaacacga cacttcgtac aagcagcagg acggtctcag
     3781 gtacaacgcg cgcgacctgg cgctggtgcg gggaaagatt gccgggtgca ccgttctgct
     3841 cggatccgcc acgccttcgc tggagacttt caacaacacg gccaacggga aggtcggcta
     3901 tctgtcattg ccgtcccgcg tcagggaact tccaatgccg tgcgttgaat tcgcgaatat
     3961 gaagtacgcg cagacccgcg gcgacgagaa gcttttttcg gaacagctcg cggtcgaact
     4021 gaacaggaca atcgacgccg gagagtcggc gatacttttt cttaacaggc gcggattcgg
     4081 caggttcgtg atgtgcagga cgtgcggtcg caagattgac tgccccgatt gttcaatcac
     4141 cctgacccac catgcgcgtc cggatcgcct tgtatgtcat tactgcgacc accaccaggc
     4201 gttgccggga ttgtgtccgt cgtgcggatc cggtgaactc ggtatcatgg ggttcggaac
     4261 cgagcgcctg gaagaggaga cgcagaggct gtttccaaag gccagggtgt gcaggcttga
     4321 ctccgatacc gccggacgaa cgaaggagat actttccgat ttccgcgatg gccggttcaa
     4381 cgttctggtc ggaacccaga tcgttgccaa gggtcacgac tttccggcgg taacccttgt
     4441 cggcgtgatg ctggccgatc agtcgctggc tttcccggac ttcagggcat ccgaaaggac
     4501 gtttcagctt cttacgcagg tctcgggcag ggccgggcgc ggcagcgtgc cgggacgcgt
     4561 aatcattcag acctactgtc ccgagaacta cgcgctgcgg tattcatcga cccacgacta
     4621 tctgggtttt gccgtcgagg aaaacaagct gaggcgcgaa cgcgggtatc cgccgtactc
     4681 gttccttgcg tcgatcgagg tgtcatccac ggatgccgtc gcggcctctt caacggcgtc
     4741 cgggattgtc gaattcatta ttgggcagat aggggccctg ggcggccccc cgggacagat
     4801 ccgggtactt ggcccg
//
LOCUS       DAOPPA010000237         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19392_length_4793_cov_4.007809, whole genome shotgun sequence.
ACCESSION   DAOPPA010000237 DAOPPA010000000
VERSION     DAOPPA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19392_length_4793_cov_4.007809"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..923
                     /locus_tag="PKH54_08095"
     CDS             192..923
                     /locus_tag="PKH54_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99892.1"
                     /translation="MLVTRFEDSARFIGRLDAAQDVAAGFKTVCRENSIRCAWICATM
                     MLRNPVIAVAGHSGFGQEAIVDGTRFFPSVTGNVSLLGDEVHVRLYGQSEGTQSQQAA
                     TGLILGGEVLFCEFLITTCEDAALIRERVAPFSPWVQLQPSGNVVVPEFQPSPQRPTT
                     IQPAALTDDVEATELNILEMKAGDYVDHPRFGVCRISADPADDKISIKLATGKQVDLS
                     LGVMKVHDPKQLGGRKVFKLEIRRR"
     gene            complement(935..2323)
                     /gene="glmU"
                     /locus_tag="PKH54_08100"
     CDS             complement(935..2323)
                     /gene="glmU"
                     /locus_tag="PKH54_08100"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOC99893.1"
                     /translation="MDRLAAVVLAAGLGTRMKSEKAKCLHCAAGMPLIWYPVKAAFDA
                     GAEKVVIVVGHQREAVQAEVSRLFPERAIEFVVQERQLGTAHATVCAADSVAGYDLAV
                     FINGDLPLLTGRTVSGVVDAFRKSGAFALLTAHVRNPGGFGRIVRDDRGVSEIVEARD
                     ADPCQLAIDEVNVGVYVAAPGPLFEKLAQIRAASGSGEYYFTDVVKSFRADGRDVGSF
                     VLADSTDARQVNDRLELAVAEGLLHARKVRELMLAGVTVHQPDSQMIDFDCSIGCDTE
                     VFPGCELRSATRIGSRCVVGRGCVLSATSVGDGVEIKPYSVLFDSVVEDGCVLGPFCH
                     LRPASRAMQGSHVGNFVELKKTTLGPGSKANHLAYLGDCLIGSGANIGAGTITCNYDG
                     YKKMPTEIGDGAFIGSDSQLVAPVKVGAGAYVAAGTTVTADVPAGALAISRVEQKHVE
                     GYAARKKARSGR"
     gene            2470..3261
                     /gene="xth"
                     /locus_tag="PKH54_08105"
     CDS             2470..3261
                     /gene="xth"
                     /locus_tag="PKH54_08105"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOC99894.1"
                     /translation="MNGLAVAATWNVNSIRARREHLLDWLARERPDYLCLQELKAPDG
                     EFPVVDVEAAGYHVMAFGQKTYNGVAILSLNTPLSVEKGMGDDDPQARLLTCQFPDMT
                     VISAYFPNGSEPDSDKYQYKKEWLGRFTTWLGKRLESFGPTILCGDFNIAPTAQDVGN
                     ESEWEGSVIYNPQMRQAFSDLQALGLVDVFRSLNPLATDFSWWDYRMNAFRRNLGLRL
                     DYIMATANLAGAAVSARIDRAERAMEKPSDHAPVIASFDMSRVRR"
     gene            complement(3281..3787)
                     /locus_tag="PKH54_08110"
     CDS             complement(3281..3787)
                     /locus_tag="PKH54_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOC99895.1"
                     /translation="MGKTTDNLKAAFAGESQARNKYIAYGRKAELEGMPNVAKLFRAA
                     ADAEEVHAIRHWKALGMVRDTLQNLEDGLAGETYEIVDMYPEFLAQAEKEQEITATYA
                     FKGALEAEKIHQKLYAAAIEAVKAGARDIEAFRVYTCEVCGYTHVGEPIEKCPICSVP
                     WTKFHEVL"
     gene            4089..4700
                     /locus_tag="PKH54_08115"
     CDS             4089..4700
                     /locus_tag="PKH54_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC99896.1"
                     /translation="MITLRTFSFIDSLQPQLAAYLGKTAQGFLPLPEQASLFVEVQPA
                     IIINRITDRALKATTVAPGMQVVERAFGVVELHDFDKGEVERAGAAILDYLKLSENDR
                     MKPVITSTQIIRSIEPFQAQIINRNSWGMMILPGQSLFILETEPAGYVAIAANEALKA
                     ADVSLVEIHPHGAYGRLWLSGSESEIDSAAEAAIKVLKSMSGV"
BASE COUNT         1003 a         1466 c         1400 g          924 t
ORIGIN      
        1 atccgtcacg gaaggaagca accctgttga cgctgataga aggcgtcggc gaatccagga
       61 tcaaggcagt cttcaacggc gatgcgtggg ttttcgagtt catcgactcg atgccccagg
      121 cgcccgcgat gccaccggta ccaacaccct gacgccagtc atgcgccggg gacgaactcc
      181 aggggaaagt catgctggtt acaaggttcg aggactccgc aaggtttatc ggaaggcttg
      241 acgccgcgca ggatgtggcg gcagggttca agacggtctg ccgggaaaac tccatcaggt
      301 gcgcatggat ctgcgcgacc atgatgctcc gcaatccggt gatcgccgtc gcgggccatt
      361 cggggttcgg acaggaggcc atagttgatg gcacgcgatt cttcccgtcg gtcacgggca
      421 acgtttccct tcttggcgac gaggttcatg tgcgcctgta cggccagtcg gaagggactc
      481 agtcccagca ggcggcaacc gggctgatac ttgggggcga ggttcttttc tgcgaattcc
      541 tgattacgac gtgcgaggat gctgccctga tccgcgagcg ggtcgccccg ttctcgccat
      601 gggttcaact tcagccgtcg ggaaatgtcg tggttccgga gtttcagcca agtccccagc
      661 ggccaacgac gatacagccg gcggccctga cggacgatgt cgaggcgacc gaactcaaca
      721 tccttgaaat gaaggctggc gactatgtcg atcatcccag attcggcgtg tgccgcattt
      781 cagccgaccc tgccgacgac aaaatttcaa tcaagctggc cacgggaaag caggtcgatc
      841 tcagccttgg cgtgatgaag gtacacgacc cgaagcagct tggcggacgg aaggttttca
      901 aactcgaaat cagaagacgc tgaacgagat tccctcacct gccggaacgg gccttcttcc
      961 ttgcggcata tccttcgacg tgcttctgtt caacgcgcga aatagccagg gctccggcag
     1021 ggacatccgc cgtcaccgtc gtgccggcgg caacgtacgc gccagccccg accttgaccg
     1081 gcgcgacaag ctgggaatcg gatccgataa aggcgccgtc gccaatctcc gtcggcatct
     1141 ttttgtagcc gtcatagttg caggtaatcg tcccggcccc gatattggcg ccagaaccga
     1201 tcagacagtc cccgaggtac gcaaggtggt tggccttgga accgggcccc agtgtcgttt
     1261 tcttcagctc cacgaaattc ccgacgtgag agccctgcat tgccctggac gccggccgga
     1321 ggtggcagaa ggggccaagg acgcaaccgt cttcaacaac cgaatcaaaa agaaccgaat
     1381 acggcttgat ctcgacgccg tcgcccaccg atgtcgccga cagcacacac ccgcgtccga
     1441 cgacgcaccg ggaaccgatc ctggtggccg aacgcagttc gcatcccgga aagacttcgg
     1501 tgtcgcagcc aatcgagcag tcaaaatcga tcatctgcga atcgggctga tgaacagtca
     1561 cgccggcgag cataagttcg cgaaccttcc gggcgtgaag cagaccctcg gcaacggcca
     1621 gttcaagcct gtcgttcacc tgcctggcgt ccgtcgagtc ggccagcaca aacgatccaa
     1681 catcgcgtcc atcggccctg aacgacttga cgacatccgt gaagtaatac tctcccgaac
     1741 cgcttgccgc ccgaatctgc gccagtttct caaacagagg accaggcgcg gcgacataga
     1801 cccccacgtt cacttcatca atcgcgagct ggcaaggatc cgcgtcacgg gcctcgacaa
     1861 tttccgatac gccgcgatcg tcccgaacaa tcctgccgaa tccaccggga ttccggacat
     1921 gcgccgtcag cagggcaaaa gcacccgact ttcggaaggc atccacaacg ccgctgacag
     1981 tccttccggt caaaagcggc agatcgccgt tgatgaatac ggccaagtcg taccccgcga
     2041 ccgaatcagc cgcacacacg gtggcatgcg cggttccaag ctgacgttcc tggacgacga
     2101 actcaatcgc ccgttcggga aacaaccgcg aaacctccgc ctgaaccgct tcacgctggt
     2161 gaccgacaac gatgacgacc ttctcggcgc ccgcatcgaa cgccgccttc accggatacc
     2221 agatcagggg catcccggcc gcgcaatgca ggcacttggc cttttccgat ttcatccggg
     2281 ttccgagccc ggccgcgagg acgacggccg caaggcgatc cattggcacc tccatgccgg
     2341 gagacttccc ggccatcaac gattccgggg gatgatatca cgcgttgcgc caagcgaggc
     2401 aatgcgcgac cagcgtgctc cgggattcgc gggacgtgtt atcctgcctc ggatatcagg
     2461 aggtcgccga tgaacggact tgcggtcgcg gcaacatgga acgtgaactc gatcagggca
     2521 aggcgcgagc acctgctgga ctggcttgcg cgcgaacgac ccgactatct gtgcctgcag
     2581 gagttgaagg cccccgacgg cgagtttccc gtcgtggatg tggaggccgc cggttaccat
     2641 gtcatggcat tcggacaaaa aacatacaac ggcgtggcca ttctttcact gaacaccccc
     2701 ttgtccgttg aaaagggtat gggcgatgac gatccccagg cacgcctcct gacgtgccaa
     2761 tttccggaca tgacagtcat ttcagcgtat ttcccgaatg gttccgaacc ggacagcgac
     2821 aagtatcagt acaagaagga atggcttggc cgcttcacga catggctcgg caagcgcctt
     2881 gagtcattcg gaccgacgat cctttgcggc gacttcaata tcgcgccgac ggcgcaggat
     2941 gtgggcaacg aatcggagtg ggaaggctcg gtgatataca acccgcagat gcggcaggcc
     3001 ttttcagacc tccaggcgct tgggctggtc gatgtcttcc gcagcctgaa cccgctggca
     3061 accgattttt cctggtggga ctacaggatg aacgcgtttc gaagaaacct gggactgcga
     3121 ctggactaca taatggccac cgccaacctc gccggggcgg ctgtctcggc cagaatcgac
     3181 cgtgcggaac gagccatgga aaagccgtcg gatcatgcgc cggtcatcgc gtcattcgac
     3241 atgagcaggg ttcgacgctg acgggaacgg tgcatcttcc ctacaggact tcgtggaact
     3301 tcgtccacgg aacagagcag atcggacact tctcgatcgg ttcaccaacg tgggtgtaac
     3361 cgcagacctc gcatgtatat acgcggaatg cctcgatgtc cctggcgcct gccttgaccg
     3421 cctcgatggc cgcggcatac agcttctggt ggatcttttc ggcctcgagg gcgcccttga
     3481 aggcataggt cgccgtgatt tcctgctcct tctcggcctg ggcgaggaat tctggataca
     3541 tatcgacgat ttcgtacgtt tcgccagcaa gcccgtcctc gagattctgc aacgtgtcgc
     3601 gtaccattcc aagggccttc cagtggcgga tggcgtggac ttcctcggca tctgctgcgg
     3661 cccggaagag ctttgccacg ttcggcatgc cttcgagttc ggccttgcgc ccatatgcga
     3721 tgtacttgtt cctggcctgg ctctcaccag caaaggccgc cttcaggttg tctgtcgtct
     3781 tgcccatgtc gatcctccat gaatccggtt gacttgaaaa acaacggaaa cgttgaccac
     3841 ggaatgttgc acgcagcggg gtgacaaggt caatagattt gatgtaaccc gttgaaccgc
     3901 cggccttcag gctcacgcat ggaaccgatc cagacgcatc ttgcctgaac atccgtacac
     3961 actgtttttc acgcaatagc tatcgactgg tggccgcttc ctgtgataat ctgccgcgag
     4021 tcttggccac gaccctggaa cggacggaag taccggcccg gccgggcgtg gcgacctgga
     4081 cgactgaaat gattaccctg cggacctttt cattcatcga cagcctgcag ccgcaactcg
     4141 cggcctatct gggcaagacg gcccagggat tcctgccgct gccggagcag gcctcgctgt
     4201 tcgtcgaggt ccagccggca atcatcatca acaggatcac ggatcgggca ctgaaggcaa
     4261 caacggtggc ccctggcatg caggtggtgg agcgggcctt cggcgtggtc gaactccacg
     4321 atttcgacaa gggcgaggtc gagcgtgccg gcgccgcgat cctggactac ctgaagcttt
     4381 ccgagaacga ccggatgaag ccggtgatca catcgaccca gattattcgt tccatcgaac
     4441 cgttccaggc ccagatcatc aacagaaatt cgtggggcat gatgatcctt cccgggcaga
     4501 gcctgttcat tcttgaaacc gaaccggcgg ggtatgtggc gatcgcggca aacgaggcac
     4561 tcaaggccgc cgacgtctcc ctcgtcgaaa ttcatcccca cggcgcttac ggacggctat
     4621 ggctctcggg atccgaatcc gaaatcgatt cggcagccga ggcggcgata aaggttttga
     4681 aatcgatgtc cggcgtttga tgccgggcga ctggatgtgc ccgcgcggac gcgacgggct
     4741 ttgttaagtt ctggaggtta acgatggctg atgctcttgg aatgatcgaa acg
//
LOCUS       DAOPPA010000238         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19513_length_4767_cov_4.314304, whole genome shotgun sequence.
ACCESSION   DAOPPA010000238 DAOPPA010000000
VERSION     DAOPPA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19513_length_4767_cov_4.314304"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKH54_08120"
     CDS             complement(<1..696)
                     /locus_tag="PKH54_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC99897.1"
                     /translation="MLIRRNQIKAAEFRELQWLDHDPMTLASLEGQVVLLYFWDYSYV
                     VSVRNLVYLSMWHGRYADKGLQIVAIHAPQFDFGKDERNLRQAVEYHGLPFPVANDVN
                     LRTWDAYANRFWPATFLIDRQGYLAACHFGEGGINEIEMSMQELLKEGRSRVVMPKDS
                     LVGQQDRELQRRPVSPSLYFGYRRGRIGNGNGFTPGEVCEYETPDRFRRDIHYMKGAF
                     RCLADSMILSGLQG"
     gene            complement(725..1252)
                     /locus_tag="PKH54_08125"
     CDS             complement(725..1252)
                     /locus_tag="PKH54_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99898.1"
                     /translation="MPEITMADKIGRTQFLGREFLTWLLFCAYSDQSVKVGDQVIGVA
                     FERSVTLDGENPAREMSAIKVDEPTQSSEVMLSLKLGKKVSKARLMLTVDGREFKTGI
                     ESSTLAMKSLKLPDVKGGDFADIFEERMDLTDLVEQAVWDLFTHFVRIRIDQEEWSRE
                     AERISRWIESEDIFG"
     gene            complement(1245..1937)
                     /gene="rdgC"
                     /locus_tag="PKH54_08130"
     CDS             complement(1245..1937)
                     /gene="rdgC"
                     /locus_tag="PKH54_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOC99899.1"
                     /translation="MGFNNQTVSYVTYRVTEDVNGEELRQSILRGLTVGRISPIDVDL
                     GHDQSFGFATFEDPLETEFTEQNIFFDPLAVFSFRVDKLAVPAGTLRIYTRKRLQDRL
                     AASSRSRMPREERDELSEQVRLELLRRALPSINAVDVIWDMARGIIRFYSLSQSLNEA
                     FLVRFNQFLDLKLQAMNPVGILESRLDEREIEQVWHLLPTSFLFGGTIEPGTATFDPE
                     PCADDEGEIENA"
     gene            complement(1956..2390)
                     /gene="tsaE"
                     /locus_tag="PKH54_08135"
     CDS             complement(1956..2390)
                     /gene="tsaE"
                     /locus_tag="PKH54_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOC99900.1"
                     /translation="MRILREYRTTSEEETEAAAAELLRMASPGEVIGLVGELGAGKTV
                     FVRGAVRALGGDPTQVHSPTFTIMNIYRARIPVHHFDLYRITDAGDLESTGYYEFTSS
                     GAVSLVEWADKIPDVAAQVDFMISIRLEPDRLTRVISVHEQD"
     gene            complement(2404..2883)
                     /gene="greA"
                     /locus_tag="PKH54_08140"
     CDS             complement(2404..2883)
                     /gene="greA"
                     /locus_tag="PKH54_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOC99901.1"
                     /translation="MDESRVPLTPEGFARLQEDLKRWKEEMPIIIKAIGEARDLGDLS
                     ENAEYHSARERQGLIDANIKHLESLIARAEVIDPSRFRGDKRIRFGATVTIFDEDRDE
                     EIVYRIVGDAEADIDRNMISVNAPLGRALIGKEDGDDVKFKTPRGERRLSIVAVEYV"
     gene            complement(2968..>4767)
                     /locus_tag="PKH54_08145"
     CDS             complement(2968..>4767)
                     /locus_tag="PKH54_08145"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOC99902.1"
                     /translation="FSNDQESFLNKVSQAGLDPGDRTVQSFIDVWHQMQDLPRHIGQH
                     NGGLVISGNRLDRVVPIVPARMAGRRVIQWDKDDIADMRMIKVDLLGLGMLAAISKTI
                     ELVSRHQGVDVDLAGIPVNDKDVWDMLCRADTIGVFQIESRAQMATLPRMRPRCFYDL
                     VIEVAIIRPGPIVGKMVHPFLERRCGAAPVRYAAPCLQPILERTLGIPLFQEQLMRMA
                     MTAAGFSGGESEELRRAMGSRRSRARMLRLVEKLHAGMERNGIEKPARDEIVSSIISF
                     ALYGFPESHAASFALIAWASAWLKFHHHAAFTASLLNCWPMGFYSPATLVRDAMHHNV
                     PVLPIDVNKSASDCTLEHAADGKIAIRLGLKFIRGLAASTIDRIIRKRPTGGYRDITH
                     VGEAALAGRSELMIIADAGAAPASGPGLLTEGRGTRRDALWQAMAFDPSATILHRNQS
                     DTDMPECVEPLLEHSFEDMANDFRATGLTTGPHPIAFIRPQLEVRGIKPADGLSLLPD
                     GAHASVAGMIIVKQRPPTAKGFYFATVEDESGHAAVAVSPAVFARNRTLLLTSSFLIF
                     SGVVQNRSGVTSLLADSFERLPKLVNHPGRDFH"
BASE COUNT          938 a         1433 c         1344 g         1052 t
ORIGIN      
        1 gccctggagc cccgaaagaa tcatgctgtc cgccaggcac ctgaaagcac ccttcatgta
       61 atgaatatcg cgcctgaacc tgtccggcgt ttcgtattcg catacttcgc ccggggtgaa
      121 cccgtttccg ttgccgattc gtccccggcg gtaaccgaaa tacagcgacg ggctgaccgg
      181 tcttcgctgc agttcccgat cctgctgacc gacaaggctg tccttcggca tgacaacgcg
      241 gctgcggcct tccttcagca gttcctgcat cgacatctcg atttcgttga tgccgccttc
      301 tccgaaatga cacgcagcca ggtatccctg gcggtcgatg aggaatgttg ccggccagaa
      361 gcggttggca taggcgtccc aagtcctcag gttgacgtca ttggcgaccg gaaacggcag
      421 accatggtat tccaccgcct gacggaggtt gcgctcatcc tttccgaaat cgaactgggg
      481 tgcgtggatc gcgacgatct gcaatccctt gtcggcgtac cggccgtgcc acatgctcag
      541 atacaccagg tttcttaccg ataccacgta ggaataatcc cagaagtaca gcagcacgac
      601 ctgcccctcg agagaggcaa gcgtcatcgg atcatgatcg agccattgca gttccctgaa
      661 ttcggcagcc ttgatctggt tgcgcctgat gagcatcggt catccgccct ttttgaaaag
      721 cctgtcagcc gaatatgtct tcgctttcaa tccagcggga aattcgttcg gcttcgcgtg
      781 accattcttc ctgatcaatc cgaatcctga cgaaatgggt gaagagatcc cagacagcct
      841 gttcaaccag gtcggtcaga tccattcttt cctcgaagat gtcggcgaag tcgccaccct
      901 tgacgtcggg cagtttcagg gacttcatcg cgagcgtgga ggactcgatg cccgtcttga
      961 actcgcgccc gtcgacggtc agcatcaggc gtgccttgct gaccttcttt ccgagcttga
     1021 gagaaagcat tacctcggat gattgagtcg gttcgtcaac cttgatcgcg ctcatttccc
     1081 tggccgggtt ctctccgtca agcgtcacgg atctttcgaa ggcaacgccg atgacctgat
     1141 cgccaacctt gaccgactgg tcggaatagg cgcagaagag cagccatgta aggaactcac
     1201 gtcccaggaa ctgtgtcctg ccgatcttgt cggccatggt gatttcaggc attttctatt
     1261 tccccttcat cgtccgcgca aggttcggga tcaaacgtgg cggttcccgg ctcgatcgtg
     1321 ccgccaaaaa ggaagctggt aggcagcagg tgccagacct gttcaatttc ccgctcatcc
     1381 agccggcttt cgaggattcc gaccgggttc atggcctgaa gcttcagatc caggaactgg
     1441 ttgaagcgca ccaggaaggc ctcgttcagc gactgggaca gggagtagaa tcttatgatt
     1501 cccctggcca tgtcccagat tacgtcaacc gcgttgatcg acgggagggc ccgccgcagg
     1561 agttccagcc gaacctgttc ggaaagttcg tcacgctcct cgcggggcat cctggatctg
     1621 cttgacgccg cgagccgatc ctgcaggcgc ttcctggtgt atatgcgaag cgttccggca
     1681 ggaacggcca gcttgtcaac ccggaacgag aaaaccgcca gcggatcgaa gaagatgttc
     1741 tgctccgtga attcggtctc cagcggatct tcgaaagtgg caaatccaaa cgactgatcg
     1801 tgtccaagat cgacgtcgat cggactgatg cggccgacgg tcagtccgcg caggatggac
     1861 tggcgcagtt cctcgccatt cacatcttcg gtgacccgat aagtcacgta gcttaccgtc
     1921 tggttgttga atcccatgtt cgtttcctta tgatatcagt cctgttcatg taccgagatc
     1981 accctggtca ggcgatccgg ttcgagcctg atgcttatca tgaaatcgac ctgcgcagcc
     2041 acgtccggga ttttgtccgc ccattccacg aggctgacgg cgcccgatga agtgaactcg
     2101 tagtatccgg tcgattcaag atccccggcg tcggtaatcc tgtaaaggtc gaagtggtgc
     2161 accggaatgc gtgcccgata gatgttcatg atcgtgaagg tcgggctgtg aacctgcgtg
     2221 gggtcgcctc cgagcgccct cacggcgcca cggacgaaca cggtttttcc ggcgcccagt
     2281 tcgccgacaa ggccgatgac ttcgcccgga gacgccatcc ggagcagttc cgcagccgcg
     2341 gcctctgttt cctcttcgga agtcgtgcgg tattcgcgca ggattctcat cggcaatccc
     2401 cggtcagacg tactcgacag ccacgatcga aagtcgcctt tcgcccctgg gcgtcttgaa
     2461 cttcacgtcg tcaccgtcct ccttgccgat aagggcccgt ccgagcggtg cgttcaccga
     2521 aatcatgttc cggtcgatgt ccgcttccgc atcgcccacg attctgtaaa cgatttcttc
     2581 gtccctgtct tcgtcgaata tcgtgacggt cgccccgaat cggatgcgct tgtcgcccct
     2641 gaagcgtgac gggtctatta cttcagcccg cgcgataagg ctttccaggt gctttatgtt
     2701 ggcatcaatc agtccctgcc gctcgcgtgc cgagtggtat tcggcgtttt cggacagatc
     2761 acccagatcg cgggcctcgc cgatagcctt gatgattatc ggcatttctt ccttccacct
     2821 cttcaggtct tcctgaagcc tggcgaagcc ttccggcgtc aacggaaccc ttgattcatc
     2881 catatcatgg aacctctgtc ttcacattac gcttccgcct gccgcaaccc ggccggcagg
     2941 aagggtgatt atatacgtca tggcatgtca gtgaaagtcg cggcccgggt gattgacaag
     3001 ctttggcagc ctttcgaacg aatcggcaag caggcttgtg acaccggaac gattctgcac
     3061 gacgccggaa aaaatcagga atgacgaagt aagaagcagt gtccggtttc gcgcgaacac
     3121 ggcgggactg acggcgaccg ccgcatgacc gctttcatcc tctacggtcg cgaaatagaa
     3181 gccctttgcc gttggcggtc gctgcttgac gattatcatg cccgccacgc ttgcgtgcgc
     3241 gccgtcgggc agaagcgaaa gcccgtctgc gggcttaatc ccgcgaacct cgagttgagg
     3301 gcggatgaag gcaatcgggt gcggcccggt ggtcaacccg gttgccctga aatcattggc
     3361 catgtcttca aatgagtgtt caagaagcgg ttcaacacat tccggcatgt cggtgtccga
     3421 ctggtttcgg tgcaaaattg ttgccgatgg gtcaaatgcc attgcctgcc acaatgcgtc
     3481 ccgccttgtc ccccttcctt ccgtcaacag ccccggcccg ctcgccggag ccgccccggc
     3541 atccgcgata atcatcagtt cggatcgccc ggccagcgcg gcctcgccga catgtgtaat
     3601 gtcacgataa ccacccgtcg gccgcttccg gatgatgcga tcgattgtcg atgcggccag
     3661 tcccctgatg aatttcaggc cgagccttat tgcgattttt ccatccgcgg catgttcaag
     3721 ggtgcagtcc gaagcagatt tgttgacgtc gatgggcaga accggaacgt tgtggtgcat
     3781 ggcgtccctt accagcgtcg ccggcgaata gaatcccatc ggccagcagt tcaggaggga
     3841 tgccgtgaat gccgcgtgat ggtgaaattt cagccacgcg cttgcccatg cgataagggc
     3901 aaacgacgcg gcatggcttt ccgggaagcc gtacagcgcg aacgaaatga ttgaagagac
     3961 gatttcgtcg cgggcaggct tttcgatacc gttgcgttcc atccccgcgt gcagcttttc
     4021 caccagccgc agcatccttg cccgggaacg cctggaaccc atggcgcggc gcaattcttc
     4081 ggattcgccg ccggaaaatc cggccgccgt catggccatg cgcatcaatt gctcctggaa
     4141 gagcggaatt ccgagcgtgc gctcaagtat cggctgcagg cagggcgcgg cgtatctgac
     4201 cggggccgcc ccgcatcggc gctcgagaaa gggatggacc atctttccga caatcggccc
     4261 tggcctgatg atggcgactt cgatgacaag atcgtagaag cagcgcggcc tcattcgagg
     4321 gagcgttgcc atctgcgcgc gcgactcgat ctggaatacg ccgatcgtgt ctgcgcggca
     4381 gagcatgtcc cagacatcct tgtcgtttac ggggattcca gcgagatcca cgtcgactcc
     4441 ctggtggcgg gaaacaagct cgatggtctt ggaaatggcg gcaagcatgc ccagaccaag
     4501 cagatcgacc ttgatcatcc gcatgtcggc gatgtcgtcc ttgtcccact ggattacacg
     4561 cctgccggcc atcctggccg ggacgatcgg cacaaccctg tcaagcctgt ttcccgagat
     4621 gacaaggcca ccgttgtgtt gtccgatgtg tcggggaaga tcctgcatct ggtgccagac
     4681 gtcgatgaaa gactggacgg ttcggtcgcc tggatccagt cccgcctggg aaaccttgtt
     4741 cagaaaggac tcctggtcat tagaaaa
//
LOCUS       DAOPPA010000239         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19516_length_4767_cov_3.820883, whole genome shotgun sequence.
ACCESSION   DAOPPA010000239 DAOPPA010000000
VERSION     DAOPPA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19516_length_4767_cov_3.820883"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKH54_08150"
     CDS             complement(<1..841)
                     /locus_tag="PKH54_08150"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOC99903.1"
                     /translation="METPGRSRFRLWVATCAIIDLVWPCAGRALLHRPPAGRWGMIWW
                     MGFLVILSMWTISMMINALQALLVAAAIWATVQIHLQSRNIESIIRGPCPGELADFER
                     RFSGRKLSILLILLVYVVTPTVFIGRAEKRLSILTVHDFGTFPSILPGESLLVLKSGT
                     RPAKAGELVVFNSEHGTSIGRVLATAGKTVSVSEDGVVIDGVPVPSTSPESISFDDFV
                     LTEAESTEARGLMVYSEFSGEGINPAQVFIRKDAVINRPDVVVPEGTVYVLADNRSTT
                     HSTD"
     gene            complement(842..1501)
                     /locus_tag="PKH54_08155"
     CDS             complement(842..1501)
                     /locus_tag="PKH54_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOC99904.1"
                     /translation="MTCNMMGIYGLLLEDGGHQHWWPGETAFEVAVGAVLTQNTSFSG
                     VTKAIDRMKAAGLMSPAAIVAAPDDIVGQAIRPSGYFNVKTARLKALCRFLLENDAAD
                     PASLARFPVPRLRQMLLAVHGVGRETADSIILYATNRPIFVIDAYTRRIFSRMGLVQA
                     DADYDDLRDFFERSLPRDAAIYNDFHAQIVRCAKECCRKRPNCGRCALRQSCRTGMAE
                     A"
     gene            complement(1498..2727)
                     /gene="tatC"
                     /locus_tag="PKH54_08160"
     CDS             complement(1498..2727)
                     /gene="tatC"
                     /locus_tag="PKH54_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOC99905.1"
                     /translation="MKAEATEPADAEMPLAGHLAELQGVLIQIFIALLVAFAASWAFH
                     QQVFYFLAAPVLDGLARHGIDRLQALDVTEGIVIHMKASGVAALLVTFPFIIWRLWRF
                     VAPALHIHEKRAVIISGMLTALFFILGTTFCYTVFLPLVVEFLAGFGTSDGTLVLAPT
                     ISNTFGTAMVFLVAFGIIFELPLVMMALGALHLIRPRWMLARSRYFIVAAFIIGAIFT
                     PPEPVSQTLMAVPICLLYFLGVLLAWAGWKLGDGASRRSAVVVTSAIVLFTAGIVGAA
                     VLLAGRHDAGDALTGGAERDVAGTAVTTVEIDSDCFFTISGLQVKGPVLVTSTAGDRQ
                     MAGVAFDLPSEKATFSFKGARGAVGEFVNQERQWTASGGRVLAMMLTDSRRVAEFMSA
                     LVSETTTNCRGTEGQVQ"
     gene            complement(2708..2932)
                     /locus_tag="PKH54_08165"
     CDS             complement(2708..2932)
                     /locus_tag="PKH54_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOC99906.1"
                     /translation="MMGIGWTEILLILGVALVVLGPTRLPSLARTLGRAVREFRSSFA
                     ALEDDLEGRNAPTDPAKKADGRGSDESGSD"
     gene            3096..4247
                     /locus_tag="PKH54_08170"
     CDS             3096..4247
                     /locus_tag="PKH54_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC99907.1"
                     /translation="MKDGWIVCGSGPEGFASWEFGLSGGRFIGDGRYAAPGTDGVSVG
                     GILIGPGRAARVGAAVYSCLPARFGTGGIREARLGRMAGASDAMLQVMHSAAAAALSN
                     ASVLILGESGTGKELVAEYLGSIAGSMHGINMGAVPGDLAEAELFGWMRGSFTGAVDS
                     RAGALEAAGCGVLFLDEIGEAPAWVQVKLLRALDTRKFRRLGGGRDVELKARVVAATN
                     LDPIEGIASGRLRLDLLERLACHVIRMPPLRSRPADIPCLLRLFSAAAVQGVRDQVPQ
                     PSPQVIDLLRRWPWHGNVREFRNVVERVMLASLDGMSSERLVNAALEQGRVFHAPGCD
                     EASVPASSRADEISASGLPRSTYYYRLKRGRIPSDQGPRRSVTASASRT"
     gene            complement(4222..>4767)
                     /locus_tag="PKH54_08175"
     CDS             complement(4222..>4767)
                     /locus_tag="PKH54_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOC99908.1"
                     /translation="PVNAALKQEEPVPADVGGSGAVAPEPPAPGQGAESMTSHGVGLA
                     GIASSDDPDEGRCVGACGGRGVQGGGSADTARNPSPGSAGGDDGTGRYALMVRKLLER
                     RGAYPGTARRLGLEGLVRMTVTVDADGSILDTKIERSSGFRELDQAALASARDVSGLP
                     PPPGGRPLRILIPVRFSMRRL"
BASE COUNT          936 a         1487 c         1473 g          871 t
ORIGIN      
        1 aatccgtcga atgcgtggtg cttcggttgt cggccagaac atagacggtg ccttcgggaa
       61 cgaccacgtc cggccggttg atcacggcgt ccttccggat gaaaacctgg gccgggttga
      121 tgccttcgcc actgaattct gaataaacca tcaggccgcg ggcctccgta gattcggctt
      181 cggtaagcac gaaatcgtca aacgaaattg attccgggga agtggacggc accggaacgc
      241 cgtcgatgac aacgccgtcc tcggataccg aaacggtctt tccggccgtc gccagcacgc
      301 gcccgatcga ggttccgtgc tcggaattga ataccacgag ctccccggcc ttcgcgggcc
      361 gtgttcccga cttcagcacc agcaggcttt caccgggaag aatcgatgga aaggtgccga
      421 aatcgtgcac cgtaaggatg gaaagacgct tttcggccct gccgatgaat accgtcgggg
      481 ttacgacata taccagcagg atcaacagta tggagagttt gcgaccggag aacctgcgtt
      541 cgaaatcggc cagttcgccg gggcacggcc cgcgaataat cgattcgatg ttcctggact
      601 gaaggtggat ttgcacggtg gcccagattg ccgcagccac aagaagtgcc tgcagggcgt
      661 tgatcatcat cgatatggtc cacatcgaca ggatgaccag gaatcccatc caccagatca
      721 ttccccatcg tcccgcgggc ggccggtgaa gcaatgccct tccggcacat ggccagacca
      781 ggtcgatgat cgcgcacgtc gcgacccaga gccggaatct ggatctgcca ggtgtttcca
      841 atcaagcctc cgccattccg gtccggcacg attgccgcag tgcacatctg ccgcaattcg
      901 ggcgcttccg acagcattcc ttcgcgcatc gcacaatctg tgcgtgaaag tcattgtaaa
      961 tcgccgcgtc acgcggcagg gaccgctcga agaagtcccg caggtcatcg tagtcggcat
     1021 ccgcctggac aagtcccatc cttgaaaaaa tccgtcttgt ataggcgtcg atgacgaaaa
     1081 tcgggcggtt cgtggcatac agtatgatcg agtcggcagt ctcgcgtccg acgccatgaa
     1141 cggcgagcag catctggcgc agccggggaa ccgggaatct ggcaagactg gcgggatccg
     1201 ccgcgtcgtt ttcaagaagg aagcggcaca gggccttcag cctggccgtc ttcacgttga
     1261 aatagccgga tggcctgatc gcctggccga cgatgtcgtc aggggccgcg actattgccg
     1321 cggggctcat cagcccggcc gccttcatcc ggtcgattgc ctttgtgacg ccggaaaatg
     1381 acgtgttctg cgtaagaacc gcccccacgg cgacctcgaa ggcggtttca ccgggccacc
     1441 agtgctggtg cccgccgtct tcaagaagca atccgtatat gcccatcatg ttgcaagtca
     1501 ttgaacctgc ccttccgtgc ctcggcagtt cgtcgtcgtc tcgctcacca gcgcagacat
     1561 gaattctgcc acccgccgcg agtcagtcaa catcattgcc aggactcggc caccagatgc
     1621 cgtccactgc ctttcctggt tgacgaattc cccgacagcc ccgcgggcgc ccttgaatga
     1681 aaatgtcgcc ttttcggaag gcaggtcgaa cgccacgccg gccatctgcc gatcgccggc
     1741 ggtcgaggtc acaagaaccg ggccttttac ttgcagcccg ctgatcgtga agaagcagtc
     1801 ggaatcgatt tcaaccgtcg tgaccgccgt ccccgcgaca tccctttccg ccccgccggt
     1861 caaggcgtcg ccagcgtcat gccttccggc aagaagtaca gccgcaccga cgattccggc
     1921 cgtgaaaagc actatcgcag atgtaacgac gacggcggaa cgccgggacg ccccgtcgcc
     1981 aagtttccat ccggcccagg caagcagaac cccgagaaag tacagcaggc agattggcac
     2041 ggccatcagc gtctgggaaa ccggttccgg cggcgtgaag atggcgccga tgatgaaggc
     2101 ggcgacgatg aagtaccttg acctggcaag catccagcgc ggtcggatca ggtgcagcgc
     2161 ccccagggcc atcatcacga gaggcagttc gaagatgatt ccgaaagcca ccaggaatac
     2221 catcgccgtc ccgaatgtgt tcgagatggt tggcgccagc accagggttc cgtcggacgt
     2281 tccgaatcct gcaaggaatt cgaccacaag cggaagaaaa acggtgtaac agaaagtcgt
     2341 tccgaggatg aaaaagagcg cggtcagcat tcccgaaatg atgacggccc gcttttcgtg
     2401 aatgtgaagc gccggggcga cgaagcgcca taaccgccag atgatgaaag ggaaagtgac
     2461 cagcagcgcc gcgaccccgg acgccttcat atggatgacg atgccttcgg tgacgtccag
     2521 tgcctgcagg cggtcgatgc cgtgtcttgc aagtccgtca agaaccgggg ctgccaggaa
     2581 gtaaaagacc tgttgatgga atgcccagga tgcggcgaat gccacaagca gggcgatgaa
     2641 gatctggatc agaacccctt gaagttccgc caggtggcct gcaagcggca tctccgcgtc
     2701 ggccggttca gtcgcttccg ctttcatccg aacccctgcc gtcagccttc ttcgcgggat
     2761 ccgtcggcgc gttccgtcct tccaggtcgt cctcgagcgc ggcaaacgac gacctgaatt
     2821 cccgtacggc cctgccgagc gtccttgcca gcgatgggag cctggtcggg ccgagcacca
     2881 ccagggcgac cccgaggatc agcaagattt ccgtccatcc gattcccatc atgccccccg
     2941 gcgtaccaat cacgggaatt cataactagc agaccgtcca agccatgtca aagggcgtgc
     3001 cggccgccgc gcatcacatt ccgccgacag ggtgaaacag aatccagtcg ccaatcgaat
     3061 ctgatgccac ggacagcttc aggacggggt taatcatgaa ggacggatgg attgtctgcg
     3121 gcagtggccc ggaaggcttc gcgtcatggg aattcggcct gtcgggcggc cgcttcatcg
     3181 gcgacggcag gtatgcggct cccggaaccg acggcgtctc cgtgggcggg atcctgatcg
     3241 gcccgggacg ggcggcccgc gtcggggcgg ctgtctattc ctgccttccg gcaaggttcg
     3301 ggactggtgg catccgggaa gcaaggcttg gacggatggc cggggcatcg gacgcgatgc
     3361 tccaggtgat gcattcggcg gcggcggccg cgctttcaaa cgcttcggtt ctgatcctgg
     3421 gcgagtcggg aaccggcaag gaacttgttg ccgagtacct ggggagcatc gccggatcca
     3481 tgcatgggat caacatgggt gcggttccgg gcgacctggc ggaagcagag cttttcggat
     3541 ggatgcgcgg atccttcacg ggtgcggtcg attcccgggc cggcgcgctt gaagccgccg
     3601 gctgcggcgt gctgttcctt gatgaaatcg gcgaggcgcc ggcatgggtt caggtcaaac
     3661 tgctgcgcgc cctggataca aggaagttca ggcgcctcgg tggcggtcgg gacgtggaac
     3721 tgaaggccag ggtcgtcgcg gccaccaacc ttgatccgat cgagggaatt gcttccggcc
     3781 ggttgcgcct ggatctgttg gagcgtcttg cctgtcacgt gattcgaatg ccgcccctgc
     3841 ggtcgcgccc ggccgacatc ccgtgccttc tgcgactttt ctccgcggcc gccgtccagg
     3901 gcgtgcgcga tcaggttccc cagccatccc cccaggtgat cgacctgctt cgtcgctggc
     3961 catggcacgg aaacgtgcgt gaattcagga acgtggttga acgggtgatg cttgccagcc
     4021 tggatggaat gtccagcgaa cgcctggtca acgcggcctt ggagcagggg cgggtgtttc
     4081 acgctcccgg ctgtgatgaa gcgtccgtgc cggccagttc aagagccgat gaaatcagcg
     4141 cgagcggtct tccaagatcg acctattact accgtctgaa aaggggaagg atcccgtccg
     4201 accagggtcc gaggcggtcg gtcacagcct ccgcatcgag aacctgaccg ggatcaggat
     4261 cctcaacggg cgtccgcctg gtggcggcgg cagtccggaa acgtccctgg cggatgcgag
     4321 ggcagcctga tccagctcgc ggaacccgga tgaacgttca attttcgtgt ccaggatcga
     4381 cccgtccgcg tcaacggtga cagtcattct taccaggccc tccagtccaa gcctcctggc
     4441 ggttccgggg taagcacccc tgcgctcaag cagctttctg accatcaggg cgtaccgtcc
     4501 ggttccatcg tcaccacctg ctgagccggg agatggattc cgggccgtgt ccgcagagcc
     4561 gcccccttga acgccgcgac caccgcacgc gccgacgcag cgaccttcgt ccgggtcatc
     4621 gcttgaggcg atgccggcca aaccgacacc gtgagacgtc atggattcgg caccctggcc
     4681 cggcgcgggt ggttccggtg caacggcccc cgaccccccg acatcagcgg gcaccggttc
     4741 ttcctgcttc aatgcggcat ttacggg
//
LOCUS       DAOPPA010000240         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19517_length_4767_cov_3.171265, whole genome shotgun sequence.
ACCESSION   DAOPPA010000240 DAOPPA010000000
VERSION     DAOPPA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19517_length_4767_cov_3.171265"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKH54_08180"
     CDS             complement(<1..301)
                     /locus_tag="PKH54_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOC99909.1"
                     /translation="MCTRDENCENDDITHPDESLLIEGHPVLDFERGPIVRFTFNGRP
                     IEGFEGQPVLVSLRAAGVLKVRESPKKHLPSGPFCMQGRCCSCMMTVDGSPNVMTC"
     gene            complement(563..2689)
                     /locus_tag="PKH54_08185"
     CDS             complement(563..2689)
                     /locus_tag="PKH54_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99910.1"
                     /translation="MKRTLTILFVAVALTQCGTGENTVTDAGLDAVETTDLNGDAATD
                     AIANPDADPADAVADVPDGAGDLGDDDTDGDVDAKQDAGDTDDAGDVKELPPYDEPPV
                     RVLFDLLGAQVESPFPSNHYIDPETGTIRIASNGYNNSLLYLIEMDFPTYAQDAARAE
                     GFALYSALAFLTSTAVDPESIPGTGQASLQPDSSVKLFRIDGETPVKVAIHCAFKRFE
                     ESRMFNLTQCFPLYRLEPDATYLFVVSNDLKDIDGNAFARSRGFMQALGLARVEVPAT
                     AERLELMRHTGQQVELGMSGLPADAPADDMVAATVFSTGHPNVDMEDVMKNFRQDAPM
                     LTVEYELDEDGEGNPRIFTGSTLPGCSMPDADMEWGLHGYFTAPEFRNTDGHFERDGD
                     GSFRTFPGERIRFTLMVPEGDGPFPVVIAQHGVSSTESAMCNFGRELVRRDVAVMRFL
                     WPGHGGDNERGNGTTDFLDVLNPARIAYNFMQSATEIASAVILLDKLNQDMAGLRPEG
                     DGLPPLDTGRIGYVGTSLGSIIGLLYLPFSDRINILVSNVGGLGMSHLVKQFVDEYFG
                     GIYVGSAVTNLADHIVAPGDGISNAEKILLEPVEWASGPKYVLAQEVIGDAVIANACT
                     EVLARNAGLKLVNPVAVPVEGLESDDPENLTSGLFQFTGTDHHQFPNDESDPVAKTER
                     SQAWHYLETGLKNGVPEIKVFSPDTL"
     gene            3014..3892
                     /locus_tag="PKH54_08190"
     CDS             3014..3892
                     /locus_tag="PKH54_08190"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOC99911.1"
                     /translation="MQYRQLTPEEERVIIHKGTERPFTGRYWNHHEDGTYSCRRCNAP
                     LFLSSSKFDSGCGWPSFDSAIKGAVREIPDADGLRTEIVCAACGAHLGHVFRGEGFTE
                     RDTRHCVNSVSIDFAGSSKQPAAEAVMSAGQTRDAYFAGGCFWGVEYWFDREPGVIRA
                     ESGYMGGALSNPSYEQVRTGATGHAETVHIVFDPAVTSYEKLARLFMEIHDPTQVNRQ
                     GPDVGHQYRSAIYVTDESQKQVVEGLIEQLKARGLKVQTEVEPAGTFWPAEEYHQDYY
                     QRKGSEPYCHSRVKRF"
     gene            4060..>4767
                     /locus_tag="PKH54_08195"
     CDS             4060..>4767
                     /locus_tag="PKH54_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOC99912.1"
                     /translation="MEKLEGSQAAGGFASNEVVFYVPVPEDVVGLDAFDAGEQEILDV
                     LNRKVASGDSLDSVMDFLYETSHGVWPCDRIGLAFVEDDGRRVTSRWVRAEYEPVIMG
                     SGYTEDLEGSTLKPLIDGKVLRVITDMEEYGRRNPASRSTKVLLREGVMSSMTCPLVV
                     DGRCVGLLFRSSRKKGVYGPREVWMHLAVSERIAQAVEKAWRIRQLEQINQAYMEMLG
                     FVAHELKSPVASMVLDAT"
BASE COUNT          903 a         1391 c         1476 g          997 t
ORIGIN      
        1 cgcaggtcat cacgttcggg ctgccgtcaa cggtcatcat gcaactgcag cagcgtccct
       61 gcatgcagaa ggggccggac ggcaggtgct tcttcgggct ttcccgaact ttcaggacac
      121 cggccgcgcg aagggaaacc agcaccggct gtccttcgaa gccctcgatc ggccggccgt
      181 tgaaagtgaa tcggacaatg ggtccgcgtt caaagtccag aaccggatgt ccttcaatca
      241 gcagcgattc gtctgggtgg gtgatatcgt cgttttcgca gttttcatcg cgggtgcaca
      301 tgattttcga atacctcgat ttccagtggt gcaacaaccg ggtgacacga aaccacaacg
      361 gcccgaagac gtcaagccgc gcgaacgtca agctgttgga tttttcactg catccggcca
      421 tgaagtgtcc ggttcgaggg gccaaaccgg agcacttatg gtccgtcccg atccgccccc
      481 cgatccgtac gggcgaatga aaattcgccc gcctgcgtga aaattcgttt gccgtgcggc
      541 ggcgagtgat tatggcgttt tcttacaacg tgtccgggga aaataccttg atttcgggga
      601 cgccgttctt caggccggtt tccaggtaat gccacgcctg gcttcgctcg gtcttcgcga
      661 ccgggtcgct ttcgtcgttc gggaactggt ggtggtcggt tccggtgaac tggaacaggc
      721 cggacgtcag gttttcgggg tcgtccgatt cgagcccctc gaccggcacc gcgaccgggt
      781 tcaccagctt caggccggca ttgcgggcaa ggacctcggt gcacgcgttc gcgatgaccg
      841 cgtctccgat gacttcctgg gccagcacat atttcgggcc ggatgcccat tcgaccggtt
      901 ccagcaggat cttttcggca ttcgagatgc cgtcgccggg ggccacgatg tgatccgcaa
      961 ggttcgtcac cgcgctgccg acataaatcc cgccgaagta ttcatcgacg aactgcttga
     1021 ccaggtgcga catcccaagt ccgccgacgt tcgataccag gatgttgatt cggtcggaaa
     1081 acggcaggta cagcaggccg atgatcgacc cgagcgacgt gccgacatac ccgatccgtc
     1141 cggtgtccag gggcggcagg ccgtcgcctt ccggacgcag gccggccatg tcctggttca
     1201 gcttgtccag caggatgacg gcggacgcga tttcggtcgc cgactgcatg aagttgtaag
     1261 cgattcttgc cgggttcagc acgtccagga agtcggtcgt cccgttgccg cgttcgttgt
     1321 cgccgccgtg tcccggccac aggaagcgca tcactgccac gtcgcgcctt acaagctcgc
     1381 gcccgaagtt gcacatcgcg ctttcggtcg atgacacgcc gtgttgcgcg atcaccaccg
     1441 ggaacggtcc gtcgccttcg ggaaccataa gggtgaaccg gattctttcg ccggggaacg
     1501 tccggaaact tccgtccccg tcacgttcga agtgtccgtc cgtgttacgg aattctggtg
     1561 ccgtaaagta tccgtgcagt ccccattcca tgtccgcgtc cggcatgctg cagccgggaa
     1621 gcgtggagcc ggtgaagatc ctcgggttgc cttcgccatc ctcgtccagt tcgtactcga
     1681 ccgtcagcat cggggcatcc tgcctgaagt tcttcatcac gtcttccata tcgacgtttg
     1741 gatgtccggt cgaaaagacc gtcgcggcca ccatgtcatc ggccggggcg tccgccggaa
     1801 gcccggacat ccccagttca acctgctgcc cggtgtggcg catcagttca aggcgttcgg
     1861 cggtggccgg cacctcgacg cgtgccagcc caagggcctg catgaacccg cgtgaccggg
     1921 cgaatgcatt gccgtcgatg tccttcaggt cgttgctgac cacgaacagg taggtggcgt
     1981 ccggttccag cctgtaaagc gggaagcatt gcgtcaggtt gaacatcctt gattcctcga
     2041 accgcttgaa ggcacagtga atcgccacct ttaccggcgt ttccccgtca atcctgaaaa
     2101 gttttaccga tgaatccggc tgcagggatg cctggccggt cccgggaatc gattcgggat
     2161 cgacggccgt cgaagtcagg aaggcaagcg ccgagtagag tgcgaaccct tccgcccggg
     2221 ccgcgtcctg cgcataggtc ggaaagtcca tctcgatcag gtaaagcagc gagttgttgt
     2281 atccgttcga agcgatcctg atggttcccg tttcgggatc gatgtagtgg ttcgacggga
     2341 agggggattc cacctgggcg ccaagcaggt cgaaaaggac cctgaccggc ggctcgtcgt
     2401 acggcggaag ttccttaacg tcgccggcat cgtccgtatc ccccgcatcc tgcttggcgt
     2461 caacgtcgcc atccgtgtcg tcgtcgccca ggtcgcccgc cccgtccgga acatcggcca
     2521 ccgcatccgc gggatcggcg tccggattcg cgatggcatc cgtggccgca tcgccgttta
     2581 agtcggtcgt ttcaacggcg tccaggccgg catcggtaac ggtgttttcg cccgtgccgc
     2641 attgcgtcag cgcaaccgcc acgaacagga tggtcaacgt tcttttcata atggttccgg
     2701 catttcagat aagtcttgga taaagaaggc accagccttc gccgcgcgta ttatgccgcg
     2761 tggccgccac aaagtgaacg tcgaatgttt cgtgcgtctt cgcgtcataa gtcctgaaaa
     2821 accggacaaa aattgtcgca taagcattgc catttccgga ttaatgcaga ctttccttgt
     2881 cagggccttt cgaaatgggg gcatcgtgat gaaatcatgg attttccggc ctttgccggc
     2941 ggtcggggcg ctgatgctgg ccaccgtggt gttcgcatcg tgtccggcca gtcctgaaag
     3001 cggggataag acaatgcaat acaggcagct tacaccggaa gaagaaaggg tgataatcca
     3061 caagggcacc gaacgtccgt tcacgggcag gtactggaac caccacgaag acggcacgta
     3121 ttcgtgcagg cggtgcaacg cgccgctttt cctgtcttcg tcgaaattcg attccggatg
     3181 cggatggccc agcttcgatt cggcgattaa gggcgcggtg cgtgaaatcc cggacgcgga
     3241 cggtttaaga accgaaatcg tatgcgcggc gtgtggcgcc caccttgggc acgtgtttcg
     3301 cggcgagggt ttcaccgaaa gggacacgcg tcattgcgtg aattcggttt caatcgattt
     3361 cgccggcagt tcgaaacagc cggcggcgga ggctgtcatg tcggcggggc agacaaggga
     3421 cgcgtatttc gcgggtgggt gcttctgggg cgtcgaatac tggttcgatc gtgaaccggg
     3481 cgtcatcagg gcggaatcgg gatacatggg cggcgcgctt tcgaaccctt cgtacgagca
     3541 ggtaaggacc ggcgcgaccg gccacgcgga aacggtgcac atcgtcttcg atccggccgt
     3601 gacttcgtat gaaaagctgg cccgcctgtt catggaaatc cacgacccga cacaggtgaa
     3661 ccgccagggg ccggacgtcg gccaccagta caggtcggcc atttacgtga ccgatgaatc
     3721 gcagaagcag gttgtcgaag gcctgattga acagttgaag gccagggggc tgaaggtcca
     3781 gaccgaggtc gagccagccg gaaccttctg gccggcagag gaatatcatc aggactacta
     3841 ccagcgaaag ggatccgaac cttactgcca ctcccgcgtg aaacggttct gacccaagcg
     3901 ccagttggtc accgcggccg actcgcgcca ctcccgcgtg aaacggttct gaccgcttaa
     3961 tttgtcgcgg ttttttccaa agacgattgc ggcccggccg gttgcaacct atcatcatcc
     4021 ggatggcatt ttgcacttgg tttctggcgg ggtttcttca tggaaaagct ggaaggttcg
     4081 caggccgctg ggggctttgc ttcaaacgaa gtggtctttt acgtcccggt tcccgaggat
     4141 gttgtcggtc ttgacgcctt cgacgccggc gagcaggaga tcctggacgt cctgaaccgc
     4201 aaggtcgcaa gcggcgattc gctggattcg gtcatggatt tcctttacga gacttcccac
     4261 ggggtctggc cctgcgaccg catcgggctt gcctttgtcg aggacgacgg ccgtcgcgtt
     4321 acttcgcggt gggtgcgcgc cgaatacgag ccggtgatca tgggatccgg ctataccgag
     4381 gatctggaag gcagcacgtt aaagcctttg attgacggca aggtgctgcg cgtcatcacc
     4441 gacatggagg aatacggccg ccgcaatccc gcgtcgcgat cgacgaaggt gctgctgcgc
     4501 gagggcgtca tgtcgagcat gacgtgcccg cttgtcgtcg atggccggtg tgtgggtctt
     4561 ctgttccgaa gttcccggaa aaagggcgtt tatggcccaa gggaagtctg gatgcatctg
     4621 gccgtttccg aaaggattgc gcaggcggtc gaaaaggcct ggcgcatccg gcaacttgaa
     4681 cagatcaacc aggcgtacat ggagatgctg ggcttcgtgg cccacgaact gaaaagcccg
     4741 gtcgcatcga tggtgcttga tgcaacg
//
LOCUS       DAOPPA010000241         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19607_length_4748_cov_4.165140, whole genome shotgun sequence.
ACCESSION   DAOPPA010000241 DAOPPA010000000
VERSION     DAOPPA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19607_length_4748_cov_4.165140"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKH54_08200"
     CDS             <1..250
                     /locus_tag="PKH54_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99913.1"
                     /translation="PIVLVVITNILGSVFYQTAFANGRASIVAAICTIVGNTVPIVAA
                     ITIFGEKVRPMHGAGIVIVLLGAFVLAVGNRRAAVRGG"
     gene            252..1928
                     /locus_tag="PKH54_08205"
     CDS             252..1928
                     /locus_tag="PKH54_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOC99914.1"
                     /translation="MARTALDLSPEIIVTVNGTGYSTSRGTRVKNFLRRYLPAVEPDC
                     LGAIVDNRLVHIEYPLADDCELIPVTYDSPHGVRIYRSTLSIMLGEAVARLFPSASVR
                     IGQSFGRSFYYDVIREGGVSPADIAAIESEMKAMVARNEPLQVTRLPRGKAMRIFEAA
                     GRMHSAGLLKVVVRKWVPLVSMGVRVDLCMNPVTPTAGYIRQFRLDPLGPGLVLNIPR
                     RGNPSRPPEPVDCPPSLFRSFRETRDWNALVGVESVADLNATVLDGSIGEVIRVSEAL
                     HERKLAAIADEIASRKARLVAVAGPSSSGKTTTIRRLGMQMKALGLRPKALSLDDYYR
                     NRSDTPRDENGELDFEHIEAIDLDLLNEHLQGLLEGATVQTPRFSFQQGMRSPSRVPM
                     SLASDEILLVEGIHGLNDRLTSAVPDDRKFRVFCSAVTQLCIDDQHRVFTTDVRLIRR
                     IVRDRHFRGYSAADTLRLWPKVRAGEKKWIFPWQDRADVFFNSTLVYEPAVLKVFAER
                     FLIEVSDTDPVWVEAERLLKFLRFFVPVFTDDVPGTSIIREFVGESSFRY"
     gene            2160..3554
                     /locus_tag="PKH54_08210"
     CDS             2160..3554
                     /locus_tag="PKH54_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOC99915.1"
                     /translation="MSFAGIRNCGVPAVLAVMIFTLLAPMAAMASGGSAPYHVPVYAV
                     VPFVLLLLCIAIMPLKFPHFWESNAKKGIVVAILSVPIVGFYLYQNDVHRLLEHLHEY
                     LSFIVLLWSLYTISGGIVLRGNLKASPAVNTAFLGIGALIANLFGTTGASMLLIRPLL
                     RTNQEREFKVHTVVFFIFVVSNIGGCLTPLGDPPLYMGFMRGVPFQWTLSLWPQWLLV
                     NSLLLVIYYMWDRIRYRQEKAENVAMDMTNAEPLRIDGKINIALILGVVATIALSGVL
                     EMNPWLRDGILVALGLLSMATTKKKYREDNEFNFGAITEVAVLFIGIFITMIPALMML
                     SEAGPGLGVNHGWQYFWITGTLSSFLDNTPTYIVFLDLAQSTLGSSTIEAFMSHVEGP
                     AILKAISLGAVFMGANTYIGNGPNFMVKAIAEAKGSSQVKMPSFGGYMVYSMLILVPI
                     FLLVTLLTFVLGIM"
     gene            complement(3547..>4748)
                     /locus_tag="PKH54_08215"
     CDS             complement(3547..>4748)
                     /locus_tag="PKH54_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOC99916.1"
                     /translation="GALVFIWTFYGIEGLFRKIRIPFLLKPAIGGLLTGGVALALIEL
                     SPDDRVMDVLSTGYGVLQDALSGEPSRMAALILLVVATGKILTTSLTIGSGGSAGVFG
                     PSMVIGGTLGGSAGMVLHSWWPTLVPNPSTFAVVGMAGFFAAAANTPLSAVILVSEMT
                     GGYDLIVPAVWVCSISFLVSRRWTIFRSQVPSRINSPAHIGEYAFEIMGSSKVGDVFK
                     KNRRFTCIARDATPAQVMQRTADTRQRVYPVTSQDGRLLGVFQQSELIQAVHEAGERA
                     RGLVAADLMHPVADSPGWASVRLSDSLMTAHRRMTSRGSEELVVISDDGTDSVAGILT
                     ASDVLIAYNRRLADLSAGSGNADVIPPPEDDEQERADEAERISTEAMAGSELDARGNA
                     ADGSGTT"
BASE COUNT          900 a         1395 c         1417 g         1036 t
ORIGIN      
        1 cccgattgtc ctggtcgtca tcaccaatat ccttgggtcc gttttttacc agaccgcgtt
       61 cgcgaacgga cgcgcaagca ttgtcgccgc gatttgcacg atcgtgggca acacggtgcc
      121 aatcgtggcc gcgattacca tttttggtga aaaggtacgg cccatgcacg gcgcgggaat
      181 cgtgatcgtg ctgcttggtg cattcgtgct cgccgtcggg aatcgcaggg cggcggtccg
      241 gggaggctga aatggcacgg acggcgcttg atctgtcgcc cgagataatc gtcacggtta
      301 atggcacagg ctattcgaca agccggggta ccagggtcaa gaacttcctt cggcgttatc
      361 tgcctgcggt cgagcctgac tgccttggcg cgattgtcga taaccggctg gttcacatcg
      421 agtatccgct ggccgacgat tgcgagctga tccccgtaac ctatgacagc ccccacggtg
      481 tccggattta ccgttcgacc ctgtcgataa tgctgggcga ggcggtcgcc aggctgttcc
      541 catcggcttc ggttcgaatc gggcagtcct ttggcaggtc gttctattac gacgtaatcc
      601 gggaaggcgg cgtttcgccg gccgacatcg cggcaatcga gtccgaaatg aaggccatgg
      661 ttgcccgcaa cgaaccactg caggtcacga gactgccaag gggcaaggcc atgaggatat
      721 tcgaggccgc cggccggatg cattccgccg ggcttctgaa ggtcgtcgtg cgcaaatggg
      781 ttccccttgt ttcgatgggt gtccgggtcg atctgtgcat gaatcccgtc acgccgaccg
      841 ccggatacat caggcagttc aggctggatc ccctggggcc cggcctggtt ctgaacattc
      901 cgcgtcgcgg caacccgtcg aggccgccgg agccggtcga ttgtccgcca agccttttcc
      961 gttcattccg ggaaacaagg gactggaatg cgcttgtggg ggtcgagtcc gtcgctgact
     1021 tgaatgcgac cgtgctcgac ggaagcatcg gcgaggtcat aagggtttcc gaggcccttc
     1081 atgagcgaaa gcttgccgcg atcgccgacg agatcgccag tagaaaggcg cggctggtcg
     1141 ctgtcgcagg tccgtcatcg tctggcaaga caacgacaat ccgtcgcctt ggaatgcaga
     1201 tgaaggccct gggactccgg ccgaaggccc tttcgcttga cgactactac aggaaccgtt
     1261 cggacacgcc ccgcgatgaa aacggtgaac tcgacttcga acatatcgag gcgatcgacc
     1321 tggatctgtt gaacgagcac cttcagggcc tgcttgaagg tgcgacggtt cagacgccaa
     1381 gattttcgtt ccagcaggga atgcggagcc cctccagggt tccgatgagc ctggcttccg
     1441 acgagatcct gcttgtcgaa ggcatccatg gtctgaatga ccggctcaca agcgcggttc
     1501 ccgacgatcg caaattccgg gtcttctgct cggcggtcac gcaactctgc atcgacgatc
     1561 agcaccgcgt cttcacgacc gatgtccggt tgattcggcg aatcgtccgc gatcgtcatt
     1621 tccgcggata ttcggccgcc gataccctgc gcctgtggcc caaagtccgc gccggggaaa
     1681 agaaatggat attcccctgg caggatcgcg ccgacgtgtt cttcaattcc acgctggttt
     1741 atgaaccggc ggtcttgaag gtctttgccg aacgcttcct gatagaggtc agcgacacgg
     1801 atccggtgtg ggtcgaagcc gaacgccttc tgaagttcct gcggttcttc gtgccggtct
     1861 tcaccgacga tgtgccgggc acgtcgatca tccgcgagtt tgtcggcgaa tcatccttca
     1921 ggtactgacc acgtcaccgt cgaagccgtc gatgaacatg cggcaaagca tcctttgtcg
     1981 ggtaccgggc agagcctgtg aaggctgttg ccgcggcagt ccttcggttc tgtgaaattt
     2041 atttcagata cagcgtctcc ggggtgttcc aataaagacg attatagata caatcgcgcc
     2101 cgtgaagcgg tgcggcggca gatgtccgcg cctgggaatg gatttcactg gaggatatcg
     2161 tgagctttgc gggaatccgg aattgtggcg tcccggccgt cctggccgtg atgattttta
     2221 cgcttctggc gccgatggcg gccatggctt cagggggctc ggcgccttac cacgtgcccg
     2281 tgtatgcggt ggtgcctttt gtccttcttc ttctgtgcat cgcgattatg ccgctgaagt
     2341 tcccgcattt ctgggaaagc aacgcgaaaa agggaatcgt cgtggcgatc ctgtcggtgc
     2401 cgatagtcgg gttctatctt taccagaatg atgttcaccg gctgctggaa caccttcacg
     2461 aatacctgtc gttcatcgtc ctgctctggt cgctttacac catttcgggc gggatcgttc
     2521 tgcgcggcaa cctgaaggca agtcccgcgg tcaacaccgc ctttcttggg atcggagccc
     2581 tgattgcaaa ccttttcggg acgaccggcg cgtcgatgct tctgatacgc ccgctgctgc
     2641 gtaccaacca ggaacgcgag ttcaaggttc ataccgtcgt attcttcatc ttcgtcgtgt
     2701 cgaacatcgg cggctgcctg actccgctgg gcgatccgcc cctgtacatg ggattcatgc
     2761 gcggcgttcc gttccagtgg accctgtccc tgtggccgca gtggttgctg gtcaattcgc
     2821 tgctgctggt catttattac atgtgggatc ggattcggta ccggcaggaa aaggccgaga
     2881 acgtggccat ggacatgacc aacgccgaac cgctgcggat tgacggaaag atcaatatcg
     2941 ccctgatcct tggcgttgtc gcgacgatcg cgctttccgg cgtcctggaa atgaatccgt
     3001 ggctgcgcga cggcatcctg gttgccctcg gcctgctttc gatggcaacc acgaagaaga
     3061 agtaccggga agacaacgaa ttcaatttcg gtgcgatcac cgaggtcgcc gtgctgttca
     3121 tcggcatttt catcacaatg atcccggccc tgatgatgct cagcgaagcc ggtcccggtc
     3181 ttggcgtcaa tcacggatgg cagtacttct ggataaccgg cactctgtct tcgttccttg
     3241 acaacacccc gacctatatc gttttccttg accttgccca gtcaaccctg ggaagttcga
     3301 ccatcgaagc attcatgagt cacgtcgagg gtccggcgat attgaaggcg atttcgctgg
     3361 gggccgtgtt catgggcgcc aacacttata tcggaaacgg cccgaacttc atggtgaagg
     3421 caatcgcgga agcaaagggc agctcgcagg tcaagatgcc gtcgtttggc ggttacatgg
     3481 tctattcgat gttgattctt gtgccgatat tcctgctggt caccctgctg acgttcgttc
     3541 ttggcatcat gtagtcccgc ttccgtcggc ggcgttaccc cgcgcgtcca attcgcttcc
     3601 ggccatcgcc tccgtggaaa tgcgttccgc ctcatccgcc ctttcctgct cgtcgtcctc
     3661 tggcggagga atgacatcgg cgtttccgga accggccgaa agatcggcaa ggcgcctgtt
     3721 gtacgcaatg agaacgtcgg acgccgtcag tattccggca acgctgtcgg ttccgtcgtc
     3781 ggaaatcaca acgagttcct cggatccgcg tgatgtcatc ctgcggtgtg cggtcatcag
     3841 cgagtcggac agcctgacgc ttgcccatcc tggtgaatcc gccaccgggt gcatcaggtc
     3901 ggccgccacc agtccgcgcg cgcgttcacc ggcttcgtgc actgcctgga tcagctcact
     3961 ttgctggaaa acgccaagca atcgaccgtc ctgggatgtc accggataca cgcgctgtct
     4021 tgtgtccgcg gtccgctgca tgacctgggc gggggttgcg tcgcgcgcga tgcaggtgaa
     4081 tcggcggttc tttttgaaca cgtcgccgac ctttgacgag cccatgatct caaaggcgta
     4141 ttcgccgatg tgcgccggag agttgattcg cgacggcacc tggcttctga aaatggtcca
     4201 gcgccggctg accaggaagg atatcgagca cacccagacg gcagggacga tcaggtcgta
     4261 ccccccggtc atttcactga ccaggatcac cgctgaaagt ggtgtgttgg ccgctgccgc
     4321 gaagaatccc gccatcccga cgaccgcgaa cgttgaagga ttcggcacca gggtcggcca
     4381 ccaggaatgg agcaccattc cggccgatcc gccaagcgtg ccgccgatga ccatggacgg
     4441 gccgaacacg ccggcgctgc ctccggaacc gatcgtaagg gaagttgtaa gaatcttgcc
     4501 tgtcgcgacc acaagaagga ttaacgccgc catgcgcgaa ggttcacccg aaagggcatc
     4561 ctgaaggacc ccgtaccctg tcgaaaggac gtccatcact cggtcgtcgg gcgaaagctc
     4621 gataagggca agcgcaacgc cgccggtcag aaggccgcca attgcaggct taagaaggaa
     4681 tggaatgcgg atcttcctga aaaggccctc gattccatag aaggtccaga tgaacacaag
     4741 cgccccgg
//
LOCUS       DAOPPA010000242         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19652_length_4738_cov_3.663037, whole genome shotgun sequence.
ACCESSION   DAOPPA010000242 DAOPPA010000000
VERSION     DAOPPA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19652_length_4738_cov_3.663037"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKH54_08220"
     CDS             complement(<1..900)
                     /locus_tag="PKH54_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC99917.1"
                     /translation="MVLSQFLKRSGVLTFAFVAVACCACGSAEKGLEDVPEGDAVATD
                     SAPDVASDAVSDTASDAVSDTVSDVADDAHADDASADTAVDAAQPTDKPFRVAMVSDC
                     HVLPLGLDKKPNKFIVELLQNISNFAEPPAAVFALGDNIEDMFVWYEEAQAGLPIPVL
                     DVFHQIFADSLSVPWYLALGNHDVRFVDTFHELEVPISRWLQYFEGTQHLPARWYSVK
                     MGGFNFVVLFSSDGAIDHETNDTNILLQEQIDWLNGELALGMPTVLLYHIWIEPPLES
                     PEADAETELHPIFQAVIDHPGVVK"
     gene            complement(905..1039)
                     /locus_tag="PKH54_08225"
     CDS             complement(905..1039)
                     /locus_tag="PKH54_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99918.1"
                     /translation="MNTFASRAIIMTVRECRTRVHRMKAGFGFDMSVSDEPVVCIDKE
                     "
     gene            1233..3908
                     /locus_tag="PKH54_08230"
     CDS             1233..3908
                     /locus_tag="PKH54_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOC99919.1"
                     /translation="MKSGYGRSTRRVVKLLCTALILAESSVWAMGVSADTTADRIAVP
                     TTAPTPQVAPDPAAGTRLVRFGVYQNRPKVFLDEHGIGAGFFAEMLGELAVRNGWLIS
                     EVPCDWNDCLQALEDGRLDLMPDVAWSPDRAARFDFHTIPVLESWSQIYCRNGRYPQT
                     LEDLAGMRVAVLQGSIQHDSFKELSSGFGINVEIIPTNTLDDAFVAVNDGRADAAIAN
                     RFFGDYFFASYGLTKTPVVFQPVNLYFATGRGRNADLLRVIDDTVVSQRRTPNSPYYT
                     MLSKWMTGPTVSKIPPVVLWIIGGILFLLGVFIVAATILRSQVRSKTAWLAHANDELR
                     DLSQALTKSQSLMKLTQEIALVGGWEWDVQQRRMTWTENACQDMGFQILMSGDGSPLH
                     HRNGCSSWTSNDESGHPPGGGSVSMSDPEGKSHSEGASPCPFAAADIERVSAAFVRCI
                     ADGTPYDLEAGFAAPGQPAMELRTSARAVRDESGNIVRIIGIFMDITRLKDEEAARTR
                     AEEELRIARRMETIGHLTGGIAHDFNNLLSAILGYSGLILRRDDTSEAVRRDMAQIQA
                     AGERAANLSHQLLAFGSRKISQPRPISMNSVISGLERMLLRLLGENIVIDVRLAADAG
                     SVMADQGQMEQILINLAVNARDAMPDGGRLTISTSSLILGASRSMDRIELPAGRWVVV
                     TVSDTGIGMDQATRARIFEPFFTTKRLGSGSGLGLATVFAIINQSGGHIQVETAPGQG
                     AAFSIFLPGLDIPAAPQAIQDESRITPGSATILLVEDDETVRLVIERILVEAGYRVLT
                     AADGQEALGVCTLHSREIELILTDIIMPRLSGDELATTVQMRHPRIPILFMSGYADHR
                     VADNPDLNPETNFISKPFGAFELTERIHRILSRQD"
     gene            4224..4679
                     /locus_tag="PKH54_08235"
     CDS             4224..4679
                     /locus_tag="PKH54_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99920.1"
                     /translation="MATAKPGKATKTQIIPCRPDKHNCLMMEVASWEQSPTLVIRQRG
                     KGTQGELYRKDGKRQTVGQTSEFGGAYQELKLASWQEIDDICNDNYNCSSELRPLLAR
                     LKTLFPTGLPLIQAEDNNFSGECCPYQISALVYLDGAALKFALLAKSQD"
BASE COUNT         1075 a         1419 c         1280 g          964 t
ORIGIN      
        1 cttgacgacg ccggggtgat caatgacagc ctgaaaaatc gggtggagtt ccgtttcggc
       61 atcggcttcg ggagattcca ggggcggctc gatccagatg tgataaagaa ggactgtcgg
      121 catgcccagt gccagttcgc cgttcagcca gtcaatctgt tcctgaagca ggatatttgt
      181 gtcgttcgtc tcatggtcaa tggccccgtc cgagctgaac agcacgacaa aattgaagcc
      241 acccatcttc acggagtacc agcgggcagg caagtgctgc gtgccttcga aatactgaag
      301 ccatctggaa atcggcactt ccagttcgtg gaaggtatcg acgaaccgga cgtcatggtt
      361 tcccaacgcc agataccatg gcacggacag gctatcggca aagatctgat gaaaaacatc
      421 aaggaccggt atgggcaaac ccgcctgggc ctcttcgtac cagacaaaca tgtcctcgat
      481 gttgtctcca agcgcgaaaa cggcggcggg cggttccgcg aaattcgaga tgttttgcag
      541 cagttcgaca atgaatttgt tcggcttttt gtccaaccca agtggcagca cgtgacagtc
      601 cgacaccatc gccacacgga atggcttatc agtgggctgc gcagcatcga cggccgtgtc
      661 ggcactggcg tcgtcggcat gggcatcatc ggcaacatcg ctgaccgtat ccgacacagc
      721 atccgacgcc gtatccgaca cagcatccga cgccacatcc ggtgccgaat cagtcgccac
      781 ggcatcccct tccggcacat cctcgagccc cttttccgcc gagccgcatg cgcaacaggc
      841 aacggcaaca aacgcgaacg tcagaacccc ggaacgcttc aggaactggg aaagcaccat
      901 tgaatcactc cttgtcgatg cagacgactg gttcatcaga cacggacata tcgaacccga
      961 atccagcctt catgcgatga acccgagtgc ggcattcccg aaccgtcatg atgattgccc
     1021 gcgaggcaaa agtgttcaag acaaaattcc ccgccaccac gaatccatcg tggctcgcgc
     1081 gggggcgacc gagttcagat tgacggagtc gtcgatctgg aatagcgttg attccagtta
     1141 aagaaacgac cggcccttca tctcactgaa gctgtcgcct ttcccgggcg atggaatcgg
     1201 gacttgcagg ggccggcggg gattgaaagc acttgaaatc cggatacggc agatcgaccc
     1261 ggcgtgtcgt caagctcctt tgcaccgcgc tgattctggc ggaatcatcc gtctgggcca
     1321 tgggcgtcag cgccgacacg acagccgacc gaatcgccgt tccgaccacc gccccaacgc
     1381 cccaggtggc gccagacccc gcggcaggaa cgcgccttgt acgctttggc gtatatcaga
     1441 atcgtccaaa ggtctttctg gacgaacatg gcatcggagc cggctttttc gccgaaatgc
     1501 ttggcgaact cgcggtccgc aacggttggt taatcagcga agtcccatgc gactggaacg
     1561 actgtctgca ggcccttgaa gacgggcgcc tcgacctgat gccggacgtg gcctggtccc
     1621 cggaccgcgc tgcccgtttc gattttcaca ccatccccgt ccttgaaagc tggtcccaaa
     1681 tttactgccg gaacggtcgc tatccacaga ccctggagga tcttgccggc atgcgcgttg
     1741 ccgtccttca aggctcaatc cagcatgact cattcaagga gctttccagc ggcttcggaa
     1801 tcaacgtgga gattatcccg accaacacgc ttgacgacgc ctttgtcgcg gtcaacgatg
     1861 gacgggccga tgctgccatc gccaatagat tctttggcga ctacttcttc gcctcatatg
     1921 gccttaccaa gactccggtc gtcttccagc ccgttaacct gtacttcgcc accggccgcg
     1981 gaagaaacgc cgaccttttg cgggttatcg acgatacagt ggtctcccag cgccgtaccc
     2041 cgaattcacc gtactacaca atgttgtcca agtggatgac cgggccaacc gtttcgaaaa
     2101 tccccccggt ggttctgtgg atcatcggcg gcatcctgtt cctgctgggc gtgttcattg
     2161 tcgcggccac catcctgcgc agccaggttc ggtcgaagac ggcctggctg gcccatgcaa
     2221 acgatgagct tcgtgattta tcccaggcac ttaccaagag ccagtccctt atgaaactta
     2281 cccaggaaat cgccctggtc ggcggatggg aatgggatgt acaacaacgc aggatgacgt
     2341 ggactgaaaa cgcctgccag gatatgggtt tccagattct gatgtcgggg gatggttcgc
     2401 cactgcacca cagaaacggt tgttcatcgt ggacttccaa cgatgaatcc gggcaccctc
     2461 cgggcggcgg gtcagtttcg atgtccgatc cagaaggcaa atcccattca gaaggtgcgt
     2521 cgccctgccc gttcgccgcc gccgatattg agcgcgtgtc tgcggccttt gtccgttgca
     2581 ttgccgatgg aacgccctat gaccttgaag ctgggttcgc cgcgccaggc cagcccgcca
     2641 tggaactcag gacatcggcc cgcgcagtgc gcgatgaatc gggaaacatc gtaagaataa
     2701 tcggcatctt catggacatt acccgcctga aggacgagga agccgcccgc acaagggccg
     2761 aggaagaact tcgcatagcg cgcaggatgg aaacaattgg ccacctgacc ggcggaatcg
     2821 cacatgattt caacaacctg ttaagcgcca tccttgggta ctccggacta atccttcgtc
     2881 gcgatgatac cagcgaggcg gttcggcggg acatggccca gatccaggct gccggagagc
     2941 gggctgccaa cctgtcgcac cagttgcttg cattcggatc acgaaaaatc tcgcagccgc
     3001 gaccgatttc catgaattcc gtgatatccg gcctggaacg aatgctcctg cgacttcttg
     3061 gggaaaacat tgtcatcgac gttcgtctgg cagcggacgc cggctcggtc atggccgacc
     3121 agggtcagat ggaacagatc ctcataaacc ttgccgtgaa cgcgcgcgac gccatgccgg
     3181 atggtggccg actcacaatc tcgacatcat cactgattct tggcgcatcg cgatccatgg
     3241 atcgcatcga acttcccgcc ggccgctggg ttgtcgtcac ggtctccgat accggaatcg
     3301 gcatggatca ggcaacccgg gcaaggatct ttgaaccgtt cttcacgacg aagcgtctcg
     3361 gcagtggttc gggactggga cttgcaacgg tattcgcaat catcaatcag tcgggtggcc
     3421 acatccaggt cgaaacggcc ccgggacaag gtgcggcatt ctcaatcttc cttcccggcc
     3481 ttgatatccc ggcggcgccg caggcaattc aggacgagtc acgaatcaca ccagggagtg
     3541 caaccatctt gctggtcgaa gatgacgaaa ccgtgcggct ggtcattgaa cgtatcctgg
     3601 tcgaggccgg ctaccgggtt ctgaccgccg cggacggcca ggaagccctt ggtgtctgta
     3661 cacttcattc ccgggaaatc gaactgatcc tgaccgacat catcatgccc cgcctgtccg
     3721 gcgacgaact cgcgacaacg gttcagatga gacacccccg cattccaatc cttttcatgt
     3781 ccggctatgc cgaccaccgc gtcgccgaca accccgacct taaccccgaa accaatttca
     3841 tttcaaaacc tttcggcgcc tttgaactga cggaacgtat ccaccgaatc ctttccagac
     3901 aggactgacg cccgccatgg tgccacccag accctgtacc tccatccagt tgccaaggta
     3961 cctcaggtac tgactgatca tttcgacgtg gccgcggtcg aggccggcct tcaattcgtc
     4021 gatgcaccct agcaggcccg ggtcggtaat cggaccgctg ttgacattga catatgcgcc
     4081 ccctaacatc gaaccaaaca ctccatggca atacaattat gaaagcttcc cgacggcagg
     4141 atgaacatcg gcaagttcga acaatctgga cgaaaaaggc tctattcgca tcaatggctt
     4201 gcgtaatgct gctggggaca ggcttggcaa ccgccaagcc ggggaaagcg accaaaaccc
     4261 aaatcattcc gtgtcgcccg gacaagcaca actgcctcat gatggaagtt gcatcatggg
     4321 agcagagtcc aacccttgtc atccgtcaaa gagggaaagg gactcaaggg gaattgtacc
     4381 gaaaggatgg caagcgacag accgttgggc aaacttctga gtttggaggg gcatatcaag
     4441 aattgaagct tgcatcctgg caagaaattg atgacatctg taatgacaac tacaactgca
     4501 gcagtgagtt gcgccctcta ttggcaagac tgaagacact ttttcccacc ggcctcccgc
     4561 ttatacaggc tgaagataac aacttctccg gcgaatgctg cccatatcaa atttcagcac
     4621 tcgtgtatct cgatggagca gctttgaagt ttgctttgtt agcaaaatct caggactagg
     4681 ggaaagcccg gatgcagatg tccttgcaga caggcaggca aattctggct attcgttt
//
LOCUS       DAOPPA010000243         4736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19664_length_4736_cov_4.007691, whole genome shotgun sequence.
ACCESSION   DAOPPA010000243 DAOPPA010000000
VERSION     DAOPPA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19664_length_4736_cov_4.007691"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /gene="rplI"
                     /locus_tag="PKH54_08240"
     CDS             complement(<1..233)
                     /gene="rplI"
                     /locus_tag="PKH54_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOC99921.1"
                     /translation="MKVILNEHMEHLGKAGEIVDVADGYARNYLIPQGKAVLATTRSV
                     KTLEHAKRQVQAQLLRERKTAEAMKAVIEKTSV"
     gene            complement(320..589)
                     /gene="rpsR"
                     /locus_tag="PKH54_08245"
     CDS             complement(320..589)
                     /gene="rpsR"
                     /locus_tag="PKH54_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOC99922.1"
                     /translation="MMKSATSGNRDKKKRRGPPRRKVCPFCQDKGSKIDYKNPAALKR
                     LVTERGKIVPRRISGVCARHQREVSLAIKRARHIALMPYSAKAMP"
     gene            complement(586..1089)
                     /gene="rpsF"
                     /locus_tag="PKH54_08250"
     CDS             complement(586..1089)
                     /gene="rpsF"
                     /locus_tag="PKH54_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOC99923.1"
                     /translation="MSTVLLRKYESVLVVRSDIGQDAMRRLHEKFLELLEKHNGRLVR
                     FEVWGKRRLAFTIRKCTKGVYLYYVYLAGEDFVEELSRNLRINTNVLRYMSIVLETEI
                     DPATFDFAAEEHIDKLPEQDEHGDRNRSTTGWDAEAAAEMAADMSDDDEEEEDIDDED
                     DGEEDER"
     gene            complement(1089..1724)
                     /gene="pth"
                     /locus_tag="PKH54_08255"
     CDS             complement(1089..1724)
                     /gene="pth"
                     /locus_tag="PKH54_08255"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOC99924.1"
                     /translation="MKLVCGLGNPGARYAGTRHNAGFMAVEAFCDKLNPSWSEKWESR
                     IARVEFQGVQILVQEPLTFMNLSGFALVRAAGFFRIAVDDVIVVHDDIDLPIGRVQVK
                     FGGGDGGHKGIRSIIEQLGSGDFARIRVGVGRPAGRGPDVVDFVLQPFSADEEKELSE
                     AIDVVSVAIREWVVGGVPKAQNKVNRRGNPKRGEPSCPPERSDGPKDREEV"
     gene            complement(1754..2377)
                     /locus_tag="PKH54_08260"
     CDS             complement(1754..2377)
                     /locus_tag="PKH54_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOC99925.1"
                     /translation="MEYPVIRAKDRAIVGTGIARKVRELGFIPAVVYGGNGESRSLVV
                     DPKVVGDILRSAGGRNKIVSLSIEGDKPEQTLAIVRDFQLETIKRTLLHCDFLRVSEE
                     SPVLVKVPVRIFGKSEAEKLGGVVKQTMRFVTIRCPAVRIPEEIGIDASKLNMGDELK
                     ISQLPLPEGARPVFLRDNKVILVSIPKAEKTDEAAPAEGEAAAPAAQ"
     gene            complement(2427..3377)
                     /locus_tag="PKH54_08265"
     CDS             complement(2427..3377)
                     /locus_tag="PKH54_08265"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99926.1"
                     /translation="MNCPAKINLGLSVLGMRPDGYHDLSGIMVPVPWHDRLDVEIDAA
                     LPAGRIRLEVVTQPGVSIGPIPEDDGNIVSATARLCLDCDEMAERPFGGGLFVRLFKA
                     IPAGTGLGGASSDAAGTILAIDRLARAAGHPGISPETMRAIAAAVGSDSVFFIRPVPS
                     RIVGRGEDFVPVPLAGSFSAVIAVPVQRLNTGAVFRRWEELRKHSDSGKDGLTDRVVG
                     DISPCLLVRVPPGSGEPAAPEFFENDLSEAVFDLCPRSAKLLDLFNESGSFAASVTGS
                     GSAVYGLFADIGSAECFVRTMTRNLLPGEIVRAFQIGSDF"
     gene            complement(3361..4011)
                     /locus_tag="PKH54_08270"
     CDS             complement(3361..4011)
                     /locus_tag="PKH54_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC99927.1"
                     /translation="MRGPGRFKERFDGFVFDLDGTLVDTFVDIQTAINLVRRDLSLPP
                     LDLETVTLCVGNGARQLVRKTIGDLDAGEEARLVGLFRTYYRQHLHDCSRLYPNVIST
                     LDSLSGHKLAVLSNKPHEPCVELLRALGILDRFLIVRGQDDRWPSKPDPQSLLFIVRD
                     VFQTVPERTLMVGDWDTDVFTARNAGMPCAIVRTGMSRFMGAVPDWYLDDIVELSR"
     gene            complement(4011..>4736)
                     /locus_tag="PKH54_08275"
     CDS             complement(4011..>4736)
                     /locus_tag="PKH54_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC99928.1"
                     /translation="DGDDDIVKLINALQHVDGLEWVRLMYLYPDDVTDGLIDAVAESV
                     NCLPYFDIPIQHGDSGVLARMNRGGDAGRILNLVRRIRDRIPGVVLRTTLMTGFPGET
                     PEAFDNMLALARAAEFDMVGVFQFSPEPGSAAARLPDQVPSDVAAARCRTLQDELSRI
                     AARNREGLVGRCFQAVAEHIDEDGRVVGRMWFQAPEVDGVTYVDGLSDDDETVMAVQV
                     TGFRDSDFFARVQAGPGFFGGNL"
BASE COUNT          955 a         1565 c         1312 g          904 t
ORIGIN      
        1 ttgacggaag tcttctcgat gactgccttc atcgcttccg cggtcttgcg ctcgcgaaga
       61 agctgtgcct gaacctggcg cttcgcgtgc tcgagggtct tcaccgaccg ggtcgtcgcc
      121 aggactgcct ttccctgcgg gatcaggtaa ttccgggcgt acccgtcggc gacatcgacg
      181 atctcgccag ccttgcccag gtgttccata tgctcgttaa gaatgacctt cattccttaa
      241 tctccatggc cggcgccttc gacaccgacc tcaaccggcc cgggcccgac cattcggtca
      301 cgacccgtgc ctgcccgggt cacggcatcg ccttggcgct gtacggcatc agggcgatgt
      361 gcctggcgcg cttgattgcc agcgagactt cccgctgatg cctggcgcag acgccgctga
      421 tgcggcgcgg aacgatcttg ccccgctccg tgaccagcct cttcaaagct gccgggttct
      481 tgtagtcaat cttcgagccc ttgtcctggc agaacgggca aaccttgcgc cgcggcggtc
      541 cacgacgctt cttcttatca cgatttccgg aggttgcgct cttcatcatc gttcatcctc
      601 ctcgccatcg tcctcgtcgt cgatgtcttc ctcttcctcg tcatcatcgc tcatgtcggc
      661 agccatctcc gccgccgcct cggcatccca gccggtcgtc gaacggttgc ggtcgccgtg
      721 ttcatcctgc tcggggagct tgtcgatgtg ttcctcggcc gcaaaatcga aagttgcggg
      781 atcgatttcg gtttccagga caatggacat gtagcgcagg acgttcgtgt tgatacgaag
      841 gttccggctc aactcctcga cgaaatcctc gccggccaga tagacgtagt aaaggtacac
      901 gcccttggtg cacttccgga tggtaaacgc caggcggcgc ttgccccaca cctcgaacct
      961 gaccagcctg ccattgtgct tctcgagcag ttcaaggaac ttctcgtgaa ggcgacgcat
     1021 ggcatcctgt ccgatgtcgc tccttacgac caacacggac tcgtacttgc gcagcagaac
     1081 cgtactcatc aaacctcctc acggtctttc ggcccgtcgg acctttccgg cgggcaagaa
     1141 ggctcacccc gcttcgggtt tccccggcgg ttcactttat tctgggcctt cgggacaccc
     1201 ccgacgaccc attccctgat tgccacggaa acgacgtcaa tcgcctccga caattccttt
     1261 tcctcgtcgg ccgaaaacgg ctgcaggaca aaatcgacca catccggtcc acggcctgca
     1321 ggccttccca ctccgacgcg aatccttgcg aaatccccgg acccgagctg ctcgataatc
     1381 gagcggatgc ccttgtgccc gccgtcgccg ccgccgaact tcacctgaac ccggccaatc
     1441 ggcagatcga tgtcgtcatg aacgacgatc acgtcatcga ccgcgatccg gaaaaacccg
     1501 gcggcgcgta caagcgcgaa gcctgaaagg ttcataaagg tcaacggttc ctggaccagg
     1561 atctggacac cctggaattc aacccgtgca atccgggatt cccatttctc ggaccaggaa
     1621 ggattcaatt tgtcacagaa cgcctcgacc gccatgaaac cggcgttatg cctggttccg
     1681 gcatacctcg cgccgggatt gcccaacccg catacaagct tcacagacag cctcgatggc
     1741 gggcacctgc cgcttactgg gcagccggag ccgcggcctc accctcggcg ggtgctgctt
     1801 catcggtctt ctcggccttc gggatggaaa caaggatgac cttgttgtcg cgaaggaaga
     1861 ccggcctggc gccttcggga agcggaagct ggctgatctt cagctcgtcg cccatgttga
     1921 gcttcgacgc gtcgatgccg atttcttccg gaatcctgac cgccggacac cttatcgtta
     1981 caaagcgcat ggtctgcttc acgacgccgc cgagcttttc ggcctcgctc tttccgaaaa
     2041 tcctgaccgg aaccttgacc aggaccggcg attcctcgga aacgcgcagg aagtcgcaat
     2101 gcagaagcgt gcgcttgatg gtctcaagct ggaaatcacg gacgatcgca agggtctgct
     2161 cgggcttgtc gccctcgatt gaaaggctga caatcttgtt ccggccacct gccgaacgca
     2221 ggatgtcgcc gacgaccttc gggtcaacca ccagactgcg gctttcgccg ttgccgccat
     2281 aaacgacggc aggaatgaat ccgagttccc tgaccttgcg tgcgattccg gttccgacga
     2341 tggccctgtc cttggccctg ataaccgggt attccatgat aacctccata aggtcgcgcc
     2401 ctgtcgtcgc aggtaacgcg cagacctcag aaatccgatc cgatctgaaa tgcacgaaca
     2461 atctcgccgg gaagcaggtt cctggtcatc gtgcggacga aacattccgc cgacccgatg
     2521 tcggcgaaaa gcccgtacac cgcggaaccc gaaccggtca cggaggcggc aaaagagccg
     2581 gattcattaa acaaatcaag caacttcgcc gaacgcggac aaagatcgaa caccgcttca
     2641 gacagatcat tttcaaaaaa ctctggtgcc gccggctcgc ccgacccggg cggtacgcgc
     2701 accaaaaggc aggggcttat atcaccaacg actctgtcag tcaagccatc cttcccggaa
     2761 tcactatgct tccgcagttc ctcccatcga cggaaaaccg cgcccgtatt cagtctctgc
     2821 accggaaccg caatgacggc cgaaaaactc ccggccagcg gaaccgggac gaaatcctcg
     2881 ccccgaccga caatccgcga aggaaccggc ctgatgaaga acacgctgtc ggaaccgacc
     2941 gccgccgcta tcgcccgcat ggtctcgggc gaaatgcccg gatgcccggc ggcccgggcc
     3001 agacggtcga tggcgaggat ggttcccgcg gcgtcggatg acgcgccccc aagaccggtt
     3061 cccgctggaa tcgccttgaa aaggcgcacg aagaggccgc ccccgaaggg acgctccgcc
     3121 atctcatcgc agtccaggca cagcctggcg gtcgccgaaa cgatattgcc gtcatcctcc
     3181 ggaatgggcc cgatgctaac ccctggctgc gtgacgactt ccagacgaat ccggccggcc
     3241 ggaagcgcgg catcgatttc gacatcaagc cggtcatgcc agggcacggg caccattatg
     3301 ccggagaggt cgtgataccc gtcaggacgc attccaagaa cggaaaggcc caggttaatt
     3361 ttagcgggac agttcaacga tatcatcgag gtaccagtcg ggaaccgcgc ccatgaagcg
     3421 ggacattccg gtccttacga ttgcacacgg catcccggcg ttccttgccg tgaaaacgtc
     3481 ggtatcccag tcaccaacca tcagggttct ttccggaacc gtctggaaaa catccctgac
     3541 gatgaaaagc aggctttgcg ggtcgggctt ggacggccac ctgtcgtcct gtccccggac
     3601 gatcaggaaa cggtcaagta tgccaagcgc ccgcagcagt tcgacacacg gttcgtgcgg
     3661 cttgtttgaa aggactgcaa gcttgtgacc cgacagggaa tcgagcgtgg aaatcacgtt
     3721 cggatacaac cggctgcagt cgtgcaggtg ctgacggtag tatgtccgaa aaaggccgac
     3781 aagccgcgct tcctcgcccg catcaaggtc gcctatggtc tttcgcacaa gttgccgtgc
     3841 tccgtttccc acgcacaggg tcacggtttc aagatccagc ggcggcaacg acaggtcgcg
     3901 gcgcacaagg ttgattgcag tctggatgtc gacaaacgta tccacaaggg ttccgtcgag
     3961 gtcgaaaacg aaaccgtcga aacgctcttt gaagcggcct ggcccacgca tcagaggttc
     4021 cccccgaaga agcccggccc ggcctgaacc ctggcgaaaa agtccgaatc ccggaatccg
     4081 gtaacctgca ccgccatgac cgtctcgtcg tcgtcggaaa ggccgtcgac atacgtcacc
     4141 ccatcgactt ccggagcctg gaaccacatc cggcccacga cgcggccgtc ttcatcgata
     4201 tgctccgcca ccgcctggaa gcacctgccg acgagcccct ccctgttccg ggcggcgatc
     4261 ctcgaaagtt cgtcctgaag tgtccggcat cgcgcggcgg cgacatcgga tggaacctgg
     4321 tcgggaaggc gggccgccgc ggatccgggc tcgggtgaga actggaacac cccgaccatg
     4381 tcgaactcgg cggctctcgc aagggcaagc atgttgtcaa acgcctcggg agtctcgccc
     4441 gggaatcctg tcatcagggt tgtgcgaagc accacgcccg gaatcctgtc acgaatcctg
     4501 cgcaccagat tcagtatccg gccggcatcg ccgccccggt tcatcctggc cagcacgccc
     4561 gagtccccat gctgaatcgg tatgtcgaaa tacggcaggc agttgaccga ttccgcgaca
     4621 gcgtcgatca ggccgtccgt gacgtcgtcg ggatacaggt acatcagccg tacccattcc
     4681 aggccatcga catgctggag ggcgttgatc agcttcacga tatcgtcgtc gccgtc
//
LOCUS       DAOPPA010000244         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19795_length_4709_cov_4.579287, whole genome shotgun sequence.
ACCESSION   DAOPPA010000244 DAOPPA010000000
VERSION     DAOPPA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19795_length_4709_cov_4.579287"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="serS"
                     /locus_tag="PKH54_08280"
     CDS             complement(<1..982)
                     /gene="serS"
                     /locus_tag="PKH54_08280"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOC99929.1"
                     /translation="MHDVKYLLENYDLAATRLESRGYTDLGWLAELAEKRRVMVGQRD
                     GLRGDQKKVSDSFKLPGTTPEMREELRERSKAIGLQIDAMTAELEAIEADLENRLMYV
                     PNLPDELTPVGPDESGNVVARAWGTPRQLPFKALEHWELGEKLNILDFERARKISGAR
                     YVIYRGAGARLERALAAFMLDIHTTEHGYTEILPPFLVLEQCMRGTGQLPKFREEAYV
                     ATDEMFLIPTAEVPVTNMHREEILDAKDLPLKYAAYSACFRREAGSYGRDVKGMTRVH
                     QFQKVELVKFTTPETSEEEHQKLTADAERIFQRLELPYPVMELCSGDIGFS"
     gene            complement(1029..3575)
                     /locus_tag="PKH54_08285"
     CDS             complement(1029..3575)
                     /locus_tag="PKH54_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOC99930.1"
                     /translation="MEFELARKIVISADSYEARAALLDGDLLINLEIESAGSNRKKGN
                     IYRGRVTTIEKGLDAAFVDYGTGKEGFLPLDEVNERSLIELTGKSVAANGHVSLGVGD
                     TVLVQVAKEEVGKKGAALTMNISSPGRFLVLMPFSSRTGVSRRLAGDDRTRLKDALDR
                     LSLPEGFGLIIRTASDQSMEKELQADLNHLLSQWKDILSRFNQAKKPTEVHTESSLAL
                     RFIRDYMDAGVSEVIVEDQGTWQELNGYFASVMPGWQSILSRYTGDLPLFVKFNVDRQ
                     VEALLKNKVILPSGGSIVIGQTEALTAIDVNSGRTKKGAIEDTAFKTNLEAAVEIARQ
                     VILRDIGGIIVVDFIDMESEAHRKGVEDALRNALARDKAHLTVARIRDFGLLAFSRQR
                     LRHTVEEGIMSACPACGGTGRIRAPGPLAMSIMRRLRERLANMTSEAELVEVTVSVDV
                     ANFLNNRKRDELIALEKKHGLAIEILGTTDVAAEDMQIRIHEDIPDDRQLARARIEGH
                     EEPAAAAPAATKDAQQPAVAAGQPASPPKQKPSLLKGLIAKLLDLDEFDEVPTRGKGK
                     GETQPPATAKPTVSTTSGRDPISTASPSGSSAGAIPSAATEVNAPATGPQAGNRRNQS
                     ERRKDDSTAGADAEDNQQAKPREGRSHRSRSSRGRGQKPAQQQDQSATAQDPSTAPAR
                     DGRKASDAKRQPAPAADVTRATSTTASGNGGQSRQGQVTPDGSTAPAVDGEQPARSKR
                     SRSRRRRSSQKSPADQGTATTTLPTGSSQAGAAPTSAGSVTAPVVNTLASVGEGSRSP
                     AERRESDGGDAQGAPDKPKAPRKRYRSRKPKTGNAEGRGPNPPDGGQGQK"
     gene            complement(3598..4356)
                     /locus_tag="PKH54_08290"
     CDS             complement(3598..4356)
                     /locus_tag="PKH54_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC99931.1"
                     /translation="MTQPAPKDGISIVIPSYNESSRIGATLSAIRAYAERNLADWEII
                     VVDDGSSDNTADVARSTIPDSQRLVVVSNPGNRGKGFSTKNGCAHARFPYVLMSDADL
                     STPIEELEKLAVHAAPDTVVIASRGMAGSNLEVRQPLYRELMGRVFNLMVRIFVIGGI
                     SDTQCGFKLFGPEVVRNLMPLLQTDRFAFDVEILARARQMGYPIHEVPVRWRNDDRTR
                     VSALSDSAGMFMDMLAVRRMVNRSPGPAKPGSDN"
     gene            complement(4356..>4709)
                     /locus_tag="PKH54_08295"
     CDS             complement(4356..>4709)
                     /locus_tag="PKH54_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC99932.1"
                     /translation="EAAGLNAEDYRLYASPDETCPAGTVTKILAGGADGQVYPVGRRA
                     VVIGREVADVSCPEDRYMSRVHASVEPVSSGLALKDKQSRNGTYIKTSGSITVRDGDV
                     ILVGRQLLKVEAKPQ"
BASE COUNT          797 a         1468 c         1429 g         1015 t
ORIGIN      
        1 ccgagaaccc gatgtcgccg ctgcacagtt ccatcactgg atagggcagt tccaggcgct
       61 gaaagatgcg ttcggcgtcc gctgtcagct tctggtgctc ttcctcggat gtttccggtg
      121 tggtgaactt gaccagttcg accttctgga actgatgcac gcgggtcatt ccctttacat
      181 cccggccata gctgccggcc tcgcgacgga agcaggccga atacgcggcg tatttcagcg
      241 gaagatcctt tgcgtccagg atttcctcgc ggtgcatgtt ggtgactgga acctcggcgg
      301 tcgggatcag gaacatctcg tcggtggcga catacgcttc ctcccggaac ttgggaagct
      361 gtccggtccc ccgcatgcac tgttcaagga caaggaacgg cggcagaatt tcggtatagc
      421 cgtgttccgt cgtgtggatg tccagcatga aagccgccag ggcacgctcc aggcgggcac
      481 cggcgccgcg ataaatcaca tatctggcac ccgagatctt gcgggcgcgc tcgaagtcca
      541 ggatgttcag cttctcgccc agttcccagt gctcaagcgc cttgaacgga agctggcgcg
      601 gcgttcccca tgcgcgggca acgacgtttc cggattcgtc gggcccaaca ggggtgagct
      661 cgtccggcag gtttggaacg tacatcagcc ggttttcaag gtcggcctcg atcgcctcaa
      721 gttcggccgt catggcgtca atctgcaagc cgatggcctt gcttctttcc cgcagttcct
      781 cacgcatttc cggcgtggtg ccgggcagtt tgaatgaatc cgagaccttc ttctggtcgc
      841 cgcgaagtcc gtcgcgctgg ccgaccatca cgcgacgctt ttcggcaagt tcagccagcc
      901 atccaagatc cgtgtatccg cgtgattcca gccgcgtcgc ggccagatca taattttcaa
      961 gaaggtattt gacatcgtgc atgattgact ccttttaccg ttcgtcggga atttccggcg
     1021 gatacttact acttctgacc ctgcccaccg tccggcggat tcggtccgcg accttcagcg
     1081 ttgccggttt tcggtttgcg ggaacggtac ctcttccttg gggccttcgg cttgtccggg
     1141 gcgccctggg cgtccccgcc gtcggattcc ctgcgttcag cgggcgaacg tgaaccctcg
     1201 ccgaccgagg caagcgtgtt cacgactggc gccgtgaccg agccggcaga ggtcggggca
     1261 gccccagcct gtgacgagcc ggtcggcagc gtggtggtcg ccgtgccctg atcggccggg
     1321 ctcttctgac ttgatcgccg cctgcggctg cgcgagcgct tcgacctggc aggctgttcg
     1381 ccgtcaaccg ccggagcagt tgaaccgtcg ggcgtcacct ggccctgtcg cgactgaccg
     1441 ccgttgcctg aagccgttgt cgatgtcgcc ctggtgacgt ccgcggcggg ggcgggctgt
     1501 cgctttgcgt cgctggcctt tcggccatcg cgggcggggg cggtcgaagg atcctgcgcc
     1561 gttgccgact gatcctgctg ctgtgccggc ttctgtcccc ggccccttga cgatcttgat
     1621 cggtgggagc gcccctcccg gggcttggcc tgctggttgt cttccgcgtc tgccccggcc
     1681 gtggagtcat ccttgcgccg ctcggactgg tttcggcgat tccccgcctg cgggcccgtg
     1741 gctggagcat taacctctgt cgccgcggac gggatcgcgc cggccgagga tcccgatggc
     1801 gatgccgtgg aaatcgggtc acgtccggaa gttgtcgaaa ctgtcggctt cgcggtcgcg
     1861 ggcggctggg tctccccctt gcccttgccg cgtgtcggca cttcgtcgaa ttcgtcaaga
     1921 tcaagcagct tcgcgatcag gcccttcaga agcgacggct tctgttttgg cggtgaggct
     1981 ggctgcccgg cggcaacggc gggctgctgg gcatcctttg tcgctgccgg cgccgccgcc
     2041 gccggttcct cgtgaccctc tatccttgca cgcgcaagct ggcggtcgtc gggaatgtcc
     2101 tcgtgaatcc ttatctgcat gtcctcggcc gccacgtccg tggttccaag gatttcaatg
     2161 gccagcccgt gcttcttttc aagcgcaatc agttcgtcac gcttcctgtt gttcaggaaa
     2221 ttggcgacat caaccgaaac ggtcacttca accagctcgg cttcgcttgt catgttggca
     2281 aggcgctcgc gcagccggcg cataatcgac atcgcaagcg ggccgggtgc ccggatgcgt
     2341 ccggtgccgc cgcacgcggg gcaggccgac atgatgcctt cctcgacggt gtggcggagc
     2401 cgttgacgtg aaaaggccag caacccgaag tcccgaatcc tggcaacagt cagatgggcc
     2461 ttgtcgcggg ccagcgcgtt gcgaagagcg tcctcgacac ccttgcggtg cgcttccgac
     2521 tccatatcga taaaatcgac gacgataatc ccgccgatgt cgcgcagaat cacctggcgc
     2581 gcgatttcaa ccgcggcttc caggtttgtc ttgaaagccg tgtcctcgat ggcgcctttc
     2641 ttcgtccgcc cgctgttgac gtcaatcgcc gtcagtgcct cggtctggcc gatcacgatg
     2701 ctgccgcccg aaggcaggat aaccttgttc ttcagcagcg cctcgacctg gcgatcgaca
     2761 ttgaacttca cgaaaagtgg cagatcaccc gtgtatcttg aaaggatcga ctgccagccg
     2821 ggcattacgg aagcgaaata gccgttaagc tcctgccacg tgccctgatc ctcgacgatg
     2881 acctcgctga cgccggcatc catgtaatcg cggatgaaac gaagcgccag gcttgattcc
     2941 gtatgaacct cggtcggttt ctttgcctgg ttgaacctgg acaggatgtc cttccactgg
     3001 gaaagaaggt ggttaaggtc tgcctgcagt tccttttcca tcgactgatc gctggcggtt
     3061 ctgatgatca ggccgaaacc ttccggcagc gacagtcgat cgagcgcgtc cttcaggcgg
     3121 gtccggtcgt cgccggccaa tcgtcgggaa acgccggttc gacttgaaaa gggcatcaga
     3181 accagaaagc ggccaggcga cgaaatgttc atcgtcaggg cggctccctt cttgccgacc
     3241 tcttccttgg cgacctgaac caggacggtg tcgccgacgc cgaggctgac gtggccgttg
     3301 gcggcgaccg acttgccggt caactcaatc agcgaccgtt cgttgacttc gtccagcgga
     3361 agaaagcctt ccttgcccgt tccgtaatcg acgaaggctg cgtccagccc cttctcaatg
     3421 gtggtcacgc gaccacggta gatgtttccc ttcttcctgt tcgacccggc cgattcgatc
     3481 tccaggttaa taagcaggtc gccgtcaagc agcgccgcgc gggcctcata cgagtcagcc
     3541 gatataacga tttttcttgc caattcaaat tccattcaag gtcccggaat cgggacatca
     3601 gttgtcagat cccggcttcg cagggccggg actcctgttg accatgcgtc gaaccgcgag
     3661 catatccatg aacatcccgg ccgaatcgga aagggcgctg acccttgtcc gatcgtcgtt
     3721 gcgccaacga accggaacct cgtggatcgg gtatcccatc tgccttgcgc gggcaaggat
     3781 ctcgacgtca aacgcaaaac ggtcggtctg aagcaaaggc atcaggtttc gcaccacttc
     3841 gggcccgaag agcttgaagc cgcactgggt gtcgctgatg ccgccgatta cgaagattcg
     3901 aaccatcagg ttgaacacgc gccccatcag ttcccgataa agcggctgcc tgacttccag
     3961 gttcgaccct gccatgcccc ggctggcgat tacgacggtg tcgggcgccg cgtgaacggc
     4021 cagtttctca agttcctcga ttggggttga aagatcagcg tcgctcataa gaacgtacgg
     4081 aaaacgggcg tgggcgcatc cgttcttcgt cgagaatccc ttgccgcggt tgccggggtt
     4141 ggaaacgacg accagtcttt gcgaatcggg gatggttgac ctggcgacat cggcggtatt
     4201 atcggaagat ccgtcatcga cgacgattat ctcccagtcc gcaaggtttc gctcggcgta
     4261 agcgcggatt gccgacaggg tcgcaccgat ccttgatgac tcgttgtaac tggggataac
     4321 gattgaaatg ccgtctttcg gcgccggttg agtcatcact ggggtttggc ctcgaccttc
     4381 agaagctgtc gtccgacaag gattacatcg ccgtcacgga cggtgatgct gcccgaagtc
     4441 ttgatgtagg taccgttgcg gctttgcttg tccttcagcg caagtccgga cgaaaccggc
     4501 tcgaccgatg cgtgaacgcg ggacatgtag cgatcctccg gacatgagac gtccgcgact
     4561 tcacgcccga tgacgaccgc gcgccgtccc accggataga cctggccatc ggcgccgccg
     4621 gccagaatct ttgtcaccgt gccggccggg catgtctcgt cgggggacgc atagagccga
     4681 tagtcttcgg cgttaaggcc ggcggcttc
//
LOCUS       DAOPPA010000245         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19890_length_4690_cov_3.635167, whole genome shotgun sequence.
ACCESSION   DAOPPA010000245 DAOPPA010000000
VERSION     DAOPPA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19890_length_4690_cov_3.635167"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PKH54_08300"
     CDS             complement(<1..1665)
                     /locus_tag="PKH54_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99933.1"
                     /translation="MEDFSVAGVRLPSDLSELLAGRTRAAAVASHEHAAVIAALVGSA
                     ARRPVFLVAPDQAAVERISGLARYLVEFGPEPFDFPRWPSGLQSPYEQVVESPFVAAA
                     RLGALATCSLAETPFLLALDAARASARVIPFASFSDGLFQVQVGRPLNVEEVVGLLAR
                     AGYRRVSSVVEPGEFSIRNAVLDVFSPFHDEPFRAEVDYDEVETLRFFDPASQRTRRT
                     VDHVWIVPAWDVPCSEAAFRDAAISLRDAAAAGGVSTTVAGAIEFQLSQGRTPPGFPG
                     MLPFLYPEMELPVDYVGDDVAVIVVDPEGCRRSANEALDALAEARNAALEGSTALLEP
                     GERPGMMRPLAAEPDRLAVGGDALLQRLLSHPSAMILEVSPEGGILGPSHRLGSSVDL
                     VVCTAADVPVAERVAALRKIASDLVETGDSLLLVAPSEGEARRVSQILETGGLATEEA
                     SSFGLAALLAARAGIRVGSGRIRLPFGLESWGVFVMPSEAVFGVKDVLTRRQQERRNI
                     KKIQEFRELSRGDMVIHRDHGMGVFEGMMEVTVDGVTYKVFDVEADH"
     gene            complement(1703..3010)
                     /locus_tag="PKH54_08305"
     CDS             complement(1703..3010)
                     /locus_tag="PKH54_08305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC99934.1"
                     /translation="MTRGYVPASDAPCLIHMEDNRFPLVFFEVLVFGGSYDDPPGLEG
                     LADLTALNLLRGTTRRSHAEVMDGFNDLGASVDVTSHREYISISGDFMPRCMEPFTSL
                     LCEVLSRPAFPVQEFRKERALALEDIRNLVNDDAELARQHFHRYIYGDRLDGRPSSGW
                     FRTVSKISVQDCRDFYQGHFVSGNVAVVLAGAISRELADDFASRVVGSLRTGPSAVPP
                     EVDDSPVNGGRILIVDKPGRNQAQVVMGHPSAGWNCPDLFALLVGNTAFGGTFTSRLV
                     DEIREKRGWSYGVSSSIFAGRTSGTLMMRFFPENRDLAPAIELVEKLFSSTAFGGLSA
                     DEVDAARKYLIRQYPFRIETVRKRADELVADLVFGRPAGVMSRFVELVSSQDGQAVNQ
                     SLGRHFRPADLSTVLVGTAAEIRRDLVALAGAGSVDTVDYRSE"
     gene            complement(3007..4419)
                     /locus_tag="PKH54_08310"
     CDS             complement(3007..4419)
                     /locus_tag="PKH54_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC99935.1"
                     /translation="MHGLPSDLAGLQARAADISRRHPGMGSVRVLDVFEPSGGGQVVR
                     FQLDNGLQVILWPDHSAPLVSFQTWFKVGTVNECGFKNGIAHLFEHLMFKGTERYPHR
                     CFDKLLEEAGAQNNAATWLDWTFYYENLPSEALDLAFDLESDRMVNVVIDSKELDSER
                     DVVLSERRYRVDNDPDGEMEEALFASLFPNHPYGTPTLGTADAIESINLSDCRGFYRS
                     FYQPSNAILVIVGDCTYERAVELSLTRYGMIPGSPAIESPVPPFLPPRAGTSELALDV
                     NSERMRIGWVTVPAGDRVAVALDVANEILFANESSRVFKRLIDDLPLASDVDGTSEPM
                     RLAGTFIVDVIINEGEKAEDALSLVLEEIDRFGRDGPSAAELARACNHLEASFLRSMI
                     TVGSRATQLGIWHVTTGDYRQLFTFVDDVRAVDAESVRKAVADYLVPGLAVTILGRPS
                     TWPHGVVPLEAAGAETEVGR"
     gene            complement(4506..>4690)
                     /locus_tag="PKH54_08315"
     CDS             complement(4506..>4690)
                     /locus_tag="PKH54_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99936.1"
                     /translation="PGNDLLSRRPTPAEYLRAVNHAERLGFRHALVQEMEAADFYRPD
                     FNLKAEPFADADRFTK"
BASE COUNT         1010 a         1486 c         1385 g          809 t
ORIGIN      
        1 gtggtcggct tcgacgtcaa acaccttgta ggtgacgcca tcgacggtga cctccatcat
       61 cccttcgaac acacccattc cgtgatcacg gtgaatgacc atatccccgc gggaaagctc
      121 acggaactcc tgaattttct taatattgcg gcgttcctgc tgacgtcgtg tcaagacatc
      181 cttgacgccg aacacggcct cggaaggcat cacgaatacg ccccacgact ccaggccgaa
      241 tggaagcctg atccttccac tgccgaccct gatacccgcc ctggccgcaa gaagggcggc
      301 aaggccgaag cttgaagcct cctcggttgc caggccgccg gtctcaagaa tctgggagac
      361 gcggcgcgcc tcgccttcgg acggggcgac cagcagaagg gagtcgccgg tctcgaccag
      421 gtcggatgca atcttccgca gcgcggcaac acgctcggcc accgggacgt ccgcggccgt
      481 gcatacaaca agatcgaccg atgaaccaag cctgtgggaa ggccccagga tgccaccttc
      541 cggggaaacc tccaggatca tcgcggacgg atgcgaaaga agcctttgaa gcaatgcatc
      601 cccgccgacc gcaagccgat ccggttcggc tgccagcggg cgcatcattc cgggcctttc
      661 gcccggctcc agtaaggcgg tgctgccctc aagcgccgcg ttcctcgctt ccgccagcgc
      721 atcgagggcc tcgttcgcgc tgcgcctgca tccttcggga tcgacgacaa tgacggcgac
      781 gtcgtcgccg acataatcga ccggaagttc catctccgga tacaggaatg gcagcatgcc
      841 gggaaatccc ggcggggtgc ggccctggga aagctggaac tcgattgcgc cggccaccgt
      901 cgtcgatact ccgcctgcgg cggcggcgtc ccgaagtgaa atcgccgcat ccctgaacgc
      961 cgcctcggaa catggaacat cccatgccgg cacaatccag acgtgatcca ccgtccgacg
     1021 cgttctctgg gaagccgggt caaagaagcg aagggtctcg acttcgtcgt aatcaacctc
     1081 ggccctgaac ggctcgtcgt gaaatggcga aaagacgtca agcacggcat tcctgatcga
     1141 gaactccccc ggctccacca ccgaggacac tcgacggtag ccggccctgg caagcagccc
     1201 gacgacttcc tccacgttga ggggacgacc gacctgaacc tgaaagagcc catcggaaaa
     1261 ggaggcgaat ggaatgaccc tggcagacgc ccttgcggcg tcaagcgcaa gcaggaaggg
     1321 cgtctcggcc agggaacaag tggcgagcgc gccaagcctt gccgcggcga caaaaggact
     1381 ttcgaccacc tgctcatacg gagactgaag tccggatggc catcgcggaa aatcgaatgg
     1441 ctcgggaccg aactcgacaa ggtatcttgc aaggccggaa atccgttcca ccgcggcctg
     1501 atcgggggca acgagaaaga cggggcgccg cgcggccgag ccgacaagcg cggcgatgac
     1561 ggcggcatgt tcatggctgg cgaccgcggc ggcccgagtc cgccccgcca ggagttccga
     1621 caggtctgaa gggagcctta cgcctgcaac cgaaaaatct tccatctgca acggaatcgg
     1681 acccccgccg tcggccgggg cttcattcgc tcctgtaatc aacggtgtca acggaaccgg
     1741 caccggccag cgcgaccaga tcccgcctga tttccgccgc cgtcccgaca agaaccgtag
     1801 aaaggtcggc cggcctgaag tggcgcccga gcgactggtt cacggcttgc ccgtcctgac
     1861 tcgacaccag ttccacaaac cttgacatga cgcccgcagg acgaccgaaa accagatccg
     1921 cgaccagttc gtccgcacgc ttcctgaccg tctcgatccg gaaaggatac tgccttatca
     1981 ggtacttgcg cgccgcatcc acctcgtccg cggaaagacc gccgaaagct gttgaagaaa
     2041 acaacttttc taccagttcg atagccggtg ccagatccct gttttccggg aagaagcgca
     2101 tcatcagggt tccactggtt cttcccgcga agatgctgga cgaaacacca tagctccagc
     2161 ccctcttctc gcgaatctcg tcgaccagcc gggatgtgaa cgtccccccg aaggccgtgt
     2221 tgccgaccag aagggcaaaa agatcggggc agttccagcc ggccgagggg tgccccatca
     2281 cgacctgagc ctggttgcgg cctggtttgt cgactataag gatccttcca ccgttgaccg
     2341 gtgaatcgtc gacctccggc ggaaccgccg aagggccggt tcgaagggag ccgacaaccc
     2401 tggatgcgaa atcgtcggcg agttccctgg aaatcgcccc cgccaggaca acggcgacat
     2461 ttcccgaaac aaagtgcccc tgataaaaat cccgacagtc ctggacggat attttggaca
     2521 ccgtcctgaa ccatccgctg gaagggcgcc cgtcaagacg atccccatag atgtacctgt
     2581 gaaaatgctg ccttgccagt tcggcgtcgt cattgaccag attcctgatg tcctccagcg
     2641 ccagggcccg ttccttcctg aattcctgaa ccgggaaggc cggtcgggaa agaacctcgc
     2701 acagaagcga cgtgaagggt tccatgcacc ggggcatgaa atcacccgaa atcgagatgt
     2761 actcgcgatg cgaagtgaca tccacggagg cgccaaggtc gttgaaacca tccatgactt
     2821 ccgcgtggga tcgcctggtg gtgccccgga gcaggttcag ggctgtcaga tcggcaagcc
     2881 cttcaagtcc cggcggatcg tcgtatgaac cgccaaacac cagtacttca aaaaacacca
     2941 gcgggaagcg gttgtcttcc atgtgaatca ggcacggcgc atccgaggcc ggcacgtacc
     3001 ctctggtcat cggccgacct ccgtttccgc ccctgcggct tcaaggggaa cgacgccgtg
     3061 aggccaggtc gagggacgcc ccagtatcgt cacggcaaga ccaggcacca ggtaatcggc
     3121 gacggccttc ctgaccgact cggcatccac ggccctgaca tcatccacga aggtgaacag
     3181 ttgcctgtaa tccccggtgg tgacatgcca gatgccaagc tgggtcgccc gggagccgac
     3241 cgtgatcatc gatctcagga aggacgcctc gaggtgattg caggccctgg caagctcggc
     3301 ggccgatggg ccgtccctgc cgaacctgtc gatctcttca agaacaagcg acagggcatc
     3361 ctcggccttt tcgccctcgt tgatgatcac atccacgatg aaggttcccg caagccgcat
     3421 cggctcggaa gtgccgtcca cgtcggacgc aaggggaaga tcgtcaatca ggcgcttgaa
     3481 aacacgcgag gattcgttgg caaagagaat ctcgtttgcc acgtccagcg cgaccgccac
     3541 ccgatctccg gccggaacgg ttacccagcc aatcctcatg cgctcggagt tgacatccag
     3601 cgcgagttca ctggtgcctg cgcgaggtgg aagaaacggg ggaaccggcg actcgatggc
     3661 cggggagccg gggatcatgc cgtaccttgt caggctgagt tccaccgcgc gctcgtacgt
     3721 gcagtcgccg acgatcacga gaatcgcgtt cgacggctgg tagaagctgc gatagaaacc
     3781 acggcagtcg gacaggttga tggactctat tgcgtcggcg gtgccaaggg tcggcgtgcc
     3841 ataaggatga ttcgggaaaa gcgaagcaaa aagcgcttcc tccatttccc cgtccgggtc
     3901 attgtcgacc cggtatcttc tttcactcag cacgacgtcg cgctcggaat cgagttcctt
     3961 cgaatcaatt acgacgttga ccatccgatc ggattcaaga tcaaaagcaa gatcaagggc
     4021 ttccgatggc agattctcgt aatagaacgt ccagtcgagc caggttgcgg cgttgttctg
     4081 ggcccccgcc tcttcaagca gtttatcgaa acaccggtgg gggtatcgct cggttccctt
     4141 gaacatcagg tgttcgaaaa ggtgggcaat gccgttcttg aacccgcatt cgttgacggt
     4201 tccgaccttg aaccatgtct ggaacgacac caggggcgct gaatgatccg gccacaggat
     4261 cacctgcagt ccgttgtcga gctggaacct gacgacctga ccgccgccgg acggctcgaa
     4321 tacatcgaga acgcgcaccg agcccatgcc cggatgcctg cgggaaatat cggccgctcg
     4381 cgcctggagg ccggccagat ctgaaggaag tccgtgcatg aagctcccgg tccgtttccg
     4441 gcaacgtgcg aggatactac tttcatcggg aaagggggcg aataagaaaa cggccgacgg
     4501 gctgtttact tcgtgaaccg gtcggcgtcg gcgaacggtt cggccttgag gttgaaatcc
     4561 ggacgataga aatcggcggc ctccatctcc tggacaagcg cgtgcctgaa tccaaggcgc
     4621 tctgcatgat tcaccgcccg gagatactcg gccggggtcg gtcgccggga aagcagatcg
     4681 tttcccgggg
//
LOCUS       DAOPPA010000246         4669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_19996_length_4669_cov_3.977243, whole genome shotgun sequence.
ACCESSION   DAOPPA010000246 DAOPPA010000000
VERSION     DAOPPA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19996_length_4669_cov_3.977243"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PKH54_08320"
     CDS             complement(<1..1495)
                     /locus_tag="PKH54_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC99937.1"
                     /translation="MIWILIALPAICGVLVFLPLIGRLTRTILVGAAAAHLSLVLLIW
                     IARMVDVTPLLATTGLPTGALSIPMEAAGGWLGIDHLSLLVLTISSLLFMGASLYSLG
                     YLGPERKQRIKALTDELHVNNQPDAMFAACLLMFLSATTVVATARHLGILWVAVEATT
                     LATTPLIYFHRNRHSLEAAWKYLLLCSVGIAMALLGNFFLAVATAQGSGGELELLLPE
                     LQRHAGSMSPNWLRAAFLLMLVGYGTKMGLAPMHAWLPDAHSEAPSPVSALLSGALLN
                     CAFLAILRMLGVLDAAEMGDFGRKMLVGFGLLSMLWAAAMLIFQTDFKRMLAYSSVEH
                     MGILALGAGIGGIAATGSMFHALNHSLTKAALFLVAGNILARYKTKNIPDVRGLLSTM
                     RGDAVLWIAGILAITGAPPFGLFTSELMILRGIVSDGHWIIAVAFLLALAIAFASMLW
                     TLTRMTWGKVPEGIQLQDSSIRSMFSSSVPAVLLAGVLALGIWTADPV"
     gene            complement(1533..2177)
                     /locus_tag="PKH54_08325"
     CDS             complement(1533..2177)
                     /locus_tag="PKH54_08325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOC99938.1"
                     /translation="MMQALTELLAIVMILLNFRLLGGSRLKACIQALAIQALVLNILP
                     VLANFSRDPWVITLVIGSTLIKAVLLPALLMQAINKTGVTNEVKPIIGYTRSLLIGAA
                     MLALCALASRALKVPFSDAEQLLVPVSLFTILTGAFLLVARRKAINQVLGFLVLENGV
                     YLFGVTFAVSEPWLVQMGVLLDVLAAVFIMGIIIYHINREFGDIDIDRLSNLKD"
     gene            complement(2178..3089)
                     /locus_tag="PKH54_08330"
     CDS             complement(2178..3089)
                     /locus_tag="PKH54_08330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC99939.1"
                     /translation="MASAIVQFAAGLLLAPLLLTVIRRVKAFFGGRHGPTVMQPYIDL
                     AKLARKTPVYSRTTTWVFKASPVVGMAASIAALSILPLSGVPALAAFPGDFVFLAYVF
                     GMMRFFTVLAALDTGSPFEGMGASREVQFAVLSEVALFSCLIALVIALDATSLGSIFA
                     IAWGPAVQPGAAGILVLVACAMMLILLCENSRIPVDDPTTHLELTMIHEVMVLDHCGL
                     DLAMIEYASALKLWIWSAIVVSLALPVRTGSPWLDLLAGTIGIFILGAVIGVIESIMA
                     RLRLLKVPQLLVAACVLALLAVGLGLR"
     gene            complement(3093..>4669)
                     /locus_tag="PKH54_08335"
     CDS             complement(3093..>4669)
                     /locus_tag="PKH54_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC99940.1"
                     /translation="LMAWEGMSLASFFLVMYDHERAGVTRAGWIYMVTAHVGAAFLFV
                     MFLLLGHGGTLDFTSIIARSDVASDHVLASGVFILAVIGFGTKAGFIPLHIWLPEAHP
                     AAPSHVSALMSGVMIKTGIYGLLRVLTMIGAPQTWWGWVLVTVGAVSGVVGILFALAQ
                     HDLKRLLAYSSIENVGIITMALGLGLIGAASGQMLVASLAFSGALLHVVNHGFFKSLL
                     FMGAGSVIHGARTLQIDRMGGLLKNMPATGALFLVGSAAICGLPPLNGFAGEFLIYSA
                     AFFSIGAGTSIPFSSLTILALALIGGLAVATFTKAFGIAFLGEPRSAESSLASESGSA
                     MTIPMAMLAALCIAVALGAPWLANQIMPAVATFSPAMDNVLLPRAQSGIIMTITLISA
                     GFAVTIGLLAALRHALIRRRDADSAPTWDCGYAGQSSRVQYTGSSFAEPTARMFRPIL
                     RTRRNLTAPQGLFPTRAAISTHAADIFLQYVYEPAVAAISRAAALVQALQKGRVHLYL
                     MYIFITIVALMIWNLR"
BASE COUNT         1120 a         1365 c         1455 g          729 t
ORIGIN      
        1 acaccgggtc ggcggtccat atgccaagcg caaggacacc ggcaagtaag acggccggca
       61 cgctggagga aaacatcgac ctgatcgagg aatcctgaag ttgaatgcct tcaggaacct
      121 tgccccaggt cattcgggtc aaggtccaca gcatggatgc aaacgcgatc gccagcgcaa
      181 gcaggaatgc gaccgcgata atccagtgac cgtcggaaac gatgccgcgc aggatcatca
      241 gctcgcttgt gaaaagcccg aacggcggcg ccccggtaat cgccaggatg ccggcaatcc
      301 acagaacggc atcgccgcgc atcgttgaca gcaggccgcg cacatcagga atgttcttgg
      361 tcttgtaacg cgccaggatg ttcccggcga ccaggaaaag cgcggccttt gtcagggaat
      421 ggttcagtgc gtgaaacatt gaaccggttg ccgcgatgcc gccgatgccc gcacccaggg
      481 caagaattcc catgtgctcg acgcttgagt atgcgagcat gcgtttgaag tctgtctgga
      541 atatcagcat cgccgcggcc caaagcatcg aaagaagtcc gaatccgacc agcatttttc
      601 gcccgaaatc acccatttcc gcggcgtcca gcactccaag cattcgcagg atggcaagga
      661 atgcgcagtt cagaagcgcg ccggaaagaa gggcggaaac cggcgaaggc gcttcgctgt
      721 gggcatccgg aagccaggcg tgcatcgggg ccaggcccat cttggttccg tatcccacga
      781 gcatcaggag gaaagcggcc cgcagccagt tcggcgacat cgaaccggcg tggcgttgca
      841 gttcgggcag cagcagttcg agttcacctc cgctgccctg ggcggtggcc acggcaagga
      901 agaagtttcc aagcagcgcc atcgcgatcc ccacgctgca aagcagcagg tatttccagg
      961 cggcctccag gctgtgccgg ttgcggtgga agtatatcag cggagtcgtt gcaagagtcg
     1021 ttgcctcaac ggccacccac agtatgccaa ggtggcgcgc ggtcgcgaca accgtcgtcg
     1081 ccgacaggaa catcaggaga caggccgcga acatggcgtc cggctggttg ttgacgtgca
     1141 gttcgtcggt cagtgccttg atccgctgtt tgcgttccgg gccgagataa ccaagcgaat
     1201 atagtgatgc gcccatgaaa agcaggcttg aaatcgtcag gacaagcagc gacaggtgat
     1261 cgatgccaag ccagcccccc gcggcctcca ttggaatcga caacgcgccc gtcggaagtc
     1321 cggtcgtcgc aagcagcggc gtcacatcga ccatccgggc aatccagatc agaagcacaa
     1381 gcgaaaggtg agcggcggcg gccccaacca ggatggtccg cgtcaggcgc ccgatcaggg
     1441 gcaggaacac aagcacgccg caaatcgccg gaagtgcaat cagtatccag atcatcgaaa
     1501 gcgtcccgcc gggcacgatt gcccgatcaa actcaatcct tcaggttcga caggcgatcg
     1561 atgtcgatgt cgccgaactc gcgattaatg tgataaatga tgattcccat gatgaaaacg
     1621 gccgccagca cgtccaggag cacgcccatc tgcaccagcc atggctcgct gaccgcgaaa
     1681 gtcacgccga acagatagac gccgttttca agcacaagga atcccagaac ctggttgatg
     1741 gccttgcggc gggcaaccag aaggaaagcg ccggtcagga tggtaaacag cgataccgga
     1801 acaagaagct gttcggcgtc ggaaaaaggc accttcaagg ctcgggatgc cagcgcgcac
     1861 agagccagca tcgcggcccc gatcagcagc gaccttgtgt aaccgataat cggcttgacc
     1921 tcgttcgtga cgccggtctt gttgatggcc tgcatcagca gtgccggaag aagtacggcc
     1981 ttgatgaggg tcgaaccgat gaccagggtg ataacccatg gatcccgcga gaagttggcc
     2041 agaaccggaa gaatgttaag gacaagcgcc tggattgcca gggcctggat acacgccttc
     2101 aggcggcttc cgccaagcag ccggaagttc agaaggatca tgacgattgc cagcagttcg
     2161 gtaagcgcct gcatcattca ccttaagcca aggccgacgg ccagcagtgc aagcacacag
     2221 gcggcgacca gcaattgggg aaccttcaga agccgaagtc ttgccatgat ggattcgatg
     2281 acgccgatta cggcaccgag tatgaaaatc ccgatcgtgc cggcaagaag gtccagccac
     2341 ggtgagccgg tccggacggg cagggcgagc gagacgacga ttgccgacca gatccacagc
     2401 ttcagtgccg aagcgtattc gatcatggca agatccagtc cgcagtgatc cagaaccatt
     2461 acttcgtgga tcatcgtcaa ctcgaggtgt gtggtcggat catcgaccgg tatccgcgag
     2521 ttttcgcaaa gaagaatcag catcatcgcg cacgccacca ggacaaggat tccggccgcg
     2581 ccaggctgga cggcggggcc ccaggcaatc gcgaaaatcg agccaagcga agtggcgtca
     2641 agcgcgatga ccagtgcgat gaggcaggaa aacagcgcaa cttcagacag aaccgcgaac
     2701 tgcacttcgc ggctggcacc catgccttcg aagggcgagc cggtgtccag cgcggcaaga
     2761 accgtgaaga accgcatcat tccaaagacg tatgccagga atacgaaatc gccggggaag
     2821 gcggccagcg ccgggacgcc ggacagggga agaatggaaa gcgccgcgat cgaagcggcc
     2881 atcccgacaa cgggacttgc cttgaagacc cacgtggtcg ttcggctgta aaccggggtc
     2941 ttgcgcgcca gcttcgcaag gtcgatgtac ggctgcatga ctgttggtcc gtggcggccg
     3001 ccgaaaaatg ccttgacccg gcggatgacg gtcagcagca acggggcaag aagcaggccg
     3061 gcggcaaact gtacgattgc ggatgccatg gcctacctca ggttccagat catcagggcg
     3121 acgatcgtga tgaagatgta catcagataa agatggacgc gtcccttctg cagggcctga
     3181 acaagcgcgg cggcccgcga aatcgccgcg acggccggtt catagacgta ctgcaggaaa
     3241 atatccgcgg catgcgtcga aatggccgcc ctggtcggga aaagcccctg gggcgccgtc
     3301 aagttgcgtc tggttcgaag gattggccgg aacatccgtg cggtcggttc ggcaaaggat
     3361 gaaccggtgt actgcacccg cgatgactgg ccggcgtacc cgcaatccca tgtcggggcg
     3421 gaatcggcgt cgcgtcgtcg aatcagcgcg tggcgaagcg cggccaacag gccaatcgtg
     3481 accgcgaaac cggccgaaat caacgttatc gtcatgatga tgccggactg ggcccgcgga
     3541 agcaggacgt tgtccattgc gggggaaaac gtcgcgaccg ccggcattat ctgattcgcc
     3601 agccacggtg cgccaagagc gaccgcaatg caaagggctg caagcatcgc catcggaatc
     3661 gtcatggcac tcccggattc ggaggccagt gatgactcgg ccgatctcgg ttcgcccagg
     3721 aaggcgatcc cgaaagcctt cgtgaaggtt gcgacggcca ggccgccgat cagggcaagc
     3781 gcgagaatgg taagtgacga gaagggaatc gaggtgcctg cgccgatgct gaagaaggcc
     3841 gccgagtaga tcagaaattc tccggcgaac ccgttcagtg ggggaaggcc gcagatggct
     3901 gccgacccga caaggaaaag cgcgccggtg gccggcatgt ttttcaggag tccccccatg
     3961 cggtcaatct gcagggttcg ggcaccatga atcaccgaac cggcacccat gaacagaagc
     4021 gatttgaaga atccgtggtt gacgacatga agcagggcgc ccgaaaaggc cagcgacgcc
     4081 accagcatct ggccgcttgc ggcgccaatc agcccaaggc ccagggccat cgtgataatg
     4141 ccgacgtttt cgatacttga ataggccaga aggcgcttca gatcgtgctg ggccagtgcg
     4201 aagaggatgc cgaccacgcc cgacacggca cccacggtca caagcaccca gccccaccag
     4261 gtctggggag cgccgatcat tgtaagaaca cgaagaaggc cgtaaatgcc ggtcttgatc
     4321 atcacgccgc tcatcagcgc cgaaacgtgg gatggagcgg ccggatgcgc ctctggaagc
     4381 cagatgtgaa gcgggatgaa cccggctttc gttccgaagc cgatcaccgc gaggatgaac
     4441 accccggaag ccaggacgtg atccgaggcg acatcggacc tggcaataat cgatgtgaaa
     4501 tcaagcgttc cgccgtggcc aagaagaagg aacatcacga aaaggaacgc cgcgccaacg
     4561 tgtgcggtga ccatgtagat ccagcccgcg cgagtgacgc ccgccctttc gtggtcgtac
     4621 atgaccagga agaacgatgc cagcgacatg ccttcccagg ccatcagga
//
LOCUS       DAOPPA010000247         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20212_length_4627_cov_5.710192, whole genome shotgun sequence.
ACCESSION   DAOPPA010000247 DAOPPA010000000
VERSION     DAOPPA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20212_length_4627_cov_5.710192"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKH54_08340"
     CDS             complement(<1..690)
                     /locus_tag="PKH54_08340"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOC99941.1"
                     /translation="MVHGFWRSPLKHQQAVIDIPRQVPWNLSIQNGVMMKKSEFDESV
                     AGVAPVASGLPPDDALFSRVSAILDQAHKNVVRAVNHNMVTAYWLIGREIVIELQRGE
                     ARAGYGEEVLKELAARLTPLFGAGYSQTNLRNFRFFYLAYCDADGQIQHTVCAESGDG
                     HKKFNEDRGLVPVFSSQLSWSHYRALMRIQNRAARTFYEIESDKGGWSVRELERQIHT
                     QYYERLVATQIQ"
     gene            complement(660..1331)
                     /locus_tag="PKH54_08345"
     CDS             complement(660..1331)
                     /locus_tag="PKH54_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99942.1"
                     /translation="MSIQNEPPVPNVKPFATDLEYLMAEFEWVQARTKRIEAGQLAMN
                     QAPALAKMQSGRMRAVYADATAGMAGQLAREEQDIRQEIDARMAVNRAVGPKLGLDRL
                     CREHGLGQFERAALLLATIPCIGYSMMETHVTPLRPRYSSTTIDAGIVSLFLELDMKG
                     CLDGLLCLLPDAPLRLDGLVALTYEPRTPASALDAGFELTGKTLAAITGISAFEWFTD
                     SGAAH"
     gene            complement(1480..>1840)
                     /locus_tag="PKH54_08350"
     CDS             complement(1480..>1840)
                     /locus_tag="PKH54_08350"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOC99943.1"
                     /translation="MSPRHIAEVCAEIRPVLARCGDAVLLVHDAVIQEVVRGNQAILA
                     WLEAGWTRGGGGTDHCPVFDWIARAAIDPDLYVGLTDCASLYPEVPPAYPVVWVTPRA
                     SNYSIAPWGTRLWADPF"
     assembly_gap    1841..1940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1941..2515)
                     /locus_tag="PKH54_08355"
     CDS             complement(<1941..2515)
                     /locus_tag="PKH54_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99944.1"
                     /translation="MSPPARKQPSRDEIIAREKRRTAAIRMQLTVNHPFWGYLLIETE
                     LIPAVDLPSLAATDCERRIWYNPTLTQYLTLDELGFVMAHEVGHIVLQNLERCRGRKA
                     SVWNAACDYAVNRIVAAITDPYDGLPLYTPPVKFLPTVGRIAPLMNPDFDELTTEAIY
                     EELRENPMWDDSEHLVEVDLSSGETGTVRLDR"
     gene            complement(2515..3711)
                     /locus_tag="PKH54_08360"
     CDS             complement(2515..3711)
                     /locus_tag="PKH54_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC99945.1"
                     /translation="MSVDRSSAVNLDDDDGADFAEVTDDCAEVVDEAPEAPLPDLTGD
                     PVRHDGYSYDELCPMVEALLRGGLSVLVRGHPGVGKSSLAKELAARMELPMVDIRLAQ
                     RDPAEISGVYYPDADRTCMSLLAPDWVRQVCESPGFVFLDEINAAVTKLHQAAAYQIV
                     LERRVGPFVFHPGTVVLAAGNLEEDNAIVSPMSSALANRFAHFTMRVDADSWMRWASR
                     AGIDDAVTAYIGRGGEGVLYDRRRDAMVFPSPRTWEMASRALAGARPVDRRRVVAACV
                     GIDAAERFSSFLTIFERVDPRAVILDGESMHFKTPRNKDPSFVHAAVLTVADWLVRRP
                     DVPEAALPNVVRFLRSSGLDPEFAFLFLRRLKGSGLLTRLRAVPEYRVLAADLASIHI
                     GLTGGR"
     gene            complement(3684..4058)
                     /locus_tag="PKH54_08365"
     CDS             complement(3684..4058)
                     /locus_tag="PKH54_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99946.1"
                     /translation="MARRDMEAVDSQLLERARGTLRHSRFVRHDGAGDSGAAGVGDAA
                     GGGAAAGAGDKTGRLVSFSVIERTDKNTVTADLDWGQAPTCTCTSYWTEVRLGRGPWC
                     RHVVAVLVKEEELRCQLIDLLL"
     gene            4206..>4627
                     /locus_tag="PKH54_08370"
     CDS             4206..>4627
                     /locus_tag="PKH54_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99947.1"
                     /translation="MTTDELAMLLTQTMKKDLADDRKSWGPFGKYVFNAKSGLVWMSS
                     PSGGNVAYFEPEADGAFLVTAIVSVAIECNGATDEELQEEALDSLENELWPALHAAGY
                     STEPGEDSWAPEISSLSRFGVKKVNSVAELIAEARFLS"
BASE COUNT          892 a         1448 c         1324 g          863 t
ORIGIN      
        1 ctgaatctgg gtggcgacca gccgttcata gtactgtgtg tgaatctggc gttcgagttc
       61 ccggacactc cagccacctt tatcagattc aatttcgtaa aaagtccgcg ctgcccgatt
      121 ctggatccgc atcaatgccc ggtagtgaga ccatgaaagt tgcgatgaaa aaacgggaac
      181 caacccccgg tcctcgttga actttttgtg tccgtcaccg gattcagcac acaccgtgtg
      241 ctgaatctga ccatcggcat cacagtaagc cagatagaag aacctgaaat tcctgagatt
      301 ggtctgggaa taccccgcgc caaaaagcgg cgtgaggcgt gccgccagtt ctttcagaac
      361 ttcttccccg tatccagccc ttgcctcgcc gcgctgaagt tcgatcacaa tctcgcggcc
      421 gatcaaccag tacgcagtga ccatgttgtg gttaacagcc cgcacgacgt ttttgtgtgc
      481 ctgatccaga atcgctgaaa cgcgactgaa caacgcatcg tccgggggaa gaccggaagc
      541 cacgggagcg acgcctgcaa ccgattcatc aaattcagac tttttcatca tgaccccgtt
      601 ttggatggat aaattccatg gcacttggcg gggtatatcg attactgctt gctggtgttt
      661 cagtgggctg cgccagaatc cgtgaaccat tcaaaagccg aaatgccggt gatggcggcc
      721 agggtcttgc cggtcagttc aaacccggca tcaagggccg aggccggcgt cctgggctcg
      781 taggtcagcg cgaccagccc atccaggcgc aggggcgcat cgggcaacag gcacaacagg
      841 ccatcaagac agcccttcat gtccagttcc aggaacagcg acacgatgcc ggcgtcgatg
      901 gtcgttgaac tgtagcgtgg ccgcaggggc gtcacgtgcg tttccatcat cgagtatccg
      961 atgcagggaa tggtcgccag caacagggcg gccctttcaa actgccccag cccgtgttcg
     1021 cggcagaggc ggtccagacc aagtttcggc ccaaccgcgc gatttaccgc catccgcgcg
     1081 tcgatttcct gcctgatgtc ctgctcttcg cgggcaagct ggccggccat gccggccgtg
     1141 gcgtcggcat agaccgcacg catcctgccc gactgcatct tcgccagcgc cggggcctgg
     1201 ttcatcgcca gttgaccagc ctcgatccgt ttggtgcggg cctgcaccca ctcgaattcc
     1261 gccatcaggt attccaggtc ggtcgcaaag ggtttcacgt tcgggaccgg tggttcgttc
     1321 tggatgctca tttttacctc ctgtggtctt gagttcatcc agtaccacta ctgaggagtt
     1381 cggagatcta agccggaaat ctttggaatc ctggaaaata gttcaagcaa acgcttttag
     1441 ccgtgcagat ttcgctgtaa ccaaacgtac ggcgggccat caaaacggat cggcccacag
     1501 gcgggtgccc cagggggcaa tgctgtagtt agaggcccgg ggcgtgaccc agacgaccgg
     1561 gtacgcgggc gggacctcgg gatacagtga tgcgcagtct gtcaggccaa cgtacaagtc
     1621 ggggtcgatg gcggccctgg caatccagtc aaagacaggg cagtggtcag tgccgccccc
     1681 gccacgggtc cagccggctt caagccacgc aaggatggcc tgattgcccc tgacgacctc
     1741 ctggatgacg gcgtcgtgga ccagcaggac cgcatcgccg caacgcgcca gcaccgggcg
     1801 tatctcggcg cagacttcgg cgatatgtcg tggcgacatg nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn cgatccagac gaacggtccc cgtttcccca gatgacaggt
     1981 ccacttcgac cagatgctcg gaatcgtccc acatcgggtt ttcgcgcagt tcttcataga
     2041 tggcttccgt ggtcagttca tcgaagtccg gattcatcag gggcgcgatt cggccaaccg
     2101 tcggcaaaaa cttcaccggg ggcgtgtaaa gcggcaggcc gtcgtacggg tcggtgattg
     2161 ccgcaacaat gcggttcacg gcgtagtcgc acgcggcgtt ccacaccgac gccttgcggc
     2221 cgcggcatcg ctccaggttc tgaagcacga tgtggccgac ctcgtgggcc atcacgaatc
     2281 ccagttcgtc aagggtcagg tactgggtca acgtcgggtt gtaccagatg cggcgttcgc
     2341 agtccgtggc ggccagcgac ggcagatcga ccgccgggat aagctcggtc tcgatcagca
     2401 ggtatcccca gaacgggtga ttgacggtca actgcatcct gatggccgca gtccgccgct
     2461 tctccctggc gatgatttcg tcccgcgaag gttgttttct ggctggcggc gacatcacct
     2521 gccccccgtc agcccgatgt ggatggatgc caggtcggcc gccaggacgc ggtattccgg
     2581 cacggcccgc aggcgggtca gcagtccgga gcccttcagg cgccgcagga acaggaaggc
     2641 gaattccggg tccaggccgg acgatcgcag gaaccgcacg acgttcggca gggcggcttc
     2701 gggcacgtca ggcctgcgca cgagccagtc cgccaccgtc aggaccgccg cgtggacaaa
     2761 cgacggatcc ttgtttcgtg gcgtcttgaa atgcatcgac tcgccatcca ggatcactgc
     2821 cctgggatcg acccgttcaa agatcgtcag gaacgaactg aaccgctcgg ccgcgtcgat
     2881 tccgacgcac gcggcaacga ccctgcggcg atcgaccgga cgcgccccgg ccagggcccg
     2941 gctggccatc tcccacgtcc tgggcgacgg aaagaccatg gcatccctgc gacggtcgta
     3001 aagcacgcct tcgccgcccc tgccgatgta cgcagtgacc gcgtcgtcaa tcccggcccg
     3061 gctggcccag cgcatccacg aatcggcgtc gacgcgcatc gtgaagtgcg cgaaccggtt
     3121 ggccagcgcc gacgacatgg gcgacacgat ggcgttgtct tcttccaggt taccggcggc
     3181 cagcacgaca gtccccgggt ggaacacaaa cgggccgacg cggcgctcga gcacgatctg
     3241 gtacgccgcc gcctggtgca gtttggtcac agccgcattg atttcatcca gaaacacgaa
     3301 ccccgggctt tcgcagacct gccgcaccca gtcgggggcc agcagcgaca tgcacgtgcg
     3361 atcggcgtcc gggtaataca cgccagaaat ctcagccggg tcccgctgtg ccaggcggat
     3421 atcgaccatc ggcagttcca tccgcgccgc cagttccttc gccagcgacg acttgccgac
     3481 accgggatgc ccgcgcacca gcaccgacag cccgccccgc agaagggctt caaccatcgg
     3541 acaaagctcg tcgtacgaat agccgtcgtg cctgaccggg tcgccagtta gatcggggag
     3601 tggtgcttcg ggggcttcgt ccacgacttc ggcacagtcg tccgtgactt cggcgaaatc
     3661 ggccccgtcg tcgtcatcaa ggttcacagc agaagatcta tcaactgaca tcgcagttcc
     3721 tcctctttca caagcaccgc gaccacatgc cggcaccaag gcccccgccc cagccggact
     3781 tccgtccagt aactggtgca tgtgcacgtc ggggcctgcc cccagtcgag gtcagccgtc
     3841 accgtgtttt tgtcggttct ttcgattacc gaaaaactga ccaggcgacc ggttttgtcc
     3901 ccggcaccgg cagcggcccc gccaccggcc gcgtcgccaa cccccgccgc gccactgtcg
     3961 ccagccccgt catgcctgac gaaccgcgag tgccgcaggg ttccccttgc cctttcaagc
     4021 agttgtgaat caaccgcctc catgtccctt cttgccattc ctgcctccgt ggatgttggc
     4081 aaccaaggta cctactgggg agttggaaaa tctaagcgga attttttgtc ggaccggcca
     4141 ggtattgtcc gttctgcccg gggtgataga ctctgcatca gtttcaaacg aacgaggccc
     4201 ccgaaatgac caccgacgaa cttgccatgc ttctgaccca gacgatgaag aaagatcttg
     4261 ccgatgaccg aaagtcctgg ggtcccttcg gcaagtacgt tttcaatgcc aaatcaggtc
     4321 ttgtctggat gtcctctcca agcggcggga atgtcgcgta ttttgaacct gaagccgacg
     4381 gggcattcct tgtcacggcc atcgtttcgg tggcaatcga atgcaacggc gcaaccgatg
     4441 aagaactgca ggaggaggcc ttggacagtc tcgaaaacga gctgtggccc gccctgcacg
     4501 cagcaggtta ttccaccgaa cccggggaag acagctgggc ccctgaaatc agttcccttt
     4561 cccgcttcgg cgtcaagaag gtaaactccg tcgccgaact catcgcagaa gcccgcttcc
     4621 tgtccag
//
LOCUS       DAOPPA010000248         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20226_length_4626_cov_4.064756, whole genome shotgun sequence.
ACCESSION   DAOPPA010000248 DAOPPA010000000
VERSION     DAOPPA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20226_length_4626_cov_4.064756"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKH54_08375"
     CDS             complement(<1..287)
                     /locus_tag="PKH54_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC99948.1"
                     /translation="MLYMLDTNICAYILKNRPITVKAHFDDVPPESLAISAIVMAELA
                     FGVELQPGNERLRRQVADFTSRLRVIPWDDGAARHYGNIRADLHRAGAMVG"
     gene            complement(287..517)
                     /locus_tag="PKH54_08380"
     CDS             complement(287..517)
                     /locus_tag="PKH54_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOC99949.1"
                     /translation="MTRTAKLFMNGRSQAIRLPAEFRFKSREVLVEKQGDTLIVRPKP
                     DGWDDFFASDERTPEDFLACRDNTPPQERQVF"
     gene            726..2033
                     /gene="rmuC"
                     /locus_tag="PKH54_08385"
     CDS             726..2033
                     /gene="rmuC"
                     /locus_tag="PKH54_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOC99950.1"
                     /translation="MTNILLIIVALLVTVAVAMLAAVLSALRKPPAVDLDRTLREEMR
                     LARDENANAARALREEITATQANANQTLVTTLDNMGERQRRRLDDVAKSYADGQDRMR
                     TLLETRLDVLQRGNEAKLDEMRRTVDEKLQSTLEKRLGESFKIVSERLEAVQQGLGEM
                     RNLASDVGGLKRVLTNVKDRGTWGEYQLGAILAEILTPDQYARDFSPRDNRDHVEFAV
                     RLPGQGEDSEHPVWLPIDSKFPKEDYERLLDAVERADAEQIKQSGKALGNAVRLAAKT
                     IQEKYVEPPRTTDFGIMFLPSEGLYAEVLRQPGLVTEIQNTYRVTVAGPTTISALLTS
                     LRIGFQTVAIEKRSHEVWKVLAAFKTEFGKFGEVLKNVQKKLNEASSALDKSAVRTRA
                     MQKVLKNVEQLPAGEVDALLGAPAVDVADEEPDEDGPSDVGLT"
     gene            complement(2109..3854)
                     /locus_tag="PKH54_08390"
     CDS             complement(2109..3854)
                     /locus_tag="PKH54_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOC99951.1"
                     /translation="MTDITGVWPKDGPRPHESDGERKVYDALKAGMPAGWTAWHSLKV
                     RGPDGAEAEGDFVIAAPGHGMVVIEIKDGRISVRDGQWYQNGRLMKRRPRDQALRFAK
                     ILDSRLNDCRCRAPFAVMTIFPDTPFDNAPSQDDLRGAVFGEQSLPQLCEIIFRGIER
                     IIPATFGPPSGPWIEELHRLWGETWLPRLSLGNRHELNEERRIRMDNTQRCLLDQTEK
                     TRLALVYGMAGSGKTMLAREKAIHAMREGRKVQVLCFTDPLAAGLSAALNEHGVPVDT
                     VRQYAASLLKKAGLPESDDKTSDFWDQVSLRAARDAIGAVERFPDLLIVDEAQDFSKG
                     DWELVRVLSQKDDGVCNCWAFCDMTQAYWEDRGLPEWLKRGMVFDLENNYRCPPPIMA
                     LARLVAGEIPAMDGSVPLPVASTGTATEIDSIPEQTLDLIREGLESGLIRITETRNTA
                     QIQAAVDAEVAALLKGGLEPGDIAVISLCGREVKDSITKSKMFAGKAVLPADNPKAGQ
                     RLVADTFLRFKGLERSAVIVTDMYQLAAQRNLAVRLFIAMTRAQDFLRLVVAPGDLSR
                     LPALRVAAALRNTAN"
     gene            4066..4392
                     /locus_tag="PKH54_08395"
     CDS             4066..4392
                     /locus_tag="PKH54_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99952.1"
                     /translation="MDELTLDGITGLGSSIQAILSPIWLDEQWQLLKDLEDVRDNAAA
                     IAEMKEIMSGPLWPVEEKPKAGRRRRSFMASELRKPDDNGVFRLIGVMVVSKTKGTFR
                     PNLGYG"
BASE COUNT          922 a         1385 c         1408 g          911 t
ORIGIN      
        1 tttccgacca tggcgccggc ccggtgcaga tcggccctta tgttgccgta gtgcctggcc
       61 gcgccgtcat cccatggaat gacccgcaac ctggaagtga aatccgcaac ctggcggcgc
      121 agccgttcgt tgcccggttg cagttcgacc ccgaaagcca gttccgccat gacaatcgcg
      181 gaaattgcca ggctttcggg agggacgtcg tcgaagtgcg ccttcaccgt gatcggccgg
      241 tttttcagga tgtacgcgca gatgttggtg tcgagcatgt acagcatcag aagacctgcc
      301 tttcctgggg cggcgtgttg tcgcggcagg ccaggaagtc ttcaggagtc ctttcgtcgc
      361 tggcgaagaa atcgtcccac ccgtccggct tggggcggac gatcaatgtg tcgccctgtt
      421 tttcaaccag cacttcgcgg gacttgaagc ggaattccgc tggcaggcgg atggcctggc
      481 tgcgtccgtt catgaacagt ttggcggttc gcgtcatttc agcgtcttcc gatatagacc
      541 acatgatata tatcctttcc ctgtgggaat ttcaaggcgg ccggggtttt gtcgccgagt
      601 ctgtcggttt gttccggcaa agacggcggg aagaccggaa cttcgcaact ttcctgattt
      661 gcggcagaaa tcttgccgcg ggctcgggta tgcttggccg tattgaacga accgggtgat
      721 gacgaatgac gaacatcctg ctgattatcg ttgcgctgct ggtcaccgtg gccgtggcga
      781 tgctggctgc ggtgctgtcc gcgctgcgca aaccgccggc ggtcgatctg gaccgcacgc
      841 tgcgtgaaga aatgcgcctg gcgcgtgatg aaaacgcgaa cgccgcccgg gccctgcgcg
      901 aagaaatcac ggccacgcag gcgaacgcca accagacgct ggtgaccacg ctggacaaca
      961 tgggtgaacg gcagcgccgc aggctggacg acgtggcgaa gtcatacgcc gacggccagg
     1021 accggatgcg cacgttgctg gaaaccaggc tggacgtgct gcagcgtggc aacgaagcca
     1081 agctggacga aatgcgccgg acggtggatg aaaaactgca gtccaccctg gaaaagcgcc
     1141 tcggcgaatc tttcaagatc gtcagcgaac ggcttgaagc ggtccagcag gggcttggcg
     1201 aaatgcgcaa cctggcgtcc gacgtgggcg gcctgaaacg tgtcctgaca aacgtcaagg
     1261 accgcggcac ctggggcgaa taccagctcg gcgccatcct ggccgaaatc ctgacgccgg
     1321 accagtatgc ccgcgacttt tcgccccgcg acaacaggga tcacgtggaa ttcgcggtgc
     1381 ggctgccggg tcagggcgag gactctgaac atccggtctg gttgccgatc gactcgaagt
     1441 tcccgaagga agattatgaa cgcctgcttg acgctgttga acgcgccgac gccgaacaga
     1501 tcaagcagtc gggcaaggcg cttggcaacg cggttcgtct tgcagcgaag accatacagg
     1561 aaaaatacgt cgaaccaccc agaaccaccg atttcggcat catgttcctg ccgtcggaag
     1621 gtctgtacgc tgaagttttg cgccagccgg gcctggtgac tgaaatccag aacacgtacc
     1681 gcgtgaccgt ggctggcccg acgaccattt ctgccctgct gaccagcctc cggatagggt
     1741 tccagaccgt tgcgatcgaa aagcgatcac acgaggtttg gaaggtgctg gccgccttca
     1801 agacagaatt cggcaagttc ggcgaggtcc tgaaaaacgt ccagaagaag ctgaatgagg
     1861 cttccagcgc cctggacaag tccgccgtac gcacacgtgc catgcagaag gttctgaaaa
     1921 acgtcgagca acttccggcc ggtgaagtcg atgccttgct tggcgccccg gccgtggacg
     1981 tcgccgacga agaaccggat gaagacggtc cgtccgacgt ggggctgacg tgacacactg
     2041 gaataggtgt cgcctgtccg tcgcctaacg cggccccctg tcaggacatg ctgattgaac
     2101 gagtcttttc aattggccgt atttctcagc gccgcggcca cccgaagggc cggcaggcgg
     2161 ctcaggtcgc cgggcgccac gaccaggcgc aggaagtcct gggcacgggt catcgcaatg
     2221 aagagccgca cggcaagatt gcgttgggcc gccaattgat acatgtctgt gacgatgaca
     2281 gccgatcttt ccagtccctt gaaacgcagg aatgtatcag cgacgagccg ctggcctgcc
     2341 ttcggattgt cggccggaag aaccgccttg ccggcgaaca tctttgattt cgtaatgctg
     2401 tccttcactt cgcgtccaca cagtgaaatg acggcgatat cgcccggttc aagcccgccc
     2461 ttcaacagcg cagccacctc ggcgtccact gccgcctgga tctgcgccgt gtttctggtt
     2521 tcggtgatgc gtatcagccc gctttcaagt ccttccctga tcaggtcgag ggtttgttcg
     2581 gggatactgt cgatttccgt cgcggtcccg gtcgatgcca ccggcagtgg cacggaaccg
     2641 tccatggccg ggatttcgcc tgcgacaagg cgcgccaggg ccatgatcgg cggcgggcac
     2701 ctgtaattgt tttcgaggtc aaagaccatc ccgcgtttca gccactcagg cagcccgcga
     2761 tcttcccagt acgcctgcgt catgtcgcag aaggcccagc agttgcaaac cccgtcatcc
     2821 ttctgtgaca ggacgcggac cagttcccag tcgcctttcg agaaatcctg ggcttcatcg
     2881 acgataagca gatccgggaa ccgttcgacc gcaccaatcg cgtccctggc tgccctgagc
     2941 gatacctggt cccagaagtc agaggttttg tcatcagatt ccggcagccc ggccttcttc
     3001 agcagggacg ccgcgtattg cctgacagta tcgacaggca caccgtgttc attgagcgcc
     3061 gccgaaagac ccgcggccag cggatcggta aaacagagta cctggacttt gcggccttcc
     3121 cgcatcgcgt gaatcgcctt ttcccgcgcc agcatcgtct tgcctgatcc agccatgcca
     3181 taaaccaggg ccagcctggt tttttcagtc tggtccagca ggcatcgctg ggtgttgtcc
     3241 atcctgatgc ggcgttcttc gttcagttca tggcggttac ccaggctcag ccttggcagc
     3301 cacgtttcgc cccagagcct gtgcagctct tcgatccagg gcccggaagg cgggccgaag
     3361 gtggcgggaa tgattctttc gattccgcgg aaaatgattt cgcaaagctg gggcagggac
     3421 tgttccccaa aaacggctcc ccgcaggtcg tcctgggaag gagcgttgtc aaaaggcgtg
     3481 tccgggaaga tggtcatcac agcaaaagga gccctgcacc gacagtcgtt cagccttgaa
     3541 tcgagtatct tcgcgaaccg gagcgcctga tccctggggc gccgtttcat caggcgcccg
     3601 ttctggtacc actggccgtc ccgcaccgat atccggccat ccttgatttc gatcacgacc
     3661 atcccgtggc ccggggccgc gatgacgaaa tcgccctcgg cctcggcccc gtcagggcca
     3721 cggactttca ggctgtgcca ggcggtccac ccggccggca taccggcctt gagagcgtcg
     3781 tacaccttgc gctcgccatc cgattcgtgc ggccgcggtc catccttggg ccagacgccg
     3841 gtgatgtctg tcattaccaa ttaccccgca ctggccttgt accccaataa tggacatgtc
     3901 agtaatgctg ccacggcagc gccagaaagg acaagccttt cgtaatcggc cggactcagg
     3961 tacccgtttg cagcgatggc ggcgcacaac ggatcgttcg ggttccgggg ccctgggtca
     4021 tatgaccggc cgcatttcat caaagtcgtc ggagcgatgc gagacgtgga cgaactgaca
     4081 ctcgacggaa tcacgggctt gggttcttcc atccaggcaa tcctgtcgcc gatctggctg
     4141 gatgaacaat ggcagttgct gaaagatctg gaagacgtgc gcgacaacgc ggccgcgatt
     4201 gccgagatga aggaaatcat gagcggtccg ctgtggccag ttgaagaaaa gccgaaagcg
     4261 gggcgcaggc gtcgcagttt catggccagc gaacttcgga agcccgacga caacggagtg
     4321 ttcaggctca tcggggtcat ggtggtatcg aagaccaagg gaacgtttcg cccgaacctg
     4381 ggttatggat agccagggag cgttacgacg cacagtccac cctggacgaa taagaccgta
     4441 aacggaatgc cgcggtgtct cgaattttgt ctaatctgga tcagactaat cgtgagtaga
     4501 caaaggcggc gcgacggcat tctttcccgg attttccccg ggatgatcag gcagccatcg
     4561 gtgtcggccc ggtcaacccc ggtctgtaag ccattgccac agcgggatca tctggatcct
     4621 ggcggt
//
LOCUS       DAOPPA010000249         4610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20320_length_4610_cov_4.367069, whole genome shotgun sequence.
ACCESSION   DAOPPA010000249 DAOPPA010000000
VERSION     DAOPPA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20320_length_4610_cov_4.367069"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKH54_08400"
     CDS             complement(<1..963)
                     /locus_tag="PKH54_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOC99953.1"
                     /translation="MRSASSSRIASLGGSVATAMALALGTLAPLSATCQSTFDEAADP
                     VDALAADSSDERIAELESNGIDINAADIGELAEVPGISRSDAAILLERRGKAGSFASI
                     EEAASTEGLSPTAVEALHDFGRCRIDSKTEYWIRISSAGAWHSNTLKRGANIMKMPDF
                     TGGIAAGANGRWIVWAGVRTTPGASSIHAQEGQLVATGPGVAASAPAFGLHYDGGWYS
                     VTAGTFKVGFGNGLTMDITGLKRPSGHKPCLSAVMSAASGSIYPMRHFTGAAASFRKA
                     VGRDGWIVAGTVFASYRLTDPTLSDVTWNRCPDLTSECADGDKAP"
     gene            1118..3400
                     /locus_tag="PKH54_08405"
     CDS             1118..3400
                     /locus_tag="PKH54_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOC99954.1"
                     /translation="MSRTIGVMRSSAVHRLFLAFVALSVAGCEMVGEGGDPSGLSSPI
                     INGKAETGWPAVGALVMSYPFYGFTGSFCSGTLIDRRWVLTAAHCVSPSDEFPVQPGI
                     VSFMITSDARPTSSGARPAGVLVQADYFVTHASYNGQNTDYDIALVHLSRDVTEADPI
                     PHNTASLSTSLEGKSFFFVGFGVDNGAAETGGGLKRSVNIPIADINARHFISDAYNTA
                     VCFGDSGGPALYPMGGVQKVVGVNSAVSGYGSDPCIGVGYHVRVDAHSTWIETVMNTY
                     PPDCRNKPSACLCAAACKSDGTCDESACETRSCGEAVDCIFDCYGSGDVESCGIGCFV
                     SGPEAAREGLDNLMGCYVTYCYPKSIPWDNCVADTCNGKYNACYLADREKLDCPGVYD
                     CWGDCLTPVCSKSCEWLAMEGVSELWAPLASCIASNCADVYGSALGSCISKNCPSQTN
                     GCLDPDECDPRGGDCPDGTKCRQFMEHSTTCFESQGLGLGEKCKIESGKPDPCDDGLA
                     CMSGEGGRLCRKVCSKAEGCAEEGEVCKGLPLPSLPEWGWCGCVDLDEDTVCVDDDCN
                     DTDSSVFPGAREWCDGLDNNCDGTTDEGCSTSCPDADSDGSCDDADCDDENDSVRPGA
                     VEVCGDKLDNDCNGATDDGCSGCIDLDKDGYCSDRDCNDRDYRISPKAQEVCDGIDNN
                     CDGATDEGCAIGGEDVIEQDTTAGDDDQGGITTLDGGCSAGSGGSGTSHLLILAAIVM
                     LAACRRVFKFRARRSGGLLG"
     gene            3393..4034
                     /locus_tag="PKH54_08410"
     CDS             3393..4034
                     /locus_tag="PKH54_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H1/viroplasmin domain-containing protein"
                     /protein_id="HOC99955.1"
                     /translation="MGKKKPKYYAYFLPESRTRGIVESWDDCLAATSGSSARYRGFDS
                     RGAALEWLDAGAVYEDRKAAKTLAQADLPEDAVFFDSGTGRGQGTEVNVTDRDGTPLA
                     FMVAPSNRVTTFGTVLLSEGRTNNYGELMGCYLAIKVAMKTGRRLVMGDSALVLEYWS
                     RGRVNREAAGKDPDLAKLVKATAELRGSFEAAGGRLEHVPGGINPADLGFHRD"
     gene            4196..>4610
                     /locus_tag="PKH54_08415"
     CDS             4196..>4610
                     /locus_tag="PKH54_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99956.1"
                     /translation="MQARHLLSFFVVTAMLPCVGCGDGAGSEDAVDHGLIDISGADTA
                     DEVTADHGSDYGRDSAADSIVVPDVADVGEPVDAVDDPGFGDAPPEICRNPVKWDPSN
                     ALVKVFSDVTDQSGLLSQKVRGIRAGSVDFDGDGLP"
BASE COUNT          836 a         1371 c         1470 g          933 t
ORIGIN      
        1 cggagccttg tcgccatcgg cgcattctga cgttaagtcg gggcaacggt tccaggtaac
       61 gtcggacaaa gtcggatccg tcaaacggta cgacgcgaag accgttccgg cgacgatcca
      121 gccatcccgg ccgaccgcct tccggaagga cgcggcagca ccggtgaagt ggcgcatggg
      181 gtagatcgaa ccggacgccg ccgacatcac tgcggacagg cagggcttgt ggccgctggg
      241 gcgcttcagc ccggtgatgt ccatcgtaag gccgttcccg aatccgacct tgaaagtgcc
      301 ggccgttacg gaataccagc cgccgtcgta atgaagcccg aacgccggcg cggacgcggc
      361 gactcccggc ccggtcgcca ccagttgtcc ctcttgcgcg tgaatcgacg atgcccccgg
      421 ggttgtgcgc acgccggccc agacgatcca ccgaccgttt gcgccggcgg caattccgcc
      481 cgtgaaatcg ggcatcttca tgatattcgc gcctcgcttc agggtgtttg aatgccaggc
      541 gccagcggat gaaatcctta tccagtattc ggttttggaa tcgatccggc accgaccgaa
      601 atcgtgaagc gcctccaccg ccgtcggaga gagcccttcc gtggacgccg cttcttctat
      661 cgaggcaaag gagccggcct taccccgtcg ttcgagcagg attgcggcgt ccgatcgcga
      721 gattcccggc acttcggcca gttcaccgat atcagccgcg tttatatcga ttccgttcga
      781 ttcaagctcc gcgatccttt cgtcgctgga atccgcggcc agcgcgtcaa ccggatccgc
      841 cgcttcgtcg aaggtggact ggcaggtggc ggacaggggc gccagggttc ccagggccag
      901 tgccatggca gttgccacag acccgcccag gcttgcgatc ctgcttgatg aggcgctgcg
      961 catggccgta tatcggcccc gggacgatgt tctttcacgg gatttgcaat gtcatcgaat
     1021 ctgttgacga aagacagtga ctgacgcacc atagaccggt ttgtccgcga ccccgtttcg
     1081 tggcccggat tccattcgaa ttgcagaggt gcccctaatg tcaagaacta tcggcgtcat
     1141 gaggtcttcc gcggtccatc gactgtttct ggcgttcgtc gccttgtctg tcgcgggttg
     1201 cgagatggtt ggggagggcg gggatccgtc ggggctgtca agtccgatca tcaacggcaa
     1261 ggccgagacc gggtggccgg ctgtcggcgc gcttgtcatg tcctatccgt tttacggttt
     1321 caccggttcg ttctgttcgg ggaccctcat cgatcgtcgc tgggtgctga ccgccgccca
     1381 ctgcgtgtcg ccgtccgacg agttcccggt tcagcccggc atcgtgtcct tcatgattac
     1441 aagcgatgct cgtccgacat cctccggggc aaggccggcc ggcgtgcttg tccaggccga
     1501 ttactttgtc acccatgctt cctacaacgg ccagaacacc gactacgaca tcgcgctggt
     1561 gcacctgtcc cgtgacgtca ccgaggccga tccgattccg cacaacacgg cctcgctttc
     1621 gacgtccctt gaaggcaagt cgtttttctt cgtcgggttt ggcgttgaca acggggctgc
     1681 cgaaaccggc ggcggcttga agcgttcggt caacatcccg attgccgaca tcaacgccag
     1741 acatttcatt tcggatgcct acaacactgc ggtctgcttc ggtgattccg gcggtccggc
     1801 cctttatccg atgggtggcg tgcagaaggt ggttggcgtc aattccgccg tttccggtta
     1861 cggttcagat ccctgcatcg gagtcggtta ccatgtgcgc gtcgatgccc attccacgtg
     1921 gatcgagacg gtcatgaata cgtatccgcc ggactgcagg aacaaaccgt ccgcgtgcct
     1981 ttgcgcggcc gcctgcaaga gcgacggtac ctgtgacgag tccgcctgtg aaaccagatc
     2041 ctgcggcgag gccgttgact gcattttcga ctgttacggt tccggcgacg tcgagtcttg
     2101 cggcatcggc tgctttgtct cgggtccgga agcggcccgc gaaggcctgg acaacctgat
     2161 ggggtgttat gtcacctact gctatccgaa gtccataccg tgggacaact gcgtcgccga
     2221 cacgtgcaat ggcaaatata acgcttgtta cctcgccgac cgcgaaaagc ttgactgccc
     2281 cggcgtgtac gactgctggg gcgattgcct gaccccggtc tgctcaaaat cgtgcgaatg
     2341 gctggcgatg gaaggcgtgt cggagctatg ggccccgctt gcatcctgca tcgcctcgaa
     2401 ttgcgcggac gtttatggat ccgccctggg ttcatgcatt tcgaagaatt gcccctcgca
     2461 gaccaacggc tgcctggatc cggacgaatg cgatcccagg ggcggcgact gtcccgacgg
     2521 aacgaaatgc cgccagttca tggagcattc gacaacctgc ttcgaaagtc aggggctcgg
     2581 acttggggag aagtgcaaga tcgaaagcgg caagccggat ccgtgtgacg atggactcgc
     2641 ctgcatgtcg ggcgagggtg gccgcctctg ccgcaaggtc tgttcaaagg ccgaggggtg
     2701 cgcggaagaa ggggaagtct gcaagggcct tccgcttcca agtcttcccg aatggggctg
     2761 gtgcggatgc gttgatcttg atgaagacac cgtctgcgtg gatgacgact gcaacgatac
     2821 cgactcctcg gttttccccg gggcgcggga gtggtgcgac ggtcttgaca acaactgcga
     2881 cggcacgacc gacgaagggt gttcgacatc gtgtccggac gccgattccg acggatcgtg
     2941 cgacgacgcc gattgcgacg acgaaaacga ttccgtgcgt ccgggggcgg tcgaggtatg
     3001 cggagacaaa ctggacaacg actgcaacgg cgcgaccgat gacggatgct ccgggtgcat
     3061 cgatctggac aaggacggat actgttccga ccgcgactgc aacgacaggg actacaggat
     3121 cagtcccaag gcgcaggaag tctgcgacgg catcgacaac aactgcgacg gcgcgaccga
     3181 tgaagggtgc gcgatcggcg gcgaagacgt gatcgagcag gacacgaccg ccggcgacga
     3241 tgatcagggc gggatcacga ctctcgacgg cggctgttcg gccggttccg gcggcagcgg
     3301 cacctcgcac ctgctgatcc ttgccgcaat cgtcatgctg gcggcgtgcc gtcgtgtctt
     3361 caagttccgc gcgcgtcgtt ccgggggcct tcttgggtaa gaagaagccg aagtactacg
     3421 catatttcct tcccgaatcc cgtacccgcg gcatcgtgga gtcgtgggat gactgccttg
     3481 ccgcgacgag tggaagcagt gcaaggtaca ggggcttcga ttcgcgcggt gcggccctgg
     3541 agtggcttga tgccggagcg gtctatgaag acagaaaggc ggccaagacg cttgctcagg
     3601 cggatcttcc ggaagatgcg gtcttttttg attcgggtac cggccgtggc cagggaaccg
     3661 aggtgaacgt taccgatcgc gacggaaccc cgcttgcctt catggttgcg ccttctaacc
     3721 gtgtcacgac cttcggtacc gtgctgcttt cggaaggccg gacaaacaat tatggcgagc
     3781 ttatgggctg ctatcttgcc atcaaggttg cgatgaaaac cggccgcagg ctggtgatgg
     3841 gcgacagcgc cctcgttctt gaatactggt cccggggcag ggtgaaccgg gaggccgccg
     3901 ggaaggatcc cgatcttgcg aaacttgtga aggccactgc cgaactcagg gggtcattcg
     3961 aagcggctgg tggaaggctt gagcatgtcc cgggcggaat caatccggcc gatctgggct
     4021 ttcaccgcga ctgacttgac gctcgtccgg gatgcgcccg aggtcgttta ccgtccacgg
     4081 cagttgaaga agttggtcga tccgcccatt gttggagctt cacaggcctt cgatccgggt
     4141 ggataatctg cccgcgcccc ggatcaggcc ggaaatcatc caggagaaga ccttgatgca
     4201 ggcacgacat ctcttatcgt ttttcgttgt gaccgcaatg ctgccatgcg tcggctgcgg
     4261 cgacggggca ggttcggaag atgcggtgga tcacggcttg attgacattt cgggcgccga
     4321 cacggccgac gaagtcacgg ccgatcatgg cagcgattat ggacgggatt ctgccgctga
     4381 ctcgatcgtt gtccccgatg tcgcggatgt cggggagccc gtcgatgcgg tcgatgatcc
     4441 cggtttcggg gacgcccctc ccgaaatctg caggaaccct gtcaagtggg atccctcgaa
     4501 cgcgctcgtg aaggtttttt cggacgtcac cgaccaatcc ggccttctgt cccagaaagt
     4561 ccgtggcata cgcgcggggt cagtcgattt cgacggcgac ggcctgccgg
//
LOCUS       DAOPPA010000250         4609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20323_length_4609_cov_4.272288, whole genome shotgun sequence.
ACCESSION   DAOPPA010000250 DAOPPA010000000
VERSION     DAOPPA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20323_length_4609_cov_4.272288"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..691)
                     /locus_tag="PKH54_08420"
     CDS             complement(107..691)
                     /locus_tag="PKH54_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOC99957.1"
                     /translation="MKVLAINGSSRKGGNTEILLKAVLAPVEAAGIETEIYQIGGKLL
                     HGCTGCRSCRERNESTCIFTDDVMNDVIQKMIQADAIVIGSPVYFSDLSSEIKALIDR
                     AGYCTLGRRLLRRKVGAAVAAVRRGGAVHTVDSINHFFMISEMVIPGSTYWNFGMGGP
                     AGAVVEDAEGLRNMQNLGENIVWLLRHTSEFRDR"
     gene            complement(702..1364)
                     /gene="upp"
                     /locus_tag="PKH54_08425"
     CDS             complement(702..1364)
                     /gene="upp"
                     /locus_tag="PKH54_08425"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOC99958.1"
                     /translation="MDLSRFPNLHLLTDIFPNQVKGLMTIIRDRETDRDRFIFYADRL
                     IRLLVERSLEFLPVAPESVVTPTDATYAGARFLGQICAVSVLRAGESMEMAVRDVCGK
                     VRLGKILIQRDEETAEPHLYYSKLPPDISDRWVLLLDPMLATGGSIIKAIEIIKEKGV
                     PEDRIIFVNLVSALFGLNRVFSRFPQIRIVTAEVDPELNDRCYIVPGLGDFGDRYFGT
                     ER"
     gene            1533..2414
                     /gene="folP"
                     /locus_tag="PKH54_08430"
     CDS             1533..2414
                     /gene="folP"
                     /locus_tag="PKH54_08430"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC99959.1"
                     /translation="MYALEDKTANIAAAAADGTRPPLLMGILNVTPDSFSDGGDFVNP
                     RAAIDRALQMVEQGADIIDVGGETTRQGAVAVPEPVELSRVIPTVRAITSATEVPVSI
                     DTRKASVAAAAMDAGACIVNDVSGFRFDPGMIPLLGRRRPVAVAMHMRGTPADMQQHA
                     AYASLTGEIIAELMPQLQAVFQAGLPRERLWIDPGIGFSKTATQCAELLSGIGVFAAL
                     GFPVLVGPSRKSFIGAITGRTEPKSRIFGTAAAVAAAVINGARIIRVHDVAEMRDVIK
                     VARAIARNVPTETEARL"
     gene            2411..3217
                     /gene="cdaA"
                     /locus_tag="PKH54_08435"
     CDS             2411..3217
                     /gene="cdaA"
                     /locus_tag="PKH54_08435"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOC99960.1"
                     /translation="MNPFSDIFLDLSKAALISSIVDITIVFYIIYRVMLLIKGTRALQ
                     MLIGVFLTLGLYLISNDAVLGLTTLHWILEKFVSYFLVILIILFQPDIRRALSQVGRN
                     PLFLTGNRKAGTAVYEEVIKAAMRLSDKRLGALIVLEKEADLTPFTEEAIAMDAQVTA
                     DTLFSIFIPTHENPLHDGAVIIREGRISHAACFLPLTENQKIDKNLGTRHRAAIGLTE
                     ATDAVVVVVSEETGTVSICDHGEIRRGFDARSLRDALMDLFGPGRRKGHE"
     gene            3214..4281
                     /locus_tag="PKH54_08440"
     CDS             3214..4281
                     /locus_tag="PKH54_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOC99961.1"
                     /translation="MTMNENFNNEGKDEAKTIPEMPATRPRTQKMPARRGLLFKLVRE
                     NLGIKVLSLILAVALFTLVRTERGNEAEIDIPVILTNISEDQVFVGTMPSSIKVMVRD
                     RWSRLTKALERKASPYGVDLRGFKNGSVFVFDREKIRQAIGVEGLYIHSIYPSEFTVN
                     MEPNVEITVPIKLNPVGKASTGYTVPTEEARLTPSEVSIKGAKSSVSPIRELATYPVD
                     ISSLKRDSVIEVQIQKPSSKFLSLNVDRVRVEIPVQEIQSQKVFDQVPVKIRNCPDDY
                     VCSVVPELVDVTLSGSLPTIFDVQEKRLPLEIYVDASDFDPRVSGHPGIRPACERPAG
                     LKCTESPKSVNLTIQKETAAR"
     gene            4340..>4609
                     /gene="glmM"
                     /locus_tag="PKH54_08445"
     CDS             4340..>4609
                     /gene="glmM"
                     /locus_tag="PKH54_08445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676942.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOC99962.1"
                     /translation="MADTERKFFGTDGVRGIANVYPLVPEVAMALGRAIAVMFPGGQA
                     RTRILIGKDTRISGYMFESALATGALSMGADVLFVGPIPTPGIAYL"
BASE COUNT         1003 a         1366 c         1328 g          912 t
ORIGIN      
        1 accagataca tcaggtaggc cgccaggaat accggcccaa ggaagaagac cagcatcctg
       61 accgtgtcca gacccccggc ggcaagcacc gacagaacgg tttccatcac ctgtccctga
      121 attcagacgt gtgacgcaga agccagacga tgttttcgcc aaggttctgc atgttgcgca
      181 gcccctcggc atcctcgacg accgcgccgg ccggtccgcc catgccgaag ttccagtatg
      241 tcgaacctgg aatcaccatc tcggaaatca tgaagaagtg gttgattgaa tcgacggtgt
      301 gaaccgcacc gccgcgcctg accgcggcga cggccgcgcc aaccttgcga cgcagcaggc
      361 ggcgcccgag cgtgcagtat ccggcacgat cgataagggc ctttatttca ctgctcaaat
      421 ccgaaaaata gaccggcgat ccgataacga ttgcatcggc ctgaatcatc ttttgaatga
      481 catcgttcat cacgtcatcg gtaaagatgc aggtcgattc gttccgttcg cggcaggatc
      541 tgcacccggt gcagccatga agcagctttc cgccaatctg gtaaatctcg gtctcgattc
      601 ccgcggcctc gactggcgcc aacaccgcct tcaacaggat ttcggtattt ccgcccttcc
      661 tggaacttcc attgattgca agaaccttca tcttcgcccc cttacctttc ggtcccaaag
      721 tatcgatccc cgaaatcgcc cagccccgga acaatatagc agcggtcgtt cagctcggga
      781 tcaacctcgg ccgtgacgat ccggatttgc gggaacctgg aaaagacccg attcaatccg
      841 aaaagcgcgg aaaccaggtt cacaaaaatg atgcgatcct cgggaactcc cttttccttg
      901 atgatttcga tggccttgat aatcgagccg ccggttgcaa gcatcggatc aagcagcagc
      961 acccatctgt cgctgatgtc cggcggcagc ttggaataat aaagatgagg ctcggccgtc
     1021 tcctcgtcgc gctggatcag tatctttccc agccgcacct ttccgcagac gtcgcgaacc
     1081 gccatttcca tgctttcgcc ggcccgaagc accgagaccg cgcagatctg gccaaggaag
     1141 cgtgccccgg cgtatgtcgc gtcggtcggc gtcaccacgc tttccggggc aacgggcagg
     1201 aattcaagcg aacgctcgac aagcagccgg atcaggcgat ccgcataaaa gatgaaacgg
     1261 tcgcggtcgg tttcgcgatc cctgatgatc gtcatcaagc ccttgacctg gttgggaaat
     1321 atgtccgtca gaagatgaag gttcgggaat ctggaaaggt ccatttttca cctggtggct
     1381 taatgaccga aggcgccggc gccggaatcg cggctgcccg tcccggccag tatatcgaca
     1441 acgaaagata ccagcattgc ggcacggcgt gaaaccccat tatttcgcgc gcgcgtttgt
     1501 taagcacacg ggaacggacg tatgattggg ccatgtacgc ccttgaagac aagaccgcga
     1561 acatcgccgc cgcagcagcc gacgggacgc gtccacccct gctgatggga atccttaacg
     1621 tcactccgga ctcgttttcg gacgggggcg atttcgtcaa cccgcgcgcc gcaatcgacc
     1681 gcgcccttca gatggtcgaa cagggtgccg acatcatcga cgtcggcggc gaaacgacaa
     1741 gacagggggc cgtcgccgtt cccgaaccgg tcgagctttc acgcgtaatt ccgaccgtgc
     1801 gcgcaatcac gtccgcgacc gaagtgccgg tttcaatcga cacacgaaag gcctcggtag
     1861 ccgcggccgc catggacgcg ggcgcatgca tcgtgaacga cgtgtcgggg ttcaggttcg
     1921 acccgggaat gatcccgctt cttggacggc gccgcccggt tgccgttgca atgcacatgc
     1981 gcggcacccc ggcggacatg cagcagcacg ccgcatacgc ctcgctgacc ggtgaaatta
     2041 ttgccgaact catgccgcag cttcaggcgg tgttccaggc cggacttccc cgcgagcgcc
     2101 tgtggatcga tcccggaatc ggattttcaa agacggcaac gcagtgcgcg gaattattgt
     2161 ccggaatcgg tgtttttgcg gcgctcggct ttcccgtgct tgtcgggccg tccaggaagt
     2221 cgttcatcgg ggcgatcacc ggacgaaccg aaccgaaaag tcgtatattc gggactgctg
     2281 cggcggttgc ggccgcggta atcaacggcg caaggatcat ccgggttcac gatgtcgcgg
     2341 aaatgcgcga cgtgatcaag gttgccaggg caattgcccg aaacgttccg actgaaacgg
     2401 aggccagact ttgaacccgt tctcagacat attcctggat ttgagcaagg cggcgctgat
     2461 atcgtcgatc gtcgatatca ccatcgtctt ttacatcatt taccgcgtga tgctgcttat
     2521 caagggaacc agggcattgc agatgctcat cggcgtcttc cttacgcttg gtctttacct
     2581 gatctcgaac gatgccgtgc ttggactgac cacactgcac tggatcctgg aaaagttcgt
     2641 cagctacttc ctggtcatcc tgatcatcct gttccagccc gacatcaggc gcgccctctc
     2701 gcaggtcggc cggaacccgc tgttccttac cggtaaccgc aaggccggaa ccgccgttta
     2761 cgaagaagtt atcaaggcgg cgatgcgcct gtcggacaag cggctgggcg ctctgatcgt
     2821 cctggaaaag gaagcggatc tgacgccgtt caccgaggaa gcaatcgcca tggacgctca
     2881 ggtgacggcg gacacactct tttccatatt cattccgaca cacgaaaacc cgcttcacga
     2941 cggcgccgtg atcatccgtg aaggccgcat cagccacgcc gcatgtttcc tgccgttgac
     3001 ggaaaaccag aagatcgaca agaacctggg caccaggcac cgcgcggcca tcggactgac
     3061 cgaggcaacc gatgcggtcg tggttgtcgt gtccgaggaa accggcaccg tctcgatatg
     3121 cgatcacggt gaaatcaggc gcggattcga tgcgcgttcc ctgcgcgacg cgctgatgga
     3181 cctgttcggg cccggacggc gaaaggggca tgaatgacga tgaacgaaaa cttcaacaat
     3241 gaaggaaagg acgaggcgaa gacgattccc gagatgccgg ccacaagacc aagaacccag
     3301 aagatgccgg ccaggcgcgg actcctgttc aagctggtca gggaaaacct gggaatcaag
     3361 gtgctgtcgc tgattctggc ggtggcgctg ttcacccttg tgcgcactga acgcggcaac
     3421 gaggccgaaa tcgacattcc ggtcatcctt acgaacattt ccgaagacca ggtgtttgtg
     3481 ggaacgatgc cctcgtcaat caaggtaatg gtgcgcgaca ggtggtccag gctgacgaag
     3541 gcgctggaaa gaaaggcctc gccgtacggc gttgaccttc gcggattcaa gaacgggtcc
     3601 gtattcgttt ttgaccgcga aaagatccgg caggcaatcg gggtcgaggg cctttacatc
     3661 cattcgattt atccgtccga attcacggtc aacatggaac cgaatgtcga aatcacggtt
     3721 ccaatcaagc tgaacccggt cggcaaggct tcgacgggat acacggtccc gaccgaggaa
     3781 gcccgcctta ctccatcgga agtctcgatc aagggcgcca agtcgtcggt ttcgccaatt
     3841 cgtgagctgg cgacatatcc cgttgacatc agttccctga aacgggattc ggtgattgaa
     3901 gtccagatcc agaagccatc atcgaagttt ctttcgctga atgtcgatcg cgtgcgggtt
     3961 gaaataccgg tccaggagat tcaaagccag aaggtgttcg accaggttcc ggtcaagata
     4021 cgcaactgcc cggacgacta tgtttgttcg gtcgttcccg aactggttga cgtcacgctt
     4081 tcaggttccc tgccgacgat attcgatgtg caggaaaagc ggctgccgtt ggaaatttac
     4141 gtcgatgcgt cggatttcga tccaagggta tcggggcacc cgggcatccg ccctgcgtgc
     4201 gagcgtccgg ccggcctgaa gtgcaccgaa tcgccgaagt ccgtgaacct gaccatccag
     4261 aaggaaacgg cagcgcgttg aaatgatgaa gtatcgtttt caggtgccgg cgaaggccgc
     4321 gccatgtcag gagtcttgaa tggccgatac tgaaagaaag tttttcggga ccgatggcgt
     4381 gcgcggaatc gccaacgtgt atccgctggt tccggaagtc gccatggccc tgggacgggc
     4441 gatcgccgtc atgtttccgg gcggccaggc caggaccagg atcctgatcg gaaaggacac
     4501 caggatttcc ggatacatgt tcgaatccgc ccttgcgacc ggggcgctct cgatgggcgc
     4561 cgacgtgctt ttcgtgggcc ccatcccgac accgggcatc gcatacctg
//
LOCUS       DAOPPA010000251         4554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20644_length_4554_cov_3.328295, whole genome shotgun sequence.
ACCESSION   DAOPPA010000251 DAOPPA010000000
VERSION     DAOPPA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20644_length_4554_cov_3.328295"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKH54_08450"
     CDS             <1..454
                     /locus_tag="PKH54_08450"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC99963.1"
                     /translation="KTRDSLEDAIRKEMETFVDYQELGRLTLSDEWDKLKKPERKRFI
                     DGFKKMVQRSYVKKFNPDTPFEVEIGSSTEKEGRITVSSVIRSGKSEAEVSYAFHKSA
                     DRWMVHDVIIDKVSMVKNYRKQFTSIIGKEGFNGLMARIEKKNAARAD"
     gene            complement(561..3038)
                     /locus_tag="PKH54_08455"
     CDS             complement(561..3038)
                     /locus_tag="PKH54_08455"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOC99964.1"
                     /translation="MKKAFIFAALIFAVPFLVSCGSESVQNDVSGSDVAFDAVADALG
                     DNGHTDDVVVDAGRDVLDPDAVSDADVMGQDLIGDIAEDVPISCPGNLWCPCKDNNQC
                     YSNYCVETSGGKVCSKNCGGGGDCPRGWSCTQLVVSGDATFICTDPFARACRPCTRDS
                     DCVPGFGASESEVYDCIPYGPGGSYCGGSCAGEDDCAEGFVCNPNPDGSGQRCMFAEG
                     ECPCSQGFIDQGYLTECYVENEYGRCKGTRTCDAECSASQPQAESCNLVDDDCDGATD
                     EGIAGTACVNENQEGSCPGTAVCSNGVFSCSGQTPQREFCDAVDNDCDGLTDEDASNC
                     RNFFKDGDDDGFGLSADFQCLCQASETYSAAVGNDCNDNDVNVYPGVIESCNGKDDNC
                     DGVTDEMGAIGCTSYYLDVDNDTYGVESDKKCLCGPSGDYRATRGGDCADDNPLVSGG
                     TAESCNGLDDDCDGQTDEENSLGCSNWYRDGDGDSFGSMTDYKCLCSSTVVYKVDVGG
                     DCNDNNLAINPAATETCNGVDDNCDLITDDEGASGCTPYYLDNDGDQYGVGTPKCLCA
                     PFNKYTALVDGDCSDSDGSANPGMTEVCNNKDDDCDGVKDEPGATGCTAYFLDTDRDS
                     YGVTDNVACLCAVTGDYRATRGGDCDDANELINPGIAEKCDGLDNDCDKTVDEDGAVG
                     CTGYYLDSDRDGYGDVANYRCLCAALAGSYDVTNASDCCDIDNLAYPGANPTVYYVGP
                     TKCATTGREWDYDCSGTNEKEDARVNGGCEGWAVGDGCDREYGWRSSVAPACGVTAEF
                     LTGGCGYTGIPVDCKDPVFSNRTQKCH"
     gene            3237..4520
                     /locus_tag="PKH54_08460"
     CDS             3237..4520
                     /locus_tag="PKH54_08460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOC99965.1"
                     /translation="MDDVIALILGGGQGTRLYPLTLQRSKPAVAVAGKYRLIDITVSN
                     CINSDIRKVFVITQFLSASLHHHIMHTYRFDAFSKGFVEILAAEQTPNRSTWYQGTAD
                     AVRESLRHIKDFQNEAIVILSGDHLYRMDYRDMVQRHLNMDADITLAVQPVPKHEASR
                     MGLLKAGPDGRVVDFFEKPKQEDILERFRCPAETIREHGLEGDEWYLGSMGIYVFKPS
                     VLEELLSDPTRMDFGREVIPGSISTHKVLAHPFTGHWEDIGTIRSYFDAHIAMTRENP
                     PFPIYEPGKPIYTRARNLPPAVVMRSKIKNCLFSEGSVVTGAVVRESIIGVRSIVRPE
                     ARLTQVVMLGADYYEGERAPAIYGDFQGELPPMGIGHDTTLEKCIIDKNARIGNFVTI
                     TAKEDGVNMQGPNYWVCDGITVIPKNAVIPDGSII"
BASE COUNT         1021 a         1338 c         1230 g          965 t
ORIGIN      
        1 caagaccagg gactcgctcg aggacgccat ccgcaaggaa atggagactt tcgtcgatta
       61 tcaggaactt ggccgcctga ccctttccga tgaatgggac aaactgaaga agccggaacg
      121 caagcggttt atcgacggct tcaagaagat ggttcagcgc agctatgtca aaaagttcaa
      181 cccggatacg cctttcgagg ttgaaatcgg ttccagcacg gaaaaggaag gccggattac
      241 ggttagttcg gtaatccgct cgggaaagtc agaggccgaa gtcagctacg cattccacaa
      301 gtccgccgac cggtggatgg tccatgacgt gatcatcgac aaggtcagca tggtaaaaaa
      361 ctaccgcaag cagttcactt caataatcgg caaggaaggg ttcaacggcc tgatggccag
      421 gattgaaaag aagaacgctg cccgggccga ctgattcgcc gaattcctga ccgttacttc
      481 ggaaacttgc ttgaaaggtt caaccgccgg tcaaagagtc atggggataa gcattcagtt
      541 cgtgaaggca aactcacgga tcagtggcac ttctgggtgc ggttgctgaa cactggatcc
      601 ttgcagtcaa ccgggattcc ggtgtaaccg cagccaccgg tcaggaactc tgccgtgaca
      661 ccgcaggcgg gggcaaccga cgacctccag ccgtactcac gatcacatcc gtcgccaacg
      721 gcccacccct cacagccgcc attgactcgg gcatcttcct tttcgttggt gcccgaacag
      781 tcatagtccc actcccggcc ggtcgtggcg cacttcgtcg ggccaacgta atagacggtt
      841 ggattcgcgc cgggatacgc caggttatca atgtcgcagc agtcgctggc gtttgtcacg
      901 tcatagcttc cggcaagggc cgcgcacagg catcgataat tcgccacgtc gccgtatccg
      961 tcgcgatccg aatcgagata gtatcccgtg cagccgaccg cgccgtcttc atcgacagtc
     1021 ttgtcgcaat cgttgtccag gccatcgcat ttttcggcaa tgcccggatt gatcagttca
     1081 ttcgcatcgt cgcagtcccc gcccctggtg gcacgataat cacccgtcac tgcacaaaga
     1141 caggctacgt tgtcggtaac gccatagctg tcgcgatcgg tatccaggaa gtatgccgtg
     1201 cagccggtcg cacccggttc gtccttgacc ccgtcgcaat cgtcgtcctt gttattgcag
     1261 acctcggtca tgccaggatt cgcggaaccg tccgaatcgc tgcagtcgcc gtccaccagc
     1321 gcggtgtact tgttgaacgg cgcgcagagg cactttggcg tgccgacgcc gtactggtcg
     1381 ccgtcattgt caaggtaata cggggtgcat ccactggcgc cttcgtcatc ggtgatcagg
     1441 tcgcagttat catcgacgcc gttacacgtc tcggtggccg ccgggttgat ggccaggttg
     1501 ttgtcgttgc agtcgccgcc aacatcgacc ttatagacca cggtgctcga gcacaggcac
     1561 ttgtaatccg tcatggatcc gaacgaatcc ccgtcaccgt cgcgatacca gttcgagcag
     1621 ccgagggaat tctcttcatc tgtctggcca tcgcagtcat catccagccc gttgcacgac
     1681 tcggcggtgc caccgctgac cagcggattg tcgtcggcac aatcgccgcc cctggttgcc
     1741 ctgtagtcgc cggaaggtcc gcaaagacac ttcttatcgc tttcaacgcc gtaggtgtcg
     1801 ttatcgacat ccaggtaata actggtgcaa ccgattgcac ccatctcgtc ggtcacgccg
     1861 tcgcagttgt catccttgcc gttgcaggac tcgatgacac ccggataaac gttgacgtcg
     1921 ttatcgttgc agtcattacc aacggccgcc gaataggttt ccgacgcctg gcacagacac
     1981 tggaaatcgg cactcagccc gaagccgtcg tcatcgccat ccttgaaaaa gttacgacag
     2041 ttgctggcat cctcgtcggt cagtccatca cagtcgttat cgaccgcatc gcagaattcc
     2101 cgctgggggg tctgtccgct gcacgaaaaa actccattcg aacatacggc ggttccggga
     2161 caagagcctt cctgattttc gttcacgcat gcagtcccgg cgatcccctc gtcggtggcg
     2221 ccgtcacaat cgtcgtccac caggttgcag gattcggcct gcggctggct tgcactgcat
     2281 tccgcatcgc aggttctcgt tcccttgcag cgaccatatt cgttttcgac atagcattcg
     2341 gtcaggtatc cctgatcgat gaagccctgc gagcacggac actcgccctc ggcgaacatg
     2401 caccgctgac cggaaccatc gggattcgga ttgcagacga atccttccgc gcaatcgtcc
     2461 tcgcccgcac atgaaccgcc acagtagctt ccgccgggtc cgtacggaat acagtcatag
     2521 acttcagact cgctggcgcc gaaaccaggg acacagtcgc tgtccctggt gcatgggcgg
     2581 caagccctgg caaacgggtc ggtgcagatg aaagtcgcgt ccccggacac gacaagctgt
     2641 gtacaggacc agcccctcgg gcaatcacca ccgcctccgc aattcttcga gcagaccttg
     2701 ccgccggaag tctcgacaca gtagttcgaa tagcactgat tattgtcctt gcaagggcac
     2761 cacaggtttc cagggcagct tatcggaaca tcctcggcaa tgtctccaat caaatcctgg
     2821 cccatcacgt cggcgtccga aacggcgtcg ggatccagga catcacgccc ggcatccaca
     2881 accacgtcat cggtatggcc gttatcgccc aaggcatcgg cgacggcatc gaacgcaaca
     2941 tcgcttccgg aaacgtcgtt ctgcacactc tctgaaccgc atgaaacaag gaacggaaca
     3001 gcaaaaatga gggccgcaaa tatgaaagcc ttcttcatac gcattctcct gtacctgatt
     3061 cgatccggga tgacccggat ccttaaaaag agtcgcggac aggatatcag aaatgtgggc
     3121 aaatttgcca gattacgtat gatttgcgtt aatccgcaag cattgtcttt gcttgttttc
     3181 aactaaatgg tagtaagacg tttattgatc cgatttctct tcccggtggt ggaaacatgg
     3241 acgacgtcat tgccttgatt cttggtggcg ggcagggtac caggctttat ccgttgacgc
     3301 ttcaaagatc gaagccggcc gtggcggtcg ccggcaagta ccggctgatc gatataaccg
     3361 tatcgaactg catcaattcg gacattcgaa aggttttcgt aatcacgcag tttctttcgg
     3421 ccagccttca tcaccacatc atgcacacct atcgcttcga cgccttttca aagggtttcg
     3481 tcgaaattct tgcggcagag cagacaccga accgcagcac ctggtaccag ggaaccgccg
     3541 acgcggttcg cgaatcactg cgccacataa aggatttcca gaacgaagca atagtcatcc
     3601 tttcgggcga ccatctttac aggatggact atcgcgacat ggttcagcgc cacctgaaca
     3661 tggatgcgga catcacgctg gcggttcagc cggttccgaa gcatgaggct tccaggatgg
     3721 gcttgttgaa ggcgggcccg gacggacgtg tggtggattt tttcgagaag cccaagcagg
     3781 aagatatcct tgagcggttc agatgtccgg ccgagacgat ccgggagcat ggcctggaag
     3841 gggacgaatg gtacctgggt tccatgggca tttacgtgtt caagccttcc gtcctggagg
     3901 aactgctgtc agacccgacg aggatggact tcggcaggga ggtcataccc ggttccattt
     3961 cgactcacaa ggttcttgcc catccgttta ccggacattg ggaagacatc ggaaccatcc
     4021 ggtcatattt cgacgcccat atcgcgatga ccagggagaa tccaccgttc ccgatttatg
     4081 agcccggcaa acccatttac acaagggccc gcaatctgcc acccgccgtc gtcatgcgat
     4141 ccaagatcaa gaactgcctg ttttccgaag gttccgtcgt tacaggggcc gtcgtgcgcg
     4201 aatcgattat cggggtcaga tccattgtaa ggccggaagc aaggctcaca caggttgtga
     4261 tgcttggggc cgactattac gaaggggaaa gggcgccggc catctacggc gacttccagg
     4321 gtgaacttcc gcccatggga atcgggcacg acaccaccct tgagaaatgc ataatcgaca
     4381 agaacgcaag gatcggcaac ttcgtgacaa tcacggcaaa ggaagacggc gtgaacatgc
     4441 aggggccgaa ttactgggtt tgcgacggca tcacggtgat cccgaaaaat gccgtgattc
     4501 cggatggcag catcatctga accagccgct gacatacctg cgaacaagat ttca
//
LOCUS       DAOPPA010000252         4545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20690_length_4545_cov_3.379733, whole genome shotgun sequence.
ACCESSION   DAOPPA010000252 DAOPPA010000000
VERSION     DAOPPA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20690_length_4545_cov_3.379733"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKH54_08465"
     CDS             <1..637
                     /locus_tag="PKH54_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99966.1"
                     /translation="PCREPRDLDGWDARFLTVAIRQLLQVLGRINRDEIDFPDAFTQH
                     QVLVRVPEDRGGTLVWHDAIEPLEKAVKSESLPLSESELSSLERLPVREFRLELAHTI
                     LPALVKATDSDRGMFIRIILAVDGRSGKVLGFHTFDEGEFPRNLLTQVAAMLKSTGFI
                     PSQLLVNDPWVFSAIADGLPLLKSSLAVVKGMPASEDAVSAFAEVVSAGHS"
     gene            838..1158
                     /locus_tag="PKH54_08470"
     CDS             838..1158
                     /locus_tag="PKH54_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99967.1"
                     /translation="MNVSTLHSPAIVCLSMAGILLGCGGSSLPDKDVHDSVAQGLPLL
                     DSVQADDASLSDGTVFDNAGSSDLGATDDDSFDGQVTDEGAVTFPPIPVQFRTRTPTQ
                     VMMN"
     gene            1374..2069
                     /locus_tag="PKH54_08475"
                     /pseudo
     CDS             1374..2069
                     /locus_tag="PKH54_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(2261..3148)
                     /locus_tag="PKH54_08480"
     CDS             complement(2261..3148)
                     /locus_tag="PKH54_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99968.1"
                     /translation="MRHAFLVPALFLLAGCSDAGGSGDDVTDDSSVGSAIVVSGRLVQ
                     GDQFAASRPRSLAKRLSGDPLAGYQLYCVTFATPPTAAAGIADADGQVSIQIDAAGIP
                     LGCFVLDTGGIAVATLIFDDGGQTGQTVTLALGTDMGEITVDLDNGVALAPIDGDIAD
                     SAGMPCPLGTWKAAVPREDCTGTTARFTISQEPDGGYLVSFILGPVWESGPDACVDIC
                     RSGIPATVNDGTWTIEFPHDPLGCPSRMMTVAMTPNQACTEIAVESTYGPCESCSAGE
                     CGCESGTQTCTGTFTAARD"
     gene            complement(3415..3831)
                     /locus_tag="PKH54_08485"
     CDS             complement(3415..3831)
                     /locus_tag="PKH54_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOC99969.1"
                     /translation="MQIKRNKRTFWQRLLGRPVTQLPATNDFWSVSGGRIVVDLATAP
                     GLSTPGGAVRLEGKIPGRILVVHCQDGTFHAYENKCTHGGRCVDPVPGTNTVQCCSIN
                     TSTFDCDGSKIHGPARGPLKTYPVTVDAGTLSIDLG"
     gene            complement(3957..4505)
                     /locus_tag="PKH54_08490"
     CDS             complement(3957..4505)
                     /locus_tag="PKH54_08490"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC99970.1"
                     /translation="MDSVACLIKFRVVGGPDAGLEVRMDSEPVSIGRDSGCDVVLTDP
                     TVSRSHMKLERIDGRWYLQHLAPGNQTHVNGRPVIVTDPPTALKCGDRISFGASVMVV
                     EFRHTGLTDASRDPGIAADAVDGIPLDASIKLGAPPALRPPEIEPATRLPSATLRRLA
                     APVAALTLALLFLAAILLRSCE"
BASE COUNT          895 a         1265 c         1415 g          970 t
ORIGIN      
        1 tccgtgcagg gaaccacggg atttggatgg ctgggacgca aggttcttga cggttgccat
       61 tcgccagctg ctgcaggttt taggtcgcat caacagggat gaaatagatt ttccggacgc
      121 atttacccaa catcaggtac tcgtgcgtgt tccggaagat cgcgggggaa cgctggtttg
      181 gcatgacgcc attgaacctt tggaaaaggc tgtcaaatca gaaagtctgc cgctttccga
      241 gtccgagctt tcgtcgctgg aaagacttcc tgtgcgtgaa ttccgcctgg agcttgccca
      301 tacgatattg cccgcgctgg tgaaggcaac agattcggat cgcggaatgt tcatcagaat
      361 tattctggct gtggatgggc ggtctggaaa ggtgctgggt tttcacacgt ttgacgaggg
      421 cgaatttcct cgtaatttgc tgacgcaagt cgcggctatg ttgaagtcaa cgggttttat
      481 cccgtcgcaa ctgttggtta acgatccgtg ggtgttttct gcaatcgccg atggtttgcc
      541 tttactcaaa agctcacttg cggtggtgaa gggcatgccc gcgtccgaag acgctgtttc
      601 tgcttttgct gaagtagtga gtgccggcca ttcttgaaca aagggcacgg tggcgtccgc
      661 cttccgaaac cagatttttg gcgatttggt cgtctgcttc gcagagttgg aaaacggctg
      721 gaatcaggca ttgacctact taaaatcggt tctgccagaa agcgaaggct acaaggccag
      781 agcctttgtc aagccggact ttgatggtac gatttcctgc tgtaactggg ggtgcttatg
      841 aatgtttcga cgctgcactc gcctgcaata gtctgtttat ccatggcggg tattcttctg
      901 ggctgtggtg gatcgtcttt gccggacaag gatgttcacg attccgtggc gcagggtttg
      961 ccgttattgg attctgtcca ggctgatgat gcatctttgt ccgacggcac ggttttcgat
     1021 aatgccggtt cgtcggatct gggcgcgacg gatgatgatt ccttcgatgg tcaagtgacg
     1081 gatgaggggg cggtgacgtt ccctccgatc ccggttcagt ttcggacgcg aaccccgacc
     1141 caggtaatga tgaattgaat tggcatgacg ggggcggtga tgttgcttcc gatccaggtc
     1201 aggatctggc gtccgaagat tccggcaccg atgccccaac cgacgtcggc tgtattcccg
     1261 attgcagtca ggtcgagtgc ggcctggatc cagtctgtgg aaccctggat tgcggtgctt
     1321 gtgaaggtga aaattcgatc tgccgttttg gtgtctgcgt caatctggat cagatgttgc
     1381 ccgttccggc gggtcccttc atgatgggct gcaacgacca gaccgacgat tgtctggcgt
     1441 gggaaaagcc ctatcacgaa gtaaccctgg acggatttct tgtggacatt gccgaagtga
     1501 cccagcgcca gtaccagaaa tgcgtcagtg cgtctgtctg tgaaataccg gtatgtaact
     1561 gggtaccgca ttcacgtccg cattatcccg ttgtatgcgt tgactggcag aaggcaaaga
     1621 cctattgcga gtgggcgggc aagcgacttc ccaccgaggc tgagtgggaa aaggcggctc
     1681 gcggtacgga tggccgggca ttcccctgga aaaatgaaac agcaacctgt gaacaggccg
     1741 tcatgaaaga tcccgtgctt gatcttgggt gcggatcggg cgggcccgtc caggtatgca
     1801 gcagatcgtc ggtttacgac tctccgtaag ggttgtgcga catggccggc aacgtggatg
     1861 agtgggtatc cgactggtac agcgcggaat attactcggt cagcccatca acaaatccgc
     1921 cgggtcccga aagtggcact tacaagggct gtcgaggtgg ttcatcgcat gccgacgctc
     1981 cataccttcg gactttctat cgttcgtatg ttgaaccgga gctttacggc tggtccatcg
     2041 gttttcgttg cgtaaaggcc atccaatagt ccgcgcgcgc tgttcacaat tatccctgaa
     2101 gcgcctttat tccgcctggc aaagacccac atgagtgaaa aaaggccggg gctctcggtg
     2161 cattttgcct gcccccttca tggaaaattt tgaaacggaa gtctttggtg tcacccatgt
     2221 ttggaattgt cccaggatgg gaggccgaat aatgttccga tcaatccctg gccgccgtga
     2281 atgttcccgt gcaggtctgg gtgccagact cgcagccgca ttcaccggcg ctgcaggatt
     2341 cgcaggggcc ataggtggat tcgacggcga tttcggtgca ggcctggttc ggggtcatgg
     2401 cgaccgtcat catgcgggac gggcagccaa gagggtcgtg cggaaattcg atggtccagg
     2461 tgccgtcgtt aaccgttgcg ggtattccgg aacggcagat atcgacgcat gcgtcgggac
     2521 cactttccca tacgggtccc aggatgaagc tgacaagata gccgccgtcg ggttcctgcg
     2581 agatcgtgaa cctggccgtc gtcccggtgc agtcctcccg gggaaccgcg gccttccagg
     2641 ttccgagcgg gcagggcatt ccggcgctgt cggcgatatc cccgtcaatc ggcgccagcg
     2701 ccaccccgtt gtccaggtca acagtgattt cgcccatgtc ggtgcccagg gcaagcgtga
     2761 cggtctgtcc tgtctggccg ccgtcgtcaa atatcagcgt ggccacggca atacccccgg
     2821 tgtccaggac gaaacatccc agcggaatcc cggccgcgtc gatctggatg ctgacctggc
     2881 cgtcggcatc ggcgatcccg gcggcggctg tcggcggagt cgcaaacgtc acgcagtaca
     2941 actggtatcc tgccagcggg tcgccactta agcgcttcgc aagagacctg ggccgtgacg
     3001 ccgcaaactg atcaccctga accagccgcc cggacaccac aatcgcgctg ccaacggagc
     3061 tgtcgtccgt gacgtcatcg cccgatccgc cggcgtccga acatccggcc aacagaaaca
     3121 atgcaggcac caggaaggca tggcgcatga atcaccacca ggattttgta atcacaaggt
     3181 attcccggcc ggtcagtcag tcaacactcg ggccgccgtt cgccgccgac gggcacgccg
     3241 ttaagacggt tgccggggga aatagtgagc aatccacttg aatgcggtga taacgccgcg
     3301 tattcaacgc agattatgcg gcgaatagtt tgctgaagct acctatgcag ttgctggacg
     3361 gctttgatgg caagttgaca agcggaaatt gggcagaagt tgctttgccc gcggtcaacc
     3421 caggtcaatc gaaagcgtgc cggcatcaac ggtgaccgga taggtcttca gtgggccgcg
     3481 ggccgggcca tgaatcttgc ttccgtcaca gtcgaacgtg gatgtgttga tgctgcagca
     3541 ctggaccgtg ttcgtcccgg gcaccggatc gacgcagcgg ccgccgtgcg tgcacttgtt
     3601 ttcgtacgcg tggaaggttc cgtcctggca gtggacgacc aggatgcgac ctggaatctt
     3661 cccttccagc ctgaccgcgc cgcccggcgt cgagagcccc ggtgccgtgg caagatccac
     3721 aacgatccgc ccgcccgaga ccgaccagaa gtcattggtg gctggaagct gcgtgacggg
     3781 acgtcccaga agccgctgcc agaaggttcg cttgttcctt ttgatttgca tggctgacct
     3841 cgcatgaagg caatgaaagt tggtaaccga atgttggcac gatctgcctg gacaggcaag
     3901 gcatggccgc cgccgcgaac cgccagacca gaatggcgcc agcggcgccg gggccgtcac
     3961 tcgcacgaac gcagaaggat tgccgcaagg aagagcaggg caagggtcaa cgccgcaacg
     4021 ggtgcggcaa gcctgcgaag tgtggccgat ggcaaccggg tggcgggctc gatctcgggc
     4081 gggcgaagcg ccggcggcgc acccagcttg atcgatgcat caagcggaat tccatcgacc
     4141 gcgtcggccg caatgccagg gtctcgactg gcgtcggtca ggccggtgtg ccggaactcg
     4201 accaccatga cgctcgcgcc gaacgatatc cggtcgccgc acttcagcgc cgtgggggga
     4261 tctgtgacga tcaccggccg gccgttgacg tgggtctggt tgcccggcgc aaggtgctgc
     4321 aggtaccagc gcccgtcgat gcgttccagc ttcatgtggc tgcgcgaaac ggttgggtcc
     4381 gtaagaacga cgtcgcaccc ggaatcgcgt ccaatcgaaa ccggttccga atccatgcgg
     4441 acttcaagtc ccgcgtcggg gccgccgaca accctgaact tgataaggca ggcaaccgaa
     4501 tccaatggta cccccgcggc aggcagtcgc ccggccgcgc cggac
//
LOCUS       DAOPPA010000253         4529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20791_length_4529_cov_4.233572, whole genome shotgun sequence.
ACCESSION   DAOPPA010000253 DAOPPA010000000
VERSION     DAOPPA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20791_length_4529_cov_4.233572"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKH54_08495"
     CDS             complement(<1..381)
                     /locus_tag="PKH54_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99971.1"
                     /translation="MAKECRISAWAETTVGKVRKRNEDAHLILPERSFYLVADGMGGH
                     KRGDLASQLCIDSVREFLTGAGTGKTIAFFKGLLDKPFDDRAVWERSICAAIEYANRR
                     IVKAAMMNRELAGMGTTVVSAGFRG"
     gene            complement(386..2104)
                     /locus_tag="PKH54_08500"
     CDS             complement(386..2104)
                     /locus_tag="PKH54_08500"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOC99972.1"
                     /translation="MVSKSIFPAVMLLVLVAMPARSSEQQADPVMEAMLQELDRSVNL
                     LRLEAGDYDLPYFVYYAVRDVRSSFVSARNGALQSSNDGRTRMADVDIRVGSYDMDSS
                     EDKDAGFNFNQKYNPGFMGPIDDSIPALRRTLWLLSDHAYKAALMSFHNVKAKLVNSS
                     EPRKSASLSREQPVELIEEISEAEFDRPAREGHARELSAIFLQYPDIFDSGVEISAFR
                     VVRYIVNSEGTRIRTVDHYYQLLAQATVRAPDGMLLQDGVTIYGRRPGDMPDLATAAD
                     RIRTLARNLVAMKDAPVLDPATVPVLMSPEATGVFFHETIGHRLEGQRQEDEEEGRTF
                     KSYLNQAILPAFLDIFDDPSVEMFGTGSGRVSLNGFYRVDDEGVRGQKVSLVEKGVLK
                     AFLMSRKPIEGFEKSNGHGRTQGFRPAQARMANLFIQGARPMSQARLFSRFLQEMKKQ
                     NKPWGLYVETIAGGNTNTSSYGYQAFKGKPLIAYKVDAATGKKTMVRGVEIVGTPLSA
                     INRIIATSDRYGVFNGYCGAESGSVPVSTVAPEVLFSEMELQRTEDTNERPLILPPPD
                     VPAAAR"
     gene            complement(2115..2507)
                     /locus_tag="PKH54_08505"
     CDS             complement(2115..2507)
                     /locus_tag="PKH54_08505"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOC99973.1"
                     /translation="MVRVVAALVRDGTKVLITQRPANSEFGGWWEFPGGKVEPGESDG
                     EALRREISEEMGLQVDVGGIFHSNLFEYERFILDFHLYNCVILGGEIALIGISGFSWV
                     EPGELKSFRFPPADVEAVGLIAGESSAL"
     gene            complement(2545..3144)
                     /locus_tag="PKH54_08510"
     CDS             complement(2545..3144)
                     /locus_tag="PKH54_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC99974.1"
                     /translation="MESVKLPAKFALKNGVEVLVRDMVRADEPAVFEFFRLLPLEDRH
                     FLRNDVTDPIRVHNFMIDDTHDRVRAVVAELDGRIVASAEIDRNHFGSMTHIGQLRVI
                     IARELQHQGLGTILSRLLIADGVNAGIEKVLAETSTENKAAMLALEKMRFRKEATLKL
                     HVKDLAGRKHDLAVYSYDVSHIWDKMEALVADYSPMREY"
     gene            complement(3179..3733)
                     /locus_tag="PKH54_08515"
     CDS             complement(3179..3733)
                     /locus_tag="PKH54_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC99975.1"
                     /translation="MSRSEIRVGGLGGQGVILCGTIIGKAASIYDHKHSGMIQSFGPE
                     ARGSSCSAQVILSDEVIGYPYVRNLDVLVAMSNDACHKFLPELKKDGVLLYEEELVSF
                     PEGTRPAKAWGIPATRFAEELGRKLVLNIVMVGFFAGVTGLVSRQAAEAAVRDSVPAG
                     TEGLNLKAFARGYEYGRELVGGAA"
     gene            complement(3720..>4529)
                     /locus_tag="PKH54_08520"
     CDS             complement(3720..>4529)
                     /locus_tag="PKH54_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOC99976.1"
                     /translation="HIWCPGCGIGTVVNCFSRALMSSGHDMNNVAVVSGIGCTGRVAG
                     YMNLDSFHTTHGRSIPFATGLRLANPGLKVVVFSGDGDLFAIGGNHFLHAARRNLDML
                     IVCVNNFTYGMTGGQVAPTTPLKSTQSTMPFGNFEPPMNLPFVADAAGASYVARWTVF
                     HIRQLVRAMKEGMEHKGFSFIEIMSPCPTLYLRRNKLGDGVDQVRYFKEHSVLKNGAN
                     TQDVAITATDPIVVGTFVERERPTWLDSRNAQLTATLGDRFVPYGGQNVQK"
BASE COUNT          947 a         1419 c         1218 g          945 t
ORIGIN      
        1 gcctcggaaa ccggccgaca caacggtcgt ccccattccc gcaagctccc ggttcatcat
       61 ggccgccttg acaatccggc ggttggcgta ctcgatcgcg gcacagatgc tgcgttccca
      121 taccgctcga tcgtcaaaag gcttgtccag caggcccttg aaaaaggcta tcgtcttgcc
      181 ggtgccggcg ccggtcagga attcacgaac ggaatcgatg cagagctggg acgccaggtc
      241 accgcgcttg tgcccgccca tgccgtctgc gaccagatag aacgaccttt ccggaagaat
      301 caggtgtgcg tcctcgttcc gcttcctgac cttgccgaca gtcgtctcgg cccacgcgga
      361 tatgcggcac tccttcgcca tcaattcacc tggctgcggc cgggacgtcg ggaggcggaa
      421 gaatcagcgg cctctcgttc gtgtcctcgg ttctttgcag ttccatttcg gaaaagagta
      481 cctccggggc caccgtcgat accggaaccg aaccggattc agcgccgcaa tacccgttga
      541 aaacaccgta cctgtcggag gtcgcgataa tgcggtttat cgcggacagc ggtgttccga
      601 ctatctcgac gccacgaacc atcgtcttct ttccggttgc cgcatcaacc ttgtacgcga
      661 tcagcggctt gcccttgaaa gcctggtagc cataggaact tgtgttcgta tttccacctg
      721 caatcgtttc aacgtaaagt ccccaaggct tgttctgctt cttcatttcc tgcaggaaac
      781 gggaaaacag cctggcctgc gacatcggcc tggcgccctg aatgaacaga ttcgccatcc
      841 tggcctgggc cggacggaaa ccctgcgtac gaccgtggcc gttcgacttc tcgaacccct
      901 cgattggctt gcgcgacatc aaaaaggcct tcagaacgcc cttttccacc aggctgacct
      961 tctggccccg cacaccttca tcatcgacac gataaaaacc gttcagcgaa acgcgaccgc
     1021 tgcccgttcc aaacatctcg accgagggat catcgaagat atccaggaat gcgggaagaa
     1081 tcgcctggtt caggtaactc ttgaacgtgc ggccctcttc ctcgtcctcc tggcgctgtc
     1141 cctccagcct gtggccgatg gtctcgtgaa agaatacccc ggtcgcctcg ggcgacatca
     1201 ggacgggaac ggtcgccgga tcaagcacgg gggcatcctt catcgccaca aggttccggg
     1261 caagagtccg tatgcggtcg gctgcggtcg caagatccgg catgtccccc ggcctgcgtc
     1321 cgtatatcgt cacgccgtcc tgaagcagca tgccgtccgg agccctgacg gtagcctggg
     1381 caagcagttg gtagtaatga tcaacggttc ttatcctggt tccctcactg ttgacaatgt
     1441 acctgacgac cctgaaggcc gatatctcga caccggaatc gaagatgtcg ggatactgca
     1501 gaaaaattgc agaaagctcc cttgcatgac cttcacgtgc cggccggtcg aattcggctt
     1561 ccgatatctc ctcaatcaac tccacgggct gttccctgga aaggctggct gacttcctgg
     1621 gttcgctgga gttgaccagc ttcgccttga cgttgtggaa ggacatcagc gccgccttgt
     1681 atgcatgatc cgacagaagc cacaaagttc ggcgcagggc cggtatcgaa tcatcgattg
     1741 ggcccatgaa tccaggattg tacttctggt tgaaattgaa acctgcgtcc ttgtcctcgg
     1801 acgaatccat gtcataactg ccgacccgga tgtcgacgtc ggccatcctt gtgcggccgt
     1861 cgttcgagga ctgaagcgcg ccgttcctgg cgctgacgaa tgacgaccgg acatccctga
     1921 cggcgtaata gacgaaatac ggaaggtcgt aatccccggc ttcaagtcga agcagattca
     1981 cgctgcgatc cagttcctgg agcatggcct ccatcaccgg gtcggcctgc tgctccgaag
     2041 aacgggccgg catcgcgaca agaaccagca gcattactgc cggaaagatc gatttactga
     2101 ccatcttaac gccttcacag cgccgacgat tcgccggcga tcaggccgac cgcctcgaca
     2161 tcggcgggcg ggaacctgaa actcttcaat tcgcccggtt ccacccaact gaagccggaa
     2221 atcccgatta aagcgatttc cccgcccagg atcacacaat tgtacagatg aaaatccaga
     2281 atgaatcgtt catattcgaa caaatttgaa tggaatatgc cgcctacatc tacctgaagg
     2341 cccatttctt ctgaaatttc gcgacggagg gcttctccgt cggactcgcc cggctcgacc
     2401 tttccaccgg gaaactccca ccacccgcca aattctgaat tggccggcct ctgggtaatc
     2461 agtactttcg tgccgtcacg gacaagggcc gcgacaaccc ggaccaatct cttctgctgc
     2521 aatctaaggc tgcccccggg ccggctagta ttcgcgcatc ggactgtaat cagccacaag
     2581 cgcttccatc ttgtcccaga tatgtgaaac gtcgtatgaa taaaccgcca ggtcgtgctt
     2641 cctgccggca agatccttga cgtgaagctt gagcgttgct tccttgcgga acctcatctt
     2701 ttcaagggcc agcatcgccg ccttgttttc cgtcgatgtt tctgccagca ccttctcgat
     2761 gcccgcgttg acgccatccg caatcaggag ccttgaaaga atcgttccaa gtccctggtg
     2821 ctgcaattcc ctggcgatta tcacgcgaag ctggccgatg tgggtcattg acccgaagtg
     2881 gttgcgatcg atttcggccg aggcgacgat ccgaccgtca agttccgcca caacggccct
     2941 gacccggtcg tgcgtatcgt caatcatgaa gttgtgaacc cggatcgggt cggtcacgtc
     3001 gttccgcagg aagtgcctgt cctcaagcgg aagaagacga aagaactcga ataccgcggg
     3061 ctcgtcggcg cgaaccatgt cgcgcacaag gacttcgaca ccgttcttaa gagcgaattt
     3121 tgcaggaagt ttcaccgatt ccatgctttc tcccctgccg gcagacaagg ccggcccgtc
     3181 aggccgcgcc gccgaccagt tcacgcccgt attcgtaacc gcgcgcgaac gccttcaggt
     3241 taaggccttc ggttccggct ggcaccgaat ccctgacggc ggcctcggcc gcctgacggg
     3301 aaaccaggcc agtcacaccc gcgaaaaacc cgaccatcac gatgttcaga accagcttgc
     3361 ggccaagctc ttccgcgaac ctggtcgccg ggattcccca agccttcgcc ggcctggttc
     3421 cctcgggaaa cgaaaccagc tcttcctcgt atagcaggac tccgtccttc ttcagttccg
     3481 gaagaaactt gtggcacgca tcgttcgaca tcgcgaccag gacgtcaagg ttcctgacgt
     3541 agggatatcc gatgacttcg tccgacagga tgacctgggc gctgcacgaa ctgccgcggg
     3601 cctccggccc gaacgactgg atcatgcccg aatgcttgtg atcgtatatc gaggcggcct
     3661 ttccgattat ggtgccgcaa aggattacgc cctggccgcc aaggcccccg acgcgtattt
     3721 cacttctgga cattctggcc tccgtaagga acgaacctgt caccaagggt tgccgtaagc
     3781 tgtgcattcc ttgagtcgag ccaggtcggc ctttcgcgtt caacgaaggt tccaaccacg
     3841 atcggatcgg tcgccgtgat ggcgacatcc tgcgtgttgg cgccgttctt caagacgctg
     3901 tgctccttga agtaccgaac ctggtcaacg ccgtcgccaa gcttgttgcg acgcaggtaa
     3961 agggtcggac acggactcat gatctcgatg aaggaaaatc ctttgtgttc catgccttcc
     4021 ttcatcgccc tgacaagctg cctgatgtgg aataccgtcc agcgggcgac atagctcgcc
     4081 ccggccgcat cggcaacgaa aggaaggttc atcggcggtt cgaagttgcc gaaaggcatc
     4141 gtgctctggg tcgatttcag tggagtcgtc ggggcgacct gtcccccggt catgccgtat
     4201 gtgaaattgt tcacgcagac aatcagcatg tccaggttgc gccgcgccgc gtgcaggaaa
     4261 tgatttccac cgatcgcgaa aagatcgccg tcaccggaaa acacgacaac cttcagtccg
     4321 ggattggcaa gacgcagccc ggtcgcgaac ggaattgaac gaccgtgggt cgtgtggaac
     4381 gagtccaggt tcatgtatcc cgccacgcgg ccggtacaac cgattcccga taccaccgcc
     4441 acgttgttca tgtcatgacc ggaagacatc agcgcgcgcg aaaagcagtt gacgacggtt
     4501 ccgatcccgc agcccggaca ccagatatg
//
LOCUS       DAOPPA010000254         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20810_length_4526_cov_3.078953, whole genome shotgun sequence.
ACCESSION   DAOPPA010000254 DAOPPA010000000
VERSION     DAOPPA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20810_length_4526_cov_3.078953"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKH54_08525"
     CDS             complement(<1..312)
                     /locus_tag="PKH54_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99977.1"
                     /translation="MNDGKSQEAARLASALIAVLSSTQDLIPDLPVAGGSRRARVART
                     PGIAIQDEPAQAAPTPPRAGQTPAVQDADQFLKSLPDALGPLREAVAGCGRCRLASGR
                     RH"
     gene            complement(317..1546)
                     /gene="coaBC"
                     /locus_tag="PKH54_08530"
     CDS             complement(317..1546)
                     /gene="coaBC"
                     /locus_tag="PKH54_08530"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOC99978.1"
                     /translation="MISMKGKQVLLGVTGGIAAYKAAFLARELMRAGAAVTTVLTESA
                     GKFITPLTFESLTERPCRTDDDMFGGLSGETHISLARDKDLLVIAPATATTIASLALG
                     TASNLLTATVLASRCPIVLCPAMHTQMWESVPVRRNIDLLMQDPRYVVVPPDEGDLAS
                     GDHGPGRLPDASRIVAHCLAALTPRTLAGKTVVVTAGPTREQIDPVRCITNPSSGRMG
                     FFLAESARRRGADVRLILGPCDISPDFGGPGGTFTVTRVQTTTEMLEAVREAINGADA
                     LLMSAAPADERSVRRASGKIRKEDFETSIEIEPNPDILATLSASTAGMVVLAFAAETS
                     VSEDMARAKMKRKGADLLFANPAGDGKGFGDVRNGGTLVAADLAEVLTIEDESKESLA
                     EILVDAVVDAMNRKAGR"
     gene            complement(1604..2824)
                     /locus_tag="PKH54_08535"
     CDS             complement(1604..2824)
                     /locus_tag="PKH54_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC99979.1"
                     /translation="MKGIPSSGVVSFRLFLAGSLAACLLVASCVPQSQIRKRARDVSS
                     LIGDQRESIYNCAPVELATAEINIEIARNESSLGRSVAAVRHMKLAEEAAEKAFAQSR
                     DNVCLNDSDGDKVPDRKDRCPNQPEDVDGFQDADGCPDPDNDGDTVLDDQDECRDVPG
                     PVMNKGCPIVDTDGDGLPDKEDRCPQVKGLIANFGCPIVDTDGDGLPDSEDRCPYETG
                     LIANYGCPIADRDQDKVPDVEDACPDEPGPISTRGCPYRDTDGDGLLDPDDLCPMDPG
                     PKDHKGCPYKRIEITEKKIELREKIFFVSGKSVIKRESWPLLNEIAKAMADHPAIKVD
                     IEGHTDDVGNDRANMKLSQARANSVRAYLVKKGVEPMRMRAVGYGEDRPIDDNSTPEG
                     QAINRRVEFVITAH"
     gene            complement(2821..3144)
                     /locus_tag="PKH54_08540"
     CDS             complement(2821..3144)
                     /locus_tag="PKH54_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOC99980.1"
                     /translation="MKSALWFLILLLPMALACSPISASKAISRADESLKAAQAIGGEA
                     KCPYEFKAATLALEYAKDREGTSDFEAAIEFAKKARELAEEASRKASLIEDGNQARLD
                     QGVTQ"
     gene            complement(3313..>4526)
                     /locus_tag="PKH54_08545"
     CDS             complement(3313..>4526)
                     /locus_tag="PKH54_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99981.1"
                     /translation="YWAVNFKVFNTNGFWEGKFGKPWEKDLNGGLSGALAAHLSFNVF
                     AGTFGRPIPAAYGFNYLRNRYEPRDSVNASSYTNQIYLLEENGARPLYASWDYNYYDP
                     VVVAAGTIYDRLAAFENIADPSSYILAADNQADTQKYYINYGSIFRDEVNALFGGIMA
                     NNADNYGWCIMLHPVSNQPIGFGPRDFVSLEGTNIGCEATYKGCFTKSGTGKADRLVA
                     IHRADIEGNPCSSDQVFVDVMGSALEPEEQYVFPTTQFRIPMLAAYYGYSMLIDNQDR
                     SFIDGTRVWLKGDAYELQPPPGAETALCEDRFTGRQYMAYRIPGDGYWPGFDLVSQCQ
                     EIFKCYDPVLNDTLTQDEAERCEFIAKTKDVSNLTIDDLRDTYLFHQIQFLVGKLELI
                     RTMYTAYEFSY"
BASE COUNT          838 a         1366 c         1364 g          958 t
ORIGIN      
        1 gtgccttcgc cccgaggcca gacggcatcg cccgcatcct gcaaccgcct cgcgcagcgg
       61 accgagggca tccggcaggg acttcaggaa ctggtcggcg tcctgaaccg cgggggtctg
      121 accggccctg ggtggcgtcg gggcagcctg cgcgggttca tcctggatag cgatgccagg
      181 cgttctggcg acgcgcgccc gacgcgaacc tccggccacc ggcaggtcgg ggatcaaatc
      241 ctgtgtcgag gaaaggacgg caatcagcgc ggacgccagg cgcgcggctt cctgggattt
      301 tccatcgttc atagagtcag cgtcccgcct tcctgttcat ggcatcgaca acggcgtcaa
      361 ccaggatctc cgccagcgat tccttcgatt catcctcaat agtcagcact tcggcgagat
      421 ccgcggcaac cagagtcccg ccgttgcgaa cgtccccgaa tcccttgccg tcaccggctg
      481 gattggcgaa caggagatcc gcgccttttc gtttcatctt ggcccttgcc atgtcctcgg
      541 aaacgctggt ttcggccgcg aatgcaagga cgaccattcc ggcggtcgat gcagagagcg
      601 tggcaaggat atccgggttg ggttcgattt cgatgctggt ctcgaaatct tccttgcgaa
      661 tcttgccgga tgcacgacgc accgagcgct cgtcagccgg tgccgcgctc atcagaaggg
      721 cgtcagcgcc gttgatggct tcccgaaccg cctcgagcat ttcggtcgtt gtctgtaccc
      781 tggtgacggt gaaagtccct ccgggacccc cgaaatcggg cgaaatgtcg catggcccca
      841 ggatcaggcg gacatccgcg ccgcgtctgc gggccgactc tgcaagaaag aagcccattc
      901 gtcccgagga cgggttcgtg atgcacctga ccgggtcaat ctgctcccgc gtcgggccgg
      961 cggtcacaac cacggtttta ccggccaggg ttcgcggcgt cagcgctgca aggcagtggg
     1021 cgacaatcct ggaagcgtcc gggagccttc ccgggccatg atcgccgctg gcaaggtcgc
     1081 cctcgtcagg cggaaccacc acgtatcgag gatcctgcat cagcaggtca atgttgcgcc
     1141 gcaccggcac cgactcccac atctgggtgt gcattgccgg gcaaaggact attgggcacc
     1201 ttgaggcaag aaccgtcgcc gtcagaaggt tggatgccgt tcccagtgcc agtgaggcta
     1261 tcgtcgtggc ggtggccggc gcgatgacca gaagatcctt gtccctggca agggaaatat
     1321 gggtttcccc ggaaagcccg ccaaacatgt cgtcgtcggt tcggcacggt ctttcggtaa
     1381 gcgattcgaa tgttagcgga gtgatgaatt tcccggcgga ctcagtgaga acggtcgtaa
     1441 cggccgcgcc cgcgcgcatc agctcgcgtg ccaggaaggc cgccttgtat gcagcaatcc
     1501 cgccggttac cccaagcagc acctgctttc ctttcattga aatcatgaat atggtccttg
     1561 agcgcccggg tcagtaccct ggcgaatcag aagcgttcca gcgtcagtgc gccgttatca
     1621 cgaattcaac gcgccggttg attgcctgtc cttccggcgt ggaattgtcg tcaatcgggc
     1681 gatcctcgcc gtatccgacc gcgcgcatgc gcatcggctc gacccccttc ttgacaagat
     1741 aggcccttac cgagttcgcg cgtgcctgcg aaagcttcat gtttgcccga tcgttgccaa
     1801 catcgtccgt gtggccttcg atatcgacct tgatggcagg gtgatcggcc attgccttgg
     1861 caatttcgtt cagcagcggc cacgactcgc gcttgatgac cgactttcct gaaacgaaga
     1921 agatcttctc gcgcaattcg atcttctttt ccgtgatttc gattctcttg taggggcaac
     1981 ccttgtgatc cttcggtccc ggatccatcg ggcagaggtc gtccggatca agcagcccgt
     2041 cgccatcggt gtccctgtaa gggcaacccc ttgtcgaaat cggcccgggc tcgtccgggc
     2101 aggcgtcttc gacatcggga accttgtcct gatcccggtc ggcaatcggg catccgtaat
     2161 tggcgatcaa tcccgtttca tagggacacc gatcctcgct gtcgggcagc ccgtcgccgt
     2221 cggtatcgac aatcggacat ccgaaattgg cgattaaccc cttgacctgc gggcacctgt
     2281 cctccttgtc cggaagtccg tcgccgtccg tatcgacaat tggacatccc ttattcatga
     2341 cggggcccgg gacgtcacgg cattcgtcct gatcgtccaa aaccgtatca ccgtcattat
     2401 ccggatccgg acagccgtcg gcatcctgga acccatcgac gtcctccggc tggttcgggc
     2461 atcgatcctt ccggtcgggc accttgtcgc catcggagtc gttcaggcac acgttgtcgc
     2521 gggactgtgc gaatgccttc tccgcggcct cctcggcgag tttcatgtgg cggacggccg
     2581 cgacggatct gccaagcgag ctttcattcc tggctatctc gatattgatt tccgcggtcg
     2641 cgagttcgac cggagcacag ttgtaaatgg attcccgctg atcgccgatc aggctggaaa
     2701 cgtcccgggc gcgcttgcga atctgggact gcgggacgca cgatgcaacc agcagacagg
     2761 cggccaggga tccggccagg aaaaggcgga aggacacgac gccggaagag ggaatgcctt
     2821 tcattgggta accccctgat ccagtctggc ctggttgccg tcctcgataa gcgaggcctt
     2881 tctcgatgcc tcctcggcaa gttcccgggc cttcttcgcg aactcgatcg ccgcctcaaa
     2941 gtccgatgtt ccctcgcgat cctttgcgta ttcaagtgcc agcgtggcgg ccttgaattc
     3001 gtaaggacac ttggcctcgc cgccaatcgc ctgggccgcc ttgagtgatt cgtcggcgcg
     3061 cgagatggcc ttgcttgcgg aaatggggct gcacgccagg gccatcggaa gcagcagtat
     3121 cagaaaccac aatgcagact tcacattgtc ctccggttcc gatcggatca atccctgccg
     3181 gcaacgccct tccgacttga agaagttgcg gggcagccca ttatcacgct ttttgggcct
     3241 tcagacaaca gtcggtgctt gtgttcaagc tgtctggcga actgaccgat tcagacgtga
     3301 ttggtcgtcg gatcagtagg agaactcgta agcggtgtac atggtgcgga tcagttcgag
     3361 cttgccgacc aggaactgga tctggtggaa caggtaggtg tcgcgcaggt cgtcaatcgt
     3421 aaggttgctg acgtccttgg tcttcgcgat gaactcgcac cgttccgcct cgtcctgggt
     3481 gagcgtgtcg ttaaggacgg ggtcatagca cttgaagatt tcctggcact gagagaccag
     3541 gtcgaatccc ggccagtatc catcgccagg aatgcggtac gccatgtact gccgaccggt
     3601 gaagcgatcc tcgcacaggg cggtctcggc gcccgggggc ggctggagtt cataggcatc
     3661 gcccttcaac cacacgcggg taccatcgat gaacgaccga tcctggttgt cgataagcat
     3721 cgagtaaccg tagtaggccg caagcatcgg gatacggaac tgggtggtcg ggaagacgta
     3781 ctgttcttcg ggctcaaggg ccgaacccat tacatccacg aaaacctgat ccgacgagca
     3841 cggattacct tcgatgtcgg cgcggtggat cgccacgagc ctgtccgcct ttccggtgcc
     3901 ggacttcgtg aagcagccct tgtaggtcgc ttcgcagccg atattggtgc cctcaaggct
     3961 gacgaagtca cgtggaccaa aaccgatcgg ctggttggaa accgggtgca gcatgatgca
     4021 ccagccgtaa ttgtcggcgt tgttggccat gatgcccccg aacagggcgt tgacttcatc
     4081 acggaagatc gacccgtaat tgatgtagta cttctgggtg tcagcctggt tgtccgcagc
     4141 caggatgtac gatgacgggt cggcgatatt ttcgaatgct gccagtcggt catagatggt
     4201 acccgcggca acgactaccg ggtcgtagta gttgtaatcc cacgaggcgt aaagcgggcg
     4261 ggcgccgttt tcttccagca ggtagatctg gttcgtgtag gaactggcgt ttacggagtc
     4321 acgcggttcg tacctgttgc gcaggtaatt gaacccgtac gcggccggga tcggacggcc
     4381 aaaggtgccc gcgaatacgt tgaacgacag gtgggctgcc agtgcgccgg acaggccgcc
     4441 gttaagatcc ttttcccacg gcttgccgaa tttgccttcc cagaagccgt tggtgttgaa
     4501 gaccttgaaa ttgactgccc agtact
//
LOCUS       DAOPPA010000255         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20912_length_4509_cov_4.114504, whole genome shotgun sequence.
ACCESSION   DAOPPA010000255 DAOPPA010000000
VERSION     DAOPPA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20912_length_4509_cov_4.114504"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..215)
                     /locus_tag="PKH54_08550"
     CDS             complement(24..215)
                     /locus_tag="PKH54_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOC99982.1"
                     /translation="MSMKITDDCVCCGSCIDECDINHAISEGDPIYVIDPAKCTECAE
                     AGGEPKCAPVCATEAIVKA"
     gene            complement(379..876)
                     /locus_tag="PKH54_08555"
     CDS             complement(379..876)
                     /locus_tag="PKH54_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99983.1"
                     /translation="MERDMHGETSDVGHIGETVADGGERRDGMLSELARKAVASSVSK
                     LVETEGFVREIVKAMSREVAAYIGRELASVRQEVVDQATDKIVGWLDKVDIAGEIRKS
                     LDGMTFDINVKVHVTDEGLKTGPAPVRKPSAAGKPSNARKSSGALKSAHAGKPVAARK
                     VATRK"
     gene            1232..4099
                     /gene="secA"
                     /locus_tag="PKH54_08560"
     CDS             1232..4099
                     /gene="secA"
                     /locus_tag="PKH54_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOC99984.1"
                     /translation="MKAFFRKLFGTKNERELKRMRPIIERINSLEPEFQKLTDDDLRG
                     LTAKFRKRLTEGETLDDILPEAFAAVREASVRSTGMRHYDVQMIGGIALHRGTISEMK
                     TGEGKTLVATSALFLNALEPDPKNPERGMGAHLVTVNDYLAKRDSEWMGRIYNALGMS
                     VGVIVHGLTDRERQAAYACDITYGTNNEFGFDYLRDNMKFNLGDMVHRYYDFPKKGEQ
                     VKTLHYAIVDEVDSILIDEARTPLIISGPAEESSEVYYTVNAIVPMLRRDEDYIVDEK
                     ASAVTLTESGVDKIERRLQVKNLYDGDNVELVHHVYQALKAHTLFKRDVNYVIEDNKI
                     VIVDEFTGRKMPGRRWSDGLHQAVEAKEGLKIENENQTLATITFQNLFRMYNKLAGMT
                     GTADTEAEEFARIYDLDVLVIPTNRPIGRKDSEDLVYKNDRAKFMAVLDQIKECYGRG
                     QPVLVGTTSVEKSELVSRLLKNSKIPHHVLNAKNHMLEAQIIAQAGRKFAVTISTNMA
                     GRGTDIVLGGNPEALARTQVDSIESDKFQELVQEYKPICANERQEVLAAGGLFIIGTE
                     RHESRRVDNQLRGRAGRQGDPGASQFFLSLDDDLMRIFGGDQIKNIMDRLNVPDNEPI
                     VHKWVTRAIESAQKRVEGRNFDIRKNLLEYDDVMNQQRQTIYRLRNRVLEGSEVHSMI
                     LDAVEQTAWNLFNQFCPEGVNPEEWDFESLAREVLGSLRVKVSEEDLAAEGERAGDLL
                     VKLLREAYDRKTELSVKKALDIRLPEPEFVTPDFDREAWLADRAELERRMIEGFRLYE
                     RERYLHAIDSHWKNHLYAMDHLKEGVHLEAYAQKDPKVIYKKEGFELFKQLLDRIDQS
                     IAETMFRVDISGPTDVESIRRLRRSVAMHYGRGALPPEATGVNSSQRTNQMDEAGPVA
                     SSPNAGFVPRAGRNDPCPCGSGKKYKKCCLPKEMGVDPASF"
     gene            4249..>4509
                     /locus_tag="PKH54_08565"
     CDS             4249..>4509
                     /locus_tag="PKH54_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOC99985.1"
                     /translation="MYETSDIRKGLKITMDGYPWEVVDFQFVKPGKGTAFTRVRVKNL
                     KTGQALDRTFKTGEKLEECDLEEHVGQYMYSDGENYFFMNQES"
BASE COUNT         1029 a         1331 c         1310 g          839 t
ORIGIN      
        1 ccctgttcga tcgcggtctg atatcaggcc ttgacgatgg cttcggtcgc gcagaccggg
       61 gcgcacttcg gctcgccacc ggcctcggca cattccgtgc acttcgcggg gtcgatcacg
      121 taaatgggat cgccttcgga aatggcatgg ttgatgtcgc actcgtcaat gcacgagccg
      181 cagcacacgc agtcatcagt gatcttcatg gacatgggaa acctcccgga ttcaaaaaac
      241 ttgtcaaatc gggaaggctg tccttcccac gggccgcatc atcgataaaa gtggcctgtc
      301 tgtcaaggtt ccacgacacg gattgacagt cgaccttcgc ttgaccagcg gaaggctgct
      361 tcaggtttcg gcctgccctc acttcctcgt ggcgaccttg cgggcggcga ccggctttcc
      421 ggcgtgggcc gacttcaagg cgccggagga cttccgggcg ttcgaaggct ttcctgcggc
      481 ggaaggcttg cgaacggggg ccgggccggt tttaagaccc tcgtccgtga cgtgcacttt
      541 gacgttgatg tcgaaagtca ttccgtccag ggacttgcga atctcaccgg cgatatcgac
      601 cttgtccagc cagccgacaa tcttgtcggt ggcctgatcg acgacttcct gccgcacgct
      661 tgccagctcg cggccgatgt atgcggcgac ctcccggctc atggccttca cgatctcgcg
      721 gacaaagcct tccgtttcga ccaactttga aaccgacgac gccacggcct ttcgggccag
      781 ttcggacagc atgccatccc tgcgttcccc gccatcggca accgtctcgc caatgtgacc
      841 gacatcggaa gtttcaccgt gcatgtcccg ttccataagc cccccgccat agcgcgtgga
      901 tacgtgcgcc catgccacaa caaaatcaca ggcgcgtcgt cttatcgacc gatggattct
      961 agcaccaacg ggcgcgacgc gaaatcccga cactgccggg ggttgcgcat gcgccgttcg
     1021 ttgccacatt acagggcgcg gcggcagttg atctgtgcta aaatccgcgc gccattttcc
     1081 ggcgccggcg atcctggctc tgcgccgacg gccccgtgcg ggcgaatgaa aattcgcccg
     1141 tgatttgaaa acattgccgg cccgtatggg cgaatgaaaa ttcgcccgtc gctgcctgcg
     1201 cgtaataatt agcggttcga tgggagaccc gatgaaggct ttttttcgaa aacttttcgg
     1261 tacgaagaac gaacgtgaac tgaagcggat gcggccgata atcgagcgca tcaacagcct
     1321 ggaaccggaa ttccagaagc tgaccgacga cgacctgcgt gggctgacgg ctaagttccg
     1381 caagcggctg acagaaggcg agacgcttga cgacatcctt ccggaagcgt tcgcggcggt
     1441 tcgcgaggcg tcggtccgtt cgaccggcat gcgccattac gacgttcaga tgatcggcgg
     1501 tatcgcactg catcgcggaa cgatttccga aatgaaaacc ggcgaaggca agaccctggt
     1561 cgccacatcc gccctgttcc tgaacgcgct tgaaccggat ccgaaaaacc ccgagcgcgg
     1621 gatgggcgcc caccttgtaa ccgtcaacga ttacctggca aagcgcgact ccgaatggat
     1681 ggggcgcatc tacaacgcac tgggcatgtc ggtgggcgta atcgtccacg ggctgaccga
     1741 tcgcgaacgt caggctgcct atgcctgtga cataacttac ggaacaaaca acgaattcgg
     1801 tttcgattac ctgcgcgaca atatgaagtt caacctgggg gacatggtcc acaggtacta
     1861 cgacttcccg aagaaaggcg agcaggtcaa gacactgcat tacgccatcg ttgacgaagt
     1921 cgactcgatt cttatcgacg aagcgcgcac gccactgatc atttccggtc ctgccgagga
     1981 atcttccgaa gtttattaca ccgtgaacgc catcgtcccg atgctgcgcc gcgatgaaga
     2041 ctacatcgtc gatgaaaagg cctcggcggt gactctgacc gaatcgggcg tcgacaagat
     2101 tgaaaggcgg cttcaggtga agaacctgta cgacggcgac aacgtcgaac tggtccacca
     2161 cgtctatcag gcattgaagg cccacacgct gttcaagcgc gatgtcaatt acgtcatcga
     2221 ggacaacaag attgtcatcg ttgacgagtt cactggtcga aagatgccgg gtcggcgctg
     2281 gagcgacggt ctgcaccagg cggtcgaggc caaggaaggc ctgaagatcg aaaacgaaaa
     2341 ccagacgctg gcgacgatca ctttccagaa cctgttccgc atgtacaaca aactggccgg
     2401 catgaccggt accgccgata cggaagccga ggaattcgcg cgcatttacg acctggacgt
     2461 actggtcatt ccgaccaaca ggccgattgg caggaaggac agcgaggatc ttgtttacaa
     2521 gaacgaccgc gcgaagttca tggcggttct ggatcagatc aaggaatgtt acggtcgcgg
     2581 ccagccggtt ctggtcggca cgacatccgt cgagaagtcc gaacttgtca gccggcttct
     2641 gaagaacagc aagattccgc accacgtcct gaatgccaag aaccacatgc tggaagcgca
     2701 gattatcgct caggcaggcc gcaagttcgc ggtcacgatt tcgaccaaca tggccggccg
     2761 cggtaccgac atcgtccttg gcggcaatcc ggaagcgctt gcccgtaccc aggtcgattc
     2821 gattgaaagc gacaaattcc aggaactggt gcaggagtac aagccaatct gcgcgaacga
     2881 gcgccaggaa gtcctggccg ccggcggtct tttcatcatc ggtaccgaac gtcacgaatc
     2941 ccggcgagtc gataaccagt tgcgcggccg cgccggacgt cagggcgacc cgggtgcgtc
     3001 gcagttcttc ctgtcgctgg acgacgatct gatgcgcatc ttcggcggcg accagatcaa
     3061 gaacatcatg gatcgcctga atgtcccgga caacgaacca atcgttcaca aatgggtcac
     3121 aagggcaatc gaaagcgcgc agaaacgggt tgagggacga aacttcgaca tccgcaagaa
     3181 cctgctggaa tacgacgacg tcatgaacca gcagcgccag accatttacc gtttgcgcaa
     3241 cagggttctg gaaggcagtg aagtccattc gatgatcctg gacgccgtcg agcagaccgc
     3301 gtggaacctt ttcaaccagt tctgcccgga aggcgtcaat cccgaagaat gggacttcga
     3361 aagcctggcc cgggaagttc ttggaagcct gcgtgtcaag gtcagcgaag aagatctggc
     3421 ggccgaaggt gaacgtgccg gcgacctgct ggtcaagctt ctgcgcgagg cgtacgacag
     3481 gaagaccgaa ctttccgtca agaaggcgct tgacatcagg ctgcccgagc cggaattcgt
     3541 cacgccggac ttcgatcggg aggcctggct ggccgaccgc gccgaactgg aacgccgcat
     3601 gatcgaagga ttccgtctgt acgaacgcga acgttacctt catgccatcg acagccactg
     3661 gaaaaaccac ctgtacgcga tggaccacct gaaggaaggc gtgcacctgg aggcttacgc
     3721 ccagaaggac ccaaaggtca tctataagaa agagggtttc gagctgttca agcaattgct
     3781 ggatcgcatc gaccagtcga tcgccgaaac gatgttccgg gtcgatatca gcggtccgac
     3841 cgatgttgaa tccattcggc gcctgcgccg cagcgtcgcc atgcattacg gacgcggcgc
     3901 gctgccgccc gaggcgaccg gggtcaattc cagccagaga acgaaccaga tggacgaggc
     3961 cggaccggtc gcgtcttcac cgaatgccgg tttcgtcccg agggccggcc gcaacgaccc
     4021 ctgcccctgc ggcagcggca aaaaatacaa gaagtgctgc ctgcccaagg aaatgggcgt
     4081 tgatcccgca agcttctgac gcgcgcatcc cccggcggaa aaggggcctg ccccgagcga
     4141 tccgtggtca gccttttgaa ccagtgaaac gagtcttggg gttgcgcaag tccaaaattt
     4201 aatgtataaa tcgcccgctt tttccgaact caaacgatcg aggcgagcat gtacgagact
     4261 tccgatatcc gtaagggact caaaatcacg atggacggct acccctggga agtcgtcgat
     4321 ttccagttcg tcaagccggg caagggcacc gcgttcaccc gtgtgcgcgt caagaacctg
     4381 aagaccggcc aggcgctgga caggaccttc aagaccggtg aaaagctgga agagtgcgac
     4441 cttgaagaac acgtcggcca gtacatgtac agcgacggtg aaaactactt cttcatgaac
     4501 caggaatcg
//
LOCUS       DAOPPA010000256         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_20998_length_4494_cov_3.267403, whole genome shotgun sequence.
ACCESSION   DAOPPA010000256 DAOPPA010000000
VERSION     DAOPPA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20998_length_4494_cov_3.267403"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKH54_08570"
     CDS             <1..370
                     /locus_tag="PKH54_08570"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="functions in pyrimidine salvage; pyrimidine
                     ribonucleoside kinase; phosphorylates nucleosides or
                     dinucleosides to make UMP or CMP using ATP or GTP as the
                     donor; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOC99986.1"
                     /translation="TRKTETLRVEPKDTIIVEGILVLEMDVVRREMDLKIYVDTDSDL
                     RFIRRLRRDMQERARSLDSVIEQYLTTVRPMHMEFVERSRQRADIVVPWMNFNTVAVD
                     MVISQVRNGRPRSLRDRIEP"
     gene            427..2115
                     /locus_tag="PKH54_08575"
     CDS             427..2115
                     /locus_tag="PKH54_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOC99987.1"
                     /translation="MADRTISRLVVVVAAAFLAGAFVTAQPVPAFSQTAKATTKKTVG
                     KATTKKTVGKATTKKTTGKATKTSEKSSKKSGKTAKNESCGKPTRHKVADGDSLWKIA
                     GKYGTTVDALKRLNGKQASNLHPGRILIVKAAKPCAKVKETRSATPSNAAASPSASLE
                     DGPSETGVVFTGEPVDGHRLSESASTPGTEASASGPRADDVQPAQSADFPDPSAIRQE
                     LARAGAYDGTPEIGHDATPPDLVGVPPAQPCELDGFELGAAGDAEDDDDESGIAEDYE
                     TGEMPEIELNEFGFSDDGAACAEYLGQFTKMPENPKGSVNIYHTVRKGDTLGRIAKKY
                     RTTVKNLQSMNGLRNNPKKARRLMHPGKKVLIRIGDPKDLVAHRPFLKDWVKLTDGEG
                     FSIKRPSTAYGRPWAMNLMLRAFCELRRRFPDTDNIVVGDWSGIVAGQLSRHLSHKTG
                     RDVDLSYFARGRTGLKHFIKVNAKTMDVGPSWELVRALLDTRQVEYIFMDWTLQREMY
                     EYALERGFKKAELDRVFQYPEGRRVRTGIIRWSKGHDDHIHVRFRCPKDDNLCR"
     gene            2305..3513
                     /locus_tag="PKH54_08580"
     CDS             2305..3513
                     /locus_tag="PKH54_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOC99988.1"
                     /translation="MSKIRISAHRIGKISTILSMLFLSCLAIACDEGRGIFDQCGGKW
                     PDGNCYAQVRDPAGDNIELATAIMDRYIQEYPADTLGWSWEPTVLMTGVYELYRVTGH
                     ERFLHYMRDWMDANLEIGYFMFCSDFASPALIAAFLYMETGEERYLAVVQDAFTYLEE
                     TVIRTDDGGFNHWGEAEFNGGATLWLDTLFMVGTMLTRWGEFVNDSGALDEMGLQIAV
                     FTARLQSEGGLYVHAFNYNKPVDTDIYWGRGNGWVSAAGFDYLRARLVRGERDVVAEE
                     ALVAQAQAARFAQDQESGLWWIVLNRPGEAYVETSTAALFAYGMARGWRYGLLFDEFL
                     PVIKRAMDGIRATITTDDLGRPVVSNVSGPTEAGTFEYYAGLPVADDLGYGVGAVVLA
                     LVETSGLPIE"
     gene            complement(3521..4345)
                     /locus_tag="PKH54_08585"
     CDS             complement(3521..4345)
                     /locus_tag="PKH54_08585"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOC99989.1"
                     /translation="MQSWQAAVLGVVEGITEYLPVSSTGHLLLAERLMNIPRTSAADS
                     FAIAIQLGAILAILAVYGSRVSQMAKGLVGRDDEGRNLALNVLVAFVPAAVAGFLAGD
                     FIKTYLFNLNIVAGAWALWGLAILVIFMNPRFCDPSIGQPLETLTWRGALTIGLFQLL
                     ALVPGTSRSLVTIIGALVVGLSLPSAVEFSFLLGVVTLGAATVWDLTSHFGELTAEFG
                     WEPLLIGMIVSFVSALGAVKWMVYYLSRHGMRIFGWYRLGIAALTIALVAAGFIGK"
BASE COUNT          981 a         1243 c         1396 g          874 t
ORIGIN      
        1 tacgcgaaag accgagactc tgcgcgtgga accgaaggac accatcatcg tcgaaggtat
       61 ccttgttctg gaaatggatg tcgtgcggcg tgaaatggat ctgaagatct atgtcgatac
      121 ggacagcgat ctgcgattca ttcgcaggtt gcgtcgcgac atgcaggaac gtgcccgaag
      181 cctggattca gtgattgaac agtaccttac gacagttcga ccgatgcaca tggaattcgt
      241 cgagcgaagc cgacagaggg ccgacatcgt ggtgccatgg atgaatttca atacggtggc
      301 cgttgatatg gtcatcagtc aggtccggaa cggccgtccc aggtcgcttc gcgatcgaat
      361 cgaaccgtag gcggatctga cggccagccg gccggagttc cggcaacagt tgatggtggg
      421 ggcctgatgg cggacagaac tatttcccgt ctggtcgtgg tcgtggcggc cgcgtttctg
      481 gccggcgcct ttgtaacggc acaacctgtc ccggcatttt cacagaccgc caaggcgacg
      541 actaagaaga cggtcggcaa ggcgacgact aagaagacgg tcggcaaggc gacgactaag
      601 aagacgaccg gcaaggcgac gaagacttca gaaaaatcct caaagaaatc cggcaagact
      661 gcgaagaacg aatcctgcgg gaagccgacc aggcacaagg tcgccgatgg cgacagcctc
      721 tggaagatcg ctggaaaata cgggactacg gttgacgcgc tgaagcggct taacggaaag
      781 caggccagca atctgcatcc tggccggatc ttgattgtca aagccgcaaa gccgtgcgcg
      841 aaggtcaagg aaacccgttc ggccacacct tcaaatgccg cggcttcgcc ctcggcgagt
      901 ctggaggacg gcccttccga aacgggtgtc gtcttcacag gcgaacctgt tgacggccac
      961 cgcctttccg aatccgcttc cacgcccggc accgaagctt ctgcatccgg tccgcgggcg
     1021 gatgacgtcc aaccggcgca gtcggcggat tttccggatc cgtccgcgat ccgtcaggaa
     1081 ttggccaggg cgggcgcgta tgatggcacg cccgagatcg ggcacgacgc gactcccccc
     1141 gatctcgtcg gagtaccgcc ggcccagcca tgcgaactgg acggattcga actcggcgcg
     1201 gcgggcgatg cggaagacga cgacgacgag tccgggattg cggaagacta cgagaccggc
     1261 gagatgcctg aaatcgagct caatgaattc ggtttttcag acgacggcgc cgcttgtgcc
     1321 gaataccttg gccagttcac gaaaatgcct gaaaacccga agggttccgt gaatatctat
     1381 cacacggtca ggaagggcga cacccttggc aggattgcga agaagtaccg tacgacagtc
     1441 aagaatcttc agtcgatgaa cgggttgagg aacaatccaa agaaggcaag gcggctgatg
     1501 catccgggaa agaaggttct gattcgaatc ggggatccaa aggatcttgt tgcgcatcga
     1561 ccgttcctga aggactgggt gaagctgacc gacggtgaag gtttctcgat caagcgccca
     1621 tcgacggcat atggacgtcc gtgggccatg aacctgatgc tgcgggcgtt ctgcgaattg
     1681 cgccggcgtt ttccggacac cgacaatatc gtggtcggtg actggtccgg catcgttgcc
     1741 ggccagcttt cccggcatct ttcgcacaag accggcaggg atgtcgatct ttcgtatttt
     1801 gcccgagggc gaacgggact taagcacttc atcaaggtga atgcgaagac gatggatgtc
     1861 gggccaagct gggaacttgt gcgggccctg ctggacacgc gccaggtcga atacatcttc
     1921 atggactgga cgcttcagcg cgagatgtat gaatacgcgc ttgagcgggg cttcaagaag
     1981 gccgagcttg atcgcgtgtt ccaatatccc gagggacgtc gggttcgaac cggcatcata
     2041 cgctggtcaa aaggccacga cgaccatatc catgtcaggt tcagatgccc gaaggacgac
     2101 aatctctgcc gctgatccgc cttcgcttcg cgttgcggag gttcgggctg acggagtccg
     2161 ttgcacgctg gggcgtacgc cgggatgcgg cgcgatttaa ggcaatgcgc ttgaacgcgg
     2221 cgggcggatg taatgcgccc gtacgttcac ggacgcacta tcgtttgttc tggaattcca
     2281 accggaacct ggaggccgac atcaatgtca aagatacgga tcagcgccca tcgtatcgga
     2341 aaaatttcca caatcctgtc gatgcttttt ctgtcgtgtc tggcgatcgc ctgtgacgaa
     2401 ggtcgtggaa tcttcgacca gtgcggcggc aagtggcccg acgggaactg ctatgcgcag
     2461 gttcgtgacc cggccgggga caacatagaa ctggcgacgg caatcatgga tcgttacatc
     2521 caggaatatc cggccgacac tctcgggtgg tcctgggaac cgaccgtcct gatgaccggg
     2581 gtgtatgaat tgtatcgagt caccggtcat gagcgcttcc ttcactacat gcgcgactgg
     2641 atggatgcca acctggaaat cggttacttc atgttctgtt ccgactttgc gtcgccggcc
     2701 ctgattgcgg ccttccttta catggaaact ggcgaggaac gttatctggc ggtcgtccag
     2761 gatgccttta cctatctcga ggaaaccgtc ataagaacgg acgacggcgg gtttaaccac
     2821 tggggcgagg cggaattcaa cggcggcgcg accttgtggc tggacacgct tttcatggtc
     2881 ggtacgatgc tgaccaggtg gggcgaattc gttaatgatt cgggggcgct tgacgaaatg
     2941 ggccttcaga ttgccgtctt cacggcacgg cttcaatcgg aaggcggcct gtatgttcat
     3001 gcgtttaatt acaacaagcc tgtcgatacc gatatctact ggggacgcgg gaatggctgg
     3061 gtaagcgcgg ccggtttcga ttatcttcgg gcccgtcttg tccgtggcga gcgcgatgtt
     3121 gtcgccgaag aggcgcttgt cgcccaggca caggccgcgc ggttcgccca ggatcaggaa
     3181 agcgggcttt ggtggatcgt gctgaaccgg cccggcgagg cttacgtcga gacatcgacg
     3241 gccgcgctgt tcgcctatgg catggcgcga ggctggcgct atggccttct gtttgacgaa
     3301 tttctgcccg tcatcaagcg cgcgatggat ggaatccgcg ccacgattac cactgatgat
     3361 ttgggacgtc cggttgtttc gaacgtgtcg gggcccacgg aagccgggac ttttgaatac
     3421 tacgcaggcc tgccggtcgc cgatgacctt ggctacgggg ttggtgcggt cgtcctggcg
     3481 cttgtggaaa cgtcgggcct tccgattgag taatgcatgt ctatttgccg atgaagccgg
     3541 ccgcgaccag cgcgatcgta agcgccgcga ttcccagcct gtaccagccg aagatcctca
     3601 tgccgtgcct ggacaggtaa tagaccatcc acttgaccgc gcccaacgcc gacacgaagg
     3661 aaactatcat cccaataaga agcggttccc atccgaattc agcagtcagt tcaccaaaat
     3721 ggcttgtcag atcccagacc gtcgcggccc caagcgttac aacgccaagc aggaatgaga
     3781 actcgacggc ggaaggaagc gacaaaccga cgaccagggc gccgatgatc gtgacaagac
     3841 tgcggctggt tcccgggaca agagccagaa gctggaaaag tccgatggtc aatgcacccc
     3901 gccacgtcag ggtctcaaga ggttgcccga ttgacggatc acagaagcga gggttcatga
     3961 agataacgag gatggccagc ccccagagtg cccaggcccc ggcgacgata ttaaggttga
     4021 aaaggtaagt cttgatgaaa tccccggcca gaaatccggc gacagccgcg ggtacgaacg
     4081 caaccagaac gttcagcgcc aggttgcggc cctcgtcgtc gcgtccgacc aacccctttg
     4141 ccatctggga gacgcggctg ccgtaaaccg ccaggatcgc caggatcgcg ccaagctgga
     4201 ttgcgatggc gaaagagtcg gcggcggacg tccgtgggat gttcatcagg cgctcggcca
     4261 gcagcaggtg accggtcgaa ctgaccggaa ggtattctgt gattccctcg acgacgccaa
     4321 gaacggccgc ctgccacgac tgcatggaaa aacctccgcc gtggtggtag tgtacgcgct
     4381 aacccgatcc gcgacaatcg gggcggggac ttgcttgttt attcgaccgg aggtccaatg
     4441 agaatcctgt ctggcataca gccgtccggc cggctgcata tcggtaatta tttc
//
LOCUS       DAOPPA010000257         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21062_length_4485_cov_3.707901, whole genome shotgun sequence.
ACCESSION   DAOPPA010000257 DAOPPA010000000
VERSION     DAOPPA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21062_length_4485_cov_3.707901"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKH54_08590"
     CDS             <1..741
                     /locus_tag="PKH54_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC99990.1"
                     /translation="MSWGGRKEPDRFEFDVVVARPMEIAITKDTVDLEKGTITFTGSE
                     PVAKVTLEIRGESGDELLSKEFVVKSEAGQPTTISFTPPTEPIGRVDLLAHDPWGFYN
                     GIQMSPFFVQVPNSKEVIFESAKWDILPAEEPKLVEVLDRVFAALNKLGAGFQANLYV
                     AGYTDTVGSNESNYVLSEKRAKSISQWFRNNGLHIRTCYQGFGEDSLAVKTDDNVDEA
                     RNRRTIFVIASQQPPKNATFPRGNWKCL"
     gene            831..1382
                     /locus_tag="PKH54_08595"
     CDS             831..1382
                     /locus_tag="PKH54_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC99991.1"
                     /translation="MTTSKLRGFTLVELMFVVAILGVLSVVAVASYRGYMKKAYSQEA
                     IAFLLDIKMKQETYFMTYSQYVDTGATPEDMFPALNTIPDFWLQGGLQWDCANPPNPA
                     ITGFCALGIVPKQEMTHYQFVTMGWAPGDLVPAADARGPWIQNPARRWWFARARSWTD
                     LTKTLTLEIRVNSEVNEVIELTY"
     gene            complement(1492..2985)
                     /locus_tag="PKH54_08600"
     CDS             complement(1492..2985)
                     /locus_tag="PKH54_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99992.1"
                     /translation="MPHNGRFAFMFFDTGGRPQSGFCPVMSARGIPVCESFSRVLAAV
                     SVLAALAMFQAPAQAQTQPAGQPAPAAQQPQGKMLQIKDIKLPSASELQPDSSGLVMA
                     NGLEPDVTADVSRVLGLLKRYRGLELKTPVKATRTTALQLLEYVNRQLNKPESARQVQ
                     VVEAFFKATGSIPADTDLTGIMRSYLVEEIAGLYDWEDKTLYLAERVYGDLRTLTMSH
                     ELTHAMQDQHFGLSRFMDIREGVSEPFAAAQALMEGDATLTMLQVQTGTVPNPMVAEL
                     LVKSLFSVLDMDGRMKELKVPPVIGELLMFPYLAGFRFAVHFVSQANGKWADIDWMFM
                     NPPLSTEQLMHPEKMHRPDAKSSPDFPRDVVIPGAAELVTGAAFHGSDIAGEGNLAAL
                     FRHGSNPGAAAEAASGWDGDRFVVWRDKAGRHGLVIASVWDSPDDAAQAAQAFRETPV
                     RPAAVKVSGDRVVAAWGSAVKSPEKMVSRILSDFSTRDIRRWQDWFR"
     gene            3143..3628
                     /locus_tag="PKH54_08605"
     CDS             3143..3628
                     /locus_tag="PKH54_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOC99993.1"
                     /translation="MDKKLYEEMNKQVQEEFYAAYLYLSMSNWLEDQNFGGFAQWMKA
                     QAHEETEHAMKIRDFLIDVGQKVNLMPIAKVPTEFKSVLDCFEGALAHEKHVTSRINM
                     LYGLAKDKKEYAAEVFLQWFVKEQVEEEKQTADAVAALKMVGESMGALLQLNKTYGKR
                     D"
     gene            3863..>4485
                     /locus_tag="PKH54_08610"
     CDS             3863..>4485
                     /locus_tag="PKH54_08610"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOC99994.1"
                     /translation="MARDIAKVLGADRKGQGYLEGGNWIVTWAIGHIVTIAEPHEMNP
                     DWKRWAWNRLPILPTEWMLKVIQQTADQFEVVKRLLCRDDVAGVVCATDAGREGELIF
                     RYVYQMSGCGKPFKRLWISSLTPDAIKAGFKALRDGRDFDNLGLAAVARARADWLVGM
                     NLSRGYSLRFGSRDGENRLLSVGRVQTPTLAMLVDRELAIQDFRAEK"
BASE COUNT          971 a         1292 c         1325 g          897 t
ORIGIN      
        1 atgtcctggg gtggccgcaa ggaaccggat cggttcgaat tcgacgttgt cgtcgcccgc
       61 ccgatggaaa tcgcgatcac gaaggacacg gttgaccttg aaaagggaac catcaccttc
      121 accgggtcgg aaccggtcgc gaaggtcacg ctggaaatcc ggggcgaatc cggggacgaa
      181 ctgctgtcga aggaattcgt ggtgaaatcc gaggccggcc agccgaccac aatcagcttc
      241 acacccccta ccgaaccgat tggacgcgtg gatctgcttg cgcacgaccc atggggattt
      301 tacaacggca tccagatgtc gcccttcttc gtccaggttc caaattcaaa ggaagtcatc
      361 ttcgaatccg cgaagtggga cattcttccc gccgaggaac cgaagcttgt cgaggttctg
      421 gaccgcgtgt tcgccgcact taacaagctg ggggccggct tccaggccaa cctgtacgtg
      481 gcgggataca ccgatacggt cggcagcaac gaatccaact atgtcctgtc ggaaaagcgc
      541 gcgaaatcca tttcccaatg gttccgcaac aacggtctgc acatacgcac gtgctaccag
      601 ggtttcggcg aggactcgct ggcggtcaag accgatgaca acgttgacga ggccaggaac
      661 cgccgcacca tattcgtaat cgcctcccag cagccgccga agaacgcaac ctttccccgt
      721 ggcaactgga agtgcctgta gggaagatgg caagcttgcc gctttgtttg acccgggaac
      781 gccgtggcca tagaatatgc cggtatggtc gtcacccggg ggaacttctg atgacaactt
      841 caaaactccg tggattcacg cttgtcgaac tgatgtttgt cgtcgccatc cttggcgttc
      901 tttccgtggt tgccgtcgca agttatcgcg ggtacatgaa gaaggcctac tcccaggagg
      961 cgattgcatt cctgctcgac atcaagatga agcaggaaac gtacttcatg acctattcgc
     1021 agtacgtcga taccggggcg acgccggagg acatgttccc ggcgcttaac accattcccg
     1081 atttctggct gcagggcggc cttcagtggg actgcgccaa tccgccgaac ccggcaatca
     1141 ccgggttctg cgccctggga attgtcccga aacaggaaat gacccattac cagtttgtta
     1201 cgatgggttg ggctcccggt gacctggttc cggccgccga cgcccgcggg ccgtggatcc
     1261 agaaccctgc cagacgttgg tggttcgccc gcgcaagaag ctggaccgac cttaccaaga
     1321 cgctcaccct ggaaatccgg gtcaacagcg aagtcaacga agtcatcgag cttacttact
     1381 gaccttccgg ccttcggctc tctgcgttcc ggcattaaaa ttcacacgat catgttccga
     1441 cgtgttgtcc agggatgacg catgtctcac gtaacgggga cttcgtgcgg gtcaacgaaa
     1501 ccagtcctgc catcttcgaa tatcacgggt ggaaaaatcg gaaaggattc gcgaaaccat
     1561 cttttcgggc gatttgaccg cgcttcccca ggcggcgacc acgcggtcgc ccgaaacctt
     1621 gaccgcagcc ggccgtaccg gggtttccct gaaagcctgg gcggcctggg ccgcatcgtc
     1681 cggtgaatcc cacacggacg caatcaccag cccgtgtcgc ccggccttgt cccgccacac
     1741 aacgaaacga tcgccgtccc agccggatgc ggcttcggcc gccgcgcccg ggttggaacc
     1801 atgacgaaac aacgcggcca ggttgccttc cccggcaatg tcacttccgt gaaaggccgc
     1861 cccggtgaca agttcggccg cgccgggaat cacgacgtcg cgcgggaaat cggggctgga
     1921 cttggcgtcg gggcggtgca tcttttccgg gtgcatcaac tgctcggtcg aaagcggggg
     1981 attcatgaac atccagtcga tgtccgccca cttcccgtta gcctggctga cgaaatgcac
     2041 cgcgaatcgg aacccggcaa gatacgggaa catcagcagt tcaccaatca cgggcggaac
     2101 cttcagttcc ttcatccgcc catccatgtc caggacggaa aacaggcttt tgaccagcag
     2161 ttcggcgacc attggatttg gaacggttcc cgtctgaacc tgaagcattg taagcgtggc
     2221 atccccttcc atcagtgcct gggcggccgc aaacggctcg gatacgcctt ccctgatatc
     2281 catgaacctg gacagaccga aatgctgatc ctgcatggcg tgggtgagtt catggctcat
     2341 ggtcagcgta cgcagatcgc cgtaaaccct ttcagcgaga tagagcgtct tgtcttccca
     2401 gtcgtaaagt cctgcgatct cttcaacaag gtatgaacgc attattccgg tcagatcggt
     2461 gtcggcggga atcgagccgg tcgccttgaa aaaggcctcg acgacttgca cctgccgggc
     2521 cgactcgggc ttgttcaact gacggttcac gtactcaagc aactgcagcg ccgtggttcg
     2581 cgtcgccttg acgggcgtct tcagttccag gccgcgatat ctcttcagca gtccaaggac
     2641 gcgggacacg tcggccgtta cgtcgggctc gagcccgttc gccatcacga gtccggacga
     2701 atccggctgc aattcggaag cggacggaag cttgatatcc tttatttgaa gcatcttgcc
     2761 ctggggctgc tgtgccgccg gcgctggctg gccggcaggc tgtgtctggg cctgagccgg
     2821 cgcctggaac atggccaggg cggccagcac ggaaacagcg gcaagcacgc gggagaaaga
     2881 ttcgcaaaca gggatgcccc tggcagacat gacgggacaa aagcctgatt gcggccttcc
     2941 gcccgtgtcg aagaacatga aagcgaaacg tccgttatgt ggcatggtta cctctttcaa
     3001 aacaaaaagt tccgttttac ccacttaaca aaaaggtttg cggtgtttat tgtactccaa
     3061 acgggaacaa ccgggacggt cccggcgttc cgcgcatcat gcttatgcgc cgttaatggc
     3121 ttaaatatct ggaggatcga tcatggacaa gaaactgtat gaagaaatga acaagcaggt
     3181 tcaggaagaa ttctatgccg cttacctgta cctttcgatg tcgaactggc ttgaagacca
     3241 gaatttcggc ggctttgccc agtggatgaa ggcccaggca cacgaagaga ccgaacacgc
     3301 gatgaaaatt cgcgatttcc tgattgatgt cggccagaag gttaacctga tgccgatcgc
     3361 caaggttccg accgaattca agtcggtgct ggactgcttc gagggcgccc tggcccatga
     3421 aaagcacgtc accagccgca tcaacatgct gtacggcctg gccaaggaca agaaggaata
     3481 tgccgccgaa gtgttcctgc agtggttcgt gaaggaacag gtcgaggaag agaagcagac
     3541 cgccgacgcc gtcgccgccc tgaagatggt tggcgaaagc atgggtgccc tgctgcagct
     3601 taacaagacc tacggtaaac gcgactgatc gccgtaaatc aagccgttcc acgccggggt
     3661 gtccgtaagg cttgaatgcc gctggtttgc cgccctctgg tttggctgat acaacctgcc
     3721 gcaatacttc gaagaatcac accggcacaa agcattgctt tgaacgcggt tcggtaaggg
     3781 ttaaaatccg gccgttgaac aggtttcgcc acgattcaag gagccccgtt tgttcacagt
     3841 cgtacttgca gaaaagccgg cgatggcgcg tgacatcgca aaggttctgg gtgcggaccg
     3901 gaagggtcag gggtatctcg agggcggcaa ctggattgtg acatgggcca tcggacatat
     3961 cgtcacgatt gccgagccgc acgaaatgaa cccggactgg aagcgatggg cgtggaacag
     4021 gctgccaatc ctgccgacag agtggatgct gaaggtgatt cagcagactg ccgaccagtt
     4081 cgaggtggtc aagcgactgc tgtgccgcga cgatgttgcc ggtgtcgtgt gcgccaccga
     4141 cgccgggcgc gaaggcgagc ttattttccg ttatgtctat cagatgtcgg gatgtggcaa
     4201 acctttcaag aggttatgga tctcgtccct gacaccggac gcgatcaagg ctggcttcaa
     4261 ggcgctgcgc gatgggcggg actttgacaa tctgggcctg gcggcggtgg cgcgggcgcg
     4321 ggccgactgg ctggtcggca tgaacctgtc acgcggttac tcgctgcggt tcgggtcgcg
     4381 ggatggcgaa aaccgccttc tttcggttgg acgtgtccag acaccgacgc tggcgatgct
     4441 tgtcgatcgc gaactggcca tccaggattt ccgggccgaa aagta
//
LOCUS       DAOPPA010000258         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21069_length_4484_cov_3.336193, whole genome shotgun sequence.
ACCESSION   DAOPPA010000258 DAOPPA010000000
VERSION     DAOPPA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21069_length_4484_cov_3.336193"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKH54_08615"
     CDS             <1..628
                     /locus_tag="PKH54_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC99995.1"
                     /translation="EKKCWDARLAVIGQVGSLENSASDGGDALHGAWGVVASGGVNLK
                     HFELKASGTVLSADDDSNGNDQLAFMWSGKRPGMSILLSENESRDLGNNFDERIASRD
                     GGFNTTVAGMGALDLGLYYKPVDYMKLGFVTAALFVLNPSNALGGSFVGSENELVFDA
                     ELMHDLIRIQAIAGLIVPGAAGAALVNSIDLKARDNIYFGQLAVILNF"
     gene            744..2900
                     /locus_tag="PKH54_08620"
     CDS             744..2900
                     /locus_tag="PKH54_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC99996.1"
                     /translation="MPKKVREWTLLEEVGRGGMGVVWRARHMILPGDWAVKVIRPELS
                     SDAETRDRFLSEVTVLKRLRHPNVVEVDSPFQEGGELYLPMEFLAGQSLEKVLAARPG
                     SWEPAAAVAIIRQAAAGIGFGHRQKPPVLHRDVKPGNIHILSDGRVKVVDFGLARTLG
                     EKSTTSRGLAVGTPAYMAPEVLDGKRATPQSDVYGLGIVLYRLLAGRMPFNMPDEDSA
                     SAMAIVLAVSRAHASGLVDVRQYAPAVSSDLAGVVMSALAADPSERPADAAEFESRLA
                     RLYPSPAQSSPSVQSRPGMSVMAASPAAVDYDATSLGIQVSSGSSQEQGRAAWAFSAP
                     SEQSGHSPSGRVERPMAGAGFVPGCPGVPDILAGIRIELPAPDDSPDVRRRGGSGSRA
                     AAGMPGWVPSWMRGALSVVIGMPGRIYDRLPPGIAGLLTLRNLIIVGVAMVVVAGGIV
                     GFTMYQSWKEARDEAARQAAVVVAPEPPSPDKIAGINWVRIEGGGFWMGSSTGQSDEQ
                     PTHFVTVKSFSMSATEVTIEQYQKCVDAGACSRPHFDDGTCRVYIDGVWSSRVLPQPF
                     RGASNPVVCVDWEQASAFARWVGGRLPSEAEWEFAARAGVTADLYGSPGDIAWYRDNS
                     YGSTSAVGRKRPNANGLYDMIGNVWEHCEDVYHNSYSGAPSDGSARRYPSGKFRVNRG
                     SSWDDGASVLRLSNRYRLEYGKSSCSLGIRVVRDVI"
     gene            2914..>4484
                     /locus_tag="PKH54_08625"
     CDS             2914..>4484
                     /locus_tag="PKH54_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC99997.1"
                     /translation="MSISVREWVLIDEIGRGGMGIVWRARHQMLPGDWAVKVIRPELS
                     QDSESRARFLSEVTVLKRLRHPNIIEVETPFEEGGNLYLPMELLTGKSLEKLLLERPH
                     PWNPQDVVNLARQAAAGLGYAHTQAEPVLHRDVKPGNIHVLDGGRVKILDFGLARTLG
                     DRSMTAAGKAVGTPAYMAPEVLDGKRASTRSDVYALGVVMYRMLTGRLPYDLPEDESS
                     LVALVLSIVRAHDRGLPDIREFAPAVPPQLAAAVMNTLSRDPALRPADGMELSASLSR
                     RDLLSSQVGDGQAGLDSTRLGIELPATGRAAQAPASPAGVPITPPEAFGRPIGSHVPA
                     QRDADVSLLGIRVTAGDSGVKSEPDTDRFAEKSDFVPVVPQVVHAKPPRGRKIWPWIV
                     LGVVGIAIGAGFGLFGIGSGESGPLSSSGLLSGGEPLEKVQFSMHIMSQCPFGAQVQK
                     GIAPVLKTLGKAVDFKMYYIGDEPEPGNLTSMHGENEVAGDKLQLCAMEYAPANYMDV
                     VVCMANDMRNIPQNF"
BASE COUNT          839 a         1268 c         1449 g          928 t
ORIGIN      
        1 tgaaaagaag tgctgggacg ccaggctggc cgtcatcggc caggtcggaa gccttgaaaa
       61 ttcggcctct gacggtggcg atgcgctgca tggggcgtgg ggcgtggtcg cttccggcgg
      121 cgtgaacctg aagcattttg aacttaaggc ttcgggaacc gtcctttccg cggatgacga
      181 ctcaaacggc aacgaccagc ttgcctttat gtggtccggc aagcgccccg gcatgtcaat
      241 cctgctttcg gaaaacgaat cccgcgacct ggggaacaac ttcgacgaaa gaatcgccag
      301 ccgggatggt ggattcaata cgaccgtggc cggcatgggt gcgcttgatc tggggcttta
      361 ttacaagccg gtcgattaca tgaaactggg cttcgtcacg gcggcccttt tcgtgttgaa
      421 tccatcgaac gcgcttggtg gatcattcgt cggcagcgag aacgaactgg tatttgacgc
      481 cgaactgatg catgacctga ttcgcatcca ggccatcgcc ggcctgatcg tccccggcgc
      541 cgccggcgct gcgctggtta acagcatcga cctgaaggcc agggacaata tctacttcgg
      601 ccagctcgcg gtcatcctga acttctgacg cgactgcaaa acactgtccg aacacccacg
      661 tcgcagcaaa ttattacgtg aaaaagtgtg accctttgtg ataaaactgc accgaaccaa
      721 aagagtccgg taaggagggc aggatgccga agaaggttcg ggagtggacg cttctggagg
      781 aggtcggacg aggtgggatg ggcgtggtct ggcgggcccg tcatatgatt ctgccgggcg
      841 actgggccgt gaaggtcatc cgtcccgaac tgagttcgga tgcggaaacc cgtgatcgct
      901 tcctttccga ggtgaccgtc cttaagcggt tgcgtcatcc caacgtggtc gaagtcgatt
      961 cgccgtttca ggaaggtggc gaactgtacc ttccgatgga attccttgcc ggacagtccc
     1021 tggaaaaggt gcttgcggca cgtcccggtt cgtgggagcc cgcagccgcg gttgccatca
     1081 taaggcaggc agcggccggc atcggtttcg ggcacaggca gaagccaccg gttcttcatc
     1141 gtgacgtgaa gccgggaaat attcacatcc tttcggatgg ccgggtgaag gttgtcgatt
     1201 tcggtcttgc cagaactctt ggcgagaagt ccaccacgtc gcgcggcctt gcggttggca
     1261 cgccggccta catggcgccg gaggtgcttg acggaaagcg cgccacgccg cagtccgacg
     1321 tttacgggct tggaatcgtg ctttaccgcc tgctggccgg aaggatgccg ttcaacatgc
     1381 cggacgagga ttcggcctcg gcaatggcga ttgtgcttgc cgtatcgcgg gcccatgcat
     1441 cgggcctggt cgatgtccgt cagtatgccc cggccgtgtc gtcggatctt gccggcgtcg
     1501 tgatgtctgc cctggccgcc gatccgtctg aacgacccgc ggatgccgcc gagtttgaaa
     1561 gtcgcctggc caggctctat ccgtcccccg cccagtcaag tccgtccgtt cagtcccgtc
     1621 cgggcatgtc ggttatggca gcatcacccg ccgcggtcga ttacgacgcg acgtcgcttg
     1681 gcatccaggt ctcatcggga tcaagccagg aacagggcag ggccgcctgg gctttttcgg
     1741 caccttcgga acagtccggt cattcgccca gcggacgtgt tgaacggccg atggccggtg
     1801 cgggtttcgt tcctggatgt ccgggtgtcc ccgatatcct tgcgggcatt cggattgaac
     1861 ttccggcgcc cgatgattcg ccggatgtcc gtcgtcgcgg cggatcgggc tcgcgtgccg
     1921 cggcgggcat gccgggctgg gtgccgtcgt ggatgcgtgg cgcgctgtcg gttgtcattg
     1981 gtatgccagg tcggatttat gaccgacttc cccccgggat tgccgggctt ctgaccttgc
     2041 gcaacctgat tatcgtcggt gtggcgatgg tggtcgtcgc gggtggaata gtcggcttca
     2101 ccatgtatca atcctggaag gaagcccgtg acgaagccgc gaggcaggcc gctgttgtgg
     2161 ttgccccaga gcccccttcg cccgacaaga tcgccggaat caactgggtc aggattgaag
     2221 gaggcggttt ctggatgggt tcatccacgg gacaatcgga cgagcagccg acgcatttcg
     2281 tgaccgtcaa gagcttcagc atgtccgcga ccgaggtcac gatcgaacag taccagaagt
     2341 gcgtcgatgc cggtgcgtgc agccgtccac atttcgatga cggaacgtgc cgcgtctata
     2401 tcgacggggt ctggtcttcc agggtgcttc cccagccgtt caggggtgcg tccaacccgg
     2461 ttgtgtgcgt tgactgggaa caggcttccg cttttgccag atgggttggc ggcaggcttc
     2521 caagtgaagc cgaatgggaa ttcgcggcca gggccggcgt gacggccgac ctttacggca
     2581 gtcctggtga catcgcctgg tatcgagaca attcatacgg aagcaccagt gcggtcggac
     2641 gcaagcgacc gaatgccaac gggctgtacg acatgattgg caacgtctgg gagcattgcg
     2701 aggacgttta ccataacagc tattccggcg cgccgtccga cggaagcgcg cgcaggtatc
     2761 catccggaaa gttccgggtc aaccgcggct caagctggga cgacggtgcc tcggtactcc
     2821 gcctgtcaaa caggtacagg ctcgagtacg gcaagagcag ttgttcactg gggattcggg
     2881 tggttcgcga cgtgatctga tagagggaac aagatgtcga taagcgtaag ggaatgggtt
     2941 ttgatcgacg agattggccg gggcggcatg ggcatcgtct ggcgtgccag gcatcagatg
     3001 cttccgggcg actgggccgt aaaggtcatt cgtcccgaat tgagccagga ttccgaatca
     3061 agggcgcgct ttctttccga agtcaccgtt ctgaagcgcc ttcgacatcc aaacatcatc
     3121 gaggtcgaga cgccgttcga ggaaggcgga aacctgtatc tgccgatgga gcttcttacc
     3181 ggaaagtcgc tggaaaagct gcttctggaa aggccgcatc catggaatcc ccaggatgtg
     3241 gtcaatcttg cgcgccaggc cgcggccggt cttgggtacg cccataccca ggcggaaccg
     3301 gtgctgcaca gggacgttaa gccggggaat atccatgtcc ttgacggcgg ccgcgtcaag
     3361 atcctggatt tcggtctggc caggactctt ggcgacagat cgatgacggc ggcgggcaag
     3421 gcggtcggga cgccggcata catggccccg gaagtccttg acgggaaaag ggcatcgaca
     3481 cgatccgacg tgtacgcgct tggcgtggtc atgtatcgaa tgcttaccgg aaggctgccg
     3541 tatgaccttc ctgaagacga gtcctcgctt gtcgccctgg ttctttcgat tgtccgcgcg
     3601 catgatcggg gattgcccga tataagggaa ttcgcgccgg cggtgccccc gcaacttgcg
     3661 gccgccgtga tgaatacgct ttcccgcgac ccggccttgc gccccgcaga tggaatggaa
     3721 ctgtctgcca gcctttctcg gcgggatctt ctttccagcc aggtcgggga tggacaggcc
     3781 ggccttgatt cgacaaggct cgggattgaa cttccagcca ccggccgggc cgcgcaggcc
     3841 ccggccagcc cggcgggcgt gccgattaca ccgcctgaag ccttcggaag gccgattgga
     3901 tcgcacgtcc cggcgcagcg tgatgccgac gtcagtctgt tggggattcg ggtaaccgcg
     3961 ggtgattccg gcgtcaaatc agaacctgac acggacaggt ttgccgaaaa gtcggatttc
     4021 gttccggtgg ttccccaggt cgtccacgcc aaaccgccac gtggccggaa aatctggcca
     4081 tggattgtcc ttggtgttgt cgggattgcg atcggcgcgg gtttcgggct gttcgggatc
     4141 gggtccggcg aatccggacc cttgtcgtcc agcgggttgc tttccggcgg tgaacctttg
     4201 gaaaaggttc agttttcaat gcacattatg tcccagtgtc cattcggtgc gcaggttcag
     4261 aagggtatcg ccccggtgct gaagaccctc ggaaaagcgg ttgatttcaa aatgtattac
     4321 atcggtgacg aaccggaacc gggcaacctg acatcgatgc acggcgaaaa tgaagttgcc
     4381 ggggacaagc ttcaattgtg cgcaatggag tatgccccgg ccaactacat ggacgtggtc
     4441 gtctgcatgg ccaacgacat gcgcaacatc ccgcagaatt tcga
//
LOCUS       DAOPPA010000259         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21261_length_4453_cov_4.189859, whole genome shotgun sequence.
ACCESSION   DAOPPA010000259 DAOPPA010000000
VERSION     DAOPPA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21261_length_4453_cov_4.189859"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..591
                     /locus_tag="PKH54_08630"
     CDS             34..591
                     /locus_tag="PKH54_08630"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HOC99998.1"
                     /translation="MNSVPKILSIRGFIVAAALVVAASFVMTSTVAVALNKGDTVSTV
                     TVKDSKDKDAQIPDIGSKVLAIFYTDPDVQNQNEPFRELLNKSGLDLAKFRGLGVVNM
                     KDTLLANAIIRSVVRGKEKKFNSLILTDPSHLLKNAWNLGDANGKDIVIIIGKDKKVH
                     YIKAGKMTEAEQQKGLELIRELCAR"
     gene            complement(625..2187)
                     /locus_tag="PKH54_08635"
     CDS             complement(625..2187)
                     /locus_tag="PKH54_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOC99999.1"
                     /translation="MEKKLKLQIAAYAVVAVVTAVATVIIAALLVNIFDRKQEAKNPF
                     LKVVEVTEDDVDPAKWGMNWPRQYDGYLRTSEPTATKYGGGGVGPEGGLPPQKAERDP
                     WLTRVFAGYLFAVDYRDRRGHAFMLEDQEITKRNVPGEDKQSGNCLHCHSSIMPLYRD
                     LGEKELPEADKYDQEHKGLELVSQMGYWDAHAMLEKTTGGKAHPVSCVDCHDPESMEL
                     RVTRPGFIGGIRKLAESDSPVPHLSSIDAWRRGNRDKPYDPNTDGTRQEMRSFVCGQC
                     HVEYFCGKGMTLFFPWDKGLKVEEIESMYNGMQVQGKGFKDWVHAETGMPVLKAQHPE
                     FEIWSQGIHARSGVGCADCHMPYKREGALKVSEHWVRSPLLGLNRSCATCHPYGDQEL
                     KDRVEAIQDRHFALMTRAGESAVAMLDAIVALKKTYTDQAWADAILADPQVRKIAELQ
                     RAAQWRLDFVAAENSMGFHAPQETARILAESIDMSRQAQVLAASLLPPPPPVETAAVE
                     PAAAPQPEDRAN"
     gene            complement(2198..2695)
                     /gene="nrfH"
                     /locus_tag="PKH54_08640"
     CDS             complement(2198..2695)
                     /gene="nrfH"
                     /locus_tag="PKH54_08640"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOD00001.1"
                     /translation="MKFRWSRALLALSILVGALGGVGLFTFSYANGPAYLGNDPATCA
                     QCHIMNDQFDSWRKGPHHASATCNDCHLPPQFPGKYVAKGLNGYHHSMGFTFQSPTPD
                     EPGARTVFHEPIRIKDFNSQILQDNCLRCHGDMVHDIVRGSTWADDAIRCVHCHSAVG
                     HGARR"
     gene            complement(2880..>4453)
                     /locus_tag="PKH54_08645"
     CDS             complement(2880..>4453)
                     /locus_tag="PKH54_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOD00002.1"
                     /translation="QTVLRECLLAGLLASDAEIVKDDGQFKAVGDPTEAALVAAAQKT
                     GLDPQKVRLDMPRLDTIPFESQHQYMATLHDRGPDRPGIVYIKGSLEKILERCRVAMS
                     PDGSAELDTDAIRRAADDMAAQGLRVLAFGVKELPAGTRRIDHHDVEQDAVFLGIQAM
                     MDPPRPEAAAAIAACRKAGVLVKMITGDHAVTAAAIAGQLGLGRGNSAAPKALTGREL
                     DALSDSEFIDAAEDVDVFARVSPEHKLRLVEALQARGNIVAMTGDGVNDAPALKQANI
                     GIAMALNGTEVAREAADMVLTDDNFATIEAAVEEGRAVFDNLRKFIVWTLPTNGGECL
                     VLLIAILFGITLPIQPLHVLWINMTTAAVLGLSLAFEPKEPGLMNRPPQDPKNPILDS
                     VLIGRILVVSAVLCASAFAMFEWQIARGFSLAEARTAAVAMIVCGEIAYLFNCRSLTR
                     PIFSIGLLSNPWTLAGAGLMIVLQLAFTYVPVMNRVFESAPIDIIAWGGIAGFSLLLA
                     AIVAIEKMVRGKSRK"
BASE COUNT          835 a         1356 c         1329 g          933 t
ORIGIN      
        1 tccccctgta aacatgaccc aggagggctt ttcatgaact cggtacccaa gattctttcg
       61 attcgcggct tcatcgtcgc ggcagcgttg gtcgttgcag catcgttcgt aatgacctcg
      121 accgtggcgg tcgcgctgaa caagggcgat acagtcagca cggtcacggt caaggacagc
      181 aaggacaagg atgcccagat tccggacatc ggcagcaagg ttctggcgat cttctacacc
      241 gacccggacg tccagaacca gaacgaacct ttccgcgagc tgctgaacaa gtccggcctg
      301 gatctggcga agttccgcgg tcttggtgtc gtgaacatga aggacacgct gcttgcaaac
      361 gcgatcatcc gatcggtcgt tcgcggcaag gagaagaagt tcaattccct gatcctgacc
      421 gacccgtcgc acctgcttaa gaacgcatgg aacctgggcg atgccaatgg caaggacatc
      481 gtgattatca tcggcaagga caagaaggtt cattacatca aggccggcaa gatgactgaa
      541 gccgaacagc agaagggcct ggaactgatc cgcgaactct gcgccagata atctgtccgt
      601 tctgttattc aaactcttaa agaatcagtt ggcccggtct tccggctgcg gggcggcggc
      661 aggttcgacg gcggccgtct cgacgggcgg gggtggcgga agcaggctgg ccgcaagcac
      721 ctgggcctgg cgcgacatgt cgatcgattc ggccaggatg cgggcggttt cctggggcgc
      781 gtggaagccc atcgagtttt cggctgccac gaagtccagg cgccactgcg ctgcgcgctg
      841 aagttcggcg atctttctga cctgcgggtc ggcaagaatc gcgtccgccc aggcctggtc
      901 cgtgtaagtt ttcttcagtg ccacgatcgc gtccagcatc gccactgccg attcgccggc
      961 cctggtcatc aacgcgaaat gacgatcctg aatcgcttcg acgcgatcct taagttcctg
     1021 atccccgtat ggatggcagg tcgcgcacga acggttcagc ccaagcaaag ggctgcgaac
     1081 ccagtgttcc gagaccttga gcgcgccttc ccgcttgtaa ggcatgtggc agtccgcaca
     1141 accgacgccg ctgcgggcgt ggatgccctg gctccagatc tcgaattcgg gatgctgggc
     1201 cttcaggacc ggcattccgg tctcggcgtg aacccagtcc ttgaatccct tgccctgaac
     1261 ctgcatgccg ttgtacatcg attcgatttc ttcaaccttc aggcctttgt cccacgggaa
     1321 gaacaaggtc attcccttgc cgcagaagta ttcaacgtgg cactggccgc acacgaacga
     1381 ccgcatctcc tggcgggtgc cgtccgtgtt gggatcgtaa ggtttgtcgc gattgccgcg
     1441 ccgccacgca tcgatacttg acaggtgcgg caccggcgag tcggactcgg caagtttcct
     1501 tatgccgccg atgaatcccg gccttgtaac ccgcagttcc atgctttccg ggtcgtgaca
     1561 atccacgcag gacaccgggt gagcctttcc accggtcgtc ttttcaagca ttgcgtgtgc
     1621 gtcccagtat cccatctggc tgaccagttc cagtcccttg tgttcctggt cgtacttgtc
     1681 ggcttccggc agttcctttt cacccaggtc gcggtacagc ggcatgatcg atgaatggca
     1741 gtgcaggcag tttcctgact gcttgtcttc gccgggcacg ttgcgcttcg tgatttcctg
     1801 gtcttcaagc atgaaggcgt ggccgcgccg gtcccggtaa tcgaccgcga aaaggtatcc
     1861 ggcaaaaacg cgggtcagcc atgggtcgcg ttcggccttc tggggaggca gtccaccttc
     1921 cggccccacg ccgccaccgc catacttcgt ggcggtcggc tcgcttgtcc gaagatagcc
     1981 gtcgtactgg cgcggccagt tcattcccca tttcgcggga tcaacgtcgt cctcggtgac
     2041 ctcgaccacc ttaaggaagg ggtttttcgc ctcctgcttg cggtcaaata tgttgaccag
     2101 caacgcggcg ataatcaccg tggcgacggc ggtgacaacc gcgactacgg cgtacgcggc
     2161 gatctggagt ttgagcttct tttccatatt ttaaccgtta cctccgtgct ccgtggccga
     2221 cggcgctgtg gcaatgcacg caccggatcg cgtcgtccgc ccaagtgctg ccgcgaacga
     2281 tgtcgtgaac catgtcgcca tggcagcgca ggcagttatc ctgaagaatc tggctgttga
     2341 aatccttgat acggattggt tcgtgaaaga ccgtcctggc gcccggctcg tccggtgtcg
     2401 gggactggaa agtgaagccc atcgagtggt ggtaaccgtt cagccccttt gccacgtatt
     2461 ttccagggaa ctggggcggc aggtggcagt cgttacatgt cgccgacgcg tggtgcggtc
     2521 ccttgcgcca cgagtcgaac tggtcgttca tgatgtggca ctgggcgcat gtggcgggat
     2581 cattcccaag atacgccggg ccgttggcgt agctgaacgt gaacaggccc acgccgccca
     2641 gcgcgccgac aaggattgaa agggccagca aggccctgga ccagcgaaac ttcatctggt
     2701 ggcccctttt gtggccggcg ccaggcgtcc ggtgaacttt ctgcggttca tcgtgcgtcc
     2761 atcagcatca gcagcctttt actttaggtt agaggtcagg aattgacaag gatcggaatc
     2821 gtaaaggtcg tgtgcctgcc gaactgcttc tgggcacgag cgacaagggc gcgtgattgt
     2881 cacttcctgg atttgccccg caccattttt tcaatggcca cgattgcagc cagaagcagg
     2941 ctgaatcctg cgattccgcc ccaggcgata atgtcgatcg gggccgactc gaacacgcgg
     3001 ttcatcaccg ggacataagt gaaggcgagt tgcagcacaa tcatcaggcc ggctccggcc
     3061 agcgtccagg gattcgacag cagtccgatc gaaaatatgg gccttgtaag tgaccggcag
     3121 ttgaaaaggt acgcgatttc cccgcagacg atcatggcga cggcggcggt tctggcttcg
     3181 gcaagactga agcctcgggc gatctgccat tcgaacatgg cgaaggcgct ggcgcaaagg
     3241 acagccgaaa caacaaggat tcttccaatc aggacggaat ccaggattgg gttcttcgga
     3301 tcctgcggcg gacggttcat cagccccggc tctttcggtt cgaaggccag ggaaaggccc
     3361 agtacggcgg ccgtcgtcat gttaatccac agtacatgaa gcggctgtat cggcagggta
     3421 atgccgaaca gaatcgctat cagcagcacg agacattcgc ccccgttcgt gggcagggtc
     3481 cagacgatga acttgcgcag gttgtcgaag acggcgcgac cctcctccac cgcggcctcg
     3541 atggtcgcga agttgtcgtc ggtaagcacc atgtccgccg cctcccgcgc cacttccgta
     3601 ccgttaaggg ccatcgcgat cccgatattg gcctgcttca gcgccggcgc gtcgttgaca
     3661 ccgtccccgg tcatcgcgac gatatttccg cgggcctgca gagcttcaac aagccttagc
     3721 ttgtgttcgg gagaaaccct cgcaaagacg tccacatctt cggcggcatc aatgaactcg
     3781 ctgtcggaca gggcgtccag ttcgcgtccc gtcagagcct tcggtgcggc ggagttgccc
     3841 ctgccgagcc cgagttggcc ggcgatcgct gccgccgtga ccgcgtggtc tcctgttatc
     3901 atcttcacca gtacaccggc cttcctgcag gcggcaatcg ccgcggccgc ttccggacgg
     3961 ggcggatcca tcatggcctg aatccccagg aagacggcat cctgctcgac atcgtggtgg
     4021 tcgatgcggc gagtccctgc gggaagttcc ttgaccccga aagccagtac gcgcagtccc
     4081 tgggcagcca tatcgtccgc agcgcggcgg attgcatcgg tgtcgagttc ggcagacccg
     4141 tccggactca tcgcgacccg gcagcgttcg agtatctttt ccagcgatcc ttttatgtac
     4201 acgattcccg gccggtccgg cccccggtca tgcagggtcg ccatatactg gtgctgcgat
     4261 tcgaagggaa tcgtgtccag cctcggcatg tccaggcgaa ctttctgcgg gtcgagtccg
     4321 gtcttctgtg cggccgcaac cagggctgcc tccgtcgggt cgcctacggc cttgaactgc
     4381 ccgtcatcct ttacaatttc cgcgtcgctt gcaagcaggc ccgccaaaag gcattcgcgc
     4441 agaaccgtct gat
//
LOCUS       DAOPPA010000260         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21307_length_4446_cov_3.816898, whole genome shotgun sequence.
ACCESSION   DAOPPA010000260 DAOPPA010000000
VERSION     DAOPPA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21307_length_4446_cov_3.816898"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKH54_08650"
     CDS             complement(<1..1006)
                     /locus_tag="PKH54_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00003.1"
                     /translation="MKHTSPHRLSLVSIFLTVSVVWLTASCTGGVITGDSIDETGGAD
                     TIIQADLPGDGNADPDGAGDTGIVDDVGLDVPVPDSGDAVNDSGDPDSQGFDLGDESD
                     SGDLGQGDADGGALDAADLFQADSGDPDEGRPDSILDDADDADDADDAVDAGVEDDGE
                     QTEDDGGHIDIADDQGHDATIPDSGDDVSVPNCPGEPGCPCEDATDCFSGICIPTNDQ
                     SVCATDCFSKSDCPRGWLCEPVPTGTGAPLVCLYPFENLCRPCNDDSECVPSYGVALL
                     HHSCIDFGPDGSFCGRECLFAWDCPDGYSCQITQTAMGPMSQCRPTDGTCECTTRFIE
                     Y"
     gene            complement(1022..2194)
                     /locus_tag="PKH54_08655"
     CDS             complement(1022..2194)
                     /locus_tag="PKH54_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00004.1"
                     /translation="MRNASKRIAFHSVAVLILLCSAPSAAQLVKKADPSRAGEQKGDR
                     AIDRLVKAVNSAYDTDLKAADVVDGVPAALVEVVRAFYGDQTLKAADKLKAFGAAGKR
                     FDERWAEYTAKKAKVDEILKKVKKLKSSGFETDARDVLINSISPVEREDDGTISRNRQ
                     FLESSDAEMALLTELGHLLEGLKEYGTLFEVAAAMDGRRKVFSVEEERLLWIAAEHDL
                     LKFADGARAPVSDAIGFIVDSVSKTREMGGLLSYGLWQKLNGGMLKRLQPADPDTAQI
                     DDWVIVNLTPDRVDESGASWFKDGSFDVERDCYATSRIVGIDYWTGRVYYQVACRKVH
                     VTLNIRVNVEFATPPDSWARKGNVFIVGRVTGKSRTNGVQWKIEDAFIPDVRFINR"
     gene            2472..>4446
                     /locus_tag="PKH54_08660"
     CDS             2472..>4446
                     /locus_tag="PKH54_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00005.1"
                     /translation="MHQSISRGSVPSLKRIGYIVFAMSALALTGSGCSGSSDECIDSI
                     DCQRGLVCRSGECILLACSPAGGCSDLEVCTPGSYGGNDPNKDFCSARCSVFNKCRAG
                     ERCTDGVCLPDDYVDDIHDVPQDEGSSVDMIGDSGGADSHAGDLQEDARDSDAPDDTP
                     DETDMPDAGDSGPDGDLSLDEGPQDSGPQDVGTDADVEPEVQMVFVPPATFWMGCNQG
                     IDGGCGEGELPYHKVDLSGYYIDRTEVTQGEYDQCVLAGKCSRPACGFNPASTPDKPV
                     VCVTWNQAGMYCSWAGKQLPTEAQWEHAARGTDGRVFPWGNEAPTCELAVMSGCGTSI
                     GKVCTRSPAGDSFHGLCDMAGNAWEWVNDWYGEQYYATSASVDPTGPTSGRSRVARGG
                     SFDNTAGPLRASARSYGKDTFSAENLGFRCALSRGEPEICDGVDNDIDGLTDEDFGIS
                     EWDGTIAGVGQPCGTGACAGGRVLCNEDGIGSICDTSHLISAEIANGIDDDCNGLTDD
                     LPCVPDCSGRECGLDPVCGTMDCGPCSGSDACIDGACVWAPDQYQVLCGADSMCFVPD
                     GVFWMGCNETVDPECGDDERPYHQVGLSAYYIDRTEVTQADYAKCVDAGVCAQPTCNW
                     NPAATGSFPTVCVDWDDADTFCRWAGKRLPTEAE"
BASE COUNT          875 a         1332 c         1312 g          927 t
ORIGIN      
        1 cgtattcgat gaacctggtc gtgcattcac aggttccgtc cgttggccgg cactggctca
       61 ttgggcccat cgccgtctgg gttatctggc aggagtaccc gtcagggcag tcccacgcaa
      121 aaagacattc ccgtccgcag aagctgccgt ccggcccgaa gtcgatgcag gaatggtgaa
      181 gaagagcgac gccatagctc gggacgcact cgctgtcatc gttgcagggg cggcagaggt
      241 tttcaaatgg atacaggcat acaagcggag ccccggttcc agtcggaacc ggttcacaca
      301 accagcccct gggacagtct gacttcgaaa aacagtcagt cgcgcacacg gactggtcgt
      361 tcgtcggtat gcagattccg ctgaagcagt cggtggcgtc ttcacatggg cagcctggct
      421 cgccggggca gttcgggacg gacacatcgt cacctgaatc cgggatggtg gcgtcatgtc
      481 cctggtcgtc ggcgatgtcg atgtggccgc catcgtcttc ggtctgttcg ccgtcatctt
      541 caacgccggc atcgacggca tcgtccgcat cgtcggcatc atcggcatcg tcaaggatgg
      601 aatccgggcg cccctcgtcg ggatcgccag aatcggcctg gaacaagtcc gccgcgtcca
      661 gggcgccgcc atccgcgtcg ccttgcccga gatccccgga atccgactcg tcacccaagt
      721 caaaaccctg ggaatcgggg tcaccggaat cgttgacggc gtcgcctgaa tcgggaaccg
      781 ggacatcaag cccgacatca tccactattc ccgtgtcacc agccccatcc ggatcggcat
      841 tgccgtcgcc gggcagatca gcctggataa tggtgtccgc accgccagtt tcatcgatgc
      901 tgtcgccagt tatcacgccg ccggtacatg acgcagtcag ccaaacgaca gagactgtca
      961 ggaaaattga aacaagggac agtcggtggg gtgatgtgtg cttcatgaaa tttctccgaa
     1021 atcagcggtt gataaaacgt acgtcgggaa tgaaggcgtc ctcgatcttc cactggacgc
     1081 cgtttgtcct gcttttgccg gtgacgcggc cgacgatgaa gacgttgccc ttacgggccc
     1141 aggagtccgg cggggtcgca aattcaacgt taacccggat gttgagggtg acgtgaacct
     1201 tccgacaggc gacctggtaa tagacgcgtc cagtccagta gtcgatgccg acaatcctgc
     1261 tggtcgcgta gcaatccctt tccacgtcga atgatccgtc cttgaaccag gacgcgccgg
     1321 attcatcgac gcggtcgggt gtcaggttga caatgaccca gtcatcaatc tgggccgtgt
     1381 ccgggtcggc cggttgaagc cgcttcagca tgccgccgtt caacttctgc cacagtccgt
     1441 acgacagcag tccgcccatt tccctggtct tcgatacgga atccacgatg aagccgattg
     1501 cgtcggaaac cggtgcccgt gcgccatccg cgaacttcaa cagatcatgt tccgcggcga
     1561 tccacagcag acgttcctcc tcgaccgaga agaccttgcg gcgcccgtcc attgccgcgg
     1621 caacctcaaa cagggttccg tattccttaa gtccttcgag caggtgccca agttcggtca
     1681 gcaacgccat ttccgcgtcg cttgactcga ggaactgcct gttccggctg atggtgccgt
     1741 cgtcttcgcg ctcgaccggt gaaatggagt taatcaatac gtcgcgggca tcggtttcaa
     1801 agcctgatga tttaagcttt ttgaccttct tcaggatttc atcgaccttg gccttcttcg
     1861 ccgtatattc cgcccaacgc tcatcaaagc gcttacctgc cgcaccaaat gccttcagtt
     1921 tgtcagcggc cttcagtgtc tggtcgccgt agaaggccct gacgacttca accagcgctg
     1981 ccggcacgcc atcgaccaca tccgccgcct tcagatccgt gtcgtacgcc gaattgacgg
     2041 ccttgaccag gcggtcgatt gcccgatcgc ccttctgttc gccggccctt gacggatccg
     2101 ccttcttaac gagctgggca gccgacggcg ccgagcacag cagaatcagc accgccacag
     2161 agtgaaaggc aattcttttc gatgcgttgc gcatgatcgg gaaccccact gcagtaatga
     2221 atgaattctg acgggtaatt atcctttcaa caccgccgcc agtcaacgcc accgaccgtc
     2281 ccccgtgcaa atcgtgccat tttccccgcc gcggcccctg tacgggcgga ttacatccgc
     2341 cggccgcgaa tcgatacttc aaaagtgcaa tcccagttcc gtgccttcca tttgaattcg
     2401 atattgaacc ttcaagcgct tctggttaaa atccggcctt gaccgatatc cgtcatttcc
     2461 agggttgatt catgcatcag tccatttccc gcggttcagt tcccagcctg aaacggattg
     2521 gatacattgt gttcgcgatg tcggcgcttg ccttgaccgg atccgggtgt tccggctcgt
     2581 ccgacgaatg catcgattca atcgattgtc agcgcggtct tgtctgccgg tcgggcgaat
     2641 gcatccttct ggcgtgttca ccggcgggcg gttgcagcga ccttgaggtc tgcacccccg
     2701 gcagttacgg cggaaacgat ccgaacaagg atttctgcag cgctcgttgc agtgtcttca
     2761 acaagtgccg cgccggcgaa cgatgcaccg atggcgtctg cctcccggat gattatgtcg
     2821 atgatattca cgatgtcccg caggatgaag gtagctcggt cgatatgatc ggggattccg
     2881 gtggcgccga ttcgcacgcc ggtgacttgc aggaagacgc gcgggattcc gatgcaccgg
     2941 acgacacacc cgatgaaacc gacatgcccg atgccggtga ttccgggccg gatggcgact
     3001 tgtcccttga cgaagggccc caggattccg ggccgcagga tgttggaacg gacgccgacg
     3061 tcgaaccgga agtccagatg gtctttgtcc ccccggcgac attctggatg ggctgtaacc
     3121 agggcatcga cggtggctgc ggtgaggggg aacttcctta ccacaaggtt gatttgtcgg
     3181 ggtattacat cgacagaacc gaggtcaccc agggcgaata tgatcaatgc gtgcttgccg
     3241 ggaagtgttc acggcccgcg tgtggcttca atccggcttc gacaccggac aaaccggtcg
     3301 tctgcgtcac atggaaccag gccgggatgt actgttcctg ggcgggcaag caactaccga
     3361 cagaggccca gtgggaacat gccgcacgcg gtaccgatgg tcgcgtcttt ccatggggaa
     3421 atgaagcccc aacgtgtgaa ctggccgtca tgtccggatg cggaaccagc atcgggaaag
     3481 tctgcaccag atccccggcg ggggacagtt tccacggcct gtgcgacatg gccggaaatg
     3541 cctgggagtg ggtaaacgac tggtatggcg agcagtacta cgcgacttca gcaagcgtcg
     3601 accccaccgg cccgacttcg ggtcgatccc gcgtggcgcg cggcggctca tttgacaata
     3661 ccgccggccc cttaagagcg tctgcccgaa gctatggaaa agacacgttt tccgcggaaa
     3721 acctgggttt caggtgtgcc ttaagccgcg gcgagcccga aatttgtgac ggcgtcgata
     3781 acgacatcga cggactgacc gacgaagact tcgggatttc ggaatgggac ggaacgattg
     3841 ccggcgttgg acagccgtgc ggaaccggag cgtgtgccgg tggccgcgtg ctttgcaacg
     3901 aagacggcat cggttccatc tgcgacacct cgcacctgat ttccgcggaa attgccaacg
     3961 ggattgacga tgactgtaac ggcttaaccg atgatttgcc ctgtgttcct gactgctcgg
     4021 gtcgggaatg cggattggat ccggtctgcg gaaccatgga ttgcgggccg tgttctggtt
     4081 ccgatgcctg catcgatggc gcttgcgtct gggctcctga ccagtaccag gtgctttgcg
     4141 gcgcggactc gatgtgcttc gtcccggacg gcgttttctg gatgggctgc aatgaaacgg
     4201 tcgatccgga atgtggcgat gatgagcgcc cgtatcacca ggtcgggttg tccgcgtact
     4261 acatagacag aaccgaggta acccaggccg attacgcaaa atgcgtggat gccggggtgt
     4321 gcgcccagcc gacttgcaac tggaatccgg ccgcaaccgg cagcttcccg accgtatgcg
     4381 tcgattggga tgatgccgac accttctgca gatgggccgg caagcgtctg cccactgagg
     4441 ccgaat
//
LOCUS       DAOPPA010000261         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21309_length_4446_cov_3.522888, whole genome shotgun sequence.
ACCESSION   DAOPPA010000261 DAOPPA010000000
VERSION     DAOPPA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21309_length_4446_cov_3.522888"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKH54_08665"
     CDS             complement(<1..529)
                     /locus_tag="PKH54_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00006.1"
                     /translation="MKTVRVAPPVTMTPAVAACLALAMIVFGGGCDRSAPGPDPVPPL
                     PALHAEGTKIVDDAGAEVILRGVNLGGWLFNETWITQIDYPLLSRIHVIAVREGLGDV
                     VDPILTQKSSEWQGSAYLTELESALAAEIGTEAAATFVDKVEPYTPPLYDDSDLLLRR
                     KLSERFGDAARDQLLD"
     gene            643..2199
                     /locus_tag="PKH54_08670"
     CDS             643..2199
                     /locus_tag="PKH54_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOD00007.1"
                     /translation="MLCDFKWLVVCAALISFGGGCGGREADVDAVGDIIEEVAPGDEG
                     NPTDLSTDPGRDGGDQADGGDAEMPVDVADDDGDVVQPKICSVRAHGAVGDGVTLDTA
                     ALQAAIDECAGTGGVTVVEPGIYYTGTIFIKSDMTFRISEGATILGSTAQEDYDGECL
                     VAIRDAENVILEGPGIIDGNGPTWWFWHWIDTSAYRPGKMLQPRNSRNLTFRNLWLTN
                     AAGWHFQLLACDDVLIDNVTVRTLVDETSQSPNTDGIDIDACRNVEVKNCDIETGDDA
                     IVLKNSSGGWARESYNINVHDCTVASWANGLKIGTRPTQPVHDVVFRDIVVQASVHTN
                     PGTRVMGGVTLLADDGADVYNVLAERIHMKAVRSPFFLRVQERILDDEGDRRTEAGRL
                     YNITLKDITVEDCQIPGMIMGIPGFPVENVTIENVSIVSSVAGTESDRDIIPDERNLE
                     YPDAVYFGTMPAYGVYARHVTGPLKFSGEVTFASTPDEKRAAVILDDVAEYDLAGIAG
                     DPEIIVVGQE"
     gene            complement(2239..4134)
                     /locus_tag="PKH54_08675"
     CDS             complement(2239..4134)
                     /locus_tag="PKH54_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOD00008.1"
                     /translation="MTTRDGTKLSYFLTLPGPPENGPYPTVVSYSGYSPSRPGRVLSS
                     EIEPFCEIYPILCNAPDDPSNMIAALIGFATIGVNMRGTGCSDGGYDYFEEVQLLDGY
                     DVIEIVGRQPWVKNNKVGMVGISFPGISQLFVASQRPPHLGAIAPQSVIADSATSCLL
                     PGGIYNDGFVQTWHDMVLYAALPYHHGWVRDVIDAGDELCDRHQKMHLQQRDAIAEGL
                     KYPYYTDEVAMPMDPSAWVDKIQVPVFMTGQWHDEQTGPHFAALMDKFTGSPSAHFTV
                     TNGFHNDSASPQSLIEWMEFLYLFVSKEKVSIPDDVRGLGPMFMEQVFGAQIQFPKSD
                     IEDNADTYEEALAMWNARPPVKVIFETGAAPKTPAAAPAGTFDVRFDEWPLPGTEVAR
                     WYLGPNATLNRQAPDAMGGFSSFAHDAAAGHKVILSSGDVYRNPPNWNWGQPAFGTSV
                     EFLTEPLDQDTVMGGHGSADLWIRTDATDADLEVTVTEVRADGKESYVQAGWLRASHR
                     ALRADATELRPVKSHRFDDVSLLTPGEWTPIRLEIMPFTHIFRAGSRIRVIIDTPGDS
                     MTRWKFRLVEFETPPTIDIGHDADHPSSIALPLVPGVQIPTTQPACTSLRGQPCRDYQ
                     AITPPIQ"
     gene            complement(4225..>4446)
                     /locus_tag="PKH54_08680"
     CDS             complement(4225..>4446)
                     /locus_tag="PKH54_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00009.1"
                     /translation="VGPPYRVSTSFDARPGIEQVHIRDSAPETTLQLIDVNGTLVYEA
                     VSDEYGSLIFRFVPPGRGYSVRLADDASD"
BASE COUNT          869 a         1224 c         1458 g          895 t
ORIGIN      
        1 tgtccagcag ttggtcgcgg gcggcatcgc caaagcgctc tgaaagcttg cggcgcagca
       61 gcaggtctga atcatcgtac agggggggag tgtacggctc gactttgtcg acgaaggttg
      121 ccgcggcctc ggttccgatc tccgcggcca gggcgctctc aagttccgtc aagtaagccg
      181 atccctgcca ctcgctggat ttctgggtaa ggattggatc cacaacatcc ccaaggcctt
      241 cacgaaccgc gataacgtga attcttgaca acaggggata gtcaatctgg gtaatccagg
      301 tctcgttgaa aagccatccg cccaggttca cgccacgaag gatgacttcg gcgccggcgt
      361 catcgacgat ttttgtgccc tcggcgtgaa gggccggcag cggcggaacg gggtcgggtc
      421 ctggcgcgct ccggtcgcat cctccgccga agacgatcat tgcgagggca aggcaggcgg
      481 ccacggcagg cgtcatggtc acgggcgggg ccactcgtac ggttttcatg gtacagacat
      541 cctgtttcag agttttcagt ctggtcgcgg cgcggccgat tgacatcaag ggccgctttt
      601 gaaatagtag caaatggtga cagtttctga aggaattttt gtatgctttg tgatttcaaa
      661 tggttagtcg tttgtgcggc gttgatctcg tttgggggcg gttgcggggg gcgcgaagcg
      721 gatgttgatg cagtcggcga catcatcgaa gaagttgcac ctggcgacga gggcaatccc
      781 actgatttat cgacggatcc cggccgggac ggtggcgacc aggccgacgg aggcgacgcg
      841 gagatgcccg tggatgttgc ggatgacgat ggtgacgtcg tccagccgaa gatatgcagc
      901 gtcagggcac atggggctgt cggcgatggc gtgacactcg acacggcggc ccttcaggct
      961 gccatcgatg aatgtgcggg aaccggcggc gtgactgtcg tcgagcccgg gatctactac
     1021 accggcacca tattcatcaa gagcgacatg acctttcgca tttcagaggg cgcgacgatt
     1081 cttggttcga ccgcccagga ggattacgac ggcgaatgcc tggtggcgat tcgcgacgcg
     1141 gaaaacgtga tccttgaagg ccccggaatc attgacggga acgggccgac atggtggttc
     1201 tggcattgga ttgatacttc ggcttatcgg ccgggcaaga tgcttcagcc gcgcaattca
     1261 aggaacctga ccttcaggaa cctgtggctt acgaacgcgg cgggatggca tttccagttg
     1321 ctggcctgcg acgacgtgct gattgacaac gtgaccgtca ggacattggt cgatgaaacc
     1381 agccagagcc ccaataccga cggcatcgac atcgacgcat gccgaaacgt cgaagtgaag
     1441 aactgtgaca tcgagaccgg cgatgacgcc atcgtgctga agaacagttc cggcgggtgg
     1501 gcccgggagt cgtacaacat caacgtgcat gactgcaccg tagcttcctg ggccaacggc
     1561 ctgaagatcg ggacgcgtcc gacccagccc gtgcatgacg tcgtttttcg cgacatcgtg
     1621 gttcaggcgt ccgtccacac aaaccccggg acgcgcgtga tgggtggcgt gaccttgctg
     1681 gcagacgacg gggcagacgt ctataacgtt ctggccgagc gcatccacat gaaggcggta
     1741 cggtcgccgt tcttcctgcg tgtccaggag cgcatcctgg atgacgaggg ggatcgcaga
     1801 accgaagccg ggcgcctgta caacatcacg ttgaaggaca tcacggttga agattgtcag
     1861 atccctggaa tgatcatggg gattccggga tttccggtcg aaaacgtaac catcgagaat
     1921 gtgtcgattg tttcgtccgt cgcgggaacc gagtcggatc gcgacatcat tcccgacgaa
     1981 aggaacctgg aatacccgga cgccgtctat ttcggaacga tgccggcgta tggcgtttac
     2041 gcccgccacg tcacggggcc gttgaagttt tccggcgagg tgacgtttgc ttcgacgccg
     2101 gatgaaaagc gggcggcggt catcctggac gatgtggccg agtatgacct tgcgggaatt
     2161 gcgggcgatc cggagatcat cgtcgttggt caggaatagc cgcggaattc gcccggaacc
     2221 ctcaattttc accggaattt attgaatcgg gggcgtaatc gcctggtagt cgcggcatgg
     2281 ctggccgcgc agcgatgtac aggcaggctg ggtcgtcggg atctgaacgc ccggaaccag
     2341 cggcagggcg atgctggacg gatggtcggc gtcgtgtccg atgtcgattg ttggcggcgt
     2401 ttcgaattca accaggcgga acttccagcg cgtcatgctg tcgccgggcg tgtcgatgat
     2461 tacccggata cgcgacccgg cccggaaaat gtgtgtaaat ggcatgattt ccagcctgat
     2521 tggcgtccat tcgcccgggg tcagcagcga tacgtcatcg aagcgatggg atttaaccgg
     2581 gcgcaactcg gtcgcgtcgg ccctgagtgc gcgatgtgaa gcacgcagcc agccggcctg
     2641 gacgtagctt tcctttccgt ccgcgcggac ttctgtcacg gtcacttcca gatccgcgtc
     2701 cgtggcgtcg gttctgatcc aaaggtcggc gctgccgtga ccgcccatca ccgtgtcctg
     2761 gtccagaggt tccgtcagga attcaaccga tgttccgaaa gccggctggc cccagttcca
     2821 gttgggggga ttcctgtaaa cgtcgcccga ggacaggatc accttgtgtc cggccgcggc
     2881 gtcgtgcgca aacgaactga acccgcccat ggcgtccggg gcctgcctgt tcagtgtggc
     2941 gttcggcccc aggtaccagc gggcgacctc cgttccggga agcggccatt cgtcaaagcg
     3001 aacatcgaat gtgccggccg gcgctgccgc gggtgtcttc ggggccgccc cggtttcaaa
     3061 gatgaccttg accggaggcc tcgcgttcca catcgccagg gcctcctcgt atgtgtcggc
     3121 gttgtcctcg atgtccgatt tcgggaactg gatctgggca ccgaagacct gttccatgaa
     3181 catcggacca aggccgcgga cgtcgtccgg gatgctgacc ttttcctttg acacgaacag
     3241 gtacaggaat tccatccatt cgataagcga ctgcggggac gcgctgtcgt tatggaagcc
     3301 gttggtcacg gtgaaatgcg cgctgggcga gccggtgaac ttgtccatca gggcggcgaa
     3361 gtgcgggccg gtctgttcat cgtgccattg tccagtcatg aacaccggga cctggatctt
     3421 gtcaacccat gcggaagggt ccatcggcat cgcgacttcg tcggtgtaat acgggtactt
     3481 caggccctcg gcgatggcgt cgcgctgttg aaggtgcatt ttctggtggc ggtcgcagag
     3541 ttcgtcgccg gcgtcgataa cgtcgcgaac ccaaccgtgg tgatagggaa gcgccgcgta
     3601 caggaccatg tcgtgccagg tctggacgaa tccgtcgtta tagatgccgc ccggaagcag
     3661 gcacgatgtg gcggaatcgg caatgacgga ctgcggggcg atggcgccaa ggtgcggcgg
     3721 gcgctgcgat gcgacgaaaa gctggctgat tccggggaac gaaatgccga ccatgccgac
     3781 tttgttgttt ttgacccatg gctgtcgacc gacgatctcg atgacgtcat agccgtcgag
     3841 caactgcact tcttcgaagt agtcgtaacc gccgtcggaa cagccggtgc cgcgcatgtt
     3901 gacgccgatt gtggcaaagc cgataagggc cgcgatcatg tttgacgggt cgtccggcgc
     3961 gttgcacagg attggataga tttcgcagaa cggctcgatt tccgacgaca gtacccgacc
     4021 cgggcgcgaa ggcgaatatc ccgagtagga aaccactgtc ggataggggc cgttttccgg
     4081 cggaccggga agtgtcagaa agtacgacag cttcgtgccg tcgcgggtgg tcaggtaacc
     4141 gaaaccgggt tcaaggatct gatcctggta gaaactgtcc ggcggaaggc tgttttcgac
     4201 agacttgacg tacagcgggc cggtttagtc cgaagcgtca tcggccaggc ggaccgagta
     4261 gccgcgtcct ggcggcacaa agcggaagat aaggctgccg tactcatcgg aaacggcttc
     4321 gtaaaccagc gtgccgttaa catcgatcag ttgcagggtg gtttccgggg ccgagtcgcg
     4381 gatgtgaacc tgttcaatgc cggggcgtgc gtcaaacgac gttgaaacac ggtacggcgg
     4441 gccgac
//
LOCUS       DAOPPA010000262         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21339_length_4440_cov_4.155302, whole genome shotgun sequence.
ACCESSION   DAOPPA010000262 DAOPPA010000000
VERSION     DAOPPA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21339_length_4440_cov_4.155302"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKH54_08685"
     CDS             <1..196
                     /locus_tag="PKH54_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOD00010.1"
                     /translation="AGIDGVYGARLTGAGFGGNTVNLVRQDAAVDFCDRLAVEYRGAT
                     GIESVVRVVRPASGLLSGRF"
     gene            242..1600
                     /locus_tag="PKH54_08690"
     CDS             242..1600
                     /locus_tag="PKH54_08690"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOD00011.1"
                     /translation="MTRDSEFDGAVRAFEGLRAHLEAVPRDCELLQILCEEGFGASIG
                     RWVPMSAPPAVFERLFLDLARVDFAWLRRLRSDVLAAPTDGSGGVPELAPFPYAELSG
                     AGIGDLASRGEADLVSGRWGVVIFAGGAATRFWAGASGHPQARAILSRYGGNAPKGLF
                     PVAPLTGRSFLDLFAEQMLQSGRRHGRMAPLVLMAGRATRDALLEWVETSLPEGFDRS
                     LVKVMVQADHPRLDMDGHLLIRADGSLVFTGDGHGGVFKALLDPEYGDGGVAGWLRGL
                     GVRSIVLHNVDNVAAMALEPARLGFHARGGFSMTMSAVLRVDPLEKVGIIAMNRVTGR
                     VEVVEYSVCPVQTATAVDAAGRLLLVPAHINTNLVELDAVRPDLPPTLYGGKEVDIGG
                     RLVRACSHEMLNQSLAGLLEPSRVGVLVLERDRYFQPTKSLDGVDSLVTTREWLGRRG
                     ID"
     gene            1760..2959
                     /gene="pgk"
                     /locus_tag="PKH54_08695"
     CDS             1760..2959
                     /gene="pgk"
                     /locus_tag="PKH54_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD00012.1"
                     /translation="MAGFRDMDRLEDMSLDGRRVLVRVDMDCPVDEDGTIIDDSRIQL
                     CLPTIRHLITEGARVVLATTLGGQMGRRNPKLSLALLGERLAEILDRDIFLPEDTVGE
                     GPWKVIMERVEGEVVLLENLGFCPEDDRNDDLFSQRLFSLADVFVNEAFRLSDRVLAS
                     TVGVSALFNEKVAGLRFRREVEALSRMVQAPEGQGFIFGGPDDGMALEFLANHPGRAR
                     KVFLAGHAALPFYVAKGWRTGSHKVEKDRVEQAGKVLSRAQLRGVEIVLPEDFLVVPD
                     GAGHDDARIVRSDGVPGDHVILDVGPATVEVFCGGLRGLRSLVWHGALGTCRGPDCTA
                     SSDKVARSVIRTSAAATIIGDEMAEVVSRLVLTPFFSFVSCGGDIGMKMLLGRELPGV
                     KALCGGS"
     gene            2959..3723
                     /gene="tpiA"
                     /locus_tag="PKH54_08700"
     CDS             2959..3723
                     /gene="tpiA"
                     /locus_tag="PKH54_08700"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD00013.1"
                     /translation="MVSRKPLVAGNWKMNLDVEGARTLAREVLDRVSRFRGVEVAVCP
                     PFVLIPEVVRVMEGSAVGVGGQDVSTEVKGAFTGEISGDQLVSVGCRYAIVGHSERRQ
                     YHFESDELISAKVKRALAVGLTPIFCVGERKDERVAGRTFERVERQVTVGLAGLDAAA
                     MASVVVAYEPVWAIGTGDVATPAQAQEVHEFIRGLLVRMFGQETAAATRILYGGSVTA
                     ENAGELMGQSDVDGALVGGASLKSASFGAIVEAAGV"
     gene            3726..4127
                     /gene="secG"
                     /locus_tag="PKH54_08705"
     CDS             3726..4127
                     /gene="secG"
                     /locus_tag="PKH54_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD00014.1"
                     /translation="MGILYGLLLASFIVVALLLIVIILIQPGKSGGMGAAFGGGSSSS
                     VFGGRGATTFLTKATGILAGLFFVLSILIALTNVEPSVTRDIPATKPAVGGTAAPAPA
                     PVAAQPSAVDPAAPAPEGAPAPAAAPAGEGL"
     gene            4168..>4440
                     /locus_tag="PKH54_08710"
     CDS             4168..>4440
                     /locus_tag="PKH54_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00015.1"
                     /translation="MKHEKRSPVIIAVANQKGGVGKTTTSVNLAASLAVYEVPTLLID
                     MDPQANASLAFGIDYRAGGLHVYDVMLGRAGLSEAVRHTELGSLDVL"
BASE COUNT          685 a         1245 c         1533 g          977 t
ORIGIN      
        1 ggccggcatc gacggtgtgt acggcgcccg cctgaccggc gccggattcg gcgggaatac
       61 ggtcaacctg gtgcgccagg atgcggcggt cgatttctgc gatcggcttg cggtcgaata
      121 caggggcgcg accgggatcg agtcggtggt cagggtggtt cgtccggcca gcgggctgct
      181 ttccggtcgg ttctgacggc ggaaatcggt tggatcgacc atttatcagg cgggtatcca
      241 gatgacacga gattcggaat tcgatggcgc tgtgagggcg tttgaggggc tgcgggccca
      301 ccttgaagcg gttccgcgtg attgcgagct gcttcaaatc ctttgtgagg aaggtttcgg
      361 tgcctcgatt ggacgatggg ttcccatgtc ggccccgccg gcagttttcg aaagactgtt
      421 tctggatctt gccagggtcg attttgcctg gttgaggcgg ttgcgaagcg atgtccttgc
      481 cgccccgacc gatggctccg gtggcgttcc ggaactggcg ccgtttccgt acgcggaact
      541 gtcaggggcg gggatcgggg atctggcttc gcgcggcgag gccgatcttg tctcggggcg
      601 atggggcgtc gtcatctttg cgggcggggc ggccaccagg ttctgggccg gagcgtcggg
      661 acatccgcag gccagggcca tcctgtcgag gtatggcggg aacgctccca aggggctgtt
      721 tcccgtggcg ccgctcaccg ggcgatcctt tcttgacctg ttcgcggagc agatgctgca
      781 atccggacgc cggcatgggc gaatggcccc actggtgctg atggccggcc gggcgacccg
      841 ggacgcgctt ctggaatggg tcgagacatc gctgccggaa ggttttgaca ggagtctggt
      901 gaaggttatg gttcaggccg accatcccag gctggacatg gacggccatc ttcttatcag
      961 ggcggacggt tcccttgttt tcaccgggga tggccacggt ggcgtgttca aggctctcct
     1021 tgatccggaa tacggcgatg gcggggttgc cggctggctt cgggggcttg gcgtccgcag
     1081 catcgtgctt cacaatgtcg ataacgtcgc tgccatggcg cttgagcccg cgcggctcgg
     1141 ttttcatgcc cggggcgggt tcagcatgac gatgtctgcc gttttgcggg tggatccgct
     1201 tgagaaggtc ggcatcatcg ccatgaaccg ggtcaccggt cgggttgagg tggtagaata
     1261 ctcggtgtgc ccggttcaga ccgcgacggc tgtcgatgcg gccggaagac ttctgctcgt
     1321 tcccgcccac atcaatacga atctggtcga gctggacgcc gtcagacccg acctgccgcc
     1381 gaccctttac ggagggaagg aggtcgatat cgggggacgg cttgtgaggg cctgctccca
     1441 cgagatgctc aaccagtcgc ttgccggcct cctggaacct tcccgggtcg gcgtgctggt
     1501 tctggagcgt gatcgatatt tccagcccac taagagtctt gacggagtcg attcccttgt
     1561 gaccacaagg gagtggcttg gccggcgcgg gatcgattga cgggcgccgt cctccggcag
     1621 atcggcttcc ggacggtccg gcgccgaggt ttgtcgcggt ttttattcgg gatttcaccg
     1681 ggatccttgc gattgacacc ggacgtcttt ctgataaagt atgcgaccgg ccgaatacgg
     1741 ccagggtttt gaggttgcgg tggctggctt ccgtgatatg gacaggctcg aggacatgtc
     1801 cctggacggc aggagagtgc tggttcgggt tgacatggat tgccctgtcg atgaagacgg
     1861 caccatcatc gacgattccc gaatccagct ttgtcttccg acaatcaggc acttgatcac
     1921 cgagggcgcg agggtggtgc ttgccaccac gctcggtggc cagatgggcc gccgcaatcc
     1981 gaagctttcg cttgcgcttc ttggcgagag gcttgccgag attcttgacc gggacatctt
     2041 cctgcccgag gacaccgttg gcgaaggccc gtggaaggtc atcatggagc gggtcgaggg
     2101 cgaggtcgtc ctgctggaga atcttggctt ctgtcctgaa gatgaccgaa acgacgacct
     2161 tttttcgcaa aggctcttca gtctggccga tgtattcgtg aacgaggcat tcaggctttc
     2221 ggatcgggtg cttgcgtcaa cggtcggggt ttccgcgctc ttcaacgaga aggttgccgg
     2281 actccggttc cgtcgggagg tcgaggcgct ttccaggatg gttcaggcac cggaaggtca
     2341 ggggttcata ttcggcggtc ccgacgacgg gatggccctt gagttcctgg cgaatcatcc
     2401 cggtcgcgcc aggaaggttt ttctggccgg tcacgccgcg cttcccttct acgttgcgaa
     2461 gggttggcgt accggttccc ataaggttga aaaggatcgc gtcgaacagg cgggcaaggt
     2521 tctttccagg gcccagcttc gaggcgttga aatcgttctt cccgaagatt ttctggtggt
     2581 tccggacggc gcggggcacg acgacgcacg tatcgtgcgc tccgacggcg tccccggcga
     2641 tcacgtgatt ctggacgtgg gacccgccac ggtcgaggtg ttctgcggcg gcttgagggg
     2701 tctgcgttcg ctggtgtggc acggggcgct cggaacctgt cgggggcccg attgcacggc
     2761 atcttcggac aaggtcgccc gaagcgtcat ccgcacctcg gcggcagcca cgattatcgg
     2821 tgacgagatg gctgaagtcg tgtcgcgtct tgtcctgacc ccgttctttt cattcgtgtc
     2881 ctgtggcggc gatatcggta tgaagatgct gctcggcagg gagctgccgg gggtcaaggc
     2941 gctttgtgga gggtcctgat ggtttcgaga aagcctcttg tggccggcaa ctggaagatg
     3001 aatcttgacg tggaaggcgc cagaaccctg gcgcgggagg tccttgacag ggtttccagg
     3061 ttccgcggcg tggaggttgc ggtttgcccg cctttcgtgt tgattcccga ggtggtgcgc
     3121 gtgatggaag gctccgcagt cggggtcggc ggccaggacg tcagtaccga ggtcaagggc
     3181 gcttttaccg gtgagatcag cggggatcag cttgtcagcg tcggttgccg ctatgctatc
     3241 gtcggtcatt cggaacggcg ccagtatcac ttcgaatcgg acgagctgat ctcggccaag
     3301 gtcaagcgcg cgcttgcggt cgggctgacg ccgattttct gtgtcggtga acgcaaggac
     3361 gagcgcgttg ccggtcgcac ttttgagcgc gtggagcggc aggtcaccgt cggactggcc
     3421 ggtctggacg ccgcggccat ggcttccgtg gttgttgcat atgagcccgt ctgggccatc
     3481 ggcaccggcg atgtcgccac gccggcccag gctcaggagg ttcacgagtt catccgcggc
     3541 cttcttgtca gaatgttcgg acaggaaacc gcggcggcga ccaggattct ttacggcggc
     3601 agcgttacgg cggaaaatgc cggcgagctc atgggccaga gtgatgttga tggcgcgctt
     3661 gttggcgggg ccagcttgaa gtcggcttct ttcggcgcaa tcgtcgaagc cgcgggggtt
     3721 taatcatggg tattctttac ggtctgcttc ttgcttcgtt catcgttgtc gcgcttctgc
     3781 tgattgtgat catcctcatt cagcccggaa aaagcggcgg aatgggcgct gcgttcggtg
     3841 gcgggtcatc gtcgtcggtc ttcggcggtc gtggcgcgac gacgttcctg acgaaggcca
     3901 ccggaatcct ggccggcctg ttcttcgtgc tttccatcct gatagctctg accaatgtcg
     3961 agcccagcgt gacccgcgat attccggcga ccaagccggc agtcggcgga accgctgctc
     4021 cggccccggc ccccgtggcc gcacagcctt ccgcggtcga tcccgccgcc ccggcgcctg
     4081 aaggcgcccc ggccccggct gctgctccgg ccggcgaagg tctctgattc atgcgttccg
     4141 aactggactt tgtccgggtt gtttcacgtg aaacacgaaa aacggtcacc tgtcataatc
     4201 gcagtcgcaa atcagaaggg cggcgtcggc aagacgacga cgtcggtcaa tcttgccgca
     4261 tccctggcgg tgtatgaagt tccgaccttg cttatcgaca tggatccaca ggccaatgcc
     4321 agcctggcat tcgggattga ctaccgggcc ggcggacttc acgtgtatga cgtgatgctc
     4381 gggcgggccg ggctttccga agccgtcagg cacaccgagc ttgggtcgct tgacgtgctg
//
LOCUS       DAOPPA010000263         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21392_length_4433_cov_4.068981, whole genome shotgun sequence.
ACCESSION   DAOPPA010000263 DAOPPA010000000
VERSION     DAOPPA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21392_length_4433_cov_4.068981"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKH54_08715"
     CDS             complement(<1..208)
                     /locus_tag="PKH54_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00016.1"
                     /translation="MKQAIHLLLATLAVACGMVSCAEEDSRPDFGPCVPNTCVSRGHV
                     CGTIEDGCGALISCGTCPDGQVCNH"
     gene            complement(221..1081)
                     /locus_tag="PKH54_08720"
     CDS             complement(221..1081)
                     /locus_tag="PKH54_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00017.1"
                     /translation="MRRSWLLGTGCVVAVMLTGLGQGCDGCGCRKPAEVAVATPEKAP
                     EHIPGLPVPDPEPVQIGPDSPDADLATHFGISADRIAELKKAHAALPGISRPAGLVVT
                     PAKLKPVPTDQERAKEEAIPAEAEPVLSGPERVTGLPALKVILGHEFKAAKTIDPAEL
                     AAESVRLRRLLFNRKLPIFADPILFIDSTLESAQSEVNVTIGIPVPEGVDVPPGIKVR
                     EVRAGKCLFASNMRIDEVAVTEKDWESVTSPSLISLIGAPCKVSLIVDLAGWPEPDRQ
                     SRGNAYIFCM"
     gene            1370..2785
                     /locus_tag="PKH54_08725"
     CDS             1370..2785
                     /locus_tag="PKH54_08725"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOD00018.1"
                     /translation="MRIEWITALIPLVVAGCQDPRLPPWMTDLDHPDVHGEVVVSDSF
                     IDSEEDAGGERDNGHDGDVSDEVAPPDDTAGDLPDDLAVVDSEEDTASDVPDVVVIPD
                     PVCAAPVGECLVSFWDPVKMECVTDSAPDGTPCEDGYCSGGSCSSDCWKGSCPAGLGP
                     ASSCRCVVPSSGSRLCLSEGGFVDCEELRPGYPWSGQDAQLVSGARSFVDNGDGTVTD
                     QVTGLEWERDAFTPLSMELAIEHCFYHFDLPGDGWRLPTIRELFSLIDFGQTTCMWDP
                     VFGDSCSGDRMFWSSTQAYMTANVCTLHPRGNIEACELDGLNRVRCVRGDLPPPDPVP
                     DRYLAFEQVVLDRATGLGWQNVSSFQPMEWAEGLRACQRLGTGWRLPTSDELVSIIDM
                     TMHIGKAYSETGDPCAKWDDALGRFCSQQLYFWTSTPNPRLTDPVSAYAVHFFSGHVH
                     ENPVQGSRSQKFNVRCVKDFR"
     gene            2879..3784
                     /locus_tag="PKH54_08730"
     CDS             2879..3784
                     /locus_tag="PKH54_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00019.1"
                     /translation="MLRVVLITLVAIGFTGCELFDVIGNIGGPGTCDVFSENDCTECG
                     SSCSDAIDNDAEIGTDAVCNDGNAGEVATADIVQADESAGVDAVVKADPLTGFEIRKP
                     LPRQIDPGLTVEDVDYVCTLVYGEIDAYFYIQTTPFKCSATGLCQFQTIGAWISDGGL
                     VRPVEASYNWGGHHHNDWILLRLKGLTVRYNHSSYGFGWRSCQAPDCLQIETDGMLDK
                     DGCEPERTLPVVCRQVWADGTYDPLVDDFRTCPGDVSSDPPGGADDDDDDQGDDSPDD
                     DDDHGDDDSRDQDRDEDHERRDDGD"
     gene            3901..4323
                     /locus_tag="PKH54_08735"
     CDS             3901..4323
                     /locus_tag="PKH54_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00020.1"
                     /translation="MKSACEVPPSIKPVFQKNPDFLMADSQKNLDFLKPRLLREVWRH
                     SFVGGMPEAVASYRKTGKMVDAFAVHAELGRSFRDDFAKYTPRVNPLAIVQRPDRAGQ
                     DPLDPPRAAMRDSRRRFHRSSSRERVDDVSIYSPAAAG"
BASE COUNT          858 a         1270 c         1328 g          977 t
ORIGIN      
        1 catgattgca gacctgtccg tccgggcatg tcccgcagga aataagagcc ccgcacccgt
       61 cctcgatggt tccgcaaaca tgaccgcggc tcacgcaggt attcggcacg cagggtccga
      121 agtccggccg ggagtcctct tcggcgcacg ataccatgcc gcaggcaacg gccagcgtcg
      181 ccagcagtag atggatggct tgcttcatca aaaaaacctt ttacatgcag aatatgtagg
      241 cattgccgcg ggattgccgg tctggttctg gccatccggc aagatcgacg atgagggata
      301 ccttgcaggg cgccccgatc agtgaaatca gggatggtga agttacggac tcccagtctt
      361 tttcagttac ggcaacttca tcgatcctca tgttgcttgc gaaaaggcac tttccagccc
      421 ggacttcgcg aaccttgatt ccgggcggaa catcgacccc ttccggaacc ggtatgccga
      481 tggtcacatt gacttccgac tgggcgcttt ccagggtcga atcgatgaac aggatcggat
      541 cggcaaagat cgggagcttc ctgttgaaca aaagtctgcg caatctgacc gattccgctg
      601 ccagttcggc gggatcgatt gtttttgccg ccttgaattc gtggcccagg atcaccttca
      661 gcgccggcaa gcctgtgacg cgttccgggc ccgaaagaac cggttcagct tcggccggga
      721 tcgcctcttc ctttgcccgc tcctgatcgg tcggaaccgg cttaagcttg gccggcgtga
      781 ccacaagacc ggccggtcgg ctgatccctg gcagtgccgc gtgtgccttc ttgagttccg
      841 caatcctgtc cgcgctgatt ccaaaatgcg tggcaagatc cgcgtcgggc gaatcggggc
      901 cgatctggac tggttcggga tccggaaccg gaaggccggg gatatgctcc ggtgcctttt
      961 ccggcgtcgc gacggcgact tcagcaggtt tgcggcaccc gcacccgtcg cacccctgac
     1021 caaggcctgt caacatcaca gccacaacgc atcctgtccc cagcaaccag cttctgcgca
     1081 tttgatttcc cccgacaagc ctggaagtca ggggcattat cacacggaaa caggtgtcgg
     1141 atccatacgg tctgaattcg gggtcgagcc cagggcggtt cccgcgcatc gcccatgccg
     1201 gcacgatggc gcgcatcagg ccagatttac aattcatttc aaagatttct gttgccttga
     1261 tcaaggtctc ttccatacaa tccttcggta cgaaggcctc ccgggtcttc cgatctgttc
     1321 gtggttcttc gtgctgccgc agtaatcttg cgatatgtgg aattgaatta tgcggattga
     1381 atggattacg gcgctgattc cccttgttgt ggcaggttgc caggatccgc ggcttcctcc
     1441 gtggatgacc gaccttgacc atcccgatgt ccatggtgaa gtcgtcgtgt ccgattcgtt
     1501 catcgattcg gaagaagatg ccggtggcga gcgtgacaat gggcacgacg gcgatgtttc
     1561 ggacgaagtc gcgccgcccg atgatacggc tggcgacctg ccggacgacc tggcggttgt
     1621 cgattcggaa gaagatacgg cgtccgatgt gcccgatgtc gtcgtgattc ccgatccggt
     1681 ctgtgcggcc cccgtcgggg aatgcctggt ttccttctgg gatccggtga aaatggaatg
     1741 cgtcacggat tccgcgcccg acggaacccc ttgtgaagac gggtattgtt caggcggttc
     1801 gtgcagttcg gactgctgga aaggatcatg cccggctgga cttggccccg cgtcgtcgtg
     1861 ccgctgcgtc gttccttctt ccgggtccag gctgtgcctt tccgaaggcg gattcgtgga
     1921 ttgtgaagaa ctgcggcctg gatacccctg gtcggggcag gacgctcaac ttgtttccgg
     1981 cgcgcggtct tttgtcgata acggcgatgg aacggtcacc gaccaggtaa ccggcctgga
     2041 atgggaaagg gatgccttca cgccgttaag catggaactg gcgattgaac attgttttta
     2101 tcacttcgat ctgcccggcg acggttggcg gctgccaacc atcagggagc ttttctcgct
     2161 gatcgatttc ggccagacga cctgcatgtg ggatcccgtt tttggcgata gttgttccgg
     2221 ggatcgcatg ttctggtctt ccacccaggc gtatatgacg gccaacgtct gcacgctgca
     2281 tccccgcggc aatatcgagg cctgcgagct ggacggtttg aaccgggttc gttgcgtccg
     2341 tggggatttg ccgccaccgg atcccgtgcc cgaccgttac ctggcctttg agcaggtcgt
     2401 gctcgaccgc gcgaccgggc tgggctggca gaacgtttct tcatttcagc cgatggaatg
     2461 ggccgagggg ttgcgggcat gccagcgtct tggaaccgga tggcgcctgc cgacatccga
     2521 cgaactggta tccatcatcg acatgacgat gcacatcggc aaggcgtatt cggaaaccgg
     2581 tgacccctgt gccaagtggg acgatgcgct gggacggttc tgcagccagc agttgtactt
     2641 ctggacatcg accccgaatc cgcgcctgac cgatccggtt tccgcatatg cggttcattt
     2701 cttttccgga cacgtgcatg aaaacccggt tcaaggatcc cgcagtcaga agttcaacgt
     2761 tcggtgcgta aaggattttc gttgaaaatc gcccgtttcg ggcggttttt tcatacagta
     2821 ttcaatcggc gagttccttg acggaattcg ggccggttgt ttttaaggtg gcggcgatat
     2881 gttgcgggtc gttttgatca ccctggttgc gatcgggttc acaggctgtg aactttttga
     2941 cgtgatcggc aacatcggtg ggcccggaac ttgcgacgtg ttttcggaaa acgactgcac
     3001 ggaatgcgga tcttcatgtt ccgatgccat cgataacgac gcggaaatcg gcaccgatgc
     3061 tgtttgtaac gacggaaatg cgggtgaagt tgccaccgct gacatcgtcc aggccgatga
     3121 atcagccggc gttgatgccg tcgtcaaggc ggaccccctt accggtttcg aaatcaggaa
     3181 gcccttgcct cgccagatcg acccgggctt gaccgtcgaa gacgtcgatt acgtatgcac
     3241 cctggtttac ggggaaatcg acgcatactt ctacatccag acgacaccgt tcaagtgcag
     3301 tgccaccggc ctgtgccagt tccagacgat tggtgcctgg atatccgatg gcgggcttgt
     3361 ccggccggtc gaggccagct acaactgggg cggccaccat cataacgact ggatactgct
     3421 gcgcttgaaa ggactgaccg tcaggtacaa ccactcctca tacggtttcg gctggcgttc
     3481 gtgtcaggcg cccgactgcc tgcagatcga aacggatgga atgcttgata aggacggctg
     3541 cgaaccggaa aggactctgc ctgtcgtctg ccgccaggtc tgggccgacg gaacctacga
     3601 cccgcttgtc gatgatttca ggacatgccc tggcgacgtc tcgtccgacc ctccaggcgg
     3661 tgcggacgac gacgatgatg atcagggcga cgattccccg gacgacgatg acgatcatgg
     3721 cgacgacgac agtcgtgacc aggaccggga cgaagaccac gaacgccgtg acgatggcga
     3781 ctgattccgt ttttctggca aacagcgcgt tgtctgctgt accgaaccag ccgcggcaac
     3841 gcccaatttg aaaggcccag cccgtcaccc gatcatttcc gctcgcaggt gaagtcgccg
     3901 gtgaagagcg cttgcgaggt acccccttcc ataaaaccgg tatttcaaaa gaaccctgat
     3961 tttctaatgg cagatagtca aaagaacctt gattttctaa aaccacgtct tttgcgcgaa
     4021 gtctggcggc acagctttgt tggtggcatg ccggaagcgg ttgccagtta tcgtaaaacc
     4081 ggaaagatgg tggatgcatt tgcggtgcac gccgaactgg ggcgttcgtt cagggatgac
     4141 tttgcgaagt acacaccgcg ggtgaacccc ttagccatcg ttcagcgccc ggatcgggct
     4201 ggccaggacc cactggatcc gccacgggca gcgatgcggg acagccgtcg tcgattccat
     4261 cggtcttcct cccgggaacg ggttgacgat gtgagcatct actcgcctgc tgccgcagga
     4321 tagggcgtca acatcacgcg attcgcgagc gcgacgcgct gacatggtca aggcgtcggg
     4381 atggcgaatt cagccacgac cgggaaatgg tccgacgtca tcaggccaag gta
//
LOCUS       DAOPPA010000264         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21405_length_4431_cov_4.286106, whole genome shotgun sequence.
ACCESSION   DAOPPA010000264 DAOPPA010000000
VERSION     DAOPPA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21405_length_4431_cov_4.286106"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PKH54_08740"
     CDS             complement(<1..1879)
                     /locus_tag="PKH54_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD00021.1"
                     /translation="MMKYILIAMTAVTALGQAAVAFPAIQRKPESPAFTIRTSAPARV
                     PVTPNVIRLAGNEVSLGHIPHPFGVDARQASSELDAFMASSRDPLPWSAGILPTAFDL
                     RDFPGRVPPIRDQGNCGSCWTFATTGCNEMMLTPAQSWDFSENNMKDRHGFDWGPCDG
                     GNVFMSSAYLARHDGPVQESSDPYKDWDDTADPGWTPVTGPIPIKYYELRLLAADGSN
                     SYVQSIKQAIMDYGPVEVNFRVAVDELGNTNLDAWSANLDAFYSGETDYSNHAVIAVG
                     WDDNYPVSNFNPAIQPPGPGAILIRNSWGDIHNGDGYFWISYHQMIETPAVHMPDVID
                     GTTTPAVHHVYQYDPLGFVAPWGNDTPGVSWTMGNMFKATSRQNLEAASAWMAADGSV
                     QVRVGTGCGATPDSCAFNATVYSRAYDLPGLYAIDLSALGIVLEQDENFFIEYTIGSS
                     TSGYMIPTEMYYADYASGASAQPGESFFYDSGTWIGLQDALGDSSVNLNIKAFTSDVM
                     TLGGLVINEIDYDQPSTDTAEFIEIANRSGATVSLEGYKLELINGSGELLYQTINLPV
                     VDLEAGDYFVICQNPANTPNCDMTFSALAAQNGAPDGLRLVKDGIVIDSMGYEGNMST
                     TE"
     gene            2026..2487
                     /locus_tag="PKH54_08745"
     CDS             2026..2487
                     /locus_tag="PKH54_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOD00022.1"
                     /translation="MAVCGRCGVAGRFLLVLAIAAIGLGTAGCRKPKEPANPKEVKKK
                     LEKVVKLVLWKVDATAEQKNSVDGILDGLSIDLFDIQGENRVLVRKAMDALDAETVDS
                     VALEQVAADGARLFERYLRRMIRAFSDMAGVLAAEQRHRVVKLWREYEFGK"
     gene            complement(2537..4087)
                     /locus_tag="PKH54_08750"
     CDS             complement(2537..4087)
                     /locus_tag="PKH54_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00023.1"
                     /translation="MRRLIHAVVVMAMLGVGCGSAATDSDIIADAADTAAGTDLEAPD
                     LAADDGSTPDLPDSGWLSDAADTGEIDANDDGTPDAAADLPDELSGDGETATDESGDS
                     PGDAGDTTDAPDEGPSDTYVTPDIPEHGELQGKGFFTVQAHEGRWWFVTPDGLPFYSI
                     GINACNPNGSVDASTGENRYKAAVENNYPGETWAQKADSWAAATADRLKSWGFNTVGA
                     WSDWNRLGKTMPYTVILNISGVDWQVGDIPDYFSQEFADRCAQVARDTVAPRRDDPNL
                     IGWFLDNELRWGWDWRSPKTLLQDYLAMPESTPGHAVALEYQGRPYEFLAVVAAKYFQ
                     VTTEAVRSADPNHLILGIRSISVMTHPQVPQAAGPWVDVYSVNNYEFLPELYDAIDAA
                     EQDFLDEGNWLLNYYGITGRPIMITEFSMRSSEADVPSTWPVFYPTYPTQAERAAAYQ
                     AYTENCFAKPYIIGQHWFQWADEPAGGRFDGENSNFGLVDGDDKPYTKLVETMTLIHA
                     QAPDRAPE"
     gene            complement(4124..>4431)
                     /locus_tag="PKH54_08755"
     CDS             complement(4124..>4431)
                     /locus_tag="PKH54_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00024.1"
                     /translation="VDIPATVACPVIVEPIPDATADVIDDSGAVGDTTERDTIGPDQA
                     TSDSTVTDTGVETDDGGTETKGGGCSAGGANGTGAAGFALLFLMLAALAVSRRAFAR"
BASE COUNT          849 a         1270 c         1382 g          930 t
ORIGIN      
        1 cctcggtggt tgacatgttg ccttcgtagc ccatcgaatc gatgacaatc ccatccttta
       61 cgagcctcaa tccgtcagga gcgccgttct gggccgcaag ggctgagaat gtcatgtcgc
      121 agttcggcgt gttggcaggg ttctggcaga tgacgaagta gtccccggcc tccagatcca
      181 cgaccggcag gttgatcgtc tggtaaagca gttcgccgga gccgttaatc aattccagct
      241 tgtacccttc aagcgagacc gtggcgccgg atctgttggc gatttcgata aattccgccg
      301 tgtcggtact gggctggtcg taatcgatct cgttgattac aagcccgccc aacgtcatca
      361 cgtccgaggt aaacgccttt atgttcaggt tgacagatga atcgccgagg gcgtcctgca
      421 gtccgatcca ggttccggag tcatagaaga atgactcgcc aggttgcgcc gaagcgcccg
      481 atgcgtagtc ggcatagtac atctcggtcg ggatcatgta tccgctggtc gaactcccaa
      541 tcgtgtattc gatgaagaag ttttcgtcct gttcaaggac aatacccagt gccgaaaggt
      601 caatcgcgta aaggccggga aggtcatacg cgcgggaata caccgttgcg ttgaaagcac
      661 aggaatccgg cgtcgcaccg cacccggtgc caacccttac ctgcaccgac ccgtccgccg
      721 ccatccaggc cgaagcggct tcaaggttct gccttgaagt ggccttgaac atgttgccca
      781 tggtccagga gactcccggc gtgtcgtttc cccacggggc gacaaatcca agcggatcgt
      841 attgataaac gtgatggacg gccggtgtgg tcgttccgtc gatgacatcg ggcatgtgaa
      901 cggccggcgt ctcaatcatc tggtggtagg aaatccagaa gtatccgtcg ccgttgtgga
      961 tgtcgcccca gctgttgcga atcaggattg ctcccggtcc cgggggctgt atggccgggt
     1021 tgaagtttga aaccggatag ttgtcgtccc agccgacggc gattacggcg tggttcgagt
     1081 agtctgtctc accgctgtaa aaggcatcca ggtttgccga ccaggcatcc aggttggtgt
     1141 tgccaagctc atcgacggcc acccggaagt taacctcgac agggccgtag tccatgattg
     1201 cctgcttaat cgactggacg tacgagttgc tgccgtcggc cgccagaagt ctgagttcgt
     1261 aatacttgat cggtatcggc ccggtcaccg gcgtccatcc cgggtcggca gtgtcgtccc
     1321 aatccttgta cgggtcgctt gattcctgca ccgggccgtc gtggcgggcc aggtatgccg
     1381 acgacatgaa gacgttgccg ccgtcgcagg gaccccagtc gaaaccgtgc ctgtccttca
     1441 tgttgttttc ggagaagtcc cacgactggg ctggtgtcag catcatctcg ttgcagccgg
     1501 tcgtggcgaa tgtccagcac gaaccgcaat tgccctgatc ccgaatcgga ggaacccgcc
     1561 ccggaaagtc gcgcaggtca aaggctgtcg gcagtatccc cgccgaccag ggaagcggat
     1621 ccctggagct tgccatgaag gcgtccagtt cggacgaggc ctgcctcgca tcgaccccga
     1681 acgggtgggg aatatgaccc agcgaaacct cgttaccagc cagccggatc acattcggtg
     1741 tcacgggcac ccttgccggt gccgatgtac gaatcgtgaa ggccgggctt tccggtttgc
     1801 gctggattgc cgggaaggcc acggccgcct ggccaagagc cgtgacggct gtcatggcga
     1861 taaggatgta cttcatcatg gttaatgctc cggggaaaag aatgaacgac ggattcaggc
     1921 gcggaatcta catcaaaatc agtcaattcg caatcgtttc agtggatgac agggcgcatt
     1981 gaaaggtata tgaaaaggaa ttgtaaagtc gatggagggc tgagcatggc agtctgtggt
     2041 cgctgtgggg tcgcaggaag atttctgctg gttcttgcaa ttgccgctat cggtctgggc
     2101 acggcaggtt gccgcaagcc gaaggaaccg gcgaatccga aggaagttaa gaagaagctg
     2161 gaaaaggtcg tgaaactggt cttgtggaag gtcgatgcga ccgccgagca gaagaattcg
     2221 gtcgatggca tcctggacgg tctgtccatc gatcttttcg atattcaggg cgaaaaccgt
     2281 gtcctggtca ggaaggcaat ggacgcttta gacgccgaaa cggtggattc agttgccctg
     2341 gaacaggtgg cggcggatgg ggcccggctt tttgaacgct atctgcgcag gatgattcgg
     2401 gccttttccg acatggccgg ggtgcttgcc gccgaacagc gtcatcgagt cgtcaagctc
     2461 tggcgggaat acgagttcgg caagtagagc gcccgccagg gttcggttcg cccgggccac
     2521 ggatctcgca tgggattcat tcgggggccc ggtcgggcgc ctgggcatga attaacgtca
     2581 tcgtttcaac cagcttggtg taaggcttgt cgtcgccatc gaccagcccg aagttcgagt
     2641 tttcaccgtc gaagcgtccg ccggccggtt cgtctgccca ttggaaccag tgctgcccga
     2701 tgatgtatgg cttggcgaag cagttttcgg tgtaagcctg gtaggccgcg gcacgctccg
     2761 cctgcgtcgg atatgtcgga tagaagacgg gccatgtact gggcacgtcg gcctcgctcg
     2821 aacgcatcga gaattccgtt atcatgattg gccgcccggt gatcccgtaa tagttcagca
     2881 gccagttgcc ttcgtcgagg aagtcctgtt ccgcggcgtc gatcgcgtcg taaagctcgg
     2941 gcagaaactc gtagttattg accgaataga cgtcaaccca tggcccggcc gcctggggca
     3001 cctgcggatg cgtcatcacg ctgattgacc ttattcccag gatcaggtgg tttggatcgg
     3061 ccgaccgtac ggcctcggtc gtaacctgga agtacttcgc ggcgacaacg gccaggaatt
     3121 cgtaaggtct tccctggtat tcaagcgcga ctgcgtggcc gggcgtcgat tccggcatgg
     3181 caaggtaatc ctgaagcagg gttttcggcg atctccagtc ccagccccat cgaagctcgt
     3241 tgtcaaggaa ccagccgatc aggttggggt catcccgtct gggcgccacg gtgtccctgg
     3301 cgacctgcgc gcaccgatcc gcgaattcct gcgagaagta gtcgggaatg tccccgacct
     3361 gccaatcgac gccggagatg ttcaggatga ccgtgtaggg catcgtcttg ccaaggcggt
     3421 tccagtcgga ccacgcgcca acggtgttga aaccccagga cttcaacctg tcggcggtcg
     3481 ccgcggccca tgaatcggcc ttctgcgccc aggtttcgcc gggataattg ttttcgacgg
     3541 cggccttgta ccggttctca ccggtgcttg cgtcaactga accgttcggg ttgcatgcgt
     3601 taataccgat cgaataaaac ggcaggccgt ccggcgtcac gaaccaccag cgcccctcat
     3661 gggcttgaac ggtgaagaat cccttcccct gcagttcgcc atgttccggg atgtcgggcg
     3721 tcacataggt gtcggagggt ccttcgtcgg gggcatccgt cgtgtctccg gcgtccccgg
     3781 gcgagtcgcc ggattcgtca gtcgcggtct cgccgtcgcc ggaaagttcg tccggcaggt
     3841 cggcggcggc atctggcgtg ccatcgtcat tggcgtcgat ttcgcccgta tccgcggcat
     3901 ccgaaagcca gccggaatcg ggcagatcgg gtgtggaacc gtcatcggca gccaggtcgg
     3961 gcgcttccag atcggttccg gccgcggtat ccgccgcgtc ggcaatgatg tcggaatccg
     4021 tggccgcgga tccgcagccg acgccaagca tcgccatcac aacaacggca tgaatcagtc
     4081 ttcgcaagat tgcaccctgt taatgaggtc gtacttcagg tggttatcgc gcgaatgcgc
     4141 gccttgaaac ggcaagggcc gcaagcataa ggaaaagaag cgcgaatcca gcggccccgg
     4201 ttccattggc gccacccgcc gagcacccgc cgcccttcgt ctcggtgccg ccgtcgtccg
     4261 tttcaacgcc ggtgtcggtg acggtcgaat cggaggtggc ctgatccggg ccaatggtgt
     4321 cccgttcggt tgtgtcgcca accgccccgg agtcgtcaat gacgtcggcg gtcgcgtccg
     4381 gaattggttc aacgatcacg gggcaggcaa ccgtcgcggg gatatcgacg g
//
LOCUS       DAOPPA010000265         4427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21427_length_4427_cov_4.169945, whole genome shotgun sequence.
ACCESSION   DAOPPA010000265 DAOPPA010000000
VERSION     DAOPPA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_4427_cov_4.169945"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKH54_08760"
     CDS             complement(<1..361)
                     /locus_tag="PKH54_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00025.1"
                     /translation="MYSRIQRHKFEAGDDNGFLTARPNCGGDTGVNCNDNVSAQTFAG
                     PYVPADHPQFGKVRTGCAVTLKAYMMNDGPAAQLQNIQPGTCPASFSVNGLPAPFPVT
                     LSPHQLLSIDVTYQPTAV"
     gene            complement(382..1512)
                     /locus_tag="PKH54_08765"
     CDS             complement(382..1512)
                     /locus_tag="PKH54_08765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOD00026.1"
                     /translation="MSAAFILRSVPGRVRAFLSAFLLVSATSAFVAAPVLAREVEPIR
                     LLFVGDLMHHQAQERLAREHRDGLLAGYADRWKFVAPMVSGADIAMANLETAVTGGSA
                     SGFPVFNAPVDFVRAAAATGFDVLSFANNHSMDKGFSGFSATLENIKASGLHAAGDGQ
                     PLYINVRGRTIGVISFTEFVNAGSRFRVSAGKHGPYIVRHDDAPGRAALLQTIRTMRG
                     RADVVVVFVHWGVEYRYRPAIWAKAWAVEMAEAGADLVVGHHPHVVGPSEWVVGTGGR
                     RAFVAYSLGNFSSGMTRDSTPYGYALEVAVGADGSKIPTLRPVWTYRDGANGQWRTYR
                     TVPAAELEADCAEFRTAEVPMRRTGAATHECGRLRGAVNRLP"
     gene            1700..3106
                     /locus_tag="PKH54_08770"
     CDS             1700..3106
                     /locus_tag="PKH54_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD00027.1"
                     /translation="MADAQEQKAKPWYREISQPFIDLIHAPRALWGVNLSYLLEGFVY
                     FGMLGYLAMFFTRHVGLTDPQAGPMVGFLTWGITFSMFLFGGLADKWGVRVAIIGALC
                     LMLVGRGILAGSTYLGLENGLWSPLHLLSMFGLLFIILGYGMYQPAAYAAVKQFMTPK
                     TAGMGYAMLYAVMNLGGWLPSFFSPIRKAVGINGAYGVYTAITVVGLLVTAIILSRKV
                     VARAVAEAKEKLEAELKADGVAAPVESQTPSAAKKPFNVLTWLKNHPLADPKFAFFIF
                     ALIPVQTLFAHNWLTLPMYVSRAFQGSAVGDNFEFAVNLNPLLIFFIVPIVAALTQKK
                     KIYNMMIIGTIVMAAPTFILATGPTVWALAAYLLIMTIGEAMWQPRFLQYAAEIAPPG
                     RTGAYMGVAQFPWFLTKVITSMYSGWFLLRYCPADTPVAGLNTETMWLIYGSIAMVSP
                     VLLIVARGWLGKDFKDKA"
     gene            complement(3122..3730)
                     /locus_tag="PKH54_08775"
     CDS             complement(3122..3730)
                     /locus_tag="PKH54_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00028.1"
                     /translation="MRVGLLVLAVLLVPVLAGFDCSGDGGSGTKLSQCIRLGEAYCDQ
                     EIVAGCVDPSEPCVSQDCYDIFDDKCLATAEEREQVGHDIANIIKPKTECAALRSVED
                     YLLDTVWVMSSSKCANSRAYDSLGKMCGKLLDAYCQKNIDLGCFPVSKSDCVQMALGS
                     GSMPMGPRYNCTTTDDDNPPAPENLAYYNQIMAELKAATVCP"
     gene            3941..>4427
                     /locus_tag="PKH54_08780"
     CDS             3941..>4427
                     /locus_tag="PKH54_08780"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOD00029.1"
                     /translation="MFTENRVLMENSWNTLNFRWSQPALASLLYLVIQVGASAIPIAG
                     WFGSLVIAGPLMLGYVAFMVAFFDRAPDANIEKIFTGFNNFANAFVLQLVMTIFISLW
                     SLLLIIPGIMATYSYSMAFFIMHDDPQVTGTEAIRRSKEMMNGNRWRLFCLDCRFIGW
                     WL"
BASE COUNT          907 a         1339 c         1271 g          910 t
ORIGIN      
        1 caacggccgt cggctgatag gtgacatcga tcgacagcaa ctggtgtggc gaaagcgtga
       61 ccgggaatgg ggccggcagc ccgtttacgc tgaaagacgc cgggcacgtg ccgggctgga
      121 tgttctgaag ctgggcggcc gggccgtcat tcatcatgta tgccttcagc gtgacggcac
      181 agccggtcct gaccttgccg aactggggat gatcggcggg cacatagggg ccggcgaacg
      241 tctgggcaga cacgttgtcg ttgcagttga cgcccgtgtc gccaccacaa ttgggccggg
      301 ccgtcaggaa gccgttatcg tcaccggctt cgaacttatg cctctgaatc cttgagtaca
      361 agcgattttt gactttaatt tttaaggcag gcgattgacc gcacccctaa ggcgaccaca
      421 ctcatgtgtc gcggccccgg tgcgtcgcat cggcacttcg gctgtgcgga attccgcgca
      481 gtccgcttca agttcggcgg ccggcaccgt gcgataggtg cgccactgcc cgtttgcgcc
      541 gtcacggtac gtccagaccg ggcggagcgt cggaattttc gagccgtcgg cgccaaccgc
      601 gacttccagc gcgtatccat aaggcgttga atcccgagtc attccggacg aaaaattccc
      661 aagtgaatac gcgacgaagg cacgccgacc tccggttcca acgacccatt ccgatgggcc
      721 gacaacgtga ggatggtgac ccaccacaag atccgcgccc gcttcggcca tctcgactgc
      781 ccacgccttt gcccatatgg ccggccgata tctgtactcg accccccagt gcacgaaaac
      841 gaccacgacg tccgccctgc cccgcatagt cctgatcgtc tgcagcaggg cggcccttcc
      901 cggtgcgtcg tcatggcgga ctatatacgg gccatgcttg ccggccgaca cgcggaacct
      961 gctgccggca ttgacgaatt cggtaaacga aatgacgccg atcgtccggc cgcgaacatt
     1021 tatgtacaaa ggctgtccat cgccggcggc gtgaagaccg gatgccttta tgttttcaag
     1081 cgtggcggaa aatccactga agcctttgtc catcgaatga ttgttcgcga acgaaagcac
     1141 atcgaagccc gtcgcggcgg ccgcccggac gaaatcgacc ggcgcgttaa agaccggaaa
     1201 accgcttgcg gaaccgcccg ttacggccgt ttcaagattt gccatggcaa tgtcggcgcc
     1261 ggaaaccatc ggtgcgacga atttccagcg gtctgcatac ccggccagca atccgtcgcg
     1321 gtgctcgcgg gcaaggcgct cctgggcctg atggtgcatc aggtcaccaa cgaacagaag
     1381 acggatcggt tcaacttccc tggccagaac cggggcggcg acaaaggcgg acgttgccga
     1441 aaccagaagg aacgccgaca gaaacgcacg cacgcggccg ggaaccgaac gcaaaatgaa
     1501 ggcagccgac atcgacacgc tccatgcgtt actgactgat tgttcggccg gcattttccc
     1561 ctgtcaaatt gatggaaatt tgtggtgcat gaactagact cgaacccgga gaaaaccggg
     1621 cgggtgcaat ccgcccgggg cgggacgctg gacaacgcgg acgggcggat gctggccgcc
     1681 caaaaaaagg acaaaggaca tggcggacgc tcaggaacag aaagcaaagc cctggtacag
     1741 ggaaatctcg caacccttca tcgatctgat tcacgcgcca cgcgcccttt ggggcgtcaa
     1801 tctttcctac ctgctggaag gattcgtcta tttcggcatg ctgggttacc tggcgatgtt
     1861 cttcaccagg catgtcggcc tgaccgaccc gcaggccggt ccgatggtgg gattcctgac
     1921 ctggggcatc acgttctcga tgttcctgtt cggggggctg gccgacaagt ggggcgtgcg
     1981 ggtggccatt atcggggcgt tgtgcctgat gctggtcgga cggggcatcc tggccggcag
     2041 cacctatctt ggacttgaaa acggcctgtg gagcccgctt cacctgctgt cgatgttcgg
     2101 attgctgttc atcatcctcg gttacgggat gtaccagccg gccgcttacg ccgccgtcaa
     2161 gcagttcatg acgccaaaga ccgccggcat gggttacgcg atgctttacg cggtgatgaa
     2221 cctgggcggc tggctgccgt cgttcttttc accaatccgc aaggccgtcg gcatcaacgg
     2281 cgcctatggc gtctatacgg ccatcacggt ggtcgggctg cttgtgaccg cgatcatcct
     2341 ttcgcgcaag gtcgtggcca gggcggtcgc cgaagcgaaa gaaaagcttg aagccgaact
     2401 caaggccgac ggcgtggccg cgcccgtcga atcccagacc ccatccgccg cgaaaaagcc
     2461 gttcaacgtc ctgacctggt tgaagaacca cccgctggcg gatcccaagt tcgcgttctt
     2521 catctttgcc ctgatcccgg ttcagacctt gtttgcccac aactggctga ccctgccgat
     2581 gtatgtctca cgggcattcc agggatcggc cgtcggcgac aactttgaat tcgcggtaaa
     2641 ccttaatccc ctgctgatct tcttcattgt cccgattgtg gccgcgctga cccagaagaa
     2701 gaagatctac aacatgatga tcatcggaac catcgtcatg gcagctccca cattcatcct
     2761 ggcgaccggg cccaccgtgt gggcgcttgc cgcatacctg ctgatcatga ccatcggcga
     2821 agccatgtgg cagccgcgct tcctgcagta cgccgcggaa atcgcgcccc ccggccgtac
     2881 cggtgcatac atgggcgtgg cccagttccc gtggttcctg acgaaagtca tcacgtccat
     2941 gtacagcggc tggttcctgc tcaggtactg ccccgccgac acccctgtcg ccggcctgaa
     3001 caccgaaacg atgtggctga tttacggcag tatcgcgatg gtgtccccgg ttttgcttat
     3061 cgtggcccgc ggctggctgg gaaaggactt caaggacaag gcctgatcat tcgcccatcg
     3121 gttacggaca cacggtcgcg gccttgagtt cagccatgat ctgattgtag tacgccaggt
     3181 tttccggggc gggcgggtta tcatcatcgg tcgtggtgca attgtatcgc ggccccatcg
     3241 gcatcgagcc ggagcctagt gccatctgga cacagtcgga tttcgacacc gggaagcagc
     3301 caaggtcgat attcttctgg cagtacgcgt ccagcagctt gccgcacatc tttcccagcg
     3361 aatcgtaagc cctggaattt gcgcatttgc ttgaactcat gacccagacc gtgtccagaa
     3421 ggtaatcctc aacgctgcgc aaagccgcgc attcggtctt cggtttaatg atattggcga
     3481 tgtcgtgacc gacctgctcg cgttcctcgg cagtcgccag gcacttgtcg tcaaaaatgt
     3541 cgtaacagtc ctgcgacacg cacggctcgg aaggatcgac gcatccggcg acgatttcct
     3601 ggtcgcagta agcttcgcca agtcgaatgc actgagacag cttggttcca gatccgccat
     3661 caccactgca atcgaagccg gccagaaccg gcacaagaag aaccgccaaa accagtaacc
     3721 caactctcat tgacaactcc attttgtatt aacgacatgc gatagtaaca caacaccgtc
     3781 gaaatccgga aaactgggtt cagcattacc cacatccggc acctggttcc gggcacccga
     3841 cgacgcttga ccaccgcggc atggatgcta tgctttgtgg cggtttgcaa tcattgcagt
     3901 catttacgac gatcttcaca aaccaggcat ggaggttccg atgtttacgg aaaacagggt
     3961 gctgatggaa aactcgtgga acacccttaa cttcagatgg agtcaacccg cactggcctc
     4021 gcttctttac ctggtcatcc aggtcggtgc gtccgcgatc ccgatcgccg ggtggtttgg
     4081 aagccttgtc atcgccggac cgctgatgct tggctacgtg gccttcatgg tggctttctt
     4141 tgaccgcgcg ccggatgcaa atatcgaaaa aatcttcacc ggattcaaca acttcgccaa
     4201 cgcgttcgtg cttcagcttg tgatgacgat cttcatatcc ctgtggtcac tgttgctgat
     4261 aatccccggc atcatggcga catattcgta ttcgatggcg ttcttcatca tgcatgacga
     4321 cccgcaggta accggaaccg aagcgatcag gcgcagcaag gagatgatga acggcaacag
     4381 gtggaggctt ttctgccttg actgccggtt catcggctgg tggctgt
//
LOCUS       DAOPPA010000266         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21477_length_4420_cov_4.424971, whole genome shotgun sequence.
ACCESSION   DAOPPA010000266 DAOPPA010000000
VERSION     DAOPPA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21477_length_4420_cov_4.424971"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PKH54_08785"
     CDS             complement(<1..847)
                     /locus_tag="PKH54_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00030.1"
                     /translation="MTSSGSVLWWRAAVAVAFLAAGCGEALPAGNDIVIIRPDTGASA
                     DPGTDDSAEPDAAASDATVRDDAGVIDDAPEIFVTDLSGTDVSRWECDEDEDCPQSGK
                     TCEWVACHPVDHKCYQANSYKSGDMCDEGWPCRKDGHCQGGACVYSYVDCADCGDHVC
                     FDSGENCRDCPQDCGDCPQTDTDCGDLTDNDVDGMTDCEDTDDCGQDPRCSKKTCKDF
                     PEASLTCGQEVELWMSSSPFLDEEELCGFKVGYFVGLYEFVPELGVQARVRITLQDTK
                     ENVRYF"
     gene            complement(844..1476)
                     /locus_tag="PKH54_08790"
     CDS             complement(844..1476)
                     /locus_tag="PKH54_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00031.1"
                     /translation="MVWIMLREFAVVMVVLLMTSSAVRYIWQIRRRQIEPATSTWIIF
                     LAGTALSFITYMLAEDRDFETGILNTMDVLEVSSILVAVLIWANPGGVRFSRFEKWYL
                     AGIGLIIVYGLVFGDAWGSNLFVQGLITAGYIPSVFKVITSRRNTESFLAWTFSLFAG
                     LFALIPAFFDGNTLAIVYSVRTVVLVGSFLSLMAYFQFVYPSRRKNLEKS"
     gene            1642..>4420
                     /locus_tag="PKH54_08795"
     CDS             1642..>4420
                     /locus_tag="PKH54_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00032.1"
                     /translation="MKGFSKVLVAFALVICGCGGPGSPSVGSSLTGAGESAGGMSQSS
                     DMEPVPVEAACVCDFPSDCCDGCNAIEGPCDDGNICSIGDRCIDGMCLGTSQNTCDDN
                     NVCTTDSCLNGYGCIHSNKPGPCSDNSVCTIGDTCVSGVCVPGSTILDCNDGNFCTDD
                     VCDPVAGCQNKATTTYCEDNNPCTRDLCDPMTGCSYEFITAPCNDGDLCTGNDTCVGG
                     VCVGGAGNDCDDGNPCTNDGCDLVTRQCVYTNNTLGCSDGNACTTGDLCAAGKCTSGA
                     PTICDDGNHCTIDSCNVSTGCVFTPKVCNDMDPCTVDSCNLENGNCVFSRKTCDDMNL
                     CTADSCEPVTGACLNMTISCDDGDVCTTDLCHGATGCYHEYNTAVCDDGNACTGSDAC
                     SAGSCVGGPSIDCSDGDRCTIDSCDPVSGCQHSATTGACDDGDACTVDENCADGTCSG
                     GVAVVCDDGNFCTADSCDSATGCVFVATTDSCDDGDACTVGDVCAGGLCAGVLPLDCD
                     DGNVCTDDGCDPVSGCFHVNNTADCEDGTLCTGPDTCVDGACVSGPPVDCNDLNVCTN
                     DSCSPTLGCVHVNTTGPCEDGNLCTTPDKCSKGSCTAGPAADCDDGNECTEDSCDPAV
                     GCVNVPATGPCDDESICTTDDTCVDGVCTGRPALDCDDHDVCTTDRCDPSMGCMYEFN
                     NAACEDGDMCTDGDACSMGACVSGATLVCDDDNVCTDDSCDSSTGCTFAFNSMECDDG
                     DVCTLQDTCLMGVCTGVAFLDCDDENVCTDDACDPVAGCQYSNNTGDCQGGNLCADAK
                     CIDGACYVTAKDCTDDDACTTDGCSLDVGCTHEIIAGCCHESRPCPADETCESGWCSP
                     VGCRACETDGDCGLADARCVTVGQGTFCVTSCATDACDEGWTCQAVGEYPTMCWPDAG
                     WCKCTEPESPSV"
BASE COUNT          879 a         1302 c         1365 g          874 t
ORIGIN      
        1 cgaagtacct gacgttttcc ttcgtatcct gaagggtgat cctgaccctg gcctgcacgc
       61 caagttcggg gacgaattca taaagtccga cgaagtagcc gaccttgaac ccgcagagtt
      121 cctcttcatc caggaatggg ctgctggaca tccacagttc gacttcctgc ccgcaggtca
      181 gggatgcttc cggaaagtcc ttgcacgtct ttttcgagca gcgcggatcc tgcccgcagt
      241 catcggtgtc ttcgcagtcg gtcatgccgt caacgtcgtt atcggtaaga tccccgcagt
      301 cggtgtcggt ctgcgggcaa tcgccacaat cctgcgggca gtcgcggcag ttttcccccg
      361 agtcgaagca gacgtgatcc ccgcagtccg cgcaatcgac gtatgaatag acgcaggcgc
      421 cgccctggca gtggccatcc ttgcggcacg gccagccttc atcgcacatg tcgccggatt
      481 tgtaactgtt ggcctggtag catttgtgat cgaccgggtg gcaggcgacc cattcacagg
      541 tctttcccga ctggggacag tcctcatctt catcgcattc ccatctggaa acgtccgtcc
      601 cggaaaggtc ggtcacgaaa atttccggcg cgtcatcaat gacgccggcg tcatcccgaa
      661 ccgtcgcatc cgatgcggcc gcatccggtt cggccgaatc atctgttccc ggatcggctg
      721 atgccccggt gtcgggacgg atgatgacga tatcgttgcc ggccggcagg gcttccccgc
      781 acccagccgc caggaacgcg acggccaccg ccgcccgcca ccacaggacg cttcccgatg
      841 atgtcatgat ttttccaggt tctttcttct ggaaggatac acgaactgga agtaagccat
      901 cagggaaaga aaactgccga caaggacaac cgtcctgacc gaatacacga tggccagggt
      961 gttgccgtcg aagaaggccg gaatcagggc gaaaagaccg gcgaacaggc tgaaagtcca
     1021 ggcaaggaac gattcggtgt tgcgtcggct ggtgattacc ttgaagacgg atgggatgta
     1081 gccggcggtg atcagtccct ggacgaacag gtttgaaccc caggcatcac cgaaaaccag
     1141 gccgtaaacg attatcaggc cgattccggc caggtaccac ttttcaaaac ggctgaatct
     1201 gacgccgccg ggattggccc atatcaggac ggctaccagg atgctgctga cctccaggac
     1261 atccatcgtg ttcaggatgc ctgtttcgaa gtcacggtct tcggcaagca tgtaagtgat
     1321 gaacgaaagc gccgtcccgg ccagaaaaat tatccaagtg ctggtcgccg gttcaatctg
     1381 ccggcgccgt atctgccaga tgtacctgac ggccgaagag gtcatcagca ggacgaccat
     1441 cacgaccgcg aattcacgaa gcatgatcca aaccatcgac gaactgcgga aggttatccc
     1501 ggaaagcggg ccgcggcaat cgtctggcac atccggcaaa gcccgcccca agtcgcccac
     1561 gaatctgcct gcgtcttcat tacatgttga ctgtcaacgg taattgcata ttctgcccgg
     1621 gtgtatccca tgggggttcc gatgaaggga ttttcgaagg ttctggttgc gttcgcgctt
     1681 gtcatttgcg gttgcggggg gcctggttcg ccgtcggtcg gttcgtcctt gacgggggcc
     1741 ggcgagtccg cgggcgggat gtcccagtca agcgacatgg agccggttcc agttgaagcc
     1801 gcctgtgtgt gtgatttccc gtccgactgc tgcgacggct gcaacgcgat cgagggcccg
     1861 tgcgacgacg gcaacatatg ttcgatcggc gacaggtgca tcgacgggat gtgccttgga
     1921 acaagtcaga acacgtgtga cgacaacaat gtgtgcacca cggattcctg tttgaacgga
     1981 tatggttgca ttcattccaa caagccggga ccttgcagcg acaacagcgt ctgcaccatt
     2041 ggcgacacgt gcgtcagcgg cgtgtgcgtg ccaggttcga ccattcttga ttgcaacgac
     2101 ggaaatttct gcaccgacga cgtctgcgat cctgtcgccg gttgccagaa caaggccacc
     2161 accacgtatt gcgaagacaa caatccctgc accagggatc tgtgcgatcc gatgaccgga
     2221 tgttcatacg aattcataac ggctccctgc aacgacgggg atctgtgcac cggcaacgat
     2281 acctgcgtcg gcggcgtttg tgtcgggggc gcaggcaacg attgcgacga tggcaatccc
     2341 tgcacgaacg atggatgcga tttggtgacc aggcagtgcg tttatacgaa taacaccctt
     2401 gggtgcagcg atggtaacgc atgcactacc ggcgacttgt gcgcggcagg caagtgcacg
     2461 agcggggcgc cgaccatctg cgacgacggc aatcactgca cgattgactc ctgcaatgta
     2521 tcgaccggtt gcgttttcac cccgaaggtt tgcaacgaca tggatccgtg cacggtcgat
     2581 tcctgcaacc tggagaatgg gaattgcgtc ttttcacgga agacctgcga cgacatgaac
     2641 ctttgcacgg ccgattcgtg cgagccggtt accggcgcct gtctgaacat gaccatcagt
     2701 tgcgatgacg gcgatgtctg caccaccgac ctttgccatg gggcgaccgg ttgttaccat
     2761 gagtacaaca cggccgtctg cgacgacggc aatgcatgca ccggaagcga cgcatgctcg
     2821 gcaggatcct gcgtcggcgg tccgtccatt gattgcagcg atggtgatcg atgcaccatc
     2881 gacagttgcg accctgtcag cggttgccag cattcggcga ccaccggcgc gtgtgacgac
     2941 ggagatgcct gtacggtcga tgagaactgc gcggacggca catgttccgg gggagtcgcg
     3001 gtcgtgtgcg acgacggcaa cttctgtacg gccgattcat gcgattcggc cacaggctgc
     3061 gtatttgtcg ccaccacgga ttcatgcgac gacggggatg cgtgcaccgt cggggatgtt
     3121 tgcgccggcg ggttatgtgc cggcgtgctt ccgctggact gcgacgacgg caacgtctgt
     3181 accgatgacg gttgcgatcc cgtgtccggg tgtttccatg tcaacaatac ggccgactgc
     3241 gaggacggca cgctctgcac cggtcccgat acgtgcgttg acggggcctg cgtcagcggg
     3301 ccgccggttg actgcaatga ccttaacgtg tgcacgaacg attcctgttc accgacgctg
     3361 ggttgcgtgc atgtgaacac gaccggccct tgtgaggacg gtaacctgtg cacgaccccg
     3421 gacaagtgct cgaagggttc atgtacggcc gggccggcag ccgactgcga cgacggaaac
     3481 gaatgtaccg aagattcctg cgatcccgct gtcgggtgtg tcaacgttcc ggcaaccggg
     3541 ccctgcgacg acgaaagcat ctgcaccacg gacgatactt gcgttgatgg cgtctgtacc
     3601 ggccggcctg cgctcgattg cgacgaccac gacgtgtgca cgaccgacag atgcgatcct
     3661 tccatgggat gcatgtacga attcaacaat gccgcgtgcg aggacggcga catgtgcacg
     3721 gatggtgacg catgctcaat gggcgcatgc gtatccggtg ccacgctcgt ttgcgacgat
     3781 gacaacgtct gcaccgacga ttcgtgcgac agttcgaccg gctgcacttt tgccttcaac
     3841 tcaatggaat gtgacgacgg cgacgtctgt accctgcagg acacctgcct gatgggcgtc
     3901 tgcaccggcg tggccttcct tgactgtgac gacgaaaatg tctgcaccga cgatgcgtgc
     3961 gacccggtcg ccgggtgcca gtattcgaac aacaccggag actgccaggg tggcaatctt
     4021 tgcgccgacg cgaagtgcat cgatggtgcg tgttacgtga ccgccaagga ctgcaccgac
     4081 gacgacgcgt gcacgaccga cggctgcagc ctggatgtcg ggtgcacgca cgaaatcatt
     4141 gccggctgct gccatgaatc ccgcccctgc ccggccgacg agacgtgcga gtccgggtgg
     4201 tgctcgccgg tcgggtgccg tgcctgcgaa accgacgggg actgcgggct ggccgatgcc
     4261 aggtgcgtga cggtggggca gggaaccttc tgcgtgacgt cttgcgcaac cgatgcgtgc
     4321 gacgagggct ggacgtgtca ggcggtcggc gaatacccga cgatgtgctg gccggacgcc
     4381 ggctggtgca agtgcaccga gcccgagtcg ccgtccgtat
//
LOCUS       DAOPPA010000267         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21651_length_4394_cov_3.758700, whole genome shotgun sequence.
ACCESSION   DAOPPA010000267 DAOPPA010000000
VERSION     DAOPPA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21651_length_4394_cov_3.758700"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKH54_08800"
     CDS             <1..198
                     /locus_tag="PKH54_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00033.1"
                     /translation="GDAANGKQIVKATVPMSECQTYASDLRSMTSDRGSFSMVFDHYE
                     ELPPNLTEKLIAEAKLEEDEE"
     gene            complement(271..1200)
                     /locus_tag="PKH54_08805"
     CDS             complement(271..1200)
                     /locus_tag="PKH54_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00034.1"
                     /translation="MHDQRIFFECDECGRPLECPQPWAGRQVCCPSCGHSGTIPPEST
                     VAGRRVPQVTPSEAMRRPAGVDSTAGGARPGDIVLSSADGFVTEHSLPARSARRPEKQ
                     TLTFRQFWARYKVFIVGGIIVVGTLAWWGSWKWYYRNYVRAEAIMKNRGYGRKLDQPL
                     VPNPEITRLIGQVDVAESRQFADPEDVGLTMELAAAYDAALKEIGHIRGPRAAAILNN
                     AAWLLSTTPHLGLRDPGRALKLAESAVVQTCRKNAGYLDTLAHALAASGRPAEALEVA
                     REALALKPDDQYLKEREREFREAAEGTGNRTGE"
     gene            complement(1205..2830)
                     /locus_tag="PKH54_08810"
     CDS             complement(1205..2830)
                     /locus_tag="PKH54_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00035.1"
                     /translation="MRHDFRFATLLLCAALPCVIACDDGLGRFRPPNGCTPKCDGRSC
                     GTDGCHGSCGECDPGLFCEPVSGQCTGDGKYHVTGNLFIEYMFGDVSLGRVVVSGPDE
                     VPVRDVLVTVIDDQGNILGAGSVEGSDGRFSVPVQEPLSGSEKLVVSTVWAVDGQVVM
                     AVLDSLSEGRDPGKATFDPFVWYVDVPVGGNVGSVTIDIEDGSGALFVFMMNKLAFQQ
                     IVNPLVGGIAGQAIPLAVIFGPADDWYVRCTCYHGLEESHIGTSQGPRLKTMISIVDS
                     PGDASSWGWSVLFHEFGHYILDSYSKDNSPGGYHYIGQELIPKFAFSEGWATFTSLVT
                     ATLWFDDVWPVYWDIQGGSWFFVDFNQGVYHTATGGESYMVFPDVKESTNQELDENWV
                     ARTLYMIWDGSGTTPDPDASMSLEEMAETISSDIFLEGIDWPRDADLFNFLDSALCLY
                     PEKDPALADRVLKEFGYPYQGNPRCSKQDAAYSGIRDLSPGSGPSRRDWTRAPLQTGD
                     RVGVPRAGRTGLPPFVLDGVTIESGIKVGPGRH"
     gene            2995..>4394
                     /locus_tag="PKH54_08815"
     CDS             2995..>4394
                     /locus_tag="PKH54_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00036.1"
                     /translation="MDSPRPAISIRTSRFASAWGLDALSPARFPWPVDWDFSELSGDQ
                     VSESSIRLQFKLRSGMHAVRFTRDFSLDIDIADGSSASVELVTGRARPPVSGGAAGQD
                     PEGSPGAAGDGTDQAGCSVVDLVSVSFDSPLVIRNVFGAVRGAHTLFDNIEVKEFLNR
                     LLAVTGRFAVDLESAASAASERFVLVRKLFDQAAGRVPEVADMARKGFDRLGSAFAAR
                     LGEPVSLEISAVSFRLAPAPDWAGSDCPILVPSITGSFVLSHTGARARFEDIEVPSIL
                     LPRFNSNLTDLLKGNVMVGGAFKKQAVGVSNLMLDVAGMLGAFQGTIDATVALPALRT
                     SWRQADRSDVVAELWTGSSQAKIHAAFQVAKEGDDAVFDVSQLSIGEPDSRRSFKLQA
                     GLTVFGLFRDGLSMPTAVRGTLRQTNRGSLPVTYLRLEGRNPLLKSGNAMIMRIDELK
                     TGGRVDFGFSASGNPV"
BASE COUNT          840 a         1344 c         1328 g          882 t
ORIGIN      
        1 ggcgacgcgg ccaacggcaa gcagattgtc aaggccacgg ttccgatgtc cgaatgtcag
       61 acctacgcgt ccgaccttcg ctcgatgacg tcggatcgcg gctcgttctc gatggtgttt
      121 gatcactacg aggaactgcc gccgaacctg accgaaaagc tgatcgccga agcgaagctg
      181 gaagaagacg aagaatgatt ctggtggggc gggcgaattt tcattcgccc ctacggatcg
      241 tccgggtaac cggtgggggc gggcgaattt tcattcgccc gtacggttgc cagtgccctc
      301 tgccgcctcc cggaattccc tttccctttc cttcaggtat tgatcatccg gcttaagggc
      361 aagcgcctcg cgcgcgactt ccagggcctc ggcgggtctg ccgctggcgg caagcgcatg
      421 ggcaagcgtg tcaagatatc cggcgttctt gcggcaggtc tgcacgaccg cgctttccgc
      481 cagcttcagt gcgcgtcccg ggtcgcgaag ccccaggtgt ggcgtggttg acagcagcca
      541 tgccgcgttg ttcaggatgg ccgcggcccg cgggccgcgg atgtgtccga tttccttcag
      601 ggcagcgtca taggccgccg caagttccat cgtcagtccg acatcctccg ggtcggcgaa
      661 ctgcctggac tcggccacat cgacctgacc aatcaagcgg gtgatttccg ggttcggtac
      721 aagcggttga tccagcttgc ggccatagcc gcgattcttc atgatggctt cagcccggac
      781 atagtttcgg taataccact tccagcttcc ccaccatgca agggtgccga ccacaataat
      841 gcccccaacg atgaagacct tgtagcgtgc ccagaactga cggaatgtaa gcgtctgctt
      901 ttcaggccgg cgggccgatc tggccggcag ggaatgttcc gtcacgaacc cgtcggccga
      961 gctcaggacg atgtcgcccg gccttgcgcc gccggcggtg gaatcaacgc ccgcgggcct
     1021 tctcatcgcc tcggatggag taacctgcgg aacccggcgc cccgcgacag tgctttccgg
     1081 cgggatcgtg ccggaatggc cgcaggacgg gcagcagacc tggcgtcccg cccagggttg
     1141 gggacattcc agcggccgcc cgcattcatc gcattcaaaa aagatcctct ggtcgtgcat
     1201 gctgttaatg ccgccccggc ccgaccttga tgccggactc gatggtcacc ccgtcaagca
     1261 cgaacggcgg aagcccagtt ctgccggccc gcggaacacc cacccgatca ccggtttgca
     1321 gcggcgcccg agtccagtcg cgtcgggaag gccccgatcc aggcgacaga tcccgtattc
     1381 cgctgtatgc ggcgtcctgc ttgctgcacc ggggattgcc ctgataagga tacccgaact
     1441 ccttcagcac ccggtcggca agagcgggat ccttttccgg gtaaaggcaa agcgccgaat
     1501 ccaggaagtt gaaaaggtcg gcatcccgcg gccagtcaat accttcaaga aagatgtccg
     1561 acgaaatggt ttcggccatt tcttcaagcg acatcgacgc gtcgggatcc ggcgtcgttc
     1621 cggagccgtc ccagatcatg tacagcgtcc tggccaccca gttttcatca agctcctggt
     1681 tggtggattc tttcacatcg gggaagacca tgtacgactc gccgccggtc gcggtgtggt
     1741 aaacgccctg gttgaaatcg acgaaaaacc acgacccgcc ctggatgtcc caatagaccg
     1801 gccacacatc gtcaaaccag agcgtcgcgg tgaccagcga cgtgaaggtg gcccatccct
     1861 cgctgaaggc gaacttggga atcagttcct gaccgatgta gtgatacccg cccggactgt
     1921 tgtccttgga atacgagtcc agtatgtaat ggccgaattc gtgaaagaga accgaccacc
     1981 cccaggaaga agcgtcgccc ggcgaatcta caatggatat catggtcttc aatcgcgggc
     2041 cctgggacgt tccgatatgg gactcctcga ggccgtgata gcaggtgcac cggacgtacc
     2101 agtcgtcggc cgggccgaaa atgaccgcaa gcggaatcgc ctgacccgcg atgcccccga
     2161 caagggggtt cacgatctgc tggaatgcca gcttgttcat catgaagacg aagagggcgc
     2221 cggatccatc ctcgatgtcg atggtaacgg aaccgacgtt tccgccgaca ggcacatcga
     2281 cgtaccagac gaaaggatcg aatgtcgcct tgccggggtc gcgcccttcg cttaaggaat
     2341 caagtaccgc catgacgacc tggccgtcaa cggcccagac cgttgaaaca accagcttct
     2401 cgcttcccga caggggttcc tgaacgggaa ccgagaacct gccgtccgag ccttcgaccg
     2461 atccggcccc aagaatgttt ccctgatcgt cgatgactgt gacaagcacg tcgcgtacgg
     2521 gaacttcgtc gggcccggaa acgacgacgc ggcccaggct gacatcgccg aacatgtact
     2581 caatgaaaag gttgccggtc acgtggtatt tcccgtcgcc ggtgcactgg cctgaaaccg
     2641 gctcgcagaa aagcccggga tcgcactcgc cgcaggagcc atgacatccg tccgtcccgc
     2701 aggaacggcc gtcgcacttc ggggtacacc cgttgggcgg cctgaagcgg ccgagtccgt
     2761 catcgcaggc aatcacacac ggaagtgccg cgcacagcag caatgtcgca aagcggaaat
     2821 cgtgacgcat tgcatcaatc cgtaacttca agtaattttg aacgaaccga ccgcgatgat
     2881 atcccgggtt cagtcgttca gtcatcaatt atcccggttt tgtagtaaaa tcccgacctg
     2941 ttttcgtgct tgcccgccgg atctggcggg atgacgcgct ttgcgaggta tcccatggac
     3001 agtccacgcc cggcgatttc gatcaggact tcaaggtttg ccagcgcatg ggggcttgac
     3061 gcgctctcgc cagcaaggtt tccctggccg gttgactggg acttcagcga gctttccggc
     3121 gaccaggtgt ccgaaagcag catcaggtta cagttcaagc ttcggtccgg aatgcatgcg
     3181 gtccgcttca cgcgtgattt ttcgcttgat atcgacattg ccgacggctc gtcggcatcc
     3241 gttgaactgg ttaccggacg ggcgcggccg cctgtttccg gtggggccgc gggccaggat
     3301 cccgaaggat cccccggggc ggcgggcgac ggcaccgacc aggcaggctg ttcggtcgtt
     3361 gacctggtca gcgtcagctt tgacagtccc ctggttatcc gcaacgtttt cggtgccgtc
     3421 cgcggggcgc acactctgtt cgataacatc gaggtgaagg agttcctgaa caggctgctt
     3481 gcggtcaccg ggcggtttgc agtcgatctg gagtcagcag ccagcgccgc atcggagagg
     3541 ttcgtcctcg ttcgcaagct ttttgaccag gcggccggcc gcgtcccgga agtcgccgac
     3601 atggcaagaa agggttttga ccgccttgga agcgccttcg cggcacgact tggcgagccg
     3661 gtctcgctgg agatatcggc ggtcagtttc aggctggccc cggctcctga ctgggctgga
     3721 tcggattgtc ccattcttgt tccttccatt accggttcgt tcgtgctgtc gcataccggc
     3781 gcccgggcca gatttgagga catcgaggtt ccatcaatac ttctgccgcg gttcaattcg
     3841 aatctgaccg accttctgaa gggcaacgtt atggttggcg gggccttcaa gaagcaggct
     3901 gtcggagttt ccaacctcat gctcgatgtg gctggaatgc ttggagcctt tcagggaacc
     3961 atcgatgcga cggtcgccct gccggccctg cgcacgtcct ggcgccaggc cgatcgctct
     4021 gatgttgtcg ccgaactgtg gaccggatcg tcacaggcga agattcacgc cgcattccag
     4081 gttgcgaaag agggtgatga tgccgtattc gacgtgtccc aactctcgat cggcgagccg
     4141 gattcccggc gcagtttcaa gcttcaggcc gggctgaccg ttttcggcct gtttcgcgac
     4201 ggattgtcca tgccgacggc ggttcgggga acattgcgcc agacaaacag gggaagtctt
     4261 ccggtaacgt atcttcgtct ggaaggccgg aatccgcttc tgaagtccgg caacgcgatg
     4321 atcatgcgca tcgacgaact gaagaccggt ggtcgggtgg attttggatt ttccgccagt
     4381 ggcaaccctg tttt
//
LOCUS       DAOPPA010000268         4380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21740_length_4380_cov_3.904971, whole genome shotgun sequence.
ACCESSION   DAOPPA010000268 DAOPPA010000000
VERSION     DAOPPA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21740_length_4380_cov_3.904971"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKH54_08820"
     CDS             complement(<1..543)
                     /locus_tag="PKH54_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00037.1"
                     /translation="MKTTSYLAAALLATVLACGSNPGQTDTNEPQDVTNSDSLADSTA
                     DAVDGIDPDEGRDLAVDQESETVEDTAGELTVPDTVDEDGHDTNDIVEIIEPVPVPPH
                     ALCDMPAYELLPPGSLGDLVEFEPIPLLTLDADTINGLLDGFGFDAMSPVPYGAITYR
                     YRYTTQDRGQQVEATGLMAFP"
     gene            694..1647
                     /locus_tag="PKH54_08825"
     CDS             694..1647
                     /locus_tag="PKH54_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00038.1"
                     /translation="MKRIALLIAVLAGLSVSACEDGGPSDECVPEPMCTPQCPEGYCG
                     DDGCGCPCPACPAGLKCRDQQCVCGICGGDCGECPEIEWISVEQGTFSMGCKADAANV
                     DKICGTPDVLPPHDVSVPAFEIARFETTAALYAQCVATGACLASNTLGSSATGNNPNL
                     WDHPINHVKWFDAEAFCMWIGGRLGSEAEWEYAARGTDGRYYPWGFAGPTCRTAWTGV
                     DGCSINMTHPVGTMPGGKSPWGVDDMAGNVAEWVADHCHTEGYQGAPADGSAWTKEGG
                     GLCRVFRGGGFGAGLEEARSFNREFMSGDLTFKDVGIRCFR"
     gene            1805..>4380
                     /locus_tag="PKH54_08830"
     CDS             1805..>4380
                     /locus_tag="PKH54_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOD00039.1"
                     /translation="MTRQTILAILVVSAFTAGCGGGVQGVDAIDDSAIPDVTIHPDSG
                     TDSGLEDVERDSWLDVGSGDADVQVADVPDDEGFEEVSADGLDAPDYDDSGEDPADVI
                     IDGDAGDDFAGGDDGIEDLTDFGSTDTVDDVIVGRLSVDAPPGGIVLGPALPGCVASP
                     VTVTATNVGNGPLSISLVRLSGGVPFVLSGLPAQWPVVLSGGQTFQFQVGFQPSAAGP
                     HSDAIVIESDSAGGAEHTIGLFGQSVVCGSGTHKCGCACMSDSSVDSCGSRCTACPTG
                     GVGTTPVCESSGSSWVCGFKCDDGYHRCGSSCKLDSDPNACGASCTLCVSKANSTRVC
                     VDGLCALDCNDGWGDCDERYDTGCEESLATVLNCGICDRACTSAPANGSPACIQGDCS
                     FSCKSGYHKEGSACVLNNVDSCCGDDGPGGSCIDCTAMLAPMPPNTTGRCVGLIDFTC
                     ELICQPGWFSTSGGINDGCECHFVSDFDQPGNGVDENCDGSDGRPDLAWYVSTTGLDS
                     NAGTAAAPFRTIQRAVNAARADFIKKRVFIAAGTYNEGVVIDGSISLYGGLSADGNWT
                     WAESNVTTIRATVPVGTRIIAVDGVNMNEPTWIGNMTLWAGPEVQGGDGSDVYGVKCS
                     KCPGLRLHGLTIIAGPGGNVDGVPIGATGTAGGTGGTGGYFGDPGAAGTSGCSRPGGL
                     GGLGGVFSSGYDGAFGVVVDGGGGYGGSGGWYSDGEVGGVGAGCSDVASGGGGAQTIG
                     GASAGYWLGTAGTTGGQGCHGHGGGGGGGAMAYYDGDYYYGGGGGGGGGGGGGPPAMP
                     AAPTWGGAMNQPWLPWTDWSQTPWAGLDPALSAEGMQWFNTMLPWLQSWQQNQQWMNE
                     LA"
BASE COUNT          756 a         1213 c         1506 g          905 t
ORIGIN      
        1 ggggaacgcc atcagaccgg ttgcctcgac ctgctggccg cgatcctgtg tcgtgtaacg
       61 ataccggtac gttatcgcac cgtacggaac cgggctcatc gcgtcgaagc caaacccgtc
      121 aagcaacccg ttgattgtgt ccgcatccag tgtcagcagg ggaatcggct cgaattccac
      181 caggtcgccc agcgatccgg ggggaagcag ttcataggcc ggcatgtcgc aaagggcatg
      241 gggcggcacc ggaacaggct cgattatttc aacgatgtcg ttcgtgtcgt gcccgtcctc
      301 gtccacggtg tcgggaaccg tcagttcgcc ggcggtatcc tcaaccgttt cggattcctg
      361 atcgaccgcc aggtcgcgac cttcgtccgg atcgatgccg tcaaccgcat ctgccgtcga
      421 atcagccagg gaatcgctgt tggttacatc ctgtggttcg ttcgtgtcgg tctggccggg
      481 attcgacccg caggcaagaa ccgtcgctag cagggcggcg gcaaggtaac tggttgtttt
      541 catttgggct tcccttgtaa gtccactatc attgcccacg ccggcgcgcc gactggcggg
      601 cgtactttac aacatcgagg ccggataatt caattgacgg gcccggtgca gttttttaca
      661 gtttcgactg cctctgtctg cgacgaagcc ttgatgaaaa gaattgcctt gctgattgcg
      721 gttctcgccg gtctttctgt ttctgcctgt gaagatggcg ggccttccga cgaatgcgtg
      781 cccgagccga tgtgcactcc gcaatgtccg gaaggttact gtggcgacga cggctgcggc
      841 tgcccctgtc ccgcctgccc ggccggcctt aagtgcaggg atcagcagtg cgtatgcggg
      901 atttgcgggg gtgactgcgg cgagtgtccc gaaatcgagt ggatttcggt cgagcagggt
      961 accttttcaa tgggctgcaa ggccgatgcg gcgaatgtcg ataagatctg cggcaccccg
     1021 gacgttctcc cgccccacga cgtatccgtg ccggcatttg aaattgccag attcgaaacg
     1081 accgcggctc tttatgcgca gtgcgttgca acgggggcct gcctggcttc gaacacgctt
     1141 ggcagcagcg ccaccggcaa taatccaaat ctgtgggatc atcccatcaa tcatgtgaaa
     1201 tggttcgatg ccgaggcctt ctgcatgtgg atcggcgggc gccttggttc cgaggccgaa
     1261 tgggaatacg cggcaagggg aaccgatggt cggtattacc catggggttt tgcggggccg
     1321 acctgcagga cggcgtggac cggcgtcgat ggatgctcga ttaacatgac acatcccgtg
     1381 ggcaccatgc cgggcggcaa gtccccctgg ggggtcgatg acatggccgg aaacgtggcc
     1441 gagtgggtgg ccgaccattg tcacaccgaa ggataccagg gtgccccggc cgacggttcc
     1501 gcgtggacga aggagggggg cggactttgc agggtgttcc gtggcggtgg cttcggtgcc
     1561 ggtctcgagg aagccaggtc cttcaatcgt gagttcatgt cgggcgatct gaccttcaag
     1621 gatgttggga ttcgctgctt ccgctaaacc attcgttccc tcgcttgtat tgatcccgga
     1681 ttgtaaattc gcgtttgcac ataaccgata tgcgcaataa aattgacgca gttcagcatt
     1741 caggccacct gtatccaagg gcttttgttg accggtcgag agccggtcgt ccccgggggg
     1801 attcatgaca aggcagacta ttcttgcgat tctggttgtg tccgcgttca cggcaggctg
     1861 cggcgggggt gtccaggggg ttgatgcgat cgacgattcg gcaattcccg atgtcacaat
     1921 ccatcccgat tcagggacgg attcgggcct ggaagacgtt gaacgcgatt cctggctgga
     1981 tgtcggttcc ggggatgccg atgttcaagt tgcggacgtg cccgatgacg aaggttttga
     2041 agaagtgtcc gccgacggtc tcgatgcgcc ggattacgac gattcgggcg aagatccggc
     2101 tgatgtcatc attgacggcg acgctggcga tgactttgcg ggcggggatg acggcatcga
     2161 ggatttaacc gatttcggtt cgacggacac tgtcgatgac gtaatcgtcg ggcggctttc
     2221 ggttgacgcg cctccgggcg gtatcgtgct ggggccggcg cttccggggt gtgtcgcgag
     2281 tccggtgacc gtcaccgcga caaacgtcgg caacgggccg ctgtcgattt ccctggttcg
     2341 tctttccggg ggcgtcccgt ttgtgctgtc cgggctgccg gcccagtggc cggtggtgct
     2401 gtccggtggt cagacgttcc agttccaggt tggtttccag ccatcggctg ccgggcccca
     2461 ctccgatgcc atagttatcg aaagcgattc ggccggtggc gccgaacaca cgattggact
     2521 gttcgggcag tcggtcgtct gcggttccgg aacccacaag tgcggctgcg catgcatgtc
     2581 ggacagttcg gttgacagtt gcggaagccg ttgcacggct tgtccgactg gcggtgtcgg
     2641 aaccacgccg gtctgcgagt ccagcggttc gtcgtgggtg tgcggcttca agtgcgacga
     2701 cggttaccat cgttgcggtt caagctgcaa gctggacagc gatccgaatg cgtgcggcgc
     2761 ttcgtgcacg ttgtgcgttt caaaggctaa ctcgacacgc gtctgcgtcg atggcctgtg
     2821 cgcgcttgat tgcaacgacg ggtggggcga ctgcgacgaa aggtacgaca ccggatgcga
     2881 ggaaagtctt gcaacggtcc tgaactgtgg aatttgcgac cgcgcctgca cgagcgcccc
     2941 tgccaacggt tcgccggcct gtatccaggg cgactgctcg ttttcatgca agtccgggta
     3001 ccacaaggaa ggcagcgcgt gcgtcctgaa caatgtcgat tcctgctgcg gtgacgacgg
     3061 ccccggtgga agctgtatcg actgcaccgc gatgctggcg ccgatgccgc cgaacaccac
     3121 cggcagatgc gtcggactta ttgattttac ctgtgaattg atttgccagc cgggctggtt
     3181 cagcaccagc ggtggcataa acgacggatg cgaatgccat ttcgtgtcgg atttcgatca
     3241 gccgggaaac ggcgtcgatg aaaactgcga cggcagcgac ggaaggcccg atctggcctg
     3301 gtacgtttcc accaccggtt tggattcgaa cgcgggaacc gccgcggcgc cgttcaggac
     3361 aatccagcgg gccgtgaacg cggcccgggc cgacttcatc aagaagcggg tattcatcgc
     3421 ggccgggacg tataacgaag gcgtggttat cgacggctcc atttcgcttt acggcgggct
     3481 ttccgcagac ggcaactgga cgtgggcgga atcgaacgtc acaaccatcc gtgccaccgt
     3541 tcccgtgggc accaggataa tcgcggttga cggcgtcaac atgaatgagc cgacctggat
     3601 cggcaacatg actttgtggg ccggacccga ggttcagggc ggggacggtt cggacgttta
     3661 tggcgtcaag tgctcgaagt gtcccggatt gaggctgcac ggactgacga taatcgcggg
     3721 accagggggc aacgttgacg gcgtgccgat cggggcaact ggaaccgccg gtggcactgg
     3781 cggcactggc ggatactttg gcgatccggg tgccgccggg acgtccgggt gctccaggcc
     3841 tggcggactt ggtggactgg ggggcgtctt cagttcggga tatgacgggg ctttcggcgt
     3901 tgtggtcgat ggcggcggcg gctacggtgg cagcggtggc tggtacagcg acggggaggt
     3961 cggcggcgtt ggtgccggtt gcagcgatgt ggcgtcgggc ggtggcgggg cacagacgat
     4021 cgggggcgct tcggccggtt actggctggg cacagccggc acgacgggtg gccaggggtg
     4081 tcatggacat ggtggcggcg gcggcggtgg cgccatggcc tactatgacg gtgattatta
     4141 ctacggcggt ggcggcggcg gcggtggcgg tggcggtggc ggaccgccag ccatgccagc
     4201 cgcgccgacg tggggtgggg cgatgaacca accctggttg ccgtggacgg actggagcca
     4261 gacgccctgg gcgggcctcg acccggcgct gagcgccgag ggcatgcagt ggttcaacac
     4321 gatgctaccg tggctacaat cgtggcaaca gaatcagcag tggatgaacg agctggcaca
//
LOCUS       DAOPPA010000269         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21744_length_4379_cov_3.877197, whole genome shotgun sequence.
ACCESSION   DAOPPA010000269 DAOPPA010000000
VERSION     DAOPPA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21744_length_4379_cov_3.877197"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKH54_08835"
     CDS             complement(<1..634)
                     /locus_tag="PKH54_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HOD00040.1"
                     /translation="MNSERTEIYLVPGFFGFKSLGALQYFNGVPDVLATQLSARGVVA
                     RIHECEVLPAGSISRRAESVFETVQKTGGLQADHIHFIGHSTGGLDVRLLLSRCGGMN
                     GNWGRILEKTRTVVTISTPHHGTPLANFFLTAQGKQILKLMTSAATTDSGRRTLHILA
                     RLASFAARLDDPLRPADSWLDVATEKFFGRISSDRDDPLFKFISAVAYDQG"
     gene            complement(642..2870)
                     /locus_tag="PKH54_08840"
     CDS             complement(642..2870)
                     /locus_tag="PKH54_08840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOD00041.1"
                     /translation="MRSGFIGILALVATVSCQKTQDSTFEVVDLAAHVRPHVGTSGYG
                     NVFVGASWPRGMVKVGPNTIVKPGSMSGYDYNNERIKGFVHSHLEGPGGAGNGYNRIL
                     VMPFVGSPEPTEESWSSTFSHDEEVVSPDYYSVRLASSGVLAELTASRLVGLHRWTYP
                     AAPRAGVILDLQTSLGQWLESDLEVVGTDTVEGWSRYQTNGLISMFADLVDPGTGIST
                     VYYSIKLDRPVDVSEWNPASHPGAFLVFSTEEGDVVEMRVGISYISVDQARQNREAID
                     GMTFEDVRAETRGQWNRLLSRVRIESDRPDDVDRFYTAFYHSLLAPADYTEGNRFFSG
                     ADGKGQTWLADGWRYYTDDWCMWDTYRTSHPWFSIIEPEVNSDMIRSIVHTYEAGGWM
                     QKCTWNATGDSRCMTANPQFCVIADGFLKGFDDFDIDLAWEGMKKGSLQDSENPLPDG
                     MCGYFNRGTPPDYVDLGFVSMDCDVDQSASMTLEHAHNDWCVARFARAIGQDEWADFF
                     LARSMNYVNHWNQEAGFMVPKNRDGSWLQDFDPVGGAGFCEADSWKYTWSVPQDVCGL
                     VELMGGPENFVAKLDQFFDGGHFAMDNQPDFHVPWLYAFAGAAARTQETVSDLMARHF
                     HLGPDGLPGNDDAGSMSTWLVFASLGFYPVTPGGDTYIMNAPLFDRVELFIDPEGEAR
                     RFEIVAQGLDAESRYIQSATLNGKPLEFPEISHSDIVAGGRLVLKMGPEPSAWGTAIC
                     DR"
     gene            complement(2870..3424)
                     /locus_tag="PKH54_08845"
     CDS             complement(2870..3424)
                     /locus_tag="PKH54_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00042.1"
                     /translation="MAAAEGQAGLSRIRGLGVPFAVAFLSIVITLAVAAFLVARRKAE
                     ARKAVAWLRARYGRDLSLLAGCLLLDGRRRIPAVAAIDATRIVWRGVRFDAGTVGEIE
                     LASVSMVIWADAVRGAHPAIWRRLPGQVLSVIDLDGVERHFVFAEAQAVLWQKVLEIG
                     LSDDDATSLKHVAEPEVVSIGGGV"
     gene            3507..>4379
                     /locus_tag="PKH54_08850"
     CDS             3507..>4379
                     /locus_tag="PKH54_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00043.1"
                     /translation="MPPSSPPEPFPLLLVSLLLAAAVLAPTVTVIAVRSSGITARHKA
                     ALLAATAIFGLFAAFKTPIALNPDSFVIMQDGTSLQNLKHVHGGGVHSSPFTSQLARL
                     VAPEGDAVRLPSLIRLNRMISIAACTIFFGIAAFITGSALAAIPISWIFLSNRNMSLV
                     SSSEFPGPGITVIFLLVFIAGFAISRLRGRRAFEAIPALALIAVSGMIAAGLRPEAGA
                     AVLVVLAIAALVAVMPPARLHAMGNALSRFAADAARRPREALEALIAALVFFGTAFAL
                     ERMLSPPASWVAGAF"
BASE COUNT          896 a         1393 c         1251 g          839 t
ORIGIN      
        1 caccctgatc gtaggcgact gccgatatga acttgaaaag cggatcatcc ctgtcggacg
       61 agatccggcc gaaaaacttc tcggttgcga catccagcca tgaatccgcc gggcgcaggg
      121 gatcgtccag gcgggccgcg aaactggcca gccgtgccag gatgtggagc gtccgacgcc
      181 ccgaatccgt ggttgcggcg ctggtcatca gcttgagaat ctgcttaccc tgggcggtaa
      241 gaaagaagtt ggcaagaggg gttccgtgat gaggcgtcga aatcgttaca acggttctgg
      301 ttttttccag gatacggccc cagtttccat tcattccacc gcacctcgac agcagcagcc
      361 tgacgtcaag accgccggtc gaatgtccga taaaatgaat gtggtctgcc tggaggcccc
      421 ccgtcttctg gacggtctcg aacacgcttt cagccctgcg gctgatcgag ccggccggaa
      481 gaacctcgca ttcgtgaatc cgcgccacca cgccccttgc cgaaagctgc gtcgcaagca
      541 catcgggaac gccattgaag tattgaaggg cgccaagaga cttgaaaccg aagaatcccg
      601 gcaccagata tatctcggtt cgttccgaat tcattgaaca atcacctgtc gcagatggcg
      661 gtgccccagg ccgacggctc gggacccatc ttcaacacca gtctgccgcc cgcaacgatg
      721 tcggaatggg aaatttcagg aaattccagc ggctttccgt tcagggtcgc tgactggatg
      781 taccggcttt cggcatccag tccctgggcc acgatctcga acctgcgcgc ttcaccctcg
      841 ggatcaatga aaagctcgac ccggtcaaac aggggggcgt tcatgatgta ggtgtcgccg
      901 cccggcgtga ccggatagaa acccagcgac gcaaagacca gccaggttga catcgaaccg
      961 gcatcgtcgt tcccgggcag tccgtcggga ccaagatgga aatggcgcgc catcagatcg
     1021 gaaacggtct cctgggtccg ggcggccgcc ccggcaaagg cataaagcca cgggacatga
     1081 aaatccggct ggttgtccat cgcgaaatgg ccgccgtcaa aaaactgatc cagttttgcg
     1141 acaaagtttt cggggccacc catcaactcg acaaggccgc acacgtcctg cggcacggac
     1201 catgtgtact tccaggaatc ggcctcgcag aatcccgcgc cgccaaccgg atcgaaatcc
     1261 tgaagccacg atccgtcgcg attcttcggc accatgaatc ctgcttcctg attccagtgg
     1321 ttcacatagt tcatcgaccg ggcgaggaaa aagtcggccc attcatcctg cccgatcgcc
     1381 ctggcgaacc tcgccacgca ccagtcgttg tgggcgtgtt caagcgtcat ggacgccgac
     1441 tgatcgacgt cgcagtccat cgacacgaag ccaagatcga cataatccgg gggagttccg
     1501 cggttgaagt atccgcacat tccatctgga agcggatttt cggaatcctg cagggatccc
     1561 tttttcatgc cctcccaggc cagatcaata tcgaaatcgt caaacccctt caggaatccg
     1621 tcggcgatca cgcagaactg cggattggcg gtcatgcacc tggaatcgcc ggtcgcgttc
     1681 cacgtacact tctgcatcca gcctcccgcc tcgtacgtat gcacgatcga gcggatcatg
     1741 tcggaattga cttcgggctc gatgattgaa aaccagggat gggaagtgcg gtatgtgtcc
     1801 cacatgcacc agtcgtcggt gtagtaccgc cagccatccg cgagccaggt ctggcccttg
     1861 ccgtccgcgc cggaaaagaa cctgtttccc tcggtgtaat cggccggggc aagaagcgaa
     1921 tggtagaagg cggtatagaa gcgatccacg tcatccggcc gatcggattc aatccttacc
     1981 ctggaaagca ggcggttcca ctgccccctg gtctcggcac ggacatcctc gaacgtcatg
     2041 ccgtcgatgg cttccctgtt ctgcctggcc tgatcgaccg aaatatagga gatgccgacc
     2101 cgcatctcga caacgtcgcc ctcctcggtg gaaaagacca ggaaggcccc ggggtgcgat
     2161 gccggattcc attcggacac gtcaacggga cgatccagct tgattgaata ataaacggtc
     2221 gaaatgccgg ttccgggatc aaccagatcc gcaaacatgc ttatcagacc gttcgtctgg
     2281 tacctgctcc agccttcgac cgtgtccgtg ccgacgactt caaggtcgga ctcaagccac
     2341 tggcccagcg acgtctgaag gtcgaggatt acgccggccc tgggggctgc cgggtatgtc
     2401 cacctgtgca ggccgaccag tcgtgaagcg gtcagttcgg caaggacgcc ggatgaagcc
     2461 agcctgaccg agtagtagtc cgggctgaca acctcctcgt catgcgaaaa tgttgatgac
     2521 cacgattcct cggtcggttc gggcgagccg acgaaaggca tgaccaggat ccggttgtag
     2581 ccgtttccag cgccgccggg gccttccaga tgggagtgaa caaagccctt gatcctttca
     2641 ttgttgtaat cgtaacccga catggaaccg ggcttcacta tggtgttcgg cccgaccttg
     2701 accatccctc gcggccagga agcgccgacg aagacgttgc cgtaaccgga cgtcccgacg
     2761 tgcggacgca catgcgccgc cagatcgacc acttcgaatg tagaatcctg ggtcttctgg
     2821 catgagaccg tcgcgacaag cgcaagaatg ccgatgaatc ccgaacgcat cagacgccac
     2881 ctccaatgga aacgacttcc ggctccgcca catgcttcag ggaggtggca tcgtcgtcgg
     2941 aaagcccgat ttcaagaacc ttctgccaca gaacggcctg ggcctcggca aaaacgaaat
     3001 gtctttcaac gccgtccaga tcaatcacgc tcaaaacctg tccgggaaga cgccgccaga
     3061 tggcggggtg agccccgcgg acggcgtcgg cccagattac catcgacacc gaggccagtt
     3121 cgatctcgcc cactgttccg gcgtcgaacc ttacgccgcg ccagacgatc cttgtcgcgt
     3181 caatcgccgc gaccgccgga atccgcctgc ggccgtcaag aagaagacac ccggcaagaa
     3241 gggacaaatc ccggccgtac ctggcgcgca gccacgcaac agccttcctg gcctcggcct
     3301 tgcgcctggc gaccagaaag gccgccaccg ccaaagtaat cacaatagaa agaaacgcca
     3361 ccgcaaatgg cacgccaagc ccccggattc gcgacagtcc cgcctgccct tcagcggctg
     3421 ccacgataac ccggattcgt agtaatatcc agatgcgacc gtcccccgtg gcggcgcaat
     3481 gataatcccg ggatacagga atcaggatgc cgccgtcgtc accaccggaa ccgtttcccc
     3541 tgctgctggt ttcactgctg ctggccgcgg cggtgctggc tccgactgtg acggtcatcg
     3601 cggtccgttc cagcggcatc acggcccggc acaaggccgc attgcttgca gccaccgcca
     3661 tattcgggct gtttgcggca ttcaagaccc cgatcgccct gaatcccgat tccttcgtga
     3721 tcatgcagga cggaacttcg ctccagaact tgaagcacgt ccatggcggt ggcgttcaca
     3781 gcagcccatt cacatcccag cttgccaggc tggtcgcccc ggaaggcgac gcggtgcggc
     3841 ttccctcgct gattcgattg aacaggatga tttcgattgc ggcgtgcacg atctttttcg
     3901 gcatcgccgc tttcatcacc ggatccgcgc ttgcggcgat tccgatttcc tggatattcc
     3961 tgtcgaacag gaacatgtca ctggtttcgt cgtcggaatt tcccggccca ggcatcaccg
     4021 tcattttcct ccttgttttt atcgcgggct tcgcgatctc aaggctccgg ggcaggcggg
     4081 cgttcgaggc gatcccggcg cttgccctga tcgcggtcag cggaatgatc gcggccggcc
     4141 tgcggccgga agccggcgcg gccgtccttg tcgtgcttgc gatcgcggct ttggtcgcgg
     4201 tcatgccccc ggccaggctt cacgcgatgg ggaatgcctt gtcgcgcttt gccgcggacg
     4261 ccgcccgacg cccacgcgaa gcgcttgagg cgctgattgc cgcgctcgtc ttcttcggca
     4321 cggctttcgc cctggaaagg atgctgagtc cgccggcaag ctgggttgcc ggggcgttc
//
LOCUS       DAOPPA010000270         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_21754_length_4377_cov_3.986349, whole genome shotgun sequence.
ACCESSION   DAOPPA010000270 DAOPPA010000000
VERSION     DAOPPA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21754_length_4377_cov_3.986349"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKH54_08855"
     CDS             <1..1072
                     /locus_tag="PKH54_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00044.1"
                     /translation="MLTGRTPFDADNPLAILMKHIQDSVPPMASVRPDVSVPPEVEEM
                     VRRCLEKSADRRFQSAAALAAEARAVLDRLDGRYERVVFVDKQATGQADQSPSEPAEV
                     PLPARRRRGPAGLLIAVSVLAVLGTASGIGWTMFMRDSAATPSDAAVVIPDPAAPPLS
                     PPAPVAALAPDAGSQKDAVAQAGSPPVVQAQVKVRFESVPAGARVLLDGVELGLTPFE
                     EKMPVEAEPMDFVFRLAGHSDSSVSSRLERDTTLSAVLSPVPEAPRVQQEGRARPAAG
                     VRRQPPQTAASVAPAPSASAPASTPVAATPSPPAAPQAAPAPAPEKPSKPAGDGGFRK
                     VGGDQSPAGKNAPGKVGDLKKF"
     gene            1269..2207
                     /locus_tag="PKH54_08860"
     CDS             1269..2207
                     /locus_tag="PKH54_08860"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOD00045.1"
                     /translation="MVKKLKWIWMDGRLVPWDDANVHILSHTMHYGVGAFEGIRAYHH
                     EDGRSAVFRLAEHTQRLIDSAHLLELKVPYTREQIDQACMQTLAANGMEAGYLRPLVY
                     LGDKEVGVYPGNDPDVRLSVIAWRWGAYLGPEALEKGIRVKISTWTKHHVNALLVKGK
                     ICGHYVNSVLAKVEAKREGFDEGILLDHCGYVAEGSGENLFIVKNGEIFTPDLSSSIL
                     GGITRDTVITLAKAEGIKVNEVRMGRSDLYLADEVFFSGTAAEVTPIYEIDRRQVGAG
                     KAGPITRMLQQKYMKLVAGGVQGYDKWFSFYTPKEI"
     gene            2213..2920
                     /gene="rsmI"
                     /locus_tag="PKH54_08865"
     CDS             2213..2920
                     /gene="rsmI"
                     /locus_tag="PKH54_08865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD00046.1"
                     /translation="MPGTLYIVATPIGNLQDITLRALAILREVPVLACEDTRHTGMLL
                     KALEIDRTGRRLIAFHDMNERRQVPLLLSILQEGQDVALVSDAGTPLVSDPGYRIVSE
                     AAAAGISVVPIPGPCSAITALSASGLPSDRFTFMGFVAPKSPRRMKAFAELGRMTGTA
                     IFFVPARTLEKVLAEIGLAIPGATVVVARELTKLHEEFVRGSPEACAAAFSGRTPRGE
                     VTLLVSPPRDREDRPVE"
     gene            3063..>4377
                     /locus_tag="PKH54_08870"
     CDS             3063..>4377
                     /locus_tag="PKH54_08870"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOD00047.1"
                     /translation="MSNRFFISLLAPFAAFSMTMTGCGQESSPDDLNGAMDVGYELQE
                     PDSIDPDSGDQDVPDNLCRLDALCDVQDDVGPGSPGTFCEENSDCEYGFCLPYIYGNI
                     CSSPCSSSCPFPGWTCEALVLAGGDTTYVCLPPATGLCKPCMTDAACRLVEGEESARC
                     VSYGEEASFCATDCTANPESCLEGFECREVEAGFSACVKEDGLCPCVSPVAGMESRCS
                     RSNQSGTCFGSRFCTRTGNEFAWGACSVGEPSQEVCNRIDDDCNGATDDMGTEVCGTG
                     VCAHMVEKCVDGQAFLCDPYEGSQDEVCNGLDDNCNGTTDENWPDKGAACDGGDTDVC
                     ANGTWGCAEDGMGLTCEGDIASDVELCNGVDDDCDGKTDEPEDLGTIECGLGACHRVL
                     PACTSGVPTECDPMLGAVSPDIPDIDMDDVNCDGVDGDAPSAVFVD"
BASE COUNT          788 a         1282 c         1414 g          893 t
ORIGIN      
        1 gatgctgacc ggccgcacgc ctttcgatgc cgataatccg ctggccatcc tgatgaagca
       61 catccaggac tccgttccgc cgatggcttc cgtgcgtccc gatgtttcgg tgccccccga
      121 agtggaggaa atggttcgcc gctgcctgga gaagtcggcg gacaggcgtt tccagtcggc
      181 ggcggccctg gctgccgagg cgcgcgccgt gcttgatcgg ctcgacggca ggtacgaacg
      241 ggttgttttt gtcgataagc aggcgaccgg tcaggccgac cagtcgcctt ccgaacctgc
      301 ggaagtcccc cttccggcca ggcgccgacg cggcccggcc ggtctgctga ttgccgtttc
      361 cgtgctggca gtgcttggga cggcttcggg catcggctgg accatgttca tgcgggattc
      421 cgccgcgacc ccgtccgacg cggccgttgt gattccggat ccggctgcgc ccccgttgtc
      481 gccgccggcg ccggtcgccg cgcttgcccc ggatgccgga tcgcaaaagg acgcggtcgc
      541 ccaggccggg agtccccccg ttgtgcaggc ccaggtcaaa gtcaggttcg agtcggttcc
      601 cgcaggcgcg agagtccttc ttgatggcgt ggagcttgga ttgactcctt tcgaggaaaa
      661 gatgccggtc gaggccgaac cgatggactt cgtgttcagg cttgcgggcc attcggattc
      721 gtccgtcagt tccaggctgg agcgggacac cactctttcg gcagtccttt caccggttcc
      781 cgaagcgcca cgggtgcagc aggaaggacg cgccagaccg gcggccggtg tccgcaggca
      841 accgccgcag accgccgcgt cggtcgcccc cgccccatcg gcctcggcgc ccgcgtcaac
      901 cccggtggcg gcgactccgt cacccccggc cgccccgcag gcggcgcccg cgcccgcacc
      961 cgaaaagcca tcgaagcctg ccggcgacgg cggtttcagg aaggtcgggg gcgatcagtc
     1021 gccggcgggg aaaaatgctc caggcaaggt cggggacttg aagaagttct agcaattgtg
     1081 cccgcctcac ttccaatctt cctgctttcg tttcgcccga tatcgctgca atcgttgctt
     1141 caaggggtgt tgaaatagat ttgaatccca aataggatgc atccttgatg tccgggaagc
     1201 cgtcggcttg atgcggcatg tccggccatc tgcaattaac tgtattggtg gattggaggc
     1261 gtttcgtcat ggttaagaag ctcaagtgga tctggatgga cggcaggctg gtgccctggg
     1321 atgacgcgaa tgttcacatt ctttcgcaca cgatgcatta cggcgtcggc gcgttcgagg
     1381 gaatccgcgc atatcatcac gaggacggcc gttccgccgt tttccggctt gcggaacata
     1441 cccagcgcct gatcgattcc gcgcacctgc tcgagctgaa ggttccgtac acacgtgaac
     1501 agattgacca ggcgtgcatg cagacgcttg cggcgaacgg aatggaagcc ggatacctcc
     1561 ggccgctcgt ttatctcggc gacaaggaag tcggcgtata tccaggtaat gatcccgatg
     1621 tcaggctcag cgtcatcgcc tggcgatggg gcgcctatct tggaccggag gcgctggaaa
     1681 agggcatccg ggtgaagatt tccacgtgga ccaagcatca tgtcaacgca ctgctggtca
     1741 aggggaagat ttgcggacac tacgtcaatt ccgtgctggc caaggtcgag gcaaagcgcg
     1801 agggctttga cgagggcatc ctgctggatc actgcggata cgtggccgag ggttcaggcg
     1861 agaacctgtt catcgtgaag aatggcgaga tttttacccc ggatctttcc tcgtcgattc
     1921 ttggcggcat tacaagggac acggtgatta cgcttgcgaa ggcggaagga atcaaggtca
     1981 acgaagttcg gatgggccga agcgatcttt acctggccga cgaagtcttc ttttcgggga
     2041 cggcggcaga agtcaccccg atttatgaaa tcgacaggcg tcaggtcggt gccggcaagg
     2101 caggtcccat cacccggatg ctccagcaga agtacatgaa gctggttgcg ggtggcgttc
     2161 agggatacga caagtggttc tcgttctata cccccaaaga gatctgactt gaatgcccgg
     2221 aaccctctac atcgtggcca ccccgatagg taatcttcag gacatcacat tgcgggcgct
     2281 tgcgatcctt cgcgaggttc cggttctcgc ctgcgaggac acgcggcaca cggggatgct
     2341 cctgaaggcc ctggagatcg accggaccgg tcgccgcctg attgcctttc atgacatgaa
     2401 tgaacgcagg caggttccgc tgctgctctc gattcttcag gaaggccagg acgtggcgct
     2461 tgtttccgat gccgggacgc cccttgtcag cgatcccgga tacaggattg tttccgaggc
     2521 tgccgcggcc ggaatctccg tggttcccat tcccgggcca tgttctgcga tcacggcgct
     2581 ttcggcctcc ggactgcctt cggatcgctt cacgttcatg ggttttgttg ctccgaaatc
     2641 acccaggcga atgaaggctt ttgccgaact cggccgaatg accgggacgg ccatcttctt
     2701 cgtgccggcc cgcacgctcg aaaaggttct ggcggagatc gggctggcca ttcccggggc
     2761 aaccgttgtc gttgcgaggg aactcaccaa gttgcacgag gaattcgtca ggggttcgcc
     2821 ggaagcctgt gccgcggctt tctccggccg cacccccagg ggcgaggtca cccttcttgt
     2881 atcacccccg cgggatcgcg aggatcggcc ggtcgaatga tccggccgtt ttctgtcgca
     2941 gcccgccgga gcctgacaag tatcgggatt ctgacgcgcc ccggtttgcg gacaggaaca
     3001 cgtcaactgg aaaagtttcc ctgtcgaatg tatgatcccg tcaagtaatc cgaggtctgg
     3061 caatgtcaaa tcgttttttc atcagtctgc ttgcgccttt tgccgccttc tcgatgacca
     3121 tgacggggtg cggtcaggag tcgtcgcccg atgacctgaa cggcgcgatg gatgtcgggt
     3181 atgaactcca ggagccggat tcaattgatc cggattccgg cgaccaggat gtcccggaca
     3241 acctctgccg gcttgatgcc ctttgcgacg tgcaggatga cgtcggaccg ggatcgccgg
     3301 gaaccttctg cgaagagaat tccgactgcg aatacggatt ctgcctgccg tacatttacg
     3361 gaaatatatg cagttcgccg tgttccagtt cttgtccatt cccgggctgg acgtgcgagg
     3421 ccctggtact tgcgggcggc gatactacat acgtctgtct tcccccggcg accgggcttt
     3481 gcaagccctg catgaccgat gcggcctgcc gccttgtcga gggcgaggag tcggcaaggt
     3541 gcgtttccta cggagaggag gccagttttt gcgcgaccga ctgcaccgcg aatccggagt
     3601 cctgcctcga gggttttgaa tgccgggaag tcgaggccgg cttcagcgca tgcgtgaagg
     3661 aggacggtct ttgcccgtgc gtgtcgccgg ttgcgggcat ggagtccagg tgcagccgtt
     3721 cgaatcagtc gggaacctgc tttggcagtc gattctgcac aagaaccggt aatgagttcg
     3781 cctggggcgc gtgttcggtt ggcgagcctt cccaggaggt gtgcaacagg attgacgacg
     3841 actgcaatgg cgcgaccgac gacatgggta ccgaggtctg cggaaccggc gtgtgcgcgc
     3901 acatggttga aaagtgtgtg gatggccagg cattcctgtg cgacccgtac gaaggaagcc
     3961 aggatgaagt ctgcaacggg ctggacgaca actgcaacgg cacgaccgac gagaactggc
     4021 cggacaaggg cgcggcatgc gacggcgggg atactgatgt ctgcgccaac ggaacctggg
     4081 gctgtgccga ggacggcatg gggctgacat gtgaaggtga catcgcttcc gacgtggaat
     4141 tgtgcaacgg ggtcgatgac gactgcgacg gcaagaccga cgagcccgag gatctcggca
     4201 ccatcgagtg cggcctgggt gcgtgccatc gggttcttcc cgcctgcacg tcaggcgttc
     4261 cgactgaatg tgatccgatg ctgggggccg tttcgccgga tatccctgac atcgacatgg
     4321 atgatgtgaa ctgcgacggt gttgacggcg acgcgccgtc agccgtattc gtcgata
//
LOCUS       DAOPPA010000271         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22041_length_4336_cov_3.543798, whole genome shotgun sequence.
ACCESSION   DAOPPA010000271 DAOPPA010000000
VERSION     DAOPPA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22041_length_4336_cov_3.543798"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKH54_08875"
     CDS             complement(<1..439)
                     /locus_tag="PKH54_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00048.1"
                     /translation="MKQNLKTVLVWLLLIGVFVAIVETVDRKPASSEMVFSQFITAAL
                     PDPTTPDGPLPSSLVKEVTITGQKIEGRMKDGKEFKTFGDVSEYQKDLVGRGISITYK
                     DETGGIWLQLLTSWLPMLFFLFIFLFFIRQSQSGGGKLFSFGKS"
     gene            complement(487..1167)
                     /locus_tag="PKH54_08880"
     CDS             complement(487..1167)
                     /locus_tag="PKH54_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD00049.1"
                     /translation="MEVSEALRSRRSTRAFEPDGLLLGREVLEDLIFEATRAPSEFNL
                     QPWRFLVVRDRERKQALYDCCFKQEKIRQASALIVVCGDTRGWERADKAAEELIETGY
                     FDPGPAAQPGQQSIQSAQNDARALAERIKNTYLRDERARIAMAYRNPALVAMNLMLVA
                     TERGIASCPIAGFSEPQLRRAFEIPDRFVPVVIIALGMPSLAMGRQPVKPRRPLFEVV
                     YHEDMASW"
     gene            complement(1329..2666)
                     /locus_tag="PKH54_08885"
     CDS             complement(1329..2666)
                     /locus_tag="PKH54_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOD00050.1"
                     /translation="MKKNFVLDTNVLLHDPAAMTGFADNNVCVPIYVIEEIDNFKKEL
                     SELGRNAREISRNLDKYRALGNIGQGVELESGGSVRVLFSSRHDVMDSEIIHRDINLS
                     HVMDRLIMGTALELKMAEPDVPLVFVTKDTNLRIRAEAMGLTAVDYDKENKDVSELYT
                     GTGELLLEGDMIAEFFRVGHVAIPAGVKNLFQNQYILIRNATNPSQSALARASTKDGR
                     FHKTVEVRDSVWGIKPRNKEQHFAFDALLRDDISLVTLIGKAGTGKTLLAIAAGLQKV
                     TDEQVFTRMLVSRPIFPMGRDIGYLPGDVEEKLNPWMQPIHDNLELLMGFNKQDRRSN
                     RSPRELMDMNMIKVEPLTYIRGRSIPNQYMIVDEAQNLTPHEVKTILTRVGEGTKIVL
                     TGDPYQIDNPYVDSVNNGLVYIAEKFKDSEIAATVTLTKGERSKLAELAANLL"
     gene            complement(2758..>4336)
                     /locus_tag="PKH54_08890"
     CDS             complement(2758..>4336)
                     /locus_tag="PKH54_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOD00051.1"
                     /translation="AIVPQEVGGQGLPVLMGQVIHEFFLGSNISFALLGGLSEGVIDL
                     IHHFGNEALKDMYLPGLLEQGWAGTMCLTEDNAGSDVGASRAVARKLDDGTFLIEGTK
                     IFITGGDQDMSKNIVHAVLARVEGDAPGTKGLSLFMVPKFRVGADGKMLAGPVPNDVR
                     CARIEHKMGIKASPTCVMNFGEKGECIGHLLGEQGAGMKQMFHMMNEARIMIGCQGLG
                     LAAAAYEKSRAYAMERLQGSDIEHMKDPKAPKVAIINHPDVRRMLMFQKAAVEAMRGL
                     LYMTAMYEDLAREAQTDEEKAKYRGWVELLTPICKSWCSDLGFKSCDLGLQCLGGYGY
                     TNEYPLEQFVRDARIGPIYEGTNGIQAMDFIGRKVPMNNMKNFQGFMKEIHAFVAENS
                     GHPVLGDLCGKLKVAANQVAEAAMKLLMAAMSDLSYTLSHAYMIQDAFGEMVGAWMLL
                     KQAVVASAKLDAMYAEKGLVMDADKLNLVKSNPDAAYYFGKIKAAEFYCREILPFVAA
                     RVEIMKSGDRSAVEMVF"
BASE COUNT          868 a         1397 c         1133 g          938 t
ORIGIN      
        1 tgctctttcc gaacgaaaac agctttccgc cgccggactg ggactgacgg atgaagaaca
       61 ggaagataaa aagaaagaac agcatcggca gccagcttgt cagcaactgc aaccagatcc
      121 caccggtttc atccttgtat gtaattgaga tcccgcgccc gaccagatcc ttctggtatt
      181 cgctgacatc gccgaatgtc ttgaattcct tgccgtcctt catccggcct tcaatcttct
      241 ggccggttat cgtgacttcc ttcacaaggc tggaaggcaa tgggccgtcc ggcgtggtcg
      301 gatccggcaa ggcggctgtg ataaactgcg agaacaccat ctcgctgctg gccggcttgc
      361 gatcgaccgt ttccacgatt gcaacgaata caccaatcag aagaagccac accagcacgg
      421 tcttaaggtt ctgtttcacc aatccaccat tattcagcct gcgcggtaat tacgcggcaa
      481 ggcaggctac caggacgcca tgtcctcgtg ataaacgacc tcgaaaagcg ggcgccgcgg
      541 cttcacaggc tgccttccca tcgccagcga cggcatcccc agggcaatta tcacgactgg
      601 aacaaagcga tccggaatct caaaagccct tctcaactgg ggttcggaaa acccggcgat
      661 cgggcacgat gcgatgccac gctcggttgc aacaagcatc aggttcatcg cgaccagggc
      721 cggatttctg tacgccatgg caattcgggc gcgctcgtca cgaagataag tattcttgat
      781 ccgttccgcc agtgcccttg cgtcgttctg agcggactga atcgactgtt gacccggctg
      841 cgccgccgga cccggatcga agtaccctgt ttcaatcaat tcttccgcgg ccttgtccgc
      901 gcgctcccac ccgcgggtat cgccgcagac gacgatcaga gcacttgcct ggcgaatctt
      961 ttcctgcttg aaacagcaat cataaagggc ctgcttgcgc tcgcgatcgc gaacaaccag
     1021 gaaccgccag ggctgcaggt tgaattccga aggcgctcgc gtcgcctcga aaatcaaatc
     1081 ctccaggacc tcgcggccaa gcagcaggcc gtccggctcg aacgccctgg ttgatcgacg
     1141 actccgcagg gcctctgata cttccatcag gttatctcca catcttccgc caatttccct
     1201 gtacgggcgg gttacatccg cccgccgcgg ttccgcacgt ccttccctgt acgggcgggt
     1261 tacatccgcg ggccgcggtt ccgcacgttc cgtacgggcg aatgaaaatt cgcccgcccg
     1321 cacccgtctt acaacagatt cgccgccagt tccgccagct tcgaccgctc gcccttggtc
     1381 agggtaaccg tcgcggctat ctcggaatcc ttaaactttt cggcgatata caccagaccg
     1441 ttattgaccg aatcgacgta cggattgtcg atctggtacg ggtcgcccgt gagcacgatc
     1501 ttcgtgccct cgccaacgcg cgtcaggatt gtcttcacct cgtgcggcgt caggttctgc
     1561 gcctcatcga cgatcatgta ctggttcgga atcgagcgcc cgcgaatgta cgtcagcggc
     1621 tcgaccttga tcatattcat gtccatcaat tcgcggggcg acctgttcga gcgccgatcc
     1681 tgcttgttga atcccatcag cagttcaagg ttgtcgtgaa tcggctgcat ccacggattc
     1741 agtttctcct cgacgtcacc cggcaggtac ccgatgtcgc gccccatcgg gaaaatcggc
     1801 ctggaaacca gcatcctggt aaagacctgt tcatccgtga ccttctgcag gccggccgca
     1861 atcgccagca gcgtcttgcc ggtgccggcc ttcccgatca gcgtcaccag actgatgtcg
     1921 tcacgcagaa gcgcgtcaaa tgcgaaatgc tgttccttgt tccttggctt gataccccag
     1981 acggaatcgc gaacctcaac cgtcttgtga aagcgtccgt ccttggtcga tgccctggct
     2041 agcgcgctct gggaaggatt cgtcgcattg cgaatcagga tgtactggtt ctggaacagg
     2101 ttcttcacgc cggccggaat cgccacatgc cccacgcgga agaactcggc gatcatgtcg
     2161 ccttcaagca gaagttcgcc ggttcccgtg taaagctccg atacgtcctt gttttccttg
     2221 tcgtaatcga cagccgtcag gcccatcgcc tcggcgcgta tgcgcaggtt cgtgtccttg
     2281 gtcacgaaca ccagaggcac gtccggctcg gccatcttca gttccagcgc cgtgcccatt
     2341 atcaacctgt ccatcacgtg gctcaggttg atgtccctgt ggatgatttc ggaatccatc
     2401 acgtcgtgac gcgacgaaaa cagaacccgg acgcttccac cagattccag ttcgacgccc
     2461 tgtccgatgt ttccaagcgc acggtacttg tccaggttcc tggaaatctc tcgtgcgtta
     2521 cggcccaact cggacaattc cttcttgaaa ttgtcgattt cctcgatgac gtaaatcggc
     2581 acgcacacat tgttgtcggc aaaccccgtc atggccgccg gatcgtgcag cagaacgttg
     2641 gtatcaagca caaaattctt tttcatcgtg acccccggca gtgccagccc ttccggcgaa
     2701 agcttttcgc ggtgaaagat tatatctgtt gacggaaaaa ggctatcgaa gagaggctca
     2761 gaacaccatc tcaaccgcgc tgcggtcgcc cgacttcatg atttcgacgc gtgcggcgac
     2821 gaagggaagg atctccctgc aatagaactc ggcggccttt atcttaccga agtagtacgc
     2881 cgcgtcggga ttggacttca ccaggttcag cttgtcggcg tccatcacca ggcccttttc
     2941 agcatacatg gcatccagct tcgcgcttgc cacgacggcc tgcttcagca gcatccaggc
     3001 gccgaccatc tcgccgaagg catcctggat catgtacgca tgcgaaagcg tgtacgacag
     3061 gtcggacatg gcggccatca acagcttcat cgccgcttcc gccacctggt ttgcagcgac
     3121 cttcaacttg ccgcacagat cgccaagcac cggatggccg gaattctccg cgacaaacgc
     3181 gtggatttcc ttcatgaatc cctggaagtt cttcatgttg ttcatcggaa ccttgcggcc
     3241 gatgaagtcc atcgcctgga tgccgttggt gccttcgtaa atcggcccga tcctggcgtc
     3301 acgcacaaac tgctccagcg ggtattcatt ggtatagccg tatccgccca ggcactgcaa
     3361 ccccaggtcg cacgacttga atccaagatc cgagcaccac gacttgcaga tcggcgtgag
     3421 caattcgacc catccgcgat acttcgcctt ttcctcgtcg gtctgcgcct cgcgcgcaag
     3481 atcctcgtac atcgcggtca tgtaaagcag gccgcgcatc gcctcgacgg ccgccttctg
     3541 gaacatcagc atgcgccgga cgtccggatg gttgatgatc gcgaccttcg gggccttcgg
     3601 atccttcatg tgctcgatgt cgctgccctg cagacgttcc atcgcgtacg ccctcgactt
     3661 ctcataggcc gccgccgcca gtccaaggcc ctggcagccg atcatgatgc gggcttcgtt
     3721 catcatgtga aacatctgct tcatgccggc gccctgctcg ccaagcaggt ggccgatgca
     3781 ttcacccttt tcaccgaagt tcataacgca tgtcggggac gccttgatgc ccatcttatg
     3841 ctcgatgcgc gcgcacctga cgtcgttcgg cacaggacct gccagcatct tgccgtccgc
     3901 accgacgcgg aacttcggaa ccatgaacag cgacagcccc ttcgttccgg gggcgtcgcc
     3961 ttcaaccctt gccaggaccg catggacgat gttcttcgac atgtcctggt cgccgccggt
     4021 gataaatatc tttgtgcctt cgatcaggaa ggtgccgtcg tccagcttcc gtgcaacggc
     4081 acggcttgcg ccgacgtccg agcccgcgtt gtcctcggtc aggcacatgg ttccggccca
     4141 gccctgctcc agcaggcccg gcagatacat gtccttcagc gcttcattgc cgaaatggtg
     4201 gatcaggtca atcacgccct cggacaaccc gccaagcagc gcgaaggaga tgttcgaacc
     4261 caggaaaaac tcgtgaatga cctgtcccat caggacgggc agtccctggc caccaacttc
     4321 ctgcggaaca atggcc
//
LOCUS       DAOPPA010000272         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22066_length_4332_cov_3.801263, whole genome shotgun sequence.
ACCESSION   DAOPPA010000272 DAOPPA010000000
VERSION     DAOPPA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22066_length_4332_cov_3.801263"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKH54_08895"
     CDS             complement(<1..926)
                     /locus_tag="PKH54_08895"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD00052.1"
                     /translation="MNKMISNALTALRQLLDAKPGHTILIVSDESTRDIADAWHKAAI
                     RLECRVINFVLPEARRPLQDIPRALIEMMPDADIAITAFSGLMDEAPFRLSLLSELSR
                     HVRRIGHSPGITRYMMTSGGMTADYGSLDATARDLINRLEGAVCLKVTAPGGTEMTID
                     VTDRPFSTDTAIPDGSAGNLPCGEIWCAPVESNANGVLVCDGSIGDLGRVPAPVTLVV
                     NNGKVTSVMCQDPDFRAEVETLLEYDAGAGIIGEFGIGINPAASVIGNLLEDEKAAGT
                     AHIAFGNNMGMKGGRNASRTHQDFLFTDPTVTV"
     gene            complement(1018..3498)
                     /gene="lon"
                     /locus_tag="PKH54_08900"
     CDS             complement(1018..3498)
                     /gene="lon"
                     /locus_tag="PKH54_08900"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD00053.1"
                     /translation="MSKADEFDPGKIEALPILPLRNAVLFPGLVMPLVIGREKTLRLL
                     DAVADTGRIIGVITQKDKDIADPGPDDLYVTGTTARILRVVKERDQGVDIVVQGIQRF
                     RTVEYGKTEPFLTADVQLLPDHNTDDAEVEALTNSLKTLTSEVFSLMPEMPSTAAEMV
                     ENVNSPSRLVYLVTSNLSISTEDKIRVLEIEDIADALRETLKTLSHHLEVLRVTQRIN
                     TEVKGELSKSQREYLLRQQIKAIQKELGDIEDDSDVLDDIGEKLRNTKLTDEVRKVAE
                     KELRRLRSIQPSSPEYTVARTYLDWITELPWGVETEDNLDVEHAREVLDADHYDLVKI
                     KRRILEHLAVSKLRNNMRGPILCLVGPPGVGKTSLGQSVARALGRKFIRVSLGGVRDE
                     AEIRGHRRTYIGALPGKIIQSLKRAGSSNPVFMMDEIDKLGRDWRGDPTSALLEVLDP
                     EQNHSFMDHYMDVPFDLTGILFITTANVTDTIPAPLLDRMELLELPGYTPEEKLAIAR
                     RHLLPKQIKEHGLGDHSIDLSDEMILYVIEHYSREAGVRNLERNVASVCRGIAVRIVE
                     GKWEGKPVDREMINSFLGPEQFQVETAERTESPGVATGLAWTATGGEILFVEATRMAG
                     RGKLKLTGQLGDVMKESTQIALSFLEANAEKFGIKPPQFADIDIHVHVPAGAIPKDGP
                     SAGVTMFTALFSLMTGRKVRSDTAMTGEITLRGLVLPVGGIKNKVLAAHRGGIKRIIL
                     PERNRKDLEEIPDQVRREVEFIFVGRIDDLLNHVIDLSDGRPLFFTDQPAVPVEALPA
                     ARHDEEDDDVPRQVDDRAKEEDIPRPWL"
     gene            complement(3608..>4332)
                     /locus_tag="PKH54_08905"
     CDS             complement(3608..>4332)
                     /locus_tag="PKH54_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD00054.1"
                     /translation="AGMPQRAEVFPVSPMSDRPVPRQIAIPAGTKPARDASASKGSKD
                     APVVFIEISDFQCPVCKRAHNELAPLLTDFPDQVRYVFKHNPLEMHRDALNASAASLA
                     AGRQGRFWEYADALFADQRALSEPDLMAIAQRLKLDMKRFKADYEDPALRQRAKTEGD
                     IAMAIGARGTPAFLVNGRLQVGWASYAAVRQQVEQELAAMAALTSTGKTLRQAREERV
                     RTMLPEAEKLLNTPLGIELTER"
BASE COUNT          821 a         1251 c         1346 g          914 t
ORIGIN      
        1 accgtgacag tcgggtccgt gaaaaggaag tcctggtgcg tccgtgaggc gttacggccg
       61 cctttcatgc ccatgttgtt gccgaaagcg atgtgggcgg tgccggcggc cttttcatcc
      121 tcaagcaggt tcccgatgac cgaggcggcg ggattgattc cgattccgaa ttcaccgatg
      181 attccggcgc cggcgtcata ttccagcaag gtttcgactt cggcccggaa atcgggatcc
      241 tggcacatga ccgaagtgac cttcccattg ttcactacaa gcgtcactgg tgccggaacc
      301 cgaccaagat ccccgatgga accatcgcat acaaggacgc cattggcgtt ggactcgacc
      361 ggcgcgcacc atatctcgcc gcatggaagg ttgccggcgg agccgtccgg aatcgccgta
      421 tccgtcgaga atgggcgatc cgttacgtca atcgtcattt ccgtcccacc cggggccgtg
      481 accttcaggc agacagcgcc ttcgagccgg ttgataaggt cacgcgcggt cgcatcgagc
      541 gacccgtagt ccgccgtcat gcccccggac gtcatcatgt acctggtaat ccccggcgaa
      601 tgtccgatcc tgcggacatg acgcgacagt tcggaaagca gcgacagccg gaatggcgcc
      661 tcgtccataa ggccggaaaa tgcggtgatg gcgatatctg cgtcgggcat catttcgatc
      721 agtgcgcgcg gaatgtcctg caacgggcgg cgggcctcgg ggaggacgaa attgatcacg
      781 cggcattcaa gtcggatcgc agccttgtgc caggcatcgg cgatgtccct ggtcgattcg
      841 tcggaaacga tcaggatcgt atggccgggc tttgcgtcaa gaagctgcct cagggcggtc
      901 agtgcgtttg aaatcatttt gttcatgaca acccccgggc aaatgacagg gaaacggtta
      961 atcccgaatc atgacgatag caccagggat atggcacaaa caaccagaaa caggggatca
     1021 aagccacgga cgcgggatat cctcttcttt tgcccggtcg tccacctgtc gcggcacgtc
     1081 atcgtcttct tcatcgtgcc gggctgcggg aagcgcctcg accggaacgg caggctggtc
     1141 ggtaaagaac agcgggcgac cgtctgaaag gtcgattacg tggttcagca gatcgtcgat
     1201 acgaccgaca aatatgaact cgacctcgcg gcggacctga tccgggatct cctcgagatc
     1261 cttgcggttc cgttccggca ggatgatccg tttgataccg ccacgatggg ccgccagtac
     1321 cttgttcttg atgccgccaa cgggcagaac cagtccacgc agggtgattt cgccggtcat
     1381 cgcggtatcg ctgcgaacct tgcggccggt catcagcgag aagagcgccg tgaacatcgt
     1441 gacgccggcg gacgggccgt ccttcgggat ggcaccggcc ggcacgtgaa catgaatgtc
     1501 gatgtcagcg aactggggcg gcttgatccc gaacttttcg gcgttggcct ccaggaagga
     1561 cagggcgatc tgggtcgatt ccttcatgac gtcgccaagc tgtccggtaa gtttcagctt
     1621 gccgcgtccg gccatccttg tggcttcgac gaacagtatc tcgccgccgg tggcggtcca
     1681 ggcgagtccg gttgcgacgc cgggggattc ggttctttcc gcggtctcga cctggaactg
     1741 ctcgggcccg aggaatgaat tgatcatttc acggtcaacc ggctttcctt cccatttgcc
     1801 ctcgacaatg cgcaccgcga tgccgcgaca gacagaggcc acgtttcgtt ccaggttacg
     1861 gacgccggcc tcgcgggagt agtgctcgat tacgtacaga atcatctcgt cggacaggtc
     1921 gattgagtgg tcgccaagcc cgtgttcctt gatctgcttt ggcaacaggt gccgacgggc
     1981 gattgccagc ttttcctcgg gtgtgtaacc cggcagttca aggagttcca tgcgatccag
     2041 cagcggagcg gggatagtgt cggtaacgtt ggctgtggtt atgaacagga taccggtcag
     2101 atcgaaagga acgtccatgt aatgatccat gaacgaatga ttctgttccg gatcgaggac
     2161 ttcaagaagg gcactggtgg ggtcgccgcg ccagtcgcgt cccaacttgt caatctcgtc
     2221 catcatgaag accgggttcg aactgccggc gcgcttcagg gactggatga ttttgccggg
     2281 cagcgcgccg atgtaggtgc gccgatgtcc gcgaatttcg gcctcgtcac gcacgccgcc
     2341 aaggctgacc cggatgaact tgcggccaag tgcgcgggcg acggactggc cgagagaagt
     2401 cttgccgacg ccggggggcc cgaccaggca gagtatgggc ccgcgcatgt tgttgcgaag
     2461 cttcgacacg gcaaggtgct caaggatacg acgctttatc ttgaccaggt cgtagtgatc
     2521 ggcgtccagc acttcccgcg cgtgttcgac gtcaaggttg tcctcggtct ccacgcccca
     2581 cggaagctcg gtaatccagt caagataggt tcgcgcgacc gtatattccg gtgaagaagg
     2641 ctgaatcgag cgaagccgac gcagttcctt ctccgcgacc ttgcggactt catcggtcag
     2701 cttcgtgttg cgaagctttt ccccgatgtc gtcaagaacg tcgctgtcgt cctcgatgtc
     2761 gcccagttcc ttctggattg ccttgatctg ctggcgaagc aggtattccc gctggctctt
     2821 gctgagttcg cccttgacct cggtattgat gcgctgggtc acgcgcagca cttccaggtg
     2881 atgggagagc gtcttaaggg tttcccgaag cgcgtccgcg atgtcctcga tttccagaac
     2941 cctgatcttg tcctcggtgc tgatggacag gttcgaggtg accagataga ccagccgtga
     3001 tggcgagttg acgttttcaa ccatctcggc ggccgtcgaa ggcatctcgg gcatcaggct
     3061 gaagacctcg gaagtcaggg tcttcaggct gtttgtcagc gcctcgacct ctgcatcgtc
     3121 ggtgttgtga tccggcagga gttggacgtc cgccgtcagg aagggctcgg tcttgccgta
     3181 ttcgacggtg cggaatctct ggattccctg aaccacgata tcgacaccct gatcgcgctc
     3241 cttaacaacc ctgagtatcc gggcggtggt tccggtaacg taaagatcgt ccggtccggg
     3301 atccgcgatg tccttgtcct tctgtgtgat gacgccgatg atgcgaccgg tatcggccac
     3361 agcgtccaaa aggcgcaggg tcttttcccg cccgataaca aggggcatca caagacccgg
     3421 gaacagaacg gcgttgcgca atggaagtat cggcaatgct tcaatctttc cggggtcgaa
     3481 ttcgtccgct ttgctcattg tcgccatcca tcaattcgcg gattgaatcc gcgttggcca
     3541 atcagaacca ccggggcagc cggaggtcag ggggaataat tgtcacgaac gggaaaaggt
     3601 caagtttcta tcgttcggtc aattcgattc caaggggagt attcaacagc ttttctgcct
     3661 cgggaagcat cgtccggacg cgttcttcgc gggcctggcg aagcgtcttg ccggttgaag
     3721 tcagcgcggc cattgccgcc agttcctgtt cgacctgctg cctgacggcc gcatagctgg
     3781 cccagccgac ctgcaggcgt ccgttgacca ggaaggccgg ggttccgcgg gccccgattg
     3841 ccatggcgat atcgccctcg gtttttgcgc gctggcgaag cgccggatct tcgtagtcgg
     3901 ccttgaaacg cttcatgtca agcttaagcc gctgggcaat cgccatcaga tccggttccg
     3961 aaagtgcgcg ctggtcggcg aagagcgcgt cggcatattc ccagaaacgt ccctggcgac
     4021 cggccgcgag cgaagcggcg gaagcgttca gcgcgtctcg gtgcatctcg agcgggttgt
     4081 gtttgaagac atagcgcacc tgatccggga aatcggtcag gagcggggca agctcgttgt
     4141 gggcgcgctt gcagaccggg cactggaagt cggatatttc aatgaaaacg accggagcat
     4201 ccttgcttcc cttcgaggcg cttgcgtcgc gggccggctt cgttccggca ggaatcgcga
     4261 tctggcgcgg aacaggccga tcggacatgg gagaaacggg gaagacttcg gcccgctgtg
     4321 gcattccggc ct
//
LOCUS       DAOPPA010000273         4325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22122_length_4325_cov_4.370726, whole genome shotgun sequence.
ACCESSION   DAOPPA010000273 DAOPPA010000000
VERSION     DAOPPA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22122_length_4325_cov_4.370726"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..3380
                     /locus_tag="PKH54_08910"
     CDS             36..3380
                     /locus_tag="PKH54_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HOD00055.1"
                     /translation="MRLLCAAAMVAFGAWLVGCGGGGGFDTGPADEGALVDIDVADVP
                     GEVVDAGEDAGDAGGDSGFEWPAGMSPVVEPADPVLAGGLPAATAFDASNRPMLDLSG
                     DWQFKFDDADAGVTDGWFATAFDRKKWIKAPVPGTWDMDVEGGFDRQTVAWYAREFSW
                     DDESPFVRLRFDGVFREARVWLNGVELGGDDLPYLPFAFDVTKLLRRDSANVLVVRVD
                     NRLGRQTLPCDTSLTPGLLGWFPYGGIPRPVFVEGSRSTSVARLAVKTSTGDVHWAVT
                     ADVLLQQAAGISGTARLSAAVKKGDVILIQWGPAELPADVSRLSLEGLVALPDLWDPE
                     HPDRTCRMDVVISQDGVDETVSVDFAFRTLEAVSGRLLLNGSDRFLHGMNRHEDLPGR
                     GPVFDADAMAADVAAMKDLGVDFVRPAHYPNDVRTLRALEAAGMLLAQEIPVYQLDDR
                     QLADPVMLDRATRALRRMIHRDINRPAIGLWSIANEVWTFADTAPAFLRSLRETAREL
                     DPDRPVMAAVLSAPGVSFSDVDLGPGEVDVIGINEYHGWYFGLTTDLGDLLDAEHARF
                     PDKTLIVSEYGCDALRARHGYGQPGEEPVDDHSYTEEYQAWFHRQHLKQIAEREFVRG
                     VAPWVLADFRMQWSPTTGKPHPAPKTNLKGLMDAWRRPKAAYDVVKAAYAGPDMQPVP
                     VELAVPHEPDCGNGAVEPPEACDGGTIPCADLGVSYASGTASCRPDCTGWDVSGCTRA
                     TPPELGWEVVKPALRDPARWGDARCNDGTPFAFRVRLSPTGSPDWVISLEGGGFCDDY
                     AVPCIRPIEKTTTLPDQDGTWLVPHDDNGGIFDVDVADNPVWHDANWVLGPYCTSDFW
                     LGTRTSRIWSLGDAENGWYFAGRLNVQALLETLVRHYGLADVADDGGPKVLFSGTSSG
                     ALGTMATADLAVAMLPNTAAAGRLKVMVDSGWLVHDWDDPEARLVMAQVSDLEAVRIA
                     HDHFQARLNPICESARIRDGGHAGDCLMGLFAFPYLVGEPPDGLGLPVLFQQSLADTA
                     FSGFHNHDDDMDFQVAYGLRQQADALEADPGDPLMAWWFLGRSAYHDICRKTDRWTIG
                     EPGNTFRDLLERFWAGGKPERVIWNP"
     gene            complement(3483..3962)
                     /locus_tag="PKH54_08915"
     CDS             complement(3483..3962)
                     /locus_tag="PKH54_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD00056.1"
                     /translation="MYRIILADYADPTHRDAVVEILDAYARDPMGGGEPLPEFTRRHL
                     LDEMARRPNMFSLLAFIDDRPVGLANCIEGFSTFAARPLLNIHDLAVLPGYRGRGVGR
                     ALLAEIESIARSRGCCKVTLEVLQGNHAARTAYLNFGFKPYMLGDTLGNAEFWHKHL"
     gene            4089..>4325
                     /locus_tag="PKH54_08920"
     CDS             4089..>4325
                     /locus_tag="PKH54_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00057.1"
                     /translation="MDAGVWVFTRQRPAGGLLVSFLFVLVMTTGCGGGTVDPCDGIDC
                     DGHGVCAIRDGLATCLCDTGYATSGTTCVTDVCSP"
BASE COUNT          699 a         1285 c         1531 g          810 t
ORIGIN      
        1 gatgggatcg ggaatacatg gggaagacac ggcatatgcg attgctttgt gcggccgcga
       61 tggttgcctt cggcgcctgg ctggtcgggt gcggcggtgg cggtggattc gatacggggc
      121 ccgccgatga aggtgctttg gtcgatatcg acgtggcgga tgttccgggt gaagtcgtcg
      181 atgcgggtga agatgccggc gatgcgggtg gtgattccgg gttcgagtgg ccggctggca
      241 tgtccccggt cgtggaaccg gcagatcctg tcctggcggg agggctgccg gccgcgaccg
      301 ccttcgatgc ttccaatcgg ccgatgctgg atctgtcggg cgactggcag ttcaagttcg
      361 acgatgccga cgcgggcgtg accgacggct ggttcgcgac tgcgttcgat cggaagaagt
      421 ggatcaaggc gccggttccg ggcacctggg acatggatgt cgagggcggg ttcgaccggc
      481 agaccgtcgc ctggtatgcg cgcgagtttt cctgggatga cgaaagcccg tttgtccggc
      541 tgcgctttga tggcgtcttc cgcgaggccc gcgtctggct taacggcgtg gaactgggcg
      601 gcgacgacct gccttatctg ccgttcgcgt tcgacgtgac gaaactgttg cgccgcgact
      661 cggcgaacgt gctggtcgtg cgcgttgata accggcttgg ccgccagacg ctgccgtgcg
      721 atacctccct gaccccgggg cttctgggct ggttcccgta cggcgggatc ccccggccgg
      781 ttttcgtcga gggttccaga tccacgtcgg tggcaaggct ggcggtcaag acatcgaccg
      841 gcgacgttca ctgggccgtg accgcggacg tccttctgca gcaggccgcc gggatttccg
      901 ggacggcacg cctgtcggcc gcggtgaaaa agggtgacgt gatcctgatt cagtgggggc
      961 cggcggaact gcccgccgac gtgtcgcggc tgtcgcttga aggcctggtc gccctgccgg
     1021 atctctggga tccggagcat cccgaccgga cctgccggat ggacgtcgtg atatcgcagg
     1081 acggcgtcga tgaaacggta tccgtcgatt ttgcgttcag gacgcttgaa gccgtttccg
     1141 gccgcctgct gctgaacggc agtgaccgtt ttctgcacgg aatgaaccgg cacgaggatc
     1201 ttcccggcag ggggccggtg ttcgacgcgg acgcgatggc agcagatgtc gcggcgatga
     1261 aggatctggg tgtcgatttt gtgcggccgg cacattatcc gaacgacgtc aggacgttgc
     1321 gggcgcttga agcggcgggg atgctgctgg cccaggaaat cccggtctat cagcttgacg
     1381 accggcaact ggccgaccct gtcatgctgg acagggcgac gcgggcgctg cgccgcatga
     1441 tccacaggga tatcaaccgg ccggccatcg gcctgtggag cattgcaaat gaagtctgga
     1501 cgttcgccga caccgccccg gcgttcctgc gcagcctgcg tgaaacagcg cgcgagcttg
     1561 acccggaccg gcccgtgatg gcggccgtgc tgtccgcccc gggcgtgtcg ttttcggacg
     1621 ttgatctggg gccgggcgaa gtcgatgtga tcggcatcaa cgagtatcac ggatggtact
     1681 tcggcctgac gaccgatctt ggggatctgc tggacgcgga gcacgcgcgg ttcccggaca
     1741 agacactgat cgtcagcgag tacggctgtg atgccttgcg ggcgcggcac ggctacgggc
     1801 agcctggcga ggaaccggtc gatgaccact cgtacaccga ggaataccag gcgtggtttc
     1861 atcggcagca cctgaaacag attgccgaaa gggaattcgt ccgcggcgtc gccccgtggg
     1921 tgctggccga tttcaggatg cagtggagtc cgacgaccgg caagccgcat cccgcgccaa
     1981 agacgaacct gaagggactg atggacgcgt ggcggcgccc gaaggcggcg tacgacgtgg
     2041 ttaaggccgc atacgcaggt cccgacatgc agccggtgcc ggtcgaactt gccgtgccgc
     2101 atgaacccga ctgcggaaac ggcgcggtcg agccgcccga ggcctgtgac ggcggaacca
     2161 tcccgtgcgc tgatctgggc gtttcttatg cgtccgggac tgcttcgtgc cgacccgact
     2221 gcaccggatg ggacgtttcc ggctgcacgc gtgccacgcc ccccgaactg gggtgggagg
     2281 tcgtgaagcc cgcgctgcgg gatccggcgc gatggggcga cgccaggtgc aacgacggga
     2341 cgccgttcgc gttcagggtc cgcctttcac cgacagggtc gccggactgg gtgatcagcc
     2401 tggaaggcgg cggcttctgc gacgactatg ccgttccgtg catccgtccg attgaaaaga
     2461 ccacgacgtt gccggatcag gacggcacat ggctggttcc ccacgacgac aacggcggga
     2521 tttttgacgt cgatgtcgcc gacaacccgg tctggcacga cgcgaactgg gttctcggac
     2581 cttactgcac cagcgatttc tggcttggga cgcggacttc aaggatatgg tcgctggggg
     2641 atgccgaaaa cggctggtac ttcgccggcc gcctgaacgt gcaggcgctg ctggagacgc
     2701 tggtccggca ttacggcctg gccgatgtcg cggatgatgg cgggccgaag gtgctgtttt
     2761 cgggaacgtc gtcaggcgcc ctggggacga tggccacggc cgacctggcg gtcgcgatgc
     2821 tgccgaacac ggctgcggcc ggtcgcctga aggtgatggt cgattcgggc tggctggtgc
     2881 acgactggga cgacccggaa gcgcggctgg tgatggccca ggtgtcggat ctggaagccg
     2941 tgcggatcgc ccatgaccac ttccaggccc gcttgaaccc gatctgcgag tcggcgcgaa
     3001 tccgggacgg cgggcatgcg ggcgactgcc tgatgggcct gttcgcgttc ccgtaccttg
     3061 ttggcgaacc tcccgacggg ctgggcctgc cggtgctgtt ccagcagagc ctggcggaca
     3121 cggcattctc gggtttccac aaccacgacg atgacatgga tttccaggtc gcgtacgggc
     3181 ttcggcagca ggccgacgcg ctggaggccg accccgggga tccgctcatg gcatggtggt
     3241 tcctgggccg ctccgcatac cacgatatct gccgcaagac cgaccgctgg acgatcggcg
     3301 agccgggaaa cactttccgg gatcttcttg aacgcttctg ggcgggcggc aaacccgagc
     3361 gggtaatctg gaacccgtga cagcgcgccg gcgacgtcgg gccagaggca tctgaattac
     3421 atgtgtggcc gccggaattc tgcgtgtcgc cacaggtgct tgtgccagaa ttcggcgtgt
     3481 tattacaggt gcttgtgcca gaattcggcg tttcccagcg tatcgcccag catgtatggc
     3541 ttgaatccga aattcaggta ggcggtgcgc gccgcgtggt tgccctgcag cacttcaagg
     3601 gtcaccttgc agcagccgcg tgaccgggcg atcgactcga tttcggccag cagcgcgcgg
     3661 ccgacgccgc ggccccgata acctggaagc accgcgaggt cgtggatgtt cagcaggggg
     3721 cgcgccgcga acgttgaaaa gccctcgatg cagtttgcca ggccgaccgg ccggtcgtcg
     3781 atgaaagcca gcagcgaaaa catgttcggc cggcgggcca tttcatccag aaggtgccgg
     3841 cgcgtgaact cgggcagcgg ctcgccgccg cccatcgggt cgcgcgcgta agcgtccagg
     3901 atttcgacga ccgcatcgcg gtgcgtcggg tcggcgtaat cagccaggat gattctgtac
     3961 atttcgcggt ttcgtccgga ttcccgcccg attattgcat gaaaacaagg tctccaatgc
     4021 gctggacatg ccgcagggcg ttcggctaga ctgcctttaa tcaatgatcc gaccggagcg
     4081 gggggaccat ggacgcgggc gtttgggttt tcacacggca aaggccggct ggcggccttc
     4141 ttgtttcatt ccttttcgtt ctggtgatga ccacgggttg cggcggcgga acggtcgatc
     4201 cgtgcgatgg gatcgactgc gatggccacg gggtctgcgc gattcgcgac ggtctggcga
     4261 cgtgtctgtg cgacaccgga tacgccactt cgggaaccac ctgcgtcacc gatgtctgca
     4321 gcccc
//
LOCUS       DAOPPA010000274         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22207_length_4312_cov_4.006342, whole genome shotgun sequence.
ACCESSION   DAOPPA010000274 DAOPPA010000000
VERSION     DAOPPA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22207_length_4312_cov_4.006342"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKH54_08925"
     CDS             <1..945
                     /locus_tag="PKH54_08925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD00058.1"
                     /translation="ERLTRLTQLLDMPQYTAMVAAQRAVYGDHPYGRPSAGTPESVKA
                     ITIQDVRATWADWFQPKNAVLIVVGDIDRDTLARYLNETASAWKNDGEVPDARLAPSK
                     PVAGTVNVVDMPGAHQSVIIVGGPAPARRGTDVATLNVLNSTIGGKFVSRLNLNLRED
                     KGYTYGARSAFEMHGWGGMFQASASVATDVTGPATGEILRELNEIGSTRPVTPEELKY
                     SVDAIVNGYSQRFSSIEKVASELLETYLYGLGADDIELYPSQVATVTTDQVTAAARTH
                     LAPEYLSITIAGDAGKVVPQLRQILGDRPINIIKRTTP"
     gene            1346..3568
                     /locus_tag="PKH54_08930"
     CDS             1346..3568
                     /locus_tag="PKH54_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOD00059.1"
                     /translation="MNGKKIDLPVIGMSCAHCAATVEKTLVRDVPGVSSAVVNLATES
                     VAVTFDERTTSPQAMADAVARAGFKLVLPSLDDVETNARRQNERRIRRELLVGIVFAL
                     PLLVLSMGSDFGLLGGLSHAPWLGWIYLTLATPVQFITGASFYIGAYRAVRNRALDMD
                     VLVALGSTVAYVYSLVLLVTPGHEHLYFETSAMIVVLIKVGKMLEARSRAVAARSIKS
                     LLDSAPDTASVIGPDGQERPIAVAALSVGDLVMVRPGEKVPVDGTVVSGASAVDESMV
                     TGEPIPVDKVPGDIVFGGTMNAEGLLTVRATGVGADSLLARIGAMVARAQASKAPIQR
                     FADRVAAVFVPVILVTAAVTFTFWMLWTDGDIQLAVMRSIAVLVIACPCAMGLATPMA
                     VMVGMGRGARGGILFRDAATMEMLHKVTAIAFDKTGTLTQGRPEIAAWVSTPGWEQTG
                     LELAAGAESGSGHPLAQAVVRYARSLDVSVPPPSDFQATPGIGVRATVSEHAVQVGNP
                     RRLDGIAIPDWMADRMHGLESMGHSVLAVLVDGETVGLFFLADQLRPTAVGAVAEMRE
                     MGLNLSMLTGDNASAAARVAAAAGIAVHQSGMMPADKEAAIAELQQGGHRVAMVGDGI
                     NDTPALARADIGIAPGAGAAAAIETADVTLVGSDLTLVARAVKLSRATIRKIRQNLFW
                     AFFFNVLCVPIAAGVFHGVEWMPAMLRDLNPMLAATAMAFSDIIVIGNSLRLNHVRLP
                     "
     gene            complement(3884..>4312)
                     /locus_tag="PKH54_08935"
     CDS             complement(3884..>4312)
                     /locus_tag="PKH54_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOD00060.1"
                     /translation="QLIFARWAMVMFFFEKDLYTNPDNPELNKIWYDRVVELQGLVRP
                     AERTAADWAAKPHFTIAPVYYHNYLLGELFATMLRAKLSEIAAHTGPASELDFVGKAD
                     FGRFIIAQIFEPGMTQPWPEFVKTAFGKPFTPDAFVAEVQ"
BASE COUNT          781 a         1363 c         1338 g          830 t
ORIGIN      
        1 gagcgcctga cccgcctgac ccagcttctt gacatgccgc agtacactgc catggttgcg
       61 gcccagaggg ccgtgtatgg cgaccatccg tacgggcgtc cgtccgcggg aaccccggaa
      121 tccgtcaagg caattacgat acaggatgtc cgcgcgacct gggccgactg gttccagccg
      181 aagaatgcgg ttctgatcgt ggttggcgac atcgaccggg acaccctggc gcgttacctg
      241 aacgaaaccg cgtccgcgtg gaagaacgac ggggaagtcc ccgatgcccg ccttgcgccg
      301 tcgaaacctg ttgccggaac ggtcaacgtg gttgacatgc ctggcgccca tcagtcggtc
      361 atcatcgtcg gcggtcccgc gccggcacgc cgcggcactg acgtcgcgac gctgaatgtc
      421 ctgaattcaa cgatcggcgg caagttcgtt tcacgcttga acctgaacct gcgcgaggac
      481 aagggctaca cttatggcgc ccgttccgcg ttcgaaatgc acggctgggg cggcatgttc
      541 caggcctcgg cgtcagtcgc gaccgatgta accggccccg caacgggcga aattcttcgc
      601 gaactgaacg agatcggctc gacgcgtccg gtcaccccgg aggaattgaa gtattcagtc
      661 gacgcgatcg tgaacggtta cagccagcgg ttcagttcga tcgagaaggt ggcttcggag
      721 ctgcttgaaa cgtacctgta cggacttggc gccgacgata tcgaactcta tccgtcccag
      781 gtcgccacgg tcacgaccga tcaggtcacc gcagccgcca gaacccatct ggccccggaa
      841 tacctttcca tcaccatcgc cggcgacgcg ggcaaggtcg ttccccagct tcgccagatc
      901 cttggcgacc gccccatcaa catcatcaag cgcaccactc cgtaacggcc cgcctgcgtg
      961 aagattcgtc cgtctcgatc ctgcccgatc cgtacgggcg aatgaaaatt cgcccgtggg
     1021 gcccttcgct tcgctcaggg gtcgaggagc tgccgtacgc cccccgtctg acgcagtcgt
     1081 gcctgtggcc ggccatgaag tgtccggttc gaggggcctc accggagcac ttatgggccg
     1141 gcccgatccg ccctacgatc cgtacgggcg aatgaaaatt cgcccgcctg ctttccttgt
     1201 acgggcggat tacatccgcc cgctgcggtc gagcagaatg acttccatcg cgcccgatgc
     1261 ttctgtaagt gtggattgac ttcctggtca acaggacaaa ttctatcctc gttcattggg
     1321 cggaacggcc ccgggatgtg cagtgatgaa cggaaagaaa attgatctgc cagtaatcgg
     1381 gatgtcttgc gcacactgcg cggccaccgt tgaaaagacc ctggtcaggg atgtgcccgg
     1441 cgtgtcctct gctgtggtga atctggcaac tgaaagtgtc gccgtgacat tcgacgaacg
     1501 tactacttca ccgcaggcga tggccgacgc cgttgccagg gcgggtttca agctggtact
     1561 gcccagcctg gacgacgtcg aaacgaacgc gcgcaggcaa aacgagcgaa ggatcaggcg
     1621 ggagcttctg gtcggaattg ttttcgcatt acccctgctc gtcctttcaa tgggttccga
     1681 cttcggcctt ctgggcgggc tgtcgcatgc cccctggctg ggatggattt acctgacgct
     1741 tgcaaccccg gtacagttca tcactggagc atccttttac atcggagcct atcgggccgt
     1801 caggaaccgc gccctggata tggacgtgct ggtcgccctg ggctcgaccg tcgcctatgt
     1861 ctattccctg gtgcttctgg tgacacccgg ccacgaacac ctgtactttg aaacgtccgc
     1921 catgattgtc gtgctgataa aggtcggcaa gatgctcgag gccagatccc gagcggtcgc
     1981 tgcacggtcg atcaagagcc ttcttgattc agcgcctgat accgcgtcgg ttatcggccc
     2041 ggacggccag gagcgcccca tcgctgtcgc cgcactttcc gttggtgatc ttgtaatggt
     2101 tcgtcccggc gaaaaggttc cggttgacgg aactgtcgtt tccggagcct cggccgtcga
     2161 tgaaagcatg gtgaccggcg agccgattcc cgtcgataag gttcccgggg acattgtatt
     2221 tggcggaaca atgaatgcgg aagggctcct taccgtccgt gcgaccggtg tcggcgcgga
     2281 ttccctgctg gcccgcatag gcgcaatggt cgccagggcc caggccagca aggcccccat
     2341 ccagcgcttc gccgatcggg ttgccgccgt tttcgtcccg gtaatcctgg taacggccgc
     2401 cgtcaccttc actttctgga tgctctggac cgacggcgat atccagcttg cagtaatgcg
     2461 ttcaatcgcc gtgctggtca tcgcgtgccc ctgtgcgatg ggacttgcca cgccgatggc
     2521 cgtcatggtc ggaatgggga ggggcgcccg cggcggaatc ctgttcaggg acgcagccac
     2581 aatggagatg cttcacaagg tcacagcaat cgccttcgac aagaccggca ccttgaccca
     2641 gggccgtccc gagatcgccg catgggtttc gacccctgga tgggaacaga ctgggcttga
     2701 acttgccgcc ggcgcggaat ccggttccgg tcacccgctt gcacaggccg tggttcggta
     2761 tgcccgcagc ctggatgtct ccgttccgcc accctccgac ttccaggcga cccctggcat
     2821 cggtgttcgc gcgacggttt ccgaacatgc cgtccaggtt ggcaacccgc gtcgtcttga
     2881 cggcatcgct atccccgact ggatggccga caggatgcac gggcttgaat ccatgggcca
     2941 ttccgtcctg gccgtgttgg ttgacggcga aacggtcggt cttttcttcc tggccgatca
     3001 gcttcgaccg accgccgtcg gcgccgtcgc cgagatgcgg gaaatgggac tcaacctttc
     3061 catgctgacc ggcgacaacg catccgctgc cgcccgggtc gccgccgctg ctggaatcgc
     3121 ggttcaccag tccggcatga tgcccgccga caaagaggcc gccatcgccg aacttcagca
     3181 aggcggccat cgggtcgcca tggtcggcga cggcatcaac gacacgcccg cactggcgcg
     3241 ggccgatatc ggaatcgccc ccggcgccgg ggccgccgcc gcaatcgaaa ccgccgacgt
     3301 gacacttgtg ggcagcgacc tgaccctggt tgcccgcgcc gtgaagctgt cccgcgcaac
     3361 catccgcaag atccgccaga accttttctg ggctttcttc ttcaacgtac tgtgcgtccc
     3421 aatcgccgcg ggcgtcttcc acggcgtcga atggatgccc gccatgctgc gcgaccttaa
     3481 ccccatgctc gccgccaccg ccatggcctt ttccgacatc atcgtcatcg gcaacagcct
     3541 ccgcctgaac cacgtccggc tgccttgatc ttcccttggc cttgtgcagg cgggcgaatt
     3601 ctcattcgcc cgtacggata gcgggcaggg aagcgatggg cgcgatggac ggcatcctgc
     3661 tcgaccgcgg cgggcggatg taatccgccc gtacaaggaa gggatgcagg caggtggaac
     3721 tggcaacttg cagtgccgcg gcgggcggat gtaatccgcc cgtacaagga agggatgcag
     3781 gcaggtagaa ctggcaactt gcagtgccgc ggcgggcgga tgtaatccgc ccgtacaaga
     3841 gcaacggcgc acaacgccca gacaaagccg gaggcgggcg aatttactgg acttcagcga
     3901 cgaaggcgtc gggggtgaag ggcttgccga aggcggtctt gacgaactcg ggccagggct
     3961 gtgtcatgcc gggctcgaag atctgggcga tgatgaagcg accgaagtcg gccttgccga
     4021 cgaagtcgag ttcgctggcg ggaccggtgt gggcggcgat ttcggacagc ttggcgcgca
     4081 gcatggtcgc gaacagttcg cccagcagat agttgtggta atagaccggc gcgattgtga
     4141 agtgcggctt ggcggcccag tcggcggccg tgcgctcggc cggacggaca agaccctgaa
     4201 gttcgacgac gcggtcgtac cagatcttgt taagttccgg gttgtccggg ttcgtgtaca
     4261 ggtccttttc aaagaagaac atgaccatcg cccagcgcgc gaaaatcaac tg
//
LOCUS       DAOPPA010000275         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22235_length_4308_cov_4.671056, whole genome shotgun sequence.
ACCESSION   DAOPPA010000275 DAOPPA010000000
VERSION     DAOPPA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22235_length_4308_cov_4.671056"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKH54_08940"
     CDS             complement(<1..242)
                     /locus_tag="PKH54_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00061.1"
                     /translation="MKIVSVEFENLNSLAGHWKIDFTDPGYVESGIFAITGPTGAGKS
                     TILDAICLGLFGRTPRLGKFGTTSGNEIMTKGKADC"
     gene            complement(239..1519)
                     /locus_tag="PKH54_08945"
     CDS             complement(239..1519)
                     /locus_tag="PKH54_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HOD00062.1"
                     /translation="MRLLHTSDWHLGARLHGQSRTAEFENLMEWMLGVIRDNSVDVLV
                     VSGDIFDSSVPGSGNQGLYYRFLASLVATCCRNVVIIAGNHDSPSLISASEELLRLLN
                     IYVVGAARENPEDEVVTLHDGAGNAMAVVAAVPYLREGDVRLSEAGEGAEAKLDKYAT
                     GIASHYDRVCTHLHKVRQEAVALARGRHVPAVVTGHLYVIGSQDSESEHKLYVGNLGG
                     VDGGMFPEWVDYVALGHLHKPQRIGDRQNFRYSGSPLQLGFDAADQNRFVYLVDFPDV
                     PGMPPDVAHIQVPVWQKLGSVHGKSWDEIERALTALKSAGESIWIDVHYEGDHDLSDL
                     AGRVHAAVDDSCVQALRIHDHQRKSAVMLAKSPDEKLEDLEPVEVFRRRLAATTVLPD
                     DQAALEAMFDEILYALRNRDGSAEPSPGDEGGAR"
     gene            complement(1620..2081)
                     /locus_tag="PKH54_08950"
     CDS             complement(1620..2081)
                     /locus_tag="PKH54_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00063.1"
                     /translation="MNLEELVSLLERFQVEEVARKPLPLMYPELKFEIVDGDIWVSRG
                     PDNVFGWFEPLDDGNWNVRVFLTAAIDCDDAIDEERREEAVGRMESEMWPAFRERGYA
                     IDYVEDEWHSGEDFFQRCVVKQVVNSAEILAEACFLMRAYISDRFRTERIG"
     gene            2276..3526
                     /locus_tag="PKH54_08955"
     CDS             2276..3526
                     /locus_tag="PKH54_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00064.1"
                     /translation="MTTTRKTQAPQEDAVEIIPHKWEFKARFRRGSFGWRSQPAIQRV
                     KEAVAEITKVARRNPVLGAEGAVIFLERVSPALEQVDSSSGAIGTAVCNAIRVLVPII
                     AGAPADARTRTIWLERLFQASVGDDVAYIDILTDYWGELCASKGVASQWADDLIGTTR
                     LALGPDKRLHGYFHGTSACLSALLTAERYDELIELLQHERFWAHKQWAVRALAARGRN
                     DEAIEMAEACRGPWANNFQIDRICEEILLTCGRIDEAYARYGLSANRCGTNVATFMAV
                     TRKYPTKAPAEILADLVKSTPGEEGKWFAAAKDAGLFREALFLAERSPCDPKTLTRAA
                     RDHIDRQPAFALGAGLLALYWLFQNHGYKITSPDILAAYNNTIDAAGRTGQIDATVKK
                     INRMATSLMGGNSFAAHILEDKLH"
     gene            3737..4003
                     /locus_tag="PKH54_08960"
     CDS             3737..4003
                     /locus_tag="PKH54_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00065.1"
                     /translation="MTLIPSIVGMVGAAMILLAYLLLQMGRMTTEGLWYTVVNLVGSL
                     MILFSLFFDFNLPALFIEVVWISVSVVALVRLFMKRRRARGPEA"
     gene            complement(4005..>4308)
                     /locus_tag="PKH54_08965"
     CDS             complement(4005..>4308)
                     /locus_tag="PKH54_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00066.1"
                     /translation="RRHFPDIAYYRGPGGREVDFVVRRSDGGLDLVQVCENMADSATR
                     HREVTGLAEAMKALNVSTGTIVTLDESSTVDADHGLITVVPAWKYMLGLAEDGFRP"
BASE COUNT          976 a         1307 c         1166 g          859 t
ORIGIN      
        1 atgcaatctg cctttccctt cgtcattatc tcgttgccgc tggtcgttcc gaactttcca
       61 aggcgcggag tccttccgaa caggcccagg cagattgcgt ccaggatcgt tgatttgccc
      121 gcaccggtcg ggccggtgat ggcaaatatg ccggactcga catagccggg atccgtgaaa
      181 tcaatcttcc agtggcccgc cagcgaattc aggttttcga actcgaccga gacaattttc
      241 atcgcgcacc cccttcgtcg ccgggcgaag gttcggcgga tccgtcgcga ttccttaatg
      301 cgtacaggat ttcatcgaac atcgcttcaa gcgccgcctg atcatccgga aggacggtcg
      361 tggcggccag tctgcgcctg aagacttcga ccggttccag atcctccagt ttttcgtccg
      421 gggacttcgc cagcatcacg gccgactttc gctggtggtc atgaattcgc agagcctgaa
      481 cgcacgagtc atcgacggcc gcgtgcacgc gaccagccag gtcggacaag tcatggtcgc
      541 cctcgtaatg cacatcgatc cagattgatt cgccggctga cttcaaggcc gtcagcgccc
      601 tttcaatttc gtcccacgac ttcccgtgaa cagacccaag tttctgccac acaggaacct
      661 gaatgtgggc gacatccggc ggcatccctg gaacatcagg aaaatcgacc agatacacga
      721 acctgttctg gtcggctgca tcgaaaccaa gctgaagcgg cgacccggag tacctgaaat
      781 tctggcgatc ccctatgcgc tggggtttgt gcagatggcc cagcgccaca taatcgaccc
      841 attccgggaa catcccgcca tcaacgcccc ccaggttccc gacgtacagc ttgtgttcac
      901 tttccgagtc ctggcttcca atcacataaa gatggcccgt gacgaccgcc ggcacatgac
      961 gaccacgcgc cagggcgacg gcttcctgac ggaccttgtg aaggtgcgtg cacacccggt
     1021 cgtaatggct ggcaataccg gttgcgtact tgtcgagctt cgcttcagcg ccctcaccgg
     1081 cctcggaaag cctgacatcg ccttcacgca gataaggcac tgccgccacg acggccatcg
     1141 catttccagc cccatcatgc agggtgacta cttcatcttc ggggttttcg cgggccgccc
     1201 ccacaacata gatattcaac agtcgcagca gttcctcgga cgcggaaatc agcgacggcg
     1261 aatcgtggtt accggcgatt atcacgacgt tcctgcagca cgttgccacc agggaagcca
     1321 gaaaacggta ataaagtccc tgatttcctg accccggcac cgaactgtca aaaatgtcgc
     1381 cggacacgac cagcacatcg acgctgttat cccggatgac accgagcatc cattccatca
     1441 ggttttcaaa ttcggcggtg cgcgactgcc cgtgaagccg cgcgccaagg tgccagtcgg
     1501 atgtgtgcag caacctcatt tcatccctct gctgtcgaga aagcgaagcg tacatcaccc
     1561 agcggcaaga tttctgccgc aaaagatatg gcgctcggac gcgaccgacc agttccgaat
     1621 caaccaatcc gttccgtgcg gaatctgtca gatatgtacg cgcgcatcag gaaacacgct
     1681 tccgccagga tctccgctga attcacaacc tgcttgacca cacatcgctg gaaaaagtct
     1741 tcaccggaat gccattcatc ctcgacataa tcgatcgcgt atccccgttc cctgaaggcc
     1801 ggccacatct cggactccat acgtccaaca gcctcctcgc ggcgctcctc gtcaatggca
     1861 tcgtcgcaat cgattgccgc agtaagaaac acacgaacat tccaatttcc gtcatccagc
     1921 ggctcgaacc agccgaacac attgtcaggg ccacgggaca cccagatatc accgtcaaca
     1981 atttcgaatt tcagttcagg atacatgagt ggaagcggtt ttctggcaac ttcctccact
     2041 tgaaagcgtt ccaataacga aaccaattct tcaaggttca tcttgtgagg ctccataatt
     2101 caagtacgga tgataatcta acacatcgac ggaccttaca caggccaggc cgaatcgggc
     2161 tggcatcgtg tgcccaattg gagccgttcc gaacatgcaa gcacggccac gatgaacgcc
     2221 aggaatggag caaagttaac ccaaacagcc ccttgatgcg gccgtgggga tgaagatgac
     2281 cacaacgcgc aagactcaag ctccccaaga ggacgcagtt gaaatcatcc cgcacaagtg
     2341 ggaattcaag gcccgttttc gacgcgggtc attcggatgg cggtcacaac cagccatcca
     2401 gcgcgtgaag gaagccgttg ccgagatcac gaaggtcgcg cgaaggaatc cggtgctggg
     2461 tgccgagggt gcggttatct tcctggaacg cgtttcgccg gcactcgagc aggtcgatag
     2521 ttcgtcgggt gcaattggca cggccgtctg caacgcgatc agggtcctgg ttccaattat
     2581 tgccggggcc ccggcggacg ccagaacccg caccatatgg ctggaacgac tttttcaggc
     2641 aagcgtcggc gacgatgttg cgtacatcga cattctgacc gactactggg gcgaattgtg
     2701 cgcgtccaaa ggggtggcgt cccagtgggc cgacgacctt atcgggacga cgcgactggc
     2761 gcttggccct gacaagcggc tgcatggata cttccatgga acatcggcct gcttgagcgc
     2821 ccttctgact gccgaacgat acgacgagct tattgaattg ctccaacatg aaagattctg
     2881 ggcgcacaag caatgggcgg tcagggcact ggcggcccgg ggcaggaatg acgaggccat
     2941 cgaaatggcc gaggcttgcc gcgggccctg ggccaacaat tttcagattg acaggatctg
     3001 cgaggaaatc ctgctgacat gcggcaggat cgacgaagcc tatgccagat acgggttatc
     3061 ggccaaccgc tgcggcacca atgtggcgac attcatggcg gtcacacgga aatacccgac
     3121 caaggccccc gccgaaatcc ttgcagacct tgtcaaatca acgcccggcg aggaaggaaa
     3181 atggttcgcc gccgcaaagg acgcgggact gttccgcgag gcgcttttcc tggccgaacg
     3241 ttcgccctgc gatccgaaaa ccctgacccg cgcggcccgg gatcatatcg atcgccagcc
     3301 tgcattcgcg cttggggctg gtcttctggc gctgtattgg ttgttccaga atcacggata
     3361 caaaattacc agccccgata tactggccgc atacaacaac acgatcgacg ccgccggacg
     3421 aaccggccag attgatgcga ccgtgaaaaa gatcaaccgg atggcaactt cgcttatggg
     3481 cggaaacagt ttcgcggccc acattcttga agacaaactg cactgatggc aacgggagtc
     3541 gccttgtgtc taaccacctg ataccgtgtc cagatttttt cgttgacaaa actatccaag
     3601 ttgggaacat tccgaagtcg gcgcagcact aaaaacccgc aacttccagg cgttacaact
     3661 acaaccaaag acaagaagac cgaaaagctc aacgacagtg gcataatcgg gccgggattt
     3721 gcggggtggg gcggcaatga ccctgatacc aagcatcgtc gggatggtcg gcgcggcaat
     3781 gatattgctg gcgtacctgc tgctgcagat gggaaggatg acgaccgagg ggctgtggta
     3841 cacggtcgtg aacctggtcg ggtcgctgat gatcctgttt tccctgtttt tcgacttcaa
     3901 cctgcccgcg ctgttcatcg aggtcgtgtg gatttcggtc agcgtcgtgg cgcttgtacg
     3961 cctgttcatg aagcgccggc gcgcaagggg cccggaagct tgattcaggg acggaagcca
     4021 tcttcggcaa ggcccagcat gtatttccag gcaggaacaa cggtaatcag cccgtgatcg
     4081 gcatccactg ttgaagattc gtccagagtc acgatcgttc ccgtcgaaac attcagtgcc
     4141 ttcatcgcct ctgccagccc tgtgacttca cggtgacgtg tcgccgaatc ggccatgttt
     4201 tcacagacct gcaccagatc aagcccgcca tcggatcgcc gcacgacaaa atcgacctcg
     4261 cgaccgcctg gccccctgta ataggcgatg tccggaaaat gccgcctg
//
LOCUS       DAOPPA010000276         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22344_length_4290_cov_3.910508, whole genome shotgun sequence.
ACCESSION   DAOPPA010000276 DAOPPA010000000
VERSION     DAOPPA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22344_length_4290_cov_3.910508"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /gene="thrS"
                     /locus_tag="PKH54_08970"
     CDS             <1..1498
                     /gene="thrS"
                     /locus_tag="PKH54_08970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOD00067.1"
                     /translation="KAEALELFATNKFKVEMINDLQEGTISIYKQGEGETAFMDLCRG
                     PHVPSTGYLKAFKLTKFAGAYWRADQSREQLQRLYGISFPNKEMLDEHLKKIEEALAR
                     DHRKIGQEMELFTFHDEGTGFPFWHANGMVLKNIVVDYWRNVHKRYGYNEIQTPQMLN
                     KVLWEMSGHYDHYRNNMYFTSIDDIPYAVKPMNCPGGLLVYKSRMHSYREFPLRNAEL
                     GLVHRHEMSGVLHGLFRVRAFTQDDAHVFCTPDQVKSEVATIIDMVFEIYKTFGFNDV
                     HIELSTRPDNSIGSDEMWANAEGGLRDALELRGINYKLNPGDGAFYGPKIDFHVKDSM
                     GRSWQCGTIQVDFSMPERFHATYEAEDGTRKTPVMLHRAILGSLERFIGILIENYAGK
                     FPMWLNPVQVKVLPVSDQFNDYAKEVVAKFCAAGLRAEADLRSERLGRKIRDAQLSRV
                     NYQVVVGAAEAENGTVSVRTRKNENLDALPVDEFTARMVNEATSRAAE"
     gene            1565..2458
                     /locus_tag="PKH54_08975"
     CDS             1565..2458
                     /locus_tag="PKH54_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD00068.1"
                     /translation="MVRTRRSIEEEARVMLVSGPGTDRFLQDEGLSDQIEGDAIDQML
                     AWGCETARRALVVGLEDRGRIALELARRGVFVTVVEPDESLHEPVRAAAEAEHFMIRL
                     NFYASDYMKREFASSGFDLAIFYSALSRYNEPLVVLKKAARELRAGGKVFARIRVRPA
                     MGKIRGYVDPIFEKVSEKAVRVPGFVTVRDRIINAIERLPLVSRFVGLPDAGDMLEDL
                     AGVFKIERVEKRHLLAPVVGWAGVQDVVPDAARGLVRRAVPAVVKADRAMLRAAGDRL
                     VASHLYVYGAKELGLGHTFRV"
     gene            2486..3859
                     /locus_tag="PKH54_08980"
     CDS             2486..3859
                     /locus_tag="PKH54_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD00069.1"
                     /translation="MIESGSRTTVTSISRSVTLSGICLRIAGQPDFLIDSDTVTVGAA
                     RECDLVIDDPGVSRTHFQILRRPEGWLLRDLKSTNGTMVERARVVEAWLSTGMTITIG
                     RTSIEFMAVSDMAPVSASASEGFGDAVGRSQAMREIFSLLERVSQTDATVLIEGETGS
                     GKEVIARGIHDASRRAQGPFVVFDCGATPRDLVESILFGHERGAFTGAVTTREGLFEQ
                     ADGGTLFLDEVGELPPEVQPKLLRILESREVRRVGGSKARKVSVRLVAATNRRLESEV
                     AAGRFRADLFFRLAVIRVTLPALKDRIQDIPLLVSAFTSRRRAVHGSCPGVSDRALSI
                     LSQWNWPGNVRELFNALERAASLCPGTVVDVADLPPYILDAVLPGRKPGDDGPTVPLD
                     RIVRPWASAREDCINRFERDYLADLMEKCGGNLTVAARCADLDRKHLRNLLKKHGLYR
                     GPDPIAE"
     gene            complement(3887..>4290)
                     /locus_tag="PKH54_08985"
     CDS             complement(3887..>4290)
                     /locus_tag="PKH54_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00070.1"
                     /translation="RPSSMSACFTHDRRLFSPCQECPLGNDRFYLWKTEQSITSAQDA
                     SAICSTSEFMYIDFEERYEVALDTGTYLACSRNMDYQNAMNCVDVLIAEGMITTLNTE
                     HMGWTPALYLSEPGEAPVLIDNPILATCIRQ"
BASE COUNT          860 a         1227 c         1353 g          850 t
ORIGIN      
        1 gaaggccgaa gcgctggagc tgtttgccac caacaagttc aaggtcgaaa tgattaatga
       61 tcttcaggaa ggcaccatca gcatctacaa gcagggcgag ggtgaaaccg ctttcatgga
      121 tttgtgccgc ggcccccacg ttccttcaac cggctacctg aaggcattca agctgaccaa
      181 gttcgcgggt gcatactggc gcgccgacca gagccgcgag cagcttcagc gtctttacgg
      241 aatcagcttc ccgaacaagg aaatgctgga tgaacacctg aaaaagattg aagaagccct
      301 tgcgcgtgat caccggaaaa ttggccagga aatggaactt ttcaccttcc acgacgaagg
      361 aaccggcttc ccgttctggc acgcgaacgg catggtcctg aagaacatcg tggtcgatta
      421 ctggcgcaac gttcacaaga ggtatggtta caacgaaatt cagaccccgc agatgctgaa
      481 caaggtgctg tgggagatgt cggggcatta cgatcactac cggaacaaca tgtacttcac
      541 ctcgattgac gacattccgt atgccgtcaa gccgatgaac tgtcccggcg ggctgctggt
      601 ctataagtcg cgcatgcaca gttaccgaga atttccgctg cgcaacgcgg aactggggct
      661 ggtgcaccgc cacgagatgt cgggcgtcct tcacggactg ttccgcgtcc gcgccttcac
      721 ccaggacgac gctcacgtgt tctgcacccc ggatcaggtc aagagcgagg tcgcgaccat
      781 aatcgacatg gtgttcgaga tttacaagac gttcggattc aacgacgtgc acatcgaact
      841 ttcgaccagg ccggacaatt cgatcggttc cgacgagatg tgggccaacg ccgaaggcgg
      901 tctgcgggat gctcttgaac tgcgcggaat caattacaag ttgaacccgg gcgacggcgc
      961 gttctatggc ccgaagatcg acttccacgt caaggacagc atgggccgtt cgtggcagtg
     1021 cggcaccatc caggtcgatt tctcgatgcc cgaaaggttc cacgcgactt acgaggccga
     1081 agacggcacc cgcaagaccc cggtcatgct tcaccgcgcc atccttggct cgctggaacg
     1141 cttcatcggc atcctgatcg agaattacgc cggcaagttc ccgatgtggc tgaacccggt
     1201 tcaggtgaag gttctgccgg tttccgacca gttcaacgat tacgcgaagg aagtggtcgc
     1261 gaagttttgt gccgccggtc tgcgcgccga ggccgatctg cggtccgaaa ggcttggcag
     1321 gaagattcgc gacgcccagt tgtcccgcgt caactaccag gtggtcgtgg gcgcggccga
     1381 ggccgaaaac ggcaccgtca gcgtccgtac caggaagaac gaaaatcttg acgcgcttcc
     1441 agtggacgaa ttcactgcaa ggatggttaa cgaagccacc agcagggccg ccgagtgacg
     1501 gccgcgacgg ttcgcccgat ttcgggcccg atccgggcca tgcatatttg aagggattca
     1561 ccagatggta agaaccagac gttcaattga agaagaggcg agggtgatgc tggtttccgg
     1621 tcccggaacg gacaggttcc tgcaggacga gggcctttcg gaccagatcg agggcgacgc
     1681 gatcgaccag atgctggcct ggggttgcga gacggccagg cgtgcgctgg tggtcggcct
     1741 ggaagatcgc ggccggatcg cgctggaact tgcgcgacgc ggtgtcttcg tgaccgttgt
     1801 cgagcctgac gagtcgcttc acgagccggt ccgggccgca gccgaagccg agcacttcat
     1861 gattcgcctg aacttctatg cgtccgacta catgaagcgt gaatttgcct caagcggttt
     1921 cgacctggca attttttatt cggctctttc ccgttacaac gagcccctgg tcgtcttgaa
     1981 aaaggcggcc cgcgagttgc gtgccggcgg caaggtcttc gcgcgcatcc gggtgcgccc
     2041 ggccatggga aagattcgcg gctatgtcga tccgattttc gagaaggtaa gcgagaaggc
     2101 cgtgcgcgtt cccggcttcg taaccgtcag ggacaggatc atcaacgcga tcgagcgcct
     2161 gcccctggtg tccaggtttg tcgggctgcc cgatgccggg gacatgctgg aagaccttgc
     2221 cggcgtgttc aagattgaac gagttgaaaa gcggcacctg ctcgcgccgg tcgtcggctg
     2281 ggccggcgtc caggatgtcg tgccggatgc ggccaggggc ctggttcgca gggcggtgcc
     2341 ggcggtcgtc aaggcggatc gggccatgct tcgcgcggcc ggggatcggc ttgtggcaag
     2401 ccacctttac gtgtatggcg ccaaggaact tggcctggga cacacgttcc gggtctgatg
     2461 cccgtcttgt gccagggggc ctgcaatgat cgaatccggc agccggacaa cggttaccag
     2521 catcagcagg tcggtgacgc tttccggtat ctgccttcgc atcgcgggcc agcccgactt
     2581 cctgattgat tcggacacgg tgacggtcgg tgcggccagg gaatgcgacc ttgtcatcga
     2641 tgatcccggt gtcagccgga ctcactttca gatcctccgc cgaccggaag gctggctgct
     2701 tcgcgacctg aaatccacga acggtacgat ggttgaacgc gctcgggtgg tcgaggcctg
     2761 gctttcgacc ggcatgacca taaccatcgg ccggacatcc atcgaattca tggccgtttc
     2821 cgacatggcc ccggtcagtg cctcggcaag cgagggcttc ggtgatgcgg tcgggcgcag
     2881 ccaggcaatg cgggaaattt tctcgcttct tgaacgcgtg tcccagaccg acgcgaccgt
     2941 gctgatcgag ggcgaaaccg gttccggaaa ggaagtcatc gcccgcggca ttcacgatgc
     3001 cagccgtcgt gcccaggggc cgttcgtcgt tttcgactgc ggggccaccc ctcgcgacct
     3061 tgtggaaagc atcctgttcg gccacgaacg cggcgccttc accggggcgg tgacgacgcg
     3121 ggaaggcctt ttcgagcagg cggacggcgg aaccctgttc ctggacgaag ttggcgagct
     3181 tccacccgaa gtccagccga agctgctgcg catcctggaa agccgcgagg tcaggcgcgt
     3241 gggcgggtcg aaggcccgca aggtgtcggt caggctggtc gcggcaacca atcgcaggct
     3301 ggaatcggaa gtcgctgccg gcaggttccg ggcggacctg ttcttcaggc tggccgtcat
     3361 tcgcgtgacg ctgcccgcgc tgaaggatcg gattcaggac attcccctgc tggtttcggc
     3421 cttcacgtcg cggcgaaggg ccgtccatgg aagctgcccc ggcgtgtccg atcgcgcgct
     3481 ttcgatcctg tcgcaatgga actggccggg aaacgtgcgg gaactgttca acgcactcga
     3541 gcgcgcggct tcgctgtgtc ccggaaccgt ggttgacgtg gccgacctgc cgccttacat
     3601 cctggacgcg gtgcttcccg ggcggaaacc gggcgacgac gggccgactg tcccgctgga
     3661 tcgcatcgtg cgtccctggg caagcgcccg cgaggactgc ataaaccggt ttgaacgcga
     3721 ttatctggcg gatcttatgg aaaaatgcgg tggcaacctg acggtcgcgg cacgctgcgc
     3781 ggacctggac cggaaacatc tgcgtaatct tttgaaaaag cacggactgt accgggggcc
     3841 tgacccgatt gctgaataac aaccaccgtt gtaatcgatt attttgtcat tgccttatac
     3901 aggtggccag aatcgggttg tcaatcagga cgggggcttc gccaggttct gacaaataca
     3961 gggcaggggt ccagcccatg tgttcggtgt tgagcgtggt tatcatgcct tcggctatca
     4021 gaacatcgac gcagttcatc gcattctgat aatccatatt acgcgagcag gccaggtagg
     4081 taccggtatc aagtgcgact tcgtatcgtt cttcaaaatc gatatacatg aactcagacg
     4141 ttgaacagat agctgatgcg tcctgagctg acgtgatgga ctgttctgtt ttccacaaat
     4201 aaaagcggtc attgcccaga gggcattcct gacagggtga aaacagtctc cggtcatggg
     4261 tgaagcaggc ggacatcgac gaaggtcggg
//
LOCUS       DAOPPA010000277         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22431_length_4275_cov_4.145735, whole genome shotgun sequence.
ACCESSION   DAOPPA010000277 DAOPPA010000000
VERSION     DAOPPA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22431_length_4275_cov_4.145735"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="metG"
                     /locus_tag="PKH54_08990"
     CDS             <1..1462
                     /gene="metG"
                     /locus_tag="PKH54_08990"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOD00071.1"
                     /translation="TYSLCTLEFEAHRPLYDWFIQNVGAFPSRQIEFARLNLTYTVMS
                     KRKLLELVQKKLVDGWDDPRMPTICGLRRRGYTPESIKAFAERIGVAKKDSMVDLALL
                     EHTVREDLNARVPRYMAVIDPLKVVIENLPEGEVIEFDAPLSPEDPNLGTRKIALTRE
                     VFIEREDFMEVPSKKWFRLSPGAEVRLRYACLLKCHGVVKDESGRVLELRCTWDPESK
                     GGVSPDNRKVKGTLHWVSAAQAIGAEVRLYDKLFTIENLGDMPEGASYIDYLNPESLV
                     VVNGARLEPAMAALKPGDRVQFERVGYFCVDTKDSKPGALVFNRTVGLKDSWQKVAAK
                     EEGEAPAAKAEPAKAEAAKAPAPTPTIPANLAVPEAHPNFKELAPEITIDEFLKVDLR
                     VAIIRHAEAVPEADKLLRLLVDVGEGRLRQIFAGIKKAYDPATIIGKRIIIVANLKPR
                     QMKFGLSEGMLLAAGEVPDLGLATFDRDVTPGDRIG"
     gene            complement(1489..2712)
                     /locus_tag="PKH54_08995"
     CDS             complement(1489..2712)
                     /locus_tag="PKH54_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00072.1"
                     /translation="MYCIRTIENNLRKVSEQFRVVLITGPRQVGKTTLLRHLGGPDRR
                     FVTLDDPAVLQIAREDPGLFFQRFPPPITIDEIQYAPGLLTYIKMIVDDDGTPGRFWL
                     TGSQQFPLMKGVSESLAGRIAIINILGLSYAEADGRGSMSLPFIPGGPGFEERIRDAA
                     RPDLQDVYARIFRGSMPELVLNPDLDPAVWFGSYVQTYIQRDVRDLARVGDEMAFLRF
                     LRATAARTGTLLNLADLARDADIAPNTAKNWMSILVTSGIVHLLEPWFRNQTKRIVKA
                     PKIYFLDTGLCAYLTGWPSASTLETGAMTGQILETWVVAELLKTFWHNAKRAPLYFMR
                     DRDGREIDILIQNGDTLHPFEVKKSAMPGRDDIRHFQMLDSTGMNVGEGGVFCLAGEF
                     MPLNERNRSIPITAI"
     gene            complement(2804..3277)
                     /gene="rimI"
                     /locus_tag="PKH54_09000"
     CDS             complement(2804..3277)
                     /gene="rimI"
                     /locus_tag="PKH54_09000"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOD00073.1"
                     /translation="MAAVRAAQTPGRITLSPMTSADIPAIVDLEAASFPEDPWPLEAF
                     VHELSNEFSQPMVAWDMDGNLIGYMINWFIGPEVHIANIAVDPECRGMGYGREMMKQA
                     IRMSREAGAEEMVLEVRIGNVPAINLYVSLGFQKVYIRKFYYRNGEDAIVMALAL"
     gene            complement(3288..>4275)
                     /locus_tag="PKH54_09005"
     CDS             complement(3288..>4275)
                     /locus_tag="PKH54_09005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOD00074.1"
                     /translation="KGRVIVSLFASNVMRVRALVEIGEKYGRRTAMIGRSMEIYGEAA
                     QKAFMMRPPAGLVDERSVERIPDGELLVLCTGSQAEPRSVLNRASLMDHPDLKIHPGD
                     TVVFSSRIIPGNEKAIFKMINNLTRLGARVIHERTANVHGSGHAQRDELEEMIRAVRP
                     EVMVPMHGDYRFLVAHKDLAEACGVEDVRILENGHQFELSKHDALITDHVSLNFHYVD
                     GPLVGDADELRLAERRRIAWNGVIAACLKVSRGRKEWEVALKIHSVGVPYFDEETVGM
                     ARRYATEQLLELPLDSSRQVIEDTLVSSLRSYVRRITEHKATVLPFIEFADD"
BASE COUNT          816 a         1318 c         1315 g          826 t
ORIGIN      
        1 cacgtattcg ctgtgcaccc tggaattcga ggcgcaccgc ccgctttacg actggttcat
       61 ccagaacgtc ggcgcgttcc cgtcgcgcca gatcgagttc gcgcgcctga acctgaccta
      121 caccgtcatg tccaagcgca agctgcttga actggtgcag aagaagctgg tggacggctg
      181 ggacgacccg cgcatgccga ccatctgcgg gctgcgccgt cgcggctata cccccgaatc
      241 gattaaggct ttcgccgaac gcatcggcgt cgccaagaag gacagcatgg tcgatctggc
      301 cctgctggaa cacacggtcc gcgaagacct gaacgcccgc gtgccgcgct atatggccgt
      361 catcgacccg ctgaaggtcg tcatcgagaa cctgccggaa ggcgaagtca tcgagttcga
      421 cgcgccgctg tcgcccgagg atccgaacct gggcacccgc aagatcgccc tgacccgcga
      481 ggtcttcatc gagcgcgagg acttcatgga agtcccgtcc aagaagtggt tcaggctgag
      541 ccccggcgcc gaagtccgcc tgcggtacgc gtgcctgttg aagtgccacg gcgtcgtcaa
      601 ggacgaatcc ggtcgggtgc tggaactgcg ctgcacctgg gatcccgaat cgaaaggcgg
      661 cgtcagcccc gacaaccgca aggtcaaggg caccctgcac tgggtttccg cggcccaggc
      721 catcggggcc gaagtccggc tgtacgacaa gctgttcacc atcgagaacc tgggcgacat
      781 gccggaaggc gcgtcctata tcgattacct gaacccggaa tcgctggtcg tggtcaacgg
      841 ggccaggctg gaaccggcca tggcggccct gaagcccggc gaccgcgtgc agtttgaacg
      901 cgtcggttac ttctgcgtgg atacaaagga ttcaaagccc ggggcgctgg tgttcaaccg
      961 cacggtcggc ctgaaggatt cctggcagaa ggtcgcggca aaggaagagg gcgaggcccc
     1021 ggccgcgaag gccgaaccgg cgaaggccga ggctgcgaag gccccggcgc cgacgccgac
     1081 aatcccggca aacctggccg ttcccgaagc gcacccgaac ttcaaggaac tggccccgga
     1141 aatcaccatc gacgaattcc tgaaggtcga tttgcgcgtc gcaatcatcc gtcatgcgga
     1201 agcggtgccg gaagccgaca agctgctgcg cctgctggtt gacgtgggcg agggccgcct
     1261 gcgccagatc ttcgcgggca tcaagaaggc gtatgacccg gcgaccatca tcggcaagcg
     1321 catcatcatc gtcgccaacc tgaagccgcg tcagatgaag ttcggcctgt ccgaaggcat
     1381 gctgctggcc gccggcgaag tcccggacct gggcctggcc acctttgacc gcgacgtcac
     1441 ccccggcgac cgtatcggct gaaaactggt ggtcggcccg gtcggccttc aaattgccgt
     1501 gattgggatt gatctgttgc gctcgttcag tggcatgaat tcaccggcca ggcagaagac
     1561 tccaccttca cccacgttca tcccggtcga atcgagcatc tggaagtgac ggatgtcgtc
     1621 gcgccccggc atggccgact tcttcacttc aaatggatga agcgtgtcgc cgttctgaat
     1681 caggatgtcg atttcccgtc cgtcccggtc gcgcatgaag taaagcggag cgcgtttggc
     1741 gttgtgccag aatgttttca gcagttcggc gacaacccag gtttccagaa tctgtcccgt
     1801 cattgcccct gtctcaagag tgctcgcgga cggccagccg gtaaggtagg cgcacaggcc
     1861 cgtatccagg aagtatattt ttggcgcctt gacgatccgt ttggtctggt tccgaaacca
     1921 gggctccaga aggtggacga taccggatgt caccagaatg gacatccagt tttttgcggt
     1981 gtttggggca atgtccgcat ccctggccag gtcggccagg ttcagcaggg tgccggtccg
     2041 ggccgccgtc gcccgcagga agcgcaagaa cgccatttcg tccccgaccc tggccagatc
     2101 ccgcacgtca cgctggatgt aggtctgaac atatgacccg aaccagactg cgggatccag
     2161 atcgggattc aacaccagtt caggcatcga gcccctgaat atacgggcgt acacatcctg
     2221 cagatccggt ctggcggcgt cccggattct ttcttcgaaa ccgggcccgc ccggaatgaa
     2281 aggcagcgac atcgagccgc gtccatcggc ttccgcatat gaaagtccca ggatattgat
     2341 aatggctatc cttccggcca gtgactcaga aacgcctttc atcaacggga actgctggga
     2401 tccggtcagc cagaatcgcc ccggagtgcc gtcgtcatct acaatcatct tgatgtaagt
     2461 cagtaatccg ggtgcgtact ggatttcgtc aatcgttatc ggtggcggga aacgctggaa
     2521 gaacaggccg gggtcttccc tggcgatctg cagaacggcc gggtcgtcga gggtgacgaa
     2581 tcgccgatcg ggcccgccaa gatgccgcag cagcgtggtc ttgcccacct gacgcggtcc
     2641 ggttatcagg acgacgcgga actgctcgga tacctttctt aaattgttct caatagttct
     2701 gatgcaatac atagcaaccc cggctaaagt gcaaccaaat tgcattttag ccgaatatac
     2761 catttaatcc aagaaattta tgatctttcg tttccaccgg cccctaaagc gccagggcca
     2821 tcacgatggc gtcttcgccg ttgcggtaat agaacttgcg gatatagacc ttctggaatc
     2881 ccagggacac gtacaggttg atggccggga cgttgccgat gcggacttca agcaccattt
     2941 cctcggcgcc cgcctcgcgc gacatgcgga tggcctgctt catcatctcg cggccgtaac
     3001 ccatcccgcg gcattcggga tcgaccgcga tgttggcaat gtggacttcc gggccgatga
     3061 accagttgat catgtagccg atcaggttgc cgtccatgtc ccacgcgacc atgggctggg
     3121 aaaattcgtt ggaaagttcg tgaacgaacg cttcaagcgg ccaggggtct tcggggaagc
     3181 tggcggcttc caggtccact atggcgggga tgtcggccga agtcatcggc gacagcgtga
     3241 tgcgtcccgg tgtctgggca gccctgaccg cggccatcgg tcccccgtca gtcgtccgcg
     3301 aattcgatga aaggcaacac ggttgccttg tgctcggtaa tccggcgcac ataactgcgc
     3361 agcgacgaca caagcgtatc ctcgatcacc tggcggctgc tgtccagcgg cagttccagc
     3421 aactgctcgg ttgcgtatct gcgcgccatc ccgacagttt cctcgtcgaa gtacgggacg
     3481 cccaccgagt ggatcttcag ggcgacttcc cattccttgc ggccacgcga aaccttcagg
     3541 cacgccgcga tgacgccgtt ccacgcgatt cggcggcgtt cggccaggcg cagttcgtcc
     3601 gcgtcgccga ccagcgggcc atcgacataa tggaagttca ggctgacgtg gtcggttatc
     3661 agcgcgtcat gcttggaaag ctcgaactgg tgcccgtttt ccaggatgcg cacgtcttcg
     3721 acgccgcacg cctcggccag gtccttgtgg gcgaccagga atcggtagtc gccgtgcatc
     3781 gggaccatca cttccgggcg gaccgcgcgg atcatttctt ccagttcgtc gcgctgggcg
     3841 tggccggagc cgtggacgtt ggcggtgcgc tcgtggatga ccctggcgcc caggcgcgtc
     3901 aggttgttga tcatcttgaa gatggccttt tcgttgccgg ggatgattcg gctggaaaac
     3961 accaccgtgt cgccgggatg aattttcagg tcggggtggt ccatcaggga cgcgcggttc
     4021 aggaccgacc tgggttccgc ctggctgccg gtgcaaagga ccagcagttc gccgtccggg
     4081 atccgctcga cgctgcgttc atcgaccagg ccagcgggcg ggcgcatcat gaacgccttc
     4141 tgggcggcct cgccgtaaat ttccatgctg cggccgatca tggcggtgcg gcggccgtac
     4201 ttttcgccga tttcgaccag ggcgcgcacg cgcatgacgt tggacgcgaa cagcgacacg
     4261 atgacgcggc ccttt
//
LOCUS       DAOPPA010000278         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22510_length_4264_cov_3.836541, whole genome shotgun sequence.
ACCESSION   DAOPPA010000278 DAOPPA010000000
VERSION     DAOPPA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22510_length_4264_cov_3.836541"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKH54_09010"
     CDS             <1..273
                     /locus_tag="PKH54_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00075.1"
                     /translation="FFQHVEQELLVAGFFPNADMRDHMMEVIRGWLTRTKPTEREIRM
                     LHGVLATLSGRRLGGKPAHVPGTKGGTGAKARKRRSQLPADAPEDA"
     gene            complement(282..629)
                     /locus_tag="PKH54_09015"
     CDS             complement(282..629)
                     /locus_tag="PKH54_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00076.1"
                     /translation="MSSAFMDPELAFSGFSYVLKDGSEGSILIDQVYDYNRDPDYIRN
                     MLLYQLTIEARRHLKASRMGRREIIRLMGTSPAQLYRLLDQTNYTKSVDQMLRLLGVL
                     HCQVRLEVLPESA"
     gene            complement(731..934)
                     /locus_tag="PKH54_09020"
     CDS             complement(731..934)
                     /locus_tag="PKH54_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00077.1"
                     /translation="MPKIRRGGFVFMAWKGDHSPNHVHVFDGRRLLMKWDLENNCLMW
                     GILNLKALALIRRLQKEGLLWRA"
     gene            1098..2147
                     /locus_tag="PKH54_09025"
     CDS             1098..2147
                     /locus_tag="PKH54_09025"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOD00078.1"
                     /translation="MMKVVDTPEKMVVSGQAVYGRFATPFRNVNLDDQKIDVFGLPVP
                     KPLRTARLKEWAHVAIVGPEVTIGCAVVDAKYLSNAWFWAADRATGKFIEHAAQCGGG
                     AIKTSRQLYNGQTDFKGGGFKVEIDDQLDKNQHVYHIEIKAGKDLPAIRADFVLHADW
                     RNNNPLVVVLPAAPGRPLYTHKSVTPVSGMIAVGNRVYQLESDKYVAMLDIQKTYYPY
                     RIFWEWATFGGYDAKGRLIGLNLCHNLIKDDETYNENCLWVEGRLHNLAPARFEYDSS
                     DYLKPWHLKTVDGSVDLEFRPEGKRDGMTDAKVLLSDYHQPFGSFHGFVTTEDGERIE
                     IDGIRGVTEDHKAKF"
     gene            2242..2715
                     /locus_tag="PKH54_09030"
     CDS             2242..2715
                     /locus_tag="PKH54_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOD00079.1"
                     /translation="MFGLDMWLVWFLAGCVIALAEMMIPGFIIVFFGLGCWGAAVVAA
                     IAPEAWAWQIAAFLVVSVSTLVGLRRLAVNLLRGKTGQPRGVASSGIEIGTVILVDVE
                     MLPGRVGRVRYRGTNWDAKSTVAITAGSQAVIVGVDEINRSCLVIEPVSGKPESN"
     gene            2732..3652
                     /locus_tag="PKH54_09035"
     CDS             2732..3652
                     /locus_tag="PKH54_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOD00080.1"
                     /translation="MASASIVALVGLAVVVLVLLVKGARIVPQKTAVIIERLGKYHST
                     LEAGFHIIIPFVDKAAYKFSLKEAVVDIPAQICITKDNVSVEVDGLIYLEVEDAHKAA
                     YGIDNYLRAASQLAQTTLRSAIGKIDLDKTFEEREKINAEVIDAIDQAAMSWGVKVLR
                     YEIKDITPPMSVKQAMEAQMTAERQKRANIATSEGMRQAMINQSEGEKQKQINEAEGR
                     AAQVTLVAQAEARKIEMIAKATSEGIMSVAASLRGDGGMQAMNMRLAEQYITQFGNLA
                     KATNTVIMPSNVADVAGLIATAMSTVKSAK"
     gene            3836..>4264
                     /locus_tag="PKH54_09040"
     CDS             3836..>4264
                     /locus_tag="PKH54_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00081.1"
                     /translation="MRCRVFLVAIVLASVACGCGGDGSPAPEFPLSLIFEPGRPVASV
                     IDAEGSTVVRFKPGMIRLARVASSFEWDQGFLAVSRDEAAVTDFADAVATWTENAGIV
                     TATLSEDGVELAAVTFQPEQTGYLIQVGIQPGAGEVNEVRF"
BASE COUNT          858 a         1202 c         1322 g          882 t
ORIGIN      
        1 ttcttccagc atgtcgagca ggaactgctg gttgccggtt tctttccaaa cgcggacatg
       61 cgggatcaca tgatggaagt catccggggg tggctgaccc gcacgaagcc gaccgaacgc
      121 gaaatcagga tgctgcacgg tgtcctggcc acgctttccg gccgccgtct tggcgggaaa
      181 ccggcccacg tcccgggcac caagggcggc accggcgcca aggcccgcaa gcgcaggagc
      241 cagttgcctg ccgatgcccc ggaggatgcc tgattccggc ttcacgccga ttcgggaaga
      301 acctccagcc tgacctggca gtgcaggacg cccagcagtc gaagcatttg atcaaccgac
      361 ttcgtgtagt tcgtctgatc cagcaggcgg tacagctgcg ccggtgacgt tcccatcagt
      421 cgaattattt cacggcgacc catgcggctg gccttcaagt gcctcctggc ctcgatggtc
      481 aactggtaca gcagcatgtt cctgatgtag tcgggatccc ggttgtagtc gtaaacctgg
      541 tcgataagga tggagccctc gctgccgtcc ttgaggacgt aggagaatcc ggaaaacgca
      601 agctcgggat ccatgaaggc agatgacacc ggattctccg gcgtgggcga aggcgcgcaa
      661 cggcaatagg gataatcgag gctttgctgc gctccgtgca gggtgaaaca ctttttcctg
      721 ttgttgcaag tcacgctcgc cacaggagtc cctccttctg aagtctcctt atcagcgcca
      781 gggccttcaa attcagaatt ccccacatca ggcagttgtt ttccagatcc catttcatca
      841 gcaggcggcg tccgtcaaac acatggacgt ggtttggcga gtggtcgccc ttccatgcca
      901 tgaagacgaa gcccccgcga cgtatcttcg gcattatgtt accttgcagg gaaacggaat
      961 tcaagaagcc tgccggctcc aggatacata tcgcatcggt tgacgggacg tttcatgcct
     1021 gcctggcaag gtcttcccta ttgacacggc gcgccaattg cgtcgaaact gcggtgtcat
     1081 aatctggtga tcccgtcatg atgaaggtcg ttgatacccc ggaaaaaatg gttgtctccg
     1141 gtcaggcggt ctatggacgt ttcgcgactc cgttccggaa cgtgaacctg gacgatcaga
     1201 agatcgacgt gttcgggctt ccggtcccga agcccctgcg cacggcccgc ctgaaggagt
     1261 gggcacacgt ggccattgtc ggcccggaag tcacgatcgg gtgcgccgtg gtcgatgcca
     1321 agtacctttc gaacgcctgg ttctgggccg ccgatcgtgc caccgggaag ttcatcgagc
     1381 atgcggccca gtgcggtggc ggcgccatca agaccagccg ccagctttat aacggccaga
     1441 ccgatttcaa gggcggcgga ttcaaggtcg agatcgacga tcagcttgat aagaaccagc
     1501 acgtctatca catcgagatc aaggcgggaa aggaccttcc ggcaatccgc gccgatttcg
     1561 tcctgcacgc cgactggcgg aacaacaatc cgcttgtcgt ggtgcttccc gccgcgccgg
     1621 ggcgtcctct gtacacccac aagtcggtca ctccggtttc gggcatgatc gcggtcggga
     1681 accgcgtcta tcagcttgaa tccgacaagt acgtggcgat gctggatatc cagaagacct
     1741 attatcccta caggattttc tgggaatggg cgaccttcgg cggatacgac gcgaagggca
     1801 ggctgatcgg gctgaacctg tgccacaacc tgattaagga cgacgagacc tacaacgaaa
     1861 actgcctgtg ggttgaaggc aggctgcata acctggcgcc ggcgcgtttc gaatacgatt
     1921 cgtccgatta ccttaagccc tggcacctga agacggttga tggttccgtt gaccttgaat
     1981 tccgtccgga aggcaagcgg gacgggatga ccgacgcgaa ggtgctgctg tcggattatc
     2041 accagccttt cggcagtttc cacggtttcg tgaccaccga ggatggcgag cgtattgaaa
     2101 tcgacggcat ccgcggcgtg accgaagacc acaaggccaa attctgacgg acgccatccc
     2161 gatcggtgat ttccactgtc aacttgatgc catctgcgtg ataatttttc gggcttatcc
     2221 tgaactccgc gggggtcgca tatgttcgga ctcgatatgt ggcttgtctg gttccttgcc
     2281 ggatgcgtca tcgcgcttgc cgagatgatg atcccgggct tcatcatcgt gtttttcgga
     2341 cttggctgct ggggcgccgc cgtcgtcgcc gccatcgcgc cagaggcatg ggcctggcag
     2401 atcgcggcct ttcttgtcgt atcggtatcg acacttgtcg ggcttcgccg cctggccgtc
     2461 aacctgcttc ggggcaagac cggccagccg cgtggcgtgg cgtcttccgg gatcgagatt
     2521 ggaaccgtta ttttggttga tgttgaaatg ctgcctgggc gggtcggaag ggttcggtac
     2581 cgcggcacga actgggatgc aaaatcgacc gtcgcaatca cggccggctc gcaggccgta
     2641 atcgtcgggg ttgatgaaat caacaggtcc tgtcttgtca tcgaaccggt ttcggggaaa
     2701 ccggaatcca actgatattg gaggttgtgt catggccagt gcgtccatcg ttgcgcttgt
     2761 cggcttggct gtagtggtgc tggtgctcct ggtgaagggg gcaaggattg ttccacagaa
     2821 gaccgcggtc atcatcgagc ggctcgggaa gtatcacagc acgctggaag ccggtttcca
     2881 catcatcatt ccgttcgttg acaaggctgc gtacaagttc agcctgaagg aagccgtggt
     2941 cgatattccg gcgcagatct gcatcaccaa ggacaacgtc agcgtcgagg ttgacgggct
     3001 gatttacctg gaagtcgagg atgcccacaa ggctgcctac ggcatcgaca attacctgcg
     3061 tgccgcatcc cagcttgccc agacaaccct tcgttcggcg atcggaaaga tcgacctgga
     3121 caagactttc gaggaacgcg aaaagatcaa cgccgaagtc atcgacgcaa tcgatcaggc
     3181 cgccatgtcc tggggggtga aggttcttcg ttatgaaatc aaggacatca ctccgccgat
     3241 gagcgtcaag caggccatgg aggcccagat gacggccgag cggcagaagc gagcgaacat
     3301 cgccacatcc gagggcatgc gccaggcgat gatcaaccag tccgaaggcg aaaagcagaa
     3361 gcagatcaac gaggccgagg gccgtgccgc gcaggtcacg ctggttgccc aggccgaggc
     3421 cagaaagatc gagatgattg cgaaggcaac gtccgagggc atcatgtccg tcgcggcttc
     3481 cctgcgcggc gatggtggca tgcaggcaat gaacatgcgt ctggccgagc agtacatcac
     3541 ccagttcggg aatcttgcga aggcgacgaa caccgtgatc atgccaagca acgtcgcgga
     3601 tgtcgccgga ctgatcgcca ccgccatgtc caccgtcaag tccgcgaagt agagtaaagc
     3661 ccgcgaagta gagtaaagcc cgcgaagtgg tttcggtttg tcctaacctt acgctctgca
     3721 attgtttttg cggaatatgg cggaggctgt cagttgttga ccgcaagcgt gtaaccggat
     3781 aacttcgtgg tgtcggggtg atcccggcac gcattaagtt tccatggagg ttgcaatgag
     3841 gtgccgtgtc ttcctggtgg cgatcgttct cgcctcggtg gcctgtggat gtggcggcga
     3901 cgggtcaccc gcccctgaat tcccgctttc gttgattttc gagccgggca ggccggtcgc
     3961 ctcggtaatc gacgccgaag gctcgacggt ggtgcgcttc aagccgggca tgatccggct
     4021 ggccagggtc gcgagttcat tcgagtggga tcagggtttc ctggccgtga gtcgggacga
     4081 agccgccgtg acggatttcg cggatgcggt tgccacatgg acggaaaacg ccggtatcgt
     4141 gaccgccacg ctgtccgaag acggcgttga actggcggcg gtgaccttcc agccggagca
     4201 gaccgggtac ctgattcagg tcgggatcca gcctggggcg ggggaggtca acgaggttcg
     4261 cttc
//
LOCUS       DAOPPA010000279         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22636_length_4246_cov_4.626819, whole genome shotgun sequence.
ACCESSION   DAOPPA010000279 DAOPPA010000000
VERSION     DAOPPA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22636_length_4246_cov_4.626819"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /gene="gatE"
                     /locus_tag="PKH54_09045"
     CDS             complement(<1..1438)
                     /gene="gatE"
                     /locus_tag="PKH54_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOD00082.1"
                     /translation="MKKPGIQFAEPEFYEKLGLICGIEMHRQLHTEQKLFCRCPVRPY
                     SNVYHAEILRHMRPTLSELGEYDPTALMEFKTKKEVLYRLNRDTVCTYEIDDAPPFSL
                     NREALNKAIRMALILNLSLVNEIHIARKQYLDGSIPTGFQRTTILGVGGYMMVDGTRI
                     GIRQLGLEEDACREVSDVAHRRTYLTDRLSIPLIEIVTEADMKTPAQAARVANAIRRT
                     CHLSQVCRFGYGRARQDVNVSIRGGERVEIKGVPSIRQIPALVHYEALRQKALLEIRD
                     RLLRDGIAGDSFDTVVDISDLVRNHPHPAFATRWGAGVSASAIILRRCAGILAYPTGP
                     GRTFGSEVADRIKVVACIDRMPNCLSSDNPEAFEYADLFAKVRELGGADNNDCVVMVA
                     GPRADVETAMKEVRLRMQEALVGVPRETRRSLRNGGTGFERLLPGPNRMYPDTDLPPI
                     VLTDADFARERALCPQPLWEKEDAWKAMG"
     gene            complement(1491..2270)
                     /gene="tsaB"
                     /locus_tag="PKH54_09050"
     CDS             complement(1491..2270)
                     /gene="tsaB"
                     /locus_tag="PKH54_09050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOD00083.1"
                     /translation="MTDKPDIRQLRSAKKRRSAETGHARPVIIACDTSTTIGSVAIVE
                     GGAVTAETVVKSLNSHSLRILKDIEALLGHRELTMNDVDAFATAAGPGSFTGVRIAMS
                     TLKGLAWSLGKPLIPVGSLHALAWPLLGRGAPVLAAFDAKRSEVYGALFGPDGKPLVE
                     QAALAPNDLARACLVAVEDGGPIICVGEGVLEYHDVFKAALGDRLAMAGAWEAYVRAS
                     VIGIIGTAELAAGRICDVATVEPVYLRRSEAEINLSKTSIN"
     gene            complement(2280..3968)
                     /locus_tag="PKH54_09055"
     CDS             complement(2280..3968)
                     /locus_tag="PKH54_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD00084.1"
                     /translation="MSVLYFILLLGVLIFIHELGHYLAARAVGVTVTKFSLGFGPKII
                     GFTRGGTEFLLSAVPLGGYCKFMGDDPENLPAFDDRKKGFLTTDIWRRTVIVLAGPVF
                     NLVLPLVAFMPGALSETTLPPAIIGTASIGGAAWEGGLRPGDRLLAINGSAVRYWQDM
                     VDVVSANPEKDLKVVVRREPVAGLDGRPGPSFHNLTVRPKKILDPTYRQLGFEKALGR
                     IEASLDRPLPVIMVRQGSPAQLAGLKDFDAVSSVDGVKVWSFAELMQKLREKAGRQVT
                     LEVSSIDDQAAEAGSVSARQILMTLPPQAAPEGSATAADAVGTAGEDAGFSDGSFVIL
                     EVENDSAAAAAGLAQNDLIVALNGREYNDPGFLISELSQDMDRDHLFTVRRGTTTSTL
                     KLNLKNPEWQPGSALPKYLSAGFKVRRVTVQPEYIPNDNLIGWAFVQTFKRTADVFVG
                     TVASVAALFTGRVSFKQMGGPIMIYDLAASAGKQGVMAFLSVLGWLSVSLGVLNLLPI
                     PILDGGHLVLFAYEGIRRRPPTMKERTIWSWAGLIILVLIMAAVMKNDIMRKIG"
BASE COUNT          785 a         1329 c         1287 g          845 t
ORIGIN      
        1 ctcccatggc cttccaggcg tcttcctttt cccacagcgg ctgcgggcac agcgcgcgct
       61 cgcgggcgaa gtccgcgtcg gtcaggacaa tcggcggcag gtcggtgtcg ggatacatgc
      121 ggttcgggcc gggcagcagg cgttcgaacc cggtgccacc gttgcgcagc gaccgtctgg
      181 tttcacgcgg cacgccgacc agggcctcct gcatgcgcag gcggacttcc ttcatcgcgg
      241 tttccacgtc cgcgcgcggc ccggccacca tcaccacgca gtcgttgttg tccgcgccgc
      301 ccagttcgcg aaccttcgcg aaaaggtcgg catattcgaa tgcttccggg ttgtccgagc
      361 tcaggcagtt cggcatgcgg tcgatgcacg cgacgacctt gattcggtcg gcgacttccg
      421 atccgaacgt gcggccgggg cctgtcgggt aagccaggat gccggcgcag cggcgcagga
      481 tgatggcgga tgccgaaacc ccggcgcccc atctggtcgc gaaagccgga tgcggatggt
      541 tgcgaaccag gtcgctgata tcgacgaccg tgtcgaacga atctccggcg atgccgtcgc
      601 gcagcagcct gtcgcggatt tccagcaggg ccttctgtcg aagcgcctcg taatgaacca
      661 gggccgggat ctggcggatg gacggaaccc ccttgatctc gacgcgttcc ccgcccctga
      721 tcgaaacgtt gacgtcctgg cgggccctgc cgtatccgaa gcggcacacc tgcgacaggt
      781 ggcacgtgcg gcggatcgcg ttcgcgaccc tggccgcctg cgccggcgtc ttcatgtcgg
      841 cctcggtgac gatttcgata agcggaatcg acagccgatc cgtcaggtaa gtcctgcggt
      901 gcgcgacgtc cgacacctcg cggcaggcat cttcttccag gccaagctgc ctgatgccga
      961 tgcgcgttcc gtcaaccatc atgtatccgc cgacgcccag gatcgtggtt cgctggaacc
     1021 cggtcgggat cgacccgtcc aggtactgct tgcgcgcgat atggatttcg ttgaccaggg
     1081 aaaggttcag gatcagggcc atcctgatgg ccttgttcaa tgcctcgcgg ttcagcgaga
     1141 acggcggcgc gtcgtcgatt tcgtaagtgc agacggtgtc cctgttcagg cggtaaagga
     1201 cttccttttt cgtcttgaat tccatcaggg ccgtcgggtc gtattcgccc agttcggaca
     1261 gcgtcggccg catgtgccgc aggatttcgg cgtgatagac gttgctgtac gggcggacag
     1321 ggcaccggca gaaaagcttc tgctcggtgt gaagctgcct gtgcatctcg atgccacaaa
     1381 tcagtccgag tttttcgtaa aactcgggct cagcgaactg gattccgggc ttcttcatgg
     1441 aaagtctccg tctgcacggt tgcaatggaa cctggccggc gcggatggta tcaatttatg
     1501 gatgttttgg acaggttgat ttcggcttcc gagcgtcgca gatagaccgg ttcgacggtg
     1561 gccacgtcgc agattcttcc ggccgccagt tcggccgtcc cgattattcc gatgaccgat
     1621 gcgcggacgt acgcttccca ggcgccggcc attgccagtc tgtcgccaag tgccgccttg
     1681 aacacgtcat ggtattcaag aacgccctca ccgacgcata taatcgggcc gccgtcctcg
     1741 accgccacca ggcacgcacg cgcaaggtcg ttcggtgcaa gcgccgcctg ctcgacaagc
     1801 ggctttccgt ccggcccgaa caaggcacca taaacttcac tgcgttttgc gtcgaatgcg
     1861 gcaagtacgg gcgccccgcg tcccagaagc ggccacgcca gcgcgtgaag cgaaccgacg
     1921 ggaatcagcg gctttccaag gctccaggcc agacccttca acgttgacat cgcaatccgg
     1981 acgccggtga acgaacctgg acccgccgcg gtcgcgaacg catcgacatc gttcatcgtc
     2041 aattcgcggt ggccaagcag ggcctcgatg tccttcagga ttcgaaggct gtggctgtta
     2101 agggacttga cgaccgtttc agcggtaacc gccccgcctt ccacgatggc gaccgacccg
     2161 atggtggtcg atgtgtcgca ggcaatgatt accggccgcg catgtccggt ttcggcggat
     2221 cgccgcttct tcgcgcttcg aagctgcctg atgtcaggtt tgtccgtcat tgccgccccc
     2281 tagccaatct tgcgcatgat gtcgttcttc atgacggcgg ccatgataag caccagaata
     2341 atcaggccgg cccaggacca gatggtcctt tccttcatgg tcggtggtct tcgcctgatg
     2401 ccttcatagg cgaaaaggac aaggtgtccg ccgtccagga tcgggatggg cagcaggttc
     2461 agcactccaa ggctgactga aagccatccg agcacgctca ggaaggccat cacgccctgc
     2521 tttccggcgg atgcggccag gtcgtaaatc atgatcgggc cgcccatctg cttgaacgaa
     2581 acccggcccg tgaagagcgc ggccacgctt gcgaccgttc cgacgaacac gtccgccgtg
     2641 cgtttgaacg tctggacgaa ggcccagcca atcaggttgt cgttcgggat gtattcgggc
     2701 tgaaccgtca cgcgacggac cttgaacccg gccgacaggt acttcggcag tgccgatccc
     2761 ggctgccatt ccgggttttt caggttcagc ttcagcgttg acgtggtggt tcccctgcgg
     2821 accgtaaaca ggtggtcgcg atccatgtcc tgggaaagct cggaaatcag gaatcccggg
     2881 tcgttgtatt cccgtccgtt cagggccacg attaagtcgt tctgggccag gccggccgcc
     2941 gccgccgcgc tgtcgttttc aacctcaagg attacgaatg agccgtcgct gaacccggcg
     3001 tcttccccgg ccgtcccgac agcgtcggcc gccgttgccg aaccttccgg tgcggcctgc
     3061 ggcggcaatg tcatcaagat ttggcgcgca gaaacggatc ccgcttcggc agcctgatcg
     3121 tcaattgacg atacctccag ggtcacctgg cgtcccgcct tttcgcgcag tttctgcatg
     3181 agttcggcaa acgaccatac cttgaccccg tcaaccgaac tgacagcatc gaagtccttc
     3241 aagccggcca actgcgcggg gcttccctgc ctgaccatta tgaccggcag aggtctgtcc
     3301 agagatgcct cgatacgccc cagcgccttc tcaaaaccga gttgcctgta cgtcggatcc
     3361 aggattttct tcggccttac ggtcaggttg tgaaatgacg gccccggcct gccgtccagc
     3421 ccggcaaccg gctccctgcg aaccacgact ttcagatcct tttcggggtt tgcgcttacg
     3481 acatcgacca tgtcctgcca gtaacggacg gcgcttccgt tgattgcaag aagccggtca
     3541 cctggccgca ggccgccttc ccacgcggcg cccccgattg aagcggttcc gatgattgcc
     3601 ggtggcaggg ttgtttccga aagggcgccc ggcatgaacg cgacaagtgg cagaaccagg
     3661 ttgaagacgg gcccggcaag gacgatcacc gtccttctcc agatatccgt cgtaagaaat
     3721 ccctttttcc tgtcgtcaaa tgcgggcagg ttttccgggt cgtcacccat gaacttgcag
     3781 tatccgccaa gcggcacggc ggaaagcagg aattcggttc cgcccctggt gaacccgata
     3841 attttcggtc cgaagccaag cgaaaacttg gtcacggtga cgccgaccgc gcgggcggcc
     3901 aggtagtgcc cgagttcgtg aatgaatatc aggacgccaa ggagcaatat gaaatacagg
     3961 acgctcataa tcaaaatccc gcgaaaaacc ggacgcagga tagcagacca tgaacgcggg
     4021 cgccatttcc ggccagtcga ttggcgaaat tacaaagtct tgcaacgcgt cggttgcggg
     4081 ttacgggatt caggtcgagg gcggtccgtc cggtctatct gggaaaggag cccacgcgtt
     4141 cgattcggcc gaaaggcccg tttccggcag accacaggac gaacagcggc tggcccgcga
     4201 tgtttgaagt gaatacgggg ccgatgaacc tggaatccgt cgaatg
//
LOCUS       DAOPPA010000280         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22685_length_4237_cov_4.060497, whole genome shotgun sequence.
ACCESSION   DAOPPA010000280 DAOPPA010000000
VERSION     DAOPPA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22685_length_4237_cov_4.060497"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="hydG"
                     /locus_tag="PKH54_09060"
     CDS             <1..929
                     /gene="hydG"
                     /locus_tag="PKH54_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOD00085.1"
                     /translation="NKNGEIRRVNMNCIPLDVEGYRKMKKVGIGTYQIFQETYHLPTY
                     KVMHPHGPKSDFLWRLYGQDRAMEGGCDDVGIGALFGLYDWRFEALGMLAHTIHLEER
                     YHVGPHTFSFPRIKDASNRAFDPQWAVSDHDFKRLIAILRLSVPYVGMILTAREPAKL
                     RRELIDFGCSQIDAGTRIGIGAYSCADEEQDLKKEQFRIGDCRPLDEVVGELTDMNEI
                     PSWCTACYRKGRTGEHFMEFAIPGFIKRFCTPNALMTLKEYIVDYASPEVKEKGEKLI
                     ADELAKMPEGDQKTELIARLERIEKGERDLQF"
     gene            complement(926..1282)
                     /locus_tag="PKH54_09065"
     CDS             complement(926..1282)
                     /locus_tag="PKH54_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD00086.1"
                     /translation="MMNQDASRTPPSFTIRLTREAQDDVAQIVDFLFMREGRGTADRI
                     LDGLEETIHSLAQHPNRGSRPPELAEIGVREFREVFFKPYRIIYRVFPDQIVVMLVSD
                     GRRDMRTLLSRRMLAP"
     gene            complement(1279..1560)
                     /locus_tag="PKH54_09070"
     CDS             complement(1279..1560)
                     /locus_tag="PKH54_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOD00087.1"
                     /translation="MKYSNQIKPISYLKAHAAELIREINKSGEPLIVTQNGEAKVVVQ
                     DITKYDQEQETLALLKILALGMRQVEEGKVVPFEEVFDELRADLEGRIE"
     gene            1755..>2397
                     /locus_tag="PKH54_09075"
     CDS             1755..>2397
                     /locus_tag="PKH54_09075"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOD00088.1"
                     /translation="MTNRLRVAFSAVLLVAGCDEGIVEPVAPYCEDDVRVEVVDGQLR
                     RTECGDGSFCNAGECVATDVKFPADVGFHDDRCEWWYYTGHVAGSGREFGFEVTIFQY
                     DYEVFFGVPGVGYMCHVAVLDATAGVHFHNDAIGLSTNEWTNEPTVLDVDNCRFEMDG
                     FGNDHIVGVIPEGMEKNGLASPWVIDLIVTPQKRATLHGGDGFIPMADTGGTSY"
     assembly_gap    2398..2407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2408..2920
                     /locus_tag="PKH54_09080"
     CDS             <2408..2920
                     /locus_tag="PKH54_09080"
                     /inference="COORDINATES: protein motif:HMM:NF028495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOD00089.1"
                     /translation="AAEGTISTPDGDVEVTGQAWMDHQWGQFDMVDFKGWDWWSIQLD
                     DNREIMLFQFTDWDGVLCEQAGTIIEADGTATELHGFDDFHITPKRTWTSPWTNGVYP
                     LDWDITIAKGDWNLQVLTHVDDQEMFNIAQNYWEGATTITGTNGGLPVTGVGYTELTG
                     YAKDDLDPVR"
     gene            3006..3854
                     /locus_tag="PKH54_09085"
     CDS             3006..3854
                     /locus_tag="PKH54_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOD00090.1"
                     /translation="MLTILQVEARNTKSVIMWVRDFLATHPVFTVGDFQQAHREQGSA
                     NVQTFNSLLAYHVRQGNIVRIRRGLLAAVPYGYTPETVPVDPFLVTMGLSDDAVVAFH
                     AALQFHGRAHTIHNRYQYLTAARRRPFTFRGQDFVPVTGGVPGWHTGIDHGVVEERNA
                     GGTVRVTTLERTLVDVISLPQFGGGWEEVFMSLDSIEFLDVDRVVEDAVRRDSLTVAR
                     TGFYLETRKGDLPIEEIHLAALQERSPRQPSYMGPGRQRGGRLVKRWNLVVPGAILDR
                     TWEEPD"
     gene            3851..>4237
                     /locus_tag="PKH54_09090"
     CDS             3851..>4237
                     /locus_tag="PKH54_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD00091.1"
                     /translation="MNLTREDVRKVAGTTGFPEEALEKSLRLLGVLKALSGYRGLEGR
                     LVLKCGTALNLFLLRIPRLSVDIDLNYIGSANRDAMMIERPLVERAIEGICQREGFVP
                     RRVPGAHAGGKWRMAYRDVNGRNSHLS"
BASE COUNT          897 a         1146 c         1279 g          905 t
ORIGIN      
        1 agaacaagaa cggcgagatc cggcgcgtca acatgaactg catcccgctg gacgtggaag
       61 gctatcggaa gatgaaaaag gtcggcatcg gcacgtacca gatcttccag gaaacgtacc
      121 atctgccgac atacaaggtg atgcacccgc atgggccgaa gtccgatttc ctgtggcgcc
      181 tgtacggcca ggatcgtgcc atggaaggcg gctgcgacga cgtcggcatc ggggcacttt
      241 tcggccttta cgactggcgt ttcgaggcac tggggatgct ggcgcacacc atccacctgg
      301 aagagcgtta tcacgtcggc ccgcacactt tcagcttccc gcgcatcaag gatgccagca
      361 accgcgcgtt cgacccgcag tgggccgttt ccgatcacga cttcaagcgc ctgattgcga
      421 tactgcgcct gtcggtcccg tacgtcggca tgatccttac ggcgcgtgaa ccagccaagc
      481 tgcggcgcga gttgatcgat ttcggatgca gccagatcga cgccggcacg cggatcggca
      541 tcggagccta ttcatgtgca gacgaagaac aggaccttaa aaaagagcag ttccgcatcg
      601 gcgactgccg tcccctggac gaggtcgtcg gcgagttgac cgacatgaac gagatcccgt
      661 cgtggtgcac cgcgtgctat cgcaagggcc gcaccggcga gcatttcatg gaattcgcga
      721 ttcccggttt catcaagcgc ttctgcactc cgaacgcgtt gatgacgtta aaggaataca
      781 tcgtcgatta cgcgtccccg gaagtgaagg aaaagggcga aaagctgatc gccgacgaat
      841 tggcgaagat gcctgaaggt gaccagaaga ccgaattgat tgcccgcctg gaaagaatcg
      901 aaaagggtga acgcgacctg cagttctagg gcgccagcat cctgcgcgac agcagggtcc
      961 tcatgtcacg gcggccatcc gaaacaagca tcacgacaat ctgatccggg aacactcgat
     1021 agattatccg gtacggtttg aaaaacactt cccggaattc cctgacgccg atttcagcca
     1081 gttcgggcgg gcgggaacca cggtttgggt gttgtgcaag ggaatggatt gtttcttcaa
     1141 ggccgtccag aatcctgtcg gcggtgcccc gaccttcgcg catgaacaaa aaatcgacta
     1201 tctgtgcgac atcgtcctgg gcttccctgg taagcctgat tgtgaaactg ggtggagtcc
     1261 tggatgcatc ctgattcatc attcgatcct gccttcaaga tcggcccgga gttcatcgaa
     1321 cacctcttca aacgggacaa cctttccttc ctcgacctgg cgcatgccca gcgcaaggat
     1381 cttcagcagg gccagcgttt cctgttcctg gtcgtacttc gtgatgtcct ggacgaccac
     1441 ttttgcttcc ccgttctgcg tgacgatcag gggttcgccg gacttgttga tttcgcggat
     1501 aagctcggct gcgtgtgcct tcaggtagct tatcggcttg atctggttcg agtacttcat
     1561 ggcagcaccg aagcatgtga cagatcttaa tttagtctgc aaataggtca tgagtcaacc
     1621 tctgatctcg ggtggcgggt tactgcgaag tatcggtatg cgtaaagaaa gtgtttcacc
     1681 gtgttgatgc ctgaagtctt gtaaccttcc ggacgaaaat cgataatggc gcatcgtttg
     1741 gatgcgggcg tggcatgacc aacagacttc gtgtggcctt ttccgcggtg cttcttgtgg
     1801 ccggatgtga cgaaggcatc gtcgagccgg tggccccgta ctgcgaggac gacgtgcgcg
     1861 tcgaagtcgt ggacggccag cttcggcgca ccgaatgcgg cgacggatca ttctgcaacg
     1921 cgggtgaatg cgtcgcgaca gacgtgaagt tcccggccga cgtcgggttc cacgatgacc
     1981 gttgcgagtg gtggtactac accggccacg ttgccggttc cggccgggaa ttcggctttg
     2041 aggtcacgat ttttcaatac gactacgaag tgttcttcgg tgttccaggc gtcggctaca
     2101 tgtgccatgt tgcggtgctg gatgcaacgg cgggcgtcca cttccacaac gatgccatcg
     2161 gcctgagtac aaacgaatgg acgaacgagc cgaccgtgct ggatgtcgat aactgccggt
     2221 tcgagatgga cggtttcggc aacgaccata tcgtcggcgt gattccggaa ggcatggaaa
     2281 agaacggcct ggcctcgcca tgggtgatcg acctgatcgt gacgccgcag aagcgcgcga
     2341 cgctgcacgg cggggacggt ttcataccga tggccgacac cggcggaacc agctattnnn
     2401 nnnnnnngcg gccgaaggca cgatttcgac accggacggc gacgtggaag tgaccggaca
     2461 ggcatggatg gaccaccagt ggggccagtt cgacatggtg gatttcaagg gttgggactg
     2521 gtggagcatc caactcgacg acaaccgcga aatcatgctt ttccagttca cggactggga
     2581 cggcgtcctg tgcgagcagg ccggcaccat catcgaggcc gacgggacgg cgaccgaact
     2641 ccacggcttt gacgatttcc acattacacc gaagcgcact tggacaagcc cgtggacaaa
     2701 tggcgtctat cccctggact gggacatcac cattgccaag ggcgactgga accttcaggt
     2761 cctgacgcac gtcgatgacc aggagatgtt caacatcgcc cagaactact gggaaggcgc
     2821 cacgaccatc acgggaacaa acggtgggct tccggttacc ggcgtcggct ataccgagct
     2881 gaccggctac gccaaggacg acctggatcc ggtcaggtaa tcagatctca agcacgtgat
     2941 catggtgcgg tcgccttgca gcaaagctaa gtaacatctc cctgacttga cttcaagtac
     3001 cgttgttgtt aacaatatta caagttgaag cgcgtaacac aaagtcggtt atcatgtggg
     3061 tacgagattt tctggcaaca cacccggtgt tcaccgtggg tgattttcag caagcccatc
     3121 gggaacaagg ctccgccaac gtgcagacct tcaattcttt gctggcctat cacgttcggc
     3181 agggcaatat cgttcgcata aggcggggat tgctggctgc ggttccttat ggatacacgc
     3241 cagaaaccgt gcccgttgat ccctttctgg tcactatggg cctttctgac gacgctgttg
     3301 tggcctttca cgcggcgctg cagtttcatg ggcgtgccca tacgattcac aaccgatacc
     3361 agtatctgac cgcagccagg cggcgtccat tcacctttcg tggccaggat ttcgtgcccg
     3421 tgaccggagg cgttccgggc tggcatacgg gaatcgacca cggcgtcgta gaagaacgaa
     3481 atgctggggg caccgttcga gtcactacct tggaaaggac tctggtggat gtgatatccc
     3541 tgccccaatt cggaggcgga tgggaagaag tttttatgtc cctggactcg atcgaattcc
     3601 tggatgtcga tcgggttgtc gaggatgccg tgcggcgtga ttccctgaca gtggcgcgta
     3661 ccggatttta tctggaaacc cgcaaagggg atcttccgat tgaagaaatt catctggcgg
     3721 ctcttcagga acgttcgccc agacaaccca gctatatggg gcccggtcgt caacgtggcg
     3781 gcaggctggt gaagaggtgg aatcttgtgg tgcctggggc aatccttgat cgaacgtggg
     3841 aggaaccgga ttgaatctga cgcgcgaaga cgttcggaag gttgccggga caaccggatt
     3901 ccccgaggaa gcccttgaga agtcccttcg tctgcttggt gtgctcaagg cgttgtcagg
     3961 gtatcgggga ctcgagggac gccttgtatt gaagtgtggc acggcgctga atcttttcct
     4021 gctgcgaatt ccacggcttt cggttgatat tgacctgaat tacatcggat cggccaaccg
     4081 ggatgcgatg atgattgaac ggcctctggt tgagcgggcc attgagggga tatgtcagcg
     4141 cgagggcttc gtgccgcgac gtgttcccgg cgcccatgct ggcggaaagt ggcggatggc
     4201 atacagggat gtcaacggtc gaaacagtca tctgtcc
//
LOCUS       DAOPPA010000281         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22710_length_4232_cov_3.428777, whole genome shotgun sequence.
ACCESSION   DAOPPA010000281 DAOPPA010000000
VERSION     DAOPPA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22710_length_4232_cov_3.428777"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKH54_09095"
     CDS             complement(<1..439)
                     /locus_tag="PKH54_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOD00092.1"
                     /translation="MADQINKPEELAGEEDFASMLDNYEQHEVKDGEIVTGTVQRVEK
                     DWVLIDIGYKSEGYVPLHEFMDENGNITVKEGDQTEVYVESRENREGYLELSKDKAEK
                     LKIWDDISVAAERDGVVEGIVLSRVKGGLTVDIGVKAFLPGSQI"
     gene            complement(597..1634)
                     /locus_tag="PKH54_09100"
     CDS             complement(597..1634)
                     /locus_tag="PKH54_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00093.1"
                     /translation="MHRLFQAGIAGLTITILMIGGTTGCEDADVPTTGYFGRVGTSGC
                     VPTVCQVSGDPLSERKAWHVAHPCEPMPDELACRVRTVLDGEDWPDKVEEAIETASAE
                     DESLTDLQKEALRCLAFMPGVNQFAIGRIRDGSPRQISLNMPVVLQGGNRLAAQEYID
                     RFGPYLGQIWNLNRKLSLQTTVSGDIIITTSVNAYDGIELRYPQSMEIRLDRFQQAPT
                     WEGCPNLYTNTVIDSQIVSGTIKTGEGGRLTPEQATRHVIESHPDCVELPESAPTLVI
                     WNSRLYYMLTVGCKSHCGDPGWPFEAVVDAYTGVVVDFNEECCVDCWEEDAKAFPEQD
                     AAQPEDDIPTA"
     gene            1817..3499
                     /locus_tag="PKH54_09105"
     CDS             1817..3499
                     /locus_tag="PKH54_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00094.1"
                     /translation="MKKFFSLVAVFAFSSIVIACGDSNNQPDTSGDDVNVNDVSDIAQ
                     PDVPEDTGNDVRPDVPDPDVVDPDTVDPDVVPEIVDDVVEDLGRPDVIQYDGFYVPNP
                     IDPYEDVLPKDVDWQSLPKMPAGKTFTTRYAAGVGITSITPDFVVYLGGFGNCTANDD
                     GCRKTDLVHDPVEVRAVAFADTTTNDIVIMVGIDNVGLLDFDVVGAHRHVQKKLYESF
                     GIHFPGANAILAFSHAHSSIDTTGLWGPMMGAGRDEAYTAMVLSQIAEACRLAVEDLG
                     DIELSWGVDELLENYSGDPDTLDSNMWVIKGVKPGETPELKFTLTRWAAHPTTYGDDY
                     LTISSDFPGTFRYAMERDFGGKSIYINGPLGDTYPNRPGECGLEEEFFPEGDRAPGME
                     EGDGYMKATCTGLMVANAAQGVLQTLTPLAETGIEVKHDQVFFHPYNDFLMFAIGNIP
                     LPFPTCEAIMDDCYIYMRYSLVRIGDLTFITAPGEIFPTFAKDLTDIAATAGYPNPMV
                     VFGQGWLGYLMTEAHYNDASLKDLDYHRGLCPGPDLYPKFIESLTKMVTPAE"
     gene            3634..>4232
                     /locus_tag="PKH54_09110"
     CDS             3634..>4232
                     /locus_tag="PKH54_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOD00095.1"
                     /translation="MSDDVKDTGITEDTVAVARPLNFIEEIIEEDLRTNKHGGRVATR
                     FPPEPNGYLHIGHAKAICVDFGNAQKYGGTCNLRFDDTNPTREDVEYVDSIKEDIAWL
                     GFSWSGMYYASDYFEQIYQWAELLIQKGLAYVDESTEEELREYKGNFYTKGRPTKYRD
                     RPIEESLDLFRRMRAGEFADGAMVLRAKIDLESQNMNMR"
BASE COUNT          863 a         1251 c         1219 g          899 t
ORIGIN      
        1 cgatctggga accgggcagg aacgccttga cgccgatatc gacagtcagg ccgcccttga
       61 ccctggacag gacgatacct tcaacgacgc cgtcacgctc ggcggccacg ctgatgtcgt
      121 cccagatctt cagcttttca gccttgtcct tgctgagttc caggtatcct tcacggtttt
      181 cgcggctttc gacgtaaact tccgtctgat cgccttcctt gacggtgatg ttgccgttct
      241 cgtccatgaa ttcatgaagc ggaacgtaac cttcactctt gtaaccgatg tcgatcagga
      301 cccagtcctt ttcgaccctc tggacggtgc cggttacgat ctctccgtcc ttgacctcgt
      361 gctgttcgta gttgtccagc attgaagcga agtcttcttc gcccgccagc tcttccggct
      421 tgttgatctg atccgccatt caaggcctcc cttgttttcg gcccgcccaa tattttttat
      481 ttaggcacct gcgggcctac ccggcgcgta acacgcgtcg cgggcgagta gtatatcttt
      541 tgattggcat gggtcaagtg ggaagtcggg gttttattgc ggtttttccc tttcgttcac
      601 gctgtcggga tgtcgtcctc cggctgggct gcgtcctgct ccgggaaggc cttcgcatcc
      661 tcttcccagc agtcaacgca gcattcctcg ttgaaatcaa ccacgacgcc ggtgtacgca
      721 tcgaccacgg cttcgaaggg ccagcccggg tcgccgcagt gtgatttgca cccgacggtc
      781 agcatgtagt aaaggcggga gttccagatg acaagggtcg gcgcggattc gggaagttcg
      841 acacagtccg gatgggattc gattacatgc cgggtggcct gttcaggggt cagcctgccg
      901 ccttcgcccg tcttgattgt gccggaaacg atttgcgagt cgattacggt atttgtgtaa
      961 aggttcgggc agccttccca cgtcggcgcc tgttggaatc gatcaagccg gatttccatg
     1021 ctctgcgggt atcgaagttc aatgccatcg tacgcgttga cgctggtggt gatgatgatg
     1081 tcccctgaaa cagtggtttg aagggacaac ttccggttca ggttccagat ctggcccagg
     1141 taaggcccga agcgatcgat gtactcctgc gccgccagac ggttgccgcc ctgaagcacg
     1201 acgggcatgt tcagcgagat ctgccggggc gagccgtccc tgatgcggcc gatggcaaac
     1261 tggttgacac cgggcatgaa tgccaggcaa cgcagtgcct ctttctgaag gtctgtaagg
     1321 ctttcgtctt ccgccgaggc ggtctcaatc gcttcctcga ccttgtccgg ccagtcttca
     1381 ccatccagga cggtgcgcac ccggcaggca agctcgtccg gcatcggctc gcacggatgg
     1441 gcgacatgcc acgccttcct ttccgacaac gggtcacccg atacctggca gacggtcggc
     1501 acgcatccgg acgtaccgac ccggccgaag taaccggttg tcgggacatc cgcatcctcg
     1561 caaccggttg ttcccccaat catcaggatt gtgatggtca gcccagcgat tccggcctga
     1621 aacaaacggt gcatcgaagg accccgtaaa agtgcgcaac cattttagca aaagcattgt
     1681 cttgtatcca atggccatat gagaaaatgt tttgttcaat gactatggca aattgagggt
     1741 acaggacgta tattctgacg gcccctgtcg tgaagacggg cgatttcctg aaaccaagat
     1801 tccgggagtg attcagatga agaaattttt cagtcttgtc gcagtgttcg cgttttcgtc
     1861 catcgtgatt gcatgtggcg acagcaacaa tcagccagac acgtctggtg acgacgtcaa
     1921 cgtcaacgac gtgtccgaca tcgcgcagcc ggacgttccg gaagataccg gcaatgacgt
     1981 tcgcccggat gttcccgatc ccgacgtggt cgatcccgat acggtcgatc ccgacgtggt
     2041 tccggaaatc gtcgatgacg ttgtcgagga tctgggaagg ccggacgtga ttcagtacga
     2101 cggcttctat gtcccgaacc cgatcgatcc ctatgaagac gtgctgccga aggacgttga
     2161 ctggcagtcg ctgccgaaga tgccggccgg caagacgttc acgacccggt acgccgccgg
     2221 cgtcgggatt acgtcgatca cccccgattt cgtcgtttat ctgggcggct tcggcaactg
     2281 caccgccaat gacgacggct gccgcaagac cgacctggta catgacccgg tggaagtccg
     2341 tgcggttgcc ttcgcggaca ccaccacgaa cgatattgtc atcatggtcg gcatcgataa
     2401 cgtcggcctg ctcgacttcg acgttgtcgg cgctcatcgc catgtccaga agaagctgta
     2461 cgagtcgttc gggattcatt tcccgggcgc caacgccatc ctggcatttt cccacgcgca
     2521 cagttcgatt gacacgaccg gcctgtgggg cccgatgatg ggcgccggcc gtgacgaggc
     2581 atacaccgcc atggtgcttt cccagatcgc ggaagcctgt cgcctggcgg tcgaggatct
     2641 tggcgacatc gaactgagct ggggcgtcga tgaactgctg gaaaattaca gcggcgaccc
     2701 cgataccctg gacagcaaca tgtgggtcat caagggcgtg aagcccggtg aaaccccgga
     2761 actgaagttc acgctgacac gctgggccgc acacccgaca acgtacggtg atgattatct
     2821 gaccatttcg tcggacttcc ccggcacgtt ccgttacgcg atggagcgcg atttcggcgg
     2881 caagtcgatt tacatcaacg gaccgcttgg cgacacgtat ccgaaccgtc ccggcgagtg
     2941 cggcctggaa gaggaattct tccccgaagg cgaccgcgca cccggcatgg aagaaggtga
     3001 cggatacatg aaggccacgt gcaccggcct gatggtcgcg aacgcggccc agggcgtgct
     3061 gcaaacgttg actccgctgg ccgaaaccgg catcgaggtc aagcacgacc aggtgttctt
     3121 ccacccgtac aacgacttcc tgatgttcgc catcggcaat attccgttgc ccttcccgac
     3181 ctgcgaagcc atcatggacg attgttacat ctatatgcgg tactcgctgg tgcgcatcgg
     3241 cgatctgacg tttatcacgg cccccggcga aatcttcccg acgttcgcca aggacctgac
     3301 cgacatcgcg gccaccgccg gctacccgaa cccgatggtc gtgttcggcc agggctggct
     3361 gggctacctg atgacggaag cgcattacaa cgatgcgtcg ctgaaggatc tggactacca
     3421 ccgcggcctg tgccccggcc cggaccttta ccccaagttc atcgaaagcc tgacgaagat
     3481 ggtcaccccc gccgagtagt aatcgtcaat tccggttcat tacgggcgga ttacatccgc
     3541 ccgctgcggt tcaaacctta cgcttgcatc aagaccagcc cggcgtaaaa ttcacgcggc
     3601 gaaatgttcc aaccaggggt tgtggggata atgatgtctg atgacgtgaa agataccgga
     3661 ataactgaag atacggtcgc agtggccagg ccgctgaact tcatcgagga aatcatcgag
     3721 gaagacctgc ggacgaacaa gcacggtgga agggtcgcga cccgcttccc gccggaaccg
     3781 aacggctatc ttcacatcgg ccacgccaag gcgatctgcg tcgattttgg caacgcccag
     3841 aagtacggcg gaacctgcaa cctgcgtttc gacgacacca acccgaccag ggaagacgtc
     3901 gagtacgtcg attcgatcaa ggaagatatc gcatggctgg gcttttcgtg gtccggcatg
     3961 tattacgcgt ccgattattt cgagcagatc taccagtggg cggaactgct gatccagaag
     4021 ggcctggcgt acgtcgatga atcgactgaa gaggaacttc gggaatacaa aggtaatttt
     4081 tacacgaagg gccgccccac gaagtaccgg gaccgcccga tcgaggaaag cctggacctg
     4141 ttccgccgca tgcgtgccgg cgagttcgcg gacggcgcga tggtcctgcg ggcgaaaatc
     4201 gacctggaat cccagaacat gaacatgcgc ga
//
LOCUS       DAOPPA010000282         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22725_length_4230_cov_3.710898, whole genome shotgun sequence.
ACCESSION   DAOPPA010000282 DAOPPA010000000
VERSION     DAOPPA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22725_length_4230_cov_3.710898"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKH54_09115"
     CDS             <1..802
                     /locus_tag="PKH54_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00096.1"
                     /translation="TGECKPGCSGDKPICNLETRQCEPQNCKGSCGTKVCGYDECGVP
                     CGELDGKCPSPLNCNYTDGTCGEYCYPNCTGKACGDDGCGKTCPPGCPDGIECHPDGY
                     CVTEGQCQKSSTELKCEADENSGSGSTAWNIGTTSMALFNYSAACGSTYNPGPEKAFK
                     FVVGPNETGVLEVSISQTLPLLATYLNVYLIEDTGSGCTAASCVAAGSPQGSATKTFQ
                     YEVPASPTGHTYYLVADASISNDGFFSITIKKCTWNGFDDEATETADQ"
     gene            complement(1068..3089)
                     /gene="tkt"
                     /locus_tag="PKH54_09120"
     CDS             complement(1068..3089)
                     /gene="tkt"
                     /locus_tag="PKH54_09120"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOD00097.1"
                     /translation="MKPSDISPELRSRCINTIKMLSVDAIEAAKSGHPGAPMGAADIA
                     FVLWTGFLRFDPKDPAWKDRDRFILSIGHASMLQYSLMHLTGYDLKLDEIRQFRQWGS
                     RAAGHPEYGFLDGVEVTTGPLGQGFAHGVGMALASRMLAARVNTADGGPVGSRVFVLC
                     GDGDMMEGVTYEAASLAGHLGLGNLVCLYDSNRITIEGSTDLAFGEDVAGRFAAQGWD
                     VRTVDGHDHQQIHDAIDAAVGVVDKPSLIICNTFIGRGAATMEGSHETHGAPLGAAEV
                     KATKLALGWPLEPTFHVPADVAEFFSGVGRAGTELHRQWERDFARFVDSNPDRQAAWN
                     GCFDPASPDASVIDRILDSAMSAAGKATRVVFGASLQEAFKSVPNLVGGSADLGPSNN
                     TVIKGGGDVRRGDYSGANLHFGIREHAMGAITNGIALHGAFRPFCATFLIFSDYLKPA
                     ARLAALMRVPSTFVFSHDSFRVGEDGPTHQPVEQLAMLRAIPGFTVLRPADPVEMAGC
                     WSMILERDRGPIAIATTRQNVPAFPRPEAVGPECVRLGGYVLRDCDGTPDVVMLASGS
                     EVQICFAAIPSIEAAGLKVRLVSVPSVTLFMQQPAEYRESILGRSAMLVGVEAGATSC
                     WMPLIQGRGFAVGLDHFGSSAPAGILEQKFGFTPECVATLVVQNRSGRM"
     gene            complement(3157..4044)
                     /gene="panC"
                     /locus_tag="PKH54_09125"
     CDS             complement(3157..4044)
                     /gene="panC"
                     /locus_tag="PKH54_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOD00098.1"
                     /translation="MDAGDITVIRQAAGMQALSRGWHSEGLAVGLVPTMGALHKGHLD
                     LVVEARRQCDRVVVSIFVNPTQFGPSEDFSRYPRDLEGDLAKLADIGVDAVFFPDPGE
                     MYPSGFQTRVELTRLPAHLCGLTRLNHFAGVAQVVLKLFNICKPDVAVFGQKDFQQVR
                     VIEQMVADLNLDIRIARHPIVREPDGLAMSSRNVYLTSEQRQAATVINRTLVDSEAWI
                     REGEHQPAAIVKRAGQQIREAGGEVEYFNVCDPVTLDDLNRIGSESTQVLLVTAVRFG
                     ATRLIDNRLVDCVWAPQPM"
     gene            complement(4044..>4230)
                     /locus_tag="PKH54_09130"
     CDS             complement(4044..>4230)
                     /locus_tag="PKH54_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00099.1"
                     /translation="FLKKYADLASVIRGAVDTFGDEVRGGAFPGPQHSFSGEGLYSSA
                     TAGSSGAVDARKGGGES"
BASE COUNT          920 a         1358 c         1220 g          732 t
ORIGIN      
        1 aaccggcgaa tgcaagccgg gatgcagcgg ggacaaaccg atatgcaacc ttgaaacccg
       61 ccagtgcgaa ccgcagaact gcaaagggtc ttgcggtacg aaggtgtgtg gttacgacga
      121 gtgcggcgtc ccatgtggcg aactggacgg taagtgccca agcccgctca actgcaatta
      181 caccgatggc acctgcggcg aatactgtta tcccaactgc accggcaagg cgtgcggcga
      241 cgacggctgc ggaaagacct gcccgccggg ctgcccggac ggaatcgaat gccacccgga
      301 cggctactgt gtgaccgaag gccagtgcca gaagtcaagc accgagctaa agtgcgaggc
      361 cgatgaaaac agcggatcgg gcagcacggc ctggaatatc ggcaccacca gcatggccct
      421 tttcaactat tcggccgcat gtggatcgac atacaacccg ggcccggaaa aggcgttcaa
      481 gttcgttgtc ggaccaaatg aaacgggggt gctcgaggtc agcatctccc agacactgcc
      541 actgcttgcg acgtacctga acgtatatct gatcgaggac accggctccg gttgcaccgc
      601 ggcaagctgc gtggcagccg gttcaccgca gggaagcgca accaagacat tccagtacga
      661 ggttccggcg tcacccaccg gacacaccta ttacctggtc gcggacgcaa gcatcagcaa
      721 tgacgggttc ttcagcatca ccatcaagaa atgcacatgg aacggtttcg acgacgaagc
      781 gaccgaaacc gcagaccagt gaaattccgt gaatccatga caagctgtgt gatggagaga
      841 gagaatattc attcaggcgg gcgaattttc attcgcccct acagatcgcg gggcggatcg
      901 ggccggaccg taagtgtccg gtgaggcctc tcgaaccgga cacttcatgg ccggccacag
      961 gcacgactgc gtcattcgtc gagcggacgg ttgttcccgg gacgggcgaa ttttcattcg
     1021 cccttacatg tacgggcgga gtaccgcacc tgccttgtac gggcggatta catccgcccg
     1081 ctgcggttct gcacgacaag agtggcaacg cattccgggg tgaaaccgaa cttctgctca
     1141 aggattcccg cgggcgccga gctgccgaaa tgatcgagac cgactgcgaa tccgcgtccc
     1201 tggatcagcg gcatccagca cgacgtcgca cccgcctcga caccgacaag catggcggac
     1261 cgccccagaa tcgactccct gtactcggca ggctgctgca tgaacaaggt taccgacggc
     1321 acgctgacaa gcctgacctt caacccggct gcctcgattg atggaatcgc cgcgaagcag
     1381 atctgaacct cggaaccgct ggccagcatg accacgtccg gggtaccgtc gcaatcccga
     1441 agcacatacc cgcccagacg cacgcattcc ggcccgaccg cttcgggtcg cgggaacgcc
     1501 ggcacgttct gccttgtggt cgcaatggcg atcggtccgc gatcccgttc caggatcatc
     1561 gaccagcacc cggccatttc aaccggatcc gccggacgca gaaccgtgaa tcccggaatc
     1621 gcccgcagca ttgcaagttg ctcgaccggc tggtgggtcg gaccgtcctc gccgacccgg
     1681 aagctgtcgt gcgaaaatac gaaggtcgat ggcacgcgca tcaacgctgc aagcctggcg
     1741 gcaggcttca gatagtccga gaagataagg aacgtggcgc agaacggacg gaaggcgccg
     1801 tgcagcgcga ttccattggt gatcgcgccc attgcgtgtt cgcgaatgcc gaaatgcagg
     1861 ttggcaccgg aataatcgcc tcgtcgaaca tcaccaccgc ccttgatcac ggtattgttg
     1921 gacggcccaa ggtcggcaga tccaccgaca aggtttggaa cggatttgaa tgcctcctgc
     1981 agggaagccc cgaataccac gcgtgtcgcc ttgccggcgg cggacatggc ggaatccagg
     2041 atgcgatcga tgaccgacgc gtccggactg gccggatcga aacatccgtt ccacgcggcc
     2101 tgcctgtccg ggttggaatc cacgaaacgc gcgaagtccc gctcccactg gcggtgaagc
     2161 tcggtgccgg cacgtccgac cccggaaaag aactccgcca cgtcggcagg aacatggaat
     2221 gtcggttcaa gcggccagcc cagggccagc ttcgtcgcct tgacttcagc ggcgccaagc
     2281 ggcgcgccgt gggtttcatg cgaaccctcc atcgttgccg cgccgcgtcc gatgaatgtg
     2341 ttgcagatga taagactcgg cttatcgaca acgccgaccg cggcgtcgat cgcatcgtgt
     2401 atctgctggt gatcatggcc gtcaaccgta cggacgtccc agccctgggc cgcgaaacgt
     2461 ccagccacgt cctcgccaaa ggcgagatcg gtcgaaccct cgatcgtgat gcggttcgag
     2521 tcgtataggc agaccaggtt gccaagcccc agatgtccgg ccagcgacgc ggcctcgtac
     2581 gtgacacctt ccatcatgtc cccgtcgccg cacagcacga aaaccctgga tccgaccgga
     2641 ccgccgtcgg ccgtattgac gcgcgcggcc agcatccttg aagccagcgc catgccgaca
     2701 ccgtgcgcga acccctgccc aagcggccct gtcgtgacct cgacaccgtc caggaagccg
     2761 tattccggat gaccggcagc gcggctgccc cattgcctga actgacggat ttcatccagc
     2821 ttcaggtcgt aaccggtcag gtgcatcagg gagtactgaa gcatcgaggc atgcccgatg
     2881 gacaggatga agcggtcacg atccttccat gcgggatcct tcgggtcaaa gcgaaggaaa
     2941 ccggtccaaa gtacaaaggc gatgtcggcc gcgcccatcg gcgcgccggg atgacccgac
     3001 ttcgccgcct cgatcgcatc gaccgaaagc atcttgatgg tgttgatgca cctggaacgc
     3061 aattccgggg aaatgtccga tggtttcatt tgttgacctc tcatgcctcg ttcacatggg
     3121 cgggcgaatt ttcattcgcc cctgcggatg cctcgttcac atgggctggg gcgcccagac
     3181 gcaatccacc agcctgttgt caatcaaccg cgtcgctccg aacctgacgg cggtcaccag
     3241 cagcacctgc gtggactccg acccgattcg attcagatcg tcaagcgtga cgggatcaca
     3301 cacattgaaa tactcgactt cgccgccggc ttcgcgaatc tgctgccccg cccttttcac
     3361 aatcgccgcc ggctgatgct ccccctcacg aatccatgcc tcggaatcga ccagggtccg
     3421 gttgatgacg gtggcagcct gcctctgttc cgacgtcaga tagacgttcc tcgaagacat
     3481 ggccaacccg tccggctcac gcacgatggg atgccgcgcg atccggatat ccaggttcaa
     3541 atccgcgacc atctgctcga tcaccctgac ctgctggaag tccttctggc cgaagaccgc
     3601 gacatcaggc ttgcagatat taaaaagctt caaaacgacc tgggcgacac cggcgaagtg
     3661 gttcaacctt gtaaggccgc acaggtgcgc gggaagccgg gtcaattcca ccctcgtctg
     3721 aaatccggaa ggatacattt cgccgggatc cggaaagaag acggcatcca cgccaatatc
     3781 ggcaagcttt gccagatctc cttccaggtc gcgcggatat cttgaaaagt cttctgacgg
     3841 accaaactga gtcggattga cgaaaatgct gacaacgacc ctgtcgcatt gccttcgggc
     3901 ttcaacaaca agatcaagat gccctttatg cagggccccc atcgtcggaa caagcccgac
     3961 ggcaagccct tcggaatgcc agccgcggga caaggcctgc atgcccgcgg cctgcctgat
     4021 taccgtgata tcacccgcgt ccatcaggat tcaccgccac ccttccgggc atccaccgcg
     4081 ccggacgaac cggcggtggc agacgagtaa aggccttcgc ccgaaaagga atgctgcggc
     4141 ccgggaaatg ccccgccacg cacttcatcg ccgaaggtat cgaccgcgcc acggattacc
     4201 gacgccaaat cagcgtattt cttcaggaac
//
LOCUS       DAOPPA010000283         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22816_length_4216_cov_4.029801, whole genome shotgun sequence.
ACCESSION   DAOPPA010000283 DAOPPA010000000
VERSION     DAOPPA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22816_length_4216_cov_4.029801"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKH54_09135"
     CDS             <1..912
                     /locus_tag="PKH54_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD00100.1"
                     /translation="GCFRHVIGLTHRLTPEVASAATAVLGRSASGSAAAIRSVHQGGR
                     PVILCSARHARDEAGWIAGRITTMLGGTDMLDSAASGVLTGLGDIAILGRTHNALLPV
                     AKALDAAGLPYETASDAPLWHMDWVRVAMRAIREADPMNRLDSVVSRALAGSGIEPPA
                     RQVRILLDLAGATECGPAFDRLVTLCQVDAFGLDPERIHLLTMHASKGLEFDNVFVVG
                     LNHGMVPLATGGDFDEDEERRLLFVAMTRARRNLFLSWSSAGRQGLPVRSRFLDGLGD
                     LVVQVRAESVVRHRALQQDLFGGGRDA"
     gene            905..1576
                     /locus_tag="PKH54_09140"
     CDS             905..1576
                     /locus_tag="PKH54_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00101.1"
                     /translation="MHESAKIFLGLAAVMLLVSADAVSASDSAMESALVTIERLSGRV
                     PGMDIGGRVSIEILVGKRRLFQTDWRSPGDAEFGNRITFKTAKSMPIRFIVSNWIPKR
                     APGEHVKSPVASGARPPTFREGPAMQDDVLATGFDGLIGDYKVDGGVPAADQASQRIS
                     EGKSRDSGASSHDERPNGRQSVLCEAQIDWPPANGIHRIDCGGASLEISTVMLKRAKS
                     RPEAR"
     gene            complement(1542..2576)
                     /locus_tag="PKH54_09145"
     CDS             complement(1542..2576)
                     /locus_tag="PKH54_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00102.1"
                     /translation="MSRKTTMETLKGGMLLMILLLLLLLIAGGIVRWRYGARDRGPAV
                     KVTVNVQGDARQPNTDAGAGIDDQWAGGVFPVPKGTVETSRAIDATVPGSHSVDTDID
                     GAVAGIQHRRIFCRTVARESDPAAGVQICSVLFDDALDALVVFGRMSPGPSSLRMPGL
                     TSGSVDPDGFHLVKGTRYFRIRRGSQTEDARAVAMAAVSMIASSAPAASDTGFERLLP
                     TRGRVEGSWKIRSGTDIGLDFLGTVLTCDYSAPRFEMSLFMKKAGTTESAGKLLEQAI
                     QSAVRQGAVEQHVPATESGLKVFQVGATLEVYGIAGTTVFGIRRASGITQAIELAREI
                     ILLQDGTSPF"
     gene            complement(2656..4119)
                     /locus_tag="PKH54_09150"
     CDS             complement(2656..4119)
                     /locus_tag="PKH54_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00103.1"
                     /translation="MRLFPVTAFAGVMMLLVACGNGSGDQDVVGGDNNVTRPDNGTIN
                     FDVVNPDIPTINKECQTNLECQEKNGGEWWACQKCETEWKCVQPAAMGEPGWGCVENV
                     NCGSEAYCHSCENVCKPLLEACQTCENDEQCAGQMSHCVDKVNYQGVESELPGKICAP
                     WCPLSTKVCAVPGAPQGSYVCADIGDASNGVCVPATMNCDSTIEKCESDEDCEQEGFK
                     CYTDLGICGCRDALSCELGQACHPVTHQCMMGCADDNECGNGTVCSQGLCVPPCTGTL
                     EKQNVQGCTGTVPDGYMWDCDETGHCFVPGMCFQPSDCKEPATYCDAETHKCLSGCMF
                     DFDCKTFSQICDTTTNPGTCIRRPCKGNYSCSCGEVCELTQGECQTAEGNYCAVCDQQ
                     ADDPCGNEDTLCIGFQDKDGNDMGSYCMPPCSSDPDNLCPQGWQCQEVKNQDGSPAGK
                     VCVRHCYQKVAGGCAMGDAPDPQPADGTTSETATADM"
BASE COUNT          753 a         1282 c         1314 g          867 t
ORIGIN      
        1 ggttgtttca ggcatgtcat aggtcttacg cacaggctta ccccggaagt cgcgtcggcg
       61 gccactgcgg ttctcggacg atccgcatcg gggtcggcgg ccgcgatccg ttcggtgcat
      121 cagggcggta ggccggtcat tctgtgctcc gcccgacatg cgcgggacga ggccggctgg
      181 attgcaggca ggatcaccac gatgcttggc ggaaccgaca tgcttgattc ggcggccagc
      241 ggtgtgttga ccggccttgg ggacatcgcg atcctcgggc gcacgcacaa cgccctgctg
      301 cccgtcgcaa aggccctgga cgccgccggc ctgccttatg agaccgcgtc cgatgcgccc
      361 ctgtggcaca tggactgggt tcgtgtcgcc atgcgggcaa tccgggaggc cgatccgatg
      421 aaccgcctgg attccgtggt ttcccgcgcg ctggccggat cgggcatcga gccgccggcc
      481 cgccaggtca ggattcttct cgacctggcc ggggcgaccg aatgcggccc ggctttcgat
      541 cggcttgtga ctctttgcca ggttgatgcg ttcggtcttg atccggaaag gattcacctt
      601 ctgaccatgc acgcgtcgaa ggggctggaa ttcgataatg ttttcgtggt cggcttgaat
      661 cacggcatgg ttccactggc aacaggcggc gatttcgatg aagatgagga gcgcaggctt
      721 ctttttgtgg cgatgacacg ggcacgaagg aacctttttc tttcgtggtc ctcggccggg
      781 cgtcaggggc ttccggttcg aagccgcttc ctggacggcc tgggtgattt ggtggttcag
      841 gttcgggccg agtcggttgt caggcacagg gcattgcagc aggatctttt tggcggcggc
      901 agggatgcat gaatcggcga aaatcttcct ggggctggcg gcagtgatgc tcctggtatc
      961 ggccgacgcc gtttctgctt cggattcggc gatggaatcg gccctcgtta cgattgaaag
     1021 gctgtctggc cgcgtgccgg ggatggatat cggggggcgc gtctcgatcg agatcctggt
     1081 tggaaagcgg cgccttttcc agaccgactg gagatcgccc ggggacgccg aattcggtaa
     1141 ccggatcacg ttcaagaccg cgaaatcaat gccaatcagg ttcattgtca gtaattggat
     1201 accgaagcgc gcgccgggcg agcacgtcaa gagccccgtg gcatccggtg cccgcccgcc
     1261 cactttcagg gaaggccccg cgatgcagga tgacgtcctg gcgaccggtt tcgacgggtt
     1321 gatcggggat tacaaggtcg atggaggggt tccggcggcc gatcaggctt cccagcgcat
     1381 ttcggaaggg aagtcccgcg attccggggc cagctcacat gacgagcggc cgaatggccg
     1441 tcagagcgtc ctgtgcgagg cgcagattga ctggccgcca gcgaacggaa ttcatcgaat
     1501 cgactgcggt ggagccagcc tggaaatttc aaccgtgatg ctcaaaaggg cgaagtcccg
     1561 tcctgaagca agatgatttc ccgtgccagt tcgatggcct gggtaattcc ggaagcccgc
     1621 ctgatcccga agaccgtcgt gccggcaatg ccatacactt cgagcgtggc gccgacctgg
     1681 aagaccttca ggccgctttc ggtcgccgga acgtgctgtt caaccgcgcc ctgcctgacg
     1741 gccgactgaa tggcctgttc cagcagcttt ccggcagatt cggtcgtccc cgccttcttc
     1801 atgaacaggc tcatttcgaa ccgtggcgcg ctgtaatcgc aggtcagaac cgttccaagg
     1861 aaatcaagac cgatgtctgt tcccgaacgg atcttccatg aaccctcgac tcggccacgg
     1921 gttggcagaa gtcgttcaaa cccggtatcc gacgccgccg gcgcggatga ggcgatcatc
     1981 gagactgccg ccattgcgac agcgcgggca tcttcggtct ggctgcctcg ccggattctg
     2041 aagtaccggg tgcccttgac gaggtgaaaa ccgtccggat cgaccgaacc cgaagtcagg
     2101 cctggcatgc gcaacgaaga tggcccgggc gacatgcgtc cgaacacgac aagcgcgtca
     2161 agagcgtcat cgaaaagtac cgaacagatc tgcacgcccg ccgcgggatc cgattccctg
     2221 gcgacggttc ggcagaagat tcttcgatgc tggattcccg ccaccgcccc atcgatatcc
     2281 gtgtcgacag agtgcgagcc gggaaccgtc gcgtcgattg cccgggatgt ctcaacggta
     2341 cccttcggca cgggaaacac tcccccggcc cactgatcgt cgatcccggc acctgcgtcc
     2401 gtgttgggct ggcgtgcgtc cccctggacg ttgacggtga ccttgactgc cgggccgcga
     2461 tcgcgcgcgc catatcgcca tcgtacgatt cccccggcga tcagcagaag gagcagaagc
     2521 aggatcatca ggagcatccc gcccttaagc gtttccatgg tggtctttcg ggacatgcag
     2581 cctccgcggt tcgcggcatc gttgccaagc tgtcgcgcaa ttattgcagg aaatcaggaa
     2641 aaggtgtcgt ggtgactaca tgtcggcggt cgcggtctcg gaagtcgtgc cgtcggccgg
     2701 ctggggatcc ggtgcgtcgc ccatggcgca tccgccggcc accttctggt agcagtggcg
     2761 gacacagacc ttgcccgctg gcgaaccatc ctggtttttg acttcctggc actgccagcc
     2821 ctgggggcac aggttgtccg ggtcgcttga acaaggtggc atgcagtatg aacccatgtc
     2881 attgccgtcc ttgtcctgga agccgatgca cagggtgtcc tcgttgccgc agggatcgtc
     2941 cgcctgctgg tcgcaaacgg cgcagtagtt gccctcggcc gtctggcatt cgccctgggt
     3001 cagttcgcag acctcgccgc acgaacacga gtaattaccc ttgcacggac gcctgatgca
     3061 cgtcccgggg tttgtcgtcg tgtcgcagat ctggctgaaa gtcttgcagt caaagtcgaa
     3121 catgcatccg gagaggcatt tgtgggtttc cgcgtcgcag tacgtggccg gttccttgca
     3181 gtccgaaggc tggaagcaca tgcccggtac gaagcagtgt ccggtttcgt cgcagtccca
     3241 catgtaaccg tcgggcacgg tgccggtgca gccctggaca ttctgctttt ccagagtgcc
     3301 ggtgcagggc ggaacgcaca atccctggga gcatactgtg ccatttccgc attcgttgtc
     3361 atcggcgcag cccatcatgc actggtgggt gaccgggtgg cacgcctggc cgagttcaca
     3421 ggacagggca tcccggcagc cgcagatgcc aagatcggtg taacacttga agccttcctg
     3481 ttcgcagtct tcgtcggatt cgcacttctc gatcgtcgag tcgcagttca tcgtggccgg
     3541 gacgcaaacg ccgtttgacg cgtcgccgat gtccgcgcag acatatgaac cctgcggcgc
     3601 gcccggaacg gcacaaacct tcgtcgaaag cgggcaccac ggggcgcaga tcttgccggg
     3661 aagttcggat tcaacaccct gatagttgac cttgtcgacg cagtgtgaca tctgtccggc
     3721 gcactgctca tcgttttcgc acgtctgaca tgcttcaagc agcggcttgc atacgttttc
     3781 gcacgaatgg cagtaagcct cggaaccgca gttcacgttc tcgacacagc cccagccggg
     3841 ttcgcccatg gccgctggct ggacgcattt ccattccgtc tcgcacttct gacaggccca
     3901 ccattcgccg ccgttctttt cctggcattc caggttcgtc tggcattcct tgttgatggt
     3961 gggaatatcg gggttgacga cgtcgaaatt gatcgtgcca ttgtccggcc tggtgacgtt
     4021 gttgtcgccg ccaaccacgt cctgatcacc ggaaccgttg ccgcaggcga ccagaagcat
     4081 catcacgccc gcaaaggccg taaccgggaa taatctcatc tgaaacctcc agggatctcc
     4141 aggcgatgtc cgccgtcttc caatacaaaa acgcacccgc cgaattttac acactgcccg
     4201 gtttcaaggc cagtag
//
LOCUS       DAOPPA010000284         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22827_length_4215_cov_3.828846, whole genome shotgun sequence.
ACCESSION   DAOPPA010000284 DAOPPA010000000
VERSION     DAOPPA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22827_length_4215_cov_3.828846"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKH54_09155"
     CDS             complement(<1..1212)
                     /locus_tag="PKH54_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00104.1"
                     /translation="MNRKISLVAMATVALVAVGVLGCKPAAAPEPKEKVEFSMHIMSQ
                     CPFGAQVQKGIAPVLKTLGNTVDFKMEFIGDEPQPGNLTSMHGANEVNGNKYQICAKK
                     YAPANYMDVVVCMANDMRNIPQNFEKCATEAGIDPAPIKACAEGQEGTDLLSASFKAS
                     KEAGARGSPTMFLNGEKYQGGRTETDFMRSICNAFKAKKPSVCANIPEPKKIAVTVLS
                     DRRCTKCFPDRILGQLKNMFPGLEPKTLDYSDSEGKALYNSLKDQGVKLLPAFLFDPV
                     VVDDAGFSQIKRFVVDAGQYKLLQVGAKFDPTAEICDNGVDDDGDGRVDCRDSDCEGS
                     SECRSEIARRLDLFVMSQCPFGVKALNALPEFIDAFKYDGISVNINYIADEMPDGSFK
                     ALHGQPEVDENI"
     gene            complement(1325..2239)
                     /locus_tag="PKH54_09160"
     CDS             complement(1325..2239)
                     /locus_tag="PKH54_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOD00105.1"
                     /translation="MAGKGRKRFGRAAARLGRGLLQEVRVGLSRLIPAGRLFYGSRGF
                     DHAAAMSFYFILSLAPFVVLLVSATGFMAANMGQDPARLDEMIEKFAVYVRTWIPVES
                     DTVRDIVNYLISRKTSFGLAGTAVLVLGASAIFGAIENATADIFRNGVRRRYIVSRLI
                     FTIGIATAGLLAFMLYNAVTLVQSFVDARIGASVLKTLLDKSSFTVLFEWLPVPAGFL
                     IILYGTGIARPKFRDALRGAALYFVLWTVVREAYGHYVTEVANYSMLYGSLATPILLI
                     LWLFYSSLILVYCLCFVAVPGLPESGRE"
     gene            complement(2250..3557)
                     /locus_tag="PKH54_09165"
     CDS             complement(2250..3557)
                     /locus_tag="PKH54_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00106.1"
                     /translation="MKYHVLPVLALLMSVMMPARAHAEPGDVPAVGDLFDSIHVVIGP
                     SRQSLQSIAVQQVACEGADKSCTQAGGTVSRDLEISGFFKVLDPRSFLADPKAETLVA
                     TSWADWMNVGASWLVKYSVKKAGERVDVEFRLYDVNEKRARQVSAQSFPGISPTKVRG
                     AAHRFVNAVIKEITGQPGIFGSRIVVSLKSDAATRDIVTMEMDGSGRGVAVKNGSANM
                     FPRWSKGGKLLYTSFKSGLPQLYLGDRQLTDGVHEYRGAEMSPDGKSIAASVDMDGQS
                     DIVLIDSDTGKLVRKLTDTPWDEVSPTWSNGGNLIAYVSSEAGRPQIHIMNSDGSGQR
                     RLTMAGQYNTSPRFGPGNRVVFSGMDMFRSDLFVVDLEGNISRLTQEQGNNKDAAWSP
                     DGRYLVFLSDRTGDWQVWIMTEDGRYQFPISPDRGRFGTPDWR"
     gene            complement(3688..>4215)
                     /locus_tag="PKH54_09170"
     CDS             complement(3688..>4215)
                     /locus_tag="PKH54_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00107.1"
                     /translation="EVCRSAASIEIRKYNGDGTCAVALDEQGNCPILMETVGEVKGDF
                     SLVRNSPMCPTFDVKNGERPENAIQFTYPLGYTDKLEITYRGGVDFCLNRCTRSTSSD
                     AKDQEYCREICGGNTDDCLKGCLIDHDNCAYVCNVVVDDCVNSCNGFTSQCTGGTCSF
                     NLTGAECVESCKAVE"
BASE COUNT          905 a         1292 c         1184 g          834 t
ORIGIN      
        1 tatattttca tcgacttccg gctgaccatg cagcgccttg aagctgccgt cgggcatttc
       61 gtcggcaata taattgatgt tgacggaaat tccgtcgtac ttgaacgcat cgatgaattc
      121 cggaagtgcg ttaagcgcct tcacgccgaa cgggcactgg gacatcacga acagatccag
      181 cctgcgcgcg atctctgatc gacactcgga agaaccctca cagtcggagt ccctgcagtc
      241 aacccttccg tcaccgtcgt cgtccacgcc gttgtcgcag atttcggcgg tcggatcgaa
      301 cttcgcgcca acctgcagca gcttgtactg accggcatcg acgacgaagc gcttaatctg
      361 gctgaaaccg gcgtcatcga ccacgacagg atcaaacagg aatgccggca gcagcttcac
      421 gccctgatcc ttcaggctgt tgtacagcgc cttgccttcc gaatccgaat aatcgagggt
      481 cttcggttca agtccgggga acatgttctt caactggcca aggatcctgt cggggaagca
      541 cttcgtgcag cgcctgtcgg acaggacggt gacggcgatc ttcttcggct ccgggatgtt
      601 cgcgcagacc gacggctttt ttgccttgaa cgcgttgcaa atcgaacgca tgaagtcggt
      661 ctcggtgcgg ccaccctggt acttctcgcc gtttaagaac atcgtcgggg atccgcgggc
      721 gccagcctcc tttgatgcct tgaaggaagc tgacagcagg tcggtgcctt cctgaccttc
      781 ggcgcacgcc ttgatcgggg ccgggtcaat cccggcctcg gtcgcgcact tttcgaaatt
      841 ctgcggaatg ttgcgcatat cgttggccat gcagacgacc acgtccatgt agttggccgg
      901 cgcgtacttc ttcgcgcaga tctggtactt gttgccgttg acttcgttgg cgccgtgcat
      961 cgacgtcagg ttaccgggct ggggctcgtc accgatgaac tccatcttga aatcaaccgt
     1021 gtttccgagg gtcttaagca cgggggcgat acccttctgg acctgggctc caaacgggca
     1081 ctgcgacatg atgtgcatcg agaactcgac cttttccttg ggctccgggg cggccgcggg
     1141 cttgcaacca agaacgccca ccgcaaccag ggccacggtt gccatcgcaa ccagactgat
     1201 cttccgattc atcgtcccac cataattaaa aagccaattc aaatcccgaa tatatcggtc
     1261 cgcgtgatct taccagcaac cacgcagaat gcaacaacgt cagccaacaa tggtccggga
     1321 atttttattc ccgcccggat tccggcagtc ctggaacggc aacaaaacaa aggcaataga
     1381 cgaggatcag cgacgaatag aacagccaca ggataagcag gatcggcgtg gcaagggaac
     1441 cgtacagcat gctgtagttg gccacctcgg tcacgtaatg cccgtacgcc tccctgacga
     1501 cggtccagag aacgaaatag agcgcggcac cgcgcagcgc atcgcggaac ttcgggcggg
     1561 caatccccgt tccatacagg ataatcagga atccggccgg gacgggaagc cattcaaaaa
     1621 gaaccgtgaa ggatgacttg tccaaaagag tcttcagaac cgaggcgcca atcctggcat
     1681 cgacgaacga ctgtacgagc gttaccgcgt tgtaaagcat gaacgccaga aggcccgcgg
     1741 tcgcaattcc gatggtgaat atcaggcgcg acacgatata gcgccgcctt acgccgttgc
     1801 ggaagatgtc cgccgtggcg ttctcgatgg ccccgaatat cgcggatgcg ccgagcacca
     1861 ggacggccgt gccggcaagc ccgaagctgg tcttgcgtga aatcaggtaa ttgacgatgt
     1921 ccctgaccgt gtccgactcc accgggatcc acgtcctgac gtacacggca aacttttcga
     1981 tcatctcgtc caggcgggcg ggatcctgtc ccatgttggc ggccatgaaa ccggtcgcgg
     2041 aaaccagaag gacgacaaac ggtgccaggg aaaggatgaa atagaacgac atggcggcgg
     2101 cgtggtcgaa accccttgaa ccgtaaaaga ggcggccggc cggtatcagc cttgaaagac
     2161 cgaccctaac ctcctgcaga aggcccctgc cgagccgcgc ggctgcgcga ccaaaccgct
     2221 ttcgcccttt tccagccaaa cgcctttcct caacgccagt ccggggttcc gaaccgaccg
     2281 cgatccggcg atatcgggaa ttgatatctg ccgtcctcgg tcattatcca tacctgccag
     2341 tccccggttc tgtctgaaag gaacaccagg taccttccgt ccggcgacca cgccgcgtcc
     2401 ttgttgttgc cctgttcctg ggtaagcctt gaaatgtttc cttcaaggtc aaccacgaag
     2461 agatctgacc ggaacatgtc cattcccgag aaaacgaccc ggtttcccgg gccgaaacgc
     2521 ggcgaagtgt tgtactggcc ggccatcgtc aaccggcgct ggccggatcc gtccgaattc
     2581 atgatatgga tctggggcct gccggcttca gacgagacat acgcgatcag gttgccgcca
     2641 ttcgaccagg tcgggctgac ctcgtcccac ggggtgtcgg tcagcttcct taccagcttt
     2701 ccggtgtctg aatcgataag aacgatatcc gactgcccgt ccatgtcaac cgaagcggca
     2761 atcgacttcc cgtcgggcga catctcggcg ccccggtatt catgaacacc gtcggtcaac
     2821 tgccgatcac ccagataaag ctgtggaagc ccggacttga aggacgtgta aagcagtttg
     2881 ccgccctttg accaccttgg aaacatgttg gcggaaccgt tcttcacggc cacgccgcgc
     2941 ccgctgccat ccatctccat cgtcacgatg tcgcgggtcg cagcatccga cttcagggaa
     3001 acgacaatcc tggatccaaa gatgccgggc tggccggtga tttccttgat taccgcattg
     3061 acgaacctgt gggcggcacc gcgaaccttt gtcggggaga tgcccggaaa cgactgtgcc
     3121 gagacctggc gcgcgcgctt ttcgttgacg tcgtacagac gaaactcaac atcaacgcgc
     3181 tcaccggcct ttttgaccga atacttcacg agccatgagg ccccgacatt catccagtcc
     3241 gcccagcttg tggcgacaag cgtctcggcc tttggatccg caaggaagga tcgtggatcc
     3301 agcaccttga agaaaccgga tatctcgaga tccctgctga cggtgccgcc ggcctgggtg
     3361 caggacttat ccgcgccctc gcaggcgacc tgctgtaccg caatcgactg aagggactgc
     3421 ctggacggac cgatgaccac gtggatcgaa tcaaacagat ccccgaccgc gggaacatcg
     3481 ccgggctccg catgcgcccg ggcgggcatc atcactgaca tgagcagggc gagaaccggc
     3541 aagacatggt atttcattgc attccccgaa aatcggcccg acgtttcccg gccgggcggt
     3601 cacgccattg tacaacgaca aggcggctat ccgccaaacg cgggtattgc ccgaatgatt
     3661 cccgggaaag gggtggaaga tgcgggacta ctcgacggcc ttgcaggatt cgacgcactc
     3721 ggccccggtc aggttgaatg agcaggttcc gcccgtgcac tgcgacgtaa agccgttgca
     3781 cgaattgacg cagtcatcga caacgacgtt gcacacatag gcgcagttgt cgtgatcgat
     3841 aaggcagccc ttcaggcagt cgtcagtatt accgccgcag atttcccggc agtactcctg
     3901 atccttggcg tcggaagatg tcgatctggt gcaacggttc aggcagaaat caacgccgcc
     3961 gcggtaagtg atttccagct tgtccgtata tccaagcggg tatgtgaact ggatggcgtt
     4021 ctcgggacgc tcgccgttct tcacgtcgaa cgtcgggcac atcggagaat tgcgaaccag
     4081 gctgaagtcg cccttaacct cgccgaccgt ctccatcagg atcgggcagt tgccctgttc
     4141 gtccagcgcg accgcgcacg tcccgtcacc gttgtacttt cggatctcga tggacgcggc
     4201 cgaacggcac acctc
//
LOCUS       DAOPPA010000285         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22891_length_4206_cov_3.803421, whole genome shotgun sequence.
ACCESSION   DAOPPA010000285 DAOPPA010000000
VERSION     DAOPPA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22891_length_4206_cov_3.803421"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH54_09175"
     CDS             complement(<1..226)
                     /locus_tag="PKH54_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD00108.1"
                     /translation="MSDWIDVSVPLSTGITVWPGDPAVEIVRFSRMSDGAPCNVSSIS
                     TSLHAGTHVDAPLHYVDRGIDIAHLPIDHLC"
     gene            complement(251..2428)
                     /locus_tag="PKH54_09180"
     CDS             complement(251..2428)
                     /locus_tag="PKH54_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOD00109.1"
                     /translation="MGVSEQMNDFCARISSVTGLARPGVMAVLELGESGATVPFIARY
                     RKEASGGMDEVQVRLVLKLGQELQELEKRRSAILESLQERELLTDALRQAVMAADSRA
                     RLEDVYLPFKPKRRTRASIAVERGLARLAEMISRQGRDGNPDVEAQAFVNLEKGVEDG
                     QAALAGARDIVAEFVSDNAAVRAMVRRMMLDSGTVDSQAPKRVRESGEPTPYEQYYSF
                     SQSVRAIPSHRFLAIRRGAREEILSMSIGIDASGILEQIHSICGLKSSSPWAGQLRLA
                     CMDAWKRLLFPAMENEVLSTLAEHCEREASEVFAANLRDLLLAAPYGPRAVIGLDPGI
                     RTGCKLAAVNADGTYVSSAVIFPDRNPAEAASVIVAMARKIRATAVAVGNGTYGREAL
                     AFVRQALSDAGLQDVVAVAVSEAGASVYSASDIARAEFPDLDLTVRGAISIARRLQDP
                     LAELVKVEPRSLGVGQYQHDISPALLDQRLDEVVEDCVNSVGVDLNTASAPLLSRVAG
                     IGPSLATSIVAFRQAQGQFTDRRQLLKVPRLGPKAFEQCAGFLRIRGGRNPLDASAVH
                     PERYDLVTAMAADIGVPVAGLVGDPALVGRIDLSRYVRGQPGGVDSVGMPTLVDIAAE
                     LKRPGRDPRSTFEAPAFREDVREIKDIVPGMVLEGQVTNVAAFGAFVDIGVHQDGLVH
                     ISELSERYVKDPLEVVNVGKKVKVRVLAVDPVRRRISLSMKDT"
     gene            2591..3631
                     /gene="prmA"
                     /locus_tag="PKH54_09185"
     CDS             2591..3631
                     /gene="prmA"
                     /locus_tag="PKH54_09185"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOD00110.1"
                     /translation="MMNGPDDAPVKHPAWVEVTVTVPKPARDAVIGWFMDRGSAGVQE
                     DYPGLFPAEGTGPVTSGDPREWAGEAPPNMTESVLLRAWLPPPVSTESAAAALESMLK
                     QLEGQWPALRDAQRQVREIESIDWNAEWKSHWKPVEIGRGFLVCPSWEKPPATDRKVI
                     FLDPGMAFGTGTHFTTASCIEAAEDWFDTVADRSGVSVLDVGTGSGILAIAALKLGAR
                     DAFGIDVDPDAARESIRNADANCVADRYVGTDRPLTGQEGMFQLVFANILAQTLVFLA
                     PRIAATVMPGGCLIASGILREYEDEVTGTFLDLGFTVVSKRHDESWSTLTFRMDGPRE
                     SDCAPRRGNARQ"
     gene            3628..>4206
                     /locus_tag="PKH54_09190"
     CDS             3628..>4206
                     /locus_tag="PKH54_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD00111.1"
                     /translation="MKQNGLLSRILRYFSTDPDLPRIQADDKVIKRMFEWRRLSVFLS
                     ITIGYGIYYVGRLNFSVVKPAMLDEKVLSATQMGIVDSALLFAYAVGKFSNGFIADRA
                     NVRKFMSFGLLAVAIINLILGSTSMFIMFVALWGLNGWFQSIGAPSSIVSLANWYSPR
                     ERGTWYGIWCTSHDIGGALNVIITAVVVEALGW"
BASE COUNT          827 a         1326 c         1273 g          780 t
ORIGIN      
        1 cgcagaggtg gtctatcggc aggtgtgcga tgtcgatgcc gcggtcaaca taatgaagcg
       61 gggcatcgac atgcgtcccg gcgtgcaggc tggtcgaaat cgaggacaca ttgcaaggtg
      121 cgccgtccga catcctggaa aacctgacta tctcgaccgc gggatccccc ggccagaccg
      181 taatgccggt cgaaagggga actgagacat caatccaatc cgacatcctg gactcctgca
      241 gagcacacgg tcaggtatcc ttcatcgaaa gcgatatcct gcggcgaacc ggatcgaccg
      301 caagaaccct gaccttgacc ttcttgccga catttaccac ttccagagga tcttttacat
      361 acctttccga cagttccgaa atgtgaacaa ggccgtcctg gtgcactccg atatcgacga
      421 aggcgccaaa ggcggcgaca ttggtgacct gcccctccag aaccataccc gggacgatat
      481 ccttgatttc ccggacgtcc tcgcgaaatg ccggggcctc gaacgtgctt ctgggatcgc
      541 gcccgggacg cttcaactcg gccgcgatgt caacaagcgt cggcatcccg acggaatcga
      601 ccccgccggg ctgtccgcgg acgtacctgg acagatcaat cctgccaacc agtgccggat
      661 cgccgaccag cccggcaacc ggaacgccga tgtcggcggc catcgccgtg accaggtcgt
      721 acctttcggg atgaacggcc gatgcgtcca gaggatttcg cccgccacgt attctcagga
      781 accccgcgca ctgctcgaaa gccttcggac ccagccgggg aaccttcagg agttgtcggc
      841 gatcggtgaa ctgcccctgg gcctgcctga acgccacaat cgacgtggca agtgacggac
      901 cgatgcccgc tacccgggac agcagaggcg cgcttgcggt gttcagatcc acgccgacgc
      961 tgttgacgca atcctcgacg acctcgtcaa gcctctgatc cagaagggcc ggcgaaatgt
     1021 cgtgctggta ctgcccgacg ccaagcgaac gcggctcgac cttgaccagt tccgccagcg
     1081 gatcctgcag gcggcgcgca atcgaaatcg cgccgcgcac ggtcaggtcc agatcgggga
     1141 actcggccct ggcgatgtcg gacgccgaat aaacgctggc accagcctcg ctgaccgcta
     1201 ccgccaccac gtcctgcaac ccggcgtcgg aaagggcctg gcggacaaac gcaagcgcct
     1261 cgcggccgta cgtcccgttg ccgactgcaa cggcggtcgc gcggatcttt cgggccatgg
     1321 ccacgatcac cgatgcggcc tcggcgggat tgcgatccgg gaatatgacg gcgcttgaca
     1381 cgtaagtacc gtccgcgttc acggcggcca gcttgcaccc ggttcgtatt ccggggtcca
     1441 ggccgattac ggcccttggg ccataaggcg cggcaagcag aaggtcccgc aggtttgccg
     1501 cgaatacctc ggatgcctcg cgttcacaat gttccgcaag cgttgacagc acctcgtttt
     1561 ccatggccgg aaacagcagc cgcttccatg cgtccatgca cgcgagcctt agctgcccgg
     1621 cccacgggct tgacgatttc aggccgcaaa tcgagtgtat ctgttcaagt attccggatg
     1681 cgtcgatgcc gatggacatc gaaagaatct cttcgcgcgc cccgcgtcga attgccagga
     1741 agcggtgcga tggaatggct ctgaccgact ggctgaaact gtaatactgc tcgtacggag
     1801 tcggttcgcc ggattccctg acacgctttg gcgcctggga atcgaccgtt ccggaatcaa
     1861 gcatcatgcg gcggaccatc gcccgaaccg ccgcgttgtc ggaaacgaac tcggcaacga
     1921 tgtcgcgggc gccggcaagg gcggcctgtc cgtcttcgac gcccttttcc agattgacaa
     1981 aggcctgggc ctcaacgtcc ggattgccgt cacgcccctg ccgcgaaatc atttcagcaa
     2041 ggcgggcaag gccgcgttcg acggcaatgg atgccctggt cctgcgtttc ggcttgaacg
     2101 gcagatagac atcctccagc ctggcgcggg aatcggccgc catcaccgcc tggcggagcg
     2161 cgtcggttag gagttccctt tcctgaaggg attccagaat ggccgaccgg cgcttttcca
     2221 gttcctgcag ttcctggccg agcttcagaa ccagcctgac ctgaacctcg tccatgccgc
     2281 cgcttgcttc cttgcggtac ctggctatga acggaacggt ggcgccggac tccccaagtt
     2341 ccagcaccgc catcacgcca gggcgcgcca gcccggtaac cgaggaaatc ctggcgcaga
     2401 agtcattcat ctgttccgaa acacccatcg gcgccaactc ccgtgcttcc gaaatggagc
     2461 gccgatcata agcgggatgg gactgaagtg gaaatccggg aaagtgccgg cgccatttca
     2521 cgcagtggcg gaccggtgta tagtatggcc cgttaaacgg cgacgaacag gccgtcaaca
     2581 aggattccgg atgatgaatg gccccgacga cgcaccggtt aaacaccctg cctgggtgga
     2641 agtcacagtt accgtgccga agccggcacg agacgcggtc ataggctggt tcatggacag
     2701 gggttcggcc ggcgtccagg aagactaccc gggactgttc ccggccgaag gcactggccc
     2761 ggtaaccagc ggggaccccc gcgaatgggc cggcgaggcc ccacccaaca tgacggaatc
     2821 ggtcctgctg cgcgcctggc ttccaccacc ggtttccacc gagtcggcgg cggccgctct
     2881 ggaatcgatg ctgaagcagc ttgagggaca gtggccagcg cttcgcgacg cgcaacgcca
     2941 ggttcgcgaa attgagtcca tcgactggaa cgcggaatgg aagtcgcact ggaagccggt
     3001 tgaaatcggc cgcggattcc tggtctgtcc atcctgggaa aagccccctg ccacggatcg
     3061 caaagtgata ttccttgatc ccgggatggc tttcgggact ggaacccatt tcacgacggc
     3121 aagctgtatc gaggcggccg aggactggtt cgacacggtg gccgaccggt caggcgtgtc
     3181 cgttctcgat gtcggaaccg gttcaggaat ccttgccatc gcggcgctga agcttggcgc
     3241 cagggacgcc ttcggcatcg acgtggatcc ggatgccgca agggaatcga ttcgaaacgc
     3301 ggacgcaaac tgcgtcgcgg acagatatgt cgggaccgac cggcccctta ccgggcagga
     3361 aggcatgttc cagctcgtct tcgccaacat cctggcgcag acgctggttt tcctggcccc
     3421 gaggattgcg gcaaccgtca tgccgggcgg ctgtctgatc gcctcgggga tactgcggga
     3481 atacgaggac gaggtgaccg ggacatttct tgatctcggc ttcacggtcg tatcgaagcg
     3541 acatgatgag tcctggtcaa ccctgacttt tcgcatggac gggccgcggg aatccgattg
     3601 cgccccgcgc agaggtaacg ccagacaatg aaacagaacg ggttgctgtc acgaatcctt
     3661 cgctattttt ccaccgatcc ggatctgcct cgaatccagg cggacgacaa ggtcatcaag
     3721 cgcatgttcg aatggcgcag gctgagcgtc tttctgtcga tcacgatcgg ttacggaatc
     3781 tattacgtcg gcaggctgaa cttttcggtc gtgaagccgg cgatgcttga cgaaaaggta
     3841 ctgtcggcca cccagatggg catcgttgac tcggcactcc tcttcgccta cgcggtgggc
     3901 aagttctcga acggcttcat tgccgacagg gccaacgtca ggaagttcat gtccttcggc
     3961 ctgctcgcgg ttgccatcat caacctgatc cttggttcga ccagcatgtt catcatgttc
     4021 gtggccctgt ggggccttaa cggatggttc cagtcgatcg gggcgccatc cagcatcgtt
     4081 tcgctggcaa actggtattc cccccgcgaa cgcggaacct ggtacggcat ctggtgcacc
     4141 agccacgaca tcggcggcgc gctgaacgtg atcattaccg ccgtcgtggt tgaggccctt
     4201 ggctgg
//
LOCUS       DAOPPA010000286         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22898_length_4205_cov_3.585542, whole genome shotgun sequence.
ACCESSION   DAOPPA010000286 DAOPPA010000000
VERSION     DAOPPA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22898_length_4205_cov_3.585542"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKH54_09195"
     CDS             <1..200
                     /locus_tag="PKH54_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00112.1"
                     /translation="MANLGAFIKFWVTDYLAVGIDYRHYIYPKAPGFDAQNVSKGEWT
                     NGVSSLAEISFGLTYVTPAPK"
     gene            267..2159
                     /locus_tag="PKH54_09200"
     CDS             267..2159
                     /locus_tag="PKH54_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00113.1"
                     /translation="MTRFSFSKLLLAVVITGTVLVSGPAGAITKDEVIVLATQLMVPE
                     ADIIATIEKDRSIFKLEISDILELRKAGVPDAVIKFMMQTPTRFAQPVQQAPAVQQQQ
                     AAAVPAAPVVREKTPEEIEAERRFAAEQAAAREAEMKAAEEARRRAYASGKLRKGLEL
                     ADQGDWVNALQLFQSFLAEENYADDSIEVYNAKYGMAAALTNAGLYQSAARLLVEVLL
                     QGTERPFFQEAFRKLRVLRQRVNYTPHELETLTTFPMVAFDATFQVEFNYVMGQYFYD
                     IGDFERAVTHLNQVGDAGPDAAKARYLVGLVQVRNQMYKSAVESFQSAVNLAEGAFKD
                     SGVRDLAYMALARIAYEAENFDAAIFYYKKVPTDSPRAGTVFFELAWTYLMKADYSRA
                     LGTFHAIRSPYFRDAFLPDLWILEARVYGDLCHFDKAEKALGMFDQEIVVHQIPLRRF
                     MDAQASLENYYDAFLKSVNEPDDKSLPQVLTLPVMSDVEFYNLYRTIKQIDKEISMIQ
                     GNSGALGAFAEDMQSRLDILRKDNIVRCGVTIQRVLKSVDAAIENAQIQQTEIQVDVS
                     AAAIDTASRELAVLGGAEEETVQQSVNRSTIVIGGDTEVWPFIGEYWLDEVAYFRSQL
                     QSKCSE"
     gene            2243..>4205
                     /locus_tag="PKH54_09205"
     CDS             2243..>4205
                     /locus_tag="PKH54_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00114.1"
                     /translation="MKRYIASIAASILTTFVVMVGLPAVAQEAAQEGQAAEQGASDAP
                     KAVQGGKFTLEQAELMVGEAVKEKMAQISQMRQQQITNMKALLAKPDYRKDPARAPKV
                     IHMLAEAYWEESFYQYMKAREQWDKAYEQYDQGILKELPPEPTEDYTVSLEYYRQVLK
                     DFPDYPRYDQVLFYIGKGAMKEGKAKKNITLQKEGVNYLDRLVKNYPQSEWLPQAHLA
                     MAEFYFETNKLYYAQVNYETILNKYPKSSMYNYAQYKLSWVYFNMGEMEKAIDGFKMV
                     VKAVSTGKSKGVIEFRNQALNDLVVCFAEVENGWETALDYFKSVLPEDEAYVKMRALA
                     DLYVGQDKVLEAIELFRHFIERERTSKNIVEYYTIILSLYQNTNDTPNLDKVTLEVVD
                     YFDPRGTWATANKKDEDTVKEADTLVEKYVFYMANFYHMGAQKAEEKKETRLAKELYA
                     KASDKYSLYLTRFKDWERAYVVNFYYAEILYTELSDYQAAADQYNAVIARDTKGEYVE
                     EAALGVIYCYGELMDKEGLRKFAKSGKLETYQVDPKKAKKTFERTELHPLEASYVAAA
                     DKYVSLLTDLLTDPEVRKKNPKRGEAIPEIMFLAADVLYEHGQFEEAVKRLQTLFAYD
                     STSKYAAYAVFTLLDCYQRLNRWPQVEEWA"
BASE COUNT          958 a         1190 c         1258 g          799 t
ORIGIN      
        1 tcatggccaa ccttggcgcc ttcatcaagt tctgggtcac ggattacctg gcggtcggaa
       61 tcgattaccg tcattacatc tatccgaagg ccccggggtt tgacgcccag aacgtcagca
      121 agggcgaatg gaccaatggc gtcagctcgc tggccgaaat ttccttcggc ctgacttacg
      181 tcaccccggc cccgaagtga tgatgcggtc gctgttggcg attttgcggt ctctggccgc
      241 acggaactta acttgacggt gggaaaatga ctcgattttc attttcaaag ctgctgcttg
      301 ccgttgtgat taccggaact gtccttgtca gcggaccggc cggggcaatc acgaaggacg
      361 aagtaatcgt tcttgcaacg cagcttatgg ttcctgaagc cgacatcatc gcgacgatcg
      421 agaaggatcg gtcgattttc aagctggaaa tctccgatat tctcgagctt cgcaaggccg
      481 gtgtgcccga tgcggtaatc aagttcatga tgcagacccc gaccaggttc gcgcagcctg
      541 tccagcaggc ccccgcggtt cagcagcagc aggccgcggc ggttccggcg gcgccggtcg
      601 ttcgcgaaaa gacccccgag gaaattgaag ctgaacgacg ctttgccgcc gagcaggccg
      661 ccgcgcgcga ggcggaaatg aaggccgccg aggaagcccg tcgccgggcc tatgcctccg
      721 gaaagcttcg caagggcctt gaactcgccg accagggcga ctgggtcaat gcactgcaac
      781 tctttcagtc cttcctggcg gaagagaact atgccgacga ctcgattgaa gtctataacg
      841 ccaagtacgg aatggcggcg gcgctgacga acgcgggcct gtaccagagt gccgccaggc
      901 tccttgtcga agtacttctg cagggaacag agcggccgtt cttccaggaa gcgttccgca
      961 agctgcgcgt gcttcgccag agggtcaatt acaccccgca cgaactcgaa accctgacga
     1021 cattcccgat ggtggcgttc gacgcgacct tccaggtgga attcaactac gtcatggggc
     1081 agtatttcta tgacatcggc gattttgaac gcgcggtgac ccacctgaac caggtcgggg
     1141 atgccggtcc ggatgccgcc aaggcaaggt accttgtggg ccttgttcag gttcgcaacc
     1201 agatgtacaa gtcggccgtt gaatctttcc agagcgccgt caacctggcg gaaggcgcgt
     1261 tcaaggattc cggcgtgcgc gacctggcgt acatggcgct ggcccgaatc gcgtacgaag
     1321 cggaaaattt cgacgccgca atcttctatt acaagaaagt tccgaccgac agccccaggg
     1381 ccggcacggt gttcttcgaa ctggcctgga cgtatctgat gaaggccgac tacagtcgcg
     1441 ccctgggtac cttccatgcc atccgttcgc cgtatttccg cgatgcgttc ctgccggatc
     1501 tgtggatcct ggaagcgcgc gtctatggcg acctgtgcca tttcgacaag gcggaaaagg
     1561 ctcttggcat gttcgatcag gaaatcgtcg tgcaccagat cccgctgcgg cgtttcatgg
     1621 atgcccaggc gagcctggaa aactattacg acgccttcct taagtcggtt aacgagccgg
     1681 acgacaagag cctgccccag gttctgaccc tgccggtgat gtccgatgtc gagttttaca
     1741 acctgtaccg gacgatcaag cagattgaca aggaaatctc gatgattcag ggcaactccg
     1801 gcgccctggg tgcgttcgct gaagacatgc agtcccgtct cgatatcctg cgcaaggaca
     1861 acatcgttcg ttgcggcgtc acaatccagc gcgtgctgaa gtcagtcgat gcggcaatcg
     1921 agaatgcgca gatccagcag acggaaatcc aggttgacgt cagcgcggcg gccatcgata
     1981 cggccagccg tgaactggcg gttcttggcg gcgccgagga agagacagtc cagcagtcgg
     2041 tgaaccgcag cacgatcgtc atcggcggcg acaccgaggt gtggccgttc atcggcgagt
     2101 actggcttga cgaagtcgcg tacttccgga gccagctcca gagcaagtgc tcggagtagg
     2161 cgcccggccg gaattttgtg gcagtgcggt cgaacggccc gtagtggcct ttcggttgaa
     2221 tctttgacgg aatgagggac gaatgaaaag gtacatcgcg tccatcgccg cttcgatcct
     2281 gacgactttt gtcgtgatgg tcggtttgcc ggcggttgcc caggaggcgg cccaggaagg
     2341 ccaggctgcg gaacaaggcg cttctgacgc tccaaaggcg gttcagggcg gcaagttcac
     2401 actcgagcag gccgaactga tggtcggtga ggcagtcaag gagaagatgg cgcagatctc
     2461 ccagatgcgc cagcagcaaa tcaccaacat gaaggcgctg ctcgcgaagc cggattaccg
     2521 caaggatccg gcccgcgccc cgaaagtcat ccacatgctt gccgaagcct actgggaaga
     2581 gtctttctac cagtacatga aggcccgcga gcagtgggac aaggcctacg agcagtacga
     2641 tcagggcatc ctgaaggaac tgccgccgga gcccaccgag gactacaccg tttcgctgga
     2701 gtactaccgt caggttctca aggacttccc ggactacccg cgttacgacc aggtgctttt
     2761 ctacatcggc aagggcgcga tgaaggaagg caaggcgaag aagaacatca ccctgcagaa
     2821 ggagggcgtc aactatcttg accgcctggt gaagaattat ccgcagagtg agtggctgcc
     2881 gcaggcacac ctggccatgg ccgagttcta tttcgaaacg aacaagctgt attacgccca
     2941 ggtgaattac gagaccatcc tgaacaagta cccgaagtcg tccatgtaca actacgcgca
     3001 gtacaagctg tcgtgggttt acttcaacat gggcgagatg gaaaaggcga ttgacggctt
     3061 caagatggtc gtcaaggcag tctcgaccgg caagagcaag ggcgtcatcg agttcaggaa
     3121 ccaggcgttg aacgacctgg tcgtctgttt cgcggaagtc gagaacggat gggagacggc
     3181 gcttgattac ttcaagagcg ttctgccgga agacgaggcg tacgtgaaga tgcgcgccct
     3241 ggctgacctt tacgtcggcc aggacaaggt tctcgaggcg atcgaactgt tccgtcactt
     3301 tatcgaacgt gaaagaacct cgaagaacat cgttgagtat tacacaatca tcctttcgct
     3361 ttaccagaac acgaacgaca cgccgaacct ggacaaggtg acgcttgaag tcgtcgatta
     3421 tttcgatccc aggggcacgt gggcaaccgc caacaagaag gacgaggaca cggtcaagga
     3481 agccgacacg ctcgtcgaga agtacgtctt ctacatggcg aacttctacc acatgggcgc
     3541 ccagaaggcg gaagagaaga aggagacaag gctggccaag gaactttatg caaaggcatc
     3601 cgacaagtac agcctgtacc tgacccggtt caaggactgg gagcgcgcct acgtcgttaa
     3661 cttctactac gcggaaatcc tttacaccga gctctccgac taccaggccg ccgccgacca
     3721 gtacaacgcc gttatcgccc gcgataccaa gggcgaatac gtcgaggaag ccgcgctggg
     3781 cgtcatctac tgttacggcg agctgatgga caaggaaggc ctgcgcaagt tcgcaaagag
     3841 cggcaagctc gagacgtacc aggtcgatcc caagaaggcc aagaagacgt ttgagcggac
     3901 ggaactccat ccgctggagg cgtcgtacgt ggccgccgca gataagtatg tcagcctgct
     3961 gaccgatctg ctgaccgacc cggaagtcag aaagaagaac ccgaagcgcg gcgaggcgat
     4021 tcccgaaatc atgttcctgg ccgccgacgt tctttatgaa catggacagt tcgaggaagc
     4081 ggtcaagcgt cttcagacac tgtttgccta tgacagcacg tcgaagtacg ccgcatacgc
     4141 cgtcttcacc ctgctggact gctaccagcg cctgaacagg tggccgcagg tcgaggagtg
     4201 ggcca
//
LOCUS       DAOPPA010000287         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_22968_length_4193_cov_4.314886, whole genome shotgun sequence.
ACCESSION   DAOPPA010000287 DAOPPA010000000
VERSION     DAOPPA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22968_length_4193_cov_4.314886"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1239
                     /locus_tag="PKH54_09210"
     CDS             139..1239
                     /locus_tag="PKH54_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00115.1"
                     /translation="MNDATRILVTGAGGPGTVNLTRSLLMAPDPVFLLGTDASPYYIH
                     LALTHEKALVPRCDDGDAYIETISALCRKHSIDLVIPNSSVEMMALTRNSDAIPARMK
                     VPSLQAQETSASKWLTWSRWRDDGIPVPATMLINDADDVETAFRILPGDPIWFRGAGI
                     PGRGIGGAALPCRSVAEATGWIQFYSGWGGFIASEFLPGDNLTWLGIFDRGTLVASQG
                     RQRDAYVIPHVSPSGITGAPAICHTISRDDINELGPRCVLSIDPGFDGVAFVDFKCDA
                     DGNPRPTEINAGRFGTTNHFYTAAGFNFPWLMALIALGRDWPEPSGRFNVLKNDLYWV
                     RTLDAGPALLCRTRDGLVPYVPDSIFPPATPR"
     gene            complement(1226..2701)
                     /gene="gspN"
                     /locus_tag="PKH54_09215"
     CDS             complement(1226..2701)
                     /gene="gspN"
                     /locus_tag="PKH54_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HOD00116.1"
                     /translation="MAMEWTKNPGVIKGLKIGGVAVFALVSFVISIGYTLPEERIKSL
                     IEAKAADAGIVLSIADFDASGFGGVTIEGIRAELSPLVSEAADGTRLEQPRKLEIDRM
                     DLDVSLFSLLLGKPSFKLTVLDEGGHVGPARIKVLADSIDVEIEEIVDFPIPRGFPAG
                     PFPLSGVIRSGKGRIQWDRAGGIAGSEGAFELKAEKVTALKPVLNTKQAGAIGLTDIA
                     LGTFEASLIIDKRSNIAALKSSKGRSQTGGEGRVIYFETLKVDGKDIKAMVEGNSLIR
                     LASGRPLSKAQLTLEMAFAIEEAFFSKTNKDSTDTPNKFLKTLLDLDPRWKSAKSGEF
                     YGLMCTGTLDRPNCLPRRPSVRGGDFKAPSKSAEDDEEKPVRQKASRRKPVTTRQRPA
                     ATDESAGAAGGQAGNDAPAEPVQAPEPAAPTEPQPAVEFTPPPDGPTARRPGPPDTDV
                     DVTARVGGTAFIDRSESIRTQGEMRARPRIRLQEPGEPSEE"
     gene            complement(2725..3375)
                     /locus_tag="PKH54_09220"
     CDS             complement(2725..3375)
                     /locus_tag="PKH54_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00117.1"
                     /translation="MKISESFDAMTGRLSERERRLFGLLALAALLLLVGGAWFGVSTV
                     FSGIREDIEGDRKVLAELRNMAPQYLEKQARKRDLEAAVKNNTESVRVMANEILKDIE
                     LSAEIPGALGTRMSDIVSFEGKTVETPVELTKSKKAAKSKKKVTSSYVMEEQELKFKE
                     VPQNDLMRFLDAIEKSEALLFVTKIDMKRTFNDRNRVTASVVIATFRYREAAGRDE"
     gene            complement(3413..>4193)
                     /locus_tag="PKH54_09225"
     CDS             complement(3413..>4193)
                     /locus_tag="PKH54_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00118.1"
                     /translation="LNLRTGQFGSGGAAGLLREKAMFFSVALGVFLLLGAGWTATRYI
                     GLVGDRAAIEKQLVVLSSTILERQVDDFEGALAEVRAGSREELALFPAWTGAGTLEKI
                     TRAVLAAGPAGVRSVPAPGTGSQDDGSVEDTAGSGGESVEEDSAEVDPGHSMELEQIE
                     IGPKQAMIKGEAESIEKVDELIARLRGDACFHEIVTESTERVQFQRHQGWQKFRVRMN
                     VDCVGKDARKGAPATGAAATPGAAPASPPVQADSAAQVDGE"
BASE COUNT          813 a         1398 c         1139 g          843 t
ORIGIN      
        1 ccgctgcgtc cacatcgttc cgacaacggg ccgctgcggc cttgaatacc caaacaccga
       61 actgcagcag gccgaatcgt tcgagacacc caggggtaca tacgtcttct gcaaacactt
      121 tgaacttgag taggaaaaat gaacgatgcg accagaatcc tggtgaccgg ggccggcgga
      181 ccgggaaccg tcaaccttac gcgaagcctc ctgatggcac ccgatcctgt attcctgctc
      241 ggcaccgacg caagccccta ttacatccac ctggcactga cgcatgaaaa ggcgctggtt
      301 ccaaggtgcg acgacggtga tgcctacatc gagacaatct ccgcactgtg ccggaagcac
      361 tcgattgacc ttgtgatacc gaattccagc gtggaaatga tggccctgac caggaattcg
      421 gacgcgattc cggcgaggat gaaggttccg tcgctgcagg ctcaggagac ttcggcatcg
      481 aaatggctta cctggtcgcg atggcgcgac gacggcatac cggttccggc aacgatgctg
      541 attaacgacg cggacgatgt cgagacggcc tttcgcatcc tcccaggcga tccaatctgg
      601 tttcgaggcg ccggtattcc gggacgcgga atcgggggtg cggcactccc gtgcaggtcg
      661 gtcgcggaag cgaccggctg gatacagttc tattcaggct ggggcggatt catcgcttca
      721 gaatttcttc cgggagacaa cctgacctgg ctggggatct ttgatcgcgg cacccttgtt
      781 gcaagtcagg gacgacagcg tgacgcatac gtgatccctc acgtcagtcc aagcggaatt
      841 accggggccc cggccatctg ccacacaatc agcagggacg acatcaacga gcttggcccg
      901 cgatgcgtcc tttccatcga ccccggattc gacggcgtgg ccttcgtcga tttcaagtgc
      961 gatgccgatg gaaatccacg gcccactgag atcaacgccg gccggttcgg gacaacaaac
     1021 catttctaca ctgccgcagg gttcaatttt ccctggctga tggcacttat cgctcttgga
     1081 cgcgactggc cggaaccgag cgggcgcttc aacgtgttga agaacgatct ttactgggtc
     1141 aggactctcg atgcgggccc ggcactcctt tgcaggacca gggatggcct tgttccctac
     1201 gtaccggatt cgatctttcc accggctact cctcgctagg ctcgccaggt tcctgcagcc
     1261 ggattcgcgg ccttgccctc atctcgccct gcgtccggat actctcgctc cggtcaatga
     1321 aggcggttcc gccaacccgc gcggtcacat cgacatcggt gtccggcggc cccggcctcc
     1381 tggcggtcgg gccgtccggc ggcggcgtga actcaaccgc gggctgcggt tccgtcggcg
     1441 ccgcaggttc cggcgcctga accggttcgg ccggtgcatc gttcccggcc tggccgccgg
     1501 ctgcccccgc ggattcatcg gtcgcagccg gccgctgtcg cgtggtcacc ggcttacggc
     1561 gcgaagcctt ctggcgaacc ggcttctcct cgtcatcttc ggccgacttc gatggggcct
     1621 tgaagtcccc tccgcgcacg gaaggccttc gcggcaggca gttcggcctg tcgagagtgc
     1681 cggtgcacat caggccgtag aattccccgg acttggcgct cttccatcgc ggatcgagat
     1741 caagcagcgt cttgaggaac ttgttcgggg tatcggtcga gtccttgttt gtcttggaaa
     1801 agaaggcttc ctcgatcgca aaagccattt ccagcgtcaa ctgcgctttc gaaagaggac
     1861 gcccggaggc cagcctgatc agggaattcc cctcgaccat ggccttgatg tccttaccgt
     1921 caaccttcaa cgtctcgaaa tagatgaccc gaccttcgcc acccgtctgc gatcgcccct
     1981 tggatgactt caatgcggcg atattcgacc tcttgtcgat gatcagcgac gcctcgaagg
     2041 tgcccagggc aatgtcggtc aggccgattg cgcctgcctg cttcgtgttg agcacaggct
     2101 tcagcgccgt caccttttcg gccttcagct caaaggcacc ctcgcttccg gcaatgccgc
     2161 ccgcgcgatc ccactggatc cgtcccttgc ccgatcgaat gacccccgac agtggaaacg
     2221 ggcctgccgg aaatcccctt ggaatgggaa aatcgacaat ctcctcgatt tccacatcga
     2281 tggagtcggc cagcaccttg atgcgcgcag gcccgacgtg accaccttca tcaagcactg
     2341 tcagcttgaa ggacggtttt ccaagcagca gtgaaaagag gcttacatca agatccatcc
     2401 ggtctatctc aagcttcctg ggctgctcca gcctggttcc atcggccgct tcggacacca
     2461 gtggcgacag ctcggcccgg attccctcaa tcgtcacccc gccaaagccg gaagcgtcaa
     2521 aatccgcaat tgacagaacg attccggcat cagccgcctt tgcctcgatc agggacttga
     2581 tgcgctcctc gggaagcgtg tacccgatcg aaattacgaa cgaaaccagc gcgaaaacgg
     2641 ccacgccgcc aatcttcagg cccttgatta cgcctgggtt ctttgtccat tccatcgcca
     2701 tgggaatctc cggtatgaaa ccagtcactc gtcccgaccg gcggcttcgc gatacctgaa
     2761 ggtcgcgatt accaccgacg cggtgacgcg gttcctgtcg ttaaaagtcc ttttcatgtc
     2821 aatcttcgtc acgaaaagca gggcctcgga cttctctatt gcatccagga acctcatcag
     2881 atcgttctgc ggaacttcct tgaacttcaa ttcctgctct tccatcacat acgaggaagt
     2941 caccttcttc tttgacttgg ctgccttctt cgacttcgtc aactcaaccg gggtctcgac
     3001 ggtcttgcct tcaaagctga cgatgtccga catcctggtt ccaagggcgc cgggaatctc
     3061 cgcggacagt tcaatgtcct tcaggatttc attggccatc accctgaccg actccgtatt
     3121 gttcttgacc gccgcttcca gatccctttt cctggcctgc ttctcgagat actgcggagc
     3181 catgtttcga agctcggcaa gaaccttgcg atctccctcg atgtcttccc gtatcccgga
     3241 gaaaacggtc gaaacgccga accacgcccc gccgaccaga agaagcaggg cggccagggc
     3301 cagcaggccg aaaagccgtc tctccctctc ggaaagacga ccggtcatgg catcaaacga
     3361 ctccgaaatc ttcattcacg tcctccagcc ggaatctccg gcagaccaga catcactcgc
     3421 catcgacctg ggccgcagaa tcggcctgta caggcgggga cgcaggcgcg gcccccggtg
     3481 tcgcagccgc cccggtcgcc ggcgccccct tgcgggcatc ctttcccacg cagtcaacgt
     3541 tcatcctgac gcggaacttc tgccagccct gatgacgctg gaactgaaca cgctcggtgc
     3601 tttccgtgac gatctcgtgg aagcatgcat cgccccgaag gcgtgctatc aactcgtcaa
     3661 ccttctcgat ggactcagcc tcacccttga tcatggcctg cttcggcccg atctctatct
     3721 gctcaagctc catcgagtgg ccgggatcaa cctccgcgga atcttcctcg accgactcgc
     3781 ccccgcttcc ggcagtgtcc tcaacggaac cgtcatcctg ggagccagtc cccggggctg
     3841 gtacggaacg aacgccggca ggaccggcag caagcacggc cctggttatc ttctcaaggg
     3901 tccccgcacc ggtccaggcg ggaaacaggg ccagttcctc gcgcgagccg gcacgaacct
     3961 cggcaagcgc cccctcgaaa tcatccacct gccgttccag aatggttgac gacagcacca
     4021 caagttgctt ctcaatggca gccctgtcgc ccaccaggcc gatgtacctg gttgccgtcc
     4081 atccggcccc cagcagcagg aacaccccca gcgccacgga aaagaacatg gccttctcgc
     4141 gaagcagccc ggcggcccca cccgagccga actgaccggt tcgcaggttc aaa
//
LOCUS       DAOPPA010000288         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23352_length_4142_cov_4.219476, whole genome shotgun sequence.
ACCESSION   DAOPPA010000288 DAOPPA010000000
VERSION     DAOPPA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23352_length_4142_cov_4.219476"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKH54_09230"
     CDS             <1..697
                     /locus_tag="PKH54_09230"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD00119.1"
                     /translation="AALYVNHVMEGRPRDSARMLLEYLGPRHTMDEVLDTLEKGGIPY
                     LKPADPWETAGQLLARGCIGSVFQGAAEFGPRALGNRSTLSDPRPAEMKDRLNASVKF
                     REAFRPFAPSIHAHRANEYFEGCDFSPFMLRVFPTRPEKLDEARAVTHVDGGARVQTV
                     TREQNPNYYRLIESFEKQTGTPMVLNTSFNIRGEPIVNSPSDAVKCYLTTGMDFLVLE
                     DVLLVKNPSIIQS"
     gene            785..2890
                     /locus_tag="PKH54_09235"
     CDS             785..2890
                     /locus_tag="PKH54_09235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOD00120.1"
                     /translation="MGSSVREWELLDEIGRGGMGIVYRARHQILPGEWAIKVIRPELS
                     QDRELRQRFLSEIMVLKALRHPNIIEIETPFEESGQLFMPMEFLSGESLEVLCQQHQG
                     PWEPATAINIIGQAAAGLGYAHQRTPQVLHRDVKPANIQVLSDGRIKVLDFGLARTIG
                     DRSLTGTGMGVGTPAYMSPEVLEGRRATPQSDVFSLGVVLFRLLAGRLPHDMPPEDAT
                     LHAVLLAVARSIERGIRDVRTFVPGIPDQLADITMNTLAVDPATRPADCAELSRMLQS
                     LTSAPDPSSFTGREFSADSTHIGIDLGSIRGKASTGSIAPGQATSAPAVRPLNPPVRT
                     DRQPRPSPSPLPRPSPSPGPEVRDAMINTPRPAGTSRTPLFVAIGMAVAVLIGILAFL
                     AIDRSTGSGNDSTTGRHETGKTSVTASSGQRSSEPESPSSGAAPSEGSGSTGSTSRAG
                     GASDSARPMIDWVPIEGGSFMMGNDSGQADERPVHHVTVPSFQIARTEVTVRQYMKCV
                     SGGVCTMPHWDDGSCRVFNGADWPAAVLPEYMRGLDQPVLCVDYSQAATFSAWVGGRL
                     PSEAEWEFAARSRGANTTWPWGTTHPNCNLAVMDYGARGCGTSRTLDVCSRTAGNSAQ
                     GVCDLIGNAWEIVADNYNATYNGVPSDGSAYQNSARRFRVVRGGSWFDPAGITLRSTD
                     RFSVSGTYKSSAMGFRPVR"
     gene            2953..>4142
                     /locus_tag="PKH54_09240"
     CDS             2953..>4142
                     /locus_tag="PKH54_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD00121.1"
                     /translation="MKKSIFTVLAFVAVTVLYAAGSECIPRTQVVTTEEAKTGSAKTE
                     AIKTKATKTEDAKTEAAKIVGAGEVDKPTSTDVTPTHLKDGIDTLAARHKFKRVKAAM
                     YGVGADRYFIFEPESAGNAPLPLVLFVHGWGGMNPVNYGPWIEHLVLQGFIVVFPIYQ
                     VELSDPPKGVTKHATSAFKDALNRLGKDGHATADLDRVVAIGYSMGATISANMAAAAR
                     KLGLPRIRGLLLANPGDARHVAKGDLAKSIIGKLSTLPADTLVVTIVGDSDKLVKDRT
                     AIDIGKAICGIPAANRRMYVLHSASHGTWAATAGHGAPGAPDPRYDFGDDGSGFKPSI
                     PPAPWPTISKSVNNFDVFAYWPLADDLIDRVVRNRKVSRKDSGKKLFMGKWPDGKPFP
                     RASIA"
BASE COUNT          841 a         1305 c         1253 g          743 t
ORIGIN      
        1 cgccgccctt tacgtcaacc atgtcatgga aggccgcccg cgcgacagcg ccaggatgtt
       61 gctggaatac ctgggaccgc gccacacgat ggacgaagtc ctggacacgc ttgaaaaagg
      121 cggcatccct tacctgaagc cggccgaccc gtgggaaaca gctggccagc ttctggccag
      181 gggctgcatc ggcagtgtct tccagggagc cgccgaattc gggccgcgcg cgcttggcaa
      241 ccgcagtacc ctgtccgacc cgcgccccgc cgaaatgaag gaccgcctga acgccagcgt
      301 gaagttccgc gaggctttcc gcccgttcgc gccttccatt cacgcacacc gcgccaacga
      361 gtacttcgag ggatgcgact tctcgccgtt catgctgcgg gtcttcccga cgcgaccaga
      421 aaagctggac gaagcccgcg cggtcacaca cgtcgatggc ggcgcgcgcg tccagaccgt
      481 cacgcgcgaa cagaacccca attattacag gctgatcgag tcgttcgaaa agcagaccgg
      541 cacgccgatg gtcttgaaca ccagcttcaa catccgcggc gaaccgatcg tcaacagccc
      601 gtccgacgcg gtcaagtgct acctgaccac ggggatggat ttcctggtgc ttgaagacgt
      661 gcttctggtc aagaatcctt ccattatcca gtcctgaagc gccattccgt gctggaaaac
      721 cgacgttcga cgttgtatat gaataattct gtcaaatcag gtcgcatagt cctggaggcc
      781 ttgcatggga agttcggttc gggaatggga attgctggac gaaatcggtc gcggcggcat
      841 gggcatcgtc tatcgcgccc gccaccagat actgccgggt gaatgggcca tcaaggtcat
      901 ccggccggaa ctgagccagg atcgtgagct gcgtcagcgc ttcctgtcgg aaatcatggt
      961 cctgaaggcg cttcgccacc cgaacatcat cgaaatcgag accccgttcg aagagtcggg
     1021 gcagcttttc atgccgatgg aatttctttc cggcgaatcg cttgaggttc tgtgccagca
     1081 gcatcagggc ccatgggaac ccgcgacggc aatcaacatc atcggccagg cggcggccgg
     1141 ccttggatat gcacaccagc gcacgccaca ggttcttcac cgggatgtca agcctgcgaa
     1201 catccaggtt ctgtccgatg gcaggatcaa ggtcctggac ttcggcctgg caagaacaat
     1261 cggggatcgc tcgctgaccg gaaccgggat gggggttggg accccggcgt acatgtcgcc
     1321 ggaagtcctg gaaggccgca gggccacgcc gcagtccgac gtcttctcgc ttggcgtcgt
     1381 ccttttccgc cttctggcgg gacggcttcc acatgacatg ccgcccgagg acgccacgct
     1441 gcatgcggtc cttctggcgg tggccaggtc gattgaacgt ggaatccgcg atgtgcgcac
     1501 gttcgtgcca ggcattccgg atcaactggc cgacatcacg atgaataccc tggcggtcga
     1561 tcctgccacg cgaccggccg actgcgccga actttcgcgg atgcttcagt cccttacaag
     1621 cgcccccgac ccatcttcgt tcacggggcg ggaattttcc gcggacagca cgcacatcgg
     1681 aatcgacctc gggtcgatcc ggggaaaggc atcaaccggc tcaatcgcgc cgggacaagc
     1741 gacatcggcc ccggcggtcc ggccgttgaa cccgccggtg cgaaccgacc gccagccacg
     1801 cccctcgccc agccctttgc cacgcccttc gcccagcccc ggccccgaag tgcgggatgc
     1861 gatgattaat accccccgcc ctgccggcac ttcaaggacg cccctgtttg tcgcaatcgg
     1921 aatggcggtg gcggttctta ttggaatact cgccttcctt gccatcgatc gttcgacggg
     1981 atctggaaat gactcgacca caggccggca tgaaacaggc aagacatcgg tcacagcatc
     2041 ttccggacag agatcttccg aaccggagtc gccatcgtcc ggggccgcgc cttccgaagg
     2101 ctctggcagc accggttcca cctcacgagc cggcggtgct tcggattccg cgcgccccat
     2161 gatcgactgg gttcccatcg agggcggctc gttcatgatg ggaaacgact ccggccaggc
     2221 ggacgaacgt cccgtgcatc atgtgacggt accttcattc cagattgccc ggactgaagt
     2281 gacggtcagg caatacatga agtgcgtcag cggcggtgtc tgcacgatgc cacactggga
     2341 cgacgggtcc tgtcgcgtct tcaacggggc tgactggccg gccgctgtcc ttcccgaata
     2401 catgcgcggc ctggaccagc cggttctgtg cgtcgattat tcccaggcag ccacattctc
     2461 ggcctgggtc ggcgggcgcc ttccgtccga agcggaatgg gaattcgccg ccagaagccg
     2521 cggtgccaac acgacctggc cgtggggaac aacccatccg aactgcaacc tcgcggtgat
     2581 ggattacgga gcccgcggtt gcggaacatc caggacgctt gacgtatgct caagaacggc
     2641 cgggaactcg gcccagggag tctgcgacct gattggaaac gcatgggaaa tcgtcgccga
     2701 caactacaac gccacctaca acggggtgcc ttcggacgga agcgcctatc agaacagcgc
     2761 gcgcagattc cgcgtcgttc gcggcggctc gtggttcgat ccggcgggga tcacgctgcg
     2821 ttcgacggat cgtttcagcg tgtccggaac ctacaagagc tcggcgatgg gcttccggcc
     2881 ggtcaggtag gatgcccttg ttttacaggc cggccagacc gccggtcggg gggcctggaa
     2941 gtaaggtttg caatgaaaaa atcaattttc acggttcttg cttttgtcgc cgtgacagtc
     3001 ctttatgcag ccggcagtga atgcattccc aggacgcagg tcgtgaccac cgaggaagca
     3061 aagaccggat ccgcgaagac agaagcgata aagacaaaag cgacaaagac agaggacgca
     3121 aaaaccgaag ccgcgaagat cgttggcgcc ggcgaagtcg ataaaccgac ctcgaccgat
     3181 gtcacgccca cacacctgaa ggacggcatc gacacgctgg cggcacgcca caaattcaag
     3241 cgggtcaagg ctgccatgta cggcgtcggc gccgatcgat acttcatatt cgaacccgaa
     3301 tcggcgggca acgcgccgct tccacttgta cttttcgtac acggctgggg tggaatgaat
     3361 ccggtaaact acggcccctg gattgaacac ctggtgctgc agggcttcat cgtcgtgttc
     3421 ccgatttatc aggtcgaact gtcggatccg ccaaaagggg tcactaagca cgccacgtcc
     3481 gcgttcaagg acgcgctgaa ccgccttgga aaggacggcc acgcgaccgc cgacctggat
     3541 cgtgtcgtcg cgattgggta ctcgatgggc gccacgattt cggccaacat ggccgctgcg
     3601 gcccgaaagc tgggtctgcc acggattcgc ggcctgcttc ttgcgaaccc gggcgatgcg
     3661 cgtcacgtgg cgaagggcga cctggcaaag tccataatcg gcaagctttc gacacttccg
     3721 gccgacaccc tggtcgtcac aatcgtgggc gattcggaca agctggtcaa agacaggacc
     3781 gcaatcgata tcggcaaggc catctgcggc attcctgcgg cgaaccgccg gatgtacgtc
     3841 ctgcattcag caagccacgg aacctgggcg gcgactgcgg gacacggggc acccggggcg
     3901 ccggatccac gctacgactt cggggatgac ggcagcggct tcaagccgtc cattccgccg
     3961 gcgccgtggc cgacaatatc aaaatcagtc aacaacttcg acgtgttcgc atactggccg
     4021 ctggctgacg accttattga ccgggtcgtt cgaaacagaa aggtgtcacg caaggattcg
     4081 gggaaaaagc tgttcatggg aaaatggcct gacggcaagc cgttcccgcg ggcctcgatc
     4141 gc
//
LOCUS       DAOPPA010000289         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23353_length_4142_cov_3.947394, whole genome shotgun sequence.
ACCESSION   DAOPPA010000289 DAOPPA010000000
VERSION     DAOPPA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23353_length_4142_cov_3.947394"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKH54_09245"
     CDS             <1..462
                     /locus_tag="PKH54_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOD00122.1"
                     /translation="SCGKCIPCREGTRRMLEILQRITRGRRNEKDIEALERFQGVLYL
                     ERLGQVIKDTSLCGLGQTAPNPVLSTLRWFREEYEEHIYDRKCRAGACTELLRYSIDA
                     DKCIGCTLCARHCPTEAIMGAPKSPHYIIPDKCIGCGTCFSDCRHGAVIVE"
     gene            477..2198
                     /locus_tag="PKH54_09250"
     CDS             477..2198
                     /locus_tag="PKH54_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD00123.1"
                     /translation="MITIEVNNRKIEANPGEMLLDSLERAGIKVPTLCYMKDLFPTGA
                     CRMCVVEVEGVPGLVPSCAFPVRDGMKIQTHSERAVKARKTILELLLSNHPDDCLYCA
                     RNGDCHLQDLSEELGVRQRRYTGLRAELKPDTSSPSIVRDPNKCILCGKCVRVCEEVQ
                     GVSAIDFVGRGSKTKVAPAFNRGLNVSSCVDCGQCIIVCPTGALTEKSKFKDIFNAID
                     DPKKFVVVQHAPAVSVTLGELFGMAPGADVNGAMNAALRRLGFDRVFDTSFSADLTIM
                     EEASELFHRVKNGGTLPMFTSCCPAWIDFMEQNYPDMLANLSSCKSPQQMLGAVIKSF
                     YARKQGIDPKDIYVVSIMPCTAKKYEAQLPTHVSEGYPDVDAVLTTRELARIIKMRGL
                     HLNNLEPEGADTAFGERSSAGKLFGASGGVMEAAIRTAYFMGTGAELEELKVKAVRGL
                     DGVKEAHVKIGDLEIGVAVVNTLGNARKLVEEIRNGRSDLHFIEVMACPGGCIGGGGQ
                     PIGADLKAIKARLKALYEIDRDESLRVSHKNQQVKRLYEEYLGEPLGEKSHHLLHTTY
                     SRRDIDF"
     gene            2249..>4142
                     /locus_tag="PKH54_09255"
     CDS             2249..>4142
                     /locus_tag="PKH54_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD00124.1"
                     /translation="MALELVTTIGDRCKVCYTCVRECPAKAIRISGGQAEVIQERCIG
                     CGNCVQVCSQKAKAVMSSVEQVKELLERPGLKVSACVAPSFPVEFTDCSPEQFIGMVR
                     ALGFDLVNEVAFGADLVSEKYNELVKRHSGTKRRFIATACAAAVEYVEHLSPHLTESL
                     APLASPMVAMARYVHRMYGDDVAVVFIGPCIAKKAEAVELSRGQVDQVLTFGELREMF
                     RDAGIDPDKVIPTNFDPPFAKLGGIYPVSGGLVQTAKIDERLMSSEAAVVDGHNTFVE
                     AIREFESGAIQATVVEVMACNGCIMGPSVSSSVPLYSRRAMIADYIRHRMTTFDPEKW
                     VEDMYRYPDLDLSQTFKPEDRRMPTPSREEVNDILARLGKETAEDELNCGACGYDTCL
                     EHAIAIFQGLAEGEMCLPHTIDQLKSSVTELADVNKRLEHAQHALVQGEKLASMGQLA
                     AGIAHEVNNPLGVVLMYSHLLSEDCDVKPEMREDLKIITEQADRCKRIVSGLLNFARQ
                     NRVLLQPTVLRKFLESSLLGVQIPDNIKVEIQCPDHEAMAELDSDQLMQVFTNLLVNA
                     IEAMPDGGRIVLGCQFTERQVIFRIADNGVGIPPENAEKVFTPFFTTKPVGRGTGLGL
                     PVSYGIV"
BASE COUNT          899 a         1186 c         1292 g          765 t
ORIGIN      
        1 agctgcggga agtgcattcc atgccgcgag ggaaccaggc gcatgctgga aatccttcag
       61 aggattacca ggggacgccg caacgaaaag gatatcgagg cactggaacg cttccagggc
      121 gtgctctacc ttgaaaggct gggacaggtc atcaaggaca cgagcctttg cggactgggc
      181 cagacggcac cgaacccggt tctttcgacg ctgcgctggt tcagggagga gtacgaggaa
      241 cacatttatg acaggaagtg ccgggccggc gcgtgcaccg aactcctccg gtacagcatc
      301 gacgccgaca agtgcatcgg ctgcaccctg tgtgcacgcc attgcccgac cgaggcgatc
      361 atgggagccc cgaagagccc ccattacatc atcccggaca agtgcattgg ctgtggaacc
      421 tgtttcagcg attgtcgtca cggcgccgtg attgtggagt aaccgggagg ttccagatga
      481 ttaccattga agtcaacaac cgtaagatcg aggcaaatcc cggcgaaatg ctgctggatt
      541 cgcttgaaag ggcgggcatc aaggtaccga ccctgtgcta catgaaggat ctgttcccga
      601 ccggcgcgtg ccgcatgtgc gtcgtcgagg tcgagggtgt cccaggcctg gttccgagct
      661 gcgccttccc cgtccgcgac gggatgaaga tccagaccca ctcggaacgg gcggtcaagg
      721 cacgcaagac gattctcgaa ctgctgcttt caaaccaccc ggacgactgc ctttactgcg
      781 caaggaacgg cgactgccac ctgcaggatc tttccgagga acttggggtc cgtcagcggc
      841 gctacaccgg cctgcgggcg gaactgaagc cggacaccag cagtccgtcc atcgtgcgcg
      901 acccgaacaa gtgcatcctt tgcgggaaat gcgtccgcgt ctgcgaggaa gtccagggcg
      961 tttcagccat cgacttcgtc ggcaggggat ccaagacaaa ggtggccccg gcattcaacc
     1021 gcggattgaa cgtgtccagt tgcgtcgatt gcggacagtg catcatcgtc tgcccgacag
     1081 gcgcgctgac ggaaaagagc aagttcaagg acatcttcaa cgctatcgac gacccgaaaa
     1141 agttcgtggt tgttcagcac gccccggccg tctccgtgac gctgggcgaa ctctttggaa
     1201 tggccccggg ggccgacgtg aacggtgcca tgaacgcggc tcttcgccgt cttggctttg
     1261 accgcgtatt cgatacttcg ttctcggccg accttaccat catggaagag gccagcgaac
     1321 tgttccaccg ggtcaaaaac ggcggaacgc ttccgatgtt cacatcgtgc tgtccggcct
     1381 ggatcgactt catggaacag aactaccctg acatgctggc aaacctgtct tcgtgcaaga
     1441 gcccccagca gatgcttggg gcggtgataa agagcttcta tgccaggaag cagggaatcg
     1501 acccgaagga catctatgtg gtgtccatca tgccctgcac cgccaagaag tatgaagcac
     1561 agcttccaac ccacgtcagc gaaggatacc cggacgtcga tgcagtcctt accacgcgcg
     1621 aacttgcacg catcatcaag atgcgcggac ttcacctgaa caacctggaa ccggaaggcg
     1681 ccgacactgc tttcggggaa aggagttcgg cgggcaagct gttcggggca agcggcggcg
     1741 tcatggaggc cgccatcagg acggcgtact tcatggggac cggggccgag cttgaggaac
     1801 tgaaggtcaa ggccgtgcgc gggctcgatg gcgtgaagga agcccacgtg aagatcggcg
     1861 acctggaaat cggcgtcgcg gtcgtcaaca cgcttggcaa tgccaggaag ctggtcgagg
     1921 aaatccggaa cggacgatcc gatctccact tcattgaagt catggcatgc ccaggcgggt
     1981 gcatcggtgg cggcggccag ccgatcggtg cggatctgaa ggccatcaag gccagactga
     2041 aggcccttta cgaaatcgat cgcgacgaaa gcctgagggt ttcccacaag aaccagcagg
     2101 tcaagcgtct gtacgaggag tatctgggcg agccgcttgg cgaaaagagc caccacctgc
     2161 tgcatacgac gtattccagg agggacattg atttctaacg gccgttgggc gatgttaatg
     2221 tcctgacggc actggcggag ctgctgaaat ggcgcttgaa ctggtcacca caattgggga
     2281 tcgctgcaag gtctgctaca cctgcgtgcg ggaatgtccg gccaaggcaa tacgcatcag
     2341 cggaggtcag gccgaggtca tccaggagcg gtgcatcgga tgcggaaact gcgtccaggt
     2401 atgcagccag aaggccaagg ccgtcatgag ttccgtcgaa caggtgaagg aactgctgga
     2461 aagaccgggg ctgaaggttt cggcgtgtgt tgccccgagc ttcccggtcg agttcaccga
     2521 ctgcagtccg gagcagttca tcggcatggt tcgcgcactt ggtttcgacc ttgtcaacga
     2581 ggtcgcgttc ggcgcggatc tggtgtccga gaagtacaac gaactggtga agcgccattc
     2641 cggaacaaag cggcggttca tcgcgacggc ctgcgccgcc gcggtggaat acgtcgaaca
     2701 cctgtcgccc cacctgactg aatcgctggc accgcttgct tcaccgatgg tggcgatggc
     2761 ccggtacgtc caccggatgt acggcgatga cgtggcggtg gtattcatcg gcccgtgcat
     2821 cgcgaagaag gccgaggcgg tcgagctttc ccgggggcag gtcgatcagg ttctgacctt
     2881 cggcgaactg cgcgagatgt tccgggacgc cggcatcgat cccgacaaag tcattccgac
     2941 caacttcgat cctccatttg ccaagctggg cgggatatac ccggtcagcg gggggcttgt
     3001 ccagaccgcc aagatcgacg agcgactgat gtcttccgaa gcggcggtgg ttgacggaca
     3061 caatacgttc gtcgaggcaa tcagggaatt cgagtccggg gctatccagg ctaccgttgt
     3121 cgaggtcatg gcctgcaacg gatgcatcat ggggccatcc gtttcaagtt cggttccgct
     3181 ttactcgcga cgggccatga ttgccgatta catccgccac aggatgacga cgttcgatcc
     3241 cgagaagtgg gtcgaggaca tgtaccgcta tcccgacctg gatctttcgc agaccttcaa
     3301 gcccgaggat cggcgcatgc cgaccccaag ccgggaggaa gtcaacgaca tccttgccag
     3361 actgggaaag gagaccgccg aagacgaact gaactgcggg gcctgtggtt acgatacatg
     3421 cctggaacat gccatcgcca tcttccaggg cctggccgaa ggtgagatgt gccttccaca
     3481 caccattgac cagctcaaga gctcggtcac tgaactggcg gacgttaaca agcgactgga
     3541 acatgcccag cacgcccttg tccagggcga aaaacttgca agcatgggtc agcttgcggc
     3601 cggcatcgcc cacgaggtca acaacccgct tggcgttgtt ctgatgtatt cacacctgct
     3661 gtccgaagac tgcgatgtga agcccgagat gagggaggat cttaagatca tcacggagca
     3721 ggcagacagg tgcaagcgca tcgtgtccgg actgctgaac ttcgcaaggc agaaccgggt
     3781 gcttctgcag ccgaccgtgc tgcgcaaatt tctggaatct tcgttgctcg gggtgcagat
     3841 tccggacaac atcaaggtgg aaatccagtg cccggatcac gaagcgatgg cggagctgga
     3901 ctccgaccag ttgatgcagg tctttaccaa cctgctggtc aacgcgatcg aggccatgcc
     3961 ggacggtggc cggatcgtcc ttggctgcca gttcaccgag cgacaggtaa tcttccggat
     4021 tgcggacaac ggggttggca ttccccccga aaatgccgaa aaggtgttca cgccgttctt
     4081 cacgaccaag ccggtcggac ggggaaccgg gctgggactg ccggtaagct acggcatcgt
     4141 ca
//
LOCUS       DAOPPA010000290         4108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23602_length_4108_cov_3.069578, whole genome shotgun sequence.
ACCESSION   DAOPPA010000290 DAOPPA010000000
VERSION     DAOPPA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23602_length_4108_cov_3.069578"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKH54_09260"
     CDS             <1..597
                     /locus_tag="PKH54_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOD00125.1"
                     /translation="VVTQKINMRKGRMINYGSHGTGRVRLEFKIPSRCLIGYHDEFLT
                     DTRGTGIMSSYLVGYEEYCGDFTSRFSGSIVADRAGVAVAYALWGLEDRGRLFLVPGD
                     PFYEGMIVGEHNRPNDIDVNPAREKKLSNMRAAGRDDNILLSPASPLTLEQALQFIKD
                     DEMVEVTPKSVRLRKLFLSEHKRHNLNGIKKLAAMAEG"
     gene            complement(628..1467)
                     /locus_tag="PKH54_09265"
     CDS             complement(628..1467)
                     /locus_tag="PKH54_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD00126.1"
                     /translation="MNQLTEGAMAALNAATKLGIIATVGPDGLPHITLMSTLEAKDPT
                     HLMFGQFSEGKSKEHLRQNPLMAFVVVTLDKRLVGGHARYTHATREGEDYEHFNAQPL
                     FRYNTYFGINTVHYLDLLDATEVQPLPMARIIPSAIATRLGKGAFADRTRPRILKHPA
                     QAIFNGLGNLKFLAYVDDDGMPRIFPLIQCQAADSRRLAFATGAFGKHIDRLVPGRPV
                     AVFGMTMQMESVLARGPFVGFHRRGFIRLGLIDIDWVYNSMPPCHGQIYPEVPLKAFE
                     NQY"
     gene            complement(1483..2448)
                     /locus_tag="PKH54_09270"
     CDS             complement(1483..2448)
                     /locus_tag="PKH54_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOD00127.1"
                     /translation="MADGLVLEIQRMSTEDGPGLRTTVFLKGCPLRCEWCHNPESISP
                     RPQVQWVEAKCIGCGSCASACGHQALEWSPGLVRIDREKCVGAGDCVDACPATALEMW
                     GRRMSADELVRELVKDRVYFEKAGGGVTISGGEATAQPGFCLEVLKGLKAAGIGTAID
                     TCGLCRVETLDSLLPLADIVLFDLKLMDSTAHRLYCGTGNEGILENARHVARSILADE
                     TAAGSTVLWIRTPLIPGATATAENIGAIGRFIADELGAAVSRWDLCAFNHLGRDKYRR
                     LGMDWPYRGTPLMTAAELEEMGAVARASGVSPGIVRVSGATRLDD"
     gene            complement(2466..>4108)
                     /locus_tag="PKH54_09275"
     CDS             complement(2466..>4108)
                     /locus_tag="PKH54_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOD00128.1"
                     /translation="VSFGRFDQYLLPYWRKSSADGMDREFGKELLKCFWMHCNTAYDA
                     QIRSGNQGITAGFGQLITLSGVGPGGADMTNEFTYAVLEVIDEMSPILEPKPNVRLHR
                     NSPPELMDRVVQMIADSQGAPFLLNFDERSMAGMIRQARMAGMQDRINEDNVHEYAPV
                     GCLENTMVGNDRSGTVDNNLNLVKAVELALTGGRDLLPYRDPITGVESKITQDGPSTG
                     DSSMFKDFEEFWDAYAAQTSYIVERCVGVHEASEAVRARFFPTPYVSCLVSGCAASGR
                     DVTQGAAEISLTTLEAVTFATTVDSLLAVKYLVFDKGVCTMKQLLQALRDNWVGHEVL
                     QAHAKNRAPKYGRDDDQADGMAVRVMELWCDHAWRHSTAATGRRYRPGMLSWNYWAGD
                     GYVVVATPDGRPRGQFLSNAICPVNGADINGPTSNSNSVGKALGGRAIEGEGDLAGYF
                     NCLPNGASHTITFNPAFIRDPEHREKFKSFLYGYIQNGGTALQINILDPDMLRDAQKH
                     PQDYRHLLVRVTGYNAYFVGIGRELQDEVIARMSHQRI"
BASE COUNT          780 a         1353 c         1212 g          763 t
ORIGIN      
        1 gtcgtcaccc agaagatcaa catgcgcaaa ggccggatga tcaattacgg aagccacggt
       61 accggccgcg tcaggctcga gttcaagatc cccagcagat gtctgatcgg ataccacgac
      121 gaatttctga ccgacaccag gggaaccgga atcatgagct cgtacctggt cggttacgag
      181 gaatactgcg gagacttcac cagccgcttc agcggctcga tcgtcgccga ccgcgcgggc
      241 gttgccgtgg cctacgcgct gtggggtctt gaggacaggg gccggctgtt cctggttccg
      301 ggcgacccct tctatgaggg catgatcgtc ggcgaacaca atcgtccgaa cgacatagac
      361 gtgaacccgg ccagggaaaa gaaactcagc aacatgcggg ccgcgggtcg cgacgacaac
      421 atcctgctgt caccggcttc gcccctgacc cttgaacagg cgctgcagtt catcaaggac
      481 gacgagatgg tcgaagtcac ccctaagtcc gtccgcctgc gcaaactgtt cctgtccgaa
      541 cataaacgcc acaacctgaa cggtatcaag aaactggccg cgatggccga gggctgaaga
      601 accgcgagac caccgggaaa ttgtagttca gtattgattc tcgaacgcct tcagcgggac
      661 ttcgggatag atctgaccgt gacagggcgg catcgagttg tagacccagt cgatgtcgat
      721 caggcccagg cggatgaagc cgcggcgatg gaacccgacg aacgggccgc gcgccaggac
      781 gctttccatc tgcatcgtca tgccgaacac cgcgacgggc cgaccgggaa ccagccgatc
      841 gatatgcttg ccaaacgcgc cggtggcgaa agccagccgc ctgctgtccg cggcctgaca
      901 ctggatgagc ggaaaaatcc gcggcatgcc gtcatcgtcg acgtaagcca gaaacttcag
      961 gtttcccaga ccgttgaaaa tggcctgggc cgggtgtttc aggatacgcg gccgcgtccg
     1021 gtcggcaaac gcgcccttgc ccagcctggt ggcgatggcg gacgggatga ttctggccat
     1081 cggcaggggt tgaacctcgg tcgcgtccag cagatccaga tagtgcacgg tattgatccc
     1141 gaagtacgtg ttgtaacgga acaacggctg ggcgttgaaa tgctcataat cctcgccctc
     1201 gcgggtcgcg tgggtgtatc gggcatgccc cccgaccagc cgcttgtcca gggtgacgac
     1261 aacgaacgcc atcaaagggt tctggcgcag gtgttccttg cttttccctt ccgagaactg
     1321 cccgaacatc aggtgcgtcg gatccttcgc ctcaagggtt gacatcaggg tgatgtgagg
     1381 gaggccgtcg ggaccgaccg tcgcgatgat ccccagtttg gtcgcggcgt tcaacgcggc
     1441 catggcgcct tcggtcaact gattcatgcg gaaacctcca gtctagtcgt ccaggcgcgt
     1501 tgccccgctg acacgcacaa tcccgggtga aacgcccgaa gcacgcgcga ccgcgcccat
     1561 ttcttccagt tcggccgccg tcatcagggg cgtgccgcga tatggccagt ccatccccag
     1621 ccgcctgtac ttgtcgcgcc ccaggtgatt gaaagcgcaa agatcccagc gcgacaccgc
     1681 cgcgccaagt tcgtccgcga tgaaacgacc gatggcgccg atgttttccg ccgtggccgt
     1741 cgcgcccggg atcaaaggcg tccggatcca caaaacggtg gaccctgccg ccgtttcgtc
     1801 cgccaggatc gaccgcgcca cgtgacgcgc gttttccagg atgccttcgt tgccggtccc
     1861 gcagtaaagt cgatgcgcgg tcgagtccat aagcttcagg tcgaacagaa cgatgtcggc
     1921 caatggcaac agcgagtcca gcgtttcaac gcggcacagc ccgcacgtgt cgatggccgt
     1981 gccgatgccg gccgccttca ggcccttcag cacctcgaga cagaatcctg gctgggcagt
     2041 cgcctcgcca cccgagatcg tcacgccccc gcccgctttt tcgaaataca cccgatcctt
     2101 gaccagttcg cggacaagct cgtccgcgga catgcggcgg ccccacatct ccagtgccgt
     2161 tgccgggcag gcatccacgc agtcacccgc ccctacacac ttttcacggt caatccgcac
     2221 cagaccgggc gaccattcca acgcctgatg tccgcatgcc gacgcgcacg acccgcaacc
     2281 tatgcacttc gcctccaccc actggacctg gggacgcgga ctgatgcttt ccggattgtg
     2341 acaccactcg caacgcagcg gacagccctt caaaaacacg gtggtccgca gaccggggcc
     2401 gtcctcggtc gacatgcgct gaatctccag gaccagaccg tccgccatcg aaatccccct
     2461 gcccgtcaga tgcgctgatg gctcatgcgc gcgatgacct catcctgcag ttccctgccg
     2521 atgccgacga agtacgcgtt gtacccggtg accctgacca gcagatgacg gtagtcctgc
     2581 gggtgtttct gcgcgtcgcg cagcatgtcc gggtccagga tgttgatctg cagcgcggtt
     2641 ccgccattct ggatgtaccc gtacagaaac gacttgaact tttcgcggtg ttcggggtcc
     2701 cttatgaacg ccgggttgaa cgtgatggtg tggctggcgc cgttgggaag acagttgaag
     2761 tagccggcca ggtcgccttc accctcgatc gccctgccgc caagcgcctt tccaacggaa
     2821 ttgctgttcg atgtcgggcc gttgatgtcg gcgccgttga ccgggcagat tgcgttggac
     2881 aggaactgcc cccgtggccg gccatccggt gtagccacca ccacatagcc gtcgcccgcc
     2941 cagtagttcc agcttagcat cccgggccgg tagcgtcgcc ccgttgcggc ggtgctgtgg
     3001 cgccaggcat ggtcacacca cagttccatc acgcgcacag ccatcccgtc ggcctgatcg
     3061 tcgtcccgac cgtacttggg cgcgcgattc ttcgcgtgcg cctgaagcac ctcgtgcccg
     3121 acccagttgt ctcgcagggc ctggagcagt tgcttcatgg tgcagacacc cttgtcgaac
     3181 acaaggtact tgaccgccag cagcgaatcg accgtggtcg cgaacgtcac cgcttcaagg
     3241 gtagtcaggc tgatctcggc cgctccctgc gtgacatccc tgccacttgc agcgcacccg
     3301 gacaccaggc aggacacgta gggcgtcggg aagaatctgg cgcgcaccgc ctcggacgcc
     3361 tcgtgaacac caacgcaccg ttcgacgata tagctggtct gagcggcgta cgcgtcccag
     3421 aattcctcga aatccttgaa cattgacgag tcgccggtcg acgggccgtc ctgcgtgatc
     3481 tttgattcaa cacccgtaat cgggtcgcga tacggcagca ggtcgcgccc tccggtcagg
     3541 gccagttcga ccgccttgac caggttcagg ttgttgtcga ccgtgcccga gcggtcgttc
     3601 ccgaccatcg tgttttccag acacccgacc ggcgcgtact cgtgaacgtt atcctcgttg
     3661 atccgatcct gcatgccggc catgcgggcc tgcctgatca tcccggccat cgaccgctcg
     3721 tcgaagttca gcagaaacgg cgcaccctgg ctgtcggcga tcatctgcac gacgcgatcc
     3781 atcagttccg gcggggaatt gcggtgcagg cgcacgttcg gtttcggttc aaggatcggc
     3841 gacatctcgt cgatcacttc cagcactgca tacgtgaact cgttcgtcat gtccgcgccg
     3901 ccagggccga ccccggaaag cgtgatgagc tgtccgaaac cggccgtgat gccctggttt
     3961 ccgctgcgga tctgcgcgtc gtacgccgta ttgcagtgca tccagaaaca cttcaacagt
     4021 tccttgccga attcgcgatc catcccgtcc gcgctggact tgcgccagta cggcagcagg
     4081 tactgatcga accggccgaa ggacacgc
//
LOCUS       DAOPPA010000291         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23705_length_4093_cov_4.536157, whole genome shotgun sequence.
ACCESSION   DAOPPA010000291 DAOPPA010000000
VERSION     DAOPPA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23705_length_4093_cov_4.536157"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKH54_09280"
     CDS             complement(<1..644)
                     /locus_tag="PKH54_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00129.1"
                     /translation="MPFLHRLNVAAIQIAFILPLVAPSRAPAAEIVDPPGSATALLAT
                     AGRVAANGCGPESGLECPDVPMFVSGLMSMGEFLACGVVCQSLESRRQLPGEALFLCA
                     EAASRLGDMVPAERWLSAAVGSAGSGDDAAAIALKAADMLIAGARFKSAGMFLEMAAD
                     KGGDSPELQRRIFSNCLDAGDAGCATSLGQKLAVADLGPGTAIFVIREFFSHDL"
     gene            complement(702..2105)
                     /gene="purF"
                     /locus_tag="PKH54_09285"
     CDS             complement(702..2105)
                     /gene="purF"
                     /locus_tag="PKH54_09285"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOD00130.1"
                     /translation="MKSIHDECGVFGIFGHEGASTLAYLGLHSLQHRGQESAGIATWD
                     GRKMFLYRQMGLVGDIFSHEVLAGLPGDCAVGHVRYSTAGSSSLLNAQPINCQYAGGD
                     LALCHNGNIVNANTLKWELMNRGHLFQCSSDSEVLLQLVAAHTAPRLHDRVAAMMESA
                     RGAYSLVLLGRDEMVAVRDPYGFRPLVVGNLKGATVVASETTALSLIGATYEREVEPG
                     EILVVTRGGRESHFPVRAPRLAQCVFEHIYFARPDSIVFGRQVYEVRSLFGRQLAREA
                     PVPNGDVVIPVPDSGTSAANGFSKESGIPFDMGLIRSHYVGRTFIEPEQKIRHFGVKL
                     KLAAVEATIRNRVVVVVDDSIVRGTTSAKIVQMVRDKGAREVHLRISSPPTRWPCYYG
                     IDTPCREELIAARLHDNRLIARELTADSVGYLSIEGMHRVVSQGSGNGWCDACFSGRY
                     PVPPEAGYSSHTREAKS"
     gene            complement(2177..2743)
                     /locus_tag="PKH54_09290"
     CDS             complement(2177..2743)
                     /locus_tag="PKH54_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOD00131.1"
                     /translation="MFDNFRVGDKAVYPAHGVAEVLGIESREVSGSKQTFYMLKLMDS
                     EHRIMIPLSKAMSVGLRQVIAEEEVPQVFGLLKAKPQRLTRQNWNRRYRGYLEKLKTG
                     SVFDVAEVLRDLCLLRNEKPLSFGEKRLLDTARVLLVEEISVATNGSQDDIACELEAI
                     FPMPLEGSRSPDEESADESGGDSQAQPG"
     gene            complement(2776..3723)
                     /gene="bamD"
                     /locus_tag="PKH54_09295"
     CDS             complement(2776..3723)
                     /gene="bamD"
                     /locus_tag="PKH54_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOD00132.1"
                     /translation="MHNFSKLLIFVAIFAGAGCASQKPQVQARSTVSTTLEVARRAYH
                     DGMNSMKEGDFDAAFKSFSLASRAPSHVVYSRLARLRLADTLYYQNMFEEASKAYVAF
                     INTNTSNPNLHYAYFRLADSKVQSISGDFFLVPPSDRRDQKPVRSALKSIQSFVANFP
                     DSPYCSQVLKMRRRMVDIVSSFEMEVARFNMTRKKPEGALLRIEQLMKDIPSTRDSDK
                     VRYAYIGALAAAGRHEMAVRESTDYLDRFTTARNRDDVQKIMAGSRTAIQKAAEAEKA
                     RQEKEAAEAARAAGPAGEPKPADGATPGDGGEPADDQAK"
     gene            3858..>4093
                     /locus_tag="PKH54_09300"
     CDS             3858..>4093
                     /locus_tag="PKH54_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00133.1"
                     /translation="MAEGMSGIACVCDPSNRGRWMTAFESAGAGRLGIRVIFPGSGSF
                     VPEPGRWLVFLDTCPIGIESILSRFPIGDSTVATG"
BASE COUNT          820 a         1289 c         1160 g          824 t
ORIGIN      
        1 aacagatcat ggctgaagaa ttcgcgaatc acaaatatgg ccgtgcccgg gcccaagtcg
       61 gccacggcaa gtttctggcc cagggatgtc gcgcaaccgg cgtcaccggc gtcaaggcag
      121 ttggaaaata tccttcgctg aagttccgga ctgtccccgc ccttgtcggc ggccatttcc
      181 agaaacatgc ccgccgattt gaaacgcgcg ccggcaatca gcatgtcggc tgcctttaag
      241 gcaattgccg cagcatcgtc gccactgccg gccgaaccga ccgccgccga caaccagcgc
      301 tcggcgggaa ccatgtcgcc caggcgcgag gccgcctcgg cgcacaggaa tagtgcttcg
      361 ccggggagtt ggcgcctgga ctcgagtgac tggcacacga ctccgcaagc cagaaactcg
      421 cccatcgaca tcaaaccgct gacgaacatg ggaacatctg gacactccag cccggattcg
      481 gggccgcatc cattcgcggc gacccgcccc gccgttgcca gaagagccgt cgccgatccg
      541 ggcggatcga cgatttccgc ggcaggcgcg cgcgatggcg cgaccagcgg cagtatgaat
      601 gcgatttgaa ttgcggcgac gttcagtctg tgaaggaaag gcaccgtgtc acccccgctt
      661 tctggcgcgt gacggccttc aggggcgtca cgggaaacct gtcatgactt ggcttcgcgg
      721 gtatgcgatg agtatccggc ctccggcgga accgggtaac gtccggagaa acacgcatca
      781 caccagccgt tgccggatcc ctgggatacg acgcggtgca taccctcgat tgacagataa
      841 ccaacggagt cggccgttaa ttcccgcgcg ataagcctgt tgtcatgaag cctggccgca
      901 atcagttcct cgcggcatgg cgtgtcgatg ccgtagtagc agggccaccg tgtcggcggc
      961 gaagaaatcc ggagatgaac ctcgcgggct cccttgtccc gaaccatctg aacaatcttc
     1021 gccgacgtcg tgccgcgaac aatcgaatcg tcgaccacga ccacgacccg gtttcttatc
     1081 gtcgcctcga ccgcggccag tttcaatttg acgccgaaat ggcgaatctt ctgttcaggc
     1141 tcgatgaaag tgcgtccgac atagtggctg cggataagac ccatgtcgaa cggaatgccc
     1201 gattcctttg agaatccgtt ggccgccgac gtgccggagt ccggcaccgg aatcactaca
     1261 tcgccgttcg gaactggcgc ctcgcgcgcg agctggcgtc cgaatagcga cctgacctca
     1321 taaacctgac gcccgaaaac gatgctgtcg gggcgagcga agtagatgtg ttcaaacacg
     1381 cactgggcca gacgtggcgc ccgaaccggg aaatggcttt cccggccgcc gcgcgtgact
     1441 accagaatct cgccgggctc gacttcccgc tcgtatgtgg cgccaatcaa cgagagagcc
     1501 gtggtttccg atgccacaac cgtcgcgcct ttcaggttgc ccacgacaag gggacggaag
     1561 ccgtacggat ccctgaccgc gaccatttcg tcccgaccaa gcagaaccag cgaatacgca
     1621 ccccttgcgc tttccatcat ggccgcgacg cgatcgtgca gcctgggcgc ggtatgggcg
     1681 gccacaagct gaagcagcac ctcgctgtcg gacgaacatt ggaacaggtg cccccggttc
     1741 atcaattccc atttcagtgt gttggcgttg acgatgttgc cgttatggca gagtgccaga
     1801 tcaccgcccg catactggca attaatcggc tgcgcgttaa gcagcgacga tgagccggcc
     1861 gtcgagtatc tgacatggcc gacggcgcaa tcgcccggca gtccggcaag aacctcgtgc
     1921 gaaaatatgt cgccgacaag gcccatctgc ctgtaaagaa acatcttgcg gccgtcccac
     1981 gtcgcgatgc cggcgctctc ctggccccgg tgctgcaacg aatgaagccc cagatacgcc
     2041 agtgtcgatg cgccttcgtg gccgaaaatt ccaaacactc cgcactcgtc atgaatgctt
     2101 ttcaagctgg acctcttgta actgtagctg tggtttccag tccgggcgcc ggcgaaagcc
     2161 gggccctgtt ccggcgtcaa ccgggctgcg cctgtgaatc ccccccggat tcgtccgccg
     2221 actcttcgtc cggcgatctc gagccctcga gaggcatcgg gaaaatggct tcaagttcgc
     2281 aggcgatgtc gtcctgcgaa ccgttggtgg cgaccgaaat ttcctccacc agaagaaccc
     2341 tggcggtgtc gagcaacctc ttctccccga aggaaagggg cttttcgttg cgaagaaggc
     2401 acagatcccg aagaacctcg gcaacgtcga agacggagcc ggtcttcagc ttttccagat
     2461 acccacggta acgccggttc cagttctggc gggtaagccg ctgcggcttg gccttcagga
     2521 ggccgaaaac ctgcgggacc tcttcctctg cgatgacctg cctcaggccg accgacatgg
     2581 ccttcgacag cggaatcatg atccgatgct cggaatccat cagcttcaac atgtagaaag
     2641 tctgcttgct tccgctgact tcacggcttt cgattcccag aacctcggcc accccgtgcg
     2701 cgggatatac ggccttgtca ccgactctga aattatcgaa catcgaaaac ctccaaggat
     2761 atccggggat acccattatt ttgcctgatc gtcggccggc tcgcccccat cgccgggtgt
     2821 ggcaccgtca gccggtttcg gttcccccgc cggccccgct gccctggccg cctccgcggc
     2881 ctccttttcc tgtctggcct tttcggcctc ggccgccttc tggattgctg tccttgaacc
     2941 ggccattatc ttctggacgt cgtcgcggtt tcgcgcagtc gtgaaacgat ccagatagtc
     3001 cgttgattcc ctgaccgcca tctcgtgcct tcccgccgcc gccagggcgc cgatataagc
     3061 gtacctgacc ttgtcggaat cccgtgtgga aggaatatcc ttcatcaact gttcaatgcg
     3121 cagcagcgcg ccttccggct tcttgcgggt catattgaac cgcgcgactt ccatctcgaa
     3181 tgacgagacg atatcgacca tccgccgccg catcttcaga acctgcgagc aatacggact
     3241 gtcgggaaaa ttggccacga aggactgaat cgatttcagc gcggatcgca ccggcttctg
     3301 atcccgcctg tccgatggcg gaaccagaaa gaaatcaccc gagatggact gcaccttgct
     3361 gtcggcgaga cggaaatatg cgtaatgcag gtttggattg cttgtattcg tgttgataaa
     3421 ggccacgtag gccttgctgg cctcctcgaa catgttctgg tagtacagcg tgtccgcaag
     3481 gcggagacgg gccagccttg aatatacgac gtgggaaggc gcgcgcgacg caaggctgaa
     3541 actcttgaag gcggcatcga aatcgccctc tttcattgag ttcattccgt cgtgatatgc
     3601 ccgcctggca acctcaagag ttgttgaaac ggtacttctg gcctgaacct gcggcttctg
     3661 ggacgcgcat cccgcaccag cgaagattgc aacaaaaatc agtaatttcg aaaagttgtg
     3721 catttagtaa gaaacacctc agccgaaagt atcttatccc gtcgaacccc cgaatgtcaa
     3781 ccgccacgcc cccgcacttt tgaaaatcac gcccctgtcg tagcatatgc cggacttaac
     3841 ccgcgtcggt gagcttaatg gctgaaggca tgtcgggaat cgcttgcgtc tgcgatcctt
     3901 cgaaccgcgg ccggtggatg acggcctttg aatccgccgg cgcggggcgg cttggaatcc
     3961 gggtcatctt tcccggttcc ggttctttcg ttccggaacc tggtcgatgg ctggttttcc
     4021 tggatacgtg cccgattggg attgagtcga tcctgtcacg ctttccaatc ggggactcga
     4081 ctgttgccac ggg
//
LOCUS       DAOPPA010000292         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23707_length_4093_cov_3.992571, whole genome shotgun sequence.
ACCESSION   DAOPPA010000292 DAOPPA010000000
VERSION     DAOPPA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23707_length_4093_cov_3.992571"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKH54_09305"
     CDS             complement(<1..259)
                     /locus_tag="PKH54_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00134.1"
                     /translation="MNRGRNSAPRWLTPVARVLVVICLAAVSLAALAGMGGCGQQMST
                     VDRLDQTLSYYHIHLTSGEIQRAAAYVDRDHIDDFLSRHDPE"
     gene            430..837
                     /locus_tag="PKH54_09310"
     CDS             430..837
                     /locus_tag="PKH54_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD00135.1"
                     /translation="MKIAVATSNGNILASGEPSTARGFVIYEVAFGTARRVEFRPNRR
                     GHRSAVSDVRARDIGHTLSDCAMILAGAVSAHTRDYLENTGIEVVITDETIVDRAAAL
                     LALNQLPDQSRVDPEDLEIRQGEPVVFEDGFDA"
     gene            830..1588
                     /locus_tag="PKH54_09315"
     CDS             830..1588
                     /locus_tag="PKH54_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD00136.1"
                     /translation="MPDPAVDLSGVHVRFNDRTILQDCNLRLGAGLIHGIVGSGGSGR
                     STLLKVIGTVIPADAGRVTILGHDIDQCGGDVLASIRSRIGFQFQNLALFDSFDVIDN
                     VLFAVTGGDPDGAEVADRERAVELLDSVGLGDFLHRHAGQLSGGMQRRLAIARAFAPR
                     QAMLHIFDDPVAGLDPVTSSRILGLIASRCRSGTGTTAVISTHEIDALLAICDLVHVI
                     HQGSIIFSGTPAQAVGNRQEVVARLFRDPVLHRG"
     gene            1609..2361
                     /locus_tag="PKH54_09320"
     CDS             1609..2361
                     /locus_tag="PKH54_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD00137.1"
                     /translation="MTSNAATTVLETTGAMAVTSWTALTALVRGRAGRRQALRQIAIV
                     SVDSLPVLVLISLLSGFVMVLESASYVESLGAFSLMGWAFGLVTLSQLGPVLVALMFA
                     ARAGARTTAELGAMAVTGQLDALRLLGIDPVGYLAAPRFLAMIVMLPLMTALCDAIAI
                     GAGAVGAAVVVGLGPALFLQSIAQGGLMDEFLLGIARTFLFGGAIAAVSCTFGFRVRG
                     GAAGAGRAVNDCVVVSALAVFVIDLIFTIAVL"
     gene            2369..3103
                     /locus_tag="PKH54_09325"
     CDS             2369..3103
                     /locus_tag="PKH54_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD00138.1"
                     /translation="MNRRVNEIGLLIQAIVAMTRRPPPLRRIVREVFSCGNRSIVFVC
                     VTMAFFGMILVYEGCMQALALIPDLSSAGPATIRSTIRVFGPLMAGLMVSFRVGAGIA
                     AEAGTMVVTEQDGALLVSGSSPAEWLVAPRLVACIIAVPALTVVGCLAGILAGGALGV
                     GWFGIHPSDFFSLSMVDRGDAAVCAAKSLLNGMAIPVFAAAAGLNTRGGSEGVGVAAT
                     SAVVRSCVAVAVIEFLVSTVARVAGA"
     gene            3196..3993
                     /locus_tag="PKH54_09330"
     CDS             3196..3993
                     /locus_tag="PKH54_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD00139.1"
                     /translation="MAASDAEAIAIDIQDVGRSFGSQRVLDRVSTRFMTGRATFVVGP
                     SGCGKSLLMKMLVGLETPDSGRILVFGKHVATMNAAELNGLRRRVAFVPQHPALFDSL
                     TVGANIALPLVFHRIQDEAAARSTAVRLLSLFNLADRADLLPGQISQSDRKIVSILRS
                     MACGPECLVLDEPTTGLDSPSRDAVFSMIREIGLVDSGIETLIVVSHDMQSTMAIADR
                     IVFLYKGVVRHDTSPAGFLTATATDPIAAQFLAGAADGPMNTEADEA"
BASE COUNT          644 a         1247 c         1289 g          913 t
ORIGIN      
        1 gttccgggtc gtgccgggat agaaaatcgt cgatgtgatc gcgatcgacg tatgcggcgg
       61 ctcgctgaat ctcgcccgat gtcaggtgga tgtggtagta gctcaaggtc tgatcaaggc
      121 gatcgacggt gctcatctgc tgcccgcagc cgcccattcc ggccagcgcc gccagcgaga
      181 ccgcggcgag gcagatgacc acaagaaccc tcgccaccgg cgtcaaccat cgtggggcac
      241 tgttccgacc gcggttcatt cgatttctcc agtccgtcaa ccagacgggc gaaagattaa
      301 catcccggcg ggtctgatgc aaagccgctt gcacgcgccc attcaccttg aatcacagga
      361 acatccgtgt ttgtacttga aaacagccgg cggggaacca ataattcagc ccgacggagg
      421 tggtcatcga tgaaaattgc tgtcgccaca tcgaacggca acatacttgc ttccggcgag
      481 ccctctaccg cccgcggctt cgtaatctac gaggtggcct tcggcacggc ccgccgcgtc
      541 gagttccgcc ccaaccggcg cggacaccgc agcgcagtgt ccgacgttcg agcccgcgac
      601 atcgggcata cgctgtccga ctgcgccatg atccttgccg gcgcggtcag cgcgcacacc
      661 cgggactacc ttgagaatac cggcatcgaa gtcgtcataa cggacgaaac cattgtggac
      721 agggccgcgg cccttcttgc gcttaaccag cttcccgacc agtccagggt cgatcccgaa
      781 gaccttgaaa tcaggcaggg cgaacctgtc gtgttcgagg atggattcga tgcctgatcc
      841 ggctgtcgat ctttcgggtg ttcatgtccg gttcaacgat cgcaccatcc ttcaggactg
      901 caatttaaga ctcggggccg gcctgattca cggcattgtc ggctccggcg gctccggccg
      961 ttcgaccctg cttaaggtta tcggcaccgt cattccggcg gacgccgggc gagtaaccat
     1021 ccttgggcac gacattgatc agtgtggcgg ggatgtcctg gcgtcgatcc gatcccggat
     1081 tggtttccaa ttccagaatc ttgctctctt cgactcattc gacgtgattg acaacgtctt
     1141 gttcgccgtg accggcggtg atcccgacgg ggccgaagtc gccgaccggg agcgcgccgt
     1201 tgaactgttg gattccgtcg ggcttggcga ctttctgcat cggcatgccg ggcagttgtc
     1261 cggcggcatg cagcgccggc ttgccattgc ccgagccttc gcgccgcgcc aggccatgct
     1321 ccacatcttc gatgatccgg tcgccggcct cgacccggtg acatcttcga gaatcctcgg
     1381 attgattgcg tctcgctgca gatccggcac tggcacgacc gccgtcatct ccacccatga
     1441 gatagacgct cttttggcga tatgcgacct tgttcatgtt atacatcagg ggtcgatcat
     1501 cttttcgggt accccggcgc aggcagtcgg caatcgacag gaagtggtgg cccgcctttt
     1561 ccgggatccg gttcttcatc gcggctgaat ttgggctggg gaatgggatt gaccagcaat
     1621 gcggcaacaa cggttcttga aacgaccggc gcgatggccg ttacttcgtg gacggcgttg
     1681 accgcgcttg tccgtgggcg tgccggtcgt cgccaggctc ttcgccagat tgcaatcgtg
     1741 agtgtcgatt cccttccggt acttgtcctg atttcacttc tttccggctt cgtgatggtg
     1801 cttgaatcgg caagttacgt cgaatcgctc ggggcgttca gtctgatggg atgggccttc
     1861 gggctggtca ccctctcgca gctgggccct gtcctggtgg ccctgatgtt cgcagcgcgc
     1921 gccggtgcgc gaacgacggc ggaactgggc gccatggccg ttaccggcca actcgacgcg
     1981 ttgcgactgc tcgggatcga tccggtcggg tacctcgcgg ccccaaggtt cctggcgatg
     2041 attgttatgc ttcccctgat gacggcgctc tgcgacgcga ttgcgatcgg ggcgggtgcc
     2101 gtcggagcgg cggtggtcgt cggtcttggc cccgcgctct tcctccagtc gattgctcag
     2161 ggcgggctga tggatgagtt ccttctgggg atcgcccgca cctttctttt cggcggggcg
     2221 atcgccgcgg tttcctgcac attcggcttc cgggttcgcg gcggggccgc cggcgcgggc
     2281 cgggcggtca acgactgcgt tgtggtgtcg gcacttgccg tgtttgtgat tgacctgatt
     2341 tttactattg ccgtgcttta agggaaacgt gaatcgacgc gtgaacgaaa tcgggctgtt
     2401 gattcaggcc atcgtcgcga tgacgcgccg tccgccgccg cttcgtcgca ttgtccgcga
     2461 ggtgttttcc tgcggcaatc gttcgatcgt gttcgtgtgc gtgaccatgg ccttcttcgg
     2521 gatgattctt gtctatgaag ggtgcatgca ggcactggcg ttgattccgg atctttcatc
     2581 cgccggcccg gcgaccatcc gttcaacaat ccgcgttttc ggcccgctga tggccggcct
     2641 gatggtttca ttccgcgtcg gggccggtat cgccgccgag gccggaacaa tggtggtaac
     2701 cgaacaggac ggggcgctgc tggtttccgg gtcgagcccg gccgaatggc ttgtggcgcc
     2761 ccgccttgtc gcgtgcatca tcgccgttcc cgcgctgacg gtggtcggct gtcttgccgg
     2821 aatcctggcc ggcggcgccc tcggggtggg ctggttcggg attcacccat cggatttttt
     2881 cagcctctcg atggtggatc gcggcgacgc cgccgtatgc gcggccaagt cgcttttgaa
     2941 cggcatggcc attcccgtgt ttgccgccgc cgccgggctg aacacccgcg gcggttcgga
     3001 aggtgtcggc gtggcggcga caagcgccgt tgttcgatcc tgcgtggctg tcgccgtgat
     3061 tgaattcctg gtttcgacgg tcgcccgggt cgccggggca tgaagccgcg gggggcttgt
     3121 tcggatggcg cgtttgtcac ggatccggtg gatgatgccg agttcccgac cggttcctgt
     3181 gttacggaga tactgatggc agccagcgac gcggaagcaa tagcaatcga catccaggat
     3241 gtcgggcgtt cgttcggttc ccaacgagtt cttgatcggg tatccacgcg tttcatgacc
     3301 gggcgcgcca ctttcgtggt cggtcccagc ggttgtggaa agtcccttct gatgaagatg
     3361 ctcgtcgggc tcgagacacc cgattccgga agaatcctgg tcttcggcaa gcatgtcgcg
     3421 accatgaatg cggctgaact gaacggcctt cgccgaaggg tggccttcgt tccgcagcac
     3481 ccggctcttt tcgactcgtt gaccgtcggt gccaatatcg ccctgcccct ggttttccac
     3541 cggatccagg acgaggccgc ggcccgctcg acggccgttc gtcttctgtc actgttcaac
     3601 cttgccgacc gcgccgacct gttgcctggc cagatcagcc agtcggacag gaagattgtc
     3661 tccattcttc gctcgatggc atgcgggccc gagtgcctgg tgcttgacga gcccaccacc
     3721 ggccttgatt ctccgtcaag agacgccgtg ttctcgatga tccgggaaat cggcctggtc
     3781 gattccggca tcgaaaccct gatagttgtt tcacacgaca tgcaatcaac catggcgatt
     3841 gccgaccgca ttgtctttct gtataagggt gtggttcggc acgacacgtc cccggccggc
     3901 ttcctgacgg cgacggcgac ggatccgatc gcggcccaat tccttgccgg ggccgcggat
     3961 ggaccgatga acacagaggc tgacgaagca tgaacgaacg aaccaggatg gaaatcaagg
     4021 tcggggcact ggttctggtc tcggtcgccg ccatccttgg attcgtggcg gttctgggga
     4081 acgtcagctt cgg
//
LOCUS       DAOPPA010000293         4091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23718_length_4091_cov_3.408573, whole genome shotgun sequence.
ACCESSION   DAOPPA010000293 DAOPPA010000000
VERSION     DAOPPA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23718_length_4091_cov_3.408573"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKH54_09335"
     CDS             <1..937
                     /locus_tag="PKH54_09335"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265875.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOD00140.1"
                     /translation="MFFEGDRITSMRKMIMAETEPERRAALAELLPYQREDFYGIFKA
                     MKGYPVTIRLLDPPLHEFLPGDEKNQKDFSAKTGTPISKIQERLEDLHELNPMLGHRG
                     CRLGNTYPEITEMQARAIFEAACDVAKEGLEVNPEVMVPLVGHVNELKVQEQIIRKTA
                     TEVFAEKGITVKYLVGTMIEVPRAAVTSAQIAEVAEFFSFGTNDLTQMGFGMSRDDYA
                     KFMPIYIANGILKHDPFATLDDGIFELMKLGVNGGRTTRTDLKCGICGEHGGDPDSVT
                     LCDQAGLNYVSCSPFRVPVARVAAAQANIAARKGN"
     gene            954..2303
                     /locus_tag="PKH54_09340"
     CDS             954..2303
                     /locus_tag="PKH54_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00141.1"
                     /translation="MTYGSGHANLNPGAHRPLPASFVGGAARSVALLLSISVIFHAAC
                     IDNYRQITPDPGQFAWPGAIVADPAGDFVYVVSTNFDSRYTGGTLIPIQVRPEDGGAP
                     VPVSKAAVEIGPFGGDAAVSLLGDGTTRIWVPIRDGDQLDLVDSVRDASNLPELECLA
                     QESREKAEPGADGDAYAFHRCRGDHVIDLRDYDFVDEEGTTFWANDAYGISVGNQMRT
                     FDGDVVNPVYVSGIVGSAVAVFTVAADGSLQWRATLPITEGAHSIMEYVVDEFTRVLW
                     ITNRSVNLIDIIKLRFDRFGNVREIPMPSVSADLLGSSGDYFREMVRSGDGRLLYAAF
                     RSPSSLTIFEILDGGGLRYRGLIPLDGTPSGVAIFNDNGKEVVYVTESSLDFVYVIDP
                     ASMAVVSKVFVGAAPSGIAIADGRAFVANFEDSTVSWFGVDSLRPDYHKVGIVEPRD"
     gene            2322..>4091
                     /locus_tag="PKH54_09345"
     CDS             2322..>4091
                     /locus_tag="PKH54_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00142.1"
                     /translation="MKRSFSALIVLATLAGLVGLVACADEKAYSGSILSRPVDFAVTC
                     VSADRSFMPVKDCGATDGLAVSWILDGDLDGVFVSSLPDGDHFDFDPVLPGYSPMPVR
                     QDAVAIEPSPDSRYVFVASNGGVESKPALTLIDGDFFSMAGAISSVDLPCPLSDMTVM
                     EDAEAPAGYAIAALLSCTSGSQVWRLEPPAGDLRSLEGSFVSMGLVAEIPGRARWIST
                     GEGRVMVVSADLPQPEYLHWDHLVALDGLVPGATSVTTVPVVNDSRIVPKYRSEGVAA
                     GCGDGQAQSVASGYVSGRPAVMPGGDFIYVPMAVPSGIAVFDADLARVDTHIPVPGLD
                     MTDTTIPDQPWNIANMLAGWLGYADIRMDSPALGVTFVQTEDGLRALAWLYGGQIVRV
                     LPEASDTVPWPHRAENDLLEVESSAYMPVVTWQGAELERAILTRTDLPSFGGTQVVIP
                     KVDDEKYVYYGITFNGDIEAELPEVWSATYEGVIPGAEGGCGRLMYDTGEGWFSTWTL
                     ATTSYAGFCDLGVQAADGDYVGDTVEIDLPWDTECGELAGSTMEFRLVRVSGGQLRLG
                     PLTASGAQISIPEGCQYKDVNWRV"
BASE COUNT          734 a         1185 c         1285 g          887 t
ORIGIN      
        1 catgttcttc gaaggcgacc gcatcacgtc gatgcgcaag atgatcatgg ccgagacgga
       61 acccgagcgt cgcgcggcgc ttgcggaact gctgccgtac cagcgcgagg acttctatgg
      121 aatcttcaag gcgatgaagg gctatccggt caccatccgc ctgctggatc cgccgctgca
      181 tgaattcctg cccggcgacg aaaagaacca gaaggatttc agcgcaaaga ccggcacccc
      241 gatttccaaa attcaggagc gcctggaaga cctgcatgaa ctgaacccga tgctgggtca
      301 ccgtggctgc cgcctgggca acacgtaccc ggaaatcacg gagatgcagg cccgcgcgat
      361 tttcgaggcc gcctgcgacg tcgcgaagga aggcctggaa gtcaacccgg aagtcatggt
      421 tccgctggtc ggccatgtca acgaactgaa ggtccaggag cagatcatcc gcaagaccgc
      481 caccgaggtg ttcgccgaga agggcatcac cgtcaagtac ctggtcggca cgatgatcga
      541 ggttccacga gccgcggtta cgtcggccca gatcgccgag gtcgccgaat tcttcagctt
      601 cggaaccaac gacctgaccc agatgggctt cggcatgtcg cgtgacgact acgccaagtt
      661 catgccgatc tacatcgcaa acggcatcct gaagcacgac cccttcgcca cgcttgacga
      721 cgggattttc gagctgatga agctgggcgt taacggtggt cgcacgaccc gcacggatct
      781 gaagtgcggt atctgcggcg aacacggcgg cgatccggat tcggtgaccc tttgcgacca
      841 ggcgggtctg aactatgtca gttgcagccc gttccgcgtt ccggtcgccc gcgtcgcggc
      901 ggctcaggcg aatatcgccg ccaggaaggg caactagcaa tggaaggtgc aaggtgacat
      961 acggatcggg ccacgctaac ttgaatcccg gcgcgcaccg gccgcttccg gcgtcgttcg
     1021 tcggcggcgc ggcccggtcc gtggccttgc ttctttccat ttccgtcatc tttcatgcgg
     1081 cgtgcatcga caattaccgg caaataaccc cggatccggg ccagttcgcc tggcctggcg
     1141 ccatcgtcgc agaccccgcg ggtgatttcg tatatgtcgt ctcgaccaac ttcgattccc
     1201 gatacaccgg cgggacgctg attccgatac aggtcaggcc ggaggacggc ggcgccccgg
     1261 ttcctgtttc gaaggcggcc gtggagattg ggcctttcgg cggtgatgcc gcggtgtccc
     1321 tgcttggaga cggcacgacc aggatctggg tcccgatccg tgacggcgac cagctcgatc
     1381 tggtcgattc cgtccgtgac gcatcgaacc ttcccgagct ggagtgcctt gcacaggaaa
     1441 gccgcgaaaa ggccgagcct ggcgctgacg gcgacgcata cgcttttcat cgatgtcgtg
     1501 gggatcacgt catcgatttg agggattacg atttcgtcga tgaggaaggc acgactttct
     1561 gggccaacga tgcttacggc atttccgttg gaaatcagat gcggaccttc gacggcgatg
     1621 ttgtgaatcc cgtctatgtg tcgggaatcg ttggaagcgc cgtggccgtt tttacggtcg
     1681 ccgccgatgg ttccctgcaa tggcgagcca cgcttccaat caccgagggc gcccattcga
     1741 tcatggagta cgtcgtcgat gagttcacgc gcgtattgtg gattaccaat cgcagcgtca
     1801 acctgatcga catcatcaag ctgcgtttcg atcgcttcgg gaatgtccgc gagatcccga
     1861 tgccttccgt gtcggccgac ctgctcggtt cttccggcga ttacttccgc gagatggttc
     1921 gttccgggga cggaagactt ctttacgcgg cgttccgttc cccatcatcg ctgacaattt
     1981 tcgaaattct cgatggtggc ggcttacgtt accgtggttt gattccactt gatggtaccc
     2041 cgtccggggt ggcgattttt aacgacaacg gcaaggaagt cgtctatgtg accgagtcct
     2101 cgctggattt tgtgtatgta atcgatcccg cttcgatggc ggtggtttca aaggtctttg
     2161 tcggcgcggc gccttccggt atcgcgattg ccgacgggcg cgcctttgtc gcgaacttcg
     2221 aggactcgac agtaagctgg ttcggcgtcg attccttaag gcccgactat cataaggtcg
     2281 gtatcgtcga gccccgggac tgagccggct ggaggctgga gttgaaaagg tctttttctg
     2341 cgttgatcgt tctggcgacg ctggcaggtc ttgtcggcct ggtggcctgc gcggacgaaa
     2401 aggcatacag cggctccatc ctgtcacgac cggtcgattt cgccgtgacg tgcgtttccg
     2461 ccgaccgcag tttcatgccc gtcaaggatt gcggagcaac cgatggtctg gccgtttcat
     2521 ggatcctgga cggcgacctg gacggcgtct tcgtaagctc gctcccagat ggtgatcatt
     2581 tcgatttcga cccggttctt ccgggatatt ccccgatgcc ggttcgccag gacgccgttg
     2641 cgatcgaacc gtcgccggac tcaaggtacg ttttcgtcgc ttcaaatggc ggcgttgaat
     2701 cgaagccggc cctgaccctt atcgacggtg atttcttttc catggccggg gcgatttctt
     2761 cggttgatct cccgtgtccc ctttcggaca tgacggtaat ggaggacgcc gaagcgcctg
     2821 ccgggtatgc aattgcggca cttctgtcgt gtacgtcagg atcccaggtg tggcgtcttg
     2881 agcccccggc cggtgatctc cggtcgcttg aaggttcgtt cgtttcaatg gggctggttg
     2941 cggagatccc ggggcgtgcc agatggatat cgacgggcga gggaagggtg atggtggttt
     3001 cggccgatct tccccagcct gagtaccttc actgggatca tcttgtcgcg ctcgacgggc
     3061 ttgttccggg cgcgacatca gtcaccacgg ttcctgttgt caacgacagc cggattgtgc
     3121 cgaagtaccg atccgaaggc gtggccgccg gttgtggcga tggccaggcg cagtcggtgg
     3181 cgtcaggtta cgtgtcggga cggcccgccg tgatgcccgg cggcgacttc atatacgtcc
     3241 cgatggccgt accctccggc attgccgttt ttgacgccga tctcgccagg gtcgatacgc
     3301 acatcccggt tccaggtctc gacatgaccg ataccaccat tccggatcag ccctggaata
     3361 tagccaacat gctggcgggc tggcttggct atgcggacat aaggatggat tcgccggctc
     3421 tcggcgtcac gttcgtccag accgaagacg gccttcgcgc actggcctgg ctttatggcg
     3481 gccagatcgt acgggtattg ccagaggcat ccgataccgt tccgtggccg cacagggccg
     3541 aaaacgatct gctcgaggta gagagttcgg cgtacatgcc ggtcgtgacc tggcaggggg
     3601 ctgaacttga gcgcgccatc cttaccagga cggatcttcc ctcgttcggc gggacgcagg
     3661 tcgtgatccc gaaggtcgat gacgagaagt acgtgtatta cggcattacg ttcaacggcg
     3721 atatcgaggc cgaacttccc gaagtctggt ctgcaacgta cgagggcgtg attccgggtg
     3781 cagagggcgg ctgcggaagg ctcatgtacg acaccggcga ggggtggttc agtacctgga
     3841 cgcttgccac cacttcgtat gccggattct gtgatctcgg ggttcaggcg gccgacggtg
     3901 actacgtcgg cgataccgtg gaaatcgatc ttccatggga caccgaatgc ggtgaactgg
     3961 ccggttccac gatggaattc cggctcgtgc gggtttcagg cggtcagttg cggcttggcc
     4021 cgctgacggc gagcggcgcg cagataagca ttcccgaagg ttgtcagtac aaggatgtca
     4081 actggcgggt a
//
LOCUS       DAOPPA010000294         4086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23767_length_4086_cov_3.870752, whole genome shotgun sequence.
ACCESSION   DAOPPA010000294 DAOPPA010000000
VERSION     DAOPPA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23767_length_4086_cov_3.870752"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PKH54_09350"
     CDS             <1..1079
                     /locus_tag="PKH54_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00143.1"
                     /translation="NGEKYQGGRTETDFMRSICNAFKVEKPAACANIPEPKKIAVTVL
                     SDKRCTKCFPDRILGQLKNMFPGLEPKTLDYADAEGKALYDSLKDQGVKLLPAFLFEA
                     AVVEDAGFNQIKRFIVDAGQYKLLQVGAKFDPTAEICDNQADDDGDGKIDCEDDGCAG
                     QAECRPEVVKQLDVFVMSQCPFGVKALNALPEIIDAFKEDGITLNINFIADEMPDGTF
                     KALHGQPEVDENIRELCAIKNYGKDHKYMDYILCRNKDIRNAEWKTCAVNGIDPAVME
                     KCSTGEEGKKLHSENIKIAKALEIGASPTWLANNKFKFSGIAPEQIKKQFCDHNKELK
                     GCAKTLSGEAKVQPGAAPAPSCGG"
     gene            1225..2594
                     /locus_tag="PKH54_09355"
                     /pseudo
     CDS             1225..2594
                     /locus_tag="PKH54_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
     gene            2617..3366
                     /locus_tag="PKH54_09360"
     CDS             2617..3366
                     /locus_tag="PKH54_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD00144.1"
                     /translation="MQLKDAVLVGLDTETTGLDTRRDRVFEIGLVTYRSGDVVENWGR
                     LMDPLVELDQKVVETTGVTTEEVSGQPVFAEVVDEIEKRISGQILVGYNIIDFDLPML
                     ATEFERLGRQMPPFQVIDVLVFARQLARGGRHRLGDMAARFGVEMDTAHRATADADAT
                     VRLLYALAPSLPDDVEKLLVLQSQWSAQQQSKRAMWRDRGDGGALIGSGQAAAVAHDD
                     KVALGPAYLYGDERDPLKAFINQYADQTVRR"
     gene            3435..>4086
                     /locus_tag="PKH54_09365"
     CDS             3435..>4086
                     /locus_tag="PKH54_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00145.1"
                     /translation="MSSRLPFLSRLASLAVALCAAGCFSESPLPECDTLADCPVNQGY
                     NACIDGWCYRTQACADRPVVAGDGCCALTEGDRSEDTDCMLADLALSCRDPAGPSVDD
                     DGNIYVTCLMPLTDGTRNVVLRRLDTSGNLSVPLVAGIGTVAFSPVMGKGSTVMVQFG
                     AGLARFSTVNLGDAGGQSTGPAAARVASNGGSDAFRGIGGWPTLDGKVVLIDEEKNI"
BASE COUNT          855 a         1169 c         1278 g          784 t
ORIGIN      
        1 tgaacggcga gaagtaccag ggtggccgca ccgagaccga cttcatgcgt tcgatctgca
       61 acgcattcaa ggttgaaaag ccggccgcct gcgcgaacat ccccgagccg aagaagatcg
      121 ccgtcaccgt tctgtccgac aagcgctgca cgaagtgctt ccccgacagg atccttggcc
      181 agctgaagaa catgttcccc ggacttgaac cgaagaccct cgattacgcg gatgcggaag
      241 gcaaggccct ttacgacagc ctgaaggatc agggcgttaa gcttctgccg gcgttcctgt
      301 ttgaagccgc ggtcgtcgag gacgccggtt tcaaccagat taagcgtttc atcgttgatg
      361 ccggtcagta caagctgctg caggttggcg cgaagttcga tccgaccgcc gaaatctgtg
      421 acaaccaggc cgacgatgat ggcgatggca agatcgactg cgaagacgac ggctgcgcgg
      481 gccaggccga atgccgcccc gaagttgtca agcagctcga cgtgttcgtc atgtcccagt
      541 gcccgttcgg cgtgaaggcg ctcaacgccc ttcccgaaat catcgacgcg ttcaaggaag
      601 acggaatcac cctgaacatc aacttcatcg ccgacgaaat gccggacggc accttcaagg
      661 cgctccacgg ccagccggaa gttgacgaaa acatccgcga actgtgcgca ataaagaatt
      721 acggcaagga tcacaagtac atggactaca tcctctgccg caacaaggat atccgcaacg
      781 ccgaatggaa gacctgcgcc gtcaacggca tcgacccggc ggtcatggaa aagtgctcca
      841 ccggcgaaga aggcaagaag ctccattccg aaaacatcaa gatcgcgaag gccctggaaa
      901 tcggcgccag cccgacctgg cttgccaaca acaagttcaa gttctcgggt atcgccccgg
      961 aacagatcaa gaagcagttc tgcgaccaca acaaggaact gaagggctgc gcgaagacgc
     1021 tgtccggcga agccaaggtt cagcccggcg ccgctccggc cccgagctgc ggaggctagt
     1081 agcctgaccc caggatgacc gttgaaaggg gggccttcgg gtcccccttt ctttttccgg
     1141 caaccacttt tattcaagcc tgattgttga atttgtgcgg ctcaggggct aaatttgccc
     1201 cggagcgtaa cagggtgaac ccagatgaag acgatattcg aacctttccg gatcaagatg
     1261 gtggaaccga tcaggatgac gaccagggaa gaaaggctgg ccgcgataga gcaggccgga
     1321 tacaacccgt ttcttatccc ggcggaaaag gtgctgattg atctgctgac tgattcgggc
     1381 acgtcggcca tgagcaccga ccagtgggcc gcgatgatca agggcgacga ggcgtatgct
     1441 tgcgcccgaa gctggtatgc cttcgagtcg gcggtcaagg acatcaccgg cttcgcgcac
     1501 gtcataccga cacatcaggg ccgtgccgcg gaaaggctgc tcttttccgt gattgcgaag
     1561 gaaggaatgg ttgttccgtc aaacacgcat ttcgatacca ccagggcaaa tatcgagcac
     1621 aggggttgcc gggcgctcga cttcgtcgtg cctggcggcg aggatcccga ggtcatcgcg
     1681 gacttcaagg gcaacatgga catcgcccgc cttgaggaat gcattaagac gaacggcacg
     1741 gaaaagatac cggtcgtgat gctgacggtc acgaacaacg cgaccggcgg ccagcccgta
     1801 tcgatggaaa acatccgcca gaccagggcc gtgtgcgaca ggtacggtct tccattgttc
     1861 ctggacgtgg cccgctttgc ggagaacgcg tggttcatca agatgcgcga gccagggttc
     1921 gcggatcgca gcctgaagtc aatctgccag gagatgttct cttacgccga cggatgcacg
     1981 atgtcggcca agaaggacgc cctggtcaac atcggcggct tcatcgcctt aaatgatgaa
     2041 aaactggcgg ttggcctgaa gaacatgctg atcctgaccg aagggtatgc gacttacggc
     2101 gggctggccg gccgggatct cgaggcgatc gcccagggat tgaaagaagt cgtcgatgaa
     2161 gattacatgc gttaccggat tcataccgtg gcgtcgcttg ccgaaagatt gaaccaggcg
     2221 aacatcccga tattcaaccc gccgggtggc catgcctcta tcttgatgcc aggcggttcc
     2281 tgccgcatct tccgcccgaa cagttccccg gccaggccct tgttgccgaa ctgttcatcg
     2341 agggtggaat cagggctgtc gagatcggat ccgtgatgtt cggatactgg gacgcggacg
     2401 ggcgctttgt cggcgccccg aatgaactgg tcaggctggc tcttccgcga agggtatata
     2461 cgtccagcca catggattac ctggttgaag tcgttgtcga actgcatgcg cgccgtcatc
     2521 aggttcgcgg attgaagttc aggtacgagg cgccggtgct tcgccatttc acatcgacct
     2581 tcgaccgggt ctaggcaatt atcggggggc atttccgtgc aactgaagga tgcggtgctg
     2641 gtcggcctgg ataccgagac gaccgggctc gacaccaggc gggacagggt tttcgagatt
     2701 ggcctggtga cgtacagaag cggtgatgtc gttgaaaact ggggacgttt gatggatccg
     2761 ctggtcgaac ttgaccagaa ggtcgtggag acgaccggcg tcaccaccga agaagtgtcg
     2821 ggccagcctg tgtttgccga ggtggttgac gagatcgaga agaggatttc aggccagata
     2881 ctggttggat acaacatcat cgacttcgac ctgccgatgc tggcaaccga attcgaacgc
     2941 ctcgggcggc agatgccacc gtttcaggtg atcgatgtgc tggtcttcgc gcggcagctt
     3001 gcaaggggcg ggcgacaccg cctgggggac atggccgcaa ggttcggcgt ggaaatggac
     3061 accgcgcacc gggccaccgc cgatgccgac gccacggtca ggctcctgta cgccctggcg
     3121 ccaagtcttc cggacgacgt cgaaaagctt ctggtgcttc agtcccagtg gtccgcccag
     3181 cagcagtcca aaagggcgat gtggcgtgac cgcggggacg gaggtgccct gattggctcc
     3241 ggccaggccg cagccgtcgc gcacgatgac aaggttgccc tgggccccgc ctatctttac
     3301 ggtgacgaac gggatcccct taaggcattc atcaatcagt acgccgacca gaccgttcgt
     3361 cgctgaacct tcttgacctt tgttgccagt gcccgttacg atttcccgga atcgttaaac
     3421 cggtgcgttc cgttatgtcg tcaaggctgc cattcctgtc ccggttggct tcgctggccg
     3481 tggccttgtg cgcggcaggt tgcttttccg aaagcccgct gccggaatgc gacacgctgg
     3541 cggattgtcc ggtcaatcag gggtacaacg cctgtatcga cgggtggtgc tatcggacgc
     3601 aggcatgtgc cgacaggccg gtcgttgccg gtgacggctg ctgtgccctg accgagggcg
     3661 acagatccga agataccgac tgcatgctgg cggatcttgc cctttcctgt cgcgaccccg
     3721 ccggccccag cgttgacgat gacgggaaca tctatgttac gtgcctgatg cccttgaccg
     3781 acggaacccg gaatgtcgtg ctcaggcgcc ttgatacttc aggcaatctg tcggtgccgc
     3841 ttgttgccgg catcgggacg gtcgcgtttt cgccggtgat gggcaaggga agtacggtga
     3901 tggttcagtt cggcgccggt ttggcgcggt tcagcaccgt caatcttggc gatgccggcg
     3961 gccagtcaac cgggccggcc gcggcgcggg tggcgtccaa cggcggttcc gacgctttca
     4021 ggggaatcgg tggctggccg acgctggacg gcaaggtggt gctaatcgac gaggaaaaga
     4081 acatcc
//
LOCUS       DAOPPA010000295         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23816_length_4078_cov_4.058663, whole genome shotgun sequence.
ACCESSION   DAOPPA010000295 DAOPPA010000000
VERSION     DAOPPA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23816_length_4078_cov_4.058663"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKH54_09370"
     CDS             <1..252
                     /locus_tag="PKH54_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOD00146.1"
                     /translation="LGGEVENPKWKANFHGKKVYGDDGRVVFFVKKGLAGDDPHAVDG
                     ISGATITSRGVTWMLEFWMGKDGYGPYLAALKAAAGGAE"
     gene            255..924
                     /locus_tag="PKH54_09375"
                     /pseudo
     CDS             255..924
                     /locus_tag="PKH54_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
     gene            878..1495
                     /gene="nqrE"
                     /locus_tag="PKH54_09380"
     CDS             878..1495
                     /gene="nqrE"
                     /locus_tag="PKH54_09380"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOD00147.1"
                     /translation="MMEHYLSILFKSVFVENMALAFFLGMCSFLAVSRKVETAIGLGA
                     AVIMVMAIATPLNAFLYLWVLRDGALSWAHSSLAAVDLNFLSFLTFIATIAALVQIVE
                     MIIEKYSPKLYTRLGVFLPLIAVNCAILGASLFLVERNMTVGESAVFGAGSGLGWALA
                     IVALAAIREKMRYSDVPVGLRGLGIAFIITGLMAIGFMSFSGVSL"
     gene            1547..2773
                     /gene="nqrF"
                     /locus_tag="PKH54_09385"
     CDS             1547..2773
                     /gene="nqrF"
                     /locus_tag="PKH54_09385"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOD00148.1"
                     /translation="MALVITLGVAVFVLVIVALVAALSAIRSRLVSQRKVNIDINDDP
                     ENRIEVEAGGTLLGTLISNGIFLPSACGGKGTCGTCKCKVVSGGGSLLPTEASLITRK
                     EAREGVRLGCQVKVRDDIAIELPAEVFAVKQWKCKVRSNRNVATFIKELILELPEGEA
                     VPFRAGGYIQIECPPHVVKYSDFEIAAEYRGDWDKYNMWRYVSKVTETVTRGYSMANY
                     PGEHGIIMLNVRIATPPPDKPKAPPGMMSSYIFNLKAGDEVIISGPYGEFFATDTKAE
                     MCFIGGGAGMAPMRSHIFDQFLRLHTDRKVTFWYGARSLRESFYNEDFDRIQAENPNF
                     KWHLALSEPLPEDNWTGYRGFIHKVLLDEYLSKHPAPEEIEYYLCGPPQMLAACTRML
                     DSLGVEPDNIRYDDFG"
     gene            2853..3053
                     /locus_tag="PKH54_09390"
     CDS             2853..3053
                     /locus_tag="PKH54_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00149.1"
                     /translation="MTLLLTVLAGVAIFAVAVLALSLKVLAGSRTPLKEHACDVMCRH
                     GGGHACTCRNDGTCDDYPEGHP"
     gene            3228..3770
                     /locus_tag="PKH54_09395"
     CDS             3228..3770
                     /locus_tag="PKH54_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00150.1"
                     /translation="MRRILALSFVMVAAFTGCPVGSGDGGENAKISELMDYCPQMCLK
                     FQECNSALFALKWNSLADCEKECDPRTEANTCCVECDNRYAAGSAEHQDCFNVCRRDV
                     DVEACKTMCDGISDEYYHNACITGCNKEFSQACADVHAGIHECYLGLECERAIIYHDF
                     GGSASDLGECTNDDAVGTNC"
     gene            complement(3888..>4078)
                     /locus_tag="PKH54_09400"
     CDS             complement(3888..>4078)
                     /locus_tag="PKH54_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD00151.1"
                     /translation="SPVFNKKLNLVGLIFDGNIQSLGGAFWFDESVNRAVAVSSAAIM
                     ESLKKVYKADRLVKELSR"
BASE COUNT          804 a         1071 c         1263 g          940 t
ORIGIN      
        1 ctgggtggcg aggtcgaaaa cccgaagtgg aaggccaact tccatggcaa gaaagtatat
       61 ggggacgacg gcagagtggt cttctttgtg aagaagggcc tggccgggga cgaccctcac
      121 gcggttgacg ggatttccgg ggcgacaatc acatcaaggg gcgttacctg gatgcttgag
      181 ttctggatgg ggaaggacgg gtacggacct tacctggccg cgctgaaggc ggccgccggg
      241 ggggcggaat agtcatggct gacaatgcga tgaagacact gttgtcgccg gtattcgcga
      301 acaacccgat atcggtgcag attctgggca tctgcagtgc cctggccgtc accacgaaga
      361 tggatacggc gctggtcatg tcgatggccg tcatcgcggt caccgcgttt tccaaccttt
      421 cggtttcgct gattcgcagg tggattccgc cgcaggtgcg aatcatcatc cagttggttg
      481 taatcgcctc gctggttatc gtgaccgacc agatactgaa ggctttcgta ttcgacataa
      541 gtcgccagct ttcggttttc gtcggcctga tcatcacgaa ctgcatcgtc atgggacgcg
      601 ccgaggctta cgcgatgcag aacggcccgg tcgccagttt catcgacgga atcggtaacg
      661 gagtcggata cagcctggtg ctgcttattg tcgcgttctt ccgggaactc ctgggggccg
      721 gcaggttact tggcctgcag gttctgcccc tggcgagcga gggcggctgg tacaccccga
      781 acggcttgat ggtgctgtcg cccgcggcat tcttcattat cggtctgctg gtctgggcgc
      841 acaggacctg gaagccggaa ctgcaggaaa aggactgatg atggaacatt acctcagcat
      901 actgttcaag tcggtctttg ttgaaaacat ggccctggcg tttttcctgg gcatgtgcag
      961 ttttttggcg gtttcccgca aggtcgagac ggccatcggc cttggcgcgg cggtcatcat
     1021 ggttatggcg atcgcgacgc cgttgaacgc cttcctgtac ctgtgggtcc tgcgggacgg
     1081 ggcgctttcg tgggcgcact ccagcctggc ggccgtggat ctcaacttcc tgtcgttcct
     1141 gacttttatc gcgacgatcg cggcgctggt tcagatcgtc gaaatgataa tcgagaagta
     1201 ttctccgaag ctttacacca ggcttggcgt cttcctgccg ctgatcgcgg tcaactgcgc
     1261 gattctcggg gcttccctgt tcctggtcga gcggaatatg acggtcgggg aaagcgcggt
     1321 tttcggggcc ggatccggtt tgggctgggc attggctatc gtggcgctgg ccgccattcg
     1381 cgaaaagatg cggtacagcg acgttcctgt cggattacgg ggcctgggaa tcgcgttcat
     1441 cattaccggg ctgatggcga ttggtttcat gtcgttctcg ggcgtgtcgc tctaggacgc
     1501 ttttcgggga cctggccggc tggttccaac tgattggggc tgactgatgg cgttggtaat
     1561 cacactcggc gttgcggtct ttgttctggt catcgtggcc cttgtcgcgg ccctttcggc
     1621 catcagatcc aggctggtaa gccagcgcaa ggtcaatatc gacatcaatg acgacccgga
     1681 aaaccggatc gaggttgagg ccggcggaac cctgcttggg acactgattt cgaacgggat
     1741 attcctgccg tcggcgtgcg gtggaaaggg gacgtgcggg acgtgcaagt gcaaggtcgt
     1801 gtccggcggg ggaagcctgc tgccgaccga ggccagcctg atcacccgga aggaggctcg
     1861 cgagggcgtc cggcttggtt gtcaggtgaa ggtgcgtgac gacatcgcga ttgaacttcc
     1921 agcagaggtt ttcgcggtca agcagtggaa gtgcaaggtt cgttcgaacc ggaatgtcgc
     1981 gacattcatc aaggaactga tccttgaact gcctgaggga gaggccgttc ccttcagggc
     2041 gggcggatac atccagatcg agtgccctcc gcacgtcgtc aagtacagtg attttgagat
     2101 tgctgccgag taccggggcg actgggacaa gtacaacatg tggcgttacg tttcgaaggt
     2161 taccgagacc gtgacgcgtg gctattcgat ggccaactat cccggcgagc acggcatcat
     2221 catgctgaac gtgcgaatcg ccacacctcc gccggacaag ccgaaggcac cccccggcat
     2281 gatgtcgtcg tatatcttca acctgaaggc tggtgacgag gtcatcattt ccggccctta
     2341 cggcgaattc ttcgcgactg acacgaaggc cgagatgtgc ttcatcggtg gcggcgccgg
     2401 catggccccg atgcggtcgc acatctttga ccagttcctg cggctgcaca cggatcgtaa
     2461 agtgacgttc tggtacggcg cccgcagcct tcgggagtcg ttttacaacg aggatttcga
     2521 ccggatccag gccgaaaatc ccaatttcaa gtggcacctg gccttgtccg aaccgcttcc
     2581 agaggacaat tggaccgggt atcgagggtt catccacaag gtattgctgg atgagtacct
     2641 gtcgaagcat ccggcgccgg aagagatcga atattacctg tgcggtccgc cacagatgct
     2701 ggccgcctgc acaagaatgc tggactctct gggggtcgag ccggacaata tccggtacga
     2761 tgatttcggc tagggttttc accgatcgag tcacgcgacc tgtgaggccc gattgcatcc
     2821 gcccccgggg gcaatttttg taatggtttg aaatgacgtt gcttctgaca gttctggctg
     2881 gagtcgctat cttcgccgtc gctgttctgg ccctgtccct gaaagtcctg gcgggaagcc
     2941 gaaccccgtt gaaggaacat gcctgcgatg tgatgtgccg tcatgggggc ggccacgcct
     3001 gcacatgcag gaacgacggc acctgcgacg attatcccga aggtcatccc tgatcaaagg
     3061 cctcataccc ttgtacgggc ggatttaatc ttcattcagg aagcgtcttt acaaattatt
     3121 gacaaattcg atttacaggc acagtgttgg cgcaatctga cagaggattg acggaatcat
     3181 ggtggttccg tgcagtcttt tccccgtcag gcttatgggg gttccgaatg cgtcgaattc
     3241 ttgcgctttc ctttgtcatg gttgccgcgt tcactggttg tccggtcggc tcaggcgatg
     3301 gcggtgaaaa tgccaagatt tcggaactga tggactattg tccgcagatg tgtttgaagt
     3361 tccaggaatg taactcggcg cttttcgctc tgaagtggaa ttcgctggcg gattgtgaaa
     3421 aggaatgcga tccccgtacc gaagccaata cctgctgtgt cgagtgtgac aaccggtacg
     3481 ctgccggctc ggctgaacat caggactgct tcaacgtgtg tcgtcgcgac gtcgatgtcg
     3541 aagcatgtaa gaccatgtgt gacggcattt ctgacgagta ctatcacaac gcatgcataa
     3601 ctggctgcaa caaggaattc agtcaggcct gtgccgatgt ccatgccgga atccatgagt
     3661 gttatcttgg tcttgaatgt gaaagggcga ttatctatca cgacttcggc ggcagcgcat
     3721 ccgacctggg cgaatgtacc aacgatgatg ctgttggcac caactgctga taacccaaca
     3781 tgagcagtca gaacgtaatg attggctcgt gacgcaccct ggcatgggcg aaatgccttt
     3841 caaccggcta aggtcaggaa atcagtccgg aatggggcgt tgtggcgtca gcgggaaagc
     3901 tccttgacca ggcgatctgc cttataaacc ttcttaaggg attccatgat tgcggcggac
     3961 gataccgcca cggcgcggtt aacggactcg tcgaaccaga atgcgccgcc cagagactga
     4021 atattgccgt caaagatcag gccgacaagg ttcagtttct tgttgaagac cgggctgc
//
LOCUS       DAOPPA010000296         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23835_length_4076_cov_2.923153, whole genome shotgun sequence.
ACCESSION   DAOPPA010000296 DAOPPA010000000
VERSION     DAOPPA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23835_length_4076_cov_2.923153"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1204
                     /locus_tag="PKH54_09405"
     CDS             158..1204
                     /locus_tag="PKH54_09405"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOD00152.1"
                     /translation="MGRVVEEAPDCLVDGGKIVSGVFRTPVRDVNILDAMAAEGTVSR
                     AFWKMRMKEWCGIGFVHPDWYLSVMIQDAKYVSSGMVYLWDRNRLKYFRHGWSAAGTA
                     DSLAANQYDGACSAKRRGFSLEFEHRLDRGLHNIRIDVAATRSAPAVKAELRLHEDLS
                     TVTPLVASLHIDSRHHAYTHKAPMGIEGWMTVGDTRVIFEPDRDTANMDEHKAIYPYR
                     THWLWGSFAGRDSQGRLFGVNLADHVFADQETNNENCVWLGGDIRLPGRIEYQMDPSR
                     PFDVWRIDDRTGTVDLQFHPAGRFVQKANLLVAGIDYYQMFGSWRGVVQGPDGMMHDV
                     PGLFGVAERMDTRF"
     gene            complement(1259..3310)
                     /locus_tag="PKH54_09410"
     CDS             complement(1259..3310)
                     /locus_tag="PKH54_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00153.1"
                     /translation="MFTSIIRNGAFLVAPILALTIAAGCGGDDPAQDVTDGQDVIQDS
                     GSIDDVIQTDPGRDPGSPELPGDSIGDLGTDLTDDVPAELPTDNGGDIPVDAGNDVPA
                     DEGVDTVDADATERPVEPCIMTKRLDLNCDGTPDTCQVTEHDANGHRKVVMIDAGCDG
                     KPPFSDCANTTYSEDGLLMSRSESSQCGETPDSACATFHYDEFGRKATETLDEECIGT
                     PGECYTYTYDPSGNLRAVEMDLGCADEMLACLEKTFDAKGNVLTSKSDVGCDDNPSND
                     SNCTEFGYYANGKPKDVSLDYACVDSPTLCHRYDYFTISGRGTVVVEEQNNCEGGQRI
                     CKNSVFKGGYQYLTESEVDQGCDGTPSADCRKYSRDGNGNVTREEVDSNCDGTPEYCI
                     FSRYSGEGVLIFQSKSMTCNQQPIDCVTFSYDGDCRRTIRTEMEADFDISCGQAVGGN
                     TANSGHAVMSKYNCIGWTPTAKEQIFRYVTPAEGCEYKWKVTAAMTSLSADLDLLLLS
                     ELNPDTCTAKSAAAGMADESLSWQAIPGDDFFLVVEGYGPTTGTYTLSVTCECLAEPL
                     PSCVDNCGGRGQDGTCGCDPTCQWIGDCCTDWCQACAEDCEASRCGEVDGTGYCDGNT
                     AKRCVNGVLVTEPCGDKTCDIVGYGETWSPNPCSFLPAGIPCDANVEATYLFEKAECV
                     E"
     gene            complement(3591..3890)
                     /locus_tag="PKH54_09415"
     CDS             complement(3591..3890)
                     /locus_tag="PKH54_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOD00154.1"
                     /translation="MRPSNRIPTHPGVILATDFLKPLGITQMAFAAHLGIPIQRLNEL
                     IRGKRGVTPDTAWLLSQALGTTPDFWMNLQAAHDLAACRPVRKIKPLHAVRDDAG"
BASE COUNT          798 a         1170 c         1220 g          888 t
ORIGIN      
        1 cgaactagtc agatctttga ctattttatt gtaatttgtc aagcgacggg ctgtctgagc
       61 catccccgca tggttacggg cgggatgcgg cgaccgacct gttgcggtca cgagcggtcg
      121 cggtttatga tgcgccatca ggtcacgggg aaggttcatg ggcagggttg tcgaggaagc
      181 tccagattgt ctggtagatg gcggaaaaat cgtttccggg gtgttcagga cgcctgtgcg
      241 ggatgtaaac atccttgacg ccatggctgc cgaaggcacg gtctcgcgag ccttctggaa
      301 gatgcgcatg aaggaatggt gcggaatcgg tttcgtgcac ccggactggt acctttcagt
      361 gatgatccag gatgccaagt atgtctcttc gggaatggtc tatctgtggg acaggaaccg
      421 gctgaagtat ttccggcacg gctggtcggc cgcgggaacg gcggacagtc ttgccgcgaa
      481 ccagtacgac ggggcgtgca gcgcgaaaag gcgcgggttc agcctggaat tcgaacaccg
      541 gcttgaccgg ggactgcata acatccgcat cgatgtggcg gcgacgcggt cggcaccggc
      601 cgtgaaggcg gaactgaggc ttcacgagga cctttccacg gtcaccccgc ttgtcgcaag
      661 ccttcacatc gattccaggc atcacgctta cacgcacaag gccccgatgg gcattgaggg
      721 ctggatgacg gtgggggaca ccagggtgat cttcgagcct gaccgggaca ccgccaacat
      781 ggacgagcac aaggcgattt atccctatcg cacacactgg ctgtggggct cgttcgccgg
      841 tcgtgacagt caggggcggc ttttcggggt caacctggcc gaccacgtct tcgccgacca
      901 ggaaacgaac aacgaaaact gcgtctggct gggcggtgac atccgactgc ccggtcgaat
      961 cgaatatcaa atggatcctt cgcgcccctt cgacgtgtgg cggatcgatg atcggacggg
     1021 aaccgtcgat ttgcagttcc acccggccgg caggtttgtc cagaaggcca atctgctggt
     1081 tgccggcata gattattatc agatgttcgg aagctggcgc ggcgtggtgc aggggccgga
     1141 cggcatgatg catgacgtgc ccggactgtt cggggtcgcg gaacgcatgg acaccaggtt
     1201 ctgatcagac agcaagcagc gcatcaattg agggaacgga ccattttccg gccgtggttc
     1261 attcgacgca ttcggccttt tcaaacagat aggtcgcttc aacattcgcg tcgcatggaa
     1321 tgccggccgg caggaacgag caggggttcg gggaccaggt ctcgccatat cccacgatat
     1381 cgcaggtctt gtcgccgcag ggttcggtaa ccagcacgcc gttgacgcaa cgcttcgccg
     1441 tgtttccatc gcagtatccg gtgccatcga cttccccgca ccttgatgcc tcgcagtcct
     1501 cggcgcacgc ctggcaccag tcggtgcagc agtcaccaat ccactggcag gtcgggtcgc
     1561 agccgcaggt tccgtcctgt ccccgcccac cgcaattatc gacgcaggac ggcaggggtt
     1621 cggccaggca ctcgcatgtg accgacaatg tataggttcc tgtcgttggc ccgtaccctt
     1681 cgaccacaag gaagaagtcg tcaccaggta tcgcctgcca cgacagagat tcgtcggcca
     1741 ttcccgcggc ggcggacttc gccgtgcacg tgtccgggtt cagttccgac agcagcagca
     1801 gatccagatc cgcggaaagc gaagtcattg cggcagtcac cttccatttg tattcacaac
     1861 cctcggcagg agtgacatac ctgaaaatct gctcctttgc ggtcggcgtc catccgatgc
     1921 agttgtattt cgacatgacg gcgtggccgc tgttggccgt gtttccaccc acggcctgtc
     1981 cgcacgagat gtcaaagtca gcttccattt ccgtccggat ggttctgcgg cagtccccgt
     2041 cgtagctgaa ggtcacgcaa tcgatcggct gctggttgca agtcatcgat tttgattgaa
     2101 aaatcaggac gccttcaccg gaatagcggc tgaaaatgca gtattccggg gttccgtcgc
     2161 agttcgagtc cacttcttcg cgagtgacgt tgccgtttcc gtcgcggctg tacttcctgc
     2221 agtccgcgct tggcgtgccg tcgcagccct gatccacctc cgattcggtc aggtactggt
     2281 agccgccctt gaaaacgctg tttttgcaga tgcgctggcc accttcgcaa ttattctgct
     2341 cctcgaccac gacggttccg cgtccgctga tcgtgaaata gtcgtaccgg tggcagaggg
     2401 tcggcgaatc cacgcacgcg taatccaggg acacgtcctt cggcttgccg ttggcgtaat
     2461 agccgaattc ggtgcagttg ctgtcgtttg acggattgtc gtcgcacccg acgtcgctct
     2521 tgctggtcag cacgtttccc tttgcgtcga aagttttttc aaggcacgcc agcatctcgt
     2581 cggcacaccc taagtccatc tcaacggctc gaagatttcc gcttgggtca tatgtataag
     2641 tgtagcattc acctggtgtt ccaatgcatt cctcgtcaag tgtctcggtg gcctttcgcc
     2701 cgaattcgtc gtaatggaac gtcgcgcagg cagaatcggg cgtttcgccg cactgtgagg
     2761 attcggagcg cgacatcagc aggccgtctt cagaataggt ggtgttggcg cagtccgaaa
     2821 aaggcggctt gccgtcgcag cccgcatcaa tcatcacgac cttccggtgt ccgttggcat
     2881 cgtgttcagt gacctggcag gtgtccggcg tgccgtcgca gttcaggtcc agccgtttcg
     2941 tcatgatgca cggttccact ggacgctcgg tggcatccgc atcaaccgtg tccacgcctt
     3001 catccgccgg cacgtcgttt ccggcgtcaa ccggaatgtc cccgccgttg tccgtcggca
     3061 gttcagccgg aacatcatcg gtcaaatccg ttccgagatc gccaatcgag tccccgggca
     3121 actcgggcga gcccgggtca cgaccgggat ccgtctggat tacgtcatcg atggatcccg
     3181 aatcctggat cacgtcctgc ccgtccgtca cgtcctgagc cgggtcatcg ccaccgcacc
     3241 ccgcagcgat cgtcagtgcg agaatcggcg caaccagaaa ggcaccattg cgaataatgc
     3301 ttgtaaacac ttcagtttcc ccctgaatct ggaatgaaat atgcttgatc taatgctcac
     3361 caatggtcag gcatattgcc gctgaatgtc aagctgtgtg cctgccgatg atccccttat
     3421 ggagagataa ttgtttttgg tggccgcgtc gagactggag taggtcagga ttgacataat
     3481 ggactgtgca gaggtcagta tgagtttcca gcttccgata gcatgagtgg acgggccagg
     3541 cgtggggcgc ctgtccatgc ccaaggtgac catgcgggaa ggtgcggcag ttacccggca
     3601 tcatccctga cagcgtgcag cggtttgatt tttctgactg gacggcaggc ggccaggtcg
     3661 tgggcggcct gaagattcat ccagaaatcc ggggttgtgc cgagggcttg cgagagcagc
     3721 caggcggtat cgggggtaac gccacgcttg ccgcggatca gctcgttcaa ccgctgaatc
     3781 gggatgccca gatgggccgc gaacgccatc tgcgtaatgc caagcggctt cagaaagtcg
     3841 gtcgcgagaa tcacacccgg atgtgtcgga atcctgtttg aaggtctcat agaccacccc
     3901 ggcggaacat tcaagattaa ccgtcttggt gaaccatcaa aaagagcctt tgtcgtcttg
     3961 tccccgaatg atgaaatcac cgttcagccc cagggcatgg agaatattaa cggttacacg
     4021 gtatccatgc aatagtagag tcgcgcacag aggcttatat cggcggccgg atcgga
//
LOCUS       DAOPPA010000297         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_23989_length_4055_cov_3.376750, whole genome shotgun sequence.
ACCESSION   DAOPPA010000297 DAOPPA010000000
VERSION     DAOPPA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23989_length_4055_cov_3.376750"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKH54_09420"
     CDS             <1..804
                     /locus_tag="PKH54_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00155.1"
                     /translation="GWGNSCVNGRCRWVSAEYTVTCNSNGMCLVPEGSFWMGCNEQAF
                     DDCISDEVPFHEVELLQFFIDRTEVTQGQYKKCVDDGSCTKPSCNWNPGLTPNQPVVC
                     VTWPQAGKYCSWANKRLPTEAEWEKAAKGIDGRIFPWGIVAPTCEYAVLDNGSPGCGT
                     GAPKDVCGTSPKGDSPYGLCDMAGNAAEWVSDWYGPSYYAGSGDSNPFGPEEGTLRVR
                     RGGSYVDTPEYLAVRRRGVDSPLMGYSYLGFRCVADDNVIPGEFGFKSR"
     gene            832..2625
                     /locus_tag="PKH54_09425"
     CDS             832..2625
                     /locus_tag="PKH54_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00156.1"
                     /translation="MKTVLFLRREFDRILMNSTRTAVACIVAVALAASVASCGGGGDE
                     VNCIDSMDCAFGQACRDAECVVVPCASAEGCVPKVEICAAPVLVLDGIVRSFCTAPCN
                     VFHECPSGQVCRDKICQVSGGIDQDVLGDAGEDAVGDVIDAVGEDAPSPEDTIWQDGS
                     DDPGTGDSGSQDTYDSISCGSDCGNYVDIDDPDTNIPEDLNGQDTNGQDTSGQDTSVV
                     DNGALTDVSAPPICPANQDIANLLRQAEGTVDVMLCGAVVTKVTQKGWYIADDSSTRG
                     MYIYEGSNPDWGYHVEVGDYVRMHVSSYSNYLGTQQVYGKENVTVHGKADYREYVVDL
                     STGTVPSESIESRIVRIEGVKVTAAAGKSGTIDYGTARNVSVLVEYNDIPCIGLQADI
                     VAGIISQYNSVFQVVLTEDADLDNVSLANCETPPEYDMSNWDFEKQSVEEDPPEGFTK
                     GPGNTFTCVRDVDGGTDGSAGIVLTWTSTDMQDLIQGYRFPTAAGSTVIFSVDVLDND
                     PAGRVRLGLLFYDADNNFITGSNSWSSDYSMNSPVWQSLSHQAVAPAGAAFAEGKLRM
                     YDHQPPDGTWGNPNASAFVTIDNWEMTSVAP"
     gene            complement(2719..3051)
                     /locus_tag="PKH54_09430"
     CDS             complement(2719..3051)
                     /locus_tag="PKH54_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00157.1"
                     /translation="MFYRHLSISALLLFSVLFCGCGGISLGQTRITYHHSNKEKDSFW
                     MTVSKGSVDKNDITTSLWYCQSRDKAVCSQVQLTKCAKAEDCQMESSFITQDFIETPA
                     IGGYVQVY"
     gene            complement(3150..3743)
                     /locus_tag="PKH54_09435"
     CDS             complement(3150..3743)
                     /locus_tag="PKH54_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00158.1"
                     /translation="MNKAIQGSGAFRMYAAIAMVFLAISITACEKPAPASLADYPGFW
                     VSGGDTAIFVSSDGLTVHLSNLKVIDSFNRTEGDPPLSCEKTFQYAPGCGKGVCLAII
                     QGSAVDGSDGHVVFEGELTQEQSPDFDLVTGILFEPDQPDGTPTLKLSLSPRSAGCLL
                     TDQAYFLMTTLTWAPDSTHCPAAPECLPEPAQPDEVP"
BASE COUNT          851 a         1031 c         1263 g          910 t
ORIGIN      
        1 ggctggggca attcttgtgt caatggccgc tgcaggtggg tatccgcgga atacacagtc
       61 acatgcaatt ccaatggaat gtgtcttgtt cccgaaggtt cattctggat ggggtgcaat
      121 gaacaggcct tcgacgattg catttcggac gaagtgcctt ttcacgaagt agagttgtta
      181 cagtttttca tcgacaggac cgaagtcaca cagggtcaat acaagaagtg cgtcgatgac
      241 gggagttgca cgaagccgtc atgcaactgg aatccgggct tgacgccaaa tcagccggtt
      301 gtctgtgtca cgtggccgca ggccggcaag tactgctcct gggcgaacaa acgtctgcca
      361 accgaggccg agtgggaaaa ggcggcaaag ggaatcgatg gccggatttt tccctggggg
      421 atcgttgcgc cgacatgtga atacgcggta ttggacaacg gctcccccgg gtgcgggact
      481 ggtgcaccaa aggatgtgtg cggcacctcg ccgaagggcg attcccctta tggcctgtgt
      541 gacatggccg gcaatgccgc ggaatgggta tcggattggt acggcccgag ttattacgct
      601 ggaagtggcg actcgaatcc cttcggcccg gaggaaggta cgcttcgtgt caggcgcggg
      661 ggaagctatg tggatacacc ggaatatctg gccgtccgtc gccgcggcgt cgatagtccg
      721 ttgatgggct attcctatct gggtttccgg tgtgttgccg atgataacgt tattccgggt
      781 gaattcggct tcaagtcgcg ctgattgcct gcgtggcgaa cggggttttc gatgaaaaca
      841 gtgcttttct taaggcggga atttgatcga atcctcatga acagcacacg gactgcggtt
      901 gcgtgcattg tggccgtggc gctggccgca tccgttgctt catgcggtgg cggtggtgac
      961 gaggttaact gcatcgattc gatggattgt gctttcggac aggcatgcag ggatgcggag
     1021 tgtgttgtcg tgccgtgcgc ctcggccgaa gggtgcgtcc cgaaagtgga gatttgcgcc
     1081 gcgccggtcc tggtactgga tggaattgtt cggtccttct gcacggcgcc atgcaatgtg
     1141 ttccacgagt gtccgtcagg ccaggtctgt cgggataaga tttgccaggt gtcaggcggg
     1201 atcgatcaag atgttttggg ggacgcaggt gaagatgccg ttggtgatgt aattgatgcg
     1261 gttggcgaag acgctccgtc acctgaggat acgatctggc aggatgggag tgatgacccc
     1321 gggacgggtg attcaggttc ccaggacact tacgattcga tctcttgtgg ttccgactgc
     1381 gggaattatg tcgatattga tgatccggac acgaacatcc cggaagactt gaacggccag
     1441 gacaccaatg gccaggatac cagcggccag gacaccagcg tggtcgataa cggcgcgctc
     1501 accgatgtaa gcgccccgcc gatttgcccg gccaaccagg acatagcgaa cctgttaagg
     1561 caggccgagg gcactgttga cgtcatgctt tgcggcgctg tcgtcaccaa ggtcacccag
     1621 aagggctggt acatcgcgga cgacagttcg acacgcggca tgtacatcta tgaaggatcc
     1681 aatccggact ggggttacca tgtcgaagtc ggcgactatg tccggatgca cgtgtcctcc
     1741 tattccaatt atctgggaac ccagcaggtt tacggaaagg aaaacgtgac cgtccatggt
     1801 aaggccgatt accgggaata tgtcgttgac ctgtcaacag gcaccgtccc cagtgaatcg
     1861 atcgaaagcc gcatcgtccg catcgaaggt gtgaaggtca ccgcggcggc cggcaagagc
     1921 ggaacaattg actacggcac cgcaaggaac gtcagcgtct tggtggaata caatgatatt
     1981 ccctgcatcg gtcttcaggc tgacatagtt gccgggatca tttctcagta caacagcgtg
     2041 ttccaggtgg ttctgaccga agacgccgat ctggacaacg tcagcctggc caactgcgaa
     2101 acaccccccg aatacgacat gtccaactgg gacttcgaaa agcagagtgt cgaggaagat
     2161 ccgccggagg gcttcaccaa ggggccgggc aacacgttca cgtgcgtccg tgacgtcgat
     2221 ggtgggaccg atggcagcgc tggcatcgtc cttacatgga catcgacgga tatgcaggac
     2281 ttgattcagg gatacaggtt cccgacggcc gccggttcaa ccgtgatttt ctcggttgac
     2341 gtcctggaca acgaccctgc gggcagggtt cgcctgggcc tgttgttcta tgatgccgac
     2401 aacaacttta ttaccggctc gaatagctgg tcttcggact attccatgaa ttcacctgtc
     2461 tggcagtcgc tttcccatca ggcggttgcg ccggccggcg cggcattcgc cgaaggcaag
     2521 ctacggatgt acgatcacca gccgcccgat ggcacatggg gcaacccgaa cgcttcggca
     2581 ttcgtaacca tcgacaattg ggaaatgact tccgtggctc catgacggca agatgggcag
     2641 ggagggttca accggattcg ttgtcccaga ccctgggtca attcctgcgg gctgggtgtg
     2701 gtttccgaac tgtggtcgtc agtagacctg gacgtagccg ccgattgctg gcgtttcaat
     2761 gaagtcctgg gttatgaacg atgattccat ctgacagtct tcggccttgg cgcacttcgt
     2821 cagttgaacc tgcgaacaga cggccttgtc cctgctctga cagtaccaga gcgaggttgt
     2881 gatgtcgttt ttatcaacgg aacccttcga gacggtcatc cagaaagaat ccttttcctt
     2941 gttcgaatgg tggtaggtaa tcctggtctg gcccaatgaa attccgccgc aaccgcagaa
     3001 gagaaccgag aaaagaagaa gggcggaaat ggaaaggtgt cggtaaaaca tgaaatcacc
     3061 gtttgaagtt ccagcgacaa gtaccgcatt tgcggcagat ggtcaatcgt tggaaggctt
     3121 gaatctggat ggatttaaga tggtccaaat cagggcactt cgtctggctg ggccggttcg
     3181 ggcaggcatt ccggggcggc ggggcaatgg gtgctgtcgg gggcccacgt cagggtcgtc
     3241 atgaggaagt aagcctgatc cgtcagcagg cacccggccg accgcggtga aagagacaac
     3301 ttcaatgtcg gcgtgccgtc aggttggtcg ggctcgaaaa ggatcccggt tacaaggtcg
     3361 aagtccgggc tttgttcctg ggtcagttct ccttcgaata ccacatgacc gtcggaacca
     3421 tcgacggccg atccctggat gattgccagg cacactccct tgccgcatcc tggcgcgtac
     3481 tgaaacgtct tttcgcagga aagtggcggg tcgccttccg tcctgttgaa gctgtcaata
     3541 accttcaggt ttgaaaggtg cacggtcagg ccgtcggacg acacgaaaat ggcggtgtcg
     3601 ccgcctgaaa cccagaatcc cgggtagtcg gccagcgatg cgggcgcggg cttctcgcat
     3661 gcggtgatcg atattgcaag aaagaccatc gcgattgccg cgtacattct gaatgcccca
     3721 gatccctgaa tggctttgtt cattggtcat tcaccaattg gttgggccgc attaatcgag
     3781 tatcagccaa atgtcgtccg tgcgaaaggc caagttgaca accactgcat gtcggtctat
     3841 tttacggcca gtcagtaatt gaggttcggt tcatgaattc cgttcgagtc cttcggattg
     3901 gtgcattttt ggcggtgtct gtcttgttgt cggcgtgcgg tggcggcgtt caggtgacgg
     3961 atgcgattta tccggacgat gtgaatccac cggccgatgt ccatgaagtg gaaccgggcg
     4021 attgagttgc tgacgggcgt ttcggcgagc agcgt
//
LOCUS       DAOPPA010000298         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24133_length_4038_cov_3.740648, whole genome shotgun sequence.
ACCESSION   DAOPPA010000298 DAOPPA010000000
VERSION     DAOPPA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24133_length_4038_cov_3.740648"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKH54_09440"
     CDS             <1..622
                     /locus_tag="PKH54_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00159.1"
                     /translation="ANLLLLKREYPALKVHTSYVVNKRNYRLFREFFDVMLPFRVDQH
                     VFNVMMPEGRGREFFDNLVIPYSMVAAEFKKFTDALPPEGLNRVFILDIPYCTTVGIK
                     DSARGYVERYFHYEPDSTVLFQDGRTPDQRRPEADAEVYSRKVMSGDKHEYSKVTKTV
                     HDQMVRIKRPECEQCGYNHICRGVFTSYIDRFGWDEFVPVQRKGDK"
     gene            623..1567
                     /locus_tag="PKH54_09445"
     CDS             623..1567
                     /locus_tag="PKH54_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00160.1"
                     /translation="MADTPQTERIDIKTGWNCNNRCLFCVQGNKRAQYPTKDTAEVFK
                     LLEEGRRDADSIVFTGGEVTIRQDLMEIVAYAKSLGYRSIQIQTNGRMLAYKKICEDL
                     INAGATEFSPALHGHTAALHDYLVHAPGAFKQTVRAIANLKELGQYVLTNSVICRANY
                     RHLPELATLLTSLGVDQYQFAFVHPLGTAAELFTSIVPRMSLIEPFVHRGLDVGIKAG
                     RSVMTEAIPYCFLKGYEIFAGERVVPRTKILEGHVTIQDYTVHRLTEGKAKAPAAPSA
                     RLMTCVKARGTSIPTSTAGTNSNPAGTSRVPTRISFPA"
     gene            1592..2965
                     /locus_tag="PKH54_09450"
     CDS             1592..2965
                     /locus_tag="PKH54_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00161.1"
                     /translation="MGDFDARQAAIDEKRLWVRITRSCNNRCEFCLDSGALGGGPIPE
                     DEVRARLRRGRDEGYTRLILSGGEATTHPKFLDFVKYGTEIGYSWIQVISNGRMFAYE
                     SFARQAVENGLREATLSMHAHNQELFERLTGAGGAFKQAVRGLVNLKRLGTVVSVDIV
                     LTRLNLPYLREIMEFYISLGVMEFDLLHLIPFGRGFEENREELYADDAMIGRELARAL
                     ELDRIPGMFIWTNRLPIRYLEGYEHLFQDPHKLYDEVLGERQSFRRLFADGTPIECRQ
                     PERCAVCFLRDFCDAAIRYAAGQWDSKPVPPATELDGALARRIIADGSRDAAGDFPDG
                     LRIAVREHFGEARSVAPTVEEVREVTRITGLPVMGLPPCLGGVPSCQDAPDPGCVQRW
                     PAPPPGILDARGVPEVAGFVGFYIRELYRVKSLRCESCSMNARCAGLQVNLARQWGLK
                     VLTPVTE"
     gene            3086..4018
                     /locus_tag="PKH54_09455"
     CDS             3086..4018
                     /locus_tag="PKH54_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00162.1"
                     /translation="MANLGYIQVTRLCNQKCRMCSNPEREATLTFEQATALIDDFVSR
                     HYDGIILTGGEPTMHPQLGEIIGYAKARGMASRIITNGQKTCDLDFIRGLVENGLTHL
                     HVSIQSYDPKIQAFLSGKEDSLENAFKTLENVEKCGITADVNTTINHYNAAHLDKTVA
                     YLIRRFPFLHHFVWNNLDPSMNRATENPDVIHELWEMEVPLYRAMMLLHATGRTFRAE
                     KVPLCYMAEFAWASTESRKIIKGEERIVRFLDEKGMVRETAFRHGKAECCQHCTFDGI
                     CAGLFDMDVHYKSSELYPVFLDPEPIRQKVLSEP"
BASE COUNT          873 a         1201 c         1199 g          765 t
ORIGIN      
        1 ggccaacctg ctcctgctga aaagggaata tccagccctg aaggtccaca cgtcatacgt
       61 tgtaaacaag cgcaactaca ggcttttccg cgaattcttc gacgtgatgc ttccgtttcg
      121 cgtcgatcag cacgtgttca acgtgatgat gccggaaggt cgcggccggg aattctttga
      181 caacctggtc atcccgtaca gcatggttgc cgcagaattc aagaagttca cggatgccct
      241 gccgccagaa ggcctgaaca gggttttcat actggacata ccttactgca cgacggtcgg
      301 catcaaggat tcggcccgcg gatacgtgga acggtacttc cactacgaac cggacagcac
      361 ggttctgttc caggacgggc ggacgccgga ccagcgtcgt ccggaggccg atgccgaggt
      421 ctacagccgc aaggtcatgt ccggggacaa gcacgagtat tccaaagtca cgaagaccgt
      481 ccacgaccag atggtccgca tcaagcgccc cgaatgcgaa cagtgcggat acaaccacat
      541 ctgccggggc gtcttcacca gttacatcga tcgcttcgga tgggacgaat tcgttccggt
      601 ccagcgcaag ggggacaaat agatggccga cacgccgcag acggaacgca tcgacatcaa
      661 gaccggctgg aactgcaaca accgctgcct gttctgcgtc cagggaaaca agcgtgcaca
      721 gtaccccacc aaggacaccg ccgaagtctt caagctgctc gaggaaggcc ggcgcgatgc
      781 cgactccatc gtcttcaccg gtggcgaggt gacgatacgc caggatctga tggagattgt
      841 cgcgtacgcg aaatcgcttg gataccggtc gatccagatc cagaccaacg gcaggatgct
      901 ggcatacaag aagatctgcg aagacctgat aaacgccggg gcgaccgagt tctcaccggc
      961 gcttcacggg cacaccgccg cgctgcacga ttacctggtg cacgcgccgg gcgccttcaa
     1021 acaaaccgtt cgcgccatcg ccaacctgaa ggaacttggt cagtacgtcc ttacaaacag
     1081 cgtcatctgc cgggccaact accgccacct tcccgagctt gcgacccttc tgacgtcgct
     1141 gggggtcgat cagtaccagt tcgcgttcgt gcacccgctt ggaacggcgg ccgagctttt
     1201 cacttcgatc gttccgcgca tgtccctgat cgagccgttc gttcatcgcg gacttgacgt
     1261 cggcatcaag gcggggcgaa gcgtgatgac cgaggccatc ccttactgct tcctgaaggg
     1321 gtacgaaatt ttcgcgggcg aacgggtcgt tcccagaacc aagatcctgg aaggccacgt
     1381 aacgatacag gattacacgg tgcaccggct tacggaagga aaggccaagg cccccgctgc
     1441 gccgagtgcg cgtttaatga catgtgtgaa ggcccgtggc acgagtatcc cgacaagtac
     1501 ggctgggacg aattcgaacc ccgcagggac ttctcgggtt cccacccgga tctctttccc
     1561 ggcgtgaagc tttgaaatct ggtgacaggc agtgggcgac ttcgacgcaa gacaggcagc
     1621 aatcgacgaa aagcgtctct gggtaaggat cacccgatcc tgcaacaacc ggtgcgagtt
     1681 ctgtcttgat tccggggcgc tgggcggcgg gccgattccc gaggacgaag tccgcgcacg
     1741 gcttcggcgg ggccgcgacg agggatacac ccgcctgatc ctttccggcg gcgaggcgac
     1801 cacccatccg aagttcctgg acttcgtcaa gtacggcacg gagatcggat attcatggat
     1861 ccaggtcatc agcaacggcc ggatgttcgc ttacgagagc ttcgcgcggc aggcggttga
     1921 gaacgggctg cgcgaggcaa ccctttcgat gcacgcccac aaccaggaac tttttgaaag
     1981 gctcaccggg gccggcggcg ctttcaagca ggctgtccgg ggccttgtga acctgaagag
     2041 gcttggaacc gtcgtatccg tcgatatcgt cctgacgcgc cttaacctgc cataccttcg
     2101 cgaaatcatg gaattttaca tcagccttgg cgtgatggaa ttcgatctgc tgcacctgat
     2161 cccgttcggg cgcgggttcg aggaaaaccg cgaggaactt tacgccgacg acgcgatgat
     2221 cggacgcgaa ctggcgcgcg ccctggaact ggatcgaatc ccggggatgt tcatctggac
     2281 caacaggctg ccgattcgat acctggaagg ttacgagcat ctgttccagg atccgcacaa
     2341 gctttacgat gaagtgcttg gggaacgtca gtcctttcga aggcttttcg ccgacgggac
     2401 gccgatcgag tgccgccagc cggaacgatg cgctgtctgc ttcctgcgcg atttctgcga
     2461 cgcggcaatc aggtatgctg ccggacagtg ggattcgaag cccgttcccc cggcgaccga
     2521 gctggacggc gcgcttgccc gccgaatcat cgctgacggc agccgtgacg cggctggtga
     2581 cttcccggac ggattaagga ttgcagtaag ggaacacttc ggcgaggcaa ggtcggtggc
     2641 accgacggtt gaagaagtcc gggaagttac gcggattacg gggctgccgg taatgggcct
     2701 gccgccctgc cttgggggcg tgccgtcctg ccaggatgcg cccgacccgg ggtgtgtaca
     2761 gcgctggcca gcgccgcccc cgggcatact tgacgcccgc ggcgttccgg aagttgccgg
     2821 gttcgttggc ttttacattc gggaactgta cagggtgaaa agcctgcgat gcgaatcgtg
     2881 ctcgatgaac gcgcggtgcg ccggattgca ggtgaacctg gcgcgccaat ggggcctgaa
     2941 ggtcctgacg cccgttacgg agtgagtcgc gggtgatcgc cattcgcccg aaggccgcgc
     3001 cggacgcgtc cgcggtattg tgccgaccca tttacccgat cggcgcgggc attcgcccaa
     3061 ccgatcttga ggtgccgata acgttatggc caaccttgga tacatacagg taacacgact
     3121 ctgcaaccag aaatgtcgca tgtgttcgaa cccggagcgc gaggcgaccc tgacgttcga
     3181 gcaggccacc gcccttatcg atgacttcgt cagccgtcac tatgacggga tcatccttac
     3241 ggggggcgag ccgacaatgc atccgcagct tggcgaaatc atcggttacg ccaaggcccg
     3301 cggcatggcg tcgcgcataa tcacgaacgg ccagaaaacc tgcgacctgg atttcattcg
     3361 cggtctggtc gaaaacggcc tgacacacct ccacgtctcg attcaatctt acgacccgaa
     3421 gattcaggca ttcctttccg gcaaggaaga cagtctggaa aacgccttca aaacgcttga
     3481 aaatgttgaa aaatgcggaa tcactgccga cgtcaacacc accataaacc attacaatgc
     3541 ggcgcacctg gacaagaccg tcgcatacct gatccgcagg ttccccttcc tgcaccactt
     3601 cgtctggaac aaccttgatc cctcgatgaa ccgggccacg gaaaacccgg atgtaattca
     3661 cgaactgtgg gaaatggaag tgcccctgta ccgcgcgatg atgcttctgc acgcgaccgg
     3721 tcgaaccttc agggccgaaa aggttccact ttgctacatg gccgagttcg cctgggcctc
     3781 caccgaatcg aggaagatca tcaagggtga ggaacggatc gtcaggttcc ttgacgaaaa
     3841 gggaatggtt cgcgaaacgg ccttcaggca cggcaaggcg gaatgttgcc agcactgcac
     3901 cttcgacggc atctgcgcgg gcctgttcga tatggacgtg cattacaaat cctcggagtt
     3961 gtacccggtt tttcttgatc ccgaaccgat tcgacagaag gtgctgtcgg aaccataaaa
     4021 cttcaggata cggggctg
//
LOCUS       DAOPPA010000299         4034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24158_length_4034_cov_3.333752, whole genome shotgun sequence.
ACCESSION   DAOPPA010000299 DAOPPA010000000
VERSION     DAOPPA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24158_length_4034_cov_3.333752"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PKH54_09460"
     CDS             <1..1099
                     /locus_tag="PKH54_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00163.1"
                     /translation="QEGTLCYSRERSGFEDGLDVWNATGRPCRPMDCDEESGKFCDGF
                     KFCDTLPGKCGGPGVCVNPNFPDHMTGPENGWQFVCGCDGVTYSGWEQRARAATSVRG
                     EGPCCDESKLNFTRDNAPGFSEFRVCMHGKLPSGLSPSIKAVLDAATFGTAGEGAQCP
                     EGDSAWVGKLTKAPDGSIDPDQFMALCRLADIDDQFAVTGHAEGFCESIPCYDKRKCV
                     ELYCLSPCGCCPCQANKLICGYNNETTICDEKAGCWRRGPECTEWAPCTDGEETDFCR
                     IDCETMAWYLNNAATNPTSCFDDDDCVVMPAFCDPEDGPCWVPFGKYPEWYRDERDFA
                     ADWTSNCVDFESCGCGDRPAARCIGGTCRAG"
     gene            1399..2034
                     /locus_tag="PKH54_09465"
     CDS             1399..2034
                     /locus_tag="PKH54_09465"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOD00164.1"
                     /translation="MLSEKTVERLSEYRRHLSDLQARGVTHVFSNLLAAETRVTAAQV
                     RRDMMNLGLSGSTATGYSVSDLLEHLAGLLDHPHSTRVALIGTGNLGRALLAFFRARP
                     GKAVIAMAFDSDPEKSNRVCCGCRVYPMDSLEARIREDEIRTAILCVPEQDANAVAQR
                     LVNAGVTGILNFAPTRLKVGDQVYVDTMDISSVIEKVCYYAVRGTRAPGRK"
     gene            2049..2516
                     /gene="nuoE"
                     /locus_tag="PKH54_09470"
     CDS             2049..2516
                     /gene="nuoE"
                     /locus_tag="PKH54_09470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOD00165.1"
                     /translation="MNDQIEQILGRFADAERDSLIPILQEVQASEGYLSRDAVVRVAK
                     HLNIAPSKVYGVATFYNQFRFQARGKYNIQVCRGTACHVKGSAAVLEALKRNLKIEPG
                     QTTKDGLFSLEIVACIGACGLAPVIAVNGEFHAQVTPDKITRILNTYRRRAEA"
     gene            2513..>4034
                     /locus_tag="PKH54_09475"
     CDS             2513..>4034
                     /locus_tag="PKH54_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOD00166.1"
                     /translation="MSPDVIINGNQGNGNQGKPGPQLKAELLNIISGAVSERHDALRM
                     VRREVVAKPVIFMGTGTCGLGAGAAATLEGIRGYLNEHDIDADIVEVGCIGLCACEPL
                     MDVQLPGRPRLSFKHVTVDRVHPILDAVFQDELPEETIRKDVLLQFNPGDEDQAWDGV
                     VTMKDHPFFGPQTRWVLENCGLIDPSSVTEYVARGGYKALASMIHSVTPGEVCSLVEQ
                     SGLRGRGGGGFKTGTKWKFAHEAQANQKYLICNADEGDPGAFMDRAVIEGDPHRLVEG
                     MAIAAYAIGASKGYVYIRAEYPLAIKRLKEALGVARELGLLGHNILNSGFDFEIKIKE
                     GAGAFVCGEETALINSIEGKRGMPRPRPPYPAVKGLFGKPTVINNVETLANLPILMRN
                     GAEWFSSVGTAGSKGTKVFALSGKVVNTGLVEIPMGTSIGDIVMKIGGGVPGEHQFKA
                     VQIGGPSGGCIPSQHIDTLVDYESLKTVGAMMGSGGLVVMDEKTCMVDLAKFFMDFI"
BASE COUNT          887 a         1194 c         1230 g          723 t
ORIGIN      
        1 ccaggaggga accctttgct actcaaggga aagaagcggc ttcgaggatg ggctcgacgt
       61 ctggaacgcg accggacggc cttgccggcc gatggactgc gacgaggaat ccggaaaatt
      121 ctgtgacgga ttcaaattct gcgacacgct gcccgggaaa tgcggtggac ctggagtctg
      181 cgtgaatccg aactttcccg accacatgac aggccccgaa aacggctggc agttcgtctg
      241 cggatgcgac ggcgtcacct actccggctg ggaacaacgc gcccgggccg ccacgtcggt
      301 caggggcgag ggtccctgct gcgacgaatc gaagctgaac ttcacccggg acaacgcccc
      361 cggcttttcc gaattccgcg tgtgcatgca cggaaagctg ccatccggac tttccccttc
      421 aatcaaggcc gtgctcgacg cggcaacatt cggtacggcc ggtgagggcg cgcagtgccc
      481 cgaaggcgat tcggcctggg ttggcaagtt gaccaaggca cctgacggct caatcgaccc
      541 ggatcagttc atggcacttt gcagacttgc ggacatcgac gatcagttcg cggtcaccgg
      601 acacgcggaa ggcttctgcg aatcgattcc atgctacgac aagcgcaagt gcgtcgaact
      661 ttactgcctg tcgccatgcg gatgctgccc gtgccaggcg aacaagctca tttgcggata
      721 caacaacgaa acaacaatct gcgacgaaaa ggccggctgc tggcgcaggg gacccgaatg
      781 caccgaatgg gcgccgtgca ccgacggcga ggaaaccgat ttctgccgga tcgactgcga
      841 aacgatggcc tggtacctga acaacgcggc gacaaacccg acttcatgct tcgacgacga
      901 cgactgcgtg gtcatgccgg ccttctgcga tcccgaggac ggcccctgct gggtgccgtt
      961 cggaaaatac cccgaatggt atcgtgatga acgggacttc gccgcggact ggacatcaaa
     1021 ctgcgtcgat tttgagtcgt gtggctgcgg cgaccggcct gcggccagat gcattggcgg
     1081 gacatgccgc gccggctgaa atccgcttca gccacgctcc ggaccacctg attccaaccg
     1141 acaacgtaaa gccgaaatcc atgccgatgc tcgcggggcc gcaaacgacc tgattgaacg
     1201 cgattcgcga cagcccaatg tgcaagcctg cgtattattg tccgtttcac atcatttaca
     1261 ttctttacca gaataccctt gcaatttttc cagcttgtga cataattcac aacgtaagct
     1321 gacagaggtc gcaactctgt tagaaaaccg cacaacccgg acaattcgcg tccggattgc
     1381 tgaagcaggg gttacagaat gctttcggag aagacagtcg agcgcctgag cgaatacagg
     1441 cgacaccttt cggatcttca ggcccgcggg gtgacgcacg tcttttcaaa cctgctggcc
     1501 gccgagaccc gggtaacggc cgcgcaggtg cggcgcgata tgatgaacct ggggttgtcc
     1561 ggcagcaccg ccacgggata ctcggtttcc gacctgctgg aacacctggc cggactgctg
     1621 gatcaccctc attcaaccag ggtcgcgctg attggaacgg gaaacctggg acgcgcgctg
     1681 ctggccttct tcagggcaag gccgggaaag gccgtcattg caatggcatt cgacagcgac
     1741 ccggaaaaat cgaacagggt gtgctgcgga tgtcgcgtgt atccgatgga ctcgcttgag
     1801 gcaagaatca gggaagatga aatccggacc gccattctgt gcgtcccgga acaggacgcc
     1861 aacgctgtcg cgcagaggct tgtgaatgcc ggtgtaaccg gaatcctgaa ttttgccccg
     1921 accaggctga aggtcggtga tcaggtctat gtcgacacga tggatatcag cagcgtgatc
     1981 gaaaaggttt gctattacgc cgtcaggggc acaagggccc cgggcagaaa ataacttatg
     2041 gtggcacgat gaatgatcag atcgaacaga tcctcggacg gttcgcggat gctgaaagag
     2101 acagcctgat cccgatcctg caggaagtac aggcaagcga aggctacctg tcacgggacg
     2161 ctgtcgttcg cgtcgcaaag cacctgaaca ttgcaccaag caaggtttac ggggtcgcga
     2221 ccttctataa ccagttcagg tttcaggctc gtggcaagta caacatacag gtctgccgcg
     2281 ggacggcctg ccacgtgaag ggttcggccg ccgtcctgga ggcgctcaag cgcaacctga
     2341 agatcgagcc gggacagacg accaaggatg ggctcttcag cctggaaatc gttgcgtgca
     2401 tcggggcctg cggacttgcc ccggtcatcg cggtcaacgg cgaattccat gcccaggtga
     2461 ccccagacaa gatcaccagg attctgaaca cttaccgcag gagggccgaa gcatgagtcc
     2521 cgacgtcatc attaacggca atcagggcaa cggcaatcag ggcaaacccg gcccgcaact
     2581 gaaggccgaa ttgcttaaca tcatttccgg cgcggtcagc gaacggcacg acgcgcttcg
     2641 gatggtcagg cgtgaagtcg tggcgaagcc cgtgatcttc atgggaaccg gcacatgcgg
     2701 acttggcgcc ggggcggcgg ccacgctcga aggcatccgc ggatacctta acgagcacga
     2761 tatcgacgcg gacatcgtgg aagttggctg tataggcctg tgcgcgtgcg aaccgctcat
     2821 ggatgtccag cttcccggca ggccgcgcct ttcgttcaag cacgtcaccg tcgatcgcgt
     2881 gcatccgatt cttgacgctg tgttccagga tgaactcccc gaagaaacca tccggaagga
     2941 cgtacttttg cagttcaacc cgggcgacga ggatcaggcg tgggacggcg tcgtcacaat
     3001 gaaggatcac ccgttcttcg gtccgcagac ccgctgggtt cttgaaaact gtgggctgat
     3061 cgatccatcc agcgtcaccg aatacgttgc ccgcggcggc tacaaggcac ttgcctcgat
     3121 gatccacagc gtcacccccg gcgaggtatg cagccttgtc gagcagagcg gcctgcgcgg
     3181 acgcgggggc ggcggcttca agacgggcac caagtggaag ttcgcgcatg aggcccaggc
     3241 caaccagaag tacctcatct gcaacgcgga cgagggcgac ccgggcgcct tcatggatcg
     3301 cgccgttatt gaaggcgacc cccaccgcct tgtcgagggc atggcgattg ccgcctacgc
     3361 aatcggcgcc tccaagggat atgtgtatat ccgtgccgaa tacccgctgg caatcaagcg
     3421 cctgaaggaa gccctcggcg tcgccaggga acttggcctt ctgggtcata acatcctcaa
     3481 cagcggtttc gacttcgaaa taaagatcaa ggaaggcgcc ggggctttcg tctgcggcga
     3541 ggaaaccgcc ctgatcaact ccatcgaggg caagcgcgga atgccgaggc ccaggccgcc
     3601 ctacccggcc gtcaagggac tcttcggcaa gccgacggtg atcaacaacg tcgaaaccct
     3661 ggcaaacctg ccaatcctga tgcgcaacgg cgccgaatgg ttcagcagcg tcgggacggc
     3721 cggcagcaag ggaaccaagg tcttcgcgct gtcgggcaag gtcgtgaaca ccgggctggt
     3781 tgaaattccg atgggaactt caatcggtga catcgtcatg aagatcggcg gtggcgtccc
     3841 cggcgaacac cagttcaagg cggtacagat cggcggcccg tccggcggtt gcatcccgag
     3901 ccagcacatc gacacgctgg tcgattacga atcgctcaag acggtcggcg ccatgatggg
     3961 ctcgggcggt ctcgtggtca tggacgaaaa gacctgcatg gtcgacctgg caaagttctt
     4021 catggacttc atcc
//
LOCUS       DAOPPA010000300         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24268_length_4018_cov_3.359324, whole genome shotgun sequence.
ACCESSION   DAOPPA010000300 DAOPPA010000000
VERSION     DAOPPA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24268_length_4018_cov_3.359324"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..672
                     /locus_tag="PKH54_09480"
     CDS             253..672
                     /locus_tag="PKH54_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD00167.1"
                     /translation="MKSTTRYLEFTVPQRRGFINITRTVEQIVAESGIREGLCLVNAM
                     HITASVFINDDESGLHADYDQWLETLAPHEPVSRYRHNLSGEDNGDAHLKRQIMGREV
                     VVAITNGRLDFGPWECIFYGEFDGRRRKKVLVKVIGE"
     gene            complement(712..1125)
                     /locus_tag="PKH54_09485"
     CDS             complement(712..1125)
                     /locus_tag="PKH54_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOD00168.1"
                     /translation="MDERTLCHECGAEMVLDTRPQEISYKGLCTTIMQRGWYCTNCSE
                     VLLNWDEVEGSFEAFARMKAQADNLLLPKEITRIRKKLKLSQRKASAILGGGPHAFQR
                     YESGRDWITRSMANLLRLLDNDPNRLNELLPRSEK"
     gene            complement(1129..1440)
                     /locus_tag="PKH54_09490"
     CDS             complement(1129..1440)
                     /locus_tag="PKH54_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HOD00169.1"
                     /translation="MYNEKRRPHYDLRAFQAAFARRNGRTMTASAWTSAMLMGFSPDG
                     VCAVIESMLPGHFYKSMTANAEHDVWQDVYHVPFAGICLYVKFTKDRVADFRLLSFKE
                     K"
     gene            1682..2230
                     /locus_tag="PKH54_09495"
     CDS             1682..2230
                     /locus_tag="PKH54_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00170.1"
                     /translation="MKHVIQVIAAAAIIVAAGCESGPDCTTVCNDFTQCGPQSTMYDK
                     CIMRCLNGFFEDACLECSDFGSETDGACTELRTCFYNNCFAWDVGIPAIDPAIQAQAC
                     ANPPPDGTPNADMQDCGIWRELISMCKGDDAMAAGGEAECKKSYNSPECTACLDLKDC
                     TDLLACNVLNCRWTIMAGCTFE"
     gene            complement(2235..>4018)
                     /locus_tag="PKH54_09500"
     CDS             complement(2235..>4018)
                     /locus_tag="PKH54_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00171.1"
                     /translation="DPGWNVPKVLLTEKELNRSWSYPFVYQTSGGAWQRSEWPLESLD
                     DGLSDSYKYPFEVTVPRPTPAGAAASLIWSMNLDAESDAFDLAHFGWGFGDGPFSVCF
                     CDPGDPVELFGLQIDGNVWVGDGNLSPLVKFSRNGRMSYDRPELLAPIDGETDEFVGM
                     NRVYGWVADESRMTFLTNHSGYRLDLVTITPPKVARDAAADIEGARLGDVTTAEPLSM
                     QPVALADGTIMMDAGARYDELDKHSLDSLSALYMAEPPKGQGGAVWTKAMTAQPQVVF
                     EAPRQFFQVASRPGTVFGLMREANTGTDLLVESSDGGSNWTNRRQLAGYVFKAVQVGD
                     GLAFVTHQPVQGADGGRPTEVGIWYLADMTVAEPQPVELAPRIPVPANLYSTIKSCLN
                     ILPMKDGFMVISSLDDAWRSGELDIRRFDASGNLVDSNVTGVRQEMELDVSTAVLAGS
                     EQSPVLVMLRIDAKGPGIQYTAHYSSDLFRDEAQAILPSYWEPPMVPVRLDDGRVAFM
                     GTMNAGADLERAAWTVSADGVSWSEPALIRPEGGYDQEIWGATATPDGRVVVIVGDNE
                     LMRAGGNGIKQTMNGVALRLAPPSISD"
BASE COUNT          882 a         1244 c         1115 g          777 t
ORIGIN      
        1 aaaaccaagg ccaggaaggt cgggaaccag ctttgcggga attccggcaa agtggcggca
       61 gggtagttga aatcttaagg aaaatcaaat aattacttca cgcattgaaa agttggtcgc
      121 atcgattcat cccaatgtgc tcaataatcg gatttggggc tgatacaatc cgcctggcga
      181 caggggccgt cgaaaccggg tcagatcacc ggagcgggaa tccgggacgg cttgcaacaa
      241 ggaagtcaaa ccatgaaaag caccacaagg tatcttgaat tcacggttcc ccagcgcagg
      301 ggtttcatca acatcacgcg caccgtcgaa cagatcgtgg ccgaaagcgg catccgtgaa
      361 ggactgtgcc tggtcaacgc catgcacatc accgcaagcg tcttcatcaa cgacgacgaa
      421 tccgggctgc acgcggacta cgaccagtgg cttgaaacgc tggccccgca cgagccggtt
      481 tccaggtacc gccacaactt aagcggcgaa gacaacggcg acgcccacct taagcgccag
      541 atcatggggc gcgaggtcgt cgtcgccatc acgaacggca ggctcgactt cggtccctgg
      601 gaatgcatct tctatggcga attcgacggc cgccgcagga aaaaggtcct ggtcaaggtc
      661 atcggggagt agaagccgcg gggagtaagc tccgggggga gtaaggccgg gtcacttttc
      721 cgatcgcgga agcagttcat tcaagcgatt ggggtcgttg tccagcaacc gcaacagatt
      781 tgccatcgaa cgggttatcc agtcccggcc gctttcgtac cgctggaagg cgtgcgggcc
      841 cccgcccagg atcgcactgg ccttgcgctg cgacagcttc agcttcttgc ggatgcgggt
      901 gatttccttt ggcagaagaa gattgtcggc ctgggccttc atccttgcaa aggcttcgaa
      961 actgccctcc acttcatccc agttcaaaag gacttcgctg cagttggtgc aataccatcc
     1021 acgctgcatt atcgtggtgc aaagtccctt gtaggaaatc tcctggggac gggtgtccag
     1081 aaccatctcg gccccgcatt cgtgacagag cgttctttca tccatgatct atttctcctt
     1141 gaacgacagc aacctgaagt cagccaccct gtctttcgtg aatttcacgt acagacaaat
     1201 tccggcaaag ggaacatggt aaacatcctg ccagacatca tgttcggcgt ttgccgtcat
     1261 cgatttgtag aagtgacctg gcaacatcga ctcaatcacg gcacagactc catcaggcga
     1321 aaatcccatc agcatggcac tggtccaggc cgaagcggtc attgtgcgcc cgttccgcct
     1381 tgcaaatgcc gcctggaaag ctcgcaggtc ataatgcggc cgccgctttt cgttgtacat
     1441 tgtggcacct tcaaggttgc agatcaagac tcttcatggt gacgcctgga cacacgggga
     1501 catctttcga cagggatctc aatctgttgc atgttcaggt gaatcgcggc agaaggtggc
     1561 tggttcttgt gcccggaccg gagcccgtca ctgaattgac accggcaagg tttgtaaaga
     1621 tacttcgccc ataactcatg acgatcgccg ccattgcggc gacaaaacca ggggacattt
     1681 catgaaacac gtcatccagg tcatcgccgc ggccgccatc atcgtggcgg caggctgcga
     1741 aagcggcccg gactgcacca cagtctgcaa cgacttcacc cagtgcggcc cacagtcaac
     1801 gatgtacgac aagtgcatca tgcggtgcct taacggattc ttcgaggacg cgtgccttga
     1861 atgctcggac ttcggttccg aaaccgacgg cgcctgcacc gagcttcgga catgctttta
     1921 caacaactgt ttcgcatggg acgtcggcat ccccgccatc gacccggcca ttcaggccca
     1981 ggcctgcgca aatcccccgc ccgacggcac gccgaacgcc gacatgcagg actgcggaat
     2041 ctggcgcgag ctgatttcga tgtgcaaggg cgacgacgcc atggcggcgg gcggcgaggc
     2101 cgagtgcaag aaatcgtaca actcgccgga atgcacagcc tgcctggatc tgaaggactg
     2161 caccgacctt ctggcctgca acgtccttaa ctgccgctgg accatcatgg ccggctgcac
     2221 gtttgaataa gggttcaatc agaaattgaa ggcggtgcca ggcgcagggc gaccccgttc
     2281 atggtctgct tgataccgtt gccgccggcg cgcatcaact cattgtcacc gacgattacg
     2341 acgacacggc cgtccggggt cgccgtcgct ccccagattt cctggtcata tccgccttcc
     2401 ggacggatca gggctggctc cgaccacgaa acgccatccg ctgaaacggt ccatgcagcc
     2461 cgttccagat ccgcccccgc gttcatcgtt cccatgaacg caaccctgcc atcgtccagc
     2521 cgcacgggaa ccattggcgg ctcccagtat gaaggcagga tcgcctgcgc ttcgtcgcgg
     2581 aacaaatccg atgaatagtg cgccgtgtat tgaatgccgg gccccttggc gtcgatccgc
     2641 agcattacaa ggacgggcga ttgctccgaa ccggccagaa ccgcggtcga aacgtccagt
     2701 tccatttcct ggcgaacccc cgtcacattt gaatcgacca ggtttccgga agcgtcgaag
     2761 cgacggatgt ccagttcccc ggaacgccag gcatcatcaa ggctggagat aaccatgaaa
     2821 ccatccttca tcggcaggat gttcagacag gacttaatcg tggaatacag gttcgccgga
     2881 accggaatcc tcggggccag ttcaacgggc tgcggctcgg caaccgtcat gtccgccaga
     2941 taccagatgc caacctctgt tggacggccg ccatcggcgc cctgaaccgg ctgatgcgtc
     3001 acgaacgcca gcccatcacc gacctggaca gccttaaaaa cgtacccggc aagctgcctg
     3061 cggttcgtcc agttgcttcc gccgtcggat gattccacca gcagatcggt gcctgtattg
     3121 gcttcgcgca tcaatccaaa aacggtgcct ggcctggaag caacctggaa aaactggcgc
     3181 ggcgcctcaa agacgacctg gggctgggcc gtcatcgctt tcgtccatac ggcgcccccc
     3241 tgccccttcg gtggttcggc catgtaaagc gcgctcaagc tgtccagcga atgtttatcc
     3301 agttcgtcgt acctggcgcc ggcatccatc atgatcgtgc cgtcagccag cgcgaccggc
     3361 tgcatcgaca ggggctcggc cgtcgtgaca tcccccagcc gggcgccttc aatgtctgcg
     3421 gcagcatccc gcgcgacctt cggtggcgta atcgtaacca gatccagccg atagcctgaa
     3481 tgattcgtca ggaaggtcat ccgcgattcg tcggccaccc atccatagac gcggttcatt
     3541 ccgacgaact cgtcggtttc gccgtcgatt ggcgccagca attccggcct gtcgtatgac
     3601 atgcggccat ttctgctgaa cttgacaagc ggcgacagat ttccgtcgcc gacccagacg
     3661 ttgccatcaa tctgaagccc gaacaactcg accgggtcgc cggggtcaca gaaacagacc
     3721 gaaaacggcc cgtcaccgaa gccccacccg aaatgagcca gatcgaaagc gtcggattcg
     3781 gcatcaagat tcatcgacca gatcaggctg gcggccgctc ccgccggtgt cgggcgcgga
     3841 accgtgacct caaacggata tttgtacgaa tcggacagac cgtcatccag gctttccaag
     3901 ggccattcgg aacgctgcca ggccccgcct gacgtctgat aaacgaatgg atacgaccag
     3961 gaccggttca gttccttttc ggtcagcagg accttcggaa cgttccagcc cggatccg
//
LOCUS       DAOPPA010000301         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24433_length_3997_cov_3.430492, whole genome shotgun sequence.
ACCESSION   DAOPPA010000301 DAOPPA010000000
VERSION     DAOPPA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24433_length_3997_cov_3.430492"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="PKH54_09505"
     CDS             <1..2324
                     /locus_tag="PKH54_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOD00172.1"
                     /translation="LDGLAPADFAVTTPRAAEVTTAFQIMAFRQYASTAIAALRRVDP
                     DHLVLGCRFAAYPGDALIEASAEYFDVISMAGYHSNWVDELDAIYPRVDKPFLIEEFS
                     FKATDSGLMNILFYAIVVETQKDRALAFHDYVEELVRRPYTVAYHWYKWMDNPPLETN
                     VLAGDNFGLLNFSDEPYTDFVEYARRVNYLAENWHAEGDGRFNPDRIEFPYCEVDEDA
                     IDAIFDSMTVRQRIGQHIITQITRSGSAVDQDSLQKMEAGLMGGVFVSQITGVATGDP
                     VKTARFVRHAQRTSMELTGVPMFVASDQEGGVYTSVNAMTGGTDSIGPTAVGSTRDDT
                     VSFRQFDMMGREVAALGINMNFGPLLDTHYVIDNGNLNTRTFGPDTDLNARLGLAAVY
                     GFQQNLVLPTAKHFPGDGMAAGNTHHEFVINDASLQELEAALLKPFRTAFDRGCDGVM
                     IMPAQFSAIDDQRAAITSAPVMRDFLRGEIGFDGLIVTDDLNMYGARLGLAEGQEPGY
                     QALKNGADMLLYVSSDPAMLSDLIDRIEAGLMSGPEDPEHIDPEAFEESTKRILRHKQ
                     KYCLFSRDYEDSTAAGAVAGRIGLPADHEMSLAHARLAVAIESDDGVLPLSGKRVLCI
                     GPDAILPDPASGWSWLLGGTFCQAMTALDPNVLAMNYFVDGGEESAGDWVIENTELAD
                     VVVVATFQGWFSQAQQALLDRVVSGSGLPVVHVAQGVPFDLVQTAATASARVALMGSL
                     PIMFQAGAEILFGLSEGGGLIPWNLDIPFRKRK"
     gene            2371..>3997
                     /locus_tag="PKH54_09510"
     CDS             2371..>3997
                     /locus_tag="PKH54_09510"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOD00173.1"
                     /translation="MDTVESRGYVVLKFGGTSVSSLARWQTIAEQVRQRLQEGLRPLV
                     VCSAFATVSNSLEELLAAAASGRHEPILQGIVRLHTEMAETLGLDATALLQADLDEIG
                     RLAFGASLTQEVSPRLKARVMAFGELISTRLGAAWLNGQGIETAWLDARDYLRAESCP
                     DTNPYRHYLNATCGFERSPGLIAMLDSVPAPVLITQGFIARDSAGDTVLLGRGGSDTS
                     ASYLAALLGAKRCEIWTDVPGIYSANPRQVPTARLLKHLDYDEAQEITSTGAKVLHPR
                     CIPPVRAHGIELHIRCSQMPDAVGTVITSQTEGQGAQVKAISQKSQVTLISMETLGMW
                     QQVGFLADVFAVFKRHGFSIDLVSTSEMNVTVTLDPASNSLNPDLVNDLLRDLGQYCR
                     ARQISDCAVVSLVGRHIRAIMHRLGPALELFQEQKIYLVSQAASDLNLTFVVDREQAD
                     RLVGQLHELLFMGRVRDSLLGPSWQEMFERPAEGESEEVPWFNGRRQELLDIAAGGPA
                     YVYDQGTLMADARALKGLKSADRILYAVKANGNA"
BASE COUNT          739 a         1164 c         1300 g          794 t
ORIGIN      
        1 aactggacgg gctggcgcct gcggatttcg ccgtcacgac gccccgggcc gccgaggtga
       61 cgacggcgtt ccagatcatg gctttcaggc agtatgcttc gacggcgatc gcggcacttc
      121 gcagggtcga tccggaccac ctggtgcttg ggtgcaggtt cgccgcgtat ccgggcgacg
      181 cgttgatcga agcttccgcc gagtatttcg acgtcatatc catggccggc taccattcga
      241 actgggtgga cgaactggac gccatttatc cccgcgtcga taaaccgttc ctgatcgagg
      301 agttctcgtt caaggctacc gattccggcc tgatgaacat cctgttctat gccatcgtgg
      361 tcgagacgca gaaggacagg gcgctggcct ttcatgacta cgtcgaggaa ctggtccgcc
      421 ggccgtacac cgttgcgtac cactggtaca agtggatgga caatccgccg ctggagacga
      481 acgtccttgc gggggacaac ttcggtctgc tgaacttttc cgacgagccg tacaccgatt
      541 tcgtcgaata cgcccgcagg gtgaattacc tggcggaaaa ctggcacgcg gaaggcgacg
      601 gccgtttcaa tccggacagg atcgaatttc cgtattgcga agtcgatgag gacgcgattg
      661 acgccatttt cgattcaatg acggttcgcc agcgaatcgg gcagcacatt atcacgcaga
      721 taaccaggtc gggcagcgcg gtcgaccagg acagcctgca gaagatggaa gccgggctta
      781 tgggtggtgt ttttgtcagc cagattaccg gcgtcgcgac cggagatccg gtcaagacgg
      841 ctcgcttcgt caggcatgcc cagcgtacct ccatggaatt gaccggcgtc ccgatgttcg
      901 tggccagcga tcaggagggc ggtgtttaca cgtcggtcaa cgccatgacc gggggcaccg
      961 acagcatcgg gccgaccgcg gtcggctcga cgcgcgacga cacagtgtcc ttccggcagt
     1021 tcgacatgat gggacgcgag gtcgcggcgc ttggcatcaa catgaatttc ggtccgttgc
     1081 tggatacgca ttacgtgatc gacaacggga acctgaacac caggacgttc ggcccggaca
     1141 ccgacttgaa cgcccgtctg gggcttgccg ccgtctacgg gttccagcaa aacctggtgc
     1201 tcccgaccgc caagcacttt cccggcgacg ggatggccgc agggaatacc caccatgagt
     1261 tcgtcatcaa cgatgcgtcg ttgcaggaac tggaggcggc gttgcttaag cctttcagga
     1321 ctgctttcga ccgtggctgt gacggcgtga tgataatgcc ggcgcagttt tcggccatcg
     1381 acgaccagcg ggcggccata acttcggcgc cggtgatgcg tgatttcctg cgcggcgaaa
     1441 tcggtttcga cggcctgatc gtgacggacg atcttaacat gtacggcgct cggctgggtc
     1501 tggccgaagg acaggagccc ggataccagg ccctgaagaa cggcgcggac atgctgcttt
     1561 atgtttcatc cgacccggcg atgctttcgg atcttatcga caggatcgag gccggcctta
     1621 tgtccgggcc cgaggatccg gagcatatcg acccggaggc cttcgaggaa agcacgaaga
     1681 ggattcttcg ccataagcag aagtattgtc tgttttcccg tgactatgaa gattcgaccg
     1741 cggcgggcgc ggtggcaggc aggatcgggc tgccggctga tcacgaaatg tccctggccc
     1801 acgcccgcct ggccgttgct atcgagtccg atgacggggt gcttccgttg tccgggaagc
     1861 gggtcctgtg catcggcccg gacgcgatcc tgccggatcc ggcatccggg tggtcctggc
     1921 tgcttggcgg cacattctgc caggccatga cggccctcga tcccaatgtc ctggccatga
     1981 actattttgt tgacggtggc gaggaatctg ccggtgactg ggtcatcgaa aacacggaac
     2041 tggccgatgt ggtggtcgtg gccacattcc agggctggtt cagtcaggcc cagcaggccc
     2101 ttctggaccg agtcgtttcc ggttccgggc ttccggtggt gcacgtggcc cagggcgttc
     2161 cgttcgatct tgtccagact gccgcgacgg catcggcccg cgtggcactg atgggcagtc
     2221 ttccgatcat gttccaggcc ggcgccgaga tcctcttcgg tctttcggaa gggggcggcc
     2281 tgattccctg gaacctggac atcccctttc gaaagcgcaa gtgaccgcat aactttttcg
     2341 taaacaaaac caacaatttg agaggttcaa atggataccg tggaatcccg tggatacgtc
     2401 gtgctgaaat ttggcggcac cagcgtttcg tcgcttgcgc ggtggcagac catcgccgag
     2461 caggttcggc agaggctgca ggaaggcttg cgtccgctgg tggtgtgctc ggccttcgcg
     2521 actgtgtcga actcgctcga ggaactcctg gccgccgcgg catccgggcg tcatgagccg
     2581 attctgcagg gaattgtccg gcttcacacg gaaatggccg agaccctggg acttgacgct
     2641 acggcgcttc tgcaggccga tcttgacgag atcggtcgcc tggcgttcgg ggccagcctt
     2701 acgcaggagg tttcgccacg cctgaaggcc cgcgtgatgg cgtttggcga actgatatcg
     2761 accaggctgg gggcggcctg gcttaatgga cagggcatcg agaccgcatg gctggacgcg
     2821 cgcgactacc tgcgggccga atcctgcccg gacacgaacc cttacaggca ttacctgaac
     2881 gccacctgcg gatttgagcg aagtcccgga ctgatcgcga tgctggattc ggttccggcg
     2941 cccgtcctga taacccaggg ttttatcgca agggattccg ccggtgacac ggtgctcctg
     3001 ggcaggggcg gatcggacac cagcgcgtcc tacctggcgg cgctgcttgg cgcgaagcgc
     3061 tgcgagatat ggacagacgt cccgggcatc tactcggcga accccaggca ggtcccgact
     3121 gcaaggctgc tgaagcacct ggattacgac gaggcacagg agattacttc caccggggcg
     3181 aaggtgctgc acccccgctg cataccgccg gtgcgggcgc acgggatcga actgcatatc
     3241 aggtgctcgc agatgcccga tgccgtcggc acggtgatta cctcacagac cgaagggcag
     3301 ggcgcccagg tcaaggcgat cagccagaag agccaggtga cgctgatttc gatggaaacg
     3361 ctggggatgt ggcagcaggt cggcttcctg gcggacgtgt tcgcggtgtt caagcggcac
     3421 ggattttcaa tcgacctggt atcgacttcc gagatgaacg tgaccgttac gctggatccg
     3481 gcgtccaatt cgctgaatcc ggatctggtg aacgacctgc ttcgcgacct tggccagtac
     3541 tgccgggccc gccagatttc cgactgcgcc gtcgtcagcc tggtcgggcg tcacatcagg
     3601 gctatcatgc atcgcctggg accggcgctg gagcttttcc aggagcagaa gatctatctg
     3661 gtttcccagg ctgccagcga tctgaacctt acgttcgtgg tcgatcgcga gcaggcggat
     3721 cggctggtcg gccagcttca cgaactgctg tttatgggac gtgtgcgcga cagcctgctt
     3781 ggaccgtcct ggcaggagat gttcgagcgg cccgcggaag gtgaatccga ggaagttccc
     3841 tggttcaatg gcaggcggca ggaattgctg gacatcgcgg ccggcggtcc ggcgtacgtc
     3901 tatgatcagg gcacgctgat ggccgacgcg cgagcgctga agggactgaa atcggcagat
     3961 cggatcctgt acgcggtcaa ggccaacgga aatgcgg
//
LOCUS       DAOPPA010000302         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24848_length_3946_cov_3.766127, whole genome shotgun sequence.
ACCESSION   DAOPPA010000302 DAOPPA010000000
VERSION     DAOPPA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24848_length_3946_cov_3.766127"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..431
                     /locus_tag="PKH54_09515"
     CDS             207..431
                     /locus_tag="PKH54_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00174.1"
                     /translation="MRKAFLLVLGLMTMLFAACDTGLPRFTPAEGSYTEGGGTNRYSF
                     VGGGTGNYTAPDGGFVIDAGGDTGGEEEEE"
     gene            474..1109
                     /locus_tag="PKH54_09520"
     CDS             474..1109
                     /locus_tag="PKH54_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00175.1"
                     /translation="MNRFSSSSPIFALVLVAMLIPACGCGQKTVDLTSPEAAYESFRA
                     HLLRGDWEGVFPLMIPEVKDNITRTWQMNRKTASLIESSIPAALKQNYLSEIGSAEIR
                     NAASPQQYFGALMRSAFPVSLVPGDSRHSVETSISRISETRKGSRNYVVTTIAGRQIN
                     LVLNQDNTYGIEPPDDQARLFRMNYKAASELYINVKAVVDSLDSETGKAPR"
     gene            1106..1933
                     /locus_tag="PKH54_09525"
     CDS             1106..1933
                     /locus_tag="PKH54_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOD00176.1"
                     /translation="MIRLRYVAIISMLLSCAAAGSQVKEDDSPTPGENDQGVEESPPA
                     EETVETATDESGQAPAWATIQFVTPRGEPMTLRFPVRSSSIGSPSSGSLVNGKDIGRS
                     GPGFIQTGTAPFGTDETVMLVMFAAGELLRDYPNSVPALVGSLSDADGGPIPPHKSHQ
                     SGRDVDIAFFARENVPLRTFGDLDPAQIDFEKSLALLVNLVSTGRVSFIFINYALQKF
                     FFQAARDMGYSDAQLDYLFQYPKGESAKSGIIRHAKGHFRHAHVRFACPKGSQDCRE"
     gene            complement(1934..3694)
                     /locus_tag="PKH54_09530"
     CDS             complement(1934..3694)
                     /locus_tag="PKH54_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOD00177.1"
                     /translation="MRKSTRILLLAFVMLLAGFGATYLSRRLDQRDTLLIPAIADKSS
                     VRLIELSSGSRLVKLSRLPDGEWSFFPPAGARADRLAVNGLLDAFSSDLVSVIPVDTE
                     AGAAEYGLDPDTAIRVAFDIGSGSSQDIEIGSVQDNPDAGPDTFVRMSGADQVFRLTG
                     HDLRRPFPRGLDSMRSSRLFSFGVGDVKTVTFSRSDGDSKWEAVTVTYEGDRKSPLAR
                     MGWRIVSPGGWEPGGIEEVVGQASLVLVDSWAESLPEGVFISSPVAKVTFGLVDGRTV
                     EVVLSAESAGFVWVATSEVAGFARLRSADAGLLAKSLFDLADLSLVKSSPEEIESVLI
                     EGEGRRLAIRREGAGIVSDGMPAPLDRSEVETWLRSISEMDASGVISGKAAASAFQSD
                     LRLKVTRTDRSVISVSFGPRERDGRRHAMVDGLPGIAVVTAAELAPLDITVDRLMARS
                     VFPRDSSTLRKIRVWSPVFGTAVIRPPEKGEKNFSVQFGNSPAISSQAVTSIATAAIS
                     MRAHSFVDDVRPSRIRADHLSVTFTAEGGDIVLKVSPAERGGGFVAATTGNRFMDSRA
                     FVIPASMIDAVSAVFAIREL"
     gene            complement(3718..>3946)
                     /locus_tag="PKH54_09535"
     CDS             complement(3718..>3946)
                     /locus_tag="PKH54_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00178.1"
                     /translation="PVVAQNMGFVYNCLDWGVMNEALVDIRSKTDDRRPIDQVTPVAA
                     RIIELAFIIGLPMLFAGIGVVRHRMRRNRPW"
BASE COUNT          853 a         1202 c         1136 g          755 t
ORIGIN      
        1 gcgacggggg gtcgaagccg tggcaacaaa ttccagggtt cttttcgagg atgccattga
       61 cgatacggcc aagggactga agaagatggc cgccagaacc gggattggcg aatcgccgcg
      121 acgatgacgt cttttcaagg tgtcgcgaat ttgtaacgcg cgcttgtccg gcgatagaat
      181 cctgtgtgat gaatggaggg tacgccatga gaaaggcgtt tctgcttgtg ctcggcctga
      241 tgacgatgct tttcgcggcc tgcgataccg ggcttccccg cttcacgccc gcagaaggtt
      301 cctacaccga gggcggtggg acgaacaggt attccttcgt cggcggcgga accggaaact
      361 acacggcgcc tgacggtgga ttcgtgatcg acgccggcgg cgacaccggt ggggaagaag
      421 aagaggagtg atttccgctt cctcctttcc gttcccgttg cagcgaggcc ggtgtgaacc
      481 gtttttcatc gtcatccccg attttcgccc tcgtgcttgt cgcgatgctg attccggcat
      541 gcggatgcgg ccagaagacc gttgacctga cctctcccga ggccgcatac gaatcgtttc
      601 gggcgcacct gttgcggggt gactgggaag gtgtctttcc cctgatgatc cccgaggtca
      661 aggacaacat cacaagaacc tggcagatga accgcaagac tgccagcctg attgagtcca
      721 gcattccggc ggcgctgaag cagaactacc tttccgagat cggatctgcc gaaatcagga
      781 atgcggcgtc gccgcaacag tacttcggtg cgttgatgcg atccgctttc ccggtgtcgc
      841 tggttccggg tgactcaagg cactcggttg aaacgtccat ttccaggatt tcggaaacca
      901 ggaagggaag tcgcaattac gtcgtcacca cgattgcggg acgccagatc aaccttgtcc
      961 tgaatcagga caacacttac ggaatcgagc ctcccgacga ccaggcccgt cttttccgaa
     1021 tgaactacaa ggcggccagc gagctttaca tcaacgtcaa ggccgtcgtc gattcgcttg
     1081 attcagaaac cgggaaagcc ccccgatgat aaggcttcga tacgtcgcca tcatttcgat
     1141 gctcctctca tgcgcggccg cgggaagcca ggtgaaggaa gatgattccc cgacgcccgg
     1201 cgaaaacgac cagggagttg aggaatcgcc gccagcggaa gagaccgtcg agaccgcgac
     1261 agatgaatcc ggacaggcgc cggcctgggc gacgatccag ttcgtcaccc ccaggggcga
     1321 gccgatgacg ctcagatttc ccgtgcgttc aagttcgatt ggcagccctt cgtcgggaag
     1381 cctggtcaat ggaaaggata tcggaagaag tggccccggt ttcatccaga ccgggacggc
     1441 ccctttcggg acggatgaga ccgtcatgct tgtgatgttc gcggccggcg agcttctgcg
     1501 ggactacccg aactcggtgc cggcccttgt cggatcgctg tcggacgctg acggaggccc
     1561 gattccgcca cacaagtcac accaatccgg ccgcgatgtc gatatcgcat tttttgcccg
     1621 tgaaaacgtg cccctgcgaa cattcggcga tcttgatccg gcacaaatcg acttcgaaaa
     1681 gtcgcttgcg cttctggtga acctggtttc gacgggacgc gtcagcttca tcttcatcaa
     1741 ttacgcgctg cagaagttct ttttccaggc cgcgcgcgac atgggctaca gcgacgctca
     1801 gttggattac ctgtttcagt acccgaaggg ggaaagcgcg aaaagcggca tcatcaggca
     1861 tgcaaagggc cacttcaggc acgcgcatgt aaggtttgcc tgcccgaaag gctcccagga
     1921 ctgtcgggaa tgatcacagt tccctgatgg cgaataccgc ggaaaccgca tcaatcatcg
     1981 aagccggaat cacgaaggcc cggctgtcca tgaaacgatt gccggtcgtg gcggcaacaa
     2041 atccaccgcc tctttcggcc ggggaaacct tcaggacgat atcgccgcct tcggccgtga
     2101 aggtgaccga aaggtgatcc gccctgatcc gggacggccg cacatcatcg acaaatgaat
     2161 gggcgcgcat cgaaatcgcc gcggtcgcaa tcgatgtcac ggcctgggag gatatggccg
     2221 ggctgtttcc gaactggacg ctgaagttct tttcgccctt ttcgggcggc cttatcactg
     2281 cggttccgaa caccggcgac cagacgcgga tcttcctgag agtcgatgaa tcgcggggaa
     2341 agacggatct tgccatcagc cggtcaacgg tgatgtccag cggcgcgagt tcggctgcgg
     2401 tcacaaccgc gatgcctggc aagccatcaa ccatggcgtg ccttcgtcca tcccgttccc
     2461 tcgggccgaa cgaaacggaa atgacacttc tatcggttct ggtcaccttc aatcgaagat
     2521 cggactggaa ggcggaagcc gccgcctttc cggaaatcac gcctgaagca tccatctcgc
     2581 ttatcgaacg cagccaggtc tcgacctcgc tgcgatccag gggcgccggc atgccgtcgg
     2641 agacaatccc ggcaccttcc cttcgaatcg ccaggcgacg gccctcgcct tcgatcagga
     2701 cggactcgat ttcttccggc gatgacttca caaggctcag atccgccaga tcgaaaagcg
     2761 attttgcaag aagcccggcg tcggcggaac gaaggcgcgc gaacccggca acttcgctgg
     2821 tggcaaccca gacaaatccg gcgctctcag ccgaaaggac aacttccacc gtgcgtccat
     2881 cgacaagccc gaaggtcacc ttcgcgaccg gggaagagat aaaaacccct tcgggaagcg
     2941 attcggccca cgaatcgaca agcacaaggc ttgcctggcc gacgacctct tcaattccac
     3001 caggctccca cccgccaggc gacacgattc gccagcccat cctggcaagg ggactttttc
     3061 tgtcaccttc gtatgtcacg gtgacagcct cccatttcga atcgccatcc gatctggaaa
     3121 acgtgactgt ttttacgtcg ccgacaccga aggaaaacaa ccttgacgac cgcatcgaat
     3181 ccaggcccct gggaaagggt ctgcgaagat cgtgcccggt cagcctgaag acctgatctg
     3241 cgcccgacat gcgcacgaaa gtgtccgggc ctgcatcggg attatcctgc acggaaccga
     3301 tttcaatgtc ctgtgaagac ccgctgccga tgtcgaaggc aacccggatc gccgtgtcgg
     3361 ggtcaagccc gtattcggcc gccccggcct cggtgtcaac cggaatcact gaaaccaggt
     3421 cgctggaaaa ggcatccagc agaccgttca ccgcaagccg gtcggcacgc gcgccagccg
     3481 gcgggaagaa tgaccattca ccatcgggca gccttgaaag cttcaccagg cgggatcctg
     3541 aggacagttc gataagacgg actgacgact tgtcggcaat cgccggaata agcaaggtat
     3601 ccctttgatc cagcctcctg gacagatacg ttgcgccaaa ccctgcaagc agcatcacaa
     3661 aggccagcag gagtatcctg gtgctcttcc tcaagtttcc cccggacggc ttcgaattca
     3721 ccagggcctg ttgcggcgca tcctgtgtcg gacgaccccg atccctgcaa acagcatggg
     3781 aaggccaatt atgaaggcaa gctcgattat ccgcgccgcc accggcgtaa cctggtcaat
     3841 cgggcgcctg tcgtcggtct tggatctgat atcgacaagc gcctcgttca tcacgcccca
     3901 gtcgaggcag ttatacacaa accccatgtt ctgggcgacc accggc
//
LOCUS       DAOPPA010000303         3936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_24936_length_3936_cov_2.686421, whole genome shotgun sequence.
ACCESSION   DAOPPA010000303 DAOPPA010000000
VERSION     DAOPPA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24936_length_3936_cov_2.686421"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PKH54_09540"
     CDS             <1..1525
                     /locus_tag="PKH54_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00179.1"
                     /translation="DEYALIRCLHAPNDLATILEILLASNLGGLLDQIPPISVPIPNI
                     VNFSILGVGLQGDVNIEVQLRDISFGEPYVQILTREGGISTNVTMQPVTVGMDLKFTI
                     KARAVAFGNTYDLLDPSTSSGSSMEIGTFGLGLSIDINKTPGQDVTIEGKDFELTIQD
                     IQLDPIEHLEIDLGTIGPLGIDLGVVDLTRIVGSIDDLLMNWVLEPILNFLTPLLTNL
                     LEPLVTELMGTLLTTLFDQLVLNQTVELPELAAGSGTTPMDLSLAPSTIVFTPDGGTI
                     GMELGFLTAREVEHEIPGVIGSLTESAGDAFAFDRDPGVQAAIDIQTINTLLFMIWQS
                     GMISGQIDLSSLVEGVGMGVGNLFVTPDLYLPPIINDSAVGEDGMMSLEIGDAYIKLQ
                     VDLLGNPQFIDLWLQMAIQVQIVMKGNEVGIRFGDVTFFQTEFGDLGDLEGLVGMFLP
                     MIPDLIKGIEGQEFVFPIPEIDLSSIIPGLGGSAVIQLGNGLSTVRDGMVVFGADLI"
     gene            complement(1546..2220)
                     /locus_tag="PKH54_09545"
     CDS             complement(1546..2220)
                     /locus_tag="PKH54_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD00180.1"
                     /translation="MGEVNYAEKEVTTVSQLIPLVQKLACRFRRDSWSAADRDDIRQE
                     GFVGLIEAARRFERSRGCSLRTFGGRRAAGAMMDYVRTLCRKSRERSTEDLPSEECRL
                     RRAQPDYETPGSMESGVMLLRFRQFLAGEDGGLGLLPDDEREVIRLRFFEGLSCRESA
                     EAMKVSPATICRLERRALDKLRQGFVSSCGVSESGCDAYDADEAGEEDAAPSSGFSGI
                     IRMLTI"
     gene            2561..>3936
                     /locus_tag="PKH54_09550"
     CDS             2561..>3936
                     /locus_tag="PKH54_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOD00181.1"
                     /translation="MLNRKFLAAMMLFAWVGCGSDVVENTDEGIQGDSNVEDVLEPID
                     ANLPDGAICSPGTGRCSGNNWLVCNDEGTNYDVIPCDGACDNETGCLGDTTCTPLQTR
                     CNQDGKMQVCLPDGSEWSEATDCPDEGQTCIAGACISSSCMPGATQCQGNTLLICNDE
                     SGTWLSEVCGPKAICFKGDCVECVTEDQCARGSKCFDGHCQPVPLSILTADLPEGQIN
                     IAYTASMEAMGGQPDYEWSVTSGAMPAGITLGRDGAIAGTPTAAGTFTVTIAVTDSVD
                     AVVQKPYEIVIHEEGLSIVSKSPLPDAEEGTDYSFQFKALGGVPPYGWMLTSGALPKG
                     LSITSDGMLSGIPEEHGDFTFTMRAIDVGEPLSYDTREFKLHVAIAPLVIYGDQEINL
                     ILAKAIILPLITTGLGLPYNQQLQAKGGVKPYTWAESSMSSLLQMLIPKSGIPEGLTL
                     SVSGVLSGR"
BASE COUNT          801 a         1238 c         1125 g          772 t
ORIGIN      
        1 ggacgaatac gccctgatcc gctgtttgca cgccccgaac gacctggcca cgatcctgga
       61 aatccttctt gcaagcaatc ttggcggcct gctcgaccag atcccgccga tatcggttcc
      121 gatccccaac atcgtcaatt tcagcatcct tggcgtcggg ctgcagggcg acgtgaacat
      181 cgaggtgcag ttgagagaca tcagcttcgg cgaaccttac gtccagatcc ttacgcgcga
      241 aggcggaatt tccacaaacg ttacgatgca gccggtgacg gtcggcatgg acttgaagtt
      301 caccatcaag gccagggccg tggctttcgg caacacgtac gatctgctgg atccaagcac
      361 ctcgtccggt tccagcatgg aaatcggcac gttcgggctg ggactttcga tcgacatcaa
      421 caagaccccg ggacaggacg tcacgattga aggcaaggat ttcgagctga caatccagga
      481 catccagctt gacccgatcg aacaccttga aatcgacctg ggcaccatcg gcccgctcgg
      541 gatcgacctt ggcgtggtcg atctgacacg catcgtcggc agcatcgacg accttctgat
      601 gaactgggta ctcgagccga ttctgaactt ccttacgccg ttgctgacga accttctgga
      661 accgctggtc accgaactga tgggcaccct cctgacgacc ctgttcgacc agcttgtcct
      721 taaccagacc gtcgaactgc cggaactcgc ggccggcagc ggcaccacgc cgatggatct
      781 gtccctggcg ccttcgacaa tcgtcttcac gcccgatggc ggaacgatcg gcatggaact
      841 tggattcctg acggcgcggg aagtcgagca cgagattccc ggggtcatcg gcagcctgac
      901 cgagtcggcc ggcgacgcgt tcgccttcga ccgcgacccg ggggtgcagg cggcaatcga
      961 tatccagacg atcaacacac tcctgttcat gatctggcag tccggcatga tcagcggaca
     1021 gatcgatctt tcatcgctgg tcgagggtgt cggcatgggc gtcggcaacc tgttcgttac
     1081 gccggatctg tatcttccac cgatcatcaa tgactctgcc gtcggcgaag acgggatgat
     1141 gagcctggaa atcggcgacg catacatcaa gctccaggtc gatcttctgg gcaatccgca
     1201 gttcatcgac ctgtggctgc agatggccat ccaggttcag attgtcatga agggtaacga
     1261 agtcggtatc aggttcgggg acgtcacctt cttccagacc gaattcggcg accttggcga
     1321 ccttgaaggg ctggtcggca tgttcctgcc gatgattccg gatcttatca aggggattga
     1381 aggccaggaa ttcgttttcc cgattccgga gatcgacctg tcgtcaatca ttcccggact
     1441 tggcggttcg gcggtcatcc agcttggcaa cggactctcg accgtccgcg acggcatggt
     1501 ggttttcggt gccgacctga tctagcttca gggcccggcc ttcagtcaaa tcgtcagcat
     1561 cctgattatt ccggaaaacc ccgaactggg ggcggcatct tcttcaccgg cttcatcggc
     1621 gtcataggca tcgcatccgc tttcagatac cccgcatgac gaaacgaagc cctggcgcag
     1681 tttgtccagg gccctgcgct caagcctgca tatcgtcgcc ggcgaaacct tcatcgcctc
     1741 ggccgattca cggcaggaca ggccttcgaa aaagcgcagc ctgattactt ccctttcgtc
     1801 gtccggcagc aggcccaggc cgccatcctc tcccgccagg aactgccgga accgcaggag
     1861 catgacaccg gattccatgg agcccggagt ctcatagtcc ggctgtgcgc gccgaagccg
     1921 gcattcctcg gacggaagat cctcggtcga gcgttcacgg gacttcctgc aaagggtcct
     1981 gacgtaatcc atcatcgcgc cggccgcccg gcgtccaccg aacgtgcgca ggctgcaccc
     2041 tctggatctc tcgaaacgac gggccgcctc gataaggccg acaaaacctt cctggcgaat
     2101 gtcgtcgcga tccgcggcgc tccaggaatc gcgcctgaac ctgcatgcca gtttctgcac
     2161 cagcgggata agctgagata cggtggtgac ttccttttcc gcatagttga cttcgcccat
     2221 gatttcctcc gtgggaagcg tgtgtcgatg accgggtatt tcaagcttcg tgccagaacg
     2281 gaggcctgcg gaaggccaac tgcgaatcac cgataacaaa ctgtaattta tggataaatc
     2341 cggacgatcg ctttcatctg cacggatcgg ccccaggttc ggccgcccgg ccaacggggt
     2401 gtccgtgccc caccggcggt gcattcagcc cccaccaccg gaacggcccg gaattacgat
     2461 tcgtttacac cggcaaagct ggaaattgaa tacctcaaag cctataatcg cggcactccg
     2521 tttggagact ttccgaaacc ccattttcag agggcctgca atgctcaaca gaaagttcct
     2581 tgccgcaatg atgttgttcg cctgggtcgg ctgcggttcc gacgtcgtcg aaaacaccga
     2641 cgaaggcatc cagggcgatt cgaacgtaga agacgtcctt gaaccaatcg acgccaacct
     2701 tcccgacggg gccatctgct cacccgggac cggtcgctgc tccggcaaca actggctcgt
     2761 ctgcaatgac gaaggcacga attacgacgt cattccctgc gacggcgcct gcgacaacga
     2821 gacgggctgc cttggcgaca ccacctgcac gccgcttcag accagatgca accaggatgg
     2881 caaaatgcag gtctgcctgc ccgacggatc cgaatggtcc gaggccaccg actgcccgga
     2941 cgagggtcag acctgcatcg ccggcgcctg catttccagc agttgcatgc ccggcgcgac
     3001 acagtgccag ggcaacaccc tgcttatctg caacgatgaa tcgggaacat ggctgtccga
     3061 ggtctgcgga ccgaaggcca tctgtttcaa gggcgactgc gttgaatgcg tcaccgaaga
     3121 ccagtgcgcg cgcggcagca agtgctttga cggccactgc cagccggtgc cgctttcgat
     3181 tctgacggcc gacctgccgg aaggccagat caacatcgcc tacacggcct cgatggaggc
     3241 gatgggcggc cagccggact acgaatggag cgtgacatcc ggcgccatgc cggccggaat
     3301 cacccttggc agggacggcg cgattgccgg aacgccgacg gcggccggca ccttcacggt
     3361 aaccattgcg gtcaccgact cggtcgatgc cgtcgtccag aagccttatg aaatcgtgat
     3421 tcacgaagaa ggcttgagca tcgtcagcaa gtcgccgctt ccggacgccg aggaaggaac
     3481 cgattattcc ttccagttca aggcacttgg cggcgtgccg ccctacggct ggatgctgac
     3541 cagcggcgcc ctgccgaaag gtctttcgat tacctccgac ggcatgctgt ccggcatccc
     3601 cgaggagcat ggcgacttca cgttcaccat gagggcgatt gacgttggcg agccgctgtc
     3661 ctacgacacg cgcgaattca agctgcacgt cgcgattgcg ccgctggtga tctatgggga
     3721 tcaggaaatc aacctgatcc ttgcaaaggc catcatcctg cccctgatca cgacgggcct
     3781 gggccttccc tacaatcagc agttgcaggc aaagggtggc gtgaagccct atacgtgggc
     3841 ggaatcatcg atgtcctcgc tcctgcagat gctgattcca aagtccggca ttcccgaagg
     3901 cctcacgctc agcgtctcgg gcgtgctttc gggccg
//
LOCUS       DAOPPA010000304         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25012_length_3927_cov_3.869835, whole genome shotgun sequence.
ACCESSION   DAOPPA010000304 DAOPPA010000000
VERSION     DAOPPA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25012_length_3927_cov_3.869835"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="ffh"
                     /locus_tag="PKH54_09555"
     CDS             complement(<1..1499)
                     /gene="ffh"
                     /locus_tag="PKH54_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOD00182.1"
                     /translation="MFDVLTKGFRGARAKLQGRAIIDEAAISDILKDIRVSLLEADVA
                     LPVVNAFLDRVREKALGEVVQVKFKDKAGAKQVSPADHFVKLCNDELIALMGEGQRDL
                     VWAKDGRPTMVMMVGLHGTGKTTTSAKLAKLLKDQKKKPMLVAADIYRPAAIEQLKIL
                     GQKIGVPVFSVPGMSPPDLCEASIKHAAGQNCNVIIFDTAGRLAIDEPLMDELGQIKA
                     RTRPTNIFLVVDAMIGQDSVRMATEFDRRLDITGVVMTKMDGDARGGAALSVKGVTGK
                     PIVFAGMGEAMDKLEPFRPEGIATRILGFGDVVGLVQDFEKVVDEQKAEEDAMKILKG
                     EFTLYQFMDQIKAIQKMGSLKGTLEKLPFFHEMVPEGATVDEKSLSRIEAMIHSMTPD
                     ERNKPAIILAGGKNDSRAKRIARGSGRKPEEVKDLVKRFETMREIMKQVGKNPSLLGR
                     LPGFSQAMELARERGENVDDFMGGGKMPKGYGNPYGAGPRKTSQADKDKR"
     gene            1796..1945
                     /locus_tag="PKH54_09560"
     CDS             1796..1945
                     /locus_tag="PKH54_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00183.1"
                     /translation="MNAILLAASRAGTTEAKVVVVVRRVVVVAIGRAEVVVVVVEATA
                     ASDPV"
     gene            complement(1995..3014)
                     /locus_tag="PKH54_09565"
     CDS             complement(1995..3014)
                     /locus_tag="PKH54_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOD00184.1"
                     /translation="MNHEPQYQNVCSFSALHAAFRKARKAKRGKNGEPAFYLELESNL
                     LDLSDQLRTHAFVPEPYRYFSLHNKKDRVVSVASFRDRVVHHALVAELEPLYEQLFIP
                     TSFACRKGLGMHRALDLAHELSRRNGYFLKLDIRKYFDHISHPLLLGILANDVHDERI
                     LWLCDVLLANAGVPGVEPTERRGVPIGNLTSQFWANVYLNRLDQAFARQFTGLPYLRY
                     MDDILIFGFDKDELWSAHGFINSYVDLVLALELKASATVVAPVTEGIPWLGFRVYPGT
                     VRLDHGSRTRFLRKVRGNFLAAANAMDDEMAAVVASAQSIFGHIGHCNSLMFRRSVLA
                     RHGRD"
     gene            complement(3011..3379)
                     /locus_tag="PKH54_09570"
     CDS             complement(3011..3379)
                     /locus_tag="PKH54_09570"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOD00185.1"
                     /translation="MQTTRDFLLLSAAFPKRFRSSLTARMENLFLEIAELLTTASYTR
                     NRSMLFDRIDDTLNRLRVQIRLSHEVGILSDAQYQGLAKALTETGRMVGGWKKADSNK
                     ISGYQDVQTNDQPSVFPTES"
     gene            complement(3285..>3927)
                     /locus_tag="PKH54_09575"
     CDS             complement(3285..>3927)
                     /locus_tag="PKH54_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOD00186.1"
                     /translation="GQELVLGDSMSFSEARILTIQWDPRTGMFDDSVLRNYLINRELV
                     RCVPQFFVHNALPCWSLYIETKVLQGVVSSDLSASSRQAAAAGIAAQINEFDEIQKVR
                     YERILSWRAEAAMREGLKSYMICTNRDAMELARTAPATLAALGRVHGFGEKKLAKYGR
                     EILEILHGTHKTSGNSNNIRKVDADDKGLSAVVGGVSQAVPVIADGKDGEPVP"
BASE COUNT          839 a         1161 c         1049 g          878 t
ORIGIN      
        1 cgcttgtcct tgtcggcctg ggaagtcttg cgcggacctg cgccgtaagg attcccgtac
       61 cccttcggca tcttgccgcc gcccatgaaa tcatcgacgt tctcgccgcg ttcgcgcgca
      121 agttccatcg cctgggaaaa accgggcagg cgaccgagca gggacggatt ctttccgacc
      181 tgcttcatga tctcgcgcat cgtttcaaaa cgcttgacca ggtcttttac ttcctcgggc
      241 ttgcggcccg aaccgcgggc gatacgtttc gcacggctgt cgttcttgcc gccggccagt
      301 attatcgcgg gcttgttgcg ttcatccggc gtcatcgaat gaatcatcgc ttcgatgcgg
      361 gacagcgact tttcgtcaac ggtcgcgcct tcgggaacca tttcgtggaa gaacggcagc
      421 ttttcaaggg tgcccttcag ggatcccatc ttctggatgg ccttgatctg atccatgaac
      481 tgatacaggg tgaactcacc cttcagtatc ttcatggcat cttcttcggc cttctgctcg
      541 tcaacgacct tctcgaagtc ctggaccagg ccgaccacgt cgccgaatcc aaggatgcgg
      601 gtcgcgatgc cttccgggcg gaagggttcc agcttgtcca tcgcctcgcc cataccggcg
      661 aaaacgatcg gcttgcccgt gacgcccttg accgacagcg cggcgccacc gcgcgcgtcg
      721 ccgtccatct tggtcatcac gacgccggtt atgtccagcc ggcggtcgaa ctcggtggcc
      781 atccggaccg agtcctggcc gatcatcgcg tcgacgacca ggaagatgtt ggtcgggcgg
      841 gtgcgggcct ttatctggcc aagttcgtcc atcaggggtt cgtcaatcgc caggcggccg
      901 gcggtgtcga agatgatgac gttgcagttc tggccggcgg catgcttgat cgacgcttca
      961 caaaggtcgg gcggcgacat cccgggaact gaaaagaccg ggacgccaat cttctggccc
     1021 aggatcttca actgctcgat tgcggcgggg cgatagatgt cggccgcgac cagcatcggc
     1081 ttctttttct gatccttcag cagcttggcc agcttggccg aagtggtcgt ctttccggta
     1141 ccgtgcagac cgaccatcat caccatggtc gggcggccgt cctttgccca taccagatcg
     1201 cgctggccct cgcccatcag ggcaatcagt tcgtcgttgc acagcttgac gaagtggtcg
     1261 gcagggctga cctgcttggc gcctgccttg tccttgaact tgacctggac gacctcgcca
     1321 agtgcctttt cgcggacgcg atcaaggaac gcgttgacga ccggcagcgc cacgtcggct
     1381 tcaagcaggg acaccctgat atccttcagg atgtcggaaa tcgccgcctc gtcgatgatg
     1441 gcccggccct gcagttttgc ccgggcgccc cggaatcctt tcgtcagaac gtcgaacata
     1501 tcgcaaaatc ccctttcgga tgacagtgtt tcgcagcgct tcaagccgga aaaacaaagg
     1561 ttttccggcc catcgcttat agttgaaaaa gccccgtttc gtcaagggaa ggggcggaat
     1621 cgatccaaga tccatcgcta cttggacgga cggatttcat ccgccctgcg caacctgcga
     1681 tttgccgtcc cggggtgccg gattcggcac cccgggacgg ctgggatctt cgtccggctg
     1741 cgatgccggc gctggtttta cggccatggc cgcaacatgc actgggatcg aatccgtgaa
     1801 cgcgatcctt ctggcggcgt cgcgggctgg cacaacggaa gccaaggttg tcgtcgtcgt
     1861 ccgacgggtt gtcgtcgttg cgattggacg cgcggaggtt gttgtcgtcg ttgtcgaagc
     1921 taccgccgcg tctgacccgg tttgagccgc agggccgcaa aaccaccgat attgaaacac
     1981 agaacgcaaa agaatcaatc tctgccgtgc cgggcaagca cgctgcgcct gaacataaga
     2041 ctgttgcaat gtccgatgtg accaaaaatg ctttgcgcgg aggcaacgac ggctgccatt
     2101 tcatcgtcca tcgcattggc ggccgccaga aaatttccgc gcaccttgcg aagaaaccgg
     2161 gttctggaac cgtgatccag ccgaacggtt cctggataaa cacgaaatcc aagccagggg
     2221 atcccttccg ttaccggcgc caccacagtt gccgaggcct tcaattccaa tgcgagcacc
     2281 aggtcaacat acgaatttat gaaaccatgg gccgaccaga gttcgtcctt atcgaatccg
     2341 aaaattagaa tgtcatccat gtaacgcaaa tacggcaatc cggtaaactg tcgggcgaaa
     2401 gcctgatcca gtcgattcag atagacattg gcccagaact ggctggtcag gttgccaatt
     2461 ggcactccgc gcctttccgt cggttcaact ccgggaacac cggcatttgc cagcaacacg
     2521 tcgcaaagcc acaggatcct ctcgtcgtga acgtcattcg ccaaaattcc cagcaataac
     2581 gggtgactta tatgatcgaa gtacttgcga atatccagtt tcaagaaata gccattgcga
     2641 cgggacaact cgtgcgccag atccagggca cggtgcatcc ccaacccttt ccgacatgcg
     2701 aatgacgtcg gaatgaacag ttgttcgtat aaaggttcaa gttcggcaac cagtgcgtgg
     2761 tgaacaacac ggtcacggaa agacgcgacc gacaccacac gatccttctt gttatgaagg
     2821 ctgaaatacc gataaggttc aggtacaaag gcatgcgtcc tcaactggtc ggacaaatca
     2881 agcaggttgg attccaattc cagataaaag gcaggttcgc cgttttttcc ccgcttggcc
     2941 tttctggcct ttctgaacgc ggcatgaagg gccgaaaacg aacacacgtt ctgatattga
     3001 ggttcgtgat tcatgattca gtcggaaaaa ctgaaggttg atcatttgtc tggacatcct
     3061 ggtaaccaga gattttgttt gaatcagctt tcttccatcc gccaaccatg cgccctgttt
     3121 cggtcaaagc ctttgcaagg ccttgatatt gcgcatctga caggataccg acttcatgac
     3181 tcaaccgaat ctgaacccgt aatcggttta acgtgtcatc gatccgatca aacagcatgc
     3241 tccgatttct tgtatatgac gccgttgtaa gcaattcggc gatctcaagg aacaggttct
     3301 ccatccttgc cgtcagcgat gaccggaacc gcttgggaaa cgccgccgac aacagcagaa
     3361 agtcccttgt cgtctgcatc cacttttcga atattattag agtttccaga tgttttgtgc
     3421 gtcccatgca gtatctccag aatttcgcgg ccgtatttcg ccagcttttt ttcgccgaac
     3481 ccgtgaaccc gccccagggc cgcaagcgtc gccggcgccg tccgcgcaag ttccatggca
     3541 tcgcgattcg tgcaaatcat gtaagacttc agaccctcac gcatggcggc ctccgctcgc
     3601 catgacagga tccgctcgta cctgactttc tgaatttcat caaactcgtt gatctgcgcg
     3661 gctattccgg cggccgcagc ctgtcgggac gaggccgaca aatcggatga gacgacaccc
     3721 tgaagtacct ttgtttcgat atacaacgac cagcagggaa gcgcgttgtg tacaaaaaac
     3781 tgcggaacgc aacgaaccag ttcacggttg atcaggtagt ttcgcagaac cgaatcatcg
     3841 aacatcccgg ttcggggatc ccattggatt gtcaaaattc tggcttcact gaacgacatc
     3901 gaatcaccca ataccaattc ctggcca
//
LOCUS       DAOPPA010000305         3925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25028_length_3925_cov_4.191731, whole genome shotgun sequence.
ACCESSION   DAOPPA010000305 DAOPPA010000000
VERSION     DAOPPA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25028_length_3925_cov_4.191731"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKH54_09580"
     CDS             complement(<1..682)
                     /locus_tag="PKH54_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00187.1"
                     /translation="MKRTIIASILVFMFNVPALAQEQDKTTAEPVATVESAEAPAPAN
                     TAEGDAPSVEPAAGPAGAIEPEPLEGDPIDQADAESQDEASGTEVEPPTPDELLPPEQ
                     KGFGIGNKYARFMGRLQTIFLWRSDSDFDQTPPLYNANGQDVGVVGTFLAPMLVVTPV
                     KELKMVFELEMGLNIWSTHDPDNYSTSLPDWFRVGFRQAYVEGNFEKQKIGFRVGYVF
                     RFPPLPLQP"
     gene            complement(697..3609)
                     /locus_tag="PKH54_09585"
     CDS             complement(697..3609)
                     /locus_tag="PKH54_09585"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00188.1"
                     /translation="MKRTDLLRGSLATLAVTVALAFLSGCSGEKPDLNDGLKPIVTDL
                     TWFKDTEGRYLNIKGINLGGNIKVPVVRSTRNDETPQTYYGHVGDQIQAMKDGAELPF
                     SYVGRPFSLAKAEEYFKQMKDLGFNSVRLLWLWETVYPEKKFKPDREYLEYFEKIVEL
                     AAKYDIYVMINLHENLWSRTFYSLYSNWPVCQQCCNYNADNFAIDPDGKVTDIVCTDD
                     RKAECCPQGDPMNMLWSVFPSKTRNELGIQASDTPEVKAAKYRAGFNDRVSGDGAPLW
                     ASQVCLPEKNFESPYWGVSKLLGAAVNQTGDILYMDLFTIIKMIAATLANLEEPMITH
                     EIEEQITAQLDRMEPYLPPTAFGSQDTYDGLPFKMWGINNGVSLQTNICFAAFYSGDE
                     VFPNRRTVEYGYSDRLDEGLEIETFYTPEEADARAAELRSAGYTYVKVNNLRESLQEG
                     FRGAWEEIARIGKKYPNVIGYDILNEPAAVYVLMSIVLAYLELGSPDLVASLLDGVVL
                     DDEGLPMKLPGGQTIGEVAQQVIEANLAILPKDNTDETRKMLGLYGADLIKVLGMNMA
                     VDKNCLEPLYEYVGEGILKVYSEDEKTPPANRLTFWLEPAHGIDMLLGGGAGGVGGQY
                     QQYATTPDIPGAPEFEKKFGEIKFVWSPHWYPDIYPMIGLNMPERTFAMDEYDFRDYS
                     SVIKEKADWAKFAYNNIPFVMGEFGTYWNFRYLENDEACDEELKECRLAKDKFLDPAC
                     EPAKFNCPIGYIQSRQFDYEISTQILDNYYENYEQMMHGNMVWVYTPDADPKLGDYWD
                     HEDFSLVEFIQKSREPERYENAFKAIPERYLVKVEQPEGVVAPRGHRAFVRPYAHLIS
                     GKPVSTHFYSDLHYFDPEKGVPNAEREFQIVFKSKETHAPTVIFVPELQYPDGFYVWL
                     SDGYALWDREDQWLYYFPDRDEPGIEHTVTIRPPLQGQNSSDWDYFVNGDFVVAAD"
BASE COUNT          736 a         1122 c         1146 g          921 t
ORIGIN      
        1 aaggttgaag cggtagtggc ggaaatcgaa agacatagcc gacgcggaaa ccgatcttct
       61 gcttttcgaa gttaccttcc acatacgcct gccggaagcc gacgcggaac cagtcgggca
      121 ggctggtgct gtagttgtcc ggatcgtgcg tggaccagat gttaaggccc atttccagtt
      181 cgaacaccat cttcagttcc ttgaccgggg taaccacaag catcggggca aggaatgttc
      241 cgacgacgcc cacgtcctgg ccgttcgcgt tgtaaagagg cggggtctgg tcgaagtccg
      301 agtcgctgcg ccagaggaat atggtttgca ggcgtcccat gaacctggcg tatttgtttc
      361 cgatgccgaa acctttctgt tcggggggca gcagttcgtc cggggttggt ggctcgactt
      421 ccgtcccgga agcctcgtcc tgggattccg cgtccgcctg atcgatgggg tcaccctcaa
      481 gcggctccgg ttcaatcgcg cccgccggcc ctgccgcggg ctcgaccgaa ggagcgtcgc
      541 cttccgcggt gttggccggc gccggtgctt cggcagactc gaccgtcgcg accggttccg
      601 ccgtggtttt gtcctgttcc tgtgcaaggg ccgggacgtt gaacatgaaa accaggattg
      661 atgcaattat tgtgcgtttc atattccatc cccccttcag tccgcggcaa cgacgaagtc
      721 gccgttgacg aagtaatccc agtcgcttga gttctgcccc tgcagcggag gcctgatggt
      781 caccgtgtgc tcaatgcccg gttcatccct gtcggggaag tagtagagcc actggtcttc
      841 acgatcccac agtgcgtatc cgtcggaaag ccacacatag aagccgtccg gatactgaag
      901 ttcgggaacg aaaatcaccg ttggagcatg ggtttccttg ctcttgaaaa cgatctggaa
      961 ctcgcgttcc gcgttcggca cgcccttctc cgggtcgaag tagtgcagat ccgaatagaa
     1021 atgggtgctt accggcttgc ccgagatcag gtgcgcgtac ggcctgacga aggccctgtg
     1081 tcccctgggc gcgacaacgc cttccggctg ttcaaccttg accaggtaac gttccggtat
     1141 tgccttgaag gcattctcgt acctttccgg ttcgcgggat ttctgtatga attcgacaag
     1201 cgagaagtcc tcgtgatccc agtaatcgcc gagtttcgga tccgcatccg gcgtatatac
     1261 ccagaccatg ttcccgtgca tcatctgttc gtagttctcg tagtagttgt ccagaatctg
     1321 cgtgctgatt tcgtaatcga actgacgtga ctggatgtaa ccgattgggc agttgaactt
     1381 cgcaggttcg caggcgggat ccaggaactt gtcctttgcc aggcggcatt ccttcagttc
     1441 ttcgtcgcac gcctcgtcgt tttccaggta acggaagttc cagtacgtcc cgaattcgcc
     1501 catgacgaac gggatattgt tgtatgcgaa tttcgcccag tcggcctttt ccttgatcac
     1561 cgacgagtag tccctgaaat cgtattcgtc catcgcgaat gtgcgttcag gcatgttcag
     1621 gccgatcatc ggatagatgt cgggatacca gtggggtgac cagacgaact tgatttcacc
     1681 gaatttcttc tcgaattccg gggcccccgg gatgtcgggc gtggttgcgt actgctggta
     1741 ctgtccgccg acgccgccgg ccccgccgcc gagcagcatg tcgatgccgt gcgcaggttc
     1801 cagccagaat gtcaggcggt tcgccggggg tgtcttttcg tcttcggaat agaccttcag
     1861 gataccttcg ccgacgtatt cgtaaagcgg ttcaaggcag ttcttgtcaa ccgccatgtt
     1921 cattcccagg acctttatca ggtcggcgcc gtaaagcccc agcatcttgc gggtttcgtc
     1981 ggtgttgtcc ttcggcagga tggccaggtt ggcctcgatg acctgctggg ccacctcgcc
     2041 gatggtctgg ccgccgggca gcttcatcgg gagtccctcg tcgtcgagca cgacgccgtc
     2101 aagcaggctt gccacaagat ccggcgaccc aagttccagg tatgccagga cgattgacat
     2161 caggacatag accgcggcag gttcgttcag gatgtcgtag ccgattacgt tggggtactt
     2221 cttcccgatc ctggcgattt cttcccacgc gccccggaat ccttcctgca gcgattcgcg
     2281 caggttgttg accttcacat aggtgtatcc cgccgaccgc agctcggcgg ccctggcatc
     2341 ggcctcttcc ggggtgtaga aggtttcgat ctcgagtcct tcatcgagcc tgtccgagta
     2401 tccgtattca accgtgcggc ggttcggaaa gacttcatcc ccgctgtaga atgcggcaaa
     2461 acagatgttc gtctgcagcg acacgccgtt gttgattccc cacatcttga agggcagccc
     2521 gtcgtaggtg tcctgcgagc cgaaagcggt cggcgggagg tagggttcca tcctgtcgag
     2581 ctgggcggtg atctgctcct cgatttcgtg ggtgatcatc ggttcttcca ggttggcaag
     2641 ggttgccgcg atcatcttga tgatggtgaa cagatccatg tagaggatgt cgccggtctg
     2701 gttcacggca gccccgagaa gcttgctgac gccccagtaa ggagattcga agttcttttc
     2761 cggaaggcag acctggcttg cccacagcgg tgcgccgtcg ccgcttacgc ggtcgttgaa
     2821 accggcacgg tacttggctg ccttcacctc gggcgtgtcc gacgcctgga tgcccagttc
     2881 gttgcgggtc tttgacggga aaaccgacca cagcatgttc atcggatcgc cctggggaca
     2941 gcattcggcc ttgcggtcat cggtgcagac gatgtcggtc acttttccgt cggggtcgat
     3001 cgcgaagttg tcggcgttgt agttgcagca ctgctggcag accggccagt tgctgtaaag
     3061 gctgtagaag gtcctgctcc acaggttttc atgcaggttg atcatcacgt agatgtcgta
     3121 cttcgcggcc agttcgacaa tcttttcgaa gtattcgagg tattcgcgat cgggcttgaa
     3181 cttcttttcc ggatagaccg tttcccacag ccacagaagc cttaccgagt tgaagccaag
     3241 atccttcatc tgcttgaagt actcttccgc cttggccagg ctgaaaggcc ttccaacgta
     3301 cgaaaacggc agttcggcgc cgtccttcat ggcctggatc tgatcgccga cgtggccgta
     3361 ataagtctgg ggcgtttcgt cgttcctggt cgaacgcacc accggaacct tgatgtttcc
     3421 gcccaggttg atgcctttaa tgttcaggta ccgcccttcg gtatccttga accacgtaag
     3481 gtcggtcacg atcggtttca ggccgtcgtt cagatcgggc ttttcaccgg aacatcccga
     3541 aaggaacgca agggccaccg tcacggcaag cgtggcaagc gaaccccgga gaaggtcggt
     3601 ccgtttcatg tcaatacccc cggaatcaag tcgttgaaaa tcgtccgcgc cgggacgaaa
     3661 agtcgctgca aggataacct gtgtaaagga aaattgccca ttcaaaattc ggccctcccg
     3721 ccggcgtccc gccttgacaa ttgtccagtc gtcgcctaga gtttccccgc gattggcggg
     3781 gcggtaccgc cggcctggcc ggatcagaga aaccgttgga aatccgacgt gttgcgagtc
     3841 ttatcacact agttcaggag aaacgggatg tccagacgag agatggtgac gattgacggc
     3901 aatgaagcaa cggcatccgt ggcac
//
LOCUS       DAOPPA010000306         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25046_length_3924_cov_3.718015, whole genome shotgun sequence.
ACCESSION   DAOPPA010000306 DAOPPA010000000
VERSION     DAOPPA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_3924_cov_3.718015"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKH54_09590"
     CDS             <1..855
                     /locus_tag="PKH54_09590"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOD00189.1"
                     /translation="SPSTFWGVMTTSTRPVIASHSNSASVFRHARNIDDAQLWAIAES
                     GGLVGLNLFSRFISDTEPVASMADLLRHANHVKAVVGASHLAIGSDFDGARYFVAGLE
                     DSSRMGNLAAALLEAGWSKADVQAVLGGNFLRVLDATASGEPSPSVTHMPAQVISVTT
                     DHQGGPVWAVADSDTTTAWIPEGPGDHSVTISAEGPGVDRVSVCGASPGIAAVGYELS
                     VQALCEGQPVGQSGMIAVGDAVRPFRLDLPELKDCRSPSVRLGLPIPKPDRVPARLSE
                     VVLERRVR"
     gene            941..1930
                     /locus_tag="PKH54_09595"
     CDS             941..1930
                     /locus_tag="PKH54_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD00190.1"
                     /translation="MACFVVASAIPVPADAARVRCNQKTLPKLMKNEGFSAPPNFKAV
                     VLKVKFNNARNKVVSWTAYDYNGTSDDRTWNAASSIKFMAAIGALQRTRSLGFAPSAE
                     VTFRGKKKVVTTVRKLVEDAIIDSNNIAYNRLVQLAGFDRLHSKVLVSRYGIADTSLM
                     KAYQQGDWTAAGESIQLREAPEIQLRQGKKKRTIPASIGKLKRNCHSSVCTDLRDIAE
                     AVMTLMIQEQVPAGRSFGLHRTDLDLIRKAMMEDRSRGNEFVDNLRRGLDLKQARFFS
                     KPGYSENWYSSVSYIYIKGRPEAWVVAMAGKPDRDALNSAARIVGKLIRNRRL"
     gene            complement(1953..2768)
                     /locus_tag="PKH54_09600"
     CDS             complement(1953..2768)
                     /locus_tag="PKH54_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD00191.1"
                     /translation="MNKSIRISILLVAAAVMLAFASSAVAGDMSNAGIEAARHGYDAQ
                     ADTRSRPEAPSDGRQDEETTDKADNDCICEYRLYNWNSARGRTEAAEPVRKPCTEVTP
                     DERDPLEPRCTVCRKDQVRLDPATLGMPGVKPFMVCRVFERRVRDALARIAKSGSFEV
                     TEIVGYRPGRTRGPIRDGFRTVLSNHSFGTAIDINPAHNGLYKNCTRTDFKKTGHGKC
                     RLSIGGKWDPSANPRRTITGDSVVVKAFSDFWKWGGDIPGGTRDLMHFSISGF"
     gene            complement(2819..>3924)
                     /locus_tag="PKH54_09605"
     CDS             complement(2819..>3924)
                     /locus_tag="PKH54_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00192.1"
                     /translation="MSLLRPAIMLALFIASSATAMASDVNSDSPAGAADAMAADSDSP
                     ASAADAQAGRAPVLHHLTPEQHDIVWGIKGDPEPEVRNAYRKENEGQHFTTCDELNLH
                     LFEPAIRGIGGAYAGVGTDQAYIFMGWARSEIGWMLDYDPLVIRIHGLYRVFFMEAGN
                     FERFLHLWSAEGAAEAREVIKRRVSFEPAAESALELYAKFRAHIIRRLMQSISLMRKA
                     GVAFYLNDAATYDHVRNLVMTGRAVAVQANLLADGGIKNIGEASRILGVPVRALYLSN
                     VEDYWAYGSQYMNNLQSLPYDDTSMILRTLAAKKSNGDYRYVQQKATNLLKWLESGKV
                     RRTRDMFRRGAIRKSDEVQLVTIETDPDKKSVR"
BASE COUNT          753 a         1125 c         1174 g          872 t
ORIGIN      
        1 tcgccatcga ctttctgggg cgtcatgacc acaagcacgc gtcccgtcat tgccagtcat
       61 tccaactcgg catccgtttt caggcatgcc aggaacattg atgacgccca gctctgggca
      121 atcgcggagt caggcggact ggtcgggctg aacctttttt cgcgattcat ttcggataca
      181 gagcccgttg catcgatggc ggatcttctg cggcatgcaa accatgtcaa ggcggttgtc
      241 ggcgcgtccc atcttgcgat tggaagcgat ttcgacgggg ccaggtattt cgttgccggc
      301 ctggaagatt cttcacggat ggggaatctt gccgccgcac ttctggaagc cggctggtcg
      361 aaggcggatg tccaggccgt ccttggcggg aattttcttc gggtgctgga tgcgacggcc
      421 tcgggtgagc cgtcgccttc ggtcacgcac atgcctgccc aggtcatttc tgtcacgacg
      481 gatcatcagg gcggccccgt gtgggcggtt gcggattccg acacgacaac ggcctggatt
      541 cccgaaggcc cgggggatca ctcggtcacg atcagcgccg aaggtccggg ggttgatcgc
      601 gtttccgtgt gcggtgccag ccccggaatc gcggccgtcg gttatgagct ttccgtgcag
      661 gcgttgtgcg aaggccagcc tgtcgggcaa tctggtatga tcgcggtcgg tgacgccgtg
      721 cggcctttca ggcttgatct tccggaactg aaggactgca ggtcgccatc ggttcgcctg
      781 ggtcttccga ttccgaagcc cgaccgggtt cctgccaggc tgtccgaggt cgtgctggag
      841 cggcgggttc ggtgatgtat tgcggcttgc agatggtgaa gtcttgatcc tgtcgtcgcc
      901 acggttcctg aaaggcgctt gtgtgtcaac ccttgtcgcc ttggcctgct ttgtcgtcgc
      961 ttcggccatc ccggttccgg ccgatgccgc ccgagtcagg tgcaaccaga agacgctccc
     1021 gaagctgatg aagaacgagg gcttttccgc accgcccaac ttcaaggccg ttgtgctgaa
     1081 ggtcaagttc aacaacgcca ggaacaaggt cgtttcctgg accgcatacg attacaacgg
     1141 gacgtcggat gaccgcacct ggaacgcggc gtcttcaatc aagttcatgg ctgccatcgg
     1201 cgccctgcaa aggacaagat cccttggttt cgcaccctcg gcggaagtca cattccgggg
     1261 caaaaagaag gttgtcacga cggtcaggaa gctggtcgag gacgcgatca tcgacagcaa
     1321 caacatcgcc tacaacaggc ttgtccagct tgccggcttt gaccgcctgc actcaaaggt
     1381 gcttgtttcc cgttacggaa ttgcggacac gtcacttatg aaggcttacc agcagggtga
     1441 ctggacggcc gccggcgaaa gcatccagct tcgcgaggcc cctgaaattc agcttcggca
     1501 gggaaagaag aagcggacta ttcccgcgtc catcggcaag ctgaaacgaa actgccattc
     1561 ttcggtctgc accgatctgc gcgacatcgc cgaagccgtc atgacgctga tgatccagga
     1621 gcaggttccg gccggccggt cgttcggcct gcacagaacg gatcttgatt tgattcggaa
     1681 ggcgatgatg gaggatcggt cccgcggaaa cgagtttgtt gacaacctgc gcaggggcct
     1741 ggatctgaag caggccaggt ttttcagcaa gcccgggtat tcggaaaact ggtacagcag
     1801 cgtttcatac atttacatca agggtcgtcc cgaggcgtgg gttgtcgcca tggccgggaa
     1861 acctgatcgc gatgccttga attcggccgc caggattgtg ggaaagttga tacgaaacag
     1921 gcggctgtaa ttcgtcaatg gatacggtgt cttcagaacc cggaaatcga gaagtgcatc
     1981 agatcccttg tgccgccagg gatatcgcca ccccatttcc agaagtccga gaaggccttg
     2041 acgacgactg aatcgcccgt gatcgtcctt cgcggatttg ccgacggatc ccacttcccg
     2101 ccgatcgaga gtcggcattt gccgtgtccc gtcttcttga aatccgttcg cgtacagttc
     2161 ttgtaaaggc cgttgtgggc tggattgata tcgatggcgg ttccgaatga gtggttgctc
     2221 aggacagtcc tgaagccgtc cctgatcggt ccgcgggttc gtcccggacg gtacccgacg
     2281 atctccgtga cttcaaatga gccggatttg gcgattctgg caagggcgtc gcgaaccctt
     2341 cgttcgaaca cgcgacatac catgaacggc ttgacccccg gcattccaag ggtggccggg
     2401 tcgagccgaa cctgatcttt ccggcacacg gtgcatcgcg gttcaagcgg gtcgcgttcg
     2461 tccggcgtga cttcggtaca tggcttgcga acaggctcgg cggcttcggt ccgaccgcgc
     2521 gcggaattcc agttgtaaag ccggtattcg cagatgcagt cgttatcggc tttgtccgtc
     2581 gtttcttcat cctgacggcc atccgaaggc gcttccgggc gactgcgggt atctgcctgg
     2641 gcgtcgtatc cgtgacgtgc ggcttcaatc ccggcgttcg acatgtcgcc cgcgacggcc
     2701 gacgatgcaa aggcaagcat gaccgcggct gccacaagca ggattgatat cctgatggat
     2761 ttgttcaatc gacaaactcc gcttccaccc gacggtcggc acggatccct gaaccggact
     2821 atcttacaga ctttttgtcg ggatcggtct cgatggtcac aagctgcact tcatcggact
     2881 tgcggattgc accgcgtcgg aacatgtccc tggttcggcg aacttttccc gattcgagcc
     2941 atttcagaag gtttgttgct ttctgctgga cataacggta atcgccgttg gacttcttcg
     3001 ccgcaagtgt acgaagtatc atcgaggtgt cgtcgtacgg caggctttga aggttattca
     3061 tgtactggga tccatatgcc cagtaatctt caacgttcga aaggtaaaga gccctgacgg
     3121 gaaccccgag tatcctggaa gcctctccaa tatttttgat tccaccgtct gcaagaagat
     3181 ttgcctgaac cgcaacggca cgtcccgtca tcacaaggtt tctgacgtgg tcgtatgtcg
     3241 ccgcgtcgtt caggtagaag gctaccccgg cctttctcat caggcttatc gattgcatca
     3301 gccgcctgat gatgtgggcc ctgaattttg catacagttc aagggccgac tcggcggccg
     3361 gctcgaacga cacgcgtcgt tttatgactt cccttgcttc ggccgcacct tccgccgacc
     3421 acaggtgaag gaagcgctcg aagttcccgg cttccatgaa aaagacgcgg taaaggccgt
     3481 gaatcctgat gaccagcggg tcgtaatcaa gcatccatcc gatttccgaa cgcgcccatc
     3541 ccatgaatat ataagcctga tcggtgccga ccccggcgta tgcgccgccg attccacgga
     3601 tcgccggttc gaacaggtga aggttcaatt cgtcgcatgt cgtgaagtgc tggccttcat
     3661 tttccttgcg gtacgcgttc cttacttccg gctccgggtc gcccttgata ccccagacga
     3721 tatcgtgctg ttccggggtc aggtggtgga gaacgggcgc ccggccggcc tgggcatcgg
     3781 cagcgctggc aggggagtct gaatcggcag ccatggcatc ggcagcgccg gcaggcgaat
     3841 cggaattcac gtcggacgcc atggccgtgg cggaactggc aatgaacaac gcaagcataa
     3901 tggccggtcg aagcagggac atac
//
LOCUS       DAOPPA010000307         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25080_length_3920_cov_3.946442, whole genome shotgun sequence.
ACCESSION   DAOPPA010000307 DAOPPA010000000
VERSION     DAOPPA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25080_length_3920_cov_3.946442"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3826)
                     /locus_tag="PKH54_09610"
     CDS             complement(<1..3826)
                     /locus_tag="PKH54_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOD00193.1"
                     /translation="MKRPANRHEWTHFPETAGLVASLVLLTAVLPGCDISSFYPDFDI
                     PDITLHDNPFDPPDDWAGNDDGTSAFIIERMEPSSGPASGGTRVHIEGRGFDLGSRVF
                     FGTVEGVETIVESDTSIRTSTPGGSGYSETVDVFIVRPDAKKTVLRPGFMYQADTAVA
                     SISPPSGPVSGGTPVIITGHGFAPDCNVIIDGRHALKVHRLDDSTVLAVTPHGDPGPV
                     EVLVISGGASGRLPGGFSYVSEPRVSGCSPGVISRLGSDGDGNVTFRVEGSGLDAADS
                     ILLSQGSVLGSSVIDGGLEIRAFLGIDAAPGSVDVTVGGPWGVTELPSCLVALDPADL
                     QSGGLKIRSVIPKAADATSGIVGTIHVTGLDVDEPARISLTLGDTPAVVTSIPDATTI
                     RFIIPQGSPGTVDVALQTPSGTAIASKAFTFLAPASISSVNPAECIPGGDTAGSILGS
                     GLAGAVQVGIGPVLAVMTGPPSDDEIRFELPDLEAGVFDVSVLLADGRQIVSKGALTC
                     SARRASILAVSPDRGSLAGGTFAWVTGRGLRPGTRIFIGGAEAEILDGTDPGRIAIRT
                     PPADEAGPAEVFAVTPDGIRERLSYPFNYFDPTSFLGGAWGRPIAGAVNITVLDVYTD
                     KPVPGAFVMLGQDPWTPLKGFTDARGMATLSTRGLTGPVMATASRIDYTTNSLVGTSA
                     ENITFFIEPLIREIPDTGNGGDGETVFPPNPGIITGQVTGIDKEFRAPHSSCAGVPLT
                     DGALCRPCRTDEDCGQGYFCVMSGITALTCSKSCDTSMDCPDLYTCGSTGDEHHFCTP
                     LKGRTEIRCATADSSTTRWAEKYGPGAVPDDRGFFAINSRLGDVAVTCVGGYRNRVDG
                     KFVPTLLGVKRHVQVTSSAISSGNEVRLSIPLDRTLTTSMISAPGSSSGTGNHSATLS
                     LRLGSDGYLNLWHPISIPDGRVFTFTGLPREMGDQLADSEFLVRTEAASPVSNSDPYS
                     TVQTMRFVPENPPVTAVNRLSAESLSLKYRLDAMAACYGNESGIVVDARGNAWEIDNL
                     GGIRALPLVGGGDIRACAFEPDRKVLFAAGGSLVWSFDLVTHEQVPERIGTRLMDLSL
                     IARGDDGTVWVSDGKTIHSKSSDGGWRELTLDVPVELSAMAAGQGGMVAVGKRGMVVR
                     ADGTTATALNPITGKDLIAVAVSPERVFAVGQGGTILHGTDAADLKPIPTGILDDLTT
                     ALVDGGSELVAGGTRGILIRVSDASITTIRQPENQPIVSAALVPVGSADATGYSNILA
                     FDLNAVMTGPFAQ"
BASE COUNT          754 a         1214 c         1229 g          723 t
ORIGIN      
        1 tctgagcgaa aggaccggtc atgacggcgt tcaggtcgaa cgccaggatg ttcgaatatc
       61 cggtggcatc cgcggaaccg accggaacaa gagcagccga tacaatcggc tggttttccg
      121 gctgtctgat tgtcgtgatc gatgcgtccg acacccggat caggattccg cgagttccgc
      181 ccgcgacaag ttcggaaccg ccatcaacaa gggccgttgt caggtcgtcg agaatgccgg
      241 tggggatagg cttcaggtct gccgcatcgg tgccatgcag gattgtgcca ccctggccga
      301 ccgcgaaaac ccgttccggg gacactgcca ccgcgatgag atccttgccg gtgatcggat
      361 tgagcgccgt tgccgtcgtg ccgtcggccc ggacgaccat gccccgcttg cccaccgcga
      421 ccattccacc ctggccggca gccattgcgc tgagttcgac aggcacgtcg agcgtcagtt
      481 cccgccagcc gccgtccgat gacttcgagt ggatcgtttt tccatccgaa acccagactg
      541 ttccgtcatc gccgcgcgca atcaatgaca gatccatcag ccgggtgccg attctttccg
      601 gcacctgttc gtgagtgacc aggtcaaacg accagacaag cgacccaccg gcggcaaaaa
      661 gcacctttcg gtcgggttcg aaggcacatg cccggatgtc gcctccaccc acaagcggaa
      721 gcgcgcggat gccgcccagg ttgtcgattt cccatgcgtt tccacgggcg tcgacaacta
      781 ttccggattc gttcccgtaa catgccgcca tcgcatcaag gcggtacttc agggaaaggc
      841 tttcggctga aagccggttg acggcggtca caggcgggtt ttccgggacg aaccgcatcg
      901 tttgaaccgt cgagtacgga tccgaattgg aaaccggtga cgccgcctcg gtcctgacaa
      961 gaaattccga gtcggccaac tggtcgccca tttcgcgcgg aagtcctgtg aacgtgaaga
     1021 cgcggccgtc gggaatcgat atcgggtgcc acaggttcag gtacccgtcg gaaccgagcc
     1081 tgagcgagag cgtggctgag tggttgccgg ttccggaaga agaaccgggc gcggaaatca
     1141 tcgatgtcgt cagggtccga tccagcggga ttgacagacg gacctcgttt ccggaactga
     1201 tcgccgacga cgtgacctgc acgtgacgtt tcacgcccag cagcgtcggg acgaatttcc
     1261 cgtcgaccct gttcctgtaa ccgccgacgc atgtgacagc aacgtccccc agcctcgagt
     1321 tgatggcgaa gaatcccctg tcgtcgggca cggcgccagg tccgtacttc tccgcccagc
     1381 gggtcgtgct ggagtctgcc gtcgcgcatc tgatttcggt tctgcccttc agcggggtgc
     1441 agaagtgatg ttcgtcgccg gtggaaccgc aggtgtaaag atccggacaa tccatgctcg
     1501 tgtcgcatga cttcgagcat gtcagtgcgg tgatgccgga catcacgcag aagtatccct
     1561 gtccgcaatc ctcgtctgtc cggcatggtc tgcacagcgc cccgtcggtg agaggaaccc
     1621 cggcgcagga agaatgcggt gcgcggaatt ccttgtcgat tcctgtgacc tggcccgtga
     1681 ttattccggg gttcggggga aagaccgttt cgccgtcacc cccgtttccc gtgtccggga
     1741 tttcccggat caggggttcg atgaagaagg taatgttctc cgcgctggtg ccgaccaggc
     1801 tgttggtcgt gtagtcaatc cgggatgcgg tcgccatgac cggcccggtc aggccccttg
     1861 tcgaaagggt tgccatgccc ctggcgtccg tgaagccttt cagcggcgtc cacggatcct
     1921 ggccaagcat gacgaatgca ccaggaaccg gcttgtcggt atatacgtca agcaccgtga
     1981 tgttgaccgc gccggcgatc gggcgccccc acgcgccgcc caggaagctg gtcggatcga
     2041 agtaattgaa ggggtatgaa agcctttcac ggattccgtc gggagtgacc gcgaaaactt
     2101 cggccggccc ggcttcgtcg gcaggtggag tcctgatggc gattctgccg ggatcggtgc
     2161 cgtcaaggat ttcggcctcg gcgcccccga tgaaaatccg ggttccaggc ctgagtcccc
     2221 ggccggtcac ccatgcgaaa gtgccgcccg ccagggatcc gcgatccggg ctgaccgcca
     2281 gtattgaagc gcgccgggcc gagcacgtga gagccccctt cgagacgatc tgccgtccgt
     2341 cggcaagcag taccgaaacg tcgaacacgc cggcttccag gtcgggcagc tcgaaacgga
     2401 tttcgtcgtc tgaaggcggg ccggtcatga ccgccagaac cggcccgatg ccgacctgaa
     2461 cggcgcctgc caggccggag cccagaatcg acccggccgt gtcgccaccg ggaatgcatt
     2521 cagcaggatt aacggaagaa atggatgcgg gggcaaggaa ggtgaatgcc ttcgatgcga
     2581 tggctgttcc ggacggcgtc tgcaaggcga catcgacggt gcctggactt ccctggggaa
     2641 tgatgaagcg aatcgtcgtg gcatcgggaa tcgacgtgac cacggccggg gtgtccccga
     2701 gggtcaggga gatccgggca ggttcatcca cgtccagtcc cgtgacgtga atggttccga
     2761 cgattccgga ggttgcgtcg gccgctttcg gaatcaccga acggattttc aggccgcccg
     2821 attgaagatc cgccggatcc agcgccacaa ggcacgacgg cagttcggtc accccccacg
     2881 gacctccgac ggttacatcg acggagccgg gcgccgcgtc gattccaaga aatgcccgta
     2941 tttcaagccc cccgtcgata acggatgacc ccaggacgga accttgcgac agaaggattg
     3001 agtcggccgc gtcgagcccg gagccctcca cgcggaacgt tacgtttccg tcgccgtctg
     3061 atccaaggcg actgatcacg cccgggctgc atcccgatac cctgggctcg gaaacgtatg
     3121 aaaaaccgcc gggcagccgc cccgaagcgc cacccgaaat cactagaacc tcgaccggtc
     3181 ccgggtcgcc gtgcggcgtc acggcaagga ctgtcgaatc gtccagccgg tgaaccttca
     3241 acgcgtggcg tccgtcgatt atcacgttgc agtccggcgc gaagccatga ccggtgataa
     3301 tgaccggggt gccgcccgaa accggcccgg acggcggcga tattgaagcg acggccgtat
     3361 ccgcctgata catgaatcca ggtctcagaa ccgttttctt tgcgtcaggc cggacgatga
     3421 agacgtcaac cgtctcggag tacccgcttc cgccgggcgt ggaagttctg atggaggtat
     3481 cgctttcaac aatggtttcg acaccttcga cggtaccgaa gaacaccctg gatcccaggt
     3541 cgaaaccgcg tccctcaata tgaaccctgg ttccaccgga tgccggcccg gatgacggct
     3601 ccatgcgttc gatgatgaaa gccgaagtcc cgtcgtcgtt tccggcccag tcgtccggcg
     3661 ggtcgaacgg gttgtcgtgc agcgtgatgt cagggatgtc gaagtccggg taaaacgaag
     3721 agatgtcgca accgggaagc acggcggtca acaggacaag gctcgccacc aggccggccg
     3781 tttccggaaa gtgtgtccat tcgtgacggt ttgccggccg cttcaagtcg cctccgggtc
     3841 agcggaaggt gagtcgcgta cattatcgga actgacggcc cgccggtcaa acaacttcgg
     3901 cttgtgcgag ctgttgggcc
//
LOCUS       DAOPPA010000308         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25209_length_3905_cov_3.890390, whole genome shotgun sequence.
ACCESSION   DAOPPA010000308 DAOPPA010000000
VERSION     DAOPPA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25209_length_3905_cov_3.890390"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PKH54_09615"
     CDS             complement(<1..1586)
                     /locus_tag="PKH54_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00194.1"
                     /translation="MRAFLATAFLAAIFPAAAAAIPAEATFNIVVEAHCVSNATVCGY
                     GTEPLFERATRQSIFDLNNYWRPTGFSFRLKEINYHYGDDYSEVTGPENTATAPDNLT
                     KLNELRSKASKNPSDVYWFVLDSMTGGCFSGVPGTHMGRTPDPKAPDEYYGVFCTGVD
                     GNTMAHEIGHHFCLAHPFSHQDPASPAAGVNHDGDGIADTPEDPGEFEYCDVNGTQDK
                     KAEVDGVFPVNTNCVADAESCATQINDNRDWCGYSQVAADAGSFMPFHCTSDCLRKSG
                     GTISSINYSPTMSLTMSYYFRPCGGPYVYLGQAHEGFSPGEVAVMRSCYFDGNERGQL
                     VNRCAGDTDTDWDGICDKKDPCPANRASVWDADSDNDGIPDVCDSCKFVDNLGIDTDG
                     DGVDNACDPNDDNDACPDDIDDNPTSATMQIGTVQMPGCNTTTHPVIVSESGNYDNDD
                     ELDCQDDDDDNDGIPDTEDPCPHLADSELCIIPGQGCALETPWEECMGGGCQFDLLAE
                     LESLRNPDDPRQRFGAVIMD"
     gene            complement(1681..2505)
                     /locus_tag="PKH54_09620"
     CDS             complement(1681..2505)
                     /locus_tag="PKH54_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOD00195.1"
                     /translation="MPVSDKKVAIVTGASAGIGKSVVRTFLADGWTVYGLARRVPQMQ
                     DILSEGAKVIGVDLTDDAATTAAVQQILDAEGRIDALINNAGYGSHGPVELVPTDEAR
                     RQFEVNVFAVTRLSQLVIPAMRSAGRGTIVNIGSIAGRMWMPFGGWYHATKYSLECLS
                     DAMRLELAPFGIRVVLVQPGAIATEWGGIAVNNLLANSRGTPYEKPASAFSGILAGEG
                     MAVGPHRVAAVVRRAVNARHPCRRYATPFHAKAIIFMHWLLPTAVWDFALRLMLRR"
     gene            2730..>3905
                     /locus_tag="PKH54_09625"
     CDS             2730..>3905
                     /locus_tag="PKH54_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00196.1"
                     /translation="MKRVLAGATIACVIGLMSPVAGLAQDDQEPRPVVAVMDVLTQGL
                     KISDGTRGMLTRYMGIKIAMGGVFEVAPYEEMHKVISDLKLKSAADCYDQACRIRLGQ
                     EMAASKNVNTTIWKLDSQCSVIVELLDLRKATAEHAVEVAGLKCGDADLKAGVEKAAE
                     RLSREYGQGRGTSGTYDLKVQVQEKPAAAPGTMTFVDVDGDAYVGLTKVRKPADKLEL
                     SPGMYMVTVDGYEFKRFSEAFEVKPGLLTTVKPLKAALGSDDKAAWRARFKSLPDWVM
                     GKAERPDAVDEKLGEQLRGLGSARFINPLQGEIDAVSRAVVDLARQWETYRRARIKED
                     IAAGKTVEGTEASDEEQEVVDAASSITKVPELKVSILHPAAAFGDTMFVGVGMVREGR
                     "
BASE COUNT          768 a         1048 c         1278 g          811 t
ORIGIN      
        1 tcgtccatga tgacggcgcc gaatctctgc cttggatcgt ctggattccg cagggactcc
       61 agttccgcca gcaggtcaaa ctggcatccc ccgcccatgc actcttccca gggggtttca
      121 agcgcgcagc cttgtccagg aattatgcac aactcgctgt cggcaaggtg cgggcacgga
      181 tcctcggtgt ctggaatgcc gtcattgtcg tcgtcgtcgt cctggcaatc caattcgtcg
      241 tcattgtcgt aattgccgct ttcggaaacg atgacggggt gggtcgtggt gttgcatccg
      301 ggcatctgaa ccgttccgat ttgcatcgtt gcactggtcg gattatcgtc gatgtcatcc
      361 gggcatgcgt cgttgtcgtc gttcgggtcg catgcgttgt caacgccatc accgtccgta
      421 tcgataccca ggttgtcaac gaatttgcac gaatcgcaga cgtcggggat tccgtcgtta
      481 tctgagtcgg cgtcccatac cgaagcgcgg tttgccgggc atggatcctt cttgtcgcag
      541 attccatccc agtctgtatc cgtgtcgccg gcgcaccggt tgacaagctg gccgcgttcg
      601 ttgccgtcga agtaacatga gcgcatcacc gcgacttcac cggggctgaa gccttcgtgg
      661 gcctggccga gatagacgta cggcccaccg catggtcgga agtaataaga catcgtcagt
      721 gacatggttg gtgaatagtt gatcgaggaa atcgtcccgc cggacttgcg aagacagtcc
      781 gacgtacagt ggaacggcat gaaactgccg gcatcggcag cgacctgcga gtatccgcac
      841 cagtcgcggt tgtcattgat ctgggtcgcg caggattcgg catcggcgac acagtttgtg
      901 ttgaccggga aaaccccatc gacctccgcc ttcttgtcct gtgttccgtt gacgtcgcag
      961 tattcgaatt cacccgggtc ttccggcgta tccgcaatcc catccccgtc gtggttgaca
     1021 ccggcggccg gcgatgccgg atcctggtga ctgaacggat gcgccaggca gaagtggtga
     1081 ccgatttcgt gcgccattgt gttgccgtca acgccggtgc agaaaactcc gtagtactca
     1141 tccggagcct tgggatccgg ggtgcgcccc atgtgagtgc ccgggacgcc ggaaaaacag
     1201 ccgccggtca tgctgtccag tacaaaccaa tagacgtccg acggattttt tgatgccttc
     1261 gaccgcagtt cgttcagttt ggtcaggtta tccggtgcag tcgcggtatt ctcaggaccg
     1321 gtaacttccg aataatcatc gccatagtgg taattgattt ccttcaggcg aaaagagaag
     1381 ccggtggggc gccaatagtt gttcaggtcg aaaatggatt gccgggtggc cctttcgaaa
     1441 agtggttccg tgccgtaccc gcaaacagtt gcattgctga cgcagtgtgc ctcgaccacg
     1501 atgttgaacg tggcctcggc cgggattgcg gccgcggccg cgggaaagat cgccgccagg
     1561 aaagctgtcg caagaaatgc cctcataatg ccccccaatg ttttcagaag cagccgtgat
     1621 tctacgatgt cgggcgatgg ttgtcacgcg tcagcgactg ccgcgagcct gggtggctgg
     1681 tcagcggcgt aacatcaacc tcagcgcgaa gtcccagacg gcggtgggaa gcagccagtg
     1741 catgaatatg atcgcctttg cgtggaacgg ggtcgcgtaa cgtcggcatg ggtgacgggc
     1801 attgaccgcg cgccgaacaa cggcggcgac gcgatgcgga ccgaccgcca tgccttctcc
     1861 ggcgaggatg ccggaaaagg cggatgcagg cttttcgtaa ggcgtgcccc gggaatttgc
     1921 cagcaggttg ttgaccgcga tgccgcccca ttcagtggcg attgcgccag gctgaaccag
     1981 gacgacgcgg atcccgaagg gggcgagttc cagccgcatt gcgtccgaaa ggcactcaag
     2041 cgagtacttt gtcgcgtggt accagccgcc aaaaggcatc cacatgcggc ccgcgatcga
     2101 gccgatgtta acgatcgtgc cacggccagc tgaacgcatc gccgggatca caagttggga
     2161 caggcgggtg acggcgaaga cgttgacttc gaactggcgg cgcgcctcgt cggtgggaac
     2221 cagttcgacc ggcccgtggc ttccgtagcc ggcgttattg atcaacgcgt cgatgcggcc
     2281 ctccgcatcc aggatttgct gaacagccgc ggttgtggcc gcgtcgtcgg tcaaatcgac
     2341 tccgatgacc tttgcgcctt cggacagaat gtcctgcatc tggggaacgc ggcgggccag
     2401 gccgtagacg gtccagccgt cagccaggaa cgtgcgtacg actgacttgc cgattccggc
     2461 gctggcaccg gttacgattg cgaccttctt gtcggacact ggcatattaa cctcctggac
     2521 gtgtcggcgt cgccgatttg gcgaatgggg cacgattcat ggcggattat atccggaagc
     2581 cgaaccgtcg cggcatcgtg ttcatcggat ttcgcattat ccgggcgagt cggtgttgcg
     2641 aagcgggtgg acggggttga taatcaccgg gacggattct tgattggctt gctttcgctg
     2701 gtgggaagcg cttgaacggg tgtcatttta tgaagagagt gttggccggg gcaacgatcg
     2761 cttgtgttat cgggctgatg tcaccggttg cgggactggc gcaggacgac caggagccca
     2821 ggccggtcgt ggccgtgatg gatgttttga cgcagggcct gaaaatttcg gacgggacgc
     2881 gcggcatgct gaccaggtac atgggcatca agattgccat gggcggcgtg ttcgaggtcg
     2941 ccccatatga agaaatgcac aaggtgataa gcgacctgaa gctgaagtcg gcggccgact
     3001 gttatgacca ggcgtgcagg atcaggctgg gccaggagat ggcagcctcg aagaacgtga
     3061 acacaacgat atggaagctg gattcgcagt gctcggtgat tgtcgagttg ctggatttgc
     3121 ggaaggccac cgccgagcat gcggtcgagg tggccgggtt gaaatgcggt gatgccgatt
     3181 tgaaggcggg ggttgaaaag gccgcggagc ggttgagcag ggagtatggg cagggccgcg
     3241 gaacctctgg aacgtacgat ttgaaagttc aggttcagga aaagcctgcg gcagcgccgg
     3301 gaaccatgac atttgtggat gtggatggag acgcttacgt cggacttaca aaggtcagga
     3361 agcccgcgga caagcttgaa ctgtcgcccg gcatgtacat ggtgacggtc gatggatatg
     3421 agttcaaacg gttttcggaa gcattcgagg tcaagcccgg gctgctgacg acggtcaagc
     3481 ccctgaaggc cgcgcttggt tcggatgaca aggcggcgtg gcgggctcgt ttcaagtcat
     3541 tgccggactg ggtcatgggc aaggccgaaa ggccggatgc cgtcgatgaa aagcttggcg
     3601 aacaactgcg cgggttgggc agtgcccggt tcatcaatcc gcttcaaggg gaaatcgacg
     3661 cggtttcgcg ggccgtggtc gatctcgcaa ggcagtggga gacatacagg cgggcacgaa
     3721 taaaggaaga cattgccgcc ggtaagaccg tcgagggcac ggaggcatcg gacgaggagc
     3781 aggaggttgt cgatgccgca tcttcaatca ccaaggttcc cgaactgaaa gtttcaatcc
     3841 tgcatcccgc ggcggccttc ggcgatacca tgtttgtcgg cgtcgggatg gtacgcgaag
     3901 gtcgt
//
LOCUS       DAOPPA010000309         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25272_length_3897_cov_3.090838, whole genome shotgun sequence.
ACCESSION   DAOPPA010000309 DAOPPA010000000
VERSION     DAOPPA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25272_length_3897_cov_3.090838"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKH54_09630"
     CDS             <1..1213
                     /locus_tag="PKH54_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOD00197.1"
                     /translation="PHGARAASAGFILLMTSDVAAMSQGTRTGAAHPIMAIGGLFPIG
                     DQQQETESEPAAETAPPADPSAVEPTGVPAQSAPGEPAGAERVTPVTPPAPTRAPDPA
                     KMGSVLMEKVGNDMKAFIRSITEARGRNPSQAVLAVSVSKSWSESEALKAGLIDVVAS
                     DEADLLKQLQGRQVKRIDGNVVTLNPDGATIVEVEMTGRERILRHVVDPNVAFILLLL
                     GILLIYIEVTHFGLIIPGVTGLACLAVSVSGLSLLPLNSAGVLMILAGIGLLIAEAFV
                     TSYGLLGLLGVVTMAIGGIILVDLPRIEGIALDPVIAISAAVGFGLIVLVLVKLVVKA
                     VRARPSTGAESLVGREAVAVRTFQGRGSVMVHGEYWSASCDTLVAKGATVRIIAVDGL
                     NLVVEPVETIS"
     gene            1226..1993
                     /locus_tag="PKH54_09635"
     CDS             1226..1993
                     /locus_tag="PKH54_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOD00198.1"
                     /translation="MSGEIVEFLMKGPVFIIVILVVYFLSSIKVLREYERGVVFRLGR
                     LLDKPKGPGLIFVFWPIDKIIKVDLRTVTMDVPSQDIITKDNVSCTVNAVAYFRVVDA
                     QRAIVDVEHYMYATSQLAQTTLRSVLGQVELDELLSEREKLNIELQSILDRHTDPWGI
                     KVSLVEMKQVDLPDEMRRALAKQAEAERLKRAKIIHADGEFQSAQKLAEAAEVLATQP
                     NTIQLRYLQTLSDIGMNNSKTIVFPFPTELLQMVKPK"
     gene            complement(2020..2685)
                     /locus_tag="PKH54_09640"
     CDS             complement(2020..2685)
                     /locus_tag="PKH54_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD00199.1"
                     /translation="MEPQDTTPVADRISTLRHDVMNPLTVVLCYSKLLLGREDIPARA
                     REYVKMIVSEAERCVQIFEDDKNAHSVMQPEPQDSPKSTPTALSLDQVVNILVVEDDE
                     GIRMLASEVLVTGFLDRGVRAVVDCVPDRAAALIRVRERNYHAMVIDLNIDSPSGGID
                     LLAGIDAHRPGAANRTVMVSGGVLDTMTQKMLDRMGIPLLEKPFSINELVRMVQNIIN
                     RNH"
     gene            complement(2759..3679)
                     /locus_tag="PKH54_09645"
     CDS             complement(2759..3679)
                     /locus_tag="PKH54_09645"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOD00200.1"
                     /translation="MNTIIDQAIDSFLFDLRVRRGLSRNTIEAYGRTMRRFSGWVMKS
                     FGSEMNPAELDRNQIESFVRELREGGLGDRSLSAAVVAVRQFYVWFCADRRLDVNPME
                     DFEVPRFSAALPVVFDEMDVAAVLQAPAGTPQGIRDLAILELLYGSGLRISEVLGLGV
                     DDVNTIANFVIARGKGRKERVVPISEPCARAVSEYLQTARPVLLKGRRVSRPGVREPM
                     FVTARGTVLTRQGFFKNLQQYGLKANLVRAISPHKLRHSFATHLVENGADLRSVQEML
                     GHADISTTEIYTHVSRAHLKKVYRKAHPRA"
     gene            complement(3676..>3897)
                     /locus_tag="PKH54_09650"
     CDS             complement(3676..>3897)
                     /locus_tag="PKH54_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00201.1"
                     /translation="IFALRSRLWAAAADNVETRRVAVRNLAASAEWSQRAGLDYPACD
                     LLLAALELNPRDRAIRARLDGLGGGRCAQ"
BASE COUNT          804 a         1186 c         1088 g          819 t
ORIGIN      
        1 gccgcacggc gcccgggccg cgtcggccgg cttcatcctc ctgatgacgt ccgacgtcgc
       61 ggcgatgtcc cagggaaccc gcaccggcgc tgcgcatccg ataatggcca tcggcggcct
      121 ttttcccatc ggtgaccagc agcaggaaac cgaatctgaa ccagccgcgg aaaccgcgcc
      181 accggcggat ccgtcggccg ttgaaccgac gggtgtgccg gcccagtcag cgcccgggga
      241 gccggccggg gccgaacgtg tcacgccagt caccccgccc gcgccgacac gcgcgcctga
      301 tcccgcgaaa atgggctctg tcctgatgga aaaggtcggc aacgacatga aggcattcat
      361 ccgatccatc accgaggccc gcggccgcaa tccgtcccag gcggttcttg ccgtcagcgt
      421 cagcaagtcc tggtcggagt ccgaggcgct gaaggcggga ctgattgacg tggtcgcaag
      481 tgacgaggcc gaccttctga agcagcttca gggacgacag gtcaagcgca ttgacggtaa
      541 cgttgtcacg ttgaaccccg atggcgcgac aatcgtcgag gtcgagatga ccggtcggga
      601 acgaatactg cgccacgtgg tggatccgaa cgtcgccttc atacttcttc tgcttggcat
      661 tctgctgatc tatatcgaag tgacgcattt cggtctgatc attcccggcg tcaccggttt
      721 ggcttgcctt gccgtgtcgg tatccggtct gtcgcttctt ccattgaatt cggcgggcgt
      781 gctgatgatt ctcgccggca tcggcctgct gattgccgaa gcattcgtga ccagttacgg
      841 tcttcttggc cttcttggtg ttgtaaccat ggcaattggg ggcataatcc ttgttgatct
      901 tccgcgaatc gaggggattg ccttggatcc agtaatcgca atatcggccg ccgtcggttt
      961 cgggctcatc gtgctggttc ttgtcaaact ggttgtcaag gcggtcaggg cccgtccttc
     1021 caccggtgca gagagccttg tcgggcggga agctgttgcc gtccggactt tccagggtcg
     1081 aggcagcgtc atggttcacg gtgaatactg gtcggcgtcg tgcgacaccc tggttgccaa
     1141 aggcgccacg gttcgaatca ttgccgttga cgggctgaat cttgtggtgg aacctgttga
     1201 aacaatctct taaaaggggg ctttcatgtc tggcgaaatc gttgaattcc tgatgaaggg
     1261 accggtgttc atcatcgtca tcctggtcgt gtacttcctg tcttcaatca aggttctgcg
     1321 ggaatacgaa cgtggcgtcg tgttccggct gggccgtctt cttgacaagc ccaagggccc
     1381 cggcctgatt ttcgtattct ggccgatcga taagatcatc aaggttgacc tgcgaacggt
     1441 cacgatggac gtcccgtccc aggacatcat caccaaggac aacgtaagct gcaccgtcaa
     1501 cgcggtcgcc tatttccgcg tggtcgatgc ccagcgggcg atcgtcgatg tcgagcatta
     1561 catgtacgcg acatcacagc ttgcccagac aacgcttcgc agcgtgcttg gacaggtcga
     1621 actcgatgaa ctgctttcgg aacgcgagaa gctgaacatc gagcttcaga gcatcctgga
     1681 tcggcacacg gatccctggg gcatcaaggt ttcgctcgtg gaaatgaagc aggtggatct
     1741 tcccgatgaa atgcggcgcg ctcttgcgaa gcaggccgag gccgaacgac tcaagcgcgc
     1801 caagatcatt catgccgacg gcgagttcca gtccgcccag aagcttgccg aggcagccga
     1861 agtcctggcg acccagccga atacgatcca gcttcgttac ctgcaaacgc tgtccgacat
     1921 cggcatgaac aacagcaaga ccatcgtctt cccgttcccg accgagcttc ttcagatggt
     1981 taagccgaag taggactgcc tttcccgccc gttaatcact caatgatttc gattgattat
     2041 attctgaacc attctcacca gttcgttgat tgagaatggt ttttcaagaa gcgggattcc
     2101 catccgatcc agcatcttct gagtcatcgt gtccagcacg ccgcccgaaa ccattaccgt
     2161 acggttcgcg gcgccgggac gatgggcatc gatcccggca agcagatcga ttccgcctga
     2221 cggactgtcg atgttcagat cgatgaccat tgcgtggtaa ttcctttccc tgacccgaat
     2281 cagcgcggcc gcgcgatccg gaacgcaatc gacgacggcc ctgacgcccc tgtcgagaaa
     2341 tccggtcaca agaacctcgc tggccagcat gcgaattccc tcgtcatcct cgacgacaag
     2401 aatgttcacc acctggtcca aagacaacgc agtgggcgtt gattttggtg aatcctgcgg
     2461 ttcgggttgc ataaccgagt gcgcattctt gtcgtcttca aaaatctgca cgcaccgttc
     2521 ggcctcgctt acgatcatct tcacgtactc gcgggccctg gccgggatat cctcccgccc
     2581 aagcagaagt tttgaatagc acagcacaac ggtaagcgga ttcatgacgt cgtgccgtaa
     2641 tgtgctgatg cgatcggcaa ctggcgtggt gtcttgcggt tccatccggc acacgattca
     2701 tagaattggc cggactatat cacaacttcc ggcagtacaa gcgaaatgat aaagaatatc
     2761 aggccctggg atgcgccttg cggtaaacct ttttcagatg ggccctgctt acatgagtat
     2821 atatctccgt ggtcgatatg tcggcatggc ccaacatctc ctgcacggaa cgaagatcgg
     2881 cgccgttttc aaccaggtga gtcgcgaatg aatgtcgcaa tttgtgcggc gagatcgccc
     2941 gcaccaggtt ggccttcaag ccatactgct gaagattctt gaaaaatccc tgacgggtca
     3001 gcaccgtccc gcgtgcggtt acgaacatcg gctcacgcac gccaggccgg gaaacgcgtc
     3061 gccccttcaa aagaacgggc cttgccgtct ggagatattc ggaaacggcg cgggcacagg
     3121 gttccgagat cggcaccacc ctctcctttc tgcccttgcc ccttgcgata acaaaattgg
     3181 cgattgtgtt gacgtcatcg actccgagtc cgagaacctc gctgattcga agaccgcttc
     3241 cgtaaagcag ctcaagaatc gcaagatccc gtattccctg gggagtcccg gccggggcct
     3301 gcaagacggc cgcgacgtcc atttcgtcaa agaccaccgg aagcgcggcg gaaaagcggg
     3361 gaacctcgaa atcctccatc gggttcacat caagtctccg atccgcgcag aaccacacat
     3421 agaactgcct caccgccacg accgcagccg aaagacttcg gtcgccaagg ccaccctcgc
     3481 gaagttcccg cacgaacgac tcaatctggt tcctgtcgag ttcggcggga ttcatttccg
     3541 atccgaacga cttcatcacc catccggaaa agcgacgcat cgtccgtccg tacgcctcaa
     3601 tcgtgttccg ggaaagcccc cgccttaccc tcaggtcaaa aaggaacgaa tcaattgcct
     3661 gatcgatgat ggtattcact gtgcacaccg cccgccacca aggccgtcaa gccttgccct
     3721 gatcgccctg tcccgcgggt tcagttcaag cgcggccagc agaaggtcgc atgcggggta
     3781 atccagcccg gcgcgctggc tccactcggc tgaagccgca agatttcgaa cggccacccg
     3841 ccgagtttcg acgttgtccg cggcggccgc ccacagccgg ctgcgcaagg caaaaat
//
LOCUS       DAOPPA010000310         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25338_length_3888_cov_4.430733, whole genome shotgun sequence.
ACCESSION   DAOPPA010000310 DAOPPA010000000
VERSION     DAOPPA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25338_length_3888_cov_4.430733"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3645
                     /gene="rpoB"
                     /locus_tag="PKH54_09655"
     CDS             <1..3645
                     /gene="rpoB"
                     /locus_tag="PKH54_09655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOD00202.1"
                     /translation="FENDKGRTHASGKLLYSARIIPNRGSWVDFEFDHKDVLWVRIDR
                     RRKIHATVLLKALGFSSWEILDRFYSTFVLRMTADGKFFRKVDPAQMEGQLSKVDIVD
                     PATGKMIVRKGEKLGRMRLKKVVEAGVTELLWSDDAIVGSVFGDDIVDEETGEIIFEC
                     NEEVTEKAISKLRDSAIEREIRILHIDGVTAGPYLRNTLLLDKVESPLEACVEIYRRQ
                     RPGDPTSPEQARAYFNSLFFSSARYDLSSVGRLKMNFKFGQNVPLDQTTLLKDDFFNV
                     VQYLLDLRMSKPGVRVDDIDQLSNRRVRAVGELMENQFRIGMVRMERAIREKMTMSPD
                     LATMMPHDLVNAKPVSAVLKEYFGASQLSQFMDQTNPLSEITNKRRLSALGPGGLTRD
                     RAGFEVRDVHATHYGRVCPIETPEGPNIGLISSLSTYARVNDFGFIETPYRRVVDRKV
                     TDEVIYMTAMEEEEQTIAQADVEVDADGYLIGDLVQARRNEEYPAVPPSEVTLIDVSP
                     AQLVSVAAALVPFLEHDDANRALMGANMMRQGVPLVRAEAPIVGTGLEWVVARDSGVL
                     TYARHEGVVESVDANRIVVRRTGDDIDGETLTPDIYSLTKYRRSNQNTCINQKPLVRK
                     GQKIAKGQVLGDGAATQQGELALGQNVVVAFMPWGGYNFEDSILMSERMVQQDFFTSI
                     HIEEFECVSRDTKLGREEITQDIPNVSEEALSNLDASGIVRIGAEVTTGDILVGKITP
                     KGETQLSPEEKLLRAIFGEKAADVKDSSLRVPPGVTGIVVGAKVFSRKGAEKDDRTRE
                     MEDLEEAVMLKDRDDELRVMTRAMRERLVAILEGRTVAAPVSNSAGDVIIKKNSKLKA
                     ADMANLSIDVLGHIRLEDEATERRVAQLIEVMNRQKEIISVKFAEKISRMKRGDELPP
                     GVIKTIKVYVAIKRKLQPGDKLAGRHGNKGVLSRILPAEDMPYLADGRPVDVVLNPLG
                     VPSRMNVGQILESHLGWAAREMSSKIGELVEKGGVKGLREKFGKLFTTSTMKKILDEI
                     DDSEMIDFARQFTTTGIPVGSPVFDGAEEREIKLWLEEAGLATHGKTTLFDGRTGEPF
                     DSDVTVGVMYMMKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRIGEMEVWAFE
                     AYGAAYALQEVLTVKSDDVAGRTATYDSIVKGEYNVHAGLPEVFNVLLKELKSLAIDA
                     QLIEADQAQA"
     gene            3699..>3888
                     /locus_tag="PKH54_09660"
     CDS             3699..>3888
                     /locus_tag="PKH54_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00203.1"
                     /translation="MFTGFERPRDPLSIEGMRLALASPKTILGWSYGEVKKPETINYR
                     TFKPERDGLFCAKIFGPVK"
BASE COUNT          829 a         1161 c         1191 g          707 t
ORIGIN      
        1 ttcgagaacg acaagggccg cacgcacgca tccggcaagc tgctgtattc cgcccgaatc
       61 atcccgaacc gtggctcctg ggtcgatttc gaattcgacc acaaggacgt cctctgggtc
      121 aggatcgaca ggcgtcgcaa gattcacgca accgtcctgc tgaaggccct cggcttctcg
      181 tcctgggaga tcctcgatcg gttctacagc acgttcgtcc tcaggatgac ggccgacggc
      241 aagttcttcc ggaaggtcga tccggcccag atggaaggac agctttcgaa ggtcgatatc
      301 gtcgatcccg ccacaggcaa gatgatcgtc cgcaagggtg aaaagcttgg ccgcatgcgc
      361 ctgaagaagg tcgtggaagc cggcgtgacc gagcttctgt ggtcggacga cgccattgtc
      421 ggatccgtct tcggcgacga catcgtcgat gaggaaaccg gcgagatcat tttcgagtgc
      481 aacgaggaag tgactgaaaa ggccatttcc aagcttcgcg actcggcgat tgagcgggaa
      541 atccggatcc tccacattga tggagtcacc gcgggaccgt atcttcgcaa cacgctgctg
      601 ctggacaagg tcgaatcccc gctcgaggcg tgcgtcgaaa tctaccgccg ccagcgcccg
      661 ggcgacccga caagcccgga acaggcccgc gcgtacttca acagcctgtt cttcagcagc
      721 gccaggtacg acctttcatc ggtcggccgc ctcaagatga atttcaagtt cgggcagaac
      781 gtgcccctgg atcagacgac gcttctgaag gatgacttct tcaacgttgt ccagtatctg
      841 ctcgaccttc gcatgagcaa gcccggcgtc agggttgacg acatcgacca gctttccaac
      901 cggcgcgtcc gtgcggtcgg cgaactgatg gaaaatcagt tccgtatcgg aatggtccgc
      961 atggaaaggg ccatccgcga aaagatgacg atgtctcccg acctggcgac gatgatgccg
     1021 cacgacctgg tcaacgcgaa gccggtttcg gcggtcctga aggaatactt cggcgcctcc
     1081 cagctttcgc agttcatgga tcagacgaat ccactttccg aaatcacgaa caagcgccgg
     1141 ctttccgcac ttggacctgg cggtctcacc cgcgacaggg ccggcttcga ggttcgcgac
     1201 gttcacgcga cccactacgg ccgcgtatgc ccgatcgaga caccggaagg tccgaacatc
     1261 ggccttatct cgtcgctttc gacttacgcc cgcgtaaacg atttcggttt catcgaaacc
     1321 ccttaccgca gggtcgttga ccgcaaggtg accgacgagg tcatctacat gacggcgatg
     1381 gaagaggaag agcagacgat cgcccaggcg gacgttgagg tcgatgccga cggatacctg
     1441 attggcgacc tggttcaggc ccgccgcaac gaggagtatc cggcggttcc gccgtccgag
     1501 gtgacgctga ttgacgtttc gccggcacag ctggtgtcgg tcgcggccgc cctggttccg
     1561 ttccttgagc acgacgacgc caaccgcgcg ttgatgggcg cgaacatgat gcgccagggc
     1621 gttccgcttg ttcgcgcgga agccccgatt gtcggcaccg gcctggaatg ggtggttgcc
     1681 agggattccg gcgtccttac gtacgccagg catgaaggcg tggtcgagtc tgttgacgcg
     1741 aaccgcatag ttgtccgccg caccggcgat gacatcgacg gcgagacgct gacaccggat
     1801 atctactcgc tcaccaagta ccgccgcagc aaccagaaca cctgcatcaa ccagaagccg
     1861 ctggtccgca agggccagaa gatcgcgaag gggcaggtgc ttggcgatgg cgcggccacc
     1921 cagcagggcg aacttgccct gggccagaac gtcgtcgtgg cgttcatgcc gtggggcgga
     1981 tacaactttg aagactccat cctgatgtcc gaaaggatgg ttcagcagga cttcttcacg
     2041 tcgatccaca tcgaggaatt cgagtgcgtt tcgcgcgata ccaagcttgg tcgcgaggaa
     2101 atcacgcagg atatcccgaa tgtttccgaa gaggctcttt caaacctgga cgccagcggc
     2161 atcgtccgca tcggcgccga ggtcacgaca ggcgacatcc ttgtcggaaa gatcaccccg
     2221 aagggcgaaa cccagctctc ccctgaagag aagctgcttc gcgcaatctt cggcgaaaag
     2281 gccgccgacg ttaaggattc ttccctccgc gttccgccgg gagtcacagg catcgtggtc
     2341 ggggcaaagg ttttctcgcg caagggcgcc gagaaggacg accgaacccg cgagatggaa
     2401 gacctggaag aagccgtcat gctgaaggat cgggacgatg aacttcgcgt gatgacgcgt
     2461 gccatgcgcg aacgccttgt cgccattctg gaaggccgca cggtcgcggc tccggtgtcg
     2521 aactccgccg gcgacgtgat catcaagaag aactcgaagt tgaaggccgc cgacatggcc
     2581 aacctttcca tcgacgtgct cggccacatc aggctcgagg acgaggcgac cgaaaggcgg
     2641 gtggcccagc tcatcgaagt gatgaaccgc cagaaggaaa tcatcagcgt caagttcgcc
     2701 gagaagattt ccaggatgaa gcgcggcgac gaactgcccc cgggcgtcat caagaccatc
     2761 aaggtttatg tcgccatcaa gcgtaagctc cagcccggcg acaagctggc cggtcgccat
     2821 ggtaacaagg gcgtcctgtc ccgcatcctg ccggccgagg atatgcctta cctggccgac
     2881 ggccgtccgg tcgatgtcgt gctgaatccg ctcggcgttc cttcgcgaat gaacgtcggc
     2941 cagatcctgg agtcgcacct gggatgggcc gcgcgcgaga tgagctcgaa gattggcgaa
     3001 ctggtggaga agggtggcgt gaagggcctg cgcgaaaagt tcggcaagct gttcaccaca
     3061 tccacgatga agaagatcct ggacgaaatc gacgattccg aaatgatcga cttcgccagg
     3121 cagttcacca ccaccggaat cccggtcggt tcgccggtat tcgacggcgc cgaggaaagg
     3181 gaaatcaagc tctggctcga agaggccggg cttgcaacgc acggcaagac gacgctgttc
     3241 gatggccgca ccggcgagcc cttcgattcg gacgtcacgg tcggcgtcat gtacatgatg
     3301 aagctgcatc acctggttga tgaaaagatg cacgcccgtt ccaccggacc gtactcgctg
     3361 gtcacgcagc agcccctggg cggcaaggcc cagttcggcg gccagcgtat cggcgaaatg
     3421 gaagtgtggg cgtttgaggc gtacggcgcg gcatacgcgc ttcaggaagt gcttacggtc
     3481 aagtccgacg acgtggccgg ccgcacggca acctacgact caatcgtcaa gggcgagtac
     3541 aacgttcatg ccggtctgcc cgaggtcttc aacgtcctgc tgaaggaatt gaagtccctg
     3601 gcgatcgatg cccagcttat cgaggctgac caggcgcagg cgtgattcat acgcgccggg
     3661 attccggcgc gagcaatttg ggggtcaccg tgaaggatat gttcaccggt ttcgaaaggc
     3721 caagggaccc gctttcaatc gagggcatgc gtctagccct cgcatcgccg aaaaccatcc
     3781 ttggatggtc ttacggcgaa gtgaaaaagc cggaaaccat caattaccgt actttcaagc
     3841 ctgaacggga cgggctcttc tgcgcgaaga tcttcgggcc ggtcaagg
//
LOCUS       DAOPPA010000311         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25479_length_3872_cov_4.405030, whole genome shotgun sequence.
ACCESSION   DAOPPA010000311 DAOPPA010000000
VERSION     DAOPPA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25479_length_3872_cov_4.405030"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1002
                     /locus_tag="PKH54_09665"
     CDS             193..1002
                     /locus_tag="PKH54_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00204.1"
                     /translation="MSAGFRLFAVGLVCIFSAIGCQDHGSVRDLSAGPLMPRVGESVT
                     WCEDLENRDVLHHSAEEECAYKYLVNGIQYWAHYASVAFVAELNPDCEGDIQRFCLPW
                     ALGENCLEFCPVKIGRIKTTLFGNPDQIETLATFGFEVWESNGQSVNAHLLDGNYPAS
                     RWTIAPGANLIFAHRACYARRYVGYDGLFLVTGVYPVEGDIVFDVDDASTTLTYLASK
                     LRSLELELDEAEDYARRCSTNYPSEDFHNTADDGQSGFEEVLDPPTKLSDL"
     gene            1178..1273
                     /gene="ffs"
                     /locus_tag="PKH54_09670"
     ncRNA           1178..1273
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKH54_09670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1296..2900
                     /gene="dnaX"
                     /locus_tag="PKH54_09675"
     CDS             1296..2900
                     /gene="dnaX"
                     /locus_tag="PKH54_09675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD00205.1"
                     /translation="MSYQVIARKFRPQTFDDIVGQEHVTTTLKNSIASGRAGHAFLFT
                     GIRGVGKTTAARVLAKALNCVNGPTVSPCNVCDACREITAGNSLDVKEIDGASNRGID
                     SIRELREGVAFAATRDRNKIYIIDEVHMLTTEAFNALLKTLEEPPPHVVFIFATTELH
                     KVPATIASRCQVFEFKRISLQALIEHLGYIASREGIEADPEALRVIAREADGSLRDAC
                     SLLDQVISFAGNTIDTAAVSEILRTGSRGSLERLLGAVLQEDAANAFRELSDAVDRGL
                     PPRQVLRDVSRFLSECLRVRLLGPDAARETGLTEQEIGVMIELVSNRTPEHLAALLNL
                     FVTAADRVADSRSPDLLAQAAIVKAARMSAFSSVAGLVDRLEQVASGLPPAPLASPAS
                     PPPARPSLVQPVARPVEPVASTRPVPVAATAPSPAPAGQAAPVAQPDPPAGRRWGSSA
                     AARGSVAEAVARLNQAAGVSAAPAPAPVPAQPVAAEPARNPDSVQQAPAGQPASLADR
                     LMVNPVVNRTIGLFGGDVISVEEDGR"
     gene            2929..3537
                     /gene="recR"
                     /locus_tag="PKH54_09680"
     CDS             2929..3537
                     /gene="recR"
                     /locus_tag="PKH54_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD00206.1"
                     /translation="MNKGLPESIVRLVGELRKLPGVGEKTALRYVLHMLSGGMNRASD
                     LSAALVEVIDRVRPCSRCGFPAEGDLCGFCSDQTRDMRSVCVVESVGDLLAVERMGEF
                     HGVYHILGGSVSALRGITPEKLRIPEFARRVEQEGIREVIVATGADAEGETTALYISK
                     MLESRGLQVRVTRPATGIPMGSSIEYLDGVTLMRAIRARREL"
     gene            complement(3594..>3872)
                     /locus_tag="PKH54_09685"
     CDS             complement(3594..>3872)
                     /locus_tag="PKH54_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00207.1"
                     /translation="LVAPDVLIIDELGKGRATEWEISILDQLVSRRYNAGKTVIATTN
                     YDPMKATGEDRMPGLLSRIDERIYSRLCELCEFIEIQGDDYRRLDSRF"
BASE COUNT          716 a         1084 c         1189 g          883 t
ORIGIN      
        1 ttatatcgcc tatcgcgggc agaacgacaa tcactggttg aatgtaggta ggtgggatgc
       61 gaatttgcca ggctggtctg acaaacacac tttgggcctg tcgatttggg ctgggcctgc
      121 agtagttgtt gggccaagtg atgatgttcg tatctactac gttgctccgt aagccattat
      181 gcgaggtgtc ccatgtccgc tggatttcgc ttgtttgcag tcggattggt ttgcatattt
      241 tcggcaattg gttgccagga tcatggctcc gtcagggatt tgtcggccgg gcctctgatg
      301 ccccgggttg gggagtccgt aacctggtgt gaggatctgg agaacaggga cgttttgcac
      361 cattctgcag aagaagagtg tgcatacaaa tatctggtga acggtatcca atattgggcg
      421 cattatgcat cggtggcctt tgtcgccgaa ctcaatccag actgcgaagg cgacattcag
      481 cgtttttgct tgccatgggc gctgggagag aactgtctgg aattctgtcc ggtgaagatt
      541 ggtcgaatca agaccacgct tttcggaaat cccgaccaga ttgaaactct ggccacattc
      601 ggtttcgagg tgtgggagtc caacggacaa tcggttaatg ctcatcttct tgacggaaac
      661 tacccggctt cgcggtggac gattgcccct ggagccaact tgatttttgc tcacagagcg
      721 tgctatgcga ggagatacgt agggtatgat ggtctgttcc ttgtaacagg ggtctatcca
      781 gtcgaagggg acatcgtatt tgatgttgat gatgcgtcta ctactttaac ctatttagct
      841 agtaaacttc gttcgttgga acttgaattg gatgaggcag aggattacgc ccgacgttgc
      901 agcacgaact atccttcgga agatttccac aatacagcag atgatggaca atcagggttc
      961 gaggaggtgc tggatccacc aacaaaattg tctgatttat gaccagagat ctaggctgga
     1021 gtccctccat tgatcatttt ccgttgccca actgtgacgc ctgcccttca ctcgctgggt
     1081 ttctgacctc accggttggg cgctttcact ttcctttagc gtgatgcttc gcatggaatt
     1141 gcggttgctt cggtcggtcg gtcatcttat aatgcgttcg ccgggacggg gcggcgtgat
     1201 tatcgcgtaa ccccgccagg tccggaagga agcaacggta agtggtgttg cgggtgttcc
     1261 gtcccggcat ttctccctcc ttgcagaaaa tcgatatgtc gtatcaagtc atcgcaagga
     1321 agtttcggcc acagacgttt gacgacatcg ttggccagga acatgtcacg acgacgctga
     1381 agaactcgat tgcctccggc agggccggcc atgccttcct ttttaccggc atccgcggcg
     1441 tcggaaagac gacggcggcc cgcgtgctgg cgaaggcgct gaactgcgtc aacggcccga
     1501 ccgtcagtcc ctgcaacgtg tgcgacgcat gtcgtgaaat cacggccggt aattcgctgg
     1561 acgtaaagga aatcgacggc gcatcgaacc gcggcatcga cagcatcagg gaacttcggg
     1621 aaggtgtcgc ctttgcagcg acacgcgacc gcaacaagat ttacatcatc gacgaagtcc
     1681 acatgctgac gaccgaagcg ttcaacgcgc ttctgaagac gctcgaggag ccgccgccgc
     1741 atgtcgtgtt catttttgcg accaccgaac ttcacaaggt tccggcaacg attgcttcaa
     1801 ggtgccaggt ttttgaattc aagaggattt cgcttcaggc gctgatcgag caccttggat
     1861 atatcgcttc ccgcgagggt atcgaggccg accccgaggc cctgcgcgtt atcgcgcgcg
     1921 aagccgacgg cagcctgcgg gatgcatgtt cgttgcttga tcaggtgatt tcctttgcgg
     1981 gaaacacgat cgacacggcc gcggtttccg agatccttcg aaccggttcg cgcggcagcc
     2041 tggaaaggct gcttggtgcg gtgttgcagg aagacgccgc caatgcgttc agggaattgt
     2101 cggacgcggt cgatcgcggg cttccaccca ggcaggtgct tcgcgacgtt tcacgtttcc
     2161 tgtccgagtg tctgcgggtc aggcttcttg gtccggacgc ggcgcgggaa accggcctta
     2221 cggagcagga aatcggcgtg atgattgaac tggtgtccaa ccggacgccg gaacatctgg
     2281 ccgcgctttt gaatctgttc gtcactgccg ccgatcgggt cgccgattcc cgcagcccgg
     2341 acctgctggc ccaggccgca atcgtgaagg ccgccaggat gtccgcattt tcgtccgtgg
     2401 ccggactggt cgatcgcctg gagcaggtgg cttcgggttt gcccccggca ccactggcgt
     2461 ccccggcttc gccgccgcca gcccgccctt cactggtaca gcccgttgcg cgtccggtcg
     2521 agcctgtcgc atcaacgaga cccgttccgg ttgccgcgac ggccccatcc ccggcccctg
     2581 ccgggcaggc agcccccgtt gcccagccgg atccgccggc cggtcgccgc tggggttcca
     2641 gcgcggccgc ccgtgggtct gtggccgagg ccgtggccag gctgaaccag gccgccgggg
     2701 tttccgccgc gcccgcgccg gctcccgtcc ccgcccagcc agtggctgcg gagcctgccc
     2761 ggaacccgga ttcagtccag caggcccctg ccggtcagcc ggccagcctg gcggatcgct
     2821 tgatggttaa tccggttgtc aacaggacaa tcggcctttt cggcggcgac gtgatctctg
     2881 tcgaggaaga tggtcgctga ggccgttcgt ccgcgctggt gtcattcaat gaacaaaggt
     2941 cttccagaat cgatagtcag gcttgtcggc gaactccgca agcttcccgg ggtcggtgaa
     3001 aagacggcgc ttcgttatgt gctgcacatg ctttcgggcg gcatgaacag ggcttcggat
     3061 ctgtcggccg cgcttgttga agtgattgat cgcgtccgtc cgtgcagccg gtgcggattc
     3121 ccggccgagg gcgacctttg cggtttctgt tcggatcaga cgcgggacat gcgttccgtg
     3181 tgcgtggtcg agtcggttgg cgacctgctg gcagtcgaac ggatgggcga gtttcacggc
     3241 gtatatcaca tacttggcgg ctcggtttcc gcccttcgcg ggatcacgcc cgagaagctg
     3301 aggattcccg agttcgcgcg ccgggtcgag caggaaggca tccgcgaggt catagtggcg
     3361 accggtgcgg atgcggaagg cgagacaacg gcgctttaca tcagcaagat gctcgaaagc
     3421 cgtggacttc aggtcagggt cacgcggccg gcaaccggaa ttccgatggg ttcctcgatt
     3481 gagtaccttg acggcgtcac gttgatgagg gcgatcaggg cacgccgcga actctagacc
     3541 gcatttccac cgcgatttga acgttattct ttcgtgatat ggggaaccac gcttcagaag
     3601 cgggaatcca gccgacggta gtcgtcgccc tggatttcaa tgaattcaca cagttcacaa
     3661 agcctggagt aaattcgttc atcgatcctg gaaagcaggc ccggcatcct gtcctcgccg
     3721 gtggccttca tcggatcgta gttggtggtt gctatgacgg tcttgcccgc gttgtacctg
     3781 cggctgacaa gctgatccag gatggatatt tcccattccg tggcgcggcc ctttcccagt
     3841 tcatcgatta tcagcacgtc cggggccaca ag
//
LOCUS       DAOPPA010000312         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25717_length_3847_cov_3.827004, whole genome shotgun sequence.
ACCESSION   DAOPPA010000312 DAOPPA010000000
VERSION     DAOPPA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_3847_cov_3.827004"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKH54_09690"
     CDS             complement(<1..384)
                     /locus_tag="PKH54_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD00208.1"
                     /translation="MLRYLLEKEFKQFGRNKFLPRMVFMFPFVSLLVFPLVANFDVRD
                     MNLSIVDHDHSPMSRRLTQKASASGYFRVTQVADSYERALRGIEDDTADIILEIPSGF
                     ERDMVRQRSAAVMIAANTVNGTRGGL"
     gene            complement(387..1493)
                     /locus_tag="PKH54_09695"
     CDS             complement(387..1493)
                     /locus_tag="PKH54_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD00209.1"
                     /translation="MKQFFAFVAKEFRHIFRDRLTTAILLGLPILMLFLFGFAITTEV
                     KNTRLAVYAPAPDDATRAIVDRLAANDYFILSRTLSDPRQIQDVFARGEVGLAVVFSE
                     NFQEEMLHTGAAQVQLIADGTDPNTAKTLVNYAQNIIAGYQQELLRDASVPYLIIPEV
                     KLLYNPSMKGAYNFVPGVMGMILLLICAMMTSISIASEKETGTMEVLLVSPVRPLQII
                     LAKTVPYFVISVIDLATILLMAVYVLGVPIVGSLALLIGTALIYIFMSLALGILISSV
                     ANTQSTALLISGMAMMLPVIMLSGMIFPIETMPWPLQWLSQAVPAKWFIAAVRKIMIR
                     GLDFESVRKELAVLSGMAVFLIAASLKRHRTRLE"
     gene            complement(1495..2232)
                     /locus_tag="PKH54_09700"
     CDS             complement(1495..2232)
                     /locus_tag="PKH54_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD00210.1"
                     /translation="MKVITARELTKRFGNFTAVDAISFEVEQGEIFGFLGANGAGKTT
                     AMRILCGLSRPTSGVGTVAGFDVSRQPEMVKASIGYMSQRFALYDDLKVWENIRLYAG
                     IYGVPARRIPERTDEILTLLNFMDGRNAMVGSLPLGWKQKLAFSVAMVHNPRIVFLDE
                     PTGGVDPAARRQFWELIYQAAESGITIFVTTHYMDEAEYCDRVSILVDGRIQALDSPR
                     ELKRQHEARTMNEVFFSLARRAERGES"
     gene            complement(2229..3149)
                     /locus_tag="PKH54_09705"
     CDS             complement(2229..3149)
                     /locus_tag="PKH54_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD00211.1"
                     /translation="MAGIQARNISKRWGDLAAIDDVSFDAGPGQVFGMLGPDGGGKTT
                     LFRILTTLLLPDSGTATVDDLDIRRDYRRIRSRVGYMPGRFSLYQDLTVEENLTFYAS
                     VFGTTIDENYHLIKDIYRQIEPFRDRRAGALSGGMKQKLALSCALIHKPAVLFLDEPT
                     TGVDAVSRRELWEMLLKLRDQGLCIAVSTPYMDEAAMCDRVALMMRGRFMAQDTPDGI
                     TRGFADRLLAVRGDRMPALLRDLRTMPGVLSCHAFGDSHHVTVADDLDLDGLAGGLAA
                     RGHTDVQITPIDPGIEDCFMALARDGGDTK"
     gene            complement(3118..>3847)
                     /locus_tag="PKH54_09710"
     CDS             complement(3118..>3847)
                     /locus_tag="PKH54_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOD00212.1"
                     /translation="RQIELQREQLVTRMNGLKTRVTDVYTQTAALNQQIAAAEKEKRR
                     VQELLAGGSATGKQLDDVTAQIEVLKRQRAAAAQSMKTGNRVIDDERAAMEVQLAQFD
                     DQIARCELKSPVSGTVLVKYAQQGEFTATGKALFKLADMKNMFLRAYVTADQLSRMKL
                     GQAVKVRADFGPEGSRDYDGRIAWVATKSEFTPKTIQTRDERANLVYAVKVAITNDGF
                     LKLGMYGSIPMEAENGGNPGAEHK"
BASE COUNT          695 a         1178 c         1200 g          774 t
ORIGIN      
        1 caggccgccc cgggtgccgt ttaccgtgtt cgccgcgatc atcaccgcgg ccgagcgctg
       61 gcggaccatg tcgcgttcaa agccggacgg gatttccagg atgatgtcgg cggtgtcgtc
      121 ctcgatgccg cgcagcgccc gttcatagct gtcggccacc tgggtgacgc ggaagtatcc
      181 ggaagccgag gccttctggg tcaggcggcg cgacatgggg ctgtggtcgt ggtcaacgat
      241 ggacaggttc atgtcccgca cgtcgaagtt ggcgaccagc ggaaaaacaa gcaggcttac
      301 gaacgggaac atgaagacca tgcgcggcag gaacttgttc cggccgaact gcttgaattc
      361 cttttccagc aggtatcgaa gcatggtcac tccagccgcg tgcggtgccg tttcaggctt
      421 gccgcgataa ggaacaccgc catcccggac aggacggcca gttccttcct gaccgactcg
      481 aaatcaaggc cgcgaatcat gatcttacgg accgccgcga tgaaccactt ggcgggaacc
      541 gcctgggaaa gccactgcag cggccacggc atcgtttcga tcgggaatat catccccgac
      601 agcatgatga ccggcagcat catcgccatg ccggaaatca gcagggcggt gctctgggtg
      661 ttggccaccg acgaaatcag gatgccaagc gccagcgaca tgaagatgta aatcagcgcc
      721 gttccgatca gcagggccag gctgccgacg atcggcacgc ccaggacgta caccgccatc
      781 aacaggatgg tcgccaggtc aatcaccgat atgacgaagt atggcaccgt cttggccagg
      841 atgatttgca gcgggcggac cggggacacc agcaggacct ccatcgtccc ggtttccttt
      901 tcgctggcga tcgaaatcga cgtcatcatc gcgcagatca gcagcaggat catgcccatg
      961 acgccgggga cgaagttgta cgcgcccttc atcgacgggt tgtacagcag cttcacctcc
     1021 gggattatca ggtacggcac ggacgcgtcg cgcagcagtt cctgctggta tccggcaatg
     1081 atgttctggg catagtttac aagggttttc gcggtgttcg ggtcggtacc gtcggctatc
     1141 agttgaacct gggccgcgcc ggtgtgcagc atttcttcct ggaagttttc ggaaaagaca
     1201 accgccagcc cgacctcgcc gcgcgcgaat acgtcctgta tctgcctggg gtcggaaagc
     1261 gtccgcgaca ggatgaagta gtcgttggcg gcaagccgat cgacgatggc gcgggtcgcg
     1321 tcgtcggggg caggggcgta cacggcaagc ctggtgttct tgacctcggt cgtaatcgca
     1381 aacccgaaca aaaaaagcat caggatcggc aggccaagca gtattgcggt tgtaagccgg
     1441 tccctgaaga tatggcggaa ctccttggcg acaaaggcga aaaactgctt catgtcagct
     1501 ttccccccgt tcggccctgc gtgccaggct gaagaagact tcgttcatcg tgcgggcttc
     1561 gtgctgccgc ttaagctcgc gggggctgtc cagcgcctgg atgcggccat cgacaaggat
     1621 gctgacgcga tcgcagtatt cggcctcgtc catgtagtgc gtcgtgacga aaatcgtgat
     1681 accgctttcg gccgcctggt aaatcagttc ccagaactgg cggcgcgcgg ccggatcgac
     1741 accgcctgtc ggttcatcca ggaagacgat gcgcgggttg tggaccatgg cgaccgagaa
     1801 cgccagcttc tgcttccacc ccagcggaag gcttccgacc atcgcgtttc gcccgtccat
     1861 gaagttcagg agcgtcagga tttcgtccgt cctttcgggg atacggcgtg ccggaacgcc
     1921 gtagatcccg gcatacaggc ggatgttttc ccagaccttc aggtcatcgt aaagggcgaa
     1981 cctctggctc atgtaaccga tggaagcctt taccatttcc ggctggcgcg acacgtcgaa
     2041 gccggcgacc gtgccgacgc ccgatgtcgg cctggacagg ccgcacagga tgcgcatcgc
     2101 ggtcgtcttg cccgccccgt tcgcgcccag gaagccgaat atctcgcctt gctccacctc
     2161 gaagctgatt gcatcgacgg ccgtgaagtt cccgaaacgt ttcgtaagtt cgcgtgcggt
     2221 gatgaccttc atttcgtgtc acccccgtcc cgcgccagcg ccatgaagca gtcttcgata
     2281 ccggggtcaa tcggggtgat ctgcacgtcc gtgtgtccgc gggcggcaag gccaccggca
     2341 agcccgtcga gatccaggtc atcggccacc gtcacgtggt ggctgtcgcc gaacgcgtgg
     2401 cacgacagga cccccggcat cgtgcgcagg tcgcgcagaa gcgccggcat gcggtcgccg
     2461 cgtacggcca gcagtcggtc cgcaaagccc cgggttatcc cgtcgggcgt gtcctgcgcc
     2521 atgaagcgtc cccgcatcat cagggcgacg cggtcgcaca tggccgcctc gtccatgtaa
     2581 ggcgtggaaa ccgcgatgca gagtccctgg tcgcgcagct tcagcagcat ttcccacagt
     2641 tcgcggcgcg aaaccgcatc gacgccggtt gtcggttcat ccaggaagag cacggccggt
     2701 ttgtggataa gcgcgcatga aagggccagc ttctgcttca tgcccccgga aagcgcgcct
     2761 gcgcggcgat ccctgaatgg ctcgatctgc cggtatatgt ccttgataag gtgatagttt
     2821 tcgtcaatcg tcgtgccgaa gacggatgcg taaaatgtca ggttttcttc aaccgtcagg
     2881 tcctggtaca ggctgaagcg gccgggcatg tacccgacgc ggctgcggat ccggcggtag
     2941 tcgcggcgta tgtccaggtc atcgaccgtg gctgtaccgg agtccggcaa cagcagcgtt
     3001 gtcaggatac ggaacagcgt cgtcttgcca ccgccgtcgg ggccgagcat gccgaagacc
     3061 tggccggggc cggcatcgaa gctgacgtcg tcgatggcgg cgaggtcgcc ccagcgctta
     3121 cttatgttcc gcgcctggat tcccgccatt ttccgcttcc atcgggattg acccgtacat
     3181 gccaagtttc aggaagccgt cgttggttat tgccaccttg accgcataga ccaggttcgc
     3241 tcgttcgtcc ctggtctgga tcgtctttgg cgtgaattcc gatttggtcg cgacccaggc
     3301 gatgcggccg tcgtagtccc ggctgccttc cggaccgaag tcggcccgga ccttgaccgc
     3361 ctggccaagc ttcatccggc tgagttggtc tgccgtcaca taggcacgca ggaacatgtt
     3421 cttcatgtcg gccagcttga acagggcctt gcccgtcgcg gtgaattcgc cctgctgggc
     3481 gtatttgacc aggacggttc ccgaaaccgg cgacttcagt tcgcacctgg cgatctggtc
     3541 atcgaactgg gcaagctgga cttccatcgc ggcgcgttcg tcgtcgatga cgcggtttcc
     3601 cgtcttcatg ctttgagccg cggcggcccg ctggcgtttc aggacttcga tctgggccgt
     3661 gacgtcgtca agctgctttc cggtcgcgct tccgccggca agcagttcct ggactcgtcg
     3721 cttttccttt tcggcggcgg cgatctgctg gttcagtgcg gcagtctgcg tatagacatc
     3781 ggtgacacgc gtcttcaggc cgttcatgcg ggttacaagc tgttcgcgct gcagttcgat
     3841 ctggcgg
//
LOCUS       DAOPPA010000313         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25792_length_3839_cov_3.916755, whole genome shotgun sequence.
ACCESSION   DAOPPA010000313 DAOPPA010000000
VERSION     DAOPPA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25792_length_3839_cov_3.916755"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1550)
                     /locus_tag="PKH54_09715"
     CDS             complement(177..1550)
                     /locus_tag="PKH54_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOD00213.1"
                     /translation="MGRLNPLIFREYDIRGLVGPDLDDDTVGTIARAFGTWLRGHGAT
                     RAVVGADCRLSSPGFREVVIAGLNDTGIDVIDLGMVTTPLTYFALHHLDIGAGVMITG
                     SHNPKEFNGLKMCAGKKSVFGAQIQEIRRIAESGQFQHGRGGRRTFDIVPDYMERIVS
                     DCRIRPERRFKVVVDAGNGTGGITAVPLLRRLGCEVIEIFCSPDGNFPNHHPDPTVME
                     NLVHLQEAVVSNGADVGFGFDGDADRIGVVAEDGSVVMGDMILLTLALDLLKELPGAE
                     IVSEVKGSQVLFEQVKLAGGRPVMWKVGHSLIKARMAETDVPLGGEISGHMFFNYRWY
                     GFDDAVYAATRYLEVLDAFSGPASAMLASIPKTFSTPELRVDCPDQIKVDVVEHLKDH
                     YQGAGHDVVTIDGARVLFQGGWGLVRASNTQPVLVLRFEADTPENLEIIRSDMMERAA
                     AAVRSFS"
     gene            1734..3485
                     /locus_tag="PKH54_09720"
     CDS             1734..3485
                     /locus_tag="PKH54_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00214.1"
                     /translation="MTFSNLRCFNPLKALRRGAFTLAVMAAVLAASCDEESGKCVDST
                     DCARSMACRQDACIVVDCSNPGDCLDDERCVPPQYGGIDHAGNTCTAACNVFFKCRDN
                     EVCNDGICIARNSPSDVMDVPDEDDDTGDMPDDGRSDATDEGIAADTADAGSHPQFPD
                     CKTCSTNAECGEGYSCLPVGPEKHCLRDCERDADCMAGYTCYQASAEKSCLPVSFACA
                     PCAFDSPCDEGKCCDFGTGQCRICQDECHGCTYDYECRDGMRCFKNTGFAQGICVPGC
                     ADVGCADTTNFECADNDRGVPVCKPLGDGCRGCTEPNPYPYDGGCVECTRDLDCDDGD
                     ICRMPNHTCVPDTCGDGHVLCESDMECHQCCSDDDCLRFPESTGVCLEDLTCEGAVPC
                     GGLCTDDFPACVVIDGLEQCVQCASDEDCKVINSDCTCNQNYYACTDTDGAICQLADR
                     ECGATCEDSSDCPPTTTGAEQQCALIDGGPTGYCYDPAGSCDGSISCCGPGRKCYDIM
                     SILMGGSGMPMPVGTTMYCDCEVAEDCISGIDCMDMSILCIAGMLMEMFCPGGQLPAA
                     MPPKMCLNFADLLGGIM"
     gene            complement(3590..>3839)
                     /locus_tag="PKH54_09725"
     CDS             complement(3590..>3839)
                     /locus_tag="PKH54_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00215.1"
                     /translation="TFLIAGGHGWDSPESSLRLLNPNERIVQDGKTTVRISFQTELPS
                     FVTGRVGLSAIEDLTGRVLIVGGFSGDDVPAEAAMYVP"
BASE COUNT          828 a         1173 c         1099 g          739 t
ORIGIN      
        1 tggaatcgac gggatccgta acgtcgccgg ccgccgggga accggcacag cccgggaaga
       61 acgcaagggg caccgcgacc aaagcaatcc ggaaacggat ggaattcaac atccttgtcc
      121 tgcaggaaaa ccgccacggg atcttatcaa atgggcctga tgcgggcaaa cggcggtcaa
      181 ctgaagctgc ggactgccgc ggcggcccgt tccatcatgt ctgaacggat gatttcaagg
      241 ttttcggggg tgtccgcctc gaaacgcaac accaggacgg gctgggtatt ggacgctcga
      301 acaagccccc atccgccttg gaacaacacg cgggcgccgt cgattgtcac aacatcgtga
      361 ccggcgccct ggtaatgatc tttcaggtgc tcgacgacat cgaccttgat ctgatccggg
      421 caatcgaccc gaagttccgg cgtgctgaag gtcttcggaa tcgatgccag catcgccgaa
      481 gccggaccgg aaaaggcgtc aaggacttcc aggtacctgg ttgccgcata caccgcatcg
      541 tcgaaaccgt accagcggta attgaagaac atgtgcccgg agatctctcc gcccagcggt
      601 acatccgttt cggccatgcg cgccttgatc aggctgtggc cgaccttcca catcaccgga
      661 cggccaccgg ccagcttcac ctgctcgaac agaacctgac tccccttgac ctcggaaacg
      721 atttccgcac cgggcagttc cttcagaaga tcaagcgcca gcgtcagaag aatcatgtcg
      781 cccatcacca cggaaccgtc ttcggccacg actccaatcc gatccgcgtc gccgtcaaag
      841 ccgaacccga cgtccgcacc gttcgaaacc accgcctcct gaagatgcac aaggttttcc
      901 atgacggtcg gatccgggtg gtggttggga aaatttccgt cgggactaca gaatatctcg
      961 atgacttcac agccaagacg acgcagcaat ggaactgccg ttattccgcc cgttccgttg
     1021 ccggcatcga ccacgacctt gaacctgcgt tccggcctga ttcgacagtc cgagacgatt
     1081 ctttccatat agtcgggaac gatgtcaaaa gtacggcgcc cgccgcgtcc atgctggaac
     1141 tgtccggact ccgcgatccg ccggatttcc tgaatctggg cgccaaagac ggacttcttg
     1201 cccgcacaca tcttcaaccc gttgaattcc tttgggttat ggcttcccgt aatcatgacg
     1261 ccggcgccga tatcgagatg gtgaagcgca aaatacgtca gcggcgtggt caccatgcca
     1321 aggtcaatca cgtcgattcc cgtgtcgttc agcccggcaa tcaccacttc acggaacccg
     1381 ggcgaagaaa gacggcagtc cgcgcccaca accgccctgg tcgctccgtg tccgcgaagc
     1441 caggttccaa acgcccgcgc gatggttcca accgtatcgt cgtcaagatc gggcccgaca
     1501 aggcccctta tgtcgtattc cctgaagatg agtggattca aacggcccat tgacagcctc
     1561 cgttcggcag acaggcgatc ttatgccacc agcgccgtta ttgtaagctc cggggccttt
     1621 catggcggcc gaacgaaaag gacgcagact tgccgtcctt atccgacgac tggattgacg
     1681 actgaattga taatagaatc gtcagtcgat aattcgaacg acaggtgaca aagatgacat
     1741 tttccaacct gcgttgtttc aatccgctta aggccttgcg gcgcggcgca ttcacgcttg
     1801 cggtgatggc cgccgtgctg gcagcttcct gcgacgagga atccggcaaa tgcgtggatt
     1861 cgaccgactg cgccaggtcg atggcatgcc gtcaggacgc ctgcatcgtc gtcgattgct
     1921 cgaatcccgg ggactgcctt gacgatgaaa gatgcgtgcc gccgcagtac ggtggaatcg
     1981 accacgccgg caacacctgc acagcggcgt gcaacgtatt tttcaagtgc cgggacaacg
     2041 aagtttgcaa cgatggcatc tgcatcgctc ggaactcgcc ttccgatgtg atggatgtcc
     2101 cggatgaaga cgacgacacg ggcgacatgc cggatgacgg ccggagtgat gcaaccgatg
     2161 aaggcattgc tgccgatacg gccgatgccg gatcccatcc acaattcccg gactgcaaga
     2221 catgctcgac caacgccgaa tgcggtgaag gatactcctg ccttccggtg ggccccgaga
     2281 agcactgcct gcgggactgc gaacgggatg cggactgcat ggccggatac acctgctatc
     2341 aggcgtccgc cgaaaaaagc tgtctgccgg tttcgttcgc ctgcgctccg tgcgccttcg
     2401 attcgccgtg cgatgaaggc aaatgctgcg acttcggcac cggccagtgc agaatttgcc
     2461 aggacgagtg ccacggatgc acctatgact acgaatgccg ggatggcatg cgctgtttca
     2521 agaacactgg attcgcccag ggcatctgcg tgcccggatg cgcggacgtg gggtgcgccg
     2581 acacgacgaa ctttgagtgc gcagacaacg accggggcgt gccggtgtgc aagccgcttg
     2641 gcgacggctg ccgtggctgc accgaaccga acccgtaccc ttacgatggc ggttgcgtcg
     2701 aatgcacacg cgacctggac tgcgacgacg gcgacatctg caggatgcca aaccacactt
     2761 gcgtgcccga tacgtgcggc gacgggcacg ttctgtgcga atcggacatg gaatgccacc
     2821 agtgctgctc ggacgacgac tgcctgcgct tcccggaatc gaccggcgtg tgtcttgaag
     2881 acctgacctg cgagggtgcg gttccatgcg gtgggctctg caccgatgat tttccggcct
     2941 gcgttgtgat tgatggtctg gaacaatgcg tccagtgcgc aagcgacgag gactgcaagg
     3001 tgatcaacag cgactgtaca tgcaaccaga attattatgc ctgcaccgac accgacggcg
     3061 ctatctgcca gcttgccgat cgggaatgcg gtgcgacctg cgaggattct tccgactgtc
     3121 caccgacaac gaccggcgcc gagcaacagt gcgcccttat cgacggggga ccgacgggat
     3181 actgctatga cccggcaggt tcttgcgacg gctcgatttc ctgctgcgga ccgggtcgca
     3241 agtgttatga catcatgagc attcttatgg gtggcagcgg gatgcccatg ccggtcggga
     3301 cgaccatgta ttgtgactgc gaagtcgccg aagactgcat cagcgggatt gactgcatgg
     3361 acatgtcaat tctgtgcatt gccggcatgt taatggaaat gttctgccct ggcggtcagc
     3421 ttcccgccgc catgccgccc aaaatgtgtc ttaactttgc cgatcttctt ggcggcatta
     3481 tgtgaccgag actccgacca agcgtcaata tccaccggcc ccgacagcat caccggcccc
     3541 gacagcatca agtttttttg aaaaatgatc ggcaggctcg gacacaaacc tatggcacat
     3601 acatagccgc ttcggcagga acgtcgtcac ccgagaagcc gcccacgata agcacccttc
     3661 cggtcagatc ctcgattgcg gaaaggccga cacgcccggt gacaaatgac ggcagttccg
     3721 tctggaacga aatccttacg gtcgtctttc catcctgaac aatccgctcg ttcggattca
     3781 gaagacgaag gctggactcc ggagaatccc acccgtgtcc gcccgcgatc aggaacgtg
//
LOCUS       DAOPPA010000314         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25801_length_3838_cov_3.762622, whole genome shotgun sequence.
ACCESSION   DAOPPA010000314 DAOPPA010000000
VERSION     DAOPPA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25801_length_3838_cov_3.762622"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKH54_09730"
     CDS             complement(<1..623)
                     /locus_tag="PKH54_09730"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD00216.1"
                     /translation="MYSLRIENDKGEIRRIDVDKDVYSIGRLKQNDLVLSDINVSRRH
                     AVLEVRAEGLVLVDPGSKYGIRMNSTQNPPEVLLHGGDTFVLGDYKIELIPSESEVLP
                     GASATIRMTSLNEKDVQEPVAAEKGDVAPSGAAVLIEDAVGVPYTVAVSEDESLRVLL
                     EMRKRSLSGAGHKTDGHEADGFSLDPSVAEERRTAVRRSLLIALMLAA"
     gene            complement(683..>1128)
                     /locus_tag="PKH54_09735"
     CDS             complement(683..>1128)
                     /locus_tag="PKH54_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD00217.1"
                     /translation="DPGTRRDAVAALGHFGDRRAVGIAARLLSDPAVEVVRAAAGAID
                     WLGVGGVESSVAAAVMNPATPDDVAMMLLRSLTPSLKMNQKSPKVKKIVARLLSCGRR
                     LEFDNGVFKVTLDVGQELLAGGFQLPEVTAAPAEAGARQTGGAHN"
     assembly_gap    1129..1138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1139..2068)
                     /locus_tag="PKH54_09740"
     CDS             complement(<1139..2068)
                     /locus_tag="PKH54_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOD00218.1"
                     /translation="MRRIELVAALLVLTGCASCPGTQSRRPAPREIARMDVRQEPVCT
                     RRPIETSLDTEPLTPIRREHPRIGRLRGKGSLSIGTPVRGFVVDGMELPLRGNHHKVM
                     KEQAGRGTNHAIDDLVKAIQKAAGDVARRHSGSILPVGNLSRGGGGQIRWSISHRSGR
                     DADFGFYLVDSGGRQVFQESMVHIDRDGKASVAGVDARFDPARNWLLVKSLITNRKIS
                     VQWIFMADHLKKRLLEHARARREPAELVALASDAIAQPRGRSHDDHIHLRIYCPRDDL
                     LEGCIDIGSDRPWYSPPGPELDARYRELARLSRS"
     gene            complement(2287..>3838)
                     /locus_tag="PKH54_09745"
     CDS             complement(2287..>3838)
                     /locus_tag="PKH54_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOD00219.1"
                     /translation="AWALAPNSTREQIREAVVGGVDPLPALAGKVGSGGRLNAVSSLR
                     LLGMNVAATSPQQDEFVALGPLDYAVVFTFPFVPESVSAEAFQVNGLSAETFHVDAPD
                     TITFSFGVSPVTVEGQQEMRVVGGTIRREYDADPVQEWYGQFYYDQSRMALAGTTPAL
                     DAVLAVAPGQIELAFDEPLLTTSVQAGDLRLSEGTVLSAAMSGDSSVIFQVAGLTRDG
                     LVSYTIPAGALADAHGNPSPAFSGQFVIDDPLIERYAAVGLPQPIPDAGTISFQIEVA
                     DLITIADLDIELTITHTWVSDLDVFLIGPDGVRVELFTDVGGSGDNFTRTILDDEASA
                     PIASSQSPFTGRFRPESPLAAFDGYNAAGTWTLEITDDYAQDIGSLVAFSLVVEEDVD
                     IAPRVSSVQPIGRETDLWVPISSLSVRFSEVMAAATVTDSGNWTLVAAGDPMFAAILN
                     AFEQTGPRTESPSAGIFDSRFMSLMSSIRFSARIAWAVQGTANMASRSVDVSSGLPAA
                     ADISAAFS"
BASE COUNT          776 a         1236 c         1096 g          720 t
ORIGIN      
        1 gccgccagca tcagagcaat cagaagggat ctgcgaaccg cggttcggcg ctcctcggcg
       61 accgatggat ccagcgaaaa accgtcggct tcatggccgt cggtcttgtg accggccccg
      121 gaaaggctgc gcttgcgcat ctcgagcaaa accctcaggc tttcgtcctc ggaaacggca
      181 accgtataag gaaccccgac ggcgtcctcg attagaaccg cggccccgct cggggcaaca
      241 tcgccctttt cggccgcaac cggttcctgc acgtcctttt cgttaagcga agtcatcctg
      301 atcgtggcgc tggcaccggg aagaacttcg gactcggacg gaatcagttc gatcttgtag
      361 tcgccaagaa caaacgtgtc gccgccgtga agcagaacct ccgggggatt ctgggtgctg
      421 ttcatcctga tcccgtattt tgaaccgggg tccacaagca ccaggccttc ggctcgaact
      481 tcaaggacag cgtggcgacg ggaaacattg atgtcggaca gcacaaggtc gttctgtttc
      541 agccttccga ttgaatagac atccttgtca acatcaatcc ggcgaatctc gcccttatcg
      601 ttttcgatcc tgagtgagta catcccgtgt ccccctgacg ctccgaatgt agcacattat
      661 atcccatagg aagatatcaa ggttaattgt gtgctccccc tgtctgccgg gctccggctt
      721 cggcgggggc cgccgttacc tccgggagct ggaatccccc cgccagcagt tcctggccca
      781 cgtcaagcgt caccttgaag actccgttgt cgaactcaag ccttctgccg catgacagaa
      841 gcctggcaac aattttcttc accttcggcg acttctggtt catcttcagc gaaggcgtca
      901 aacttcgcag gagcatcatc gcgacatcat ccggcgtcgc cggattcatg acggctgccg
      961 cgacactgga ttcgaccccg ccgacgccaa gccagtcaat ggccccggcc gccgctcgaa
     1021 ccacttcaac ggccggatcc gaaagcagcc tggccgcgat gccgacggcg cgcctgtccc
     1081 cgaagtgtcc cagggccgct accgcgtccc ttcttgtccc agggtcgcnn nnnnnnnncg
     1141 accgcgacag ccttgccagt tcccgatacc tggcatccag ttcgggacct ggtggtgaat
     1201 accacggtcg gtcggaaccg atgtctatgc aaccctcgag caaatcgtca cgaggacagt
     1261 agatgcgcaa atgaatatgg tcatcgtgac tccggccgcg cggctgggcg attgcatccg
     1321 acgcgagcgc gaccagttcg gcaggttccc gcctggccct ggcatgctcc agaagccgct
     1381 tcttcaggtg atcggccatg aaaatccact gcaccgatat cttccggttg gtgattaatg
     1441 acttcaccag caaccagttc ctcgccggat cgaagcgcgc gtccactccg gcgacggacg
     1501 cctttccgtc gcggtctatg tgaaccatcg attcctggaa gacctgtcga ccgcccgaat
     1561 ccacaaggta gaacccgaag tcggcgtcac ggccggatct gtgcgagatc gaccatcgaa
     1621 tctgaccacc accgcccctg gaaagattcc cgaccggaag gattgacccg gaatggcgac
     1681 gagccacgtc gcccgccgcc ttctggatgg ccttcacaag atcatcgatt gcgtggttcg
     1741 ttccgcgtcc ggcctgttcc ttcatgacct tgtgatgatt cccgcgcagc ggaagttcca
     1801 tgccatcgac aacaaacccg cgcaccggcg tgccgatcga cagggaaccc tttccccgca
     1861 ggcgcccgat gcgcgggtgt tcccgccgga tcggggtcaa cggctccgtg tccaggcttg
     1921 tctcaatcgg gcgccttgtg cagaccggtt cctgccggac atccatcctg gcgatttcgc
     1981 ggggcgccgg tcgcctggac tgggttcccg gacaggaagc gcagccagtc aaaacaagca
     2041 gcgccgcaac cagttcgatt cttcgcatcg gaaacacctc gggcaggcca ccgcatgccg
     2101 gccatccggc actttataac catacggatg aaatcgccaa actgcagata cctgtcggaa
     2161 tggccatcca gtccgacggt gacggcttga cccacggcaa cagccctgct agataacttt
     2221 gaatcagttt ctggtcgaac tccaggtgtc tgaacagata ctcttcactc aggaagtcaa
     2281 acgcatctat gaaaacgccg ccgaaatatc ggcggcggcc ggcaagccgc tggacacatc
     2341 gacgcttctg ctggccatgt tcgcggtgcc ctgcacggcc caggcaatcc tggctgaaaa
     2401 ccggattgac gacatcaggc tcatgaacct gctgtcaaag attcccgccg atggggattc
     2461 ggttcttggc ccggtctgct caaacgcatt aagaatcgcg gcaaacatcg gatccccggc
     2521 ggccaccagc gtccagttgc cactgtcggt tacggtggcg gcagccatga cttccgagaa
     2581 gcggacggac aggctactga tcggtaccca caagtccgtc tcgcgaccga tcggttgcac
     2641 cgaactcacc cgcggggcaa tatcgacgtc ttcctctacg acgagcgaga atgcgaccag
     2701 tgagccaatg tcctgggcat agtcgtcggt gatctcaaga gtccaggtgc cggcggcgtt
     2761 atagccgtcg aaggccgcca ggggggattc gggacggaaa cggcccgtga atggcgactg
     2821 gctggatgca atcggggcac tcgcttcatc gtcaaggatg gtgcgcgtga agttgtctcc
     2881 ggacccgccc acgtccgtga acaactcgac gcggacaccg tctggcccaa tgaggaatac
     2941 atcgaggtcg ctgacccaag tgtgcgtgat tgtcaactcg atgtcgagat ctgcgatggt
     3001 gatcaggtcc gctacctcaa tctggaaact gatcgtgccg gcgtcgggaa tcggctgggg
     3061 cagcccgaca gcggcatacc gctcgatcag gggatcatct atcacgaact ggcccgagaa
     3121 ggcgggactg ggatttccgt gggcatcggc caaggcaccg gccgggattg tgtaggacac
     3181 gaggccgtcg cgggtgaggc cggcgacctg gaagatgacg gaggagtccc cggacatggc
     3241 agcgctcaac actgtcccct cgctgagccg tagatcgccc gcctgcacgc ttgtggtcag
     3301 caacggctca tcgaaggcga gttcgatctg gcccggcgcg accgcaagta cagcgtcaag
     3361 ggccggggtc gtgcccgcaa gggccatgcg cgactgatcg taatagaact gtccgtacca
     3421 ctcctgcacc ggatcggcat cgtactcgcg ccggatcgtc cccccaacca ccctcatttc
     3481 ctgctgcccc tcaacagtga ccggcgatac gccaaaggaa aaggtgatgg tgtcgggagc
     3541 atcgacatgg aacgtctcgg cggataggcc gttcacctgg aatgcctccg cactgaccga
     3601 ctcggggaca aacgggaatg tgaaaaccac cgcgtaatcc agcggcccca atgccacgaa
     3661 ctcgtcctgc tgtggactgg ttgccgccac attcatgcca aggagcctca ggctgctcac
     3721 cgcattgagc ctgccgcccg agcctacttt gccagccaga gctggcaggg gatcaacacc
     3781 cccgaccacc gcctccctga tctgctcacg agtcgagttc ggggccagtg cccatgcc
//
LOCUS       DAOPPA010000315         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25816_length_3836_cov_3.542978, whole genome shotgun sequence.
ACCESSION   DAOPPA010000315 DAOPPA010000000
VERSION     DAOPPA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25816_length_3836_cov_3.542978"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKH54_09750"
     CDS             <1..810
                     /locus_tag="PKH54_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00220.1"
                     /translation="GRFERRFMRPTNLAVMVSALAVLAAISGCAQITRYARGPQPRAA
                     SPGPDWKMLSPELGVNEFESSPAVQIRGRLAGISELVAEGRTLLFRMTDVEFGFGDKV
                     PEASFSLLLWGPGMPDLKPGAQISVVMNRRQPRELIVTDERGPVIRAFSGEVLPPPDG
                     VNPIHLAALPETGYSEVVLSDLLCNHTWVHSRIAVTTDESRLVMKPGEHPTLTGTHAG
                     YSMKWLMSGADSMFLDESSCQEDIEPSAWFVWMRVATLGKAKQDTFRKLRR"
     gene            complement(813..1914)
                     /gene="prfB"
                     /locus_tag="PKH54_09755"
     CDS             complement(join(813..1844,1846..1914))
                     /gene="prfB"
                     /locus_tag="PKH54_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139800744.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOD00221.1"
                     /translation="MFDNHRAKIADLSERTQVLGRYLDRPTREARIAEIEQLSTTAGF
                     WADQRKAQAIMREKNQIQKEIEAWEEPARMLRDAAELLEMADMEQAVEFDQEIDAALI
                     AVEHKVSALEFARMMSGENDTAPALVTIHSGAGGTESQDWVAMLLRMYLMYCEKAGFK
                     VAMMDEQPGDEAGIKSVTFSVEGDHAFGYLKAEHGVHRLVRISPFDANARRHTTFASV
                     DVIPDIEDTIEVEVKEEDLRIDTFRSGGAGGQHVNKTDSAVRMTHLPTGIVVQCQNER
                     SQMKNRSTAMKLLKARLYERERQMRDAEKDAREAAKMGIDFGSQIRSYVLHPYQLVKD
                     VRTGYETGNTSAVLDGELQPFIEAYLLKAAGV"
     gene            2057..2341
                     /locus_tag="PKH54_09760"
     CDS             2057..2341
                     /locus_tag="PKH54_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOD00222.1"
                     /translation="MEKSDVITFKVDGELKDALNRMKNRSEFIRSALMAALDGTCPLC
                     RGSGTLTPRQMTHWQRFSMAHHISECGECHGIVIECGDANDDESCIGGHP"
     gene            2338..3423
                     /locus_tag="PKH54_09765"
     CDS             2338..3423
                     /locus_tag="PKH54_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOD00223.1"
                     /translation="MKRRFFQALLVSAIVMIPAFSHALEGRTKVLSTTFPIWLLTGIV
                     TVGDAGISNDLLIAPSMGCPHDYSVTPNDLSRMAAADVIIINGLGMDDSLVQGKNRAS
                     GGKAVVIDSSSGIQGVMDYSHGEGARKTVAHHDKTAGDKTGHEGHHHEDHFGAGHHEC
                     PHYAEHHGNDGRQDDAAGHAHEAGHVHEGTNPHLFASPLMAARIARNIARELGSIRPS
                     SKDLYERNATAFEARMNRLTADFQAAVKRLKNVRIVTQHGAFDYLARDVGLQITAFVQ
                     DHPGYEPSAAEMLALTKTIRAGGAGAIFVEPQYPQKVPRALAAEAGIPVGLLDPVATG
                     PAGAGTDYYEKVMRQNITALEKTLGTR"
     gene            3410..>3836
                     /locus_tag="PKH54_09770"
     CDS             3410..>3836
                     /locus_tag="PKH54_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD00224.1"
                     /translation="MEPAECTDCGQKRGDLCRHDPKTTAICFDDVSVNFGGVQVLDSV
                     TARIPRGGCTCIVGPNGAGKTTLLMTLLGKISHSGAVRIGSTPDGRAARVGYVPQRLA
                     FDRGMPLTVMEFLAMGSQRRPLWLGISSDIRERATRLLEM"
BASE COUNT          747 a         1173 c         1139 g          777 t
ORIGIN      
        1 ggccggttcg aacggcggtt catgcgtcca acaaatctgg cggtgatggt ttccgcgctt
       61 gctgtccttg ccgccatttc cggatgcgcc caaatcacgc ggtacgcaag gggcccccag
      121 cctcgcgccg cttcgcccgg ccccgactgg aaaatgcttt cccctgagct cggggtgaat
      181 gaattcgaat cttcgcccgc ggtccagatc aggggcaggc ttgccggcat aagtgaattg
      241 gtggccgagg gccgtacgct cctgttccgc atgaccgacg tcgagttcgg tttcggcgac
      301 aaggtgccgg aagccagctt cagcctgctt ctgtggggcc cgggaatgcc ggatctcaag
      361 ccgggcgccc agatcagcgt cgtgatgaac cggcggcaac cgcgcgagtt gattgtgacg
      421 gatgaacgcg ggccggttat ccgggccttc tccggggaag tccttccgcc ccccgatggc
      481 gtgaatccga tccaccttgc ggccctgcct gaaaccggct actcggaagt cgtgctttcg
      541 gatctgctct gcaatcacac ctgggttcat tcaaggattg ccgtcacgac cgacgaaagc
      601 aggcttgtca tgaagccggg cgagcatccg acgttgaccg gcacccatgc cggctattcg
      661 atgaaatggc ttatgtccgg cgcggactcc atgtttcttg acgaatcaag ctgccaggag
      721 gacatcgaac cttctgcctg gttcgtgtgg atgcgggtgg ccacccttgg caaggcgaaa
      781 caggacacct tccggaaact gcgacgataa tctcagacgc cggccgcctt cagcaggtac
      841 gcttcgatga acggctgcag ttccccgtcc aggacggccg acgtgttgcc ggtctcgtat
      901 ccggtgcgga catccttcac gagctggtat ggatgaagga cgtacgacct gatctggctg
      961 ccgaaatcga tacccatctt cgcggcctcg cgggcatcct tttcagcgtc cctcatctgg
     1021 cgttcacgtt cgtacaggcg tgccttcaac agtttcatcg ctgttgaccg gttcttcatc
     1081 tgcgaacgct cgttctggca ctggacgaca attccggtcg gcaggtgcgt cattcggacg
     1141 gccgaatcgg tcttgtttac gtgctgcccc ccggccccgc cggaacggaa cgtgtcgatc
     1201 ctcagatcct cttccttgac ttcgacttca atcgtgtcct cgatgtcggg aatgacatcg
     1261 acgctggcga aagtggtgtg acgccttgca ttcgcatcga acggcgatat gcgaacaagc
     1321 cggtgcaccc cgtgttccgc cttcagatac ccgaaagcgt ggtcgccctc gacggaaaag
     1381 gtaacgctct tgatgccggc ctcgtcgcca ggctgctcgt ccatcattgc gaccttgaat
     1441 ccggcctttt cgcagtacat caagtacatg cgaagaagca tggcgaccca gtcctgagac
     1501 tcggttccgc cggctccgga atgaatggtg accagcgcgg gggccgtgtc gttttccccg
     1561 gacatcatcc tggcaaattc cagcgcggaa accttgtgct cgaccgcgat cagtgccgcg
     1621 tcgatttcct ggtcaaactc gacggcctgc tccatgtcgg ccatttcaag cagttcggcc
     1681 gcgtcccgga gcatccttgc cggctcttcc caggcctcga tttccttctg gatctggttc
     1741 ttttcgcgca tgatcgcctg ggccttgcgc tgatcggccc agaaacccgc ggtggtagaa
     1801 agctgttcta tctcggcgat tctggcttcc ctggtcggac ggtcaaagat acctcccgag
     1861 aacctgggtt ctctcactaa ggtcggctat cttcgccctg tggttgtcga acattgcatt
     1921 taccctgtca aatcgagccg cccggattgt ccccgatggc gcccttcaag tcaatctgcg
     1981 cccgaaccca gccacggctt gacatcggac gggctgtaat acatagtatt acaccacttc
     2041 cggaggggac tccgacatgg aaaagtccga cgtcatcacg ttcaaggtgg atggcgaact
     2101 gaaagacgcg ctgaaccgaa tgaagaaccg ttcggaattc atcaggtcgg cactgatggc
     2161 ggctctggac ggcacatgtc cgctttgccg cggttccggc acgctgacgc cacgccagat
     2221 gacccactgg cagaggtttt cgatggcgca ccacatcagc gagtgcgggg aatgccatgg
     2281 aatcgtgatc gaatgcggcg atgcgaacga cgacgaatcc tgtattggag gtcacccgtg
     2341 aaaaggcggt tttttcaggc cctgctggtg tcagcgatcg ttatgatccc ggcgttttcc
     2401 cacgcgcttg aaggaaggac caaggttctt tcgacgactt ttcccatctg gctgcttacc
     2461 ggaattgtca ccgtcggcga tgccgggatt tcgaacgacc tgctcatcgc gccttccatg
     2521 ggatgtccgc acgactactc ggtcacgccg aacgaccttt ccaggatggc cgcggccgac
     2581 gtgatcatca tcaatggact tggaatggac gattcgctgg ttcagggcaa gaaccgggct
     2641 tccggcggca aggccgtcgt tatcgattct tccagcggta tccagggcgt catggattac
     2701 tcacacggcg aaggcgcccg caaaaccgtc gcccatcatg acaagacggc cggcgacaag
     2761 actggacatg aaggacacca ccacgaggat cattttggcg ccgggcacca tgagtgcccg
     2821 cattatgccg aacaccatgg gaacgacggc aggcaggacg acgctgccgg acacgcgcat
     2881 gaagccggcc acgtccacga gggaaccaac ccccacttgt tcgcaagccc tttgatggca
     2941 gccaggattg caaggaacat cgcaagggaa cttgggtcga tccgaccttc gtcaaaggat
     3001 ctgtacgaac gaaacgcgac cgctttcgag gccaggatga acaggctgac ggccgatttc
     3061 caggcagccg tcaaaagact gaagaacgta aggattgtga cccagcacgg ggccttcgat
     3121 tatctggcca gggacgtcgg gctgcagata acggccttcg tacaggatca tcccggctac
     3181 gaaccgtcgg ccgccgagat gcttgccctg acaaaaacca tccgggccgg cggcgccggg
     3241 gcaatctttg tcgagcctca gtacccgcag aaggttccgc gggccctggc ggccgaagcc
     3301 ggcattccgg tcggactgct ggatccggtg gcaaccgggc cggccggggc cggaaccgat
     3361 tattacgaaa aggtcatgcg gcagaatatc accgcgctgg agaagacact tggaacccgc
     3421 tgaatgcacc gactgcggtc agaagcgcgg cgacctctgc cgccacgacc cgaagacgac
     3481 ggcgatctgc tttgacgatg tttcggtcaa cttcggcggc gtccaggttc ttgactccgt
     3541 gaccgcaagg atcccgcgtg gcggctgcac gtgcatagtc gggccgaacg gtgccggaaa
     3601 gaccacgctt ctgatgaccc tgcttggaaa gatcagccat tccggtgccg tcaggatcgg
     3661 cagcaccccg gacggccggg ccgccagggt cgggtacgtg ccgcagcgcc ttgcatttga
     3721 ccggggaatg ccgcttacgg tgatggaatt tcttgcaatg ggttcacagc gcaggcccct
     3781 gtggctcgga atttccagcg atatccgaga acgggcgacc cggcttctgg aaatgg
//
LOCUS       DAOPPA010000316         3830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25873_length_3830_cov_2.976689, whole genome shotgun sequence.
ACCESSION   DAOPPA010000316 DAOPPA010000000
VERSION     DAOPPA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25873_length_3830_cov_2.976689"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PKH54_09775"
     CDS             <1..1329
                     /locus_tag="PKH54_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOD00225.1"
                     /translation="RIRPEWTRNLASLGKAVESPVIGRTAGFDDDDCFSVSSRLQVGV
                     TIDPAPGFRFKVLIQDSRRWGEEKDFRSNSNGTLDIFQAYMEFLDINKTGLNIRAGRS
                     IMDLGSTIHVNSGSSGGTGQVFDGVWLNWFRKGVLTIDAFTTMVKTGIGPLLSDAVPE
                     DWESFSGIHLATDRPLKWLDIEAYGFYLDDAFANITQKLGTVGGRVLLFPTDGLKLSV
                     EAAAQFGRVTAWDADAVLQVKDHLAAVYLANASYQFLKARTQPKIGLFFLYASGDSTP
                     WNGQSSAFKPVFGSTHNFLGYMDQLRPSGVWDIGLNFWMYPVKELTLNLDYHFFQQTG
                     DGGFLQHFGTWAGNGSRNGVWTQDRGVLIPAGNSGFIGHELDLLAMWRPEEWLEVAWA
                     YSVFFPGAALDGAVIRGVVGTEGDIASWKDQATLGGRTAHRMLVWATLSY"
     gene            1373..2683
                     /locus_tag="PKH54_09780"
     CDS             1373..2683
                     /locus_tag="PKH54_09780"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOD00226.1"
                     /translation="MNLLFDGLSDTGKVRKRNEDSIRLESDVRLVIVADGMGGYGGGD
                     VASRIACEVVTDAVRAGYQAVDAFREALDEGSPFGRAVQEARLSVMKLLSDAAIEADS
                     CIQRAAADDAATGSEELVGMGSTVVMALFVADRAFVAHVGDSRAYLLRQGVPVRLTSD
                     HSLLQGLVDGGMISQADVARFPMKNVLTRALGVPGTVLPDVIELELAAGDRVLLCSDG
                     LYGLVRDDMIGRLGGTGDLGRCTSKLVAYANAAGGTDNISVVIVEITATDDPGTPAAD
                     AANVQAQIQRNIREKSIFRALSTAEWLRLYSSSPRLCFEDGETIFSAGQLGDGMYCVL
                     QGAVDIIRDGRSVHVFGSGTTLSEMSLTEEKIRLVNAVAKGETTVMRLSRIDFLALCD
                     RWPVTANRLLRQLQAQTAASLRDTRDELNIMKVFMARKLSSLGR"
     gene            complement(2705..3337)
                     /locus_tag="PKH54_09785"
     CDS             complement(2705..3337)
                     /locus_tag="PKH54_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00227.1"
                     /translation="MHGPAMITIVTTAILIVTTACTPTPKPKKADGLTGGTLVFSDDF
                     NRDSFGDGWDNKSGKWEIVDGWAHVEGDQNAGLWLKSPLPDKVRLEFDAKSMTPDGDL
                     KFEIFARAREHQTGYVAILGGWKNSISIIARLDEHGADRLESDARVDIGRTHHFTAVR
                     NDDTLRWYVDGRPILKFKDSEPIDGVYFAFNNWASSAWYDNVRIYDIAGM"
     gene            complement(3400..>3830)
                     /locus_tag="PKH54_09790"
     CDS             complement(3400..>3830)
                     /locus_tag="PKH54_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00228.1"
                     /translation="FGIFVAFTTAPTSVPDISGVRVQPGQGGNGGNCGAGGVGGKGGA
                     GALGGADGAGVDLTFCAAGGGAGGNGGAGGHGGGGGGGCGGPAYGIFMSGADPASVGA
                     WKTSVTFLGAGEGGAGGTGGTSFGADGTNGSNGIAANTNF"
BASE COUNT          695 a         1116 c         1170 g          849 t
ORIGIN      
        1 cgcatccgtc ccgagtggac caggaacctg gcgtcccttg gcaaggcagt cgaatccccc
       61 gtcatcgggc ggacggccgg ttttgatgac gacgactgtt tttccgtttc aagccgcctt
      121 caggtgggcg tgacgattga tccggcgccc gggttccgtt tcaaggttct tattcaggat
      181 tccaggcgat ggggcgaaga aaaggatttt cgttcaaaca gcaacggcac ccttgatatc
      241 ttccaggcgt acatggaatt cctggacatc aacaagaccg gattgaacat cagggccggc
      301 cggtcaatca tggatcttgg cagcacaatc catgtcaatt caggcagttc gggcggcacc
      361 ggccaggtat ttgacggcgt ctggctgaac tggttccgga agggcgtcct gacaatcgat
      421 gccttcacga cgatggtcaa gacaggtatc ggcccgctgc tttcagatgc cgttcccgag
      481 gactgggaat cgttctccgg cattcacctg gcgaccgaca ggccgctcaa gtggcttgat
      541 atcgaagcgt acggtttcta tctcgacgat gcctttgcga acatcactca gaaactgggc
      601 actgtcggtg gacgagttct tctgtttccg acggacggcc tgaagctttc ggtcgaggcc
      661 gccgcccagt tcggtcgggt tacggcgtgg gacgcggacg cggttcttca ggtcaaggat
      721 cacctggccg cggtttacct ggccaacgca tcgtaccagt tcctgaaggc ccggacgcag
      781 ccgaagattg gattgttctt tctttatgca agcggcgatt cgacgccatg gaacggccag
      841 agcagcgcct tcaagccagt ttttggttcc acccacaatt tcctcgggta catggatcag
      901 cttcgacctt cgggtgtgtg ggacatcggt ctgaatttct ggatgtaccc ggtcaaggaa
      961 ctgacgctta acctggacta tcacttcttt cagcaaaccg gcgacggcgg cttccttcag
     1021 catttcggga catgggccgg aaatggaagc aggaacggcg tctggacgca ggatcgcggc
     1081 gtgctgattc ccgccggcaa cagcggattc atcgggcatg aacttgattt gctggcgatg
     1141 tggcgccccg aggaatggct tgaggtcgct tgggcttaca gcgtattctt cccgggagcg
     1201 gcgctcgatg gtgcggtaat ccggggcgtg gttggaaccg agggcgacat tgcatcatgg
     1261 aaggaccagg ccacgctggg cggcaggact gcccacagaa tgctggtttg ggcgaccctt
     1321 tcgtactaag atttcgcgat tgttcctggg gcgcgttctg gcggaggcgg ttgtgaacct
     1381 gctgttcgat ggattgagcg acaccggtaa ggtcaggaag cgaaacgaag attccatcag
     1441 gctggagtcc gacgtcaggc ttgtcatcgt cgcggacggc atgggcggtt atggcggtgg
     1501 cgatgttgcc agccggatcg cgtgcgaggt cgtcaccgat gctgtcaggg ccggttatca
     1561 ggccgttgat gccttccgtg aagcccttga tgaaggatct ccgtttggcc gggcggttca
     1621 ggaagcgcgc ctgtccgtga tgaagcttct gtccgatgcg gccatcgagg cggactcctg
     1681 catccagcgt gctgccgccg acgacgccgc gaccggttcc gaagaacttg tcggaatggg
     1741 ctcgacggtc gtgatggctc tttttgttgc cgatcgtgct tttgtggcgc atgtcgggga
     1801 ttcgcgggcc tatcttctgc gccagggtgt tcccgttcgc ctgacgagtg atcattccct
     1861 tcttcagggg cttgttgacg gcgggatgat ttcccaggcc gacgtggcac gcttcccgat
     1921 gaagaacgtc ctgaccaggg ccctcggtgt tccgggaacg gtgctgcccg atgtcatcga
     1981 actggagctt gccgctggcg atcgtgtcct tctttgttcg gacgggctgt acggtcttgt
     2041 ccgtgacgac atgattggac gactgggcgg aaccggcgat cttggacgat gcacctcgaa
     2101 gctggtcgcg tatgcaaatg ccgcgggcgg cacggacaac atttccgtgg tgatcgttga
     2161 aattacggcg accgacgatc cgggaactcc ggccgccgat gccgccaatg tccaggccca
     2221 gattcagcgc aacatacgtg aaaagtcgat ttttcgggcg ctttcaacgg ccgaatggct
     2281 gaggctgtat tcgtcttcgc ccaggctgtg cttcgaggac ggcgaaacca tcttttccgc
     2341 gggacagctt ggcgatggaa tgtactgcgt gcttcagggg gctgtcgata tcatacgcga
     2401 cggtcgttcc gtccatgttt tcggttctgg caccaccttg agcgaaatgt ccctgaccga
     2461 ggaaaagatt cgtcttgtca acgcggtggc aaagggcgag accacggtga tgcgtctttc
     2521 acgcatcgat tttcttgcat tatgcgatcg gtggccggtt accgcgaaca ggttgcttcg
     2581 ccagcttcag gcccagacgg cggccagcct tcgcgacacc cgggacgaac ttaacatcat
     2641 gaaggtgttc atggccagga agctgagtag cctcggccgt tagcccgaat ccgcgagagt
     2701 cggttcacat gcccgcaata tcgtaaatgc gcacgttgtc gtaccaggcg ctggaagccc
     2761 agttgttgaa tgcgaaatag acgccgtcga tcggttcgga atccttgaac ttcagtatcg
     2821 ggcgcccgtc aacgtaccag cgcagggtgt cgtcgtttct gaccgcggtg aagtgatgag
     2881 tccggccgat atcgaccctg gcatccgact ccaggcgatc ggccccatgc tcgtcaaggc
     2941 gggcgatgat gctgatgctg tttttccagc cgcccagaat tgccacgtat cctgtctggt
     3001 gttccctggc cctggcgaaa atctcgaact tcaggtcgcc atccggggtc atgcttttcg
     3061 cgtcgaattc aagcctgacc ttgtcgggaa gagggctctt caaccaaagc ccggcgttct
     3121 ggtcgccttc aacgtgggcc catccatcga cgatttccca ttttccgctt ttgttgtccc
     3181 agccgtcgcc gaacgaatcc cggttgaagt cgtcggagaa gaccagcgtt ccgccggtca
     3241 gtccgtcagc ctttttcggc tttggcgtcg gggtacacgc cgttgtgaca ataagaattg
     3301 ctgtcgtgac aatcgtaatc attgcaggtc cgtgcattgc cgtccacctg ttgaagttgt
     3361 gaataattca atccagacaa ttggttacaa acggaatggt cagaaattgg tgtttgccgc
     3421 gatgccgttg gagccgtttg ttccgtccgc cccgaacgac gtgccgccgg tgccgccggc
     3481 cccgccttcg cccgcgccaa ggaatgtgac cgaggtcttc catgccccaa ccgaagccgg
     3541 atccgcgccg ctcatgaaga ttccgtatgc cgggccgcca cagccgccgc cgccgccgcc
     3601 gccgtgtcca cctgcgccgc cgttgccacc cgcgccgccg ccggccgcgc agaacgtcag
     3661 gtcaacaccc gcgccatcgg caccgcccag agcgccagcg ccgccctttc caccgacacc
     3721 gcccgcgccg cagtttccac cgttgccacc ctgtccgggc tgaaccctga cgccggaaat
     3781 gtccggtacg ctcgtcggtg ccgtcgtgaa cgcgacaaag atgccgaagg
//
LOCUS       DAOPPA010000317         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25914_length_3826_cov_3.369398, whole genome shotgun sequence.
ACCESSION   DAOPPA010000317 DAOPPA010000000
VERSION     DAOPPA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25914_length_3826_cov_3.369398"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKH54_09795"
     CDS             <1..1115
                     /locus_tag="PKH54_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00229.1"
                     /translation="LRVVAEVSGAGLTTPVVQDLAINAGPPRTATGSFADVPEATGYT
                     VKLEAFPALVGGAEDTVVIYRGSTTVDVEDGLTATANVTMNPVYGDVTFTATFPAGDP
                     DIADVSYVQVTVTGNRITHDPVFYLTVAGESATGTIDNIPVGATRTFTVQAYDAADVK
                     LFEGTGAHGVTEGGDSVTVPLANVSPAAGSINVTGSFCEPDCSAAECGDDGCGGSCGG
                     CKSGATCLSGLCNTPVNWCRYQFPNLLTATSGDTVTFYGRVYHAGITDISTGVDVSPL
                     LKAQVGLGGDGTDPVTHASWNWYDAGANSGWVGTGPDANNDEYQAAVQLNTVGSFDAA
                     YRFSVDGGHSWTYCDGGDEGSSNGYSATNAGNITVK"
     gene            complement(1254..2948)
                     /locus_tag="PKH54_09800"
     CDS             complement(1254..2948)
                     /locus_tag="PKH54_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOD00230.1"
                     /translation="MNQSDLQSLLTDLIKTWENEVIEFKAASNDFNTDKIGQYFSALG
                     NEANIRNLEHAWLVFGVDDKTHKIIGTDYRPERSRLQGLKHQINQVTSPSLTFREIHE
                     LMHGEGRVVLFEIPAAPRGMPVAWKGQYYARAGESLVPLGLDKLDEIRNQMPGEDWSA
                     VIVPEATPADLDDQAVDTARRFFALKYSNRFTPAEIHDWPMEVFLERARLTRDRRITR
                     ATLLLLGKPESAQLLSPHPAQMTWKLVGPEKAYSHFGTPFLLNTTELYRKIRNIQIRM
                     LPDGTLLPIEIAKYDQKIVLEALHNCIAHQDYTRNARIVVTEEPDRLIFENDGAFFDG
                     QPEDYIDGHKTPRRYRNTFLVNAMAELNMIDTMGYGIHAMFVGQKNRFFPMPDYDLSE
                     SQAVRMTIYGSILDPAYSRLLIEKTDLPLQDILALDRIQKHLPINDTASTRLRRAGLI
                     EGRKPNLHISAFIANAVDKRADYIRMRSQDDEFLAKLVTDFIKTFGSASRADIDRLLT
                     QKMGDLLTDQQKSKKISNLLFKASKLGTIQNVASRRSPRWVLQNRTTKSNKSDNEK"
     gene            complement(3075..>3826)
                     /locus_tag="PKH54_09805"
     CDS             complement(3075..>3826)
                     /locus_tag="PKH54_09805"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOD00231.1"
                     /translation="EQQEDKLVEIGTVDNMGPSEDIRSVRFSGDKGYIVTFKKTDPLY
                     VLDLSDPTDPKVTGELKIPGFSTYMHFMDETHLLTIGYDADDHGDFAYFDGIALSVFD
                     VTDGSNPTMLYREIIGTRGTTSEAATNHLAFTWFKSRNALALPMLVCEGGDDGQYGHT
                     MTFNGLMVYRVTLDEGFSYLGGIPHEVPEQQEYDNYYSGGCSNWWTQSNSTVVRSIFM
                     DDWVFSVAMDEIRASALSDLANPVAVLPLKP"
BASE COUNT          769 a         1080 c         1099 g          878 t
ORIGIN      
        1 tcctgcgcgt cgtggccgaa gtcagcggag ccggtttgac gacgcccgtg gtccaggatc
       61 tggccattaa cgcgggaccg ccccggaccg ccaccggcag cttcgccgac gtgcccgagg
      121 ccaccggcta caccgtgaag ctggaagcat tccccgccct ggttggcgga gcggaagaca
      181 ccgtcgtcat ctatcgcggc agcaccacgg ttgacgtgga agacggcctg accgccaccg
      241 cgaacgtcac gatgaacccg gtttacggcg acgtcacctt caccgccacg ttcccggcgg
      301 gcgaccccga catcgccgac gtcagctatg tccaggtcac ggtcaccggt aaccgcatca
      361 cgcacgaccc ggtcttttac ctgacggtcg cgggcgaatc cgccaccggc acgattgaca
      421 acatcccggt gggcgccacg cgcacattca ccgtccaggc ttacgacgcc gccgatgtga
      481 aacttttcga aggcaccggc gcccatggcg tgaccgaggg gggcgacagc gtcaccgtcc
      541 ctttagctaa tgtctcgccg gcggctggat ccatcaacgt caccggatcg ttctgcgagc
      601 cggactgctc ggccgccgag tgcggcgacg acggctgcgg cggctcgtgc ggcggctgca
      661 agtccggcgc cacctgtctg tccggacttt gcaacacgcc ggtaaactgg tgccgctatc
      721 agttcccgaa cctgctgacc gccaccagcg gcgacacggt taccttctat ggtcgggttt
      781 atcacgccgg catcaccgac ataagcaccg gcgtcgatgt cagcccgctg ctgaaggccc
      841 aggtgggtct gggcggtgat ggcacggacc cggtcaccca tgcatcatgg aactggtacg
      901 acgccggcgc caacagcggt tgggtcggca ccggcccgga cgccaacaac gacgaatatc
      961 aggccgcggt gcaactgaac accgtgggca gcttcgacgc ggcgtaccgc ttcagcgtcg
     1021 atgggggcca cagttggaca tactgcgacg gcggcgacga gggctcgtcg aacggctact
     1081 cggccaccaa cgctggcaac atcaccgtta aatgatctgt cttctgattc ctgctgggcc
     1141 ttgcccttgt gcgggacgcg aaccaaatca gccgttaaaa ctttaattga tgtctgattg
     1201 gacaggtttc aaggataaaa agtcatttag acacaggtag ttaacgcgga ttattacttt
     1261 tcgttatcac ttttattact tttggttgtg cgattttgga gcacccagcg tggggatcgc
     1321 ctggaagcga cattctggat tgtgcccagt ttagatgcct tgaatagcaa gttgctgatc
     1381 tttttggatt tttgttgatc cgtaagtaag tcgcccattt tctgggtcaa caaccggtca
     1441 atgtcagccc ggctggcaga gccgaatgtt ttgatgaagt ccgtcacgag tttggccaga
     1501 aattcatcat cctgacttct catccggatg taatctgccc gtttgtccac ggcattcgca
     1561 ataaatgcag aaatgtgcag attcggcttc ctgccttcaa tcaatccagc tctacgtaac
     1621 cttgtcgatg ccgtgtcatt gatcggaagg tgtttttgga ttcggtccag ggccagaatg
     1681 tcttgaagcg gcaaatcggt cttttcaatc agcaggcgac tgtatgccgg gtccaggata
     1741 gaaccataaa tggtcattcg cacagcctgc gattcggaaa gatcatagtc cggcatcggg
     1801 aaaaatcgat tcttctgacc cacaaacatg gcgtggatgc catagcccat ggtgtcgatc
     1861 atgttcaatt cggccattgc attcacaagg aatgtgttgc ggtatcgccg cggtgttttg
     1921 tgaccatcga tatagtcctc tggttggccg tcaaagaacg ctccgtcgtt ttcaaaaatc
     1981 agccggtcag gttcttcagt cacgacgatt cgggcgttgc gcgtgtaatc ctgatgggca
     2041 atgcagttgt gaagggcctc aagcacgatt ttctggtcat atttggcaat ttcgatcggc
     2101 aacagggttc cgtccggcag catccgaatc tggatgttgc ggatttttcg atacagttcg
     2161 gtggtattca gtaagaacgg tgtcccgaaa tgactgtatg ccttttccgg tccgaccagc
     2221 ttccaagtca tctgggctgg gtggggcgaa agcaattgcg ccgattccgg cttccccagc
     2281 agaagtagtg tggctctggt aatccggcgg tcccgggtta accgggcacg ttcaagaaat
     2341 acttccattg gccagtcgtg aatttcagca ggtgtgaacc tgttggaata cttgagagcg
     2401 aagaaccttc tggccgtatc aaccgcttgg tcgtccaggt ctgccggtgt tgcctcggga
     2461 acgatcacag ccgaccagtc ctcgccaggc atctggtttc gaatctcgtc cagtttgtcg
     2521 agtccgagag gaaccagact ctcgcctgcc cgtgcatagt actgtccttt ccaggcaacc
     2581 ggcattccgc gtggggcggc aggtatctcg aacaggacga cgcggccttc accgtgcata
     2641 agttcatgaa tttcgcgaaa tgtcagcgac ggggaagtga cctggttgat ctggtgtttc
     2701 aggccctgaa ggcggcttcg ttcgggccgg tagtcggttc cgatgatctt gtgggttttg
     2761 tcgtcaacgc cgaacaccaa ccaggcatgt tcaagattcc tgatatttgc ttcgtttccc
     2821 agcgcagaaa agtattggcc gatcttatcg gtattgaaat cgttgctggc agccttgaac
     2881 tcgatcactt cattttccca ggtcttgatt aagtcggtca gaagtgactg aagatcagat
     2941 tgattcattg ttgcccccga aaaggccagc ttcggcgcat cttgtgacct tcggattgta
     3001 gcttaaagaa gagattccag aagactgaac tgaggccaaa aaccgggact acttgaatgt
     3061 ggaccggatt acccttaggg cttcagaggc aggactgcga ccgggttggc caggtcggac
     3121 agggcagatg cgcggatttc gtccatggcg acactgaaga cccagtcgtc catgaagatg
     3181 ctgcggacaa ccgtcgagtt cgactgggtc caccagttcg agcagccgcc gctgtaatag
     3241 ttgtcgtact cctgctgttc cgggacctcg tgcgggatgc cgcccaggta actgaagcct
     3301 tcatccagcg tcacccgata gaccatcagt ccgttgaacg tcatcgtgtg accgtactgg
     3361 ccgtcgtcgc cgccttcgca gaccagcatc ggcagggcca gggcgttgcg cgacttgaac
     3421 caggtgaacg ccaggtgatt cgtggccgct tccgacgtgg tgccgcgcgt gccgatgatt
     3481 tcgcggtaca gcatggtcgg attgcttccg tccgtcacgt cgaacaccga cagggcgatc
     3541 ccgtcgaagt acgcgaagtc gccgtgatcg tccgcgtcgt acccgatggt cagcaggtga
     3601 gtttcgtcca tgaagtgcat gtacgtcgaa aatcccggga ttttcaattc gccggtgacc
     3661 ttcgggtcgg tcgggtctga caggtccagc acgtaaagcg ggtcggtctt tttgaacgtg
     3721 acgatgtacc ccttgtcgcc gctgaaccgg accgaccgga tgtcttcgga cggtcccatg
     3781 ttatcgacgg ttccgatttc gaccagcttg tcttcctgct gctcaa
//
LOCUS       DAOPPA010000318         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_25992_length_3817_cov_3.049176, whole genome shotgun sequence.
ACCESSION   DAOPPA010000318 DAOPPA010000000
VERSION     DAOPPA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25992_length_3817_cov_3.049176"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKH54_09810"
     CDS             complement(<1..475)
                     /locus_tag="PKH54_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00232.1"
                     /translation="MPAFAQETNLDSNLDKIWGKERELNVIEKKQFLPDYRHEVGIFL
                     GVIPNDPFHVFVPIGLRYEYHFMESLSFEAAGCYAPSASTQLLNDIVRDTGSAIKRGE
                     LLERMNWYVDFNIKWAPFHGKFQVMNKRITSFDAGFLVGVGVIGSKVYKQGVLETR"
     gene            complement(628..3120)
                     /locus_tag="PKH54_09815"
     CDS             complement(628..3120)
                     /locus_tag="PKH54_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00233.1"
                     /translation="MMRFEILRRAGGYLFPASAVLLVLAAIGGCQYDFTYYRSTYVAS
                     NVEFVSAVPVELTGGTPQRHCVGDDGAGVNALELAFNILSPGSQTTAGEADDRDLSIR
                     PGELIKTPDEEYLTLGVDGVDSIPCTDPDKQGQTCAPSGYRLLRPSHFSVDIDCLSPY
                     PLAGFEKACSTKQTIKDARPDTLNYQSNFTSGPFRGKPEESNLSVAVMIDQSGSMIGY
                     VEPLTQVEYSPGLKTANGAPANVSSEGFADRATDYNAFRRTAAQRLFGLLNKGDRAGI
                     FKFGEDVSADGARILCTSANDDESLNRADCFGINRDILNSPVFSDLQNTPRGRTPLWM
                     AVKDVYDFMKTETTPVKHIVVITDGPDTCAQDSPDFLPYVRYFRVSTKEWELGDQSAA
                     CSDTSYKAFLAHIHDDVYDQNGNPKPIDQIPVHITFIQMQSKGYPMIDPAQQEIACLT
                     GGHYTWINSNDLPASREENKDNLAAWAGQHPLEQALLTPITRFRNAMSGVWRMAVKVN
                     DLSAAFSAINVSFTQDFQPVVNRAEGDVIEDVTVTDTNEPDVDEPDTAVDTNTPDVLD
                     DASADVPAEDTNVVDVPSADEGPADVAEEDTNEPPVVSPGTCQEKDSPCDCTKKGTGV
                     VTGASVAVDGRIRALLVEDLTDSVFPSSASSAFVVDKKIDMRGAVRLPEGKNASDCLS
                     WFPLCSIADDLVSTVKDLNNSNVQMQARDVECLAVAFDDEFAVCSYGEMAFSSPCTGG
                     SCCWGKCIPPNDNECREDVLDEGCNYQAKAFETECSAEGKCCKGICVPKNDKECKTSE
                     LNQSSCTYKNKDDGTDCTGGKCQGGECIPSAG"
     gene            complement(3340..>3817)
                     /locus_tag="PKH54_09820"
     CDS             complement(3340..>3817)
                     /locus_tag="PKH54_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00234.1"
                     /translation="ASGQWIVSGSRTGFLHPWMTDESFACVRRPGADPIFTGRAITSM
                     PEAASVVVESCPVIRGGDMYIDWDAARFRNPMFEFNIVPGCRIDSNYKPVVIPPVRDT
                     IISFAMETGRLPFQNDIGGLPTGVIVAGDTLFTLDSSNGRMMVIDADDMTVSGSWF"
BASE COUNT          820 a         1131 c         1083 g          783 t
ORIGIN      
        1 agcgggtctc gagcacaccc tgcttgtaaa ccttcgatcc gatcacgccg acgccgacca
       61 ggaagccggc atcgaacgac gtgatgcgct tgttcatgac ctggaacttg ccatggaacg
      121 gcgcccactt gatgttgaaa tcgacgtacc agttcatgcg ctcaagcagt tcaccacgct
      181 tgatcgccga accggtgtca cggacgatgt cattaagaag ctgggtgctg gcagagggcg
      241 cgtagcagcc ggcagcctcg aaggacaggg actccatgaa gtgatactcg taacgaagtc
      301 cgatgggaac aaacacatgg aaaggatcgt tgggaatgac cccaaggaaa attcccactt
      361 cgtgcctgta gtccggcagg aactgcttct tctcgatgac gttcagttcc ctttcctttc
      421 cccagatctt gtccagattc gagtccaggt tggtttcctg cgcaaatgca ggcatccccg
      481 caaccagaac tacgaagctg atgatggtcc gaattgccca ccgtccgttc atgacaagcc
      541 ctcgcttatt gctgttggtt atccggcact tcatgccgcc aattccccaa aaaagtgagg
      601 ggcgggaggc cccccgaaaa tccaaaacta gcccgcactg ggaatgcact cgccgccctg
      661 acatttgccg ccggtgcaat ccgtgccgtc gtccttgttc ttatacgtgc aacttgactg
      721 attcaactcg ctggtcttgc attccttgtc gttcttcgga acgcaaatcc ccttgcagca
      781 cttgccttcg gccgagcatt cggtctcgaa cgccttggcc tggtagttgc agccctcgtc
      841 cagaacatct tccctgcact cgttatcgtt cgggggaatg cacttgcccc agcagcagct
      901 tccgccggtg catggggacg agaacgccat ctcgccgtag ctgcagacag cgaattcgtc
      961 gtcgaaggcc acagcaagac actcgacatc cctggcctgc atctggacat tggaattgtt
     1021 cagatcctta accgtggaaa ccaggtcatc ggcaattgag caaagcggga accacgacag
     1081 acagtcgctg gcattcttcc cttcgggcag cctgacagcg ccacgcatgt cgatcttctt
     1141 atcgacgacg aaggcgctgc tggccgacga cgggaaaacc gagtctgtca ggtcctcgac
     1201 gagcaatgcc ctgatccggc catcaaccgc gaccgaagcg ccggtgacaa cgccggtacc
     1261 cttcttcgtg cagtcgcagg gcgaatcctt ctcctggcaa gtgccggggc tgaccaccgg
     1321 aggttcgttc gtgtcttctt cagccacatc cgccgggcct tcgtccgcgg aaggaacgtc
     1381 cacaacgttg gtgtcttcgg ccggaacgtc ggctgaagca tcatccaata cgtccggggt
     1441 gtttgtatcc accgcggtgt ccggctcgtc aacgtccggc tcattggtat cggtgacggt
     1501 cacgtcctcg atcacatcac cttcggcgcg attgacaacc ggctggaaat cctgcgtgaa
     1561 actcacattg atcgccgaga aggccgccga caggtcgtta accttgaccg ccatcctcca
     1621 cacgccggac atggcattgc ggaacctggt aatcggcgtc agcagtgcct gctcaagcgg
     1681 atgctggccg gcccatgccg ccaggttgtc cttgttctcc tcacggctgg ccggaagatc
     1741 gttcgaattg atccaagtgt aatgaccgcc ggtcagacag gcgatttcct gctgagccgg
     1801 atcgatcatc ggataaccct tcgactgcat ctgtataaag gtaatgtgaa ccggaatctg
     1861 gtcaatcggc ttcggattgc cattctggtc gtaaacgtcg tcatggatgt gagccaggaa
     1921 cgccttgtag gacgtatcgg aacatgccgc gctctggtcg ccgagttccc actccttggt
     1981 gctgacgcgg aaatatcgca cgtacggcag gaagtccggc gaatcctggg cgcaggtgtc
     2041 cggaccgtcg gttatcacga cgatgtgctt gaccggcgtc gtttcggtct tcatgaagtc
     2101 atagacgtcc ttgaccgcca tccacagcgg cgtacgaccc ctgggggtat tctgcagatc
     2161 ggaaaacacc ggtgaattga gaatatcccg attgattccg aagcagtcgg cacgattcaa
     2221 gctctcgtcg tcgttcgcgg acgtgcacag aatcctggcg ccgtcggcgc tcacgtcctc
     2281 gccgaacttg aagataccgg cgcgatcgcc cttgttgaga aggccgaaca gccgctgggc
     2341 ggccgtgcgc cggaaggcgt tgtaatcggt tgccctgtcg gcgaaacctt ccgaggaaac
     2401 gttcgccggg gcgccgtttg ccgtcttcag gccggggctg tactcaacct gggtcaacgg
     2461 ctcaacatag ccaatcatcg aaccggactg atcgatcatt actgcaacgg aaagattcga
     2521 ctcttcgggc ttgccgcgga acgggccgct ggtgaaattg ctctggtaat tcagagtgtc
     2581 cggccgagca tccttgatgg tctgctttgt cgaacaagcc ttctcgaagc ccgccagcgg
     2641 atacggggac agacagtcaa tatccacgct gaagtggctt ggccgcagca ggcgatagcc
     2701 gctcggggca caggtctggc cctgcttgtc gggatccgtg caaggaatgc tgtcaacgcc
     2761 gtcaacgcca agggtcaggt actcctcgtc cggcgtcttg ataagctcgc ccgggcggat
     2821 cgacagatcg cgatcatcag cctcgccggc cgtggtctgg cttccggggc tgaggatgtt
     2881 gaaagccagt tcaagggcgt tcacccccgc tccgtcatcg ccgacgcagt gacgctgggg
     2941 cgttccgccg gtcagttcaa cagggacggc cgaaacgaac tcgacgttcg acgcaacata
     3001 ggtggaacgg tagtaggtga aatcgtactg gcatccaccg attgccgcca gcaccagcaa
     3061 aacagccgat gccgggaaaa ggtacccgcc tgcgcgccgc agaatttcga aacgcatcat
     3121 gtcaagctcc ccacttcaag gcctgatcgg acgaattaaa cgccgaaatg cacggcgggt
     3181 agtttaccct ttgtaagcca ttggttgtca acaaataatt gtcagggatg ccgggggatt
     3241 cggggccggc gggcgtcccc ctggaaagaa caacgattaa gcatcaaaat acgggatgtt
     3301 atgaaatcga aaccggtggg gaaaactgtt caaaaacggt cagaaccaac tgcccgaaac
     3361 ggtcatgtca tcggcatcga taaccatcat tcgaccgttc gatgaatcca gggtgaaaag
     3421 tgtatcgccc gccacgatga cgccggtcgg caggccgccg atgtcgttct ggaacggcag
     3481 gcgaccagtt tccatggcaa aggaaatgat cgtgtctcga acgggcggaa taacgaccgg
     3541 cttataattc gaatcgattc gacagcccgg gacgatattg aattcgaaca tcggattcct
     3601 gaacctggcg gcatcccagt cgatgtacat gtccccgccg cgaatgacgg gacagctttc
     3661 gaccacgacg cttgccgctt caggcatcga cgtgattgcc cgtccggtga atatcggatc
     3721 ggcgccaggg cgacggacgc atgcgaagga ctcgtcggtc atccagggat gaaggaagcc
     3781 ggttcgggat ccactgacga tccactgacc cgacgcc
//
LOCUS       DAOPPA010000319         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26140_length_3803_cov_3.447972, whole genome shotgun sequence.
ACCESSION   DAOPPA010000319 DAOPPA010000000
VERSION     DAOPPA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26140_length_3803_cov_3.447972"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKH54_09825"
     CDS             complement(<1..369)
                     /locus_tag="PKH54_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOD00235.1"
                     /translation="MKISRVLLATKTTLMDVGRSMHATQPLKRLLDAGDPLVADEEPG
                     HRKTVVKRTLEQAGIEVTHARRRNNVSKKFDLVIAVGGDGTVLDIARFIRNTPLLAVN
                     SSPSTSFGHFCATTSEGFPSL"
     gene            complement(372..2804)
                     /locus_tag="PKH54_09830"
     CDS             complement(372..2804)
                     /locus_tag="PKH54_09830"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00236.1"
                     /translation="MAAKKPSAEKDCRLGWKQVHRHINPSIFKFRSTRHVEKLVGVVE
                     QARAQKALELGLSIQKRNFNIYVSGASGTGKSSLLKRLVDEASRQMPTPPDWCMVNNF
                     KHPETPRVFSLPAGKGLQFRGQMDEFISSLKKNLPRAFHTQKHQETLQEILNEGLAIE
                     NEAFERLDEVAGRNNFQVKQTKEGLVTIPVVDGEAISNKEYAKLSDAQKKTIDENRRA
                     IEPAIAAFFEKNKEAEQIVHAKVQVANNRLCAETMDESMKALLAEYSSCPEVIAHLEA
                     VRASILENIPRFLAEDSDDGMTKQLIEAAMTEYKVNLLVDNSEVRGAPVVFENTPTYH
                     NLVGRIEKRVENGIYSTDFTMIKAGSLLRANGGFLILHTRELVSYPFAWEALKTVLRQ
                     RLLEIAEMGEAYQFLPTTTIRPNPIPMSCKVILVGSNFLYQLLHEADEEFGKAFQIKA
                     EFDSEVAVSDDMLQEYARFIATTSERDGLLPVSSAGVADVIEYSMRMADASDRLTLQF
                     NLVLNLLIEADHLARQEGSAIIDARHIIAARRQRKDRVSLSWDKGVEMVLNGTVIIDS
                     EGAIPGVINGLAVYQMADQTFGMPLRITAKSFRGSAGIINVEREARLSGNTFNKAVLI
                     ISGLLGDMFAKNRVPGITVSLTVEQSYGRIDGDSASCAEFCAVLSSLSRIPLRQDVAV
                     TGSLNQTGDVQAVGGVNEKIEGFFKICSERGLTGRQGVIIPAANQRNLVLEPEVEEAV
                     RKRRFNIWAARRIEEVVEIMTGLPAGTRQEDGTWEKGSVFAATDAAVKAMSEKEKPER
                     DRPGKAGKRKTT"
     gene            complement(2887..>3803)
                     /locus_tag="PKH54_09835"
     CDS             complement(2887..>3803)
                     /locus_tag="PKH54_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD00237.1"
                     /translation="SERLTKAIIFCNTRKKTEETAALVLRTGPWPAERVMVHHASLAR
                     REREDVEKAFRYFEAGILVSTSTMELGVDIGDVDAVVLFGAPETVASLQQRVGRGCRR
                     RDGMRAIFVPDGPGDEGKFAMLMNDVVEQAFPQSERLPDLSVAVQQIFSLLFENRKGL
                     RRATIRRILDPVAPGPVIDAIVDHLRTEGFLDESAGEVLRASSRLMDMGERGRIHSNI
                     ADSASIKVVDAGTGRTVGMVSARTAAGADVIIGGRNWTVTDASRGGVAVKATAGSGLE
                     SGASFGSNSGAGAFSRYLPPELRQGSAR"
BASE COUNT          670 a         1149 c         1117 g          867 t
ORIGIN      
        1 cagcgacgga aatccttcgg aggtcgtggc gcagaaatgg ccgaaacttg tcgatgggga
       61 cgagttgacc gccagcagcg gggtgtttcg gatgaacctc gcgatgtcca ggacggttcc
      121 gtcgccgccg accgcaatca cgaggtcgaa tttcttcgac acgttgtttc ttctgcgggc
      181 gtgcgtgact tcgatcccgg cctgctcgag tgtccgcttc acaacagtct tgcggtgtcc
      241 cggttcctcg tccgccacca gcggatcccc ggcatcaagc agccgcttca gcggctgggt
      301 cgcgtgcatg gatctgccca cgtccatcag cgtcgtcttg gtggcaagca gaacccttga
      361 aatcttcatc gtcaggtcgt tttccgcttt ccggcctttc caggtcggtc ccgttcgggc
      421 ttttccttct cgctcatcgc cttgaccgca gcgtcggtcg cggcgaaaac cgaacctttt
      481 tcccaggttc cgtcttcctg cctggttccg gccggcaagc cggtcatgat ttcgaccact
      541 tcctcgatgc ggcgagccgc ccagatgttg aagcgtcgtt tcctgacggc ctcctcgacc
      601 tccggttcca gaaccaggtt gcgctggttc gcggcaggaa tgatcacacc ctggcgtccg
      661 gtcagtccgc gttcactgca aatcttgaaa aagccctcga tcttctcgtt gacgccaccg
      721 accgcctgca cgtctccggt ctggttcagg cttccggtga cggcgacgtc ctgcctcagc
      781 ggaatccggg aaagcgagga cagaaccgcg cagaattccg cgcatgaagc cgagtccccg
      841 tcgatgcgtc cgtacgactg ttcgaccgtc aggctgaccg tgatgccggg aacgcgattc
      901 tttgcgaaca tgtcgcccag aaggccggaa atgatgagta cggccttgtt gaaggtgttt
      961 ccggaaagcc ttgcctcgcg ttcgacgttg atgattccgg cggaaccccg gaatgatttg
     1021 gcggtgatcc tcaggggcat cccgaaagtc tggtcggcca tctgataaac ggcaagtccg
     1081 ttgatgacgc caggaatcgc gccctcggaa tcgatgatga cggttccgtt cagaaccatc
     1141 tcgacgccct tgtcccagga gagcgaaacg cgatccttgc gctggcggcg cgcggcgatg
     1201 atgtgcctgg cgtcgatgat tgccgatcct tcctggcgtg ccaggtgatc cgcctcgatg
     1261 agcaggttca gaaccagatt gaactgaagc gtgagtctgt cggacgcgtc ggccatccgc
     1321 atggagtatt cgatcacgtc cgcgacgccg gccgacgaaa cgggaaggag tccgtcacgt
     1381 tcactggttg tggctatgaa ccttgcatat tcctggagca tgtcgtcgga aacggcgact
     1441 tccgagtcga actccgcctt gatctggaag gcttttccaa attcctcgtc tgcctcgtgc
     1501 agaagctggt acaggaaatt gctgcctacc aggattacct tgcaggacat cgggatcgga
     1561 ttcggccgga tggtcgtggt aggcaggaac tggtatgcct cgcccatctc ggcaatctcc
     1621 agcagccttt gacgcagcac ggtcttcaag gcctcccacg caaacggata cgacaccagt
     1681 tcccgggtat gcagaatcag gaatccgccg tttgcccgaa gcagagagcc cgccttgatc
     1741 atcgtgaagt cggttgaata gatgccgttt tcaacgcgct tctcgatgcg cccgacaagg
     1801 ttgtggtaag tcggggtgtt ttcgaagacg accggcgcgc ccctgacttc ggagttgtcc
     1861 accagcaggt tgaccttgta ttcggtcatt gccgcttcga tgagttgttt ggtcatgccg
     1921 tcgtcggagt cctctgccag gaatcgcggg atgttttcaa gaatgctggc cctgacagcc
     1981 tccaggtggg ctatcacttc cggacaagat gagtattcgg ccagcagggc cttcatcgac
     2041 tcatccatgg tttcggcaca caggcggttg ttggccacct gaaccttcgc atggacaatc
     2101 tgctccgctt ccttgttctt ctcgaaaaac gccgcgattg caggctcgat tgccctgcgg
     2161 ttttcatcga tggtcttctt ctgggcgtcc gaaagcttcg cgtattcctt gttgctgatc
     2221 gcctcgccgt caaccacggg tatggtgacc aggccttcct tcgtctgctt cacctggaag
     2281 ttgttcctgc ctgccacctc gtccagccgt tcgaaggcct cgttttcgat ggccagtccc
     2341 tcgttcagga tttcctgaag cgtttcctgg tgtttctggg tgtgaaaggc cctgggcagg
     2401 ttcttcttca gcgaagaaat gaactcgtcc atctgaccgc ggaactgaag ccccttgcct
     2461 gccggcaggc tgaaaaccct tggggtttcg gggtgcttga aattattgac catgcaccag
     2521 tcggggggcg tgggcatctg cctggatgcc tcatcgacaa gtcttttgag aaggctcgac
     2581 ttgccggttc cggaggcacc tgaaacgtag atgttgaagt tccgcttctg gatcgaaagg
     2641 ccgagttcga gagctttctg ggcccgggcc tgctcaacga ccccgacaag cttctcgacg
     2701 tgccttgtgc tccggaactt gaatatggaa gggttgatgt ggcgatgtac ctgcttccag
     2761 cccaggcggc agtctttttc agcggacggt tttttcgcgg ccatcgtctg gctccagaac
     2821 ggtttcgcga aagcctatca cgattgacgg aaagcggggg actctcaaag tttgccttcc
     2881 gggggatcac cttgcggagc cctgacggag ttcaggtgga aggtatcgcg aaaaggcccc
     2941 ggcaccggag ttggaaccga agcttgcccc cgattccagc ccgcttccgg cggtggcctt
     3001 gacagcgacc ccgccgcggg aggcgtcggt gaccgtccag ttcctgccgc cgatgatgac
     3061 gtcggcaccg gcggccgtcc tggccgacac catgccgacg gtgcgccctg tccccgcatc
     3121 cacgactttg atcgaggcgg agtcggcgat attggagtgt atgcgtcccc gttcgcccat
     3181 gtccatcaat cttgacgagg cccgaagcac ttcgccggcg ctttcgtcca ggaagccttc
     3241 ggtacgcagg tgatcgacga tcgcgtcgat gaccgggcca ggtgcgacgg ggtcaaggat
     3301 tcttcggatc gtggcgcgtc gaagtccctt tctgttttca aacagcaggg aaaatatctg
     3361 ctgaaccgcg accgacaggt ctggcagcct ttctgactgc gggaaggcct gttcgacgac
     3421 gtcattcatc agcatcgcga attttccttc atccccaggt ccgtccggaa cgaatatggc
     3481 cctcatgccg tcgcgccgcc tgcaaccgcg gccgaccctc tgctggagcg atgcgaccgt
     3541 ttcgggcgct ccgaacagaa cgactgcatc gacgtcaccg atatcgaccc cgagttccat
     3601 ggtggatgtc gagaccagga tgcctgcctc gaagtacctg aaagcctttt caacgtcctc
     3661 gcgttcccgg cgggcgaggc tggcgtggtg caccatgact ctctcggccg gccacgggcc
     3721 cgtcctgagc accagggccg cggtttcctc ggtcttcttc ctggtgttgc agaatattat
     3781 ggccttcgtg agccgttccg aac
//
LOCUS       DAOPPA010000320         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26483_length_3766_cov_5.131770, whole genome shotgun sequence.
ACCESSION   DAOPPA010000320 DAOPPA010000000
VERSION     DAOPPA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26483_length_3766_cov_5.131770"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKH54_09840"
     CDS             complement(<1..234)
                     /locus_tag="PKH54_09840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOD00238.1"
                     /translation="MMNNLDFLLEVGTEEIPARMLARAAQDLEGLLLSALDAAGLAHQ
                     GSERFFTPRRLAVLVRGVPEAQADRSELVPGPSV"
     gene            complement(268..1134)
                     /locus_tag="PKH54_09845"
     CDS             complement(268..1134)
                     /locus_tag="PKH54_09845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOD00239.1"
                     /translation="MTFQEVILRLQDFWAKKGCLIIQPYDLEKGAGTFNPATFLAALG
                     PKPWNAAYVEPSRRPTDGRYGDNPFRLQHYYQFQVIMKPAPENIQQLYLDSLYELGIN
                     PREHDIRFVEDDWESPTLGAWGLGWEVWVDGMEVSQFTYFQQCGGFDLNPIPVELTYG
                     LERITQYLTGIDNVFDLEWGHGVSYRDVAHRQEVEFSTYNFELADVSLHLDLFNKYEA
                     ECLRLLEHGLALPAYDYCMKSSHAFNVLDARGAISVSERQAYIGRVRAMARGCATAWM
                     KTQGFLNPEKAS"
     gene            complement(1279..1689)
                     /locus_tag="PKH54_09850"
     CDS             complement(1279..1689)
                     /locus_tag="PKH54_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00240.1"
                     /translation="MHRVWILSALAAVIAFIGCGREKCLDSTDCEGGFVCRTDRCVVL
                     ECIGQDTCADDEVCAAGRFIDVDPSKAFCTARCSVFNRCPEGRHCSDGLCLASGEDTD
                     GNYVNKSTENPGNACSICKQVSFEPAEIVGYTAD"
     gene            1714..1842
                     /locus_tag="PKH54_09855"
     CDS             1714..1842
                     /locus_tag="PKH54_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00241.1"
                     /translation="MESIKNGHNNGHYGLQPDEMISAANLIPDKQDFQMPSWLGFT"
     gene            1881..3260
                     /locus_tag="PKH54_09860"
     CDS             1881..3260
                     /locus_tag="PKH54_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD00242.1"
                     /translation="MGVVENIVRAVGPRAVVLTDPAAQAGMRSDQSGIVGPLPLCTVR
                     PGDAEDVRLVVSVCRDDGVPIVARGGGTGLEGGCVPVLDCVVIDTSSLDAILRISPED
                     RLATVGAGVTAERLNSLVAEHGLFFPVAPAGAAESATMGGMVSTNARGMHAVKYGSAG
                     DNLLAATVVMADGTLARFGHDVFHSSSGLRMAALLAGAEGILGVITEVTVRLHPLPEA
                     RKSILLEFDNLEDAADVCALIAAYVPDAAAIEIVSAPTISVLLSAFPDAGFPPAGESL
                     VMIELHGGHEAVEHAADTVLDIAADHDGRPFVPPRGTDPWADRHRFTRAISAMSCEGR
                     PCRFDAAVPLTRLTEYIRCLEQAAASVPGRPTTCIFGHAGAGIMHVLLPIGDKPGLWG
                     TAAAAAFRLASTKTALSLGGTASGEHGIGLSSIGPCAAENAASIHWMRAVRLALDPQE
                     IMNPGKVLL"
     gene            complement(3275..>3766)
                     /locus_tag="PKH54_09865"
     CDS             complement(3275..>3766)
                     /locus_tag="PKH54_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00243.1"
                     /translation="ESIPCEDPEVDWSKVFGGTHVSLYARLGPAFSDLARTLLNPDLV
                     QSDTVGIDNGIDRRAVFYLPAGDGAPNCYEYMPLCSIAAQMSHAMKDSSQNPMAEIVK
                     SVECMNSVAVGDLPVCRVFNEYEFQSCSQGLCCQGQCLSKPDDENIWELDENCNWTTK
                     APD"
BASE COUNT          786 a         1140 c         1094 g          746 t
ORIGIN      
        1 gacggacggg ccgggaacga gttcgctccg gtcggcctgc gcttccggaa cgccgcgcac
       61 cagaaccgcc agacgccgcg gcgtaaagaa tcgttccgat ccctgatgtg caagacccgc
      121 cgcatccagc gccgacaaaa gcagcccttc aagatcctgg gcggcgcggg cgagcatcct
      181 ggccgggatt tcttcggttc cgacttcaag caggaaatcg aggttgttca tcattcaccg
      241 ccacgggcca aatggccatg ggccccgtca ggaagccttt tcaggattca ggaacccctg
      301 tgtcttcatc caggccgtcg cgcagcccct ggccattgcc cggacacgtc cgatataagc
      361 ctggcgctcg gaaaccgaga tggcgccgcg ggcatcaagg acgttgaagg catggctgct
      421 tttcatgcag tagtcatagg ctggcagcgc cagcccgtgc tccagcagac gcaggcattc
      481 cgcctcgtat ttgttgaaaa gatccaggtg cagcgacacg tcggcaagct caaagttgta
      541 agtcgagaat tcgacttcct gacggtgcgc gacatccctg tacgaaacgc cgtgccccca
      601 ttcaagatca aaaacgttgt cgattccggt caggtactgg gtaatccgct cgagcccata
      661 agtcagctcg accggaatcg gattcaggtc gaaaccgccg cactgctgga agtaggtgaa
      721 ctgactgact tccatgccat cgacccacac ttcccagccc aggccccagg ccccgagggt
      781 cggtgattcc cagtcatcct ccacgaaacg gatatcgtgt tcacgtggat tgatgccaag
      841 ctcgtaaaga ctgtccaggt atagctgctg aatattttcc ggggccggct tcatgattac
      901 ctgaaactgg tagtaatgct gaagcctgaa aggattgtcg ccgtaacgac cgtcggtcgg
      961 gcggcgcgat ggctcgacat aagcggcgtt ccagggcttc gggccaagcg cggccagaaa
     1021 agtcgccgga ttgaacgtac ccgcgccctt ttccaggtca tacggctgaa tgatcagaca
     1081 gcccttcttc gcccagaagt cctgcaaccg caaaattact tcctgaaaag tcatcaagca
     1141 cctcccaagg cgcgcggtac tctagcagat gtcacggggg ctaacaatcg gtaatgtggc
     1201 tgggcgatca gggcgggctc gcaaggcaag cccgggaatc cgccaatccc cgaccaccgg
     1261 cagcggctgg tgacgtgatc agtccgccgt gtagccgaca atctcagcgg gctcgaaact
     1321 cacctgcttg caaatcgaac aggcgtttcc aggattctcc gtgctcttat tcacgtagtt
     1381 gccgtcggta tcttcaccag aagccaggca caatccatcg gaacaatgtc tgccttccgg
     1441 gcagcggttg aacacgctgc acctggcggt acagaaagct tttgagggat cgacatcgat
     1501 gaaacgccct gcggcgcaga cctcatcgtc cgcacaggtg tcctgtccga tacactccag
     1561 aacgacacag cgatccgttc ggcacacgaa gccgccctcg caatcggttg aatccaggca
     1621 cttctcccgg ccgcaaccga taaacgcgat gacggcagcc agggccgaca gaatccagac
     1681 ccgatgcatg ttgctgcaaa cccgggggaa tgaatggaat cgatcaaaaa cggtcataac
     1741 aacggccatt atggcctcca gccggatgaa atgatttctg ccgcgaatct tattcccgac
     1801 aaacaggact ttcaaatgcc atcgtggttg ggcttcacct gaggcacgat tcggataaga
     1861 ttcggccatg aggtggcttc atgggcgttg ttgaaaacat tgtccgcgcg gtcgggccac
     1921 gggcggtcgt gctgaccgat ccggctgccc aggctggaat gcgttcggat cagtcgggta
     1981 ttgtcggacc cttgccgctg tgcacggtca ggccgggtga cgccgaggat gtgcggcttg
     2041 tcgtgtcggt gtgtcgtgac gatggcgtcc caatcgtcgc caggggcggt ggaaccggcc
     2101 tcgagggtgg atgcgtcccc gtcctggatt gcgtcgttat cgacacgtcc agccttgacg
     2161 cgatcctccg cataagcccg gaggatcgcc ttgcgacggt gggagccggc gtcaccgcgg
     2221 aaagactgaa cagcctggtc gccgaacatg gactgttctt tccggtggcc cccgccggtg
     2281 cggcggaaag cgcgaccatg ggtggcatgg tcagcacgaa cgcgcgcggg atgcacgcgg
     2341 tcaagtacgg aagcgcgggg gataacctgc ttgccgcgac cgtggttatg gcggatggaa
     2401 ccctcgcccg cttcggacac gacgtttttc acagcagcag cggactgcgc atggcagccc
     2461 tgcttgccgg ggccgaaggc attctgggcg taatcaccga agtcacggtt cgccttcacc
     2521 ccctgccgga agctcgcaaa tcgattcttc ttgaattcga taaccttgag gacgccgccg
     2581 acgtctgcgc cctgattgcg gcatatgtcc cggacgcggc cgccattgaa attgtttcgg
     2641 cgcccacaat ttccgttctt ctgtcggcgt ttcccgacgc cggcttcccg cccgcgggcg
     2701 agtcgcttgt aatgattgag cttcatggcg gccacgaggc tgtcgaacat gccgccgaca
     2761 cggttcttga tatcgccgcc gaccatgatg ggcgcccttt cgtgccacca cgtggaacgg
     2821 atccctgggc agaccgccac cggttcacaa gggcgatttc cgcgatgtct tgcgaaggaa
     2881 gaccctgtcg gttcgacgcc gcggttcccc tgacccgcct taccgaatac attcgttgcc
     2941 ttgaacaagc ggccgcttcg gttccgggcc gtccgaccac atgcattttt gggcacgcgg
     3001 gggccggtat tatgcacgta ctgcttccga tcggggacaa accggggcta tggggcactg
     3061 cggccgcggc ggcctttcgg ttggcctcca cgaaaacggc actgtccctg ggcggcacgg
     3121 caagcggcga gcacggaatc ggtctttcat caatcgggcc ctgcgccgcg gaaaatgccg
     3181 cttcaattca ctggatgcgc gccgtcaggc tggcactgga tccccaggaa ataatgaatc
     3241 cgggcaaggt tcttctttaa ttgaaaccga aagattagtc tggagccttt gtcgtccagt
     3301 tgcagttctc atcaagctcc cagatgttct cgtcatcagg cttggaaagg cactgaccct
     3361 gacagcagag tccctgggaa catgactgga attcatactc gttaaaaacc cgacacacag
     3421 gcagatcgcc gacggccacg gaattcatgc actcgaccga tttaactatt tccgccatgg
     3481 gattctgact tgaatccttc atggcgtggg acatctgggc ggcaatcgaa caaagcggca
     3541 tgtactcgta gcagttcggg gcgccatccc cggccggaag ataaaatacc gcgcggcggt
     3601 cgatgccgtt atcgattcca acggtatcgg attgaaccag atccggattc agaagcgttc
     3661 ttgccaaatc actgaaggca ggccccagcc ttgcataaag ggaaacatgg gttccaccga
     3721 aaaccttgct ccagtcaacc tcgggatcct cgcacggaat ggattc
//
LOCUS       DAOPPA010000321         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26572_length_3757_cov_4.987034, whole genome shotgun sequence.
ACCESSION   DAOPPA010000321 DAOPPA010000000
VERSION     DAOPPA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26572_length_3757_cov_4.987034"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKH54_09870"
     CDS             <1..280
                     /locus_tag="PKH54_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00244.1"
                     /translation="WEDFTLMSVRYLPGRKTGEEEAIAVVKASVRRDNSITIEKKQYR
                     EHWKVRNRKWILVKSEILDTKQMGANSDIGKNDIDGDDVNPVKPGEKD"
     gene            314..1432
                     /locus_tag="PKH54_09875"
     CDS             314..1432
                     /locus_tag="PKH54_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD00245.1"
                     /translation="MGTARRKFNTGLKWAVSVAIGGLFVWLAFRDWPVESIAAGGIRI
                     ADGMITTDGWRVSFLTLFLYFLTLLSLHLFRFWRWQPLLKPLGHVDFWTLNRVCSVGN
                     MAVFLLPMRLGELVRPMLVSTEAPIRRSAALATIVVERVVDGIIVAGFLAVALLFMPK
                     SNLDSYLEIRIGTYITLVIFGVAVTVLILMFAFRKRLSVVIHGMAGRFPGTGAASRIA
                     GMVDRFMFGLSVFPDFKNFLLFLLMSLAYWCSNGFGLFLLASGFQVNSSAGWVPLGVP
                     LIGAFAMMSTIVVGMMIPNAPANIGSFWYFLLKPMELYGIEAGNPAGLAYALTVWTLQ
                     LLQLLIFGGWFILKGQVSFRKAFAIGLNDIEGTGGERN"
     gene            1434..2240
                     /locus_tag="PKH54_09880"
     CDS             1434..2240
                     /locus_tag="PKH54_09880"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOD00246.1"
                     /translation="MTRGRPVWPTAFVLVFAVALCAQGAARADTFYECVGKDGVKGFF
                     NQKQPGQKCSVYLDFNAQPSKPKGSKCVVQRYRNSVFYKCEKNGVWYIFNRTEDEQSG
                     SGGRQDSAASGGGSSAQVRRVVSSEEIVPGPSKRGPERVEGLDRMVKAAAAEFGIPEA
                     LLYAVIEVESGFNPDVVSPVGAQGLMQLMPVTADYLNLDNPFDPEQNVRAGAKLLRLL
                     SDRFDGNHELVIAAYYTGAGNVQKAGNVVPRSCENYVARVMERYRKHLSK"
     gene            complement(2206..>3757)
                     /locus_tag="PKH54_09885"
     CDS             complement(2206..>3757)
                     /locus_tag="PKH54_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00247.1"
                     /translation="ALPPFISGRWFRAIASAAAQGHIGRLAALTLRFGPAEHESSQDP
                     DSPAARAALVVSGLTGRRIRFNASRSGASGSPALATRLEGSIDGIDVPVSFEINPAPA
                     PLVVEVVGVSGRIIAVLDGSGADVILESGSGRDTIFHGRLNPFDDAASAIVAGLSRNS
                     TTFFPLSTVNSALDSLAAMSAAAFSTSLPDIRTGGIRTSFIANDFTSSDDVSTGSGTI
                     RELKVFVSDACNLNCPFCFSRSPEKAAPSVDSFRSVFKDAVAAGYDSALLSGGEPTLN
                     PDLRDIVVAARNAGMRFVGIETNAILLDTQGAAAELASAGLDRALVSFHSMDPETLVA
                     LTGSRTSLPRAIGGIRALLDAGIQVNINCVTTALNYRELPEITTFLSGLRPAISQMVL
                     SFMAGLGSATDHPELYPRVSDAAPFLRRAIEIASSSGIDAVIPGQCGLPQCVLPDKLD
                     RFCNQQLATASPSWTAPALEGKVFAASCTECRVRRWCYGVWKVYADRHGLEEFRPITS
                     TGVSGSAP"
BASE COUNT          749 a         1037 c         1215 g          756 t
ORIGIN      
        1 gtgggaagat ttcacactga tgtcggtgcg ctatcttccc gggcgcaaga cgggcgagga
       61 ggaggccatt gcagtggtca aggctagcgt tcgccgggac aactcgatca cgattgaaaa
      121 gaagcagtac cgcgaacact ggaaggttcg caaccgcaag tggatcctgg tcaagtccga
      181 gattctcgac acgaagcaga tgggcgcaaa cagcgacatc ggcaagaacg acattgacgg
      241 cgatgacgtg aatccggtta agcccgggga gaaggattga ccggagccgg gcgccacgcc
      301 ttgactggtg aagatgggca ctgccagaag gaaattcaat acgggtttga agtgggcggt
      361 ttcggtcgcc ataggtggtc ttttcgtgtg gctggcattc cgggactggc cggtggaatc
      421 aatcgccgcc ggggggatcc gaattgccga cggaatgatt accaccgacg gctggcgcgt
      481 atcgttcctg actctgttcc tttatttcct gactcttctt tccttgcacc tgttcaggtt
      541 ctggcggtgg cagcctttgc tgaaaccgtt gggacacgtc gatttctgga ccctgaaccg
      601 ggtctgttcc gtcggaaaca tggcggtgtt cctgcttccg atgcgtcttg gcgaactggt
      661 tcgtccgatg cttgtatcca ccgaggcacc catccgaaga tcggccgcgc tggcgacgat
      721 cgttgttgaa agggtggtcg atgggatcat cgtcgccgga ttccttgcgg tggcactgct
      781 cttcatgccg aagtcgaacc tggattcata tctggaaatc aggatcggaa cgtatatcac
      841 gctcgtgatt ttcggtgtgg cggtcaccgt ccttatcctg atgtttgcct tccgcaagag
      901 gctgtcggtc gtgatccacg gcatggccgg caggtttccg ggaaccgggg cggcaagcag
      961 gattgccggg atggtcgatc gattcatgtt cggactgtcg gtatttcccg acttcaagaa
     1021 ctttcttctg tttcttctga tgtcgcttgc ctactggtgc tcgaacggct tcgggctgtt
     1081 cctgctggca agcggtttcc aggtgaattc gagcgcgggt tgggttccgc tgggcgtgcc
     1141 cctgatcgga gcgttcgcga tgatgtcaac gatcgtggtc gggatgatga ttccgaatgc
     1201 tccggccaac atcgggtctt tctggtactt tcttctgaag ccgatggagc tttacggcat
     1261 cgaggcggga aaccccgcgg gcctggccta tgcgcttacg gtgtggacgc ttcaattgct
     1321 ccagctcctg atttttggcg gatggttcat cctgaagggc caggtttcct tccggaaggc
     1381 gtttgcaatc ggattgaacg acattgaggg caccggtggg gaaaggaact gacatgaccc
     1441 gggggagacc ggtctggccg acggccttcg tcctcgtctt cgcggtcgcc ttgtgcgcgc
     1501 agggcgcggc gcgggcagat accttctacg agtgcgtcgg caaggacggc gtgaagggct
     1561 ttttcaacca gaagcagccc ggccagaagt gctcggtcta tcttgatttc aacgcccagc
     1621 cgtcaaaacc gaagggctcg aagtgcgtcg tgcagagata ccggaactcg gtcttttaca
     1681 agtgcgaaaa gaatggcgtc tggtacatat tcaaccgcac cgaggacgaa cagtcaggca
     1741 gcggcgggcg gcaggattcg gctgcgtccg gcggcggttc gtcggcccag gttcgtcgcg
     1801 tcgtttcatc cgaggagatc gtgcccggtc cttcaaagcg tggcccggaa agggtggagg
     1861 gactcgatcg gatggtgaag gccgccgccg ccgaattcgg aattccggag gcgcttctgt
     1921 acgcggtaat cgaggtcgaa tccgggttca atcccgatgt ggtctcgcct gtcggcgccc
     1981 agggcctgat gcagttgatg ccggttaccg ccgattatct gaatctggac aacccgttcg
     2041 atcctgaaca gaacgtcagg gccggcgcca agctgctgcg tcttctttcg gatcgtttcg
     2101 acggcaatca tgaacttgtg atcgcggcgt actacaccgg cgcaggcaac gtgcagaagg
     2161 ccggcaacgt cgtcccgagg tcatgtgaaa actatgtggc cagggtcatg gagcgctacc
     2221 ggaaacacct gtcgaagtga tcggcctgaa ttcctccagt ccgtgcctgt ccgcatatac
     2281 cttccagact ccgtaacacc agcggcgaac acgacattcg gtgcaggaag ctgcaaagac
     2341 ctttccttcc agcgccggcg ccgtccagga tggggatgcc gtcgcgagct gctggttgca
     2401 gaaccgatct agcttgtcgg gaagcacgca ctggggaagg ccgcactggc cagggatgac
     2461 cgcgtcgata ccggaggacg aggcgatttc gatggcccgc ctgaggaatg gagccgcgtc
     2521 tgaaacccgc ggatagagtt cggggtgatc cgtggccgaa ccgaggccgg ccatgaagct
     2581 gaggaccatc tggctgattg ccggacgcag cccggaaagg aatgtcgtga tttccggaag
     2641 ctcccggtaa ttcaacgcgg tggtcacgca gttgatgttg acctggattc cggcatcaag
     2701 aagagcgcgg ataccaccga tggcccttgg cagcgacgtg cgcgagccgg tgagcgcgac
     2761 aagcgtttcc ggatccatcg aatgaaagga gacaagtgcc ctgtcaagtc ctgccgatgc
     2821 gagttcggcg gccgccccct gcgtgtccag aagaattgcg ttcgtttcga tccccacgaa
     2881 acgcattccc gcgttccggg cggcgacgac gatatcgcga agatccggat tgagtgtcgg
     2941 ctcgccgccg gaaagcaggg cggaatcata cccggccgcg acggcgtcct tgaagacgga
     3001 ccggaagcta tcgaccgaag gggctgcctt ttcgggcgac cgggagaaac agaacggaca
     3061 gttcagattg catgcgtccg aaacgaagac cttcaattca cgaatcgtcc cggaaccggt
     3121 tgagacgtcg tcggacgaag tgaagtcgtt cgcgatgaag ctcgtccgga taccccccgt
     3181 ccggatatcc ggtaatgacg tcgagaatgc ggcggcagac atcgcggcaa gtgaatcaag
     3241 ggccgagttg acggttgaaa gcggaaaaaa tgtcgtcgag ttcctggaca agccggcaac
     3301 gatcgccgat gccgcgtcat cgaacggatt caggcgtccg tgaaagatcg tgtcgcggcc
     3361 ggatcccgat tcgagaatca cgtcggcgcc cgaaccgtcg agaaccgcga tgatcctgcc
     3421 ggatactccc acgacttcca ccaccagggg ggcaggcgcc ggattgatct caaaggacac
     3481 cggaacatca atcccgtcga tggagccttc aagcctggtg gccagcgcgg gtgaaccgga
     3541 ggcgcctgac cgggatgcat tgaaacgaat ccgccgtccc gtcagtccgc tgacgacaag
     3601 cgccgcgcgg gctgccggag aatccggatc ctgggatgat tcgtgttcgg ccggaccgaa
     3661 acggagggta agtgccgcca gcctgccgat gtgcccttga gccgcggccg aggcgatggc
     3721 gcggaaccag cggcccgaaa tgaatggtgg aagcgcg
//
LOCUS       DAOPPA010000322         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26750_length_3735_cov_4.867935, whole genome shotgun sequence.
ACCESSION   DAOPPA010000322 DAOPPA010000000
VERSION     DAOPPA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26750_length_3735_cov_4.867935"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PKH54_09890"
     CDS             complement(<1..1825)
                     /locus_tag="PKH54_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00248.1"
                     /translation="MNLRISFLMLAVALAVIPSGAPAQSAEPKPVVAVFDIAIDGFKL
                     SEVTMKRLTKYLGVRLMMGGLYQTAPESQLKDAMVALKLESQKDCYDERCRVQLGLEA
                     AANKNLNVTIWKVGGKCSIIGALYDLRAAASEEAAEVADLNCDEEGLKAGIARFAAQL
                     SGRKGPTPSGPTEMPGALPPPPEVQGGGGGFGGGDAPPPVKAEAGYLSVEGTPKGARV
                     DISGPKEFGSNGKSATSLPLRPIPVPAGEYRVSVSMTGYDTEEKSVRVYADATEIVKF
                     DLMLSSGQIQISGKPDGAKSRLECQKGFVRDFGLPAPASPWTVSVPRGQCRLVVERDG
                     YEKYEQTFEVAGGATVRQSVELKRGFAGMAGWVRIPAGTFVMGSPSSESGRYDDEGPQ
                     TRVTISRSFLLKATEVTQGEWKAVMGTSPSSFKNCGDNCPVENVSWFDAVEFCNRLSS
                     REGLEQCYRINGTTVVSKGPSCGGYRLPTEAEWEYAARAGTTTALYTGGLTIRGDNNG
                     PELDPIAWYGGNSGVSYSGGYDCSGWSAKQYSSSSCGTHPVGGKRPNQWGLYDMLGNV
                     LEWCDDWKDNYSGGSVTDPTGPSSGSARVVRGGSWYCDARNV"
     gene            complement(2095..3498)
                     /locus_tag="PKH54_09895"
     CDS             complement(2095..3498)
                     /locus_tag="PKH54_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00249.1"
                     /translation="MDPRFLAHNSHLEGVGSFHLMDPHLRYLSRQPFVFQSPLLDQLP
                     VDTPGVYTVSGGRQIGKTTALKQWMRRLMHDGVNPVDIAFFTGELIDDHHTLVNLVTA
                     HLQNRTSCQYVIVDEVGYIRDWDKGIKFLADAGYLMNVELVLTGSDSVLIQEARLRFP
                     GRRGNAEKVDFVVTPLSFRQAALLKNTIAPEEAVLMDAAPGGNTVDDGGIHGQPLPPG
                     LVSRLFQEFERYMAHGGFLTAINDLQINGSILPATLRTYSDWIRGDFVKRGRQEAFLK
                     EILASVLRHQGSQLSWHNLARDMSIEHPQTVADYVALLASMDVLLIQPALLEDKLVEA
                     PRKPRKLHFRDPFIRHAVRYWLDPSGDPYHDGILADVSDSVASSVLAESVAVSHVARN
                     FPTFYIKAAGEVDIAWVEGSRFFPVEVKWTGQLRSKELQQVRKYPNARIWSRQPEYFM
                     AGEIPVEPLPLALWRMG"
BASE COUNT          837 a         1199 c          954 g          745 t
ORIGIN      
        1 gaacgttcct ggcgtcgcag taccacgagc cgccgcgaac aacccgggcg gagcccgatg
       61 aaggaccggt cggatccgtc accgaaccgc ctgaataatt gtccttccag tcgtcgcacc
      121 attccaagac attgccaagc atgtcataca aaccccactg attcggccgt ttgccaccca
      181 ctggatgcgt gccgcaactt gacgacgaat actgcttggc tgaccagccc gaacaatcat
      241 aaccgcccga atagctgaca ccgctgttgc cgccgtacca cgcaatcgga tcaagttccg
      301 ggccgttgtt atcaccacga atcgtcaatc cacccgtgta aagggccgtg gtcgtccccg
      361 cgcgcgccgc gtattcccat tccgcttcgg tcggaagacg atacccacca cacgacggcc
      421 ctttcgatac aaccgtcgtg ccgtttatcc gataacactg ttccaggcct tcacgcgacg
      481 ataaccggtt gcagaactcg accgcatcga accagcttac attctcaacc ggacagttat
      541 cgccgcagtt cttgaaactg gatggggatg tccccatcac agccttccac tcgccctgcg
      601 tcacctcggt tgccttcaac agaaatgatc tgcttatagt cacacgcgtc tgcggccctt
      661 catcgtcgta ccggccagac tcgctggacg gcgaacccat cacgaacgtc cccgccggta
      721 tccgcaccca gcccgccatc ccggcaaaac cacgcttcag ttccaccgac tggcgaaccg
      781 tcgcgccgcc cgcgacctcg aacgtctgtt catacttttc gtatccgtcg cgttccacca
      841 caaggcggca ctggcctcgc ggcacggata cggtccacgg acttgcgggc gccggaagcc
      901 cgaaatcacg cacaaatccc ttctgacatt ccaggcggct ttttgcgccg tccggcttgc
      961 ccgatatctg gatctggcca gacgacaaca tcagatcaaa cttcacaatt tccgtcgcat
     1021 cggcatatac ccgcaccgac ttttcttccg tgtcgtagcc cgtcatgctg acagatacgc
     1081 ggtattcacc ggcaggcacc ggaattggac gcagcggaag cgacgtcgca gacttgccgt
     1141 ttgacccaaa ttccttcggc ccgctgatat cgacccgcgc acccttcgga gtaccttcaa
     1201 cagacaggta cccggcctcg gccttcaccg gtggcggcgc gtcaccacca ccgaagccgc
     1261 caccgccacc ctgaacttcc ggaggcggcg gaagcgcccc gggcatttca gtcggccctg
     1321 acggcgttgg ccccttccgc ccgcttaact gcgcggcaaa gcgggcgata ccggccttca
     1381 ggccttcctc gtcgcaattc aggtcggcga cttcggcggc ctcctcggac gcggcggcac
     1441 gcaaatcgta aagcgcgcct ataatcgagc acttgccgcc gaccttccag atcgtcacgt
     1501 taaggttctt gttggctgcc gcttcaaggc caagctgaac acggcatctt tcgtcgtaac
     1561 aatccttctg cgattccagc ttcaacgcca ccatcgcatc cttcaactga ctttccggag
     1621 ccgtctggta aaggccgccc atcatcaggc gcaccccaag gtatttggtc aggcgcttca
     1681 tcgtgacttc ggacagcttg aaaccgtcga tcgcgatgtc gaacaccgcc accacaggct
     1741 tcggttcggc cgactgggcg ggcgccccgg acggaatcac ggccagagcc acggcaagca
     1801 tcaggaagga aattcgcagg ttcatcggca acctcttcga caggaaactg ggtcggacga
     1861 tgttttatac cataaatgga ttcgcagcgg acgatcgtaa tccgcccata catgtacggg
     1921 cgaatgaaaa ttcgccatcc ccagcaaccc gatccgtacg ggcgaatgaa aattcgcccg
     1981 cttaaacgat gcggggcacc gtgcttgttc gcagcgggct gatgtaatcc gcccgtacag
     2041 ggcacgaact ggcgaacgat tgtaatccgc ccatgcaagg cacgaacagt cggactagcc
     2101 catgcgccaa agcgccaggg gcagcggctc gaccggaatt tcaccggcca tgaagtattc
     2161 cggctggcgc gaccagatcc gggcatttgg atactttctt acctgctgca gttcctttga
     2221 gcgcaattgc ccggtccact tcacctcgac gggaaagaat cgacttccct ccacccaggc
     2281 gatatcgacc tcgccggccg ccttgatgta gaacgtcggg aaattccggg ccacgtgcga
     2341 aacggcgaca ctttcggcca gcaccgaaga cgccacggaa tcggagacgt ctgccagaat
     2401 gccgtcgtga taaggatccc ctgacggatc cagccagtat cttaccgcgt gacggatgaa
     2461 cggatccctg aaatgaagct tgcgcggctt ccgcggcgcc tcgaccagct tgtcttccaa
     2521 cagtgccggc tggatcagca gcacgtccat tgatgcaaga agcgcgacat aatccgccac
     2581 agtctgcgga tgttcaatcg acatgtccct ggcaagattg tgccacgaga gctggctgcc
     2641 ctggtgacga aggacggacg ccagaatttc ctttaagaac gcctcctgcc ggcccctttt
     2701 aacgaaatca ccgcgaatcc agtcgctgta cgtccgcaat gttgcgggca atatggaacc
     2761 gttaatttgc aggtcgttga ttgcagtcag aaagccgcca tgagccatat acctttcaaa
     2821 ttcctgaaac agtcttgaaa caagaccggg aggaagcggt tgaccatgaa ttccaccatc
     2881 atcgacagta tttccgcccg gggcggcgtc catcaggacc gcttcttcag gagcaatggt
     2941 gttcttaagc aatgccgcct ggcgaaacga caggggcgtg acaacgaaat cgaccttttc
     3001 cgcattgccc cgccgaccgg gaaatcgcaa ccgcgcttcc tgaatcagca cagaatcgga
     3061 accggtaaga accagttcca cgttcatcag gtatcccgcg tcggcaagga acttgattcc
     3121 cttatcccag tcgcggatat aaccgacttc atccacgatt acatactgac acgaggtccg
     3181 gttttgaagg tgcgccgtaa caaggttgac cagggtgtga tggtcgtcga taagctcgcc
     3241 tgtaaaaaat gcgatatcga ccggattgac gccgtcatgc ataagccggc gcatccactg
     3301 tttcaaggca gtcgtctttc ctatctgacg ccccccggaa accgtatata cgcccggcgt
     3361 atcgacggga agctggtcaa gcagagggga ctgaaacaca aacggctgcc ttgacagata
     3421 ccgaaggtgc ggatccatca agtgaaacga tcccacgcct tcaaggtgtg aattatgtgc
     3481 gagaaatctt ggatccatga ttttgaacat ccatcagtga tcaaatttga tcattcacca
     3541 ttatccaaaa ttcgcttttg ctgtcaacta tttgaactaa ctggctttac tcaggctgcc
     3601 cacgcacatc gcggacaatc cggaagcctg tcacgaagtc gatggtgtca ggagtcacgg
     3661 cgtcccgatt cgcgatttcc agatccttgg ggccggaccg ccacgagccg ccacgaatca
     3721 cgcggtacgc cgaat
//
LOCUS       DAOPPA010000323         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26836_length_3727_cov_3.748638, whole genome shotgun sequence.
ACCESSION   DAOPPA010000323 DAOPPA010000000
VERSION     DAOPPA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26836_length_3727_cov_3.748638"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PKH54_09900"
     CDS             complement(<1..952)
                     /locus_tag="PKH54_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00250.1"
                     /translation="MIVKALKKVLTDQAVMGMVVSDVKVPIQLVVGANLNALEIGQVD
                     MDCLNVPMHLRHDSVPWIPMRLDTMPAQYFGKPGLIVGRNSGVALEYLVEHWVCGQNE
                     NIDPAVKAAREARAKGEPFLHLVQRFAVSESGRRHWDVEDESSEAFVWTKEQFRCSFD
                     PIHDTLRGVYTADRGMFDGEKMQAIVVARTIVVGGHKLCGWDMMVMPDDKDSGAVVVS
                     SESGWQAGPFRRLDPALIQRFDQHGRLVAITQADMTVWQNWVARILVKVPRSTGAMGS
                     EIDPSCLADIPVGKLVDARLPVSIARDVQVVADPAVARDIL"
     gene            complement(949..2952)
                     /locus_tag="PKH54_09905"
     CDS             complement(949..2952)
                     /locus_tag="PKH54_09905"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOD00251.1"
                     /translation="MNKDTCVQRVSFAVSTRNLTVDDIPELVDDTDPEGTTGLDDTPA
                     ADPALETEPASEAEDFPDPDYPEPVEVPEEIARWIEEELRKQKAPAQVDDPIPKEFDG
                     PVINKQAAIQEVPPAPEDSRQADSYPVATPEAPPARPAPDKATPSAGRFDLPPDSRDV
                     PQSDLGDLFNDHDEGNTNIRPDIRFVWDAGTDTWRDHAGRPLSPLKYFRGRSVFSLLG
                     IHGCRAIEDYVIAGMALGEPVLLIGAPGSAKTAMTERLASDMHLRFWAYDASKAMFED
                     IVGFPDPASLGRGEVRYVPTPLSLMGKQFVLVDEVSRAHPALQNKWLEIIRSRRVMGI
                     DLPDLQMVFGAMNPPGLAGTVPLDEALAGRFTFHIQVPDINAMSDGERRSVIEAGDRN
                     PIDSGDRSSRIRWIVRAAREAMAAVETEYGSVITTYVAELSRYLAGRDWKLDGRRQGM
                     IRRGLIAYVATRSLFMEGTPSPGSLSRMLRVGIEGLLPFDAVGRDLPRVILDSAHQYA
                     GAAMAGNVRSLPPSDLLMAADMVARGMVVRDGDQLSMLVTRILQTLDRAGRTEERIKA
                     GAALLILGLSPEAMRHLPIEARQRVIVALADSLELESDKVGDFLDESFKLTVEGLEPQ
                     LQEPVARVSYNIIRRRGRHRGNTMEFEQAVTLMTRYIMEGGVA"
     gene            complement(3325..>3727)
                     /locus_tag="PKH54_09910"
     CDS             complement(3325..>3727)
                     /locus_tag="PKH54_09910"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOD00252.1"
                     /translation="YGKSLEWAGDCDVDSVVGVVKAASSRRPQDYQSQQGWVLIAFQN
                     ALWQLLHTTSLEEGIVDTIMQGGDTDTNACICGALLGAVHGESAVPGRWLQSLQSCRP
                     EAGRPGVRRPRPRTFWPCDALDLADALIGAG"
BASE COUNT          729 a         1201 c         1082 g          715 t
ORIGIN      
        1 acaggatgtc ccgggccacc gccggatcgg caaccacctg gacgtccctg gcaatgctga
       61 cgggcaacct ggcatccacc agcttgccga caggaatgtc cgcgaggcag gatgggtcga
      121 tttcactgcc catggcgccg gttgaccgcg gaaccttcac aaggattctg gcaacccagt
      181 tctgccagac ggtcatatcc gcctgcgtta tcgcaaccag ccggccatgc tggtcgaagc
      241 gctgaatcag ggcgggatcc aacctgcgga atggacccgc ctgccaaccg ctttccgaag
      301 aaacaaccac cgccccggaa tccttgtcgt cgggcatcac catcatgtcc cagccgcaca
      361 gcttgtggcc gccgacaacg atggtccttg caacgactat cgcctgcatc ttctcgccgt
      421 cgaacatgcc ccggtcggcg gtatagacgc cgcgaagcgt gtcgtgtatc gggtcaaagg
      481 aacaccggaa ctgctccttg gtccagacga acgcttcaga ggactcgtct tcaacatccc
      541 agtgacggcg cccgctttcg gacacggcga aacgctgaac cagatgcagg aacggctcac
      601 ctttcgcgcg tgcttcacgc gcggccttca ctgcggggtc gatattctcg ttctgtccgc
      661 acacccagtg ctcgaccagg tattcaaggg cgacaccgga gttgcggccg acgatcagtc
      721 cgggctttcc aaaatactgg gccggcatcg tgtcaagacg catcggaatc cacgggaccg
      781 agtcgtgccg cagatgcatc ggcacgttca aacagtccat atcgacctgg ccgatttcca
      841 acgcattcaa atttgccccg accaccagct ggatcggaac cttgacatcc gacaccacca
      901 ttcccatcac ggcctggtcc gtcaggactt tcttcaatgc tttcactatc atgcgacacc
      961 tccttccatg atgtacctgg tcatcaaggt cactgcctgc tcgaactcca tcgtatttcc
     1021 ccgatgacgg ccgcgccgac gaatgatgtt gtacgacacc cgtgcaaccg gttcctgcag
     1081 ttgcggttca aggccctcga cggtcagctt gaacgactcg tccagaaaat cgccaacctt
     1141 gtcggactca agttccagcg aatctgccaa ggcaacaatc acacgctgtc gcgcctcgat
     1201 cggcagatgc cgcatcgcct cgggcgacag gcccagaatc agcagggccg cacctgcctt
     1261 gatcctctct tccgtccttc cggcccgatc cagcgtctgc aggatcctgg tcaccagcat
     1321 cgaaagctgg tcgccgtcgc gcacgaccat gcccctggcg accatgtcgg ccgccatcag
     1381 gagatccgac gggggaagag atctgacgtt gccggccatt gccgcaccag cgtattgatg
     1441 cgcgctgtcc aggatgacgc gtggcagatc gcgaccgacc gcatcgaacg gcagaagacc
     1501 ctcgatgccg acacgaagca tccgcgacag gctgccgggc gatggagttc cttccatgaa
     1561 cagggacctt gtcgccacat acgcaatcag gccgcgacga atcatgccct ggcggcggcc
     1621 gtccagtttc cagtcccggc cggccagata ccgggagagt tcggccacat aagtggtgat
     1681 gacactgccg tattccgtct cgacggccgc catcgcttcc cgcgcggcac gaacaatcca
     1741 cctgatgcgc gaagaccggt cgcccgaatc gattggattg cgatcgccgg cctcgattac
     1801 cgaacgcctt tcgccgtcgg acatcgcatt gatatcgggc acctggatgt ggaacgtgaa
     1861 ccttccggca agggcttcgt caagcggcac ggttccggcc aggccgggcg ggttcatggc
     1921 gccgaagacc atctgcagat ccggcaggtc gattcccatc acgcgccgcg accggatgat
     1981 ttccagccac ttgttctgca gcgcggggtg cgcgcggctg acttcatcga ccagcacgaa
     2041 ctgcttgccc atcaacgaca ggggcgtcgg gacatatcgg acttcaccgc gtcccaggct
     2101 ggccgggtcg ggaaaaccga cgatgtcttc aaacatcgcc ttggacgcgt cgtatgccca
     2161 gaagcgcagg tgcatgtcgg atgccaggcg ctcggtcatt gcggtctttg cgctgccggg
     2221 ggcgccgatc agaagaaccg gctcgccaag cgccattcca gcgatgacgt agtcttcgat
     2281 ggcacggcag ccatgaattc caagcaggga aaacaccgat ctgccgcgga aatacttcaa
     2341 cggcgaaagg ggcctgccgg cgtggtcacg ccaggtgtcg gtcccggcgt cccagacgaa
     2401 acggatatcg ggcctgatgt tggtattgcc ttcatcatga tcgttgaaca gatcgcccag
     2461 atcggactgt ggcacgtcgc gcgaatccgg gggaaggtcg aaccggccag ccgaaggggt
     2521 ggctttgtcc ggtgcgggcc tggcgggcgg cgcttccggg gttgcgaccg gatatgagtc
     2581 agcctgccgg gagtcttcgg gtgcgggcgg cacttcctgg atggcggcct gcttgttgat
     2641 cacaggaccg tcgaattctt tcgggatcgg atcatcgacc tgcgccgggg ccttctgctt
     2701 gcgcagttcc tcttcaatcc acctggcgat ttcttcgggg acttcgacgg gttcggggta
     2761 atccggatcc ggaaagtcct ccgcttccga cgcgggttcg gtttccagcg ccgggtccgc
     2821 ggccggggtg tcgtccagac cagtggtccc ttccggatcc gtgtcatcga ccagttccgg
     2881 aatgtcgtcc acggtcaggt tccgtgttga aacggcaaaa gaaacccgtt ggacacaagt
     2941 gtccttgttc atataggcac ctcctgtatt tttaatgcgt cgtctagaac cggaagccct
     3001 gaatcgacca tcgcggccct ggaagcgaat gaggcgactg tctcacaccc tgcggcatga
     3061 tttgggccgt tttcaaacaa atcctgcaaa atcctaattt tcatcgaccc agccatccgc
     3121 cgcttgattc gtctttcgcg cctctcctgc ctggccttgg tctcgggtac tttcatatgg
     3181 gacctccgga aaacagaaca aaccaatgtc cctactcaaa tccggcacaa actaagcggg
     3241 ttcggccagg gttttttcct ttcccgcctt tcagccgcct taatttgacg aatcgaaaag
     3301 ttcaaaaaag cacggaagga agaatcaacc tgccccgata agggcatcgg ccaggtccag
     3361 ggcgtcgcag ggccagaagg tgcgggggcg cgggcggcgg acgccggggc ggccggcttc
     3421 ggggcggcag gactgaaggg attgaagcca gcggccgggg accgccgatt cgccatgcac
     3481 ggcgccaaga agggcgccgc agatacaggc gttggtgtcg gtgtcgccgc cctgcatgat
     3541 cgtgtccacg atgccctctt ccagggacgt ggtatgcagc aactgccaca gggcgttctg
     3601 gaacgctatc agcacccaac cctgttggct ctggtagtcc tgcggcctgc gcgacgaggc
     3661 cgccttcacc acgccaacca ccgagtccac gtcacagtcc ccggcccatt caaggctctt
     3721 gccgtac
//
LOCUS       DAOPPA010000324         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26859_length_3724_cov_4.221859, whole genome shotgun sequence.
ACCESSION   DAOPPA010000324 DAOPPA010000000
VERSION     DAOPPA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26859_length_3724_cov_4.221859"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKH54_09915"
     CDS             <1..845
                     /locus_tag="PKH54_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00253.1"
                     /translation="GESWPIGTACGELAGGNACSSGYCFGQDVDLGRVGWCSRVCRDD
                     RDCPDLSYMGTGSFKWICGGLPILGGSSPSISDDLFASWCVPIDAESSLQDCADEREC
                     SSPGQTCLPLLRAGPPAPRDLVDYLCVAGTAGAALGSLCDPSGDGSDCASRLCMPAAA
                     AGVGFCSRTCRDIADCAVFGDGVDCDAVPIGRSRGGETLTADICRITGDCVVCRDDSD
                     CDSLHRCVNVASSWWYQDLRCVRTCAADSDCAASAGTSCSQIDWIMSASGDTFDACMT
                     PVCG"
     gene            872..1327
                     /gene="dtd"
                     /locus_tag="PKH54_09920"
     CDS             872..1327
                     /gene="dtd"
                     /locus_tag="PKH54_09920"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOD00254.1"
                     /translation="MKAVIQRVSSASVDVDGRVVGSIGSGLFVLVGLAAGDDASDIEY
                     IASKTAALRIFPDDAGRMNLCVAQAGGSILAVSQFTLIADTRKGNRPSFSAAMPVEDA
                     RLFWPRVVAGFTETGIPCEFGIFQGDMKCRLVNDGPVTIILDSAEGRCR"
     gene            complement(1436..2479)
                     /locus_tag="PKH54_09925"
     CDS             complement(1436..2479)
                     /locus_tag="PKH54_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00255.1"
                     /translation="MDQNESKMKHRDVEDLLLPDESGKNTIDIESELRKDSCDKPKKI
                     LTGSTITVIIVAVLAALSLIVIYSNDNLSEQFDAILKGELGKYKEELRKVQEDRIREI
                     EDLTGNKYGSVTLFYSPQDAQVMMKQMKYTKDCSSFAANEEDVLLCLKKPFDYTQKPE
                     VKEIDNKSLHLDKEKKEIVDSLPFNDMPIQEANEERTIVDAFEVEIEIIREGYDPRKF
                     HFTGDKNRIGVLGEGWESKYWDQKGPGIFMVDFQGADLNPKPETAKNNYMAAVKEMEC
                     IKREVAAKREAGKTVNQDTVNGVYSEILNKHEFKTVEEYNTVDNALRTTQPEWYEGFW
                     KEVEKMPCAAAGL"
     gene            complement(2643..>3724)
                     /locus_tag="PKH54_09930"
     CDS             complement(2643..>3724)
                     /locus_tag="PKH54_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00256.1"
                     /translation="VMPNQTLPSKAINLMDITGARVRRDGRTLVERDDIVHVLAAVSN
                     LPGDFLDMSLATREQNLKDRLKSRVFGCDSAIDAISSSVARNWARFGTRKPVGSFIFA
                     GAPGTGKSTLARELADAIFGNPDAFLLVPMADYSESHTLSNLIGAPAGFVGYEEGGLL
                     SDTLLRTPFLVIVWQNAHLADPSVLAQVVNIVRNGSITNRLGRRLDFRNSVQIITCES
                     PDACGGSSRGVGFGRDRCADDGTRIEAGIKKLLPPDLVREVDGTLAFQEPDEPTRRKV
                     TAKVLERALSEFLDANKVAIMATPEAIDTISELWNASRETGIDGVIASAVLKPAADMV
                     KLGRVPSGASMTVEPAGDGQVRLRL"
BASE COUNT          646 a         1070 c         1129 g          879 t
ORIGIN      
        1 cgggcgaatc gtggccgatc ggaaccgcct gcggggaact cgccggcgga aatgcgtgtt
       61 cgtccggata ctgctttggc caggacgtcg atttgggccg ggttggctgg tgttcccgcg
      121 tatgccgcga cgatcgggac tgtcccgatt tgtcgtacat gggtacggga tccttcaagt
      181 ggatttgtgg cggtcttccg atccttggcg gttcaagccc ttccatctcg gacgatcttt
      241 tcgcgtcgtg gtgtgttccc atcgacgcag aatcaagcct tcaggattgc gccgatgaac
      301 gcgagtgttc ttccccgggc cagacttgtc tgccactgct cagggccggc cctccggcgc
      361 cccgcgatct ggttgattat ctgtgcgttg ccgggacggc cggggcagcc ctgggctccc
      421 tctgcgatcc gtcgggcgac ggaagcgact gcgcatcacg attgtgcatg cctgcggccg
      481 ccgccggcgt cggcttctgc tcccggacgt gccgggacat cgcggattgc gcggtgttcg
      541 gcgacggcgt cgattgcgac gccgttccga ttggccgttc gcgcggcggc gagaccttga
      601 ccgccgatat ctgcaggatt actggcgact gcgtcgtctg tcgggacgat tccgactgcg
      661 acagccttca tcggtgcgtg aatgtggcct cgagctggtg gtatcaggat ttgcggtgtg
      721 tcaggacgtg tgccgccgac tcggactgtg ccgcctcggc cggaacgtcc tgcagccaga
      781 ttgactggat aatgtcggcg tccggcgaca cctttgacgc gtgcatgact ccagtgtgcg
      841 gttgacctgg ccgcggcttc ccgggggaga tatgaaggct gtaatccaga gggtatcctc
      901 ggcatcggtc gatgtcgatg gccgtgtcgt cggcagcatc ggatccgggc tgttcgtgct
      961 ggttggcctt gccgccggcg atgatgcttc tgacatcgaa tatatcgctt caaagactgc
     1021 tgccctgagg atctttcccg atgatgccgg ccggatgaac ctttgcgtcg cacaggccgg
     1081 cggttcaatt cttgccgtca gtcagttcac cctgattgcc gataccagga agggaaacag
     1141 gccgtccttc tcggcggcca tgcctgtcga agatgccagg ctgttctggc cgcgggttgt
     1201 cgcaggattc acggaaacag gaataccctg tgaattcggg atatttcagg gcgacatgaa
     1261 gtgccgcctg gtaaacgacg gtccggtgac aatcatcctt gattcggcag aaggtcgttg
     1321 tcgctaggtt gatccagccc gccacggaag atcaggtttc cgaagcggtt ccgaagtcga
     1381 acaacttcca aatcaatcaa attttcggat tcaatcggtt agatggattc agggatcaga
     1441 gaccggcggc ggcgcagggc atcttttcga cttccttcca gaagccttcg taccattcgg
     1501 gctgtgtcgt gcgcagggcg ttatcgacgg tgttgtattc ctcgacggtt ttgaactcgt
     1561 gcttgttcag gatttcgctg taaacgccgt tgacggtgtc ctggttaacg gtcttgccgg
     1621 cctcgcgctt ggcggcgact tcacgcttga tgcattccat ttccttgacg gccgccatgt
     1681 agttgttctt ggcggtttcg ggcttcgggt tcaggtcggc gccctggaaa tcgaccatga
     1741 aaatgccggg gcccttctga tcccagtact tcgattccca gccttcgcca agaacgccga
     1801 tacggttctt gtcgccggtg aagtggaact tgcgcgggtc gtaaccttcg cggatgattt
     1861 cgatctcgac ttcaaaagca tcaacgatcg tgcgttcctc gttggcttcc tggatcggca
     1921 tgtcgttgaa gggcagggag tcaacgattt ccttcttttc cttgtcaaga tgcagcgact
     1981 tgttgtcgat ctccttgacc tcgggcttct gcgtgtagtc gaacggcttc ttcaggcaca
     2041 ggagcacgtc ttcctcgttg gccgcgaagg aagagcagtc cttcgtgtat ttcatctgct
     2101 tcatcatgac ctgggcatcc tggggggagt agaacagcgt gacgctgccg tacttgttcc
     2161 cggtcagatc ctcgatttcg cggatgcggt cttcctgaac cttgcgaagt tcttctttgt
     2221 acttgcccag ttcgcccttc agaatcgcgt cgaactgctc ggaaagattg tcattgctgt
     2281 aaatgacaat aaggctgagg gcagccagca cggccacgat gatgacagtg atggtggaac
     2341 ctgtaaggat cttcttcggc ttgtcgcagg agtccttgcg gagttcgctt tcgatgtcga
     2401 ttgtgttctt tccgctttcg tccggcagaa ggagatcctc gacgtcccgg tgcttcatct
     2461 tgctttcgtt ctggtccatg ttacctcacc cgtccggtgc aaagacttgt tatgaatgca
     2521 ggggattaca ccctcgcgtg gcaatatatc ggtcacgtgc tgcccagtca aggtgaaatt
     2581 gttgccggaa cgcagtcttc gggaattggc gcaggtcgcc ccaatgggtc aggatcaggg
     2641 aatcagagac gcagacggac ttgcccgtca ccggcaggtt caacggtcat cgatgccccg
     2701 gacggaaccc ggccgagctt gaccatgtcc gccgccggct tcagcaccgc tgacgcgatc
     2761 acgccgtcga tgccggtttc cctggacgcg ttccacagtt ccgatatggt gtcgatggct
     2821 tccggggtcg ccatgattgc gaccttgttg gcgtcaagga attcggacag agcgcgctca
     2881 agcactttcg ccgtcacctt tcgccttgtc ggttcatccg gttcctggaa cgccagggta
     2941 ccgtcaactt cccttaccag atccggtgga agaagtttct tgatgccggc ttcaatcctg
     3001 gtgccgtcgt ccgcgcacct gtcccttccg aagccgaccc ccctcgagga accgccgcat
     3061 gcgtccgggg attcgcacgt aatgatctgc acggagtttc tgaaatcgag ccgtcggccg
     3121 aggcgattcg ttatcgagcc gtttcggact atgttgacca cctgggccag aaccgaggga
     3181 tccgccaggt gcgcattctg ccagacgatg accaggaatg gcgtgcgcag cagcgtgtcc
     3241 gacagcagcc cgccctcttc gtatccgaca aatccggccg gggcgccgat caggttcgaa
     3301 agggtgtgcg attcggagta atccgccatc gggacaagca ggaaggcgtc gggatttcca
     3361 aagattgcat cggcaagttc ccttgccagg gttgactttc cggtacccgg agcaccggcg
     3421 aatatgaacg atccgacagg cttgcgagtg ccgaatcttg cccagttgcg ggcaaccgac
     3481 gacgaaatcg cgtcgatcgc ggagtcgcac ccgaagaccc gtgacttcaa gcgatccttc
     3541 aagttctgtt cccttgtggc aagggacatg tccaggaagt ccccgggcag gttcgaaacc
     3601 gcggccagca catggacgat atcatcgcgc tcgacaaggg tgcgcccatc cctgcgcacg
     3661 cgcgcgccgg tgatgtccat caggttgatt gccttcgacg gcagtgtctg attcggcatc
     3721 acgc
//
LOCUS       DAOPPA010000325         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_26915_length_3718_cov_4.053235, whole genome shotgun sequence.
ACCESSION   DAOPPA010000325 DAOPPA010000000
VERSION     DAOPPA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26915_length_3718_cov_4.053235"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKH54_09935"
     CDS             <1..495
                     /locus_tag="PKH54_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00257.1"
                     /translation="TFCLLGDGECNEGVIWEALMAASHYRLTNLITIVDRNHLMIDGQ
                     TEDVMGLDPLDKKFEAFGFEVHVVDGHDLDQLAAAIERAMAVADADLAKTAGQRPHAE
                     IRAVGGKPVAIIADTVKGKGVDFMENKVEWHYGAVDSKLLADALASIDRMYDAIFARE
                     GVQP"
     gene            492..1508
                     /locus_tag="PKH54_09940"
     CDS             492..1508
                     /locus_tag="PKH54_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOD00258.1"
                     /translation="MSEITGTTWTCYDATTMTAREIYGRVLAELSDQDPRIVGLSADL
                     AKSTAISIFEKRHPERFFNVGIAEQNLMGVAAGMAKTGLIPFASTFAAFASMRACEQV
                     RTDICYQNLPVKIIATHGGMSFGAAGTTHHCTEDLAIMRSFANMTVIVPADGIETANA
                     VRAVKDLPGPAYIRIGRGFEPPFYETEDYGFQIGRAREIHPGTDLTIISCGIGVLQSF
                     EAARMLKESDGLSVRVLNMHTIKPIDREAILSAVMETRRILTVEEHNVLGGLGTAVAD
                     VIAESGKGCAFAKIGLPDSYAVMGYPEDLYAHYGLDANGIMEAVRKLLKVEFEEDEDW
                     NDEV"
     gene            1510..2313
                     /locus_tag="PKH54_09945"
     CDS             1510..2313
                     /locus_tag="PKH54_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00259.1"
                     /translation="MDQELMTARLFFRAAFPVMKVPLADDPAIAKAFKGVRAHVQFSA
                     DCDPALTPDGSGRVGACLHFDDGELTVVEELFETPDLVLHFPSLKAMNTLLRGGLAMP
                     RIRGGLKNFGLLVKMLTLLMRLTLMLPTNRPKDPLKRYLKVKMSIYMITTALSNFNRL
                     GNPRMREWTAKQPDRIYQFSVGGGEFGDFAAYLRVRGGQTKAGRGLYARRRPFVHFRF
                     TSVDGALAVLLKEVAFVEGVDKGCVSIDGSPEYAAQLNDLMAVLQGMLT"
     gene            2325..3110
                     /locus_tag="PKH54_09950"
     CDS             2325..3110
                     /locus_tag="PKH54_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00260.1"
                     /translation="MIDQSVKARVNLYGLFKALELLAQVDKEAAAAIAGRRLAIEFRV
                     RGVGRARLHLNDGQVRLQPPCGRESDGGRMEGGADIILWFKGPEHFNAMIDGRGQPIP
                     LKGFGHLKFLTGSFQVFMGRLAYYLLPTPELLQDPEFFAANTRMTAYVAFHALSEIAN
                     YDEEGMHSAHYIPDGILQVEVVDDIALYMVCQGGRLATYPGRHESPRCVLWFADIAAL
                     NDLLTGRVHTFDAVALGRMGMNGFVPMIDYLNPVLGRLPAYLK"
     gene            3142..>3718
                     /locus_tag="PKH54_09955"
     CDS             3142..>3718
                     /locus_tag="PKH54_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD00261.1"
                     /translation="MDGNSMKRQENTASRQMFERALKVIPTGIPGHLGPVNSQFIPVS
                     AYPFYVDHVQDSYFWDIDGNRYIDYMCAYGPNVLGYNNRVVEAAVQAQRARGDCMALP
                     SARQVELAELLVETITIADWAFFAKNGNDVTTLAAMTSRAATGRKKILMVKGGYHGAA
                     PWTQKAGRPGVTDEDVANNLCRMEQPEGVRTD"
BASE COUNT          741 a         1038 c         1202 g          737 t
ORIGIN      
        1 acgttctgcc ttcttggcga cggagaatgc aacgaaggcg tcatctggga ggccttgatg
       61 gccgcaagcc attaccgcct gaccaacctt atcaccattg tcgatcgcaa ccatctgatg
      121 atcgacggcc agactgaaga tgtaatgggg ttggatccgc tggacaagaa gttcgaggcg
      181 ttcgggttcg aggttcatgt ggttgacggg cacgacctgg atcagcttgc ggccgcgatt
      241 gaacgcgcca tggccgtggc cgacgcggac ctggcgaaga cggcgggcca gcggccgcac
      301 gccgagatac gggcggtcgg cggaaagccc gtcgcaatca tcgccgacac ggtcaagggc
      361 aagggcgtcg atttcatgga aaacaaggtc gaatggcatt acggcgcggt tgactcgaag
      421 ctcctggccg acgcgcttgc ttcgattgat cggatgtacg atgcaatttt cgcacgtgaa
      481 ggagtccagc catgagcgaa atcaccggaa ccacatggac ctgctacgac gcgacgacga
      541 tgacggcccg cgaaatctat gggcgcgttc tggccgagct ttccgaccag gatccgcgca
      601 tagtcggcct ttccgccgac ctggcgaagt caacggccat cagcattttc gagaagcgcc
      661 atcccgaaag gttcttcaac gtcggcatcg cggaacagaa cctgatgggc gtcgccgcgg
      721 gaatggccaa gaccggcctg attccattcg caagcacctt tgcggccttc gcgtcgatgc
      781 gtgcgtgcga gcaggtgcga accgatatct gttaccagaa cctgccggtg aagatcatcg
      841 cgacgcacgg cgggatgtct ttcggggccg ccgggacgac ccatcactgc accgaggacc
      901 tggccatcat gcgcagcttc gcgaacatga ccgtgatcgt gcctgccgac gggatcgaaa
      961 ccgccaacgc ggtccgggcc gtcaaggatc ttcccggtcc cgcctatatc cgcatcggcc
     1021 gcggtttcga gccgccgttc tatgaaaccg aggattacgg cttccagatc ggccgggcca
     1081 gggagattca tcccggcacc gacctgacca tcatttcgtg cggaatcggc gtgcttcagt
     1141 ccttcgaggc ggcgcgcatg ctgaaggagt cggacggtct ttccgtccgc gtcctgaaca
     1201 tgcacacaat caagccgatc gatcgtgaag caatactttc ggcggtcatg gagaccaggc
     1261 gcatcctgac tgtcgaggag cataacgtcc ttggcgggct tggaacggct gttgcggacg
     1321 tgatcgctga atcgggcaag ggatgcgcct tcgcgaagat cggccttccg gactcgtatg
     1381 ccgtcatggg atatcccgag gatctttacg cgcattacgg gcttgacgcc aatggcatca
     1441 tggaagcggt gcgcaagctg ctgaaggtgg agttcgagga agacgaagac tggaatgatg
     1501 aggtctgaca tggaccagga attgatgacg gcgcgtcttt tcttcagggc cgcgtttccg
     1561 gtcatgaagg tgccgctggc ggacgacccg gcgattgcaa aggctttcaa gggcgtgcgc
     1621 gcgcacgtcc agttttcggc cgactgcgat ccggcactga cgcccgacgg aagcggtcgg
     1681 gtgggggcct gcctgcattt tgacgacggt gaattgaccg ttgtcgagga acttttcgaa
     1741 accccggatc ttgtcctgca ttttccaagc ctgaaggcga tgaacacgct tcttcgcggt
     1801 ggccttgcaa tgccgcggat ccgcggcggc ctgaagaatt tcgggcttct ggtcaagatg
     1861 ctgacactgc tgatgcgcct gacgctgatg ctgccgacga accgtccaaa ggatccgttg
     1921 aagcgctatc tgaaggtgaa aatgtcgatc tacatgatca ccacggcgct ttccaacttc
     1981 aaccgactgg gcaacccgcg gatgcgtgaa tggaccgcga agcagcccga ccggatttat
     2041 cagttcagcg tcggcggcgg ggagttcggg gatttcgcgg cgtatctgag ggtgcgcggg
     2101 ggccagacga aggcggggcg cgggctttac gccaggcggc gtccgttcgt gcatttcagg
     2161 ttcaccagcg ttgacggggc cctggccgtg ctgctgaagg aagttgcgtt cgtcgagggc
     2221 gtcgacaagg gttgcgtgtc gattgacggc agcccggagt acgccgcgca gcttaacgac
     2281 ctgatggctg tcctgcaggg aatgttgacc tgacggaggg tgcgatgatt gatcagtcgg
     2341 ttaaggcaag ggtgaacctg tacgggctgt tcaaggcgct tgagctgctt gcgcaggttg
     2401 ataaagaggc cgcggcggcg attgccggac gccggcttgc gattgaattc cgtgtgcgcg
     2461 gtgtcggtcg cgccaggctt cacctgaatg acggtcaggt gcgacttcag ccgccgtgcg
     2521 gccgcgaatc ggatggcggc cgcatggaag gcggggccga catcatcctg tggttcaagg
     2581 ggccggaaca tttcaacgcg atgatcgacg gtcgcggtca gccgattccg ttgaagggat
     2641 tcggtcatct gaagttcctg accggttcgt tccaggtctt catgggacgg cttgcgtatt
     2701 acctgctgcc gactcccgaa ctgcttcagg atcctgaatt ttttgccgcc aacacacgca
     2761 tgaccgccta cgtggcgttc catgcgcttt ccgaaatcgc caattacgac gaagaaggga
     2821 tgcacagcgc gcattacata ccggacggaa tcctgcaggt ggaagtcgtc gatgacatcg
     2881 cgctttacat ggtctgccag ggagggcgtc ttgcaacgta tcccggcagg cacgagtcgc
     2941 cgcgttgcgt cctgtggttt gccgatatcg ccgccttgaa cgatttgctg acgggcaggg
     3001 tgcacacctt tgacgccgtc gcgctggggc gcatgggcat gaacggtttc gtgccgatga
     3061 ttgattacct gaatcccgtg ctgggcaggc ttccggcata cctgaaatag tcgccattgg
     3121 gcaccgcatg gagaacgcac catggatgga aattcgatga agcgccagga aaacacggca
     3181 agccgtcaga tgttcgaaag ggctctgaag gtgattccga ccgggatccc gggccatctg
     3241 ggcccggtca attcgcagtt cataccggtc agtgcttacc cgttttacgt cgatcacgtc
     3301 caggactcgt atttctggga catcgatggg aacaggtata tcgattacat gtgcgcctat
     3361 ggaccgaacg tcctcggata caacaaccgc gtggtcgagg cggccgttca ggcccagcgt
     3421 gccaggggcg actgcatggc gcttccgtcg gccaggcagg tcgaactggc cgagctgctg
     3481 gttgaaacga taacgattgc cgactgggca ttcttcgcga agaacggcaa cgacgtcacc
     3541 acgctggccg ccatgacttc gcgcgcggcg accgggcgca agaagatcct gatggtcaag
     3601 ggcggttatc acggggcggc gccgtggacc cagaaggcgg gaaggccggg cgtcaccgac
     3661 gaggatgtcg cgaataatct atgtcgaatg gaacaacctg aaggagttcg aacagata
//
LOCUS       DAOPPA010000326         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27039_length_3706_cov_3.493837, whole genome shotgun sequence.
ACCESSION   DAOPPA010000326 DAOPPA010000000
VERSION     DAOPPA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27039_length_3706_cov_3.493837"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PKH54_09960"
     CDS             complement(<1..1848)
                     /locus_tag="PKH54_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00262.1"
                     /translation="MKKTLIPVLAVAAALLMSGCSQEYLPMLDEGGQSDIVADYQVPD
                     DVVNFDEGRDLATDDGQQHRYQILLQVDPETPVQATVGNQIQVSAIVVDYIQGQPAPD
                     VLVDFQIVTVVDDDGVVVQGDANFETYSRYTDPKGRVSNDFFVGMVPEVTYTLGLSLP
                     DYPESEPVTMKVKVLAYACGCLRVDFSYDGVIETPLRDIEVFVVPSTYQCGKHIFPHT
                     VLSDDLIIAQRSAQTVNSVVTFDCLPAGATYTVFARAGSGDLVCTAAGGCSRTLVMQP
                     ETCDEMTLEFSEVTFQASGRYNAVDHFNFEPMIEMCAGGDTTIIGCITAGTEDVGKTV
                     CCAIQELVKLFNTPGSTIVQLVFDALKLWIGSAIIDAVQTVVGKALESVLNDLILQWS
                     RDTPWLAAFLTAGKDITQIISNLELESELVLQKLGSDYTVQGTHFWHAINLYWRMGCA
                     AEGDPAFDPECGKYTLDMKDLTVLPDDFPMELLEGSFIADVLNFNWMSLRQHEIALSY
                     GKLVLWAINGIFIPQISGDQAHSIDDLARLWLDCKGMANGPIGDAAETIPGVDRDDVE
                     AVCNTVITSLIDPVDDMIGRLSLSTTLTLTGTGRMLDGNCDTIIDSIVDG"
     gene            2135..>3706
                     /locus_tag="PKH54_09965"
     CDS             2135..>3706
                     /locus_tag="PKH54_09965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD00263.1"
                     /translation="MSQNDDGIGRAGLGAGVSGPVQDGSGEEAATTVFSDLFCPICGG
                     RFPGGLSVCTEDGVALVPVGQGDDLSGTVLNNKYVLVQLIGRGGFGNVYRATHKLAKT
                     ELAIKVIKDDLRDNEKAVRQFLKETRALMSIQSMHVVTVHDVDRDTAGRIFIVMELLR
                     GRDLEDHVRLEYSSTGRMPWAAVGRIVLEVCDALECAHEKGIVHRDLKPGNVMMVKGR
                     GGKLMAKVVDFGIASLAQSENMDLTTMDAVPRVIGTPSFMSPEQAKGQPVDSRADLYS
                     LGAMVYRLVCGCKPFDAKTDQGMLIAHVIEKPRPIRERCPELEVPQEFDALIMQLMAK
                     TPEERPARVEDVAARLRAILPEEDRQPMRGQGIAGWKIAVAVAAVLLVAAGAAAFFLL
                     SGKETPAIATAADATVADTAVAAQPAAPVEPVQAAPVVEPVAPGASSGVAVSPTPAPQ
                     PAAAQIPVAPAPAAQPAVAAEVPAAAPAQPAPAPAATPAGPAPRAQLPAVPVPTATST
                     ASGGNIVAPSVAGGGA"
BASE COUNT          700 a         1106 c         1142 g          758 t
ORIGIN      
        1 cccatcgaca atgctgtcga tgatcgtgtc acagtttccg tccagcatcc gtccggtgcc
       61 ggtcagtgtc agagtcgtgg aaagagagag ccttccaatc atgtcgtcaa ccgggtcgat
      121 gagtgaggtg attaccgtgt tgcagacggc ctcgacatcg tcgcggtcaa cacccggtat
      181 cgtctcggcg gcgtcgccga tcgggccgtt tgccattccc ttgcaatcca gccagagcct
      241 ggcgaggtcg tcgattgaat gtgcctggtc gccggaaatc tgcgggatga aaatgccgtt
      301 tatcgcccac agcaccagct ttccgtagct caaggcgatt tcgtgctgcc gcagcgacat
      361 ccagttgaag ttcaggacgt ccgcgatgaa gctgccctca agcagttcca tcgggaagtc
      421 gtcgggaagc accgtcagat ccttcatgtc cagcgtgtat ttgccgcatt cgggatcaaa
      481 ggccgggtcg ccttccgctg cgcaccccat cctccagtac aggttgatcg cgtgccagaa
      541 atgggttccc tgaacggtgt agtcgctgcc gagtttctgc aggacaagct ccgactccag
      601 ttccagatta ctgatgatct gggtgatgtc ctttcccgcg gtcaggaacg ccgccagcca
      661 cggtgtgtct cttgaccact gtagaatcag gtcgttgaga acggattcaa gagctttgcc
      721 gaccacggtc tggacagcat cgataatggc gcttccgatc cagagtttca gcgcgtcgaa
      781 aacgagctgc acgattgtcg agccaggggt gttgaacagc tttaccagtt cctggatcgc
      841 gcagcatacc gtcttgccca cgtcctcggt gccggccgtg atgcatccga taatcgtggt
      901 gtccccgccg gcgcacatct cgatcatcgg ttcgaagttg aagtggtcaa ccgcgttgta
      961 acggccgctg gcctggaatg ttacttccga aaattccagg gtcatttcgt cgcacgtttc
     1021 cggctgcatg accagcgtcc tggagcatcc accggcggcg gtgcagacca ggtcgccgct
     1081 gccggccctt gcgaagaccg tgtaggtcgc gccggcaggc aggcagtcga aggtgacgac
     1141 cgagttgacc gtctgggcgc ttcgctgggc gatgatcagg tcgtcggaga gcaccgtgtg
     1201 cggaaaaatg tgctttccgc actggtaggt cgaaggaacg acgaatacct cgatatcgcg
     1261 caggggcgtc tcgatcacgc catcgtacga aaaatcgacg cgaaggcatc cgcaggcgta
     1321 cgcaagaacc ttcaccttca tggtgaccgg ttccgattcg ggatagtcgg gaagcgaaag
     1381 ccccagcgtg taagtgactt ccggaaccat cccgacaaaa aagtcgttcg acacgcggcc
     1441 tttggggtcg gtgtacctgc tgtacgtttc aaagttcgcg tcaccctgga ccacgacgcc
     1501 gtcatcgtca acaacggtca caatctggaa gtcaaccagc acgtccgggg cgggctgtcc
     1561 ctgaatgtaa tcgaccacga tcgccgaaac ctggatctgg tttccgacag tggcctgaac
     1621 cggggtctcg ggatcgacct ggagcaggat ctggtacctg tgctgctgac cgtcgtcggt
     1681 cgccagatcc cttccctcat cgaagttcac gacgtcatcc ggaacctggt agtcggccac
     1741 gatgtccgac tgcccgccct cgtccagcat cggaaggtat tcctgtgagc acccggacat
     1801 cagtaaggca gcggcaaccg ccagaaccgg aatcaaggtc tttttcatat tcttacctta
     1861 acctgtcaat cggcggcgtg agtcgccagg caggaaaaat gacgcgggta ttatagtcta
     1921 tcggcggcat gcaggccaga tggcccgcgc atttccacga aactgtccat aaaggcagga
     1981 ggatgatcga caagtcgtcc cctggatcag ccaacgatgt ctttattccg gtttgacaag
     2041 gtggtatcat cccgcgggta agcgatggtt cagtcccgtt cggtccatca aatcggggac
     2101 tgatgttgtt ttttaattga agtcagggtg tttcatgagt cagaacgatg acggaatcgg
     2161 gcgtgcgggg ctgggggctg gcgtttccgg gcctgttcag gacggaagtg gtgaggaagc
     2221 ggcgaccacg gttttttcgg atctgttctg cccaatctgt ggcggccggt ttcccggtgg
     2281 cctttccgtc tgcaccgagg acggggttgc ccttgttccg gtcggacagg gcgacgacct
     2341 ttcgggaacc gttctgaaca acaagtacgt gcttgtccag ctcatcggtc gcggtgggtt
     2401 cggaaacgtc tatcgggcga cccacaagct ggcaaagacc gaactcgcca tcaaggtcat
     2461 caaggacgat cttcgtgaca atgaaaaggc cgtgcggcag ttcctgaagg aaacacgcgc
     2521 actgatgtcg atacagtcga tgcatgtcgt gaccgttcac gacgtcgatc gcgacacggc
     2581 gggacgaata ttcatcgtca tggaacttct gcgcggccgc gatctggaag accacgtcag
     2641 gctggagtat tcgtcaaccg ggcgcatgcc gtgggccgcg gtcggccgca tcgtcctgga
     2701 agtgtgcgac gcgctggaat gcgcccatga aaagggaatc gtccatcgcg acctgaagcc
     2761 cggaaacgtc atgatggtga aggggcgtgg cgggaagttg atggcgaagg tcgtcgattt
     2821 cggcatcgcc agcctggctc agtccgaaaa catggatctg actaccatgg acgccgttcc
     2881 aagggtcatc ggaacgccat ctttcatgag tcccgagcag gcgaagggtc agccggtcga
     2941 ttccagggcc gacctttaca gccttggcgc catggtttat cggctggttt gcggttgcaa
     3001 gccgtttgat gccaagaccg atcagggcat gctgattgcc catgtcattg aaaagccccg
     3061 gccgatccgc gaaaggtgtc cggaacttga ggttccccag gagttcgatg cgctgatcat
     3121 gcagttgatg gcgaagactc ccgaggaacg tccggcccgt gtcgaggacg ttgcggcccg
     3181 cctgcgcgcg attctgcccg aggaagaccg ccagccgatg cgcggtcagg ggatcgccgg
     3241 ctggaagatc gctgtcgccg ttgcggccgt gcttcttgtt gccgccggcg ccgccgcatt
     3301 tttccttctt tccggcaagg aaacgccggc gattgcgacc gctgcggacg ccaccgttgc
     3361 cgatacggca gttgccgcgc agccggcggc tcccgtcgag ccagtccagg ccgctccggt
     3421 tgttgaaccc gtcgcgcccg gcgcttcttc cggtgtcgcc gtttcgccga cgccggctcc
     3481 gcagcctgcc gcggcgcaga ttccggttgc tccggccccg gccgcacagc cggcagttgc
     3541 cgccgaagtt cccgcggccg caccggcgca gccggcccca gcccccgcgg cgacccctgc
     3601 cgggccggcg cccagggccc agcttccggc cgttcccgtt ccaacggcga catccacggc
     3661 ctcgggcggg aacattgtcg cgccatcggt cgcgggcggt ggagcg
//
LOCUS       DAOPPA010000327         3689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27203_length_3689_cov_4.596037, whole genome shotgun sequence.
ACCESSION   DAOPPA010000327 DAOPPA010000000
VERSION     DAOPPA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27203_length_3689_cov_4.596037"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="PKH54_09970"
     CDS             <1..1613
                     /locus_tag="PKH54_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00264.1"
                     /translation="NPMQKFPRRILGILVTGLLLAGAGCPGEPQEVVMPDLPEWFPDK
                     PQSPDFALQRKDGGTFPTPEEVDGFTETYSSFLKRTDFIGWIDRHAQGLAPDNPWGMS
                     DYKIWWAQAFMTRSGDLVTLAFRGPPDNTTQSVGRVLGATAGLYMQSEDETARNLSMG
                     LVRGLSATFDGMIWGDEDPVIDTIMARTIFNRSHETMLRGNRRLFVDYEGVRFEEVEL
                     RHDTIHNRDNPTWGDIFVRNKRSKDDFPFLYRNMAMLVELAFRTDDPEVRRDIVRLVG
                     QVRAMTADIVEHDYCIRTKGFDSKPYIPLMQDGTVEDFATYRAYESFVPLGECTGKLS
                     TAYLASGEALGNVCENADTAFYGDGYLYEELAIAAHFCATDLIWNYHVAALAMALLFD
                     DIETARKLVEGLAARIDGLWIDERAADYPEWWPEVAQLLVLSAAYGMPLTGDEARLIQ
                     SVWRDSAVFYQDSVCWDPWDPSVPDGTVCEVMPSRYETDDQGQTTRAHTRIEEFLSPF
                     EYCASPYRATTGAMFVNCERLVAAMMDR"
     gene            1656..2147
                     /locus_tag="PKH54_09975"
     CDS             1656..2147
                     /locus_tag="PKH54_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD00265.1"
                     /translation="MDRDSKRTEVTERILADTRSGREPQAIVQAAMRRIKDSFPEFYW
                     VGVYLLFGDELRLGPFCGPVTEHVRIPVGRGVCGTAVAENRNQVIGDVRAVSNYLACN
                     LFTRSEIVVLIRDPDDKSVVGQIDIDCTVPDRFGSDDEAFLDEIGRLLAPAIVAMRRH
                     DRG"
     gene            2152..3141
                     /locus_tag="PKH54_09980"
     CDS             2152..3141
                     /locus_tag="PKH54_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD00266.1"
                     /translation="MGLPEHVSSVAPGVWRIRVPLPGSPLRNLNSWVVTSDGQALVID
                     TGFNIPECLEAMKSGLAAIGANPSRTGFMLTHMHSDHTGLVGRLAAPGTDIFMGRLDA
                     PFMDPGMNWWNPMVDYAVQNGFGRQQISDALASHPGIKFHPGAIGPMKLLEDGDSITV
                     GDRELACVLTPGHTPGHICLYDRQARLLFSGDHVLGDITPHIESWAYRFNALKCFMSS
                     LEKIRGLDVVQALPGHRGTIAELGPRIDQLVAHHAARADETVQVLGGGCMTAYEIASR
                     MTWDVRAPSWEQFPITQKWFAVGETIAHLRWLEDSGRVRRQIEDGIARFCVGE"
     gene            complement(3145..>3689)
                     /locus_tag="PKH54_09985"
     CDS             complement(3145..>3689)
                     /locus_tag="PKH54_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD00267.1"
                     /translation="LLADLMDMGSLGAVGDDDMKAVDIGSVLDRVCDMLRLDADNKRI
                     SLKVVVSARRSLRARENHIERLVENLAGNAIKYTPEGGRVEVGLAAGRAGLVLSVADS
                     GVGISADSLPHVFEEFYRADNVRKSHEGTGLGLALVKRIVDRYGASIRVSSNPGAGTT
                     FVVTWPDAALEPEHGAEPVE"
BASE COUNT          753 a         1109 c         1117 g          710 t
ORIGIN      
        1 gaaatcccat gcagaaattt ccccgcagaa ttctcggaat tcttgttacc ggcctgctgc
       61 ttgcaggcgc tggatgcccc ggcgaacccc aggaagttgt gatgccggat ctgccggagt
      121 ggtttccaga caagccacaa agccccgact ttgccctgca gcggaaggat ggcggaactt
      181 tcccgactcc ggaggaagtt gacggattca cagaaaccta ctcgtccttc ctcaagcgga
      241 ccgacttcat cggctggatc gaccgtcatg ctcagggcct ggcgccggac aatccctggg
      301 ggatgtctga ctacaagatc tggtgggccc aggcattcat gaccagaagc ggcgatctgg
      361 tcacccttgc tttcagggga ccgcccgaca acacgacaca gagcgtgggc cgggtacttg
      421 gcgccacggc cggtctgtac atgcagtccg aagacgagac cgcgcggaat ctctcaatgg
      481 ggcttgtccg tggactgtcc gccacctttg acggaatgat ctggggcgac gaggatccgg
      541 tcattgacac gataatggcc aggacaatct tcaaccgaag tcacgaaacc atgctgcggg
      601 gcaacagacg cctgttcgtg gactacgaag gcgtccgttt cgaggaagtc gaactcaggc
      661 acgacacaat ccacaaccgg gacaatccca cctggggcga catattcgtc cggaacaaga
      721 gatcaaagga cgattttccc tttctttatc gaaacatggc catgctggtc gaactggcct
      781 tcaggacgga cgatcccgaa gtcaggcgcg acattgtcag gctggtcgga caggtccgcg
      841 ccatgacggc cgacatcgtc gaacacgact actgcatccg gacaaagggc ttcgattcga
      901 agccctacat cccgttgatg caggatggaa cggtcgagga ctttgccacc taccgggctt
      961 acgaatcgtt cgtgcccctg ggcgagtgca cgggaaaact ttcgaccgca tacctggcat
     1021 ccggcgaggc attgggaaac gtctgtgaaa acgccgacac cgccttttac ggcgacggat
     1081 acctgtatga ggaactcgcg attgcggcgc atttctgcgc gaccgacctg atctggaact
     1141 accatgtggc ggcgcttgcg atggcgctgc tttttgacga catcgaaacc gccaggaaac
     1201 tcgtcgaagg acttgcggcc aggattgacg gcctgtggat cgacgaaagg gcggccgatt
     1261 acccggaatg gtggcccgag gtggcccagc tcctggtgct ctcggccgcc tacggaatgc
     1321 ccctgaccgg ggacgaagca aggctgattc agtccgtatg gcgcgactcg gccgtattct
     1381 atcaggactc ggtatgctgg gatccctggg atccatccgt tccagacggc accgtctgcg
     1441 aggtgatgcc atcacgctat gaaacggacg accagggcca gacgacgcgc gcgcacacca
     1501 ggatcgagga gttcctgagc ccgttcgagt attgcgcgtc gccataccgg gcgaccaccg
     1561 gcgcgatgtt cgtgaactgt gaaaggcttg ttgcggccat gatggatcgc tgattatgcc
     1621 ggttacttcc aaaacggatc ggcgggggtg attaaatgga ccgcgatagc aaacgcactg
     1681 aggttaccga gcgaatcctg gcggacaccc gcagtggccg cgaaccccag gcaatcgttc
     1741 aggcggccat gcgccgcatc aaggactcat ttccggaatt ttactgggtt ggcgtgtatc
     1801 tgctgttcgg cgacgaactg cgccttggcc ccttctgcgg cccggtgacg gaacacgtcc
     1861 ggatcccggt cggacgcggc gtctgcggga ccgcggtggc agaaaaccgg aaccaggtca
     1921 tcggggacgt gcgggcggtg tcgaattacc tggcctgcaa ccttttcacg agatcggaaa
     1981 tcgtggtgct tatccgggat ccggatgaca aatcggtggt cggccagatc gacatcgact
     2041 gtaccgttcc ggatcggttt ggatcggatg acgaggcatt cctggacgaa atcggccggc
     2101 tcctcgcgcc ggcaattgtc gcaatgcgtc ggcatgaccg ggggtgatca agtggggctt
     2161 cccgaacacg tctcttccgt ggcgcccggg gtctggcgca tcagggttcc gctgcccggc
     2221 agcccgctca ggaacctgaa ttcgtgggtc gtgacgtcgg acggccaggc gcttgtgatc
     2281 gataccggat tcaacatacc ggagtgtctg gaggcgatga agtccggcct ggccgccatc
     2341 ggggcgaatc cttcccgtac cggtttcatg cttacccaca tgcattcgga tcataccggc
     2401 ctggtcggcc gcctggcggc accggggact gacatcttca tgggtcgtct ggatgcccct
     2461 ttcatggatc ccggaatgaa ctggtggaat cccatggtcg attacgccgt tcaaaacggc
     2521 ttcggaaggc agcagatttc cgacgcgctt gccagccatc ccggaatcaa gttccacccg
     2581 ggcgcgatcg ggccgatgaa acttctggaa gacggcgatt ccatcaccgt tggcgacagg
     2641 gaactcgcgt gcgtgctgac acccggacat acgcccggcc acatctgcct ttacgaccgg
     2701 caggcccggc tgctgttttc cggagatcac gttcttgggg atatcactcc gcacatagag
     2761 tcctgggcgt accgcttcaa cgccctgaag tgcttcatgt ccagtcttga aaagatccgc
     2821 ggcctcgacg tggtgcaggc ccttcccgga catcgaggaa caatcgcgga actcgggccc
     2881 agaatcgacc agctcgtcgc ccatcatgca gccagggcgg acgaaacggt tcaagtgctg
     2941 ggcgggggct gcatgactgc ttacgaaatc gcttcacgca tgacatggga cgtcagggcc
     3001 ccttcctggg aacaattccc gatcacccag aaatggttcg cggttggcga gacgattgca
     3061 cacctgcgct ggcttgagga ttctggaaga gtccgccgcc agatcgagga tgggatcgca
     3121 agattctgcg tgggagagtg aaggtcactc gaccggttcc gcgccgtgtt caggttcaag
     3181 ggcagcgtca ggccaggtta cgacgaaagt ggttcccgcg cccgggttgc tggatacccg
     3241 aatcgaggca ccatagcggt caacgatcct cttgaccagc gcaagaccaa ggccagtgcc
     3301 ttcatgcgac ttcctgacat tgtccgcgcg atagaactcc tcgaacacgt gcggcagcga
     3361 atcggctgat attcccacgc cggaatccgc cacgctcagg acgaggcctg ccctgccggc
     3421 ggcaagaccg acttccaccc gaccaccttc gggggtgtat ttgatcgcgt tgcctgcaag
     3481 attctcgaca aggcgctcaa tgtggttctc cctggcccga agcgatcgcc gggccgaaac
     3541 cacgactttc agcgatatcc gcttgttgtc ggcatccagg cgcagcatgt cgcagactcg
     3601 atccagcacc gagccgatat cgacagcctt catatcgtcg tcgccgaccg ccccgagcga
     3661 acccatgtcc atcaggtcgg caagcaacg
//
LOCUS       DAOPPA010000328         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27249_length_3684_cov_3.664646, whole genome shotgun sequence.
ACCESSION   DAOPPA010000328 DAOPPA010000000
VERSION     DAOPPA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27249_length_3684_cov_3.664646"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKH54_09990"
     CDS             complement(<1..491)
                     /locus_tag="PKH54_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD00268.1"
                     /translation="MNEPTIDTTEMTFETMGLPPWLLDGVRAMGFERPMEVQARCFGP
                     AMDGRDLVVQSRTGSGKTAAFTLPLLAQVKPTGIPTVLALGPTRELALQVHGVIEQLG
                     KPGGLRSACIYGGTGFTAQLEALHDGVDIVVGTPGRILDHARRGTLKLSGITRVVLDE
                     ADE"
     gene            697..942
                     /gene="xseB"
                     /locus_tag="PKH54_09995"
     CDS             697..942
                     /gene="xseB"
                     /locus_tag="PKH54_09995"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOD00269.1"
                     /translation="METTEKTFEQLLGQIDDILQKLEQDDLDLEQSLKLYEDGMSAYR
                     DANAKLAAARGRLAQLVAIDENGNQTTVPISLNNVED"
     gene            993..2420
                     /locus_tag="PKH54_10000"
     CDS             993..2420
                     /locus_tag="PKH54_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOD00270.1"
                     /translation="MKTSNNQSRFVSLMISGTVVLCCLLLAGGPADGKPVGVIAMQAG
                     GYANHDSGGERIVPLLVFPISTVGMTLSSPELSAVREILSVNLARGGRFSIMAESDIR
                     KRLSEQKVTSWDDCFDDLCRIELGAAIHADVGVVSKILKVSDECSLSVTMYDFRTELT
                     IDASTVDFDCSVKGLKAAVISVAATLRGDAPQPVSQIASQPAAVAGNAPPVVVNPNQA
                     RGPRDDGTAVRPSRSGKQPSAPSDPAGHSWVYVPGGTFRMGDPDGQADERPVHDVTLP
                     PFFVTRTEVTVEQYSRCVQAGRCSEPGVEKGCTWVNRKAGMPVNCVTWAQAEQFAAWA
                     GGRLPAESEWEFVARGGSGGFAWASATVAPQCPGAIIRGVTPGCGQNGPALVCSLRAD
                     SPTGRPCDLDGNLREWVRDWYSARYEPGSTAPGYVGPKRGSLRSVRGAGWLSFQAATR
                     SAARDAENPDRFFIDVGFRVVVPAQ"
     gene            complement(2457..2639)
                     /locus_tag="PKH54_10005"
     CDS             complement(2457..2639)
                     /locus_tag="PKH54_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00271.1"
                     /translation="MMKRFATILALSILCLTALAALGGCQEQQVRRYPFCLSDGDCNK
                     GQICVSQLCTSPTRTQ"
     gene            complement(2661..3512)
                     /locus_tag="PKH54_10010"
     CDS             complement(2661..3512)
                     /locus_tag="PKH54_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00272.1"
                     /translation="MMIKRTAPTKPVVHQVSGLSRVPAKVLLLTSDETKRAEPQVQQQ
                     PQYQPDYYAQGAQQPAFNILQLWGMARRGPALPEASALLDKDSAANNALVSAVEHSLM
                     AKGFLLVDKGVVSRIEQYLGANNHNTRESWNTLEKALLLGKETGADAIVQVRTMKVFK
                     ETKIYALRNGENVFNPVPDKYGYNLMWSGQGSILPREWWSVMVEIRMINIQGEVIWAG
                     FKEVKLTDVLPEDWKAVLLPQFGGYAMVSEANIPYETYANDEALIRNKLTDALFEMLN
                     MIPSPTN"
BASE COUNT          704 a         1102 c         1084 g          794 t
ORIGIN      
        1 atttcgtcgg cctcgtcaag cacgaccctg gtgattcccg aaagcttcag ggttccccgc
       61 ctggcatgat ccagtatgcg gccaggcgtg ccgaccacaa tatcgacgcc gtcatgaagc
      121 gcctcaagct gggccgtgaa accggtcccg ccgtaaatgc aggcgctgcg aagaccgccc
      181 ggcttgccga gctgctcgat cactccatgc acttgaaggg caagttccct tgtcgggcca
      241 agggccagaa cggtcgggat cccggttggc ttcacctgcg ccagaagggg cagggtgaaa
      301 gccgcggtct tgccggaacc ggttcgtgac tgcaccacca ggtcgcgtcc gtccatggcg
      361 gggccgaagc agcgggcctg aacctccatc gggcgttcga aacccatggc gcgaaccccg
      421 tcaagcagcc agggcggcaa tcccatggtc tcgaaagtca tttctgtcgt gtcgattgtc
      481 ggttcgttca attcagtcgt atcgattgcg ttattcctgt gtggcggatt gtgtccgcgc
      541 gtcccgcttc gtgtcgcggc caggtcccgc acggtggatc gaatccgtcg gttcgcgacc
      601 tgccgcgtca aggtacaaaa aatatcacgt gttgtaaagt ggcacgcccg cccgcggtga
      661 acgataatta ctgcgtctga tggaattggt gaccgaatgg aaacaaccga aaagaccttt
      721 gaacagttgc ttggccagat tgacgacatc cttcagaagc tggaacagga cgaccttgat
      781 ctcgagcaat ccctgaagct ttacgaagac ggcatgagcg cgtaccggga tgccaatgca
      841 aaactggccg ccgccagggg ccgtcttgcc cagcttgtgg cgatcgacga aaacggcaac
      901 cagacgaccg tcccaatctc cttgaataac gtggaagatt gatccgcctt tccgccttta
      961 tttccggctt ggtgtctatt aacatcgcgt ggatgaagac cagcaacaat cagtcccggt
     1021 tcgttagtct gatgatttcc ggcacggtcg tactgtgctg cctgctgctt gccggtggcc
     1081 cggcagacgg caagccggtg ggcgtcatcg cgatgcaggc cggcggttac gcaaatcacg
     1141 attcgggtgg cgagcgcatc gtccccttgc ttgtgtttcc catctcgacg gtcgggatga
     1201 ccctttcgtc gccggagttg tctgccgttc gtgaaatcct ttcggtgaac cttgcgcgag
     1261 gcggccgctt ttccatcatg gccgagtccg acatccgtaa acggctttcg gaacagaagg
     1321 ttacgtcctg ggacgattgc tttgacgatc tgtgccgtat cgaacttggc gcggcaatcc
     1381 atgccgatgt cggggttgta tccaagatcc tgaaggtttc cgatgaatgt tcattaagcg
     1441 tgaccatgta cgattttcga accgagctga cgatcgacgc atctacggtc gatttcgact
     1501 gttcggtcaa ggggctgaag gccgcggtca tttccgttgc ggcaaccctg cgcggcgacg
     1561 cgccgcagcc ggtttcccag atcgcttccc agcctgccgc cgtggctggc aacgcaccgc
     1621 cagtcgttgt taaccccaac caggcccgag gcccccggga tgacggaacg gccgtccgcc
     1681 cctccaggtc gggaaagcag ccttcggccc catcggatcc ggccggccat tcctgggttt
     1741 atgtcccggg cggcacattc cgcatgggcg atcccgacgg acaggccgac gaacgcccgg
     1801 ttcatgacgt gaccctgccg cctttctttg taacccgaac cgaagtcact gttgagcagt
     1861 actcccgttg cgtccaggcc ggccgctgtt ccgagcccgg ggtcgaaaag ggatgcacat
     1921 gggtgaatcg caaggccggg atgcccgtga attgtgttac ctgggcacag gccgagcagt
     1981 tcgcggcctg ggcgggtggc cggcttccag ccgagtcgga atgggagttc gttgcgcgtg
     2041 gtggctccgg cggctttgcc tgggccagcg ccaccgtcgc gccgcagtgt cccggcgcga
     2101 tcatccgtgg cgtcaccccg ggctgtggtc agaacggccc ggccctggtc tgttcacttc
     2161 gcgccgattc cccgaccggc cggccgtgtg acctggatgg aaacctgcgg gaatgggttc
     2221 gcgactggta ttccgcgcgc tatgaaccgg gctcgacagc gccgggatac gtcggtccaa
     2281 aaaggggcag cctgcgatcg gttcgcggcg ccggctggct gtcctttcag gccgccaccc
     2341 gatcggcggc acgtgatgcc gagaacccgg atcggttctt cattgatgtt ggtttcaggg
     2401 tcgtcgttcc agcccaatag cacgggcagg taactgttcc caagcgcaaa tgaactttat
     2461 tgggttctcg tggggctggt gcaaagctgg ctgacacaga tctgaccctt gttgcagtcc
     2521 ccatcggaca ggcagaacgg ataacggcga acctgctgtt cctggcatcc cccaagcgct
     2581 gccagcgcgg taagacaaag gatcgaaaga gccagaatcg tcgcaaaacg tttcatcata
     2641 aagtctccaa tccaccgaaa tcagttggtc ggcgaaggaa tcatgttaag catttcaaac
     2701 agggcgtcgg tcagcttgtt cctgataagg gcctcgtcgt tcgcgtaagt ttcgtatgga
     2761 atgtttgcct cgctgaccat cgcgtatccg ccgaattgtg gcagcagtac ggccttccag
     2821 tcttccggca ggacatctgt caacttgact tccttgaacc cggcccagat cacttcgccc
     2881 tggatgttga tcatccggat ttcaaccatg accgaccacc attcgcgcgg caggatcgag
     2941 ccctggccgg accacatcag gttgtaaccg tacttgtcgg gaacgggatt gaaaacgttt
     3001 tcgccgtttc tcagcgcata gatcttggtt tccttgaaaa ccttcatcgt gcgtacctgg
     3061 acgatggcat cggcgccggt ctccttgcca agaagaagcg ccttttcaag ggtgttccag
     3121 ctttcgcgcg tattgtggtt gttcgcgccc agatactgtt cgattctgga aaccacgccc
     3181 ttgtcaacca gaaggaagcc ctttgccatc agggaatgct cgacagcact gacgagcgcg
     3241 ttattcgcgg ccgaatcctt gtccagaagc gcactggctt ccggaagggc cgggccacgc
     3301 ctggccattc cccaaagctg gagaatgttg aatgccggct gctgggcgcc ctgggcgtag
     3361 tagtccggct ggtactgcgg ctgctgctga acctggggtt cggcacgctt ggtttcgtcg
     3421 ctggtcagaa gaagaacctt cgcgggtacc cgtgacaatc ccgaaacctg gtgaaccacc
     3481 ggctttgtcg gggccgtcct cttgatcatc atccgatatt cgccggcaca ccctgcaaga
     3541 accaagcaaa gcccgaaagt acccagaatg cttgaatacc tcataaccac ctcgcgaaaa
     3601 acaacattac ttaccgacac ggttttttct gttgccgctc gtcgtgatcc gtagaaatct
     3661 ggacatcgtg gtcggtgttg tctg
//
LOCUS       DAOPPA010000329         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27276_length_3682_cov_3.343259, whole genome shotgun sequence.
ACCESSION   DAOPPA010000329 DAOPPA010000000
VERSION     DAOPPA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27276_length_3682_cov_3.343259"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1749)
                     /locus_tag="PKH54_10015"
     CDS             complement(7..1749)
                     /locus_tag="PKH54_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00273.1"
                     /translation="MFSPLVSPVESEQSSFHKESMMNGFRNRLGCFALSLAIPMVALG
                     CGDDCDCPDGGAQCADAMVADTTSELPQDNGTELAPDVLADLREADAVTDAALDTSDS
                     ADIVPDTGSMEPVYWTAEATKTIEFDNFVKVDGKKFFGIGMHASPGKSYDGVTGPGEC
                     DRDTGRGYVDINIGKTRAGAAAGANFVYLWGYNERTTEILDVTPRFHGIFHRNYGTSP
                     APEDDVVPIIYNAFGESDMDGYSEERIAEMSSEFGEFITRSGKYSIESMPNLPPIEQV
                     GHMSWHPTFRMRGGGDGKGEVLPDWQVAEFAKTTNMMIGDAYAYVSNRWDTNDPDEAL
                     TAIITGQIGDIGEGYDHWIETDDEAHRSYFDSGFTLAESLVRNRNPDAVVWMWLQGYA
                     FGNSIKEAECEGEYSDSWAAGTFPPLDYLIKETLSVIAAGATGIVFFGYPSTSGPEAE
                     IMHTIFRGLSFPEVYEPALLSPRLDMGVNTTFMGEEGYDGRGRAHLAVKWDEAGRQAF
                     IIGANPGARATTVELEFPWSVAKVELLDWNVPRFRPTERVEVRNRKLLWTFDRDEGML
                     MRVTPLAAPAGSDD"
     gene            complement(1715..2323)
                     /locus_tag="PKH54_10020"
     CDS             complement(1715..2323)
                     /locus_tag="PKH54_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOD00274.1"
                     /translation="MEKISLRERKKTKTWESLVKISTRLFIEQGYDNTTVDQIVEKAE
                     VSQRTFFRYFPSKEAVMFRDYEKREQGLLRYLKEDEDGIEPYQRIKNALLGMANAFDQ
                     DRETMLAEYKIVMGSKHLIALDIEQDSRLERHVHAALREFRGRPFLCDRDAELVGGAI
                     FGSIRSALGYWFEGGCKADLAEISGQCLKIVDVLAAGFPGRI"
     gene            2504..>3682
                     /locus_tag="PKH54_10025"
     CDS             2504..>3682
                     /locus_tag="PKH54_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00275.1"
                     /translation="MSLFAIMVSFAAGCSSGQDSPDTIGDTVSDVMDNEIEPTDSGLD
                     LAVPDEGGTDLAIDAAPEVHVVPPCVMKIGVGKRDVSPVVEPFEDTNGNNWRDDGEAF
                     TDTNDNDEYDPTYIAGFGPRQAQGIHDPIWSRCMAIESTGQAYLFCATDSIGMGLSHY
                     DRIVARIKELRPQFNLEDSHVTWATTHTHQGPDTQGVWDLMPLEYLDFIDEQTAQAAV
                     EALDSMVPAGIRVVSAEQPDSLVRDIDVPVVIDRNISIIQGVAEDGDVIGTLVSIANH
                     PESGGPDNMKVSSDFPHYLRQKVEEVQGGMAIFFAGALGLMATPVMDWDNTGMWLTEQ
                     VGNAYGDIVNQALVDPPAPSCEYMFAAESRPSMKLENFDFWAGIESGLINGFIDYLYG
                     E"
BASE COUNT          721 a         1097 c         1056 g          808 t
ORIGIN      
        1 acgaaatcaa tcgtcactgc cggccggtgc cgcaagtggc gtgaccctca tcagcatccc
       61 ttcgtcgcga tcgaaggtcc agagcagttt gcggttccgc acctcgacac gttcggtcgg
      121 cctgaacctg ggaacgttcc agtcaaggag ttcaaccttg gccacggacc acggaaattc
      181 cagttccacc gtggttgccc gcgcaccggg atttgcaccg ataatgaacg cctgcctgcc
      241 tgcctcgtcc cacttgacag ccaggtgtgc ccgtcccctt ccgtcgtagc cttcctctcc
      301 catgaacgtg gtgttgacgc ccatgtcaag cctggggctg aggagcgccg gttcgtacac
      361 ctccgggaag ctgaggccgc ggaagatggt gtgcatgatt tcggcttccg ggccgctggt
      421 cgatggatat ccgaaaaata cgattccagt cgctccggca gcgattaccg acagggtttc
      481 cttgataagg taatcaagtg gcgggaaagt gccggcggcc caggagtccg agtattcgcc
      541 ctcgcactcg gcttccttga tactgttgcc gaacgcgtat ccctgcagcc acatccatac
      601 gaccgcgtcg ggattcctgt tcctgacaag cgactcggca agcgtgaacc cggagtcgaa
      661 gtagcttcga tgggcctcgt cgtcggtctc gatccagtgg tcgtagcctt caccgatatc
      721 gccaatctga cccgtgatga tggcggtcag ggcctcgtcc gggtcgttgg tgtcccagcg
      781 gttcgataca taggcgtatg cgtcgccaat catcatgttc gtcgtcttcg cgaattcggc
      841 cacctgccag tcgggaagga cctcgccctt gccgtccccg cccccgcgca tcctgaatgt
      901 cgggtgccag gacatgtggc cgacctgttc gatcggcggc aggttcggca tgctttcgat
      961 cgagtatttt cctgacctgg tgatgaattc cccgaattcg ctgctcatct cggcgatacg
     1021 ttcctcggag tagccgtcca tgtcagactc gccgaatgcg ttgtagatga tcggcaccac
     1081 gtcgtcctcc ggagccggtg acgtgccgta gttgcggtgg aagattccgt ggaaccttgg
     1141 tgtaacgtcg agtatctccg tcgttcgttc gttgtagccc cacagataga cgaagttggc
     1201 accggcggcg gcacctgcgc gggttttgcc gatgttgata tcgacatatc cccgcccggt
     1261 gtcacggtca cattcgcccg gcccggtaac gccgtcgtag gatttgcccg ggcttgcgtg
     1321 catgccgatt ccgaagaact ttttcccgtc aaccttcacg aagttgtcga actcgatggt
     1381 cttcgtggcc tcggcagtcc aatagaccgg ttccatggaa ccggtatccg ggacgatgtc
     1441 cgcggagtcg gatgtatcca acgcagcgtc tgtgaccgca tctgcttccc gaaggtcggc
     1501 cagaacgtcc ggcgccagtt ccgtcccgtt gtcctgcggc agttcggacg tagtgtcggc
     1561 caccatggca tcggcacatt gcgcgccgcc gtccgggcag tcgcagtcgt cgccgcaacc
     1621 gagagcaacc atcgggatgg ccagggaaag ggcgaaacaa cccagccggt tccggaaacc
     1681 gttcatcatc gactccttgt gaaatgaaga ctgctcagat tcgaccgggg aaaccagcgg
     1741 cgagaacatc gacaatcttc agacattgcc cgctgatttc agccaggtcg gccttgcagc
     1801 caccctcgaa ccagtaacca agcgccgatc gaatcgagcc gaaaatcgcg ccgccaacaa
     1861 gttcggcgtc acggtcgcaa aggaacggcc tgccacgaaa ttcccgcagg gccgcgtgaa
     1921 cgtgccgctc aagccggctg tcctgctcga tatccagtgc gatcaggtgc tttgaaccca
     1981 tgactatctt gtattcggca agcatggtct cgcgatcctg atcgaatgca ttggccattc
     2041 caagcagggc gttcttgatt cgctgataag gctcgattcc gtcctcgtct tccttcagat
     2101 agcgaagcaa tccctgctcg cgtttttcat agtccctgaa catcaccgct tccttgctgg
     2161 ggaagtaacg gaaaaatgtt ctctgggaga cttcggcctt ttcgacaatc tgatcgaccg
     2221 tcgtgttgtc gtatccctgt tcaatgaaaa gccgggtgga aatcttcaca aggctttccc
     2281 aggtcttggt cttttttctt tccctgagag atatcttttc caacttggat ttcctttagg
     2341 aagacaatca aaaatagatt gccttgctta taatgtccat gatagagatt gatgtcaggg
     2401 gctgtcaagt tacagattat gacagccttc ggaatttttt cagactgctc gaggttccaa
     2461 aatgctgaat caaaagggat ttatccgatc gggcgttgtg cgtatgtcgc tcttcgcgat
     2521 catggtttcg tttgcggcag gttgttcttc cggccaggat tctcccgaca cgattggtga
     2581 caccgtttcg gacgtgatgg ataacgaaat tgaaccgacg gattccggcc tggacctggc
     2641 agtcccggac gaaggcggca ccgatctcgc catcgacgcc gccccggaag ttcacgttgt
     2701 tccgccgtgc gtaatgaaga tcggcgtcgg caagagggat gtcagcccgg tcgtggaacc
     2761 gttcgaggac accaacggca acaactggcg tgatgatggc gaggccttta ccgacaccaa
     2821 cgacaatgac gagtacgacc cgacttacat cgcgggtttt ggcccgcggc aggcccaggg
     2881 tatccacgat ccgatctggt cccgttgcat ggcaatcgag agcaccggcc aggcctacct
     2941 gttctgcgcg accgactcga ttggcatggg gctttcccat tacgaccgca tcgtcgcgag
     3001 aatcaaggaa ctgcgcccgc agttcaatct tgaggacagc cacgtcacct gggccaccac
     3061 gcacacgcat caggggccgg acacccaggg tgtctgggat ctcatgccgc tcgaatatct
     3121 tgattttatc gacgagcaga ccgctcaggc ggcagtcgag gcgcttgact cgatggttcc
     3181 ggccggcatc cgcgtggtca gcgccgagca gcccgattcc ctggttcgcg acatcgacgt
     3241 gccggtcgtc atcgacagga acatcagcat catccagggc gtcgcggagg acggcgacgt
     3301 catcgggacg cttgtcagca ttgccaacca tccggagtca ggcggtccgg acaacatgaa
     3361 agtttccagc gatttcccgc actacctgcg ccagaaggtc gaggaagtgc agggcggcat
     3421 ggccatcttc tttgccggcg cgttgggcct gatggcgacg cccgtaatgg actgggacaa
     3481 caccggcatg tggttgaccg agcaggtcgg caacgcctat ggcgacatcg tcaaccaggc
     3541 ccttgtcgat ccgccggcgc cgtcgtgcga atacatgttc gcggccgagt cgcggccgtc
     3601 gatgaagctg gagaatttcg acttctgggc cggaatcgag tcgggcctga tcaacggatt
     3661 catcgattac ctgtacgggg aa
//
LOCUS       DAOPPA010000330         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27367_length_3672_cov_3.846834, whole genome shotgun sequence.
ACCESSION   DAOPPA010000330 DAOPPA010000000
VERSION     DAOPPA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27367_length_3672_cov_3.846834"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="hflX"
                     /locus_tag="PKH54_10030"
     CDS             complement(<1..1500)
                     /gene="hflX"
                     /locus_tag="PKH54_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOD00276.1"
                     /translation="MKARGNLVGLKQAATRQIEKLFERQASVEDFVGAPLAVAIQDAS
                     IACGRRVAVLADRGGRVFDVVVGDGTTIDLPADLRRRMPAGRLNGFRLIVSTFQAGLG
                     LDDLETMRLYSFDAVVAVVNGQPGTTPVVQPAWPLPPGSGDSFWEILAPAYPGRFVET
                     FDTTIRDTETQLRRAASDNLLSPGGRKARDAAIVVIPSFSRQDDLEWERSEMEALCYT
                     AGVAVIETVVQRRQEPDPRYVTGVGKLKEVALKCLHGAADLIIFGVTLTPSQQRSISE
                     VAGVRVLDRNQLILDIFARNAASAEGRLEVELAQLRYNLPRLSERDDAMSRLSGGIGA
                     LGPGETKLEMERRRARDRIHQLEQRILQEAGRRDLRRRKRGRQAEPLVAIAGYTNAGK
                     STLFNALSGADVVARDKLFATLDPTVRNVWLPPGANCLMVDTVGFIRDLPKELEGAFS
                     ATLEEIGRADIILQVADASDPHVVVQIQSVRRILGEMGHGEIPTVLALNK"
     gene            complement(1497..2870)
                     /locus_tag="PKH54_10035"
     CDS             complement(1497..2870)
                     /locus_tag="PKH54_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD00277.1"
                     /translation="MELKVLVIDDEPGLLQMLQVVLGRAGYAVTTVTSAVEGLQLLER
                     NRFDLCLCDLRMPGMDGMGFLNVLKSQGVALTVIVMSAYGSGDDAIAAMKAGAYDYIN
                     KPFQSDEILLTLRKAVEREQLKRENIELRSKVSRQAGVPSVIARSESMKRVVAVAARI
                     AVFKSTVLITGESGTGKEVIARLIHEASDRSRKPFIAVNCGAIPEGLMESEFFGHVKG
                     AFTDAIGNRRGLIESAGGGTLFLDEVGELPPILQVKMLRFLQESEVRKVGDTKPVKVD
                     VRVVAATSRDLEQEIAKGRFREDLFYRLNVVPIKIPPLRTRLEDIPPLADHFLQRMKE
                     RTSCSADSFAPDAMRALMNHQWPGNVRELENIVERVAVMCDSQVVTAADLPDSVRMLS
                     PCDPAISLPLEGLSLKVNQRALEISLISRALEATGGNRTHAARILEMSHRALLYKLKE
                     YGLGKAE"
     gene            3013..>3672
                     /locus_tag="PKH54_10040"
     CDS             3013..>3672
                     /locus_tag="PKH54_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00278.1"
                     /translation="MRGRIILSILFAGALAVFGLGPFLRSMILPVLVNNEGDRLMAAA
                     EMAAVSIPAGQPTQWSSRIRKAWPGVTVFVIDVGPDRDGGNASLSERAARHQTSPERL
                     IKASIAGKLLTSIDPSSPDRQASGLDAGWEALVRIPSESGSMAVLGMRLGQFKAAESR
                     LWKLVTLYGILVTILSLTGGLIVAFLTIVRPIGRSAAAATRLLEGDSAGAGMTDLQRI
                     DS"
BASE COUNT          699 a         1187 c         1076 g          710 t
ORIGIN      
        1 cttgttcaac gcgagcaccg tcgggatttc gccatggccc atctcgccga gaatgcgcct
       61 gaccgattga atctgaacca cgacgtgcgg atccgacgcg tcggcgacct gaaggatgat
      121 atcggcgcga ccgatctcct cgagggtcgc gctgaaagcg ccttccagtt ccttcggcag
      181 gtctcggatg aaaccgaccg tatcgaccat caggcagttc gcgccaggag gcagccacac
      241 gttcctgacg gtcgggtcga gcgtcgcgaa cagcttgtcc ctggcaacca cgtcggcacc
      301 gctgagggcg ttgaacagcg ttgacttgcc tgcgttcgtg taccctgcga tcgcaaccag
      361 gggttccgcc tggcgacccc ttttccggcg cctcagatca cgccgaccgg cctcctgaag
      421 gatcctctgc tcaagctgat ggatgcggtc gcgcgccctt ctgcgttcca tttcgagctt
      481 cgtttcgccc ggtccgagcg cgccgatgcc cccggacagc ctggacatcg cgtcgtcccg
      541 ttccgacaaa cgcgggaggt tgtacctcag ctgggcaagc tccacttcca ggcgaccttc
      601 cgcggaagcg gcattacggg cgaaaatgtc caggatgagc tggttacggt cgagaaccct
      661 gaccccggcc acctcggaga tgctgcgctg ctgcgacggg gtcagagtaa ccccgaagat
      721 gatcaggtcg gccgccccgt ggaggcactt gagggctacc tccttcagct ttccgacacc
      781 ggtgacgtac ctcgggtcgg gctcctgccg tcgctggaca accgtctcga tgacggccac
      841 gccggccgtg tagcacaagg cctccatctc gcttcgttcc cattccagat cgtcctgcct
      901 ggagaatgat ggaataacca cgatggcagc atcgcgcgcc ttccgtccgc ccggggacag
      961 caggttgtcc gatgccgccc tgcgaagctg tgtctccgtg tcgcggatcg tcgtgtcgaa
     1021 ggtttcgacg aaccgaccgg gataggccgg cgccagaatt tcccagaagg aatcgccgga
     1081 tcccggggga aggggccatg ccggctgcac gaccggtgtc gtgccgggct ggccgtttac
     1141 gaccgccacg accgcatcga aggaatacag ccgcatcgtt tcaaggtcgt ccaatccgag
     1201 gcctgcctgg aatgtcgata caatcagcct gaagccgttc agacggccgg ccggcatgcg
     1261 acgcctcaga tccgctggaa ggtcgatcgt cgttccgtcc ccgacgacca catcgaatac
     1321 cctgcccccc cgatctgcca gaaccgcaac cctgcggcca caggcgattg aagcgtcctg
     1381 gatcgcgacg gcaagcgggg ccccgacgaa atcctcgacc gaggcctggc gctcgaacag
     1441 cttctctatc tgccttgtcg cggcctgctt aaggccgacc aggttgccac gcgccttcac
     1501 tcggccttcc ccagcccgta ttccttgagc ttgtaaagca acgcccggtg actcatttca
     1561 agtatcctgg ccgcgtgcgt gcggtttccg ccagttgctt cgagcgcacg gctgataagc
     1621 gaaatttcaa gggccctctg gttgaccttc agcgaaagcc cctcgagcgg aagcgatatt
     1681 gcgggatcgc agggcgacaa catccttacc gaatcgggga gatcggccgc ggtgaccacc
     1741 tggctgtcgc acatgacggc aacccgttcg acgatgttct ccagttcccg gacattgcct
     1801 ggccactgat gattcatcag cgccctcatt gcatcgggag cgaacgagtc ggcactgcac
     1861 gaagtccgct ccttcatcct ctgaaggaaa tgatccgcaa ggggcggaat atcctcgagc
     1921 ctggtccgca ggggcggaat ctttatcgga acgacgttga ggcggtagaa aagatcctcg
     1981 cggaagcgac ctttcgctat ttcctgctca aggtcgcgcg acgtggccgc gacgacccgc
     2041 acgtcaacct tcaccggctt cgtgtcgcct accttgcgga cctcgctctc ctgaaggaaa
     2101 cgcagcatct tgacctgaag gatcggcggc agttcaccga cttcatctag gaaaagggtg
     2161 ccgcccccgg cgctttctat cagtccgcgg cgattgccga tcgcgtcggt gaaagcaccc
     2221 ttgacatgcc cgaagaactc ggactccatc aagccttccg gaatcgcgcc gcagttgacc
     2281 gcgataaacg gctttcggga tcgatccgac gcctcgtgaa tcaggcgcgc gatgacttcc
     2341 ttgcctgtgc cggattcgcc cgtgataaga accgttgact tgaagacggc aatcctggcg
     2401 gcgaccgcga ccacgcgctt catcgactcg cttcgggcaa tgacggacgg gacgccggcc
     2461 tgcctggaca cctttgacct gagttcgatg ttttcccgct tcaactgttc gcgttcgacc
     2521 gccttccgga gggtcaggag tatctcgtcg gactggaacg gcttgttgat gtagtcgtat
     2581 gcgccggcct tcatcgcggc gattgcgtca tcacccgaac cgtacgccga catcacgatt
     2641 acggtaagcg caacaccctg tgatttcaat acgttgagaa agcccatgcc atccattccc
     2701 ggcatccgga gatcacagag gcacaggtcg aaccgattcc gctcgagcaa ctggagaccc
     2761 tcgacggcgg acgtaacggt tgtgactgca tagccggccc tgcccaggac gacctggagc
     2821 atctgaagca atcccggttc gtcgtcaatc acaagaactt tcagttccat acaaccccgc
     2881 aggcggcgcc gggaacatta taatccggta tgatcccgtt cgaccggaaa atctggtaag
     2941 ttaggccggt cattactcaa gccggaccgg tcgcagatgc tgggatcgac gggaaaattt
     3001 ccgggtaccg gggtgcgcgg acgaatcatt ctgtcgattc tgttcgctgg cgccctggcc
     3061 gttttcggcc tgggaccttt cctccgatcc atgattctcc cagtccttgt aaacaacgaa
     3121 ggggatcgcc tgatggctgc ggccgaaatg gccgcggtct ccataccggc cggacagccg
     3181 acccagtggt cgtcccgaat cagaaaggcc tggccggggg tcacggtttt tgtaatcgat
     3241 gtcgggcccg accgtgacgg cggaaacgca tccctgtccg agagggccgc aaggcaccag
     3301 acatccccgg aaagactgat caaggcaagc attgccggaa aactgctgac ttccatcgac
     3361 ccgtccagcc cggacaggca ggcatccggc ctggatgccg gctgggaagc cctggtcagg
     3421 attcccagcg agtccggttc gatggcggtg ctcggcatgc gccttggaca attcaaggcg
     3481 gcggaatcaa ggttgtggaa gcttgtgacg ctttacggaa tcctggtcac gatcctgtcc
     3541 ctgacaggcg gcctcatcgt cgccttcctt accatcgtcc gcccgatcgg cagaagcgct
     3601 gccgcggcca ccaggcttct cgaaggcgat tccgccggcg cgggcatgac cgatcttcaa
     3661 cgaatcgact cg
//
LOCUS       DAOPPA010000331         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27697_length_3637_cov_3.798716, whole genome shotgun sequence.
ACCESSION   DAOPPA010000331 DAOPPA010000000
VERSION     DAOPPA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27697_length_3637_cov_3.798716"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PKH54_10045"
     CDS             complement(<1..1443)
                     /locus_tag="PKH54_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00279.1"
                     /translation="MDGARIERFSWKDFRRVAGRVAAARGPAVGRYGMVVLLSLVFLA
                     PGCGGDGSPDDPCRGVTCSGQGECVSVAGEPVCDCNAGFHANGTACVPDACRPADCVH
                     GNCVDPLNLPDCECDEGYAGTACDQCDEGYVLDGTDGLRCVLDASCDEDPCGEHGVCR
                     TEGAGVACTCDDGWDGNLCNRCADGYHDESGACLPDTPCNPDPCVHASKCDEVAGEAR
                     CTCLNGYDGDLCDDCAEGFVKDGLVCVAGPGPCDPNPCPGLNPGEHRDRCTVEEGAAV
                     CLCDFGYEEVEGDCVQQTVCDPVTTCAGHGTCTGNGLECKCDDGYKGDNCDECDQGFE
                     AQGDDCVPVQPPDPCDPNPCGAEPDRARCVVEGGAAVCACDQGFDDVAGVCVPKCDVH
                     ETACAKAHSSFGWIVSANGHGALVVNLDSTKLPAGDDRDNHRYMLFVHPYQTWGLNGS
                     GQAVNTRDMLYDTFMGLRVDGVGTWLDGVPR"
     gene            complement(1443..>3637)
                     /locus_tag="PKH54_10050"
     CDS             complement(1443..>3637)
                     /locus_tag="PKH54_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD00280.1"
                     /translation="CLCDDGYRLDQSDTCVEDTGSLETRSCDVVVSTQVSTSGPVALR
                     GEFNGWSETADVMTKVGAKWERTMSGLRAGDYAYKLFWREGGKERWELDPDNPYTKWV
                     GGTRNSMLRVPDCDKPQLVLEQGPAVNGGRVTMKVRAMYGRQRIELDAAQATVTRNGT
                     SISGTWDGDTGTFTIDDSGLEPGKYGYVFQVADTAGRRAEPLFVPLWVEDQAFDWRDA
                     TIYFVLTDRFHNGNTGNDLPVSDPKLDFKANWQGGDFAGLKQKIDAGYFDDLGVNCLW
                     VSSPVMNTQGAFWGSDGHKYTGYHSYWPIATGWTGDTPLTGLAGPIDPHFGDMEAFKA
                     MVAAAHARGIRVIVDFVANHVHTDSPLYTQHRNDSTPWFNWNNGTVGQGYVCGWDKPI
                     ECWFAEYLPDFDYRNGQVMDRVMDHAIWLIMETNIDGFRLDAVKHMILDFSATLRGRI
                     DDQIDTYDGIRFYMVGETFTGEGDGEKQTIKEYVSPDLLDGQFDFPMFWAVLAALVRH
                     ERGLDSLKSFMDGNDGYYGAAAVMSTFLGNHDVPRALSHAAGQIGDLWGNGSKEQGWT
                     APPGQPSAEDPYRRLRMAWTFLFSQKGLPLLYYGDEFGMPGAGDPDNRRFMRFDSALS
                     TNEKATLSHVQALGKARLRHPALRRGSRANIQVDANYWAFVMKDGTDAVLVVLNRGDA
                     GARTLAVGAAGLPGGTYRDVLSGREISVSGGSASVSMGRLESAVFEVK"
BASE COUNT          670 a         1302 c         1025 g          640 t
ORIGIN      
        1 ccggggcacg ccgtccagcc aggttccgac gccatcgacc cgcaagccca tgaacgtgtc
       61 gtacagcatg tcacgggtat tgaccgcctg gccgctgccg ttcagtcccc acgtctggta
      121 cggatggaca aacagcatgt agcggtgatt gtcgcgatca tcaccggccg gcagtttcgt
      181 cgagtccagg ttgaccacca gcgcgccgtg accgtttgcg gaaacgatcc acccgaaact
      241 ggaatgggcc ttcgcgcagg cggtctcgtg aacgtcgcac ttcggcacgc acacgccggc
      301 cacgtcatcg aaaccctgat cgcacgcgca gacggccgcc ccgccttcaa caacgcacct
      361 ggcacggtcc ggttccgcgc cgcacggatt cgggtcgcac ggatcgggcg gctgcaccgg
      421 cacgcagtcg tcgccctggg cctcgaatcc ctggtcgcat tcgtcgcagt tgtcgccctt
      481 gtagccatcg tcgcacttgc attccagccc gttgccggta catgttccgt ggcccgcgca
      541 tgttgtcacg gggtcgcata ccgtctgctg gacgcagtcg ccctcgactt cctcataacc
      601 gaagtcgcac agacaaaccg ccgcgccctc ttcaacggtg cagcgatcgc gatgctcgcc
      661 gggattcaat ccgggacacg gattcgggtc acacggcccc ggcccggcaa cgcagaccag
      721 gccgtccttc acgaagcctt ccgcgcagtc gtcacaaagg tcgccgtcat atccgttcag
      781 gcacgtgcac ctggcctcgc ccgcgacctc atcgcacttc gacgcatgca cgcacggatc
      841 gggattgcac ggggtatcgg gaaggcacgc accgctttcg tcgtggtatc cgtcggcgca
      901 acggttgcaa agatttccat cccagccgtc atcgcatgtg caggccaccc cggcaccttc
      961 cgtgcggcaa acgccatgtt cgccgcatgg atcttcgtcg cacgacgcgt ccagcacgca
     1021 ccgcaacccg tcggtcccgt ccagcacata gccttcgtca cactggtcgc atgccgtgcc
     1081 ggcatatcct tcatcacatt cgcaatcggg aaggttcagg ggatccacgc aattcccgtg
     1141 cacgcaatcc gccggacggc aggcgtccgg cacgcaggcg gttccgttgg catggaatcc
     1201 ggcattacag tcgcacaccg gctcgcctgc cacactcacg cattcgccct ggccggaaca
     1261 tgtgacaccg cggcatggat catcggggga cccgtcgccc ccgcaaccgg gggccagaaa
     1321 caccagcgac agcaggacga ccatgccgta ccgaccgacc gccggaccac gggcggcagc
     1381 cacccgaccg gcgacccgcc ggaaatcctt ccaggaaaac ctttcaatcc ttgccccgtc
     1441 catcacttca cctcaaaaac ggccgattca aggcggccca ttgaaacgga cgccgaaccg
     1501 cccgacaccg aaatttcccg accggacaag acatccctgt acgttccgcc aggcaggccg
     1561 gccgcgccga ccgccagcgt acgggccccg gcatccccgc ggttcaggac caccaggacg
     1621 gcatccgtcc cgtccttcat gacaaaggcc cagtaattcg catcgacctg gatgttcgcg
     1681 cgcgaaccgc gccgcagcgc cgggtgccgt aacctggcct ttccaagcgc ctggacgtgc
     1741 gacagcgtcg ccttttcgtt tgtcgaaagc gccgaatcga aacgcatgaa ccgccggttg
     1801 tcagggtcgc ccgcccccgg catgccgaac tcgtcgccgt aatacagcag aggcaacccc
     1861 ttctgggaaa acaggaaagt ccacgccatt ctcaggcgcc ggtacgggtc ctcggcggaa
     1921 ggctgcccgg gaggcgccgt ccacccctgc tccttggagc cgttgcccca cagatcgcca
     1981 atctgcccgg cggcatggga aagcgcgcgc ggcacgtcgt ggtttccaag gaacgtggac
     2041 atcaccgccg cggccccgta atagccatcg ttgccgtcca tgaacgactt cagcgaatcc
     2101 agcccgcgct cgtgccgcac cagtgccgca agcaccgccc agaacatcgg gaagtcgaac
     2161 tgtccgtcca gcagatccgg actgacgtac tccttgatcg tctgcttttc gccgtctccc
     2221 tcgccggtga aggtctcgcc gaccatgtag aacctgatgc cgtcgtacgt gtcgatctgg
     2281 tcgtcaatcc gcccccgaag cgtcgccgaa aagtccagga tcatgtgctt gaccgcatcc
     2341 agtctgaagc cgtcgatatt ggtctccatg atcagccaga ttgcgtgatc catcacccga
     2401 tccatgacct ggccgttccg gtaatcgaaa tcgggcagat actccgcgaa ccagcactcg
     2461 atcggcttgt cccacccgca cacgtacccc tggccaacgg ttccgttgtt ccagttgaac
     2521 catggcgtcg agtcgttccg gtgctgcgtg taaagcggcg agtcggtgtg cacgtggttc
     2581 gccacgaaat cgacgattac cctgattccg cgggcgtggg cggccgcgac catcgccttg
     2641 aacgcctcca tatccccgaa gtgcgggtcg atcggtcccg ccaggcccgt caacggagtg
     2701 tcgccggtcc aaccggtggc gatcggccag tacgaatggt acccggtata tttgtgcccg
     2761 tcggaccccc agaacgcgcc ctgggtattc atgacgggcg acgacaccca caggcagttc
     2821 acccccaggt cgtcgaaata gcccgcgtca atcttctgtt tcaggccggc gaagtcgcca
     2881 ccctgccagt tcgccttgaa gtccagcttc ggatccgata ccggcaggtc gttgccggtg
     2941 ttcccgttat ggaagcgatc cgtcagcacg aaatagatcg tcgcatcgcg ccagtcgaac
     3001 gcctggtcct cgacccacag gggcacgaac aacggttccg cccgacgacc agccgtgtcg
     3061 gcaacctgga agacatagcc gtacttcccc ggctccaggc ctgaatcgtc gatcgtgaaa
     3121 gtgcccgtat cgccgtccca ggtgccggaa atcgaggttc cgttgcgggt cacggtggcc
     3181 tgcgcggcat ccagctcgat cctctggcgg ccgtacatgg cccgcacctt catcgtcacg
     3241 cgcccgccgt ttacggccgg gccctgttcc aggacaagct gcggcttgtc gcagtcaggc
     3301 acgcgcagca tcgagttccg cgtcccgccg acccacttcg tgtacggatt gtcaggatcc
     3361 agttcccagc gctccttccc gccctcgcgc cagaacagct tgtatgcata atccccggcg
     3421 cgcagtccgg acatcgtcct ttcccatttc gcgccgacct tcgtcatcac gtcggccgtc
     3481 tcggaccagc cgttgaactc gccacgcaag gccacgggcc ctgaagtcga aacctgcgtg
     3541 ctgacgacga cgtcgcagga tctggtctcg agcgagccgg tatcctcgac acaggtatcg
     3601 gactgatcca ggcgataccc gtcgtcgcaa aggcatg
//
LOCUS       DAOPPA010000332         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27772_length_3629_cov_4.363738, whole genome shotgun sequence.
ACCESSION   DAOPPA010000332 DAOPPA010000000
VERSION     DAOPPA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27772_length_3629_cov_4.363738"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKH54_10055"
     CDS             complement(<1..468)
                     /locus_tag="PKH54_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00281.1"
                     /translation="MTPEDIRVLLDQVASGRMTPADALLELRDLPARRLGDVAWDSHR
                     ILRRGIAESIYGSTKTPRQIADALLSSSAAGTNCVVTRIKPEPATDAIELFVADGGKG
                     ELVHHEPGSILSLLVDASRPISDGFVAVVTAGTPDIPVAEESAVTLETCGYRVV"
     gene            660..2204
                     /locus_tag="PKH54_10060"
     CDS             660..2204
                     /locus_tag="PKH54_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00282.1"
                     /translation="MRDFPESGPDPLVRPDGLHNGVAAPMVPGEVVNNENPEDVFERV
                     VIWQPWSYPVPVDVLVEGMSDVDRGFIMAVYRLAQNLYGQIPMRKNGEAAFTHPTNVA
                     MYLKRARCQPYVIAAGLLHDFIEERVDLEKAAIRSEIAARIKAEVTSRINARIRAELT
                     ASMNSFDVRDIAGADSTDRVETARRQVRVDLATQTRAAIAQLDASILLNDEIGELVKQ
                     EMGRVSLSDLENAIRGDFGREINRITDRTGFPRHVAARVVETVWLLTRHKSDLYYRSI
                     SGIFINSDLTIRVAAALVKLADRMHNIQTIDNYESSEKLYQCFKNLFILNNSQQLLAT
                     RSESMDWRMSYSLDKMFKKSGKALFEALHNLAHTADSSQKVFKVVSYLALALRKYIHE
                     QDGLARVNQECACPGAPVHAIFDGIVIKYDHRLHGEMDAFLQQQDRELGYMRATFGTL
                     GLSDDDLREAVAYKDAMALLEVVGMLVYRKDFMILDFQCSALCRRGQMCTRRAAGPEN
                     SGSEQD"
     gene            2240..3118
                     /locus_tag="PKH54_10065"
     CDS             2240..3118
                     /locus_tag="PKH54_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOD00283.1"
                     /translation="MSATIPSVDEFVELLVVASSDLPDDVEAAIGRALLLSGGTAGCI
                     LSEMRDNSELARVRRQPVCQDTGIPYVLATGLGLDSSGRRDFKARLAEAIRICTSSGV
                     LRPNAVDALTGVNPGDGIGEGIPQVHFEDEGCERATVDVLLKGGGSENVGCQYSLPDQ
                     SIGAGRDLEGIRRCIIDAAFRAQGLGCSPGILGVCVGGDRASGYEFAKRQLFRRLDHP
                     SSGAFAALESELVDQINSLGIGPMGLGGSPTVLGVRIGSLARHPASFFVTVAYGCWAL
                     RRRRLLASPDGVWVVV"
     gene            3111..>3629
                     /locus_tag="PKH54_10070"
     CDS             3111..>3629
                     /locus_tag="PKH54_10070"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOD00284.1"
                     /translation="MSDGFCSGGGSVNFEANQVRLQSPGDRDVIRSLRVGDRVLLSGF
                     IVTGRDLVHRMLARDLPDSVRPLLRNAFIYHCGPVAVRDGAGWRIVSAGPTTSMREEP
                     YQAGILKEYGLLGVIGKGGMGRATSIALAETGGVYLHATGGAGSLLASRIVGVPDLRY
                     LAEFGVPEAMWVF"
BASE COUNT          690 a         1021 c         1162 g          756 t
ORIGIN      
        1 gacgacgcgg tatccacaag tctcgagcgt taccgccgat tcctcggcaa ccggaatgtc
       61 gggcgttccg gccgtaacga cggcaacaaa gccatcggaa atcggccgac tggcatcgac
      121 cagaagcgac aggatggagc ctggttcgtg gtggacaagt tcgcccttgc cgccatcggc
      181 gacaaaaagt tcaatggcgt ccgtggccgg ttccggcttg attctggtca ccacgcagtt
      241 ggttccggcc gccgaggaag acagcagcgc atcggcgatc tgcctcggtg tcttggtcga
      301 accgtagatc gattcggcga tcccccgtcg aaggatgcgg tgcgaatccc atgcgacgtc
      361 gcccagcctc cttgcgggaa gatccctgag ttccagcaac gcgtcggccg gagtcatcct
      421 gccggatgcg acctgatcaa gcagaaccct gatgtcttcc ggggtcatcc agtttccaga
      481 ttcgagccag tggaattctg gacttgcgat ggcatcgcgg tcaagtgcgg acagcggtcg
      541 attaattgaa ttcccgcggt cggggcgtat aatcgaaact ggtcgctttc gggttgtgcc
      601 cgcgaatttc gtgcgcggca gtttcacaac cacatctgtt ttgggggatt ggtcagggta
      661 tgcgtgattt tccggaaagc gggcccgatc cgctcgtcag gcccgatgga ttgcataacg
      721 gggtggccgc gccaatggtg ccgggggagg tcgtaaacaa cgaaaacccg gaagacgtgt
      781 tcgagcgggt cgtaatctgg caaccatggt cgtatccggt tccggtcgat gtcctggtcg
      841 agggaatgtc cgacgttgac cgcggattca taatggccgt ctatcggctt gcccagaatc
      901 tttacggcca gattccgatg cggaagaacg gcgaggcagc cttcacccat ccgacaaacg
      961 tggcgatgta cctgaagcgg gcgcgttgcc agccttatgt catcgcggcc ggcctgcttc
     1021 acgacttcat tgaggaacgg gtcgatctcg aaaaggcggc gataaggtcc gaaatcgccg
     1081 ccaggataaa ggccgaggtg acctcccgga tcaacgccag gatccgggcc gaattgaccg
     1141 cttctatgaa ctccttcgac gtgcgggaca tcgccggcgc cgattcgacc gatcgcgtcg
     1201 agaccgccag gcgccaggtc agggtcgatc ttgccaccca gaccagggca gcgattgccc
     1261 agctcgatgc atcgattctt cttaacgacg aaattggcga actcgtgaag caggagatgg
     1321 gccgggtttc cctttccgac ctggagaacg cgatacgcgg ggacttcggc agggaaatca
     1381 accggattac cgatcgcacc gggtttccgc gccatgtcgc tgcccgtgtg gtcgagacag
     1441 tatggcttct tacaaggcac aagtccgacc tttattacag gtcgatctcg ggaatcttca
     1501 taaacagcga cttgaccatc agggtcgcgg cggcgctggt caaactggct gaccggatgc
     1561 acaacatcca gacgatcgat aactacgaaa gcagtgaaaa actttaccag tgcttcaaga
     1621 accttttcat cctgaacaat tcgcagcagc ttctggccac caggagcgag tcgatggact
     1681 ggcggatgtc gtattcgctg gacaagatgt tcaaaaagtc aggaaaggcg ctgttcgagg
     1741 cgcttcacaa cctggcgcac acggccgatt cgagccagaa ggttttcaag gtcgtttcgt
     1801 atcttgctct tgcgttgcgc aagtacatcc acgagcagga cgggctcgcc agggtcaacc
     1861 aggaatgcgc gtgccccggg gctccggtac atgcgatttt tgacggcatc gtcatcaagt
     1921 acgatcacag gctgcacggc gagatggacg ccttcctgca gcagcaggac agggaactcg
     1981 gatacatgag ggcgaccttc gggactcttg gcctgagcga cgacgacctg cgcgaggcag
     2041 ttgcctacaa ggacgcgatg gcgttgcttg aagtcgtcgg gatgcttgta tatcgcaagg
     2101 atttcatgat cctggacttc cagtgctcgg ccctttgcag gcgcgggcag atgtgtacca
     2161 ggcgggccgc cggccccgaa aattccggct ccgaacagga ctagccggaa ttcaggccgg
     2221 cgttgccccg ggagttatgg tgtcagccac gatcccttcc gtcgatgaat ttgtggaact
     2281 tcttgtggtg gcctcgtccg atctgccgga tgacgtcgag gccgccatcg ggcgggcgct
     2341 gcttttgtcc ggcggcacag ccggctgcat actttccgag atgcgcgata attcggaact
     2401 ggcccgcgtc aggcgtcagc ccgtctgcca ggacaccggg attccgtacg tccttgcaac
     2461 cgggctcgga cttgattctt cagggcgtcg ggattttaag gcaaggcttg ccgaggcaat
     2521 acgaatctgc acgtcaagcg gggtgctgcg ccccaacgcg gttgacgcgc tgaccggcgt
     2581 caatcccggt gacggaatcg gcgagggcat tccccaggtg catttcgagg atgaagggtg
     2641 cgaacgggcg acggtcgatg tgctgctgaa gggcggcggc agtgagaacg ttggctgtca
     2701 gtacagcctt ccagatcagt cgattggggc agggcgcgac ctggaaggga tccgtcgatg
     2761 catcatcgac gccgccttcc gggcccaggg gctgggctgc tctccaggca tccttggcgt
     2821 ctgcgtcggc ggagatcggg catctggata tgaattcgcc aagcgtcagc ttttccggcg
     2881 cctggatcat ccttcgtcag gagcttttgc ggcccttgaa tccgaactgg tcgatcagat
     2941 caattcgctc gggatcggtc cgatggggct cggcggcagc ccgactgttc tgggcgtccg
     3001 gataggttcg cttgcccggc acccggcaag tttcttcgtt acggtcgcat acgggtgctg
     3061 ggcgcttcgt cgaagacggc tcctggcaag tcccgacgga gtctgggttg ttgtctgacg
     3121 gtttctgttc tggcggggga agcgtgaatt tcgaggcaaa ccaggtcagg ttgcaatcgc
     3181 cgggggacag ggatgtcatc cgttccctgc gggtcggtga tcgcgtgctg ctttccggtt
     3241 tcatcgtcac cggcagggat cttgtccacc ggatgcttgc cagggacctt ccggattcgg
     3301 tgcggccgct gcttcggaac gctttcatat atcattgcgg gccggtcgcg gtcagggatg
     3361 gtgccgggtg gcgaatcgta tccgccggtc cgaccacgtc catgagggaa gaaccttacc
     3421 aggccgggat tcttaaggaa tacgggctgc tgggcgtaat cggcaagggc ggaatggggc
     3481 gggcgacgtc gatcgcgctt gccgaaacag gcggcgtcta tcttcacgct accggcggcg
     3541 ctggctccct gcttgcgtca aggattgtgg gcgttcctga cctaaggtac ctggcagaat
     3601 tcggtgttcc cgaggcaatg tgggtcttc
//
LOCUS       DAOPPA010000333         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27859_length_3620_cov_4.638990, whole genome shotgun sequence.
ACCESSION   DAOPPA010000333 DAOPPA010000000
VERSION     DAOPPA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27859_length_3620_cov_4.638990"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKH54_10075"
     CDS             <1..381
                     /locus_tag="PKH54_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOD00285.1"
                     /translation="TYGMPEVPAVGISIGVDRLFAALAELGQAPMGGAGPRAVVCTLG
                     DRALDSALGLVSELRDAGISCEAAPEPGAKLKRQLAHAVRRGARYAILLGEDEVGQGV
                     VTVKNLATGEQGQVERNQVASALV"
     gene            397..1050
                     /locus_tag="PKH54_10080"
     CDS             397..1050
                     /locus_tag="PKH54_10080"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HOD00286.1"
                     /translation="MARDSDLEQELADIAALTERLRVHFNAYFLGLEKNAPMNLRDQL
                     ERRIRTSILNDECKAFYKFRFQAAVSRYRTMSVYWDRILRDLETGRTSRAKLRAEKGY
                     GAAAVENIETPRMRRIREAAAKNAAGADGGDGGDGGDGTAGTQSGPAHGRASLYEQFI
                     QARQSVGLPTEGITPEGFARTLAKQKQIHTANPEIRDVEFSVKVKEGRVVVVARPVK"
     gene            1089..2405
                     /locus_tag="PKH54_10085"
     CDS             1089..2405
                     /locus_tag="PKH54_10085"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOD00287.1"
                     /translation="MLFSQQIRFKRDGRALQPEAIRSFIREYASDMIPDYQMAAMLMA
                     VFFKGLNPDELWAWTDAMLRSGDVMDFSDIPGAKVDKHSTGGVGDKISIPLAPLVAAC
                     GVNVPMISGRGLGHTGGTLDKLESIPGFKTRIGTNEFRAALAANGVAMLGQTENIVPA
                     DKRMYSLRDVTGTVESIPLIAASIMSKKLAEGISGLVLDVKVGSGAFMKGVDQARELA
                     GTLVAVGQAGGVRTTAFLTDMDEPLGTMVGNALEIVESVQILRNEGPADTTALTVELG
                     ARMLLLGGVVVSHEEGRERILGAIASGAGLDKFRSMIAAQGGNPAVADDLSILPRAAR
                     QIGVKASRNGYVTAVDCEKVGIASLLVGGGRRRKEDEIDPAVGVRILARRGTFVSVGD
                     DVAVIEYNSDASLDAASKLLSSAFSIGDEMVPAGPLVIDVIDGAAL"
     gene            2422..3603
                     /locus_tag="PKH54_10090"
     CDS             2422..3603
                     /locus_tag="PKH54_10090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD00288.1"
                     /translation="MKFEYSNPVQVVFGPGRISELGKLTAGIGNRAVVVIGGGSVRNN
                     GTLDKAVKSLEAARVAIHVFEGVEPNPRISTVRRGIDAIRAFRGNVVIALGGGSVMDA
                     AKVMAAGALYDQDPWKMCKVSQAKPVAPTASLPVITVPTLAATGSEMNCNAVITNWEA
                     NMKAAAIYPILYPKLALIDPELTVTVPKYHTAAGVVDIITHVTEGYFNAQPGTPLQDR
                     MAEAVISTAIEHGARAVADGSDLAAREAVMWASTVALNNWIHVGWNAPFPCHGIEHVL
                     SAHYDVSHGAGLAAISPSWMRFVVRRVGPGKWAGFARRVMAVDCADDVEAALLGIDRL
                     EEFFASIGAPTRLSGLGIPAAEIRRLAEDTVRVFGVNGAIAGVPPLTVDDIESILNSA
                     A"
BASE COUNT          661 a         1035 c         1228 g          696 t
ORIGIN      
        1 acttatggca tgcccgaggt tccggccgtc ggaatcagca tcggcgttga caggcttttc
       61 gcggcgcttg cggaacttgg acaggccccg atgggcggtg ccgggccgcg cgcggtggtg
      121 tgcaccctgg gtgacagggc cctggattcg gcccttggcc tggtgtcgga acttcgcgac
      181 gccggcatct cgtgcgaggc ggcccccgag cctggcgcca aactgaagag gcagcttgcc
      241 cacgctgttc gtcggggcgc cagatatgcg atacttcttg gcgaagacga ggtcggtcag
      301 ggcgtcgtga ccgtgaagaa ccttgccacc ggtgaacagg gccaggtgga acgtaaccag
      361 gttgcttcgg cgctggtttg atgcaggtgt cggaagatgg ctcgggacag cgatcttgaa
      421 caggaactgg ccgacatcgc ggcattgacc gaacggttgc gcgttcactt caatgcgtac
      481 tttctgggcc tggaaaagaa tgccccgatg aatctgcggg atcagcttga acggcgcatc
      541 cggacatcga tccttaacga cgaatgcaag gccttctaca agttccgttt ccaggccgcg
      601 gtttccaggt atcgcacgat gtcggtttat tgggatcgga ttctgcggga tcttgaaacc
      661 ggccgtacca gccgtgcgaa gctgcgggcc gaaaagggtt acggggcggc agccgtcgaa
      721 aatatcgaaa ctccgcggat gaggcgcatt cgcgaggccg cggcaaagaa cgcggcaggc
      781 gcggacggcg gtgacggcgg tgacggcggt gacgggacgg cgggcacgca gtccgggccg
      841 gcccacggtc gggcatctct ttacgaacag ttcatccagg cccgccaatc ggtcggactg
      901 ccgaccgagg ggattacgcc ggaaggattt gccaggacgc ttgcaaagca gaagcagatc
      961 cacaccgcca acccggaaat cagggacgtc gaattttccg tcaaggtgaa ggaaggtcgc
     1021 gtcgtcgttg ttgcccgccc agtcaaatag ccatttggtg gaacaattcc cgcggcaggg
     1081 gggtcttgat gcttttttcc cagcagattc gtttcaagcg tgacggccgt gcccttcaac
     1141 ctgaagcgat acgttcattc atccgtgaat acgcgtccga catgatcccc gattaccaga
     1201 tggccgccat gctgatggcc gtgtttttca aggggctcaa tcccgacgag ttgtgggcgt
     1261 ggacggatgc gatgcttcgt tccggcgacg tgatggactt ttcggatatc ccgggtgcca
     1321 aggtcgataa gcattcgaca ggcggggtcg gcgacaagat atcgattccg ctggccccgc
     1381 tggtggccgc atgcggcgtc aacgttccga tgatttcggg gcgcggcctg gggcacaccg
     1441 gtggaacgct ggacaagctc gaatcgatac ccgggttcaa gacgcggatc gggacaaacg
     1501 aatttcgcgc ggcgcttgcg gcaaacggcg tcgcgatgct tggccagacc gaaaacatcg
     1561 tgccggccga caagcgcatg tattcgctgc gggacgtcac cggaacggtc gagtcgattc
     1621 ccctgattgc ggcgtccatc atgtcgaaga aactggccga gggcatttcg ggcctggttc
     1681 ttgacgtgaa ggtgggcagc ggcgcgttca tgaagggcgt tgaccaggca cgtgaactgg
     1741 caggaacgct tgtcgcggtc ggccaggccg gtggggttcg cacgaccgcg ttcctgaccg
     1801 acatggacga gccgcttgga acgatggtcg gaaatgccct tgagattgtt gaatctgtgc
     1861 agatcctgcg caatgaaggc ccggccgata cgaccgcact gacggtcgaa cttggcgcca
     1921 ggatgctcct gcttggcggg gtggttgtca gtcacgagga aggtcgcgaa aggatcctcg
     1981 gcgcgattgc gtccggggcg gggctcgaca agttccgctc gatgattgcg gcgcagggtg
     2041 gaaatcctgc tgtcgcggac gacctttcga tccttccgcg ggccgcccgc cagatcggcg
     2101 tcaaggcttc acggaacggt tatgtgacgg ccgtcgattg cgaaaaggtc gggattgcgt
     2161 ccctgctggt cggcggcggc cggcgcagga aggaagacga aatcgatcct gccgtcggcg
     2221 tgcgcattct ggcccgaagg ggaactttcg tgtccgtggg cgacgacgtg gcggtcatcg
     2281 aatacaactc ggacgccagc ctggatgcgg cgtcgaagct tctgtcgtcg gccttttcga
     2341 ttggggatga aatggttccg gccggaccgc tggtcatcga cgtgatcgac ggcgccgccc
     2401 tttgaatgga gattgtcgga catgaagttt gaatactcga acccggttca ggtcgttttc
     2461 gggcccggca ggatttcgga actggggaag ttgacggcgg gcatcggtaa tcgcgcagtc
     2521 gtggtgattg gcggcggaag cgtccggaac aacgggaccc tggacaaggc cgtcaaatcc
     2581 ctggaagcgg cccgcgtcgc gatccatgtt tttgaaggtg tcgagccgaa tcccaggatt
     2641 tcgacggtgc gtcgcggaat cgacgcgatc agggcgtttc gcggcaacgt ggtcatcgcc
     2701 cttggcggcg gcagtgtgat ggacgcggcc aaggtgatgg cggccggcgc cctttacgac
     2761 caggatccct ggaagatgtg caaggtgtcc caggcgaagc ctgtggcccc aaccgcaagc
     2821 cttcccgtga ttacggtccc cacgctggcg gccaccggtt ccgagatgaa ctgcaatgcg
     2881 gtcatcacga actgggaagc gaacatgaag gcggccgcga tttatccgat cctttatccg
     2941 aagcttgcct taatcgatcc cgaactgacc gtcaccgttc cgaagtatca cacggccgcc
     3001 ggcgtggtcg atatcatcac ccacgtgacc gagggttatt tcaacgctca accggggacg
     3061 ccgttgcagg atcgcatggc cgaagccgtg atttcgacgg cgatcgagca tggcgcaagg
     3121 gcggtcgcgg acggttccga cctggcggcc cgcgaggcgg tcatgtgggc ttcgaccgtc
     3181 gcgttgaaca actggatcca cgtcggctgg aacgcgcctt tcccgtgcca cggcatcgaa
     3241 cacgtccttt ccgcgcatta tgacgtttca cacggcgccg gcctggccgc aatcagtcct
     3301 tcatggatgc ggttcgttgt ccgcagggtc gggccgggca aatgggccgg ctttgcccgt
     3361 cgcgtcatgg ctgtcgattg cgcagacgat gtcgaggccg cgctgctcgg catcgacagg
     3421 ctggaagagt tcttcgcgtc catcggggct ccgaccaggc tttcgggcct tggcatcccg
     3481 gccgcggaaa tccggcggct ggccgaggac accgtgcgcg tgttcggtgt taacggggcg
     3541 atcgcgggcg tgccgccgct gaccgtcgat gacatcgaaa gcatcctgaa ctccgccgcg
     3601 tgaccggtcc cggcgggcga
//
LOCUS       DAOPPA010000334         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27880_length_3618_cov_4.196183, whole genome shotgun sequence.
ACCESSION   DAOPPA010000334 DAOPPA010000000
VERSION     DAOPPA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27880_length_3618_cov_4.196183"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="eno"
                     /locus_tag="PKH54_10095"
     CDS             complement(<1..909)
                     /gene="eno"
                     /locus_tag="PKH54_10095"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD00289.1"
                     /translation="MTTIIDVFAREVLDSRGFPTVEVEVELECGAHGRAIVPSGASTG
                     AGEALELRDGDKARYMGKGTLKAVENVNDIIAPEVVGMDAEDQRELDMFMLELDGTPD
                     KTKLGANAILGVSVAAAHAAADANGQYLYRYIGGMSARTLPVPMLNVINGGQHADNNL
                     DIQEFMILPKGAPNFREGLRWASETFHTLKKILKGKGLNTAVGDEGGFAPNLGSNEEG
                     LAILVQAIEQAGYKPGEEISICLDVASSTFYSEETKLYTLGADGTKLTTPEMVDYLVG
                     LTKKFPITSIEDGLAEFDWEGFKMLTD"
     gene            1158..2462
                     /locus_tag="PKH54_10100"
     CDS             1158..2462
                     /locus_tag="PKH54_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00290.1"
                     /translation="MKQTVLLIAPSLGFESIFPLGLSTIASALMADGKVCIGLDERAR
                     PGCLDAALAGIDPDNRPMAAVIEASPRNVNQALDTAARCAAADIPAIFVGPAAATDAR
                     AMMDSSCAFAAVTGDPETVLRRLLAELANPAGAEIPGARFRTAAGVLTDSPPVFDMDL
                     AGLVYDRTVFPVSDYCDHPRHPEFRQAAIETSRGCTLDCTFCPVPCRFGRTWRGRPIA
                     AIVQEMSGLHRDYGITDFVIEDDQPLADIERFQTLMKAIRTALPGISMAFSNGLRPEL
                     LNISTLQLMAAAGTRDISLGIESGSSKVRASLNRPISTDQVQGIITEAHRLKMVVTGY
                     FMLGLPGETVPQMLETIMMANRFRFDYVHFMVYHPWHVTTGPASLAFNHVRTAAYLGS
                     YCNPMRLAGLARSGHISPGRARADASRLIEWIMAGRTGGGTW"
     gene            complement(2470..3204)
                     /locus_tag="PKH54_10105"
     CDS             complement(2470..3204)
                     /locus_tag="PKH54_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD00291.1"
                     /translation="MNAECRNASFVRKSPENPVRPVKAVIFDFDGTLVDSEPNYYVSD
                     RAMMLSYGIDLTEELKREFIGVGAVQMMQVLRQRFGIAAPVSELVRIKDEFYLRQSVG
                     NTVAYPQMWRLVRTLHAAGIPMAVASGSTPMVLDTLMVETGLRPYFSAVVSASEVPRS
                     KPFPDIFLEAARRLGVESADAAVFEDAVLGVRAGLAAGMRTCAIPGFPEDDLAPAYLD
                     ADLLFPGGMSFFSADVAMEWLGVPHP"
     gene            3407..>3618
                     /locus_tag="PKH54_10110"
     CDS             3407..>3618
                     /locus_tag="PKH54_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00292.1"
                     /translation="MNVFLRIVPIAAVAAIWTAPSTVTAEPDEGGLAEAPAWVSDCAA
                     ELKNRGETGLCGTGVTNSTPDSFFCES"
BASE COUNT          693 a         1132 c         1099 g          694 t
ORIGIN      
        1 atcggtcagc atcttgaagc cttcccagtc gaactcggcc aggccgtcct cgatggacgt
       61 gatcgggaac ttcttcgtca acccgaccag gtaatcgacc atctcggggg tggtcagctt
      121 cgtgccgtcc gcgcccaggg tgtaaagctt cgtttcttcg ctgtagaacg tgctggatgc
      181 gacgtccagg cagatgctga tttcttcacc gggcttatag ccggcctgct cgatcgcctg
      241 caccaggatg gccaggcctt cttcgttgct gcccaggttc ggggcgaagc cgccctcgtc
      301 gccgacggcc gtgttcagac ccttgccctt caggatcttc ttcagggtgt ggaacgtctc
      361 ggaggcccag cgcaggcctt cgcggaagtt cggggcgccc ttcggcagga tcatgaattc
      421 ctggatgtcc aggttgttgt cggcgtgctg gccgccgttg atgacgttaa gcatcggcac
      481 cggcagggtg cgggccgaca tgccgccgat gtagcggtac aggtactggc cgttggcatc
      541 ggctgccgcg tgggcggcgg cgacggacac gccgaggatg gcgttggcgc ccagcttcgt
      601 cttgtcgggg gttccatcca gttccagcat gaacatgtcc agttcgcgct gatcctcggc
      661 gtccatgccg acaacttccg gggcgatgat gtcgttgacg ttctcaacgg ccttcagagt
      721 acccttgccc atgtaccggg ccttgtcgcc gtcgcgcagt tccagggcct cgccggcacc
      781 ggtggacgcg ccggacggga cgatggcgcg gccgtgggcg ccgcattcaa gttcgacttc
      841 gacttcgacg gtcgggaaac cacgcgaatc cagaacttca cgagcaaaga catcaataat
      901 cgtggtcatt gggtaactcc ttgatttctg taaaaataac aagcatggcg gtggtttgac
      961 ccgcccttga accgccggaa tcataaaccc gtgggcccgg tggcgcaaga ccgagcgggg
     1021 ttacaccgtg aatattttca caaacgtagg ccattcaggc gcgaattacg caaccatcaa
     1081 ctattctgct aggatttgaa acgatgtcgc gtccgagcag cccggcccgt ccgagccggc
     1141 tcaacacggg gttcccgatg aagcagacgg tccttctgat cgcgccaagc cttggattcg
     1201 aaagcatctt cccgcttggg ctttcgacca tcgcatcggc cctgatggct gacgggaaag
     1261 tctgcatcgg gcttgacgaa cgtgcacggc ccggctgtct tgacgccgcg cttgcgggaa
     1321 tcgacccgga caaccggccg atggccgccg tcatcgaggc atccccccgc aacgtgaacc
     1381 aggcccttga caccgcggcc aggtgcgccg ccgccgacat tcctgcaatt ttcgtcgggc
     1441 cggccgccgc aaccgacgcg cgtgcgatga tggattccag ttgcgcgttc gcggcggtca
     1501 ccggcgaccc cgaaacggtc ctgcgacggc tgcttgccga gctggcaaac cctgctggcg
     1561 ccgaaattcc cggggcgcga ttccggactg cggcaggcgt cctgaccgat tcacccccgg
     1621 tctttgacat ggacctggcg ggacttgtct atgaccgcac cgtttttccg gtctccgatt
     1681 actgcgacca tcccaggcac ccggaattca ggcaggccgc catcgaaacg tcccggggat
     1741 gcacgctcga ctgcaccttc tgtcccgtcc catgcagatt cggccgaacg tggcgcggcc
     1801 gtccgatcgc ggcgatcgtg caggaaatgt cgggactgca tcgggattac ggaatcaccg
     1861 acttcgtaat cgaggacgac cagccgcttg ccgacatcga acgcttccag acactgatga
     1921 aggcaatcag gactgcactt cccggcatca gcatggcctt ttccaacggc ctgcggcccg
     1981 aactactgaa catttcgacc cttcaactga tggccgccgc cggaacccgc gacatctcgc
     2041 ttgggattga gtcgggaagc agcaaggttc gggcgtcgct gaaccgcccg atctcgacgg
     2101 accaggtgca gggcatcatc accgaagccc acaggcttaa gatggtcgtg accggatact
     2161 tcatgcttgg cctgcccggc gagaccgtcc cgcagatgct tgaaacgata atgatggcga
     2221 accgtttccg gtttgattac gtgcacttca tggtttatca cccgtggcac gtgacgaccg
     2281 ggccggcaag ccttgcgttc aatcacgtac ggaccgccgc ataccttggc tcgtactgca
     2341 acccgatgcg ccttgccggg ctggcccgtt caggccacat cagcccgggc cgggcccgcg
     2401 ccgatgcatc ccgcctgatc gaatggatca tggccggtcg aacgggcggc ggcacgtggt
     2461 aaggtcgatt caggggtgcg gcacgccaag ccattccatt gcgacgtcgg cggaaaagaa
     2521 ggacattcca ccgggaaaca gcaagtcggc atccaggtat gccggcgcca ggtcgtcttc
     2581 cgggaagccg gggatggcgc aggtgcgcat tccggcggcc aggccggcgc ggacgccaag
     2641 gacggcatcc tcgaaaactg cggcgtccgc ggattcaacg cccaggcggc gggcagcttc
     2701 cagaaagatg tccggaaacg gcttcgaccg ggggacttcg gatgcggaca cgacggcgga
     2761 gaaatatggc cgcaacccgg tttcaaccat caaagtgtca agaaccatcg gggtcgaacc
     2821 gcttgcgacc gccatgggga tcccggccgc atgcagggtg cgcaccaggc gccacatctg
     2881 gggataggcc acggtgtttc caacggactg gcgcagatag aattcatcct tgatgcgcac
     2941 cagttcggaa accggggcgg caatgccgaa cctttgtcga agcacctgca tcatctgcac
     3001 ggcgccgacc ccgatgaatt cccgcttcag ttcttccgtc agatcgattc cgtatgaaag
     3061 catcatggcg cggtcggaaa cgtaataatt gggctcgctg tcaaccagcg tgccgtcgaa
     3121 atcaaaaatt accgccttga cgggacggac tgggttttcc ggggatttcc tgacaaagct
     3181 cgcgttccga cattctgcat tcatctgatg gcatccatag tgctggcaaa agaccgacgg
     3241 cccgcatcgg gcggaaccga cgcaatgata cagccattgc gcccgattcc gcgcacccgg
     3301 ctgcggaacc atataaaaac cccttgattt gacgcgatat tcaatcggtt ctaacatagt
     3361 tgaatcggtt catttgggaa cacgacactt cgacaggagg tgtgccatga acgtgtttct
     3421 tcgtattgtc ccgattgcgg cggttgcggc gatctggacg gctccatcca cggtgaccgc
     3481 cgaacctgac gaaggcggtc tggccgaggc acccgcctgg gtcagcgact gcgccgccga
     3541 gcttaagaac cgcggcgaaa cgggcctgtg cgggaccggg gtcacgaaca gcacgcccga
     3601 cagtttcttc tgcgagtc
//
LOCUS       DAOPPA010000335         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27942_length_3612_cov_4.240090, whole genome shotgun sequence.
ACCESSION   DAOPPA010000335 DAOPPA010000000
VERSION     DAOPPA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27942_length_3612_cov_4.240090"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKH54_10115"
     CDS             complement(<1..573)
                     /locus_tag="PKH54_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOD00293.1"
                     /translation="MQTFVVSALHVLLASSLSVHILLNKKSPARAGIWIILVWFIPVA
                     GALLYWGFGDNRIARRAKRRLKAKRPDGTPDSSGRVPSNLIPLQVAGDRIAHNPLVPG
                     NHIEVLENGDQAYPAMLAAIASARKSVAFLTYIFDSDEVCRHFTDVLCDAARRGVQVR
                     LLVDGIGARGFGPVLRRRLSEAGGRMASFWR"
     gene            complement(624..2576)
                     /locus_tag="PKH54_10120"
     CDS             complement(624..2576)
                     /locus_tag="PKH54_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOD00294.1"
                     /translation="MGKEARGICPHCQQQTGDAGTPCPNPECGRLGYYLIPILSFDAS
                     KARAEKKGAGIEPIIGRRIDRYLMVGKAGEGGMGAVYIALQQPLNREVAVKIMPSIHL
                     SPEALERFETEAKAVSALDHPNIVKLYDYGFGEIEGQRVPYMVLEYIKHGRTMKQLMT
                     QEFAEHEVLRSELTLQIFEQVLGGLGAAHAVGIVHRDVKPENVMLTQIHGNPYYAKIL
                     DFGLATGLHGNSTGMTGSNQILGTPTYMAPEQIPSSRRQEIDLRTDLYAVGVMLFECM
                     TGSRPFPGDTALAVLTAKSNEKFNPFDAPVAARLTKPQRAFLEKAISFDSDGRFQSAG
                     EMLAALNEAIAQSGARKRGGLLGFMEDKLGTQLPGLGSRGPATPEKTNPPAGITGSGA
                     TPNPITGMAMHSMSGMAQNPLAGMPPGSLSGMAQNPLAVQGIPLNGGPTPSTSPGIRR
                     PARTTTNPIPAVNVTPGWQSQTSEIRRTGQSSMTPEERAERLRGRKRRTTGTHMARAV
                     EAERAAAGTHDNRGLPTMLVVFGIIVAIIAVSVAVPRYLESRRIDRTQADLKDLRSSL
                     VIAGKFVGLDSPVLRAGTQLDYFISDPGRPESPLLDAWGNPYRVRYSLQNPAGYVLYS
                     TGSDGEKGTCDRAPGNDDICVELGLP"
     gene            2822..3046
                     /gene="rpmE"
                     /locus_tag="PKH54_10125"
     CDS             2822..3046
                     /gene="rpmE"
                     /locus_tag="PKH54_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD00295.1"
                     /translation="MKKEIHPKYPEAKISCACGNVIETHSTRGSYAVEICSSCHPFFT
                     GKQKIVDTEGRVDRFNRKYAGLNPLSQIRK"
     gene            3043..>3612
                     /locus_tag="PKH54_10130"
     CDS             3043..>3612
                     /locus_tag="PKH54_10130"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOD00296.1"
                     /translation="MTMVSKTPSVGGQAVVEGVMMRAPDATSVAVRRPDGSIVVRVRS
                     QASLSKRFPALARPGLRGMATLLETMADGISALNFSAVHAMPQEQAGEGDKPGAAMTT
                     GAIVGTLVVSMAFGFALFAALPHFLTWGIGMLMGSDALAGGKAAAFHLVDGFIKLCIF
                     VLYIWLVSFMKDMRRVFEYHGAEHQAIFAF"
BASE COUNT          665 a         1096 c         1088 g          763 t
ORIGIN      
        1 cctccagaaa ctggccatcc tgccgccggc ctccgacagt ctccgcctga gaacgggccc
       61 gaagcccctc gcgccgatgc cgtcaaccag aagcctgacc tgcaccccac gtcttgccgc
      121 gtcgcagagc acgtccgtga agtggcggca cacttcgtcg gaatcaaaaa tgtaagtaag
      181 gaatgccacg cttttcctgg cgcttgcaat cgccgcgagc atcgccggat aagcctggtc
      241 accgttttca agaacctcga tatggtttcc gggaacaagc gggttgtgtg caatgcgatc
      301 gccggcgacc tgaagaggta tcaggttcga gggcaccctt cccgaactgt cgggcgtgcc
      361 gtcgggcctt ttggccttca ggcggcgttt ggcccgccgc gcgatgcggt tgtcgccgaa
      421 accccagtag agcagggcgc ccgcgacagg gatgaaccat acgaggatga tccagatgcc
      481 tgcgcgcgcg ggacttttct tgttaagaag aatatgaacc gaaagtgagg atgccagcag
      541 gacatgcagc gccgaaacaa cgaacgtttg cataggtcca ccagaagaaa cgccgtcggc
      601 ccgggaagcc ccggcgcaaa aggtcatggc aagccaagtt ccacacagat gtcgtcgttg
      661 ccgggggcac ggtcgcaggt gcccttttcg ccgtcggaac cggttgaata gaggacatat
      721 ccggccgggt tctgcagcga gtaccgaacg cggtaaggat tgccccaggc gtcaagcagc
      781 gggctctccg gccggccggg atcgctgatg aaatagtcga gctgggtgcc cgcccggagc
      841 accgggctgt caagcccgac gaacttgccg gctatcacca gcgaagaccg cagatcctta
      901 aggtcggcct gcgtcctgtc gattcggcgg ctctccaggt atctcggcac ggccacagag
      961 acggcgatta tggctacgat gatgccgaat accacgagca tcgtcggcaa tccccgattg
     1021 tcatgagtcc cggccgccgc cctttcggcc tcgaccgccc ttgccatgtg ggttccggtt
     1081 gtccgccgct tccttccacg aagtctttcc gcacgttcct ccggggtcat cgagctttgc
     1141 ccggtgcgtc tgatctcact ggtctggctt tgccaccccg gagtcacgtt gaccgcgggg
     1201 atggggttgg tcgtcgtcct ggccggtctg cgtatccccg ggcttgtcga tggcgtcgga
     1261 ccgccgttaa gcggtattcc ctgaactgcg agcgggttct gcgccatccc ggacaaggat
     1321 cccggcggca tcccggccag ggggttctgc gccatcccgg acatcgaatg catggccatg
     1381 ccggtaatcg ggttcggagt cgccccggaa ccggtgattc cggctggcgg attcgtcttc
     1441 tcaggtgtgg ccggacccct cgacccgagc ccgggaagct gagtgccgag tttgtcttcc
     1501 atgaaaccga gcaggccgcc acgcttcctt gcacccgact gggcgatggc ctcgttcagg
     1561 gcggccagca tctcgccggc gctctggaag cgaccatccg agtcgaagct gatggccttc
     1621 tcaaggaacg ccctctgcgg cttcgtaagc cttgccgcca cgggggcatc gaaaggattg
     1681 aacttttcgt tcgatttggc cgtcagcacc gccagcgccg tgtcgcccgg gaatggcctg
     1741 cttcccgtca tgcattcgaa cagcatgacg ccgaccgcgt agaggtcggt cctgagatcg
     1801 atttcctggc gcctgctgga cgggatctgc tcgggggcca tgtaggtcgg cgtgcccaga
     1861 atctggtttg aaccggtcat tccggtgctg ttgccatgaa gtccggttgc cagcccgaag
     1921 tcgaggatct tcgcgtaata cggattgccg tgaatctgtg tcagcatgac gttctcgggc
     1981 ttaacgtccc gatgcactat gccgacggcg tgcgcggcgc ccaggccgcc aagaacctgt
     2041 tcaaaaatct gcagcgtcag ttcgctgcgc aggacttcgt gttccgcgaa ttcctgtgtc
     2101 atgagctgtt tcatcgtccg gccgtgcttg atgtattcaa gcaccatgta aggaacgcgc
     2161 tggccctcta tttccccgaa accgtagtcg taaagcttga cgatattcgg gtgatccagt
     2221 gcagagaccg ccttggcctc ggtctcgaag cgttcgaggg cctcggggga aaggtggatc
     2281 gacggcatga tcttgacggc gacctcgcgg ttcagcggct gttgaagcgc gatatacacc
     2341 gcgcccatgc cgccctcgcc ggccttgccg accatcaggt agcgatcgat cctgcggccg
     2401 atgatgggtt cgattcccgc gcccttcttt tccgcgcggg ccttggatgc gtcgaatgaa
     2461 agtatcggaa taaggtaata gcccagccgt ccgcattcgg gattagggca cggcgttccc
     2521 gcgtcgcccg tctgttgctg gcagtgtggg catatgcccc gagcttcctt ccccattgcg
     2581 ttcaaaataa agtgcccgat agaatatata gtgaacgtgc ggttacggtc aatctaatgg
     2641 gtgaatattt acgcgcccgg acaagatttc ctgttcatat tgtcccgccc tggtccacaa
     2701 gcgcttgcct tcggcaataa ccaaggctcc tttcgcggat tttcgttgac tcaatcactg
     2761 aaaatggata ccattccgcg ccccgatcag gttttgatcg agtaaccgtc tggaggacga
     2821 tatgaagaag gaaattcatc ccaaatatcc ggaagccaag atttcgtgcg catgcggcaa
     2881 cgtaatcgag acccattcga cccgcggttc gtacgccgtt gaaatctgtt cgtcgtgcca
     2941 cccgttcttt accggcaagc agaagatcgt cgataccgaa ggccgcgtcg atcggttcaa
     3001 ccgcaagtac gcaggcctga atccgctcag ccagatccgc aaatgacgat ggtatcaaag
     3061 acgccttccg ttggcggaca ggcggtcgtt gaaggcgtca tgatgagggc gccggacgcg
     3121 acctcggtcg ctgtaaggcg gccggatggt tccatagtcg tccgggtgcg ttcccaggct
     3181 tcgctttcaa agcgatttcc tgcgcttgcc aggcccggtc ttcgtggcat ggcaaccttg
     3241 ctcgagacga tggccgacgg tatctccgcc ctgaatttct cggcagttca cgcgatgccc
     3301 caggagcagg cgggcgaagg cgacaagccg ggcgcggcga tgaccacggg cgccatagtc
     3361 ggaacccttg tcgtttccat ggcatttggt ttcgcgctct ttgccgcgtt gccccatttt
     3421 ctcacctggg gaatcggcat gctgatgggc agcgatgcgc tggccggagg aaaggccgcc
     3481 gcttttcatc tggttgacgg tttcataaag ctctgcatat tcgtcctcta tatctggctg
     3541 gtgtccttca tgaaggacat gcggcgcgtc ttcgagtacc acggggcgga gcaccaggcg
     3601 atattcgcct tc
//
LOCUS       DAOPPA010000336         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_27945_length_3612_cov_3.505763, whole genome shotgun sequence.
ACCESSION   DAOPPA010000336 DAOPPA010000000
VERSION     DAOPPA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27945_length_3612_cov_3.505763"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..2723
                     /locus_tag="PKH54_10135"
     CDS             264..2723
                     /locus_tag="PKH54_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOD00297.1"
                     /translation="MRAVAECCRTDRGRDQITRLAPIARVEDARAELALVDEAAVLLV
                     HDGIDCLEPVPAMDIEIAQARKGGVLANDELVAFARTLAVAGRVRKHILDARLRAPGL
                     AVIFEGTKSVDAVCERIEQTFNEDGTIRDDASPLLQELRSKVRTISRRVKGTIEEMLK
                     DGQVQQVLQDDYFTLREGRYVLPVKSEDYRFIPGIIHGTSQTGHTFFVEPSRIVEDNN
                     QLKIAVDEIESEENAIRADRTRLIARFSLEIVELCDALWRFDSIVGRAAFMRRYGGDT
                     VKAPEIVTGRASMVLKSAVNPVLALQRERPLKVVPIDLDVSLDGHDAEAGYAVIISGP
                     NAGGKSVTLSTVGLSCMLVRHGVVPLVGEGTRIPWYETILTVIGDPTDMDKAVSNFTG
                     QLVRVDEILKSRAGRTLVLIDELATGTEPSRGEALATAIVEHLVDAGDECLVATHYDL
                     LKRTAIGDRRFVNVRVGVEKATGQPTYRIEHGEIGESNPFEVARSIGFPERIVKRALE
                     MTGIRERQLEEALQKAQALNAELLAERTATADLRATLAEDKRKYSAELVRLRKESDRL
                     VYQARQDVLRKMKDLEEELEKIGKEARAERAEAAKREQMIIRRRDEVRVKKEAVQKDM
                     QAEAELVGDIPGTKLDAAELASGMKVYVVKLRSEGTIVDVSAGGRKVTVQVGLLKTSV
                     KPEDLRKPEPKKAGGGNVPGGLTGKQRDRLAKESGAPTRNPIVELQAADIERMFVRSP
                     ENTIDVRGFRVDDALAAVDKFLDTAFVGEEPQVMIIHGMGTSALRKAIRDAIARHPQV
                     KKSRSGDRDEGGDGVTIIDMA"
     gene            complement(2727..>3612)
                     /locus_tag="PKH54_10140"
     CDS             complement(2727..>3612)
                     /locus_tag="PKH54_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOD00298.1"
                     /translation="PLTPMCPLRAAWRTETIRPLAETPLFKENSPAIDEGFLMDSSST
                     ATTVTAAEKLTLGRKLKAAPWYKWLSVTLRHLYDWVLSWASSPYGWLALFLIAFAESS
                     FFPIPPDVLLIALVLGWRKKAFHLAALCTLGSVLGAMFGFWIGSAFFGPVNSLMEVIL
                     GHDTWFGVAGQAATAVQLDGFTFFRYADGAHGQSVFLTVKDLYDKNAFMALFTAAFTP
                     IPFKVFTIAGGYFQIPFGILVIASAIGRAMRFFLVAGLLFIFGEPMKRFIEKYFDILA
                     LLFMVLLIGGFVLIGKVL"
BASE COUNT          819 a          956 c         1210 g          627 t
ORIGIN      
        1 agggcgaaga ccatggtcaa ggagcataac agtccggtcg tcgcgaatac gttacgatac
       61 ttcatcgggc ctccaaggga taaaagggcg gatcatccta ccaaccgcga ccattccatg
      121 tcaaaccaag acgaaggcat gggggtaccg cgaaaccggg gattggttca caatacgtgt
      181 caggattgac ccattacctt gaaggaaccg ctttgaacca gatgaatgca cggacagtca
      241 gagatttgca gtttgaacga atcatgcggg cggtggcgga gtgctgccga accgaccgcg
      301 ggcgtgacca gataacgcgg ctggcgccaa tcgcccgtgt cgaggatgcc cgggctgaac
      361 tggccctggt cgatgaggcc gcggtgctgc tggtccacga cggcattgac tgtctggagc
      421 cggtgccggc aatggatatc gagatcgccc aggcccgcaa gggcggggtg ctcgccaacg
      481 atgaacttgt cgcgttcgcc cgcacgctgg cggttgccgg tcgcgtgcgc aagcacatcc
      541 tggatgccag gctgcgcgcc ccggggcttg ccgttatctt cgagggcacg aagagcgtcg
      601 atgccgtttg cgagcggatt gagcagactt tcaatgaaga cggcaccatc cgcgacgatg
      661 ccagccccct gctgcaggaa ctgaggtcga aggtcaggac tatcagccgc agggtcaagg
      721 gcaccatcga ggaaatgctg aaggacggcc aggttcagca ggtgttgcag gatgactact
      781 tcacgctgcg cgagggtcga tacgtcctgc ccgtcaaatc ggaggactac cggttcattc
      841 caggcatcat tcatggaacc agccagaccg ggcatacgtt cttcgtcgag ccgtcgcgca
      901 tcgtcgagga caacaaccag ctcaagattg ccgttgacga gatcgaaagc gaggaaaacg
      961 cgattcgggc cgacagaacg cggctgattg cgcgcttcag cctggaaatt gttgaacttt
     1021 gcgacgccct ttggcggttc gattcgatcg tgggccgggc cgctttcatg cggcgatacg
     1081 gcggcgatac ggtcaaggcc ccggagattg ttaccggccg ggccagcatg gtactcaagt
     1141 cggccgtaaa cccggtgttg gccttgcaga gggagcgccc ccttaaggtt gtgcccatag
     1201 atcttgacgt atctcttgac gggcatgatg cagaggccgg gtacgcggtt atcatcagtg
     1261 gcccgaacgc cggcggcaag tcggtgacgc tttcgacggt cggcctgtcg tgcatgttgg
     1321 tgcgacatgg ggtggtgccg cttgtgggcg aaggcacgcg gattccctgg tatgaaacga
     1381 tcctgaccgt catcggcgat ccaaccgaca tggacaaggc cgtttcgaat tttaccgggc
     1441 agcttgtgcg cgttgacgag atcctgaagt cgcgcgcggg gcgcacgctg gttcttatcg
     1501 acgaattggc gaccggtacc gagccatctc gaggcgaggc actggcgacg gcgattgtgg
     1561 agcatctggt cgatgccggc gacgaatgcc ttgtcgcgac ccattacgac ctgttgaagc
     1621 gcacggcgat tggcgatcgg cgattcgtca atgtacgggt cggtgtcgaa aaggccacgg
     1681 ggcagccaac ctatcgtatc gaacacggtg aaatcggcga aagcaacccg ttcgaggtgg
     1741 cccggtcgat tgggttcccc gaaagaatcg tcaagcgggc cctggaaatg acggggattc
     1801 gcgagcgcca gcttgaggaa gcgttgcaga aggcccaggc gctgaacgcg gaactgctgg
     1861 ccgagcggac ggcgactgcc gatttgaggg cgactcttgc cgaggacaaa cggaagtatt
     1921 cagccgaact ggtaaggttg cgcaaggaaa gcgaccggct ggtttatcag gcaaggcagg
     1981 acgtgctgcg caagatgaag gatctggaag aagaacttga aaagattggg aaagaggcca
     2041 gggccgaaag ggccgaagcg gccaagaggg aacagatgat aatcaggcga agggacgagg
     2101 ttcgcgtgaa gaaggaagcg gtccagaagg atatgcaggc cgaggccgaa cttgtcgggg
     2161 atatcccggg gacaaagctg gacgccgcgg aactcgcgtc cgggatgaag gtctatgtgg
     2221 tgaagctgcg atcggaagga accatcgtcg atgtttcggc gggcgggcga aaagtcaccg
     2281 tccaggttgg tcttttgaaa acatcggtga agccggaaga cctgcgcaag cccgagccga
     2341 agaaggctgg aggcgggaat gtgcctggtg ggctgactgg aaagcagagg gaccggctgg
     2401 cgaaggaatc cggtgccccg accaggaatc caatcgttga gcttcaggcg gccgatatcg
     2461 aaaggatgtt cgtgagatcg ccggaaaaca cgattgacgt gcgcggattc cgggtggatg
     2521 acgccctggc cgcggttgac aagttccttg acactgcttt cgtgggcgag gagccccagg
     2581 tgatgattat tcacgggatg gggacgtcgg cactgcgaaa ggcgattcgt gacgcgattg
     2641 cgcgtcatcc acaggtcaaa aagtccagaa gcggcgaccg cgacgaaggc ggcgatggcg
     2701 tgacgatcat cgatatggcc taggcgtcag agcacctttc caatcagcac gaaaccaccg
     2761 atcagcagca ccatgaagag cagggcaagg atgtcgaagt acttctcgat gaagcgcttc
     2821 atcggttcgc cgaagatgaa aagcaggccg gcgaccagga agaaccgcat ggcgcgaccg
     2881 atggccgagg cgatcacaag gattccgaac ggaatctgga aatagccgcc ggcgatcgtg
     2941 aagaccttga acggaatggg cgtgaaggcc gcggtgaaaa gggccatgaa agcgttcttg
     3001 tcgtaaagat ccttgaccgt caggaaaacg gactggccat gggcgccgtc ggcgtaacgg
     3061 aagaacgtga atccgtcgag ctgcaccgcc gttgccgcct gacccgcaac accgaaccat
     3121 gtgtcgtgcc caagtatgac ttccatcagg ctgttgaccg ggccgaagaa ggccgagccg
     3181 atccagaagc cgaacatcgc gccgaggacg gaacccaggg tgcatagggc ggccaggtgg
     3241 aacgcctttt tccgccagcc cagaaccagg gcgatcagca ggacgtcggg cggaatcggg
     3301 aagaacgacg attccgcaaa ggcaatcagg aacagcgcaa gccatccgta cgggctgctg
     3361 gcccacgaga gaacccagtc gtacaggtgg cgcagggtga ccgaaagcca cttgtaccag
     3421 ggcgcggcct tcagcttgcg gcccagcgtc agcttttcag ccgcggttac tgtcgttgcg
     3481 gtactgcttg aatccatcag gaaaccttca tcaatggcgg gtgaattttc cttgaaaagc
     3541 ggcgtttcgg caagcgggcg tatcgtttca gtgcgccatg ctgcccggag ggggcacatc
     3601 ggtgtcagtg ga
//
LOCUS       DAOPPA010000337         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28016_length_3604_cov_4.032967, whole genome shotgun sequence.
ACCESSION   DAOPPA010000337 DAOPPA010000000
VERSION     DAOPPA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28016_length_3604_cov_4.032967"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..601)
                     /locus_tag="PKH54_10145"
     CDS             complement(89..601)
                     /locus_tag="PKH54_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD00299.1"
                     /translation="MAVKYDAVVVGAGVAGLSFATRMAGYGYRVVVLESANPEHLGHD
                     WWDTIELRSFDVLGLARPEPPEARTPFTFKMNVLGRTYPVVARMPDDMVNVERRLLAT
                     RMVRWATQADVEIRFGCHVTGPILENGRCKGVTLATAGDHAARVKAPLTIDASGAAAV
                     LRRFIENVAA"
     gene            complement(681..2042)
                     /locus_tag="PKH54_10150"
     CDS             complement(681..2042)
                     /locus_tag="PKH54_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOD00300.1"
                     /translation="MQKKAPVINHIWFWLIVIPILAAIATGRMEDVTKAGFESAALAV
                     ELALKLIGVMTLWLGLTKIAEAGGLMTMIANGLKPVMRRIFPSIPEGHPALGAMVMNF
                     SANMLGLGNAATPLGIKAMMELNRLNRTPGQATNAMILFLAINTSSIAILPLGVIGVR
                     AAAGASNPADILVPGLLATICSTTVAIAVGLLINRLRPDPAADKTGHDGSADAPATAS
                     AGGGGATIPAADDPTTVTAPNEASATTTVPDSLINRLVPILVVLLALAAIVWRLVNGD
                     MSGMPGEIVKWWMIPLIMVAILLFGYRRGVNVYEAACDGGREGFQTAVRIIPYLVMIL
                     VAIGIFRASGAFEMVTSALDPLTSLVGLPSEVLPIAILRPLTGSGSFALMSEITANAP
                     NGFPAYLAGIMQGSTETTFYVLAVYFGSVGITKIRHALFPALLADLTGIAAAIIFARV
                     FFG"
     gene            2175..>3604
                     /locus_tag="PKH54_10155"
     CDS             2175..>3604
                     /locus_tag="PKH54_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD00301.1"
                     /translation="MKMKFAGCCILGCLFLAVGCGDGSSSGNDLGPDVVGDTVVGDTV
                     AGDTVTPDVPVDTAVETTEPIPIDWEAFLHPEGAGFPQDGKKRVMILHTNDIHSHVNG
                     TGPVLDYTPETIMDDDTIGGFARIASLIERQKAWIDENVGMAIMDAGDFSFGTAFTAL
                     APSTGLELRMMDEMGYVGTTIGNHELDWTPAGLVEVLNQGLEGATNISVLASNLVYSD
                     ESADDDAMAALVGDKIKPYKVVTLENGVKIGMFGLLGVNAIKLSPNAEPVTARALKET
                     ALEMVDTLRNVEGVDLVIALSHSGVSEGTAKGEDEALAEQVDGIDIIVSGHTHTYMED
                     PIIVKDTVIVQSACYGKFLGRLVMVQDGQGGWDLESWEPIPVTDETPGWPAMIETVQD
                     YMDMLGDTAFASLGLGYADPIATTDFDLVPVEFKEYSLGNLIADGVRYSAKKFSGRDF
                     DVAFEANGVIRDSLLKGKSGRITLGD"
BASE COUNT          733 a         1026 c         1137 g          708 t
ORIGIN      
        1 gataaaaatc aggcggcctt gatttttcaa gaccgcctga cgggctgttt attccgttta
       61 tgtttttggg gtatgcggag aatccggatc aggccgcgac gttttcaata aagcgccgca
      121 ggaccgcggc ggcaccgctt gcgtcgatgg tcagcggtgc cttcaccctg gcggcatggt
      181 cgccggccgt ggccagtgtt acccctttgc agcgtccgtt ttccaggatc ggtcccgtga
      241 cgtggcaccc gaaccggatc tcgacatcgg cctgggtcgc ccatcgaacc atgcgcgtgg
      301 caagcagcct tcgctcgaca ttgaccatgt cgtccggcat gcgggcgacc acggggtaag
      361 tccgcccaag aacgttcatt ttgaatgtga atggcgttct ggcttcgggc ggttcgggcc
      421 ttgccagacc caggacgtca aaggatcgca gttcgatcgt gtcccaccag tcgtggccaa
      481 ggtgttccgg attcgcggat tccagtacta ccacccgata cccgtaacca gccatcctgg
      541 tcgcgaagga aagaccggcc acgccggccc cgacaacaac tgcgtcatat tttactgcca
      601 tgaatcaccg gcctggagtc gtgtggatga taccggcttt tgtgcggtgc gtcacgaaat
      661 cggacgccct gtggccgtgg tcagccgaag aagacgcggg caaagatgat tgcggcggcg
      721 attccggtaa ggtcggccag cagggcgggg aacagcgcgt gcctgatctt cgttatgccg
      781 accgatccga agtacacggc gagcacataa aacgtcgttt cggtgctgcc ctgcatgatg
      841 ccggcaaggt acgccgggaa tccgttcggg gcgttcgcgg tgatttcgga catcagcgcg
      901 aacgatccgg agccggtcag ggggcgcagg atggcgatgg gaagaacctc ggatggaaga
      961 ccgacaaggc tggtcagcgg atcaagagcc gacgtgacca tttcgaacgc tccggacgcc
     1021 ctgaagatgc cgatcgcgac caggatcatt accagatatg gaatgattcg aacggcggtc
     1081 tggaatcctt cacgcccgcc gtcgcaggcg gcctcataaa cgttgacgcc gcgcctgtag
     1141 ccgaaaagaa ggatcgcgac catgatcagg ggaatcatcc accatttcac gatttcgcct
     1201 ggcatgcccg acatgtcgcc gttgacgagg cgccagacga tggcggcaag cgcaaggagt
     1261 acgaccagga tcggaaccag tctgttaatc agggaatcgg gcaccgtcgt ggtcgccgac
     1321 gcttcgttcg gggcggtgac ggtggtcgga tcgtcagccg ccggaatcgt cgccccgccg
     1381 ccacctgccg acgcggttgc cggcgcgtcc gccgaaccgt cgtgcccggt cttgtcggca
     1441 gccggatccg ggcgaaggcg gttgatcaga aggccgacag caatggcaac ggtcgtggaa
     1501 cagatggtcg caagcaggcc cggcaccagt atgtctgccg ggttggatgc tccggcggca
     1561 gcccgaaccc cgatcacccc aagcgggagg atggcgatgc tgctggtatt gatggccagg
     1621 aaaagtatca tcgcgtttgt cgcctgaccc ggtgtgcgat tcaggcggtt cagttccatc
     1681 atcgccttga tccccagtgg agtcgccgcg ttacccaggc cgagcatgtt ggccgagaag
     1741 ttcatcacca tggcgccaag cgcggggtgt ccttcgggaa tcgaggggaa aatccgtcgc
     1801 atcacgggct tcagaccatt ggcgatcatc gtcatcaacc cgccggcttc ggcaatcttc
     1861 gtcagcccga gccacagcgt catgacgccg ataagcttca gggcaagttc gacggccagg
     1921 gccgcggatt cgaagccggc cttggtcacg tcttccatgc gtccggtcgc gatcgcggca
     1981 agaatcggga tgacaatcag ccagaaccag atgtgattga tgacaggagc ctttttctgc
     2041 atcccggcgc ctcgcaagcg atcgtccaaa gataaccgat gaagacgatt tccgataagc
     2101 cggtgcagga agcggtgccg tttgtcgaag cgttgtgata tcattccgcc attctggttg
     2161 aaggaatccc tgccatgaaa atgaagtttg ccgggtgctg cattcttggt tgcctgttcc
     2221 ttgccgtggg ttgcggcgac ggttcgtcga gcggaaatga tctcgggcct gacgttgtcg
     2281 gcgatacggt cgtgggcgat acggttgcag gcgatacggt cacgcccgat gtccctgtcg
     2341 atactgccgt cgagacgacc gaaccgattc cgatcgactg ggaagccttc cttcatcccg
     2401 aaggcgccgg gttcccgcag gacggcaaga aaagggtaat gatcctgcac accaacgata
     2461 tccattcgca tgtgaacggt accggcccgg ttctggacta cacgccggaa acgatcatgg
     2521 acgacgacac gatcggcggg ttcgccagga tcgcctcgct gatcgagcgc cagaaggcgt
     2581 ggattgatga aaacgtcggc atggccatca tggacgccgg cgatttcagt ttcggaaccg
     2641 ctttcacggc gcttgcccct tcgaccgggc tggaactgcg aatgatggac gaaatgggat
     2701 acgtcggaac gacgattgga aaccacgaat tggactggac cccggcaggg ctggtggaag
     2761 tcctgaatca gggccttgaa ggcgccacga acatttctgt tcttgcttcg aacctggtct
     2821 attcggacga atcggccgat gacgacgcca tggctgccct tgtcggggac aagatcaagc
     2881 cgtacaaggt cgtgacgctg gaaaacggcg tgaagattgg gatgttcggg ctgctgggcg
     2941 tcaacgccat caagctgtcg cccaacgcgg aaccggtaac ggcgcgcgcc ctgaaggaaa
     3001 ccgcgctcga gatggtcgat acgcttcgga atgtcgaggg tgtcgatctg gtcatcgcgt
     3061 tgtcgcacag cggtgtatcg gaaggaaccg ccaagggcga ggacgaggcc cttgccgagc
     3121 aggttgacgg catcgacatc atcgtcagcg gccacaccca cacctacatg gaagatccga
     3181 taatcgttaa ggatacggtc atcgtccagt ccgcctgtta cggaaagttc ctgggcaggc
     3241 ttgtgatggt tcaggacggc cagggcgggt gggatcttga atcatgggag ccaatccccg
     3301 tgaccgatga aactccgggt tggcccgcca tgattgaaac cgtccaggat tacatggaca
     3361 tgcttggcga caccgctttt gcttcccttg gcctcggtta tgcggatccg attgccacga
     3421 ccgatttcga cctggttccg gtcgagttca aggaatacag ccttggaaac ctgattgccg
     3481 acggcgtgcg ttattcggcg aagaagtttt ccggacgcga tttcgacgtg gcgttcgagg
     3541 cgaacggcgt catccgcgac agcctgttga agggcaaatc cggccggatc acgcttggcg
     3601 acat
//
LOCUS       DAOPPA010000338         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28048_length_3600_cov_4.148096, whole genome shotgun sequence.
ACCESSION   DAOPPA010000338 DAOPPA010000000
VERSION     DAOPPA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28048_length_3600_cov_4.148096"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="PKH54_10160"
     CDS             complement(<1..2331)
                     /locus_tag="PKH54_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00302.1"
                     /translation="MMKKFPLSVLVVTLIAFAGCGDGGGRSDLSGDESFIPDVPQDIE
                     PDHSGTDVPTDETADDADGADYGQDVAGNDSIEPVDVVDAGDTPLDAPMELPEPDAAF
                     VWEIRNRQGCVADGPWKVDRVLRLGAGTAGLGDVDVETAVDVAGRVWLVSDGRLLVAD
                     QADPAAAGFSDVTAELAGAPAMAIQASGDVLLVATGAGIESFDSARVHSVVSSTPMEY
                     ARILQCDGEIWAVAAGRLYLIQEAPFEVVLEEGAGEVVSLACLDGDVVVGATGGVFSG
                     PGGDMLELRWEPGDDAIVDVAAGNGIVVAVTATGLAVIDAASWQSTLVEPGIGGLPAG
                     GMHQAAVSADGRRVAIAHEVGCTLLDLLPGRGDALAVNRIDVGDGKPAVVDHFNSARW
                     LPSDTVNSVAFGPDGETLWVSTAAGISRIRPQQLVLADKAAEMMDNLDRWFWRLEGFV
                     TAAAGFESATSDVRLQLQDNDNDGQWTQEAVGAFCYAYRVTGDERYYQAARKAITNMC
                     MLIDVPAQDFIDAGLGRGFVTRSFVRDDEGALFTEKAALSNWHLVDYMDGHQYYWKDD
                     TSSDEMTGHFYGFSIYHDLCAKDDTERAWVASHLTDLVSYIVTHGYRLLDLDGEPTEH
                     GKYGPEYLAVAVDGLEACTLTYDMETCIGAWGGGAFLDSTEILGGLLAAWHVSGDRRF
                     YDAYESLISEHRYDELATFNEFVMTWTNPQIANYCDHELSDLALLTLIRYEQRPDRRA
                     IWIKSMLDAWQYEVGERNPLKTLTMAAALADIEGIDG"
     gene            2516..>3600
                     /gene="prs"
                     /locus_tag="PKH54_10165"
     CDS             2516..>3600
                     /gene="prs"
                     /locus_tag="PKH54_10165"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOD00303.1"
                     /translation="MAHKDFLLFAGNSHLGFARAIARQLGVVLHEPDESDRRGPTIKW
                     FSNGNVLVDPKINVRGKHCFVIQTQAPGRALIGKDSNGNDVFGVNLTVSDMIIELYWM
                     VHALTCAGARVTVVTPYLPYIRSDKQDHPRASIGARTFADLLTSVGARGIVIMEPHFQ
                     QIHGFFDQKAIKVDTLNMKPIFAYEILANSERDKTVFVAPDIGEAKHLGPFVNMLKMD
                     IAIINKERTSDNESARAQHMVGNVEDRDCWLIDDETMSGGTLVEAADFCVTKGARSLR
                     AAICHPVLTSMDGLKRIEAHPLIKELLVTDTIPVPPEKRISKLRVRSVAPEFARAIEI
                     LAALPGEDNSLDAFKESLYSPINDLLKR"
BASE COUNT          763 a         1155 c         1046 g          636 t
ORIGIN      
        1 gccgtcgatc ccctcgatat cggcaagcgc ggcggccatg gtcagcgtct tcaaaggatt
       61 gcgctcgccg acttcgtatt gccaggcgtc cagcatcgac ttgatccaga tggcccggcg
      121 gtccggccgc tgctcgtacc gaatcagcgt cagaagagcc aggtcggaca gttcgtggtc
      181 gcagtaattg gcaatctgcg gatttgtcca ggtcatcaca aattcgttga atgtcgccag
      241 ttcgtcatag cggtgctcgg aaatcagcga ttcgtacgcg tcataaaagc gcctgtcgcc
      301 cgaaacgtgc caggcggcaa gcaacccgcc aagtatttcc gtcgaatcaa ggaaggctcc
      361 gccaccccac gcgccgatac aggtttccat gtcgtacgtg agcgtgcacg cctccagccc
      421 gtcaacggcg acggcaaggt attccgggcc gtacttgccg tgctcggtcg gctcgccgtc
      481 aaggtcgagc agcctgtaac cgtgcgtcac gatgtaggaa accaggtcgg taaggtggct
      541 ggcgacccag gcacgctcgg tatcgtcctt cgcgcaaagg tcgtggtaaa ttgaaaaacc
      601 gtaaaaatgc ccggtcattt cgtcggaaga cgtgtcgtcc ttccagtagt actggtggcc
      661 gtccatgtaa tcgaccaggt gccagttcga aagcgccgcc ttttccgtga acagagctcc
      721 ttcgtcatca cggacgaacg acctggtcac gaaaccgcgc cccaggccgg catcgatgaa
      781 atcctgggcc ggaacgtcaa tgagcatgca catattggtg attgccttgc gcgccgcctg
      841 gtaataacgt tcgtcaccgg tcacgcggta cgcatagcag aaggcgccga cggcttcctg
      901 ggtccactgc ccgtcgttgt cgttgtcctg cagttgcagc ctgacgtccg atgtcgcgct
      961 ctcgaagcct gccgccgcgg ttacgaaacc ttccaggcgc cagaaccagc ggtccaggtt
     1021 gtccatcatc tcggccgcct tgtcggcaag caccaactgc tgcggacgga tgcgcgaaat
     1081 accggccgcg gtcgaaaccc acaatgtttc accgtccggc ccgaacgcga cggaattgac
     1141 cgtgtccgac ggaagccacc tggccgaatt gaagtgatcg accacggccg gctttccatc
     1201 gccgacgtca atccggttga ccgcaagcgc atcgccccgt cctggcagca aatccagcag
     1261 ggtgcatcca acctcgtggg caatcgcgac acgccgaccg tcggcggaaa cggccgcctg
     1321 gtgcatgccg ccggccggca gtccgccaat gcccggttca acaagagtgg attgccagct
     1381 tgcggcatcg atcacggcga gtccggtggc ggtgacggcc acgacaatgc cgttgccggc
     1441 cgccacatcg acgattgcgt cgtcgcccgg ttcccaccgc aattcaagca tgtcgccgcc
     1501 ggggcccgaa aacacgcctc ccgtggcacc gacaacgaca tcgccgtcaa ggcatgccag
     1561 ggacaccact tcgccggcgc cttcttcaag aacgacttca aacggcgctt cctggatcag
     1621 atagagccgc cctgccgcga ccgcccagat ttcgccatcg cactgaagga ttcgggcgta
     1681 ctccatcggg gtggatgaca caacagagtg cacgcgggcc gaatcgaacg actcgattcc
     1741 agcgccggtc gccaccagaa ggacatcgcc gcttgcctga atcgccatcg cgggggctcc
     1801 ggcaagctcg gcggtcacgt ccgaaaatcc ggcggccgcc ggatcggcct gatccgcgac
     1861 aagaaggcga ccgtcggaaa ccagccagac ccgccccgcg acgtccacgg ccgtttccac
     1921 atcgacatcg ccaagcccgg ccgtgccggc gccaagacgc aggacgcgat caaccttcca
     1981 gggaccgtcg gcgacgcacc cctgccgatt gcggatctcc cacacgaagg cggcatccgg
     2041 ctcgggcaat tccatcggcg catcaagagg cgtatcgccc gcatcaacca catcaaccgg
     2101 ttcgatcgaa tcgttcccgg cgacgtcctg cccgtaatcc gcaccgtcgg cgtcatcggc
     2161 cgtttcgtcg gtcggaacgt ccgtgccgct gtgatccggc tcgatatcct gggggacgtc
     2221 cggaatgaac gattcatctc cagacagatc ggatcggccc ccgccgtcgc cgcagcctgc
     2281 aaaggcaata agagtcacga ccagaaccga cagtggaaat ttcttcatca tgaaaacctc
     2341 gctgaggcat caatagtacc gcaacaagat tcgatttcca cggaaaagcc ggccgccggg
     2401 aagcgccgcg gggcggccat gagggttgat aattcggctc caaagtgtta atcggcaaaa
     2461 ccgaaacaga accgctttct ggttccatcc agatcggcaa acagaggtca cgaaaatggc
     2521 acacaaggat ttccttcttt tcgcggggaa ttcgcatctt gggtttgcac gggcgattgc
     2581 caggcaactg ggcgtcgtgc tgcacgaacc ggacgaatcg gatcgacgcg gcccgaccat
     2641 caagtggttt tcgaacggca acgtcctggt cgatccgaag atcaacgtcc gcggaaagca
     2701 ctgcttcgtg atccagacgc aggcgcccgg ccgggctttg atcggaaagg attcgaacgg
     2761 gaacgacgtg tttggcgtca acctgacggt cagcgacatg atcatcgagc tgtactggat
     2821 ggttcacgcg ctgacatgcg ccggtgcccg cgtcacggtg gtgacgccgt acctgcctta
     2881 catccgcagc gacaagcagg atcatccccg ggcaagcatc ggggccagga cgttcgcgga
     2941 tctgctgaca agcgtcggcg cccgcggcat cgtcatcatg gaaccgcatt tccagcagat
     3001 tcacgggttc ttcgaccaga aggccatcaa ggtcgatacg ctgaacatga aaccgatctt
     3061 cgcgtacgaa atccttgcga attcggaacg cgacaaaacg gtcttcgtcg cgcccgacat
     3121 cggcgaggcg aagcacctgg gcccgttcgt gaacatgctg aagatggata tcgccatcat
     3181 caacaaggaa cgcacgtccg acaatgaatc ggcaagggcc cagcacatgg tcggcaacgt
     3241 cgaggatcgc gactgctggc tgatcgacga tgaaacgatg tccggcggca cgcttgtcga
     3301 ggcggccgat ttctgtgtca cgaagggcgc ccgatcgctg cgcgcggcca tctgtcaccc
     3361 cgtcctgact tcgatggacg gtttgaaaag aatcgaggcg catcccctga tcaaggaact
     3421 cctggtcact gacacaattc ccgttccgcc ggaaaagcgt ataagcaagc tgcgcgtcag
     3481 gtcggtcgcg ccggaattcg cccgggcaat cgaaatcctt gccgccctgc cgggcgagga
     3541 caactcgctg gacgctttca aggaaagcct gtacagcccg atcaacgacc ttctgaagcg
//
LOCUS       DAOPPA010000339         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28050_length_3600_cov_3.877292, whole genome shotgun sequence.
ACCESSION   DAOPPA010000339 DAOPPA010000000
VERSION     DAOPPA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28050_length_3600_cov_3.877292"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2769)
                     /locus_tag="PKH54_10170"
     CDS             complement(<1..2769)
                     /locus_tag="PKH54_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOD00304.1"
                     /translation="MRIFKIFAGLSMVALTLAGRPAYAVDPLFDPPPPVVHILLDTSG
                     SMQYDSQGSTNEDYWDLADDAVPVCSPDPGVSGKSRYIVATEVLCGTLQDYWCEYESR
                     GIGSSHCPNADCYWSVPHVKAHGTRMNDGLLDRVGASFKFGVMTMDSNPLPFTNSSGG
                     YSYGPEWLIDLGAQNENSPIGRLVPPSASDDPADITTTNARVRQSILDARPWSVTVLG
                     PMLYDAEYLIRNHPVHTADPYDACREKHIILITDGRNNTGLDIFTYLDPVAQANRLRR
                     LGFEVWVVGFNMAPGYSEELWEIATYNGALPDDHLLYATDGIDLANALRQIFSNVARS
                     TQSRTRPVVTQNTGNYTDVQYQFNAGYAVAMDATGGNYIPDVMQGILERSVYKCGVTS
                     LVPGTAGLGSIQSIGDKLSSTGDGLRNIYTMVAGDLESFENGTSDITNEMLAAPNPAA
                     GPVPDFGRDTVSGWCKSAHLSGTNEEKLAQWRLNLFNYVRATADSCRASYKLGAIYHS
                     TPALHDNLRDVNLPIPSFTNYKQSISTRPGMLYVATHDGMLHAFRTDRPDARYEPDTW
                     GKELWAYIPAHQLPYLKLLPEGRETLLDGSPVVADLILSRTATSRLTEDEDSWKSVLL
                     FGDRGGGRGYSALDVTDPTNGNWSVLWEISAANGRCPGGSITCNPGGASEYENDFQKL
                     GYTFGRPAIGTVMICPDMSGTCAQTRLEEVGVAVFGGGSSEGIAGAGIGRTLYVVRLD
                     TGEKLAEFRTGNSDGDDSDVVSSCDYLQGNIDADIVGDITCISTMPGTFLTRCWVGDS
                     AGRLWRVELGSPDRSEWRMSLLYDPYDFTGGAPAVNAAIRAPAYEAPAVSTTRGTNEP
                     VVIYTGGDMDNLRTVNQASFVVSLTDSIVDVIPADCSPWLDNACWRQIDGGLYSGPKV
                     NWKHFIGYD"
     gene            complement(2778..3395)
                     /locus_tag="PKH54_10175"
     CDS             complement(2778..3395)
                     /locus_tag="PKH54_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00305.1"
                     /translation="MIIHRSVCRRGRPKCHAARASRNGIGHQGGFILLLSLMLLLALT
                     ALGLITARYTASNMSFTGAQRVTSTAESIAVSGVESTLVMGAQNAGSFIAYLDVNGQA
                     VAMSDFAPNFFDAAVDGSGSFGRETGNINTASWRSRMVYSQLSTRAPGYQVGEHCFIR
                     YTSITDGFYQNQPNFADPDDIERNRERNALARDMATIFVGPILCP"
     gene            complement(3415..>3600)
                     /locus_tag="PKH54_10180"
     CDS             complement(3415..>3600)
                     /locus_tag="PKH54_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00306.1"
                     /translation="VTVKLTTRTVHEDEKFQMIQRQALFTPIEAYDLDPIMEGAARTV
                     SLSSRVGLKTFLVRNVK"
BASE COUNT          679 a         1066 c         1092 g          763 t
ORIGIN      
        1 gtcgtatccg atgaagtgct tccagttcac tttgggaccg gagtaaagtc cgccatcaat
       61 ctggcgccag caagcgttgt cgagccatgg cgaacaatcg gcggggatca cgtcaacgat
      121 gctgtccgtc agggacacga cgaagctggc ctggttgacc gttcgaaggt tgtccatgtc
      181 gccgccggtg tatatcacaa ccggttcgtt cgtcccccgc gtcgttgaaa ccgcgggtgc
      241 ttcatatgcc ggcgccctta tggcggcatt caccgccggc gcgcctccgg tgaagtcgta
      301 cgggtcgtaa agaaggctca tgcgccactc ggatctgtcg ggcgatccaa gttcgacgcg
      361 ccacaggcgc ccggccgagt cgcccaccca gcatcttgtc aggaaggtgc cgggcattgt
      421 cgagatgcac gtgatgtcgc ccacgatgtc ggcatcgatg ttcccctgaa ggtaatcgca
      481 actgctgacg acgtccgaat cgtcgccgtc ggagtttccg gttctgaact ccgcgagttt
      541 ttccccggtg tccagtcgaa cgacgtacaa cgtccttcca atgccggccc cggcgatgcc
      601 ctcggaactg ccgccgccga acaccgcgac cccgacttcc tcgagcctgg tctgggcgca
      661 ggttcccgac atgtcggggc atatcatcac agtgccgatg gccggcctgc cgaaggtgta
      721 tccgagcttc tggaagtcgt tctcgtattc ggaagctccg ccgggattgc aggtgatcga
      781 gccacccgga cacctgccgt ttgccgcgga gatctcccac agaaccgacc agttgccgtt
      841 tgtcgggtcg gttacgtcaa gcgcggaata tccgcgtccg ccccctcgat ccccgaagag
      901 cagaaccgat ttccaactgt cctcatcctc ggtcaacctg gacgtggccg ttctcgaaag
      961 gatcagatcc gccacgacgg gcgacccgtc aagaagcgtc tcccgtccct cgggcagcaa
     1021 cttcaggtaa ggaagctggt gggccggaat ataggcccag agttcctttc cccatgtgtc
     1081 cggttcatac cgcgcgtccg gcctgtcggt tctgaacgcg tgaagcatcc cgtcgtgggt
     1141 cgcgacgtaa agcatgccgg gtcttgtgga aatcgactgc ttgtagtttg taaacgatgg
     1201 aatcggcagg ttcacgtccc gcaggttgtc gtgcagggcc ggcgttgagt gatagatggc
     1261 gcccagtttg tacgacgccc tgcacgaatc ggcggtcgcc cggacgtagt tgaaaaggtt
     1321 caggcgccac tgggccagct tttcctcatt cgtgcccgag aggtgtgccg atttgcacca
     1381 gcctgaaacg gtgtcccggc cgaagtcggg gacggggccg gcggccgggt ttggggccgc
     1441 cagcatttcg ttcgtgatgt cgctcgtgcc gttttcaaat gattccaggt cgccggcgac
     1501 catggtgtag atatttcgga gtccgtcgcc ggttgaactg agcttgtcgc cgatgctctg
     1561 tatggatccg agtccggcgg tgcccggaac cagcgaggtc acgccgcatt tgtaaaccga
     1621 cctttcaagg atgccctgca tcacgtccgg gatgtagtta ccgcccgtcg cgtccatggc
     1681 gaccgcgtac ccggcgttga actggtattg aacgtcggtg tagttgccgg tgttctgggt
     1741 taccaccggc cgcgtccttg actgggttga tctggcgacg tttgaaaata tctgccgaag
     1801 cgcgttcgca aggtcgatgc catcggtcgc gtaaagcagg tggtcgtccg gaagcgctcc
     1861 gttgtaggtc gcaatttccc acaattcctc gctgtatccg ggggccatgt tgaatccgac
     1921 cacccacact tcgaacccca gccgccgcag cctgttggcc tgagcgaccg gatcaaggta
     1981 agtgaagatg tccagcccgg tgttgttccg gccatcggtt ataaggatga tgtgcttttc
     2041 acggcatgcg tcgtatgggt cggcggtgtg aacggggtga ttccggatca ggtattccgc
     2101 atcgtaaagc atcgggccaa gaacggtcac gctccatggg cgggcgtcaa gaatcgactg
     2161 tctgacacgg gcgttggtcg tcgtgatgtc ggccgggtcg tcggaagcgc ttggcggaac
     2221 caggcgaccg atcggggaat tctcgttttg cgcgccaagg tcgatcagcc actccggccc
     2281 gtaactgtaa ccgccgcttg aatttgtgaa cggcagcgga ttggaatcca tcgtcatcac
     2341 gccgaatttg aagctggcgc cgacccgatc gagaaggccg tcgttcatcc tggtaccgtg
     2401 tgccttgacg tgcggaacag accagtagca gtcggcgttc gggcagtgtg acgaaccgat
     2461 gccgcggctt tcgtattcgc accagtaatc ctgaagcgtg ccgcaaagta cttcggtggc
     2521 gactatgtaa cgactctttc ccgaaacccc gggatccggc gagcacaccg gaaccgcgtc
     2581 gtccgcaaga tcccagtaat cctcgttcgt cgagccctgg gagtcgtact gcatgcttcc
     2641 ggacgtgtcc agaaggatgt ggacgaccgg tggaggcggg tcgaaaaggg ggtcaaccgc
     2701 gtaggccggg cgaccggcca atgtcagtgc gaccatcgag agtccggcaa aaatcttgaa
     2761 aattctcatg gtgcacctca cgggcaaagg atcggaccga caaagatcgt tgccatgtcg
     2821 cgggccaggg cgtttcgttc acggtttctt tcgatatcgt cgggatcggc aaaattcggc
     2881 tggttctggt aaaagccgtc tgtaatcgaa gtgtatcgga tgaaacagtg ctcgcccacc
     2941 tggtacccgg gtgcccttgt cgaaagctgc gaatacacca tccggctgcg ccagcttgcg
     3001 gtgttgatgt tacccgtttc cctgccgaac gaaccggatc catcaacggc ggcgtcaaaa
     3061 aaattcgggg cgaaatcgga catcgccacc gcctggccgt tgacatcgag atacgcaatg
     3121 aaggatccgg cgttctgcgc gcccatgaca agagtggatt ccacgccgga taccgcgatt
     3181 gattcggccg tcgatgtgac cctctgggcg ccggtaaagg acatgttgga tgcggtgtac
     3241 cttgccgtta tcaagccaag tgccgtcagg gccagcagga gcatcaggga cagaagaagg
     3301 atgaagccgc cctggtggcc gatcccgttc cgcgaagcgc gcgccgcatg gcatttcggg
     3361 cgaccgcgcc ggcaaactga cctgtggatt atcatccgtc ccccttgacg cttcctactt
     3421 gacgtttcga accaggaacg tcttcagccc gacccttgat gacagcgaaa ccgtccttgc
     3481 cgcgccttcc atgatcggat ccaggtcgta cgcctcgatc ggcgtaaaca gcgcctgtcg
     3541 ttgaatcatc tggaactttt cgtcctcatg aacggttctt gtcgtcagtt tgaccgtcac
//
LOCUS       DAOPPA010000340         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28248_length_3581_cov_3.565230, whole genome shotgun sequence.
ACCESSION   DAOPPA010000340 DAOPPA010000000
VERSION     DAOPPA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28248_length_3581_cov_3.565230"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKH54_10185"
     CDS             <1..825
                     /locus_tag="PKH54_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOD00307.1"
                     /translation="RGRWLRHPGINLRNHRKILVIDGETGFTGGINISARHVTQPGAP
                     YPESFDLHFKLKGPVVGHLMETFTDDWELSTNETLTGPDWFPDLTPAGNVLARGIPSG
                     PDFSVGRLHDLLLAALRTARVSIDLMSPYFIPDEAIMASLRTSARSGVRVRVFIPSKA
                     DHRFMSWGARAYLRELIVCGVEVWEMGPSFNHSKLTVIDGEWLMMGSANLDPRSFRLN
                     FEFNVEAYSRELAARVADYMDRQVAGARRINMNVLDKESAFIRVRNRFIALFSPFL"
     gene            complement(833..2317)
                     /locus_tag="PKH54_10190"
     CDS             complement(833..2317)
                     /locus_tag="PKH54_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00308.1"
                     /translation="MKRIKEILGEHRAPLLLFVASAVVLGLFSWNWILSPSPHFHFVD
                     LAQSFLHGRLDTDTPRQHRGQKQRPEDPAGYRKAIDRHLTGANGSTTGWNDWASVREI
                     TMTDGTVVRGVFPWGNQEGSMKNRFHALDGTIRIIDPQLDIAKGCGQPSAKCDRKDWY
                     VSFPPFPAVVMIPLTAIFGYNVNDVLFTVLNGALNCMLLFLLLEFLVRQGLSSRTRRE
                     NLVLALLFCFGTVHFFSSIRGQVWFTAQILGVAINIWYISCAIGNRRPFLAGLAIACG
                     MATRTTIAFAAVFFALELFRDGDRLRWPGFGHLLRKGLPFAAPIVVGGILLMLMNYFR
                     FDNPMEFGHTFLADGTRASIRDHGLFSMWFARANLGAMLTNPPVIDGVAPFVHITRHG
                     LGLLWTSPFLLLVLWPRVWGAFQRNLAITIAVIAIPLICYQNTGWSQFSFRFALDFLP
                     FAMVLLAAGGRQLSGWFMAAGAFSVLMNTFGAVTFDRAGMFYYD"
     gene            2512..3267
                     /locus_tag="PKH54_10195"
     CDS             2512..3267
                     /locus_tag="PKH54_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD00309.1"
                     /translation="MMSVLSIAGLKKTYPNGNEAIRDISFEVGRGEFVFLSGPSGSGK
                     TTLIRILLKMEESTGGQVLVFGKNLNALLPSAVPYLRRNIGVIFQDFRLLQDRTVFEN
                     MALGLNILGLTTREISRRVEGILYDLGIDRYAGAYPAMLSGGEQQRVAIGRALVMEPA
                     LILADEPTGNLDWELSQDIVRLFEELNARGTSVLMATHDRFLLEAFPKRTLLINRGIL
                     VEDRNPGMAATRREAGQNRFQHFVAGRTDGSPA"
     gene            3302..>3581
                     /locus_tag="PKH54_10200"
     CDS             3302..>3581
                     /locus_tag="PKH54_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00310.1"
                     /translation="MTEEDTRADGATRLMRLVGQGFKGLVVAPWQTLACVVSLGVAAC
                     LIVLSASAGSLTMSMLGRASRAARILVYLKDDVESERILNNCLSQIVVE"
BASE COUNT          783 a         1041 c         1097 g          660 t
ORIGIN      
        1 cgggggcgat ggctgcgcca ccctggcata aaccttcgaa accacagaaa aatcctggta
       61 attgacggtg aaaccggatt caccggcggc atcaacatca gcgcaaggca cgtcacgcaa
      121 cctggcgcac cctaccccga atcattcgat ctccacttca agctgaaggg gcctgtcgtc
      181 ggtcacctga tggaaacctt taccgacgac tgggaactca gcaccaacga aacgcttacc
      241 ggcccggact ggtttccgga tctgacgccg gccggcaacg tcctggcccg gggcattcca
      301 tccggccccg acttcagcgt cggcaggctt cacgaccttc tcctggccgc gctcaggacg
      361 gccagggtct cgatcgacct gatgtcgcct tacttcatcc cggatgaagc catcatggcg
      421 tcgctgcgaa cgtccgcaag atccggcgtc agggtcaggg ttttcatacc gagcaaggcg
      481 gatcaccgct tcatgtcctg gggtgccagg gcttatctgc gcgagttgat cgtctgcggt
      541 gtcgaggtct gggagatggg gccttccttt aaccacagca agttgacggt catcgacggc
      601 gagtggctga tgatgggatc ggccaacctc gatccgcgaa gcttcaggct gaatttcgag
      661 ttcaacgtcg aggcatattc ccgtgaactg gcggcaaggg tcgccgacta catggacagg
      721 caggttgccg gcgcaaggcg gatcaacatg aacgtgctgg ataaggagtc cgcgttcata
      781 agggttcgca acaggttcat cgcgctgttt tcgccattcc tgtgatttcc gactagtcgt
      841 aatagaacat gccggcgcga tcgaatgtga cggccccgaa ggtattcatc agcaccgaga
      901 acgcccccgc cgccatgaac caaccggaaa gctggcgtcc gcctgccgca agaagcacca
      961 tcgcgaacgg aaggaaatcg agggcgaagc ggaagctgaa ctgggaccat ccggtattct
     1021 gatagcagat aagcggaatc gcgatgaccg caatcgtaat cgcaaggttc ctctggaaag
     1081 cgccccagac cctgggccaa aggacaagca gcagaaatgg cgatgtccac agaagaccaa
     1141 gcccatgcct ggttatgtgg acaaacgggg caacgccatc gatgaccggc gggttcgtca
     1201 gcatcgcgcc aaggttcgcc ctggcgaacc acatcgaaaa gaggccgtgg tcgcgaatcg
     1261 atgcccgggt accgtccgca aggaaggtat ggccgaactc catcgggttg tcgaacctga
     1321 agtagttcat cagcatcagc aggatcccgc cgacgacgat cggggccgcg aatggcagtc
     1381 ccttgcgaag aaggtggccg aatcctggcc atcgaagcct gtcgccgtca cggaaaagtt
     1441 ccagcgcgaa gaaaacggcg gcaaaagcaa tcgtggttct ggtggccatc ccgcaggcga
     1501 ttgccagccc cgcaagaaac ggcctgcggt tgccgatggc gcaactgatg taccagatgt
     1561 tgatggcaac accgaggatc tgcgcggtga accagacctg accccggatc gaactgaaaa
     1621 agtgaaccgt gccgaaacag aaaagcagcg ccagaacaag gttttcgcgc ctggtgcggg
     1681 aagatagtcc ctgccggacc aggaattcca ggagcaggaa gagcagcatg cagttcaggg
     1741 cgccgttaag aacggtgaac aggacgtcgt tcacgttgta cccgaagatg gccgtaagcg
     1801 gaatcatcac gacggccgga aacggtggaa aagacacgta ccagtccttc ctgtcgcact
     1861 ttgccgaagg ctgcccgcac cccttcgcga tatcgagctg cgggtcgatg attcgaatcg
     1921 ttccgtccag ggcgtgaaac ctgttcttca tcgatccttc ctggttgccc cagggaaaga
     1981 caccgcggac aacggttccg tccgtcatcg tgatttcccg gacggatgcc cagtcgttcc
     2041 agccggtcgt ggagccgttc gcgccggtca ggtgacgatc gatggccttg cggtaaccgg
     2101 ccggatcctc cggacgctgc ttctggcccc tgtgctgcct gggggtatcc gtatcaagcc
     2161 ggccgtgaag gaatgactga gccagatcga caaagtgaaa gtggggcgac ggcgacagga
     2221 tccagttcca ggaaaacagt cccaggacaa cggccgatgc gacgaaaagc agcaacggcg
     2281 ccctgtgttc acccagtatt tccttgattc ttttcaaaat cggctccaac aggtttcggg
     2341 gctgaaaccc cggacatgga ctcgtccgtc aacccgattc ggccgcttca ggccacccgg
     2401 attccgaacc tgccgaaata taccagacgc cggcgccttc tgctattcta cgacggtcgg
     2461 aaacgggcaa ggcgccctgg tcggcgtttc cggcgatgct gcctggagtc catgatgtcg
     2521 gtcttatcga ttgcgggcct gaaaaagact tacccgaacg gcaacgaggc catcagggac
     2581 atctcgttcg aggttggcag gggcgaattc gttttccttt cgggcccaag cggctccggg
     2641 aagacgacgc tgatccggat cctgctgaag atggaggaaa gcaccggggg ccaggtgctg
     2701 gtgttcggaa agaaccttaa cgccctgctg ccctccgcgg tgccctatct gcgccgcaat
     2761 atcggcgtca tcttccagga cttcaggctg ctgcaggatc ggaccgtatt cgaaaacatg
     2821 gcacttggac tgaatatact ggggctgacg acacgcgaaa tttcaaggcg ggtcgagggc
     2881 atactgtatg accttggcat cgatcggtac gccggagcct atccggccat gctctccggc
     2941 ggtgaacagc agcgtgtggc aatcggccgg gcactggtca tggaacccgc gctgatactg
     3001 gctgacgaac cgacaggcaa ccttgactgg gaactgtcgc aggacatagt ccggcttttc
     3061 gaggaattga acgcccgtgg aacgtccgtc ctgatggcga cacacgacag gttcctgctt
     3121 gaggctttcc cgaagaggac gctccttatc aaccggggaa tccttgtcga ggatcgcaac
     3181 cccgggatgg cggccactcg gcgggaagcg ggacagaacc ggttccagca tttcgtggcg
     3241 ggccggacgg atggcagtcc cgcctgaccg gtgacagcgc cggtcgtgac ttggtgtgtt
     3301 gatgactgaa gaagatacaa gggcagacgg ggccaccagg ctaatgaggc tggtcggtca
     3361 gggcttcaag gggctggtgg tcgccccatg gcagaccctt gcctgcgtgg tatcccttgg
     3421 ggtcgcggcc tgcctgatcg tcctgtcggc ttccgccggc agcctcacga tgtcgatgct
     3481 gggccgggca tcgagggccg ccaggatact ggtttacctg aaggacgatg tcgaaagcga
     3541 acggattctt aacaactgcc tcagccaaat cgtagtggaa g
//
LOCUS       DAOPPA010000341         3579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28265_length_3579_cov_4.313848, whole genome shotgun sequence.
ACCESSION   DAOPPA010000341 DAOPPA010000000
VERSION     DAOPPA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28265_length_3579_cov_4.313848"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /gene="gyrB"
                     /locus_tag="PKH54_10205"
     CDS             <1..2323
                     /gene="gyrB"
                     /locus_tag="PKH54_10205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOD00311.1"
                     /translation="GSGLHHMVYEVVDNSIDEALGGYCNHIKVFIEPGNIVSVEDNGR
                     GIPVGIHKETGRSAAEVVMTELHAGGKFDHENYKVSGGLHGVGVSVVNALSEWLRVDI
                     RREGGAFTQTYRRGIPDGDLVRIGDSDETGTKVTFKADSKVFSDTNFQFDTLSQRLRI
                     LAYLNPGVRITFGDLRDEGEPKSADYCFEGGIVSFVEHLNRHKTCLHDKPIFISGARE
                     GVSVEVSMQWTNGYQETLYCFTNTIMNRDGGTHVAGLRGALTRTINNYAESEGLLKQF
                     KGTLSGEDVREGLTVVLSTKVHDPKFSSQTKDKLVSSEVKGAVEAIVNEALADFFEMN
                     PAVARGVVGKTIEAARARDAARKAREMVRRKGVLDGASLPGKLADCQERDPANAELFI
                     VEGDSAGGSAKQGRNRANQAILPLRGKILNVEKARFDKMLESEAIVTLITALGTGIGP
                     DSFDIARLRYHRIVIMTDADVDGLHIRTLLLTFFFRHMPEVIERGYLYIAQPPLYKVR
                     KGKTEMYLMDDGELESFLLENGIAGVSVLAAGDMFPLSEDDLKRYLSRISIARRLAGQ
                     IDMRWDSRVFKALFDEGDFTMADVDKPGSDSIVGNMIATWMEKVGSPQEPVTVERRVQ
                     EDGTASLLVVTRRNGGAVRTWLDHKTFEQHEVLQFRKVMNDLRKVAHWPYHVTLGERD
                     MQVTMPDELLTLIMDRGQKGMSYQRYKGLGEMNAGQLWETTMDPARRTLLKVNIVDAI
                     KADEIFTLLMGDAVEPRREFINENALNVINLDV"
     gene            2348..3034
                     /locus_tag="PKH54_10210"
     CDS             2348..3034
                     /locus_tag="PKH54_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00312.1"
                     /translation="MTSYLEDVESLFLSAVRKGLSLRVSDLEIVRDWDRRRVPIDVVS
                     RGILYGIQRFLAQAEPDAPVPASLKYYRTAVEKEFQTWERAAARGLAMTSAVPQAARK
                     AGPDLPLTERARNILLQRISDATGATRDVYAEALNHYDAAVKSTPLADLLFQVEDMVV
                     AGLGKNLGDERLAAIAAASAGQAAAAAARGVGPRALRDLERNLFRKAVAAELGFSSFL
                     DEVLDRPGRK"
     gene            3053..>3579
                     /locus_tag="PKH54_10215"
     CDS             3053..>3579
                     /locus_tag="PKH54_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00313.1"
                     /translation="MKCARCKDLGYVTAPLGEFAVARVCECRTPCPECNGTGLIIDAS
                     TLPRKAAVCSCRHLNRRVELFNRAGLPARMHSKTLDLFEERSPAQARTKTFIRRFTED
                     YAPGNRGFLLWGKPGTGKTHLVCSMSRHFLLEMGWSVKFVDFFQLLSDLKAGFSDGRS
                     EAELISPLVAPDVLI"
BASE COUNT          759 a         1061 c         1108 g          651 t
ORIGIN      
        1 cggatccggc ctgcatcaca tggtgtatga agtcgtcgac aactcgattg acgaggcgct
       61 cggcggttac tgcaaccata tcaaggtctt catcgaaccg ggaaatatcg tttcagtcga
      121 ggacaacggc cgcggaatcc ccgtcggaat ccacaaggaa accgggcggt cggccgcgga
      181 agtcgtcatg accgaactgc acgccggcgg taagttcgac cacgaaaact acaaggtttc
      241 cggtggcctc cacggcgtcg gcgtatcggt cgtcaacgcc ctttccgaat ggctccgggt
      301 tgatatcagg cgggaaggcg gcgcattcac ccagacttac aggcgcggca ttcctgacgg
      361 cgacctcgtc aggatcggcg acagcgacga gacgggcacg aaagtcacgt tcaaggccga
      421 ttcaaaagtt ttctctgaca cgaacttcca gttcgacact ctcagccagc ggctgcgtat
      481 ccttgcatac ctcaacccgg gtgtccggat caccttcggc gacctgcgcg acgagggcga
      541 accaaagtcg gccgactact gtttcgaggg cggcatcgtc tcgttcgtcg agcacctgaa
      601 ccggcacaag acctgccttc acgacaagcc catattcatc agcggggcac gtgagggtgt
      661 cagcgtcgaa gtttcgatgc agtggaccaa tgggtatcag gaaacccttt actgtttcac
      721 caacacgatc atgaaccgtg acggtggaac ccatgtggcg ggcctgcgcg gcgcgctgac
      781 aagaaccatc aacaactatg ccgaatccga gggactcctg aagcagttca agggcaccct
      841 gtccggcgag gacgtccggg aaggcctgac cgtcgtgctt tcgacaaagg ttcatgaccc
      901 gaaattctca tcccagacca aggacaagct ggtttcgtcc gaagtcaagg gtgccgtcga
      961 agcgatagtg aacgaggcgc ttgcggactt ctttgaaatg aacccggcgg tagcccgcgg
     1021 agtcgtcgga aagacgatcg aggccgcgcg cgcccgtgac gcggccagga aggccaggga
     1081 aatggttcgc cgcaagggcg tcctggacgg cgccagcctt ccaggcaaac ttgccgactg
     1141 ccaggagcgc gacccggcca acgccgaact gttcatcgtc gagggcgaca gcgcaggcgg
     1201 ttccgccaag cagggaagga acagggccaa ccaggccatc ctgccgcttc gcggaaagat
     1261 cctcaacgtt gaaaaggccc gtttcgacaa gatgcttgaa agcgaggcca tcgtcacgct
     1321 gatcaccgcg ctcgggacgg gaatcggccc agacagcttc gacatcgcca ggctcaggta
     1381 tcaccgcatc gttatcatga ccgatgccga cgttgacggc cttcacatcc ggacgctgct
     1441 tctgactttc ttcttccgtc acatgccgga agtaatcgag cggggctacc tgtacatcgc
     1501 ccagccaccg ctttacaagg ttcgcaaggg caagaccgag atgtacctga tggacgatgg
     1561 cgaactggaa tccttccttc tggaaaacgg aatcgccggc gtgtcggttc tggcggccgg
     1621 cgacatgttc ccactgtcgg aagatgacct gaagcgttac ctgtcccgca tatcgattgc
     1681 caggcgcctt gccggccaga ttgacatgcg ctgggacagc cgcgtgttca aggctctgtt
     1741 cgacgaaggc gatttcacga tggccgacgt cgataaaccc ggctcggact cgatcgtggg
     1801 caacatgatt gcgacctgga tggaaaaggt cggaagcccg caggaaccgg tcacggttga
     1861 aagacgcgtc caggaagacg gtaccgcatc gcttcttgtc gtgaccaggc gaaacggcgg
     1921 cgctgtcaga acctggcttg atcacaagac tttcgaacag cacgaagtcc tgcagttccg
     1981 caaggtgatg aacgacctgc gcaaggttgc ccactggccc taccatgtga ccctgggcga
     2041 gcgtgacatg caggtcacaa tgccggacga actgctgacg ctgatcatgg atcgcggcca
     2101 gaagggcatg agctaccaga ggtacaaggg tctcggcgaa atgaacgccg ggcagctctg
     2161 ggaaaccacg atggatccgg cgcgccggac gcttctgaag gtcaatatcg tcgatgcgat
     2221 caaggccgac gaaatcttca cgctcctgat gggcgacgcg gtcgagccgc gccgcgaatt
     2281 cattaacgaa aacgccctca acgtcatcaa ccttgacgtc tagggccagc cactggaagt
     2341 cttgcagatg acatcttacc tggaagacgt tgaatctctc tttctgtccg ccgtcagaaa
     2401 gggtctgtcg cttcgcgtct ccgacctgga aatcgtgcgc gactgggatc ggcgccgggt
     2461 tcccatcgac gttgtttcaa gggggattct ttacggaatt caacgatttc tggcacaggc
     2521 cgagccggac gcaccggttc cggcgagcct gaagtactac cgcacggcgg tcgagaagga
     2581 attccagaca tgggaacgcg cggcggccag gggacttgcg atgacctccg cggtgccgca
     2641 agccgccagg aaggccggcc ctgacctgcc tttgaccgag agggcacgga acatcctgct
     2701 gcagagaatc agcgacgcga cgggagccac gcgtgacgta tatgccgaag cattgaacca
     2761 ctatgatgcc gcggtgaagt caacgccgct tgcggacctg ctgttccagg ttgaagatat
     2821 ggtggtggcc ggcctgggga aaaacctggg ggatgaacgc cttgccgcaa tcgccgccgc
     2881 ctcggccggg caggcggcgg ccgccgccgc aaggggcgtg gggccaaggg cgctccggga
     2941 tctggagagg aatctttttc gcaaggcggt tgcggccgag ttgggttttt caagcttcct
     3001 tgacgaggtc ctggacaggc cgggcaggaa gtgaccggtc gggggcgttg caatgaaatg
     3061 tgccagatgc aaggacctgg gatacgtcac ggcgccgctt ggtgaattcg ccgtcgcgcg
     3121 cgtgtgcgaa tgtcggaccc cgtgccccga atgcaacgga accggcctga taatcgacgc
     3181 atcaaccctt ccccgcaagg cggcagtctg ttcgtgccgt cacctgaaca ggcgggtgga
     3241 actcttcaac agggcgggac tgccggccag gatgcattca aagacgcttg atctgttcga
     3301 ggagcgcagc cccgcgcagg cgcgcacgaa gacgttcatc cgccgcttca ccgaggacta
     3361 cgccccgggg aaccgcggat tcctgctttg gggaaagccc ggcaccggca agactcacct
     3421 ggtctgctcg atgtcgcggc acttcctgct tgagatgggg tggtcggtca aattcgtcga
     3481 cttcttccag cttctttccg acctgaaggc cggtttctcg gacggtcgct cggaggccga
     3541 gctgatatca ccgcttgtcg ccccggacgt gctgataat
//
LOCUS       DAOPPA010000342         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28464_length_3562_cov_4.420017, whole genome shotgun sequence.
ACCESSION   DAOPPA010000342 DAOPPA010000000
VERSION     DAOPPA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28464_length_3562_cov_4.420017"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /locus_tag="PKH54_10220"
     CDS             <1..2998
                     /locus_tag="PKH54_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00314.1"
                     /translation="ADVFQRLLDMSNEVPDRILYCNRLGVLYHDALGDDRKALDYYLQ
                     VLDLDPEDDEVFETAVGLLTKMEDWSELVNLYESRISRVEDVDVRIATLTREADVYEK
                     KQEDINSAITCFQRMLNYRPDYVPGFENLIRLFEQMEAWEDVVDTYLKWKEQVDSDHH
                     MVDLMMKAVDIVRHKLENPDRAIEMLKGIMVVDPTNQVASKCLIDIYGELEDWEKVAG
                     VHLGMEPYLKTDDEKARARAQAGDIYLNKLKDRSHAIEHFERALEINPKLSDVALALA
                     STYVASARWEKAEPLLDLLVSDSAVMADAVRAADIHYNMGLCAEKLFDYERAFREYTM
                     AVKTRPGHFRTVLGLGRYYQRKELWQLAKDNFMKAVINGREELTDDEKIEIGFALGEI
                     NLALNDEREAGIQLDKVLALAPNNEKAVDLQIAIAERRKDWPAVIRYKQARVDARGDA
                     YERFSVMLEIGDIYKEKMQNVHGAMAAYKEALSIDPGAKVALMRLYNLHLESGAFEDA
                     LYMLNSMALAEENMEQRSRVYVQMAALYQEKLGDDVSAIEYLNLALDADPDRLEAFRA
                     IDEVLTQKKDWDGQAKAYSRMLERLAGRGNIELEYRLHANLGEIYRSRLKNMDMAIFE
                     YQRASQIKPSEKRTYEILAQLFEVTEDATDKAIDAHRQIVMLGPISKDAAGSYKAMQR
                     LYMQRGDFDRAFVVSSIMTAMGIADEKEKKLYEDNLEPGVPWFKGTIDPLRWETHLMA
                     KDENVLLGRTLQVLFQGVGSYLDVKELKDLGLRRKPDMDMDQKLMFVNIYKAVNKALG
                     PLPHKVYRDDNPTGMKVDFLVPPALVVGADMMTGHDEYELAFQIGRQLTFLHPMHFLA
                     SVKNLTELKILMAAVFKYCKPEMELGTGADVVMELSRTIDRKMGQQQKNQMTKLVDDL
                     MKKYPMSLDDMFRDFFRSIEVTQLHAGTLVACNVPSVMNFLRTEQSGFSNMTQRERIE
                     EVLRFAISDDHYILRSALGINLESRA"
     gene            complement(3285..>3562)
                     /locus_tag="PKH54_10225"
     CDS             complement(3285..>3562)
                     /locus_tag="PKH54_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD00315.1"
                     /translation="ADFFDSAPPCPSGLLVLNPPYGLRLDATRDAAALYRRIATTISS
                     RWAGWRYAVVMPDQSLARTWPLQTDSSLRFMHGGLKAVALIGTTGRM"
BASE COUNT          794 a          959 c         1126 g          683 t
ORIGIN      
        1 ggccgacgtt ttccagaggc tcctggacat gtcgaacgag gttccggaca ggatccttta
       61 ctgcaaccgc cttggcgtcc tttatcacga cgcgctgggc gacgaccgga aggccctgga
      121 ttactacctg caggtactgg atcttgaccc cgaggacgac gaagtcttcg agaccgcggt
      181 cggactgctg acaaagatgg aagactggtc cgaactggtt aacctgtacg aaagcaggat
      241 cagccgcgtc gaagacgtcg atgtgcggat cgccacgctg acccgcgagg ccgatgtcta
      301 tgaaaagaag caggaggaca tcaattccgc gatcacctgt ttccagcgga tgttgaatta
      361 tcggccggat tacgtgccgg gatttgagaa cctgatccgc ctgttcgagc agatggaggc
      421 atgggaagac gttgttgata cgtacctgaa gtggaaggaa caggtcgatt cggatcacca
      481 catggtcgat ctgatgatga aggccgtcga tattgtccgc cacaagctgg aaaacccgga
      541 tcgcgcgatc gagatgctga agggcatcat ggttgtcgat ccgaccaatc aggtcgcttc
      601 gaagtgcctg atcgacattt atggcgaact ggaagactgg gagaaggttg ccggtgtcca
      661 tcttggcatg gaaccctacc tgaagacgga tgacgaaaag gcgcgcgcaa gggctcaggc
      721 cggcgacatc tacctgaaca agctgaagga tcgttcacac gccatcgagc atttcgaaag
      781 ggcgctggaa atcaatccga agctgtcgga cgtggcgctt gcgctggcct cgacttatgt
      841 cgcgtcggca cgctgggaaa aggcggagcc gctgctggat ctgctggttt ccgattccgc
      901 ggtgatggcc gatgcggtcc gtgccgccga catccattac aacatgggtc tgtgcgcgga
      961 aaaactgttc gattacgagc gtgccttccg tgagtacacg atggccgtca agacccggcc
     1021 cggtcatttc cggacggttc tgggacttgg tcgttactac cagcgcaagg aactgtggca
     1081 gcttgccaag gacaacttca tgaaggccgt catcaacggc cgtgaagaac tgaccgatga
     1141 cgagaagatc gaaatcggct tcgccctggg cgaaatcaac cttgccctga acgacgagcg
     1201 cgaggccgga atccagttgg acaaggtcct ggcgcttgcc ccgaacaacg agaaggccgt
     1261 cgatctgcag atcgcgattg ccgagcggcg caaggactgg ccggcggtca tccgttacaa
     1321 gcaggcaagg gtggatgcgc gcggcgacgc ttacgagcgt ttcagcgtga tgctggaaat
     1381 cggcgacatc tacaaggaaa agatgcagaa cgttcatggc gcgatggcgg cgtacaagga
     1441 agcgctgtcg attgatccgg gcgccaaggt ggccctgatg cgcctgtaca acctgcacct
     1501 ggaaagcggc gctttcgagg acgcgcttta catgctcaac agcatggcgc tggccgaaga
     1561 aaacatggag cagcgttcgc gcgtctatgt ccagatggcc gccctttacc aggaaaaact
     1621 gggtgatgac gtcagcgcca tcgagtacct gaaccttgcc ctggatgcgg atcccgaccg
     1681 tcttgaagcg ttccgggcca tcgacgaggt cctgacccag aagaaggact gggacggaca
     1741 ggccaaggct tacagcagga tgctggaaag gctggccggt cgtggcaaca tcgagctgga
     1801 atacaggctt cacgcaaacc tgggtgaaat ctaccggtcg cgccttaaga acatggacat
     1861 ggcgatcttc gaataccagc gcgcatcgca gatcaaaccg tccgaaaagc ggacgtacga
     1921 gatcctggcg cagttgttcg aggttaccga ggacgccacc gacaaggcga tcgatgccca
     1981 caggcagatc gtgatgcttg gcccgatttc gaaggatgcc gcgggttcgt acaaggcgat
     2041 gcagcgtctt tacatgcagc gtggtgattt tgatcgggcg ttcgtggttt cgtcgatcat
     2101 gacggcgatg ggaatcgcgg atgaaaagga aaagaagctg tacgaagaca acctggaacc
     2161 cggcgtgccg tggttcaagg gaaccatcga tccgctgcgc tgggaaaccc acctgatggc
     2221 caaggacgaa aacgtcctgc ttggccggac tctgcaggtg cttttccagg gcgtcgggtc
     2281 gtacctggac gtaaaggagc tgaaggacct tggcctgcgc cgcaagccgg acatggacat
     2341 ggaccagaag ctgatgttcg tgaacatcta caaggccgtc aacaaggcac tgggcccgct
     2401 gccgcacaag gtttatcgcg atgacaaccc gaccggcatg aaggttgact tcctggtgcc
     2461 gccggcactg gttgtcggcg cggacatgat gaccggccac gatgaatacg aactggcgtt
     2521 ccagatcggt cgtcagttga cgttcctgca cccgatgcat ttcctggcct cggtgaagaa
     2581 cctgaccgaa ctgaagatcc tgatggctgc cgtgttcaag tactgcaagc cggaaatgga
     2641 actgggtacc ggcgcggacg ttgtgatgga attgtcgcgc acgattgacc gcaagatggg
     2701 tcagcagcag aagaaccaga tgacgaagct ggtcgatgac ctgatgaaga agtacccgat
     2761 gtcgctggac gacatgttca gggacttctt ccgcagcatc gaagtcaccc agcttcacgc
     2821 cggtactctt gttgcctgca acgtcccgtc ggtcatgaac ttcctgcgca ccgagcagag
     2881 cgggttctcg aacatgaccc agcgcgagcg catcgaggaa gtcctgcggt tcgccatcag
     2941 cgacgatcac tacatcctgc gcagcgccct cggcatcaac ctggaaagcc gcgcctgatc
     3001 aattgataag caatacgtct ggattttgag ggcggatctt cattcgcccc tatcgggcct
     3061 cgtggcttga attgacgggc gaatttgccg ccctgagtca gtggaagagg ctttcatccc
     3121 tatagccagg atcgtgtctg gaaaggcgaa atttcattcg catccacagt cgagtggtgc
     3181 gcgtcaacgg cttgtccctg tggcttgtat gggcggatta catccgcccg ctgcggtcga
     3241 gcagtgcgtg tcgccggcat gtccctgtgg cttgtatggg cggattacat ccgccccgtc
     3301 gtaccaatca aagcaacggc tttcaggccg ccgtgcatga atcgcaggga tgagtccgtt
     3361 tgaaggggcc aggtgcgggc gagtgactgg tcgggcatga cgacggcgta gcgccagcct
     3421 gcccagcggg aactgatggt ggtcgcgatt cggcggtaca gtgcggcggc atcgcgggtg
     3481 gcgtcaaggc gcaggccgta aggcgggttc agaaccagaa gtccggacgg gcagggcggg
     3541 gcggagtcaa agaagtcggc cg
//
LOCUS       DAOPPA010000343         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28466_length_3562_cov_3.844882, whole genome shotgun sequence.
ACCESSION   DAOPPA010000343 DAOPPA010000000
VERSION     DAOPPA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28466_length_3562_cov_3.844882"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKH54_10230"
     CDS             <1..1066
                     /locus_tag="PKH54_10230"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00316.1"
                     /translation="TGGFLVDVKVKIGTVMYSGNTVAKDENRLYVSATAGVKGPITVY
                     DKDNKVATIAGPFRQTEKGVVACDGSTPAASGKVPDNPWAAGPYAAGYVDISGDSSKT
                     RVYYPAECGSVRRPAAEGTWPLVVILHGNGALHLQYEYLAELLATWGFVTIVPETIQN
                     LAGEDYSQLIFNTGPLVQSFRGVNLGSKNAVLQKVTTTSEVFWIGHSRGTGRIEELCH
                     VNDYGEWDATVGELFLGPVDDGIQAPGLTMIIGATKDMQSTPATYKNTYNTQAGIKWL
                     VDINGGNHGSFCDHKVYGYGVMGGMGDKEPTISRHRQHVIVQMFAVPLIQRAFGLDEP
                     FADYLETPPVSGDYSFEYDD"
     gene            complement(1077..3116)
                     /locus_tag="PKH54_10235"
     CDS             complement(1077..3116)
                     /locus_tag="PKH54_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00317.1"
                     /translation="MKSFNPSIHCLVILLVVMGATACGDGSGPTMDNGATDTVEMDGN
                     DTPDGQQPDLVVNPDAARDLAGHDLAGIDLVGADLNNPDLPFNQDNGSTDNGDGDTAI
                     LPPCTDSAQCQLGNVCIDRQCVPGCNGDRDCPGNQHCKSDALPYGACLDCVTDDHCDD
                     DMKCNAGKCMVTCEVNEECSATPITPFCDYDSGLCVKCLSDSNCPEGRLCIGRDCIVG
                     CKGDRDCPDGLRCDPKFGTYGDCFTCVEDADCNGKVCRDHQCVIDCDAINCPVERPIC
                     DPATGNCLECIKKADCGTGKVCIGNLCITGCESDLDCGTRHCSNGSCVECTIDDHCTG
                     GQKCRANTCSAGECFKDSDCNPGDYCHPLLYSCEDLPSNHCDSNSDCTSWIPGLLDQH
                     CDPLTRECITACLSGFCLDLLGSGRDTCVNGGCYGCGTDFDCAGVRCSPYDRFCYACQ
                     DNDDCSVPGWKCAQDGACYECLSSFDCPSPKVCDTANGRRCVECLTSGDCKNPSKPVC
                     GKSKTCIPACQNECESGDMICNPDDTTFPITALTCGDTDDDPCLEYGGYYNCGNGASC
                     VTQGSGLGECVCNNECSSGQKWCASGKTDVVETCKQDSTSGCWYISTSYCSSSYGEIC
                     SNGTCVCDNKCTENQTVCTSTTAYKKCVSDYYTGCLYWTSYTCSSGYYCSNGKCQ"
     gene            complement(3254..>3562)
                     /locus_tag="PKH54_10240"
     CDS             complement(3254..>3562)
                     /locus_tag="PKH54_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD00318.1"
                     /translation="YRNRLTRRKTTRLPPNTYWPGDSNYFEVGSDTVAPLVARFLQHQ
                     RHQAGLTLVEVARRLGSASPNAYARYEQGRCVPSMSKLSKLISVLNDGKGVVIEMPRS
                     "
BASE COUNT          681 a         1004 c         1109 g          768 t
ORIGIN      
        1 taccggcggc ttcctggtcg atgtgaaggt caagatcggc accgtgatgt attccggcaa
       61 caccgtggcc aaggatgaaa acaggcttta tgtaagcgcg acggccggtg ttaaaggccc
      121 gattaccgtt tatgacaagg acaacaaggt ggcgacgatc gccggtccat tccggcagac
      181 cgaaaagggc gttgtcgcgt gtgacggatc gacgccggct gcaagtggaa aggttcccga
      241 caatccgtgg gctgctggtc cgtacgcggc gggatatgtc gatatttccg gcgattcctc
      301 caaaaccagg gtttattacc ccgcggaatg cggttccgta agacgtccgg ctgccgaagg
      361 tacgtggccg ctggtagtca ttctgcatgg aaacggggcg ctgcacctgc agtacgaata
      421 cctggccgag cttcttgcaa cctggggatt cgtcacgatt gtcccggaaa ccatccagaa
      481 cctcgccggc gaggattatt cccagcttat cttcaacacc ggtccgctgg ttcagtcctt
      541 caggggcgtc aatcttggca gcaagaatgc ggtgcttcaa aaggtgacga caacgtcgga
      601 agtcttctgg attggtcatt cgcgcggaac cgggcgcatc gaggaattgt gtcacgtcaa
      661 tgattacggc gagtgggatg caacggtcgg cgagcttttc ctggggccgg tcgatgatgg
      721 aatccaggcg ccggggctga caatgatcat cggggcgaca aaggatatgc agtccacgcc
      781 cgcgacttac aagaacacct ataacaccca ggcgggcatc aaatggctgg tcgatatcaa
      841 cggcggcaac cacggatcct tctgtgacca caaggtttac ggatatggcg tgatgggcgg
      901 catgggcgac aaggagccga ccatcagccg tcaccgtcag cacgtcatcg tccagatgtt
      961 cgcggttcct ctgatccagc gtgccttcgg cctggatgaa cctttcgccg actacctgga
     1021 aaccccgccc gtcagtggtg actacagctt cgagtacgac gactgagccg tcgcggttac
     1081 tggcatttgc cgttgctgca gtaatagccg ctgctgcaag tgtagctggt ccagtacagg
     1141 cagcctgtgt agtagtcgga cacgcacttt ttgtaggcgg tcgtgctggt gcagaccgtc
     1201 tggttttcgg tgcatttgtt atcgcagacg catgttccgt tgctgcagat ttcgccgtag
     1261 cttgacgaac agtagcttgt gcttatgtac cagcagccgc tggtggaatc ctgcttgcat
     1321 gtttcaacga cgtccgtctt gccggatgcg caccacttct ggcctgaact gcattcattg
     1381 ttgcagacgc attcgccgag ccctgagccc tgggtgacgc acgacgcccc gtttccgcag
     1441 ttgtagtaac cgccgtattc caggcagggg tcgtcgtcgg tgtcgccgca ggtcaacgcc
     1501 gttatcggga aggtggtatc gtccgggttg cagatcatgt cgccggattc gcattcgttc
     1561 tggcaggccg ggatgcatgt ctttgatttg ccgcacaccg gtttggatgg attcttgcag
     1621 tcgccgctgg tcaggcactc gacgcagcgc cggccgttgg cggtatcgca gaccttgggc
     1681 gaggggcagt cgaagctgct caggcattca tagcaggcgc cgtcctgggc gcacttccag
     1741 ccgggcaccg aacagtcgtc gttgtcctgg cacgcgtaac agaagcggtc gtacgggctg
     1801 cacctgacgc cggcacagtc gaagtcggtg ccgcagccat agcagccgcc attgacgcac
     1861 gtatcgcgcc cgctccccag cagatccagg cagaatccgg acaggcaggc cgtgatgcat
     1921 tcgcgggtca gcgggtcgca gtgctgatcc agcagtccgg ggatccatga agtgcagtcg
     1981 gagttgctgt cacagtggtt gctgggaaga tcctcgcaag aatacagcag cgggtggcag
     2041 tagtcccccg ggttgcagtc gctgtccttg aagcattcac cggccgaaca tgtgttggcg
     2101 cggcacttct gcccgccggt gcagtggtcg tcgatggtgc attccacgca ggatccgtta
     2161 ctgcagtggc gagttccgca atccagatcg gattcgcatc cggtgatgca aaggtttccg
     2221 atgcagacct tgccggttcc gcagtcagct tttttaatgc attccaggca gttgcctgtt
     2281 gctggatcac agattgggcg ttcgaccggg cagttgattg catcgcaatc gatcacgcac
     2341 tggtggtcgc ggcagacctt gccgttgcaa tcggcgtcct cgacgcaggt gaagcagtcc
     2401 ccgtatgtcc cgaatttcgg atcgcagcga aggccgtccg ggcagtcccg gtcgcccttg
     2461 catcccacga tgcagtcgcg gccgatgcac aggcgccctt cggggcagtt gctgtcggac
     2521 aggcacttga cgcaaagacc ggagtcatag tcgcagaatg gggtgattgg cgtggcgctg
     2581 cactcctcgt tgacttcaca cgtgaccatg cattttccgg cattgcactt catgtcgtca
     2641 tcgcagtggt cgtcagtaac gcaatcaagg caggcgccgt agggaagcgc atctgacttg
     2701 cagtgctggt tgccggggca gtcacggtcc ccgttgcaac cggggacgca ctggcggtcg
     2761 atgcagacgt tgcccaactg gcattgagcc gagtcggtgc aaggcggcag gatggcggtg
     2821 tcgccgtcgc cgttgtctgt tgagccgtta tcctgattga agggcaggtc gggattgttc
     2881 agatccgcgc cgaccagatc tattccggcc aggtcatgtc cggccaggtc gcgggccgcg
     2941 tcgggattga cgaccaggtc cggctgttgt ccgtcgggag tgtcgtttcc atccatttcg
     3001 acagtgtcag tggctccgtt gtccatggtc gggccgctgc cgtcaccaca ggcggtagca
     3061 cccatgacaa caagaagaat cacaagacaa tgaattgagg gattgaagct cttcatctcg
     3121 attccttctg aaaagtccgg aaagtccgga aattgtatcc catcggaagc agcgttgaaa
     3181 gggtgccaca gcggcaggcg ccccactgat gtggatgcat gccgaccgtc tgtgtccaac
     3241 gatcgccggc atcctacgat cgcggcattt cgatgacgac gcccttgccg tcgttcagga
     3301 cgcttatcag tttcgacaac ttggacatgg aggggacgca ccgtccctgt tcatagcggg
     3361 cataggcatt tggcgaagca gaaccgagcc gccgcgccac ctcaaccagg gttagtcccg
     3421 cctggtggcg ttgatgctga agaaaccttg cgacaagggg cgcgacagtg tccgagccca
     3481 cttcgaagta gttcgagtcc ccgggccaat aagtgtttgg gggcaagcgc gtcgttttgc
     3541 ggcgcgtcag tctgtttcga ta
//
LOCUS       DAOPPA010000344         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28550_length_3554_cov_3.136896, whole genome shotgun sequence.
ACCESSION   DAOPPA010000344 DAOPPA010000000
VERSION     DAOPPA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28550_length_3554_cov_3.136896"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1908)
                     /gene="recN"
                     /locus_tag="PKH54_10245"
     CDS             complement(223..1908)
                     /gene="recN"
                     /locus_tag="PKH54_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOD00319.1"
                     /translation="MLKTLHVRNLGVLGDVEVDFHPGLTVLTGETGAGKSLLVGAIKL
                     LMGARAEGSDVRSGESQAVVEAVFSTGSSPEIDSLFAEAGYGPYDGEEGVIHVRRTIT
                     SDGRSKAYINGSMASARVLRDLVGALVSVAGQHAFIGLGVPGQRMAMLDAFACNQSLT
                     ADCRDAFKAFSEAAARLRRLDEAAAGREARRDYLRHVISQIESVGLKPGDGDSIRDEA
                     ARLRGSGRLRELAAQGYEALYEGAGAVFDELGRVEHLLREMAAIDRGLEPMLARLDSA
                     RIEVRELSREILDYGEGVDLDPERLVAVEARLDAMQDLFRKYGGSEDAVLRTLAAARA
                     ELDGLDGDVVEGEGLAGKVAGLKSMFMKTAGVLGEERRRHAAILGAAVTAVIRQLAME
                     GATFRVDVNPDEPGPDGCDRVEFMIEANPGEGFGQVADVASGGELSRITLGIYSVMSG
                     SVGTPVIVYDEIDAGVSGGVAERMGQVLKKASEGRQVLVVTHHGQVAACGDEHFKVFK
                     SLVGGRTVAGVEGLTGQARVDEIARIIGGVVITDKAREHAGEMLGSSPDGLLL"
     gene            complement(1994..3067)
                     /gene="prfA"
                     /locus_tag="PKH54_10250"
     CDS             complement(1994..3067)
                     /gene="prfA"
                     /locus_tag="PKH54_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD00320.1"
                     /translation="MDLSVALRKLDDVEARYERLNADMEMPEVYLNVDRMKEIGLERA
                     ELEETVSMYREYRHVMQAISDNEELRSDPDLGELAVEELKRLIPRGEELESALMRFLV
                     PVDPLDKKNIFLEIRAGTGGEEAALFAANLFRMYCRYAEARRWAVEIVNISETELGGL
                     KEVIALVSGSRVYSYLKFEGGVHRVQRVPETEAQGRIHTSAVTVAVMPEADDDFEMEI
                     DKSEIREDIFRASGAGGQHINKTDSAIRLTHLPTGMVVTCQDERSQHKNRAKAYKVLA
                     ARLAEKKRSEQYASEQDARRAMVGSGDRSERLRTYNFPQNRLTDHRINLTLYKLEQIM
                     EGDLDEMISNLQAQFAAMALAER"
     gene            complement(3070..>3554)
                     /locus_tag="PKH54_10255"
     CDS             complement(3070..>3554)
                     /locus_tag="PKH54_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOD00321.1"
                     /translation="ALSPAELESRTTLHPRCGTAFLVTVIAVSIFVFAGVFPLLPELS
                     QVRFVNQVLYVAIKLPLILPVAAISYEIIRFAGKCRGSWFGSMVSGPGLLMQKITTRR
                     PDARQQEIAIVALESALNPALFGLSGEFGERVAEFAGYDEFREFSNRRAAGPEESSGE
                     "
BASE COUNT          747 a         1192 c          941 g          674 t
ORIGIN      
        1 gtatcaatac ttgaatccgt caggacgtcg gtgggacttg aaccggaacc gcaggcgacg
       61 acaaacagcg gaacaagagc cgcaaggaat gacttattca tcggaccccc gacagaaaat
      121 cccacaagac aaccgaaccg gtttccaagg acacgggaac accggccggg acagaagcca
      181 ttataaggat atggttcccg tctgcaacgg caacgattgc gcctaaagca gcagtccgtc
      241 cggggacgat ccaagcattt cgccagcgtg ttcccgggcc ttgtcggtga tcaccacacc
      301 accgattatc ctggcgatct cgtcaaccct ggcctggccg gtcaatccct cgacgccggc
      361 cacggtgcgt ccaccgacaa gactcttgaa taccttgaaa tgctcgtcgc cgcaggcggc
      421 gacctggcca tggtgcgtca cgaccagaac ctggcgaccc tcggatgcct tcttcagcac
      481 ctgccccatc cgctcggcaa cacccccgga aacgcccgcg tcgatttcgt catacacgat
      541 caccggcgta ccgaccgatc ccgacatgac cgaataaata ccgagcgtta tacgagaaag
      601 ctcaccgccg gaagcgacat cggcgacctg gccgaaccct tcacccgggt tcgcctcgat
      661 catgaactca accctgtcgc atccatcggg ccccggttcg tccggattga cgtcgacgcg
      721 gaaggtggcg ccctccatag ccagttgacg aataacggcg gttaccgcgg ccccgagaat
      781 tgccgcatgc ctgcgccttt cctcgccaag aaccccggcg gtcttcatga acatcgactt
      841 cagcccggca accttcccgg ccagaccctc accctcgacg acatccccat ccagaccgtc
      901 aagctcggcc ctggcggcgg ccagcgtcct caagacggca tcctcgcttc ctccgtactt
      961 gcggaaaaga tcctgcatcg catccagtcg cgcctcgacg gcaaccaggc gttccggatc
     1021 aagatcaaca ccctcgccgt aatccaggat ttccctggac agttcacgaa cctcgatcct
     1081 ggcggaatca agcctggcca gcatcggctc aagccccctg tctatcgcgg ccatctcgcg
     1141 caacaggtgt tcaaccctgc ccagctcgtc aaaaacggcg ccggcccctt cgtaaagagc
     1201 ctcatatccc tgggcggcaa gttccctcaa cctgccggat ccgcgcagcc gcgccgcttc
     1261 gtcacgaatc gaatcgccgt cgcccggctt caacccgacc gactctatct gggaaatgac
     1321 atgtcgcaga taatcgcgcc tggcctcgcg accggcagcg gcttcatcaa gcctgcgaag
     1381 tcttgcggcg gcctcgctga atgctttgaa tgcatcacga caatcagccg tcagactctg
     1441 gttgcaggcg aaggcatcga gcatcgccat gcgctggccg ggaaccccga ggccgatgaa
     1501 ggcgtgctga ccggcaaccg acacaagcgc cccgaccaga tcccgaagaa cccgggccga
     1561 agccattgaa ccgttgatgt atgccttgga tcggccgtcg gaggttatcg tcctgcgtac
     1621 gtggattacc ccttcctcgc catcgtaagg gccataacca gcctcggcga acagggaatc
     1681 gatctccgga ctggaaccgg tcgaaaagac cgcttccaca acggcctgac tttcacctga
     1741 acggacgtca gaaccttcgg cccttgcacc catcagcaac ttgatcgccc cgaccagcaa
     1801 cgacttgccg gcaccggtct ccccggtcag gacggtcagg cccggatgga aatcgacctc
     1861 gacatcgcca aggacgccaa gattcctgac atgaagggtc ttgagcaaca gtccctccgc
     1921 cggcaaccat gcggccggtc gaacccggat tcagacatgg ggattgtatt ccatcgcggc
     1981 ggcgtgaaac aggttatctt tcggcaagcg ccatcgcggc gaactgggcc tgaaggttgc
     2041 tgatcatttc gtccaggtcg ccttccatta tctgttccag cttgtaaagc gtcagattaa
     2101 ttcgatgatc ggtcaacctg ttctgtggaa aattgtaggt tctgagtcgc tccgaacgat
     2161 ccccggatcc gaccatggcc cgccgggcat cctgctccga ggcgtactgc tccgagcgct
     2221 tcttttccgc aagcctggct gcaagcacct tgtacgcctt cgcccggttc ttgtgctggg
     2281 atctttcgtc ctggcaggta acgaccatgc ccgtcggaag gtgcgtcaac cttatcgccg
     2341 aatcggtctt gttgatgtgc tggccgcccg cgcccgaggc acggaagatg tcttcgcgga
     2401 tttcgctctt gtcgatctcc atttcgaagt catcgtccgc ttccggcatg acggcgaccg
     2461 ttacggcgga cgtgtggatg cgcccctgcg cctcggtctc gggcacccgc tggacgcgat
     2521 gcacgccgcc ctcgaacttg agatagctat agacgcggct tccgctgaca agcgctatga
     2581 cttccttcag gccgcccaac tcggtctcag agatgttgac aatctcaacg gcccagcgac
     2641 gggcctccgc atagcggcaa tacatcctga aaaggtttgc cgcgaacagc gcggcttcct
     2701 cgccacccgt tccggccctg atttcaagga aaatgttctt tttgtccagc ggatcgacag
     2761 gcaccaggaa acgcatcaac gcggattcca gttcctcgcc gcgggggatc aaccgcttca
     2821 attcctcgac cgccagttca ccgagatcag gatccgaacg cagttcctcg ttgtcggaaa
     2881 tggcctgcat gacgtgccgg tactcacgat acatcgacac ggtttcctcg agttcggcgc
     2941 gctccaggcc gatctccttc atgcgatcga cgttcagata gacttcaggc atttccatgt
     3001 cggcattcag gcgctcgtac ctggcctcca cgtcatccag cttccgaagc gcaaccgaaa
     3061 gatccatcgt cattctcccg aactttcttc cggcccggcg gcccggcgat tcgaaaattc
     3121 ccggaattca tcgtaaccgg caaattccgc gaccctttcc ccgaattccc cggaaagccc
     3181 gaacaaggca gggttcaacg cggactcaag ggccacgatc gcgatttcct gctgacgcgc
     3241 gtcgggacgc ctggtcgtga tcttctgcat gagcagcccg ggtccggaaa ccatgcttcc
     3301 gaaccatgac ccccggcact tcccggcgaa ccttatgatt tcataggaaa tggccgcaac
     3361 cggcaggatc aggggcaatt tgatggcgac atacagaacc tggttcacga acctgacctg
     3421 cgaaagctcg ggaagcaacg ggaacacgcc ggcaaaaacg aatatgctga ccgcgatcac
     3481 cgtgaccagg aaggccgtgc cgcaccttgg atgaagggtt gtgcggctct caagctctgc
     3541 cggcgaaaga gctg
//
LOCUS       DAOPPA010000345         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28587_length_3551_cov_3.757723, whole genome shotgun sequence.
ACCESSION   DAOPPA010000345 DAOPPA010000000
VERSION     DAOPPA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28587_length_3551_cov_3.757723"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKH54_10260"
     CDS             <1..518
                     /locus_tag="PKH54_10260"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOD00322.1"
                     /translation="RTLLLSNAAGGVNPDFRTGDLMLITDHINMMGDSPLRGPNPGNL
                     GPRFPDMTDTWTPSIRESFTRQAEALGIDLRQGIYLANSGPAYETPAEVRMARVLGAD
                     AVGMSTVPEAVAARHAGMDVGGVSLITNMAAGMTNGRLSHEEVKAVGAASAARLGDLF
                     KAVIESLQAPR"
     gene            529..939
                     /gene="cdd"
                     /locus_tag="PKH54_10265"
     CDS             529..939
                     /gene="cdd"
                     /locus_tag="PKH54_10265"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOD00323.1"
                     /translation="MLQRDEDAMLEAARQARENAWAPYSGFRVGAAVLGASGRIYPGC
                     NVENASYPVTTCAERNAIGAAISAGERRIVAVMVCADHKDPVTPCGMCRQAIAEFGPD
                     VRVVCVGRGDDRVEYSVSELLPFAFGADKLEGRA"
     gene            936..2282
                     /locus_tag="PKH54_10270"
     CDS             936..2282
                     /locus_tag="PKH54_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD00324.1"
                     /translation="MMAHNDRARQILKGLTIVTVNGTNQVLSGDIAIMDGMIQAVGPS
                     LPADPEDTVLDMEGMLAMPPFVQTHVHLCQTLWRNMADDMSLIQWLKERTLPLEAAMT
                     ASTVRSSVLLSAAELLLSGTLLVNDFGLAGLVPDIARAVSGTGLRAVTGRILMDLHDG
                     PPQLRQDASASIADAIATEREITGLPDIVGCSINPRFAVSSSMRLLEMVREAAGRTRM
                     AIHTHASENEQENELTVARFGARPIRLYEKLGMLGPALRLAHCVKVDNDEIELIARTG
                     TKVMHCPSTNLKLGSGIAPIPRMLEAGIPVSIGADGAPANNNLDMFNEMRIASLIQKP
                     EHGPDCMPAWQILRMATINGAAALGLQHETGSIEPGKRADILILNPHLAHSTPFDDPV
                     PAIVYSMGRENVRHVLSKGVFVVRDGKCELFGIDSAIAMAQMGSRDLRRRSSIDFK"
     gene            complement(2265..2801)
                     /locus_tag="PKH54_10275"
     CDS             complement(2265..2801)
                     /locus_tag="PKH54_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOD00325.1"
                     /translation="MNGERHRLIVGRLAVLAAACVLAALLRLAGSGFDSSSRITGDTV
                     IETVRRDGASSFILLEGPATVCGVLDQAGIRTRCRSDRQLKSGDAIFEVGLDSWRVGI
                     MDGRSLILFGLPIPVNRAGADDLVAIDGIGPARAAAIVTDRNVRGPFLCPGDLSRIKG
                     IGQATAARISAWMSFEVD"
     gene            complement(2898..3551)
                     /locus_tag="PKH54_10280"
                     /pseudo
     CDS             complement(2898..3551)
                     /locus_tag="PKH54_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          683 a         1065 c         1102 g          701 t
ORIGIN      
        1 cgcggactct gctgctttcg aatgcggccg gtggcgtgaa cccggatttc agaaccgggg
       61 atctgatgct gattacggat cacatcaaca tgatggggga cagccctctt cgcggtccca
      121 acccggggaa cctgggcccg agattcccgg acatgaccga tacgtggaca ccatcgattc
      181 gcgaatcttt cacgcgccag gccgaggcgc ttgggatcga cctcaggcag ggaatttatc
      241 ttgccaacag cgggccggca tacgagacgc cggccgaggt acggatggcc cgcgtcctcg
      301 gggccgacgc cgtcggcatg tccaccgtgc ccgaggccgt tgccgccagg catgccggga
      361 tggatgtcgg gggtgtctcg ctcatcacga acatggctgc gggaatgacc aatggacgcc
      421 tttcccacga ggaagtgaag gccgtcgggg cggcatcggc cgccaggctt ggcgaccttt
      481 tcaaggccgt aatcgagtcg cttcaggctc cccggtaaaa ggtgaatcat gctgcaacgc
      541 gatgaagacg ccatgcttga agctgcccgt caggccaggg aaaacgcatg ggcgccgtac
      601 tcgggtttcc gggttggtgc cgccgtgctt ggggcatcgg gacgcattta tccgggttgc
      661 aacgtcgaaa acgcgtccta tccggtcacc acgtgtgccg agcgaaacgc aatcggggcc
      721 gcgatctcgg ccggcgaaag gcggatcgtc gccgtgatgg tatgtgccga tcacaaggat
      781 cccgtcactc cttgcggaat gtgcaggcag gcaatcgccg aattcggccc tgatgtgcgc
      841 gtcgtctgcg tcggccgagg cgacgaccgc gtcgaatatt ccgtgagtga gttgcttcct
      901 tttgcctttg gtgccgacaa gctggaaggg agagcatgat ggcgcacaat gaccgcgccc
      961 ggcagatcct gaaggggctg acaatcgtca ccgtcaacgg gacgaaccag gttctgtccg
     1021 gcgacatcgc aatcatggac ggaatgatcc aagccgtcgg cccgtcgctg ccggctgatc
     1081 cggaagacac cgtccttgac atggaaggca tgctggccat gcccccattc gtccagacac
     1141 atgttcacct ttgccagacg ctgtggcgga acatggcgga cgacatgagc ctgattcagt
     1201 ggcttaagga aaggacgctc cctcttgaag cggccatgac agcatcgaca gtccgttcat
     1261 cggttcttct ttccgccgcg gaactcctgc tttccgggac gctgctggtg aacgatttcg
     1321 gacttgccgg cctggtgccc gacattgccc gggcggtttc cggcaccgga ttgcgggcgg
     1381 tgaccggacg gatactgatg gatctgcacg acgggccgcc acagctccgg caggatgctt
     1441 ccgcttcgat tgcggatgca atcgccacgg aacgtgaaat cacgggactg cccgatatcg
     1501 tggggtgctc gatcaacccg cgtttcgccg tttcgagttc gatgcggctg ctggaaatgg
     1561 tgcgggaggc ggccggacgg acgcgaatgg cgatccacac gcacgcttcc gaaaacgaac
     1621 aggagaacga actgacggtc gccagattcg gcgccaggcc gatccgcctt tatgaaaagc
     1681 tcgggatgct tggcccggcg cttcggctgg ctcattgcgt caaggtcgat aacgacgaaa
     1741 tcgagctgat tgcacgcacc ggaacgaagg ttatgcattg cccgtcgacc aacctgaagc
     1801 tgggttcagg gatcgcgccg attccacgga tgcttgaagc cggaattccg gtatcgattg
     1861 gggccgacgg cgcaccggcc aacaacaacc tggacatgtt caatgaaatg aggattgcgt
     1921 ctctcatcca gaagccggaa catgggccgg actgcatgcc ggcctggcag atcctgcgca
     1981 tggcgaccat caacggagcg gctgcgctcg gccttcagca tgaaaccggt tcgattgaac
     2041 cgggaaaacg cgcggacatc ctgattctta atcctcatct tgctcattcg acacccttcg
     2101 atgatccggt gccggcaatc gtgtattcga tgggccgcga aaacgtcagg cacgttctgt
     2161 caaaaggcgt cttcgtcgtc cgtgacggca agtgtgagct gttcgggatc gattcggcga
     2221 tcgccatggc gcagatgggg tcgagggatc tcagacgccg gtcgtcaatc gacttcaaat
     2281 gacatccatg ccgatatcct tgccgccgtg gcctgcccga tgcctttgat tctggacagg
     2341 tcgcccggac acagaaaggg accccggaca ttgcgatcgg ttacgattgc cgcggccctg
     2401 gccggaccga tgccgtcgat ggcgaccagg tcgtcggcac cggccctgtt gaccgggatc
     2461 ggcagtccaa agagaatcag gcttcggcca tccatgatgc cgacccgcca ggaatcaagc
     2521 ccgacctcga atatggcgtc cccggatttc aactgccgat cgcttcgaca ccgcgttctt
     2581 atgccggcct ggtcaagcac cccgcatacg gtcgccggtc cttccagaag aatgaagctg
     2641 ctggcgccat cccggcggac cgtctcgatc acggtgtccc ctgtgattcg gctggacgaa
     2701 tcgaacccgc tgcccgccag cctgagcaat gccgcaagca cgcatgcggc ggcgaggacg
     2761 gcaagcctgc cgacgatgag ccgatgacgt tccccgttca tcggggcacc atcgggctgg
     2821 ttatgttgga ttgattattc ctggacggcc ggagcgtcga gcatctggat ttcgaccttg
     2881 ttgacaagtg ccgggtatcc ggtgcccgcc gggatgagac gacccatgat gatgttttcc
     2941 ttaaggccgc gaagtctgtc ttccttccca cagatggccg cctcggtgag gacacgggtg
     3001 gtttcctgga aggacgccga cgacaggaat gactcgacgt gcagcgaagc cttggtgata
     3061 ccaagcagga tcggctcgcc gaccgccggg gccttgtcct cgacgtaaag acggttgttt
     3121 tcctcttcga atgcccaccg gtcaacatgc tcgtccacca ggaaccggga atcgccggct
     3181 tccgtgatct ttacccaccg aagcatctgt cgaacgatga cctcgatgtg tttatcgtgg
     3241 atgcggacgg actgcaggcg atagacttcc tggacttcgt cgaccaggta gcgcgccagt
     3301 tccttgatgg aaaggacgcg caggatgtcg tgcgggttcg aagctccgtc catcaacgga
     3361 tcgccggccc tgacgacgtc gccttcgcga aggctgacgt actttccctt cgggataagg
     3421 tactcgatct tctcaccgac ttccggggtg atgacgatct tgcgcttgcc cttcgtggct
     3481 ttgccgaacg acacgacgcc gtcgatttcg gagatgatcg cgtgttcctt gggcttgcgc
     3541 gcctcgaaca g
//
LOCUS       DAOPPA010000346         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28597_length_3550_cov_3.674392, whole genome shotgun sequence.
ACCESSION   DAOPPA010000346 DAOPPA010000000
VERSION     DAOPPA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28597_length_3550_cov_3.674392"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKH54_10285"
     CDS             complement(<1..407)
                     /locus_tag="PKH54_10285"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOD00326.1"
                     /translation="MSLEQKLDDAIKDAMRARNQSELACLRMIKSLVAEKRTAPGFTG
                     GVTDELVLGVMSSYSKKLAKTIEEVEKAGHGDNPILQSYRFEIELLKGWLPERLDEVA
                     TRQLVRQIIAEAGLVGANSVGRLTGLVMKSHRDS"
     gene            576..1097
                     /locus_tag="PKH54_10290"
     CDS             576..1097
                     /locus_tag="PKH54_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HOD00327.1"
                     /translation="MKLMSGKVTPLASELVDELVKSENIEVLPEEMPEVQLDLESVLK
                     EYIRTDREITEKARDMISRQKRDYGDLPKVKAQVARERDFGVGENLIEYITAQLIEAL
                     IHSRHVEEVFGMDNDLTLVMTPILKKHLAADEELDQEVRKRIKNLQEGTMTWDAQYQK
                     LKDELKRVRKLDD"
     gene            1175..2101
                     /locus_tag="PKH54_10295"
     CDS             1175..2101
                     /locus_tag="PKH54_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD00328.1"
                     /translation="MRIEGSRIAVTGATGFIGRHIVESLLAAGADVVAVVRNPDKVPE
                     FRRMGIEFRIADLGDRIALTRGFRGVDAVISNAALFRMSNSDWDGHRQANVVGTANVI
                     RAVADAGVTRVVHVSSVAVYKSGGSTPIGEDWPMLEESDLGMFNAYSVSKAVSEREAW
                     KLASKLGLALSCCRPSSVFGPFDPNITPTIARVLSLPVAPIPHLLRAGLVYAGDVADG
                     TLRILRNDSTVGKSYNLTGPHSTSGEFFHAWKDAGGPWPGLAIPVPVPLTRIWNNQRA
                     ERELGWKNRSWQESLCQTFERQPFGRRKKAEG"
     gene            2098..3444
                     /locus_tag="PKH54_10300"
     CDS             2098..3444
                     /locus_tag="PKH54_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOD00329.1"
                     /translation="MMDKTRFRKRRETFFDMMEDGVALIPSGSPALRNGDVEYPFRAS
                     SDFFLLTGMTEPESWAVLTKQGQKRSFQLYVRPFDPEQEIWTGRRAGLKGARKTFGAD
                     VSHDVAEFFNHLPEMLQNQPRLYYSFGIDDRNDARILAVTNSLKQEARRGILGPWEIH
                     DTRRITWRMRLTKDRLDILDQKKVACITAQAFKAAMRAVRPGLREYELASVVEFEFAR
                     RGGKRLAFDTICAAGANAATLHYTSNDAVIGPDDLVLVDAGAEHDMVTADITRTFPAS
                     GHFTKPQATAYQWVLKAQKAAIAKIRPGVTYAEIHRAGLEVLCAGLVAMKVLDAPIEQ
                     IIESQAYRPWFMHRIGHWLGLDCHDVGPYFIDGASIRLEPGMVITVEPGLYFRNQPEV
                     PEEYRGIGIRIEDDVLVTTNGSKILTSDVPKEIKDIEAFMSESASWWDGIEPVSTE"
BASE COUNT          814 a         1006 c         1018 g          712 t
ORIGIN      
        1 gaatccctgt gtgacttcat gaccaggccg gtaagccgtc caacggaatt ggcacccaca
       61 aggccggcct cggcaataat ctggcgaaca agctgcctgg tggcgacctc atccagtctt
      121 tccgggagcc agcccttcaa cagctcgatc tcgaaccgat atgactgaag tattggatta
      181 tcgccgtgcc cggccttttc gacttcctcg atcgtctttg ccagcttctt ggaatatgag
      241 gacatcacgc caagcaccag ctcatccgta acgccgccgg tgaatccggg ggccgtccgc
      301 ttttcggcca ccaggctctt gatcatcctg aggcatgcca gctccgactg gttccttgcc
      361 ctcatcgcat cctttatcgc atcgtcaagc ttctgttcaa ggctcatggc aaagcctcca
      421 tactgagtcg gcggcattat acacccgatg tcgtcggcga cacgcattca cgattgaaaa
      481 acagggtgct ctatgttact ttcccccacg tttttctgta cgcgtgtacg gaccgcgctt
      541 tcggtgcaat cccgggagcg atgttggagg taccaatgaa gctgatgagc ggcaaagtca
      601 ccccgctggc gtccgagctt gtggacgaac tggtcaagtc ggaaaacatt gaagtcctgc
      661 ccgaggaaat gcccgaagtc cagcttgatc tcgagtccgt tctgaaggaa tacatcagga
      721 ccgaccgcga aatcaccgaa aaggcgcggg acatgatcag ccgccagaag cgtgactacg
      781 gcgacctgcc gaaagtgaag gcgcaggtgg cgcgcgaaag ggatttcggc gtcggcgaga
      841 accttatcga atacatcacg gcgcagctta tcgaggcact gatccacagc cggcatgtcg
      901 aggaagtttt cggcatggac aatgacctta cgctggtgat gacgccgatt ctcaagaagc
      961 atctggccgc cgacgaggag cttgaccagg aagttcgcaa acgaatcaag aacctgcagg
     1021 aaggcacgat gacctgggac gcccaatacc agaagctgaa ggatgaactc aagcgcgtcc
     1081 gcaagctcga cgactgacca tatcccgcca cttttcgatt gccccgcaca acgaatcgaa
     1141 tcattatcat ccaggtgctc tgtcagggtt cgacatgagg attgaaggaa gcaggatcgc
     1201 cgttaccggc gcgaccggat tcatcggccg gcatatcgtt gaatcgctgc tggcagcggg
     1261 ggccgacgtt gtcgccgtcg tcaggaaccc ggacaaggtt cccgaattca ggcggatggg
     1321 cattgaattc aggatcgcgg atctcgggga caggattgcc ctgacccgtg ggtttcgcgg
     1381 cgtcgatgcc gtcatatcaa acgcggcact gttccgcatg agcaacagcg actgggacgg
     1441 acaccggcag gcaaacgttg ttggaaccgc gaacgtgatc agggcggtcg cggacgcagg
     1501 tgtcaccagg gttgttcatg tctcgtcggt agccgtttac aaatccgggg gcagcacgcc
     1561 gattggcgag gactggccga tgcttgaaga aagcgacctg ggcatgttca acgcatactc
     1621 cgtttcaaag gcagtttcag agcgtgaggc ctggaagctt gcatcaaaat tgggacttgc
     1681 cttgtcctgc tgcaggccaa gttcggtgtt cggcccgttc gacccgaaca tcaccccgac
     1741 catagcccgc gtgctttccc ttcccgttgc accgataccc caccttcttc gtgccggtct
     1801 tgtttatgcg ggcgacgtgg cggacggcac tttgaggatc cttcggaacg attcgacagt
     1861 cggcaagtct tacaacctga ccgggccaca ctctacatcg ggcgaattct ttcatgcctg
     1921 gaaggatgcc ggcgggccgt ggccaggcct ggcaatcccg gttccagtcc ccttgaccag
     1981 gatatggaac aaccagaggg ccgaaaggga acttggctgg aagaaccgtt cgtggcagga
     2041 atcactgtgt cagacattcg aaaggcagcc cttcgggcgc cgcaaaaagg cggaaggatg
     2101 atggacaaga cgcgattcag gaagcgaagg gaaacgtttt ttgacatgat ggaagacggg
     2161 gtggccctga taccgtccgg cagccccgcc ctgcgcaacg gcgatgtcga atatccgttc
     2221 agggcgtcaa gcgacttctt cctgctgacc ggaatgaccg aacccgagtc atgggcggtt
     2281 cttacgaaac agggccagaa gagatcattt caactgtacg taaggccttt cgatcccgag
     2341 caggaaatct ggaccggccg acgggccggc ctgaagggcg ccaggaagac tttcggcgcg
     2401 gatgtttccc acgatgtcgc cgaatttttc aaccacttgc cggaaatgct tcagaaccag
     2461 ccgcgccttt actattcgtt cggtatcgat gacaggaatg atgccagaat ccttgccgtc
     2521 accaacagcc ttaaacagga agccaggcgc ggaatcctgg gaccatggga aatacacgac
     2581 acacgcagga ttacctggcg gatgcgcctg accaaggatc gattggacat cctggaccag
     2641 aaaaaggttg cctgcatcac ggcccaggca ttcaaggcgg cgatgagggc ggtaaggccc
     2701 ggcttgcgcg aatatgaact ggcctcggtg gtcgaattcg aattcgccag acgcggtggt
     2761 aaacgacttg ccttcgatac catctgcgcg gccggagcaa atgccgctac ccttcattac
     2821 acatcaaacg acgccgtcat cggcccagac gatcttgttc ttgttgacgc cggggccgag
     2881 catgacatgg tcacggcaga catcacgcgc acattcccgg cgtccggcca tttcacaaag
     2941 ccgcaagcga cggcatacca gtgggttctg aaggcgcaga aggcggcaat cgcgaaaatc
     3001 cgtccgggtg tgacctatgc ggagatccac cgggccggac tcgaagttct ttgtgccgga
     3061 ctggtcgcca tgaaggttct tgacgctccg attgaacaga ttatcgaaag ccaggcttac
     3121 cgtccgtggt tcatgcaccg catcggccac tggctcggcc tggactgcca cgatgtcggc
     3181 ccgtacttca tcgatggcgc atcaatcagg ctggaacccg gcatggtcat caccgtggaa
     3241 cctggcctgt acttccgaaa tcaaccggaa gtccccgaag aatatcgtgg aatcggaatt
     3301 cgaatcgagg acgatgttct tgtaaccacg aacggctcaa agattctaac atcggacgtc
     3361 ccaaaggaaa ttaaagatat agaagcattc atgagcgaat ccgcaagctg gtgggacggg
     3421 atcgaacctg tatcgaccga ataaatcccg ttgacagacg catcgcttgc atctaaaatt
     3481 tttgcgcttt cgtgactggc gaagtagctc agttggtcag agcaggcggc tcatatccgc
     3541 cgtgtcgggg
//
LOCUS       DAOPPA010000347         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28604_length_3549_cov_3.648827, whole genome shotgun sequence.
ACCESSION   DAOPPA010000347 DAOPPA010000000
VERSION     DAOPPA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28604_length_3549_cov_3.648827"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2511)
                     /locus_tag="PKH54_10305"
     CDS             complement(118..2511)
                     /locus_tag="PKH54_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOD00330.1"
                     /translation="MDLITKLKGAWYLGGTIIFGSVKYAFRRASVERRFRRNTFNKPF
                     QIGGLHRTSELESGMMFEFENAFLEVVFVMPGVPRLTWTPGELPLNYAVSRSEFPPVQ
                     VRMAREDEYHVLESNGLIVTVKPDGSITFTHDGTLLRHDHPPRMMKAGWNTRTDMAAD
                     EAFFGMGMRSHGFNVRDGVFKMWNREPQGAYAPGDDPLYVSSPAYLSVSPRGSYLAFF
                     DNTFQSVFSFKGRIDCQFTGGAVRYFMFAGTPEENVMRFYDLVGHPAMPPRWALGFHQ
                     CRWSYMSDAEVRQIASGFENNDMPVSAIHMDIHYMDGYRVFTVDKARFPDLGALAAEM
                     DGKGIRLVTILDPGVKIDDQYPLYREGCEKDLFLRVPDGSHVESLVWPGWCAFPDFSS
                     PAAREWWADQYKFLLDQGISGFWHDMNEPAGFAAWGDHTLPTAAVHSLEGRGGSHLEA
                     HNVYALGEAMAGFDAQKKFRPERRPWILSRSGWPGLARYAWHWTGDSESNWWSLAQTL
                     RIAMNLAVSGIPYTGADIGGFGGRPDRELYLRWFQACSVLPFFRVHSAFFTPRREPWC
                     FDDEVTRITREHLRRRYLLMPVWYSLAAAATRTGRTPIRPMFMEDVSMVSVDDQFMVG
                     RALLAAPVVEKGARDRCIRFPEGNWYDFETGEFIEGGKRRCVKAPLDFMPLYAREGSI
                     VPVDPSAGPAEGDYARIELRVFMPNADGIFPGGELFRDAGDGYGPWLLERFELASEEA
                     TTTIFRHASGDFKPACDHFVIRTIGDDETAEIKVDGVAVERTDGVFVVPATFEKIEI"
     gene            2692..>3549
                     /locus_tag="PKH54_10310"
     CDS             2692..>3549
                     /locus_tag="PKH54_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00331.1"
                     /translation="MFFAKPTAFVTVLLALSIIIPLRLAASESEVAPAAPDSEVVPVV
                     ASDGDGVPVDSVAAEPVQAVSAEDDSAGDVNPPEEGKDDVAAAPADESVSEDADKGSK
                     KSASKSKKKGDKSEKSAAKGKKKGSDDEDDKKKDPNRVEWLPFPVVGGNTDMGILLGA
                     QLMLAKFKEGYTPYRFRTHTQISLSFKGGEKGVQIPVHMDFVKLDFPGLAGGKLRLGL
                     GFHYDQVNNSGYFGVGNKAPYLRSWWDSELNRTVQPVSRTYQYMRQEPKLRVSFRYHL
                     QKNLELALGL"
BASE COUNT          722 a         1074 c         1042 g          711 t
ORIGIN      
        1 tctgtgcgtt gccccgggcg aattttcatt cgcccgtacg ggcggattaa tggattacat
       61 ccgcccgccg cggttctgca agatgtgcaa gccgatcact cccgcacgga attccgccta
      121 gatttcaatt ttttcaaacg ttgccggcac cacgaacacc ccgtccgtgc gttccaccgc
      181 tacgccgtcg accttgattt ccgcggtctc gtcgtcgccg atggtgcgga tcacgaagtg
      241 atcgcaggcc ggtttgaagt cgcctgaagc gtgcctgaaa atcgtcgtgg tggcttcctc
      301 gctggcaagt tcgaaccgtt ccagcagcca gggcccgtaa ccgtccccgg cgtcgcggaa
      361 aagttcgccc ccggggaata tgccgtccgc gttaggcatg aacacccgaa gctcaatccg
      421 cgcgtagtcg ccctctgccg ggccggcgga cggatcaacc gggacgatgg agccctcgcg
      481 ggcatacaga ggcatgaagt ccagcggagc cttgacgcac cgcctcttcc cgccctcaat
      541 gaattcaccc gtttcgaagt cgtaccagtt gccttcgggg aacctgatgc acctgtccct
      601 ggcacccttc tcgacgaccg gggcggccag aagggcgcgg ccgaccatga actggtcgtc
      661 gacgctgacc atgctgacgt cttccatgaa catggggcgg atcggcgtgc gtccggttct
      721 tgtcgcggcg gccgcaagcg aataccaaac cggcatcagc aggtatctgc gccgcaggtg
      781 ttccctggtg attcgtgtca cctcgtcgtc gaagcaccac ggttcgcgtc gcggggtgaa
      841 gaacgccgag tggacccgga agaagggaag caccgagcac gcctggaacc atcgcaggta
      901 aagttccctg tcgggtcgtc cgccgaaccc gccgatgtcc gcgcctgtat agggaattcc
      961 cgacacggcc aggttcatcg caatccttaa tgtctgtgcc aggctccacc agttgctttc
     1021 gctgtcgccg gtccagtgcc atgcgtaccg ggccagtcca ggccatccgg atcttgacag
     1081 aatccagggg cgcctttccg ggcggaattt cttctgcgcg tcgaagcccg ccatcgcctc
     1141 gccaagcgca tagacgttat gggcctcaag gtggcttcca ccgcgtcctt caaggctatg
     1201 gaccgcggct gtcggaagtg tgtggtcgcc ccacgccgca aaaccggcag gctcgttcat
     1261 gtcgtgccag aaaccggaaa tgccctggtc aagcaggaac ttgtactgat cggcccacca
     1321 ttcgcgggcc gcggggctgg aaaagtcggg gaatgcgcac cagcccggcc agaccagcga
     1381 ttcaacatgc gaaccgtccg gaacccgcag gaaaagatcc ttttcgcatc cttcgcggta
     1441 aagcggatac tggtcatcaa tcttgacccc gggatcaagg atcgtgacca gccggattcc
     1501 ctttccgtcc atctccgcgg ccaacgcgcc aagatccggg aacctggctt tgtcaacggt
     1561 gaagacccgg taaccgtcca tgtaatggat atccatgtgg attgcggaaa ccggcatgtc
     1621 gttgttttca aaaccactgg ctatctggcg aacctcggcg tcgctcatgt acgaccagcg
     1681 gcactggtgg aatcccagag cccatcgcgg cggcatcgct ggatggccga ccaggtcgta
     1741 aaagcgcatc acgttctcct cgggtgttcc ggcgaacatg aaatacctta cggcgccgcc
     1801 cgtgaactgg cagtcaatcc ttcccttgaa cgaaaagacg ctctggaagg tgttgtcgaa
     1861 gaaggccagg tatgaacccc gcggagacac cgaaaggtag gccggcgacg acacgtaaag
     1921 aggatcgtcg cccggcgcgt aggcgccctg gggctcgcgg ttccacatct tgaagacgcc
     1981 gtcacggacg ttgaagccat gcgatctcat gcccatgccg aaaaaggcct cgtccgccgc
     2041 catgtcggtt ctggtgttcc agcccgcctt catcatccgc gggggatggt cgtgtcgcag
     2101 cagcgttccg tcgtgcgtga acgtaatgct tccgtccggc ttgaccgtga cgatgagtcc
     2161 gttgctttcc agcacatggt attcgtcttc cctggccatt cgaacctgca ccggcggaaa
     2221 ttcgctgcgt gaaaccgcgt agttcagcgg aagctcgccg ggggtccagg tcagccgggg
     2281 cacgccgggc attacgaaga ccacctcgag aaaggcgttt tcaaattcga acatcatccc
     2341 tgattccagt tccgacgttc tgtgcagccc cccgatctgg aacggcttgt tgaatgtgtt
     2401 cctgcgaaac ctgcgttcga ccgacgcacg cctgaaggcg tacttgaccg agccgaagat
     2461 gatggttcca ccaagatacc aggcaccctt caacttggtt atcaagtcca tctgaacccc
     2521 caaaatgccg taacgcgctg catcaagaaa tgcgtccgca gccgggaacc gcgtcccgtc
     2581 tgtccagata cgccattatt aacatcaatc gcgtccgccc ggccctgaaa tgttttgcgc
     2641 gaaggtgctt ttttcccttg acgcaaaacg atttgcaact aggcttttgc catgtttttc
     2701 gcgaagccga cagccttcgt caccgtcctg cttgccttga gcattattat acccctgcgt
     2761 cttgccgcat ccgagtccga agttgcgccg gcggcccccg attctgaagt tgtgcctgtc
     2821 gtggcgtccg atggcgatgg tgtcccggtc gattcggtgg cggctgaacc cgttcaggcc
     2881 gtttcggcgg aagatgattc ggcaggggac gtgaatccgc cggaagaagg caaggacgat
     2941 gtcgcggcgg ccccggccga cgaaagcgtg tcagaggatg ccgacaaggg ctcgaagaag
     3001 tcggcctcga agtcgaagaa gaagggcgac aagtcagaga agtcggcggc caagggaaag
     3061 aagaaaggct ccgatgacga ggatgacaag aaaaaggatc cgaaccgtgt cgaatggctg
     3121 cccttcccgg ttgttggcgg caatacggac atgggaatcc tgcttggcgc gcagttgatg
     3181 ctggccaagt tcaaggaagg ttacaccccg taccgcttca ggacgcacac gcagatttcc
     3241 ctgtcgttca agggcgggga gaagggcgtt caaataccgg ttcacatgga tttcgtgaag
     3301 ctggactttc ccggactggc cggcggaaag ctgcgcctgg gactgggttt tcattacgac
     3361 caggtcaaca attccgggta tttcggtgtt ggcaacaagg cgccatacct gcgaagctgg
     3421 tgggattccg aacttaacag gacggtgcag ccagtttcaa gaacgtacca gtacatgcgc
     3481 caggaaccga agctgcgggt ttcattccgt tatcatctgc agaagaatct ggaactcgca
     3541 cttggcctg
//
LOCUS       DAOPPA010000348         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28631_length_3547_cov_3.828751, whole genome shotgun sequence.
ACCESSION   DAOPPA010000348 DAOPPA010000000
VERSION     DAOPPA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28631_length_3547_cov_3.828751"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1149
                     /locus_tag="PKH54_10315"
     CDS             223..1149
                     /locus_tag="PKH54_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOD00332.1"
                     /translation="MPVMMETTPRQAADEFLAHIGIAKSASPNTIKGYGIDIVEFFQI
                     CDDAGLDPFKSGRDLVAAWFQAVHDRHAPSSITRKLAAIRGMYKYWKLRGWVPSNPFS
                     GVRGPKLPQTLPDFLPVDQAFRLIDGIQGDDPAACRDRAILELLYGGGLRVSELSGLD
                     IRDVNLRSGEARVLGKGRKERIVPLGSKAVSAVDSYLAVRARVPSEKSGVVDAAALLL
                     NRFGTRLTVRGIARVIDKAVERVALARNVHPHTLRHTFATHLLEGGADLRDIQELLGH
                     SRLSTTQKYTHVTLARLQDVYDRAHPRARSDD"
     gene            1507..2055
                     /gene="hslV"
                     /locus_tag="PKH54_10320"
     CDS             1507..2055
                     /gene="hslV"
                     /locus_tag="PKH54_10320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOD00333.1"
                     /translation="MQEQQGSPIRGTTVICVRKGGRVVIAGDGQVTYGNTVLKGSAVK
                     VRRAADGRVLVGFAGSTADAFTLFEKFEGKLKDFNNNLARAALELAKEWRMDKALRRL
                     DALLIVADREKTLLLTGNGDVVEPEDGIVAIGSGGPYAQAAARALTMKTSLDAAEIAA
                     TAMQVAAGMCIYTNDRITVEEL"
     gene            2055..3398
                     /gene="hslU"
                     /locus_tag="PKH54_10325"
     CDS             2055..3398
                     /gene="hslU"
                     /locus_tag="PKH54_10325"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOD00334.1"
                     /translation="MSKEIQAPAAREFTPREVVAMLDRYIIGQSEAKKMVAIALRNRW
                     RRMQVPEPLRDDITPKNILMIGPTGVGKTEIARRLAKLSGAPFIKVEASKYTEVGYVG
                     RDVESMVRDLVEVAVKIVRQAEGERVAAKAEVRAREKVADTLVRQAPVNAPLDRQTLL
                     QAIADKDPATMKMTLRIEIPKPQFPGVSIFAGGGMDDIGAQVQEMLGNFMPGKPKDRT
                     VTVSDALRIFDESEREQLMDPEKIKEQACALAEHSGIIFIDEMDKIAGRNSGGHGPDV
                     SREGVQRDILPIVEGTQVSTKYGLVKTDHILFIGAGAFHISKVSDLIPELQGRFPIRV
                     ELDSLTGEDFRRILVEPDNSLVKQYVALLAAEGVILEFRDDGIRRIAEIAADLNGRLE
                     NIGARRLHTVLEKVLEEVSFMAPEIQGGVVPITADYVSQRLEKFAESEDLARYIL"
BASE COUNT          720 a         1051 c         1136 g          640 t
ORIGIN      
        1 agcaccgtcc gcctgtccgg aggcatcttc gcgggccgaa ccggcgttgt ccaggacatg
       61 gatgccaagg gtaacgtcaa ggtcctggtt ggccgagtca ccgtccgcac cgaatcccac
      121 atggtccgcg ccgtctgatt tcggcttctg gatgacgacc gaatccgccc gtgtaatcaa
      181 ggtttccagg ccgccgggac aagatgtgtc tgccggttta taatgcctgt catgatggag
      241 acgacaccac gacaggcggc ggacgagttc ctggcgcaca tcggcattgc gaaaagcgcg
      301 tcgccgaaca ccatcaaggg atacggaatc gacatcgtcg aattcttcca gatttgtgac
      361 gatgcgggtc tggatccgtt caagtccggt cgcgacctgg tcgcggcatg gttccaggcg
      421 gttcacgacc ggcacgcgcc gtcctccatt acccgcaagc tggccgcaat ccgcgggatg
      481 tacaagtact ggaagctgcg cgggtgggtg ccttccaatc cgttttccgg cgtgcgcggg
      541 ccgaaactgc cccagacgtt gccggatttc ctgcctgttg accaggcgtt caggcttatc
      601 gacggcatcc agggtgacga cccggcggcg tgtcgcgacc gcgccatcct cgagcttctt
      661 tacggcggcg ggctgcgcgt gtcggaactt tccggccttg acatacgcga cgtcaacctt
      721 cgatccggcg aggcaagggt cctggggaag ggccgcaagg aaaggattgt cccgctcggg
      781 tcgaaagccg tttccgccgt tgactcgtat cttgcagtcc gcgcccgcgt cccgtccgaa
      841 aagtccgggg tcgtggatgc tgcggcgctg ctgttaaacc ggttcggcac gcggttgacg
      901 gtccgcggca tcgccagggt catcgacaag gctgtcgaac gtgtcgcgct ggccaggaac
      961 gtgcatcccc acacgctgcg ccatacgttc gcgacccatc tcctggaagg tggcgccgac
     1021 ctgcgtgaca tccaggaact gcttggtcat tcgcgccttt caacgacgca gaagtacacg
     1081 cacgtcacgc tggcccgcct gcaggacgtg tacgaccgag cccacccccg cgccaggtca
     1141 gacgactgac gaattcgaat gacgtgcaga cgccggcgac agcgcccgcg caaagtgcca
     1201 tttcgtctgc gaaaacggcc tggaggccgg cgagaacggc cttgccggaa gctggtgcga
     1261 tgtcgcctgc gattcatgca tttcgattca tggacttacc tgcgataact gcaatttgct
     1321 ttccgaggat gaagggactc cacagtgccc ctgaacagga cggcatttcc ggcgtcgcat
     1381 cagatccggc tgcaataatt ccgaataaat cgtaaccggg gcttgcccaa tgggcagttc
     1441 ggcacaagat taactggcag tgattcttcc gggccagatc gaccaggtca gtttttgggg
     1501 gttacgatgc aggaacagca gggaagtccc atcaggggaa ccaccgtcat atgcgtcagg
     1561 aagggcggcc gcgtcgtcat cgccggcgac gggcaggtca cgtacggcaa caccgtgctg
     1621 aagggttccg cggttaaggt tcgccgtgcc gcggacggcc gtgtcctggt cggcttcgcc
     1681 ggatcgaccg ccgatgcgtt cacgctgttt gaaaagttcg aaggcaagct gaaggacttc
     1741 aacaacaacc tggccagggc cgcgctggaa ctggccaagg aatggcgcat ggacaaggcg
     1801 cttcgtcggc ttgacgcgct tctgatcgtg gccgaccgtg aaaagaccct gctgttgacc
     1861 ggcaacggtg acgtggtcga gccggaagac gggatcgtcg cgatcggatc gggcggtcca
     1921 tatgcccagg cggccgcgcg ggccctgacc atgaagacgt cgctggacgc cgcggaaatc
     1981 gcggcgacgg caatgcaggt ggcggccggg atgtgcattt acacgaacga ccgcataaca
     2041 gtcgaggaat tgtgatgtcg aaggaaatcc aggcgccggc agcgcgcgaa ttcacgccgc
     2101 gcgaagtggt ggccatgctt gatcgctata tcatcggcca gtcggaagcc aagaagatgg
     2161 tcgccatcgc actgcgcaac cggtggcgcc ggatgcaggt gcccgagccg cttcgtgacg
     2221 acataacgcc gaaaaacatc ctgatgatcg gtccgaccgg cgtcggcaag accgaaatcg
     2281 cccgacggct ggccaagctg tccggcgcgc ccttcatcaa ggtcgaggcg tccaaatata
     2341 ccgaggtcgg ttatgtggga cgcgacgtcg aaagcatggt tcgcgacctt gtcgaggtcg
     2401 cggtgaagat cgtgcgccag gccgagggcg aacgcgtcgc ggccaaggct gaagtcaggg
     2461 cgcgcgagaa ggttgccgac accctggtgc gccaggctcc ggtaaacgcg ccgcttgacc
     2521 gccagacact gcttcaggcc atcgcggaca aggatccggc gacgatgaag atgacactgc
     2581 ggatcgagat cccgaagccg cagtttcccg gcgtctcgat ctttgccggc ggcggcatgg
     2641 acgatatcgg cgcgcaggtc caggaaatgc ttggcaactt catgccgggc aaaccgaagg
     2701 atcgaactgt caccgtttcc gacgcgcttc ggatctttga cgaatccgag cgcgagcaac
     2761 tgatggaccc cgaaaagata aaagaacagg cgtgcgcgct tgcggaacat tccgggatca
     2821 tcttcatcga cgagatggac aagattgccg gccgcaacag tggtggacac ggcccggacg
     2881 tttcccgcga gggcgtccag cgtgacatcc tgccgatcgt cgagggtacc caggtcagca
     2941 cgaagtacgg gttggtcaag acggaccaca tccttttcat cggcgccggg gcgttccaca
     3001 tcagcaaggt gtccgacctg attcccgagc tgcagggacg cttcccgata cgcgttgaac
     3061 tggacagcct gaccggcgag gacttccggc gcatcctggt cgagccggac aacagcctgg
     3121 tcaagcagta cgttgccttg ctggccgccg agggcgttat ccttgagttc cgcgatgacg
     3181 gcatccgtcg cattgccgag atcgcggccg acctgaacgg gcgcctggaa aatattggcg
     3241 cgcgccggct gcacacggtt ctggaaaagg tactcgagga agtctcgttc atggcgcccg
     3301 agatccaggg cggggttgtc ccgataacgg ccgattatgt ctcgcagcgg cttgaaaaat
     3361 tcgccgaatc ggaggatctt gccaggtaca tactttgaat tgattgcggc catcgggccg
     3421 actggagatt gaaatgccac cagagcgcaa gaaatcgata ccggtaaaga tgaaggacga
     3481 ggtcatcggc ggcgtctata cgaacaacat gatggtcgcg cacacgcgcg aggagttcgt
     3541 gatggac
//
LOCUS       DAOPPA010000349         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28751_length_3533_cov_3.779183, whole genome shotgun sequence.
ACCESSION   DAOPPA010000349 DAOPPA010000000
VERSION     DAOPPA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28751_length_3533_cov_3.779183"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1479
                     /locus_tag="PKH54_10330"
     CDS             85..1479
                     /locus_tag="PKH54_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD00335.1"
                     /translation="MMARILVVEDDQSLREVLRMFLNKSGHDVTVCGEGDKALKLIAS
                     EDEFDLVLTDLRLGAVTGLEILTFVKEHSPQTEVIMMTAFSTVETAIDAMRRGAFDYI
                     GKPFKLEEISITLQKALERRNLSLENIRLRIALSDKYSFAHIVGKSQTMHKVFEKIIK
                     VAPTKTSVLITGESGTGKELVARAVHFNSNRKDKPFVVVNCGAIPDQLMESELFGHVK
                     GAFTGAHATRHGLFESADGGTIFLDEIGELSLAMQVKLLRFLQDHKIRMVGGVQEISV
                     DTRVVAATNRDLSQLVQAGAFREDLFYRLNVIGLHLPPLRERREDIPVLVMHFIEKYR
                     KELGRDDIRGVSPEVMDILSAHEFKGNVRELENIVEHAMTFASDDRITPDALPEGLRT
                     SSRSDRSQQDSIFRVPEDGMDLEALLADIERRLLLDALSRCGGVRKNAADLLGISFRS
                     IRYKLAKYGLGDEE"
     gene            1550..1622
                     /locus_tag="PKH54_10335"
     tRNA            1550..1622
                     /locus_tag="PKH54_10335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1583..1585,aa:Lys,seq:ttt)
     gene            1635..1707
                     /locus_tag="PKH54_10340"
     tRNA            1635..1707
                     /locus_tag="PKH54_10340"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1669..1671,aa:Glu,seq:ttc)
     gene            complement(1826..3148)
                     /locus_tag="PKH54_10345"
     CDS             complement(1826..3148)
                     /locus_tag="PKH54_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOD00336.1"
                     /translation="MDGSGGGQGKGGDRGIGSRRATRLIHGRSQSSMWEYSHHAIPPI
                     TASTTFRLSSVSRGQKGFLAFGSPDLGHGESPTYIYDRLDEPTTSMLEEVLADGEGGD
                     RAVAFACGMSAISSTLMACCSVGQSIVAHPVMYGCTYSLLTKQLPRFGITTVFADMRD
                     PEAIRLAITAHTRVVYFETPANPTLECIDIQNVATVVGAINAARPVEQRVRIVVDNTF
                     QTLWGQRPIESGADIVVASLTKNVGGFGVDMGGVAVVPRELCGPLKGIRKDFGGVLPP
                     ASAWDFQVYGLSTLPVRLARQVQTSRAVAEFLASHPAVSRVSYPGLPDYEFREIAEKQ
                     MRTPEGEFCPGHMIYFELAGDPEISANRRERFINYLAEHAYSIMLAVSLGMTKTLIEA
                     PGLMTHSSFDPETQMKLGIHPGGIRLAIGLEDARDVISDLSEALENSI"
BASE COUNT          769 a         1052 c          988 g          724 t
ORIGIN      
        1 ccttgccaag gttgacgtcg cgggttcgaa tcccgtttcc cgctccaaac cacacaaacc
       61 gtcgcatgac acgcccctgg aggaatgatg gcccgcattc tggtagtcga ggacgaccag
      121 tcgctgcgcg aagtcctgcg gatgttcctg aacaagagcg gtcacgacgt gacggtctgc
      181 ggggaaggcg acaaggccct gaaactgatc gcatccgagg atgaattcga cctggttctg
      241 accgatctgc gccttggtgc ggtgaccgga ctcgaaatcc ttacgttcgt gaaggaacat
      301 tcgccccaga ccgaagtcat catgatgacg gccttctcga cagtcgaaac cgcaatcgac
      361 gccatgcgca ggggcgcctt cgactacatc ggcaagccgt tcaagctcga agaaatcagc
      421 atcaccctgc agaaagccct ggagcgacgc aatctgtcgc tggaaaacat ccggctgcgg
      481 atcgcgctgt ctgacaagta cagcttcgcc cacatcgtcg gcaagtccca gacgatgcac
      541 aaggtattcg agaaaatcat caaggtcgcg ccgaccaaga ccagcgtcct gattaccggt
      601 gaatccggaa ccggcaagga actggtcgcg cgcgccgttc acttcaactc gaaccggaag
      661 gacaagccgt tcgtggttgt gaactgcggc gcgattcccg accagttgat ggaatccgag
      721 ctcttcgggc acgtcaaggg cgcgtttacc ggtgcccacg cgaccagaca cggcctgttc
      781 gagtcggccg acggcggaac catattcctt gacgaaatcg gcgaattgtc cctggccatg
      841 caggtgaagc tccttcgatt cctgcaggat cacaagatcc gaatggtcgg gggggtgcag
      901 gaaatctccg tcgataccag ggtcgtcgct gccaccaacc gtgatctttc ccaacttgtg
      961 caggctggcg ccttccgcga agacctgttc taccggctta acgtcatcgg cctccacctt
     1021 ccgccccttc gggagcgacg cgaggatatc ccggttcttg tcatgcactt catcgagaag
     1081 taccgcaagg agcttggacg ggacgatatc aggggtgtct cgccggaagt catggacatc
     1141 ctttccgccc acgaattcaa gggcaacgtc cgcgaacttg aaaacatcgt cgagcatgcg
     1201 atgacgttcg cctctgacga caggatcacc cccgacgcgc ttccggaagg cctccgaaca
     1261 tcatccaggt cggacaggag tcagcaggat tccatcttca gggttcccga ggacggaatg
     1321 gatctggaag cgctgctggc cgacattgaa aggcggctgc tgctcgacgc gctgtcccga
     1381 tgtggcgggg tgcgcaagaa tgctgccgac cttctgggca tcagcttcag gtcgatcagg
     1441 tacaaactgg ctaaatacgg acttggcgac gaagaataat tcgttgttgc agaaaaattc
     1501 cattgacaca attcgaccca gcaaatacca tatcccgcgt ttcgttgctg ggcccatagc
     1561 tcagccggta gagcagcggc cttttaagcc gttggtcgcg agttcaaatc tcgctgggct
     1621 cacttttttt ccgtgacccc atcgtctagt ggtctaggac acgggccttt cacgcccgcg
     1681 acacgggttc gactcccgtt ggggtcgctt aagcgacaat ttccgcgttt gtgggtgcct
     1741 tcatgccggg cgccaggccg tttcggttcc ccgaccgcat cttcactgga ttcgtcggta
     1801 tgttcaacgc ctgaacggca atgcttcaaa ttgaattctc gagggcctct gaaagatccg
     1861 aaatcacgtc gcgggcgtct tccagtccga ttgccagcct gattccgccg ggatgaatgc
     1921 caagcttcat ctgtgtttct ggatcaaacg acgagtgcgt catcagtccg ggggcctcga
     1981 tcagggtctt ggtcatgccg agactgaccg ccagcataat cgagtaggcg tgctcggcaa
     2041 ggtagttgat gaacctttcc cgacgattcg cggagatttc gggatcgccg gccagctcga
     2101 aataaatcat gtgaccggga cagaactcgc cctcgggcgt ccgcatctgc ttttccgcga
     2161 tttccctgaa ttcataatca ggaagaccgg gataggaaac cctgctgaca gccgggtgcg
     2221 acgcaaggaa ttcggccacg gcccgggacg tctgcacctg cctggcaagg cggacgggaa
     2281 gggtcgaaag cccgtaaacc tggaaatccc aggcggacgc cggtggaagt actccgccaa
     2341 aatccttgcg aatccccttc agtggcccgc aaagttcccg gggaacgacc gcaacgccac
     2401 ccatatcgac gccgaagccc ccgacgttct tggtaaggct tgccaccacg atatcggcgc
     2461 cggactcgat tggacgctgt ccccacagcg tctggaaggt gttgtcaacg acaatcctga
     2521 ccctctgttc caccggacgg gcggcgttta tggcgccgac caccgtggcg acgttctgga
     2581 tgtcgatgca ctcaagcgtc ggattggccg gggtctcgaa atacacgacc cttgtatggg
     2641 ccgtaatcgc cagtcggatc gcttcaggat ccctcatgtc ggcaaaaacc gtcgtgattc
     2701 cgaacctggg gagttgtttg gtcaacaggc tgtaagtaca cccatacatg acggggtgtg
     2761 ccacgattga ctgtccgacg gaacaacagg ccatcagagt cgaggagatc gccgacatgc
     2821 cgcaggcgaa cgcgacggcg cgatccccgc cttccccgtc ggccagcact tcttccagca
     2881 tcgatgtggt cggttcgtca agcctgtcat agatgtaggt cggactttcg ccatgtccca
     2941 gatccgggct gccgaaagca aggaaaccct tctgcccgcg ggaaaccgaa gaaagcctga
     3001 aggtcgtgga tgcggtgatc ggtggaatcg catgatgact gtattcccac atgctcgact
     3061 gcgaacgacc atggatgagc ctggttgcgc gcctgctgcc gatgcccctg tccccgcctt
     3121 ttccctggcc gccgccggaa ccgtccatgt gaacctccag atgacttcac acccccgcga
     3181 aatcaacggc aacgcgcctt gatcggcagg gggctcccgc ccgaagaata gtccggaata
     3241 caacccacag tcaatcgacc ttcacccttg ttatgcaaat gtaaacaaac cgtcaatcag
     3301 accggacggt aaacagttgg ccgcctgtga tactgttgtc aatccaagca gttgtaaaat
     3361 tgacatatac agacatcaac ggtcagccaa tagaccgaag ttccggagcg atttgagcgt
     3421 tatcttcccg taatacttca tttatacgtc aaatgcagtg tcgggtttct gactacatgc
     3481 cgagcaattc gaatgctttc tgctgcaccg gtgttggctg ggagtactgg tcg
//
LOCUS       DAOPPA010000350         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28761_length_3532_cov_3.347714, whole genome shotgun sequence.
ACCESSION   DAOPPA010000350 DAOPPA010000000
VERSION     DAOPPA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28761_length_3532_cov_3.347714"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKH54_10350"
     CDS             complement(<1..575)
                     /locus_tag="PKH54_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00337.1"
                     /translation="MVKRRPAFCLGPFLTLAVLTFFLHACGSGASGPDSGNVVDSGSD
                     VAMDAGHDLPQADLPPSDSIDASAADVPADAPVDGETPDSGDIIAGPDDSSDPIDSSV
                     VDLNEDDSDVADPACPVFDDPVVLSNMEGTVLTELSGLAASRIHEGVLWAHNDSGDSA
                     RIFAVVPDPSQSSGPPLITPFAFDSEGQTWG"
     gene            complement(575..1489)
                     /locus_tag="PKH54_10355"
     CDS             complement(575..1489)
                     /locus_tag="PKH54_10355"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOD00338.1"
                     /translation="MGQRIGSLFARFFVAAACLLAVNMAGCSHPQVKADGTPEAVGID
                     EFLSQGKRTMWIAAHPDDECFAGSILARSSIHYGNPLHMVVMTRGDGGECGLPGGCHP
                     DTATVRTAEMQKVVEIYKSGLSQESFFNAPLPVESFPKRHELYERWKEHRDPVEYLVQ
                     EIRKFKPDVVFTFEPTRGGTGHPEHQLVSRLATTAIRQAADPAVSGGEPHKVERTYFM
                     LNRFWLFRMLGAADPGPVSEVFDATLPATGKLSCVDFMAHATTFHKTQANDMGTVRKG
                     RIMFNNLPLRRVDPFTQSWDPAEKEGGK"
     gene            complement(1522..2118)
                     /locus_tag="PKH54_10360"
     CDS             complement(1522..2118)
                     /locus_tag="PKH54_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOD00339.1"
                     /translation="MLAGSCQMSPVTILLIAVGLAMDAFAVSLATGCTMGVRVRPAHV
                     ARMAGAFGGFQALMPLLGWLGGKGLAGFLAPFDHWVALGLLGFIGVRMVVGGIRNNAC
                     EAPTDRDPTRGLVLLGLALATSLDALAVGLTLGVTGTGILVPCIVIGVVTFVMSAIGL
                     VSGKRLGCTFGSRMEIVGGLILVAIGVKIAVEHVVKAL"
     gene            complement(2298..3065)
                     /locus_tag="PKH54_10365"
     CDS             complement(2298..3065)
                     /locus_tag="PKH54_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00340.1"
                     /translation="MKPGRFLQFDIRPVFIPALVMACGLVACSSSLGDIPVDLPAEGV
                     SEPVACSPQGDFRFVIGCPRTPYVTGVTALGAHEALESVATAALGYDCLWVGRIVGIG
                     IGRDGKSTGAPLSGWTISWVGGEPEDPDMLVLDTTAGLCRAQNRCSCVSSGTCPDFSP
                     QNVAAAVMPAQDSPAAILAAFPDDPAGSMYDLVYDGKGQGWTVTPAATMPEQVDVEAP
                     DAAPDATPDAPLPDATPDAAVTDADPGDSVVQDGFDI"
     gene            complement(3115..>3532)
                     /locus_tag="PKH54_10370"
     CDS             complement(3115..>3532)
                     /locus_tag="PKH54_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD00341.1"
                     /translation="HLCGPARVIGLRDAVEINADVLRQHCPERGERLLLKTANSVGEW
                     WRQPFRPDYCHLTRDGADYLAKCGVRTVGIDYLSIGPGGREGDLVHRALLPAGVAIIE
                     GLVLGDVPWGRYEMLGLPLRIDGGDGSPARILLRPL"
BASE COUNT          721 a         1131 c         1099 g          581 t
ORIGIN      
        1 catccccatg tctgcccttc cgaatcgaaa gcaaacggcg tgatcagcgg cgggccggac
       61 gattgggacg ggtcgggcac tacggcgaaa attcttgccg aatcgccgga atcgttatgc
      121 gcccacagca cgccttcatg aattcgactg gcggcaaggc cggataattc ggtgagcacc
      181 gtgccttcca tattcgacag gactaccgga tcgtcaaaca ccgggcaggc cgggtcggcg
      241 acgtcggaat catcttcgtt caaatcgacg acggaagaat cgatcgggtc gcttgaatcg
      301 tccgggccgg cgatgatgtc accggagtcc ggcgtttcgc catcgaccgg cgcgtcggca
      361 gggacatcgg cagcagaggc gtcaatcgaa tcggacggcg gcagatcggc ctgcggcaga
      421 tcgtggccgg cgtccatggc aacgtcggag ccggaatcga cgacgttccc ggaatcgggc
      481 cccgaagcgc cggatccgca tgcatgaaga aaaaatgtca ggacagccag ggtcaggaat
      541 ggcccgagac aaaaagccgg cctgcgcttg accatcactt gccgccttcc ttttcggccg
      601 gatcccacga ctgggtgaac gggtcaacac gccgcagcgg caggttgttg aacatgatcc
      661 ggcccttgcg gaccgtgccc atgtcgtttg cctgcgtctt gtggaacgtg gtcgcgtggg
      721 ccatgaaatc gacgcaggaa agcttgcccg tggcgggcag cgtcgcgtcg aagacctcgg
      781 ataccgggcc gggatcggcc gcgcccagca ttcggaaaag ccagaagcgg ttcagcatga
      841 agtacgtccg ctcgaccttg tgcggttcgc cgccggaaac ggccgggtcg gcggcctgcc
      901 tgatggcggt cgtcgccagc cgggaaacga gctggtgttc cggatgaccg gtgccacccc
      961 tggtcggctc gaatgtgaac accacgtcag gcttgaactt gcggatttcc tgcacaaggt
     1021 actcgaccgg gtcgcgatgt tccttccagc gttcgtaaag ctcgtgccgc ttcggaaagg
     1081 actcgaccgg cagcggggcg ttgaagaagc tttcctggct gagtccggat ttgtaaatct
     1141 cgacgacctt ctgcatctcg gcggtccgga ccgtggcggt gtcgggatgg cacccgccgg
     1201 gaagaccgca ttcaccgccg tcgccacgcg tcatcacgac catgtgcagc ggattgccgt
     1261 aatgaatcga cgacctggca aggatcgaac cggcgaagca ctcgtcgtcg ggatgcgccg
     1321 caatccacat cgtacgcttt ccctgtgaca ggaactcgtc aatcccgacc gcctcgggcg
     1381 tcccgtcggc cttgacctgc gggtggctgc atccggccat gttcaccgcc agcagacacg
     1441 cggcggccac aaagaacctt gcaaataacg atccgatacg ttgacccatc ccgctgcctc
     1501 cgaaaccggc ccggccggtt gctataatgc cttgacgaca tgttccacgg caatcttgac
     1561 accgattgcc acaaggatca ggccgccgac gatttccatc cggctgccga aagtgcatcc
     1621 caggcgcttt cccgacacca ggccgattgc cgacatgacg aaggtcacca cgccgatgac
     1681 gatgcacggc acaagaatcc cggtacctgt cacccccagc gtcagaccga cggcaagggc
     1741 gtcaaggctg gtcgccaggg caagacccag caagacaagg cccctggtcg ggtcacgatc
     1801 cgtgggggct tcgcacgcgt tgttcctgat gccgccgaca accatcctta ccccgatgaa
     1861 gccaagcagg ccaagcgcga cccagtgatc aaacggcgca aggaatcccg ccaggccctt
     1921 gccgccaagc catccgagca gcggcatcag ggcctggaag ccgccgaaag cgccggccat
     1981 cctggccacg tgcgccggac gaacccgcac gcccatcgtg catccggtcg ccagggacac
     2041 ggcgaacgca tccatcgcga gaccgacggc aatcaggagt atggtcacgg gcgacatctg
     2101 gcatgaacct gcaagcatgc ggtcattccg gcagccagat tgtcgtttgc gcgggccgga
     2161 aggtcaaggc cggcggtttt gccttcgcca gggctacggc gttacagatt cgcagcggac
     2221 ggatgaaatg tactcgttcg cggcgggcgg atgtaatccg cccgtacaag gaaaacgccg
     2281 gccttggcgg aatcggacta aatatcgaat ccatcctgga cgaccgaatc gcccggatcg
     2341 gcgtccgtca cggcggcgtc aggggtggcg tcgggaagcg gggcgtcagg cgtggcatca
     2401 ggcgccgcgt caggcgcttc gacatcgacc tgttccggca tggtcgcggc gggcgtgacc
     2461 gtccagccct ggcctttccc atcgtaaaca aggtcgtaca tgcttccggc cggatcatcg
     2521 gggaaagctg caagaatcgc cgcgggtgaa tcctgggcag gcatgacggc cgccgcgacg
     2581 ttctggggac tgaaatccgg acacgtgcct gacgatacgc acgagcaacg gttctgagcc
     2641 ctgcacaggc cggctgtcgt atccagaacc agcatgtccg ggtcttccgg ttcgccgccg
     2701 acccaggaaa tggtccagcc ggaaagcggt gccccggtac tctttccatc gcgaccaatc
     2761 ccgatcccga cgattcgacc gacccagagg caatcgtacc caagggccgc ggtcgcaacg
     2821 gactcaagcg cctcgtgcgc gccaagggcc gtcacgccgg ttacataagg ggtgcgcgga
     2881 cagccgatca cgaaccggaa atcgccctgc ggtgaacacg cgacaggttc ggaaacgccc
     2941 tcggccggga gatcgaccgg aatatcgccc aggctggaac tgcacgcgac caggccgcag
     3001 gccatcacaa gcgccgggat gaaaacagga cgaatatcga actgaaggaa tcttccaggc
     3061 ttcatcgggt tctccggaaa tgccgcacgc gatgttagca gattgcggcc gatttcacag
     3121 cggacgaaga agaatccttg ccggggatcc gtcgccaccg tcaatcctca acggaaggcc
     3181 gagcatctcg tatcttcccc acggtacatc gccaaggaca agcccctcga tgattgcgac
     3241 accggccggc aacagcgcgc ggtgaacaag atccccttcc cttccgccgg gcccgatcga
     3301 aagatagtct atgccgacgg tgcggacgcc gcattttgca aggtaatcgg cgccatcgcg
     3361 cgtcaggtgg cagtagtcgg gccggaatgg ctgccgccac cattcgccga cgctgttggc
     3421 ggtcttcaac agcaggcgtt caccacgctc gggacagtgc tgacgcagga catcggcatt
     3481 gatttcgacc gcgtcccgaa ggccgattac cctggccggt ccgcagaggt gg
//
LOCUS       DAOPPA010000351         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28802_length_3528_cov_3.816585, whole genome shotgun sequence.
ACCESSION   DAOPPA010000351 DAOPPA010000000
VERSION     DAOPPA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28802_length_3528_cov_3.816585"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PKH54_10375"
     CDS             <1..1199
                     /locus_tag="PKH54_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD00342.1"
                     /translation="VRTLVASDRDDRNGCIFKVLEPAFQPSATDGFAEVVALGRLTGG
                     IAHDFANLAQVILGYTEILKQEAVDAGPASQPAILEDIAQIEQAALHAKDMVQQLGSF
                     CSRDIQAVMPCDPDRIIQDFLSLAKRVLGESIGLRFHPSQKPLCALADPAALKRAILK
                     LLLKVRDSGADDEVVAISTREVFIEADWPVGETGLEPGAWVKVSVSCQTTGWAVSAIQ
                     GSLDDGLAFIMGAMGGCLNVHESPDLGTTVSLYFKTGEEFDEHPVRPGKPQTTSPTRT
                     AVSATILVVEDDAPVRKLTAKALRQAGHSVIEAADGVEAVDAFMKQPDAIDLLVTDVI
                     MPGMNGREVCERINRVRPDLPVVFCSGYSSDLLQSEYMLNIHGLVIQKPFRSADLLAT
                     VARLLG"
     gene            1270..>3528
                     /locus_tag="PKH54_10380"
     CDS             1270..>3528
                     /locus_tag="PKH54_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOD00343.1"
                     /translation="MKKTMIPAMTLMLMTTCCAGSVATRPDGADAVLAAAVPPMPRGG
                     LWDAPVPPVWKEIDKLVEEQKFQAALDRIAELERNAAQSGDAVEQVRILVRKVSYETA
                     WNRWQDALNHLLASESSWPTDIVSQTTLGLYLASTIQGYRAFYGWEIARRPERSDPDL
                     TDVKTWSTRQLNDRAVATLFRLWAWRDTLKGIPASIASEFLEIDTFPEGIRGTMADAV
                     AYGLASALADSATWPVRFSGELWKLKPENLTADAARSSLTTIPEDRHPLLVMSDILRD
                     LEAMKAEAGNRPAALEARLELIRLVDGHSGAGKGWHRALTTHLETRLKSENGHDWWAE
                     GMALLAEMTMGLDDRDSFVKALEITRLCRDGRPESNGAARCEDVAGRILARELSDFGA
                     RAVDGAGSKAFVLESRNVDHAWFRSWKIDYAALVATPGIRDPESEIVARYRERENLPK
                     PDMTWDSAIDDPKDYKSHKSRISTPISDTGIWVVQISADPGFRKENNVIRTVIVAVGG
                     PIVNVEGFDDENNAIFRVYSGESGDPVPDAVIRIFRMNWSRGKAAKTLLAKGVTDANG
                     ESRFSRPTGGDGRFAAYVDAFGLTWPVSLPYYFDDMYRGSPYDHVQGVIMTDRAIYRP
                     GQQLSFKLMAYRDADEGSLPFVPDKSRTMTVELKDTNRETVASTTVTTNKWGTASGTF
                     EIPGGRMLGNWTIQADQLIRNTVRVEEYRIPSFSVDIETPGGLRLNRQAEVKGRADYF
                     FGLPVTGARVAWR"
BASE COUNT          730 a         1035 c         1126 g          637 t
ORIGIN      
        1 cggtgcgaac actcgtcgca agcgaccggg acgaccgcaa cggatgtatt ttcaaggtgc
       61 tcgaacctgc gttccagcct tccgccaccg atggtttcgc ggaagtggtg gcgctcggaa
      121 ggctgaccgg tgggatcgcc cacgacttcg cgaacctggc ccaggtcatt ctcggataca
      181 cggaaatcct gaagcaggag gcggttgatg ccggccccgc gtcccagcct gccatactcg
      241 aggacatcgc ccagattgaa caggcggcgc ttcatgcaaa agacatggtt cagcagcttg
      301 gaagcttctg cagccgtgac atccaggcgg tcatgccctg cgatccggac aggataatcc
      361 aggacttcct gagccttgcc aaacgggttc tcggggaatc gatcggactg cgtttccatc
      421 catcccagaa gcccctgtgc gcccttgccg atccggccgc cctgaagcgg gcgatcctga
      481 aactgctgct gaaggtcagg gactccgggg ccgatgacga agtcgtcgcg atttcaaccc
      541 gtgaggtgtt catcgaggct gactggccag tcggcgagac cggactcgag cccggggcct
      601 gggttaaggt gtccgtgtca tgtcagacga ccggctgggc cgtatccgct atccagggtt
      661 ccctcgatga tggactggcg ttcatcatgg gggcaatggg cggatgcctg aacgttcatg
      721 aatcgcccga ccttggaaca accgtttcac tgtacttcaa gaccggcgag gagttcgacg
      781 agcacccggt tcgtccaggc aagccgcaga cgacaagccc gacgaggacg gcggtcagcg
      841 caaccatcct ggtggtcgag gacgacgcgc ccgtccggaa gttgaccgcc aaggcgctgc
      901 ggcaggctgg tcattccgtg atcgaggccg ccgacggggt ggaggcggtc gatgccttca
      961 tgaagcagcc ggatgccatc gaccttcttg tgacggacgt aatcatgccc ggaatgaacg
     1021 gacgtgaagt ctgcgagagg atcaaccgcg tccgaccgga tcttccggtc gtcttctgca
     1081 gcgggtacag ttcggatctg ctgcaaagcg aatatatgtt aaatatacac ggtcttgtga
     1141 ttcagaaacc tttcaggtcc gccgatctcc tggcgaccgt tgccaggctg cttggctgat
     1201 gcctgtcggt tgccgggtcg catgatcgga atccatcgat cgcccgaatc gcataactgc
     1261 ggggaattta tgaaaaagac aatgattccg gccatgacgc tgatgttgat gacgacctgt
     1321 tgcgcgggaa gcgtggcaac ccgtccggat ggcgccgacg ccgtccttgc agccgcagtc
     1381 ccgccaatgc cccggggcgg gctgtgggac gcgccggttc cgccggtgtg gaaggaaatc
     1441 gacaagctgg tcgaggagca gaagtttcag gccgcgcttg atcgcattgc cgagcttgaa
     1501 cggaatgccg cacaaagcgg cgacgccgtt gagcaggtca ggatcctggt gcggaaggtt
     1561 tcctatgaaa cggcatggaa ccggtggcag gacgccctga accatctcct ggcttccgag
     1621 tcgtcatggc cgaccgacat cgtttcccag accacgctcg gcctttacct cgcttcgaca
     1681 atacagggct accgtgcctt ttacggctgg gaaatcgcgc gtcggccgga acgcagcgac
     1741 cccgatctga ccgacgtgaa gacatggtcg acgcgccagt tgaacgatcg ggcggtggcc
     1801 accctgttca ggctgtgggc ctggcgcgac actctgaagg ggattccggc atccatcgcg
     1861 tccgaattcc ttgagatcga cacgtttccc gaagggatcc gcggaaccat ggccgatgcc
     1921 gtcgcgtacg gccttgcttc ggccctggcc gactccgcga cgtggccggt gcggttcagc
     1981 ggcgaattat ggaagctgaa gcctgaaaac cttacggcgg atgcggcacg aagctcgttg
     2041 accacgatcc cggaagacag gcacccgcta cttgtcatgt ccgacatcct gagggacctc
     2101 gaggcgatga aggctgaggc cggaaacagg ccggccgcgc tggaggcaag gcttgagctg
     2161 atccggcttg ttgacgggca ctcaggcgcc ggcaaaggct ggcaccgcgc cctgacgaca
     2221 catcttgaga ccaggctgaa gtcggaaaac ggacacgact ggtgggcaga gggcatggcc
     2281 ctgctggccg aaatgaccat gggcctggac gacagggaca gcttcgtcaa ggctcttgaa
     2341 atcacacgcc tttgccggga cggaagacct gaatcgaacg gggcggctcg ctgcgaggac
     2401 gttgccggca gaatcctggc gcgcgaattg tccgacttcg gggcaagggc tgttgacggc
     2461 gctggcagca aggcgttcgt gcttgaatcc cgaaacgtag atcacgcctg gttccgctcc
     2521 tggaagatcg actacgcggc acttgtcgcc acccccggca ttcgcgaccc cgaaagcgaa
     2581 atcgtcgcca ggtaccggga acgcgagaac ctgccgaagc cggacatgac gtgggacagc
     2641 gcgatcgacg acccgaagga ctacaaaagc cacaaatcca ggatttctac gccgatttcg
     2701 gatactggaa tctgggtcgt gcagatctcc gcggatccgg ggttccgcaa ggaaaacaac
     2761 gtgatccgga ctgttatcgt ggcggttggc ggccccatcg ttaacgtcga gggcttcgac
     2821 gacgaaaaca acgcgatctt cagggtttat tcgggcgaat cgggcgatcc ggtgccggac
     2881 gcggtcatca ggatattcag gatgaactgg agccgcggca aggcggccaa gacattgctt
     2941 gcaaaggggg taaccgatgc caacggcgaa tcccggttct ccaggccgac tggtggcgat
     3001 ggtcgtttcg cggcctatgt cgatgccttc ggactgacat ggcccgtaag cctgccctac
     3061 tacttcgacg acatgtaccg cggatccccg tacgaccacg tccagggcgt tatcatgacg
     3121 gacagggcca tatacaggcc cggacagcaa ctttcattca agctgatggc ctatcgcgac
     3181 gcggacgaag gctcactgcc attcgtgccg gacaaatcgc gaacgatgac cgtcgaactg
     3241 aaggacacca acagggaaac ggtggcatcg acaaccgtca ccacaaacaa atggggcacc
     3301 gcgtctggaa cgttcgagat tccgggcggc aggatgcttg gaaactggac aatccaggcg
     3361 gatcagctca tccggaacac ggtgcgggtc gaggaatacc ggattccatc tttttcggtg
     3421 gatatcgaaa cgccgggcgg gctgcgcctg aaccggcagg cggaagtcaa gggaagggcc
     3481 gactacttct tcgggcttcc ggtgaccggc gccagggtcg cgtggcgg
//
LOCUS       DAOPPA010000352         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28976_length_3514_cov_3.317722, whole genome shotgun sequence.
ACCESSION   DAOPPA010000352 DAOPPA010000000
VERSION     DAOPPA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28976_length_3514_cov_3.317722"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1527)
                     /locus_tag="PKH54_10385"
     CDS             complement(103..1527)
                     /locus_tag="PKH54_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00344.1"
                     /translation="MTMPYCAPTTRRGLGRPSTSADLEARVWMAVDLVERSEAVADAL
                     YRFLPKCTFAVRDDVPTARIRGGDVPQIEFGTRFLDEELSDDRDFLFVLMHEIYHHVL
                     GHLRHSRGDKASRTFSNLANIAADMLVNRAVCERYFPEGVPLLNRMYDNHSLPSLLLL
                     PPPGRPGTPVSGRLRDRVLSNIAVALKGIGANPKLAHQVWTVYRMAWFGNAPFETVLE
                     RLLALFAKIDSMQFMTILLLGDHEGIEMPGELHKIAGDDEDGRGYGDGGDLGDEEIEV
                     DKAASDRDLAAALRRALEVQTGRYSINEPQRIPGVVCVPGRRDAAFLAGGAFPVIYQA
                     GTPVFDPLKLAHVYLDVSGSFDDYLPRIMGVLNGLRDQIADPVHQFSTVVSDISMARL
                     SQGAKCSTRGTNFNCVLTDAITRRYRQIIVITDGCGPVCEQVSTAFRTAMISLHLVLV
                     DSSPWDSRDSPLIALATSVLKMKE"
     gene            complement(1551..2630)
                     /locus_tag="PKH54_10390"
     CDS             complement(1551..2630)
                     /locus_tag="PKH54_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00345.1"
                     /translation="MIVKALVKSFSISGVTGYVYKDVNIPVQTMLNSDLQPFERVELG
                     FDALAMPLGNRSMLMDMNPLSLDVMPEADRGLPGLVVAATQPEALEMIVDHWIVGNSG
                     RCDAAVIQAREARAKGIPFLSSMVGLLAVDNRSRRMSLDEVESELGLVWSNDKFFQRF
                     SAVGGTRQEALLPEASGWRTNGTMGVVMEHDCRIGNVTMRRFAAMVPFGRERTRKVMV
                     SFGGEWQCESVRRIDPDVLNMFNKKGRAVYIGEGDLTGWQAWKAVMLVKVPERRGGCG
                     SDQTPDCLDGIPVGRLADARMPIPVADDVRVVESPAAARDILCRASRGFIISGLSGDF
                     IPQPVAKLRGRWLALCVPSHWAVAV"
     gene            complement(2627..>3514)
                     /locus_tag="PKH54_10395"
     CDS             complement(2627..>3514)
                     /locus_tag="PKH54_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00346.1"
                     /translation="NATAWLPASRALNVAGFVAATALSFYVAHAPLGQPINGEPAAGQ
                     YQVHGARISEYLNALSVYMASKEWPLDGRRLGMMHRGLVALLAARRVIRGQDVAPGEL
                     GSTIRDMLGFLLPFAATGRELPRLVLDGGHRFAMAALRGKPRSVAPSDFCDAVISMMN
                     GAEAGDRDAMSQMVTKILQAIDSPRDAEITIKAGAALFMLLSSQAVIERLPLESRQRV
                     IIEWADMMNIQTDNIDDFLQDSRRMEVPASIHPDRVDGFLRLSFSITHAICASSLPNI
                     EFESVVAKMGETIVNGGAE"
BASE COUNT          717 a         1104 c         1001 g          692 t
ORIGIN      
        1 gactatcgcg cttcacgact cgctcaaaca tgattcttag gcggggaact ccggtttatt
       61 ccgggctgat gataaaccaa atgcgtatgc atatggtcac tttcactcct tcatcttcag
      121 gacggaagtc gccagcgcta tcaatggact gtcccttgaa tcccagggcg aggaatcaac
      181 aagcaccaga tgaaggctga tcatggctgt cctgaaagcc gtggatacct gttcgcacac
      241 ggggccgcaa ccatcggtaa taacgatgat ctgccggtat ctccgggtga tcgcgtcagt
      301 caggacgcag ttgaaattgg ttcccctggt agagcatttc gccccctgtg acagccttgc
      361 catcgatatg tcgctgacca ccgtggagaa ctggtgaacc ggatcggcta tctggtcacg
      421 caacccgttc aggacaccca ttattcgggg aagataatcg tcgaatgagc ccgacacgtc
      481 cagatacaca tgggccagct tcaacgggtc gaaaaccggg gttcccgcct gatagattac
      541 cgggaacgct cctccggcaa ggaacgcggc gtcgcgacgg ccgggcacgc acaccactcc
      601 cggtattcgc tgtggttcat tgatgctgta acgacctgtc tggacttcca gcgcacgccg
      661 cagcgccgcc gcaagatccc ggtcgctggc agccttgtcg acctcgatct cctcgtcccc
      721 caggtcgccg ccgtcaccat acccgcggcc gtcttcgtcg tcgcccgcga ttttgtggag
      781 ttcgccaggc atctcgatgc cttcgtgatc gcccagaagc aggatcgtca tgaactgcat
      841 cgaatcgatc ttcgcgaaaa gagccaggag cctttccagc acggtttcga aaggcgcgtt
      901 gccgaaccag gccatgcggt aaacggtcca gacttgatgg gccagcttcg gattcgcccc
      961 aatcccttta agtgcgacgg cgatgttgct taaaaccctg tctcgaagcc tgcccgacac
     1021 gggagtgcca ggtctgccgg gtggcggcag cagcaacagt gagggcaggc tatggttatc
     1081 gtacattctg tttaaaagcg gcacgccttc cggaaaatac cgctcgcaga ccgccctgtt
     1141 cacaagcatg tcggcggcga tgttcgccag gtttgaaaac gtgcgcgacg ccttgtcacc
     1201 gcggctgtgc cgcaggtgcc ccagaacgtg gtggtagatt tcgtgcatca acacgaacag
     1261 gaagtcccgg tcgtcgctga gttcttcatc caggaagcgt gtcccgaact cgatctgcgg
     1321 tacgtcgccc ccacggatcc gtgcggtcgg tacgtcatcg cgcaccgcga aagtgcattt
     1381 gggcaggaac cggtaaagcg cgtcggcaac ggcttcggag cgttcgacaa gatcgaccgc
     1441 catccagacc ctggcttcca ggtcggccga ggtcgatggt cttcccagtc cccgtcgtgt
     1501 cgttggcgcg caatatggca tcgtcatcaa cccctccaaa atgaatcatt tcaaacggcc
     1561 acggcccagt gcgaagggac gcaaagcgcg agccagcgcc cgcgaagttt tgcgacgggc
     1621 tggggaatga agtcgccgct gagacccgag atgatgaatc cgcgcgaggc acggcacagg
     1681 atatcccgcg ccgcggcagg actttcgacc acgcgcacat cgtcggcgac cggaatgggc
     1741 atccgggcgt ccgccagcct gccgaccggt atcccgtcaa ggcagtccgg tgtctggtca
     1801 ctaccgcacc cgccccgtct ctccggaacc ttcaccagca tgaccgcctt ccaggcctgc
     1861 cacccggtca ggtcgccttc gcctatatac acagcgcgcc cctttttgtt gaacatgttc
     1921 aggacgtccg gatcgatcct gcgcaccgac tcgcactgcc attcaccacc gaacgaaacc
     1981 atcaccttgc gtgtccgctc cctgccgaag ggaaccattg cggcgaaccg gcgcatggtg
     2041 acattgccaa tcctgcaatc atgttccatg acgacaccca tggtgccgtt ggttcgccac
     2101 ccgctggctt cagggagcag ggcctcttgt cgcgttccgc caacagccga gaaccgctgg
     2161 aaaaacttgt cgttgctcca gaccaggccg agctcggatt cgacctcgtc aagactcatt
     2221 ctgcgggaac gattgtccac ggccaggagc ccgaccatcg aggacaggaa cgggattcct
     2281 ttcgcgcgtg cctcgcgagc ctggatcacg gccgcgtcgc atcgcccgct gttcccgaca
     2341 atccagtgat cgacgatcat ctcgagcgcc tccggttggg tggccgcgac caccagtcct
     2401 ggaagcccgc gatccgcttc aggcatcacg tctaacgaca gcggattcat gtccatcaac
     2461 atcgaacggt tgccaagcgg catggccagg gcgtcgaaac cgagttccac tcgctcgaat
     2521 ggctgcaggt cggagttcag catcgtctgg acagggatgt tcacatcctt gtacacgtat
     2581 cccgtaacgc ctgaaattga aaaggacttt acaagagctt tgactatcat tccgcacctc
     2641 cattaacgat ggtttcgccc attttggcga ctacggattc gaactcgatg ttgggcaaac
     2701 tgcttgcgca gatcgcgtgt gtgatgctga acgaaagccg caggaacccg tcgacgcgat
     2761 cggggtgaat gctggcgggc acttccatgc ggcgcgagtc ctgcaggaaa tcatcgatat
     2821 tgtcggtttg gatattcatc atgtcggccc attcgatgat cacgcgttga cgtgattcca
     2881 gaggcagcct ttcaatcacc gcctggcttg aaagcagcat gaagagcgcc gcgccggcct
     2941 tgatagtgat ttcggcgtcg cgtggggaat cgatcgcctg cagaattttg gtgaccatct
     3001 gcgacattgc gtcacggtca ccggcttcgg cgccgttcat catggaaatc acggcgtcgc
     3061 agaaatccga cggggccacc gaacggggct tcccacgcag cgccgccatc gcgaaacgat
     3121 ggccaccatc aagcaccagt ctgggaagtt cgcgcccggt ggccgcaaag ggcagcagga
     3181 atcccagcat gtcacggatg gtcgagccca gttcgccggg tgcgacatcc tgtccgcgga
     3241 tcacccgtcg ggccgccagc agcgcgacca gtcctcgatg catcatcccg aggcggcgtc
     3301 cgtccagggg ccattctttc gatgccatat agaccgaaag cgcgttcagg tactcggaaa
     3361 ttcgcgcgcc gtgcacctgg tattgaccgg ctgcaggctc gccgtttatc ggctggccga
     3421 gcggcgcatg cgcaacgtaa aacgataacg cggtagccgc cacaaagcca gcaacgttca
     3481 atgcgcggct tgccggtaac caggctgttg cgtt
//
LOCUS       DAOPPA010000353         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_28985_length_3513_cov_3.456912, whole genome shotgun sequence.
ACCESSION   DAOPPA010000353 DAOPPA010000000
VERSION     DAOPPA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28985_length_3513_cov_3.456912"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PKH54_10400"
     CDS             <1..1233
                     /locus_tag="PKH54_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOD00347.1"
                     /translation="GFEAGWARVDMTPAEPVPLAGYTHLSDRMSKGVHDPVFVRAVVM
                     KQEGGPAVALVMYDLVIVSEDLYQGLRARLSDTGLTVMCAATHTHSSFGGFWNAMLGR
                     AFLGRFRPGVLESLIELGEKAVRDALAGIGPAIVKTGTRKIPGLNGNRRDHNGPLDEE
                     LHVVTLEKQDSRALLFSYPGHPVISAERDHNVVSADFPGLAIRKIEGQFDFAMYMPGA
                     LGGVDVRFPREPVPIDDNLEMMAAPIAHTAMQVARDGNAFADERLVLAHSIRELHFDR
                     PRIRWSYDDDRKPFDFLLDALANGLARPVPRVARIEGFRIADVAVVGFPCDLGIGLSL
                     RVKQIGRELGLKGVVTMSESGGCVGYVHLPEDYSTTPADEGDAIQMGRYENAMNFFGH
                     QTGTRFLEAADGVLKELA"
     gene            1226..2410
                     /locus_tag="PKH54_10405"
     CDS             1226..2410
                     /locus_tag="PKH54_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00348.1"
                     /translation="MRKVTEKDAAAKVVSAMSELLDAVARGQGYSNLDPSILDGVTIP
                     VGHGLGGAEQAGKSAADLLNLASGIVSSAAAGTDNWKSGSVCCMQCGGSDCVHAMPPS
                     PTDVFAGYLATGKPNWVDFAHLCIDRHVEGFETLFVQRPGVVGVSIPGSDIESELLDS
                     FRDAAYRVVWAVNVGLVPADFEPVRSGQDRIALSLLAVLLVRGGRPAVRLNAVGLDTD
                     ALDHAAGNQGSRGPAERVRRILAATAGRLEAISSGLRHEARVTGPEDVNRLVDRLAGG
                     VGPVMNRLRSDLVAVFQGVDDRTRHGEQRARSGSRPTSAAFGDAESAPDESLLMDMVE
                     RTVIVAGPRGRCHVFTTDGRLVTSITLNDGDFQARIVHGRWRPLKESDISQFRQAISR
                     RR"
     gene            complement(2425..3156)
                     /locus_tag="PKH54_10410"
     CDS             complement(2425..3156)
                     /locus_tag="PKH54_10410"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008988007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD00349.1"
                     /translation="MNRIRLGVNVDHIATLRQSRGTKYPDPVLAAGIAELAGADQITI
                     HLREDRRHIQERDVRIMRETIGTVMNLELAANRDVLEIACLVKPDLATLVPEKRAELT
                     TEGGLAVVGREREVAATVEMLSRAGITVSLFINPDPAEVRASFECGARQIEIHTGFYA
                     DSRGERQRQELERIRVAAAEGARLGLQVAAGHGLDYVNTREVCHIPEIIELNIGHSIM
                     SRSFFVGLDQAVREMIDILREEEVG"
     gene            complement(3248..>3513)
                     /locus_tag="PKH54_10415"
     CDS             complement(3248..>3513)
                     /locus_tag="PKH54_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD00350.1"
                     /translation="LAGGYEKYPQVNYSRAVVDRPPIESLAQTNQAVREVEAALGEQG
                     RVVVRYSGTSKKIRVMVEGPDEGMVQSYAEQILAACGRDEILE"
BASE COUNT          632 a         1020 c         1129 g          732 t
ORIGIN      
        1 ggatttgaag cagggtgggc cagggtggat atgacaccgg ccgaaccggt tccgctggcc
       61 ggatacactc atctttcgga tcggatgtcg aagggcgtcc atgacccggt gttcgtgcgc
      121 gcggttgtca tgaagcagga aggtggcccc gcggtcgccc tggtcatgta cgatttggtt
      181 atcgtttccg aagaccttta tcaggggctg cgcgccaggt tgtcggacac cggactgacg
      241 gtcatgtgcg ccgcgaccca cacacattct tccttcggcg gattctggaa tgcgatgctc
      301 ggtcgcgcat tcctgggacg ctttcgaccc ggggtcctgg aatccttgat tgagctgggc
      361 gagaaggcgg tgcgggacgc cctggcaggg atcggcccgg ccatcgtgaa gacggggaca
      421 aggaagattc caggtctgaa cggcaaccgt cgcgaccaca atggcccgct tgacgaagaa
      481 ctgcatgtcg tgacccttga aaaacaggat tcaagggcct tgctcttttc atatccagga
      541 catccggtga tttccgccga acgggatcac aatgtggttt cggccgattt tccgggcctt
      601 gcgatcagga agattgaagg ccagttcgat tttgcgatgt acatgcccgg ggcgcttggc
      661 ggagtcgatg tcaggtttcc ccgggagccc gtcccgatcg acgacaatct ggaaatgatg
      721 gcggccccga tcgcccatac ggccatgcag gtcgcgcgcg acggcaacgc tttcgctgat
      781 gaacgtcttg ttctggccca ttcgattcgc gagcttcatt ttgatcgccc aaggattcga
      841 tggtcctatg acgacgacag gaagcctttt gatttcctgc ttgatgccct tgccaacggg
      901 ctggccaggc cggtgcccag ggttgcccgg atcgagggct tcaggattgc cgatgtggcc
      961 gtggtgggtt tcccatgtga ccttggcatc ggcctttccc tgcgcgtgaa gcagattgga
     1021 cgtgaactgg gcctgaaagg ggtcgtaacg atgtccgagt ccggtggatg cgtcgggtat
     1081 gttcacctgc ccgaggatta cagcacgacg ccggccgacg aaggcgacgc gatccagatg
     1141 ggacgttacg agaacgccat gaactttttc ggccaccaga ccggcacaag gttcctggag
     1201 gccgccgacg gggtcctgaa ggaacttgcg taaagtcacc gagaaggatg cggccgcaaa
     1261 ggtcgtttcg gcgatgagcg aactgcttga cgcggttgca cgcggccagg gttattccaa
     1321 cctggatcca tcgatcctcg acggcgtcac gattccggtc ggacatggtc tcggcggcgc
     1381 ggaacaggcg ggaaaatcgg ccgccgatct gttgaatctt gcatcgggaa tcgtgagttc
     1441 cgccgcggcc ggcacggaca actggaaatc cggttcggtc tgctgcatgc agtgcggcgg
     1501 ttcggattgc gtccatgcga tgccgccttc gcccaccgat gtctttgccg gttacctggc
     1561 gaccggcaag ccgaactggg tcgatttcgc ccacctgtgc atcgatcgtc atgtcgaggg
     1621 attcgagacg cttttcgtgc agcggcccgg cgtggtcggg gtgtcgattc cgggctcgga
     1681 catcgaatcg gaactccttg attcgtttcg ggatgccgcg tacagggttg tctgggccgt
     1741 caatgtcggt ctggttcccg ccgattttga acctgtgcga tccggccagg acaggattgc
     1801 actgtccctg ctggccgtgc ttcttgtcag gggcggacgg cccgctgtca ggctgaacgc
     1861 ggtcggtctt gacacggacg cccttgatca tgccgcggga aaccagggtt caagggggcc
     1921 ggccgagcgg gtgcgccgga ttcttgccgc gacggccggg aggttggagg caatttcttc
     1981 agggctgcgc catgaagcca gggtgaccgg tccggaagat gtcaatcggc tggtggacag
     2041 acttgccggg ggcgttggcc cggtaatgaa ccggcttcga tcggatcttg tggccgtttt
     2101 ccagggtgtc gatgaccgca caaggcatgg cgaacagcgt gcaagatcgg gaagcaggcc
     2161 gacatcggcg gcattcggcg atgccgagtc ggcccccgac gaaagcctgt tgatggatat
     2221 ggtcgaaaga acggtcatag tggccggccc gcgcggccgg tgccacgtct ttacgaccga
     2281 cgggcgcctg gtcacatcga tcaccttgaa cgacggcgat ttccaggcgc gaatcgttca
     2341 cggtcgctgg cgtccgttga aggaatcgga tatctcgcag ttcaggcagg caatttcaag
     2401 gcggcgctga ccggaagccg gaacttatcc gacctcctcc tcgcgcagga tgtcgatcat
     2461 ttcccgcacg gcctgatcaa gtccgacgaa gaacgaacgg gacattatgg agtgtccgat
     2521 gttcagttcg atgatttccg ggatgtggca gacttccctg gtgttgacgt agtcaagtcc
     2581 gtggccggcg gcgacctgca gtccaaggcg cgcgccctcc gcggcggcca cccgaatcct
     2641 ttcaagttcc tggcgctgcc tttccccgcg ggaatccgcg taaaatccgg tatggatttc
     2701 aatctggcgg gcgccgcatt caaatgaggc ccgaacctcg gccgggtctg ggttgatgaa
     2761 caggctgacc gtgatgccgg cacgactgag catctcgacc gtcgccgcga cctcgcgttc
     2821 gcgtccgacc acggcaagtc cgccttcagt ggtcagttcg gcccgctttt cgggaaccag
     2881 ggttgccaga tccggcttga ccaggcacgc gatttcaagc acgtcccggt tggccgcgag
     2941 ttccaggttc attacggttc cgatcgtttc gcgcatgatg cgcacatccc gttcctggat
     3001 gtgccggcga tcctcgcgaa ggtggatggt aatctggtcg gcgcccgcga gttcggcgat
     3061 accggcggca agcaccggat cagggtactt cgtcccacgg gactggcgaa gggtcgcgat
     3121 gtgatcgacg ttgacaccaa gtctgatacg gttcatttca gtcaccacgg gggcagatgt
     3181 aatccgccca tgaaaaccgg gcgggaggat gtaacccgtc catacggaaa gcaggcgggc
     3241 cggcccttca ttccaggatt tcgtcccgtc cgcacgcggc aagaatctgc tcggcgtaac
     3301 tctggaccat gccctcgtcg ggaccttcta ccataacgcg gatcttcttt gaagtgcccg
     3361 agtaccttac aacgacgcgt ccctgctcgc ccagcgccgc ctcgacctcg cgaacagcct
     3421 gattcgtctg cgccaggctt tcaatcgggg gacgatcgac cacggccctg ctgtaattga
     3481 cctgaggata cttctcgtat ccgccggcaa gtt
//
LOCUS       DAOPPA010000354         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29161_length_3497_cov_3.625218, whole genome shotgun sequence.
ACCESSION   DAOPPA010000354 DAOPPA010000000
VERSION     DAOPPA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29161_length_3497_cov_3.625218"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PKH54_10420"
     CDS             complement(<1..129)
                     /locus_tag="PKH54_10420"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002079641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOD00351.1"
                     /translation="MKDTDCSELVTLRGRIDQVNRGILELLNERARLVLEVQAVKER"
     gene            complement(140..367)
                     /locus_tag="PKH54_10425"
     CDS             complement(140..367)
                     /locus_tag="PKH54_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00352.1"
                     /translation="MKALILVMSVLLTVACAACYPVVVAPGCQQQVNDCLRGCSASPV
                     GQPYEYSPTSPIDTRSDCERHCHESCAQQWK"
     gene            complement(380..1690)
                     /locus_tag="PKH54_10430"
     CDS             complement(380..1690)
                     /locus_tag="PKH54_10430"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOD00353.1"
                     /translation="MRTNGFVIAFAAAMALAVGCADSVEEVDIYATNFEPGVEMRDGV
                     RILRLKGTPYEMGKQHATFLHDELVKGVEFLETTELGLLEFIARDYGFLDDAMAQSYP
                     DVIDECEGMAEVAGAEGWTMDRCMALAYGEIVLDFFEAGMLQCSQFAAAGPATVDGEL
                     IHGRNLDWGDMSYLLDYPTIMVRAPEGLIPYVVVGFPGCVAAYNGMNTAGLSIATNEN
                     GSMNDLDREGVPTVQGVNYMLSHFTKIDEVIDYIVNSDRMSAENMVVTSGHEKRAVVF
                     QMTASHIHYEELGDDGVNYITNHFVTEDMQPYEDPKDPGTSTYCRYMRLRQLLEPGFE
                     NVRDDKVYGSVDPAVAAGILRDRYNPIDMIEYPKGQFDVETATGGAIAVNAAIYSIVF
                     KPASGQFWFAYGSKPIPDNPFVGFNVQELLGNDPAAVAVPAVVE"
     gene            complement(1920..2762)
                     /locus_tag="PKH54_10435"
     CDS             complement(1920..2762)
                     /locus_tag="PKH54_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD00354.1"
                     /translation="MKEISAPYAAPFIKWAGGKSQILPWIEKMLPRQIGNYFEPFIGG
                     GAVFFKLASQGTVARSFLCDANPHLVTTYRVVRDEPESLIKYLDELKLRNSDEYFYQA
                     RNRFNHEQMSDVERAALLIYLNKTCFNGLFRVNSKGHFNVPVGRYVNPGIYDAQQIQA
                     CSAALKHAEIECGDFESILTDVGPGDFVYFDPPYVPLTKTAHFTSYARDDFTFDDQVR
                     LSNVFKRLSEVGARVLLSNHATPELISLYDGFDISIVPARRMINRDASRRMAPIDEVL
                     IRNY"
     gene            complement(2764..3159)
                     /locus_tag="PKH54_10440"
     CDS             complement(2764..3159)
                     /locus_tag="PKH54_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00355.1"
                     /translation="MPGRSKAPENGKDLEKRVVEIAEGLGLVVETQVRVARRIWGAVR
                     QIDVVLTHPVSRLRLGVECKYQAVSGSAEEKIPAIVEDIDAWPIRGIIVISGEGFTAN
                     MKSSLFSTGKVVELEDLEAWLKLFFGLDI"
BASE COUNT          796 a         1066 c          897 g          738 t
ORIGIN      
        1 gcgttcctta accgcctgaa cttccaggac cagtctggcg cgctcgttaa gcagttccag
       61 gatgccacgg ttgacctggt caatccgccc gcgcagggtc accagttcac tgcaatcggt
      121 gtctttcatc ggcagccccc tatttccact gctgggcaca ggactcgtga caatgacgct
      181 cgcagtcaga cctggtgtcg atcgggctgg tcggactgta ttcatagggc tggcccacgg
      241 gcgacgccga acaaccgcgc aggcagtcgt tcacctgctg ctgacagccg ggggcgacga
      301 ccacgggata gcatgccgcg cacgcgaccg tcagaagcac cgacatgacc agaatcaatg
      361 ccttcatacc ctgccccgcc tactcgacga cagccggaac cgcaaccgcc gccgggtcgt
      421 tacccaggag ttcctgaacg ttgaaaccga caaacggatt gtccgggatc ggcttcgatc
      481 cataggcaaa ccagaactgg ccggacgcgg gcttgaacac tatcgaataa atcgccgcat
      541 tgaccgcgat cgccccgccg gtggcggttt cgacgtcgaa ctggcccttc gggtactcga
      601 tcatgtcgat cgggttgtac ctgtcgcgca ggatgccggc ggcgaccgca ggatcgaccg
      661 aaccatagac cttgtcgtca cggacgtttt cgaagcccgg ttccagaagc tgacgcaggc
      721 gcatgtaacg gcaataggtc gaggttcccg gatccttggg gtcctcgtac ggctgcatgt
      781 cctcggtgac gaaatgattg gtgatgtaat tcacgccgtc atcgccgagt tcttcgtaat
      841 ggatgtgcga cgcggtcatc tggaaaacga ccgccctctt ctcgtggccg gacgtgacga
      901 ccatgttctc ggccgacatg cgatccgaat tgacaatgta atcaatgact tcgtcgatct
      961 tcgtgaagtg cgaaagcatg tagttcacgc cctgcaccgt cgggacaccc tcgcgatcca
     1021 ggtcgttcat ggacccgttt tcattcgtgg cgatcgaaag accggccgta ttcatgccgt
     1081 tgtatgcggc aacgcaaccg gggaaaccca cgactacgta tggaatcagg ccttccggcg
     1141 cacgcaccat aatcgtcggg taatcgagaa ggtaggacat gtcgccccag tccaggttcc
     1201 ggccatggat cagttcacca tcgacggtgg ctggacccgc cgccgcgaac tgggaacact
     1261 gcaacatacc ggcctcgaag aaatcgagca cgatttcgcc gtacgccagg gccatgcagc
     1321 ggtccatcgt ccagccttcc gcgccggcca cttccgccat gccttcacat tcgtcgatca
     1381 cgtcgggata cgactgtgcc atcgcgtcat ccaggaagcc gtagtcccgg gcgatgaatt
     1441 ccagcagacc cagttcggtc gtttccagga attcgacgcc cttgaccagt tcatcatgca
     1501 ggaacgtcgc gtgctgcttt cccatttcgt aaggcgtgcc cttcaggcgc agtatcctta
     1561 cgccgtcgcg catctcgacg cccggttcga aattcgtcgc gtagatgtcg acttcttcaa
     1621 cggaatcggc acagccgact gccagagcca tggccgccgc gaaagcaatc acaaaaccgt
     1681 tcgtccgcat tgttccacca gaaaaaatcc gcccgccgat tctacaccaa atgcacgagc
     1741 gaattacacc cgcccgcggg gcccgcccca cgggcgcttc tcgtgctctc ggcgcgcgac
     1801 ggtcattaaa gccccgcagg gtgtcgcgcg cctccggggc gttccgctac gggtaccccg
     1861 cggccccttc ggtgttacgc ccaacgtttc agtcgtcagg ggcgtaagac cttgtttgct
     1921 cagtagttcc tgatcaggac ttcatcaatc ggcgccatac ggcgtgacgc atcgcgattg
     1981 atcatacggc gggctggaac gatggaaata tcaaacccat cataaagaga gataagttcc
     2041 ggggttgcat gatttgacag cagtaccctg gccccgactt ccgaaagcct cttgaagacg
     2101 ttcgaaagcc tgacctgatc gtcgaacgtg aagtcatcac gggcatagga cgtaaaatgg
     2161 gcggtcttcg tcaaaggaac gtacggggga tcaaaataga cgaaatcacc aggccccaca
     2221 tcagtcaaga tgctctcaaa atctccgcat tcaatctccg cgtgcttcaa agccgccgaa
     2281 caagcctgaa tctgctgcgc gtcataaatg cccggattaa cgtaacggcc aactggcacg
     2341 ttgaagtggc ctttggagtt caccctgaag agcccgttga aacacgtctt attcaaatag
     2401 atcagcaggg ctgccctttc cacgtccgac atctgctcgt ggttgaagcg atttcgagcc
     2461 tgatagaaat attcgtcact attacgaagc ttcaactcgt caaggtactt gatcaaggat
     2521 tccggctcat ccctgaccac gcgatacgtc gttacgaggt gcggattggc atcacatagg
     2581 aaagaccgcg ccacggtgcc ctgcgatgca agcttgaaga ataccgcgcc gccaccgatg
     2641 aaaggttcga aataattgcc gatttgccgt ggaagcatct tctcgatcca cggaagaatc
     2701 tgacttttcc cgccagccca tttgataaac ggggcggcat atggtgcaga gatttctttc
     2761 aaatcaaatg tccaaaccaa agaagagttt caaccaggct tccaaatctt caagttcaac
     2821 gaccttcccc gtcgaaaaca aggaggactt catgttggcc gtaaaacctt cgccggaaat
     2881 gacaattatt cccctgatgg gccaggcgtc gatgtcctca acaattgccg ggatcttctc
     2941 ctctgccgag cccgagaccg cttggtactt acattcgacg cccaggcgaa gtcttgacac
     3001 cggatgggtt aacaccacat cgatctgcct caccgcaccc caaattcgcc gggcaacgcg
     3061 gacctgagtt tcaaccacaa ggccaagccc ctctgcaatt tccacaaccc gtttttcaag
     3121 atcctttccg ttttctgggg ccttcgacct tcccggcatt ccaaattcac cacgatgaaa
     3181 acccgccccg attagaaacc gataattgtg ctgtggtcaa cagttccctt tgcgctatta
     3241 ccgtgaccag cccaacaatt ctttgcatga agacattgaa aaatacgtct gactacgatt
     3301 accattctct ttacagacat cgggtaaccc cttgatcttg cgcaagaatt atccggcgct
     3361 aatatcattc atctagttaa agtcggcaca tttctcaaaa ttttcaacag gttattgaag
     3421 tcgcgctccg acaatcgctg ggcgtacgcc gatggccagg gcaggcggcg gggaagcctg
     3481 gagcggcata ccccacc
//
LOCUS       DAOPPA010000355         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29181_length_3495_cov_4.100872, whole genome shotgun sequence.
ACCESSION   DAOPPA010000355 DAOPPA010000000
VERSION     DAOPPA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29181_length_3495_cov_4.100872"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKH54_10445"
     CDS             complement(<1..724)
                     /locus_tag="PKH54_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HOD00356.1"
                     /translation="MSGTGNEFISRLLLEHRLVDVESLAKAEEARGQRQERGLVETLL
                     DMGLMEEKRYLAAFGSILGLDVLESLPLDDVDLNLLKLVPITWARENSVLPIRRDDDR
                     IVAAISNPLNLSIVDDLKALFGARVEIVLASNDVVEDGINQAYQRLTGLEGTASAVFG
                     DDENDLEKAAADLEESSRDLLDVTDEAPVIRLVNSILGQAVKERVSDIHIEPFEKLLS
                     VRFRKDGILREVLRPPKRFQASI"
     gene            complement(782..>3495)
                     /gene="gspD"
                     /locus_tag="PKH54_10450"
     CDS             complement(782..>3495)
                     /gene="gspD"
                     /locus_tag="PKH54_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOD00357.1"
                     /translation="PGYRAAQRLGISGLAVVAALLLLSHPQEAAAQAGGGAQVLPTGT
                     GAVPPPPLPGAKADKGAKAAGDAAASAKGGPAATQAATPAQATAQKAPETGIPPEVDE
                     VVTFKTNFEKDVKCRPLPLNAKINVDFEEAPIKDVVKFIACITQRNFLIGTNLKSGKT
                     VSIMSTTPVTVYEAYKAFLASLDINGLTIVPSGPFYKIVETAKAKTEVVPVYSDNEKV
                     PAEDRLVTRIVNLKYIDVNDVSQVLTKFKTAAGDVAIYQPGNSLIITDLGSNIVRMLK
                     FINELDAPTGREKIWVRPVQYAAASEMATLITSIFGEKGGSSGSGQPRKGAPAQQATG
                     EDAAASRTPEGASIVSKVIPDERTNQLIIVATPAAYMKIDRLLRRMDMPIEGEGQIHI
                     YYLENANAEDVASTLSSLASGSGSGKKGGKGASAGGAASLFSGEVKITAHKPTNSLVI
                     ESTLKDYMALKRVIQQLDIRRKQVYVEAVIMEISSNKNKNFGISGSFGASLNIGGESV
                     PLLMGLGGLGLSGLDVNQLTSGGMAFGMQGPMVNLNAGSTGGTTGTLSIPAYGFAIQA
                     IQTNSDVNVLSTPHILTMDNEEAEIVVGKQIPYQASSGTGLYSLPGNYGGLTGATSGL
                     ATSALSSLTGSLGYGGYVQRIDVDMTLKITPQISESNFVRLQIQQQLDDVESMDTTLG
                     PTTSKRKVSNTVVVKDQQPVVIGGLIRDVETSGIDKVPLLGDIPVLGALFRRSVKTVE
                     KRNLLMIIIPYIIEDPSDLQRIHDQKLEEMREFAEYMATRKQEYQGGIDYRKKIGLIE
                     DITRTMEKVRKDREMLEQTTFDNVDLVGAPAGHDLEFDPLSAAPAAVQQESGQGSGDV
                     VPAKKQGASAGQSKAPVLPPRAEPSAAPAPAVEMGAEEQEPTVPLID"
BASE COUNT          632 a         1090 c         1031 g          742 t
ORIGIN      
        1 tgattgacgc ctggaaccgt ttgggcggcc taagcacctc gcgaagaatc ccgtccttcc
       61 ggaagcgaac cgaaagaagc ttctcgaaag gctcgatatg gatatcgctg accctctcct
      121 tgacggcctg tccgagaatt gaattgacca gcctgattac cggcgcctcg tcggtgacgt
      181 caagcagatc gcggctgctc tcctcaaggt cggccgcggc cttttccaga tcgttctcgt
      241 cgtcgccgaa aaccgccgag gccgtgcctt ccagccccgt caacctctgg taagcctggt
      301 tgattccgtc ctcaaccacg tcattggatg ccagaacaat ctcgacacgg gcgccgaaca
      361 gcgccttcag gtcatcaaca atcgacaggt tcagcggatt tgatatcgcg gcaacaatcc
      421 tgtcgtcgtc cctgcggatt ggaagcaccg agttttcacg ggcccacgtg atgggaacaa
      481 gcttgagcag gttcaggtca acatcgtcca gcggcaggga ctcgagcacg tcaaggccaa
      541 gaatcgatcc aaaggccgcc aggtatcgct tctcctccat caggcccatg tccaggaggg
      601 tctcgaccag gccacgctcc tggcgctggc cgcgagcctc ctcggccttc gccagggact
      661 cgacatcgac cagcctgtgc tccagaagca acctgctgat gaattcattt ccagttccgg
      721 acatctgact ctccgcaagc cccgccgaac gacgggacgc tcccgacacc attcaggcag
      781 gtcagtcaat cagggggact gtcggctcct gctcctcggc acccatctcc acggccggcg
      841 cgggcgcggc ggaaggctcg gcccgaggtg gaagaaccgg tgccttcgac tggcccgctg
      901 atgccccctg cttcttcgcc ggaacgacat cgccgctgcc ctggccggat tcctgctgga
      961 ccgcggcagg tgcggcggaa aggggatcga actcgagatc gtgtccggca ggcgcgccga
     1021 ccaggtcaac gttgtcaaac gtggtctgtt cgagcatctc gcgatccttc ctgacctttt
     1081 ccatcgtccg ggtgatgtcc tcgataagcc caatcttttt ccggtagtcg atgccaccct
     1141 ggtattcctg cttcctggtg gccatgtatt ccgcgaactc gcgcatttcc tcgagtttct
     1201 ggtcatggat tcgctgaaga tccgacggat cctcgataat gtagggaatg atgatcatca
     1261 gaaggttgcg cttctcgacc gtcttgacgg atcgccggaa gagcgcgcca agaaccggta
     1321 tgtccccgag aagcggaacc ttgtcgatgc cggacgtctc gacatcgcgg atcaggccgc
     1381 cgattacgac cggctgctga tccttgacga cgacagtatt ggacaccttt cgtttcgacg
     1441 tggttgggcc aagggtcgtg tccatcgact cgacatcgtc aagctgctgc tggatctgca
     1501 gccgcacgaa gttggattca ctgatctggg gcgttatctt cagcgtcatg tcaacgtcga
     1561 ttcgctggac gtatcccccg taaccaaggc tcccggtcag tgacgaaaga gcggatgttg
     1621 ccaggccgct ggtcgcgccg gtcagcccgc cgtagtttcc aggcaggctg tacaggcccg
     1681 tgccggaact ggcctggtaa ggaatctgct ttccgacgac tatctcggct tcctcgttat
     1741 ccatggtcag gatatgcgga gtcgaaagga cattgacgtc ggagttggtc tggattgcct
     1801 gaatcgcgaa accgtaggcg gggatcgaaa gcgttccggt cgtgccgccg gtgctgcccg
     1861 cattaaggtt gaccatcggc ccctgcatgc cgaaagccat gccgccgctg gtaagctggt
     1921 tgacgtcaag tccggaaagc ccaagcccgc ccagccccat cagcagcgga accgactcgc
     1981 cgccgatatt caggctggcc ccgaagctgc ccgatatgcc gaagttcttg ttcttgttgg
     2041 acgaaatctc cattatcacg gcctcgacat agacctgctt ccgcctgatg tcgagttgct
     2101 ggatgacgcg cttgagcgcc atgtaatcct tcagggtgct ttcgatgacc aggctgttcg
     2161 tcggcttgtg ggcggttatc ttgacctcgc cggaaaagag cgatgcggcc ccgcccgcgg
     2221 aggcgccctt gccgcccttc tttccggagc cggaaccgga agcaagcgat gacagcgttg
     2281 acgcgacgtc ctcggcattc gcgttctcaa ggtagtaaat gtgaatctgg ccctcgccct
     2341 cgatgggcat gtccatccgg cgaagcagcc tgtcgatctt catgtaggcg gccggcgtgg
     2401 ccacgatgat gagctggttg gtgcgctcgt cagggatgac ctttgaaacg atggaagcgc
     2461 cttcaggtgt cctggaagcg gcggcatcct caccggttgc ctgctgcgcg ggggcaccct
     2521 tgcggggctg ccctgaaccg gagctacccc ccttctcccc gaatatcgac gttatcagag
     2581 tggccatctc ggacgcggcc gcgtattgaa ccggccgaac ccagatcttc tcgcgtccgg
     2641 tcggggcgtc cagttcgttg atgaacttca gcatcctgac gatgttgctt ccaagatccg
     2701 tgatgatcag ggaatttccg ggctgataga tcgcgacgtc gccggccgcg gtcttgaact
     2761 tggtcaacac ctggctgacg tcgttgacgt cgatgtactt caggttgacg atcctcgtga
     2821 ccagcctgtc ctcggccggc accttctcat tgtccgaata gaccggaacg acctcggtct
     2881 tggccttggc cgtttccact atcttgtaaa agggacccga gggaacaatc gtcagcccgt
     2941 tgatgtcaag cgacgccagg aaagccttgt acgcctcata gaccgtgacg ggcgtggttg
     3001 acattatgga aacggtcttg ccgctcttca ggttggtgcc gatcaggaag ttcctctgcg
     3061 ttatgcaggc gatgaacttc acgacgtcct tgatcggggc ctcctcgaaa tcgacattga
     3121 tctttgcatt caggggcaga ggccggcact tcacgtcctt ttcaaaattg gtcttgaacg
     3181 tgaccacttc atcgacttcc ggcgggatcc ccgtttccgg ggccttctgg gcggtcgcct
     3241 gcgccggcgt cgcggcctgt gtcgcggccg ggccaccctt tgcggatgcc gcggcgtcgc
     3301 ccgccgcctt ggcacccttg tccgccttgg cacccggcag cggcggtggt ggaacagcgc
     3361 ccgtgccggt cggaaggacc tgcgcgccac cgccggcctg ggccgccgct tcctgcggat
     3421 gggaaagaag cagcagggcc gccacgacgg cgagtccaga tattccaagt ctttgagcag
     3481 cccggtaacc cggtt
//
LOCUS       DAOPPA010000356         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29459_length_3470_cov_3.856223, whole genome shotgun sequence.
ACCESSION   DAOPPA010000356 DAOPPA010000000
VERSION     DAOPPA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29459_length_3470_cov_3.856223"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKH54_10455"
     CDS             <1..315
                     /locus_tag="PKH54_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00358.1"
                     /translation="WRFSPSGSWPSWASGRDWGATHCQGLISGVVGSAAWQFVADGSL
                     SSIQLPDFSRAIGYAPLGPGPKRMNLTCSRTPRFDIGNFDYRALSTTIRETYSVDMAS
                     FY"
     gene            391..2718
                     /locus_tag="PKH54_10460"
     CDS             391..2718
                     /locus_tag="PKH54_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD00359.1"
                     /translation="MNRFGGILPFMVMAFVTLGTVTDAFSAPSRKAGRKPRITVESAV
                     ESLAVGLGAEYDEAQAFLQSGNDGLAARKGPVAAVLRKYPKDADVARRMLDMLSGFGT
                     DGCSFAASLVSAGLPAFVAEILDRYPAALRCNQLDKAVSSIAGWAPDPSQSREAELFV
                     LDVISAFKLSMNATGAMAACAWLSSGPASVRVAAADLIAAVKPSKGLDCLSLAFDSEK
                     NGENRELAGEYLEAIARFGGEASFPHLLNAFNNVNHFEKACAAVVRAGDTGIKTLVRA
                     VRSTSGRDPLLKECFSRHAAVSRPYVLALLGDRSRDMRRFALDFFTANHFPEALERFK
                     AGFRISDSTVVPDIPRHDLFEAIASYPVEEISDMVELALADNDDQIRDRALELIQKRK
                     SLAFAPALRTVAEFDPNRVSRIHALKIIMMLLDSTAEPLVRKLAEYEETTVATEAARV
                     LGFIGSQESIEVLRKLARTHKGTERGLAALQALAFLGITRDPMNAMYVGPVEPKAKKV
                     DGTVACGRFEASRVGSRGPLVIALPGGPGMDSAWAGGWIEELGRKATVARVEPVNRES
                     TTDAVLSAQELDCLVESMGQEKAVLVGEGLGGTSAMWLAFQAPARVAGLVTISAPLPG
                     AVNAMDAVLIRNLPQPFDAIARELVEQSAEFRPAAVNYYLLKAFSPALAGGMSKAARA
                     MHLRWDSERQAAARAELSRSEVRFNAMEVDRPALWILPAEMMLPEELESYRSLAAERP
                     DKYRVFAIDGDCGTSPGISCSGVLVKQLRRFIADL"
     gene            2766..>3470
                     /locus_tag="PKH54_10465"
     CDS             2766..>3470
                     /locus_tag="PKH54_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00360.1"
                     /translation="MTGDLNGAGQQVTEAALAAASSTAARISACVGSVFLGKPDVLRQ
                     SIIALVAGGHVLLEDVPGAGKTVLARTLAASIGGRFSRVQFTSDLLPSDITGVSVYSS
                     RDESFVFHQGPVFANVLLADEINRASPRTQSALLEAMSEREVTVDSKTRELPRPFFVI
                     ATQNPHEHSGTYPLPESQLDRFLMRLSIGYPDRESERRIAMVQGFSGNLGIESVVSPD
                     EVAAAQQVARIIPFPGF"
BASE COUNT          614 a         1028 c         1109 g          719 t
ORIGIN      
        1 tggcgattct ctccaagcgg tagctggccc tcttgggcgt ccggccgaga ctggggcgcc
       61 acacactgcc aggggctgat ttcgggtgtc gtcgggtcgg cggcctggca gttcgtggcc
      121 gacggaagcc tgtcatcgat ccagctcccc gatttttcca gggcgattgg ctacgcgccc
      181 ctgggaccgg gtccgaagag gatgaacctg acgtgttcgc ggacgccgag gttcgacatc
      241 ggcaatttcg actatcgcgc gctttccacc acgattcgtg aaacctattc ggtcgatatg
      301 gcatcttttt attgacgggc ctttccccga ctcatccaac cggcatctcc gtgctacaat
      361 cgttccggtt ctctttgcag cggggcgcgg atgaacagat ttggcggaat ccttcctttt
      421 atggtcatgg ctttcgttac gctggggacg gtaaccgacg cgttttccgc accttcccga
      481 aaggccgggc gaaagccgcg aatcacggtc gagtccgctg tcgagtcgct ggccgtgggg
      541 cttggtgccg aatacgacga ggcccaggcc ttccttcaat ccggcaacga tggccttgcc
      601 gccagaaagg ggccggtggc cgccgtcctg aggaagtacc cgaaggacgc cgatgttgcc
      661 cggcgcatgc tggacatgct ttcgggtttc ggaaccgatg gctgctcgtt cgccgcatcg
      721 ttggtgtctg ccgggttgcc ggcctttgtc gccgagatcc tggatcgcta cccggcggcc
      781 ctccgctgca atcagcttga caaggccgtc tcctccatcg cgggctgggc gccggatcct
      841 tcgcaatcgc gcgaggccga gcttttcgtg cttgatgtga tctccgcctt caagctttcg
      901 atgaacgcca ccggcgccat ggctgcatgt gcgtggctga gttccggccc ggcaagcgtc
      961 cgggtcgccg ccgcggatct tattgcggcc gtcaagccgt cgaagggcct ggattgtctc
     1021 tccctggcgt tcgactcgga aaagaacggt gagaacaggg agcttgccgg tgagtacctc
     1081 gaggcaatcg ccaggtttgg cggggaagct tcattcccgc acctgttgaa cgccttcaac
     1141 aacgtcaacc actttgaaaa ggcgtgcgcc gccgtggtaa gggcgggcga caccggcatc
     1201 aagaccctgg ttcgtgcggt cagaagcacg tcgggtcggg atcctcttct gaaggaatgc
     1261 ttctccaggc acgccgcggt ctccagacca tacgtccttg cgctgctcgg ggacagatcc
     1321 cgtgacatgc gccgcttcgc cctggacttc ttcacggcca accatttccc cgaggcactt
     1381 gaacgtttca aggccggttt caggatatcg gattcaaccg ttgttcccga tatcccgcgc
     1441 cacgatctgt tcgaggcgat tgcgtcgtat ccggtcgaag agatcagcga catggtcgaa
     1501 ctggccctgg cggacaatga cgaccagatc cgcgacaggg ccctggaact gatccagaag
     1561 cgcaagtcgc tggcgttcgc ccccgcgctg cgtacggttg cggaattcga tccaaacagg
     1621 gtcagcagga tccacgccct gaaaatcata atgatgctgc ttgattccac cgcggaaccc
     1681 ctggttcgga agctcgccga gtacgaggag accaccgttg ccaccgaagc ggcgcgtgtt
     1741 cttggcttca tcggttccca ggagtccatc gaggtgctgc gcaagcttgc caggacccac
     1801 aagggaaccg agagggggct ggctgccctg caggccctgg cattcctggg gataacccgc
     1861 gaccccatga acgcgatgta tgtcggcccc gttgaaccga aggcgaaaaa ggttgacggc
     1921 accgttgcct gcgggcgatt cgaggcttcg agggtcggtt caagggggcc ccttgtaatc
     1981 gcgcttcccg gcgggcctgg aatggattcc gcctgggccg ggggatggat cgaggaactt
     2041 ggcaggaagg caaccgttgc ccgggtcgag ccggtcaatc gggagagcac gaccgatgcc
     2101 gtgctgtcgg cccaggaact ggactgcctg gtggagtcca tgggccagga aaaggcggtg
     2161 cttgtcggtg aagggctggg gggaacctcg gcgatgtggc tggcatttca ggcaccggcc
     2221 cgtgtagccg gcctggtcac catatcggcg ccgttgccag gtgcggtgaa cgcgatggat
     2281 gcggtgctga ttcgcaacct gccgcagccg ttcgatgcga ttgcccgcga actggtcgaa
     2341 cagtcggcgg agttccgccc ggcagccgtc aattattacc tcttgaaggc gttttccccg
     2401 gccctggccg gcggcatgtc caaggcggcg cgtgcgatgc acctgcgctg ggattcggaa
     2461 aggcaggctg ccgccagggc ggaactgtcc cgttccgagg tacgcttcaa tgcgatggaa
     2521 gtcgatcgac cggcactgtg gattctgccg gccgaaatga tgcttccgga ggaattggag
     2581 tcttacaggt cgcttgcagc ggaacgtccc gacaaataca gggtttttgc gattgacggc
     2641 gattgcggga cgtccccggg gatatcctgt tccggtgtgc ttgttaaaca gcttcgcagg
     2701 ttcatcgcgg atctgtgaac ggttcgcgcg ccggcgaata gaaaacataa ggctggattg
     2761 tgaatatgac gggtgatttg aatggtgccg ggcagcaggt taccgaagct gcccttgccg
     2821 ccgcatcttc cacggcggcc aggatttcgg cctgtgtcgg cagcgtgttc ctgggaaaac
     2881 cggacgtttt gcggcagtcc ataatcgccc ttgtcgccgg tggccacgtg ctcctggaag
     2941 acgtgccggg cgccgggaag accgttcttg cacggacgct ggcggcatcc atcggcggtc
     3001 gcttttcaag ggtacagttc acgtccgacc ttctgccgtc cgacattacc ggcgtcagcg
     3061 tctattcgtc cagggatgaa agtttcgtct ttcaccaggg gccggtcttt gccaacgtgc
     3121 tgcttgccga cgaaataaac cgcgcaagtc caagaaccca gagcgcgctg ctggaagcaa
     3181 tgagcgaaag ggaagtcacc gtcgattcga aaaccaggga acttcccagg ccgtttttcg
     3241 tgatagcgac acagaatccc cacgagcatt ctggcactta cccgttgccg gagtcccagc
     3301 ttgatcgatt cctgatgagg ctctcgatcg gttatcccga tcgcgagtcg gagcggcgca
     3361 tcgcgatggt ccagggcttt tcgggaaatc ttggcatcga atcggtggtt tcgccggacg
     3421 aggtcgccgc cgcgcagcag gtggcgcgaa taatcccgtt tccgggattc
//
LOCUS       DAOPPA010000357         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29514_length_3464_cov_3.326782, whole genome shotgun sequence.
ACCESSION   DAOPPA010000357 DAOPPA010000000
VERSION     DAOPPA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29514_length_3464_cov_3.326782"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PKH54_10470"
     CDS             <1..1022
                     /locus_tag="PKH54_10470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD00361.1"
                     /translation="GIGCGGRKPVDEETVFDLASLTKPLSTALLVAILCDRDAVVLST
                     TLDDFFGADDLGHQLSAGQMSAWRSCDVSALLGHRAGFVPWLPFARELSASLGISAAG
                     SPQAESGVIRMALDSLPVEPSDRIVYSDVGYILLGMILGRVSGRKIAGLFHDLVAGEL
                     GLGSTGFRPIVPVVGSVPSDARFAATAWCGMRGRILQGEVHDDNAWFLGGAAGHAGLF
                     STIFGTASLVDEWIKAIQGRSAILGRDTARRFVFDDFGPRDALRTPGFDRPAAIGSNA
                     GDHAPAGTVGHLGFTGTSFWFDRDSGLSITLLTNRVNPEMHGRKDEIKAMRRAVYDAA
                     WKALA"
     gene            complement(992..2590)
                     /locus_tag="PKH54_10475"
     CDS             complement(992..2590)
                     /locus_tag="PKH54_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00362.1"
                     /translation="MKLKKIAIVRVRGSQDVEFAPSALVTALSPVLGLSRIGRLVKAG
                     KGRTYMPAMALPYIAALCGKADAARGIRREYILVDEPEAGFSGWNWDVDMAMFTVSTS
                     NAPATFRVADRARHFGVKVVMGGIHPSVMPEECLAHADSVARGEAETILGRMLDDYDS
                     GTPAPIYDGGHGTTLEGLPVPAWELGVTPLVKGPGRTRRTGKAVPYAQWVVPVQTSRG
                     CRNACAFCSTTRFQGAARRHRPVAEIADEIRTLKANGVIGPDQTVFFTDNNIVSDSDH
                     RNGIRDTRYARSLFLALKPLGITWVGQGEITVGQDPGLVAMMAESGCHTLLVGLETVS
                     PAGLGRLGKASMKSDTYARSLEVLHDHGISNIGCFIMGLDGHGPETFQPTLDFIERHV
                     DIPQVSVLTPYPGTPLFYQLHRDERILCDDWSLYDITHVVIKPDRLNALDLETAYSRM
                     ISQIYSPLAIVRRAARYARAGGRDGHALKRAGARFTSVLAPNIVYRTLSTTGRGLSGQ
                     IFRGLGATGKNAVSDHASAFQAAS"
     gene            complement(2734..3444)
                     /locus_tag="PKH54_10480"
     CDS             complement(2734..3444)
                     /locus_tag="PKH54_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD00363.1"
                     /translation="MKPQLEKILVIEDDASLAMGLVHNLKFEGFDVLIARDGDKGLAL
                     AFSERPDLVILDVGLPGMNGFDVLAEIRRAGLELRIMMLTARGTDEDKVRGLGLGADD
                     YMTKPFSVRELVARVNALLRRLRLERAEHAARPVTFKDVVLDPASRTAQKAGIPKEMS
                     AREFDLAMFLATHPDHVFSRDQILKAVWGWDYEGTERTVDNFIRAIRASLETDPTDPE
                     FFVTVFGAGYMFRPGGRP"
BASE COUNT          610 a         1040 c         1062 g          752 t
ORIGIN      
        1 ctggcatcgg gtgtggcggg cgcaagcctg ttgacgagga aacggttttc gacctggcgt
       61 ccctgaccaa gcccctttcg acggcgctgc ttgtggcgat tctttgcgat cgcgatgcag
      121 tcgttctttc gaccacgctt gacgactttt tcggcgcgga tgacctgggc caccagttgt
      181 cagccggaca gatgtcggca tggcggtcat gcgacgtttc ggcccttctg ggccatcgtg
      241 ccggattcgt tccatggctt cctttcgcgc gtgaactttc ggccagcctg ggcatttcgg
      301 cggctggaag tccgcaggcc gagtcgggcg ttattcggat ggccctggac tcgctgccgg
      361 tggaaccgtc cgatcgtatc gtctatagcg atgtcggtta tatcctgctg ggcatgatcc
      421 ttggcagggt cagcggccga aaaatcgccg ggctctttca tgaccttgtg gccggggaac
      481 tggggcttgg ttcaaccgga ttcaggccga ttgttcccgt tgtcggttcg gtcccgtccg
      541 atgcccggtt tgcggcaaca gcctggtgcg gaatgcgggg gcggatcctg cagggcgagg
      601 tgcatgacga caatgcctgg ttccttggcg gcgcggccgg ccatgccgga ctgttttcga
      661 cgattttcgg gaccgcatcg ttggtcgatg aatggattaa ggccattcag gggcgatccg
      721 caatccttgg cagggacacg gcaagacggt tcgtgttcga tgatttcggg cctcgggacg
      781 cgcttcgcac acccggtttc gacaggccgg cggccatcgg atcgaacgcg ggcgaccatg
      841 ccccggcagg caccgtcggt caccttggat tcacggggac ctctttctgg tttgatcgag
      901 attccggctt gtcgattacc cttcttacga accgggtaaa cccggaaatg catgggcgca
      961 aggatgaaat caaggccatg cgcagggccg tctatgatgc ggcctggaag gcgcttgcgt
     1021 gatctgaaac ggcgtttttc cccgtggccc caagtcccct gaatatctgg ccggaaaggc
     1081 cgcgtcccgt tgtcgagagc gtgcgataga cgatattcgg tgcaagcacc gatgtgaatc
     1141 ttgccccggc ccttttcagg gcgtgaccgt cccttcctcc ggcgcgggcg taccttgcgg
     1201 cgcgccgaac gatcgcaagc ggcgaataaa tctgcgaaat catgcgtgag tacgcggtct
     1261 ccagatccag cgcgttcagt cgatccggct tgatgacgac gtgcgtgatg tcataaagcg
     1321 accagtcgtc gcaaagaatg cgttcgtccc gatgaagctg gtaaaaaagc ggtgttccgg
     1381 gataaggggt aaggacgctg acctgtggaa tatcgacatg ccgctcgatg aagtccaggg
     1441 tcggctggaa ggtctcgggg ccatgtccgt caaggcccat tatgaagcag ccgatattcg
     1501 atatcccgtg gtcgtgcaga acttcaagcg atcttgcgta agtatctgat ttcatgctcg
     1561 cctttccaag tcgtcccagg cctgccgggc tcaccgtctc aaggccgaca agaagggtgt
     1621 gacaaccgga ctcggccatc atcgcaacaa ggccgggatc ctggccgacc gtgatttccc
     1681 cctgtccgac ccacgtgatt ccaagaggct tcagggccag aaaaaggctg cgcgcatatc
     1741 gggtgtcccg gatgccgttt ctgtggtcgc tgtccgagac gatgttgttg tcggtgaaga
     1801 acacggtctg gtcggggccg atgactccat ttgccttcag cgtccggatt tcgtccgcaa
     1861 tttcggccac cggccggtgg cgccttgccg cgccctggaa cctggtcgtc gagcagaaag
     1921 cgcatgcgtt cctgcagccg cgacttgtct gcaccggcac gacccactgg gcgtatggaa
     1981 ccgcttttcc cgtccgcctg gttcttcccg gacctttcac aaggggggta accccgagtt
     2041 cccacgccgg aaccggcagc ccttcaagcg tggttccgtg cccaccgtca tagattggtg
     2101 caggcgttcc ggagtcgtag tcgtcaagca tccggccaag gatcgtctcg gcttcccccc
     2161 gtgccacaga gtccgcgtgg gcaaggcatt cttccggcat gaccgaaggg tggatgccgc
     2221 ccatgaccac cttcacgcca aagtgccttg cacgatcggc gacacggaaa gtcgccggtg
     2281 cgttcgatgt cgaaacggtg aacatcgcca tatcgacgtc ccagttccag ccggaaaagc
     2341 ctgcctccgg ttcgtcgaca aggatgtact cgcgcctgat tccccgggcg gcatcggcct
     2401 tgccgcacag tgctgcaatg tatggaagcg ccattgccgg catgtacgtc cggccttttc
     2461 cggccttgac cagccttccg atgcgcgaaa gcccgagaac gggcgacagc gccgttacaa
     2521 gcgccgacgg cgcgaattca acgtcctggc tgcccctgac ccttacgatt gcgatttttt
     2581 tcaatttcat gatgacatcc cctgtgcgtg gtggtccttg ccgccacgac cgcgtcaatg
     2641 gcggtcgagt ccatgtacag agtctggatc ttcgatgtca tcgacgtgtc acgggatgtc
     2701 cggattgtgc gcgatggcgg acggatccgc ggcttatggc ctgccgccgg gcctgaacat
     2761 gtatccggca ccgaataccg tcacgaagaa ttcagggtcg gtcgggtcgg tttcaaggct
     2821 ggccctgatt gcccggatga agttatcgac ggtccgttcg gttccctcgt agtcccatcc
     2881 ccagaccgcc ttcaggatct ggtcccttga aaagacgtga tcgggatggg ttgccaggaa
     2941 catcgcgaga tcgaattccc tggccgacat ttccttcggg attccagcct tctgcgcggt
     3001 tcgcgacgcg ggatcaagca cgacgtcctt gaatgtcaca ggtcttgccg cgtgttcggc
     3061 gcgttccagt cgaaggcgcc gcagcagggc gttgacccgg gccacaagct cccgaaccga
     3121 gaacggcttc gtcatgtagt cgtcggcgcc caggccaagt ccgcgcacct tgtcttcgtc
     3181 ggttcccctg gcggtcagca tcatgatccg gagttcaagg ccggcgcgcc tgatttcggc
     3241 caggacgtcg aatccgttca ttcccggaag gcccacgtca aggatcacaa gatcgggacg
     3301 ttcgctgaag gcaagggcca ggcccttgtc accgtcccgg gcgatcagca cgtcgaagcc
     3361 ttcgaacttc aggttgtgga caaggcccat cgccagcgat gcgtcgtcct cgataaccag
     3421 aattttttcg agttgcggtt tcattcatgt tcaccatggc cctg
//
LOCUS       DAOPPA010000358         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29570_length_3460_cov_3.419677, whole genome shotgun sequence.
ACCESSION   DAOPPA010000358 DAOPPA010000000
VERSION     DAOPPA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29570_length_3460_cov_3.419677"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKH54_10485"
     CDS             <1..265
                     /locus_tag="PKH54_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00364.1"
                     /translation="DTFILFDAARPKVSLKPSVLAYLLLDFVPWLKRRGADVGRIDAD
                     FLAGIFADATDMKQKDAAARLAAACDVTLHWTVKPARNGGESK"
     gene            262..1653
                     /gene="gatD"
                     /locus_tag="PKH54_10490"
     CDS             262..1653
                     /gene="gatD"
                     /locus_tag="PKH54_10490"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOD00365.1"
                     /translation="MSTPAERFKGYRGAGRALLERLNVGVWEDVEVKSRLGEFRGLIL
                     PRSETADENHIVLKLDTGYNVGIAVDGIVELKSCGRKEAHYKIPEKEFPFDPAKPNVT
                     LFGTGGTIASRLDYRTGAVIPAFSPGELYGSVPELADVCNLRTEKLYGVFSENMGPEQ
                     YIGLATAVAGEIRKGVKGIVIGHGTDTMHHTAAILSFMVQNSPVPIVMVGSQRSSDRP
                     SSDAALNLIHATRTAGYSDIAETMVCMFGPTSDEYGLLHRGTRVRKMHSSYRSTFRTI
                     GDIPVATVSREKVTPLRGDYKKRRSDTNVILDAVYDNSSTILYYYPGMKPDMVDALVD
                     NGYKGIVIAGTGLGHINKPVYPAIKRAVAAGVHIYMTVQTLWGYVQMYVYDTGRDLME
                     MGIIPCGNMLPEVAFMKLNWALGHSHDREEVKRLMLTPVAGELTPREPPDSYLIYQGG
                     LPDIEKFIKDHKM"
     gene            complement(1810..2679)
                     /gene="dapA"
                     /locus_tag="PKH54_10495"
     CDS             complement(1810..2679)
                     /gene="dapA"
                     /locus_tag="PKH54_10495"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOD00366.1"
                     /translation="MKLEGVITALITPFSSDGTIDFPALRALVEAQIQAGIHGLVACG
                     TTAETPAMKHSESDQVIRAVVDQAAGRVPVIVGTGSNNTDATVEYTRNAKALGADAAL
                     VVTPYYNKPQQEGMKAHFRKVADEGGLPVIIYNIPPRCVVNLTPASILELAADPRFIG
                     VKEAGGSCTQVRDIMMGAPDHFTVLSGDDGLCIPFWALGARGVVSVASNVIPDRMLEL
                     WKLWQSGNVAGAAALDRRLAPLFSALFVETNPAPCKALAEHLGICTSHVRLPLSCATD
                     ATRKAVITAWQSI"
     gene            complement(2709..3326)
                     /locus_tag="PKH54_10500"
     CDS             complement(2709..3326)
                     /locus_tag="PKH54_10500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD00367.1"
                     /translation="MKNSNRVRWSAAVVVFVVALGHFGVQARPVDEVKLQDVIRILQY
                     KPDPTPRDFVECGSDIDSVLADVLSSRNVKPEIRVRAARTLGLYPGTRARAVLTSTMT
                     TPDEDRDVRAASMFGLARLVGGAAIDDIKAWLKDPSPVVRSGAAVALAQIGGPRARQI
                     LMDTIAHEADLDVRLKMDQALNRMSDQESRPMDMRKPGTARSGSN"
BASE COUNT          682 a         1096 c         1048 g          634 t
ORIGIN      
        1 cgacaccttc atccttttcg acgccgcccg accgaaggtt tcactgaagc cgtcggtgct
       61 ggcgtacctg cttcttgact tcgtgccgtg gctgaaacgc cgcggtgccg atgtcggccg
      121 aatcgacgcg gatttcctgg ccggaatctt tgccgacgcg acggacatga agcagaagga
      181 cgcggcggcc aggctggctg ccgcctgcga tgtcacgctg cactggaccg tgaagcccgc
      241 aaggaatggg ggtgaatcca aatgagcacg cctgctgaaa gattcaaggg atatcgtggc
      301 gcgggacgtg cgcttcttga aaggcttaac gtcggcgtct gggaagatgt cgaggtcaag
      361 tcgaggctgg gcgagttccg cggcctgatc ctgccgcgca gcgagactgc cgacgaaaac
      421 cacatcgtcc tgaagctgga caccggttac aacgtaggca tcgcggttga cggcatcgtg
      481 gaactgaagt cgtgcggccg caaggaagcc cactacaaga ttcccgaaaa ggaattcccg
      541 ttcgacccgg ccaagccgaa cgtgacgctt ttcggaacgg gcggcacgat cgcgtcccgc
      601 ctggactacc gaaccggcgc ggtcattccg gcgttttcgc ccggcgagct ttacggctcg
      661 gttcccgaac tggccgacgt ctgcaacctg cggacggaaa agctgtacgg cgtgttttcg
      721 gaaaacatgg ggccggagca gtacatcggc ctggcgacgg cggtggcagg cgaaatccgc
      781 aagggcgtga agggcatcgt catcggtcac ggcacggaca cgatgcacca cacggcggcc
      841 atcctttcgt tcatggttca gaattcgccg gttccgatcg tcatggtcgg cagccagcgc
      901 tcgtcggatc gtccgtcgtc ggacgccgcg ctgaacctga ttcacgcgac gcgcacggcc
      961 gggtattcgg acatcgccga aacgatggtc tgcatgttcg ggccgacttc cgacgaatac
     1021 ggcctgctgc accgcggaac ccgcgtccgc aagatgcatt cgtcgtaccg cagcacgttc
     1081 aggacgatcg gcgacatccc ggttgcgacc gtcagccggg aaaaggtgac ccccctgcgc
     1141 ggcgactaca agaaacgccg gagcgacacc aacgtcatcc tggacgcggt ttacgacaac
     1201 agttcgacca tcctttatta ctatcccggc atgaagcccg acatggtcga tgcactggtc
     1261 gataacggat acaagggcat cgtcattgcc ggtaccgggc ttggccacat caacaagccg
     1321 gtctatccgg ccatcaaaag ggcggttgcc gccggagtgc acatctacat gacggtccag
     1381 accctgtggg gatacgtcca gatgtacgtc tatgacaccg ggcgcgacct gatggaaatg
     1441 ggcatcatcc cgtgcggcaa catgctgccg gaagtcgcgt tcatgaagct gaactgggcg
     1501 cttggccaca gccacgaccg cgaggaagtc aaaaggctga tgctgacgcc tgtcgccggc
     1561 gaactgaccc cgcgcgaacc accggacagt tacctgattt accagggcgg cctgcccgac
     1621 atcgaaaagt tcattaaaga ccacaagatg taacctgccc gaagagcggg ccgttacccc
     1681 ggaacacgcc ctgcgaagca cgccctgccc cgggcaccag aaacgaaggc gaatcccaca
     1741 aataattgaa cagttccgat caatccggaa tctgccccga aaaatcgcgt cgaatcgaaa
     1801 ttgcttgaat cagattgact gccaggcggt tatcacggcc ttacgggtgg cgtccgtggc
     1861 gcaggaaagc ggcaggcgga cgtgtgacgt gcagattcca aggtgctcgg ccagggcctt
     1921 gcacggtgcc ggattggttt cgacgaaaag cgcggaaaaa agcggcgcaa gacgccggtc
     1981 aagcgcggcc gcgccggcca cgttgccgga ctgccacagc ttccacagtt caagcatccg
     2041 atccggaatg acgttggacg cgaccgacac gaccccgcgc gccccaagcg cccagaacgg
     2101 gatgcacagg ccgtcgtcgc cggaaagcac ggtgaagtga tccggcgcgc ccatcatgat
     2161 gtcgcgaacc tgggtgcacg acccgcccgc ttccttgacg ccgatgaatc gcggatccgc
     2221 ggcaagctcc aggatcgacg ccggcgtcag gttgacgacg cagcgcggcg ggatgttgta
     2281 aatgatcacg ggcagcccgc cttcgtcggc gaccttgcgg aagtgggcct tcatgccttc
     2341 ctgctgcggc ttgttgtagt acggggtcac gaccagggcg gcgtcggcgc caagcgcctt
     2401 tgcattcctg gtgtattcaa cggtggcgtc ggtgttgttg ctgccggttc cgacaatgac
     2461 aggcacgcga cccgcggcct ggtcaacaac ggcacgaatc acctggtcgc tttcggaatg
     2521 cttcatcgcg ggcgtttccg cggtcgtgcc gcatgcgacc aggccatgga tgccggcctg
     2581 gatctgggcc tcgaccaggg cacgcaaggc cgggaaatca atcgtcccgt cgcttgaaaa
     2641 cggcgtgatc aacgcggtga tgacaccttc aagtttcatg gcaacctcca cttggtgggc
     2701 cgaccgggtc aattcgagcc ggaccttgcg gtaccgggct ttctcatgtc cattggccgg
     2761 gactcctgat ccgacatccg gttgagtgcc tgatccatct tcaggcgaac gtccagatcg
     2821 gcttcatgcg caatcgtatc catcaaaatc tggcgcgccc gcggcccgcc aatctgggcc
     2881 agggctaccg cggccccgga ccttaccacc ggggacggat ccttcagcca tgccttgata
     2941 tcgtcgattg ccgccccgcc gaccaggcgg gccagcccga acatgcttgc cgcccgaaca
     3001 tcgcggtctt catccggcgt cgtcatcgtc gaggtcagga cggccctggc ccgggtgccc
     3061 ggatacaggc ccagtgtcct tgcggccctg accctgattt ccggcttcac gtttcgtgac
     3121 gaaagcacgt cggcaagcac cgaatcgatg tcggatccgc attcgacaaa atcgcgcggg
     3181 gtcgggtccg gcttgtactg aagaatcctg attacgtcct gcagcttcac ttcatcaacc
     3241 gggcgcgcct gaaccccgaa atggccaagg gcaaccacga aaaccacgac tgccgccgac
     3301 catcggaccc ggtttgaatt cttcatttcc tgctcttctt ccttgccttg cgggcttcct
     3361 tttccttctt gcggcgcttg tccttgtcgg cctgggaagt cttgcgcgga cctgcgccgt
     3421 aaggattcgc gtaccccttc ggcatcttgc cgccgcccat
//
LOCUS       DAOPPA010000359         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29718_length_3448_cov_3.680224, whole genome shotgun sequence.
ACCESSION   DAOPPA010000359 DAOPPA010000000
VERSION     DAOPPA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29718_length_3448_cov_3.680224"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..866)
                     /locus_tag="PKH54_10505"
     CDS             complement(144..866)
                     /locus_tag="PKH54_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOD00368.1"
                     /translation="MKALISALRALQDVDQHRIDAEKERQSIASRVGSLAEMVSELER
                     EQADKRDKLREAERWYRERDRELKEDNDKIKRAQTRLNALSKQKEHAAVQREIETLRR
                     SNQQKEEEILKLVNVMEEFKAAVDEEEKKLESMRQDLAREEASSVERISELDGRIAEL
                     GNNYKDYEANIPGDVLRQYKRIQKAWLGMAVVPVDAKGSCGGCHRRLPPQLYNTLLRN
                     DALHTCPYCNRFVFVEPQEAAE"
     gene            complement(863..>1098)
                     /locus_tag="PKH54_10510"
     CDS             complement(863..>1098)
                     /locus_tag="PKH54_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00369.1"
                     /translation="GSSPGGPTRYRVVAMGLVNNTSLKNMARRRPAAMHKATFWIPGK
                     QGAPLRAPFLYSGSPDHTPIPDFLPITVLEVSL"
     gene            complement(1072..1161)
                     /locus_tag="PKH54_10515"
     tRNA            complement(1072..1161)
                     /locus_tag="PKH54_10515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1126..1128),aa:Ile,seq:cat)
     assembly_gap    1099..1108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1291..2370)
                     /locus_tag="PKH54_10520"
     CDS             complement(1291..2370)
                     /locus_tag="PKH54_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00370.1"
                     /translation="MSGFEARTTELLETLSRYQGKRLFVAVIGSPDPDGLASAWTLKT
                     IARYAGVTMDILTFEVLSRPDNIAFCQMLAIPFKHVEALPRVGYAGYCVVDRQNARLP
                     IGYSRKLPLIAHIDHHVEIPTGAIFAQQDPGFGSCCSIMAHHLKYLCDHFETDQSEVC
                     RLATALMFGIRTDTQNFLTAKSVDYESAAILARHFSIELIRTLIDTPVGRPFINTLAS
                     ALESRQSRAGLVIAWAGTVARSGRDTIGQTADFLIRGDKTVAVIVYGCVEDYLVGSMR
                     TTDPDLETFRFIDESLSRIFQFQIDCGGRTFSGGFQIPLKFIANDDMNSVRKQIDQSI
                     FHVWGLYNKHPVGSAGWKKRRISTI"
     gene            complement(2488..3249)
                     /gene="grpE"
                     /locus_tag="PKH54_10525"
     CDS             complement(2488..3249)
                     /gene="grpE"
                     /locus_tag="PKH54_10525"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD00371.1"
                     /translation="MDSREKQDLINEFGPVDGIDVGDGPAEVTQPGTGASLEFEFDDV
                     DSTKESESDDQHVHAMDGAMESDDASEPGPTERKELQSDLVFDFIGTDSPVPKDQIGL
                     DPVSAELAETKDKLLRLAAEFENFKKRRIRERDEQQKYANERVLKELLPVIDNLERAL
                     DSARQAGQAANLITGLDLVIHEFLRVLSREGVEPVLAVGRPFDPAYQEALQSLETTES
                     DPGTVVAEILRGYTLHGRVLRAGLVVVAREPQNGR"
BASE COUNT          701 a          928 c          969 g          840 t
ORIGIN      
        1 cttccggtcg agatgttcgt tgccgacgtc gatgccgacg gttgcctgga catagtcacg
       61 gccgaccaga ccggaaagac ccttaccagt cttcgaaaca tgaccgctac atgccgtaat
      121 ttctgatcag accgccagtc ggttcattcg gcggcttcct gtggctcgac aaaaacaaag
      181 cgattgcagt acgggcaggt gtgaagcgcg tcattgcgca gaagcgtgtt gtaaagctgg
      241 ggcggcagcc tgcggtgaca gccgccgcaa ctgcccttgg catcgacagg aacgacggcc
      301 atgccaagcc acgccttctg gatgcgtttg tactggcgaa ggacgtcgcc tgggatgttg
      361 gcctcatagt ccttgtaatt attgccaagt tcggcaatgc gcccgtccag ttccgatatc
      421 ctttcaaccg aggaagcctc ttcgcgggcc agatcctggc gcattgattc gagctttttt
      481 tcctcttcgt caacggccgc cttgaattct tccatgacgt tgacaagctt caggatttct
      541 tcttccttct gctggttgct tctgcgaaga gtttcgattt cgcgctgcac ggccgcgtgt
      601 tccttctgct tgctcagggc gttcaaccga gtctgggcac gcttgatctt gtcgttgtct
      661 tccttcagtt cccgatcgcg ttcacggtac cagcgttcag cctcgcgaag cttgtcgcgc
      721 ttgtcggcct gttccctttc aagttccgaa accatttcgg caagagatcc aaccctgctg
      781 gcaatggact ggcgttcctt ttcggcgtcg attcggtgct gatcgacgtc ctggagcgca
      841 cgaagggcgg aaatgagtgc tttcaaagtg aaacctccag tactgttatg ggaaggaaat
      901 caggaatggg cgtgtggtct ggactcccgg aatacaaaaa aggcgcccgc agtggagcgc
      961 cctgttttcc gggaatccag aaggtggcct tgtgcatcgc tgccggccgc cttcgggcca
     1021 tgtttttcag tgaggtgttg ttgaccaacc ccatcgccac cacacggtat ctggtgggcc
     1081 cacctgggct cgaaccagnn nnnnnnnncc agggaccaac cggttatgag ccggtggctc
     1141 taccaactga gctataggcc caaggcccaa aaagcggtgg aagtatagat tagcgggccg
     1201 gtgtttgcaa tctatatttg ggcgtggcgg tgaaataatc gacagccgcc tggtgccgga
     1261 gtgttgatgg gcggtgaaac ggccatttgt tcagatggtt gagatgcgcc tcttcttcca
     1321 gccggcgctt ccaaccgggt gcttgttgta tagcccccag acatggaata ttgattggtc
     1381 aatctgcttt ctgacagagt tcatgtcatc gttggcgatg aacttcaggg gaatctgaaa
     1441 gccccctgaa aaagtcctgc cgccgcagtc gatctggaat tggaagatcc tggaaagcga
     1501 ttcgtctata aaccggaatg tctcaagatc cggatcggta gtcctcattg atccgacaag
     1561 ataatcttcg acgcatccat acacgattac ggcaacggtc ttgtccccgc ggatgaggaa
     1621 atcggcagtc tgtccaattg tgtcgcgtcc gctgcgtgca accgttccgg cccacgcaat
     1681 taccagcccg gcccggctct gtcgtgattc cagggccgat gccagggtgt tgatgaacgg
     1741 tcggccgacc ggcgtatcaa tcagggttct tatcaattct attgagaagt gcctggccag
     1801 tattgcggct gattcgtaat cgacagattt ggcggtaagg aaattttgcg tgtcggtcct
     1861 gatcccgaac atcaaggccg tggcaaggcg acatacttcc gactggtcgg tttcaaagtg
     1921 gtcgcacagg tatttcaagt ggtgggccat gatactgcag caggaaccga atcccggatc
     1981 ctgctgggcg aagatcgccc cggtcgggat ttccacatga tggtcgatat gcgcaatcag
     2041 gggcaatttc cttgaatacc ctatcggaag tcttgcgttc tggcgatcga ccacgcagta
     2101 acctgcgtat ccgacccttg gaagggcttc gacatgtttg aacgggatcg ccagcatctg
     2161 gcagaaggcg atgttgtccg gccgtgaaag gacttcgaat gtcaggatat ccatggtgac
     2221 gccggcatat cgggcgatag tcttcagcgt ccaggccgat gccagtccat ccggatccgg
     2281 cgagccgatg actgcgacga agagccgctt gccctgatat ctggacaggg tttcgagcag
     2341 ttcagtggtt cgggcttcga atccgctcac ggaaggctcc ttgcctgggg tcgcatctac
     2401 gtgacaatgg tagattctat catcagtcca tggctttcaa cagggtaact taattaagtc
     2461 ggattaaggt gttacagcgg taggtcgtca gcggccgttt tgcggttccc ttgcaaccac
     2521 aaccagaccg gcccgcagga cgcggccgtg cagcgtgtat ccgcgaagga tttcggccac
     2581 caccgtgccg gggtcggatt cggtggtttc aagggattga agggcctcct gataggcggg
     2641 atcgaatggc ctgccaacgg caagaactgg ttcaacacct tcacgtgaaa gtacccgcag
     2701 aaattcatgg attaccagat caaggccggt tatgaggttg gcggcttgtc cggcctgcct
     2761 ggccgagtca agtgcccttt ccaggttatc gataacgggc aggagctctt tcagtactct
     2821 ttcgtttgcg tatttctgct gttcatcacg ttcacggatg cgacgttttt tgaagttttc
     2881 aaactctgcc gcaagccgaa gaagcttgtc cttggtttcg gcaagttcgg cgctgacggg
     2941 atcaagcccg atctgatcct ttggcaccgg tgaatcggtg ccgatgaaat caaaaacgag
     3001 atccgactga agttctttcc tttccgtcgg cccgggttca ctggcatcat ccgattccat
     3061 cgcaccatcc atcgcgtgga catgctggtc atcggattcg ctttcctttg tggaatcgac
     3121 atcatcaaat tcgaattcaa ggctggcgcc ggttcccggc tgtgtcacct cggctggccc
     3181 gtcgccgaca tcaatgccat cgacagggcc gaattcatta atcaggtcct gcttttctct
     3241 ggagtccatg aactaacctc aaggcaaatc tcgcgggggc cctggccgaa cccgctatag
     3301 ttttatggtc tgcttccgga tttgtcaacg caagccggct caaatgcttc aaacccgatt
     3361 gtaaggtgtg taaaatatcg cgccgcgtca tgtatgggcg gtcaattccg gaggagtgat
     3421 gattcgcaat cggttaatca aggcgccc
//
LOCUS       DAOPPA010000360         3441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29793_length_3441_cov_3.407265, whole genome shotgun sequence.
ACCESSION   DAOPPA010000360 DAOPPA010000000
VERSION     DAOPPA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29793_length_3441_cov_3.407265"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..705
                     /gene="thiE"
                     /locus_tag="PKH54_10530"
     CDS             64..705
                     /gene="thiE"
                     /locus_tag="PKH54_10530"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOD00372.1"
                     /translation="MSGINLSVYLVTDPVLKTPGLEVCEIVDRACSAGVSAVQYRDKD
                     CPLEQYILTARKLKCITSRHGVPLILNDHVTAVRSVGADGIHVGQDDMSAAQVRKLVD
                     PDCIVGVSVFDAHQARQAENDGATYVAVNGVFPTGTKHFADSAGLGLARVTEICRAVH
                     IPVVGIGGINVFNAAAVIAAGASGVAVVTAITMRPDIEDAVRELLLETSGVKR"
     gene            846..1652
                     /locus_tag="PKH54_10535"
     CDS             846..1652
                     /locus_tag="PKH54_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD00373.1"
                     /translation="MAVRKIVSIDEELCDGCGLCVPSCAEGAIRIIDGKARLVGDNLC
                     DGLGACLGECPQGAITVTEREAAEFDEEAVRLARAAGDAAGGHRDEPIGAGVKPQVAS
                     THGVADGHHHGGGCPGSRLHSFARTGTPAVGDVAGQVPSRLGQWPVQLHLLPPTAPFL
                     RDSDLVLAADCAAFAFGDFHNRFIRGRSLAIACPKLDSGLDEYLQKLTGMIDHGGINT
                     LTVVIMEVPCCGGLLHLAMRAVQAAERKIPLKMVKISLKGDILDERWVQV"
     gene            complement(1653..2762)
                     /locus_tag="PKH54_10540"
     CDS             complement(1653..2762)
                     /locus_tag="PKH54_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD00374.1"
                     /translation="MIDRRRRQTILIAEDTPANIDILIEALADRYDISVAIDGETVLN
                     SVRRINPDLILLDVVMPGIDGYEVCRRLKEDQATKNIPVIFLTSLSLTDNEERGLNLG
                     AVDYITRPFNPRIVEARVHTHLELNRYRVHLENIVAERTADLNATREATIQSMAILAE
                     FRDTDTGGHIRRTREYVRILLTYLHARGRHRDELTHKSIEQIAQSAPLHDIGKVGLPD
                     RILRKPSELSPEEYEEMKRHPLYGGEAIRRAEESLGENSFLRYAREIAESHHENWDGS
                     GYPLGLAGEQIPISARAMAVADVYDALVSARAYKQGMSHEEALRMIREKSGTKFDPEM
                     VEALVATQHEFHKVSERFKDGEVETASPEGTPDAH"
     gene            complement(2759..>3441)
                     /locus_tag="PKH54_10545"
     CDS             complement(2759..>3441)
                     /locus_tag="PKH54_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOD00375.1"
                     /translation="IDLQPPRFQHDIDVPGLNVGTGLSYVGGNINLYRKLLRKFVEEY
                     HGRDACLEPAALNNNPEESFRFVHSVKGLAGTLGATGLQDKAAVLEKALHDRDYFQSG
                     LIEEFRSMFTQLLSDIRTRTGLFDPVEPAAQATAAPVRKSSEDPARVFASLSESLELL
                     QPRQSARYIATLMTMEWPSDVGLQLGRVQDEIRNYRLTAALAMLKALEPKLATQQGTS
                     AAAGENAR"
BASE COUNT          615 a          998 c         1051 g          777 t
ORIGIN      
        1 gcgagatttc ctctggtcgg tcgaagagga acaggttcgc gccggcgtga aggtcaggcg
       61 cccatgagcg ggatcaatct ttctgtttat ctggtcaccg atccggtact gaagacgccg
      121 ggcctggaag tctgcgaaat cgttgacagg gcctgctccg ctggggtttc cgcagtccag
      181 taccgcgaca aggattgtcc gctggaacaa tacatcctga cggcccggaa gttgaagtgc
      241 attacgtccc gacacggcgt tccactgatt ctgaacgatc acgtcacggc ggtccgttca
      301 gtcggtgccg acgggatcca tgtcggccag gatgacatgt cggcggcaca ggtcaggaaa
      361 ctggtcgatc ccgactgcat cgtcggggtg tcggtcttcg acgcacatca ggcgcggcag
      421 gctgaaaacg acggggccac ctatgtcgcc gtcaatggcg tatttccaac cggtaccaag
      481 catttcgcgg attctgcggg tcttggcctg gcccgggtga ccgaaatatg tcgggcagtc
      541 catattccgg tggttggaat cggtggaatc aacgtcttca acgccgccgc cgtcatcgcg
      601 gccggcgctt ccggagttgc cgtcgtgacc gccatcacca tgcgtcccga catcgaggac
      661 gcggttcggg aacttctgct tgaaacgagt ggtgtgaaga gataagtctt taattttgtt
      721 ggcctgacgt ctaacaaaga taatttttgt cctattttac tttccttctg ctataatcgc
      781 atccggtcag ccggtctggc ctttgaaacc gggcatcaag gcaaattcgc gcttggagaa
      841 tgatcatggc ggtaagaaag attgtcagca ttgacgagga actgtgtgac ggatgcgggc
      901 tgtgcgtgcc gtcgtgcgcc gagggtgcga tacgcatcat cgacgggaag gcaaggctgg
      961 tcggggataa cctttgtgac ggtcttggag cctgtctcgg ggagtgtccg cagggcgcga
     1021 taaccgttac cgagcgcgag gccgccgaat ttgacgagga ggccgtccgc ctggcaaggg
     1081 ccgccgggga cgctgccggc gggcaccgcg atgagccgat tggcgctgga gtcaagcccc
     1141 aggtcgcttc cacgcacggc gtggccgacg gtcaccacca tggcggtggc tgtcccggtt
     1201 cccggcttca cagttttgca cgcacgggca cgccggcagt cggtgacgtg gccggccagg
     1261 ttccgtcgcg gctcggccag tggccggttc agcttcatct tcttcccccg accgcgccct
     1321 tcctgcggga cagcgatctt gttcttgccg cggactgcgc ggcgttcgcg tttggcgatt
     1381 ttcacaaccg gtttattcgc ggccgttcgc tggcgatagc ctgtcccaag cttgacagcg
     1441 gcctggatga gtaccttcag aagttgaccg gcatgattga ccacggcggc atcaatacgt
     1501 tgacggtcgt catcatggag gttccctgct gcggcggact tttgcacctg gccatgcggg
     1561 ccgtccaggc ggcggaaaga aagattccgt tgaaaatggt caagatcagc ctgaagggcg
     1621 acatccttga cgaaaggtgg gttcaggtct gatcagtgcg cgtctggcgt cccttccggc
     1681 gacgccgtct caacttcccc atccttgaac ctctccgaaa ccttgtggaa ttcgtgctgc
     1741 gtcgcgacaa gggcctcgac catttccggg tcgaacttgg ttccgctctt ttcccggatc
     1801 atccgaaggg cctcttcgtg cgacatgccc tgtttatagg cgcgggcgct gacaagtgcg
     1861 tcgtatacgt ccgcaaccgc catggcgcgt gccgatatcg ggatctgttc gcccgccagt
     1921 ccaaggggat aaccggatcc atcccagttt tcgtggtggc tttccgcaat ttcacgggcg
     1981 tatcgcagga acgagttttc acccagcgat tcctcggcgc gcctgatggc ttccccgcca
     2041 tacagaggat gtcgcttcat ttcctcgtat tcctcggggc tgagctcgga aggcttgcga
     2101 aggattctgt ccggcaaccc gacctttccg atgtcgtgca gtggtgccga ctgggcaatc
     2161 tgttcgatcg acttgtgggt cagttcgtcc cgatgacgtc cccttgcgtg gagataagtc
     2221 agaagaatcc gaacatattc cctggttctg cggatgtgcc cgccggtgtc ggtgtccctg
     2281 aactccgcaa ggattgccat gctctggatg gtcgcttcac gggtcgcgtt caggtcggct
     2341 gtccgttcgg ccacgatgtt ttccaggtgt acccggtatc ggttcagttc caggtgggta
     2401 tgcacccggg cctcgacaat ccttgggttg aacgggcggg tgatgtaatc gaccgcccca
     2461 aggttcaggc cgcgctcctc gttgtccgta agtgacaggg atgtcaggaa gatcaccggt
     2521 atgttcttcg tggcctggtc ttccttcaac ctgcggcaga cttcgtatcc gtcgatcccg
     2581 ggcatcacga cgtcaagcag tatcaggtcc gggttaatcc tgcgcactga attcagaacc
     2641 gtttcgccat cgatggcaac gctgatgtcg taccgatccg ccagtgcctc gataaggatg
     2701 tcgatattgg ccggagtgtc ttccgcaatc agaatggtct ggcgtcttcg gcggtcaatc
     2761 atcttgcgtt ttcccccgcg gccgcggacg tgccctgctg tgtcgcaagt ttcggttcca
     2821 gagccttcag cattgcaaga gcggcggtca ggcgatagtt gcggatttcg tcctgtaccc
     2881 gtccaagttg caggccgaca tcggaaggcc actccatcgt catcagcgtg gcgatgtacc
     2941 tggcggactg acgaggctgc agcagttcaa gcgattccga cagggacgcg aacaccctgg
     3001 ccggatcctc gcttgatttc ctgaccggcg ccgccgtcgc ctgcgccgcc ggctcgaccg
     3061 ggtcaaagag ccccgtcctg gttctgatat ccgacagaag ctgggtgaac atggaacgga
     3121 attcctctat caggccgctc tggaagtagt ccctgtcatg aagcgccttt tcaagaacag
     3181 ccgccttatc ctgcaggccg gtggctccaa gcgttcccgc caggcccttg accgaatgga
     3241 caaacctgaa ggattcctcc ggattgttgt taagagcggc cggctccagg cacgcatcgc
     3301 gtccgtggta ttcctcgacg aatttgcgaa ggagtttgcg gtaaaggtta atgttgccgc
     3361 ccacgtaact gagtccggtt ccaacgttca ggccgggaac gtcaatatcg tgctggaatc
     3421 gcggcggctg aagatcgatc g
//
LOCUS       DAOPPA010000361         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29805_length_3439_cov_4.274823, whole genome shotgun sequence.
ACCESSION   DAOPPA010000361 DAOPPA010000000
VERSION     DAOPPA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29805_length_3439_cov_4.274823"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..952)
                     /gene="pdxJ"
                     /locus_tag="PKH54_10550"
     CDS             complement(209..952)
                     /gene="pdxJ"
                     /locus_tag="PKH54_10550"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016402851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD00376.1"
                     /translation="MKLNRIRLGINVDHIATLRQSRGTKYPDPVLAAGLAELAGADQI
                     TIHLREDRRHIQDRDVRILRQTIGTIMNLEMAANEDVMAIACEVRPDLVTLVPEKRAE
                     LTTEGGLAVVGREREVAATINRLNKSGIRVSLFINPDPDEVRASVECGAEQIEIHTGF
                     YADANNPARAVAELERIRAAAAMGGKLGVTVAAGHGLDYVNTRPVCHIPEIEELNIGH
                     SIMARAFFVGLDQAVREMIQLIREEEGQN"
     gene            complement(1031..2398)
                     /gene="glmM"
                     /locus_tag="PKH54_10555"
     CDS             complement(1031..2398)
                     /gene="glmM"
                     /locus_tag="PKH54_10555"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD00377.1"
                     /translation="MASIERELFGTDGIRGIANVHPLTPEVALALGRAAAVMFPGGAG
                     RTRILIGKDTRISGYMFEDALAAGICSMGADVLLVGPIPTPGIAYLTIGNRADAGVVI
                     SASHNPFMDNGIKFFGRDGFKLPDEIELRMEEYVLGRAKLPPVATGKDFGRASRIEDA
                     VGRYCVYLKSQFPRDRSLEGMRIGLDCANGAGYRVAPLVFSELGAEVVTIGDKPDGTN
                     INEACGALHPKALGRKVREMRCDVGLALDGDGDRAMLIDENGEIVNGDGVMAICSDAM
                     HRAGLLKGGGIVGTTMSNLGLELALERNGLHLVRTDVGDRYVVERMLADGYNLGGEQS
                     GHLLFLNHATTGDGILSGLMILEVMLKTGKRLSELAKIYEQFPQVNYSRAVVDRPPIE
                     TLAQTNEAIRTVEAALGKEGRVVVRYSGTSKKIRVMVEGRDEGMVQTYAEQILAAAGR
                     DEILA"
     gene            complement(2447..>3439)
                     /locus_tag="PKH54_10560"
     CDS             complement(2447..>3439)
                     /locus_tag="PKH54_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOD00378.1"
                     /translation="AFPRKRPPQGWLIRIFRENVGIKVLSLILAIVFFALVRTEQGNE
                     AEIEIPVQLSALSEDLVFVGEMPRKIKVLVRYKWSRPKKDTQPPPYIVDLRGFENEKV
                     FVFDAEKIRLAMNTEGMSIVSIYPPEFTVEVEPKVEKTVKVKLNLVGISEKGYDVVVE
                     EARSLPPVIKVRGAKSAVKDIDIFATHPIDISKFKKDVILDNVPLQKPSSKFLFMETD
                     TVRVEIPVREIPGQKMLDNVEVKVRNCPEGFMCIVTPPLVNMTLTGPLPSIFLVENGQ
                     EKAEVWVDAALIDAKVDKHQGIKLSCDRPMGLKCVETPKSVTLTIQKNPAEPSR"
BASE COUNT          605 a         1047 c         1004 g          783 t
ORIGIN      
        1 cctactatcc gactgctatg tccagatttc ttcagttata aacgttcaac gacaacggaa
       61 gggaatgtct ggatcttgag gttttatgtg cggtcggcgc gttgatggga acctttgatg
      121 ttgaggccgt ctgtccaggc ggcgagctgt cggcggaaat gccccccatg gtgtgtgcgg
      181 aactggacga aatttttgat ggattgtttt agttctgacc ttcctcttcc ctgatcagct
      241 ggatcatttc acggacggcc tggtcgaggc cgacgaagaa ggcgcgggcc atgatggagt
      301 ggccgatgtt cagttcttca atttcgggga tgtggcagac ggggcgggtg ttgacgtaat
      361 ccaggccgtg gccggcggcg acggtaacgc ccagtttgcc gcccatggcg gcagcggcgc
      421 ggatgcgttc aagctcggca accgcgcggg cgggattatt ggcgtcggcg taaaagccgg
      481 tgtggatttc gatctgctcg gcgccgcatt cgacggacgc gcggacttcg tccgggtcag
      541 ggttgatgaa caggctgacc ctgatgccgg atttgttcag gcgattgatg gtcgcggcga
      601 cttcgcgttc gcggccgacg acggccaggc cgccttcggt ggtcagttcg gcgcgtttct
      661 ccgggaccag cgtcaccagg tcggggcgga cttcgcacgc gatggccatc acgtcttcgt
      721 tggcggccat ttccaggttc atgatcgtgc cgatggtctg gcgcaggatg cgcacgtcgc
      781 gatcctggat gtggcggcgg tcttcacgca gatggatggt gatctggtcg gccccggcca
      841 gttcggccag gccggcggcc agcaccggat cggggtattt cgtgccgcgc gactgtcgga
      901 gcgtcgcgat gtgatcaacg tttatgccaa gacggattcg attcaatttc atcaccacag
      961 cgggcggatt ttcatccgcc catacaagcc acagcgggcg gattttcatc cgcccataca
     1021 agcccatttg tcacgccagg atttcgtcgc ggccggcggc cgccaggatc tgttccgcgt
     1081 acgtctggac catgccttcg tcgcggccct cgaccatcac gcgaatcttc ttcgacgtgc
     1141 cggaatacct gactaccacg cgaccttcct tgccaagcgc cgcttcgacc gtgcggatcg
     1201 cttcgttcgt ctgggccagg gtttcgatcg gcggccggtc aaccacggcg cggctgtaat
     1261 taacctgcgg gaactgttcg taaatttttg ccagttcgct taaccgcttt ccggtcttca
     1321 acattacttc caggatcatc agccccgaca ggatgccgtc gcccgtggtc gcgtggttca
     1381 gaaacagcag gtgacccgac tgttcgccgc ccaggttgta tccgtcggcc agcatccgtt
     1441 cgaccacgta cctgtcgccg acgtcggtcc ggaccaggtg cagcccgttt ctttccagcg
     1501 ccagttccag gcccaggttc gacatcgtcg tgcccacgat cccgccgccc ttcagcagcc
     1561 cggcccggtg catcgcgtcc gaacatatcg ccatcacgcc gtcaccgttt acgatttcgc
     1621 cgttttcatc gatcagcatc gcgcggtcgc cgtcgccgtc cagcgccagt ccgacatcgc
     1681 agcgcatttc gcgcaccttg cggcccagcg ccttcggatg aagcgcgccg cacgcctcgt
     1741 tgatgttggt gccgtccggc ttgtcgccga tggtgaccac ttccgcgccc agttccgaaa
     1801 aaacaagcgg agcgacccgg tatccggcgc cgttcgcgca gtccaggccg atcctcatgc
     1861 cttcaagcga tctgtcacgc gggaactgcg acttcaggta cacgcagtaa cgcccgaccg
     1921 cgtcctcgat tctgctggcg cgaccaaagt ccttgcccgt ggcgaccggt ggcagtttgg
     1981 cccggcccag cacgtattct tccatcctca gctcgatttc gtcgggcagc ttgaatccgt
     2041 cgcgcccgaa gaatttgatg ccgttgtcca tgaacgggtt gtgcgaggcc gaaatgacca
     2101 cgcctgcgtc cgcacggtta ccgatcgtca ggtacgcgat gcccggggtc gggatcggtc
     2161 cgaccagcag cacgtcggcg cccatcgagc agatgcccgc ggccagggcg tcctcgaaca
     2221 tgtacccgga aatgcgggta tccttgccga taaggatgcg ggttcgtcct gcaccccccg
     2281 gaaacatgac tgcggccgcc ctgccgagcg cgagtgccac ttcgggagtc agtggatgca
     2341 cgttggcgat tcccctgatt ccgtcggtac cgaaaagttc tctttcaatg ctggccataa
     2401 gtgcctccgg ggctggtcct gcgaagcgga ccgaatttta cacctttcac ctggacggtt
     2461 cagcgggatt cttctggatc gtcagggtta ccgatttcgg ggtttcgacg cacttcagac
     2521 ccatcggccg gtcacaggaa agcttgattc cctgatgctt gtccactttc gcgtcaatca
     2581 gtgccgcgtc gacccagacc tctgcctttt cctgaccgtt ttcgaccagg aagatggacg
     2641 gaagcgggcc ggtaagcgtc atgttcacga gtgggggggt gactatgcac atgaagcctt
     2701 ccggacagtt gcgcaccttg acttcgacgt tatcgagcat tttctggccc ggaatttccc
     2761 gtaccgggat ttcgacccgt accgtgtcgg tttccatgaa aaggaacttc gaagacggct
     2821 tctgcagcgg cacgttgtcc aggatgacgt cttttttaaa cttgctgatg tcgatcgggt
     2881 gagtggcaaa gatgtcgatg tccttgaccg ccgatttcgc gcccctgacc ttgatgacag
     2941 gcggaagcga acgggcttcc tcgacgacga cgtcataccc cttttcggaa atacctacca
     3001 ggttcagctt gaccttgact gttttttcaa ccttgggctc cacctcgacc gtgaattccg
     3061 gcgggtagat tgaaacgatc gacattcctt ccgtgttcat cgcaagcctg atcttttcgg
     3121 catcgaaaac aaagaccttt tcgttttcaa agccgcgcag atcgacgatg tatggtggtg
     3181 gctgggtgtc ctttttcggc ctggaccact tgtacctgac cagcactttg atttttctgg
     3241 gcatctcgcc cacgaaaacc agatcctccg acagggcgct caactgcacc ggaatctcga
     3301 tttcagcttc gtttccctgt tcggtcctga ccagcgcgaa aaatacaatc gccaggatca
     3361 gcgacagcac cttgataccg acgttttcac gaaaaatccg gattaaccat ccctggggcg
     3421 ggcgcttgcg cgggaatgc
//
LOCUS       DAOPPA010000362         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_29985_length_3424_cov_3.285545, whole genome shotgun sequence.
ACCESSION   DAOPPA010000362 DAOPPA010000000
VERSION     DAOPPA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29985_length_3424_cov_3.285545"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PKH54_10565"
     CDS             complement(<1..1816)
                     /locus_tag="PKH54_10565"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD00379.1"
                     /translation="MKRTIPPIVMVPIAAVAATVTGLAIIFPIQWLMVAAIVAGLTVA
                     GGAAVLVAGWALSRRAVGESVPVVKGHDRFVIVTVAATAVLWALCAPDVYFRDGGELA
                     GAAVGLGVPHPTGFPAFCMAGKLFSFIPAGTAFFRINLLSGLAAALAAAMSWLLADRV
                     TRRSESIPAALWWVAPLAFLGSANAWLHATTTEVYSISMLGLAAVVFLGLESVQRRDA
                     RLLAAAASIAGIGAGVHITMPLYGSFVILACLVGLARCGACGPDRLRGLFRTGFLVMM
                     AGFIGTFVVLYLPVAAGRDPLLNWGDPSSLDRFVAHLSGQRIRDSFGGRIGGSGIVEI
                     VANATMAGRNLVESLWPILPLAISGVVLGGRRRPLAVVILSFLILADFSFAVFINPMG
                     IFDLQVLMTATWATAVLAGIGASEIAFAMIGRAGTAGWLATGGLAALLVVFQWISAPA
                     DRDMSGLHGAREITDHVVRDYPWGSIVMTASDDLSSILLGRQAVEDSRPDLAILVKQH
                     LSDHAFVTRRLNPLRLAFPAGGRASILDAVDARPFESVGETASDGLARAVRTFMPLAP
                     VFLEPGEGRVDESVIDLFQPAFPLWVAAAPVASGPGGSP"
     gene            complement(1835..>3424)
                     /locus_tag="PKH54_10570"
     CDS             complement(1835..>3424)
                     /locus_tag="PKH54_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00380.1"
                     /translation="SKGVRGGMRVTKQGTGDAFYYIPVNSIGLFNRLALPDRRLNPEP
                     GDPVVTCLTDADCRRVIPCEDPDKPEVCPCPVTVECEDWEIDQGDCPDGTKVMQYNVH
                     PGALCLDGTCRLESLFPLNTRGDRAFAASVSTSGGDVVTVGGFSFLDAGRMKVAGYTD
                     EVLAPETFFMDGGTGLFEILEHDRALDAGLGLASAAMIDATRLVVVGGTSTAGTGAIE
                     GVVASPVIESQECVDSCPVEYSPYMYLIDTTTGAVSRATMETPIIASAVVGVSRSRPG
                     FLVRAGAQITDMGGDLVSGPSRISMVCNVSDTNGISCNELAPGKGVARFMPTGVCLEM
                     SLGLCTRYLTIGGTSDQNRFAEIYYSSNDSVKVLTGSGQVPAMLSGATAFVAGDRVWT
                     VGGTSDSSGTNPAVPYSLQVDEAAGTVIVSEVAMGSGDVAALSRSLHRTTVLADDRSV
                     LVTGGVDRNGSIVQDAVLLRVDEAGLKVVESGLKMDKPRIGHSATLVTGGLLDGSVLI
                     TGGFSSVGGDFAAGAELYLPR"
BASE COUNT          793 a         1106 c         1019 g          506 t
ORIGIN      
        1 cgggcgaccc gcctggcccg gaggcaacag gagcggccgc gacccacaac ggaaaggccg
       61 gttggaaaag gtcaatgacg gattcatcga ccctgccctc gcccggttca aggaaaacgg
      121 gcgccagcgg catgaacgtc cgaacggccc gggccagtcc gtctgacgcg gtctcgccaa
      181 ccgattcaaa aggccgggca tcaacggcgt caagaatact ggcccgcccg cccgccggga
      241 acgccagacg aagcggattc aggcgccgcg tcacgaaagc gtgatcggac agatgctgtt
      301 tgaccaggat cgccagatcg ggccgtgaat cctcaaccgc ctggcgacca agaaggatcg
      361 aggaaaggtc atccgatgcc gtcatcacga tggatcccca cggataatcg cgaacgacgt
      421 gatcggtgat ctcccttgcg ccatgcaagc cggacatatc gcgatcggcc ggcgccgaaa
      481 tccactggaa tacgacaaga agcgcggcaa ggccacctgt tgccagccat cctgccgtac
      541 cggcgcgccc gatcatcgcg aaggcaatct cggacgcccc gattcccgcc agaacggccg
      601 tggcccaggt cgcggtcatc agaacctgaa ggtcgaaaat ccccatcggg tttataaaga
      661 cggcaaagga aaagtcggcc aggatcagaa acgaaagaat tacgacggca agcggacgcc
      721 ggcggccccc aaggacaacc cccgaaatcg cgagcggcag gatcggccac agcgactcga
      781 ccaggttgcg ccctgccatc gtggcgttgg cgacaatctc gacgatgccc gagccgccaa
      841 tccgtccgcc gaacgaatcc cggatccgct ggcctgaaag gtgggcgacg aacctgtcaa
      901 gactggatgg atcgccccag ttgagcagcg gatcgcgccc ggcggcgacc ggaaggtaca
      961 ggacgacgaa agtgcctata aacccggcca tcatgaccag aaaaccggtt cgaaacaggc
     1021 ccctgagcct gtcgggaccg caggcgccgc atcgggcaag cccgaccagg caggcaagaa
     1081 tcacaaacga accgtaaagc ggcatcgtga tgtgaacgcc ggcaccgata ccagctatgg
     1141 aagcggctgc agcaagcagc ctggcatccc tgcgctgaac cgattccaat ccaagaaaga
     1201 cgaccgcggc cagtcccagc atcgaaatgg aatagacctc ggtggtcgtg gcgtgaagcc
     1261 aggcgttcgc cgatccaagg aaggccaacg gcgcgaccca ccaaagggcg gcgggaatcg
     1321 attcagacct tcgggttacg cgatcggcaa gcagccagga catggcggcg gcaagtgcag
     1381 cggcaagtcc agacagaagg ttgatgcgga agaaggcggt tcccgcggga atgaacgaaa
     1441 acagcttgcc cgccatgcag aacgcgggga atccggtcgg atgcggaaca ccgaggccga
     1501 cggcggcgcc ggcaagttcc ccgccatccc tgaaatagac gtcgggggcg caaagggccc
     1561 acagcaccgc agttgccgca acggtaacga taacgaagcg atcgtggccc ttgacgacag
     1621 gcaccgattc acccacggcg cggcgcgaca aagcccaacc cgcaaccagg accgctgcac
     1681 cgcccgcgac cgtcaatcca gcgacaatag ccgcgaccat aagccactga atcggaaaaa
     1741 tgatggcaag tccggttacc gtggccgcga cggccgcgat tggaaccatt acgattggtg
     1801 gaatggttct tttcaaatga tcttccgaag ctgcctagcg cggcaggtac aactcggcgc
     1861 cggcggcgaa gtcaccgccg accgaactga agccgccggt gatcaggacg gacccgtcaa
     1921 gcaggccgcc ggtgaccagc gtcgcggaat gcccgatcct gggcttatcc atcttcagac
     1981 ccgactccac aaccttcagg ccggcttcat cgacacgaag gagtacggca tcctgaacga
     2041 tagacccgtt tcgatccacg ccgccggtga ccaggacgga tctgtcatcc gccagaaccg
     2101 tcgtccggtg aagcgatctc gacagcgcgg cgacgtcacc ggaacccatt gccacttccg
     2161 agacgattac agtgccggcc gcttcgtcca cctgaaggct gtatggaacg gcagggttgg
     2221 tgcctgaact gtcggatgtg ccgccgactg tccacaccct gtcaccggca acaaacgcgg
     2281 tcgctccgga aagcatcgcg ggaacctggc cggaacctgt aagcaccttg accgagtcgt
     2341 tcgatgaata atagatttcc gcgaacctgt tttgatcgct tgtaccgccg atggtcagat
     2401 acctggtgca aaggccaagc gacatctcca ggcagactcc ggtcggcatg aacctggcga
     2461 cacccttgcc gggtgccagt tcattgcacg agatcccatt ggtgtcggag acgttgcaga
     2521 ccatcgaaat gcgggacggt cccgaaacca gatcgccgcc catgtccgtt atctgagccc
     2581 cggcgcgaac caggaaacca ggccttgacc tggatacgcc cacaaccgcg gacgcgataa
     2641 tcggtgtctc catcgtggcg cgggaaaccg cgccggtcgt tgtgtcaatc aagtacatgt
     2701 acggggaata ctcgaccgga cacgaatcga cacattcctg cgactcgatc accggggatg
     2761 ccacaacgcc ctcgatggcg cccgttccgg cagttgacgt gccgccgacc acgaccaacc
     2821 tggtcgcgtc aatcatggcg gcggaggcaa gtccaaggcc ggcatcaagc gcccggtcgt
     2881 gctcgaggat ttcgaacagt ccggttcccc catccatgaa gaaggtctcg ggtgccagaa
     2941 cctcgtcggt atagccggca accttcatcc gaccggcatc caggaagctg aatccgccga
     3001 cagtcacgac gtcgcccccg gaagtcgaca cggatgcggc aaaagccctg tcaccacgcg
     3061 tattcaaggg gaaaagggat tccagtctgc acgtcccgtc caggcacagc gcgccgggat
     3121 ggacgttgta ctgcatcacc ttcgtgccat ccggacagtc gccctggtct atctcccagt
     3181 cctcgcattc aaccgtgacc gggcacgggc acacctcggg cttgtccggg tcttcacagg
     3241 gaatcacacg ccggcagtcg gcatcggtca ggcaggtcac aaccggatcg cccggttccg
     3301 gattcaacct gcgatccggc aaggcaagcc gattgaaaag gccgattgaa ttgaccggga
     3361 tgtaatagaa ggcatcaccg gtgccctgct tcgtaaccct catgccgccg cgaacgccct
     3421 tgct
//
LOCUS       DAOPPA010000363         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30090_length_3414_cov_4.385531, whole genome shotgun sequence.
ACCESSION   DAOPPA010000363 DAOPPA010000000
VERSION     DAOPPA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30090_length_3414_cov_4.385531"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKH54_10575"
     CDS             <1..560
                     /locus_tag="PKH54_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00381.1"
                     /translation="DVRKSDDLLQAMNKRLAEVATDWIVFDGSKAGWNVSIIRSEFKT
                     GMGEEALHVQEGYEHFYYFFTNGVLYKLVRTWESKDFGPVRQALAAAYGNPLYQNRPG
                     DADRRFISELRWHGSNITVDVEDWTESFNSYTVRWADVAVDAAVMKSWGESGSDLPRL
                     NPLIDAAKEQPKKNAKDPVDDLLGR"
     gene            605..1258
                     /locus_tag="PKH54_10580"
     CDS             605..1258
                     /locus_tag="PKH54_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD00382.1"
                     /translation="MAGVKIHRIVVGPFDTNCILVEDEASKEALVVDPGGGVDLISER
                     VRLTGVRPVMVVLTHGHVDHCAEASAIATMYGIPIAMHRDDLLLYQHMDVQVEAFMGP
                     AAATRYRGAQQVMPTVFLEHGQLLKVGRTNARVIHLPGHTMGGIGLLFEGRPDIIVQG
                     DTLFRDGVGRTDLYGGNWEALQSSLKLRLFTLKPDTIVVCGHGGDTTISREKVDISW"
     gene            1278..1652
                     /locus_tag="PKH54_10585"
     CDS             1278..1652
                     /locus_tag="PKH54_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOD00383.1"
                     /translation="MVDFPVTPARKQALLQRMADLGIVEADLDEQFTHSRGNGGQNVN
                     KVSTCVILTHRPTGISVRCEEERTQGLNRYRARQRLADLIDYEVNGVKSRAGVEAEKA
                     RKRKADRRRRSVAKAESHRAET"
     gene            complement(1634..2518)
                     /locus_tag="PKH54_10590"
     CDS             complement(1634..2518)
                     /locus_tag="PKH54_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00384.1"
                     /translation="MPPLTRASLDRWMFNKDGMDYGPFSVREIEQMIDSGELLGTSEI
                     INYKSKIVAPVSQVPYFAQYLDEKEKRDREAARKAEIEHDHTTAIKDSKRRHTLPLAI
                     GGVLVVVFSITAWALLRDPEIPTSGYPVTFFRDLTFPQLNPLKPRLASPVEFVAEDED
                     KAESAAKVARKPRGPSRQAPGVVPEPVLDFSFSGSAEATREFSSGDLEVLKSGVTPGL
                     IRCFQKELSSVEGFSGGKIVFYVMPKGKVALSKVETKPAASGSLVSCVSSAVNSKKIP
                     PYKGDIQIIEIPLYVSAR"
     gene            complement(2823..>3414)
                     /gene="lipB"
                     /locus_tag="PKH54_10595"
     CDS             complement(2823..>3414)
                     /gene="lipB"
                     /locus_tag="PKH54_10595"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOD00385.1"
                     /translation="LQADFAGEVRAGGSHGVIIAIRHPAVITMGRRASESELHVTPER
                     LAELGIDLFNVDRGGGATFHYPEQAVLYPILNLTALKLTVPALLKISGDAVLEALKSH
                     GVQGFWDESRPGVYMPDGAKIASVGYHLSKGLTTHGIAINTGRGWDGFKLIDPCKVAN
                     QKITSVQDLTGVAPDPDQFALAVASSMKRMLGAGRT"
BASE COUNT          691 a          956 c         1049 g          718 t
ORIGIN      
        1 aggatgtgcg caaaagtgac gatctgcttc aggcaatgaa caagcggctt gccgaagtcg
       61 cgaccgactg gatcgtgttt gacggctcca aggccggctg gaacgtgtcg ataatccgca
      121 gcgagttcaa gaccgggatg ggcgaggaag ccttgcatgt ccaggaaggc tacgaacatt
      181 tctattactt cttcaccaac ggtgttcttt acaaactggt gcggacgtgg gaatccaagg
      241 atttcggccc ggtgcgccag gcgcttgccg ccgcttacgg caatccgctt taccagaaca
      301 ggccgggcga tgccgacagg cgcttcattt ccgagctgcg ctggcacggc tcaaacataa
      361 cagtcgatgt cgaggactgg actgaatcgt tcaactctta tacagtccgc tgggctgacg
      421 tcgcggtgga tgcggcggtg atgaagtcct ggggcgaatc cggaagcgac ctgcccaggc
      481 tgaatccgct gattgacgcc gcgaaggaac agccgaagaa aaatgccaag gatccagttg
      541 acgaccttct gggtcgttga taatgttccc tgcatagtta tagtgacaag ctgagtaatg
      601 tgatatggca ggcgtaaaaa tacatcgtat tgttgtcggt cccttcgaca cgaactgcat
      661 ccttgtcgag gatgaggcgt cgaaggaggc cctggtggtt gatccgggcg gcggagtcga
      721 tctgatttcg gaacgggtca ggcttaccgg ggttcgtccg gtcatggtcg tgctgaccca
      781 cggtcacgtg gatcactgtg ccgaggcttc ggcgattgcg accatgtacg ggataccgat
      841 tgcgatgcat cgggacgatc tgctgcttta tcagcacatg gacgtccagg tcgaggcctt
      901 catgggtccg gccgcggcca cccgctatcg cggcgcgcag caggtcatgc cgacggtttt
      961 tcttgaacac ggacaacttc tcaaggtcgg gcgcaccaat gcccgggtca tccatctgcc
     1021 tggccacacg atgggcggaa tcggcctgct gttcgaaggc aggccggaca tcatcgtcca
     1081 gggggacacc cttttccggg acggcgttgg aagaaccgac ctttacggcg gaaactggga
     1141 agcccttcag tcgtccctga aattgcgcct tttcacactg aagcccgaca cgattgttgt
     1201 ctgcgggcat ggcggcgata ccacgatcag ccgcgaaaag gtcgatattt cctggtaatt
     1261 cactggccgg ttgccggatg gttgattttc ccgtcactcc agctcgaaaa caggccctgc
     1321 tgcaacggat ggccgatctt ggcatcgtcg aggccgacct tgatgaacag ttcacgcatt
     1381 cacgtggaaa cgggggacag aacgtcaaca aggtttccac gtgcgtgatc ctgacgcacc
     1441 gtccgacagg catttccgtg cggtgcgagg aagaaagaac ccagggcctg aaccgttacc
     1501 gcgcccgtca gcggcttgcc gacctgatcg attacgaagt caatggcgtg aagtcccggg
     1561 ctggtgtcga ggcggaaaag gccagaaaga gaaaggccga tcggcgcagg cgttcggtcg
     1621 cgaaggctga aagtcaccgc gcggaaacgt aaagcggaat ttcgatgatc tggatatcgc
     1681 ccttgtaagg cgggatcttt tttgaattga cggcagaaga tacgcaagat acaaggcttc
     1741 ccgacgcggc tggcttcgtt tcaaccttcg aaagcgcgac ctttcccttc ggcatcacat
     1801 aaaagacaat ctttccgccc gagaatccct cgacgctcga aagttccttc tggaagcatc
     1861 gaatcaggcc gggcgtgacc cccgacttca gcacttccag gtcgccggat gaaaattcgc
     1921 gggtcgcctc ggccgatccg gagaacgaga agtcgaggac gggctccggt accacgccgg
     1981 gggcttgccg ggaagggccg cgaggtttgc gggcgacctt ggcggcgctt tcagccttgt
     2041 cttcgtcctc ggcgacgaat tcgaccgggg atgcaagccg tggcttcaac gggttcaact
     2101 gcgggaaagt aaggtcacgg aagaacgtga ccggatagcc ggaagtcggg atttcgggat
     2161 cgcgaagcag tgcccaggcc gtgatggaaa acacgacgac aaggacgccg ccgattgcca
     2221 gcggaagcgt gtgccgccgt ttgctgtcct tgattgcggt ggtgtggtcg tgctcgattt
     2281 cggcctttct ggccgcctcg cggtcacgtt tttccttttc gtccagatac tgggcgaaat
     2341 acgggacctg ggaaaccggc gcgacgatct ttgacttgta gttgatgatt tccgaggtgc
     2401 cgagcaactc gccggaatcg atcatctgtt caatttcgcg gacggaaaac gggccgtagt
     2461 ccatcccgtc cttgttgaac atccatcgat ccaggcttgc tcgtgtcagc ggcggcatga
     2521 ttacccgtca agacccccct tgaccagccg gcgaaccggt cgggtcatcg ggttatgtat
     2581 caggaaaaga aaccccggac aagggtcagt cgtgtaaggg tgaattatta ttcgaccgtg
     2641 cgcggatgtg cggattgttg ccggtcgatg atggcgggcg aattttcatt cgcccttaca
     2701 aggctgggat tttcgttcgc gcgcccagcg ggcgaatttt cattcgccct tacaaggccg
     2761 ggattttcat tcgcccttac aaggccggga ttttcattcg cgcgcccagc gggatttgtg
     2821 gactacgtcc ggcccgcgcc cagcatcctt ttcatggaac tggcgacggc aagcgcgaac
     2881 tggtcggggt cgggcgcgac tccggtcaga tcctggacac tggtgatttt ctggttcgcc
     2941 accttgcacg ggtcgatcag cttgaatccg tcccatcccc ggccggtatt gatcgcgatt
     3001 ccgtgcgtgg tcaggccctt tgaaaggtga taaccgactg acgcaatttt tgccccatcg
     3061 ggcatgtaaa ccccgggccg cgattcgtcc cagaaaccct gcacgccgtg cgatttgagc
     3121 gcttccagca cggcgtcgcc cgatatcttc agaagcgccg gcaccgtcag cttcagggcc
     3181 gtcaggttca gtatcggata aaggacggcc tgctcgggat aatggaatgt cgcgccgccg
     3241 ccccgatcga cgttgaaaag gtcgataccg agttccgcca gccgttccgg cgtcacgtgc
     3301 agttcggatt cgctggcgcg acgacccatg gtaatgaccg ccgggtggcg gattgcaata
     3361 atgacgccgt ggctgccgcc cgcccggact tccccggcga agtcggcctg aagt
//
LOCUS       DAOPPA010000364         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30129_length_3410_cov_3.728763, whole genome shotgun sequence.
ACCESSION   DAOPPA010000364 DAOPPA010000000
VERSION     DAOPPA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30129_length_3410_cov_3.728763"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PKH54_10600"
     CDS             complement(<1..1059)
                     /locus_tag="PKH54_10600"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00386.1"
                     /translation="MKVRSVIESACKSPSGFRLPAGAKAAWAGLVAPCILALATVLCG
                     GCAGDGGSSDVINDTGTDAISDTVTDQGAWCDPEPLYAAVDPSIGTGGGGFWGGGNGF
                     MGAAAPFGLVRPGPDTQGDGDTFDPTHHSGYWYHDPYIAGFSQTHMHGTGITAYGNFM
                     ISPVFDLDAPLMYESEYRQAFDKATELARPGHYEVTLESGVTAELTATPRVAYHRYHF
                     PDDGREPCLMLNMGHALTMNFIIESALDLDATTSAFSGHLKQLGGFSFEIGGLTVWIN
                     GRLVQPPSRVMSWDDNFGFRDGNAGCVGAMSCVVAACWPAGTPEVEVRVAVSFVSAEG
                     AAANLDAEGQETFDRTTEN"
     gene            1275..1688
                     /locus_tag="PKH54_10605"
     CDS             1275..1688
                     /locus_tag="PKH54_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD00387.1"
                     /translation="MAEHVNPIPVAACILVHRDRVLLLKRTIPPCEGRWSLPSGHYEI
                     GESAEEGMIREILEETGLSVTVRYIGSGARVLPNGVPYLSLLFWAEAPSDSITLDCEN
                     SDWAWVPMVPDKLKVFDWAFSNQLQAVLDFIDRCD"
     gene            complement(1811..2671)
                     /locus_tag="PKH54_10610"
     CDS             complement(1811..2671)
                     /locus_tag="PKH54_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOD00388.1"
                     /translation="MGYVPVTKLEGVSVFRKAAMGTWQTCKDPQVYSIMEVDVTDIIP
                     MMEEYGKEHGAKITFSHVVGKAITYCLLRRPEINGFLRGSRIWLRKDVRLNYLVNIPG
                     EGAEKVKKATLSTTAIPDCETRTIAGIAQELSARAEVVRQQKDPEIKKNMDMWKVIPW
                     WMMKYYLSFASWLIYGLNINIPGIPKDPFGSVMITNIGTMGIGPTWAPLVPYSRVPLL
                     ITLCSAAKKAWVVNDEIKIRTILPVCITYDHRFMDGAQASQMFADFHECFRKPQELLF
                     AGPATENVKK"
     gene            3034..3366
                     /locus_tag="PKH54_10615"
     CDS             3034..3366
                     /locus_tag="PKH54_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOD00389.1"
                     /translation="MIDDSVPLVPRLRRSVLVTRVRKAARRREYLMLPHARQWSLERS
                     VRAPDIEHALLHGHPVPSRDRFVKERGRWSYCFEGKDVDGRELRVIVAFQGLMLVVTV
                     VRLAELEN"
BASE COUNT          653 a          992 c         1037 g          728 t
ORIGIN      
        1 gttttcggtg gttcggtcaa aagtttcctg cccttccgca tcaagattcg cggcggcgcc
       61 ttcggcggac acgaacgaaa ccgcgacccg aacctcgact tccggcgtgc ccgcgggcca
      121 gcatgccgcg accacgcagg acatcgcgcc gacgcagccg gcattgccgt ccctgaatcc
      181 aaagttgtcg tcccacgaca tgacgcggga tggaggctgg accaggcggc cattgatcca
      241 aacggtaagg cccccgattt caaaggaaaa tccaccaagt tgcttaagat ggccggaaaa
      301 cgcgctggtc gttgcgtcca gatccagggc ggattcgatg atgaagttca tcgtcagggc
      361 gtgccccatg ttcagcatca ggcacggctc gcgtccgtcg tccgggaaat ggtaacggtg
      421 gtatgcgacg cgcggcgtgg cggtcagttc ggcggtgacc ccggattcaa gggtcacctc
      481 ataatggccg ggacgcgcga gttcggtcgc cttgtcgaag gcctgccggt attcggattc
      541 gtacatcagc ggggcgtcaa ggtcgaaaac cggggaaatc atgaagttcc cataggcggt
      601 tatgccagta ccgtgcatat gcgtctgcga gaagccggct atgtacgggt cgtggtacca
      661 gtatccggaa tggtgcgtcg ggtcgaaggt gtcgccgtcg ccctgggtgt ccggccctgg
      721 acggaccagc ccgaaaggcg cggccgcccc catgaagccg tttccgccgc cccagaaacc
      781 acccccgccc gtgccgatcg aaggatcgac cgcggcgtaa agcggttcgg ggtcgcacca
      841 ggctccctga tcggtgaccg tgtcgctgat ggcgtcggtg ccggtgtcgt tgatgacgtc
      901 ggaactgccg ccgtccccgg cgcacccgcc gcaaaggact gtggccagag ccaggatgca
      961 gggcgccacc aacccggccc aagcggcctt tgccccagcc ggcaggcgga agccggacgg
     1021 cgatttgcaa gccgattcga tgactgacct tactttcatg aagaaccccg catgagtgcg
     1081 ccggaaccga tcccgcctgc gcgggaattg tcagcaactg tcagtttaag tcgccctgtc
     1141 gaatgatgca atactgcaag atgttgaacg gtttgcgacc aggtggtaaa agcgagctgg
     1201 gccgggccag cgggatccgg ccggatctgc cggatttggt cgtgcggtct gttccagaca
     1261 agggggaaga cgccttggcc gaacatgtga acccgattcc cgtcgccgcc tgtatccttg
     1321 tccatcgtga tcgcgttctg cttctgaagc ggaccattcc tccatgcgag gggcggtgga
     1381 gccttccttc cggccactac gagattggcg aaagcgcaga agagggcatg atccgagaaa
     1441 tccttgaaga aacgggtctt tcggtcaccg taaggtacat cggttccggt gccagggtgc
     1501 ttccgaacgg tgttccgtac ctttccctgc ttttctgggc cgaggcccct tccgacagca
     1561 tcaccctgga ttgcgaaaac tccgactggg cctgggttcc gatggttccc gacaagctga
     1621 aggtattcga ctgggccttt tcgaaccagc ttcaggccgt cctggatttc atcgaccggt
     1681 gcgattgaat cgcgggttga tatcgcgacg cgccgtgaca atccctgccc ctgtcgggat
     1741 gattccggga cgtgtctgga tgattcgtat ccgtgccggg agtgtgggcc gatctggcca
     1801 gcccgatcgc ctacttcttg acgttttcgg tcgccggtcc ggcaaaaagc agttcctggg
     1861 gcttgcggaa acattcatgg aagtccgcga acatctggga tgcctgggcg ccgtccatga
     1921 acctgtggtc gtaagtgatg cagacgggca gtatcgtgcg aatcttgatt tcgtcgttca
     1981 cgacccatgc cttcttggcc gccgagcaca gcgttatcag aagcggaaca cgtgaatatg
     2041 gaaccagtgg cgcccacgtc ggcccgatgc ccatcgtgcc gatgttggta atcatcacgg
     2101 agccgaaggg atccttcggg attccgggga tgttgatgtt caggccgtaa atcagccatg
     2161 acgcgaagga caggtaatac ttcatcatcc accacgggat gaccttccac atgtccatgt
     2221 tcttcttgat ttccgggtcc ttctgctggc gtacgacttc ggcccttgcc gaaagctcct
     2281 gggcgatgcc ggcaatcgtc cttgtttcgc agtcgggaat cgcggtggtc gaaagcgtcg
     2341 ccttcttcac cttttcggcg ccttcccccg gaatgttgac cagatagttc agccggacat
     2401 ccttgcgaag ccagatccgg ctgccgcgca gaaagccgtt gatttccggg cgccgaagca
     2461 ggcaataggt gattgccttg ccgacgacgt gcgagaacgt gatcttcgcg ccgtgttcct
     2521 tgccgtattc ctccatcatc gggatgatgt cggtgacatc gacttccatg atggaataaa
     2581 cctggggatc cttgcatgtc tgccatgtac ccatggccgc cttgcggaaa acggaaacac
     2641 cttcaagctt cgtgactgga acgtaaccca tgattccccc gcgggtgact tgtgtcggat
     2701 gtagtaaatc acaactgccg tttttgaaaa ctgaaaatta acgcgttgcg acagaaattt
     2761 tttcgcaacg cggcattaga gggtcagtca ccggcgtttc atgacgaaac cgggcaggtt
     2821 cgggcgagcc cgggcgcctt tcgacaatcc cgggcgcctt tcgacaatcc cgggcgcttt
     2881 cccacaaacc ggggcaccct tcgtccaatt cgatccttcc gttgccctgg cccttccaga
     2941 cgaaacaaac gaaccccggt ttcgaaaaag gaatccgggc cacgccttgt ttcaatccat
     3001 gttagttgct aacattccat ggagcgatgc ctgatgatcg acgattctgt gcccctggtt
     3061 ccaaggctca ggcgctcggt acttgtgacg cgcgtcagga aggcggcccg gcgtcgtgaa
     3121 tacctgatgc tgccgcacgc caggcagtgg agcctggaaa ggtcggtccg ggccccggat
     3181 attgagcatg cgcttcttca cgggcatccg gtgccttcaa gagaccgctt cgtcaaggag
     3241 cgcgggcgct ggagctactg cttcgagggg aaggatgtcg atggtcgcga actgcgcgtt
     3301 atcgttgcgt ttcagggact gatgcttgtg gtcacggtgg taaggctggc ggaactggag
     3361 aactgactca tggctgatgt gattttcatg gatcttggtt ttccggtgac
//
LOCUS       DAOPPA010000365         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30542_length_3376_cov_4.579042, whole genome shotgun sequence.
ACCESSION   DAOPPA010000365 DAOPPA010000000
VERSION     DAOPPA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30542_length_3376_cov_4.579042"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3360
                     /locus_tag="PKH54_10620"
     CDS             <1..3360
                     /locus_tag="PKH54_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD00390.1"
                     /translation="RCCSCMMTVDGSPNVMTCVTPLKAGMIVESPDRERDAGYFKRMP
                     GRKPDSTVAPEGDNRPGLKLDLAVIGAGPAGLEAALMAHEAGVEKIALFDDKEYAGGQ
                     LKLQTHDFFGTQALGASVRGFEIGNQLEKRVRESCIDLRLNSTVVGMFPENWLIFRDG
                     DRINHAHAKKIVVATGASEKMIPFPGNYLPGVIGAGGAQTFMNIHGVRPGRRVLIIGA
                     GNIGVILAYQLLQAGIEVVEVVEAAGHIGAYGVHASKLRALGVPINTRHTIKAAYGTD
                     HVEAADIVRLDENWREIPDSVRRVECDTICLAVGLSPLNELLWQAGCEFQWVPELGEI
                     PKMDRYRRTSHRDIFCAGDCAAIGEASIARLEGRIAGLVAARDLGHEHPRFVAALEEA
                     FRLLGSIQSGKFGEKLGVGKAKACGEPIPEPLKIRPFRQASDLSGVTEITRFKVILDC
                     PQDIPCNPCEVSCGFGAIIVGSEINQPPDVDLEKCTGCGACVGKCPGRAIRLVKLDAG
                     EGPEGPVSEVRVPWEFQPTPDIGSTVTVLDADGAPIGTGVVTRILGTRSKQDCTILGL
                     KVAREIAMKVRTVPPPVQALPESDVTDAAQPVDELICRCEEVSRRSLENCIAAGYTSI
                     NDLKRLLRTGMGPCRGSGCRSVLEGRLAQVESVDKAALVAAKSARKTVSRPPVKRVTI
                     GEAARMSFSQADLQELDELNRRNTVLLPELDKLAWTDIGISRRMKKKAVIIGGGISGV
                     LTAWHLARMGWTDIAVVEKEFLAAGKTGACLGGLRTGFASANKVARARMGLEIMRDAE
                     KLLGDSIGWFQGGYVYLAYDDGQADSFRKAAAVWDEGRVPYRYLTTESDLAHYVPGID
                     ASKVKLGVFFEEAGGANPFLATYRFADDARKMGAEFIVGREVADIRVQDNRVRGVVLN
                     DGTLIECEHVVNCAGAFAFRVSGMIGLDLTDRFWIERHGSMITEKLPFWMDALVVSYH
                     PTLSGYWQQKRMEKDAIEGEIVACYSPDRPWHGYNTKSHVEFMSRMARSMLEVQPGLA
                     EVGLVRHAAHHYVGRVSGIPLIGPCGEFDGRRLPGPTGFWHNIAKKGHGFMCAPGDGY
                     ALAWSMVNGELHPWISECTLEEDPSTIETMK"
BASE COUNT          649 a         1041 c         1112 g          574 t
ORIGIN      
        1 cgctgctgca gttgcatgat gaccgttgac ggcagcccga acgtgatgac ctgcgtgacg
       61 ccgttgaagg cgggcatgat tgtcgaatcg ccggaccgtg aacgcgacgc cggctatttc
      121 aagcgcatgc ccggacgcaa gccggactcc acggtggccc cggaaggcga caatcgcccc
      181 ggcctgaagc tggacctggc cgtgatcggc gccgggccgg ccggccttga agctgccctg
      241 atggcccacg aggcgggcgt cgaaaagatc gccctgttcg acgacaagga atacgccggc
      301 ggacagttga aactccagac acacgatttc ttcggaaccc aggcgcttgg tgcctcggtt
      361 cgcggcttcg agatcggcaa ccagcttgaa aaacgcgtcc gggagtcttg catcgacctg
      421 cgcctgaact cgaccgtggt cggcatgttc ccggaaaact ggctgatttt ccgtgacggc
      481 gaccgcatca accatgcaca cgcgaaaaag atcgtcgtcg cgaccggcgc ttccgaaaag
      541 atgatcccgt tcccgggaaa ctacctgccc ggcgtcatcg gcgcgggcgg cgcgcagacg
      601 ttcatgaaca tccacggcgt gcggcccggg cggcgcgtgc tgatcatcgg ggccggaaac
      661 atcggcgtta tcctggcata ccagcttctt caggccggca tcgaggtcgt cgaagtcgtc
      721 gaggcggcag gccacatcgg cgcttacggc gttcacgcca gcaagttgcg cgcccttggc
      781 gtcccgatca acacccggca caccatcaag gcggcctacg ggaccgacca tgtcgaggcc
      841 gccgatatcg tgcgtcttga cgaaaactgg cgcgaaatcc ccgacagcgt ccgacgtgtc
      901 gaatgcgaca cgatctgcct ggcggtcggc ctttcaccgc ttaatgaact gctgtggcag
      961 gccggttgcg aattccagtg ggttccggaa ctcggcgaaa tcccgaagat ggatcgttac
     1021 cgccggactt cacaccggga catcttctgc gccggcgact gcgccgcaat cggcgaggca
     1081 agcatcgcca ggctggaagg caggatcgcc ggcctggtcg cggccaggga cctgggccat
     1141 gaacacccgc gattcgtcgc cgcgcttgag gaagctttcc gcctgcttgg gtcgatccag
     1201 tccggaaaat tcggcgaaaa acttggcgtc ggcaaggcga aggcctgtgg cgagccaatc
     1261 cccgagcccc tgaagatcag gcctttcagg caggcctccg acctgtcggg cgtcacggaa
     1321 atcaccagat tcaaggtcat cctcgactgt ccccaggaca tcccgtgcaa tccctgcgag
     1381 gtaagctgcg gcttcggcgc catcatcgtc ggttcggaaa taaatcagcc gcccgacgtc
     1441 gatctggaaa aatgcaccgg ttgcggggcg tgcgtcggca agtgcccggg acgcgccatc
     1501 aggctggtca agcttgacgc cggcgaaggc ccggaagggc cggtttcgga agtccgcgtg
     1561 ccctgggaat tccagccgac accggacatc ggctcgaccg tgaccgtgct ggatgcggac
     1621 ggtgccccga tcggcacggg cgtcgttaca aggatccttg gaacaaggtc aaagcaggac
     1681 tgcaccatcc ttgggctgaa ggtggcacgc gaaatcgcga tgaaggttcg cacggttccc
     1741 ccgccggtcc aggccctgcc ggaatccgac gtgaccgatg ccgcgcagcc cgttgacgaa
     1801 ctgatctgcc gctgcgagga agtgtcgcgg cggtccctgg aaaactgcat tgccgccgga
     1861 tatacgtcga tcaacgacct gaagcgcctg ctgcggaccg gcatgggtcc ctgccgcggt
     1921 tccggctgcc gttcggtgct cgagggcagg cttgcccagg tcgaaagcgt tgacaaggcg
     1981 gccctggtcg cggccaagtc ggccaggaag accgtttcca ggccgccggt caagcgcgtg
     2041 acgatcggcg aggcggcccg catgagcttt tcccaggccg acctgcagga acttgatgaa
     2101 ctgaaccggc gcaacacggt tcttctgccc gaactggaca agctggcctg gaccgacatc
     2161 gggatttcgc ggcgcatgaa gaagaaggcc gtcatcatcg gcggcggcat cagcggcgtc
     2221 ctgacggcgt ggcacctggc caggatggga tggaccgata tcgcggtcgt cgaaaaggaa
     2281 ttcctggcgg ccggcaagac cggtgcgtgc ctgggcggcc tgcggaccgg tttcgcatcg
     2341 gcgaacaagg tcgcgcgcgc ccggatgggc ctggaaatca tgcgcgacgc cgaaaagctg
     2401 cttggcgatt cgatcgggtg gttccagggc ggctacgtct atctggcgta tgacgacggc
     2461 caggccgaca gtttcaggaa ggccgcggcg gtgtgggacg aaggccgggt tccgtaccgg
     2521 tacctgacga cggaatccga tcttgcgcac tacgtcccgg gaatcgacgc gtcgaaggtg
     2581 aagcttggcg tattcttcga agaggccggc ggggccaatc cgttcctggc aacgtaccgg
     2641 ttcgccgacg acgccaggaa gatgggcgcc gaattcatcg tcggacggga ggtcgccgac
     2701 atcagggtcc aggacaatcg ggtccgcggc gtcgttctta acgacggcac cctgatcgaa
     2761 tgcgaacacg tggtcaactg cgccggggcg ttcgcgttcc gcgtgtcggg gatgatcggc
     2821 cttgacctga ccgaccgctt ctggattgaa cgccacggct cgatgattac cgagaagctg
     2881 cctttctgga tggacgccct ggtggtttcg taccacccga cgctttccgg atactggcag
     2941 cagaaacgga tggaaaagga cgcgatcgag ggcgagatcg tcgcgtgcta ttcgccggac
     3001 aggccgtggc acggatacaa cacgaagtca cacgtcgaat tcatgtcccg gatggcgcgt
     3061 tcgatgctgg aagtccagcc cgggctggcc gaagtcggcc tggtcaggca cgccgcgcat
     3121 cactacgtcg gccgcgtttc gggcattccg ctgattgggc cgtgcggcga gttcgacggg
     3181 cggcgcctgc cgggcccgac cggtttctgg cacaacatcg ccaagaaggg ccacggattc
     3241 atgtgcgcac cgggcgacgg ttacgcactg gcctggtcga tggtaaacgg cgaactgcac
     3301 ccgtggattt ccgaatgcac ccttgaagaa gacccctcaa cgatcgaaac gatgaagtaa
     3361 gggcgaatga aaattc
//
LOCUS       DAOPPA010000366         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30680_length_3364_cov_3.116954, whole genome shotgun sequence.
ACCESSION   DAOPPA010000366 DAOPPA010000000
VERSION     DAOPPA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30680_length_3364_cov_3.116954"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1675
                     /locus_tag="PKH54_10625"
     CDS             2..1675
                     /locus_tag="PKH54_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOD00391.1"
                     /translation="MLSLGVIDWVVVALYFVAIIGIGLYLRGFTKTGEDFFMGGRKNS
                     SWVAGLAFLSANLGALEILGWTGGTMKYGMFVSHWYWLGAIPAMLFLGLYMMPFYYSS
                     KIHSVPGYLKLRYDERTRLLNAISFAVMTLLMSGINLYLMALVFNVLLGWDWHVSMWV
                     SAAAIGCYITLGGLMSAIFTEIVQFFLIWAGLFLVTILGVVELGGPAEVFANLPDKMT
                     TLWQTTGAASSNDMGVTWLGLVMGLGFVLSFGYWTTDFLVVQRAFSSKDLRSARLTPI
                     TASFFKMALPFIVVGAGLVAFQLTRRADMGFELLPHPSTGEAWYDSALPMLIARYYPH
                     GLVGLGITALLAGFMAGQAGNVSAFNTVWTYDIYKAVINRKASDAHLVWVGRVTTVVG
                     ILLSVATAYVAMQFNTIMDYIQAIFSWVNAPLFATMLLGMFWKRTTSAGAFWGLIAGM
                     LVSFGIYMGFKFNLFGPTVATILTLAEHPSDMTRNLWQATWGWIVCFGGTILVSLFTV
                     PKPDSELVGLVKGLTVEEDGSVKVAWYGRPAFWAAVSLAVLIVLNILFW"
     gene            1692..1907
                     /locus_tag="PKH54_10630"
     CDS             1692..1907
                     /locus_tag="PKH54_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00392.1"
                     /translation="MNNEGKQPLPIWFFVGVILFVYGILVIVGQAMDPGGQALKSLKP
                     GFIWGPVMIVFGVIFTAIGLVAHKRQG"
     gene            1927..2325
                     /locus_tag="PKH54_10635"
     CDS             1927..2325
                     /locus_tag="PKH54_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00393.1"
                     /translation="MRKVTTRRPSFPEVAADFPISFPEGERWNVLCGDAGHWRVGAFS
                     PPECSAAEVGELEIHDCPELFLLISGSVTLVISDMNGGVTELPLQPMKPVLVTSPHSA
                     YCPDGPFSGTCLVVERDEFDTEYRLPADWR"
     gene            2346..>3364
                     /gene="galK"
                     /locus_tag="PKH54_10640"
     CDS             2346..>3364
                     /gene="galK"
                     /locus_tag="PKH54_10640"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOD00394.1"
                     /translation="MIQSQLMDRIRESFRNEFSRVPEVWADAGGRVNIIGEHTDYHDG
                     FVLPAAIDLRTCAVAAGRDDGILRIHAVDFGGTVEIALTGLKPFERIDWRSYTCGPFW
                     AWIRSGRPFSGADVAVGGDVPSGGGLSSSASVEVSLVGIAAGLSLSAPPCPDVAAIAR
                     TAENLFCRVPCGIMDQMASACGHEGKALLLDCRTLQTTLVDVPAGWVLVVADSGVRHS
                     LAGGEYARRQKECADGMRFVVNSFPTVRAARDVSMEMLESVRQGMSEVSFRRLRHAVS
                     ENDRTVRAASALQAGDVAEVGLLMHGSHRSLAHDYEVSCAELDHLVEIASRIDGCAGA
                     RLTGAG"
BASE COUNT          545 a          975 c         1082 g          762 t
ORIGIN      
        1 catcctgagt ctgggtgtga tcgactgggt ggtggtcgcg ctttatttcg tcgcgatcat
       61 cggcatcggc ctgtaccttc gcgggttcac caaaaccggc gaggacttct tcatgggcgg
      121 gcgcaagaac tcttcctggg ttgccggcct tgccttcctg tcggccaatc tcggtgcgct
      181 ggagatcctg ggatggaccg gcggcacgat gaagtacgga atgttcgtgt cccactggta
      241 ctggcttggg gccatcccgg cgatgctgtt cctgggcctt tacatgatgc cgttttatta
      301 cagctcgaag atccattcgg tgccgggtta cctgaaactg cggtacgacg agcgcaccag
      361 gctcctgaac gccatatcgt tcgccgtcat gacgcttctg atgtccggga tcaacctgta
      421 cctgatggcg ctggttttca acgtccttct ggggtgggac tggcacgtgt cgatgtgggt
      481 gtccgcggcc gccatcggct gctacatcac gctgggcggc cttatgagcg ccattttcac
      541 cgagatcgtc cagttcttcc tgatctgggc cgggcttttc ctggttacga tccttggcgt
      601 tgtggaactt ggcggtccgg cggaagtctt cgccaacctg cccgacaaga tgaccacgct
      661 gtggcagacg accggggccg cgtccagcaa cgacatgggc gttacatggc tgggtcttgt
      721 gatgggtctg ggattcgtcc tttcgttcgg atactggacg acggacttcc tggttgtcca
      781 gcgcgccttc tcgtcaaagg acttgcggtc ggcacgcctg accccgatta ccgcctcttt
      841 cttcaagatg gcccttccat tcatcgtggt cggggccggc ctggtcgcct tccagcttac
      901 caggcgggct gacatgggat tcgagctcct gccgcatcca tcgaccggcg aggcatggta
      961 tgactcggcg ttgccgatgc tgattgccag gtattatccg cacggcctgg tcgggctggg
     1021 cattaccgcc cttctggccg ggttcatggc cggacaggcg ggaaacgtca gcgcgttcaa
     1081 cacggtctgg acctacgaca tctacaaggc cgtcatcaac cgcaaggcat ccgacgccca
     1141 cctggtatgg gtcggccgcg tcacgacggt ggtcggaatc ctgctgtcgg tcgcgacggc
     1201 ttacgtggcc atgcagttca acacgatcat ggattacatc caggcaatct tctcgtgggt
     1261 caacgcgccg cttttcgcga ccatgctgct tggcatgttc tggaagcgca ccaccagcgc
     1321 cggggcgttc tggggcctta tcgccggcat gctggtttcc ttcggcatct acatgggttt
     1381 caagttcaat ctgttcgggc cgacggtcgc gacgatcctg acgctggcgg aacaccccag
     1441 cgacatgacc aggaatctgt ggcaggccac gtggggatgg atcgtctgct tcggcggtac
     1501 catccttgtc agccttttca ccgtgccgaa gcccgattcg gagcttgtgg gccttgtgaa
     1561 gggcctgacc gtcgaggaag acggatccgt gaaggtggca tggtacggca ggccggcctt
     1621 ctgggccgcg gtttcgctgg ctgtcctgat agtccttaac atcctgttct ggtgaaccgg
     1681 aggtatctga aatgaacaac gaaggaaagc agccgctccc gatttggttc tttgtcggcg
     1741 tcatcctttt cgtctatggc atcctcgtga tcgtggggca ggcgatggat cccggcggac
     1801 aggcgttgaa gtcgttgaag cccgggttca tctggggccc ggtcatgatt gttttcggcg
     1861 tcatcttcac cgcaatcgga ctggtcgcgc acaaacggca gggctgacgg ccccggggtg
     1921 gtcttcatga gaaaggttac aaccaggcgg ccttcgtttc ccgaggtcgc ggccgatttt
     1981 cccatcagct ttccggaagg tgagcgctgg aacgtgcttt gcggtgatgc gggccattgg
     2041 cgggtcggtg cgttcagtcc accggaatgt tcggcggccg aggtcggcga actggagatt
     2101 cacgattgtc cggaactgtt cctgctgatt tcgggttccg tgacacttgt gatttccgac
     2161 atgaacggtg gcgtcaccga actgccgttg cagccgatga agcctgtcct ggtcacttcc
     2221 ccgcactcgg cgtattgtcc cgacggtccg ttttccggca catgccttgt tgtcgagcgg
     2281 gacgaattcg ataccgaata ccgcctgccc gccgactggc gctgaactga aacaagaggc
     2341 acaagatgat tcaatcccaa ttgatggaca ggatccgcga aagcttcagg aacgagtttt
     2401 ccagggttcc cgaggtctgg gccgacgctg gcggccgcgt caatatcatc ggcgaacaca
     2461 ccgactatca tgacggcttc gtgctgccgg cggcgattga tcttcgtacg tgtgccgtgg
     2521 cggccggtcg tgacgatggc atcctgcgca ttcatgcggt cgatttcggc ggtaccgtcg
     2581 agatcgccct tactggcctg aagccgtttg agcggatcga ctggcggtca tacacgtgcg
     2641 ggccgttctg ggcctggatt cgttccggca ggccgttttc cggcgccgac gtggcggtcg
     2701 gcggggatgt gccgtccggc ggcggacttt cctcgtcggc ctccgtcgag gtttcactgg
     2761 tcgggattgc ggccgggctg tcgctttccg ccccaccctg ccccgatgtc gcggcgatcg
     2821 caaggacggc cgagaacctt ttttgccgtg taccgtgcgg gataatggat cagatggcga
     2881 gcgcctgcgg acatgaggga aaggcgctgc ttctggactg ccggactctt cagacaacgc
     2941 ttgtcgatgt tccggccgga tgggttctgg ttgttgccga ttccggcgtc aggcattcac
     3001 tggccggtgg cgaatacgcc aggcgccaga aggaatgcgc cgacggcatg agatttgtcg
     3061 tgaattcctt ccccacggtg cgcgcggcca gggacgtgtc catggagatg ctggagtcgg
     3121 tcaggcaggg gatgtccgaa gtctctttca gaaggcttcg tcacgcggta agcgaaaacg
     3181 accgtaccgt gagagccgca tcagccctgc aggcgggcga cgtggcggag gtcgggttgc
     3241 tgatgcacgg ctcccacagg agcctggccc atgattacga ggtctcatgt gccgagctcg
     3301 accacctggt cgaaatcgcg tcgcgcatcg atggctgcgc gggagcaagg ctgaccggcg
     3361 ccgg
//
LOCUS       DAOPPA010000367         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30832_length_3351_cov_6.787621, whole genome shotgun sequence.
ACCESSION   DAOPPA010000367 DAOPPA010000000
VERSION     DAOPPA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30832_length_3351_cov_6.787621"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKH54_10645"
     CDS             <1..260
                     /locus_tag="PKH54_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00395.1"
                     /translation="PLGSLSQVRVQENRVRFKGTFRTSVYDMEKLLDKPLSEHKWNKL
                     VLWDYGEKPFRALVGKYYKKSEELGRDPLIGVKVSIWVPRD"
     gene            341..814
                     /locus_tag="PKH54_10650"
     CDS             341..814
                     /locus_tag="PKH54_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00396.1"
                     /translation="MKNAILMMLVAAAAMFAMTCGDEGPNHGFFDEGPGNDNGTHEDS
                     VITDNAALDTDLPVSACLPAWGDQETVTVQPNQAQTFITVESTAMNQLEISDSPLPDR
                     RTGWLAVGTGDCDKAGFLYRDPDLDGISLAKLRYQDVTNVGSLSAQPAPELVVLD"
     gene            859..1875
                     /locus_tag="PKH54_10655"
     CDS             859..1875
                     /locus_tag="PKH54_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00397.1"
                     /translation="MAIAMQPGNDGYQEYRRSEAGIWSGKTVNVVDSGTVTMLTGRGG
                     WQEADGTMVVIATANVDGVALGIVGRLDIIAYSEWVFTSFALPAEYTELLALRHAPDG
                     TAHALYTKTQYPCDGSCDLDLYYGRMASGGTWLEETIQESKWGEPDDEFATNPTMVLD
                     SRGEPVVAATFFKRVITGSIKSAELRLYGRDNGQWCYETVATEVDGYQGSDGTEMTGV
                     SPFVTLDGSGRVHVAFQDKAQWHDSYNQANAVNGQIRYAVRSGKTWTLATLFEQDGEG
                     TGTAQAPLIGMLPTQVAISADGATVYAAGAEFEWDTDSIYNTSGKPMTFKAVVVKAVN
                     LLPE"
     gene            2067..2399
                     /locus_tag="PKH54_10660"
     CDS             2067..2399
                     /locus_tag="PKH54_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00398.1"
                     /translation="MMMTAIFLGGSFNVCLHAPDEHHHFHAHATQSTHGDDCGDTSVH
                     ETDQSDTHRHESEHTACCCACHSSPDISPRLVLTPPADRPDRVMANTVFTMSAALPVP
                     YRPPILSI"
     gene            2478..>3351
                     /locus_tag="PKH54_10665"
     CDS             2478..>3351
                     /locus_tag="PKH54_10665"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD00399.1"
                     /translation="MKRGILVSQALLATALMWACDSGGDYQNQIHNSAREAPATEEHA
                     DDEHADEAHEGHAHAAEGSDLDRPVDELLAESCEHGIKTFMCDECRYEVGVVRVPAAV
                     AAGGLINESSAVTMSFQSDLTLNGEITLDDRRIARLGPREAGFVKKVNVVAGDLVRKG
                     DVLIELESPMYAELTSEYLEASSAVRLAQKTVGWQESLRAKAMTTEREYQETLSTLES
                     ARIRLQSARLALKAAGLSEADAGRAVARSAVTMSIRAPFDGEVFQVNAVRGAHLDYDT
                     EAILMGDQKTLWISI"
BASE COUNT          744 a          966 c         1007 g          634 t
ORIGIN      
        1 ctcccttagg atctttatcc caagttcgtg tccaagaaaa ccgggtgcgg tttaagggaa
       61 catttcgcac ttctgtatat gacatggaaa agcttctgga caagccgctg tccgagcata
      121 aatggaacaa actagttctt tgggattacg gagaaaagcc ttttcgtgcc ctggttggga
      181 aatactataa aaagtcagag gaactcggca gggatccttt gattggggta aaggtttcga
      241 tctgggttcc cagggactga agtctgcatc gccagccggg ttgatgaaat ccgccggtac
      301 ctgctatcat ccagccatct gatgacatgg actgtcagca atgaaaaatg cgattctcat
      361 gatgctggtc gcggccgcgg cgatgttcgc gatgacgtgt ggcgacgagg gaccgaatca
      421 cggcttcttt gacgaaggac caggaaacga caacggcacg catgaagact cggtcattac
      481 cgacaacgcc gcgctggaca ccgatctgcc tgtcagcgcg tgtcttcctg cctggggtga
      541 ccaggaaacg gtaaccgtgc agccgaacca ggcacagaca ttcatcaccg tcgaaagcac
      601 cgccatgaac cagctcgaga tctctgacag tcccctgccc gaccgacgca ccggatggct
      661 ggccgtcggg accggcgact gcgacaaggc cggttttctg tatcgggatc cggaccttga
      721 cggaatcagt ctggctaagc tgcggtacca ggatgtcacg aacgtcggca gtctgtcggc
      781 acaaccggcg ccggaacttg tcgtcctgga ctgaccccgt ccgcgcacaa tgccacgctg
      841 ttttacggcg cgtcctgcat ggcgatagca atgcagcccg gcaacgacgg ataccaggaa
      901 taccgccgca gcgaagccgg gatctggtct ggcaagacgg tcaatgtcgt cgacagtggc
      961 actgtcacca tgctgacggg aaggggcgga tggcaggaag cggacggcac gatggtggtc
     1021 atcgccacgg ccaatgtgga cggggttgca ttgggcattg tcgggcgcct cgacatcata
     1081 gcttacagcg agtgggtctt caccagtttc gccctgcccg ctgaatacac cgaactgctg
     1141 gcactgcgcc acgccccgga cggcactgcc catgctcttt acacaaaaac gcagtatccg
     1201 tgcgatggct cgtgtgacct ggatctgtac tacggcagga tggcgtccgg tggcacctgg
     1261 cttgaggaaa cgattcagga gtccaagtgg ggcgaacctg acgacgagtt cgcgaccaac
     1321 ccgaccatgg ttcttgattc tcgaggtgaa ccggtcgtgg cggcgacctt cttcaagcgg
     1381 gtgatcaccg gctcgatcaa gtccgccgaa ctgaggctgt acggacgcga caacggtcaa
     1441 tggtgttacg aaacggtcgc gaccgaggtc gacggttacc agggctccga tggtaccgaa
     1501 atgaccggag tctcgccgtt cgtgacactc gatggctcgg gccgcgtgca tgtggcgttc
     1561 caggacaagg cacagtggca cgattcatat aatcaggcca atgccgtgaa cggccagatc
     1621 aggtatgcgg tcaggtcggg aaaaacctgg accctggcca ccctgtttga acaggatggc
     1681 gaaggtaccg gcaccgccca ggctcccctg atcggaatgc tgccgaccca ggtggccatc
     1741 tccgcggacg gcgcaacagt ttatgccgcc ggcgccgaat tcgaatggga taccgacagc
     1801 atctacaaca catcgggcaa accgatgaca ttcaaggccg tggtcgtcaa agccgtcaac
     1861 ctgcttcccg aatgaacctg gacgaggttg cttcacttct gtttgactga ataagctcgg
     1921 tcaccaggag acctgtgtcg gcaggcagaa gcatgtgttt cggaattgac acgcaccgga
     1981 tcaattgcca tagtttcacg gcaaacggtt cgttcggaac catggggccg gtattgaaac
     2041 acaaacgtgc gatgacagtg acaacgatga tgatgaccgc cattttcctg ggcggctcgt
     2101 tcaatgtctg cctgcacgcc cctgacgaac atcaccactt ccacgcccat gcgacgcagt
     2161 ctacgcatgg tgatgattgc ggcgacacgt cggtgcatga aactgatcag tccgacacac
     2221 atcgccacga gtccgaacac acggcatgct gctgcgcatg tcacagttca ccggacatct
     2281 cgccgcgcct ggttctgact cctccggctg atcgcccgga ccgggtgatg gcaaacacgg
     2341 tattcacgat gtccgcagcc ctgccggtgc cttatcgacc gccaattctt tccatctgaa
     2401 tcatccagtc acgacacaga cattgcgaac atgacggcgg acggcacgcc ttccgccctg
     2461 gatccggagc aacgatcatg aaacgcggca tactggtttc ccaggctctg ctggcgacgg
     2521 ccctgatgtg ggcatgcgat tccggcgggg actaccagaa ccaaatacat aatagcgcgc
     2581 gcgaggcccc cgccacggaa gaacacgcgg atgatgaaca tgccgatgag gcccacgaag
     2641 gccatgcgca tgcggctgag ggtagcgacc tcgatcgtcc ggtcgatgaa ctgctggccg
     2701 aatcctgcga gcacggcatc aagacattca tgtgcgacga atgcagatat gaagtcgggg
     2761 tcgtacgggt gccggctgcc gtcgccgccg gcggactgat caacgagtca tcagccgtga
     2821 ccatgagctt ccagtccgat ctgactctga acggcgagat cacgcttgac gaccggagaa
     2881 tcgcccgcct tggcccgcgt gaggcggggt tcgtaaaaaa agtaaacgtg gtggccggtg
     2941 acctggtcag aaagggcgat gtcctgattg aactggaaag cccgatgtat gccgagctga
     3001 cgtccgagta tcttgaagct tcttcggcgg tacgactggc acagaagacg gtcggatggc
     3061 aggaatcgct gcgggcgaag gcgatgacga ccgaacggga ataccaggag acactttcca
     3121 cgctggaatc ggcccgaatc aggctgcagt ccgccaggct ggccctgaag gccgccggtc
     3181 tgtcagaggc cgacgctgga cgcgccgttg ccagatcggc ggtaacgatg tcgatccgcg
     3241 ctccgttcga tggcgaggtg ttccaggtca acgcggttcg tggcgcgcac ctggattacg
     3301 acaccgaagc catcctgatg ggtgaccaga agaccctgtg gatcagcatc g
//
LOCUS       DAOPPA010000368         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30984_length_3341_cov_3.797018, whole genome shotgun sequence.
ACCESSION   DAOPPA010000368 DAOPPA010000000
VERSION     DAOPPA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30984_length_3341_cov_3.797018"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKH54_10670"
     CDS             <1..343
                     /locus_tag="PKH54_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00400.1"
                     /translation="RMYREHHFLNDKCRAEFGPGARVADWEDIQRYVRSGRDFEQFLR
                     ENDIHRGNRDRLVYYRGQYKDHRDRHFYLIYNEKGLTPSAVTPIDRAHGNRLNLAGMM
                     HLRSPILCLVP"
     gene            441..1604
                     /locus_tag="PKH54_10675"
     CDS             441..1604
                     /locus_tag="PKH54_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00401.1"
                     /translation="MKNRGLVIMLVVLAAGCADSSGTGSDAYGDTSLAGLPTMPLRTE
                     GRFIVDAEGRRVKLAGYNWNDGEGPMFTMSGLASQHRDDIAQRLARLGFNTIRLVWSN
                     QLVEQNPMVQANLLAANPDLVGKTALEVMDAVVESLGEHGLMVVMNNHISDAIYCCKS
                     DDDNTLWYNDRFPESAWINDWKTIAARYRGHANVIGADLRNEPRLFAYWGPDAGSEMD
                     WAAAAERAGNAVLSEAPDWLIFVEGVNYAQELGGVARRPLEFKVKNRLVYSVHDYPWF
                     FRDESPEEMRAKWERNWAFIFRDQGKAAPVWIGEFGLCNTCFEDNGMNQVWFETFTAF
                     IKDNDLDWCWWVLHEGDTPYGWGVFDEKTNEPWSPGLMEVHRGMIAPTKGPGI"
     gene            complement(1615..1700)
                     /locus_tag="PKH54_10680"
     tRNA            complement(1615..1700)
                     /locus_tag="PKH54_10680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1665..1667),aa:Leu,seq:taa)
     gene            1745..2668
                     /locus_tag="PKH54_10685"
     CDS             1745..2668
                     /locus_tag="PKH54_10685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00402.1"
                     /translation="MDCATDSVAAGLATCDRPARPRFMAIAAILLATAGGCAGHHVRT
                     AVQGTASGSLTACGAGVGLSAELPILADGRFFDLLEAEQVMCPGGAIATLGGDPDKLL
                     SIACPGARIAIYCKADAILPDCVPEPLKTLNPDAARMAAAAVSRIGADRRTDSLKLAK
                     KASDIQPDSEFILAALASAQMANGDFAGAEKTLSRAIGINPDSPALRLAHAVAMSELG
                     DKNSYAAEIARLYDDLPVDHTMRPDLQCRLADNQNRLGNRKVAITMAREACRAGATMC
                     CGMAASDPEPVEVVPWKEPGIGQGDKDQGSM"
     gene            complement(2653..3054)
                     /locus_tag="PKH54_10690"
     CDS             complement(2653..3054)
                     /locus_tag="PKH54_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00403.1"
                     /translation="MKSSVFVIFAFVLVAAGCDRTPEKPANCGPETGAQASCWYEKGC
                     WDLQCEEINKNGTCFTSEGEARMKKICEKEQKGVFYFGCTCPRENSIGGCQNGRQKDR
                     LITTWVYGKNWSADEYRNVCESTAYDRYIEP"
     gene            complement(3116..>3341)
                     /locus_tag="PKH54_10695"
     CDS             complement(3116..>3341)
                     /locus_tag="PKH54_10695"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939506.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HOD00404.1"
                     /translation="PIVARLTSAPGNDAPIGGLKVVAPDGWFAARPSGTENIYKIYAE
                     SLVGQDHLDRILAEARDMVDAVLDGSGAPM"
BASE COUNT          692 a          998 c         1019 g          632 t
ORIGIN      
        1 gcgcatgtac cgtgaacacc atttcctgaa cgacaagtgc cgcgccgaat ttggtcccgg
       61 agcacgggtc gccgactggg aagacattca aaggtacgtc cgttcgggac gcgacttcga
      121 acagttcctg cgggaaaacg acatacaccg cggcaatcgc gaccggctgg tttattaccg
      181 gggtcagtac aaggatcacc gcgatcgtca tttttatctg atctacaacg aaaaaggcct
      241 tacgcccagt gcggttacgc cgatcgatcg tgcccacggg aaccggctga acctggccgg
      301 catgatgcac ctgcggtcac cgatcctctg cctggttcct tgacgatcaa tccacaaatc
      361 gtccgggggc gatgcgaacc cggacgggaa gacactcggc tactttgcga tcccgacggc
      421 gacattttca ggtgaatgaa atgaaaaacc ggggcctggt aatcatgctt gttgtcctgg
      481 ccgccggttg cgcggattcg tccggtactg gcagcgatgc gtacggggat acttccctgg
      541 caggacttcc gacgatgccg cttcggactg aaggtcgctt catcgtggac gccgaaggcc
      601 ggcgggtcaa gcttgcggga tacaactgga acgatggcga aggtccgatg ttcacgatgt
      661 ccgggctggc ctcgcagcac cgcgacgaca ttgcccaaag gctggcgcgg cttggattca
      721 acacaatccg gcttgtctgg tccaaccagc tcgtcgaaca gaatcccatg gttcaggcga
      781 acctgctggc cgccaatccg gatcttgtcg gaaagacggc gctcgaagtc atggacgcgg
      841 tggtcgaatc gcttggcgaa cacggattga tggttgtaat gaacaaccac atatcggacg
      901 ccatctactg ctgcaagagt gatgacgaca acacgctgtg gtacaacgac cgctttccgg
      961 aatccgcctg gatcaacgac tggaagacga tcgccgcaag gtatcgtggt catgccaacg
     1021 tcatcggcgc cgacctgcgc aacgaaccca ggcttttcgc gtactgggga ccggatgccg
     1081 gcagcgaaat ggactgggcc gcggcggccg agcgcgcggg aaacgccgtt ctatcggaag
     1141 ccccggactg gctgatattc gtcgagggtg tcaattacgc ccaggaactt ggcggcgtcg
     1201 cccgtcgccc gctggaattt aaggtgaaga accggctggt gtattcggtt cacgactatc
     1261 catggttctt cagggacgag tcgcctgaag aaatgcgggc gaagtgggaa cggaactggg
     1321 ccttcatctt cagggatcag ggtaaggctg ccccggtatg gatcggcgaa ttcggcctgt
     1381 gcaacacatg ctttgaagac aacggaatga accaggtctg gttcgagacg ttcacggcct
     1441 tcatcaagga caacgacctg gactggtgct ggtgggtgct tcatgaaggc gacacgccat
     1501 acggatgggg cgtctttgat gaaaagacga acgaaccgtg gtcgcccggc ctgatggaag
     1561 ttcatcgcgg catgattgcc ccgacgaagg gaccgggcat ttagattcaa gggctggtgc
     1621 caagggcggg actcgaaccc gcacgccctt gcggacaagg gattttaagt cccctgcgtc
     1681 tgccatttcg ccaccttggc gcgactgaag tatgcaccag gaatgccaca caaggaagat
     1741 gcttgtggac tgcgcgacgg attccgtggc ggctggcctg gcaacctgcg accgcccggc
     1801 ccggccgcgg ttcatggcga ttgccgcaat tcttcttgcg actgcgggcg gatgtgccgg
     1861 acatcatgtc aggacggcgg ttcagggaac cgcgtcaggt tccttgaccg catgcggggc
     1921 gggcgtcggg ctgtcggccg aactgccgat actcgccgac ggccgcttct tcgacctgct
     1981 tgaagcggaa caggtcatgt gtcccggcgg cgcaattgcg accttgggtg gcgatccgga
     2041 caagttattg tcaatcgcct gtcccggcgc gcggattgcg atttattgca aagccgacgc
     2101 aatcctgccc gactgcgtcc cggaacccct caaaacgctc aatcccgatg ccgcccgaat
     2161 ggccgccgca gcggtttcgc gaatcggcgc cgatcgccgg accgattcgt tgaagcttgc
     2221 gaaaaaggca tctgacatcc agcccgattc ggaattcatc ctggctgccc tggcatcggc
     2281 ccagatggcg aacggggatt tcgcgggcgc ggaaaagacg ctttcacggg cgatcggcat
     2341 caacccggat tcgccggccc tgcggctggc acacgccgtc gcgatgtccg aactgggcga
     2401 caagaactcg tatgccgccg aaatcgcgcg actgtacgac gatctgccgg tcgaccatac
     2461 gatgcgcccc gaccttcagt gcaggcttgc cgacaaccag aaccggctgg gaaaccggaa
     2521 ggtggcaatc acgatggccc gcgaggcgtg ccgggcgggc gccacgatgt gctgcggcat
     2581 ggcggcaagc gatcccgaac ctgtggaagt ggtgccttgg aaggaaccgg gaatcgggca
     2641 aggcgataag gatcagggct cgatgtagcg atcgtatgcg gtcgattcgc aaacgttgcg
     2701 gtattcgtcg gcggaccagt tcttgccata gacccaggtg gtgatcaggc ggtccttctg
     2761 cctgccgttc tggcacccgc cgatcgagtt ttcacgcgga catgtgcagc cgaagtagaa
     2821 gacgcccttc tgttccttct cgcagatctt cttcatccgg gcctcgcctt ccgacgtgaa
     2881 gcacgtgcca ttcttgttga tctcttcgca ctgaagatcc cagcaaccct tttcatacca
     2941 gcacgaagcc tgggcaccgg tctcggggcc gcaattggcc ggcttttccg gtgtccgatc
     3001 acacccggcc gcaacaagca cgaacgcgaa aatcacgaaa acagacgact tcacatcacc
     3061 acccgcagaa aaagcaggac ggaacattaa cacataagtt caggcatgtc gccaactaca
     3121 tcggcgcgcc ggaaccatca agaacagcat cgaccatgtc tcgtgcctcg gccaggatac
     3181 gatccagatg atcctgcccg accaggcttt ccgcatagat cttgtagatg ttttccgtgc
     3241 ccgacggccg cgccgcgaac cacccgtccg gggcgacgac cttcaacccg ccgatcggcg
     3301 cgtcgttgcc cggcgcgctg gtcagtctgg ccacgatggg c
//
LOCUS       DAOPPA010000369         3340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_30995_length_3340_cov_3.996956, whole genome shotgun sequence.
ACCESSION   DAOPPA010000369 DAOPPA010000000
VERSION     DAOPPA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30995_length_3340_cov_3.996956"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKH54_10700"
     CDS             <1..846
                     /locus_tag="PKH54_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD00405.1"
                     /translation="GTPFMHDYHPLKELAPRDIVARAIDHEMKMSGHDHVLLDMTARS
                     PDYIENRFPNLTATCRTFGYDLPTEPVPVVPAAHYMCGGVRTDLDGRTSIPGLFAVGE
                     VACTGLHGANRLASNSLLEAVVFGRRAAAAVMECPDRVRTPDDAPDPPSWMRDTAVSS
                     NVENVMVTQDWDEVRRLMWNYVGIVRSNRRLNAARRRLDLVSREIRDYYLQSPLTPDL
                     VELRNIAGVANLIVRSALSRQETRGLHFNVDYPETDDRRCLHDTEIWLGVNTRVQKLG
                     PTRGE"
     gene            complement(1231..2343)
                     /locus_tag="PKH54_10705"
     CDS             complement(1231..2343)
                     /locus_tag="PKH54_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00406.1"
                     /translation="MSDKAVETGGSDKPRRRWAATVALSVLGVILAGLMVYWLKPPVD
                     ATRDFKVPKLTSAFVDGEPFMRLQLAPGDAYRPIEVMEGTNIKVECFAVKTESTKLRF
                     SLEGFGRKVDSADCVFNVDVTDAVGSFSDLRISYHDGDTQLIDTLTIPVVVVAKSERI
                     EFHQLQDANHQPVAAGGVPDRIFVYGRAITNLPGDTSEYGALFFVADPANDVPVVQMM
                     PLLQGDQPKPMIGRIVKYRNYGKDLYGYAMWSPEPVNISPIHRTITDLYIGIFKLADI
                     DQIFSKLLKVEITGPDSVMVTPQVASPYDVMQMTVGQKLLSESFHVVAGAGRAKIDER
                     VKATAEKAIENIIVNTPKADSAPVEGAPEPGTAPSN"
     gene            2522..>3340
                     /locus_tag="PKH54_10710"
     CDS             2522..>3340
                     /locus_tag="PKH54_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00407.1"
                     /translation="MRDHISDEEIREIHNRAGQFSLLLVKALLQTGTYDASHPIARAA
                     TADMFAAFSSLAQDTDELTYLLLSTVDGDGILVDGLLSEPIPIKSVFRGMMGDHFLNK
                     FHDYFARNRIASFSIKTSISAESFTQFLAYLTAWGGEGVVGHRSAEEFSRELLELGLF
                     DVTIISIDELVGGKRHLAWPVKIALSRMRKDISKLPMLRNADRAEVQRLKIAAMSDII
                     RPIKRPDTLRDLMLNADLVADGIQITSTDEVEDTLITAMTADAAFDVAEALLAIK"
BASE COUNT          701 a         1018 c          912 g          709 t
ORIGIN      
        1 ggcacgccgt tcatgcacga ttaccatccc ttgaaggaac tggctccacg agacattgtt
       61 gcccgtgcca tcgaccacga aatgaagatg tccggacatg atcacgtgct tctggacatg
      121 acggcgcgtt caccggatta catcgagaat cgattcccga acctgacggc cacctgccga
      181 acgttcggct acgatttgcc cacggaaccg gttccagtcg tgccggcggc ccattacatg
      241 tgcggcggcg tgcggacgga tctggacgga aggacatcca tccctggtct tttcgccgtt
      301 ggcgaggtcg cctgcaccgg tctgcacgga gcgaacaggt tggcatcgaa ttcgttgctc
      361 gaggccgtcg ttttcggccg tcgggccgcg gccgcagtga tggaatgccc agacagggtg
      421 cgtacccccg acgacgctcc cgatccgccg tcctggatgc gcgacaccgc tgtttcatcg
      481 aatgtcgaaa acgtcatggt cacccaggac tgggacgagg ttcgccgcct gatgtggaac
      541 tatgtcggga ttgtccgttc aaaccgccgc ctgaatgcgg cccgccgccg gcttgacctg
      601 gtcagtcgcg agatccggga ctattacctt cagtcgccgc ttacaccgga tctggtcgaa
      661 cttcgcaaca tcgcgggagt cgcaaacctg atcgtccgca gcgccctgag tcgccaggaa
      721 acccgtggcc ttcacttcaa tgtcgattat cccgaaaccg atgaccgccg ctgccttcac
      781 gacaccgaga tctggcttgg cgtcaacacc cgtgtccaga aacttggccc cacccggggc
      841 gaatgaaaat tcgcccgccg ggcagatgcg atccgccccc agatccgtaa gggcgaatga
      901 aaattcgccc gcctgcgtga agattcgccc gtgggccctt cgcttcgctc aggggacgag
      961 gagctgccgt acgccccttg ttccttgcca ctgggggtcc ttcggggtta cccgtcccgg
     1021 gaatcgcggt cgcgcacggg gccagccccg tgtcgcgccg ctgcctctcg gcgcgcgacg
     1081 gtcattacgc cccgcaaggt gtcgcgcgcc tgcgagagct tcccggctac gggtacccgc
     1141 tcggccccca tgctccacgc cctcaattcg taagggcgaa tgaaaattcg cccgccgcgg
     1201 ttcagtggac tgaagttaat gcgggacggt tcagttggat ggggccgtgc ctggttccgg
     1261 ggcgccttcg acgggcgcgg agtccgcctt gggtgtgttt acgatgatat tttcgatcgc
     1321 cttctcggcg gtcgccttca cgcgttcatc aatctttgcc ctgcccgccc ccgcaacaac
     1381 gtggaatgac tcagaaagca gcttctggcc caccgtcatc tgcataacat catatggcga
     1441 cgcaacctgg ggcgtaacca tcacactgtc tggcccggta atctcaacct taagcagctt
     1501 cgaaaaaatc tggtcgatat ctgccagctt aaaaatcccg atatagagat cagtgattgt
     1561 ccggtgaatc gggctgatat tcacgggttc tggcgaccac atcgcgtaac cgtacaaatc
     1621 cttgccatag ttacggtact tgacgatacg gccgatcatc ggcttcggtt gatcgccctg
     1681 aagcagaggc atcatctgaa ccacgggcac gtcgtttgcc ggatccgcca cgaaaaacag
     1741 cgcgccatat tccgacgtat ctccgggcag gttcgtgatc gcccttccat aaacgaaaat
     1801 tctgtccggc acgcccccgg ccgcgacggg ctgatgattg gcgtcctgaa gctgatgaaa
     1861 ttcaatccgc tcgctctttg cgacaacaac aactggaatt gtcagagtgt cgatcagttg
     1921 cgtgtcgcca tcgtgataac tgattcgaag gtcgctgaaa ctccccacag cgtccgtaac
     1981 atcgacgttg aaaacgcaat cggccgaatc gacctttcgg ccgaagccct ccagggaaaa
     2041 tcggagcttc gtgctctcgg tcttgactgc gaaacactcg accttgatgt tcgttccttc
     2101 cataacctcg atgggacggt acgcgtcacc gggggcaagt tgcagcctca tgaaaggctc
     2161 gccatcgacg aaggcggacg tcagcttcgg aaccttgaag tctcgggttg cgtcaaccgg
     2221 cggcttcaac cagtaaacca ttaacccggc aaggataacg ccaagtaccg aaagagccac
     2281 ggttgcagcc cagcggcgcc gcggtttgtc cgatccacca gtttcgacgg ccttatccga
     2341 catcttatga cctccagaag acgaagaaga atacggcgat atcgcgtctt tggcaattgt
     2401 gccagtcagt gctacatcac atgcaaagat ttcaggatct tacggattta cacatgcttt
     2461 cttgacaaac cgccggccag cacgaagaat tgtttgaagc gccatcacgg ataaatcggt
     2521 aatgcgtgat cacatatcgg acgaggaaat ccgggagatc cacaatcgcg ccggacaatt
     2581 ctcgttgctt ctggtgaagg ctcttcttca gaccgggaca tacgacgcaa gtcatcctat
     2641 cgccagggcc gcgactgcgg acatgttcgc tgccttttcc tcactcgccc aggacacgga
     2701 cgaactgacc tacctgcttc tttcaaccgt tgacggtgat ggcattcttg ttgacggcct
     2761 gttgagtgaa ccaattccca tcaagagcgt tttccgcggc atgatgggcg accatttcct
     2821 taacaagttt cacgactact tcgcccgcaa ccggatcgcg tcgttttcga tcaagacgtc
     2881 catttcggcc gagtccttca cccagttcct tgcctacctg acagcctggg gtggtgaagg
     2941 cgtcgtcggc catcgatccg ccgaggagtt ttcgcgcgag ctgcttgaac ttgggttgtt
     3001 tgacgtaacg atcatcagta tcgacgagtt ggtgggcggg aaacgtcacc tcgcgtggcc
     3061 cgttaagatc gcactgtcca gaatgcgcaa ggacatcagc aagcttccga tgctcaggaa
     3121 cgctgaccgc gcggaagttc agcggcttaa aatcgcggcg atgtccgaca tcatccggcc
     3181 aatcaaaaga cccgacacgt tacgtgattt gatgctcaat gcagatctgg tagccgacgg
     3241 catccagata accagcaccg atgaagttga agatacactt attacggcaa tgaccgccga
     3301 cgccgccttc gacgtggcag aagcgcttct tgccatcaag
//
LOCUS       DAOPPA010000370         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31072_length_3335_cov_3.596951, whole genome shotgun sequence.
ACCESSION   DAOPPA010000370 DAOPPA010000000
VERSION     DAOPPA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31072_length_3335_cov_3.596951"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKH54_10715"
     CDS             <1..964
                     /locus_tag="PKH54_10715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD00408.1"
                     /translation="KVLHRHLLTDEGQVHRFHREAKAASRLHHPNAIGILDFGRTGDG
                     WFYIAMEYLNGKDLSRMANKEGAMPVEKVIHVARQVLDALDEAHASNVVHRDLKPENI
                     MVEPLRSDPLFVKVLDFGIAKIRDTDGSDASGFKTATGMVFGTPEYMSPEQIRGDELD
                     GRSDLYSLGIVMYQMLTGQLPFTGETVIEIATAHLINPVPNMRPIRPDIPEKMIRLVE
                     KLIEKKRENRFQTAEETRKNLDDVADLLRRKKDELKNGLDAAVLDGGGASGLDMRTAD
                     TITTTSINLGGGMLHLTYPVVAAVAMGLILIGGGFAWALSLFLH"
     gene            1044..1850
                     /locus_tag="PKH54_10720"
     CDS             1044..1850
                     /locus_tag="PKH54_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOD00409.1"
                     /translation="MDSPNTATEREIKLRIDGRDDYRRLAGVLPVPRRWGMQLNAYLD
                     TPDSLLRNVRTSLRVRITPDHARLTMKSPWSAGFLDGSVVVTPAGFEPVTGHAHPDPR
                     AVSWERDSSGAFVHRETEVQIDRALAIRWICDPSLATPWLFEGFEGLRDQTGDGGLRL
                     VCWSLTRRAICDVPEGLTIELDETVFPDGFRDFEVELEHPDSERALKVIMEYAAGAGI
                     SLHHQEFSKHARARAHAGRLDWVIPSGASTDAQPPLFMEPAPTGMFNGCR"
     gene            1867..>3335
                     /locus_tag="PKH54_10725"
     CDS             1867..>3335
                     /locus_tag="PKH54_10725"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD00410.1"
                     /translation="MTSCAGYNRSIYGMAIAGLITAGMSLAAYVLFIPAVVYWLDGPE
                     FIAAAGNLGIAHPPGHPAALIPMKLFMLLPAGDAAFRANLFSAFFGAVSAGLVACLSF
                     IAVAGRRSDAWASVVAIASGLMFGLCRSAFIQAMAVEVYTLNTALVLGALVVVMAGPL
                     AGRRPDDARNGVAAGVLLGLALANHHYLAVLAIPALVAAFWCDSRSVRPLAVVMFVTA
                     CTTIAAMMYLPIRSAAGAWPSWAAIDSVGDFLWYASATIFSQSVGAVHGAAQVADKLP
                     NGILAFVLVGESLGPIVPVMALGGLYLMARLGFRRVALVLFLLGAGGFASKVLMGITD
                     PSNPDDHGYFMALLAALAIAAPQLLAVPGGDGHASRRFAIPANLVALVLVAFCAVGGF
                     RIGSNRQAFDHTDRVADIVWDSIPQSGIAFISHYPVFFLMQYGQEIEGRRPDVTLVQQ
                     SLYSKSLGGRDYAAAIGRRDPGTLPLVDAFIRDGTLSWPL"
BASE COUNT          562 a          952 c         1049 g          772 t
ORIGIN      
        1 gaaggttctg cacaggcacc tgcttaccga tgaaggccag gtgcatcgct tccaccgtga
       61 agccaaggcg gcatcgagac tgcatcaccc caacgccatc ggaatcctgg atttcgggcg
      121 cacgggggat ggctggttct acatcgcgat ggaatacttg aacggcaagg atctttcccg
      181 catggccaac aaggaagggg cgatgccggt cgaaaaggtt attcatgtcg cgcgccaggt
      241 tctggacgcc ctggacgaag cccacgcttc aaacgtcgtg catcgcgacc tgaagccgga
      301 aaacatcatg gtcgagcctt tgcgctcgga tccgcttttc gtgaaggtcc ttgacttcgg
      361 catcgcaaag attcgcgaca ccgacggcag tgatgccagc ggattcaaga ccgccacggg
      421 aatggtgttc ggaaccccgg agtacatgtc tcctgaacag attcgcggtg acgaactgga
      481 tggtcgttcc gatctgtatt cccttggaat cgtgatgtac cagatgttga ccggccagct
      541 tccgttcacc ggcgagacgg tgattgagat cgcgacggcg catcttatca atccggtacc
      601 gaacatgcgg ccgattcgtc cggatattcc cgagaagatg attcgcctgg tcgagaagct
      661 gatcgaaaag aagcgcgaga atcggttcca gaccgccgaa gagaccagga agaaccttga
      721 tgatgtggct gacctgctgc ggcgaaaaaa ggacgagttg aagaacggac tggatgccgc
      781 ggtgcttgat ggtggcgggg cttccggtct tgacatgaga acggccgata ccataacaac
      841 gacgtccatc aatctgggcg gcggcatgct tcatcttaca tacccggttg ttgctgccgt
      901 ggcgatggga ttgattctca tcggtggcgg attcgcctgg gcgctttcgc tcttcctgca
      961 ctgatttgca ttgggccgcg cgtctgctgc ccggattggc cggggcgccg gaaccagccg
     1021 ggcccgatgg tcggggaacg tcgatggatt cgccaaacac cgccaccgag cgcgagatca
     1081 agttgaggat cgacgggcgg gatgattatc ggcgtctggc cggcgtgctg ccggttcccc
     1141 gtcgctgggg catgcagctt aatgcatacc tggatacccc ggattcactg cttcggaatg
     1201 tgcgtaccag tcttcgtgtc cggattacgc ccgatcacgc ccgcctgacg atgaagagtc
     1261 cctggagcgc cggttttctg gatggcagcg tggtggttac cccggccggt ttcgagccgg
     1321 tcaccggtca cgcgcatccg gatccgcgcg cggtttcatg ggagcgtgat tcttctggtg
     1381 cattcgttca tcgcgaaacc gaggtgcaga ttgatcgcgc ccttgcgatt cgctggattt
     1441 gcgatccttc acttgcaacg ccatggctgt tcgaaggatt cgaggggctg cgcgaccaga
     1501 ctggtgatgg tgggcttcgc ctcgtctgct ggtcgctgac gaggcgggcg atctgcgatg
     1561 tgccggaggg attgacaatc gaactggacg agacggtttt tcctgacggt tttcgcgact
     1621 tcgaagtcga acttgaacat cccgattccg aacgggcgct taaggtcata atggaatacg
     1681 cggcaggcgc cgggatttcc cttcatcacc aggaattttc gaaacatgcg cgggcgcggg
     1741 cgcatgccgg gcggctggac tgggtaattc cgtccggcgc atcgacggac gcgcagccac
     1801 cgctctttat ggaaccggcg ccgaccggga tgttcaatgg ctgccggtga ccggggtacg
     1861 cggcttatga cttcatgtgc cggttacaat agatcgatct acgggatggc aatcgccggt
     1921 ctcattacgg caggaatgtc cctggccgcg tacgtccttt tcattccagc ggtcgtctat
     1981 tggcttgacg gtcccgagtt catcgctgcg gctggaaacc ttggcatcgc ccatccgccc
     2041 ggtcatccgg ccgcccttat cccgatgaag ctgttcatgc ttctgccggc gggtgacgcc
     2101 gcgttcaggg caaacctctt ttcggcgttc ttcggtgccg tgtccgcggg gcttgtcgca
     2161 tgcctgtcgt ttatcgcggt tgccggtcga aggtcggatg catgggcgtc agtggtcgcg
     2221 atcgcgtccg gacttatgtt cggactgtgt cggtccgcat tcatccaggc aatggctgtc
     2281 gaggtttata cgctcaacac ggccctggtg cttggcgcgc tggtcgttgt gatggccggg
     2341 ccgcttgcgg ggcgtcgtcc cgatgatgcg cgtaacgggg tggccgccgg cgtcctgctg
     2401 gggcttgcgc ttgcaaacca tcactatctt gcggtgctgg cgatcccggc gctggttgcc
     2461 gccttctggt gcgattcgcg ttccgtccgc ccgcttgcgg ttgtgatgtt tgtgaccgcg
     2521 tgcacgacca tcgcggcgat gatgtatctt ccaatccgca gcgcagccgg ggcctggcct
     2581 tcatgggccg ccatcgacag cgttggcgat tttttgtggt atgcgtcggc aaccatcttc
     2641 agccagtccg tcggtgccgt gcatggtgcc gctcaggtcg cggacaaact gcccaacggc
     2701 atccttgcct ttgtcctggt cggtgaaagc cttggcccga ttgttcccgt gatggccctt
     2761 ggcggcctct atctgatggc acggcttggc tttcgccgtg tcgcgctggt tcttttcctc
     2821 cttggggccg gaggtttcgc gtcgaaggtc ttaatgggaa tcacggatcc ttcaaatccc
     2881 gacgatcacg ggtatttcat ggccctcctg gcggccctgg cgatcgcggc gccgcagctt
     2941 ctggcggttc cgggtggaga tggccacgca tcgcgtcgat tcgcgattcc cgccaacctg
     3001 gttgcactcg ttcttgttgc gttctgcgct gtcggcggtt tcaggatcgg ttcaaatcgc
     3061 caggctttcg atcatacgga tcgggtggcc gacattgtct gggactcgat tccgcaatcg
     3121 gggattgcat ttatttcaca ctacccggtt ttctttttga tgcagtacgg ccaggaaatc
     3181 gaaggcaggc ggcccgacgt gacattggtt caacagagtc tttacagcaa gtctcttggc
     3241 ggccgtgatt atgcagctgc catcggacga agggatcccg gcactcttcc cctggtcgat
     3301 gcgttcatcc gggatggaac cctgtcatgg ccgct
//
LOCUS       DAOPPA010000371         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31080_length_3334_cov_4.023788, whole genome shotgun sequence.
ACCESSION   DAOPPA010000371 DAOPPA010000000
VERSION     DAOPPA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31080_length_3334_cov_4.023788"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKH54_10730"
     CDS             <1..859
                     /locus_tag="PKH54_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOD00411.1"
                     /translation="VNVAYEAATADLRDINLIDPFHLEAYGKQAVNYNRDVEAFPIVR
                     RILARIMGDDAVYRSPTDMGVNRAGFGIVNDLVCQQASKQEIIRRYFRYSCEYMMGLA
                     DKESVQRCEMLMNEMDIVPLDRPMVQPARNAAENAETEGMAGGDVCCGAALELPDGSI
                     VTGHNSPTMHAASAVVLNAIKKLAGLPQHLHLLSPNIIESIGTLKKSVLREKNISLDL
                     KETMITLGICATTNPTAGIAMDKLKELNGCEMHMTHIPSPGDEAGLRRLGINLTTDPA
                     FASKDLFIS"
     gene            complement(897..2387)
                     /locus_tag="PKH54_10735"
     CDS             complement(897..2387)
                     /locus_tag="PKH54_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD00412.1"
                     /translation="MERILIKNARIVDVQAGKHLPEGTGILIEGDRIQAMPMLEGPGE
                     KPALPGDLPDCRVIDAGGRTLIPGMFNTHCHLHMGPRGIVGARQTVKSLADCIDRGVT
                     NIRDALCYDLRDSREWSGRIAKGSVAGPRIQQAVHISIEGGTYAPVRHPKTLLQLAAI
                     GLRPLDYERVESGVITLRPDAPPQDFRDAVNRAIDLRGAGAIKLCDQPEHFLSYQPGA
                     PLLSQAQLDAAVDQARVRGVPATMHNVTAAGFRRAVRSGMTSTAHIPLDALLDQADID
                     AFMTGQTMFEPTLSLTYFLCYHLRGSRLCGDPEIDRLDAMRSPTYERVIDESWIPELR
                     PFRMGIHRMLAAGNPKVLGLIDVGTPFNYYQPIVTTGAENVRRLIDAGAAARLSCGTD
                     AGASNCGPAAIDLEMTMLDFALNRPGRNPLAGADFLRTATINSARSLGLEADFGTLEP
                     GKVADIAVLDGNPLADWKLIGRPVAALFKAGRLVVNRCGLHFDCRS"
     gene            2590..3321
                     /locus_tag="PKH54_10740"
     CDS             2590..3321
                     /locus_tag="PKH54_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00413.1"
                     /translation="MFFRFVAVLGALVTILWVGFAFAKPSTELCEKFFTPAVPGEKVL
                     ACKYVGGDRPTRELNGQQYLTDVGTCWDFDLTAVQNCGCRLFMKSSICCRNGSTTECE
                     WRVGDSKLVDCKPFGQPQFGLGGATESEACRLRRAAADCYKDKNLSFAGVTVGPCMVG
                     PRFVCPKGDENLSEFLDGRCGPTPEDCGCVLAEPCSKKDEIDCYKAWKADPEAVKCFD
                     PKVYSNGWLRSECYRKLQESRVPAP"
BASE COUNT          604 a          989 c         1081 g          660 t
ORIGIN      
        1 ggtcaacgtg gcgtacgagg ccgccactgc cgacctgcgc gacatcaacc tgatcgatcc
       61 gttccacctc gaggcgtacg gcaagcaggc cgtcaattac aaccgcgacg tcgaggcgtt
      121 cccgatcgtc cgcaggattc ttgcccggat catgggtgac gacgccgtct atcgcagccc
      181 gaccgacatg ggcgtcaacc gcgccggctt cgggattgtc aacgacctgg tctgccagca
      241 ggcatcgaag caggaaatca tccgccgcta cttcaggtac tcgtgcgaat acatgatggg
      301 ccttgccgac aaggaatcgg ttcagcgttg tgaaatgctg atgaacgaaa tggacatcgt
      361 gccgctggat cgcccgatgg tgcagccggc ccgcaacgcc gcagagaatg ccgagaccga
      421 gggcatggca ggtggcgatg tttgctgcgg ggcagccctg gaactgccag atggaagtat
      481 cgtgaccggt cataactcgc cgacgatgca cgcagcatcg gccgtggtcc tgaacgcgat
      541 aaagaaactg gccggactgc cgcagcacct gcacctgctt tctcccaata tcatcgagtc
      601 gatcggcacc ctgaagaagt ccgtgctgcg tgaaaagaac atcagcctgg atttgaagga
      661 aacgatgatc acgctgggta tctgcgcgac cacgaacccg acggccggca tcgccatgga
      721 caagttgaag gaacttaatg gctgcgagat gcacatgacg cacatcccgt cacccggcga
      781 cgaggccggc ctgcgccgcc tgggcatcaa cctgaccacc gaccccgcct tcgccagcaa
      841 ggacctgttt atttcctgac gctttgcact tgagtctcaa taagtggcgt ttggcgctaa
      901 cttctgcaat cgaaatgcag tccacagcgg ttgacgacaa ggcggccggc cttgaacaat
      961 gcggcgaccg ggcgtccgat gagtttccag tcggcaagcg ggttgccgtc caggaccgcg
     1021 atgtcggcca ccttgccggg ctccagcgtt ccgaagtccg cttcaagacc caggctgcgg
     1081 gcgctgttga ttgtcgcggt ccgcaggaaa tcggcgccag ccaggggatt gcggccggga
     1141 cggttcagcg cgaagtccag catcgtcatt tcaaggtcga tggcggcggg gccgcagttc
     1201 gaggcgccgg cgtccgttcc gcaggaaagc ctggcggccg ccccggcgtc aatcaggcgg
     1261 cgcacgtttt cggcgcccgt ggtgactatc ggctggtagt agttaaatgg tgttcccacg
     1321 tcgatcaggc ccagaacctt ggggtttccg gcggccagca tcctgtggat gcccatcctg
     1381 aatgggcgca gttcgggaat ccacgattcg tcgatgacgc gttcgtaagt gggcgaccgc
     1441 attgcgtcca gccggtcgat ttcggggtcg ccgcaaaggc ggctgccgcg aaggtggtaa
     1501 caaaggaagt acgtcagtga aagggtcggc tcgaacatgg tttgtccggt catgaacgcg
     1561 tcgatatcgg cctggtccag gagcgcgtcc agcgggatgt gcgccgttga agtcatgccc
     1621 gagcggacgg cccggcggaa tccggcggca gtaacgttgt gcatggttgc cgggacgcca
     1681 cgcacccttg cctggtccac ggccgcgtca agctgggcct gtgacagcag gggcgcgccc
     1741 ggctggtatg agaggaagtg ttccggctgg tcgcaaagct tgattgcccc ggcgccgcgc
     1801 aggtcgatgg cccggttcac ggcgtcccgg aaatcctggg gcggcgcgtc cggacgcagc
     1861 gttatcaccc cggattcgac acgttcgtag tccaggggcc gcagaccgat cgccgcaagc
     1921 tgcagaagtg ttttcgggtg cctgaccggt gcgtacgtcc cgccttcgat gctgatgtgc
     1981 accgcctgct ggatgcgcgg gccggcgacg gatcccttgg cgatccgccc tgaccactcc
     2041 cggctgtccc gcaggtcata gcacagcgcg tcccggatgt tcgtgacgcc gcggtcgatg
     2101 cagtcggcca gggacttgac cgtctgcctg gcccccacga tgccacgtgg gcccatgtgc
     2161 agatggcagt gtgtgttgaa catgccgggg atcagggttc ggccgccggc gtcgatgacg
     2221 cggcagtccg gcaggtcgcc cggcagtgcg ggcttttcgc ccgggccttc gagcatcggc
     2281 attgcctgga tgcggtcgcc ttcgatcaga attccggtgc cttcgggcag gtgtttcccg
     2341 gcctggacgt ccacgattct tgcgttcttt atcaggattc tttccatcgt cccgctccgg
     2401 gtgtgcctgg caaatcatcg cgcaacgcgt catccaatgt ccagcgctac gtctgattca
     2461 atgatttgtg caaagtgatt cacgaatccg gcgctcgaga agacttagtt gacacgaatt
     2521 cgtaccggca tgggttagaa tcaggccatc ccgaaaccgt ccggcagggt ggccggcagg
     2581 agcaggacca tgtttttccg gtttgttgca gtgctgggtg cgctggtaac gattctttgg
     2641 gtcgggtttg ccttcgcgaa gccctccacc gaactttgtg aaaagttctt tacgccggcg
     2701 gttcccggcg aaaaggttct ggcgtgcaag tacgtcggcg gtgatcgtcc aaccagggaa
     2761 ctgaacggcc agcagtacct gaccgacgtc gggacttgct gggatttcga cctgacggcc
     2821 gtccagaact gcgggtgccg gctgttcatg aagtccagca tctgttgccg taacggttcc
     2881 acgaccgagt gcgaatggcg cgtcggcgat tcgaagctgg ttgattgcaa gccgttcggc
     2941 cagccgcagt ttggccttgg cggggcgacc gagtccgagg cgtgccgtct gcgtcgtgcg
     3001 gcagccgatt gttacaagga caagaacctg tcgtttgccg gggttaccgt cgggccttgc
     3061 atggttgggc cccgttttgt ctgtccgaag ggcgatgaaa acttgtccga gttccttgac
     3121 gggcgatgcg gacccacacc ggaggattgt ggctgtgtgc tggccgagcc gtgctcgaag
     3181 aaagatgaaa tcgactgcta caaggcttgg aaggccgacc ccgaggccgt gaagtgcttt
     3241 gaccccaaag tgtattcaaa cgggtggctt cgcagtgaat gttaccgcaa gctgcaggaa
     3301 tcgcgcgttc ctgccccttg acgccgtttg ctgc
//
LOCUS       DAOPPA010000372         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31214_length_3322_cov_4.123355, whole genome shotgun sequence.
ACCESSION   DAOPPA010000372 DAOPPA010000000
VERSION     DAOPPA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31214_length_3322_cov_4.123355"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKH54_10745"
     CDS             complement(<1..621)
                     /locus_tag="PKH54_10745"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOD00414.1"
                     /translation="MDFTPHTEAEIAEMLRVIGVSSIEELFAVIPSEFKVKGCMNLPE
                     SMSEADVLARMKELAALNMTADQCPSFLGGGAYNHYVPAAIHHLVGRGEFLTSYTPYQ
                     PELSQGTLQAQFEYQSMVAQILGMDVSNASMYEGATAMTEACLMAQKHTRRDILAVSS
                     LVHPQYIDVLRTYTGAGEIVMIPAAPASTTDMDAARRLVGDNVAAVV"
     gene            complement(689..1084)
                     /gene="gcvH"
                     /locus_tag="PKH54_10750"
     CDS             complement(689..1084)
                     /gene="gcvH"
                     /locus_tag="PKH54_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD00415.1"
                     /translation="MSIPKEYRFTDTHEWVKVEEDNIVSIGITDYAQDHLGDIVSVEA
                     PRVGDEIEKGEPIGMIDSQKASTEVFAPLTGLVLESNDLLEDDPSVMNSDPYEEGWLV
                     RVEVADMEQLDELMTAEDYEEYLGRLEEE"
     gene            complement(1168..2274)
                     /gene="gcvT"
                     /locus_tag="PKH54_10755"
     CDS             complement(1168..2274)
                     /gene="gcvT"
                     /locus_tag="PKH54_10755"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOD00416.1"
                     /translation="MSDHPEARRTALYEEHVKAGGKLVPFAGFMLPIQYEGIAAEHHR
                     CRTAVGLFDVSHMGEIVFRGPEAAKVVNRLVTNNVVSVKPGRAVYTPCCRPDGGIVDD
                     MIFYKKSDTEWFVCVNASNKDKDYAWFVEQTRGECEVVDLSEQYSQIAVQGPNAPELL
                     ARVLGTEVATMKPFWFITKDYRGQEIIFATTGYTGEPGGEIYVPSAVAADLWRELMDK
                     GSDLGVGPIGLGARDSLRMEMKYCLYGNDIDDTTTPIEAGLQWTVKFDKGDFNGRDVH
                     LKQHEDGPARHLVQFVVEGKGVPRHNYAVYSGDRKIGHVTSGTMSPSLKIPIGIAYVE
                     PPFQTVGQTFEIDLMGLRRVQARVVEAPVYRKAK"
     gene            2513..>3322
                     /gene="folD"
                     /locus_tag="PKH54_10760"
     CDS             2513..>3322
                     /gene="folD"
                     /locus_tag="PKH54_10760"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOD00417.1"
                     /translation="MAKVIDGKAVAARVRADVARAVAELREKGVVPCLNVVLVGEDPA
                     SQVYVRMKGKACDEVGIRGVTHRLPVSTTEDELLALLKTLNADPEVDGILVQLPVPDH
                     IDPDKVTDALDPARDVDGFHPFSLGMLMSDRPGMEPCTPAGCIRLIDETGIDLKGRNA
                     VVIGRSLIVGKPMAMLLLRRHATVTVCHSRTRNLADVVRTAEVVVAAVGRPQMVKGDW
                     IREGAVVIDVGVNRLADGKLVGDVDFEAASSRASWITPVPGGVGPMTIAYLM"
BASE COUNT          611 a         1014 c          996 g          701 t
ORIGIN      
        1 gaccacggcg gcgacattgt caccgaccag tcgcctggcg gcatccatat cagtggtgct
       61 ggccggcgcc gccgggatca tcacgatttc gccggcgcca gtgtaagtcc ggagcacgtc
      121 gatgtactgc ggatgaacca gcgacgacac cgccagaatg tctcgcctgg tgtgcttctg
      181 ggccatcagg caggcctcgg tcatggctgt ggcgccttcg tacatgctgg cgtttgaaac
      241 atccattccc agtatctggg cgaccatcga ctggtattcg aactgcgcct ggagggtgcc
      301 ctgggacagt tccggctggt acggcgtgta gctggtaagg aactcgccgc gcccgacaag
      361 gtgatgaatc gccgccggaa cataatggtt gtaagcgccg ccgcccagaa aggaagggca
      421 ctgatccgcc gtcatgttca gggccgccag ttccttcatt cgggcaagga cgtcggcttc
      481 cgacatagac tccggaaggt tcatgcaacc cttaaccttg aactcggaag gaatcacggc
      541 gaaaagctcc tcgattgacg atacgccaat cactcgaagc atttcggcga tttcagcttc
      601 cgtgtgggga gtgaagtcca tccctcaaac tcctgctgaa gattggtatc cgccgcaacg
      661 gttccggcgg catctgtgga gcccggggct attcctcttc gaggcgtccc aggtattcct
      721 cgtagtcctc ggccgtcata agctcgtcga gctgttccat gtcggcgact tccacgcgga
      781 caagccagcc ttcctcgtaa ggatcgctgt tcatcacgct gggatcgtcc tcgagcaggt
      841 cgttcgactc cagcacaagg ccggtgagcg gggcgaagac ttcggtcgat gccttctggg
      901 aatcgatcat gccgatcggt tcgccctttt cgatttcgtc gccaaccctg ggggcttcaa
      961 cgcttacgat atcgcccagg tgatcctgcg cgtaatcggt gatgcctatt gaaacgatgt
     1021 tgtcctcttc gaccttcacc cactcgtgcg tgtcggtgaa cctgtactcc ttggggatcg
     1081 acatcagtaa cctccttgaa tccttcatca accggcctgg ccatcactcc cactgagtgt
     1141 cgggtccgaa gccggacagt tccaatttca cttcgccttc cgataaaccg gcgcctcgac
     1201 gacgcgggcc tggacgcggc gaaggcccat cagatcgatt tcaaacgtct ggccgaccgt
     1261 ctggaatggc ggctcgacgt atgcgattcc aattggaatc ttcaggctcg gcgacatcgt
     1321 gccgcttgtc acgtgtccga tcttccgatc gccggaatac accgcgtagt tgtggcgggg
     1381 aacccccttg ccttcaacca cgaactgcac caggtggcgt gcggggccat cctcgtgctg
     1441 cttcaggtgc acgtcgcggc cgttgaagtc gcccttgtcg aacttgaccg tccactgcaa
     1501 cccggcctcg atcggcgtcg tcgtgtcgtc gatgtcgttg ccgtaaaggc agtacttcat
     1561 ttccatgcgc aagctgtcgc gggcgccgag tccaatcggg ccgaccccca ggtcggatcc
     1621 cttgtccatc agttcgcgcc agagatcggc cgcgaccgcg ctgggaacgt agatttcgcc
     1681 gccgggctcg ccggtgtagc cggtcgtcgc gaagatgatt tcctggcccc ggtaatcctt
     1741 cgtgatgaac cagaacggct tcatcgtggc gacttccgtt cccagaaccc tggcaagcag
     1801 ttccggcgcg ttcggcccct gaaccgcgat ctgggaatac tgttccgaaa gatcaacgac
     1861 ttcgcattcg cccctggtct gctcgacgaa ccatgcgtaa tccttgtcct tgttggatgc
     1921 gttgacgcag acgaaccact cggtgtcgga cttcttgtag aagatcatgt cgtcaacgat
     1981 gccgccgtcc ggcctgcagc agggcgtata gaccgcccgg ccaggcttga ccgagacgac
     2041 gttgttcgtt accagcctgt tgacgacctt cgccgcctcc gggccacgga atacgatttc
     2101 acccatgtgg cttacgtcga aaaggccgac agccgtccgg cacctgtggt gctcggccgc
     2161 gatgccttcg tactggatgg gaagcatgaa accggcaaac ggcaccagct tgccaccggc
     2221 cttgacgtgt tcttcataaa gcgccgtgcg gcgcgcttcg ggatggtcgc tcacgtgaaa
     2281 acctccgggg ttgaccgggc accgaatata gcaacacagc acattggtca agcaaaattc
     2341 agcgggatgt cgaattgtcg tacccatcat cttcatccgc cggggggtgg cggacgcgat
     2401 tttgtacagg atttttttgc gcgcacaagc gcctccgcgc gcgattgacg cggcgttgcc
     2461 cgctgtatag aatccggcct ctcttcttgc gggggcagcc atgccagaat ccattgcaaa
     2521 ggtgatcgac ggaaaggccg tggcggcgcg cgttcgcgcc gatgtggcgc gggccgtcgc
     2581 cgaactgcgt gaaaagggtg tcgttccatg cctgaatgtc gttctggtgg gtgaggatcc
     2641 ggcttcccag gtttatgtga ggatgaaggg caaggcttgt gacgaggtcg gcatccgcgg
     2701 cgtcacgcat cgcctgccgg tctccacgac ggaggacgaa ctcctggccc ttcttaagac
     2761 attgaacgcc gaccccgagg ttgacggaat cctcgttcag ttgcccgtgc cggatcacat
     2821 agatcccgat aaggtcacgg acgccctgga tccggcaagg gatgtcgatg gattccatcc
     2881 gttcagcctc ggaatgctga tgtccgacag gccgggaatg gaaccgtgta ccccggccgg
     2941 ctgcatccgc ctgatcgatg aaacgggcat cgacctgaag ggcaggaacg cggtggtgat
     3001 cggccgcagc ctgattgtgg gcaagccgat ggcgatgctt cttctgcggc gtcacgccac
     3061 ggtgaccgtc tgtcattcca ggacaaggaa ccttgccgac gtcgtcagga cggccgaagt
     3121 tgtcgtggcc gccgtcggaa ggccccagat ggtcaagggc gactggattc gcgagggcgc
     3181 cgtcgtaatc gatgtcggcg tcaaccgcct ggcggacggc aaactggtcg gcgacgtcga
     3241 tttcgaggca gcttcctcaa gggcttcatg gatcaccccg gttcccggcg gtgtcggtcc
     3301 catgacaatc gcctatctga tg
//
LOCUS       DAOPPA010000373         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31406_length_3307_cov_4.217405, whole genome shotgun sequence.
ACCESSION   DAOPPA010000373 DAOPPA010000000
VERSION     DAOPPA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31406_length_3307_cov_4.217405"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKH54_10765"
     CDS             <1..665
                     /locus_tag="PKH54_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD00418.1"
                     /translation="DGREIWRAFLGGVGPGSLPGSNGSETSPAVHAGLVYAATYDGAL
                     FCVDAESGRVVWKARTGDDTDASPVISGDNVIVASESRSSDVICFDRMTGSRKWRFNG
                     CRGGYWATPAVRDGRVFVGGGAGGGMFCLDERDGSLIWHTRLTPWIWSSPCVVDGRVI
                     FGAHDGLLRMLDAKDGRLVAEVDLGGRILSTPCIVNGTIFVGTGEGRFFCIDTARRET
                     SG"
     gene            complement(684..2609)
                     /locus_tag="PKH54_10770"
     CDS             complement(684..2609)
                     /locus_tag="PKH54_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOD00419.1"
                     /translation="MERNFICVFFLGCLAAATGCGQDANLSVYDTASDAVQDSALPAD
                     ADHDLDDAVSDDRIWYIDTTEGDSAGIPDGGTGTDLSIEPDQSVLPDSSEDHDEGTPG
                     DTKVPDCGTDGVTCIFLNHDSISVSGAGAVADGAAVTVVSAGVFLISGTLDDGSITVD
                     TTDSGLVRLLLNGIHASCSTSSPLFIMDAGDVEIHLVAGTTSTLTDAVTYVYADAGQD
                     EPNATIFSKSDLDITGDGTLIVNGRYNDAINVKDGLDILGGTIVASAVDDGIRGKDNV
                     TILGGSVTVTAGNNGIKSDNDTDEGRGFIEVASSHVNVTSGGDALTAQRAISIRGGDI
                     KLVSGGGAGASGSLDVSAKGIKGLESVVISGGNIEIDSADDGVHSDNAILVTGGSCLV
                     ASGDDAFHAEETLEITGGSINITRSYEGLESKLITIDDGTIHIVSSDDGINVAGGDGS
                     GGWTPGGGGSSAAYILTINGGLVWMNAGGDGLDSNGNMVINGGTVLVNGPTDNGNGPI
                     DIGDGSGYYFRMNGGLVVAAGSSGMAVGNSTSSTQYGVLVNLTAAQTAGTIFHLRASS
                     GTEMLTFKPAKKYQSVFFSSPSLASGSYDVYLNGTSSGVLNDNLITGGVYTPGTKYTT
                     FSATGVATRVGSSGPPH"
     gene            2853..>3307
                     /locus_tag="PKH54_10775"
     CDS             2853..>3307
                     /locus_tag="PKH54_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00420.1"
                     /translation="MTETAGHLFERHLHRALRLVALALVPTVASCYFPPDPLPITLDK
                     ARTVISQNVVSQASALDTGLKFMEGMALFKRVGAYLGGGMGCSMPVEGVDLPAEPDCE
                     FEMGDDHFELGIEESARRLIEMINEFVLVESQIARFSDTSIEIRLLPDV"
BASE COUNT          640 a          946 c          993 g          728 t
ORIGIN      
        1 aggatggacg cgagatctgg cgggcgttcc ttggtggcgt cggccccgga tccctgccgg
       61 gatccaatgg ttccgagact tccccggctg ttcacgccgg cctggtttat gcggcgacgt
      121 acgacggtgc tttgttctgc gtcgatgccg agtcgggcag ggtggtctgg aaggcccgga
      181 ccggcgatga taccgacgca agccccgtca taagcggtga caacgtgatc gttgcgagtg
      241 aaagccgatc aagcgacgta atctgcttcg atcgcatgac aggaagtcgg aagtggcgct
      301 tcaacggctg tcggggcggc tactgggcaa cgccggcggt tcgggatgga agggttttcg
      361 tcggcggtgg cgctggtggc gggatgttct gtcttgatga acgtgacggt tcactgatat
      421 ggcacacgcg gcttacgccc tggatctggt cgtcaccatg cgtggttgat ggccgggtga
      481 tttttggcgc gcatgatggc cttctgagaa tgcttgacgc gaaagatggc aggcttgttg
      541 ccgaggtgga tctgggcggc agaatccttt caaccccctg catagtcaat ggaacaatct
      601 tcgtgggaac gggtgaaggc cgtttcttct gtattgatac cgcgaggcgg gaaaccagcg
      661 gatgaagccg cgttcgcccg atttcaatgc ggcggcccac ttgagccgac cctggtggcc
      721 acgcctgtgg ccgaaaacgt cgtgtacttc gtgccgggcg tatatacgcc cccggtgata
      781 agattgtcat tcagcacgcc ggacgaagtt ccattcagat agacgtcgta gcttcccgac
      841 gcaagcgagg gactgctgaa gaacaccgac tgatacttct tggccggctt gaatgtgagc
      901 atctcggttc ccgaagaggc gcgaaggtgg aatatggtcc ccgcggtctg tgcggccgtc
      961 agattcacaa gaacgccgta ctgcgtcgag gaagtgctgt tgccaacagc catgccgctg
     1021 cttcccgccg cgacaaccag gcctccgttc atccggaagt agtaaccaga gccgtcaccg
     1081 atatcgatcg gcccatttcc gttgtcggtc gggccgttga caagaacggt tccaccgttg
     1141 attaccatgt tgccgttcga atcgagtccg tcaccaccgg cattcatcca taccaggcca
     1201 ccgttaatcg taagaatgta tgcggccgag gagccgcccc cgccgggggt ccatccgccg
     1261 gaaccgtcgc ccccggcaac gttgatgccg tcgtcggaag aaacgatatg aatcgttccg
     1321 tcatcaatcg ttatcagctt gctttccagg ccctcgtaag accttgtgat gttgattgag
     1381 ccaccggtta tctccagggt ttcctcggca tggaacgcgt cgtccccgga tgcgaccaga
     1441 catgaccccc cggttacaag gatcgcgttg tcgctgtgga cgccgtcatc ggccgagtcg
     1501 atttcgatgt tcccacccga gataacgaca ctctccagcc ccttgatccc cttggcgctt
     1561 acatcaaggg aacctgacgc gcccgccccc ccgccgctga cgagcttgat gtcaccgcca
     1621 cggatggaga tcgccctttg cgcggtcagc gcatcgccgc ccgatgtaac atttacgtgg
     1681 ctggaggcga cttcaatgaa gccgcgacct tcatcggtat cgttgtccga cttgataccg
     1741 ttattaccgg ccgtgacagt gaccgagcca ccaagaattg tgacgttgtc ctttcccctg
     1801 atgccgtcat caacggcgct tgccactatg gtgccgccga ggatgtccag gccgtccttt
     1861 acattgatgg cgtcgttgta acgcccattg acaatcaggg taccgtcgcc ggtgatatcc
     1921 aggtcagatt ttgaaaagat ggttgcgttt ggctcgtcct gcccagcatc ggcatacacg
     1981 tacgtgacgg catccgtaag tgtgctggtg gttcccgcta caagatgaat ttccacatcc
     2041 ccggcatcca tgatgaagag tggcgatgac gtgctgcagg acgcatggat cccgttaagc
     2101 agcaaacgta caagcccact gtcggtcgta tcgacggtga tgcttccgtc gtcaagggtt
     2161 ccggaaataa ggaatacgcc cgccgacacg actgtgaccg cagccccgtc agcgacggcc
     2221 ccggcaccgc tcacgctgat gctgtcatga ttcaggaaga tgcatgtaac gccgtcagtc
     2281 ccgcagtcgg gaacctttgt gtcgcccggc gtgccctcgt catggtcttc ggacgagtca
     2341 ggaagaaccg actgatccgg ctcgatggac aaatcggtgc ccgtgccgcc gtccgggatg
     2401 ccagccgagt cgccttcggt ggtgtcgatg taccatatgc ggtcatcgga aacggcgtcg
     2461 tcgagatcgt gatccgcatc ggcaggcagg gcggaatcct gcacggcgtc agacgcggta
     2521 tcgtaaacag acaggttggc atcttgcccg cagccagtcg cggccgccag acagcccaga
     2581 aaaaacacgc agatgaaatt tctttccacg ttgcccccac tttgccaatt cgacccgtag
     2641 tccgtcagtc atgccggccg caaacggtcc ttacccctta tcggagtggt ccgacatcgg
     2701 gccgcaaacg tggcgatgat aagacccggg tgttaagcaa tccttaagta catggcggta
     2761 ttggaattat tcagtccggt tcgcaacaat gtgggctcgt gatgttcgtt tcggctttgg
     2821 tgccgcccga tggcacccca gggaggtcgg gaatgactga aaccgcgggt cacttgttcg
     2881 aaaggcattt gcaccgggca ttacggctgg tggccctggc cctggttccg acagtggctt
     2941 cctgctattt tcctccggac ccgctgccaa tcacgcttga taaggccagg actgtcatct
     3001 cgcagaatgt cgtttcgcag gcatcggcac tggataccgg gcttaagttc atggaaggaa
     3061 tggcgttgtt caagcgggtt ggcgcgtacc tcggcggcgg gatgggttgc tcgatgccgg
     3121 tggaaggcgt tgatcttccg gctgaaccgg actgcgaatt cgagatgggc gatgatcact
     3181 ttgaattggg cattgaggag tcggcacgtc ggctgattga gatgatcaat gagttcgtcc
     3241 ttgtggaatc ccagattgcc agattttcgg acaccagtat cgagattcgc cttctgcccg
     3301 acgtatt
//
LOCUS       DAOPPA010000374         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31576_length_3293_cov_2.176961, whole genome shotgun sequence.
ACCESSION   DAOPPA010000374 DAOPPA010000000
VERSION     DAOPPA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31576_length_3293_cov_2.176961"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PKH54_10780"
     CDS             <1..1221
                     /locus_tag="PKH54_10780"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00421.1"
                     /translation="KPVRLKLDPSFISRRLGAAVSSDTTLGILTRLGFVVDIRPDGNF
                     MVTVPSWRATKDVSIAEDLLEEVGRFVGYDNIVPVQPKAVVSLVPRVERRAFIYRSKH
                     ILAGDCGLDEIERYSFDSQDILKRIGFSPADPVRIKNPISADFATLRTQLAPNMLDAM
                     ELNSKNFNEFQMFEVGRVFSATHDKDGIPTQGYHIGILAFNRLARKPRQLEDLFRQMR
                     GVVERFTQMHGFKAVTMSEPLSGIPNQPAFPWLHPSASLAIRIADRFVGYATCVHPAV
                     MRGLDVLGGAVYVDFDLEALLDCPRQGAKFCSIPRFPSSQIDLSLIVPERTFASVLEG
                     AIVQGGGPFLAAVELVDVYVGKPIPDGFKSMTYRIVFQAADRTLSDDEIKSAIEVITA
                     GARAAGATLWGETR"
     gene            1226..2356
                     /locus_tag="PKH54_10785"
     CDS             1226..2356
                     /locus_tag="PKH54_10785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD00422.1"
                     /translation="MKAPGILFVTKPLVAPWADSGRLLPYLVAKNLADLRISVMVPSG
                     QPLDLPNVVSEEVYASGHAFSVPGGDKLRLMARLMGRATPPVVHFFFSPNRPTSMAAR
                     LVARRHRGVHMIQTIMSLPMKDRDLVEGIFADTVVVWSEHARRRVADIVAARGLSSDV
                     VHIRPGIEPIEPIGEAHRRFLRTGFGLPPDGRIILYAGDLEFSTGATVTAAAVRMLAD
                     RMRAVFVFACRPKTPAASRVLKRIEADLALEASAGRVRFMGQVGNFRDLLSCVDCQVL
                     PVETTYAKTDIPLVVLEGLSAGVPAIVGTGTPMDELVACGAAAGVMPGDPRALADAVE
                     GVFGSGGNCDTLAKAGRRYVLANHGVQAMAQAHAVMYRRLAG"
     gene            2366..3070
                     /locus_tag="PKH54_10790"
     CDS             2366..3070
                     /locus_tag="PKH54_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD00423.1"
                     /translation="MENQSYYDVMSKGYEKERHHGYHAWLDRAEIDTISDLIRGRDVL
                     EVGCGTGLMMTRIAPIARHVTGVDLSAGMLEKARERGLNVMQADATKLPFADASFDAA
                     VSFKVLAHIENIQAAMSEMCRVVRPGGIVAAEFYNRHSIRSLIKFTKKPSEIGAGVVD
                     TDVFTRYDSISQAESYFPQGMEIIRVRGIRTVIPAAVFMKIPVLDRALGFQDAIVSRT
                     PFGRLGGFVVVVARKA"
     gene            complement(3077..>3293)
                     /locus_tag="PKH54_10795"
     CDS             complement(3077..>3293)
                     /locus_tag="PKH54_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOD00424.1"
                     /translation="ARLGGLLLAMFLVPTTLIFHAFWNLEGQERYMQMLMFNKNLAII
                     GGLLYVAVFGAGKFSLDNLVCPLRKKK"
BASE COUNT          595 a          948 c         1038 g          712 t
ORIGIN      
        1 aaacctgtcc ggttgaagct ggatccctca ttcatctcgc gccgtctcgg ggcggcggtc
       61 agctccgaca cgaccctggg catccttacc aggcttgggt tcgtcgtcga tatcaggccc
      121 gatggaaatt tcatggtcac cgttccgtca tggagggcga ccaaggacgt ttcgatcgcc
      181 gaggacctgc ttgaagaggt cggccgtttc gtcgggtacg acaacatcgt gccggttcag
      241 ccgaaggccg tggtaagcct ggttccacgg gtcgagcgcc gcgcgttcat ataccgcagc
      301 aagcacattc ttgccggtga ctgcgggctg gacgagatcg agcgctattc gttcgacagc
      361 caggacatct tgaaaaggat cggtttttcc ccggccgacc cggtgcgaat caagaacccg
      421 atttctgccg atttcgccac ccttcgtacc caactggcgc cgaatatgct ggatgcgatg
      481 gagctgaatt ccaagaactt caacgaattc cagatgtttg aagttggtcg cgtcttttct
      541 gcgacccacg acaaggacgg cattccgacc cagggttacc atatcggtat cctggcattc
      601 aatcgactgg ccagaaagcc gcgtcagctc gaggaccttt tccgccagat gcgcggcgtc
      661 gtcgagcggt tcacgcagat gcacggattc aaggcggtga ccatgtccga gccgttgtcc
      721 gggattccga atcagccggc cttcccgtgg cttcatccgt cggcatcgct tgcgataagg
      781 attgcggaca ggtttgtcgg gtatgcgaca tgcgtgcacc cggccgtgat gcgtggcctt
      841 gacgttcttg gcggcgcggt ttacgtggat ttcgacctcg aggccctgct tgattgcccg
      901 cgtcagggcg cgaagttttg cagcatcccg cgcttccctt caagccagat tgatctttcc
      961 ctgattgtcc cggaacggac gttcgcgtcg gtgctggaag gcgccatcgt ccagggcggc
     1021 gggccattcc tggcggccgt tgaactggtt gacgtctatg ttggaaagcc gattccggac
     1081 ggtttcaaga gcatgacgta tcgcatagtg ttccaggccg ctgatcgcac gctttcggac
     1141 gacgaaatca agtcggccat cgaggtcatc acggccggcg ccagggcggc gggggcgact
     1201 ttgtggggcg aaacgaggtg acttcttgaa ggcacccgga atcctctttg tcaccaagcc
     1261 tcttgtggcc ccatgggccg attcaggccg cctgcttccc tatcttgtcg cgaagaacct
     1321 tgctgacttg cgaatttcgg tcatggtgcc ttccgggcag ccgcttgacc tgcccaacgt
     1381 cgtctccgag gaggtatatg cctccggcca tgcattttcg gtgcctggcg gtgacaagct
     1441 tcggctgatg gcccgtttga tgggtcgcgc gacgcccccg gtagtccatt tcttcttttc
     1501 gcccaaccgt ccgacgtcga tggcggcccg tcttgttgcc cgcaggcatc gcggggtgca
     1561 catgatccag acgatcatga gccttccgat gaaggatcgt gatctggtcg agggaatttt
     1621 tgccgatacg gtcgtcgtct ggtccgaaca cgcccgtcgt cgggtggccg atatcgtggc
     1681 agcccgcggt ctgtcgtccg atgtcgtcca catccgcccg ggaatcgagc cgattgagcc
     1741 catcggggaa gcgcaccgtc ggttccttcg caccgggttc ggcctgccgc ccgacggacg
     1801 gataatcctt tatgccgggg atctggaatt ctcaaccggc gcgaccgtga ccgccgccgc
     1861 ggtaaggatg cttgcggatc gcatgcgggc cgtcttcgtt tttgcgtgcc gcccgaagac
     1921 gccggccgcg tcaagggtgc ttaagaggat tgaagccgac ctggctctcg aggcatcggc
     1981 gggcagggtc cgcttcatgg ggcaggtcgg caatttcagg gatctgcttt cgtgtgtcga
     2041 ttgccaggtt cttccggttg aaacgacata cgcgaagacc gacattccgc tggtcgtgct
     2101 tgaaggactt tcggcggggg tgccggcaat cgtgggcacc gggacgccca tggacgagct
     2161 tgtggcctgc ggggcggcgg ccggggtgat gccgggggat ccacgggccc tggccgatgc
     2221 cgtggaaggc gttttcggat ccggcggcaa ctgcgatacc cttgcaaagg cgggacggcg
     2281 atacgtgctt gccaatcatg gtgtccaggc catggcgcag gcccacgcgg tgatgtacag
     2341 aaggctggcg ggctgacggg ggcaaatgga aaatcaaagt tattacgacg tgatgtcaaa
     2401 gggttacgaa aaggagcggc accacggata tcacgcgtgg ctggatcgtg ccgaaattga
     2461 cacaatctcg gacctgattc gcggtcgcga cgtgctggaa gtcggttgcg gaaccgggct
     2521 gatgatgacg cgcatcgcgc cgattgcacg tcacgtgacc ggtgttgacc tgtcggcggg
     2581 aatgctggaa aaggcacgcg aacgcggact gaacgtcatg caggccgatg cgacgaagtt
     2641 gccttttgcc gacgcatcgt tcgacgcggc ggtcagtttc aaggtgcttg cccacattga
     2701 aaacatccag gccgcgatgt ccgagatgtg ccgggtggtg cgcccaggtg gaatcgtggc
     2761 ggccgagttt tacaaccgtc acagcatcag gtcgcttatc aagttcacca agaagccgtc
     2821 cgagatcggg gcgggtgttg tcgataccga cgtcttcacc cgttacgatt cgatttcaca
     2881 ggcggaatcg tactttccgc aaggcatgga aatcatccgc gtccgtggca ttcgaacggt
     2941 tattcccgcg gccgttttca tgaagattcc tgttctggat cgggcacttg ggtttcagga
     3001 tgccatcgtc agccgcacgc cgttcggacg tctgggcggc tttgtcgtcg tcgtcgcccg
     3061 caaggcatga cgacggtcac tttttcttcc gcaacgggca gaccaggttg tccaggctga
     3121 attttcccgc accgaatacc gcgacgtaca gcaggccgcc gataatcgcc aggttcttgt
     3181 tgaacatcag catctgcatg taccgttcct ggccttcaag attccagaag gcgtgaaaaa
     3241 tcaaggtggt gggaaccagg aacatggcca ggagcagtcc gcccagacgg gcc
//
LOCUS       DAOPPA010000375         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31599_length_3291_cov_3.329419, whole genome shotgun sequence.
ACCESSION   DAOPPA010000375 DAOPPA010000000
VERSION     DAOPPA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31599_length_3291_cov_3.329419"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKH54_10800"
     CDS             <1..373
                     /locus_tag="PKH54_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD00425.1"
                     /translation="PTAASGLVPDNPQVPLNVILDRQEKLYLDGTETTLEKIGTEIDR
                     RRESGGDPQAVVSADKDLSWGRITRVIDYLKLKGIGNLAVNVEEQTIDPSATGAGGAP
                     APGAAGADGTAGAAGTGATGG"
     gene            411..1289
                     /locus_tag="PKH54_10805"
     CDS             411..1289
                     /locus_tag="PKH54_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD00426.1"
                     /translation="MGGGSKSREMPRSLILGVSVGLSVLMHVGAYLAAPDERPAPPPR
                     PKKVAVRPVPIQVRQDLIRKLEEPSPEPKPIEKTPEPPKPIPKKAEPPKPKQETPKPP
                     PKQEPKPKPPKPVEPPREVPPPPAPPAEPAKPKPFVLKNVALKGSVAVQTGDDSNLFG
                     DPTVSAKGFVKGVDAPSVPGNDGTGGEGGTGGQGGPAKEKVVITQPKALNDVKGQYPQ
                     AFRDLRRVVRVELMLTIGSDGAVKSVKVRKGAEQEFNDAAIRAVNQLKFEPATRNGVP
                     IAFNLKWTVVFIPEGT"
     gene            1286..2503
                     /locus_tag="PKH54_10810"
     CDS             1286..2503
                     /locus_tag="PKH54_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00427.1"
                     /translation="MITRLAERTRTAVALTGAFAVVVAAGCMPDYNPSNFIEKYRVIG
                     VMAEPPAVEFTPEYQGTVRLELVEAFPLPPTGDLVGPEIEGIEWSVCLISAGAAANFA
                     CTLPEIPVTDVSDSGRIATFDGALIALGLEFVRDQVEVLMDGIRQAVAFTDQCNKDLI
                     VEYDDCLKDGDLETCNGNAFEGFRKCVFQNGLNPVFKVRVTVRDMLLDDSDLPVFDDD
                     GKPVMRQRVLETFKTVTFGGLKEGQKPNRNPQFSVRLGAKPVDDLFDMMHPEGLVATT
                     LGNHEQPEILACPGQEIVFQATVPPDSIDRVTGDDGVETDEYWVISWYANNGRFKKIR
                     IGSVTAEGVDLDLTNTLKFKEEEPVRRTQVTLVMRDELFGLNFLRFFVGAQTRDKCPA
                     PGTLDSLKKGDKP"
     gene            2500..>3291
                     /locus_tag="PKH54_10815"
     CDS             2500..>3291
                     /locus_tag="PKH54_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD00428.1"
                     /translation="MKRLALASFGALVLALAPMSAISMTTADAPTVEGAVGGDPVDEG
                     PAVEGPADEGPIEEGPVQEGPVQEGPADKDDGEGPVQEGSADDGSDDEGPVDEGPVEE
                     GAQGPAADAGDVLQEVTPPELLEFVQAVLPPELDIPDGQYVVVLTLTIDENGAVTEVS
                     AADPPRPELVAPAIEAAKKFRFKPATYQDKAVLVQVQYEYQFEVRQKIREVVRVFLTL
                     ERGTRDPISEVSGFIEETGATFTSIDGRMEISNLQPGKYTLYIPAG"
BASE COUNT          696 a          942 c         1103 g          550 t
ORIGIN      
        1 gccgacagcg gcgagcgggc ttgtcccgga caatccgcag gttcccctga acgtcatact
       61 ggacaggcag gaaaagctgt atctggacgg cactgaaacg accctggaaa agatcgggac
      121 ggaaatcgac aggcgacgcg agtccggcgg tgatccccag gcggtcgtgt cagctgacaa
      181 ggacttgagc tggggacgta ttacgcgggt catcgattac ctgaaactga aaggcatcgg
      241 caaccttgcc gtcaacgtcg aggaacagac gatcgatccg tcggccacgg gtgccggggg
      301 cgcgcccgcg cctggtgcgg ctggcgcgga tggcaccgcg ggtgccgccg gaacgggcgc
      361 gacgggcggg tgacactggt ttgcggcatg tccttcgggg gacttcagac gtgggcggcg
      421 gttcaaaatc acgcgaaatg ccaaggtcgc tgatcctggg tgtcagcgtg ggcctgtccg
      481 tgctgatgca cgtgggggca tatctggccg cccctgacga acggcccgcc ccgccgccgc
      541 ggccgaagaa ggttgccgtg cgcccggtcc cgatccaggt caggcaggat ctgatcagga
      601 agcttgagga gccctcgccg gaaccgaaac cgatcgagaa gacccccgag ccccccaagc
      661 caatcccaaa gaaggccgag cccccgaaac cgaaacagga aacaccgaag ccgccgccga
      721 agcaggagcc gaagccgaag ccgccaaaac cggtcgaacc gcccagggaa gtcccgccgc
      781 cgcccgcccc gccggcggaa ccggccaagc cgaagccttt cgtgctgaag aatgtggccc
      841 tgaagggcag cgtcgcggtt cagaccgggg atgacagcaa cctgttcggc gacccgaccg
      901 tcagtgccaa aggtttcgta aagggcgtcg atgcgccttc ggttcccggg aacgacggca
      961 ccggcggtga aggggggact ggcggccagg gcgggccggc aaaggagaag gtcgtcatca
     1021 cccagcccaa ggcgcttaac gacgtcaagg ggcagtaccc ccaggccttc agggatctgc
     1081 gacgggtggt ccgcgtggaa ctgatgctga ctatcggctc ggatggcgcg gtcaagtcgg
     1141 tgaaagtgcg aaaaggtgcc gagcaggaat tcaacgacgc ggcgattcgc gcggtcaacc
     1201 agttgaagtt cgagccggcg accaggaacg gcgtcccgat tgcattcaac ctgaagtgga
     1261 cggtcgtctt cattccggag gggacatgat aaccaggctg gctgaaagga caaggacggc
     1321 cgtggctttg accggggctt tcgcggtcgt ggtggccgcg ggatgcatgc cggattacaa
     1381 cccgtccaac ttcatcgaga agtaccgggt aatcggggtc atggccgagc cgcccgcggt
     1441 cgagttcacg ccggaatacc aggggaccgt gagacttgag ctggtcgagg cgttcccatt
     1501 gccgccaacc ggcgatctgg tcgggccgga aattgaaggc atcgaatggt ccgtctgcct
     1561 gatttcggcc ggtgccgcgg caaatttcgc gtgcacgctg cctgaaatcc cggttaccga
     1621 cgtttcagac tcgggccgga tcgcgacatt cgacggcgcg ctgattgccc ttggcctgga
     1681 attcgtccgg gatcaggtgg aagtcctgat ggacggaata cggcaggcgg tcgccttcac
     1741 ggaccagtgc aacaaggatc ttattgtcga atacgatgat tgcctgaagg acggggatct
     1801 tgaaacctgc aacgggaacg cattcgaagg tttcagaaaa tgcgtcttcc agaacggcct
     1861 gaatccagtc ttcaaggttc gcgtaacggt cagggatatg ctgctggacg actcggacct
     1921 gccggtattt gacgacgacg gcaagccggt gatgcgccag cgggtgctgg aaacattcaa
     1981 gacagtaacc ttcggcggat tgaaggaagg acagaagccg aacaggaacc cgcagttttc
     2041 cgtccggctg ggcgccaagc ccgtcgatga ccttttcgac atgatgcacc cggaaggtct
     2101 tgtggcgacg acgcttggaa atcatgaaca gccggaaatc ctggcgtgtc cggggcagga
     2161 aattgtcttc caggcaacgg ttccgccgga ttcgattgat cgcgttaccg gggatgacgg
     2221 tgtcgagacc gacgaatact gggtcatttc ctggtatgcc aacaacggaa ggttcaagaa
     2281 gattcgaatc gggtcggtca ccgcggaagg tgtcgatctg gatctgacca acacattgaa
     2341 attcaaggag gaagagccgg tgcggcgcac tcaggtcacc ctggtgatgc gcgacgagct
     2401 cttcggcctg aactttttga ggttctttgt aggggcccag acccgggaca aatgccccgc
     2461 gccgggaacc ctcgacagcc tgaagaaggg ggacaagcca tgaaaagact tgcccttgca
     2521 tcgttcggcg cgcttgttct ggcgcttgcc ccaatgtcgg ccatatcgat gacgactgcc
     2581 gacgccccga ccgtggaagg cgcggtgggc ggggatcccg ttgacgaagg tccggcagtt
     2641 gaaggcccgg ccgacgaggg cccgatcgag gaaggtcccg tccaggaagg tcccgtccag
     2701 gaaggtccgg cggacaagga tgacggcgag gggccggttc aggaaggttc ggcggacgac
     2761 ggttcggacg acgaaggacc ggttgatgag ggccctgtcg aggaaggcgc tcagggcccg
     2821 gccgcggacg caggcgacgt ccttcaggaa gtcaccccgc cagaactgct ggagttcgtg
     2881 caggccgtgc ttccgccgga gttggacatc cccgacggcc agtacgttgt cgtgcttact
     2941 ttgacgatcg atgaaaacgg ggcggtcacg gaagtgtcgg cggccgatcc gccgcgtccc
     3001 gagcttgtcg cgccggcaat cgaggccgcg aagaaattcc gtttcaagcc ggccacgtat
     3061 caggacaagg cggttcttgt ccaggtgcag tacgaatacc agtttgaagt caggcagaag
     3121 atccgcgaag tcgtaagggt gttcctgacg ctggaacgcg gcaccaggga tccgatttcc
     3181 gaagtgtccg gattcatcga ggaaaccggc gcaacattca catccatcga cggccggatg
     3241 gagatttcaa accttcagcc aggcaagtac accctttaca ttccggccgg c
//
LOCUS       DAOPPA010000376         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31656_length_3286_cov_15.742495, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000376 DAOPPA010000000
VERSION     DAOPPA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31656_length_3286_cov_15.742495"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PKH54_10820"
     CDS             <1..1145
                     /locus_tag="PKH54_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00429.1"
                     /translation="GEVRYVPTPLSLIGKQFILVDEVSRAHPALQNKWLEIIRSRRVM
                     GLDLPELRTVFGAMNPAGLAGTVALDEALAGRFTFHISVPEISEMSQVDRHAVIQAAD
                     SNPLNPLDKSSRLRWVVRAVSDRQPVVTERHGHRIVRYVDALSTYLAGKDWKLDGRRQ
                     GMILRGLVAYVAVREVFTGQDLDAGLLGSVIRQGLDTLFPFRAMGRELPRVTIDGAHQ
                     YALAAMAGNARQLPPSDLLAAAMQLVAGDVVVDDDQLSLLVSRTLQAFDHPSGNDDGI
                     RAGAAMIVMATSVDAVRRLPLEARQRLIVAFSDTLELEPEDVQEFIDETVKQKSDELG
                     PELFNVVQRVSYNLTKRANSGRFTAGTDFGDLASSMARLVYEGGVA"
     gene            1142..2272
                     /locus_tag="PKH54_10825"
     CDS             1142..2272
                     /locus_tag="PKH54_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00430.1"
                     /translation="MIFKALKQSNTGGDVKLMILKDVRIPLKTVIDSGSFPIEKAELQ
                     MDSLSVSVRARSGVFRNVPMRLDTAPKEDFGKPGILVAKNTALATEYLVRHWVCGGDP
                     VIDAAVTAARQARDAGLSFLHIVQQFANPERQRHGWHDDNPECDGFIWPQEQFDFEFS
                     RVGGKGSRTYTVDRPRGESAMAAVLVPRSREFGGHVIRGWDVLTFGGESDNRIVIAST
                     PEGWQSGPFRRIDQETLARFESRGRMVAIGQADLSAWQNWVVPAIVRVPAAGIRPGAE
                     QDPDIVREFPVARLADARLPLAFTRDVQVIADPAAALDILCRASRGYVLAGLGGDVLP
                     AAFHRLAGRWLAVCVPALWVDGSRRCAGQVPDALWLSPAGAA"
     gene            2284..>3286
                     /locus_tag="PKH54_10830"
     CDS             2284..>3286
                     /locus_tag="PKH54_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00431.1"
                     /translation="MSGPVTTIDLEKRIWRAVELSSDPGALAAALHELLPRCTFEVRD
                     DVPTARIKNGSGGIVIEFGREFLARELGDDRDFLFVLMHEIYHHVLGHLRVSRGDRVS
                     RMYRGLSNIAADMLVNRTVCQRFFPDGVPLLDRMYPVDSLPGALLRNPGFSFETPAHQ
                     DARRRLIQNYEIGLAKMGANPGLCMRVFSVYRLAWGRGAPYETVLERLLALFRSIDPS
                     SFPQVILLGDHEPGAGGLGGIFSMDGTGDKGDPFTGGGEDREEIVRVKKTEHNQGVAA
                     ALRRALEVQANRWRMHDPIACPGVVCVPGRRDAAFLGAGMYPVFYQANKTEFEPEQLA
                     "
BASE COUNT          566 a         1003 c         1092 g          625 t
ORIGIN      
        1 gcggcgaagt ccggtacgtc ccgacgccgc tgtccctgat cggcaagcag ttcatccttg
       61 ttgacgaagt cagccgcgcc cacccggccc tgcagaacaa gtggctggaa atcatccggt
      121 cgcgtcgtgt catgggactg gacctgccag aactccgcac cgtgttcggc gcaatgaatc
      181 cggccgggct tgccggaacc gtcgcgcttg acgaagcact tgcgggcagg ttcacgttcc
      241 atatcagcgt gccggaaatc agcgaaatgt cgcaggtcga tcggcacgcc gtcatccagg
      301 ccgccgacag caacccgctg aacccgctgg ataaatcgtc gcggcttcgc tgggtcgttc
      361 gcgcggtttc cgaccgccag cccgttgtca ccgaacgtca cggccaccgc atcgtcaggt
      421 acgtcgacgc gctgtccacc tatcttgccg gcaaggactg gaaactggat ggccgccgtc
      481 agggcatgat cttgcgcggc ctggtcgctt acgtcgccgt gcgcgaagta ttcacgggac
      541 aagatctgga cgcgggcctc ctgggcagcg tgatccgcca ggggctcgac acgctttttc
      601 cgtttcgggc gatggggcgc gaattgcccc gggtgacgat cgacggggcg caccagtatg
      661 cgctggcggc catggctggg aacgcccgtc agcttccccc gtccgacctg ctggccgccg
      721 cgatgcaact ggtcgcgggc gacgtggtgg tggacgacga ccagctttca ctgctggttt
      781 cccggacgct gcaggccttc gaccatccct ccggcaacga tgacggaatt cgcgctggcg
      841 cggcgatgat cgtgatggcc acttccgtgg atgcggtgcg ccgtcttccg ctggaggcgc
      901 gccagcgcct gattgtggcg ttttctgaca cccttgaact ggaacccgaa gacgtccagg
      961 aattcattga cgaaacggtc aagcagaaat ccgatgaact ggggcccgag ttgttcaacg
     1021 tcgtgcagcg cgtttcttac aaccttacaa agcgcgcgaa ttcggggcgc tttaccgccg
     1081 gaactgactt cggggatctg gcttcttcca tggccaggct tgtttatgaa gggggtgtcg
     1141 catgattttc aaggcgttga aacaatccaa taccggcggc gacgtgaagc tgatgatcct
     1201 gaaggacgtc cgtatcccgt tgaaaaccgt cattgattcg ggcagttttc cgatcgaaaa
     1261 ggccgaactg cagatggact cccttagcgt ttcggtcagg gccaggtcgg gcgtttttcg
     1321 caacgtcccg atgcgtcttg atacggcgcc gaaagaggac ttcggcaagc ccgggatact
     1381 ggtcgcgaaa aacacggcgc tggcgaccga atacctggtc cgccactggg tctgcggcgg
     1441 cgacccggtc atcgacgcgg cagtgacggc cgcccgccag gctcgtgacg ccgggctgtc
     1501 gttcctgcac atcgtccagc agttcgcgaa cccggaacgc cagcggcacg gctggcacga
     1561 cgacaatcct gaatgtgacg gcttcatctg gccgcaggag cagtttgatt tcgaattcag
     1621 cagggtcggg ggcaagggct cgcggacata cacggtggac cgtcccaggg gtgaaagcgc
     1681 catggcggcg gtgctggtgc cgcgcagccg ggaattcggc ggtcatgtga tcagggggtg
     1741 ggatgtcctg actttcgggg gtgaaagcga taacaggatt gtgatcgcat cgaccccgga
     1801 aggatggcag agcggtccct tccgccgtat cgaccaggaa acccttgccc ggttcgaaag
     1861 ccgcgggcgc atggttgcca tcgggcaggc ggacctgagc gcctggcaga actgggtcgt
     1921 gcccgccatc gtaagggtgc ccgcagcagg tatccgcccg ggggcggaac aggatccgga
     1981 catcgtgcgc gaattccctg tggcgcgtct tgccgatgcc cggctgccgc tggcattcac
     2041 gcgtgacgtg caggtcattg ctgacccggc agcggcgctg gatatcctgt gtcgcgcatc
     2101 gcgtggatac gtgctggcgg gacttggcgg cgacgtcttg cccgcggctt tccatcgtct
     2161 tgccggccgc tggctggccg tctgcgtgcc ggctttgtgg gttgacgggt cgcgccgatg
     2221 cgctggccag gtcccggatg ccctgtggct cagccctgcc ggcgcggcct gagggggtga
     2281 aacatgtctg gaccggtcac gacgatcgac ctggaaaaac gaatctggcg cgcagtcgaa
     2341 ttgtcttcgg accccggggc gttggcggcg gcgcttcatg aattgctgcc gcggtgcact
     2401 ttcgaggttc gcgacgacgt gccaactgcc cggatcaaaa acggatctgg cggcatcgta
     2461 atcgaattcg gcagggaatt tcttgcgcgc gaactgggtg acgacaggga cttcctgttc
     2521 gtcctgatgc acgaaattta ccatcacgtg cttgggcacc tgcgcgtcag ccgcggcgac
     2581 cgtgtgtcac gcatgtaccg cggcctgtcg aacatcgcgg ccgacatgct ggtcaaccgg
     2641 accgtctgcc agcgcttctt tcccgatgga gtgccgctgc ttgatcggat gtatccggtt
     2701 gattcgctgc ccggcgcgct gttgcgcaat cccgggttca gctttgaaac cccggcgcac
     2761 caggatgcca ggcggcggct gatccagaat tatgaaatcg gtcttgcgaa aatgggtgcg
     2821 aatcccggtc tctgcatgcg ggtattttcg gtttaccgac tggcgtgggg gaggggcgcg
     2881 ccgtatgaaa ccgtcctgga aaggctgctg gcgctatttc gcagcatcga cccgtcgtcc
     2941 ttcccgcagg tcatcctgct gggcgaccat gaaccagggg cgggcggact gggcggcata
     3001 ttcagtatgg acggcacggg cgacaagggc gacccgttca ccggcggcgg cgaagaccgc
     3061 gaagaaatcg tccgcgtcaa aaagaccgag cacaaccagg gcgtggccgc ggccctgcgg
     3121 cgcgcgctgg aggtccaggc gaaccgctgg cgcatgcacg acccgatcgc ctgccccggc
     3181 gtggtgtgcg ttcccgggcg tcgcgacgcc gcgttcctgg gcgccggcat gtatccggtc
     3241 ttttaccagg cgaacaagac cgaattcgag ccggaacaac tggccc
//
LOCUS       DAOPPA010000377         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31754_length_3279_cov_3.644851, whole genome shotgun sequence.
ACCESSION   DAOPPA010000377 DAOPPA010000000
VERSION     DAOPPA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31754_length_3279_cov_3.644851"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PKH54_10835"
     CDS             <1..1050
                     /locus_tag="PKH54_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00432.1"
                     /translation="DMMNNIDPSLGDVALDAVMEGMEGKEELMGALMNDAMALYGGGK
                     INRSQLINLLNKYAQINEPEVSAWTPGFDISNSQAALKFQFNVDPVTFSLSYFYGRFQ
                     FPVAYWGLVDITSPGKIPEPTGKVTDTEEFVQDKFNVDVLAETYYPRMHVVGADFSYS
                     SDNKWVPGFFAEAALIIPEQVKFGFRTYLDGKLLLESSNVNVPTTPFIKATVGADWSY
                     KDWIYINVQYVRGFYDEFNDMYGIHNYFVPNVDLMFFDNELLIRLTAALNCDDVSFML
                     FPTITWTVVPAVELSVGYMGFFGPTQPKAPEELDLSNISEAVTAIIPAYASKSRFGQL
                     ASGRNVVFFKAKVSW"
     gene            1061..1993
                     /locus_tag="PKH54_10840"
     CDS             1061..1993
                     /locus_tag="PKH54_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00433.1"
                     /translation="MKTRIEFAVVLLLVGALSLAGCGWDVDGVDRMNEMYPKSDDGQS
                     QDVIEDDAEQDVEPPDATGDDTGDDVVGTDINVWPDVSYEGIEGHWAGRLVSRGKMAP
                     IGDPVDMSTSDLFLAQGTEGNLTLTFCDEIIDVNPNELFSTSTVTKQALREAIAATPI
                     ELTATAGGIDAQKIVWRWALASSIGDDDDFPEEVTLENYPDIDEDTFEGVTMSVSVVA
                     FGSPTSGERYMAKRVKFDLAAGQMSGDGRWITGAMTYVVDEKVLGASVAMLETGATIT
                     PQTEGTVYQFRRVDDQFDCAALVAGHKDLFRDAP"
     gene            2040..>3279
                     /locus_tag="PKH54_10845"
     CDS             2040..>3279
                     /locus_tag="PKH54_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00434.1"
                     /translation="MNIQPVEFKFWTDMHDFLVRRRLGALIVATMLTGVFLSRFPAIV
                     PDPNPTSFLPQDIPEVAFWQDLNKRFGALNNLMIGLEEPAGPLTSDGLTRVANITNGL
                     LALKKDGVVAARSVINVESTEVDAEGTMNVDDMVPSIPMTQTGLDELAGRIRNNIQIP
                     GAFISHDMKAYVIMVKLAEGADSGDLAGKIINVVERERGPLSSYYFGSAFATYQVSQR
                     ILDGLRWVVPVFAAGLFIVLFVGLRRPAAFLMTLLCAVIPLVWWLGTLSLLKIPVTMP
                     VLNGALLLLTFGALAFARVAEGRSRGMPVFFGSRTMLTILVGIVAFAVVAAWTDHIDM
                     SIPFLENFSLAMAVGFAVMAVAALLVVIPMMTLFDPAPELTAPLPDRKPAGIGMRAMA
                     IALPAAVAAISLSFVPSLKFA"
BASE COUNT          678 a          947 c          961 g          693 t
ORIGIN      
        1 gacatgatga acaacataga tccgtcactt ggcgacgtcg cgctggatgc agtgatggag
       61 ggaatggaag gcaaggaaga actgatgggg gccctgatga acgacgccat ggcgctttat
      121 ggcggcggaa agatcaatcg ttcccagctt atcaacctgt tgaacaagta cgcccagatc
      181 aatgaaccgg aagtcagcgc gtggacacct ggattcgata tatcgaactc ccaggccgcc
      241 cttaaattcc agttcaacgt cgatccggtt actttttcgc tgtcgtactt ctatggacgc
      301 ttccagttcc cggttgctta ctggggcctg gttgacatca ccagccccgg caagattccg
      361 gaaccgaccg gaaaggtaac ggacaccgag gaattcgtcc aggacaagtt caacgtcgat
      421 gtcctggcgg aaacgtatta tcccaggatg catgttgtcg gcgcggactt ctcgtactcg
      481 tcggacaaca agtgggtgcc cggcttcttc gcggaagcgg ccctgatcat cccggaacag
      541 gtcaagttcg gtttccggac ttatctggac ggaaaattgc tgcttgaatc gtcaaacgtc
      601 aacgtcccga cgaccccgtt catcaaggcc accgtcgggg ccgactggtc gtacaaggac
      661 tggatttaca tcaacgtcca gtacgtccgc ggattttatg acgaattcaa cgacatgtac
      721 ggcatccaca attacttcgt gccgaacgtt gatctgatgt tcttcgacaa cgagctgctg
      781 atacgcctga ccgcggcgct gaactgcgat gacgtatcgt tcatgctgtt ccccaccatc
      841 acatggacgg tcgttccggc ggtcgaactc agcgtgggct atatgggttt cttcggcccg
      901 acacagccga aggcacccga ggagcttgac ctgagcaaca tcagcgaggc cgtgaccgcg
      961 attattccgg cgtacgcgtc gaaatcgcgg ttcggccagc tcgcttccgg tcgtaacgtg
     1021 gtcttcttca aggccaaggt ttcctggtag gaggatgaag atgaagacaa ggattgaatt
     1081 cgccgttgtc ctccttttgg tcggcgcgct gtcgcttgcc gggtgcggat gggacgtgga
     1141 cggcgttgac cggatgaacg aaatgtaccc gaagtcggac gatggccagt cacaggacgt
     1201 catcgaagat gatgcggaac aagacgtcga gccccccgat gccaccggag atgacaccgg
     1261 cgatgacgtt gtgggtaccg acattaacgt atggccggac gtcagctatg aaggcattga
     1321 gggccactgg gcgggcaggc tggtttcacg tggaaaaatg gctcccatcg gggatcctgt
     1381 tgacatgtca acatcggatc tgttccttgc ccagggaacc gaaggcaacc tcacgctgac
     1441 cttctgcgac gagatcatcg atgtcaatcc caacgagttg ttcagtacat cgacagtcac
     1501 caagcaggcc ttacgggaag ccatcgcggc cacgccgatc gaactgacgg ccaccgccgg
     1561 cggaattgac gcacagaaga tcgtctggag atgggctttg gcctcatcca tcggcgacga
     1621 cgatgacttc ccggaggaag tcacccttga aaactatccc gacatcgatg aagacacctt
     1681 cgaaggcgtc acaatgtcgg tgtcggtcgt ggcgttcggc tcaccgacca gcggcgaacg
     1741 atacatggcc aagcgcgtga agttcgatct cgcggcgggc cagatgagcg gagatgggcg
     1801 ttggatcacg ggggccatga cctacgtagt tgacgaaaag gttcttggcg cgagcgtggc
     1861 gatgcttgag acgggcgcga ccatcacgcc ccagactgaa ggcaccgtgt accagttccg
     1921 gcgtgttgac gaccagttcg actgcgcggc actggtggcc ggacacaagg acttgttccg
     1981 tgacgctccg tgacagtgca gggccatatc cgtagaaccg acacacatcc ggtgatgaca
     2041 tgaacataca gcccgttgag ttcaagttct ggacagacat gcacgatttc ctggtacgac
     2101 gccgactggg agcattgatc gtcgcgacta tgctgaccgg agtcttcctt tcgcgcttcc
     2161 cggccatcgt gccggatccg aatccaacgt cgttcctgcc gcaggacatc cccgaagtcg
     2221 cgttctggca ggacctgaac aaacgcttcg gggcactgaa caacctcatg atcggtctcg
     2281 aggaaccggc cggtcccctg acatccgacg ggctgactcg agtcgcgaac atcaccaacg
     2341 ggctgcttgc cctgaagaag gatggtgtcg ttgcagcccg gagcgtgatc aacgtcgaat
     2401 cgaccgaagt cgatgccgaa ggcacgatga acgtcgatga catggttccg tcaataccga
     2461 tgacccagac cggtctggac gagcttgcag gaaggatcag gaacaacatc cagatcccgg
     2521 gggccttcat aagccacgat atgaaggcct acgtgataat ggtcaagctg gccgaaggcg
     2581 ccgactctgg tgaccttgcc ggaaaaatta tcaacgttgt ggaaagggag cgtggcccgc
     2641 tttcttcgta ctacttcggt tcggcctttg ccacatacca ggtctcgcag cgaatccttg
     2701 acggactcag atgggttgtc cctgtgttcg cggcaggcct gttcattgtc ctcttcgtcg
     2761 ggttgcggcg tcccgccgcc ttcctgatga ccctcctgtg cgccgttatc ccgctggtct
     2821 ggtggctcgg gaccctcagc ctgctgaaga tcccggtcac gatgccggtg cttaacggag
     2881 cgctcctcct tctgaccttc ggcgccctgg cattcgccag ggtggccgaa gggcgctcga
     2941 ggggaatgcc cgttttcttc ggttcccgga ccatgctgac gatcctggtc ggcatcgtcg
     3001 cgttcgctgt cgtggctgcg tggacagacc acatcgacat gtccatccct ttccttgaaa
     3061 acttcagcct ggccatggcc gtcggctttg cagtcatggc cgtggcggcc ctgctggtcg
     3121 tgatcccgat gatgacgctg tttgacccgg caccggaact tacggccccg cttccagaca
     3181 ggaaacctgc cggtattggg atgcgtgcca tggccattgc ccttccggcg gccgttgcgg
     3241 ccatttccct gtctttcgtt ccatcgctga aattcgccg
//
LOCUS       DAOPPA010000378         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31814_length_3274_cov_4.231749, whole genome shotgun sequence.
ACCESSION   DAOPPA010000378 DAOPPA010000000
VERSION     DAOPPA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31814_length_3274_cov_4.231749"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PKH54_10850"
     CDS             <1..1223
                     /locus_tag="PKH54_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOD00435.1"
                     /translation="AAIVSVWVHGLHPSVAGVDARYYFKAALDMQAGRNPYLSGDFLY
                     PPATAWLLVPAVRGLSDAALATGFNLLIYASVIMLAAGSFLLAGMKPGFGRNQDWWLI
                     PFLLAFLLFSPVTAIATGLGNISPLVGALCVWAGYLHSKNRPAGAVLLVAAATILKIV
                     PGLLLVFLFWRSIGTRDRRLLGWSLVGIFVAAAGILLPPGTADFLRGAGGDTKWGIDI
                     GGAAVSIHVWLARVLDLPALNHPDIAMVIFGIGAILASGYGYFLRHRETVAWVSVMVL
                     CDVTSPKNCPHLFLALGFPVIAMFARHTAAAFAVETTAGSEPPGRRRTSSGGFVSLLL
                     SGEAPPTTGITRLGGFFFSLALPWIAVSSEYFYMRGSLAGEIILPVIPVILLLLQTWV
                     INRWNRTEPTNVRS"
     gene            1344..2633
                     /locus_tag="PKH54_10855"
     CDS             1344..2633
                     /locus_tag="PKH54_10855"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOD00436.1"
                     /translation="MKLTIIGGGSTYTPELFEGLVSRGAELGLTEVVLHDIDPARLDP
                     VAGFCRRMAHAADACFQVHRTTELDAAISGASFVVTQIRVGGQQARLRDETIPMEFGL
                     IGQETTGAGGFAKALRTIPEILKIAGRIRELAPDAWVINFTNPSGIITETLLRHGGVK
                     AVGLCNIPMELRIESARAIGVDPSRVELDYIGLNHFGWVRAIRMDGTDITGMLLEALG
                     SGIVPANIPELDYGPRFFKALGMIPSPYLQYFYQTRRMLDTLAAETSRALVVMDLERQ
                     LFDIYRDERQSTKPELLSKRGGAWYSRVAIDVIRSLQSDMPARHVVNLANRIDVDSKP
                     IIPELPVDAVIEAPAMISSQGVRGIPAAPLPEEIIGQIRHLKSYERLTIEAATLQSER
                     KALMALVNNPLVADVGLAGRILDRLIENGEIAPTDRV"
     gene            2706..>3274
                     /locus_tag="PKH54_10860"
     CDS             2706..>3274
                     /locus_tag="PKH54_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD00437.1"
                     /translation="MSKLALFGGTPVRTEPFSPWPQFDEREEKYLLQTLRSRKWGGYP
                     YPSKLSVEFCRRFADYHGANHCVLASNGSVTLEVALKAAGIKAGDEVIVPNYTFAATA
                     ACVVRCNAVPVFVDIEERNYCIDPDLVEQAITPKTKAVIPVHLGSSCADMDRILDIAK
                     RHNLIVIEDCAHAHGGQWRGMGVGSMGDFG"
BASE COUNT          608 a          999 c          968 g          699 t
ORIGIN      
        1 ttgccgccat cgtctcggta tgggttcacg gccttcatcc gtccgtggcc ggcgttgatg
       61 cgcgctacta tttcaaggcc gctctcgata tgcaggccgg tcgcaatccc tacctgtcag
      121 gcgacttcct ttatccaccg gccaccgcat ggctgctggt gccggccgtc cgtggactgt
      181 cggacgccgc tctggcaacc gggttcaacc tgctcatcta tgcctcggta ataatgctgg
      241 ccgccggatc cttcctgctg gccggcatga agccggggtt cgggcgaaac caggactggt
      301 ggctgatacc tttcctgctg gctttcctgc tcttttcacc ggttaccgcg attgcgaccg
      361 gacttggcaa catctccccg ctggttggcg ccctttgcgt ctgggcggga taccttcatt
      421 cgaagaaccg gccggccggg gctgtactcc tggtcgccgc ggcgacaatc ctgaagatcg
      481 tgcccggtct tcttctggtc ttcctgttct ggcgttccat cggcacgcgc gacaggaggc
      541 tgcttggatg gtccctggtc ggcatttttg tcgctgctgc tggaattctc cttccacccg
      601 gaactgccga cttccttcgg ggagcgggcg gcgacacaaa atggggaatc gacatcggag
      661 gcgcggccgt cagcattcac gtctggcttg cccgcgtact ggatcttccg gcactgaacc
      721 atccggacat tgcaatggtc attttcggga tcggggctat cctggcatcg gggtacgggt
      781 atttccttcg ccacagggag accgtcgcgt gggtttcagt gatggttctt tgcgacgtga
      841 ccagtccgaa gaactgcccg caccttttcc ttgcgctggg attcccggtc atcgcgatgt
      901 ttgccaggca caccgcggcc gcctttgccg ttgagacgac tgccggaagc gagccgcccg
      961 gaaggaggcg gacatcatcg ggaggctttg tctccctgct tctttcgggc gaggcgccgc
     1021 ccacaaccgg catcaccagg ctgggcgggt tcttcttctc gctggccctg ccatggatcg
     1081 cggtctcttc ggagtacttc tacatgcgtg gaagcctggc cggggaaatc atccttccgg
     1141 tcatcccggt catactgctg cttctgcaga cgtgggttat caaccgctgg aacagaaccg
     1201 aaccaacaaa tgtcagaagc tgacattttt ccgcaatccc ctttcagtta ctattgctaa
     1261 tgcacggcag ctttggcaca atgaccctgc cccggtcggc tgttcgcatc gaaatgcccc
     1321 ggaatggcgc caggactgaa accatgaagc tcacaatcat cggcgggggc tcgacctaca
     1381 cccccgaact ttttgaaggc ctcgtctcaa ggggcgctga gttgggcctg acggaagtcg
     1441 tgcttcacga catcgatccg gcaaggctgg atcctgtcgc cggattctgc cgacgcatgg
     1501 cgcatgccgc tgatgcctgt ttccaggtgc accgaaccac agagttggat gcggcgattt
     1561 cgggcgcgtc attcgtggtg acacagatac gcgtcggggg acagcaggcc aggctgcggg
     1621 acgaaacaat cccgatggaa ttcggcctga tcggccagga gaccaccggc gctggcggct
     1681 ttgcgaaggc gctgcgcaca attccagaaa tcctgaagat tgccggccgg attcgtgagc
     1741 ttgcgccgga cgcgtgggtg atcaatttca ccaatccttc gggcatcatc accgagacac
     1801 ttttacgaca tggtggcgtg aaggcggtcg gcctgtgcaa cattccgatg gaactcagga
     1861 ttgaatcggc ccgcgccatc ggcgtcgatc cgtcccgtgt cgaactggat tacatcggac
     1921 tgaatcactt cggatgggtc agggcaatcc gaatggacgg cactgacatc accggcatgc
     1981 ttcttgaggc gctcggcagc ggcatcgtcc ccgcaaacat cccggagctt gactacgggc
     2041 cccgcttctt caaggcgctg gggatgatcc cgagtccata ccttcagtat ttctaccaga
     2101 cccgacggat gctggacacg ctggccgccg aaaccagcag ggcacttgtc gtcatggatc
     2161 tggaaaggca actgttcgat atctacaggg acgaacgtca gtccaccaag cccgaactgc
     2221 tttcgaaacg cggcggcgcc tggtactccc gcgtggcgat tgacgtcatt cgttcactgc
     2281 aatccgacat gccggcccgc cacgtcgtca acctggccaa ccggattgat gtggattcga
     2341 aaccgataat tccggaactg cccgtggacg ccgtcattga ggcgcccgcc atgataagca
     2401 gtcagggcgt ccgcggaatc ccggcagcac cgctcccgga agaaataatc ggccagatcc
     2461 ggcaccttaa gtcgtacgaa cgccttacga tcgaggcggc aacacttcag tcggaaagaa
     2521 aggcgctgat ggcgcttgtg aataatcccc tggtggccga cgtcggcctt gccggaagga
     2581 ttcttgatcg gctgattgaa aacggggaaa tcgcaccgac cgaccgggtc tgacgcgaca
     2641 tcccgataaa gtggtttttg tcggacggga attggtcctg tccggttctt caaggagata
     2701 gtgtcatgtc caagctcgct ctgtttggcg gtactcccgt caggaccgaa cctttctccc
     2761 cgtggccgca gttcgacgag cgcgaagaga agtacctgct tcagaccctg cgcagtcgga
     2821 aatggggtgg ctatccttac ccgagcaaac tttcggtgga attctgccga aggttcgccg
     2881 attaccacgg cgcgaatcac tgcgtcctgg cctctaacgg ctccgtgacc ctggaagtcg
     2941 ctctgaaggc cgccggaatc aaggccgggg acgaggtcat cgtgccgaat tacacgttcg
     3001 cggcaaccgc cgcctgcgtg gtcaggtgca acgccgttcc ggtcttcgtt gatatcgagg
     3061 aacgaaacta ctgcatcgat cctgaccttg tcgaacaggc catcacgccg aagaccaagg
     3121 ccgttattcc tgtccacctg ggaagctcct gcgccgacat ggatcgcatc ctggacatcg
     3181 cgaagcggca caacctcatc gtaatcgagg actgcgccca cgcccatggc ggtcagtggc
     3241 gtggcatggg cgtcggttcg atgggtgact tcgg
//
LOCUS       DAOPPA010000379         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31842_length_3272_cov_3.324526, whole genome shotgun sequence.
ACCESSION   DAOPPA010000379 DAOPPA010000000
VERSION     DAOPPA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31842_length_3272_cov_3.324526"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..2581
                     /locus_tag="PKH54_10865"
     CDS             419..2581
                     /locus_tag="PKH54_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00438.1"
                     /translation="MKLSRIAIAAALTTVVAAAGCGSGSNPTDDVLDDTIADIPVDSI
                     TPDVGDDMGPYDISLDTHQDSAIDTPPDLDAELPTDIGTPDIPPEVFTCGTYEDWPDQ
                     DDGQTKFGLTMFHWNIQYVPGGLVLEYNGETISICADMGLTHEDCMGLDDEALTDWII
                     RISFLPILDLYLAHPTWRATFEMPGLMVQAIAEKHPDVFAKLQCAANSGQIELVSIHW
                     SDQLFLAFPSRDLKWSIDFNRKVFEEAGLPLSGVVFNQEGQSGVGKHRIMAENGYTID
                     VMHRNLYKYHQQGDTDDGVWPVYESTGPGAPGPVKVVVGGSVDPTAGVDVDWTFFDDG
                     EVLATPGNPYLAMVTPEPDMKAVGKFEKELQEKADAGYYHTTITDYVTRLEKLGIAPK
                     PLLPFADSTWQPPSTEGISRWMGRMGTIGYSVKENDNEMRIGNYKASTCLAAADVALE
                     LAAADAIDVTADREVLDDGYLSIIKAEVSDATGINPWFGEWAYGAIHNADAMEKCQQV
                     IDSVLDKMGKQWLTVDIDTRAALQDGEYGPLFQFSPTAEAEIPISLDIQKTARPVNVD
                     WRDDGRVYQLTIDIGASDDQTGDVREQRTVIMTIPMTIDSLIYTPGLIEDQVVEIPLS
                     DFDLFADQIYVPLANGLIGVGPDLWIVKSCRSVHLAARIAWDGTDATLQFIDETVPPA
                     AQLPRVFFVVKGTAQEALGFARLINTHPNYILQHSSVR"
     gene            2597..>3272
                     /locus_tag="PKH54_10870"
     CDS             2597..>3272
                     /locus_tag="PKH54_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00439.1"
                     /translation="MKFPFRIPENLPRRILQARMTTAAFELTALALILVMEGLMAWES
                     RAFLAAFYSIVGLLIASSLYRHSLRLLQAIYKWRCLKTAVPDLILIALTLSLIPKPPL
                     MALVAGCAIIYDLVKIFRRTGAGQWFIGRFIGTPEKAVAFSFIVLILVGTILLMMPRS
                     SANGLSISFIDALFTATSAHCVTGLAVLHTGIDHLARALFAAVEPDRRLSLARQFPPL
                     RPCAFNT"
BASE COUNT          643 a         1045 c          925 g          659 t
ORIGIN      
        1 cgctgggctt ccgctggatg ggcggtgtcg cccgtgccga cctgcatgcc gatggagtcg
       61 atgacaaact ggcccgcctg gttcgcgaga agctggccat gatccgtgac gaccgcgtcc
      121 acagcaccgt tgaaggcttc cttatcgccg atgccctggc gcgcgtcctt gcctgacgaa
      181 ccatcaacgc ggattgaatg tgcctttggt tcgacgcggc tgactgcacc gtgctcgacc
      241 gctgctggct gatataatcc gcccatgcag gtacacggcg ccatccgttc atcgtgctgg
      301 ggctgcagcc cgcccgacac aagtggcgga tgcgccaggg gaacgctgcg tggtggctta
      361 ccgggcttgc aaagatcggg tataatcgcg cgtcttttcg actcacccgg gggttgccat
      421 gaaactttca agaatcgcca ttgccgccgc actgacgact gttgtcgccg ccgccggttg
      481 cggatccggt tcgaacccga ccgacgacgt gcttgacgac accatcgcgg atatcccggt
      541 tgattccatc acgccggatg ttggcgacga tatggggcct tatgacattt ccctggacac
      601 acatcaggac agcgccatcg acaccccacc cgatttggac gccgaactgc cgaccgacat
      661 cggcacgccc gatattccgc ccgaggtctt tacctgcggc acttacgaag actggcccga
      721 ccaggacgac gggcagacga agttcgggct gaccatgttc cactggaaca tccaatacgt
      781 gcccggtggc cttgtgcttg aatacaacgg cgaaacgatt tccatctgcg ccgacatggg
      841 cctgacgcat gaagactgca tgggacttga cgacgaggcc ctgaccgact ggatcatccg
      901 gatttcgttc ctgccgattc tcgaccttta cctggcacac ccgacctggc gcgccacgtt
      961 cgaaatgccc gggctgatgg ttcaggcaat cgccgaaaaa catcccgacg tctttgcgaa
     1021 gcttcagtgc gccgcaaaca gcggacagat cgaactggtc agcatccact ggtccgacca
     1081 gcttttcctg gccttcccgt cacgcgacct gaagtggtcc atcgacttca accgcaaggt
     1141 tttcgaagaa gcgggcctgc ccctttcggg cgtcgtgttc aaccaggagg gacagtccgg
     1201 cgtgggcaag caccgcatca tggcggaaaa cggttacacc atcgacgtga tgcaccgcaa
     1261 cctttacaag taccaccagc agggcgatac cgacgatgga gtgtggccgg tgtatgaaag
     1321 taccggccct ggcgctcccg gcccggtcaa ggttgtggtc ggcggcagcg ttgatcccac
     1381 ggccggtgtt gatgtggatt ggacgttctt cgacgacggc gaggttctgg caacccccgg
     1441 caacccgtat ctggcaatgg tcacgcccga gccggacatg aaggcagtcg gcaaattcga
     1501 aaaggagttg caggaaaagg cggacgccgg ctactaccat acgacgataa ccgactacgt
     1561 cacgcgcctg gagaagcttg gaatcgcccc gaagccgctg cttccgttcg ccgacagcac
     1621 gtggcagccc cccagcaccg aaggcatcag ccgctggatg ggccgcatgg gcaccatcgg
     1681 ttactcggtc aaggaaaacg acaacgagat gcgcatcggc aactacaagg catcaacttg
     1741 cctggccgcc gccgatgtcg cgcttgaact cgcggccgct gacgccatcg acgtcaccgc
     1801 cgaccgggaa gtgctggatg acggttacct gtcaatcatc aaggccgaag tctccgacgc
     1861 cacgggcatc aacccctggt tcggcgaatg ggcctatggg gccatccaca acgcggatgc
     1921 catggaaaag tgccagcagg tcatcgactc cgttctggac aagatgggca agcaatggct
     1981 gaccgtcgat atcgacaccc gcgccgccct gcaggatggt gaatacggcc ctctcttcca
     2041 gttctcgccc actgccgaag ccgaaatccc gatatccctt gatatccaga agaccgccag
     2101 gccggtcaat gtcgattggc gcgacgatgg ccgggtgtat cagttgacga ttgatatcgg
     2161 cgccagcgac gaccagaccg gcgacgtccg cgagcagcgt accgtcatca tgaccatccc
     2221 gatgacgatc gacagcctga tttacacacc gggccttatc gaggatcagg tcgtcgaaat
     2281 cccactgtcc gacttcgacc tgtttgccga ccagatttac gtcccgctgg caaacggact
     2341 gattggcgtc ggcccggatc tgtggattgt gaaaagctgc cggtcggttc acctggctgc
     2401 ccgtattgcc tgggacggaa cagatgcaac gttgcaattc atcgacgaga ccgttccgcc
     2461 cgcagcccag ttgccaaggg tcttcttcgt ggtaaagggc accgcccagg aggccctggg
     2521 atttgcccgc ctgatcaaca cgcatccgaa ttacatcctt cagcacagca gcgtgcgttg
     2581 acgaaggttc cgcatcgtga aattcccatt cagaattccg gagaacctgc cgcgcaggat
     2641 cctgcaggcc cgcatgacga ccgccgcctt cgagcttaca gcgcttgccc tgatactggt
     2701 tatggaaggt ttgatggcat gggaatcaag ggcatttctg gccgctttct attcaatagt
     2761 cggcctgctg attgcatcat cgctctatcg tcattccctt cgactgcttc aggccatcta
     2821 caagtggcgg tgcctgaaga ccgccgtccc ggatctgatc ctgattgcgc tcaccctttc
     2881 cctgatcccc aagccgcctt tgatggcgct tgtcgcggga tgcgccatca tctacgacct
     2941 tgtgaagatt ttccgccgaa ccggtgccgg tcaatggttc atcggacgat tcatcggcac
     3001 ccctgaaaag gcggtcgcct tttcgtttat cgtgctgata cttgtcggta ccatcctctt
     3061 gatgatgccg cgttcgtcgg ccaacggcct gtcgatttcc ttcatcgacg cgctttttac
     3121 cgcaacatct gcccattgcg tcaccgggct cgccgttctt cacactggca tcgaccatct
     3181 cgccagggcg ctcttcgcgg cagtcgaacc cgaccggcgg ctgtcgctgg cccgccagtt
     3241 cccgccgctg cgtccctgcg cgttcaacac gc
//
LOCUS       DAOPPA010000380         3268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_31896_length_3268_cov_4.858388, whole genome shotgun sequence.
ACCESSION   DAOPPA010000380 DAOPPA010000000
VERSION     DAOPPA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31896_length_3268_cov_4.858388"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PKH54_10875"
     CDS             <1..1575
                     /locus_tag="PKH54_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00440.1"
                     /translation="GEDGTFRVGLEKALKGGETLVVTAGYMPAGLMLVAVLRPEPVVE
                     PGSFSSPMWSWSMPVTGMDVGTKTITVEQGSGAIHVFRMAVLGMDRILEHLAKTTYGL
                     PGLALLWAPDAPWSVGLAFGKVAQKIQDGPYMPQSIFVGGGDGTSGPWGEAVILHEFG
                     HYITNNFTKHDSPGGSHYVGELISPPFAWAEAFSNFFGVSTQSQLAGAPNGRMWLIVD
                     YGKGAKAGWWIDYASGTSTVGGFKQPVAAEGMTQYLDENYLTSMLWDLWDGLEFADDD
                     ADGTALGTGRFLKAMVTDRFLDMNRGAVGVDFVDFVDSVLCAEPGLKTSVTATIKDFL
                     KFPYDGQPSCPASAAVAAVPVTMPAQAGGVSAKAAWPRNPSMTWAMGRDARFGGDASL
                     DGSLRPPLQVEVSGMKVGDEVVLTATVTVTPGLRSAPVLALSLPEGATLVKGAPVERL
                     DISQGGMVLARTFRVRALNGQVVVAVSAGDDKSGAMARAAWPALAEVRSSGIGLARPI
                     VPAVIDGVRHTTAIPF"
     gene            complement(1655..2521)
                     /locus_tag="PKH54_10880"
     CDS             complement(1655..2521)
                     /locus_tag="PKH54_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00441.1"
                     /translation="MLKFLKLILAVAAAAAIPAMASAGEEPPASTDSAAAVTQPAAEQ
                     ATEADSEPAADTTQADGEKPPASPGAAAGDAAAVDESEPPRAFNLSWTSQGAGVEPPG
                     KYTTDISAGFPGLTVMFSLPVVRHLEVDPFFSINYWDKVTSSSPLVATNIGARLKLNG
                     YDRNSLQIALVADLSFGVGLHPTNSFLMNLVFPQLLISGMVSEKVLVFGGMKVPVTLR
                     VHPDFKLGLAFAGHVGAEFLVSEKLHVFIAAEAGPEICWTFRKDPGTGQEKTASSIGA
                     WVATSVGIAVTF"
     gene            complement(2560..>3268)
                     /gene="truD"
                     /locus_tag="PKH54_10885"
     CDS             complement(2560..>3268)
                     /gene="truD"
                     /locus_tag="PKH54_10885"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOD00442.1"
                     /translation="NKLKPGHLRGNRFRIAIRGADTSCIDELKSAAATVSASGFPNLF
                     GSQRFGRNEESLVMGLGVLGIDPSAPGGRHGRRIGNFERRMSVSAVQSALFNLWVKAR
                     FADGLSRTVLAGDVLAKTVTGGMFTCEEPDTDQARLEAGEIQITGPMFGTKLMSAQGI
                     AGTREKAIQDAAGLDARAWGAAGKLAEGTRREIVVVPEAMSVDVVDDRIVFDFALPKG
                     SYASVLMREFVNDTRGI"
BASE COUNT          583 a         1011 c         1034 g          640 t
ORIGIN      
        1 ggtgaagacg gcaccttccg ggtgggactg gaaaaggccc tgaaaggtgg tgaaacgctc
       61 gtcgtaacgg cgggctacat gccggctggg ctgatgctgg tggccgtcct tcgtcccgag
      121 ccggtcgtcg agccgggctc gttctcttcg ccgatgtggt cctggagcat gccggtcacc
      181 gggatggacg tcggcacgaa gacgataacc gttgaacagg gttccggcgc gatccacgtg
      241 ttcaggatgg ccgtccttgg catggatcgg atccttgaac acctggcgaa gacgacgtac
      301 gggttgccag gcctggctct tctctgggct ccggacgcgc cgtggagcgt cggactggcc
      361 ttcggcaagg ttgcccagaa gatccaggac gggccgtaca tgccccagtc catcttcgtg
      421 ggcggcggcg acggcacttc cggtccatgg ggcgaggcgg tcatccttca cgagttcggt
      481 cattacatca cgaacaattt cacgaagcac gattcgcccg gcggaagcca ttacgtcggt
      541 gaactgattt caccgccgtt tgcgtgggcc gaggcgttct cgaacttctt cggcgtatcg
      601 acccagtcgc aactggccgg agccccgaac ggccggatgt ggctgattgt cgattacgga
      661 aagggcgcga aggccggatg gtggattgac tacgcgtcag ggacgagtac cgtcgggggc
      721 ttcaagcagc cggtcgcggc cgaaggcatg acacagtatc tggacgagaa ttacctgaca
      781 tccatgttgt gggatctgtg ggacgggctg gagttcgcgg acgacgacgc cgacggaacc
      841 gcccttggaa ccggaaggtt cctgaaggcg atggtaacgg atcgattcct tgacatgaac
      901 cgtggcgcgg tcggggtcga ttttgtcgat tttgtcgatt cggttctttg tgccgagccc
      961 ggcctgaaga cttcagtgac cgcgacaata aaggacttcc tgaagtttcc ttatgacggc
     1021 cagccgtcct gcccggcctc ggccgccgtc gcggcggttc ctgtcaccat gccggcccag
     1081 gccggcggcg tatcggcgaa ggcggcgtgg ccgcgcaatc cgtccatgac gtgggcgatg
     1141 ggcagggacg cccgctttgg cggcgatgca tcgcttgacg gttcgttaag gcctccgctg
     1201 caagtcgaag tgtcgggaat gaaggtcggc gacgaagtcg tccttacggc gacggtaacc
     1261 gtgacacccg gccttcgttc ggccccggtt cttgcgttat ccctgcccga aggggcgacc
     1321 ctggtcaagg gcgcgcccgt cgaacgcctg gacatttccc aggggggaat ggtgcttgca
     1381 cgtaccttcc gcgtcagggc cctgaacggt caggtcgttg tcgccgtgtc cgcgggtgac
     1441 gataagtccg gcgcgatggc gcgggccgcc tggccggccc tcgctgaagt gcggtcttct
     1501 ggaatcggcc tggcacgtcc cattgtcccg gccgtcatcg atggagtccg ccacaccacg
     1561 gccataccat tctgaaaagg cagggtcggt catccaggct gaatccgttt cggcgatatt
     1621 gtgtgcagct cgattcgacc tggcgatccc gacgtcagaa agtcacggcg atgccgacgc
     1681 tggttgccac ccaggcccca atcgatgaag ccgttttttc ctggccggta ccggggtcct
     1741 tacggaatgt ccagcagatt tcggggcccg cctcggcggc gatgaagacg tgcagctttt
     1801 cggaaaccag gaactcggcg cccacgtgtc cggcgaacgc aaggcccagc ttgaagtcgg
     1861 gatgaacgcg cagcgtcacg ggaaccttca ttccaccgaa taccaggacc ttttcggaaa
     1921 ccatcccgct tataagaagc tggggaaaga caaggttcat caggaagctg tttgtcggat
     1981 gcagtccgac cccgaacgac agatcggcca cgagggcgat ttgaagagag ttcctgtcat
     2041 atccgttcag cttcaggcgg gcgccgatgt ttgtcgcgac aaggggcgaa ctggaagtca
     2101 ccttgtccca gtagttgatc gagaagaacg gatcgacttc caggtgcctg acgacgggca
     2161 ggctgaacat aacggtcagc cccgggaacc cggcggaaat atcggtcgtg tatttgcctg
     2221 gcggttcgac ccctgcgccc tggctcgtcc acgaaagatt gaatgcgcgt ggcggttcgc
     2281 tttcatccac ggccgccgca tcgccggccg ccgctccggg cgaagcaggt ggtttttcgc
     2341 cgtcggcttg cgtcgtgtct gcggccggct ccgaatccgc ttcagtcgcc tgttccgccg
     2401 ccggctgggt gacggcagcc gcgctgtccg tcgatgccgg cggttcttcc ccggccgaag
     2461 ccatggccgg gatggcggca gccgccgcaa cggccaggat caatttcagg aatttcaaca
     2521 tttcgaccct cagaatccgg ccaggtgacg tgattataat catatcccgc gggtatcgtt
     2581 tacaaactcg cgcatcagga cggaagcata acttcccttt ggcagggcga aatcgaaaac
     2641 gatgcgatca tcgacgacat cgaccgacat cgcttccggc accacgacta tttcgcgccg
     2701 cgtcccctcg gcaagctttc cggcggcccc ccaggccctg gcatcaagtc cggccgcatc
     2761 ctggatcgcc ttttccctgg tgccggcgat tccctgcgcc gacatcaact ttgtcccgaa
     2821 catcggcccg gtgatctgga tttcaccggc ttccagcctg gcctgatccg tgtcgggctc
     2881 ttcacaagtg aacatgccgc cggtaacggt ttttgccagc acgtcgccgg caaggaccgt
     2941 cctggaaagg ccatccgcga accgggcctt gacccacagg ttgaaaagcg ccgactggac
     3001 ggccgaaacc gacatgcggc gttcgaagtt cccaatcctt cgtccgtgcc gcccacccgg
     3061 cgccgaaggg tcgattccaa gcacccccag tcccatcacc agggattctt cgttccggcc
     3121 gaaccgctgg ctcccgaaca ggttgggaaa gccgcttgcc gacacggtgg ccgccgccga
     3181 cttcagttcg tcgatgcacg acgtgtcggc gccacggatc gcaatccgga agcggtttcc
     3241 ccgcagatgc ccgggcttca gcttgttg
//
LOCUS       DAOPPA010000381         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32113_length_3251_cov_4.455257, whole genome shotgun sequence.
ACCESSION   DAOPPA010000381 DAOPPA010000000
VERSION     DAOPPA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32113_length_3251_cov_4.455257"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKH54_10890"
     CDS             <1..382
                     /locus_tag="PKH54_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOD00443.1"
                     /translation="AFDEFGGMAGIVTVEDIVEEIVGEIWDEHEKAEEPVKSSADGSW
                     IVKGRAAVEELAEETGIEIPDQDLYETVGGMVMTLAGKVPLKGDRVSFAGFEFEVLDR
                     TRTRVICLSVRKVPDAGQDPDEQD"
     gene            434..1750
                     /locus_tag="PKH54_10895"
     CDS             434..1750
                     /locus_tag="PKH54_10895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOD00444.1"
                     /translation="MKVGVIGTGYVGLVLGAGLAETGNHVICGDIDAEKIRILNEGGI
                     PIWEPGLDGMVLRNREKGRLRFTTDIQGLVRDSTVVFIAVGTPPQEDGSADLHHVLGC
                     AQTIATTMDGYRLIVVKSTVPVGTCDKVRDIVAANTTHPFDIASNPEFLKEGDAIQDM
                     MKPDRIVCGVDSPRARELLEDVYQTFMRTGNPILFMDVKSSEMTKYASNAMLATRISF
                     MNEMAALCDSLGADIDCVRRGMGADPRIGSKFLFAGVGYGGSCFPKDVKALASTGREC
                     GLPMKVLNAVEEVNAAQKRLLVEKVVGHFGPDLSGLKLAVWGLAFKPNTDDMREAPSI
                     EVISGLTEKGAIVSAYDPVAAVTAARVPGIGGNPRCSIVTTCAEDVLDGADALVLVTE
                     WGEFRNPDFDELKRRMKGNVIFDGRNIFDSCRVRDAGFRYYSIGRP"
     assembly_gap    1975..2074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2075..>3251)
                     /locus_tag="PKH54_10900"
     CDS             complement(<2075..>3251)
                     /locus_tag="PKH54_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00445.1"
                     /translation="DCATDPYCIEPTEETDCDDATDEDNDGVTDCDDSDCDDDPVCQE
                     PVDFCQLKAVSPFISQTGTYFQVFGWIIEAGMTDVTTGNDNPPAVKAEVGYGPKGSSP
                     DTWSWTAAFVGLGNNSPYEDADTYYYNLFLPTTAGEYDFAIRFSADSGRSWTLCDLDG
                     SDTGFDQDNQAGRMIVANDAPNLVISEYVEGTGVNNAIEITNLSGRDVMLNNCVLEGY
                     PDGTSEMAYAMAIARDGILHDRDTWVVCDDDADLALAPYCDALSGLFSVNGNDTIVLK
                     CENSLMDVFGQIGTDPGAGGWDDSGKTTVDTTMRRECYVSVGDRVRDDAFIPTAGWIH
                     FAADNYLGLGTYECSDACYQDGHEKATDACCSDGLDNDLDGISDCDETECASTDICTP
                     "
BASE COUNT          607 a          857 c         1009 g          678 t
ORIGIN      
        1 cgcgttcgac gagttcggcg ggatggccgg catcgtgacc gtcgaggaca ttgtcgagga
       61 aatcgtcggc gagatctggg atgaacacga aaaggccgag gagccggtca agtcttcggc
      121 cgatggatcc tggatcgtca agggcagggc ggccgtcgag gaacttgccg aggaaacggg
      181 catcgagatt cccgatcagg atctgtacga gacggtcggg ggcatggtga tgaccctggc
      241 cggcaaggtt ccgttgaagg gcgacagggt ttcctttgcc ggtttcgagt tcgaggtgct
      301 ggatcgaacc aggaccaggg tgatatgcct ttcggttcga aaggttccgg acgccggaca
      361 ggatccggat gagcaggact gacggcggcg tcgcgggggc gggccgcatc tttcattttc
      421 ttcctggtga atcatgaaag tcggtgttat cggtaccggg tatgttgggc ttgtgcttgg
      481 cgcggggctt gccgaaaccg ggaatcacgt catctgcggc gatattgatg ccgaaaagat
      541 ccggattctt aacgagggcg gcatcccgat atgggagccc ggccttgacg gcatggtgct
      601 gcgtaatcgc gaaaaaggcc ggttgcgctt cacgacggac atccaggggc ttgtccggga
      661 ctccaccgtc gtgttcattg cagtcggaac cccaccgcag gaagacggaa gcgccgatct
      721 tcaccatgtg ctggggtgcg cgcaaacgat tgccacgacg atggacggat atcgactgat
      781 tgtggtcaag tcaacggtgc cggtcgggac gtgcgacaag gttcgcgata ttgttgccgc
      841 aaacacgaca catccgttcg atatcgcgtc caatccggaa ttcctgaagg agggggatgc
      901 gatccaggac atgatgaagc ccgatcgcat cgtgtgcggc gtcgattcgc cgcgtgcccg
      961 tgagcttctg gaagacgtct atcagacctt catgagaacc ggaaatccaa tccttttcat
     1021 ggacgtgaaa tcgtccgaga tgaccaaata cgccagcaac gcaatgctcg cgaccaggat
     1081 ttccttcatg aacgagatgg ccgccctctg cgattcgctg ggtgccgata tcgactgcgt
     1141 tcggcgcggg atgggcgcgg atcccaggat tggttcgaaa ttcctttttg ccggcgtggg
     1201 atacggtgga tcgtgttttc ccaaggacgt aaaggccctg gcatcgaccg gccgtgaatg
     1261 cggtcttccc atgaaggtgc tcaacgcggt cgaggaagtg aacgcggcgc agaagcggct
     1321 tcttgtggaa aaagtcgtcg gtcatttcgg gccggatctt tccgggttga agctggcggt
     1381 ctgggggttg gccttcaagc cgaataccga cgacatgcgc gaggccccgt cgatcgaagt
     1441 catctcgggc cttacggaaa agggagcgat cgtgtcggcg tacgatcccg ttgcggctgt
     1501 caccgcggca cgggttcctg gaattggcgg caacccgcga tgttcgattg tcacgacctg
     1561 tgccgaagac gtgcttgacg gcgccgatgc ccttgtcctg gttactgaat ggggcgagtt
     1621 caggaacccg gatttcgacg aactgaaacg tcgaatgaag ggtaacgtca tctttgacgg
     1681 gcgaaacata ttcgattcgt gccgcgtcag ggatgccggc ttccgttact acagcatcgg
     1741 ccgcccctga catatcctga aatctgttca cccccggccg ggcattactc cagctcgacg
     1801 attcctgatt ccagtctgtg aggacggttt gaaacgactt aagtggccgg gattacgcag
     1861 ggatgcgatt tggctcgggc cggtgcggtt accggcaggc ggcgacgccc attccggcga
     1921 agtagttgtt gccgtagctg gtccactgga gggccgggtc gaacacgtcg ttgannnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgtg cagatgtcgg ttgaggcgca
     2101 ttcggtttcg tcgcagtcgg atatgccgtc gagatcgttg tccaggccgt cggaacagca
     2161 cgcatcggtg gccttttcat gtccgtcctg gtaacaggcg tccgaacatt cgtacgttcc
     2221 aagtccaaga tagttgtcgg ccgcgaagtg aatccagccc gctgtcggaa tgaaggcatc
     2281 gtcgcgcacc ctgtcgccga ccgaaacata gcattcccga cgcatggtcg tgtcaacagt
     2341 ggtcttcccg gagtcatccc agccaccggc gccgggatcg gtgccgatct ggccgaatac
     2401 gtccatcagg gagttttcgc acttaaggac gatcgtgtcg ttgccgttga ccgagaagag
     2461 gcctgacagg gcgtcgcagt atggcgccag cgcaaggtcg gcgtcgtcgt cgcaaacgac
     2521 ccatgtgtcg cggtcatgca atattccgtc acgcgcgatc gccatcgcgt aagccatttc
     2581 cgatgttccg tctgggtatc cctcgaggac gcaattgttc agcatcacgt cgcgtcccga
     2641 caggttggtg atttcaatcg cattgttgac cccggtgcct tccacgtatt cgctgatcac
     2701 caggttgggc gcgtcgttgg cgacaatcat ccgtccagcc tggttatcct ggtcgaagcc
     2761 ggtgtccgaa ccgtcaaggt cgcacagggt ccatgaacgg ccgctgtcgg cgctgaaacg
     2821 aatggcgaag tcgtattcgc cggcagtcgt cggcaggaaa aggttgtagt agtaagtatc
     2881 ggcgtcctcg tacggggaat tgtttcccag gccgacgaat gctgccgtcc aggaccatgt
     2941 gtcgggggaa ctgcccttcg ggccatatcc gacttcggcc ttgaccgctg gcgggttgtc
     3001 gttgcctgtg gtcacatccg tcatgcccgc ctcgatgatc cagccgaaaa cctggaaata
     3061 ggttccggtc tgggagatga acggtgagac ggccttcaac tggcagaaat caaccggttc
     3121 ctggcatacg ggatcgtcgt cgcagtcgga atcgtcgcag tcggttaccc cgtcgttgtc
     3181 ctcgtccgtg gcatcatcac agtcggtttc ctcggtcggt tcgatgcagt aaggatcagt
     3241 cgcgcagtcc g
//
LOCUS       DAOPPA010000382         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32173_length_3247_cov_3.857769, whole genome shotgun sequence.
ACCESSION   DAOPPA010000382 DAOPPA010000000
VERSION     DAOPPA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32173_length_3247_cov_3.857769"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKH54_10905"
     CDS             complement(<1..378)
                     /locus_tag="PKH54_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOD00446.1"
                     /translation="MKRGFVIGIAGGTGSGKTTVADMICQEVGCENVVKVVQDNYYKD
                     LSHEDFDTRARQNFDHPNAIDFSLMAKHIGMLRRGQAIDMPLYDFKTHTRKTETLRVE
                     SKDTIIVEGILVLEMDVVRREMDL"
     gene            complement(387..2693)
                     /locus_tag="PKH54_10910"
     CDS             complement(387..2693)
                     /locus_tag="PKH54_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD00447.1"
                     /translation="MSINDFAARLCQSAAAEGRNTLLEPELYSLLREGGVPVPAFYTI
                     TPAEIDRDWAATVSQVPGEKVVVKVVSPEITHKTEMGGVRVVANNRDAIADACRGIMA
                     TVAERGGPELAATIRSLIVCEKVPVGTDISSQLFAGMRFSPDMGPMLAFGFGGLEAEE
                     LAVRFKDGQGMVLASPVILDAAGMMAKFKKSFVYRKLAGLTRGGQRQIPDDELLKVFD
                     FFRMLANEFSNNPDTGWLVRDFEINPFFVSNGRLVAVDAFMRFEKTLVAERTCDIEKI
                     QRLLKPETVAILGASSKGLNPGRIILNNLQREGFPIRNIRLVRPDCEPVDGVESVRSI
                     AELPWRADLIVVAVAAKFVPDVIRETIEHEKAASMILIPGGMGETEGGKETDRAVRAL
                     IAESRAAGRYVPVFVGPNCLGIQSVPGLYDTMFIPKPKLPLPVVPDGMKQAESVLISQ
                     SGAYMITRMNIMDFVKSRYAISTGNQMDLGVADYVEAVLAEGRAEVFGLYIEGFSALD
                     GLRLAKLIRKGRAQGRDFIVYKAGRTSEGRTATSSHTASISGDYPSCAETLKDAGAMV
                     VTTLEDFNEQWKMAVTFRDRVIGGNRLSFISNAGYETVCMADSISEKPRFELAKYDDA
                     TQAAIGKILADFRLNELVSVHNPLDITPMAPDAVYTECLKAQLACSTVDACIVGIIPL
                     SVTIKSLPPGDHGPGMFTFDEPDSLVFTLPPIVAAAGKPVMATIDCGTLYQPMAQALR
                     ARGVLVFQSIDQAMRAFQKYAAYRMGID"
     gene            complement(2723..>3247)
                     /locus_tag="PKH54_10915"
     CDS             complement(2723..>3247)
                     /locus_tag="PKH54_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD00448.1"
                     /translation="YLYMLSPEGVVVSNIAPVINIPDYPDQGVVLDALYNLPRPVALD
                     AGAIATEAGVPKAENMAMAGAATAYMPFDLSDYEPIIRKLFERKGPEMVVANMKVLEN
                     GYRVGKFTRALLEAGLPGAVACRAVAMCRPDSLDVVHAPRVVELLRGQKVGEVLSKTR
                     EGLITLDDLIVELA"
BASE COUNT          708 a         1003 c          901 g          635 t
ORIGIN      
        1 cagatccatt tcacgccgca cgacatccat ttccagaaca aggatacctt cgactataat
       61 ggtgtccttc gattccacgc gcagggtctc ggtctttcgc gtatgcgtct tgaaatcata
      121 caggggcatg tcgatcgcct ggccgcgacg taacatcccg atatgtttgg ccatcaacga
      181 aaaatcaatc gcgttcgggt ggtcgaagtt ctgccgggcg cgcgtgtcga aatcctcgtg
      241 cgataagtcc ttgtagtaat tatcctggac gaccttgacg acgttttcgc agccgacttc
      301 ctgacagatc atgtccgcca cggtagtctt gccggaaccg gttcccccgg caattccaat
      361 aacgaaacca cgcttcatcg ggaacctcag tcaatgccca tccgataggc ggcgtacttc
      421 tggaacgcac gcatcgcctg atcgatactc tggaacacaa gaactcccct ggctcgcaaa
      481 gcctgggcca tcggctgata cagagtcccg cagtcgatag tggccatcac cggctttccg
      541 gccgccgcca cgattggggg caacgtaaac accagagaat ccggctcgtc aaaagtaaac
      601 atgcctgggc cgtgatcccc cggtggaagc gacttgatgg tcaccgacag cggaataatc
      661 ccgacgatgc aggcatcaac ggtggaacag gcgagttgtg ccttcaggca ttcggtatag
      721 accgcatcgg gcgccatcgg ggtgatatca agcgggttgt ggacggaaac cagttcgtta
      781 agccggaaat ccgccaggat cttcccaatc gcggcctggg ttgcatcatc gtacttcgcc
      841 agttcaaatc gcggcttttc ggaaatgctg tcggccatgc agaccgtttc atagccggcg
      901 ttagaaatga acgaaagcct gttgccgcca atcacccgat cacggaacgt gaccgccatc
      961 ttccactgct cgttgaaatc ctcgagagtg gttacgacca tcgcgccggc atccttcaac
     1021 gtttcggcac aggacggata gtcacccgaa atggaggcgg tgtgggacga cgtcgccgta
     1081 cgaccctcgc ttgtcctgcc ggccttatag acgatgaagt cacgtccctg ggcccgcccc
     1141 ttccggatca gcttcgcaag acgcagaccg tcaagcgcgc tgaagccttc gatgtaaagg
     1201 ccgaaaacct ctgcgcgacc ttcagccaga actgcctcga cataatcggc gacaccaaga
     1261 tccatctggt tcccggttga aatcgcataa cgcgacttca cgaaatccat gatgttcatg
     1321 cgggtgatca tgtacgcgcc tgactgcgaa atcaggacgg actcggcctg cttcatccca
     1381 tcgggaacaa ccggcagcgg cagtttgggc ttgggtatga acatcgtgtc gtaaagcccc
     1441 ggcaccgact ggattcccag gcagttcgga cccacaaaca caggcacgta ccggccggcc
     1501 gcgcgcgact cggcaatcag tgcgcgaacc gcgcgatcgg tttccttgcc gccttccgtt
     1561 tcgcccatgc cgccagggat caggatcatc gacgcggcct tttcatgctc tatcgtttcg
     1621 cgaatgacgt ccgggacgaa tttcgcggcc accgcgacga caatcaggtc ggcccgccag
     1681 ggaagctcgg cgattgaacg gacggactcg acaccatcaa caggctcgca atccggacgc
     1741 accagtcgga tgttccggat cgggaacccc tcgcgctgca ggttgttcag gatgatgcgc
     1801 ccggggttca agcccttcga cgaggcgcca aggatcgcga cggtttccgg cttcagcagg
     1861 cgctggatct tctcgatgtc gcaggtgcgt tccgccacca gggtcttttc aaaccgcatg
     1921 aaggcatcga cggcgacaag gcgaccgttg gacacgaaga atggattgat ttcaaaatcc
     1981 cgaaccagcc agccggtatc cgggttgttc gaaaattcgt ttgcaagcat ccggaaaaaa
     2041 tcgaaaacct tcagaagctc atcgtccgga atctgccgct gtccaccgcg ggtcaaacca
     2101 gccagttttc gataaacgaa cgacttcttg aacttggcca tcattccggc cgcgtcaagg
     2161 attaccgggg acgccagaac catgccctgc ccgtccttga accgaacggc aagctcctcc
     2221 gcctccaggc cgccaaaccc gaaggcaagc atcgggccca tgtccggaga gaaacgcatt
     2281 ccggcgaaca actggctgga gatgtcggtg ccgaccggaa ccttttcgca cacgatcagg
     2341 cttctgatgg ttgcggcaag ctcggggcct ccgcgttcgg cgactgttgc catgatgcca
     2401 cggcacgcgt ccgcgattgc gtcacgatta ttggcgacga cgcgtacccc gcccatctcg
     2461 gtcttgtggg tgatttcagg cgaaaccacc ttgacgacaa ccttctcgcc cggaacctgg
     2521 gacacagtgg cggcccagtc acgatcgatt tcggcgggcg tgatggtgta gaacgcaggc
     2581 accggaaccc cgccctcccg cagcagcgaa tacaactccg gttcaagcag cgtgtttctt
     2641 ccctcggccg ccgcggactg acacagtctt gcggcaaaat cgttgatgga catggaaaaa
     2701 cctctgtaac agcctgtcgc gactacgcaa gttcaacgat aagatcgtca agcgtaatca
     2761 gtccctcgcg agtctttgaa aggacttcgc cgaccttctg gccgcgaagc agttccacga
     2821 ccctgggcgc atgaacaacg tccagcgagt ccggccggca catggcgacg gcacggcagg
     2881 ccacggcccc gggcagaccg gcttcaagaa gcgcgcgggt gaactttccg acccgatagc
     2941 cgttttcaag aaccttcatg tttgcgacaa ccatttccgg gcccttgcgc tcgaaaagct
     3001 tgcggatgat cggttcgtag tcggaaagat cgaacggcat gtaggcggtg gctgcaccgg
     3061 ccatcgccat gttttcggcc ttgggcacgc cggcctcggt cgctatggcg cccgcgtcaa
     3121 gcgccaccgg ccgcggcaga ttgtaaagcg cgtccaggac gacaccctga tccggataat
     3181 ccgggatatt gatgaccggt gcgatattgc ttacgacaac gccttcgggc gacagcatgt
     3241 aaagata
//
LOCUS       DAOPPA010000383         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32345_length_3233_cov_3.647892, whole genome shotgun sequence.
ACCESSION   DAOPPA010000383 DAOPPA010000000
VERSION     DAOPPA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32345_length_3233_cov_3.647892"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>306
                     /locus_tag="PKH54_10920"
     CDS             <1..>306
                     /locus_tag="PKH54_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD00449.1"
                     /translation="ALFFCGVPDNEPSCLPSRPGEYNGVTSDDDDGDGVADGVDNCPL
                     VFNPIRPMDGGRQPDVDDDGVGDVCDPCPFDADTTECTGFNADDVDGDGIPNDIDNC"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <317..2163
                     /locus_tag="PKH54_10925"
     CDS             <317..2163
                     /locus_tag="PKH54_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD00450.1"
                     /translation="ENRDFDDKGDICDECPDDANFGSAACPATVWDVKSGVVQTGSKV
                     ALSNLIVTAVAMNGTKLTGFFAQEDPASPGYQGIDQSGIYVYAPSLTPIPAVGAVVSF
                     TGTVTVYFGQIQMTSISGMVIGATTMLPEPELAEPDQVMTGGARAEQLEGVLVEVQDV
                     TVTSITPPVGGGDKAPTNEFVVDNGVRVNDFIHLIQPFPAVDDQFTSIVGVLRLANDN
                     FKIEPRSADDINAVTPDLMLTGVSPLQAWIPWGDPEQATFPAFNVHLNRKVLEGETPQ
                     IEITTSIPDDLWAFASPVTIPVGQKSAEVIFSSSTPRIGLLDVDVCAKMGSQEVCNVV
                     TLVAPSFTSSITSTEPVSPAGLTVGSTMTVQVNLDVPVMAEGTTVAIDVVDPAVVSAP
                     AMAEIVVGDMFTTFTIEGLAEGETTINLAYGESTASIVIQVTDQPQTSNVMIAEVFYD
                     PSGTDSGYEWVKLYNAGAAGQDLTGWSLANGGTSWTVAKWSLSGVIPAGGCMIIGGPL
                     ANDGNGNPSYDLLLDFNPDLQNSGPSGEAADGVALFNVTADAITAASIPVDVVIYGYD
                     NLNGLVGPDGNVAPVNVGDAPSNSSIAMTDDGVWTIRATPTPGVCPPW"
     gene            2340..>3233
                     /locus_tag="PKH54_10930"
     CDS             2340..>3233
                     /locus_tag="PKH54_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00451.1"
                     /translation="MKKSLVPIQLAMLGILTAIALPALAELPLNDLYEPKASVDIIAL
                     ASYVKHVPTNVVHLGEIPGIKVEGMRGEWVSFQVLLQTNDEVTGVNLLPENLVLDDHE
                     PRVEIPAEALTLYREWFLNVVEPSGDGWAVPNHERKPGLYPDPLIPFKDPFKPGDVPV
                     AAPFGLGGTEPDLAVVWVDIQVPADAVPGNYTGYMKLVSNEVPYLGMVEIKLTVWELD
                     MPKKRNIGTSYGICEGCIRRYHGGRQESTGPEYDEIVRNYYKVLHEHRMDVTTTRGPV
                     QFQFGEDGNLLPVDWTAYDEFM"
BASE COUNT          670 a          971 c          967 g          615 t
ORIGIN      
        1 acgccctgtt cttctgcggg gtgccggaca acgaacccag ttgcctgccg agccgacccg
       61 gtgaatacaa cggggtgact tcggacgacg acgacggcga cggagtcgcg gacggggtgg
      121 acaactgccc gttggtattc aacccgataa ggccgatgga tggcggcagg cagcccgacg
      181 tggacgacga cggtgtcggc gatgtctgcg acccctgccc gttcgatgcc gacacaacgg
      241 aatgcaccgg attcaacgcc gatgacgtcg atggcgacgg cattccgaac gatatcgaca
      301 actgccnnnn nnnnnnagga aaacagggac tttgacgaca agggcgacat ctgcgacgaa
      361 tgtccggacg acgccaattt cggatccgcc gcatgtcccg ccaccgtgtg ggatgtcaag
      421 tccggcgtgg ttcagaccgg aagcaaggtc gctctgtcga acctgattgt taccgccgtc
      481 gccatgaacg gcacgaaact gaccggattc ttcgcccagg aagacccggc atcgccggga
      541 taccagggaa tcgaccagtc cgggatttac gtttatgcgc cgtccctgac cccgattccg
      601 gcggtcgggg ccgttgtttc cttcactgga accgtaaccg tttatttcgg ccagattcag
      661 atgacttcga taagcggcat ggtgattggc gcaaccacca tgcttcctga gcctgaactg
      721 gccgaaccgg atcaggtgat gaccggcggt gcgcgcgccg agcaactgga aggcgtcctt
      781 gtggaagtcc aggacgtgac ggttaccagc atcaccccgc ctgttggcgg tggcgacaag
      841 gccccgacca atgaattcgt cgttgataac ggcgtccggg tcaacgactt cattcacctg
      901 atccagccct tccccgccgt tgatgatcag ttcacgtcaa tcgttggcgt gctgcgcctt
      961 gcaaacgaca acttcaagat cgagccgcga agcgccgacg acatcaacgc ggttaccccg
     1021 gatctgatgc tgacaggcgt ttccccgctt caggcatgga ttccctgggg tgacccggaa
     1081 caggcgacat ttccggcgtt caacgttcac ctgaaccgga aggttctgga aggcgagaca
     1141 ccgcaaatcg agatcacgac cagcatcccc gatgatctgt gggcctttgc atcgccggtc
     1201 acgattcccg tcggccagaa gagcgccgag gtcattttca gcagcagcac gccgcgaatc
     1261 gggctgctgg atgtggatgt ctgtgcaaag atgggctcgc aggaggtctg caacgtcgtg
     1321 acactggttg cgccgtcgtt cacttcatcg attacttcga ccgagcctgt aagtccggcc
     1381 ggcctgacgg ttggatcaac catgacagtg caggtcaacc tggacgttcc ggttatggcc
     1441 gagggaacga cggttgcgat tgatgtcgtc gatccggccg tcgtttcggc ccccgccatg
     1501 gccgagatcg tggtgggcga catgttcacc acgttcacaa tcgagggcct tgccgaaggc
     1561 gagaccacga tcaacctggc gtatggcgaa tcgaccgcaa gcatcgttat ccaggtcacc
     1621 gaccagccgc agacaagcaa cgtcatgatc gcggaagtct tttacgaccc gtcgggaacc
     1681 gacagcggtt atgaatgggt caagctgtac aacgccggcg ctgccggcca ggatctgacc
     1741 ggatggagcc ttgccaacgg cggcacttca tggaccgtcg cgaagtggtc cctgtccggt
     1801 gtcatccccg cgggcggttg catgattatc ggcggtccgc ttgcaaacga tgggaacggc
     1861 aacccgagtt acgacctttt gctggacttc aacccggatc tgcagaattc cggcccgtcc
     1921 ggcgaggcgg ccgacggcgt ggccctgttc aacgtgacgg ccgacgcgat taccgccgcg
     1981 tcgatccccg ttgacgtggt catttatggt tacgacaacc ttaacggact tgtcggaccc
     2041 gacggcaacg tcgcgccagt caatgtcggc gatgcaccgt ccaacagctc gattgccatg
     2101 acggacgatg gtgtctggac aatcagggcg acccccaccc ccggcgtctg cccaccgtgg
     2161 tagggacagg aattccgccc gcgggaacga cattccgccc gacgtaaggg cgaatgaaaa
     2221 ttcgcccggg ccatcttgca gatccgcagc gggcggatgt agtccgcccg tacaaggcag
     2281 ggcactcgat ccgtaagggc gaatgaaaat tcgcccgttg acgcgctttc gaggtcatca
     2341 tgaaaaaatc ccttgttccc atccaacttg cgatgctcgg catcctgacg gccatcgccc
     2401 ttcccgcgct ggccgaactg ccacttaacg atctatatga gccaaaggcg tccgtcgata
     2461 tcatcgcgct ggcgtcgtac gttaagcacg ttccgaccaa tgtcgttcac ctcggggaaa
     2521 tccccggtat caaggtcgaa ggcatgcggg gtgaatgggt ttcgtttcag gttcttcttc
     2581 agacaaatga cgaagtcacg ggcgtgaacc tgttgccgga aaacctggtt ctggatgacc
     2641 acgagccacg ggtcgaaatt ccggccgagg ccctgacgct ttaccgggaa tggttcctta
     2701 atgttgttga accgtccggc gacggatggg cggtcccgaa ccacgaacgc aagccggggc
     2761 tgtacccgga cccgcttatc cccttcaagg atccgttcaa gcccggggac gtccctgtcg
     2821 cggcgccatt tggcctgggc gggacggaac cggatctcgc ggtcgtctgg gtcgatatcc
     2881 aggttccggc cgacgccgtg cccggaaatt acacgggata catgaagctc gtaagcaacg
     2941 aagttcccta tctcggaatg gtcgagatca aactgaccgt ctgggaactg gacatgccga
     3001 aaaagcgaaa catcggcaca tcgtacggca tctgtgaagg ctgcatccgc cggtatcatg
     3061 gcgggcgcca ggaatcgacc ggcccggaat acgacgaaat cgttcgcaat tactacaaag
     3121 tgctgcacga acaccgcatg gacgtcacaa cgacgcgcgg accggtgcag ttccagttcg
     3181 gcgaggacgg caacctgctt ccggtcgatt ggactgcgta cgacgagttc atg
//
LOCUS       DAOPPA010000384         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32615_length_3212_cov_4.161546, whole genome shotgun sequence.
ACCESSION   DAOPPA010000384 DAOPPA010000000
VERSION     DAOPPA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32615_length_3212_cov_4.161546"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKH54_10935"
     CDS             <1..743
                     /locus_tag="PKH54_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00452.1"
                     /translation="RSAGKSIGLKAFAWLTAPGTVVHELGHALFCVIFRHEIKEIKLF
                     DPQPDGTLGSVSHSWQKGSLYQSVGNFFIGTGPIWFGCAVVFLLSMLLVGADFLWVAV
                     GTRVEPADLATPSAAGRLSLEFLHHVWVTLSGLIEPALHSDWKFWVFIYLTLCIGLHM
                     SLSPADLKGSWVGFLMLVAVILVVNIGIAAFVTAFPASSAAAPVAGVTAAMVQFSLAV
                     TGVLLVTVIPAFILMILIAAITLAARLF"
     gene            complement(754..1302)
                     /locus_tag="PKH54_10940"
     CDS             complement(754..1302)
                     /locus_tag="PKH54_10940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOD00453.1"
                     /translation="MTRIIAISGSLRKGSFNTALLRAAAQIAPEGVLVDVATLHGIPL
                     YDGDLEDREGLPKAVRQLKDLVNGADGVIIATPEYNHSFPGVLKNALDWMSRPPTGDV
                     RVFSGKPVALTGASTGPVGTRMAQAALLPTLRALGTIPWFGRLLFVSNAADCFDESGN
                     LIDEKMKSRLAEFIRDFADFTK"
     gene            complement(1467..2696)
                     /locus_tag="PKH54_10945"
     CDS             complement(1467..2696)
                     /locus_tag="PKH54_10945"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOD00454.1"
                     /translation="MKKLINTFAGAILALVFALPLSAGAAPSFNEVDLDTDGRWTVTY
                     LGSMYDGGLNQTTFSYSVTVLDDPALSHFNVGFPLCDQLTVASWEPMGAVSIGLDPTT
                     GVYGIKWDDGQDTGTTRTYSVTFQGAIPEGTVEVAVKAGLFVAIGERPGPACHFLESG
                     DKYDISGTVFVDADLDGVLDAGEPLIPNVTVELADEDGGIVSTTITDMNGYYVFTELN
                     PGSYVVSVPVETAETDFNEQLAQFFFPTTPNLIEVELVDSDSSGNDFGFAIDTDTLIF
                     TGNGKTIGFWKHQLSVAIQGKGKAQVAAPTMAGYIAQIEALYLVHPFQFGGSFAAALS
                     VLSSTSPAAVDLLAKQLLGTEFNEVAGIGLSPEFDQLQGILIAWGEYLVANWSAFTRD
                     QLLQAKDVFDAINNTGN"
     gene            complement(2909..>3212)
                     /locus_tag="PKH54_10950"
     CDS             complement(2909..>3212)
                     /locus_tag="PKH54_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD00455.1"
                     /translation="AAMAAMVSYDWPGNIRELKAAVDYAWIHCTGDTIRPGDLPAEVL
                     ESRPVQPRRRSSDFDEKAVFMDAIARAGGNRTKAAELLGTSRATFFRRLKKLGLTS"
BASE COUNT          621 a          945 c          946 g          700 t
ORIGIN      
        1 ggcgttcggc cggcaagtcg atcggcttga aggcctttgc atggcttacg gcgcccggca
       61 cggtcgtgca cgaactgggg cacgcgcttt tctgtgtcat ctttcgtcat gaaatcaagg
      121 aaatcaagct gtttgacccg cagcccgacg gcacgctggg gtcggtttca cactcctggc
      181 agaagggcag cctttatcaa tcggttggca atttcttcat cggcaccggc ccgatctggt
      241 tcggctgcgc ggtggtgttc ctgctttcga tgcttcttgt cggcgcggac ttcctgtggg
      301 tggccgtcgg aaccagggtc gagccggcag acctggccac gccgtctgcc gccggcaggc
      361 tgagcctgga gttccttcat cacgtctggg taaccctgtc gggcttgatt gaacccgcgc
      421 ttcattccga ctggaagttc tgggtgttca tctacctgac gctttgcatc ggcctgcaca
      481 tgtccttgag cccagccgac ctgaagggat cctgggtcgg cttcctgatg cttgtcgcgg
      541 tcattcttgt ggtcaacatc ggcatcgccg ccttcgtgac ggcattcccc gcgtcttcgg
      601 ccgcggcccc ggtggccggg gtcaccgcgg caatggtcca gttctcgctg gccgtgaccg
      661 gggttcttct ggtcacggtg atcccggctt tcattctgat gatcctgatc gcggcgatta
      721 cgcttgcggc ccgtcttttc tgacaatgcg gcgctatttc gtgaagtcgg cgaagtccct
      781 gatgaattca gcgagtcttg atttcatctt ctcgtcgatc aggttgccgc tttcatcaaa
      841 gcaatctgcg gcgttcgaca cgaacagcag gcgaccaaac cagggaatcg ttcccagcgc
      901 ccgcagggtc ggcagcagtg ccgcctgggc catcctggtt ccgaccggcc ccgtgctggc
      961 tccggtcagc gcgaccggct ttcccgaaaa gacgcggacg tcgccggtcg gcgggcgcga
     1021 catccagtca agcgcgttct tcagcacgcc cggaaacgag tggttgtatt ccggcgtggc
     1081 gatgattacg ccgtccgccc cgttgaccag atccttaagc tgcctgaccg ccttcggcaa
     1141 cccttcccga tcctcaaggt cgccgtcgta aagcgggatg ccgtgaagtg tggcgacatc
     1201 cacaaggacg ccttccggcg cgatctgtgc ggccgcccgc agcagggccg tgttgaacga
     1261 accttttctc aagcttcccg aaattgcgat gatgcgagtc atttggtggc ccccaaatcc
     1321 agaattattt gttgaagcat caaacttcgt gaatcgtaac accttggaat ggaagagggt
     1381 gaaggcatta tgaaaaaggc gccgcccccg ggggttccgg gggcggcgcg cgaaacgggg
     1441 gccgaaccgg ccacccgata ctgtgatcag ttgcccgtat tgtttatggc gtcaaagacg
     1501 tccttcgcct gaagaagctg gtcgcgggtg aacgcgctcc agttcgccac aaggtattcg
     1561 ccccaggcga tcaggatgcc ctgaagctgg tcgaattccg gggacaggcc aataccggca
     1621 acttcgttga actcggttcc aagcaactgc ttcgccagaa gatccaccgc cgctggggac
     1681 gtgctggaca ggacactcaa cgcggcggcg aacgagccgc cgaactggaa cgggtgaacc
     1741 aggtaaagag cctcgatctg ggcgatgtat ccggccattg tgggcgcggc gacctgtgcc
     1801 tttcccttgc cctggatcgc gacgctcaac tggtgcttcc agaacccgat ggtcttgccg
     1861 tttccggtga agatgagcgt gtcggtgtcg attgcgaatc cgaagtcgtt gcccgaggaa
     1921 tccgaatcaa ccagttcaac ctcaatcagg ttcggggtcg tcgggaagaa gaactgcgca
     1981 agctgttcgt tgaaatcggt ctcggccgtt tccaccggaa cggaaaccac gtagcttccg
     2041 gggttcaact cggtgaaaac atagtagccg ttcatgtcgg taatcgtggt ggagacgatt
     2101 ccaccatctt catcggcaag ctcgacagtc acattcggaa tcagcggctc gccggcatca
     2161 aggacgccgt caaggtcggc atcgacgaaa accgtgcccg agatgtcata tttgtcgccc
     2221 gattcaagga aatggcaagc cggccccggt cgctcgccga ttgccacgaa cagaccggcc
     2281 ttgaccgcga cttccactgt tccttccgga atcgctccct ggaaggtcac ggagtatgtc
     2341 cgggtcgtcc ctgtatcctg gccgtcatcc cacttgattc cgtaaacgcc ggtggttgga
     2401 tccagcccga tggatacggc gcccatcggc tcccatgatg cgacggtgag ttggtcgcaa
     2461 agcgggaaac cgacattgaa atggctcaat gcgggatcat cgagaaccgt gacggagtag
     2521 gagaacgtgg tctggttcag gccgccgtca tacatggagc caaggtatgt gacggtccat
     2581 cgtccatcgg tgtccaaatc cacttcgtta aaggatggcg ccgcgccggc cgacaacgga
     2641 agcgcgaaca ccaaagccag aatcgcaccc gcaaatgtat tgattaactt tttcatgatg
     2701 atttctccca actttaacaa gcctttcaaa tcgtgccttt cgcaatgcac gaaaggttaa
     2761 aaagcaattg gcgtgccagg cgttgaatcg ctgttaaaac agtggcttgt tggcgttggg
     2821 gccggcgagt cgcccggcgg cagtctcact gtgagatttt tgccgtatca gtcattgaga
     2881 ctgtatcaaa aatgagacta aggcgggctc aggatgtcag gcccagcttt ttgagccgtc
     2941 gaaagaatgt cgcgcggctg gttccaagaa gctccgcagc cttggtccga ttgccaccgg
     3001 cccttgcaat cgcgtccatg aatacggcct tttcgtcgaa gtcggaagaa cgtcttctgg
     3061 gctgcacggg tctggactca agcacttccg cgggaagatc ccctggacgt atggtatcgc
     3121 ccgtgcagtg aatccatgcg taatcgaccg cggccttcag ttccctgatg ttgcccggcc
     3181 agtcatacga gaccattgcc gccatcgcag cc
//
LOCUS       DAOPPA010000385         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32625_length_3211_cov_4.156527, whole genome shotgun sequence.
ACCESSION   DAOPPA010000385 DAOPPA010000000
VERSION     DAOPPA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32625_length_3211_cov_4.156527"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKH54_10955"
     CDS             complement(<1..790)
                     /locus_tag="PKH54_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00456.1"
                     /translation="MFRKPAALTFMSASIVLLHAGDANAFQIGTAFTEPCHEHMSVSP
                     LSCIEGGLWGPGKTGCTGSPIFPPVDMGADHVTAAIADMFADMMGVTVESDRDRLILL
                     SLVAGCRYPDILGQSAYDLATMRPNLLSDDHEHRHFIRRSNDDGPTGDASAVQAATAF
                     IRSEIAAALAASSLPEDQRMATHTVAIDFYGEVEVTLYEPLFRLGVALHAFEDSFAHT
                     VRSGDLVTIVAVLNSIEALSGTLVESRDGIAHSMAMDRCMEQAAP"
     gene            complement(845..>3211)
                     /locus_tag="PKH54_10960"
     CDS             complement(845..>3211)
                     /locus_tag="PKH54_10960"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00457.1"
                     /translation="NISGTDVVDASGETITVADGPVAKVVTSVAEQNVVAGESTTVTC
                     KAYDQFGNELTIDMVIVVPADLTIAGNSVSGTRAGVYDIVCQPEAGTEGVELKGAKMQ
                     IVAGPATGLLLSLVPAKTNYRVGNQVKVGYTLIDDYQNPVAGAEITNPTVEPVEGVKG
                     LDDPLQFQFETEGFYTFSACVVGDPGKCDSVEAYCDGTAPLLVIESPERAAMLQGDAT
                     VMVTGTVHDDVGGLAELTINGNLVPVKDDNTFAYPMQARQGLNVIDANATDVFGLKNR
                     SMRSFLFSNVYYPAVPEDTKLSLIPNAARAYLDDKLLDNDADPADTATVAAIAEEVVS
                     KLDLIKMLPNPLLEQRVWPCDYIVFLKSITFDRPTVNAYFYEGGIGIDVVIPNFAGVG
                     RVEGDGTLCIKDDFSMSAKKLNIKVGVNIGVDAETHMLNISSRGTEVDFEDLDINIGW
                     TVDWLLGMLNSTITNVLKGVINDQVDGIINDLEADANEAIAEPIEIPIEGFIPGMADM
                     LLKVTIQPERTDWKPEGGQMDISLAVTADKRVDRDYIPGSIGRNACLSGQPEVFAFDV
                     ENPMKVNAAVFNDLVSQAAFALWYNGGLKIDLTAEAAAEMGLDLSEYGFADLVAHINA
                     LLPPILQTCGEENLVVELGDAYLELTTSNFLGRPLDMHAFLFAELEAQITARNNEDGT
                     QTLLIQILPPRRLDLDIEEINPEWYGEEQTLIDMITGLIPGLIEDPFEVDIPSFNLAE
                     LGGEDIALPNMDLFIVIEQIEQIIGHTVLGANLKLQPGAEEPTEPVTE"
BASE COUNT          644 a          964 c          936 g          667 t
ORIGIN      
        1 ccggcgcggc ctgctccatg caacgatcca tcgccatgga atgggcgatt ccgtcccggg
       61 actccacaag cgttccagaa agcgcctcga tgctgttaag aaccgcgaca atcgtgacca
      121 gatccccgga cctgacggta tgcgcaaagg aatcctcaaa cgcatgcaat gccacgccga
      181 gccggaacag aggctcgtaa agcgtgactt cgacctcgcc ataaaaatcg atggcaacgg
      241 tatgtgtggc catgcgttga tcttccggaa gcgacgacgc agccagggcg gcggcgattt
      301 cggatctgat gaaggccgtg gcggcctgga ctgccgacgc gtcacccgtc ggcccgtcat
      361 cattgctgcg cctgatgaaa tgccgatgtt catgatcatc cgaaagcagg tttggcctca
      421 tggtcgccag atcgtatgcg gactgcccga ggatgtccgg gtacctgcac ccggcaacca
      481 ggctcaacag gatcaggcga tccctgtcag attcaaccgt tacccccatc atgtctgcga
      541 acatgtcggc gatcgccgcg gtgacatggt cggcacccat atcgacaggc gggaaaatgg
      601 gcgacccggt acaaccggtc ttcccgggcc cccaaagccc gccttcgata caggacagcg
      661 gggaaaccga catatgctcg tggcacggct cggtgaaggc ggttccgatc tggaaggcgt
      721 ttgcgtcccc ggcatgaaga agaacgattg atgccgacat gaacgtcagg gctgccggtt
      781 tgcgaaacat ccctttatgc cctctgaacg gttgcggtac atggggtctt gacagggatc
      841 cggcttactc ggtgacaggt tccgtcggct cttcagcacc gggctgaagc ttcagattgg
      901 cgcccagtac ggtgtgaccg atgatctgct cgatctgctc gatgacgatg aacagatcca
      961 tgttcggaag cgcgatgtcc tcgccgccaa gttccgccag gttgaagctg ggaatatcga
     1021 cttcgaaggg atcctcgata aggccgggga taaggccggt gatcatgtcg ataagcgtct
     1081 gctcctcgcc ataccattca ggattgattt cctcgatatc caggtcaaga cggcgcggcg
     1141 gaagaatctg gatcaacagg gtctgggtcc cgtcttcgtt gttccttgcg gtgatctgcg
     1201 cttccagttc cgcgaacagg aaggcgtgca tgtccagcgg acgtcccagg aaattggacg
     1261 tggtcagttc cagatatgcg tcgcccagtt cgacgacaag gttttcttca ccgcaggtct
     1321 gaaggatggg cggcagcagc gcgttgatgt gtgcgaccag atccgcgaaa ccgtattcgg
     1381 acagatcaag gcccatttcg gccgcggctt cagccgtcag gtcaatcttc aggccgccgt
     1441 tgtaccacag cgcgaaagcc gcctggctga caaggtcgtt gaacacggcg gcattgacct
     1501 tcatcgggtt ttcaacgtcg aaggcaaaga cttcgggctg accggacagg cacgcattgc
     1561 ggccaatcga gcccgggata tagtcgcggt caacccgctt gtccgccgtg accgccaggc
     1621 tgatgtccat ctgcccgcct tccggcttcc agtctgttct ttcgggctgg atggtcacct
     1681 tcaggagcat gtcggccatg ccggggatga agccttcgat gggaatctcg attggttcgg
     1741 caatcgcctc gttcgcgtcc gcttccagat cattgatgat cccgtcaacc tggtcgttga
     1801 tgacgccctt caggacgttg gtgatcgtgc tgttcaacat ccccagcagc cagtcgaccg
     1861 tccagccgat attgatgtca agatcctcga agtcaacctc ggtaccgcgc gagctgatgt
     1921 tcagcatgtg ggtttcggca tcgacgccga tgttgacgcc gaccttgatg ttcagcttct
     1981 tggccgacat gctgaagtca tccttgatac acaatgtgcc gtcgccttcc acccgaccga
     2041 caccggcgaa attcgggatg acaacgtcga ttccgattcc gccttcatag aagtacgcgt
     2101 tgaccgtcgg ccggtcgaag gtgatcgact tcaggaaaac gatgtaatcg cagggccaga
     2161 ccctctgttc cagcagcggg ttcggcagca tcttgattag gtccagcttc gaaacgactt
     2221 cctcggcgat cgccgccacc gtggccgtgt cggcaggatc ggcgtcgttg tccaggagct
     2281 tgtcgtcaag gtacgcgcgc gcggcgttcg gaatcagcga cagcttcgtg tcttccggaa
     2341 ccgccgggta atacacgttg gagaaaagga acgaacgcat cgagcggttc ttcagtccga
     2401 agacgtcggt cgcgttcgcg tcgatgacgt tcaggccctg cctggcctgc atcgggtatg
     2461 caaaggtgtt gtcgtccttg accggcacca gattaccgtt gatcgtcagt tcggccaggc
     2521 cgccgacgtc gtcgtgaacg gtgcccgtaa ccatgacggt cgcgtcgccc tgcagcattg
     2581 ccgcgcgttc cggggattcg atcaccagca aaggcgccgt tccatcgcag tacgcctcga
     2641 ccgaatcgca ctttccggga tcaccgacga cacaggccga gaacgtatag aagccctcgg
     2701 tctcgaactg gaactgaagc ggatcgtcaa gacccttcac gccctcaacc ggctcaaccg
     2761 tcgggtttgt gatttcggcg cccgcaaccg gattctgata atcgtcaatc agggtgtatc
     2821 caaccttcac ctggttgcca accctgtaat tggtcttcgc gggaacaagc gaaagcagga
     2881 gcccggtcgc gggaccggcg acaatctgca tcttcgcgcc cttcaactcg acgccttcag
     2941 ttccggcctc cggctggcag acgatgtcat agacgccggc cctggtgccc gaaaccgaat
     3001 taccggcgat ggtaagatcg gccggaacaa caatcaccat gtcgatcgtg agttcgttgc
     3061 cgaactgatc gtacgccttg caggtgaccg ttgtcgattc accggcaacg acgttctgtt
     3121 ccgccacgct ggtcacgacc ttcgcgaccg gcccgtcggc gacggtaatc gtttcgccgg
     3181 acgcgtcaac cacgtcggtt ccggaaatgt t
//
LOCUS       DAOPPA010000386         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_32986_length_3186_cov_8.992335, whole genome shotgun sequence.
ACCESSION   DAOPPA010000386 DAOPPA010000000
VERSION     DAOPPA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32986_length_3186_cov_8.992335"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKH54_10965"
     CDS             <1..663
                     /locus_tag="PKH54_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD00458.1"
                     /translation="EDLWAFNEEVAVRAVAGSVKPVISAVGHEVDHVLTDEAADARAA
                     TPTAAGQMVVPSRDDLTATLDDCQSRATRAVVRQLDESGQILDELSGSLLSASRRILV
                     DRLEMIRVTGARLNAAHPARRLADKEHHLQTALARLDAAGRRLLERPGARLESVGLRL
                     DALNPAAPLARGYAIATDAAGNVVRSAAEVAAGDQVGVMLGKGSMDCTVVSTRPLSER
                     MD"
     gene            663..1913
                     /locus_tag="PKH54_10970"
     CDS             663..1913
                     /locus_tag="PKH54_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD00459.1"
                     /translation="MNRTRENDRGSDRNRMLELIAGIVARAPRRPPASEGELVAQQMI
                     EDEFKRLGLQTSWHGFSFHRSIFADLALHCGIAIAGTVVAMFLPWLGATLHALAAFSY
                     TMQSTRRHAVIRRLYRRRGSQNLLGVMPASDGIKPRLRLVFLGHADASYYGLVFSKQV
                     VKNTMHAPWPFNRTVALAVWMMAALVPVDLLRYVLDQHLAVVTIVQWAMTLPAAFGFL
                     LNMDVFLRNVQVPGANDNLTGSVALPVLAERLRHAKPDDVELVFAMTGCEEAGLGGAI
                     NMARQFRDKWDPENTLFVCMDGMSNGQLRYVGAEGEMFWLRPDPSLVRIVRDIAASRP
                     DYGPWRDVSPFDITIGGTDMAAFQALGYRGISFVCIDPETGGPRFYHTPDDTPANLDN
                     DALNDSVDFVEQLALKIINGGSST"
     gene            1910..>3186
                     /locus_tag="PKH54_10975"
     CDS             1910..>3186
                     /locus_tag="PKH54_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00460.1"
                     /translation="MISNRVERSLEIASKIGPDAAIGAYQYFFVRMPIRERRLCLGEI
                     RDTWRTWRADTAAGRLTDTLHFYLHVPFCSHRCSYCVYYSLASPEPAMVRAWFERLGR
                     ELPFWSEMVEAGPLSTMYIGGGTPTVLEVEDLRRLLGMVRSSFRVRPGGERSFECNPL
                     TATEDKAAIFREAGFNRVSFGIQNMHPDVLRGVNRGYQTVARTDETFRIMHDAGFHIN
                     IDLIQGLPGEPPDSIKESLRWVFERRPAEITLHRLLPTTPSGILERSIRKPLKEVSLD
                     IGDLAASSGYSVMIFDTVIKLISLSSSTHRDLLADEAAALKQSSGPDARLCFYSDVPD
                     EPSSVLAIGPDSRSQVLGGLRYRMDRYDPGLPFDGQASIAVGHEISRRDAQSLFVVGG
                     LCRKRAVDLKAFQAAFGEPLEAVFGDSMRDALDA"
BASE COUNT          573 a          921 c         1016 g          676 t
ORIGIN      
        1 gaggatctct gggccttcaa cgaggaagtg gcggtaaggg cggtcgccgg ttccgtaaag
       61 cctgtcatct cggcagttgg acacgaggtc gaccacgtct tgacggatga agccgccgac
      121 gcccgggctg caacgccaac cgccgcggga cagatggtgg tgccatcaag ggatgacttg
      181 accgcgacgc tggatgactg ccagtcccga gccaccaggg ccgtggtcag gcaacttgac
      241 gagtccgggc agatcctgga cgagttgtca ggaagtcttc tgtcggcttc acgtcgtatt
      301 ctggtcgatc ggcttgagat gattcgggtg acgggggcgc ggctgaatgc tgcccatccg
      361 gcccggcgtc ttgccgacaa ggaacatcat cttcagacgg cgctggcccg ccttgatgcg
      421 gccggccgtc gcctgctgga acgtcccggg gctcggctgg agtcggtcgg actcaggctg
      481 gacgccttga atccggcggc gccgctggca cggggatacg cgatcgcgac cgacgcggcc
      541 gggaatgtgg tccgcagcgc cgcggaagtg gcggccggtg atcaggtagg ggtcatgctg
      601 ggcaaaggtt cgatggactg tacggtcgtt tcgacaaggc ctttgtcaga acggatggat
      661 tgatgaaccg gacacgcgaa aacgacaggg gttccgatcg gaacaggatg cttgaactga
      721 tcgccgggat tgtcgcacgg gctcctcggc ggccgccggc gagtgaggga gaactggtcg
      781 ctcagcagat gatcgaagac gagttcaaac gtctcggtct gcagacgtca tggcacggct
      841 tcagttttca ccgcagcatc ttcgctgacc tggctcttca ctgcggcatt gcgatcgcgg
      901 gaacggtcgt ggcaatgttc ctgccctggc tgggcgcgac ccttcacgcc cttgccgcgt
      961 tttcttacac gatgcagtcc acccgtcggc acgccgttat ccggcgttta taccggcgtc
     1021 gcggatctca gaacctgctc ggtgtgatgc cggcatcgga tggtatcaaa cctcgacttc
     1081 gcctggtttt tctgggtcat gccgacgcct cgtactatgg gctggtgttt tcaaaacagg
     1141 tggtgaagaa cacgatgcac gccccctggc cgttcaaccg gacggtggct cttgccgtct
     1201 ggatgatggc ggcactggtg ccggtcgatc tgctgcggta tgtccttgac cagcacttgg
     1261 cggtagtgac gattgttcag tgggccatga ccctgcccgc cgccttcggt ttcttactga
     1321 acatggacgt tttcctgcgc aatgtgcagg tgcctggcgc gaacgacaac ctgaccggct
     1381 ccgtggcgct tccggttctg gccgagcgtc tgcggcacgc caagcccgac gacgtcgaac
     1441 tggttttcgc catgaccggt tgtgaggagg cggggctcgg cggcgcgatt aacatggcca
     1501 ggcagttccg tgacaagtgg gatcccgaaa acacgctgtt tgtgtgcatg gacgggatgt
     1561 ccaatggaca gctcaggtac gtcggcgccg agggcgagat gttctggctg cgaccggatc
     1621 cctcgctcgt gcggatcgtg cgcgatattg ccgcatccag gccggactac gggccgtgga
     1681 gggatgtttc tccatttgac atcaccatag gcggaaccga catggccgcg tttcaggcgc
     1741 ttggatacag gggaatttcg ttcgtttgca tcgaccccga gaccggtggg cccaggttct
     1801 accatacgcc ggacgacacc cccgcgaatc tggacaatga cgccctgaac gattctgtag
     1861 attttgtcga gcaactcgct ctgaaaatca tcaatggcgg ctcttcaaca tgatctcgaa
     1921 tcgcgtggaa agatcgctgg aaattgcgtc gaagatcgga cctgacgccg cgatcggcgc
     1981 ttatcagtac tttttcgtcc ggatgccgat ccgtgagcgg cgcctctgcc ttggcgagat
     2041 aagggatacc tggcgaacct ggcgtgcgga caccgcggcc ggacgtctga ccgacacgct
     2101 gcatttctac ctgcatgtgc cgttctgcag tcatcgttgt tcatattgcg tctattacag
     2161 ccttgcttcg cccgaaccgg ctatggtgag ggcatggttt gagcgtctgg ggcgggaact
     2221 gcctttctgg tcagaaatgg tcgaagctgg gcctctctcg accatgtata tcggcggtgg
     2281 aacccccacg gtgctcgagg tcgaagacct gcgtcgtctg ctcgggatgg tgagatccag
     2341 ctttcgggtc aggccgggtg gagaacgcag cttcgagtgc aatcccctta cggcaacgga
     2401 agacaaggcc gccatctttc gtgaggccgg cttcaaccgt gtcagtttcg gcatccagaa
     2461 catgcatccg gacgttttgc gcggcgtcaa tcgcgggtac cagacggtcg cacgcactga
     2521 cgaaaccttc aggataatgc acgacgccgg ttttcacatc aacatcgacc tgattcaggg
     2581 gctcccgggg gaaccaccag actcgataaa agagagcctg cgctgggtat ttgagcggcg
     2641 tcccgccgag attacgcttc atcggctcct gccgaccacg ccctcgggga tactggagag
     2701 gtcgatacgg aaacccctca aagaggtttc tctcgatatc ggcgacctgg ccgcttcgtc
     2761 cggttactcg gtgatgatct tcgacaccgt gatcaagttg atctcgcttt ccagttcaac
     2821 gcaccgagac cttctggcag acgaagccgc cgcgttgaag cagtcttccg gacctgacgc
     2881 aaggttgtgc ttctattccg acgtcccgga cgagccgtct tcagtgcttg ccatcggtcc
     2941 cgactccaga tcgcaggtgc ttggcgggct tcgataccgc atggacaggt acgatccagg
     3001 tctgccgttt gacgggcagg cctccatcgc tgtcggacat gagatttcaa ggcgggacgc
     3061 tcagtcattg ttcgtggtcg ggggtctgtg tcgcaagcgc gcggtggacc tcaaagcctt
     3121 tcaagccgcc ttcggggagc cactggaagc cgtgttcggg gattcgatgc gggatgcgct
     3181 cgacgc
//
LOCUS       DAOPPA010000387         3157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33409_length_3157_cov_4.326241, whole genome shotgun sequence.
ACCESSION   DAOPPA010000387 DAOPPA010000000
VERSION     DAOPPA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33409_length_3157_cov_4.326241"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKH54_10980"
     CDS             complement(<1..612)
                     /locus_tag="PKH54_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00461.1"
                     /translation="MKTFTRPFAVACGIVMTLAVSCGGSDPISDIETPDISLPDTINP
                     DVPRDTGTTDDSVRDQAEESSEPDTGTDSIADRFEPDVEPECPGSYGCPCNSAAECLS
                     GMCVDTMDESVCSRHCSGSSPCPDGWTCTNVNQAGGDPVYGCVYSFPTLCRPCKADSE
                     CIPALGAGNRVFKCLTAGDTGSFCGAECLEGRKCPEGFRCEDQG"
     gene            775..1281
                     /locus_tag="PKH54_10985"
     CDS             775..1281
                     /locus_tag="PKH54_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00462.1"
                     /translation="MKLHKVLLVVLVAIAASSAWGCDDEEGCPACAGEGCVTLDQALD
                     AWLTLIQDCSCFNPQEDREGWAARWAAVPPALVNACATSIMCDKTPHTCGESEDPLSW
                     GDPDGLEACMRRFVDFGQTACYGDRLNITHDMLGCISVDCNDYCGTEGGGCVTGESAS
                     DCGCGFVK"
     gene            1299..2549
                     /locus_tag="PKH54_10990"
     CDS             1299..2549
                     /locus_tag="PKH54_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00463.1"
                     /translation="MKYVLFSLMMASVLFMAGCSGGDAGSDLVAPDVTSDAAIAGDVP
                     DVADVSAGSDVSNDVTVARADYSAFGPYPVGRLHVELHDAARDRTLPVEIWYPATSAA
                     KADFDLGGPITDVWTESENALIAPYLENASPCLTTTVHAVAGAEPAPGSWPLVVFSHC
                     STCTRTSNVSAVERLASHGFAVVAADHVGDTLWDSIRDGTSSGLAEEFLVVRGADVVF
                     LLDTFLDPQDDSVPESVRGRFDPERVAMFGHSFGAVTSGLVTATDARIRGAVLHAAPF
                     AGHYYLVQPETNTRPVFTIVAVEDSMITWVGNDMLREDFSRLSGPAWKAEVADAGHLS
                     FHDLCRVRGSFADCCGTGARQSDQSFEVFEYIDQAESMQIGATMTTAFLAWLLLGDES
                     ARSVFEQSPDPRVSVESRNTALLD"
     gene            2560..>3157
                     /locus_tag="PKH54_10995"
     CDS             2560..>3157
                     /locus_tag="PKH54_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00464.1"
                     /translation="MKPSGIRQFIPDMVLCALLCLVITVHFTIGFSRLGYIVWERFES
                     HRDDAASVVTSDPCEGVRFSPADPDPALACLPPFVDPAGGLSAGDRPAGHLLFLVMFL
                     VGMPLLIGLAVWRGRREIAAFFREPMLRMPGDRPATRREGILLAAILSMAVFFRVFML
                     GSWVVEESEWGAVERLPLLDLVFHGREIRHEPPLMENQV"
BASE COUNT          533 a          936 c         1002 g          686 t
ORIGIN      
        1 tccctggtct tcgcatctga atccttccgg gcacttgcgg ccttcaaggc actcggcgcc
       61 gcagaaactg ccggtgtcgc ccgcggtaag gcatttgaag acgcggttgc cggccccaag
      121 tgccgggatg cattcagaat ccgccttgca gggcctgcaa agggtcggga agctatagac
      181 gcagccataa acagggtcgc cgcccgcctg gttcacgttt gtgcaggtcc atccgtccgg
      241 acacggcgag gaaccgctgc aatgacggga acacaccgat tcgtccatcg tatcgacgca
      301 catgccgctt aggcactcgg ccgccgaatt gcagggacat ccgtaactgc ccggacattc
      361 gggttcaacg tccggctcaa acctgtcggc gatgctgtcc gtgccggtgt caggctcgga
      421 cgattcctcc gcctggtcgc gaacgctgtc gtccgtcgtg ccggtgtccc ggggcacgtc
      481 gggattgatc gtgtcgggaa gtgaaatatc aggcgtttcg atgtcggata tcgggtcgga
      541 accgccgcac gaaaccgcaa gcgtcatcac aatgccgcat gcaaccgcaa atggccttgt
      601 aaaggtcttc atgttggccc tcgctttcag ggattaaaat ccaggcaatt ataggttaaa
      661 tctatagcct gttcaacgtg atcgtcctgc ttccgccgtg cgctttccga tgtcggttta
      721 catcgccggc atccttaagg ataatgcacg gctggcatat ttccccggtg attcatgaaa
      781 ctgcacaaag tcctgcttgt cgtgctcgtt gcaattgcgg cgtcctccgc atggggatgc
      841 gatgatgaag aagggtgtcc ggcctgtgcc ggcgaaggat gcgtgacgct tgaccaggcg
      901 ctggatgcat ggttgaccct gatccaggac tgcagttgct tcaatccgca ggaggatcgc
      961 gagggctggg cggcgcggtg ggctgccgtt ccacccgcgc ttgtcaacgc gtgtgcaaca
     1021 tccatcatgt gtgacaagac gccacacaca tgcggggaaa gcgaggatcc actttcgtgg
     1081 ggtgatcccg acggcctgga agcctgcatg cgtcggttcg tcgatttcgg gcagaccgcc
     1141 tgttacggtg acaggttgaa tatcacgcac gacatgcttg gctgtatctc ggtcgactgc
     1201 aacgattact gcggaaccga aggtggcggt tgcgtgaccg gtgagtccgc ttccgactgc
     1261 ggatgtggct tcgtgaaatg aatccacggg gggcaatggt gaaatacgtc ctgttttcat
     1321 tgatgatggc gtcagtgctt ttcatggcgg gatgttcggg cggcgatgcc gggtcggacc
     1381 tggtcgcgcc cgacgtgaca tccgatgcgg ccatcgcggg cgacgtgccc gacgtggccg
     1441 acgtttcggc gggatcggac gtttcaaatg acgtcacggt ggcgcgcgcc gattactcgg
     1501 cgttcggccc ttatccggtc ggacggctgc acgtggaact tcatgacgcc gcccgtgatc
     1561 ggacgcttcc ggtcgaaatc tggtacccgg cgacatcggc ggcaaaggcc gatttcgacc
     1621 tgggcggccc gatcaccgac gtgtggacgg aatccgaaaa cgccctgatc gccccatatc
     1681 ttgaaaacgc atcgccatgc cttaccacga cggttcacgc ggtggccggc gcggagccgg
     1741 caccgggttc gtggcccctg gtggtttttt cccattgttc gacctgcacg cgtacatcga
     1801 acgtatcggc cgtggagcgt cttgccagtc atgggtttgc cgtggtcgca gcggatcatg
     1861 tcggcgatac cctgtgggac tcgatcaggg acggaaccag ctccgggctg gcggaagaat
     1921 tcctggtcgt tcgcggcgcg gacgtggttt tcctgctgga cacgtttctt gatccgcagg
     1981 acgactcggt tcccgaatcg gtgcgcggcc gcttcgaccc ggaaagggtc gcgatgttcg
     2041 ggcacagttt cggcgcggtc acgtcaggcc tggtcacggc caccgacgcc aggatccgtg
     2101 gcgccgtgct tcatgccgcg ccgtttgccg gccattacta cctggttcag ccggaaacaa
     2161 acaccaggcc cgttttcacg attgtcgcgg tcgaggacag catgatcacc tgggtcggga
     2221 acgacatgct tcgcgaggac ttctcacggc tttccggtcc cgcctggaag gccgaggtcg
     2281 ccgatgccgg tcatctttcc ttccacgatc tgtgcagggt tcgcggcagt ttcgcggact
     2341 gttgcggcac cggcgcgcgt cagagcgacc agagtttcga ggtattcgaa tacatagatc
     2401 aggccgagtc gatgcagatt ggcgcgacca tgacgacggc cttcctggcg tggctgcttc
     2461 tgggcgacga atctgcacgc tccgtcttcg agcagtcgcc ggatcccagg gtcagcgtcg
     2521 aatccaggaa cacggcactg ctggactgag gttagccgat tgaagccttc cggtatcagg
     2581 cagttcatcc cggacatggt cttgtgcgcg ctcctttgtc ttgtgataac ggttcacttc
     2641 acgatcggct tttccaggct tggctacatc gtgtgggaac ggttcgaatc gcaccgcgac
     2701 gacgcggcgt cggtcgtgac gtccgatccc tgtgaaggcg tccggttctc gccggcagat
     2761 ccggatccgg cgctggcatg ccttccaccg ttcgtcgatc cggccggcgg actgtcggcc
     2821 ggggacaggc cggcggggca cttacttttc ctggtgatgt tccttgtcgg catgccgttg
     2881 ttgatcggtc tggcggtctg gcgcggccgg cgcgaaatcg ccgcgttctt tcgcgagccc
     2941 atgctccgga tgccgggcga tcgccccgca acccgccgtg aaggcatcct gcttgccgca
     3001 atcctgtcga tggccgtctt tttccgtgtc ttcatgcttg gatcctgggt ggtcgaggaa
     3061 tccgagtggg gcgcggttga aaggcttccc ctgcttgacc tggttttcca tgggcgcgag
     3121 attcgtcacg aaccgccgct catggaaaac caggtca
//
LOCUS       DAOPPA010000388         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33539_length_3147_cov_3.723803, whole genome shotgun sequence.
ACCESSION   DAOPPA010000388 DAOPPA010000000
VERSION     DAOPPA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33539_length_3147_cov_3.723803"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKH54_11000"
     CDS             complement(<1..792)
                     /locus_tag="PKH54_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00465.1"
                     /translation="MKEQPRKLIGIIAETAGVALVILIVTLLLAEGIIRLVNPFPKVQ
                     VIRRDRIEMFQDGDVPIWIQDLKRMNLDCPDMHPDARLVVFSGDSIFFGSGLQTEDAF
                     TTLMQQELDRRDGPGAWCILNLSQPGYSVEQKIYWLARLMKRHTPDLVYWEVWPNESN
                     RYRMIGDAAYNMAGVDGDTGRLPGFVPGPEWLRTWLFLNSRFFEYMVLTMFELEPFRS
                     SWVTGVYLPMLEEYRAALRARGSDFSLVVATRLDMPFSMMRSPRLD"
     gene            complement(789..2933)
                     /locus_tag="PKH54_11005"
     CDS             complement(789..2933)
                     /locus_tag="PKH54_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOD00466.1"
                     /translation="MPRKSGRRSSRKSAAPGIFAGLFAVGAVIFLLAAAAVVKLTEAG
                     LPPVPTWEEYSASIPKVSRIRAADGTILAEIFSERRTVLDPESLPPLLVNAVLAAEDA
                     GFMDHQGLSYVGIARAMLNNLWRGRISQGGSTITQQVVKQVSLSPERTFKRKFRELLL
                     TRRFEIALTKRQILAMYMSTIYLGHGRYGFEEAARFYFGRKASELAVHEAAMLAGLIS
                     SPEGNSILKNPDGAVARQRHVLNRMAELGMITADQATAAAGTSLLVWGRDDPMLGTAP
                     YFVDAVLRDLRALVGKDALDKGGLDVVTTLDLEVQAAVDKSIAEGLVRLYAAGRQRLA
                     DEAAANMVMNEETGERLPPPPRAIATRIESCRRGDRQAFVKVGDSRKVVANRTLARMG
                     GTVDSQLDALCDNKAPIAVSKGVGEVESVRGKLEYVNAELGPQASMVVIDSRTRAVRA
                     MTGGEDFETRRFNRAVQSRMPIGSTIKPFIFAAALAAGIPADKGWVNEPVAFRGHAGK
                     LWKPRNYGGVYDGRTYDMTGALKDSINVVAVKILKDVGVDRVGELMESIGFSGSIPRD
                     LSMALGSVEASPMELANAYAVFGSGGFHDAAWTVRRVTDFRGKVLAEHQSRSGRVLPA
                     DVATAMKVMMREVAVGGTAKALADLPGSVWGKTGTSSMSREAWFAGGFRELVAVVLVA
                     YDDRLAMSGATGGNTAVPFFRSFAAAILGEGR"
     gene            complement(3036..>3147)
                     /locus_tag="PKH54_11010"
     CDS             complement(3036..>3147)
                     /locus_tag="PKH54_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOD00467.1"
                     /translation="ITPVPGGVGPMTIAYLMRNTVIAACLRRGIDFPSGL"
BASE COUNT          646 a         1024 c          891 g          586 t
ORIGIN      
        1 atcgagcctg ggactcctca tcatcgaaaa cggcatgtcc agcctcgtcg cgaccacaag
       61 tgagaagtcc gatccccttg cgcgcagggc ggcgcggtat tcttccagca tcggaaggta
      121 aacgccggtg acccatgatg acctgaacgg ctccagctcg aacatggtca gaaccatgta
      181 ctcgaagaac cttgaattca ggaacagcca ggttcgaagc cactcgggac cgggaacgaa
      241 tcccggaagc ctgcccgtgt cgccatcgac gccagccatg ttgtacgcgg cgtccccaat
      301 catgcggtaa cggttcgact cgttcggcca gacctcccaa tagaccagat ccggcgtatg
      361 cctcttcatc agtcttgcaa gccagtagat cttctgttca acggaatacc ccggctggga
      421 caggttcaga atgcaccagg cgcccgggcc gtcccggcga tccagttcct gctgcatcag
      481 cgtcgtgaag gcgtcttcgg tctggagccc ggaaccgaaa aaaatcgaat cgccggaaaa
      541 cacgacaagc ctggcgtccg gatgcatgtc cggacagtcc agattcatcc ttttcagatc
      601 ctgtatccag atcgggacat ccccgtcctg aaacatctcg atcctgtcgc gccttatgac
      661 ctgaaccttt ggaaacggat tcaccagcct tatgatgcct tccgccagaa gcaatgtgac
      721 aatcaggatg acaagtgcca cgccggcggt ttccgcaatg attccaataa gcttgcgcgg
      781 ttgttctttc atctgccctc gccaaggatg gccgccgcga acgaccggaa aaacggaacg
      841 gcggtattgc cgcccgttgc ccccgacatg gcaagcctgt cgtcatacgc gacaagcacc
      901 acggcaacca gttccctgaa cccgccggca aaccaggcct cgcgtgacat gctgctggtg
      961 ccggtcttgc cccagacgct gccgggcagg tcggccagtg ccttcgcggt tcccccgacg
     1021 gcgacctccc tcatcatgac cttcatggcc gtggcaacat cggccggaag caccctgcca
     1081 gaccggctct gatgttcggc aagcaccttg cccctgaagt cggtgacgcg tcgaacggtc
     1141 caggcggcat catggaatcc tccggagccg aatacggcat aggcgttcgc aagttccatc
     1201 ggactggctt ccacgctccc cagggccatg gaaagatcac gcggaatgct gccggaaaag
     1261 ccaatggact ccatgagttc gccaacgcga tccaccccga catccttcag gatcttgacg
     1321 gcaacgacat tgatggaatc cttcaacgcc cctgtcatgt cgtatgtccg gccgtcatag
     1381 accccgccgt agttgcgcgg cttccagagc ttgccggcat gcccccggaa cgccacgggc
     1441 tcgttgaccc agcccttgtc cgctggaatt ccggcagcca gggcggccgc gaatatgaaa
     1501 ggcttgatgg tcgaaccgat tggcatccgg gactgcaccg ccctgttgaa ccggcgggtt
     1561 tcaaaatcct cgcccccggt catcgccctg accgcccggg tgcgcgaatc aatcaccacc
     1621 atcgatgcct ggggaccgag ttcggcgttg acgtattcaa gttttccacg gacggattcg
     1681 acctcgccga cccccttcga aacggcaatc ggggccttgt tgtcgcacaa cgcgtcaagt
     1741 tgcgaatcga ccgtgccgcc catcctcgcc agagtcctgt ttgcgacgac cttccgtgaa
     1801 tctccgacct tgacgaacgc ctgccgatcc ccacgccgac aactttcaat ccgggtagct
     1861 atcgccctgg gaggaggggg aagcctttcg cccgtttcct cgttcattac catattggcc
     1921 gcggcttcgt cggccaggcg ctgacgcccg gctgcgtaaa gacgcaccag cccttccgct
     1981 atcgacttat cgaccgccgc ctgcacttca aggtcaagcg tggtcacgac gtccagtcca
     2041 cctttgtcaa gcgcgtcctt gccaaccagg gcgcgcaggt cgcgcaacac ggcatcgacg
     2101 aagtaaggcg cggttcccag catgggatcg tcccggcccc acacgagcag ggacgttccg
     2161 gccgccgccg tagcctgatc cgccgtaatc atgcccagtt cggccatccg gttcagcacg
     2221 tgacgctggc gggccactgc gccgtcggga tttttcagga tggaatttcc ctcgggggac
     2281 gaaataaggc ctgcaagcat ggcggcctcg tgaaccgcca gttcggacgc cttcctgccg
     2341 aaatagaaac gggcggcttc ctcaaatccg tatcgcccgt gccccaggta gattgttgac
     2401 atatacatcg ccagaatctg gcgcttggtc agggcgatct cgaaccgccg ggtaagcagc
     2461 agttcccgga acttccgctt gaacgtgcgc tccggggaca gagagacctg cttgaccacc
     2521 tgctgcgtga tggtcgagcc accctgggag atgcgccccc tccagaggtt gttcagcatt
     2581 gccctggcga ttccgacata cgaaaggccc tggtgatcca tgaacccggc gtcctcggcc
     2641 gcaagcacgg cattcacaag aaggggcgga agggattcgg gatcaagaac cgtccggcgc
     2701 tcggagaaga tctcggcgag gatggtgccg tccgcggcgc gaatcctgga aaccttcgga
     2761 attgaagctg agtattcctc ccaggtcgga accggcggaa gcccggcctc ggtcagcttc
     2821 acgacagccg ccgcggcaag aaggaatata acggcgccga ctgcaaatag tcccgcgaaa
     2881 atcccgggtg ctgccgactt ccttgaactt cgcctgcccg acttgcgtgg cattgattcc
     2941 tcgggatggt tcagaaccgg gacgatttta gcatccacgg tagcaaccgg ccacaattcg
     3001 ccgttacgct tgaaaatgac cgcggggctg gacggctaga ggccggaagg gaaatcgatg
     3061 ccgcgacgca ggcatgcggc aatcacggta ttccgcatca gataggcgat tgtcatggga
     3121 ccgacaccgc cgggaaccgg ggtgatc
//
LOCUS       DAOPPA010000389         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33553_length_3146_cov_3.799418, whole genome shotgun sequence.
ACCESSION   DAOPPA010000389 DAOPPA010000000
VERSION     DAOPPA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33553_length_3146_cov_3.799418"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKH54_11015"
     CDS             <1..543
                     /locus_tag="PKH54_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00468.1"
                     /translation="DLNVNQDQGGTDTTQACPTGVPTGPSCLEVAACAVLCDDAAFET
                     TCLAQAETAAADAWAALKACAATNECIAMFQAEQVSDCLIGSCGTEAGACVVGESTCR
                     DIWTCRKDCPAEDPTCAMKCIASGTTEAQEQWVEYKNCILDIDCSSEDVMENGWPTFT
                     CEQYARGFCSLPYQACFPPT"
     gene            complement(546..1124)
                     /locus_tag="PKH54_11020"
     CDS             complement(546..1124)
                     /locus_tag="PKH54_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00469.1"
                     /translation="MSQQIPVWTANRGTMMMVKVALGQSGRTPIFVNSQAELERTMAE
                     SGSRLAIVGLAELQAAGLTVQALTGRFARETGWVVMVVGLPPPDGFGPGVKTLKVPFS
                     AAELRQLIDSDPAPAGSVRPSTPGTQDLTAIVRAEIERIVREKADAMVADAVMKMVPE
                     LAEAMIRSELDRLLSDAAEKAVSTDPAPDDET"
     gene            complement(1155..1507)
                     /gene="rnpB"
                     /locus_tag="PKH54_11025"
     ncRNA           complement(1155..1507)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH54_11025"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1557..2666)
                     /locus_tag="PKH54_11030"
     CDS             complement(1557..2666)
                     /locus_tag="PKH54_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD00470.1"
                     /translation="MAQNIVSPGRRERPGLQAGVVQAFACLGKTFSGRPIAIAKRALT
                     LQSLQRQLLAGIPVVAAFKSLGGLSGGRTGIALNRVAAGLESGLTLADAARLAPQIFP
                     GTAPDLIGAAEHTGTIPDMLDSMVRTLKLQAETRRRILTSFAYPAFLALSACLLLPLP
                     RLVSCGPGGYLAGVAKTALPFLAIAGIIGSFWFATLNPRASDALRRIARHVPLVGAAI
                     RERALFASLQTIGLALQAGLGISQSLKLGTIAAQDRQTSDAWKATALLVARGATLSAA
                     LHPSLPDDIAVSIGTGEKTGALSDAILEAAVSTWSRYQARMALLARIASVTISVLVVS
                     FIAFNIVQSFLDVLNLGGNSQMDMEMMRELRGVIQ"
     gene            2777..>3146
                     /locus_tag="PKH54_11035"
     CDS             2777..>3146
                     /locus_tag="PKH54_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00471.1"
                     /translation="MTRIALIAVGTVVLATSACSVPTRVQKLALHASAPGLAGESGPF
                     AVLDPGTRPESRDSATDGGRNPEKDKPAASRQASGGASQRAAAPTPAVRQPAPARTAV
                     LMVPASGDARARLARSARRLV"
BASE COUNT          600 a          971 c          984 g          591 t
ORIGIN      
        1 gatctgaacg tcaatcagga tcagggcgga accgatacga cccaggcttg cccgaccggc
       61 gtgccgacag ggccaagctg ccttgaagtc gcggcttgtg cggttctgtg cgatgacgcc
      121 gcgtttgaaa cgacgtgtct tgcccaggca gagacggcgg ccgccgacgc gtgggctgcc
      181 cttaaggcgt gtgcggcaac aaacgagtgc atagcgatgt tccaggccga gcaggtctcc
      241 gattgcctta tcggttcatg cggcaccgag gccggcgcct gcgtggtcgg tgaatcgacc
      301 tgccgcgata tatggacctg tcgcaaggat tgccccgccg aagacccgac ctgcgccatg
      361 aagtgcatcg cctcgggaac aaccgaggcc caggaacagt gggttgagta caagaactgc
      421 atactggaca tcgactgttc cagtgaggac gtcatggaga atgggtggcc gaccttcacg
      481 tgcgagcagt atgccagggg cttctgcagt cttccttacc aggcttgttt cccgccgaca
      541 tgatgtcagg tttcgtcgtc cggtgcggga tccgttgata ccgccttctc ggcggcatcg
      601 ctcagcaggc gatccagttc cgatctgatc attgcttccg ccagttccgg aaccatcttc
      661 atcaccgcgt cagcgaccat cgcgtcggcc ttctcgcgga ctatccgttc gatttcggcc
      721 ctgacgatcg cggtcaaatc ctgcgtcccc ggggttgacg gccggacgga gccggccggc
      781 gcgggatcgg aatcgatcaa ctggcgcagt tccgcggcgg aaaaaggcac cttcaaagtc
      841 tttacgcccg ggccgaaccc gtcgggtgga ggaagcccga caaccatcac gacccatcca
      901 gtttcccttg caaagcggcc ggtcagcgcc tggacggtca gcccggcggc ctgaagttcg
      961 gcaagcccga ctatggcaag gcggcttcca gactcggcca tggtgcgttc gagttcggcc
     1021 tgagagttga cgaaaatcgg cgtgcgtcct gactggccaa gcgcgacctt gaccatcatc
     1081 atggttccgc ggttggccgt ccagaccgga atctgctggg acatctggtt tcctccgtga
     1141 attcgccggt tgtcgatcat ggcggcccgt aagccgggtt ctgtcggaac cgaagttccc
     1201 gacggtcatt catctgagcg ttccgttacc ggacgcttca agcaacctta cccgggggca
     1261 tcgggtgggc ggcccgcccc ctgttcggtc ttgctccggg tggggtttac cgatcgccag
     1321 gttgccctgg cgacccgtgg tctcttacac cgcggtttca cccttaccct tccgccaggc
     1381 ggaaaggcgg tctggtttct gtggcacttt ccctggggtt acccccgccg ggcgttaccc
     1441 ggcaccctgc cctgtggagc ccggactttc ctccggccgc aaagccggcg accgtccagg
     1501 ccaccatgat cgacaaggaa ggataccact gtgtgaaggc ttgaaacagc cgtcagttac
     1561 tgaatcaccc cacgcagttc gcgcatcatt tccatgtcca tctgggaatt cccgccaagg
     1621 ttcaggacat ccagaaagga ctggacaatg ttgaatgcaa tgaatgaaac caccagcact
     1681 gagattgtca cggatgcaat tctggccagt aacgccatcc gcgcctggta cctgctccag
     1741 gtggaaacag ccgcctccag aatcgcatcc gaaagggcac cagtcttttc accagttcca
     1801 atcgatacgg caatgtcgtc gggaagtgac gggtgaagcg ccgccgaaag ggttgcaccc
     1861 ctggcgacca gaagcgccgt ggccttccac gcatccgacg tctgtcgatc ctgcgcggca
     1921 atcgtcccga gtttcagtga ctgggaaatg ccaagtccgg cctgaagtgc caggccgatt
     1981 gtctgaaggc tcgcgaaaag cgccctttcc cttattgcgg cacctacgag aggaacatgc
     2041 cgggcgattc gacggagggc gtctgaagcc cttgggttca aggttgcaaa ccagaatgag
     2101 ccgatgattc cggcaattgc cagaaagggc agagcggtct ttgcgacccc ggccagatat
     2161 cctccgggcc cgcaactgac caggcgtgga agcggaagca gcaggcacgc ggaaagtgcc
     2221 aggaacgccg gataggcaaa cgatgtcaga atgcggcggc gggtttcggc ctgaagcttc
     2281 agggtgcgga ccatcgagtc aagcatgtcg gggatcgttc cggtgtgttc ggccgcacca
     2341 atgaggtcag gggccgttcc cggaaatatc tgcggcgcaa ggcgtgccgc atcggcaagg
     2401 gtaagcccgg attccaggcc ggctgccacg cggttgagcg caatccccgt acggccgcca
     2461 gacaggccgc ccagggactt gaaggcggca accacgggga ttccggcgag aagctggcgt
     2521 tgaaggcttt gaagggtcag cgcccgcttc gcaattgcaa tcggtcgacc ggaaaaggtc
     2581 ttgcccaggc aggcaaaagc ctgaaccacg ccggcctgca gtcccgggcg ttcccttctg
     2641 cctggtgaaa cgatgttttg cgccatcggc aataccttcc tgcaagaagg gcacttcttt
     2701 gacgatccgg gtccggcgtc ataccatcag tacgtcgccg tcgaaagtgg acggacaatt
     2761 ataccccgca ggtgttatga cgcgaatcgc tttgattgca gtcggaacgg tggtgcttgc
     2821 aacttctgcc tgttcggtgc cgacccgtgt ccagaagctt gcactgcatg cctccgcgcc
     2881 cgggcttgcg ggcgagtcag gcccgtttgc agtccttgac ccgggaacca ggccggaatc
     2941 gcgtgattca gccacggatg gcggccggaa tccagaaaag gacaagccgg cggccagccg
     3001 ccaggcatcc ggtggcgcgt cgcaacgtgc ggccgccccg acgcccgctg ttcgacagcc
     3061 cgccccggcc aggacggccg tgttgatggt tccggcatcg ggagatgcca gggcaaggct
     3121 ggccaggtcg gctcgcaggc tggtcg
//
LOCUS       DAOPPA010000390         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33601_length_3142_cov_8.383220, whole genome shotgun sequence.
ACCESSION   DAOPPA010000390 DAOPPA010000000
VERSION     DAOPPA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33601_length_3142_cov_8.383220"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKH54_11040"
     CDS             <1..668
                     /locus_tag="PKH54_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOD00472.1"
                     /translation="WSHKMPRTASGNKRAVLYARVSTTNHGQDPEVQLQPLRRVAGER
                     GWTIVTECVDLGVSGTTGTRPELDRLMSLINSGKIDVVAVARFDRLARSVQHLLAFLE
                     TCRQHHVDFVSISEAVDTTTPVGRMVFTFLGAVAEFERALIVERVKAGVAQAKAEGKH
                     CGRPRQNMDTRAARVLLEQGHPLSDVARMLNLSRTSLRRRLEEESGPVHKSPDATTSE
                     TPV"
     gene            complement(730..1590)
                     /locus_tag="PKH54_11045"
     CDS             complement(730..1590)
                     /locus_tag="PKH54_11045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD00473.1"
                     /translation="MSSVVLEEPDIRTGDCIDVLKKVPDGSVNLIVTSPPYGAQRKDT
                     YGGIPPDQYVDWFLPRAVEFKRTLAADGSFILNIKEHADSGQRHLYVHKLVIALVEQQ
                     GWRLVDEFIWHKKNSSPGKWPNRFRDAWEHVFHFTKNPGFKFRQDQVKIQAAASTLNR
                     AKHLGVRDFEMTAPETGSGLKRCVANCVGRELVYPSNVLHLSAETGNQGHSAAYPVAL
                     PEFFVKLMSAPGDLVMDPFAGSGTTGVVCRMHGRRFLGIDIHKENCDLCRKRWEQTPP
                     SENDEEAAGQ"
     gene            2147..2374
                     /locus_tag="PKH54_11050"
     CDS             2147..2374
                     /locus_tag="PKH54_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD00474.1"
                     /translation="MKSSSPKQADSQLRMISADELAAVLNLSVDSVYRLVRRGILPAF
                     RVGRTVRFDLDKVAAALAVRTPEDMGGPDDK"
     gene            2479..>3142
                     /locus_tag="PKH54_11055"
     CDS             2479..>3142
                     /locus_tag="PKH54_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00475.1"
                     /translation="MNNRQTDIKGKRNVRGSVYPGAAPSPDVNESEARIWETVHDLIE
                     LARQPVGLEDVMNWNPTPDVMPDLTQMLEKEAARAMRDPKGRSSLVASAKRNIEQAYM
                     ISKVRNPRVLARFAVEMAYDYHYRLNVSATLDGTWVRRWKNGHDWINCYPGYPEQGLP
                     IRQDELAMLGWSTARYAAEKLPADNIVEPYEGLKPTFGWAIDPTAYSGDADHRFRSKP
                     ITD"
BASE COUNT          680 a          873 c          893 g          696 t
ORIGIN      
        1 gatggagtca caagatgccg agaactgctt cggggaataa gcgtgcggtg ctgtacgcac
       61 gcgtcagtac aacaaatcac gggcaggacc cggaagttca acttcagcct ctgcggcggg
      121 ttgccggtga acgtggatgg accatcgtaa cggaatgcgt tgatcttggt gtgtccggca
      181 cgaccgggac ccgtcccgaa cttgaccggc tgatgtccct gatcaattcc ggcaagatcg
      241 atgtcgtcgc tgtggcaagg ttcgaccgat tggcccggtc ggtccagcac ttgctggcct
      301 tcctggagac ttgccgacag catcatgttg atttcgtcag catttccgag gccgtcgaca
      361 cgacgactcc ggtcggaagg atggtcttca ccttcctagg ggccgttgcg gaatttgaac
      421 gggctctcat cgtcgaacgt gtgaaagccg gtgtcgcaca ggcgaaggcg gaaggaaagc
      481 attgcggtcg tccccgacag aacatggata cacgtgccgc ccgagttctc ttggaacagg
      541 ggcacccgtt aagcgatgtc gccaggatgc tgaacctttc ccgaaccagc ctccggcgac
      601 gtctggagga agaatccggg cctgtccata agtcgcctga tgcgaccaca tccgaaacgc
      661 cggtgtaggg ccgtccggaa gtcgttgccg aaacggcctc acggcccgac cgaaacctga
      721 tgattctggt cactgtcctg cggcctcttc atcgttttca gacggtggtg tctgttccca
      781 gcgcttccgg cataaatcac agttttcctt gtgaatatcg atgccgagga atcttcgacc
      841 gtgcattcga caaacaacgc cggttgttcc agatccggcg aacggatcca tcacaaggtc
      901 cccaggggct gacatcagct tcacgaaaaa ttccggtaat gctaccgggt acgcggcact
      961 gtggccctga tttccggttt cagccgacaa atgaagtacg ttgctcgggt atacgagttc
     1021 ccgtccaacg cagttggcga cacaccgctt caggccgctg cctgtttcgg gtgccgtcat
     1081 ctcgaagtct ctgacaccca gatgcttcgc ccgattgagg gttgatgcgg ctgcctgaat
     1141 ctttacctgg tcttgccgga acttgaagcc tggatttttc gtgaaatgaa atacgtgttc
     1201 ccaggcatca cggaacctgt tcggccattt gcccggcgat gaattcttct tgtgccagat
     1261 gaactcatcc acgagacgcc agccctgctg ttccaccaga gcaatgacga gtttgtgcac
     1321 gtacagatgc cgctgcccgc tatcggcatg ttccttgatg ttaagtatga acgatccgtc
     1381 agcggccagg gttcttttga actccacagc cctcggcagg aaccagtcca catactggtc
     1441 tggcgggatt ccgccatacg tgtctttcct ttgcgcgcca taaggtggcg acgtcacgat
     1501 caggttgacg ctaccgtcag gtaccttctt cagcacatcg atacagtcgc cggttctgat
     1561 atccggctct tcaagaacga ctgaactcat tttcaagccc tgtccgagcc cccaggggtc
     1621 gaggagccca gccgggggcc ggtgaaggac ctggactcgg tccttctacc cgaacttggt
     1681 cttcaatcta tcgccccccc ccttggtgca gaattcggtt gattcaagtg atgaaaggaa
     1741 actggtctgc acgaaagtcg ccgcaggaat ccacgctgaa gtagtcctca aatcaggatg
     1801 cattttgtcc taaattccga tcatgcatcc tgccgaagtc gaaatcccag gatccccccc
     1861 cgataacggt gatttcgttt gttgccgaaa ggacggcaaa ccccttgtga caaaaggtga
     1921 ttaacctggt cacccccccc cgtttaccgt ttcgaatttg tttgttgcgc gacgacatca
     1981 ttgtgcgcgg cgatttagtc gtcgccagga ctgccgccga aagctctttt ccccttcacg
     2041 taattatttg cggctgacgg gtattgtttt cagaaagtga ccgcagccga cggccccggt
     2101 tatttcctta taggacttct tagtagtagg accatggagg tttttgatga aaagcagcag
     2161 cccgaagcaa gccgattcgc agttgcggat gatatccgct gacgaacttg cagcagtcct
     2221 gaacctgagt gtcgacagcg tgtaccggct tgttcgccgg gggattctgc ctgcgttcag
     2281 ggtcggccgg actgtgcggt tcgacctgga caaggtggcg gcggccctgg ctgtgcgcac
     2341 gcccgaagac atgggggggc ccgatgacaa atgattcttg tcagcgcagg cgatgtcagg
     2401 aacgacatca gcatcggaac cgatgttacg acctggacga gatccttgaa cagctgcgaa
     2461 agcagcggga gatgagaaat gaacaatcgt cagacggaca ttaaaggaaa acgcaatgtc
     2521 aggggttctg tttatcctgg ggcagcaccg tcaccagacg tcaacgaatc agaggctcgg
     2581 atctgggaaa ctgttcatga ccttattgaa ttggctaggc agccggtcgg gctggaagac
     2641 gtcatgaact ggaatccgac ccctgacgtc atgcctgacc tgacgcagat gcttgaaaaa
     2701 gaggctgcca gggcgatgcg agacccgaag ggcaggtctt cactggtagc gagcgcgaaa
     2761 cgaaatatcg aacaggcgta tatgatttca aaggtgcgga acccgcgggt cctcgctcgt
     2821 tttgccgttg agatggcgta cgactatcat tatcggctga atgtctcggc gaccctcgac
     2881 ggcacatggg taaggcggtg gaaaaacgga cacgactgga tcaattgtta tcccgggtac
     2941 ccggaacagg ggctgccgat tcgccaggac gaactggcga tgctcggatg gtctacggcc
     3001 agatacgccg ccgaaaaact gcctgctgac aacatcgtcg agccgtacga aggtctgaag
     3061 ccgaccttcg gctgggcgat tgatcctact gcgtattccg gtgatgccga tcaccgattc
     3121 cgctcgaagc cgatcactga tt
//
LOCUS       DAOPPA010000391         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33621_length_3141_cov_4.511017, whole genome shotgun sequence.
ACCESSION   DAOPPA010000391 DAOPPA010000000
VERSION     DAOPPA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33621_length_3141_cov_4.511017"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PKH54_11060"
     CDS             <1..879
                     /locus_tag="PKH54_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00476.1"
                     /translation="IRALLSRKPSSVVAKGLRLVVSFADPAAGASAALGSGGDAAPAP
                     APAILRQPLVLFYSDGTFRIDLDASSRLRPVPPPEPSPQNLTIGLDEALADIPGTGSP
                     AATISFDNGIAVKCTLFSDSAPENVAAFVSLARGLRSTRMTVGPDHGAGPAGDVSDAA
                     GQTPTGQDNGGGSFSSWAKRPWYDGMVLGRALGGAAISFGRTSDPGFMVPDEFAAGVR
                     HDRPGLLTTLPERPEWGNGAVAITRGAIREHDDRGTIIGACDTESTTRLFSDFAAAER
                     EKRQVLVQGITFSRNP"
     gene            1028..1696
                     /gene="deoC"
                     /locus_tag="PKH54_11065"
     CDS             1028..1696
                     /gene="deoC"
                     /locus_tag="PKH54_11065"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOD00477.1"
                     /translation="MDKPSQLAAIIDHTLLKPTASVDDIRVLCEQAVANRFKSVCVNP
                     TYVMQCAYALDGSGIEVCTVVGFPLGATTTIAKAVETTRAIADGATEIDMVVNQGDVL
                     GGHWDDVIDDVRAVCDASGGALVKVIIETCNLNMNAKVGACVAAALGGADFVKTSTGF
                     AASGATAEDISLMRLVVGAGLGVKASGGIRSTDDALTMVAAGANRIGASASIAIVEGF
                     KAIN"
     gene            complement(1794..2681)
                     /gene="lipA"
                     /locus_tag="PKH54_11070"
     CDS             complement(1794..2681)
                     /gene="lipA"
                     /locus_tag="PKH54_11070"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOD00478.1"
                     /translation="MSCERKRLPEWFKVSLPAGRTAAEMHRRLSRLGLHTVCESARCP
                     NQAECWGAGTATVMIMGDVCTRGCRFCNVKTGHPECAPDPEEPGNVARAVADAGLKYV
                     VLTSVDRDDLADGGAGHFAATIRAVKGLSPEIRVEALIPDYRGDRLTTVLESGVDVLA
                     HNVEVVPRMTPKLRDRRASWENSIGVLVEAGAYRRRDGACVLTKSSLMVGVGESDDEI
                     RDAIRLLSEAGVAMITIGQYLQPTARHWPVDRYVEPVVFDSYREFALSLNIPFIASGP
                     LVRSSYRAAELFALNRLQT"
     gene            2862..2963
                     /locus_tag="PKH54_11075"
     CDS             2862..2963
                     /locus_tag="PKH54_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOD00479.1"
                     /translation="MGSVIKKRRKKMRKHKYEKMRKKMRHERRKRGK"
BASE COUNT          613 a          991 c          940 g          597 t
ORIGIN      
        1 attcgagccc tgctttcccg caaaccgtcc tcggtggtcg ccaaggggct caggctcgtg
       61 gtcagctttg cggatcctgc cgcgggtgcc tccgcggcac tgggttcagg cggcgacgcg
      121 gcacccgcgc ctgccccggc catccttcgg cagccactcg tgctgtttta ttccgacggc
      181 actttcagga ttgatctgga tgccagttca cggcttcgcc ctgttccgcc accggagcca
      241 tctccgcaga acctgaccat cgggcttgat gaagcacttg cggacattcc gggcaccgga
      301 tcaccggcgg cgacgatttc attcgacaac ggaatcgccg tgaaatgcac cctgttttcc
      361 gacagcgccc cggagaacgt cgcggcgttc gtttcgcttg cccgcggact gcgcagcaca
      421 aggatgacgg tcgggccgga tcacggcgca ggcccggcag gggacgtctc ggacgctgcc
      481 ggtcagacgc cgaccgggca ggataacggc ggcggctcat tttcttcctg ggccaaacgc
      541 ccctggtacg acggcatggt tctgggacgt gcattgggcg gggccgccat ttcattcgga
      601 cggacatccg atcccggctt catggtgccg gacgaattcg ccgccggggt caggcatgac
      661 cgccccggtt tgctgacgac gctcccggaa cggcccgagt ggggaaacgg ggcagtcgcg
      721 attacccgcg gcgcgattcg cgagcacgac gaccgtggca caatcatcgg cgcctgcgat
      781 acggagtcaa cgaccaggct tttttccgat ttcgccgcag cggaacggga aaaacgccag
      841 gtacttgtcc agggtattac cttttcaaga aacccttaaa attcgggggg tcgcagtgct
      901 ttttgatgta aaggatcttc ttcgccgaaa cgacgtcacc gcgcttcttc cgcacgttac
      961 gcacgacgtt gccgacatcc tgcaggaatt cctccaggat cggcacgact ggccgggctt
     1021 cgcgagaata gacaagccat cccagcttgc ggcgataatc gaccatacgc tcctgaaacc
     1081 gacggcttcg gtcgatgaca tcagggtact gtgcgaacag gccgttgcca atcgattcaa
     1141 atcggtgtgc gtcaatccca cgtacgtgat gcagtgtgcc tacgcccttg acggcagcgg
     1201 aatcgaggta tgcacggtcg tcggtttccc gcttggcgcg acgacgacga ttgccaaggc
     1261 cgtcgagacc acaagggcga ttgccgacgg cgccaccgaa atcgatatgg tggtcaacca
     1321 gggagacgtg ctgggtggcc actgggacga tgtcatcgac gacgtcaggg cggtttgcga
     1381 cgcatctggc ggggcattgg taaaggtcat catcgagacc tgcaacctga acatgaacgc
     1441 caaggttggc gcgtgcgtcg ctgcggcgct cggcggcgcc gacttcgtca agacatcgac
     1501 aggcttcgcg gcatctggag cgaccgccga ggacatctcg ctgatgaggc tggtggtcgg
     1561 cgctggtctc ggcgtgaagg cttccggcgg aattcgcagc accgacgacg ccctgaccat
     1621 ggttgccgcc ggcgccaacc ggattggcgc atcggcatcg atcgcgattg tcgaaggttt
     1681 caaggccatc aattaacgat cctgcttatc ccggggcggg cggatttcca cgcaggcggg
     1741 cgaattctca ttcgccctta cggatcatgg ggcggatcgg gccgggtcgt gcatcaggtt
     1801 tgaagcctgt tcaacgcgaa cagttccgcg gcgcgataag acgacctgac aagcgggccg
     1861 cttgcgatga acggaatatt cagcgaaagt gcgaactcgc ggtaagaatc gaacacgact
     1921 ggttccacat agcgatcgac cggccagtgc cttgcggttg gttgcagata ctggccgatt
     1981 gtgatcatcg cgacgccggc ctcgctcagc agtcgaatcg cgtcacggat ctcgtcgtcc
     2041 gattcgccaa cgccaaccat caatgaagac ttggtcagaa cgcatgcccc gtcccgccga
     2101 cggtatgcgc ctgcctcgac aagcacccca atcgaatttt cccaggacgc cctgcgatcc
     2161 ctcagcttcg gggtcatgcg cggcacgacc tcgacgttgt gcgcaaggac atcgaccccc
     2221 gattccagaa ccgttgtcag ccgatcgccg cggtaatcag gtatcaacgc ctcgacccgt
     2281 atttccgggc taagaccctt tacggcccgg atggtcgcgg cgaagtgccc cgcgccaccg
     2341 tcggcaaggt cgtcccgatc gacggacgta agcacgacgt atttgagacc ggcatcggca
     2401 acggcccggg cgacgttgcc cggctcctcc ggatccggcg cgcattccgg gtgcccggtc
     2461 ttaacattgc agaacctgca accgcgcgtg cacacgtccc ccatgatcat gacggtcgca
     2521 gttccggcgc cccagcactc cgcctggttc ggacagcgcg ctgactcgca gacggtgtgc
     2581 agcccgagac gcgacagccg ccgatgcatt tcggcagcgg ttcggccggc aggcagtgaa
     2641 accttgaacc actcgggaag cctctttctt tcgcaggaca tgggcgcacc atcgaacaat
     2701 cggacgcaat tatgtccaat ccaccatcat tgtccaccgc gaccgccacc agccctccga
     2761 aacggcactt cggtgccatc gggccttgac agattgtacc ggccccggta acctacacgc
     2821 cgttcgtttt ctttgggcgt aaacattacg aggttttcga tatgggcagc gtgatcaaga
     2881 agcggcgtaa gaagatgcgg aagcacaagt acgaaaagat gcgcaagaag atgcgccacg
     2941 aacgccgcaa gcgcggaaag tagtccgcac ccgctgccgc tcgtccattt gtaaaattcc
     3001 ttcaaatttc cattcgacaa ctggcgccgt cttgctagac ttgcgccatg aacacgggac
     3061 gcatactaat acccgtcttg gccgcagtta tcttttcggc gttatcgccg ggggcagctg
     3121 cctacacgga aattccgctg c
//
LOCUS       DAOPPA010000392         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33868_length_3125_cov_3.847557, whole genome shotgun sequence.
ACCESSION   DAOPPA010000392 DAOPPA010000000
VERSION     DAOPPA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33868_length_3125_cov_3.847557"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PKH54_11080"
     CDS             complement(<1..1673)
                     /locus_tag="PKH54_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOD00480.1"
                     /translation="MAKEYKPYVDPKTSMKELTIPPLVLGALMAIILGAANTYLGLKA
                     GMTVAATFPAAVISMAVLRLFKGNILEENIARTTGAVGEALAAGAIFTIPAFIIVGVW
                     DNMNVFSANWWLATALLAVGGLLGVFLMILLRRTFMEDTSLPFPESQACTEIVKAGQG
                     GQSGAASVFAAMGVAGLIEFFKNGNGIPFIGGSARGAFKLGSESKGAFPYFSPEPSPA
                     FLAVGYIIGPKYGAITAAGGVFGWMFLMPIMLFIMASNDAGFGSQLNSLVSAGNWGVL
                     FGGGEGFVGLSSLYGSKIKMIAIGAMIVSAFYTLFKMRNSLVKGIGRSIGGLKGAGAG
                     GEEVLRTDKDINIKLVFLAVALMAVAMFLLYWLLCKSAVVSLVVTVVMAIMAFLFAAV
                     AGFLVSIIGSSSNPISGLTLSTLLIAAGLLVLLGMGGGYEADGKTMTETMRVGVLAVL
                     GVATVVCCVAGVAGDMAQDWKVGHMLGGTPWRMEIGGVIGVLAASLFLVSVISLLHNT
                     TPGGIGSDSLPAPQAGLMATTAKGIISGSLPWELILMGVFMAIGLICVGV"
     gene            complement(1760..>3125)
                     /locus_tag="PKH54_11085"
     CDS             complement(1760..>3125)
                     /locus_tag="PKH54_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00481.1"
                     /translation="DADCPPDSYCDGVVLGVGSCRTRNECVLDSDCVAGWVCESNGLY
                     MVCVEDNPCTWHEDCGYGFRCVYAQPHNRCVLTSQCNIDSECAENERCVPGPNWNVCK
                     SLEVGCSGDSDCEYGYMCNAAADPDTCAYASTCQSDSDCGTNSTCIDQGNWKECEFDW
                     NPTICNDDRDCGTTEFCDVGLIGVGTCKSRNECDNNWDCPADFVCESNGTYRQCVDGR
                     NCQIHADCELGYRCVPDTPRNICEYANECGADSDCGALEQCVFDGNWTRCSLSVSGVC
                     SADSNCDDDEYCDTELGPLGTCRSRSQCFVDADCGDSGLECRSNGTWNQCMPVIEKNC
                     WFDFQCQEGWSCNDGQCMPVYAGTCSESEGNWTVLVSTCLLVPIGTGYEFVPQNGCDG
                     KIKLRSTGTTNGTFSQTEPGRYDITLSLVYNCTASISLGSIMTMDCPLEGCSSIQMIR
                     GSDK"
BASE COUNT          700 a          988 c          857 g          580 t
ORIGIN      
        1 acgccgacgc agataaggcc gatcgccatg aacacgccca tcaggatcag ttcccacggg
       61 agactgccgg aaatgatgcc cttggcggtg gttgccatca gaccggcctg cggggccggc
      121 agtgaatcgc tgccgattcc gccgggggtc gtgttgtgca gcagggaaat gacggaaacc
      181 aggaacagcg aagcagcgag cacgccgatt acgccgccga tctccatgcg ccacggagtt
      241 ccgccgagca tgtgcccgac cttccagtcc tgggccatgt cgccggcgac accggccacg
      301 cagcatacga cggtcgcgac gccaagaacg gcaaggacgc cgacccgcat cgtttccgtc
      361 atcgtcttgc catcggcctc gtatccgcca cccatgccga gcaggaccag gagtcccgcc
      421 gcaatcagca gcgtcgaaag ggtcaggccg gaaatcggat tcgaggatga accgataatc
      481 gagaccagga atccggcaac ggcggcgaaa aggaacgcca tgatggccat gacgacggtc
      541 acgaccaggg aaaccaccgc gctcttgcac agcagccagt acagaaggaa catcgcaacg
      601 gccatcagcg cgacagccag gaagaccagc tttatgttga tgtccttgtc ggttcgcagc
      661 acttcctcgc cgcctgcacc ggcgcccttc aagccgccga ttgaccgccc gatacccttg
      721 accaggctgt tacgcatctt gaagagcgta tagaaggcgc tgacaatcat cgcgccgatc
      781 gcgatcatct tgatcttgct gccgtacagc gaggaaaggc cgacaaagcc ttccccgccg
      841 ccaaaaagga cgccccagtt tccagccgac acgaggctgt tcaactgact gccgaaaccg
      901 gcatcgttgg aggccatgat gaacagcatt atcggcatca ggaacatcca gccgaagaca
      961 ccgccggcgg ccgtgatcgc gccgtacttc ggtccgatga tgtatccgac cgcaaggaag
     1021 gcgggcgaag gctcggggga gaaatacggg aaggccccct ttgattccga gccgagcttg
     1081 aaggcgccgc gggccgaacc gccgatgaaa ggaatgccgt tgccgttctt gaagaattcg
     1141 atcagccccg cgacacccat cgcggcaaag acgctggcgg cgccggactg gccgccctgc
     1201 cccgccttca cgatttcggt gcaggcctgg ctctccggga acggcaggct cgtgtcttcc
     1261 atgaacgtcc tgcgcaacag gatcatcagg aaaacgccaa gcaggccgcc gacggccagg
     1321 agtgccgttg caagccacca gttggcggaa aacacgttca tgttgtccca gacgccgacg
     1381 atgatgaatg ccggaatcgt gaagatcgcg cccgctgcca gtgcctcgcc gaccgcgccg
     1441 gtggttctgg cgatattctc ttcaaggatg tttcccttga acagccgcag cacggccatt
     1501 gaaatgacgg cagcggggaa cgtggccgcc accgtcatgc cggccttcag gcccaggtag
     1561 gtattggccg cgcccaggat gatggccatc agggcaccaa gaaccagcgg cggaatggtc
     1621 agttccttca tgctggtctt cgggtcaacg taaggcttgt attccttcgc cataaaggtc
     1681 tccttgtccg aagcgatgaa tcggaaatcc ttgcagaacg gaaggtaacg cggcaactgc
     1741 ggaaatgtcc agtcaaaaac tacttgtcgg aaccacggat catctggata ctggagcatc
     1801 cttccaacgg acaatccatc gtcatgatgc tgccaagcga aatgctcgcg gtgcagttgt
     1861 acacaaggga cagggtaatg tcgtaacggc ccggttcggt ctgcgagaat gttccgttcg
     1921 tggttccagt cgaccgcaac ttgatcttgc cgtcgcatcc gttctggggt acgaactcat
     1981 aacccgtacc gattggcacc agcaggcacg tcgaaacaag gaccgtccag tttccctcgg
     2041 actcgctgca tgtgcccgca taaaccggca tgcactgacc gtcgttgcag gaccagcctt
     2101 cctgacactg gaaatcgaac cagcagtttt tctcgatgac cggcatgcac tggttccatg
     2161 tcccgttcga cctgcactcc agccccgaat cgccgcaatc ggcatccaca aagcattgac
     2221 tgcggctgcg gcaggtcccg agcggcccaa gttccgtatc gcagtactcg tcatcatcac
     2281 aattggagtc agcgctgcat acaccggaaa cggacaacga acaccttgtc cagtttccgt
     2341 caaacacgca ctgttcaaga gcgccgcaat ccgaatcggc cccgcactcg ttcgcatact
     2401 cacaaatgtt cctgggtgtg tccggaacgc aacggtagcc aagttcgcaa tcggcatgaa
     2461 tctggcaatt tcgaccgtca acgcactgtc gataggtgcc gttggactca cagacgaagt
     2521 cagccggaca atcccagttg ttgtcgcatt cgttgcgtga cttgcacgtg cccacaccga
     2581 taagtccgac gtcacaaaac tccgttgtcc cgcaatcacg atcgtcgtta catatcgtcg
     2641 gattccaatc gaactcgcac tccttccagt tcccctggtc aatgcaggtc gaattggttc
     2701 cgcagtcgga atcggactga catgtgcttg cgtacgcgca cgtgtccgga tccgcggcgg
     2761 cattgcacat gtacccgtac tcacaatcgg aatcgccgga acaacccact tcaagtgact
     2821 tgcagacgtt ccagttcggc cccggaacgc agcgttcatt ctcggcacat tcgctgtcga
     2881 tattgcactg gctggtcaaa acgcaccggt tatgcggctg ggcatacacg cacctgaatc
     2941 catatccgca gtcttcgtgc catgtacagg ggttgtcttc cacgcatacc atgtaaaggc
     3001 cgtttgattc gcatacccac ccggcaacgc agtccgaatc caagacgcat tcgttcctgg
     3061 tcctgcacga tccgaccccc agaaccactc cgtcacaata gctgtccggc ggacaatcgg
     3121 catcc
//
LOCUS       DAOPPA010000393         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_33933_length_3120_cov_3.661664, whole genome shotgun sequence.
ACCESSION   DAOPPA010000393 DAOPPA010000000
VERSION     DAOPPA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33933_length_3120_cov_3.661664"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKH54_11090"
     CDS             <1..900
                     /locus_tag="PKH54_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00482.1"
                     /translation="DPFTYTTTNVMACDGSTPAATGTAGGLGDVGPHAAGYVDFVSDD
                     VRVYYPAECGSVRRPGIPGTYPVLIIVPEAQDTNFPFINFDYLGQMLATWGIVTVSLD
                     AKLVEEDPQGHPKKVFATAPKYLNADLGLLHSAMTGVQTGGTFAWMVFGSGSDTLNLL
                     KDVDKESTIYNKAVATIAMAPSIKLNKNGAQYFMALLGPKDTIANSSYANDSYSAFDS
                     PKWKVQVVGGNHSLFTDHQMYYGGGMMPIMDGEPEIMRKEQLYLSVSMILPFLQLAFG
                     LDQPFEDQVKTGFSDGKLVVSKG"
     gene            943..2625
                     /gene="ettA"
                     /locus_tag="PKH54_11095"
     CDS             943..2625
                     /gene="ettA"
                     /locus_tag="PKH54_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOD00483.1"
                     /translation="MADDPQKVIYSMMGVGKVHGQKTVLKDIWLSYFYGAKIGVIGAN
                     GSGKSSLLKIMAGVDTEFLGQTACAPGFSIGYLEQEPKLDPQKTVIDVVKEGAAPIVE
                     LLAEYDRINERFGEPMSDDEMDALMKRQGEVQDKLDAVGAWELDSRLELAMDALRCPP
                     ADTPVNILSGGEIRRVALCRLLLQNPDILLLDEPTNHLDAESVHWLEQHLQRYQGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKQERLARETKSESERQKTLAR
                     ELEWIHMSPRGRQAKGKARVTAYEELLAKQPEQMIRELEIYIPAGPRLGDRVIEAGGV
                     SKAFDDRLLIDNLSFLMPPGAIVGIVGPNGAGKTTLFKMITGQEKPDSGTINLGATVR
                     IACADQMRSVLDPDKTVWEVVTGGADRILLGGVEINSRAWVARFNITGADQQKKVGVL
                     SGGERNRVQLAWMLKSEANVLLLDEPTNDLDVNTMRALEDALLNFAGCAMVISHDRWF
                     LDRIATHILAFEGDSSVVWFPGNYSDYERDRRERLGEAADQPHRIRYRSLVR"
     gene            complement(2637..2900)
                     /locus_tag="PKH54_11100"
     CDS             complement(2637..2900)
                     /locus_tag="PKH54_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD00484.1"
                     /translation="MAGYTDTVGSHESNFELSRKRAAAIASWLKAHGVRLPVCSHGFG
                     ETVLAVETPDNTPEEKNRRTIFVISGQAPHGKDFQGEADWSCH"
     gene            complement(2892..3120)
                     /locus_tag="PKH54_11105"
                     /pseudo
     CDS             complement(2892..3120)
                     /locus_tag="PKH54_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          680 a          917 c          969 g          554 t
ORIGIN      
        1 gatccgttca cctacaccac gacaaacgtg atggcctgcg acggcagcac gcccgccgcg
       61 accggaaccg ccggcggact gggcgatgtc ggcccgcatg ccgccggata cgtcgatttc
      121 gtcagcgatg acgtcagggt ctattacccg gccgaatgcg gttcagtccg tcgtccaggg
      181 atcccgggca cctatcccgt cctgataatc gtcccggaag cccaggacac gaatttcccg
      241 ttcatcaatt tcgactacct gggccagatg ctggcgacat ggggaatcgt tacggtaagc
      301 cttgacgcaa agctggtcga ggaggatccg cagggacacc cgaagaaggt gttcgccacc
      361 gcaccgaagt acctgaacgc cgacctgggc ctgcttcatt ccgcaatgac cggcgtgcag
      421 accggcggca ctttcgcctg gatggtgttc ggcagcggct ccgacacgct gaacctgctc
      481 aaggacgttg acaaggaatc gaccatttac aacaaggccg ttgccaccat cgcgatggcg
      541 ccgtccatta agctcaacaa gaacggggcc cagtacttca tggcgctgct gggaccgaag
      601 gacaccatcg ccaacagcag ctatgccaac gattcgtatt cggcattcga ctcgcccaag
      661 tggaaggttc aagtcgtcgg cggcaatcac agcctcttta ccgatcacca gatgtattac
      721 ggcggcggaa tgatgccgat aatggacggc gagcccgaaa tcatgcgcaa ggaacagctc
      781 tacctgtcgg tgagcatgat tcttccgttc ctgcagcttg cgttcggcct tgaccagccg
      841 ttcgaggatc aggtcaagac aggcttttcc gacggcaaac tggtcgtttc gaagggctag
      901 ccgaccgcgg ctcatcttca aatcaaaccg aggttcaggg aaatggcgga cgatccgcag
      961 aaagtaatct attcgatgat gggcgtcggc aaggttcacg gccagaaaac cgtgctgaag
     1021 gacatctggc tgtcgtactt ctatggggcc aagatcggcg taatcggcgc caacggatcg
     1081 ggcaagtcgt ccctgctgaa gataatggcc ggcgtcgata ccgaatttct gggccagacg
     1141 gcgtgtgctc ccgggttttc cataggttac cttgaacagg agccgaagtt ggacccgcag
     1201 aagacggtta tcgacgtcgt gaaggaaggc gcggcgccaa tcgtcgaact gcttgcggaa
     1261 tacgatcgaa tcaacgagcg cttcggcgag ccgatgtccg acgacgaaat ggacgccctg
     1321 atgaagcgcc agggcgaagt acaggacaag cttgacgccg ttggcgcgtg ggaactggac
     1381 agccgcctgg aactcgccat ggacgcgctg cgctgcccgc cggcggatac ccccgtcaac
     1441 atcctgtccg ggggcgaaat ccgccgtgtc gcgctttgcc gcctgctgct gcagaatccg
     1501 gacatacttc tgctggacga gccgaccaac cacctggacg cggaatcggt tcactggctc
     1561 gaacagcacc ttcagcgtta ccagggaacg gtaatcgcgg tcacccacga ccggtacttc
     1621 cttgacaacg tcgccggatg gattcttgaa cttgaccgcg gcgagggtat cccgtggaag
     1681 ggcaattaca gttcctggct tgagcagaag caggaaaggc tggcgcgcga gactaaatcc
     1741 gaaagcgaac gccagaagac gctggcgcgc gagctcgaat ggattcacat gtcgccacgc
     1801 gggcggcagg ccaaggggaa ggccagggtc acagcttacg aggaactgct tgcaaagcag
     1861 cccgagcaga tgatccggga acttgaaata tacataccgg ccggacctcg gcttggcgac
     1921 cgggttatcg aagctggcgg cgtatcaaag gctttcgacg acagactcct tatcgacaac
     1981 ctgagcttcc ttatgccgcc cggggccatc gtcgggattg tcggaccgaa cggagccgga
     2041 aagacaacgc tgttcaagat gatcaccgga caggagaagc cggattccgg gacgatcaac
     2101 cttggcgcca cagtcaggat tgcgtgcgcc gaccagatga gaagcgtcct ggatccagac
     2161 aagacggtct gggaagtggt gacgggcggg gccgaccgca tccttcttgg cggtgttgaa
     2221 atcaactcgc gggcgtgggt tgcaagattc aacatcaccg gcgcggatca gcagaagaag
     2281 gtcggcgtgc tgtcgggcgg cgagcgcaac cgcgtgcagc ttgcatggat gctgaaatcg
     2341 gaagcaaacg tcctgctgct ggacgaaccg accaacgacc ttgacgtcaa cacgatgcgg
     2401 gcactggaag acgctctgct gaacttcgcc gggtgcgcga tggtgatttc ccacgaccgg
     2461 tggttcctgg acaggattgc aacccatatc ctggcgttcg agggcgacag cagcgtcgtc
     2521 tggtttcctg gcaactactc ggattacgaa agggatcgcc gggaaagact tggcgaggcc
     2581 gcagatcagc cgcacaggat caggtacaga tcactggtca ggtgacctgg ccgccctcaa
     2641 tgacaagacc agtcggcttc accctggaaa tccttgccgt gcggggcctg gcccgatatc
     2701 acgaatatgg tgcgacggtt cttttcctcc ggggtgttgt ccggcgtctc gaccgcaagg
     2761 acggtttcgc cgaaaccgtg cgaacacacg ggaagcctga ccccatgcgc cttcagccag
     2821 gacgcgatcg cggcggcgcg cttcctggaa agctcgaaat tcgattcgtg ggagccgacg
     2881 gtatcggtgt agccggccac gaacagctgg ccgatggatt gctcagtgag cacgccatag
     2941 atgatcagga ccacgctggg cggcatgacc atgccaaggt ccccgccggc ggccacggct
     3001 ccggcggcca ggcggtcact gtagccgaag cgcttcattt ccggaatggc cacagtggcc
     3061 atggttgcgg cagtggccgg gctggacccg caaaccgccc caaaggccgt acagctggtg
//
LOCUS       DAOPPA010000394         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34063_length_3111_cov_4.109620, whole genome shotgun sequence.
ACCESSION   DAOPPA010000394 DAOPPA010000000
VERSION     DAOPPA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34063_length_3111_cov_4.109620"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKH54_11110"
     CDS             complement(<1..626)
                     /locus_tag="PKH54_11110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866632.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD00485.1"
                     /translation="MESRKGVITQVIGPVVDVRFAPGEIPEINTALAVTNPTINDREN
                     NLVLEVAQHTGESTARCIAMDTTDGLVRGMAAIDTGAPIKIPVGPETLGRIMNVVGLP
                     VDEGGPIDGKTFYPIHREPPSFLDQDVAVKQMETGIKVIDLLEPYPRGGKVGLFGGAG
                     VGKTVVIMELIHNIATHHGGVSVFGGVGERTREGNDLWLEMKESGVIER"
     gene            complement(676..1554)
                     /gene="atpG"
                     /locus_tag="PKH54_11115"
     CDS             complement(676..1554)
                     /gene="atpG"
                     /locus_tag="PKH54_11115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOD00486.1"
                     /translation="MATLKQIRKRLTGVRNTQKITRAMKLVAAAKLRRAQENMLKAKP
                     YANRMRDLIWDLSEHTSHDAHPLLQIRPEENALMVVITSDRGLCGAFNSNINRRAERY
                     LKSGEGGHARVELALMGKKGRDYFRRRGFPIAAEYMDVLTDPNFNHVAEIGGELVAKF
                     LDGKLDAVYVVYNEFKSALSQQVVVERLLPIVPEVSQQDGPGQTIQGLEFIFEPSAGA
                     VLDNVLPMYFNIRLYFAVLESLAAEMGARMTAMESATQNASELISKLSLMYNKARQAA
                     ITTEMLEIVSGAEAMK"
     gene            complement(1595..>3111)
                     /gene="atpA"
                     /locus_tag="PKH54_11120"
     CDS             complement(1595..>3111)
                     /gene="atpA"
                     /locus_tag="PKH54_11120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD00487.1"
                     /translation="EVTEILKKEIREFGRKVEVRETGTVITVGDGIARVYGLSGAMAG
                     ELLDFPHGVQGLVLNLEESNVGVALLGEVNKIREGDECRRTGRIASVPVGPAVRGRVL
                     NALGQPVDGLGPIESTELRPIDVKAPGIVARQPVKEPLQTGLKSIDAMTPIGRGQREL
                     IIGDRQTGKTAVAIDTIINQKPQREGDQPVWCIYVAIGQKRSTVAQVVDKLRQHGAMD
                     YTTVVMATASETAPMQYIAPFAGCAMGEYFRDNGQHALIIYDDLSKHAVAYRQLSLLL
                     RRPPGREAYPGDVFYLHSRLLERAAKMSAELGGGSLTALPIIETQAGDVSAYIPTNVI
                     SITDGQIYLESDLFYKGFRPAINVGLSVSRVGGNAQIKAMKKVAGTLRLELAQYRQMA
                     SFAQFATDLDKATKRQLDKGARMMELLKQRQYRPEAVERQIVSLFAASADEGFLMDVA
                     IPDVGRFEHDLLDFIDARHSDIFTDIREKKVLDDAIRARLVAAINEFKALFNSK"
BASE COUNT          539 a          975 c          930 g          667 t
ORIGIN      
        1 cgttcgatga cgcccgattc cttcatctca agccacaggt cgttaccttc gcgggtacgt
       61 tcgccgacgc cgccgaatac cgagacgccg ccgtgatgcg ttgcgatgtt gtggatgagt
      121 tccatgatga cgacggtctt gccgacgccc gcgcccccga aaagtccgac cttgccgcca
      181 cgcgggtacg gttccagcag gtcgatgacc ttgatgccgg tttccatctg ctttacggcg
      241 acatcctgat ccaggaatga cgggggttcc ctgtggattg gataaaacgt ctttccatcg
      301 atcggtccgc cctcgtcaac cggcagcccg acgacgttca tgattctccc aagcgtttcg
      361 ggcccgacgg ggatcttgat cggagcaccg gtgtcgatcg ccgccatccc gcgcacgagt
      421 ccgtcggtcg tgtccatcgc gatgcagcgg gccgtcgatt cgccggtgtg ctgcgcgact
      481 tccagaacca ggttgttttc ccgatcgttg atggtcgggt tcgtgaccgc cagggccgtg
      541 ttgatttccg gaatttctcc gggcgcgaag cggacatcga caaccggccc gataacctgg
      601 gtgattacgc ctttcctgga ttccataact gcaaagcctc caagggcgga cgccgccgcc
      661 cgtgcaccat gacccttact tcatcgcctc ggcgccggaa acgatttcca gcatttcggt
      721 ggtaatcgcg gcctgcctgg ccttgttgta catcagggac agctttgaaa tcagctcgct
      781 tgcgttttgc gtggccgatt ccattgccgt catgcgggca cccatttcgg cggccagcga
      841 ttcgagcacc gcgaagtaaa gcctgatgtt gaaatacatc ggcagaacat tgtcaagaac
      901 cgcgcccgcc gacggctcaa atatgaattc aagcccctga atcgtctggc ctggcccgtc
      961 ctgctgcgaa acctcgggca cgattggcag cagccgctcg accaccacct gctgggaaag
     1021 agccgacttg aattcgttat agaccacata gacggcgtcc agcttgccgt ccaggaactt
     1081 cgcgaccagt tcgccgccaa tctcggcgac gtgattgaag ttcgggtcgg taaggacgtc
     1141 catgtattcg gccgcgatcg ggaagcccct tcgtcggaag tagtcgcgcc ccttctttcc
     1201 catcagcgcc agttcaactc tggcgtggcc gccttccccg ctcttcaggt acctttcggc
     1261 acgtcgattg atgttgctgt tgaacgcccc gcacaggccg cgatcggatg tgatcacgac
     1321 cataagggca ttctcctcgg gtcggatctg cagaagcggg tgggcgtcgt gcgaagtgtg
     1381 ttccgacaga tcccatatga ggtcacgcat gcggttggca taaggcttgg ccttcagcat
     1441 gttttcctgg gcccgccgca gcttggccgc ggccaccagt ttcattgccc tggtgatctt
     1501 ctgcgtgttt cgaacgccgg tcaggcgctt gcgaatctgt ttcagcgtgg ccatgcgaac
     1561 ctgcctgcgg ccccttcagg cgccgcccct tccatcactt cgagttgaaa agcgccttga
     1621 attcgttgat tgccgcgacc aggcgtgccc ggatggcatc gtccagaacc ttcttttcac
     1681 ggatgtcggt gaagatgtcc gaatgcctgg catcgatgaa atccagcagg tcgtgttcga
     1741 atcggccgac gtccggaatc gcgacgtcca tcaggaagcc ttcgtcggcc gatgcggcga
     1801 aaaggctgac gatctggcgt tcaaccgcct cgggacggta ctggcgctgc ttgagaagct
     1861 ccatcatgcg ggcgcccttg tccagctgac gcttcgtggc cttgtcaaga tccgtcgcga
     1921 actgggcgaa cgaagccatc tggcggtact gggccagttc caggcgcaag gtcccggcga
     1981 ccttcttcat cgccttgatc tgcgcgttcc cgccgactcg ggaaaccgaa agaccgacgt
     2041 tgatggccgg ccggaatccc ttgtagaaaa ggtcggattc aagataaatc tggccgtcgg
     2101 taatcgaaat gacgttcgtc gggatatagg cggaaacgtc cccggcctgg gtctcgatga
     2161 tcggcagcgc cgtcagggaa ccgccaccca gttccgcgct catcttcgcg gcgcgttcca
     2221 gcagcctgga atggagatag aaaacgtcgc ccggataggc ctcgcgtccc ggcggacggc
     2281 gaagcagcag ggaaagctgc ctgtaggcga cggcgtgttt cgacaggtcg tcatagataa
     2341 taagggcgtg ctgtccgttg tcccggaaat actcgcccat cgcgcacccg gcaaacgggg
     2401 cgatatactg catcggggct gtttccgacg cggttgccat cacgaccgtc gtgtagtcca
     2461 tcgcgccgtg ctgtcgcagt ttgtcaacca cctgggcgac cgttgaacgc ttctggccga
     2521 tggcgacgta gatgcaccat accggctggt cgccttcgcg ctggggcttc tggttgatga
     2581 tcgtgtcgat ggcgacggcg gtctttcccg tctggcggtc gccgatgatc agttcccgct
     2641 ggccacgtcc aatcggggtc atcgcgtcga tcgacttcag accggtttga agcggttcct
     2701 tgaccggctg gcgcgcgacg atgcccggag ccttgacgtc gatcggccgc agttcggtcg
     2761 attcaatcgg cccgaggcca tccaccggct gcccgagcgc gttcaggacg cgtcccctga
     2821 ccgcggggcc gaccggaacg ctggcgatgc ggccggttcg gcggcattcg tcgccttcgc
     2881 ggatcttgtt gacttcgcca aggagcgcga cgccgacgtt cgactcctcg aggttcagga
     2941 ccagtccctg cacgccgtgc gggaagtcca ggagttcgcc cgccatggcg cccgaaaggc
     3001 cataaacacg cgcgatgccg tcgccgacgg ttatgacggt tccggtctcc ctgacctcga
     3061 ccttgcggcc gaattcgcgg atttcctttt tcaggatctc tgttacttcc t
//
LOCUS       DAOPPA010000395         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34255_length_3098_cov_4.333224, whole genome shotgun sequence.
ACCESSION   DAOPPA010000395 DAOPPA010000000
VERSION     DAOPPA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34255_length_3098_cov_4.333224"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PKH54_11125"
     CDS             <1..892
                     /locus_tag="PKH54_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00488.1"
                     /translation="RQKWGQVTFTVAQMPDQTYEGIAFDSNNFNSDTILYGARLTMPF
                     NRLKLDAAIWGIHDSQVVGRTMDLMAITANLSGDWRWIKFGLDLGLQYGTTEGRAGGD
                     NETTLAWALQAYMNMDRRLQGVKDLNFLLDLNVLAISADDDWDGNTRNGAWLYSGKSR
                     SRTLILTEDELRDRGGNIDELVSDRRNADKGKFWLNRAGLTVADVSFGLLHKNFFKPM
                     VTIGAGWALNDRNALGANFVGLEADLHLEFLYKKYLSVDIAGTTFLPGKAAAAFVNKG
                     PFPGAVDPVYQLEASVMFFF"
     gene            990..>3098
                     /locus_tag="PKH54_11130"
     CDS             990..>3098
                     /locus_tag="PKH54_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD00489.1"
                     /translation="MSATRPALLALMTAGLLLCSRDSAAQSAQAEPPAGSDASTRVLF
                     LNSYHQGYEWSDSITTGIMSVFSQIAPNVGIYIEYLDSKHFPFTAERQQATTKALQEK
                     FKPDFFDLIITSDDRAFTFMKESRDALFPETPVVFTGVNFLDEAVTPLPPGFAGVVES
                     FDYKGIAAMARTLFPERRKAWIIVDNSITGDNIVKAAAALFPIDDLSVDFLSGRTLSH
                     AELLTRLSMIGDDSIVIIASWQRDRTNRYLTVTEAFESIVSATSAPVFVVLRTPPSIG
                     MAGGSAVSGIHQGRMAARIALEILGGKPPAEIPVMRDSVHDRMLNKPVLEDRWNIRWN
                     ELPPEIRSIPATFPLERNYHLPRELVLTTQEIEFIRSRPEIHAVLRDAPPLMIPGSPP
                     SGMVVDLLTEISALNGLAITIDELKHFQDGNPQSMDSTEVQNRADGRGNSRVIHILAA
                     KGSNLDESWILTSPYTRLHTVIFSRDDQAYIGGIDSLAGLKAGVVSGSGIEAILKDEH
                     PDVRVVSYQSAREGILALENGEIDSWVGFLSTGLWLKKELGLAGISVAAPTGLPPQPI
                     SFAIPKSEKILADILSKSIQALPPDHGEMLKDGYMDRLIIRQGWSTDSVLKMLLAVAV
                     AFLVVVTVFAVRIRASRQETFKREQNLRVTLDSIADAVIVTDPGLNITRMNPVAEALT
                     GWGRNDAIGRHVAAVMRLADP"
BASE COUNT          660 a          928 c          874 g          636 t
ORIGIN      
        1 caggcagaaa tggggccagg tgactttcac ggtcgcccag atgccggatc agacgtacga
       61 aggaatcgcc ttcgacagca acaacttcaa ttccgacaca atcctttacg gggcccgcct
      121 gacgatgccg ttcaacaggc tgaagcttga cgccgcgatc tggggcattc acgacagcca
      181 ggtggtcggc cgcacgatgg atctgatggc catcacggcc aacctgtccg gcgactggcg
      241 gtggatcaag ttcggtctgg atcttggcct tcagtacggc acgactgaag gaagggccgg
      301 cggcgacaat gaaacgaccc ttgcctgggc acttcaggcg tacatgaaca tggatcgcag
      361 gcttcagggc gtgaaggatc tcaacttcct tctcgacctg aacgttcttg ccatcagcgc
      421 cgacgacgac tgggacggca acacgcgcaa cggcgcatgg ctctactcgg gaaagagccg
      481 cagcaggaca ctgatcctga cggaagacga actccgcgat cgcggcggaa acatcgatga
      541 actggtttca gaccgaagga acgcggacaa gggaaagttc tggctgaacc gcgccggcct
      601 tacggtcgcc gatgtctcat tcgggcttct tcacaagaac ttcttcaagc cgatggtcac
      661 gattggcgcc ggctgggcgc tgaacgaccg caacgcgctg ggcgccaatt tcgtgggcct
      721 ggaagccgac ctgcacctgg aattcctgta caagaagtac ctgtcggtcg atatcgccgg
      781 aaccactttc cttccgggca aggccgccgc ggccttcgtc aacaagggcc ccttcccggg
      841 cgcggtcgat cccgtttatc agcttgaagc ttccgttatg ttcttcttct gattacacgg
      901 ttaaggcctt ccagccagtt actttcaccg cgtaaacgtg tatcatcggt tcatccagaa
      961 gacgtgcggt gcacggaatg tctcgattgg tgtctgcaac cagaccggcc ctgcttgccc
     1021 tcatgacagc cgggcttctg ctttgcagcc gggattcggc cgcacaaagt gcccaggcag
     1081 agccgccggc cggaagcgac gcatcaacgc gggtactgtt tctgaactcc taccaccagg
     1141 gctacgagtg gtccgactca atcacgaccg gcatcatgtc ggtcttttcg cagattgcgc
     1201 ccaacgtcgg gatatatatt gagtatcttg actcgaagca cttccctttc acggcggaaa
     1261 gacagcaggc caccacgaag gctcttcagg aaaaattcaa acccgacttc ttcgacctga
     1321 tcatcacaag cgacgacagg gcattcacct tcatgaagga atcccgcgac gcgctgttcc
     1381 cggaaacccc ggtcgtcttc accggcgtga acttccttga cgaggcggta acgcccctgc
     1441 cgcccgggtt cgccggcgtc gtcgagtctt tcgactacaa ggggattgca gccatggccc
     1501 ggacgctctt cccggagcgc cgcaaagcct ggatcatcgt tgacaactcg atcaccggcg
     1561 acaatatcgt aaaggcggcg gcggccttgt ttccaataga cgacctttcc gtcgatttcc
     1621 tgtccggtcg gacgctttcg cacgccgaac tgctgactcg cctctcgatg atcggcgatg
     1681 acagcatagt gatcatcgca tcatggcaac gggatcgaac caaccggtac ttgacggtga
     1741 ccgaggcttt cgaaagcatt gtatccgcga cttccgcgcc ggttttcgtc gttcttcgca
     1801 cgccgccgtc aatcggcatg gccgggggct cggccgtttc cgggattcat cagggtcgaa
     1861 tggcggccag gattgcgctt gaaatccttg gcggcaagcc gcctgccgaa ataccggtaa
     1921 tgcgcgacag cgttcacgac aggatgttga acaaaccggt tcttgaagac cgctggaaca
     1981 tccgttggaa cgagttgccg ccggaaatca ggtcgatacc tgcaaccttc ccccttgaac
     2041 gaaactatca tcttccgcgt gaacttgtgc tgaccaccca ggaaatcgaa ttcatccggt
     2101 caagacccga aattcacgcc gttcttcgcg acgctccgcc attgatgatt cccggttctc
     2161 cacccagcgg aatggtcgtc gatctgctta ccgaaatctc ggcgctgaat ggattggcaa
     2221 ttacaatcga tgaactgaaa cactttcagg acgggaatcc acagagcatg gattccacgg
     2281 aagttcaaaa ccgcgccgac ggccggggca attccagggt cattcacatc ctggcggcaa
     2341 agggcagtaa cctggatgaa tcctggattc ttaccagtcc gtacacccgt cttcacacgg
     2401 tcatcttttc aagggatgat caggcctaca tcggcggcat tgactccctt gccggactga
     2461 aagcgggtgt ggtttcggga tcgggcatcg aggcaatcct gaaggacgaa cacccggacg
     2521 tccgggtagt gtcctaccag tccgcgcgcg aaggaatcct ggcgcttgag aatggggaga
     2581 tcgattcctg ggttggcttt ctttccacag ggctctggct gaagaaggaa cttggactgg
     2641 cagggatttc tgtcgccgcc ccgaccggcc tcccgccaca gccgataagc tttgcgattc
     2701 ccaaatcaga gaagattctt gccgacatac tttccaagag cattcaggca cttccgcccg
     2761 atcacgggga gatgctgaag gacggctaca tggatcgcct gatcatccgg caggggtggt
     2821 caaccgattc ggttctgaaa atgctccttg cggtcgcggt ggccttcctt gtggtggtca
     2881 ccgtttttgc cgtccgaatc agggcaagcc ggcaggagac attcaagcgc gagcagaacc
     2941 tgcgcgtgac gcttgactcg atcgccgatg ccgtcatcgt gaccgacccc ggcctgaata
     3001 tcaccagaat gaatccggtg gcggaagctc tgaccggttg gggcaggaat gacgcgattg
     3061 gcagacacgt cgccgccgtc atgcgacttg ccgatccc
//
LOCUS       DAOPPA010000396         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34279_length_3097_cov_3.640368, whole genome shotgun sequence.
ACCESSION   DAOPPA010000396 DAOPPA010000000
VERSION     DAOPPA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34279_length_3097_cov_3.640368"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKH54_11135"
     CDS             complement(<1..980)
                     /locus_tag="PKH54_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00490.1"
                     /translation="MTHDQVFRFVDAEGDPESGVIRDPSGRLQASLGDLSLSVMPVGG
                     KPTDSSRVATTVRLEREADGTVKVFSTIRNLSDGPLALDRAVFSTAGFAGEFGLSGDL
                     DQWSRFKMGFNTGSPSGAAPITVPDSSFFLKGLPYSWLPAAVRNMVYFDGTTFSRKVG
                     QFESEWFTALYDSQARRGFAVGFFGVSLNFGRVAIDLGRRLVEIIAFLDGATLEPGQT
                     RELDPLVICFEDDFNLLLQKYARAVADRQQARVNPARIWCSWYSGFYDRVTVKDIREN
                     MSRLSVLKAPVDFIQLDDGYQSVIGDWLVTNSRFPEGLEALACEILQSGF"
     gene            complement(1010..2359)
                     /locus_tag="PKH54_11140"
     CDS             complement(1010..2359)
                     /locus_tag="PKH54_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOD00491.1"
                     /translation="MKSNSKLVVSLVMAVLVAAPVLLPSVALAGGYSNLDFGGRRMGM
                     FAVVGKPDDVTAVFHNPAGLTLQNGTQIYFALSTFVLDLGMKLYNSKGDLLPADHEIT
                     PDISIGAIPFIGVATDFGTKRFRAALAIYAPNAYGASLPIDEPTRYHVTQALFLSTRL
                     TASAAYDFNNYISAGVSLSVLYTYLTATRFLNTGMLPSPADVQNDPKYDGRFNSTEAN
                     MKNDIQLDIQGSNWTWAFDIGLLVRPVKSLSIGLVFSSGSKLDLRGNVTAKYRLHIDG
                     REDLLGKTKQTTNMIIPLSIRAGINWAIVPNFEVAADVSYWHYQTNQMQISKLDSPVA
                     GLNGFSDAKNYGNSWNWCLGILYRPIPELDLMAGYQMDYTPNPTSTLSLENPGVDQKG
                     FSVGTRWRINDHWRVSLAYVHNWFNLIDVQDSIGTPPANAKGHGTNNEFAFDFQYRF"
     gene            complement(2401..>3097)
                     /locus_tag="PKH54_11145"
     CDS             complement(2401..>3097)
                     /locus_tag="PKH54_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD00492.1"
                     /translation="MRITPFEDSKLAADMDGLYGLQDHFGLQFAAGVIYDTRDHEYNP
                     VKGIYVESSLRFSPGMDNNLHYGAFGTDARFFVPILGPQLSFATRLMLDMLFGNVPFY
                     EMARAGAFEPIEFIGGRMGLSGIPEGRYHGKIKVGANLELRSQIFPFRIKKYRGMFGT
                     AFFCNIGRVWADYSGNALLDGNSLGLKVAAGGGLRIQFGETVIIRADLAWAKEARDAG
                     SPVGIYLDAYHPF"
BASE COUNT          672 a          919 c          912 g          594 t
ORIGIN      
        1 ttgaaaccgg actggagtat ctcgcatgca agggcttcca ggccttccgg aaagcgcgaa
       61 ttcgtcacaa gccagtcgcc tatgaccgac tggtaaccgt cgtcaagctg gatgaaatcg
      121 accggggcct tcagcacgga aagccgggac atgttttcac ggatgtcctt caccgtcacc
      181 cgatcataaa aaccggaata ccaggaacac cagatccttg ccggatttac cctggcctgc
      241 tgcctgtccg cgacggcgcg cgcgtacttc tgcagaagca ggttgaaatc gtcttcgaaa
      301 cagatgacaa gcggatccag ttcgcgcgtc tgccccggtt ccagcgtggc gccgtccagg
      361 aaggcgatga tctcgacaag ccgacgcccc aggtcgatag cgacccggcc gaagttaagg
      421 gaaacgccga agaaaccgac cgcgaagcca cgacgcgcct gggagtcgta cagggcagtg
      481 aaccactcgg actcgaactg accgaccttc cttgagaagg tggttccgtc gaaatagacc
      541 atgttccgaa cggccgccgg cagccatgaa tacggcaggc ccttcaggaa gaaggaactg
      601 tccggaaccg tgatcggcgc cgcgcccgac ggcgaccctg tattgaagcc catcttgaat
      661 cgcgaccact ggtcaagatc ccccgaaagg ccgaattcgc cggcgaaccc ggcggttgaa
      721 aagaccgctc gatcaagcgc cagcggaccg tcggacagat tccggatggt ggaaaagacc
      781 ttgaccgtgc cgtcggcttc gcgttccagc cttacagtcg tcgcgacgcg ggacgaatcg
      841 gtcggcttgc caccgaccgg catgacgctc agggaaagat cccccaggct ggcctgaagg
      901 cggccggacg gatcccggat aactccggat tccgggtcac cttcggcatc cacaaaacgg
      961 aaaacctggt cgtgcgtcat cgcaaacccc cgcccgcgga cagccgcgat cagaagcggt
     1021 actggaaatc aaacgcgaat tcgttgttgg ttccgtgacc cttcgcgttt gccggcggcg
     1081 tgccgatcga gtcctggaca tcgatcaggt taaaccagtt gtggacgtac gccaggctca
     1141 cgcgccagtg atcattgatg cgccacctgg ttccgaccga gaaccccttc tggtcaacgc
     1201 ccgggttttc cagggaaagc gtcgatgtcg gattcggggt gtagtccatc tggtatccgg
     1261 ccatcagatc aagttcggga atcggcctgt aaaggatacc aaggcaccag ttccacgaat
     1321 tgccgtagtt cttggcatcg ctgaagccgt tcaggcccgc gacagggctg tcaagcttgc
     1381 ttatctgcat ctggttcgtc tggtagtgcc agtacgacac gtccgcggcg acctcgaaat
     1441 tcgggacgat tgcccagttg atgccggcgc ggatcgacaa cgggatgatc atgttggtgg
     1501 tctgcttcgt cttgccaagc agatcctcgc gcccgtcgat atgaagccgg tacttcgcgg
     1561 tcacgttgcc acgcagatcc agcttggagc cgctggagaa aaccagcccg atgctcaggc
     1621 tcttcaccgg cctcaccaga aggccgatgt cgaatgccca ggtccagttc gaaccctgga
     1681 tgtcaagctg gatgtcgttc ttcatgttcg cttccgtgga attgaagcgg ccatcgtact
     1741 tcgggtcgtt ctggacgtcg gccggggatg gaagcatgcc cgtgttaagg aagcgcgtcg
     1801 cggtcaggta cgtgtaaaga acgctcaggc ttacgccggc gctgatgtag ttgttgaagt
     1861 cgtacgcggc cgacgcggtc aatcttgttg aaaggaaaag cgcctgggtg acgtgatagc
     1921 gggtcggctc gtcaatcggc aggctggcgc catatgcgtt cggggcgtaa atggcaagcg
     1981 cagcgcggaa acgcttcgtc ccgaagtcgg tggcgacgcc gataaacgga atcgcgccga
     2041 tgctgatgtc gggagtgatt tcatggtcgg ccggcagcag gtcgcccttg ctgttgtaca
     2101 gcttcattcc caggtcgagc acgaaggtcg agagggcgaa atagatctgg gttccgttct
     2161 gcagggtcag accggccgga ttgtggaaaa cggccgtcac gtcgtcaggc ttgccgacca
     2221 cggcgaacat tcccattctt cggccgccga agtccagatt cgaatatccg ccggccagcg
     2281 ccacggaagg cagaaggacg ggggcggcga ccagaacggc catcacaagg ctcacgacca
     2341 gtttcgaatt acttttcatc tgtctggctc cctgccggat tgcccgaaat cacgaaataa
     2401 tcagaacgga tgatatgcat ccaggtaaat accaaccggc gaaccggcat ccctggcttc
     2461 cttcgcccat gccaggtctg cccggataat cacggtttca ccgaactgga ttcgcagccc
     2521 gccgccggcc gcgaccttca gcccaagcga gtttccgtca agaagcgcgt ttccgctgta
     2581 atcggcccag acgcgaccga tgttgcagaa gaacgccgtt ccgaacatgc cgcgatactt
     2641 cttgatgcga aacggaaata tctgcgatcg cagctcaagg ttggcgccaa ccttgatttt
     2701 tccatgatag cgcccctcgg gaatcccgga cagccccatt cttccaccga tgaactcgat
     2761 cggttcgaac gcgccggcac gcgccatttc gtaaaaaggc acgttgccga acagcatgtc
     2821 cagcatcagc ctggtcgcga atgaaagctg cggtcccagg atcggaacga aaaagcgggc
     2881 gtcagtgcca aaggcgccat aatgaaggtt gttgtccatg ccgggcgaga atcgcaacga
     2941 actttcgaca tagatgccct tgaccgggtt gtattcatga tccctggtgt cgtagatgac
     3001 gccggccgcg aactgaaggc cgaagtgatc ctgcagcccg taaagtccgt ccatatcggc
     3061 tgccagcttc gaatcctcga acggggtgat ccgcatc
//
LOCUS       DAOPPA010000397         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34293_length_3096_cov_3.595857, whole genome shotgun sequence.
ACCESSION   DAOPPA010000397 DAOPPA010000000
VERSION     DAOPPA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34293_length_3096_cov_3.595857"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKH54_11150"
     CDS             complement(<1..487)
                     /locus_tag="PKH54_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00493.1"
                     /translation="MRSLLSIRIFAVVVATGLFALSCSDGPVLQYDIPGSDNGSDVAG
                     DTIPPKDILPDNQEDRIGEDIGIDLIGVDTNVPDSNGDTGPLPREFGYPCLDNTDCNS
                     GYCVEDVNGLVCSRRCNNDCPDDWSCRTVIVVGDPVTLCMPSGVFLCRSCVKNSQCAD
                     GL"
     gene            complement(684..1610)
                     /locus_tag="PKH54_11155"
     CDS             complement(684..1610)
                     /locus_tag="PKH54_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00494.1"
                     /translation="MSSKSKMIAGGTRLLERRFSRPLFMMAHHDDEISQAGVFQRLGP
                     NIRAVWVTNSDGLYFESDMQPADYGRLRMAEGIASVAQAGIPESSTRCHGWSEVEIYR
                     WLSRLNSGAASMEQAAGFFDCIRDDIRNDIFEIGPDAVFTLAWQGGQPEHDLVHFFTA
                     LAVREYSAKTGSAVEFFHMPAYEYTILVAHRFHPFYRGQRLRISLSEPEMETKLRMIE
                     QYPSQKRLFSDFQKVFSIVGKLGHLTGGPRNATEWLSTEEFGPVPEGLDYTKNTHVFD
                     RCSYINDDFEGAPVTFRGSVRPIVQWYLGKSF"
     gene            complement(1654..>3096)
                     /locus_tag="PKH54_11160"
     CDS             complement(1654..>3096)
                     /locus_tag="PKH54_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00495.1"
                     /translation="GTCGSCQDGKTCFLGTCCTPNCAGLECGDDGCGGQCGQCGDGLP
                     CMAGTCCVPVCGSAKCGEDSCGRPEGCGKCDAGFRCADGQCCKQQCAGMECGDDGCGG
                     TCGECAADRFCESGTCRPNGCEGRWCGTGTGGVDCGSCKTAQGEICFAGSCCTPDCGD
                     DLSEKICGTDGCGGSCGSCEGDLVCCFDGSTCCPKECQGRECGPDGQGGYCGPECPVN
                     HVCTEDGKCCQPYCPGQCGDDGCGGSCGECKAGVDCHNYFCPQTTNECLNNFCESCNP
                     EIEDCEGVASENCRNCPGDCGCIGEYECINDRCCIPECGERECGDNGCGVPCGLCGHG
                     AVCNQSGMCEPCDDECTPGQYCTPDGTAVWTCTYAVEGCNKKLPATPCQFGCSPFIND
                     CMNECPATNGKCSAPGSYKCFNDLNGFPQCVFICPTDPSIPSSERRWTALVNCSASGD
                     SCKCGEFEDIAMCMTSQGGAKPCYGERLDD"
BASE COUNT          677 a         1002 c          840 g          577 t
ORIGIN      
        1 aaagaccgtc cgcgcactgt gaattcttga cgcacgaccg gcagaggaat acccccgaag
       61 gcatgcacag cgtgacagga tccccaacga cgataaccgt ccggcaagac cagtcgtctg
      121 ggcagtcgtt gttgcaacgc ctggaacaaa ccaggccgtt aacgtcctcg acgcagtaac
      181 cggaattgca gtcggtgttg tccaggcacg ggtagccgaa ctcgcgcggc agcggcccgg
      241 tatcaccgtt tgaatcgggc acgttggtat cgactccgat caggtcgatt ccgatatcct
      301 cgccgatgcg gtcttcctga ttgtccggaa ggatgtcctt cggcgggatt gtgtcgccgg
      361 caacgtccga accgttgtcc gaacccggga tgtcatattg gagtaccggc ccgtcggaac
      421 aggacagcgc gaaaagaccg gtcgcaacaa cgacggcaaa aatgcgaatc gaaagaagac
      481 ttctcatgat aaatcctccg ggaacggcga cggactgaaa aacaatgcct aacccgctgt
      541 tttcaagggc caaaaggccc tgccagggaa cacttgagtt aactgcacga ttctatcggt
      601 gaacaggccc tgtgtaaagg aacgctcatg aaaaacacat gaaatatcga gaaaaattca
      661 agggctggaa accgcacgca atgtcagaag gacttaccca gataccactg cacgatgggg
      721 cgaaccgatc cgcggaacgt cacgggggca ccttcaaaat catcgttgat ataggagcat
      781 cgatcaaaaa cgtgcgtatt ctttgtataa tcaagacctt ccggcaccgg cccaaactcc
      841 tcggtggaaa gccattccgt cgcattcctg ggtccaccgg tcaagtgtcc cagcttgccg
      901 acaatcgaga aaaccttctg gaagtctgaa aagagcctct tctgggaagg atactgttca
      961 atcatcctca acttggtttc catctccggc tccgagaggc ttatccgaag cctctgcccc
     1021 ctgtaaaagg gatggaacct gtgggccaca aggatcgtgt actcgtaagc cggcatgtgg
     1081 aagaattcga cggccgatcc ggtcttcgcg gaatactcgc gaacggccag tgccgtgaag
     1141 aaatgaacca ggtcgtgctc gggctggccc ccctgccacg ccagggtgaa aaccgcgtcg
     1201 gggccgatct caaatatgtc gttcctgata tcgtccctga tgcagtcaaa aaagccggca
     1261 gcctgctcca tcgaggcggc gcccgaattc aggcggctga gccagcggta gatctcgacc
     1321 tccgaccacc cgtgacaccg ggtggaggac tccgggattc cagcctgggc caccgatgcg
     1381 atgccttcgg ccatcctcag cctgccatag tcggccggct gcatgtcgga ttcaaaatac
     1441 aggccgtcgc tgttggtaac ccagacagcc cggatattgg ggcccaggcg ctgaaagacc
     1501 ccagcctggc tgatctcgtc gtcgtgatgg gccatcatga acagcgggcg cgaaaaacgc
     1561 ctttcaagca accgcgtgcc gcctgcaatc atttttgact tactggacaa tgtgtcgcct
     1621 ccggataatc gtaaatggga atccagaccg gagtcagtcg tcaagccttt cgccgtaaca
     1681 cggcttcgcg ccgccctgac tggtcatgca catcgcaata tcctcgaatt cgccgcactt
     1741 gcaggagtcg ccggacgccg agcaattcac cagcgcggtc caccgccttt cgctggaagg
     1801 aatcgacgga tcggtggggc agatgaacac gcactgcggg aacccgttca agtcattgaa
     1861 acacttgtag ctcccaggtg ccgaacactt accgtttgtc gcggggcatt cgttcatgca
     1921 gtcattgatg aacggggaac agccgaactg gcacggcgtc gccggcaact ttttgttgca
     1981 gccctcgacc gcgtaggtac aggtccatac ggcggtgccg tcgggcgtgc agtactgtcc
     2041 cggcgtgcat tcgtcatcgc acggctcgca catcccggat tgattgcaga cggccccgtg
     2101 accacaaagc ccgcatggaa ccccgcaccc gttgtccccg cattcacgct cgccgcactc
     2161 ggggatgcag cacctgtcgt tgatgcactc atactcgccg atgcacccac agtccccggg
     2221 acagttgcgg cagttctccg acgccacgcc ttcgcagtcc tcgatctcgg gattgcagga
     2281 ctcgcagaag ttgttcaggc attcattggt cgtctgcgga cagaagtagt tgtggcagtc
     2341 aacgcccgcc ttgcactcgc cgcagcttcc gccgcagccg tcgtcgccgc actgcccggg
     2401 acagtaaggc tgacagcact ttccatcttc ggtgcagacg tggttgaccg ggcactccgg
     2461 cccgcaatag ccgccctgcc cgtcaggccc gcattcacgt ccctggcatt ccttcggaca
     2521 gcacgtgctg ccgtcgaaac agcacaccag atcaccttca caggaaccgc atgagccacc
     2581 gcaaccgtcg gtgccgcaaa tcttttccga aaggtcgtca ccgcagtccg gggtgcagca
     2641 cgaaccggca aagcatatct cgccctgggc ggtcttgcac gatccgcaat caaccccgcc
     2701 cgtccctgtt ccgcaccagc gtccttcgca accattcggg cggcaagtgc cggactcgca
     2761 gaatctgtcg gcggcgcact cgccgcaagt accgccgcaa ccgtcgtcgc cacattccat
     2821 cccggcgcac tgctgcttgc agcactggcc atcggcacac ctgaaaccgg catcgcactt
     2881 gccacaacct tccggccgcc cgcaactgtc ttcaccgcac ttcgccgaac cgcaaaccgg
     2941 aacacaacat gtgcccgcca tgcagggcag cccgtcaccg cactgcccgc actgtccgcc
     3001 gcaaccgtca tccccgcact caagcccggc gcagttgggg gtgcagcacg tgccaaggaa
     3061 acatgtcttg ccatcctggc aacttccgca tgttcc
//
LOCUS       DAOPPA010000398         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34356_length_3092_cov_3.054659, whole genome shotgun sequence.
ACCESSION   DAOPPA010000398 DAOPPA010000000
VERSION     DAOPPA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34356_length_3092_cov_3.054659"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3092)
                     /locus_tag="PKH54_11165"
     CDS             complement(<1..>3092)
                     /locus_tag="PKH54_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD00496.1"
                     /translation="VSEAMRIRLAYLFDPRLAVHTSRVTPYPHQITAVYECMLPRQPL
                     RFLLADDPGAGKTIMAGLLIKELMVRGDVLRCLIVCPGNLGEQWQDELNEKFDLRFQI
                     LTNDNLEASRTGNWFVENNLVIARLDKLSRNENIQKMLQAPDAMWDLVVCDEAHKMAA
                     TVFGGEIKYTKRYHLGELLAGVTRHFLLMSATPHNGKELDFQLFMALLDRDRFETSPK
                     TSAHASDVSDVMRRMVKEKLLKFDGTRLFPERIAYTVPFKLSPGEADLYQAVTAYVRE
                     EFNRVERLGSDKRKATVGFALTILQRRLASSPEAIYQSLRRRRERLEDRLVEAETRQA
                     QVFDTVLSESILPPIDMESLDDFEDAPDAELAAAEDEIVLKATAASTIAELKAEIQTL
                     SGLESAAAVVTRSDHDTKWAELAGVLDMVFRPVTEELGRAAEALQQGQDVLDFTPSPH
                     QKIVIFTEHRDTLNYLHRKISTLLGRREAVIDIHGAMNRKDRRAAEQAFINDPQVQVL
                     IATDAAGEGINLQRAHLMVNYDLPWNPNRIEQRFGRIHRIGQSEVCHLWNLVADETRE
                     GDVYRRLLEKLEQARQALGGQVFDVLGKLQFGDESLRDLLIRAIRYGDQPDVRARLTQ
                     VVESALDREHITALLDENALAHEVMDSTRVQLIREDMERADIRKLQPFYVGSFFVNAF
                     RQLGGEMREREPRRYEITNVPRSIREQVEKMAFGKSIPSRFERVTFDKDQQNPPDKPR
                     AEFLCPGHPLMDAVLELTMSNNRDVLRAGAALVDETDSGTQPRIVYYLEHALRDAVQT
                     RAGEFRDISRRMMFIEVESTGTIRNIQYAPYLDYRPLNSDEPAPSQILERPECAWIQG
                     SGESKIMDHAVSTVVPKHLEEVRKYRLDLIARTRAAVQERLSSEIRRWDKRAAELKAQ
                     ERAGKVNAKLNWAQAQARAEELAQRQRARLDQLKLEEEIRPAVPVVVGGVLVVPIGLI
                     RLMSGETSPTSQHPVDTQAAAARAREIVMQAEQSLGFEPVDREFERLGYDIESRDPKG
                     G"
BASE COUNT          610 a          875 c          921 g          686 t
ORIGIN      
        1 cccccttttg ggtcgcggct ttcgatgtca tagcccagac gttcgaattc cctgtcaaca
       61 ggttcgaagc ccagggactg ctctgcctgc ataacgattt cccgcgccct ggccgcagcg
      121 gcctgtgtat caacgggatg ctgtgacgtc ggcgatgttt cgcccgacat cagacgaatc
      181 aggccaatcg gcacgaccag aacgccgccg acaacaaccg gtaccgctgg cctgatttct
      241 tcttccagtt tcagttggtc cagacgggcg cgctggcgtt gtgccagttc ttcggcacgg
      301 gcctgagcct gcgcccagtt gagtttggca ttgaccttgc cggcccgctc ctgtgccttc
      361 aattccgcgg cgcgcttatc ccagcgccgg atttcggacg atagccgttc ctggacggcc
      421 gcgcgggtcc tggcgatcag gtcaaggcga tacttcctga cttcttcaag atgtttcggc
      481 accacagtgc tgacggcgtg gtccatgatt ttcgattcac ccgatccctg aatccaggcg
      541 cattcggggc gttcaagaat ttgggacggg gcgggttcat cgctattcaa gggccggtaa
      601 tccagatacg gtgcgtactg gatgttgcgg atggtccccg ttgattcgac ttcgatgaac
      661 atcatccgcc gggaaatatc gcgaaattcg cctgcacggg tctggacggc gtcccgaagg
      721 gcgtgttcca gatagtaaac aatgcgcggc tgtgtgcccg aatcggtttc atccaccagc
      781 gcggcgccgg cgcgaaggac gtcgcgattg ttggacattg tcagttccag cactgcgtcc
      841 atcagtggat gaccggggca cagaaattcc gcgcgtggct tgtcgggcgg gttctgttga
      901 tccttgtcga aggtaacccg ttcaaaccgg gacggaatgg atttcccgaa ggccatcttt
      961 tcgacctgtt ccctgattga acgtggcaca ttcgtgattt cataacgccg gggctcgcgt
     1021 tcccgcattt caccgccaag ctggcggaac gcgttgacaa aaaatgaacc aacgtaaaac
     1081 ggctgaagct tgcgtatatc ggcacgttcc atgtcttcgc ggatcagttg tacgcgggtt
     1141 gaatccatga cctcgtgcgc aagcgcgttt tcgtccagaa gcgcagtgat atgttcacgg
     1201 tccagcgcag attcgacgac ctgggtcaga cgcgcgcgaa catccggctg atccccgtac
     1261 cgaatggcgc ggatcagcag gtcgcgcagg gattcatcgc cgaattgcag tttgccaagc
     1321 acgtcgaaca cctggccgcc aagcgcctgt cgggcctgtt ccagtttttc cagcaggcga
     1381 cgatagacgt cgccttcgcg tgtttcgtcg gcaaccagat tccacaggtg gcaaacctcc
     1441 gactgcccga tacggtgaat tcgaccgaaa cgctgttcaa tccggtttgg attccacggc
     1501 aggtcgtaat tcaccatcag gtgggcgcgc tgcaggttga tgccctcgcc ggcggcgtcg
     1561 gtggcgatca gtacctgaac ctggggatcg ttgatgaatg cttgttcggc ggcgcgccgg
     1621 tccttgcggt tcattgcgcc atgaatgtcg atgaccgctt cgcgacggcc aagcagggtt
     1681 gatatcttgc gatgcaaata gttgagcgtg tcgcggtgtt cggtgaagat gacgattttc
     1741 tgatggggcg acggcgtgaa gtccaggaca tcttggccct gctgaagtgc ttcggcggcg
     1801 cggcccaatt cttccgtgac ggggcgaaac accatgtcca gaacaccggc aagttcagcc
     1861 catttcgtgt cgtggtcact ccgtgtaaca acggcggcgg ccgattcaag accggacagc
     1921 gtttgaattt ccgccttcag ttcggcgatc gtgctggctg cggtggcctt cagcacaatt
     1981 tcatcttcgg ctgccgcaag ttccgcatcg ggcgcatctt cgaaatcatc cagactttcc
     2041 atatcgatcg gcggaagaat cgattcggac agcaccgtgt caaaaacctg cgcctgacgg
     2101 gtttcggcct cgacaaggcg gtcttcaagc cgttcgcggc ggcgacgaag cgattgataa
     2161 atcgcttccg gggacgatgc aagacgacgc tgaagaatcg tcagggcgaa gccgacggtc
     2221 gccttgcgct tgtcggaccc caggcgttca acgcgattga attcttcacg gacatacgcg
     2281 gttactgcct gataaagatc ggcttcaccg ggcgacagct tgaacggaac ggtataggcg
     2341 attcgttccg gaaaaagcct ggttccgtcg aacttcagca gtttttcctt gaccatacgg
     2401 cgcatcacat cggaaacgtc cgatgcgtgg gccgaggtct tgggactggt ttcaaagcgg
     2461 tcccgatcaa gaagcgccat gaaaagctgg aaatcgagtt ccttgccgtt gtggggcgtg
     2521 gccgacatca acaggaagtg gcgggtcacg ccggcaagca gttcgcccag gtgatagcgt
     2581 tttgtgtatt tgatttcgcc gccgaaaacg gtcgcggcca tcttgtgcgc ttcgtcgcat
     2641 acaaccagat cccacatggc atcgggggcc tgcaacatct tctggatgtt ttcgttgcgg
     2701 gaaagcttgt ccaggcgggc gatgacgaga ttgttttcga cgaaccagtt gccggtgcgg
     2761 gacgcttcca gattgtcgtt cgtcagtatc tggaaacgca ggtcgaattt ttcgttaagc
     2821 tcatcctgcc actgttcacc gaggttaccc gggcaaacga tcaggcagcg cagaacgtcc
     2881 ccgcgtacca tcaattcttt gatcagcaaa cccgccatga tggtcttgcc ggcgccaggg
     2941 tcatcggcca gcaagaaacg gagcggctga cgcgggagca tgcattcgta aacggcggta
     3001 atctggtgcg gatacggcgt gacacgcgac gtgtggacgg ccagtcgagg atcgaaaaga
     3061 tacgccaggc gaattcgcat cgcttcggac ac
//
LOCUS       DAOPPA010000399         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34472_length_3083_cov_4.608983, whole genome shotgun sequence.
ACCESSION   DAOPPA010000399 DAOPPA010000000
VERSION     DAOPPA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34472_length_3083_cov_4.608983"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1113
                     /locus_tag="PKH54_11170"
     CDS             193..1113
                     /locus_tag="PKH54_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00497.1"
                     /translation="MRNFVCVRMIMARSVLVLLPVLSAVACSSGPDPATGECMERETA
                     LNGKLAECQASATSATSACAASQKAASDELAAVKARLETLERENAELKQTPAAVSQEI
                     LTAVDSADTIAKIDDVLASISAFGDRFPGAPEIRVVTRKVSGLKSARAKLVRAEEQAK
                     ALQAISDIKALLAGAEDADELSFTQILAVANHLSSNNIKYSTIAQLPRGNFGEAMKDP
                     DSERGKSMVISGRVVQISKDGDYFKGLICTGDFCDRVYYFITPGTTRGISQESYVSFA
                     GVLAQRYSYSNSGGGTTHSVALVGYFKGQE"
     gene            1336..2472
                     /locus_tag="PKH54_11175"
     CDS             1336..2472
                     /locus_tag="PKH54_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD00498.1"
                     /translation="MMFNARFAYTDSMVGNLMRIEAARRVVDILPLPPAGILDLKRVA
                     RQKSSRGSVAIEGNPLDEAGALRAIAAADRSPDEHQQEIRNYWRALEWLEDRVAAGLV
                     IDQGFIKALHGIIIVRTGGGRRGPSEYRSTECPVVDVVTRTIDYAPPRPRDVPALMAD
                     LAAWVESDRARALPGPVAAALLAHRFVSIHPFNDGNGRTARALATAALWRSGYYMQGF
                     LSVEELYNSKRGEYYDSLQMGLPADFYSGRHDADHTRWIEYFVRVLYDASTQIGQQAE
                     RLYGAHAVDVPWSTLSRRQQQFLVRLSTRSLTHGAPAPSFTPSDVMDWFDVSRPTAYD
                     WLDDWTNQRFIEPADVGLRIRRYRLTPSWAELLESVRLTAVARS"
BASE COUNT          660 a          873 c          874 g          676 t
ORIGIN      
        1 atgccagcag ctcgcgtcgc atcccacgcg ttcgtgccga ttcccgactt tgacagccgc
       61 tggaaccccg accatactcg tctcaaggag gcgttttgtg gatgaaagtt cctacacgca
      121 tcataagcag gaccctggtg tgacgggata ttgtttcccc gaactgacgg gatccacatc
      181 aaggaggttc agatgcggaa ttttgtttgt gttcggatga ttatggcgcg ctcggtcctg
      241 gttcttctgc ccgtcttgtc tgccgtcgcc tgctcatcag gtcccgaccc ggcgactggc
      301 gaatgtatgg aacgcgaaac ggcgttgaac ggcaagctgg ccgaatgtca ggcgtctgca
      361 acatccgcaa cgtctgcctg cgcggcatcg cagaaggctg cctccgacga actggccgca
      421 gtgaaagcga ggcttgaaac cttggaacgg gaaaacgccg aactgaagca gacgccagcc
      481 gcggtttcgc aggaaatcct gaccgcggtg gactcggccg atacgatcgc aaagattgac
      541 gacgtcctgg cttcgatatc ggccttcggt gaccgcttcc cgggcgcgcc ggaaatccga
      601 gtcgttaccc gcaaagtatc gggactgaaa tctgcccgcg ccaagctggt cagagccgaa
      661 gaacaggcca aggcgcttca ggcaatcagt gacatcaagg cattgcttgc aggcgcagaa
      721 gacgctgacg aactttcctt tacccagatc ctggctgtcg ccaatcatct gtccagtaac
      781 aacatcaagt actcgacaat cgcccagctt ccccggggaa atttcggcga agccatgaag
      841 gaccctgatt cggagcgcgg caagtccatg gtgatcagtg ggcgggtagt tcagatttcc
      901 aaagacggcg attacttcaa agggcttatc tgcacaggtg acttctgcga ccgggtctat
      961 tacttcatca cgcccggaac cacccgcggc atcagtcagg aaagctacgt ttcattcgcc
     1021 ggcgtccttg cgcaacggta ttcgtattca aattccggtg gcggcaccac tcactccgtg
     1081 gccctggtcg gttacttcaa gggacaggaa tagcctcaga tcaactcccg cctgcaaaag
     1141 aaaaacttga aataggtaaa gtcggcggtt cgaaataaga taagttttcc gcgacaaacc
     1201 gagtggtgct gaactgtttt cacttgttga aatcacaatg atttcagtgt agatatgcga
     1261 tatgaccgat ctcgcgattg atgaaaataa gacaagtcgt caactactga ataagattag
     1321 ttggggagac cgacaatgat gttcaacgcc aggtttgctt acacggattc gatggtcggg
     1381 aatttgatgc gaatcgaagc ggccaggcgc gtggtggaca tcctgcctct gccgcccgct
     1441 ggaatccttg atctgaagcg tgttgcccgg cagaaatcgt cgcgtggctc ggtcgcgatc
     1501 gagggaaatc cgctggacga agccggtgcc cttcgcgcca tcgccgccgc ggatcgatcg
     1561 ccagacgaac atcagcagga aatccgcaat tactggcggg cgcttgaatg gctggaagac
     1621 cgggttgctg cgggactggt aatcgaccag ggcttcataa aggcgttgca tggcatcatc
     1681 atcgtgcgga ctggtggcgg gcgccggggt cccagcgagt accgcagcac ggaatgcccg
     1741 gttgttgacg tggtcacgcg tacgatcgat tacgcaccgc cgcggcctcg cgatgttccg
     1801 gcgttgatgg ccgatcttgc cgcatgggtc gaatccgaca gggcccgggc gcttcccgga
     1861 cccgtcgctg ctgcactgct ggcgcatcgt ttcgtaagca ttcatccttt taacgatgga
     1921 aacgggcgta ccgcgcgtgc gcttgccacg gcggcgctgt ggcgttccgg ctattacatg
     1981 cagggcttcc tgtcggtcga agagttgtac aacagcaagc gtggtgaata ctacgacagc
     2041 cttcagatgg gcctgcccgc ggatttctat tcgggacgcc acgacgccga tcacacccgg
     2101 tggatcgaat acttcgtcag ggtcctgtac gacgcgtcca cacagatcgg acagcaggcc
     2161 gaacgccttt atggcgctca cgctgtggat gtgccctggt caaccctttc acggcgtcaa
     2221 cagcagttcc tggtgcgttt gtccacgcgc agtctgacac acggcgcgcc ggccccttcg
     2281 ttcaccccgt cggatgtcat ggactggttt gacgtttccc ggcccactgc ttacgactgg
     2341 ctggatgact ggacgaatca gcgctttatc gaacccgcgg acgttgggct ccgcattcgc
     2401 cggtatcgcc tgacgccatc ctgggccgaa ctgctcgaat cagtcagact gaccgcggtt
     2461 gcgcggtcct agcgaaactg ccgcctcgac cgtcaaagtc gtttccggca gtacgaagca
     2521 gccccgtgtc cgccgtgatc caatcaaatg acgtcccgca catccataac tgctgcgcct
     2581 ttcttatgga atcaatcagc agagaaatga gagcctgaat gttgccctga cctcgttcaa
     2641 cacggcggtt caattctcta catggcgtgt agagaattca tcttatgccg ttgagttcat
     2701 cctgaaggtc gcggatggtc gccatgattt cgctcaacga tggaacccgg ccgaaaatca
     2761 tgtggcgcat ttcggcgtat ctaacagact ggcggcggca gtccaatggt aacattccga
     2821 ctgtaaatgg ttatcctggt gaagctttgc gtttcaaatt tgctgtaatc gatactggcc
     2881 gatgccaccg ccgggtgtag caccacggat catttccgtg atgccacgaa catgaccgaa
     2941 atgcgcaggg aagggcgcgg cgtcgcggtt gtcagttgat tgtcactgaa ttgcaaaagt
     3001 taccgttttg cgctaacttc tgcatttgaa tgcgtattcc ggaccaatcc gatcaccgat
     3061 tccagtgatg tcgatcaccg att
//
LOCUS       DAOPPA010000400         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34522_length_3080_cov_3.777521, whole genome shotgun sequence.
ACCESSION   DAOPPA010000400 DAOPPA010000000
VERSION     DAOPPA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34522_length_3080_cov_3.777521"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKH54_11180"
     CDS             <1..1016
                     /locus_tag="PKH54_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00499.1"
                     /translation="CFKQLGSATGFCVEECSETECTDTARFECSDNGKGVDICQPTTE
                     GCGGCSEPTPFPFEGGCVDCRNTDDCGANEMCNTLEHKCVPSTCGDDTIMCEADMKCH
                     QCCTDDDCLRFPEATGVCLEDGTCQGVEPCGGLCTDDFPICALVQGVEQCVQCAIDTD
                     CQAINSVCTCVGDPLYSCIDDVGAICQSPGVFCQATCEDAGDCPPTSTGGAMECAMIN
                     GGTTGFCYDPVGDCDGSVYCCAPGKKCFDLILLMNSGMMGSTGGTPMDPSMLQSGSGY
                     CECSVAEDCINGKSCTDMSVLCVVANLVVGYEDLVCPGGVLSPTMPPKLCVDFADLLA
                     GIF"
     gene            1184..1531
                     /locus_tag="PKH54_11185"
     CDS             1184..1531
                     /locus_tag="PKH54_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD00500.1"
                     /translation="MSRDFEAVIGKDIVIKGEIEGTQDLLVEGRVEGTVRLQSELVVG
                     PAGLVDAEVEGPAMTVEGQFNGTANCGDLITLAAGCHGTGELKAPRIVIEEGAAFTGT
                     LNMETGIQDGRGE"
     gene            1535..1858
                     /locus_tag="PKH54_11190"
     CDS             1535..1858
                     /locus_tag="PKH54_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD00501.1"
                     /translation="MADTIIGSKTRVNGVISGRDRLVVAGLVEGQVAMEADVLVEAPG
                     RVEASVRAQGVFVHGTVNGEVAGAAKVEIATDGRMIGDVRTGRILISDGAVFKGRVEM
                     DVTEG"
     gene            1863..2444
                     /locus_tag="PKH54_11195"
     CDS             1863..2444
                     /locus_tag="PKH54_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD00502.1"
                     /translation="MADRITEIGPASLVKGEIEAQEDVSVFGRVEGIVKSTETVTIEE
                     EGLVDGEIEAANVVVAGVMIGNIRATARVDVLETGVVQGDIATPVMVLLDGGAIKGRL
                     VMDDSAPEPVRVNTTGTRSRPAAGRVTGQPTVSRTAPRPAAQTKSTGATGRATVAART
                     DVKSRPTASRRYAEEPAPVEPSRRDTDLLDGND"
     gene            2495..>3080
                     /gene="pyrE"
                     /locus_tag="PKH54_11200"
     CDS             2495..>3080
                     /gene="pyrE"
                     /locus_tag="PKH54_11200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOD00503.1"
                     /translation="MDRASFERDRARLLELVARIAYERRQVVLRSGRVSDYYMDCRRV
                     TLHPEGSTLCGRVFWQTYLDSGLRVAAVGGPTLGADPLVTAFQITAFSEGVELPAFLV
                     RKQAKEHGTGSRVEGRFGLPAGADVLLLEDVLTTGISLTEAREGVVADGYNPVACMVL
                     VDRDEGGRQTLEATGLKVIPVFSASEVAAKYDRLN"
BASE COUNT          583 a          907 c         1013 g          577 t
ORIGIN      
        1 gctgcttcaa gcagttggga agcgcgaccg gtttctgcgt cgaggaatgc agcgagaccg
       61 aatgcaccga cacggcccgt tttgagtgca gcgacaacgg caagggcgtc gatatctgcc
      121 agcccacgac cgaaggctgc ggcggatgca gcgagccgac cccgttccca ttcgagggcg
      181 gctgcgtcga ttgccggaac accgacgact gcggtgccaa cgagatgtgt aatacgcttg
      241 aacacaagtg cgtccccagc acctgcggcg acgacaccat catgtgtgaa gcggacatga
      301 agtgccatca gtgctgcacc gacgacgact gcctgcgctt cccggaagca accggcgtct
      361 gccttgaaga cggcacgtgc cagggtgttg agccgtgcgg cggcctgtgc accgacgact
      421 tcccgatctg cgcccttgtc cagggcgttg aacagtgcgt tcagtgcgcc atcgataccg
      481 actgtcaggc gataaattcc gtctgtacct gcgtcggcga cccgctttac agttgcatcg
      541 atgatgtggg cgcgatttgc cagagccccg gcgtattttg ccaggcaacc tgcgaagacg
      601 ccggcgactg ccccccgaca tcgaccggcg gcgccatgga atgcgcgatg attaacggcg
      661 gcaccacagg tttctgctat gacccggtcg gcgactgcga cggctcggtt tactgttgcg
      721 ccccggggaa gaagtgcttc gaccttatcc ttcttatgaa ctccggcatg atgggctcca
      781 cgggcggcac gccgatggat ccgtccatgc tgcagagcgg ttccggatac tgcgagtgca
      841 gcgtggccga agactgcatc aacggaaagt cctgcaccga catgtcagtg ttgtgcgttg
      901 tcgctaatct ggtcgtcggc tacgaagacc tggtctgccc cggtggagtg ctttctccaa
      961 ccatgccgcc gaagctgtgt gtagatttcg cggatcttct ggccggaata ttttgatccg
     1021 ttggcttgcc agcccgccca ttcttcggtt gttgaatccg gcgccgcaag tcgaaatggc
     1081 ttccacgaac agggaactcc cgctcaaggt gcgccttgac tggaacccgt tactgggcta
     1141 agtttcaccg tcaatgtttt ggtctgacaa ccccggggta aacatgagca gggattttga
     1201 agccgtcatc ggcaaagata tcgtaatcaa aggcgaaatc gagggcaccc aggatctgct
     1261 ggtcgagggt cgcgtggaag gaacggtgcg tttgcagtcc gaactggtcg ttggtccggc
     1321 tggcctggtc gatgccgagg tcgaaggccc ggcgatgacg gtcgagggac agttcaacgg
     1381 cacggcgaac tgcggcgacc tgatcacgct tgcggccggt tgtcacggca ccggcgaatt
     1441 gaaggccccg cgcatcgtca tcgaggaagg cgcggcgttt accggcacgt tgaacatgga
     1501 aaccgggatc caggacgggc ggggggaatg atccatggct gacaccataa tcggttcaaa
     1561 aaccagggtt aacggcgtta tcagcggcag ggatcgtctg gtcgtcgccg gcctggtcga
     1621 aggtcaggtc gcgatggagg ccgatgtcct tgtcgaagcc ccgggtcgcg tcgaggcctc
     1681 ggtcagggca cagggcgttt tcgtgcacgg cacggtcaac ggcgaggtcg ccggtgccgc
     1741 caaggttgag attgccacgg acggccgcat gatcggcgac gtccgcaccg gcaggatcct
     1801 gatttccgac ggggccgttt tcaagggccg agtcgagatg gacgtaacgg agggctgacc
     1861 agatggccga cagaatcact gaaatcggac cggcgtccct tgtgaagggc gaaatcgagg
     1921 cccaggaaga cgtgtcggtg tttggtcgcg tcgaagggat tgtcaaatcc accgaaaccg
     1981 tgacgattga agaagaaggg cttgtcgatg gcgagatcga ggccgcgaac gtggtcgttg
     2041 ccggggtgat gattggaaat atccgggcaa cggcacgcgt cgatgtgctg gagaccggcg
     2101 tcgtgcaggg cgacatcgcc acgccggtga tggtgctgct cgacggcggg gccatcaagg
     2161 ggcgcctggt catggatgat tccgctcctg aaccggtcag ggtcaatacc accggaacca
     2221 ggtcgcgtcc cgcggccggc cgggtcaccg gacagccgac agtttcccga accgcaccgc
     2281 gcccggcggc ccagacaaag tcgaccggcg ccaccggccg cgccacggtc gccgcaagaa
     2341 ctgacgtcaa gtcccggccg acggcatcca ggcgttacgc cgaggaaccg gccccggtgg
     2401 aacccagccg cagggatacc gatctgctgg acggcaacga ctaatccaca ccgtttgttt
     2461 gcggccgcct tccggccgcg tatcggggga cagcatggac agggcatcgt ttgaaaggga
     2521 tcgtgccagg ctgcttgaac tggttgccag gattgcgtac gagcggcgcc aggtcgtcct
     2581 gcgatccggc cgtgtttccg actactacat ggattgcagg cgcgtgacgc ttcatcccga
     2641 aggttcgacc ctttgcggcc gcgtgttctg gcagacctac ctggacagcg gccttcgcgt
     2701 ggcagcggtc ggcggtccga cgcttggggc ggatccgctg gtcaccgcat tccagatcac
     2761 cgccttttcc gaaggcgtgg aactgccggc cttcctggtt cgcaagcagg caaaggaaca
     2821 tggcacgggg tcccgcgtcg aggggcgatt cggccttccc gctggcgccg acgtactcct
     2881 tcttgaagac gtccttacaa ccgggatttc cctgaccgag gcgcgcgagg gtgttgtcgc
     2941 cgatggttac aacccggttg catgcatggt cctggtcgat cgcgacgaag gcggccgcca
     3001 gacactggaa gcgacaggcc ttaaggttat tccggtattt tctgcttccg aagttgccgc
     3061 gaagtacgac aggctcaatc
//
LOCUS       DAOPPA010000401         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34618_length_3073_cov_3.856528, whole genome shotgun sequence.
ACCESSION   DAOPPA010000401 DAOPPA010000000
VERSION     DAOPPA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34618_length_3073_cov_3.856528"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1707
                     /locus_tag="PKH54_11205"
     CDS             58..1707
                     /locus_tag="PKH54_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00504.1"
                     /translation="MKTLRPASLSFGVIILATATSFTACRVDDNTPLEQDHNATHRRI
                     IDIRATALQKDSLGKTTKPRPFGIINGSPVVPEGKELGAFYQTSGVRAARYPLGWGCD
                     ITLDYIFDNESADPDSNESYRRVGFDSVTKDLIRYKILPIYQAAWDIGTDGCQPTETI
                     GVSTRPIAAYDNWARVVAEQARYLNSTIKSFYNKPEMIEKLAESGWRPGYVEIYPDAT
                     DAGGYGDLDIGSVASAFKAFQEIISETIPDDGHRLFGVVTPGLRISSPAELTAGYSAI
                     ADFIANLPTNPAHLPDMFTIKPEVDSISSLIAVTDAVRAALDDAGHADVPLAAFDLNV
                     TPETWENLSVFADTIESRSAWLGALTATAYIRLQSKLLTIAPGRWSTLDQAELGPKSG
                     EDLFQYPSSGLQLPAMPMTKAIRLFAEEGTELLAIENEPVDDAPGDVDDSGFKNDLFS
                     VLASRRADGGYQFLVAALNPADQERRGLNMQYQLNITDLAAGTTGWQVAVYETDDTTQ
                     YLPTSPARRGIVKVDEGKVSVAGIIKAPAVHLIQLLPPSSE"
     gene            complement(1820..>3073)
                     /locus_tag="PKH54_11210"
     CDS             complement(1820..>3073)
                     /locus_tag="PKH54_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00505.1"
                     /translation="IGLYGSQPGTTLQGVICQNDVACKGGICTDIGDGKPRCTEECSL
                     ANDLCPSGYHCETMGGQDICVTGSKAGGIGSRCSNSDDCVEGKCDTWPDGVRSCRIMC
                     RSDKECPDGEYCWFKGSYGGCFPEVEVVLKGTGEYCTAGDQCESGKCVFGDDGMRCRV
                     VCTDSDKCGEGFVCDEQSSTCLPGQEPVEPTLEVGDECSLPSLCRTGLCLQGSDGKRC
                     REACGTSANCADGWYCETELSAGACLPGEPPVDLLEEGDVCSSDDECRSGLCFQPTAT
                     DTKRCRVECDLADWFCPTAGTACVGFDTVDSGVCMPVDDRGEVGDSCQVGSECVSGLC
                     LGPKDDFYCSQQCVDDWCPDGFECTQVRDSGKFCIRSAAEVPDGGCSAGSGGGGDLAP
                     LVAMFLIVGLALAGRRFRKSIIDRN"
BASE COUNT          672 a         1006 c          822 g          573 t
ORIGIN      
        1 aaacggcctg cgggatggta agatccacgc ggctggaatg catccggaag gagaacgatg
       61 aaaaccctgc gaccggcatc cctttctttc ggcgtaatta ttcttgcgac ggcaacgtcc
      121 ttcacggcct gccgggtcga cgacaacacg cccctggagc aggatcacaa cgccacacat
      181 cgacggatca tcgacatcag ggcaacggcg ctgcagaagg attccctggg caagaccacc
      241 aagcccaggc cgttcgggat catcaacggc tctcccgtgg tgccggaagg caaggaactc
      301 ggtgcgttct accagacctc cggcgtcagg gctgccaggt atccgcttgg atggggctgc
      361 gacatcacgc ttgattacat attcgacaac gaaagcgcgg atcccgacag caacgaatcc
      421 tacagacggg tcggattcga ttcggtcacc aaggatctta tcaggtacaa gatcctgccc
      481 atttaccagg ccgcatggga tatcgggacg gacgggtgcc agccgactga aaccatcggg
      541 gtttcaaccc gaccgattgc cgcttacgac aactgggccc gtgttgtggc cgaacaggcc
      601 aggtatctta attcaacgat caagtcgttc tacaacaagc ctgaaatgat tgaaaagctg
      661 gcggagtcgg gttggagacc gggatacgtt gaaatatatc cggatgcgac cgacgcgggc
      721 ggttacgggg atctggacat cggctccgtt gcttcggcat tcaaggcatt ccaggaaatc
      781 atttccgaaa cgatacccga cgacgggcac aggctgttcg gcgtcgtgac cccggggctt
      841 cgtatttcat cacctgctga gctgaccgcg ggatattcgg ccattgccga cttcattgca
      901 aaccttccga ccaacccggc ccaccttccc gacatgttca cgattaagcc cgaagttgat
      961 tcgatttcat cgctgatcgc ggttaccgac gcggttcgcg ccgctcttga cgacgccggc
     1021 catgccgacg ttccccttgc cgcgttcgac cttaacgtca caccggaaac ctgggaaaac
     1081 ctttccgttt tcgccgacac gatcgaatcg cgaagcgcct ggcttggcgc tttgaccgca
     1141 accgcataca tccggcttca atccaagctt ctgacgattg cgcccggccg ctggtcaacg
     1201 ctggatcagg ccgaactggg accgaagtca ggggaggatc ttttccagta tccatccagc
     1261 ggccttcagc ttccggccat gccaatgact aaggcaatcc gcctcttcgc cgaggagggg
     1321 acggaactgc ttgccatcga aaatgagccg gtcgatgacg cgccggggga tgtcgatgat
     1381 tccggcttca agaacgatct gttttccgtg cttgcatcgc gaagggccga cggcggctat
     1441 caattccttg tggccgcgct gaacccggcc gaccaggaac gacgcgggtt gaacatgcag
     1501 taccagttga acatcaccga cctggcggct ggcaccaccg gctggcaggt cgccgtctat
     1561 gagacggacg acacgaccca gtacctgcca acgagccccg caagacgtgg aatagtcaag
     1621 gtcgatgaag gaaaggtcag cgttgccggc attatcaagg cgccggccgt gcacctgatt
     1681 cagttgcttc caccgtcatc cgagtaattc cgccgcccga tgcccccggc acgaattcga
     1741 ccagccctta ccgattactg acccaggcgg ggttgaattg ataagcacaa cgggcttctt
     1801 cgaaccgtgt ccgcgacttt caattgcggt cgatgattga cttgcggaac ctgcggccgg
     1861 ccagcgccag ccccacgata aggaacatcg cgacaagcgg ggccaggtcg ccaccgccgc
     1921 cggaaccggc cgaacatcca ccatcgggaa cttcagccgc ggaccttata cagaacttgc
     1981 cggaatcgcg aacctgggtg cactcgaaac catccggaca ccagtcatcg acgcactgct
     2041 ggctgcaata aaaatcatcc ttcggcccaa ggcaaagacc cgaaacacat tcgcttccca
     2101 cctggcacga gtccccgact tcaccacgat catcgaccgg catgcagacg cccgaatcga
     2161 ccgtgtcgaa accgacacac gcagttcccg ccgtcggaca gaaccagtcg gcaagatcgc
     2221 actcgacgcg gcaacgcttc gtatcggtcg ccgtcggctg gaaacacagt ccgctgcggc
     2281 attcgtcgtc ggaactgcag acgtcacctt cttcaagaag atccaccggc ggctcgccag
     2341 gcaggcatgc cccggccgaa agctcggttt cacaatacca gccgtccgca caattggcgg
     2401 aggttccaca agcttcccgg caccgctttc cgtcgcttcc ctgcaagcac aggccagtcc
     2461 tgcaaagcga aggaagcgaa cattcatccc cgacctcaag agtcggctcg accggctcct
     2521 gaccaggcag acaggtactg gactgttcat cgcaaacgaa cccttcgccg cacttgtcac
     2581 tgtccgtgca gaccaccctg caacgcattc catcgtcgcc aaacacgcac ttgccgcttt
     2641 cgcactgatc cccggcggtg cagtactcgc cggttccctt cagcacaacc tcaacttccg
     2701 gaaagcagcc cccgtaagat cccttgaacc agcagtactc gccatcgggg cattccttgt
     2761 cgctgcggca cattatccgg caagacctga cgccgtccgg ccacgtgtcg cacttgccct
     2821 cgacgcagtc atcactgttc gaacaacgac ttccaatacc gccggccttg ctgccggtca
     2881 cgcagatatc ctgcccgccc atcgtctcgc agtggtatcc cgagggacac aaatcgttcg
     2941 ccaggctgca ctcctcggta catcgcggct tgccgtcgcc gatgtcggtg cagatgccgc
     3001 ccttgcaggc aacatcgttc tgacaaatca caccctgaag agtggttccg ggctggcttc
     3061 catacaaccc gat
//
LOCUS       DAOPPA010000402         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34633_length_3072_cov_3.894597, whole genome shotgun sequence.
ACCESSION   DAOPPA010000402 DAOPPA010000000
VERSION     DAOPPA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34633_length_3072_cov_3.894597"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKH54_11215"
     CDS             <1..358
                     /locus_tag="PKH54_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOD00506.1"
                     /translation="GQYMYSDGENYFFMNQESYEQISIPASVLGDSALFLTENMECTI
                     LVFEGVPMNVALKTFLEAEIVYTEPGMKGDTATGGSKPAKISTGAEVQVPLFINQGDW
                     IKVDTRDGSYVERVKK"
     gene            443..1399
                     /gene="epmA"
                     /locus_tag="PKH54_11220"
     CDS             443..1399
                     /gene="epmA"
                     /locus_tag="PKH54_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOD00507.1"
                     /translation="MSDHVRFFDGKPSLFQNIPVRARCLALLREFFTARGFIEVETPI
                     VCTSPGVETHLAAMPVSCMNRTMYLTTSPEFHMKRLISVGFDRVFQVTRAFRDGERGE
                     RHNPEFTMVEWYRRDCDYGAIMDDSQELLRHVAMGIGGRLEFPGIEGVRGPISLENDF
                     RRVTMIQAFAELGLPSPFGMTLEERDQVAVDHLDFHLGRHEPEFLIEYPADQASLARK
                     KPEDPRVAERFELYCGGLELENGFSELADADEYLERCRIDLAERARLGLPQYPIDERY
                     VSMLRDGMPVCAGTALGFDRLVMLACGARTIRDVIAFPWDQA"
     gene            1505..2716
                     /locus_tag="PKH54_11225"
     CDS             1505..2716
                     /locus_tag="PKH54_11225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOD00508.1"
                     /translation="MLMTHLACNNILILATAALALTAMIGSNPAVASTRDSTLESELL
                     ARSDSVVIGTILAATPRRQTEGRIVTDYRVRVDSVEAGSMKDPATRNITVSLPGGTID
                     DDGQRVFGIPILIPGDIVRLFLRNPDGATPIGDAYLVTGLGDGIRLLARNADAFTFYS
                     LSSNPDVALRWYADTIDVHIDDQLTDDIDDAATLEAIQASLDEWNAVDCEHPTLVNVG
                     LVNGFDAITGSGAGIHATNLIIFENTAQWDENRQDVDQWDLDTTIALTTIFHNPRTGE
                     VLNFAIEFNDERFGFAVTGIPAAVDLENTLTHELGHVFGLDHTGQDIEQYWNQTMYFQ
                     AQVGETKKRSLEEDDINGLCTLYETDWSLNAETGGCSSAAQAFPWTTPLPTLVMLGAV
                     LIIFCIRRRGY"
     gene            2718..>3072
                     /locus_tag="PKH54_11230"
     CDS             2718..>3072
                     /locus_tag="PKH54_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00509.1"
                     /translation="MSARFHKAVAAAATIFAVILAAPLAFGQEAFELQFNNRVLKGQG
                     KPSILVVANTNMQKVSVKLTRDDGKVINLKAPLIRFGLRHEFKFDQEPGSHKYVMDMS
                     WGGRKEPDRFEFDVVV"
BASE COUNT          655 a          916 c          884 g          617 t
ORIGIN      
        1 cggccagtac atgtacagcg acggtgaaaa ctacttcttc atgaaccagg aatcgtacga
       61 gcagatctcg attcccgcgt cggtcctggg cgattcggct ttgttcctga ctgaaaacat
      121 ggaatgcacg atcctggtgt tcgaaggcgt cccgatgaac gtggcgctga agaccttcct
      181 ggaagctgaa atcgtataca cagaacccgg catgaagggc gacacggcca ccggcggcag
      241 caagcccgcc aagatttcga ccggcgccga ggtccaggtt ccgctgttca tcaaccaggg
      301 cgactggatc aaggtcgata cccgagatgg cagctatgta gagcgcgtga agaagtgatt
      361 tggccgctgg cggccggcaa cggtcccagt tggaccaacc agccgaagcc ccaccctgtt
      421 tctgcagcac caggtattgc tgatgtctga tcacgtgcgc ttttttgatg gcaagccatc
      481 cctttttcag aacattcccg tccgggcccg ttgcctggcc ttgctgcgcg aattcttcac
      541 ggcccgcgga ttcatcgagg tcgagacgcc catagtgtgc acttcccccg gtgtcgagac
      601 gcacctggcc gccatgcctg tttcgtgcat gaaccgcacg atgtacctga cgaccagccc
      661 ggaatttcac atgaagcgcc tgatatccgt cggattcgac cgcgtttttc aggtgacgcg
      721 ggcatttcgc gacggcgaac gcggcgaacg ccacaatccc gaattcacga tggtcgagtg
      781 gtaccggcgt gattgcgatt acggcgccat catggacgac agccaggaac tgctgcggca
      841 cgtcgcgatg ggcatcggcg gacgactgga atttcccggg atcgaaggcg tccgagggcc
      901 gatcagcctt gaaaacgact tcagacgcgt cacgatgatc caggcgttcg ccgaacttgg
      961 cctgccctcg cctttcggga tgactctgga ggagcgcgac caggtcgcag tcgatcatct
     1021 ggacttccac cttggacgac acgagccgga attcctgatc gagtacccgg cggatcaggc
     1081 ttcgctggca aggaagaagc ccgaagaccc gcgcgtggcc gaacgtttcg agctttactg
     1141 cggcggactc gaactggaaa acgggttttc tgaactggcc gacgcggacg aataccttga
     1201 acgatgccgc atcgaccttg ccgagcgtgc ccgcctgggg cttccccagt accccatcga
     1261 cgaacgctat gtgtcgatgc tgcgcgacgg aatgccggtc tgcgccggca ccgccctggg
     1321 ctttgaccgc ctggtcatgc tggcctgcgg cgcccgcacc atccgcgacg taatcgcctt
     1381 cccctgggac caggcctgat ggttcatggt cgtttcccgc caattgcccg tggtgcttaa
     1441 ccggaaggaa tcctatactc ggccgtacgg ctgatccggt tgttggacgg cttgatactg
     1501 ctggatgctt atgacccacc tggcttgtaa caacatattg attcttgcga cggcggcgct
     1561 ggctttgacg gcgatgatcg ggtcgaaccc ggctgtggct tcaaccaggg attccacact
     1621 ggaaagcgaa ctgcttgcca ggtcggactc tgttgtcatt ggaacgattc tggcggcaac
     1681 gccaaggcgg caaacggaag gacgcatcgt gaccgattac cgggtacgcg tcgattccgt
     1741 ggaggccggt tccatgaagg atcccgccac ccgcaacatc acggtttcac ttcccggtgg
     1801 gaccatcgac gacgacggcc agcgggtttt cggtattcca atactgattc ccggggatat
     1861 tgtcaggctg ttcctgagaa accccgacgg cgcaacccca atcggcgacg cctatttggt
     1921 aaccggcctt ggcgacggaa tccgactgct tgcgcgcaac gccgacgcct tcacttttta
     1981 cagcctgtca tcgaacccgg acgtggcgtt acggtggtac gccgacacga tcgacgtcca
     2041 tatcgacgat caactgaccg acgacatcga tgatgccgca acactggagg cgatccaggc
     2101 ttcactcgac gagtggaatg ccgtcgattg cgaacatccg acgcttgtca acgtcggact
     2161 tgtcaacgga ttcgacgcca ttaccggatc aggcgccggc attcacgcga ccaacctgat
     2221 cattttcgag aacacggctc agtgggatga aaaccggcag gacgtggatc agtgggatct
     2281 ggataccaca atcgccctga caaccatatt ccacaaccct cgaaccggtg aagtccttaa
     2341 ttttgcgatc gaattcaacg acgaacggtt cgggttcgcc gtgacgggca tcccggcggc
     2401 cgttgatctg gaaaatacat tgacccacga actcggacac gtcttcgggc tggatcacac
     2461 cggccaggac atcgagcaat actggaacca gacgatgtat ttccaggcgc aggtcgggga
     2521 aacaaagaaa agatcgcttg aagaagacga tatcaacggg ctttgcacgc tgtacgaaac
     2581 cgactggtcc cttaatgcgg aaacgggtgg ctgttcgtcc gcagcacaag cttttccatg
     2641 gacaacaccg ctgccaaccc ttgtcatgct tggcgcggtt ctgataattt tctgcatccg
     2701 ccggagaggt tattgaaatg tccgccagat tccacaaagc agtcgccgcc gcggcaacca
     2761 tcttcgccgt catccttgcg gcacccctgg cattcggcca ggaggcgttc gaactccagt
     2821 tcaacaaccg cgtgctgaag ggtcagggaa agccttcaat cctcgtggtg gccaatacca
     2881 acatgcagaa ggtctcggtc aagctgaccc gagacgacgg caaggtaatc aacctgaagg
     2941 cgccgctcat ccgtttcgga ctgcggcacg aattcaagtt cgaccaggag ccgggcagcc
     3001 acaagtacgt tatggacatg tcctggggtg gccgcaagga accggatcgg ttcgaattcg
     3061 acgttgtcgt cg
//
LOCUS       DAOPPA010000403         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34878_length_3056_cov_4.379207, whole genome shotgun sequence.
ACCESSION   DAOPPA010000403 DAOPPA010000000
VERSION     DAOPPA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34878_length_3056_cov_4.379207"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKH54_11235"
     CDS             <1..569
                     /locus_tag="PKH54_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD00510.1"
                     /translation="VVGYFDDLKWGGLVPGTALNLPEPPFPRFELPEFETRPAEAPAK
                     ADKAVADRANAAGGAKAEKAAGSQPSDGSSGAVASEHATFDDFLKIKLLVGTVLTAEP
                     VPGSDKLLKLSVDLGEPAPRQIVAGIGDRFPVEKVPGSQVTVVANLKPRKVFGIMSEG
                     MILAATDENGLSLIAPSGTPRKPGTRVG"
     gene            complement(585..914)
                     /locus_tag="PKH54_11240"
     CDS             complement(585..914)
                     /locus_tag="PKH54_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00511.1"
                     /translation="MCCFLTPMAAAAVATVARKKFPEKLHAGWLLMMLWGAVVALVVD
                     HAINGEISVYPPFLTALKSPEQTEIMLREIAVTGTAMTVAVFAFWGVSVAVANIRAKT
                     ARGNQPA"
     gene            complement(916..2685)
                     /locus_tag="PKH54_11245"
     CDS             complement(916..2685)
                     /locus_tag="PKH54_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD00512.1"
                     /translation="MKRIGILTQIIAFFLMAAPAALAAFPTAPAAPTAQLSEPDDSDP
                     EDIDYWKDVPFTDQDFASVRRIVEIQYIDPEYNRKLAWINAADYVLHTLEPPMTLMPR
                     TYFRIHHRDQAETRLNRRDRFVLTEHPKDDKVNFRSMTDDDIRKKKQQIRARAKKREA
                     EWRLIDFSEPDFRRVLDFVQEKSKDKAGFRVDDLYIAATSGYLASLDPHTSIVSAKAW
                     EESTRETEDGSFEGIGAMLTTRQDEIIVETPIEGQPAEKAGVRAGDQIVSVNGRKVTG
                     MSLQRVVKMIKGEKGTTVVLVVRRLGMPQDLEFRIVRQHIEVLNIQAQMLKGHPDIGY
                     MKITGFIDGTARRVQDAIAGLIRESRGGRLRGLVIDVRGNSGGLLQESVNIADAFLED
                     GVIVSVKSPKERDDVYRAKPGSWDFPVVVLTNSSSASASEILASAIQENRRGLVIGDR
                     TFGKASVQTLMPAYPRSDYFVKVTIARYYAPSGRTIQVTGVSPDIDAPPQFGKTQPLG
                     FREEDLHHHLTMLDQDHLPANAALVRRLSSCTAKMGTAGPKGLADLHPTIKADNQLAT
                     AADYLECMWLDREHNRKLSGGGM"
BASE COUNT          550 a          932 c          904 g          670 t
ORIGIN      
        1 ccgtggttgg atattttgac gatctgaagt ggggtggcct ggtgccgggt accgcattga
       61 acctgcccga gccgccgttt ccccgcttcg aacttcccga attcgaaacg cgtcccgccg
      121 aagcgcccgc gaaggccgac aaagccgttg cggatcgggc aaatgcggcc ggcggcgcga
      181 aagccgaaaa ggccgcgggt tcacagccgt cggacggttc gtccggggcg gtcgcatcgg
      241 aacacgcgac gttcgatgat ttcctgaaga tcaaattgct ggtcggcacc gttcttacgg
      301 ccgaaccggt tccaggctca gacaagctgt tgaagctgtc agttgatctg ggtgagcctg
      361 cgccccgcca gatcgtggcc gggattggtg acaggttccc ggtcgaaaag gttccgggca
      421 gccaggtgac cgttgtcgcc aacctgaagc cgcgcaaggt gttcgggatc atgtcggaag
      481 gaatgatcct ggcggccacc gatgagaacg gactgtccct gattgcgccg tccggcacgc
      541 cccgcaagcc gggaacccgc gtcggatagg gcacgggcgg attgtcaggc gggctgattg
      601 cctctggccg ttttggcgcg gatgttcgcc accgccaccg aaacacccca gaatgcaaag
      661 accgcgaccg tcatggccgt accggtcacg gcgatttcgc gaagcatgat ctccgtctgt
      721 tccgggcttt tcaaggcggt caggaacggc ggataaactg aaatttcgcc gttaattgcg
      781 tgatcgacta ccagcgcgac cacggcaccc cacagcatca tcagaagcca gcctgcgtga
      841 agtttctcgg gaaacttctt ccgtgcaacg gtcgcgacgg ccgcagccgc cattggggta
      901 agaaagcagc acatttcaca ttcctccgcc ggagagtttc cggttgtgtt ccctgtcaag
      961 ccacatgcat tccaggtagt ccgccgccgt tgcaagctgg ttgtcggcct ttatcgtcgg
     1021 atgaaggtcg gcaagtccct tcggtcccgc ggttcccatt ttcgccgtgc acgaggaaag
     1081 acgcctgacc agcgcggcat ttgccggcag gtgatcctgg tcaagcatcg tcaggtggtg
     1141 gtgcaggtct tcttccctga atccaagcgg ctgcgtcttg ccgaactgcg ggggcgcatc
     1201 gatgtcgggc gataccccgg tgacctggat tgtccgcccc gatggcgcgt agtaccttgc
     1261 gattgtcacc ttcacgaaat agtcgcttct tggatacgcc ggcatcaacg tctgaaccga
     1321 tgcctttccg aatgtgcgat cgccaatcac cagcccgcgc ctgttttcct ggattgcgct
     1381 tgcaaggatc tcggacgctg acgccgagga agaattcgta agcaccacga ccgggaagtc
     1441 ccagcttccc ggcttcgcgc gatacacgtc gtcccgttcc ttcgggcttt tgaccgatac
     1501 gatgacgcca tcctccagga aggcgtcggc gatgttcacg ctttcctgca gcaggccgcc
     1561 cgagtttccg cggacgtcta tgaccaggcc gcgcagccgg ccgcccctgg attcccgtat
     1621 cagccccgcg attgcatcct ggacccttct ggccgtgccg tcgatgaacc cggttatctt
     1681 catgtaaccg atgtcgggat ggcccttcag catctgggcc tggatgttaa gcacctctat
     1741 gtgctgcctg acgatcctga attccagatc ctgcggcatg cccaggcgcc tgacgacaag
     1801 gacgacggtt gttccctttt cgccttttat catcttgacg acgcgctgaa gtgacatccc
     1861 ggtgaccttc cggccgttga cggaaacgat ctggtcgccg gcccgcacgc cggccttttc
     1921 ggctggctgc ccctcgatcg gggtttcgac gatgatttca tcctgcctgg tcgtaagcat
     1981 cgccccgatg ccttcgaagc ttccgtcctc ggtttccctg gtcgattctt cccaagcctt
     2041 cgccgacacg atcgaagtgt gcggatccag ggaagccaga tagccgcttg tcgccgcgat
     2101 ataaagatcg tcaaccctga agccggcctt gtcctttgat ttttcctgga cgaaatccag
     2161 gacgcgtctg aaatccggtt cggaaaagtc gatcaggcgc cattccgcct cgcgcttctt
     2221 cgcccttgcc cggatctgct gcttcttctt ccggatgtcg tcatcggtca tcgacctgaa
     2281 attcaccttg tcgtccttag ggtgctcggt caggacgaac ctgtccctgc ggttcagcct
     2341 ggtttcggcc tgatccctgt gatgaatgcg gaagtaagtc cgcggcatca gggtcatcgg
     2401 cggttcaagg gtgtgcagca catagtcggc agcgtttatc catgccagtt tccggttgta
     2461 ctcggggtcg atgtactgga tttcaacaat tcttctgaca gaagcaaaat cctggtcggt
     2521 gaatggaaca tccttccagt agtcgatgtc ttccgggtcg gaatcgtccg gctctgacaa
     2581 ctgggcggtc ggcgcggccg gggccgtcgg gaaagccgca agcgcggccg gggcggccat
     2641 caggaagaac gcgatgatct gcgtcaggat gccgattctt ttcactcggg gcccccggaa
     2701 tcgattgtcg gcattatagc cgttttcgtc caaacaccgg agcaccgaaa agattccacg
     2761 gcgtgccgcg tgaacgcaag ggaacgcagc cgtcgagaca tggatgtcgg caggtgcccc
     2821 gcatgccgcc tccgacctcg gggccggtgg gagtcccgta ccgcctgaac agtgccgact
     2881 gtcctgactg tgagtttact tcgcttgtgc tagaatgcgc tcgtttcagc cccttgaagg
     2941 tggtgtcggg atggcccgtg gtcagtttgc cgcatacctg aatgcaaacg ccaagcgcat
     3001 gtcgcccgaa gtcgtttcgg cggttcagga actgatttat cccgacgaca tattct
//
LOCUS       DAOPPA010000404         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34965_length_3050_cov_3.782972, whole genome shotgun sequence.
ACCESSION   DAOPPA010000404 DAOPPA010000000
VERSION     DAOPPA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34965_length_3050_cov_3.782972"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3050)
                     /locus_tag="PKH54_11250"
     CDS             complement(<1..>3050)
                     /locus_tag="PKH54_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOD00513.1"
                     /translation="QAGDIHFQGAAVTGSGKDLVSGSIGAVVINEIVAHATGAWTIRP
                     DVASIIEIGGQDSKFIQVGRDAGGSHYLKDHAFNELCAAGTGAFLDQQAARLGLDLDT
                     FSTQAAAAARPARLSGRCSVFAKSDMIHLQQKATPVGEIAAGLCFALARNYLATLCRG
                     RHPQPPILFQGGVASNAGVVRAFREILALDDQALVVPPDHDVMGAFGAAVHSRSRPFE
                     GGPIGLASMVERLRAIPAPGPEPSGLEPLRRPESTFLECEGEPGEGPFFLGLDIGSVS
                     TKGVVVDSAARCVARSYLPTSGRPVEAVRAVLGELRDACGKACRMQAVCCTGSGRYLA
                     SALVGGGAVMDEISAQTAGAAHFFEDVDTIIEIGGQDSKYIKVSNGRVERFQMNRACA
                     AGTGAFLQEQSGRLGIDIRRDFAEMAFRSDNPVKLGTRCTVFMDSDLVHHVQRGTPTA
                     DICAGLAYSIASNYLEKVVGSRPIGSVVLFQGGVARNQAVHAVFSNLLKRKVVVHPMP
                     EVSGAFGAALAAMREPGARLDFDLAVPPGEAVTDTFECRSCANICEIRRVTLADGRRA
                     FFGSICGRFDRREETPSEPVDAFAERERLLFFHASIESGADSPAPLGTIGIPFALSMT
                     DYLPFWNTFLRHVGFRTVLSGPSSRDIVEWGLAHVPGEFCYPVKVLFGHVHSLMARGI
                     ERLFIPHLRMFTPPGESVSRYACAYTQAAPYVVMQNTDADVLALEYPVQDETVWWIKD
                     VARKLGLDVGLVREAWRQADQAQSAFVQACRNAGRQIIDRLKVEGGRGCVVLGRPYNT
                     TDRHVNLNLARRLSALDIEPIPFDFMPLGDSPLPPLWGRVRWGYGRKLLQAARLLKDH
                     PNLGAVILTNFGCGPDSFVDQYLETELSGVPHIALELDDHQAEAGLVTRLEAFSRTFR
                     IRRSDAAPPELSMGVPGEPRRPLREYTYYIPSFVDHSHAFAGALRAAGCRVVLLPPTD
                     DESWELGLQHAYGRECHPFISFAGDLLKAARRPDFVAAEACYFGP"
BASE COUNT          623 a         1007 c          935 g          485 t
ORIGIN      
        1 ggcccgaaat agcacgcctc ggccgccacg aaatcggggc gcctggccgc cttcagcagg
       61 tcgccggcaa acgatatgaa gggatggcat tcacggccgt atgcgtgctg cagaccgagt
      121 tcccagcttt cgtcatcggt cggcggcagc agcacaaccc ggcacccggc cgcgcgcaat
      181 gcccccgcaa aggcgtgcga atgatcgacg aaagacggaa tgtagtatgt gtattcccgc
      241 aacggacgcc ggggctcgcc gggaacgccc atcgacaact ccggcggggc ggcgtcggac
      301 cgcctgatcc tgaacgtgcg ggaaaaggct tccagcctgg tgaccagccc ggcctcggcc
      361 tgatggtcgt caagctccag cgcgatgtgc gggacgccgg aaagttcggt ttccagatac
      421 tggtcaacga acgaatccgg cccgcacccg aaattggtca gaatgaccgc accaagattc
      481 ggatggtcct tcaacagcct tgccgcctgc agcagcttgc gcccgtatcc ccagcgcacg
      541 cggccccaga gcggtggaag cggcgaatcg ccaagcggca tgaagtcgaa cggtatcggc
      601 tcgatgtcca gcgcggaaag ccgcctggca agattcaggt tcacatgccg atcggtcgtg
      661 ttgtaggggc gtcccagaac gacgcagcca cgaccacctt caaccttaag cctgtcgatg
      721 atctggcgcc ccgcgttccg gcaagcctgg acgaacgccg actgggcctg gtcggcctgc
      781 cgccacgcct cgcgcaccag gccgacgtca aggccaagct tccttgcgac atcctttatc
      841 caccagacgg tctcgtcctg caccgggtac tccagcgcca gcacgtccgc atccgtgttc
      901 tgcatgacaa cgtacggtgc ggcctgggtg tatgcacagg cgtatcgcga aacgctctcg
      961 ccgggcggcg tgaacatcct caggtgcggg atgaacagcc tttcgatgcc gcgggccatc
     1021 agcgaatgca cgtggccgaa aagcaccttg accggataac agaattcacc gggtacatgg
     1081 gccagtcccc attcgacgat gtcgcggctg cttggcccgg aaagcacagt ccggaacccg
     1141 acgtgccgaa ggaacgtgtt ccagaacgga aggtaatcgg tcatgctcag ggcaaaaggt
     1201 attccaatgg tacccagcgg cgccggagaa tcggcgccgg attcaatcga cgcatggaaa
     1261 aagagaagcc gttccctttc ggcaaacgca tccaccggtt cggaaggcgt ctcctcccgg
     1321 cggtcgaagc gcccgcagat gcttccgaag aaggcccggc gcccgtccgc cagcgtcacc
     1381 ctgcgtatct cgcagatgtt cgcgcacgaa cggcattcga aagtgtcggt gaccgcctcg
     1441 cccgggggaa ccgcaaggtc gaaatccagc cttgcgcccg gttcccgcat ggccgcaagt
     1501 gccgcaccga atgcgcccga aacttccggc atcggatgga ccacgacctt tcgtttcagc
     1561 agattggaaa agaccgcatg aaccgcctgg tttcgggcga ccccgccctg gaaaagcacg
     1621 acgcttccaa tcggacgcga tccgacgacc ttctcgagat agttggaggc gatcgagtat
     1681 gccagtccgg cgcagatgtc ggcagtcggg gtgccacgct ggacgtgatg cacaaggtcc
     1741 gaatccatga agacggtgca ccttgtgcca agcttgaccg ggttgtccga ccggaaggcc
     1801 atttcggcaa aatcacgacg gatgtcgatg ccaagacgcc ccgactgttc ctggaggaag
     1861 gcgccggttc cggccgcgca ggcacggttc atctggaacc gctcgacccg gccatttgaa
     1921 accttgatgt atttcgaatc ctggccgcca atctcgatga tggtatcgac gtcttcaaag
     1981 aagtgggcag cgcccgccgt ctgggccgaa atctcgtcca tgacggcccc gccgccgaca
     2041 agagccgacg caagataccg cccggaaccg gtacagcaga cggcctgcat cctgcatgcc
     2101 ttgccgcacg cgtccctcaa ctcgcccagc acggccctga ccgcctcgac tggacgaccg
     2161 gacgtgggaa gatatgaccg cgcgacacac cttgcggcgg aatcgacgac aacgcccttg
     2221 gtgctgacgg aaccgatgtc caggcccagg aaaaaggggc cttcgccagg ttcgccctcg
     2281 cactcgagaa acgtcgattc agggcgccgc agtggttcca ggccggatgg ctcgggaccg
     2341 ggggccggaa tcgcgcgaag acgttcgacc atcgatgcaa gcccgatcgg gccgccctca
     2401 aacggccttg acctcgaatg aaccgcggcg ccgaaagcac ccatgacgtc gtggtcgggc
     2461 ggcacgacca gcgcctgatc gtcaagggcc aggatttccc ggaacgccct gaccacgccg
     2521 gcattcgagg cgaccccacc ctggaagagt atcggcggct ggggatgccg ccccctgcac
     2581 aaggtcgcaa ggtagttcct ggccagcgcg aaacacaggc cggcggcgat ctcgccgaca
     2641 ggcgtggcct tctgctgaag atggatcatg tcggacttgg caaacaccga gcaacgcccg
     2701 gaaagccttg ccggccgggc ggccgcggcg gcctgggtcg aaaacgtgtc caggtcaagc
     2761 ccaagccggg cagcctgctg atctagaaac gcgccggtgc cggcggcgca caactcgtta
     2821 aatgcgtgat ccttaagata atgggatccg ccggcgtccc tgccgacctg gatgaacttg
     2881 gaatcctggc cgccgatttc gatgatgctc gcgacgtccg gccttatcgt ccaggcgccc
     2941 gtcgcatggg ccacaatctc gttgattacg accgcgccga tcgaaccgga aaccagatcc
     3001 ttgcccgaac cggtcacggc cgcgccctgg aaatggatat cccccgcctg
//
LOCUS       DAOPPA010000405         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_34984_length_3049_cov_3.252839, whole genome shotgun sequence.
ACCESSION   DAOPPA010000405 DAOPPA010000000
VERSION     DAOPPA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34984_length_3049_cov_3.252839"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="PKH54_11255"
     CDS             <1..2125
                     /locus_tag="PKH54_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00514.1"
                     /translation="MCDECAPGYWGDECDPCPNCEMGQCNEGDDGDGTCVCDPGWDGT
                     LCDICADGFFGLICDPCPECVHGICDDGIFGGGKCYCEPGYAGDLCDDCVAGHFGNSC
                     QECPDCFAGTCADGIRGSGTCDCDDGWVGPLCNRCNDGFFGAFCLACPDCGHGICIDG
                     SEGSGECSCDPGWAGADCTACAPGYYGSGCDECRNCVNGTCRDGHEGDGTCACDEGWA
                     GPDCSDCVAGRFGPDCLPCPACVNGTCSQGVIGTGKCDCKPGFTGLLCDACEEGHFGA
                     GCTACADCGSGTCIDGIEGDGSCECPEGWVGQRCTECESGYFGPACSMCPECVHGTCR
                     DGIEGDGACECDDFWDGILCNSMFCEPECGNNATCHEANECRCNEGFSGDGYVCEDTN
                     PCAGKDDWTSCDGGYCFDETCTALPEGDECEAATELEVGAEMAGSLAGFRPAVTNSII
                     CQVAVENGPDRWFMFHVTEGLKYRVELTTLTFNAVMQVMDTCHSSSACIANADEDDGA
                     GIESATFTAASGGYLVVRVAAVQDSVTSGDFVLKVEEVQEPVDNSTSPEESADEAVAD
                     VIEDVGQEDASDDDASAEDMAAEDAGDVSTGDLNATDTNVQDLSEPDISAPDTSLPDN
                     AGDTASQDTAVTPDPGRDEGNDTAVSQDESNVNDVTADDGAEEIGLGGGGSCSAGGSA
                     GSSSAALVLLLLVFCAFVMRRRRVA"
     gene            complement(2549..>3049)
                     /locus_tag="PKH54_11260"
     CDS             complement(2549..>3049)
                     /locus_tag="PKH54_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00515.1"
                     /translation="VSFKQASDFCAWSGKRLPTEAEWEFAARGDDGRKFPWGDENATC
                     ALAVMDGVDGVDCGEGTDKPVCVGCGTGSTVKVCSRSPAGDSPFGLCDMAGNAWEWVS
                     DWYGKYPKDAVTDPKGPDSGEFHVSRGGGIYDVMANLRTTNRYDADRMVTNGFDTGFR
                     CAGDAD"
BASE COUNT          605 a          948 c          944 g          552 t
ORIGIN      
        1 aatgtgcgac gaatgcgcgc ccgggtactg gggcgatgaa tgcgatccct gccccaattg
       61 cgaaatgggt caatgcaatg aaggcgacga tggagacggc acctgcgtct gcgatccggg
      121 ctgggacgga accctgtgcg atatatgcgc ggatggattc ttcggcctga tttgcgaccc
      181 ctgccccgaa tgcgtgcatg gaatctgcga cgacggcatt ttcggtggcg ggaagtgtta
      241 ctgcgaaccg ggatacgctg gcgacctttg tgacgactgc gttgccggac acttcggcaa
      301 ttcctgtcag gaatgccccg actgcttcgc cggaacctgt gccgatggaa tccgcggcag
      361 cggaacgtgc gactgcgatg acggatgggt cggcccgctg tgcaaccgct gtaacgacgg
      421 gttcttcggc gccttctgcc tggcttgccc cgactgcggc cacggcatct gcatcgatgg
      481 cagcgaaggc tccggtgaat gcagctgcga tccgggatgg gccggcgccg actgcactgc
      541 ctgtgccccc ggatactacg gatcgggatg cgacgaatgc agaaactgcg ttaacggcac
      601 gtgcagggat ggccacgaag gtgacggaac ctgcgcctgc gacgaaggct gggccggacc
      661 cgactgttcc gattgcgttg ccggccgctt cggccccgac tgcctgccct gtcccgcgtg
      721 cgtgaacgga acctgcagcc agggcgttat tggaaccggc aaatgcgact gcaaaccggg
      781 attcacggga ctgctttgcg acgcctgcga agaaggccac ttcggtgccg gctgcaccgc
      841 ctgcgccgac tgtggcagcg gaacatgcat cgacgggatt gaaggcgacg gctcgtgcga
      901 atgcccggaa ggatgggtcg gccagcgctg caccgaatgc gaatccggct acttcggacc
      961 ggcttgcagc atgtgcccgg aatgcgttca cggaacgtgc cgtgacggga tcgaaggcga
     1021 cggcgcgtgc gaatgcgacg atttctggga cggcattctg tgcaacagca tgttctgcga
     1081 gcccgaatgc ggtaacaacg ccacgtgtca cgaagcaaac gaatgcagat gcaacgaagg
     1141 gttcagtggc gacggatatg tctgtgagga tacaaacccg tgcgccggca aggacgactg
     1201 gacgtcctgc gacggtggat actgctttga tgaaacctgc acagcgcttc cggaagggga
     1261 cgagtgcgaa gccgcgaccg aattggaggt cggcgcggaa atggctggct cgctggccgg
     1321 attcaggccg gcagtgacga acagcatcat ctgccaggtg gcggtggaaa acggacccga
     1381 tcgctggttc atgttccatg tgaccgaggg actcaaatac agagtcgaac ttacgactct
     1441 gaccttcaat gccgtcatgc aggtgatgga cacctgccat tccagttccg catgcatcgc
     1501 caatgccgac gaggacgacg gcgccggaat tgaatcggca acatttacgg ccgcttccgg
     1561 cggatatctt gtcgtccgcg ttgcggctgt ccaggattcc gtcacttccg gtgatttcgt
     1621 ccttaaggtg gaagaggtcc aggaaccggt tgacaacagc acttcgccgg aagaatcggc
     1681 cgacgaggcc gtcgcggacg taatcgagga cgtcggccag gaagatgcca gcgatgatga
     1741 cgcatcagcc gaagacatgg cggcagaaga cgccggcgat gtgtcaaccg gggatcttaa
     1801 tgccacggac acgaatgtgc aggatctgtc ggaaccggac atatcggccc ccgacacgtc
     1861 cctgcccgac aacgccggcg acaccgcgtc gcaggatacg gcggtaaccc ccgatcccgg
     1921 tcgggacgaa ggcaacgaca cggccgtttc ccaggacgaa tcgaacgtca acgacgtgac
     1981 cgccgacgac ggcgccgaag aaatcggcct gggtggcggc ggttcgtgct ccgccggagg
     2041 ttccgccggc tcttccagtg ccgcgcttgt ccttctgctg ctggtcttct gtgcgttcgt
     2101 gatgcgtcgc cgcagggttg cctgaacaat cccgtaccca tccggccaca aggaaacagc
     2161 gccgtccgcg ctgccgtccg cgctgccgtc cgccccacac atgaaatttt ccgggacggt
     2221 gcccgcgaag tccgccgtcc ccgtgaagtc cgccgtcccc gtgaagtccg ccgtccccgt
     2281 gaagtccgcc gtccccgcga agtccgccgt ccccgtgaag tccgcggtcc ccgtggagtt
     2341 cgccatccct gcagattcca ccgcccagtg gatcgtgccc tccgtgagaa gtccggcatc
     2401 ccccgggata tgacttcttt gagtaatggc acttcctgac tcggcgtacc gactcagaca
     2461 acgcccggag acaaccctgt cctgcaataa aagacgatgt aaaatcttga aaagaaacga
     2521 tgaagggatg ctgaaaaaga aaggtctatc agtcggcgtc gccggcgcag cggaaaccgg
     2581 tgtcgaatcc gttcgtcacc atccggtcgg catcgtacct gttcgtggtt cgaaggttgg
     2641 ccataacgtc gtaaatcccg ccaccacgcg acacatggaa ttcgccggag tccggtcctt
     2701 tcggatcggt cacggcatcc ttcgggtatt tgccgtacca gtcggaaacc cattcccatg
     2761 cgtttccggc catgtcgcac aggccaaacg gactgtcgcc ggcgggtgac ctgctgcaca
     2821 ccttcacagt actgccagtt ccgcagccga cgcataccgg cttgtcggtg ccctcgccac
     2881 aatcgacgcc atcgacgcca tccatcacgg ccagcgcgca cgtcgcgttt tcatcacccc
     2941 acgggaactt ccttccgtca tcgccgcgtg ccgcaaactc ccattcagcc tcggtcggaa
     3001 gtcgctttcc ggaccaggcg cagaaatccg atgcctgctt gaaagacac
//
LOCUS       DAOPPA010000406         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35226_length_3032_cov_3.769567, whole genome shotgun sequence.
ACCESSION   DAOPPA010000406 DAOPPA010000000
VERSION     DAOPPA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35226_length_3032_cov_3.769567"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1107)
                     /locus_tag="PKH54_11265"
     CDS             complement(232..1107)
                     /locus_tag="PKH54_11265"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOD00516.1"
                     /translation="MPDKSIESLDIGAHLTIAGGIPAAMKMAHEIGATNFQFFPRNPR
                     GGARRAISQSEMADGRAARVEYGIRTVICHLPYTVNPASPDERISDFARMVVAEDVPF
                     AAEMGADFVVLHPGAHMGDGPAAGIERVGRLFDNALAGYRGGARVLFEVMAGGGSVLG
                     STVSDIAGLIQAAGGHEWLGTCFDSCHLLAAGWDVTTPAGIDTMLAAFDAAVGLSRVG
                     CMHLNDSKTHKDSHKDRHELIGRGVIGESGLLALLSNPFIRALPLCLETPVDDIRDYD
                     SEIRQVRRIAALATV"
     gene            1467..>3032
                     /locus_tag="PKH54_11270"
     CDS             1467..>3032
                     /locus_tag="PKH54_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOD00517.1"
                     /translation="MKHFMVLAATLTMTLCIAPANATTGSDEGPGVIYAVGASHLDTQ
                     WWWTIQETIDELLAPTFTDTLDLLDKYPGFQFSWEGAFRYMLLKEYYPDVFFRTVPYI
                     ESGRWFPGGSAIDAGDVNIPSPESLIRQFLYGNRYFQKNFGKTSTDVFLPDCFGFGWA
                     LPSVAAHCGIRGFSTQKLEWGGAIPIPFGIGIWKGPDGASVLAALNPGSYTTQLEQDP
                     GLSPRWISALDAQEAVSGLRLAFMYFGVGDRGGSPGESSVALLETATAREEGPKVVVA
                     PSDQIFRDLDETEVAALPVYDGELIMRYHGTGSYTAQSAMKRYNRRNEQLADAAERAS
                     VIADWLGTRDYPTAALESEWIRFLWHQFHDDLTGTSIPEAYQFSWNDELLTMNRFTAI
                     LQDAVGATASLLDTRTDGLAVVVFNPLAFEREDVVATTLTVDSDWPGFVKVIGPEGRE
                     VPSQVTGRTPGKGGMDRLEIIFLANAPSTGFIVYDIRPSTTACQIDSGLKGNVDGLEN
                     HRYKVVFDTAGNIS"
BASE COUNT          644 a          909 c          911 g          568 t
ORIGIN      
        1 cgacaggatg tccatgaaga tgtacgaaga aaacatcgcc agcgaaagca gaagcagcac
       61 ggtccatctt gcggccgcgc tctccctcag gtctgccaat tgtctggtca cggtgaagac
      121 tcctgtaaaa gccgggtcaa tttgctgaga aacgcccatg gtgaactaaa ggcagagggt
      181 acaccaaaaa ccggtcgcgg caaccacaaa aaagagcccg cctgggaggc gtcaaaccgt
      241 cgccagggca gcgatccgac ggacctggcg aatctcggag tcgtagtcgc ggatgtcgtc
      301 aaccggcgtt tcaagacaca ggggaagggc gcggatgaag gggttcgaaa gcaacgccag
      361 caggccggat tccccgatta cgccgcgccc gataagctcg tggcgatcct tgtgggaatc
      421 cttatgggtc tttgaatcgt tcaggtgcat gcagccgacc cgggaaagtc cgacggccgc
      481 gtcgaaggcg gcaagcatcg tgtcgatgcc ggccggcgtc gtgacgtccc agcccgcggc
      541 cagcaggtgg cacgaatcga agcaggttcc aagccattcg tgtccgccgg ccgcctggat
      601 caagcccgcg atgtcggaaa cggtcgaacc aaggacggag ccgccgccgg ccatgacctc
      661 gaaaaggacc ctggccccgc cccggtatcc ggcaagggcg ttgtcgaaca ggcgccccac
      721 cctttcgatg ccggcggccg gaccgtcacc catgtgggcc ccggggtgca gcaccacgaa
      781 gtccgccccc atctcggcgg caaaaggaac atcctcggcc acgaccatgc gggcaaaatc
      841 ggatatgcgt tcgtccggcg acgccgggtt gacggtgtag ggaaggtggc agattaccgt
      901 ccggatcccg tattccaccc gcgccgccct gccgtctgcc atctcggatt gggatatcgc
      961 ccttctggcg ccgccacggg gattgcgcgg aaagaactgg aagttcgtcg cgccaatttc
     1021 atgcgccatc ttcatcgccg ccggaatccc gccggcaatc gtaaggtgcg ccccgatatc
     1081 aagcgactca attgatttgt ccggcatgaa aacactcccc tggaaacgac aacgggttca
     1141 gatcgacaag gatcgagcgt tactccttca ggaaaatatc atgaaatgtc gcgggtggta
     1201 ccaaattgca gattcatcac gcaagtcctt tacacggcaa cgcttccact ggcattcacc
     1261 ggcagggtgc aatcacggcg gcttcagtcc tgaacggccc gggcgcggct tgagtcggcg
     1321 ttctgccttt tcgcccctgt cggaaccaga ccgatgaccc gatcgattat cgacctcaaa
     1381 tgccagtctc tgcctgattt ccgattgacg gtgcgtccgg cgcaccccag aatgcgggat
     1441 tgtgggtctg atgaaaggat caatcgatga aacatttcat ggtgcttgct gcgaccctga
     1501 cgatgactct ttgcatcgcg ccggcgaacg ccactaccgg ttccgacgaa ggaccgggag
     1561 tgatctacgc ggtgggcgcg tcacatcttg acacgcagtg gtggtggacc atccaggaaa
     1621 cgattgacga attgctggcc cccacattta cggacacact ggatctgctg gacaaatatc
     1681 cgggattcca gttttcctgg gaaggagcct tcaggtacat gctcctgaag gaatactacc
     1741 cggatgtatt cttcaggact gttccctata tcgaaagtgg ccgctggttt ccgggtggaa
     1801 gcgcgatcga tgccggcgat gtgaacattc catcccccga atcgctgatc cgccagttcc
     1861 tttacggtaa ccgctatttc cagaagaatt tcggcaagac cagcacggac gtattcctgc
     1921 cggactgctt cggtttcggc tgggcactgc cgtccgtggc ggcccactgc ggaatcaggg
     1981 gattctcgac gcagaagctt gaatggggcg gcgccatccc gatacctttc ggaatcggca
     2041 tatggaaagg ccccgatggc gcaagcgtcc tggccgccct gaaccctggc tcctacacga
     2101 cgcagcttga acaggatccg gggctttcac cacgatggat ttcggcgctg gacgcgcagg
     2161 aagccgtttc cggactgcgc ctggcgttca tgtacttcgg cgtcggcgac cgtggcggat
     2221 cgcccgggga atcaagcgtg gcccttcttg aaacggccac tgccagggaa gaaggcccta
     2281 aggttgttgt cgccccgtcc gatcagatct tccgggatct tgacgagacc gaggtcgcag
     2341 cgctgccggt atatgacggc gaactgatca tgagatacca cggaaccggt tcgtacaccg
     2401 cccagtcggc catgaagcgt tacaaccgca ggaacgaaca gcttgccgac gcggccgaac
     2461 gcgcctcggt gatcgcggac tggctcggga caagggacta cccgacagcg gcacttgaat
     2521 ccgaatggat tcgcttcctg tggcaccagt tccacgacga cctgaccggc accagcattc
     2581 ccgaggcgta ccagttttcc tggaacgacg agttgctgac gatgaaccgt ttcaccgcca
     2641 tccttcagga tgcggtcggc gcgacggcat cattgctgga cacgaggacg gatggcctgg
     2701 cggtcgtggt tttcaatccc ctggcgtttg aacgcgagga tgttgtggcg acaaccctga
     2761 cggtcgattc ggactggccg gggttcgtga aggtcatcgg cccggaaggc cgggaagtcc
     2821 cgtcccaggt tacaggcagg acgcccggta aagggggaat ggacaggctg gaaatcatat
     2881 tccttgcaaa tgcgccatcg accggcttca tcgtttacga catcaggccg tcaacaactg
     2941 cctgccagat cgattcaggc ctgaagggca acgtggacgg gcttgaaaac catcgctaca
     3001 aggtggtctt cgacacggcc ggcaacatat cg
//
LOCUS       DAOPPA010000407         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35302_length_3027_cov_3.784657, whole genome shotgun sequence.
ACCESSION   DAOPPA010000407 DAOPPA010000000
VERSION     DAOPPA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35302_length_3027_cov_3.784657"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PKH54_11275"
     CDS             complement(<1..862)
                     /locus_tag="PKH54_11275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOD00518.1"
                     /translation="MDVFAGSGTVRPVPGRLIVRGRLARKAAAAGRKAGRRAPKPAAK
                     GIAGYFQSTKAVGISLVLTLPVLLLYNVSLIVPANDTINTVDLLSRIVGTRYGMYGLL
                     VANSIIGLVSLVLGVVLAKRGRLKWWYWALLVVEGAAWAVLVAAAGYWANTGLGQTVP
                     SASIMRPNANYSLMQILTFSAGAGYWEELVFRLGVIGVPFGLARWFSGRPGGSGGLMP
                     AVVAGVMLFVSAIVFSSVHYIGVQAHFDTWSFVFRAVAGLVFGLLFLFRGFSVAVFAH
                     FMYDIAAFWLG"
     gene            complement(811..1710)
                     /locus_tag="PKH54_11280"
     CDS             complement(811..1710)
                     /locus_tag="PKH54_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD00519.1"
                     /translation="MKIAHLTDLHIDSPEAITPGLLFTGPMPNRRLLGAANFLLNRRS
                     VHQVPVLQAAVRAVGETGADFCVITGDVSNLAIDAEFKFVRGILDGIGGPDKLSLVPG
                     NHDWYTPAAVKAGLFEKYFGDLIWRPGEVHGQWPVYRDFDGVRLILARTAWTPPPGMA
                     WGKMGPDQLDRIFRLSEEAADAGKFVILAQHHHMHRRAKLLHRTTGWYIDQKRELEMI
                     SRSRIGLVIHGHDHDHFDHVVPSRHASGGTRIICGGSATYMDQHHGRFGRMGVYEVRD
                     GVLVVEKWMYSPDRERFVQSLEG"
     gene            complement(1747..2742)
                     /locus_tag="PKH54_11285"
     CDS             complement(1747..2742)
                     /locus_tag="PKH54_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00520.1"
                     /translation="MAAKKHPSGYGNNSGPGGRKEPDKPAALVTRAEKIEAFLFEWGR
                     ITVPIVVFGTLGLLYLLGVLDKVGLGYGIGLSILLAPPIAVAFIVLRGYFPAWARITT
                     IVVTALYLAGSIWPFTNMVYPGVPEFSRDLTREQGEQKLPRDIRGGYYWVEVFGKSFS
                     DVAGVRNERGRYLVNLNGGKIAGEFSDQPISGNMGTFDGMMVSQRKAMRFDPGPMTLR
                     AVRVDSAIGPTVTVSGFWMPVPPTIFFAILAIALMWAVFVDSWFRNQTWRWQLAPWVG
                     ISLVFLAFFYWTWEPARMPTTAVWSGVIGGIGGFLAGWLMTVIGRAIIGKLRTKI"
BASE COUNT          646 a          997 c          874 g          510 t
ORIGIN      
        1 accccagcca gaatgcggca atgtcataca tgaagtgggc aaagacggcg accgagaagc
       61 cacggaagag gaacaggagt ccgaatacca gcccggcgac cgccctgaac acgaacgacc
      121 atgtgtcgaa gtgtgcctgg acgccgatgt aatggactga agaaaagacg atcgcggaaa
      181 cgaaaagcat cacgccggca acgaccgccg gcatcaggcc gccacttccg ccgggacgcc
      241 cggaaaacca cctggccagc ccgaagggaa ccccgatcac ccccaggcga aaaaccagtt
      301 cttcccagta tccagcgccg gccgaaaagg tcagtatctg catcaggctg tagttggcgt
      361 tcgggcgcat gatgcttgcg gaaggaacgg tctggcccag tcccgtgttg gcccagtaac
      421 cggcggcggc gacaaggacc gcccaggcgg cgccctcgac tacaagcagg gcccagtacc
      481 accacttcag gcggcccctt ttggcaagca cgacgcccag gaccagggaa accagcccga
      541 tgatggaatt cgcgaccagc aggccataca tgccgtacct ggttcccacg attcgggaca
      601 gaagatcgac cgtgttgatg gtgtcgttcg cggggacaat caggcttacg ttgtaaagaa
      661 gcagcaccgg cagcgtcagg accagggaaa tcccgacggc cttcgttgac tggaaatatc
      721 cggcaatgcc cttcgccgcg ggcttcggtg cccgacgacc tgccttcctt ccagcagccg
      781 cggccttgcg cgccagcctg ccccggacaa tcagccttcc agggactgga cgaaccgttc
      841 ccgatccggc gaatacatcc atttttccac cacaagcacg ccgtcgcgga cttcgtacac
      901 gcccatcctg ccgaacctgc cgtgatgctg gtccatgtag gtcgcagatc cgccgcagat
      961 gatgcgtgtc ccgcccgaag cgtgccgcga aggcacgacg tgatcgaaat ggtcgtgatc
     1021 gtggccgtga atgaccagcc cgatgcgcga ccgcgaaatc atttccagtt cccttttctg
     1081 gtcgatatac cagcccgtgg tcctgtgcag cagcttcgcg cgcctgtgca tgtggtgatg
     1141 ctgggccagg atgacgaact tcccggcgtc ggccgcctct tcggacaggc ggaatatgcg
     1201 gtcgagttgg tccggcccca tctttcccca cgccatcccg ggtggaggcg tccacgcggt
     1261 ccgcgccagg atcagcctga cgccgtcaaa gtcgcggtac accggccact ggccatggac
     1321 ttcccccggc cgccagatca agtccccgaa gtacttttca aacagccccg ccttgacggc
     1381 cgccggcgtg taccagtcat ggtttccagg caccagtgaa agcttgtccg gcccgccgat
     1441 cccgtccagg atgccgcgca cgaacttgaa ttcggcgtcg atcgccaggt tcgagacgtc
     1501 cccggtgatc acgcagaaat cggcccccgt ctcgccaacc gccctgaccg cggcctgcag
     1561 gaccggcacc tggtggaccg agcggcggtt cagcaggaaa ttcgcggcgc ccagcagacg
     1621 acggttcggc atcggaccgg taaacagcag ccccggcgtt atcgcctcgg gcgaatcgat
     1681 atgcagatcg gtaaggtgcg caatcttcat ggatcctccg ggatgaatgg taacacgggg
     1741 cgggcgtcag atcttagtcc gcagctttcc gatgatcgcg cggccgataa cagtcatcag
     1801 ccaaccggcc aggaagccgc ctataccgcc gatcacgccg ctccagacgg cagtcgtcgg
     1861 catgcgggcc ggttcccagg tccagtagaa gaaagccagg aacaccagtg aaatcccgac
     1921 ccacggggca agctgccacc gccaggtctg gttccggaac caggaatcga cgaacaccgc
     1981 ccacatcagg gcaatcgcca ggattgcaaa gaagatggtg ggcgggaccg gcatccagaa
     2041 accggaaacc gtgacggtcg ggccgatggc ggaatcgacg cgcacggcac gcagggtcat
     2101 cgggccggga tcaaaccgca tcgccttgcg ctgggacacc atcatcccgt cgaaagtgcc
     2161 catgtttccg gaaatcggct ggtcggagaa ctcgccggca atcttgccgc cgttcaggtt
     2221 gaccaggtac cgaccgcgct cgttcctgac accggccacg tcggaaaacg acttcccgaa
     2281 tacctcgacc cagtaatacc cgccgcggat gtcgcggggc agtttctgtt cgccctgctc
     2341 gcgggtcaaa tccctggaaa actcgggcac gcccggatag accatgttgg tgaaaggcca
     2401 gatggatccg gcaagataca aggcggttac gacaatggtc gtgatgcgcg cccaggccgg
     2461 aaagtacccc cgaagaacga tgaacgccac ggcgattggg ggggccagca gaatcgacag
     2521 gccgattccg taaccaaggc cgaccttgtc cagcacacca agcaggtaaa gcaggccgag
     2581 cgtgccgaac accacgatcg gaaccgtgat gcgcccccat tcgaacagga atgcttcaat
     2641 cttttcagcc ctggtgacca gcgccgccgg cttgtccggc tccttgcgcc cccccggccc
     2701 actgttgttc ccataacccg atggatgctt cttcgccgcc atttttcagt cctatacgcc
     2761 gtttataacg atcaactttc gcgggatcgt acccgtacgc cgccgtcccg tcaatttggg
     2821 gctgcgagcc tgtcgcggac gtgattcaca tgtgacatgg gttcttttgc cacgaagaaa
     2881 catcaattga tgatgagaac tgttttgtga gccgtcttta tctctgccac caccacagct
     2941 tccgcctcgg cacgcttcgc cgccccgcca cgcttcagcc aggcataaaa gccgctgcgc
     3001 gaaacctcca attccttaca catgatg
//
LOCUS       DAOPPA010000408         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35544_length_3012_cov_3.728441, whole genome shotgun sequence.
ACCESSION   DAOPPA010000408 DAOPPA010000000
VERSION     DAOPPA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35544_length_3012_cov_3.728441"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKH54_11290"
     CDS             <1..475
                     /locus_tag="PKH54_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOD00521.1"
                     /translation="RVRAACPDVALSTDIIVGFPGETAADFESTMDAVRRCQFDLMFS
                     FKYSPRPGTAAASWKDDVPEAEKAARLAALQAMQDGITKSRMQRFLGRVEEVLVEGPS
                     RDSRKDGALQIMGRTRTNYIVNFTVPGGRVPSPGELVDVRIERILAHCLKGVVPG"
     gene            490..1362
                     /locus_tag="PKH54_11295"
     CDS             490..1362
                     /locus_tag="PKH54_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD00522.1"
                     /translation="MFFKAKDLMTVGNIGGGVAAIIVAMNGMSKATTPEEGKIWLFWS
                     AMCILFAWVFDAFDGKVARWLGQMNRFGSEFDNVADLVAYSVAPSFVVYFAYAKVLML
                     PGLASYPLAQQVIAVVLGAIPVTSGCIRFARFNTVRMDVPGFFIGFPRPASALLIVAL
                     LNSHLMEVSLTMRWVGVAMVVFLGIMNLTLMPFISHHGKEFSSHLKFVLHCVWMTVAF
                     SFLAGILVPLVSRLVGHPVKPLLPTNLVLDWVVIWLSFYVSVAWTEIPAETLRRVRIV
                     TAAYNRDSEHAPSH"
     gene            complement(1414..2031)
                     /gene="lexA"
                     /locus_tag="PKH54_11300"
     CDS             complement(1414..2031)
                     /gene="lexA"
                     /locus_tag="PKH54_11300"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOD00523.1"
                     /translation="MKPDKLTARQQEVLDFITSFTESSGAPPTVREIASAMGFASTNA
                     VSDHLAAIERAGFIERRSNRSRGIRVTAQARTSGAGQIPILGRIAAGRPIYAAENLEG
                     YLSPDPFFIRDTAETFVLCVTGRSMIGDGILDGDYIFVRRQPVADAGDIVVALIDDEA
                     TVKRYTRTPAGIALMPSNPDFKPIVVRPDDRFEILGVVTGVFRKM"
     gene            2205..>3012
                     /locus_tag="PKH54_11305"
     CDS             2205..>3012
                     /locus_tag="PKH54_11305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD00524.1"
                     /translation="MVSDDKNAAARGSDAASGGAAGSRDPTRQAGAAGRPTLVDEGNV
                     EARIEAAVSKIADDEQPARDPLIGRLIDNRFEVLSRLGAGGMGVVYRARQVGIDRIVA
                     IKTLLKDRAQDGKVVMRFKNEARAVSKLNHPNTIRIHDFGQMDDGTLYFAMELLEGRP
                     LDKAIRQDGPFSARRSIHVMTQMADSLAEAHSKGIVHRDLKPENVYLCAVGDDPDFVK
                     VLDFGVARMKEQDHSQGTMTQAGVIFGTPRYMAPEQARTMAVDARADIYAL"
BASE COUNT          567 a          856 c          951 g          638 t
ORIGIN      
        1 gagggttcgc gcggcctgtc ccgatgtcgc gctttcaacc gacatcatcg tcggttttcc
       61 cggtgagacc gccgcggatt ttgaatcgac gatggacgcc gtcaggagat gccagttcga
      121 tttgatgttt tcgttcaagt attcgccaag accgggaacc gcggcggctt cgtggaagga
      181 tgatgtgccg gaggccgaaa aggccgcgcg gctggcggcg cttcaggcca tgcaggacgg
      241 gattacgaaa tctaggatgc agcgtttcct tggtcgcgtc gaggaggtcc tggtcgaggg
      301 accgagcagg gattcaagga aggacggggc ccttcagata atgggccgca caaggacgaa
      361 ttacatagta aacttcaccg ttcccggcgg gcgggttccc agccccggag aactggttga
      421 cgtaaggatc gaaagaatcc tggcgcactg cctgaagggt gtcgtgccgg gttgaacagg
      481 gggaatcaga tgtttttcaa ggccaaggat ctgatgaccg tcggcaatat cggcggtggc
      541 gttgccgcga tcattgtggc gatgaacggg atgtcgaagg ccacgacacc cgaggaaggc
      601 aagatctggc tgttctggtc ggcgatgtgc atcctgtttg cgtgggtgtt cgacgccttc
      661 gacggcaagg ttgccaggtg gctgggtcag atgaacaggt tcggttcgga gttcgacaac
      721 gtcgccgacc ttgtcgcgta ttccgttgcc ccgtccttcg tggtctattt tgcgtatgcc
      781 aaggttctga tgcttcccgg cctggcatcc tatccacttg cccagcaggt aatcgccgtc
      841 gtgcttggcg ccattccggt cacttccggc tgcatcaggt tcgccaggtt caataccgtc
      901 aggatggacg ttcccgggtt cttcatcgga ttcccgcgcc ccgcctcggc cctgctgatc
      961 gtggccttgc tgaactctca cctgatggaa gtttccctta cgatgcgctg ggtcggggtc
     1021 gcgatggtcg tattccttgg tatcatgaac cttacactga tgccgttcat tagccatcac
     1081 ggaaaggaat tctcatcgca cctgaaattc gtgctgcact gcgtctggat gacggtcgcc
     1141 ttcagtttcc tggccggcat actggttccg ctggtttcgc gcctggtcgg ccacccggtc
     1201 aagccgctgc tgcccacgaa ccttgtgctt gactgggtcg tcatctggct gtcgttctat
     1261 gtttcggtag catggaccga aatcccggcc gaaacgcttc gccgcgtgcg catcgtcacc
     1321 gccgcctaca acagggattc cgaacacgct ccgagccact agccccattc ctttcaaatt
     1381 cgaatcggaa taatcgacgg cgaagctggc aggtcacatc ttccggaaaa caccggtgac
     1441 gacgcccaga atctcgaagc ggtcgtccgg cctgaccacg atgggcttga agtcggggtt
     1501 ggacggcatc agggcgatgc ccgccggcgt gcgcgtgtat cttttcacgg tggcctcgtc
     1561 gtcgatcagg gcaacaacga tgtcgccggc gtccgcgacc ggctggcggc ggacaaagat
     1621 gtagtcgccg tcaaggatgc catcgccgat catgcttcgc cccgtgacgc acaggacgaa
     1681 cgtttcagcg gtgtccctga tgaagaacgg gtcgggtgac agatatcctt caaggttttc
     1741 ggccgcgtag atcggccggc cggccgcgat ccgccccaga atcggaatct ggccggcccc
     1801 ggaagtcctt gcctgggccg tcaccctgat gccgcgggat ctgttcgaac ggcgttcgat
     1861 gaaaccggcc cgttcaatcg cggcaaggtg gtcggatacg gcgttcgtcg aagcgaatcc
     1921 catggccgac gcaatctccc tgacggtcgg gggcgcgccg ctggattcgg tgaagctggt
     1981 tatgaaatca agaacttcct gttgccttgc ggtcagtttg tccggtttca ttttggggga
     2041 ctctccatag gcgtgactat cctactgaac atttgttcac agtgcaacaa aacagcgcgc
     2101 gcgtcagggg gcgacggctt gacgggaact cggaacaatt ggcggtttgt gatagatttc
     2161 atccgtgcgc gttcgtcgtt ccagggacgt ttccggggat tgaagtggta tctgacgaca
     2221 agaatgccgc ggcgcggggt tccgatgccg catcgggcgg cgccgcaggt tcgcgggatc
     2281 cgacccgcca ggccggcgcc gccggaaggc cgacccttgt tgacgaaggc aatgtcgagg
     2341 cccgaatcga ggctgccgtc tcgaagatcg ccgatgacga acagcctgcg cgcgatcccc
     2401 tgataggccg tctcatcgac aacagattcg aggttctctc aagacttggc gcaggcggca
     2461 tgggcgtcgt gtatcgcgca aggcaggtcg ggattgaccg gattgtcgcg attaagacct
     2521 tgctgaagga cagggcgcag gacggcaagg tcgtcatgcg cttcaagaac gaggcccgcg
     2581 ccgtatcgaa gctgaatcat ccgaacacga tccgcatcca tgacttcggg caaatggatg
     2641 atggaacgct gtatttcgcg atggaactcc tggaaggccg cccgctcgac aaggcgatca
     2701 ggcaggatgg gcctttttcc gccaggcgtt ccattcacgt catgacacag atggccgatt
     2761 cccttgcgga agcgcattcc aaggggattg ttcatcgcga tctgaagccc gaaaacgtct
     2821 atctgtgcgc cgttggcgac gatcccgact tcgtgaaggt cctggatttc ggtgtcgcca
     2881 ggatgaagga gcaggatcac agccagggca cgatgacgca ggccggcgtc attttcggta
     2941 cccccaggta catggcgccg gaacaggcca ggacgatggc ggtcgatgcc agggcggaca
     3001 tctacgcgct cg
//
LOCUS       DAOPPA010000409         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35545_length_3012_cov_3.714576, whole genome shotgun sequence.
ACCESSION   DAOPPA010000409 DAOPPA010000000
VERSION     DAOPPA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35545_length_3012_cov_3.714576"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1621
                     /locus_tag="PKH54_11310"
     CDS             158..1621
                     /locus_tag="PKH54_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00525.1"
                     /translation="MIDGILKKVGLDLKIDRDAGPAVILARIPSWIDSRHTHPENCEA
                     PLDLGYCCTAASVRGFVPVLLDLETGVISSTDLIESVRRLCPAAVFLSGITPAVQAML
                     DIASKVRAAAPDCLVVAVGQHADYSPGTFLFPGSPFSLTTVGEFEETVGEVLAALRDG
                     HDPRVAGTRSIQGTSLVTHGERPATTDLDGLGMPDHRFFLSPVYRYLHPMRTPVRRRW
                     GFVQASRGCPFSCIYCSKTLRTSFGPTIRNRSPESVIDEMRWLNRHGITTVVFTDDLF
                     NSSKERVMHLCEAILSSGLKMGWTAEGRVKPTDLEMFKMMRRAGCSTFSMGVESGSPA
                     ILRTLDKKATVAEAERAFAQAREAGLLRVAFFMVGCPGETRYDFEMTRALLRRLKPEM
                     IQVANFTAYPGSRAYESYLKSMNLPWERFQHYERLTNLSAESDEMVRQWQKTLYMDLL
                     TGGDFAFRYVLKQNVGMLLNLDREISFAMKAARKLLQ"
     gene            complement(1563..>3012)
                     /locus_tag="PKH54_11315"
     CDS             complement(1563..>3012)
                     /locus_tag="PKH54_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOD00526.1"
                     /translation="SGHPQGVYFGLALGALYVIGEGWPRRETPWKSILAGGAGWLAVA
                     LAVASVQLLPLAANIQSTLHVFADPSRPEFDEDLIGYALTILGPAAVLVVLLPMRRRW
                     LEKGVFILSILFVLTITNTPIVRLWHAIVPMFEHFKLSSKYFVSPFPVLAAVALGLAV
                     GVASNWAGRSGRIALFMCGAMALWPVWSAPYEIVYRLQQPAGFVQAIDQECLKDIRDA
                     TPPMGRLLRFDTRAMYPSLTVTAQLFDVQVLATSLPARYAAVMSAITPENAQAIRQDS
                     NVGPVLDPAGLGLAFWDVAGVTTLISGSEVAAEGWIPVVSRPDSTCMTADRGYFIYRN
                     AQAFERAFLIQNDSAASARVEDLSFVIDPSTPPPRLAAGKVRIVDYEAARVRVEVDAP
                     STSRLVVSDAMADGWRVTVDGRPAAPATSWYLFRAVDLPAGKHTVVWEYRPSAAFAGL
                     VVGGAGLVAAFLWIIAAAFLPPSWRKRFRGQG"
BASE COUNT          620 a          935 c          930 g          527 t
ORIGIN      
        1 tcttcagggt atatttcaac ctgcggtaca tgacgcggta cgccgcgatg ataatcggtg
       61 gaatgaatcc gaccatgggc cgttaccttg tccaggaagc catcgcaaca ctgccggggg
      121 cccgcgggct gcctgccgaa caccaaaagg gcaacgaatg attgacggga ttctgaagaa
      181 ggtcgggctt gacctgaaga tcgacaggga cgccgggccg gccgtgatat tggccaggat
      241 accttcatgg atcgattcgc gacacacgca tccggaaaac tgcgaggccc cgctggatct
      301 tggctactgc tgcaccgccg catcggtgcg cgggttcgtt ccggttcttc tggatcttga
      361 aaccggcgtt atctcatcga ccgacctgat cgagtcagtc cgccggcttt gccccgcggc
      421 cgttttcctt tccggcatca cgcccgccgt ccaggcgatg ctggacatcg cttcgaaggt
      481 tcgggccgcg gcgcctgact gccttgtggt tgccgttggc cagcacgcgg actactcgcc
      541 tggcaccttc cttttcccgg ggtcgccgtt ctccttaacg accgtgggcg aattcgagga
      601 aaccgtcggc gaagtccttg cggccctccg cgacgggcat gacccgaggg tggccggcac
      661 caggtccatt cagggcacaa gtcttgtcac ccacggcgag cgcccggcga cgaccgacct
      721 cgacggactc ggcatgccgg atcaccgatt tttcctttcg ccggtctatc ggtacctgca
      781 cccgatgcgg acgccggtca ggcgcaggtg gggcttcgtc caggcttcac gcggctgtcc
      841 gttttcatgc atctactgtt ccaaaacgct tcggacatcg ttcggcccga caatccgcaa
      901 ccgcagcccg gaatcggtga ttgacgaaat gcgctggctg aatcgccacg gaatcaccac
      961 ggtcgtcttc acggacgatc ttttcaattc gtcgaaggaa cgggtgatgc atctgtgcga
     1021 agccatcctg tcgtccggcc tgaagatggg ttggaccgcg gaagggcgcg tcaagccgac
     1081 cgacctggag atgttcaaga tgatgcgccg ggccgggtgc tcgacgtttt cgatgggcgt
     1141 ggaaagcggc tcgccggcga ttctgcggac actcgacaag aaggcgaccg tcgccgaggc
     1201 ggaacgggct ttcgcgcagg cacgcgaagc cggccttctc cgcgtggcct tcttcatggt
     1261 cggatgcccg ggggaaacca ggtacgattt cgaaatgacg cgtgccctgc ttcgacggct
     1321 gaagccggaa atgatccagg ttgcgaattt tacggcctac cccggttcac gggcatacga
     1381 aagctacctg aaatcgatga acctgccgtg ggaacgcttc cagcattatg aaaggctgac
     1441 caatctcagc gccgaatcgg acgagatggt caggcagtgg cagaagacgc tttacatgga
     1501 tctgctgaca ggcggggatt tcgccttcag gtacgtcctt aagcagaacg tcggaatgct
     1561 tcttaacctt gaccgcgaaa tctctttcgc catgaaggcg gcaagaaagc tgctgcaata
     1621 atccaaagga aggctgccac aagtccggcg ccgccgacca caagaccggc aaaggccgcc
     1681 gacggcctgt attcccagac aaccgtgtgc tttcctgccg gcagatcgac agcccggaag
     1741 aggtaccacg aagtggcagg cgcggcgggc cgcccatcga ccgtgacgcg ccaaccgtcg
     1801 gccatcgcgt ccgaaaccac cagccttgat gtcgacgggg catcgacctc gacccgcacc
     1861 ctggctgctt cgtaatcgac aatccggact tttccggcgg ccaggcgagg cggcggggtt
     1921 gacggatcga tcacgaagga aaggtcttcg acgcgcgcgg acgcggctga gtcgttttga
     1981 atcaggaagg cccgttcaaa ggcctgggca ttgcggtaaa tgaagtatcc ccggtcagca
     2041 gtcatgcagg tcgaatccgg cctggacacc accgggatcc acccttcggc ggcgacttca
     2101 ctgcccgaaa tcagcgtggt cacgcccgcg acatcccaga acgccaggcc cagcccggcc
     2161 gggtcaagca cgggacccac gtttgaatcc tgacggatgg cctgggcatt ttcgggcgtg
     2221 atggcggaca tcaccgccgc gtacctggcc ggaaggctgg ttgcaagcac ctggacgtcg
     2281 aacagttgcg cggtgaccgt caacgaagga tacatcgcgc gcgtatcgaa ccgcagcaag
     2341 cggcccatcg gcggcgtggc atcgcggatg tccttcaggc actcctgatc gatggcctgg
     2401 acgaaccctg ccggttgctg caggcgatag acgatttcat acggcgccga ccagacaggc
     2461 caaagcgcca tcgcgccgca catgaaaagc gcgatcctgc cggatcggcc ggcccagttc
     2521 gacgcgacac cgaccgcaag gcccagcgcg acggcggcca acaccggaaa tggcgacacg
     2581 aaatacttcg aactcagctt gaaatgctcg aacatcggca cgatcgcatg ccacaggcgc
     2641 acgatcggcg tgttcgtgat ggtcagaacg aagaggatgg aaaggatgaa gacccccttt
     2701 tccagccatc tgcggcgcat cggcagaaga acgacaagga cggctgccgg tccaaggatg
     2761 gtcagggcat acccgatgag atcctcgtca aactcggggc gcgacggatc ggcgaagacg
     2821 tgaagggtcg attggatatt ggccgcaagc ggaagaagct ggacggacgc cacggccagc
     2881 gcgaccgcaa gccagccggc gccaccggcc agaattgact tccatggcgt ttcccggcgc
     2941 ggccatcctt cgccgattac ataaagcgcc ccaagggcaa gtccgaaata gacgccctgc
     3001 ggatgcccgg ac
//
LOCUS       DAOPPA010000410         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35593_length_3009_cov_3.434326, whole genome shotgun sequence.
ACCESSION   DAOPPA010000410 DAOPPA010000000
VERSION     DAOPPA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35593_length_3009_cov_3.434326"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKH54_11320"
     CDS             complement(<1..374)
                     /locus_tag="PKH54_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00527.1"
                     /translation="MMSFRKVARLVSATWMILAACSGQIACDDGSGVADALAIDYLPR
                     SVDFGSVVIGQQKTLSVTLRHSGESGVVVLGAISMEGLSGEFTTGQLSKTELAVGETA
                     TFDVIYRPTDASTDSGVIVVAH"
     gene            complement(566..2674)
                     /locus_tag="PKH54_11325"
     CDS             complement(566..2674)
                     /locus_tag="PKH54_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00528.1"
                     /translation="MNTKSCRGWRSVILAIVGLSLAMVGCEGGSANQFSSISISPGDR
                     AVNILVDSDYVVNITNTGEGRNLEITDVVLNYTPESADESEQGPAFILFKGTLPATLE
                     PVSSSPSSKTSFTLRYQAPKDGKGRSATVTVRNNNTTNFQDQELVISFDGKKCRPVLD
                     VESRVEFGFVSLGNDSYQDIGLVNTGTCDLVIDYIRVEGESVGHPEAFTVTVKGNDYV
                     GKSDFEQRMLPEVVTIAPGSGVMWGALFAPVDTNAAGATLVIHTNNTEVQDGLYFVDL
                     VANASGPKLVVDPNPIDFGGKLIQKTAGMMVSVRSTGDEPLVISQLALKEDTSADFKM
                     EIQGLAAAPTPENPVTLENGQAIDVMMTFTPNDENPRDNDGKPIPDTGTLLVGNNGIE
                     PNAEIPISGYGVTVDCPKPVIVIEEGELVKPQTLLHIHGEQSQSGSAGGSIIKYSWTV
                     DQPDENKFILLPSASSPTVTHEVNIGGEYTYCLDVCDNQYCSSDPKCGGSVCKTVKVI
                     PDKAIHCELTWHTPADTDEFDEGEDMGADMDLHFAHPFAAMTDIDKDGIRDPWFHVPY
                     DVFWFQPNPEWETPNPDGKDNPSLDRDDTDGAGPENLNLDSPVAGRVYRIGVHYWDDH
                     GFGASYPRLKCFIYGEEVFSVDMSVSGTAMYKCDMWYAAEIEWPQQTVTLLRNPDNSF
                     KITKGYEEPSFSAIGGGGCE"
BASE COUNT          644 a          874 c          862 g          629 t
ORIGIN      
        1 ttgtgcgcga cgacaatgac gccggagtcg gtcgatgcat cggttggcct gtaaatcacg
       61 tcaaaagtcg cagtctcgcc aacagcaagc tccgtcttcg aaagctggcc ggtcgtgaac
      121 tcgccggaaa gcccttccat cgaaatggcc ccgagcacga cgacacctga ttcgccggaa
      181 tgccgaaggg tgaccgacaa agtcttctgc tgtccgatga cgaccgatcc gaagtcaacc
      241 gaacgtggca aatagtcgat cgccaacgca tcggcgacgc cggaaccgtc atcgcaggcg
      301 atctggccgg aacacgcggc cagaatcatc cacgtggcgg aaaccaaccg ggcaactttt
      361 cggaatgaca tcatgatcga cccccgaacg aattgtttga aaaccatgcg aattacccga
      421 acggccaccg ggttacggtg tccggacaga acgctgcgcg actattttag taatctggaa
      481 gcaaattgaa acaataagtt gcgtgggaac cgacaatcca gttcaaactg ggcagcaaac
      541 aggggaaatg aaccggccgt caggactatt cgcaaccgcc gccgccgatg gcggagaagg
      601 acggctcctc gtaacccttt gtgatcttga acgagttgtc gggattgcgc agaagggtaa
      661 cggtctgctg cggccactcg atctcggccg cgtaccacat gtcgcacttg tacattgccg
      721 tgccggacac cgacatatcg acgctgaaca cttcctcgcc gtaaatgaag cacttaaggc
      781 gcggataact ggcgccgaag ccatggtcgt cccaatagtg aacgccgatt cgatagacgc
      841 ggcccgcgac cggcgaatca agattcaggt tttcagggcc ggcaccgtcc gtgtcgtcgc
      901 ggtcaaggga cggattgtcc ttgccgtccg gattcggggt ttcccattcg gggttcggct
      961 ggaaccagaa aacgtcgtag ggcacgtgaa accaggggtc acggattccg tccttgtcga
     1021 tatcggtcat ggccgcgaac ggatgggcga aatggagatc catgtccgcg cccatgtcct
     1081 cgccctcgtc gaactcgtcc gtgtcggccg gagtgtgcca ggtaagctcg cagtgaatcg
     1141 ccttgtccgg gatgaccttg acggtcttgc agacggaacc gccgcacttg ggatcgctgc
     1201 tgcagtactg gttgtcgcag acgtccaggc aataggtgta ctcgccaccg atgttgactt
     1261 cgtgcgtgac ggtcgggctg gacgccgacg gcagcaggat gaacttgttt tcgtccggct
     1321 gatccaccgt ccacgaatac ttgattatcg agccgccggc agaaccgctc tggctctgtt
     1381 cgccgtgaat gtgaagcagc gtctggggct tcacaagttc accttcctcg atcacgatca
     1441 ccggcttcgg acagtcaacc gtaacgccgt aaccggatat cggaatctcg gcgttcggct
     1501 caataccgtt gttgccgacc agcagcgttc ccgtatcggg aatcggcttt ccatcgttgt
     1561 cacgcggatt ctcatcgttc ggcgtgaagg tcatcatgac atcgatggcc tggccgtttt
     1621 cgagggtgac cgggttttcc ggtgtcggag ccgcggccaa tccctgaatc tccattttga
     1681 agtcggcact ggtgtcctcc ttcagcgcaa gctgcgagat caccaggggt tcgtcgccag
     1741 tggaacggac actgaccatc atgccagccg tcttctggat cagcttgccg ccaaaatcga
     1801 ttgggttcgg atcgacaacc agtttgggac cggacgcgtt ggcaaccaga tccacgaagt
     1861 aaaggccgtc ctgaacctcg gtgttgttcg tatggataac cagcgtcgca cccgcggcgt
     1921 tggtatcgac aggagcaaac agggcgcccc acatcacgcc gcttccgggg gcgatggtga
     1981 cgacctcggg cagcatgcgc tgctcgaagt cggactttcc gacataatca ttgcccttga
     2041 cggtgacggt gaacgcttcc ggatgaccga ccgactcgcc ttcaacgcga atgtaatcga
     2101 tgaccagatc gcacgttccc gtgttgacca ggccgatgtc ctggtatgaa tcgtttccga
     2161 gactgacgaa accaaactca acccgcgatt cgacgtccag gacgggacga cacttctttc
     2221 catcgaaact gattacaagt tcctgatcct ggaagttggt cgtattgttg ttgcggacgg
     2281 taacggtcgc ggaacgcccc ttcccatcct tgggagcctg gtaccgcaag gtgaacgacg
     2341 tctttgaaga cggacttgaa gacaccggct cgagggtcgc gggaagcgtt cccttaaaca
     2401 ggatgaaagc cggcccctgc tcgctttcat cggccgattc cggcgtgtag ttcaatacga
     2461 cgtcggtgat ttccaggttc cggccttcgc cggtgttcgt gatgttcacg acatagtcgg
     2521 aatcgaccag gatgttgaca gccctgtcgc cggggctgat tgaaatcgat gagaactggt
     2581 tggcggatcc accctcgcac ccgaccatgg caagtgaaag accgacgatc gccagaatga
     2641 ccgagcgcca accgcgacag gacttcgtgt tcataaacag cctctgcgtg ttaaaccggc
     2701 tgggaaaccg actgccggtc catttccgaa gataattcca gaggattatc cgaagcgccc
     2761 gatgcggtgt caagaaagga atgaccgcat tggaattgtt ttttcctttg actgtcccgg
     2821 ctttcggcaa catcgtctcg atgctgtgca attttgtctc tcttcctgcg ataatcacgc
     2881 catgaatcgg gcaaccacgg gtaactccac ggcagcatcc cagacgcatc gaccgcgcat
     2941 cgcggcagtg atgctggccg tgtgcgccgc ggccgtcatt ccatgggttg gaaccgtgac
     3001 gtacccgct
//
LOCUS       DAOPPA010000411         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35771_length_2998_cov_3.795787, whole genome shotgun sequence.
ACCESSION   DAOPPA010000411 DAOPPA010000000
VERSION     DAOPPA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35771_length_2998_cov_3.795787"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKH54_11330"
     CDS             complement(<1..530)
                     /locus_tag="PKH54_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD00529.1"
                     /translation="MEEKKKKRIGVYICHCGGNISDYVDVAQVRELAGKDDFVVVAKD
                     VMFACADSNQKDMVADIKEQGLDAIVVASCSPKLHLHTFRNVALRAGLNQYQYVQVNV
                     REQCSWPHHDDRKLATGKAAGLVEAGIRRVMGAESHETIRVSAARSVLIVGAGVAGMR
                     AALSLASMGNEVVLVE"
     gene            complement(534..>2998)
                     /locus_tag="PKH54_11335"
     CDS             complement(534..>2998)
                     /locus_tag="PKH54_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD00530.1"
                     /translation="KEPRFVIAENCTGCLQCEVVCPEVRSDAYNEDLADRKVAYIPFN
                     LANPRIASIERKGVSAPCIAGCPGGVKPYGYVSLVRAGQFEDAMDLHLEDVPLPGCLG
                     RACYAPCQAECTRASLEGPVDMRRIKRFFADDYYAKHLEPKPREKAAPTGRRVAIVGS
                     GPAGLTAAYALALRGHEVVIFEAAPLPGGMMRLTLPEFRLPNHVVDRDVKNVTALGVE
                     IRCNTRVTDLNSLKEQGFDAVFMATGTHDSTRLRAPGADLDGIVNCLDILRESKIGQK
                     RDLTGKKVMVVGGGNTAMDSARTAIRLGSDKVTVVYRRSLHEMPAFEMERHEALEEGV
                     EFAVLCNPVAFSGENGRVTSATLIRMELGEPGPDGRRQPVEVKGSEYKLPVDLVIEAI
                     GLSPGTGAFKDQLELRRDRTIAADERTLQTGVPWVFAGGDAVTGPTSIIEAVGQGRRA
                     AFYMDKWLAGQDLTAFEYGDTLPAVDKQTLLSRHDIPSAETLAEKFRDGRSRIVDFDD
                     IESTFTASEAVRSASRCMDCSNCRECHQCVVACPAYAIDFSQKKQVMNVKADAVVLST
                     GYDLWDPAEKPHYHSRQLPNVITSMQMERLIAPTRPFNAVLRPGDGKVPDNIAYVLCA
                     GSRDVSLDNYRTCPGASRNSQICSQICCMYSAKQAQLLMGALPLADITVYYMDIRAFG
                     KNYEEFFQQSKGMGISFVKGRVAKITDDGKGRGDVILRVEDVENGVVKEVKHDLVVLA
                     VGLRPQNLIVEKFLGQVPELDPYSYILQADPMVNPAVTTVDGVFVAGTAAAPMDIPDS
                     ILSAGAAAAQVAEYLNRKTVTD"
BASE COUNT          568 a          990 c          866 g          574 t
ORIGIN      
        1 ttctcgacca gcacgacctc gtttcccatg gacgccagcg atagcgcggc cctcatcccg
       61 gctacacccg ccccgacaat caggacggat ctggcggccg acacgcggat cgtctcgtgg
      121 gactcggcgc ccatgacccg ccggatgcct gcctcgacca gtccggcggc cttcccggtc
      181 gccagcttcc tgtcgtcgtg atgtggccag gaacactgct cgcgcacgtt gacctggacg
      241 tactgatact ggttcaggcc ggcccgcaaa gccacgttcc gaaaggtgtg cagatgcaat
      301 ttcggggaac agcttgcgac aactatcgcg tcaagcccct gctccttgat gtcggccacc
      361 atgtccttct ggttgctgtc ggcacaggcg aacatcacgt ccttggccac gacgacgaaa
      421 tcgtccttgc cggccagttc gcggacctgg gccacatcga cgtagtccga gatgttgccg
      481 ccgcagtgac agatatagac gccgatacgt ttcttctttt tttcttccat acgtcagtcc
      541 gtcaccgtct tcctgttcag atattcggca acctgggcgg ccgccgcgcc agcggaaaga
      601 atcgagtccg ggatgtccat cggcgccgcc gccgtgcccg ccacgaacac gccatcgaca
      661 gttgtcacgg ccggattcac catgggatcc gcctgcagga tgtagctgta gggatccagc
      721 tccggaacct ggcccaggaa cttttcaaca atcaggttct gcggacgaag cccgaccgcc
      781 agcacgacca ggtcgtgctt cacttccttg accacgccgt tttccacgtc ctcgacacgc
      841 aggatgacgt cgccgcgacc ctttccgtcg tcggtaatct tcgcgacgcg ccccttgacg
      901 aagctgatgc ccatgccctt gctctgctgg aaaaactcct cgtaattctt gccgaaagca
      961 cggatatcca tgtaatagac cgtgatatct gccagcggaa gcgcacccat aagaagttgg
     1021 gcctgcttgg cggagtacat gcagcagatc tgcgagcaaa tctggctgtt acgcgatgcg
     1081 cccgggcagg tccgataatt gtccaggctg acgtcgcgag acccggcaca aaggacgtac
     1141 gcaatattgt cgggaacctt gccgtcgccc ggccggagca ccgcgttgaa cggacgggtc
     1201 ggagcgatca ggcgttccat ctgcatggaa gtgatcacgt tcggaagctg ccgggaatgg
     1261 taatgcggct tctcggccgg atcccacaga tcatatcctg tcgaaagcac gacagcgtcc
     1321 gccttgacgt tcatcacctg cttcttctgg ctgaagtcga tcgcgtatgc cggacaggcg
     1381 acaacgcact ggtgacactc gcggcagtta ctgcagtcca tgcagcgcga agcagaccga
     1441 accgcttccg aagccgtgaa ggtgctttcg atgtcgtcga aatcgacaat cctggatcga
     1501 ccgtcgcgga acttttccgc aagtgtttcg gccgaaggga tatcgtgccg ggacaacaga
     1561 gtctgcttat cgacggccgg cagcgtgtcg ccatactcga acgccgtcag atcctggccg
     1621 gccagccact tgtccatgta aaacgcggcc ctgcgaccct gccctacggc ctcgatgatc
     1681 gaggtcggac cggtcaccgc atccccgccg gcgaaaaccc acgggacgcc ggtctgaagc
     1741 gtcctttcgt ccgccgcgat ggtccgatcc ctgcgcagtt caagttgatc cttgaaggcg
     1801 ccggtgccgg gactcaggcc gatcgcctcg atgaccagat caaccggcag tttgtattcc
     1861 gaacccttga cctcgacagg ctgcctgcgg ccgtcgggac caggttcgcc aagctccatc
     1921 ctgataagcg ttgctgacgt tacgcggccg ttttcgcccg aaaaggcaac ggggttacac
     1981 aggaccgcaa actcgacgcc ctcttccagg gcctcgtggc gctccatctc aaacgccggc
     2041 atctcgtgca gactgcggcg atagacgacc gtcaccttat cggagcccag cctgatggcg
     2101 gtcctggccg agtccatcgc ggtattccca cccccgacca ccatgacctt ctttccggtc
     2161 aggtcacgct tctggccgat tttcgattcc cgcaggatgt ccaggcagtt gacgattccg
     2221 tccagatcgg cccccggggc gcgcaatctg gtggagtcgt gcgtccccgt cgccatgaac
     2281 acggcgtcga acccctgttc cttcagcgaa ttcaggtcgg tcacgcgggt attgcagcga
     2341 atctcgacgc ccagggccgt cacgttcttc acatcgcgat ccacgacatg gttgggaagc
     2401 ctgaactcgg gcagggtcag gcgcatcatg ccgccgggaa gcggggcggc ttcgaaaatg
     2461 acgacttcat gcccccgcag ggccagcgcg tacgcggcgg tcaggccggc ggggccggaa
     2521 ccgacgatcg cgacgcgcct gccggtgggc gccgccttct ccctgggctt gggctccagg
     2581 tgcttcgcat agtagtcatc agcgaaaaat cgcttgatgc gccgcatatc gacagggccc
     2641 tcgaggctgg cccgggtaca ctctgcctgg cacggcgcgt agcacgcacg accaagacag
     2701 cccggcagag ggacgtcctc cagatgaaga tccatggcgt cctcgaactg cccggcacgc
     2761 acaagcgaca cataaccgta aggcttgacg ccgcccggac atccggcgat gcacggcgcc
     2821 gaaacgccct ttcgctcgat tgacgcaatc ctgggattcg ccaggttgaa cgggatatag
     2881 gcgaccttgc ggtcggccag atcctcgtta tatgcgtcac tgcgaacctc ggggcatacg
     2941 acttcgcact gcaggcaacc ggtgcaattc tcggcgatga cgaaccttgg ctccttcg
//
LOCUS       DAOPPA010000412         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35847_length_2992_cov_4.042901, whole genome shotgun sequence.
ACCESSION   DAOPPA010000412 DAOPPA010000000
VERSION     DAOPPA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35847_length_2992_cov_4.042901"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKH54_11340"
     CDS             complement(<1..897)
                     /locus_tag="PKH54_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD00531.1"
                     /translation="MSFDEIDQKWVQGSLAKTLAKAPERQPAFTTPSGSEIRRLYTPA
                     DSRVGTDPVQYSERIGFPGTFPFTRGVQPTMYRGRLWTMRQYAGFGTAKESNRRYRYL
                     LEQGQTGLSVAFDLPTQIGMDSDSPMAAGEVGKVGVAIDTLADMEILFDEIPLDRVTT
                     SMTINAPAMVLLAMYIAVAGKRGIPASELGGTIQNDILKEYIARGTYIFPPRPSMRLI
                     TDIFGYCTGNVPKWNTISISGYHLREAGCTAVQEVAFTLADGIAYVEAAIKAGLDVDD
                     FASRLSFFFNVHNNFIEEVAKFR"
     gene            complement(1076..>2992)
                     /locus_tag="PKH54_11345"
     CDS             complement(1076..>2992)
                     /locus_tag="PKH54_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOD00532.1"
                     /translation="ELTFTNKGPDEGTVTATLVIHSNSKGLERLEVPLQAKRSGAPYC
                     RAQLVPGTVNFGVIAIGFPETKEARLVNTGTGDCTVTGFKLADCTSGMMGSSCPQPLT
                     GSNSQFFKVVGESPMSAKIGGGSFRSIIIEFTPPTVTDIFNLLSQSSAIMSFKVKDDF
                     NNQESVIPECSGTCNYNVVGQSGIAKAAVLPDHIDFGVVTIGCFSKTYSVCVYNSGNA
                     PLKIKDLKFKGCTPEFHFKKVPALPKVVGTGAPVCFEANYSPLDEGVDQCAIHVSVTD
                     KSAPIIAIPLKGEGTYETQHTDEFIQIKGDEVDILFVIDDSGSMCEEQSRLSASFGKF
                     IAQASVWDNDYHIGVISVNVVDEPVIGRLNRGDKTVTPRYITKSGNAQSQFAKLVNIG
                     CDGGSDAQEAGLQAAQTALAAPLTTDTQVTCGSNTDCTNDANICADPANCPYTCIDGS
                     CGGFNKGFMREDAQLEIIILSDEEDQSSSSISFYVDFLTNIKGWANQGRMHVNAIVGV
                     KGVPESSSEGACAAADGGTAAEGKRYIDACDMTGGQVGSICESSFAPIMEDIADITFN
                     PKLQFFLSRLADPATVKVSVDHQGDGNFVPMSDCWEFDAPSNSVIFSNEPPCVPPQPQ
                     DHIKVSYKMLCLKG"
BASE COUNT          565 a          855 c          933 g          639 t
ORIGIN      
        1 cctgaacttg gcgacttcct cgatgaagtt gttgtgaacg ttgaagaaga acgacagtct
       61 gctggcgaag tcatccacgt ccagaccggc cttgatggcg gcctcgacgt aggcgatacc
      121 gtccgcgagc gtgaaggcga cctcctggac ggcagtgcat ccggcctctc gcaggtggta
      181 ccccgaaatc gaaatcgtgt tccacttcgg cacgttgccg gtgcagtaac cgaagatgtc
      241 ggtaatcagg cgcatcgaag gtcgcggcgg gaatatatag gtgccgcgcg cgatgtactc
      301 cttcagaatg tcgttctgga tcgtgccgcc cagttcggac gccgggattc cccgctttcc
      361 ggcaaccgcg atgtacatgg ccagaagaac cattgccgga gcgttgatcg tcatcgatgt
      421 agtgacgcga tcaagcggaa tttcgtcaaa caggatctcc atgtcggcaa gcgtgtcgat
      481 cgcgacaccg accttgccga cctcgcctgc cgccatcgga ctatcggaat ccatgccaat
      541 ctgggtggga aggtcgaagg ccaccgacag cccggtctgg ccctgttcga gaagatagcg
      601 gtacctgcgg ttcgactcct ttgcggttcc gaagcctgcg tactggcgca tcgtccacag
      661 acgcccacgg tacatcgtcg gctgcactcc ccgggtgaag gggaaagtgc ccgggaagcc
      721 gatccgttcc gaatattgaa ccgggtccgt gcccaccctg gaatctgccg gggtgtaaag
      781 gcggcggatc tcggaacctg acggcgtcgt gaaggccggt tgcctttccg gtgccttcgc
      841 gagtgttttt gccaatgaac cctgaaccca cttctggtct atctcatcaa acgacacttg
      901 aatgctccgc aaatcctttg tcacgggcac gtccgggcgt ccgggaagcc cggtcggatt
      961 ctaacccctt cgcgtcgatt ttgcatcctg cgtgcggacg taatttcccg aaatcgaaca
     1021 acttgatgct ttcaggatgg cacttgttct gccgggtgga tgttacgatg aggtgctaac
     1081 ccttcaggca gagcatcttg taggacacct tgatgtgatc ctggggctgc ggcgggacgc
     1141 acggcggttc gttgctgaag ataacggagt tcgacggggc gtcgaattcc cagcagtccg
     1201 acatcgggac gaagttgccg tcgccctggt ggtcaaccga caccttgacg gtcgccgggt
     1261 cggccaggcg ggaaaggaag aactgcaact tcgggttgaa ggtgatgtcg gcgatgtctt
     1321 ccataatcgg ggcaaaagac gactcgcaga tcgagccgac ctgtccgccg gtcatgtcgc
     1381 acgcatcgat gtaacgcttg ccctcggccg ccgttccgcc gtctgcagcg gcgcacgcac
     1441 cctcgctgct tgattccgga acgcccttga cgccgacgat ggcgttgacg tgcattctgc
     1501 cctggttggc ccatcccttg atgttggtaa ggaagtcaac atagaaggaa atggacgacg
     1561 aggactgatc ttcctcgtcg gagaggatga tgatttcgag ctgggcgtcc tcgcgcatga
     1621 agcccttgtt gaaaccgccg caggaaccgt cgatgcaggt gtacgggcag ttcgccggat
     1681 cggcgcagat attggcgtcg ttcgtgcagt ccgtgttgct gccgcatgtg acctgggtat
     1741 cggtggtcag tggtgccgca agcgccgtct gggccgcctg caggccggcc tcctgggcgt
     1801 cggaaccgcc gtcgcagccg atgttgacca gcttggcgaa ctggctctgg gcgttcccgg
     1861 acttcgtgat gtaacggggc gtgacagtct tgtcgccgcg gttgagcctg ccgatgacag
     1921 gttcatcgac gacgttaacg ctgatgacgc cgatgtgata atcgttgtcc cagaccgacg
     1981 cctgggcgat gaacttgccg aaggacgcgg acaggcggct ctgctcctcg cacatcgaac
     2041 cggagtcgtc gatgacgaaa agaatatcga cctcgtcacc cttgatctgg atgaattcgt
     2101 cggtgtgctg ggtctcgtat gtgccttcac ccttcagggg gatcgcgatg atcggcgcgc
     2161 tcttgtcggt gaccgacacg tgaatcgcgc actggtcaac accctcgtcc agcggcgagt
     2221 agttggcctc gaaacagacc ggggcgccgg ttccgacgac cttcggaagc gccggaacct
     2281 tcttgaaatg gaattccgga gtgcagccct tgaacttcag atccttgatc ttcaaaggtg
     2341 cgttgcccga gttatagacg caaacgctgt aagtcttcga gaagcagccg atggtcacga
     2401 cgccgaagtc gatgtggtcg ggaagcacgg cggccttcgc gatgccgctc tgccccacaa
     2461 cgttgtagtt gcaggtgccc gagcattcag ggatgaccga ttcctggttg ttgaagtcgt
     2521 ccttgacctt gaacgacatg attgccgaac tctggctgag caggttgaag atgtcggtca
     2581 cggtcggcgg cgtgaactcg atgatgatgg atctgaagct gccgccgcca atcttggcgc
     2641 tcatcggcga ttcgcccacg accttgaaga actgcgagtt gctgccggta agcggctgcg
     2701 ggcagcttga acccatcatg ccgctggtgc agtcggcaag cttgaagccc gtcaccgtgc
     2761 agtcgccggt tccggtgttg accagtcggg cttccttcgt ttccgggaat ccgattgcga
     2821 tgacgccgaa attgacggtg ccggggacaa gctgcgcacg gcagtaaggg gcgccggaac
     2881 gcttggcctg aaggggaact tcaagtcttt cgagaccctt cgagttcgag tggattacca
     2941 gggtcgcggt gaccgtgcct tcatccggac ccttgttcgt gaaggtcagt tc
//
LOCUS       DAOPPA010000413         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35849_length_2992_cov_3.943139, whole genome shotgun sequence.
ACCESSION   DAOPPA010000413 DAOPPA010000000
VERSION     DAOPPA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35849_length_2992_cov_3.943139"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKH54_11350"
     CDS             <1..515
                     /locus_tag="PKH54_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOD00533.1"
                     /translation="FPVNCVNRAMAADYATFVGGRLPTEAEWEYAAGLGEGTVKGRQY
                     VLAVEDYSEPGKPKNPDYSEINDYAWYRINSGGKCHPVATRKPSSVGLYDMLGNVMEW
                     VSDWMGPYTAAPVTSPTGPLDGSMAQVRGGGWNSHGFEARIHDRQYVEADATGIYLGF
                     RPVKDLPTRK"
     gene            complement(638..1429)
                     /locus_tag="PKH54_11355"
     CDS             complement(638..1429)
                     /locus_tag="PKH54_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD00534.1"
                     /translation="MLRVLKSRLLQKHRTFKYPNGPAPHLSDRFLGRPVLSGRCPDGC
                     RVCADSCPFQAIAIRSRDESIGNSATMPVELDMGRCIFCGACARACPEGNIVFSNNWR
                     LAAARREDLVITSESPTEANCRSDVVCRYYRHSLKLRQVSAGGCNACEADSNVLGTPA
                     WDMGRFGISFVASPRHADALLVTGPVSRNMRLALEKTFAALPGPRVVIACGTCAISGG
                     PFAGHPEVESGAESTVPIDLFIPGCPPHPLTILDALLRLQGIKTP"
     gene            complement(1439..2950)
                     /locus_tag="PKH54_11360"
     CDS             complement(1439..2950)
                     /locus_tag="PKH54_11360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOD00535.1"
                     /translation="MLMLKNSQAVPFADIPHTTMGEWLRIVMDATQAGYGLSALFAVK
                     DGERHQVFTVLSSREQHRIGVVRTTIGQAYPALTTDFPAAHWFERELYEQYGIEPEGH
                     PWLKPIRFEPSVIPGRYVPDIGMAPFFEMEGQEVHEVGVGPVHAGVIEPGHFRFQCHG
                     ETVHHLEISLGYQHRGIERALVGGPGPRTIHLVETAAGDATVGHTDPFCRAIEALAGV
                     EPGLHARTVRAIGTELERLACHCGDLGALAGDVGFLPTQNYCGRIRGDILNLTALICG
                     NRFGRGLAVPCGTRHSLEPGRCLLLLNRLDDIKRDLEDAIPLMFETPSVMNRMEGTGI
                     VTPETAKAIGLVGPAARASGLPYDVRWDLDWEPWSHLDFKVPTCQSGDCAARARIRWA
                     EIQVSIEIVNKLVSGILEGDDTGAAADCAINGDSLPDLCPDHFVVSMGEGWRGEICHV
                     AATDASGRFAFYKIVDPSFHNWFGLAMALRNQQISDFPLCNKSFNLSYCGHDL"
BASE COUNT          601 a          890 c          922 g          579 t
ORIGIN      
        1 atttcccggt gaactgcgtc aaccgggcga tggcggccga ttatgccact ttcgtgggcg
       61 gccgcctgcc gaccgaggcg gaatgggagt acgcggctgg tcttggcgaa ggcacggtca
      121 agggaagaca gtacgtcctg gccgttgaag actattccga gccgggaaag ccgaagaatc
      181 cggactactc ggaaatcaac gattacgcgt ggtatcggat taacagcggc ggcaaatgcc
      241 atcctgttgc gacaaggaag ccgagttcgg tcgggcttta tgacatgctt ggaaacgtga
      301 tggaatgggt ttccgactgg atgggcccgt acacggccgc tccggtcacc agtcccaccg
      361 ggccgctcga cggatcgatg gcccaggttc gtggcggtgg atggaacagt cacgggttcg
      421 aggccaggat ccacgatcgt cagtatgtcg aggccgatgc aaccggcata taccttggct
      481 tccgcccagt caaagacctt cccacacgca agtaattccg atccgtacgg gcgaatgaaa
      541 attcgcccgc ctccgatgaa aatccgcccg ctgctctcct tgtacgggcg gattacatcc
      601 gcccgctgcg gtcaagcaca ttgccttcaa tctatctcta cggtgtcttg attccctgca
      661 ggcgcagcag tgcatccagg attgtcaggg ggtggggcgg gcagccgggg atgaaaaggt
      721 cgatcggaac ggttgattcg gccccgcttt caacttccgg gtgcccggcg aacggcccgc
      781 cggaaatcgc gcaggtcccg caagcaatga cgaccctcgg gcccggcagt gcggcaaaag
      841 tcttttcaag cgccagtctc atgttgcggc tgaccgggcc ggtaacaagc agagcgtcgg
      901 cgtgtcttgg cgaagcaacg aaggaaattc cgaacctgcc catgtcccat gccggcgttc
      961 caagaacgtt cgaatcggct tcacatgcgt tgcagccgcc ggccgacacc tggcgcagct
     1021 tgagcgagtg tcggtagtaa cggcagacga catcggaacg gcaattggcc tcggtcggcg
     1081 attcggatgt aataaccagg tcttcccgtc ttgccgccgc caggcgccag ttgttcgaga
     1141 agactatgtt gccttcgggg caggcccttg cgcacgcgcc gcagaaaatg catctgccca
     1201 tgtccagttc gaccggcatc gtggcggaat tgccgatgga ttcgtcacgc gaccggattg
     1261 cgatggcctg gaaagggcaa gaatcggcgc agactcggca accgtccggg cagcggcctg
     1321 aaagtaccgg ccgtcccaga aaacggtcag aaagatgcgg ggccggtccg ttcgggtact
     1381 tgaacgtccg gtgtttctgc agaagcctgg atttcaggac tcgaagcatt ggttcaccct
     1441 aaaggtcatg cccgcagtag gacaggttga aactcttgtt gcacagcgga aagtccgaga
     1501 tctgttgatt gcgcagggcc atggccagtc cgaaccagtt gtggaaggac ggatcgacta
     1561 tcttgtagaa cgcgaagcgt ccggaagcat cggttgccgc cacatggcag atttcaccac
     1621 gccatccttc ccccatggat acgacgaagt ggtcggggca aaggtcgggc agactgtccc
     1681 cgttgatggc gcagtcggcg gccgccccgg tgtcatcacc ttcaagaatg ccgctgacca
     1741 gcttgttcac gatctcaatc gaaacctgta tctcggccca tctgatgcgg gccctggccg
     1801 cgcagtcgcc cgactggcag gttggaacct tgaagtccag atggctccac ggctcccagt
     1861 caagatccca cctgacgtcg tacggcaggc cgctggccct ggcagccggg ccgaccagtc
     1921 cgatcgcctt tgccgtttca ggggttacga tgccggtacc ctccatcctg ttcatgacgg
     1981 aaggtgtttc aaacatcagg ggaattgcat cttcaagatc gcgtttgata tcgtcaagac
     2041 ggttcagcag aagcaggcag cggcccggtt caagtgaatg ccgggttcca catggaactg
     2101 caaggccacg tccaaagcgg tttccgcaaa tcagcgccgt caggttcaag atgtcgcctc
     2161 tgatgcgtcc gcagtagttt tgcgtgggca ggaatcccac atcgcccgcc agggcgccaa
     2221 ggtcgccgca gtggcacgcc agccgttcaa gttcggttcc gattgcccgt accgtccgcg
     2281 cgtgcaggcc aggttcgaca ccggcgaggg cctcgattgc acggcagaac gggtcggtgt
     2341 gtccgaccgt ggcatcgccc gccgccgtct cgacaaggtg gatggtgcgt ggtcccggtc
     2401 cgccaaccag ggcgcgttca attccacgat gctggtaacc aagcgagatc tccaggtgat
     2461 gaaccgtttc gccgtgacac tggaaacgga agtgcccagg ttcgataacg ccggcgtgta
     2521 ccgggcccac accgacctcg tgaacttcct ggccttccat ttcgaagaac ggcgccatgc
     2581 cgatgtccgg cacgtaacgg ccaggaatca cggatggttc aaatcggatt ggcttcagcc
     2641 agggatggcc ttccggttca atgccgtatt gttcatataa ctcacgctcg aaccagtggg
     2701 cggcggggaa gtcggttgta agggccgggt acgcctggcc gatggtggtc ctgacgacac
     2761 cgatcctgtg ctgctcccgg gacgacagaa ccgtgaagac ctggtgacgc tcgccgtcct
     2821 tcacagcaaa gagtgccgaa agaccgtatc cggcctgggt cgcgtccatc acgattcgaa
     2881 gccattcgcc cattgtcgta tgggggatgt cggcgaaagg cacggcctga ctgttcttca
     2941 gcatcagcat ggctattgcg cgcccccgtt tccaccaatg actgccgcgg cg
//
LOCUS       DAOPPA010000414         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_35954_length_2985_cov_3.796246, whole genome shotgun sequence.
ACCESSION   DAOPPA010000414 DAOPPA010000000
VERSION     DAOPPA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35954_length_2985_cov_3.796246"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PKH54_11365"
     CDS             complement(<1..1266)
                     /locus_tag="PKH54_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00536.1"
                     /translation="MIVGLIIALVGALGGLIGSLVLPWVDTDAVKFADPSAYAGYAWP
                     TGVVAIILLGAAVVLVGLGFAMERKRKLFAIINMFVALGVLVFSFLTPFSIRTAINEM
                     ILAGTIPEATRLAMFYGYHAIFFGNVVLAFGCAFLLGQAGSYRTDDKLLRVAHLWHGR
                     IIRETTITEHHDIKIGGAAGCNFIVPLTADPESNDSITLFKFLDRSRFGLVLNKDMHG
                     RVNINHKLKSVKDAVAQDADSNGIVNLAMGDWGTIDFGFNQIFFNYVHPDVVIGRGGA
                     SKTGEGPFVAAMAASAFVAVALWIGSQFAWDPAGSIERHASQRKIMKVEAAVSMEKDE
                     ILLDLGEEEEDDVGKRAEGEEGKFGDPDNNPDLENKVPKRDGQMAAKIDVKNVGLADL
                     LSNKMGKGGAISSIPSDNTDAFENRMAVAM"
     gene            complement(1461..2216)
                     /locus_tag="PKH54_11370"
     CDS             complement(1461..2216)
                     /locus_tag="PKH54_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00537.1"
                     /translation="MTRRRLAAACLTAAILVAAQFPQISPVVAQEAGGATGQAAFTPQ
                     QAAAILSAGDKAFRTGRVGDALRTFTTIFTVFPTWWIAGAKVAVASAQSGQTPDEIVA
                     ALKALVPMGPTGPWVPMLAVMAAASRLAQEDVEFIKHMSRQMLAEPPEDEAYLMRTRD
                     ADLRSRYALARGLALEAAGQPGMAVAEYRALSESRPGAIAPRVRLAVLLSAVGRHADA
                     DTILAPIEPVSLMPSRIRMLRQQIRATISPDPR"
     gene            complement(2213..>2985)
                     /locus_tag="PKH54_11375"
     CDS             complement(2213..>2985)
                     /locus_tag="PKH54_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00538.1"
                     /translation="NELGVPESIGRPRVRVSASVPSDQFAAIPSDVSASISARSVLGE
                     KHLELTPGNGAVPPLKAGTVLQGRPPAGIEKAGDDAVSLIRRLERFMDENEGKLSGAI
                     VQVNDLATGLNAFLKVNGPKLDEAVENLRLASAGLAREAGPGGRVSSLMITADRVVKR
                     IDAATAGTEKSVPELIADVSRLSARADHLLAGMDRLVTDMTGPTVGAVRNLNDISETI
                     SQGKGTIGKLVLDQTLYDDVSAFVKEIKAKPWKLLWKK"
BASE COUNT          619 a          904 c          886 g          576 t
ORIGIN      
        1 catcgcgacg gccatgcggt tttcaaacgc gtcggtgttg tcggaaggga tggaggaaat
       61 cgcgccgccc ttgcccatct tgttcgaaag gagatccgcc aggccgacgt tcttgacgtc
      121 gatcttcgcg gccatctggc catcgcgctt cggaaccttg ttctccaggt cggggttgtt
      181 gtccggatcg ccgaacttgc cttcctcgcc ctcggcgcgc ttgccgacgt cgtcctcttc
      241 ctcttcgccg agatcaagaa ggatttcgtc cttttccatc gaaacggcgg cctcgacctt
      301 catgatcttg cgctggctgg cgtgcctttc gatgctgccc gccggatccc aggcgaactg
      361 gctgccgatc cacagggcga ccgcgacaaa ggcgctggcc gccatcgcgg caacgaacgg
      421 gccttcgccg gtcttggaag cgccgccacg tccgattacg acgtcgggat gaacatagtt
      481 aaagaagatc tggttgaaac cgaaatcaat agtgccccag tcgcccatcg cgagattgac
      541 gatgccattc gaatcggcat cctgggcaac ggcgtccttc actgacttca gcttgtgatt
      601 gatattgacg cggccatgca tgtccttgtt cagaaccagt ccgaaccgac tgcggtcaag
      661 gaacttgaaa agcgtgatgg aatcgttcga ttcaggatcc gcggtcagcg gaacgatgaa
      721 attgcaaccg gcggcgccac cgatcttgat gtcgtgatgt tcggtgatcg tggtttcccg
      781 gatgatgcga ccgtgccaca gatgcgccac gcgaagaagc ttgtcgtcgg tcctgtagga
      841 gccggcctgt ccaagcagga acgcgcaacc gaatgccagc acgacgttgc cgaagaatat
      901 ggcgtgatag ccatagaaca tcgcaagccg ggtggcctcg ggaatggtgc ccgcaaggat
      961 catttcgttg atggccgtgc ggatcgagaa cggggtcagg aaggagaaca cgagaacgcc
     1021 gagcgccacg aacatattaa tgatggcgaa cagcttcctc ttgcgctcca tggcgaagcc
     1081 gagtccgaca agcacgaccg ccgcgcccag aagaattatc gcgaccacac cagttggcca
     1141 cgcgtaaccg gcgtaggccg atggatcagc gaacttgacg gcgtccgtat cgacccaggg
     1201 cagaaccagt gagccgatga gaccgccgag cgctccgacc agtgcgataa tgagtccaac
     1261 tatcatgaca ctgtccccgc aaacgtgttc cgattcatac gatttccagc aaatccgcga
     1321 tcttaagcgg tgccggacaa tcgcagcctg ttcaacggca atgcatgaac attcaggccg
     1381 tccgtataat gcaaagacct tggaataagg tcaatcaaaa aaaatcgaaa cggacaaaaa
     1441 ttcggcagaa aagatcacat tcagcgcgga tccggggaga ttgtggcccg gatctgctgc
     1501 cgaagcatgc ggattcgaga tggcataagg ctgacaggct cgattggcgc cagtatcgtg
     1561 tcggcgtcgg catgcctccc gaccgctgaa agaaggactg caagacggac tctcggcgca
     1621 atcgcgccag gccgtgattc ggaaagggca cggtactcgg cgacggccat tccaggctgg
     1681 ccggcggcct cgagagccag tccccgtgcg agtgcatagc gactgcgcag gtcggcgtcc
     1741 ctggttcgca tcagatatgc ctcgtcttcg ggaggctcgg ccagcatctg acgagacatg
     1801 tgcttgatga attcaacatc ttcctgtgca agcctggatg cggcggccat gacggcaagc
     1861 atcggcaccc acggcccggt aggtcccatt ggcacaagag ctttcaacgc ggcaacgatt
     1921 tcgtcggggg tctggccgga ctgcgcggat gccacggcga ccttggctcc ggcgatccac
     1981 caggtgggaa acaccgtgaa tatggtcgtg aatgtcctta gcgcatctcc cacccggccg
     2041 gttcgaaatg ccttgtcgcc cgcggacagg atagccgcgg cctgctgtgg ggtaaacgca
     2101 gcctggccgg tggcaccgcc agcttcctgg gccacgacgg gggaaatctg cggaaactgc
     2161 gcggcgacaa ggattgccgc cgtcaggcat gcggccgcaa gtcgccgacg cgtcatttct
     2221 tccacagcag cttccatggc ttggccttaa tttcctttac gaacgccgaa acgtcgtcat
     2281 acaacgtctg atccagaaca agctttccga tggttccctt gccctggctt atcgtctcgc
     2341 tgatgtcgtt gaggttcctg acagcgccga ctgtcggtcc ggtcatgtct gtgacgagcc
     2401 gatccatccc tgcaagaaga tgatccgccc tggccgagag ccttgaaacg tccgcgatga
     2461 gttccggaac cgacttctcg gtgccggcgg ttgcagcatc gatccgcttc acaacgcggt
     2521 cggctgttat catcaggctg gagacccgcc caccgggacc tgcctcccgg gcaagtcccg
     2581 cagaagcaag gcgcaggttc tcgacagcct catcgagctt cgggccgttg accttgagaa
     2641 aggcgttcaa gcccgtagcg aggtcgttaa cctggacgat tgcgcccgaa agctttccct
     2701 cgttttcgtc catgaacctc tcaaggcgac gaatcagcga aaccgcgtcg tccccggcct
     2761 tctcgatgcc ggcgggcgga cgtccctgca gaaccgttcc ggccttcagc ggcggcacgg
     2821 cgccgttgcc cggcgtcagt tccagatgct tctcgccaag cacgctgcgg gccgaaatgg
     2881 atgcggaaac gtcagatgga atagccgcga attgatccga cggaaccgat gccgaaacgc
     2941 gaaccctggg gcgcccgatg ctttcgggca cgccgagttc attgc
//
LOCUS       DAOPPA010000415         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_36086_length_2977_cov_3.413758, whole genome shotgun sequence.
ACCESSION   DAOPPA010000415 DAOPPA010000000
VERSION     DAOPPA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36086_length_2977_cov_3.413758"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..2322
                     /locus_tag="PKH54_11380"
     CDS             220..2322
                     /locus_tag="PKH54_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00539.1"
                     /translation="MKRVHRIARTAAAGMFALGIGMMVACGGSGRPNEDILGDAGDVD
                     QHDVVDELIVPPDNGATDSTHPDSQFDISPGDVVNPTATELVALGMERLSAGESLSAM
                     DAFSAALALQPGHQPALWGMVLARFQSSVAMFSGLTGLVSFRDDPDAEPELAPRSLRT
                     GDLEWPVHEGTIGTSIDALYHAAIDQQQRIAELVAAGGAPFTLETGLPLNFRDDLMMN
                     LCCDWDISDLYGISSFNNLMLAFVSFLGSQETDVSLAVFEETADLAPGISNVLVHVMD
                     NYPDLFALLPEDGATIWQQTGDYLLAACRDALEAARLMAIDNGMTRNVATLSDDEHPY
                     LVLHGSFPGDRDQVEFLWDGVNVSMKDVVTRAEAHLAGTGQPHLSLDEDVLVAIAVFV
                     DIVHRTVGIETLAAGLGFELPDIVTGLIGSLDQNDPEQLAGLLPSLTSLLGIPAGTVY
                     MDVAGFLDHPFNLRDLFPNYGDDPGTEWKTFTRSFECMKAGCTVTGIDSGDAMQFWFM
                     DSTIQADTVELTVSTFPTADTSAAAFETETITLEKVEGFDGLLTGTIVMMGGTEAGPA
                     DDGRIVLPDGGQLVATFVSPTDAAHKVSVTGNMADGFTPWDYGAACGDDDPAWDAAHF
                     WQSEFATAVEVTAPGTTTPLGEVAADGETSRTGFMAFKSPSFNGLLWLKDGDQIAAAD
                     QASFARIFAGVLKALEDF"
     gene            2322..>2977
                     /locus_tag="PKH54_11385"
     CDS             2322..>2977
                     /locus_tag="PKH54_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOD00540.1"
                     /translation="MKKLTLVFILAIATGLAACNPSGEFSSSLSLIPPLEDVIIIEDI
                     SMPSDVFGPADIIPDSVGEITVTDAIADVTVLPRYSYVVVIADTHIKTPDGLPVANAT
                     KIGQEIEKMPWPIEAVFVLGDVCYLLPYTTFQEFMDDPNDRFDIAQEIFENYPVPVYP
                     LIGNHDIDNGPIPLVELGEQLFKHHFGVDPYYAVDVGRWRFLAMSNFKGPTHIVGDPG
                     "
BASE COUNT          578 a          926 c          863 g          610 t
ORIGIN      
        1 aacaatgtca agtatcttgg tcaagattaa agagattgta cgacggatta caccgttctc
       61 aggctcgccc acaggcgact gcggcttacc cgcccttgta cgggcggatt acatccgccc
      121 gccgcggccc tgtaagatgc cttcaagggc tggcatcaca cgtcattaat aggtaagatc
      181 cacccgaaac tttgccccac cctgctttgg aggaaatcaa tgaaacgggt tcatcgaatc
      241 gcgcgcactg ccgccgcggg catgttcgcg cttggaatcg ggatgatggt tgcctgtggc
      301 ggcagcggca ggccaaacga agacatcctc ggcgatgccg gcgatgtcga tcagcatgac
      361 gtcgtcgacg aactgatcgt cccgcccgac aacggtgcga ccgactctac tcatccagac
      421 agccaattcg acatcagccc tggcgacgtg gtgaatccga cggccaccga gcttgttgcc
      481 cttggcatgg aacgtctgtc ggcgggcgaa tcgctgtcgg ccatggatgc attttcagcg
      541 gcgctcgcgt tgcagcccgg acaccagccc gccctttggg gcatggtcct ggcacgcttc
      601 cagtcatccg tcgcgatgtt ctcgggcctg actgggcttg tcagcttccg ggatgacccg
      661 gacgccgaac cggagctggc cccaagatcg ttaaggaccg gcgacctcga gtggcctgtt
      721 cacgaaggca cgatcggcac gtccatcgac gcgctgtatc acgccgccat cgaccagcaa
      781 cagcgtatcg ccgaactggt tgctgccggt ggcgcacctt tcacgcttga aaccggcctg
      841 ccgctgaact tccgcgacga cctgatgatg aacctgtgct gcgactggga tatcagcgac
      901 ctgtacggga tttcgtcgtt caacaacctg atgctggcat ttgtcagctt cctgggttcc
      961 caggagaccg acgtcagtct ggccgttttc gaggaaaccg ccgacctggc gcccggaatc
     1021 agcaacgtcc tggttcatgt gatggacaac tatccggatc tgttcgccct gcttcccgaa
     1081 gacggcgcga cgatctggca gcagaccggc gattacctgc tggccgcctg ccgcgacgcc
     1141 ctcgaggccg cccgcctgat ggcaatcgac aacggcatga caaggaacgt cgcgacactg
     1201 tccgacgacg aacatccata cctggtgctg cacggttcct tcccgggcga ccgcgaccag
     1261 gttgaattcc tgtgggacgg tgtcaacgtc agcatgaagg atgtcgtcac aagggccgag
     1321 gcccatctgg ccggcaccgg ccagccgcac ctgagcctgg acgaggacgt gctggtggcg
     1381 attgccgtgt tcgtcgatat cgtccatcgc acggtcggga tcgaaaccct ggcggcgggg
     1441 ctcggcttcg agcttcccga tatcgtcacc ggcctgatcg gcagccttga ccagaacgac
     1501 ccggaacagc ttgccgggct gcttccgtca ttgacttcac tgcttggcat cccggctgga
     1561 accgtctata tggacgtggc cgggttcctg gatcatccgt tcaacctgcg ggatctgttc
     1621 ccgaattacg gcgatgaccc gggcactgaa tggaagactt tcacaagatc cttcgagtgc
     1681 atgaaggccg gctgcaccgt caccggaatt gattcaggcg acgcgatgca gttctggttc
     1741 atggattcca cgatccaggc cgacacggtc gagctcactg tttccacttt cccgactgcc
     1801 gacacaagcg cggcggcatt cgaaaccgaa acaatcacgc ttgaaaaggt tgaaggattc
     1861 gacggtctcc ttaccggaac cattgtgatg atgggcggca ccgaggctgg gccggccgac
     1921 gacggcagga tcgtccttcc tgacggcggc cagcttgtcg cgacattcgt ttcacccaca
     1981 gacgccgcgc acaaggtttc ggttacaggc aacatggcgg acggcttcac tccgtgggat
     2041 tacggagccg catgcggcga cgacgatccg gcctgggatg cggcccactt ctggcagagc
     2101 gaattcgcga cggcagtcga agtgacggcc cccggcacga cgacaccgct tggcgaggtc
     2161 gctgccgacg gcgaaacgtc gcgaaccggc ttcatggcct tcaagtcgcc ctcgttcaac
     2221 ggtcttctgt ggctgaagga cggcgatcag attgcggccg ccgaccaggc ttcatttgcc
     2281 cggattttcg ccggcgtcct taaggcactg gaggatttct gatgaaaaag ctgacactgg
     2341 tattcattct tgccatcgcc accggacttg ccgcgtgcaa cccttcgggc gagttttcgt
     2401 cctcgctttc gcttattccg ccacttgagg acgtcatcat catcgaagac atttccatgc
     2461 cgtcggacgt cttcgggcca gccgacataa tccccgattc tgttggcgaa atcaccgtca
     2521 cggacgcgat tgcggacgtc acggttctgc cccgctattc gtatgttgtg gtcatcgcgg
     2581 acacgcacat caagacgccg gacggccttc ccgttgccaa cgccacaaag attggtcagg
     2641 aaatcgagaa aatgccctgg ccgatcgagg ccgttttcgt tcttggcgac gtctgttacc
     2701 tgctgccgta cacgaccttc caggagttca tggacgaccc gaacgaccgc ttcgacatcg
     2761 cccaggaaat ctttgagaac taccctgttc cggtttatcc gcttatcggt aaccatgaca
     2821 tcgacaacgg gccgatccca cttgtggaac tgggcgagca gcttttcaag caccacttcg
     2881 gggttgatcc gtattacgcg gtggatgtcg ggcggtggcg cttcctggcg atgagcaatt
     2941 tcaagggtcc gacacacatc gttggcgacc ccggata
//
LOCUS       DAOPPA010000416         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_36739_length_2937_cov_3.997224, whole genome shotgun sequence.
ACCESSION   DAOPPA010000416 DAOPPA010000000
VERSION     DAOPPA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36739_length_2937_cov_3.997224"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2937
                     /locus_tag="PKH54_11390"
     CDS             <1..>2937
                     /locus_tag="PKH54_11390"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOD00541.1"
                     /translation="SACPDGYDCVEATDPETSRKSTQCIPSNGTCTCRLKNDTEQRPC
                     SKDNEAGTCLGFQTCNLATGWSECSAAEPTAEICDGLDNDCNRISDDNPELPVGDCFN
                     TVDGIGSCRGEWLCRGQDGWDCFGPIPAAETCNYLDDNCDGSTDEDFKVDGKYVSEEN
                     CGQCGVGCGGLIPFAKTTSCDPGINPEDVPQCVVEECIQGYLKAGPQLCRPAISSLCV
                     PCEQDSNCGSEVNKCIQFESGKYCGRDCSPDGIYGPECPGGYQCSDVAGSMQCVPISG
                     TCECTQANHGIKRICEIVNEFGTCQGNEVCDGAVGWVNCSARVPADEICDAIDNDCNG
                     LVDDDPIQPPDPCEKSWTDPVSGTEYTCGGTWSCQDTPSGKEWVCPAKMPSAEVCNAI
                     DDNCNGETDEDFKLAGTNKYNNYDHCGACNVSCAGLVQNAVMKCDATGPQPVCLVDYC
                     NEGWWKASDLSCQPFPESQCLACSSDASCQIPGDVCIPADSVGNTYCMWDCSEDALRP
                     ELDPDRKTCPEGYYCRETNDLGQPLFKCFPQSNSCDCLAANAGHVRACVISNSFGRCF
                     GDQTCQPATGWTVCNAQTPSVEICNNNDDDCNGLIDEPFPSVGSTCTAGIGECLGTGN
                     IVCNAVGDGVECTAVAGEPVSEICDLKDNDCDNSVDEDFPDYGKVCFVGTGECLASGT
                     YVCQAGGLGVECNAVQGTPVDETCDDRDNDCDGSVDEDYLLKGRVCAVGVGECFANGV
                     WICSADQSGLACDAIEGLAGQELCDGLDNDCDLEIDELWPERETVCVVGQGACRATGV
                     FRCMADMSGIECSVVAGTPSPEICDGIDNDCDGSIDEDWADLGRACQEGVGECLANGV
                     WTCTADLSGVECSANPGTPVAELCDGKDNDCDGSIDEDWTNKGTACQEGVGECLANGV
                     IVCTADLSGVECSANPGTPVAELCDGLDNDCDGSIDEDWTNKGFACQEGVGECLDTGV
                     IVCTADL"
BASE COUNT          598 a          770 c          998 g          571 t
ORIGIN      
        1 ttcggcgtgt cccgacggtt acgactgcgt cgaggcaacg gatcccgaga cttcaaggaa
       61 gtcaacgcag tgcattccct cgaacgggac gtgcacctgc cgcctgaaga acgatactga
      121 gcagcgcccc tgctcgaagg acaacgaagc cggtacgtgt ctcgggttcc agacgtgtaa
      181 tcttgcaact ggctggagcg aatgttcggc ggcggagccg acggcggaaa tctgcgacgg
      241 actggacaac gactgcaacc ggatttcgga cgacaatccg gaacttccgg tgggcgattg
      301 cttcaatacg gtggacggga tcggttcgtg tcgcggtgaa tggctttgtc ggggtcagga
      361 tggctgggat tgtttcgggc cgataccggc ggccgagacc tgcaactatc tggacgacaa
      421 ctgcgacggc agcacggacg aggacttcaa ggttgacggt aagtacgtca gcgaggagaa
      481 ctgcgggcag tgcggggtcg gttgcggtgg tctgattccg ttcgcgaaga cgacgtcgtg
      541 cgatcccggc atcaatccgg aggatgttcc ccagtgcgtg gtcgaggagt gcatccaggg
      601 atacctgaag gccggtccgc agctttgtcg tccggcgatt tcaagccttt gcgttccctg
      661 tgagcaggat tcgaactgcg gatccgaagt caacaagtgt atccagttcg agtcgggtaa
      721 gtactgcggc cgggactgca gtccggacgg catttacggc ccggagtgtc caggaggtta
      781 tcagtgttcg gacgttgccg gttcgatgca gtgcgttccg atttcgggaa cctgcgagtg
      841 cacgcaggcg aaccacggga tcaagcgaat ctgcgaaatc gtgaatgaat tcggaacgtg
      901 ccagggtaac gaggtctgtg acggggctgt tggctgggtg aactgctcgg ccagggttcc
      961 cgctgacgaa atctgtgacg cgattgacaa cgattgcaac gggctggtcg atgacgatcc
     1021 gatccagccc ccggatccgt gcgagaaatc gtggaccgat cctgtgtctg gaacggaata
     1081 cacgtgcggc gggacgtggt cctgccagga cactccatcc gggaaggaat gggtatgtcc
     1141 cgcgaagatg ccatccgctg aagtctgcaa cgcgatcgat gataactgca acggagagac
     1201 ggacgaggat ttcaagctgg ccggaacgaa caagtacaac aattacgacc actgcggcgc
     1261 gtgcaacgtt tcgtgcgcgg gcctggtgca gaacgcggtc atgaagtgcg acgcgaccgg
     1321 tccgcagccc gtatgcctgg tcgattactg caacgagggc tggtggaagg cgtcggatct
     1381 ttcctgtcag ccgttccccg aatcgcagtg tcttgcgtgc agcagcgatg cgagttgcca
     1441 gatcccgggt gacgtgtgca tcccggcgga ctcggttgga aatacatact gcatgtggga
     1501 ttgcagcgag gacgcattgc gtccggaact tgatcccgat cgtaagactt gtccggaagg
     1561 ttattactgc cgcgagacga acgacctggg ccagcccctt ttcaagtgtt tcccgcagtc
     1621 gaattcgtgc gactgccttg ccgcaaacgc gggtcatgtc cgtgcatgcg tcatttcgaa
     1681 ttccttcggt cggtgctttg gcgatcagac gtgtcagccg gcgaccggct ggaccgtgtg
     1741 caatgcccag accccgtcgg tggaaatctg caacaacaac gacgatgact gcaacggcct
     1801 gatcgacgag cccttcccgt cggtcggttc gacgtgcacg gccggtatcg gggaatgcct
     1861 tggcactgga aatatcgtgt gcaacgcggt tggcgacggc gtggagtgca cggctgttgc
     1921 gggcgagcct gtttccgaaa tctgcgacct gaaggataac gactgcgaca actcggttga
     1981 cgaggacttc ccggactacg gaaaggtgtg tttcgtcggg acgggcgagt gccttgccag
     2041 cggaacctat gtatgccagg cgggcggcct gggcgtggaa tgcaacgccg tccaggggac
     2101 tccggtcgat gaaacctgtg atgacaggga taacgactgc gacggcagcg tcgatgaaga
     2161 ctacctgctg aaggggcggg tctgcgccgt gggcgtgggc gagtgcttcg cgaacggtgt
     2221 atggatctgc agtgcggatc agagtggact ggcgtgcgat gcgatcgagg gacttgcagg
     2281 ccaggaactt tgtgacggcc ttgataacga ctgcgacctt gagatcgacg aattatggcc
     2341 ggaacgggaa accgtatgcg tggtcggcca gggcgcgtgc cgcgcgacgg gcgttttccg
     2401 atgcatggcc gatatgtcgg gcattgaatg cagcgtggtt gcaggtaccc catcgcccga
     2461 aatctgcgac ggaatcgaca acgactgcga cggcagtatc gatgaagact gggccgatct
     2521 gggtcgcgcc tgccaggaag gcgtgggtga atgccttgcg aacggcgtct ggacctgtac
     2581 cgctgatctg agcggggttg aatgcagcgc caacccggga acgcctgttg ccgaactgtg
     2641 cgacggcaag gataacgact gcgacggcag catcgatgaa gactggacga acaagggcac
     2701 ggcctgccag gaaggcgtgg gtgaatgcct tgcgaacggc gtcatcgtct gtacggcgga
     2761 tctgagcggc gtcgaatgca gcgcgaatcc tggaacgcct gttgccgaat tgtgcgacgg
     2821 ccttgacaac gactgcgacg gcagcatcga tgaagactgg acaaacaagg gttttgcctg
     2881 tcaggaaggc gttggcgaat gccttgatac gggcgtcatc gtctgcacgg ccgatct
//
LOCUS       DAOPPA010000417         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_36954_length_2922_cov_4.015696, whole genome shotgun sequence.
ACCESSION   DAOPPA010000417 DAOPPA010000000
VERSION     DAOPPA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36954_length_2922_cov_4.015696"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /gene="hxsB"
                     /locus_tag="PKH54_11395"
     CDS             <1..1435
                     /gene="hxsB"
                     /locus_tag="PKH54_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HOD00542.1"
                     /translation="NDAGEFVVLTPAEMDLVASNKVKPGSELENRLLAKHMLMCGPDC
                     IEEAAEAFGRKASYLYRGPVLHIMVVTLRCNQVCKYCHASRAGMKETENDMSIATAEK
                     VVDMAMRSPSDSITIEFQGGEPLVNWEVVKHVVEYGTMKADAAGKRVMFSLVSNLSMM
                     DDEKLDYLVRRRVQISTSLDGPKDIHDGNRKLSGASAYDKCVFWMERINKAYVAAGLD
                     PKLYHVEALATITREHLGRARDLVDEYIARGCRAIFMRPLNPFGYAKQTEQSISYSAD
                     DFLKFYDEALKYILEQNRKGVEVMERLAAIFLTKIMTPDDPNYLDIRNPCGAGVGQIA
                     YNHDGSIFTCDEGRMVYQMGDDIFKLGEIDNMTLKDVVTHETVKTLMMASTLDSIPGC
                     ATCAYKPWCGTCPVYNYGEQGNIFPQNSTNQRCRIYMGVQDRLMKYFVDGDNEVIDIF
                     RRWVEVKERPEYLHDGRPKADDGSRGV"
     gene            1435..2358
                     /locus_tag="PKH54_11400"
     CDS             1435..2358
                     /locus_tag="PKH54_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00543.1"
                     /translation="MSAALPNPAVKAVLKITYKCNSRCLFCRASDYRDPVADEPAETI
                     LKKAMMAKARGAAMILFSGGEPTLRRDLFLLAKGVRALGMDFGLITNGAMLANREYLD
                     RLLELGLKYVHTSLHGSTAATHSRLTCAHTFEPVMAAIERLRGTGVELHVNTVIALAN
                     IDDLAAIGDLLAPFAPITHKLCLAEPRGFFEENEAELLVQPELAGRAACQEVIRTAAA
                     YPETGFKTVVEGFPLCQIRTALDSVSNLETHNIIFMSEGFEDEIYRSDHGQREFPAIC
                     DGCAMRPSCPGVYPGYVRRFGVVGLRAFRQR"
     gene            complement(2413..>2922)
                     /locus_tag="PKH54_11405"
     CDS             complement(2413..>2922)
                     /locus_tag="PKH54_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00544.1"
                     /translation="EIPRIEIPAPVPDGVSLDTVSEDVFDGTVTFRPVADYTTPDLYV
                     LRLNYLTAAPRNTFCGDRESGLSCSLGGPRSHALWEMFVPPDMTTVTLPTFPPGFPTP
                     YLGNPDPTPAASTSPHRFDADTIEVELSAYVLGADGKPFDYDDNFAYQDVNMHCTVVS
                     QDSVAVRVK"
BASE COUNT          630 a          847 c          917 g          528 t
ORIGIN      
        1 caacgacgcg ggcgagttcg tcgtgctgac tccggccgag atggatctgg tggcttccaa
       61 caaggtgaag ccgggaagcg aacttgaaaa caggctgctt gccaaacaca tgctgatgtg
      121 cggccccgac tgcatcgagg aagccgcgga ggctttcggc aggaaggctt cctatctgta
      181 ccggggcccg gtcctgcaca tcatggtcgt gaccctgcgc tgcaaccagg tgtgcaagta
      241 ctgtcacgcc agccgcgccg gcatgaagga aaccgaaaac gacatgtcga ttgccaccgc
      301 cgaaaaggtg gtcgatatgg cgatgcggtc gccttcggac agcatcacga tcgaattcca
      361 gggcggcgag ccgctggtca actgggaagt cgtcaaacat gtcgtcgaat acgggacgat
      421 gaaggccgat gccgccggca agcgggtgat gttcagcctt gtgtcgaacc tgtcgatgat
      481 ggatgacgag aagctggatt acctggttcg caggcgggtc cagatttcca cgtcgcttga
      541 cggcccgaag gacatccacg acggcaaccg caagctgtcg ggcgccagcg cttacgacaa
      601 gtgcgtattc tggatggaac gcatcaacaa ggcttacgtg gcggccgggc tggatccgaa
      661 gctgtaccac gtcgaggccc ttgcgacgat caccagggaa cacctgggac gcgcccggga
      721 cctggtcgat gaatacatcg cccgcggctg ccgcgccata ttcatgcggc cgctgaaccc
      781 gttcgggtat gcgaagcaga cggagcagtc gatttcgtat tccgccgacg acttcctgaa
      841 gttttacgac gaggcgctga agtacatcct cgagcagaac cgcaagggcg tcgaagtgat
      901 ggaacgcctt gccgcgatat tcctgacgaa gatcatgacg ccggacgacc cgaattacct
      961 ggacatccgc aacccgtgcg gcgccggggt cggccagatt gcgtacaacc acgacggttc
     1021 gatattcacg tgcgacgaag gccgcatggt ctatcagatg ggcgacgaca tcttcaagct
     1081 gggcgaaatc gacaacatga cgctgaagga cgtcgtgacg cacgagaccg tcaagactct
     1141 gatgatggct tcaacgctgg attccatccc cggttgcgcc acttgcgcgt acaagccgtg
     1201 gtgcggaacg tgcccggttt acaactacgg cgagcagggc aacattttcc cgcaaaacag
     1261 cacgaaccag cgatgccgga tttacatggg cgtccaggat cgcctgatga agtatttcgt
     1321 cgatggggac aacgaagtca tcgatatctt caggcgctgg gtcgaggtga aggaaaggcc
     1381 cgaatacctg catgacggca ggccgaaggc cgatgacgga tccaggggcg tctgatgtcc
     1441 gcggcactgc cgaacccggc cgtcaaggcc gtcctgaaaa tcacctacaa atgcaattcc
     1501 cgctgcctgt tctgcagggc gtccgactat cgcgacccgg tcgcggacga accggccgaa
     1561 acgatcctga aaaaggccat gatggcaaag gcccggggtg ccgcgatgat cctgttttcc
     1621 ggcggcgagc cgacgctgcg acgcgacctt ttcctgctgg cgaagggcgt gcgggccctt
     1681 ggcatggact tcgggctgat aacgaacggg gcgatgcttg ccaacaggga atacctggac
     1741 aggctgctgg aactggggct gaagtacgtc catacgtcgc ttcacggttc gacggccgcg
     1801 acgcattcgc gactgacgtg cgcgcacacg ttcgaaccgg tgatggcggc catcgaacgc
     1861 cttcgcggaa ccggggtgga actgcacgtc aacacggtga ttgcacttgc caacattgac
     1921 gatctggcgg ccatcggcga cctgctggcg ccgtttgcac cgatcaccca caagctgtgc
     1981 cttgccgagc cgcgcggctt cttcgaggaa aacgaggccg aactcctggt gcagccggaa
     2041 cttgccggac gcgccgcctg ccaggaagtc atccggaccg cggccgcata tccggaaacg
     2101 ggctttaaga ctgtcgttga aggcttcccg ctttgccaga tacgaaccgc gctcgattcg
     2161 gtttcgaacc ttgaaacgca caacataatc ttcatgagtg aaggcttcga ggatgaaatt
     2221 taccgttccg accacggtca gcgggaattt ccggccatct gcgacggatg cgccatgcgg
     2281 ccgtcgtgcc ccggcgtcta tccgggatac gtcagacgtt tcggcgtcgt gggactgcgg
     2341 gcattcaggc agaggtgatc tcttacagtc aggccggccg gattcgtcgg ccagggacat
     2401 gaaaaccgcc ggtcatttca cgcgaaccgc caccgaatcc tgcgaaacga ccgtgcagtg
     2461 catgttcacg tcctgatagg cgaaattgtc atcgtaatca aaaggctttc cgtcggcccc
     2521 cagaacatag gcgctcaatt cgacctcgat ggtgtccgca tcgaagcgat ggggcgaagt
     2581 ggaagcggcc ggcgtgggat ccgggttccc aaggtaaggg gttgggaatc ccggcgggaa
     2641 tgtcggcaat gtgactgtcg tcatgtccgg tggaacaaac atctcccaaa gggcgtgcga
     2701 ccgtggtccg ccaagtgaac acgacaagcc ggactcgcga tcgccgcaga aggtattcct
     2761 tggggccgcg gtcaggtagt tcaggcgcag cacgtaaaga tctggggtcg tgtagtccgc
     2821 caccggcctg aaggtgacgg tgccgtcaaa gacgtcctcg gaaacggtat cgagcgaaac
     2881 accgtcaggc accggggctg gaatctcgat cctgggaatc tc
//
LOCUS       DAOPPA010000418         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37201_length_2909_cov_3.719692, whole genome shotgun sequence.
ACCESSION   DAOPPA010000418 DAOPPA010000000
VERSION     DAOPPA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37201_length_2909_cov_3.719692"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKH54_11410"
     CDS             <1..453
                     /locus_tag="PKH54_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00545.1"
                     /translation="IEAIAPVGEGQEIDTYHGNPGILYPMVFAGDPSTFTLFDGTVLG
                     QGWVDDGVYELTFSDGLEFTQGAIFDVVGLGDDVPVMMMDGIELAYFAMPDELEEAEY
                     GFCQLFGHTLVKVPGGERKVTITFLAADRPGFHPDWSDIFRVRLTSSQ"
     gene            595..2844
                     /locus_tag="PKH54_11415"
     CDS             595..2844
                     /locus_tag="PKH54_11415"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOD00546.1"
                     /translation="MSGFFRCFFCGVVFAGMLALSACGDGGASDDTIGLDFLHGFDIN
                     YGDIPVGYDVKSDIPVVGQCTGDDNCPREKPHCLIARGECVECIQHSHCMGRGFCLEG
                     FVCGVTECQPDEAICVKNVAKVCNEDGSKQTVTDCGEDKYCVEGACYACPPLLGDCPS
                     EKVARRCKADGSGWEEETCGNGLTCANGDCLSCIPGETICDGSTKVRKCTMDGSGFQE
                     HVDCWDAHEGDICHMGQCINLCEFTSKFYTNKGCEYYAVDMEQFADSDPPYYGEDAPF
                     AIVVSNTFEALDARVVVYHGDTPVKTLNAPAGLATIINLEPLNINGVGITDKTYHVKS
                     NLPIVAYQFNPLENVEVFSNDASLLLPTNVLGRSYRVMAMPSTGMNGAGQFLTGLITI
                     VGVEEGDTLIKVTPTTSLFAGDGIPAIAKNATGEFTLKKGQVANLKTDYVAYSDLTGT
                     LVEADKKIAVFSGHTCAYAPVSTCAAGKCSFDKSVSCSTQASCPWVMACDHLEEQIQP
                     LAAWGKEYVIPKSKSRGKAPDLVRVVANEANTRVTITPPIAGTKVLGPGEYYEFEIDR
                     SVVVKSDNQFLAAFVLEGEDAPGSAHEICINSTLDDFCHNGSGFGCMCTINDGSSVGS
                     ESCKVNADCSPDDANVGDPSLIMAVPVEQYRDFYIFLVPTKYAYSYISVVAPTGANVK
                     IDGTALAASEFTAISGSGFGTADKHMNPGSHTLESDKPVGLYVYGWDSYVSYGYPGGM
                     MLIKLAGDI"
BASE COUNT          592 a          799 c          884 g          634 t
ORIGIN      
        1 atcgaggcca ttgccccggt gggcgaaggc caggaaatcg acacttatca cggcaatccc
       61 ggaatcctct atccgatggt gtttgcgggc gaccccagca ccttcaccct gtttgacggt
      121 accgtcctgg ggcagggctg ggtcgatgac ggcgtgtacg aactgacctt cagcgacggc
      181 ctggaattca cccagggagc gatttttgac gttgtcggcc tgggagacga tgtcccggtc
      241 atgatgatgg acggaatcga gttggcctac ttcgcgatgc cggacgaact ggaggaggcc
      301 gagtacggtt tctgccagct tttcgggcat accctggtca aggttcccgg tggcgagcgc
      361 aaggtcacga tcaccttcct ggcggctgat cgaccaggat ttcacccaga ctggtctgac
      421 atcttccgcg tccgtttgac ttcatcacag tgatttgcga cggcttgccc cgaaatttgt
      481 cggaagtggg cttgaacggc ccgaaaatcg gtaaactttc ggcggaaaca atgtttggca
      541 aaaacgatgt ttactgtatt atcacggcat gtagttattc gcccgggggt cttcatgtcc
      601 ggtttcttca gatgtttctt ctgcggtgtt gtgtttgccg gaatgctggc gttgtcggcg
      661 tgtggtgacg gcggtgcttc ggacgacacc atcgggcttg atttcctgca cggctttgac
      721 atcaattacg gcgacattcc agtcggatat gatgtcaagt ctgatattcc ggttgtgggc
      781 cagtgcaccg gcgatgacaa ctgcccgcgt gaaaagccac attgcctgat tgcccggggc
      841 gagtgcgttg agtgcatcca gcattcacat tgcatgggca ggggattctg tctcgagggt
      901 tttgtttgcg gtgtcaccga atgccagcct gacgaggcga tttgcgtgaa gaacgtcgcc
      961 aaggtctgca acgaggacgg cagcaagcag acggtcaccg attgcgggga agacaagtac
     1021 tgtgtcgagg gcgcctgtta cgcttgccct ccgctgcttg gggattgccc ttccgagaag
     1081 gttgccaggc gctgcaaggc cgatggctcg ggctgggaag aggaaacgtg cggaaatggc
     1141 ctgacctgcg cgaatggcga ctgcctgtca tgcattcccg gtgaaaccat atgtgacggc
     1201 tcgaccaagg ttcgcaaatg cacgatggac ggttcgggct tccaggaaca tgtggattgc
     1261 tgggatgccc acgaaggcga catctgccac atggggcagt gcatcaacct gtgcgaattc
     1321 acctcgaagt tttacacgaa caagggctgc gagtactacg cggtcgatat ggagcagttc
     1381 gcggacagtg atccgccgta ctacggcgag gacgctccgt tcgcgatagt tgtttccaac
     1441 actttcgagg cgctggacgc aagggttgtc gtctatcatg gcgacacgcc ggtcaagaca
     1501 ctcaacgcac cggccggcct ggcgaccatc atcaatctgg agccgctcaa catcaacggc
     1561 gtgggcatca cggacaagac gtaccatgtg aagtcgaacc tgccgatagt tgcgtatcag
     1621 ttcaatccgc ttgaaaacgt cgaggtcttt tccaacgacg cgtcgctgct gttgcccacc
     1681 aatgtccttg gccggagcta tcgcgtcatg gcgatgcctt cgacaggcat gaacggggct
     1741 ggccagttcc tgacggggtt gatcacgatt gtcggcgtcg aggaaggcga cacgcttatc
     1801 aaggtgaccc cgaccaccag cctgttcgcc ggtgatggca ttcccgcgat cgcaaagaac
     1861 gcgaccggcg agttcacgtt gaaaaagggc caggtcgcca atctgaagac cgattacgtg
     1921 gcatattccg acctgaccgg cacgctggtc gaggccgaca agaagatcgc ggtgttttca
     1981 ggccatacct gcgcatatgc gccggtttcg acctgtgccg ccggcaagtg cagttttgac
     2041 aagtcggtct cgtgcagtac ccaggcgtcg tgtccgtggg tgatggcgtg cgaccatctt
     2101 gaagagcaga tccagcctct ggccgcctgg ggaaaggaat acgtcattcc gaaaagcaag
     2161 tcccgcggca aggccccgga tctggttcgc gtcgttgcaa acgaggccaa cacccgtgtg
     2221 acgattacgc cgccgattgc cggtaccaag gttcttggcc ctggtgagta ctacgagttt
     2281 gagatcgaca gatccgttgt cgtgaagtcc gataaccagt tcctggcggc atttgtcctt
     2341 gagggcgagg atgcgccagg ttcggctcat gaaatctgca tcaattcgac gttggacgat
     2401 ttctgtcaca acggcagcgg cttcgggtgc atgtgcacga tcaatgacgg atccagcgtc
     2461 ggcagcgagt cgtgcaaggt caatgcggac tgcagcccgg acgatgcaaa cgttggcgac
     2521 ccgtcgctta tcatggcggt tccggtcgag cagtaccgcg acttctacat cttcctggtt
     2581 ccgacaaagt atgcctacag ctacatctcc gtggtggccc ccaccggcgc gaacgtgaag
     2641 attgacggga cggcccttgc ggcgtccgaa ttcacagcga tttccggcag cgggttcggc
     2701 acggctgaca agcacatgaa cccgggctcg cacacccttg aatccgacaa gcccgtcggc
     2761 ctgtacgttt atggctggga cagctatgtt tcgtacggtt atccgggcgg aatgatgctc
     2821 atcaagcttg ccggcgacat ctagcatttc caccgctaaa agtgtctcac gcagacgggc
     2881 aaattctcac gcaggcgggc gaattctca
//
LOCUS       DAOPPA010000419         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37284_length_2904_cov_3.712180, whole genome shotgun sequence.
ACCESSION   DAOPPA010000419 DAOPPA010000000
VERSION     DAOPPA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37284_length_2904_cov_3.712180"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKH54_11420"
     CDS             complement(<1..350)
                     /locus_tag="PKH54_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00547.1"
                     /translation="MSRNSLAVMLLAACSILSGCAGSNGPGDGSIDQISDDGAWDATP
                     VDAEADSVVRDALPEAWTDAGTDVADTDQDLFRPEVIDTDGTDDIEPDAVDAGADVAG
                     GQRVVSVHDLAVQAA"
     gene            complement(396..2591)
                     /locus_tag="PKH54_11425"
     CDS             complement(396..2591)
                     /locus_tag="PKH54_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00548.1"
                     /translation="MPADRIPTMTRLAVMCLGAQSTLAQVALLRAGLERFGGNEFFMG
                     VALAAWLAAGALGAALFGRIGDRRGGGRILAAIGSGMVTAGMGIGLIILSTLSVRFSV
                     TGMAMHPLAAMAWMAAATAPAAVVSGVLFAWLVPMLAATYGAGLGRTTILESVGAGIG
                     GLVVGSLAVEWIGTPGLVAAVALTGGFAALGLAGIKRLPCRVATIAGTLVVAAACAWA
                     SWLIVPAPAGSVIISRVENRFGRRAAVVFDRQKTVVQGPSTVIDCPDRRTPEILATMV
                     KGLSPEAHRILAAGGTSHDLALLASIPGTDVTLMTRDIALDNLDRDFCRSDRDRAVTV
                     IPGDFLYEISGVSAGLDAIILRVGPPATLDRTGLVTSNTFWKASAALKRDGRLFVTLG
                     AAESHPTDAAIRSLASILNAARGAFPECRLLPFADWLLTCGRTAPDLNAIKAAVDVLP
                     FERMHATDAFIDDVLDPFELEQLDRRIESVRRDVPANSAILPAAQLYTLLDWAGRHNP
                     SVATAASTPGRLPAAAACILFPAIIMVLALSRRLRGRMASAYATSAVTGICGMTVEFA
                     LMWAIQSSWGSLAGWLGGIIAAYMGGLGAGAWVAWRLMDRRGASPTRTMKAFNAGAAI
                     STIATIPVVLLAASGIPSAAGLVLILVLAFTTAAFCGGAFQSAAQGLRDSSALGTCDL
                     TGRIRAVDTMAAATAAIACPILLFPIIGVVAPVAASGVACFMLALHKID"
     gene            complement(2596..>2904)
                     /locus_tag="PKH54_11430"
     CDS             complement(2596..>2904)
                     /locus_tag="PKH54_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOD00549.1"
                     /translation="DFVLVRTDELGAIEYEISVLFPAVPVSDVNEIVVGRDGLIIQLG
                     SRRGLLLPQVPVEWGWNRQQFLDHTARKAGLPMDAWKNPDAKLFRFEGIIFNEHDVGK
                     "
BASE COUNT          568 a          983 c          903 g          450 t
ORIGIN      
        1 gccgcttgca cggccaggtc gtgcacgctg accacgcgct gcccgccggc aacgtccgcg
       61 ccggcgtcca cggcgtccgg ttcgatgtcg tctgtcccgt ccgtatcgat gacttcgggc
      121 cggaacagat cctggtccgt gtccgccacg tcggttccgg catccgtcca cgcttccggc
      181 agcgcgtccc ggaccacaga gtccgcttcc gcgtcaaccg gcgtcgcgtc ccaggcgcca
      241 tcatccgaaa tctggtcaat cgacccgtcg ccaggcccgt tggatcccgc gcatcccgaa
      301 agaatcgaac aagccgcaag caacatgact gccagggaat tccgtgacat cgaaacaacc
      361 ccgtcgcgca aacagatgtt gcaagtctag gacaatcaat cgattttgtg cagtgcaagc
      421 atgaagcagg caacgccgga tgccgccacc ggggccacga cgccgattat cgggaagagc
      481 aggatcgggc acgcgattgc cgccgttgcg gccgccatgg tgtcaacggc cctgattcga
      541 ccggtcaggt cgcatgtgcc aagcgcgctg gaatcccgca gcccctgggc agccgactgg
      601 aaggcaccac cacagaatgc ggctgtcgtg aaggccagaa ccagaatcag cacaaggccg
      661 gcggcggaag gaatgccgga ggccgcaagc agcacgaccg ggattgtcgc gattgtcgat
      721 atcgccgcgc cggcgttgaa ggccttcatc gtgcgggtcg ggcttgcccc cctgcgatcc
      781 atcaggcgcc acgcgaccca ggcaccggca ccaagcccgc ccatgtatgc tgcgatgatt
      841 ccgccaagcc agccggccag cgagccccac gacgactgaa tcgcccacat cagcgcgaac
      901 tcgacggtca tgccgcagat tccggttacc gccgatgtcg cgtatgcaga tgccatacgt
      961 ccgcgaaggc gtctggaaag cgcaagcacc atgatgattg ccgggaacag gatgcaggcg
     1021 gccgccgctg gaagccgccc aggggtcgag gcggccgtgg cgaccgacgg gttgtgcctg
     1081 ccggcccagt ccaggagcgt gtaaagctgg gccgccggca ggatcgcgct gttggccggc
     1141 acgtcacgcc tgaccgattc aatcctgcga tcaagttgtt ccagttcgaa cgggtcaagc
     1201 acgtcgtcaa tgaaggcgtc ggtcgcatgc atccgttcga atggaagaac gtccaccgcg
     1261 gccttgatcg cattcagatc cggcgccgtc cggccgcatg tcagcagcca gtccgcgaat
     1321 ggaagcaacc ggcattcggg aaaagcaccc cttgccgcgt tcagtatcga cgcaagtgaa
     1381 cggattgccg catcggttgg atggctttcg gccgccccga gcgtcacgaa caggcggccg
     1441 tcgcgcttca acgccgcgga cgccttccag aacgtgttcg aagtgaccag gccggtcctg
     1501 tcgagcgtcg ccggcggccc cacgcgcagg atgatcgcgt cgagaccggc agacacgccg
     1561 gaaatctcgt aaaggaaatc cccggggatc acagtgacgg ccctgtcgcg atcgctgcgg
     1621 cagaagtccc gatccaggtt gtccagcgcg atgtcgcggg tcatcagagt gacgtccgta
     1681 cccgggatgg aagccagcag ggccaggtcg tgcgacgtcc cgcccgcggc aaggatgcgg
     1741 tgggcttcag gcgaaaggcc cttgaccatc gtggccagga tttccggcgt gcgcctgtct
     1801 gggcagtcga taacggttga gggtccctgg accacagtct tctgtctgtc gaataccacg
     1861 gcggcgcggc ggccgaaccg gttttccacc cttgaaatga tgaccgagcc tgcaggcgcc
     1921 ggaacaatca gccaggatgc ccaggcacac gcagccgcga cgacaagggt tcccgcgatg
     1981 gttgcgacgc ggcaaggcag gcgctttatc ccggcaagtc ccagggcggc gaaaccgcct
     2041 gtcaaggcga ccgcggccac cagcccgggc gtgccgatcc attccacggc cagtgacccg
     2101 acaacaaggc cgccgatgcc ggcccccacc gattcaagga tcgtggttcg tcccaggccg
     2161 gcgccgtagg tcgcggccag catcggcaca agccaggcaa acagcacgcc ggaaacgacg
     2221 gccgccggtg ccgtcgcggc cgccatccag gccatcgccg ccagggggtg catcgccatt
     2281 ccggtgaccg agaagcgaac cgagagcgtc gaaaggatta tcaggccgat gcccatgccg
     2341 gcggtaacca tcccggatcc gattgccgcc aggatccggc cgccgccgcg ccgatcgccg
     2401 atccttccaa acagggccgc tcccagggca ccggccgcaa gccaggccgc cagagcgaca
     2461 cccatgaaaa attcgtttcc gccgaagcgc tccaggccgg cacgcagcaa tgccacctgg
     2521 gcgagcgtgg attgcgcgcc caggcacatc acggcgagcc ttgtcatggt cggaattctg
     2581 tccgcgggca cccggttact tcccgacatc gtgttcattg aagatgattc cttcgaagcg
     2641 gaaaagcttt gcgtccgggt tcttccaggc atccatcggc agcccggcct tgcgggctgt
     2701 atggtcaagg aactgctgcc tgttccatcc ccattcgaca ggcacctgtg gcagaagcag
     2761 tcccctgcgg gaaccaagct ggataatcag tccgtcccgg ccgaccacga tttcgttcac
     2821 gtcggatacg ggcaccgccg gaaacaggac gcttatctcg tattcaatcg ccccgagttc
     2881 atccgtccgc accagaacga aatc
//
LOCUS       DAOPPA010000420         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37380_length_2898_cov_3.437918, whole genome shotgun sequence.
ACCESSION   DAOPPA010000420 DAOPPA010000000
VERSION     DAOPPA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37380_length_2898_cov_3.437918"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKH54_11435"
     CDS             <1..715
                     /locus_tag="PKH54_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00550.1"
                     /translation="WGCFDWEDIAVGPFGPEGASHIFVADSGGNGCKDRTSYRLFIVA
                     EPPVLAEGSISEVTVVTYVYPDGPHDCEAVFVDPVDGSVYFVVKEERIKTSGVYRLSA
                     QDLAEAGTDPVTATLVATIPGQLVTGADMSEDGRMLVIRNYGGQKILDTLCGGLLFER
                     GDGEPVADMLGGTFCLLPDFSGEPWIEIQGEAIAISSDGAGLLTAPEYILGGFVPQLL
                     RFWPFSHSDRSRPGPFSSR"
     gene            772..1563
                     /gene="pssA"
                     /locus_tag="PKH54_11440"
     CDS             772..1563
                     /gene="pssA"
                     /locus_tag="PKH54_11440"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOD00551.1"
                     /translation="MDAKNAKYILPNLFTLASVLFGLVSVTYTMAGTPGDFTRAAICI
                     LLAMVADSFDGRVARMTGTSTKFGIQLDSLADALSFGMAPGLLAWSFSLNALGEGRAI
                     PGLIIVFAYVSCGIMRLARFNVSASGKGKKSNYFQGLPIPGGAGIIATMVWSLQDLGI
                     TGADKVGLIAFVMLCLSFLMVSNVPYRNFKHVRISLGLRVLMILAAALVIVAAFKTRT
                     SYVLAIMGMVYVVMGPVEGIVQLFRKGSRRRNRQESDDDADSPEG"
     gene            complement(1627..2556)
                     /locus_tag="PKH54_11445"
     CDS             complement(1627..2556)
                     /locus_tag="PKH54_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOD00552.1"
                     /translation="MDYRSLADTEIQVSAVCFGTWTIGGADWGRTDDRDSAKAIARAF
                     DLGITTFDTAPIYGSGHAEEILGTTLREVRDQVVIATKCGPWEDELGRLRLDLSRKGV
                     FSQCEASLKRLRTDRIDLLQVHWNDPAWPVADTMETLNLLKQSGKIRAFGVSNFSLAE
                     LRQALSGGAISLQSQYGLLSTDIERDCLAECARAGAGFLAYEPLGRGLLAGRYTATTR
                     FQDGDIRNTDARFKGAAFKRNLERVARLKVVAESLGISPAQAAIAWVLHNPAVTTAIC
                     GIRTAAQAVADAGAADIRLDDSSARKLRAIFGG"
     gene            2698..>2898
                     /locus_tag="PKH54_11450"
     CDS             2698..>2898
                     /locus_tag="PKH54_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00553.1"
                     /translation="MPIRMQACLFSFLAVLAIQPGLSAQELAPEPGVVSGQPADEAAP
                     PAASSEPAAPADEAAPPEMVEPS"
BASE COUNT          508 a          886 c          870 g          634 t
ORIGIN      
        1 atggggatgc ttcgactggg aggatatcgc ggtcggcccg ttcggcccgg aaggtgcaag
       61 ccatatcttt gtcgccgaca gtggcggtaa cggctgcaag gatcgaacct cgtaccgcct
      121 ttttatcgtc gccgagccgc ccgtccttgc ggagggatcc atttcagaag taaccgtcgt
      181 gacgtatgtc tatccggacg gaccccatga ctgcgaggcg gtcttcgtcg atccggtcga
      241 tggttcggtg tatttcgtgg tcaaggaaga gcggattaag acgtccggcg tctatcgcct
      301 gtccgcgcag gacctggctg aagccggaac cgatcctgta accgccacgc tggtcgcgac
      361 catcccggga caacttgtca ccggcgccga catgtccgag gacggcagga tgctggtcat
      421 caggaactat ggcggacaga agatcctgga tactttgtgc ggcggcctgc tttttgaacg
      481 gggtgacggc gaaccagtcg cggacatgct gggtggcacg ttctgtcttc ttcccgattt
      541 ttccggcgaa ccctggatcg agattcaggg ggaggccatc gccatttcgt cggacggcgc
      601 cggtctgctg acggctccgg agtatatcct tggaggattt gttccccagt tgctgcgttt
      661 ctggcccttc tcccactccg atcgctcccg gccggggcca ttttccagcc ggtgacgcct
      721 tgcgccatta ttccttttga accggccgcg atgtgttaca atatcgcccg tatggacgca
      781 aagaatgcca agtacatcct gccgaacctc tttacgcttg ccagcgttct gttcgggctt
      841 gtttccgtga catacacgat ggccggcact ccgggcgatt tcacccgagc cgccatctgc
      901 atccttcttg cgatggtcgc cgattcgttc gacggccgcg ttgccaggat gaccgggaca
      961 tcgaccaagt ttggaatcca gctcgattcc cttgcggacg cgctgtcatt cggaatggct
     1021 cccggcttgc tcgcatggtc gttttccctg aatgcacttg gtgaaggtcg cgccattccg
     1081 ggactgataa tcgtgttcgc gtacgtgtct tgcggaatca tgcgccttgc ccgcttcaat
     1141 gtgtccgcgt cgggcaaggg caagaagtcc aattacttcc agggactgcc gattccgggc
     1201 ggtgcgggca tcatcgcgac gatggtctgg tccctgcagg atctgggaat aaccggcgcc
     1261 gacaaggtcg ggcttatcgc tttcgtgatg ctctgcctca gtttcctgat ggtcagcaac
     1321 gtcccgtacc gcaacttcaa gcacgtcagg atcagccttg gacttcgcgt cctgatgatc
     1381 ctggccgccg cactggtcat tgtggcggcc ttcaagaccc gcaccagcta tgtccttgca
     1441 atcatgggca tggtctatgt cgtgatgggt ccggttgaag gcatcgtcca gcttttcagg
     1501 aagggttccc gtcgccggaa tcgccaggaa agcgatgatg acgccgactc ccccgaaggc
     1561 tgaaattcgg ccgtcgcatt cgaatatgga agtcttccat tgccgtttcc cgttgcagaa
     1621 aagtcttcag ccgccaaaga ttgcgcgcag tttgcgggcg gaggagtcgt caagcctgat
     1681 gtctgcggcc ccggcgtcgg cgaccgcctg tgccgccgtt cttataccgc agatggccgt
     1741 ggtgaccgcc gggttgtgaa gaacccaggc aatcgcggcc tgggccggtg aaattccaag
     1801 cgactccgcg acaaccttca acctcgcgac gcgttccagg ttccgtttga aggcggcgcc
     1861 cttgaatcgc gcgtcggtgt tgcgaatgtc gccgtcctgg aacctggtcg tcgccgtgta
     1921 acgtccggcc agcaggcccc tgccaagcgg ttcgtacgca aggaagccgg cgccggcccg
     1981 ggcgcactcg gcaaggcagt cgcgctcgat gtcggttgaa agaaggccgt actgggactg
     2041 aaggctgatt gcgccgcccg ataaagcctg gcgcagttcc gcaaggctga aattcgaaac
     2101 tccgaaggcc ctgatctttc cgctttgctt cagcaggtta agtgtttcca tcgtgtcggc
     2161 gacaggccag gccgggtcgt tccagtgaac ctgaagcagg tcgatgcgat ccgtccgcag
     2221 gcgcttgagg ctggcttcgc actgggaaaa gacgcccttt ctggaaagat ccaggcgcag
     2281 tcttccaagc tcgtcttccc acggcccgca cttggtggca atgacgacct ggtcgcgcac
     2341 ctcccggagg gtcgttccca ggatctcctc ggcatggccg ctgccataga ttggcgccgt
     2401 gtcgaacgtg gtgattccaa ggtcgaacgc acgcgcaatc gcttttgccg aatcgcggtc
     2461 atccgtcctg ccccagtcgg cgccgccaat tgtccatgtg ccgaaacaca ctgccgaaac
     2521 ctgaatctcc gtatccgcca gacttcgata atccatcagg tcaatccccg caaatcgtcg
     2581 attccgatgt taccagcacg tcgggaacgc gggcgggtaa attgacaccg ggacgcacag
     2641 acacctagaa ttgcgaaagt tcgagatttc tctcgcggtg gtttccgggg gttaacgatg
     2701 ccaatccgca tgcaggcatg ccttttctct ttcctggccg ttcttgcgat tcagcccggc
     2761 ctttcggccc aggaactggc gcccgagcct ggcgtggttt ccgggcagcc ggcagatgag
     2821 gcggccccgc ccgccgcttc ctccgagccg gcagctccgg cagatgaggc ggccccgccg
     2881 gaaatggtcg aaccgtcg
//
LOCUS       DAOPPA010000421         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37637_length_2882_cov_3.443933, whole genome shotgun sequence.
ACCESSION   DAOPPA010000421 DAOPPA010000000
VERSION     DAOPPA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37637_length_2882_cov_3.443933"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKH54_11455"
     CDS             <1..868
                     /locus_tag="PKH54_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00554.1"
                     /translation="ALAEFLFDDETAMVRIDMSEYMEKHSVARLIGAPPGYVGHDEGG
                     QLTEAVRRRPYTVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGMGRTVDFSNTVLVMTS
                     NVGSEIMAAAAGNGSDGLDGPDGEGGEGRREGGESGEGRGGVNRGVDTTGSLDAGASR
                     PAVDADRVRDQVMAVLRMQFRPEFLNRLDDVILFNSLERADMEKIVDIQMRRILGLLA
                     GRSITLELTPAARALLADKGWDPVYGARPLRRAIERMVLNPLADEILSGRILPGTRVN
                     ADAADDRIVFKV"
     gene            1062..1343
                     /locus_tag="PKH54_11460"
     CDS             1062..1343
                     /locus_tag="PKH54_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00555.1"
                     /translation="MRFVFATMFLLLVSGIVACDEVETYPTCTGNETCTSEVRTDCRG
                     DRIILICDAENFKCGIDCVDACEFGEYDGYAGECKYDPAKGREVCLCLE"
     gene            1534..>2882
                     /locus_tag="PKH54_11465"
     CDS             1534..>2882
                     /locus_tag="PKH54_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00556.1"
                     /translation="MLRVAMRLLAFSMVAFGLVGCGWLAGGDDDQVPDPPPLPSVMGL
                     ESRVIDVIPGETVEIRVTMTDASGKPFEPDSFQVWGQIHRLDGGGGWSADGATLCDSP
                     TVIDVPSEGQRLITFVAGQRGQCMIQARVKTCSGVRGSPCIRELSGEYGPVFVNVMGG
                     DTDSIAPLQPSLTIVPGETRPVWAIPMMAPDKDGLRYPSGQPWKISVRDSAVAVVDGE
                     RRVKGVAPGTTFLDFQSQGVRTEIPVTVVQGSISAPPDGRHRLEVVDFDGTSDTGDIR
                     GVPVHLRNPIFSHKHRLALDSRGWPSAVFEATAVAVQWNALLHMEWTGSGFGVQQIGG
                     FRENLWQPQYAVDSNDVRYVVAQSSVMQGLHVAIQRPGEQPGTWSFMQIPRRHDMDAA
                     DADPLYWTGDHNLDMDRELMSILPRPGGGAWLAYLVWQHDATDPVTPCVRLLRLATMT
                     "
BASE COUNT          525 a          798 c          929 g          630 t
ORIGIN      
        1 ggctctggcc gagttcctgt tcgacgacga gaccgccatg gtacgcatcg acatgtccga
       61 gtacatggaa aagcacagcg tggcgcgtct gatcggcgcg cccccgggtt atgtcggaca
      121 tgacgagggc gggcagctta ccgaggcggt tcggcgacgt ccttataccg tcgtcctgtt
      181 tgacgagatc gagaaggccc atcccgacgt gttcaacgtg ctgctgcagg tgctggacga
      241 cgggcgcctg accgacggca tggggcgcac ggtcgatttt tccaacacgg tcctggtaat
      301 gacgtcaaac gtcggcagcg agattatggc tgcggccgct ggaaacggtt cggacggatt
      361 ggacggtccg gacggcgagg gcggggaagg gcgccgcgaa ggcggcgaaa gcggcgaagg
      421 gcgcggcggt gttaatcgcg gcgttgacac aacgggttcc cttgatgccg gtgcatcgcg
      481 gccggcagtt gacgccgatc gggtgcgcga ccaggtcatg gccgtgctga ggatgcagtt
      541 ccggcctgaa ttcctgaacc gactggacga cgtcatcctg ttcaacagcc tggaaagggc
      601 cgacatggaa aagattgtcg atatccagat gcggcggatc ctgggtctgc tggccggtcg
      661 cagcatcacg ctggaactga ccccggccgc cagggcgctg ctggccgaca agggctggga
      721 tcccgtctat ggcgccaggc ccttgcgccg cgctatcgaa cgcatggtcc tgaacccgct
      781 ggccgacgaa atcctgtcgg gccgcatcct gcctggcacc cgcgtcaacg cagacgccgc
      841 agatgatagg atcgtcttca aagtctgatc ccccttcact tccctgtacg ggcggattac
      901 atccgcccgc tgcgattcag cacattcccc gtacgggcga atgaaaattc gcccgccgcg
      961 attcttcgca tttatcccgg aaaatatccg gaagaatttt ctggacgaag gtcgaaccca
     1021 aagctagagt cacagcgttg gcttataaaa ggggtacctg aatgcgtttt gtatttgcaa
     1081 cgatgttcct gctgcttgtt tccgggattg ttgcctgtga tgaagttgag acgtatccga
     1141 cctgtaccgg taacgagacc tgcacatcgg aagttcggac cgattgcagg ggtgacagaa
     1201 tcatcctgat ttgcgacgcc gagaatttca aatgcggcat tgattgcgtc gatgcctgcg
     1261 agttcggcga atacgatggc tatgccggcg aatgcaagta cgaccccgcc aagggccgtg
     1321 aagtctgcct ttgtcttgag tagccccacc tcccgtcaac agcttcccag ttcggctctg
     1381 tccgtgcttc cggtgatgca gcctgtcgtg acttgccgtt gtgaattttt gcctcaggca
     1441 ttgacttggc gtggttgaaa cgtctatttt ccgccatctg cttttgatcc tggcttgact
     1501 attacagttg agtacatcca aaggagcttt tttatgttgc gcgttgcgat gcggcttctg
     1561 gctttttcga tggtcgcgtt cggactcgtg ggatgcggct ggcttgccgg tggcgatgat
     1621 gatcaggttc ccgatccgcc cccgttgccg tcggtgatgg ggctggaatc acgcgtgatc
     1681 gatgtgatcc cgggtgagac ggttgaaata agggtgacga tgacggacgc ctctggcaag
     1741 ccgtttgaac ctgattcgtt ccaggtttgg ggccagattc acaggcttga tggcggtggc
     1801 ggttggtccg ccgacggcgc aacgttgtgc gattcgccca cggtcatcga tgttccttcg
     1861 gaaggccaga ggttgattac gttcgtggcc ggccaacgcg gtcagtgcat gattcaggct
     1921 cgtgtcaaga cgtgcagcgg tgttcgcgga tccccgtgta tccgtgaact gtccggtgaa
     1981 tacggcccgg tattcgtcaa tgtcatgggt ggcgataccg attcgattgc gccgctgcaa
     2041 ccttcgttga cgatcgtccc gggcgagacc cggcccgtgt gggccatacc gatgatggcg
     2101 cccgacaagg acggcctgcg ttatccgtcc gggcagccgt ggaagatctc ggtcagggac
     2161 tcggcggtcg cggtggtcga tggcgaaaga cgggtcaagg gtgtcgcacc cggcacgacc
     2221 ttcctggatt tccagagcca gggcgttcga accgaaattc cggtgactgt tgttcagggc
     2281 agcatttccg cgccaccgga cgggcgtcat cggctggaag tcgtcgattt cgacggcacc
     2341 agcgacacgg gcgacatccg cggcgtgccg gttcatctga ggaacccgat tttttcccat
     2401 aaacaccgac tggccctgga ttcgcgaggg tggccatcgg cggttttcga ggcgaccgca
     2461 gtggcagtcc agtggaacgc tctgctgcat atggaatgga ctggaagcgg tttcggcgtg
     2521 cagcagatcg gtggtttccg ggaaaatctc tggcagccgc agtatgccgt tgattcgaac
     2581 gatgtcaggt acgtcgtggc acagtcaagc gtcatgcagg ggttgcatgt cgcgattcag
     2641 aggcccggcg aacagcctgg cacctggagt ttcatgcaga tccccaggcg gcatgacatg
     2701 gacgcggcgg atgccgaccc attgtattgg acaggtgatc acaacctgga catggatcgc
     2761 gagctgatgt cgattctgcc gcgtccaggt ggcggggcat ggcttgctta tctggtgtgg
     2821 cagcacgacg cgaccgatcc tgtcacccct tgcgtgcgtc tgttgcgcct tgcgaccatg
     2881 ac
//
LOCUS       DAOPPA010000422         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37645_length_2881_cov_4.298301, whole genome shotgun sequence.
ACCESSION   DAOPPA010000422 DAOPPA010000000
VERSION     DAOPPA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37645_length_2881_cov_4.298301"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKH54_11470"
     CDS             <1..449
                     /locus_tag="PKH54_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00557.1"
                     /translation="PERQEKVAEKQAEKPVEKAKPAPVNTANDANALLAQLHKGKSDD
                     GDKKKGSGTAEGSSGLPDRLTVSQVSRGFGAKKAALDACVRDAGEPLPYKAQAKVEIE
                     GSGRVVSAKLVGGGNAKGCLEGVLRSVTFDRFGGENMKVPYTISVK"
     gene            640..1308
                     /locus_tag="PKH54_11475"
     CDS             640..1308
                     /locus_tag="PKH54_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HOD00558.1"
                     /translation="MKTRVIAIIACAFTVLAAGGVLAAEAAPEAGAGIQVIQQKPFIK
                     AMRFEIEPTFNLPMNETATQHLGVGVQLRFHIKDWVAIGAEYIKYFGWTREDSDGGRD
                     NYMVESRLMDFYVGANVSFVPLTGKFLWLGRYGRPVYWDLYVTAGVGAQKTLYFDYHA
                     SGNIGVGFRLAATQWLTVNFEVRDYMFMENFGPDQDKFMNNAVFMVGLGFFLPFTHKY
                     VHPK"
     gene            1323..2024
                     /locus_tag="PKH54_11480"
     CDS             1323..2024
                     /locus_tag="PKH54_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HOD00559.1"
                     /translation="MKRIAILLAVVLLPVAVSAQEASLADAPSVRHQKLIRGGRHELT
                     PAFGVTINQKYNTDLLFQLSYEYHFYDFFSAGLEIGYAGVGFRTGQGKIIGKMVDEYA
                     QSSQNPTDAKFGRSGLGLLALAKVSFVPLAGKIVLGGKHLGYADIHLTAGVGFGTVSY
                     YDWDNPPGSSSVAAMVGGGVRFFPIELIGLTFDVRDYMVLRKLSSTDKKAELSQNPTF
                     IFGVSFFLPEVKRGR"
     gene            2068..2556
                     /locus_tag="PKH54_11485"
     CDS             2068..2556
                     /locus_tag="PKH54_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOD00560.1"
                     /translation="MKRHFAILVLACAGLVPVSASAGHYGVESIGLFDDQMIQKMVGL
                     GLEDTSRLWSATTTPAKRREVARKLSVRPATVLEWHQFCDLLRVDGVGPKIARLMTHA
                     GVRDLKSLAKQNPSALVPKLKAVRSKVPELGKFPDEGNLQDWVAQAVEMVRKDRNRKP
                     GR"
     gene            2574..>2881
                     /locus_tag="PKH54_11490"
     CDS             2574..>2881
                     /locus_tag="PKH54_11490"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOD00561.1"
                     /translation="MGAPDSILVSGIGTLLPMDGTTDRVESAALIIKDGRVAWRGPAS
                     EIPRELEAGAARFDAGGGLVTPGLIECHTHLLHCGSRAAEYEARARGITYEQIAREGG
                     G"
BASE COUNT          542 a          805 c          921 g          613 t
ORIGIN      
        1 agccggaaag gcaggagaag gtggccgaga agcaggccga gaaaccggtc gagaaggcga
       61 agccggcccc cgtcaatacc gccaacgatg cgaacgccct gcttgcccag cttcacaagg
      121 gcaagtccga cgacggcgac aagaagaagg gttcgggaac tgccgagggg tcttccggcc
      181 ttccggatcg tctgaccgtt tcccaggtca gccgcggttt cggtgcgaag aaggccgctc
      241 ttgacgcctg tgtcagggat gccggcgaac cgctgccata caaggcgcag gccaaggttg
      301 aaatcgaggg atccggccgc gtcgtcagcg cgaagctggt tggcggcggg aacgcgaagg
      361 gctgcctcga gggagtgctt cgttcagtga ccttcgaccg attcggcggc gaaaacatga
      421 aggttccata cacaatctcg gtcaagtgac gtgttccagg cggcggcgtc cgattgaatt
      481 tcgccgcgtt accagcccat ttccatacct ttgatattga cgttgcacat aatgacggtt
      541 aaattgcatg gcatcggacc ttgcggcgtg ccgtgtcctg cggatatcga ttgatgcatc
      601 cgcggcagcg gttggggctt tgtgtccatt gggggcccaa tgaagacaag ggttatcgca
      661 atcattgcat gtgcgttcac cgtcctggct gcgggcggcg ttctggctgc cgaggctgcg
      721 ccggaagccg gtgccggcat ccaggtaatc cagcagaagc cgttcatcaa ggcgatgagg
      781 ttcgaaatcg agcccacctt caatctgccg atgaacgaga ccgcgaccca gcaccttggc
      841 gtcggggttc agcttcgctt ccacatcaag gactgggttg ccatcggcgc cgagtacatc
      901 aagtactttg gctggaccag ggaagattcg gacggcggcc gcgacaatta tatggtcgag
      961 tcgcgcctga tggatttcta tgtcggcgcg aacgtttcgt tcgtcccgct taccggcaag
     1021 ttcctgtggc ttggccggta tggccgtccg gtctattggg acctgtatgt caccgcgggc
     1081 gtcggggccc agaagacact ctatttcgac tatcatgctt cggggaatat cggggtcgga
     1141 ttccgactgg ccgcgactca gtggctgacg gtaaacttcg aggtcaggga ttacatgttc
     1201 atggagaact tcggcccgga ccaggacaag ttcatgaaca acgccgtatt tatggtcggc
     1261 cttggcttct tcctgccgtt tacccataag tacgttcacc cgaagtgatt gggggccgag
     1321 taatgaagcg tattgcaata ctactggcag tcgttcttct cccggtcgcg gtgtccgccc
     1381 aggaggcctc tcttgccgat gcgccctccg tcaggcacca gaagctgatt cgtggcggtc
     1441 gccacgaact tacaccggct ttcggcgtca ccatcaacca gaagtacaac acggatctgc
     1501 ttttccagct ttcgtacgaa taccatttct atgacttctt ctcggccgga ctcgagatcg
     1561 gctacgcagg cgttggtttc cggacgggcc agggaaagat catcgggaag atggtcgatg
     1621 agtacgccca gtccagccag aatccgacgg atgccaagtt cgggcgctcg gggcttggcc
     1681 ttctggcgct ggccaaagta tcgttcgtgc cgcttgcggg aaagatcgtc cttggcggaa
     1741 agcaccttgg ctatgccgat atacacctga ccgccggcgt cggtttcgga acggtttcct
     1801 actacgattg ggacaatcct cccggatcgt cttcggtggc ggccatggtc ggcggtggcg
     1861 tccggttctt cccgatcgag cttatcgggc tgacgttcga cgtccgcgac tacatggttc
     1921 tgcgcaagct ttcgtcaacg gacaagaagg ccgaactgtc ccagaacccc actttcattt
     1981 tcggggtgtc cttcttcctt cccgaagtca agcgcggtcg gtgacggtcg ggattttccg
     2041 tttccccgtt caagaggttt gaatttcatg aaaaggcatt tcgccatcct ggtgctcgcc
     2101 tgtgcgggcc ttgttccggt ttcggcctcg gccgggcact acggggtcga gtcgatcggc
     2161 cttttcgacg atcagatgat ccagaagatg gtcggcctgg ggcttgaaga tacttcaagg
     2221 ttgtggtccg ccaccacgac ccccgcgaag aggcgagagg tggcccgcaa gttgtcggtg
     2281 cggcccgcga cggtcctgga gtggcatcag ttctgcgacc ttctcagggt tgacggggtt
     2341 ggcccgaaga ttgcgcggct gatgacgcat gccggggttc gagacttgaa gtcccttgca
     2401 aagcagaatc cgtctgccct tgtaccaaag ctgaaggcgg ttcggtcaaa ggttcccgag
     2461 cttggtaagt ttcccgacga gggcaatctc caggactggg tcgcccaggc agttgaaatg
     2521 gtcaggaagg acaggaaccg caagccgggc cgttagtcgg caggtggtgt tcagtgggcg
     2581 cgccagattc aattcttgta agcggcatcg gaacgttgct gccgatggac ggcaccaccg
     2641 acagggtgga gtcggcggcg ctgatcatca aagatggcag ggtcgcgtgg cgtgggccgg
     2701 cttcggagat ccctcgggag ctcgaggccg gggctgccag gttcgatgcg ggcggcgggc
     2761 ttgtgactcc cggcctgatt gagtgtcaca cgcatcttct gcattgcggc tcacgcgccg
     2821 cggaatacga ggcaagggcg cggggcatca cctatgaaca gatcgcccgc gagggcggcg
     2881 g
//
LOCUS       DAOPPA010000423         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37841_length_2869_cov_2.908316, whole genome shotgun sequence.
ACCESSION   DAOPPA010000423 DAOPPA010000000
VERSION     DAOPPA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37841_length_2869_cov_2.908316"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKH54_11495"
     CDS             <1..396
                     /locus_tag="PKH54_11495"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOD00562.1"
                     /translation="PRDIPDFLNMLTAERRVRSRDRKGQTRIEWKKVSEHRRNEAWDC
                     MVYALAAAHSLVIAGLRMDDALAVRPRPTPVKPPRPEPDPSPVEPAAARSDYRFQRNR
                     VEPPSRPVRVNYWDRRSGYFNPRIDDDDD"
     gene            complement(404..1060)
                     /locus_tag="PKH54_11500"
     CDS             complement(404..1060)
                     /locus_tag="PKH54_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00563.1"
                     /translation="MSNSKKVTETKGKNVGIRGKTIVASETTTAEPIKPDATTDPIAI
                     DNDAPAPATDTVETTPTPDETTTPEPTTERPPRRDPRLPEVGTPIVRKWRGHSVVVKE
                     MDDGVFKVKVDSAYIGEARSLTKAAEMALATEGITSGVNGFAWFHVGEEAKSKHAATD
                     RRLPALTAMVARIEERIQAAEDRANKLRIDRDEAIKQIEKIRAAMEAAAAAEATASED
                     "
     gene            complement(1118..1345)
                     /locus_tag="PKH54_11505"
     CDS             complement(1118..1345)
                     /locus_tag="PKH54_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00564.1"
                     /translation="MNAQEAKTFDHQVQVWNHKGSHRTRWYACQVIGRHPAPHDFAAQ
                     GFPDDWYDVRLRDETVITWCNPECVRVKKAN"
     gene            1702..>2869
                     /locus_tag="PKH54_11510"
     CDS             1702..>2869
                     /locus_tag="PKH54_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOD00565.1"
                     /translation="MSLNPQVTMKPVAELNPYQNNPRTHSRDQIAKVVRSIREFGWTN
                     PILLDRDGMVVAGHARLEAAKSIGLTEVPTICLSHLSPAQVRAYVIADNQLALDSSWD
                     EELLRLELGALDGMNFDLSLTGFDSADIDRMLAELDVINGNEGLCDEDAVPEPPAEPT
                     TKPGDLWILGDHRLLCGDSSKATDLDRLLGGASIHLVNMDPPYNVKVEPRSNNAIAAG
                     LSSFTAQKTTHHQALDLARHPEKAHGTTSVMRAKDRPLLNDFVTAEAFDEMLMAWFGN
                     AARVLEPGRGFYIWGGYANCSNYPPAMKACGLYFSQSIIWDKEHPVLTRKDFMGAHEW
                     CFYGWKEGAAHQFLGPNNATDLWHVKKVNPQSMVHLTEKPVELARRSIEYSSRRG"
BASE COUNT          511 a          834 c          873 g          651 t
ORIGIN      
        1 ccgagggaca tcccggattt cctgaacatg cttactgccg agcggcgggt cagaagccgc
       61 gaccgcaagg gccagactcg tatcgaatgg aagaaggtat ccgagcatcg ccgtaacgaa
      121 gcgtgggatt gcatggtgta tgcactcgca gccgcgcatt cgttggtgat cgctgggctg
      181 cggatggacg atgccctcgc tgtacgccca cggccgaccc ctgtgaagcc ccctcgacct
      241 gaacctgacc cctcaccggt cgagcctgca gccgccagga gcgattacag attccagcgc
      301 aaccgcgtcg agccaccgtc gcgtcccgtg cgggtgaact actgggaccg gcgtagtggc
      361 tacttcaacc cgaggattga cgatgatgac gattgattac gggtcagtct tcggacgcgg
      421 tggcttcggc ggcagcagcg gcttccatcg cggcgcggat cttctcgatc tgtttgatgg
      481 cttcgtcccg gtcgatgcgc agtttgttgg cgcggtcctc ggcggcctgg atgcgttctt
      541 cgattcttgc gaccatcgcg gtcagcgccg gaagcctgcg gtcggtggcg gcgtgcttcg
      601 acttggcttc ctcaccgacg tggaaccatg caaaaccgtt gaccccggac gtgatgcctt
      661 cggtggcaag cgccatctcg gcggccttgg tcaggctgcg ggcttctccg atgtacgcgc
      721 tgtcgacctt gaccttgaag accccgtcat ccatttcctt gacgacgacg ctgtggccac
      781 gccacttgcg gacgatgggc gttccgactt cgggcaggcg cgggtcgcgg cggggagggc
      841 gctcggtcgt gggttccggc gtggtggttt catccggcgt gggcgtggtt tcgaccgtgt
      901 cagtggctgg agcgggtgca tcgttgtcga ttgcgatcgg atcggtggtg gcatcgggtt
      961 tgattggctc agcagtggtg gtttcgctgg caacgattgt cttgcccctg atgccgacgt
     1021 tctttccctt ggtctcggta accttcttcg aattgctcat atggatctcc ttgcgtgcgg
     1081 tgccgtttcg ggaccatccc gccacggcag tcccgcgtta attagccttc ttgacccgga
     1141 cgcattccgg gttgcaccag gtgatgaccg tttcgtccct caggcggacg tcgtaccagt
     1201 catcggggaa tccttgggct gcgaagtcat gcggggcagg gtgcctgccg ataacctggc
     1261 atgcatacca gcgggtgcga tgcgagcctt tgtggttcca tacctggact tggtggtcga
     1321 acgtttttgc ttcctgcgcg ttcatcgaaa tctccttggt tttcaaggtg ttttccctca
     1381 acccaaggac atgtttgctc tgctttgcga cgaagtgaag tcaaaagact gcttatattt
     1441 caacaagttg catgaatttg atttgcgcac gtttgcgcgg ttttgcgcgt cttgttgcaa
     1501 atcgccgacc gcgacagttc gcgcacgtgg cgtttttcga aggcaccgcg taggttttgc
     1561 catccccgca aacgaaacgg cccaaatcgc gcgtgtaact tcacggaatc acgaacgaat
     1621 cgcgggacga tttcgttcct gttgtgctgt aagtctttgg ccgcagaagc ctttgatttc
     1681 tttcagtaat ggaggtgtca gatgtcgttg aatcctcagg tcacaatgaa gcccgtcgca
     1741 gaactgaatc cttatcagaa caaccccagg acgcattcca gggaccagat cgcaaaggtc
     1801 gtgcgttcca tcagggaatt cgggtggaca aacccgatcc ttctggaccg tgacggcatg
     1861 gtggttgccg gccatgcccg tctggaagct gccaagtcca tcggattgac cgaagtcccg
     1921 accatctgcc tgtcgcatct gtcgccggcg caagtccggg cgtacgttat cgcggacaac
     1981 cagcttgctc tggactcgtc ctgggacgag gaacttctgc gcctggaact cggtgcgctt
     2041 gacggcatga acttcgacct gtcgctgaca ggcttcgatt ctgctgacat cgaccgcatg
     2101 cttgctgaac tcgacgtcat caacggcaac gaaggtctgt gcgacgaaga cgcggtgccc
     2161 gaaccgcctg ccgagccgac cacgaagccg ggcgacctgt ggattctggg cgaccatcgt
     2221 ctgctgtgtg gtgattctag caaggctacg gacctcgacc gtctgcttgg cggagcatct
     2281 atccatctcg tcaacatgga cccgccctat aacgtcaagg tcgagccccg cagcaacaac
     2341 gctattgccg caggtctgtc gtcatttacg gcgcagaaga ccacgcatca ccaggcgttg
     2401 gacctggccc ggcatcctga aaaggcacac ggcacgacgt ccgtgatgcg ggcgaaggac
     2461 cggccgttgc tgaacgattt tgttacagca gaggcgtttg acgagatgct gatggcgtgg
     2521 tttgggaacg ctgcccgtgt cctggaaccc ggtcgcggct tctatatctg gggtggttac
     2581 gcgaactgtt cgaactaccc gccggccatg aaggcctgcg gtctgtactt ttcgcagtca
     2641 atcatctggg acaaggaaca cccggtcctg acgcgtaagg atttcatggg cgcgcacgag
     2701 tggtgtttct acgggtggaa ggaaggcgcc gcccatcagt ttcttggccc gaacaacgcc
     2761 actgacctgt ggcacgtcaa gaaggttaat ccgcagtcga tggtccacct gaccgaaaag
     2821 cccgtcgaac tggcccgtcg ctcaatcgag tattcctccc gtcgtggcg
//
LOCUS       DAOPPA010000424         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_37880_length_2867_cov_2.669986, whole genome shotgun sequence.
ACCESSION   DAOPPA010000424 DAOPPA010000000
VERSION     DAOPPA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37880_length_2867_cov_2.669986"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..>2867
                     /locus_tag="PKH54_11515"
     CDS             352..>2867
                     /locus_tag="PKH54_11515"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD00566.1"
                     /translation="MKTAVISVLFGAAIPFLFMVVPASALAQEFQPPTSGFQPAATAF
                     PYGAALAGKRIVISPGHGWEGDSSGGFQRGNVKFQNCGTCEGITEDIYNAEFCTDHLV
                     PMLRQAGARVYVVRQVDRQRASVTVDDGDAGYSEVAPWGTGTTGGLGWQGDYRTLNGN
                     IAGEATWTLQVPVEGDYWVQMRWAAGTNRCTDARVRVSHAGGVAEFQVNQQIDGSMWV
                     PLARLHFQAGPATVTLDHPPAVGCYIVADAVRLGGGVDSYSGKSWWQVSALDWLAETG
                     PGAIASGYNDVTVRPALANYLDADLFISFHGNANENASARGTSTYRYNCDSGVQWDEL
                     DPGVCDDPSGSAALQYSVQEQVMLDLWSYWDDAFQDDGPLVGNFGELRVLDTVPGFMV
                     ESAFFSNTSPAGGQLFSDNQSMHDPRFRRIVARGILQGIVSELNPAVPIPPEPPTHLS
                     LTNDGGGALVAKWRTAAGAAGYRVYKAVGGRGFDGGTIVTGNSLKLTGLASGAVVAVR
                     VTSLNAGGESTPSPAVAASPGKADILVVNAFDRLDAWVREDHNHRDYVIEHGLALAQA
                     GLAFDGALDEAAIDGDVFIEEYGMLDWALGRESTAAVTINDYGQGELRAYQELGGCIL
                     VSGTEIAWDLGNMGTAAEVEFLAQVLGAEFAADDAISFVVQAEPEGPWADMGSFGFDD
                     GSGGTYRASYPDVLLPAAGAQVLLRYGTGGAAAVGLVAGEGRAVTMGFPIETINSLAV
                     RADLFERIVDFCGLGENGTADDSGEQAADEALADVGPGDNGGFDQGGGGDQGTSADVW
                     DYPDLHKSDTVDAGGGTDLSENDAGQGDDVADAGADGGSD"
BASE COUNT          518 a          787 c          968 g          594 t
ORIGIN      
        1 cggcggcgtc caggttcctg tcttcggtgg cgtcgccagg cacatcattg ttgtgcagat
       61 ccgggaagcc gctatccggc gccccgttgt ccgtgaaact gtcttcaatc gtgtccgccg
      121 tcacatcgtc agtcacgtca atccccgtgt cctcgccgcc ttccgaaccg gagcacgcgg
      181 caaacgcgca gcagcgctaa cgtcgtgcag gtcaacagtg tcattcttgt cgcaggtgtc
      241 atcactggca gtttcccccc aaaggcataa gttgacattt gacaatagaa gtttttgaaa
      301 tgcaacgcac ttccgtgggt tagaataaat cggtcgtttc ggggagatcc gatgaagact
      361 gccgtcatct ccgttctgtt tggtgccgcc ataccgtttc tgtttatggt tgttcccgcg
      421 tcggcacttg cccaggaatt ccagcccccc acatccgggt tccagcctgc tgccacagct
      481 tttccatacg gggcggctct ggcaggcaag cggatcgtga taagtccggg acatggatgg
      541 gaaggtgact ccagcggggg cttccagcgt ggaaacgtca agtttcagaa ttgcgggaca
      601 tgcgaaggca tcaccgagga catctataac gcggaatttt gtaccgacca cctcgtgccg
      661 atgcttcgcc aggccggggc gcgcgtctat gtcgtgcgcc aggtcgaccg gcagcgtgct
      721 tccgttacgg tcgatgacgg ggatgccggg tattcagagg tggctccatg gggcactggc
      781 acgaccggcg gccttgggtg gcagggcgat tatcgtacct tgaacgggaa catcgccggc
      841 gaggccacat ggacgctgca ggttcctgtc gagggcgatt actgggttca aatgcgctgg
      901 gccgccggaa ccaaccgatg cacggacgcc agggtacgtg tctcccacgc cgggggtgtt
      961 gccgaattcc aggttaacca gcagattgac ggcagcatgt gggttcctct ggcaaggttg
     1021 catttccagg cgggaccggc aaccgtgacg ctggatcatc ccccggcggt cggctgttac
     1081 atcgttgccg acgccgtgcg gctgggcggc ggggtcgatt cgtattcggg caagtcctgg
     1141 tggcaggttt cagcgcttga ctggctggcc gagaccgggc cgggggccat cgcaagcgga
     1201 tacaacgacg tgaccgtaag gccggcgctg gccaattacc tggacgccga cctttttatt
     1261 tcctttcatg gaaatgcgaa cgaaaacgcg tcggcacgtg gcacatcgac atacaggtac
     1321 aactgtgaca gcggggttca gtgggacgaa ctggatcctg gcgtctgtga tgatccttcg
     1381 ggcagcgccg ccctgcagta ttctgtccag gaacaggtga tgctggattt gtggtcgtac
     1441 tgggatgatg catttcagga tgacggaccg ctggtgggca atttcgggga attgagggtt
     1501 cttgatacgg ttccggggtt catggttgaa tcggctttct tttcaaatac ttcgccggct
     1561 ggcggccagc ttttttccga caaccagtcg atgcatgacc cgcgtttcag gcggatcgtc
     1621 gcacgtggca tccttcaggg cattgtcagc gagcttaatc cagcggtgcc gataccgccg
     1681 gagccaccca cccatttgtc actgacgaac gacggcggcg gcgccctggt tgcgaagtgg
     1741 cgaaccgccg cgggggcagc cggttatcgg gtttacaagg ccgtgggcgg tcggggcttc
     1801 gacggcggaa cgatcgtcac cggcaacagc ctgaaactga ccgggcttgc gtcgggtgcg
     1861 gttgtcgcgg ttcgcgtgac aagcctgaac gccggtggcg agtcaacccc gtcgcctgcc
     1921 gtggcggcaa gcccaggaaa ggccgatatc ctggtcgtca atgcgtttga caggctggat
     1981 gcctgggtac gggaagacca caatcaccgg gattatgtga ttgaacacgg cctggcgctg
     2041 gctcaggccg gccttgcctt tgacggcgca ctggacgagg cggcgatcga tggcgacgtc
     2101 ttcattgaag aatacgggat gcttgactgg gcgctcgggc gggaatcgac cgccgccgtg
     2161 acgatcaacg attatggaca gggggaactg cgcgcatatc aggaactggg tggttgtatc
     2221 ctggtttcgg gaaccgagat agcctgggat cttggtaaca tgggaaccgc tgccgaggtc
     2281 gagtttcttg cccaggttct tggggccgaa ttcgcggccg atgatgccat cagcttcgtt
     2341 gttcaggccg aacctgaagg tccctgggcc gacatggggt cgttcggctt tgatgacgga
     2401 agcggcggca cctatcgcgc cagttatccg gatgtcctgc tgccagccgc gggtgcgcag
     2461 gtgctgctca ggtacggaac cgggggggcc gcggccgtcg gtctggttgc cggcgagggg
     2521 agggctgtga cgatgggctt cccgatcgaa accatcaaca gccttgccgt ccgcgctgac
     2581 ttgtttgaaa ggattgtcga tttctgcggg cttggcgaaa acgggacagc cgacgattcc
     2641 ggggaacagg cggctgacga ggcacttgcg gatgtcgggc ccggcgacaa cggcgggttc
     2701 gatcaaggcg gcggtggcga tcagggcacg tccgcggacg tgtgggatta tccagacctg
     2761 cataagtcgg acacggttga tgccgggggc ggcacggact tgtcagaaaa cgatgcgggg
     2821 cagggcgatg acgttgccga tgcgggagcc gatggtgggt cggacag
//
LOCUS       DAOPPA010000425         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38036_length_2857_cov_4.164168, whole genome shotgun sequence.
ACCESSION   DAOPPA010000425 DAOPPA010000000
VERSION     DAOPPA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38036_length_2857_cov_4.164168"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKH54_11520"
     CDS             <1..449
                     /locus_tag="PKH54_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD00567.1"
                     /translation="LDEIQAAILLAKLDHLDTWTSERQMNAATYERLLRPAEEAGMLR
                     LPRAASGSRHVWNQYTLTIPDRRQEVIDRLDAAGIGWAIYYPLCLHLQECFVDLGGRH
                     GDCPQAELTSATALSIPIFPGLSRSQLRYVADAVIGEQSRIDIDAG"
     gene            complement(782..1180)
                     /gene="rpsI"
                     /locus_tag="PKH54_11525"
     CDS             complement(782..1180)
                     /gene="rpsI"
                     /locus_tag="PKH54_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD00568.1"
                     /translation="MAEANVMWATGRRKEAAARVRVLPGTGKITVNGRDLDNYFCRPN
                     HRILVRTPFVVLGNGDQFDVLCTVRGGGETGQAGAIRHGIARALAAMNVEEWRGPVKR
                     AGFLTRDPRAKERKKYGRPGARKRFQYSKR"
     gene            complement(1191..1628)
                     /gene="rplM"
                     /locus_tag="PKH54_11530"
     CDS             complement(1191..1628)
                     /gene="rplM"
                     /locus_tag="PKH54_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD00569.1"
                     /translation="MRTRSFATKDVDRKWYVVDVDGVTLGRAAARIAMILRGKHKPTY
                     TPHDDTGDFVVVVNSAKAVLTGRKLEQKMFVWHTGYMGHLRERTYADVMENKPSEVFK
                     RAVKGMLPRGVLGRQMLTKLKVYEGAEHPHEAQQPEKLDISKV"
     gene            complement(1743..>2857)
                     /locus_tag="PKH54_11535"
     CDS             complement(1743..>2857)
                     /locus_tag="PKH54_11535"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOD00570.1"
                     /translation="MTTPEYFGEYAKRYISAGVGLFGGCCGTTPEHVRQIAGEIRMVR
                     GGRIEIPADAQSGGTCQVNGIVPPPVGDRSGFGRDLAAGFVVSVELDPPTGLDTSRAL
                     AGAALLKSAGVKYVNIADGPRATVRMSALAMAHALQDSGLEPVLHVTCRDRNLLGLQA
                     DLLGAWSMGLRNLLVITGDPSKLGDYPDATTVYDLDSVGLLRLVGNLNRGVEPSGRRM
                     ASGTAFVKGCGAEPGAVDPDREIARLASKVEAGAEYVMTQPVFDHEVMNRFLERVRRE
                     IGEKFPVVMGILPLASFRNAEFLHNEVPGMRIPDEIRARMKQAGFADAARAEGVAIAC
                     EMAGAFRDRIGGIYIMPPFNRFDVAVAVLQGLGLAP"
BASE COUNT          580 a          914 c          821 g          542 t
ORIGIN      
        1 ggcttgatga gattcaggca gcgatacttc tggccaagct ggatcatctg gacacttgga
       61 catccgaacg ccagatgaac gccgccacct atgaacgcct gctgcgtccg gccgaggaag
      121 ccgggatgct ccggctgccg cgagccgctt ccggcagccg ccatgtgtgg aaccaataca
      181 cgctgacgat tccggacagg cgccaggaag taatcgaccg gcttgacgcc gccggcatcg
      241 gctgggcgat ctactacccg ctctgcctgc atcttcagga atgcttcgtc gaccttggcg
      301 gcaggcacgg cgactgtccc caggccgaac ttacgtcggc gacggccctg tcgatcccaa
      361 tcttccccgg actctcacga tcacaactga gatatgtggc ggacgccgtg atcggcgaac
      421 aaagtcggat tgacatcgac gccggttagt ttcatagtta tacggcgact gaatctcctc
      481 cccccggaga tgccgtcgca tcatccatca ggaaggcaat gccttcccga cgaccccgcg
      541 atggccgggg agtgtcaatg atggttcgcc cgattcggca cccccctgcc ggcaggtgaa
      601 tcgacactcc ccggccatca aactttccgg gccatcgcag tccgcaccat acagtcacgg
      661 cacaaggcga atgacgccaa ctgcacgatt acacggggag aatgcggata cacaggggga
      721 tcgaaaatcc aatacagaaa tggtgttgaa aaaagttcga ctggcggctg gtgtttccgg
      781 actagcgctt ggagtactgg aagcgcttgc gggcacctgg acgaccgtac ttcttacgtt
      841 ccttcgcgcg cgggtcgcgg gtcaggaaac cggcccgctt gaccggtcca cgccactctt
      901 caacgttcat cgcggcaaga gcgcgggcga tgccgtggcg gattgcgccg gcctggccgg
      961 tttcaccgcc accgcgaacg gtgcacagga catcgaactg atcgccgttg ccaagcacca
     1021 cgaacggggt gcggaccagg atgcgatggt tcggacggca gaaataattg tcaaggtcgc
     1081 ggccgttgac cgtgatcttc ccggtaccgg gcagaacacg aacccgagcg gcagcttcct
     1141 tgcgacgacc agtggcccac atgacgttgg cttcagccat tttgacctct tcagaccttc
     1201 gaaatgtcga gtttttcggg ctgctgagct tcatgaggat gctcggcgcc ttcatatacc
     1261 ttcagcttgg tcagcatctg gcgaccaaga acgccgcggg gaagcatccc cttgacggcc
     1321 ctcttgaaca cttccgacgg cttgttttcc atgacgtcgg cataggtgcg ttcgcgcagg
     1381 tgacccatgt aaccggtgtg ccagacgaac atcttctgtt caagcttgcg accggtaaga
     1441 accgccttgg ccgaattgac cacgacgacg aaatcaccgg tgtcgtcatg gggggtgtag
     1501 gtcggcttat gctttcccct gagaatcata gctatcctgg cggcagccct tccaagggtc
     1561 acgccatcga catcgaccac ataccatttg cggtcaacat cctttgtcgc aaaacttctg
     1621 gtccgcattg tgtcctcgcc ttccaaggga cagggcgaat cttttacccg aaacagaaac
     1681 caaagtcaag agatacgtcc cttgattttg atggttcctt gcagcagcgc gaatacgaac
     1741 cttcacggag ccagcccaag gccctgaaga accgccacgg cgacgtcgaa acggttgaac
     1801 gggggcatga tgtagatgcc gccaatcctg tccctgaaag caccggccat ctcacaggct
     1861 atggcgacgc cttccgccct tgcggcatcc gcgaagccgg cctgcttcat cctggcgcgg
     1921 atttcgtcgg ggatgcgcat cccgggaact tcattgtgca ggaactccgc gttccggaag
     1981 gacgcaagag gaaggattcc catcacgaca gggaacttct caccaatctc gcgacgcacg
     2041 cgttcaagga aacgattcat cacttcatga tcgaacaccg gctgcgtcat gacgtattcc
     2101 gcgccggctt cgaccttgct tgccagccgc gcgatctcgc gatccggatc gaccgcgccc
     2161 ggctccgcgc cacagccctt gacgaaggcg gtacccgaag ccatgcgccg cccggaaggc
     2221 tcgacgcccc ggttcaggtt gccgaccagc cgcagcagtc ccaccgaatc caggtcataa
     2281 acggtggtgg catcgggata gtcgcccagt ttcgacggat cgccggtgat caccagcagg
     2341 ttcctcaggc ccatcgacca ggcgccgagc agatccgcct gaagccccag caggttccga
     2401 tccctgcatg tgacgtgcag aaccggctcc aggccggagt cctgcagtgc gtgggccatc
     2461 gccagggccg acatccttac ggtggccctg ggaccgtcgg cgatattgac gtatttcact
     2521 cccgcgctct tcagaagggc ggctccggcc agcgcgcggg aagtatccag gcctgtcggg
     2581 ggatcaagtt caacgctgac gacgaaaccg gcggcaagat cgcgtccgaa cccggatcgg
     2641 tcgccgaccg ggggcggcac aatcccgttt acctgacacg tgccgccgga ttgcgcatcg
     2701 gccggaattt cgatccgccc tccgcgcacc atgcggattt caccggcaat ctgccttacg
     2761 tgttcaggcg tcgtgccgca gcatcctcca aaaagaccca caccggccga gatgtatcgt
     2821 ttcgcgtact cgccgaagta ttccggggtc gtcatgt
//
LOCUS       DAOPPA010000426         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38051_length_2856_cov_3.958229, whole genome shotgun sequence.
ACCESSION   DAOPPA010000426 DAOPPA010000000
VERSION     DAOPPA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38051_length_2856_cov_3.958229"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKH54_11540"
     CDS             <1..1025
                     /locus_tag="PKH54_11540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD00571.1"
                     /translation="PDAADITSLDDSIDELPDARSDEGELPQGDAVAPDSGVEPEVDP
                     VLANTLAQIMQEHINFSSDPGMVLAVRTPEGAWWSGAVGMADLVDEVALQPDSQFRVG
                     SNTKPIVAVVILQLVEEGKIDLDQPLTTYLPEYPEWSDITVRMLLNMRSGLNEFLNVE
                     LCLLSVLLEPTRAFTPAEIVGFVMDSENNGRLFEPDTDGYYSNTNYVLLGMIIEAVSD
                     KTAEQEIQERILDPLGMTRTYLDDGTGDDSELVDGYLDIVAGGVVVGVSPSQAAMIPN
                     VQYIDGLVIGTHLMHASVTWTSGALVSTSYDMGRFVHALMTGRLLKPATMSRCRTRIP
                     RRCLAG"
     gene            986..1870
                     /locus_tag="PKH54_11545"
     CDS             986..1870
                     /locus_tag="PKH54_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00572.1"
                     /translation="MSDTHPAALLGGLTEYGLGLQNIPSPEGETWGHGGLNFGYQTQT
                     WYLPEHDITISHIHNFLLNWYDKLQNDLVAAVKAGGYPDFKACGAPDGFFDVPSTNTW
                     FKWAFKGPVTADLPSERIDGNGFFRVEHDGSIEPVVGMYPQVVRTERAGKPILRLTAY
                     GAPVEGDANLTMLTLELSENLFSGLDAQGTKVIGQDNAEEMFGALTELYWAPGTKTVN
                     KFCYTGFSNVLIDGHAFLCEGNRDGMPQVGDTLRVMGNVPVSSDPDYVDYVVADVMKS
                     ARCMCLDGTDWIPCATPN"
     gene            complement(2092..2466)
                     /locus_tag="PKH54_11550"
     CDS             complement(2092..2466)
                     /locus_tag="PKH54_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOD00573.1"
                     /translation="MERANPCIGCGACCAYYRVSFYWGEIDEVVPGGFPSEYVEKLND
                     FRAVIKGTNQPEPRCAALAGEIGKTAHCSIHCVRSSACREFEASYVNGLPNERCDQAR
                     AKYGLEPLTPESWRPHGVPTAA"
BASE COUNT          598 a          799 c          854 g          605 t
ORIGIN      
        1 atcccgatgc cgcggacatc acgtcccttg acgattccat cgatgaactg cccgacgcgc
       61 gaagcgatga aggcgaactg ccgcaaggtg atgcggtcgc cccggattcg ggagtggaac
      121 ccgaggtcga tccggttctt gcaaacacgc ttgcacagat catgcaggaa cacatcaatt
      181 tcagcagcga tcccggcatg gttctggccg tccgaacgcc ggaaggcgcc tggtggtccg
      241 gcgccgtcgg aatggccgat cttgtcgatg aagtcgcgct gcagcctgac agccagttcc
      301 gcgtcggcag caacacaaag ccgattgtcg cggtcgtgat tctgcaactg gtcgaggaag
      361 gaaagattga cctggatcag cctttgacca cctatctacc cgagtatccg gagtggtccg
      421 acataacggt tcgcatgctt ctgaacatgc gcagtggcct gaacgagttc ctgaatgtcg
      481 aactttgcct gctttcagtg cttcttgaac cgacacgggc attcacgccg gctgaaatcg
      541 tcgggttcgt gatggattcc gaaaacaacg gtcgcctttt cgagccggat accgacggct
      601 attattcgaa caccaattat gtgctgcttg gcatgatcat cgaggccgtc tcggacaaga
      661 ccgccgagca ggagatccag gaaaggattc ttgatccact tgggatgaca agaacgtacc
      721 tggatgacgg caccggtgac gattccgaac tggtggacgg gtatctggac atcgtcgcgg
      781 gcggcgtcgt ggtcggggtc agtccgtcac aggcggcgat gattcccaac gtgcagtaca
      841 tcgatgggct tgtgattgga acccatctga tgcatgcgtc ggtcacatgg acgtcgggtg
      901 ccctggtgtc gacgtcatac gacatgggca ggttcgtcca cgcattgatg accgggcgtc
      961 ttctgaagcc ggcaacgatg agcagatgtc ggacacgcat cccgcggcgc tgcttggcgg
     1021 gctgaccgag tacgggctgg gcctgcagaa cataccaagc cccgaaggag aaacctgggg
     1081 ccatggcggg ctgaatttcg gataccagac gcagacatgg tacctgccgg aacatgacat
     1141 cacgatatcg cacattcaca acttccttct gaactggtac gacaagttgc agaacgacct
     1201 tgtggcagcc gtcaaggcag gcggatatcc cgatttcaag gcctgcggcg cgccggacgg
     1261 gttctttgac gtgccctcga cgaacacctg gttcaagtgg gccttcaagg ggccggtcac
     1321 cgccgatctt ccgtcggaac gtatcgatgg caacggcttc ttcagggttg aacacgatgg
     1381 ttccatcgag cccgtcgtcg gcatgtatcc ccaggtcgtc agaaccgagc gtgccggcaa
     1441 gcccatcctg cggctgaccg cctacggcgc gcccgttgaa ggcgatgcca accttacgat
     1501 gctgacgctg gaactttccg aaaacctctt ttccgggctt gatgcccagg gaacaaaggt
     1561 gatcggccag gacaacgccg aggagatgtt cggcgcgctg accgaacttt actgggcacc
     1621 gggaaccaaa accgtcaaca aattctgtta taccgggttt tcgaatgtcc tgattgacgg
     1681 tcacgcattc ctttgcgagg gaaatcggga tggcatgccc caggttggag atacgttgcg
     1741 cgtgatgggc aatgttccgg tttcaagcga tccggattat gtggactatg ttgttgccga
     1801 cgtcatgaaa tccgccaggt gcatgtgcct ggacggcacc gactggatcc cttgcgccac
     1861 tcccaactga aacttccctt ttcccaatca gcgcgaattt tccctgttcc ccgtaagggc
     1921 gaatgaaaat tcgcccgcca gggcaggaaa ttcgtccgct gaaaggtggc caaggtggat
     1981 cgtccggctc cttgtacggg cggatgtaat ccgcccgtac aagggcaggg ggcacattgc
     2041 tcgaccgcag cgggcgaatg aaaattcgcc cggggcacgg tgcaggcatg gtcatgcggc
     2101 ggtcggcaca ccgtgcggtc gccaggattc gggcgtcagc ggttcaagtc cgtactttgc
     2161 gcgggcctgg tcgcagcgtt cgttcggcag cccgttgacg taactggcct cgaattcccg
     2221 gcatgccgat gaccggacgc agtgaatgct gcaatgggcc gtcttgccga tttcgcccgc
     2281 aagtgccgcg catctgggct cgggctggtt cgtacccttg atgaccgcgc ggaaatcgtt
     2341 aagcttttca acgtattcgg acggaaagcc accgggaacg acctcatcga tttcgcccca
     2401 gtaaaaggaa acacgataat aagcacaaca tgcaccacac ccgatacaag ggttcgcccg
     2461 ttccatgatc actgtcgcat ttcaggtgat gattggtcac gacgaacccg gacgggcccg
     2521 caattgggcg cggattgtaa agacggatcg catcttgtgc aagcgatttg taaagtttca
     2581 aattcatttc gcaccggatc ctatggaact gctcacaatc tggggttata ttcccgcaag
     2641 tgcttctgtt tggaaccaga tgaaaccgta tcgaagtcaa agggggattc gatgaatttg
     2701 cgttctgtgt tcatgtccgt ggctgtcgtt gcggcgctga tggtgtttgt cgcgtgcgga
     2761 tccgggacgg agcctgaaaa cgatcagggc actgacgtcg gtctcgatgc ggccggcgag
     2821 ctccctcctg actcggttgt cgttccggac atgacc
//
LOCUS       DAOPPA010000427         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38057_length_2856_cov_2.593717, whole genome shotgun sequence.
ACCESSION   DAOPPA010000427 DAOPPA010000000
VERSION     DAOPPA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38057_length_2856_cov_2.593717"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKH54_11555"
     CDS             complement(<1..184)
                     /locus_tag="PKH54_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00574.1"
                     /translation="MRQFGAADEHREDVAAGDGGSGGICRAGGAGRAGRADGAGGAGG
                     AGGAGRTNLAGCSGGAA"
     gene            330..524
                     /locus_tag="PKH54_11560"
     CDS             330..524
                     /locus_tag="PKH54_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00575.1"
                     /translation="MSIQNETVALNVKPFATDLEYLTAEFAWIQARTRRIEASQLAMN
                     QAPALAKMQSAGMWPAITGD"
     gene            complement(511..1476)
                     /locus_tag="PKH54_11565"
     CDS             complement(511..1476)
                     /locus_tag="PKH54_11565"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD00576.1"
                     /translation="MNFIHQDPEFPQLVQIVARSTGIAAALVEKDYWVTHTLWAIHRT
                     GLDIWFKGGTSLSKGFGLIQRFSEDLDLMVERGTVDALPDITNWTSTNKGPVDKRRAF
                     YDALAATLVVPDVKVERDTTRIDRQARSADYLARYPGALLAELSQTMSPFVRLEVGHA
                     RVVPCVRRPLDSFVHAQLVQLNLLDEYEDNRPLDVRCVHPMVTLFEKLDAMARRYSRD
                     DMEPDTFVRHYEDAAQIIRAEDSLPDMGMTTMALVLDMLNRKDIAEFPRQDEPALVLT
                     DSDKATAIARAYARIAPMFWGPRIPLGEACDTIRTWLGKLNPNHQ"
     gene            complement(1476..2060)
                     /locus_tag="PKH54_11570"
     CDS             complement(1476..2060)
                     /locus_tag="PKH54_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00577.1"
                     /translation="MSLAAQPKLEPGKTYRTRELRCFGENPTRLARRLVREGKLQQAA
                     HGLFYAPLDSRFGQAPPSDDELLRGFLGSDTFVITGPPKWNALGLGTTAMFAATLVYN
                     HRRTGEFIFDGRRFLLRRVYFPEQPTPEWYIIDLLQHHDMAGVSSAELEDRLVATLKH
                     QRWNQKRLRAMAEEYGTKTTIELIGRCLQKADEP"
     gene            complement(2184..2717)
                     /locus_tag="PKH54_11575"
     CDS             complement(2184..2717)
                     /locus_tag="PKH54_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00578.1"
                     /translation="MNTTSKSATPVTADSESARIPGICFVCEYNEGRSVHLELAVRDR
                     LRRRGLQSDLCSAGFTQGQRIHDFRRGFLREHSIPEAEIVGHRPTIFGAAHAGADLIL
                     VAELPMVERLLRKHPELRGRVMTIRGFAHGRNPDNDLDAAQAHIEDAAGHGMEEKVHL
                     YNELEALADLIAERLAR"
BASE COUNT          506 a          837 c          908 g          605 t
ORIGIN      
        1 gggcggcacc gccactgcat cctgccaggt tggttcgccc ggccccgccg gccccgccgg
       61 ccccgcccgc cccgtctgcc ctgcctgccc tgccagctcc gccagccctg cagatcccgc
      121 cggatccccc atcccctgcc gccacatctt cgcggtgctc gtcggccgcg ccgaactggc
      181 gcaccgtctt ctggaatttc ttctgtgacg gcgggtgtcg gtgtgtccgg tgcggcggtg
      241 tcgcctgaac gaaatttcag gaactacgct tagatccgcg aactcccaag ttgaagtact
      301 ggatgaactc agaaccacag gaggaaaaaa tgagcatcca gaacgaaaca gtggccctga
      361 atgtgaaacc gtttgcgacc gatctcgagt acctgaccgc ggaattcgcg tggatacagg
      421 cccgcaccag gcggatcgag gcgagtcagt tggcgatgaa ccaggccccg gcgctggcga
      481 agatgcagtc ggcaggcatg tggcctgcta ttactggtga ttagggttca gctttccgag
      541 ccatgtgcgg atcgtgtcgc aggcctcgcc gagagggatt cgtggtcccc agaacatcgg
      601 tgcgatcctg gcatacgccc gggcgatggc ggtggccttg tccgagtcgg taagcacgag
      661 ggccggttca tcctggcggg gaaactccgc gatatccttt cgattaagca tgtccagcac
      721 cagcgccatc gtggtcattc ccatgtcagg caggctatct tcggcgcgga tgatctgcgc
      781 ggcgtcctcg tagtgtcgga cgaaggtgtc cggctccatg tcgtcgcggc tatatcgtcg
      841 cgccatggcg tcgagctttt caaagagggt gaccatggga tggacgcaac gcacgtccag
      901 cggtcgattg tcttcatatt cgtcgagcag gttcagttga acgagttggg cgtgcacgaa
      961 ggagtcgagc ggccttctga cgcagggcac aacgcgtgcg tggccgactt caagccgtac
     1021 gaagggactc atggtctggc tgagttcggc gaggagcgca ccggggtagc gagccagata
     1081 gtcggcgctg cgtgcctgcc tgtcgatcct ggtcgtgtcc cgttccactt tgacgtcggg
     1141 caccacgaga gtggcggcga gagcgtcgta gaaagcgcgg cgtttatcca ccggcccctt
     1201 gttggtgctg gtccagtttg tgatgtcggg cagggcgtcg accgttccgc gctcgaccat
     1261 caggtcgagg tcctcggaaa atcgctggat caggccgaat cccttggaga gggatgtgcc
     1321 acccttgaac cagatgtcga ggccggttcg gtggatcgcc cacagcgtgt gtgtgaccca
     1381 gtagtccttt tcaacgagcg ccgcggcgat cccggttgac cgcgccacga tctgaacgag
     1441 ttgtgggaat tccggatcct ggtgaatgaa attcatcacg gttcatccgc tttttgcaaa
     1501 catcgcccga tcagttcgat tgtggtcttg gtgccgtact cttcggccat cgcccgcaga
     1561 cgtttctgat tccatcgttg atgtttcaaa gtggcgacca gtctatcctc cagttcggcg
     1621 gatgaaacac cggccatgtc gtgatgctgc aaaaggtcga tgatgtacca ctcgggcgtc
     1681 ggttgttcgg gaaaatagac tctgcgcagg aggaaacggc gaccgtcgaa gatgaattcg
     1741 ccggtccgtc tgtggttgta aacaagtgtc gctgcgaaca tcgcagtggt gccgagtccc
     1801 agggcattcc attttggcgg tccagtgatc acgaaggtgt cactgccaag aaagccgcgc
     1861 aggagttcat catcggacgg tggggcctgc ccaaaacggc tgtccaatgg ggcgtaaaac
     1921 aatccatggg ctgcctgctg cagcttgcct tcgcgaacga ggcggcgcgc aagccgtgtc
     1981 gggttctcgc cgaagcagcg aagttcccgg gttcgatacg tttttccggg ttccagcttt
     2041 ggttgtgcgg ccaggctcac ggcatcatcc gtcagaaatc taccgtaaaa aacatacagt
     2101 gttcgggttt gcgtgtcaag gccatgtgag tcattcagac tggttgcagc gggggctttc
     2161 ctgatggttg tcagcagccc gaactacctg gcaagccgtt ccgcgatcag gtcggccagg
     2221 gcttcgagtt cgttgtaaag gtggactttt tcttccatgc catgaccggc ggcgtcttcg
     2281 atatgggcct gggctgcgtc caggtcgttg tcggggttgc gtccgtgggc gaacccgcgg
     2341 atggtcatca cccgcccccg cagttccgga tgtttccgca gcaggcgttc gaccatcggc
     2401 aattcggcga ccaggatcag atcggccccg gcgtgcgccg ccccgaaaat ggtcgggcgg
     2461 tggccgacga tttcggcttc gggaatgctg tgttcgcgca ggaagccgcg gcggaagtca
     2521 tgtatccgct ggccctgggt gaagccagcc gagcacaggt cggactgaag tccgcgtcgg
     2581 cgaagcctgt cgcgcacggc cagttccagg tggacgctgc ggccttcgtt gtattcacag
     2641 acgaaacaga tgccggggat ccgggcgctt tcgctgtccg cagtgactgg cgtggctgac
     2701 ttcgatgtcg tattcatgaa actccctcca gtgccttcaa ggccgcaatc atatcaggaa
     2761 ccgtcagact ggacgccggc ggctggcagg tccgcagatt tgggatgtcg ttgtcggccg
     2821 cgaccatccc gcgttccttt ctgcaccgtc ccacgt
//
LOCUS       DAOPPA010000428         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38088_length_2854_cov_3.141122, whole genome shotgun sequence.
ACCESSION   DAOPPA010000428 DAOPPA010000000
VERSION     DAOPPA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38088_length_2854_cov_3.141122"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1272)
                     /locus_tag="PKH54_11580"
     CDS             complement(130..1272)
                     /locus_tag="PKH54_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD00579.1"
                     /translation="MRILQSIVLPAILILSLPALAQIPPVSGGMTRGAFSSARIEGRW
                     GVKVWSNRLLDISPREFGRCSAHPKHPVVVCGSADGYFRALHKDNGELIWTFKTSAAL
                     RAAPAFHGDQVILAAADGCLHTLDVRDGSAIWKKPYCVDNPIWGDPVVTPDIIYFSVL
                     GNQVYAIGREDGRLRFKVQRDRPQAMSSEGISEPVIDGDVLYSAFSDGVLVASNRFNG
                     RELWHQDLTDDSNGPTDADSTPVVKDGVVFSGAFSAGPVALKASDGTILWKGRRFGVD
                     QPVIFGRLLIVADAEGFVTAFNRADGTESWTTKLDTPAAWNPILVEDMLVVGGDRGLW
                     LLRPSDGKVLSRTALPFGVTTEPGVDRLDLYFPAPGGTVNKAALVR"
     gene            complement(1328..2101)
                     /locus_tag="PKH54_11585"
     CDS             complement(1328..2101)
                     /locus_tag="PKH54_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00580.1"
                     /translation="MSNKLSIKSEQPARDNLKGPDEFQQYVAKVADFFRVYGTRIAIA
                     AGAVVVVLIAVVLIGKVMKSSSLKTASEFSENFTKVSAQILPNGSMEGLPVANRQEVD
                     SARKALAEFAGRNEGKEIFGLARIGEGIAALQAGDAAGAADILKAVLDGGKLDPALVA
                     IANEAFAVAADQAGRREDAMKAYELIAAGNGRMSKVYALLHLGDMFNPAMKVSDDQPI
                     DAAKATDYYNRANEAAASISSFEAMMMQGTVKSRIALMQ"
     gene            complement(2153..2223)
                     /locus_tag="PKH54_11590"
     tRNA            complement(2153..2223)
                     /locus_tag="PKH54_11590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2189..2191),aa:Gln,seq:ttg)
     gene            complement(2335..>2854)
                     /locus_tag="PKH54_11595"
     CDS             complement(2335..>2854)
                     /locus_tag="PKH54_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00581.1"
                     /translation="VSGEVAGSRVMGAPQIYGGVTYFTSFHPSPGNLCIPGTGRLYGV
                     DYMRNTGSCDSPYPKLSLASDPFNFQTYIDIGDEIRGALPYGVVVVDRPSCFGSSSIY
                     DAGAGGASGNPLGTSGSGQPQLVVQTGVYTGEIGEQPAQGSTDRSINQIMRTIGQTMQ
                     SIVVGTWGAVFD"
BASE COUNT          603 a          884 c          805 g          562 t
ORIGIN      
        1 aatacaaaaa cgcacccgcc gaattttaca cactgcccgg tttcaaggcc agtaggggcg
       61 aatgaaaatt cgcccgtctg gaatgaaaat tcgccgccct ttgatgaaaa tcgaattgaa
      121 cctggggctt cagcgcacaa gggcagcctt gttaacggtt cccccgggcg ccgggaagta
      181 aagatccagt cggtcaaccc cgggttcggt tgtcactccg aacggaagtg ccgttcgcga
      241 aagaaccttt ccatcggacg gcctgaggag ccacagtccg cgatctccgc ccaccaccag
      301 catgtcctcg acgagaatcg ggttccatgc cgccggggtg tcgagtttcg tcgtccagct
      361 ctcggtgccg tcggcgcggt taaaagcggt gacgaatcct tccgcatccg cgacgataag
      421 cagacggccg aaaatcaccg gttgatcgac cccgaaacgc cgccccttcc agaggatcgt
      481 tccgtcggat gccttcagcg ccaccgggcc tgccgaaaac gcgcccgaaa aaacgacgcc
      541 gtccttcacc accggggttg aatccgcgtc ggtcggtccg ttcgagtcat cggtcagatc
      601 ctggtgccaa agttcgcgac cgttgaaacg gttgcttgcc accagcacgc catcggaaaa
      661 cgccgaatag agcacatccc catcgatgac cggctcgctg atgccttcgc tgctcatcgc
      721 ctgtggcctg tcgcgctgaa ccttgaagcg caggcggcca tcttctcgcc cgattgcata
      781 tacctggttc cccagcaccg aaaaatagat gatgtccggc gtcaccaccg gatcacccca
      841 gatgggattg tcaacgcagt aaggcttctt ccagatcgcc gagccatccc tgacatccag
      901 agtgtgcagg cagccgtcgg ccgccgcgag tatcacctgg tcaccatgga aggccggcgc
      961 ggcacgaagg gcagcggatg tcttgaatgt ccagatgagt tcgccgttgt ccttgtgcag
     1021 cgccctgaaa tacccgtcgg cggatccgca cacgacgacc ggatgcttcg ggtgggcgga
     1081 acaccggccg aattcccgcg gactgatatc cagcagcctg ttcgaccaga cctttacgcc
     1141 ccagcgccct tcaatcctcg cgctgctgaa ggcacccctt gtcattccac ccgagacggg
     1201 cggtatctgg gccagcgccg gaagcgacag gatcaggatt gccggcagga cgattgactg
     1261 aaggattcgc atcacaatga ttcctgtcag gcaggtgccg cacgcccggg tctccggacg
     1321 ataccgttca ctgcataagg gcgatgcggg acttaaccgt gccctgcatc atcatggctt
     1381 caaacgagct gatggaagcc gcggcttcgt tggcacggtt ataataatcc gtggccttgg
     1441 cggcatcgat cggctggtcg tcagagactt tcattgccgg gttgaacatg tcgcccaggt
     1501 gaagaagggc ataaaccttc gacattctgc cgttgccggc cgcgatcagt tcgtacgcct
     1561 tcatcgcgtc ctcgcgacgg ccagcctggt cggcggctac ggcgaaggcc tcgttggcaa
     1621 tcgcgacaag cgccggatca agttttccgc cgtcaagaac ggccttcagg atgtcagcgg
     1681 cgccggcggc gtccccggcc tgaagtgccg cgataccctc gccgatgcgt gcaagcccga
     1741 aaatttcctt gccttcgttc ctgcctgcga actcggcaag cgccttcctg gccgaatcaa
     1801 cttcctggcg gttggcaacg ggaagtcctt ccatcgatcc gttgggcagg atctgggccg
     1861 aaaccttcgt gaagttttcg gaaaactccg aggccgtctt caggctggaa gacttcatca
     1921 ccttgccaat cagaacgacg gcaatcagaa cgacgacaac agcgccggcg gcgatggcaa
     1981 tcctggttcc atagacgcgg aaaaagtccg cgacctttgc cacgtactgc tgaaactcat
     2041 ccggtccctt caggttgtcc ctggccggct gctcactctt gatcgaaagt ttgttcgaca
     2101 tgctttgcta ccttgaaagt gtcagttaat cctgtcaaaa atggggttcc agctctccgg
     2161 cagggattcg aaccccgata tcaggaacca aaatcctgag tcctgccatt ggacgaccgg
     2221 agaatcacaa atttccgctt ccggtcaccg cctgggcaac cgcaggatac gggacggtga
     2281 tttttagcac caaaaccgaa tgatggcaac tgttgaaggg tggcccatgc cggatcaatc
     2341 gaaaacggca ccccaggttc cgaccacaat ggactgcatg gtctgtccga tggttcgcat
     2401 aatctggttg attgatctgt cggtgcttcc ctgtgcgggc tgctcgccga tttcgccggt
     2461 atatacgccg gtctgaacca caagctgggg ctggcccgaa cctgacgtgc caagcggatt
     2521 tcccgaagcg ccgccggcac cggcatcgta aatgctgctg gaaccgaagc acgatgggcg
     2581 atccacgacc accacgccat agggcagggc gccccggatt tcatccccga tatcgatgta
     2641 ggtctggaag ttgaacggat ccgacgcaag cgacagcttc gggtatggcg agtcgcagct
     2701 tccggtattg cgcatgtaat cgaccccgta aagccgaccg gtaccgggaa tgcacaagtt
     2761 tccggggctg ggatggaaag atgtgaagta tgtgacgccc ccgtaaattt gcggggcacc
     2821 cataaccctt gacccggcaa cttcgcccga cacc
//
LOCUS       DAOPPA010000429         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38124_length_2852_cov_3.900965, whole genome shotgun sequence.
ACCESSION   DAOPPA010000429 DAOPPA010000000
VERSION     DAOPPA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38124_length_2852_cov_3.900965"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1812
                     /locus_tag="PKH54_11600"
     CDS             154..1812
                     /locus_tag="PKH54_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOD00582.1"
                     /translation="MHETIRKTLLRDLDAAADRVAVDLPRPELIRMAVERKDCVVSAC
                     GALATWTPELSTGRRPKDTVMVRRPCSEARIDWDSPNNLPIDETTFDMIFEDARKFLA
                     DKEQIFVTNRVVGASSEWAMPVMTISDSGLAAVFTDNMFRPVPEDIGTSVFAELPFTV
                     IALPYDQLDSERYLGRLRIDPDTKKTANVVIAIDYDRRMGIVIGSAYLGSIKKMVFTV
                     MNYYLPDYGILPLHCSANEGADGSSALMLGLSGTGKTTLSADPLRALLGDDEHGWGKN
                     GVANMENGCYAKLIDLNPAKEPEIFSAVFHEDDYQNHGSIIENAMMYPCGEFDLTDRR
                     LTENSRASYPLTYLSNIKPSSCADHPKTILFLTADANGVLPAIARLNRNQAMLWFLMG
                     YTSKLAGTETGVVSPSSTFSRFFGAPFMPRTPSTYADMLGKMMQEHDTDVFLINTGWI
                     GGRYGTVKRVDIDVTRAMVRDAVEGRLRDVNYRTDPLFKFQVPLACPSTSATDVLNPE
                     NMWADKAEFRAAAEKLAAEFKINFGKAFGNKGIAPEIVAECPGM"
     gene            1923..>2852
                     /locus_tag="PKH54_11605"
     CDS             1923..>2852
                     /locus_tag="PKH54_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00583.1"
                     /translation="MSYKHVLATSLTLALFSCGNPNLPPWLLDSGGGEDTGHDVVHPG
                     DSGGDSASDVIRDVPADEWEDAFDILAADDGIENDPSPETAAETWPDSEWDIESAVDT
                     EDDDGAIQEDSRDTTTTDEIEDIPEDIQPEVQHECERDLDCTIPVNLGVCERMKCIDF
                     TCVRQNHEDLSDCNDGNLCTTADKCLGGICTGATITCDDGNVCTNDYCVPSTGCATSA
                     RNNVPCDDGDLCTDNDRCSAKVCQGTLKSCTTPPAKACNTENTAVVTSSLPGQCVSGS
                     CQYAVTTTPCPGGCLNGSCVNTDPCAGKVCDTPP"
BASE COUNT          641 a          854 c          840 g          517 t
ORIGIN      
        1 gtcgatggga cgtatatcgg atttgtcgaa agcaattctc agcaggccag cgtgaccggg
       61 gactggtcgg cggtcaggat tgtcgaggac gaataatcat caatctgcga agtcctggaa
      121 cggttagtcc cgggatctga tggagaaagc aaaatgcacg aaactattcg aaagactctt
      181 cttcgggatc tggacgcggc ggcggatcgc gtggcggtcg atcttccacg cccggagttg
      241 atccggatgg cggtcgaaag aaaggactgc gtcgtttcgg cctgcggggc ccttgccacg
      301 tggacaccgg aactttcgac cggaaggcgg ccgaaggaca ctgtcatggt tcgtcgcccg
      361 tgcagcgagg cgagaattga ctgggactcg ccgaacaacc tgcccatcga cgaaaccacg
      421 tttgacatga tcttcgagga cgcccggaaa ttcctggccg ataaggaaca gatcttcgtg
      481 accaacaggg tggtcggggc ttccagcgaa tgggcgatgc ccgtgatgac catttccgac
      541 agcgggcttg ccgcggtatt caccgacaac atgttccgcc cggttccaga ggacattgga
      601 acaagcgtgt tcgcggaact tcctttcacg gtcattgccc tgccgtacga ccagcttgat
      661 tcggaaagat acctgggacg gctgaggatc gatcccgaca cgaagaagac cgccaacgtc
      721 gtaatcgcca tcgattacga caggcgcatg ggaatcgtga ttggttccgc gtacctgggt
      781 tccatcaaga agatggtttt cacggtcatg aattactacc tgccagacta cggcatcctt
      841 ccgcttcact gcagcgccaa cgagggggcc gacggcagca gcgccctgat gctgggactt
      901 tccggaaccg gcaagaccac gctttcggcc gatccgctgc gcgccctgct tggagacgat
      961 gagcacggat ggggaaagaa cggcgtggcc aacatggaaa acggatgcta cgccaagctg
     1021 attgacctga atccggccaa ggaacccgaa atcttttccg cggtcttcca cgaggacgat
     1081 taccagaatc acggttccat catcgaaaac gcgatgatgt acccgtgcgg ggagttcgac
     1141 ctgaccgaca gacgccttac cgagaactcc agggccagct atccgctgac ctacctttcg
     1201 aacatcaagc cgtcgtcctg cgccgaccat ccgaagacca tcctgttcct gacggccgac
     1261 gccaacggcg tgcttccggc aatcgcaaga ctgaaccgga accaggcgat gctgtggttc
     1321 ctgatgggct acaccagcaa actggccggg accgaaaccg gcgtcgtcag cccgagttcc
     1381 acgttctcca ggttcttcgg ggcacccttc atgccgcgga ctccttcgac gtatgcagac
     1441 atgctcggaa agatgatgca ggagcacgac accgacgtct tcctgatcaa taccggctgg
     1501 attggcggca gatacgggac ggtcaagcgt gtcgatatcg acgtgacgcg tgccatggtt
     1561 cgcgacgccg tcgagggaag gctgcgcgac gtgaattacc ggaccgatcc gctgttcaaa
     1621 ttccaggtgc ccctggcttg cccatcgacc agcgcaaccg acgttctcaa cccggaaaac
     1681 atgtgggccg acaaggcgga attcagggcg gcggcagaaa agctcgcggc cgaattcaag
     1741 atcaattttg gcaaggcctt cggcaacaag ggcatagcac ccgaaatcgt tgcagaatgc
     1801 cccggcatgt agtgggcggc tccctgcggg ccccgcatgc gcgcgcgttt cactgaaagc
     1861 accaaatcac tgtataataa cgtccagcgc cgattccagg actttccact ccgggggctc
     1921 ccatgtctta caaacacgta ctggccactt cgcttacgct ggcactcttt tcgtgcggca
     1981 acccgaacct tccaccgtgg ttgcttgatt ccggcggggg cgaggacacg ggacacgatg
     2041 tcgttcaccc tggcgactcc ggcggcgatt cggcctccga cgtgattcgg gatgtcccgg
     2101 ccgacgagtg ggaggatgca ttcgacatcc tggccgcgga cgacggcatc gagaacgacc
     2161 cttccccgga aaccgcggcc gagacgtggc cggactccga atgggacatc gagtcggccg
     2221 tcgatacgga agatgacgac ggcgcaatcc aggaggattc aagggacacg acaaccaccg
     2281 acgaaatcga agacattccc gaggatatcc agcctgaagt ccagcacgaa tgtgaacgcg
     2341 atttggactg cacgatcccg gtcaacctgg gcgtctgcga acggatgaaa tgcatcgatt
     2401 tcacgtgcgt ccgtcagaat cacgaagacc tgagcgactg taacgacggc aacctgtgca
     2461 cgaccgcgga caagtgcctc gggggcatct gcactggcgc cacgatcacc tgcgacgacg
     2521 gaaacgtctg cacgaacgat tactgcgtgc cttcaaccgg atgcgcgact tccgcaagaa
     2581 acaacgtccc gtgcgacgac ggcgacctct gcaccgataa cgacaggtgt tcggcaaagg
     2641 tctgccaggg aactttgaag tcctgcacta cgcccccggc aaaggcttgc aacaccgaaa
     2701 atacggcggt ggtcacatca tcgcttcccg gtcagtgcgt gtcgggctcg tgccagtacg
     2761 ccgttaccac aacaccatgt ccaggtggct gcctgaacgg aagctgcgtg aacacggatc
     2821 cgtgtgccgg gaaggtctgc gacacaccgc cc
//
LOCUS       DAOPPA010000430         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38547_length_2830_cov_4.699459, whole genome shotgun sequence.
ACCESSION   DAOPPA010000430 DAOPPA010000000
VERSION     DAOPPA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38547_length_2830_cov_4.699459"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /gene="purL"
                     /locus_tag="PKH54_11610"
     CDS             complement(<1..1704)
                     /gene="purL"
                     /locus_tag="PKH54_11610"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOD00584.1"
                     /translation="MNEQLKAFAVAGGLNEEEIAKILGFIGRDPTIAEIAAFSAMWNE
                     HVSYKSSRKYLKTFPTRGPRVLVGPGENAGVVDVGDGWAISFKMESHNHPSYIEPFQG
                     AATGVGGILRDIFTMGARPIANMDSLRFGSLSHPRTRYLVNGVVAGISYYGNCMGVPT
                     IGGELVFDPTYDGNCLINVFTLGVVKADGVFYGKAAGVGNPVIYMGPATGRDGMGGAS
                     MASAAFDEKAAEKRPTVQVGDAFCEKVVLECTLELMKSGLVLGIQDMGAAGLTCSTFE
                     MADRAKMGMKVDLSKVPQRAANMTPQEILLSESQERMLLVCERGNEEGVLEICRKWDI
                     PGAVIGEVTDTRQMEMWFNGEKVVDLPINLLTDEAPLYDRPWAEPAGLAERWKVPAFT
                     VGDLKDEFLGLIDDPSFRSKAPVFQKYDHMVQLRTVVRPGDGDAAVMRLLETGTNRGV
                     AMTSDGNSPACWLHPYNGAFNIIAEACLNLACVGARGIALTDCLNFGNPERPDVMWEF
                     KEVVAGLSEACIAFDTPVTGGNVSFYNETAGTSIQPTPVVGVVGLVEDCDRVAGTFFT
                     RE"
     gene            complement(1709..2428)
                     /gene="purQ"
                     /locus_tag="PKH54_11615"
     CDS             complement(1709..2428)
                     /gene="purQ"
                     /locus_tag="PKH54_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOD00585.1"
                     /translation="MRFAIPVFPGSNCDKDCLHVLGNVLGQDAFFVFHKEKTLPATDV
                     VVLPGGFSYGDYLRSGAIARFSPIMDDVIRFANAGGIVVGICNGFQIMLEAGLLPGGM
                     RLNTCGHFVCKDVWLRVENGETIFTRKAGKGAILGMPIAHAEGNYYAPPDLLKEIEDN
                     GQVLFRYCDQDGNVTPEMNPNGAVNNIAGIMNREGNCFGMMPHPERASECILGGDQGR
                     ILFESLIAAGGGSVCKCGCRG"
     gene            complement(2476..>2830)
                     /gene="purS"
                     /locus_tag="PKH54_11620"
     CDS             complement(2476..>2830)
                     /gene="purS"
                     /locus_tag="PKH54_11620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOD00586.1"
                     /translation="KIRRSTDGIGRCPALDRRVMAAFRKNVQQQGTAMAENVFNVSVV
                     VMPKAEILDPQGKAIMGGLKTMDFAEIQDVRIGKMVHLKLSGDSRETVMARATQMAKA
                     LLSNPVTEDFKVIVE"
BASE COUNT          489 a          910 c          867 g          564 t
ORIGIN      
        1 ctcgcgggtg aagaacgtcc ccgccacgcg gtcgcagtct tcgaccaggc cgacgacgcc
       61 gaccaccggg gtcggctgga tcgaagtgcc cgcggtttca ttgtaaaagc tgacgttgcc
      121 gccggtgacc ggggtgtcga atgcgatgca cgcttcggac aggccagcca cgacttcctt
      181 gaattcccac atgacgtccg ggcgttccgg gttgccgaag ttcaggcagt cggtcagcgc
      241 gatgccgcgc gcgccgacgc acgccaggtt caggcatgcc tcggcgatga tgttgaacgc
      301 gccgttgtac ggatgcagcc agcaggccgg gctgttgccg tcggacgtca tcgccacgcc
      361 gcgattggtt ccggtttcca gcaaacgcat gaccgccgcg tcgccgtcgc cggggcggac
      421 gaccgtgcgc aactggacca tgtggtcgta cttctggaac acgggggcct tggacctgaa
      481 cgacgggtcg tcgatgagac ccaggaattc gtccttcagg tcgccgaccg taaacgccgg
      541 aaccttccac ctttcggcca gtccggccgg ttcggcccac ggacggtcgt acagcggggc
      601 ttcgtcggtc agaaggttaa tcggcagatc gacgaccttt tcgccgttaa accacatttc
      661 catctggcgg gtgtcggtga cttcaccgat gaccgcgccg gggatgtccc acttgcggca
      721 gatttcaagg acgccttcct cgttgccgcg ttcgcagacc agaagcatgc gctcctgcga
      781 ttcggacagc aggatttcct gcggcgtcat gttcgcggcg cgctgcggta ccttcgacag
      841 atcgaccttc atgcccatct tggcgcggtc ggccatctcg aacgtcgagc atgtcaggcc
      901 tgcggcaccc atgtcctgga tgcccaggac cagcccggac ttcatcagtt caagcgtgca
      961 ttcaagcacg accttttcgc agaaagcgtc gccgacctgg acggtcgggc gcttctcggc
     1021 ggccttttcg tcgaacgccg cagatgccat cgaagccccg cccatgccgt cgcggccggt
     1081 cgccggaccc atatagatga ccgggttccc gacgcccgcg gccttgccgt agaagacgcc
     1141 gtcggccttc acaacgccca gcgtgaacac gttgatcagg cagttgccgt cgtaggtggg
     1201 gtcgaacacc agttcgccgc cgatggtcgg cacgcccatg cagttgccgt aatagctgat
     1261 gcctgcgacg acgccgttga ccaggtatcg cgtcctgggg tgcgacagcg aaccgaacct
     1321 cagcgagtcc atgttggcga tcggccgcgc gcccatcgtg aagatgtcgc gcaggatgcc
     1381 gccgacgccg gtcgcggcgc cctggaacgg ctcgatgtac gacgggtggt tgtgcgactc
     1441 catcttgaag gaaatcgccc agccgtcacc gacatcgacg acgccggcgt tctcgccggg
     1501 gccgaccagc acgcgcggtc cgcgggtcgg gaaagttttc aagtatttgc gtgacgattt
     1561 ataggaaacg tgttcgttcc acatcgccga aaacgccgcg atttcggcaa tcgtcgggtc
     1621 gcgcccgatg aagcccagga tcttggcaat ttcttcttcg ttcaggccgc ccgcaaccgc
     1681 aaaagcctta agctgttcgt tcatctggtc accccctgca tccgcacttg cacacggaac
     1741 caccgccggc cgcaatcagc gactcgaaca ggatcctgcc ctggtcgccg cccaggatgc
     1801 actcgctggc gcgttccggg tgcggcatca tgccgaagca gttgccttcg cggttcataa
     1861 tgcccgcgat gttgttgacc gccccgttcg ggttcatttc cggggtcacg tttccgtcct
     1921 ggtcgcagta acggaacagc acctggccgt tgtcctcgat ttccttcagg agatcgggcg
     1981 gggcgtaata gtttccttcc gcgtgggcaa tcggcatgcc caggatggcg cccttgccgg
     2041 ccttgcgggt aaatatcgtt tcgccgttct cgacgcgcag ccagacgtct ttgcagacga
     2101 agtggccgca agtgttcagg cgcatgccgc cgggcagcag gccggcctcg agcatgatct
     2161 ggaagccgtt gcagatgccg acgacgatgc cgcccgcgtt ggcgaagcgg atgacgtcat
     2221 ccatgatggg cgaaaagcgc gcgatggcac ccgagcgcag gtagtcgccg tacgaaaacc
     2281 cgcctggcag cacgaccacg tcggtcgcgg gcagcgtctt ttccttgtgg aacacgaaga
     2341 aggcgtcctg cccaaggacg ttgccgagca cgtgcaggca gtccttgtcg cagttggaac
     2401 ctgggaatac cgggattgcg aatctcatct ggtccgtctc ctgccagtga atgtcgtggc
     2461 aaaccgccgc cggttctact cgacgatgac cttgaagtcc tcggtgaccg ggttcgacag
     2521 cagggccttc gccatctggg tcgcccgtgc catcacggtc tcgcggctgt cgccggacag
     2581 tttcaggtga accatcttgc cgattctgac atcctggatt tcggcgaagt ccatggtctt
     2641 cagaccgccc atgatggcct tgccctgcgg gtcaaggatt tcagccttcg gcatcacaac
     2701 caccgatacg ttaaagacgt tttctgccat tgcggtcccc tgttgttgaa cgtttttccg
     2761 gaatgctgcc attacgcgcc gatccagggc cggacatcgt ccgatcccgt ccgtgctgcg
     2821 gcggatttta
//
LOCUS       DAOPPA010000431         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38590_length_2827_cov_4.577201, whole genome shotgun sequence.
ACCESSION   DAOPPA010000431 DAOPPA010000000
VERSION     DAOPPA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38590_length_2827_cov_4.577201"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2827)
                     /locus_tag="PKH54_11625"
     CDS             complement(<1..>2827)
                     /locus_tag="PKH54_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00587.1"
                     /translation="FSHDLFQAALSIASIMQERGISDLEQLGIVLQACAKSGAPDRAC
                     SAAESCLKRCVGADVETISGAMSRNGLKRQAAEFARGNLASMKDRGRAFLVLSKLWMA
                     AGEKDAAVDSMAAWVRGDPIDGPRGGVAGVAKPDVERLVEGASIFLAERKFSLCLKLL
                     DTFNGLAPAGSSNADEIAFLRVSSLVGRKDWDGAIRAANESVNRAGRRESLAVRLSQV
                     FFDARRLRESADILAAGRPNGIAADEQARSRNHLMAARLIKAGVMKGDIPGEIRAALE
                     SAGDDHDVVAAIARFVKDLGLPPEIEILAYRKLAAREPGNPAPLARLVDLYLDNGQED
                     DAVDALGTMFLVGGKSSAAVVQAIDRFLAARFFGGAFLVAAQAGVADLPPGVARRLSD
                     ACLRIGERECVRKYLPVFLAGPVVDDYNYLELSEGLISGGFLDLAADALDVAAKAVGA
                     DALWRVSVLKGRIALVSRKLPDAARFFEEAVRDQSDNGSIAVAVSSEYSKAGRIADAA
                     AWLQRSFTETSGFMRPQAYPILVNTLRRLGLSSEINLEPLFKFAAVGSSPESLVYSDD
                     DGRAHRLWDGRRVFEVVDALVGAGRLDLALQFAVRMLGSRPEGALKLIELAGLAGDTK
                     GALDTARKICRRNPSDKAPSCGDACMALADIGLHNQGWDLFLEALKKHPERLSGRDAA
                     ALVSMAVRKGDGDVALQAARLLSPEIYKNAARIEDVWPVLPELLGRDRWTSLLADLAA
                     REDMAIDGRILVEAGINLIIAGRREDGLALVQKYSAEGPRMLGVAFMHLVTAGLFRDA
                     VGLLEGARDELVAGMTQQDLFVILSSFASRGDLAGAGAFASRYIRASGGSLDAGRAAA
                     FASLAYTDATSVIADIEKVGIDGLALRERIQYAAMLWAIGERDRAVGEFRKVLESDGE
                     PEEISAYRRFVFGFLESEAAVDVISGLF"
BASE COUNT          578 a          971 c          777 g          501 t
ORIGIN      
        1 ggaaaagccc ggaaatcaca tcgaccgcgg cttccgactc aagaaacccg aaaacgaatc
       61 tgcgatatgc cgaaatctcc tcgggctcgc catccgattc cagaaccttc ctgaattcgc
      121 cgacggcccg atccctttcc ccgattgccc agagcatcgc cgcgtactgt atgcgttcac
      181 gcagcgccag cccgtcgatg ccgacctttt cgatgtcggc gattacggaa gttgcgtcgg
      241 tgtaagccag ggaagcgaag gcggcggccc tgccggcatc gagtgatccg cccgaggcgc
      301 ggatatagcg cgaggcaaag gcaccggccc cggccagatc gccccgggat gcaaatgaac
      361 tcagaataac gaacaaatcc tgctgggtca tgccggcgac aagttcgtcc ctggcaccct
      421 cgagcaggcc caccgcatcc ctgaaaaggc cggctgtcac aagatgcatg aaggcgacgc
      481 caagcatgcg cggcccttcg gccgaatact tttgaaccag ggccaggcca tcttcgcgcc
      541 ggccggcaat tatcaagttg attcctgcct cgaccaggat tcttccgtcg atggccatat
      601 cttcgcgggc tgccaggtcg gccagcagcg acgtccacct gtcgcggccg agcaattcgg
      661 gaagaaccgg ccacacgtcc tcgatgcggg ccgcattctt gtatatctcg ggtgaaagaa
      721 gccgcgcggc ctgcaaggcc acatctccat cccccttcct taccgccatg cttacaagag
      781 ccgcggcgtc acggcccgaa agtcgctcgg gatgcttttt caaagcctca agaaacagat
      841 cccatccctg gttgtgcaga ccgatatccg caagcgccat gcacgcgtcg ccacacgacg
      901 gggccttgtc cgaagggttc cttctgcaga tcttgcgtgc cgtgtccagg gcgcccttcg
      961 tgtcgccggc aagcccggca agctcgatca gcttcagcgc gccctccggc ctgctgccga
     1021 gcatcctgac ggcaaattga agcgcaaggt ccaggcgacc ggcgccgacc agcgcatcaa
     1081 cgacttcgaa gacacgcctg ccgtcccaca agcgatgcgc ccgaccgtcg tcgtccgagt
     1141 acaccaggct ctccgggctg cttccgaccg ccgcgaactt gaaaagcggt tccaggttta
     1201 tctccgacga aagcccgagc ctccgcagcg tgttaaccag aatcggatag gcctgtggcc
     1261 tcatgaaacc ggatgtttcc gtgaacgatc tctggagcca tgccgcggca tcggcgattc
     1321 gaccggcctt tgagtactcc gatgaaacgg cgaccgcgat ggaaccgttg tcggattgat
     1381 ccctgacggc ctcctcaaag aatctggcgg catccggcaa ttttctcgaa accagcgcga
     1441 tccgcccctt cagcacgctt acgcgccaaa gcgcatccgc accgacggcc ttcgccgcaa
     1501 cgtccagggc atctgcggca aggtcaagga atcccccgga aataagtccc tcggacaact
     1561 ccaggtagtt gtagtcatcg acgacaggcc ccgccagaaa tacgggcagg tacttcctta
     1621 cgcattctcg ctcgccgatt cgcaggcatg cgtcggagag ccgcctggca acgccaggtg
     1681 gaaggtcggc cacgccggcc tgcgcggcga ccaggaaggc accgccgaaa aaccttgccg
     1741 caaggaaccg gtcaattgcc tgcacaaccg cggcacttga ctttcccccg acaaggaaca
     1801 tcgttccaag cgcgtcaacg gcatcgtctt cctgcccgtt atccagataa agatccacca
     1861 gccgcgcaag tggcgccggg ttcccgggct cgcgcgccgc cagcttccgg taagccagga
     1921 tttctatctc gggcggcagt ccaagatcct tcacaaaccg ggcaatcgcc gccaccacgt
     1981 cgtgatcgtc ccccgctgac tcgagggccg cccgaatctc accggggata tcgcctttca
     2041 tgaccccggc ctttatcaac cttgccgcca tcagatggtt tctggaacgt gcctgctcgt
     2101 ccgcggcgat gccgttcggc cttcccgccg caagaatatc cgcggattcc ctgagtctgc
     2161 gggcatcgaa gaagacctgg gagagcctga cggcaaggga ttcccttcga ccggctcgat
     2221 tcacggactc gttcgcggct cgaatggccc catcccaatc cttcctgccg acaagggaag
     2281 agacccgcag aaacgcgatt tcatccgcat tggaggagcc ggcgggggcc aggccgttga
     2341 atgtgtcaag cagcttcaga cagagactga atttcctttc agccaggaag atcgaagcac
     2401 cctcgaccag tctctccaca tccggcttgg ctacgcccgc cacgccgccc ctgggaccgt
     2461 cgattggatc gcccctgacc cacgccgcca tcgagtcaac ggcggcatct ttctccccgg
     2521 cggccatcca gagcttggaa agaaccagga acgcccgccc gcgatccttc atggaagcca
     2581 ggttgcccct tgcgaactcg gccgcctggc gcttcaggcc attacgagac atggcccccg
     2641 aaatcgtctc cacatcggct ccgacgcagc gcttaaggca actttcagcc gcgctgcaag
     2701 ccctgtcggg tgccccggac ttcgcgcacg cctggagcac gatgccgagt tgttccagat
     2761 cggaaattcc acgctcctgc attattgatg caatcgataa tgccgcctgg aacagatcat
     2821 ggctgaa
//
LOCUS       DAOPPA010000432         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38642_length_2824_cov_8.151679, whole genome shotgun sequence.
ACCESSION   DAOPPA010000432 DAOPPA010000000
VERSION     DAOPPA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38642_length_2824_cov_8.151679"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2377
                     /locus_tag="PKH54_11630"
                     /pseudo
     CDS             71..2377
                     /locus_tag="PKH54_11630"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
BASE COUNT          593 a          796 c          890 g          545 t
ORIGIN      
        1 gattctggta aggggcgggc aacagggtgg cgaggcggcg taaaaaggcc acgggaatca
       61 acccgttgcc cgcatggttg tcagtcagga aacttgcgca tgtggcgata ccgtctcgtt
      121 tgatggcagc acatcggaca attccgcccc gggccgcgac atcgtcgaat acgcgtggga
      181 tttcgatttc gatggaacaa gtttcgatgt cgatgcgaca ggggcaacca cgacacatgt
      241 attcggcagt ttcggccatt tcacggtcgc gcttcgggtt acggacaaca acgaccccgc
      301 catgaccgac atcacgacca ggttagtctc ggtgacgatt ggcaacaggc cgccaacggc
      361 agacgcgggt gggccgtatg tgatgatcgc cggcgatccc cttgaaatcg atgccggcgc
      421 ctcgttcgaa cccgatgggg cgtgcggcga tcgaatcgtt tcggtgctgt gggatctgaa
      481 cgatgacggt tcattcgacg acggatccga atccaccatg tcgctttcct gggccgacgt
      541 ggaagccttt ttgtgcgatg gaacatgccg gcttcccaac cgttacagaa tatcggttca
      601 ggtctttgac acgctcggcg aaacaagtgt cgctgaccag gtggtcagca tcattctgcc
      661 gttggcggac gaatgtggca acgggcgttg tgacgccttg gagaccgcga cagattgccc
      721 gattgactgt gaccggacaa aagttggagt gtccactggc cccggtgacc ttgccacgct
      781 gacatccgcg gccgaaagac tgggggcccg ctccttcagc gtggaactac attcctcaag
      841 catgatgctg gatgattcga acttggactt ccatgacctt acggacttcg tgcaaacaga
      901 caaagcggcc ggaagcttcc ttgtccgaac cagattcgag gcagacgaga tcgattttgc
      961 gacaatgaac ccggaaagcc ctttcgccag gtcaattgca gggctgtctg aagtcacctc
     1021 gggacacacc gcctatttct cgactccggt ttcatgggat ggcccgaccc acctgacggt
     1081 aacaccgacc agttacgaaa aactgataag gatttcagcc ggtgcaatac gttcgggcaa
     1141 cagatcggct gggctggtca ttggcgactt cgcgttcagt aacagccagg ccgagcaggc
     1201 atggtggaca gaattgctgg atctgatcga acagcgacag accgcgacca tgttcgacat
     1261 cctcggggtg cggttgttcg gcagtcccga tgaaatcgcc ggacagatcg ggtggctccg
     1321 gcaagccctg gatacgtggc caggcacggc gggaaaaccg atctggatta ccggcacggg
     1381 cgggccgacg ccttcccaga tggcctacac gtgccctgcc gaggcccaga acctgatcgc
     1441 cgccgccgcg attgagccgt gcgtcttcgc cggcgatctg accgattacc cggaagcaat
     1501 gcagttgttt tcattcgccc cgggaaggga ttcgacactg gacgaaaaac gcgaccgcat
     1561 gcaggcgcgc gagcaggttg aaagattcct gatcggcctg acttccggtg ccgatcgcgt
     1621 ctggtggcac gccatttcat caccgccggt tcatgaatgc gaaacaccga caggcgatat
     1681 cgaacttgga aagctgcgcc tggttgatgg tggattcgac ggcctgccga cattcgacct
     1741 gccgcctttc gggtacatgc gaaagactct tgaatggctg ggacagccga tggcagtcac
     1801 caggataaac atcggcggcg attcgaacct gatgttgttt gaagtgaccc ggcaggatga
     1861 cgaaaaggtc ctggtcgcgt ggcagaaacg ggacaggtac agtggcgagg accttccgtc
     1921 cgaagcgatc gaaatccctg tcgaatgggc cagggccagg gtcgaaagcc ttttcggtga
     1981 tgaagacctg attctgaccg ggcccggttc gatcacgctg aatttgaata acgatccggt
     2041 tcttgtttcc cgggcagagg acccgatccc ggacaccccg atcgttgaga cggtggaaga
     2101 atcgatggtc gaatcatccg aagattgctg cggggatgac tccaggttcg acgcatccct
     2161 ggattcaggc gcggattcaa tcaacaaatc cgacgtagtc gatgcgaccc aggacgactt
     2221 gaacgatcat ccggtcacac ccgatggcgg ttgcagtgcc gttcatgccg cgtcatcgca
     2281 ctggacggcg ctgccagcga ccttgttgct gctggtgctg cttctggcga ttgcggcgat
     2341 acgccgcttc ggacgtctcg gcaattcatt tcgttgacag gcaggaactg atgggcgctg
     2401 cgggccttgg ggcaacaggg acagggacag ggacagggac agggacaggg acagggacag
     2461 ggacagggac aggtgttggc catcacggct agatctggag ccaccgccgt gtccgtgccg
     2521 aacctgccca gcctgaattc agagcgtaaa cccacagcgg cgcagcgggt acccgtcacc
     2581 cggggaactc tcggaggcgc gcgacaccct gaggggcatt ggtgtcttcg tcgcgcgccg
     2641 agggcgtagc ggcgcggatg gggccaggcc ccgtccgcga ccgcgtgccc cggggatggg
     2701 taccccgagc cgcctgcgag cgaagcgagc tttactgcgt cagacacagg gcgggtgatc
     2761 ctctggtatt gcgtatagga actttcatcc acaaaacgcc tccatgggac gagtatggtc
     2821 gggg
//
LOCUS       DAOPPA010000433         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38742_length_2820_cov_3.734539, whole genome shotgun sequence.
ACCESSION   DAOPPA010000433 DAOPPA010000000
VERSION     DAOPPA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38742_length_2820_cov_3.734539"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..987
                     /gene="cysK"
                     /locus_tag="PKH54_11635"
     CDS             22..987
                     /gene="cysK"
                     /locus_tag="PKH54_11635"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOD00588.1"
                     /translation="MTAIFEDNSRSIGNTPLVRLNRITQGAAATVVAKIEGRNPGYSV
                     KCRVGTAMIDAAERDGILKRGDRSVTIVEPTSGNTGIALAFVSAARGYRLILTMPETM
                     SIERRKMLQAFGAELVLTEGAKGMKGAISKAEEIVASDPDSFFMPQQFRNPANPEIHF
                     NTTGPEIWQQTGGAIDIFVAGVGTGGTITGVSRYIKGVAGKKIASVAVEPAASPVLTA
                     LMAGQQPVPGPHKIQGIGAGFKPDILDLGIVDSVQAVTNEEAIEYGRRLHLEEGITAG
                     ISSGAAVAAAHRIAMDPANAGKLIVVILPDSGERYLSTALFDFGS"
     gene            1002..1445
                     /locus_tag="PKH54_11640"
     CDS             1002..1445
                     /locus_tag="PKH54_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD00589.1"
                     /translation="MEGLSTRGRYATRVMLYLVGRGRGDPVRISEISTAEAIPQNYLE
                     QLLVRLKVNGFVRSVRGARGGYVISCDPASVTVMDILEAVEGPIQLAPCIRETCTRGV
                     TCVTRGLWREASDAMNDVFRSYSLQSLADRVKEIETQSREPDYSI"
     gene            complement(1433..>2820)
                     /locus_tag="PKH54_11645"
     CDS             complement(1433..>2820)
                     /locus_tag="PKH54_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOD00590.1"
                     /translation="VDWTAYDEFMQPRIDGTYFDDSVGIARFDAGMFAPGHGTNGWTD
                     EQYKQAARAYVEHLDDKGWLDMMWTYSTDEPWLNGGEETWNRVIREAGLLHEASPLWD
                     GHVLVTGPMYPGAEEVVDIWCPVNPMYDKWFWFDMDYAGRGDYDEHIANNGELWFYNC
                     NGNFPPYPGYDIDTAIGYEPRILKWGSWYEKATGFLHWSLNIWYYVEPWIQWQSRDVF
                     GAMFARNGDGMLMYPGNDDGIHFDTEGNPLPTGIPAYLKIDGPVMSYRIKQIRDGLED
                     WELFIMAEKAGLGDWTRSTMRKVYRRFGDFFVEDCTKEYHYCPSKGEPWTLEYYKMQT
                     VRVLIIKKLLFTLYPDRYTDPEAPEIPDTVETPDTDDTGGPVPDAMIDDVIQPESMGD
                     AFADTLAPDAAVDSSPARDTPADTAAPGDTEHSSGGGCSQGQGSASPLALLLSIGLAC
                     LAAVRRRRHIE"
BASE COUNT          551 a          833 c          863 g          573 t
ORIGIN      
        1 aaattcaaaa cgaggtgtat cgtgacagca atcttcgaag acaattcaag gtcgattgga
       61 aatactccac ttgttcgcct gaaccggatt acacagggcg ccgccgccac tgtggttgcc
      121 aagatagagg ggcggaatcc aggatactcc gtcaaatgca gggtcggcac ggccatgatt
      181 gacgccgccg agcgtgacgg gatcctgaag cgtggcgatc gctccgtgac cattgtcgag
      241 ccgaccagcg gcaacaccgg gatcgccctt gcttttgtct cggcggcccg cggataccgc
      301 ctgatcctta ccatgccgga aacgatgtcg attgaacgac gcaagatgct gcaggccttc
      361 ggggccgaac ttgttctgac cgagggcgcg aagggcatga agggcgcgat atcgaaggcg
      421 gaagaaatcg tcgcttccga ccccgactca ttttttatgc cacagcagtt ccggaatccc
      481 gcgaaccccg agattcactt caacaccacc ggccccgaaa tctggcagca gaccggcggg
      541 gccatcgaca tattcgttgc cggggtcggc accgggggca ccattaccgg cgtaagccgt
      601 tatatcaagg gtgtcgcggg caagaagatt gcctcggtcg cggtcgaacc tgccgcgtcg
      661 ccggtactga ccgcccttat ggccggacag cagcctgttc cggggccgca taaaatccag
      721 ggaatcggtg cggggttcaa gccggacatc ctggatctgg gcattgtgga ttccgtccag
      781 gccgtgacga atgaagaggc catcgaatat ggccgacgcc ttcacctgga agaaggaatt
      841 acggccggga tttcaagcgg cgccgccgtc gccgccgcgc acaggatagc catggatccc
      901 gcaaatgcgg gaaagttgat cgtggtcatc ctgccggatt ccggcgagcg ttacctgagt
      961 acggcattgt ttgatttcgg ctcataatcg gggggttact gatggaaggc ctttcgacaa
     1021 gggggcgtta cgccacgcgg gtcatgcttt acctggtcgg tcgcggccgg ggtgacccgg
     1081 ttcggatatc cgaaatctcg acggccgagg cgattcccca gaactatctc gagcaacttc
     1141 ttgtccggct gaaggtcaac ggattcgttc gcagcgtgcg tggcgcccgt ggcggatatg
     1201 tcatttcgtg cgatccggcg tcggtgacgg tgatggacat cctggaagcc gtggaaggcc
     1261 cgatccagct tgcaccatgc atcagggaaa cctgcacgcg tggcgtcacc tgcgtgacca
     1321 ggggcctgtg gcgggaagca tcggacgcga tgaacgacgt gtttcgttca tattccttgc
     1381 agtcactggc cgatagagtg aaggaaatcg agacccagtc ccgggaaccg gactactcga
     1441 tatgacgccg gcgtcggacg gccgccaggc acgcaagtcc aatcgacaga agcagcgcca
     1501 ggggtgaagc ggaaccttga ccctggctgc aacctccacc ggacgaatgc tccgtgtcgc
     1561 cgggggcggc cgtgtcggcg ggcgtgtccc gggcggggct cgaatccacg gcggcatccg
     1621 gcgccagcgt gtccgcaaag gcgtcgccca tcgattccgg ctggattacg tcatcaatca
     1681 tcgcgtcggg aaccggcccg ccggtgtcgt cggtatcagg cgtttcgacc gtgtccggaa
     1741 tttccggggc ttcaggatcg gtgtaacgat cgggataaag cgtgaaaagc agcttcttga
     1801 tgatgagtac acgaactgtc tgcattttgt aatattcaag cgtccacggt tcgccctttg
     1861 acgggcagta atgatattcc ttggtgcagt cttccacgaa aaagtccccg aagcgcctgt
     1921 aaacctttct cattgtcgac cgcgtccagt cgcccagacc ggccttttcg gccatgatga
     1981 aaagttccca gtcctccagg ccgtcccgga tctgcttaat gcgatagctc atcaccggcc
     2041 cgtcgatctt aaggtaggcg gggattccgg tcggcagcgg gttgccttcc gtgtcgaagt
     2101 ggatcccgtc gtcgttgccg gggtacatca gcatgccgtc gccgttgcgc gcgaacatgg
     2161 cgccgaatac gtcgcgcgac tgccactgga tccaaggttc cacgtaatac cagatgttca
     2221 ggctccagtg caggaatccg gtggcctttt cgtaccagga tccccatttc aggattctgg
     2281 gttcgtaccc gattgccgtg tcgatgtcgt acccggggta aggcgggaag ttcccgttgc
     2341 agttgtaaaa ccacagttcg ccgttgttgg cgatgtgttc gtcgtagtcg ccgcggccgg
     2401 cgtagtccat gtcgaaccag aaccatttgt cgtacatcgg gttgaccgga caccagatat
     2461 cgacgacttc ctcggcgccc ggatacatcg gcccggtgac caggacatgg ccatcccaca
     2521 gcggggacgc ctcgtgcaga aggccggcct cgcgaataac gcggttccag gtttcctcgc
     2581 cgccgttcag ccacggctcg tcggtcgaat acgtccacat catgtccagc cagcccttgt
     2641 catcaaggtg ttcgacgtac gccctggccg cctgcttgta ctgttcgtcg gtccatccgt
     2701 tggtgccatg gccgggggcg aacattcccg cgtcgaacct ggcgatgccg accgagtcat
     2761 cgaaatatgt cccatcgatg cgtggctgca tgaactcgtc gtacgcagtc caatcgaccg
//
LOCUS       DAOPPA010000434         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38880_length_2812_cov_3.060210, whole genome shotgun sequence.
ACCESSION   DAOPPA010000434 DAOPPA010000000
VERSION     DAOPPA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38880_length_2812_cov_3.060210"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..908)
                     /locus_tag="PKH54_11650"
     CDS             complement(171..908)
                     /locus_tag="PKH54_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD00591.1"
                     /translation="MKNVLILGASSGIARQFERLVCADSRLVLAGNDPDVMAKIATDL
                     EVRSDGRRPSIFEFDSANIAGHAAFVQQVVAEVKHIDEVYLFFGVMFDEIECHRKPEL
                     AAKMGLVNYIGAMSILEHLANHMEANKAGLIVGVSSVAGDRGRASNYLYGSTKAGLSA
                     YLSGLRARLAAHGVHVCTIKPGFVDTPMTAHLKKGGLTSSAEKVARGVVQAARKRKNT
                     AYVPGFWWMVMMVIIHLPEFIFKKVKF"
     gene            complement(910..2289)
                     /locus_tag="PKH54_11655"
     CDS             complement(910..2289)
                     /locus_tag="PKH54_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD00592.1"
                     /translation="MDRIDLTRFPMTKISGFGRYPTSPARIMPVWEDFEITRALKARG
                     DFKFLARGTGLSYGDASLNRNNVCVSMRSMDRVLGFDHANGIIHVEAGVTLREILSFV
                     IPKGWFLPVTPGTAHPSIGGSVACDVHGKSKLPMHNYIRRLHMILADGSPIVCSPSEN
                     PDLFFATVGGMGLTGVITSVELELQSIKSSWVRYEGTRAANLDEIFSQFKDADDWPMT
                     VAWIDCVARGAKLGRSIMMKGRFAEPGDLKKGREPLHVPQKLKLAVPFDFPSFTLNKL
                     TVSAFNMLYNGKHPKRLETIMDYDSFFYPLDAIAHWNRIYGRPGLLQYQFLIPPEQGF
                     EGVKSVLEMIAATGKASFLAVLKKFGPMQNQGLLSFPTPGYFIALDFPYGNGSILEQM
                     ELWDKRVLEYGGRLYLTKDARMSRETFRAMYPRVDEFLSVKAKYDPDCVFRSDMAYRL
                     GLVQRERRV"
     gene            complement(2276..>2812)
                     /locus_tag="PKH54_11660"
     CDS             complement(2276..>2812)
                     /locus_tag="PKH54_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00593.1"
                     /translation="TITWTYVPGHSAADVLFRIEGLPLVITGDHVLPHVTTSPAVDVA
                     DGPEYAVSLQRYRDSLGYTSTLGNLIGCAGHGPVFSELGRRASALLATQDRRMERAAR
                     VIAKLGPCTALQVAGGLFGMEYRFEMLMFANECAGYARLLQQRGVCEIRREQGLPDTI
                     IPLQSPAEADKAGQDGQD"
BASE COUNT          574 a          864 c          805 g          569 t
ORIGIN      
        1 ctggccccgt gcgcgaccgc gattcccggg acgggtaacc ccgaaggacc cccaggtgca
       61 aggaacaatg ggcgtacggc agctcctcgt cccctgagcg aagcgaaggg ccccacgggc
      121 gaatcctgat ccgtacgggc gaattttcat tcgcccatac aagccgcaag tcagaacttg
      181 actttcttga agatgaattc gggcaggtgg atgatgacca tcatgaccat ccaccagaag
      241 ccggggacgt atgctgtgtt cttgcgcttg cgggcggcct ggacgacgcc gcgggccacc
      301 ttttcggcgc tggatgtaag cccgcctttc ttgaggtggg ccgtcatggg cgtatcgaca
      361 aatcccggct tgatggtgca gacgtgaacg ccgtgtgccg ccagcctggc gcgcagcccg
      421 gaaagatagg ccgaaagccc ggccttggtc gagccgtaca ggtaattgct ggcgcggccg
      481 cggtcgcccg cgacgcttga aacgccgacg atcaggccgg ccttgttggc ttccatgtga
      541 ttggccagat gttccaggat cgacatcgcg ccgatgtaat tgaccaaacc catcttggcg
      601 gcaagttccg gcttcctgtg gcattcgatc tcgtcgaaca tcacgccgaa gaacaggtac
      661 acctcgtcga tgtgcttcac ctcggcgacg acctgctgga cgaaggcggc gtggcccgcg
      721 atgttcgccg aatcgaactc gaaaatcgac ggacgccggc catcggacct gacttccaga
      781 tccgtcgcga tcttcgccat cacatcagga tcgttcccgg caagaaccag cctggaatcc
      841 gcgcagacaa gccgttcaaa ctgcctcgcg attccggacg acgccccaag tatcagcacg
      901 ttcttcatgt caaacccgcc tttcccgctg gaccaggccc agcctgtatg ccatgtccga
      961 ccggaacacg cagtccgggt cgtacttcgc cttcaccgac aggaattcgt caaccctggg
     1021 atacatcgcc ctgaaagtct cgcggctcat ccgggcgtcc ttggtcagat acagccgtcc
     1081 accatattca agaacccttt tgtcccagag ctccatctgt tcaagtatcg aaccgttgcc
     1141 gtacgggaag tccagtgcga tgaaatatcc cggcgtcgga aacgacagca gtccctggtt
     1201 ctgcatcggt ccgaactttt tcaggactgc caggaaggac gcctttcctg tcgccgcgat
     1261 catttccagg acggatttga ccccctcgaa tccctgttcc ggtgggatca ggaactggta
     1321 ctgcagcagt cccggcctgc cgtaaatccg gttccagtgt gcgattgcgt ccagcggata
     1381 aaagaacgaa tcgtagtcca tgatcgtttc aaggcgcttc gggtgtttgc cgttgtacag
     1441 catgttgaaa gcggaaacgg tcaatttgtt cagggtgaac gacgggaagt cgaacggcac
     1501 ggccagctta agcttctgtg gcacgtgaag aggctcgcgt cccttcttca ggtcgccggg
     1561 ctccgcgaaa cgtcccttca tcatgatgct gcgtccaagc ttcgcgcccc tggcgacgca
     1621 gtcgatccag gcaaccgtca tcggccagtc gtcggcgtcc ttgaactgtg aaaagatttc
     1681 gtccaggttc gcggccctgg tgccttcgta ccttacccag cttgacttga tcgactgaag
     1741 ttcaagttcg acgctggtga tgacgccggt aagtcccatc ccgccgacag tggcaaagaa
     1801 aaggtccggg ttttccgaag ggctgcatac tattggcgac ccatccgcca ggatcatgtg
     1861 aagccgcctg atgtaattgt gcatcggcag cttgcttttg ccgtgaacgt cgcatgcgac
     1921 cgacccgccg attgagggat gcgccgtgcc gggcgtcaca ggaagaaacc acccctttgg
     1981 aatgacgaag gaaaggattt cgcgcaatgt cacgccggct tccacgtgga tgatgccgtt
     2041 tgcgtggtca aatccaagga cccgatccat tgaacgcatc gacacgcaga cgttgttgcg
     2101 gttaagggac gcgtcgccgt atgaaagacc ggttccccgg gcaaggaact tgaagtcgcc
     2161 acgcgccttc agagccctgg tgatctcgaa gtcctcccag accggcatga tcctggccgg
     2221 ggaagtcggg tatcgcccga atccggaaat ttttgtcatc gggaatcgcg tcagatcaat
     2281 cctgtccatc ctgacctgcc ttgtcggctt ccgccggcga ttgtaacggg attatcgtgt
     2341 caggtaaccc ctgttcgcgt ctgatttcgc atacgccgcg ctgttgcagc agccgggcgt
     2401 aaccggcaca ttcgttcgcg aacataagca tttcgaacct gtattccatg ccgaacaggc
     2461 cgcccgcgac ctgcagcgcg gtgcacggac ccagtttcgc gatgaccctt gccgcccgtt
     2521 ccatcctgcg atcctgggtg gccagcaatg ccgacgcccg ccgtcccagt tccgaaaaga
     2581 ccggcccgtg accggcgcag ccaataagat tgccaagagt cgatgtgtaa ccaagcgaat
     2641 cgcggtaacg ctgcagagag acggcgtact cgggcccatc cgcgacatcg accgctggac
     2701 ttgtcgtcac atgcggcagc acatggtcac cggtgatgac caaaggcagg ccttcaatcc
     2761 ggaaaagcac gtcggcggcg gaatgccccg ggacataagt ccatgtgatg gt
//
LOCUS       DAOPPA010000435         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_38910_length_2810_cov_3.977858, whole genome shotgun sequence.
ACCESSION   DAOPPA010000435 DAOPPA010000000
VERSION     DAOPPA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38910_length_2810_cov_3.977858"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKH54_11665"
     CDS             <1..981
                     /locus_tag="PKH54_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00594.1"
                     /translation="RLEDAIKARNSAGVLTILSQYLELPGVPSDLLATLYERALATAD
                     RLSSIEKMLGIGVRFWKAVRGGSGVRILRVMRPAAMKVLSSYDERAAWVAALEAVLDT
                     IPNDETRKMLAEWYLAQKLNQGDDPRGMAASREHDMVVLTCGRLLSGRLGPPFDDAVR
                     AHLRGLVLAGKPFDARHATAQQLTSRIELISEWCEGDAIADRMIVDWFNLWVCSKGGF
                     DAAEYDEMADAAFVAINHVSDAISRRDIAETALDILKVRLDSCRDDRGTSEAVLTRIS
                     ALSAYVPEASDLVETHREEQKDSKLLFYASVLGGAGIIAVVILLLNFFAC"
     gene            1022..>2810
                     /locus_tag="PKH54_11670"
     CDS             1022..>2810
                     /locus_tag="PKH54_11670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD00595.1"
                     /translation="MKSVIVSGVAFSLLFAACGETKMKDEKSGASEVTVNPVKNPAIE
                     TFDLPNGLKVFMVVDKSAPLVAVDINYHVGSRNERPGKTGFAHLFEHMMFQGSKHYDD
                     DYFKPLQDVGGQVNGATSTDRTRYWEIVPSAWLERALWLESDRMAYLLDAMTQERLDN
                     QKSVVSNERRQNYDNRPYGTVWEKMAAALYPPQHPYSWLTIGSLADINAATREDVAEF
                     FKTWYTPNNATLAIVGDIDIQATKELVTKYFGAIPPGPAVSAVGKWPVRLESDIVLDI
                     KDRVQLPRTYITWPTVGFFDADEAALDVFGMVFGGGRTSRLYQDLVYTRQIAQDAMAG
                     QRSSQIAGVFDIMLTPRPGVDPLELEKAALEILDEMLKNGITEDEMSRARTSIASSFI
                     RDMENIGGFGGLADRINAYQHMLGRPDGFAADLQRYLDLDRGDVTRVAARYLSGARVI
                     ARVLPAGENAPTGPDAADRTALPGKGTDRAFDLPDRQVFELENGLQVVLVQQSRVPLV
                     GFDLIVPGGNGLDPSGREGLASLTAAMLSEGCADMDSETFAKRIEALGASLNADADVD
                     CWFVGGSAVTTTFRDLLDLAFKMVSAPTMD"
BASE COUNT          526 a          830 c          877 g          577 t
ORIGIN      
        1 cgacttgaag acgccatcaa ggctcgaaat tccgctggcg tgcttacgat actttcccag
       61 taccttgaac tgccgggcgt cccttccgac cttctggcaa ccctttatga gcgggcgctt
      121 gcaaccgccg atcgcctttc gtcgatcgag aagatgctgg gcatcggcgt ccggttctgg
      181 aaggctgtcc gtggcggttc cggcgttcgg attctgcggg tgatgcgccc ggccgcaatg
      241 aaggttcttt cgtcttatga tgaaagggcc gcatgggtcg ccgcgcttga agcggttctt
      301 gacacgattc cgaacgacga aaccaggaag atgctggccg aatggtacct tgcccagaag
      361 ttgaaccagg gcgacgaccc gcgcggcatg gccgcaagcc gggaacacga catggtcgtc
      421 cttacctgcg gacgccttct ttccggccgc ctgggaccgc cgtttgacga cgcggttcgc
      481 gcccatctgc gtgggctggt actggccggc aagccgttcg atgcgcgcca cgcgacggcc
      541 cagcagttga cttcccgcat cgagctcata agcgagtggt gcgaaggcga cgcgattgcg
      601 gatcggatga ttgttgactg gttcaacctc tgggtgtgct cgaagggcgg tttcgatgcg
      661 gccgaatacg acgaaatggc cgatgcggca ttcgttgcca tcaaccatgt gtccgacgcg
      721 atttccaggc gcgacatcgc ggaaaccgcc ctggatatcc tgaaggtccg gctggactcg
      781 tgtcgggatg accgcggtac gtccgaggcc gtgcttaccc ggatttcggc gctttccgcc
      841 tatgttcccg aagcatctga cctggtcgag acgcatcgag aggagcagaa ggattcaaag
      901 ctgctgtttt atgcatcggt tcttggcggc gccggcatca tcgccgtcgt gatcctgctg
      961 cttaactttt tcgcctgttg acacgtcggc gcaggccggc aacttctgct gaaggaacat
     1021 tatgaagtca gtaatcgttt caggagtcgc cttttcgctg cttttcgcgg catgcgggga
     1081 aacaaaaatg aaggatgaaa aatccggcgc ctcggaagtc accgttaatc cggtgaagaa
     1141 tccggccatt gaaacgttcg atctgccgaa tggcctgaag gtgttcatgg tcgttgacaa
     1201 gtcggcgccg ctggtcgccg tcgatatcaa ctaccacgtc ggttcccgga acgaacgtcc
     1261 cggcaagacc gggttcgcgc accttttcga acacatgatg ttccagggat caaaacatta
     1321 cgacgacgat tacttcaagc ccctgcagga cgtgggcggc caggtcaacg gcgcgaccag
     1381 caccgaccgg acccgttact gggaaatcgt cccgtcggca tggctcgagc gggcgttgtg
     1441 gcttgagtcc gaccgcatgg cgtacctgct cgacgcgatg acccaggaac ggctggacaa
     1501 ccagaagtca gtagtttcga acgagcgtcg ccagaactat gacaacaggc cgtacgggac
     1561 ggtgtgggaa aagatggcgg cagcccttta cccgccccag cacccatatt cctggcttac
     1621 gatcggttcg ctcgccgata tcaacgcagc gacccgcgag gacgtggccg agttcttcaa
     1681 gacctggtac acgccgaaca acgcaacgct cgccatcgtc ggcgacattg acattcaggc
     1741 cacgaaggag cttgtgacga agtatttcgg cgcgattcca cccgggccgg ccgtttcggc
     1801 ggttggtaag tggccggttc gccttgagtc cgacatcgtc ctggatatca aggatcgcgt
     1861 gcagcttccg cgtacttaca tcacatggcc gacggtcggg ttctttgatg ccgacgaggc
     1921 cgccctggac gtgttcggga tggtttttgg cggtggccgg acgtcgcggc tttaccagga
     1981 tctggtctat acgcggcaga tcgcccagga cgccatggcg ggccagcgca gcagccagat
     2041 cgccggcgtt ttcgacatca tgctgacccc gcgtccgggc gtggatccgc ttgaactcga
     2101 aaaggcggcg cttgaaatcc tggacgaaat gctgaaaaac ggcatcaccg aggacgagat
     2161 gtcccgcgcc aggacttcaa ttgcttcatc cttcatacgc gacatggaaa atatcggcgg
     2221 ttttggcggc ctggccgacc ggatcaacgc ctatcagcac atgctcggca ggccggacgg
     2281 ttttgcggcc gacctgcaga ggtaccttga ccttgacagg ggcgacgtaa ccagggttgc
     2341 cgcccgttac ctgtcgggcg ccagggtgat tgcccgcgtg cttcccgccg gggagaacgc
     2401 gcccaccggc cccgatgccg ccgatcgcac ggcgcttcct ggaaagggaa ccgatcgtgc
     2461 tttcgatctg ccggatcgcc aggtttttga gctggagaac gggcttcagg tcgtgctggt
     2521 tcagcagtcc cgcgtgccgc ttgttggatt cgatctgatc gttccaggcg gcaacggtct
     2581 tgatccgtcc ggcagggaag ggctggcgtc gcttacggcg gccatgctca gcgaaggatg
     2641 cgcggacatg gactccgaga ccttcgcgaa gcggatcgag gccctgggcg cgtcccttaa
     2701 cgcggatgcg gacgtggatt gctggttcgt cggcgggtcc gccgtcacga cgactttccg
     2761 ggatctgctc gatctggcct tcaagatggt ttccgccccg acaatggatc
//
LOCUS       DAOPPA010000436         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39087_length_2800_cov_4.116940, whole genome shotgun sequence.
ACCESSION   DAOPPA010000436 DAOPPA010000000
VERSION     DAOPPA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39087_length_2800_cov_4.116940"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1109
                     /locus_tag="PKH54_11675"
     CDS             69..1109
                     /locus_tag="PKH54_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD00596.1"
                     /translation="MNLPNPTQATRFPGRVTLVTGAGGSIGSELCRQVAAMGPSRLVL
                     LDIDETEIFLLADEISWLFPEVPTTCIVGDVRDNSRIGRIFETLRPDVVIHAAAYKHV
                     PMMEDNAWEAVGNNVRGTMCVASEAGRHGAAAFVNLSTDKAFRPCSIMGATKRVAELA
                     VRICADEYPDTSYSSVRFGNVRDSRGSVIPTFRRQIERRVPLTVTHPEATRYFIDIPD
                     AARLVLVAATTGAHGDIFTLDMGEPVRIADLASEMIRESGLNLEIVYTGLRPGEKLTE
                     ENPIEEKDCIFTGIPGLYVNRARLIDAGAFKSGLNALLRLPASSEAHEIRCILASMDI
                     GLQQGFCQSNGE"
     gene            1110..2411
                     /locus_tag="PKH54_11680"
     CDS             1110..2411
                     /locus_tag="PKH54_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD00597.1"
                     /translation="MNLIDRIHDRTAVIGILGLGYVGLPLADAFIRKGFTVIGLDTDD
                     SKVRTLKQGKTYIKHIPDDKITGFLKAGFTPTSDFSMVAKCDAVLVCVPTPLNQNLEP
                     DMTFVIGSCRDIAANMRPGQMIILESTTYPGTTVEVMKPVLEEGSGLKADRDFWLAYS
                     PEREDPNNSEYECETIPKVVGADCEYAREAAVALYGQVIRRVVPVSSTRVAEAAKILE
                     NTFRAVNIALVNELKVVFTRMGIDVWEAIDAAATKPFGFMKFTPGPGIGGHCIPIDPF
                     YLTWKAREYEVYSRFIETAGQVNRAMPAYVVSRTMEAFSQRGRGLAGSTILIVGLAYK
                     KNMDDARESPSYRLMDLLEQQGVTVSYHDPHIPVIPPSREYSRFMGRKSVDPATAGDF
                     DAVLISTEHDDVDWDDLLQRARLIIDTRGVFRQPSEKVVKA"
     gene            2431..>2800
                     /locus_tag="PKH54_11685"
     CDS             2431..>2800
                     /locus_tag="PKH54_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00598.1"
                     /translation="MDKPYFAHESAFIDGETPDNGRVDPNIGRGARIWHGCHVMAGAR
                     IGDGTSLGQNVFVGSAAIIGNGCRIQNNVSIYDGVTLKDLVFCGPSMTFTNLSWPLPR
                     AGIIRHSAFQKTCVDRGASIG"
BASE COUNT          590 a          835 c          838 g          537 t
ORIGIN      
        1 tggcgatcta cgagtcggcg cggacgggga tgacggttcg gctttgatct tccgggggca
       61 catatcaggt gaatcttcca aatccaacgc aagccaccag gtttccaggc agggtgactc
      121 tggtgacggg cgccgggggt tcgattggat ccgagctttg ccggcaggtc gccgcgatgg
      181 gcccgtccag gctggtactc cttgacatcg acgaaaccga gatcttcctg cttgccgacg
      241 aaatttcctg gctcttcccc gaggttccga ccacctgtat tgttggtgac gtgcgcgaca
      301 actcacggat tggccgcatc ttcgagactt tgaggccaga cgtggtaatc catgcggccg
      361 cctacaagca cgtcccgatg atggaagaca acgcctggga ggccgtcgga aacaacgtac
      421 gcggaaccat gtgcgtcgca tccgaggccg gcaggcacgg ggccgccgca ttcgtgaacc
      481 tttcgaccga caaggccttc cggccgtgtt cgattatggg tgcgaccaag cgcgtggctg
      541 aactggctgt ccggatttgt gccgacgagt atcccgatac cagctattcc agcgtccgct
      601 tcggaaacgt ccgcgattcc cgcggttcgg tcattccgac attcaggcgg cagatagagc
      661 ggagggttcc gctgacggta acgcatcccg aagccactcg ttacttcatc gacattccgg
      721 acgctgccag gctggttctt gtcgctgcaa cgaccggggc gcacggcgac atattcacgc
      781 tcgacatggg cgagcccgtc aggattgccg acctggcctc cgaaatgatt cgcgaatccg
      841 ggctgaacct ggaaatcgtc tatacaggac tccggcccgg ggagaagctg accgaggaga
      901 acccgatcga agagaaggac tgcattttca ccgggattcc cggcctttac gtcaaccggg
      961 cccggctgat cgatgccggg gcattcaaat cgggcctgaa cgcgctgctt cggctgccgg
     1021 cgtcttccga agcgcatgaa atcaggtgta tccttgcctc gatggacatc ggtcttcagc
     1081 aagggttctg ccagagcaac ggagaatgaa tgaacctcat tgacaggata cacgacagga
     1141 cggccgtcat tggcatactc ggactgggct acgtcggact cccgctggcg gacgcattca
     1201 tccggaaggg attcacggta atcggactgg atacggatga ttcgaaggtt cgcacgctga
     1261 aacagggcaa aacttatatc aagcatattc ccgatgataa aatcaccggc ttcctcaaag
     1321 ccggattcac gccgacgtcc gacttctcca tggtcgcgaa gtgcgacgcc gtccttgtct
     1381 gcgttcccac cccgctcaac cagaatctcg agcctgatat gacattcgtc atcggctcat
     1441 gtcgtgacat cgcggccaac atgcgtcccg gccagatgat catcctggaa tcgaccacct
     1501 atccggggac gacggtcgag gtcatgaaac ctgtcctgga agagggaagc ggactgaagg
     1561 ccgaccggga cttctggctt gcctactccc cggagaggga agatccgaac aacagcgaat
     1621 acgaatgcga aaccatcccg aaagtggtcg gggccgactg cgaatacgcc cgggaagctg
     1681 cggtggctct ttacggccag gtgatccgtc gcgtcgtgcc cgtcagttcg acccgagtgg
     1741 ccgaggcggc gaagatactg gaaaacacct tccgtgccgt caacattgcg ctggtcaacg
     1801 aactgaaggt agttttcacg cgcatgggca tcgacgtgtg ggaggcaatc gatgcggcgg
     1861 caaccaagcc gttcggcttc atgaagttca cgccgggacc ggggatcggc ggacactgca
     1921 tcccgattga tcctttctac ctcacgtgga aggcacgcga atacgaagtc tattcgagat
     1981 tcatcgagac ggccggccag gtcaaccggg ccatgccggc atacgtcgtt tcccgcacca
     2041 tggaagcctt ttcccagcgt ggacgcggcc ttgccggttc gacgatactg atcgtcggac
     2101 ttgcctacaa aaagaacatg gacgatgccc gggaaagccc gagctaccgg ctgatggatc
     2161 tgctggaaca gcagggcgtg acggtcagct accacgaccc gcacatacct gtaatcccgc
     2221 cctcccgcga atactcgcgc ttcatgggac gcaagagcgt cgatcccgcg accgccgggg
     2281 atttcgatgc cgtactgatt tcgacggaac acgacgatgt tgactgggat gaccttcttc
     2341 aaagggcccg tctgatcatc gacaccaggg gtgtcttcag gcagccgtcc gaaaaagtcg
     2401 tcaaggcctg acccgactgg aggcaaagcg atggacaagc cttacttcgc ccatgaatcg
     2461 gccttcatcg acggggaaac ccccgacaac ggccgggttg atccgaacat cggccgcggc
     2521 gccaggatct ggcacggctg ccatgtgatg gccggggcgc gcatcggcga cggaacaagc
     2581 ctgggccaga acgtgtttgt cggttccgcg gccatcatcg gcaacggatg caggattcag
     2641 aacaacgtga gcatttacga cggagtcacg ctgaaggatc tggtcttctg cgggccgtca
     2701 atgactttca ccaacctttc gtggccgctt ccgcgtgccg gaatcatccg ccattcggcg
     2761 ttccagaaga cctgcgtcga ccgcggcgcg tcaattgggg
//
LOCUS       DAOPPA010000437         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39320_length_2788_cov_3.130260, whole genome shotgun sequence.
ACCESSION   DAOPPA010000437 DAOPPA010000000
VERSION     DAOPPA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39320_length_2788_cov_3.130260"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="amrB"
                     /locus_tag="PKH54_11690"
     CDS             complement(<1..1040)
                     /gene="amrB"
                     /locus_tag="PKH54_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD00599.1"
                     /translation="MIALLALLGSSGCDASPQAGPEIQPGFAGSFYTSDTAALRREVN
                     GYINAATAVDGLDAWAFISPHAGYVYSGPIAGHAYRQILAMKPTRVVVVAFTHRPFRN
                     DGTLRNKAIATTTAGAFKTPLGDLPVDLAEVGALLKEYPFITDDRALFDGEHSLEVQL
                     PFVQVAAPGAKIVPLMFGNQSDAEVVNQLAAALAKRYSGDRRTVVVVSTDMSHYFDYQ
                     TAVGLDRGNLKTVTDLDCDSMKRTMNTPREGFCGTALVLTLMTAQKALKWPGPVVLDY
                     RNSGDTAGDRRKVVGYGAVAFPRPGGSPKMTTNANAPGAGTGDLAHGARPHDNELTPQ
                     QKKTLLTIARQTV"
     gene            complement(1113..1334)
                     /locus_tag="PKH54_11695"
     CDS             complement(1113..1334)
                     /locus_tag="PKH54_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOD00600.1"
                     /translation="MKAPGHKEKDAACGADMGRRQFLKTALALLGLAGAGLARPFSPA
                     SGQTARQTGSDHLSAHPARYYVNGDELAG"
     gene            complement(1338..2318)
                     /gene="amrS"
                     /locus_tag="PKH54_11700"
     CDS             complement(1338..2318)
                     /gene="amrS"
                     /locus_tag="PKH54_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD00601.1"
                     /translation="MTDTVECRLCPRKCRLSDGGRGNCRVRMNRDGRLVSLVYGKPVS
                     LNIDPVEKKPLFHFLPGTKTFSLATAGCNLHCMNCQNWQISQLDPEDAETVDMSPAQI
                     VAAARQRGCQSISSTYTEPIVFFEYATDIATAAKAVGLKNVWVTAGFIEQEPLKEACR
                     VVDAANVDIKAFSDDFYRRICQGRLQPVLDAIETMCRQGVLVEITNLVIPGENDDMGM
                     IRDMCRFIVDKCGPTTPLHFSRFHPMYRMQERLPTPPETLFKAADVAVAAGLKHVYVG
                     NTDAGDHAHTWCPSCGRKVIERRGYQVLSNKVGSDGKCDFCGKPVAGIWG"
     gene            complement(2315..2542)
                     /locus_tag="PKH54_11705"
     CDS             complement(2315..2542)
                     /locus_tag="PKH54_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00602.1"
                     /translation="MTYCRAVILVAVLVGLVVGALLLKDDIAGLLSKDAPATGTPAAV
                     VGSPGPSTDRNAQERLSDHPARFWDAVGEGK"
BASE COUNT          503 a          857 c          854 g          574 t
ORIGIN      
        1 accgtctggc gggcgatggt cagcagggtc tttttctgct gcggcgtcag ttcgttgtca
       61 tgcggccgcg ccccgtgggc caggtcgccc gtgccggcgc cgggcgcgtt tgcgttcgtt
      121 gtcatctttg gacttccccc cggcctcggg aatgccaccg cgccgtaacc gacaaccttg
      181 cgccggtcgc cggccgtgtc accgctgttc ctgtaatcaa gcacgaccgg accgggccac
      241 ttcagcgcct tctgggccgt catcagggtc agcaccagcg ccgttccgca gaagccttcg
      301 cgtggcgtgt tcatggttcg cttcatgctg tcgcagtcca ggtcggtcac cgtcttcagg
      361 ttgccgcgat ccaggccgac cgccgtctgg tagtcgaagt aatgcgacat gtccgtcgaa
      421 acgacgacaa ccgtacgccg atccccggag tatcgctttg caagggcggc ggcaagctgg
      481 ttgacgactt cggcgtcgga ctggtttccg aacatcaggg gcacgatttt cgcccccggc
      541 gcggcgactt gaacgaaggg caactgcact tccagtgagt gttcgccgtc aaaaagcgcg
      601 cgatcgtcgg tgatgaaagg gtattccttc agaagggcgc cgacttcggc cagatcgacc
      661 ggcaagtcgc ccagtggcgt cttgaaggcc ccggcggtcg tcgttgcgat tgccttgttg
      721 cgaagggtgc cgtcattcct gaatggacgg tgtgtgaacg cgaccacgac gacccgcgtc
      781 ggcttcatcg ccaggatctg acgataggca tggcccgcaa tcggtcccga atagacgtac
      841 ccggcgtggg gcgagatgaa ggcccaggcg tcaaggccat cgacggccgt cgcggcattg
      901 atgtatccgt tgacttcgcg ccgcagggcc gccgtgtcgg aagtgtaaaa ggatcccgcg
      961 aatccgggct ggatttccgg tccggcctgc ggggaggcgt cgcaccccga agaacccaga
     1021 agagcaagca gcgcgatcat tgcccccacc ccctttgccg gatgaaatat gacgaaaccg
     1081 tccatgatcc cttcccgtta aggtcttctg gctcagccgg ccagttcatc gccattaacg
     1141 tagtaacgcg ccgggtgtgc cgacaggtga tcgcttccgg tctgccgggc ggtctgtccg
     1201 gaagctggcg aaaacggtcg cgcaagtccg gcgccggcaa ggcccagaag tgccagcgcg
     1261 gtcttcagga actgccggcg tcccatgtcc gccccgcaag ccgcgtcctt ttccttatgc
     1321 ccgggcgctt tcaacgctta cccccagatt cctgcaaccg gtttgccgca gaagtcgcac
     1381 tttccgtcac tgccgacctt gtttgataga acctgatagc cacgtctctc gattaccttg
     1441 cgaccgcatg acgggcacca cgtatgggcg tgatccccgg catcggtatt gccgacatag
     1501 acatgcttca gcccggcggc cacggcgacg tcggccgcct tgaaaagggt ttccggcggc
     1561 gtcggaaggc gttcctgcat tcgatacatg ggatggaacc ttgaaaagtg caggggtgtg
     1621 gtcggcccgc acttatcgac gatgaaccgg cacatgtccc gtatcatgcc catgtcgtcg
     1681 ttttcacccg gaatgaccag gttggtgatt tcgaccagga cgccctgcct gcacatggtt
     1741 tcgatcgcgt ccagaaccgg ctgaagccgt ccctggcata tgcggcgata gaagtcgtcc
     1801 gaaaacgcct tgatatcgac gttcgcggca tcgacgacgc ggcatgcctc tttcagtggt
     1861 tcctgttcga tgaacccggc cgtcacccat acattcttca gtccgacggc cttcgccgcg
     1921 gtcgcgatat ctgtcgcgta ttcgaaaaag acgatcggtt ccgtgtatgt cgaggagatc
     1981 gactgacatc ctcgctgcct ggccgcggcg acaatctgcg cgggcgacat gtcaacggtt
     2041 tcggcgtcct cgggatcaag ctggcttatc tgccagttct ggcagttcat gcagtgaagg
     2101 ttgcagcccg ccgtcgcaag cgagaaggtt tttgtgccgg gaagaaaatg aaacaggggc
     2161 tttttttcga ccggatcgat gttgaggctg accggctttc catagacaag cgagacaagg
     2221 cgaccgtcgc ggttcatgcg cacccggcag tttccccttc cgccgtccga caggcggcat
     2281 ttgcgggggc agaggcggca ttcaacggta tccgtcattt tccttccccg acggcgtccc
     2341 agaagcgggc ggggtggtcg gaaagtcgtt cctgggcatt gcgatccgtc gaaggccccg
     2401 gtgaccccac gactgccgcc ggggtgcctg tcgccggagc gtctttcgac agaagtccgg
     2461 cgatgtcgtc cttcagcaga agcgcgccga ccaccaggcc aaccagcacg gcaaccagga
     2521 tgacggccct gcagtatgtc atgtttcctt cgcagtccat tcggacattt tagaatccga
     2581 aaccccgatg tcaagccggt tcggatctga gttatcccct tataggaaag ggggtatgcc
     2641 cggggattgg tggcgggctg cgaccagatc gagtgcagcc gcggactcca aatacccgtg
     2701 tctctccatg aggggatcgc tcaggacttg tagcgctcga tccgaccatt tgaatggtta
     2761 tatgaaagac agggacgatt tcctggcg
//
LOCUS       DAOPPA010000438         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39388_length_2784_cov_3.213631, whole genome shotgun sequence.
ACCESSION   DAOPPA010000438 DAOPPA010000000
VERSION     DAOPPA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39388_length_2784_cov_3.213631"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PKH54_11710"
     CDS             complement(<1..1488)
                     /locus_tag="PKH54_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00603.1"
                     /translation="MRDNQFFLPIAVTVLLASPLLASGCGSSVGSDIVEADSGDVFDA
                     ALVDTFLTDTGIADADVGRDSSGEIVDATGETASDSVTDDSVQDVPDDVPDQGVDPGT
                     DEEVDPGKDVATDPGTDEETGEVDIIEIPQDEPSCLAAGYCWIGGNCRLPGALDPVRS
                     CHACRPDVSTADWTALVDDSPCDDGDVGTYDDKCVSGGCAGTFLNCPVVIGQCEQEAH
                     PDGTSNCNYAFPVGRACDDGDPCTCNDSCDGTGTCAGSPVTCLTVINPCFPNAHCDGS
                     CACALDPWPGPEKSCPITNGTGRCDGAGACELVSCSDGFVESIRSPGTCIRAFKSVSA
                     GESHTCAVTSDGTPFCWGDNSAGQLGDGSEMDRTSPRPVSGLGGVAGIVAGQDFSCAW
                     TTSGAAWCWGNNNVGQLGDGSAMPSNVPVPVSGMASGVTLMSAGSLHACALRNGDVWC
                     WGANGVGQVGVDSMSSNFLIPQKVAVSSPIFVDCGAQHTCAITFQN"
     gene            complement(1616..>2784)
                     /locus_tag="PKH54_11715"
     CDS             complement(1616..>2784)
                     /locus_tag="PKH54_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOD00604.1"
                     /translation="WQADPEYYPDLAGLIEGLHADGIRAFAYVNPFVMKNLPNHFDTM
                     EGDGMLVSEPSSGNTYEIDSPAGVAGVPDLTWPATAEYVQDQLGRIVSDLGFDGWMAD
                     FGEYFPMDARVWTDSNPGAYHNLFPVDWVRQSRDVMKKLRPEGDFVYFARSGWTGVQG
                     VAQIHWVGDQEATWSQFDGLPTVVPAMLNLGLSGQPYVTHDIAGFSGGPSTKELYLRW
                     TELGAFTPFMRTHDGNNRDDNWCWDRDYYTGEHFRRMAVIHDQIAADFFIPLAAASRQ
                     TGAPILRAMMLVFPEDRATWSISDQYMIGDKLLVAPILSPSNPDSVEEPDFDTATTAR
                     QVYLPAGDWYDVWNQDAAPIKGPQTITVNAPFGRPPVFSFGEPYAVLVGDEAVR"
BASE COUNT          600 a          902 c          775 g          507 t
ORIGIN      
        1 gttctggaaa gttatggcgc acgtgtgctg cgctccgcag tcaacaaata tcggacttga
       61 aaccgcgacc ttctgtggaa tcaggaaatt cgacgacatg gaatcgaccc cgacctggcc
      121 gactccgttg gcaccccagc accagacatc gccattcctg agcgcgcagg cgtgcaggga
      181 accggccgac atcagagtta cgccgcttgc catgccgctg actggaaccg gcacattgct
      241 cggcattgcc gaaccgtcac caagttgccc gacattgttg ttcccccagc accatgcggc
      301 gccggacgtc gtccaggcac atgaaaagtc ctgcccggca acgatgcccg cgactccgcc
      361 aagaccggaa accggcctgg gcgaggtcct gtccatttcc gaaccgtcgc caagctgccc
      421 tgccgagtta tcaccccagc aaaatggagt tccatcgctt gtaaccgcac atgtatgaga
      481 ttcaccggcg gaaaccgact taaaagcccg tatgcaggtt cccggggaac gtatggactc
      541 aacgaaacca tctgaacagg ataccagttc acatgcgcca gccccgtcac aacgaccggt
      601 gccgttcgtt atcgggcagg atttctccgg gcctggccag ggatcgaggg cgcaggcaca
      661 gcttccatcg caatgcgcgt ttggaaagca cggattgatg acggtcaggc aagtcaccgg
      721 cgacccggca caagtccccg tgccgtcaca ggaatcgtta caggtgcagg gatccccgtc
      781 gtcacaggct ctgccgaccg gaaaggcata attgcagttc gatgtgccgt ccggatgggc
      841 ttcctgttcg cactggccaa tcacgaccgg acaattcagg aatgtcccgg cacacccacc
      901 cgaaacgcac ttgtcatcat aggtgccgac atcgccatcg tcacacggcg aatcatcgac
      961 cagggctgtc cagtcagcgg ttgaaacgtc aggtcggcag gcatgacagc ttctgaccgg
     1021 atcaagggct cccggcaggc gacaatttcc gcctatccag cagtatcccg cggcaagaca
     1081 ggacggctcg tcctgcggaa tctcaataat gtcgacttca ccggtttctt catccgttcc
     1141 gggatcggtt gccacatcct ttcccgggtc gacttcttcg tccgttcccg ggtccacgcc
     1201 ttgatccgga acgtcatcgg gaacgtcctg aacagaatcg tctgtaaccg agtcggatgc
     1261 cgtttcaccg gtggcgtcga cgatttcgcc cgacgaatca cgcccgacat cggcatccgc
     1321 tattcccgta tccgtcagaa acgtatccac cagtgccgca tcgaagacgt cccccgaatc
     1381 cgcttcaacg atgtccgaac cgacgcttga accacacccg gatgccagaa gtggcgacgc
     1441 cagaagcacg gtcactgcaa ttggcaggaa aaactggtta tcacgcatga catgcctcgc
     1501 aacatgtgat gtacacatac taaaccacaa cggcacaagg aggcgccaga gaatcgacag
     1561 aaaacgacaa gcggcgacaa ttatggttcg accggccccg cgaacagttg acgattcacc
     1621 ggacagcctc gtcgccaacg agtacagcgt acggctcgcc gaacgaaaag accggaggac
     1681 ggccgaacgg cgcattcacc gtaatggtct gcgggccctt gatcggggcg gcatcctggt
     1741 tccacacgtc gtaccagtcg ccggccggca gatagacctg tcgggcggtg gtcgccgtgt
     1801 cgaaatcagg ctcttcgacc gaatccgggt tggacggact aaggatcggt gccaccagca
     1861 gtttgtcccc gatcatgtac tgatccgaaa tcgaccacgt cgcgcggtct tccgggaaaa
     1921 ccagcatcat cgcccgcagg atcggcgccc cggtctggcg tgaggccgcc gccagtggaa
     1981 tgaagaaatc ggccgcaatc tggtcgtgga tgacggccat gcgccggaaa tgctcgccgg
     2041 tatagtagtc cctgtcccag caccagttgt cgtcgcggtt gttgccgtcg tgcgtccgca
     2101 tgaacggggt gaacgcccca agctcggtcc accgcaggta aagctccttc gtgcttggcc
     2161 ccccgctgaa accggctatg tcgtgcgtga cgtaaggctg gccggacagt cccaggttca
     2221 gcattgccgg aaccacggtg ggcaggccgt cgaactgact ccacgtggcc tcctggtcgc
     2281 ccacccagtg gatctgagcg accccctgga cgccggtcca cccggatcgc gcgaaataga
     2341 caaaatcacc ctccggacgc agtttcttca tcacgtcgcg gctctggcgc acccaatcga
     2401 ccgggaacag gttgtgatac gccccggggt tggaatcggt ccacacccga gcatccatcg
     2461 ggaagtactc gccgaaatcg gccatccagc cgtcgaatcc cagatcgctg acaatccggc
     2521 caagctgatc ctgcacatac tccgcggtgg ccggccacgt caggtccggc acgccggcaa
     2581 cgccggcagg actgtctatc tcgtacgtgt tgccgctgga tggttccgac accagcatgc
     2641 cgtcgccttc catggtgtcg aaatgattcg gcaggttctt catcacgaac ggattcacat
     2701 atgcaaaagc cctgatccca tcggcatgaa ggccttctat caggccggcc agatccgggt
     2761 agtattcagg atccgcctgc cagc
//
LOCUS       DAOPPA010000439         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39481_length_2779_cov_2.851689, whole genome shotgun sequence.
ACCESSION   DAOPPA010000439 DAOPPA010000000
VERSION     DAOPPA010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39481_length_2779_cov_2.851689"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKH54_11720"
     CDS             <1..201
                     /locus_tag="PKH54_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00605.1"
                     /translation="GDSMASWMFNLKNWGDPNVIEIAVDENHQSSLALPLVPNVTVPT
                     ELSTDCNALRGQPCRPFQAIVP"
     gene            238..828
                     /locus_tag="PKH54_11725"
     CDS             238..828
                     /locus_tag="PKH54_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOD00606.1"
                     /translation="MKTNPNRDKHGPDFNALFDFALAQEGNFTTAQAAIAGYSPQLIA
                     KYLTNGRFIRVRRGIYRLVHFPAGEYEQLTAYWLWSFRQGVFSHQTALVLHDLSNIMP
                     NFASMTLPASWRSRRLRIPPGLLLYYSDVPPADTFLIGPLPVTSVSRTLQDCMQEHIA
                     PEFLFDALTQAIESGQVNRGQLPEIEEYLARFDHSR"
     gene            933..1826
                     /locus_tag="PKH54_11730"
     CDS             933..1826
                     /locus_tag="PKH54_11730"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD00607.1"
                     /translation="MESRPYATAASFKQALEYRLRQTTTGGAALTRYRQSLVFQRFLA
                     RVFSFFGDAAVLKGGVTMEIRISNARSTRDIDLQVVMKPLAATQVFQEIGRLDLNDFL
                     QYKIREIGPILTGLPESGGGVRLQAQAILAGKRYGDTFGVDVVFSNQNLLIADIVTAP
                     DYLLFAGIPAPELRLYPIEMHIAEKLHAYTMPRTRENSRIKDLPDIALLAGIREISRD
                     TFIEAVEQVFDSRKSHSLPKSLGSPPESWNLPYREMAMNDRLPWPDIGAVFLTVSSFL
                     NPVLHDPNKFRRWDPESWTWK"
     gene            complement(1831..>2779)
                     /locus_tag="PKH54_11735"
     CDS             complement(1831..>2779)
                     /locus_tag="PKH54_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00608.1"
                     /translation="NVLGFLTGDESDWAIYNKDTGFPNAANKAADTRAVWRLYPDLLT
                     YNGAMTNKHVGVFAGMADIQGIDFYNAACAPHITEFGVHPPLRGPYDYLRNARNNHMP
                     WLTWFYSQGTGSWDGHAAPHEIAIQAISVMAAGAKGLMWFMATQEGRDNLPDTWQAMS
                     DANHIFKGMREYLRQGDITGAAQSDPDTIVDMIRADGVLVVPVITAKAEVEMTDENCA
                     FWSLGLLEEEPHWILAERATDVTITVPDDFAVNDLFEVVAPSPENQSGIREITTPILV
                     QGRQITLVNVPLSNTEPTHVYVFATDSATRQAITDAMAE"
BASE COUNT          582 a          849 c          738 g          610 t
ORIGIN      
        1 ggcgacagca tggcgtcatg gatgttcaac ctgaagaact ggggcgaccc gaatgtcatc
       61 gagatcgcgg tcgatgaaaa tcaccagtcc agcctggccc tgcccctggt tcccaacgtc
      121 acggttccga ccgagctttc gaccgattgc aacgcccttc gaggccaacc ctgccgcccc
      181 ttccaggcca tcgtgccgtg acttgacgta aacttaaccc tgggttaagt tttgtgcatg
      241 aagactaatc caaacagaga taagcacggt cctgacttca acgccctttt cgacttcgcc
      301 ctggcccagg aaggtaactt caccacggcc caggctgcaa tcgccggata ctcgccgcaa
      361 ctgatagcta aatacctgac taacgggcga ttcatcagag tccgtcgtgg gatctaccgg
      421 cttgttcact ttccagccgg tgaatacgaa caactgacgg cctactggct gtggtccttc
      481 cggcagggtg tcttttcgca tcaaaccgcg ctggttcttc acgatctgtc gaacatcatg
      541 ccgaattttg ccagtatgac cctgccagcc agttggaggt cacgccggct tcgaattcca
      601 ccaggcctgt tgctttacta ttcagacgtc ccgccagcag acacctttct gatcggcccc
      661 ctgcccgtaa catctgtcag ccggacgctt caggactgca tgcaggaaca tattgccccg
      721 gaattcctgt tcgacgccct gacacaggca attgaatccg gccaagtcaa ccgcggtcag
      781 cttccggaga ttgaggaata cctggcccgc tttgatcaca gcaggtgact atccaagctc
      841 cagatccgca atgactcacc agaacgcatc caacctgtaa acaaaagcgc cccaatctcg
      901 aaatacccga ctcccaactt tgcgtgcata taatggaatc aagaccctac gcaaccgccg
      961 cctcattcaa acaggccctg gagtacagac tccgccagac caccactggt ggcgctgctc
     1021 ttactcgata ccgccagagt cttgttttcc agaggtttct cgctcgagtc ttctcattct
     1081 ttggcgatgc cgctgttctg aagggaggag taacgatgga gattagaatc tccaatgctc
     1141 gttccacccg cgacatagac ctgcaagtcg tcatgaagcc gttggcggca actcaggttt
     1201 tccaggaaat cgggagactc gatctcaatg acttccttca gtacaaaatc cgtgaaatcg
     1261 gccctattct gacaggactt ccggagtcag gcggaggagt tcgactgcaa gcacaggcga
     1321 ttcttgccgg caagcgatat ggcgacacgt tcggcgtcga tgtcgtgttc tcaaatcaaa
     1381 atctgctgat tgccgatatc gtgactgcac ctgactacct cctgttcgcc ggcattccgg
     1441 ccccggaact tcgcctatac cctatagaga tgcacatcgc agaaaagctt cacgcctaca
     1501 cgatgccacg cacaagagaa aactcgcgta tcaaggatct tcctgacatc gcgctactgg
     1561 ccggcattag agaaatttcg cgcgatacat ttatcgaagc tgtcgagcag gtcttcgatt
     1621 cacggaagtc ccactccctg ccaaagtcgc ttggatcacc accagaaagt tggaatctac
     1681 cctatcgcga aatggccatg aatgaccggc ttccctggcc tgacatcgga gctgtattcc
     1741 tgacagtctc gtcatttctg aatccagttt tacacgatcc gaataagttt cgcagatggg
     1801 atccggaatc atggacctgg aaatgaaagt tcactccgcc atcgcatcgg tgatggcctg
     1861 gcgggtggcg gagtcggtcg cgaacacata gacgtgggtg ggttcggtgt tggacagggg
     1921 cacgttcacc agcgtgatct ggcggccctg gaccaggatc ggcgtggtga tttcgcggat
     1981 tccggactgg ttttcagggg acggcgcgac cacttcgaac aggtcgttga ccgcgaagtc
     2041 gtccggcacg gtgatcgtga catccgtcgc gcgctcggcc aggatccagt gcggctcctc
     2101 ttccagcagc ccaagcgacc agaacgcaca attttcgtcg gtcatctcga cttcggcctt
     2161 tgccgttatc acggggacca ccagcacgcc gtccgcgcgg atcatatcca cgatggtgtc
     2221 gggatcggac tgcgccgctc ctgtgatgtc gccctggcgc agatactcgc gcatgccctt
     2281 gaagatgtga ttggcgtcgg acatggcctg ccatgtgtcc ggcaggttgt cgcgcccttc
     2341 ctgcgtcgcc atgaaccaca tcagcccctt tgccccggcc gccatgaccg atatcgcctg
     2401 gatcgcgatt tcatgcgggg ccgcgtgccc gtcccagctt ccggttccct ggctgtaaaa
     2461 ccatgtcagc cacggcatgt ggttgttgcg ggcgttccga aggtagtcgt aaggccctcg
     2521 cagcggcgga tgcacgccga attcggtgat gtgcggcgcg caggccgcgt tgtaaaagtc
     2581 gatcccctgg atgtccgcca tgcccgcgaa cacaccgaca tgcttgttgg tcatggcgcc
     2641 gttgtaggtc agcagatccg ggtacaggcg ccagacagcc ctggtgtcgg ccgccttgtt
     2701 cgcggcgttc gggaagccgg tatccttgtt atagattgcc cagtccgact cgtcgccggt
     2761 caggaaaccc agcacgttg
//
LOCUS       DAOPPA010000440         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39607_length_2771_cov_4.547865, whole genome shotgun sequence.
ACCESSION   DAOPPA010000440 DAOPPA010000000
VERSION     DAOPPA010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39607_length_2771_cov_4.547865"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKH54_11740"
     CDS             <1..1066
                     /locus_tag="PKH54_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOD00609.1"
                     /translation="MLDNVPGLRADAEAGKCAFGTIDTWLIHKLTGGVSHVTDPSNAC
                     RTLLFNIDRLQFDDALLGVLKIPRSLVPEVRPSAGSFGVTKGLDFLPDGIPITGVCGD
                     QQSALFGQTCFDEGQSKCTYGTGAFLLMNTGDKMVPSTHGLLTSVGWKVGDRTTYVLE
                     GSSFIAGAAVQWLRDSLGFIKSAPEVEELAASVPDSAGVVFVPALAGLGAPYWDPEAR
                     GGLFGLTRGATRAHIARAVLEGIALQIAELLDAMAKDLGKPLTELRVDGGASANNLMM
                     QFQADILDVPVMRPRNIETTALGAAMLAGIGIGMWDMNRLREMAAIDHRFGPQFVQSE
                     RDAYLNRYKSAVRCTCGHGC"
     gene            complement(1038..>2771)
                     /locus_tag="PKH54_11745"
     CDS             complement(1038..>2771)
                     /locus_tag="PKH54_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOD00610.1"
                     /translation="GAGDIVRVDNVIDWRSFATLAKATFPVTSGNPEATYDLGLGNIR
                     RGINTPDLFEVPAQQWADLTAGDNSFGVSIINDSKYGWDHPDEERLRLTLIHTPAGPT
                     FHHHTIDFGVNHTTWAIHGHGGDWRNGTPRQAARLNQPLAAFLSAAPHDGALGRKVGL
                     LTLEGPDADQVAVKAIKKDEDGRGLTIVRLQELHGRSAADVRIKAAGTISEAFIMDGM
                     EISAGSLEITDGQAVLGLAPYQIATIGLRIELDGASIRKATSRFVALPYNVDVVSTDE
                     NRADGAFDSTGASWSSNMWPANFTYSGVVFQLGPVVPGIPNAVKARGQKIDLEIGTDE
                     TLYMLAAADEDVPAAVFKVGDDAYTFPIQAWTGFIGQWDSRVVGDTIVETRDEMLPAY
                     LKQAPVALYGTHRHGPDGDHPYVFTYMFMHALKVQPGETSLVLPDDPRIKILAVTLSP
                     DASIATRLATSLFDMQDPIAAPGWHEPDPIEPVQPEVVEHACDIIQDTTGDTTGDATG
                     DITQPPDTVRPDATADASTDVPASSGGCSAGGSAAPDSFMPVLFVFLLGTVLSTRFRS
                     RAFSNRGRRCT"
BASE COUNT          480 a          836 c          899 g          556 t
ORIGIN      
        1 gatgctggac aacgtccccg gcctgcgcgc cgacgccgag gccggcaagt gcgcgttcgg
       61 gacgatcgat acatggttga ttcacaagct gaccggcggc gtttcccacg tcaccgatcc
      121 gtccaacgcg tgcaggaccc tgttattcaa catcgatcgc ctgcagtttg acgatgccct
      181 gcttggcgtc ctgaagatcc cgcgcagcct tgttccggaa gtacgcccgt cggccggcag
      241 cttcggcgtt acgaagggcc tggacttcct gcctgacggc atcccgatta ccggtgtctg
      301 cggcgatcag cagtcggccc tgttcggcca gacgtgtttt gacgagggtc agtccaagtg
      361 cacgtacggc accggggcgt tcctgctgat gaacaccggt gacaagatgg ttccgtcaac
      421 gcacgggctg ctgacgtcgg tcgggtggaa ggtcggcgac cggacgacgt atgtgcttga
      481 agggtcttcg ttcatcgccg gagccgcggt tcagtggctt cgcgattccc tcggtttcat
      541 caagtcggcg ccggaagtcg aggaacttgc ggccagcgtt cctgattcgg ccggcgttgt
      601 gttcgtcccg gccctggccg gtcttggtgc accatactgg gatcccgagg cccgcggcgg
      661 tcttttcggc ctgacccgtg gcgcgaccag ggcccacatc gcccgggcgg tgcttgaagg
      721 cattgccctg cagatcgcgg aattgctgga tgcgatggca aaggatcttg gcaagcctct
      781 gaccgaattg cgcgtcgatg gcggcgcaag cgcgaacaac ctgatgatgc agttccaggc
      841 cgacatcctg gacgtcccag tcatgcgtcc gaggaacatc gaaacgaccg ccctgggtgc
      901 cgccatgctc gccggcatcg gtatcgggat gtgggacatg aaccgcctgc gcgagatggc
      961 ggcgattgat caccgtttcg gcccgcagtt cgtgcagtcc gaacgcgacg catacctgaa
     1021 tcgttacaag tccgcggtca ggtgcacctg cggccacggt tgctgaacgc tcttgatctg
     1081 aatcttgtcg aaagaaccgt cccgagaaga aaaacgaaca atacaggcat aaatgagtcc
     1141 ggggcggcgg agccgcccgc gctgcagccg ccgctgcttg cgggaacgtc ggtggatgcg
     1201 tcggcagtgg cgtcgggccg aacggtgtcc gggggctgcg tgatgtcgcc ggtcgcatcg
     1261 ccggtcgtat cgccggtcgt atcctggatg atgtcgcacg cgtgctcgac cacttcaggc
     1321 tggactggtt cgatcgggtc gggttcgtgc catccaggcg cggcgatcgg atcctgcatg
     1381 tcgaaaagcg atgtcgcaag gcgtgtggcg atggacgcat ccggcgacag ggtcactgca
     1441 agaatcttga ttcgcgggtc gtcgggaagc acaaggcttg tttcgcccgg ctggaccttc
     1501 agcgcgtgca tgaacatata ggtgaaaacg tacggatggt cgccatccgg gccgtggcgg
     1561 tgggttccat acagcgcgac cggagcctgc ttcaggtacg ccggaagcat ctcgtcgcgg
     1621 gtctcgacga ttgtgtcgcc gacgacgcgg ctgtcccact ggccgatgaa tccggtccag
     1681 gcctggattg ggaaagtgta tgcatcgtcc ccgaccttga aaaccgcggc gggaacatcc
     1741 tcgtcggcgg ccgcaagcat gtacagcgtt tcgtccgtac cgatttccag atcgattttc
     1801 tgtcccctgg ccttaaccgc gttcgggatg ccggggacaa cggggcccag ttggaaaacc
     1861 acgccgctgt aagtgaagtt cgccggccac atgttcgaag accagcttgc gcctgtcgaa
     1921 tcgaacgcgc cgtcagcccg gttttcatcg gtggaaacca catcgacgtt gtacggcagc
     1981 gcgacgaatc gtgaagtcgc cttccggatc gatgctccat caagctcaat ccgcagccca
     2041 atcgtggcaa tctggtacgg tgcaaggccc agcaccgctt gaccgtcagt gatctcaagg
     2101 ctgccggccg aaatctccat gccgtccatg atgaacgctt cggaaatggt cccggccgcc
     2161 tttatcctga catcggccgc cgagcgtccg tgaagttcct gcagtctgac tatcgtcagg
     2221 ccacgtccgt cttcgtcctt tttgatcgcc ttgacggcca cctggtcggc gtcgggccct
     2281 tcaagcgtca gaagaccaac cttcctgcca agcgctccgt catgcggggc ggcagacagg
     2341 aacgccgcca gtggctggtt caggcgcgcg gcctgccggg gcgttccgtt gcgccagtcg
     2401 ccgccgtgcc cgtgaatggc ccatgtggtg tggttgacgc cgaaatcgat ggtgtggtgg
     2461 tgaaaggtcg ggccggcagg ggtgtggata agcgtcaggc gcaggcgttc ctcgtccgga
     2521 tgatcccatc cgtacttgct gtcgttgatg atcgagacgc cgaaactgtt gtcgcccgcc
     2581 gtcaggtcgg cccactgctg cgcggggact tcgaacagat ccggtgtgtt gatgccgcgc
     2641 ctgatgttgc cgagtccaag gtcataggtc gcttccggat ttcctgatgt gacggggaag
     2701 gtggccttgg ccagcgtggc aaaggaacgc cagtcgatta cattatcgac gcggacgatg
     2761 tcgccggcac c
//
LOCUS       DAOPPA010000441         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39653_length_2769_cov_3.400147, whole genome shotgun sequence.
ACCESSION   DAOPPA010000441 DAOPPA010000000
VERSION     DAOPPA010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39653_length_2769_cov_3.400147"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKH54_11750"
     CDS             complement(<1..241)
                     /locus_tag="PKH54_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00611.1"
                     /translation="MKMQYFTAVAVIALVTACTSTVPEDDLVVHIDTSQPDNGQIEDG
                     AEPDSRPDLAEPDATTDIGDPDEGSATTVRVVGILE"
     gene            440..1150
                     /locus_tag="PKH54_11755"
     CDS             440..1150
                     /locus_tag="PKH54_11755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOD00612.1"
                     /translation="MVFKTVAVVVGAGMGRRVGTRRPKQFEEVSGRPLITYTLDIFDR
                     MAEIDGIIVVVPADWVDFTRAMVTRCQYSRIIDVIPGGASRQMSSFSGVILARRFGGP
                     HLKKIVVHDAVRPLASVELVQKVLKAADRTGAATAAARVTDTIFSISGDTVTGVVDRS
                     SLVAVQTPQAFDPELLVQAHRNALTAGITDASDDVGLVLRDGGSAVVVESDTGNLKIT
                     YGSDMRLLERLLETVKDE"
     gene            1143..2090
                     /locus_tag="PKH54_11760"
     CDS             1143..2090
                     /locus_tag="PKH54_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOD00613.1"
                     /translation="MSESFIGIDLGATRIKAGLVRDERILGTVVVNLDPADRSQSGVV
                     ARLAGVVRELGDRFLEPGQAVAGIGIGSPGAIRHAEGIIVRAPNFEQWNDFELGRMLG
                     EATGLPAKLDNDANVITLGEAVYGAGRGYRHFLCLTLGSGLGGGLFLDGRIYRGADGM
                     AGELGHIGVEPEGFECNCGSRGCVEQYIAAVGLRNYVRRDRLFGDGTEAALADPDLPR
                     RLYEMAKAGHPVAGRYFHDIGYYFAMAIGTYLNLLNVEAIVIGGGMARSWDAFGPTLM
                     SELHRRAYPVLVERTRIIMCELWEDAGILGAAALAGGRS"
     gene            2217..>2769
                     /locus_tag="PKH54_11765"
     CDS             2217..>2769
                     /locus_tag="PKH54_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOD00614.1"
                     /translation="MDKTGVRSRPKVLLARLFGPGTADVAAFLKGMKGADGERPLCWD
                     EGPEVVVKCVGPDVLEALSAAFGDSLYSIDGRRMHEVAAGLLAAGQWKLSTAESCTGG
                     LVSQLLTSVAGSSVFFEGGLVTYSNLSKSELLHVPAERIANSGAVSEDVARAMASGCR
                     SVLRTDLTVAISGIAGPSGGYPDK"
BASE COUNT          502 a          791 c          910 g          566 t
ORIGIN      
        1 cttcgaggat accgacgacc cggacggtcg tggcggaccc ttcgtcggga tcgccgatat
       61 cagtcgtggc atctggttcg gcaagatccg gacggctgtc cggttcagcc ccgtcctcga
      121 tctgaccgtt gtccggctgg cttgtgtcga tatgaaccac aagatcgtct tccggaacgg
      181 tcgacgtaca cgccgtaacc agtgcgatga cggcaactgc cgtgaaatac tgcattttca
      241 tgagcttggt atccccttcg aacccatgtg ccgtcaaaag ccatccgacc gacgaatctt
      301 aacggattac ggtcgattgc gaaacatcgt tattctaccc cgacctggga atcggattgt
      361 acttcgactt gggaaccggc ccaaacgctg ctattttata aggccgtggc tcattccttc
      421 tcggggcggg gaaacgacga tggttttcaa gacggttgcg gtcgtggtcg gggccggcat
      481 ggggcgcagg gtcggcacgc gtcggccgaa gcagttcgag gaagtttccg ggcgacccct
      541 gatcacctac acgctcgaca tttttgacag gatggccgag atagacggca tcatcgtggt
      601 cgtcccggcc gactgggtcg atttcacgcg ggcgatggtc acccgctgcc agtactcaag
      661 gattatcgac gtgataccgg gtggcgcctc acgccagatg tcgtcgtttt cgggagtcat
      721 ccttgcccgg cgtttcggcg gtccccacct gaagaagatt gtcgtgcacg acgcggtaag
      781 gccgcttgcg tcagtcgaac tggtccagaa ggtcctgaag gccgctgatc gaaccggggc
      841 ggccacagcc gcggcccggg taaccgacac gatattttcc atctcgggcg ataccgttac
      901 cggcgtcgtt gatcgttcat cccttgtggc cgtccagaca ccccaggcgt ttgaccccga
      961 gcttcttgtc caggcgcatc ggaacgcgct tactgcggga atcaccgacg cttccgacga
     1021 cgttggcctg gtgcttcgtg acggcggcag cgcagtggtg gtcgaatccg ataccggaaa
     1081 cctgaaaatc acttacggca gcgacatgcg tctgctggaa aggctgctgg agaccgtgaa
     1141 agatgagtga gtcctttatt ggcatagatc tgggcgcgac aaggatcaag gccggactcg
     1201 tccgggatga aaggattctt ggaaccgtgg tggtcaacct ggatcctgcc gacaggagcc
     1261 agtccggggt agttgccagg ctggcaggcg tggtcaggga actgggggat cggtttctgg
     1321 aacccggcca ggcggtcgcc ggaatcggga tcggttcgcc cggagccata cgtcatgcgg
     1381 aaggcatcat cgtccgcgcc ccgaattttg agcagtggaa cgatttcgaa cttgggcgga
     1441 tgctcgggga ggccaccggg cttccagcga agttggacaa cgacgcaaac gtcatcacgc
     1501 ttggcgaggc cgtttacggg gccggccgtg gatatcggca cttcctctgc ctgacgctgg
     1561 gaagcggact tggcggcggc ctgttcctgg acggacggat ttaccgtggc gccgacggca
     1621 tggccggtga acttggacat atcggcgtcg agcccgaggg attcgagtgc aactgcggca
     1681 gccgcggctg cgtcgagcag tacatcgccg cggtcggcct tcgcaactac gtaaggcggg
     1741 atcgcctttt cggcgacggc accgaagcgg cacttgccga ccccgacctg ccgcgacgtc
     1801 tttacgaaat ggcgaaggcc ggtcatccgg ttgccggccg gtacttccac gacatcggct
     1861 actacttcgc catggcaatc ggaacctatc tgaacctgct gaacgtggaa gccatcgtga
     1921 tcggtggcgg gatggccagg tcatgggacg cgttcggtcc gacgctgatg tccgaacttc
     1981 atcggcgtgc gtatccggtg ctggtcgagc ggacgcgaat catcatgtgc gagctttggg
     2041 aagacgccgg catacttgga gcggcggctc ttgccggagg gcgatcctga tcgccacgga
     2101 agccgggcga cccggcgaaa aacctttttt ctcccgttca aagcccccgt cccttgaacc
     2161 gtctatgtgg taaactgtcg ccgcagcccg ccttcgcgtg gccggatggg ggattgatgg
     2221 acaaaactgg tgtcagatcc aggccaaagg ttttgctggc gcggcttttc gggcccggca
     2281 ccgcagacgt ggccgctttc ctgaagggca tgaagggcgc cgacggcgaa aggccgctgt
     2341 gctgggacga ggggcccgag gtcgtcgtca agtgcgtcgg tcccgatgtg cttgaagcct
     2401 tgtcggccgc ttttggcgac agtctgtatt cgattgacgg cagaaggatg cacgaagtcg
     2461 cggcgggact gctcgcggcc ggtcagtgga agctatcgac cgcggaatcg tgcaccggag
     2521 ggctggtcag ccagttgctg acttccgttg ccggttcgtc cgttttcttc gagggcgggc
     2581 tggtgacgta ctcgaacctt tcgaagtcgg aactgctgca tgttcctgct gaacggatcg
     2641 ccaactcggg ggcggtttcc gaggatgtgg cccgcgcgat ggcatcggga tgcagaagtg
     2701 tactgagaac cgacctgacg gtcgcgattt cgggtattgc cggaccttcg gggggttacc
     2761 ctgacaagc
//
LOCUS       DAOPPA010000442         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39687_length_2767_cov_3.432891, whole genome shotgun sequence.
ACCESSION   DAOPPA010000442 DAOPPA010000000
VERSION     DAOPPA010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39687_length_2767_cov_3.432891"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKH54_11770"
     CDS             complement(<1..662)
                     /locus_tag="PKH54_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00615.1"
                     /translation="MAEKSPRVMVVTYGCQMNVFDSRRMIQVLGHAGYVETDDPAAAD
                     VIIFNTCSVRDRPEKKVLGSLSRLLPLKEIKPVLFGVAGCVAQQHGQALLDRVPYLDF
                     VIGPDNIADLPDVVTRATSGRRTSSATWMDPRSYDFIPLDADVEAGPVAFLTIIKGCD
                     NFCSYCMVPYVRGREVSKPAGLVLDEVRGLVKAGISEVTLLGQNVNSWGRGLEGNPTF
                     PDL"
     gene            complement(786..>2767)
                     /locus_tag="PKH54_11775"
     CDS             complement(786..>2767)
                     /locus_tag="PKH54_11775"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOD00616.1"
                     /translation="LLAVLGGVGAGWMAFSTTDYLLWAITFAVFLACLKSLYLRMAVD
                     FMHMYALSFLTVMAAAVVNPGLSFGIVMLPYTVLLVFGLMLNSLRQNMERSILDRGRP
                     DSAADAAAFMGRRGIIRRRFVLVTVGITLAVFFSNVLFFLLFPRMGLGFFARQQRQGV
                     AMTGFSEEVTLGDFGNIISDLDVVMRVVPNGGPEAGMLRLPLRMKGQSLDSYDGRTWR
                     KTTPRRYQLDTGEGGLLRPRRSRDEAPPGPSFERDVYLEPMPGTVRVLFGEPMITGFR
                     PPMGMLDAMRTDKLRFFADPAGDVTMTGPESTALVYTVESSPPPSDPALLRVAGDSWP
                     QRIKDLYLPVPPQDPGVSALARRIAAGRDNAFDIAVAMENELRNGWAYSLDSVHGNTD
                     PVADFLLRNKTGHCEYFASSMVIMLRVLGIPARIVNGFYGGERNEFGNYVALRKSDAH
                     SWVEVYFPGSGWAVFDPTPPGALDLRKGGGLLAEIRAAIDAVKLTWYQWVIEYDLEKQ
                     FEMVARLLKVKKDKGFGDNINRRDIRRIAREIRNLPWGTIFGIPVLLFGLFVGVRALA
                     PRVLKGRGLPSAVPAVIHYRRARRMLRRRGLARAGSETQIEFAARAGLEFPSVAADLE
                     LIAWQYCAVLAGRQPSVPPGELDAALERIRRALAS"
BASE COUNT          598 a          892 c          811 g          466 t
ORIGIN      
        1 agatcgggga aggtcggatt tccttcaagg ccccgccccc atgaattcac gttctgcccg
       61 agcagcgtca cctcggatat tccggccttg accaggcccc tgacttcgtc caggaccagt
      121 ccggccggct tgctgacctc gcgaccacga acgtaaggca ccatgcagta tgaacagaag
      181 ttgtcgcagc ccttgataat cgtaaggaaa gccaccgggc cggcctcaac gtcggcatca
      241 agcggaatga agtcgtagga tcgcggatcc atccatgtgg cggaagacgt ccttctgccg
      301 gaagtggcgc gggtgacgac atctggaaga tccgcgatat tgtcgggccc gatgacgaaa
      361 tccaggtatg gcaccctgtc caggagcgcc tgcccgtgct gctgggcgac gcacccggcg
      421 acgccgaaca ggaccggctt gatctccttt aacggcaaaa gccgtgaaag gctgccaaga
      481 accttctttt ccggcctgtc cctgacgctg cacgtattga aaatgatgac atcggcggca
      541 gcgggatcgt ctgtttccac atatccggca tggccaagaa cctggatcat ccgacgggaa
      601 tcgaacacgt tcatctgaca gccgtaggta acgaccatca cccgcggcga cttttccgcc
      661 aatccatcgt ccggccggca ctgcccggaa accgcggatt ccgccgcccg gccagtatta
      721 ctcgacaggt ctgtgggtgt accgtccatc atggcggcca agggtatccg tggcaacgct
      781 ccgcgtcaag acgccagggc acggcggata cgttcaaggg cggcgtccag ttccccgggc
      841 gggaccgaag gctgccgacc ggccagaacg gcgcagtact gccaggcgat cagttccaga
      901 tccgccgcca ccgacgggaa ctccaaccct gcccttgcgg caaactcgat ctgcgtctcg
      961 gagccggccc gggcaaggcc gcgtctgcgc aacatgcgcc ttgccctgcg gtaatggatt
     1021 acggccggca ccgcggatgg cagcccccgt cctttcaaca cgcgcggcgc aagggccctg
     1081 acaccgacaa acaggccgaa cagaagaacc gggatcccga aaatcgttcc ccatggcagg
     1141 ttccggatct cccgggcgat gcgacggatg tcccgacggt tgatattgtc gccgaacccc
     1201 ttgtccttct tgactttaag cagcctggca accatctcga actgcttttc caggtcgtat
     1261 tcgatgaccc actggtacca ggtcagcttg actgcatcga tcgccgccct gatctcggca
     1321 agcagcccgc cgcccttgcg aaggtcgagc gcacctgggg gtgtcgggtc aaaaacggcc
     1381 cagcccgatc ccgggaaata gacttccacc cacgagtggg cgtccgactt gcgaagcgcc
     1441 acgtagttgc cgaattcgtt gcgctcccca ccgtagaatc cgttgactat cctggccgga
     1501 attccaagga cacggagcat gatgaccatc gaggacgcaa aatactcgca atgaccggtc
     1561 ttgttccgaa gaaggaaatc ggccaccgga tcggtattcc cgtgaaccga atccagggaa
     1621 tatgcccacc cgttcctcaa ctcgttctcc atcgcgaccg caatgtcgaa cgcgttgtcc
     1681 cgacccgcgg caatcctgcg ggccagggcc gagacgccgg gatcctgggg cggaaccggc
     1741 agataaagat ccttgattcg ctgcggccag gaatcgccgg cgacacgaag aagggccgga
     1801 tccgacggtg gcggggatga ttccacggta tatacgaggg cggttgactc aggaccggtc
     1861 atcgtgacgt cgcccgcggg atccgcgaaa aagcgcaact tgtcggtgcg catggcgtca
     1921 agcatgccca tcggcggcct gaaccccgta atcatcggct ccccgaaaag cacgcgcacc
     1981 gtgccgggca tcggttcaag atatacgtcg cgctcgaagg aaggcccggg cggagcctcg
     2041 tcgcgggaac gccttggacg aagcaggccg ccttcgccgg tgtcgagctg ataccttcgc
     2101 ggagtcgtct tcctccaggt ccggccgtcg taactgtcaa gactctggcc cttcatccgc
     2161 aacggcagcc gcagcattcc ggcctcggga ccgccattcg ggacaaccct catcaccacg
     2221 tcgaggtcgc tgatgatatt tccgaaatcg ccaagggtga cttcctcgga aaagcctgtc
     2281 atggcaacgc cctgacgctg ctgcctggcg aaaaagccaa gtcccatcct tgggaacagc
     2341 aggaagaaca ggacgttcga aaaaaagacc gccagcgtga ttcccacggt caccagcacg
     2401 aacctgcgcc ggatgatccc gcgcctgccc atgaaggcgg cggcatccgc tgcgctgtct
     2461 gggcggccgc gatcaagtat cgaacgctcc atgttctggc gcaggctgtt caacatcagt
     2521 ccgaacacca gaagcaccgt gtatggcagc atcacgatgc cgaaggaaag gcccggattg
     2581 acgacggcgg cggccatgac agtaaggaac gaaagcgcgt acatgtgcat gaaatccacg
     2641 gccatccgca ggtacaggga cttcaggcag gcaagaaaga cggcgaacgt tatcgcccag
     2701 agaagatagt cggttgtcga aaaggccatc cagcccgcac cgacgccacc cagaacggcc
     2761 agtagcc
//
LOCUS       DAOPPA010000443         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39698_length_2766_cov_4.105865, whole genome shotgun sequence.
ACCESSION   DAOPPA010000443 DAOPPA010000000
VERSION     DAOPPA010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39698_length_2766_cov_4.105865"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKH54_11780"
     CDS             <1..827
                     /locus_tag="PKH54_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00617.1"
                     /translation="WLVESGRPAVAMTLATRAMAAGVIRPAVLAAASRLAADKGFDRP
                     ATAMFKLGLSSRQMNPSDWHAIAAVSLRRGDLKQAARAISGMGPEEGWGYDEIMIKGR
                     LLFRSKQYAQAIEQFSRAAALRPDSCQPLLYRGASALINGDAPAGEADFWTCLRLEPP
                     SPQVLGGLAFAMFDQGRYSESEGVFGRALNMEPENSDNLIGMAIAAFMAGDMDGARSA
                     HTYAVEYTPVFAKGPAAAEAAGYQYSRVQKDAWAKLRVWLDAEKKAAARAARGKRR"
     gene            877..2649
                     /locus_tag="PKH54_11785"
     CDS             877..2649
                     /locus_tag="PKH54_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOD00618.1"
                     /translation="MNFAARSIRFCLLSAMVLCLVTCSGRSGSSVGTGVTASVESSTV
                     QGADSAKSPITIRILSTNDIHGSLGSSMVDGAAGPGRLGGVEYLAGLIASLRQPAPER
                     TIVVDAGDCFQGDYAVNKSEGAFCTDFFNLVGYSVRTIGNHEFDYLDVGPDNEINDPR
                     GALKRMIANSRHPIVLANVREKAGDPLHPAIREYVILDVSGVRIGITGVLTPDTPSVS
                     RRAGSVGVEFEQPVDALRRVIPKMRSEGAGVVLVLSHLTGNCTDGRDLFATGAAACDV
                     GGELSRIQAAFTNQDIDLIVSGHDHVMLRGDGRSIPIVENPGHGIVVGNAEIDVDPAT
                     GRRVGAVRVLEHVPVCIPGEPVPKVCSSEWPGFRGVAVPNESVRQLRLSVEAAVASEC
                     ADIVATAATDITFSRTTEPPLANLAVDLLRELGTVVDSSGKAVLADFAFMNRGSTRAS
                     MAAGPVSVCDVARIWPFSDPPVLVSMTGAEIARMLTFLVDDVKKAPVISGMKVSRVAG
                     GPIEIRDAGGTVIDSNKQYRVVTTQYMVEGGDRLDEFFATFPAGRVHPLPFDSYRDGF
                     IKAIRARGVLNAPDTSRYSGITRD"
BASE COUNT          488 a          794 c          893 g          591 t
ORIGIN      
        1 tctggcttgt cgaaagcggc cgtccggccg tcgcgatgac tcttgcgaca cgggccatgg
       61 ctgccggcgt aatccggcca gcagtcctgg cggccgcttc aaggctggcg gccgacaagg
      121 gatttgatcg acctgcaacc gcgatgttca agctcggact gtcgtcgcgc cagatgaacc
      181 cgtccgactg gcatgctatc gcggccgttt cactgcgtcg cggcgacctg aagcaggcgg
      241 ccagggccat ttccggcatg gggcctgaag agggctgggg atacgacgaa ataatgatta
      301 agggccggct gcttttccgg tcgaagcagt atgcgcaggc gatcgagcaa ttctccaggg
      361 ccgcggcgct gcgcccggat tcgtgccagc ccttgctcta ccggggggca tcggctctta
      421 tcaacgggga cgcgccggcg ggcgaggcgg atttctggac atgcctgcgc ctggagcctc
      481 cctcgcccca ggttcttggc ggccttgcat tcgcgatgtt cgaccaggga cggtactctg
      541 aatccgaagg ggttttcgga cgagccctga acatggaacc ggaaaactcg gacaacctga
      601 tcgggatggc gattgccgcc ttcatggccg gcgacatgga cggtgccaga agcgcgcaca
      661 cgtatgccgt ggagtacacg ccggtctttg cgaagggccc tgcggccgcc gaggccgccg
      721 gctaccagta ttcaagggtt cagaaggacg cgtgggcaaa gctgcgtgtc tggcttgatg
      781 ccgaaaagaa ggccgccgcc agggcggccc gtggcaagcg tcgttgaacg ctttcgattt
      841 acttcatgtt ttccggtcat ttgcgcaggt gattgaatga attttgcagc ccgctcgatt
      901 cggttctgtc ttctgtccgc catggtcctt tgcctggtta cctgctccgg gcgatccggc
      961 agttcggtcg gaaccggggt gacggcgtcg gtcgagtcgt ccacggtaca gggcgccgat
     1021 tccgccaagt ccccaataac cataaggatt ctttcgacaa acgatataca cgggtcgctg
     1081 gggtcgtcga tggttgatgg cgcggccggt cccggtcggc tgggcggggt ggagtacctt
     1141 gccggattga tcgcttcctt gcgccagccg gcccccgaaa ggaccatcgt ggtcgatgcc
     1201 ggtgactgct ttcagggcga ctatgcggtg aacaagtcgg agggtgcttt ctgcaccgac
     1261 ttcttcaacc tggtgggata ttcggttcga accatcggaa atcatgaatt cgattacctt
     1321 gatgtcgggc ctgacaacga gatcaatgac ccgcgaggtg ctttgaagcg gatgatcgcg
     1381 aattcccggc atccaatcgt gcttgcaaat gttcgcgaaa aggcgggcga tccattgcac
     1441 ccggcgatac gcgagtacgt gattcttgat gtttccggtg ttcgcatcgg catcaccggt
     1501 gtcctgactc ccgatacgcc cagcgtttcc agacgtgccg gaagcgtcgg tgtcgagttt
     1561 gagcagccgg tcgatgccct tcgcagggtg attccgaaga tgcggtcgga aggggccggc
     1621 gtggtcctgg ttctgtcaca tttgaccggt aactgtaccg atgggcgtga tttgttcgcg
     1681 accggcgccg cggcctgcga tgtcggtggc gaactgtccc gcatacaggc ggctttcacg
     1741 aaccaggata tcgacctgat tgtttccggg catgaccatg tcatgcttcg cggcgacggc
     1801 cggtcgatac cgattgtgga gaaccccggc cacgggatcg ttgtgggaaa cgccgagatc
     1861 gacgttgatc ccgcaaccgg aaggcgcgtc ggggcggttc gggtgctgga acacgtgccg
     1921 gtttgcatcc ccggcgaacc cgttccgaag gtctgttcca gcgaatggcc cggatttcgg
     1981 ggggttgccg ttccaaacga gtccgttcgc cagcttcgtc tgtcggtcga ggcggccgtc
     2041 gccagtgagt gtgccgatat agttgcaacc gcagcaaccg atataacctt ctccaggacg
     2101 accgagccgc cgcttgcgaa ccttgcggtt gaccttctga gggaacttgg cacggtcgtg
     2161 gattcatccg gcaaggctgt tttggccgat ttcgccttca tgaacagggg gtcgacccgt
     2221 gcaagcatgg cggccgggcc agtttccgtc tgcgatgtcg cccggatctg gcctttttcg
     2281 gatccgcccg ttctggtgtc gatgaccggt gccgaaatcg cccgcatgct tacctttctg
     2341 gtcgatgacg tgaaaaaggc acccgtcatt tcgggcatga aggtttcccg ggttgcgggg
     2401 gggcccatcg agatcaggga tgccgggggc accgtaatcg attccaacaa acagtaccgg
     2461 gttgtcacga cgcagtacat ggtcgagggc ggcgacaggc tggacgagtt cttcgcgacc
     2521 ttcccggcgg ggcgcgtcca tccgctgcct tttgacagct atcgcgatgg attcatcaag
     2581 gcaatccggg cgcgcggcgt cctgaatgcg ccggatacat cgcggtacag cggtatcaca
     2641 agggattgaa atgatcctga aaattccaag gtgttttgat caccacaacc attacacgct
     2701 ttatctggcg ctttcgggct gtccaaccct tgacggcatc agtgaccgcg gggaagccct
     2761 tggact
//
LOCUS       DAOPPA010000444         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39792_length_2761_cov_4.103104, whole genome shotgun sequence.
ACCESSION   DAOPPA010000444 DAOPPA010000000
VERSION     DAOPPA010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39792_length_2761_cov_4.103104"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /gene="rplK"
                     /locus_tag="PKH54_11790"
     CDS             <1..334
                     /gene="rplK"
                     /locus_tag="PKH54_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOD00619.1"
                     /translation="ANIMEFCKAFNARTQKMEGSTVPVVITVFSDRSFTFVTKSPPTS
                     EQILKAAGLKKGSGRANRDKVGKITMAQIAEIAKNKMPDLNAKDEAGAIAIVAGTARS
                     MGVDVIES"
     gene            358..1065
                     /gene="rplA"
                     /locus_tag="PKH54_11795"
     CDS             358..1065
                     /gene="rplA"
                     /locus_tag="PKH54_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOD00620.1"
                     /translation="MPKHGKQYRALAANVDKTIKLSVDMAMDTVLKSGFAKFNESVDI
                     AVRLNVNPRHADQMVRGAVVLPAGSGKETRIVVFAKGEKEAEAREAGADFVGAEDLAE
                     KILGGWTDFDKAIATPNMMGIVGKLGKVLGPRGLMPNPKVGTVTMDVANAVREGKAGK
                     VEYKVDKNGVVHTFVGRRSFNADQLRQNFLAVMENVIKAKPSTVKGVYIKSITISTTM
                     GPGLRIDPQDVEATLKK"
     gene            1229..1762
                     /gene="rplJ"
                     /locus_tag="PKH54_11800"
     CDS             1229..1762
                     /gene="rplJ"
                     /locus_tag="PKH54_11800"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOD00621.1"
                     /translation="MNLEQKKGSVSELKDLMDSAKATVMLSQNGLTVGEVTEFRRALR
                     ANGAQLKVFKNTLLRRAIEGSSYEGIFDDVLKGPLAVAYTTGDAAGMAKAIVEFTKNR
                     KNITVLVGSLGDRKMEQSQLKALSELPPVEVLRASLLGALVAVPQKLLGVFQAPGRDM
                     VGVLAARERQLGGQADA"
     gene            1832..2227
                     /gene="rplL"
                     /locus_tag="PKH54_11805"
     CDS             1832..2227
                     /gene="rplL"
                     /locus_tag="PKH54_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOD00622.1"
                     /translation="MADITKEQVVEFLSGMTVLELAELTKELEAKWGVTAAAPVAMAA
                     MPMGGAAAAEEKPEEKTEFDVILKGFDADKKIGVIKEIRTVVAGLGLKEAKEFVEAAP
                     KTIKEGATKEEAEDIKKKLEAAGGQVEIK"
     gene            2389..>2761
                     /locus_tag="PKH54_11810"
     CDS             2389..>2761
                     /locus_tag="PKH54_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00623.1"
                     /translation="MSVTTKPKGLKTRCSFARIPEATQVPDLIRVQKESYERFLQTDT
                     PNDKRGDFGLHGIFKSIFPITDYNKTSSLEYKGYVLDEPKYDVEECRQRGITFASPMR
                     VTVQLVMYDVDPDTGVRDVRDI"
BASE COUNT          632 a          731 c          890 g          508 t
ORIGIN      
        1 tgcgaacatc atggagttct gcaaggcctt taacgccagg acccagaaga tggagggatc
       61 aacggtcccc gtggtaatca cggtgttttc ggaccgttcg ttcaccttcg tcaccaagtc
      121 ccccccgaca tccgagcaga tcctcaaggc tgccggactg aagaagggtt cgggtcgcgc
      181 gaaccgggac aaagttggga agattaccat ggcgcagatc gcggagatcg caaagaacaa
      241 gatgcctgac ctcaacgcaa aggacgaggc cggggccatc gcgatcgttg ccggtaccgc
      301 gcgcagcatg ggagtcgatg tcatcgaaag ttgacgttaa caaggcgagg tcgagagatg
      361 ccaaagcatg gaaaacagta tcgggcgctt gccgctaacg ttgacaagac catcaagctg
      421 tcggttgaca tggcgatgga cactgtcctg aagagcggtt tcgcgaagtt caacgagtcc
      481 gttgatatag ccgtaaggtt gaatgtcaat ccgcgccacg ccgaccagat ggttcgcggc
      541 gccgtcgtcc tgccggcagg ttccggcaag gaaaccagga tcgtcgtttt cgccaagggc
      601 gagaaggaag ccgaggcccg cgaagccggt gccgattttg tcggagccga ggacctggcc
      661 gagaagattc ttggtggctg gaccgatttc gacaaggcga tcgccacccc gaacatgatg
      721 ggaatcgtcg gtaagctcgg taaggtgctc ggtccccgcg gcctgatgcc caacccgaag
      781 gtcggaaccg tcacgatgga tgttgcgaat gccgttcgtg aaggcaaggc cggcaaggtc
      841 gagtacaagg ttgacaagaa cggcgtcgtc cacacgttcg tgggtcgtcg cagtttcaac
      901 gccgaccagc tccgccagaa cttcctggcg gtcatggaaa acgtgatcaa ggcgaagcct
      961 tcgaccgtga agggtgtcta catcaagtcg attacgattt cgacgacgat gggacccggc
     1021 ctgaggattg atccccagga cgtcgaggcg accctgaaga agtgatcttt ccggtcttcg
     1081 gaccggttgg tgtccgctgc ccgagaagtc ggttccgggg tcacccgggt aattccgacg
     1141 gaggcatgct cgttataagg acggtgtcaa tcgttcctct aacctgcctc gggtagctgc
     1201 agaaccggag tcaaaaggga gtaatcagat gaaccttgag cagaagaaag gaagtgtttc
     1261 ggaactcaag gacctcatgg actccgcgaa ggctacggtg atgctctccc agaacggcct
     1321 gacggtcgga gaggtaactg agttcaggcg cgccctccgg gcgaatggcg cccagctcaa
     1381 agtctttaag aataccctgt tgcggagggc catcgaaggc tcgtcgtacg aaggtatttt
     1441 tgatgacgtc ctcaagggac cgctggcggt ggcatacacg acgggcgacg cggccggaat
     1501 ggccaaggcg atcgtcgagt tcaccaagaa ccggaagaac atcaccgtgc tcgtcggaag
     1561 tcttggcgac aggaagatgg aacagagcca gttgaaggcc ctgtccgaac tgccgccggt
     1621 cgaggtcctg cgggcgtccc tgcttggtgc cctggtcgcg gtaccccaga agcttctggg
     1681 cgtgttccag gctcccggac gggacatggt tggcgtgctt gcggcgcgcg agcggcagct
     1741 cggcggtcag gcagacgcgt aggctggttg aaggaatttg tgccgcgaac gcctgaatgg
     1801 ccgcggccgg aaggaacaag gagatttgaa aatggcggac atcacgaagg aacaggttgt
     1861 tgaatttctc agcggtatga cggttctcga actggccgaa ctgaccaagg aactggaagc
     1921 gaagtggggc gttaccgccg ccgctccggt tgccatggcc gcgatgccga tgggcggcgc
     1981 tgccgctgcc gaggaaaagc ccgaggagaa gaccgagttt gacgtcatcc tcaagggttt
     2041 cgatgccgac aagaagatcg gcgtcatcaa ggaaatccgc accgtcgtcg ccggtctggg
     2101 cctgaaggaa gcgaaggaat tcgtcgaagc ggccccgaag acgatcaagg aaggcgccac
     2161 gaaggaagag gccgaggata tcaagaagaa gcttgaagcg gccggcggtc aggtcgagat
     2221 taagtaagtc gtttcgtttt gcacgagtga aacggtcggc actcctttgg ggctacccaa
     2281 aggagtgtcc ggccttttgc cgtatgaccg taaggctgtg cggcatgcgg tgcgacaccc
     2341 ggtcgtgtcc gcgttgatcg ttaaatcttc acaaggtgga aggccaggat gagtgtaacc
     2401 acgaaaccga aagggctgaa aacccgctgc agcttcgcca ggattccgga ggcaacgcag
     2461 gttccggatc tgatcagggt ccagaaggag tcttacgagc gcttcctgca gacggacacg
     2521 ccgaacgaca agcgcggtga tttcggactg cacggcatct tcaagagcat ttttccgata
     2581 acggactaca acaagacgtc ttcccttgaa tacaagggct acgtcctgga tgagccgaag
     2641 tacgatgtcg aggaatgccg ccagcgcggg ataacgttcg cctctccgat gcgcgtgacg
     2701 gttcagcttg tgatgtacga cgttgacccg gacacaggcg tccgcgacgt tcgtgacatc
     2761 a
//
LOCUS       DAOPPA010000445         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_39976_length_2753_cov_3.934396, whole genome shotgun sequence.
ACCESSION   DAOPPA010000445 DAOPPA010000000
VERSION     DAOPPA010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39976_length_2753_cov_3.934396"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..2187
                     /locus_tag="PKH54_11815"
     CDS             253..2187
                     /locus_tag="PKH54_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOD00624.1"
                     /translation="MKAAFVGLAVLMAVSVMAGCGRDRQSGPETGLEALYRLDRDFIS
                     HRGAHTYIEAVGPDSGTSAPNALYSREYSDLMSHFGQYKPVVSPLLELDPAMGCVLDA
                     RTWFPFEMEQVFVCTEGSIAVEFAYVGGQDFAVRVAARQDFAGVSIGVRGCGYDNGDG
                     MLTTELDADAGLVFMKQTITLYSFWNVETHENLSYTIAARADMLDVPLSLENSCWTAS
                     LPADRTSVVTYRVERDWAVGTAPDVPSASPQEPLDSTAFSVANLESIEELEAWFKDSP
                     VKDIGDPLSMMSWYLFWENTADPWGSRWPNEAVVPSKRHYFRGVWLWDAGFIAIALSR
                     GNEQAHALGADQIRNIVASQAPDGRLPREVWAHDAGTGFQPPGILTWAVWELARWDQD
                     VELVEEFYGALVAGHQWFVNNTDSDGDGRCEWTREDSGMDTSPRFDDGPVEGVDLQSW
                     LALDAQLLAGMADRLGRTGEAEAWRAEAEDRKADIRENFWDPADGMFYDLKIDDSAAD
                     KFVRSPTPVAFIPLLVGAATQEQALAVASRLNDAEFFDAPFPLPTTPLTGPAYDSDNY
                     WRGPVWIVMNALAWFGLMNYDLHEQANQLRTETLSMMRLSPTTWEYYDSTSGQGIGSP
                     DFMWSAAFYLMMTYLITPPF"
     gene            2273..>2753
                     /locus_tag="PKH54_11820"
     CDS             2273..>2753
                     /locus_tag="PKH54_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00625.1"
                     /translation="MNMKLSPAVMAVLFLLPIAGAAGCGDSGNGSADLPRDASARDSA
                     GDIQSDVAADAADASGGDGVERDNAGPTDTFADALDDAEASPVDSGTDDFEDPGLGDL
                     ADDGVELDADADSALDSFDSQTVDAGDVAPIQDAVTLGCAPTGPAAPVVDCAHPVEYQ
                     "
BASE COUNT          493 a          778 c          906 g          576 t
ORIGIN      
        1 gactgagcta caggcgcgtc aaaagcggac tgatggtaca tggaacaagg tgaggtcgtc
       61 aagtgcggtt gacgcggctc agcgttggta aagggcaggg agggccacgg aaggaatccc
      121 gggttcctcg acgacgacag ggcgcacgcc ccgcgattcc cgaggtgcgg cagtgctgaa
      181 aaatttgaca ccgtttcccg gtaaatcatg atagcgcccg gtcttgactt tggctggggg
      241 ccagattccg gaatgaaggc ggcatttgtt ggactcgcgg ttcttatggc agtgtcggtg
      301 atggcaggct gcggccgtga tcggcagtcg gggccggaga ccggcctgga ggcgctttac
      361 cgcctggatc gcgacttcat cagtcaccgt ggcgcacaca cctacatcga ggctgtcggg
      421 cccgattcgg gcaccagtgc gccgaacgcg ttgtattccc gcgaatacag tgacctgatg
      481 tcacatttcg gtcagtacaa gccggtcgtc agtccgctgc tggagttgga tccggcaatg
      541 ggttgcgtcc tggatgcgcg cacatggttc ccgttcgaga tggagcaggt ttttgtctgc
      601 acggaaggca gcatcgcggt cgaattcgcc tatgtcggcg gccaggactt cgcggttcgc
      661 gtcgctgcca ggcaggactt tgccggcgtt tcgatcggcg tgcgcgggtg cggttatgac
      721 aacggggatg ggatgctgac gaccgaattg gacgccgatg ccggtctcgt attcatgaag
      781 cagacaatca cgctttactc tttctggaac gtcgagaccc atgaaaacct gtcctatacg
      841 attgcggccc gggccgacat gcttgatgtg cctctttctc ttgaaaactc ctgctggacg
      901 gcatcgcttc cggccgacag gaccagcgtc gtgacgtatc gggtcgaacg ggactgggct
      961 gtcgggaccg cgccggatgt gccgtccgca tccccgcagg aacctttgga cagtaccgcg
     1021 ttcagcgttg ccaacctgga aagtattgag gaacttgagg catggttcaa ggattccccg
     1081 gtcaaggata tcggggatcc gctttccatg atgtcgtggt acctgttctg ggaaaacacg
     1141 gcggacccct ggggaagccg ctggccgaac gaggccgtcg ttcccagcaa acgccactac
     1201 ttccgtggcg tctggttgtg ggacgcgggg ttcatcgcaa tcgcgctgtc gcgaggaaac
     1261 gagcaagcgc acgcgttggg tgcggatcag attcgcaaca tcgtcgccag ccaggctccg
     1321 gatggacggc ttccccgcga ggtctgggcc catgacgccg gcaccggttt ccagccgccc
     1381 gggattctga cctgggctgt ctgggaactg gcgcgttggg accaggacgt ggaactggtt
     1441 gaagaattct atggcgcgct ggtcgccggc catcagtggt tcgtcaacaa taccgacagt
     1501 gatggcgacg ggcgttgcga gtggaccagg gaagacagcg gaatggatac gtcgccaagg
     1561 ttcgacgacg ggcccgtcga aggcgtcgat cttcagtcct ggctggcgct ggatgcgcaa
     1621 cttcttgccg gcatggcgga caggcttggc cggacgggcg aggcggaagc ctggcgggcc
     1681 gaggctgaag accgcaaggc cgacatccgt gaaaacttct gggatccggc cgacgggatg
     1741 ttttatgacc tgaagatcga tgattctgcc gccgacaagt ttgtcaggag tccgaccccg
     1801 gtcgcgttca ttccattgct tgtcggagcg gccacgcagg aacaggccct ggcggttgcc
     1861 tcaaggttga acgatgccga atttttcgac gcgccgttcc cgcttccgac cacgccgctc
     1921 acgggcccgg cgtacgattc ggacaattac tggcgcgggc cggtatggat cgtgatgaac
     1981 gcgctggcct ggttcgggtt gatgaattac gacctgcatg aacaggcgaa ccagcttcgt
     2041 acggaaacgc tttcgatgat gcgtctgtcg ccgaccacgt gggagtatta cgactcgacg
     2101 agcgggcagg gtatcggttc gccggatttc atgtggtctg ccgcctttta cctgatgatg
     2161 acgtacctga ttaccccgcc gttctgaccg tttcccatcc ggagtccttg tccgtccggt
     2221 cgattccgtg cattattgac cgcaagggtc gttacgttcg cataaacagg taatgaatat
     2281 gaaactgtcg cccgccgtga tggcggttct tttcctcctg ccgatcgcgg gggcggcggg
     2341 ttgtggcgat tcgggcaatg gttccgccga tttgccgcgc gacgcgtccg cccgcgattc
     2401 agccggcgac atccagtcgg acgtagccgc cgacgcggcc gatgcttccg gcggggacgg
     2461 cgttgaacgt gataatgcag gtccaaccga cacatttgcc gatgcacttg atgatgcgga
     2521 agcctccccc gtggattccg gtactgatga ttttgaggat cccggcctgg gtgacctggc
     2581 cgacgatggc gtggaactgg acgccgatgc tgattcggct ttggattcat ttgattccca
     2641 gacagtcgat gccggggatg tcgccccgat tcaggatgcc gtgacccttg gctgcgctcc
     2701 gacgggcccg gccgcacccg tcgtcgattg cgcgcatccc gtcgagtatc agg
//
LOCUS       DAOPPA010000446         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40029_length_2751_cov_3.245920, whole genome shotgun sequence.
ACCESSION   DAOPPA010000446 DAOPPA010000000
VERSION     DAOPPA010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40029_length_2751_cov_3.245920"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKH54_11825"
     CDS             complement(<1..495)
                     /locus_tag="PKH54_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00626.1"
                     /translation="MKKIRNALVKALDEIGIAHQDGPEDSLVFSLKNVEKDDSIPFFV
                     SFLSDDDEFVTMGIHVWECSQSMFTFGMAIAGNVNATSKVAKVFPLHMPVSDGDCECD
                     DEGCDCDMGSDVCDCGCDQDDCGDEACDCDDDCGCGCREPAVRAHFFVTSQFFWTGET
                     IPTDV"
     gene            complement(499..1647)
                     /locus_tag="PKH54_11830"
     CDS             complement(499..1647)
                     /locus_tag="PKH54_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00627.1"
                     /translation="MASTDRFPGLRFAKTLAAILSLISISCASTGGNHPDDTDAFGIT
                     DTVVAGDTGDSTETTDATSTTETADAGLVVDAGYQFDTPDMGEISDAPDTGDTVDTPD
                     AGDTADAPPPTRSCNGMPELCDRPLDQIAFVTTHNAMSNQDDSFLVPNQYSSVTRQLQ
                     AGVRAFMLDTWYDNTQDPPPSDDVYLCHGVCVFGMRLLSGTLADIRAFLLDNPDDFVV
                     IIFESYVSAAHTLASFDQAGVTEMLLNPLPGQPLPTLAQAIDGGKRMLVLTDSGGGGF
                     PGYLSVWDWAFETHWSNKVPDDLSCATNRGSDENPFFILNHFLTDPTALPELAAQINE
                     NPFFMDRAMACWKERNHIPNFVTIDFFEIGDVLDVVSQLNLAVAPIRR"
     gene            1833..2666
                     /locus_tag="PKH54_11835"
     CDS             1833..2666
                     /locus_tag="PKH54_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD00628.1"
                     /translation="MEHLFQYLLFIPIGFIAGGLGTFVGAGGGFLVVPFLAFWDPQLA
                     PQTITAISLGVIFFNSASGSIRYARTRRIDYVSGVTFAVATIPGAALGAAISGLIPRQ
                     AFNIMFGVFMLAVAVFLMLKPGRAVSGSKPVSGQFRRIMTDRSGAVYDFSYNRSIGVA
                     SSGIVGILSSLLGIGGGIIHVPVMTRLLNFPVHLATATSHFILVITALTGATVHLVYG
                     RYFQPEAALMLVALTIGVIPGAQLGARLSEKAKPTWILRGLSFVILLVALRLLIGFGA
                     S"
BASE COUNT          547 a          779 c          806 g          619 t
ORIGIN      
        1 cacgtccgtc ggaatggttt cacctgtcca gaagaactgc gatgtcacga agaagtgggc
       61 cctgaccgcc ggttcgcggc agccgcaccc gcagtcgtca tcgcagtcgc aagcctcgtc
      121 cccgcaatca tcctggtcgc acccgcagtc gcaaacgtcg gaacccatat cgcaatcgca
      181 gccttcgtcg tcgcactcgc agtcgccgtc cgaaaccggc atgtgaaggg gaaaaacctt
      241 cgcaaccttt gatgtggcat tcacgttgcc cgcgatcgcc attccaaaag tgaacatgga
      301 ctggctgcat tcccagacat ggatgcccat cgtgacgaat tcgtcgtcat ccgaaagaaa
      361 ggacacgaaa aacggtatgc tgtcgtcctt ttcaacgttc ttcagcgaga aaacaaggct
      421 gtcttccgga ccgtcctggt gggcgattcc aatttcatca agagctttca caagggcgtt
      481 tcgaatcttt ttcacaggtc acctccggat tggcgccacg gccaggttaa gttgcgaaac
      541 gacgtcaagc acgtcgccga tttcgaagaa gtcgatggta acgaagtttg gaatgtggtt
      601 gcgttccttc cagcaagcca tcgcgcgatc catgaagaac gggttttcgt taatctgggc
      661 ggccagttcc ggcagtgcgg tcggatccgt caggaaatgg ttcaggatga agaacgggtt
      721 ttcgtcgctt ccgcgattgg tcgcacagga aaggtcatcc ggaaccttat ttgaccagtg
      781 tgtttcgaag gcccaatccc agacggaaag atatcccgga aaaccgccgc cgccgctgtc
      841 ggtaagaacc agcatccgtt ttccgccatc gattgcctgt gcgagggttg gaaggggctg
      901 gcccggcaag ggattcaaga gcatttcagt gacgccggcc tgatcaaaag acgcaagggt
      961 gtgcgcggcc gaaacatagc tttcgaaaat gatgacgacg aaatcatcgg gattgtccag
     1021 aaggaaggcc ctgatatccg caagcgtccc ggacagaagc cgcatcccga aaacacagac
     1081 gccgtgacaa agatagacgt cgtcggacgg cggcggatcc tgggtgttgt cgtaccaggt
     1141 gtccagcatg aaggcgcgaa ccccggcctg caactgcctc gtgacggatg aatactggtt
     1201 gggtactaag aacgagtcgt cctggttcga catcgcgtta tgagtggtga cgaaggcaat
     1261 ctgatccaga ggccggtcgc acagttcggg catcccgttg cacgagcgtg tgggcggtgg
     1321 agcatccgcc gtgtcgccgg cgtcgggggt atcgaccgtg tcgccggtgt ccggcgcatc
     1381 gctgatttcg cccatgtcgg gcgtgtcgaa ctgatagccg gcatcaacca caagcccggc
     1441 gtcagccgtt tcggtcgtgg atgtggcgtc ggtcgtttcc gtggaatcac ccgtatcgcc
     1501 ggcgaccacc gtatcggtaa tcccaaaggc gtcggtgtca tccggatggt tgccgcccgt
     1561 gctggcgcag gaaatcgaaa tcaaggacag gatggccgca agcgtcttcg cgaaacgcaa
     1621 accagggaag cggtctgtcg aagccatcct cacacccggc aaactggacg attttgcgaa
     1681 atccgtccct gaaagggaaa ttcgccttgc gtatggtatc ccggtttgtg ccacgcgcgg
     1741 tcaatcaatt ttttgtaact ggccgaacat gtgcgcttgt actagactgg cgcaggcatc
     1801 gacgggtcgc aaccgggccg ggattgtttt gcatggaaca cctttttcaa taccttcttt
     1861 tcattccgat tggtttcatc gcgggtggac ttgggacttt tgtcggtgcg ggaggcggct
     1921 tccttgtggt gccattcctg gcgttctggg atcctcaact tgccccacag acaatcacgg
     1981 ccatctcact gggtgtcatt ttcttcaatt ccgcctccgg cagcatccgt tacgcgcgga
     2041 caaggcgcat cgactacgtg tcgggagtca ctttcgccgt tgccacaatc cccggagctg
     2101 cccttggagc cgccatttcc ggcctgattc cccgtcaggc cttcaatatc atgttcgggg
     2161 ttttcatgct ggccgtcgcc gtcttcctga tgttgaagcc gggcagggcg gtaagcgggt
     2221 cgaagcccgt gtcggggcag ttccgcagga taatgaccga cagatccggt gccgtttacg
     2281 acttcagtta taaccgatcg atcggcgtgg ccagcagcgg tatcgtcggg attctttcca
     2341 gcctgcttgg gatcggtggc gggatcattc atgtgcccgt gatgaccaga ctgctgaatt
     2401 ttccggttca cctggcgacc gccacgtcac atttcatact tgtgattacc gctttgacgg
     2461 gtgccacggt tcatctggtt tacgggcgct attttcaacc cgaagcggcc ttgatgctgg
     2521 tcgccttgac gattggcgtg attccgggag cccaactggg cgcgcgcctt tcggaaaagg
     2581 ccaagccaac ctggattctt cgcgggcttt cgttcgtcat cctgcttgtg gccctgcggc
     2641 tgttgattgg attcggcgct tcctgaaatg ggcccctgtc cattgtcttg aacctcccga
     2701 tgtgatagaa atcctgcgcc acgcagcggt ggcataggcg cggcggtcga c
//
LOCUS       DAOPPA010000447         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40130_length_2745_cov_3.781413, whole genome shotgun sequence.
ACCESSION   DAOPPA010000447 DAOPPA010000000
VERSION     DAOPPA010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40130_length_2745_cov_3.781413"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..612
                     /locus_tag="PKH54_11840"
     CDS             142..612
                     /locus_tag="PKH54_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00629.1"
                     /translation="MNGADSKSVVDVLVKALGDECVVVKNHLDNPDLLRQKRWVVEDL
                     GGALHILYQGSTLGLIDLGELSFATDFGCNAENLKDIPQSIADQAREWAEQEMWPAWE
                     ARGYIVDPGAHPENGWDPLERLWLLNVIKKVEFVEALVEEIRWAANQERIQFIQ"
     gene            complement(752..>2745)
                     /locus_tag="PKH54_11845"
     CDS             complement(752..>2745)
                     /locus_tag="PKH54_11845"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD00630.1"
                     /translation="QMGLFGDAQVMHDEQERARAIKSRRRVTVVIGNPPYRRVDKKGS
                     GRGGGGWVLDGSVPGRQDLDQSLFDDILDVAKQHTIFSHHASLYNLYVYFWRWAIWKA
                     FEAHGPGPGVLAFITASSWLTGPGFVGLRKLVREVADEAWILDLGGDNKGAVTEKNVF
                     AIQTPVAITILARFGEGNKTHPAQVHYRRIQGDRTSKLEVLKAIGESDRPLEGTWQDV
                     PSGWLDRMMPVTGTTEWDAMPALADLIPWQQPGCKFNRLWPIAPDPKSLDERWKSFVL
                     APVEKDRVTQLTRGRNPGPSRETLFQTTATGRNIETSVSGLSRLADLRADAPSQPVVR
                     YGYRSFDRQWAFDDPRMAKTDSPALWQSVSDRQLFLVSRTTEAIGPGPAMVTTDAVPD
                     LHHFQGAEGGKDVLPLYRDRACLQPNVTAGLLPALGRLLELSHVPCPEDLAAYIYALL
                     STPAYQQRFASELETPGPRVPITRDSGLWNRAVVIGRRLLWLHTYAERFRDPNEARGT
                     RVPKVEGLGWLAPVRRMPGTPQDVSYDPSLQQIMIGDGVVSGVKPEVWAFTVSGMQVV
                     PKWLGYRTRSGAGKVVSSANPLDRIRPSEWLDQWNDELLDLLRVLTLTVEGFPEQQTL
                     LEDICAGPLIAADELPAPDAAEREPPPTEKRFDPGALFG"
BASE COUNT          566 a          853 c          791 g          535 t
ORIGIN      
        1 tcaagaaggt aaactccgtc gccgaactca tcgcagaagc ccgcttcctg tccagaagca
       61 acctggacga cacgttcgga atggggtaag tcgttttgaa cggtttggat taatgacagg
      121 ggccaaccca cgggaggttt tatgaacggc gcagattcca agtcagtcgt tgatgttctg
      181 gtgaaggccc tgggcgacga atgtgttgtc gtcaagaacc acctcgacaa ccccgatctt
      241 cttcggcaga aacggtgggt tgtggaagac ctgggcgggg cgctccacat tctttaccag
      301 ggttccactc ttgggctcat cgacctgggg gaactgtcat tcgccaccga cttcggttgc
      361 aacgctgaaa atctcaagga tattccacaa tccatcgctg accaagccag ggaatgggcc
      421 gaacaggaaa tgtggccggc atgggaagcg cgtggttaca tcgttgaccc cggtgcccac
      481 ccggaaaatg gatgggatcc cctcgaacgc ttgtggctgc tcaacgttat aaaaaaggtg
      541 gaatttgttg aagccctcgt tgaagaaatc cgctgggccg ccaatcagga aagaatccag
      601 ttcattcagt aggttttggg gcccacagtg ccaattccat tcttcagaac aattaaaaca
      661 accttccgac ttgattacat catcccaacg acggacatgt ttttacgatt cccgcctcga
      721 agaaacgcgc tgactgttcg cctgcctcgc cctacccgaa cagcgcgccc gggtcgaacc
      781 gcttctctgt cggtggcggt tcccgctcag ccgcgtcggg cgctggtagt tcgtccgccg
      841 cgatcaatgg acctgcgcag atatcctcca gcaaggtctg ctgttccggg aatccctcca
      901 cggtcagcgt caacacgcgc agaagatcca ggagttcgtc gttccactga tccagccact
      961 cgctgggcct gatccgatcc agggggttcg ccgatgacac tactttcccc gccccggatc
     1021 gcgtgcgata tccgagccat ttcggcacga cctgcattcc ggagaccgtg aaggcccaga
     1081 cctcgggctt tacccctgag acaacgccat ccccgatcat gatctgttgt aaggacggat
     1141 catacgaaac gtcctgcggc gtccctggca tgcgccgtac cggcgccagc caaccaagtc
     1201 cctcgacttt cggaacacgc gtgccccggg cctcgttcgg atccctgaac cgctcggcat
     1261 aagtatgcag ccacaacagg cgccgcccga tgacgaccgc ccggttccac aggccggaat
     1321 ccctcgttat gggaacccga gggcctggtg tttccagttc ggaagcgaag cgctgttgat
     1381 aagcgggggt cgaaagcaac gcgtatatat atgcggcaag atcttcagga cacgggacat
     1441 gcgacagttc cagcaggcgg cccagggcag gcagcagtcc tgccgtcaca ttgggttgca
     1501 ggcaggcccg atcgcggtac aacggcaaga cgtccttgcc gccctccgca ccctgaaagt
     1561 ggtgcaagtc cggcacggca tcggtcgtaa ccatcgccgg accagggcca atcgcctcgg
     1621 tggtacgact gaccaggaac aactgccgat ccgacactga ctgccagagt gccggcgaat
     1681 ccgtcttggc cattcgggga tcatcaaacg cccactggcg gtcgaagctc cggtatccgt
     1741 aacgcacgac gggctgcgag ggggcgtcgg cgcgaaggtc ggccagacgg gacagcccgg
     1801 agaccgacgt ctcaatattc cgcccggtgg cggtggtttg gaacagagtc tcccgagagg
     1861 gccccggatt ccggccccgg gtcagttggg taacgcggtc cttctcgacc ggtgccaaaa
     1921 caaaggactt ccatcgctca tcgagggact tgggatccgg ggcaatgggc cacagccggt
     1981 tgaacttgca tccgggttgc tgccaaggga tcaggtccgc cagggccggc atcgcgtccc
     2041 attccgtcgt gccggttacc ggcatcatcc ggtccagcca gcccgacggc acatcctgcc
     2101 aggttccttc caacggtcga tcggattcgc cgatcgcctt cagaacctcc agcttcgaag
     2161 tccgatcgcc ttggatccgt cgataatgaa cttgggcggg gtgcgttttg ttgccctccc
     2221 cgaatcgtgc cagaatcgta attgcaaccg gggtttggat ggcgaagacg ttcttctcgg
     2281 taacggcacc cttgttgtcg ccgcccaagt ccaggatcca tgcctcgtcc gccacctcgc
     2341 ggaccagttt ccgcaaaccg acgaagccag ggccggtcag ccaactgctg gcagtgatga
     2401 aggctagaac acctggcccg gggccatggg cctcgaaggc cttccatatc gcccagcgcc
     2461 agaagtaaac ataaaggttg tacaggctgg cgtggtggct gaaaatcgtg tgctgtttag
     2521 caacgtccaa aatgtcgtca aacaggctct gatccaaatc ctggcgtcct ggcaccgatc
     2581 cgtccaagac ccagccgcca ccgccgcgac cgctgccttt cttgtccacg cgacggtaag
     2641 gaggatttcc gataactacg gtgacgcggc ggcgcgactt gatggcgcgc gccctttcct
     2701 gttcgtcgtg catgacctgc gcatcgccga aaaggcccat ctggg
//
LOCUS       DAOPPA010000448         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40223_length_2740_cov_3.726257, whole genome shotgun sequence.
ACCESSION   DAOPPA010000448 DAOPPA010000000
VERSION     DAOPPA010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40223_length_2740_cov_3.726257"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKH54_11850"
     CDS             complement(<1..798)
                     /locus_tag="PKH54_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00631.1"
                     /translation="MKLFCINCGEGFAIDDSSVGLDGATVKCVHCAVEQPVFTRRKSL
                     PPGSPGMTVSGNLINPLSEVPSPPPAPPKAVVDASPDASTGSGFRKPLSSMWAGKSGS
                     GTGQVEDIQGDPTVKMVPPPVDAGEDLSMPPPVDDGSFVIGGVDDSVPEISIDVSGGP
                     FTVASPSLLSLEFPDFSVLDEWSRGIEKLEKYTVSDRTGNRSQLGDFLRKRKQGGRAA
                     AVKAAMRTTGETGNDTITDILVGGKGVGNRPSSITQQFEFKVVEEKKR"
     gene            1026..>2740
                     /locus_tag="PKH54_11855"
     CDS             1026..>2740
                     /locus_tag="PKH54_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOD00632.1"
                     /translation="MSGNKKLTKPETGAAVQKRRVREAEVHGLVIALFGVILLLSLVS
                     FDRRDLQLEAGNNQIDNFMGLFGARLADIFLSGFGVIAFVLALGGLLIGLKAVLRRPF
                     QAGVLRIIAPAAGLLFLAVTIHVLAVDHIIFDHYQPGGVIGASVGELLLNLFNRSGTL
                     LISITALSLATLYLAGITIRSALRFTGRQIRTGAGKAVSGLREQASRRRRKAAEMREK
                     GDVEPEVSVVKAIDLTPIQTSVEDSIPELDQTVIDRPRPPQGSPTANPADAMPEEPAP
                     EPAWTASGRDATPIPDVPVTATGRRHGTGIAAATPDRPGENVPKRIDEGQVTKFDIVA
                     PAEMDARQSPIPIVIHRTQSSPAVEHPDTVPATGGDHRTRAQVTSQRATGGASGSTAT
                     HAVPPTASGDWKTEVSAAVSPATSQPASAMTVDGSDGGNRADKAPDASNGTTVVASPA
                     ADQEAPAIIVNRRAKARVQEDLLLPPPPRGPWEPPSLSLLDFVAPAQREIDQDFLRSN
                     AEKLEAKLLDYKVQGKVVGIHPGPVVTMYEFLPAAGVRISQIANLADDLTMALKATSI
                     RIVAP"
BASE COUNT          566 a          891 c          796 g          487 t
ORIGIN      
        1 ccgcttcttt tcctcgacaa ccttgaattc gaactgctgg gtgatgcttg acggcctgtt
       61 cccgacgccc tttccaccga ccaggatgtc ggtaatggtg tcgttgcccg tttctccggt
      121 cgtccgcatg gccgccttga ccgccgcggc gcgaccgccc tgcttccttt tccgaaggaa
      181 gtcgcccagt tgcgaacggt taccagtccg atccgaaaca gtatattttt ccagtttctc
      241 gatgcctcgt gaccattcgt cgagcacgct gaaatcggga aactcaagcg acagcaatga
      301 tggggaagcg accgtgaacg gcccgccgga gacatcaatc gaaatctcgg gaaccgaatc
      361 atccacgccg ccaatcacga acgagccgtc atcgaccggg ggcggcatcg acaggtcttc
      421 gccggcatca acaggcgggg gaaccatctt gacggtcgga tcaccctgga tatcctcgac
      481 ctgccccgtg ccggaacccg acttgccggc ccacatcgag ctcagcggct tacggaaccc
      541 ggaacctgtg gatgcgtccg gcgaagcatc gacaacggcc ttcggtgggg cgggcggggg
      601 ggacggaact tccgacaggg ggttaatcag attcccggaa acggtcattc ccggggaacc
      661 gggcggaagg cttttccgcc ttgtgaatac aggctgttca accgcgcaat ggacacattt
      721 gaccgtggcg ccgtcaagcc ccaccgacga atcgtcgatc gcgaagccct caccgcaatt
      781 tatgcaaaac agcttcatgc aagcattctg ccaccgggaa ataccggctt caaccggccg
      841 caccgaaaga cgtccggaag aaaatcgcga ttagtatata ccatcaccat cgccccgcca
      901 cgtcttcaaa ggcattttcg gacaaaaatt cggactttga cacaacatta atatcgtgcg
      961 ccgttttggc acgcctgctg acgcggtgat agcctaaggg cggacttctt catggttcac
     1021 atccaatgtc cggaaacaag aagctgacaa aaccggaaac gggtgcggca gtacagaagc
     1081 ggcgtgttcg cgaggccgag gtgcacgggc tggtcatcgc gcttttcggc gtaatccttc
     1141 tgctctccct ggtttcattc gatcggcggg atcttcaact ggaagccggc aacaaccaga
     1201 ttgacaactt catgggcctt ttcggggccc ggctggccga catcttcctt tccggattcg
     1261 gggttatcgc attcgtcctg gctctgggag gtcttctgat cgggctgaag gccgtccttc
     1321 gacggccgtt tcaggccgga gtgctgcgca ttatcgcacc ggcggcgggg ctgcttttcc
     1381 tggcggtcac gattcacgtc ctggccgttg accacatcat cttcgaccat tatcagcccg
     1441 gcggcgtcat cggcgcatcg gtcggggaac ttctgcttaa ccttttcaat cgcagcggaa
     1501 ccctgctgat atcgattacc gccctgtcgc ttgcgacgct ttaccttgcg ggcatcacca
     1561 tccggtcggc ccttcgcttc accgggaggc agatcagaac cggcgccggc aaagccgttt
     1621 caggcctgcg cgaacaggct tcaagacgca ggcggaaggc ggccgaaatg cgggaaaaag
     1681 gcgatgtgga acccgaagta tcggtcgtaa aggcaatcga cctgacacca attcagacat
     1741 cggtcgagga ctcgatcccc gagcttgatc agaccgtaat cgaccgaccg cgcccgccgc
     1801 aggggtcgcc gacagcgaat ccagccgatg cgatgcctga agagcctgcg ccggagcctg
     1861 cctggaccgc gtccggacgc gacgccaccc cgatccccga cgtgcccgtt accgcaaccg
     1921 gcagacgaca cggaaccggc atcgcagcgg ccaccccgga tcggccaggg gagaatgtcc
     1981 cgaagcggat cgacgagggc caggtcacga aattcgacat tgtcgccccc gcggaaatgg
     2041 acgccagaca gtcaccgatt ccgatcgtca ttcacaggac ccagtcctcg ccggcggtcg
     2101 agcatcccga caccgtacct gcgaccggtg gcgaccacag gacccgtgcc caagtcacat
     2161 cgcaacgcgc gaccggcgga gcgtccggct cgacggccac gcacgcggtg ccgccgaccg
     2221 cttccgggga ttggaagacg gaggtcagtg ccgccgtctc ccctgcgacc agccagccag
     2281 catcggcgat gactgtcgat ggcagcgacg gcggaaaccg ggcagacaag gcccccgacg
     2341 cttcgaatgg cacgaccgtc gtggcttcac ccgcagcgga tcaggaagcg cccgccatca
     2401 tcgtcaaccg gagagccaag gcccgggttc aggaagacct gcttcttcca cccccgcccc
     2461 gcggaccgtg ggaaccaccg tcgctttccc ttctggattt cgtcgccccg gcccagcgcg
     2521 aaatcgacca ggacttcctg cgctcgaacg ccgaaaagct ggaagccaaa ctgctggact
     2581 acaaggttca gggcaaagtc gtcggcatcc atccggggcc ggtcgtcacc atgtacgaat
     2641 tcctgccggc agccggagtc aggatcagcc agatcgcgaa cctggctgac gacctgacga
     2701 tggcactgaa ggcgaccagc ataagaatag tggcgccgat
//
LOCUS       DAOPPA010000449         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40322_length_2735_cov_3.309328, whole genome shotgun sequence.
ACCESSION   DAOPPA010000449 DAOPPA010000000
VERSION     DAOPPA010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40322_length_2735_cov_3.309328"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1575
                     /locus_tag="PKH54_11860"
     CDS             265..1575
                     /locus_tag="PKH54_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00633.1"
                     /translation="MSASSSRFGILAAVAVVSLASIQASGNPLDMFGAGAANIAMGGT
                     GTATADSYEAAYYNPAALGLAERVEFGAGASVYRPWLEARFNSYDPVGKVTSPVSGRR
                     FDRAQAFLEAGVVAPIPLGKDLGRHLFFGLHVYSPADVVYSVVAREATEPAFPLYEDR
                     NRRLVLNAALSGRYKWLMFGLGISVLPTVRGTVRMDLADTSLQDYLGIDVGFAVSPNA
                     GLLFEPIDGLTLGIAYRAAGKTRIELPVDAAVTEVPVFLTVNAATFWTPHQVSFGIGW
                     KGKNYRVAGDVVFSMFSGFQMASPNLNLFKDSGRDQVSSSGTVVDAGFRDTASVRLGG
                     EFRPIKPVALRAGFSWTQSPLSAQSGITNMLGGDQFGGSFGIGFDAAEVGGPHIKVDA
                     YFMAAAVIDNTDSKLELMPSNPGYPSIGGGGWFLNSGLSIRFGF"
     gene            1584..>2735
                     /locus_tag="PKH54_11865"
     CDS             1584..>2735
                     /locus_tag="PKH54_11865"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00634.1"
                     /translation="MSVKICLLAVLATVSCGRGPGVDTDGDGLSDLQELLFGSDPAVA
                     DTDHDGVPDGRDDNLFSPDPAIRLTASPAFQDEQQGRCVVVGIRAIDGRGFPVAGARV
                     DLSWGLGDLQDVVTDESGFGNVTVCWPQGVDTKFMAKSRDLGGRHPEIQAELELSLHH
                     LVVPGVNTSPWEGAGKIDGRLKVVALYRNRVGLLKPFQGASVHVGAEGVQIPFMTTGP
                     EGVVDFVVPGLKGPVDVTVGAPGHRYVTYFRTEGSVISVILDPLDVISEDAVTGFGTI
                     SGKVKGFFGEGGGPKFPPGILITDPRTPVAIVQLAINGRPLSSMSMGGVLEEPSGTGI
                     WEAIPSNMAVCQISSPDQKDCGEEGVFSLRNIPEGQHLVFALGGTVGDVV"
BASE COUNT          474 a          791 c          855 g          615 t
ORIGIN      
        1 ggttgacgga ccgtcgtcac aaccggctga aatcacgagc atggaaccaa gaatcagtgg
       61 aagcaggttg cgcgacgtcc tcatgggcgt aatccccgga aaaatcaggc aggacattat
      121 aatcgtttga agaatggaat tgccgttaaa aatcgatggg caacggttcg ggtgaaacgg
      181 gtataatacc gccgcccgaa ctgcgggtaa tccggcccga tcgcaggccg gtattggatt
      241 tttcatatcg cgggaccggt attgatgtct gcgtcgtctt ccaggttcgg gattctggcg
      301 gcagtcgctg tcgtttccct ggcttcaatt caggcttccg ggaatcccct ggacatgttc
      361 ggcgccggcg ccgccaacat cgcgatgggc ggaacgggaa ccgcaacggc cgattcctac
      421 gaggcggctt actacaatcc ggccgcgctc ggccttgccg agcgagtcga attcggtgcc
      481 ggggcttccg tatatcgacc atggcttgag gcgcgcttca attcttacga tccggtcggc
      541 aaggttacgt cacccgtttc cggacgcagg ttcgaccgcg cccaggcgtt cctggaggcc
      601 ggggtggttg ccccgatacc gcttggaaag gatctgggcc gccatctttt cttcggtctt
      661 cacgtgtatt ccccggccga cgtggtctat tccgtcgttg cccgcgaggc cacggagccg
      721 gcgttcccgc tttacgagga tcgcaaccgt cgcctggtcc ttaatgccgc tttgtccgga
      781 cgatacaaat ggctgatgtt cgggttgggg atttccgtcc tgcccacggt caggggaacg
      841 gtcaggatgg acctggccga cacttcgctt caggactacc ttggaatcga cgtcgggttc
      901 gcggtttcgc cgaatgccgg tctgctgttc gagccgattg acggattgac cctgggcatc
      961 gcctatcgcg ccgccggaaa gaccaggatt gaacttccgg tcgatgcggc agtaaccgag
     1021 gttcccgtgt tcctgactgt caacgccgcg actttctgga caccccacca ggtttcgttc
     1081 ggaatcggct ggaagggaaa aaattaccgc gtcgccggtg atgtcgtctt ctccatgttt
     1141 tccggcttcc agatggcgtc gccaaacctg aaccttttta aggattccgg acgtgaccag
     1201 gtttcatcca gcggaaccgt ggtcgatgcc ggattccggg acactgcttc cgtccgtctc
     1261 ggcggcgaat tcaggccgat caagccggtt gcccttcggg ccggtttttc gtggacccag
     1321 tcgccgttgt cggcgcagtc cgggatcacg aacatgcttg gcggcgatca gttcggcggg
     1381 tcgttcggca tcgggttcga cgccgccgag gtcggtggtc cgcacatcaa ggtcgatgcc
     1441 tatttcatgg cggcggccgt catcgacaat accgattcga agctggaatt gatgccgtcg
     1501 aatccgggtt atccttccat cggcggcggt ggttggttcc ttaactcggg tctttcgata
     1561 aggtttggtt tctgatgcga ttgttgtcgg tcaaaatctg tctgctggcc gtccttgcga
     1621 ccgtttcgtg cggtcgcgga cctggcgtcg ataccgatgg cgacggcctt tccgatcttc
     1681 aggaactgct tttcggatcc gacccggccg tcgccgatac cgatcacgac ggcgttccgg
     1741 acggccgcga cgataatctg ttttccccgg atcccgcaat caggctgacc gcaagtccgg
     1801 cattccagga cgagcagcag ggccgctgtg tcgtcgtggg gatccgcgcc attgatggtc
     1861 gcggctttcc cgtcgccggg gcccgggttg acctttcctg gggacttggc gatcttcagg
     1921 acgtcgtcac cgatgaatcc ggttttggga atgtaaccgt gtgctggccc cagggcgttg
     1981 acacgaaatt catggcgaaa agccgcgacc tgggcggacg tcaccccgag atccaggctg
     2041 aactggaact ttccctgcac cacctggtcg ttcccggggt caatacatcc ccctgggaag
     2101 gtgccgggaa gattgatggc aggctgaagg tggtcgccct ttacaggaac cgcgtcggcc
     2161 tgttgaaacc gtttcagggg gcgtcggtcc atgtcggcgc ggaaggcgtc cagattccgt
     2221 tcatgacaac cggtcccgaa ggcgtggtcg atttcgtcgt tcccggtttg aagggcccgg
     2281 tcgatgtcac cgtcggggcg ccgggacaca ggtatgtgac ctatttcaga accgaaggat
     2341 ccgtcatctc ggtgatcctt gatccgcttg atgtcataag cgaggacgcg gtcaccggct
     2401 tcggcacgat ttccggaaag gtgaagggct tcttcgggga aggcggcggc ccgaagtttc
     2461 cacccggaat cctgattacc gatccacgta ccccggttgc gatcgtgcaa ctggccatca
     2521 acggacgccc cctttcgtcg atgtcgatgg gaggcgtgct cgaggagcct tccggcacag
     2581 gcatctggga agccattccg tccaacatgg ccgtgtgcca gatttccagt cccgaccaga
     2641 aggattgcgg ggaagagggc gtcttttcct tgcgtaacat cccggaaggg cagcacctgg
     2701 tgtttgccct gggcggaacc gtgggcgatg tggtt
//
LOCUS       DAOPPA010000450         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40495_length_2727_cov_3.444611, whole genome shotgun sequence.
ACCESSION   DAOPPA010000450 DAOPPA010000000
VERSION     DAOPPA010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40495_length_2727_cov_3.444611"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2361
                     /locus_tag="PKH54_11870"
     CDS             178..2361
                     /locus_tag="PKH54_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD00635.1"
                     /translation="MPKVSSKSRISEVQPVEAPPVEVPQVETPPVETPKKPRKPRAAK
                     PVEAPESVETLVAPTVEDDVDVPTFFNMDLSEPLVTALRDLGWETPTPIQSATIPLLL
                     AGRDIVGQAQTGTGKTAAFALPILSRIDVTHQVPQVLVLTPTRELCIQVASVFGQLGS
                     GLRGIRVLPVYGGQGFAEQLSALKKGVHIIVGTPGRVMDHMRRNTLKLDHMHFLVLDE
                     ADEMLNMGFAEDIEWILEQTSKERQTALFSATMPPEIRRIAGKYLRDPEEVTIRNATA
                     TAENTTHRYWITQNYNKFEVLTRILETEEHDGVLIFVKTKSATIEIAEALSECGLPAS
                     ALNGDMKQCDRERTVNRFKDGRQDILVATDVAARGLDVDRISHVINYDPPHDTEGYVH
                     RVGRTGRAGRSGQAIIIATPRERRFLADIERATRQRLEPYEMPSLDGVNRARIEKFKL
                     RLADALKQDGCQPFARIIEEYQEETGVPAISLAAALARQMQGSVPLLIDRMPEPAMDP
                     RPSTNRGPRPAYGSRPPRFGDRAFDRAADRPGDRAAFREGDRAGNREGERPFVREGSR
                     AARGFGDRPAERQFENRQMSGGDRPLRRQAAPGEDLPARRPPVDRAPDPGMDRYRLEC
                     GHTHGVMPGNIVGAIANEAGLRGKDIGHINIFDDYSTVDLPVGMPDRVFSILKKTKVA
                     NRYLDLSLMIQSKLEKIDARRHEDRPPRDRPSRTPDRRTPPARRR"
BASE COUNT          571 a          827 c          846 g          483 t
ORIGIN      
        1 gatcagtggc aggccgcgac ggtccgggcg gacttgaatg ttgattgcag tgaatcaacg
       61 gtccccctga aacgggtttg tgtcagccgc ttaagatccc gagtgtaaaa caggttccgc
      121 ctgcgggaac gaccttgacg gttcgcctgc cggaatcctt gcccacagga atgtcgaatg
      181 ccgaaagtat cgtccaagtc ccggatttcc gaagttcagc cggttgaagc cccgccggtc
      241 gaagtcccgc aggttgaaac gcctccggtc gaaaccccaa agaagccacg aaagccccgc
      301 gccgccaagc cggtcgaagc gcccgagtct gtcgagaccc tggtggcccc gacggttgaa
      361 gacgacgtgg atgtcccaac gttcttcaac atggatttgt ccgaaccatt ggtgacggca
      421 ttgcgcgatc ttggctggga aaccccgacc ccgattcagt ccgcaacgat tccgttgctg
      481 ctggccggca gggacattgt cggccaggcc cagaccggaa cgggcaagac ggccgcgttc
      541 gccctgccga ttctttcaag gattgacgtg acccaccagg ttccgcaggt cctggtcctg
      601 acccccaccc gcgagctttg catccaggtc gcatcggtgt tcggacaact cggatccgga
      661 ttgcgtggca ttcgtgttct gccggtctat ggcggccagg ggtttgccga acagctttcc
      721 gccctgaaga agggtgtcca catcatcgtc ggaactccgg ggcgcgtaat ggatcacatg
      781 cgtcgcaaca cgttgaagct ggatcacatg cactttctgg tgctggacga agccgacgag
      841 atgctgaaca tgggttttgc cgaggatatc gaatggattc tggagcagac gtcgaaggaa
      901 cgccagacgg cgctgttttc ggccacgatg ccacccgaga ttcgaagaat tgccggcaag
      961 tacctgcgcg acccggaaga ggtcaccatc aggaacgcga cggcgacggc cgaaaacacc
     1021 acccacaggt actggataac ccagaactat aacaagttcg aagtgttgac caggattctg
     1081 gaaaccgagg aacatgacgg cgtcctgatt ttcgtcaaga cgaagtcggc caccatcgag
     1141 attgccgaag ccctgtcgga atgcggattg cccgccagtg cgcttaacgg cgacatgaag
     1201 cagtgtgatc gtgaacggac cgtcaaccgc ttcaaggatg gtcgtcagga catcctggtc
     1261 gccaccgacg ttgccgcgcg cggactggac gtggatcgta tcagccatgt aatcaactat
     1321 gacccgccac acgacaccga aggttacgtt caccgggttg gaagaaccgg gcgggcaggg
     1381 cgcagcggcc aggcgataat catcgcgact ccgcgtgaaa ggcgctttct tgccgacatc
     1441 gaacgcgcca cgcgccagcg cctggagccg tacgaaatgc cgtcgcttga cggcgtaaac
     1501 cgcgccagga tcgagaagtt caagctgcgc ctggccgatg cgctgaagca ggacggctgc
     1561 cagccttttg ccaggataat cgaggaatat caggaagaaa ccggggttcc ggccataagc
     1621 cttgcggccg cgctggcaag acagatgcag ggcagcgtgc cgttgttgat cgatcgcatg
     1681 cccgaaccgg cgatggatcc aaggccgtca acgaatcgtg gcccgcgtcc ggcatatggc
     1741 agcaggccgc cgcgtttcgg cgatcgcgcg tttgatcgcg cggcggatcg tcccggcgat
     1801 cgggctgcgt tccgtgaagg tgaccgggca ggcaaccgcg aaggcgaacg tcctttcgtc
     1861 cgtgaaggct cgcgtgccgc ccgcggattc ggagatcgtc ctgccgagcg ccagttcgag
     1921 aaccgtcaga tgtcgggtgg cgacaggccc ctgcgcaggc aggccgcgcc tggggaagac
     1981 cttccggccc gccgtccccc ggtcgatcgc gcacccgacc ccgggatgga tcgttaccgc
     2041 cttgaatgtg gccatacgca cggcgtgatg cccgggaaca tcgtcggggc cattgcgaac
     2101 gaggccggtc tgcggggcaa ggatatcggc cacatcaaca tttttgacga ttacagcacc
     2161 gtcgatctgc cggtcggcat gccggaccgg gtcttttcga tcctgaagaa gaccaaggtc
     2221 gcgaaccgct acctggactt gtccctgatg attcaatcga agctggaaaa gattgacgcc
     2281 cgcaggcacg aagaccgtcc cccgcgcgac cgtccgtccc gcaccccgga tcgtcgcacc
     2341 ccccccgccc gccgccgctg atctccagcg acctgagaat tgttttcagc aatgcattgt
     2401 gtgcggatga atttgctgct cgcgatacag gaaccgtgct tgaacaggca gaacccctga
     2461 gcgaagcgaa gaacctgtcg ggcgaatttt cattcgccct tacggatctg ggagcggcaa
     2521 acgacgggtg cgatggaagg cattttgctc gaccgcagcg ggcggatgta atccgcccgt
     2581 acaagggtag cgaaacttta ttgcggtgca taccggcttg ccaggaggaa cgcttggagg
     2641 ccaccaccac gactgaagca aagcgcgtac aacatggggg ccgagcgggt acccgtagcc
     2701 gggaagctct cgcaggcgcg cgacacc
//
LOCUS       DAOPPA010000451         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40546_length_2724_cov_3.942300, whole genome shotgun sequence.
ACCESSION   DAOPPA010000451 DAOPPA010000000
VERSION     DAOPPA010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40546_length_2724_cov_3.942300"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..614
                     /locus_tag="PKH54_11875"
     CDS             186..614
                     /locus_tag="PKH54_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOD00636.1"
                     /translation="MAPAAADGELKRLTKKQIAELKTELLAKRKALIVQARETMAVTE
                     EQEIREPDEVDQASSEYDQSFEYRMRDREKFLMRKIDKALQRLEEGEYDLCESCGNPI
                     GFKRLMARPEATMCIACKEEQERQEKQFQKKRQLRATIEF"
     gene            complement(632..1534)
                     /gene="miaA"
                     /locus_tag="PKH54_11880"
     CDS             complement(632..1534)
                     /gene="miaA"
                     /locus_tag="PKH54_11880"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD00637.1"
                     /translation="MICIMGPTASGKSDMAVELAVRLGGQIVNSDSMQVYRYLPIGTA
                     APDPSMYAAARHHLFEYRMPDDECDAGTWAREAATRIRAIADEGRIPIVVGGTFFWVR
                     ALFEGLSEIPAASAEARRSVAADIGARGSVAMHAELASIDPVTASRLEPADSQRIGRA
                     LEVWRTTGVALSEFHRREPVPAIEADVLKIKLCVERDVLYDRINQRLAKMVSRGLVDE
                     VRAVLEMGFSRDCRPLKSSSFEPVIRFLDGLIYLDAMSGEIAQGHRNYAKRQLTWLRR
                     ESGIALAAGDTGAALESAEKFLEA"
     gene            complement(1557..>2724)
                     /locus_tag="PKH54_11885"
     CDS             complement(1557..>2724)
                     /locus_tag="PKH54_11885"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00638.1"
                     /translation="HFVHRSGTRTVLDFPACQSLRDRASMVLGAQGGMYPFARQTGDA
                     AVEGVLSAPDAARGDASRTVILVNGRPVVDVAIRRVVVGAYSSLLPAGRFPVAVVAIT
                     LAPSEVDVNVHPQKHEVRFRRPREVQALVYEAMVAVIESTPWIAPGQAAHASGGSESF
                     REWPPVFAARPESFGPRDGIAGTPDQAVLPIPGLAPEGRSAPRYLGQIGNTVLVCEQA
                     GNLVLIDQHAAHERVNFNHLWNLLEAGEVPSDVLVFPEIVRLDAADMERFDVALAALH
                     RLGFDLEPYSGDSVAVRAVPAILKGRSVAEIVRQGVHAASEGPAVAGDALLRKIVSTV
                     ACHASVRAGDPLSPPEAAALLASMADQALSSYCPHGRQAVVVTPLAELLKQFDR"
BASE COUNT          567 a          865 c          855 g          437 t
ORIGIN      
        1 gcgcgcttgc tttgggagca agatgtcgca ggttcgaatc ctgtctcccc gacctgattg
       61 accgcatcgc ttgacgttga agttaccgcg gtcaataatc ttcccgatga ccttgaacgt
      121 gtcgggaacc gaatcatcgg gattcgcgcc tggggcgcgg cccgcattgc atccggaggg
      181 aaatcatggc accagcagcc gccgacgggg aactgaaaag actgacaaag aagcagatcg
      241 ccgagctcaa gaccgaactc ctggccaagc gcaaggcgtt gatcgtccag gcccgcgaga
      301 cgatggcggt taccgaggag caggaaatcc gcgaaccgga tgaggtcgat caggcatcgt
      361 ccgaatacga tcagtcgttt gaatacagga tgcgcgaccg cgaaaagttc ctgatgcgca
      421 agatcgacaa ggcgcttcag cgcctggaag agggcgagta cgacctttgc gaaagctgcg
      481 gcaacccgat cgggttcaag cggctgatgg cacggcccga agcaacgatg tgcatcgcgt
      541 gcaaggaaga acaggaacgc caggaaaagc agttccagaa gaagcgccag cttcgcgcga
      601 ccatcgagtt ctgattccgg gcgccggccc gtcaagcttc aagaaacttt tccgcggact
      661 caagcgcggc accggtgtcc ccggcggcaa gcgcgattcc ggattcccgt cgaagccacg
      721 taagctggcg ttttgcgtaa ttccgatggc cctgggcgat ctcgccggac atcgcgtcaa
      781 ggtagatgag cccgtcaagg aagcggatta ccggctcgaa actggacgac ttcaacggac
      841 ggcaatcccg ggaaaatccc atttccagga cggcgcggac ttcatcgacc agcccccttg
      901 acaccatttt cgcaagacgc tggttaatcc ggtcgtacag cacgtccctt tccacgcaca
      961 gcttgatctt caggacgtcg gcttcgattg ccggtaccgg ctcgcggcga tggaattcgg
     1021 acagtgcaac gccggttgtc cgccagacct ccagcgcgcg tccgatgcgc tgggaatcgg
     1081 ccggttcaag gcgcgatgcg gtcacgggat caatcgacgc gagttcggca tgcatcgcga
     1141 cactgcccct ggcgccgatg tcggccgcga ccgagcgcct ggcttcggcc gatgccgcgg
     1201 ggatttccga caggccctcg aaaagggccc tgacccagaa gaaggtgccg cccacgacga
     1261 tcggaatccg gccttcgtcc gcaatcgccc ggatgcgggt cgctgcctcg cgtgcccatg
     1321 tgccggcgtc gcattcgtca tccggcatgc ggtactcgaa aaggtgatga cgggccgcgg
     1381 cgtacatgga cgggtcgggc gcggccgtac cgatcggcag gtaccgatag acctgcatcg
     1441 aatcggaatt gacaatctgg ccgccaagcc tgacggcaag ctcgaccgcc atgtcggact
     1501 ttcccgacgc ggtcggaccc atgatgcaga tcacccgtgg tttcgttgac aacggttcac
     1561 ctgtcgaatt gtttaagcag ttcggcaagc ggcgtgacga cgaccgcctg acgaccgtgc
     1621 ggacagtacg atgacagcgc ctggtcggcc atcgacgcaa gcagcgcggc cgcttcaggt
     1681 ggggacaggg gatccccggc gcgaaccgat gcatgacacg cgaccgtcga gacgatcttg
     1741 cgcagcaggg cgtcgccggc aacggccggg ccctcggatg ccgcatgcac gccctggcgc
     1801 acgatttccg cgaccgaacg gcccttcagg atggccggga cggcccgcac ggcgaccgaa
     1861 tcgccggaat aaggctccag gtcgaacccc agccgatgca acgccgccaa tgcgacatcg
     1921 aaacgttcca tgtccgccgc gtccagcctg acgatctccg gaaagaccag gacgtccgat
     1981 ggaacctcgc ccgcctccag caggttccac agatggttga agttgacacg ctcgtgggcc
     2041 gcgtgctgat caatcagcac aagatttccg gcctgctcgc acacaaggac ggtgttgccg
     2101 atctggccca ggtatcgtgg ggccgaacgg ccttccggcg ccaatccggg aatcggcaga
     2161 acggcttgat ccggcgtgcc ggcgataccg tccctggggc cgaacgattc cggccgggcg
     2221 gcaaagacgg gtggccattc ccggaacgat tccgaaccac cggatgcatg cgccgcctgt
     2281 cccggggcga tccagggagt cgattcgatg accgcgacca ttgcttcata tacaagcgcc
     2341 tgcacctcgc gcggacgacg gaatcgaacc tcgtgcttct ggggatgcac gttcacatcg
     2401 acctcggacg gcgcaagcgt gatcgcgaca acggcgaccg ggaacctgcc ggcaggcagc
     2461 agcgaggaat atgcgccgac aaccacccgc cggatggcga cgtcaaccac gggacgcccg
     2521 tttacaagaa tcaccgtcct gctggcatcg ccgcgggccg cgtcaggtgc tgaaagaact
     2581 ccttcaacgg cggcatcacc ggtctgcctg gcaaagggat acatgccgcc ctgggcccca
     2641 aggaccatgg acgcacggtc gcgaagcgac tggcaggctg gaaaatcgag cacggtcctt
     2701 gtacccgacc ggtggacgaa atgc
//
LOCUS       DAOPPA010000452         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40734_length_2715_cov_3.274812, whole genome shotgun sequence.
ACCESSION   DAOPPA010000452 DAOPPA010000000
VERSION     DAOPPA010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40734_length_2715_cov_3.274812"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2523
                     /locus_tag="PKH54_11890"
     CDS             <1..2523
                     /locus_tag="PKH54_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD00639.1"
                     /translation="TNPDGFIAKDIDACDRMRADIMEITRMARSKAFRMAAASGALDH
                     DLLTRAAIDLCRREDRPDRLRRRFDALLVDEVQDSSPAQFSLFRAIENLPGDGRSLRT
                     FYVGDSRQSIYLFRGAEPAGLKELEQRSIQAGFPVEQLDTNYRSTPEMITAQKALFDR
                     AIHGHGDFPGLPGVTTIDHVQSNESRGRDELPPSAPYPEPIVMVTLEASTPNAPKWVP
                     ADFNNAALDVFCDRIMHAWNFEGRGDDTAAVLCSSWANAINARNRIRSILRAGNSGDA
                     FLDGSRELAKGIVAGDIRTVVSALWDSSDDIAWAGLWRLPMIGLSDGALAAFRAGSGL
                     LPADGGIRGLAWAASAQGLDPAVFGQRDFVAFARVRPVLEKARSEIGRVPTADVLEEM
                     AAALHWRSILLKGPGGMDAVGELEVILDWIRQAEADTVDPEAVVTMLDPKEAGEVPRV
                     ELYRGKKSVSCTTIHQAKGLKYDHVCVMSIGSPAGGGGFGGVSRGSMVLDGRELALLG
                     VSHDPEQGIHPRPDPVSRLAAGIDAKRSEEELLRLVYVAVTRAVRSVTFATGSHNVGI
                     HGALSDLWMADDVPMEGVKLVKLAAPNEIDTTIRAHVRAAAPFPVKPCPPAGWISVSP
                     SMAAEAWKTADADALDAMIQGLLPAVSVVYGADPLHPPKLDEMAGDDPISDSAWGNLV
                     HSWMESTGLRPGADVGAARAFLESRYGIHDDALAGWLAAILERLELHQPELLARLRSP
                     EATLHFEMPFAGVNTSTPDSPWFHAGRMDLLVTFPGKRAIVVDFKAGRRNPVPGANFV
                     KSAGLDEYMPQLEAYRQSLTAAGWTIETVGLLYMRSATWLSW"
BASE COUNT          512 a          772 c          899 g          532 t
ORIGIN      
        1 accaacccgg atgggttcat cgcaaaggat atcgacgcct gtgacaggat gcgggcggac
       61 atcatggaaa tcacccgcat ggcccggtcg aaggcgtttc gcatggccgc ggcatctggg
      121 gcgctggatc acgacctgct gacccgggcg gcgatcgatt tgtgcagacg cgaggatcgt
      181 cccgaccggc ttcgcagacg tttcgacgcg ttgctggtcg atgaagttca ggattccagc
      241 ccggcccaat tcagcctgtt ccgggcaatc gaaaatctgc cgggggacgg gcggtcgttg
      301 cgtactttct atgtcggcga ttccaggcag agcatctatc tgttccgcgg cgccgaaccg
      361 gcaggcctga aagaactgga acagagatca atccaggccg gcttcccggt cgagcagctc
      421 gacaccaatt atcgaagtac cccggagatg atcacggccc agaaggccct gttcgatcgg
      481 gccatccatg gccatgggga ttttcccggc ctgcccgggg ttaccacgat cgaccacgtc
      541 cagtcgaatg aatccagggg cagggacgaa ttgccgccat ccgcgccata cccggaaccc
      601 attgtgatgg ttaccctgga ggcatcaacg ccgaacgccc cgaaatgggt tccggccgat
      661 ttcaacaatg ccgccctgga cgtcttctgt gaccgaatca tgcatgcctg gaatttcgaa
      721 ggcagaggtg acgacaccgc cgccgtcctg tgttccagct gggcgaatgc catcaacgca
      781 cgcaaccgga tcagatcgat tctgcgggcc gggaattccg gcgatgcgtt tctggacgga
      841 agtcgtgaac ttgcaaaggg gattgtcgcg ggcgacatca gaacggttgt gtcagcgctg
      901 tgggacagtt ccgatgatat tgcctgggcc ggtttgtggc ggcttccgat gatcggtctt
      961 tcggacgggg ctttggccgc tttcagggcg ggtagtggcc ttcttccggc ggacggcggc
     1021 attcgggggc tcgcctgggc tgcgtcggcc cagggccttg atccggcggt tttcggtcag
     1081 cgggattttg tcgcctttgc gcgggtcagg cctgttctgg aaaaagccag gtccgaaatc
     1141 gggcgcgttc ccaccgccga tgtcctggaa gaaatggcgg cggcgctgca ctggcgaagc
     1201 atccttctga aaggccctgg cggcatggat gccgtcggcg aactggaagt gatccttgat
     1261 tggatcaggc aggcggaagc ggacaccgtg gatcccgagg ccgtcgtcac gatgcttgac
     1321 ccgaaggaag ccggcgaggt cccgcgagtc gaactgtacc gaggcaagaa aagcgtcagt
     1381 tgcacgacga ttcaccaagc caaggggctg aaatacgacc acgtctgcgt gatgtcgatt
     1441 gggagtcctg ccggaggtgg tggcttcggc ggcgtatcca ggggttcgat ggtgctggat
     1501 ggccgggaac tggcgcttct tggcgtcagc catgatcctg aacaggggat tcacccgcga
     1561 ccggatccgg tatccaggct ggcagccggg atcgatgcga agcgcagcga agaagagttg
     1621 ctgcgtctgg tatatgtggc cgtcaccagg gccgtcaggt cggtaacttt tgcaactgga
     1681 tcccacaatg ttggaattca tggcgccttg tcggatctgt ggatggccga cgatgtgccc
     1741 atggaaggcg tcaagctggt taagttggcg gcaccgaatg aaatcgacac aaccatcagg
     1801 gcacatgtca gggcggccgc gcctttccca gtgaaaccat gtcccccggc cggatggatt
     1861 tccgtgtcgc cgtcgatggc ggccgaggca tggaagacgg cagacgcgga tgcacttgat
     1921 gccatgatcc aggggctgtt gcccgccgtg agtgtggttt acggtgccga cccgcttcat
     1981 cccccgaagc tggatgaaat ggcaggggat gacccgattt ccgattcggc gtggggtaac
     2041 ctggttcact cctggatgga atccacgggc ctgcgaccag gggccgacgt cggggccgcc
     2101 cgcgccttcc tggaatcgcg gtacggcatt catgacgatg cgctggccgg ttggcttgcg
     2161 gcaatccttg aacggcttga actgcaccag cccgaacttc ttgcccggct gaggtcaccg
     2221 gaagcgacgc tgcacttcga gatgccgttt gcgggcgtca atacatcgac gccggacagc
     2281 ccatggttcc acgccggccg catggacctg ctggtgacct tccccggaaa gcgggcgatt
     2341 gtcgtcgatt tcaaggcggg caggcgaaac ccggtgcccg gcgccaactt tgtgaaatcg
     2401 gcgggcctgg atgaatacat gccccagctt gaggcctacc gtcagtccct gaccgcggcc
     2461 ggctggacca tcgaaacggt cggcctgctt tacatgcgca gcgccacctg gctgtcgtgg
     2521 tgatttgccc ggatgaatgc cgatgttgcg acggtcggga agggttgact ggcggttcat
     2581 tgttgaccga cacttgtgtc gggcgtaatg ctgggtcgcc gttgatgacg gcgccagatt
     2641 tcaggaaaaa cgatggcggt tgccagagca cccagaaaca ggacgttgac tctgacggac
     2701 gaacagcgca ggagt
//
LOCUS       DAOPPA010000453         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40773_length_2713_cov_3.375470, whole genome shotgun sequence.
ACCESSION   DAOPPA010000453 DAOPPA010000000
VERSION     DAOPPA010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40773_length_2713_cov_3.375470"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKH54_11895"
     CDS             complement(<1..627)
                     /locus_tag="PKH54_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOD00640.1"
                     /translation="MAYKICPNCGTQQPEDYMFCAECGTKLGDVPVAGAEPEAAWAQP
                     QEPVRVVPPAQPVAPSWSAPRPTPAPVRPAREPAPVQQPAARPAPAPVPAAAPRPAPV
                     PQTPAAPIARPGRRLAWSLVHLSRAGGTNVPYQVPEEGMFVGRQGCGICFPDDRTLSE
                     RHAMVSIGDRGLVVEDAGSVNGLFQKITTDHILTDGDVFVCGDSVFRFS"
     gene            complement(681..1802)
                     /locus_tag="PKH54_11900"
     CDS             complement(681..1802)
                     /locus_tag="PKH54_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD00641.1"
                     /translation="MKVEPIFLVDQDSPFAELLFSGLGRVEYFGDSDPTSRPDLLESA
                     DVLSVRSSTRVDSGLLSRCNNLKVIVTPVVGTDHIDTNAVAELSARLGRVIPVFNAAG
                     STAEAVADWTIGAIFKSLEGAPASVGIVGVGNIGRQVARRLDLLGITYLTCDPGRMDE
                     PEFRHTPIEEVLGCQVVTFHVPMTRSGQSPWPTAGMISGQLCEDARHCGCRLFVNSSR
                     GGIVVPDVFLMAGDRRPALACDVFLDEPAPAPAAIAACTVATPHLAGSGRYGRARAAM
                     MVRDKVRAALDLPSQPLDPGLFGLPGRPEIRGLPGCISTESGMTRFLGEIDILSGTSQ
                     SASFRSEYISAAASDRARVFTSFRMAGTRTEPLWIGSCN"
     gene            complement(1824..2195)
                     /locus_tag="PKH54_11905"
     CDS             complement(1824..2195)
                     /locus_tag="PKH54_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00642.1"
                     /translation="MEASEPIFKCYNCGEELVFDVKIGRRDMCPNCYAYLHCCYNCKF
                     WDKNVHNQCTENQGEFIRDRAEGNFCLYFTFKPVPGTEGITEEQKAKAKIESLFGGPK
                     VETSPRTADDAKARLEKLFSK"
     gene            2419..>2713
                     /locus_tag="PKH54_11910"
     CDS             2419..>2713
                     /locus_tag="PKH54_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00643.1"
                     /translation="MLLAIHGSDKVMENMVNISFPVTEIRGEKASGSGIATRLGLDGM
                     ELQSKDVPPGRFAWIGFTLPDTGRTVKVLGEIMGIRRDGTIDSITVRFKHLFPN"
BASE COUNT          551 a          783 c          844 g          535 t
ORIGIN      
        1 actgaacctg aaaacggagt cgccgcatac gaaaacatcg ccgtcggtca gaatatggtc
       61 ggtcgtgatc ttctggaaaa ggccgttgac gctgccagcg tcctcgacga caagtccgcg
      121 gtcgccgatc gaaaccatcg catgcctttc agacagcgtc ctgtcatccg ggaaacagat
      181 gccacagccc tggcggccaa cgaacattcc ttcttccgga acctggtacg gaacgttggt
      241 tccgccagcc ctggaaaggt gaaccagcga ccaggccagg cgcctgccgg gacgcgcgat
      301 tggcgccgcg ggcgtctgtg gaaccggcgc gggacgtggc gcagcggcgg gaacaggtgc
      361 cggggccggt ctggcggcgg gctgctgaac aggggcaggt tcgcgcgccg gccgcactgg
      421 tgccggggtg gggcgcggcg ccgaccatga aggggcaacc ggctgcgccg gcggcacgac
      481 gcgaaccggc tcctgtggct gggcccaggc ggcttcaggc tcggcccccg ccacgggaac
      541 atcgccaagc ttggttccgc actcggcaca gaacatataa tcttcgggct gctgcgttcc
      601 gcaattcggg cagattttgt aagccatggg ttcagtccct tcaagaagcc atcaatttat
      661 agatgcaatc atgggcagtg tcaattgcag gaaccgatcc agagtggctc cgtccgggtg
      721 ccggccatcc tgaaagatgt gaacacacgg gctctgtcgg atgccgccgc ggaaatgtat
      781 tccgacctga atgatgctga ctgtgaagtg cccgaaagaa tgtcgatttc accaagaaat
      841 cgcgtcatgc cggactcagt tgaaatgcag ccgggtaacc ctcggatttc gggccgcccg
      901 ggaagcccga aaagtccggg atcaagcggt tgggacggca ggtcaagtgc ggcgcgaacc
      961 ttgtcgcgga ccatcatggc ggccctggcg cgtccatatc ggccgcttcc cgcaaggtgc
     1021 ggtgttgcaa ccgtgcatgc agcgatggcg gcaggggcag gagccggttc gtcaaggaag
     1081 acgtcgcagg caagggcggg gcgcctgtcg ccggccatca ggaacacatc cgggacgacg
     1141 attccgccac gtgaactgtt gacaaaaagc cggcagccgc aatgtctggc atcttcgcaa
     1201 agctgcccgg aaatcattcc ggctgttggc catggtgatt gcccggaacg tgtcatcgga
     1261 acgtggaacg tcacgacctg gcagcccagc acttcctcaa tcggggtgtg ccggaattca
     1321 ggttcgtcca tccttcccgg atcgcatgta aggtaagtga ttccaagcaa atccagtctg
     1381 cgggccacct ggcgcccgat gttgcccacg ccgacgatcc cgaccgaagc cggggcgcct
     1441 tcaagcgact tgaagattgc accgatggtc cagtccgcca cggcctcggc ggttgaaccg
     1501 gccgcgttga agaccgggat cactcgcccc aggcgcgccg acagttcggc aaccgcgttc
     1561 gtgtcgatat ggtcggtgcc gacgacgggg gtcacgataa ccttcaggtt gttacagcgg
     1621 gaaagcaatc ccgaatcgac cctggttgaa gaccttactg acagcacatc ggcggactca
     1681 agcagatccg ggcgggatgt cggatccgag tccccgaaat actcgacgcg gccgagtccc
     1741 gaaaaaagaa gctccgcgaa ggggctgtcc tgatcgacaa ggaagatggg ttcgactttc
     1801 atgctgacat tgtcgccccg gaattactta ctgaacagct tttccaggcg ggcctttgcg
     1861 tcgtcagcgg tgcgcgggct ggtttcaacc tttggtcccc cgaacaacga ctcgatcttt
     1921 gcctttgcct tctgttcctc ggtgatgcct tcggttccgg gaaccggctt gaaagtgaag
     1981 taaaggcaga agttgccttc ggcgcggtcg cggatgaact cgccctggtt ctcggtgcac
     2041 tggttgtgga cgttcttgtc ccagaacttg cagttgtagc agcagtgcag gtacgcgtaa
     2101 cagttcgggc acatgtcgcg ccgaccgatc tttacgtcga agacaagttc ttcgccgcag
     2161 ttgtaacact tgaagattgg ttcgctggct tccatcgggc gattccttca cagtcgcaac
     2221 cacgggccat tcccgggtga cggcggatct taatgctttc cggtcaatcc gtaaatcgcc
     2281 agttgtcgga ttcgccgatt cgcggtgttt tcggcaggat cggcacgacc ttcaaaaaaa
     2341 tcctgtcgtc tttttggcat tgatcgcaaa cctgatttac ggtgtgttcg ggatcgacgt
     2401 cggaatcgga aggcgtcgat gcttttggcg attcacggga gcgacaaggt tatggaaaac
     2461 atggttaaca tcagtttccc ggttaccgaa atcaggggtg aaaaggcgtc tggctcggga
     2521 atcgccacac ggctgggact cgatgggatg gaactccagt caaaggacgt tccgcccggc
     2581 aggttcgcct ggatcggctt cacgcttccg gataccggca ggacggtgaa ggttcttggc
     2641 gaaatcatgg gaatccgccg cgatggaacc atcgacagca taaccgtgcg cttcaagcat
     2701 cttttcccga acg
//
LOCUS       DAOPPA010000454         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40885_length_2707_cov_3.239819, whole genome shotgun sequence.
ACCESSION   DAOPPA010000454 DAOPPA010000000
VERSION     DAOPPA010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40885_length_2707_cov_3.239819"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="pilM"
                     /locus_tag="PKH54_11915"
     CDS             <1..471
                     /gene="pilM"
                     /locus_tag="PKH54_11915"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOD00644.1"
                     /translation="VLSGASIAFTRDISIGGSRLTTEIQRQFNVSWEEAEAFKTGAES
                     SLSSSTIARDVNKLADKVCDSIVTEIQRSLDFYVATSVNSDISKVYLTGGSAQLPALI
                     RTLERRMEVPVELVNPFRNIAVDARKFDIDLLQRAAPVAGVSVGLALRRAGDSE"
     gene            468..1352
                     /locus_tag="PKH54_11920"
     CDS             468..1352
                     /locus_tag="PKH54_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD00645.1"
                     /translation="MIRINLIGRKVKQKKSHGSYQVLIYLALLGVACVVLFLWHQSVN
                     DELEGSKKRAREAAEKVENLMRVKQMWEAWQAEKADLDRQTAVFNALQADQIGPAMAL
                     QYLSYALTRLTDEAAQGEEAREQELAGWNPKWDPSRIWIRSIEIKNGVAEFKGEAIDH
                     EDVAEFYRRLESSNLFRNIAPGTQRRRIHEELQIKFVEFQVRAEVSFVESALAWRDAE
                     DRAARQAAEQQLAAQAAVDREAADDGAGPAGEPAGEPGSEPEGPAPTEPQAAPATPEG
                     GTAPAEGAQQPAEKGISE"
     gene            1349..2056
                     /gene="pilO"
                     /locus_tag="PKH54_11925"
     CDS             1349..2056
                     /gene="pilO"
                     /locus_tag="PKH54_11925"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOD00646.1"
                     /translation="MNKFFDLPLIQRILIVAIALAVVTGGGFYLLILPTTQAISREQI
                     KLKNTMNEYSKLREYDAPEFKQEMDRQKAEAVKQREAYAKMLPREDELPDLIDALKRE
                     ADNSGLALTRFEPNKEGEVGDGYRGLAFDVEMIGSYHQIVTFMNNLAAPSKRIINAKN
                     LNIQLVPSDQLQKSAGDVGMLRVIMERETARGLTPTEKYAKAVLLFDDIAKRRLLKVT
                     FTAMAYVYTGERAPTAP"
     gene            2069..2668
                     /locus_tag="PKH54_11930"
     CDS             2069..2668
                     /locus_tag="PKH54_11930"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOD00647.1"
                     /translation="MKRNLLMLFVVAVAFTACGSDSPMLSVITPTQEDLNKAANKVLG
                     GVPQAAQAAEAVPAAVTGPVGIRKYYFDYLHTPVEAKINPFQSNMAAFTPTVEIEADV
                     VAEETGPVTPLQYYDTDSYKLVLIMSGTAIPKALVSDPQGKSYVIMVDTAIGNRQGRV
                     VSITANEVRIDEPGFPPVVKTLGTDREDMIKELQSVQEF"
BASE COUNT          602 a          767 c          835 g          503 t
ORIGIN      
        1 gtgctttccg gtgccagcat cgccttcacc cgggatatca gcatcggtgg cagccggctc
       61 acgaccgaaa tccaacgaca gttcaacgtg tcgtgggagg aagccgaagc attcaagacc
      121 ggcgccgaat cctcgctttc gtcatcgaca attgcccgcg acgtcaacaa gctggcggac
      181 aaggtgtgcg actcgatcgt aacggaaatc cagcgatcgc ttgatttcta tgtcgcgacg
      241 tccgtaaaca gcgacatcag caaagtgtat ctgaccgggg gctcggcgca gctcccggcc
      301 ctgatccgaa cgcttgaacg caggatggaa gtgccggttg aactggtcaa tcccttcagg
      361 aacatcgcgg ttgacgccag gaagttcgac atcgaccttc tccagagggc tgcgccggtt
      421 gccggtgtca gcgttggtct tgctttaagg cgagccgggg attcggaatg attcgcataa
      481 accttattgg ccgcaaggtt aaacagaaga agtcccatgg cagctaccag gtgctgattt
      541 acctggccct gcttggggtc gcctgcgtcg tgctgttcct gtggcaccag agcgtgaacg
      601 acgaactgga aggttcgaag aagcgcgccc gggaagccgc ggaaaaggtg gaaaacctta
      661 tgcgcgtcaa gcagatgtgg gaagcatggc aggccgagaa ggccgacctg gatcgccaga
      721 cggcggtttt caacgcgctt caggcggacc agatcggccc ggcgatggcg ttgcagtacc
      781 ttagctatgc attgacccga ctgaccgacg aggcagccca gggtgaggag gccagggagc
      841 aggagcttgc aggatggaat ccgaagtggg atcccagtcg aatctggatt cgatcgattg
      901 aaatcaagaa cggtgtcgcg gagttcaagg gcgaggcaat cgatcatgag gacgttgccg
      961 agttttaccg ccggctcgag agttccaacc ttttcaggaa catcgcgccg ggaactcagc
     1021 gcaggcgcat ccacgaggaa ctccagataa agttcgtcga attccaggtc agggccgagg
     1081 tttccttcgt cgaaagcgcg cttgcatggc gtgatgccga ggatcgggcg gcacgtcagg
     1141 cggcagagca gcagttggcg gcacaggcgg cggttgatcg cgaggcggcg gatgacggcg
     1201 cagggcctgc cggcgagcct gcgggtgaac cgggatctga acctgaaggt ccggcaccga
     1261 cggaacccca ggccgcaccg gccacccccg agggtggcac cgcaccggcc gaaggcgcac
     1321 agcaacccgc ggaaaaggga atatcagaat gaacaagttt tttgatcttc cgctgattca
     1381 gcgaatcctg atcgttgcga ttgcgcttgc ggtcgtcacg ggcggcggct tttacctgct
     1441 gatccttccg accacccagg cgatcagccg cgaacagatc aagctcaaga acacgatgaa
     1501 tgagtactcg aagctgcgtg aatacgacgc gcccgagttc aaacaggaaa tggatcgcca
     1561 gaaggccgag gccgtcaagc agcgcgaggc ttacgcgaag atgctgccgc gcgaggacga
     1621 gcttcccgac ctgattgatg ccctgaagcg cgaggccgac aactccggcc ttgccctgac
     1681 caggtttgag ccgaacaagg aaggcgaggt cggcgacgga taccgcggac tggcgttcga
     1741 tgtcgagatg atcgggtcgt atcaccagat tgtgactttc atgaacaacc tggcagcgcc
     1801 gtccaagcgc atcatcaatg cgaaaaatct gaacattcag cttgtcccgt ccgaccagct
     1861 tcaaaaaagc gccggcgacg tgggcatgct tcgtgtcatc atggagcgcg agacggctcg
     1921 cggacttacg ccaacggaaa agtacgcaaa ggccgtgctg ctgtttgacg acattgccaa
     1981 gcgccggctt ctgaaagtga ccttcaccgc gatggcctat gtatataccg gtgaacgcgc
     2041 cccgacggcg ccgtgattgg gggtaaacgt gaagcgtaac ctgttgatgc tttttgtggt
     2101 tgccgtcgcg ttcactgctt gtggaagcga cagcccgatg ctttcggtga tcacgccgac
     2161 ccaggaagac ctgaacaagg ccgcaaacaa ggttcttggc ggggttcccc aggcagcgca
     2221 ggccgccgag gccgttcccg ccgcagttac cggtcccgtc gggatccgca agtactactt
     2281 tgattacctg cacacgccgg tcgaggccaa gatcaacccg ttccagtcga acatggccgc
     2341 gttcacgccc acggtcgaga tcgaagcgga tgtcgttgcc gaggagactg gcccggtaac
     2401 cccccttcag tactacgata ccgattcgta caagctggtt ctgatcatgt cgggcaccgc
     2461 gattccaaag gcgctggtca gcgatccgca gggtaaatcg tacgtcatca tggtggatac
     2521 ggcgattggc aaccgtcagg gccgggttgt ttcgattacc gccaacgaag tccgaattga
     2581 tgaacctgga ttcccgccgg tcgtcaagac gcttggcacc gatcgcgaag acatgatcaa
     2641 ggaacttcag tccgtccagg aattctgatc cagcgagtcc catttccaga tatcatcaag
     2701 ccggttg
//
LOCUS       DAOPPA010000455         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_40899_length_2706_cov_4.556017, whole genome shotgun sequence.
ACCESSION   DAOPPA010000455 DAOPPA010000000
VERSION     DAOPPA010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40899_length_2706_cov_4.556017"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1324
                     /locus_tag="PKH54_11935"
     CDS             233..1324
                     /locus_tag="PKH54_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00648.1"
                     /translation="MAFRNQKTDRIRRLAQCVNLAILALVLTACSPKMTQLTMEPLEF
                     AGIRTADGVKVQTMDPELLFNDGAEAFKAGDFDQAARFFSVVCERFPDSSFGPPALFN
                     LGLARLRHGRPADAATAFRRFLEKFPGEGDIEQVRLRLAEALLESGDWAAAESVLGAM
                     MVAAAPRPAVELEIRAGLARALRKQGRLKESMDQVDAAMQVYDRHLVDPDIKGNFHAA
                     MASFEGGEVWHDLFASIKFILPKDRMEKDLSDKGTMFIKAQAGYMRTVRVGNIFWASK
                     AGVRIGQLYEEFYDDIMKAEVPPELDDHQLGQYMAELRRQARPLLSKSIELYERNMKV
                     ARSYGAKDEWFADMKARLDRLKELLKGIE"
     gene            complement(1391..2251)
                     /locus_tag="PKH54_11940"
     CDS             complement(1391..2251)
                     /locus_tag="PKH54_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD00649.1"
                     /translation="MNTVNAELSFRTLGSGSCGNMAVVRSGSTTILIDAGLGSQRLIR
                     ERLAECGVEPLQIRAALISHGHCDHLAFAGLKFCCDMGIPILGQIDTLNKATEMMVNK
                     TGRLPDHGVLQVLAPGTRIVIGDIDVTTFFVPHDVPTMGFVLRPAGMEFPKVTVATDI
                     GRTGDDLIGHFMDADAIFIESNYNENMLRHSARGLRDRHRVQSATGHLGNIDAGRFIG
                     RVYNLSARKPSVVTLMHLSDDHNTPACAIEDFKSASGLSHPECFCLSTAPRNGVGPEI
                     RVVREHCRKS"
     gene            complement(2248..>2706)
                     /gene="truB"
                     /locus_tag="PKH54_11945"
     CDS             complement(2248..>2706)
                     /gene="truB"
                     /locus_tag="PKH54_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOD00650.1"
                     /translation="DKEYVMTVRFACETDSCDLTGVPLDLPRGIVPSTGAILDALKLF
                     TGSIMQEPPVFSALKLHGKPLYWYARQGNPVAADARPVRIDSIDFVEMIGPDAVFVVK
                     CGKGAYMRSLGRDIGRALGCPAHLVALRRTVVGPYNVSDAWPLWRLQNFR"
BASE COUNT          577 a          803 c          804 g          522 t
ORIGIN      
        1 tccattgttc tgaacgcgac cgatgaaaac ttcgtcggaa gcgaaaaacc actccttctc
       61 ggtaccgccc ttctcgatga ccgttacctt aagcattgga gtccccggtt taatgccgtt
      121 gctgattttt ccaaagccgg gggtacatgt caaactgaaa gccggtacaa tcgacacccg
      181 gaagaatata tatcggctga aaagtaccga taaattttaa gggacatccg ttttggcatt
      241 tcgaaaccag aaaaccgacc gaatcaggcg ccttgcgcag tgcgtcaatc tggcgatcct
      301 ggcgcttgtt ctgacagcct gctcgccgaa aatgacccag ttgacgatgg aaccgctgga
      361 attcgccggg atccggactg cggacggcgt caaggtccag acaatggatc cggaactcct
      421 tttcaacgac ggggccgaag ccttcaaggc cggtgatttt gatcaggccg ccaggttctt
      481 ctcggtggtg tgtgaaagat tccccgactc cagcttcggg ccgcctgccc ttttcaacct
      541 ggggctggca aggcttcgcc acggccgccc ggctgacgcc gcaacagcct tcaggcgctt
      601 tctggaaaag tttcccggag agggtgatat tgaacaggtg cgactccgcc ttgccgaagc
      661 ccttctggag tcaggagact gggccgccgc cgaatcggtt ctcggggcaa tgatggtcgc
      721 ggccgcgcct cgaccggccg tcgagctgga aatccgcgcc ggcctggcgc gagcgcttcg
      781 caagcaggga cgcctgaagg aatccatgga ccaggtcgat gccgccatgc aggtctatga
      841 caggcacctt gtcgatcccg acatcaaggg aaacttccat gccgcaatgg catcctttga
      901 aggcggggag gtctggcacg acctgtttgc ctcgatcaag ttcatccttc ccaaggatcg
      961 gatggaaaag gatctttccg acaagggaac gatgttcatc aaggcccagg cgggctacat
     1021 gcggacggtg cgcgtcggca acatattctg ggcctcgaag gccggcgtcc gcatcgggca
     1081 gctatacgag gaattttacg acgacatcat gaaggccgag gttccgcccg aacttgacga
     1141 tcaccagctt gggcagtaca tggccgagct tcggcgccag gcacgccctc ttctgtccaa
     1201 gtcgattgaa ctgtatgagc gcaacatgaa ggttgcccgt tcctacgggg cgaaggacga
     1261 atggttcgcc gacatgaagg cccgccttga ccgcctgaag gaactgctga aaggcatcga
     1321 ataggatcca ccggatcaga ccgcgcagcc acccgccacg gatggtacag tacgattgaa
     1381 tcaagatggt tcaagatttc cggcagtgct cccggacgac ccggatttcg gggccgacgc
     1441 cgtttcgcgg cgcggtggac aggcagaagc attccgggtg ggacaggccg ctggccgact
     1501 tgaaatcctc gatggcgcag gccggggtat tgtgatcatc gctcagatgc atcaacgtta
     1561 cgactgacgg cttgcgggcg ctgagattgt agacgcggcc gataaaccgg cccgcgtcga
     1621 tgttaccaag gtggccggtc gccgactgca cgcggtggcg atcccgcaat cccctggcgc
     1681 tgtgacgcag catgttttcg ttatagttgg actcgatgaa tatggcgtcg gcatccatga
     1741 agtgaccgat caggtcgtct cccgtccgac cgatatcggt ggcgacggtc accttcggga
     1801 actccatccc ggccggccgc aggacgaagc ccatcgtcgg cacgtcgtgt ggcacgaaga
     1861 acgtcgtcac gtcgatatcc ccgatgacta tcctggtacc gggcgcgaga acctgcagga
     1921 cgccgtgatc cggcagacgg ccggtcttgt tgaccatcat ttcggtggcc ttgttcagcg
     1981 tgtcaatctg gcccaggatc ggaatgccca tgtcgcagca gaacttcagc ccggcaaaag
     2041 ccaggtgatc gcaatggccg tggctgatca acgcggccct gatctgaagc ggttcaacac
     2101 cgcattcggc cagccgttcc cgaatcaatc gctgggatcc aaggccggca tcgataagga
     2161 tggtggtcga accggatcgg accacggcca tgttgccgca tgaaccgctt ccaagcgtcc
     2221 tgaatgagag ttccgcgttc acggtattca ccgaaaattc tgcagccgcc acaggggcca
     2281 ggcgtcagac acgttgtagg ggccaacgac cgttcgacgc aatgcaacca ggtgcgccgg
     2341 acagccgagc gcccgaccga tgtcacgtcc cagggatcgc atgtaggcgc ccttcccgca
     2401 ctttacgaca aaaacggcgt ccgggccgat catttcgacg aaatcgatcg aatcaatccg
     2461 aaccggcctg gcatcggccg cgaccgggtt tccctgcctg gcgtaccagt aaagcggctt
     2521 gccgtgaagc ttcagcgctg aaaacactgg gggctcctgc attatcgaac cggtaaagag
     2581 cttcagcgcg tccaggatcg cgcccgtgga tgggacgatt ccgcggggaa gatccagcgg
     2641 cacgccggtc agatcgcacg aatcagtttc gcaggcaaaa cgaaccgtca tcacgtattc
     2701 cttgtc
//
LOCUS       DAOPPA010000456         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_41475_length_2678_cov_2.810904, whole genome shotgun sequence.
ACCESSION   DAOPPA010000456 DAOPPA010000000
VERSION     DAOPPA010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41475_length_2678_cov_2.810904"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKH54_11950"
     CDS             <1..554
                     /locus_tag="PKH54_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00651.1"
                     /translation="KFELSDYLYDDFAKLPIPSTLKPAVLKKALTDKAEAQQKAEGSF
                     NEVLDFKSGPWSAGALFKIGLLYYTFKEELLNLPIPEDLPEEYIVEYQGVIDQIVEPV
                     EAKAMRAFEKALNLAHEKKVYNNFSKSCAEYAAKVNQDTFPVAGDELVKSDRIKDSVT
                     STSFIKSLKRGDVEIKYIEEAAK"
     gene            551..2044
                     /locus_tag="PKH54_11955"
     CDS             551..2044
                     /locus_tag="PKH54_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00652.1"
                     /translation="MRKVFVMITAGVVCAGIAACGPVSKQTRKDGDKTQVQAAAKPAP
                     AKVETKEDLSYREIIQREFKYVEGVKEDAQDAFGQGVLFLHEFPPMYDRAVESLQKAI
                     QIDPGFAEAYINLAQIYERQRKTDDAVRLYQQAADKTDRKLDARAYIGKVYLGLAKKA
                     RDFGNFAEADRQELAAKQIFDEVIAEDAENIQANNSLALYWLFKGDTKTAESYVQKVL
                     LKAPEDVVALNTRGLINLQAGNLKIAKWVFEQKALRFDPNSIEALTNLGITYLRMGNT
                     PMGVTSFEKALQIDPNNFEARMNVAAIYLNYLHYNAAREMYEEALKMVPDSVEAMIGL
                     GSCYAGLLDPQKAVDTWLKGMRIAPKNTDLYLRVAKMYETKLNSLEKAVEYYQKYLEV
                     AQAPENDPIRGRIPVLQDMIKNGGMLMPPEEPMMPEETPPADGAVAPAVEPAPEAAPA
                     VEPAPAPEAAPAVEPAPAVEPAPAPAPEAAPAVEPAPEAAEAPVAAE"
     gene            2111..2368
                     /locus_tag="PKH54_11960"
     CDS             2111..2368
                     /locus_tag="PKH54_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00653.1"
                     /translation="MKKLVFAIVFAAVFAGVMPVAIAQTSGGVKSKFYDFSDQLIDGD
                     IKKPTTLFTDARQKVKFDRLLRLKKSFVEPMLETAKETTFK"
BASE COUNT          616 a          752 c          796 g          514 t
ORIGIN      
        1 ccaagtttga gctttcggat tacctgtatg acgacttcgc gaagctgccg attccctcga
       61 cactgaagcc cgccgtcctg aagaaggccc tgaccgacaa ggccgaagcc cagcagaagg
      121 cggaaggctc gtttaacgaa gtcctggact tcaagagcgg tccgtggtcg gccggcgcat
      181 tgttcaagat cggtctgctg tactacacct tcaaggaaga actgctgaac ctgccgattc
      241 cggaggatct gccggaagag tacatcgtcg agtaccaggg cgtcatcgac cagatcgtcg
      301 agcccgtcga ggccaaggcg atgcgcgcgt tcgagaaggc gcttaacctg gcgcacgaaa
      361 agaaggtcta taacaacttc tccaagtcct gcgctgaata cgcggctaag gttaaccagg
      421 atacgttccc ggttgccggc gatgaactgg tgaagtccga caggatcaag gattccgtca
      481 cttccaccag cttcatcaag tcactgaagc gcggcgacgt cgaaatcaag tacatcgagg
      541 aggccgcgaa atgaggaagg ttttcgtaat gatcaccgcc ggcgtcgtgt gtgccggtat
      601 tgcggcttgc ggtcccgtct cgaagcagac caggaaggac ggcgacaaga cccaggtgca
      661 ggcggccgcg aaaccggccc cggccaaggt cgagacgaag gaagatctgt cttatcgcga
      721 gatcatccag cgtgagttca agtacgtcga aggcgtcaag gaagacgccc aggatgcgtt
      781 cgggcaggga gttctgttcc tgcacgagtt ccctccgatg tacgaccgcg cggttgagtc
      841 tctccagaag gcaatccaga ttgatccagg tttcgccgag gcgtacatca acctggccca
      901 gatctacgag cgccagagaa agaccgacga tgccgtaagg ctgtatcagc aggccgccga
      961 caagacggat cgcaagcttg atgcacgcgc gtacatcgga aaggtctatc ttggcctggc
     1021 caagaaggcg cgtgacttcg ggaatttcgc cgaggctgat cgccaggaac ttgccgccaa
     1081 gcagatattc gacgaagtca tcgccgagga cgcggaaaac attcaggcca acaactcgct
     1141 ggcactttac tggcttttca agggcgacac caagacggcc gaaagctatg tccagaaggt
     1201 tctcctgaag gcgcccgagg acgtcgtcgc gctgaacacg cgcggtctga tcaatctgca
     1261 ggcaggcaac ctgaagatcg cgaagtgggt tttcgagcag aaggcgcttc gtttcgaccc
     1321 gaactcaatc gaggcgttga cgaaccttgg gatcacgtac cttcgcatgg ggaacacccc
     1381 gatgggcgtg acgtcgtttg aaaaggcgct gcagattgat ccgaataact ttgaagcgcg
     1441 catgaatgtc gcggccattt atctgaatta tcttcactac aatgcggccc gtgagatgta
     1501 cgaggaagcg ttgaagatgg ttccggacag cgttgaagcg atgatcggcc ttggctcgtg
     1561 ctatgccggg ctgcttgatc cccagaaggc cgttgatacc tggctgaagg ggatgaggat
     1621 tgcaccgaag aatacggatc tgtacctgcg cgtcgccaag atgtacgaga cgaagctgaa
     1681 cagtctggaa aaggcggtcg agtactacca gaagtatctt gaagtcgccc aggccccgga
     1741 aaacgatccg atccgcggac gtatccccgt tcttcaggac atgatcaaga acggcggaat
     1801 gttgatgccg cctgaagagc cgatgatgcc agaagagacg ccaccggccg atggcgctgt
     1861 ggccccggcg gttgaaccgg ctcccgaagc ggctccggcg gttgaaccgg ctccggcacc
     1921 tgaagcggcc ccggccgttg aaccggcccc ggccgttgaa cccgcgcctg caccagcacc
     1981 tgaagcggct ccggcggttg aaccggctcc tgaagcggcc gaagcgccgg ttgcggcgga
     2041 gtgaagttga tcgaatcggg tcgcgtgcgt atcattgacg cgctgacccg gattttgggg
     2101 gtaacgtaag atgaagaagc tcgtattcgc gatcgttttc gcggccgtgt tcgccggcgt
     2161 gatgcctgtt gcaatcgccc agacttccgg cggcgtcaag tcgaagttct atgacttttc
     2221 cgaccagttg atcgacggtg atatcaagaa gccaacaacc cttttcacgg acgcccgcca
     2281 gaaggtcaag tttgaccgcc tgctgcgcct gaagaagtcc tttgtcgagc cgatgctcga
     2341 aaccgccaag gaaaccacct tcaagtaggg gcgaatgaga attcgcccgc atccgtgaat
     2401 gaaaattcgc ccgttcccgt cattgtgcga acggattacc gctgcgtcct tgtacgggcg
     2461 gattacatcc gcccgctgcg aaccgcaggg gagaatgaaa cctcgcccat cgccaacaga
     2521 acccgtcaga aagtgtcctt acgtcggagt taccgggaaa ccggcatgac atcgatcttc
     2581 aactcttcgt ttacgaccgg gaagaacaga ttgattacgg gcattccctt cttttcggcc
     2641 tcgaccagca gcttctgcct cacgttggac tgaaaggc
//
LOCUS       DAOPPA010000457         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_41653_length_2669_cov_3.469778, whole genome shotgun sequence.
ACCESSION   DAOPPA010000457 DAOPPA010000000
VERSION     DAOPPA010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41653_length_2669_cov_3.469778"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKH54_11965"
     CDS             complement(<1..687)
                     /locus_tag="PKH54_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD00654.1"
                     /translation="MADKMVFRFSKDFVEGDKDMKNTLGGKGANLAEMCRIGLAVPPG
                     FTITTGTCVHYFKNGNTWAPGLKEEVDAAIEWLEKVNGKKLGDTANPLLVSVRSGARA
                     SMPGMMDTILNLGLNDTTVETLANSTGNRRFAMDSYRRFIKMFGDVVLGIKGKKGEHD
                     PFESALESVKHKAGKKLDTELDAAELEEVVRLEKQVVRDRTGRDFPTDPREQLTLAIN
                     AVFDSWMNDRA"
     gene            complement(692..>2669)
                     /gene="glyS"
                     /locus_tag="PKH54_11970"
     CDS             complement(692..>2669)
                     /gene="glyS"
                     /locus_tag="PKH54_11970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOD00655.1"
                     /translation="TGDGGFTAAAAGFARGRGRTVEDLVETDSPKGRVVALRIDTPGR
                     RTIDVLAQAVPAALGGLKFPKAMRWADGRGPFVRPIHWICCLFGDSVIDFEFIGVRSG
                     RESRGHRFMSGDSFTVSSPAAYADMLRARHVIVRSQERVDLIRSQMDRVEQETGLKFI
                     RNDALFDEISNIVEKPILITCNFEEDFLRLPVEVLTTAMAAHQRYFAMQDSAGRLTRT
                     FGVVCNNQANDMAVVGRGNERVLAARLYDCRFFWENDLQTGLDSMLGRLGQRLFLKDA
                     GTMYEKMVRIRRICEDIADAVGFNEIEKADLVRGATLCKADLMSSVVCELPEVQGIMG
                     SYYAAEGGEAPEVCAAIRDHYLPRFAGDALPSADVSAALAIADRIDSIATCFHVGAIP
                     TGSRDPLALRRAAIAVLRIIISMPRMRELTVPGLLEMTRVSRQLMAGSNDFRQDVAQL
                     SRKEGAKQGKGEKAPVTVDEFMAERFRGILTDEYGVGGDVPNSVIGYLSGSDAQSPAC
                     LCGRAVAIRDALADESFRLFLDNVFKRVGNLIRKADEDFSGWRDAAASMSDPDRDYTA
                     TFFDPVEIELESARVAGMERCRSAKMNHDYAAVLKLMASFEGPLARFIGTGKDGVPVL
                     LEKDEGKRLARLALLDRIFCQFNWFADFTRISTR"
BASE COUNT          556 a          820 c          769 g          524 t
ORIGIN      
        1 ggccctgtcg ttcatccagg aatcaaagac cgcgttgatc gccagggtaa gctgttcacg
       61 gggatcggtc ggaaagtcgc ggccggtgcg atcgcggacg acctgctttt caagcctgac
      121 aacctcttcc agttcggctg cgtcaagttc ggtatcgagc ttcttgcccg ccttgtgctt
      181 gactgattcc agagcggact cgaaaggatc gtgctcgccc ttcttgccct tgataccaag
      241 gacgacgtcg ccgaacatct tgatgaagcg acgatagctg tccatcgcga aacgacggtt
      301 gccggtcgag ttggcaaggg tctcgacggt cgtatcgttc agaccaaggt tgaggatggt
      361 gtccatcatg ccgggcatgg aagcgcgggc accggatcga accgacacca gcagcggatt
      421 ggccgtgtca cccagcttct tgccgttgac cttttcaagc cactcgatgg cggcatcgac
      481 ttcttccttc aggcctggcg cccaggtatt gccgttcttg aagtaatgga cgcacgtgcc
      541 cgtcgtgatt gtgaatcccg ggggaacggc caggccgatc ctgcacattt cggccaggtt
      601 cgcgcccttg ccgcccaggg tattcttcat gtccttgtcg ccttcgacga aatcctttga
      661 gaaccggaag accatcttgt ctgccattgc gctacctcgt ggaaattctt gtgaaatcag
      721 caaaccagtt aaactggcag aagattcggt caagcagggc caggcgggcc agccgctttc
      781 cttcatcctt ttccagcaga accggaactc catccttgcc ggtgccgatg aaccgcgcca
      841 gcggtccctc gaacgaagcc atcagtttca gaaccgccgc ataatcatga ttcatcttgg
      901 cgctgcggca tctttccatg ccggccacgc gcgcggattc aagttcgatt tccaccggat
      961 caaaaaatgt cgcggtgtaa tcgcgatcgg gatcggacat cgatgcggcg gcgtcacgcc
     1021 atccagaaaa atcttcgtcg gccttgcgga tcagattgcc gacccgcttg aaaacgttat
     1081 ccaggaaaag acggaaagac tcgtcggcaa gcgcgtcgcg tatcgcgacg gcacggccgc
     1141 agaggcaagc cggcgactgg gcgtccgaac cagaaagata accgataaca gaatttggaa
     1201 cgtcgccacc cacgccgtac tcgtcggtca ggatgccacg gaaccgctcg gccatgaatt
     1261 cgtcaaccgt caccggggcc ttttcgccct ttccctgttt cgcgccttcc tttcttgata
     1321 actgcgcgac atcctgccgg aaatcgttgg acccggccat aagttgacgc gaaacgcgcg
     1381 tcatttcgag aagcccggga acggtaagtt cgcgcatgcg cggcatgctt ataatgatcc
     1441 gaagaaccgc aatggccgcg cggcgcaggg ccagcgggtc acgggatccg gtcggaatcg
     1501 cgccgacatg gaaacaggtc gcgatgctgt cgatgcggtc ggcaatcgcc agcgcggcag
     1561 aaacatcagc ggacggaagc gcgtcaccgg cgaacctggg aaggtaatga tcccggattg
     1621 ccgcgcagac ctccggggcc tcgccgcctt ccgcggcata atacgagccc atgatcccct
     1681 ggacttccgg cagttcgcac acgaccgacg acatcagatc cgccttgcag agcgtcgcgc
     1741 cccgaaccag atccgccttt tcgatttcgt tgaacccgac cgcgtcggca atgtcctcgc
     1801 agattcggcg gatccgcacc atcttttcgt acatggttcc ggcgtccttc aggaaaagac
     1861 gctggccaag ccgaccgagc atgctgtcca gaccagtctg aaggtcgttt tcccagaaga
     1921 accggcagtc ataaaggcgg gccgcgagaa cgcgttcatt tccgcgtccg acgaccgcca
     1981 tgtcgttggc ctggttgtta cagacgactc cgaaggtgcg cgtcagccga ccggcggaat
     2041 cctgcatcgc gaaatagcgc tgatgcgcgg ccatggcggt tgtcagaacc tcgaccggca
     2101 ggcgcaggaa atcctcttca aaattgcacg ttatcagtat cggcttttcg acgatgttcg
     2161 atatctcgtc gaaaagcgcg tcgttgcgaa tgaacttcag gccggtttcc tgctcgacac
     2221 gatccatctg ggaacgaatc agatcgacac gttcctggct gcggactata acgtgccggg
     2281 cgcgaagcat gtcggcatac gcggcaggcg aggaaaccgt gaacgaatcg cccgacatga
     2341 agcggtgtcc cctggattca cgccccgaac gaacaccgat gaattcgaag tcgatgactg
     2401 aatcgccgaa caggcagcaa atccagtgga ttggacgcac gaagggacca cgtccatcgg
     2461 cccagcgcat tgccttgggg aacttcaagc cgccgagtgc cgcgggaacg gcctgtgcca
     2521 gcacatcgat ggtgcggcgt ccgggggtat cgatgcgaag cgccaccacc ctgcccttgg
     2581 ggctgtcggt ttcgacaagg tcttcaaccg tgcgtcccct tccgcgggca aaaccggccg
     2641 ccgcggcagt gaatccgcca tcaccggtc
//
LOCUS       DAOPPA010000458         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_41862_length_2659_cov_3.288018, whole genome shotgun sequence.
ACCESSION   DAOPPA010000458 DAOPPA010000000
VERSION     DAOPPA010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41862_length_2659_cov_3.288018"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="PKH54_11975"
     CDS             <1..2452
                     /locus_tag="PKH54_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD00656.1"
                     /translation="KRQNQKLATGTLWGDQKAVSIETNAQLLKAEEFKPIIVSYSDGN
                     LVRLADIGEVRDDVENNRAAAWYVDERSIILMVSRQPGSNAVQVAENVKATVERLKGQ
                     LPESVQIKLLFDRSESIRESVRDVELTLLLTLVLVVLVIFLFLRNASATLIPSLALPL
                     SIIATFAVMYLLDYTLNNLTLMALTLALGFVVDDAVVMLENIYRHIEMGKSRLRAALD
                     GAREIEFTIISMTLSLVAVFIPLLLMTGIVGRLFKEFAVTISVAILASGIVSLTLSPM
                     MCSLTLKIDTRRGAHSGKPHPVWAAILGFYERTLAGVMRHRFLTVVVSVLVLAGTVIL
                     FQASPKGFLPAEDIGMISVTTEAAEGTSFDAMLKLQHQVMDVVRADDAVSGFNSSVGQ
                     YSGGLSNQGRMFINLKHRSERDVSAEEVILRLRPKVAKIPGMKVYMQVPPPIRIGGMS
                     TKSMYQFTLQSTDTKQLYEHTAKLEKELRNLPELVDVTSDLALKNPQVVVKVDRDRAS
                     ALGVSSYDVEQTLNLAYSSAWISVIYTQTNQYYVIMELLPEYQKDPDALRLLYVKTPD
                     GRLVPLGAVATQEETVGPLSVNHLGQIPAATISFDLKKGTSLNQATARIQAVADSMLP
                     DGISTSFQGQAQAFAKSESSMLILLILAILVIYMILAVLYESFVHPITILTGLPFAAF
                     GALLALYIFDMELSLYAYVGIIMLIGVVKKNAIMMIDFAIDQERNGASSRDAILKAAS
                     VRFRPIMMTTVAALMGTLPIALGLGAGGESRQPLGLAVVGGLLFSQLITLYVTPVFYT
                     YFDRLQTASARIFGHHEA"
BASE COUNT          533 a          800 c          762 g          564 t
ORIGIN      
        1 gaaaaggcag aaccagaagc ttgcgaccgg aaccttgtgg ggcgaccaga aggccgtttc
       61 aatcgagacg aacgcgcagc tcctgaaggc ggaagaattc aagcccatca tcgtcagcta
      121 ttccgatggt aacctggttc gccttgcaga catcggtgaa gtccgggacg acgtcgagaa
      181 caaccgtgcc gcggcctggt acgttgacga gcgctccatc atcctgatgg tttcgcgaca
      241 gccgggaagc aatgcggttc aggtcgcgga aaacgtgaag gcaaccgtcg agaggttgaa
      301 gggacagctt ccggaatcgg ttcagatcaa gctgctgttc gatcgatccg aatcaatcag
      361 ggaatcggtg cgcgatgtcg agctgacgct gctgctgacc ctggttctgg tggttcttgt
      421 catcttcctt ttccttcgga acgcgtcggc cacgctgatt cccagccttg ccctgcccct
      481 ttccatcatc gccacgttcg cggtgatgta ccttctcgac tatacgttga acaacctgac
      541 actgatggcg ctgaccctgg cgctcgggtt cgtcgtcgat gacgccgtgg tcatgctgga
      601 aaacatctat cgccacatcg agatgggaaa atccaggctg cgggccgcgc ttgacggcgc
      661 acgggagatc gagttcacca tcatctcgat gacgctttcg cttgtggcgg ttttcatccc
      721 gctgctgctg atgaccggca tcgtcggccg ccttttcaag gaattcgcgg tgacaatcag
      781 cgttgccatc ctggcgtccg gcatcgtttc actgaccctt tcgccgatga tgtgcagcct
      841 gacgctgaaa atcgacacgc gcaggggcgc ccattcgggc aagccgcatc cggtctgggc
      901 cgcgatcctc ggattttatg aacggacgct tgccggcgtc atgcgccacc gtttcctgac
      961 cgttgtcgtg tcggttcttg tgctggcagg aacggtgatc ctgttccagg caagcccaaa
     1021 gggcttcctg cccgccgagg atatcgggat gatctcggtc acgaccgagg cggccgaagg
     1081 cacgtctttc gacgcgatgc tgaagttgca gcaccaggtc atggacgtcg tccgggccga
     1141 cgatgccgtc agcggattca acagcagcgt cggccagtac tcgggcgggc tttcgaacca
     1201 gggtcgcatg ttcatcaacc tgaagcaccg atccgaacgc gacgtttccg ccgaggaggt
     1261 catcttacgc ctgcgtccga aggtcgcgaa gattcccgga atgaaggtct atatgcaggt
     1321 tccgccgccg atacgcattg gcggaatgtc aacgaagtcg atgtaccagt tcaccctgca
     1381 gtcaaccgac acgaagcaac tttacgaaca cacggcgaag cttgaaaagg aactgcgcaa
     1441 ccttcccgaa ctggttgacg tgaccagcga cctggccctg aaaaacccgc aggtcgttgt
     1501 aaaagttgac cgtgaccgcg cctcggccct tggcgtctcg tcgtacgacg tcgagcagac
     1561 cttgaacctg gcatacagtt cggcctggat ttcggtgatt tatacccaga cgaaccagta
     1621 ttacgtgatc atggagctgc ttccggaata ccagaaggat cctgacgcgt tgcggctgct
     1681 gtacgtcaag acgcccgacg gcaggctggt tccgcttggc gcggtcgcga cacaggagga
     1741 aacggtcggc cccctttcgg tcaaccacct gggacagatc ccggccgcca cgatatcgtt
     1801 cgatctgaaa aagggcacgt cactcaacca ggcaacggcc aggatccagg ccgttgcgga
     1861 ctcgatgctg cccgacggca tctcgacatc cttccaggga caggcccagg cattcgcgaa
     1921 gtcggaatca tcgatgctga tcctgctgat cctggcgatc ctggtgatct acatgatcct
     1981 ggccgtcctg tatgaaagct tcgtccatcc gataaccatc ctgaccgggc ttcccttcgc
     2041 ggccttcggc gcgctgcttg ccctttacat cttcgacatg gaattgagcc tgtacgccta
     2101 tgtcggaatc atcatgctca tcggggtcgt caagaaaaat gccatcatga tgatcgactt
     2161 cgcaattgac caggaaagga acggcgcatc gtcgagggac gcgatactga aggcggcgtc
     2221 cgtccgcttc cgcccgatca tgatgaccac cgtcgccgcc ctgatgggaa ctctgccgat
     2281 cgccctggga cttggcgccg gtggcgaatc gcgccagcct cttggcctgg cggtcgtggg
     2341 cggcctgctg ttttcacagt tgatcaccct ttacgtcaca ccggtcttct acacgtactt
     2401 cgaccggctg cagacggcgt cggcccgcat cttcggccac cacgaggcct gacttgccga
     2461 ccattggaag tctgagcgac tcgacgaccc tcacgggcgg gctgttcgca ccgaaagggc
     2521 aacaatctat ttgccggcga acatcttgct gaagaatcca ggggtttcaa tgccgagcga
     2581 gcggtcgatc atgcccttga gttcttcctt cggggcatag cctatcttgt ggtcaaacac
     2641 ttcctttccc ttgaaaagg
//
LOCUS       DAOPPA010000459         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_41964_length_2653_cov_3.472671, whole genome shotgun sequence.
ACCESSION   DAOPPA010000459 DAOPPA010000000
VERSION     DAOPPA010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41964_length_2653_cov_3.472671"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="PKH54_11980"
     CDS             complement(<1..1733)
                     /locus_tag="PKH54_11980"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD00657.1"
                     /translation="MKIFEPGQIRNVGLYGHQGSGKTTLAEAMVYLGKTSNRLCSVQE
                     GNSNFDFDSEEIRKRSSISAAVGYAQWNKKLINIVDVPGDTNFAAESVMAVMGTDLAL
                     IVVSAVDGVQVGTEKAIELLLENEIPMMVVVTKVDRERADYGKIVADLQNRYGNAVVP
                     MALPTGNESNFKGIVDLVNNCAFTYADPMNGAKGDVPAAMASAVSDAREPLVEKLAEQ
                     DDDLMEKYFGEGELSTEDLRTALTVGFKKAGVIPVLPVNAATAMGVDFLMNVIAGHGP
                     SPLDRKGLKVQKGDATTDLVIDPDGKFMAYVFKSIVDLQAGRISVFRVASGSIAQDGQ
                     FVNLSTGAKERFGSLVKLLGKKQEQVEKAVCGDIVAVVKLKETNSGHTIGAEDGMGTL
                     VLPPLPTPCISFAVKPKNTSDEDKLSAAIQKIVEEDPGLRLSRDDESKEFLVSGLGQV
                     HVQSAVDKLKNKFNVDVELKLPRIPYRETIRGAAKNVEGKHKKQSGGRGQFGVCYIDM
                     GPNVRGSGFEFEDAIFGGSIPKQFIPPIKKGMEDCMTRGVVAGYPVVDIKVKLIDGKY
                     HDVDSDARSFE"
     gene            1972..2409
                     /locus_tag="PKH54_11985"
     CDS             1972..2409
                     /locus_tag="PKH54_11985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD00658.1"
                     /translation="MPEVTNVRVEIVDGIPVADHIALLREAGWFRDGEDPSAMRRLLD
                     GSFLIAAAIDSQGRTIGMGRMISDGASDGYIQDVVVQASSRGLGLGRRIVSLLAAEGR
                     RRGLGWIGLVAAPGTVPFYRALGFEPMPDHVPMLLKIPDGNPR"
     gene            2416..>2653
                     /locus_tag="PKH54_11990"
     CDS             2416..>2653
                     /locus_tag="PKH54_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00659.1"
                     /translation="MNLAFMGHGAASPSTRIEFHDFKLDDRLFLEKFFKAGGGRLCEY
                     SFAVQYCWQSYNKSVWAILNDTLVIRFETGGQFRF"
BASE COUNT          524 a          758 c          770 g          601 t
ORIGIN      
        1 atttcgaacg aacgggcgtc ggaatcgacg tcatgatact ttccgtcgat cagcttgacc
       61 ttgatatcga cgacggggta accggcgaca acgccacggg tcatgcaatc ttccatgccc
      121 ttcttgatcg gcggaatgaa ctgcttcgga atcgacccgc cgaaaattgc atcctcgaac
      181 tcgaatccgc ttccgcggac gttcggtccc atgtcgatat agcagacgcc gaactggcca
      241 cgaccgccgg actgcttctt atgctttcct tcgacgttct tcgcggcgcc gcgaatcgtt
      301 tcgcggtacg gaatcctggg cagcttcagt tcgacatcga cgttgaactt gttcttcagc
      361 ttatcgactg ccgactgaac gtgaacctgg ccaagtccgc tgacaaggaa ttccttcgat
      421 tcgtcgtcgc gggacaggcg cagccccggg tcttcctcga cgatcttctg gattgctgcc
      481 gaaagcttgt cttcatcgct ggtgttcttc ggcttgactg cgaacgaaat gcacggggtc
      541 ggcagcggcg gcagaaccag cgtacccatg ccgtcctcgg caccgatcgt gtggccggaa
      601 ttggtttcct tcagcttcac gacggcgacg atgtcgccgc ataccgcctt ttcaacctgt
      661 tcctgcttct ttccaagcag cttgacaagg ctgccgaagc gctccttggc gccggtggaa
      721 agattcacga actggccatc ctgggcgatg gaaccggatg cgacgcggaa aacgctgatg
      781 cggccggcct gcaaatcgac gatgctcttg aagacgtaag ccatgaactt gccgtcggga
      841 tcgatgacaa gatcggtcgt ggcatcgccc ttctgaacct tcaggccctt gcggtcgagc
      901 ggcgacgggc cgtgacctgc aatgacgttc atcaggaaat cgacgcccat ggccgttgcc
      961 gcgttgactg gcagaacggg gatgacgccg gccttcttga acccgaccgt cagtgcggtg
     1021 cgaagatctt cggtcgaaag ctcgccttcg ccgaaatact tttccatcag gtcgtcgtcc
     1081 tgttcggcaa gcttttcaac caggggttcc ctggcgtcgg aaacggcgga agccattgcc
     1141 gcggggacgt cgcccttggc gccgttcatc ggatcggcat acgtgaaggc gcaattgttg
     1201 accaggtcaa cgatgccctt gaaattggat tcattgccgg taggaagggc catcggaacg
     1261 acggcgttgc cataacggtt ctgaaggtcg gccacgatct tgccgtagtc ggcgcgctcg
     1321 cgatcgacct ttgtcaccac gaccatcatc ggaatttcgt tttcaagaag cagttcgatg
     1381 gccttttcgg ttccaacctg cacgccgtcc acggcgctga ccacgataag cgcaaggtcg
     1441 gtgcccatca cggccatgac cgattcggcg gcgaaattgg tgtcgccggg gacatcgacg
     1501 atgttaataa gcttcttgtt ccactgtgcg tatccgacag cggcggaaat cgagctgcgc
     1561 ttgcgaattt cttcggaatc aaagtcgaag ttcgaattgc cttcctggac gctgcaaagc
     1621 ctgttggaag tctttcccag atagaccatc gcttcagcca gcgtagtctt gccagagccc
     1681 tgatgtccat acagaccgac gttgcggatt tgtccaggtt caaagatctt catatttcaa
     1741 taccttcaga aagacgccaa aaccaggtcc ggcgacaccg ggccacccag cgcaaacttt
     1801 gcgggcgctg ttttgtattc gattccggtc gcagtgtcaa tccaaagccg cacgaaagga
     1861 ttgattatca ggcgcgaaaa tcggtgcgga attgcctgcg atcgtcactt gacggcgccg
     1921 ggtgctgttc ttattgttcc cgtggccaaa gaatccggat taaccgtgga aatgcccgaa
     1981 gtcacaaacg tcagggtcga aatcgttgac ggaattcccg tggcggatca cattgccctg
     2041 ctgcgcgagg ccggctggtt ccgggacggg gaagatccgt cagccatgcg acgccttctt
     2101 gatggctcct tcctgatcgc cgcggccatc gacagtcagg ggcgtaccat tggaatggga
     2161 cggatgattt cggacggggc cagcgacggg tatatccagg acgttgtcgt ccaggcttca
     2221 tcaagagggc tgggccttgg gcgaaggatt gtcagtctgc tggccgccga gggccgacgc
     2281 aggggcctcg ggtggatcgg cctggtcgcc gcgccgggaa ccgttccatt ttaccgggcg
     2341 cttggcttcg agccgatgcc ggatcatgtg ccgatgctgt tgaagattcc ggacgggaat
     2401 ccacggtgac catttatgaa ccttgccttc atggggcatg gggccgcgag cccatcgacc
     2461 cgaatcgaat ttcacgattt caaacttgat gaccgccttt tccttgaaaa gtttttcaag
     2521 gctggcggtg ggcgcctttg cgagtattcg ttcgccgtcc agtattgctg gcagtcctac
     2581 aacaagagcg tgtgggcgat tcttaacgac acgctggtca tcaggttcga gaccggcggt
     2641 cagtttcgat tca
//
LOCUS       DAOPPA010000460         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42172_length_2643_cov_3.323802, whole genome shotgun sequence.
ACCESSION   DAOPPA010000460 DAOPPA010000000
VERSION     DAOPPA010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42172_length_2643_cov_3.323802"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PKH54_11995"
     CDS             complement(<1..1693)
                     /locus_tag="PKH54_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD00660.1"
                     /translation="MRKSKWLAILAAAAMIAAIPGCGSDEGVFDVLDSSTADVQYPDT
                     VDPDIDQPFDANRPDLNGADLNGIDLAGYDLNGVDLGDFELPDKDTNVDQDGTDIGDP
                     DGTEPDGVDPDAIDPDGQNPDVNDPDATDPDAVDPDANHPDVVNPDNGGGDTGGDTTV
                     EPTECGNPPIPDVASGNCSFTEGSNYLLLRGDVFGRENILKNAMVLIDPTGFITCAAC
                     DCTGATGYAGATVIACPDALITPGLINGHDHVTFGGNAPKPHTVKYDHRHEWRTGKVP
                     GKPKISVPSTSGAEPWTELRHIAGGATSMFGSGSAQGLLRNLDRELMDIPSTYQAKYD
                     TFPLDDTDGTMLTSGCQYPGITTWSSVSSFKSYVPHISEGINLAANNEFKCLSDGSGG
                     GQDLVRENSGIIHGIGLTAGDIAQMAIDGTGLIWSVRTNIDLYGNTASITLFDKLGVD
                     IGIGPDWTASGSMNMMRELNCVDYLNRNHFNGHFSDWRIVEMATAGNARVFNLLDKIG
                     QLKPGLLADIAIFSTASHPAERAIIDGNPGDVIMTMRSGIPLYGDANLVDGLLPGD"
     gene            1925..>2643
                     /locus_tag="PKH54_12000"
     CDS             1925..>2643
                     /locus_tag="PKH54_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOD00661.1"
                     /translation="MSVLFPRIPVTPLKTIFLFLFVLLVLLPTTALAFGPVAHVDMAL
                     EILSWAGMVGGSLGGLIARYRRHFILGTLDPDRTLAKNLATYEDHSHNWDTAIDQFRS
                     AQSDQERARSLGRICHLAADVVAHNYFVPKKIVDSVESRGAGHFFWEVRFDARLREIR
                     GQDELESLSFNDREEIRYLSTMIRPTVATKNLNARLTGVAMKLQKAGPYMAATRTLDR
                     RSNLDFTDAEVMDIREMAMQA"
BASE COUNT          502 a          794 c          762 g          585 t
ORIGIN      
        1 cgtcacccgg cagcagcccg tcaacaaggt tggcatcccc gtaaagcgga atgccgctgc
       61 gcatcgtcat gattacatcg cccggattcc cgtcgatgat ggcacgctcg gcagggtggc
      121 tggcggtcga aaatatcgcg atgtccgcca gcaggccggg cttcaactgt ccaatcttgt
      181 ccagcaggtt gaagacgcgc gcgttcccgg cggtcgccat ctcgacgatg cgccagtcgc
      241 tgaagtgacc attgaagtgg ttccggttca ggtaatcgac gcagttcagt tcgcgcatca
      301 tgttcatgga accggaagcg gtccagtccg gcccgatgcc gatatcgacg ccgagcttgt
      361 caaacagcgt aatcgacgcc gtgtttccgt aaaggtcgat gttggtgcgc accgaccata
      421 taaggccggt tccatcgatc gccatctgcg cgatgtcgcc ggcggtcagg ccgattccgt
      481 ggatgatgcc gctgttttcg cgaaccaggt cctggccgcc gccggaacca tctgacaggc
      541 acttaaattc gttgttcgcc gcaaggttga tgccttcgga aatgtgcgga acgtatgact
      601 tgaacgatga aaccgaactc caggtcgtga tgccggggta ctggcatccc gaagtaagca
      661 ttgttccatc ggtatcgtcc agcgggaacg tgtcgtactt tgcctggtac gtgcttggga
      721 tatccatcag ttccctgtcc aggttccgca gcagtccctg tgccgagccg gaaccgaaca
      781 tcgaggtcgc gccgccggca atgtgccgca attcggtcca cggttccgcg ccgctggtgc
      841 ttggaacgga aatcttcggc ttgcccggta cctttccggt ccgccattcg tgacggtggt
      901 cgtatttgac ggtatgcggc ttgggcgcgt tgccgccaaa ggtcacgtgg tcgtggccgt
      961 tgatgagccc gggggttatc agggcatccg ggcaggcgat gaccgtcgcg ccggcataac
     1021 cggtggcgcc ggtgcagtcg caggcggcgc aggtaatgaa accggtcggg tcaatcagca
     1081 ccatcgcgtt cttcaggatg ttttcccgac caaagacgtc gccgcgaaga agcaggtagt
     1141 tcgaaccctc ggtgaacgaa cagttgccgg acgcgacgtc cggaatcggc gggtttccgc
     1201 attcggtcgg ctcgacggtc gtgtccccgc cggtgtcgcc accgccgtta tcgggattga
     1261 ccacgtccgg atggttggcg tcgggatcaa cggcatccgg gtcggtcgcg tcgggatcat
     1321 taacatccgg attctggccg tcggggtcga tggcgtccgg atcgactccg tcaggttccg
     1381 tgccatcggg atccccgata tcggtgccat cctggtcaac attcgtatcc ttgtcgggaa
     1441 gctcgaaatc cccaaggtca accccgttaa ggtcataccc cgcaaggtcg atgccgttca
     1501 gatcggcccc gttaagatcc ggcctgttcg catcgaatgg ctggtcaata tcgggatcaa
     1561 cggtgtccgg gtactgaaca tccgccgtac tcgagtcaag tacgtcaaac accccttcgt
     1621 ccgaaccgca tcccgggatt gcggcaatca tcgccgccgc cgcaagaata gccagccact
     1681 tgctctttct catgtcatgc ccccaataat catggcgtcc ttcaaaaccc ggcgtccgga
     1741 aggacgcgga ttaccgacaa ggttatccct ttttccacgc aaccggaaca catcccgccc
     1801 aaatccttga ttttatggtc gggaaccacg catgccgcca gaaatatgaa gaaaattgcg
     1861 attctttctt gatcctgaat tttcatcccg tagaattgca ttcgcgaagt ccaaggcgga
     1921 tcagttgtcc gttcttttcc cgcgaattcc ggtgacgcct ttgaagacca tcttcctttt
     1981 tctgtttgtc ctgcttgtct tgctgccgac gaccgcgctt gccttcgggc cggtcgcgca
     2041 cgtcgatatg gcgcttgaaa tcctgtcgtg ggcgggtatg gtcggcgggt cgcttggcgg
     2101 cctgattgcc cgctaccgtc gccatttcat ccttggcacg ctggatccgg atcgcactct
     2161 tgcaaagaat cttgcgacct atgaagacca ttcccacaac tgggacaccg ccatcgacca
     2221 gttccggtcc gcccagtcgg atcaagaacg ggcccgctcg cttgggcgca tctgtcacct
     2281 ggcggccgat gtcgtggcgc acaattactt cgttcccaag aagattgtcg attccgtcga
     2341 gtcaaggggt gccggccact tcttctggga agttcgcttt gatgccaggc ttcgcgaaat
     2401 ccgcgggcag gatgaactgg aaagtctttc cttcaatgac agggaggaaa tccgttacct
     2461 ttcgacgatg attcgcccaa cggtcgcgac gaagaatctt aacgcccgtc ttaccggcgt
     2521 cgccatgaag cttcagaagg ccggccctta catggcagcg acgcgcacgc ttgaccgccg
     2581 ttcgaacctg gatttcaccg acgccgaagt aatggatatc cgtgagatgg cgatgcaggc
     2641 cca
//
LOCUS       DAOPPA010000461         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42491_length_2629_cov_4.203574, whole genome shotgun sequence.
ACCESSION   DAOPPA010000461 DAOPPA010000000
VERSION     DAOPPA010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42491_length_2629_cov_4.203574"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKH54_12005"
     CDS             complement(<1..322)
                     /locus_tag="PKH54_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD00662.1"
                     /translation="MESSKHLYALLLAGGKGKRFWPLSREENPKQLIQLFSSQTLIEE
                     TYERIRPLIPQERVLLATSQALMMRFKGLFSGLDARNFIVEPLPRNTAPCIAVAARKA
                     VARDP"
     gene            complement(405..1436)
                     /gene="amrS"
                     /locus_tag="PKH54_12010"
     CDS             complement(405..1436)
                     /gene="amrS"
                     /locus_tag="PKH54_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD00663.1"
                     /translation="MNIDGAGQAAFFTSEANGNVRCHLCPHGCVIARGSAGKCNVRMN
                     HDGVLYLTVWGRPAALQLNAIESMPLYHFLPGTETFCIGTAGCTMDCAFCNTSEIVSL
                     LPNDLPFIPLDPEAVVTRARDNGCASISFAFNEPVACAEYMMAVSREAARTELKCVAF
                     TNAFVSRPVIRDVFRDIHAANVGIKAFEVTMYRDFCKGRLDDVLRSTLELAELGLHLE
                     LTMAVIPGLNDSTRAMQAMAAWIRDSLGMDTPLHLVPFQPSHRLTTIRKPDTQSIDKL
                     AAAARNEGLRHVYGGTPDSAFCDTKCHKCGRVMIHRNGSTFMSIDVPASRKCPDCGTP
                     LLMVDRRPS"
     gene            1503..2282
                     /gene="map"
                     /locus_tag="PKH54_12015"
     CDS             1503..2282
                     /gene="map"
                     /locus_tag="PKH54_12015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOD00664.1"
                     /translation="MILIKSDHEIALMRDAGKILAEVLMGMNERIVPGVTTQELDDWA
                     DSRIAAAGCVATFRGQPGMVRGARPFPAATCISINEEIIHGIPSKQRVIKDGDIVSVD
                     CGVTYKGYVADSAFSRIAGSGSPDLVRLLETTKRSLYAGIEKARPGGRLGDISFAVES
                     WAVAEGLGIVRDFCGHGVGRSLHEDPGIPNYGKPGTGPLLRQGMTLAIEPMFMLGKEK
                     VKVLVDGWTVVTSDRSPAAHWEHTILITDNGPEILTKWAQV"
     gene            2390..>2629
                     /locus_tag="PKH54_12020"
     CDS             2390..>2629
                     /locus_tag="PKH54_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00665.1"
                     /translation="MLKTHLALMDELKDYASPRAKLTRMLKSGSIVQLRRGLFVDDVS
                     ISPRVVAPVLYGPSYISFEYVLATAGLIPERVAAVT"
BASE COUNT          531 a          752 c          829 g          517 t
ORIGIN      
        1 ccgggtcgcg cgcgacggcc ttcctcgcgg ccacggcgat gcagggggcc gtgttgcggg
       61 gaagcggttc gacgatgaag ttcctggcgt caaggccgct gaaaaggccc ttgaatcgca
      121 tcatcagggc ctgggacgtg gccagcagaa ccctttcctg cggaatcaga ggacggatgc
      181 gttcatacgt ttcctcgatc aatgtctggg aagaaaacaa ctggataagc tgcttcgggt
      241 tttcttcgcg agacagcggc cagaagcgct tgcccttgcc gccggccaga agaagcgcgt
      301 aaagatgctt cgaagattcc atggtgatcc cccgaaccgt caagcgtgtc gaaccgttca
      361 acgacaacgg ttcagactgc cacaaacggg cggcaaatgc aatttcagga agggcgtcga
      421 tcgaccatca gaagtggcgt tccgcagtcg gggcattttc gcgatgctgg cacgtcaatc
      481 gacatgaaag ttgacccatt ccggtgaatc atcacgcgtc cgcatttgtg gcatttcgtg
      541 tcgcagaatg ccgaatccgg agttccccca tagacgtggc gcaatccctc gttgcgggcg
      601 gccgcggcaa gcttgtcgat tgactgcgtg tccggcttcc ggatggtcgt aagccggtgt
      661 gacggctgga aaggaaccag gtgaagcggc gtgtccatgc caagggaatc gcggatccat
      721 gccgccattg cctgcatcgc gcgggtcgag tcgttcaggc cggggatgac agccatcgta
      781 agttccaggt gaagccccaa ttcggccagt tccagtgtcg aacgcagcac gtcatcaagg
      841 cgccccttgc agaagtcgcg atacatcgtc acttcgaagg ccttgatgcc cacgttcgcg
      901 gcgtgtatgt cgcggaagac gtcgcgaatg acgggcctgc tgacaaatgc gttcgtgaac
      961 gcgacgcact tcagttcggt tcttgcggcc tccctggaaa cggccatcat gtattcggcg
     1021 caggcaacgg gctcgttgaa cgcaaatgat atcgacgcac atccgttgtc ccgggcgcgg
     1081 gtgacgacgg cctcgggatc aagtggaatg aacggcaggt cgttgggcaa caggctgacg
     1141 atttcgcttg tgttgcagaa tgcgcagtcc atcgtgcatc ccgcggtccc gatgcagaac
     1201 gtctcggtgc caggcaggaa gtggtaaagg ggcatgcttt cgatcgcatt gagctgcaac
     1261 gcggccgggc gtccccatac cgtaaggtac agcacgccat cgtggttcat gcgcacattg
     1321 catttgccgg ccgatcccct ggcgatgacg caaccgtgcg gacacaggtg gcaacgcacg
     1381 tttccgttgg cttccgacgt gaagaaagcc gcctgtccgg caccgtcgat gttcatccga
     1441 atccttgcga aatcgtccgt ccgggtgcat tgtacgcctg aaagcgagtg aggacacaga
     1501 aggtgatcct gatcaagtcc gaccatgaaa tcgccctgat gagggacgcg ggaaagatac
     1561 ttgccgaagt gctgatggga atgaacgaac gcatcgttcc gggtgtgacg acgcaggaac
     1621 ttgatgactg ggccgacagc cggattgcgg cggcaggctg cgtggcgacc tttcgcggac
     1681 agcccgggat ggtgcgcggg gcccggccgt ttccggcggc gacgtgcata tcgatcaacg
     1741 aggaaatcat ccacggcatc ccgtctaagc agcgggtgat taaggacggc gacatcgtca
     1801 gcgtcgattg cggcgtgacg tacaagggct acgtcgcgga ttcggctttt tccagaatcg
     1861 ccggcagcgg cagccccgat ctggtgcgcc ttctggaaac gacgaagcgt tcactgtacg
     1921 ccggcatcga aaaggcccgt cccggcgggc gcctgggcga tatcagcttc gcggtcgagt
     1981 cgtgggccgt tgcggaagga ttgggcatcg tgcgcgactt ctgcggccac ggcgtcggcc
     2041 gcagcctgca cgaagacccc ggcatcccga attacggcaa gccaggcacg ggcccgctgc
     2101 tgcgccaggg catgacgctg gcgattgaac cgatgttcat gctgggcaag gaaaaggtca
     2161 aagtgcttgt cgatggctgg acggtcgtca cgtcggaccg cagccccgcc gcccactggg
     2221 aacacaccat cctgataacc gacaacggcc cggaaatcct tacgaagtgg gcccaggtct
     2281 gacgcttttc caagcaatgc cgccaggaac cttgcagcac ggctctgtcg aacttgttgc
     2341 ttgattgtct ccagaaagta tcgtaaattg atacttgccg gaggtaatga tgcttaagac
     2401 ccacctggcg ctgatggatg aactgaagga ttacgcgtcg cccagggcaa agctgacgcg
     2461 gatgctgaag tccggttcaa tcgtgcaatt gcggcgcggg ttgttcgtcg atgacgtctc
     2521 gatttcgccc cgcgtggttg ccccggttct gtacggtccg tcatacattt cctttgaata
     2581 cgtgctcgcc accgccggct tgattccgga acgggttgcc gcagtcact
//
LOCUS       DAOPPA010000462         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42535_length_2627_cov_3.528771, whole genome shotgun sequence.
ACCESSION   DAOPPA010000462 DAOPPA010000000
VERSION     DAOPPA010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42535_length_2627_cov_3.528771"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="PKH54_12025"
     CDS             complement(<1..2019)
                     /locus_tag="PKH54_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOD00666.1"
                     /translation="MSFRIRKAAVLGGGVMGSGIAAHLANAGIKTYLLDMVPPGVGPD
                     ASHDERSAFALKGIENALKFKPAPLFYDKGGAELVFAGNFDDDIEKIAECDIVVEAIV
                     ENLEIKRQLFAKVVPHMKQDAILSSNTSGLSIASMAEALPFFMRDRFLVTHFFNPVRF
                     MKLLELVPGPDTSAETLQIWADFGRKKLGKGVVFGKDTPNFVGNRIGIYAIAKTVEAM
                     MADGFSVEEIDSIFGKPMGRPKTAAFKTLDLVGLDVYAHVSTTCLEKLENDPEKGSFK
                     VPDFINAMIAKGLLGAKKKAGFYTKGPNGERMVIDPATLEYKPIEKPRLELIGIVKGM
                     ALNDRVKTMVKSDDRVGKLAWKITAQTLIYAANRVGEIADDVVNIDNAMKWGFGWEQG
                     PFETWDAIGVEESVARMQAEGYQVPETVLKMLASGAKSFYGRQGVDKTFFDFATGQYK
                     VLETPPWQLTIKGFKDQGKVIKKNMGASLIDIGDGIACLEFHTKMNALDEDIMSMILA
                     SCDIVEDRFEGLVVTNDGDHFSAGANIAMILMAAKTGNWELLEQSIRVLQSCGSRLKY
                     CAKPTVVAPFGMALGGGCEVSIQGAALRPHAELYMGLVEVGVGVIPAGGGCKEMTCRA
                     LSHIPGDNADLSRLPYIAKVFENIATAKVSTSAFDARRYGYVRKYDQIT"
     gene            complement(2194..>2627)
                     /locus_tag="PKH54_12030"
     CDS             complement(2194..>2627)
                     /locus_tag="PKH54_12030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707703.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOD00667.1"
                     /translation="ITMAKQGSAIRGLMDSFATSVSIALQYGVPLEVLVSKFAHVRFE
                     PNGFSSNPAIGYAKSIVDYVFRWLELKFISNTAWQRRTEVPLDLEAMAGDDAPPPEVE
                     KQNDLFQGQEDAPPCSNCGWIMVRNGKCYKCVNCGSTSGCS"
BASE COUNT          545 a          790 c          746 g          546 t
ORIGIN      
        1 ggttatctgg tcgtacttgc ggacgtaccc gtatctcctg gcgtcgaaag cacttgtcga
       61 gaccttcgcg gtcgcgatgt tctcgaacac cttggcgatg taaggaagtc tggagagatc
      121 cgcgttgtcg ccaggaatgt gggacagggc gcggcaggtc atttccttgc agccgccgcc
      181 ggcgggaatc acgccgacgc cgacttcgac cagacccatg tagagttcgg cgtgcggacg
      241 cagggcggcg ccctggatgg aaacttcgca gccgccgcca agcgccatcc cgaacggggc
      301 gacaactgtc ggcttcgcgc agtacttcag gcgggaacca cagctctgca ggacgcggat
      361 gctctgttcc agaagttccc agttcccggt cttcgccgcc atcaggatca tcgcgatgtt
      421 ggcgccggcg ctgaagtgat cgccgtcgtt tgtcacgacc agaccttcga accgatcctc
      481 gacgatgtcg caggacgcaa gaatcatcga catgatgtcc tcgtccagag cgttcatctt
      541 cgtgtggaat tcaaggcagg cgatgccgtc gccgatatca atcaggctgg cacccatgtt
      601 cttcttgatg accttgccct gatccttgaa tcccttgatg gtcaactgcc acggcggtgt
      661 ctcgagaacc ttgtactgtc cggtcgcgaa gtcgaagaac gtcttatcga cgccctggcg
      721 tccatagaac gacttcgcac ccgaagccag catcttcagc acggtctcgg gaacctggta
      781 gccttcagcc tgcatcctcg cgacgctttc ctcgacgcca atcgcgtccc acgtctcgaa
      841 cgggccctgt tcccagccga agccccactt catcgcgttg tcgatgttga cgacgtcatc
      901 cgcgatctcg ccgacacgat tggccgcgta aatcaaggtc tgcgccgtga tcttccacgc
      961 aagcttcccg acccggtcgt cggacttgac catcgtcttg acgcgatcgt tcaaggccat
     1021 gcccttcacg atgccgataa gctcaagccg cggcttctcg atcggcttgt attcaagcgt
     1081 cgcgggatcg atgaccatgc gttcgccgtt cggccccttg gtatagaagc ccgccttctt
     1141 cttggcaccc agaaggccct tggcaatcat ggcattgatg aaatccggaa ccttgaaaga
     1201 gcccttttcg ggatcgtttt caagcttctc gaggcaagtt gtcgaaacat gcgcgtagac
     1261 atcaaggccg acaaggtcga gggtcttgaa cgcggccgtc ttcgggcgtc ccatcggctt
     1321 gccgaagatg gagtcgattt cctcgaccga gaagccgtcc gccatcatgg cttcaacggt
     1381 cttggcgatc gcgtagatgc cgatgcggtt accgacgaaa ttgggggtgt cctttccaaa
     1441 aacaacgccc tttcccagct tcttgcgacc gaaatcggcc cagatctgaa gcgtctcggc
     1501 gctggtgtcc ggtccgggaa ccagttccag aagcttcatg aaacgaaccg gattgaagaa
     1561 gtgcgtcacc aggaagcggt cgcgcatgaa aaacggaagt gcctcggcca tcgaggcgat
     1621 cgaaagaccg gaggtgttcg acgaaaggat cgcgtcctgc ttcatgtgcg gaacgacctt
     1681 cgcgaaaagc tgccgcttga tttccaggtt ttcgacgatc gcctcgacaa cgatatcgca
     1741 ctcggcgatc ttctcaatgt cgtcgtcgaa gttgcccgca aaaacaagtt ccgccccgcc
     1801 cttgtcgtag aacagcggcg ctggcttgaa cttcagggca ttctcgattc ccttcagggc
     1861 gaaagccgaa cgctcgtcgt gcgacgcgtc cgggccgacg ccaggtggaa ccatgtcaag
     1921 cagatacgtc ttgattccgg cgttggccag gtgggctgcg atgcccgaac ccattacgcc
     1981 accgccaagg acagcggcct tgcggatgcg aaaagacata ggactcctcc acattcaggt
     2041 ttgtgaacag aaccggctat aaaggcacaa cctgtcaatg aaatcacgca ggaagaagga
     2101 aatcaagcac gacaacggtt gttaccagaa tgacaggatt tttatccaga aacagcgtcc
     2161 tggaaagggg ggtgtggaag gacgggaagc cgcctatgaa cagccggaag tcgagccgca
     2221 gttgacgcac ttgtagcact ttccgttgcg caccattatc cagccgcaat tggagcaggg
     2281 gggcgcgtcc tcctggccct ggaacaggtc gttctgcttt tcgacctcgg gaggcggcgc
     2341 gtcgtccccg gccatcgcct ccagatcaag cggaacctcg gttcttctct gccatgcggt
     2401 gttgctgatg aatttcagtt ccagccagcg gaagacgtag tccacgattg atttcgcgta
     2461 accgattgcc ggattggagc tgaagccgtt cggctcgaac cttacgtgag caaacttcga
     2521 caccaggact tccaggggca cgccgtactg caacgcgatg gaaaccgacg tcgcgaagct
     2581 gtccatcagg ccgcggatcg ccgacccctg cttggccatc gtgatga
//
LOCUS       DAOPPA010000463         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42540_length_2627_cov_2.840980, whole genome shotgun sequence.
ACCESSION   DAOPPA010000463 DAOPPA010000000
VERSION     DAOPPA010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42540_length_2627_cov_2.840980"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKH54_12035"
     CDS             complement(<1..611)
                     /locus_tag="PKH54_12035"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOD00668.1"
                     /translation="MTREDEIAGYRPTVEVRVVVCGHGRVGGSFARSCVLAAIPTWVI
                     DVQSVVYQMPTILPIGMNAAMSVKFVVPSSVPTFWYLCTPEHVNDDLVRQICESPGFN
                     RATDCIAVASATWDTSRVPEDLGASVGRAHPLHPFPPAATLMPVPCGTPFGTSGLPEF
                     AIHLLESMGFVPFALDDQDRYLYHAAAVLVSNLPAALVQQAAG"
     gene            complement(686..1237)
                     /locus_tag="PKH54_12040"
     CDS             complement(686..1237)
                     /locus_tag="PKH54_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HOD00669.1"
                     /translation="MRGFTAVFGGSFDPPHLGHSQAVSWLLARGGCGRVIVIPAFSHA
                     FGKEMADFEHRLQMCRIAFFQFGAAVEVSDIEAMLPTPSFTVNTVTTLHAMYPTENLR
                     LVIGSDILAETDDWKDFDRITAIAPPIVLNRGGFTGGGLGSFPAIASSGVRGRIAAGD
                     RCDDAVDGDVMNYIEKFGLYGLT"
     gene            complement(1282..2475)
                     /locus_tag="PKH54_12045"
     CDS             complement(1282..2475)
                     /locus_tag="PKH54_12045"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOD00670.1"
                     /translation="MVHTGLSILVEDALGYGSIPAVPRGARVGLVCHGASVDQHLRYT
                     FDLLAAAGYDLRFILAPEHGLFGDVPYMQRVEHSRLSHLGVPVVSLYGDSIEQLRPPP
                     GLLDGIDCLVCDIQDVGSRYYTYIATMAILMEVCSGTGIRFVVLDRPNPIGLDLVEGN
                     IIPPTMRSFVGYMQLPARHALTPGEAALHYRNVTGLDLDLQVVACRGLSRTMTWHDMD
                     LPWIPPSPNIPTPETALVYTGMCLLEGTNLSEGRGTASPFFLAGAPFITDPWGLVAEL
                     NRAMLPGVAFRPVRFTPAFDKWASKSCGGVHIVVTDEYEFKSLLTGMVLISTIARLYA
                     GQFSIRTHAYEFVSDTPALDLLIGDRTTGKALVMGVDAMDLYNDLQDRNHAWWDLVQD
                     VLLYR"
BASE COUNT          618 a          797 c          748 g          464 t
ORIGIN      
        1 atcccggccg cctgctggac cagggccgcc ggaaggttgg acacaagaac agccgcggcg
       61 tggtacaggt aacgatcctg atcatcaagt gcgaacggca cgaaacccat tgattcaagc
      121 aggtggatcg caaactcggg aagtccggat gtcccgaacg gcgttccgca cggcaccggc
      181 atcaaagtcg ccgcgggcgg gaaagggtga agagggtggg ccctgccgac actggcgccg
      241 aggtcttcag gcacgcgact tgtgtcccag gtcgcgctcg ccaccgcaat gcagtcggtt
      301 gcccgattga atccgggcga ctcgcaaatc tgtcggacaa ggtcgtcgtt gacgtgctcg
      361 ggcgtgcaaa ggtaccagaa agttggaacc gaagacggca ccacgaactt caccgacatc
      421 gcagcattca ttccgattgg aagaattgtg ggcatttgat acaccacgct ctgaacgtcg
      481 atgacccatg tcggtattgc cgcaagcacg catgaacgcg cgaaggaacc gccaacccgt
      541 ccgtgaccgc aaacgacgac cctgacttcg accgtcggcc ggtaaccagc gatttcatct
      601 tcccttgtca tttcgatcct ggtgccgctg tcagcccaaa gccgacatcc ggccatcggt
      661 tccgccccag gcgcgatgcc agccttcaag tcaggccata cagcccgaat ttttcaatgt
      721 aattcattac atcgccgtcc acggcatcat cacatcgatc acctgccgca atccggccgc
      781 gaacgcctga cgacgcaatc gccgggaaac tgcccagtcc gccaccggta aacccgcccc
      841 ggttaagaac gattggcggc gcgatcgccg taatccggtc aaaatccttc cagtcatcag
      901 tttcggcaag aatgtccgat ccgatgacaa ggcgaaggtt ttccgtggga tacatggcat
      961 gaagggtcgt aaccgtgttc actgtaaacg aaggcgtggg caacattgcc tcgatatccg
     1021 agacctcaac tgcggcgccg aactgaaaaa aggcaatccg gcacatctgg agccgatgct
     1081 cgaagtcggc catttccttg ccaaaggcat gggaaaatgc cggaattacg atgacccggc
     1141 cgcacccgcc gcgtgccaga agccaggata cggcctgcga atggcccagg tgcggcggat
     1201 cgaaacttcc accaaagact gccgtgaaac ccctcatatg cgatcacccg ttttcgcgcc
     1261 ggtcttgtcc ggcggacact atcatctgta aagcaggacg tcctggacca gatcccacca
     1321 ggcatgattc cgatcctgca gatcgttgta cagatccatc gcatcgacac ccatcaccag
     1381 cgcctttccc gttgtgcggt cgccaatcag cagatccagg gcaggtgtgt cgcttacgaa
     1441 ctcgtatgca tgcgtccgga tgctgaactg gccggcgtac aggcgggcga tggtggaaat
     1501 cagaaccatc ccggtcagaa gcgacttgaa ctcgtactca tcggtcacga cgatgtgaac
     1561 gccgccgcat gattttgaag cccatttgtc gaaagccggc gtgaacctta ccggcctgaa
     1621 tgcgacgcct ggaagcatcg cgcggttcaa ctcggccacc agaccccagg gatccgtgat
     1681 gaacggcgcc cccgcgagga agaacggtga tgcggttccg cgaccctcac tgaggttggt
     1741 gccctccagc aggcacattc ccgtatagac aagcgcggtc tcgggtgtcg gtatgtttgg
     1801 cgacggtgga atccacggaa gatccatgtc gtgccaggtc atggttcttg acagtccacg
     1861 gcaggcaacc acctggagat ccaggtcaag cccggtgaca ttgcgataat gaagcgccgc
     1921 ctcgcccgga gtaagcgcat gccgggccgg aagctgcatg tatccgacaa aagaacgcat
     1981 cgtcggcggg atgatatttc cctcgaccag atccagccca atcggattcg gtctgtccag
     2041 cacgacgaac cttatgccgg tgccggagca gacctccatc aggatggcca tggtcgcaat
     2101 gtatgtgtaa taccggcttc caacatcctg aatatcgcag accaggcagt cgattccatc
     2161 aagaaggccc ggtggcgggc gaagctgttc gatcgagtcg ccgtaaaggc tcacgacggg
     2221 cacgccaagg tgcgaaagcc ttgaatgttc aacccgctgc atgtacggaa catcgccgaa
     2281 aagcccgtgt tcgggcgcga ggataaaacg cagatcgtat ccggcggcag caagcaggtc
     2341 gaacgtgtat cgaagatgct gatcaacgga agcgccgtgg cagacaaggc cgacacgggc
     2401 gccgcgcggc acggccggaa tgctgccgta tccaagagcg tcctcgacca gaattgataa
     2461 tcccgtatga accatcgaag ccccgaatga aaccgaagcc cagaatactt caaaaaatcg
     2521 atccgacggt caaaaaaaaa ccccgccgaa gcggggtttt tcaactggcg gagtggacgg
     2581 gactcgaacc cgcggcctcc ggcttgacag gccggcgtta taaccga
//
LOCUS       DAOPPA010000464         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42720_length_2619_cov_4.431357, whole genome shotgun sequence.
ACCESSION   DAOPPA010000464 DAOPPA010000000
VERSION     DAOPPA010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42720_length_2619_cov_4.431357"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKH54_12050"
     CDS             <1..1078
                     /locus_tag="PKH54_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD00671.1"
                     /translation="RITEVLREDNAQIAPELRLIGSRAFALDYSPLVINEVDYNQSEY
                     NKSAQFVEFKNTTTRSLSLAGYALELYDTANLPVDFEHPKMTLNLSADVTLEPGGYYV
                     ICRSQAVSSLSSAECDRFAPNDMVILPWDGPRGGLRLVFGEGENMEVVDSLSWGQYVE
                     GLTEGTTPAPLDKTTKADYALSRVPDGLDTGENGFDFKWICASPRRANLTEGECACLQ
                     MSCGQGYACNPETLRCELVIVESDVIEDTGSSDTSTGEDTSVPPADAAGDGGDDLIPA
                     DSGPLPDIERDHGNVPDVPGVDTTVVREILVIQDLPNYGDNQTADNGGCSSGPGFPAT
                     GAPYGLLLLLFAAAAIFIGRRRVQ"
     gene            1172..2434
                     /locus_tag="PKH54_12055"
     CDS             1172..2434
                     /locus_tag="PKH54_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD00672.1"
                     /translation="MNRNLWIGFAVVTLAAVLGVMGWRIARAVADRKAAAVSVAVAPA
                     PVSVFQVAPRDIVSRVSMTGTIRPISEAPIFTKVPGRVQQVLVSVGDTVEPEQVLAVI
                     EHREIQLQADQANAGVKVAEAQMERARIGLDAATTAHDRLQGLRRDDAIPQSEFEKVE
                     YGLRDARASMLAAESGVATARAAAALARQMVENARVTTPIAGTVTHRFVNVGSPAGQT
                     MPVFQVQDLSVLKLDCNATARDFAKLAVGQQVNVTVPDLPGQVFPGTVLTLSPSLNPM
                     TRHAAVEISINNSEGRLLPNMFATAVIDAGTSESVPAVPSRAVLTAADGVVVFVVRDG
                     RAARVKPVLGGEDSGWLAVESGLAAGDMVIVSGHASLKDGDPVTVVDEPSPEPETPPE
                     PAAVKPDPSAAAPTDQAAPSPASGEAVR"
     gene            2431..>2619
                     /locus_tag="PKH54_12060"
     CDS             2431..>2619
                     /locus_tag="PKH54_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD00673.1"
                     /translation="MRMSEVAINRPVFTGMVALAVLVLGGLAFTRLGLGLFPHISIPI
                     VAVVVPYPGASPAEVESQV"
BASE COUNT          475 a          773 c          828 g          543 t
ORIGIN      
        1 acgtataact gaagtattac gcgaagataa cgctcaaatc gctccggaac ttcggcttat
       61 cggttcgcgg gcgttcgccc ttgactattc gcctcttgtc atcaacgagg tcgattacaa
      121 ccagtccgag tacaacaagt cggcgcagtt cgtggaattc aagaatacta cgacacgctc
      181 gctcagcctg gccggttatg cgctggagct ttacgacacc gccaacctgc cggttgattt
      241 cgaacacccg aagatgaccc ttaacctgtc ggcagacgtc acccttgaac ccggcggata
      301 ttacgtgatc tgccgttcac aggcggtgtc ttcgctttcg tcggccgagt gcgacaggtt
      361 tgccccgaat gacatggtca tccttccctg ggacggcccc aggggcggac tcaggctggt
      421 gtttggcgaa ggcgaaaata tggaagttgt tgattctttg tcatggggac agtacgtcga
      481 agggctgacc gagggcacga cgccggcccc gctggacaag acaaccaagg cggattatgc
      541 cctttcaagg gttccggacg ggcttgatac cggcgaaaac ggctttgatt tcaagtggat
      601 ctgcgcgtcg cccagaaggg cgaaccttac cgagggcgaa tgtgcctgcc tgcagatgag
      661 ttgtggccag ggttatgcgt gcaatcccga aacgctcaga tgcgaacttg tcattgtgga
      721 aagcgacgta atcgaggaca ccggttcgtc ggacacatcg accggtgaag atacgtcggt
      781 tccgccggca gacgccgccg gggacggtgg cgatgacttg attcctgccg attccgggcc
      841 gcttcccgac atcgaaaggg atcatggcaa tgtgccggac gtgccgggcg ttgacactac
      901 cgttgtccgc gaaatcctgg tgatccagga tctgccgaat tacggtgaca atcagacggc
      961 cgacaatggc ggttgttcgt ccggaccggg attcccggcg accggcgcgc catacggtct
     1021 tcttctgctt ctgttcgccg ccgctgcgat tttcatcggt cgccgacggg ttcaataacc
     1081 ccacctggag ggtgccgact tgcattgaca ggtcaagttg ttcggatgaa cattaaatgc
     1141 cttcggtttc ctggcggaaa ggggttcccg aatgaaccgg aatctgtgga ttggatttgc
     1201 tgtcgtcact ttggcggcgg ttcttggggt gatgggatgg cgtatcgccc gagcggtcgc
     1261 cgacaggaag gccgccgccg tttccgttgc ggttgcgccc gccccggttt ccgtttttca
     1321 ggttgcgcca cgcgatatcg tttccagggt ttcgatgacc gggacgattc gcccgatcag
     1381 cgaagccccg attttcacga aagtgccggg ccgcgtccag caggtcctgg tttcggtcgg
     1441 cgataccgtt gaacccgaac aggttcttgc ggtcatcgag catcgcgaaa tccagttgca
     1501 ggccgatcag gcaaatgccg gcgtcaaggt tgccgaggca cagatggaac gtgcaaggat
     1561 cggccttgat gcggcgacaa ccgcccatga caggcttcag gggcttcgtc gcgacgatgc
     1621 catcccgcag tccgagtttg aaaaggtcga atacggcctg cgcgatgcca gggcgtcgat
     1681 gctggcggcg gagtccggcg tcgcaacggc ccgggccgcg gccgctcttg ccaggcagat
     1741 ggttgaaaat gcacgggtga cgaccccgat tgcgggtacc gtaacccatc gcttcgtaaa
     1801 cgtcggttcc ccggccggcc agacgatgcc cgtattccag gttcaggatt tgtcggtgct
     1861 taagctggac tgcaatgcaa ctgcgcgcga cttcgcgaag ctggcggtcg gccagcaggt
     1921 taacgtgacc gtcccggatc ttcccggcca ggtttttccc ggcacggttc tgacattgag
     1981 ccccagcctg aacccgatga cgcgccacgc ggccgtcgaa atttcaatca acaattcgga
     2041 aggccggctg cttcccaaca tgttcgcgac ggcggtgatc gatgccggca cgtcggagtc
     2101 ggtgcctgcc gtgccgtcca gggcggttct tacggccgcc gacggcgtgg tcgttttcgt
     2161 cgtcagggat ggccgcgccg caagggtcaa gccggtgctc ggtggcgaag actccgggtg
     2221 gcttgcggtc gaatcaggcc tggccgccgg ggacatggtc atcgtgtcgg ggcatgcttc
     2281 gctgaaggat ggcgatcccg tgacggttgt tgatgaaccg tccccggaac ctgaaacgcc
     2341 cccggaaccg gcggccgtga aaccggatcc ctccgccgcc gcgcccaccg accaggccgc
     2401 ccccagtcca gccagcgggg aggccgtccg atgaggatgt ccgaagtcgc gatcaaccga
     2461 ccggtcttca ccggcatggt tgcgctggcg gttctggtac ttggcgggct ggcgtttacc
     2521 aggcttggcc ttggcctttt cccgcacata tcgatcccga ttgtcgccgt ggttgtcccg
     2581 tatcccggcg caagcccggc agaggtcgag tcccaggtt
//
LOCUS       DAOPPA010000465         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_42839_length_2614_cov_4.163736, whole genome shotgun sequence.
ACCESSION   DAOPPA010000465 DAOPPA010000000
VERSION     DAOPPA010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42839_length_2614_cov_4.163736"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2580)
                     /locus_tag="PKH54_12065"
     CDS             complement(<1..2580)
                     /locus_tag="PKH54_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOD00674.1"
                     /translation="MVRGAGFSNLRELYFGDRPAIAFSVASDDEVLAVTPPGRVGPSS
                     VRAVGEEGDVRADGIWAFDPVDVGPTMPDVAILSVSPAIGPVSGGQKIVIAGSGFFEG
                     LDVYVGPLPCTDVHYISDELIEAVTPPGTPGAADVTLSFRYMSVVSRGAYFFRGSDGG
                     TAGIFAVEPPDASWAGGSLVSVRGFGLSGIDTVWFGSNAGTIHRRVSDFEIIVRTPRV
                     SAAGRVFVMGLGKTGAMLPDGFRFYDPYLYGGGSRGRRISGDVQVTVYDGSTGARLSG
                     AYVIIGADLHTRLQGRTDDRGQITLSSEELAGAVDVSATATTYTAFTYAGVRGRDVTF
                     ALYATPPPETPTEPQPQEPSPTCTVSGRVRDFGKYFIKPQWLDGTAWVECFTSTSSMY
                     SSMSTLPESNRPREDGRFSIPARSGEFAVICRLMALETGMQQAYPLRMGVSRHVRCTD
                     GSVPDVELSLDIITESVARFGIVDAPAHHLGTWEPRFSGGWHLGSDGYLPLPYRGSFD
                     GGTVTFERQPDKLDGEGISDLAGTGYDLYATISSKDNTGMPYSVVQVTGLMPSSDGIL
                     LIGSPEDLRRVDTSIGRSFTTFERFSDGSALAVDDSGRTWVFDGSGFTAGRHVFSFKV
                     NDIAGESPDDFTAVGTRGRIVHVSEGVAEVVQTQFVWDFSSIDGPPGGPFSIVAGNYL
                     LRLENGIVTIENVPAGAVMSTVRRFEDGTIFAAGPDGLIVTGTSQSPLAAVRPVAEDL
                     YAVAGESPLRVWMAGENGRVILIRDGDVTVFKTPGSEALRGILELGECNVLFFGDSGA
                     LYQYDCFRFNDISDRTSMPYAISRGVVLDAAAGLVAMVSPPVNMLPGFAGFPEVSMPV
                     DGMA"
BASE COUNT          574 a          832 c          792 g          416 t
ORIGIN      
        1 ggccatgccg tcgacgggca tcgatacttc ggggaaaccc gcaaagccgg gcagcatgtt
       61 gaccggtggc gaaaccatgg cgaccagtcc tgccgccgca tcaagaacga cgcccctgga
      121 aatcgcgtac ggcatggatg tcctgtccga aatgtcgttg aaccggaagc agtcatactg
      181 atacagggct cctgaatcgc cgaagaaaag tacgttgcac tcacccagtt caagtatgcc
      241 cctcaaggcc tcgctccccg gcgtcttgaa aacggtaacg tcgccgtccc tgatgaggat
      301 cacgcgtccg ttttccccag ccatccaaac gcgaaggggg gattccccgg ccacggcgta
      361 caggtcttca gcaaccggcc tgaccgcggc gaggggcgac tggctggtgc cggtgacgat
      421 caggccgtcc ggaccggccg caaagatggt gccgtcctcg aagcggcgaa cagttgacat
      481 gacggccccc gcgggaacgt tttcaatcgt gactatgccg ttttcaaggc gcagcaggta
      541 gtttcccgcg acgatgctga acggtccgcc cggggggccg tcaatcgagg agaaatccca
      601 gacgaactgg gtctgaacca cctcggccac gccctccgag acatgaacga tccggccacg
      661 cgtcccgact gccgtgaagt cgtcgggaga ctcgccggcg atgtcgttga ccttgaagct
      721 gaacacatgc cgccccgcgg tgaagccgct gccgtcaaat acccaggttc gccccgaatc
      781 atcaaccgcg agcgcggatc cgtcggaaaa gcgctcgaat gtcgtgaaag aacgcccgat
      841 cgacgtatct accctccgta aatcctcagg cgaaccgatc agcaggatac cgtccgaaga
      901 aggcatcagg ccggttacct gaaccacgga atacggcatg ccggtgttgt ccttggacga
      961 aatggtggcg tacaggtcgt atccagtgcc cgccagatcg gaaatccctt cgccgtcaag
     1021 cttgtccggc tgacgttcaa acgtcaccgt tccgccgtcg aagctccccc tgtacggaag
     1081 cggaagatag ccgtcggaac caaggtgcca tccaccggaa aagcgcggct cccatgttcc
     1141 caggtgatgc gcgggggcgt cgacaatccc gaacctggcg acggattcgg taatgatatc
     1201 aagagaaagc tcgacatccg gaacgctgcc gtcggtacac cggacgtgcc tgctgacgcc
     1261 catccgcaag ggatatgcct gctgcatgcc ggtttccagg gccatcagcc gacatatgac
     1321 cgcgaactcg ccgcttcggg ccgggatcga aaagcgacca tcctcgcgcg gtcggttgga
     1381 ctcgggaagc gtgctcatcg aggagtacat ggacgacgtc gatgtgaagc actcgaccca
     1441 ggcggttccg tcaagccact ggggcttaat gaaatacttc ccgaaatcgc gcacccgacc
     1501 ggataccgta cacgttggcg aaggttcctg gggctgcggc tccgtcggcg tttcgggcgg
     1561 cggggtagca taaagcgcga aggttacatc gcgaccgcgc accccggcgt acgtgaaggc
     1621 ggtgtaggtc gtggccgtgg ccgatacgtc aaccgcaccc gcaagttcct cggaggaaag
     1681 cgttatctgg ccgcgatcat cggttcggcc ctgaaggcgc gtatgcagat cggcgccgat
     1741 tatcacgtat gcgccggaaa gcctggcgcc ggttgacccg tcatagacgg taacctgtac
     1801 gtcgcccgaa atgcgacgcc ccctcgaccc gccgccatat aaataaggat catagaaccg
     1861 gaagccgtcc ggcagcatcg cgccggtctt tccaagcccc atgacaaaga ccctgccggc
     1921 ggctgaaacc ctgggggttc ggacaatgat ttcgaagtcg gaaacgcgcc gatgtatggt
     1981 gccggcattg gaaccgaacc agaccgtgtc gatcccggag aggccgaagc cgcgaaccga
     2041 tacgagcgaa ccgccggccc aggacgcgtc gggcggttcg acggcgaaga tcccggccgt
     2101 cccgccatcg gaaccgcgga agaaataggc gccgcggctg acgacagaca tgtatcggaa
     2161 cgaaagggtt acatcggccg caccgggagt tcccggcggg gtgaccgcct cgatgagttc
     2221 atccgatatg taatggacgt cggtacatgg cagcggcccc acgtaaacgt caaggccctc
     2281 gaaaaatccg cttccggcaa tgacaatctt ctggccgccg gacaccgggc cgatcgccgg
     2341 agaaacggaa agaatcgcga catccggcat ggtcggcccg acatcgaccg gatcgaaagc
     2401 ccagatgcca tcggccctga catcgccttc ttcgccgacg gcccttaccg atgacggacc
     2461 gacccgtccc gggggcgtga cggcaagcac ctcgtcgtca gacgcgacag aaaacgctat
     2521 cgccgggcga tccccgaaat agagttcacg aagattggaa aatcccgcac cgcgaaccac
     2581 cacaggggat ccgccaaccg aagggccgga cgcc
//
LOCUS       DAOPPA010000466         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_43126_length_2601_cov_3.902592, whole genome shotgun sequence.
ACCESSION   DAOPPA010000466 DAOPPA010000000
VERSION     DAOPPA010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43126_length_2601_cov_3.902592"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKH54_12070"
     CDS             complement(<1..266)
                     /locus_tag="PKH54_12070"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180251.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HOD00675.1"
                     /translation="MAVSPLAGKPVPPELVEDVDSVVSAYHSVRPDPGTPAQAVSFGT
                     SGHRGRSVDGTFNEDHILAVTQAVCEYRASRGFDGPLFIGMDSHA"
     gene            complement(348..1292)
                     /locus_tag="PKH54_12075"
     CDS             complement(348..1292)
                     /locus_tag="PKH54_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00676.1"
                     /translation="MTKILVLFVALASMVGCGGGIGVDGDVFIPLPDMWLNDGAGDGL
                     AIDVAPDGQIQPDSVRTDLPDATSGDEGDNTDEGPVDSGADHGQPPDDVVDNDADKTD
                     VAATDLNQPDVPRCFLGQDECLPDSSTNRKTRPDAQILGRFTLLNGGISWNSDTINEN
                     SGGNEDDDDVSTKGCPIFGGGDDLWCKSECDDDGLDHFFRVFLVAGDLFSFTVDDHLI
                     YNDEDWGIHIDFMVKVYRGWTCPLERESLLQCVNQSDGNRPTYNNVQIKPMSESEAGW
                     YTIVIDSTGSDHAGTYSLSASLFQNSEYTGDWNLCCDF"
     gene            1563..>2601
                     /locus_tag="PKH54_12080"
     CDS             1563..>2601
                     /locus_tag="PKH54_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00677.1"
                     /translation="MKKLEIVLAASILSLCVMIPRAALPCGPGVHIRESDRIWSKLTA
                     EFPAFKDLEDTPLFSTYLHLGSIMPDFQWFLPIHQGHSSEFSQYLVQMSMDQDSRFQI
                     LALGHLMHNSVSDPLCEQFWTPTLMASAPLGMVSLLEGYADAHGMAEGVTEVLGDPIV
                     GDWDALIDAIFDVYLDGPEAEALGHEAITWYVDSMNAYYGSDTDGEAVWQGLVDKITE
                     YQSYLAGFDRQAAKQFFHELRGETPADMMNLLTGDLIAKVAGDAAKRNQDYDLNIGFV
                     MQSAIVDQAFWDIYQDTLLADLSSFWYIDRLNSFAETLLPDDASTMILGWPSWNHWPM
                     VSGNIMSTMQFL"
BASE COUNT          550 a          797 c          721 g          533 t
ORIGIN      
        1 gcatgggagt ccatgccgat gaacagcggg ccgtcgaacc cccgcgaggc ccggtactcg
       61 cagacggcct gtgttaccgc aagaatgtga tcttcgttga aggtgccgtc aaccgatctg
      121 cccctgtgac ccgaagtgcc gaacgacacg gcctgagccg gggttcccgg atcggggcgg
      181 acggaatggt acgcactgac gaccgaatcg acatcctcga caagctccgg tggcaccggc
      241 tttccggcaa gcggtgaaac tgccatgaaa cgacctcgat tgcgacggcc gcattatatg
      301 atgaaatcac aggtacggga cggatgcgtt tccggcggaa ccgacaatca gaagtcgcag
      361 cacaggttcc agtcgccggt gtactcggag ttctggaaca ggctggccga aagcgaatag
      421 gtgccggcgt gatcgcttcc ggtgctgtca atgacgatcg tgtaccagcc ggcctccgac
      481 tcgctcatcg gcttgatctg gacgttgttg tacgtcggtc ggttgccgtc cgactggttc
      541 acgcattgaa gcagcgattc gcgttcaagc ggacaagtcc agccccgata gactttcacc
      601 atgaagtcga tatgaattcc ccagtcttca tcgttgtaaa tgaggtgatc atcaacagtg
      661 aaactgaaca gatccccggc caccaggaaa accctgaaaa agtgatccag gccatcatcg
      721 tcgcattccg acttgcacca caaatcgtcg ccgccaccaa aaatcgggca gcccttggtg
      781 ctgacgtcat catcgtcttc gttaccgccg gaattttcgt taatggtgtc gctgttccag
      841 gaaatcccgc cgttcaacaa agtgaaacgg ccaaggatct gggcgtccgg gcgggtcttc
      901 ctgttggttg acgagtccgg caggcactcg tcctggccaa ggaagcaccg cggcacatcc
      961 ggctgattca aatcggtggc cgcgacatcc gttttgtcgg cgtcgttgtc aacgacgtcg
     1021 tccggcggct ggccgtgatc tgcccccgaa tcaaccgggc cttcgtcggt attgtcacct
     1081 tcgtcacccg aagtcgcgtc cggcagatcg gttcgaaccg aatcgggctg aatctggccg
     1141 tccggcgcca catcaatcgc caaaccgtcc cccgcgccat cgttcagcca catgtctggc
     1201 agcgggatga atacatctcc atcgacacca attccgcccc cgcaccccac catcgacgca
     1261 agcgccacaa aaagaacaag gatctttgtc atcggcacac ccagtcttcg aacacggcca
     1321 tcaaggcggc ggcacattcc ggccccgacg gaggctggaa gtgtaggtga aacgaacgga
     1381 tttttcaccc gcaaccgcgc cgtcagctcg caaccaaccg aatatcaagt aatttcggcc
     1441 aggaagatcc gatcgaccat cactcccgcg tcaatgccga ttgcgtgctg acagcattga
     1501 ccgggagtcc tataatcttg tccggcgtca atcccggtca ttcccggaaa aggtgattca
     1561 gcatgaaaaa actggaaatt gtcctggcgg catcgatctt gtcgctgtgc gtcatgattc
     1621 cacgggcggc cctgccgtgc gggcccggcg tccacatccg ggaatccgac cggatatggt
     1681 cgaaactgac tgccgaattc ccggccttca aggatctgga ggatacgccg ctgttttcga
     1741 cgtatctgca cctgggttcg atcatgccgg acttccagtg gttcctgccg attcaccagg
     1801 gccactcgtc ggagttttca cagtatctgg ttcagatgtc gatggatcag gattccaggt
     1861 tccaaatcct ggcccttggg cacctgatgc acaactcggt ttcggatccg ctctgcgaac
     1921 agttctggac cccgacactg atggcttcgg cgccgcttgg catggtcagc cttctggaag
     1981 gatacgcgga cgcgcacggc atggccgaag gcgtcaccga agtccttggc gacccgattg
     2041 tcggcgactg ggacgccctg atcgatgcca tcttcgacgt ttacctggac ggccctgaag
     2101 ccgaggcgct tggtcacgag gcgattacat ggtacgtcga ttcgatgaac gcctattacg
     2161 gttccgacac cgatggcgag gccgtgtggc agggcctggt cgataagatc accgaatacc
     2221 agtcatacct cgccggtttc gaccgccagg ccgccaaaca gttcttccac gaacttcgcg
     2281 gcgaaacccc ggccgacatg atgaacctgc ttaccggcga cctgattgca aaagtcgccg
     2341 gcgatgcagc gaagcgcaac caggattacg acctgaacat cgggtttgtc atgcagtccg
     2401 caatcgttga tcaggccttc tgggacatct atcaggatac cctcctggcc gacctgagca
     2461 gtttctggta catcgatcgg ctcaactcgt tcgccgaaac actcctgccg gacgatgctt
     2521 caacgatgat tcttggctgg ccgtcgtgga accactggcc catggtgtcg ggcaacatca
     2581 tgtcaaccat gcagttcctg c
//
LOCUS       DAOPPA010000467         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_43260_length_2594_cov_4.270185, whole genome shotgun sequence.
ACCESSION   DAOPPA010000467 DAOPPA010000000
VERSION     DAOPPA010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43260_length_2594_cov_4.270185"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1866
                     /locus_tag="PKH54_12085"
     CDS             193..1866
                     /locus_tag="PKH54_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOD00678.1"
                     /translation="MTEASMFKEHPRGLIVAFFANMGERFGFYTMMAILVLFLQARFG
                     LDSDKSGAIYSVFYFLIYALALVGGIIADKTRNYKRTILAGIIIMFLGYVLMAIPGTG
                     LPIALTGLFTIALGNGMFKGNLQAVVGQMYDDPKYNHLRDSAFSLFYMGINIGAVFAP
                     NAARSVRTWYLGTQGLKYDADLPALCHQFVAGNTDNASRLQEIASLVSGGQVSDLGAF
                     ARNYLEVFSTGYNMAFGIAAGAMLISLATFVIFKKFLPDPKAKARPGEAGEAMPWDAE
                     KKRLMALGLVFLVVIFFWMSFHQNGLTMTFFARDYTAKSVDPLSYLVFDIKGLLLIAA
                     MIAGVVVAIQKGTVVRRLVGSGMAVAGAVLIWFLYGSYKPENLLEPEVFQQFNPYFVV
                     FLTPVVVGLFALLRARNREPSTPKKIGIGMLLAATGFTLLMLASFGLPSAAELGGGPS
                     PDRVSPNWLITTYFILTVAELFLSPMGLSFVSKVAPPRFQGLMQGGWLGATALGNQLL
                     VVGSLMWARFDVWTVWAVFVVCCLISATVIFSMSGWLNRMTGAGSPKAQ"
     gene            1949..>2594
                     /locus_tag="PKH54_12090"
     CDS             1949..>2594
                     /locus_tag="PKH54_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOD00679.1"
                     /translation="MAVLLALVVAVLWGFGNVFQKHGMSTSFPKISLKNVLRQIFPIV
                     KTLFTNWLWLVGTVMMATGAGLYFVALGYGDVTIVQPILCLTVAVSAVVGVVWLKERV
                     SGIEWVGIVLVLAGVILVSVAGGGKTAVLPEGFGLAVFFAAIASLAAAAFLLGRIGIS
                     IEFSLAVSAGLVFGLANVAAKLMSMRFEAEIGSPNLFQLAAVVSILTDYPIVLVV"
BASE COUNT          427 a          762 c          774 g          631 t
ORIGIN      
        1 tagctcgtcg ggctcataac ccgaaggcca caggttcaaa tcctgtcccc gctaccattt
       61 caacagatca ggcgcctttg ggcgcctttt ttgtatccgg tgcgtcccgg ttcgaattgt
      121 cttgaaccaa tcggaatccc ctgcaagaat tccctttccg cggtccggat gttccggaac
      181 cgccaccgca gcatgacgga ggcatcgatg ttcaaggagc acccccgggg actcatagtc
      241 gcattcttcg ccaacatggg tgaacgcttc ggcttttaca cgatgatggc gatcctggtc
      301 ctgttccttc aggcaaggtt cgggcttgat tcggacaagt ccggcgcgat ttacagcgtc
      361 ttttacttcc tgatttacgc gctggcgctg gtcggcggca tcatcgcgga caagacccgc
      421 aattacaagc ggaccatcct ggccggcatc atcatcatgt tcctgggtta cgtcctgatg
      481 gcgatacccg gcaccgggct tccgatagcg cttaccggcc tcttcacgat tgccctggga
      541 aacgggatgt tcaagggcaa tctccaggcg gtcgtcggcc agatgtacga tgatccgaag
      601 tacaaccacc ttcgcgattc ggcgttctcg cttttttaca tgggcatcaa catcggcgcc
      661 gttttcgcgc cgaacgccgc gcgcagcgtc cgcacctggt accttggcac ccagggtctg
      721 aagtatgacg ccgacctgcc ggcgctgtgc caccagtttg tcgcaggcaa caccgataac
      781 gcttcccggc tgcaggaaat cgccagcctc gtctcgggcg gccaggtttc cgatctcggg
      841 gctttcgcca ggaattacct ggaagtcttc tcgaccggtt acaacatggc tttcggcatc
      901 gcggccggcg ccatgctgat ttcactggcg actttcgtga tcttcaagaa gtttctgccg
      961 gacccgaagg ccaaggcccg tccgggcgag gcaggcgagg cgatgccatg ggatgccgaa
     1021 aagaagcgcc tgatggcgct gggactggtg ttcctggtcg tcatcttttt ctggatgtcg
     1081 ttccatcaga acggcctgac aatgacgttc ttcgcccgcg attacaccgc caaatcggtt
     1141 gatccgctga gctatctggt cttcgacatc aaggggcttc tgctgatcgc cgcaatgatt
     1201 gccggcgtcg tggtcgcgat tcagaaggga acggtggttc gccggctggt aggatccggc
     1261 atggccgtcg cgggtgccgt gctgatttgg ttcctttatg gctcgtacaa gccggaaaat
     1321 cttcttgaac cggaagtctt ccagcagttc aacccgtatt tcgtggtttt cctgactccg
     1381 gtagtcgttg gcctgttcgc cctgcttcgt gcccggaacc gcgagccatc gaccccgaag
     1441 aagattggga tcggcatgtt gctggccgcc accggcttca ccctcctgat gcttgcgtcc
     1501 ttcggcctgc cgtcggccgc ggaactgggt ggtggtccct cacccgacag ggtttcacca
     1561 aactggctga tcacgactta cttcatcctt acggttgccg agcttttcct gtctccgatg
     1621 ggcctgtctt ttgtttccaa ggtggccccg ccaagattcc agggattgat gcagggcggc
     1681 tggcttggcg ccacggctct tggcaaccag cttctggtcg tcggcagcct gatgtgggcg
     1741 cgtttcgacg tctggacagt atgggcggtt tttgtcgtct gctgcctgat ttcggcaacc
     1801 gtcatctttt cgatgtccgg ctggcttaac cggatgaccg gtgcaggaag cccgaaggcg
     1861 cagtaatccg ttctgttgcc gaactgtcaa atccactcat acggtcttac aataggccgc
     1921 cgaccgtttt tcccggggga aatgaataat ggcagtgttg ctggcgcttg tggttgcagt
     1981 cctgtggggc ttcggtaacg tctttcagaa gcacgggatg tccacgagct tcccgaagat
     2041 ttcgttgaag aacgtgctgc gtcagatctt tccgattgtg aagacgctgt ttacgaactg
     2101 gctctggctg gtcggcaccg tgatgatggc aactggggcc ggcctgtact tcgtggccct
     2161 gggatacggc gacgtgacga tcgttcagcc catcctctgc ctgacggttg ccgtttcggc
     2221 agttgtcggt gtcgtctggc tgaaggaaag ggtcagcgga atcgagtggg tggggattgt
     2281 gctggtgctt gccggcgtga tcctggtcag cgtcgcgggc ggcgggaaga ccgccgtgct
     2341 tccggaagga ttcgggctgg cggtattttt cgccgccatc gcctcgcttg ccgcggccgc
     2401 cttcctgctt ggcaggatcg gcatcagcat cgagttttcg ctggccgttt cggccggcct
     2461 ggtattcggc ctggccaacg tcgccgccaa gttgatgtcc atgcgcttcg aggccgagat
     2521 cggcagcccg aacctgttcc agctggcggc cgttgtcagc atcctgaccg attacccgat
     2581 tgtcctggtc gtca
//
LOCUS       DAOPPA010000468         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_43986_length_2562_cov_2.933785, whole genome shotgun sequence.
ACCESSION   DAOPPA010000468 DAOPPA010000000
VERSION     DAOPPA010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43986_length_2562_cov_2.933785"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PKH54_12095"
     CDS             complement(<1..1524)
                     /locus_tag="PKH54_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00680.1"
                     /translation="MTRINIRDVRRAPGFAIQATTFGVLAALLVITGCDGTSGPGADF
                     DLTDEPAGTNLSGIDMSGYSQVGQPTPAGGFVQFPDGTGIQVSPGLSDGSIIIGLRPS
                     ADGPVPGNDVQGIALTPWIDAAVSTSNFDATRDAPFLIQVPVTPPDGSIDHPGLQLTL
                     VLPDGSAFPINGTFDSGFGVFNAAMLALPPSFQFAVTFNEAITRIDSADVPELISGQF
                     AQSALTEGPWSTTEFVIDFDGNKVTMEQARKVARAARRAAATYSAAGFGEPFLFKETG
                     ILGERWHIHLTAKGSCFDSNHDSAAEDISRRFGRLFVSTARIDSPTTDNLGSVYASIA
                     HEMFHSIFFNYEIPYVCFNYVKNGTTYCHRSYDGFNEGMATTIGYSLDQGQVKPRPNE
                     APNPLVVPIGYFNSNERWVSYRNQDFFVFLLRIASLSNVEKMLRTLKTAVMPATPTNT
                     MACLTAYGKALEAGGVGLDIGFTELLGGYVANRGFIREPEGHIWPAEPSGGQPGEMYA
                     "
     gene            complement(1631..>2562)
                     /locus_tag="PKH54_12100"
     CDS             complement(1631..>2562)
                     /locus_tag="PKH54_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00681.1"
                     /translation="IEWIASQGFNVVRVPMTYRNLVTGPDLDAPTELSWNELAWNRIS
                     MLLDWCEKHRIYAVLDIQEAPGGQNDYTGQSLLYGSPALQDLTVELWVEIATRFGGHS
                     AVAAYSLLAEPFSAPDPGARDAMYDKLYDAIRALGDNHLLVIHDGFFGMNTLPDPAAY
                     GWENVVYSTHIFEFDAKSFEDYEFLINIFHETVYTEAQAAQGVPYYIGSFSTRNDADW
                     AYDAAQLMIDWYTGHGWSWSVWTYKRIDDPIAFELLGKTSSYGVRTMHEGPFDRPDAF
                     DDDFDTHKAKLAAYRDLPLDPNQRLLDILTSPM"
BASE COUNT          509 a          696 c          851 g          506 t
ORIGIN      
        1 cgcgtacatc tcgccgggct ggcctccgct tggttcagcg ggccagatgt ggccctcggg
       61 ctcgcggatg aagccgcggt tcgcgacata cccgccaagc agctcagtga agccgatatc
      121 cagtcccact ccgcccgcct cgagtgcctt gccgtaagcg gtcaggcatg ccatggtgtt
      181 cgtcggggtt gccggcatta cggcggtctt gagcgtgcgc agcatctttt caacgttgga
      241 aagcgacgcg atgcgcagca ggaatacgaa gaaatcctga ttgcggtacg acacccaccg
      301 ttcatttgag ttgaagtacc cgattggcac aaccagagga ttcggcgcct cgttcgggcg
      361 cggtttcacc tggccctggt caagcgaata gccgatcgtc gtggccatgc cctcgttgaa
      421 gccgtcgtaa gagcgatggc agtaagtcgt gccattcttc acgtagttga agcagacgta
      481 gggaatttca tagttgaaga agatggaatg gaacatttcg tgggcgatcg aggcatagac
      541 ggagcccaga ttatcggttg tcggcgaatc gattcgggca gttgacacga aaagacggcc
      601 gaaccgtcgc gagatgtcct cggcggccga gtcgtggtta ctgtcgaagc agcttccctt
      661 tgcagtcagg tggatatgcc agcgttcccc aagaatcccg gtttccttga acagaaatgg
      721 ttcgccgaag ccggcggcag agtatgtggc cgcggctctg cgggcggcgc gcgcgacctt
      781 gcgggcctgt tccatggtca ccttgttccc gtcgaagtca atcacgaatt cggtcgttga
      841 ccatgggccc tcggtcaggg cggactgggc gaattgtccg gaaatcagtt ccggcacgtc
      901 ggcactgtcg atgcgggtta tcgcttcatt gaacgtaacg gcgaactgga aactgggcgg
      961 cagggcaagc atggccgcat tgaagacccc gaaccccgaa tcgaatgtgc cgtttatcgg
     1021 gaacgcgctg ccgtccggca ggacaagtgt cagttgaagg cctgggtggt cgatcgaacc
     1081 atctggcggt gtgaccggaa cctggataag gaagggggca tcccgcgtgg catcgaagtt
     1141 ggatgtgctg acggccgcgt cgatccacgg ggtcagtgcg attccctgga cgtcgttgcc
     1201 gggaacagga ccgtcggcgg acggacgcag gccgatgatg atcgaaccat cggacaagcc
     1261 aggcgaaacc tggatgccgg ttccgtcggg aaactgcacg aagccgccgg cgggggtcgg
     1321 ctggccaacc tgggagtatc ctgacatatc gatcccggaa aggttcgtgc cggcgggctc
     1381 gtcggtcagg tcgaagtccg ccccggggcc ggatgtgccg tcacagccag taataacgag
     1441 caaggcagcc aggacgccaa aagtcgttgc ctggatcgca aaaccgggag cgcggcgcac
     1501 gtcgcgaatg ttgattctgg tcatgtaaat accggaaagg gatttgtaaa gattctagcg
     1561 gggtgcgcgg gagaggtaaa gtgcgtgccg gtgaatcccg accgtcatga cctgagaacc
     1621 tgccgaatcg ctacatcggg gatgtcagaa tgtccagcag gcgctggttc gggtcaaggg
     1681 gcaggtcgcg gtaggccgcc agtttggcct tgtgtgtgtc gaagtcgtcg tcgaacgcgt
     1741 ccgggcggtc gaacgggcct tcgtgcatgg tgcggacgcc ataactgctg gtcttgccaa
     1801 gaagctcgaa cgcaatcggg tcatcgatgc gcttgtaagt ccagaccgac caggaccagc
     1861 cgtgtccggt gtaccagtcg atcatcagtt gcgcggcgtc gtatgcccag tcggcgtcgt
     1921 tgcgcgtgga aaagctgccg atgtagtacg ggacgccctg ggcggcctgg gcctcggtat
     1981 agacggtttc atggaagatg tttatcagga attcataatc ctcaaaggac ttggcgtcga
     2041 attcgaagat gtgagtggaa tagacgacgt tttcccagcc gtacgcggca gggtcgggca
     2101 gcgtgttcat accgaagaag ccgtcgtgga tgacaagcag gtggttgtcg ccaagcgcgc
     2161 ggattgcgtc gtacagcttg tcgtacatgg cgtcacgggc gccggggtca ggcgcgctga
     2221 agggttcagc cagcagcgag taagccgcga cagcgctgtg tccgccgaat cttgtggcga
     2281 tctcgaccca caattccacc gtcagatcct gcagggcggg gctgccgtac agaagcgatt
     2341 gaccggtgta gtcgttctgg ccgccgggag cttcctggat gtcaagaacg gcatagatcc
     2401 tgtgcttttc gcaccagtcc agcagcatgc tgatgcggtt ccaggcaagc tcgttccagc
     2461 tcaactccgt gggtgcgtcc aggtcggggc cggtcaccag gttgcggtag gtcatgggca
     2521 cgcgaaccac gttgaaaccc tgggacgcaa tccactcgat gt
//
LOCUS       DAOPPA010000469         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_44493_length_2541_cov_4.218021, whole genome shotgun sequence.
ACCESSION   DAOPPA010000469 DAOPPA010000000
VERSION     DAOPPA010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44493_length_2541_cov_4.218021"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKH54_12105"
     CDS             <1..1078
                     /locus_tag="PKH54_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD00682.1"
                     /translation="LGLLAGLPTDRPTLVTGWHSGRLRIGPDDLHGLPPVVIINFSLH
                     GYMMNDAAAGVAERAGFPVVPASAHEAERLMPDVLTFFGGFCPPDAEMAERFVNRMKR
                     IGLYGMEDMLRLPGLKADLPERPDFKIRSWITPDSFYDGCWEGGRLSGFKLFADGALG
                     SGTAAMSSGFGQGWTSVLTHSDRELREKVAWCLDNGPGLAVHAIGDLAIDQILRVLGG
                     VAPTVRPRVRLEHVQFIDFRQAMTAREMGLTLSMQPNFSEDSIAYADRLDGRTLSKNN
                     PFRMLIDDAGFTPGRDLLFGSDGMPHGIAAALQNGLFPAVECQALSIDELLAGYSADL
                     SHGCVQVDIDTQARRVLLVDGGSD"
     gene            1213..1938
                     /gene="rph"
                     /locus_tag="PKH54_12110"
     CDS             1213..1938
                     /gene="rph"
                     /locus_tag="PKH54_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOD00683.1"
                     /translation="MPRPDARGDDQLRPVSFTLGVQRGPAGSVMITQGNTVVLCAATV
                     ADKVPGWLAAKGRGWVTAEYSMLPGSVSDRAPRKQGGRDQEIQRLVGRSLRAAVDLER
                     LGERLITIDCDVIQADAGTRVASITAGWIALRLAVDGLLRSGGLKVDPIIRQVAAVSV
                     AVVDGWAVLDPVYEEDVDAQVDANFVMSSDGLLIEVQSTAEGLPFSRAMLDTMLDLAG
                     TGTAALFAAQTAALTIRRSTLDE"
     gene            complement(2112..>2541)
                     /locus_tag="PKH54_12115"
     CDS             complement(2112..>2541)
                     /locus_tag="PKH54_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00684.1"
                     /translation="LFTAGFVAHAAIWPSGNHNVVTIVKMSGLMVGVVGFLITYQLYL
                     NVFDYRPIRIWELRPQMLLSRKTTDTLKEDRDMLDHKMGPIGPDGLSRQQVETLRRWW
                     IDRGKGGKIWISRTIPFAPALFIGTILTVILSGYIFRVTY"
BASE COUNT          441 a          723 c          841 g          536 t
ORIGIN      
        1 ccttggactt cttgcagggc tgccgaccga ccggccgacg cttgtgaccg ggtggcacag
       61 cgggcgactg cggatcgggc cggacgatct tcatggcctt ccgccggtgg ttattatcaa
      121 tttcagtctt cacggctaca tgatgaacga cgcggcggcg ggggtggcgg aaagggccgg
      181 tttcccggtg gttcccgcgt cggcacacga ggccgagcgg ctgatgcccg acgttctgac
      241 gttcttcggt ggcttttgtc cgcccgacgc cgaaatggcc gaacggttcg tcaatcggat
      301 gaagcgaatc ggattgtacg gcatggaaga catgctgagg cttcccggac tcaaggccga
      361 cctgccggaa aggccggatt tcaagatccg ttcctggatt acaccggatt cattttatga
      421 cggctgctgg gaaggaggcc gtctttccgg cttcaagctg tttgccgacg gcgcgctcgg
      481 gtcgggaacg gcggcaatgt cttccggttt cggacagggc tggacgtctg tcctgacgca
      541 ttccgaccgt gaactgcggg aaaaggtcgc gtggtgtctt gataacggtc cgggacttgc
      601 ggttcacgcc atcggggatc ttgcaatcga ccagattctg cgggttcttg gcggcgtggc
      661 cccgaccgtt cgtcccaggg tcaggctgga gcatgtccag ttcatcgatt tcaggcaggc
      721 gatgaccgcc cgtgaaatgg ggctgactct gtcgatgcag cccaacttca gcgaggattc
      781 aatcgcctat gccgatcgtc ttgatgggag aacgctgtcg aaaaacaacc cgttcaggat
      841 gttgattgac gacgccggct tcacgccggg ccgggatctg cttttcgggt cggatggaat
      901 gccgcacggc attgcggccg ctttgcagaa tggcctgttc cctgcggttg agtgccaggc
      961 cctgtcgatt gacgaacttc tggccggata ctccgcggac ctgtcccacg gttgcgtgca
     1021 ggtcgatatc gacacgcagg cgcgacgggt gttacttgtg gacggcgggt cggactgatt
     1081 gccgatgccc ggtgtcccgg ggggctcttg ccatccctgc atcggtgcga tggctggact
     1141 cgaaagtgtc gctttgtgaa tggaactgac gcaggcgccg gtatcgccgt cgcaacttga
     1201 gatgggggtt ttatgccaag accggatgca aggggggacg accagcttcg tccagtatcg
     1261 tttacgctcg gggttcaacg cgggcctgcg ggctcggtga tgataaccca gggaaatacc
     1321 gtcgttcttt gcgcggcgac cgtcgcagac aaggttccgg gctggcttgc cgcaaagggc
     1381 aggggatggg ttaccgccga gtactccatg cttcccggtt cggtctccga ccgggcgccc
     1441 cggaagcagg gcgggcggga tcaggaaatc cagaggcttg tcggtcgcag cctgcgggcc
     1501 gcggttgacc tggaacggct gggggaacgt ctgattacca tcgactgcga cgtcatccag
     1561 gccgatgccg gaaccagggt tgcgtcaatc accgcgggat ggattgcgtt gcggctggct
     1621 gttgacgggc tgctgcgttc gggtggcctg aaggtggatc cgatcattcg ccaggtcgcg
     1681 gccgtttcgg tcgcggtcgt cgatggttgg gcagtcctgg atcccgttta tgaagaggat
     1741 gtcgatgccc aggtcgatgc taatttcgtc atgtcgtcgg acgggctcct gatcgaggtt
     1801 cagtcaaccg ccgagggcct gccgttttca cgcgccatgc tcgacacgat gctggatctg
     1861 gccggaaccg ggacggcagc gttgttcgcg gcccagaccg ccgccctgac gatccggcgt
     1921 tccacgctcg acgagtgacg cagtcgtgcc tgtggccggc catgaagtgt ccggttcgag
     1981 gggccaaacc ggagcactta tgatccggcc cgatccgccc cccgatccgt acgggcgaat
     2041 gaaaattcgc ccgcctgcga tcacgccgtg ggtttgggga agttggagcg ttcgtgattg
     2101 accctgggaa atcagtatgt gacccggaag atgtaaccgg acagaatcac ggtaagaatg
     2161 gttccgatga aaagcgccgg cgcgaacggg atcgtccttg aaatccagat ttttccgccc
     2221 ttgccgcgat caatccacca ccgtctaagc gtttcgacct gctgcctgct gagcccgtcc
     2281 ggcccgatag ggcccatctt gtgatccagc atgtcgcggt cttccttcag cgtgtcggtg
     2341 gtcttcctgg acagaagcat ctgaggtcga agctcccaga tccggatcgg ccggtaatcg
     2401 aacacgttca ggtaaagctg gtatgtgatc aggaacccga cgacgccgac catcagcccc
     2461 gacatcttaa cgattgtcac gacgttgtgg ttcccgcttg gccagatggc cgcgtgtgca
     2521 acgaacccgg ccgtgaagag c
//
LOCUS       DAOPPA010000470         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_44709_length_2531_cov_3.906300, whole genome shotgun sequence.
ACCESSION   DAOPPA010000470 DAOPPA010000000
VERSION     DAOPPA010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44709_length_2531_cov_3.906300"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PKH54_12120"
     CDS             <1..1222
                     /locus_tag="PKH54_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOD00685.1"
                     /translation="EDREEFAALCHRHDIRIPQSRTATNRDQAMAAAMELGFPLMLRP
                     SFVLGGEGMAIAWNMEDLETRLDNAILVTSEKPLLLDRFLEDATEIDLDVISDGTDIH
                     TCGFLEHIEPAGIHSGDSIQVFPPQTIDEPDQARMLEIARTLAVELGAIGLMNLQFAR
                     CQGLIYLLEVNPRASRTVPFLHKASGSPWARHAARVMTGRKLSQLGLPMPDPRGRVWV
                     KAPVFPFSRFGIDPVLGPEMRSTGEVMGSGPDLGHALVKALRAAGCVLHRSGAIFVSL
                     NRRDRTRPLAIELARMLRGMEAEIYATEGTARFLMENGVKSQILSKLGEGGRDAVTIL
                     REAGISLILNTPAGGRGRTDAASIRVAASANGVACVTTLQGSIAALRGVMALRESALD
                     IRALQDDDGQSRAM"
     gene            complement(1247..2302)
                     /locus_tag="PKH54_12125"
     CDS             complement(1247..2302)
                     /locus_tag="PKH54_12125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOD00686.1"
                     /translation="MKSSSGQISMVQPEFAGIRVTMAACMIFFAAMTIFKMVLTDWLS
                     GLFYLAALLIMGIFFFQVLRTERYSRGVWFLESIFFLVWMAAIRVAGGGTQPGIDAGA
                     VVVMAFAIVMGSGILRLVVILLSLALPILLRVLETRWSTELYVARPFAVTRLVTGATV
                     LSMAVVLGLLMDRVVAAYFRQRELAQARLVTLSRMLVTDGMTGLANQTSLFERLSDEV
                     GRARRNRTTMSVVMIDIDDFKAVNDVHGHLAGDAVIVGVAGVLASIVSPPEFAGRRGG
                     DEFVVVLPDSGIDQAMEVAAKIMEGVSTLRGARRITVSIGVAAWGGQDAADLMSIVDA
                     RLRRAKELGKDRIVSSD"
     gene            complement(2299..>2531)
                     /locus_tag="PKH54_12130"
     CDS             complement(2299..>2531)
                     /locus_tag="PKH54_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00687.1"
                     /translation="LAVAERMLGCIGGVPVGQDGRVLACSIGVFQFDGRTPAESIHAA
                     DELMYRAKKDGGARVVAGTPSLPPGRLEVQTA"
BASE COUNT          543 a          764 c          800 g          424 t
ORIGIN      
        1 agaggatcga gaggaattcg cggcgctgtg ccacagacac gacatccgaa tcccgcagag
       61 caggacggcg accaatcgcg accaggcgat ggccgcggcg atggaacttg gatttccact
      121 gatgttgcgt ccgtcgttcg tcctgggcgg cgaagggatg gcaatcgcct ggaatatgga
      181 ggatctggaa acacgactgg acaacgcgat ccttgtgaca agcgagaagc cccttcttct
      241 tgacaggttc ctggaggacg cgaccgaaat cgatctggac gtgatttccg atggaaccga
      301 catacacact tgcggtttcc tggagcatat cgagccggca ggcatacatt ccggagactc
      361 gatccaggtc ttcccgccac agaccatcga tgagcccgac caggcccgga tgcttgaaat
      421 cgcccggacg ctggccgtcg aactgggcgc gatcggactg atgaacctgc agttcgcgcg
      481 gtgccaggga ttgatttacc tgctcgaggt caatccaagg gcaagccgga cggttccgtt
      541 ccttcacaag gcatctggtt cgccctgggc caggcacgcg gcccgcgtca tgaccggacg
      601 gaagctgtcg cagcttgggc ttccaatgcc cgacccgcgt gggcgggtat gggtgaaggc
      661 tcctgtgttc ccgttctcca ggttcggaat cgacccggtt ctggggccgg aaatgcgatc
      721 gacgggcgaa gtcatgggat cggggccgga tctgggacat gcactggtta aggcccttcg
      781 agccgccgga tgcgtcctgc acaggtcggg tgccatcttc gtaagcctga acagaaggga
      841 caggaccagg ccgctggcga tcgaactggc caggatgctt cgtggcatgg aagcggaaat
      901 ctatgcgacg gaaggtaccg caagattcct gatggaaaac ggcgtgaaat cccagattct
      961 ttcgaaactt ggcgagggcg gccgggacgc cgtgacaatc cttcgggaag ccgggatctc
     1021 gcttatcctg aacaccccgg ccggcggaag gggacgtacc gacgcggcat ccataagggt
     1081 tgccgcaagc gcgaacggcg ttgcgtgcgt cacgacgctt cagggaagca tcgcggccct
     1141 gcggggggtc atggcgctgc gggaatcggc cctggacatc agggcgctgc aggatgacga
     1201 cggtcaatcc cgcgcgatgt gaatcagtcc gaagactcgg cgcggatcag tccgaggata
     1261 cgatgcgatc cttgcccagt tcctttgccc tgcgaagcct tgcatcgaca atcgacatca
     1321 ggtcggcggc atcctggccg ccccaggcgg cgactccgat actcacggta attcgcctgg
     1381 cgccacgcaa ggtcgataca ccttccatta tttttgcggc gacttccatg gcctggtcga
     1441 tgcccgaatc gggcagcacg acgacaaatt catcgccgcc ccgacgaccg gcaaactcgg
     1501 gcggactgac gatgcttgcc aaaacgccgg caaccccgac gatgacggcg tcgccggcca
     1561 ggtggccatg aacgtcgttg actgccttga agtcgtcgat gtcgatcatc acgacggaca
     1621 tggtcgtcct gttgcgcctg gcgcgaccga cttcatcgct caaacgttca aaaagactgg
     1681 tctggttggc cagcccggtc atcccgtcgg taacaagcat ccgggaaagc gttacaaggc
     1741 gcgcctgggc gagttccctc tgccggaagt acgcggcgac gacgcgatcc atcagcagcc
     1801 ccaggacgac ggccatggaa agcacggttg cgccggtgac aagcctggtc acggcgaacg
     1861 ggcgggcgac gtacaactcg gttgaccacc tggtttccag gacgcgcaga aggattggaa
     1921 gcgcaaggga aagcaggatg acgaccaggc gaaggatgcc ggatcccatt acaatcgcga
     1981 aggccatgac gacgacggcg ccggcgtcga tgccgggctg agtcccgccg ccggcaacgc
     2041 ggattgccgc catccagacc aggaagaaga tactttccag gaaccatacg ccgcgtgaat
     2101 aacgctcggt tcgaaggacc tgaaagaaga atatccccat gatcagaaga gcggccaggt
     2161 agaacaaccc ggacagccag tccgtcagaa ccatcttgaa gatggtcatc gcggcaaaaa
     2221 agatcatgca ggcggccatc gtgacgcgaa ttccggcgaa ttccggctgc accatcgata
     2281 tctggccgct ggaagacttc atgccgtctg aacctccagg cggccggggg gaagtgacgg
     2341 agttccggcg acgacgcggg cgccaccatc cttcttggcg cggtacatca gttcatcggc
     2401 cgcatgaatc gactcggccg gcgtgcgacc gtcaaactgg aacaccccga tactgcacgc
     2461 aaggacacgc ccgtcctggc caacggggac gccgccgatg cacccgagca tcctctcggc
     2521 aaccgccagg g
//
LOCUS       DAOPPA010000471         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_45011_length_2518_cov_3.820544, whole genome shotgun sequence.
ACCESSION   DAOPPA010000471 DAOPPA010000000
VERSION     DAOPPA010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45011_length_2518_cov_3.820544"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="PKH54_12135"
     CDS             <1..1540
                     /locus_tag="PKH54_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00688.1"
                     /translation="IPLELTEENVAANVTDPVIDTIHDAVEARLTVLLDAIPADSTAT
                     TFVSTDPFKTEMATAIADARAEVRAKVAQKVAGMEEGMRESIVSSAEDQAGKVIQSAV
                     NGAAKAIKALPADVLADPESQAALMVKIEYKTRAFDDVPPIIVESVAEVRKQQLMAYW
                     DTIKAQWGMADMDLFATIDLNMNLERKYMMRYWNRIGAAVQQADPNAILFIGDPLAIN
                     SLFGGEAMSQMNNSPYKPENIKNFVHSYHWYPGSIYPLGLFQVPPQKFSAIEWRTKDF
                     LTPLSGKYTRTANTYGNVPVVFTEFGTYFNFNWNLKDRESILAHVAEHGYDVSAAILN
                     NEYETFEKLMSHRMVWCMSGDNDYQLGDLWNHEDFSVYGPDGEPRAHEAWMRPTVHMS
                     AGEPIEMRFFSPLHVFDPDKGESADQQLGEFHYACEGKESDLPTEIYVPEFQYPDGFY
                     VWLSDGWATFDPDLQTLYHYPTNDEPGVTHEVTIRRPAEGRENKGWAYFFNFDGQKVV
                     GDRQ"
     gene            1553..>2518
                     /locus_tag="PKH54_12140"
     CDS             1553..>2518
                     /locus_tag="PKH54_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00689.1"
                     /translation="MKRIIFPIVVFAVAVLAGHAAISQEAGQESWPEAAPSQDESIES
                     DGSGESAGGEANSDAESAAGGDSEGGAEKSDAAPSDAAPSDADKAFGRDPRPKIDAFY
                     SKHVDIGFQLATMFQYSNDSDFDRSKPLDDPYDRSTGLFGTFLKPQLTVKLNKVFRFY
                     WELELGLDLWSRNSPDATLGADGKAKEPESFGIRQRELYGEVLYRQLAVRLGYQHIED
                     VSGLFLNHWFGAATFRYGPERSSHVRLFGGQMPDQTYEGWDLTENSLKNDVYIAGIDG
                     QWVFNRWAKIQAGAYYLGDLSTVGQYRHVGVLSAGFALEKKCWDAR"
BASE COUNT          530 a          739 c          764 g          485 t
ORIGIN      
        1 gattccgctg gaattgaccg aagagaatgt ggccgccaac gtcaccgacc ccgtgatcga
       61 cacaatccac gacgcggtcg aggcgcggct tacggtactg ctggacgcga tccccgccga
      121 ttccaccgcc accacttttg tttcgaccga tccgttcaag accgagatgg ccaccgccat
      181 cgccgacgcc agggccgagg tgcgcgcgaa ggtcgcccag aaggtggccg gcatggaaga
      241 aggcatgcgc gagtcgatcg tttcatccgc cgaggatcag gccggaaagg tcatccagtc
      301 cgcagtcaac ggcgcggcga aggcgatcaa ggcactgccg gccgacgtcc tggccgatcc
      361 ggaatcgcag gcggccctga tggtgaagat cgaatacaag acgcgcgcct ttgacgacgt
      421 gcccccgatt atcgtcgagt ccgtcgccga ggttcgcaag cagcagttga tggcctactg
      481 ggacacgatc aaggcccagt ggggaatggc cgacatggat ctgttcgcca cgatcgacct
      541 gaacatgaac ctggaacgca agtacatgat gcgttactgg aaccgcatcg gcgcggcggt
      601 ccagcaggcc gacccgaacg cgatcctgtt catcggcgac ccgcttgcga ttaacagcct
      661 gtttggcggc gaggcgatgt cccagatgaa caattcgcct tacaaacccg aaaacatcaa
      721 gaatttcgtg cattcgtacc actggtatcc gggcagtatc tatccgcttg gtctgttcca
      781 ggtgccgccg cagaagtttt cggccatcga gtggcgcaca aaggacttcc tgacgccgct
      841 gtcgggcaag tacacccgca cggccaacac gtacggcaac gttccggtgg tgttcacgga
      901 attcgggacg tacttcaatt tcaactggaa cctgaaggat cgcgaatcga tcctggctca
      961 cgttgccgag catggctatg acgtgtccgc ggcaattttg aataacgaat acgagacgtt
     1021 tgaaaaactg atgtcgcaca ggatggtatg gtgcatgtcg ggcgataacg actaccagct
     1081 tggcgacctt tggaaccacg aggacttcag cgtctatggt ccggacggag agccccgcgc
     1141 ccacgaggca tggatgaggc cgaccgtcca catgtcggcc ggtgaaccga tcgagatgcg
     1201 cttcttctcg ccgctgcacg tctttgaccc ggacaagggc gaaagcgccg accagcagct
     1261 tggtgaattc cattacgcct gcgagggcaa ggaatcggac cttccgaccg agatttacgt
     1321 gcccgaattc cagtacccgg acggcttcta tgtctggctt tcggacgggt gggcgacgtt
     1381 cgaccccgat ttgcagacgc tttaccacta tccgacaaat gacgagccgg gcgtgacgca
     1441 tgaagtcacg atcaggcgcc cggccgaagg tcgggaaaac aagggatggg cgtatttctt
     1501 caacttcgat ggccagaagg tcgtcgggga caggcaatag gagggcacgc agatgaaacg
     1561 catcattttc ccgattgtcg ttttcgccgt ggccgtcctt gccgggcacg ccgcaatttc
     1621 ccaggaagct ggccaggaat cctggcccga agccgccccg agccaggacg agtccatcga
     1681 atccgacggc tctggcgaat cggccggcgg tgaagccaat tccgatgccg aaagcgccgc
     1741 cggtggtgac tccgagggtg gcgctgaaaa gtccgatgcc gccccgtctg acgccgcccc
     1801 gtccgacgcc gacaaggcgt tcggtcgcga tccccgtccg aagatagacg ccttttattc
     1861 caagcacgtc gatatcggat tccagcttgc gacgatgttc cagtactcaa acgacagcga
     1921 tttcgatcgc agcaagcccc ttgatgaccc gtacgaccgt tcgaccggac tgttcggaac
     1981 cttcctgaag ccgcagttga cggtcaagct gaacaaggtc ttccgctttt actgggaact
     2041 tgagcttggc ctggatctgt ggagtcgtaa ttcgccggat gcgacgcttg gcgctgacgg
     2101 caaggcaaag gaaccggaat cgttcggcat ccgtcagcgg gagctttacg gcgaggttct
     2161 ttacaggcaa ctggcggttc ggcttggata ccagcatatc gaggacgtat ccggcctgtt
     2221 cctgaaccat tggttcggcg cggccacctt ccgttatggc ccggaacgct cgagtcatgt
     2281 gcgtctgttt ggcggccaga tgccggatca gacgtatgag ggctgggatc tgaccgaaaa
     2341 cagcctgaag aacgacgtgt acatcgccgg tatcgacggg cagtgggttt tcaatcgctg
     2401 ggccaagatt caggccggcg cctattacct tggggatctt tccacggtcg gacagtaccg
     2461 ccatgtcggg gtgctctcgg cgggattcgc ccttgaaaag aagtgctggg acgccagg
//
LOCUS       DAOPPA010000472         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_45121_length_2513_cov_4.203011, whole genome shotgun sequence.
ACCESSION   DAOPPA010000472 DAOPPA010000000
VERSION     DAOPPA010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45121_length_2513_cov_4.203011"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2207
                     /locus_tag="PKH54_12145"
     CDS             <1..2207
                     /locus_tag="PKH54_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HOD00690.1"
                     /translation="FRSENTSGLGIRALAAAIEAEGFEVVGVTSYGDLASFAQQQSRA
                     SAFILSMDDDELANEPEQTIDELRAFVEEIRFRNTDIPLFLYGETRTARHIPNDVLKE
                     LHGFIHMFEDTPEFIARYVVREAKSYVDSLPPPFFRALTHYAADSSYSWHCPGHSGGV
                     AFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAAAERNAARIFNCDHLL
                     FVTNGTSTSNKIVWNSTVAPGDIVVVDRNCHKSVLHAIMLTGALPVFLMPTRNNLGII
                     GPIPRSEFEWENIKKKMMANPFTAEILKNDPETKPRVLTITQSTYDGVIYNDEEIKEM
                     LDGHIDTLHFDEAWLPHATFHKFYKGFHAIGKSSRRCRESLVFSTQSTHKLLAGLSQA
                     SQILCQDSETRQLDRDVFNEAFLMHTSTSPQYAIIASCDVAAAMMEQPGGKSLVEESI
                     KEALDFRRAMRKVEAEWGDDWWFKVWGPDELAEEGIGDPSDWMLKNDDHWHGFGKLAT
                     GFNMLDPIKGTVVTPGLDVDGDFADWGIPAIIVTKYLAEHGVIVEKTGLYSFFIMFTI
                     GITKGRWNTLMSELQQFKDLYDRNQPMWKVMPEFVAKYPVYERMGLRNLCDEIHDVYK
                     ANDVAKLTTEMYLSDMIPAMKPADAFAKMAHREIERVPIDDLEGRVTAVLLTPYPPGI
                     PLLIPGERFNAKIINYLKFARSFNRKFPGFETDNHGLVKKDGDYFIDCVVFD"
BASE COUNT          533 a          748 c          758 g          474 t
ORIGIN      
        1 acttccgttc ggagaacacg tccgggctcg gcatccgcgc ccttgccgcc gccatcgagg
       61 ccgagggctt cgaggtcgtg ggcgtgacca gctatggcga ccttgcgtcg tttgcccagc
      121 agcaaagccg cgcatccgcg ttcatccttt cgatggacga cgatgaactg gccaacgaac
      181 ctgaacagac cattgacgaa cttcgcgcat ttgttgaaga aatccggttc cgcaataccg
      241 acatcccgct gttcctttac ggggaaacca ggacggcccg ccacatcccg aacgacgtgc
      301 tgaaggaact gcacggcttc atccacatgt tcgaggacac gccggaattc atcgcccgat
      361 acgtcgtgcg cgaggccaag tcgtacgtcg attcgctgcc gccgccgttc ttccgggcgc
      421 tgacccacta cgcggccgac agcagctatt cgtggcactg cccgggccat tccggagggg
      481 tcgcattcct taagtccccg gtcggtcaga tgtttcacca gtttttcggc gaaaacatgc
      541 tgcgcgccga cgtgtgcaac gcggtcgagg aacttggcca gcttctggat cacaccggcc
      601 cggtcgccgc ggcggaacgc aatgccgcgc gcatattcaa ctgcgatcac cttctgttcg
      661 tcacgaacgg gacatcgacg tccaacaaga ttgtgtggaa ctcgaccgtc gcccccgggg
      721 acatcgtggt cgtcgatcgc aactgccaca agtcggtgct tcacgcaatc atgctgaccg
      781 gcgcgctgcc ggtcttcctg atgccgacac gcaacaacct gggaatcatc gggccgatcc
      841 cgcgttccga attcgaatgg gaaaacatca agaagaagat gatggccaac ccgttcacgg
      901 ccgagatcct gaagaacgac ccggaaacga agccgcgagt cctgacgatc acgcaatcga
      961 catacgacgg cgtcatttac aacgacgaag aaatcaagga aatgctggac gggcacatcg
     1021 acaccctgca tttcgacgaa gcatggctgc cgcacgcgac gttccacaag ttttacaagg
     1081 gttttcacgc gatcggaaaa agcagcaggc gatgccgcga gtcgctggtg ttttccacgc
     1141 aatcgacgca caaactgctg gccggcctgt cccaggcttc ccagatcctg tgccaggaca
     1201 gtgagaccag gcaacttgac cgcgacgttt tcaatgaggc gttcctgatg cacacatcga
     1261 cgtcgccgca gtacgcgatc atcgcgtcgt gcgacgttgc ggcggccatg atggagcagc
     1321 ccggcggcaa gtcgctggtc gaggaatcga tcaaggaggc gctggacttc cgtcgcgcca
     1381 tgcgcaaggt cgaggccgag tggggcgacg actggtggtt caaggtctgg ggtccggacg
     1441 agcttgccga ggaaggcatc ggggatccgt cggactggat gctgaagaac gacgatcact
     1501 ggcatggttt cggcaaactg gcgaccggct tcaacatgct ggacccgatc aagggaacgg
     1561 tcgtgacccc cgggctggac gtggacggcg acttcgccga ctggggaatc ccggcgatta
     1621 tcgtgacgaa gtacctggcc gaacacggtg tcatcgttga aaagacgggc ctgtattcgt
     1681 tcttcatcat gttcacgatc ggcatcacca agggccgctg gaacacgctg atgtccgaac
     1741 tgcagcagtt caaggacctt tacgaccgca accagccgat gtggaaggtc atgccggaat
     1801 tcgtggccaa gtacccggtt tacgaaagga tggggttgcg caacctttgc gacgagatcc
     1861 acgacgtcta taaggcgaac gacgtggcca agctgaccac cgagatgtac ctttcggaca
     1921 tgatcccggc catgaagccg gccgacgcgt tcgcgaagat ggcgcaccgc gagatcgagc
     1981 gtgtgccgat cgacgacctg gaaggtcgcg tcaccgcggt tctgctgacg ccgtacccgc
     2041 cgggaatccc gcttctgatc cctggcgagc ggttcaacgc caagatcatc aactacctga
     2101 agttcgcccg ttctttcaac cggaagttcc cgggatttga aacggacaac cacggtctgg
     2161 tcaagaagga cggggactac ttcatcgact gcgtcgtatt cgactgaaag cgttggttcg
     2221 cagcgggcga attttcattc gcccgtacgg atcgctgggc ggatgcatct tgctgaaccg
     2281 cagcgggcgg atgtaatccg cccgtacaag gagaaaaggc gggcgagttt tcattcgccc
     2341 ttacggggag agcggcgggc ggcgcggcgg gcgaattttc acgcgggcgg gcgggtcggg
     2401 atcgacagtt cgccggcaaa attggtctgc atcacggtgg cgatatcctt gtactggtaa
     2461 tgtttcaagg cccacatcag cttgaccgcc aacatttcca ggctctggtc gtg
//
LOCUS       DAOPPA010000473         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_45629_length_2491_cov_4.600985, whole genome shotgun sequence.
ACCESSION   DAOPPA010000473 DAOPPA010000000
VERSION     DAOPPA010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45629_length_2491_cov_4.600985"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKH54_12150"
     CDS             complement(<1..300)
                     /locus_tag="PKH54_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD00691.1"
                     /translation="MSAGKPENKYDVMVVGGGIAGQEAALNLASMGRKVLLVEKALSI
                     GGKMIQLSKVFPTLDCAACITTPKMSETARNTSITLSLNTTIDSIQKVADGFDVQL"
     gene            complement(297..707)
                     /locus_tag="PKH54_12155"
     CDS             complement(297..707)
                     /locus_tag="PKH54_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD00692.1"
                     /translation="MQKLTSSPKILVFSTEKISDPGIDQAGLRKIHYSPSVYVISVPC
                     SAGIKPKWIMHAFAQGFDGVFIAADGHECSYTPKCSELTNELIEHSQKLMQAKGIDPK
                     RIRMAAICSVCAEQFASHMANFTRILVELNGKAE"
     gene            complement(767..1018)
                     /locus_tag="PKH54_12160"
     CDS             complement(767..1018)
                     /locus_tag="PKH54_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOD00693.1"
                     /translation="MTTDELKGLKVASLVDARGTACPGPLLAAKKAMGDIESGEILEV
                     YSSDEGTKVDIPKWCQKMGHDYLGVVEESSYVRMFLKKS"
     gene            complement(1034..1552)
                     /locus_tag="PKH54_12165"
     CDS             complement(1034..1552)
                     /locus_tag="PKH54_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOD00694.1"
                     /translation="MAENTNTTVSTGPETEQISMVVFSGDLDKLLAAMIIATGAAAYD
                     MKVNLFFTFWATAALRAPSKKAAGKDFMSKMFGWMLPKGAEKVALSKMNMGGMGTAMI
                     KGIMKKHKVASLPELLKLAGELGVNINVCEMSMNLMGFKKEELIDYPNMKICGVATFL
                     SDASESKIQLFI"
     gene            1712..2047
                     /locus_tag="PKH54_12170"
     CDS             1712..2047
                     /locus_tag="PKH54_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOD00695.1"
                     /translation="MATSREFMEFVLEQISSMPTVRARKMFGEYALYCDDKVVGLVCD
                     EQVFIKITPEGRALVGDRYEEGLPYPGAKPWMLVDGDEIEESDRMCSLIRATADALPS
                     PAPKRPRTR"
     gene            complement(2113..>2491)
                     /locus_tag="PKH54_12175"
     CDS             complement(2113..>2491)
                     /locus_tag="PKH54_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00696.1"
                     /translation="CVAVEVEPVPEMVEEGDEATVADDAGDAADVAIGDEGGEDAVVA
                     LDSVADSVVDAVADAGRDLAQPDTAAGDAPDQIEGDTGNVEDTVTGGGKGGCSAGSSP
                     LSMMPALLLLALAAFVLRLRRRA"
BASE COUNT          508 a          761 c          686 g          536 t
ORIGIN      
        1 aagctgcaca tcaaaaccgt ccgccacctt ctgaatcgaa tcgatcgtgg tgttcaggga
       61 cagcgttatg ctggtgttgc gggccgtctc ggacatcttc ggtgtggtga tgcacgccgc
      121 gcagtccaga gtcgggaaca ccttgctcaa ctggatcatc ttgccgccga tggacagcgc
      181 cttttcgacc agcaggacct tgcgccccat ggacgccagg ttcagcgcgg cctcctgtcc
      241 ggcaatgccg ccgccgacga ccatcacgtc gtacttgttt tccggttttc ccgcgctcat
      301 tctgccttcc cgttaagttc gaccagaatc ctggtgaaat tggccatgtg gcttgcaaac
      361 tgctcggcgc aaaccgaaca gattgccgcc atcctgatgc gcttcgggtc gattcccttc
      421 gcctgcatca gcttctggga atgttcaatc agctcgttgg taagttcgct gcacttcggg
      481 gtgtagctgc actcgtgacc gtcggccgcg atgaacacgc cgtcaaaacc ctgcgcgaac
      541 gcgtgcatta tccacttcgg cttgataccg gccgaacacg gcacagaaat gacatatacc
      601 gatggcgagt agtggatctt gcgcaatccg gcctggtcga tgccgggatc cgagatcttt
      661 tccgtggaaa agaccagtat ctttggagag ctggttaact tctgcatgcg cgcgttcacc
      721 tgaaaagaga atggccccga catgaacgca aggcgacatg ccgttatcag gacttcttca
      781 ggaacatcct gacgtagctg gactcctcga cgacaccgag gtaatcgtgg cccatcttct
      841 gacaccattt gggaatatcg acctttgtgc cttcatcgga cgaatacact tccaggattt
      901 ccccggattc gatgtcaccc atggccttct ttgccgcaag cagcgggccg gggcaggcgg
      961 ttccacgggc atcgacaagg ctggcaacct taagtccctt cagttcatcg gtggtcatgg
     1021 cgcatctcct taatcagatg aaaagctgga tcttgctttc ggacgcgtcg ctcaggaagg
     1081 tggcgacgcc gcagatcttc atgttcgggt aatcgatcag ttcttccttc ttgaagccca
     1141 tcaggttcat ggacatctcg cacacattga tgttgacgcc gagctcgccc gcaagcttaa
     1201 gcaactcggg aagtgaagcc accttgtgct tcttcattat ccccttgatc atggcggtac
     1261 ccatcccgcc catgttcatc ttcgaaagcg ccaccttttc ggcgcccttc ggcagcatcc
     1321 atccgaacat cttcgacatg aagtccttgc ccgcggcctt cttcgacggc gcccgaagcg
     1381 ccgccgtggc ccagaaggtg aagaacaaat tcaccttcat gtcgtatgcc gccgcgccgg
     1441 tcgcaatgat catcgcggca agcagcttgt ccaggtcgcc ggaaaagacg accatggaaa
     1501 tctgttctgt ctcggggcca gttgaaacgg ttgtgtttgt gttttcagcc atagtggaca
     1561 aaacttatca atttcatatg agatgtcaac aaattcattc aatccccctg ggatttaagg
     1621 gttttcaccg aaaatcggtt ctttccgacc ttgtcttgga ctttggatgc accgctgatt
     1681 aacattcaag ggtccccgaa ctggtgaagt gatggcgaca agccgggaat tcatggaatt
     1741 cgtgctcgaa caaatctcct cgatgccgac agtgcgcgcc aggaagatgt tcggcgaata
     1801 cgcgttgtac tgcgatgaca aggtcgtcgg actggtgtgc gacgaacagg tcttcatcaa
     1861 gataacgccg gaaggcaggg ctctggtcgg ggatcggtac gaagagggtc ttccctaccc
     1921 cggcgcgaag ccctggatgc tggtcgatgg ggacgaaatc gaagagtccg accgcatgtg
     1981 ttctctgatc agggccaccg ccgacgcact gccatcgccc gccccgaaga ggcctcgcac
     2041 aagataacgg acagtgtcag tccggaacag aaagatcgcg gctggacctg tgaacggttg
     2101 ctggatcaga agtcacgccc ggcggcgcag gcgcaggaca aacgcggcaa gggcaagcag
     2161 cagcagagcc ggcatcatgg acagcgggct cgagccggcc gagcagccgc ccttgccacc
     2221 tccggtgacg gtgtcttcga cgtttccggt gtcgccctcg atctggtcgg gggcgtcgcc
     2281 agcggcggtg tccggctggg caagatcccg gccggcatcg gcaaccgcgt ccacaaccga
     2341 atcggcaacc gaatccagcg ccaccacggc gtcttcgccg ccctcgtcgc caatcgcaac
     2401 gtccgccgcg tccccggcat cgtccgcaac cgttgcctcg tcgccctctt ccaccatctc
     2461 gggcaccggt tcgacctcga ccgccacgca c
//
LOCUS       DAOPPA010000474         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_45853_length_2482_cov_4.295426, whole genome shotgun sequence.
ACCESSION   DAOPPA010000474 DAOPPA010000000
VERSION     DAOPPA010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45853_length_2482_cov_4.295426"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKH54_12180"
     CDS             <1..547
                     /locus_tag="PKH54_12180"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD00697.1"
                     /translation="IRIQTRPERRDIPAQRINLDICAVPGRDPQPQMIRDLYGIATGA
                     PGLVLQAESRGEIFVDKLIAFALRRGRIKHRDIWDIGWMVQQSVKPRMDLLTLKLDDH
                     GSCVDAFLPAFDERVASLSNPEMASGFRDEMQRFLPSAFVARTVDDPHFWTWMIGVMS
                     EQRVGVRNTLTGAADPAAFPM"
     gene            complement(577..2343)
                     /locus_tag="PKH54_12185"
     CDS             complement(577..2343)
                     /locus_tag="PKH54_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00698.1"
                     /translation="MVTHLTTNRIKALVAGGKPFADAVAQAEGELLAQLDITGPDYQP
                     GVAATGMNVAGGDNDDNAYLLAVSSVLIKVAAARQGAVESNIQEVLNRIAQDLIGGTI
                     NEGLKTEITTALLAVDPDSIARRLQARLDTLGSDATVPDMNRVLDQDRDGKVNAIDNC
                     PLVVNDNQLDEDKDGVGDACDNCPATACPRPNSCLPDSAGIHSQDICYLPCVEDGMNY
                     LCEGGTCASVPWRDNEGTLQDIVACTQFCDPLASTPCGQDQYCGHIDTFLGIRVTGDR
                     HFACAPDALQPTAAAVIYCFVEVTGQQQLRQCLEDAQCDRTPTGLVMRDGCPEGQVCA
                     DILGPTWGRFCSAACDPAGTNTCLADLACRGPVEGETFGLCDPLPRGTEGMPCDPDGD
                     CDGDLTCGYGNFQGIELCLIPDTNCCYQGTPAPGLYEPCGAGNVCAGTLQCSTFSGQC
                     PLGFPNCCIDTTKVGTLGHACDPVGQCGAGLSCTDSSACDPLKYCCLQESGGEGAPCE
                     APDYTCEEGFGCVSGGTAGINCMGNPYCCTAGAGDNLGLCVMGAMGAFSCNDPDNYFC
                     GQPEPPDRCNSQQVGMCCVPKK"
BASE COUNT          460 a          737 c          809 g          476 t
ORIGIN      
        1 aatcaggata caaacgcggc cggagcgtcg cgacattccg gcccagcgaa tcaaccttga
       61 tatatgcgcg gttccgggcc gtgaccctca gccacagatg atccgggacc tttatggcat
      121 tgcgaccggc gccccgggac tcgtgctcca ggcagagagc aggggcgaga tattcgtgga
      181 caagctgatc gcgttcgcgc ttcgtcgcgg gcggatcaag caccgcgaca tatgggacat
      241 cggctggatg gtgcagcagt ctgtcaaacc acggatggat ctgctgacgt tgaagctgga
      301 cgatcacggt tcatgtgttg atgctttcct gcctgcgttc gatgaacggg tcgcgagtct
      361 ttcaaatcca gagatggcaa gcggcttccg ggacgagatg cagcgtttcc ttccgtccgc
      421 ctttgtcgcc cggacagttg atgatccgca tttctggacc tggatgatcg gcgtgatgtc
      481 cgaacagcgc gtcggcgtca ggaacacgct taccggcgct gccgatccag cagcgttccc
      541 gatgtgaatc gatatcgtgc ctgattcagt cggaggctac ttcttcggga cgcagcacat
      601 gcctacttgc tggctgttgc aacggtcggg cggctctggc tgtccgcaga agtagttgtc
      661 ggggtcgttg cacgagaagg cacccatcgc ccccatgaca cacaggccga gattgtcgcc
      721 ggcgccagcc gtgcagcaat acggattgcc catgcagttg atgcccgcgg ttccgcccga
      781 aacgcagccg aaaccttcct cacaggtgta atcgggcgct tcgcaggggg ctccctcacc
      841 gccggactcc tgaaggcagc agtatttcag cgggtcgcac gcggaactgt cggtgcagga
      901 aaggccggcg ccgcactgac caaccggatc gcaggcatgc ccaagcgtac cgaccttcgt
      961 cgtatcgata cagcagttgg gaaatcccag gggacactgg ccgctgaagg tggagcactg
     1021 aagcgtgccg gcgcagacgt tacccgcacc acacggctcg taaaggcctg gcgccggggt
     1081 tccctggtaa cagcagttgg tgtcggggat caggcacagt tcgattccct ggaagttccc
     1141 gtaaccgcag gtaaggtcgc catcacaatc tccgtcgggg tcgcatggca tgccctcggt
     1201 gccgcgcggc agggggtcgc acaggccgaa cgtttcaccc tcgacggggc cacggcaggc
     1261 caggtcggca agacaggtgt ttgttccagc cgggtcgcat gccgcagagc agaagcgccc
     1321 ccatgtcggg cccaggatgt cggcgcatac ctgaccctcg gggcatccat cgcgcatgac
     1381 aagaccggtt ggggttctgt cgcactgggc atcctcgagg cactggcgca gttgctgctg
     1441 ccccgtgacc tcgacaaaac agtagatgac ggcggcggcc gttggctgca acgcatccgg
     1501 ggcacaggcg aaatgcctgt ctcccgtaac gcggataccc aggaatgtgt cgatgtgccc
     1561 gcagtactga tcctggccgc acggggtgga ggccagcggg tcgcagaact gggtgcatgc
     1621 aacgatatcc tgaagagtcc cctcattgtc gcgccaggga acggatgcgc atgtgccgcc
     1681 ttcgcaaaga taattcatgc catcctcgac acaaggcagg taacagatgt cctggctgtg
     1741 gatgccggct gaatctggca gacatgagtt ggggcgtggg caggcggtgg cgggacagtt
     1801 gtcgcaggcg tccccgacgc cgtccttgtc ttcgtcgagc tggttatcgt tgacgaccag
     1861 cgggcagttg tcgattgcgt tgaccttgcc gtcacgatcc tgatcaagga cgcggttcat
     1921 gtcgggaacc gtggcgtccg aaccgagggt atcgaggcgg gcctgcaggc ggcgggcaat
     1981 cgagtccggg tcaacggcaa gcagcgcggt ggtgatttcc gtcttcagtc cttcattaat
     2041 cgtgccgcca atcagatcct gggcgatgcg gttcaggact tcctggatgt tcgattcaac
     2101 ggcgccctga cgggcagcgg cgaccttgat gagaaccgaa ctgacggcaa gcaggtaagc
     2161 gttgtcgtcg ttgtcgccgc cggccacgtt catgccggtc gctgcgacgc ccggctggta
     2221 atccggcccg gtgatgtcaa gctgggccag aagttcaccc tcggcctggg caactgcatc
     2281 cgcaaacggc tttccgccgg cgaccagtgc tttgatccga ttggttgtca ggtgcgtgac
     2341 catgttgatg aaggcggtct gctggccctg gccggcggga acgtaaaagg cattcagtgc
     2401 cagggttgac gccgacagca gtccggtaac ctcgttgtag tagtagccgc tgccctgcag
     2461 cgcgaccggg ccggtcgcat cg
//
LOCUS       DAOPPA010000475         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_46018_length_2476_cov_4.806278, whole genome shotgun sequence.
ACCESSION   DAOPPA010000475 DAOPPA010000000
VERSION     DAOPPA010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46018_length_2476_cov_4.806278"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..131
                     /locus_tag="PKH54_12190"
     CDS             9..131
                     /locus_tag="PKH54_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOD00699.1"
                     /translation="MWVFQVSDFPATVTMDSTGSSLHESVLELSRERLAALVGP"
     gene            complement(140..613)
                     /locus_tag="PKH54_12195"
     CDS             complement(140..613)
                     /locus_tag="PKH54_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00700.1"
                     /translation="MTSKLKEHISEAINESKKQVQTIIEKGTVAAGVGREVAGKAIEK
                     AEDVAAKARAKAGETAAKGAVAVSVISEKVQKDNLVKMAESVRDEVRNTLFAETMSEI
                     ARLKDDTDELRRRMADMQKAYDRKIADLQDQIKAAGAEKPTARTARTKRAETVKE"
     gene            complement(735..2294)
                     /locus_tag="PKH54_12200"
     CDS             complement(735..2294)
                     /locus_tag="PKH54_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00701.1"
                     /translation="MRDVGAEGQVSGGKTIYEISPPKVLKVGATFDGKVVFKPDQAVE
                     VYTEIRAFSSDTDYPDGLLFHAMGNKTRPALEVTPESIDFGAAVIGTPVTRKVTLKSV
                     GRMDVNVYGVSPDAAALAAGFRVEFPDRQPTVQNPVTLAQTKTMEVNIVYDPSRASPV
                     NAIGRPQPESGQIEISGDMFTGKIFQRVTGVAVEGACTMPIVDFINEYRMDGDGCPVQ
                     PDYPADDKNTRICKTEMGWEVPTGTLVQLSGAQSFSPFGDVTQWQWSATQPAANTGFF
                     IPTENEVEPTFMIGAAPDLGAGENPYYEIFLNVIDNKGYEACAGKSIRITTRVEVKAD
                     ILLSWKPVNPFTPVPPFMGQDVDLHFTHFKAGDDGSEPDIDKDGLPDDWFDLPYDCFW
                     FNAEPEKYLWGEASPWINDRVVLRNDSDDGTEPEVLMMGLDCPAGRPFKVGAHYFDDH
                     GYGQADVTIQVYAKGQLLYTSTRRLSSHDMWYVGDLKCKTVGLDKTAEFIVNTTKPVT
                     PNYVNTSFGNP"
     gene            2332..2476
                     /locus_tag="PKH54_12205"
                     /pseudo
     CDS             2332..2476
                     /locus_tag="PKH54_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
BASE COUNT          431 a          760 c          721 g          564 t
ORIGIN      
        1 ccgaggcaat gtgggtcttc caggtctcgg actttcccgc gaccgtcacg atggattcga
       61 caggttcaag ccttcacgag agtgttcttg agctttcacg cgagcgcctg gcagcccttg
      121 tcgggccgtg accgcgggat cattccttca cggtttcggc tcgcttcgtc ctggccgtcc
      181 tggccgtcgg cttttccgca ccggccgcct tgatctgatc ctgcagatcg gcaatctttc
      241 tgtcgtacgc tttctgcatg tcggccatgc ggcggcgaag ttcgtccgta tcgtccttca
      301 gccttgcgat ctcggacatc gtttcagcaa acagcgtgtt ccggacttcg tcgcggaccg
      361 attcagccat cttgacaaga ttatccttct ggaccttctc ggaaatgacg gatacggcaa
      421 ccgcaccctt cgcggccgtt tcgccggcct tggcgcgggc ctttgccgcg acgtcctcgg
      481 ccttttcgat cgccttgccg gcgacctcac gacccacgcc ggccgcgacg gtgccctttt
      541 cgatgatggt ctgaacctgc ttcttcgact cgttgattgc ttcgctgatg tgttccttca
      601 gtttgctggt catagtgttt ctccaacttg actccaacac aaaataacgc gccgcgtcac
      661 aatgtcaagg ccacggaatg acaaaaattt ccacgcattc cggcggagcc gccgggccgc
      721 gtcaaatcaa tggatcacgg gttgccgaag ctggtgttga cgtagttcgg cgttaccggc
      781 tttgtcgtgt tcacgatgaa ctcggcagtc ttgtccaggc cgacagtctt gcatttcagg
      841 tcgcccacgt accacatgtc gtgggacgac aggcgtcgcg tcgaagtgta aagaagttgc
      901 cccttcgcat acacctggat cgtgacgtcg gcctgtccat agccgtggtc gtcgaagtag
      961 tgggccccga ccttgaacgg ccgtccggcc gggcagtcca ggcccatcat caggacttcc
     1021 ggttcggttc cgtcgtcgct gtcgtttcgc aggacgaccc ggtcgttgat ccaagggctg
     1081 gcctcgcccc aaaggtactt ttcgggttcc gcgttgaacc agaaacagtc gtagggaagg
     1141 tcaaaccagt cgtcgggcag gccgtccttg tcgatgtcgg gctcgcttcc gtcatcgccg
     1201 gccttgaagt gggtgaaatg aagatccacg tcctggccca tgaagggtgg cacgggcgtg
     1261 aacgggttga ccggcttcca ggaaagaagg atgtcggcct tcacctcgac cctggtcgtg
     1321 atccttatcg atttgccggc acaggcctcg tatcccttgt tgtcgattac gttaaggaaa
     1381 atttcgtagt aagggttctc gccggcgccg aggtccggcg cggcccctat catgaaggtc
     1441 ggctcgactt cgttttcggt cggaatgaag aagcccgtgt tcgcggccgg ctgggtcgcg
     1501 gaccattgcc actgcgtgac atccccgaac ggcgagaacg actgcgcgcc ggacagttgg
     1561 acaagggttc cggtcgggac ttcccagccc atttcagtct tgcagatcct ggtgtttttg
     1621 tcgtcggccg gatagtccgg ctgaaccgga catccgtcgc cgtccatcct gtattcgttg
     1681 atgaaatcga cgatcggcat ggtgcacgcg ccttcgacgg cgacaccggt gacgcgctgg
     1741 aaaatctttc cggtgaacat gtccccggaa atctcgatct gtccagactc cggctgcggc
     1801 ctgccgatgg cgttgaccgg gctggccctg gacgggtcat atacgatgtt cacttccatc
     1861 gtcttggtct gcgccagcgt gaccggattc tgcacggtcg gctgcctgtc ggggaactcg
     1921 acgcggaaac cggccgccag cgccgcggca tcgggggaaa cgccataaac gttcacgtcc
     1981 atgcggccga cggatttgag cgtgaccttt cttgttacag gggttccaat gaccgccgcg
     2041 ccgaaatcaa tggattccgg agtgacttcc agggccggcc ttgtcttgtt tcccatggcg
     2101 tggaacagca ggccgtccgg gtaatcggta tcggaagaaa atgcccggat ttcagtgtaa
     2161 acctcgacgg cctggtcagg cttgaaaaca accttcccgt caaatgtggc gccgaccttc
     2221 agcactttcg gcggggaaat ttcgtatatc gtctttccac ctgaaacctg gccctcggcg
     2281 ccgacgtcac gcacgattgc cgccggcccg accgtcgtgc tcaagcccga ggtcaagctc
     2341 gacaaggaga gcatcgccga ctacatccag cataaaaccg tgctcgtcac gggcggcggc
     2401 ggctccatcg gctcggagat ctgccgccag acggcgatgt tctatcccgc gcggctcatc
     2461 atcttcgata tctacg
//
LOCUS       DAOPPA010000476         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_46275_length_2467_cov_4.223466, whole genome shotgun sequence.
ACCESSION   DAOPPA010000476 DAOPPA010000000
VERSION     DAOPPA010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46275_length_2467_cov_4.223466"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(577..900)
                     /locus_tag="PKH54_12210"
     CDS             complement(577..900)
                     /locus_tag="PKH54_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00702.1"
                     /translation="MRKEHAMWSDEIDDITVEYEEDGQQVVKEEAKAIIARGAWPVLV
                     FKYREWSRAENAFGPPKYTIRKYRKHNGSYRIEAKFNIGKPEQAVEIAAILKEWATSD
                     LPVAD"
     gene            complement(900..1778)
                     /locus_tag="PKH54_12215"
     CDS             complement(900..1778)
                     /locus_tag="PKH54_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00703.1"
                     /translation="MKFDLAVIVLAIALLVPGCSDPGPKGASGAVDTAGRPVAETPAW
                     SLRYFMQTGFHAESNLTMVIRRADRTVEIVDSATMNLLADGEMSFVRSRTHKGAAEGV
                     SVESFSATRKDGSWFTAGSSGQWVRWDDAITQPSAMVDSFIAGEDSLLRIAHDCGRQG
                     KPDEEGRVPLTLLADRCRFETSVMPGSTKTFGGRVNSLSGYLVMSGNAPAGAAIRIEF
                     VSTHGGGETPVSLNYELRVSPGGIPGQIAAPKHFVESRRPRPVKMVESVLSGIVKDWG
                     PGAPEVLRKRPPGSGR"
     gene            complement(1775..>2467)
                     /locus_tag="PKH54_12220"
     CDS             complement(1775..>2467)
                     /locus_tag="PKH54_12220"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD00704.1"
                     /translation="GCVNVHGSILPSYRGAAPIQWAVINGESETGVTIMMMDEGCDTG
                     DILLIGRTPISATDTAATLFERLSQMGPPLLLQALGQLACGAAVRIAQDHSLATVAPM
                     IDKSVGLIDWTSSSRSIANLVRGVDPWPGAFTFTPDGTRIRVFPFLREASESVAIPDS
                     GAALPGTVLVIGCSGDGTGRGDSMLVRTGDGAVVVDAVQAAGARRMAPAAMAAGRRIA
                     AGQVLCMDGVRG"
BASE COUNT          484 a          808 c          681 g          494 t
ORIGIN      
        1 ccgccagcgt tcgtcctgag ccaggatcaa actctccagt ttgatttatt gaaatggttg
       61 cgaccgaagc cccaaccgtt tctggtcatg aattgaacgg gatcattttc acgtaacctg
      121 ctattcagtt ttcaaggagc gaagccatct tcgaccgcag gcttctttgt ctcgtcgcct
      181 tgcgtccgac ttcactggtt ttcaatcagt ccgccttttc tggcggagcg ggacgggatt
      241 atgagtcagg ccctttcgac tgtcaagaag ttttttcaac tttcttttca gccaacggac
      301 cgaccatcgt cgcgccgccg ctttgaaccg attttcaaac accgcaatca gccggtttcg
      361 gcgacgcgga gcaatagaat atttccgcca ggcaaacctg tcaagaactt tctttcactc
      421 aggtcgcgtc gcctttcgac tgattgtcaa tcatcgccct gtccgagcga agcggcgcga
      481 atgataggcg gcccgtgtca tgctgtcaag cggtttttgg actatctgga aaagaattgt
      541 gcaagtgccc gttcgggaag gcagaagcat ctggtctcag tcggccacgg gcagatcgga
      601 ggtggcccat tccttgagga tcgcggcgat ttccacggcc tgctccggct tgccgatgtt
      661 gaatttcgcc tcgatgcggt acgaaccgtt gtgcttccgg tactttctta tggtgtattt
      721 cggcggccca aaggcgttct cggctctgga ccactcccgg tacttgaaga ccaggaccgg
      781 ccaggcgccc cttgcaatga tcgccttcgc ctcttccttc accacctgct gcccatcttc
      841 ctcgtactcg accgtaatgt cgtcaatctc gtcagaccac atcgcatgct ccttgcgcat
      901 cagcggccgg atccgggcgg ccgcttcctc agaacctcgg gggcgccggg cccccaatcc
      961 ttcacaatcc ccgaaagaac cgactccacc attttcaccg gccggggccg gcgcgactcc
     1021 acgaagtgct tcggcgccgc aatctggccg ggaattccac cgggcgagac gcgaagctcg
     1081 tagttgagcg aaaccggggt ctcgccaccc ccgtgggtcg atacgaactc aatccggatg
     1141 gcggcgccgg ccggagcgtt tccagacatc acaagataac ccgacagcga gttcaccctt
     1201 ccgccgaagg tctttgtcga tcccggcatc acggatgttt caaaacggca cctgtcggca
     1261 agcagcgtca acggaacccg accttcctcg tcgggctttc cctggcgtcc gcagtcatgc
     1321 gcgatgcgaa gaagggagtc ctcgccggca atgaaggaat cgaccatcgc ggacggctgc
     1381 gtgatcgcat cgtcccatcg cacccactgg ccggatgaac cggccgtgaa ccagcttcca
     1441 tccttgcggg tggccgaaaa gctctcgacc gacacgcctt ccgcggctcc cttgtgggtg
     1501 cggctccgta caaacgacat ctcgccgtcg gccagcaggt tcatcgtggc cgaatcgaca
     1561 atctccaccg tacgatcggc gcgcctgata accattgtaa gatttgattc cgcatgaaag
     1621 ccggtctgca tgaagtaccg caggctccat gccggcgtct cggccacggg cctgcccgcc
     1681 gtatcgaccg cgcctgaggc ccccttcggc cccggatcgg aacaccccgg aaccagcagc
     1741 gcaatggcaa ggacgatgac ggcaaggtca aacttcatcc acgcacccca tccatgcaca
     1801 ggacctgacc ggcggcgatc cggcgcccgg cggccatcgc ggccggcgcc atccgtcttg
     1861 ctccggccgc ctggacggca tccactacga cggctccgtc ccctgtcctg acaagcattg
     1921 aatcgccacg accggttccg tcgccggaac acccgattac aagcacggta cccgggagag
     1981 ccgccccgga atccggaatc gcgaccgatt cggatgcctc acgaaggaag ggaaataccc
     2041 tgatccttgt cccgtcgggc gtgaatgtaa acgcgccagg ccatgggtca acgcccctga
     2101 ccaggttcgc aatcgacctt gaagaggacg tccagtcgat cagaccaacg gacttgtcga
     2161 tcatcggtgc gacggtggca agcgaatgat cctgagcaat ccgcaccgcg gcaccgcagg
     2221 caagctggcc aagcgcctgc agaagcagcg gcggccccat ctgcgacagc cgttcgaaca
     2281 gggttgcggc cgtgtcggtt gcggatatcg gagtcctgcc gatcagaagg atgtcgccgg
     2341 tatcgcaacc ttcatccatc atcattattg tgacgccggt ctcggactcg ccgttgataa
     2401 ccgcccattg aatcggtgcg gcgcccctgt aggacggcaa gatgctgcca tgaacgttga
     2461 cgcagcc
//
LOCUS       DAOPPA010000477         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_46369_length_2463_cov_4.635382, whole genome shotgun sequence.
ACCESSION   DAOPPA010000477 DAOPPA010000000
VERSION     DAOPPA010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46369_length_2463_cov_4.635382"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKH54_12225"
     CDS             <1..682
                     /locus_tag="PKH54_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOD00705.1"
                     /translation="RLKGWDRVAAIDSVGASVYHATYREAWRLGLTDDLPKLFLSLVK
                     VFAYTYGFFDRLWAESPDAKVWDIKGTPQVEKRDDILRMAFKAGVAELKKKLGSNMDK
                     WCWGKLHTITFHHLFGKAAMGKFDVGPVAIPGGWDTVWAAGFGLWDDNYMFNVSEGPA
                     FRHVMDFANMKDSNMILDLGQSGWPMTPAYDNAFEHWRTGRLWKLSMDPAEFAQGAEG
                     VVELVPAR"
     gene            complement(740..2092)
                     /locus_tag="PKH54_12230"
     CDS             complement(740..2092)
                     /locus_tag="PKH54_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOD00706.1"
                     /translation="MKNEYAMRVAPALIAVLVVAGCGNPGPASGTDDGNQADIHDDAE
                     AGPEDAVRDEGHWSGYDVPGTDLGTYDAFVEEALSCNPDPIDFGSVPVGQSRTIDVVM
                     TNTQSNPISITDLYLDIDADNFEIVDLDIGDASVLDHAGELAIQVSFSPDSQGARAAN
                     LTVVGLVGGRQQMWSFQITAVGTATSGSLEFLPEVVDFGGVAIGKSKSMVMTVINNSD
                     VAVTIRSVELNQGSAGEPVRFEITRLGETLPPFEEEPVVTLDPTKFLPVEVKFTNSGP
                     DSGEYSAFLDVHVSSPNLFTYSIPVIARTAHPALCDARIVPAKLDFGTIAVGSAATGN
                     VAIVNTGTGSCLIAGGKIADCAAGPDETVTCPEVLQGSDSTVFSFDSEPGSEVIGPGA
                     RIEIGITATIPSEDNGVALSALMSFELIDVTSQTSIVLPLCADGICQPNIGATGSTSE
                     "
BASE COUNT          512 a          708 c          737 g          506 t
ORIGIN      
        1 aaggctgaag ggatgggaca gggtcgcggc catcgattcg gtcggcgcca gcgtctatca
       61 cgcgacgtac cgcgaagcct ggcggcttgg cctgacggac gacctgccga agctgttttt
      121 gagccttgta aaggtcttcg cctataccta cggattcttc gaccgcctgt gggccgaatc
      181 ccccgacgcg aaagtctggg acatcaaagg cacgccccag gtggaaaagc gtgacgacat
      241 actgcgcatg gccttcaagg ccggtgtggc cgagttgaag aagaagcttg gaagcaacat
      301 ggacaagtgg tgctggggca agctgcacac aatcaccttc caccacctgt tcggcaaggc
      361 cgcgatgggc aagttcgatg tcggtccggt cgcgataccc ggtggatggg acaccgtctg
      421 ggccgccgga ttcggcctgt gggacgacaa ctacatgttc aacgtcagtg agggcccggc
      481 cttcaggcat gtgatggatt tcgccaacat gaaggacagc aacatgatcc tggatcttgg
      541 tcagtccgga tggccgatga cgcccgccta tgacaatgcg ttcgagcact ggcgcaccgg
      601 caggctgtgg aaactgtcca tggatccggc cgaattcgcc cagggcgccg agggcgttgt
      661 tgagcttgtt cccgccagat aagcagacca ttcgttaagg ccgaccaccc gcaggaacga
      721 acaacgcatg tttcaagcct tattcgcttg ttgaaccggt agcgccgata tttggctggc
      781 atatgccatc ggcgcacaac ggcaggacaa tcgaggtctg cgacgtcacg tcgatcaatt
      841 caaacgacat aagcgccgaa agcgcgacgc cgttgtcttc ggatgggatc gtggcggtta
      901 tgccaatctc gatgcgggcg cctggaccaa tcacttcgga acccggttca ctgtcaaatg
      961 aaaaaaccgt tgaatcgctt ccctgcagga cttccggaca ggtaacggtt tcgtcgggcc
     1021 cggccgcgca gtcggcgatt tttccgccgg caatcaggca ggaaccggtg ccggtattca
     1081 cgattgcgac gttccccgtg gctgcggaac cgacagcgat ggttccaaag tcaagctttg
     1141 ccgggacaat ccgggcatcg cagagagctg gatgtgctgt tctggcaatg actggaatgc
     1201 tgtaagtgaa aaggttcggc gaactgacat gaacatccag gaacgcggaa tactcgccgg
     1261 aatctggacc gctgttggtg aacttcacct caactggaag gaactttgtg gggtcaagtg
     1321 tgacaaccgg ttcttcttcg aatggtggca gggtctcccc aagacgtgtg atttcaaatc
     1381 gaaccggttc accggcagat ccctgattga gttcgaccga acgtatagtc acagcgacgt
     1441 cactgttgtt gatgacggtc atgaccattg atttggactt cccgatcgcg accccgccga
     1501 aatcgaccac ttccggaagg aactccaggc taccggaagt cgcggttcca actgcggtga
     1561 tctggaacga ccacatctgc tgccgaccgc caaccaggcc gacgaccgtc aggtttgcgg
     1621 cccgcgcgcc ctgggaatcc ggcgaaaagc tgacctggat ggccagttcc ccggcgtgat
     1681 caagcaccga tgcgtccccg atatcgaggt caacaatctc gaagttgtcg gcatcgatat
     1741 ccagatagag gtcggttatc gaaatcgggt tggactgggt gttggtcatg acgacatcaa
     1801 tggttcgcga ctgtcccacc ggaacagagc cgaaatcgat cggatcaggg ttgcaggaaa
     1861 gcgcctcctc gacaaaggca tcgtatgttc ccaggtcggt tccgggcaca tcgtatccgc
     1921 tccagtggcc ttcatcacga actgcatctt ccggaccggc ctcggcatcg tcgtggatgt
     1981 ctgcctggtt gccgtcatcg gtgccggacg caggacccgg gttcccgcag cctgccacga
     2041 caagcaccgc gataagggcc ggagcgaccc gcatggcgta ctcattcttc atcgcacacc
     2101 ccagactacc aattgagctt gggccattgt ccaactatta acaatgcata tcaaggaagg
     2161 cggcgaaaat gcccctggcc ggggtcgaac cttgactatt ggtaacccgg gggtacaatc
     2221 cgcgcaacct cggcggggtg aatcgactct ttttgcccgg ccgtgtccca ttcaggtggg
     2281 gaagatgctt cttctggctg tttcggtgct gatcctgctt gtttcgggat tggccgcagt
     2341 cgtttccgca gttgcccgcg gcgcacgttt cgcgactgcc gccggtgtgg gcggcgctgt
     2401 gtccggttgc gtcgtcgccg catcatcggc actccacgta atcgtcaccg gtcgaacctg
     2461 gga
//
LOCUS       DAOPPA010000478         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_46558_length_2454_cov_3.709879, whole genome shotgun sequence.
ACCESSION   DAOPPA010000478 DAOPPA010000000
VERSION     DAOPPA010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46558_length_2454_cov_3.709879"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1549
                     /locus_tag="PKH54_12235"
     CDS             428..1549
                     /locus_tag="PKH54_12235"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD00707.1"
                     /translation="MVLKRRLALDSRRSYLLLGPRRVGKSTYLKTELKPDVLIDLLMS
                     DVYFDYRSRPSLLRERFSDISGTIVIDEVQRIPELVSEVHWMLENTDVRFVLSGSSAR
                     RLRREGVTNLAGRLRTQRMAPLTWQECRDHFVLEDRLQYGMLPPIVFSDDPGLDLRDY
                     CGEYLREEVQAEGLVRNLPSFAKFLEMAAFNNAELLNYSTVARDCGVSAKTVAEYYQI
                     LDDTLLGFHLEPFTRTSKRRATQTRKFYYFDCGVSNALLGRKLAPRTPEFGKVFEQFL
                     VLETIYASFYDRAVERVQFWRSSSGLEVDLLINEHTAVEFKSRRVHESDGRGIVALGE
                     EIPLKNKWIVSREELPRKLPGGIEVLPWQEYLQRLSELA"
     gene            complement(1584..>2454)
                     /locus_tag="PKH54_12240"
     CDS             complement(1584..>2454)
                     /locus_tag="PKH54_12240"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOD00708.1"
                     /translation="SLAGRAGIARLLPLSGAEIADAGLGPAEIDLWLFTGGYPALYST
                     PATRLDWLSSYVSTYLERDVRDLTAVHDLVTFQRFLRLCAARTGQLLNLTSLAADCGI
                     AQSTATAWLGILEAGYIVFRLAPHFANFGKRLIKSPKLYFHDTGLAAFLMGIQTPDQL
                     STHPARGALFETMVVSEYLRARFNRGEDSNLYFWRDSSGNEVDLMLDQAGILHPIEIK
                     SGQTVVPDMFRTLKKWQAISKSTAQPALVFGGQGRYVREGIQVTGWREMLEDHNGDKA
                     GTIPFIPSPGTGS"
BASE COUNT          494 a          665 c          768 g          527 t
ORIGIN      
        1 aagcggttca tcaggtgccg tgcgtgaaac tgtcaaaatc tgactacttc gcgcaataat
       61 agtcgccggg ttcagtgatg tcaagtcgcc gggccaggga gtgcttgcgg tggactggcg
      121 gatttcgccg gttgattttt cccgtgcggg cgaatgtcct gcaccgagcg acctgtggtg
      181 agcctgtcga accagcatag tgggtcgaag ggtcagtcat tcgccgcaac gggacgcggg
      241 gtaacacata ataccggaag tcaggaaccc gcgccgggac tggggatggc gggtgttgta
      301 tccggtttgt cgtcgggttg agacaggggg ctggttttta ctgcatccag acacgcgatg
      361 ttgacagcgg gaagcccggg ctgtattttg agaccgttac ttcggattat cctgacaaaa
      421 ggaagttatg gttctcaaaa gacgactcgc actggattcc cgacgaagct acctcctgct
      481 cggccccagg cgggtgggca agagtactta cctgaagacc gaactcaagc ctgacgtcct
      541 gatagatctc ttgatgagtg acgtctattt cgactaccga agtcggccgt cgcttttgcg
      601 ggaaaggttt tcggacattt ccggcaccat agtcatcgat gaggttcagc gcattccgga
      661 actggtttcg gaagttcact ggatgctcga gaacactgac gtccgcttcg ttctgtccgg
      721 ttccagcgcc cgcaggcttc gcagggaggg cgtcaccaat cttgccggtc gcctgcgtac
      781 acagcggatg gccccgttga cctggcagga gtgtcgtgac cactttgtgc tggaagatcg
      841 cctgcagtac ggaatgttgc cgccgattgt cttttcggac gaccctgggt tggacctgcg
      901 cgattattgc ggcgagtatc tgcgggaaga ggtccaggcg gaagggcttg tccgcaacct
      961 cccgtcgttc gcgaaatttt tggaaatggc ggccttcaac aacgccgagc tgctgaatta
     1021 ttcgacggtt gcccgggact gcggcgtcag cgccaagacg gtggccgagt actatcagat
     1081 acttgatgac acgctgctgg gatttcacct ggaaccattc accaggacaa gcaagcgccg
     1141 ggcgacccag acacgcaagt tctattactt cgactgtggc gtaagcaatg ccctgctggg
     1201 ccggaagctt gcgcccagga cgccggaatt cggcaaggtg ttcgaacagt tcctggtgct
     1261 ggaaacgatt tatgcctcgt tttatgaccg ggctgtcgag cgcgtccagt tctggcgatc
     1321 ctcgagcggc ctggaagttg atctgctgat aaacgagcac actgccgtcg aattcaagtc
     1381 gagacgggtt catgaatcgg acggccgtgg catcgtagcc ctgggcgaag aaattccact
     1441 gaagaacaag tggattgttt cgcgcgagga actgccccgc aagctgcctg gcgggatcga
     1501 agtccttcct tggcaggaat atctgcagcg actttccgaa ctcgcctgat ctccaacgga
     1561 agatttacat atttaaccgg aagttaagac cccgtgccgg gactggggat gaagggtatt
     1621 gtccccgctt tgtcgccgtt atggtcttcc agcatttcac gccagccggt gacctggatt
     1681 ccctcgcgga cataccgtcc ctgaccgccg aaaacaaggg ccggttgggc tgtggatttt
     1741 gaaatggcct gccacttctt taatgtcctg aacatgtccg gaaccacggt ctgtccggac
     1801 ttgatctcga tcgggtgaag aattccagcc tggtccagca tcaggtccac ctcgttaccg
     1861 ctgctgtcgc gccagaagta caggttgctg tcttcgccgc ggttgaatcg ggcgcgcagg
     1921 tattcggaca cgaccatcgt ttcaaacagg gccccccggg ctgggtgcgt cgaaagctgg
     1981 tcgggcgtct ggattcccat caggaaagcg gccaaaccgg tgtcgtgaaa gtacagtttc
     2041 ggggatttga tcagccgctt gccgaagttc gcgaagtgcg gcgcaagcct gaagacgata
     2101 tagccggctt ccaggatgcc gagccaggcg gtggcggtgc tttgcgcgat gccgcagtcg
     2161 gctgccagtg acgtaaggtt cagcaactgg ccggtgcggg ctgcgcacag gcgcaggaaa
     2221 cgctggaacg tgaccaggtc atgtaccgcc gtcagatccc ggacatcgcg ctcgaggtac
     2281 gtggagacgt agcttgaaag ccagtcgagc ctggttgccg gggtactgta cagcgcggga
     2341 tagccacccg tgaacagcca caggtcgatt tcggcggggc caagtccggc gtcggcaatt
     2401 tcagccccgg aaaggggcag aagcctggcg atgccggcgc gaccggcaag actc
//
LOCUS       DAOPPA010000479         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_46645_length_2451_cov_3.386060, whole genome shotgun sequence.
ACCESSION   DAOPPA010000479 DAOPPA010000000
VERSION     DAOPPA010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46645_length_2451_cov_3.386060"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2451)
                     /locus_tag="PKH54_12245"
     CDS             complement(<1..>2451)
                     /locus_tag="PKH54_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD00709.1"
                     /translation="LSAVIAGGLDIGTIIPEITELFSGITAAWNGCEVRGKNVVEAIG
                     EPEYDGFWLENNWVPDERLATAMTAWIGLGAIARAEYQDWLQHQGLLRFNDLEDRAGK
                     LLGSPAAREFIRGRFNQILVDEYQDTNQKQVDIINDIVAAAGDARVFRVGDPKQSIYR
                     FRGADVHVFNQTIADNPGDVVELTTTRRTSPALGSFFNEFFPALLDPVSLGRPDDSAR
                     IEWTSDVVCSRSSNIFPGRAVDLMLRCGAAPWAVDCDCDPVAVVGAVADADPGEAASE
                     GIALDEPAALARRIRNLLDHVKVQDNRGNSRNLTPADFAVLLRSWTHAEEFRRALEDE
                     GIPAELAGGRGLLNTPEVTDLINLIRFFANRNDPLAAAGVLRGACFGMSDLGLYVLAK
                     WPGVKRWRLDQDKWLAEPWEDTDGLNGPFKYPRNLQQVATSGCLDAEAAVGALVEAGV
                     LQETRAGDVLAQLRRDAESLCVIDADGTPVMDRGAMLLADLVDRSGYLTPSRLLEEAI
                     IGFRLEAVWMASPAGRRAVANAWKFVDLVRGVEVGGTGLQGIVAWIDSSSDPTPEGLI
                     SGVSNAVRISTIHGAKGLEWPVVAVAGLGYGFHGGSDGSWESNEVPNLDRTVAPSPVP
                     RINVSAGGFCHDADSLKKACDALLVPEDAAEEKRLLYVAMTRARDHLILSGNWAEKPD
                     VVDVDGNPSLWKCSSHKEFISSLFNLKNDKNKANWIPQAAWVKDHVNVVTVLGTGAET
                     EVATQAVGAPLPDAPVPACDLAWGTDGLDRESVNPSTARFRVADIDFSQPGDMQLLVK
                     VPDGVDDRDTGTMFHALM"
BASE COUNT          530 a          788 c          667 g          466 t
ORIGIN      
        1 tccatcagcg cgtggaacat ggtcccggtg tcgcggtcat caacgccgtc cggaaccttg
       61 accagcagtt gcatatcacc cggttgtgaa aagtcgatgt ccgcaacacg gaaacgtgcc
      121 gtgctggggt tgaccgattc ccgatccagg ccgtcggtac cccaggcaag gtcacaggcg
      181 ggtacagggg catcgggcaa tggcgcaccc acagcttgcg tagctacctc tgtttcggcc
      241 ccggttccca ggacggtgac cacattgaca tggtccttca cccaggcagc ctgcgggatc
      301 cagttggcct tgtttttgtc attttttaga ttgaacaagc ttgaaatgaa ctccttgtga
      361 ctggagcatt tccagagtga tggattgcca tcaacatcaa ccacatccgg cttctcagcc
      421 cagtttccgg acaggatcag gtgatccctg gcgcgcgtca tcgccacata cagcaggcgc
      481 ttttcttcgg ccgcatcttc cggcaccagc agggcatcac acgccttttt caaggaatcc
      541 gcatcgtggc agaaaccacc agccgaaaca ttgatccggg gaaccggaga cggcgcaacc
      601 gtccggtcaa gattggggac ctcgttcgat tcccaggaac cgtccgaacc gccatgaaac
      661 ccatacccaa gcccggccac ggcaacgacc ggccattcga gccccttcgc gccatgaatc
      721 gtcgatatcc gaacggcatt cgaaacgccc gaaatcagac cctccggggt cggatcgctg
      781 gacgaatcaa tccaggccac gataccctgc aaccccgtgc cgccaacctc gacgcctcga
      841 accaggtcaa caaacttcca ggcgttggcc acggcacgcc ggccggccgg ggacgccatc
      901 cagactgcct ccagcctgaa accgatgatg gcttcttcca gaagtcggga aggcgtcagg
      961 tacccggatc tgtcaaccag atccgccaga agcatggcgc cgcgatccat caccggcgtg
     1021 ccgtcggcat cgatgacaca cagtgattcc gcgtccctgc gcaactgggc cagtacgtcc
     1081 cccgcacgcg tttcctgcag tactcccgcc tcgacaagag ccccgacggc cgcttcggca
     1141 tcgaggcagc cggaagtggc gacctgctgc aggttgcggg gatacttgaa cgggccgttc
     1201 aaaccatccg tgtcctccca cggttccgcc agccatttgt cctgatccag gcgccagcgt
     1261 ttcacacccg gccatttggc aagcacgtac aaaccaagat cagacatccc gaagcaggcg
     1321 ccgcgaagga ctccggcagc cgccagggga tcgtttcgat tggcaaaaaa tcgaatcagg
     1381 ttaatgagat ccgtgacttc aggtgtattc agcaagccgc gcccgcctgc aagttcggca
     1441 ggtatgccct catcttcgag cgcccgcctg aattcttcgg cgtgcgtcca tgatcgcaga
     1501 agaacggcga aatcggcagg tgtcaggttc cgggaatttc cgcgattgtc ctgcaccttc
     1561 acatgatcga gaagattcct gatgcggcgt gcgagggccg ctggctcatc cagggcgatc
     1621 ccctccgacg cagcttcgcc gggatccgca tcagcgacag cgccgacaac agcaacaggg
     1681 tcacaatcac aatcaacagc ccatggcgcg gcgccgcagc gcaacatcag atcgacggcg
     1741 cgccccggaa agatgtttga actacgcgaa catacgacgt cgctggtcca ttcgattcgc
     1801 gcgctgtcat ccggtcttcc taaactgacc gggtccagca gagccgggaa aaattcgttg
     1861 aagaacgacc caagagccgg cgaggtacga cgtgtcgtcg taagttccac gacatccccg
     1921 gggttatcgg caatggtctg attgaaaacg tggacatcgg caccgcggaa acggtaaatg
     1981 gactgtttcg ggtcgccgac gcggaacaca cgcgcatcac cggccgcggc cacgatgtcg
     2041 ttgatgatgt ccacctgttt ctggttggtg tcctgatatt cgtccaccag aatctgattg
     2101 aaccgtccac gaatgaattc ccttgccgcg ggggaaccaa gcagttttcc ggctcgatcc
     2161 tccagatcat tgaaacgtag taatccctga tgctgaagcc agtcctgata ttcggcacgg
     2221 gcaatggcgc caagcccgat ccacgctgtc atggcggtgg caagccgttc atccggaacc
     2281 cagttgtttt ccagccagaa gccgtcgtat tccggttcgc caatcgcctc gacgacattc
     2341 tttccccgaa cctcgcaacc gttccaggcc gctgttatcc cgctgaacag ttcggtgatt
     2401 tccggaatga tggtgccgat gtccagacca cctgcgataa cggcagacag c
//
LOCUS       DAOPPA010000480         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_47025_length_2436_cov_2.660647, whole genome shotgun sequence.
ACCESSION   DAOPPA010000480 DAOPPA010000000
VERSION     DAOPPA010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47025_length_2436_cov_2.660647"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1476
                     /locus_tag="PKH54_12250"
     CDS             94..1476
                     /locus_tag="PKH54_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00710.1"
                     /translation="MKQLPAVLAVMCLMPIIAAVGCGDNGHRPADLPHDANVADSAGD
                     IRPDVAVDASDASGGDGVNRDNAEASDAFLDVEASTLDFGSDDFQDPGLGELADDGVD
                     PDGIADSEFDSFDSQTIDVEDVAPIQDAVTLGCEPMGPAAPVVDCAHPVEYQGGTHQT
                     CVVVPEGQCHTFWMGAVPNELYIVPMQKEVPRRMVHLTGYRMSKYLITVAEYRACVQN
                     GPCRPIPANSCLANPMAGDLGLAEPNLSDDAKLNHPVDCVSYADAETFCGWLGGELPS
                     EARFEYAAAGPMTDANQIVYFPWGSNLDSEGSELPDQVECHANIKGTTAWGDPFPQTS
                     PVGFFDGGLKTRAQGGWIGGPDTYQTCDDTGPFGTRDMTHNVLVFLKDGPTMYRDLPE
                     GQVDPEIDPVCEGVTVRATSWRYEDMVHTRVTNRHFICLVKDNMPEGVPFSRNVIRSN
                     DIGFRCAFDR"
     gene            1659..2240
                     /locus_tag="PKH54_12255"
     CDS             1659..2240
                     /locus_tag="PKH54_12255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD00711.1"
                     /translation="MAFVLPELPFDKTALQPHISAETIDFHWGKHHNAYVTNLNNLVA
                     GTAFEGKSLEEIVMASDGGVFNNAAQVWNHTFYWNCMKKDGGGKPAGKLAEAIDRDFG
                     SFDNFVKEFTAAATTQFGSGWAWLVLEDGKLKVTKTGNADLPMKHGQKAIITIDVWEH
                     AYYIDYRNARPKYIETFLQKLVNWDFAAKNFEM"
BASE COUNT          529 a          652 c          772 g          483 t
ORIGIN      
        1 gaccgctttc cctccggaac ccttgttcgc cccgtcgaat ccgtgcatta ttgaccgcaa
       61 gggtcgtgac gttcgtatta acaggaaaat ggaatgaaac agttgcccgc cgtgctggcg
      121 gtaatgtgcc tgatgccgat tatcgcggcg gtgggttgcg gcgataatgg acatcgtccc
      181 gctgatttgc cgcacgacgc gaatgtcgcc gattcagcgg gcgacatccg gccggacgtt
      241 gccgtcgacg catccgacgc ttccggcggg gacggcgtga accgggataa tgcagaggcg
      301 tccgatgcgt ttctggatgt ggaagcctca accctggact tcgggagtga tgactttcaa
      361 gatcccggcc tgggcgaact ggccgacgat ggcgtggatc cggacggcat tgcggattcg
      421 gaattcgatt catttgattc ccagacaatc gatgtcgagg atgtcgcccc gattcaggat
      481 gccgtgacac ttgggtgcga gcccatgggt ccggccgcac ccgtcgtcga ttgcgcgcat
      541 cccgtcgagt atcagggcgg gacccaccag acttgcgtcg tcgtccccga aggccagtgt
      601 cacaccttct ggatgggtgc cgttccgaat gaactttaca tcgtaccgat gcagaaggag
      661 gttcccaggc gcatggttca cctgaccggg tacaggatgt cgaagtacct gattaccgtt
      721 gccgagtaca gggcctgcgt ccagaacggc ccgtgccggc cgatacccgc gaattcctgc
      781 ctggcgaatc cgatggccgg tgaccttggc ctggccgaac cgaacctttc agacgacgcg
      841 aagctgaacc atccggtcga ttgcgtgtct tacgcggatg cggaaacctt ctgcggctgg
      901 ctcggcggcg aactgccatc agaggccagg ttcgagtacg ctgcagcggg accgatgaca
      961 gacgcgaatc aaatcgttta tttcccgtgg ggaagcaatc tggattcaga gggaagcgag
     1021 cttcccgatc aagtcgagtg tcatgcaaat ataaagggta cgaccgcctg gggcgatccg
     1081 ttcccgcaga cgtcgcctgt cggattcttt gacggcgggt tgaagacgcg ggcccagggc
     1141 ggctggatcg gcggccccga cacgtaccag acctgcgacg acaccgggcc gttcgggacg
     1201 cgcgacatga ctcacaacgt gctcgtattc ctcaaggacg gtccgacgat gtacagggat
     1261 ttgccggagg gccaggtgga tcccgagatc gatccggtat gcgagggcgt gaccgtgcgc
     1321 gcgacttcat ggcggtacga ggacatggtg cacaccaggg tgaccaaccg gcatttcatc
     1381 tgccttgtta aggacaacat gccggaggga gtgccatttt caaggaatgt gatacgttcg
     1441 aacgatattg gattccggtg tgcctttgat cggtgatcct cttctcgaca acgcgggagg
     1501 ttttgatgaa catcgcaagt ccacggggtc tgaaggtttt cgggttcagc attgcggcgg
     1561 ccgccgggtt cgctgctatg cttgtgtttg ccgccggaac tgccgaagca aggcagacgg
     1621 ctgcaaagaa ggacattccc aagcaagagg tgaagaaaat ggctttcgta cttcctgaac
     1681 tgccctttga caagactgcg ctgcagccgc acataagcgc ggaaacgatc gatttccact
     1741 ggggaaagca tcacaacgcg tatgtcacga acctgaacaa tcttgtcgcg gggacggcct
     1801 ttgaaggcaa gtcgctggaa gaaatcgtta tggcttcgga tggcggagtg ttcaataacg
     1861 ccgcccaggt ctggaaccac accttctact ggaattgcat gaagaaggac ggtggcggca
     1921 agccggccgg caagcttgcc gaggcgattg accgcgattt cggcagcttc gacaatttcg
     1981 tgaaggagtt caccgcggcg gcgacgaccc agttcggaag cggatgggcc tggctggtgc
     2041 tggaagatgg aaagctgaag gtaacgaaga ccggaaacgc ggatctgccg atgaagcacg
     2101 gacagaaggc gataatcacc atcgacgttt gggagcatgc ctactacatc gattaccgta
     2161 acgcccgtcc gaagtacatc gagacgttcc tgcagaagct ggttaactgg gacttcgcgg
     2221 cgaagaattt tgaaatgtag cggcggaaat ttcgcctggg gcatcgtgca gaatcgcagc
     2281 gggcggatgt aatccgcccg tagaagtgcg ggaaattcgc ccgtacaagg gcgggaaatt
     2341 cgcccgcttt aatggagatc aaccaacatg aaacgtaagg caagcgcggt atggaacggt
     2401 ggactgaagg acggaacggg cactttcagc gtcggc
//
LOCUS       DAOPPA010000481         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_47617_length_2415_cov_3.875000, whole genome shotgun sequence.
ACCESSION   DAOPPA010000481 DAOPPA010000000
VERSION     DAOPPA010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47617_length_2415_cov_3.875000"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKH54_12260"
     CDS             complement(<1..391)
                     /locus_tag="PKH54_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00712.1"
                     /translation="MNSTRRLFAILLIHVITAVAALPALAHQPCPPADGEIVGVLKAP
                     DGSFDVHVSARGGVLKAVILHDKQFHQTAREPFKGTVQPPSQKIAEGPLDLVSTWDNA
                     FLPLTIAFEDPSPLSMGRLVKVVTPPVA"
     gene            complement(388..621)
                     /gene="yidD"
                     /locus_tag="PKH54_12265"
     CDS             complement(388..621)
                     /gene="yidD"
                     /locus_tag="PKH54_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOD00713.1"
                     /translation="MPVILLTGLIQAYRLLISPLLGPACRFSPTCSEYMIQALKIHGF
                     FRGLWLGIRRIGRCHPFNPGGFDPVPERKTVTK"
     gene            complement(581..1039)
                     /gene="rnpA"
                     /locus_tag="PKH54_12270"
     CDS             complement(581..1039)
                     /gene="rnpA"
                     /locus_tag="PKH54_12270"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD00714.1"
                     /translation="MDRGFPRSNRLLRPSDFERVMKGGFRAWTWNLIVFVKENDLECS
                     RLGLATGRKVGNAVARNRWRRLIREVFRNELKDSLAGFDMVVAVKAVPGFERNRGEAA
                     ERRPDGPSAKREIPAIADLEGELADALRRIERKLAGKVAGNPSDRPDPGV"
     gene            complement(1048..1197)
                     /gene="rpmH"
                     /locus_tag="PKH54_12275"
     CDS             complement(1048..1197)
                     /gene="rpmH"
                     /locus_tag="PKH54_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD00715.1"
                     /translation="MKRTYQPSRIKRLRTHGFRARMATAVGRKVLSRRRAKGRAVLVP
                     TIPGK"
     gene            complement(1382..>2415)
                     /locus_tag="PKH54_12280"
     CDS             complement(1382..>2415)
                     /locus_tag="PKH54_12280"
                     /inference="COORDINATES: protein motif:HMM:NF020742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOD00716.1"
                     /translation="GHTAHESAEEAEQETLRMLECYRSFAEDFLAVPVMTGVKTDSEK
                     FAGALRTYCIEGMMQDGKALQMGTSHNLGQNFARAFDIKFEGRDQVVQNAWTTSWGVS
                     TRLIGALIMAHSDDEGLVLPPKVAPVLAVVIPIGKSDEEKKKVVEYFNPVLRRLAGDA
                     EVDAASARLGNDQLSAVFYNNLTGQQIVVDMRDQMRPGEKHYYWEQRGVPLRIEIGPR
                     DVESGTAVIKSRLGDKDVINAADLDRNSFDSRLAAIQDALFRRAVEFRNANVVDVASY
                     DELKAFFAGSRGFVRCRFNPDRAVEARIKEETKATVRCIPFDQPGGTGPDIISGKPAD
                     RVVVFGIGY"
BASE COUNT          505 a          764 c          664 g          482 t
ORIGIN      
        1 cggcaaccgg cggcgtgacc accttgacaa gacgccccat ggaaagcggc gacggatcct
       61 cgaaggcaat cgtcagggga agaaaggcat tgtcccacgt cgaaacaaga tccagtggcc
      121 cctcggcaat cttctgcgaa ggcggctgaa cggttccctt gaaaggttcc cttgcggtct
      181 ggtgaaactg cttgtcgtgg agaatgacgg ccttcaggac accgcccctg gctgaaacgt
      241 ggacatcgaa actgccgtcc ggggccttca ggacaccgac aatctcgccg tcagccggcg
      301 gacatggctg gtgcgcaagt gccggaagcg ccgcgaccgc ggtgatgaca tgaatcagga
      361 ggatcgcaaa caggcgtctg gtcgaattca ctttgtcacc gttttccttt ccggaacagg
      421 atcaaaaccg cccggattaa agggatggca tctgccgatc ctgcggatcc cgagccacag
      481 cccgcgaaaa aatccgtgga tcttcagtgc ctgaatcatg tactcggaac aggtcgggct
      541 gaagcggcag gcggggccca gcaacggaga aatcagcaat ctatacgcct ggatcaggcc
      601 tgtcagaagg attaccggca accttgccgg ccagctttct ttcaatccgg cggagggcat
      661 cggccagctc cccttcgagg tctgcaatgg cggggatctc ccgcttcgcg gatgggccgt
      721 ccggcctgcg ttccgcggct tcgcctcggt tgcgttcaaa cccgggaacc gccttcaccg
      781 cgacaaccat gtcgaagccg gccagactat ccttcagttc gttcctgaaa acttccctga
      841 tcagtcttct ccagcggttg cgggcaacgg cgttcccaac ctttctgcca gtcgccagcc
      901 cgagccggga acattccagg tcgttttcct tgacgaaaac aatcaggttc caggtccaag
      961 ccctgaaacc gcccttcatc acccgctcaa agtcggatgg acgaagaagc ctgttcgacc
     1021 tgggaaatcc ccgatccacg gcttgcgcta cttgcccgga atggtcggaa cgagaacggc
     1081 gcggcccttt gcacgacgcc tcgacagcac cttgcggcca acggccgtgg ccattctggc
     1141 ccggaatccg tgggtcctga gtcgcttgat acggcttggc tgataagttc ttttcatgac
     1201 tgaattccct taaataatca acaggttcaa ccaacttcag aaggctttcg aacctgctgg
     1261 aaagagtctc tgccgcccaa cctgcaggcg cacagcgaag tattttatgg atatcctggc
     1321 ttgtgtcaat ggaagacctg tcatgagtca ccgtccgaaa acaacaggct tccgaaatcg
     1381 atcaatagcc aatgccgaaa acgactaccc tgtcggccgg ctttccggaa attatgtccg
     1441 gccccgtgcc gccgggctgg tcaaacggta tgcaccggac cgtcgccttt gtctcttcct
     1501 tgatcctggc ctcgacggcg cgatccggat tgaagcgaca acggacaaag ccgcggcttc
     1561 cagcaaagaa ggccttcaac tcgtcatacg aagccacatc aaccacgttt gcgttgcgga
     1621 attctacggc acgccggaac aacgcgtcct ggatggccgc cagccgcgaa tcgaacgaat
     1681 tcctgtcaag gtcggccgca ttgatgacgt ccttgtcgcc cagcctggac ttgatgaccg
     1741 cggttccgga ctcgacgtct ctcggaccga tttcaatccg cagcgggacg ccgcgctgtt
     1801 cccagtaata atgcttctcg ccgggacgca tctgatcgcg catgtcgacc acgatctgct
     1861 ggccggtcag gttgttgtag aagaccgccg agagctggtc atttccaagc ctggccgacg
     1921 cggcatcgac ctcggcatca cccgccagac gccggagcac cggattgaag tactcgacaa
     1981 ccttcttttt ctcttcgtcg gacttgccaa tcggaatcac gaccgccagc acgggggcca
     2041 ccttcggtgg caggacaaga ccctcgtcgt cgctgtgggc catgatcagc gcgccgatca
     2101 aacgggtcga tacaccccac gaagtcgtcc atgcattctg aaccacctgg tcgcgcccct
     2161 cgaacttgat atcgaacgcc ctggcaaaat tctggcccag gttatgggat gtgcccatct
     2221 gaagggcctt cccgtcctgc atcatgcctt cgatgcaata cgtccgcagc gcgccggcaa
     2281 acttctcgga gtccgtcttg acgccggtca tgaccggaac cgccaggaag tcctcggcga
     2341 aggaacggta gcattcgagc atcctcaagg tttcctgctc cgcttcctcg gcgctctcat
     2401 gggcggtgtg accct
//
LOCUS       DAOPPA010000482         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_47667_length_2413_cov_5.074640, whole genome shotgun sequence.
ACCESSION   DAOPPA010000482 DAOPPA010000000
VERSION     DAOPPA010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47667_length_2413_cov_5.074640"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKH54_12285"
     CDS             complement(<1..249)
                     /locus_tag="PKH54_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00717.1"
                     /translation="MRWLPVLMVTILLVGCGGTGMNPMSCAGKILKKGQVQEQGQALQ
                     DGEDIAPVTVADPIGEAEIRAFIDRWLATQNDGRFQDYQ"
     gene            410..482
                     /locus_tag="PKH54_12290"
     tRNA            410..482
                     /locus_tag="PKH54_12290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Thr,seq:cgt)
     gene            662..1717
                     /locus_tag="PKH54_12295"
     CDS             662..1717
                     /locus_tag="PKH54_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00718.1"
                     /translation="MQFSGNIKLLDDVLRIVQARLPLGWHLQQIARDGTSGSDIELIY
                     PGQAGVSFKAEIRAALDPRGVIQLKAGMASETKGAAGSRPVLVISRYLAPATRQKLVE
                     AGFNFADLTGNVRIVLASPAIFIELSGADRSPFREDRKSRTLRGPKVGRIVRALVDFA
                     ETPGVRELAVRAGVDAGYLSRVLTLLEREALIDRGHRGCVVRLDWPGLLRRWASDAPL
                     ESRGEVMMFIDPRGLDSVVTKLATSGTRYAVTGSLAAGRVSQVAPARLAMIYVDDIDA
                     ASGFLGLRPAEAGANVVLVRPVDDVVYERSFVADGVRYAAVSQVAADLLTSPGRGPAE
                     AEELLNWMEANQKVWRG"
     gene            1710..1970
                     /locus_tag="PKH54_12300"
     CDS             1710..1970
                     /locus_tag="PKH54_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00719.1"
                     /translation="MDNLYILARRVLLDALGALGSHRDTVILVGAQAVYLRTGEADLA
                     VAPYTTDGDLVLDPSVLAEAPPVEAALRTAGFLPRSGESVGA"
     gene            2008..>2413
                     /locus_tag="PKH54_12305"
     CDS             2008..>2413
                     /locus_tag="PKH54_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00720.1"
                     /translation="MLVDADFMTLNALEPNDHRTFDIRVAGPAALLVAKMYKLNDRKG
                     SDRLSDKDALDVLRLLRGTSTEDMCRRMKNMQKDDRSVAPAAGAIKFMQDLFGRENAP
                     GIQMAVRSAGMLANPEEIAVSCLLLATDLLNEL"
BASE COUNT          454 a          688 c          735 g          536 t
ORIGIN      
        1 ctggtagtcc tgaaacctgc catcattctg ggtcgccagc cagcgatcga tgaatgcccg
       61 gatctcggcc tcgccgatcg ggtccgccac cgtcaccgga gcgatgtctt ccccatcctg
      121 cagcgcctgg ccctgttcct ggacctggcc cttcttcaga atctttccgg cgcacgacat
      181 ggggttcatc ccagtgccgc cgcatccgac cagcagaatc gtgaccatca acacgggaag
      241 ccaacgcatg ggcaaaacct ttttcagata gggtggttat atccttgcca aaccgggcgc
      301 gtcaaggccc gccgtggtcg ccgttcggcg tcccgatttg acccgttgtc cccttttgtt
      361 cttgacaaat ccagtcgttt caggaaaatc ccctctgcct tttggcggcg ccaacgtagc
      421 tcagtcggca gagcacttga ttcgtaatca gggggtccgg ggttcgattc cccgcgttgg
      481 ctctaataat tcaagtagtt acgtccttta attcccccga caaatgcctt tgtccatggg
      541 catcgcggtc aaatcccgca agttaatccg aggttgtgtc taccccggcc aagtctcctg
      601 accgccagga ccttgcaatt gtttgttgtc taattataag atgttgttcg aggtgggcaa
      661 catgcaattt tcaggcaaca tcaagcttct ggacgacgtg ctccggattg ttcaggccag
      721 acttccattg gggtggcatt tacagcaaat agcgcgtgat ggcacgtccg gttcagacat
      781 tgaactgatc tatcccgggc aggctggcgt gagcttcaag gctgaaatcc gcgccgccct
      841 ggatccccgt ggggtgattc agcttaaggc agggatggcg tccgaaacaa aaggcgccgc
      901 cggctccagg ccggttctgg tcatatccag ataccttgcc ccggccacaa gacagaaact
      961 tgtcgaagcg ggattcaact tcgcggacct gacggggaat gtccgcatcg tgctcgccag
     1021 tccagcgata tttattgaat tgtccggcgc cgaccgaagc cccttccgtg aagaccgtaa
     1081 gtcacgcacg ctgcgtggtc cgaaggtcgg caggatcgtc cgggctcttg tagatttcgc
     1141 cgaaacgccc ggggttcggg aactggcggt cagggccggg gtcgacgccg gttatctgag
     1201 ccgggtcctg actttattgg aacgggaagc cctgattgat cgaggacaca ggggatgcgt
     1261 cgtcaggctg gactggccag gccttctgag gcgctgggca agtgacgcgc cgctcgaatc
     1321 aaggggcgaa gtaatgatgt tcatcgaccc ccgtggattg gattccgtgg tcacgaagct
     1381 tgcaacgtcc ggcacacgat acgctgtcac cggttcgctt gcggccggca gggtttcgca
     1441 ggttgccccg gccaggcttg cgatgattta tgtggatgac atcgatgccg cttccggatt
     1501 tcttggtctg cggcctgcgg aagccggggc caacgttgtt cttgtgcggc cggtcgacga
     1561 cgttgtttac gaacgttctt ttgttgcgga tggcgtcagg tatgccgctg tttcacaggt
     1621 cgccgccgac ctgctcacca gcccgggccg gggccctgcc gaggctgaag aacttctgaa
     1681 ctggatggaa gcaaaccaga aggtctggcg tggataatct gtacatcctg gcccggcgtg
     1741 tgttgctgga tgccctgggg gcgctgggtt cccacaggga tacggtgatc ctggtcggtg
     1801 ctcaggccgt ctatctgagg accggcgagg ctgaccttgc cgtggcaccg tacacgacgg
     1861 acggcgacct ggttctggac ccttctgttc tggccgaagc gccaccagtt gaagccgctt
     1921 tgaggactgc cggtttcctg ccaaggtcag gcgagtccgt cggggcatga cccgaagctt
     1981 gcccgcatcg tcgagggtct tgagggggtg ctggtcgatg ccgacttcat gacgttgaac
     2041 gctctggagc ccaacgacca tcggacattc gatatcaggg ttgcgggtcc ggccgcgctg
     2101 cttgttgcca agatgtacaa gctgaatgat cgaaaaggaa gcgacaggtt gagcgacaag
     2161 gatgcactcg acgtcctgcg gctgcttcgc ggcacatcga cggaagatat gtgccgtcgc
     2221 atgaagaata tgcagaagga tgaccggtcg gtcgcccctg ccgccggtgc aatcaagttc
     2281 atgcaggatt tgtttggacg tgaaaatgca cccgggatcc agatggcggt cagatcggct
     2341 ggcatgcttg ccaatcccga agaaattgca gtgtcctgcc tgctgctggc gacggacctt
     2401 ctgaacgaac tgc
//
LOCUS       DAOPPA010000483         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_47726_length_2411_cov_3.976231, whole genome shotgun sequence.
ACCESSION   DAOPPA010000483 DAOPPA010000000
VERSION     DAOPPA010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47726_length_2411_cov_3.976231"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PKH54_12310"
     CDS             <1..1843
                     /locus_tag="PKH54_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOD00721.1"
                     /translation="DPQAAVEKWMSLGVLDAAEAADALEKIIDGRQGARRFHSIVVKR
                     NLVGTRCPNDLEPLRFDAMTGQMVCSRCGGSFVDQKAVVQAHLHEMKGFSLHSRMVRH
                     YPAGAATIHAVGYVNEVTAAEVDDSNGVLKPGDVIGRSGLEKALDEELRGKVGEDVYV
                     RSAAGSRIRPADLPEPFKDLKSFPPVPGRSVKTTLELGLQKAAVAALKPHRSGAIVVM
                     DVDTGEILAMASQPTFELGPRLTVADREPPPLQVDYAPMMNKAVSSFPPGSTFKVVTA
                     LAGLMEGVIDSGTRTDCPGYYEYRNRKFRCFVRSGHGEVDLVDSLAQSCDTYYYRLGD
                     LLGIDTIARYATEMFGLGQKTGIEISENPGVIPTEAWYLNSSKGYQPGFAINASVGQG
                     DIRTSPLSMARAYAVLANGGRIVQPRLTAAWIDPDTGLEEPVATVVGATVQLHDRHRD
                     LIERGLYGAVNTEAGTAYASRIRELPYSGKTGTAEAREYRKDAGPELQQWLKGDHAWF
                     VALAPARRPRIAVAVFLEHAGFGGAVAAPAARKVIEAYYREHADEFIDLWEGFDSRPE
                     LEIVHEGPAPDQESGTDSSVDSGESVTASDQDGSQQPEKAGADDSQE"
     gene            1827..>2411
                     /locus_tag="PKH54_12315"
     CDS             1827..>2411
                     /locus_tag="PKH54_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOD00722.1"
                     /translation="MIRKSDRRPFSRAVWVAAAALFTILVVGLVNLKNAASYTGEDFH
                     QTQILWVVAGCVIAFAVAVIDLRFFQQLAPLFFWGAVVLLVATLIFGREINHSRRWIL
                     GIQPSEFVKVAMVMMLARFFHGERNPERYTLRTLLRPLGLILVPMILILMQPDLGTAL
                     CVMAIGFSVMFFEGIRLRSFLGLLAIVVLVVPLAW"
BASE COUNT          449 a          675 c          793 g          494 t
ORIGIN      
        1 cgacccacag gccgcggttg aaaagtggat gagccttggc gtcctcgatg cggcagaggc
       61 ggccgacgcg cttgagaaga ttatcgacgg aaggcagggg gccaggcgtt ttcattcgat
      121 tgtcgtgaag cgcaacctcg tcggcacgcg ctgcccgaac gacctggagc cactgcggtt
      181 cgacgccatg accggccaga tggtctgttc aaggtgcggc ggttcgtttg tcgatcagaa
      241 ggcggtcgtt caggcgcatc ttcatgaaat gaagggtttt tcgcttcatt cgaggatggt
      301 tcgccattat ccggcgggcg cggccaccat acacgcggtc ggatatgtca atgaagtcac
      361 cgcggccgaa gtcgatgatt cgaacggagt cctgaaaccc ggtgacgtga tcggccgttc
      421 gggactggaa aaggccctgg acgaagaatt gcgcggaaag gtcggcgagg acgtctatgt
      481 ccgcagcgcg gcgggaagca ggatcaggcc ggccgacctt ccggaacctt ttaaggatct
      541 taagtcgttt ccgccggtac cgggccgcag cgttaagacg acgctggaac ttggcttgca
      601 gaaggccgcg gttgccgcct tgaagccgca cagatccgga gccatagtcg tcatggacgt
      661 ggataccggt gagatccttg ccatggccag ccagccgacg ttcgagcttg gccccaggct
      721 gacggtagcc gacagggagc cgccgccgct ccaggttgat tatgccccga tgatgaacaa
      781 ggccgtttcc tcgtttccgc ccggttcgac tttcaaggtc gtcacggccc tggccggatt
      841 gatggagggc gtgatcgatt ccggcacgcg gaccgattgc cccggttatt acgaataccg
      901 taaccggaag ttcaggtgct tcgtgcgcag cggtcatggc gaggttgacc tggtcgattc
      961 gctggcgcag tcatgcgaca cttattacta ccgcctgggc gacctgcttg gaatcgacac
     1021 gatcgcaaga tacgcgaccg agatgttcgg ccttgggcag aagaccggga tcgagatttc
     1081 cgagaatccc ggagtcattc cgaccgaggc atggtacctg aacagttcca agggttacca
     1141 gcctggcttt gccatcaacg cgtctgtcgg gcagggtgac ataagaacca gtccactttc
     1201 catggcgcgg gcgtacgcgg tgctggcaaa cggtggcagg attgtgcagc caaggctgac
     1261 ggcggcctgg atcgacccgg ataccgggct cgaggaaccg gtggctacgg tagtcggcgc
     1321 gacggttcaa ctccatgatc ggcaccgcga tctgatcgaa cgcggacttt atggggccgt
     1381 caacaccgag gctggaacgg catatgcatc caggattcgc gaattgccgt attccggcaa
     1441 gaccggtacg gccgaggctc gcgaatatcg taaggacgcg ggcccggaac tgcagcaatg
     1501 gctgaagggc gatcacgcgt ggttcgtggc cctggcgccc gcccgccgtc caaggattgc
     1561 ggtcgcggta ttcctggaac atgccggatt cggcggcgcg gtcgctgcgc cggcggccag
     1621 gaaggtgatc gaggcgtact acagggaaca cgccgacgaa ttcatcgatt tgtgggaagg
     1681 gttcgacagt cgtccggaac tggagattgt tcacgagggc ccggccccgg atcaggagtc
     1741 cggaaccgat tcctcggttg actccgggga gtcggtgacg gcatcggatc aggacggctc
     1801 gcagcagccg gaaaaggcgg gggcagatga ttcgcaagag tgacagaagg ccgttttcaa
     1861 gggccgtctg ggtcgccgcc gcagccttgt tcaccatact ggtggtcggt ctggtcaacc
     1921 tcaagaatgc cgcttcatat actggcgagg atttccacca gacccagatt ctgtgggttg
     1981 tcgccggctg cgtgattgcc ttcgcggttg ccgtcatcga tctgcggttc ttccagcaac
     2041 tggcgccgct gtttttctgg ggagcagtcg tgcttctggt cgcgaccctg atattcggac
     2101 gcgaaatcaa ccattccagg cggtggattc tgggaattca gccatccgag ttcgtcaagg
     2161 tggcgatggt gatgatgctt gccaggttct tccacgggga acggaatccc gagagataca
     2221 cgctccggac gctcctgcgc ccgcttggac tgatccttgt tccgatgatc cttatcctga
     2281 tgcagcccga ccttggaacg gccctctgcg tgatggcgat cggtttctcg gtgatgtttt
     2341 ttgagggcat caggctgcgt tccttcctgg ggctgctggc catcgtggtc ctggtggtcc
     2401 cgctcgcctg g
//
LOCUS       DAOPPA010000484         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_47962_length_2401_cov_3.270673, whole genome shotgun sequence.
ACCESSION   DAOPPA010000484 DAOPPA010000000
VERSION     DAOPPA010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47962_length_2401_cov_3.270673"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKH54_12320"
     CDS             <1..941
                     /locus_tag="PKH54_12320"
                     /inference="COORDINATES: protein motif:HMM:NF009239.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HOD00723.1"
                     /translation="RNDISMFCPGRENEMLDSIVAGNKGPFSDAVIRQIFKEIFRASL
                     ESMQAAGIEHLKVSRAQGEQDVVIDVRGHLIGRDPVVIAGPCAIENAAQASEVARHLV
                     SRKIHFMRGGAFKPRTSPYSFQGLGVEALGILDGIRTDFDMVVVTEVTDTRSVDAVAA
                     AVDILQIGSRNMANYELLRAAGATGKPILLKRGFAATLDEFMLAAEYVSREGNENIIL
                     CERGIRTFARETRYTLDISAVPLLRQMSRLPVIVDVSHAAGRRDLVIPLAKAAMAAGA
                     SGVMVEVHPSPAHALSDSAQQIDLGEFDELIAALKR"
     gene            complement(1148..>2401)
                     /locus_tag="PKH54_12325"
     CDS             complement(1148..>2401)
                     /locus_tag="PKH54_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD00724.1"
                     /translation="RRFLDGFAKRIVELSRRYPEGSFMSIGNYQAFLDAQADWFESEA
                     KSRLSLETRVKRETEWLMRGPKARATKARYRVDEAHRLIDELNTARERGRVAETDFEF
                     TGTGSKTKRLMVVRNLAKSLGGKPLFSGLDLIVSPGMRIGLVGPNGSGKTTFLRLLAG
                     ELEPDDGSVRTAPSLNVVYFDQKREKLDPAQTLRRTLAPDGDSVFYRDEPIHVQSWAS
                     RFRFRRDQLDSPIGSLSGGEQARALIACLILRKCDLLLLDEPTNDLDIPTLEVLEESI
                     VTFPGAVMLVTHDRYMLDRVTTVTLGLDGLGGVSAYADFEQWYAVMQEAAKPARDKAP
                     ARERPKQKKKLTYLEDKELKGMEDAISAAEAALEKARARLGDASIATNPGELQKACQA
                     EGLAAAEVERLYSRWSELEEKQNSD"
BASE COUNT          435 a          770 c          717 g          479 t
ORIGIN      
        1 aacgcaacga tatcagcatg ttctgccccg gacgtgaaaa cgagatgctt gactcaatcg
       61 ttgccggcaa caagggcccg ttttcggacg cggtcatccg ccagatattc aaggagatat
      121 tcagggccag cctggaaagc atgcaggccg cggggatcga gcatctgaag gtgtcgcgcg
      181 cccaaggcga gcaggatgtc gtcatcgacg tgcgcggcca cctgattggc cgtgatccgg
      241 tcgtgatagc cggtccctgc gcgattgaga acgcggccca ggccagcgaa gtcgcccgtc
      301 acctggtctc gcgcaagatc catttcatgc gtggcggggc tttcaagccg cgcacgtctc
      361 cgtattcgtt ccaggggttg ggcgtagaag cccttggcat tctggatggc atcagaaccg
      421 acttcgacat ggtcgtggtc accgaggtca ccgacacaag gtctgtcgat gcggtcgccg
      481 ccgcggtcga tatcctgcag atcggttcgc gcaacatggc caattacgag cttctgcgcg
      541 cggccggtgc gaccgggaag ccgattctgc tgaagcgcgg gttcgcggcg accctggacg
      601 agttcatgct ggcggcggag tacgtatccc gcgaaggaaa cgaaaacatc atcctgtgcg
      661 agcgcggtat caggacgttc gcaagggaaa ccagatatac cctggacatc agcgcggtgc
      721 ccctgcttcg ccagatgagc cgactgcccg tcatagtcga tgtcagtcac gcggccggtc
      781 gccgcgatct ggtgattccg ctggccaaag ccgcgatggc ggccggggct tcgggtgtga
      841 tggtggaagt ccatccgtcc ccggcgcacg cactgtcgga cagcgcccag cagattgacc
      901 ttggcgagtt tgacgaactc attgctgcat tgaaacggta gggcgacgga cccgccttcg
      961 ccggggcgaa tgataattcg cccgtcagat tgaaggcatc gtgccggacc gcagcgggcg
     1021 gatgtaatcc gcccgtacaa ggggcgaatg aaaattcgcc ccccacgtga aaattcgccc
     1081 gaaacgtgga aattcgcccg catgcgtgaa aattcgcccg tacccgccgc gagtcgcgca
     1141 catctttcta gtcggagttc tgcttttctt ccagctcgga ccagcgggag taaagccgtt
     1201 cgacctcggc cgcggcaagg ccttccgcct ggcacgcctt ctgcagttcc ccgggattgg
     1261 tcgcgatcga cgcgtcgcca agcctggcgc gggccttttc aagcgccgcc tcggccgccg
     1321 aaatcgcgtc ctccattccc ttcagttcct tgtcttcaag gtaggtcaac ttcttcttct
     1381 gtttcgggcg ttcgcgggcc ggagccttgt cccgtgcagg cttcgcggcc tcctgcatca
     1441 cggcgtacca ctgttcgaaa tcggcgtacg ccgaaactcc gcccagtccg tccaggccca
     1501 gcgtcaccgt ggtcacgcgg tcgagcatgt acctgtcgtg cgtgaccagc atcacagcac
     1561 ccgggaaggt cacgatgctt tcctcaagca cttccagcgt cgggatgtcc agatcgttcg
     1621 tcggttcgtc aagcagcagc aggtcgcact tgcgaagtat caggcacgcg atcagtgccc
     1681 tggcctgctc gccgccggac aggctgccga tcggcgaatc aagctgatcc cgcctgaacc
     1741 tgaagcgcga agcccacgac tgaacgtgga tcggctcgtc gcggtaaaag acggagtcgc
     1801 catcgggcgc cagcgtccgt cgaagtgtct gcgccggatc gagtttttcc cttttctggt
     1861 cgaaatagac cacgttcagg ctaggcgcgg ttcgcaccga cccgtcgtcc ggttccagct
     1921 cacccgccag cagccgcagg aaggtcgtct tgccggatcc gttcgggccg accagcccga
     1981 ttcgcattcc aggtgacact ataagatcca gtcccgaaaa caggggcttg ccgcccagcg
     2041 acttcgccag gttgcgaacg accatcagcc gcttcgtctt gcttccggtg cccgtgaact
     2101 cgaaatcggt ctccgcgacc cggccgcgct cgcgcgcagt gttcagttcg tcaatcaggc
     2161 gatgcgcttc atccacgcgg tacctggcct tggtcgcgcg cgccttcggt ccgcgcatca
     2221 gccattcggt ctcgcgcttg acccgcgttt ccagtgacag cctgcttttt gcctcgcttt
     2281 caaaccagtc cgcctgggcg tcaaggaagg cctggtagtt gccaatcgac atgaaactgc
     2341 cttccgggta acggcggctg agttccacga tcctttttgc gaacccgtcc aaaaatcttc
     2401 t
//
LOCUS       DAOPPA010000485         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_48158_length_2393_cov_2.997006, whole genome shotgun sequence.
ACCESSION   DAOPPA010000485 DAOPPA010000000
VERSION     DAOPPA010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48158_length_2393_cov_2.997006"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PKH54_12330"
     CDS             complement(<1..1459)
                     /locus_tag="PKH54_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00725.1"
                     /translation="MVSSISRYLVAVGIVFFFGGLASGCGGDGEIFDAIDDVATDSVS
                     HPEVDSGEDAGQDTRPDVETPDDVADTVDGNAPDVTGDADVGIPDAFDHGVDPDAATD
                     AEADADPDADDVEIDTGNPCIPDCSATVCGLDPVCGTLNCGDCIGAQCVLGQCVNNGC
                     YRADLGRCVDSYSSYEDPPEEFLSSHEEFCVEDGGVWGLCPAQGRLLSAKFTYSGGGG
                     YTLHYYHGLYCDSGEAVVVAEAKAQRERDLLQAELVVSYPFEDPSAHCSAVGADRIAC
                     QLPGISAPCLELGGGIAPERGAAFSDVCTGFFGGTVDSACPFTEVGVCERGIDAGVPD
                     YRLKYVYDPLDKQSEATVCNREGGWWLAVECTDQDTPHGTCQLNVHSCGHQAGEYEPA
                     SFAATVVEKVAGTTGYSCCHEDPQTAYDQALADLLTRDDLERCEPCVPDCTGIVCGVD
                     PVCGQVCGGCTEPGQACVQNACVVVGCNVPAQGICY"
     gene            complement(1778..>2393)
                     /locus_tag="PKH54_12335"
     CDS             complement(1778..>2393)
                     /locus_tag="PKH54_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00726.1"
                     /translation="AITFQNNAVCWGDNQSGQLGVGNTDNLLKPTWLVSETGMTEWIS
                     AGGQHTCSLGRVGIGYGQYLYQMECWGSDSTGQIAGEGNSACATGSCRTSPATAAFWQ
                     ALMSLPDPPVPAAGAFHTCYVDSFNQLFCVGHNDFGQIGNDTIGGIQVFYKVFPSGAS
                     AVTAGGRHTCAIVDSRLYCWGRNSDGQIGDTTQETRLVPTPVSW"
BASE COUNT          524 a          769 c          666 g          434 t
ORIGIN      
        1 catagcagat cccctgggca ggcacattac accccacgac aacgcacgcg ttctggacgc
       61 aggcctggcc cggttcggta caaccgccgc aaacctgtcc gcagacagga tcgaccccgc
      121 agacaattcc ggtgcagtcc ggaacgcacg gctcgcaccg ctccaggtca tcccttgtca
      181 gaaggtctgc aagggcctgg tcgtacgcgg tctggggatc ctcatggcaa cacgaataac
      241 ccgtggttcc ggccactttt tccacaacgg tggcggcgaa ggacgcgggc tcatattcgc
      301 cggcctggtg tccgcaggaa tgaacattca actgacaagt gccgtggggc gtgtcctgat
      361 cggtgcattc gactgccagc caccagccgc cttcacgatt gcatacggtt gcttcggatt
      421 gtttgtccaa agggtcatag acatacttga gcctgtaatc gggcacgccg gcatcgatcc
      481 ccctttcgca gaccccgact tcggtaaaag gacacgccga atcgactgtt ccgccgaaaa
      541 agccggtaca gacgtccgaa aaggctgccc cgcgttccgg ggcaatcccg ccgccaagtt
      601 caagacacgg ggcggaaatc ccgggaagct ggcaggcaat cctgtccgcg ccgacggccg
      661 aacagtgggc cgatggatct tcaaagggat atgaaaccac cagttcggcc tgaagcagat
      721 cccgctcacg ctgggccttg gcctcggcga ccacgacggc ctcgccggaa tcacagtaaa
      781 ggccatggta ataatgaagc gtatagccgc caccgcctga ataggtgaac tttgccgaaa
      841 gcaggcggcc ctgggcggga cagagtcccc atactccgcc gtcctcgacg cagaattcct
      901 cgtggctgga caggaactct tcaggcggat cttcatagga agagtacgaa tcgacacaac
      961 gcccaagatc cgcgcgatag catccattgt tcacgcattg tcccaggaca cactgcgcgc
     1021 cgatgcagtc gccacagttc agggtgccgc aaaccgggtc aagcccgcag accgtcgcgg
     1081 aacagtcggg tatgcagggg ttgccggtat cgatttcgac gtcatccgca tccggatccg
     1141 cgtcggcttc ggcatccgtg gccgcatcag ggtcgacgcc atggtcgaag gcatccggaa
     1201 tgccgacatc cgcgtcgcct gttacgtccg gggcgtttcc atcaacggta tcggcgacgt
     1261 cgtccggggt ttcaacatca ggccgggtgt cctggcctgc atcctcgccg gagtcaacct
     1321 cgggatgcga aacactgtcc gtcgcgacat cgtcgatggc gtcgaaaatt tcgccatcac
     1381 ccccacagcc tgaagcaagt cccccaaaga aaaaaacaat cccgaccgca accagatacc
     1441 tgctgattga agaaaccatg atacccccct tttgtcggcg tttggctgaa ctgatttcaa
     1501 ctatacagca ggttgaccat tacagagcaa gaaaaccttc caccggcact aataatcctg
     1561 gagtcccagg tacggcgggc cgaaagcgaa caccggggga cggccgaacg gggcgttgac
     1621 cgttatcgtc cgcggatcct tgataggggc ggcatccggg atagaggcgg catccgggtt
     1681 ccacacgttt caccccacat catcttttcc tgcatgcccc ccctaccctt cgcgctcccg
     1741 ggaaccccga atcgtcaccc tgaggcaatc ggggatttca ccacgaaaca ggagtcggaa
     1801 ccagcctggt ttcctgcgtg gtgtcgccga tctgcccgtc ggaattccga ccccagcagt
     1861 acaggcgcga atccactatg gcgcaggtat ggcgtccacc ggcggtcacg gccgatgcac
     1921 cggacgggaa gaccttgtaa aaaacctgta tcccgccaat cgtgtcattg ccgatctgac
     1981 cgaagtcgtt gtgaccgaca cagaaaagct gattgaaact atccacatag cacgtgtgaa
     2041 aggcaccggc ggccggcacg ggaggatccg gcagactcat cagcgcctgc cagaatgccg
     2101 cagtcgcggg cgaggtccgg cacgatccgg ttgcgcaagc cgaattgcct tcccccgcaa
     2161 tctgtcccgt cgaatcactg ccccagcact ccatctgata cagatactga ccgtatccga
     2221 taccgacccg cccgagtgaa cacgtgtgtt gcccacccgc ggaaatccac tcggtcattc
     2281 cggtttcgct gacaagccag gtcggcttca gcaggttgtc agtattgccg acaccgagct
     2341 gaccagactg gttgtccccc cagcagaccg cattgttctg gaaagttatg gcg
//
LOCUS       DAOPPA010000486         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_48640_length_2374_cov_3.488141, whole genome shotgun sequence.
ACCESSION   DAOPPA010000486 DAOPPA010000000
VERSION     DAOPPA010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48640_length_2374_cov_3.488141"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PKH54_12340"
     CDS             <1..1363
                     /locus_tag="PKH54_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOD00727.1"
                     /translation="LLKSLLIGTLGASVLIVPAVALLSYFGYVPHGVWISVVAGLFAG
                     VIIGQATEYFTSDEYKPTKGIAKQCTMGPATAVIDGIAVGMYSTGIPVVTIVVAILVA
                     FWAPGGFKDMALGLYGIGFAAVGMLATLGITLATDAFGPIADNAGGNAEMSHLPEEVR
                     QRTDALDSLGNTTAATGKGFAIGSAALTAMALLAGYLEEIRVWLGRMAANASDGIVVQ
                     GPIAFFKGRTAEATPQAVEAAKTLGKTLIGTNEATMANFMAAYDVTLMNPAVLCGLFL
                     GAMMVLVFSAMTLKAVGRAAGSMVEEVRRQFKAFPGIMDGTQKPDYASCVAISTAGAQ
                     REMLVPSLMAIIVPILVGLFFGVAGVMGLLVGGLSTGFVLAIMLNNAGGAWDNAKKHI
                     ERGNFGGKKSDAHKAGVTCDTIGDPFKDTTGPSLNILIKLMTMVSVVFSGLIVALGGK
                     LFF"
     gene            complement(1469..>2374)
                     /locus_tag="PKH54_12345"
     CDS             complement(1469..>2374)
                     /locus_tag="PKH54_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00728.1"
                     /translation="EDGYDNRLAEINAGHFGNNLAWERARQAVRDIVDEHRPDIVAFQ
                     ELFFDPECDEICAELNGTPDHDAACDPETGVFACATWGGVDESFLTVRHALGPDYEIA
                     CAPNHNDNCIGVRKGFGSLAVMPDATATAGAWIGGLDGMPPPSLCSKGSRVATGVVTV
                     NNGPQIAVVDVHTVAGNNADCRVDQFYQVFVDRGDGKPATFGENNIVLGDMNIDPFLF
                     NDESVDFWNDHVGSDKPFHYISSGDASGPMTHPATFSRLDHVISDTMTGSCVVLGITD
                     GTDAPLSPTSTYFDHRPVLCTVNMP"
BASE COUNT          454 a          721 c          733 g          466 t
ORIGIN      
        1 ccttctgaag tcccttctga taggcacgct tggcgcgtcg gtcctgatcg tcccggcggt
       61 cgcccttctt tcatactttg gatacgtacc acatggcgtc tggatttcgg ttgtcgcagg
      121 cctgttcgcg ggcgtcatca tcggccaggc gaccgaatac ttcaccagcg acgaatacaa
      181 gcccacgaag ggcatcgcga agcagtgcac gatgggcccg gcaaccgcgg tcatcgacgg
      241 catcgcggtc ggcatgtatt ccaccggcat cccggttgtg acaatcgtcg ttgccatcct
      301 cgtggccttc tgggcaccgg gcggattcaa ggacatggcc cttggccttt acggcatcgg
      361 attcgcggcc gtcggcatgc tggcgacgct gggtatcacc ctggccaccg acgcctttgg
      421 cccgatcgcc gacaatgccg gtggaaacgc cgaaatgtcg cacctgccgg aggaagtcag
      481 gcagcgcacc gacgcgctgg acagccttgg aaacacgacc gcggccaccg gcaagggatt
      541 tgccatcggt tcggccgcgc tgaccgccat ggccctgctg gccggatatc tggaagaaat
      601 cagggtctgg ctgggacgca tggccgcgaa cgcatcggac ggcatcgttg tccagggccc
      661 gatcgccttc ttcaagggca ggaccgcaga ggcgactccc caggccgttg aggcggcaaa
      721 aacccttgga aagacactta tcggcaccaa cgaggccacc atggcaaact tcatggcggc
      781 atacgacgtg accctgatga acccggccgt cctgtgcggg ttgttccttg gggcgatgat
      841 ggttctggtg ttttccgcga tgacgctgaa ggcggtcgga cgcgcggccg gttcgatggt
      901 cgaggaagtc cgccggcagt tcaaggcatt ccccggaatc atggacggaa cccagaagcc
      961 ggactacgca agctgcgttg caatcagcac ggccggggcc cagcgcgaga tgctggtgcc
     1021 gtcactgatg gcaatcattg ttccaatcct tgtcggcctg ttcttcggcg tggccggcgt
     1081 catgggcctg ctggtcggtg gcctttcgac cggattcgtc ctggcgatca tgctgaacaa
     1141 cgcgggtggc gcgtgggaca acgcaaagaa gcacatcgaa cgcggcaatt tcggcggaaa
     1201 gaaatccgat gcccacaagg ccggcgttac gtgcgacacg atcggcgacc ccttcaagga
     1261 caccaccggg ccgtcgctta acatcctgat caagttgatg acgatggttt ccgtcgtatt
     1321 cagcggcctg atcgtcgcac tcggcggcaa gctcttcttc tgatttccac cgacaaatcc
     1381 agcatcactt ctgacagacg ggcgaatttt cacgcaggcg ggcgaatttt cattcgcccg
     1441 tacggatcgc ggggcggatc gggccgggtc acggcatatt gacggtgcac agcacggggc
     1501 ggtgatcgaa ataggtgctg gtcggcgaca aaggcgcgtc ggtgccgtcg gtgatcccca
     1561 ggacgacgca ggaaccggtc atcgtgtcgg aaataacatg gtcaaggcgt gaaaacgtcg
     1621 ccggatgggt catcggcccg ctggcatcgc ccgacgaaat gtaatggaaa ggcttgtcgg
     1681 aaccgacgtg gtcgttccag aaatcgacgc tttcgtcatt aaaaaggaac gggtcgatat
     1741 tcatgtcgcc aagcacgatg ttgttttcgc cgaacgtggc cggcttgccg tcgccacggt
     1801 caacgaacac ctgataaaac tgatccaccc tgcaatcggc gttgtttccg gcaacggtgt
     1861 ggacatcgac gaccgcgatc tgcgggccat tgttgacggt cacgacgccc gtggcgaccc
     1921 ttgatccctt gctgcaaagg cttggcggcg gcatgccgtc aagaccgccg atccaggcgc
     1981 cggccgttgc ggtcgcgtcc ggcattacgg ccagcgaacc aaaccccttc ctgaccccga
     2041 tgcagttgtc gttgtggttc ggcgcgcacg cgatttcata atcaggccca agggcatgcc
     2101 ggacggtcag gaacgattca tccacgccac cccatgtggc gcatgcaaac acgcccgttt
     2161 ccgggtcaca agcggcgtcg tgatccgggg tgccgttcag ttcggcacag atttcatcgc
     2221 actcggggtc aaagaacaat tcctggaagg cgacgatgtc cgggcgatgt tcatcaacga
     2281 tgtcccggac cgcctgtctg gccctttccc acgcaaggtt gttgccgaag tgtccggcgt
     2341 tgatttccgc cagccggttg tcgtatccgt cttc
//
LOCUS       DAOPPA010000487         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_48854_length_2367_cov_3.011678, whole genome shotgun sequence.
ACCESSION   DAOPPA010000487 DAOPPA010000000
VERSION     DAOPPA010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48854_length_2367_cov_3.011678"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..427
                     /locus_tag="PKH54_12350"
     CDS             122..427
                     /locus_tag="PKH54_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00729.1"
                     /translation="MDQKMMWCERCQVTVNARQGTNPMMKKIGLLVMALAVPEAAIFL
                     WFLPAGPIQFVGAAGIFISSIMSGLILIRMHAGFRCPVCGGPTPTERPAPRTDFTRV"
     gene            429..908
                     /locus_tag="PKH54_12355"
     CDS             429..908
                     /locus_tag="PKH54_12355"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOD00730.1"
                     /translation="MDKKVQTVKTIALVAHDNRKKDLVAWVEYNWQTLVRHKLVATGT
                     TGRLLEETICRKAGGEMPDCCKVLRLKSGPLGGDQELGAMIANGGVDILVFFWDPMAA
                     QPHDVDVKALLRIAVLYNVPTANNRSTADFLISSPLFERDYKPDVKDYSEYLSRIVS"
     gene            complement(989..1702)
                     /locus_tag="PKH54_12360"
     CDS             complement(989..1702)
                     /locus_tag="PKH54_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00731.1"
                     /translation="MNLSGFAFKLGILSLAGSFALSGLAGCSHIKQTQFETIGVGGPV
                     WVKRTVRTDEKTLFGSRMVDYEVGFLYCQVAENRGPVCVPVEVSDIPRGLTHQGIEFL
                     NGPAQISIHRKVITYQSLQADDDQEPTPPETNSVADERQIRSNAPEQRDMEASSTPQQ
                     FEVIYNVEGGWGDKELKLRLSSYIGKPIVLVSRTGKVFRGTLIEARLGQEGPGPRIVI
                     STNGTQSFLPLKAIKFIGK"
     gene            1725..>2367
                     /locus_tag="PKH54_12365"
     CDS             1725..>2367
                     /locus_tag="PKH54_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOD00732.1"
                     /translation="MVSCIIRDYRTLSIESSGLQIVIVRVGFTFVTVASYHRASFWLG
                     CCVDMEEERGGGLEDGGVESVSDDGTVGQGRSGKGRKDLERWSFLGFVALMTALFLWM
                     LKPFFGAIFWACALTVMFYPLQRRMLGWMRGRQNLAALATLAVMCSIVVVPAVFLIIS
                     FVNQGTDLYKYLSANGWAPARDMFEKVASLLPPVAAVNRARFSWTRTWDKDPRG"
BASE COUNT          468 a          617 c          737 g          545 t
ORIGIN      
        1 gacatgctgg tgggcacagg cgcgctcaag cggctggatg caggccatgc cgaatgtaat
       61 ggtatcaacg gaacgtggcg tgtaagagaa tagtcacgtc gggcgcacgg atggtgagac
      121 cgtggatcag aagatgatgt ggtgtgaaag gtgccaggtg acggtgaacg cccgccaggg
      181 caccaatccg atgatgaaga agataggcct tctggtcatg gcccttgccg tgccggaggc
      241 ggccatcttt ctgtggttcc ttccagccgg tccgattcag tttgtcggtg cggccggcat
      301 attcatttcc agcatcatgt caggcctgat cctgattcgc atgcatgccg gcttcagatg
      361 ccctgtctgc ggtggcccga ccccaaccga aaggccggca ccccgaaccg acttcacaag
      421 ggtttgacat ggataagaag gtgcagacgg tgaagacaat cgcgctggtg gcgcacgaca
      481 atcgcaagaa ggacctggtg gcctgggtcg aatacaactg gcagacgctt gtccggcaca
      541 agctggttgc gaccggcacg accggaaggc tgctggagga aacaatctgc aggaaggccg
      601 gcggggaaat gcccgactgc tgcaaggtcc tgcgcctgaa gtccggtccg cttggcgggg
      661 atcaggagct tggggccatg atagccaacg gcggcgttga tatactggta tttttctggg
      721 atcccatggc cgcccagccc catgacgtcg atgtgaaggc cctgctgcgg attgcggtgc
      781 tttacaatgt gccgaccgcg aacaatcgat ccactgccga cttcctgatc tccagcccgc
      841 tgtttgaacg cgattacaaa ccggacgtca aggattactc ggaatacctt tcaagaattg
      901 tgtcctgata gagcgcggtt tgccgtcaat gccgttcgtg cccgggtaat ctggcgggcc
      961 gcggccgtga tcagataagc caactcgatc acttgccgat gaacttgatg gccttcaggg
     1021 gaaggaatga ctgggttcca ttcgttgata tgacgatccg tggaccgggg ccttcctgcc
     1081 cgagtctggc ctcgatcaga gttcccctga aaacctttcc tgtcctggaa accagcacaa
     1141 tcggcttccc gatgtatgaa gacaggcgaa gtttcagttc cttgtcaccc catccgcctt
     1201 ccacgttgta aatgacttca aattgctgtg gcgtagaact ggcttccatg tccctttgtt
     1261 ccggggcgtt ggatcttatc tggcgttcat ccgcgacgga attcgtctct ggcggtgtcg
     1321 gttcctggtc atcgtcggcc tgaagcgact ggtatgtgat cactttccga tggattgata
     1381 tctgcgcggg accattaagg aactcgattc cctggtgggt gaggccgcgc ggaatgtcgc
     1441 tgacttcaac cggaacgcag actggccctc ggttttcggc aacctggcag taaaggaatc
     1501 cgacttcata atcgaccatg cgggaaccga agagagtctt ttcatcggtt cgcacggtgc
     1561 gcttgaccca taccgggccg ccgaccccga ttgtctcgaa ctgggtttgc ttgatgtgtg
     1621 agcatccggc aagaccggac aacgcaaagg aaccagccag cgacaggatg ccgagcttga
     1681 aggcaaatcc ggaaagattc atatatcccc catggaaggt aatgatggtg tcctgcatta
     1741 tccgtgacta ccggacgctg tcaatcgaat catccggtct ccagatcgtc attgttcggg
     1801 tcggtttcac gtttgtcact gttgcgtcgt atcatcgtgc ttcattctgg ctggggtgtt
     1861 gcgtggatat ggaagaagaa cgtggcgggg gcctggaaga cggcggtgtg gagtccgttt
     1921 cggacgatgg gacggtgggg caggggcgat ccggcaaagg ccgcaaagac ctggagcggt
     1981 ggtcgttcct tggtttcgtg gcgctgatga cggcactgtt cctgtggatg ctgaagccgt
     2041 tcttcggggc gattttctgg gcgtgcgccc tgactgtgat gttttatccc ctgcagcgcc
     2101 ggatgcttgg atggatgcgc gggcggcaga atctggcggc gcttgcgacc cttgcggtga
     2161 tgtgttcgat tgtggtcgtt ccggcagtgt tccttatcat ctcgttcgtc aatcagggga
     2221 cggatcttta caaatatctt tccgcgaatg gatgggcgcc agccagggat atgttcgaaa
     2281 aggttgcgtc gttgctgcca ccggttgcgg cagtaaaccg cgcccggttt tcttggacac
     2341 gaacttggga taaagatccc aggggag
//
LOCUS       DAOPPA010000488         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_49761_length_2335_cov_2.978070, whole genome shotgun sequence.
ACCESSION   DAOPPA010000488 DAOPPA010000000
VERSION     DAOPPA010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49761_length_2335_cov_2.978070"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKH54_12370"
     CDS             <1..1104
                     /locus_tag="PKH54_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00733.1"
                     /translation="IGTDLAGVSSDSFSCADCGNDGDCPSGWSCLSTDGTERKCARPC
                     NDGGDCPNGFFCGFLDGDLFCIPSTFNCVACMGDEPCGEGLCCDFMTGDCADCRGDCQ
                     TCTADYQCEAGSRCWKAGDSDLGVCTVECAAGGCPNPNFRCSVRADGVDVCEPDIDFC
                     SPCQGLCTADFPVCAIVRGVEQCVKCATNADCDALFPDDGCTCSGDPLYACIDLEGNL
                     CGVSTDGCISPCSVAGDCPPTADDSFLSCHVPDGGGAGTCYDPDGGCNALTACCGPGM
                     KCFDMTVFMEMISSGTLPRLIPHPVVDRSFCECDTTADCINGKTCTELSGYCDTTEYP
                     QEFVDMMCPGGVLHTALPPRLCGVLTEFLVGRE"
     gene            1127..>2335
                     /locus_tag="PKH54_12375"
     CDS             1127..>2335
                     /locus_tag="PKH54_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00734.1"
                     /translation="MSNRISVLLLLSCLTALPACGPTTAADILDLQPDAIADGVAIDI
                     HRDSDPDNAPTDPGVHDGAVDVSPSDSFPDPELELPDGGDDGSYTDGESENQDSRETD
                     VPDDDGPDAWFSCDICENDDECADGWACKAVRVEKFCVKGCMDDGECDPGFTCEKRDD
                     RSFCIPVTQNCHSCMADEPCPPGKCCDFITGYCMPCGGECAPCWYDFDCLSGYRCFNN
                     DDAGGGVCVPDCAGGTCEDTSKFECVIHENGVRLCEPFVQDCGGCHGDTPYLIDGECF
                     ECLADSDCLPTEECLQSGHACRKVKCPEPLQFCVDDRQCHQCCGQDSQCDYGDGSAGQ
                     CLQDRTCSNYPPCGGLCTVDFPVCTTVQGVEKCVQCATDNDCAMIGPDCHCSGDPLYS
                     CVDSEGLICTC"
BASE COUNT          425 a          675 c          701 g          534 t
ORIGIN      
        1 atcggcaccg acctcgccgg ggtttcgtcc gactcatttt cctgtgcgga ttgcggcaac
       61 gacggggact gtccttccgg atggtcctgc ctgtcaaccg atggcaccga acggaagtgt
      121 gcaagaccat gtaacgacgg cggcgactgc ccgaacggtt ttttctgcgg tttcctcgat
      181 ggcgatctgt tctgtattcc gtcaacgttc aactgcgttg cgtgcatggg cgatgaaccc
      241 tgcggcgaag ggctttgctg cgatttcatg accggcgatt gtgccgattg tcgtggcgat
      301 tgccagacct gcaccgccga ctaccagtgt gaagccggat cccgctgctg gaaggccggt
      361 gattcggatc ttggggtctg cacggtcgag tgcgctgccg gcggctgtcc gaacccgaat
      421 ttccgttgtt ccgttcgcgc cgacggcgtc gatgtctgcg agcccgatat agatttctgc
      481 tcgccgtgcc agggattgtg cacggccgat ttcccggtat gcgcgattgt ccgtggcgtc
      541 gagcagtgcg taaaatgcgc cacgaatgcc gattgcgacg cgttgttccc ggacgatggc
      601 tgcacatgca gcggggatcc cctttatgcc tgtattgatt tggaaggcaa tctctgcggg
      661 gtttccacgg acggatgtat ttccccatgc agcgtcgccg gggactgccc gcccaccgcg
      721 gacgacagct tcctgtcatg tcatgtgcca gatggcgggg gtgccggtac ctgctatgac
      781 cccgacggtg gctgcaatgc attaacagca tgctgcgggc cgggcatgaa gtgcttcgac
      841 atgacggttt tcatggaaat gatttcgtcg ggcacgctgc cacgcttgat tccccacccg
      901 gtcgttgatc ggtcgttctg cgaatgcgac acaaccgccg actgcatcaa cggcaagacg
      961 tgcacggaac tgtccggata ctgcgatacc acggagtatc cacaggaatt tgttgacatg
     1021 atgtgtcccg ggggcgtgtt gcataccgcc ctgccaccgc gcctctgcgg ggtactgacc
     1081 gagttccttg tcggccggga gtaatccttt gaatggggaa tccgccatgt cgaacagaat
     1141 atctgtattg cttcttctgt cctgcctgac ggcgctgccg gcctgcggcc cgaccaccgc
     1201 cgccgacatc ctcgacctgc aacctgacgc catcgccgat ggtgtcgcca ttgacatcca
     1261 tcgtgattcc gatccggaca acgcccccac cgaccctggc gttcacgatg gtgctgttga
     1321 tgtcagtcct tccgattcgt ttcccgatcc cgaattggaa ctgccagatg gcggtgacga
     1381 cggctcctac accgatggcg aatccgaaaa ccaggattcg cgcgaaacgg acgtccccga
     1441 cgatgacggg cctgatgcgt ggttctcatg cgacatctgt gaaaacgacg acgagtgtgc
     1501 cgacggatgg gcctgcaagg cagtccgtgt tgaaaaattc tgcgtcaagg gttgcatgga
     1561 cgacggcgaa tgcgaccccg gattcacgtg cgaaaagcgt gacgacagaa gtttctgcat
     1621 ccctgtaacg cagaactgtc attcctgcat ggctgacgaa ccgtgcccgc ccggaaagtg
     1681 ttgcgacttc attaccggat attgcatgcc gtgcggtggg gagtgtgccc cgtgctggta
     1741 cgattttgac tgtctctccg gttacaggtg tttcaacaac gatgatgccg gtggtggtgt
     1801 ctgtgtaccc gactgcgctg gcggcacctg tgaagatacg tcaaagttcg agtgcgtgat
     1861 tcatgaaaat ggcgttcgtc tgtgcgaacc cttcgttcag gactgcgggg gatgccatgg
     1921 tgacacgccg tacctgatcg atggggaatg cttcgagtgc cttgccgact ccgactgcct
     1981 gccaacggaa gagtgccttc agtccggcca cgcatgccgg aaagtcaaat gtcctgaacc
     2041 ccttcagttc tgcgtcgatg accgtcagtg ccatcaatgc tgcggacaag attcccagtg
     2101 cgactatggg gacgggtcag ccggccagtg tcttcaagat cgtacatgtt caaattatcc
     2161 tccgtgcggc ggtctctgca ccgttgattt cccggtatgc acgacggttc agggcgtcga
     2221 aaagtgcgtt cagtgcgcca ccgacaacga ttgcgcgatg attggcccgg actgccactg
     2281 ctcgggcgat cccctgtatt cctgtgtgga ttccgaaggt ctgatttgca cctgc
//
LOCUS       DAOPPA010000489         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_50081_length_2323_cov_3.573633, whole genome shotgun sequence.
ACCESSION   DAOPPA010000489 DAOPPA010000000
VERSION     DAOPPA010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50081_length_2323_cov_3.573633"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH54_12380"
     CDS             <1..181
                     /locus_tag="PKH54_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00735.1"
                     /translation="LCILSLGIGSLWIMPYHYGARLLFYRDLINQNLADRPIPENAPE
                     MIAGFYGTSNQNDTL"
     gene            268..>2323
                     /locus_tag="PKH54_12385"
     CDS             268..>2323
                     /locus_tag="PKH54_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00736.1"
                     /translation="MRYRHLLPICMISLTIFTACADDVTGIALTPEGDGPVVVWQQDP
                     ESGSPEVPFPNNFLTRREETSITGLRVNLPLNAKTQAEDKVLRLVDWMDGFGISSPIS
                     VSFSKHVDVVDLMARQADNHDYSDDAIILVNVTPGSENFGKPVPLDVGQGNFPLSLTW
                     PWQYWDFDPHADSINLMYETHDEDINNNGKLDEYEDIDFDGVLDKPNTFSGTDPGVNN
                     PDDLITFYERETKTLVLWPVAPMDPGATYAVIITNRLKGEDNKPVRSPFPYVNDASQT
                     EALTPAVDVLAGEPFNLALKDVAFAWTFTTQTISQELVAIRDGIYGHGPLASLKDEFP
                     INLTPDAIWEKDQDGNDAAANQHYRLPNDTVMDLFDTIGVILYDPAMVATLKADTKHV
                     DYWVLGEFEGPNFLVDRDGIATPMYPADDNESFFIDLVKGAAVVGPGKVSFMCAIPKA
                     TDQHKAPFPVMFYGHGFSSAVFEMFGFAGRMAQHGYALCATDAPGHGLALPVEKTTDW
                     PQLVDDLLTYSFPYLKPFYAALANGRVRDLDNDGQIASFDNGGDLWGWDVFHTRDTIR
                     QGVVDHMNFIRVLRGLGELKWDADVNGNGKADDLAGDFNGDGVVDIGTAKNIDYPVWG
                     QSMGAFNAIILAGVEPCVAAAAPVSGAGGMIQAGLRTINPGTAEGALLPMMGPFVVFT
                     PMP"
BASE COUNT          482 a          691 c          672 g          478 t
ORIGIN      
        1 gttgtgcatt ctgtctcttg gaatcggttc gctgtggatc atgccttacc attacggcgc
       61 caggcttctg ttttaccgtg atcttataaa ccagaacctg gcggatcgcc cgattcccga
      121 aaacgccccg gaaatgattg ccggctttta cgggacctcg aaccagaacg acaccctgtg
      181 attgactttg agcccccggt gattcaatct ttccgccatc agtggacccg ttggtccttt
      241 tcccaggtct ttttcaaggg acttaagatg cgatacagac accttcttcc aatctgcatg
      301 atttcactta ccatttttac ggcgtgcgcc gatgatgtca ctggtattgc cctgactccc
      361 gaaggagacg gaccggtggt cgtctggcag caggatcccg aatccggcag ccccgaggtg
      421 ccgttcccga acaatttcct tacccgccgt gaagaaactt ccattacggg ccttcgcgtg
      481 aacctgccgc ttaacgcaaa gacacaagcc gaggacaagg tgcttcgcct ggttgactgg
      541 atggacgggt tcggaatttc atcgccgatt tccgtttcgt tttcaaagca tgtcgatgtg
      601 gtcgatctga tggcacggca ggccgacaac cacgactatt cggacgatgc catcatcctg
      661 gtcaacgtaa cgccgggctc ggaaaacttc ggcaaaccgg ttcccctgga cgtgggccag
      721 ggcaacttcc cactttcgct gacgtggccc tggcagtact gggacttcga cccgcacgcg
      781 gactccatca acctgatgta cgagacgcat gatgaagaca tcaacaacaa cggaaagctg
      841 gacgaatacg aggacatcga tttcgacggc gtgcttgaca agccgaacac tttttccggt
      901 accgatccgg gcgtgaataa ccctgacgac ctgatcacgt tttacgaacg ggaaacgaag
      961 acgctggttc tctggccggt cgcgcccatg gatcccgggg ccacctatgc cgtcatcata
     1021 acgaacaggc tgaagggcga ggacaacaaa ccggtcaggt cgccgttccc gtacgtcaac
     1081 gacgccagcc agaccgaggc gctgacgccc gcggtcgatg tgctggcggg cgagccgttc
     1141 aatctggcgc tgaaggacgt ggcctttgcc tggactttca cgacccagac aatcagccag
     1201 gaacttgttg ccatccgtga cggcatttat ggccacgggc cgcttgcatc ccttaaggac
     1261 gaattcccga tcaacctgac gccggacgcc atctgggaaa aggatcagga tgggaacgac
     1321 gcggccgcca accagcatta caggctgccg aacgacacgg tgatggatct gttcgacacc
     1381 atcggtgtga tcctttacga cccagccatg gtcgccacgc tgaaggcgga tacgaagcac
     1441 gtcgattact gggttcttgg cgaattcgaa ggcccgaatt tccttgttga ccgcgacggg
     1501 attgccacgc cgatgtatcc ggccgacgac aacgaaagct tcttcatcga cctggtcaag
     1561 ggcgcggcgg ttgtcggccc aggcaaggtt tccttcatgt gtgccattcc aaaggccacg
     1621 gatcagcaca aggcgccatt ccccgtgatg ttttacggcc acggtttttc cagtgccgtt
     1681 ttcgaaatgt tcggctttgc gggcaggatg gcccagcacg gatatgcttt gtgcgcgacg
     1741 gacgcacccg gccacggact tgcgctgccg gtggaaaaga cgaccgactg gccacagctt
     1801 gtcgatgacc tgctgaccta ttcattccca tacctgaagc cgttttacgc ggccctggcg
     1861 aacggacgcg tgcgcgacct ggataacgac ggacaaattg ccagctttga caacggcggg
     1921 gatctgtggg gatgggacgt gtttcacacg cgtgacacca tccgccaggg cgtcgtcgat
     1981 cacatgaact tcatccgcgt cctgcgcggg cttggcgaac ttaagtggga tgccgacgtg
     2041 aacggtaacg ggaaggcgga cgacctggcc ggcgacttca acggagacgg cgtggtcgat
     2101 atcggcaccg cgaaaaacat cgattacccg gtctggggac aatcgatggg cgcgttcaac
     2161 gccatcatcc tggccggcgt cgagccatgc gtcgcggcgg ccgccccggt atccggggcc
     2221 ggcggcatga ttcaggccgg gcttcgcacg atcaacccgg gcacggcgga aggcgccctg
     2281 ctgccgatga tgggaccgtt cgtggtgttc acgcccatgc cgg
//
LOCUS       DAOPPA010000490         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_50476_length_2309_cov_3.474712, whole genome shotgun sequence.
ACCESSION   DAOPPA010000490 DAOPPA010000000
VERSION     DAOPPA010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50476_length_2309_cov_3.474712"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKH54_12390"
     CDS             complement(<1..315)
                     /locus_tag="PKH54_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD00737.1"
                     /translation="MKGFFLVVPLIVWMAFLDSASAQSNGGISVVSKPASAAVYVDDV
                     VRCKTTPCKVTKLSEGRHHIVVVKGTLVDETLIDVRAGKVHKYQAVLKRANPEIEISS
                     NKK"
     gene            complement(514..1434)
                     /locus_tag="PKH54_12395"
     CDS             complement(514..1434)
                     /locus_tag="PKH54_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOD00738.1"
                     /translation="MKICFIVNPNSANGQTRARFAAIQPGLAAVFPEMKVRYTERPCH
                     AIDLACEAAGQDFEAIVAVGGDGTLNEVVSGLDRCGRPDSVLLGMIPSGTGGDFRKTS
                     GFPKSMEQAVAYLSDWRERRVDYGVLTFRNLDGSSGRRPFINIASIGLSGVTDDYVNH
                     TTKAFGGKASFMIGAVRGILAYRNVDMTVRVDGVQIHEGRTALVTMANGRFFGGGMMM
                     APDAELDDGMLRVVILGDMGKLEFMSLSSAIYAGTHVKHPKCKLARGRVVEVEPASRA
                     LIDLDGEMPGMGPVRAEVIPGALRLMVPRL"
     gene            complement(1464..>2309)
                     /locus_tag="PKH54_12400"
     CDS             complement(1464..>2309)
                     /locus_tag="PKH54_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD00739.1"
                     /translation="VEQALFGLRRKVVEAGACPWPSARFVDNGQDLQQIYVDSVPPDY
                     VVPEASGVVFPGSGTLRTSPNLLKDLFECFGRLGVDTFAAVPSVADDSGYDLYAAGYI
                     AEFREHAGHVAKKLRRYKAVMVVSPTDYYAFKVLYPMHDIKLPDQVIFAEEHLGKLAL
                     ARQPGAKMTGRIAYHDSCITGRHLEIYDLPRRVIEHASGQPPIELRRHHQDSMCCGAS
                     GAWDIVNPKGSADAGANVARMACDAGANILVTSGCKCEANISSKLHGLEVMDLITFIA
                     RSTAL"
BASE COUNT          494 a          718 c          639 g          458 t
ORIGIN      
        1 ctttttattg ctggaaatct caatctccgg atttgcccgc ttgagaacag cttgatactt
       61 gtggaccttt ccagcacgaa catcaataag tgtctcgtca acaagagtcc cctttaccac
      121 aacgatgtga tgacgtcctt ctgacagctt ggttacctta caaggagttg tcttacatcg
      181 aacaacatca tcaacgtaaa ccgcagcgct tgcaggtttg cttacaacgc ttatgccccc
      241 attcgattgg gcggatgcgg aatcaaggaa cgccatccaa acaatgagcg gaacaaccag
      301 aaagaaaccc ttcatagacc atcctgacaa tatctagcgc gaacatgacc gagcttgtta
      361 ttggatcgga tagtatctgg tggggaaatg gatgtcaagc cggaatttac ctttagcgcc
      421 gccaccttag ttcgggaggt cggacggcgg gcgaattttc attcgccctt acggagcttg
      481 ggactatgca ggcgggcgaa ttttcattcg cacctacagg cgggggacca tcaggcggag
      541 ggcgccgggg atgacttcgg cgcggacggg gcccatgccg ggcatttcac catcaagatc
      601 gatcagggcc cggctggccg gctcgacttc gacgacgcgg ccgcgggcca gcttgcactt
      661 cgggtgcttc acgtgggttc cggcataaat ggcgctgctc agggacatga actcgagctt
      721 gcccatgtcg ccaagtatca ccacgcgcaa catcccgtcg tccagttccg cgtcgggcgc
      781 catcatcatt ccgccgccga aaaagcgacc gttcgccatt gtgaccagtg ccgtccggcc
      841 ttcgtggatc tgaacgccat cgacacgaac ggtcatatcg acgttgcggt aagcaaggat
      901 tccccggacc gcgccaatca tgaaggacgc cttgccgcca aaggccttgg tggtatggtt
      961 cacgtaatcg tcggtgaccc cggacaggcc gatgctggcg atattgatga atggtcgccg
     1021 gccggaactt ccatccaggt tcctgaatgt caggacgccg taatcgaccc ggcgttcacg
     1081 ccagtccgac agatacgcga ccgcctgttc catggacttc gggaatcccg acgtcttgcg
     1141 gaagtcgcca ccggtcccgc tgggaatcat tccgagcagg acggaatcgg ggcgaccgca
     1201 acggtcgagc ccgctgacga cctcgttcag cgtgccgtcg ccgccgaccg ccacgatagc
     1261 ttcaaaatcc tgcccggccg cctcgcacgc caggtcgatg gcgtgacacg gtcgttcggt
     1321 gtaacgcacc ttcatctcgg gaaacaccgc ggccagcccc ggctggattg cggcaaacct
     1381 ggccctggtc tggccattcg cactgtttgg attgacgatg aagcaaatct tcatggcagc
     1441 cccctcggcc ttcccccagg ccgtcacaac gcggttgatc tggcgataaa ggttatcaga
     1501 tccatgactt ccagcccgtg cagttttgaa ctgatattcg cctcgcactt gcagcccgac
     1561 gtcacgagaa tgtttgcacc agcgtcacag gccatcctgg ccacattcgc gccggcatcg
     1621 gccgatccct ttggattcac gatatcccag gcgccggaag caccgcagca catcgaatcc
     1681 tggtggtggc ggcgcaattc gattggaggc tggccggacg cgtgctcgat caccctccgc
     1741 ggaaggtcat aaatttccag gtggcgcccg gtgatgcacg aatcatgata ggcgatgcgt
     1801 ccggtcatct tcgcgccggg ctgtcgggca agagccagct tgccaaggtg ttcctcggca
     1861 aagatgacct gatccggcaa tttgatgtcg tgcatcgggt aaagcacctt gaacgcatag
     1921 taatcagtgg gcgacacgac catcaccgcc ttgtagcgac gcagtttctt cgccacatgc
     1981 ccggcgtgtt cacgaaactc agcgatgtaa ccggccgcgt aaaggtcata gcccgaatca
     2041 tccgcgaccg acggaaccgc ggcaaacgta tcgacaccaa gccgcccgaa acactcgaac
     2101 agatccttca gcaggtttgg actggttcgc agtgtcccgc tgcccgggaa taccacgccg
     2161 gacgcctcgg gcaccacata gtccggcggc accgaatcga cataaatctg ctgcaaatcc
     2221 tggccgttat caacgaaacg cgccgatggc cacgggcagg ctccggcttc aaccaccttg
     2281 cgccgcaacc cgaacagcgc ctgctcgac
//
LOCUS       DAOPPA010000491         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_50508_length_2308_cov_3.646693, whole genome shotgun sequence.
ACCESSION   DAOPPA010000491 DAOPPA010000000
VERSION     DAOPPA010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50508_length_2308_cov_3.646693"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2308
                     /locus_tag="PKH54_12405"
     CDS             <1..>2308
                     /locus_tag="PKH54_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00740.1"
                     /translation="PSPIDFGEINIGNSTTMDVSIRNVGTASFKIEQVMMSAEGSGAF
                     KLISIVPPEGDILPVDLDPDEVIGVTIEYTPQEGANEYSQLVVKGEENVWSFDVFGTG
                     LGPRLFINPKQIEIGGVDIGSTGRGLVVLKNVGNDSLRVTNVALSPGSHEGLAVEGDS
                     SFNLEPEDERDVYVAWTVTKAERATGQAIGWLQVASTDPASPAMVSIYGFVKSPLMTV
                     TPDNLDMGYGAQGITVFKSVAIRNDGSANLVIDGIPNLIEKTDEKYGQEWSVSTPDKV
                     PSGGKYTLAPGEGLEVKIGFTNKGPETGDIRAKLRIVSNDKSVPNTETLIPVKISRGG
                     TANCIPQLVPSAYDFGLVPMGFKKELPIRVVNTGLGYCSYKQTYIQDCSSGFGIGFGG
                     ACAEPFTTSKSTKFVVSQQPGVIQNGIGPGGSATIFVRFQPPQTGTMFGELTEYNAIM
                     GVMIYDSQLKKDLIVPKPSGTGTYSANLKGTSGVANLAVLPSEVKFGLVTLGCASKTY
                     QICAYNTGSAPLQVTGVKLDGCTPEFKLKGMPPLPRPITTGSPLCFGVNFVPQDTTED
                     SCILKVLSNEQSIPELFVGLSGQGTIYSDQTDVFTQLSGQEVDILFVIDDSGSMGDDQ
                     QTLIENFEYFINQAEVWQNDYHIGVVSTGLSYEHILGKLNLGNPRTLPRYITPGPNAK
                     SQFANLANLGSDGDNSEGALANAQAGLSAPLVTDTGVSCTRDEDCSSDPNLCTDAASC
                     PFKCLESTCGGWNKGFLREDAQLEIIALS"
BASE COUNT          494 a          681 c          680 g          453 t
ORIGIN      
        1 ccgagcccga tcgatttcgg cgaaatcaat atcggcaatt cgacgactat ggatgtctcg
       61 atccgcaacg tcggaacggc gtccttcaag atcgagcagg tcatgatgag cgcggaaggc
      121 tcaggtgcct tcaagctgat cagcatcgtc ccgcccgaag gcgacatcct tccggtcgat
      181 cttgatccgg acgaggtcat cggcgtcacc atcgaataca ccccgcagga aggcgcgaac
      241 gagtactccc aacttgttgt gaagggcgag gagaacgtct ggtcgttcga cgtgttcggc
      301 acaggccttg gaccgagact gttcatcaat ccgaagcaga ttgaaatcgg cggcgttgac
      361 atcggctcga ccggtcgcgg actggtggtg cttaagaatg tcggaaacga ctcgcttcgc
      421 gtgaccaatg tcgctttgtc gccgggaagt cacgaggggc tggccgtcga aggcgacagc
      481 agcttcaacc ttgagcccga ggacgaacgg gacgtttatg tcgcgtggac cgtcacgaag
      541 gcggaacggg cgaccggcca ggctatcggc tggcttcagg tggcaagcac ggatcccgcg
      601 tcccccgcga tggtctcgat ttacggtttc gtgaagtccc cgctgatgac ggttaccccg
      661 gacaacctgg acatgggata cggcgcccag ggtatcacgg tgttcaagtc ggtcgcgatt
      721 cgtaacgacg gttccgccaa ccttgtcatc gacgggatac cgaacctgat cgagaagacc
      781 gacgaaaagt acggccagga gtggagcgta tcgaccccgg acaaggttcc gagcggcggc
      841 aaatacacgc tggccccggg cgaagggctt gaggtcaaga tcggcttcac caacaaaggg
      901 cctgaaaccg gcgacattcg cgcgaaactt cgcattgtga gcaacgataa gtccgttccg
      961 aacaccgaaa cgctgatacc ggtgaagatc agccgcggcg gcactgccaa ctgcattccg
     1021 cagcttgtcc cgtcggcgta cgacttcggc cttgtcccga tgggcttcaa gaaggagctt
     1081 ccgattcgcg tcgtcaacac cggtcttggt tactgcagct acaagcagac ttatatccag
     1141 gactgctcat ccggattcgg catcggcttc ggcggtgcat gcgccgagcc tttcacgacg
     1201 tccaagtcca cgaaattcgt cgtttcccag cagccggggg tcattcagaa cggcatcggc
     1261 ccgggtgggt cagccaccat tttcgtcagg ttccagccgc cccagaccgg cacgatgttc
     1321 ggggaactca ccgaatacaa cgcgattatg ggcgtgatga tttatgactc acagctcaag
     1381 aaggatctga tcgttcccaa gccttcagga accggaacgt attccgccaa cctgaagggt
     1441 acgagcggcg tcgcgaacct ggcggtgctt ccttccgaag tcaagttcgg cctggtaacg
     1501 ctcggttgcg catccaagac ctatcagatc tgtgcctaca acaccggatc ggcaccgctg
     1561 caggttaccg gagtcaagct cgatggctgc acgcccgagt tcaagctgaa gggaatgccg
     1621 ccgctgccgc gtcccatcac gacaggctcg ccgctttgtt tcggcgtgaa tttcgttccg
     1681 caggatacca ccgaggattc gtgcatcctg aaagtccttt cgaatgagca gagcattccg
     1741 gagctgtttg tcggcctgag cgggcagggc acgatctaca gtgatcagac cgatgtgttc
     1801 acgcagcttt cgggacagga agttgacatc cttttcgtaa tcgacgactc cggttccatg
     1861 ggtgacgacc agcagacgct gatcgaaaac tttgaatact tcatcaacca ggcggaagtc
     1921 tggcagaacg attaccacat cggcgtcgtt tcgaccggcc tgagctatga acacatcctt
     1981 ggcaagttga accttggcaa tcccaggacg ctgcccaggt acatcacgcc gggcccgaac
     2041 gcgaaatccc agttcgcgaa cctcgccaac cttggcagtg acggcgacaa cagcgaaggc
     2101 gcgcttgcga acgcccaggc cggcttgtcg gcaccgttgg ttaccgatac cggcgtctcg
     2161 tgcacccgtg acgaggattg ttcttccgat ccgaacctgt gcaccgacgc ggccagttgt
     2221 ccgttcaagt gcctggaatc gacctgcggc ggctggaaca agggcttcct gcgggaggat
     2281 gctcagcttg agatcatcgc cctgtcgg
//
LOCUS       DAOPPA010000492         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_50684_length_2302_cov_3.757899, whole genome shotgun sequence.
ACCESSION   DAOPPA010000492 DAOPPA010000000
VERSION     DAOPPA010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50684_length_2302_cov_3.757899"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKH54_12410"
     CDS             <1..373
                     /locus_tag="PKH54_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00741.1"
                     /translation="TRADVVADGRKFDYGVYQGLHVSSVEIDFGTMKLFEYARKPGQL
                     HIVVGALPVHECAYKWPFYVVGKPIPGKQWHEFPGKSLDVVATTEQLLDPVIDGEMFF
                     GLDRLSVRLGPQLSFPVVKRH"
     gene            442..2166
                     /locus_tag="PKH54_12415"
     CDS             442..2166
                     /locus_tag="PKH54_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00742.1"
                     /translation="MIRRFCPIFAMLVFVCQAGCGSDGTDGEPDIVGVDVFFDSANPG
                     HDAAEHDASDGEEPPRSDAADADDGGSVDAWPDDGNVAQEAVLNVIIDDYKGVRNGLT
                     EARVLLYRKTAENRYDCLDLDARNLPSNVAVSREGVSIHGPVARFQELSGLAEDLEQE
                     YTVVALVEDGSDEPELLAWACNDRDAMVEYGAKVTVPLTLFDVAPSIVGSWDVSSSMD
                     FSRLLPPVARDVLDIVIDIMLRPSSSILMLMCDPSIAGIGGNEEFCAAIFSDPDNPDP
                     AAFTTSGQIAAQIIDSNHSSMLASACPYEEPALCEQVYFTVNELLSPLDEVRVLSTMT
                     CLGDPVSPYEIGDLMRAFGAQDCQENWHSVVFRWPLGHDCDPSDPECGAAYFSLGSIP
                     GIQTNLSCGISGSLGDGWHLNIEKHLVGLKPGALISFGLLSFILPSLLGDGSDGLPAV
                     NTVEKLIGSMLGGRACLTDSSCCDDFDSALDVGGAVMPVDSARAACESLIEQFSGLFV
                     QHFHDLDTGGGIMIATSAEGALLVDANGDQAFDLIGAREAPAAWLLEATVGQMSYEGS
                     GSFFGVEN"
BASE COUNT          427 a          613 c          685 g          577 t
ORIGIN      
        1 aaccagggcc gatgtcgtcg cggacgggcg caagttcgac tacggcgtgt accaggggct
       61 tcatgtgagt tcggtcgaga tcgatttcgg caccatgaag ttgttcgagt acgccaggaa
      121 gccggggcag cttcacatag tcgtcggcgc gcttccggtg catgaatgtg catacaagtg
      181 gccattctat gtcgttggca agccgatccc gggcaagcag tggcatgagt ttccgggcaa
      241 gtcgcttgat gtcgtcgcaa ctactgaaca gcttcttgat ccggtaatcg acggcgagat
      301 gttcttcggc ctcgatcgcc tgtccgtccg cctggggccg cagttgtcct ttccggtcgt
      361 caagcgccat tgatgatgtc cgttgaactt gccccctgtt cctgatactt tcgaattcag
      421 tcttatcctg tggatgtcat tatgattcga agattctgtc cgatttttgc aatgctcgtg
      481 tttgtttgcc aggctggttg cgggtcggac gggacggatg gggaaccgga catcgtcggc
      541 gttgatgttt tcttcgattc cgcgaatccc ggacatgacg cggcggagca tgacgcatcc
      601 gatggcgaag agccaccgcg ttccgatgcg gctgacgcgg atgacggcgg ttccgtcgat
      661 gcatggcctg atgacggcaa tgtcgcccag gaagctgtgc tgaacgttat catcgacgac
      721 tacaaaggcg ttcggaatgg cttgaccgaa gcccgggttc tgctctaccg gaagacggcc
      781 gaaaatcgat atgactgcct tgaccttgat gccaggaatc tgccgtcgaa tgtggcggtt
      841 tcccgcgaag gcgtttccat ccatggcccc gtggccaggt tccaggaatt atcggggctg
      901 gcagaagact tggagcagga atacacggtt gttgcgcttg tcgaggatgg atccgatgaa
      961 cctgaacttc ttgcttgggc atgcaatgac cgggatgcga tggtcgaata cggagccaaa
     1021 gttacagtcc ccctgaccct tttcgatgtt gctccgtcga ttgtcggaag ctgggatgta
     1081 agcagttcaa tggacttttc acgccttctg cctcccgtgg ccagggacgt actggatatc
     1141 gtgattgata tcatgcttcg tccgtcttca agcatactca tgctgatgtg tgatccgtcg
     1201 attgccggaa tcggcggcaa cgaagaattc tgtgcggcca ttttttccga cccggataat
     1261 cccgaccccg ccgccttcac gacatccggc cagatcgcgg cccagatcat cgattcaaac
     1321 cattcgtcca tgctggcttc ggcctgcccg tacgaggagc cagccctttg cgagcaggtc
     1381 tatttcacgg tcaacgaact gttatccccc ctggatgagg ttcgagtcct ttcaacgatg
     1441 acatgtctgg gggatccggt ctctccgtat gagatcggtg atcttatgcg ggctttcggg
     1501 gcgcaggact gccaggagaa ctggcattcg gtcgtctttc ggtggccgct gggacacgac
     1561 tgtgatccgt cggatcccga atgtggtgcg gcctatttca gccttggcag tattccgggc
     1621 attcagacga atctgtcgtg tggcatttcc ggcagccttg gcgacggttg gcatttgaac
     1681 atcgaaaaac atcttgtcgg gttgaagcct ggtgccctga tttccttcgg cctgctgtcc
     1741 ttcatcctgc cgagcctgct tggcgacggg tcggatggat tgccagccgt caataccgtc
     1801 gagaaactta tcggctcgat gctgggcgga agggcctgcc tgacagattc ttcctgttgc
     1861 gatgatttcg attcggctct tgacgtcggg ggcgcggtaa tgccggtcga ttcagcccgt
     1921 gccgcgtgtg aaagcttgat tgaacagttt tcaggtttgt tcgtacaaca cttccatgac
     1981 ctcgacaccg gtggcggcat catgattgca acctcggctg aaggtgcctt actggtggac
     2041 gctaacggcg accaggcttt tgaccttatc ggagcgcgcg aggctcctgc ggcctggttg
     2101 cttgaagcga ccgtcggtca gatgtcatat gaaggttccg ggtcgttttt cggtgtggaa
     2161 aattgatact gcacgcggag ttccgtcttc ccgattgacc atcgcctgcc ttccgttgta
     2221 ataacggaca tcaaccagaa cggtgcatga tgacccgcag tgaattgatg cggcatgtgc
     2281 aggaatcctg tcgcgagtcc ga
//
LOCUS       DAOPPA010000493         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_50848_length_2296_cov_4.892905, whole genome shotgun sequence.
ACCESSION   DAOPPA010000493 DAOPPA010000000
VERSION     DAOPPA010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50848_length_2296_cov_4.892905"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKH54_12420"
     CDS             <1..468
                     /locus_tag="PKH54_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00743.1"
                     /translation="RMFVTWCGDSRIYRFRAGVLSQVSEDHSLVNQYLKSGALTPEQA
                     RRFPHRNVILQALGLAEDFRYDWLQETVMSGDVFLLCSDGLNDMLTDDQIRDILSTAL
                     PSCGTDTGEDGAERDSNLLSEACHALVSAAMDHGGLDNITVMLLCVDPCEGQD"
     gene            580..1788
                     /locus_tag="PKH54_12425"
     CDS             580..1788
                     /locus_tag="PKH54_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD00744.1"
                     /translation="MTCNGFDGERCAAISRMIVGTGVLAIAVLLAAVVCMLQPAYRNW
                     KAVADQQQTRTELSAKYVAAGDASFAARDFQAAQTSYIMAASINPMDSLLSSMVARAR
                     IATFASNPALLDKADLQQARAECAIALDRFPTDMSNVKIVQGMISFRENRVNEALALL
                     EEAAAADPASAAVHVARAMLWTFEPTRVESVPAEWEAAVAAAPEDPMILGMAGQSLIN
                     SQETFEKGLDMLRKAVVTVRNLSWLKTIGSALVQSGKADEAVDYINQAYTLSPRDPEA
                     LALIGQIHIAKREWEKAIPPLRDSMSIRETRDVMLPLAMALNSTGRFDQALPILQKLN
                     QQGSDMMSVYEYANALAGVGRKEEAVNLYNAILGLSEQGLEGMQAQAVTTLKENARMA
                     VGRLTAAAPK"
     gene            1822..2241
                     /locus_tag="PKH54_12430"
     CDS             1822..2241
                     /locus_tag="PKH54_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD00745.1"
                     /translation="MHSGEPDRRMITEINVTPMVDIMLVLLIIFMVTASYITRGAFDV
                     NLPSAANSSDVGDSPIQISISAGGELSFNGQRSDIAGISQAVAPIIRDNPDVQAVVDG
                     DRGVEYGRVMEVIDALKTAGVRNFAAAVERVPAPASP"
BASE COUNT          464 a          646 c          710 g          476 t
ORIGIN      
        1 aggatgttcg tgacctggtg cggcgattcc aggatctacc ggttcagggc cggggttctg
       61 tcacaggttt cggaagacca ctccctggtc aatcagtacc tgaagtccgg cgcccttacg
      121 ccggagcagg cacggcgttt tccacacagg aacgtgattt tgcaggcgct tggacttgcc
      181 gaggatttcc ggtacgactg gctgcaggaa acggtgatgt ccggtgacgt cttccttctc
      241 tgttccgacg gcctgaacga catgctgacc gacgatcaga ttcgggacat cctttcaacc
      301 gcgcttccgt cgtgcggaac cgacacgggg gaggatggcg ctgaaaggga ctcgaacctg
      361 ctttcagagg cgtgccatgc cctggtatcg gcggcgatgg atcacggtgg ccttgacaac
      421 atcacggtga tgcttctgtg cgtggatccc tgtgaaggcc aggactgaac gcggccctga
      481 ttacccggac acgccccgcg ggccccagcc tttcaatcct gtcggcgttg gaacataata
      541 ggcaccggtt ttgtttaacc ggttaacgga gggggaataa tgacctgtaa cggttttgat
      601 ggagaaagat gcgctgcgat cagcaggatg attgttggaa cgggtgtcct ggcgattgca
      661 gtcctgctgg ctgccgtcgt atgcatgctt cagccggctt acagaaactg gaaggcggtc
      721 gcggatcagc agcagacccg caccgagctt tcggcgaaat atgtggcggc cggtgacgcg
      781 tcctttgctg cgcgtgattt ccaggccgcc cagaccagct acatcatggc tgccagcata
      841 aatccaatgg attcccttct gagttccatg gtggctcgcg cgcggattgc gaccttcgcc
      901 tccaatcctg ctcttctgga caaggccgac ctgcagcagg caagggcgga atgtgcgatc
      961 gccctggatc gcttcccgac cgacatgtcc aatgtgaaga ttgtgcaggg catgatcagt
     1021 tttcgcgaaa accgcgtgaa cgaggcgctg gccctgctcg aggaagcggc ggctgccgac
     1081 ccggcaagtg ccgccgtcca tgtcgcgcgg gcgatgttgt ggacgtttga accaaccagg
     1141 gtggaatccg tgccggccga atgggaagcc gcggtcgccg cggccccgga ggatccgatg
     1201 attctgggaa tggcaggcca gagcctgatc aattctcagg agactttcga aaagggcctg
     1261 gacatgcttc gaaaggcggt cgttacagtc cgcaaccttt cctggctgaa gaccatcggt
     1321 tccgcgctgg tgcagtccgg aaaggccgac gaggccgttg attacatcaa ccaggcatat
     1381 acgctgtcgc cgcgtgaccc tgaggcgctg gctctgatcg gccagattca cattgccaag
     1441 cgtgaatggg aaaaggcgat tcctccgttg cgggactcca tgtccatccg ggaaaccagg
     1501 gacgtcatgc ttccactcgc aatggcactg aattcaaccg gccgtttcga tcaggcgctc
     1561 cccatacttc agaagctgaa ccagcaggga agcgacatga tgagcgtgta tgaatacgcc
     1621 aatgcccttg ccggcgtcgg cagaaaggaa gaggccgtca acctgtacaa tgccattctg
     1681 ggattgtccg aacagggcct ggagggcatg caggcccagg ccgtcacgac cctgaaggaa
     1741 aacgcaagga tggccgtcgg gcgccttaca gccgcagcac cgaagtaggc cgggttcgaa
     1801 gtccctttac gggaggttgt tgtgcattcg ggtgaaccgg accgcaggat gatcaccgag
     1861 atcaacgtga ccccgatggt cgatatcatg ctggttctgc tgatcatttt catggttacg
     1921 gcaagttaca tcaccagggg tgcctttgac gtcaatctgc cgtccgcggc caattcgtcc
     1981 gatgtgggcg attcgccaat ccagataagc atttccgccg gtggggagct ttcattcaac
     2041 ggccagcgat ccgacattgc ggggatttcg caggctgtcg cgccgataat tcgcgataat
     2101 cccgacgttc aggccgttgt ggatggcgat cgtggtgtcg aatacggacg ggtcatggag
     2161 gtaatcgacg cccttaaaac ggccggcgtc cgtaatttcg ccgcagccgt ggaaagggtt
     2221 cctgctccgg cctctccttg atagcgggga ttcccatgcg tcccgtcggc aaccgcagaa
     2281 tcatcggaag aacgtc
//
LOCUS       DAOPPA010000494         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_51447_length_2276_cov_3.653309, whole genome shotgun sequence.
ACCESSION   DAOPPA010000494 DAOPPA010000000
VERSION     DAOPPA010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51447_length_2276_cov_3.653309"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="PKH54_12435"
     CDS             <1..2174
                     /locus_tag="PKH54_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00746.1"
                     /translation="LRVGQRTELEEMLQASSDVALPGDAALKRARAAFFRVVRGVRTI
                     SFKDKGKERADLVNYIRQEARRGTASVLAREALERRGASFAQTALLAACLAGEDKMLD
                     QAIDRLVPTSIGATDMPGGETAAAVDVLTVADLLFQCGRWRSAVEAAVGYLQMFDLDV
                     NDMNRAVHIAVKGALLARIPNRVTPDFIAKITDDQLMRFSLESAVARAAGDPESDVRA
                     ASVGLSLMKEQSSLFLDSTLASIRAGQVDGLDARVWSITESLPFPGRHVPVVVATLLD
                     FFQPRLARDFVLRAGDRYGWTIDRLSELFKARVETGDEAGAIETAGRILAMHADQTRS
                     ATVLLAYAAASLRYRVAFHLADGLLALNGGKVAGGLPPDVFDQVASSVQSFYRIDPAC
                     DRGARLLDFALLNARDREGACGQLVGRAFGAGPGDSGFIQAVQATFLDGRCSAAFLKD
                     QLADLLDARASGPKSVKFRNSYPDEGWMKLMNQAVRAAMIEGRIRDALDWCELGFGRL
                     GSVSDRVTLVQAVIEYIGDQTSVWSQDRAIAADFALRAIDVPGTDPTRFILLYSQILG
                     MADTEGNGAAVFGVMENEIGISPSSPTNRNNLAYTLSIFGHGKTRALDQIRVASELGG
                     MDRGAYLETEAWALFKYGQLHKALQIQLQARRFWSRSDSGLGLAECFNHLGTIQLALG
                     LRADATESFRLAVANGENWGWHSILSMRRLDELGFFEVGNR"
BASE COUNT          390 a          666 c          739 g          481 t
ORIGIN      
        1 cgcttcgcgt cggacagcgc accgaactcg aggaaatgct gcaggcgtcc tcggatgtcg
       61 cactgccggg cgacgccgcg cttaagcgcg ccagggcggc cttcttcagg gtcgttcggg
      121 gggtgcgcac cataagcttc aaggataagg gcaaggaacg ggcggatctt gtcaactata
      181 ttcggcagga ggcaaggcgc ggcaccgcat ccgtgcttgc gcgcgaggcg cttgaacgcc
      241 gcggggcatc gttcgcgcag accgccttgc tggccgcatg cctggccggt gaggacaaga
      301 tgcttgacca ggcgattgac aggctggtgc cgacctcgat cggggccacc gacatgcccg
      361 gcggcgagac cgcggcggcc gttgacgtgc ttaccgtcgc cgatcttctt ttccagtgtg
      421 gccgctggcg ttctgctgtc gaggccgcgg tcggctatct ccagatgttc gacctggacg
      481 tcaacgacat gaaccgggcc gttcacatcg ctgtaaaggg cgcattgctg gcaaggattc
      541 ccaaccgcgt aacccccgac ttcatcgcga aaatcaccga tgaccaactg atgcgttttt
      601 cgctggaatc cgccgtggct cgtgccgccg gggatccgga gtccgacgtc agggccgctt
      661 ccgtcggtct gtccttgatg aaggaacagt cctcgctttt cctggattcc acgcttgcgt
      721 caatcagggc ggggcaggtt gacgggctcg atgcgcgagt ctggagcatc accgaatcgc
      781 ttccgttccc cggtcgtcac gttccggtcg ttgtcgcaac cttgctggac ttcttccaac
      841 cccgcctggc cagggatttc gtgcttcggg ccggggacag gtacggatgg accatcgaca
      901 ggctgtccga gcttttcaag gcacgcgtcg agaccggcga cgaggccggg gcaatcgaaa
      961 cggccgggcg gatcctggcc atgcacgccg atcaaaccag gtcggccacg gtccttctgg
     1021 catacgctgc cgcgtccctt cgataccggg tcgccttcca ccttgccgac gggctccttg
     1081 ccttgaacgg gggaaaggtc gcgggcgggc ttcctcccga cgtattcgat caggttgcct
     1141 cgtcagtcca gtcgttttac aggattgatc cggcatgcga tcgcggggcc aggctgctgg
     1201 acttcgcgct tctcaacgcg agggatcgcg agggcgcctg cgggcagctt gtcgggcggg
     1261 ccttcggtgc cgggccgggc gattccgggt ttatccaggc agttcaggca actttcctgg
     1321 atggacgttg ctcggccgca tttctgaagg atcagttggc cgacctgctc gatgccaggg
     1381 cttccgggcc gaagtccgtc aagttccgga attcttatcc cgacgaagga tggatgaaac
     1441 tgatgaacca ggccgttcgg gccgccatga ttgaaggcag gatccgggac gcgcttgact
     1501 ggtgtgaact cgggttcggg cggcttggat ccgtcagcga ccgggtgacg cttgtacagg
     1561 ctgtgattga atacatcggg gatcagacgt cggtctggag ccaggatcgc gctattgccg
     1621 cggatttcgc gcttcgggcg atcgatgtcc ccggcacgga tccgacacgg ttcattctcc
     1681 tgtactcgca gattcttgga atggcggaca ccgaagggaa tggggcggcc gtctttggcg
     1741 tgatggaaaa cgaaatcggg atttcgccgt cgtcgcccac caaccgtaat aaccttgcat
     1801 acacgctttc gattttcggc catgggaaga cgagggcact cgaccagatt cgagtggcat
     1861 cggagttggg cggtatggat cgcggggcat accttgagac cgaggcttgg gccctgttca
     1921 agtacggcca gctccataag gcccttcaga ttcagttgca ggccaggcgt ttctggagtc
     1981 gaagcgattc cggcctgggc cttgccgaat gcttcaatca tctggggaca atccagcttg
     2041 cactcgggct gcgcgccgac gcgaccgaga gtttcaggct ggccgtggcc aacggtgaaa
     2101 actggggctg gcattccatt ctttccatga gaaggcttga cgaacttgga ttcttcgagg
     2161 tgggtaacag atgattgtca aagtggtaac gggcgtgatc ctttcggcgg ccattattgc
     2221 cgccattgtt cttgcgccat ctccggttct ggccggaatc atcgccgtga tcttcg
//
LOCUS       DAOPPA010000495         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_51451_length_2276_cov_3.031968, whole genome shotgun sequence.
ACCESSION   DAOPPA010000495 DAOPPA010000000
VERSION     DAOPPA010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51451_length_2276_cov_3.031968"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKH54_12440"
     CDS             <1..284
                     /locus_tag="PKH54_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD00747.1"
                     /translation="SGLSIITAVHSYEQFAAGLGNAVLVVFIMRTCNPEFKAAHYAIA
                     SAIASVGGTAFGGFGGLFVESFGYLNLYILAFAATIPGMIFLKLMKIRD"
     gene            409..1575
                     /locus_tag="PKH54_12445"
     CDS             409..1575
                     /locus_tag="PKH54_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD00748.1"
                     /translation="MTDNTLQKAIPGSRWFSLPVIFAIVITNGLLLSSCTKKVGGPRG
                     AISVDAELARKSDVNITLRAVGRVEPLQKVEIRPQVGGQIQEIRFAEGDAIEAGQTLY
                     VLDRRPFEAARAQAAAQVKASMIKQDAAKRDLERGRTLFERQMISAGELDALQAAADS
                     ADAAVQMARALERTSILNLEYAVIKAPISGRAGRHLVYPGDVVVPAVTPLVTINQTTP
                     ISVAFSVPERELAAVRAAASAGAVSVTATVSDGTGTPRSGTLNFIDNQVDPGTGSVTM
                     KAVFDNADESLWPGQFVQVGAQIGRETDAIVVVSKAVQVGQSGSFVFKLEGGNTARLA
                     PVKTGRTSGNDIVILEGVAEGDLVVTDGQLKLADGSKVEVRGIGRPATGQEAGQ"
     gene            1572..>2276
                     /locus_tag="PKH54_12450"
     CDS             1572..>2276
                     /locus_tag="PKH54_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD00749.1"
                     /translation="MNLNESGRQSFSGIFIRRPVATITLMAAILVVGIGGFVNLSVNN
                     LPTVDFPTIQVSASLPGANPETMASSVAAPLEKQFSAIQGIESITSTSFPGGTRITLQ
                     FQLERDIDAAAQDVQTAISAAIRQLPRDMQMPSFWKANPADMPIVMYALTSNTLPLST
                     LDEYGQTLLAQNISMVSGVAQVDVRGSQKYAVRIQLDPARMAAMGLSVTDVAAAVKRQ
                     NQKLATGTLWGDQKAVS"
BASE COUNT          485 a          670 c          700 g          421 t
ORIGIN      
        1 catcaggcct gtcgataatc accgcggttc acagctatga gcagttcgca gccgggctgg
       61 ggaacgcggt gcttgtggtc ttcatcatgc gcacctgcaa ccccgaattc aaggcggccc
      121 actacgcaat cgcaagcgca atcgcatcgg tcggcggaac ggcattcggg ggattcggcg
      181 gccttttcgt cgaatctttc ggctacctga acctttacat ccttgccttc gccgcgacaa
      241 taccggggat gatcttcctg aaattgatga aaatcaggga ttaattccag ggaactgcaa
      301 ccactgggaa ctccggggat tgacacggga ctgcgtgtgt cggttaatct ttgccgatcc
      361 cgggccgtgg caactgcctg atccgaatcc accctggagg ccgagccgat gacagacaac
      421 acccttcaga aggcaatccc gggaagccga tggttcagcc tgccggttat ctttgcgatt
      481 gtcatcacga acggcctgct gctttcatcg tgcacgaaga aggtcggcgg ccccaggggc
      541 gccatttccg tcgatgccga actggccagg aaatccgatg tcaacataac cctgcgggcc
      601 gtcggccggg tcgagccatt gcagaaggtc gaaatccgac cacaggtcgg gggacaaatc
      661 caggagatcc gctttgccga aggcgatgcc atcgaggccg gacagacgct ttatgtgttg
      721 gatcggcgcc cgttcgaggc cgcgcgagcg caggccgccg cccaggtcaa ggcttcaatg
      781 atcaagcagg acgctgcaaa acgcgatctt gaacgcggac ggactctttt cgaaaggcag
      841 atgatcagcg ccggtgaact tgacgcgctg caggcggcgg cggattcggc ggatgcggcg
      901 gtacaaatgg ccagggctct tgaaaggact tcgatactca atctggagta cgccgtcatc
      961 aaggctccca tatcagggcg tgcgggacgt cacctggttt atcccgggga tgtcgttgtc
     1021 ccggccgtga ccccgcttgt gaccattaac cagacaacac cgatcagcgt tgcgttttcc
     1081 gttcccgaaa gggaactggc cgcggtgagg gcggctgcgt cggccggcgc cgtcagcgtg
     1141 acagccaccg tgtcggacgg aaccggcacg ccaagatccg gaaccctgaa cttcatcgac
     1201 aaccaggtcg accctggaac cggaagcgtc acgatgaaag ccgtattcga caacgcggac
     1261 gaaagcctgt ggcccggcca gttcgtgcag gtcggcgcgc agatcggccg ggagacggat
     1321 gcaatcgtgg tcgtttccaa ggccgtgcag gtcggccagt cgggatcgtt cgtcttcaag
     1381 ttggaaggtg gaaacaccgc acgacttgcc cctgtaaaaa ccggaaggac atccgggaac
     1441 gacatcgtga tccttgaagg tgtcgccgaa ggtgatctgg tcgtcaccga cggccagttg
     1501 aagctggctg acgggtcaaa ggtcgaggta cgcgggatcg gccggccggc aaccgggcaa
     1561 gaggccggcc aatgaacttg aacgaatcgg gccgacagtc gttttccggg atcttcatca
     1621 ggcgtccagt cgccacgatt acgctgatgg cagccatcct ggttgtcgga atcggcgggt
     1681 tcgtgaacct ttccgtcaac aacctgccga cggtcgattt cccgacaatc caggtcagcg
     1741 ccagtcttcc aggggcaaac cccgaaacga tggcttcctc ggtcgcggca ccgcttgaaa
     1801 agcagttctc ggccatccag ggaatcgaat cgatcacttc gaccagtttc cctggcggaa
     1861 ccaggatcac cctgcagttt caacttgaac gcgacatcga cgctgcggcg caggacgtgc
     1921 agacggcaat atcggccgcg atccggcaac ttccacgcga catgcagatg cccagcttct
     1981 ggaaggcgaa tccggccgac atgccgatcg tgatgtacgc gctgacgtcc aataccctgc
     2041 cgctttccac gctggacgag tatggccaga cgctgctggc ccagaatatc tcgatggtgt
     2101 ccggagtggc gcaggttgac gtccggggct cgcagaaata cgcggtcagg atccagcttg
     2161 atcccgcaag aatggccgcg atgggcttaa gtgtcaccga tgtagcggcc gcggtgaaaa
     2221 gacagaacca gaagcttgcg accggaacct tgtggggcga ccagaaggcc gtttca
//
LOCUS       DAOPPA010000496         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_51465_length_2275_cov_4.404955, whole genome shotgun sequence.
ACCESSION   DAOPPA010000496 DAOPPA010000000
VERSION     DAOPPA010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51465_length_2275_cov_4.404955"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKH54_12455"
     CDS             complement(<1..474)
                     /locus_tag="PKH54_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOD00750.1"
                     /translation="MAKFILALDEGTTGSTAMVVAQDLTFLAKAANEIRQIYPKPGWV
                     EHDPEDIWNATAMSARQAMEKAGVQPADCAAIAITNQRETSVIWDRDTGKARFNAIVW
                     QCRRTTAFCEKAKEEGLEQYFRNRTGLVLDPYFSGTKVRWMLDNVPGLRADAEAGK"
     gene            complement(786..1910)
                     /locus_tag="PKH54_12460"
     CDS             complement(786..1910)
                     /locus_tag="PKH54_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019593079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD00751.1"
                     /translation="MKKEWVWQSERWPDVPWDVERVGPPLDRARSRLEVLVASLTGLG
                     FCARLADQVRGTAIEVVRTAEIEGERLDLDSVRSSVARRLGLEVDSMDVTPAPGQAEG
                     LVDVLVDATMNAALPLTRERILGWHASLFPTGWSGMRRITPGSFRPADMTMQVVSGPL
                     GRETVHFVAPPGDSLDSEIDLFLRWFETPPGEMDAFVVAALAHFRFVTIHPFEDGNGR
                     LARAIADMALARGSGYPGRHFSMSAAIMKDRPRYYDELRRASAYDADLTAWIVWFLGC
                     LTSAVDEAGEQVAVALQKARFWQDHGGKTINVRQAKILNRLLDAGSGGFKGGMTTRKA
                     AHLTGAGTATAFRDLVDLVDKGMLIQNPGGGRSTSYRLNI"
BASE COUNT          501 a          708 c          605 g          461 t
ORIGIN      
        1 cttgccggcc tcggcgtcgg cgcgcaggcc ggggacgttg tccagcatcc agcgcacctt
       61 ggtgcccgaa aaatagggat ccagcacaag cccggtacgg ttccggaagt actgttcaag
      121 cccctcttcc tttgccttct cgcagaacgc cgtggtgcgg cggcactgcc agacaatcgc
      181 gttgaaacgg gcctttccgg tgtcgcgatc ccagatgacc gaagtctcgc gctggttggt
      241 gatggcaatt gcggcacaat cggcaggctg gacgccggcc ttttccatcg cctgcctggc
      301 cgacatcgcc gtggcgttcc agatgtcttc cgggtcatgt tcgacccaac cgggtttcgg
      361 atatatctgc ctgatttcat tggcggcctt cgccaggaac gtcaaatcct gcgccacgac
      421 catcgccgtc gaaccggttg tgccttcatc gagcgccaga atgaatttcg ccatcaccaa
      481 tctccccctt ccaatacatc aaactcgcgg ccgggaccgc tgacgatgcc cggacaacac
      541 acagggcgca gtttatcatc gaccaccttt tttggaagac gctgtgtccc tcaccggatc
      601 agcaggattc tggaaacaga tcgggaaatt cgatagcaat accgcagcat tgaaaatgat
      661 ttatcagatg atcccattcg tcgcggcaag ttcttaaaaa ctgtttgatt tccatacgaa
      721 gatatggact gaattcttct tctcttcaag gttcctgacc tggccagttg tcaattgaaa
      781 actgctcaaa tattcaacct atagctggtg ctgcggccac ctcccggatt ctggatcagc
      841 attcccttgt ccaccaaatc gacaagatcc ctgaacgccg tggccgtgcc tgctccagtc
      901 aggtgcgcgg ccttcctggt cgtcatcccg cccttgaaac cgccggatcc ggcgtccagc
      961 aaccggttaa gaatcttcgc ctggcggacg ttaatcgtct ttccaccgtg atcctgccag
     1021 aagcgggcct tctgcaacgc cacggcgacc tgttcgccag cttcatcgac agcgcttgtg
     1081 agacacccca gaaaccacac aatccaggca gtcagatcgg cgtcatacgc tgaagcccgg
     1141 cgcagctcat cgtaatatcg tggacgatcc ttcatgattg cagccgacat gctgaagtgc
     1201 cgccccggat atcctgatcc acgggcaagc gccatatccg cgatggctcg agccagccgg
     1261 ccgttgccat cctcgaacgg atggatggtc acgaaccgga aatgcgccag ggcggcgacc
     1321 acgaaagcgt ccatttcccc tggcggcgtc tcgaaccacc gcaggaataa atcaatctcc
     1381 gaatcaaggg agtctcccgg cggcgcaaca aaatgaacgg tttccctgcc cagcggtccg
     1441 gatacgacct gcatcgtcat gtcggctgga cggaaagaac ctggtgttat ccggcgcatc
     1501 cccgaccacc cggtcggaaa aagcgatgca tgccagccga gaatcctttc acgcgtcaac
     1561 ggcagggccg cgttcatggt tgcgtcaaca aggacgtcta caagcccctc ggcctgcccg
     1621 ggtgccggtg ttacgtccat cgaatcgacc tcgaggccca gccgccgggc gacgcttgac
     1681 ctgaccgagt cgagatcaag tcgttcaccc tcgatctcgg ctgtcctgac gacctcgatg
     1741 gctgttccgc gaacctgatc tgccagtctg gcacaaaagc caaggcccgt cagggatgca
     1801 acaagaacct caagccggga acgcgccctg tcgagcggtg gcccgactcg ctcgacgtcc
     1861 cacggaacat cgggccacct ttcggattgc catacccatt ccttcttcat ccgagtcccc
     1921 gcaatatgaa aggagggctg aacagactct gatgcgaata gaccagctaa tcacatcaaa
     1981 tttcgatttg attatcacat caagcccgtc ggcagatcaa tagtcaacca ctctgaccga
     2041 tcccctcatg gagagacaag tgtatttagt ggccgaggcg agactggagt aagtcacaat
     2101 tgacacaatg gactaagttg aggttagtat gcatgtccgg cctccgagtt tgaaagtgaa
     2161 gtcgaacatc gacaatcacg gggcgtacgc cgatggccgg ggcaggcggc ggggaagcct
     2221 ggagcggcat accccacccg atgcgatgcg agggcgtgaa caggattccg gatta
//
LOCUS       DAOPPA010000497         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_51816_length_2263_cov_3.294837, whole genome shotgun sequence.
ACCESSION   DAOPPA010000497 DAOPPA010000000
VERSION     DAOPPA010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51816_length_2263_cov_3.294837"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKH54_12465"
     CDS             complement(<1..499)
                     /locus_tag="PKH54_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00752.1"
                     /translation="MQNVYNQQFFDFDEGGIVAGKQKRKVDTQQNVPSYEEYQALYDA
                     AGDLRAAEPWKVIYSGDVIGVRDPETGIIGYCCVTGLLDEYLAMHVYLGGSGIEAYKA
                     FVDASDAYDGEPSPMDTMLAGRSITDIEVEFCNALELRPQDKARARDLGRSFVGRKAW
                     PAFMRN"
     gene            complement(627..1808)
                     /locus_tag="PKH54_12470"
     CDS             complement(627..1808)
                     /locus_tag="PKH54_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOD00753.1"
                     /translation="MNKQLKGSSAVVKPADAAVVSPDEELFGRVATILDHARVGVVRA
                     VNHHMVVAYWLIGREIVMELQRGQERAGYGERLVQNLSVKLTLSHGAGYSVENLWLFK
                     KFYSAYESRGSILYTACTELQPIETMKEAVDCAASAFRSELSWSHYRILMRVQSIIAR
                     DYYEAEAASSGWTVRELERQIHTQYYQRHVATSRRQANAKPAEGPLSTPGPKPVRNSD
                     ASLEKSNPIDALKDPYVMEFLDLPDPAALHEKGLEGAIIGHLRDFLLELGRGFAFVAR
                     QKRLSFDGDEMYVDLVFYNCILKCYLLIDLKMGRLTAADVGQMDSYVRMFDDLVAGDD
                     DNPTMGLILCTERNNAVARYSVLNDRRQIFASRYMTCLPTEAELAAEVERERRLFESP
                     R"
     gene            1915..>2263
                     /locus_tag="PKH54_12475"
     CDS             1915..>2263
                     /locus_tag="PKH54_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00754.1"
                     /translation="MDSSTNAGPATVRSIIEKGLSSLREETVPRIDRPLGDVMVTMTP
                     IQHFNILRANETDGSSYISSDEMDNISSEPQFVGGDSGAEYSGATCGLAALAGTAIPG
                     GGNCAVGDFVTLIS"
BASE COUNT          471 a          682 c          602 g          508 t
ORIGIN      
        1 cgttccgcat gaatgcaggc catgcctttc taccgacaaa ggatcgcccc aaatccctgg
       61 cccgggcctt gtcctgcggc ctgagttcaa gcgcgttgca gaactcaacc tcgatatcgg
      121 tgattgatct gccagccagc atcgtatcca tcggactcgg ttcgccatca taagcgtcac
      181 tggcatcaac gaatgccttg tacgcctcga ttccggaacc gcccagatag acgtgcatgg
      241 caagatattc atccaagagg cccgtcacgc aacaatagcc gatgattccc gtttcgggat
      301 cgcgaacgcc gatgacgtcc cccgaataaa tgaccttcca cggttccgcc gcccgaagat
      361 cgcccgctgc atcatacagc gcctggtact cctcgtagga tggaacattc tgctgcgtat
      421 ccactttgcg cttttgcttg ccggccacaa ttccaccttc gtcaaagtcg aagaactgct
      481 gattgtacac gttctgcatg aagacaactt ccgactttca aactctttgc tggccacatg
      541 gagatatggc ttctatctgt gcttctgttt gcattctgcc ggcactttca aataccgtag
      601 cctgaagcga tccgatcccg ccaaattcac cgggggcttt cgaacaagcg gcgttcgcgt
      661 tcgacttcgg cggccagttc ggcctcggtc ggcaggcagg tcatatagcg gctggcgaat
      721 atctgacggc ggtcgttcaa gaccgagtac cgcgcaaccg cgttgttgcg ttcggtgcaa
      781 agaatcaggc ccatggtcgg gttgtcatca tccccggcca ccaggtcatc gaacattcgg
      841 acatagctat ccatctggcc aacgtcggcc gccgtcagcc gccccatctt caggtcgatc
      901 agcagatagc acttcaggat gcagttataa aacaccaggt caacgtacat ctcgtcgcca
      961 tcgaacgaaa gccgcttctg ccgcgccacg aatgcgaagc cgcggcccag ttccagcagg
     1021 aagtcccgaa ggtggccgat aatggccccc tcgaggccct tttcgtgcaa cgctgcggga
     1081 tccggaaggt ccaggaattc catgacatag ggatccttca gcgcatcgat cgggttggac
     1141 ttttcaagcg atgcgtccga attccgaact ggcttcgggc cgggagtgga gagcgggcct
     1201 tcagcaggct tcgcattcgc ctggcgccgc gacgtcgcca catgccgctg gtagtactgc
     1261 gtatgaatct ggcgttcgag ttcgcgtacc gtccatccgc tggatgctgc ctcggcctcg
     1321 tagtagtctc gggcaataat actttgaacc ctcatcagga ttcgataatg agaccaggac
     1381 aactccgacc gaaatgctga cgccgcacaa tcgaccgctt ccttcattgt ttcaattggt
     1441 tgtaattctg tacacgccgt gtacagaatt gaccctctgg attcgtaggc tgaatagaac
     1501 ttcttgaaca gccaaagatt ctcaaccgaa tatccggccc cgtggctcaa tgtcagtttg
     1561 accgacaagt tctgaaccag gcgctcccca taccctgcac gctcctggcc gcgctgcagt
     1621 tccatcacga tctcgcggcc gataagccag tacgcgacga ccatatggtg attgaccgca
     1681 cgcacaacac ccacgcgggc gtggtcgagt atggtcgcaa cgcgtccgaa cagttcttca
     1741 tccggggaaa cgaccgccgc gtcggcgggt ttcaccactg ccgaactgcc tttcaactgc
     1801 ttgttcatga ctacaccgcc tgtcttgctg gattgtcctg gcgtacggcg gtgtctatcg
     1861 aatggtgacc acggcccgtt tcagtcggcg ggctcttttt caatcctggc gaaattggac
     1921 agttccacca atgccgggcc ggcgactgtc cggagcatca tcgagaaggg tttgtcgtcg
     1981 ctcagggaag aaacggttcc gcgcattgat cgcccgctgg gggatgtgat ggtgaccatg
     2041 acaccgatcc agcatttcaa tattctccgg gcgaacgaga ccgatggttc gtcgtacatc
     2101 tcaagcgatg aaatggacaa tatttcgtcc gagccgcaat ttgtcggcgg ggactcgggt
     2161 gccgaatatt cgggtgcaac ctgcggactc gcggctcttg ctggaacggc aatacccggc
     2221 ggtggcaact gtgcggtggg ggactttgtc actctaatct cat
//
LOCUS       DAOPPA010000498         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_51988_length_2257_cov_3.054042, whole genome shotgun sequence.
ACCESSION   DAOPPA010000498 DAOPPA010000000
VERSION     DAOPPA010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51988_length_2257_cov_3.054042"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="gcvPB"
                     /locus_tag="PKH54_12480"
     CDS             <1..1272
                     /gene="gcvPB"
                     /locus_tag="PKH54_12480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOD00755.1"
                     /translation="VDTGFYPLGSCTMKHNPRICEDTAALEGFAGIHPMTPSSMAQGA
                     LALMWELEQSLKEILGFSAVTLLPAAGAHGELTGLKVMRAALTARGNPRTKVLIPDTA
                     HGTNPATSALTGYTVVKVKSNESGILDPAEVAAVMDGDTAGIMITNPNTLGLFERNIH
                     QVAAIVHEKGGLVYGDGANLNAIMGKYRPACLGIDVMQLNLHKTFSTPHGGGGPGSGP
                     VGVVESLVDFLPVPRVVRVEDGKFELREDFPLSIGRMRSFQGQFGVLVKALTYIRALG
                     ADGLARAAEMAVLNANYVRVKLQDKFNLPYPGVCMHEAVFSDRNQIKSEGKVSTMDMA
                     KRLMDHGIHPMTVYFPLIVSGAMMIEPTETENMADLDEFIAVMERIADEAANNPEIVH
                     SAPHLTSVRRPDEVSAARNPVLTWHAGNRQA"
     gene            1358..>2257
                     /locus_tag="PKH54_12485"
     CDS             1358..>2257
                     /locus_tag="PKH54_12485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD00756.1"
                     /translation="MATRLAAIVAFLLLFYQAGSGSLLPSDDSAYARAAIEGTRGGRL
                     ADVTWMGHPLFEKGPVLFSAIQIGGLAGGSVELQARLPGIVFGLMVLLLIFRIGLDLG
                     LSRQASILAAALCLATSVFFFNARRPMTDIPGLMFALAGFRAMTIARWRSQAFLGGVM
                     FGLSSLCKLTSPVPFIIAAVAARIAGYRPRADSEPDDTLSPAAWSVLGLAGGLAAFLP
                     WHAWMAVEHGSRFIETYFGYHLFGRMSAAVVGTGRAEAYLGWLASRDVVGAALLAITF
                     PVAMLLAIRRNRPAFVVLLLVLLP"
BASE COUNT          401 a          713 c          705 g          438 t
ORIGIN      
        1 gtcgataccg gattctatcc tctcggttcc tgcacgatga agcacaaccc caggatatgc
       61 gaggacaccg ccgccctgga aggatttgcc ggcatccacc cgatgacgcc ctcatcgatg
      121 gcacagggcg ccctggcact gatgtgggag cttgaacagt ccctgaagga aatcctcggg
      181 ttctccgcgg tgacgcttct tccggcggcg ggagcccacg gcgagttgac cggcctgaag
      241 gtcatgaggg cggccctgac cgccagggga aaccccagga cgaaggtcct tatcccggac
      301 acggcccacg ggaccaaccc cgcgaccagc gccctgaccg gctacacggt cgtgaaggtc
      361 aagagcaacg agtccggcat ccttgaccct gccgaggtcg cggccgtcat ggacggcgac
      421 accgccggca tcatgatcac gaacccgaac acgctcgggc tttttgaacg caacatccac
      481 caggttgccg ccatcgtcca tgaaaaaggc ggcctggtct atggcgacgg cgcgaacctt
      541 aacgcaatca tgggaaagta tcgcccggcg tgccttggaa tcgacgtcat gcagttgaac
      601 ctgcacaaga ccttctcgac gccgcacggc gggggcggcc cgggaagcgg tcccgtcggc
      661 gtggtcgagt cgctggtcga tttcctgccg gttccgaggg tggttcgtgt cgaagacggc
      721 aaattcgagc tgcgggagga cttcccgtta tcgatcggcc gcatgcggtc gttccagggc
      781 cagttcggag tcctggtgaa ggccctgacg tacatcaggg cccttggggc cgatggactg
      841 gccagggccg ccgagatggc cgtcctgaac gccaactacg tccgcgtgaa gctgcaggac
      901 aagttcaacc tgccctatcc gggcgtctgc atgcacgagg cggtcttctc ggatcgcaac
      961 cagatcaagt ccgagggcaa ggtttcgacc atggacatgg ccaagcgcct gatggatcac
     1021 ggcattcacc cgatgaccgt gtatttcccg ctgattgtaa gcggtgccat gatgatcgag
     1081 ccgaccgaaa cggagaacat ggcggatctg gacgagttca tcgccgtgat ggaaaggatt
     1141 gccgacgagg ccgccaacaa ccccgagatc gttcattcgg caccccacct gacatcggtc
     1201 aggcggccgg acgaagtctc cgcggcacgc aacccggtgc tgacctggca tgccggcaac
     1261 cggcaggcct gacgaacgga cttccgacac ctttcatttg cgttacaatc cgccaatgac
     1321 aaccagcagc gcaaccattg caaacactat ctccctaatg gccaccaggc tggcagcaat
     1381 cgttgcattc ctcctgctct tctatcaggc cggttccgga agcctgcttc cttcggacga
     1441 ctcggcgtat gcgcgtgccg ccatcgaggg aactcgcggc gggaggcttg ccgacgtgac
     1501 gtggatgggg cacccgctgt tcgaaaaggg gccggtgctg ttttcggcga tacagatcgg
     1561 cggacttgcc gggggatccg tcgaactcca ggcccgcctt cccggtatcg tgttcggcct
     1621 gatggttctt ctgctcatat tccgcatcgg ccttgatctg ggactttccc ggcaggcgtc
     1681 cattctggcc gccgcactgt gtctggccac cagcgtgttc ttcttcaacg cccggcgccc
     1741 gatgaccgac attcccgggt tgatgttcgc acttgccggt ttcagggcca tgacgattgc
     1801 aaggtggcga tcacaggcat tccttggcgg ggtgatgttc gggttgtcat ccttgtgcaa
     1861 gctgaccagc ccagtcccct tcatcattgc ggccgtggcg gcgaggattg caggataccg
     1921 accgcgagcc gattcggagc cggacgacac gctgtcgcct gccgcatggt ccgtgctcgg
     1981 ccttgcgggc gggctcgcgg cattccttcc gtggcatgcg tggatggccg tggaacacgg
     2041 ctcgcggttc attgaaacgt acttcggata tcacctgttc ggacgcatgt cggcggcggt
     2101 ggttgggacg ggaagggcgg aggcatacct gggctggctt gcttcccgcg acgtggtcgg
     2161 cgccgccctg cttgcaatca cctttcccgt cgccatgcta ctggcaatcc gccggaaccg
     2221 gccggcattt gttgtgctcc tgctggtgct gctgccg
//
LOCUS       DAOPPA010000499         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_52581_length_2238_cov_3.841961, whole genome shotgun sequence.
ACCESSION   DAOPPA010000499 DAOPPA010000000
VERSION     DAOPPA010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52581_length_2238_cov_3.841961"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKH54_12490"
     CDS             complement(<1..549)
                     /locus_tag="PKH54_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00757.1"
                     /translation="MRNVMFGLCVLFVAAAVGCKKEAPAPAPVAAAPEHEKMAAPDVN
                     KDEIQRVASNFVDDYGTVVGGLYKESALAYWKAANTGDKASFDEYAAKELEVRKYLSD
                     TDRYAKLQQLRKHGNLLEPVTARALEVIELAYKENQLPADMLKKLVEKSSEIEMTFKT
                     HRAEIDGKKYSNNELLDVLGAET"
     gene            complement(649..2172)
                     /locus_tag="PKH54_12495"
     CDS             complement(649..2172)
                     /locus_tag="PKH54_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD00758.1"
                     /translation="MARRISLFAKILFWFFVNLLIGGGIVWYAFDLDFRVGPDSVLYS
                     RSPNRIPILGLSLMKDLSDFQVDRWSEELERYSGIYGVDVSIFDVGGEWVAGPRVSLP
                     VALRARMVEQFGLRTDENHPSSEAQPGDHPGHDLFCRISNGHCPFCSSGGYGLPDQRH
                     PSFKMKTSQPNFYWAVMRLPGLRVSPGSQRELVLVGRSSSVTGNGLFIDPTPWAILAV
                     LGMIISTLLWIPMVRHLTVPIRQMTEVTEKIARGVFDSRVGSRRTDELGRLGRAINNM
                     ADRLALMIYGQKRFLGDAAHELASPLARMQLAIGLLEESVDEKGRQRVAQLKDDVEQM
                     TELVNGLLSFSRSDSVSGRVTIDCVNLFDVIARVIDREVPDESIAISFDVPERIDVMA
                     DRDLVSRIFANLVRNAVRYAGHAGPIAISASEKGQFVYVTVADCGPGVPEKDIPQLFE
                     PFFRVEQSRVREAGGVGLGLAIVKTAVIACGGTVSATNRKDGGFEVTVALPACTVQA"
BASE COUNT          495 a          666 c          613 g          464 t
ORIGIN      
        1 cgtttcggcg cccaggacgt ccagaagctc gttgttcgaa tatttctttc catcgatttc
       61 ggcacggtgg gtcttgaagg tcatttcgat ttccgacgac ttctcgacca gtttcttcag
      121 catgtcggcc gggagctgat tttccttgta cgcaagctcg atgacctcaa gggcgcgggc
      181 cgttaccggt tccagaaggt tcccgtgctt gcgaagctgc tgcaacttgg cgtaccgatc
      241 ggtatccgac aggtacttgc ggacttccag ttcctttgcc gcgtattcgt cgaaggaagc
      301 cttgtcgccg gtgttggcgg ccttccagta ggcaagggcg gattccttgt acaatccgcc
      361 aaccaccgtg ccgtagtcat caacgaagtt ggacgcgaca cgctggattt catccttgtt
      421 gacatcgggt gctgccatct tttcgtgctc cggggccgcg gcgaccggtg ccggcgcggg
      481 tgcttccttt ttgcagccga cggcggccgc gacaaacaga acacacaatc caaacattac
      541 gttccgcatc ctttcctcca gacagggcgt tgacggatca tcatccggcg aagcgatatc
      601 gtcaaccggc aatgtgtcac tggggacggc cgctggacaa agaagttctc aggcctgaac
      661 ggtgcaggcg ggcagggcga ctgtaacctc gaatccaccg tccttgcggt tggtggcact
      721 taccgttccg ccgcaggcaa taaccgcggt cttgacgatc gcaaggccaa ggcccacacc
      781 gcccgcttcc ctgactcgtg actgctcgac acggaagaat ggctcaaaaa gctgtgggat
      841 gtccttttcg gggactcccg gcccgcagtc ggcaactgtc acgtacacga attggccctt
      901 ctccgatgcg gaaatcgcga tcggcccggc atggcctgcg tacctgaccg cgttgcggac
      961 aagattcgcg aaaatccttg aaacaaggtc gcgatcggcc attacatcaa ttcgttccgg
     1021 gacatcgaag gaaatcgcga tcgactcgtc gggaacctcg cgatcgatca ctctggcaat
     1081 gacatcgaag agattgacgc aatcgatggt cacccgccca ctcaccgaat ccgagcggga
     1141 aaacgacaga agcccgttaa ccagttccgt catctgttcg acgtcatcct tcaattgggc
     1201 aacgcgttgt cgcccctttt catcgaccga ctcctccaga agtccgatgg caagctgcat
     1261 tcttgcaagg ggggatgcaa gctcgtgcgc ggcatcccca aggaaccgct tctgaccgta
     1321 aatcatcaat gcaagcctgt cggccatgtt gttgattgcc cgaccgaggc ggcccaactc
     1381 gtccgtgcgc cgggacccga ccctgctgtc aaagacgccg cgtgcaatct tttccgtgac
     1441 ttcggtcatc tgccggattg gaactgtcag atgacgaacc atcggtatcc agagaagcgt
     1501 cgaaatgatc attccaagaa ccgcaagtat tgcccacggg gttggatcga tgaaaaggcc
     1561 gttgccggtg accgaacttg atcgccccac cagtacaagc tcgcgctggc ttccgggtga
     1621 aacgcgaaga ccgggcagcc gcatcacggc ccagtagaaa ttgggctggg atgtcttcat
     1681 cttgaagctt ggatgcctct gatcaggcag gccgtatccc cccgatgaac agaaaggaca
     1741 gtgaccattg gaaatccggc agaacagatc gtggccggga tgatcgcccg gctgcgcttc
     1801 agaactgggg tggttttcat ccgtgcgaag gccgaactgc tcgaccatcc ttgcgcgaag
     1861 cgcaacggga agggaaactc ggggccccgc gacccactcg ccgcccacat cgaagatcga
     1921 gacgtcaaca ccataaatgc ctgaatacct ttcaagctct tccgaccacc ggtcaacctg
     1981 gaagtccgaa agatccttca tcaggctcag ccccaggatt ggaatgcggt tcggacttct
     2041 ggagtacagg accgagtccg ggccaacccg gaagtccagg tcgaacgcgt accagacaat
     2101 gccaccgccg atcagcagat tgacgaaaaa ccagaaaaga atttttgcga aaagactgat
     2161 ccgtcgggcc atccattact cctgctcagg atcaatgaac atatagccga tggatcttac
     2221 agttttaatg aacttcgg
//
LOCUS       DAOPPA010000500         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53021_length_2225_cov_3.823041, whole genome shotgun sequence.
ACCESSION   DAOPPA010000500 DAOPPA010000000
VERSION     DAOPPA010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53021_length_2225_cov_3.823041"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1327
                     /locus_tag="PKH54_12500"
     CDS             173..1327
                     /locus_tag="PKH54_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD00759.1"
                     /translation="MKFVFFAVLIVAWLAAHRYVFVRLRESLRLAPAIRFTIGSLMIL
                     MAVSWPVSRMLSRIGENLAVHLFQGAGSYWMGFMAVLVMTIFATDLLIALPARIGNRR
                     LVLSQGALVAARKVSFLVAVASAVVVSTIAMAVATGPVAVRKVDIASPAVPAALDGRT
                     LLFLSDIHHGSLVGVDDLDRIEAAVKSVQFDVILFVGDLTDEENGGDGSAFERFASWG
                     RLGSSGRAFAVTGNHERYSGGDAVVGRLQQAGIQVLRQQAECNDAGLCLAGVDDPAML
                     PDGVGFDAAVVRAVSGVPDDAFLVLLSHQPVMVDFVAGTGVDLMVSGHTHHGQFPPAT
                     FFTPLFYPYWAGLYRIRDMFLYVSSGAGFWGPPLRLGSHPEVVALKLRRI"
     gene            complement(1517..>2225)
                     /locus_tag="PKH54_12505"
     CDS             complement(1517..>2225)
                     /locus_tag="PKH54_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00760.1"
                     /translation="DLPALVKAATSLGLAVGLQTNGRMLAYPEVREGLQGLSIRFVIA
                     LHGPDAFVHDAVTRAPGSFEQTLEGIRALAAAGEKITLKILMSRINADRLEDLARIGF
                     AAGAREFNFTFPHGLGNAATEHAVAIPSFMEIMPSLTAAMEFLDASGCTYVTESVPLC
                     HLGSRMNHASELIFAAQFKSEVRQLDQAARDWSRDRVQGKTHPPECAGCRMLSRCEGI
                     WTEYFDFNRDAGVTPIR"
BASE COUNT          342 a          653 c          708 g          522 t
ORIGIN      
        1 ggtacttcgc gggcagctgc gtgggagagt aggtcgttgc cggccccttt ttttttcctt
       61 ttcatttcat caaatcatca agttgtattt cttgaacggc ggtgtggcgt gctaaccttg
      121 ccgactggcc gtcgcctttt gcgccgcgcc gcatgcggag ggattcatcc cgatgaagtt
      181 cgtgttcttt gccgtgctta tcgtggcgtg gcttgccgcg caccgctatg ttttcgtgcg
      241 tctgcgcgag agccttcgcc tggcgcccgc gatccgcttc acgattggtt cgcttatgat
      301 cctgatggcg gtatcctggc cagtctccag gatgctgtcc aggatcggcg agaacctggc
      361 cgtccatttg ttccaggggg ccggttccta ctggatgggc ttcatggccg tcctggtcat
      421 gacgatcttt gcgacggacc tgttgattgc cctgcccgcc cgtatcggga atcgcaggct
      481 tgtcctgtcg cagggcgccc tggtcgcagc gcggaaggtg tcattcctgg ttgcggtcgc
      541 ctcggcagtc gttgtttcga cgatcgcgat ggcggtggcc accggcccgg tcgcggtcag
      601 gaaggttgac atagcttccc cggccgtccc ggcggcgctt gacggacgta ccctgctctt
      661 cctgtccgac attcaccatg gcagcctggt cggcgttgac gatctggaca ggattgaggc
      721 cgcggtcaag tcggttcagt ttgacgtgat cctgttcgtg ggtgatctga cggacgagga
      781 aaacggtggt gacggttcgg cgttcgaaag gtttgcctcc tggggccggc tcggaagttc
      841 cggcagggcc ttcgccgtga ccggcaatca cgagcggtac tccggcggcg acgccgttgt
      901 cgggcgtctg cagcaggccg gcatccaggt gcttcgccag caggcggaat gcaacgacgc
      961 cgggctttgt ctcgcgggtg tcgatgaccc ggcgatgctg ccagatggag ttgggttcga
     1021 tgcggcagtc gtccgggcgg tttcgggcgt tccggatgac gcctttctcg tcctgctttc
     1081 gcaccagccg gtgatggtcg atttcgtggc cggaaccggt gtcgatttga tggtttccgg
     1141 ccacacgcat catggccagt tccctcccgc gaccttcttt accccgcttt tttatcctta
     1201 ctgggcaggg ctttaccgga tacgcgacat gttcctgtac gtttcgtccg gcgccggttt
     1261 ctggggcccg cccctgaggc tcgggagcca tcctgaggtc gttgcgctga aactgcggcg
     1321 aatttgaagg cgggccggcg gaatggcgtt acggcgggcg aattttcatt cgcccgtaca
     1381 gtggctggat ttcattcgcc cgtacagtgg ctggatgtac gctgcgttcc ttgtacgggc
     1441 ggattacatc cgcccgctgc ggttcagcac gttgccatca ggccccggtt ttcgcgccga
     1501 tcagccgatc agccgatcaa cggatcggcg tcaccccggc atcccggttg aagtcgaagt
     1561 actccgtcca gatcccttcg catctggaaa gcatcctgca tccggcgcat tcgggcgggt
     1621 gtgtcttgcc ttgaacgcga tcccggctcc agtccctggc ggcctggtcg agctggcgaa
     1681 cctcggattt gaactgtgcg gcgaagatca gttccgacgc gtggttcatc cggctgccaa
     1741 gatggcacag tggaaccgat tcggtgacgt acgtgcatcc gcttgcatcg agaaattcca
     1801 tggcagcggt cagggacggc atgatttcca tgaatgaagg tatcgcaacg gcatgttcag
     1861 tggcggcgtt tccaaggcca tggggaaagg tgaagttgaa ttcgcgcgcc ccggccgcga
     1921 acccgatcct ggcaaggtct tcaagccggt cggcgtttat gcgcgacatc aggatcttca
     1981 gagtgatctt ttcaccggct gccgcaagcg cccggattcc ttccagggtc tgttcaaacg
     2041 aaccgggcgc cctggtaacg gcgtcgtgaa cgaaggcatc cggcccgtga agcgcgatta
     2101 cgaaacggat tgaaagtccc tgaagtcctt ccctgacttc ggggtacgca agcatccggc
     2161 cgtttgtctg aagtccaacc gcaagtccaa gcgatgtggc ggcctttacc agtgcaggca
     2221 gatcc
//
LOCUS       DAOPPA010000501         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53077_length_2223_cov_3.159594, whole genome shotgun sequence.
ACCESSION   DAOPPA010000501 DAOPPA010000000
VERSION     DAOPPA010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53077_length_2223_cov_3.159594"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1825)
                     /locus_tag="PKH54_12510"
     CDS             complement(92..1825)
                     /locus_tag="PKH54_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00761.1"
                     /translation="MRHLGILLALPLLLVACGGSDPGQDVMPDGIGDVTYDAVGDTVV
                     ADQGTDVAVPDQGTDESDPDMTEEVQAEVVEDVLQDAGSDTVADATPNFDEIVQPYPP
                     RELPFTFTRPAEGEPLSAQEVTDFTKKVAGAYKDVGFFRWLLRTSTGVDATSGREGYL
                     SWYNDVVAVKSGDTVTFSHRGGEHNMWIPGSIVLASVMNAYLLTGDWEMARLTEQYCK
                     GLTAVVKGFVFDENDTETALMARAILPFDHSFTLDADFWKDDGRKKVVEFHEAQKIED
                     GWNAHTFNWPTNPTWGDIWVTNMRSKDDVRAISRTVTWLPYVVTDAADEWVRTACQET
                     LDTMVAFHADIVDNDYFIRTKGVDGVAYRIPCDDQDLGSYSCYIEMDERNECCARLSA
                     DMIAYGERRTNDCGTCTGSIYDQFASVAHYYNMPIIWDYHMAGVGTSLLKRQYRDAWH
                     ALRGLAERIDSYMHPTEDVPNTDNHNWDSDMAVLLVQAASMGLPLTDMEARHVQKHFS
                     AAADYFKSWTNWDLWALEDGQYGVRPPDNDQAVDSEAFAILFEYCNSPFKNPAGAAFV
                     DCAVLADPSSW"
     gene            1962..>2223
                     /locus_tag="PKH54_12515"
     CDS             1962..>2223
                     /locus_tag="PKH54_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOD00762.1"
                     /translation="MPRPQNCRRIGHVTREMRFKPAGVPCTEIDAVSMGFDELEAIRL
                     TDLEGMYQEAAAERMGVSRPTLSRIIESARRKVALALVNGLEL"
BASE COUNT          456 a          677 c          654 g          436 t
ORIGIN      
        1 ggcgaggccc acgaaaagcg cttctacatg gaatgccgcc gccttggatg atgccggccc
       61 gccgcaacca acgcagattt caatacgtgg actaccagga agaaggatcg gcaaggacgg
      121 cgcaatcgac gaaggcggcg ccggccggat tcttgaacgg gctgttgcag tattcgaaaa
      181 ggatcgcgaa cgcctcggaa tcgacggcct ggtcgttgtc gggcggacga acgccgtact
      241 gaccatcctc cagcgcccac agatcccagt tggtccacga cttgaagtag tcggcggccg
      301 cgctgaagtg cttctggacg tggcgcgctt ccatgtcggt cagcggcagg cccatcgagg
      361 cggcctggac cagaagcacg gccatgtcgc tgtcccagtt gtgattgtcg gtgttcggga
      421 cgtcctcggt cggatgcata taactgtcga ttcgttcggc aagcccgcgc agcgcgtgcc
      481 atgcgtcgcg atactgcctt ttcagcaggc tggtcccgac accggccatg tgataatccc
      541 agatgatcgg catattgtag taatgggcaa ccgacgcgaa ctggtcgtaa atactgccgg
      601 tacacgttcc gcagtcattc gtcctgcgct cgccgtatgc aatcatgtcg gccgaaaggc
      661 gcgcgcagca ttcgttgcgt tcgtccatct cgatgtaaca ggaatagctg ccaagatcct
      721 gatcgtcaca tggaatccgg taagcgacgc catcgacgcc cttggttcgg atgaagtaat
      781 cgttatcaac gatatccgcg tggaatgcga ccatcgtgtc cagggtttcc tggcacgcgg
      841 tcctgaccca ctcgtcggcg gcgtccgtca cgacataagg caaccacgtc acggtgcggg
      901 aaatggccct gacgtcgtcc ttgctgcgca tgttggtgac ccagatgtcg ccccacgtcg
      961 gattcgtcgg ccagttgaag gtgtgagcgt tccacccatc ctcgatcttc tgggcctcgt
     1021 ggaactcgac taccttcttg cggccatcgt ccttccagaa atcggcatca agcgtgaacg
     1081 aatggtcaaa cggcaggatg gcgcgggcca tcagggcggt ttccgtgtcg ttctcgtcga
     1141 acacgaatcc cttgaccacg gcggtaagcc ccttgcaata ctgctcggtg agcctggcca
     1201 tttcccagtc gccagtcagc aggtacgcgt tcatgactga tgccagcacg atcgagccgg
     1261 gaatccacat gttgtgctcg ccgccgcgat ggctgaaagt caccgtgtcg ccggacttta
     1321 ccgcgacaac gtcgttgtac caggacaggt acccttccct gccggaagtc gcatcgaccc
     1381 cggtacttgt gcgcagcagc cacctgaaga acccgacatc cttgtacgcg ccggcaactt
     1441 tcttcgtgaa atcggtcact tcctgggccg agaggggctc gccttccgca ggacgcgtga
     1501 acgtgaaagg cagttcgcgt ggcggatatg gctggacaat ctcgtcaaaa ttcggggtcg
     1561 cgtccgcgac ggtgtcggaa ccggcgtcct gcaggacgtc ctcgacaacc tcggcctgga
     1621 cttcttccgt catgtcggga tcgctttcat cggtcccctg atcgggaacc gcgacgtctg
     1681 ttccctgatc cgcaaccacc gtgtcgccca cggcgtcgta ggtaacgtcc ccaatcccgt
     1741 cgggcatgac gtcctgcccc ggatcggaac cgccacaggc cacaagaagt aacggaagag
     1801 caagaagaat tcccaaatga cgcatcgaca gacctccttc ggcgggaccg ccccgccggg
     1861 cgattttata cgttttggaa gaacaagacc atcatgggaa ccacaacgtc cgcggcttga
     1921 caattttgaa catttgttca taatcccgca ggaggtgacg aatgccaaga ccacaaaact
     1981 gccgcaggat cggccacgta acgcgggaaa tgcggttcaa gccggccggc gttccgtgta
     2041 cggaaatcga cgcggtatcc atgggttttg acgaactcga ggccatcagg ctgacggatc
     2101 tcgaagggat gtaccaggag gcggccgcgg aacggatggg ggtatccagg ccgacactca
     2161 gccgcatcat cgaatccgcc cgaaggaagg tcgcgctcgc cctggtgaac ggtcttgaac
     2221 tcc
//
LOCUS       DAOPPA010000502         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53207_length_2219_cov_3.957486, whole genome shotgun sequence.
ACCESSION   DAOPPA010000502 DAOPPA010000000
VERSION     DAOPPA010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53207_length_2219_cov_3.957486"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PKH54_12520"
     CDS             complement(<1..553)
                     /locus_tag="PKH54_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD00763.1"
                     /translation="MKCEDASRYYDLYLDGELDSSEMRDVELHLGSCSRCAMAVGREK
                     LFRQRFSERMASLKAPASLDAVIRARTARRRAVLEPRNLAIAASILVVLGVGIPVFVS
                     VTSPVGDIDDASSSVVRAHRQSSDSEVYGSRQAVQGFVRARAPFPTEIPVDDAEGRRL
                     IGARLTSIQDRPAVIYLYDVGGRR"
     gene            complement(550..1206)
                     /locus_tag="PKH54_12525"
     CDS             complement(550..1206)
                     /locus_tag="PKH54_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD00764.1"
                     /translation="MKILPFPKSRKERFEKEALPLLDTLYRFALSRTGDSSEADDLVQ
                     ETFMKAWDAFDSYESGTNCRAWMCRILRNTHINRIRASSKEFVTDEIPESTAGQSLPG
                     WAFFDFEAPDDASWLKFTRQQIMGAFERMPQEFRSVVELADVEGFSYREIADALGIPI
                     GTVMSRLHRGRRLMREMLLAVGVGPSSGATASREAAADDGDEGVLSLDGYRRTGGGVS
                     "
     gene            complement(1317..1976)
                     /locus_tag="PKH54_12530"
     CDS             complement(1317..1976)
                     /locus_tag="PKH54_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00765.1"
                     /translation="MIQQSAAKVELRAFSFIDSLQPQLASFIATVSRGFLPMEGDAAL
                     FVEISPGLEINVLADLVFKGTTCRPGMLVVERAYGLLQVHSPDQGQVRQAGRIVLEHL
                     GLSEAEALKPKIISSQIITGVDNHQSHLINRLRHGQFLLAGDAFYILEIHPAAYAAIA
                     ANEAEKVAPIQNLEVMTFGAFGRLYLGGPDDNIKEAAAAVERIVNALPGRENQVKSLL
                     Y"
BASE COUNT          485 a          701 c          614 g          419 t
ORIGIN      
        1 ctcgccgacc gccgacgtcg taaagataga ttaccgcggg cctgtcctgg atactggtca
       61 gtcgagcccc tatcagacgg cgcccctcgg cgtcatcgac cggaatctcg gtcggaaatg
      121 gagccctggc cctgacgaag ccctgaacgg cctgccggct gccgtaaacc tcggaatcgg
      181 aactctggcg atgcgcgcga acaacgcttg aggatgcgtc gtcaatgtcc ccgaccggac
      241 tggtaacgga aacaaaaacg ggaataccga caccgagcac cacaagtatc gacgccgcaa
      301 tcgcaaggtt gcgcggctcc agcacggccc gccggcgggc ggttctggcc cttatcacgg
      361 catcgagact ggccggcgcc ttcagagacg ccatacgctc cgaaaaccgc tgcctgaaaa
      421 gcttctcccg gccaaccgcc atcgcgcacc gcgagcagga accgagatga agctcaacat
      481 ccctcatctc ggacgagtca agctccccgt caagataaag gtcatagtac ctcgatgcat
      541 cttcacactt catgatacgc ccccgccggt cctcctgtaa ccgtcaagac tcaggacacc
      601 ttcgtcgcca tcgtcggcgg ccgcctcccg ggacgcggtg gcgccgcttg acggacctac
      661 gccaaccgcc aggagcatct cgcgcatcag gcgccgcccg cggtgcagcc gcgacatcac
      721 ggtcccaata gggattccaa gcgcatcggc gatttccctg tacgaaaacc cttcaacatc
      781 ggccagttca acgacggaac ggaattcctg gggcatccgc tcgaaagcgc ccatgatctg
      841 ctgcctcgtg aacttcagcc acgacgcgtc atccggcgcc tcgaaatcga aaaaggccca
      901 gccgggaagg gattgccctg cggtcgattc cgggatctcg tcggtcacga actccttcga
      961 cgacgccctg atccggttga tgtgggtatt gcgcagaatc ctgcacatcc atgcacggca
     1021 gttcgttccg ctctcgtagc tgtcgaatgc atcccaggcc ttcatgaaag tttcctgcac
     1081 gagatcgtcc gcctcggagg aatccccggt tcgcgacagc gcaaacctgt agagcgtatc
     1141 cagaagaggc agagcttcct tttcgaagcg ttccttcctt gacttcggga aaggcagaat
     1201 cttcatgatt cctccatgcg accacgccag gacaaacaac ggctggcacg gatgtattcc
     1261 cgttgggggc ccgcccgatg aaaacgccgg gccgaagggt accggggcaa gccggttcag
     1321 tacagcagcg acttcacttg gttctcccgt cccggcaggg cattcacgat cctttcgacg
     1381 gccgcggccg cttccttgat gttgtcgtcg ggaccaccaa ggtaaagacg accgaatgcg
     1441 ccgaaagtca tcacctccag gttctggatc ggggcgacct tctcagcctc gttcgcggca
     1501 attgccgcat aggcggccgg atgaatctcg aggatataga acgcatcgcc ggcaagcagg
     1561 aattgcccgt gacgcaagcg gttgatcaga tgggactgat ggttatccac gccagttatg
     1621 atctgggacg aaatgatctt cggcttcagt gcttccgcct ccgaaagccc aaggtgctca
     1681 agaacgatcc tgcctgcctg gcgaacctga ccctgatccg gcgaatgaac ctgcagcagc
     1741 ccgtacgcac gctccacgac cagcatcccg ggcctgcacg tggttccctt gaaaaccagg
     1801 tccgcaagca cgttgatttc cagtcccggc gagatttcga cgaacagcgc cgcgtcgcct
     1861 tccatcggaa gaaagccgcg actgaccgtg gctatgaagg aggcaagctg cggctgcagg
     1921 ctgtcgatga aagagaatgc cctcagttcc accttcgcgg cgctttgctg tatcaaggtg
     1981 tttccccgaa aatccggcag ccgcaaggct atcagaaaac acgtcagaag ttcaacgccg
     2041 ttgcggtgac gaaacaggcg tgcggatcat gcgataagac agttcgtgct ccgtcatcgc
     2101 cggtcgaacc atgccggccg atgcattgga ttaatatcta ttgagtattg aagttgtaaa
     2161 actgtattat tgcgatgtat tcggaggtct tgggccatga aaagccgact cttcaagtt
//
LOCUS       DAOPPA010000503         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53480_length_2210_cov_4.976798, whole genome shotgun sequence.
ACCESSION   DAOPPA010000503 DAOPPA010000000
VERSION     DAOPPA010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53480_length_2210_cov_4.976798"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKH54_12535"
     CDS             complement(<1..680)
                     /locus_tag="PKH54_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOD00766.1"
                     /translation="MALSDSLLDQVRNCGVVGSGGAGFPTHVKLAAQVDTLVINAAEC
                     EPVLVTDQWLTISAAADVVDGIRAVARQVGAGRIVIGIKEHYHAMIAALRPHLAGAPA
                     IEFHFFQDFYPAGDEHVMVHDITGRIVPEGGIPLDVGVVVQNVSTMHAVRRAMFDQPF
                     IRKAVTVAGEVARPGVYEAPIGMSAAEVINAAGGSTLDRFEVIDGGPMMGGLQKSIED
                     PVIKTTAG"
     gene            815..>2210
                     /locus_tag="PKH54_12540"
     CDS             815..>2210
                     /locus_tag="PKH54_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOD00767.1"
                     /translation="MKFARIFVVMMALAMPFFGNSGCSCDDKKVGDDLVFAYNPVSLD
                     FGSVSLNQSKTLKLTVFHSGTSGTVVVNGIALEGVSSDEFSYSVIEGAAEEWASYSSI
                     SLDVGESAVISVTYTPKNSGTDNGRIVFAHNVATQGNKTSVNITTSGQTGELTAEPSP
                     IDFGDINAGEEATLDVTITNNGTFTVDVTDVYLDYEGSFDFTVLDVTSKDGPTPVTAM
                     APGEVLNVTLQYKPVGTGYDGSNLIVLGNVEDNLRQWSFPVFGSEIGPRIQVIPQQIE
                     FDFVPIDEERERSITVSNIGNTNLSITSVGTSMGSSPDLEIVNPPEVGAPVVIKPEQN
                     YEFKVKWTAKTQSANPEAPIGAVTIVSNDLSNSPNVVPVYGQVEAPSIEVLPAQIDFG
                     FGAQNKAKSALLTIKNNGFGALTISKLEIINPSTTQYGQEFAITYNAGFPVTNSVNPA
                     PSDIPGNSFTPVELT"
BASE COUNT          504 a          693 c          581 g          432 t
ORIGIN      
        1 tacccggccg tagtcttgat gaccggatcc tcgatgcttt tttgcaatcc gcccatcatc
       61 gggccgccat cgatgacctc gaaccgatcc agcgtggatc cgccggccgc attgatgacc
      121 tcggcggcgg acattccaat cggcgcttca tagacgcccg gcctggcgac ctcgccggca
      181 accgtcaccg cctttcgaat gaacggctga tcgaacatcg cccgacgaac cgcatgcatt
      241 gtcgagacgt tctgaaccac gacgccgacg tcaagcggta tgccgccttc cggaacaatc
      301 ctgcccgtga tgtcgtgaac catcacatgt tcatcaccgg ccggataaaa atcctggaaa
      361 aaatggaact cgatcgcggg cgcgcccgcc aggtggggac gcaacgcggc aatcatcgcg
      421 tggtagtgtt ccttgattcc gatgacgatg cggccggccc cgacctgcct cgcgaccgcc
      481 ctgatgccgt caacgacatc ggcggcagcc gatatggtca accactgatc ggttaccagc
      541 accggctcgc attcggcggc attgatgacg agggtatcaa cctgcgccgc aagcttcacg
      601 tgggtcggga accccgctcc gccggagccg accaccccgc agttccttac ctgatccagc
      661 aacgagtctg aaagcgccat gtcgcctcca cgatttcgcg aggaatgata tcacatgaaa
      721 aaaacggttt tttcaatttt catttactgt agaatcatac ggcctgtttc ttaacaagcg
      781 ggtcaaggat ttcaatccgt tggagggtgc cctgatgaaa ttcgcacgca tctttgtggt
      841 tatgatggct cttgccatgc cgttcttcgg caactccggc tgttcctgcg acgacaagaa
      901 agtcggcgac gaccttgtgt tcgcatacaa cccggtttcg ctcgactttg gttcggtctc
      961 gctgaaccag tcaaaaacgc tgaagctgac ggtgttccat tccggaacca gcggcacggt
     1021 tgttgtaaac ggcatcgcgc ttgaaggcgt ctcgtccgac gaattttcgt acagcgtcat
     1081 cgagggcgcg gccgaggaat gggcgtccta ttcctcgatc agcctcgacg tcggtgaatc
     1141 cgccgttatt tcggtaacct acaccccgaa aaactcgggc accgacaacg gcaggatcgt
     1201 ctttgcacat aacgtcgcca cgcagggcaa caaaaccagc gtgaacatca cgacgtccgg
     1261 ccagaccggt gagttgacgg ccgaaccgtc accaatcgac ttcggcgaca tcaacgccgg
     1321 cgaggaagcc accctcgacg ttacaatcac gaacaacggt acgttcaccg ttgacgtgac
     1381 cgacgtctat ctggattacg agggaagttt cgacttcacg gttctcgatg tcacgtcaaa
     1441 ggacggcccg acgccggtaa ccgcgatggc accgggcgaa gtcctgaacg tcacgcttca
     1501 gtacaagcct gtcggaaccg gatacgacgg atccaacctg atcgtcctcg gaaacgtcga
     1561 agacaacctg cgccagtggt ccttcccggt cttcggttcc gaaatcggcc ccaggatcca
     1621 ggtcatcccg cagcagatcg aattcgattt cgttccgatt gacgaagaac gggaaagatc
     1681 aatcaccgtt tccaacatcg ggaacacgaa cctcagcatc acctccgtgg gaacgtcaat
     1741 gggatccagc ccggatctcg aaatcgtgaa cccgccggaa gtcggcgctc cagtcgtgat
     1801 caagccggaa cagaactacg aattcaaggt caagtggacg gcaaagaccc agtccgccaa
     1861 tccggaagcc ccaatcggcg cggtgacgat cgtaagcaac gatctttcga actccccgaa
     1921 tgttgttccg gtctatggcc aggtcgaggc cccctcgatc gaagttcttc cggcccagat
     1981 cgacttcggg ttcggcgcgc agaacaaggc gaagtcagcc ctgctgacga tcaagaacaa
     2041 cggcttcggc gcgcttacca tcagcaagct ggaaatcatc aacccctcga cgacccagta
     2101 cggacaggaa ttcgcgatca catacaacgc tggattcccc gttacgaact ctgtcaaccc
     2161 cgcgccaagc gacatcccgg ggaattcgtt caccccggtt gaactgacct
//
LOCUS       DAOPPA010000504         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53569_length_2207_cov_10.226766, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000504 DAOPPA010000000
VERSION     DAOPPA010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53569_length_2207_cov_10.226766"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>2207
                     /locus_tag="PKH54_12545"
     CDS             266..>2207
                     /locus_tag="PKH54_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOD00768.1"
                     /translation="MNRFLSVVSRFSMAVFVVGAVFFSACSDDENFGENLNLDYSPGT
                     INFGSVPIGGRKTITVTLTHIGDSGKVIIDSVTTQGLNSDYQAQMPAVAVLEPGDSTT
                     VDVTYVPTDSNTDSGYLVIGHNIATLEYKTLIAVTSAGQTAAIITEPSPIDFGEIPVG
                     EIATMDVVVKNIGTASFLIEQIYMDPEGPDSFNTIAIVPPEGETLPVELGPNETIGLT
                     IQFSPQEGKPEESLLYVTGEGNTWSFEVFGTGLGPKIQVNPTQLEMGGVNIGATGRAL
                     CVVTNVGNDTLRISNIAIAPGSDEGLSVEGDLDYNLAPGASRDVYVNWAVTEAKKFSG
                     NAIGWLSITSNDAASPTVVPIYGRVNTPILTVVPDPLVDMGYGAQGITVYRSVTVRND
                     GSADLVISGEPTLFEISEQKYGAEFRVEVIGATMSGGKYTLAAGAGMEVRISFTNKGP
                     ATGILTAKLKIISNDKSVPNTEKVLNIKVQRAGTATCIPVIEPNLYNFGMVPMGFTKE
                     LPVRVVNKGMGYCSYKGAQIQDCSGGFGGMTGTCSEPFTTTRSSKFLVESQPSVTLNG
                     MAPAGGTVFLRVKFAPPQTGSIFGDLTEYSALLGLMFRDSQLNKDLIIPERGTSGWTA
                     NLKGSSGVANLAVLPSDIKFGLV"
BASE COUNT          471 a          629 c          654 g          453 t
ORIGIN      
        1 gaacgtcacc ggtcaggcga tgtacaagtg cgacatgtgg aacgccgtcg agatctcctg
       61 gccccagcag acggtcacgc tgctgcgtaa tcctgacaat tccttgaaga ttaccaaggg
      121 atacgaggaa ccgtccttct ccgagatcgg cggtggaggt tgttccaccg agtagtcgcg
      181 cccgtcaaaa atcccgcctg attagttgtt tcaatttgca tcggaatcaa taaaataagt
      241 ccgttgcatt ctttgaggtt ttccgatgaa ccgttttttg tccgttgttt caaggttttc
      301 catggcagtg tttgtcgttg gcgctgtttt cttttcggct tgcagcgatg acgaaaactt
      361 tggcgagaat ctgaatcttg attacagccc gggtacgatc aacttcggct cggtgccgat
      421 cggcggccgg aagaccatca cggtaacctt gacgcacatc ggggattccg gaaaggtcat
      481 catcgattcg gtgacgacac agggattgaa tagcgactac caggcacaga tgcctgccgt
      541 ggcggtactc gaaccgggcg actcgacgac ggtcgacgtg acctatgtac cgacggacag
      601 caacaccgat tcgggctacc tggtcattgg acacaacatc gccacgcttg aatacaagac
      661 gctgatcgcg gtcacatcgg ccggacagac ggcggcgatc atcaccgagc cgtctccgat
      721 cgacttcggc gagattccgg ttggcgagat cgcgaccatg gacgtggtcg tgaagaacat
      781 cggcaccgca tcgttcctga tcgagcagat ttacatggat ccggaaggtc ctgattcttt
      841 caacaccatc gcaatcgttc cgccggaagg cgagactctt ccggtcgagc tgggcccgaa
      901 cgagaccatc ggtctgacca tccagttcag tccgcaggaa ggcaagcccg aggaatccct
      961 gctgtatgta accggcgagg gcaatacctg gagtttcgag gttttcggta ctgggcttgg
     1021 cccgaagatc caagtcaatc ctacgcagct cgagatgggt ggggtcaaca tcggcgccac
     1081 cggccgcgcg ctttgcgtgg tcacgaacgt cgggaacgac accctgcgga tctcgaacat
     1141 cgcaatcgct ccgggcagcg acgagggcct ttcggtcgag ggcgacctgg actacaacct
     1201 ggcacccggc gcttcgcggg acgtgtatgt gaactgggcc gtcaccgagg cgaagaagtt
     1261 ttcggggaac gcgatcggct ggctttccat caccagcaac gacgccgcca gccccactgt
     1321 cgtgccgata tacggccgcg tcaacacgcc gatcctgacc gtcgtgcccg atcctctggt
     1381 cgacatggga tacggcgccc agggaatcac ggtctacagg tccgtcacgg tcaggaacga
     1441 cggatcggcc gaccttgtga tatcgggtga gcccaccctt tttgagatct ccgagcagaa
     1501 gtatggcgcc gagttccggg ttgaagtgat tggagcgacc atgtcgggcg gtaaatacac
     1561 gctggccgcc ggcgcgggca tggaggttcg catttctttc acgaacaagg gacccgctac
     1621 gggcatcctc accgccaaac tgaagatcat ctccaacgac aagtcggttc cgaacacgga
     1681 aaaggtgctg aatatcaagg ttcagcgcgc cggcacggcc acctgcatac cggtaattga
     1741 acccaatctc tacaacttcg gcatggtgcc gatggggttc acgaaagagc ttccggtcag
     1801 ggttgtgaac aagggaatgg gctactgttc atacaagggt gcccagattc aggattgcag
     1861 cggcggattc ggcgggatga ccggaacatg ctccgagccg ttcacgacga ccagatccag
     1921 caagttcctg gtcgagtcgc agccgtcggt cacgttgaat ggaatggcgc ccgcgggtgg
     1981 aaccgtgttt ttgagggtaa aattcgcgcc gcctcagacc ggctccatct ttggcgacct
     2041 caccgagtac tcggccctgc ttggcctgat gttccgcgac agccagctta ataaggacct
     2101 gattatcccc gagcgcggca cttccggatg gacggccaat ctgaagggat cgagcggcgt
     2161 ggcgaacctg gccgttctgc cgtccgacat caagttcggg ctggtca
//
LOCUS       DAOPPA010000505         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53850_length_2198_cov_4.293514, whole genome shotgun sequence.
ACCESSION   DAOPPA010000505 DAOPPA010000000
VERSION     DAOPPA010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53850_length_2198_cov_4.293514"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PKH54_12550"
     CDS             <1..916
                     /locus_tag="PKH54_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD00769.1"
                     /translation="EKEPFVVRDIGAILSSKGFWLVALLCVLYYSAIFPFQKYAVNML
                     QCNLTFTEVPKESIWASTKIMLVQYGVMLIVAISAFMSNFQKRKAPRVALLVTAVALL
                     VVFCFMSYMRQSAESIFAVFPLLAVGITPIIGKYVDTKGKAASMLMIGSLLLIVCHLT
                     FAFVLPMFKGNNIGGMVLAFGTILVLGSSFSLVPASLWPSVPKLVDAKVIGSAYALIF
                     WVQNIGLWLFPLLIGKILTATNPDVATDLAAGKITPQVAAVSYNYTYPLLMLALLGVA
                     ALILGFVLKATDKKHGYGLELPNIATKE"
     gene            complement(966..>2198)
                     /locus_tag="PKH54_12555"
     CDS             complement(966..>2198)
                     /locus_tag="PKH54_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00770.1"
                     /translation="MKKLFDSGGNFMRLWMATWGFALEWTYPEGSVLGDYTPRMDRAW
                     ALDRVFWKAEALGLKVMLCMYSHGQFSTSSNSEWLNNPYNVVNGGPLPDAAAFFTDET
                     ARKLTRNRIRYIVARWGASPALMAWELFNEVDLTDQHDAATVAAWHQEMAALIKSLDP
                     YGRMVTSSISSFGTFWLMDQALFGDVPEIDLTQVHHYGSDLTKFDVAAEVPGMAESYS
                     RFGKPVFFGELGVHSAGPAESLAIDPSFIGLHDLIWAPVFAASAGSGMTWWWDNLIDP
                     NDQYFQFKAIAGFVSGIDWPGEGFEQAVLPTSDQQNLKAFAMIGTDTALVWVKNVAEK
                     YWQLGETADPAFIEGATLDLSGLPEGDWIVWWHDTFGTTSYPAEIEVVDNTETALPVP
                     GFSHDIALRLTRQKPAQR"
BASE COUNT          405 a          674 c          638 g          481 t
ORIGIN      
        1 ggaaaaggaa cctttcgtgg tgcgcgacat cggcgcaata ctttccagca agggcttctg
       61 gctggtcgcc ctgctttgcg ttctttatta ctcggcgatc tttccgttcc agaagtacgc
      121 cgtcaacatg cttcagtgca acctgacttt taccgaggtc ccgaaggaat cgatctgggc
      181 ctcgacgaag atcatgcttg tccagtacgg cgtcatgctg atcgtcgcca tcagcgcgtt
      241 catgagcaat ttccagaaga ggaaggcgcc aagagtcgcc ctgctggtta ccgccgtcgc
      301 cctgctggtc gtgttctgct tcatgtctta catgcgccag tccgccgaat cgattttcgc
      361 ggtgttcccg ctgctggccg tcggcatcac gccgatcatc ggcaagtacg tcgatacgaa
      421 gggcaaggcc gcatcaatgt tgatgatcgg ttccctgctg ctgatcgttt gccacctgac
      481 cttcgccttc gtgctgccga tgttcaaggg caacaatatc ggtggcatgg ttctggcgtt
      541 cggcacgatc cttgtgcttg gttcctcgtt ctcgctggtg cccgcgtccc tgtggccgag
      601 cgttcccaag ctggttgacg caaaggtcat cggctcggcg tacgccctga ttttctgggt
      661 ccagaacatc ggcctgtggc tgttcccgct gcttatcggc aagatcctga cggccaccaa
      721 ccccgacgtc gcgactgacc tggccgccgg aaagatcact ccgcaggtcg ccgccgtttc
      781 gtacaactac acctatccgc ttctgatgct ggccctgctc ggcgttgccg ccctgattct
      841 cgggtttgtc ctgaaggcca ccgacaagaa gcacggctac ggcctggaac tgcccaacat
      901 cgccacgaaa gaataactgc ggaaggcaac tgcgcgagtt tctccggcct gatggctccc
      961 tgagtctagc gttgtgcagg tttctgtctt gtcaggcgca gggcgatgtc gtgtgaaaag
     1021 cccgggacgg gaagggcggt ttcggtgtta tcgacgacct cgatttcggc cggatagctg
     1081 gttgtcccga aagtgtcgtg ccaccagacg atccagtcgc cttcgggcag tccggacaag
     1141 tcgagggtcg cgccctcgat gaacgccggg tcggcggtct cgccaagctg ccagtatttt
     1201 tccgccacgt ttttcaccca gacaagtgcg gtgtccgtgc cgatcatggc aaatgccttc
     1261 aggttctgct gatccgacgt cggcaggacg gcctgttcga agccttcgcc gggccagtcg
     1321 attccggaaa cgaaaccggc aatcgccttg aactggaagt attggtcgtt cgggtcaatc
     1381 aggttgtccc accaccaggt cattccggat ccggccgagg ccgcgaagac cggggcccag
     1441 atcaggtcgt gaagtccgat aaacgaagga tcgatggcca gggactcggc tggcccggcc
     1501 gaatgcacgc ccagttcacc gaagaacacc ggtttgccga atcgggaata gctttcggcc
     1561 atgccgggaa cctcggcggc cacgtcgaat ttcgtcaggt cgcttccgta gtggtgcacc
     1621 tgcgtcaggt cgatttccgg gacatcaccg aaaagcgcct gatccatcag ccagaacgtc
     1681 ccgaaggaag agatgctgct ggtcaccatg cgtccgtacg gatccaggga cttgataagg
     1741 gcggccattt cctggtgcca cgcagcgacc gtcgccgcgt cgtgctggtc tgtcagatcc
     1801 acttcgttga acagttccca tgccatcagg gcagggctgg cgccccagcg agccacgatg
     1861 taccggatcc ggttcctggt cagcttccgg gctgtttcgt ccgtgaagaa agccgcggcg
     1921 tcaggcagcg gaccgccatt gacgacgttg tacgggttgt ttaaccactc gctgttcgac
     1981 gacgtcgaaa actgtccgtg gctgtacatg cacaacatca ctttgagtcc aagcgcctcg
     2041 gccttccaga acacccggtc aagtgcccag gcgcggtcca tcctgggcgt gtagtcgcca
     2101 agcaccgaac cttccgggta agtccattcc agggcgaatc cccaagtcgc catccacagg
     2161 cgcatgaagt tgccgccgct gtcgaaaagc ttcttcat
//
LOCUS       DAOPPA010000506         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53889_length_2197_cov_3.711951, whole genome shotgun sequence.
ACCESSION   DAOPPA010000506 DAOPPA010000000
VERSION     DAOPPA010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53889_length_2197_cov_3.711951"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PKH54_12560"
     CDS             <1..1451
                     /locus_tag="PKH54_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOD00771.1"
                     /translation="EDAGTVSFQLSYAAVFGIMLAGRLLPAQGRRKGFWPRAGRFVAS
                     SLMVSVGASLATIPLTVIYFGSAPVVGPLANLVVVPMFTFVGLPGAFLTIILLGSGTT
                     VGTGPAGVAIWLYCHAVDFMMAIQEWTSSRLPAILAWPPWPMDALAAACCTGAAVFLA
                     LSRTRAAAAMALLAAAAILLIPTWPRPITGQVVFLDVGKGDAAVLRCPSGSIWLVDAG
                     APEKGRSLLADGLNRNRVRHIDGVVVTHAHDDHYGGIPMLLDRFGPLDLAASRSTLAG
                     MPQVVAAVRRAGGSIVELSPGGQAVPGCGIDTTVLAADLATAGPAHENCERSKNVNDD
                     SLVFRFGKGANSILFAGDLETPGEKCLLALGVAPQAGILKVGHHGSPGASSPDFVSSV
                     SPLTAIITGWPNGPRKAPSDIVLSRFLQSGSKVMATTLLGDVIVELAHGKNEKMPSSP
                     VLKPQKAFPDGFGGRDGQARAMLKMIESPDKP"
     gene            1690..>2197
                     /locus_tag="PKH54_12565"
     CDS             1690..>2197
                     /locus_tag="PKH54_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00772.1"
                     /translation="MGSKSKEPHFYGMFEAAMDNKTGRFIVPTVVYRPLGDRVRFTVD
                     PLGRFLEVRSEDDFQALVDRINQAEASLPVEVITALRMDYLGFSSEGQIDGSLRLVVP
                     KRMRDVLADEDDLVLVAVKDCLQVWGARRYRAERQARMKEFAGRYQDVQHLIMGIARP
                     EPAAFPVVA"
BASE COUNT          417 a          638 c          686 g          456 t
ORIGIN      
        1 cggaagacgc cggcacagtc tcgttccagc tttcatacgc ggcggtcttc ggaatcatgc
       61 tcgccggacg cctgcttcca gcgcaagggc gccgaaaggg cttctggcca agggcaggcc
      121 ggtttgtcgc atcgtcactg atggtatcgg tcggggcctc gctggcgacg atcccgctga
      181 cggtcatcta tttcggatcc gctccggtcg tcgggccgct ggccaatctt gtggtggttc
      241 cgatgtttac gttcgtggga cttcccggag ccttcctgac aatcatcctg cttggatcgg
      301 gaacgacagt cggaaccggg ccggccgggg ttgcgatatg gctgtactgc cacgcggtcg
      361 acttcatgat ggccatccag gaatggacct catcgcgcct gccggccatc ctggcatggc
      421 caccatggcc gatggacgcg ctggcggccg cctgctgcac cggcgccgca gtgttcctgg
      481 cgctttccag gacaagggcc gcggcggcaa tggcgctgct tgcagccgct gcgattctgc
      541 tgattccaac ctggccccgt ccgatcaccg gccaggtcgt attccttgac gtcggaaagg
      601 gggacgcagc cgtcttgcgc tgtccctccg gatcgatctg gctcgtcgac gccggtgcgc
      661 ctgaaaaagg aagatccctc cttgccgacg ggctgaacag gaaccgcgta aggcatatcg
      721 acggagtcgt ggtcacgcac gcccatgatg accactacgg cggcattccg atgctgcttg
      781 atcgcttcgg tccgctggat ctggctgcgt ccaggtcaac ccttgcggga atgccgcaag
      841 tcgtcgcggc cgtcaggcgg gccggcggca gcatcgtcga actgagcccc ggcgggcagg
      901 cagttcccgg ctgcggaatc gacaccaccg tgctggcggc cgatcttgcg accgccgggc
      961 ccgcgcacga aaactgtgaa aggtcaaaaa acgtgaatga cgactcgctg gtgttccgat
     1021 tcggcaaagg agcgaattcg atcctgttcg ccggggatct tgagacgcca ggggaaaaat
     1081 gtcttcttgc gcttggggtt gcgccgcagg ccgggattct taaggtcgga catcacggaa
     1141 gcccgggggc aagcagcccc gacttcgtgt ccagcgtgtc gccattgacc gccatcataa
     1201 ccggatggcc caacggcccg aggaaggccc cgtcggatat cgtgctgtca aggtttcttc
     1261 agtctggatc aaaggttatg gccacaacac tgctcgggga cgtgattgtt gagcttgcgc
     1321 acggaaaaaa tgaaaaaatg ccgtcttccc cagtgctgaa gcctcaaaag gccttccctg
     1381 acggttttgg cggcagggat ggtcaggcaa gggcaatgct caaaatgata gaaagtcccg
     1441 ataagccttg ataaatcggt ctccaatcgt ccttgtgcaa tcgcagtgac tctttggtcg
     1501 aagcaggcga cagcaggcct tgccgaaatt ggactgaact agtccgtgta taagccaccg
     1561 ccataacata ttgatttaat tggatgaaaa attcatctgg cgaacaatgg ctgtggggtg
     1621 ttgccaagtg gttccctggg tagcatcatt ggttgttgaa gtaaccttat tggatcccgg
     1681 gggtacccta tgggctcaaa gagcaaggaa ccgcacttct acggaatgtt cgaagcggcc
     1741 atggacaaca agaccggccg cttcatcgtc ccgacagtcg tatatcgccc tctcggcgat
     1801 cgggttcgtt tcaccgtcga tcctctcgga cgctttcttg aggtccgttc cgaggacgac
     1861 ttccaggcac ttgtcgatcg gatcaaccag gccgaggcaa gtcttccggt ggaagtcatc
     1921 acggccctca ggatggatta tctcgggttt tcgtcagagg gccaaataga cggcagtctt
     1981 cggctggttg ttccgaagcg gatgagggac gtcctggccg atgaggacga ccttgtgctg
     2041 gtcgccgtca aggattgcct gcaggtttgg ggagcccgtc gctaccgtgc cgaacgccag
     2101 gcccgcatga aggaattcgc cggccggtac caggatgtgc agcacctgat tatggggatt
     2161 gccaggccgg agccggccgc cttcccggtg gtcgcga
//
LOCUS       DAOPPA010000507         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_53975_length_2195_cov_4.100467, whole genome shotgun sequence.
ACCESSION   DAOPPA010000507 DAOPPA010000000
VERSION     DAOPPA010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53975_length_2195_cov_4.100467"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKH54_12570"
     CDS             complement(<1..554)
                     /locus_tag="PKH54_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00773.1"
                     /translation="MTVTSSLESFDVTRVGPGMRDPSGFAGAMPPSYFDHVGPEFHAQ
                     HELFLDYFRGDSGEGRVFSFQGDSELFILHNFDAESIRRHLGGAVKSLRDRPFRYRSF
                     VNNRERFVIHQICSDFDDAPGGTTGRRKGADTCPDLGFSDCRGQYLDDQGRVFRHVAV
                     SYDEKSGSFLLNCVNVAHQTLLSNL"
     gene            706..>2195
                     /locus_tag="PKH54_12575"
     CDS             706..>2195
                     /locus_tag="PKH54_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD00774.1"
                     /translation="MHRTIVGLVTMKKSTKMSGSTQSPGQADEADRQVTALEEQTARL
                     QAILDTSVVAIVSIDEHGIIEMVNRATTDMFGWEEGSMIGRNVSIIVPPPHAEMHDGY
                     IRAYLQTGIRKVLGHAREVMARRRDGTLIPIEITLSETRFGDHHHFTAVIRDISARKA
                     ATRELEAAMKRLTRANEYLRAVLNRLRVGTLLVDHDGMVDYFSDMCAPLLARNEPGAV
                     HEWTDFFRVGERLQATLRDAMKTPEPARGRLQIHLPFGQEQRWFEIDIRDDIDDPSRR
                     QVFLYDIDEIVGLRDSVRQDRYGPIIGRSDAVRSMVRMIERVASGEWTVMIEGPTGSG
                     KELAARAIHEASPRRGGPFVAVNCAGLADSLLASQLFGHRKGAFTGAVADQQGFFEAA
                     NGGTLFLDEIGDISPQLQASLLRVLQEKEILRVGDTRPRKVDVRVLVATNLDLAAEVT
                     AGRFRQDLLYRIRVARIAVPSLKERREDIPLLARHFMDENANQVDSA"
BASE COUNT          501 a          669 c          632 g          393 t
ORIGIN      
        1 agattcgaaa gcagcgtctg gtgggccaca ttcacacagt tcagcaggaa agatccggac
       61 ttttcgtcat aactgactgc aacatgacgg aacacgcgcc cctgatcgtc aagatactgc
      121 ccgcggcaat cactgaaccc aaggtcggga cacgtgtcgg caccctttcg ccttccggtc
      181 gtcccaccgg gagcatcatc gaaatcggaa catatctgat gaatgacgaa cctttcgcga
      241 ttgttcacga aactgcggta tcgaaacggc cggtcgcgca gcgacttcac cgccccgccc
      301 aggtgccggc ggatcgattc ggcatcgaaa ttgtgaagga tgaacagttc actgtcgccc
      361 tggaaactga acacccggcc ttcgccggaa tcgcccctga agtagtcaag aaacagttcg
      421 tgctgggcat ggaattccgg cccgacatgg tcaaaatacg acggcggcat cgccccggcg
      481 aaaccggacg gatcgcgcat cccggggccg accctcgtga catcgaaaga ttccagactg
      541 ctcgtaactg tcatggcctc atcaaatccg gaaagcccgg cgtccctctg gcgacgcgta
      601 atgatacaac ggctttggcc tttcgcgctg gacaacagga acacgccccg ccagacacgc
      661 atcgcttaac ggaaattggg cattgaacgt gtcgcggcta tacttgtgca cagaacgatt
      721 gtcggactgg tgacgatgaa gaaatccacg aagatgtccg gctcgacgca atcaccggga
      781 caggccgatg aagccgaccg ccaggtgacg gcactcgagg aacagacggc cagactccag
      841 gcgatcctcg acacttcggt cgtcgccatc gtctcgattg acgaacacgg catcatcgaa
      901 atggtcaacc gggccacgac cgacatgttc ggctgggaag aaggctcgat gatcggccgt
      961 aacgtctcga taatcgttcc gccgccacac gcggaaatgc acgatggata catccgcgcg
     1021 tatctgcaga ccggaatcag gaaggttctg gggcacgcca gggaagtgat ggcccgccgt
     1081 cgcgacggca cccttattcc catcgaaatc accctcagcg aaacaaggtt cggcgatcat
     1141 caccacttca ccgccgtcat ccgggacatt tccgccagaa aagcggccac gcgcgagctt
     1201 gaggccgcca tgaagcggct gacaagggca aacgagtacc ttcgggccgt tctcaaccgc
     1261 ctgcgggtgg gaaccctcct ggtcgatcac gacggaatgg tcgattactt ctcggacatg
     1321 tgcgccccgc ttcttgccag gaatgaacca ggcgccgtgc atgaatggac cgacttcttc
     1381 cgcgttgggg aacgactcca ggccacgctt cgggatgcga tgaagacgcc cgagccggcc
     1441 aggggaagac tccagatcca ccttcccttc ggccaggagc agcggtggtt cgaaatcgat
     1501 atccgggacg acatcgacga cccttcaaga cgccaggttt tcctttacga catcgatgaa
     1561 atcgtcggcc tgcgcgattc ggttcgccag gacaggtacg gtccgataat cggaagaagc
     1621 gatgccgtcc ggtcgatggt caggatgatt gaacgcgttg cgtccggcga atggactgta
     1681 atgatcgaag gaccgaccgg atccggaaag gaacttgccg caagggccat ccatgaagca
     1741 agtccaaggc gcggcggccc gttcgtcgcc gtcaactgtg ccggcctggc ggactcgctt
     1801 ctcgcgagcc agcttttcgg acaccgcaaa ggggccttca ccggcgctgt cgccgaccag
     1861 cagggattct tcgaggcggc aaacggcgga acccttttcc tcgacgaaat cggcgacatc
     1921 tccccgcaac tccaggcgtc cctgctgcgg gtcctgcagg aaaaggaaat actgcgtgtc
     1981 ggggacacca ggccaaggaa agttgacgtc cgcgtgcttg tggcgaccaa cctggatctt
     2041 gctgccgaag tgacggccgg ccgattccgc caggatctgc tttaccgcat cagggttgca
     2101 aggattgccg ttccttccct gaaggaaaga cgcgaggaca tcccacttct ggcaagacat
     2161 ttcatggatg aaaacgccaa ccaggttgat tcggc
//
LOCUS       DAOPPA010000508         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_54039_length_2193_cov_3.761459, whole genome shotgun sequence.
ACCESSION   DAOPPA010000508 DAOPPA010000000
VERSION     DAOPPA010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54039_length_2193_cov_3.761459"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1120
                     /locus_tag="PKH54_12580"
     CDS             47..1120
                     /locus_tag="PKH54_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOD00775.1"
                     /translation="MRVMTVTATAALMMIVVPGCGGQDEPWLPDVAAVGDGGVEAGEP
                     DVLEQIDVPVPRDEGQDHGAPDPGEPGADEGQPVNTGLYEDGMFPDLASCEPGQNTEY
                     QKELALDRLNYVRSLHGLPEVWYDDSGDAEVAACSLTCVSKRMLSHYPDENWPCYTQL
                     AADGCGSSNLHLQYGKAPVGYPTAGIVDGFIRDENVDAVGHRRWFLDPWLQKTAFARV
                     DDFQGKYTAASMKVIWKGSGSGSVSMEDGYVAYPFHDYPEALYTDKAHMSFSVIADKS
                     SKWKNGDENVRFTSPAVTVKEEGGASIAVSDIGYDTQGYGVPNCLWFKAGPTKKGVRY
                     DVTVSGITVNGKPMIYQYWFRRF"
     gene            1266..>2193
                     /locus_tag="PKH54_12585"
     CDS             1266..>2193
                     /locus_tag="PKH54_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00776.1"
                     /translation="MADSFFELSAVANNGLIGRFSAVVVILAAVGMSGCTESNRPPSA
                     LAPAGSSESLVGRNPDVVFNAGFVFPGGWTVESVRRHPEFIRLVLVGQDTTTGLEIIP
                     GTGTPTRFCSQVYCVQPAPGHSPPAELLLIAVSYLPRQPVEGFPKATIRPATKIRNAV
                     MATLTSEALLISLAVALGVLILPVLFSAFSVSGVGWRRGLLFVVGACVVFCGVWYFTL
                     SDPDYLATRLTTLQDGTTHQNLLHLYGQGVHSSPFSQALAGLVRDDPVLVTLRHVARF
                     NLWLTAFNVALLFLVAAFVLNSIVLAAPLAAAF"
BASE COUNT          400 a          621 c          660 g          512 t
ORIGIN      
        1 catcgaatat aatgcaggcc gacagttgcg acctgcgggg atatgcatgc gagttatgac
       61 agtgacggcc accgcggcct tgatgatgat tgtggttccg ggttgcgggg ggcaggatga
      121 gccctggctt ccggacgtcg cggccgtcgg tgatggcggc gtggaagccg gggaacccga
      181 tgtccttgaa cagattgatg ttccagtccc gcgggatgaa ggccaggatc atggcgcgcc
      241 ggatccgggc gagccgggcg cggacgaagg tcagccagtc aataccgggc tttacgagga
      301 cggaatgttt cccgatctgg catcctgcga gcctggccag aacacggaat accagaagga
      361 acttgccctt gatcgcctga actacgtgag atccctgcac ggcctgcccg aggtctggta
      421 tgacgattcg ggtgatgccg aggtcgcggc ctgttccctt acctgtgttt caaaaaggat
      481 gctcagccac tatccggacg agaactggcc ctgctatacg caactggccg cggacggatg
      541 cggcagcagc aacctgcacc tgcagtatgg aaaggccccg gtcggttatc cgaccgccgg
      601 catcgtcgat ggattcatcc gtgatgagaa tgtggatgcg gtcgggcatc gtcgatggtt
      661 cctggatccg tggcttcaga agacggcgtt cgcgcgtgtc gatgatttcc agggcaagta
      721 caccgccgca tcgatgaagg tcatctggaa aggctccggc agcggttcgg tcagcatgga
      781 agacggctat gtcgcctatc ctttccacga ttaccctgaa gcgctttata ccgacaaggc
      841 acacatgtcg ttttcggtaa tcgccgacaa gtcttcaaaa tggaagaacg gggatgaaaa
      901 cgtgcggttc acgtcgcccg ccgtcaccgt caaggaagag ggaggtgctt caatcgcggt
      961 ttccgatatc ggttacgaca cccagggata cggtgtcccg aactgtctct ggttcaaggc
     1021 gggacccacg aaaaagggtg tgcggtatga tgtcacggtc tcgggtatca cggtgaacgg
     1081 caagccgatg atctatcagt actggttccg acgcttctga tccgtcgttt cgagtgcgaa
     1141 tccttgattt acaattactt tgccaaagga gtgttgacgg cgctcacgcc aggccccaag
     1201 ctgtaacagg ctttgatacc gacccgaatt tcattcgttt tcaaattccg tgggaggcag
     1261 tgaaaatggc ggactcattt ttcgaattat cggcggttgc caacaacggc ctgatcggac
     1321 ggttcagtgc cgtggtcgtc attcttgctg ccgtcggcat gtccggctgc acggagtcga
     1381 accgcccgcc atcggccctc gcgcctgcgg gttccagcga gtcgcttgtc gggcgcaatc
     1441 cggacgtcgt gttcaacgcc gggtttgttt ttcccggcgg atggacggtc gaatcagtcc
     1501 ggcgccatcc agaattcatc aggctggttc tcgtcggtca ggacaccacg acagggcttg
     1561 aaatcattcc tggcacggga acgccgacgc ggttctgttc ccaggtttac tgcgtccagc
     1621 cagctcccgg ccattcgccc cctgccgagc ttcttctaat tgcagtgtca tatcttccca
     1681 ggcaacctgt cgaaggattt ccgaaggcaa ccatcaggcc ggccacgaag attcgcaatg
     1741 ccgtcatggc gaccctgaca tctgaagcgt tgctgatttc gcttgccgtc gcgttgggcg
     1801 tccttatttt accggtccta ttttcggcct tttccgtttc gggagtcggg tggcgacgcg
     1861 gccttctttt tgtcgtcggc gcatgcgtcg ttttctgcgg tgtatggtat ttcacccttt
     1921 ccgatccgga ctacctggcg acaaggctta cgacgctcca ggacgggacg acccaccaga
     1981 atctgttgca cctgtacggt cagggagtgc attccagccc gttttcccag gcgctggcgg
     2041 gtcttgtgcg ggatgatccg gttctcgtga cgcttcgtca tgtcgcccgc ttcaacctgt
     2101 ggttgacggc cttcaacgtg gccctgctgt ttcttgtcgc cgcgttcgtg ctgaattcga
     2161 ttgttcttgc ggctcccctg gcggccgcct ttt
//
LOCUS       DAOPPA010000509         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_54042_length_2193_cov_3.530870, whole genome shotgun sequence.
ACCESSION   DAOPPA010000509 DAOPPA010000000
VERSION     DAOPPA010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54042_length_2193_cov_3.530870"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKH54_12590"
     CDS             <1..941
                     /locus_tag="PKH54_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00777.1"
                     /translation="DFINPITFGEYIDRFGPGRPLHRLATGSWIDGNFDTWIGEPTKN
                     RAWDMLATARQAVAASDMKARNRESVEAHLLRAEASDWFWWFGQGHSSIHEREFDFLF
                     RRNLRAVYEDAGLQAPACLDRPVSEESTGLPVTMPSAMISPRITGRRDSYYKWLGSGI
                     CSFSHGPIHRLQPLIGAVRFGFDRESIFLAAEKFAGANGFIQPGSTVRVVFESPRKLT
                     VSLMLNENGNFSISAFDGDGNGIDAEKVAAASLDVLEVRIPQSLLFERDREGAKAVSF
                     FVEIESGGVDQERFPWDSSIEFGWDSRTFELQGWFV"
     gene            975..2006
                     /gene="galT"
                     /locus_tag="PKH54_12595"
     CDS             975..2006
                     /gene="galT"
                     /locus_tag="PKH54_12595"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOD00778.1"
                     /translation="MPELRHDPVQKRWVIIATERARRPQDFVFSQPMDNGHDCPFCAG
                     NEDATPPELCRINYPDGTWKARCIPNKFPALRVEGELERAAVGQYDRMNGIGAHEVIV
                     ESPDHNKDLPDLGVEEIAAVLRIYRERLIDLSRDIRLKYILIFRNHGARAGASLAHPH
                     SQLIATPITPRTVALELESARQHYQVKERCIFCDIIAQEMQEHRRLVLVDHDFVTWCP
                     YASRFPFEMMLAPRRHNHDFSALTDADLLKLAGHLRNVLRRMKASLEDPAYNFLIHTA
                     PAYHMQNQRSGWGSTITLDWHWHIEILPKLSNAAGFEWGTGFYINPTPPEDAAAFLRE
                     VCLDERAQC"
BASE COUNT          458 a          666 c          657 g          412 t
ORIGIN      
        1 cggacttcat caacccgatt accttcggcg agtacatcga caggttcggg ccgggccggc
       61 cccttcaccg gcttgccacc ggctcgtgga tcgacggtaa cttcgataca tggattgggg
      121 agccgacaaa aaaccgggca tgggacatgc tggcaaccgc caggcaagcc gtcgcggcat
      181 ccgatatgaa ggcacggaat cgcgaaagcg ttgaggccca cctgttgcgg gccgaggcat
      241 ccgactggtt ctggtggttc ggacaaggac attcttccat ccacgaacgt gaattcgact
      301 tcctgtttcg gcgcaacctg cgcgcggttt atgaggacgc tggcctgcag gcgccggctt
      361 gccttgacag gcccgtatcg gaagaatcga ccgggctgcc cgttacgatg ccttcggcga
      421 tgatttcacc caggattacc ggcaggcgcg acagttacta caagtggctg ggatccggga
      481 tctgcagctt cagccacggg ccaatccaca gactgcaacc gctgatcggg gccgtcaggt
      541 tcggattcga ccgggagtcc atcttcctgg cggcagagaa gttcgccggg gccaacggat
      601 tcatccagcc gggatcgacg gtgcgcgtcg tgttcgaatc gccgcgcaag ctgaccgttt
      661 ccctgatgct gaacgaaaac ggaaacttct cgatttcagc gttcgacggc gatgggaacg
      721 ggattgacgc cgaaaaggtg gccgcagcct ctctcgacgt cctggaagtc cggattccac
      781 agtcactgct ttttgagcgc gatcgcgagg gcgcaaaggc tgtatcattc tttgttgaaa
      841 tcgagtccgg cggcgttgac caggaacgct ttccgtggga ctcgtcgatc gaattcgggt
      901 gggactcccg cacatttgaa ctgcagggct ggttcgtctg actggaagtc gacccgcctg
      961 gcgagggatc actaatgccg gaactcagac acgacccggt tcagaagcgc tgggtcatca
     1021 tcgcgacgga aagggcccgc aggccacagg atttcgtctt ctcccagccg atggacaacg
     1081 ggcacgactg tccattctgc gctggaaacg aagacgcgac gccacctgag ctctgccgga
     1141 tcaattatcc ggacggaaca tggaaggcgc gctgcatccc gaacaaattc ccggctctgc
     1201 gcgtcgaggg cgaactcgaa cgggccgccg tcggacagta cgacaggatg aacggcatcg
     1261 gcgcgcatga agtcatcgtt gaatcacccg atcacaacaa ggacctgccg gatctcggcg
     1321 tcgaggagat cgcggccgtc cttcggatct accgggaacg actgattgat ctttcccgcg
     1381 acatccgcct gaagtacatc ctgatcttcc gtaaccacgg cgcaagggcc ggcgcctctc
     1441 tggcacatcc gcactcgcag cttatcgcga ccccgataac gccccgcacg gtcgcgctgg
     1501 aactggaaag cgcgcgtcag cattaccagg taaaggagcg ttgcatcttc tgcgatatca
     1561 tcgcccagga gatgcaggaa cacaggcgcc tggtgctggt cgatcacgac ttcgtgacct
     1621 ggtgtccgta cgcgtccagg ttcccgtttg agatgatgct tgctccccgc cggcacaacc
     1681 acgatttttc ggcgctgacg gacgcggatc tcctgaagct cgccggacac ctgcggaacg
     1741 ttctccgccg catgaaggcc agcctcgaag atccggcgta caatttcctg atacacaccg
     1801 cgccggccta tcacatgcag aaccagcgat ccggctgggg atcaacgatt acgctggact
     1861 ggcactggca catcgaaatc ctgccgaaac tttcaaacgc ggccggattc gaatggggaa
     1921 ccggattcta catcaacccg actccacctg aagacgcggc ggccttcctg cgggaagtct
     1981 gcctggacga acgggcgcag tgctgattct gcttgcgggg cgcgatgaaa cggcacgttg
     2041 acataaaact gcactacgcg tgcaacaaca actgcatcca ctgcgtgatt gccgatcagc
     2101 gcaagaccgt gcttgaacgc ggcgaccggg atttcagaac cacggccgag gtcgttgcgg
     2161 aacttgccga tgcaagggcg cgcggtttcg atg
//
LOCUS       DAOPPA010000510         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_54184_length_2188_cov_5.335209, whole genome shotgun sequence.
ACCESSION   DAOPPA010000510 DAOPPA010000000
VERSION     DAOPPA010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54184_length_2188_cov_5.335209"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..1155
                     /locus_tag="PKH54_12600"
     CDS             571..1155
                     /locus_tag="PKH54_12600"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOD00779.1"
                     /translation="MARDKFDSVRELLYWSYANLAMAHTAVERRQARYGTFNYMIRAK
                     LFKGLMDRTMHIRSLFDDEKVKLVSGNRCSYCGSTLKLSVDHVFCQKMGGKDSGDNLI
                     YACRTCNSSKGKKDLMEWMHHRNSFPPLMILRRYLKLVVCYCEDNGLMDCAVDDLKGR
                     ELPFRIELIPIDYPDPAELVMAVDVVPSANVREK"
     gene            complement(1215..>2188)
                     /locus_tag="PKH54_12605"
     CDS             complement(1215..>2188)
                     /locus_tag="PKH54_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00780.1"
                     /translation="SFTSVSVDSESLTKNSDETRIVAFRGQESSGSLKVGGATWNAPF
                     ATSTSFAGQQECTPGDPPPTAIPAANPWEPGPHAAGFADFNGGAFGVKVRVYYPATCG
                     GLRKPPADGKFPFVYFVHGDGYIPLNFEYLARHLATWGFMSAIPDNPGYSSFNTGRTS
                     PKTWFGALAGHETGSDAIIVCHSKGAEYTYNLGLANVAAVVFLGPVYTMEDPYGSLFP
                     IQGLVVGWSTDGSMNTDKCYDVYQQLQAPKYAAMIIGGTHGHFLDDKMWDPPSDGSGD
                     YITRNRQHELTQAFVLPYIQHIFKTAEPFAEILSSPGLEGEITFTSK"
BASE COUNT          444 a          582 c          649 g          513 t
ORIGIN      
        1 ttagacggac tgaaaatccg cgtgtcggtg gttcaatccc gcccctgggc acaaatagaa
       61 tattccagtc tcttccatgt cggtgccgat gtatcgcggt ctgcacctgc cagccggttg
      121 ccccccccag atccgtaagg gcgaatgaaa attcgcccgc ctgcgtgaaa attcgcccgc
      181 ctgcgtgaaa attcgcccgt gggcccttcg cttcgctcag gggttcagga gctgccgtac
      241 gccccttgtt ccttgccact gggggtcctt cggggttacc cgtcccggga atcgcggtcg
      301 cgcacggggc cagccccgtg tcgcgccgct gcctctcggc gcgcgacggt cattacgccc
      361 cgcaaggtgt cgcgcgcctg cgagagcttc ccgggtacgg gtacccgctc ggcccccatg
      421 tcgcccgccc cctgtttcag ttgttttggc ggaacgaagt gagcgtgaag ctttgcagcc
      481 agttcaatcg attcgccgtg aaaatcatca gatccgattt gagtgaaatc gtggcatgat
      541 ttgattccga cagccacacg atggtcgcat atggccaggg acaaattcga ttctgtcaga
      601 gagctccttt actggtccta tgcgaatctg gccatggcac atactgcggt tgagcgaagg
      661 caggccagat acggaacctt caactacatg ataagggcca agcttttcaa gggattgatg
      721 gacagaacaa tgcatattcg aagcttgttt gacgatgaaa aggtcaaatt ggtttccgga
      781 aatagatgca gctattgcgg ttcaacgctc aagctttcgg ttgatcatgt tttttgtcag
      841 aaaatgggtg gcaaggattc aggcgataat ctcatctatg cctgccgaac ctgtaatagt
      901 tcgaaaggga agaaggactt gatggaatgg atgcatcaca gaaattcatt tccgcctttg
      961 atgatcctga ggcgttattt gaaactcgtc gtttgttact gtgaagacaa cggccttatg
     1021 gactgcgcgg ttgatgatct taagggacgg gaattgccct tcaggattga attgattcca
     1081 attgattatc cagatccggc agaactggtc atggctgttg atgtggttcc gtcggccaac
     1141 gttcgggaaa agtgaatccg cgtggcggtg gttcaatccc gcccctgggc acaaatcaac
     1201 acgtatcgtc ttcgtcattt gctggtaaaa gtgatctcgc cttccaggcc tggacttgaa
     1261 agtatttccg cgaatggttc ggccgtcttg aatatgtgct ggatgtacgg caggacgaag
     1321 gcctgcgtca gttcgtgctg gcggttcctg gtgatgtagt cgccgctgcc gtcggaagga
     1381 ggatcccaca tcttgtcgtc caggaaatga ccgtgtgttc caccgataat catcgcggca
     1441 tatttcgggg cctgcagttg ctggtaaacg tcataacact tgtcggtgtt catgcttccg
     1501 tcggtggacc atcccaccac aaggccctgg attgggaaca ggctgccgta cgggtcttcc
     1561 atggtgtaaa cggggcccag gaaaacgacg gcggcgacgt tcgcaaggcc aaggttgtac
     1621 gtgtattcag cgccttttga gtgacagacg atgattgcgt ccgagccggt ttcatggccc
     1681 gccagcgcgc cgaaccaggt tttgggggac gttcgcccgg tgttgaagct tgaatagccc
     1741 gggttgtcgg gtatggccga catgaagccc caggtggcca ggtgtcttgc gagatactcg
     1801 aagttcaggg ggatatatcc gtcgccgtgg acgaaataca cgaacgggaa ttttccgtcg
     1861 gccggaggct tgcgcagccc accgcaggtg gccggataat agacgcgaac cttgaccccg
     1921 aacgccccgc cgttgaagtc ggcgaatccg gcagcgtgcg ggccgggttc ccaagggttg
     1981 gctgccggga ttgccgtggg cggcgggtcg cctggcgtgc actcctgctg gccggcgaaa
     2041 gacgtcgatg tcgcgaacgg cgcattccag gtcgcgccgc cgactttcag gcttccgctg
     2101 gattcctggc cgcggaacgc aacgattctt gtttcatccg aatttttcgt cagagattct
     2161 gaatcgaccg ataccgacgt gaaagaat
//
LOCUS       DAOPPA010000511         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_54770_length_2170_cov_3.782506, whole genome shotgun sequence.
ACCESSION   DAOPPA010000511 DAOPPA010000000
VERSION     DAOPPA010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54770_length_2170_cov_3.782506"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKH54_12610"
     CDS             <1..556
                     /locus_tag="PKH54_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00781.1"
                     /translation="VGVVAALGVAVGGITAAFGALMQAFFGLGVWLPLGLIGIILLIS
                     GPSMAIAALKLRQRNLGPLLDANGWAINAKARVNIAFGNGLTRVAELPAGATCSLVDP
                     YAKKKRPKWPWIVLLVVVLLVGGGLFAWNKGWLNDWLPDSLDRNLPKAVETVPAPAAP
                     AAQPAPAAPAAQPAPTSAPAATAG"
     gene            571..>2170
                     /locus_tag="PKH54_12615"
     CDS             571..>2170
                     /locus_tag="PKH54_12615"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOD00782.1"
                     /translation="MSLHIVFGSDTARLAHRLASDIAQSTSGAGLAGLLMPTSVLVPN
                     RNMEKWLRFEIARHNGIAANLSFPYLENGLWELVGRIGVASGRVAADRSGRPILDRLD
                     LETLTARLAAILLQDTADPSRIDSSFVSYCMAGGGPGDPGFSIRLWQLAGRLAHLFRE
                     YEFNRSGMVREWLAGKIDPSAGELHARQAGLYRQACSLLYYDWDAHAARPARSLFQLG
                     EEVFQDPGHRHGIRNDEPDWGETAGEVAPPAGTLPGTVFIFGLSTLSRYHCRRLWEIS
                     RRCDIRLYHVNGCHEFWQDVSTPFEERWHRIRAARVVSVPDGQGQELALDNDLDDNLL
                     LKAWGKAGRETVRLLSELEDQPSGVTTEWVDEPEIDFDRATVLDRVRHGIRTRSSQVG
                     RTVADDSLVVLKCPGRLREVEAVAERITALLQADPDLKLSDIVVLVTDMASYKPLISQ
                     VFDRDRSRPDNVWDPIPYNLADSTAGTDSVYAAAVEAMLNLAGGDFSRGAVFRLLFNP
                     CFMAAAGVGPATVEGWAGLADRLGI"
BASE COUNT          357 a          662 c          727 g          424 t
ORIGIN      
        1 tgtcggcgtg gtcgccgccc tgggcgtcgc cgtcggcggc atcacggcgg ccttcggggc
       61 gttgatgcag gcgttctttg gacttggcgt ctggctgccg cttggcctga tcgggattat
      121 cctgctgatt tcggggcctt ccatggcaat tgccgcgttg aagctgcgcc agagaaacct
      181 tgggccgctg cttgatgcga acggatgggc gattaacgcc aaggcgcgcg tcaacatcgc
      241 gttcggaaac ggccttacaa gggtcgcgga actgccggcc ggggcgacct gctcgctggt
      301 tgatccttac gcgaagaaga aacgtccgaa gtggccctgg atcgttctgc tggttgtcgt
      361 ccttctggtc ggcggcggtc tgtttgcctg gaataagggc tggctgaacg actggctgcc
      421 cgactcgctg gatcgcaacc tgccgaaggc ggtcgagacg gttccggccc ccgcggcccc
      481 ggccgcgcag cccgcgccag cggcccccgc ggcacagccg gcaccgacat ctgcacctgc
      541 ggccacggcc gggtaacccg ggggttcccg atgtccctgc acatagtttt cggaagcgac
      601 accgccaggc tggcgcaccg tcttgccagc gacatcgccc agtccacgtc cggcgccggg
      661 ctggccggcc tgttgatgcc gacaagcgtg ctggtgccga accgcaacat ggaaaagtgg
      721 ctgcgcttcg aaattgcaag gcacaacggc atcgcggcga acctgtcgtt tccgtacctg
      781 gaaaacggcc tgtgggaact ggtcggacga atcggcgtcg cgtcggggcg cgttgcggcc
      841 gatcggtcgg ggcgtccgat actggacagg ctggatctgg aaaccttgac cgccaggctt
      901 gcggcgattc ttctgcagga caccgcggac ccttcccgga tcgattcgtc gtttgtcagt
      961 tactgcatgg ctgggggcgg cccgggcgac cccgggtttt ccatacgcct gtggcaactt
     1021 gccgggcggc tggcccacct ttttcgcgag tacgaattca atcgttccgg gatggtcagg
     1081 gaatggctgg cgggaaaaat cgatccttcg gccggggagc ttcacgcaag acaggccgga
     1141 ctttaccgcc aggcttgcag tctgctgtat tacgactggg acgcccacgc ggcccgtccg
     1201 gcacggtcgc tgttccagct tggcgaggaa gtgttccagg atccgggcca ccggcatggt
     1261 atcaggaacg atgaaccgga ttggggcgaa accgcagggg aagttgcgcc ccccgcggga
     1321 acgcttcccg gaaccgtttt catattcggt ctttcgacgc tttcgcgtta tcactgccgc
     1381 cggctgtggg aaatttccag gcggtgcgac atcaggcttt atcacgtcaa cggctgtcac
     1441 gagttctggc aggacgtttc gacgccgttc gaggaacgct ggcacaggat ccgagccgcg
     1501 cgtgtcgttt cggtgccgga tggccagggc caggaactgg cactcgacaa cgacctggac
     1561 gacaaccttc tgttgaaggc atggggaaag gccggtcgcg agacggtacg ccttctttcg
     1621 gaactggagg atcagccctc cggcgtgacg accgaatggg ttgacgaacc ggaaatcgat
     1681 tttgaccgcg cgacggtgct tgatcgcgta cggcacggca tccggacaag gtcgtcgcag
     1741 gtcggcagga cggtcgccga cgacagcctt gtcgtcttga agtgtcccgg caggctgcgc
     1801 gaggtcgagg ccgtcgccga acggatcacc gccttgctgc aggccgatcc tgacctgaaa
     1861 ctttccgaca tcgtcgtgct tgtaaccgac atggcctcct acaagccgct gatatcccag
     1921 gtttttgatc gcgatcgaag ccgtcctgac aacgtctggg atcccattcc gtacaacctt
     1981 gcggactcga ccgccggcac cgacagcgtc tatgcggcgg cggtcgaggc gatgctgaac
     2041 ctggccggcg gcgatttttc aaggggtgcc gtattcaggc ttcttttcaa tccgtgcttc
     2101 atggcggcgg ccggtgtggg gccggcaacc gtcgagggct gggccgggct ggccgacagg
     2161 ctggggatct
//
LOCUS       DAOPPA010000512         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_55031_length_2162_cov_3.080683, whole genome shotgun sequence.
ACCESSION   DAOPPA010000512 DAOPPA010000000
VERSION     DAOPPA010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55031_length_2162_cov_3.080683"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PKH54_12620"
     CDS             <1..1514
                     /locus_tag="PKH54_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00783.1"
                     /translation="AAGLCIGPVLDQDRWAACDDDCCDPCEAEGGCPADCGPGPEFSG
                     TKEYDNATTISIWVHGWSNTNPDEEQYGRERGCGGILQDAANFGVVRPCSREDGTELA
                     PNQIAKFEYFGNTPASWLTPEQIAEIEQYPLGGPKTLHRYALITAMYIRHKLDQTGAT
                     HVNLACHSYGCLISRYMMENNLLGLAAENRFVRWFTSAGVLAGARLARLYDNTSVREV
                     ADLFGISQGDFIVMNPDFVMDNAAAWNHRLYRADSPYLGDMIIHHTCGDDPRIKQALN
                     IALLDLNNPGDEPNDGIMYTLDTFFHDQDEPNRFHSVDGTGLLPSHSYVYVDHMAVPE
                     AEGSVALCAAALFHGRKVEIVLKDILLKDDLESDWGLDKAPAEIVVEHQVRYSPYLPD
                     TFGRNPLISENLIDYRTPEMFQATQGQSVAANQVIFAGPVFDDQQDLWLKAEILEADW
                     YPRFDVRELALGIGDKHDALITFQGQVELRDHEFVAENEKARITIAVRVFSMY"
     gene            complement(1527..>2162)
                     /locus_tag="PKH54_12625"
     CDS             complement(1527..>2162)
                     /locus_tag="PKH54_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00784.1"
                     /translation="PDVNMAGSTTRILMVNGSSDEFFPLTAHMVTYNSIPGTDKRTAI
                     IGNGDHGCCLLIDKLCGLEDTETIEARAELAAKGNQKAWFHRWFGTDPDFSYYPNAPE
                     AQVNPVGGAMYVLAAVDGGGSKMDVESVRFWLSNDLAMNFFSQEMKFNDTLKMWDATI
                     PVAAGPNDIWYVEVVYTDGALVFPDKFLLTSVPRLPGGLVPAIHPINSCVP"
BASE COUNT          466 a          636 c          658 g          402 t
ORIGIN      
        1 gcgcggccgg actgtgcatc ggaccggtgc ttgatcagga tcggtgggcg gcctgcgacg
       61 acgactgctg cgatccgtgc gaggcggaag gtggatgccc ggccgattgc ggacccgggc
      121 cggaattttc gggaacgaag gaatacgaca acgcgacgac catttcgatc tgggtacacg
      181 gctggtcaaa cacgaatccc gacgaggaac agtacggccg cgaacgcggg tgcggcggca
      241 tactgcagga tgcggcgaac ttcggagtgg tgcgcccatg ctccagggaa gacggaaccg
      301 aactggcgcc gaaccagatt gccaagttcg agtactttgg gaacaccccg gcatcctggc
      361 tgacgcccga acagattgcc gaaatcgaac agtacccgct tggcggacca aagacgctgc
      421 accggtacgc cctgataaca gccatgtaca tccgccacaa actggatcag acgggggcga
      481 cgcacgtcaa cctggcctgc cattcatacg ggtgcctgat ttcccggtac atgatggaaa
      541 acaacctgct gggacttgcc gcggaaaacc gtttcgtccg gtggttcaca agcgccggag
      601 tactggccgg ggcacgcctg gcaaggcttt acgacaacac cagcgtccgc gaggtggccg
      661 atcttttcgg aatcagccag ggcgatttca tcgtaatgaa cccggacttc gtgatggaca
      721 acgccgccgc atggaatcac aggctgtatc gtgcggacag cccgtacctg ggcgacatga
      781 taattcacca cacctgcggc gatgacccca ggatcaaaca ggcgctgaac atcgcgctgc
      841 ttgatctgaa caacccgggc gacgaaccga acgacgggat catgtacact ctggacacct
      901 ttttccacga tcaggacgaa ccgaaccgtt tccactcagt ggatggaacg ggcctgctgc
      961 catcgcattc gtacgtttat gtcgatcaca tggcggtgcc ggaggccgaa ggctcggtcg
     1021 cgctttgcgc cgcggcgctt tttcacggcc gcaaggtcga aatcgtgctg aaggacatac
     1081 ttctgaagga cgatcttgaa agcgactggg gactggacaa ggcgccggcc gagatcgtcg
     1141 ttgaacacca ggtcaggtat tccccatatc tacccgacac tttcggtcgg aacccgctta
     1201 tcagcgaaaa cctgattgat taccggactc cggagatgtt ccaggcgacc caggggcaat
     1261 cggtggcagc caaccaggtc atctttgccg gcccggtgtt cgacgaccag caggatttgt
     1321 ggctgaaggc cgaaatcctg gaggccgact ggtatcccag gttcgatgtc cgtgaactgg
     1381 cacttggaat cggcgacaag catgacgcgc tgataacgtt ccagggccag gttgaacttc
     1441 gggatcacga attcgtcgcc gaaaacgaaa aggcccggat cacgatcgcc gtccgcgtct
     1501 tctcgatgta ctgacgccga ccgcgactac ggcacgcaac tgttgatggg atgaatcgcc
     1561 ggcacaaggc cgccaggcag gcggggcacg gacgtcagca ggaacttgtc cgggaaaacc
     1621 agggcgccgt cggtatagac gacttcgacg taccagatgt cgttggggcc ggcggcgacc
     1681 ggaatcgtcg catcccacat cttcagtgtg tcgttgaact tcatttcctg ggaaaagaag
     1741 ttcatggcaa ggtcgttcga cagccagaac cgcaccgact cgacatccat ctttgaaccg
     1801 ccgccgtcaa ccgcggcaag gacatacatc gcgccgccca cgggattgac ctgggcctcg
     1861 ggcgcgttcg ggtaatacga aaagtcggga tcggtgccga accacctgtg gaaccaggcc
     1921 ttctggttcc ccttggccgc cagttcagcc ctggcctcga tggtctcggt gtcttccagc
     1981 ccgcaaagct tatcgatcag caggcagcag ccgtgatcac cgtttccgat gattgccgtt
     2041 cgcttgtcgg ttccggggat cgagttgtac gtgaccatgt gggcggtcaa cgggaagaac
     2101 tcgtcgcttg aaccgttgac catcagtatc ctggtcgtgc tgccggccat gttgacgtcg
     2161 gg
//
LOCUS       DAOPPA010000513         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_55687_length_2144_cov_2.930589, whole genome shotgun sequence.
ACCESSION   DAOPPA010000513 DAOPPA010000000
VERSION     DAOPPA010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55687_length_2144_cov_2.930589"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1267)
                     /locus_tag="PKH54_12630"
     CDS             complement(17..1267)
                     /locus_tag="PKH54_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOD00785.1"
                     /translation="MEGTAITAVSDVRTTRLVGMYDMVLMSAVLALVGIGLLLVFSTT
                     ISPGIDMTDERRIAPFLHQAAAVGIGLLVMVAARMLAPEVFGKAAGPALILSFGLIMA
                     MKLVPGFATTINGSPRWLTIFGVSIQPTEFIKVIWAVYAARFVARHESDINKWFWILI
                     TGLAFCLLVAGPIALLPDVGSALIIALTLGMMLVAGGLNWKNIGILTLPLLVALGVIL
                     KIDPVKFNRFQAHAFLDVTREGVGYQLYQAVLSIGSGGLIGSGLGHGTFHIMRHLPES
                     ESDFIMAVAAEELGFFGLVFIVLLYAIIAVRGFMIARKCQDGFRRNLAFVITALISLS
                     AVIHLCVNVALIPAKGLVCPFMSYGGTAMVVALGCVGILQRLHIDSTARKVVAEEFLK
                     SYEPDADDELDADAGTHASGGREE"
     gene            complement(1270..>2144)
                     /gene="mraY"
                     /locus_tag="PKH54_12635"
     CDS             complement(1270..>2144)
                     /gene="mraY"
                     /locus_tag="PKH54_12635"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD00786.1"
                     /translation="AGCVLVSCLLWCNLRNPLVLITLISGAVFSMVGFIDDWMKVRDH
                     DTKGLSGKLRLAVEFAVVLSLVGWFFVEKGAGTGYDLRLYIPFMSAEKYWIEMSIWAY
                     MILASLLVVGTANATNLTDGMDGLAAGPILSAAGVLLVMAYLTGAQLGKFDVSRYLLI
                     PKVVGATELSVVCASVVGATIGFLFYNSHPAQIFMGDTGALGLGAILGSVALLTKNEL
                     ISILIFGIFFLEAMSVILQTTSFRLTGKRIFPIAPIHHSFKRMGWPEEKIVVRFWIVS
                     LLLALIALGSIKVR"
BASE COUNT          501 a          653 c          615 g          375 t
ORIGIN      
        1 gtcctgctct gattcttcat tcttcacgcc ccccggaagc atgcgtgccg gcatcggcat
       61 cgagttcatc gtcggcgtcc ggctcatagg acttcaggaa ctcctcggcg accaccttcc
      121 tggccgtcga atcgatatgg agacgctgca ggattccgac gcaccccagg gcgacgacca
      181 ttgccgtccc gccatagctc atgaatggac agaccagtcc ctttgccggt atcaacgcga
      241 cgttgacgca caggtgaatc acggccgaca gggaaatgag cgcggtgatg acgaacgcaa
      301 ggttcctgcg gaacccgtcc tggcacttcc gggcgatcat gaagccgcgc actgcgatta
      361 tcgcgtacag caggacaatg aatacaagcc cgaaaaaccc cagttcctcg gccgcgaccg
      421 ccatgatgaa gtcgctttcg gattcgggaa gatgccgcat tatgtggaag gttccgtgac
      481 ccagccccga cccgataagt ccccccgatc cgattgaaag taccgcctgg tacaactggt
      541 atccgacgcc ttcacgagtg acgtccagga acgcatgggc ctggaagcgg ttgaatttga
      601 ccgggtctat cttcaggata acgccaaggg caaccagcag cggcaaagtc aatatgccga
      661 tattcttcca gttcaggccc ccggcaacga gcatcattcc aagcgtcagg gcgatgatca
      721 gcgcgcttcc gacatcgggc agcagggcaa tcgggccggc aaccagcagg cagaatgcaa
      781 gacccgtgat aaggatccag aaccacttgt tgatatcgga ctcatgcctg gccacgaatc
      841 gcgcggcata cacagcccag atgaccttga tgaattcggt cggctggatg gacaccccga
      901 atatcgtgag ccagcgtggt gaaccgttga tcgtggtcgc gaatccggga accagcttca
      961 tcgccatgat cagcccgaac gacagtatca gggccgggcc ggcggccttt ccaaatacct
     1021 cgggggcaag catcctggcc gccaccatca ccagcaggcc gatgccgacg gccgcggcct
     1081 ggtgaaggaa cggggcgatg cgccgctcgt cggtcatgtc gatgccgggc gatatggttg
     1141 ttgagaagac cagcagcagg ccaatcccga caagggccag aaccgcggac atgagtacca
     1201 tgtcgtacat gcccaccagc cttgtggtcc gcacgtcgga gactgccgtg atcgccgtac
     1261 cttccatgat cacctgacct taatcgaacc aagggcaatg agtgccagca acaggctgac
     1321 aatccagaac ctgaccacaa tcttttcctc gggccagccc atccgcttga acgaatggtg
     1381 aatcggcgcg attggaaaga ttcgctttcc ggtaagccgg aatgaggtgg tttgaagaat
     1441 cacgctcatc gcttccagga agaagattcc gaaaatcaga atcgatatca gttcattctt
     1501 ggtcaggagc gcgaccgagc cgagaatcgc accaaggccg agggcgccgg tgtcgcccat
     1561 gaatatctgg gccggatggc tgttgtagaa caggaagcca atcgtcgcgc ccacgactga
     1621 cgcgcagacg accgacaatt cggtggcccc gacgaccttc ggaatcaaca gatacctgga
     1681 cacgtcgaac ttgccgagct gggcaccggt caggtaggcc atgaccagca gaaccccggc
     1741 ggcggataga atcgggccgg cggccaggcc gtccatgccg tcggtcaggt tggtcgcgtt
     1801 tgcggttcca acgaccagca atgacgccag aatcatgtac gcccagatgg acatctcgat
     1861 ccagtacttc tcggcgctca tgaacggtat gtacagcctc aggtcgtatc cggtgccggc
     1921 gcccttttcc acgaagaacc agcctacaag cgacaggacg acggcaaact cgaccgcaag
     1981 cctgagcttt ccggacagac ccttggtgtc gtgatccctg accttcatcc agtcgtcgat
     2041 gaaaccgacc atggagaaga cggcgccgga aatcagcgta atgagcacaa gcgggttgcg
     2101 aaggttgcac cacagcaggc acgagacgag cacgcacccg gcga
//
LOCUS       DAOPPA010000514         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56108_length_2132_cov_3.695715, whole genome shotgun sequence.
ACCESSION   DAOPPA010000514 DAOPPA010000000
VERSION     DAOPPA010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56108_length_2132_cov_3.695715"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..890)
                     /locus_tag="PKH54_12640"
     CDS             complement(6..890)
                     /locus_tag="PKH54_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00787.1"
                     /translation="MVRIFYRLIALAWIAALVPSWAGCLHTPDEDLPFCLHVEIQAQP
                     GQSIGAYSITADDVESGTGAIWCPGADIDPPSEDCARPTILKNLSAGSVLTVRAPGFR
                     NESIILSPAEMPTTDDGCRKLDVTLRAAPEIDRTEDYVTGFTADDGIDSFVELGVLTP
                     TGYGTLVAVKFFIDDINGSPVVYFQNTRKHPLHFNFVRGVLGKSLTSDEYEASTYHGL
                     ERTAMAGNIVWYRDFSVNSRHAGAFSSPMTVEFFPSDDLTPAQALKAYDLIHERIRFL
                     APGGQDNRLAWPHPGRLT"
     gene            complement(900..1631)
                     /locus_tag="PKH54_12645"
     CDS             complement(900..1631)
                     /locus_tag="PKH54_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00788.1"
                     /translation="MKRLPMFVAPLVFSTILLAGCGDSGTDSDVSQDIPADVPADVPD
                     AAQDFTIRKPAQEDLFCQVEFGGEYWLGQLDHVCLIDNSAMKAELYVQADPIDCGQFG
                     NPSYATPAAWLKINGRIHAVAASYDFGGRHNNDLISFTFDSTTYILYHSSIGYGWRAC
                     TKPDCMVVCDPGKECVPDTDVDIAIDGCARAAGDPPPSLPVICVTVEADGSIPEFSDP
                     WTTQDPYGQEGVMILPCPGEESVLI"
     gene            1868..>2132
                     /locus_tag="PKH54_12650"
     CDS             1868..>2132
                     /locus_tag="PKH54_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00789.1"
                     /translation="MWRSGNARLQGLGLAIMVGAVAVFPSTMAPAGAHLPVLRLLPGR
                     HECGDGLLGYPQEACDDANALVGDGCSETCTVESGWRCGNGSDA"
BASE COUNT          428 a          610 c          644 g          450 t
ORIGIN      
        1 aacgatcacg tgagcctccc cggatgcggc cacgccaggc ggttgtcctg gccgccgggg
       61 gcaaggaacc ggattcgttc atggataagg tcgtatgcct tcagtgcctg ggccggcgtc
      121 aggtcgtcac tgggaaagaa ctcgaccgtc attggcgacg agaaggcccc ggcgtgccgg
      181 ctgtttacgg aaaagtcgcg ataccagacg atgttgccgg ccatcgccgt ccgttccagg
      241 ccgtggtacg tcgaggcctc gtattcgtcg ctggttaacg acttcccaag aacgccccga
      301 acgaaattga aatgcagcgg atgcttcctg gtgttctgga aatagacgac cggactgccg
      361 ttgatgtcgt cgatgaagaa cttgaccgcc acaagggttc cgtaaccggt cggggtcagg
      421 acgccaagtt cgacgaatga gtcgattcca tcgtccgcgg tgaatccggt cacatagtct
      481 tcggttctat ctatttcggg cgccgcccgc agcgtgacgt caagttttct gcagccgtcg
      541 tcggtcgtgg gcatttcggc tggtgacagg atgatgcttt cgttgcgaaa tccaggtgcc
      601 ctgacggtca gaactgaacc ggctgacagg ttcttcagaa tcgtcggccg ggcacagtct
      661 tccgacgggg gatcgatgtc ggcgcccggg caccagatcg cgcccgtccc ggattcgaca
      721 tcgtcggcgg taattgaata cgcgccgatt gactgccccg gctgggcctg aatttccacg
      781 tgcaggcaga acggaaggtc ttcatccggg gtgtgcaggc atccggccca ggatggaaca
      841 agggccgcga tccacgccag ggcgatcaat cgataaaaaa tcctgaccat gatttcgccc
      901 tatatcagaa ccgattcctc gccgggacag gggagtatca tcaccccttc ctggccgtag
      961 ggatcctggg ttgtccatgg atcgctgaat tccggaatgc tgccgtccgc ctcgaccgtc
     1021 acgcatatga ccggaagcga aggcggggga tcaccggccg cgcgcgcgca gccgtcgatt
     1081 gcgatatcga cgtcggtatc cggaacgcac tcctttccgg gatcgcatac caccatgcag
     1141 tccggcttcg tgcaggcacg ccagccgtaa ccaatcgacg agtggtacag aatgtaagtc
     1201 gttgaatcaa aggtaaatga aatcaggtca ttgttatgcc ttccgccgaa atcgtaactg
     1261 gccgccaccg catgaatccg tccgttgatt ttcagccagg ccgccggggt tgcgtatgag
     1321 ggattgccga actggccgca gtcgatcggg tcggcctgga cataaagttc ggccttcatc
     1381 gctgaattgt cgatcaggca gacatgatcc agttgcccga gccagtattc gccaccaaat
     1441 tcgacctggc agaagagatc ctcctgagcc ggcttcctga tggtgaaatc ctgtgcagca
     1501 tccggcacat cggcgggcac atcggcggga atgtcctgtg aaacatccga atccgttccg
     1561 gaatcgccgc agccagccag caggatcgtt gaaaaaacca gtggcgccac aaacattgga
     1621 agtcttttca ttttgattct tccccggggt gtttcccctg acaagggccg cttcgggcct
     1681 gccgaaagga ttctgcgatt atttttaagg aatgtccaat ggttgaagcc cccttgccca
     1741 cccatgccgc ctgcggaagc gtattatggc gatgacgaca ttggatataa ctgaatgcgg
     1801 cagaaacatt gtattattgg cctgcattcg aaccttaccg gcgatgtggc ggcgggggcc
     1861 tggattcatg tggcgaagcg gaaatgcacg gttgcaggga ctcggtctcg ccatcatggt
     1921 cggcgccgtt gccgtctttc catcgacaat ggctccggcc ggcgcccatc ttcctgttct
     1981 gcgactgctt cctggcaggc acgagtgcgg cgacggcctt cttggatatc cgcaggaagc
     2041 ctgtgatgac gccaatgcac tggttggcga cggatgctcg gaaacctgca cggtcgagtc
     2101 cgggtggcgt tgcggcaacg gttcggacgc gg
//
LOCUS       DAOPPA010000515         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56116_length_2132_cov_3.354839, whole genome shotgun sequence.
ACCESSION   DAOPPA010000515 DAOPPA010000000
VERSION     DAOPPA010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56116_length_2132_cov_3.354839"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKH54_12655"
     CDS             <1..403
                     /locus_tag="PKH54_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00790.1"
                     /translation="EPFCDVTESGALYMAGNVWEWVQDFLHDSYADVFSNGQYEDAPA
                     DGSEWLFDLGGRRVARGGRPDAVNPTDHRLHRRYAEFPPFQDDSLGFRCCANFVCDTD
                     DDCAALSSARPYCLTRPDGVNYCSATPAESD"
     gene            645..1934
                     /locus_tag="PKH54_12660"
     CDS             645..1934
                     /locus_tag="PKH54_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD00791.1"
                     /translation="MTRRARVVLFPAVILATIIVCGFQFPAAAQTGQRSQDLYTLIEQ
                     AQAHVVAGRWQSAITVYLDAWKVSRDPGYLYNIAVLYLVRLKDEAKALDYAEKYSEQA
                     RTDSEKLEAEGLIRRIRDSIKGTHGRIVVQVLPARAQAVVYVDNRPLGEDNWVTAGVH
                     TLGVQAPGYVPFSREIEVVAGTETSFSVQLKTVEGQVSVRCDGGPCKVLVDGDEIGQA
                     PVSRALSPGEHSVEIDASGRQLFSDWVVIESGMKSSLVVSVDSGRVEVTTESIGAEPG
                     IAVVEDEKPATESKADDAGKQWAKQVDTQSARTSKIGPQKAAAISMWVLSGVVLGVGT
                     GMYFLAQDKLDTAALYYETFLRTTKENERLWLYREQKKLIGMADTLSYVAYGSWGLSG
                     AALITGLALWFTAPADSAVSVLPSGPGGPGATVLVRW"
BASE COUNT          383 a          614 c          654 g          481 t
ORIGIN      
        1 cgagccgttc tgcgatgtca ccgaatccgg tgccctgtat atggccggca acgtctggga
       61 atgggttcag gattttctgc acgactcgta cgccgacgtg ttttccaacg ggcagtacga
      121 agatgccccg gccgacggat ccgaatggct gttcgatctt ggcgggcgcc gcgttgcgcg
      181 tggcggacgc cccgatgcag tcaatccgac cgaccacagg cttcatcgtc ggtatgccga
      241 attcccgccg ttccaggacg actcgctcgg tttccgctgc tgcgccaact tcgtctgcga
      301 caccgacgat gactgcgccg ccctttcgtc ggccaggcca tactgcctga ccaggcctga
      361 tggcgtgaat tactgctccg ccaccccggc cgaatcggac tagctggtca taagtctttt
      421 ctgcaatatc cgatttttcc aaccggtcga agctcgcccg ccaataccgg aaatcgccct
      481 acagccttat ttaattggcg gtttcggcac aatgtgcgtt agaatagcgg ttagtttatt
      541 ctgtccggcg tccacgcacg gcccgggctc gcgctggaag atacgttcgt gccggcggac
      601 gcccctgatt tccttcgtgg aattcaggtc ttggagtatt gctgatgact cgtcgagctc
      661 gagtggttct ctttcccgcc gtcattcttg cgacaattat cgtctgtggc tttcagtttc
      721 cggcggccgc ccagaccggg cagaggtcgc aggatcttta taccttgatc gagcaggccc
      781 aggcccacgt cgttgcagga cgctggcagt ccgcgattac cgtctatctg gacgcatgga
      841 aggtttcccg ggatcccggt tatctgtaca acatcgctgt tctttatctg gtcaggctga
      901 aggacgaggc caaggcgctt gattacgccg agaagtattc cgagcaggcc aggaccgatt
      961 ccgagaagct cgaggccgag gggctgatca ggaggattcg cgacagcatc aagggaaccc
     1021 acgggcggat cgttgttcag gttctgcccg cccgggcgca ggccgttgtc tatgtcgata
     1081 acaggccgct tggagaggat aactgggtca ccgccggcgt tcacacgctt ggcgtccagg
     1141 cgccgggata tgtgccgttt tcgcgtgaaa tcgaggttgt cgcaggcacg gaaacctcat
     1201 tttcggttca gttgaagacc gtcgagggcc aggtctcggt ccggtgtgac ggcggtccct
     1261 gcaaggtgtt ggtggatggc gacgaaatcg ggcaggctcc tgtttcacgc gccctcagtc
     1321 cgggcgagca ttccgtggaa atagacgctt ccggccgcca gctcttttcg gactgggtcg
     1381 tcattgagtc aggaatgaaa agctcgctgg tcgtgtcggt tgacagcggc cgcgtcgaag
     1441 tcacgaccga atcgattggt gccgagccgg gaattgccgt cgtcgaggac gagaagcccg
     1501 ccaccgagtc gaaggccgac gatgccggca agcagtgggc aaagcaggtt gacacccagt
     1561 cagcaaggac ttcgaaaatt ggtccccaga aggccgcggc catttcgatg tgggtattgt
     1621 ccggagttgt gctcggcgtc ggaaccggca tgtacttcct tgcccaggac aagcttgata
     1681 ccgctgccct gtattacgag accttcctgc gcacgaccaa ggaaaacgag cgcttgtggc
     1741 tgtaccgcga acagaagaag ttgattggga tggccgacac gctttcctat gtcgcctatg
     1801 gatcctgggg cttgagcggt gccgcgctga tcaccgggct tgcgctgtgg ttcacggcac
     1861 cggccgactc cgcggtttcg gtccttccgt ccggccccgg cggtcccggt gccacggtgc
     1921 tcgttcgctg gtaagtggtg tgtcacacct gataactgga cggttcgcct tatgaaaaag
     1981 cgttttatcc tgcttgcagt cgcgatggtt cttccgctgg tcactgcatg tcttcagtat
     2041 cccgacagga tcccgaatcc cgacagcatt gtcgatgccg tggatgacgt ggtttcggac
     2101 gtcaattctg aagatgtgac ggatgccgtg ga
//
LOCUS       DAOPPA010000516         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56427_length_2123_cov_4.437137, whole genome shotgun sequence.
ACCESSION   DAOPPA010000516 DAOPPA010000000
VERSION     DAOPPA010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56427_length_2123_cov_4.437137"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..585
                     /locus_tag="PKH54_12665"
     CDS             157..585
                     /locus_tag="PKH54_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00792.1"
                     /translation="MEPIQSVDFRDGPAESEGVRRLVADMRDRVFVRVRDSIDAGDGC
                     FAIRVPRFDGTILGRFLMPRLRRPCFNIRLDRSGSFVWERIDGLNAVGRIAAEWAVAF
                     PGERLPDARVTLFIRALAVQGHIREVDPEGAACVTSDSGR"
     gene            613..1788
                     /locus_tag="PKH54_12670"
     CDS             613..1788
                     /locus_tag="PKH54_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD00793.1"
                     /translation="MDIRAIQKAIGEAGFDGWLFCDFNNRDALAYRILGLDFQKYTSR
                     RWFYLVPASGEPVRLVSKVEPSRLDTLPGEKRTYLSWRDLSEELERMLSGRLVAMQYS
                     PEDSIPYISIVDAGMVELVKAAGARIASSADLVQVFEAVIGEEGYRSHVEAGRIIHAI
                     KDQAFALIGEHVRSGRDITEYEVQRFILRRFDEEGLDCMGEVPIVGVNGHPSDPHFEP
                     TPENTVVIREGDTVLIDLWAKFAKPGSIFYDITWCGFVGTNPPDEYVKIFETVRDARK
                     AAVSFVRERFARHQKCFGYEVDDACRSVVQASGWGDCFIHRTGHSIGVNVHGNGVNID
                     NLETRDLRMLVPGICFSVEPGIYLAGRMAVRSEIDVFIRKDGSVEVTGPEQETLILV"
BASE COUNT          412 a          567 c          671 g          473 t
ORIGIN      
        1 gttttgccga gaacgagaag tggcttccgg ggctgggatg gattgaatac agcggcctgt
       61 cccgccattg tttcaagatc agtccggatg acctgcgcgc atttatcagg gacgttgcgg
      121 gccttgattg agcgtcgcga cgccggaagg aatgcaatgg aaccaattca atcagtcgat
      181 ttccgcgatg ggccggccga gtccgaaggt gtgcggcggc tggtggccga catgcgggat
      241 cgcgtgtttg tgcgggttcg cgattcaatc gatgccggtg acggctgttt cgcgatccga
      301 gtccccaggt tcgatggaac catccttgga cgattcctga tgcctagact gcgcaggccg
      361 tgcttcaaca tccggctgga tcgcagcgga tcgttcgttt gggagcgtat tgacggtttg
      421 aacgcggttg gacgtatcgc cgccgaatgg gctgtcgcct ttcccggcga gcgcctgccc
      481 gatgcccgcg tcaccctttt catcagggcg cttgcagtcc agggacacat cagggaagtc
      541 gatccggaag gtgcagcttg tgtgacgtct gactcggggc gataatgcca ttccttcagc
      601 agggggttac ccatggacat cagggcgatt caaaaggcga tcggggaggc cggtttcgac
      661 ggctggctgt tctgcgattt caataacagg gatgcgctgg cgtacaggat actgggcctg
      721 gatttccaga agtacacgtc ccggcgctgg ttttacctgg tgccggcttc cggggagccg
      781 gtacggctgg taagcaaggt ggagccttca aggcttgaca cgctgcccgg cgagaagcgg
      841 acgtatctgt cgtggcgcga tctgtccgag gaactggaac gcatgctttc aggcaggctg
      901 gttgcgatgc agtactcacc cgaggactcg attccgtaca tcagtatcgt cgatgccggc
      961 atggtggaat tggtgaaggc cgccggggcg aggatcgcgt cgtccgccga tctggtccag
     1021 gttttcgaag cggtcatcgg cgaggaaggt taccgcagtc atgtcgaggc cggacgcata
     1081 atccacgcga tcaaggatca ggcgttcgcg ctgattggcg aacacgtcag atccgggcgc
     1141 gacatcaccg aatacgaagt ccagcgtttc atcctgcggc gttttgacga ggaaggcctg
     1201 gactgcatgg gcgaggttcc aatcgtcggt gtcaatgggc atccttccga tccgcacttc
     1261 gagccgaccc cggaaaacac cgtggtgatt cgcgagggcg acaccgtcct tattgacctg
     1321 tgggccaagt tcgcaaagcc gggatcgatc ttttacgaca tcacgtggtg cgggttcgtg
     1381 ggaacaaatc cgccagatga atacgtgaag attttcgaaa ccgtccgcga tgcccgcaag
     1441 gccgccgttt cattcgtgcg ggaacgtttt gccaggcacc agaagtgttt cgggtacgaa
     1501 gttgatgacg catgccgttc ggtggtccag gcatcgggat ggggcgactg cttcatccat
     1561 cggacaggcc attcgatcgg cgtgaacgtg cacggtaacg gcgtcaacat cgacaacctt
     1621 gaaacccgtg atttgcgaat gctggttccc ggaatatgtt tttcggtcga accgggcata
     1681 tatctggccg ggcggatggc cgtcaggtct gaaatcgacg tgttcatcag gaaggatgga
     1741 agcgtcgagg tgacgggtcc cgaacaggaa acgttgattc ttgtctagcc cggagttccg
     1801 tagcgatttg agcgttatct tcccgtaata cttcagttat acgccaaatg cagtgttgga
     1861 tttctgacta cagccgcgcc gtgccgtcga gccttgtagt gaccgatctg cgccagcgga
     1921 caagcgtggt tctcgtacac gctgttgcgc ttgcggcctc gctcaaacaa aattttcagg
     1981 cgaaatactc cgggctagcg ggcgcgattc cttccgaacc tgatcctcca cggaatgccc
     2041 aggctttccc agaaaatccc gccgaccagc tttgattcgc ccttggtcct gtccggaaag
     2101 atgatcggga tttcggcgac ctt
//
LOCUS       DAOPPA010000517         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56510_length_2121_cov_4.109390, whole genome shotgun sequence.
ACCESSION   DAOPPA010000517 DAOPPA010000000
VERSION     DAOPPA010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56510_length_2121_cov_4.109390"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1996)
                     /gene="fusA"
                     /locus_tag="PKH54_12675"
                     /pseudo
     CDS             complement(1..1996)
                     /gene="fusA"
                     /locus_tag="PKH54_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682368.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(2018..>2121)
                     /locus_tag="PKH54_12680"
     CDS             complement(2018..>2121)
                     /locus_tag="PKH54_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD00794.1"
                     /translation="IIDAFNGRGNSIKKRDDTHRMAEANKAFAHYRW"
BASE COUNT          429 a          674 c          612 g          406 t
ORIGIN      
        1 aaagggaacg caaatccgtg acatagccga acatttcccc gagcggaacc tcggcgccga
       61 cgacatgcag tcaatccgac gtatccaccc tcacaaccct ggcccgcctg cttgacaggt
      121 tgccaaggac atctcccagg aactcggccg gcaccgtcac ttccagggcc attaccggct
      181 ccaggatgac aggcttgccg cgcgcgacca gatccttgat cgccatcgaa ccggcgattc
      241 ggaacgccat ctccgaagaa tcgacttcgt gatagcttcc gaagctgaca cggaccttga
      301 agtcgattac cggataaccg gccaggatgc cggaggcagc cgactcgcga acaccgcgct
      361 cgattgccgg catgtactcc ctcggtatca cgccgccgac caccgcgttc tcaaaaacca
      421 tcccggcgcc gcgctcaagg ggttcgatat ccatcacgac atgggcgtac tgcccgtggc
      481 cgccggtctg cttgacgtac ctgacctcgg cctcggccgg acgggtgacc gtctcgcgat
      541 atgcgacctg gggctgtccg acccttgcct tgacgccgaa ctcgcgacgc aggcgatcga
      601 cgatgatatc gaggtgaagc tcgcccatcc cggaaatgat ggtctgggcg gtctcgctgt
      661 cgacacggaa cttgaacgtc ggatcctcga gggacagccg acggagggat tcaaagagct
      721 tgtcggaatc ggttgacgac tcgggctcga gggccacgtt cacgaccggc tccggaaaat
      781 ccattgtttc aagaacgacc ggagcatttt ccgagcagag cgtgtcaccg gtcaccgttg
      841 acttcaaacc gacgacgccg ccaatctcgc cagccttcag ttccgcgact tcctcgcgct
      901 tgttggcgtg gatgcggacc aggcgattga ttcgctcgcg cttctgagta ttcgcgttat
      961 agacggtttc accggccttg accgagcccg aatagacgcg aagaaacgtc agctgaccga
     1021 cgaacgggtc gttcatcagc ttgaacgcga gcccgcagaa cggctccgac agggagcacg
     1081 aacgctcgac gaccggacgg tctggcccgg aaacctttcc gtccgcaccc tgaaccggag
     1141 gcagatccgc cggaccgggc agataatcaa cgacaccgtc caggagtggc tgaacgccct
     1201 tgttcctgaa agccgatccg caataaaccg ggatcacttc gctctggatg gtcgcggttc
     1261 gaatggcgga cttgatttcc gtcgtcgaga ggtgttcccc ggaaatgtac ttctcgagga
     1321 tcgcctcgtc gaattcggcg acggcatcga tcagttcgtt ccgtgcttcc tcggcggcgc
     1381 aggccatctc ggtcggaacc ggcatcgtcg tgtaacgcga tccaagaacg tcctgatccc
     1441 agacaagagc ctgcatctcg accagatcga ttacgccgcg gaaaccggat tcggatccaa
     1501 tcggcaactg gaccggaacg gcccgggccc gcagtttgga acgaatctgc ccgaccacgc
     1561 gacggaaatc cgccccggtt cgatccatct tattaatgaa ggcgatcctg gggacgcggt
     1621 acttgtcggc ctggcgccag accgtctcgg actggggctc gacgccgccg accgcgcaga
     1681 acaccacgac gccgccatcg aggacgcgaa gcgacctttc cacctcgacg gtgaaatcga
     1741 cgtgcccggg ggtgtcaatg atgttgatgg aatagtcgtg ccagaagcat gtcgttgcgg
     1801 ccgacgttat cgtaatgccg cgttcctgct cctgctccat ccagtcaagc tgcgtatcgc
     1861 cggaatccac gttaccaagg cggtgcgaag aaccggtgta gtacagcacc cgctcggatg
     1921 tggtcgtctt accggcgtcg atgtgagcca tcacgccgat attgcgaatc cggttgatgt
     1981 tgtacgtttc cgccacttcg gttgccccgg gatactacta ccagcggtag tgcgcgaacg
     2041 ccttgttggc ctccgccatg cggtgcgtat cgtcacgctt cttgatcgag tttccacgac
     2101 cgttgaaggc gtcgatgatt t
//
LOCUS       DAOPPA010000518         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56621_length_2118_cov_3.899661, whole genome shotgun sequence.
ACCESSION   DAOPPA010000518 DAOPPA010000000
VERSION     DAOPPA010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56621_length_2118_cov_3.899661"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKH54_12685"
     CDS             complement(<1..795)
                     /locus_tag="PKH54_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00795.1"
                     /translation="MTNKFGSLAKLTIVAIAFGFAACTGITSQTDDGPADIPVVIPDY
                     GQEVDVIVDPDVNPGDPGATDADRDQGGEVTDQGGLTDNGDVPDDGSSVDVGPGDAVE
                     VTDNVGTDLNETIDDPGVEPDGCVPQCGVMGTETERECGPDQCGDDNGCGFCSTGYVC
                     DRVAGKCILMCIPNCLELGKVCGDDGCNGNCGECGDGYECGLDFKCYQSECKSDCIEK
                     GMECGVDKCGDSCGTCGGSEVCSAGGKCAPGACFGIDKDRNSCSPDS"
     gene            976..>2118
                     /locus_tag="PKH54_12690"
     CDS             976..>2118
                     /locus_tag="PKH54_12690"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD00796.1"
                     /translation="MKMAGFALVTSMMLAVPMFSAVAQETVPLDQDLAWMAVERNSRS
                     TDINARAEAIRAMRFAAGRPTAQVLQDSLADSQWSVRRHAIIGLVLAGDPKAQGLVAE
                     SLRNQSIPLEAEALELVGYLPPASADQVFKAALLEAANPARDLLMNNVLSGCPDVMAK
                     WFAEGLVADPAWFIDKFEKVPKDVLPALLDKLAGDKRPEVFEPAVKVAAMKGLKIDMA
                     VLNAVMKSKDATLRVMAAEAAARQGNQDAIAILLPLADGSEADRIRFLKAAAAAPSPV
                     AQQKAWGMMKPDINPEMVNLIYMCFPATDTRDVLKALDADVESTDAARRPGAVKAVGR
                     VKGNRALPQLYDLMSNAGNPNVRTAAAQAIGDLAQVESIPALHKALN"
BASE COUNT          420 a          662 c          627 g          409 t
ORIGIN      
        1 tgaatccgga ctgcacgaat tcctgtcctt gtcgatcccg aagcacgcgc caggggcaca
       61 cttcccaccg gccgaacata cttcgctgcc gccgcaggtg ccgcaggagt cgccgcattt
      121 gtcaacgccg cactccatgc ccttttcgat gcagtccgac ttgcattcgg actgatagca
      181 cttgaagtca aggccgcact catagccgtc gccacactcg ccgcaatttc cgttgcagcc
      241 gtcgtcgcca cagaccttgc caagttcaag gcagttgggg atgcacatca ggatgcactt
      301 gcccgcgacg cggtcgcaga cataaccggt cgagcagaat ccgcacccgt tgtcgtcgcc
      361 gcattgatcc ggcccgcatt cacgttcggt ttcggttccc atcacgccgc actggggaac
      421 gcatccgtcc ggctcgaccc ccggatcatc gatcgtttca ttcaggtcgg tgccgacgtt
      481 gtcggtcact tcaaccgcgt cgcccggacc cacatcaacg gacgagccgt cgtcgggaac
      541 gtcgccgtta tcggtcaggc cgccctgatc ggttacttcg ccgccctgat ccctgtcggc
      601 atcggttgca ccggggtcac ccggattgac gtccggatcc acgatgacat cgacttcctg
      661 gccgtaatcg ggaataacca ccgggatatc cgccgggccg tcatccgtct gggaggtaat
      721 gcccgtgcag gcggcaaagc cgaatgcgat cgcgacaatc gtcaacttcg cgagactgcc
      781 gaatttattc gtcatgagag accccgaaag ccgtacgact gccattttac agaaaaatgc
      841 aaacttggca gccttcttgt ggcaaaaatt actgaccttt gataaacaga tacatcggaa
      901 ggataatatc cgcgcctccc ggaaaaggga agttgacgat atttgccgga catctgcatt
      961 cggaggaatg gtttcatgaa aatggcgggt tttgcgcttg tgacttcaat gatgctggcc
     1021 gttccgatgt tctcggccgt ggcccaggaa actgttccgc tggatcagga tcttgcctgg
     1081 atggccgttg aacgcaactc cagaagcacc gatataaacg cccgcgccga agcgattcgc
     1141 gcgatgcgtt tcgctgccgg acgtccgacg gcccaggtgc ttcaggactc gctggccgac
     1201 agccagtggt cggtccgcag gcacgccatc atcgggcttg tgcttgcagg tgacccgaag
     1261 gcccagggcc ttgtggccga aagcctgcgg aaccagtcga ttccgcttga agccgaagca
     1321 ctggaacttg tcgggtatct tcccccggcc agtgccgacc aggtgttcaa ggcggccctg
     1381 ctggaagcag ccaacccggc gcgcgacctg ctgatgaaca acgtgctttc cggttgtccg
     1441 gacgtgatgg ccaaatggtt tgccgaaggg ctggtggccg atccggcgtg gttcatcgac
     1501 aagtttgaaa aggttcccaa ggatgtcctt ccggcgctgc tggacaagct ggcgggcgac
     1561 aagcgtcctg aagtattcga gccagcggtg aaggtcgcgg ccatgaaggg cctgaagatt
     1621 gacatggctg tcttgaacgc cgtcatgaag tcaaaggacg ccacgcttcg ggtgatggcc
     1681 gccgaagccg ccgcgcgcca gggcaaccag gacgccatcg ccatccttct gccgcttgcg
     1741 gacggaagcg aggccgaccg gatccgcttc ctgaaggcgg cggcggcggc gccgtccccc
     1801 gttgcccagc agaaggcctg ggggatgatg aagcccgata tcaaccccga gatggtcaac
     1861 cttatttaca tgtgtttccc ggcgaccgac accagggacg ttctgaaggc gcttgacgcc
     1921 gacgttgaaa gcaccgatgc cgcaaggcgg cccggcgccg tcaaggcggt tggacgggtc
     1981 aagggaaacc gtgcccttcc ccagctttac gaccttatgt ccaacgccgg aaatccgaac
     2041 gtgcggactg ccgcggccca ggcgatcggc gatctggccc aggtcgagtc gattccggcc
     2101 ctgcacaagg cgcttaac
//
LOCUS       DAOPPA010000519         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56699_length_2116_cov_3.669093, whole genome shotgun sequence.
ACCESSION   DAOPPA010000519 DAOPPA010000000
VERSION     DAOPPA010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56699_length_2116_cov_3.669093"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="PKH54_12695"
     CDS             complement(<1..1517)
                     /locus_tag="PKH54_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00797.1"
                     /translation="MTITRLVSAAGFFVLAASFGCGGDGSADDTDVVSEEVVSGDSAE
                     GDLVPNPDSLDANLPDALTDAEGHQDARQDISADVEPDVAGDGLDAEDPGLDGSAGDA
                     GDSGTGELPQAIAEIDRALDPAFLQPSLGFTFRIVTPLMAEYPAAGPNPSDLAWIIQS
                     ELVLPSGDVVRLEERGDTFAVLYPIDGYDDSWIFINGGGTDVVSGDIYTLNLYPMLST
                     MQWMKENGIDEISGEYAPFVVYRTGTFRGVWVSCPAMVGDSPSLSKMRAYFGPDTLQP
                     GSPVGLMVNSYVTGDRSVISKSFGVSQYHQACTCYDVVGMTTKERPCTAGDIDQYPGQ
                     AAVEFPPDGAEAVAAVGTALQWGSSGDADGGTVSFDVWLGTDRMTLAKVAGPIQSVSY
                     QPNLVRDTTYFWRVDVRDDEGNLVPGRVWTFNTDTAVPQLNDHNYLILVDRRLKGQLD
                     DELATYVQDVVNQDDTLVPAVRYWMPGDHVMMRDIIIDSFMNRCVAEDDCLRGVFL"
     gene            1739..>2116
                     /locus_tag="PKH54_12700"
     CDS             1739..>2116
                     /locus_tag="PKH54_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00798.1"
                     /translation="MTVTGIRKFAATVLATVALTACQGGDPEVPVGHETVNLSQWAIA
                     SSQGLDADGAAISTPGFDDSGWIPATVPGTVAGSQMDAGLLGNPFFGTALQEVPGWTY
                     SFLPMPEDSPYRHAWWYRTEVTVP"
BASE COUNT          459 a          671 c          595 g          391 t
ORIGIN      
        1 aggaacaccc cgcgcaggca gtcatcttcc gcgacgcacc gattcatgaa gctgtcgatg
       61 atgatgtccc gcatcatgac gtgatcgccg ggcatccagt accgtacggc cggaacaaga
      121 gtgtcgtcct gattcaccac atcctggaca taagtggcca gttcgtcgtc aagctgacct
      181 ttcagcctgc gatcaaccag gatcaggtag ttgtgatcat tcaactgggg aacagcggtg
      241 tcggtgttga atgtccagac cctgcctggc acgaggttgc cttcgtcgtc acggacatcc
      301 acccgccaga agtacgtagt gtcgcgcaca aggttcggct ggtacgaaac cgactggatc
      361 ggaccggcaa ccttcgcgag cgtcatacga tcggttccaa gccacacgtc gaaagaaacg
      421 gtgccgccgt cggcatcgcc ggaagaaccc cactgaagcg ccgtcccgac agcggccacg
      481 gcctccgcgc cgtctggcgg gaattccacg gcggcctgtc ccggatactg atctatgtcg
      541 ccggccgtgc acggcctttc cttggtcgtc attccgacca cgtcgtaaca ggtacaggcc
      601 tggtggtact gggaaacgcc gaacgatttg gagatgaccg accggtcgcc ggtgacgtac
      661 gaattcacca tcaacccgac cggacttccc ggctgaagcg tatccggacc gaaataggcc
      721 ctcatcttag aaagcgaggg actgtcgccg accatcgcgg gacacgaaac ccagaccccc
      781 cggaacgtcc ccgtccgata gacgacgaat ggagcgtatt ccccgctgat ctcgtcgatt
      841 ccgttttcct tcatccattg catggttgaa agcatgggat acaggttcaa cgtatagatg
      901 tcgccgctga caacgtccgt cccgccacca ttgatgaaaa tccacgaatc gtcataaccg
      961 tcaatgggat acagcaccgc gaacgtgtca ccgcgttctt caagacgcac gacatccccg
     1021 gaaggaagaa ccagttccga ctgtatgatc caggccagat cggaaggatt cgggccggcg
     1081 gccggatact cggccatcag gggcgtcacg atcctgaacg tgaatccaag ggacggctgc
     1141 agaaaggcgg gatccagcgc cctgtcgatt tcggcgatcg cctgtggaag ttcaccggtg
     1201 ccggaatcgc cggcgtcacc agccgaaccg tcaagtccgg ggtcttcggc gtccagcccg
     1261 tcgccggcaa catccggttc gacatctgcg gaaatgtcct gccgggcatc ctgatgccct
     1321 tccgcatccg tcaaagcatc cggaagattc gcgtcgagcg aatccggatt cggcacaagg
     1381 tcgccttcgg cggaatcgcc cgaaacgact tcctccgaca cgacgtcggt atcgtcggcc
     1441 gaaccgtcgc caccgcaacc gaaggacgcc gccagaacga agaagccggc cgccgaaaca
     1501 agtcttgtaa tcgtcatgaa ttaaggttcc cgaaagattc cgggcggaat gataccagaa
     1561 tccaccgatg ccctgtgacg ccaatcagga aggcaagtct ttacacaatc acatccccgc
     1621 gaattttcga cagtttctgc cagttcttga ccgaatccgc ggaaaccctt aatctgacgg
     1681 acagttacag ccgggatgac catcctggaa acttatcgtc agcttgaagg ggcctttgat
     1741 gacagtcact ggaattcgaa aattcgccgc gaccgtgctt gcgaccgttg ctttgacagc
     1801 gtgccagggg ggcgacccgg aagtccccgt cggtcacgaa accgtcaatc tttcgcaatg
     1861 ggccatcgcg tcttcccagg gccttgatgc ggacggcgcg gcgatctcga cccccggatt
     1921 cgacgactcc gggtggatcc cggcgaccgt ccccggaact gtcgccggca gccagatgga
     1981 cgccgggctg cttggaaacc cgtttttcgg caccgcgctg caggaagtcc ccggttggac
     2041 gtacagcttc cttccgatgc ccgaggacag cccataccgc cacgcctggt ggtacaggac
     2101 cgaagtcacg gtgccc
//
LOCUS       DAOPPA010000520         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56795_length_2113_cov_3.810982, whole genome shotgun sequence.
ACCESSION   DAOPPA010000520 DAOPPA010000000
VERSION     DAOPPA010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56795_length_2113_cov_3.810982"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKH54_12705"
     CDS             complement(<1..455)
                     /locus_tag="PKH54_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOD00799.1"
                     /translation="MAFSSKCVECVPNFSEGRNREIIAAITGAMERAGATVLDVDMGA
                     AANRTVVTMAGAPETVLEAAFMAIRTAAERIDMSVHKGEHPRMGATDVCPFVPINGVS
                     MAECVELARRLGERVGRELGIPVFLYEEAATIADRRSLADIRAGEYEALP"
     gene            complement(473..913)
                     /locus_tag="PKH54_12710"
     CDS             complement(473..913)
                     /locus_tag="PKH54_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00800.1"
                     /translation="MKPGFKIAVGLAVLGTTILGCVGAPLSASEQALKRLKVTVAQLD
                     GLQPVTIRFNPARCECPAFEAMLDGRWHRIEMSKDDESISASLEADAIRKGPGWTASV
                     LAKGDGIERKLYRSPVIRIAVDAVCQDGECKGLQDGTGSDGDGN"
     gene            complement(918..1805)
                     /locus_tag="PKH54_12715"
     CDS             complement(918..1805)
                     /locus_tag="PKH54_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HOD00801.1"
                     /translation="MISQARQIVATMRPAQWVKNVFVFAPVVFASDHTRKDPSLILYA
                     TAATIVFILLAGAVYIFNDILDLEKDRQHPTKKNRPLAAGKLAVPNAAVGAGLALAAS
                     YLVGYFLGWRFLLAASGYLVLNFAYSTRLKKVVYVDVLCIATGFLLRILAGCFAISLA
                     PAEISYFLIACTFLIASFLALAKRRHEAAVLEGSDTRDVLRDYDVRHLDVVMTIVAVL
                     TIGAYGLYAFSGRTSGYFGNHRMPLTIPFVIIGIVEFIRILRRPGENGSPTDRMLRDP
                     IFIINGVGYAAMVVWVVYG"
     gene            complement(1798..>2113)
                     /locus_tag="PKH54_12720"
     CDS             complement(1798..>2113)
                     /locus_tag="PKH54_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD00802.1"
                     /translation="LKGAGTIIANPDGRLAICDLGTPALAVAGSGDVLSGIAGALLAR
                     GIPPFDAACWAVTVHAIAGRVASRRFGEHGTTASDLIDSIPDAVLAASRGYDGTTGDS
                     DD"
BASE COUNT          434 a          665 c          613 g          401 t
ORIGIN      
        1 ggcagagcct cgtattcgcc ggccctgatg tcggccaggc tgcgccgatc cgcgatggtc
       61 gcggcctcct cataaaggaa aaccggtatg ccaagttcgc gtccgaccct ttcgccgagc
      121 cgcctggcca gttccacgca ttccgccatc gacacgccgt tgatgggtac gaaagggcag
      181 acgtcggtgg cgcccatcct gggatgctcg cccttgtgaa ccgacatgtc tattcgttcg
      241 gccgccgtcc tgattgccat gaaggccgcc tcgagaacgg tttcgggggc gccggccatc
      301 gtgacgacgg ttcggttggc cgcggcaccc atgtcaacat cgagcaccgt tgccccggcg
      361 cgctccatgg cgcctgttat tgcggcgatg atttcccggt tcctgccttc gctgaagttc
      421 ggaacgcact ccacacactt tgatgaaaag gccataaaac ctccgactgc ggttaattac
      481 cgtcgccatc cgaaccggtt ccatcctgaa ggcccttgca ttcaccatcc tgacagacgg
      541 cgtcaacagc gatcctgata accggtgaac ggtaaagctt tcgttcgatt ccgtcgccct
      601 tcgccaggac cgatgccgtc catccagggc ccttcctgat ggcatccgct tccagcgagg
      661 ccgagatgct ttcgtcatcc ttcgacattt caatgcggtg ccaccgtcca tcgagcatcg
      721 cctcgaaagc ggggcattcg catctggccg gattgaaccg gattgttacg ggctggagcc
      781 cgtcaagctg agccacggtg accttcaatc tcttcagtgc ctgttcggaa gcggaaagcg
      841 gcgcgccgac gcaaccgaga atggttgtac cgagaacggc aagcccgaca gcgattttga
      901 agccaggttt catccagtca gccgtaaacg acccagacga ccattgccgc atatccgacg
      961 ccgttaatga tgaatatcgg atccctcagc atccggtctg tcgggcttcc gttctcgccc
     1021 ggccgtctaa ggatcctgat gaactcgacg atgccgatga ttacgaacgg gattgtcagc
     1081 ggcatgcggt ggtttccgaa gtaccccgac gtcctcccgg aaaacgcgta aaggccgtaa
     1141 gccccgattg taagcacggc aacgatggtc atgacgacgt caaggtgcct tacatcgtaa
     1201 tcccgaagga cgtccctggt gtcggaacct tccagcaccg ccgcctcgtg ccgccttttg
     1261 gcaagggcga ggaacgacgc gatcaggaat gtgcatgcaa tcaggaagta ggaaatttcc
     1321 gccggggcca ggctgatggc aaaacacccg gccaggatcc gcagaaggaa tccggtggcg
     1381 atgcacagga catcaacata gacgaccttc ttcaggcgcg tgctgtatgc gaaattcagg
     1441 acaagatacc cggaagcggc aagcaggaag cgccatccaa ggaagtaccc gaccaggtac
     1501 gaagccgcaa gtgccagccc cgcaccgact gccgcattgg gaaccgccag ctttccggcc
     1561 gccagcggtc tgttcttctt ggtcgggtgc tgccgatcct tttcgagatc cagtatgtcg
     1621 ttgaatatat agaccgcgcc ggccaggagg atgaagacga tggtggcggc agtcgcataa
     1681 aggatcagtg aaggatcctt cctggtgtgg tcgcttgcga acacgaccgg ggcgaagacg
     1741 aagacgttct tgacccactg ggccggtcgc atggtggcga cgatctgcct ggcctggcta
     1801 atcatccgaa tcccctgtcg tgccgtcgta tccacgggac gcggccagca ccgcatcggg
     1861 aatggaatcg atcagatccg atgccgtcgt cccgtgttcg ccgaaccgcc gggatgcaac
     1921 ccttccggca atcgcatgaa ccgtcacggc ccagcaggcc gcgtcaaaag gtggtatgcc
     1981 ccttgccaga agcgcaccgg caatcccgga aaggacatcg ccggatcccg ccacggccag
     2041 cgcgggtgtt ccaaggtcgc agatggccag ccgaccgtcc ggattcgcga taatcgtgcc
     2101 ggcccccttc agg
//
LOCUS       DAOPPA010000521         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_56801_length_2113_cov_3.446550, whole genome shotgun sequence.
ACCESSION   DAOPPA010000521 DAOPPA010000000
VERSION     DAOPPA010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56801_length_2113_cov_3.446550"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKH54_12725"
     CDS             complement(<1..288)
                     /locus_tag="PKH54_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOD00803.1"
                     /translation="MNLNELKLMKITDLLNLAKDLNVENARGLRKQELIFAILQAQTE
                     QNGFIYGEGVLQVLPEGFGFLRAPDTSYLPGSDDIYVSPSQIRRFNLRTGDT"
     gene            complement(600..1661)
                     /locus_tag="PKH54_12730"
     CDS             complement(600..1661)
                     /locus_tag="PKH54_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00804.1"
                     /translation="MKHFQAFVMVAMTLAGCGGTEGSDIDASLADVALDSISEVEEIA
                     SDIPDPCRDAASPADRGPFAVVQGSHELSLTVEGDTRAVPVTFFLPDVPDPAPLVIFT
                     HGFSQEPAGYLGTGEYIASWGMVVVMPKIPGSAFSPVPHRVMAEYLRGVMDWAAGEGN
                     AVEAPLAGKVDAQSVGLSGHSMGGKLSFLLAASDERVDAVFGVDPVDAGPPIDDYDPA
                     DWPSVTPELMPEIQIPIVALGQTVDGELSDQLSCAPYGNRFSDYYDAAVGPAVMIEMV
                     GADHVSFLDTCDEFCSLFCRPGTDDPAVTLALTRKYLAAFFRHELMAESCARDWLAGD
                     QMDDDVAAGLVLAESKNGF"
     gene            1723..>2113
                     /locus_tag="PKH54_12735"
     CDS             1723..>2113
                     /locus_tag="PKH54_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00805.1"
                     /translation="MNRYGLCCLVLIAAAALGCEEEVAGELDASEPEVLVSDVPCDST
                     ACTDLGTVTTGKCHIALECPDNEYCFAPGQPMCGICMEPESTCGTDADCTEAGLVCDW
                     VRSTTCLCGPYKSCGPRCDAEDGVPCDP"
BASE COUNT          454 a          638 c          631 g          390 t
ORIGIN      
        1 cgtgtcgccg gtacgcaggt tgaaacgacg aatctgcgac ggggagacgt agatgtcgtc
       61 agacccgggg aggtagctgg tatccggggc gcgcagaaag ccgaagcctt ccggcagcac
      121 ctggagcacg ccttccccgt agatgaagcc gttctgttcg gtctgggcct gcagaatcgc
      181 gaaaatcagc tcctgcttgc gcagtccgcg tgcgttttcc acgttcagat ccttggccag
      241 attcaacaga tccgtaatct tcatcagctt gagttcgttg aggttcactt atttcctccg
      301 cacttatttt ttcggcgcaa aagttttatg aagggcggta ccgcgcgcca tttcggtccc
      361 gcttcatgga accaggcgaa tgccccgtcc cgaaacgaca aagtaaaaaa agtggaacta
      421 tcgaccagac agccgtgaaa tcaaagtatt acacgcgcct gaatcgtgaa aaccgatacc
      481 ttgaagaaaa agcgggaacg ggtggacagc ccggcccagt ggaacggttc agccggagtt
      541 tatgtttacg gatatccgat gtcaagactt tccgacattt ccgtgtcgaa ggtgaccggt
      601 cagaagccgt ttttcgattc ggcgagcacc agccccgcgg ccacgtcgtc atccatttga
      661 tcaccggcca gccagtcgcg ggcacacgac tccgccatca gctcatggcg gaagaaggcg
      721 gcaaggtact tgcgggtcag ggccagcgtg accgccgggt catccgttcc gggacggcag
      781 aacaggctgc agaactcgtc gcaggtgtcc aggaacgaaa cgtgatcggc gccgaccatc
      841 tcgatcatga cggccggccc gaccgccgcg tcgtaatagt cggaaaagcg gttgccgtat
      901 ggggcacacg aaagctgatc cgacagttcg ccgtcaaccg tctggccaag ggccacgatt
      961 ggaatctgaa tttcaggcat cagttccggc gtcaccgaag gccagtcggc cgggtcgtag
     1021 tcgtcgattg gcggcccggc atccacggga tccacgccga agacggcatc gacacgttcg
     1081 tccgacgccg ccagcaggaa cgaaagcttg ccgcccatcg agtgtccgga cagaccgacc
     1141 gattgcgcat caaccttacc ggccagcggc gcttcgacgg cattgccctc gcctgccgcc
     1201 cagtccatta ccccgcgcag gtactcggcc atcacgcgat gaggcaccgg actgaaggcg
     1261 ctgcccggta tcttcggcat aacgacgacc atgccccatg acgcaatgta ttcgccggtc
     1321 ccaaggtatc cggccggttc ctgcgaaaac ccgtgggtaa aaatgaccag gggtgccgga
     1381 tccggaacgt ccggcaggaa aaacgtcacg ggcaccgccc tggtatcgcc ttcgaccgtc
     1441 agcgacagtt cgtggctgcc ctggacgacc gcaaagggtc ccctgtccgc gggactggcc
     1501 gcatcccggc atggatcggg gatatcggat gcgatttcct caacttctga aatcgaatca
     1561 agggccacgt cagccaacga tgcgtcgata tcggaacctt cggtcccgcc gcagcccgcc
     1621 agcgtcatcg caaccatgac aaaagcctga aaatgcttca cagtatgtct ccggggacga
     1681 agttccataa aatatcacga acttttcggg gggaaggatt aaatgaacag atacggcctt
     1741 tgttgtctgg tacttatcgc tgcggctgcg ctgggctgcg aggaagaagt ggcgggcgaa
     1801 cttgacgcca gtgaacccga ggtgcttgtt tccgacgtgc cctgcgattc gaccgcctgc
     1861 accgacctgg gaaccgtgac gacaggaaag tgccacatcg cgcttgaatg tccggacaac
     1921 gagtactgct tcgccccggg acagccgatg tgcggcatct gcatggaacc ggaatcgacc
     1981 tgcgggacgg acgctgactg taccgaagcc ggccttgtct gcgactgggt gcgctcgacg
     2041 acgtgcctgt gcggtccgta caagtcatgc ggcccacgct gcgacgcgga ggacggagtg
     2101 ccgtgcgacc cgg
//
LOCUS       DAOPPA010000522         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_57443_length_2096_cov_3.284174, whole genome shotgun sequence.
ACCESSION   DAOPPA010000522 DAOPPA010000000
VERSION     DAOPPA010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57443_length_2096_cov_3.284174"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..>1795)
                     /locus_tag="PKH54_12740"
     CDS             complement(155..>1795)
                     /locus_tag="PKH54_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00806.1"
                     /translation="SALTVNDMCVLVGETCTCQGIAVTDPCGDVLNPECKVVKCDDGD
                     AWTTNDLCAEDEDGNCSCVGTPVTNPCWDGVNPSCKPYLCDDGNPDTISDRCVTSEDD
                     CLCVGTPITSACDDEINPKCSAVLCDDGSAQTINDKCVLVTVGEITLCQCRGTPVLSP
                     CSEILNPTCEAVACDDGDPTTRRDKCANDGTRDCNCVGSPITDPCGKELNPECRQVLC
                     DDRSVYTTNDRCQFTKEGCICVGDEITDPCSDVLNPECKPSLCDDGDDLTENDSCVLK
                     GEVCTCMGTEITDPCSDVLNPRCEPVRCDDGDSGTINDLCVGDLISGCVCEGVSNDPC
                     GLALNPSCKPVLCDDGLKWTIDDKCVSADACGCAGTIVREPCLDKYNPTCEQVACTTS
                     EGFDGECRHSLTTGCDCEKVSVGGCETVYDCMWLEWLADCYGYWTCQKNMCFPVCDKE
                     FCGDGKCDIAIGETVQTCAQDCVACDGPDDCGKSGFCLKEVGNCKGTGGFCQVKPEDC
                     PRLWAPVCGCDGTTFDNNCFAYMADMPIEHDGECKPIL"
     assembly_gap    1796..1805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1806..>2096)
                     /locus_tag="PKH54_12745"
     CDS             complement(<1806..>2096)
                     /locus_tag="PKH54_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00807.1"
                     /translation="SCEAVTCDDCNPLTTNDRCMQNAAMTCECGGDPIMDVCDFGLNP
                     ECNPDPCDDGSVLTMDDKCVTFGDICLCMGSAIEDPCSSVLNPECVDKKCDD"
BASE COUNT          419 a          674 c          570 g          423 t
ORIGIN      
        1 accccggacc ccctgattac gaatcaagtg ctctgccgac tgagctacgt tggccttgcc
       61 gtaaggcaga ggggattttc ccggaaatca gcgaaaagtc aatagttgaa aggaatacgg
      121 atgggcggcc acacggcgtt aatacgcgtc gtacctacag gatcggcttg cactcgccgt
      181 cgtgctcgat cggcatgtcg gccatgtacg cgaagcagtt gttgtcgaac gtggtgccgt
      241 cgcagccgca gaccggggcc cacaggcgcg ggcaatcttc cggcttgacc tggcagaaac
      301 cgcccgtgcc cttgcagttg ccgacttcct tcaggcagaa accggacttg ccacaatcgt
      361 ccggcccgtc gcaggcaacg cagtcctggg cacaggtctg gaccgtctcg ccaatggcga
      421 tgtcgcactt cccgtcgccg cagaactcct tgtcacacac cgggaagcac atattcttct
      481 ggcaggtcca gtacccatag cagtcggcca gccactccag ccacatgcag tcatagacgg
      541 tttcgcaccc gccgacggac accttctcgc aatcgcatcc ggtggtcagg ctgtgcctgc
      601 attcaccatc aaaaccctcg gacgtggtgc acgcaacctg ctcacaggtc gggttgtact
      661 tgtccaggca gggctcgcga acgatcgtcc ccgcgcatcc acaggcatcc gcagatacgc
      721 atttgtcgtc aatcgtccac ttcaggccgt cgtcacaaag aaccggctta caggacggat
      781 tcaacgccag accgcaagga tcgttcgaaa cgccctcgca gacgcaaccg gaaatcaggt
      841 cgccgacaca caggtcattg atcgtgccgg agtcgccatc atcgcagcgg accggctcgc
      901 agcgcggatt caacacgtca ctgcacggat ctgtaatttc ggttcccatg caggtacata
      961 cctcgccctt cagcacgcat gagtcgtttt cagtcaaatc gtcgccgtca tcacacaggc
     1021 tgggcttgca ctcggggttc agaacgtcgc tgcagggatc ggttatctcg tcgccgacgc
     1081 agatgcatcc ttccttcgtg aactggcaac ggtcgttcgt cgtgtaaacc gacctgtcgt
     1141 cacacagcac ctgcctgcac tccggattca gttccttgcc gcacggatcg gtaatcgggc
     1201 tgccgacgca gttgcagtcg cgcgtaccat cgttcgcgca cttgtcgcgc ctggtcgtcg
     1261 gatcgccgtc gtcacaggcg actgcctcac aggtcggatt caggatttcg gaacagggcg
     1321 aaagaaccgg cgttcccctg cattggcaca gggtgatttc cccgaccgtg accagcacgc
     1381 atttgtcgtt gatcgtctgc gcgctgccgt catcacacag cacggcgctg catttcgggt
     1441 tgatttcatc atcgcacgcg gacgtaatcg gtgtgccgac acacagacaa tcgtcctcgc
     1501 tggtaacgca acgatcgctg atggtgtcgg ggttgccgtc gtcacaaaga tacggcttgc
     1561 aggaaggatt gacgccgtcc cagcacggat tggtgaccgg ggtgccgacg cacgagcagt
     1621 ttccgtcttc atcctcggca cacaggtcgt tggtggtcca cgcgtcgcca tcgtcacact
     1681 tgaccacctt gcattccgga ttcaggacgt caccgcacgg atcggtcacc gcgatcccct
     1741 gacaggtgca cgtctcaccg acgagcacgc acatgtcgtt gactgtcagg gcactnnnnn
     1801 nnnnncgtcg tcgcacttct tatccacaca ctcgggattc aatacgctgc tgcagggatc
     1861 ctcgattgcc gaacccatgc acagacagat gtcgccgaaa gtgacgcact tgtcatccat
     1921 cgtcagcacg ctgccgtcgt cgcacggatc ggggttgcat tccggattca agccgaaatc
     1981 acacacatcc ataatcggat cgccaccgca ctcgcaggtc atcgccgcgt tctgcatgca
     2041 acgatcgttg gttgtcaacg gattgcagtc gtcgcaggtc acggcctcgc acgacg
//
LOCUS       DAOPPA010000523         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_57703_length_2090_cov_2.507617, whole genome shotgun sequence.
ACCESSION   DAOPPA010000523 DAOPPA010000000
VERSION     DAOPPA010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57703_length_2090_cov_2.507617"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(443..1957)
                     /locus_tag="PKH54_12750"
     CDS             complement(443..1957)
                     /locus_tag="PKH54_12750"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HOD00808.1"
                     /translation="MKFVEPIRVAEYQEILPDPPEGACDKDTRNYIEAHHDVLWQRLF
                     RFDDEHFRSTRSHVFEFRGKRVFACNNVGVIRTGGLTIEILPKIDAPSGQSSQSPPQA
                     GGAWNLAQHNLLAMLAYTRKLQIVARDIAALDIRKMSLLEVLIHTFATSLLDELVRGV
                     DRSYVAREDNLAFFKGKLLVGHHIRQNSLRRDRFSLGYEEFDADTRINRILLATCRRL
                     LVFSGRSDSVRLLTQAIAHMDCVSDVRILPHHFDNLILNRNNQRFEVLLNFCRMVLLG
                     QSPAPRHGASETFCLLFPMEQLFEEFVATFLRRHCANPDFMEDGKGGCFCSSEDVFAQ
                     GVGVDKKYLVCAEREGGRRDFRLKPDIIIMGRDANGNVFPRLIVDTKWKCLLEVDRTR
                     ARKVAQSDMYQMYAYANRFNCADVILLYPSTPELEQLLYPTTSELEQRTIARSYVIES
                     DEPVCDNAGCVTPARPGNVPKRIHIRLVPLHFDLLTDSGRRQFGKALRRALEVG"
BASE COUNT          467 a          596 c          575 g          452 t
ORIGIN      
        1 catcgttgcc atcaagggcg attcttaccg gaagcacgaa gccaggcagg aacgtcgcgg
       61 ccagacggac gaccaggacg agtagaacct gaagggaaac tcgaaagggc caagggtgtc
      121 ggctatcgcc gacacccatc aagccatacc gaccacgatg tccagatttt tacgttttac
      181 tgcgagcggc aacactttcc tatcagacct accttccggc attcgatgca tccgacctgc
      241 tggacttcca attcgcccaa ctgccggtcg gcaccaacgc tgttgtgctt ctattggcaa
      301 cggacacttc cggcaatgct tcgatgctgg tcgcggcgtt tgagttgtga tggacagaag
      361 gtgccatcgt ctggatcagt gaccagatta ggcggagttc gtgtccatcg gccggggact
      421 ccctggccgc ccagggaagt gttcacccca cttccagcgc acggcgaagg gctttcccga
      481 actgcctgcg cccactgtca gtcagaaggt cgaaatgcag gggaacaaga cgaatatgga
      541 ttcgctttgg gacgtttccc ggcctggcag gggtgacaca acccgcgttg tcgcagacag
      601 gttcgtccga ttcgatgacg taagacctgg cgatggtccg ttgttcaagc tccgaggtcg
      661 tggggtacaa caattgttca agctccggcg tcgaggggta caacaatatg acgtcggcgc
      721 agttgaaacg gttggcgtag gcgtacatct ggtacatgtc ggattgggcg actttgcgcg
      781 cgcgcgtccg gtccacttca agcaggcatt tccactttgt gtcaacaatc agccttggga
      841 acacgttccc attcgcgtcc cgccccataa tgattatgtc cggcttcagg cggaagtcgc
      901 gccgtccccc ttccctttca gcacacacga gatacttttt gtcgacgcca acgccctggg
      961 caaacacatc ctccgatgaa cagaagcacc cgcccttgcc atcttccatg aaatcagggt
     1021 tcgcgcagtg ccgcctcagg aacgtggcaa cgaactcctc gaagagctgc tccatcggga
     1081 acaaaaggca gaaagtttcg cttgcgccgt gccgtggggc gggagactga ccgagcagca
     1141 ccatgcgaca gaagttaagc agtacctcaa accgctggtt gttacgattc aggatcaggt
     1201 tatcaaagtg gtgcggaaga atcctgacat cggaaacgca atccatgtgt gcaatggcct
     1261 gggtcagaag gcgcacggaa tccgaccgcc cagaaaacac gagcaagcgc cggcaggtcg
     1321 ccaacaggat ccggtttatc cgggtgtcgg cgtcaaactc ctcgtatcca agcgagaacc
     1381 tgtcacggcg aagcgagttt tgccggatgt gatgtccaac cagtagcttt cccttaaaga
     1441 atgcgagatt gtcttccctt gcgacgtagc tgcgatccac cccccgaacc aactcatcca
     1501 acagggatgt agcgaatgtg tggatcagca cctcgagcag cgacatcttc ctgatatcca
     1561 acgcggcgat gtcgcgcgca acaatttgaa gttttcgcgt gtatgccaac atcgccagca
     1621 ggttgtgctg cgccagattc caggccccgc ccgcctgcgg gggggactgg ctggattgcc
     1681 cactcggcgc gtcgattttt ggcagaattt cgatcgttaa tccaccagtc cggatgaccc
     1741 cgacattgtt gcaggcgaaa actcgtttgc cgcggaattc gaatacatgg cttcgggttg
     1801 atcgaaaatg ttcgtcatcg aaacgaaaca aacgctgcca gagcacgtca tggtgggctt
     1861 cgatgtagtt tcgtgtgtcc ttatcacatg cgccttcggg cggatccgga aggatttcct
     1921 ggtattcggc gaccctgatt ggttcaacga acttcatacg tcgttccttg acacgatatt
     1981 gacgacaaaa aggccagctc catcgtcacg gccccggtca ttccgtcgcc tgttccgaat
     2041 ttgtggccac agttttcaga agaccggatt tgctgaaaaa tcctttgatt
//
LOCUS       DAOPPA010000524         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_57764_length_2088_cov_3.830300, whole genome shotgun sequence.
ACCESSION   DAOPPA010000524 DAOPPA010000000
VERSION     DAOPPA010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57764_length_2088_cov_3.830300"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKH54_12755"
     CDS             <1..292
                     /locus_tag="PKH54_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00809.1"
                     /translation="MVSYTVDDVSIQVTDVHFVPIEFLSWDCLTIGALGWGQIQIANA
                     NRVVVNANCSRRAWMLEFCDAMLHFYPKEIAKIGGRLSHFEAVRARWLAKVE"
     gene            complement(408..1448)
                     /locus_tag="PKH54_12760"
     CDS             complement(408..1448)
                     /locus_tag="PKH54_12760"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00810.1"
                     /translation="MLGGIFTFLIIVLVAGVMTVAWFGLGFAELLASVRDFFINTDIY
                     WITGSIAVAWLVAKIWNRITGAGTLGAFLQVCCIGFGLWFGLIPLINSNRTNADFKCT
                     NDFECNWNAQCVRDFCVVPFRDEDTFIGKSEFTQGSYKAWLTRTITDECDGIGNERKA
                     GECRRGVEDKVAARFQQVIDPARCGYLHDGDTPESALFINDDAPMPCIGKDDAAAICA
                     SLGGSLPTMDDYDWMFDDRKFSCKNTVMVGLDEFHGYAKVLKKREAENLMYGPGCGTG
                     KFRRVCDKAAHGNNEEGLCDAFGNLAEWTADGGSIGGAWWNTASTMREPQALTGPKSW
                     VGFRCVVPKDGI"
     gene            complement(1450..>2088)
                     /locus_tag="PKH54_12765"
     CDS             complement(1450..>2088)
                     /locus_tag="PKH54_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00811.1"
                     /translation="LHRECDKIPGGRVKDIEKRYDCNNAVDGKVDELFGTITSPERCQ
                     YFELAAQSGKALPTDDDAPMPCIGRDQAAQICSDLGGRLPTRKEYQSMFEDREFSCAN
                     TVMSGLDQFNGLVRRLRAREIRRMKDGPGCGTGKFRKACEKYRDGNTAEGLCDVFGNV
                     AEWTADGGAVGGGFRSNAAAMRGEVAATGPQIDVGFRCIILDEHLVEDRGIF"
BASE COUNT          377 a          671 c          566 g          474 t
ORIGIN      
        1 gatggtcagt tacacagttg atgacgtgtc gattcaggtt actgacgtgc atttcgtacc
       61 gattgagttc ctttcctggg attgcctgac cattggggct cttggttggg gccagatcca
      121 gatcgccaac gccaacaggg tcgttgtaaa tgccaactgt tcacgtcgcg catggatgct
      181 tgaattctgc gacgccatgc tgcatttcta tccgaaagag attgccaaga ttggtggtcg
      241 cttaagtcat tttgaggcgg ttcgtgcaag gtggctcgcg aaggttgaat agtggtgttt
      301 ttcactccgt cccgatattc gatagcccac aactgaagcc gaccatgaac ctctggcctt
      361 gagtaagtcc ggcttggcgc ttgttgtcaa aaaagcagct ttttcttcta gatcccgtcc
      421 ttcggcacca cgcacctgaa gccgacccag gatttggggc cggtaagggc ctggggttcg
      481 cgcatcgtgg atgcggtgtt ccaccaggcg ccgccgatgg agccgccgtc agcggtccat
      541 tctgccaggt tgccgaatgc gtcgcacagg ccttcttcgt tgttgccatg ggcagccttg
      601 tcgcagaccc tgcggaactt gcccgtgccg catccggggc cgtacatcag gttttcggct
      661 tcgcgcttct tcaggacctt cgcgtatcca tggaattcat ccaggccaac catcacggtg
      721 tttttgcaac tgaacttcct gtcgtcgaac atccagtcgt agtcgtccat ggttggcaga
      781 ctgccgccaa ggctggcgca aatcgccgcc gcgtcgtcct tgccgatgca cggcatcggg
      841 gcgtcgtcgt tgatgaaaag ggccgactcg ggcgtgtcgc cgtcgtgaag atatccgcac
      901 cgcgccgggt cgataacctg ctggaacctg gccgcgacct tgtcttcgac cccgcgccga
      961 cactcgccgg ccttccgttc gttcccgatc ccgtcgcact cgtccgtgat cgtcctggtc
     1021 agccaggcct tgtaactgcc ctgggtgaat tcggacttgc cgatgaacgt gtcttcgtcc
     1081 cgaaacggca ccacgcagaa gtctcttaca cactgggcgt tccagttaca ttcgaaatcg
     1141 ttcgtgcact tgaagtccgc gttcgtccgg ttggaattga tcagcggaat cagcccgaac
     1201 cacagaccga agccgataca gcagacttgc aggaacgccc ccagggtgcc ggcgccggta
     1261 atcctgttcc agatcttcgc gaccagccac gcgaccgcga tggacccggt tatccagtaa
     1321 atgtcggtgt tgatgaaaaa gtccctgacg cttgccagca gctcggcgaa tcccagtccg
     1381 aaccaggcga cggtcatcac cccggcgacc agcacgatga tcaggaatgt gaagatgccg
     1441 cccagcattt cagaagatcc ccctgtcttc gaccaggtgc tcatccagga tgatgcacct
     1501 gaacccgacg tcgatttgcg gaccggtcgc cgccacttcg ccccgcatcg ccgccgcgtt
     1561 cgacctgaac ccgcccccca cggcgccacc gtccgcggtc cattccgcga cgttcccgaa
     1621 cacgtcgcac agtccctcgg ccgtgtttcc atcgcggtac ttttcgcacg ccttcctgaa
     1681 cttcccggtg ccgcatccag gcccgtcctt catacgccgg atttcccgcg ccctgagcct
     1741 gcggaccagg ccgttaaact ggtccagccc cgacatcacc gtgttcgcgc atgaaaattc
     1801 gcggtcttcg aacatcgact ggtattcctt gcgtgtgggc agtcggccgc ccaggtccga
     1861 acatatctgc gccgcctgat cccggccgat acatggcatc ggcgcatcgt cgtccgtcgg
     1921 caaagccttt ccagactggg ccgcaagttc gaagtactgg cagcgttctg gcgaggtgat
     1981 cgtcccgaac aattcgtcta ccttcccgtc aaccgcgttg ttgcagtcat atctcttttc
     2041 aatatccttg acccgcccac cggggatttt gtcgcactcc ctgtgcaa
//
LOCUS       DAOPPA010000525         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_57838_length_2086_cov_3.882324, whole genome shotgun sequence.
ACCESSION   DAOPPA010000525 DAOPPA010000000
VERSION     DAOPPA010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57838_length_2086_cov_3.882324"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKH54_12770"
     CDS             complement(<1..542)
                     /locus_tag="PKH54_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD00812.1"
                     /translation="MKTQEELNLMFDPTRSVIVVAGAGTGKTYNLVRRYLTLIATSNP
                     KTGRPWSSPSEVLAITFTRAAAAEMKSRVISLLRRAADAEGPADFTQNGEIDHVMVKV
                     VENRPDYDELIAGIDAAPIDTIHGLCARLLREFPELSGVPVDARPVEPGEEAVLQGRF
                     LSGFLDRLLDRLDADDSGVQV"
     gene            complement(539..>2086)
                     /locus_tag="PKH54_12775"
     CDS             complement(539..>2086)
                     /locus_tag="PKH54_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD00813.1"
                     /translation="TGDLGHRVAAVSSHFNTAEPGPWTGELGVAFKPFYHSVSALQKY
                     NRLPYEYFLERVLGVSDQTESDDSPEAMEQGSLVHSALESSTIGKFDSDGLVDVSAVR
                     ADLGTAVCGGVSERFRSHFDGVLAMPVWNGDAARWAYELDLWWADFLNRMDNGPVEDP
                     REMPAFKAALEGSVLVWVANNDYPSFESWHRDLSAACRRITSAAATGTGGAGIRRSLS
                     GLEGKLDLLIPVANWLKFMASPDDPRWRDDNVQEESFGNTKMSSRKAIKAHFNPRPDL
                     KSDPIEGWIKKLHKDVLDILGRVRTVVAQLEPSYLAVPGSYLAGVEFKLGSKTQPLPL
                     KLSDDITIMMSGSIDRLEFDRTRKRLAVCDYKTGRPVSSGELLGKIRSGEHLQLPLYA
                     VAVDQMVDESLPHLNGFRVGAIRLEAMKRKPDGDRPVCAVRPDGIDDETGMKIVDIAK
                     ARAAQAVAAIESGRFPVELRGSVHKKRSRSMEAARIVGKGCPPALEGCGALENSADGS
                     GADEVVE"
BASE COUNT          445 a          631 c          564 g          446 t
ORIGIN      
        1 acctgaaccc cggaatcgtc tgcatccaga cggtcaagaa gacgatccag gaaaccactg
       61 aggaaccgtc cctgcagaac cgcctcttca ccgggttcaa caggccgcgc gtccactggg
      121 acgccggaga gttcagggaa ttcacgcaac agtcgcgcac acagtccatg gatcgtgtcg
      181 atcggcgcgg cgtcgattcc ggcgatcagt tcgtcataat caggccggtt ttcaacgacc
      241 ttcaccatga cgtggtcaat ttccccgttc tgtgtgaaat cggccggccc ctctgcatcg
      301 gccgccctgc gaagcaggga aatcacacgg gatttcattt cggccgcggc cgcgcgcgtg
      361 aacgtaatcg ccagcacctc tgacggactg gaccacggcc tgcctgtctt cgggttgctg
      421 gtggcgatca aagtcagata gcggcgaacc agattgtagg ttttgccggt tcccgcgcca
      481 gcaacaacaa tcaccgagcg ggtcggatca aacatcaaat tcagttcttc ctgcgttttc
      541 attccaccac ctcgtccgca cctgaaccat cggcactgtt ttcaagggca ccgcagcctt
      601 cgagtgccgg tggacacccc ttcccgacaa ttctggcggc ttccattgac cgtgagcgtt
      661 tcttatgcac cgaaccgcgg agttcaacgg ggaatcgacc ggattcaatc gctgcgacag
      721 cctgggcggc cctggctttc gcgatatcga caatcttcat ccctgtttcg tcgtcgatgc
      781 cgtcagggcg aacggcgcat acgggacggt ccccatcagg ctttctcttc atggcctcca
      841 ggcgaatggc gccaaccctg aaaccattca ggtgcggcag cgattcgtcg accatctggt
      901 cgacggcaac ggcatacagg ggcagttgca gatgttcacc ggatctgatt ttgcccagca
      961 gttcccctga tgaaacgggc cttccggttt tgtaatcgca tacagcgagt cgcttcctgg
     1021 tccggtcgaa ttcaagcctg tcgatgctgc cggacatcat gatcgtgata tcgtcggaca
     1081 gcttcagggg aagcggctgg gttttcgacc cgagcttgaa ttcaacaccc gcgagatacg
     1141 aaccgggtac tgccagatag gacggttcga gttgtgcaac tacggtgcga acccggccaa
     1201 ggatatcaag gacatcctta tgcagtttct tgatccagcc ctcgatcggg tccgacttca
     1261 aatcaggccg tggattgaaa tgagccttga tggccttgcg cgatgacatt ttggtatttc
     1321 cgaagctttc ttcctgaaca ttgtcgtcgc gccatcgggg atcgtcggga ctggccataa
     1381 atttcagcca gttcgccaca gggatcaaca gatccagttt tccttcaagt cccgagaggc
     1441 tgcgtctgat gccagcgccg cccgtaccag tggctgcggc tgatgtaatc cggcgacatg
     1501 ctgccgacaa atcgcgatgc catgattcga aacttggata gtcgttattc gcaacccaga
     1561 ccaacacact gccttcgagt gcggctttga aggctggcat ttcccgtgga tcctcgacag
     1621 ggccgttatc catccgattc agaaaatcgg cccaccacag gtccaattca tacgcccacc
     1681 tcgccgcgtc accgttccag accggcattg ccaggactcc atcaaaatgt gacctgaacc
     1741 gttcactgac gccaccgcag acagcagttc caagatcagc acggactgcg gacacatcga
     1801 caagtccatc cgaatcgaac ttgccaatgg ttgacgattc cagtgccgag tgaaccaggc
     1861 tgccctgttc catggcctcg ggagagtcat cggactcagt ctggtcggaa accccgagca
     1921 ccctttccag gaagtactcg tatggaagcc ggttgtattt ctgcagcgcg gaaaccgaat
     1981 ggtagaaagg cttgaatgca acaccgagtt cgccagtcca gggtcccggt tcggcggtgt
     2041 tgaaatggct ggaaaccgca gcgactcggt ggccaaggtc gcccgt
//
LOCUS       DAOPPA010000526         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_58490_length_2069_cov_2.843595, whole genome shotgun sequence.
ACCESSION   DAOPPA010000526 DAOPPA010000000
VERSION     DAOPPA010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58490_length_2069_cov_2.843595"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PKH54_12780"
     CDS             <1..848
                     /locus_tag="PKH54_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOD00814.1"
                     /translation="GHRPLAEAIVTAGGVDTHVERDRRWQTYGGFPLVTKDDARYELL
                     DNLGFDAGYSEYRKGPVWVAYRVDAARLPDRFPRPRGFATDDRTRARVAHRDYTGTGY
                     DRGHMAPNHAIASRYGREAQLETFLMSNVTPQKPALNRETWRKLEEMEAGKWSQKLEE
                     VWVITGPVFDDERTYLGNGEAGIDIPDAFFKVVIDEAPFDSRMTVHEYSDRAVAPKRG
                     IRPWHRPRVLAFIVPQDVDGSEPLTEFLVSVDWIEQKTGLDLMPEVPDDVEEAIESEI
                     ADQLW"
     gene            complement(864..>2069)
                     /locus_tag="PKH54_12785"
     CDS             complement(864..>2069)
                     /locus_tag="PKH54_12785"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HOD00815.1"
                     /translation="VVARNADSNQFSPGLFSRFDWTEKDGRVYYCNTRFDAATAQEAA
                     GEAPADGADPENGGCVSFAWSRLNQVPSWDFLGVFKDEWGTSHTIRPDGWIQDFEGQE
                     SLFRIISVNAEKGYLVAENDGENQFSPGLFSRMDWVTFMDRLYFCHTRFDAPTAADAE
                     ASPAPERKHPDQSGCQGFAWTALDPLAEPMAEIIDAPGDTGQGTGDAAKAVNGVRGGG
                     RDAGGSDVFSVGFDLDANGYLVIGIKDRSIKDGPGSDFAIFENVFSLGDGGMFMDPAV
                     VLLSRDGLTWVPYPFDYTADDENAYSKRPSDWDGFAGVTPVMLNEETNDVNPFDQAAA
                     GGDHFDLANLDPGDPEAAAIRKYGFRILKIIPAAIVLNPDTGEAFVRDPMSNGPDIDG
                     VWARNLLAD"
BASE COUNT          390 a          626 c          645 g          408 t
ORIGIN      
        1 ccggccatcg cccgctggcc gaggccatcg ttaccgccgg cggcgtcgat acccatgtcg
       61 aaagggatcg gcgctggcag acttatggtg gatttcccct tgtcacgaaa gacgatgccc
      121 ggtacgagct tcttgacaac ttggggtttg acgcaggcta ttcggaatac cggaaggggc
      181 cggtctgggt tgcctacagg gtcgatgccg cgcgtcttcc ggatcgtttc ccgcgtcccc
      241 gcggctttgc cacggatgac agaaccaggg cgcgtgtcgc ccaccgcgac tacacaggca
      301 ccggttatga tcgcggacac atggccccga atcatgccat tgccagccgt tacggccggg
      361 aggcccagct cgagacgttc ctgatgagca acgtcactcc ccagaagccg gcgctgaaca
      421 gggaaacctg gcgaaaactg gaagagatgg aagccgggaa gtggtcgcag aagctggagg
      481 aagtgtgggt cataaccggt ccggttttcg acgacgaaag gacttacctc gggaacggcg
      541 aggccggcat cgacattcct gacgctttct tcaaggttgt gatcgacgag gccccgttcg
      601 attcaaggat gacggtacac gaatactcgg atcgggcggt cgccccgaag cggggcattc
      661 ggccgtggca ccggccccgt gtgcttgcat tcattgttcc gcaggatgtc gatggaagcg
      721 agccgctgac cgaattcctt gtcagtgttg actggattga gcagaagacc ggcctggatc
      781 tgatgccgga agtgccggat gatgtcgagg aagcaatcga gtcggagatt gccgatcagc
      841 tgtggtaggc aggtgccagg ggatcagtcc gccagcaggt tgcgagccca gactccatca
      901 atgtcgggcc cgttcgacat cgggtcgcga acgaaggcct cgccggtatc ggggttcagg
      961 acgatggcgg ccgggatgat cttcaggatc ctgaagccgt acttccggat tgccgcggct
     1021 tcggggtcgc cagggtcaag attggccaga tcgaagtggt cgccgcccgc cgcggcctgg
     1081 tcgaacggat tgacgtcgtt cgtttcctcg ttcagcatga ccggcgtgac cccggcgaat
     1141 ccatcccagt cggacggtct ttttgaatag gcattctcgt catcggcagt gtaatcgaac
     1201 gggtagggaa cccatgtcag cccgtcgcgc gaaagcagaa ccacggcggg atccatgaac
     1261 attccgccgt ccccgaggga aaatacgttt tcgaagattg cgaagtcgct gcccggcccg
     1321 tccttgatcg acctgtcctt gattccaatt acaaggtacc cgttcgcgtc aaggtcgaat
     1381 ccgacggaga agacgtccga gcccccggca tcgcgccccc cgccccgcac cccgttaacc
     1441 gccttggcgg catcgccggt tccctgaccg gtgtcgccgg gcgcgtcgat gatttcggcc
     1501 atcggctccg caagcggatc aagggctgtc caggcaaagc cctggcaccc ggactgatcg
     1561 ggatgctttc tctcgggtgc gggcgatgct tcggcgtcgg cggctgtcgg cgcatcgaag
     1621 cgtgtatggc agaagtacag cctgtccatg aacgtcaccc agtccatcct gctgaacagc
     1681 cccggactga actggttttc cccgtcgttt tcggcgacca ggtatccctt ttcggcgttg
     1741 acggagatga ttctgaacag gctttcctga ccctcgaaat cctggatcca tccatctggc
     1801 cggatcgtgt gcgaggttcc ccactcatcc ttgaagacgc caaggaagtc ccatgaaggc
     1861 acctgattca gtctgctcca tgcgaatgag acgcatccac cgttttcggg atcggccccg
     1921 tccgccgggg cctcgccggc ggcctcctgc gcggtcgcgg catcgaatcg ggtgttgcag
     1981 taataaacgc gcccatcctt ttcagtccag tcgaacctgc tgaacaggcc gggcgagaac
     2041 tggttcgaat cggcgttcct ggcgacaac
//
LOCUS       DAOPPA010000527         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_58584_length_2066_cov_5.926902, whole genome shotgun sequence.
ACCESSION   DAOPPA010000527 DAOPPA010000000
VERSION     DAOPPA010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58584_length_2066_cov_5.926902"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKH54_12790"
     CDS             <1..253
                     /locus_tag="PKH54_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00816.1"
                     /translation="GQTDIYGFCKGGWRCGTDDKCRPFEGAECLWVDWMGTCWADNWL
                     YQCSGDVPGQGSIIAENCTTSGKVCYFNVSAQQYKCGTL"
     gene            complement(357..1658)
                     /locus_tag="PKH54_12795"
     CDS             complement(357..1658)
                     /locus_tag="PKH54_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOD00817.1"
                     /translation="MLLESVFHIKERGSTVRREVSGGLTTFITLAYIIVVNPAILEAA
                     GIPKDASMAATIFTAFIGTMIMGVWANRPFAIAPYMGENAFVAFTVVKVLGFSWQTAL
                     GAIFLSGFLFSILTVLGVRKALVQSIPMGLKSSFALGIGLFLAFVGFNEIGLVRLGVP
                     GAPVTMGDVSSLPVVIGIATTFLMAVLMYWKVPGAILLAILGGATAAFATGVAPWPGA
                     IVSSPPSMAATFMQFDLKGAMSLSFLNVVLVVFVMDFVDTMGTLIGLSARAGMLDRDG
                     NLPQIERPMLADALSTVVAAILGTTTAGIFIESAAGIEAGARTGLAAVVTALLFLVAL
                     FFWPLLTAMPACAYGPALIIVGGMMLQAVSGIEIGRMDEFLPAFLVVVLMAFTYNIGI
                     GMTAGFVAWPILKIAAGKWRQVMPGMWVLAAISMAFFVFHL"
     gene            1828..>2066
                     /locus_tag="PKH54_12800"
     CDS             1828..>2066
                     /locus_tag="PKH54_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00818.1"
                     /translation="MFRNIVVAGVAVASLALIVAVSFDVGAKPKAGTKPAKVEQAANA
                     GELKGPVIIDKSFESFQFGITKAQAEEFVKGRVRA"
BASE COUNT          466 a          612 c          608 g          380 t
ORIGIN      
        1 tggccagacg gacatttacg gcttctgcaa gggcggctgg cgctgcggca ccgacgacaa
       61 gtgtcgtccg ttcgagggcg ccgaatgcct gtgggttgac tggatgggta cgtgctgggc
      121 cgacaactgg ctttaccagt gcagcggcga cgttcccggc cagggaagca tcatcgccga
      181 aaactgcacg acttcgggca aggtctgtta cttcaacgtt tccgcccagc agtacaagtg
      241 cggcaccctg taaatgcgaa tgaaaattcg cccgcccatt tccttgtacg ggcggattac
      301 atccgcccgc cgcggttctg cacgatccgt acgggcgaat gaaaattcgc ccgccatcac
      361 agatgaaaaa cgaagaatgc cattgaaatc gcggcaagca cccacatgcc cggcatgacc
      421 tggcgccact tcccggccgc gattttcaag attggccatg caacgaagcc ggcggtcatc
      481 ccgatcccga tgttgtacgt gaaggccatc agcaccacga ccaggaatgc cggcaggaat
      541 tcatccatgc gaccgatctc gattccggaa acggcctgga gcatcatgcc gccaacgata
      601 atcagcgccg ggccgtaagc gcacgcgggc attgcggtaa gcagcggcca gaagaacagg
      661 gcgaccagaa aaagcaacgc cgtgacgacg gccgcaagtc cagttcgcgc cccggcctcg
      721 atcccggccg cgctttcgat gaaaatcccg gcggtcgtcg ttcccaggat tgcggcaaca
      781 accgttgaaa gcgcgtctgc gagcatcggt ctttcgatct ggggaaggtt gccatccctg
      841 tccagcatgc cggccctggc cgacagcccg ataagggttc ccatcgtatc gacgaagtcc
      901 atcacgaaga caacaaggac gacattcaga aacgacaacg acattgcgcc cttcaggtcg
      961 aattgcatga acgttgccgc catggatggc ggactggata cgattgcacc cggccatggc
     1021 gcgacgccgg ttgcaaaggc ggctgtcgcc ccgccaagga tcgccagaag tatggccccc
     1081 ggcaccttcc agtacatcag tacggccatc aggaaggtgg tggcgattcc gatcaccact
     1141 ggcagcgacg acacgtcgcc catcgtgacc ggcgccccgg gaactccaag gcgaaccagt
     1201 ccgatttcgt tgaatccgac aaatgccagg aacagcccga ttcccagggc gaagctggac
     1261 ttcaatccca taggtatgga ctggacgagc gcttttctta cgccaagcac ggtcagtatt
     1321 gaaaacagga aaccgctgag gaatatggcg ccaagtgccg tctgccatga aaagccaaga
     1381 accttcacga cggtaaaggc gacaaaggcg ttttcaccca tgtagggcgc tatcgcgaac
     1441 ggccggttcg cccaaacccc cataatcatc gtgccgatga acgccgtgaa gatggtggcg
     1501 gccatcgacg cgtccttcgg gattccggcg gcctccagga tggccgggtt gacgacgatg
     1561 atgtacgcaa gcgtgatgaa ggtcgtcaat ccgccggaaa cttccctgcg aacggtcgaa
     1621 ccgcgttcct ttatatggaa aactgattca agcaagatga tcccccgttt cgtggctgtg
     1681 cccgaatcta tcaggaaaac gtctcccggg gaaccgccgg ccgcttggct gctgtagaat
     1741 gcgccgtata attgtccgat ttcttgcggg aatctgccga gagccttaca atgtctggta
     1801 agtatgccca ggctctggag gtgaatcatg tttcgcaata tcgtcgttgc cggcgttgcg
     1861 gtggcgtcac ttgccctgat cgtggctgtt tccttcgacg tcggtgccaa gccgaaggcc
     1921 gggacgaagc ctgcgaaggt cgaacaggcg gccaacgccg gggaattgaa gggccccgtg
     1981 attatcgaca agagctttga gtcattccag ttcgggatta ccaaggccca ggccgaggaa
     2041 ttcgtgaagg ggcgggtgcg cgcata
//
LOCUS       DAOPPA010000528         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_59440_length_2043_cov_4.162978, whole genome shotgun sequence.
ACCESSION   DAOPPA010000528 DAOPPA010000000
VERSION     DAOPPA010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59440_length_2043_cov_4.162978"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1202)
                     /locus_tag="PKH54_12805"
     CDS             complement(108..1202)
                     /locus_tag="PKH54_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00819.1"
                     /translation="MKKRAILAAFVSMMLSVPAAAIVINNSPDEPAMRPTGPADFMRH
                     PSKAKYYTESWTTILHSDEGHAIYVNFALTNIGVVKGSAGLNMSFTAPGKPSEHWAVE
                     HSLEDYSEDKAAGQIRIGPNTMTLKGKNLSVDIKDAGLELKLKMTGWHQNGYKFHDGK
                     TFVTEKKDKYFAHFFHVPRGDFEAEITWQGRTIKLKGAGYMDHMTSNRLTSEYTRRWW
                     TVRYFTPNYTLAFITMQLDPKFGGEIVTRAFFGSRTKALAVTDVMDLNPSRFAQDNKA
                     SHKYHTRFDVGMKKESFDFSATFQSRRLHDREAVVEGLPKVQQGIAKLFAGNPVVYRM
                     EGAAEMKVSEGGVAKTESGVALMETIVLEN"
     gene            complement(1274..>2043)
                     /locus_tag="PKH54_12810"
     CDS             complement(1274..>2043)
                     /locus_tag="PKH54_12810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOD00820.1"
                     /translation="IMTELLSYADRLRPMVADTGLLLSEALDRGETVLFEGGQANDPV
                     VECTCNCEGGGSATTFKGASFVMDQLKLGAPLVSFKDIVNDFIAGEMGKGTINVLLSV
                     LEDDRSSGIMDVRFGVGTKAGSGYSFDDGFADVEVALSAETGAFTFVDGVSRFVLSVP
                     MEEGDPISIPMQNVILTGNIAGGKITGGTLTAVVSRADADATVVAGFQLSSVLIDAEQ
                     VQPDYDLDQDGTMDAWVFNGTFTAKTATVTVEQAPAE"
BASE COUNT          403 a          630 c          576 g          434 t
ORIGIN      
        1 atccgcccgt acgggcgaat gaaaattcgc ccggggcaac gcacagaatc gccgcgggca
       61 catgaaatcc gcccgtacgg gcgaatgaaa attcgcccgc cagaccgtca gttttccagg
      121 acgatcgttt ccatcagggc aacgccgctt tcggtcttcg cgacgccacc ttcggaaacc
      181 ttcatctcgg cggcgccttc catgcggtaa acgaccgggt tgccggcgaa cagtttcgcg
      241 atgccctgct gaaccttcgg caggccctca accacggcct cgcggtcgtg caggcgccgt
      301 gactggaaag ttgccgaaaa gtcgaacgat tccttcttca tcccgacgtc gaacctggtg
      361 tgatacttgt ggcttgcctt gttgtcctgg gcaaacctgc tcggattcaa gtccatcacg
      421 tccgtgaccg ccagcgcctt ggtccggctg ccgaaaaacg ccctggtcac gatctcgccg
      481 ccgaatttcg ggtcaagctg catcgtgatg aaggcaagcg tgtagttggg ggtgaagtag
      541 cgaaccgtcc accagcgcct tgtgtattcg gacgtcaggc gattgcttgt catgtgatcc
      601 atgtagccgg cgcccttcaa cttgatggtt ctgccctgcc aggtgatttc ggcctcgaaa
      661 tcgccacgcg gaacgtggaa aaaatgcgcg aagtacttgt cctttttttc ggtcacgaag
      721 gtcttgccgt catgaaactt gtatccgttc tggtgccagc cggtcatctt cagtttcagt
      781 tcgagaccgg cgtccttgat atcgaccgaa aggtttttcc ccttcaacgt catcgtgttc
      841 ggaccgatcc ttatctgccc ggctgccttg tcctcgctgt aatcttcaag cgaatgctcg
      901 acggcccaat gctcgctcgg ctttcccggg gccgtgaacg acatgttcag cccggcgctt
      961 cccttcacga cgccgatatt ggtcagggcg aaattgacat agatggcgtg gccttcgtcg
     1021 gaatgaagga tcgttgtcca actctccgtg tagtacttgg ccttgcttgg atgcctcatg
     1081 aagtcggcag gtccggtcgg acgcatcgcc ggctcgtcag ggctgttgtt aataacgatc
     1141 gccgccgccg gaacggaaag catcatcgaa acaaacgctg ccaggatggc tcttttcttc
     1201 attgtcttca cctttgctaa actgcatgag tccggcaggc cgcccaatca ggcacgcgcc
     1261 aggccggtgt cgatcattcg gccggcgcct gctcgaccgt gacggtggcg gtcttcgcag
     1321 tgaaagtgcc gttgaaaacc caggcgtcca tggttccgtc ctgatccagg tcatagtccg
     1381 gctgaacctg ctcggcatcg ataagaaccg aggaaagctg gaacccggcg acgacggtcg
     1441 catcggcatc ggcacgggac accacggcgg tgagagtgcc gcccgtaatc ttgccgcccg
     1501 cgatattacc ggtcaggatg acgttctgca tcgggatgga aatgggatcg ccctcttcca
     1561 ttggaacgga cagcacgaat ctcgacacgc cgtcaacgaa cgtgaaggcg cctgtttcgg
     1621 ccgacagcgc gacctcgaca tcggcaaaac catcatcgaa agaatagcct gacccggcct
     1681 tggttccgac accgaacctg acgtccatga tgccggagga acggtcgtcc tccaaaacgg
     1741 acagaagcac gttgatggtg cccttgccca tctcgcccgc gataaaatcg ttgacgatgt
     1801 ccttgaagga taccaggggg gcccccagct tcaactgatc catcacgaag gaagcgccct
     1861 tgaacgtggt ggccgacccg cccccttcac agttgcatgt gcactcgaca accggatcgt
     1921 tcgcctggcc gccctcgaac agcacggtct cgccgcggtc gagcgcctcg ctgagcagca
     1981 ggccggtgtc ggccaccatc gggcgcaggc gatctgcata cgagagcagt tctgtcatga
     2041 tct
//
LOCUS       DAOPPA010000529         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_59935_length_2031_cov_4.507591, whole genome shotgun sequence.
ACCESSION   DAOPPA010000529 DAOPPA010000000
VERSION     DAOPPA010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59935_length_2031_cov_4.507591"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKH54_12815"
     CDS             <1..372
                     /locus_tag="PKH54_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOD00821.1"
                     /translation="DGTGTFSVGSKVIDGMKFDFGTRFGDVPGTNPEELIAASHAACF
                     SMAFSAQMGERGIVPEAVETGCVITMENLVLTKSELTTVVRAKGADKAKIEEAAAAAK
                     AGCPISRVLNLEIALDLTVLD"
     gene            complement(455..1450)
                     /locus_tag="PKH54_12820"
     CDS             complement(455..1450)
                     /locus_tag="PKH54_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOD00822.1"
                     /translation="MPRARGEGPDDPKALNLAKIVFKLLFEPRGINVASLMDELGIKE
                     RTFRSYRQTLDLMPQFQDSNGASLIQVAGRGSERRISLRPSAEKDLNESHFAIRYAAL
                     NYARQTFSFMSGTPIGEYMATFCNDYVAQVSNQTYVFNHLFRDGDRKFFYLPWAPKDY
                     SGRGRDVQTIIRALSDNFKLEVEYESSRNPAARVVLPLTLVMWKSALYLIVQSEDRRR
                     TYMMAVDRMRHVRTIPQKFKYPDRGSYMPASFLDGGFGVFVDNCGKPREFELMFQPDP
                     DLHRQVRERTWHRSQVFEDLPDGRMRMTFTVTTDVEVWPWIRSFGDAVEVIRPAR"
     gene            1720..>2031
                     /locus_tag="PKH54_12825"
     CDS             1720..>2031
                     /locus_tag="PKH54_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00823.1"
                     /translation="MSILRAGLVWVARFSFVLVVVAGLAGCGPTMKVSRVAVDETIDL
                     SGNWNDSDSRMVSSDLINDMLNQHWIEEFVANASAQSGQPTKPVVVVGRVYNNTDEHI
                     NP"
BASE COUNT          439 a          565 c          586 g          441 t
ORIGIN      
        1 gacggaacgg gcactttcag cgtcggcagc aaggtgatcg acggcatgaa gtttgatttc
       61 gggacgcgtt tcggcgacgt tcccggcacc aatcccgagg aacttatcgc cgcgtctcat
      121 gcggcctgct tcagcatggc gttttccgcc cagatgggcg agcgtggcat cgttccagaa
      181 gccgttgaaa ccggttgcgt catcacgatg gaaaacctgg ttctgacgaa gagcgaattg
      241 accacggtcg taagggcgaa gggcgccgac aaggcaaaga tcgaggaagc tgccgccgcc
      301 gccaaggccg gttgcccgat ttcgcgcgtg ctgaaccttg aaatcgcgct ggatctgacc
      361 gtacttgact gagcattcca tcgcctggtg tatgcgaacg accattcaca caccacgcac
      421 tgggccggag ttccgccggc gctgaatcgc gggttcaacg ggctgggcgg ataacttcca
      481 cggcgtcacc gaatgatcga atccagggcc acacctcgac atcggtcgta accgtgaacg
      541 tcatccgcat ccgtccgtct ggaagatcct cgaaaacctg tgatctgtgc cacgtccgtt
      601 cgcgaacctg ccgatgaaga tccggatcgg gctggaacat aagctcgaat tcccttggct
      661 ttccgcagtt gtcaacgaac acgccgaagc cgccgtcaag gaaggatgcc ggcatgtacg
      721 atccccggtc ggggtacttg aacttctgtg gaatggtgcg gacatgtcgc attcgatcaa
      781 ccgccatcat gtaagttctt cgtcgatcct cactttgcac gatcagataa agcgcgctct
      841 tccacatgac cagggtgagg ggaaggacga cgcgcgcggc aggattcctt gacgactcgt
      901 actcgacctc cagcttgaag ttgtcagaaa gcgcacggat gatggtctgg acatccctgc
      961 cgcgccccga gtagtccttg ggcgcccagg gcagataaaa gaatttgcga tcgccatccc
     1021 ggaagaggtg gttgaagaca tacgtctggt tgctgacctg cgcgacgtag tcgttgcaga
     1081 aagtcgccat gtattcgccg atcggcgtcc cggacatgaa ggaaaacgtc tggcgcgcgt
     1141 aattaagggc cgcgtaccgt atcgcgaagt gcgattcgtt cagatccttt tccgcgcttg
     1201 gccgcaggga aatgcgacgt tccgatccac ggccggcgac ctggataagc gaagccccgt
     1261 tgctgtcctg aaattgcggc atgagatcga gcgtctgtcg gtacgaacgg aatgtgcgct
     1321 ccttgattcc aagttcatcc atcagcgaag cgacgttaat gccccttggt tcaaagagaa
     1381 gcttgaagac aatcttcgca agattcaaag ccttcggatc gtccggacct tcaccgcgtg
     1441 cccttggcat gatgcacctc gaatggttgt cggcatatca tgtccagaaa gttcgaaccc
     1501 ggcaagaatc ttgccgcggt gggttgcatg atcctgactg aaatcccggg gccggcacac
     1561 ccccgggatt tcaccccttt aaccggccgc gccggtggct gaagtaattt ttcgaccaat
     1621 ttcgggcata ttgataaaac ccactaccaa gaagtgttaa cgtgtggtaa acgtgtcgtt
     1681 caagcgaggt atctttcaat ttgtcattca ggaggctgta tgtcgattct tcgcgctggt
     1741 cttgtgtggg tcgcaaggtt ttcttttgtt ctggtcgtgg tcgccggtct tgcgggttgc
     1801 ggcccgacga tgaaggtgtc cagggtcgcg gtcgatgaaa cgatcgacct ttccggtaac
     1861 tggaatgaca gtgattcccg gatggtttca agcgacctta tcaacgacat gctcaaccag
     1921 cactggatcg aggaattcgt ggcaaacgcc tcggcccaga gcggccagcc gacaaagccg
     1981 gtcgtggtcg taggccgtgt ctataacaac acggacgaac acattaatcc c
//
LOCUS       DAOPPA010000530         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_59985_length_2030_cov_3.247595, whole genome shotgun sequence.
ACCESSION   DAOPPA010000530 DAOPPA010000000
VERSION     DAOPPA010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59985_length_2030_cov_3.247595"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PKH54_12830"
     CDS             <1..1846
                     /locus_tag="PKH54_12830"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOD00824.1"
                     /translation="DLGFGDCRRQYLDDQGRVFRHVAVSYDEKSGSFLLNCVNVAHQT
                     LLSNLWEIFYRHLIDVDFVQVERVFDCAPRVRTYYRVVAHCVSGVSERDVVSVQADFE
                     RYIRHFTITMSLFDLVGPAMVGPSSSHTAGANRIGQIARAIILAKIEQGETVRSVGVR
                     LVGSFADTGVGHKTPVALGGGLCGFDADDEGMLSAGSIESLQSGGIDFGGCVARFDGF
                     VRGTDADLARYAEDRCGNIAEIIFRTDRGEYTVTGFSIGAGNVEVRYFNGRLVEPING
                     KHDFFLDGDRIVSRPDAGRCLPVIRRIFDAESPRDDYVLRFNSFEELIDYCRDHGFDL
                     LRVVMDVESAYQRATPEELEERMLMYWARMKASVKSGVHSRAKSMFGLSGDGAFRMGL
                     FLKENSLFDNLYGRAAAYATAVNEVNARGGVIVACPTAGSCGILPGVLVAYQEATGAS
                     DDRIVQALMISGFLGMLLFSDVSTSGAAYGCQAEIGSAAAMAAAALCFLEGGDVETTI
                     AGFILAIKNSLGLICDPVAGLVEVPCVKRNGIYSSVAITAAMMALSGVKSFVSPDEVI
                     LTMREVGEKLSSDYKETARGGLARTRDGKHVERMFAAEVRRFFGGSDD"
BASE COUNT          384 a          542 c          667 g          437 t
ORIGIN      
        1 cgaccttgga ttcggtgatt gccgcaggca gtatcttgac gatcaggggc gcgtgttccg
       61 tcatgtagcg gtcagttatg acgaaaaatc cggatctttc ctgttgaact gcgtgaatgt
      121 ggcccaccag acgctgcttt cgaatctgtg ggagatattt taccgccacc tgattgatgt
      181 cgatttcgta caggtcgaaa gggttttcga ctgcgcaccc agggtgcgta cgtactacag
      241 ggtcgtggcc cattgcgttt cgggggtttc cgaaagggat gttgtctcgg tccaggccga
      301 ttttgaacgt tacatccgcc acttcacgat cacgatgagc cttttcgacc tggtcgggcc
      361 ggcaatggtc gggccgtcga gcagccacac cgccggcgcc aaccgcatcg gacagattgc
      421 ccgcgccatc atcctggcga agatcgagca gggtgaaacg gttcgttcgg tcggtgtacg
      481 gcttgtcgga tcgttcgcgg acaccggggt cggccacaag acgccggtgg cccttggcgg
      541 tggcctctgc ggatttgacg ccgatgacga ggggatgctg tcggcgggat ccatcgaaag
      601 cctgcaatcc ggcggaatcg acttcggcgg gtgcgtcgcc aggtttgatg gattcgtgcg
      661 tggaaccgat gccgatctgg caaggtacgc cgaggatcgg tgcggcaaca tcgccgagat
      721 catattcagg acggacaggg gcgaatacac ggtgaccgga ttttcaatcg gcgccggcaa
      781 cgtcgaagtc aggtatttca atgggcggct ggtcgagccg attaacggca agcacgactt
      841 cttcctggat ggcgacagaa tcgtttcgcg acctgacgcc gggcgatgcc tcccggtcat
      901 ccggcgcatt ttcgatgccg agtcgccacg cgatgactac gtcctgcggt tcaattcgtt
      961 cgaggaactg atcgattact gccgggatca cggttttgat cttctgcggg tggtcatgga
     1021 cgtcgagtcc gcgtaccagc gcgcgacccc cgaagaactg gaagaaagga tgttgatgta
     1081 ctgggcgcgg atgaaggctt ccgtcaagtc gggcgtccac agccgcgcga agtcgatgtt
     1141 cgggctgtcc ggcgacggcg cttttcggat ggggcttttc ctgaaggaaa acagtctgtt
     1201 tgacaacctg tacggacggg cggccgctta cgcgacggcc gtgaacgagg tcaatgcccg
     1261 gggcggcgtg atcgtcgcgt gtccaaccgc cggctcgtgc ggaatcctgc ctggcgtgct
     1321 ggtggcatac caggaggcga ccggcgcatc cgatgaccgc atcgtgcagg ccttgatgat
     1381 ttcggggttc ctggggatgc ttctcttttc cgatgtttcg acatccggcg cggcgtatgg
     1441 ctgccaggcc gaaatcggat ccgccgcggc catggcggcg gcggctctgt gctttcttga
     1501 aggcggggac gtcgaaacga cgattgccgg tttcatcctt gcgataaaga acagcctggg
     1561 acttatctgt gatccggtcg ccggcctggt cgaggttccg tgcgtcaaga gaaacggcat
     1621 ctattcgtcg gttgcgatta cggccgcgat gatggccctt tccggcgtga agtctttcgt
     1681 ttcgccggac gaggtcatcc tgacgatgcg agaggtcggg gaaaaactga gcagcgatta
     1741 caaggaaacg gcccgcggcg ggcttgccag gactcgcgac ggcaagcatg tcgaaaggat
     1801 gttcgccgcg gaagtccgca ggttctttgg cggttcggac gattgaaccg tcagcccgcg
     1861 cctgaataag cactgagcga tcccctgata gagagacgag gtattttgga gcccgcgcca
     1921 gcactggagt aagttgcgat tgacacagaa cgctgtgtaa ttgtcgttaa gcatgtggaa
     1981 tcgcggaaga attcctcgat ggtaaagcgc ctgccgtaaa gagcgacgat
//
LOCUS       DAOPPA010000531         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60145_length_2026_cov_11.888889, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000531 DAOPPA010000000
VERSION     DAOPPA010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60145_length_2026_cov_11.888889"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKH54_12835"
     CDS             complement(<1..481)
                     /locus_tag="PKH54_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00825.1"
                     /translation="MDFREDNREVRIAAGCSLACPFCTLGEGREGFAAADVIAGRIDL
                     PEADRYILLAGNLIDETHVPLVARLKAAGAAAIDLYAHSGVRNPAAMKALADAGMTGI
                     RLVLPAATREDLASMTNGRGSLKTAATLLAGAAALGLEVTLEVPVTGKNAGLTADTVT
                     "
     gene            complement(513..>2026)
                     /locus_tag="PKH54_12840"
     CDS             complement(513..>2026)
                     /locus_tag="PKH54_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00826.1"
                     /translation="CGPRGWDYRLAAGQVIEAVELARTAGVPVRTGGREGAPPCVLDI
                     RGATADLYPELDRLAIDITARPFATCASCDAAGACVADAPGFDPAPDRPLEPVLRQGA
                     CVATVPSETPENRLSPESSAALFIRRTDLNRLLVAAAKVNRCTAPWDSLEAHDRTGRV
                     APCEGSWPRRDVVGDVPNHGCGWMNDGLLRSFNSPAMVRMRRTMMASGREASCNPHCP
                     RFGESAENRPQIKLPRTQVFHDNLLRNLTEFVDGADILESRPRNITISPSLRCNQRCR
                     MCDLTDSLAGETGIDTPPTIIDELMELLPTTATLALTGGEPLISSRIVKLIAGITDAR
                     LPDTSVTLTTNGTLLTPTVIGALKASRISKVYISLNASNQESHTRVTGIDGRFQKVLN
                     NIAAMVAASAFMPLRPSVILSFVVMKSTLTDLPEFIELARRQGAGIRLLPVERNRLDE
                     SIFTDPDTMLHAHKALMKLSQAYTDTWPEAAAETDKLVALLKKRIDSNELAPL"
BASE COUNT          361 a          610 c          648 g          407 t
ORIGIN      
        1 tggtgaccgt gtctgccgtg agaccagcgt tctttccggt gaccggaact tcaagcgtta
       61 cttcgagacc gagcgccgcg gccccggcga gcagtgtcgc tgcggttttc agggagccgc
      121 gtccattggt catggaggcc agatcctccc tggtggcggc cggcaggaca agcctgatcc
      181 cggtcatgcc tgcatccgcc agcgctttca tcgcggcagg attccttacc ccggagtggg
      241 cgtagagatc gattgccgcg gcaccggcgg ccttcagcct ggcaaccagc gggacatgcg
      301 tttcgtcgat caggtttccg gcaagcaaga tgtaacgatc ggcctcgggc agatcgatcc
      361 ttccggcaat cacatcggcg gcggcaaaac cttcacgtcc ttcgcccagg gtgcagaaag
      421 gacatgccag cgagcaaccg gccgctatcc tgacctcacg gttgtcttcc cgaaaatcca
      481 tctgtcaaaa tccccggcgg ttggcgtcgc ggctagagcg gcgccagctc gttcgaatcg
      541 atccgttttt tcaacagcgc gaccagtttg tctgtctcgg cagcggcctc cggccaggta
      601 tccgtgtatg cctgactcag cttcatcaat gccttgtgcg catgcagcat cgtgtccggg
      661 tcggtgaaga tgctttcgtc aagcctgttc cgttccaccg gcagcaacct tatcccggcg
      721 ccttgccgcc gcgcaagctc gatgaattcc ggcaggtccg tcagggtgga tttcatgacc
      781 acgaaactga gtatcaccga tgggcgcaga ggcatgaaag ccgaggccgc taccatggcc
      841 gcgatgttgt tcagcacttt ctggaatcgg ccatcgatgc ccgtcacacg ggtgtgtgat
      901 tcctggttgg acgcattcag cgatatgtag acctttgaaa tcctggatgc cttgagtgcc
      961 ccgatcaccg tcggagtcag cagcgtgcca ttggtagtca gcgtgacgct tgtatcgggc
     1021 agtctggcgt cggtgatgcc ggcgataagt ttcacgatac ggctggagat gaggggttcg
     1081 ccaccggtca gggcaagggt cgccgtggtc ggcagcagtt ccatcagttc atcgatgatc
     1141 gtcggtggcg tgtcgatgcc ggtttcaccg gcaagggagt ctgtcaggtc gcacatcctg
     1201 cagcgctgat tgcatcgcag tgatggcgag atcgtaatgt ttcgcggtcg tgattccagg
     1261 atgtcggcgc cgtcgacgaa ctccgtcaga ttgcgcagca ggttgtcgtg aaagacctga
     1321 gttcgcggca gcttgatctg ggggcggttt tcagccgact ccccgaatcg cgggcagtgc
     1381 gggttgcagg atgcctcgcg accgctggcc atcatcgtgc ggcgcatcct gaccatggcg
     1441 ggagaattga acgagcgcag aagaccatcg ttcatccatc cgcatccgtg gttcgggacg
     1501 tctccaacca cgtcgcgtct gggccatgag ccttcacacg gtgccacgcg cccagtcctg
     1561 tcatgtgctt ccagggaatc ccagggcgcc gtgcagcggt tgacttttgc agcagccacc
     1621 agcaggcggt tcagatcggt tctgcgaatg aaaagggcgg ccgaggactc gggcgacaaa
     1681 cgattttctg gtgtttcgga aggaacggtc gccacgcatg ctccctgtcg caagacaggt
     1741 tcgaggggcc ggtccggggc gggatcgaaa ccaggggcgt cggcgacaca ggccccggcc
     1801 gcgtcacagg atgcgcatgt ggcgaacggg cgcgcggtga tgtcgatggc cagccgatca
     1861 agttcgggat agaggtccgc agtcgcaccc cggatgtcga gaacgcacgg tggagcgcct
     1921 tcgcggccac cagtcctgac gggaaccccg gcggtcctgg caagttccac tgcctcgatt
     1981 acctggccgg ccgcgagtcg atagtcccaa ccccgggggc cgcacc
//
LOCUS       DAOPPA010000532         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60200_length_2025_cov_3.866497, whole genome shotgun sequence.
ACCESSION   DAOPPA010000532 DAOPPA010000000
VERSION     DAOPPA010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60200_length_2025_cov_3.866497"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKH54_12845"
     CDS             complement(<1..626)
                     /locus_tag="PKH54_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOD00827.1"
                     /translation="MAATGESSNIDLVWWVAPISSLIALVMAFVLFRMMRKSPEGNER
                     MREIAQYVREGAFAYLRRQYKVVTIVFAVMVVLLGFLAFMGIQNPFVPIAFLTGGFFS
                     GLCGFIGMNTATLASNRTAEGARHSLNRGLQVAFRSGAVMGLVVVGFGLLDICLWYLI
                     LDKLVYTPENMKTGLQALGLIFVHPGTGEHDKLVEITVTMLTFGMGAST"
     gene            complement(909..>2025)
                     /locus_tag="PKH54_12850"
     CDS             complement(909..>2025)
                     /locus_tag="PKH54_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00828.1"
                     /translation="IPMFGYENDWAQTDGASSIDMTEYGEYVGHVAGRYCQNINIFEI
                     WNEENIPRFWSGKPDADAYGRMLKAAHQAITGACPEARVAFGGIASYDAETDLADRYG
                     FLKRVAAAHPDICDYFEVVAFHPYTFMQYDPPERDVIIDDTLAFQGQSWQTAILRDIL
                     KEIGCPDKSVIITEQGWPSYDLTEQQVGLFLPRSILLAIRDGVEKYLWYTFWDGYPTT
                     EGVRPHESYFGLFGWTGGDGTVRRAKPAWTALKALADNLGEMKFARDVSTALELPNDV
                     YALAFMDDRGRLTVAAWDGRDFPDTSWGVDGPGGEDTSHELLLPLPELYDGLAIYDIE
                     GNLLTEGIDYQVTYGETTTVTILLTPSVVYIRAFVLV"
BASE COUNT          413 a          595 c          608 g          409 t
ORIGIN      
        1 gtcgatgcgc ccatgccgaa cgtaagcatg gtgacggtga tttcgaccag cttgtcgtgt
       61 tcgccggttc cggggtgaac gaagatcagc ccaagggcct ggagtccggt cttcatgttt
      121 tcgggcgtat agaccagctt gtccaggatc aggtaccaca ggcagatgtc cagaaggccg
      181 aagccgacga cgacaagacc catgaccgcg ccactgcgga aagcgacctg gaggccgcgg
      241 ttaagcgaat gacgggcgcc ttccgcggtg cggttggacg caagcgtcgc ggtgttcatg
      301 ccgatgaagc cgcacaggcc ggaaaagaaa ccgccggtca ggaaggcgat cgggacgaac
      361 gggttctgaa ttcccatgaa cgccaggaat ccaagcagca cgaccatgac ggcgaacacg
      421 atcgtcacca ccttgtactg gcggcgaagg tacgcgaacg cgccctcacg cacgtactgg
      481 gcaatttcgc gcatacgttc gttgccctcg ggtgacttcc tcatcatcct gaacagcacg
      541 aatgccatta caagggcgat cagcgaagag atcggcgcaa cccaccatac aaggtcgatg
      601 tttgaacttt caccggttgc ggccatggcc ggaaccgcga aaatcagcgc ggcaatcccg
      661 gtcaggacgg caagcccatg actggcggca tccttaagca gtcgattcat cacacgtctc
      721 cagattgggt tcttttcaaa atgacgttgc gtccaaagcc cggcgatttt caatcaaaac
      781 cgccaatttt tcaaggtttg cccaagtttg ccatgcaccg ccgccgctgg ctttggggaa
      841 gcggattttt gcttaaaatt cagattgttg ggtggctgtg tcgggatgtc cggtggactg
      901 cgcctgaatc agaccagcac gaaagccctg atatagacga cggaaggcgt cagcagtatg
      961 gttaccgtcg tcgtttcgcc atacgtcacc tgatagtcga tgccctcggt cagcaggttg
     1021 ccttcaatgt cgtaaatcgc caggccgtcg taaagttccg gcaatggcag cagcagttcg
     1081 tggctggtgt cttcgccacc ggggccatcg acgccccatg aagtgtcggg gaagtcacgg
     1141 ccgtcccatg cggccaccgt caggcgtccc cggtcgtcca tgaaagcaag cgcatatacg
     1201 tcgttcggca gttcgagcgc ggtcgagacg tcgcgggcga acttcatctc gccaaggttg
     1261 tcggcaagcg ccttcaacgc cgtccaggcc ggcttggccc tgcgcacggt cccgtccccg
     1321 ccggtccagc cgaagagccc gaagtatgat tcgtgcggtc gaactccttc cgtggtcgga
     1381 tatccgtccc agaatgtgta ccacaggtat ttttcgaccc cgtcacggat ggcaagcagg
     1441 atgctgcggg gcaggaaaag gcccacctgt tgctcggtca ggtcgtatga aggccagccc
     1501 tgttccgtga tgatgaccga cttgtccgga cacccgattt ctttaaggat gtcgcgaagt
     1561 atcgcggtct gccaggactg cccctggaag gccagggtat cgtcgatgat gacgtcgcgt
     1621 tccggcgggt cgtactgcat gaacgtgtat gggtggaacg cgaccacctc gaagtaatcg
     1681 cagatgtcgg gatgggccgc ggcgacccgc ttcaggaagc catagcggtc ggcaaggtcg
     1741 gtttcggcgt cgtacgaagc aatgccgccg aacgccacgc gggcttcggg gcacgcgccc
     1801 gtgatggcct ggtgcgccgc cttcagcatt cgcccgtaag catcggcatc cggttttccc
     1861 gaccagaacc gcggaatgtt ttcctcgttc cagatctcga agatgttgat gttctggcaa
     1921 taccggcccg cgacatgacc gacgtattcg ccgtattccg tcatatcgat tgaactggcg
     1981 ccgtcggtct gtgcccagtc gttttcgtac ccgaacatcg ggatc
//
LOCUS       DAOPPA010000533         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60264_length_2024_cov_2.533773, whole genome shotgun sequence.
ACCESSION   DAOPPA010000533 DAOPPA010000000
VERSION     DAOPPA010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60264_length_2024_cov_2.533773"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            684..>2024
                     /locus_tag="PKH54_12855"
     CDS             684..>2024
                     /locus_tag="PKH54_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOD00829.1"
                     /translation="MKKLLTILSAGALAFAVACGGDTEKTDEGNPDVDLQDVANPDVE
                     EDVVTPDVGTDRVEEQVEPDVIDIVEPDTTDVVEEVVEMEDRHFTFRTIGGMSMGAAA
                     LTVASHYPEMFDSVGGMGGYVDYRYIGHLLKDMMGGGFCPMEDILSVVEQDALNGTDE
                     INNRDNPMVDCYLPVEERHLRPYEIYMTYNRMNYSKQAGTTFHRGFYIDVIGAIIYAF
                     GNFLFYNPDHPLAPAGVDPDWFLQSDSQKCANPMRVTFPNNLNAEYNPTGEYDLISFC
                     DGSPDVCRGIPDCDDESPEMRATRGWFYPDQPDRHNMPVAGFLAVDYNGNGKRDLGEP
                     VVFNLGERWDDVGVDGCANEFENGSGGCNENASGSADVDANGDDWDLMTNNTGTEGNS
                     EYDEGEPFDDFGIDGVDSTALVLKKAMPVVEVKDLGEGDGEYTYNPRWGALIEND"
BASE COUNT          468 a          580 c          572 g          404 t
ORIGIN      
        1 agtgcactca gggttcatcg gaaggccaat tgacctggca gaaatctgac tgcgacgaaa
       61 tcctgacgcc aatgggtcag gaatgtgggt tccccttgat cataaacaat tgcatccaaa
      121 ccgataaggt ggccacttgc tgtccctgga ctgcctggac atacagtgca ggactgggag
      181 ttccgtgttc gcggaatgag attggccaaa acagatgcgg tcaggggaat tacaccaagg
      241 ggggcgtgtt gcgtacatgc aatttcctaa gccaagctca gacgatttat ccgaacgatc
      301 cagcggaccc aaattctggc ggacccagtt cggacaaatg cgattatata tttgccgagt
      361 gtccctactg gtattactcc gaattgtgcc caaagctgga acgttgctat caccccacgg
      421 cctgcgagtc agggacgaat gaatatgggg aacccactaa cgtctgcgcc agggcttgca
      481 tctacgacac agaccaaggc gttgcccggt gcgccaagac ctgcgacgac cttttcaagt
      541 actacctcga ttgagttgga tcccgacccc actttttgcc ccaaaaatca cgataattgc
      601 tggaaatcgt gcccccttgg gggtataaat tcgcagaccc ggtatcgggc cccttcttgt
      661 aagcgttttt ttgggaggta accgtgaaga aactgctgac gatcctgtcc gccggcgccc
      721 tggcgtttgc cgtggcctgt ggcggtgata ccgaaaagac cgacgaaggc aatcctgacg
      781 ttgatttgca ggatgttgcc aaccccgacg ttgaagagga tgtcgtcacc ccggacgtgg
      841 gcaccgaccg cgtcgaggaa caggttgaac ccgacgtcat cgacatcgtc gagccggata
      901 ccaccgacgt cgtcgaagaa gtcgtcgaga tggaagaccg ccacttcacg ttcaggacca
      961 tcggcggcat gtccatgggc gccgctgccc tgaccgtggc ctcgcactat cccgaaatgt
     1021 tcgacagtgt cggcgggatg gggggatacg tcgattaccg gtacatcgga catctgctga
     1081 aggacatgat gggcggcggt ttctgcccga tggaagacat cctttccgtc gtcgagcagg
     1141 atgccctgaa cggcaccgac gaaatcaata atcgggacaa tccgatggtt gactgctatc
     1201 tgccggtcga ggaacgtcac ctgcgtccgt acgaaatcta tatgacgtac aacaggatga
     1261 attactcgaa gcaggcggga acgaccttcc atcgcggctt ctacatcgac gtcatcggcg
     1321 ccatcattta cgcattcggc aacttcctgt tttacaaccc ggatcacccg ctggctccgg
     1381 ccggagtcga tccggactgg ttccttcaga gcgattccca gaagtgcgcc aatccgatgc
     1441 gggtcacctt cccgaacaac ctgaatgccg agtacaaccc gaccggcgaa tacgacctga
     1501 tttcattctg cgacggctcg ccggacgtct gccgcggcat tcctgactgt gacgatgaat
     1561 cccccgagat gcgcgccacc cgcggctggt tctatccgga tcagcccgac cgccataaca
     1621 tgcccgtcgc gggtttcctt gctgttgact acaacggcaa cggcaagcgc gaccttggcg
     1681 agccggtcgt gttcaacctg ggtgaacgct gggatgacgt tggcgtcgat ggctgcgcca
     1741 acgaattcga aaacggcagc ggcggatgca acgaaaacgc atccggatcg gccgacgtcg
     1801 atgccaacgg cgacgactgg gacctgatga cgaacaacac tggaaccgaa ggcaacagcg
     1861 aatacgacga aggcgaacct tttgacgact tcggcatcga tggcgtcgat tccaccgcac
     1921 tggtactgaa aaaggccatg ccggttgtcg aagtcaagga tcttggcgaa ggcgacggcg
     1981 agtacactta caatccgcgc tggggcgccc tgatcgaaaa cgac
//
LOCUS       DAOPPA010000534         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60359_length_2021_cov_4.635809, whole genome shotgun sequence.
ACCESSION   DAOPPA010000534 DAOPPA010000000
VERSION     DAOPPA010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60359_length_2021_cov_4.635809"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2021)
                     /locus_tag="PKH54_12860"
     CDS             complement(<1..>2021)
                     /locus_tag="PKH54_12860"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOD00830.1"
                     /translation="MALVDSVLPTFALGLDVTTDYILGPDDNFVEIRTTIKDPNNSGM
                     TFDVGQFLQFGNRATTFFDRCGVDPDCLAGKKNVRWLASAAGDVSYALTVPGTDFVSL
                     LLAQSELLILASGQFEIPKNGEIVTRSFLIVDKGTIDDVARTARRIRGEAEGIEVTIN
                     VTLSDTFSDMKDVFIKAKIAEESNSVGWRSATAPDAEGKAVISLEPGVYDITVSLPGA
                     PDALLDDVTVSADGLNEFDVTMAAAGRVHVAVADGDDQPINAAITFQNGADAPWTNGV
                     ARYEAVHNGDRTFPILPGTYTATIGRGLAYTIDRKNVTVTAGEITDIVGSIDAAYDVS
                     GFVMMNSHEHSEHSIDSAVLVEDRVWNALANGIQVMNPTDHDFFGTRMDTIQNLDVQE
                     LVTSALSCEVSPIWGHTTAADCSNPPPYPTYFAVNYTLYNESGAAERAMTATEIYNQV
                     RNELQCNFLAINHPYRGGPTFETYGIGADTDPKIALPDLDINLVNALEVINKDDSLDS
                     ILNENLPAWFNLLNRGYVVAGIGGSDEHHYRGNYGNPRNAVMLDATDMSQVDVSDVFN
                     NVKGFKNLVMSGPMVRLTVDGYGMGDVVTMAGETVEVHLLIEAPEWMGLNFCKVFMNG
                     ELIREFTPVVDGAVLRLDETFTVTPESDAWIVAYAGSNLPEHEMTPVAD"
BASE COUNT          357 a          575 c          635 g          454 t
ORIGIN      
        1 ttgtcggcga ccggggtcat ctcgtgctcc ggaaggttcg agccggcgta cgcgacgatc
       61 catgcgtcgg attccggggt gacggtgaat gtttcgtcca ggcgaagcac ggcgccgtca
      121 acgacgggag tgaactccct gatcaattcg ccgttcatga agaccttgca gaagttcagg
      181 cccatccatt ccggtgcttc gatgagcagg tggacttcaa cggtctcccc ggccatcgtt
      241 acaacgtcgc ccattccgta tccgtcaact gtcaggcgaa ccatcgggcc gctcatgacc
      301 aggttcttga aacccttcac gttattgaat acgtcgctga catcgacctg ggacatgtcg
      361 gtcgcgtcaa gcatgaccgc gttgcgcggg ttgccgtagt tgccgcggta gtggtgttcg
      421 tccgaaccgc cgatgccggc cacgacataa cctcgattga gcaggttgaa ccatgccggc
      481 aggttttcgt tcaggatgct gtccagcgag tcgtccttgt tgatgacttc cagggcattg
      541 acaaggttga tatccagatc cggaagcgcg atcttcgggt cggtgtcagc cccgattcca
      601 taggtttcaa aagtcgggcc gcccctgtag ggatggttga ttgccaggaa gttgcactgc
      661 agttcgttcc tgacctggtt atagatctcc gtggcggtca ttgcgcgctc ggcagcgccg
      721 gattcgttgt aaagcgtgta attaacggcg aagtacgtcg ggtacggcgg cggattcgag
      781 cagtcggccg cggtcgtgtg gccccagatt ggactgacct cgcaactgag tgcgctggtg
      841 acaagctcct gaacgtcaag gttctggatc gtgtccatcc tggttccgaa gaagtcgtga
      901 tccgtcgggt tcattacctg aatgccgttt gcaagggcgt tccagacgcg atcctcgaca
      961 agaacggcgc tgtcgatgct gtgctcgctg tgctcgtggc tgttcatcat cacgaatccg
     1021 gacacgtcgt acgcggcatc gattgaccca acgatatcgg tgatttcgcc ggccgtcacg
     1081 gtgacgttct tccggtcaat cgtgtatgcc aggccgcggc cgattgttgc cgtgtaagtt
     1141 ccgggaagta tcgggaatgt cctgtccccg ttgtggacag cctcgtacct ggccactccg
     1201 ttcgtccacg gtgcgtcggc gccgttctgg aatgtgatgg cggcgttgat gggctggtcg
     1261 tcgccatcgg cgacagccac gtgaacgcgg ccggccgcgg ccatcgtgac gtcgaactca
     1321 ttaaggccgt cggcgcttac cgttacgtcg tccaggagcg cgtccggcgc gcccgggagc
     1381 gacacggtga tgtcatagac gccgggttcc aggctgatga ccgccttgcc ttcggcgtcc
     1441 ggggcggtgg ccgagcgcca tccgacggaa ttgctttcct cggcgatttt tgccttgatg
     1501 aagacgtcct tcatgtcgga gaaggtgtcg gaaagggtga cgttgatcgt cacttcgatg
     1561 ccttcggcct ctccgcggat gcgtcgcgcg gttctggcga cgtcgtcgat ggttcccttg
     1621 tcaacgatca ggaagctgcg agtgacgatt tcgccgttct tcgggatttc gaactgtccg
     1681 gaagccagga tcagaagctc gctctgggca agcagcaggc tgacgaagtc ggttccagga
     1741 acggtcagcg cgtaggacac gtcgccggcg gcagacgcca gccacctgac gttcttcttt
     1801 ccggccaggc agtcgggatc gacgccgcag cgatcgaaga acgtcgtggc gcgattgccg
     1861 aactgaagga actgcccgac gtcgaatgtc attccggagt tgttcggatc cttgatcgtc
     1921 gtcctgattt cgacgaagtt gtcgtccgga cccagaatgt agtcggtcgt gacgtccagt
     1981 cccagggcaa atgtcggcag aaccgaatcg accagggcca t
//
LOCUS       DAOPPA010000535         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60398_length_2020_cov_5.179644, whole genome shotgun sequence.
ACCESSION   DAOPPA010000535 DAOPPA010000000
VERSION     DAOPPA010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60398_length_2020_cov_5.179644"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="dnaB"
                     /locus_tag="PKH54_12865"
     CDS             complement(<1..1316)
                     /gene="dnaB"
                     /locus_tag="PKH54_12865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOD00831.1"
                     /translation="MIPGDKPPPHSPEVEAALIGAALVNQQAFPLIAEQVAAEEFYDS
                     RHQRIFEAMSKLFTATVVIDPVTVANSLKESRNLEAAGGTEYLAALVSLSVPLDGCEH
                     YARIVRTSAALRRMIFTAAEITAEAYNGGYEPEEFLSMAEEKLLKAGEKRISKQVVRV
                     GEMFPEALRQIEIRRANHTMAVGISSGYKSLDGLLTGFHNSDLIILAARPAMGKTSFA
                     LNLALNAAFNQNKAVLFVSLEMGMSQIVDRILCVKTGVDSKRLRTAQFLAAPDLNLIR
                     KATVDMDQTPLYIDDTAKIGMLELRAKARRLKAMGQLDMIMVDYLQLMDPSDKRVSRE
                     QQISEISRNLKAMAKEMNIPVMALSQLSRAPETRPGDKRPMLSDLRESGAIEQDADVV
                     MFLYRPEYYKRQIKSEKSVRRGKPEETDVATEDQNITNLLEVMVEK"
     gene            1484..>2020
                     /gene="mutL"
                     /locus_tag="PKH54_12870"
     CDS             1484..>2020
                     /gene="mutL"
                     /locus_tag="PKH54_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOD00832.1"
                     /translation="MPDRFVQVLPDFLINQIAAGEVVENPASVVKELVENSIDAGARR
                     VAVEIEAGGLGLVRVIDDGCGMSPQDALAAMGRHATSKIRSMEDLMSIATLGFRGEAL
                     PSIASVSRFTMRTRLSGDDAGTQVEAVDGQVVSRPCACPVGTCIEVRDLFYNVPARLK
                     FQKAESARSMAVADTVRKA"
BASE COUNT          355 a          581 c          631 g          453 t
ORIGIN      
        1 tgtttttcaa ccatcacttc cagcaggttt gtgatgttct gatcctcggt ggcgacatcg
       61 gtttcttccg gcttgccacg tcttaccgat ttttcgcttt ttatctggcg cttgtagtac
      121 tcgggacggt aaaggaacat gacgacgtcg gcgtcctgct cgattgcgcc ggattcgcgc
      181 aggtccgaca gcatcggccg cttgtccccc ggtcgtgttt ccggggcgcg ggaaagctgc
      241 gacagcgcca tgacggggat gttcatttcc ttcgccatgg ccttcaggtt acggctgatt
      301 tccgaaatct gctgttcacg gctgacccgc ttgtccgacg gatccatcag ttgaaggtaa
      361 tcgaccatga tcatgtcaag ctggcccatc gccttcaggc gccgggcctt tgcccgaagt
      421 tccagcattc cgattttcgc ggtgtcgtcg atataaagcg gcgtctgatc catatcgacg
      481 gtcgccttgc ggatcaggtt caggtcgggc gcggccagga actgggccgt ccgcaggcgc
      541 ttggaatcga cgccggtctt gacgcacagg attcggtcaa cgatctggct catgcccatt
      601 tccagggaca cgaacaggac agccttgttc tggttgaaag cggcgttaag cgccaggttc
      661 agggcgaagg acgtcttgcc catggcggga cgcgcggcca ggatgattaa gtccgaattg
      721 tggaatccgg tcagcaggcc gtccagggat ttgtaaccgg acgaaattcc gacggccatg
      781 gtgtggttgg cccgcctgat ttcgatttga cgaagagcct cgggaaacat ctcgccgacg
      841 cgcacaacct gcttcgatat gcgcttctcg cccgctttca gcagcttttc ctcggccatg
      901 gacaggaatt cttccggttc gtagccgccg ttatacgcct cggcagtaat ttcggccgcg
      961 gtgaaaatca ttcggcgcag tgccgcggaa gtcctgacaa tccttgcgta gtgttcgcat
     1021 ccgtccagtg gaaccgacag ggaaaccagg gcggccaggt actcggtgcc gccggcggcc
     1081 tcgaggttgc gggattcctt caggctgttt gccacggtca ccgggtcgat caccacggtt
     1141 gccgtaaaca gcttcgacat ggcctcgaag atgcgctggt gcctggagtc gtaaaattcc
     1201 tcggcagcca cctgctcggc aatcagtgga aatgcctgct ggttgaccag cgcggcaccg
     1261 ataagcgcgg cctcgacttc gggcgaatgg ggtggcggct tgtctcctgg aatcacggta
     1321 tctcctgttc cgggcgtggt cgaagatttc gccgaatcat acatcgaaag ggtgccggtt
     1381 gtgcgtgcga aatgtcatcc accctaccgg gcgcggcacc gcaggcggtt gacgtccgtg
     1441 tcgcattgcc ataatttcat cgggcccgat tgagtggtga cagatgcccg acagattcgt
     1501 gcaggtactt cccgatttcc tgattaacca gattgcggcc ggcgaggtcg tggaaaatcc
     1561 cgcgtcggtt gtaaaggagc tggttgaaaa ttcgatcgac gcgggcgccc gccgtgtggc
     1621 cgtggagatt gaggccggcg gcctggggct tgtccgggtg atcgacgacg gatgcgggat
     1681 gtcgccacag gacgccctgg ccgccatggg ccgccatgcg acgtcgaaga ttcgttcaat
     1741 ggaagacctg atgtcgattg cgacgcttgg cttccgtggc gaggcccttc catccatcgc
     1801 gtcggtttcc aggttcacga tgcgtaccag gctttcgggg gacgatgccg gcacccaggt
     1861 tgaagcagtc gacggccagg tcgtttcgcg cccgtgcgcg tgtcccgtcg gcacctgtat
     1921 cgaggtcagg gatctttttt acaacgttcc ggcccgactg aagttccaga aggccgaatc
     1981 cgcccgctcg atggcggtgg ccgacacggt caggaaggcc
//
LOCUS       DAOPPA010000536         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60447_length_2019_cov_3.037169, whole genome shotgun sequence.
ACCESSION   DAOPPA010000536 DAOPPA010000000
VERSION     DAOPPA010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60447_length_2019_cov_3.037169"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKH54_12875"
     CDS             <1..268
                     /locus_tag="PKH54_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD00833.1"
                     /translation="REALYEPVSAIANAVRQALEGTPPELSADIVDKGIMLAGGGALL
                     KNIDVLIREETGLPVMIAEDPVASVVLGAGRILDDMDLLRRSAI"
     gene            296..1180
                     /gene="mreC"
                     /locus_tag="PKH54_12880"
     CDS             296..1180
                     /gene="mreC"
                     /locus_tag="PKH54_12880"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD00834.1"
                     /translation="MRDWLFQHRFTAFAAVLVILPLLLMYVTGSTIGSSGPIENGTAA
                     MTGWAQTGAGSVVNSVGSFSSRIGAVFSVASENFRLSQENQRLLGTSLKARRIEAENR
                     ELRLLLGLRESRPELKLLPAEVVLRDVNPFFRVERVRLLMAEKREDIPAASAGNAVIT
                     SAGLVGRVTEIRRSFADVMLVSDHRSRVAVQVHGTGIIGMVVGSGRKAGFQARVQVPV
                     SEQALQKDAVIVTSGHDRVFPRGIEVGYIADPEARRQVGPFMEYDIVLAEDPSTVDTV
                     MVVTELPVAESIDAFGGK"
     gene            1180..1695
                     /locus_tag="PKH54_12885"
     CDS             1180..1695
                     /locus_tag="PKH54_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00835.1"
                     /translation="MRNVFVLIAVFVCMMIQVPILTLLGLTTFSVDAPLVAMLYIAST
                     GPGVVGMVIVSLMGLLTDLFMPGGLPGMQMEILVVLFLLARMVVSRLQLNRTLPLIIV
                     VFLSSLVSLGLFMLLSVIFDRGYQHSSTVVTGGLAQALLTALLSPLLFKLFGVADGRF
                     HHRRDTRSLFV"
     gene            1744..>2019
                     /locus_tag="PKH54_12890"
     CDS             1744..>2019
                     /locus_tag="PKH54_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD00836.1"
                     /translation="MIGLQDGLVEYRTHFRWAVILICALLLVLVGRLFHLQIVKGRMY
                     ETLATVSHVVRERTIPPRGSIFDRNGKLLATDVEVSDLVAIPHNVTDP"
BASE COUNT          322 a          602 c          616 g          479 t
ORIGIN      
        1 acgcgaggcg ctttacgagc cggtttccgc aatcgcgaac gccgtcaggc aggcgcttga
       61 aggcactccg cccgaacttt cggccgacat tgtcgataag ggcatcatgc tggccggcgg
      121 cggcgccctg ctgaagaaca ttgacgtcct gatccgcgag gaaaccgggc tgcccgtgat
      181 gatcgcggaa gacccggtcg cgtccgtcgt gcttggcgcc ggacgcatcc ttgacgacat
      241 ggacctgctg cgtcgcagtg caatctgaca tctgcgggcc cgtggtggca tgattatgcg
      301 tgactggctg tttcagcaca gatttacggc attcgcggca gtccttgtga tccttccact
      361 gctgctgatg tacgtgaccg gcagcaccat cggttcttcg ggcccgatcg agaacgggac
      421 ggccgcgatg accggatggg cccagaccgg cgccggcagc gtcgtcaatt cggtcggttc
      481 gttttcgtcc cgcatcggcg ccgtcttttc cgttgccagc gaaaatttca ggctcagcca
      541 ggaaaatcag cgtcttcttg gtacttcgct gaaggctcgt cgaatcgagg cggaaaaccg
      601 tgagttgcgc cttcttctgg ggcttcgcga aagccgtccg gaattgaaac tgttgccggc
      661 cgaggtcgtc ctgcgggacg tcaatccgtt cttcagggtt gaacgcgtca ggctcctgat
      721 ggccgaaaag cgggaagaca ttccggccgc gtccgccggg aacgcggtga tcacgtccgc
      781 cgggctggtc gggcgagtca ccgagatccg ccgatccttc gcggatgtca tgctggtctc
      841 ggatcaccga agccgggtcg ccgtccaggt tcatggcacg gggatcatcg gaatggttgt
      901 cggttcggga cgcaaggccg gtttccaggc cagggttcag gttcccgttt ccgagcaggc
      961 cctgcagaag gacgcggtaa tcgtcacttc cgggcatgat cgggtgttcc cacgcgggat
     1021 cgaggtcggt tacatcgccg accctgaagc ccgccgtcag gtcggtccgt tcatggaata
     1081 cgacatcgtc cttgccgagg atccttcgac cgtcgatacc gtgatggtcg tcacggaact
     1141 gccggtggcc gagtccatcg acgccttcgg gggtaagtga tgcgtaacgt gtttgtgctg
     1201 attgcggttt tcgtctgcat gatgatccag gttccgatcc tgaccctgct cggcctgacg
     1261 actttttccg tcgatgcgcc ccttgtggcc atgctttaca tcgcatcgac cgggccgggc
     1321 gtcgtcggca tggtgatcgt ctcgctgatg ggcctgctta cggatctgtt catgcccggc
     1381 ggtcttcccg ggatgcagat ggaaatcctt gtggtgctgt tcctgctggc ccgcatggtc
     1441 gtgtcccgtc tgcagttgaa ccgaaccctt ccgctgataa tcgttgtatt cctgtccagc
     1501 ctggtgtcgc tgggcctttt catgctgctt tcggtcatct tcgaccgcgg ttatcagcac
     1561 tcatccacgg tggtgacggg cggtctggcc caagccctgc tgaccgcgct tctttcgccg
     1621 ctgctgttca aacttttcgg ggtcgccgac ggacgattcc atcacaggcg tgacacccgc
     1681 agtcttttcg tgtgatcatt cgcctgcccg gagggtggct tttccctttt acggagcgtt
     1741 accttgatcg gtcttcagga cggccttgtt gagtacagga cccatttcag gtgggccgta
     1801 atcctcatct gcgcccttct gctggttctg gtcggaaggt tgttccacct tcaaatcgtc
     1861 aagggccgga tgtacgaaac cctggccacc gtcagtcatg tggttcgtga gcgcactatt
     1921 ccgccacgtg gaagcatctt tgatcgtaat ggcaaattgc ttgccaccga tgtcgaggtc
     1981 tcggatcttg tcgccattcc gcataacgtg accgaccca
//
LOCUS       DAOPPA010000537         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60589_length_2015_cov_4.280612, whole genome shotgun sequence.
ACCESSION   DAOPPA010000537 DAOPPA010000000
VERSION     DAOPPA010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60589_length_2015_cov_4.280612"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PKH54_12895"
     CDS             <1..1630
                     /locus_tag="PKH54_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00837.1"
                     /translation="NQPAGDLMVHAEITNINDAVNGNPANGDAFIENPNLLTNSTDGI
                     ARIGFNAGTLEGVIYEVTVSIPEFPDVASKTILMRVFDIPCGCLRTSMSYDGTIEMLR
                     DIEVYILPEQYRCNSLSATREVPEAAIIASRTASSMYSGITFDCLPGEKRYTIFTKAG
                     GTNSTCVAATGCYEGMYISPNVCQDLELDLYSTVLNPAGAYDSIDHFDFRNMVKQCAG
                     GETSIMTCVAGGTDIGKTVCCALQEMIKFFETPGTTIMEGIRDIVKLWVGAIIVDTVY
                     NLIGNIIADWITGWLRNSSPDWLQDFFDIGEDMMGTITNLELVSDLLIKKLNNDQTVQ
                     GTHYWYGMHLYWKFGCDPDAPDYDQCGLIDLNLAEYIDDPAFPLNFLEGKFTAAISDF
                     DKMYLNQHEIKLNYGKLVLYVINEVVIAGLTKNSPEGPAHSMLELVFRWIDCAGFAAG
                     TLGDILEAIGIGSQQDLEQACRTTLTGLFGFVEAFLNALALDTTLSLTGSARMMDKDC
                     DLMVDQIINGVYTGYIQGDTSQALVTGDFEATRK"
     gene            1642..>2015
                     /locus_tag="PKH54_12900"
     CDS             1642..>2015
                     /locus_tag="PKH54_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00838.1"
                     /translation="MKKLPFRPVAPTVLLVMLIAWGCGYKREIMPLVGEDDKALEFSG
                     QSRWYLDKSAASMAFEEFARAVQAGDAEGCVAALGPMTLALLNGQAAAKGMDAVRYWR
                     TGDISMIVLPGTGKPVSMLKNQ"
BASE COUNT          462 a          581 c          585 g          387 t
ORIGIN      
        1 gaaccagccg gccggcgacc tgatggtcca cgcggaaatc accaacatca acgacgccgt
       61 caacggcaat ccggcaaacg gcgatgcgtt cattgaaaac cctaacctgc tgacgaactc
      121 gacagatggc atcgcaagga tcggattcaa cgcgggcacg ctcgaaggcg tcatttacga
      181 agtcaccgtt tccataccgg agtttcccga cgtcgcctcc aagaccatcc tgatgcgcgt
      241 gtttgacatt ccgtgcggct gcctcaggac ttccatgtcg tatgacggca ccatcgagat
      301 gctgcgcgac atcgaagtct atatcctgcc ggagcagtac cgatgcaatt cgctgtcggc
      361 aaccagggaa gttcccgaag cggccatcat cgcctcgcga accgcgtcca gcatgtacag
      421 cggcatcacg ttcgactgcc tgccgggtga aaagcgctac acaatcttca ccaaggcagg
      481 tggaaccaac tcgacgtgcg ttgcggcaac cggctgctac gaagggatgt acatcagccc
      541 gaacgtttgc caggatctgg aactggatct gtactcgacc gtgctgaacc cggccggggc
      601 atacgactcg attgaccact tcgacttcag aaacatggtc aagcagtgtg ccggcggcga
      661 aacttcgatc atgacctgcg tggccggcgg aaccgacatc ggaaagaccg tgtgctgcgc
      721 cctgcaggaa atgatcaagt ttttcgagac tcccggaacg accatcatgg aaggcatccg
      781 ggacatcgtg aagctctggg tcggggcgat tatcgtcgat acggtttaca acctgatcgg
      841 gaacatcatc gccgactgga tcaccggatg gctgcgcaac agttcgccgg actggctgca
      901 ggatttcttc gacatcggcg aagacatgat gggcaccatc accaacctgg aactggtgtc
      961 cgacctgctc atcaagaaac tgaacaacga ccagaccgtt cagggcaccc actactggta
     1021 cggaatgcat ctctactgga aattcggatg cgatccggac gcacccgact acgaccagtg
     1081 cggcctgatc gaccttaacc tggccgaata catcgatgac ccggctttcc cgctgaattt
     1141 cctggaaggc aaattcacgg cggcgatttc cgacttcgac aagatgtacc tgaaccagca
     1201 cgagatcaaa ttgaattacg gcaagctggt tctgtatgta atcaacgaag tggtcattgc
     1261 gggattgacc aagaacagcc cggaagggcc agcccacagc atgctcgaac tcgtgttcag
     1321 atggatcgac tgcgccggat tcgccgcggg aaccctgggt gacatacttg aggcaatcgg
     1381 gattggcagc cagcaggatc tggaacaggc ctgccgaacc accctgaccg gccttttcgg
     1441 attcgtcgag gcgttcctga acgccctggc cctggacacc acgctttccc tgaccggcag
     1501 cgccaggatg atggacaagg actgcgacct gatggttgac cagatcatca atggcgtgta
     1561 taccgggtac atccagggcg atacgtcgca ggcactggtt accggcgact ttgaagcgac
     1621 ccgcaagtaa gacttaacct gatgaagaaa cttcctttcc gaccggttgc accaactgtc
     1681 ctgctggtca tgctgatcgc gtggggctgc ggatacaagc gtgaaatcat gcccctggtg
     1741 ggcgaggacg acaaggcgct ggaattttcc ggacagagca gatggtacct ggacaagtct
     1801 gccgcctcga tggccttcga ggagttcgcg cgcgcggttc aggccggcga tgctgaaggc
     1861 tgcgtggcgg cgctcggtcc gatgacgctt gccctgctga acggacaggc ggcggcgaag
     1921 gggatggatg cggttcggta ctggcgtacc ggcgacatat cgatgattgt gcttcccgga
     1981 acgggaaagc cggtctcgat gctgaagaat cagtt
//
LOCUS       DAOPPA010000538         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_60813_length_2009_cov_3.349028, whole genome shotgun sequence.
ACCESSION   DAOPPA010000538 DAOPPA010000000
VERSION     DAOPPA010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60813_length_2009_cov_3.349028"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKH54_12905"
     CDS             complement(<1..427)
                     /locus_tag="PKH54_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00839.1"
                     /translation="MRQFCTVFAVCLVSCLISASSAGQTLTDTWNSQVVYKPVEQANK
                     AAPAPAPAPTPAAQQTRPAEPPKPAPKPAQVDATGPMRFGIALVQGLKAFAEPGVEMK
                     GTGWVFPILHPMKIVESVCVARDGSRLEPNREKVDGTVKE"
     gene            complement(489..1766)
                     /locus_tag="PKH54_12910"
     CDS             complement(489..1766)
                     /locus_tag="PKH54_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00840.1"
                     /translation="MKKVVFSAVDSRMAYSSLGYGAVFSGMQPCDHAWLDGSGADARP
                     FVVESGASLWSYDVIFVSIAWELELPSLVKALRSSGIEPFAARRPASQPLLVGGGPLT
                     LSNPNLPGAVCDAVFIGEADGAFADLNRALHVATGREDLLSRLAAIPGMWVPSSGAAC
                     PDPVVAKLPEIPLFSAFPGRDNMFSGSFIVEVGRGCPRGCAFCVAFGGRRARFYPSEA
                     ITAQVPDGTSRVGLLGAAVSDHPDLKGIVSTLRDRGIGVTLGSLRADRVEPDLVSMLA
                     AGGLRTLTVAADGASESLRKSIHKGVTADHLLEAARVARETGIEKVRVYVMLGLPGEQ
                     SGDVAEFAGLVNAMAAITNVVVSVSPFVPKRFTPLSDAGFAGVGTIRRRISELKRMVR
                     PAVQIRAGSVKEAEFEHRLSHAEMREIPDLTLS"
     gene            complement(1798..>2009)
                     /locus_tag="PKH54_12915"
     CDS             complement(1798..>2009)
                     /locus_tag="PKH54_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00841.1"
                     /translation="TDSPRVALFQNAIPSPIFELDPRGISCIRQSQKVRAELRRSVGV
                     GDLIVGRQLRQHEFFRNLVTSKLQS"
BASE COUNT          457 a          609 c          606 g          337 t
ORIGIN      
        1 tttccttgac ggttccatcg accttttcgc ggttcggttc cagcctgctg ccatccctgg
       61 caacgcaaac gctttcaaca atcttcatcg gatgaagaat cgggaaaacc catccggtac
      121 ctttcatctc gacgccgggc tcggcaaatg ccttcaagcc ctgaaccagc gcgataccaa
      181 agcgcatcgg gccggtggca tcgacctggg cgggcttggg ggccggcttc ggcggctcgg
      241 ccggcctggt ctgctgggca gccggcgtgg gtgcgggggc cggggccggg gcagccttgt
      301 ttgcctgctc gaccggctta taaacaacct ggctgttcca tgtatccgta agcgtctgac
      361 cggcggaaga cgccgaaatc aggcaggaaa ccagacatac agcaaatacg gtacaaaact
      421 ggcgcatgat tgatacacaa cctccagata cagtgcggtt tttcaagctg gttgaagttt
      481 atccggtgtc aggaaagagt caagtctgga atttcgcgca tttccgcgtg cgaaagccgg
      541 tgttcgaact cggcttcctt gaccgaacct gccctgattt ggacggcggg ccgcaccatg
      601 cgcttcagtt cggaaatccg ccggcggatg gtgccaacac cggcaaagcc ggcatccgaa
      661 agtggtgtga agcgcttcgg aacgaacggc gaaaccgaaa caacgacatt tgtgattgcg
      721 gccatggcat tgaccaatcc ggcgaattcc gcgacgtcgc ccgactgttc cccgggcagc
      781 ccgagcatga catagacgcg cactttttcg atcccggttt ccctggcgac cctggctgct
      841 tccagcaggt ggtcggcggt gacgcccttg tggatcgact ttcgaagcga ttcggatgcc
      901 ccgtcggcgg caacggtcaa cgtgcgaagg ccaccggccg ccagcattga aaccaggtcc
      961 ggctcgacgc gatccgcccg aagggatcca agcgtgaccc cgatgccgcg atcccgaagg
     1021 gtggaaacaa tccccttcaa atcgggatga tcggaaaccg ccgcgcccag caggccgaca
     1081 cgggaagttc catcgggcac ctgggcggta atggcttcag acggatagaa ccttgcgcga
     1141 cggccaccga aggcgacaca gaaggcgcat ccgcggggac agccacggcc gacttccacg
     1201 atgaaggatc cggaaaacat attgtccctg cccgggaagg ccgaaaacag gggtatttcc
     1261 ggaagcttag ccaccacggg atcgggacaa gcggcaccgg acgacggaac ccacattcct
     1321 ggaatcgcgg caagacgcga aagaagatct tcacgaccgg ttgccacatg aagcgcacga
     1381 ttcagatcgg caaaggcgcc atcggcctcg ccgatgaaaa cggcatcgca cacggcgccg
     1441 ggcaggttgg gatttgacag ggtaagcggg ccgccaccga ccagaagagg ctggctggcg
     1501 ggacggcgcg ccgcaaaagg ctcgattccg gaagaccgca gggccttgac cagcgacgga
     1561 agctccagtt cccacgcgat gctgacgaag atgacgtcat acgaccacaa tgacgccccg
     1621 gactcgacca cgaacggcct ggcgtcggcc ccgctgccgt caagccacgc gtggtcgcac
     1681 ggctgcatgc cggaaaagac cgcgccgtaa cccagcgatg aatacgccat ccttgaatcg
     1741 actgcggaaa agacaacctt cttcaaatcg gaaccagttg aaatacccgg caagatgtta
     1801 cgactgaagc ttcgaggtga ccagatttcg gaagaactcg tgctgacgaa gctgccgccc
     1861 gacgattaag tccccgacgc caacggatct gcgcaattcg gcgcgcacct tctgcgactg
     1921 acggatacag gaaattccac gcggatccag ttcaaaaatc gggctgggga tcgcgttttg
     1981 aaaaagagcg acgcgcgggg aatcggttc
//
LOCUS       DAOPPA010000539         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_61412_length_1993_cov_2.862229, whole genome shotgun sequence.
ACCESSION   DAOPPA010000539 DAOPPA010000000
VERSION     DAOPPA010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61412_length_1993_cov_2.862229"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKH54_12920"
     CDS             complement(<1..314)
                     /locus_tag="PKH54_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD00842.1"
                     /translation="MSLLDITVEQALANRPGLGALRVAVEKEILHQDILRILGDAGFL
                     KGLVFMGGTCLRLCHGSNRLSEDLDFAGGRGFDRQAVVGLGRAITDGLSRKYGLAVTV
                     SEP"
     gene            complement(301..849)
                     /locus_tag="PKH54_12925"
     CDS             complement(301..849)
                     /locus_tag="PKH54_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00843.1"
                     /translation="MQPVRRLKQGLAKLATPERCLFTPSDMRALVPDISDQSWRALLS
                     RSAQEIGLVRLCRGLYLYLPAERPDGLLLFRAAARLRARDFYYLSLETVLSEAGVISQ
                     VQQCWISVMTSGRGGTISCGRRGTIEFIHTSRKPDQLAQHLTYDDRYGMLRADVTLAI
                     RDMRATRRDLDLIDWSVVNEPA"
     gene            complement(1030..1959)
                     /gene="argF"
                     /locus_tag="PKH54_12930"
     CDS             complement(1030..1959)
                     /gene="argF"
                     /locus_tag="PKH54_12930"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOD00844.1"
                     /translation="MKVRHLLAIKDLDREDLDEIFALAADMKANPERYGDALHNKTMA
                     MIFEKSSTRTRVSFECAMARMGGHALFLSPKDTQLGRGETVSDTAQVLGRMVDIIMAR
                     VFKHGDVVTLAEKSGVPVINGLSDYAHPCQALADYLTILEHKGKFEGLRVVYVGDSNN
                     NVTHSLMECGALLGVSVDCACPKGYYPAPEVVEWCREVGKKTGATFNVIEDPVEAVKG
                     ADVIYSDVWVSMGVEHEKEQRLIDLHDYQVNLALFEKAKSDCIFMHCLPAHRGEEVTD
                     DVCDHPRSVIFDEAENRLHAQAALILCLFAAWK"
BASE COUNT          407 a          618 c          580 g          388 t
ORIGIN      
        1 ggctcgctga ccgttacggc aagcccgtat tttcgggaaa gaccgtccgt tatggcccga
       61 ccaaggccga ccaccgcctg ccgatcaaac ccgcgccctc cagcgaaatc aaggtcttca
      121 ctgagcctgt ttgaaccgtg gcacagacgc aggcaggtgc cgcccatgaa tacgagcccc
      181 ttcaggaagc cggcgtcgcc cagaatccga agaatgtcct ggtgaaggat ttccttttcg
      241 accgccacgc gcagcgcccc gagtccaggc cggtttgcca gcgcctgttc cacggtgatg
      301 tcaagcaggc tcatttacga cactccagtc aataaggtcc agatcgcgac gggtcgcgcg
      361 catgtcgcgg attgccagcg tcacatcggc gcgcaacatg ccgtatcgat cgtcataggt
      421 caggtgttgg gcaagctggt ccggcttccg gctggtgtga atgaactcga ttgtcccgcg
      481 gcgcccgcac gaaatggttc cgccccgccc cgaagtcatg accgatatcc agcactgctg
      541 cacctgggaa atcacccctg cttccgagag aacagtttcg agcgacaggt agtagaaatc
      601 acgggcgcga agcctggccg ccgccctgaa caacagcagc ccatccggcc gctcggccgg
      661 caggtacagg tacaggcccc ggcaaagccg gacaagcccg atctcctggg ccgatcggct
      721 cagcagcgct cgccatgact ggtcgctgat atccggaacc agcgcccgca tgtccgacgg
      781 cgtgaacagg catctttccg gcgtggccag ctttgccagt ccctgcttca atctccgaac
      841 gggttgcata acgcactcaa caaaaagtta cgtaaagcgt aacttaacgt ttagttaaaa
      901 tcaaatctgg gtgcacgccc ctgactccag tgcggctttc aattcatcct tggggtctgg
      961 ccggtgacat taacgatgga agggatcacg ggcggcagta atccgcccct gcacggtgga
     1021 tgtggtcatt catttccacg cggcgaacag gcacagaatc agggcggcct gggcgtgcag
     1081 gcggttctcg gcttcgtcga agattacgct gcgcggatga tcgcagacgt cgtcggtgac
     1141 ttcctcgccg cggtgcgccg gcagacagtg catgaatatg cagtcggact tcgccttttc
     1201 gaacagggcc aggttgacct ggtaatcgtg caggtcgatc aggcgctgct ccttttcgtg
     1261 ctcgacgccc atcgaaaccc agacgtcgct gtaaatgaca tcggcgccct tgacggcctc
     1321 gaccggatcc tcgatcacgt tgaatgtggc gccggtcttc ttcccaactt cacggcacca
     1381 ctccacgacc tcgggggccg ggtagtagcc cttcgggcag gcgcaatcga cggacacgcc
     1441 aagcagggcg ccgcattcca tcaggctgtg cgtcacgttg ttgttggaat cgccgacata
     1501 gacgacgcgc aggccctcaa acttgccctt gtgttccagg atggtcaggt aatccgccag
     1561 cgcctggcag ggatgcgcgt aatcggacag gccattgatt accgggacgc cggacttttc
     1621 ggcaagcgtc acgacgtcac cgtgcttgaa aacgcgggcc atgatgatat cgaccatgcg
     1681 gcccaggacc tgggcggtgt cggaaacggt ctcgccacgg ccgagctggg tgtccttcgg
     1741 cgacaggaac agcgcgtgac cgcccatgcg ggccattgcg cattcaaacg aaacacgggt
     1801 gcgggttgac gacttttcga aaatcatcgc catcgtcttg ttgtgcaggg cgtcgccgta
     1861 acgctcggga tttgccttca tgtcggcggc aagggcgaaa atttcgtcca ggtcttccct
     1921 gtcaagatcc ttgatggcca aaagatggcg aaccttcatg acttcctcca gaaaccacag
     1981 tttgaaacaa gct
//
LOCUS       DAOPPA010000540         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_61437_length_1992_cov_4.218379, whole genome shotgun sequence.
ACCESSION   DAOPPA010000540 DAOPPA010000000
VERSION     DAOPPA010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61437_length_1992_cov_4.218379"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKH54_12935"
     CDS             complement(<1..991)
                     /locus_tag="PKH54_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00845.1"
                     /translation="MRLDVWKVFPIAAMLAVSLYACGGGPAATDVTDAVQDVPTDSLT
                     PDSGNDVIRPDANVDTGDTAGDEGRDTGDTGETGDDGNPDITDNGDDGNDPADTPDAP
                     DQVEPDAGDIHDDLPPDSTDVQTFQCISDDECIGKVVPGKCQVPWCNDDNECDLAMAE
                     DDTLCTDDSICTEADRCVDGECVGTPVDCADDDECTMDLCDQANFCFHRPYYGPCDDG
                     NKCTDNDSCGTGICVGTQITCNDDNTCTTDSCSPANGCVFTKISGTKCDDRDPCTASD
                     VCVAGECKGTFSCDDGNPCTFDGCNELGCTHEGGPLEGQICDDGLGCTEGDSCR"
     gene            1139..>1992
                     /locus_tag="PKH54_12940"
     CDS             1139..>1992
                     /locus_tag="PKH54_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00846.1"
                     /translation="MTDEELDTGIEEAVGPVEAAVDAIVDNVPDGRVGAVLAQSMRQR
                     LIVHVSGGLPEALAAVVADDPESGSLPSIPQSVTDDPDPARALVEWVQNEAGQLAGPL
                     GLAFSRLVTGFVVANRADIIQAMKDAGNGPAEDRQDGIVTASALINTATSMIEEIPGI
                     DSLDQATERMEAARDKLEEARVLLVGIQMDDAIMYGASAETETGLPAGVPELASGGRR
                     IDVANLLFDSWYREVDAHLMLAAKVGDPIDGTRVVDDGQRDYILDALVAVQEAFSVRP
                     DAPVLLRFG"
BASE COUNT          358 a          594 c          642 g          398 t
ORIGIN      
        1 cccggcagga atcgccctcg gtgcatccaa ggccgtcgtc gcagatctgg ccttccagcg
       61 gaccgccttc gtgcgtgcag ccaagttcgt tgcatccgtc gaacgtgcac ggattgccgt
      121 cgtcgcaact gaaggttccc ttgcactcgc ccgccacgca gacgtcggaa gccgtgcacg
      181 ggtcgcggtc gtcacacttg gtgcctgaaa tcttggtgaa cacacacccg ttggccgggc
      241 tgcacgaatc ggtcgtgcaa gtattgtcgt cattgcaggt tatctgggtg ccgacgcaga
      301 ttccggttcc gcatgaatcg ttatcggtgc atttgttgcc gtcgtcgcaa ggcccgtaat
      361 acggcctgtg gaaacagaag ttggcctggt cgcacagatc catggtgcat tcgtcatcgt
      421 ccgcgcagtc aaccggggtt ccgacgcact cgccgtcaac gcagcgatcc gcttcggtgc
      481 agatggaatc gtcggtgcag agcgtgtcgt cctcggccat cgcaaggtcg cattcgttgt
      541 cgtcgttgca ccacggaacc tggcattttc ccggaacgac ctttccgatg cattcgtcgt
      601 cggaaatgca ctggaaggtc tggacgtcag tcgagtccgg cggcaggtcg tcatggatat
      661 cgccagcgtc gggctccacc tgatccggag cgtccggcgt gtctgccgga tcgttcccgt
      721 cgtccccgtt gtccgtgatg tccggattgc cgtcgtcccc ggtttccccg gtgtcgccag
      781 tgtcccggcc ctcatccccg gcagtgtccc cggtgtccac gttcgcatcc ggccgaatga
      841 catcgttccc ggagtccggg gtcagggagt ccgtcgggac gtcctgcacg gcatcggtaa
      901 cgtcggtcgc cgccggtccg ccgccgcagg cgtaaaggga tacggcaagc atggccgcaa
      961 tggggaaaac cttccagaca tcgagtctca tcggtcacct ctgtgtcgtg ggcagggcca
     1021 cggtcaaaaa aagcattcaa gcttcgaatc tgtcgaacag taaacgaccg gcatataata
     1081 ctacatgtgc gcccgcgcgc gccacagtta gctggcatac atccggagaa acattcgaat
     1141 gacagatgaa gaactcgata ccggaattga agaggccgtc ggcccggttg aggccgccgt
     1201 cgatgcaatc gttgacaacg tgccggatgg cagggtcggg gccgttctgg cccagtcgat
     1261 gcggcagaga ctgatcgttc atgtgtccgg ggggctgccg gaggcactgg cggcggtcgt
     1321 tgccgacgat cccgagtcgg gatcactgcc ttcgattccg cagtcggtaa cggacgatcc
     1381 cgatccggcc agggcactgg tcgagtgggt gcagaatgag gccggccagc ttgccggccc
     1441 gctcggcctg gccttttcca ggctggtgac cggatttgtc gtggctaatc gcgcggatat
     1501 catccaggcc atgaaggatg ccggaaatgg ccccgccgaa gacaggcagg acggcattgt
     1561 cacggcctcg gcgttgataa acacggcaac gtcgatgatc gaggaaatac ctggcatcga
     1621 tagtctcgac caggccacgg aacggatgga agcggcccgg gacaagctgg aagaggctcg
     1681 tgtcctgctt gtcggaatcc agatggacga cgccatcatg tacggcgcaa gcgccgagac
     1741 cgagaccgga cttccggccg gggtgccgga actggcttcg ggcggtcgca ggattgacgt
     1801 tgccaacctg ttgttcgatt cgtggtatcg cgaggttgat gcgcacctta tgctggcggc
     1861 caaggtcggc gatccgatcg atggaaccag ggtcgtcgat gacggtcagc gcgactatat
     1921 actggatgcg ctggtggccg ttcaggaagc gttttccgtc aggccggacg cgccggtgct
     1981 gctgcgcttc gg
//
LOCUS       DAOPPA010000541         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_61784_length_1984_cov_3.400207, whole genome shotgun sequence.
ACCESSION   DAOPPA010000541 DAOPPA010000000
VERSION     DAOPPA010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61784_length_1984_cov_3.400207"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKH54_12945"
     CDS             complement(<1..753)
                     /locus_tag="PKH54_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00847.1"
                     /translation="MKVIVNAVVALAALVVMISGANAGEQMHQVTIDDVMKFRVINNV
                     SLLPSGDFILRVQDWGTDRSFAKDLYLLGADGRLRQLTFSGKLKGDFAVSPDGKSIVF
                     AGERDGKAGLYVLPLDGGESRLVLGMPIGVSSLRWAGNKVYFSAAVFPDCGIDFECTR
                     KRLEQKKDVTSAMVYDDLYVRPWSSWWDGTRNNLFAFDFATGVVDPVASGDFDVPTVP
                     WGGVEDYDISPDGKSVCYTAKKDPKKYLSTNDD"
     gene            complement(849..>1984)
                     /locus_tag="PKH54_12950"
     CDS             complement(849..>1984)
                     /locus_tag="PKH54_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00848.1"
                     /translation="AARPAPVPAPVAKPAPAPAAQPAPEQQDRAPRNVPAAFRADWKP
                     SAAPRPAPAATAKPSVQRPVAARPASDPGPSVASDGPRVDDELDSTLVQQPGQGGRPV
                     QPVTAVPPKPVSPGESSGRTVELEKALELERADRLQIAEALKVAQERYYDASERADIA
                     EAALIDVKSEMDLISQENREMADRADQLERENADLLQEIDDLRRERTENSSSASSVQQ
                     QISSLQAEVGRLKGELSRRELEVSSLKSELEQARTTTQPASSGDLDAALRQIEELKMS
                     NKGYLKRIGRLMEEQEKAVPCGVIVTPEVIGLADRLVDLAGESIDAASLARSLVSELK
                     AAGAADETVLEMRSAIASLADSVNDLRKVSADMQTMLKPPVQD"
BASE COUNT          390 a          624 c          566 g          404 t
ORIGIN      
        1 gtcgtcgttt gttgaaaggt acttcttggg atccttcttg gcggtatagc agaccgactt
       61 tccatcgggg gaaatgtcgt aatcctcgac cccgccccac ggaaccgtcg gaacgtcgaa
      121 atcaccgctt gccaccgggt caacaacgcc agttgcaaaa tcgaacgcaa acaggttgtt
      181 gcgcgtgccg tcccaccagg aactccatgg ccggacgtaa aggtcgtcgt aaaccatggc
      241 ggacgtcacg tccttcttct gttccaggcg ctttctggtg cattcgaagt cgattccgca
      301 atcagggaag accgccgccg aaaaatacac cttgttcccg gcccagcgca gcgaggaaac
      361 gccgatcggc attccaagga ccagccggct ttcgccgcca tcaagcggaa gaacgtacag
      421 accggccttg ccgtcgcgtt caccggcaaa gacaatcgac ttgccatccg gcgagaccgc
      481 gaagtcgccc ttcagctttc ccgagaaagt aagctggcgc agccttccgt cggcgccaag
      541 cagatacaga tcctttgcaa acgagcgatc ggttccccag tcctggaccc ggaggatgaa
      601 atcacccgac ggcagcaggc tgacattgtt gataacccgg aacttcatga cgtcgtcaat
      661 ggttacctga tgcatctgtt caccggcgtt tgcgcccgaa atcatgacaa ccagggctgc
      721 cagggccaca acagcgttaa cgattacctt cattcgaaac ctccagggga aatccgcagg
      781 gaacatggaa cgacacaata taacgcgatc catcccagtc gtgaatatca gtattgaatt
      841 acggtggatc agtcctggac gggcggcttg agcatcgtct gcatgtccgc cgaaaccttt
      901 cgaagatcgt tgacggaatc ggccaggctc gcgatggcgg atctcatttc aagaaccgtc
      961 tcgtccgcgg caccggcagc cttcagttcg gaaacaagac ttcgcgccag gctggctgcg
     1021 tcgatcgatt ccccggccag atcaaccaga cgatcggcca gtccgatgac ttccggagtc
     1081 acgatcacgc cgcatggaac cgccttctcc tgctcttcca tcaggcgccc gattcttttc
     1141 aggtagccct tgttggacat cttcagttct tctatctggc gcagggcggc atcaagatcg
     1201 cccgacgaag cgggttgcgt ggtcgtcctg gcctgctcca gttcactctt cagcgacgac
     1261 acctccagtt cccttctgga aagctcgccc ttcaacctgc cgacctctgc ctgaagcgac
     1321 gaaatctgct gctggactga gctcgcggac gaagagttct cggttctttc gcgacgcaaa
     1381 tcatcgattt cctgcaacag gtcagcattc tcacgttcaa gctggtcggc ccgatccgcc
     1441 atttcacggt tttcctggga aatcagatcc atttccgact tgacatcgat cagcgccgct
     1501 tcggcaatgt cggcacgctc ggatgcatcg tagtaacgct cttgggccac cttcagcgcc
     1561 tcggcaatct gaagacgatc tgcccgctcc agttcaagcg ccttttcaag ttcgacagtc
     1621 cggccggagg actcccctgg cgaaaccggc ttcgggggaa cggcagtcac tggctgaacc
     1681 gggcgaccgc cctgcccggg ctgctgaacc agggtgctgt cgagttcgtc gtcgacacgc
     1741 gggccatcgg atgcgacaga cgggccagga tcgctggcgg gcctggccgc taccggtctc
     1801 tggacggatg gcttcgcggt ggctgccggc gcgggccttg gtgccgctga aggcttccag
     1861 tcggccctga aggctgccgg aacattcctt ggagccctgt cctgctgttc gggggcgggt
     1921 tgcgccgccg gcgcgggcgc cggtttggcg acgggggccg gcacaggggc tggcctggcg
     1981 gctg
//
LOCUS       DAOPPA010000542         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_61930_length_1981_cov_2.922638, whole genome shotgun sequence.
ACCESSION   DAOPPA010000542 DAOPPA010000000
VERSION     DAOPPA010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61930_length_1981_cov_2.922638"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1981
                     /locus_tag="PKH54_12955"
     CDS             <1..>1981
                     /locus_tag="PKH54_12955"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOD00849.1"
                     /translation="GLWFDPGVSESADGRKPERLPADGAQAGDIAARVKDRPGRVESL
                     SGNDKAMPAPLLYDLTELQRHANRLYGLTAKDTLAVAQSLYEKHKLITYPRTDSRHLT
                     QDVAAGIGSVLAVVAPRYADMVAPDTGTRALGPRFVDDTKVSDHHAIIPTPTRPRLEA
                     LTKDEANVYDLVCRRLLMAWHDAYKTKVTSVVTVVESPGLDGSVIRDSFRSSGTVVIQ
                     WGWKILDIGTEKKVAKGKKARDAAKDAGGDEGGTGGDVSGGDDTDLPVGLSVGQERPV
                     TEVAVQTKETKPPKRFNDASLLTAMETAGKLLDDKDLENAMSERGLGTPATRAAILET
                     LINRKYVERMGKALKATDAGIQLISVVHESVRSPSMTGEWELRLKKMEKGQDSFPEFM
                     KGIEEYVIGVLGQVQRGPDPRLASRATAAGAAGGSAATPGGKAGMVTRTQNEAGPGSG
                     SMSVPVAAHGAGPGSVPAAGGPAGQASSEASATRPAPSGSPAWQPPDLLDILRNRFKF
                     QDFRPHQEAVCRSVALGHNSLLVMPTGSGKSLCYQLPGIARGGTTLVISPLIALMEDQ
                     VTHLSDMGFNARCLHSGLSREDSRNVCKEYLAGVLDFFMIAPERLSVTGFPEMLAKRP
                     PVLIAVDEAHCISSWGHDFRPDYRLLKDRIPMLMPA"
BASE COUNT          382 a          573 c          700 g          326 t
ORIGIN      
        1 cgggctatgg ttcgatcccg gcgtctcgga atcggccgac gggcgcaagc cggaaaggct
       61 gccggctgac ggcgcccagg ccggtgacat cgcggcgcgc gtcaaggacc ggccgggacg
      121 cgtcgaatcg ctgtcgggaa acgacaaggc gatgccggcg ccgcttcttt atgacctgac
      181 cgagcttcag cggcacgcaa acaggttgta tggactgacc gcgaaggaca cccttgcggt
      241 ggcccagtcc ctgtatgaaa agcacaagct gattacgtat ccgcgtaccg acagccgcca
      301 cctgacccag gacgtcgcgg ccggaattgg gtcggttctg gcggtggtgg cgccgcgtta
      361 cgccgacatg gtggcgccgg acacgggcac gcgtgcgctg gggccgcgct ttgtcgatga
      421 cacgaaggtt tccgatcacc acgccatcat cccgacaccg acccggccca ggctcgaagc
      481 gcttacaaag gatgaagcga acgtatatga ccttgtttgc cgacggctgc tgatggcctg
      541 gcacgacgcg tacaagacga aggtgacgtc ggtcgtgacg gttgtggaaa gcccggggct
      601 tgacgggtcg gtaattcgtg acagcttccg gtcgtccggg acggtcgtga tccagtgggg
      661 ctggaagatt ctggacatcg gcacggaaaa gaaggtcgcg aaggggaaga aggccaggga
      721 cgcggcgaag gacgccggcg gtgacgaggg cggaacgggt ggcgacgttt cgggcgggga
      781 cgacacggat ctgccggtcg ggctgtcggt cggccaggag cgaccggtta cggaagtcgc
      841 ggtacagaca aaggaaacca agccaccgaa gcgatttaac gacgccagcc tgctgaccgc
      901 gatggaaacc gctggaaagc tgctggatga caaggatctg gaaaacgcga tgagcgaacg
      961 cgggctgggg acgccggcga cgcgtgccgc catccttgaa acgctgataa acaggaagta
     1021 cgtcgaacga atgggcaagg ctctgaaggc caccgatgcc gggatccagt tgatttcggt
     1081 cgtccacgag tcggtgcgaa gtccttccat gacgggcgaa tgggaactgc gcctgaagaa
     1141 gatggaaaag ggtcaggaca gcttcccgga attcatgaag ggaatcgagg agtacgtcat
     1201 tggggtgctt ggccaggtcc aacgcgggcc tgaccccagg cttgcgtcca gggcgactgc
     1261 ggccggggct gcgggcggtt ccgcggcgac accgggcggc aaggcaggga tggtgacccg
     1321 gacgcaaaat gaagcggggc ctggttctgg gtcgatgtcg gtgcctgtgg cggcgcatgg
     1381 ggcggggccc ggttctgtgc cagcggccgg cgggcccgcg ggccaggcgt catcggaagc
     1441 gtccgcgacg cgccctgccc cgtcgggatc gccggcgtgg cagccccccg atttgctgga
     1501 tatcctgcga aacaggttca agttccagga cttcaggcca catcaggagg ccgtctgccg
     1561 gtcggtcgcc ctgggccaca actcacttct ggtcatgccg accggaagcg gcaagtcgct
     1621 ttgttaccag cttcccggaa tcgcccgcgg cggcacgacg cttgtcataa gcccgctgat
     1681 tgccctgatg gaagaccagg taacccatct gagcgacatg ggcttcaatg cgcggtgcct
     1741 gcattcggga ctttcgcgcg aggattcaag gaacgtctgc aaggagtacc tggccggggt
     1801 tctggacttc ttcatgattg cgcccgagcg cctgtccgtg acgggcttcc cggagatgct
     1861 ggcaaaacgg ccgccggtcc tgatcgccgt tgacgaggcc cactgcatca gttcatgggg
     1921 acatgacttc aggccggact accgcctgct gaaggaccgg attccgatgc tgatgcctgc
     1981 g
//
LOCUS       DAOPPA010000543         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_62103_length_1977_cov_2.822581, whole genome shotgun sequence.
ACCESSION   DAOPPA010000543 DAOPPA010000000
VERSION     DAOPPA010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62103_length_1977_cov_2.822581"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKH54_12960"
     CDS             complement(<1..357)
                     /locus_tag="PKH54_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOD00850.1"
                     /translation="MTERILIKLLEKTASVRLGVVILVLIIAASIAGTGTAYDVYHSW
                     WFAVLLGMFGANLVACTALGIIRGTTRVGSLITHIGILVVLAGVIAGSILGTRGILSL
                     EAGEVSDAFTTDDLTAR"
     gene            complement(350..1789)
                     /locus_tag="PKH54_12965"
     CDS             complement(350..1789)
                     /locus_tag="PKH54_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00851.1"
                     /translation="MNTKSFVVMAGTVFLCVLCSTGCGGSNQPAVDATTIDVHADSIG
                     SDLIDDSTPADTRQDTSGTDPGAADHGSEIAPDNGITDPGNADNGSDDIPLDGEFVIL
                     AWNNLGMHCYNPSFKRLAVLPPFNTIWAQVYRKAPSPELITSGITIEYSYPDNSYSAA
                     GTPAKSDFWEYAEQLFGSPLATDVGLTGKGLTGTFELKTDHWEAEGIPVTEFRDRDAT
                     VGGSPLTWNRYPYQLATVVVKRTSDGTELARSRVVTPVSSELNCQTCHSDSGAATVKY
                     PITPSGTDDTDLNILRLHDYLAKTDHESNQPVLCAKCHASNALGTLGNMGVPNLSKAI
                     HSRHRDIKEISEDTTGCYACHPGPETKCLRDVMTREGSVAGCVACHGNMAAVATNPTP
                     WINEPRCDSAQCHGGEVRQDKPLYNMSKGHHGVYCAACHDSPHAIAPSREANDGIKFM
                     ELQDGEGPVKDCTLCHGFKPSGEIHEEDD"
BASE COUNT          357 a          577 c          605 g          438 t
ORIGIN      
        1 ccttgccgtc aggtcgtcgg tcgtgaacgc gtcggatacc tcgcctgcct caagcgaaag
       61 aatgccgcgc gtccccagta ttgacccggc gataacgccg gcaagcacca ccaggatgcc
      121 gatatgcgta atcagcgaac caaccctggt agttccccgt atgattccga gggcggtgca
      181 ggccaccagg ttggccccga acatgccaag aagaaccgcg aaccaccacg agtgatagac
      241 gtcgtaggcg gtgccggtgc cggcaatcga cgcggcaatt atcagcacca ggattacaac
      301 acccagtctt accgaggcgg tcttctcgag cagctttata aggattcgtt cagtcatctt
      361 cttcgtgaat ttccccggaa ggcttgaatc cgtggcagag ggtgcagtcc ttgacggggc
      421 cttcgccgtc ctgaagttcc atgaacttga ttccgtcatt ggcctcgcgg cttggcgcaa
      481 ttgcgtgcgg gctgtcgtgg catgccgcgc aataaacgcc gtggtgtccc ttcgacatgt
      541 tgtacagggg tttgtcctgc ctgacttcgc caccgtggca ctgggccgag tcgcatcttg
      601 gctcgtttat ccacggcgtc gggttcgtgg ccaccgcggc catgtttcca tggcaggcaa
      661 cgcaaccggc gacggagcct tcgcgggtca tgacatcgcg aaggcacttc gtctccggcc
      721 ccgggtgaca ggcgtagcaa ccagttgtgt cctcggaaat ttccttgatg tcccggtgtc
      781 ttgaatgtat ggccttcgag aggttgggga cgcccatgtt gccaagggtt ccaagggcgt
      841 tcgatgcgtg gcatttcgcg caaagaaccg gctggttgct ttcgtggtct gtctttgcaa
      901 ggtaatcgtg caggcgcagg atgttcagat ccgtatcgtc cgtgccggaa ggcgtgatcg
      961 ggtatttcac cgttgccgcg ccggaatcgc tgtgacaggt ctggcagttg agctcgctgg
     1021 acactggcgt gaccaccctt gatcgtgcaa gttcggtgcc gtcggaagtt cgtttcacaa
     1081 cgacggttgc aagctggtac gggtaacggt tccacgtaag tgggctgccg ccgacggttg
     1141 catccctgtc cctgaattcc gtcacgggaa ttccctctgc ttcccagtgg tcggttttca
     1201 gttcgaaggt tccggtcaag ccttttcccg tcaggccgac atccgtggcc aggggcgagc
     1261 cgaagagttg ttcggcgtac tcccagaagt cggatttcgc gggggttccg gcagccgagt
     1321 aggaattgtc aggataggaa tactcgatcg tgattccgga tgtgatcagt tcgggactgg
     1381 gagccttcct gtaaacctgg gcccagatcg tgttgaaggg cggcagaacc gccaggcgct
     1441 tgaaggacgg gttatagcaa tgcatgccca ggttgttcca cgcaagaatg acgaattcgc
     1501 catccagggg aatgtcgtcc gaaccgttgt cggcgtttcc cggatccgtg atgccgttgt
     1561 ccggcgcaat ctcggatccg tgatccgccg cgcccgggtc ggtgccggag gtgtcctgcc
     1621 tggtgtcggc cggcgtggag tcgtcaatca agtccgatcc gattgagtcc gcgtggacat
     1681 caatcgtggt cgcgtcaacg gctggctggt tcgacccgcc gcagccggtc gagcaaagca
     1741 cgcaaagaaa aaccgttccc gccatcacca caaatgattt ggtattcatt cacctgtccc
     1801 cgaggatatt gaacaacatt gtacgccgac tggcgccccg ctcaagatcc cgttcgcccg
     1861 ggcggccagt catcagggat tcatcggcgg ctggcctgtc gcctgcatca cgttctgcca
     1921 cagcagatcg tccgggtcga tcttcttgcg tcgtccgacc gcggccctga tcgggac
//
LOCUS       DAOPPA010000544         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_62867_length_1959_cov_2.257353, whole genome shotgun sequence.
ACCESSION   DAOPPA010000544 DAOPPA010000000
VERSION     DAOPPA010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62867_length_1959_cov_2.257353"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKH54_12970"
     CDS             <1..474
                     /locus_tag="PKH54_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD00852.1"
                     /translation="ENHGFNMIMFAPESAFVIRAANSGSATLRGVGTSARVEWKWLEL
                     SANYTWLYSRLESTGARMPARPEHVFSGLAGFRKGPFRLFLAPSWQSGFFLDTYQSVR
                     EEDRFRLDLGLEIRPRPFLVMAVDFLNVTDKKDSIDFVQQPLPGFSAFASVRIEI"
     gene            471..1649
                     /locus_tag="PKH54_12975"
     CDS             471..1649
                     /locus_tag="PKH54_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00853.1"
                     /translation="MIFGCIRFFAAGLLVAVGLSGCIDGNVGSSDSSMLPDDSGLSLP
                     DTNMASPEGIIVWNDRVFVANANFAYQGASLVFKAGFITVIDSSTGSVVNRIPVPFPN
                     PQEMAIYGGRLWVLCSGSTTFDPVSSLVTISESGGLAGIELDGIDSASEPGVLIPIPS
                     SEGSIVGYPSGMLVDEGRAWLGSGTSASLFLADLGEGRLLRGAGDPIMISSDSGQNTV
                     SIGRGPAGTMVAALFNQDTVMLIDRIDGRVLDDPWAPASVGMEGQLDGILDVEIDDAG
                     GRIFLLLGMAGRIVSIDAATGPAGGVTEVAAGLVVPNRMKMIGGKLHVVCSGDNSLAV
                     IDPSGDEPVRRTGLPVNCNPWELDGGIVGDSIEIWLTCLKSSSVVRVRMDDPDILEIK
                     "
     gene            1646..>1959
                     /locus_tag="PKH54_12980"
     CDS             1646..>1959
                     /locus_tag="PKH54_12980"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOD00854.1"
                     /translation="MNRRQRPGPVVIACVAMAFVAGCGKDPASPPSIAPSNSTARVVS
                     LIPSATSILLDLGARSDLAGATRYCRLPAALADLPRVGGILDVSAEAVVALRPTLVIG
                     SPA"
BASE COUNT          342 a          533 c          622 g          462 t
ORIGIN      
        1 gaaaaccacg gattcaacat gatcatgttc gccccggaat cggccttcgt gataagggcg
       61 gcaaattccg gatccgccac gctccgcggc gtcgggacgt cggcccgggt cgaatggaag
      121 tggcttgaac tttcggcgaa ctacacctgg ctttattcca ggctcgaatc gaccggtgcc
      181 cggatgccgg cccgtccgga acacgtgttt tcgggacttg ccggattcag gaaggggccg
      241 ttcaggttgt tcctggcacc ttcgtggcag tccggcttct tcctggacac gtaccagtcg
      301 gttcgtgagg aggatcgctt caggctggat ctggggctgg agatacggcc acggccgttc
      361 ctggtaatgg ccgttgattt tctcaatgtc accgacaaga aggattcgat cgatttcgtg
      421 cagcagccgc tgccgggttt ttcggccttc gcgtccgtaa ggattgaaat atgattttcg
      481 gttgcatcag atttttcgcc gcagggcttc ttgttgccgt cggcctgtcg gggtgcatcg
      541 atggaaacgt cggttcgtcc gattcatcca tgttgccgga cgattcaggg ctgtcgctcc
      601 cggatactaa tatggcgtct ccggaaggta taattgtttg gaatgacagg gtttttgttg
      661 ccaatgcaaa ttttgcgtac cagggggcaa gcctggtctt caaggccgga tttataacgg
      721 ttatcgactc atcgaccggc agcgtcgtca accgtattcc ggttcctttt ccaaatcccc
      781 aggagatggc catatacggt ggccgcttgt gggttctatg ttccggatca accacgttcg
      841 atcctgtatc ctccctggtc acgatttccg aatcgggcgg tctcgccggg attgaactgg
      901 acggtattga ttccgcgtct gagccaggtg ttctgatacc gatcccatca tcagaaggtt
      961 cgatagtcgg gtatccatcc ggaatgctgg ttgatgaagg cagggcatgg cttggaagcg
     1021 gcacgtccgc ctcgttgttc cttgccgatc tcggggaagg ccggcttctt cgcggggctg
     1081 gggatccgat aatgatttct tcggattccg ggcagaatac ggtctccatc ggtcgcggcc
     1141 cggccggcac aatggtggca gccttgttca accaggacac ggtcatgctc atcgaccgga
     1201 tcgacggtcg cgtactggac gatccctggg ctccagccag tgtcggaatg gaaggccagc
     1261 tggatggaat cctggatgtc gagattgatg acgccggcgg caggatattc cttctgcttg
     1321 ggatggccgg ccggatcgtg tccatcgatg ccgcgacagg gccggcgggc ggggtgaccg
     1381 aagtggccgc cggactggtc gttcccaaca ggatgaagat gattggcggc aagcttcacg
     1441 tcgtgtgctc cggtgacaac agcctggcag tcatcgaccc ttccggggat gaacctgtgc
     1501 gaaggacggg cttgccggtc aactgcaatc cgtgggaact tgacggcggg attgtcgggg
     1561 actcaatcga aatctggctt acctgcctga agtcctcttc cgtggtcagg gttcgcatgg
     1621 atgatccgga catcctggag ataaaatgaa ccgccgacaa cgccccggtc cagtcgtgat
     1681 tgcgtgcgtg gcgatggcgt tcgtcgccgg ctgcgggaag gatccggcat caccgccttc
     1741 aatcgcgcct tccaactcga cggccagggt tgtatcgttg attcccagcg cgacgtccat
     1801 actgctggat cttggcgcac gttcggatct ggcgggtgcg acgcgatact gcaggctgcc
     1861 tgcggcgttg gcggatctgc ctcgggtggg tggcattctg gacgtgtcgg ccgaggcggt
     1921 ggttgccttg aggccaaccc tggtaatagg ttcccccgc
//
LOCUS       DAOPPA010000545         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63185_length_1951_cov_3.195148, whole genome shotgun sequence.
ACCESSION   DAOPPA010000545 DAOPPA010000000
VERSION     DAOPPA010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63185_length_1951_cov_3.195148"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1895
                     /locus_tag="PKH54_12985"
     CDS             198..1895
                     /locus_tag="PKH54_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOD00855.1"
                     /translation="MTSNQPRDNLGDRIPFVAMDALGILKPMIKKSDAHVLVGGNNLH
                     AETTIDAGLQFQADLAAFEAAMKLSMRQGWRGPVARLDPSNHEQFLKRNREWLEGHEA
                     YVRAPSANPMLALVTDVGRTSARVSLTVDMGGEITLDQMKWAIPYTEFDGEKLTDKKG
                     SLSLDGNLKDASEALHKGDVILVMPAIDAKAGPLPGVFRLVQFPAVQAAIVAMDQRTG
                     YIKAMTGGWDFDDSQYNRVYATRQTGSVVKPIYYSRAYDIGIPPSTVLSGAPFKDGNW
                     NPTGAKASEDMTLFTGLTRSENRISLRAYRLVLDSDSVDGINEWATRLGLVDAFKGYP
                     SEALGIEQKPANILKAYGVFASGGLDVEPTLVKTIGNDDGRLIKDNRSSRDPLTSIID
                     SIGIELEAASEARRRVITTEAAFITAANLRNVAREGTGRAARQLQRSVFGKTGTLSFD
                     VWFAGWTHELTTVAWMGQDNQNRFLGRNRFESGVVAARSALLLWLDFMEHAAGGRQPV
                     EDLDPPPPGIVFAEIDPQTGLLSRDEGIIMPHIAGTEPQELSPLPIETVPWVQAAYF"
BASE COUNT          435 a          564 c          590 g          362 t
ORIGIN      
        1 aggtgggagg cccagcgccc cagcgcccgt ccccggccca ggcggcgacc atcgcagcac
       61 ttccccagtc accatcttcg gttacgccat ggcggtcgcc cgacgagttg aaacaccgac
      121 gggaccgcgt catcaatcgc atgcagatgg ccggagcagt tacgtcagac atggcggccc
      181 gggccattga ggaaccggtg acatcaaacc agccaaggga caacctgggc gatcgcatcc
      241 cattcgtcgc gatggacgcc ctgggaatcc tgaagcccat gatcaagaaa tccgacgcac
      301 atgttcttgt tggtggaaac aatcttcacg ccgaaacgac gattgacgcc ggacttcagt
      361 tccaggcaga cctggcggcg ttcgaggccg cgatgaaact gtcaatgcgc cagggttggc
      421 gtgggcctgt tgccaggctt gacccatcga accacgaaca gttcctgaag cgcaacaggg
      481 aatggctgga gggacatgaa gcctacgtcc gggccccgag cgcgaacccc atgctggcac
      541 tggtaaccga cgtcggcagg acatcggccc gagtatccct gacagttgat atgggcgggg
      601 aaatcacgct cgatcagatg aaatgggcca ttccatacac ggaattcgac ggcgagaagt
      661 tgaccgacaa gaagggttcc ctgtcccttg acggcaacct taaggatgca tccgaagcct
      721 tgcacaaggg cgatgtaatc cttgtcatgc cggcaatcga cgcaaaggcc gggccgcttc
      781 ccggtgtctt ccggctggtg cagtttcctg cggtccaggc ggccattgtc gcgatggatc
      841 aaagaaccgg atacatcaag gcgatgaccg gcggatggga tttcgatgat tcacagtaca
      901 accgggtcta tgcgacaagg cagaccggca gtgttgtcaa gccgatttac tacagcaggg
      961 cctatgacat cgggattcct ccatcgacgg tactttccgg ggctccgttc aaggatggca
     1021 actggaatcc aaccggtgcg aaggcttccg aggacatgac gctttttacc gggttgaccc
     1081 gatcggaaaa caggatatcg cttcgagcct acagactggt cctggactcc gattcggtcg
     1141 atgggatcaa cgaatgggcg acccgacttg gactggttga cgctttcaag ggttacccgt
     1201 ccgaggctct cggaatcgaa cagaagccgg ccaacatcct gaaggcgtac ggcgttttcg
     1261 cgtcaggggg gctcgacgtc gagccgacac tggtaaagac aatcggcaac gacgatgggc
     1321 ggctgatcaa ggataacaga agctccaggg atccgcttac ttcgattatt gattcgatcg
     1381 gtattgaact ggaagctgct tcagaggcgc gcagacgggt gatcacgacc gaggcggcat
     1441 tcatcacggc ggctaacctg cgaaacgtgg cccgcgaggg aacgggacgg gcggcccgcc
     1501 aactgcagcg ctctgtgttc ggcaagacgg gaacgctgtc tttcgacgtc tggtttgccg
     1561 gatggacgca tgaactcacc accgtggcct ggatgggtca ggacaaccag aaccgctttc
     1621 ttggaagaaa ccggttcgaa agcggtgtcg ttgcagcacg ctcggccctt ctgctgtggc
     1681 tcgatttcat ggagcacgca gccggcggac gtcaaccggt agaagatctg gatccgccgc
     1741 cgcccggaat cgtgtttgcc gagattgacc cgcagaccgg attgttaagc agggatgaag
     1801 ggatcatcat gccgcacatc gcgggaacgg aaccacagga actatcccca cttccaatcg
     1861 aaacagtccc gtgggtccag gcggcttact tctgacatca acctgttcgg gtcaacgtca
     1921 accggccagt tcttcctcgg ccaccttgcg g
//
LOCUS       DAOPPA010000546         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63257_length_1949_cov_3.833157, whole genome shotgun sequence.
ACCESSION   DAOPPA010000546 DAOPPA010000000
VERSION     DAOPPA010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63257_length_1949_cov_3.833157"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKH54_12990"
     CDS             <1..519
                     /locus_tag="PKH54_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00856.1"
                     /translation="PCPMPAPRALMAADFRGKALEGSEAGLVTVSDIVLGSADDIVDC
                     DFNKDGTVDFRDYDTNECSEECSCREQCDKDLLCVEFNQYVEFGQWPARLGGESGVKV
                     WINSRESVPEFDPWAEDVPRQLVAITGTLRNLSFLDPYPWIIEPRCQDDIVLTGEPKP
                     ITQACVGSQDGE"
     gene            521..>1949
                     /locus_tag="PKH54_12995"
     CDS             521..>1949
                     /locus_tag="PKH54_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00857.1"
                     /translation="MRNLSIGRGWNRLAAGAVLALALVAGGRAAYAGDFMDTRLTWTF
                     GDDDLMANAGEVIPDSPIVGIGDRPGYEMFMDNLNSRTSGRENLTHLVLYKKMPGFLK
                     GLTTEAALVLRMDMGALTEGNNPGVTDVLQDDGSYLRAAYTWDRDNPDDKYFALTLFP
                     FSTERFRLGYLYDISWGGGNIFTNNRSGLAPGLKFDVHAKMKHVVADAFVGFKTARIK
                     QVVQEGSQEVEQISVKETNYGVLGGVGVDLFDWVRLDAGFGYFQQGKFERTALMIGQP
                     VFSFGLSSRLTVRQGLPIQTSIDYKLYRNHPDALDASSSRETYKKDKFSWSVSAEYNH
                     VWQQLESIEQYAKTKLQPAWAGAVQVQFKYDYMRIQLVGLIRNLEYILMNVPSLTPFV
                     AIPEAGTSTTPEYFAAATIDYRFDSAHVTPFITGGVQVPATFITENPRTVQVIRDQTR
                     RDRLPEGYGAKPIGQLRVGAEWELSK"
BASE COUNT          407 a          532 c          609 g          401 t
ORIGIN      
        1 ccttgtccga tgccggcgcc cagggccttg atggccgccg atttccgcgg gaaggcgctt
       61 gaaggaagcg aggccgggct tgtcactgtc agtgacatcg tcctggggtc cgcggacgac
      121 atcgtggatt gcgatttcaa caaggacggc accgttgatt tccgtgatta cgacacgaac
      181 gaatgcagcg aagaatgcag ttgcagggaa caatgcgaca aggatctgct ttgtgtcgag
      241 ttcaatcagt acgtcgaatt cggacagtgg ccggcaaggc ttggtggcga gtcgggcgtc
      301 aaggtctgga tcaacagccg tgaatccgtg ccggaattcg atccatgggc ggaggatgtt
      361 cccagacagc ttgtcgcgat cacgggaacc cttcgcaatc tgagctttct tgacccttat
      421 ccgtggatca ttgaaccccg gtgccaggac gacatcgtgc tgaccgggga accgaagccc
      481 ataacccagg cgtgtgttgg cagccaggat ggagaatagg atgcgaaatc tttcgatcgg
      541 gcgtggatgg aatcgacttg cggcgggcgc ggttcttgcc ctggctcttg tggcgggcgg
      601 ccgcgcggca tatgccggcg acttcatgga tacaaggctg acgtggacgt tcggtgatga
      661 cgatctgatg gccaatgcgg gcgaggtcat ccccgacagt cctatcgtcg gcatcggcga
      721 caggccgggt tacgagatgt tcatggataa cctgaacagc cgcacgtccg gtcgtgaaaa
      781 cctgacgcac ctggttcttt acaagaagat gcccggtttc ctgaaggggc tgacgaccga
      841 ggcggccctg gttcttcgga tggacatggg cgcgttgacc gagggcaata atcccggcgt
      901 gaccgatgtc ctgcaggatg acggttcgta cctgcgggcc gcatatacat gggatcgcga
      961 caatccggat gacaagtact ttgcgttgac gttgttcccg ttcagcaccg agcgtttcag
     1021 gctgggatac ctgtacgaca tatcgtgggg cggtggaaac atcttcacca acaacaggtc
     1081 gggcctggcc ccggggttga agttcgacgt gcacgcaaag atgaagcatg tcgttgccga
     1141 cgcgtttgtc ggcttcaaga ccgcccgaat caagcaggtt gtccaggaag gaagccagga
     1201 agtcgagcag atttccgtca aggaaaccaa ttacggcgtg cttggcggcg tcggggttga
     1261 tctgttcgac tgggtcaggc tggatgccgg tttcgggtat ttccagcagg gcaagttcga
     1321 aagaaccgcc ttgatgattg gccagcccgt cttttcgttc ggcctgtcgt cccgcctgac
     1381 cgttcgtcag ggcctgccga tccagacatc gatcgactac aagctctaca ggaaccatcc
     1441 cgatgccctg gacgccagtt ccagccgcga gacctacaag aaggacaagt tttcctggag
     1501 cgtgtcggca gaatacaacc atgtctggca gcagcttgaa agcatcgagc agtacgcgaa
     1561 gacgaagttg cagcccgcct gggccggggc ggtccaggtc cagttcaagt acgactacat
     1621 gagaatccag cttgtcgggt tgattcgcaa ccttgagtac atcctgatga acgttccaag
     1681 ccttacgccg tttgtcgcca ttcccgaggc cggaacatcg acgacgcccg aatacttcgc
     1741 cgccgcgacg atcgactacc gctttgattc ggcccatgtc acgccgttca tcaccggtgg
     1801 cgtgcaggtt ccggcgactt tcatcaccga aaatcccagg acggtccagg tcattcgcga
     1861 ccagacaagg cgtgatcgcc tgccggaagg ctatggggca aagccgattg ggcagcttcg
     1921 tgtgggcgcc gagtgggaac tgtcaaaga
//
LOCUS       DAOPPA010000547         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63261_length_1949_cov_3.512672, whole genome shotgun sequence.
ACCESSION   DAOPPA010000547 DAOPPA010000000
VERSION     DAOPPA010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63261_length_1949_cov_3.512672"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKH54_13000"
     CDS             <1..847
                     /locus_tag="PKH54_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD00858.1"
                     /translation="NVRTAAAQAIGDLAQVESIPALHKALNDREIAVKMAVIEAMGKI
                     RDREVIGRVSSLAFDPNQQVRLAALKVIADVNHKDAVAILRTAADDPNPAVRAMVLGA
                     MMKLDSNASLNRFERALVNLGERTFLEYAADMKDDFAPFAKAASTSQYQWARDAVLKA
                     MKYMPAVRINLLQEMALASNYEDTRIAALGVLARFDADIAFKIADAMMKDQSPRIRAA
                     AFRVIGQSAAPEVPQMLLAGIEDPDEYVKTVAAGAILERAAATKKGPRGSKAQPAPKK
                     AQGRK"
     gene            891..1523
                     /locus_tag="PKH54_13005"
     CDS             891..1523
                     /locus_tag="PKH54_13005"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD00859.1"
                     /translation="MKSSTYGAKTVLLSNGTATAEDLERVRRTVVDGGVIVYPAGTVY
                     GIGGNGFDPAVAQRIARIKGRDRTSFILLCDRLESALALGAIWPRAAMDLAARHWPGP
                     LTLVVPASDPVPPDLRATDGTVAIRVDAHPFSRALSAAAGCPLISTSANFSGRPPATS
                     WAEVEQTLLDACDLFVVDHGIIGGIPSTLVSFKDDRPVVLRHGAIPEDLL"
     gene            1520..>1949
                     /gene="truD"
                     /locus_tag="PKH54_13010"
     CDS             1520..>1949
                     /gene="truD"
                     /locus_tag="PKH54_13010"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOD00860.1"
                     /translation="MIDRNQLLDFLRGGADMAALPFASPDTPRVSGAIKTEPEDFMVD
                     EIPAYPFSGEGDHLMLNVRKRDIAGGDMIRIIARAAGIREEEIGTAGTKDRRAVTTQW
                     ISLPARAVEERGLFRPDDPDWPGVPGISVIQQTRHTNKLKP"
BASE COUNT          388 a          615 c          612 g          334 t
ORIGIN      
        1 gaatgtccgg actgccgcgg cgcaggctat cggcgatctg gcccaggtcg agtcgattcc
       61 ggccctgcac aaggcgctta acgatcgcga aattgccgtg aagatggccg ttattgaagc
      121 catgggcaaa atcagggatc gcgaggtcat cggtcgcgtc agttccctgg cattcgaccc
      181 gaaccagcag gttcggctgg ccgcgctgaa ggtgattgcg gacgtcaacc acaaggatgc
      241 cgtcgcaatc ctgcggactg ccgccgacga cccgaacccg gccgtcaggg cgatggtcct
      301 tggcgccatg atgaagctcg acagcaacgc ctccttgaac cgcttcgagc gggcccttgt
      361 gaatcttggc gagcgtacct tccttgaata cgccgccgac atgaaggacg atttcgcgcc
      421 gttcgcgaag gcggcctcga cctcccagta ccagtgggcc cgggacgccg tcctgaaggc
      481 catgaagtac atgccggcgg taaggatcaa ccttctgcag gaaatggcgc ttgcaagcaa
      541 ctatgaggac accaggatcg ccgcgcttgg cgtgcttgcc aggtttgacg ccgacatcgc
      601 cttcaagatc gccgacgcga tgatgaagga tcaaagcccc cggatccgtg ccgcggcgtt
      661 ccgcgttatc ggacagtcgg cggcacccga agttccccag atgctgcttg ccgggatcga
      721 ggatccggac gaatacgtca agaccgttgc ggccggtgca atcctggaac gcgccgccgc
      781 caccaagaag ggcccgcgtg gcagcaaggc ccagccggcg ccgaaaaagg cccagggccg
      841 caagtgatcc caccgggcgg actgaccgac cctttcccgg gcatcgaaca atgaaatcat
      901 caacctacgg cgcaaaaacc gtcctgctgt caaacggcac ggccacggcg gaagatcttg
      961 aacgggttcg caggaccgtc gttgatggcg gggtgatcgt ctatcccgcc ggcaccgttt
     1021 acggaatcgg cgggaacggc ttcgacccgg cggtggccca gcgaatcgcc cggattaagg
     1081 gtcgcgacag gacatcgttc atactgcttt gcgaccgact cgaatcggcc cttgcgcttg
     1141 gcgccatatg gccgcgggcg gcgatggatc ttgctgcaag gcactggccc ggtccgctga
     1201 cgctcgtcgt tccggcaagc gatccggtgc cgcccgatct gcgcgcaacc gacggaacgg
     1261 tggccatccg ggtcgatgca cacccgtttt cacgcgccct gtcggcggcg gccggatgtc
     1321 cgctgatttc aacaagcgcc aatttttcgg gaaggccgcc ggccacttcc tgggccgaag
     1381 tcgaacagac cctgcttgac gcatgcgatc tgttcgtcgt tgaccacggg atcatcggag
     1441 gcattccttc gacactggtt tcattcaagg atgaccgacc ggtcgtatta aggcacggcg
     1501 ccatcccgga ggatctgctg tgattgacag gaaccagctc ctggacttcc tcaggggggg
     1561 cgccgacatg gcggcccttc ccttcgcatc gccagacact ccacgggttt cgggggccat
     1621 caagacggaa ccggaagatt tcatggtcga tgagattccg gcttacccgt tttccggcga
     1681 aggcgaccac ctgatgctta acgtcaggaa gcgtgacatc gccggcggcg acatgattcg
     1741 catcatcgcc agggcggcgg gaatacgcga ggaagagatc ggaaccgccg gaaccaagga
     1801 caggcgggcc gtgacgaccc agtggatttc cctgccggca agggctgtcg aggaacgcgg
     1861 actcttcagg cccgacgatc cggactggcc gggggttccc ggcatatccg taatccagca
     1921 gacacggcac accaacaagc tgaagcccg
//
LOCUS       DAOPPA010000548         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63668_length_1940_cov_3.724138, whole genome shotgun sequence.
ACCESSION   DAOPPA010000548 DAOPPA010000000
VERSION     DAOPPA010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63668_length_1940_cov_3.724138"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="PKH54_13015"
     CDS             <1..1698
                     /locus_tag="PKH54_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00861.1"
                     /translation="DGHDGLLIGMDARQTVASLQDRSFSRNELMRIAQSSSAGQVRII
                     IDACFSGLGTSGKPIVQGLQPIVLAADSAVADPRFVVMTAAKEDQYAGPLPGATRPAF
                     SYLVLGALRGWADSNLDGMVTAAEAHGYVEQVFSSIIRDRKQQPTLVGEKDSMLSPAT
                     GEPAPDLGELILKLNKGFAVETQLPTVPKVSTVRALDADALKFDLNDVENLQEYDRVI
                     RADRSGLTPMEKARAWRDLAIKAPKYEKLAISRAQEWEKYQAEMDQAEQIRRQRAESR
                     DRDWEKLRKALPLEVVPEDDKRQWAKMFVDAYGTSLSDNPYASLLAPYLPRGTVPVLV
                     EFAMLGGGAFSMGSVSGMPNERPVRRMSVADFELSVTEVTVAQYRRCVEIGVCTEPGP
                     GRDCNWNREGVDDHPINCVDYEQATAFAKWAGGRLPTEVEWEYAAKSGGQDSAYPWGN
                     DSATCQRAIMDDPASGSGCGKGSTLPVCSKPAGNSVQGLCDMAGNVREWTSSEYVPPA
                     TESTPGFDSYGDATVKMVVRGGSWFNGPKFVRASSRGGVLPTERSVYTGFRVARDAP"
     gene            1728..>1940
                     /locus_tag="PKH54_13020"
     CDS             1728..>1940
                     /locus_tag="PKH54_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00862.1"
                     /translation="MNRNFTTVIAVVCSVATISAVVGCASGRPATTVTKPVSQAPELP
                     APVVTKLPDRGWVDVRVSVIGTDAESK"
BASE COUNT          368 a          585 c          617 g          370 t
ORIGIN      
        1 gacggacatg acggccttct tatcggcatg gacgcccgcc agacggtcgc aagccttcag
       61 gatcgatcgt tttcccgcaa tgaactgatg cggattgccc agtcatcgtc cgccggtcag
      121 gttcgcatta tcatcgacgc atgcttttcc ggcctgggca cgtccggaaa gccaatcgtc
      181 cagggcttgc agccaatcgt ccttgccgcc gattccgctg tggccgatcc ccggttcgtc
      241 gtaatgaccg ccgccaagga agatcagtac gccggcccgc ttcccggggc gacgcgtccg
      301 gccttcagtt acctggttct tggcgccttg cgcggctggg ccgattcgaa tcttgacggc
      361 atggtcacgg ccgcggaagc gcatggttat gtcgaacagg tcttttcgtc cataattcgt
      421 gaccgcaaac agcaaccgac acttgtgggc gaaaaggact cgatgctttc accggcaacc
      481 ggcgagccgg cgcccgatct tggcgaactg atcctgaagc tgaacaaggg ctttgccgtt
      541 gagacccagc ttccaacggt tcccaaggtc agtaccgtcc gcgcgctgga cgccgacgcc
      601 cttaagttcg acctgaacga cgttgagaat cttcaggaat acgaccgcgt cattcgcgcc
      661 gacagaagtg gcctgacccc gatggaaaag gcccgcgcgt ggcgcgatct ggcgatcaag
      721 gccccgaagt acgaaaagct tgcgatttcc cgcgcccagg aatgggaaaa gtaccaggcg
      781 gaaatggatc aggccgagca gattcgccgc cagcgcgcgg aaagccgtga tcgcgactgg
      841 gaaaagctgc gcaaggcgct tccgcttgaa gtcgtacctg aagatgacaa gcgtcagtgg
      901 gccaaaatgt tcgtggatgc ctatgggaca tcgctttccg acaacccgta cgcttcgctg
      961 cttgccccgt acctgccgcg gggaaccgtt cctgtcctgg tggaattcgc gatgctcggc
     1021 ggcggcgcct tctcgatggg ttccgtcagc gggatgccga acgaacgtcc ggtcaggcgg
     1081 atgagtgtcg cggattttga attgagcgtc acggaagtca ccgtcgccca gtacaggcga
     1141 tgcgtggaaa tcggcgtttg tactgaaccg gggcctggtc gcgactgcaa ctggaaccgt
     1201 gagggcgtcg atgaccatcc gattaactgc gtcgattacg aacaggccac cgctttcgcg
     1261 aagtgggcgg gtggccgcct tccgaccgaa gtcgagtggg aatacgcagc caagagcggc
     1321 ggccaggatt ccgcgtaccc gtggggcaac gattcggcga cctgtcagcg cgccatcatg
     1381 gacgatccgg caagcggttc cggctgcgga aaggggtcga cccttcccgt gtgttccaag
     1441 ccggccggta attccgtcca gggcctttgc gacatggccg gcaacgtccg cgagtggacc
     1501 agttccgaat acgtcccgcc ggcgaccgaa tccacccccg gtttcgacag ttatggcgat
     1561 gcgacggtga agatggtcgt acgcggcggg tcgtggttca acggaccgaa gttcgtgcgc
     1621 gcgtcaagtc gcggtggggt gcttcccacc gagcgatcgg tatataccgg cttcagggtt
     1681 gccagggacg cgccgtagtc gcgcgggttt tgtgaaaagg ggtatgaatg aaccgtaact
     1741 tcacgacagt tattgcggtc gtctgttccg tggcaacgat atccgcggtt gtcgggtgtg
     1801 cgtccggccg ccccgcgacg acggtgacga agccagtttc ccaggccccc gaactgcccg
     1861 ccccggtcgt caccaaactg ccggatcgcg gctgggtgga tgtgcgggtc agcgtcatcg
     1921 gaaccgacgc cgaatccaag
//
LOCUS       DAOPPA010000549         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63712_length_1939_cov_3.651274, whole genome shotgun sequence.
ACCESSION   DAOPPA010000549 DAOPPA010000000
VERSION     DAOPPA010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63712_length_1939_cov_3.651274"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKH54_13025"
     CDS             <1..270
                     /locus_tag="PKH54_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00863.1"
                     /translation="RISFFSDPDAGPPPEKGYVRMAMVRGGWEIRPAEGGGTSVRYEI
                     YTEPGGLVPALLVKEPLTEDALNYVRRCLKDAVPGKAVPEKAGSN"
     gene            complement(267..1604)
                     /gene="gdhA"
                     /locus_tag="PKH54_13030"
     CDS             complement(267..1604)
                     /gene="gdhA"
                     /locus_tag="PKH54_13030"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOD00864.1"
                     /translation="MTRQEFMEQVAARNPAQPEFLQAVAEVVDNIWDIVEANPKFKNG
                     NVLGRIVEPERVHTFRVAWVNDRGEVEVNRGYRIQFNSAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGGKGGADFNARGRSEGEIMRFCQAFMTELFKYIGPDTDV
                     PAGDIGVGGREIGFMFGQYRKLTHEFTGVLTGKGRTWGGSLIRPEATGFGVTYMADAM
                     LKRNKKSFEGMNVAVSGFGNVAWGACQKVTHLGGKVVTLSGPDGYVYDKDGVGTQEKW
                     DYLLELRASNNDRVEDYAKKFGCEFVAGRRPWERKVDVALPCATQNELGEEDARNLLK
                     NGCGCVVEGANMPCTPEAVHLFQQAKILFAPGKAANAGGVATSGLEMTQNSMRLSWNP
                     AEVDEKLHGIMNSIHEACVTYGKREDGTVDYVKGANVAGFMKVATAMVEQGVI"
BASE COUNT          358 a          570 c          596 g          415 t
ORIGIN      
        1 cggatttcct ttttttccga tccggacgcg gggccgccgc ccgaaaaggg atacgtcagg
       61 atggcgatgg ttcgtggcgg gtgggagatc cgtcccgccg aaggcggcgg cacctcggtt
      121 cggtatgaaa tctataccga gcctggtgga ctggttccag cgctgctggt gaaggaaccg
      181 ctgacggagg atgcgcttaa ctatgtaagg cgttgtctga aggatgcggt tccggggaag
      241 gcggttccgg aaaaagcagg ttcgaactag atgacgccct gttccaccat ggcggtggca
      301 accttcatga agccggcgac gttggcgccc ttgacgtagt caacggtgcc gtcttcgcgc
      361 ttgccgtaag tcacgcaggc ttcgtgaatc gaattcatga tgccgtgaag cttttcatct
      421 acttcggccg ggttccagct caggcgcatg ctgttctggg tcatttccag tccggacgtg
      481 gcgacgccgc cggcgttcgc ggcctttccg ggggcgaaca ggatcttcgc ctgctggaac
      541 aggtgcaccg cttccggcgt gcatggcatg ttggcgcctt cgaccacgca gccgcagccg
      601 tttttcagca ggttcctggc gtcttcctcg ccaagttcgt tctgggtcgc gcacggaagg
      661 gcgacatcga ccttcctttc ccacggacga cggccggcca cgaattcaca gccgaacttc
      721 ttcgcgtaat cttccacgcg gtcattgttg gaggcgcgca gttcaagcag gtagtcccac
      781 ttttcctggg ttccgacgcc gtccttgtca tagacgtatc cgtccgggcc ggacagggtc
      841 acgaccttgc cgcccagatg cgtgaccttc tggcaggcgc cccacgcgac gttgccgaat
      901 ccggaaacgg cgacgttcat gccttcgaac gacttcttgt ttctcttcag catcgcgtcg
      961 gccatgtagg tgacgccgaa tcccgtcgct tccggccgga tcaggcttcc gccccaggtg
     1021 cgtcccttgc cggtcagcac gccggtaaat tcgtgggtca gcttgcggta ctgtccgaac
     1081 atgaagccta tttcgcgtcc gccgacgccg atgtcgccag ccggaacgtc ggtgtccggg
     1141 ccgatgtact tgaacagttc ggtcatgaag gcctggcaga agcgcatgat ttcgccttcc
     1201 gaacggccac gggcgttgaa gtcggcaccg ccctttccgc cgcccatcgg cagcgtggtc
     1261 agtgcgttct tgaagatctg ttcgaatccc aggaacttaa gaatcgacag gttgacgctg
     1321 ggatggaagc gcagtccgcc cttgtacggg ccgatggcgc tgttgaactg gatgcggtaa
     1381 ccgcggttga cttcgacttc gccgcgatcg ttgacccagg ccacgcggaa agtgtggact
     1441 cgttcgggtt cgacgatcct tccaagcacg ttgccgttct tgaacttcgg gtttgcttcg
     1501 acgatgtccc agatgttatc gacaacttcc gcgaccgcct gaagaaactc gggctgagcc
     1561 gggttccttg ccgcaacctg ttccatgaat tcctgacgag tcatttaaga acctccagaa
     1621 acgagtacga gagaccccgg ggggcagggc gtggaacggg ggcaatagtg ccgcttaacc
     1681 gaccagaggc cgccggcccg gagggggcaa tctcacaccg cgtgaaacgc cttcaaccgc
     1741 ctgattccct agcccagaca gtaagataag tcaatacaaa tcgggcacgt ttcgtgcgac
     1801 actgtcggcg ttggggaggt cttcggaggt tacccgggaa catgctggtg acagaaatcg
     1861 gcccaagcct gaagaagttg aaggcgatcg aggatcgcgg atacgagctc accaggtggc
     1921 gggtcaatga aatcctttt
//
LOCUS       DAOPPA010000550         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63791_length_1937_cov_3.944740, whole genome shotgun sequence.
ACCESSION   DAOPPA010000550 DAOPPA010000000
VERSION     DAOPPA010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63791_length_1937_cov_3.944740"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1130)
                     /locus_tag="PKH54_13035"
     CDS             complement(84..1130)
                     /locus_tag="PKH54_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOD00865.1"
                     /translation="MKVALVGLPQSGKSTLFHALTGASGSSSKGGFTMGMVDVPDARV
                     DRLNEIYEPKKKTYARLEIVEAQAAAKSENRTGMDPAFLNAVKPMDALLLIVRAFDGP
                     DMCEPAADVKNVVSDMILTDLVAVEGRLERLESEKKKGRKVEPDEVAALERARDVLGA
                     DRLLITAPEVAAAPELRNFALLTAKPILVLVNVSDAEIGLPGDKALGMFGLPVMGLPT
                     FVCCAKVEEEIADLPPEERQEFQQALGIAEPALNMVIREVYGALGLISFLTGGKDEVR
                     AWTIQRGTLAPRAAGVIHSDIEKGFIRAEVIHYTDFDRLGSEAEARKAGKYRLEGKEY
                     PVGDGDIINFRFNV"
     gene            complement(1154..>1937)
                     /locus_tag="PKH54_13040"
     CDS             complement(1154..>1937)
                     /locus_tag="PKH54_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD00866.1"
                     /translation="PEAILAVLPSDHHVSDPETFREVLMAAVRHAERDKIVTLGITPT
                     HPETGYGYIRFGDFEPEPASVECRYRARTIQAFVEKPNLETAFSYLKAGRYLWNSGIF
                     IFKASVILNAFKEFLPDIFEQSEALLKLEAQATANSDAIYDSWERMNPISIDYGVMEK
                     VTHDVVVIPASFGWSDVGSWRALTAFPNDNQENFEKGRVVTVDCSDNVLYSTYGLLGT
                     IGLKGMVVVVTNGAVLVCPQDRTQDVKELVDEIERRDFTEYL"
BASE COUNT          350 a          576 c          586 g          425 t
ORIGIN      
        1 cgtggtgacc tcggatttat ttattatttt cgcgatgttt cgaagcgggc gaattttcat
       61 tcgcccttac ggatggcggg gggtcagaca ttaaaccgga agttaatgat gtcgccgtcg
      121 ccgacggggt attccttgcc ttccaggcgg tatttgccgg ccttgcgggc ttcggcttcg
      181 ctgccaaggc ggtcgaagtc cgtgtagtgg atgacttcgg cgcggatgaa gcccttttcg
      241 atgtcgctgt ggatgacgcc cgcggcacgt ggggcaagcg tgccgcgctg gattgtccag
      301 gcgcggactt cgtccttgcc gccggtcagg aaggaaatca ggcccagggc gccatagact
      361 tcgcggatga ccatgttcag cgccggctcg gcgatgccca gggcctgctg gaattcctgg
      421 cgctcctccg ggggcaggtc ggcgatttcc tcctcgacct tggcacagca cacgaacgtc
      481 ggcaggccca tgaccggcag cccgaacatg cccagcgcct tgtcgcccgg cagtccgatt
      541 tcggcgtccg acacgttgac cagaaccagg atcggcttgg ccgtcagcaa cgcgaaattg
      601 cgcagttccg gggcggccgc cacctcgggc gccgtaatca gcagccgatc ggcacccagg
      661 acgtcgcgcg cgcgttccag cgccgcgact tcgtccggtt cgaccttgcg tcccttcttc
      721 ttttcgcttt caagcctttc caggcggcct tccacggcca ccaggtcggt caggatcata
      781 tcggaaacca cgtttttcac gtccgccgcc ggttcgcaca tatccggccc gtcgaatgcc
      841 cgcacgatca gcagcagcgc gtccatcggc ttgaccgcgt tcaggaatgc cggatccatg
      901 ccggtacggt tttcggactt cgcggccgcc tgggcctcga cgatttccag acgggcataa
      961 gtctttttct tcggttcgta tatttcgttc aggcgatcga cccgggcgtc gggcacatcg
     1021 accatgccca tcgtgaagcc gcccttcgac gacgaaccgg atgcgcccgt aagcgcatgg
     1081 aaaagtgtgg atttaccgct ttgcggaaga ccgaccagcg caactttcat atggatgttc
     1141 ctcgtttgcg ggattacagg tattcggtga aatcacggcg ttcgatttcg tcaaccagtt
     1201 cctttacgtc ctgggtgcga tcctgggggc agacaagcac ggcgccattg gtgacaacga
     1261 caaccatgcc cttcagcccg attgtcccca gaaggccgta cgtggaataa aggacgttgt
     1321 cgctgcaatc gacggtcacg acgcggccct tttcgaagtt ttcctggttg tcgttcggga
     1381 atgccgtcag ggcgcgccac gagccgacgt ctgaccaacc gaaggatgcg ggaatcacca
     1441 caacgtcgtg cgtcaccttt tccatgacgc cgtagtcaat cgatatggga ttcatgcgtt
     1501 cccaggaatc atagatggca tccgagttgg ccgtggcctg ggcctcgagc ttcagaaggg
     1561 cttcgctttg ttcaaagatg tccggcagga actccttgaa ggcgtttagg ataaccgaag
     1621 ccttgaagat gaagatgccg ctgttccaaa ggtaacggcc ggccttcagg tatgagaagg
     1681 cggtttccag attcggcttt tccacgaaag cctggatcgt cctggcgcgg tatcggcact
     1741 cgacgctcgc cggttccggt tcgaagtcgc caaaacggat gtagccgtat ccggtctccg
     1801 gatgggtcgg cgtgatgcca agcgttacga tcttgtcgcg ttcggcgtga cgcacagcgg
     1861 ccatcaggac ttcgcggaaa gtttccgggt cgcttacatg gtgatccgac ggaagcacgg
     1921 caagtatggc ttccggg
//
LOCUS       DAOPPA010000551         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_63798_length_1937_cov_3.599894, whole genome shotgun sequence.
ACCESSION   DAOPPA010000551 DAOPPA010000000
VERSION     DAOPPA010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63798_length_1937_cov_3.599894"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..642)
                     /locus_tag="PKH54_13045"
     CDS             complement(37..642)
                     /locus_tag="PKH54_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00867.1"
                     /translation="MNPVTGRKVKVAGLVLAIAFVCVAMYLGIPHLTGGVHNLPANAA
                     SSPMALMEWTLRGVGNDEPQKLYNALGDDYRAVFGRFFDRVRAGHDQERWLAAAVKAG
                     VAEDMAGLKELPDNRMFMVWWNLPDRSVVDFELPLFFRQSAEAAESAIILKVFYETRD
                     VANAKTGFVLYQMDKGSDIVRTVRVGDGWRIDIPGDIPPGL"
     gene            complement(665..1258)
                     /locus_tag="PKH54_13050"
     CDS             complement(665..1258)
                     /locus_tag="PKH54_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00868.1"
                     /translation="MRPKIFRVAVLVAVLGGLGYLAWAGIPHLTGSNHDVPKEADGQA
                     LHMARWVIKAARDGEPQKLWNAMADSWRGTFIAAFDPIRKGSVSAEWLKAASDAGLAD
                     DAAEFAKLSDAKMFVAWWHLADRKIIGFRLPALFREPMDGKSEIELVKVFWETEPLKS
                     NECHLLYKIGDSTDLLRFVRQEGAWRLDDLKEVPAGM"
     gene            1417..>1937
                     /locus_tag="PKH54_13055"
     CDS             1417..>1937
                     /locus_tag="PKH54_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00869.1"
                     /translation="MNSRISGWLLPVILCGCGGSGGMPDSGLPFDLPAYFDVARSDLQ
                     EDRFPDDTTGDTDVMLPDDAFAIGQADILLEGHGLDEIDYDAGTDHGSNDAFIDVTGD
                     QGTHDSDEDAGAGDSWCTTATSCGPAATCNLSSGLCEDRSPGWTPFALLSAYPAAGAP
                     GDMIVIDGGGFNS"
BASE COUNT          387 a          626 c          541 g          383 t
ORIGIN      
        1 gaattttcac gcaggcgggc gaattttcat tcgcatctac aggccggggg ggatatcgcc
       61 gggaatgtcg atgcgccagc catcgccgac ccgtaccgtg cgcacgatgt cggaaccctt
      121 gtccatctgg taaagcacga aaccggtctt tgcgttcgcg acatccctgg tttcgtaaaa
      181 aaccttcagg atgatggccg actcggctgc ctcggccgac tgcctgaaga acaatggaag
      241 ttcaaaatcg accactgacc gatcgggcag gttccaccag accatgaaca tcctgttgtc
      301 cggaagctcc ttcagtcccg ccatatcctc ggccacgccg gccttcaccg cggccgcaag
      361 ccacctttcc tgatcgtgcc cggccctgac gcggtcgaaa aacctgccga agacggcccg
      421 gtagtcatcc ccgagtgcgt tatacagctt ctgcggttcg tcgttgccga ccccgcgcag
      481 ggtccattcc atcaaggcca tcggactgct ggcggcgttg gccggcagat tgtggacacc
      541 gccggtaaga tgcggaattc ccaggtacat cgcgacgcac acaaaggcga tggcaagtac
      601 caggcccgca accttgacct ttctgccggt aaccggattc aattgggcac ctcctccccg
      661 cgggtcacat gcccgccggg acttccttca ggtcgtcaag cctccatgcg ccttcctggc
      721 gcacgaaacg aagcagatcc gtcgaatcgc cgattttgta cagcaggtgg cattcgttcg
      781 acttcaacgg ttccgtttcc cagaacacct tgaccagttc gatttccgac ttgccgtcca
      841 tgggctcgcg gaaaagcgcc ggaagccgga aaccgatgat cttccggtcg gcaagatgcc
      901 accaggcaac gaacattttg gcgtcggaca gcttcgcgaa ttccgctgca tcgtcggcaa
      961 gaccggcatc cgaagcggcc ttcagccatt ccgccgacac gctgcccttg cgaatcgggt
     1021 caaaagccgc gatgaaggta ccgcgccacg aatcggccat cgcattccac aacttctgcg
     1081 gctcgccgtc acgggctgcc ttgatcaccc acctggccat gtgaagggcc tgtccgtcgg
     1141 cttccttcgg aacgtcatga ttcgacccgg taagatgcgg aatcccggcc catgcgagat
     1201 agccgaggcc gccaaggacc gcgaccagca ccgcaaccct gaaaatcttc ggtctcatcg
     1261 ggcacctccg tgcacaatga cgcacccccc tgcgccacgc agaaaccgat tctactggaa
     1321 ttggggatta tggaaatagg cggactggac acaatcgtca ccctcttatt gccaccgccg
     1381 gtttgtgcta gattggcgtt ccaaagaggt atcggcatga attcacgtat ttccgggtgg
     1441 ctgcttccag tcattttgtg cggctgcggc ggaagcggcg gtatgcccga cagcggcctg
     1501 cctttcgatc tgccggcata tttcgatgtc gcccgaagcg atctgcaaga agaccggttt
     1561 ccggacgaca ccaccggcga taccgacgtc atgcttcccg atgacgcctt cgctatcgga
     1621 caggccgaca tcctgttgga aggccatggc ctcgacgaga tcgattacga cgccggcacc
     1681 gaccacggtt cgaacgacgc gttcatcgat gtgacaggcg accagggaac gcacgactcg
     1741 gacgaggatg ccggtgctgg cgattcatgg tgcaccactg caacctcctg cggacctgcc
     1801 gcaacctgca acctttccag cgggttgtgc gaggatcgat ccccgggctg gactccgttc
     1861 gcgcttctgt cggcatatcc tgcagccgga gcgcccgggg acatgattgt gatcgacggc
     1921 gggggcttca attcttt
//
LOCUS       DAOPPA010000552         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_64416_length_1923_cov_3.845824, whole genome shotgun sequence.
ACCESSION   DAOPPA010000552 DAOPPA010000000
VERSION     DAOPPA010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64416_length_1923_cov_3.845824"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1162)
                     /locus_tag="PKH54_13060"
     CDS             complement(137..1162)
                     /locus_tag="PKH54_13060"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD00870.1"
                     /translation="MKKVLVFYGGMSREHDISMMTGRAVAASLADAGWTPVPVVISRD
                     GLFSFPGDDACQSAGPWYRFDQAVPMIAAIKPACVFIGLHGTFGEDGRIQSLCDLLDL
                     PYVGSDSIGSAIGIDKWATKAVFLAAGIPTSPARMLQGADLEDPAIVEAACLEIGFPM
                     VLKATREGSSFGVAIVRDSQGFQSALAAVRTCSDMILFEKFQKGREFSVPVIEDPLTG
                     LPRALPVIEIVVHKAEFFDYSTKYDPNAADEICPAPIEPDLAGRMSAAAIKAHRALML
                     SGYSRTDFIATADGCIWALETNTLPGMTAFSLLPKSAAVAGITYPELLDALIRRALAR
                     HAADSRS"
     gene            complement(1184..>1923)
                     /locus_tag="PKH54_13065"
     CDS             complement(1184..>1923)
                     /locus_tag="PKH54_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD00871.1"
                     /translation="LGAHSFPKGTHDRASHVDSIVSEWIPEVARGGLARFCDVFCENV
                     AFGVEDARRILRAAADHGLLLKVHAEQLSASGAARLAADMKAVSAEHLDFAGDRDIEA
                     LAAAGVVAVLLPGCPISLCNHKWVDARRFLRAGVRVALSTDFNPGSSVTMNLPLMGTF
                     AMSYMGMDAMQAWRSLTVDAAAALALPAGSGTLAVGAPADLVVFHCGDWRDPFYNYGM
                     NQVAAVIKAGVPVVRRTAAGEVQYLVP"
BASE COUNT          427 a          614 c          581 g          301 t
ORIGIN      
        1 gatatcggtg gactcggtgg gctcggtgga ctcgggctct gatcgcgaca aatccccgaa
       61 ccttcacgcc tgcgaccgac cttgaacgac gaatcccgac cacatcggaa cagacgccga
      121 tatcaattaa agactgttat gagcggctgt ccgccgcgtg gcgggcaaga gcccggcgga
      181 taagcgcgtc aagcagctcg gggtatgtga ttccggcgac cgcggccgac ttcgggagaa
      241 gggaaaaggc cgtcatcccg ggaagcgtgt tcgtttcaag tgcccagatg caaccgtcgg
      301 ccgtcgcgat gaaatccgtc cttgaatagc cggaaagcat cagtgcccgg tgcgccttga
      361 tggccgcggc cgacatccgt ccggccaggt cgggctcgat gggggccgga catatctcat
      421 cggccgcatt tgggtcatac ttggtgctgt agtcaaaaaa ttcggccttg tgcaccacaa
      481 tctcgattac aggaagcgca cgcggcaggc ccgttaacgg gtcttcaatc accggaaccg
      541 aaaactcacg acccttctgg aacttctcga aaaggatcat gtcggaacac gtgcgaacgg
      601 cggccagggc cgactggaaa ccctgggaat ccctgacaat ggcaacgccg aagcttgagc
      661 cttcacgggt ggccttcagc accatcggga agccaatctc gaggcaggcg gcttcgacaa
      721 tggcgggatc ttcaagatcc gcaccctgaa gcatgcgggc gggggatgtc ggtattcccg
      781 cggcaagaaa caccgccttg gtcgcccact tgtcaatccc gatggccgac ccgatcgagt
      841 cggagccgac gtacggcaga tcaagcagat cgcaaaggga ctggatgcgc ccgtcttccc
      901 cgaaggtacc gtgcagcccg atgaacacgc aggccggctt gatggcggca atcatcggaa
      961 ccgcctggtc gaacctgtac cagggaccgg ccgactggca tgcgtcgtcc cccggaaagg
     1021 aaaagagccc gtcgcgcgat atcaccacgg gcacgggagt ccacccggca tcggccaggc
     1081 tggccgcaac cgcccgtccg gtcatcatgg agatatcgtg ctcgcgcgac atcccgccat
     1141 agaacaccag aacctttttc attgtaaaaa caccttcaac cgattacgga accaggtact
     1201 gaacctcgcc cgcggcggtg cgccgcacga ccggaacacc ggccttgatg accgccgcaa
     1261 cctggttcat gccgtaatta tagaacgggt cccgccagtc gccgcagtgg aagacgacaa
     1321 gatcggccgg ggcgccgacg gcaagcgttc cagacccggc cggcagggcc agggcggctg
     1381 ccgcgtcaac ggtcagcgat cgccacgcct gcatcgcatc catccccatg tagctcatcg
     1441 cgaaggtacc catcagcgga aggttcatgg ttaccgacga cccgggatta aaatcggttg
     1501 aaagggcaac gcggaccccg gcgcgcagga acctgcgggc atcgacccat ttgtggttgc
     1561 acaggcttat cggacagccc ggaagaagca ccgcaacaac accggctgcg gccagcgcct
     1621 cgatgtcccg atcaccggca aaatcgagat gttcggcgga aaccgccttc atgtcggcgg
     1681 ccagcctcgc ggcgcccgag gcggaaagct gctcggcgtg aaccttgagc aacagcccgt
     1741 gatcggcagc ggcccgaagg atacgccggg cgtcctcgac accgaacgcg acgttctcgc
     1801 agaacacgtc gcagaacctg gcaagcccgc cgcgcgcgac ttccggaatc cactcggata
     1861 cgatcgaatc gacgtgcgaa gcccgatcat gagttccctt cggaaaggaa tgagccccaa
     1921 gaa
//
LOCUS       DAOPPA010000553         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_64898_length_1912_cov_3.865913, whole genome shotgun sequence.
ACCESSION   DAOPPA010000553 DAOPPA010000000
VERSION     DAOPPA010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64898_length_1912_cov_3.865913"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PKH54_13070"
     CDS             complement(<1..936)
                     /locus_tag="PKH54_13070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD00872.1"
                     /translation="MRPKMIGCLISAVILSQFAGGCGRSPNALDASTVSAVQILLDDY
                     VGFSHVPGVELTLIDPDGFEWQGSAGVADVRRGSPVDGDHLFRVGSHTKLFVGVVMMK
                     LVEQGLVDLDKPLTTYLPEYEKWSGVTVRMLLDMTSGIPDYLVIPGITMSMLTDPESS
                     MSPKQIVDAVHDGEMMFEPGTEGFYSDTNTFIAALIIEDVTGRPLARNIRRELTLPFG
                     LKDTFLDVNTFRKARLIHGYLDLTVAGNDLTIVDPGLLSLLPMAADLLPQDMLITRTL
                     VDSTYLAHPSYAWAAGALVSTPHDLASFVRSVFSGR"
     gene            1214..>1912
                     /locus_tag="PKH54_13075"
     CDS             1214..>1912
                     /locus_tag="PKH54_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00873.1"
                     /translation="MKRLTPVVLLCLAGLIPALVGCGGSNAEEDIVQGNDIQFGDYGS
                     EDDIATGDAVADIAPDTNGDADTAEVVPDTVLDAGSDAGQDLVVPDNTPVDNGTDNVE
                     PVDEGQDLPPVANFCNSLKDCESFEVCDLATGVCEERSSYNLIVGNEDLFQFKPAGGQ
                     YGDFVILDGSRFLTNPTTTAVRADIDKALIAGSSVAHSTISAHRILGVVPNKGGEVGV
                     VFQQATPVYQKYPDP"
BASE COUNT          438 a          561 c          538 g          375 t
ORIGIN      
        1 cctgccggaa aacacagacc tgacaaacga agccaggtcg tggggagtgc ttaccagggc
       61 gccggcagcc catgcatatg acgggtgggc cagatatgtc gaatcgacca gcgtccttgt
      121 tatcagcatg tcctggggaa gcaggtcggc ggccatagga agcagcgaga gaaggccggg
      181 atcgacgatt gtcaggtcgt tcccggcaac ggtcagatca aggtagccat ggatgagcct
      241 ggccttgcgg aaggtgttca cgtcaaggaa ggtgtccttc aggccaaacg gcagcgtcag
      301 ctcgcgtctg atgttgcggg ccaggggacg acctgtgaca tcctcgatta tcagggcggc
      361 gatgaacgtg ttggtatcgg aatagaagcc ttccgttcca ggctcgaaca tcatctcccc
      421 gtcatgcacc gcatcgacta tctgcttcgg cgacatgctg gattcgggat cggtcagcat
      481 cgacatcgtg atgccgggaa tgaccaggta atccggaatt ccggatgtca tgtccagaag
      541 catcctgacg gtcacgccgg accacttttc atactccgga agataggtgg tcaacggctt
      601 gtccaggtca acaagcccct gctcgaccag cttcatcatc acgacgccca cgaagagctt
      661 ggtgtggctg ccaaccctga agagatgatc gccatcgact ggactgcccc tgcggacatc
      721 ggccaccccg gcgctgccct gccattcgaa tccgtccgga tcgataagcg tcaattccac
      781 gcccggcaca tgcgaaaagc caacgtagtc gtcaagaaga atctgcacgg ccgacacggt
      841 acttgcatcg agggcattcg gcgatcgccc gcaacccccg gcaaactggg acaaaatgac
      901 cgccgaaatc aggcaaccaa tcattttagg acgcattttg aaagtcctcc cgcactcgta
      961 aaagcccaat gacacaccaa agatacaaca agacaattcc ctttgtaaac aagaggttgt
     1021 ctcgtgacac ttcgcgtcat aaccccttgc aaaccaagca gattcctgac ctttggggac
     1081 taaatccggt taacaatttc acaacatcac ataagaattt cggctccgcc gccgatacca
     1141 ccgggtgttg aaacaagacg tgactccctt taatctatac gcggctttga atggtcttct
     1201 cgtggagcat caaatgaaac ggctcacccc tgttgtcctg ctttgtctcg ccggccttat
     1261 cccggcgctt gtcggatgtg gcggctcgaa cgcggaggaa gatatcgttc agggcaacga
     1321 catccagttc ggggattatg gcagcgaaga cgacatcgcg accggtgacg ccgtcgctga
     1381 catcgcgccg gacacgaacg gcgatgccga cacggccgag gtggtgccgg ataccgtcct
     1441 tgacgccggt tccgacgctg gccaggatct ggtggttccg gacaacacgc cggtggataa
     1501 cggcaccgac aatgtcgaac cggttgacga gggccaggat cttccgcccg tggcaaactt
     1561 ctgcaattca ttgaaagact gtgaaagttt cgaagtctgc gacctggcca ccggcgtatg
     1621 cgaggaacga agctcgtata acctgatcgt cggcaacgag gatctgtttc agttcaagcc
     1681 ggcgggtggc cagtacgggg attttgtcat cctggacggc agccgattcc tgaccaaccc
     1741 gacgaccacc gcagtccgcg ccgatatcga caaggccctg attgccggtt cgtcggttgc
     1801 gcactcgacc atcagcgccc atcgcatact tggcgtcgtg ccgaacaagg gcggtgaggt
     1861 cggcgtcgtg ttccagcagg ccaccccggt ttatcagaag taccctgatc cg
//
LOCUS       DAOPPA010000554         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_65110_length_1907_cov_5.641469, whole genome shotgun sequence.
ACCESSION   DAOPPA010000554 DAOPPA010000000
VERSION     DAOPPA010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65110_length_1907_cov_5.641469"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKH54_13080"
     CDS             complement(<1..536)
                     /locus_tag="PKH54_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00874.1"
                     /translation="MEPQYATRTLFFRPEDNQRVRCDLCPHGCVLPEGQVGRCLVRRN
                     FGGQMVNLVWGRPGAMSVEPVGNLPLYHFLPRADAFCIGTAGCPLDCSFCRTHELART
                     MPLDMQFMLMPPEKVVFAARENKCRVIAFTFNDPTACAEYMMDVSEAAARADIRTVAV
                     SGSPLRLTLTPGCFYYHHG"
     gene            609..1022
                     /locus_tag="PKH54_13085"
     CDS             609..1022
                     /locus_tag="PKH54_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD00875.1"
                     /translation="MIKSEHEISLMRAAGRILADILQGLNERIVPGVTTADLDKWAEA
                     QISAAKCEPTFRGQPGMSRGARPFPAVACISLNEEIIHGIPSRQRVIQDGDVVSIDCG
                     VTYKGYVAGFQTLTPPAPGHSRLIKVSCGWNFLHA"
     gene            997..1548
                     /locus_tag="PKH54_13090"
     CDS             997..1548
                     /locus_tag="PKH54_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00876.1"
                     /translation="MDGISCTHEKDLRNLMANVVMKYDCCAIYASWLLKAIWMKAQED
                     TWRGQVLHTGVSDEVVMSDFKSFFDSVTASRVESSPLEIAEYWAKRRGKGAPTADGVV
                     HEQDWERIVLAADRGDAVTGEVLGRFRNGWSVIIDSVAGWLPDDWVSAAGTTVAVGRI
                     ELVVLRYDRVTEELVLRPRLPGE"
     gene            1705..1872
                     /locus_tag="PKH54_13095"
     CDS             1705..1872
                     /locus_tag="PKH54_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOD00877.1"
                     /translation="MRMDRFTTKAREAIEAAVQDATARGNPEIDVEHLLAAILTEKEG
                     VVASVPAAMHP"
BASE COUNT          394 a          522 c          603 g          388 t
ORIGIN      
        1 ccatggtggt agtaaaagca acctggggtc agggtcaggc gtaggggcga accgcttacg
       61 gcgacagtcc tgatgtctgc cctggccgcg gcctcgctga cgtccatcat gtattcggcg
      121 cacgccgtcg ggtcattgaa cgtgaacgct atgacccggc acttgttttc gcgtgccgcg
      181 aacacgacct tttcgggcgg catcagcatg aactgcatgt ccaggggcat ggtccgggcc
      241 agttcgtgcg tccggcagaa cgagcagtcc agcgggcagc cggccgtccc gatgcagaat
      301 gcgtcggcgc gcggaagaaa gtggtacaac ggcaggtttc cgactggttc aactgacatc
      361 gcgccgggcc ggccccacac aagattgacc atctggccgc cgaaattccg gcggaccagg
      421 catcttccga cctgtccctc gggaagtacg catccatgcg gacacaggtc gcatcgcacc
      481 ctctggttgt cctcgggcct gaaaaataat gtccgagttg cgtactgtgg ttccatccgg
      541 ctccttgcga agtcccaagg tcggcgctag tgtagcaccg gacttcgcgg aggcagaacg
      601 cggtgataat gatcaagtcc gaacacgaga tttcgctgat gcgcgcggcc gggcggatac
      661 tggccgatat actgcaggga ttgaacgaaa ggatagtccc gggcgtcacg acggcggacc
      721 tcgacaaatg ggccgaagcg cagatcagcg ctgcgaaatg tgaacctact ttccggggac
      781 agcccggcat gtcgcggggg gccagacctt ttccggccgt cgcctgcatt tccctgaacg
      841 aggaaatcat tcacggcata ccgtccaggc agcgagtgat tcaggacggc gacgtcgtca
      901 gcatcgactg cggcgtcacc tacaagggtt acgtggccgg attccagact ttgacacccc
      961 cggctcccgg ccatagtcgt cttattaagg tctcttgtgg atggaatttc ctgcacgcat
     1021 gaaaaagatt tacgtaactt gatggccaat gttgtcatga aatatgattg ttgcgcgatt
     1081 tacgcaagtt ggttgctgaa agcaatttgg atgaaggcgc aagaggatac atggcgggga
     1141 caagttcttc atactggggt gtctgacgag gtcgtcatga gcgatttcaa atcattcttt
     1201 gactcggtca cagcatcacg cgtggaatct tcgccgctcg agatcgctga gtactgggcc
     1261 aagcgcaggg gcaagggcgc gccaacagcc gatggcgtcg tgcacgaaca ggactgggaa
     1321 cggattgtcc tcgctgccga tcggggcgat gctgtaacgg gtgaagttct cgggcgtttc
     1381 cgcaacgggt ggtctgttat catcgactcc gtcgcaggtt ggttgcctga tgattgggtt
     1441 tctgccgcag ggacgacggt tgccgtgggt cggatcgaat tagtggtctt gcgatatgac
     1501 cgcgtgaccg aggagcttgt gttgcgaccg cggcttcccg gtgagtgatt aactgaattc
     1561 ctttagcggc cgttcccggc ttcgtgcggg cgaatcacat tttcctgcgt ccgccacatc
     1621 ttgcgccaat accctgccaa ccaaatagtc ttagcggtcg cggcgcacgg gatacgtgac
     1681 ggaggccgcg ccggaggtac ggatatgaga atggacagat tcacgacgaa ggcccgggaa
     1741 gcgatcgaag cggcggtgca ggacgcgacg gcgcgcggca acccggagat agacgttgaa
     1801 cacctgctgg cagcgatact tacggaaaag gaaggcgtgg tcgccagcgt gcctgctgca
     1861 atgcatccat aagcaccgaa cgaacggttc aagtttgtca gcgctcg
//
LOCUS       DAOPPA010000555         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_65122_length_1907_cov_3.615551, whole genome shotgun sequence.
ACCESSION   DAOPPA010000555 DAOPPA010000000
VERSION     DAOPPA010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65122_length_1907_cov_3.615551"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1289
                     /locus_tag="PKH54_13100"
     CDS             162..1289
                     /locus_tag="PKH54_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD00878.1"
                     /translation="MAKIDPTLAGKLKKYGAKDFPACYNCGNCTAVCSLTAKDANYPR
                     IFLRYGSLGMKDEILSAREPWLCYACGECSAACPRDAGPGDFMAAARKFAIAHHEPTG
                     LTRLLFTNSPFAVVFTVLLAVVMGTFMMMVSDRVDPDNVLKRWIFDHLSFSVIHDMGM
                     VIFTFMGLSALLGMILMGRRLFAGMPKLENPRKAIVSAIHNVRAELLTMRRYRKCQDD
                     EEPYFKDRKWPFQPWFVHWSIMWGFIGLLFATILDFLLKDPATTTWWPSRILGTVAGI
                     AMMWGATLAIIYRIKGVARQYRNSTLADWALLGALFAAGLTGFWMEVAVMAHLENDLN
                     AAIFIIHTIVSMELVLLFAFSKFAHAVYRPLALFNYFLRQK"
     gene            1336..>1907
                     /locus_tag="PKH54_13105"
     CDS             1336..>1907
                     /locus_tag="PKH54_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOD00879.1"
                     /translation="MKILVVYATHSGCTEDVARRIGTALRQGGAEVKVIPVESAPAPA
                     GFDAVIVGSGVRAGKWHGPAIRWIEKHAAAVRAIPHAMFTVCLMMRDGQKKAPEVLAF
                     TQPVFDRLDLKPVGVGLFPGWFVPEKFPLFERIILKMMKTPRGDFRDFDAVDSWVSVV
                     APRLGLAISIDDVPENQAEPVADGDSSLQV"
BASE COUNT          342 a          526 c          606 g          433 t
ORIGIN      
        1 cgatatcgat tcccgacatc gcgaaggcgt ccggcctgga tgcttcactg gtcaccaggc
       61 acgtcatgac gatgaacagg taccgcgtcg ccgagccggc cggccttgac gacagcgagc
      121 agtactacct ttaccagcag aagagctgac gggggcccgg gatggcaaag attgatccga
      181 cactggccgg gaaactgaag aagtacggcg cgaaagactt tccggcctgc tataactgcg
      241 gaaactgcac ggcggtttgc agcctgacgg cgaaggatgc caattatcct cgaatcttcc
      301 tgcgttacgg ctcgcttggc atgaaggacg agattctttc ggcccgggaa ccatggctct
      361 gttatgcctg cggcgagtgc agtgccgcgt gtccgcgcga tgccggaccc ggggacttca
      421 tggccgccgc gcgcaagttc gccatcgccc atcacgagcc gaccggcctg acgcgcctgc
      481 tttttacgaa cagcccgttt gcagtcgtgt tcacggtgct gctggccgtc gtcatgggca
      541 ctttcatgat gatggtttcg gatcgcgtcg atccggataa cgtgctgaaa cgctggattt
      601 ttgaccacct ttcattttca gtcatccacg acatggggat ggtcatcttc acgttcatgg
      661 gcctgtcggc cctgctggga atgattctga tggggcgtcg cctgtttgcc ggaatgccga
      721 agctggagaa tccgcgaaag gcaatcgtca gcgcaattca caacgtcagg gccgaactgc
      781 tgacaatgag gcgttaccgc aagtgccagg acgacgagga gccgtatttc aaggacagga
      841 agtggccctt ccagccgtgg ttcgtccact ggagcatcat gtggggcttc atcggcctgc
      901 ttttcgcgac catcctggac ttcctgctga aggatcccgc gacgacgacc tggtggccaa
      961 gcaggatact gggcacggtt gccggcatcg ccatgatgtg gggcgcgacg ctggcgatca
     1021 tttaccggat aaagggtgtc gcccggcagt accgcaattc gaccctggcc gactgggccc
     1081 tgctcggggc gctttttgcg gccggcctga caggtttctg gatggaagtc gcggtcatgg
     1141 cgcaccttga aaacgacctg aacgcggcga tcttcattat tcacaccatc gtctcaatgg
     1201 aactggtgct gctgttcgcc ttctcgaagt ttgcccatgc cgtttatcgt ccgcttgcct
     1261 tgttcaatta tttccttcgt cagaaatgat tcttccggtg gtatccttca tgcggcttgt
     1321 tttgcggggg cggcgatgaa gattctggtc gtttatgcaa cgcattccgg gtgtaccgag
     1381 gatgtggctc gtcgtatcgg cacggcgctt cgacagggcg gggcggaggt caaggtgatt
     1441 ccggtcgagt cggctccggc ccctgccggg ttcgatgcgg tgattgtcgg ttccggtgtg
     1501 cgggccggca agtggcacgg ccccgcgatc aggtggattg agaagcatgc ggccgccgtc
     1561 agggcaattc cgcacgcgat gtttacggtc tgcctcatga tgagggatgg ccagaagaag
     1621 gcgccggaag tccttgcctt cacacagcct gtcttcgatc gtcttgacct gaagccggtc
     1681 ggggtcgggc tgtttccagg ttggtttgtg ccggaaaaat tcccgttgtt cgagcggatc
     1741 atcttgaaga tgatgaagac gccaaggggt gatttccggg atttcgacgc tgtcgattcg
     1801 tgggtgtccg tggtcgcgcc gcggcttggc ctggcgattt ccattgatga tgtacccgaa
     1861 aatcaggcgg aacccgtcgc ggacggggat tcgtcgctgc aggtgga
//
LOCUS       DAOPPA010000556         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_65205_length_1905_cov_3.644865, whole genome shotgun sequence.
ACCESSION   DAOPPA010000556 DAOPPA010000000
VERSION     DAOPPA010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65205_length_1905_cov_3.644865"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKH54_13110"
     CDS             complement(<1..438)
                     /locus_tag="PKH54_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00880.1"
                     /translation="MHNSVLAALAVFALVIAGGCREKQPYPGAEQIRQNLVNEMKTAR
                     LPDVRQDEPSDPETVKPMLALALIHTAKGTTIVLGREDGAGLSWSQLPGHAWLTADGS
                     LRVARPEIRPARQVTRKKRSMMAGLVMEEYRQDSPPSRPIDLVP"
     gene            complement(492..1424)
                     /locus_tag="PKH54_13115"
     CDS             complement(492..1424)
                     /locus_tag="PKH54_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOD00881.1"
                     /translation="MKGKDYYAILGVGRDAGADELKKAYRKLAMQYHPDRNKGDKAAE
                     ERFKQINEAYAVLSDEKKRKEYDTFGAEGFGQRFSQEDIFRGFDFSSIFGGGEGFRGG
                     GFRSQGFGGPGAGFEDLLSQMFAGSGMGGRRHARPSRGQDMEQAVSLGFLEAMKGAAL
                     DVQIPDGAGSWEKVTVRIPPGVSSGTRLRVRGKGGHSPSGAERGDLFLVIQVEEHPVF
                     SRSGSDLSCDVLVPVSVMVLGGSADVPILEGVRKVKIAPGTQPGTRIRLKGHGVPSKS
                     GSAGDLYARIQPLFPDKVTDRARQLFSELADQGW"
     gene            1590..>1905
                     /locus_tag="PKH54_13120"
     CDS             1590..>1905
                     /locus_tag="PKH54_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00882.1"
                     /translation="MNSTSTVPTVVRATLMAACLLCACASDVNDPGQDVAAAPDSVTD
                     QDSMYIEFDAIVLSDGWTEIGGQSDQGTAYDTGVPVDTNEFADSTSLDIPVLRDSWAD
                     AST"
BASE COUNT          394 a          622 c          508 g          381 t
ORIGIN      
        1 gggaaccagg tcaatcggcc gggaaggggg ggagtcttgt cgatactcct ccatcaccag
       61 accggccatc attgaacgct tcttccttgt gacctgcctg gccggccgaa tctccggtct
      121 tgcaacgcga aggctcccat cggccgtcag ccaggcgtgc cccggcaact gggaccacga
      181 aagacctgca ccgtcttcgc ggcccaaaac gatggttgtg cccttcgccg tgtgaatcaa
      241 agcgagtgcc agcatcggtt tgacggtctc gggatcggaa ggctcgtcct ggcgaacgtc
      301 cggcaaacgg gcggtcttca tttcattcac aagattctgc ctgatctgct cggcccccgg
      361 gtatggctgc ttttccctgc aacctccggc aataaccagt gcaaaaaccg caagtgccgc
      421 caggactgag ttgtgcatct gaatcaccag ggcataagga cagcaaaacc ttacaacaga
      481 atcaggaaaa gctaccaacc ctggtcggca agttccgaaa aaagctgccg cgccctgtcc
      541 gtgaccttgt cggggaacaa cggctggatc cgggcataca aatcccccgc cgaacctgat
      601 ttcgagggca cgccatggcc cttgagcctg attctggtgc caggttgtgt gccgggagca
      661 atcttgacct tcctgacacc ctccaggatc ggcacgtctg cggatccccc aagcaccatg
      721 accgacaccg ggacgaggac gtcacacgac agatcgcttc cggatcttga aaagaccgga
      781 tgctcctcta cctgaatgac caggaacagg tcaccacgtt ccgcgccgga cggtgaatgt
      841 ccgcccttgc cgcgcacccg cagcctcgtc ccggaactca cgcccggcgg tatcctgacc
      901 gtaaccttct cccagcttcc ggcaccgtcc ggtatctgta catcaagcgc ggcacctttc
      961 attgcctcca ggaatccaag actgaccgcc tgctccatgt cctgtccgcg ggaaggcctt
     1021 gcgtgtcgcc tgccgcccat gcccgaaccg gcgaacatct gcgacaggag atcctcgaat
     1081 ccagcgcccg gaccgccgaa gccctgcgac ctgaaaccac cgcccctgaa cccctcgccg
     1141 cccccgaaga tggacgaaaa atcaaacccc ctgaagatgt cttcctggct gaaacgctga
     1201 ccgaaaccct cggcgccaaa cgtgtcgtat tccttgcgtt ttttctcgtc ggacaaaacc
     1261 gcgtacgcct cgttgatctg cttgaatctc tcctcggcag ccttgtctcc cttgttgcgg
     1321 tcgggatggt actgcatcgc aagtttgcga taagccttct tcaactcgtc ggcgccagcg
     1381 tcccttccga cccccagaat cgcgtaatag tccttgcctt tcataagttg caaacctccg
     1441 cggctggaat tatgtgttcg atccgtccgg aacggaagat gtcatcagtg gatcatggcg
     1501 gcgaaatgat gacattatct gtcaccaact gtgcgttcga ctcccggaat caatataata
     1561 cggccggaat gtgcctttga gcagatgaag tgaacagtac atcgacagtt ccgacagtcg
     1621 tgcgcgcaac gttgatggcg gcgtgtctcc tgtgcgcgtg cgcttccgac gtgaacgacc
     1681 ccggacagga cgttgcggcc gcgccggact cggtcaccga ccaggattcc atgtacatcg
     1741 aattcgatgc aatcgtactt tccgacggat ggacggaaat cggcggccag tccgaccagg
     1801 ggacggctta cgataccggt gttcccgtcg atacgaacga attcgcggac tccacgtccc
     1861 tggacattcc cgtgctccgc gactcctggg cggacgcttc gacgg
//
LOCUS       DAOPPA010000557         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_65944_length_1890_cov_3.747684, whole genome shotgun sequence.
ACCESSION   DAOPPA010000557 DAOPPA010000000
VERSION     DAOPPA010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65944_length_1890_cov_3.747684"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..>1890)
                     /locus_tag="PKH54_13125"
     CDS             complement(20..>1890)
                     /locus_tag="PKH54_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00883.1"
                     /translation="GTGKLDYPKVANNGMIMTFVFEYGIPLFFLAAGVGIENTSWYKD
                     LMADDDLVFAIIAVAGFLLAGENAAEIGLGGSPKAVLSRSATLVGNLILSKACSKLAT
                     YVTKKLTEAELEGAIPFVGWGLRMLSVSATLSAVAQTTAEVTSSPWVIKNGVTPTMDM
                     QVTIFHDPDDYQFPATATKYQVIATYADKKHRILPMRALPGTTVSDPIVVEFEDVPAG
                     GTVEVTVGFFAQNNWLAGRGSTGRVANLVPSGQDKLKLDITLEEIKVPLDQTTNYHHK
                     QKLNYASGKYQWVAGDAPTETLSSLSCANVGSHLCSLDNITFSQRAGMFGYSWQAAGP
                     NMAQCDTGTTDMQLHFVQNMSAKADPNIGYKTLDCGMSEKTTIVYELMGPENGTGMNF
                     FLDTRNGNYHLRRVVADESTPMSSSGLSWGRFTQPLDDIAINDAGYVVGANWSNSKLE
                     ILTIPAQGSSDDAAPWARLLGGEGTREGLMYGPKAIAFTAEGVLLVLETLNKRIQALD
                     IDGNPVKYFNDKQDYFADLFADTELVTYLDMGVEFMGYIYVLSYVNDGSNVNDYRLDI
                     YDPQGEFLSRTIGVAAHKMTVDFWRNVYSLNYQSIAGPGGRTEPSVSEWIPSTPNP"
BASE COUNT          352 a          563 c          556 g          419 t
ORIGIN      
        1 ggtcgaacca ggcacgtcgt tacggattgg gggtggaagg aatccattcg ctgaccgaag
       61 gttcggtcct gccacctggg cccgcaatgc tctggtaatt caggctgtac acgtttctcc
      121 agaaatcgac cgtcatcttg tgggccgcca cgccgattgt gcggctcaag aattcgccct
      181 gcgggtcgta gatgtccagc cggtagtcgt taacgttcga gccgtcgttg acgtacgaaa
      241 gaacatagat gtaacccatg aattcgacgc ccatgtccag gtacgttaca agttccgtat
      301 ccgcgaacag atccgcgaag tagtcctgct tgtcgttgaa gtacttgacc gggttcccgt
      361 cgatatccag ggcctggatt ctcttgttca gcgtttcaag aaccagcagc acgccttccg
      421 ccgtgaacgc aatggccttc ggtccgtaca tcagtccttc cctggtgcct tcgccgccaa
      481 gcagcctggc ccacggcgcc gcatcgtccg aagatccctg ggccggaatc gtcagaattt
      541 ccagcttgct gtttgaccag ttcgcgccaa ccacgtaccc ggcgtcattg atcgcgatgt
      601 cgtcaagcgg ttgcgtgaat cgcccccagc tcagcccgga tgagctcatc ggcgtcgatt
      661 cgtcggcaac cacgcgtcgc aggtggtagt tgccgttcct ggtgtccagg aagaaattca
      721 tgccggtgcc gttttccgga cccatgagtt catagacgat ggtcgtcttt tccgacattc
      781 cgcagtcaag cgtcttgtag ccgatgttcg gatcggcctt cgccgacatg ttctggacga
      841 aatgaagctg catgtcggtc gtgccggtgt cgcactgggc catgttcgga ccggcggcct
      901 gccaggagta cccgaacatg ccggcgcgct ggctgaaggt gatgttgtcg agcgaacaca
      961 ggtgggaacc gacgttggcg cacgaaaggc tggaaagcgt ctcggtcggc gcgtcgcccg
     1021 cgacccactg atactttccg ctggcgtaat tcagcttctg tttgtggtga tagttcgtcg
     1081 tctgatccag tggaaccttg atctcctcaa gggtgatatc gagcttcagc ttgtcctgtc
     1141 cggaaggaac caggttcgcg acccttcccg tgcttccgcg gccggcaagc cagttgttct
     1201 gtgcgaagaa gccaacggtc acctcgacgg tgccgccggc cggaacgtcc tcgaactcca
     1261 cgacaatcgg gtctgatacg gtcgttcccg gaagggctct catcggaaga atccggtgct
     1321 ttttgtcggc ataggtggcg atgacctggt acttggtggc cgttgccggg aactgatagt
     1381 cgtccgggtc atggaagatg gtgacctgca tgtccatcgt gggcgttacg ccgttcttga
     1441 tcacccacgg gctgctggtc acttccgccg ttgtctgggc gaccgcggac agcgtcgccg
     1501 aaacgctgag cattctcaaa ccccagccga cgaaaggaat ggccccttca agttcagcct
     1561 cggtcagctt ctttgtcacg taagtggcca gcttggagca cgcctttgac aaaatcaggt
     1621 tgcccaccag cgtcgcgctg cgcgacagga cggccttcgg gcttccgccc aggccgattt
     1681 cggccgcgtt ctcgcccgcc aggaggaagc cggcgaccgc gatgatcgcg aagaccaggt
     1741 cgtcatccgc cataagatcc ttgtaccagg acgtgttttc gatgcccacg ccggcggcca
     1801 ggaagaacag cggaatgccg tactcgaaaa cgaacgtcat gatcatcccg ttgtttgcaa
     1861 ccttgggata gtcgagcttg ccggtgccaa
//
LOCUS       DAOPPA010000558         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_66600_length_1877_cov_3.133919, whole genome shotgun sequence.
ACCESSION   DAOPPA010000558 DAOPPA010000000
VERSION     DAOPPA010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66600_length_1877_cov_3.133919"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKH54_13130"
     CDS             <1..658
                     /locus_tag="PKH54_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOD00884.1"
                     /translation="DGPKPVIPRGDLRDGGGDEPAVRPCKIRVKLADGKERHIQHMMQ
                     TTFWHPDGTPMSARQFLEMLYEQIPNFFTEESELRRIWGDPTTRRKLLDGLADKGVGM
                     RELEAMQKLVDAENSDIFDVLAYVAWALPPLTRQERADRARAMISANFDTNQQAFLSF
                     VLAHYVNEGVDELDLDKLAPLLNLKYHSLPEAFAELGEAQSIRRVFAGFQRYLYQGAA
                     "
     gene            1039..>1877
                     /locus_tag="PKH54_13135"
     CDS             1039..>1877
                     /locus_tag="PKH54_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOD00885.1"
                     /translation="MRPTRLVVTVILTAIALSTASCAASRFIGYASKPDAPLPAGQVQ
                     HPALQAPVKVTFDAQHVPHIVAENEHDLWFALGYIQARDRLFQMDMLRRMAAGRTSEL
                     LGKQDAPEGMPFSDTIGMDRFFRVMGLAKDAERTIKRISKESQDIVDPYIAGINAFIK
                     SADPVPIEYRLIDQAPMPWTAADAMVVARLTSFQLSVNMAHELLRYLLQVELGPDAQK
                     EIFPSDPPIGPNIIEREDVDFRSMPRQSTPATAARLDAVMAESGTDLAAWAPTARAFL
                     GLLA"
BASE COUNT          404 a          587 c          542 g          344 t
ORIGIN      
        1 ggacggtccg aaaccggtga tcccgcgggg cgacctgcgg gacggtggcg gcgacgaacc
       61 ggcggtcagg ccgtgcaaga tccgggtcaa gctggccgac ggcaaggaac gccacatcca
      121 gcacatgatg cagacgacct tctggcatcc ggacgggacg ccgatgtcgg ccaggcagtt
      181 cctggaaatg ctgtacgagc agataccgaa ttttttcacg gaagaatccg aactgcgccg
      241 catctggggt gacccgacaa cccggcgcaa acttctggac ggactggcgg acaagggcgt
      301 cggcatgcgc gaacttgaag cgatgcaaaa gctggtggac gcggaaaaca gcgacatttt
      361 cgacgtactg gcttatgttg cctgggccct gccccccttg acccgccagg aacgcgccga
      421 ccgcgcccgc gcaatgatca gcgccaactt cgacaccaac cagcaggctt tcctgtcatt
      481 cgtcctggcc cactacgtca acgaaggcgt ggacgaactg gatttggaca aacttgcccc
      541 gctgctgaac ctgaaatacc actcgctgcc cgaggccttc gctgaactgg gcgaggccca
      601 aagcatccgc cgggtcttcg ccggcttcca gaggtacctg taccagggcg cggcgtgatg
      661 gggtgggttc aggatcccgg cgatcaacga cccggattca gcggattatc tgcatagaaa
      721 cgatgcaatc gtcgtgaaag acgttggcga acccaggccc cggttacaga acaaaatcct
      781 gccagttgtc gcgattgaca acctggaagg aaccaccggg aaccatattc cgccagtcgc
      841 gaggggcctt gggatccttg ccgccccact tgaattcaaa acctgacagc gcaccatcga
      901 cctcctgaac gagatcgatt tcggcgcgcg acggtcatca cgccacgacg cacaccagac
      961 cgaatatgaa accatgtaaa aatcccctga tgctttgcgt caaactgcgg tatcttaccg
     1021 gcatcatgga ggttccctat gcgaccgact cgacttgttg tgaccgtcat ccttactgcc
     1081 atcgcgcttt cgacagcttc gtgcgccgcg tcacggttca tcggatacgc gtcaaagcct
     1141 gacgcaccgc ttccggcggg ccaggttcag caccctgccc ttcaggcgcc ggtcaaggta
     1201 accttcgacg cacagcacgt cccgcacatc gttgccgaaa acgaacacga cctgtggttc
     1261 gcgcttggtt atatccaggc acgcgacaga ctgttccaga tggacatgct gcgcaggatg
     1321 gccgccggtc gtaccagcga acttcttgga aagcaggatg cacccgaagg aatgcctttt
     1381 tccgacacca tcgggatgga tcgtttcttc cgcgtgatgg ggcttgccaa ggatgcggaa
     1441 aggaccatca agaggatatc gaaggaaagc caggacatcg ttgatcccta tatcgcaggc
     1501 atcaacgcct tcatcaagtc ggcggaccca gttccaatcg aataccggct gattgaccag
     1561 gctccaatgc catggacggc cgccgatgcc atggtcgtgg cacgactgac cagtttccag
     1621 ctttcggtca acatggcgca cgaactgctt cgttacctgc tgcaggtcga actgggcccg
     1681 gacgcgcaga aggaaatttt cccgtcggat ccgccgattg gcccgaacat catcgagcgc
     1741 gaggacgtcg atttccgttc gatgccccgc cagtcaacgc cggcgacggc agcgcgcctg
     1801 gatgccgtga tggctgaatc cggcaccgat ctggccgcgt gggcgccgac agcgcgcgcc
     1861 ttccttggtc tgcttgc
//
LOCUS       DAOPPA010000559         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_66724_length_1874_cov_4.666850, whole genome shotgun sequence.
ACCESSION   DAOPPA010000559 DAOPPA010000000
VERSION     DAOPPA010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66724_length_1874_cov_4.666850"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1874
                     /locus_tag="PKH54_13140"
     CDS             <1..>1874
                     /locus_tag="PKH54_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOD00886.1"
                     /translation="IEETGATFTSIDGRMEISNLQPGKYTLYIPAGEFDEIRQEFKVG
                     ADDRAKPPESPIYLKRAYGAVSNQTIIRAPREARFAAKQSLSAGELVSFPGSAGDVLK
                     VVENLPGIARSSFGVGQLVVYGSPPNDTKVFINQMPFVLLYHFAGLYSVINSDFIEAI
                     DFVPAGFDASYGGAVGGIVNVKLKDEPLKAWHGSIDVNLLHAGIIVGGPYKKDSGDIQ
                     IAFRRSYYDAILKSINAFGDGRILTSAPAYYDYQVRLQHRFSGGHKLTFFINGSDDRG
                     ELINEENDVNPTFVGSVSGALWGHALQATWNWEPRAGLSNELKVQGLITQAGFDVFGQ
                     VKMKVRDHPVTIKETLTVQAHSKVVVRAGLNFDVDPYSFLLKGPQMGLDGENMTPYES
                     SEVLELSGDGVMATISPWISVEYKPVERLTLVPSIRADLNVGTWKAWSIDPRLSMTVD
                     AIKDKLSFRAAGGLYSQPPQSFTLIEGYGNPDIDPENGTHALVGFQYNPIDRLTLDFN
                     GFFKYFWNRAQVSDDATVRYDNGGIGRAWGFDVLLRMNPSERIPLTGWIAYTFVKSEL
                     YDFGLARWRPSDFQQNHNLNIAISYKLPRNWTIGGRFRLTSGYPYTKYESYQYDADND
                     "
BASE COUNT          405 a          563 c          559 g          347 t
ORIGIN      
        1 catcgaggaa accggggcga cgttcacatc gatcgacggc cgcatggaga tttcaaacct
       61 tcagccaggc aagtacaccc tttacattcc ggccggcgag ttcgacgaaa tccgccagga
      121 attcaaggtt ggcgcggacg accgggcaaa accgccggag tcgcccattt acctgaagcg
      181 cgcttacggg gcggtttcca accagacgat catcagggca ccgcgcgagg ccaggttcgc
      241 ggccaagcaa tcgctttcgg ccggggaact ggtgtccttc ccgggatccg ccggcgacgt
      301 cctgaaagtg gttgaaaacc ttcccgggat cgcccggtcc agcttcggcg tcgggcagct
      361 tgtcgtttac ggcagcccgc caaacgacac caaggtcttc atcaaccaga tgcccttcgt
      421 gctcctgtac cacttcgcgg gcctttattc ggtgatcaat tcggacttca tcgaggcgat
      481 cgacttcgtc ccggccggat tcgacgcatc ctacggcggc gcggtgggcg gcattgtcaa
      541 cgtgaaactg aaggacgagc ccctgaaggc atggcacggc tcgatcgatg tgaacctgct
      601 tcacgcggga atcatcgtcg gcggccccta taagaaggac agcggcgaca tccagatcgc
      661 gttcaggcga agctattacg acgcgatcct gaaaagcatc aatgcattcg gcgacggccg
      721 catccttacg tccgccccgg cgtactacga ttaccaggta aggctccagc accggttctc
      781 cggcggacac aagctgacgt tcttcatcaa cggttccgac gatcgcggag aactgatcaa
      841 cgaagaaaac gacgtgaacc cgacctttgt cggcagcgtt tccggagccc tgtggggaca
      901 tgcgctgcag gccacatgga actgggaacc aagggccggg ctttcgaacg agctgaaggt
      961 ccagggcctg attacccagg ccggcttcga cgtgttcggc caggtcaaga tgaaggtgcg
     1021 cgaccatccg gtcacgatca aggaaaccct tacggtacaa gcccacagca aggtggtcgt
     1081 gcgcgccgga ctgaacttcg acgtcgatcc gtacagcttc ctgctgaagg ggccgcaaat
     1141 gggactggac ggcgaaaaca tgacgccgta cgagtcgtcg gaagttctgg aactttccgg
     1201 tgacggagtg atggcgacga tttcgccgtg gatttcggtc gaatacaagc cggttgaacg
     1261 gctgacgctg gtgccttcga ttcgtgccga cttgaacgtc gggacgtgga aggcgtggtc
     1321 aatcgacccg cgactttcca tgacggtcga tgcgatcaag gacaaactca gcttccgcgc
     1381 cgcgggcggt ctttacagcc agccgccgca gtcgtttacg ctgatcgagg gatacggcaa
     1441 cccggacatc gatccggaaa acgggactca cgcgcttgtc ggcttccagt acaacccgat
     1501 cgaccgcctg accctggatt tcaacggatt cttcaagtac ttctggaacc gcgcacaggt
     1561 gtcggacgac gcgaccgtcc gttacgacaa cggcggaatc gggcgggcct ggggtttcga
     1621 cgtgctgctt cggatgaacc cgagcgaacg catcccgctg accggctgga ttgcatatac
     1681 gttcgtcaag tcggaacttt atgacttcgg cctggcgagg tggcggccgt cggacttcca
     1741 gcagaaccac aacctgaaca tcgccatcag ctacaagctg ccgcgcaact ggacgatcgg
     1801 cggtcgcttc aggctgacgt ccgggtatcc atacacgaag tacgaaagtt accagtacga
     1861 tgccgacaac gaca
//
LOCUS       DAOPPA010000560         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_66735_length_1874_cov_3.255085, whole genome shotgun sequence.
ACCESSION   DAOPPA010000560 DAOPPA010000000
VERSION     DAOPPA010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66735_length_1874_cov_3.255085"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKH54_13145"
     CDS             complement(<1..213)
                     /locus_tag="PKH54_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00887.1"
                     /translation="MVVRQGRFGRLGRSFGVSRHQAEQRTDVLYPKKFRRGCGLSSGV
                     GGYGNESVPCDQLQDVVGVFVQEAAAN"
     gene            276..1682
                     /locus_tag="PKH54_13150"
     CDS             276..1682
                     /locus_tag="PKH54_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00888.1"
                     /translation="MAFFNRKVELRFLADQARNDRAALTVLYGRRRTGKTSLLRHFAQ
                     GRRAAFFVADQGSAADQLATFSRVLFDALGETALAGTTFQSWEAGLRFAGNRAKSEPL
                     LLVLDEFGYLCQSDPSLPSVLQRLWDSDLRETRLHLVLCGSYVSFMEREVLGAKNPLY
                     GRRTGEWLLLPFRFNDTREFFPTATPDDMVTVWSILGGIPAYLERFDQASGIGQNILD
                     SILTRGTPLYNEPRFLMMEELRDPHIYFSICHAVAFGRTTPNEIAQAAGLTDRTVAGR
                     YMDTLREMRILERMTPVTERNPERTRRSRYRLADPFFRFWFRFVLPNHSALESGDPVH
                     VLESKVLPHLDKHVSIGFEDICRQHVQDLGRAGALPARYDRVGSWWRPNAEVDVVAIA
                     DEGSLLMGECKWSINPVGTDVLDSLMARREGIEADLGHKPSTTTFALWSRSGFTPALK
                     RRAAAEGILTFDLTDIVP"
BASE COUNT          391 a          579 c          508 g          396 t
ORIGIN      
        1 attggccgcc gcttcctgga cgaacacgcc cacgacatcc tgaagctgat cgcacgggac
       61 tgactcgtta ccatacccac ccacgcctga actcaaaccg cacccacgcc tgaacttttt
      121 cgggtacaac acatccgtcc gctgctccgc ctgatgcctt gagacgccga agcttcggcc
      181 aaggcgcccg aatcgcccct gccgcacgac catctgggac ttccattttg tataatcttg
      241 attatactaa tcgtaattag acaaaggagg cttacatggc tttcttcaat cgaaaagtgg
      301 agttgcgttt tctggcagat caggctcgca atgaccgtgc tgccttaact gtgctgtatg
      361 gccgcagaag aaccggcaaa acatccctgc tgcgccattt cgcgcagggg cgccgcgcgg
      421 cctttttcgt tgctgaccag ggctcggccg ccgaccagct cgcgacgttt tcacgcgttc
      481 tgtttgacgc gctcggcgaa accgcccttg cgggaacgac attccaatcc tgggaagcgg
      541 gactgcgctt cgccgggaac cgcgcgaaaa gtgaaccact tctgcttgtc cttgatgaat
      601 tcggatacct ttgccagtcc gatccttcac ttccatcggt tctgcaaaga ctctgggatt
      661 ctgacctcag ggagacgcga cttcacctgg tgctttgcgg gtcttacgtg tcattcatgg
      721 aacgcgaggt tcttggcgca aagaatccgc tttatgggcg acggaccggc gagtggctgc
      781 ttctgccttt ccgcttcaac gacacgcgcg aattcttccc gacagccact cccgacgaca
      841 tggtgactgt ctggtcaatc ctgggcggaa tccccgcata cctggaacgg ttcgaccagg
      901 cttccggaat cggccaaaat atcctggaca gcatcctgac ccgcggaacc ccgctctaca
      961 acgaaccaag attcctgatg atggaggaac tgcgcgatcc gcacatctac ttttccatct
     1021 gccacgccgt ggcttttgga aggaccaccc ccaatgagat tgcccaggcc gctggactta
     1081 ccgatcgaac cgtcgccgga cgctacatgg acacattgcg cgaaatgcgg atcctggaac
     1141 gaatgacgcc agtcactgaa cgcaatccag aaagaacgcg acgcagtcgt taccgccttg
     1201 ccgatccatt cttcaggttc tggttcaggt tcgtgctgcc caaccacagc gccctggagt
     1261 ccggcgaccc cgtacatgtt ctggaatcaa aggtgctgcc gcacctggac aagcacgtct
     1321 caatcggttt cgaggatatc tgccgccagc atgtccagga tctgggccgc gcgggagccc
     1381 tgcccgcccg ctatgaccgc gtcggatcgt ggtggcgtcc gaatgccgaa gtcgatgtag
     1441 tcgcgattgc cgacgaaggc tccctgctga tgggcgaatg caagtggagc atcaatcccg
     1501 tcggaaccga cgtcctggac tctctgatgg cccgtcggga aggcatcgaa gccgaccttg
     1561 ggcacaagcc gtcgacgact acattcgccc tgtggtcgcg atcgggcttc acaccagccc
     1621 tgaagcgccg ggcagccgca gaaggaatcc tgacattcga tctgaccgat atcgtcccgt
     1681 gatgcagcca atggtttcgg ccttcgacaa ttgcccccat gtcgctcaca gacggacagt
     1741 cccagtcttt ggcccgatgg tgccggccag gggcatgtga atggacgaag tcattgcaat
     1801 cacgagttcc tggcaacgat tcttgaaatc aaagaggcaa taagggggtg gcgatcatta
     1861 tgaattgggg ttgg
//
LOCUS       DAOPPA010000561         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_66928_length_1870_cov_3.911846, whole genome shotgun sequence.
ACCESSION   DAOPPA010000561 DAOPPA010000000
VERSION     DAOPPA010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66928_length_1870_cov_3.911846"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKH54_13155"
     CDS             complement(<1..399)
                     /locus_tag="PKH54_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00889.1"
                     /translation="MRSRTTLAAAFLAACAMVAGCGDEPVKPVMADIVPHVRIAASDT
                     LHEAFPWPIEAQDGPATIRDGNPGTSWKVPAGQTVLTLDIQPWLQRPVELGSLSIQAS
                     GGVDSVSVALMGACGSTATVVEWPDISVALD"
     gene            complement(413..1432)
                     /locus_tag="PKH54_13160"
     CDS             complement(413..1432)
                     /locus_tag="PKH54_13160"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD00890.1"
                     /translation="MENRKQAVPTYGEKLLSTLDASYFDNCGVICAQEAWDLVKDEFP
                     ATPVVVMVPETMEQSLLEAQIRRAPGGVKTWFGIGGGSACDAAKMAVWLLGGRLILMP
                     SIVSVDAAFTAAVGVRVDHHVRYVGMVYPDYLLIDFDLLRRSPKRLNIAGIGDILSIF
                     TALPDWLLGRDVTGEKYSDQMAAESAALLARLMAGAVEIRDCTNLGLKLISDLYYAEV
                     ALCDAHGNSRPEEGSEHYFAYCLESLTHRPYIHGELISMAVVLTGIYQGQDVEPVVRF
                     LDQAGVCWRPDQVEVTRAQIVATLLALPEYLKGETHLPYGIYHHVPTTQERVDDLLVK
                     FYSYV"
     gene            complement(1480..>1870)
                     /locus_tag="PKH54_13165"
     CDS             complement(1480..>1870)
                     /locus_tag="PKH54_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00891.1"
                     /translation="GFMGCVDVPVSVVKFGELLTWVNVPGELTPELVLGVYPDPEPVP
                     GEFADAEREPYLEQFIDTPNRFVACLSDTEIGYIYPRYQEDRPNHEHQRNSASPNVAG
                     TLMAGYAALFEDIAASEAAFFGEDSAE"
BASE COUNT          374 a          611 c          555 g          330 t
ORIGIN      
        1 gtccagcgcg accgagatgt ccggccactc gaccacagtg gccgtggatc cgcaggcacc
       61 catcagcgcc accgaaaccg aatccacgcc tccggacgcc tggattgaca gtgaacccag
      121 ttccaccggg cgctgaagcc acggctggat gtccagcgtc agcaccgtct gccccgcagg
      181 aaccttccag gaggtgccgg gattgccgtc acggatggtt gccggaccgt cctgcgcctc
      241 gatcggccag gggaatgcct cgtgcagcgt atcgcttgcg gcaatgcgga cgtgcggaac
      301 gatatctgcc atgacaggct tgactggctc gtccccgcat cccgcaacca tcgcacaggc
      361 ggccagaaag gccgccgcca gtgtcgtcct gctgcgcatc ggcaccccgc tgtcacacgt
      421 aggagtaaaa cttgaccagc agatcatcga cgcgctcctg cgtcgttgga acatgatgat
      481 aaatcccgta cggcagatgt gtctcgccct tcagatattc gggcagcgcc agcagggtcg
      541 ccacaatctg ggcgcgggtc acctcgacct gatccgggcg ccagcagacg cccgcctgat
      601 ccaggaaccg aaccaccggt tcaacgtcct gcccctggta aatcccggtc agaaccaccg
      661 ccattgaaat gagctcgcca tggatgtagg gacgatgggt cagcgattcc aggcagtacg
      721 caaagtagtg ctcggatccc tcctcggggc gactgttgcc gtgtgcgtca caaagcgcga
      781 cctcggcgta gtaaagatcc gaaatcagct tcaggcccag gttcgtgcaa tcgcgaattt
      841 ccacggcgcc ggccatcagc cgggcaagca gcgctgccga ctcagcagcc atctggtcgc
      901 tgtacttttc gcctgtcaca tcacgaccca gcagccaatc cggcagggcc gtgaaaatcg
      961 acaggatgtc gccgatgccg gcgatattca gacgcttcgg cgacctgcgc agcaggtcga
     1021 aatcgatcag caggtagtcg ggataaacca tcccgacgta cctgacgtga tggtcaacac
     1081 gcacaccgac ggcggcagtg aaggccgcat cgacgctgac gatcgaaggc atcaggatca
     1141 ggcggccgcc aagcagccag acggccatct tggcagcgtc gcatgccgat ccgccaccga
     1201 ttccgaacca ggtctttacg ccccccggcg cccgcctgat ctgcgcttcc agcagggact
     1261 gctccatcgt ctcgggaacc atcacgacca cgggcgtggc gggaaactcg tccttgacca
     1321 gatcccatgc ttcctgggcg cagatgacgc cgcagttgtc gaaataactt gcgtcaagtg
     1381 tcgatagaag cttttccccg taagtcggta ccgcctgttt cctgttctcc atctcgcttc
     1441 tccgttcccg agcggacaag gccggtgcgg gaatcttcgc tattcggcag agtcttcacc
     1501 aaagaatgcg gcctcgctgg ccgcaatgtc ctcaaaaagg gcggcgtaac cggccatcag
     1561 ggtgccggcg acgttggggc ttgccgaatt gcgctgatgc tcgtggttcg gcctgtcctc
     1621 ctgatacctt ggatagatgt agccaatctc cgtgtcggaa aggcaggcga cgaagcggtt
     1681 cggcgtgtcg atgaactgct cgaggtaagg ctcgcgctcg gcgtcggcga actcgcccgg
     1741 caccggctcg gggtcgggat agacccccag gaccagttcc ggggtcaact cgccggggac
     1801 gttgacccac gtaagcagtt cgccgaactt gacgacgctt acgggaacat cgacgcagcc
     1861 catgaagccg
//
LOCUS       DAOPPA010000562         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_67168_length_1866_cov_2.845941, whole genome shotgun sequence.
ACCESSION   DAOPPA010000562 DAOPPA010000000
VERSION     DAOPPA010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67168_length_1866_cov_2.845941"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PKH54_13170"
     CDS             complement(<1..1831)
                     /locus_tag="PKH54_13170"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOD00892.1"
                     /translation="MIRNRLIKAPVIRRLFAPVAVVWALSFSTALVFVSRVASSAEPD
                     LVEMAIMSDGVELKTAVHMPDPGVFSPPWPTVLLRTPYPEILGQLDPSIADMVIHYMV
                     DMFGIALVVQDTRGTGGSGGEPDAFFTDWRDGPETVEWLACQDWCNGQVMQFGLSALA
                     IPGYLSAPGAPDEMKCQYLGEAAPDMYSMAAFHGGVFKKSTVESWLSWVGASSRLSDL
                     SNHRNCDNFWDTIILDRIPGDIHTAAMHIGGWYDVFTEGTIAGFRAYRDRGDEWARNN
                     QYMVIGPWDHWELAQSLGVTESNGYAMQSDMLAWVSYCLAGDDSTVRKWPHVRYYVMG
                     PDVEGAPGNEWRSAPDWPLPTSHVSFPAAFDGTLQVPESLMSHSEGADGGVEGGGHRF
                     ENNGDVEDDAGSKQAVLPAVLPFSHASPSPVVGGRNLVGSSGRRELSKVLERDDWVMF
                     TTAPLEVPFESIGRMSLKLSASTRFKDADIVARLADVYPDGSTWLISDGAIRLSRREG
                     CAALADVVPATAYDVEIDLLTSAVVFDTGHRIAVILTTTMYPKYELNPELEAESRTSG
                     PIGEALTIHGLELVMPVAGEIVEPPEQVETADSAIFVESSDEGP"
BASE COUNT          421 a          590 c          496 g          359 t
ORIGIN      
        1 cgggaccttc atccgaggat tcgacaaaaa ttgccgaatc tgccgtttcc acctgctcgg
       61 gcggttcaac aatttccccc gccaccggca tgacgagttc caaaccgtga atcgtcaacg
      121 cttcgccaat cggcccgctt gtcctggact cggcttccag ttcaggattg agttcgtatt
      181 tgggatacat ggtggtcgtc aggatgaccg cgatcctgtg tccagtgtca aacaccacgg
      241 ctgatgtaag caggtcgatt tcgacgtcat acgcagtggc tggaaccaca tcggccaaag
      301 ccgcgcagcc ctcccttctg gaaagccgta tcgcgccatc agatatcagc catgttgaac
      361 cgtccggata gacgtcggcc agcctggcga cgatgtcggc atccttgaac ctggtcgatg
      421 ccgacagctt cagggacatg cgaccaatcg attcaaacgg aacttcaagc ggggcggtcg
      481 tgaacatgac ccagtcgtcg cgctcaagca ccttggaaag ttccctccgc ccggaagagc
      541 cgacaagatt ccgcccgcca acaacgggtg acggactggc gtgggaaaag ggaagaaccg
      601 cgggcagcac ggcctgtttt gaaccggcat catcttccac atcgccgtta ttttcaaagc
      661 gatgtccacc accttcgaca cctccatcgg caccttccga atgactcatc aaactttcgg
      721 gaacctgaag cgttccgtcg aacgcggccg ggaacgaaac atggctggtc ggcaacggcc
      781 agtccggggc gcttcgccat tcgttgccag gcgccccttc cacgtccggc cccatgacgt
      841 aatacctgac atgcggccac ttcctgaccg ttgaatcatc gccggccagg cagtatgaaa
      901 cccaggccag catatcggac tgcatggcgt atccgttcga ttccgtgacg ccaagcgact
      961 gggccagttc ccagtgatcc catgggccga tcaccatgta ctggttgttc cttgcccatt
     1021 catcgccacg atccctgtaa gccctgaatc cggcgatggt tccttcggta aaaacgtcgt
     1081 accagccgcc gatatgcatc gcggccgtgt ggatgtcacc cggaatcctg tcaagaatga
     1141 tcgtgtccca gaaattgtcg cagttgcggt gattcgaaag atcggaaagc ctggatgaag
     1201 ctccgaccca cgacagccag ctttcaaccg tgctcttctt gaagaccccg ccatgaaaag
     1261 cggccatcga atacatgtcc ggcgccgctt cgcccagata ctgacacttc atttcatccg
     1321 gagccccggg ggccgacaga tagcctggga ttgccagggc ggaaagcccg aactgcatca
     1381 cctgtccgtt gcaccaatcc tgacaagcaa gccattcgac cgtttcgggc ccatcccgcc
     1441 agtcggtgaa aaaggcatcc ggttcgcccc cggaaccacc tgttccgcgg gtatcctgta
     1501 caacaagggc gattccgaac atatcgacca tgtaatggat caccatgtcc gcaatcgacg
     1561 gatcaagctg cccgagaatc tcggggtacg gagtccgcaa cagcacggtg ggccacggcg
     1621 gggaaaaaac gccgggatcc ggcatgtgta cggcggtctt cagttccacg ccatccgaca
     1681 tgatcgccat ctcgaccaga tcaggctcgg ctgatgacgc aaccctggat acgaacacaa
     1741 gcgcggttga aaacgacagg gcccagacga cagccacggg cgcgaataac cttctgatga
     1801 cgggcgcctt gattaaccga ttgcgaatca tcactcctcc ggaattgacc gcccagacat
     1861 gacgcg
//
LOCUS       DAOPPA010000563         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_67487_length_1859_cov_4.263304, whole genome shotgun sequence.
ACCESSION   DAOPPA010000563 DAOPPA010000000
VERSION     DAOPPA010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67487_length_1859_cov_4.263304"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PKH54_13175"
     CDS             complement(<1..1431)
                     /locus_tag="PKH54_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOD00893.1"
                     /translation="MARYVELRAASAFSFLSAASVPEDLADQAARLGCDSMALADRDG
                     VSGIPRFIKACRAGGIRPICGARISFDPGTPFLTRGGRLPQLLLLCRDGEGWGNLCQM
                     ITMAHSGREKDMPVAGWDLLEELHHGLVCLAGSMDGPVTSIAATGERTSAASATARLA
                     SIFGRDSLFLDLQRHGDPVQESANRFLADIAGGVGIRTIATNDVRYADAAASRVFDVL
                     QAIRNHVTLDAAATILPAGHRHLQRDHSTMESLFYDMPGIIDRTGELAESLRFDPADI
                     EYRFPEYPVTAPTTPDSLLCQLVEAGLTRRYSSVTQGVRGQVEHELQIIGKLGLAGYF
                     LLVQDIVGFCGRNGILAQGRGSAANSVVCYALGITAIDPVGMGLLFERFLSEERGEWP
                     DIDLDLPSGEQREMVIQHVYEKYGVSCVGMTSAVSTFRGRSTIREAGAAFGLDPDCLA
                     RLARLVRPFDFSNDQESFLNKVSQAGL"
     gene            complement(1440..>1859)
                     /locus_tag="PKH54_13180"
     CDS             complement(1440..>1859)
                     /locus_tag="PKH54_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00894.1"
                     /translation="DLTGRLAAIVGEGNVGSPDPDARPARPEYAPVPFDPCPSDARHT
                     RPVRRPGSPAMAFRRFNPPYKATVATGNDLPAEISSNLVSGRICRVAGPWYLDASWWG
                     DGPVAGALYDIEIPGRGVFRLWHDLLDDGWLIDGVWD"
BASE COUNT          361 a          581 c          593 g          324 t
ORIGIN      
        1 cagtcccgcc tgggaaacct tgttcagaaa ggactcctgg tcattagaaa agtcgaaagg
       61 acgcaccaga cgggcaaggc gcgcgagaca gtccggatca agtccgaagg cagcgccggc
      121 ctcgcgtatg gtgctgcgtc cccggaaagt ggaaaccgcg gaagtcatgc cgacacacga
      181 aaccccgtac ttttcgtaaa cgtgctggat gaccatttcc ctttgttccc cggaagggag
      241 atccaggtcg atgtccggcc attcgccccg ttcctccgac aggaaccgtt caaacagaag
      301 tcccatcccg accggatcga tcgcggtgat ccccagagcg tagcagacga ccgaattggc
      361 cgccgaacca cggccctggg caagtattcc gttacggccg cagaagccga caatgtcctg
      421 gacaagcagg aagtagccgg ccaggccgag cttgccgatt atctgaagtt catgttcgac
      481 ctggcccctg accccctgcg tgaccgaact gtagcgccgc gtgagacctg cctcgacaag
      541 ctggcacagc agcgagtccg gggttgtcgg ggccgtgacc gggtattcgg ggaacctgta
      601 ttcgatgtcg gccgggtcga atcgaagcga ttcggcaagt tccccggtgc ggtcaatgat
      661 tcccggcatg tcgtaaaaaa gggattccat ggtcgaatga tccctttgaa ggtgccggtg
      721 tcctgccggc agaatggttg ccgcggcatc gagggtgacg tggttgcgta tggcctgcag
      781 cacgtcaaaa acgcggctgg ccgcggcgtc ggcgtacctg acgtcgttcg ttgcgatcgt
      841 ccggataccg acccccccgg caatgtccgc aaggaagcgg tttgcgcttt cctgtaccgg
      901 atcgccgtgc cgctgaagat ccaggaacag cgagtcgcgt ccgaatatcg acgcaagccg
      961 tgccgtggcg gatgcggccg acgttcgttc gccggtcgcg gcgatcgatg taacgggacc
     1021 gtccatcgag ccggccaggc agaccagtcc atggtgcagc tcctcaagca gatcccaccc
     1081 ggccaccggc atgtcctttt cccgcccgga atgggccatg gttatcatct ggcagaggtt
     1141 tccccagcct tcgccatcgc ggcacagaag cagcagttgg ggaaggcggc ccccgcgggt
     1201 aagaaaaggc gttccggggt cgaaactgat tcgagcaccg cagatcggcc tgatgccacc
     1261 ggccctgcac gccttgatga agcgggggat cccggacact ccgtcgcgat cggcgagcgc
     1321 cattgagtcg cagcccagcc tggcggcctg gtcggcaaga tcttcgggta ccgacgcggc
     1381 cgacaggaat gaaaacgcgg aggccgccct gagttcgaca tatcgtgcca tctgttgcct
     1441 cagtcccaga ccccgtcgat gagccatccg tcatccagca ggtcgtgcca gagcctgaaa
     1501 acgccgcgcc ccggaatttc aatgtcgtag agcgcgcccg ccacagggcc gtcgccccac
     1561 caggacgcgt caaggtacca ggggcccgcg acccggcata tccgtccgga aaccaggttc
     1621 gacgagatct cggccggcag gtcgttccct gtcgcaaccg ttgccttata aggcgggttg
     1681 aagcgcctga atgccatggc tggcgaaccg ggacggcgaa ccggtcgcgt gtgccttgca
     1741 tcggaagggc aggggtcgaa tggaactggt gcgtattccg ggcgtgctgg ccgtgcgtcg
     1801 ggatcggggc tgccgacgtt tccttccccg acgatggcgg caaggcgtcc ggtaagatc
//
LOCUS       DAOPPA010000564         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_68321_length_1842_cov_3.639619, whole genome shotgun sequence.
ACCESSION   DAOPPA010000564 DAOPPA010000000
VERSION     DAOPPA010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68321_length_1842_cov_3.639619"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKH54_13185"
     CDS             complement(<1..230)
                     /locus_tag="PKH54_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00895.1"
                     /translation="MKSIFRTLLAVVAAVSVMSATSMASAQSGGGGGWMTQGAEILPA
                     GKYAFEARVGWPSTDLGVHIPVMSKLEVTPFLT"
     gene            678..>1842
                     /locus_tag="PKH54_13190"
     CDS             678..>1842
                     /locus_tag="PKH54_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD00896.1"
                     /translation="MRANLSILVASGLVVFLAACGTSDPVSDTREDNLPIDVDWDNGQ
                     HDSMSDETPQEDTNPDSAGELPADVPRDVASETVGDSIVDVGADASNPGCIDPDGDGY
                     GQGVDCLGPDCEEGNPNCNVNCITDSDQDGAVNCRDLCPDDNPKHEPGVCGCSVPDMD
                     SDRDGVLDCLDDCPLNPNKIRAGVCGCELPDLDPDQDDRPSCVDGCPTDPDKVEAGVC
                     GCGVADDDSDDDGLPDCLDRCPNNAAKTDPGACGCNQPDTDTDGDLRADCIDECPANA
                     GKTWPGQCGCAVADTDSDGDGTANCNDQCPNDSRKTKPGACGCGAYDTDKDLDGWADC
                     VDTCSNDPFKKVPGVCGCGVADVDADQDGTPNCVDGCPFDVAKTAPGACGCGVV"
BASE COUNT          371 a          519 c          580 g          372 t
ORIGIN      
        1 gtcaggaacg gcgtgacctc gagcttggac attacgggaa tgtgaacgcc cagatctgtt
       61 gacggccagc cgacgcgggc ctcgaatgca tacttgcccg ccggcaggat ttcggcgccc
      121 tgggtcatcc atccgccacc accgccggac tgggcggatg ccatcgaggt ggctgacata
      181 accgaaaccg ccgcgaccac tgcaagcaac gtcctgaaaa tcgacttcat cgtacatcct
      241 ccagaagaag aaaattgtta tcgcgacttt acggaaaaat gcccgcaaag ccttattctt
      301 agcgggattt tatccctgaa aacggatttt gacaaatgcc ggcctgcatt gccatgagcc
      361 ttcaaagggt taggtaagca gtaattgccg gcgcgatgtc cagggcccgg cggatagtag
      421 tcagcgtggt tcaagtcacg aacttgatcg ccaggattgg cccgtccggg atttgtaacg
      481 gccgggtaag tctgtcgtta ccgccttaat caggaattct ggccagtgcc ggttccttcg
      541 gtgcctggac gtggtggaac cgccgtacaa atcccggtgg catcccatat atctcgcaca
      601 catgcgcggg gcgttctggt acaatctgtc cgcttcgggc cctgcccgta atgtttctgc
      661 tgtcgaaggt gatgtttatg cgcgcaaatt tgtcgattct ggtcgcttcc ggattggtcg
      721 tttttcttgc cgcgtgcgga acttcggatc ctgtctcgga cacgcgggag gataacctgc
      781 ccatcgatgt tgactgggac aacggtcagc acgacagcat gtccgatgaa acgccgcagg
      841 aagacacaaa tccggattcg gccggggaat tgccggcaga tgttccgcgt gacgtcgcaa
      901 gtgaaacggt cggcgattcc atcgttgatg tcggggccga cgcttccaat cccggctgca
      961 tcgatcccga cggtgatgga tatggccagg gcgtcgattg cctggggccg gactgcgagg
     1021 aaggaaatcc caattgcaac gtcaactgca ttacggattc cgatcaggac ggcgccgtga
     1081 actgtcgcga tttgtgcccg gacgacaatc ccaagcatga acctggggtg tgcggatgtt
     1141 ccgtgccgga catggattcc gatcgcgacg gcgtgcttga ctgcctggac gactgcccct
     1201 tgaatccgaa caagatccgt gccggcgtgt gcggttgcga attgccggat ctcgatcccg
     1261 accaggacga ccggccgtcc tgcgttgatg gatgcccgac tgatcctgac aaggtggaag
     1321 ccggtgtgtg cggttgcggc gtggccgatg acgattccga tgacgacgga ttacccgatt
     1381 gcctggatcg ctgcccgaac aatgcggcaa agaccgatcc cggcgcatgt ggctgcaatc
     1441 agccggatac cgataccgat ggcgatctgc gggctgactg catcgacgag tgtccagcaa
     1501 acgcgggcaa gacttggccg ggacagtgcg ggtgcgctgt tgccgacacc gattccgacg
     1561 gcgacggcac cgccaactgc aacgaccagt gcccgaacga cagcaggaag acgaagccgg
     1621 gcgcttgcgg gtgcggtgcc tacgacacgg acaaggatct ggacggctgg gccgattgcg
     1681 tcgatacgtg cagcaacgac ccgttcaaga aggtacctgg cgtctgtggc tgcggcgtgg
     1741 cggacgttga tgcggatcag gacggaactc cgaattgtgt cgatggctgt ccctttgatg
     1801 tggccaagac ggcccccggc gcgtgcggat gcggcgtcgt tg
//
LOCUS       DAOPPA010000565         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_68326_length_1842_cov_3.341354, whole genome shotgun sequence.
ACCESSION   DAOPPA010000565 DAOPPA010000000
VERSION     DAOPPA010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68326_length_1842_cov_3.341354"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..166
                     /locus_tag="PKH54_13195"
     tRNA            80..166
                     /locus_tag="PKH54_13195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:114..116,aa:Ser,seq:cga)
     gene            245..1156
                     /gene="amrB"
                     /locus_tag="PKH54_13200"
     CDS             245..1156
                     /gene="amrB"
                     /locus_tag="PKH54_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD00897.1"
                     /translation="MTRRSVVSGLYFPESAGHLSQVLAGLGQGSSAGGASRVDGVRGV
                     VVPHASIEYCGKVAAALWSRVRVPDHVLVIGPNHTGLGAVAAVADRFSYSTPSGTFEM
                     DRDMAAAIVDACPLLAFDDLAHHEEHSIEAVLPYIQAANPSVRLTALCLRSMSYSDCA
                     RIATSIAGVVACFRDRGEEVLLVASSDLNHNEPLRIGARKDRMVLDRMVGLDAPGLYQ
                     AVVEHHITMCGVVPVVVMMLAAREAGAGYGELVGYQTSMDCTGDASSVVGYGAVMLRD
                     RCPADEFRRLDRGSGAGVVAGSCGDCR"
     gene            1153..>1842
                     /locus_tag="PKH54_13205"
     CDS             1153..>1842
                     /locus_tag="PKH54_13205"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879492.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOD00898.1"
                     /translation="MRTVEYLTGTRLSCKGWQQEAARRMLHNNLHPDVAENPEELVVY
                     GGIGKAARNWDCFEKIDRALVELEDDETLLVQSGKAVGVIRTHADAPRVLIANSNLVP
                     AYATWEKFRELDRLGLMMYGQMTAGSWIYIGTQGILQGTYETFAAAARTHFDGDLGGR
                     LVLTGGLGGMGGAQPLAITMAGGVALCVEVDRHRIQRRLDTRYLDVSTDSLDEALQMA
                     RRAMMAREPLSI"
BASE COUNT          317 a          515 c          610 g          400 t
ORIGIN      
        1 tggaaatcgt gtgaggtcaa aagcctccga gggttcgaat ccccctctct ccgcagttcc
       61 gttagtgacc ttgttcattg gagagatggc cgagtggtcg atggcacctg actcgaaatc
      121 aggcgtaggc gtcagcttac cgggggttcg actccctctc tctccgctta acagtatctt
      181 cgtccagtgg aataccgccc ggcggtaacg tatattcgcc tgcgggctct cccggagtat
      241 tccaatgacc aggcgttcgg ttgtttccgg tctgtatttt cccgagtcgg ccggccatct
      301 gtcccaggtt ctggctggac tggggcaggg cagttcggcc ggcggcgctt ccagggtgga
      361 cggcgtccgg ggtgttgtcg ttccgcacgc ttcaatcgag tattgcggca aggtggccgc
      421 ggcactctgg tccagggtca gggttcccga tcatgtcctg gtcatcgggc cgaaccatac
      481 cggtcttggc gcggtcgcgg ccgtggccga ccggttttcg tattcgacac cttcagggac
      541 gtttgaaatg gatcgcgaca tggccgcggc aatcgtcgac gcctgtccgc ttcttgcctt
      601 tgacgacctg gcccatcacg aagagcattc gattgaagcg gtgcttccat acatccaggc
      661 cgccaacccg tccgtccgtc tgaccgcgct ttgtctgcgt tcgatgtcgt attccgactg
      721 cgcgcggatt gccacatcca tcgccggggt tgtcgcctgt ttcagggatc gcggcgaaga
      781 agtccttctg gtggccagtt ccgacttgaa ccacaacgag ccattgcgca tcggtgcgcg
      841 gaaggacagg atggtgctgg atcggatggt cggacttgat gctcccggac tttaccaggc
      901 cgtcgtcgag caccacatca cgatgtgcgg ggtcgttccc gtcgtggtaa tgatgctggc
      961 ggcccgggaa gctggcgccg gatacggcga gctggtaggc taccagacga gcatggattg
     1021 caccggggac gcgtcctcgg tcgtcggata cggtgccgtc atgcttcggg atcggtgccc
     1081 cgccgacgag tttcggagac tggatcgggg aagcggcgcc ggagttgtgg ctggttcttg
     1141 tggagactgt agatgaggac tgtcgaatac ctgaccggga cgaggctttc gtgcaaggga
     1201 tggcagcagg aagccgccag gcggatgctg cacaacaacc ttcaccccga tgttgccgaa
     1261 aatccagaag aactggtcgt ttacggtgga atagggaagg ccgcccggaa ctgggactgt
     1321 tttgaaaaga ttgaccgggc gctggttgaa cttgaggatg acgaaacgct tctggtgcag
     1381 tccggcaagg ctgtgggcgt aatccgcacc cacgccgatg cgcccagggt actgattgcc
     1441 aattcgaatc tggttcctgc ttatgcgacc tgggagaagt tccgcgagtt ggatcggctg
     1501 ggtctgatga tgtacggcca gatgaccgcc gggtcatgga tttacattgg aacccagggg
     1561 attctgcagg ggacgtatga gaccttcgcc gccgcggcaa ggactcattt cgacggggat
     1621 ctcgggggcc ggcttgtcct tactggcggg ctcggcggca tgggcggcgc gcagcccctg
     1681 gccatcacga tggcgggcgg cgtggctttg tgcgtggaag tcgatcgtca ccgcattcag
     1741 agaaggctcg acaccaggta tcttgacgtt tccaccgaca gcctggacga ggcacttcag
     1801 atggcccggc gggcgatgat ggccagggag cccttgtcca tc
//
LOCUS       DAOPPA010000566         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_68708_length_1835_cov_3.456180, whole genome shotgun sequence.
ACCESSION   DAOPPA010000566 DAOPPA010000000
VERSION     DAOPPA010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68708_length_1835_cov_3.456180"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PKH54_13210"
     CDS             complement(<1..1622)
                     /locus_tag="PKH54_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00899.1"
                     /translation="MIKSLLTAMLATATFLFISGCGGSVQETDASLADTEADAMTGDD
                     TGDETDMMDVHADQGHGDSAQNDAADGADAADNGNEVFMGPVQADCRPNSQPFPTRPE
                     PIEKATLPLLHVEGRDIVDDEGNKVMLRGMNFGSWLMMESWLAGIGVLGQEELLGMMP
                     DKAVEFGVAELFGEAQAINIIDCSFQLKAAWLCVQEWRQYMEENMKPGLEAGSGAFWA
                     WFDSEPWIFEERALWNWLTKRFGYDGMNELRTTFQDNYITERDFELVAEMGMNLVRVP
                     VWYQTLETDIDGENAFVLEGWRRLDQVIEWARKYRLYIMLDLHGAPGGQSPWWHQGLE
                     NGGQFFTRQDCIDKTARLWQAMARYFKGEPHVAVLDLLNEPAGAPDHERYLAAHQAII
                     SAIRQENTEHIIMLEDGFLAMSTLAGPAEYGWENAMMSFHDYPGGKDAANHIRMMEEE
                     ITQLEKVWDRFDCPLFYGEFNVYGPNAFNSTDDPADRWQLDAMEGVLEMMNRRGVHWA
                     PWSWKYFDAPSLWGVLYPTDGAGERLDVRDND"
BASE COUNT          345 a          572 c          505 g          413 t
ORIGIN      
        1 aagtcgttgt ccctgacgtc aaggcgttct ccggcgccat cggtcgggta caggactccc
       61 cacagggaag gcgcgtcgaa gtacttccag ctccacgggg cccagtggac gccgcgcctg
      121 ttcatcattt caaggacgcc ttccatggcg tcaagttgcc acctgtccgc cggatcgtcg
      181 gtcgagttga aggcgtttgg cccatatacg ttgaattcgc cgtaaaacag tggacagtcg
      241 aagcgatccc agaccttctc aagttgcgtg atctcctctt ccatcatccg gatatggttt
      301 gccgcatcct ttccgcccgg atagtcgtga aacgacatca ttgcgttttc ccagccgtac
      361 tcagccggcc cggccagtgt tgacatggcc aggaagccgt cttcaagcat gatgatgtgc
      421 tcggtgtttt cctgccggat tgcggaaatg attgcctggt gggcggccag gtagcgttca
      481 tggtcgggtg ccccggccgg ttcgttaaga aggtcgagca ccgccacgtg gggctcgccc
      541 ttgaaatacc gcgccatcgc ctgccacaac ctggcagtct tgtcgatgca gtcctgtcgg
      601 gtgaagaact ggccgccgtt ttcaaggccc tggtgccacc acgggctctg gccgcccggc
      661 gctccgtgca gatcaagcat gatgtaaaga cggtacttcc gggcccactc gatgacctga
      721 tccagccggc gccatccttc aaggacgaaa gcgttttcac cgtcgatgtc ggtttcgagt
      781 gtctggtacc agacgggaac gcggaccagg ttcataccca tctccgcgac aagctcgaag
      841 tcccgctcgg tgatgtagtt gtcctggaag gtcgttctga gttcgttcat gccgtcgtat
      901 ccgaaacgct tcgtcagcca gttccacagg gcgcgctcct cgaaaatcca tggctccgaa
      961 tcgaaccagg cccagaatgc cccggatccg gcttcaagac ccggcttcat gttttcttcc
     1021 atgtactgtc gccattcctg gacgcacagc catgcggcct tcaactggaa actgcagtcg
     1081 atgatgttga tggcctgcgc ctcaccgaaa agttccgcca cgccgaattc gacggccttg
     1141 tccggcatca tccccagcag ttcttcctgg cccaggacac cgattccggc aagccagctt
     1201 tccatcatca gccagcttcc aaagttcatg ccgcgaagca tcaccttgtt cccttcgtca
     1261 tcgacgatgt cgcgtccttc aacatgaagc aggggaagcg tggccttctc gatcggttcc
     1321 ggtcttgtcg ggaacggctg gctgttcggc ctgcagtcgg cctggaccgg ccccatgaag
     1381 acttcattgc cgttgtccgc cgcatccgcc ccgtcagccg cgtcgttctg ggccgagtcg
     1441 ccgtgcccct ggtcggcgtg cacatccatc atgtcggtct cgtcgcccgt gtcgtccccc
     1501 gtcatcgcgt ccgcttcggt gtcggcaagg gatgcatcgg tttcctggac cgagccgccg
     1561 cacccggaaa tgaaaaggaa cgtcgcggtt gcaagcatcg ccgtcagtaa gctcttaatc
     1621 attcaagacc ccttcgaaga ttcactgaaa cgaaaaagtg ggtgaggtac ttcaaagtca
     1681 cccgctgtat tttgacagta tatgtcatat tatcgggctt gaggcaatcg agtgcgtcgg
     1741 caccaaggaa ggacaacatg aagctgcttg aatcactggc acttgccgcc atcctcgccg
     1801 ttggctgcgg gaatagtccg tccgatttcg acaat
//
LOCUS       DAOPPA010000567         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_68948_length_1831_cov_4.064752, whole genome shotgun sequence.
ACCESSION   DAOPPA010000567 DAOPPA010000000
VERSION     DAOPPA010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68948_length_1831_cov_4.064752"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PKH54_13215"
     CDS             complement(<1..1026)
                     /locus_tag="PKH54_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00900.1"
                     /translation="MQAMRSPADTMPIMPYTRAMNDAASQVSVERRAKKHVKAPVHRW
                     FAVCSPGTEAIVRAELATLGLEPAAVDIHGGVEFPGRLDDGMRANLHLRTAARILLRV
                     ASFRARAVEDVFRETVAQPWEAFLPQGCRLQIASTIHGSRLNSRSLLESTIREAVCRR
                     FADQGLAVPLPESAVAADTTRNTKTDAAALTAAGAQAIQARLDSDHIELSLDSSGELL
                     HRRGWRREAVAAPIRENLAAAILLAAGFAPGMNLVDAMCGSGTFAIEAAHIATGTPPG
                     ISATPPRTFAFMEWPAFSRPAWDHLCKLAGPVPTDSEIVARDIDPAVLQAASTNADLA
                     GATSMITF"
     gene            1199..>1831
                     /locus_tag="PKH54_13220"
     CDS             1199..>1831
                     /locus_tag="PKH54_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD00901.1"
                     /translation="MGDSGAINSKRLTRWQVQTFATLWGGYASYYLCRMNFSVAQPMI
                     LKDFPDWSSAEIGLIPTVYAFAYAIGQFVNGQLGEKFGARKMMTLAMSMAVITNLLFS
                     QVSSLPLMLLLWAVNGYAQSAGWSLVIKTLTNWTTTRHRGLLVGLISTCYQVGNVASW
                     LLAGYVAGTYGWRMAFMVPSIILAAMTVVLAVALRDDPRLVGFGRVRDDLG"
BASE COUNT          330 a          523 c          614 g          364 t
ORIGIN      
        1 gaatgtgatc atcgacgtgg caccggcaag gtcggcgttg gtcgaggcgg cctgcaggac
       61 cgccgggtcg atgtcgcggg cgacaatctc ggagtccgtc gggaccggcc cggcaagctt
      121 gcacaggtga tcccaggccg ggcgtgaaaa ggccggccat tccatgaagg caaaggtgcg
      181 gggcggggtc gccgagatgc cgggtggcgt accggtcgcg atatgggcgg cctcgatggc
      241 aaatgtgccg gatccgcaca tggcgtccac caggttcatc cctggcgcga agccggcggc
      301 cagcaggatt gcggcggcca ggttttcgcg gatcggcgcg gcaacggctt cgcggcgcca
      361 gcctcggcgg tgaagcagtt ccccggacga atccagtgaa agttcgatat ggtcggagtc
      421 caggcgggcc tgtattgcct gcgcgcccgc ggccgtgagt gccgcagcat ctgtctttgt
      481 gttcctggtt gtgtcggcag ccaccgcgga ttccggcagc gggacggcaa gtccctgatc
      541 ggcaaagcgg cgacagaccg cttcgcggat ggtcgattcc agcagactgc ggctgttcag
      601 cctggagccg tgaatggtgc tggcaatttg aagccgacac ccctgtggca ggaacgcctc
      661 ccagggctgt gccaccgttt cacggaagac atcctcgacc gcgcgggcac ggaacgacgc
      721 gacgcgcagc aggatgcgcg cggcagtccg aaggtgaagg ttggcgcgca tgccgtcatc
      781 caggcggccc ggaaactcaa cgccaccatg tatatcgacg gcagccggct caaggccaag
      841 cgtggccagt tcagcgcgca caatcgcctc ggtgccaggc gagcagaccg cgaaccagcg
      901 gtgcaccgga gccttgacgt gtttctttgc cctgcgttcg actgaaacct gtgatgcggc
      961 atcattcatg gcgcgagtgt agggcattat cggcattgtg tcagcggggc ttcgcatcgc
     1021 ctgcatcgtc gtcctcccgc ggcgggttgt ccggaacacg cgggcgtgaa tcaataaatg
     1081 gccagaaaca atcgaaacct gtcccggccc gggcgaaaca acaattcttg cgccggaagt
     1141 gtgccaatgg cagactagtc acggcgattt ttgaccatgc ggattcgggg gaaatcagat
     1201 gggcgacagc ggggcgatca acagcaagag acttaccagg tggcaggtac agacgttcgc
     1261 gaccctttgg ggcggatacg cttcgtatta cctttgtcgc atgaactttt cggttgccca
     1321 gccgatgatc ctgaaggatt ttccggattg gtcgtcggcc gaaattggtt tgattccgac
     1381 agtttatgcc tttgcttacg caattggtca gttcgtcaac gggcaactcg gcgaaaaatt
     1441 cggcgcccgg aagatgatga cgcttgcgat gtcgatggcg gtcatcacca acctgctgtt
     1501 ttcccaggta agcagcctgc cgttgatgct gcttctgtgg gcggtcaacg gttatgcaca
     1561 gtccgccggg tggtcgctgg tcatcaagac ccttacgaac tggacgacaa ccaggcatcg
     1621 aggtctgctg gtcgggctga tatcgacgtg ttaccaggtc gggaacgtgg cgtcctggct
     1681 gcttgccggt tatgtggccg gcacatatgg atggcggatg gccttcatgg ttccgtcgat
     1741 cattcttgcc gccatgacgg tggttcttgc cgttgctttg cgtgacgacc cccggctggt
     1801 cgggttcggg cgcgttcgcg acgaccttgg g
//
LOCUS       DAOPPA010000568         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_70258_length_1807_cov_3.304224, whole genome shotgun sequence.
ACCESSION   DAOPPA010000568 DAOPPA010000000
VERSION     DAOPPA010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70258_length_1807_cov_3.304224"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1807)
                     /gene="pnp"
                     /locus_tag="PKH54_13225"
     CDS             complement(<1..>1807)
                     /gene="pnp"
                     /locus_tag="PKH54_13225"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOD00902.1"
                     /translation="GNDYRDGGFIPLTVDYLERTYAAGKIPGGYFKRESKPTEGEVLN
                     SRIMDRPIRPLFPKTFKYETQIVALVISSDQENDPAVPALIGASAALTFSNVPFQGPI
                     AGIRVGMIGDRLIANPTNEERALCDVDLMTAVGPNGLVMVEGEARFAPEAKIVKALKF
                     AEEASRVLLDLQKDMAAKLNVTKRVPPEKTENAALVARVRELAWDPMCRALTIREKLP
                     RRDAINNLAKATVEAMKEEFPDSTGEILAAIEHLEGERLRGQILDAGLRVDGRDLTTV
                     RPITCEIGALPRTHGSAIFTRGETQALVTVTLGTEEDEQRMDLLTGDHFRRFMLHYNF
                     PAFSVGEVKRLGSPGRREVGHGNLAWRGVSKVLPLDNPEFKYAIRAVSEILESNGSSS
                     MATVCGSSLALHDAGVPVTSHVGGIAMGLIKEGDRVAVLTDILGDEDHLGDMDFKVVG
                     TREGVTAVQMDIKCDGLTEQIMTTALEQARQARLHIIGKLEEAIPAPRTEYSPWAPRI
                     YTMQISEDRIRDLIGPGGRNIRGVQAESGATINVDDSGMVTIAAVDKASADKAIAMIH
                     ELTDDAEVGRTYLGEVVKIMDFGAFVRILPGVEGLC"
BASE COUNT          304 a          552 c          576 g          375 t
ORIGIN      
        1 ggcacaggcc ctcgacgccc ggcaggatcc ggacgaatgc gccgaagtcc atgatcttga
       61 cgacttcgcc aagataggtg cgcccgacct cggcgtcgtc ggtcagttcg tggatcatgg
      121 ctatcgcctt gtcggcggaa gccttgtcaa cggccgcaat ggtgaccatg cccgagtcat
      181 cgacgttgat ggtcgcgccc gattcggcct ggactccgcg gatgttccgg ccgccggggc
      241 cgataaggtc gcggattcga tcctcgctga tctgcatcgt gtaaatgcgc ggggcccacg
      301 gggagtattc ggtcctgggg gccgggatgg cttcctcgag cttgccgatg atgtggagcc
      361 tggcctggcg ggcctgttcc agcgccgtcg tcatgatctg ctcggtaagt ccgtcgcact
      421 tgatgtccat ctggacggcc gtcacgcctt cgcgggtacc gacgaccttg aagtccatgt
      481 cgccaaggtg gtcttcatcg ccaaggatgt cggtaagcac cgcgacgcgg tcgccttcct
      541 tgatgagccc catggcgata ccgccgacat ggctcgtgac ggggacgccg gcgtcgtgca
      601 gggccaggga ggaaccgcag acggtggcca tcgaggaaga gccgttggat tcgaggattt
      661 cggagactgc cctgatggcg tacttgaatt ccgggttgtc gagcggcagg accttcgaca
      721 ctccgcgcca ggcaaggttt ccatgcccga cttcacgacg tccggggctg cccaggcgct
      781 tgacctcgcc gacgctgaac gccgggaagt tgtaatgaag catgaaccgg cggaagtgat
      841 cgccggtcag cagatccatg cgctgctcgt cttcttccgt gcccagggtc acggttacca
      901 gcgcctgggt ctcgccgcgg gtgaaaatcg cgcttccatg cgtgcgggga agtgccccga
      961 tctcgcacgt aatcgggcga accgttgtaa ggtcgcgtcc atcgacgcga aggccggcgt
     1021 caagaatctg gccacgcagc cgttcacctt caaggtgttc gattgcggcc aggatttcgc
     1081 cggtcgagtc cgggaattct tccttcatgg cctcgaccgt cgctttcgcc aggttgttga
     1141 ttgcatcccg gcgtggcagc ttttcgcgga tcgtcagcgc gcggcacatg ggatcccagg
     1201 ccagttcacg gacacgggcg acaagcgcgg cgttttccgt cttttccggc ggaaccctct
     1261 tagtcacgtt caactttgcc gccatgtcct tctgaagatc aagcagaacc ctggaggctt
     1321 cctcggcaaa cttcaacgcc ttgacaatct tggcctccgg agcgaagcgg gcctcgccct
     1381 cgaccatcac caggccgttc gggccgactg ccgtcatcag gtcaacgtcg cacagcgcac
     1441 gttcttcgtt ggtcgggttg gcgatcaacc tgtcgccgat catgccgacc ctgattcccg
     1501 cgatgggacc ctggaaaggc acgttcgaga aggtcagggc ggcgctggcg ccgatcagcg
     1561 cgggaaccgc cggatcgttt tcctggtcgc tggaaatgac cagggcgacg atctgcgtct
     1621 cgtacttgaa agtcttgggg aagaggggcc tgatgggccg atccatgatc ctggaattca
     1681 ggacttcgcc ctcggtcggc ttgctttcac gcttgaagta gcccccgggg attttgcctg
     1741 cggcgtaagt tctttcaagg taatccacgg tgagtggaat gaagcccccg tcgcggtagt
     1801 cgttacc
//
LOCUS       DAOPPA010000569         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_70327_length_1806_cov_2.241005, whole genome shotgun sequence.
ACCESSION   DAOPPA010000569 DAOPPA010000000
VERSION     DAOPPA010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70327_length_1806_cov_2.241005"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PKH54_13230"
     CDS             complement(<1..719)
                     /locus_tag="PKH54_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD00903.1"
                     /translation="MYTFVYSAPDGSGFEQGAWFDGYEIFYGVTEMIPPQYLSRGRAV
                     ADLPANDIIMPILSPDNRYLVGITAFGVLKSLDLQDSGLWRPLAANAGRLQVSPDSTF
                     VAFGDRDNRNMLYSVPIDGSGDARLLTTVMTDSYSQNFMVTPDSSRVVFNEKGVTPTN
                     SMVTIHSADPVSGSTWTLCQSDSWSSLHMTPDGRTIVCHYSKGSTVTIASIADGTVLY
                     TQTDARGFSALPGGTFVVTVDT"
     gene            complement(768..1682)
                     /locus_tag="PKH54_13235"
     CDS             complement(768..1682)
                     /locus_tag="PKH54_13235"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD00904.1"
                     /translation="MNSMHRTMLTMAAAIVFGGGLEGCGTDENCNCPDGNAACDIVEN
                     QDTNLQDLAPDNGEDSYPEDMETPDEAVVPDSDAGLTDVSLGGTATIRGRVTFTNMTD
                     HGGIIIRLSDDMNPAWTGASTVTTTGGDYEFTTVPEGKYRIKATYEGFTVEKSRTALD
                     VEVADGAVVVIPDMTLTATGIIRGRAVFGESATGPGGNDAGIMVYLPGREHISVTRAD
                     GSYEFAGLLPGKYRVCAALTNYAGSCLDWVAVTGLKVSDPSVITIWPEFSLEVPWNRI
                     SGRATMVGQEDNAGISVTAIESGMVRGT"
BASE COUNT          336 a          545 c          512 g          413 t
ORIGIN      
        1 gtatccaccg tcaccacgaa ggtgccgccc ggtaacgcgc tgaagcccct tgcgtcggtc
       61 tgtgtgtaca ggacggttcc gtccgcgatg cttgcgatcg tcacggtcga acccttgctg
      121 tagtgacaga cgatggtcct tccgtccgga gtcatgtgga ggctggacca cgagtcggac
      181 tggcacagtg tccaggtcga ccctgaaacc gggtcggccg aatggatcgt gaccatcgaa
      241 ttcgtgggcg tcacgccttt ttcgttaaag acgacccggg aagaatccgg agtgaccatg
      301 aagttctggc tgtagctgtc ggtcatgacc gtcgtaagca accgtgcgtc gccgctgccg
      361 tcgatgggca cggaataaag catgttgcga ttgtcccggt ctccgaacgc cacgaacgtg
      421 gaatcggggg aaacctgcag tcgccccgcg ttggctgcca gcggccgcca cagcccggaa
      481 tcttgaagat caagtgattt caggacgccg aaggcggtga tgccgaccag ataacgattg
      541 tcgggtgaca atatcggcat gattatgtcg tttgcgggaa ggtctgccac cgcacgtccg
      601 cggctgaggt actgcggcgg gatcatttca gtcacgccat aaaagatttc gtatccatcg
      661 aaccaggcgc cctgttcgaa gccgcttccg tccggcgcgc tatacacaaa tgtgtacatg
      721 ccggcaataa gatcctccat attccagttt ccttcggtgt cggtcgtcta ggtgccgcga
      781 accattcccg actcgatcgc ggtaaccgat atgccggcat tgtcttcctg gccgaccatc
      841 gtcgcccgac cgctgatcct gttccatgga acctccagac tgaactcggg ccagatcgtg
      901 attacggaag ggtcagatac cttcaggccg gtgactgcaa cccaatccag gcagcttccc
      961 gcgtagttcg tcaaggccgc gcagaccctg tattttcctg gcagcaggcc ggcaaattcg
     1021 taactgccgt cggccctggt aacagaaatg tgttccctgc cgggcagata aaccatgatt
     1081 ccggcatcgt ttccgcccgg tccggtcgcg gattccccga aaacggcccg gcccctgatt
     1141 atcccggttg cagttaatgt catgtccggg ataacgacca cagctccgtc cgccacctcg
     1201 acgtccagcg ctgtcctgga tttttcgaca gtgaagcctt cataggtcgc tttgattcgg
     1261 tactttccct caggcacggt agtgaactcg tagtctccgc ctgtcgtggt gactgtcgac
     1321 gcccccgtcc aggccggatt catgtcgtct gaaagcctga ttatgattcc accatggtcg
     1381 gtcatgttgg tgaaggtcac ccgccccctg atggtggcgg ttccgcccag cgaaacgtcc
     1441 gtcagtccgg cgtccgagtc gggcacgacc gcttcatcgg gcgtttccat gtcttccggg
     1501 tacgaatctt cgccgttgtc gggcgcaaga tcctgcaggt tggtgtcctg gttctcgacg
     1561 atgtcgcagg cggcgttccc gtccggacag ttgcagtttt cgtcagtgcc gcagccttcg
     1621 agcccccccc cgaagacgat tgcggcggcc atggtgagca ttgtccgatg catgctgttc
     1681 atagatcccc ccctttttgt aaaagccact atacgtcagt ctgctcgtct atttgactgt
     1741 agtcaatggc ttgcccaaat aagattctaa ggttcattgg gcgatgacct agcggtgcct
     1801 gtcgcc
//
LOCUS       DAOPPA010000570         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_70414_length_1804_cov_4.703831, whole genome shotgun sequence.
ACCESSION   DAOPPA010000570 DAOPPA010000000
VERSION     DAOPPA010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70414_length_1804_cov_4.703831"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKH54_13240"
     CDS             <1..830
                     /locus_tag="PKH54_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOD00905.1"
                     /translation="KYSITWSLDDYDTWGGLENRHKFIVGLPLYGHDWPAIGETPPSE
                     SAGKADSVFFTACRQGESEHGRNWDQDSASPWYGYYDGQQYRQVWCDDSWSLALKMAP
                     ARDRGLGGIGFWAVGYEGSLDGPWQAVVDVFGLGGPDDPPDQVEIGADEAVPPEVPEF
                     VDSQDAAVVDFDPLPDSGGSDPAPSEDDGGKVDPGFRPDLDDTKDVAAGGFELQVELN
                     AGDTIQDHDRQPTTSCSASGRSGPIPVGLIIAWVVLIFGLARQILSTRRHRQGRAAA"
     gene            886..1602
                     /locus_tag="PKH54_13245"
     CDS             886..1602
                     /locus_tag="PKH54_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD00906.1"
                     /translation="MDFIDRVVMVLVPMLLSLTVHEYAHARSAFALGDDTAARMGRMS
                     LNPVVHIDVFGTIILPLISVISGSSFFFGWARPVPVDPTGFTRRISMKRGMLLTAAAG
                     PLSNLAFALVLAVIFKIMSLAGAVDFPFIATLPGQADAWVSMTGNEVPAIYRLIGTTF
                     VINVVLAIFNFIPVPPLDGSRVIVGLLPDSIGARFAWLERNPIFIIVMFSLLIFGGAR
                     FLSGPVNAILFGLLKITGNV"
     gene            complement(1586..>1804)
                     /locus_tag="PKH54_13250"
     CDS             complement(1586..>1804)
                     /locus_tag="PKH54_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00907.1"
                     /translation="VRPGGRVVFATCSFSPTEGRDVLAAVLGARPGYELVETGLPTAL
                     ESPDHTVTILPWAFEMDGFFIAAIRRCP"
BASE COUNT          298 a          544 c          555 g          407 t
ORIGIN      
        1 gcaagtactc aatcacctgg tcgcttgacg attacgacac gtggggcggg cttgaaaacc
       61 gtcacaagtt catcgtgggc ctgccgctgt acggccatga ctggccggcg atcggtgaaa
      121 cgccaccttc cgaatcggcc ggcaaggcag actccgtctt tttcaccgcg tgccgacagg
      181 gggagtcgga gcatggtcgg aactgggatc aggattcggc ttcgccatgg tacggctatt
      241 acgacggcca gcagtaccga caagtctggt gcgacgattc ctggtcgctc gcgctgaaga
      301 tggcgccggc gcgggatcgc ggtcttggcg ggattggatt ctgggctgtt ggatatgaag
      361 gttcgcttga tggtccctgg caggctgtcg tcgatgtctt cggtcttggc ggccccgacg
      421 atccgccgga tcaggtcgag atcggggcgg acgaggcggt tccgcccgaa gtgccggagt
      481 tcgtcgacag ccaggacgcc gccgtcgtcg atttcgatcc gcttccggat tccggcggtt
      541 cggatcccgc cccttccgag gatgacggcg gcaaggtcga tcccggtttc cggcccgatc
      601 tggacgacac gaaggacgtg gccgcaggag gtttcgagct tcaggttgaa ctgaatgccg
      661 gcgacacgat acaagaccat gatcgccagc caacaacgtc ctgcagcgcc agcgggcgct
      721 cgggcccaat ccccgtcggc ctgattattg cctgggtggt attgattttc ggccttgcaa
      781 gacagatatt gtccacgcgt cggcaccgcc agggacgtgc cgccgcctga cctgccgcct
      841 tgacgcgtcc gggtccggtt caatacatga ttcccggggg ttgacttgga tttcatcgat
      901 cgtgtcgtga tggttctcgt tccaatgctg ctttccctga ccgtgcatga gtatgcccac
      961 gccaggtcgg ccttcgcgct tggcgacgat accgccgcca ggatgggcag gatgagcctt
     1021 aatccggtgg ttcatatcga tgttttcggc accataatcc ttcccctgat ttcggtcatt
     1081 tccggaagtt cgttcttttt cggctgggcc cgcccggttc cggtcgatcc gaccggtttc
     1141 acgcgccgga tatcgatgaa gcgcggcatg ctgctgacgg ccgcggccgg ccctctgtcg
     1201 aacctggcct tcgcgctggt acttgccgtg atcttcaaga tcatgtcgct tgccggggcc
     1261 gtggattttc cgttcatcgc gacgcttccc ggccaggccg acgcctgggt ttcaatgacc
     1321 ggcaacgaag tcccggccat ttatcgtctg attggaacca ctttcgtcat caacgtcgtt
     1381 cttgccatat tcaatttcat cccggtgcct ccactggatg gaagccgcgt gatcgtcggg
     1441 ctgctgccgg acagcatcgg cgcaaggttc gcctggctcg agcgcaaccc gatcttcatc
     1501 atcgtcatgt tttcgcttct gatctttggc ggggcaaggt tcctttccgg tcccgtgaac
     1561 gcgattcttt ttggattgct gaagattacg ggcaacgtct gatagccgcg atgaagaatc
     1621 cgtccatttc gaaagcccag ggaagaatcg tcaccgtgtg atccggtgat tcgagtgccg
     1681 tcggaagtcc ggtttcgacc agttcgtatc ccggcctggc gcccagcacc gccgcgagaa
     1741 cgtcgcgacc ttcggtcggg ctgaacgaac aggtggcgaa cacgaccctg ccgccgggcc
     1801 tgac
//
LOCUS       DAOPPA010000571         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_70535_length_1802_cov_4.554665, whole genome shotgun sequence.
ACCESSION   DAOPPA010000571 DAOPPA010000000
VERSION     DAOPPA010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70535_length_1802_cov_4.554665"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PKH54_13255"
     CDS             complement(<1..1415)
                     /locus_tag="PKH54_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00908.1"
                     /translation="MMSRSRRRKEQGHRIRGIFLTFSMTAITMFMVTVGCGGDAGPTD
                     TLNSDAIDTLETDTGDQPGGVVLHADLSGLDADETAWLHVGARVYPMKAHTDETRDLY
                     LNQNGLQGTIDDEDVTHYAEGVDLPDDRVALMWVTHSPEDDRDGEHGLGLVGLHIPQV
                     AMEKARTLRAYQGWPRYRQYLDNPMPKRNAQFVPKKLVKRDANDDPDDMLTPLDTAKA
                     LLFHHPAVTSLDPDVAALVMTHIENAVGLSELTDAISAQGRAYEHDDDYEDGWAVLVP
                     ILDEEGNRRMKSDGTPYYHYVLSDRTAEEAETAVKSLLRAAHNDLELRDKTWHVQDGT
                     GAIDASPEPVATHTLRAAGAYDWTLDYTNSRSGLEARVEALSNNPDNGLRRVKIGLKN
                     HWLRHLTFYVGFVKADGTRMTIPASMATSHDDKDHDIMYVGKVPPVVEIMSIPLPADW
                     VDFTIDVPNEAQSIEIMGGGLG"
     gene            complement(1589..>1802)
                     /locus_tag="PKH54_13260"
     CDS             complement(1589..>1802)
                     /locus_tag="PKH54_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00909.1"
                     /translation="GVDKEFHFLTEVGEDVAPTYGGVGNIDHVVSNILEGSCWAAGVT
                     EGHPPVIVDTVFDHVPIVCDVAMPSL"
BASE COUNT          316 a          550 c          534 g          402 t
ORIGIN      
        1 ccaagcccgc cgcccattat ttcgatggat tgcgcctcgt tgggcacgtc gatggtgaaa
       61 tcgacccagt cggcaggaag cgggatgctc atgatttcga cgaccggcgg gaccttgccg
      121 acgtacatga tgtcgtgatc cttgtcgtca tgggaagtcg ccatcgaggc tgggatggtc
      181 attcgcgtgc cgtccgcctt aacgaatccg acatagaacg taaggtgtcg cagccagtgg
      241 ttcttcaggc cgatcttcac gcgccgcagg ccgttgtccg ggttgttgga aagggcctcg
      301 acgcgggcct cgaggccgga acgtgagttg gtgtaatcga gtgtccagtc ataggcgccg
      361 gcggcacgca gggtgtgtgt tgccaccggt tccggggatg catcgatcgc gccggttccg
      421 tcctggacgt gccaggtctt gtcccgcagt tcgagatcat tgtgggcggc ccgaagcagg
      481 gacttgacgg cagtctcggc ctcctccgcc gtcctgtccg acagaacgta gtgataataa
      541 ggcgttccat ccgatttcat tcggcggttt ccttcctcgt ccagaatcgg caccagaacc
      601 gcccagccgt cctcgtaatc gtcgtcgtgt tcgtatgcgc gcccctgcgc cgatatcgcg
      661 tccgtcagtt cggaaagccc cacggcgttt tcgatgtgcg tcatcaccag ggccgccacg
      721 tccggatcaa ggctcgtgac tgccggatgg tggaagagaa gcgcctttgc ggtgtccagc
      781 ggtgtgagca tgtcgtccgg atcgtcgttg gcgtcccgtt taacaagctt cttcggcacg
      841 aactgcgcat tgcgcttcgg catggggtta tcaaggtact gccggtatcg cggccagccc
      901 tgataggcac gcagggttct ggccttctcc atcgcgacct gcggtatatg cagcccgacc
      961 aggccaagac cgtgctcgcc atcgcgatca tcttccgggc tgtgcgtcac ccacatcagg
     1021 gcgacacggt catccggcag atctacgccc tcggcatagt gggtcacgtc ttcatcgtcg
     1081 atcgtgccct gcaatccgtt ctggttcagg tacaggtcgc gggtctcatc ggtgtgggcc
     1141 ttcatcggat agaccctggc gccgacatgc agccaggccg tctcgtccgc atccaggccg
     1201 gaaagatccg cgtggagcac cacgcccccc ggctgatcgc ccgtgtctgt ttccaaagta
     1261 tcgattgcgt ccgaattcag cgtgtcggtg ggccccgcat cgcccccgca gccgacggtg
     1321 accatgaaca tggtaatcgc ggtcattgag aaggtcagga agatcccccg aatcctgtgg
     1381 ccttgttcct tcctccgccg acttcttgac atcatggttt tcccctttgc tgcaaggatg
     1441 caggaatgat ttgcctgcat actatacctt cgcttttcaa gcatggcgac attgatatgc
     1501 cgcgcgtcag ccattttcgt gccggcgagc ctgttcaacc ttgccggaat aatcttggtg
     1561 tcgcggactt atgctgatcg gggtgccgtc acaatgatgg catggcgaca tcgcatacga
     1621 tggggacgtg atcgaaaacg gtatccacga tgacgggtgg atgtccctcg gtaacgccgg
     1681 ccgcccagca ggagccttca aggatgttgc tgacgacatg atcgatgttt ccgacaccac
     1741 cgtacgtcgg cgcaacatct tccccaacct ccgtcaggaa atggaactcc ttgtccacgc
     1801 cg
//
LOCUS       DAOPPA010000572         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_71346_length_1788_cov_3.815349, whole genome shotgun sequence.
ACCESSION   DAOPPA010000572 DAOPPA010000000
VERSION     DAOPPA010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71346_length_1788_cov_3.815349"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKH54_13265"
     CDS             complement(<1..1113)
                     /locus_tag="PKH54_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOD00910.1"
                     /translation="MRRIIAVAVLTSLVALCACTPDEVSPPTNLSRLRVENKNFRDES
                     GRYLMINGVNVSGTSKVPVKISPVSYVGRPFPIDQVDDWFAKLRRDGFNAMRLLVIWE
                     AVCHQAPGVYDNEYLDYLEQVISKANDHGIYVLINFHENLFSRHLYSVFNDHPTIGEP
                     GSWQAMIGSLFPATDPETGELYYDSKVVGDGAPRWAVEACLPEKDLDAPSYGMLRILG
                     DVTMDSGNLGKLISAFTTLLAGGDGGMTPEIIELIDMLSSSLAAMTDAGLLPFDMTTT
                     NDMMPFTLWAMNSFLSVDVEKCYAAFFAGNEVFPDRKIDGVPIMDYLQRGHIDAWLQV
                     VERAKKYPNILGYDLMNEPTGIFIVLSVVSAYVSAGF"
     gene            1333..>1788
                     /locus_tag="PKH54_13270"
     CDS             1333..>1788
                     /locus_tag="PKH54_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00911.1"
                     /translation="MNSTRQSGFCLMVLLLLLLSSCSATPDQGTDPGPESVEFDAIPN
                     TFDAVEALDVFDSAEVRADSFQDLTDANDVATGGFGVTDGAEDTDALPPDTASDVLVC
                     GDVPKEGCPCDEAKDEPCCIMIAQGLSCEPSRWVDGVFVVEWGVFWDCGC"
BASE COUNT          385 a          512 c          528 g          363 t
ORIGIN      
        1 gaaaccggcc gaaacgtagg cggaaacgac cgacaggacg atgaagatgc cggtgggctc
       61 gttcatcagg tcatatccca ggatattcgg gtacttcttc gcgcgctcga caacctgcag
      121 ccacgcgtcg atgtggcccc gctgcaggta gtccatgatc gggacgccgt cgatcttgcg
      181 gtccgggaag acctcgttgc cggcgaagaa ggcggcgtag cacttttcga catcgaccga
      241 cagaaacgaa ttcattgccc acagcgtgaa cggcatcatg tcattcgtgg tggtcatgtc
      301 gaacggcagc aggccggcat ccgtcatcgc ggccaggctt gacgacagca tgtcaatcaa
      361 ttcaatgatt tccggggtca ttccgccatc gccgcccgcc agaagcgtcg tgaacgccga
      421 aatcagcttg cccaggttgc cgctgtccat cgtgacgtcg cccagaatgc gcagcatgcc
      481 gtacgacggc gcatccagat ccttttcggg caggcacgcc tcgaccgccc agcgcggcgc
      541 gccgtcgcca acgaccttgg aatcgtaata aagttcgccg gtttcaggat ccgtggccgg
      601 gaacaggctg ccgatcatag cctgccagct tcccggctcg ccaatcgtgg gatgatcgtt
      661 gaagaccgaa tatagatggc ggctgaaaag gttttcgtgg aaattgatca ggacatagat
      721 gccgtgatcg ttcgccttgg aaatcacctg ttccaggtag tccaggtatt cgttgtcata
      781 aacgccaggc gcctgatggc agaccgcttc ccagatgacc agcaggcgca tggcgttgaa
      841 gccgtcgcgc cgcagcttgg cgaaccagtc atcgacctgg tcgatcggga acggacggcc
      901 cacgtacgaa accggcgaga tcttgaccgg gaccttcgac gtgccgctga cgttgacacc
      961 gttgatcatc aggtaacgcc ccgattcatc gcgaaaattc ttattttcaa cgcgcaggcg
     1021 ggacagattc gtcggcgggc tgacttcgtc gggcgtgcac gcgcacagcg cgacgaggct
     1081 ggtcagaacc gccacggcaa tgattcgacg cattggaacc tcctgggaaa atgacggaca
     1141 accggaaccc tggccgggac cgcgaaacga tgtggaaatc ctagtacagc ggcacatgac
     1201 gcacaacagc agaagttaca cggcgcggca cgcctataca tcattttcgc catacttatt
     1261 gatttgacga tatacaatcc gctagcattg ctgcaaccct cttgcccgga aaaacatctg
     1321 gagacgtaca ggatgaattc aaccaggcaa tcgggctttt gtttgatggt gctactgcta
     1381 ctgcttctgt cttcatgttc ggcaacaccg gaccagggga cagaccccgg tcccgagtcc
     1441 gtagaattcg acgcgatccc gaacactttc gatgcagtcg aggcgctgga tgtttttgat
     1501 tctgcagaag ttcgcgccga ttcgtttcaa gacctaaccg acgcgaatga cgtcgccacg
     1561 ggcggctttg gggtcactga cggcgctgag gacacggacg ccctgccccc ggacaccgca
     1621 tcggatgttc ttgtctgcgg ggacgtaccc aaggaaggat gcccgtgtga cgaagcgaaa
     1681 gacgagccgt gctgcatcat gatcgcacaa ggtttgtcat gtgagccttc ccgctgggtg
     1741 gacggggtgt ttgttgttga gtggggcgtt ttctgggact gtggctgt
//
LOCUS       DAOPPA010000573         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_71453_length_1786_cov_3.710572, whole genome shotgun sequence.
ACCESSION   DAOPPA010000573 DAOPPA010000000
VERSION     DAOPPA010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71453_length_1786_cov_3.710572"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH54_13275"
     CDS             <1..224
                     /locus_tag="PKH54_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00912.1"
                     /translation="GINAQELPLFHQLDIRVDKEWVFKAWRLGLYLEVQNVYFHKNPE
                     GVIYNYDYSKMAYLTGLPILPVLGIKGSF"
     gene            complement(336..>1786)
                     /locus_tag="PKH54_13280"
     CDS             complement(336..>1786)
                     /locus_tag="PKH54_13280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD00913.1"
                     /translation="EFSHILNGDMRLNIRLIGFISGIMVLATIGYWALHMRPSSRGKN
                     NGYAAILLAAIALLIIGYGGAFVARLIQSAVSRQREFLADSSAVQFTRNPSGIANALK
                     KIGGYEIHSTIHSPASREVRHMFFGPSDVVSMLGSVLATHPPIDERIKRLDPSFDGDY
                     SKRSVLPDLQAPEAAAGQAGVAGMVGAAAFGSAGASARAVSSGSPTLPTSAESVAGMG
                     VLTQEHIDAGAALLAAIPDRLRQGVSEPMSACATIAALLLDSDPDVRSRQLESVAREF
                     SPALARETGLVAKDVASIDPAFRLPLVDLALPALRRLSRPQYAAFSRTLIELVRADEN
                     VTLFEFCLQKVVEFRLFQAFDAASRGYRAVSPREFIGDAVVLLSAVARAGSSVEDDAR
                     RSFEAAVSTLFMGRVPAGVGPLQKPGIDQLDQAFQRFSMAPMKIRRQVFLAVSDCVLH
                     DGHVTVQESELIRAVAYCMGLPLPPFVVGQAA"
BASE COUNT          376 a          538 c          569 g          303 t
ORIGIN      
        1 cggggatcaa cgcacaggag ctgcccctgt tccaccagct tgacatccgc gtcgataagg
       61 aatgggtgtt caaggcgtgg cgtcttggac tgtacctgga agtccagaac gtctatttcc
      121 acaagaaccc cgaaggcgtg atctacaact acgattactc gaagatggcc tatctgaccg
      181 gtcttccgat acttcccgtg ctgggcatca agggttcgtt ctgaaggccg ggctgccggg
      241 gtatcggtgg tggccggact ggcctgactg gccgggtggc cggggtgtcc agcttgttct
      301 gtatgtccgg ggtatcctga cgacggcccg cgctttcagg cggcctgtcc gaccacgaaa
      361 ggcggaagcg gcaatcccat gcaataggca acggcgcgaa tcagttcgga ttcctgaacg
      421 gtcacatggc cgtcgtgcag cacgcagtcg gaaacggcaa gaaatacctg ccgtcttatc
      481 ttcatcgggg ccatcgagaa cctctggaat gcctggtcaa gctggtcgat gccgggcttc
      541 tgcaacggcc cgaccccggc ggggacacgg cccatgaaga gcgttgaaac ggcggcttca
      601 aacgaccggc gggcgtcgtc ttcgacactg gaaccggccc tggcaacagc cgaaagaaga
      661 acgaccgcat caccgatgaa ttccctgggg gaaacggcgc ggtaaccgcg gcttgcggcg
      721 tcgaacgcct ggaacaacct gaattcgacg accttctgaa ggcagaactc gaacagggtg
      781 acgttttcgt ccgcgcgcac aagttcgata agcgtccgtg aaaaggccgc gtactgcgga
      841 cgcgacaggc ggcgaagcgc cggcagcgca agatccacca gaggaaggcg gaaagcggga
      901 tcaatcgagg ccacgtcctt cgcgacaagc cccgtttccc gggcaagggc cggcgagaat
      961 tcacgggcga cggactcaag ctgacgcgaa cggacatcgg gatccgaatc gagaagaagc
     1021 gccgcgatgg tcgcgcaagc gctcatcggc tctgaaacgc cctgtcgcag acgatccggg
     1081 atggcggcca gaagtgccgc gccggcgtcg atatgttcct gtgtcaggac tcccattccg
     1141 gcaaccgatt ccgccgacgt cggcaaggtt ggcgaaccgg aagaaacggc gcgggcggac
     1201 gcacctgccg aaccgaaggc ggcggctcca accattcccg ccacgccggc ctgtccggcc
     1261 gcggcttccg gcgcctgcaa gtccggcagc acgctccttt tcgaatagtc tccatcaaag
     1321 gacggatcaa gacgcttaat cctttcatcg attgggggat gtgtcgccag gaccgagccg
     1381 agcatggaga ccacgtccga cgggccgaag aacatgtgac gcacttccct ggacgcaggc
     1441 gaatggatgg tgctgtggat ttcgtacccg ccgatcttct tcagcgcatt ggcgatgccg
     1501 gacggattcc tggtgaactg gaccgcgctg gaatccgcaa ggaactcgcg ctggcgcgac
     1561 acggcgctct ggatcagcct ggcgacgaag gcgccgccgt aaccgattat cagcagggca
     1621 atcgcagcca gcaggatcgc ggcgtatccg ttgttcttgc cacgcgacga aggacgcatg
     1681 tgaagcgccc agtatccgat cgtcgcaagc accatgatcc cggagatgaa accgatcagg
     1741 cggatgttca ggcgcatgtc gccgttcagg atgtggctga actcgt
//
LOCUS       DAOPPA010000574         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_71914_length_1778_cov_3.446315, whole genome shotgun sequence.
ACCESSION   DAOPPA010000574 DAOPPA010000000
VERSION     DAOPPA010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71914_length_1778_cov_3.446315"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKH54_13285"
     CDS             <1..270
                     /locus_tag="PKH54_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD00914.1"
                     /translation="VGDACDNCNGQPNAGQVDSDFDGVGNVCDNCPGTPNADQSDVDN
                     DGLGDKCDPDADADGIANIDDNCWLVPNPDQHDEDLDGKGDACDD"
     gene            328..>1778
                     /locus_tag="PKH54_13290"
     CDS             328..>1778
                     /locus_tag="PKH54_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00915.1"
                     /translation="MKRLTMVLCLAVSAAVVSGCIPAQVSLKDVDVTKLELPVLKKSM
                     DRVHSEIAARADGQLYVGAAEVDITTRYGHEGSIYIGGFDLGRKAKGVKDPVYAHAIY
                     IDDGKMPYVLVTIDTIGYMNNEVDAVRELASDLHRERINVASIHNHVGPDTIGYWGPA
                     DGGLIPVRTGVVPEYMITLRHLIAQAIDQAAESARPAKLKFSTANIDPQLSLNIHANI
                     RMQKDDVARIMTAVGVDGKPIALLANYGNHVEAMWNDEYISADWAGVFYDRVRKELGA
                     VPLLVEGALGGLVTINPGDDKMAMEKEIMAVFLKHMTRDERIELTNRIGNSFADQVIA
                     AANTSSRIAGPTGLSLRYASTEFYIKVSNFVFEYFYNRGFFDRPMKYRKGVPFMSSDV
                     SIMRIMEGDAALADFTTWPGEPAPTVVAELDAGSPAEFRFTVSLGNDEMGYMVLENDW
                     NNAEYGYERSMSLGKHATTEVMQHINALRDLLK"
BASE COUNT          410 a          441 c          549 g          378 t
ORIGIN      
        1 gtcggcgacg cgtgcgacaa ctgcaacggt cagccgaatg caggccaggt cgattccgat
       61 ttcgacggcg ttggtaatgt ttgcgacaac tgtcccggga ctccgaatgc tgatcagtcg
      121 gatgtcgata acgacgggct gggcgacaag tgcgatcccg atgcggacgc cgacggcatc
      181 gcgaacattg atgacaattg ctggctggtt ccgaatcccg atcagcatga cgaggatctg
      241 gacggaaagg gcgacgcctg cgacgattga tgtttcctgg cgaattgggc ttggattaca
      301 aggttttatg aatttcaggg gaaagcgatg aagagactga caatggttct ttgcctggcg
      361 gtgtcggccg cggtggtttc cggctgtatt ccggcccagg tttcgttgaa ggatgtcgat
      421 gtcaccaagt tggaactgcc ggttctgaag aagtcgatgg atcgtgttca ttcagagatc
      481 gcggcccggg ccgacggtca gctttatgtc ggcgccgcgg aagtcgatat cacgaccagg
      541 tatggacacg aaggcagcat ctacatcggc gggttcgatc ttggcaggaa ggcgaagggt
      601 gtaaaggatc cggtttatgc gcacgccatt tacatcgatg acggaaaaat gccttatgtc
      661 ctggtgacta tcgacactat cgggtacatg aacaatgaag tcgatgcggt tcgggaactg
      721 gcgagcgatc ttcatcgtga aaggatcaac gtggcgtcga tccataacca cgtgggcccg
      781 gacacgattg gctactgggg cccggcggac ggcggattga ttccggtcag gacaggtgtg
      841 gttccggaat acatgatcac gttgcgtcac ctgattgccc aggcgattga ccaggcggcg
      901 gaatcggccc gcccggccaa gctgaagttc agcaccgcca atatcgatcc gcagctctct
      961 ctgaatatcc acgcgaacat caggatgcag aaggacgatg ttgcaagaat tatgacggcc
     1021 gtcggtgttg acggaaagcc gattgccctg cttgcaaact atggaaacca cgtcgaggcc
     1081 atgtggaacg atgaatacat cagcgccgac tgggccgggg tgttttatga ccgtgtcagg
     1141 aaggaacttg gcgcggttcc gctgctcgtc gaaggcgcgc tggggggact ggtcacgatt
     1201 aatccaggcg acgacaagat ggccatggaa aaggaaatca tggcggtctt cctcaaacat
     1261 atgacgcgtg acgaaagaat tgagcttacc aacaggatcg gcaacagttt cgcggatcag
     1321 gtcattgccg cagccaacac gtcatcaagg attgccgggc cgaccggtct gtcgcttcgc
     1381 tacgccagca ccgagttcta catcaaggtc agcaatttcg tatttgagta cttctacaat
     1441 cgtggattct tcgatcgtcc gatgaaatat cgaaagggcg ttccgttcat gagttccgat
     1501 gtcagcataa tgcggatcat ggaaggcgat gccgcgcttg ccgatttcac cacgtggcca
     1561 ggcgagccgg ctccgacggt ggttgccgaa ctggatgccg ggtcgcccgc ggaattccgc
     1621 ttcaccgttt cgcttggaaa cgacgagatg ggatacatgg tcctggaaaa tgactggaac
     1681 aacgccgaat acggctacga gcgaagcatg tcgcttggaa agcatgcgac gacggaagtg
     1741 atgcagcaca tcaacgcgtt gcgcgatctt ttgaagta
//
LOCUS       DAOPPA010000575         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_72424_length_1769_cov_3.624854, whole genome shotgun sequence.
ACCESSION   DAOPPA010000575 DAOPPA010000000
VERSION     DAOPPA010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72424_length_1769_cov_3.624854"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="larB"
                     /locus_tag="PKH54_13295"
     CDS             <1..422
                     /gene="larB"
                     /locus_tag="PKH54_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOD00916.1"
                     /translation="DGFVAVVTAGTSDIPVAEEAAVTLETCGNRVVRIRDVGVAGIHR
                     LGIHIRTLQEASAVIVAAGMEGALPGIVAGLTSAPVIGIPTSVGYGTSFGGITALLAM
                     LNSCAGGLTVVNIDNGYGAAVAAHLINARTGRAGGAK"
     gene            419..1687
                     /gene="larC"
                     /locus_tag="PKH54_13300"
     CDS             419..1687
                     /gene="larC"
                     /locus_tag="PKH54_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOD00917.1"
                     /translation="MSRNIVYLQCTAGIAGDMTLAALIAAGADAGYVRDAAEAVAGRP
                     VGFAVTPCRKSGISGLRLELDLPRDHHHRSMKQIEDMITTAARDNLIAGRVAQRALKI
                     FQIMAVAEASVHGVEPEDVEFHEIGALDSIVDIVGVAAALESLQIDTVTASPLPMTAG
                     MVATMHGSMPVPAPATVRILKGWRLYGQDAPGEFVTPTGAAICVALAGTASAWPRMTL
                     VSDGFGFGNLEWPDGRPNCVRAAVGTTDDCDGEAFQAFQDGPSRTGETVFTIEANIDD
                     STPEQISRLAAALMEAGALDAWVCPVLMKKGRPGWVVSALCGACDVKRLSRVFFLESS
                     TIGVRYRESGRFTLPRSFSTFSSSLGDVTVKTTTLDGEVMGRNPEADEVARLAATAGL
                     STAQARRRIEAEMELDGPLPGNTSENVPRP"
BASE COUNT          331 a          536 c          570 g          332 t
ORIGIN      
        1 ccgatggctt tgttgccgta gttacggccg gaacgtccga cattccggtt gccgaggaag
       61 cggcggtaac gctcgagact tgtggaaacc gcgtcgtcag gatccgggac gtgggagtcg
      121 cggggatcca tcgcctgggc attcatatac ggacgcttca ggaagcttcg gctgtcatcg
      181 tcgccgccgg aatggaaggg gcgctgcccg gaatcgtggc cgggctgacg tcggcacctg
      241 tcatcggcat tccaaccagc gtcggatacg gcacctcctt cggtgggatt acggcattgc
      301 tggcaatgct caattcctgc gccggcggcc tgaccgtggt caatatcgac aacggctacg
      361 gggctgccgt ggccgcgcac ctgattaacg cccggacagg gcgcgcggga ggggcgaaat
      421 gagccggaat atcgtttatc ttcaatgcac ggccgggatt gccggggaca tgacgcttgc
      481 cgcgctgatt gcggccgggg cggatgccgg atacgtcagg gacgctgccg aggcggtggc
      541 cgggcgcccg gttggattcg ctgtcactcc atgtcggaaa tcaggcatat ccggactgcg
      601 gctggaactg gatctgcccc gcgatcacca tcacagatcg atgaagcaga ttgaagacat
      661 gattacgacg gccgcacggg acaacctgat cgccgggagg gtggcgcaaa gggcgcttaa
      721 gatttttcaa atcatggctg tcgccgaggc ttcggtgcac ggcgtcgaac cggaagacgt
      781 ggagtttcac gaaatcggcg cgcttgattc aatcgttgac atcgttgggg tcgcggccgc
      841 cctggagtcc cttcaaatcg ataccgtcac cgcctcccca ctgccgatga ccgccggtat
      901 ggtcgccacg atgcacggca gcatgcccgt cccggcccct gccaccgtca ggatcctgaa
      961 gggctggcgc ctctatggcc aggatgcccc cggggaattc gtaacgccca ccggtgcagc
     1021 catctgcgtc gccctggccg gcacggcatc ggcatggccc cggatgaccc tggtttccga
     1081 cggattcggg ttcggaaacc ttgagtggcc ggacggacgc cccaactgcg tgcgggcggc
     1141 ggtcggaacg acggatgact gcgacggtga agcgttccag gcctttcagg acgggccttc
     1201 ccgcacaggt gaaaccgttt tcacaatcga ggcaaacatc gacgattcca cacccgagca
     1261 gatttcccgc cttgccgcgg cccttatgga agcgggggca ctggacgcct gggtctgccc
     1321 ggttctgatg aagaagggac gccccggctg ggtggtatcg gcactgtgcg gcgcgtgcga
     1381 cgtgaaaagg ctgtcgcggg tcttcttcct tgaatcctcg acgatcggcg tcaggtaccg
     1441 ggaaagcggc aggttcacgc tgccaaggtc gttttcaacc ttctcatcgt cactcgggga
     1501 tgtgacggtc aaaacgacaa cccttgacgg tgaagtgatg gggcgaaacc ccgaagccga
     1561 cgaggttgcc cggctggctg ccacggccgg tctttccacc gcacaggcaa gacgccggat
     1621 cgaggccgaa atggaactgg acgggccgct cccgggaaac acgtccgaaa acgttccacg
     1681 gccttaattt tgctgcatga tccagttgtt caaaaccgtc cttgaacgct ttacacacca
     1741 cccctttcgg agtattattc ccgcacctg
//
LOCUS       DAOPPA010000576         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_74223_length_1738_cov_3.551396, whole genome shotgun sequence.
ACCESSION   DAOPPA010000576 DAOPPA010000000
VERSION     DAOPPA010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74223_length_1738_cov_3.551396"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1738)
                     /locus_tag="PKH54_13305"
     CDS             complement(<1..>1738)
                     /locus_tag="PKH54_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOD00918.1"
                     /translation="WWWRSVNTSARVIATGTAVTDDGGSLSASFLAEADEPEDGTPAP
                     DYIFNLSVDVTDEAGETHSGRKSFRLGFSTERATASTPTRFFEPSEDVEIDMSRTDIN
                     GNPAPGGIKWTLARIVSPERTLLPSQSASDDLVDIMEQYEGGHFSGGYDADRMIREWK
                     DGTGIRDGTVQTDSKGSARLGLGRLDAGLWRVRYSVTDQFGAAYDAWFDFKVVSSEST
                     TPLPMFMAAQKPSSEVGQSVRLAVSSAFPNQYALLQVTSRQGRIDTRHLRLDKGTTII
                     DYPVTPAMRGGLTFSLLAYHSYESLAVSTTVAVPWSDRRLDVKIATFRDKVVPGGRET
                     VSIKVSDPDSKKGIGTAEVLTWMYDKALDDIARGYFPDLSGIWPGVKLPLPRFHNLGR
                     AREIWFDSEGFDNPQVSFEQYEPLSLKYLERRWGGRYARGNLGLAAPMATSSGFGRGG
                     GGLMKSRTVSLEMADGMAQEADEESSVGMFAAVAPASAPAPDEAAAVKAEEAAQPQPR
                     TNFAETAFFQPHLLTGTDGSVTFQYVVPDSITTWKLIARAQTMDLRTGGTEQLIVSAR
                     DMMVKPFLPRFLR"
BASE COUNT          305 a          552 c          525 g          356 t
ORIGIN      
        1 cgaaggaagc gcggcaggaa gggcttgacc atcatgtccc tggcagaaac gatcaactgt
       61 tctgtgccgc cggtccgcag atccatggtc tgcgccctgg cgatcagctt ccaggtcgtt
      121 atcgaatccg gcacaacgta ctggaaggtg accgagccgt cggttcccgt cagcaggtgc
      181 ggctggaaga aggcggtttc ggcgaagttg gttcgaggct ggggctgggc ggcttcctcc
      241 gccttgaccg ctgccgcctc gtccggggcc ggggcggatg ccggggccac ggccgcgaac
      301 attccgacag aggattcctc gtcggcctcc tgcgccatgc catcggccat ctcaagtgac
      361 accgtcctgg acttcatcag gccgccgccc ccgcgtccga agccggacga cgtggccatc
      421 ggggccgcca gtccaaggtt accgcgcgcg taccgtccgc cccagcgccg ttcaaggtat
      481 ttcagggaca gcggctcgta ctgttcgaat gatacctggg ggttgtcgaa cccttccgag
      541 tcgaaccaga tttcccttgc ccggcccagg ttatggaatc ttggcagagg caacttgacg
      601 cccggccaga tccctgaaag atccggaaag taccctcggg cgatgtcgtc gagcgccttg
      661 tcgtacatcc aggtcaggac ctcggcggtt ccgattccct tcttcgaatc gggatcggat
      721 accttgatgg aaacggtttc tcgtcccccc ggcacgacct tgtccctgaa cgtggcgatc
      781 ttcacgtcga gtcgtctgtc gctccacggc acggcgacgg ttgtcgaaac cgccaggctt
      841 tcgtacgaat ggtaggcaag cagcgagaat gtcagtccgc cgcgcatcgc cggcgtcacc
      901 gggtagtcga ttattgtcgt tcccttgtcc aggcgcagat gtcttgtgtc gattcggccc
      961 tgccggcttg ttacctgaag cagcgcatac tggttcggaa atgcgctgga aacggccagc
     1021 cgcaccgact gcccgacctc gctggaaggc ttctgcgccg ccatgaacat cggaagggga
     1081 gttgtcgatt cactcgagac gaccttgaag tcgaaccatg cgtcatatgc cgcgccgaac
     1141 tggtcggtca ccgaatacct gacacgccac agaccggcgt caagccgtcc aaggccgagc
     1201 cgtgccgagc ccttcgaatc ggtctggacc gtgccgtccc tgattcccgt cccatccttc
     1261 cattcacgga tcatcctgtc cgcgtcatat ccgcccgaaa agtgaccgcc ttcgtactgt
     1321 tccataatat cgacgaggtc gtccgaagcg gattgcgacg gaagcaaggt cctttccggc
     1381 gaaacgatgc gtgccagggt ccatttgatc ccgccgggcg ccgggtttcc gttgatgtcg
     1441 gttctggaca tgtcgatttc aacgtcttcg gacggctcga agaatcttgt cggcgtcgag
     1501 gccgtcgcgc gttccgtcga gaagcccagc ctgaacgatt tgcggccgga gtgcgtttcc
     1561 ccggcttcgt cggtgacgtc aacgctgaga ttgaagatat agtccggtgc cggggtgccg
     1621 tcttccggtt cgtcagcctc ggcaaggaag gatgcggaaa gagatccccc gtcgtcggtt
     1681 accgccgttc ccgttgcgat gactcgcgct gatgtgttaa cggaccgcca ccaccacc
//
LOCUS       DAOPPA010000577         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_74703_length_1731_cov_3.114558, whole genome shotgun sequence.
ACCESSION   DAOPPA010000577 DAOPPA010000000
VERSION     DAOPPA010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74703_length_1731_cov_3.114558"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1480
                     /locus_tag="PKH54_13310"
     CDS             32..1480
                     /locus_tag="PKH54_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00919.1"
                     /translation="MKKLATILAIAAALTMLACGGGSQGTDETTPGDVPGDDAATDEG
                     SSDAINDASPEDLPPVVPDSFQVGYAELDITPPVGTILGGYGVPAMNRVTEGTHDPLM
                     AQAALFTNNAGQALLLISADLAGYMWDFGDWGPGIKVLRQSIVDALAPTMAITPEQIV
                     IASSHSHGAPDLMGFSQEIDEGPDKELLATTITQFTSLAQAAAAALKPATVLYGKTNL
                     EGISARDKDCSPVLDTEVGIIQAQDGQDKVIVTIANFAKHPTIAPESNRLATADFIWG
                     YREEMKKATDAPAMYFQGFEAAVHGRYSFYDEEDFWDKVYEIGKNLADAVLAVSGSLT
                     PAGDFGIQSREATYSCVGRESFMVDAYTYMGLPKRFIDVQQDGTLLVREIPVSWHRLG
                     NAEFVAFPGEPSPEYGVMAKERMSSSFKFALGLANDATGYMVEPQSLANDTTGQLEGY
                     ELKMGLGPDAGPSAWDALQGLGWFDGGWQQEP"
BASE COUNT          367 a          517 c          540 g          307 t
ORIGIN      
        1 gtggttgttt cttccagaag gacactgaaa gatgaaaaag ctggcgacga ttcttgcgat
       61 tgcggccgcg ctgacgatgc ttgcctgcgg gggcggctcg cagggcaccg acgaaaccac
      121 cccgggggac gtgcctgggg atgacgcggc gactgacgag ggctccagcg atgcaatcaa
      181 cgacgcatca cccgaagatt tgccaccggt tgttcccgac agcttccagg tcggttacgc
      241 cgaactcgac atcacccctc ccgttggaac catccttggc ggctacggcg tacccgcgat
      301 gaaccgagtc acggaaggaa cccacgaccc gctgatggcc caggccgccc tgttcacaaa
      361 caacgccggt caggcccttc tgctgatcag cgccgacctg gcgggataca tgtgggactt
      421 cggcgactgg ggtcccggga tcaaggttct gcgccagtcc atcgtcgacg ccctggcgcc
      481 gaccatggcc attacgccgg aacagatcgt tattgccagc agccacagcc acggcgcgcc
      541 cgatctgatg ggcttctcgc aggaaatcga cgaaggcccg gacaaggaac tcctggccac
      601 aacgatcacc cagttcactt cactggccca ggcggccgcc gccgcactga agccggcaac
      661 ggttctttac ggaaagacga acctcgaagg catttcggcc agggacaagg actgctcgcc
      721 ggtgctcgac accgaggtag gcatcatcca ggcgcaagac ggacaggata aggtgattgt
      781 gacaatcgcc aacttcgcga agcacccgac catcgcgccc gagtcgaacc gcctggccac
      841 ggccgatttc atatggggct accgcgagga gatgaagaag gctaccgacg ccccggccat
      901 gtacttccag ggatttgaag cagcggttca tggcaggtac agcttctatg acgaggaaga
      961 tttctgggac aaggtttatg aaatcgggaa gaacctggca gacgcggtcc tggcggtttc
     1021 gggaagcctg accccggcgg gggatttcgg catccaaagc cgcgaagcca cgtattcctg
     1081 cgtcggccgc gagtcgttca tggtggacgc ctatacttac atgggattgc cgaagcgctt
     1141 catcgacgtc cagcaggacg gcacgctcct ggttcgcgag attccggtct cctggcaccg
     1201 cctgggcaat gcggagttcg ttgccttccc cggggagccg tcgccggagt acggcgtgat
     1261 ggccaaggaa cggatgtcca gttccttcaa gttcgccctt ggactggcaa acgacgcgac
     1321 cggctacatg gtggaaccgc agtcgttggc aaatgacacg acaggccagc ttgaaggcta
     1381 tgaactgaag atgggcctgg gcccggacgc cggcccgagc gcgtgggacg cccttcaagg
     1441 ccttggctgg ttcgacggtg gctggcagca ggaaccgtga cacgtttcgg cgttcatgtt
     1501 cgccttcctt ccggtgaagc ctgacttcta ttcgacacca gcccgggtgt tgcccaaatc
     1561 gcgaattact ttagaatgtg gcagttaacc gctccggtaa gaccgcgtgc cgttcgtgcc
     1621 ggaccacaga aaaaggggaa cagatgaaaa agcacaacgc cagggcgatg gtggcagcag
     1681 caattcttat gatgatgacg gcgggatgcg gaagttcgaa tgccgtgcag g
//
LOCUS       DAOPPA010000578         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_75103_length_1725_cov_2.479042, whole genome shotgun sequence.
ACCESSION   DAOPPA010000578 DAOPPA010000000
VERSION     DAOPPA010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75103_length_1725_cov_2.479042"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKH54_13315"
     CDS             complement(<1..1079)
                     /locus_tag="PKH54_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00920.1"
                     /translation="MKIPSIPIVLSEFYQQEKEKALNEGLPGLRTQWIRGPVWAKTRE
                     ELRRDVINGNNPISGKPVMREIVEKFTAPLTAEEQKTGEIIRDKGPATFTDTPEKLQK
                     MFLDNRWTDFLPVVLPTEALVNDMLKGTSHDPDEVVGKMSPTNTGNYFEYWTFTVRDV
                     AVNAVMAGCKPEYLPLLLAAAATGKEAISVSDNSFVGAMVINGNIRDEIGLNSSVGAV
                     GPYAHANTTIGRAWSLIAINLGNCGKVGTTYMGVIGNPMNLINIVIAENEKESPWEPF
                     SVRKGFKKGENVLSLFEGWGILSAANWKASAWGKEMDYPKIIKDIFDQQGFLFGACAV
                     LSPPIANFVRNAGYATVEDFEKFLAP"
     gene            complement(1131..1538)
                     /locus_tag="PKH54_13320"
     CDS             complement(1131..1538)
                     /locus_tag="PKH54_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00921.1"
                     /translation="MKRRLTIAAVTALCIGLFCGFPALDTQAQAKKRAVDPPMPRITV
                     LNPLGTPPPIKLKTMAPRLDTLDGKTIYLVDDGYIGGDNLLYEIQDWFKANHPKTTTV
                     FKRKGGGGFEAEDPALWAEMKEKADGIIIALGH"
BASE COUNT          294 a          534 c          525 g          372 t
ORIGIN      
        1 ggggcgagga atttctcgaa atcctccacg gtcgcatagc cggcgttcct gacgaaattg
       61 gcgatcggcg ggctcaggac cgcgcaggcc ccgaacagaa atccctgctg gtcgaagatg
      121 tccttgatga tcttgggata atccatctcc ttgccccagg ccgaggcctt ccagttcgcc
      181 gccgatagga tgccccagcc ctcgaacagg ctcaggacgt tctccccctt cttgaagccc
      241 ttccggaccg agaaaggttc ccacggggat tctttttcgt tctcggcgat cacgatgttg
      301 atgaggttca tgggatttcc gatgacgccc atgtaagtcg tccccacctt gccgcagttg
      361 ccgaggttga tcgcgatcag gctccaggcc cgcccgatgg tcgtgttcgc gtgtgcatac
      421 ggaccgacgg cgccgacgct cgagttcagg ccgatttcat cgcggatgtt tccgttgatg
      481 accatggcgc ccacgaaact gttgtccgac accgagatgg cctccttgcc cgtggccgcc
      541 gcggccagca ggaggggcag gtattccggc ttgcatccgg ccatgaccgc gttgacggcg
      601 acgtcccgga cggtgaaggt ccagtactcg aagtagtttc cggtgttggt cggcgacatc
      661 ttgccgacga cctcgtccgg gtcgtggctg gtgcctttca gcatgtcgtt gaccagcgcc
      721 tcggtcggca ggacgacggg caggaaatcg gtccagcggt tgtccaggaa catcttctgg
      781 agcttctcgg gcgtgtcggt gaaggtggcc gggcccttgt cgcggatgat ctcgcccgtc
      841 ttctgctcct ccgccgtcag cggcgccgtg aatttttcga ctatttcgcg catcacgggc
      901 ttgcccgaga tcgggttgtt gccgttgatc acgtcgcggc gcaattcctc gcgcgtcttg
      961 gcccacaccg gaccgcggat ccactgggtt cgcagacccg gcagcccttc attcagggcc
     1021 ttttcctttt cctgctggta gaattccgaa agaacgatcg ggatcgaagg gatcttcatg
     1081 ctggtttcga aattgatcga gtggccgaca actgccggcg cacactggct tcaatggccc
     1141 agggcgatga tgatgccgtc ggccttctcc ttcatctcgg cccacagggc cgggtcttcg
     1201 gcctcgaagc cgccgccgcc ctttctcttg aagaccgtgg tggtcttcgg gtgattcgcc
     1261 ttgaaccagt cctggatttc gtacagcagg ttgtcgccgc cgatgtatcc gtcgtcgacg
     1321 aggtaaatgg tcttgccgtc aagcgtatcc aggcgcggag ccatggtctt cagcttgatc
     1381 gggggcgggg tgcccagcgg gttcagcacg gtgatcctgg gcatcggggg atccacggca
     1441 cgctttttcg cctgcgcctg cgtgtccaga gccgggaagc cgcagaacag gccgatgcac
     1501 agcgctgtta cggcggctat cgttaaacga cgcttcatga gctaatcctc ctcgaaaaaa
     1561 cagcatctta ggccggacag attccgaatg tccacaaaga gaacgcggcc ggacaaaaaa
     1621 agtcggcagc aggccgcaaa gcccgctgcc gactgtcgac tgccgactac cgactgctta
     1681 ccgccacttg tcgatggcga tcgcctggcc gggcctcagg ccgtc
//
LOCUS       DAOPPA010000579         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_75384_length_1720_cov_2.608408, whole genome shotgun sequence.
ACCESSION   DAOPPA010000579 DAOPPA010000000
VERSION     DAOPPA010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75384_length_1720_cov_2.608408"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKH54_13325"
     CDS             complement(<1..586)
                     /locus_tag="PKH54_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00922.1"
                     /translation="MLAQLWRDVVVKNVVEKFSPVALKVLSNEKVKNLLTQALKLQSD
                     LKHSADAQVKFVARTFELVTRDELSSVRSVIKTLESEVTRLKAELEVQRKAADDAGKA
                     ARDAFQELNQVKSEAMKATRDAVARTMALKTETIKVARDAVDEATKSSRAVAAEMIRE
                     GLQEAKKTAEEAARKVEAELASQAAALKAAAARAA"
     gene            935..>1720
                     /locus_tag="PKH54_13330"
     CDS             935..>1720
                     /locus_tag="PKH54_13330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD00923.1"
                     /translation="MSRKIRVGINGFGRIGRLVARVMMERGNYEIVAVNDILDPKDFA
                     GSVKQLAMLFKYDSAHGPFKGTVEYNDTQIIINGQATDVVSVKNPEELPWAKYGVDFV
                     VESTGVFRKREQIEGHMKNGGVGRVLLTVPPKDAIDAIIVMGVNDADLKKEHKIISNA
                     SCTTNCLAPVVKVLHDRFGVVRGMMTTIHAYTNDQRILDQFHKSDMRRARSAAANLIP
                     TSTGAAKTIGKIITDLKGKLDGMAVRAPVQDGSLVDFVCELERI"
BASE COUNT          362 a          516 c          479 g          363 t
ORIGIN      
        1 cggcggccct tgctgcggca gccttcagcg cggcagcctg gcttgccaac tcggcctcga
       61 ccttccttgc ggcttcctcg gcggtcttct tcgcttcctg caggccctcg cgaatcattt
      121 cggccgcaac ggcacggctg gactttgttg cctcgtcaac cgcgtcgcgg gcaaccttga
      181 ttgtctccgt cttcagcgcc atcgtcctgg caaccgcgtc cctcgtggcc ttcatcgcct
      241 ccgacttgac ctggttcagt tcctggaaag cgtccctggc cgcctttccc gcatcgtccg
      301 ccgccttgcg ctggacttcg agttcggcct tcagcctggt gacctcgctc tccagtgtct
      361 tgataacgct gcgaaccgag gaaagctcgt ccctggtcac cagttcaaag gtgcgggcga
      421 cgaacttgac ctgggcgtcg gccgaatgct tcaggtcgga ctggagcttc agggcctggg
      481 tcaggaggtt cttgaccttt tcgttggaaa gaaccttcag ggcaaccggt gaaaactttt
      541 caacgacgtt cttcacgact acatcgcgcc acagttgagc aagcatttat tcaatcctcc
      601 agggaagaca tcccagacat ctgacccgac atcaaagaga tcgcaagaat gcgaacgaca
      661 aggctatatt gtttatgacc ttcgaatatg tcaagccgca acggtgcatt gagacaccac
      721 gaccccctgg attgacgtga ccgccccggc cgccgggcgc aatcggcgtt gtcgtgaaat
      781 aaatcacaga aattgtatca gaccgcttcg tttgattgac aaacccgtga ctttacttaa
      841 aatcaggctg ccttcgggga agcacacccc aggcgcttca ggggcaatcc cgccccccgg
      901 attgggcacg aacacccaac gaacaaggag catcatgagc agaaagattc gtgtcgggat
      961 caatggtttt ggtcgaattg gtcgcctggt cgcgcgtgtg atgatggagc gcggaaacta
     1021 cgagatcgtc gccgtcaacg acatcctcga tccgaaggat tttgccggca gcgtgaagca
     1081 gcttgcgatg ctgttcaagt acgactccgc tcatggcccg ttcaagggta ccgtcgaata
     1141 taacgacacc cagattatca tcaacggaca ggccaccgat gtcgtatccg tgaagaaccc
     1201 ggaggaactc ccctgggcca agtacggcgt cgatttcgtc gtcgagtcga ccggcgtgtt
     1261 ccgcaagcgc gagcagatcg aaggtcacat gaagaacggc ggcgtcggtc gcgttcttct
     1321 gaccgtgccg ccgaaggacg cgattgatgc aatcatcgtg atgggcgtca acgacgcgga
     1381 tctgaagaag gaacacaaga tcatcagcaa cgcttcgtgc acgaccaatt gcctggcccc
     1441 cgtggtcaag gttcttcacg acaggttcgg cgtggttcgc ggcatgatga cgacgattca
     1501 tgcgtacacc aacgaccaga ggattctgga tcagttccac aagagtgaca tgcgccgcgc
     1561 ccgcagtgcc gccgccaacc tgattcccac ttcgaccgga gcggcgaaga ccatcggcaa
     1621 gatcatcacc gatctgaagg gtaagctcga tggcatggcc gttcgtgcgc cggttcagga
     1681 tggatcgctt gtcgatttcg tctgcgagct tgagagaatc
//
LOCUS       DAOPPA010000580         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_78604_length_1670_cov_2.694737, whole genome shotgun sequence.
ACCESSION   DAOPPA010000580 DAOPPA010000000
VERSION     DAOPPA010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78604_length_1670_cov_2.694737"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PKH54_13335"
     CDS             <1..1000
                     /locus_tag="PKH54_13335"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844153.1"
                     /note="catalyzes the 4-phospho-L-aspartate from
                     L-aspartate and the formation of lysine from
                     meso-2,6-diaminoheptanedioate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HOD00924.1"
                     /translation="KANGNADVLRVFHEAGLTFECVSPGEIEWLFELFPGLDPTRILF
                     TPNFAPRSEYEFAFEKGVRVTLDNHYPLQAWPELFAGREIFVRLDPGHGRGHHKHVHT
                     AGAHSKFGVFEEELDALGRLVSQAGATVTGLHAHVGSGIFDPDAWRQTALFLACASDR
                     FPDAKILDLGGGLGVPYKPGQKPLDLVAMDAMLGRIKAAFPRFELWLEPGRYLVARAG
                     VLVAPVTQLKQKGEYSYVGISTGMNSLIRPALYGSFHNIVNLSRMDAPLTLTANVVGP
                     ICESGDVLGQDRRLPETFEGDILLIETAGAYGRVMSSEYNRRPPAREVFLPVRQML"
     gene            1033..>1670
                     /locus_tag="PKH54_13340"
     CDS             1033..>1670
                     /locus_tag="PKH54_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00925.1"
                     /translation="MKVKTILLVTSVAFVVGHLVACGDQVGPGDDLGDMDSVGFDVTP
                     EDSGGIDVTPDDRDILAGPLSNGVLLYRNDAGDLVLAHKKRDLLVLPGDVGPVTRKFG
                     QTYESLFGQWRFVRTDVVELPMAPGSVEQGEGLYGHIGDVIVLNFADEAGSVSATLIV
                     ESLEDESTRLYLGSGGLAGDSIAFPIRCGADDTFHGFGEQYNAIDQRGQAFDL"
BASE COUNT          295 a          495 c          542 g          338 t
ORIGIN      
        1 caaggccaac ggaaatgcgg acgtccttcg ggtctttcat gaggccggcc tgacgttcga
       61 atgcgtttcg ccgggcgaaa tcgagtggct tttcgaactc ttcccgggcc tggatccgac
      121 caggatcctt ttcacaccca acttcgcccc gcggtcggag tacgaattcg cctttgaaaa
      181 gggagtccgc gtcaccctgg acaaccacta cccgctgcag gcctggccgg aactgttcgc
      241 cggccgcgag attttcgtgc gcctggaccc gggacacgga cgtggacatc acaagcacgt
      301 ccatacggcg ggtgcccaca gcaagttcgg tgtgttcgag gaggagcttg atgccctggg
      361 gcgcctggtt tcacaggccg gagccacggt caccgggctt cacgcccacg tcggcagcgg
      421 gatattcgac cctgacgcat ggcgacagac cgcgctgttt ctggcctgcg cgtcggatcg
      481 cttcccggac gcgaagatcc tggaccttgg cggcggcctg ggggttccgt acaagccggg
      541 tcagaagccc ctcgacctgg tcgcgatgga cgccatgctt ggacgaataa aggcggcctt
      601 cccgaggttc gaactgtggc tggagcctgg ccgctatctg gtcgcgcggg ccggggtact
      661 ggtcgcgcca gttacccagt tgaagcagaa gggtgaatac agctacgtcg gaatatctac
      721 cggcatgaat tccctgatca ggccggccct ttacggaagt tttcacaaca tcgtcaacct
      781 gtcccgcatg gacgcgccgt tgacgctgac ggcaaacgtc gtcggcccca tatgcgaatc
      841 cggtgacgtg cttggccagg accgtcgcct gcccgagacg ttcgaggggg acatcctgct
      901 gatcgagacc gcgggcgcct atggccgggt gatgtcgtcc gagtacaacc gccggcctcc
      961 ggccagggaa gtgttcctgc cggttcgtca aatgttgtaa tctaggggct gcctgtcccg
     1021 gggaataacc gcatgaaagt caagaccatc cttcttgtga cgtccgtggc attcgtcgtg
     1081 ggtcatctgg tcgcatgtgg tgaccaggtg gggcctggtg acgacctggg cgacatggat
     1141 tcagtcgggt ttgacgtgac cccggaagat tccggcggaa tcgacgtgac cccggacgac
     1201 cgcgacatcc ttgccggccc tttgtccaat ggcgtcctgc tttatcggaa cgacgccggg
     1261 gatctggtac tggcccacaa gaagcgggat ctcctggttc tgccgggcga tgtcgggcct
     1321 gtcaccagaa agttcgggca gacgtacgaa agccttttcg gtcagtggcg ctttgtgcgg
     1381 accgacgtgg tggaactgcc gatggcgccg ggttcagtgg agcaggggga gggcctttac
     1441 gggcacattg gcgatgtgat cgtgctcaat tttgctgatg aggccggttc ggtgtccgca
     1501 acactcatcg ttgagtcact cgaggatgaa agtacccgcc tttaccttgg atcgggcggg
     1561 ctggccgggg acagtatcgc ttttccgata aggtgcggcg ccgacgacac tttccatggc
     1621 ttcggcgagc agtacaacgc catcgaccag cgcggccagg cgttcgacct
//
LOCUS       DAOPPA010000581         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_78654_length_1669_cov_3.507435, whole genome shotgun sequence.
ACCESSION   DAOPPA010000581 DAOPPA010000000
VERSION     DAOPPA010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78654_length_1669_cov_3.507435"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PKH54_13345"
     CDS             <1..110
                     /locus_tag="PKH54_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD00926.1"
                     /translation="DRAGQFSIRVNDRWRICFEWSGGTAEHVELVDYHD"
     gene            120..428
                     /locus_tag="PKH54_13350"
     CDS             120..428
                     /locus_tag="PKH54_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOD00927.1"
                     /translation="MTGKMPPVHPGEILVEEFLNPLGVSQYRLAKAIGVPARRINEIV
                     HGIRGITADTALRLGRYFGTSPEFWMNLQARYDLEVRREQLGSLLTTGIPVFAASQRP
                     "
     gene            complement(494..1222)
                     /locus_tag="PKH54_13355"
     CDS             complement(494..1222)
                     /locus_tag="PKH54_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOD00928.1"
                     /translation="MTTTLIPKWLEPFSQPVQDAIMLLMAEPGRKLACFDADGTLWNE
                     DIGEAFFRWLIAGNLLPHRDCTRDIWQEYEDRVAENRAQGYAWAVQSMVGIPESDIVR
                     WSIQMAAAWPNYRPAMADLARGLAESGFDVWIVSATNQWTVRAAAPKMNFDPDNVIAM
                     ASAPEGGIMTETMVEPLICNAGKVTAIQQKFGRLPDVSFGDSMGDFEMLCASRVPVVI
                     GRNDKPNRAILAEAVTRGWPVHMF"
     gene            1558..>1669
                     /locus_tag="PKH54_13360"
     CDS             1558..>1669
                     /locus_tag="PKH54_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD00929.1"
                     /translation="MDGTVYLGKRLLPGTPPFLQMCRDRGIPSVFLTTNSS"
BASE COUNT          333 a          452 c          556 g          328 t
ORIGIN      
        1 cggacagggc ggggcagttc agcatcaggg tgaatgacag atggcggatc tgtttcgaat
       61 ggtccggagg aacagcggaa cacgttgagc ttgtggacta tcacgactga ggtgcttaaa
      121 tgactggaaa aatgccgccg gtgcatccgg gagagatact ggtcgaggaa tttctgaatc
      181 cgctgggcgt ttcgcagtac aggcttgcga aggcaatcgg cgtgccagcg cggcgcatca
      241 atgaaatcgt tcatggaatc cgcggaatca cagcggacac ggccctgaga ctggggcgtt
      301 acttcggcac ttcccccgaa ttctggatga acctccaggc ccgttacgat ctggaagttc
      361 gccgtgaaca actcggcagc ctgctgacga ccggcatccc ggtcttcgcc gcgtcgcaaa
      421 gaccgtgaat ttgaaccaaa aacgtgacgg tttgaaatgg aagtccttcc gacagggcgg
      481 ttccgcgcgg cgatcagaac atatggacgg gccagccgcg ggtcacggcc tcggccagga
      541 tggcgcggtt gggcttgtcg ttgcgaccga tgacgaccgg cacgcggctg gcgcacagca
      601 tttcgaagtc gcccatgctg tcaccgaagg aaacgtcggg caggcggccg aatttctgct
      661 gtattgccgt aaccttgccg gcgttgcaga taagcggttc gaccatcgtt tcggtcatga
      721 tgccgccctc cggggcgctt gccatcgcga tgacattgtc ggggtcaaag ttcatcttcg
      781 gtgccgcggc gcggacggtc cactggttgg tggcggatac gatccagacg tcgaagccgg
      841 attcggccag cccgcgggcc aggtcggcca tggctgggcg gtagttgggc caggcggccg
      901 ccatctggat tgaccagcgg acgatgtcgg attcggggat gccgaccatg gactggacgg
      961 cccaggcgta accctgggcg cggttttcgg cgacccggtc ctcgtattcc tgccagatgt
     1021 cgcgcgtgca gtcgcgatgg ggcagaaggt tgccggcgat cagccacctg aagaaggcct
     1081 cgccgatgtc ttcgttccac agggtcccgt ccgcgtcgaa acaggccaac ttgcggccgg
     1141 gctcggccat cagcagcatt atcgcgtcct gaaccggctg ggaaaacggt tcgagccatt
     1201 tcggaatcag tgttgtcgtc atgtgcttat atccagcgca aggccagaca attacgccgg
     1261 agcgacgcgc gtgtgtcaat gccaattgtt cgggcgaatg atcacgtcga agcttcagag
     1321 tgggcgggtc atctgggcat aagagggcaa agtgacatgg gcgaattttc attcgccctt
     1381 acgggggaag ggccggaagg gtgaaacgcc atgggtggac tttaatgcga ccctgccgtg
     1441 acggttcgtg tacaatcgcg gcgtttccgg gggcttgctg atgcggggac gaatatgatt
     1501 gaaatcacga agggtgcggc ggaaagaata aagaacatca aggcgttcgc gctggacatg
     1561 gatggaacgg tttacctggg gaagcggctt ctgccgggaa ccccgccgtt tctgcagatg
     1621 tgccgcgatc ggggcattcc gtccgtgttc ctgaccacca acagctcga
//
LOCUS       DAOPPA010000582         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_78927_length_1665_cov_3.326708, whole genome shotgun sequence.
ACCESSION   DAOPPA010000582 DAOPPA010000000
VERSION     DAOPPA010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78927_length_1665_cov_3.326708"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKH54_13365"
     CDS             <1..542
                     /locus_tag="PKH54_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD00930.1"
                     /translation="FPADFLLIAAMNPCRCGYLGDSRHECSCTTTEIARYRSRVSGPL
                     LDRIDLQVEVSPVPFRELSAATGRGTSGEAAERVLAAAKIQADRFKGSAVLFNGRMDS
                     PGVRRNCAVPPEAETLLEHSMDRLGLSARALDRILKVARTIADLDLCERIRTEHVAEA
                     IQYRMLDRLKGQFPQAFPA"
     gene            567..1610
                     /gene="recA"
                     /locus_tag="PKH54_13370"
     CDS             567..1610
                     /gene="recA"
                     /locus_tag="PKH54_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOD00931.1"
                     /translation="MAKQEVIAVHELDKLIANMERQFGKGAVMRYGDGPVEKVPVFST
                     GSISLDRALGIGGIPKGRIVEVYGPESSGKTTLTMEIIAQAQKTGAVAAFIDAEHAFD
                     IGYARNLGIRVEDLLVSQPDYGEHALDIAETLVRSGSIDLVVVDSVAALVPKAEIEGE
                     MGDSHVGLQARLMSQALRKLVGLCYKQDCTVIFINQLRQKIGVMFGNPETTTGGNALK
                     FYSSIRLDVRRKGSQKDGAVVTANRVRVKVAKNKMAPPFLEAEFDIVFGEGINRTLDL
                     LEAAEAAGLIEKSGSWLSFGGEQLGQGRDQADRHIRADDNLRAALESAVRGTAPTRSG
                     RAVEPPEEESDVA"
BASE COUNT          380 a          468 c          512 g          305 t
ORIGIN      
        1 catttcccgc cgacttcctg ctcatcgccg cgatgaatcc ttgtcgctgc ggatacctcg
       61 gggattcccg acatgagtgt tcgtgcacga ccaccgaaat cgcaaggtac cgttccaggg
      121 tatccggccc gctgcttgat cgcatcgacc tgcaggtcga agtgtcaccc gttccatttc
      181 gcgaactttc ggcggcaacc gggcgcggaa cgtcagggga agctgccgaa cgggtgttgg
      241 ccgcggcaaa aattcaggcc gacaggttca aaggatccgc ggtgcttttc aacgggcgga
      301 tggactcgcc tggtgtccgc cgcaactgtg cggtaccgcc cgaggccgaa acgcttcttg
      361 agcactcgat ggacaggctg gggctgtcgg caagggcgct tgatcgaatc ctgaaggtgg
      421 caaggacgat cgccgatctg gacctgtgcg aaagaatccg cacggaacac gtggccgaag
      481 cgatccagta caggatgctt gatcgcctga agggacagtt cccccaggca tttcccgcat
      541 agcagtcatt taacggagga aagaccatgg caaaacagga agtgatcgcg gtgcacgaac
      601 ttgacaagct tatcgccaac atggaaaggc aatttgggaa aggtgccgtc atgcgttacg
      661 gcgacggacc ggtggaaaag gttccggtat tttcgaccgg cagcatttcg cttgaccggg
      721 cactcggaat cggcggaatc cccaaaggtc gcatcgtcga agtgtatggc cccgagtcat
      781 ccgggaagac aaccttgacg atggaaatca tcgcccaggc ccagaagacc ggcgcggtgg
      841 cagcctttat cgacgccgag cacgcatttg acatcgggta cgccagaaat ctgggaatcc
      901 gtgtcgaaga cctgcttgtc agccagcccg attatggcga gcatgccctg gatatcgccg
      961 agactctggt tcgaagcggc tcaatcgacc ttgtggttgt cgattccgtg gccgcactgg
     1021 taccaaaggc cgaaatcgaa ggtgaaatgg gcgacagcca cgtcggcctt caggccagac
     1081 tgatgtccca ggccctgcgc aagctggtcg ggctgtgtta caagcaggac tgcacggtca
     1141 tcttcatcaa ccagctcagg cagaagatcg gggtgatgtt cggaaacccc gaaacgacca
     1201 ctggcggaaa cgccctgaag ttctactcgt ccatccggct ggacgtaaga aggaagggat
     1261 cccagaagga tggcgccgtt gtgacggcca accgggtcag ggtcaaggtg gccaagaaca
     1321 agatggcacc cccattcctc gaggccgagt tcgatatcgt tttcggcgag ggaatcaacc
     1381 gaacgcttga cctgcttgaa gcggccgaag ctgccggcct gatcgaaaaa tcgggatcat
     1441 ggctgtcgtt tggtggcgaa cagcttggac agggacgcga ccaggccgat cgacatattc
     1501 gtgccgatga caacctccgg gctgcattgg aatcggccgt gcgtggaacg gcccccacca
     1561 gatccggacg tgcagtcgaa ccccccgaag aagaaagcga cgtcgcatga atccggacag
     1621 aatcagacga tgaacgggat aagcgccttg cggttcttcg gatag
//
LOCUS       DAOPPA010000583         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_79398_length_1658_cov_6.756082, whole genome shotgun sequence.
ACCESSION   DAOPPA010000583 DAOPPA010000000
VERSION     DAOPPA010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79398_length_1658_cov_6.756082"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PKH54_13375"
     CDS             <1..145
                     /locus_tag="PKH54_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD00932.1"
                     /translation="DSVTVYTTGREKFPTNIIAGMFNFGPAELLESTESPEERKAPRV
                     SFN"
     gene            176..>1658
                     /locus_tag="PKH54_13380"
     CDS             176..>1658
                     /locus_tag="PKH54_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD00933.1"
                     /translation="MDFFAQQDMARKKSGRLVVLFILAVVFIILAVYVVVLALGFFIG
                     PPGDVKAPEQIVWQWWRPNILFTVTFMVILAVGSAALMKLAALRKGGAFIAQSLGGVE
                     VSSTSSDPDERRLMNVVEEVAIASGVPVPSVFILPGEAGINAFAAGWSPSDAAVAVTT
                     GAMKQLTRDELQGVVAHEFGHILNGDMRLNLKLVAMISGIMVLATIGYWALHVRPSRN
                     SKNGNGYAVILLAGLALLAIGYGGAFVGRIIQSAVSRQREYLADASAVQFTRNPAGLA
                     NALKRIAGYPVGSVMRAPAAREMRHMFFGASDVVSFFGSMFATHPPLNQRIKQLDPSF
                     DGDVSRLSVADGDAGPAGVAGFAGQSGYPALTGAQAVSGIGTLTFEHVQAGSALISMI
                     PPRIRDEIEDPLGACAVVSAMMLDKDQSIRQRQIEAIATQFSEALARQTEILAADVAG
                     LDRQLLLPIVDLSLPAMRRMSPVQYVSYSKTVASLARADNRVTL"
BASE COUNT          283 a          510 c          512 g          353 t
ORIGIN      
        1 cgactcggta acggtttaca ccaccggccg cgagaagttt cccacgaaca tcatcgcggg
       61 gatgttcaac ttcggaccgg cggaactgct tgaatcgacc gaatcgcccg aggaacgcaa
      121 ggcgcccagg gtctcgttca actgacgtct ccaactcttg ccaaatggaa tattcatgga
      181 cttcttcgcc cagcaggaca tggcccgcaa gaagagcgga cgacttgtcg tccttttcat
      241 cctcgcagtc gtgttcatca ttctggccgt ctatgtagtc gtcctggccc ttggtttctt
      301 catcgggccg ccaggcgatg taaaggctcc ggaacagatc gtctggcagt ggtggcgccc
      361 gaatatcctc ttcaccgtga cattcatggt gatcctggct gtcgggagcg ctgccctgat
      421 gaaactggcc gcactgcgga aaggtggcgc gttcatcgcc cagagtcttg gcggcgtaga
      481 ggtttccagc accagcagtg atcccgatga acgccgcttg atgaacgtcg tcgaggaggt
      541 tgccatcgct tccggcgttc cggttccgtc ggttttcatc cttcctggtg aggctggaat
      601 caatgccttt gccgcaggtt ggtcgccgtc cgacgcggcg gtggccgtca cgaccggggc
      661 catgaagcaa ctcacccgcg acgagctcca gggggtcgtg gctcatgagt tcgggcacat
      721 cctgaacggc gacatgcgtt tgaatctgaa gctggtggcc atgatttccg gaatcatggt
      781 tctggcgacc atcgggtact gggccctgca tgtccggccg tcacgcaact ccaagaatgg
      841 taatggctat gcagtgatcc tgcttgccgg gctggctctt ctggcgattg gctacggcgg
      901 cgcgttcgtc ggccgtatca tccagagcgc ggtttccaga cagcgtgagt accttgccga
      961 cgccagcgcc gtgcaattca cccgcaatcc ggcgggactg gccaacgccc tcaaacgcat
     1021 cgccgggtat cccgtcggca gcgtgatgcg cgcgccggct gcccgcgaga tgaggcacat
     1081 gttcttcggt gcctcggatg tcgtgtcctt cttcgggtcg atgttcgcga cacatccacc
     1141 gttgaaccaa cgcatcaaac agctcgatcc gtcgttcgac ggcgacgtgt cgcggctatc
     1201 tgtcgcggat ggggatgccg ggccggccgg cgttgccggg ttcgcggggc agtccgggta
     1261 tcccgcgttg accggagccc aggcggtatc gggaatcggc acgctgacgt tcgagcacgt
     1321 tcaggccggt tccgctttga tctcgatgat tccacctcgt atccgcgacg agatcgagga
     1381 cccgctggga gcgtgcgcgg tggtgtcagc gatgatgctc gacaaggacc agtcgatccg
     1441 ccagcgtcag atcgaggcca tcgcgacgca gttttcagag gctcttgccc ggcagaccga
     1501 gatactggcc gccgatgtcg cgggactcga ccgtcaactg ttgctgccga tcgtcgacct
     1561 ttccctgcct gcgatgcgcc ggatgtcgcc tgttcagtac gtatcctact cgaagacggt
     1621 cgcatccctg gcccgggccg acaatcgggt aaccctgt
//
LOCUS       DAOPPA010000584         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_79429_length_1658_cov_2.472863, whole genome shotgun sequence.
ACCESSION   DAOPPA010000584 DAOPPA010000000
VERSION     DAOPPA010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79429_length_1658_cov_2.472863"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKH54_13385"
     CDS             <1..272
                     /locus_tag="PKH54_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00934.1"
                     /translation="GAGVFGHYEDHLCLTAPGRRRGFWSLPGWFYPIDEGRTPLGYHR
                     NPDLWSREGDNAQLKTMARGQEFVLDADQYPEVGAWVRGLLEKSV"
     gene            292..807
                     /locus_tag="PKH54_13390"
     CDS             292..807
                     /locus_tag="PKH54_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00935.1"
                     /translation="MSRVKFGISNDSADYESLRTLFQNIDLVLRHRDTILNTPDLCNI
                     SVAGCGTFLLYVGPIRLSLGDLVWLWNNECFWLEREGDDTRYAFCAVGSPLSGRHAMH
                     CFSTLAGDFKCLRRGRGAVVSLLDVTRCRTQRGLVRPDDDVPTLRASLPPASALTVDY
                     LIAALKSIEKT"
     gene            complement(843..1523)
                     /locus_tag="PKH54_13395"
     CDS             complement(843..1523)
                     /locus_tag="PKH54_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00936.1"
                     /translation="MSIQNETAALNVKPFATDLEYLTAELAWVHARTRRIEASQLTLE
                     QDPVLARVQSGRVRAVYTDATASMAGQLAREELAIRQEIDARLALNRAGGLGLGLDRL
                     CLEHGLGQFERTVLLLATIPCIGYSMLETHVAPLRPRYNSTMIEANLVSLFLELDMKG
                     GLEGLLCLLPDAPLRKAGLLVMTYEPRTPASALDVGFELTGKSLAAITGIPAFEGFAD
                     PDLLPDVD"
BASE COUNT          320 a          497 c          498 g          343 t
ORIGIN      
        1 ccggtgcggg agtgttcggc cattatgaag accatctttg cttgacggct cctgggcggc
       61 gtcggggctt ctggtccctg ccgggttggt tttacccgat cgacgagggg aggacgccac
      121 tgggttacca ccgaaacccg gatctctggt ctcgggaagg cgacaatgct cagctcaaga
      181 ccatggcacg cggtcaggaa tttgtgcttg atgctgacca gtaccccgag gttggtgcat
      241 gggtgcgcgg gttactcgaa aagagtgttt gactcaagtg aagtccttcc aatgtcacgt
      301 gttaagttcg gcatttccaa cgattcggct gactacgaat cccttcgaac cctgttccag
      361 aacatcgacc ttgtccttcg acaccgcgac acaatcctga acacgccgga cctgtgcaac
      421 atcagcgtgg ccggctgtgg gacgttcctg ctttacgtcg gcccgatccg gctttcactt
      481 ggtgacctcg tctggctgtg gaacaacgaa tgcttctggc tggagcgtga aggcgacgac
      541 acaaggtatg ccttctgcgc cgtgggcagt ccgctgtccg ggagacacgc gatgcattgc
      601 ttcagcacgc ttgccgggga tttcaagtgt ctccgccgtg ggcgtggcgc ggtcgtttca
      661 cttcttgacg tgacgcgctg tcgaacgcaa cgtggccttg tcaggcccga cgatgacgtc
      721 cccacactac gggccagcct acccccggca tccgccttga cggtcgacta tctgatcgcg
      781 gccctgaaaa gcatcgagaa aacctgaagc cctgttcatc cggccaatga attccggcgg
      841 cttcaatcga catcggggag caggtcggga tcggcgaagc cttcgaaggc cggaatgcca
      901 gtgatcgcgg caagcgactt gccggtcagt tcaaagccga catcaagagc ggaagccggc
      961 gtccggggtt cgtacgtcat caccaacagc ccggccttgc ggaggggcgc gtcgggcaga
     1021 aggcacagca ggccttcgag gccgcccttc atgtccagtt caaggaaaag cgacacgagg
     1081 ttggcctcga tcatcgtcga gttgtaacgc ggcctcaggg gcgccacgtg cgtttccaac
     1141 atggaatatc cgatgcacgg aatcgtggcc agcagaagga cggtgcgttc gaattgtccc
     1201 agaccgtgct ccaggcagag gcgatccagc cccagcccca gaccacccgc gcggttcaga
     1261 gccaggcggg cgtcgatttc ctgccggatg gccagctctt cccgggcaag ctggccggcc
     1321 atgctcgccg tcgcgtcggt gtacacggca cgcacccgtc ccgactgcac cctggcaagc
     1381 accgggtcct gttccagggt cagctggctc gcctcgatcc gcctagtgcg ggcatgcacc
     1441 cacgccagct cggcggtcag gtattccaga tcggtcgcaa agggtttcac gttcagggcc
     1501 gctgtttcgt tctggatgct catttttacc tcctgtggtt ttgagttcat ccagtacgtc
     1561 tactggggag ttcggagatc taagccggaa atctttggaa tcctggaaaa aagttcaaac
     1621 aaacgcattt aatcatgcgg atttcgccgc aacaaaag
//
LOCUS       DAOPPA010000585         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_79670_length_1654_cov_3.648530, whole genome shotgun sequence.
ACCESSION   DAOPPA010000585 DAOPPA010000000
VERSION     DAOPPA010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79670_length_1654_cov_3.648530"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKH54_13400"
     CDS             <1..1066
                     /locus_tag="PKH54_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00937.1"
                     /translation="SDFDNGFPVDIVIPVDVPSDVIGVDTAVPDQGGRDQGQQDPGGS
                     GDTVVHRDLPSLDLPDTECGVTLDNYFDMLIDALAGFYARCSPGDRINFWQNPKNARW
                     MLEGQIRPMEVEYRVRIDKGALRLDKTVACAYLGLLKDGDCNATRTMNSGLVGTAGPG
                     GACGVDEECPDGYFCRFPNATTCLGECTARVGAGQICDFGGCQPGFACRLNDADEFRC
                     VASYYIRQPGDDCDPETDVCAQSFCDGSLCHELPGPGTQCSPGSYCRDGWCDRNLNIC
                     KPYVGAGGSCETWTSCEDGMYCLPFPDEKCATRLTLGAQCDPARNSFDFPGVSLACAS
                     GLCDKLNNVCTDSAPASACN"
     gene            1089..>1654
                     /locus_tag="PKH54_13405"
     CDS             1089..>1654
                     /locus_tag="PKH54_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00938.1"
                     /translation="MRFHLAIPAIFAVASLCFVTGCSGGGVAVDAPGADDSEWFDVVS
                     DEGTEDAGIEDAGLDARPDQGGQDVGEADNGQGESYPNACRGFDQPSGTSCTGTSAVG
                     CCDDLGRVVWCQDNSLYCFDCNLNPKDSQSCGWRVVEKFAGYDCGGSGADPAGIFPLE
                     CGDTCIPDCKGRDCGSDGCGGYCGTCGEG"
BASE COUNT          300 a          465 c          556 g          333 t
ORIGIN      
        1 gtccgatttc gacaatggtt tcccggtcga tatcgtcatt ccggtcgatg tgccgtccga
       61 tgtgatcggc gtggacaccg ccgttccgga ccagggtggt cgcgaccagg ggcagcagga
      121 tcccggtggg tcgggcgaca cggttgttca cagggatctg ccatctcttg atcttcccga
      181 caccgaatgc ggggtcaccc tggacaacta tttcgacatg ctcattgacg cgctggccgg
      241 cttttatgca aggtgcagcc ccggcgaccg catcaatttc tggcagaatc cgaagaatgc
      301 ccgctggatg ctcgagggcc agatacgacc catggaagtg gagtaccggg tcagaatcga
      361 caagggcgcc ctgagactgg acaagaccgt tgcatgcgca tacctggggc ttttgaagga
      421 cggggactgc aacgcgacca gaacgatgaa cagcgggctt gtcgggactg ccgggccagg
      481 tggcgcctgc ggcgtcgacg aggaatgtcc ggacggttac ttctgtcgtt tcccgaatgc
      541 gacgacgtgc ctgggtgaat gcacggcaag ggtcggggcc ggtcagattt gtgatttcgg
      601 cggatgccag cccggttttg cctgcaggct gaacgatgcc gacgaattca ggtgcgtggc
      661 ctcgtattac atcaggcagc ccggcgacga ctgcgacccg gaaacagacg tctgtgcgca
      721 gagcttctgt gatggaagct tgtgtcacga gcttcccggc ccgggaaccc agtgttcacc
      781 cggttcatac tgccgcgacg gctggtgtga caggaacctg aacatctgca agccgtacgt
      841 cggcgccggg ggaagctgtg aaacatggac ttcctgcgag gacggcatgt actgtcttcc
      901 gttcccggac gaaaagtgcg ccaccaggtt gaccctgggc gcccagtgcg acccggccag
      961 gaattctttc gactttcccg gcgtgtccct ggcctgcgcc agcggccttt gcgacaagtt
     1021 gaacaacgtc tgcaccgatt ccgcgccggc atcggcgtgt aactgaagtc cccgcatcgg
     1081 aggttccaat gaggtttcat ctcgcaattc cggcaatttt cgctgtcgcg tcgctgtgct
     1141 tcgttaccgg gtgttccggt ggcggcgttg cggtcgatgc gcctggggcg gacgattcgg
     1201 aatggttcga cgtggtatcc gatgaaggca ctgaagatgc cgggatcgaa gatgcagggc
     1261 tcgacgccag gccggatcag gggggtcagg acgtcggtga agccgataac gggcagggtg
     1321 aatcatatcc caacgcatgc cggggcttcg accagccgtc ggggacttcg tgcaccggaa
     1381 ccagcgcggt cggttgctgc gacgaccttg gccgggtggt ctggtgccag gacaactcgc
     1441 tgtactgctt tgactgcaac ctgaatccga aggacagcca gtcgtgcgga tggcgtgtcg
     1501 tggaaaagtt cgccggatac gattgcggcg gcagcggtgc cgatccggca ggcatattcc
     1561 cccttgaatg cggcgatacg tgtattcccg actgcaaggg ccgcgactgc ggttcggacg
     1621 gctgtggcgg ttattgcggc acttgcggtg aggg
//
LOCUS       DAOPPA010000586         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_79824_length_1652_cov_3.285535, whole genome shotgun sequence.
ACCESSION   DAOPPA010000586 DAOPPA010000000
VERSION     DAOPPA010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79824_length_1652_cov_3.285535"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKH54_13410"
     CDS             complement(<1..473)
                     /locus_tag="PKH54_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOD00939.1"
                     /translation="MGLRNTLFRLVPAAVLMAVGLVSCAGDDGWPDSGPDSTPGFDVP
                     GEFVSDLDQPDLIDSDQFPAELPGQDGSVPDSDAPDSGPTYPLAVDSMAPRQGDPATS
                     TMVTLTGSGFSAVVDVWFGQARSPSVFAISDGILNCVAPPARSGSVQVIVEDSLGR"
     gene            728..>1652
                     /locus_tag="PKH54_13415"
     CDS             728..>1652
                     /locus_tag="PKH54_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00940.1"
                     /translation="MKEFAKLFGVMVLAVALTGLVACGGDNNEGTDEGNPDTTEQDVN
                     YPEDTNTPDTNRPDEGGVDTNVPPDDVPEEVCTPVCAPNTCVDDGCGNLCACDENFTC
                     NAGTCEPAVCEPKCDGKECGDDGCEGTCGTCDDGFVCVELTGACEAECNYPDDLPTTW
                     GETGVVNSLHVPANATEKEPCFDYTNDGVGDCGLTGLAGQVNGPLNDMMADGTLAIVF
                     EFDSVTDFANTASFTLKGLMGEPVVEGTLAGDMYVDQASYLEETCTPMIKFADASITA
                     GALMAGPGNFTISVPLDENLLITVHLVDAQIK"
BASE COUNT          375 a          530 c          479 g          268 t
ORIGIN      
        1 cggcccaacg aatcctcgac aatcacctga acgcttcccg accgggcggg cggagccacg
       61 caattcagga tgccatcgga aatcgcgaag acggacggac tgcgcgcctg gccgaaccac
      121 acatcgacaa ccgccgaaaa cccgctgccg gtgagcgtca ccatagtaga tgtcgccggg
      181 tcgccctgcc ggggggccat cgaatcgacg gcgagcggat acgtcgggcc ggaatcgggg
      241 gcgtcggaat cagggacgct gccgtcctgg cctggcaatt ccgccggaaa ttgatcggaa
      301 tcaatcaaat ccggctggtc aagatccgaa acaaattcgc cgggcacgtc aaaacctggg
      361 gtggaatccg ggccggagtc gggccacccg tcatccccgg cgcaggaaac cagcccgacg
      421 gccatcagga ccgccgcagg taccagccga aacagggtat tgcgaagccc catcgaagca
      481 ccggaacaca agacgacata ttctagcaaa ctttcgcgac agccggcggc acttccgacg
      541 acgctcgcga cgacgcttcc gtccggcccg aatccaatga gcgcaagccg ccaatgcccc
      601 ggcgccattc agaatgtttc gcgcgccccc aaccaaattg cttgcattcg aacgaaaatt
      661 caaataaact cccggtggct ttaatggcca gtagggttgt tttttattcc attggaggaa
      721 atcaggaatg aaggaattcg cgaagctttt tggagtgatg gttctcgctg tcgccctcac
      781 cggccttgtc gcctgtggcg gtgataacaa cgaaggaacc gacgaaggga acccggacac
      841 caccgaacag gacgttaatt acccggaaga caccaacacg ccggacacca accgcccgga
      901 tgaaggtggc gttgacacga acgttccgcc ggacgatgtt ccggaagaag tctgcacccc
      961 cgtgtgcgct ccgaacacct gcgtcgatga cggctgcgga aacctctgcg cctgcgacga
     1021 aaacttcacc tgcaacgccg gcacctgtga accggcggtc tgcgaaccga agtgcgacgg
     1081 caaggaatgt ggcgacgacg gctgcgaagg cacctgcggc acctgcgacg acggattcgt
     1141 ctgcgtggaa ctgaccggcg cctgcgaagc ggaatgcaac tacccggacg acctgcccac
     1201 gacctggggc gagaccggcg ttgtgaactc cctgcacgtc ccggcgaacg cgaccgaaaa
     1261 ggaaccctgc ttcgactaca ccaacgacgg cgttggcgac tgtggtctga ccggtctggc
     1321 cggccaggtc aacggcccgc tcaatgacat gatggccgac ggcacactgg cgatcgtgtt
     1381 cgaattcgac agcgtcaccg acttcgccaa caccgcttcc ttcaccctga agggcctgat
     1441 gggcgaaccg gttgtcgaag gcacgctggc tggcgacatg tatgttgacc aggcttcgta
     1501 cctcgaagaa acctgcaccc ccatgatcaa gttcgccgat gccagcatca ccgccggtgc
     1561 gctgatggcc ggtcccggta acttcacgat ctccgttccg cttgatgaaa accttctgat
     1621 cactgtccac ctggtcgatg cccagatcaa gg
//
LOCUS       DAOPPA010000587         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_80481_length_1642_cov_3.509767, whole genome shotgun sequence.
ACCESSION   DAOPPA010000587 DAOPPA010000000
VERSION     DAOPPA010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80481_length_1642_cov_3.509767"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PKH54_13420"
     CDS             <1..902
                     /locus_tag="PKH54_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD00941.1"
                     /translation="SAAMDATRKAWLFLFVGIVAASQSGNLVRIGNASPFAITTWRLA
                     LASLMLAPLAGRHLVQLARLGRRDMILLLGAGIALALHFFSWIAAVQHTTVANASIFF
                     SINPVITALGGWLFYREMPSRRLALSIALGLGGVLFIGISDLDFAPERFAGNALSVLS
                     SLLFTLYFLLGKRVRQTVDSRAYVTGVYGVAAMTGLLFVMLLGQPVFDYSARNWTCFA
                     LMALIPTMIGHTSLNHGLRYIQAGRISAAMLLEPLTAGLGAAIFWGESIQPHTIGGYV
                     LICVSVLVLILEPTARKNATNSR"
     gene            complement(1327..>1642)
                     /locus_tag="PKH54_13425"
     CDS             complement(1327..>1642)
                     /locus_tag="PKH54_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD00942.1"
                     /translation="SSGGDGCGQDRTWPVCSKVAGNTAQGLCDMAGNVWEWVSDWYES
                     GYYGKSPSLNPTGPSSGSARVDRGGSRYNGAGVVRAAFRGRNVPGRRLGDLGFRVARS
                     LP"
BASE COUNT          317 a          516 c          479 g          330 t
ORIGIN      
        1 attcggcggc catggacgcg acccgcaaag catggttgtt tctgtttgtc ggcatcgtgg
       61 cggcaagcca gtcaggcaac ctggtccgca tcggcaacgc atcgccgttt gcaatcacga
      121 cctggcggct tgcacttgcc tcgctgatgc tggcgccatt ggcgggtcga cacctggttc
      181 aactggcccg ccttggccgg cgcgacatga tcctgctgct tggggccgga atcgccctgg
      241 cactgcactt cttttcatgg atcgcggcgg ttcagcacac gaccgttgcc aacgcatcca
      301 tcttcttttc gatcaacccg gtaatcaccg cgctgggcgg gtggctgttt taccgcgaga
      361 tgccttcgcg gcgccttgca ctgtcaatcg cgctcgggct gggaggcgtc ctgttcatcg
      421 ggatctccga cctggatttc gcgccggaaa ggtttgccgg aaacgccctg tcggtgcttt
      481 catcgctgct tttcaccttg tacttcctgc ttgggaagcg cgtgcgacag acggttgaca
      541 gccgtgcata cgtgaccggg gtgtatgggg tcgcggcgat gaccgggctg ctgttcgtga
      601 tgctgctggg ccagccggtg ttcgattatt cggcgcgcaa ctggacgtgc tttgcgttga
      661 tggcactgat tccgaccatg atcggccaca cgtcgctgaa ccacgggctt agatacatcc
      721 aggccggacg tatctcggca gccatgcttc ttgaaccact gaccgccggc cttggcgccg
      781 ccatcttctg gggcgaatcg atccagcccc acaccatcgg tggctatgtc cttatctgcg
      841 tatccgtgct tgtcctgatt ctggaaccga ccgcccggaa aaacgcaacg aattctcggt
      901 aggggcgaat gataattcgc ccggcccagc aacccgtccc gtaagggcga atgaaaattc
      961 gcccgcccgc aaaaaatcgc gcggcgcccg ggctggtgcg gcgatgcttt cggggccgtg
     1021 gcccatgcgg gccgccccga ctttcgacag cggtcattcg gcccatacag caatagggtc
     1081 aatcgaaaat tcaccccaac attttggatt cccatttacg gcgtattcgg gaacgggccg
     1141 cgtggcgacg ttgagaaaat ttgcggtcgc ggcccatggg ccgcgacatg tcggaccatg
     1201 tcattcacca atcttgtcgc ggggccgtat gcgagggcta tggcaagcca gcccccgcac
     1261 ggcccacttc aaacattcag aaaattccga cggaaaaggg taaaagggta aaagaaataa
     1321 gggaaattaa ggcaaggacc tggcgacgcg aaagccgagg tcgccgaggc ggcggccagg
     1381 gacgttgcgg ccgcgaaagg cggcacggac gaccccggcg ccattgtacc tcgacccgcc
     1441 acgatcgacc cgggccgagc ctgatgaagg accggtcgga ttcaatgacg gactcttccc
     1501 gtaataacct gattcgtacc agtcagatac ccactcccat acgttaccgg ccatatcgca
     1561 caagccctgc gccgtattac ccgcaacctt cgaacacacc ggccacgtcc gatcctgacc
     1621 acatccgtca ccaccagaac tg
//
LOCUS       DAOPPA010000588         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_80697_length_1639_cov_3.020202, whole genome shotgun sequence.
ACCESSION   DAOPPA010000588 DAOPPA010000000
VERSION     DAOPPA010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80697_length_1639_cov_3.020202"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKH54_13430"
     CDS             <1..594
                     /locus_tag="PKH54_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD00943.1"
                     /translation="SYVNKLCPSIHPSDAITAFAGLRAVSSTGDFIIGPTSVKGFINA
                     AGIQSPGLTSAPSIGEYVVGILESEGLSMSLKDDFRAEIDGPPRFAKQPVDVQERMVA
                     SDPAFGKIVCRCEIVTEAEIHDAIDHGARTVDGVKFRCRAGMGRCQGGFCTSRIITIL
                     SERLGIPPEQVTKRGGGSWIMTPMNDPVVAAVAEVEV"
     gene            597..>1639
                     /locus_tag="PKH54_13435"
     CDS             597..>1639
                     /locus_tag="PKH54_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006103802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD00944.1"
                     /translation="MLGRMEGRYDVVIVGGGPAGLAAAIGAREGGAGRCLVIDREPEA
                     GGILLQCIHSGFGMHYFKQELTGPEYAERFLLDALEHDVDVVPDSYVCGIGLDSDGEK
                     RVRVLSDHYGLRTVSCRAVVLCMGCRERTRGAIRIPGTRPSGVLTAGLAQKFTNMMGY
                     LPGRRVAILGSGDIGLIMARRLVLEGCEVVGVFELMPYSNGLTRNVVQCLDDFGIPLN
                     LSTTVTEIMGQDRLSGIKVAPVGPGFKPDMSNERTIDCDTLLLSIGLIPENELSQSLG
                     INLDVTTKGPIVNSTLETSLPGVFACGNVLHVHDLVDFVTGEALRAGRHAGEYSRGIR
                     RAQDNIKLVPGDNV"
BASE COUNT          300 a          468 c          540 g          331 t
ORIGIN      
        1 tcatacgtca acaaactgtg tccgtcgatt catccgtccg atgccatcac ggcattcgcg
       61 ggcctgcgcg ccgtttccag cacaggggat ttcataatcg ggccgacatc ggtaaagggc
      121 ttcatcaacg cggccggcat acagtcgcct ggactgactt ccgcgccatc catcggcgag
      181 tacgtcgtcg gaatcctgga atccgagggg ctctcgatgt ccctcaagga tgatttcagg
      241 gccgagatcg acgggccgcc acgttttgcg aagcagcctg tcgatgttca ggaaaggatg
      301 gttgcatccg atcccgcgtt cgggaagatc gtctgtcgct gcgagattgt caccgaggcc
      361 gaaatccacg acgccatcga tcatggggcc aggacggttg acggcgtaaa gttcaggtgc
      421 cgggccggca tgggtcggtg ccagggcggt ttctgcacgt caaggatcat taccattctt
      481 tcggagcgtc ttggcatacc gccggaacag gtcaccaagc gtggcggcgg ttcctggatc
      541 atgacaccga tgaacgaccc ggttgtggcg gcggtcgcgg aagtggaggt atagccatgc
      601 tcggccggat ggaaggcagg tacgatgttg tcattgtcgg tggcgggccg gccggccttg
      661 ccgcggccat aggtgcccgt gagggcgggg ctggcaggtg cctggtcatc gatcgtgaac
      721 cggaggcagg cggaatcctg ctgcagtgca ttcactccgg gttcggaatg cactacttca
      781 agcaggaact gaccggcccg gagtatgccg agcgcttcct gctggatgcg cttgaacatg
      841 acgtcgatgt ggtgcccgat tcgtacgtct gtggaatcgg cctggacagc gacggggaaa
      901 agagggtcag ggttctttcg gatcattatg gccttcgaac cgtgtcatgc agggccgtcg
      961 tcctctgcat gggctgtcgc gagcgcaccc gcggcgcaat ccgcatcccg ggcacaaggc
     1021 cgtccggcgt ccttacggcc ggtctggcgc agaagttcac caacatgatg ggttaccttc
     1081 cgggccgtcg cgtggcgatc ctcggttccg gcgacatcgg gctgatcatg gccaggcgcc
     1141 tggttctgga agggtgcgag gtcgtcggcg tctttgaatt gatgccttat tcgaacgggc
     1201 ttaccaggaa cgtcgttcag tgcctggacg acttcggaat tcccctgaac ctttcgacca
     1261 cggtgaccga gatcatgggc caggacaggc tttcgggtat caaggtcgcg ccggtcggcc
     1321 ccgggttcaa gccagacatg tcaaacgagc gcacgattga ctgcgacacg ttgctgctgt
     1381 cgatcgggct gattcctgaa aacgagcttt cccagagcct tggcatcaac ctggatgtga
     1441 ccacgaaggg accgatagtc aactcgaccc ttgaaaccag cctgccgggg gtctttgcct
     1501 gcggcaacgt cctgcacgtt catgacctgg tcgatttcgt gaccggggag gcgctgcggg
     1561 ccggcaggca cgcgggcgag tattcgcgcg ggatcaggcg cgcccaggac aacatcaagc
     1621 tggtgccggg cgacaatgt
//
LOCUS       DAOPPA010000589         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_80807_length_1637_cov_16.431732, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000589 DAOPPA010000000
VERSION     DAOPPA010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80807_length_1637_cov_16.431732"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKH54_13440"
     CDS             complement(<1..1534)
                     /locus_tag="PKH54_13440"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOD00945.1"
                     /translation="MAGSGYIRFVTGDDAGIAAVDSEGLLRGALGDLRLHVFDSAGRL
                     VYRAGGAGGSIGVTVSADQTSSDGSLRIAVTLTNIGDATIRLSRAVFESFEGGESATI
                     QGDMSAWARYKMGFNTGAASGAAPLSVPDRRFFLRGVPYGLLPASVRNMLYYDGTEFS
                     KVPGRFESEWFSALHDLQNKRGILFGFCGVGRHFSRVRVDFPAGTASLTAFLDDSELA
                     PGASRELDPIIIVYGSDLNRLLADYAAAVAAQNGARVNPAAIWCSWYSGFYDRVRVTD
                     IRENMRRLSGIESPVRFIQLDDGYQAMIGDWLNTNSRFPEGVEALACEIRQAGFEPGI
                     WTAPFSVSPEGRVYAEHPDWVVRDRRGRPVKAGFIMGRFGPRPYYGLDTTIPEVRAHV
                     TGIYESLRRMGWRLFKIDFLTSAMVEGIRRDGSRTRAEAYRMGMQAVRDGVGDDGLIL
                     TGIGPFLANTGIVDIQRLSPDTCFGSPAWMTFEQKLTGDRMTPGVRNQTSTCIGRTFT
                     NDI"
BASE COUNT          345 a          533 c          471 g          288 t
ORIGIN      
        1 ggatgtcgtt ggtgaacgtc cggccgatgc aggtcgacgt ctggttcctg accccagggg
       61 tcatccggtc accagtcagc ttctgttcaa aggtcatcca tgccggcgac ccgaaacacg
      121 tgtccggact gagacgctgg atatcgacga taccggtgtt ggcaaggaac ggaccgattc
      181 cggtcaggat gagcccatcg tctccaacgc cgtcccgcac cgcctgcatt cccatgcggt
      241 aggcctcggc ccgggtacgg gaaccatccc ggcggatgcc ctcgaccatt gccgaggtca
      301 ggaagtctat cttgaacagc cgccatccca tccgccgcag cgattcgtat atcccggtca
      361 cgtgggcacg aacctcgggt atcgtggtat caagaccgta gtaaggcctc ggtccgaaac
      421 gtcccatgat gaaaccagcc ttgaccggcc ggccgcgacg atccctgacg acccagtccg
      481 gatgttcggc gtatactctg ccttctggag acaccgagaa tggggccgtc cagattccag
      541 gttcgaaacc ggcctgtcga atctcgcagg cgagcgcctc gacaccttcc gggaagcggg
      601 agttcgtgtt cagccagtcg ccaatcatcg cctggtagcc atcgtcaagc tggatgaaac
      661 gaaccggtga ctcgatcccg gaaagacgcc gcatgttttc gcggatatcc gtgactctga
      721 ctcgatcgta gaaccccgaa taccacgaac accatattgc ggccggattg acgcgagccc
      781 cgttctgcgc tgccacggcg gccgcgtaat ccgcgagcag cctgttcagg tccgaaccat
      841 agacaatgat gatcgggtcg agttcacgcg aagcgcctgg cgccagttct gagtcgtcaa
      901 gaaaggcagt cagcgaagcc gtgccggccg ggaaatcgac gcggacacga ctgaagtgcc
      961 ttccgacccc gcagaaaccg aaaaggattc cacgtttgtt ctgaagatcg tgcaaggccg
     1021 agaaccactc gctctcgaac ctgcccggca ccttggaaaa ctcggtgcca tcgtagtaaa
     1081 gcatgttcct gaccgatgcc ggaagcaggc cgtaagggac gccgcgcagg aagaagcggc
     1141 gatcaggaac cgacaacggc gccgcgcccg acgccgcacc ggtgttgaat cccatcttgt
     1201 agcgggccca cgcggacatg tcgccctgga ttgtggccga ttcgccacct tcgaaggact
     1261 cgaaaaccgc ccgggaaaga cggatggtcg cgtcgccgat gttcgtcagc gtcacggcga
     1321 ttcgcagcga accgtcactg gaggtttgat cagccgaaac cgttacgcca atcgatccac
     1381 cggcaccccc ggcccggtac accaagcggc ccgcactgtc gaacacatga agacgcaggt
     1441 cgccaagcgc acccctgagc agtccctcgg agtcgacggc cgcgatgccc gcgtcgtcac
     1501 ctgtcacaaa ccttatgtat ccggatcctg ccatagtccc tacatccggt ccgctcagaa
     1561 gcggtactgg aagtcaaacg caaactcgtt gttcgcgccg tgccccttcg cattcgccgg
     1621 cggcgtgcct atcgaat
//
LOCUS       DAOPPA010000590         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_80808_length_1637_cov_15.837547, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000590 DAOPPA010000000
VERSION     DAOPPA010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80808_length_1637_cov_15.837547"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKH54_13445"
     CDS             complement(<1..1534)
                     /locus_tag="PKH54_13445"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOD00946.1"
                     /translation="MAGSGYIRFVTGDDAGIASVDSEGLLRGALGDLRLHVFDSAGRV
                     VYRAGDAGGSIGVTVSTDRTASDGSLRIAVTLTNIGDATIRLSRAVFESFEGGESATI
                     QGDMSAWARYKMGFNTGAASGAAPLSVPDRRFFLRGVPYGLLPASVRNMLYYDGTEFS
                     KVPGRFESEWFSALHDLQNKRGILFGFCGVGRHFSRVRVDFPAGTASLTAFLDDSELA
                     PGASRELDPIIIVYGSDLNRLLADYAAAVAAQNGARVNPAAIWCSWYSGFYDRVRVTD
                     IRENMRRLSGIESPVRFIQLDDGYQAMIGDWLNTNSRFPEGVEALACEIRQAGFEPGI
                     WTAPFSVSPEGKVYAEHPDWVVRDRRGRPVKAGFIMGRFGPRPYYGLDTTIPEVRAHV
                     TGIYQSLRRMGWRLFKIDFLTSAMVDGVRRDGSRTRAEAYRMGMQAVRDGVGDDGLIL
                     TGIGPFLANTGIIDIQRLSPDTCFGSPAWMTFEQKLTGDRMTPGVRNQTSTCIGRTFT
                     NDI"
BASE COUNT          326 a          538 c          495 g          278 t
ORIGIN      
        1 ggatgtcgtt ggtgaacgtc cggccgatgc aggtcgacgt ctggttcctg acccccgggg
       61 tcatccggtc gccagtcagc ttctgttcaa aggtcatcca cgccggcgag ccgaaacagg
      121 tgtccgggct gaggcgctgg atatcgatga tgcccgtgtt ggcaaggaac ggcccgattc
      181 ccgtgaggat gagcccgtcg tccccgacgc cgtcccgcac cgcctgcatt cccatgcggt
      241 aggcctcggc ccgcgtacgg gaaccatccc ggcggacgcc gtcgaccatt gccgaggtca
      301 ggaagtcgat cttgaacagc cgccatccca tgcgccgcag cgattggtat atcccggtca
      361 cgtgggcgcg aacctcgggg atggtcgtgt caaggccgta gtagggccgg ggaccgaagc
      421 gtcccatgat gaaaccggcc ttgaccggcc gaccgcgacg atccctgacg acccagtccg
      481 ggtgttcggc gtagaccttg ccttccggcg aaaccgagaa gggcgccgtc cagattccag
      541 gttcgaaacc ggcctgtcga atctcgcacg cgagcgcctc gacaccttcc gggaagcggg
      601 agttcgtgtt cagccagtcg ccaatcatcg cctggtagcc atcgtcaagc tggatgaaac
      661 gaaccggtga ctcgatcccg gaaagacgcc gcatgttttc gcggatatcc gtgactctga
      721 ctcgatcgta gaaccccgaa taccacgaac accatattgc ggccggattg acgcgagccc
      781 cgttctgcgc tgccacggcg gccgcgtaat ccgcgagcag cctgttcagg tccgaaccat
      841 agacaatgat gatcgggtcg agttcacgcg aagcgcctgg cgccagttct gagtcgtcaa
      901 gaaaggcagt cagcgaagcc gtgccggccg ggaaatcgac gcggacacga ctgaagtgcc
      961 ttccgacccc gcagaaaccg aaaaggattc cacgtttgtt ctgaagatcg tgcaaggccg
     1021 agaaccactc gctctcgaac ctgcccggca ccttggaaaa ctcggtgcca tcgtagtaaa
     1081 gcatgttcct gaccgatgcc ggaagcaggc cgtaagggac gccgcgcagg aagaagcggc
     1141 gatcaggaac cgacaacggc gccgcgcccg acgccgcacc ggtgttgaat cccatcttgt
     1201 agcgggccca cgcggacatg tcgccctgga ttgtggccga ttcgccacct tcgaaggact
     1261 cgaaaaccgc ccgggaaaga cggatggtcg cgtcgccgat gttcgtcagc gtcacggcga
     1321 ttcgcagcga accgtcactg gcggttcggt cagtcgaaac cgttacgccg atcgacccgc
     1381 cggcatcacc ggcccggtac accacgcggc ccgcgctgtc gaacacatga agacgcaggt
     1441 cgccaagcgc gcccctgagc agtccctcgg agtcgacgga cgcgatgccc gcgtcgtcgc
     1501 ctgtcacaaa ccttatgtat ccggatcctg ccataatccc tacatccggc ccgctcagaa
     1561 ccggtactgg aagtcaaacg cgaactcgtt gttcgcgccg tgccccttcg cattcgccgg
     1621 cggcgtgcct atcgaat
//
LOCUS       DAOPPA010000591         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_80836_length_1637_cov_2.840708, whole genome shotgun sequence.
ACCESSION   DAOPPA010000591 DAOPPA010000000
VERSION     DAOPPA010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80836_length_1637_cov_2.840708"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PKH54_13450"
     CDS             <1..626
                     /locus_tag="PKH54_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00947.1"
                     /translation="LDWIGENRSGSIVDEYCAIFFDKSKYELIETKTLALSETPEVIG
                     TKFDEEDQLYPRIVTWGHFRRIADRFEFDVFSTHWDHTGEEGIREKMAVVTLQQMASL
                     ANGRPVLLAGDFNCGFQSLPWNVMTGAAEYQGVTGSMIDTWVELGLPEEGTYHAFTGV
                     TSGNRIDWVMHNGGFKAVSAEILKSSYDGIYPSDHFPIGAEFRITKP"
     gene            695..>1637
                     /locus_tag="PKH54_13455"
     CDS             695..>1637
                     /locus_tag="PKH54_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00948.1"
                     /translation="MNTRIVPPLAALLVALTPCPSHATESTGQDGPPVLVWGVTATED
                     VPRESVDMLSFTLVDEFDAVTGMATTDDQLVDAGIATVADSDEWSEDPLLFLAAVARQ
                     TRSAEAVTGELNDVGGALVLTLQRIDSGTGLVVRQVTGQTRDDVQILVDSIPEMIARL
                     YDIEYQTAGTVAESGSRNGYSYVVATFGNPFEWIKPLARIGRLPLWGHGETRHNQPRQ
                     EISGIRTGAGESVHARPRSMLRPERRMPPPRHVMPDRPDPRRERPVVRPERNMPPPRH
                     VMPDRPDPRRERPVVRPERNMPPPRHVMPDRPDPRRER"
BASE COUNT          349 a          487 c          496 g          305 t
ORIGIN      
        1 gcctggactg gatcggtgaa aaccgaagtg gaagcatcgt ggatgagtac tgcgccatct
       61 tctttgacaa gtcgaaatat gagcttattg aaacgaagac gctggcgctg agcgaaaccc
      121 cggaagtcat cggcacgaag ttcgacgagg aagaccagtt gtatccgcgc atcgtgacct
      181 ggggccactt ccggaggatt gcggatcgct tcgaattcga cgtcttttcg acccactggg
      241 atcacactgg cgaagaaggc atccgggaaa agatggccgt tgtcacgctg caacagatgg
      301 cttccctggc caacggccgt ccggtgctcc tggcgggcga cttcaactgc gggttccagt
      361 cgcttccgtg gaacgtcatg acgggcgccg cggaatacca gggagtgacc ggcagcatga
      421 tcgatacctg ggttgaactc gggttgcccg aggaaggtac gtaccacgct tttactggcg
      481 tcacctccgg aaaccggatc gactgggtca tgcacaacgg cggcttcaag gccgtgtcgg
      541 ccgaaatcct gaagtccagc tatgacggca tatatccgtc agatcatttc ccgatcggtg
      601 ccgaattcag gatcacgaag ccttgatctt tttccccccg caccgatcca tgccgctata
      661 atgccggtat cgaaccgatg atccggaaga tgaaatgaat acacgcattg ttccgcctct
      721 tgcggccctg ttggtcgcgc tgaccccatg tccatcacat gcgaccgaat cgaccgggca
      781 ggacggcccg ccggtactgg tctggggcgt gacggccacc gaggatgtgc cgagggaatc
      841 ggtcgatatg ctttcattca ccctggtcga tgaattcgac gccgtcacag gcatggcgac
      901 gacggacgat caactggtgg atgccgggat cgcgaccgtg gcggattccg atgaatggtc
      961 ggaagatccg ttgctgttcc tggctgccgt cgcacgccag accagatccg ccgaggccgt
     1021 cacgggcgaa ttgaacgatg tcgggggcgc actcgtctta acgcttcagc gaatcgattc
     1081 cggcaccggg ctggttgtca ggcaggtaac cggtcagacg cgtgacgatg tccagatcct
     1141 tgtggactcc attcccgaga tgattgcgcg tctttacgac atcgagtacc agaccgccgg
     1201 aaccgttgcc gaatcgggca gcaggaacgg atacagctat gtagttgcga ctttcggaaa
     1261 tccctttgaa tggatcaagc cgctcgccag gattggcagg ctgccgttat ggggccacgg
     1321 agaaaccaga cacaatcagc cccgccagga aatttcgggg ataaggaccg gtgccggcga
     1381 aagtgtccat gccaggccgc gatcaatgct ccggcccgaa cgacggatgc ctccgcccag
     1441 gcacgtcatg ccggacaggc cggatccacg gcgcgaacgt cctgtcgtcc gaccggaacg
     1501 aaacatgcct ccgcccaggc acgtcatgcc ggacaggccg gatccacggc gcgaacgtcc
     1561 tgtcgtccga ccggaacgaa acatgcctcc gcccaggcac gtcatgccgg acaggccgga
     1621 tccacggcgc gaacgtc
//
LOCUS       DAOPPA010000592         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_81171_length_1632_cov_2.934686, whole genome shotgun sequence.
ACCESSION   DAOPPA010000592 DAOPPA010000000
VERSION     DAOPPA010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81171_length_1632_cov_2.934686"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1091
                     /gene="thiD"
                     /locus_tag="PKH54_13460"
     CDS             267..1091
                     /gene="thiD"
                     /locus_tag="PKH54_13460"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOD00949.1"
                     /translation="MMGRKFWVAVPRALTIAGSDCSGGAGLQADLKVFFRFGVFGQSA
                     VTAVVAENTVGVQGVAAVRPDFVALQIDSCLSDVGADVVKTGMLVDSDIVQAVAERLR
                     FHDVRRLVIDPVMCAKNGDRLIAVEAVHTLVNSLFPLATVVTPNVPEAEALWGRKIQS
                     VMDFRTAAVAIRAMGPRVVVMKGGHSPVNPEGESVDYVLSGDDWVPISGPRGSDRNTH
                     GTGCTFSAAIAARMARGDDPVTAVRVAKQYITDAIATAPDLGHGHGPVNHWAELNR"
     gene            1301..>1632
                     /locus_tag="PKH54_13465"
     CDS             1301..>1632
                     /locus_tag="PKH54_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00950.1"
                     /translation="MSFKAPISLIVIPAVVCCGFGCIKPEPARDIMSDDIIVGDSLID
                     SSDPDSVDWDTLLDGVVDVKPEDICGLVDADPVHVEGQPCEDEGESYCIGDGAYSELI
                     PDFSRQCIR"
BASE COUNT          325 a          435 c          503 g          369 t
ORIGIN      
        1 gcaatgaatg acattcagac atctggggtt aattgaaact cagttctgaa tgtgtggcca
       61 aattggttga ctttaccagt cggttaatta tgaaaatcgg tatcggcatc ccaaaaatga
      121 acttcgcatc accgataaag tcggttaaaa ctaacacttt caatctgacg aaacaagatt
      181 tcgtggccgg attgattacg gacgaattga ttttgtcgtc tgattgtgcg acatcgacgc
      241 tgggttcgaa tactgatgga tttttgatga tgggcagaaa gttctgggtg gccgttccca
      301 gggcgctgac gatagccggg tcggattgca gcggcggggc cggtctgcag gcggacctga
      361 aggtcttttt caggttcggc gtcttcgggc agtcggccgt caccgccgtc gtggccgaaa
      421 acacggtggg tgtccagggc gttgccgcgg tgcgccccga cttcgtggcc ctgcagatcg
      481 acagttgcct gtccgacgtg ggcgccgacg tggtcaagac cgggatgctg gtcgattcgg
      541 atattgtcca ggcggttgcc gaacggctgc gcttccacga cgtgcggcgg ctggtcatcg
      601 acccggtgat gtgcgccaag aacggtgacc gcctgatagc ggttgaagcg gtccataccc
      661 tggtcaatag cctgtttccg ctggcgaccg tcgtgacccc gaatgtcccg gaggccgagg
      721 ccctgtgggg ccggaaaatc cagtccgtga tggacttccg gaccgccgcc gtggccatcc
      781 gcgccatggg cccgcgcgtc gttgtcatga agggcgggca cagccccgtc aatccggaag
      841 gcgaatcggt cgattacgtg ctttcgggcg acgactgggt tccgatttcc ggaccgcgcg
      901 gcagcgaccg caacacccac ggcaccggat gcaccttctc ggccgccatc gccgcccgca
      961 tggcccgcgg cgacgatccg gtcacggccg tccgcgtcgc caagcagtac atcaccgacg
     1021 ccatcgccac cgcccccgac ctgggccacg gccacggccc cgtcaatcac tgggcggagt
     1081 tgaatcggta agtccgcagc aggctgttga tttcttgcgc tggattgttc ctgggcggag
     1141 tgtgcagata ccgctggcgt cacgatatcc cgtgagggaa ggtgggcgac ggacctaagg
     1201 catctgatcc agaagcacct tgattttaat agtcaggtta tggatgatga taaatgatgt
     1261 aaatgaccgt caaataccag aatgccccca ggggaaatat atgtcgttca aagctccgat
     1321 ttcattgatt gtcattcctg ctgtcgtctg ttgcggtttc ggctgtatca agccagagcc
     1381 cgcaagggat attatgtctg atgacattat tgttggcgat tcgttaattg actcaagcga
     1441 tcccgactca gttgactggg acacattgct tgacggtgta gtcgatgtga agcccgagga
     1501 catctgtggg ctggttgacg ctgatccagt gcatgttgag gggcagcctt gtgaagatga
     1561 aggcgagtcg tactgcattg gagatggggc ttacagtgag ctgattcccg acttttcccg
     1621 gcagtgcata cg
//
LOCUS       DAOPPA010000593         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_81403_length_1629_cov_3.050826, whole genome shotgun sequence.
ACCESSION   DAOPPA010000593 DAOPPA010000000
VERSION     DAOPPA010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81403_length_1629_cov_3.050826"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1225
                     /locus_tag="PKH54_13470"
     CDS             26..1225
                     /locus_tag="PKH54_13470"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD00951.1"
                     /translation="MRKVEGDSLSVEGLRTGRYTVKAESSLPCFEPMEKVIWSPFGLG
                     LSVHDFKLKRVTGTVSVGTLPAGATLTVKNPVEEKSCTTPCRLQNLLAIESDITVSLG
                     EMTPVTMRETIPANGVLLPGFISFKGEVEVVTIPEGAVATLGGRESITTPGRFRGLLP
                     MGQTLKVEKPGYITQYSDQSVVDGQLTKVRVELVPRYGTVVVNGRLPDGSACKGNVTI
                     DGIQVGNTGNSFQTLAKETTIGVECEKGAGSVNGFVVKENEKKFVSVKVSPLEWAQSR
                     GLLKVVNRTAWQYGGMAICQDWFSADIYCQDLMHAHYLDWRLPTRREMIELEDSGEFV
                     NLLIPGEYYWSEAASDWEAVAYKVGGQGKGRREVVEKGSVQLCNIVCARTLGADEGIP
                     PTPPVGN"
     gene            1341..>1629
                     /locus_tag="PKH54_13475"
     CDS             1341..>1629
                     /locus_tag="PKH54_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00952.1"
                     /translation="MKQIIASAFTVLMIAGCGAPLARVDAGNRQSSVRGDSDGKASAE
                     PADTELDENAVKLDACVRKGPGGRNVISPECIRQDISVLPGMKAKDLGTVGM"
BASE COUNT          346 a          387 c          537 g          359 t
ORIGIN      
        1 tgccacagga tgctgataag cgctgatgcg gaaggttgaa ggtgattcgc tgtcggtcga
       61 gggcctgaga accggccgtt acaccgtgaa ggccgaatcg tcgctgccct gcttcgagcc
      121 gatggaaaag gtgatctggt cgccattcgg tctgggcctt tcggttcatg atttcaaatt
      181 gaagcgcgtc accggaaccg tttcggtcgg cacgcttccc gccggtgcga cattgaccgt
      241 taagaacccg gtcgaggaga agtcgtgtac gacgccatgc cggcttcaaa atcttctggc
      301 catcgaatcc gacatcacgg tatccctggg ggaaatgact ccagtaacaa tgcgtgagac
      361 gattccagcg aacggggtgc tgttgcctgg tttcatttcc ttcaagggtg aagtcgaagt
      421 ggtgacgatc ccggagggtg ccgtcgcgac ccttggtggc agggaatcca taaccacgcc
      481 aggcaggttc aggggccttc tgccaatggg gcagacgctg aaggtcgaaa agcctggtta
      541 catcacgcag tattcggatc agtctgttgt cgatggtcag ttgacgaagg ttcgggtcga
      601 acttgttcca cgatatggaa cggttgtcgt caatggacgt cttcccgacg gctcggcgtg
      661 caagggaaat gtaacgattg acgggattca ggtcggaaat accggaaatt cgttccagac
      721 tcttgcgaag gaaacgacta ttggggtcga gtgcgaaaag ggtgctggat cggttaacgg
      781 cttcgtggtc aaggagaacg agaagaagtt tgtcagcgtc aaggtgtccc cgcttgagtg
      841 ggcgcagtca aggggcctgt tgaaggttgt caaccgcacg gcctggcagt acggcgggat
      901 ggcaatctgc caggactggt tcagcgccga catttattgc caggatttga tgcatgccca
      961 ctatctggat tggcgccttc ccaccaggcg ggaaatgatt gagcttgaag attcgggtga
     1021 attcgtgaat ctgttgatcc ccggcgaata ctactggtca gaggcggcga gcgactggga
     1081 agccgttgcg tacaaggtcg gtggccaggg caaggggcgt cgcgaagttg tggagaaggg
     1141 ttcggttcaa ctctgcaata ttgtatgtgc caggacactt ggggccgatg aaggaattcc
     1201 gccgacgccg cctgtcggca attgaccggt ccgtcttgac ttgaatggac tgttgcgggt
     1261 aaatggcata tcgcgtcgaa tcgagaaatc ttgaaggggt ttcgggccgg cccgtttgac
     1321 ccggcatttc tggggatttg atgaaacaaa taattgcttc agcgttcacg gtcttgatga
     1381 ttgccggttg cggggcgcct cttgcccggg tcgatgccgg gaatcggcag tcttcggtgc
     1441 gtggcgattc ggatggcaag gcgtccgcgg agccggccga cacggaactg gacgaaaatg
     1501 cggtgaagct tgatgcatgc gtgcgcaagg gcccgggcgg acgaaatgtc atcagtccgg
     1561 aatgcatccg tcaggacata tccgtactgc cgggaatgaa ggcaaaggat cttggaaccg
     1621 tcggcatgg
//
LOCUS       DAOPPA010000594         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_81675_length_1625_cov_3.594904, whole genome shotgun sequence.
ACCESSION   DAOPPA010000594 DAOPPA010000000
VERSION     DAOPPA010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81675_length_1625_cov_3.594904"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKH54_13480"
     CDS             <1..283
                     /locus_tag="PKH54_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00953.1"
                     /translation="FGATKLYCGVQDYYSGSYYGTTTLEKAMYPVSNVPTVVKATELA
                     AATTTGQVGCTLNYWGTMDIQPRFSLGRSDAIDFSGFTGAEYASAGPLP"
     gene            complement(305..>1625)
                     /locus_tag="PKH54_13485"
     CDS             complement(305..>1625)
                     /locus_tag="PKH54_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOD00954.1"
                     /translation="GGTPEELETFASALYRVFLMPTLVTDADGFYPAMDKTAKKVEWG
                     TYYSDFSLWDTYRTEHPLLALVYPEHQRDFVMSMIQMARDGGYLPLWPFVNGETNIMV
                     GAPAEIVVADSWLKGIDFPHEEGFDLALRTALGPADEDSQFFGREGIEEYLENGWVAA
                     DLQGGSVSKTLEYAVADNALCLMARGLARTDAEGTLCANSQTYRNLWHPDHKFFLGRA
                     ADGTFPRAATFDPAAIAGDVLAESNGDYTEGNPWHYLWFVPHDPAGLIELNGGDETFV
                     SRLSAFFADSAAEEDGIPDDIESKGVSRLYYWHGNEPGLHTAYLFSSAGRQDLACLTV
                     RDLMTTNYGPEPDDLPGNDDCGALSAWYVFSAMGLYPNAGTNDYWLGCPVFDRMRVRL
                     PGGILEIVDGTGDMAATGARLDGEPVGGGTITWESLKDGGVLSFGAD"
BASE COUNT          293 a          497 c          519 g          316 t
ORIGIN      
        1 cttcggggcg acaaagctgt attgcggggt gcaggattac tattcgggtt cttactacgg
       61 caccacgacc ctggaaaagg cgatgtaccc ggtttcgaac gttcccacgg ttgtcaaggc
      121 gaccgagctt gcggccgcca cgacgaccgg ccaggttggt tgcacgctaa actactgggg
      181 tacgatggat atccagccgc gcttctcgct gggcaggtcg gacgcgatcg acttttccgg
      241 cttcaccggc gccgaatatg cgagcgcggg cccgcttccg taaccagatg acataaattg
      301 gttttcagtc ggcgccgaaa gacagcacac cgccgtcctt caggctttcc catgtgattg
      361 ttccgccgcc cacaggttcg ccgtcaagtc tggcgcccgt tgcggccatg tctccggttc
      421 cgtcaacgat ttcaaggatt ccgcccggaa ggcggacgcg catgcggtca aacacgggac
      481 acccgagcca gtagtcgttc gtcccggcgt tcgggtacag gcccatcgcg gaaaagacgt
      541 accaggcgga cagggcgccg cagtcgtcgt tgccgggaag gtcgtccggc tcggggccgt
      601 agttcgtcgt catcaggtcg cggacggtca ggcaggcgag atcctggcgc ccggccgacg
      661 agaataggta ggcggtgtgc aacccgggct cgtttccgtg ccagtagtaa aggcgggaaa
      721 cgcccttgct ttcgatgtcg tccgggatgc cgtcctcttc cgcggcggag tcggcgaaga
      781 aggccgaaag ccgcgaaacg aatgtttcgt cgccaccgtt aagttcgatc agcccggccg
      841 ggtcgtgggg cacgaaccac aggtaatgcc aggggtttcc ttccgtgtag tcaccgttgc
      901 tttcagcaag gacgtccccg gcgatcgcgg ccgggtcgaa cgtggcggcg cgggggaatg
      961 ttccatcggc tgcgcggccc aggaagaact tgtgatcggg gtgccacagg ttacggtagg
     1021 tctggctgtt tgcgcacagc gtgccttcgg cgtcggtacg ggccagcccc ctggccatca
     1081 ggcaaagggc gttgtcggca accgcgtact caagcgtctt tgaaaccgat ccgccctgaa
     1141 gatccgcggc gacccacccg ttttcaaggt attcctcgat gccttcgcgg ccgaagaact
     1201 gggaatcctc gtcggccggg cccagtgcgg tgcgcagggc aaggtcgaag ccttcttcat
     1261 gcgggaaatc gatacccttc agccaactgt ccgcgaccac gatttccgcc ggcgccccga
     1321 ccatgatgtt ggtttcgccg ttgacaaacg gccacagagg caggtacccg ccgtcacggg
     1381 ccatctggat catcgacatc acgaagtcgc gctggtgttc gggatagaca agggccagca
     1441 gcgggtgttc ggtccggtac gtgtcccaca gcgagaagtc gctgtaatac gtgccccatt
     1501 cgaccttctt cgcggtcttg tccatggcgg gatagaagcc gtccgcgtcg gtcaccagcg
     1561 tcggcatcag gaatacccgg taaagcgcgc tggcaaacgt ttcaagttct tccggggttc
     1621 cgcct
//
LOCUS       DAOPPA010000595         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_81741_length_1624_cov_3.927342, whole genome shotgun sequence.
ACCESSION   DAOPPA010000595 DAOPPA010000000
VERSION     DAOPPA010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81741_length_1624_cov_3.927342"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PKH54_13490"
     CDS             <1..1239
                     /locus_tag="PKH54_13490"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00955.1"
                     /translation="LPIVSNATETPSTVSGPSGMKMTGPYNWVPPKYWYVSVPEDPAA
                     LADEIDWPWLYGGAYGFNSESSPGPAVPPLDSMLKMLPEEDIWPVKDPFLFHSGGTST
                     ARERMKVFNDALAARLGTPTDAADYTFKAQIMAYESHRAMFEAQGRNKYFATGHIQWM
                     LNNAWPGTIWNLYDYYLRPAGSYFGSKKALAPLHVQYSYDDESVWVVNSTLTEFKDLT
                     ISATIWNLDGKIAYSSTSDPFDVPPDGNQEVMSRVIPASAPLTDLDQVHFIDLRAFDS
                     AGNEIDRNIYWVSTVPDTFDMVYRDYELPQVEVADMTALGALPEAVLSIPDFTTRTSG
                     DRRIITQKLKNIGESIAFFIETLLVDLDSGEPILPAIYSDNYVTLMPGEEIEITVSVA
                     AGPTAALRLGTRVSGWNVRP"
     gene            1329..>1624
                     /locus_tag="PKH54_13495"
     CDS             1329..>1624
                     /locus_tag="PKH54_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00956.1"
                     /translation="MDRASLFSIRGLLVATAAFHLAACGADQETPDVLQDVPPDIATD
                     TPAADGAQQDGADDAGAGFNQVATPWKPGPAAPESDLFDDMGLVDSTGGFRKFNG"
BASE COUNT          345 a          511 c          468 g          300 t
ORIGIN      
        1 ctgccgatcg tgtcgaacgc caccgagacg ccgtcaacgg tttccggccc cagcggaatg
       61 aagatgaccg gaccttataa ctgggttcca ccgaagtact ggtatgtatc ggtgcccgag
      121 gatccggccg cgctggccga cgaaatcgac tggccatggc tttacggcgg ggcatacgga
      181 ttcaacagcg agtcaagccc gggaccggcc gttccgcctc tggacagcat gttgaagatg
      241 ctgccggaag aagacatctg gccggtcaag gatccattcc tgtttcattc cggcggcact
      301 tcgaccgcgc gcgaacgcat gaaggtcttc aacgacgccc ttgccgccag gctgggcacc
      361 ccgaccgatg cggccgacta cacgttcaag gcccagatca tggcttacga gtcccatcgc
      421 gccatgttcg aggcccaggg acgcaacaag tacttcgcaa ccggccacat ccagtggatg
      481 cttaacaacg cgtggccggg aaccatctgg aacctttacg attattacct gcggccggcc
      541 ggcagctatt tcggctccaa gaaggccttg gccccgttgc acgtgcagta ctcgtacgac
      601 gacgagtcgg tctgggtcgt caactccacg ctgactgaat tcaaggattt aacgatatcc
      661 gcgaccatct ggaacctgga cggaaaaata gcttattcat cgacatcgga tcccttcgac
      721 gtgccgcccg acgggaacca ggaagtaatg tcccgggtga ttccggcgtc cgcacctttg
      781 actgaccttg accaggttca cttcatcgat cttcgggcgt tcgactcggc gggaaacgaa
      841 atcgaccgca acatctactg ggtttcaacg gttcccgaca ccttcgacat ggtctatcgc
      901 gattacgaac tgccccaggt tgaagtggcc gacatgaccg ccctgggcgc ccttcccgag
      961 gccgtccttt cgattccgga ctttacgacg cggacgtccg gcgacaggcg gatcatcacg
     1021 cagaaattga aaaacattgg ggaatcaatc gcattcttca tcgaaacact gcttgtcgat
     1081 ctcgactccg gcgaaccgat cctgccggca atctacagcg acaactacgt aacgctcatg
     1141 ccgggcgagg aaatcgagat cacggtttcg gttgcggccg ggccgactgc cgcgctcaga
     1201 ctcgggacac gcgtgtccgg atggaacgtg cggccctgac gcacgggccc ggacgacggt
     1261 catccgctct gaattgacgt gccggaaagg caaaactatc atcacgccaa cccatcgtgg
     1321 atttcatcat ggacagagcc agccttttct cgatccgggg actgcttgtc gcgaccgcgg
     1381 cgtttcacct ggccgcgtgc ggggccgatc aggagacacc cgatgtctta caggacgtgc
     1441 cgccggacat cgccacggac acaccggcgg cagacggcgc ccagcaagat ggggcagacg
     1501 acgccggggc aggcttcaac caggtcgcca ccccctggaa gcccggaccg gctgcacccg
     1561 aaagcgacct gttcgacgac atgggcctgg tcgattcaac cggtgggttc aggaagttca
     1621 atgg
//
LOCUS       DAOPPA010000596         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_82415_length_1615_cov_2.973718, whole genome shotgun sequence.
ACCESSION   DAOPPA010000596 DAOPPA010000000
VERSION     DAOPPA010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82415_length_1615_cov_2.973718"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1327
                     /locus_tag="PKH54_13500"
     CDS             149..1327
                     /locus_tag="PKH54_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOD00957.1"
                     /translation="MKRKQFADGVFWMGAVDWDRRVFDSLIPTPEGTSYNSWLITTPE
                     KTVLFDTVDPAKTDVLMSQLADIERIDYIVTHHAEQDHSGSLPVVMKEFPEAVVLCTP
                     KCRSILTRLMPLDANRIRVVEDGEILDIGGGRKLQFMHMPWVHWPETMVTWCPTDGIL
                     FSCDLFGSHLATSQLVVPWEVVYDPAKRYYAEIMMPFRQKIAGYLERLDALPIKMICP
                     SHGPMHADPAPIMNSYREWVGGKPANKVLIPFISMHGSTERMVSLLVSELAALDVIVK
                     PFNMIETDLGQLAESAIDAATIILASPTVLGGAHPFVLSAAAVVGLLKPKTMYLGVMG
                     SYSWGGRMPDQLAAAVSTLKAEVLEPLLVEGYPDGEDAARIRELARKIHDLHAAAGLK
                     "
BASE COUNT          332 a          478 c          462 g          343 t
ORIGIN      
        1 ctggagcgca acggcggcct gcccaacccg gacgacaaga taaatgaggg cgatatcctt
       61 gtggtcatcg ggagtcccga aatgatatcg gcatttctcg aattgacctg atattatcgg
      121 ctggtttgat tttctggagc ataacggcat gaaaagaaag cagttcgctg atggcgtttt
      181 ctggatgggc gcggttgact gggatcgccg cgtcttcgac tcgctgattc cgacaccgga
      241 aggaaccagc tataactcat ggctgataac gacgccggaa aagacggtgc ttttcgacac
      301 ggttgacccg gccaagaccg acgtcctgat gtcccaactg gctgatatcg aaaggataga
      361 ctatatcgtc acgcatcacg ccgagcagga tcattcgggc tcactgccgg tcgtcatgaa
      421 ggaatttccg gaagcggtgg ttctctgcac gcccaagtgc cgatccatcc tgaccaggtt
      481 gatgcccctt gacgccaatc ggataagagt cgtcgaggac ggtgagatcc tggacatcgg
      541 tggcggcagg aagctgcagt tcatgcacat gccctgggtt cactggccgg agaccatggt
      601 gacctggtgc ccgaccgacg gcatcctgtt ttcatgcgac cttttcggtt cacacctggc
      661 gacgtcgcag ctcgttgttc cgtgggaggt cgtctatgac ccggccaaaa ggtactacgc
      721 cgaaatcatg atgccgttca ggcagaagat tgccggatac cttgaacgcc tggatgcctt
      781 gccaatcaag atgatctgcc cttcccacgg tccgatgcac gcagatccgg ctcccatcat
      841 gaattcgtat cgggaatggg tcggtgggaa gccggccaac aaggttctga tcccgttcat
      901 ttccatgcat ggaagcaccg aacgcatggt ttccctgctg gtttccgaac tggcggcgct
      961 tgatgtcatc gtcaagccgt tcaacatgat tgaaacagat cttggccaac tggccgaatc
     1021 cgcaatcgac gcggcaacaa tcatactggc ttcgccgacg gtccttggcg gggcgcaccc
     1081 cttcgtgctt tccgccgccg cggtggtcgg actgcttaaa ccgaagacca tgtatcttgg
     1141 cgtcatggga tcatattcgt ggggcggacg catgcctgat caactcgccg cggcggtatc
     1201 cacacttaag gccgaagtcc tggaacccct gcttgtcgaa ggctatccgg acggcgagga
     1261 tgccgccagg attcgcgaac tcgcccgaaa gatccacgac ctgcacgccg ccgccggcct
     1321 gaagtaaccc ttcggcccgc cttgctggtt cgacagactc accacaggtc gctcacgtca
     1381 cgccccacag tccattgttc cacgctcgac gagtgacgca gtcgtgcctg ttgccggcca
     1441 tgaagtatcc ggttcgagag gcctcaccgg acactcatgg tgcggcccga tccgcccccg
     1501 atcggcaggg gcgaatgaga attcgcccgc ctgcctgaga attcgcccgc ctgcctgaga
     1561 attcgcccgt ctgcgtgaga catttttagc ggtggaaatg ctagatgtcg ccggc
//
LOCUS       DAOPPA010000597         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_82758_length_1610_cov_3.964630, whole genome shotgun sequence.
ACCESSION   DAOPPA010000597 DAOPPA010000000
VERSION     DAOPPA010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82758_length_1610_cov_3.964630"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1610)
                     /locus_tag="PKH54_13505"
     CDS             complement(<1..>1610)
                     /locus_tag="PKH54_13505"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD00958.1"
                     /translation="NNIVGNAVQGALVIRDVGLTVSTDSDWYGTKASGTWTNPSNATI
                     LSVQMQYSESDSSSNYVSGAVRKDSSSGTEMASASSATQRWYDVSSYGAKTIVATVSD
                     YYSGSYYGTTTVHKAVSLESGVVREVSAITQSGVIDMRHNYFGVFPNVLDRVSLGSTT
                     SANLHGFVNQEFDSGWSKGNCFGGETISTETTWIGTIYVTGDLTFAGTTLNVGAGTQV
                     LFAPVDQDGNGIGDYDLNLSASTFNVNGTSGDPVRFGTLGDVAQGFQEVRVFGAGTST
                     IQNAIFENGRVGLTVETGTHSIAASTFRNNGGQGLWLKSNSGSYSVTVSGGLSRDNGV
                     GVLVESFRNLSIDRMTVEYNTGNGIQFGSSTTSVGLTNCTVRNNGGDGVYLNNSTVGI
                     DHCSIQYNGAFGVRYRGSSAGTLSYSNIKFNDMAGVLLLSSSSSPNPQITSNNIYGNS
                     LDSGIDVASPALSVSTDSDYYGTKWSSAWSTPGGQKIYWIRASYSESDSSSNYVTGQI
                     TAGSQGGTAVFSASSASSASFLDIESFGA"
BASE COUNT          336 a          499 c          433 g          342 t
ORIGIN      
        1 gccccgaagg actcgatatc caggaaactg gccgaagaag ccgacgacgc cgaaaacacc
       61 gccgttccgc cctgtgaacc ggccgtgatc tgtcccgtca catagttgct gctgctgtcg
      121 gattccgaat atgaggccct gatccagtaa atcttctggc cacccggagt actccacgcg
      181 ctggaccatt tcgttccgta ataatcggaa tcggtgctca cgctcagggc cggcgatgcg
      241 acatcaattc cggaatccag cgaattccca tagatattgt tactggtgat ctgcggattc
      301 gggctcgacg acgacgaaag cagcaacacg ccggccatgt cgttgaactt tatgttggaa
      361 tatgacagcg ttccggccga ggagcccctg tacctgacac cgaaagcacc gttgtactga
      421 atgctgcagt gatcgatacc gaccgtcgaa ttattcaggt aaacgccgtc gcccccgtta
      481 ttgcgtaccg tacagttggt cagtccaacc gaagtcgtgc ttgagccgaa ctggatgccg
      541 ttgcccgtgt tgtactcgac ggtcatcctg tcgatcgaca ggttcctgaa cgactcgaca
      601 agcacgccga cgccattgtc cctggaaagg ccaccggaaa ccgtcaccga gtacgagccg
      661 ctgttggact tcagccacaa gccctgaccg ccgttattcc tgaacgtgct tgcggcaatc
      721 gagtgggttc cagtctcaac ggtcaagcca acgcgcccgt tttcgaatat cgcgttctga
      781 atcgtgcttg tcccggcccc gaagaccctg acctcctgga aaccctgcgc cacgtcgcca
      841 agcgttccga accggacggg gtcgcccgaa gtgccgttca cgttgaacgt tgaagcagac
      901 aggttaaggt cgtagtcccc aattccgttc ccatcctgat cgacaggagc gaaaagcacc
      961 tgggttccgg cccccacgtt aagggtcgtt cctgcaaacg tcagatcccc ggtgacatag
     1021 atggttccaa tccacgtcgt ttccgtggag atcgtctcgc cgccgaagca gttgcccttg
     1081 gaccacccgg aatcgaactc ctggttgacg aaaccgtgca agtttgccga agtcgtcgag
     1141 cccaggctga cgcgatcaag aacgttcggg aacaccccga agtagttgtg ccgcatgtcg
     1201 atcacgccgg attgggtaat ggccgacacc tcgcgaacca ccccggattc caggctgacc
     1261 gccttgtgca cagtcgtggt gccgtaatag gaaccgctgt agtaatccga aaccgttgcc
     1321 acgatggtct tcgcgccgta cgaagaaacg tcgtaccagc gctgggtcgc gctgctggct
     1381 gatgccatct cggttccgct cgatgaatcc ttcctgaccg caccgctgac gtaattgctg
     1441 ctgctgtcgg attcactgta ctgcatctgc acgctcagaa tcgttgcatt cgagggattg
     1501 gtccacgtac cgcttgcctt ggtgccgtac cagtcggaat cggtcgagac cgtcaaaccc
     1561 acgtccctga tgaccagcgc gccttgaacc gcgttcccga cgatgttgtt
//
LOCUS       DAOPPA010000598         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_83036_length_1606_cov_3.862025, whole genome shotgun sequence.
ACCESSION   DAOPPA010000598 DAOPPA010000000
VERSION     DAOPPA010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83036_length_1606_cov_3.862025"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKH54_13510"
     CDS             <1..539
                     /locus_tag="PKH54_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00959.1"
                     /translation="MGWVEPGSASAVTDTGLKVTLNTMNGFKLVRQYQLDTLEPVLAV
                     TTTLTNPGTATVSTRLRPHLEMDLGTLEDTTVEFVARDGTTVSRNMDGVIAGMRQGEH
                     FYDQKAPDGKWSFTGSKGLRLTYGFDENLVDYTWLYAYPARDGELEMEVFARAVTLAP
                     GESFTFMTWIEIEPAGSK"
     gene            710..>1606
                     /locus_tag="PKH54_13515"
     CDS             710..>1606
                     /locus_tag="PKH54_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00960.1"
                     /translation="MRNVLLALLSLSVVATFAGCEYPTVQDGVDGPALGTFNTSGCKL
                     DNASQRSLMDGNMIEAVVFETSVTIIHHDAQYQCNAEIGWELEVNGTTLILREVDKST
                     VVTRCMCPMDLSTTIENLAAGGKYTIQVWDEFQTRMFGEVTVDLGDCNEQCVTDEDCW
                     AYPDLPRLDCEGRWACIKGLCNYACEPYDPGCTSDYDCPEGFQCVFYSNGGGSAETSP
                     DGTMAQRMMAYECKSNEDCPEGMVCEMTDCACPPGADCDCYPYGYCTGGQVWPTEGYC
                     EPIVINNECRSDADCGSGYHCEF"
BASE COUNT          333 a          436 c          503 g          334 t
ORIGIN      
        1 tcatgggctg ggttgagccc ggttcggcgt ccgcggttac ggacaccggc ctgaaagtca
       61 cgttgaacac gatgaacggt ttcaagctgg tccgccagta tcaacttgac acgctggagc
      121 ctgtgcttgc ggtcacgacg accttgacca atccgggaac cgcaaccgtt tccacccgac
      181 ttcgtccgca cctggaaatg gatcttggaa cgcttgagga cacgaccgtc gagtttgtcg
      241 cgcgggacgg aacgacggtt tcccggaaca tggacggcgt gatcgcggga atgcgccagg
      301 gcgagcactt ctatgaccag aaggcccccg acggaaaatg gtccttcact ggttcaaagg
      361 gattgcgtct tacctatggg ttcgatgaaa acctggttga ttacacgtgg ctttacgcct
      421 atccagcccg cgacggcgaa ctggaaatgg aagtgtttgc cagggcggtt acgctggcgc
      481 cgggtgaatc gttcacgttc atgacctgga tcgagattga accggccggg tcgaagtaac
      541 catcaggccg ttcgggcagg ccggatgcct tcgcgccggc aagtgtcacc actgaaaaca
      601 ttatttcgag acgaccttga aaactgtttc aggaagtctt tacaatagga cgtaagttcc
      661 tttaattttt ttgaattgcc cccgtgcggg gcgttcatgg aggttcggga tgcgaaacgt
      721 actgttggcg cttctttcgc tgtccgtcgt cgcgaccttt gccggttgcg agtatccgac
      781 ggttcaggat ggtgtggatg ggccggcgct gggaaccttc aatacgtccg gatgcaagct
      841 ggacaatgcc agtcagcgtt cgctgatgga cggcaacatg atcgaggccg tcgtctttga
      901 gacgtcggtg accatcattc atcatgatgc ccagtaccag tgcaacgccg aaatcggctg
      961 ggaactggaa gtcaacggca ccacgctgat cctgcgcgag gtcgataaat cgacggtcgt
     1021 gaccaggtgc atgtgcccga tggatctttc gaccacgatt gaaaacctgg ccgccggcgg
     1081 caagtacacg attcaggtct gggatgagtt ccagaccagg atgttcggcg aagtcaccgt
     1141 cgatctgggc gactgcaacg agcagtgcgt cacggacgag gattgctggg cctatccgga
     1201 tctgccgcgt ctcgactgcg agggccggtg ggcctgtatc aaggggctgt gcaattacgc
     1261 ctgcgaaccc tacgatcccg gatgcaccag cgattacgat tgtccggaag gcttccagtg
     1321 cgtgttctac agcaacgggg gcggatcggc cgagaccagt ccagacggca cgatggccca
     1381 gcgcatgatg gcgtacgagt gcaagagcaa cgaagactgc ccggaaggca tggtctgcga
     1441 gatgaccgac tgcgcgtgcc cgcccggggc cgattgtgac tgctatccct atggatactg
     1501 caccggcgga caggtatggc cgaccgaggg ttattgcgag ccgatcgtga tcaacaacga
     1561 gtgccgttcc gacgcggact gcggatccgg ttatcactgc gagttc
//
LOCUS       DAOPPA010000599         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_83204_length_1604_cov_3.729503, whole genome shotgun sequence.
ACCESSION   DAOPPA010000599 DAOPPA010000000
VERSION     DAOPPA010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83204_length_1604_cov_3.729503"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKH54_13520"
     CDS             complement(<1..449)
                     /locus_tag="PKH54_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HOD00961.1"
                     /translation="MANCSSCGKAAPEGSRFCPFCGGRIVIERQKPTDPLIGKIIGDK
                     YIVKDLIGSGAMGSIYRAEHKDLSRPVALKVLHRHLLTDEGQVHRFHREAKAASRLHH
                     PNAIGILDFGRTVDGWFYIAMEYLNGKDLSRMTNKEGAMSVEKVIHIA"
     gene            553..1320
                     /locus_tag="PKH54_13525"
     CDS             553..1320
                     /locus_tag="PKH54_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD00962.1"
                     /translation="MIRCVFCGTENDEIFTYCLNCGKPLEQSMQTFKAKAPASAPVEN
                     VKLVIVKSDGTDGSSFPLKAGENVLGRGPVDIDLGVDPRIASRHAVIDVGRDVAFIDG
                     VDMTFGSFVRLREPRVLADRDRVRIGHAVLEYRTGLQRPQGGAADVETLGSSAAQTGN
                     VFGRILRVGPNDTLLGGWLLNKAETLVGRTQGDIVLGQDGFVSSRHAIFAVNGNECSI
                     KDQNSTNGTFLMVRGRVAINNGDQILIGPHLLRFQRG"
     gene            complement(1308..>1604)
                     /locus_tag="PKH54_13530"
     CDS             complement(1308..>1604)
                     /locus_tag="PKH54_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOD00963.1"
                     /translation="LVRKQAKEHGTGSRVEGRFGLPAGADVLLLEDVLTTGISLTEAR
                     DGVIADGYNPVACMVLVDRDEGGRQTLEATGLKVIPVFSASEVAAKYDRLNQPR"
BASE COUNT          315 a          479 c          464 g          346 t
ORIGIN      
        1 gcgatgtgga taaccttctc caccgacatc gccccttcct tgttggtcat gcgggaaagg
       61 tccttgccgt tcaagtattc catcgcgatg tagaaccagc catcgacagt gcgcccgaaa
      121 tccaggattc cgattgcgtt ggggtgatgc agtctcgatg ccgccttggc ttcacggtga
      181 aagcgatgta cctggccctc gtctgtaagc aggtgcctgt gcaggacctt cagcgcgacg
      241 ggcctggaaa ggtccttgtg ctcggcccga tagatgcttc ccatcgcccc gctgccgata
      301 aggtccttta caatatactt gtcacctata atcttgccga tcaggggatc cgtcggcttc
      361 tgacgttcga taacgatgcg accgccgcag aaaggacaga aacgactgcc ctcgggagcg
      421 gcctttccgc aggatgaaca atttgccaag tcgatatacc ccgatgttgg tctttgcaat
      481 tctattcctg ggtgctagca ttggcaaggc gttacaagcc attaatccga tccatccctg
      541 gaggtgccgg ttatgatccg ctgtgttttc tgcgggaccg aaaacgacga gattttcacg
      601 tactgcctga actgtggaaa gccccttgaa cagtcgatgc agaccttcaa ggcaaaggct
      661 ccggcgtccg cgccagtcga gaatgtaaag ctggtcatcg tgaaatcgga cgggacggac
      721 ggatcgtctt tccccctgaa ggcgggcgag aacgtactcg gtcgcgggcc cgtcgatatc
      781 gaccttggcg tcgatcccag gattgcatca cggcacgcgg tcatcgatgt cggacgggac
      841 gtcgctttca tcgacggcgt cgatatgacc ttcggttcct tcgtccgtct gcgcgagccg
      901 cgcgtcctgg ccgatcgcga ccgggttcgc atcggccacg cggtgcttga ataccggact
      961 ggccttcaaa gaccccaggg cggtgccgcc gatgtggaaa cccttggttc gtcggcggcc
     1021 cagaccggca acgtcttcgg ccggattctt cgcgtcggcc cgaatgacac ccttcttggc
     1081 ggatggcttc tgaacaaggc cgaaacgctt gtcgggcgga cccagggcga tatcgtgctc
     1141 ggccaggacg gattcgtttc ttcgcgccac gcgatattcg ccgtaaacgg caacgagtgc
     1201 tcgatcaagg atcaaaactc gacgaacggc acattcctga tggttcgcgg ccgggtcgcc
     1261 atcaacaacg gcgaccagat cctgatcgga ccgcatcttc ttcgctttca gcgcggctga
     1321 ttgagcctgt cgtacttcgc ggcaacttcg gaagcagaaa agaccggaat taccttcaag
     1381 ccagtcgctt ccagtgtctg gcggccgcct tcgtcgcgat cgaccagaac catacaggca
     1441 accgggttat aaccatcggc gattacgccg tcgcgggcct cggtcaggga gatcccggtt
     1501 gtaaggacgt cttccagaag aagtacgtcg gcgccggctg gaaggccgaa tcgtccctcg
     1561 acacgggatc cggtgccatg ttcctttgcc tgcttgcgaa ccag
//
LOCUS       DAOPPA010000600         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_83415_length_1601_cov_3.578266, whole genome shotgun sequence.
ACCESSION   DAOPPA010000600 DAOPPA010000000
VERSION     DAOPPA010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83415_length_1601_cov_3.578266"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PKH54_13535"
     CDS             <1..1260
                     /locus_tag="PKH54_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOD00964.1"
                     /translation="DDSSPFASFQYMPAFLSSGIQPAPLRMPLSAAVYRFPGLSTATF
                     HGLPGMLADSLPDRFGNAVIDAWLTRNGRSPDSFNAVERLCYTGTRGMGALEYHPLVD
                     LGTSGDSALQVDALVRLAAGILSSRQGFVGRLGGPDGDTVSAESMAQILRVGTSAGGA
                     RAKAVIAWNPETGEVRSGQADTAAGFQQWLMKFDGVSGNADRELADPAGYGLVEYAYW
                     MMARAAGIVMSEARIYSENGRNHFMTRRFDRTGTGGKLHMQSLGAIGHHDYNAPGSCS
                     YEQAFGVIRALGLGMDAIEQFFRRMVFNIVARNQDDHVKNVAFLMDRSGTWLLAPAFD
                     VTWAYQPDGVWIGTHQMSMNGKRDGFVPEDFKACARTAGLVRGRGEEIVRDICKVVGN
                     WPEYAATAGVPQEWAESIFRTFRFFRA"
     gene            complement(1302..>1601)
                     /locus_tag="PKH54_13540"
     CDS             complement(1302..>1601)
                     /locus_tag="PKH54_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOD00965.1"
                     /translation="RTPTLIEEDCTIGHGAIIHGCIIRSGSLIGMGAIVLDDTEIGSE
                     SLIGAGCLVPERQKIPEGSLVIGVPGKVVRQLTTEERAAVRASAHHYVAYAAEFK"
BASE COUNT          279 a          477 c          530 g          315 t
ORIGIN      
        1 gacgacagca gtcctttcgc gtccttccag tacatgccgg ctttcctgtc cagcggaatc
       61 cagccggcgc cgctgaggat gccgctctcg gccgcggtct atcggtttcc cgggctttcg
      121 accgcaacat ttcacgggct gccgggcatg ctggcggact cgcttccaga caggttcgga
      181 aacgcggtga tcgacgcctg gctgaccagg aacggtcgaa gcccggattc cttcaacgcc
      241 gtcgagcgtc tttgctacac cgggacgcgc ggaatgggcg cgcttgagta ccatcccctg
      301 gtcgatctgg gcacgtcggg tgattctgca ttgcaggtgg acgcgctggt tcgcctggct
      361 gccgggatcc tgtcaagccg ccaggggttc gttggacgtc ttggcgggcc cgatggggac
      421 accgtcagcg ccgaatcaat ggcccagata cttcgtgtcg gcacgtcggc cgggggggcc
      481 agggcgaagg ctgtcatcgc gtggaatccg gaaacgggcg aagtcagatc cggacaggcc
      541 gataccgctg ccggcttcca gcagtggctg atgaagtttg acggggtgtc gggcaacgcc
      601 gaccgcgaac tcgccgatcc tgccggttac ggtcttgtcg agtacgcgta ctggatgatg
      661 gcgcgtgccg ccgggatcgt tatgagcgag gcgcgaatct attccgagaa cggccggaat
      721 catttcatga cgcggcggtt cgaccgaacc gggaccggtg gcaaactgca catgcagagt
      781 ctgggcgcca tagggcacca tgactacaac gcgccgggat cctgctcata cgaacaggcc
      841 tttggcgtga tccgcgccct tggtctcggg atggatgcga tcgagcaatt cttccgccgc
      901 atggtgttca atatcgtcgc caggaaccag gacgaccacg tcaagaacgt cgccttcctg
      961 atggatcgat ccggcacatg gctgctggcc ccggcgttcg acgtcacctg ggcgtaccag
     1021 cctgacggcg tctggatcgg cacccaccag atgtcgatga atggtaagcg ggacggattc
     1081 gtaccggagg atttcaaggc gtgtgccagg accgccggac ttgtaagagg gcggggcgaa
     1141 gagatcgtca gggatatctg caaagtcgtg gggaactggc ctgaatacgc ggccactgcc
     1201 ggcgtgccgc aggagtgggc cgaatcgatc ttcaggacat tcaggttttt ccgtgcctga
     1261 attcagtttc tgcggacttc gtcagctccc gccacggatt attacttgaa ttcggcggca
     1321 taggcgacgt agtggtgggc gcttgcgcgc acggccgcgc gttcctcggt ggtaagctgg
     1381 cggacgacct tgccgggcac gccgatgacc agcgagcctt ccgggatctt ctggcgttcg
     1441 ggaaccaggc atccggcgcc aatcagcgat tccgatccga tctccgtgtc gtcaagcacg
     1501 atggccccca tcccgatcag gctgccactg cggatgatgc atccgtggat aatggcgccg
     1561 tggccgatcg tgcagtcttc ctcgatcagt gtcggcgtgc g
//
LOCUS       DAOPPA010000601         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_83735_length_1597_cov_2.855383, whole genome shotgun sequence.
ACCESSION   DAOPPA010000601 DAOPPA010000000
VERSION     DAOPPA010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83735_length_1597_cov_2.855383"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1597
                     /locus_tag="PKH54_13545"
     CDS             <1..>1597
                     /locus_tag="PKH54_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00966.1"
                     /translation="ASSNPNVLIYTDTTDEETIGAATLDTTSYRVQKRDFWMTGLDPK
                     DVAVGDIDGNNVPDVAVLCAGANMVQVFFGNIQKEGARFFREGVALLAGAARDVALAD
                     LDGEGHLDLVLLNREGTALTAILWQSSELGNRFLGSYQAILDATPLSMQLGHFTAPLD
                     GSAAAFLDAAVLMPSQAGNPRVSVYASDPVIALPVSPAGSTSVDLNSPRKLVVGNYDV
                     RLNFPLEDSYKRPDDLIVTSDDTASAQNDPTAYALLFHEATHLSVSVSPVELAFGDKP
                     AILATGDIDRDASDDAYKVNDAIYVSRVEPESGNDGKWVLQTRLGQLNGTFVPRTIND
                     EPIPVMDMEDERNPVAIVSFPMHRTLNNYVKKTPGLIDVIVANGGTGDLTVFVNKGLS
                     VLTGKDEGSRDFALGGIPVDIAPGYLQSPVDGTAAPSVGSDQFADIVALLSDKIVILY
                     SLGAAKLGQLGEVVGYEPPVPLAFPGRIPVNVEVADMNGDGYMDIVVLDQDSSAVWIY
                     VNLGKRRFSPPFMFDTGTLPVEMF"
BASE COUNT          319 a          449 c          483 g          346 t
ORIGIN      
        1 gccagttcca atccgaacgt cctgatatac accgacacca cggacgagga gaccatcggc
       61 gcggccaccc tggacacgac gtcgtatcga gttcagaaac gtgatttctg gatgaccgga
      121 cttgatccga aagacgtggc ggttggcgat atcgacggca acaacgtgcc tgatgtcgcg
      181 gtactttgtg ccggcgcgaa catggtccag gtgtttttcg gaaacataca gaaggaaggc
      241 gccaggttct tcagggaagg ggttgccctg cttgcgggag ccgcccgcga cgttgccctt
      301 gcagacctgg atggcgaggg acatctggat ctggtgcttc tgaacaggga aggaacggcc
      361 cttaccgcca tactctggca gtcttccgaa ctgggcaacc gatttctcgg gtcataccag
      421 gcgatcctgg acgccactcc gctgtcgatg caacttgggc acttcaccgc gccacttgac
      481 ggcagcgctg cggcattcct tgatgcagcg gtgctcatgc cttcgcaggc tggcaaccca
      541 agggtcagcg tgtatgcgtc cgatccggtc attgcccttc ccgtcagccc ggccggcagc
      601 acgtcggtcg atttgaattc gcccaggaag ctggttgttg gaaattatga cgtgcgtctg
      661 aattttcccc tggaggattc gtacaagcgg cccgatgatt tgatagttac gtcggacgac
      721 accgcatcgg cgcagaatga tccgaccgcg tacgcgttgc ttttccacga agcaacccat
      781 ctgtcagtgt cggtcagtcc ggtcgagctt gcattcggtg acaagccggc catcctggct
      841 accggtgaca tcgatcgtga tgccagcgat gacgcataca aggtcaacga cgccatctat
      901 gtgtcgcgtg tcgagccgga atcgggtaac gacggcaagt gggttctgca gaccaggctt
      961 ggccagctta acggcacgtt cgtgcccaga accatcaatg atgagccgat tccggtcatg
     1021 gatatggaag acgaaaggaa tccggtggcc attgtttcgt tccccatgca tcggaccctg
     1081 aacaattacg tgaagaagac gcccggcctg atcgatgtca tcgtcgcaaa tggcggaacg
     1141 ggcgatttga cggtattcgt caacaagggg ctcagcgtgc tgaccggaaa ggatgagggc
     1201 agtcgtgact tcgcgcttgg cgggattccg gtcgatatcg cgcccgggta ccttcagagt
     1261 ccggttgacg gcacggcagc tccgtctgtc ggcagcgatc agttcgccga cattgtcgcg
     1321 ttgctttccg acaagatcgt gattctgtat tcccttggcg ccgccaagtt aggccagctt
     1381 ggcgaggtgg tcggatatga accgcccgtg ccgctcgcgt ttccgggcag gataccggtc
     1441 aatgtcgaag ttgccgacat gaacggcgat ggatatatgg atatcgtcgt gctggatcag
     1501 gattcctcgg ctgtctggat ctacgtcaac ctgggcaagc gcaggttcag cccgccattc
     1561 atgtttgaca ccggcaccct tccggtcgag atgttcg
//
LOCUS       DAOPPA010000602         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_83794_length_1596_cov_3.690461, whole genome shotgun sequence.
ACCESSION   DAOPPA010000602 DAOPPA010000000
VERSION     DAOPPA010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83794_length_1596_cov_3.690461"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1516)
                     /locus_tag="PKH54_13550"
     CDS             complement(41..1516)
                     /locus_tag="PKH54_13550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD00967.1"
                     /translation="MRGEPAVVLRSRRLSSRLPVARILLVKPRFMGPEFRSITHPMGL
                     MYVAATLRQAGHEPRIIDCAADPYDFDGPARIAADWRPDCIGLSIIVSELEQASGIIG
                     SIRARSPEIPIVLGGPWPSANPADALIMLQADFVVAGEGELTFPLLVDAIERRQNPFV
                     TAKSIPGVAARIGDSVQGPSPAPLPDLDRLPLPAWDLLDHELYARTLSMAGVGCRPYM
                     TVVTSRGCPFRCAYCHQTQGKQFRRRTAQSVVDELVTLNRDHGFSEFEIVDDCFNLDR
                     ERMHAILNGIIDAIPDARLHFPNGVRADHLDGRDIRLLRQAGTVSACFAIETASPDLQ
                     KVIRKNLDIDKALDAIHAAVAEGIYSTGFFMLGLPGETGSQAMATVEFAARSPLHRAI
                     FMLTTPFAGTELADMAARITGTPITLNAVRGLNYFTSSINISAMTDDELNSIFRSAYR
                     RFYLNPRRILRVISQHPRKQSLPRYALIMIEKIIPGRKRTR"
BASE COUNT          319 a          445 c          512 g          320 t
ORIGIN      
        1 gcggaagtcc agcaagcaga atagtcgata acgccatgcg tcagcgtgtt cttttccgtc
       61 ctgggatgat tttttcaatc atgataaggg catagcgtgg aagcgactgc ttcctggggt
      121 gctgggaaat gacgcgaagg atgcgtcgcg ggttcaggta gaaccttcgg taggcagatc
      181 tgaagatact gttcagttca tcgtccgtca tggccgagat gttgatgctg ctggtgaagt
      241 agttcaggcc gcgaaccgcg ttcagggtta tcggtgtccc ggtgatccgg gcggccatgt
      301 ccgccagctc cgttccggca aacggagtcg tcagcatgaa gattgccctg tgaaggggcg
      361 aacgggcggc gaactcgacg gtggccatcg cctggctgcc ggtttcgcct ggcagtccga
      421 gcatgaagaa cccggttgaa tagatgcctt cggcaacggc ggcatggatg gcgtcaaggg
      481 ccttgtcgat gtcgaggttc ttgcggatga ccttctgaag atccggtgac gccgtctcaa
      541 tcgcgaagca ggccgacacg gttcccgcct gccgaagaag ccttatgtcc cgcccgtcca
      601 ggtggtcggc gcgcactccg ttcgggaaat gcagcctggc atcggggatc gcgtcgatta
      661 tgccgttaag gatggcatgc attctttcgc gatccagatt gaagcagtcg tcaacgattt
      721 cgaattcgga aaagccatgg tctcggttca gggtgacgag ttcatcgaca accgattgag
      781 ccgtcctgcg ccggaactgc ttgccctggg tctggtggca atatgcgcac ctgaacgggc
      841 aaccccttga agttacgacg gtcatgtagg gtctgcaccc gaccccggcc atggagagcg
      901 tccgcgcgta aagttcgtga tccagcagat cccaggcggg aagcggcaga cggtcgagat
      961 cgggcagggg ggcgggcgag gggccctgga cgctgtcgcc gatccgtgcg gccacgccag
     1021 ggatggattt cgccgtcaca aaggggttct gccgcctctc aattgcatcg accagaagcg
     1081 gaaaggtcag ttcgccctcg ccggcaacga cgaagtcagc ctggagcatg ataagcgcgt
     1141 ccgccggatt tgccgacggc cacgggccac cgaggacgat cgggatttcc ggactgcggg
     1201 cccttatcga accgataatg cctgatgcct gttcaagctc ggaaacgatg attgacaggc
     1261 cgatgcagtc aggccgccag tctgcggcaa tccttgccgg gccatcgaag tcgtaagggt
     1321 ctgccgcgca gtcgatgatg cgcggctcat ggccggcctg tcgaagcgtg gcggccacgt
     1381 acatcagccc catcgggtgc gttatcgacc tgaactctgg gcccatgaac ctgggcttga
     1441 caagcagtat ccgtgccaca ggaagccttg aagacagcct tcgagatctt agcacaaccg
     1501 caggttcgcc gcgcattcgg cccgaatatc gataccgttg tacaatcgcc tcgtactgga
     1561 accggggtca tcgcatgagg acattttcgg gaatag
//
LOCUS       DAOPPA010000603         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_84396_length_1588_cov_2.632094, whole genome shotgun sequence.
ACCESSION   DAOPPA010000603 DAOPPA010000000
VERSION     DAOPPA010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84396_length_1588_cov_2.632094"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PKH54_13555"
     CDS             complement(<1..1461)
                     /locus_tag="PKH54_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00968.1"
                     /translation="MKALPVLAAAVLLIFVVSCEKGGESGSCGSEQAPVAAVDSNPSD
                     RGGDPVEKLPPLSRDDFNRLAAAADSSVYWVSDQVDPGVLNPDELVVTGVSGDLGKWV
                     RDGAFTADFDRLIRDLSERRRQEAVARELAQGRPTLVMSDLSGLSPDEKQLVTHVLNA
                     AAMTDRLHRLQRGAATYEKDLASWRSDDRALFMRNNGPWCEAPGTADDPFCNAGSGFP
                     GQVYTTYPADEKHELELCTRLQALPNATELMDPFTVVRKAEDGSFVAVPINKVYDGPM
                     NEVAAELDKAAELAKKVGEDAFATYLAAAAGSFRSNDWNPADEAWVAMNATNSKFYLR
                     IGPDEVYWDLCQQKAGFHTSFALIDKGSLELQDKLNPLRSRMEESLGELTADYKPRQV
                     SFQMPDFIQIIMNAGDARKGIGATIGQSLPNWGSVVERGGSRTVVMTNLYQDPDSREL
                     AKKKAAELLAPDTMAFYAEEQRIALIDIILHEATHNL"
BASE COUNT          316 a          495 c          465 g          312 t
ORIGIN      
        1 aaggttgtgg gtggcttcat gaaggatgat gtcgatcagg gcaatcctct gctcttccgc
       61 gtagaaggcc atcgtgtcgg gcgcaagcaa ttccgcggcc ttcttcttcg ccagttcgcg
      121 gctgtccgga tcctggtaca ggttggtcat gacgaccgtc ctgcttccac cacgttcgac
      181 gacgcttccc cagttgggca gggactggcc gattgttgcg ccgattccct tgcgcgcatc
      241 gccggcattc atgattatct gaatgaaatc gggcatctgg aaactaacct ggcgcggctt
      301 gtagtcagca gtcagttccc cgagcgattc ctccatcctt gaacgcagcg gattcagctt
      361 gtcctgcagt tcaagcgagc ccttgtcgat caaggcgaag gaagtgtgga aaccggcctt
      421 ctgctggcac aggtcccaat agacttcgtc cgggccaata cgaaggtaaa acttcgagtt
      481 cgtggcgttc atggcaaccc aggcctcgtc ggccgggttc cagtcgttgc tgcggaatga
      541 tccggccgcc gccgcaagat acgtcgcgaa ggcatcctcg ccgaccttct tcgcgagttc
      601 ggccgccttg tccagttcgg ccgcgacctc gttcatcggc ccgtcataaa ccttgttgat
      661 cggcacggcg acgaaagacc cgtcttccgc cttgcgcacg acggtgaacg gatccatcag
      721 ttccgtggcg ttcggcaggg cctgcaacct ggtacagagt tccaactcat gcttttcgtc
      781 ggcgggatag gtcgtataga cctgacccgg gaagccggac ccggcgttgc agaagggatc
      841 gtcggccgtc cccggggcct cgcaccatgg accgttgttc cgcatgaaca gggcgcgatc
      901 atcggatcgc catgaagcca aatccttttc gtaagtcgcc gccccgcgct gaagcctgtg
      961 aagccggtcg gtcatcgcgg cggcgttcag gacgtgcgtg acaagttgct tctcgtcggg
     1021 cgaaagaccg gacagatcgg acatgacgag cgtcggacgg ccctgggcca gttccctggc
     1081 gaccgcctcc tggcggcgac gctccgacag atcgcggatc agccggtcaa aatcggcagt
     1141 aaaggcgccg tccctgaccc actttccaag atccccggaa actccggtga cgaccagttc
     1201 atccgggttc aggacaccgg gatcgacctg gtccgaaacc caatagacag aactgtcggc
     1261 ggccgcggcc agcctgttga aatcgtcccg ggaaagcggc ggaagcttct cgaccggatc
     1321 tccgcccctg tcggaaggat tggaatcaac ggcggcgacc ggcgcctgtt cagacccgca
     1381 ggaaccggat tccccgccct tttcgcagga gactacaaaa atcagcagca cggctgccgc
     1441 aagaaccggt aacgctttca tgaggtcctc caaaaggtac tgccttgatt ccgcgatcca
     1501 gaccgcggac atcggatatt aacccgaagc acgcacacta taaataccaa acgggactac
     1561 gatatgatcg ggcatccccg acggtggg
//
LOCUS       DAOPPA010000604         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_84868_length_1581_cov_10.650066, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000604 DAOPPA010000000
VERSION     DAOPPA010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84868_length_1581_cov_10.650066"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="PKH54_13560"
     CDS             complement(<1..>1581)
                     /locus_tag="PKH54_13560"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOD00969.1"
                     /translation="YGAQNKNSTIGRMVLPSDSTDPDDILARNAQVKASLLSARPHGG
                     TPLGAMFYDTLTMIRSETEFVDDPFATCRNKHVVLITDGRSNTGEDDGGYPDPVEGAQ
                     ELRNEGYEVWVIQFGVAEGVTSQGYDIATYHGLLADDHFLMADDAASLASALGSVVSV
                     NATSPQSRTGIVITEQTGNTTDVQYQFNAAYRNVNAPAGAADVDVLSGILERSVYQCG
                     VDVDQPDTAGLALIQSIGDKLNLTSDGSRNIYTMVKGDLESFVWTNASITNDMLDAPN
                     PLVEPVPNFGLDDDTDWCAQGTIQGLAIQKLLKWRQNLFSYIRADDESCRSGHKLGGI
                     VHSTPAFQDTLKGVSLSIPSFASYKASIASRPHMLYTCTHDGMLHAFRTDRPNGNAEP
                     DSWGKELWAYIPAHQLDVLRTVPELGEVALDGAPVFADIVLSRTFTSRATENEDSWKS
                     VLLFGDRAGGAGYTALDVTDPTDGNWSVLWEISAEEGRCPGGSNNCNSGGMGSFRNNY
                     SRLGKSYGKPVIGTVMIC"
BASE COUNT          296 a          464 c          482 g          339 t
ORIGIN      
        1 gacagatcat gaccgtgccg atcaccggtt tgccatagga cttgcccagc ctggagtagt
       61 tgttccggaa gctgcccatg ccgcccgagt tgcagttgtt cgaaccgcct ggacaccggc
      121 cttcctctgc cgaaatttcc cacaggaccg accagttgcc atcggtggga tcggtcacgt
      181 ccagcgccgt gtatccagct ccgcccgccc tgtcgccgaa cagaagcacg gatttccagg
      241 aatcttcgtt ctcggtagcc cggctggtga acgtcctgga taggactatg tcggcgaaaa
      301 ccggtgcgcc atcgagtgca acctcgccca gttcggggac ggtgcgcagc acatcgagct
      361 ggtgagcggg tatgtaagcc cagagttcct ttccccagga atcgggttcc gcgtttccgt
      421 taggacgatc ggttcggaac gcgtgaagca tgccgtcgtg agtgcatgta tagagcatgt
      481 gcggtcggga cgcgatcgat gccttgtagg aggcaaaaga cggaatcgaa agacttacgc
      541 ccttcagcgt gtcctgaaag gcaggggtgg agtgaacgat cccaccaagc ttatggcccg
      601 acctgcacga ttcatcgtcc gcgcgtatgt acgagaacag gttctggcgc cacttcagca
      661 atttctggat cgcgagtccc tggatggttc cctgggcaca ccagtcggtg tcatcgtcca
      721 gaccgaagtt cggcaccggc tcgacgagcg gattcggagc atcaagcatg tcgttggtga
      781 tactggcatt ggtccatacg aaactctcga gatcgccttt gaccatggtg tagatgttgc
      841 gtgacccgtc cgacgtcagg ttcagtttgt ctccgatgct ctggatcagg gcaaggcccg
      901 cggtgtccgg ctggtccacg tccacgccgc actggtagac ggaacgctcg aggatgcccg
      961 aaagaacgtc gacatccgcg gcgccggccg gcgcgttcac gttccggtaa gcggcgttga
     1021 actgatactg cacgtcggtc gtgttgccag tctgttcggt gatgacgatc ccggttctgg
     1081 actgtggtga cgtcgcattg accgacacca cgcttccgag cgcgctggcc agcgaagcgg
     1141 cgtcatcggc cattagaaag tgatcgtccg caagaagccc atgatacgtc gcgatgtcat
     1201 atccctggct ggtaacaccc tcggcgacgc cgaactggat gacccagacc tcgtaacctt
     1261 cattgcgaag ctcctgggcg ccttctactg gatccgggta tccaccgtcg tcttcacccg
     1321 tgttgcttct tccatccgtg atcaggacca catgcttgtt tcggcaagtg gcgaacggat
     1381 cgtccacgaa ttccgtttcg gaacggatca tcgtcagagt gtcgtaaaac atggcgccga
     1441 ggggcgtgcc gccgtgcggc ctggccgaca gcagggatgc cttgacctgg gcgtttcggg
     1501 cgaggatgtc gtcgggatcc gtcgagtccg acggcaggac catcctgcca atcgtcgagt
     1561 ttttgttctg ggcgccgtag t
//
LOCUS       DAOPPA010000605         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_85294_length_1576_cov_2.928994, whole genome shotgun sequence.
ACCESSION   DAOPPA010000605 DAOPPA010000000
VERSION     DAOPPA010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85294_length_1576_cov_2.928994"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKH54_13565"
     CDS             <1..1300
                     /locus_tag="PKH54_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOD00970.1"
                     /translation="PAAGVRISQIANLADDLTMALKATSIRIVAPIPGKGVVGIEVPN
                     KVRETVYLREILASEEFTKSRSKLTIALGKDIFGNPVVTDLARMPHLLIAGATGSGKS
                     VAVNAFILSLLRNATPDEVRIILVDPKVVELNVYENIPLLLLPVVCDPKQAAAALRWA
                     CEEMDRRYSLLARFSARNIASFNARIDRLLSLKAGKSAGTGDDDADGLASLDPDEFDI
                     DELVRLPYIVIVLDEFADLMMVASKDVEAAVTRLAQKARAAGIHLVMATQRPSKEVIT
                     GLIKSNFPSRIGFRVSSSIDSRIILDQSGAEALLGFGDMLFLKPGTSALERVHGSFVS
                     DDEVMRVVTHLKTQGEPDYQLDIIKDADDVEIQEDQDEDPLTDEAQDIVLESGKASIS
                     FLQRRLKIGYNRSARIMEALERRRVVGPPDSRGERRVIEL"
     gene            complement(1318..>1576)
                     /locus_tag="PKH54_13570"
     CDS             complement(1318..>1576)
                     /locus_tag="PKH54_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00971.1"
                     /translation="EGDAALADFTTWPGEPAPTVVAELDAGSPAEFRFTVSLGNDEMG
                     YMVLEKDWNNVEYGYERSMSLGKHATTEVMQHINALRDLLK"
BASE COUNT          343 a          483 c          463 g          287 t
ORIGIN      
        1 gccggcagcc ggagtcagga tcagccagat cgcgaacctg gctgacgacc ttacgatggc
       61 cctgaaggcg accagcataa gaatcgtggc gccgattccc ggcaagggcg tcgtcggcat
      121 cgaggtgccc aacaaggtaa gggaaactgt gtatcttcgg gaaatcctgg caagcgagga
      181 attcacgaag tccagaagca agctgacgat tgccctcggc aaggacatct tcggcaaccc
      241 ggttgtcacg gatctggcaa ggatgcccca cctgctgatc gccggcgcga ccggctccgg
      301 aaagtcggtc gccgtcaatg cgttcatact gagcctgctt cgcaacgcca cgccggatga
      361 agtcaggatc atcctggtcg atccgaaggt cgtcgaactg aatgtctatg aaaacatccc
      421 gctcctgctg cttccggtag tctgcgaccc gaagcaggcc gccgccgcac ttcgctgggc
      481 atgcgaggaa atggatcgcc ggtacagcct gctggcaaga ttctcggcca ggaacatcgc
      541 gtctttcaac gcgcgcatcg accgtctgct gagcctaaag gccggcaagt cggccgggac
      601 cggcgacgat gacgccgacg gactggcatc gcttgacccc gatgaattcg acatcgacga
      661 actggtaagg cttccctata tcgtcatcgt cctggacgaa tttgccgacc tgatgatggt
      721 cgcatcgaag gacgtggagg ccgccgtaac acgtctggcc cagaaggccc gcgcagccgg
      781 cattcacctg gtgatggcga cccagcgccc atccaaggaa gtcatcacgg gcctcatcaa
      841 gtccaacttc ccatcaagga tcggcttccg ggtctcgtcc agcatcgaca gccgcatcat
      901 actggatcaa tcgggcgccg aggctctgct tggattcggc gacatgctgt ttctgaagcc
      961 tggcacttcc gcgctggaac gcgtccacgg ttcattcgtg tcagacgacg aggtcatgcg
     1021 tgtcgtgacc cacctgaaga cccaggggga acccgactat cagcttgaca tcatcaagga
     1081 cgccgacgac gttgaaatcc aggaagacca ggacgaagac ccgctgaccg acgaagccca
     1141 ggacatcgtt cttgaaagcg gcaaggccag catttccttc ctgcagcgca ggcttaagat
     1201 cggttacaac cgaagcgcca ggataatgga agcgctggag cgccgaaggg tggtcgggcc
     1261 gcccgacagc cgcggggaaa ggcgggtaat cgagctctga tcggccggct cggtgattta
     1321 cttcaaaaga tcgcgcaaag cgttgatgtg ctgcatcact tccgtcgtcg cgtgcttgcc
     1381 aagagacatg ctccgctcgt aaccgtactc gacgttgttc cagtcctttt ccagaaccat
     1441 gtatcccatc tcgtcgtttc caagcgaaac cgtgaagcgg aactccgcgg gcgacccggc
     1501 atccagttcg gcaacaacag tcggagccgg ctcgcctggc catgtggtga aatcggcaag
     1561 cgcggcatcg ccttcc
//
LOCUS       DAOPPA010000606         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_85497_length_1573_cov_3.318182, whole genome shotgun sequence.
ACCESSION   DAOPPA010000606 DAOPPA010000000
VERSION     DAOPPA010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85497_length_1573_cov_3.318182"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PKH54_13575"
     CDS             complement(<1..1524)
                     /locus_tag="PKH54_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00972.1"
                     /translation="MKSTARVTALILASMLLSCGGGGGQPDVSELPGDDVMPDVQLDT
                     APEILIPDTGDEPREEIFQETIDPDAIQDVPDEGGIDVPFEMPEVCECTQDEECEDAF
                     DDLGLCERASCDRCACVRVWLEADAACDDGDLCTIDDKCDADHVCQPGQNNCNCSTDS
                     DCTLYDDNNKCNGSYRCNLAVTPSVCHIPAETIPVCEDTLGLFCRTVGCDPETGDCIN
                     INIHQGEPCQDGVDCTTGDSCADGECVGVPDALLCDDGNVCTDNACDAQAGCTMVFND
                     GACQDGNACTENDFCLNGSCSAGTDVDCDDSNECTNDLCDTETGCIHENLVDTLCNDG
                     NDCTGGDYCMEGSCLAGFETCCEDQMDNDQNGKTDCEEESCKFACPVENISCIVESPT
                     THTGPSGDTFTAIVKVTAPGITDTTDKYDAHPKVHVQYGVGPEGADPAGLTWGDWLPA
                     TPDMDAVDASSDIWSGVVTVPAITDMSLTRDFAFRVSGDDGTTWIYCDTDGSDNGYAA
                     "
BASE COUNT          285 a          471 c          462 g          355 t
ORIGIN      
        1 ggccgcgtat ccgttgtcgc tgccatcggt gtcgcagtaa atccacgttg tgccgtcgtc
       61 gcccgatacc cggaacgcga agtcgcgcgt cagcgacatg tcggtgatgg ccggaacggt
      121 cacgacaccg ctccagatgt ccgaagacgc atcgaccgca tccatgtcgg gggttgccgg
      181 aagccagtcg ccccaggtca atcctgccgg atcggcccct tcgggtccaa cgccatactg
      241 gacatgaacc tttggatggg catcgtattt gtccgtcgtg tcggtgatgc cgggcgccgt
      301 caccttgacg atcgccgtga acgtgtcccc ggaaggtccg gtatgggtgg tcggggactc
      361 aacgatgcat gaaatattct caaccggaca tgcgaacttg caggattctt cctcgcagtc
      421 ggtcttgccg ttctggtcgt tgtccatctg gtcttcacag caggtctcga acccggccag
      481 gcacgatcct tccatgcagt agtcgccgcc ggtgcagtcg tttccgtcgt tgcacagggt
      541 atcgaccagg ttttcgtgaa tgcagccggt ttccgtgtcg caaagatcgt tggtgcattc
      601 gttgctgtcg tcgcaatcaa cgtcggttcc ggcagagcat gatccgttca ggcagaaatc
      661 gttttcggta caggcgtttc catcctggca ggccccgtcg ttgaagacca tcgtgcaacc
      721 ggcctgggca tcgcaggcat tgtccgtgca gacgttgccg tcatcgcaca gaagcgcgtc
      781 cggcaccccg acgcattcgc cgtcggcgca tgagtcgcct gtcgtgcaat cgacgccatc
      841 ctggcagggt tcgccctggt ggatgttgat gttgatgcag tcgccggttt caggatcgca
      901 gccgacagtc cggcagaaaa gaccgagagt atcttcgcag accgggatgg tttctgccgg
      961 aatgtggcag acagaaggtg tcacggccag gttgcacctg tacgaaccgt tgcacttgtt
     1021 gttgtcgtcg tacaacgtgc agtcgctgtc ggtgctgcag ttgcagttgt tctggcccgg
     1081 ctggcagaca tgatccgcat cgcacttgtc atctatggtg caaaggtcgc cgtcatcgca
     1141 tgccgcgtcc gcttcaagcc agacacgcac acatgcgcag cggtcgcagg atgccctctc
     1201 acacaggccg aggtcgtcga acgcatcctc gcattcctca tcctgcgtgc attcgcagac
     1261 ttcgggcatt tcgaagggaa catcgatgcc gccttcgtcc ggcacgtcct ggattgcatc
     1321 gggatcgatc gtttcctgga aaatttcttc cctgggctcg tcgccggtgt ccggaatcag
     1381 gatttccggc gcggtgtcca gctggacatc cggcataacg tcatctcccg gaagttcgga
     1441 tacatccggc tgaccgccgc cgccgccgca tgaaagcagc attgaagcaa ggatgagcgc
     1501 ggtgactctg gctgttgatt tcatctgttc ccccaaatga ttttcacaat gaccaaagga
     1561 tgataacccc gtg
//
LOCUS       DAOPPA010000607         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_85730_length_1570_cov_3.765017, whole genome shotgun sequence.
ACCESSION   DAOPPA010000607 DAOPPA010000000
VERSION     DAOPPA010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85730_length_1570_cov_3.765017"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="PKH54_13580"
     CDS             <1..>1570
                     /locus_tag="PKH54_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOD00973.1"
                     /translation="GGMGSVYLALQYPLEREVAIKTVLNQAFGDVALKRFMREAKSLG
                     DLDHPGIVKLFHFGIEKLEFSMPFMVMEYVRGGKWLDRFFQSERQEGRPVSPELVCRM
                     FEQVLEALDAAHSINIVHRDIKPANILVTGSGMDARAKILDFGLAKPIHEEANSDNLT
                     KSLETIGTPMYMAPEQAKSSLMSRVDGRTDLYSVGVMMFHVLTGVLPFRGGTPIEILM
                     QKADPTYDPLSLPQAADLSPGFRDFFRRVLASSPTRRPETAKKMAAEFKYLMTSDQTV
                     TTILNTGPHEAARQRFDSPLYYNRPDSSSYSDSSHPSDRNDPSIDQGTRRRRGGGGTG
                     DGLWKWLLFSGVVLCAAAMGLLVYLLFAGESGSVPTGPAPGVIAGAVTEQGQPPGQGA
                     YPAAGQQVQNPVVPQGQPVQNQQAGVVPAQPGGVPGQPATLQGAPLQGAPLQPVPGQA
                     VAVGQQAPQGQVAGQPGMIADTAGAAPVDPQPTQPTETADQAAQADSPAQADKPAETA
                     EKVEPVNESELEPFE"
BASE COUNT          291 a          463 c          521 g          295 t
ORIGIN      
        1 aggcggaatg gggtcggttt atctggcgct tcagtatccg cttgaacgcg aagtcgcaat
       61 caagacggtc ctgaaccagg ctttcgggga cgtcgcgctg aagcgtttca tgcgtgaagc
      121 gaagtcgctg ggcgatctgg atcacccggg tatcgtgaag ctgttccatt ttggcattga
      181 aaaacttgaa ttttcgatgc ccttcatggt gatggagtac gtccgcggcg ggaagtggct
      241 tgaccgcttt ttccagtccg agcgtcagga aggccggccg gtttccccgg aactggtctg
      301 ccggatgttc gagcaggttc ttgaagccct ggatgccgcg cattccatca atatcgttca
      361 ccgcgacatc aagccggcca atatcctggt gaccggaagc gggatggacg ccagggccaa
      421 aatcctggac ttcgggcttg ccaagccgat tcacgaggaa gccaattccg acaacctgac
      481 gaagtcgctg gaaacgatcg ggacaccgat gtacatggcg ccggaacagg cgaagtcgtc
      541 gttgatgtcc cgcgttgacg gaaggaccga cctgtactcg gtcggcgtga tgatgttcca
      601 tgtcctgacc ggcgttctgc cgttccgtgg cggcaccccg attgaaatcc tgatgcagaa
      661 ggcggatccg acctatgacc cgctttcgtt gccccaggcc gcggatttgt cgccaggttt
      721 ccgcgatttc ttccgtcgag ttcttgcttc gtcaccgacc aggcgtcccg aaaccgcaaa
      781 gaagatggcg gccgaattca agtacctgat gacttcggat cagacggtca cgaccatcct
      841 gaacacggga ccccacgagg cggcaaggca gcgattcgat tcgccgcttt attacaaccg
      901 gcccgacagt tcgtcgtatt cggattcatc ccatccttcc gaccggaacg atccgtcgat
      961 cgatcaggga acaaggcggc ggcggggtgg gggcggcact ggcgacggtt tgtggaagtg
     1021 gctcctgttt tcgggggtcg tcctttgcgc cgccgcgatg gggctgctgg tatatctgct
     1081 gtttgcgggc gagtcgggaa gcgtcccgac aggtccggcc ccgggcgtga ttgccggtgc
     1141 cgtgacagag cagggccagc cgcccggtca gggcgcgtat ccggccgccg gtcagcaggt
     1201 tcagaatccc gtggtgccgc aggggcagcc cgtccagaat cagcaggccg gcgtggttcc
     1261 cgcccagcct ggcggggtgc cagggcagcc ggcaactttg cagggtgcgc ctttgcaggg
     1321 ggcgcctttg cagccggttc cgggacaggc ggtcgccgtc gggcagcagg caccacaggg
     1381 gcaggtggct ggccagcccg gcatgattgc cgatacggcg ggcgcagccc cggtggatcc
     1441 gcagccgacg cagccgacgg aaaccgcgga tcaggcggcc caggccgata gtccggccca
     1501 ggccgataag ccggccgaaa ccgccgaaaa ggtcgaaccg gtaaacgaat cggaactgga
     1561 accgttcgag
//
LOCUS       DAOPPA010000608         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_86789_length_1556_cov_3.183877, whole genome shotgun sequence.
ACCESSION   DAOPPA010000608 DAOPPA010000000
VERSION     DAOPPA010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86789_length_1556_cov_3.183877"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKH54_13585"
     CDS             <1..182
                     /locus_tag="PKH54_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOD00974.1"
                     /translation="DFLYVTPVQGVVNARVITNPAHMKRIIRALQDNVAKYEAQFGHI
                     PEVVPAEPMPDMEPN"
     gene            192..>1556
                     /locus_tag="PKH54_13590"
     CDS             192..>1556
                     /locus_tag="PKH54_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOD00975.1"
                     /translation="MDWIDSRPLNRVACAVQELPHLLADTDAARHWAATTGGRRIKGV
                     CVPLFSLRSDRGCGIGDVGDLPGAIDYCARSGMELLQLLPINDSGLDGVPYSAMSAFA
                     IDPAFIAIDDIPGLDETFLLHVSSVTAPFREAGRIDNPRVRRLKIDLLRQAWDLVRND
                     ALISSLDAFCAENPWVVGYAAFKVVKDIEGLRPWEEWASVAGLSTDQVIKLARATPDF
                     NFHVFCQHTADRQFAAAHRYARSRGVLLEGDIPILVARDSADVWAERWMFHLDFAAGA
                     PPDMYAEDGQHWGFPTYNWDAIERDDYRWWRQRLRTAQRYFDCYRIDHIVGFFRIWTI
                     DVHAPNGRQGWFDPSDEAVWGDHGRKILGMMLDSTDMLPLGEDLGTIPHICRSTMLDM
                     GICGLKVQRWEKRWEGDRKFIAPADYLPVSVATLSTHDCELAPEWWRNADAADRQELW
                     ELLGG"
BASE COUNT          283 a          465 c          498 g          310 t
ORIGIN      
        1 tggacttcct gtacgtgacg ccggttcagg gcgtggtcaa cgccagggta atcaccaatc
       61 ccgcccacat gaagcgaatc atccgcgcgc tgcaggacaa cgtggcaaag tacgaggcgc
      121 agttcgggca cataccggaa gtggtcccgg ccgagccgat gcctgacatg gaaccgaact
      181 gaaggttgca gatggattgg atcgatagtc ggcccttgaa ccgggtggcg tgtgccgtcc
      241 aggagcttcc gcaccttctg gctgatacgg atgccgcccg tcactgggcg gcgacaaccg
      301 gcggacgcag gatcaagggg gtctgcgtcc ccttgttttc acttcgaagc gatcgtggtt
      361 gcggcatcgg cgatgtcggc gacctgcccg gggcaatcga ctactgtgcg cgttccggca
      421 tggagttgct tcaactgctg ccgatcaacg attccggcct ggacggcgtc ccgtactcgg
      481 cgatgtcggc gttcgcgatt gatcccgcct tcattgcaat cgacgacatc ccgggactgg
      541 atgaaacctt tcttctgcac gtatccagcg tgacggcacc ctttcgcgaa gccggccgaa
      601 tcgacaaccc cagggttcgc cggttgaaga ttgacctgct tcgacaggcc tgggatcttg
      661 ttcgaaacga tgcattgatt tccagcctgg atgcgttctg cgccgagaac ccgtgggtcg
      721 tcggatatgc ggctttcaag gtggtcaagg atatcgaggg gcttcggcca tgggaggaat
      781 gggcgtcggt ggcgggcctt tcgacggatc aggtgattaa gctggccagg gccacgcctg
      841 atttcaattt tcacgtattc tgccagcaca cagccgaccg gcagttcgcg gccgcacatc
      901 gctacgccag gtcacgcggc gtccttctgg aaggcgacat cccgatactt gtggcccgcg
      961 acagcgcgga cgtgtgggcc gagcgctgga tgttccacct ggatttcgcc gccggcgcgc
     1021 ccccggacat gtacgccgag gacggccagc actggggctt ccccacatac aactgggacg
     1081 ccatcgaacg tgatgactat cgctggtggc gtcaacggct gcgtactgcc cagcgttact
     1141 tcgactgtta ccgcatcgac cacatcgtcg ggttcttccg aatctggacc atcgacgtgc
     1201 acgcgccgaa cggacgtcag ggctggttcg acccgtcgga tgaggctgtc tggggcgatc
     1261 atggccgcaa gatcctgggg atgatgctgg attcaaccga tatgctgccg cttggcgaag
     1321 accttggcac cattccccat atctgtcgaa gcacgatgct tgacatgggc atctgcggcc
     1381 tgaaggtcca gcgctgggaa aaacgctggg aaggcgaccg gaagttcatc gccccggcgg
     1441 actatctgcc tgtttccgtg gcgacccttt cgacccacga ctgcgaactg gccccggaat
     1501 ggtggcgtaa cgcggacgcg gccgatcggc aggagctctg ggagttgctg ggcggg
//
LOCUS       DAOPPA010000609         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_86850_length_1555_cov_4.328000, whole genome shotgun sequence.
ACCESSION   DAOPPA010000609 DAOPPA010000000
VERSION     DAOPPA010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86850_length_1555_cov_4.328000"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1555
                     /locus_tag="PKH54_13595"
     CDS             <1..>1555
                     /locus_tag="PKH54_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD00976.1"
                     /translation="NAVQDAAPDAGPDIADPLQDAAADDAPDVANDPGDSPDATTDAD
                     AATGPDDVVDVREDAIPDADSGLEDTGFQWAGGCEPRQAYPTWTVIGDTTFKRGPLIQ
                     MSDRDTVTVVFRTENPTEDEGCVDYTWDGTPRTACGQPDVNGQYEITIEGLPAATEIH
                     YSARAGDLKTTDLTFRTMPDRPVEMKFAVFADSHANEANLKRFSELALAEGVDFVLGN
                     GDLSGEGLPEEYDLTFRGFRDLGTRVNIWTTLGNHDEKNAEGYFEAFALPQGNVDEID
                     TGYGEGWWSRRLGNVWIGGGWIRDFYLSTSDSDWGEVGWYRRQFETNEFKTAQWKLFF
                     IHQPAYSLQWDDTCVFDGDDCLKVAMIPLLSEFGVQASFHGHMHGIEYGEQNGLHMFT
                     IGGLCDCGMDMDNCQPTDIFPDPYHRIYGIPTFAIVESRCDGLKIRAVDLDGNDVMSI
                     TIPEIPIRYDENIRLMSFNLRMPFDSEPGKKWEERQQPALNIINEFNPDIMVVQEGYQ
                     FTQDFIKENI"
BASE COUNT          358 a          449 c          469 g          279 t
ORIGIN      
        1 gaatgccgtg caggacgcgg cccccgatgc cggcccggat atcgccgacc cgcttcagga
       61 cgcggcggca gacgacgcgc cggacgttgc gaatgacccc ggggattcgc cggatgccac
      121 caccgatgcc gatgcagcaa ctggccccga cgacgtggtc gatgtgcgtg aggatgcgat
      181 tccggacgcg gattccggtc tggaagacac cgggttccag tgggccgggg gatgcgagcc
      241 gcgtcaggct tacccgacat ggacggtaat tggcgacacc acattcaagc ggggaccgct
      301 gatccagatg tccgaccggg acaccgtcac cgtcgtcttc agaaccgaga atccaaccga
      361 agacgaaggc tgtgtcgatt acacgtggga cgggacgcca aggaccgcat gcggccagcc
      421 cgacgtcaac ggccagtacg aaatcaccat tgaaggactt cccgcggcga ccgaaataca
      481 ctactcggca agggcgggtg acctgaagac cacggatctg accttccgga cgatgcccga
      541 ccggcctgtg gaaatgaagt tcgccgtctt cgccgacagt catgcgaacg aggccaacct
      601 taagagattt tccgaactgg ctcttgcgga aggcgtcgat ttcgtcctcg gcaacggtga
      661 cctttccggc gaaggcctgc cggaggaata tgacctgacc ttccggggat tccgcgacct
      721 gggcacacgc gtgaacatct ggacgaccct tggaaaccac gacgaaaaga acgccgaggg
      781 atatttcgag gcatttgccc tgccgcaggg aaacgtcgat gaaatcgata ccggctacgg
      841 cgaaggctgg tggtcgcgca ggcttgggaa cgtatggatt ggcggcggat ggatccgtga
      901 tttctacctt tcgacttctg actcggactg gggagaagtc ggctggtacc gcaggcagtt
      961 tgagacaaat gaattcaaga ccgcccagtg gaaactgttc ttcattcacc agccggcgta
     1021 cagcctgcag tgggacgaca cctgcgtgtt cgacggggac gactgcctta aggtcgcaat
     1081 gattccattg ctcagcgaat tcggggttca ggcaagtttt cacggtcaca tgcacggcat
     1141 cgagtacggc gagcagaacg ggcttcacat gttcacaatc ggcggccttt gcgattgcgg
     1201 catggacatg gacaactgcc agcccaccga catctttccg gatccgtacc accggattta
     1261 cggcattccg acttttgcaa tcgtcgaatc gagatgcgat ggcctgaaga tccgggcggt
     1321 tgacctggac ggcaacgacg tgatgagcat cacaatcccg gaaattccca tccggtacga
     1381 cgaaaacatt cgcctgatga gcttcaacct gaggatgccg ttcgactcgg agcccggcaa
     1441 gaagtgggaa gaaaggcagc agccagcgct gaacatcatc aacgaattca accccgacat
     1501 catggttgtc caggagggtt accagttcac ccaggacttc atcaaggaaa acatc
//
LOCUS       DAOPPA010000610         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_87582_length_1546_cov_2.260899, whole genome shotgun sequence.
ACCESSION   DAOPPA010000610 DAOPPA010000000
VERSION     DAOPPA010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87582_length_1546_cov_2.260899"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKH54_13600"
     CDS             complement(<1..319)
                     /locus_tag="PKH54_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOD00977.1"
                     /translation="MVADDRIPFVPGRGDIVWIDLNPARGHEQAGRRPAVVVSPDDYN
                     RRVGLALMCPITSHIKGYPFEVPLPTGGCVVGAVLCDQVRSVAWRERNASLAGRLSDD
                     RIRA"
     gene            complement(313..555)
                     /locus_tag="PKH54_13605"
     CDS             complement(313..555)
                     /locus_tag="PKH54_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOD00978.1"
                     /translation="MEAKVQKWGNSLALRIPMAFARQIGLVKDGLVQIDLEGDRIFIR
                     AVSSHEFRLSDALMKVTPENMHASEDFGDPVGGEAW"
     gene            832..>1546
                     /locus_tag="PKH54_13610"
     CDS             832..>1546
                     /locus_tag="PKH54_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00979.1"
                     /translation="MKRTLTAFLLLASSGACMLSCAARESDDRTQYTVVADDNFGRKF
                     PGDKWIVGIGESSRGPADAGVQARARIAEQLESSIRTEFKSATRLDSKQSGDAAPVSS
                     ERHSITSESIVRSTFEHAELIRVVDDMGSCNGGLCTAVAVLNRDQAAAVFQREYNQAV
                     IDFQAAATQAHVLAGGGTPADAGESDIISSEGGTPVVRTDDLSAFASAYNAAARAYKS
                     IDTAAARAAAVGRPLKVAAD"
BASE COUNT          313 a          475 c          442 g          316 t
ORIGIN      
        1 ccgccctgat ccggtcatcg gaaagacgtc ctgccaagct ggcgttacgt tcccgccatg
       61 caacgcttcg aacctggtcg cagagcacgg cgccaaccac gcagcccccg gtcggcaacg
      121 gtacctcgaa cggatacccc ttgatatggc ttgtaattgg gcacatcagg gccaggccca
      181 cccgtctgtt gtagtcgtcc ggcgatacga cgaccgccgg ccgtcgcccg gcctgttcat
      241 gtccacgggc tggattcaaa tcgatccaga cgatatcacc gcgacctgga acaaacggaa
      301 tcctgtcgtc ggctaccatg cttcgccccc gaccgggtca ccaaagtctt cagacgcatg
      361 catattttca ggggttacct tcatcaaggc atctgaaagg cggaattcgt gcgaactgac
      421 agcgcgtatg aatatgcggt cgccctcgag atcaatctgc accaggccgt ccttcacaag
      481 gccgatctgc ctggcaaagg ccattggtat gcgcagcgcc agactgtttc cccacttctg
      541 aacctttgct tccatttcat gtccgcaaat gaatctacat tgaagataca atccatactc
      601 cagagtgtca aacatgaatg aatatatcgg atggttccgc ttgttgtttc aaggtatcgg
      661 cgtggatcgg tcgccttcat ccgggcactg gacggggctg ataatgaacg cttccacgac
      721 gtgtccgatt ttgctagatt acaccgtgaa aatgcacgcc gcgccgcgat tttggcaagg
      781 gtgcattcac cagtcgccat catcgggcgg aacttctgtc ggggtgactt cttgaaacgg
      841 acattgacgg ctttcctgct gctcgcttca tccggagcct gcatgctgtc ctgcgcggcg
      901 cgcgaatccg acgaccggac gcagtacacg gttgttgccg atgacaactt cgggcggaag
      961 ttccccggcg acaaatggat cgtcggtatc ggtgaatcat cccggggtcc ggccgacgct
     1021 ggcgtccagg ccagggcaag aattgccgag cagcttgaat ccagcatcag gactgaattc
     1081 aagtcggcaa cgcggctgga ttcaaagcaa tccggggatg cggcgccagt ctcttccgag
     1141 cgccattcga tcacttcgga atccatcgtg cgttcgacct ttgaacacgc cgaactgatc
     1201 cgggtcgttg atgacatggg ttcatgcaac ggcggactct gcaccgccgt ggccgtgttg
     1261 aaccgggatc aggctgcggc cgtcttccaa agggaataca accaggcggt gattgatttc
     1321 caggccgcgg cgacccaggc acatgtgctg gccggcggcg gtaccccggc ggatgcgggt
     1381 gaatccgaca tcatttccag cgaaggcggg acgcccgtcg tccggacgga tgacctttcc
     1441 gcgttcgcct cggcttacaa cgccgctgcc agggcgtaca aatcaatcga caccgccgcg
     1501 gccagggccg ctgccgtcgg gcgcccgttg aaggtcgcgg ccgaca
//
LOCUS       DAOPPA010000611         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_88312_length_1536_cov_3.714382, whole genome shotgun sequence.
ACCESSION   DAOPPA010000611 DAOPPA010000000
VERSION     DAOPPA010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88312_length_1536_cov_3.714382"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1536
                     /locus_tag="PKH54_13615"
     CDS             136..>1536
                     /locus_tag="PKH54_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00980.1"
                     /translation="MTAVPGSGFDVQLSGSFPAAFKIQTDLRHVPELDIEPGRLEISG
                     SGTVTAELGVGVAMPAMKASAISFSPRGKVGLTLAHLQVRDPQRKVKALSPITIGGTV
                     REGVIAADGDGDVGLDLEWRLETPRIEICSDRCRQVSDLPLRMMTGRQTVRVSPGGRI
                     SLAGNEGSLVRPDLLKALASPFNNAGTILELLDDDELVYGSLDSLAQLAALFNRPVAD
                     RLVAVRDWIIGVRSHARAVGVAKPGDLLDMAVLSRLVSRIIVGDESISDELRPLLSSL
                     GDGQGFDDKAARMLMIRHRPDITDTYEFDLIVSWIGILTTPVDHYNRAVPVNLPPVSD
                     DPSVAAAIRDIPAASTLYKWFSGGEPAQDKVETVVRLAPMMFIEQLEYVLSGMKGTAF
                     NQERAMLQYALSAKRAISRAVNPGELANMYIVPRASVIAGFLGDIIGPIPGIDGPGAE
                     VPPPAPLGPRDIAEILE"
BASE COUNT          272 a          460 c          471 g          333 t
ORIGIN      
        1 ggcaaccctg tttttgaact tcctgatcgg gatttcgttc ttcaatcggt cttcagtgtc
       61 ccggatcagt gtttcgggcc ggccggaaca attgacctct ttggcgcaat caggcgtggt
      121 aatctgaagg cctgcgtgac ggcggttccg ggctccgggt tcgacgtgca gctttccggc
      181 agtttcccgg cggctttcaa gattcagacc gaccttcgcc atgtgcccga actcgatatc
      241 gagccgggtc gcctggagat atccggcagc ggaaccgtga ccgccgaact gggggtcggc
      301 gtggccatgc ccgcaatgaa ggcctcggcc atttcgtttt cacctcgcgg caaggttggg
      361 ctgacgcttg cccacctgca ggttcgtgat ccccagcgca aggtaaaggc actgtccccc
      421 ataacgattg gaggtacggt ccgtgaaggc gtgatcgccg cggatggaga tggcgatgtt
      481 ggcctggatc ttgagtggcg tcttgagacg cccagaattg aaatctgttc ggaccgatgc
      541 cgccaggtgt ccgatttgcc gctgcgcatg atgaccggcc gtcagacggt gcgggtcagt
      601 ccgggcggcc gaatcagtct tgcggggaac gaagggtcgc ttgtgcgccc cgatcttctg
      661 aaggcgctgg ccagtccctt caacaatgcc gggacgattc tggaactgct cgatgacgat
      721 gaactggttt acggttcact tgattcgctg gcgcaactag cggcactgtt caaccgtccg
      781 gtggcggatc ggctcgtcgc ggttcgggac tggattatcg gggttcgaag ccacgcaagg
      841 gccgtcggcg tcgcaaagcc cggggatctg ctggacatgg cggtcctttc caggctggtt
      901 tcacgcatca tcgtcggcga cgagtcaatt tctgacgaac tgcggccttt gctgtcatcc
      961 cttggcgacg gccagggttt cgacgacaag gcggcgcgca tgctgatgat ccgtcaccgc
     1021 ccggacatca ccgacactta cgaattcgac ctgattgttt cctggatagg gattcttacg
     1081 accccggtcg atcattacaa tcgtgccgtc ccggtcaacc ttccgccggt ttcggatgat
     1141 ccttccgtgg ccgccgccat cagggacata cctgccgcat caacccttta taagtggttt
     1201 tccggtggcg agccggccca ggataaggtc gagacggtcg tccgcctggc cccgatgatg
     1261 ttcatcgagc agcttgaata cgtgctttcc gggatgaagg ggactgcttt caaccaggaa
     1321 cgcgccatgc ttcagtacgc cctgtccgcc aagagggcca tcagcagggc ggtcaacccc
     1381 ggcgaactcg cgaacatgta catcgtccca cgcgcctccg tcattgccgg attccttggc
     1441 gacataatag ggccgatccc gggtattgac ggcccggggg ctgaagtacc accaccggcg
     1501 ccgcttggcc cccgagacat cgccgaaatc ctggaa
//
LOCUS       DAOPPA010000612         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_89611_length_1520_cov_3.134471, whole genome shotgun sequence.
ACCESSION   DAOPPA010000612 DAOPPA010000000
VERSION     DAOPPA010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89611_length_1520_cov_3.134471"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PKH54_13620"
     CDS             <1..1512
                     /locus_tag="PKH54_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00981.1"
                     /translation="FTPMPDGSVEIAWLINDQHKEYFKPEPGKDRELNRPHYYPFATT
                     TAIGPGDTVFIKNTVNGEERRTFRMPLPAGQSGDTDCGEDAACLAERIRCQLDTANWN
                     LPECVKWRGWRVALPADALSAVTKRLYLGLADGDTQPVPVVCTAPDGSTPAWHVEKDG
                     DGRPIGNAICNAKDNEKAKLFGDTIEIKVFDGWVEDQATATPTETIKEFGIEVTFQGA
                     NYPVGTPLMAIATGLGYPRNTPGFRKLISMASAIIERGDSVAYVGNYAKRDKISCGCG
                     YDEGTCPDGVCRNPRANAIIYHSVGDPNVPISTGLNLGRASGALKYEGAEATPNDLLL
                     QAFVAEGNEGFRRHLSSGPNMVTYADWNGDKIIKDLRWPDEFAAEFAATPDDAMPLHA
                     DPDNQDRGLNEFGEPDVPGYTPATVQFKDNQDNVVGNIAFRIPYNYPLGAHGVEFSDP
                     RRAFNINNFVENQLALFMATGGRVLSDDPCLESGDCEFLPASIRADAAVHLKK"
BASE COUNT          346 a          464 c          460 g          250 t
ORIGIN      
        1 ttcacgccca tgccggacgg ctcggttgaa atcgcctggt tgataaacga tcagcacaag
       61 gaatacttca agccggaacc gggcaaggat cgcgagctta accgaccgca ttactatccg
      121 tttgcgacga ccaccgcaat cggacccggc gacacggtat tcatcaagaa caccgtcaac
      181 ggcgaagaac gacgcacatt caggatgcca ctgcccgcgg gacaaagcgg cgacaccgac
      241 tgcggcgagg atgcggcgtg cctggccgaa cgaatccggt gtcaacttga taccgcaaac
      301 tggaacctgc ccgaatgcgt gaagtggcgc ggatggcggg tggcgcttcc ggccgacgcg
      361 ctgtcggcgg tcaccaaaag gttgtacctg gggcttgccg atggcgacac gcagccagtt
      421 ccggttgtct gcacggcccc cgacggaagc acgccggcat ggcatgtgga aaaggatggc
      481 gacggacggc cgatcggaaa cgcgatctgc aacgcaaagg acaacgaaaa ggcgaagctg
      541 ttcggcgaca ccatcgaaat caaggttttt gatggctggg tcgaggatca ggcgacggcc
      601 acaccgaccg aaacgatcaa ggagttcggg atcgaggtta ccttccaggg cgcgaactac
      661 ccggttggaa cgcccctgat ggcaatcgcg actggacttg gatatcccag gaataccccg
      721 ggcttccgga agctgatatc gatggcgtcg gccataatcg aacgcggcga ttcggtcgcc
      781 tatgtgggca attacgcgaa acgcgacaaa atttcgtgcg gttgcgggta tgacgaaggc
      841 acatgtccgg acggcgtctg ccgcaatccc agggcgaacg ccatcattta tcattccgtc
      901 ggcgatccga acgtgccgat ttccaccggt ttgaaccttg ggcgcgccag cggggcactt
      961 aagtacgaag gcgccgaagc gacaccgaac gacctgctgc tgcaggcctt cgtggcagaa
     1021 ggcaacgaag ggttccgacg ccacctgtcg tccgggccaa acatggtcac ttacgccgac
     1081 tggaacggcg acaagatcat caaggatctg cgctggccgg acgagttcgc ggccgaattc
     1141 gcggccaccc cggacgatgc catgccgctg catgcagacc cggacaacca ggatcgcggg
     1201 ctgaacgaat tcggtgaacc ggacgtcccc ggctatacac cggcgaccgt gcagttcaag
     1261 gacaaccagg acaacgtggt tggcaacatc gcgttcagga ttccatacaa ctatccgctg
     1321 ggtgcccacg gcgtcgaatt ttccgacccg cgccgcgcgt tcaacatcaa caatttcgtg
     1381 gaaaaccagc ttgccttgtt catggcgacc ggcggtcgcg tcctgtcgga tgacccatgc
     1441 ctggaaagcg gcgactgcga attccttccc gccagcatcc gcgccgacgc cgccgtgcac
     1501 ctgaagaagt aaccaccgcc
//
LOCUS       DAOPPA010000613         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_89946_length_1516_cov_7.210815, whole genome shotgun sequence.
ACCESSION   DAOPPA010000613 DAOPPA010000000
VERSION     DAOPPA010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89946_length_1516_cov_7.210815"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1378)
                     /locus_tag="PKH54_13625"
     CDS             complement(332..1378)
                     /locus_tag="PKH54_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00982.1"
                     /translation="MKDDGRSERLSGLQPDLANYPEFQEHWERIAERIIEAGRALDIK
                     LPSVKRWIKLVEWWNNATGRRIADPSDIDTSGILGLWIGDPRNQDETVLDFLRGTIES
                     HWSGANSTIDDDEPCGDEPLDIPSDLQGWDKFEGVQIEKRRKLHDNLVAAAKAVLEMR
                     MPLDGFDYKPIPNKAGFYEVQYWEQVFPPDNACWDSLVSPEAVKAAVMLASLDNPYPA
                     PKMGRGLVSRGRTAERRALVLELNKLTRADGRPMKPKELTVFCLLVKPDYFVDVDDDG
                     IPEYLKGQGDWTPVDGSGHPNPNSLRLDAFFKSAGFVKLRDTIGKDLKPIRVGWPRTK
                     SEKSRAETPTKFQK"
BASE COUNT          274 a          458 c          410 g          374 t
ORIGIN      
        1 cttcgatttc cgattccggg aacaggcgtc gaccgccccg gcggccgacc gccgatccga
       61 ttttcaccga ggcgatgtcg cccctcaggg cgcgggtcgt caaccagtcc gtcgacacgc
      121 gcatccgcgc ggccgcttcc ttcgtcgtca gcaggtttcc cacaggtcgt gtcttcatgg
      181 ccgtttcctc cgttcgatac tctgaaaagc ggggcctttc ggccgaccgc cacccggacg
      241 acgccggcgg gccctttccg acgtcatcca cgcctcccag attaaagcct tttgtttgtt
      301 tcgcaagggg taagtatcgg taagtaaagg attatttctg gaatttagtc ggtgtttctg
      361 cgcgactttt ttccgattta gtgcggggcc agccaacgcg gatgggttta aggtccttac
      421 cgatggtgtc cctaagtttc acgaagccgg cgcttttgaa aaacgcatca agcctaaggc
      481 tgttcgggtt cgggtggccg cttccgtcaa ccggcgtcca gtccccttgc cccttcaagt
      541 attctggtat cccgtcgtca tctacgtcca cgaagtagtc cggcttgacc agcagacaaa
      601 acacagtcag ttcttttggc ttcatgggcc gtccgtcggc gcgcgtcaat ttgttcagtt
      661 ccaacaccaa tgcccgcctt tcggccgttc ggccccgtga aaccagcccc cgccccattt
      721 tcggtgcagg atatgggttg tccagggaag caagcatgac ggccgctttc accgcttccg
      781 gggaaaccaa gctgtcccag caggcgttat caggggggaa aacctgttcc caatactgga
      841 cttcataaaa cccggctttg ttcggtatcg gtttgtaatc gaagccgtcc aggggcatcc
      901 gcatttcaag aactgctttg gcggccgcaa ccaggttgtc atgtaactta cggcgttttt
      961 cgatttggac gccctcaaac ttatcccagc cttgaaggtc ggagggtata tccaggggct
     1021 catcgccaca cggctcgtca tcatcaatgg ttgaattggc ccctgaccag tgtgactcga
     1081 ttgtgcccct cagaaaatcc aagactgttt catcttggtt gcgagggtcg ccaatccaca
     1141 gcccgagaat acctgaggtg tcaatgtctg aagggtccgc gatgcgccgg cccgttgcat
     1201 tgttccacca ctcaacaagt tttatccacc gcttgactga gggtagcttt atgtccagcg
     1261 cgcggcctgc ctcaatgatc ctttccgcga ttctttccca gtgttcttga aattcaggat
     1321 agttcgcaag atcaggttgg aggccagata accgttcgct tcgtccgtcg tctttcatgc
     1381 ttcgtcgcct ccaacggccc gcgccggttc gccggccgtc gtcgattcag tcggggcctg
     1441 cattctatcg accaggaatg cgagccggcc ccggtcggtg tgtgcatatc ggcgcgacat
     1501 ggcgagggtt gcgtgt
//
LOCUS       DAOPPA010000614         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_90062_length_1515_cov_3.047260, whole genome shotgun sequence.
ACCESSION   DAOPPA010000614 DAOPPA010000000
VERSION     DAOPPA010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90062_length_1515_cov_3.047260"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /gene="carB"
                     /locus_tag="PKH54_13630"
     CDS             complement(<1..1329)
                     /gene="carB"
                     /locus_tag="PKH54_13630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD00983.1"
                     /translation="MPRDPSLKTVLVLGSGPIVIGQACEFDYSGVQAVRALKEEGIRV
                     VLLNPNPATVMTEPSTADATYIEPLRPWVVERILERENVDGVLTTLGGQTALNLAVEC
                     HDRGIWDRFGVRLLGADIRSIRMAEDRQEFRDAMESIGLPCSRGCLVTNLEDGMKAVR
                     EIGLPVILRPSFTLGGEGGGIAETEQEAAEIIRLGLKASPVGSIQVEESLIGWKEFEL
                     EVIRDRADNGIMICSIENIDPMGVHTGDSITVAPAQTLTDKELQTLRDWSLKILRRIG
                     VDCGGSNVQFALHPETGRLIVVEMNPRVSRSSALASKATGYPIARVATLLALGYTLDE
                     LPNRITKTTSAAFEPSLDYVCVKIPRWAFEKFPGLDPRLTTRMQSIGEAMALGRTFTE
                     ALGKAIRSMEKGAPRLLGAFAGLSDDRLLERLVTTSPDRLLLIAEALRRGW"
     gene            complement(1329..>1515)
                     /locus_tag="PKH54_13635"
     CDS             complement(1329..>1515)
                     /locus_tag="PKH54_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOD00984.1"
                     /translation="DRPVFSVQFHPEASPGPRDALALFDRFMELMESRSAWGGPKPRP
                     ALATDPEITGTDGRRSM"
BASE COUNT          285 a          486 c          455 g          289 t
ORIGIN      
        1 ccatcctcgg cgcagggcct cggcaatcaa cagaaggcgg tcaggcgatg tcgtgacaag
       61 cctttccaga agcctgtcgt cggaaaggcc ggcaaacgcc ccaagcagcc gcggggcccc
      121 cttttccatg ctgcggatcg ccttgccaag ggcttccgtg aacgtgcggc caagcgccat
      181 ggcctcgcct atcgactgca tccttgtcgt cagtctggga tcaagtccgg gaaacttttc
      241 gaacgcccac cgtggaatct tcacgcatac gtagtcaagc gacggctcga acgccgccga
      301 cgttgtcttc gtgatgcggt tgggaagttc gtcaagcgta tatccaaggg caagcagcgt
      361 ggcgacccgc gcaatcgggt aaccggtggc cttcgaagcc agggccgacg accggctgac
      421 ccgtggattc atttcgacga ctatcaggcg tccggtctcc ggatgaagcg cgaactgcac
      481 gttcgagccg ccgcaatcga ccccgatccg gcgcagaatt ttcaaagacc agtcgcgcag
      541 ggtttgaagt tccttgtcgg taagcgtctg ggccggcgcg accgttatgg aatccccggt
      601 atggacgccc atcgggtcga tgttttcaat cgagcagatc atgatgccgt tgtcggcacg
      661 gtcgcgaatg acctcaagct cgaattcctt ccagccgatc agggattcct cgacctggat
      721 cgaaccgacc gggctggcct tcaggcccag ccggatgatt tcggcagcct cctgttcggt
      781 ttccgcgatg ccgccgcctt ccccgccaag cgtgaaggac gggcgaagga ttactggcag
      841 cccgatttca cgaaccgcct tcatcccgtc ttcaaggttt gtcaccaggc aaccgcggga
      901 gcagggaagg ccgattgatt ccatggcgtc gcggaattcc tgacgatcct ccgccatccg
      961 tatcgacctg atgtcggccc ccagcagcct gacaccgaac ctgtcccaga ttccgcggtc
     1021 atgacattcg accgccaggt tcagcgcggt ctggccgccg agcgtggtca gcacgccatc
     1081 gacgttctcg cgttcaagta tgcgttcgac gacccacggg cgcagcggtt cgatgtacgt
     1141 cgcgtccgcc gtcgatggtt ccgtcatgac cgtcgccggg ttagggttca gcagaacgac
     1201 ccggatccct tcttccttca gggctcgcac cgcctggacg cccgagtagt cgaactcgca
     1261 cgcctggccg atgacgatcg gtccggaccc aagaaccagc accgttttca acgaaggatc
     1321 gcgtggcatt acattgacct ccgtccgtca gtgccggtga tttccggatc ggtagcaagc
     1381 gcgggcctgg gcttcggacc accccaggcg gatcgggact ccatcagttc catgaatcga
     1441 tcgaaaagag cgagcgcgtc cctggggccg gggctggcct cgggatggaa ctggaccgaa
     1501 aagacggggc gatcc
//
LOCUS       DAOPPA010000615         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_90304_length_1512_cov_3.230611, whole genome shotgun sequence.
ACCESSION   DAOPPA010000615 DAOPPA010000000
VERSION     DAOPPA010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90304_length_1512_cov_3.230611"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>1512)
                     /locus_tag="PKH54_13640"
     CDS             complement(70..>1512)
                     /locus_tag="PKH54_13640"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HOD00985.1"
                     /translation="NDGFAKKWHDAVLNYALPWDPWWTTAVHESGDPWCGIHQRLHGQ
                     QRDAITEALHAPYYTDDVALPVDPSAWVDKINVPVFMTGQWQDEQTGPHFAALMDKFK
                     GSPNVHFAVTNGFHNDSAAPQDLIEWMEFLYLFVAREIPRPDEGVLGLGGIFMDKIFG
                     AQIDIKQTPLAEAATYEEALAMWDARPPVRVIFESGTNPKEDPAAPVGAFEQRFDAWP
                     IPGTVVDRWTLGLNSTMTQAAPADAAGAASNADTDGTGEPWELYTGFVADAEAGHRVT
                     LASGEVYDDLPPDWSWRQPQSGNAVDFITPPLTEDRVMLGSGSVDLWIRSNHDDADVE
                     VTLTEVRPDGMETYVQVGWLRLSQRALREDATELRPVKTHREADLQPLVPGEWTFARV
                     EIMPFGHVFRAGSRIRLIVDTPGDSMASWMFNLKNWGDPNVIEIAVDENHQSSLALPL
                     VPGVTVPTDLATDCTALRGQPCRPFQAIVP"
BASE COUNT          237 a          452 c          535 g          288 t
ORIGIN      
        1 gcggagtgat ttcctgtttc aagactgact gttatgcggc ggatggtcag ggcgccgggt
       61 tctgcccggt cacggcacga tggcctggaa ggggcggcag ggctggccac gcagggcggt
      121 gcagtcagtc gcaaggtcgg tcgggacggt cacgccgggg accaggggca gggccaggct
      181 ggactgatga ttttcatcga ccgcaatctc gatgacgttc gggtcgcccc agttcttcag
      241 gttgaacatc catgaagcca tgctgtcgcc gggggtatcg acgatcaggc ggatgcgcga
      301 gccggcgcgg aacacgtggc cgaacggcat gatttccacg cgggcgaacg tccattcacc
      361 ggggaccagc ggctgcaggt cggcctcgcg gtgggtcttg acggggcgca gttcggtcgc
      421 gtcttcgcga agggctcgct gggacaggcg cagccagccg acttggacgt aagtttccat
      481 cccgtcgggg cggacttcag tcagtgtgac ttcaacgtcg gcgtcgtcgt ggttgctgcg
      541 gatccacagg tcaacgctgc ctgatcccag catcacgcga tcctcggtca ggggcggcgt
      601 gatgaaatcg acggcgtttc cggattgcgg ctgacgccag gaccagtcgg gcggcaggtc
      661 gtcatagact tcgccggacg ccagggtgac tcggtggccg gcttccgcgt cggcaacgaa
      721 gccggtgtaa agctcccagg gctcgcccgt cccgtcggtg tcggcgttgg atgcggcgcc
      781 tgcggcgtcc gcgggcgcgg cctgggtcat cgtcgagttc aggccaagcg tccaccgatc
      841 gaccactgtt cccgggatcg gccatgcgtc gaagcgctgc tcgaaagcgc cgaccggcgc
      901 ggcaggatcc tccttcgggt tcgtcccgga ttcaaagatg acgcgcacgg gcggccgcgc
      961 gtcccacatg gccagggctt cttcatatgt ggcggcttcg gccagcggtg tctgcttgat
     1021 gtcgatctgg gccccgaaaa tcttgtccat gaagatgccg ccaaggccca ggacgccttc
     1081 gtccgggcgc gggatttccc gggccacgaa caggtacagg aactccatcc attcaatcag
     1141 gtcctggggc gccgcgctgt cgttgtggaa gccgttggtg acagcgaagt ggacgttggg
     1201 cgagcccttg aatttgtcca tcagggccgc gaagtgcggg ccggtctgtt cgtcctgcca
     1261 ctggccggtc atgaacaccg ggacgttgat tttgtccacc caggccgacg gatcgaccgg
     1321 gagcgcgaca tcgtccgtgt agtagggggc atgcagtgct tccgttatgg cgtcgcgctg
     1381 ctggccgtgc aggcgctggt ggattccgca ccacgggtcg cccgattcgt ggacggcggt
     1441 ggtccaccag ggatcccatg gaagcgcgta gttaaggacg gcgtcgtgcc actttttcgc
     1501 gaaaccatcg tt
//
LOCUS       DAOPPA010000616         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_91126_length_1502_cov_3.871458, whole genome shotgun sequence.
ACCESSION   DAOPPA010000616 DAOPPA010000000
VERSION     DAOPPA010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91126_length_1502_cov_3.871458"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKH54_13645"
     CDS             complement(<1..1115)
                     /locus_tag="PKH54_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOD00986.1"
                     /translation="MTLNSIQEIIAEGTTSDEKKLGLISSIAVVVGNMVGIGIFLTPA
                     EVARASPDMLTFVGIWLVGTLMAIAGAQVYAELGVLFPKAGGDYRFLFEAYGPRPARA
                     WGWMSVFGCFPASIATLAVGVTVTLSETSMGFFLNNQLLSFHGIHITPATFVGLALIW
                     ISTAVNVSGLSISGRTQLLVTLVPILTFVVAGIFATGSSFGGQASQPNNVVPNISLVT
                     TALCAVFFTFSGWNVITYLGGEIHRPKLNIPRVIVAAISITTVIFLILNLSFLSVIPL
                     PRLAATQNAGVAVATSIFGDPGRDIFGLLLAMAIVAGLNATVMAGSRISVAMAETNQV
                     WRGLAHRSGARETPQRALVMQAVIASVLVLSGSFTML"
     gene            1279..>1502
                     /locus_tag="PKH54_13650"
     CDS             1279..>1502
                     /locus_tag="PKH54_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00987.1"
                     /translation="MSVKMAGTIVCLLSVLGVAVPALAADSESDASAKPVVAVFKFKQ
                     NQITLKQDQVDFLFQQLRDQLLGQGVFQVAP"
BASE COUNT          330 a          424 c          458 g          290 t
ORIGIN      
        1 atcagcatcg tgaaacttcc ggaaagcacc aggacagacg cgattacggc ctgcatcaca
       61 agtgctcgct ggggtgtctc cctggccccc gaacggtgcg ccagccccct ccagacctga
      121 ttcgtttcgg ccatggccac cgaaattctg gaaccggcca taacggttgc gttgaggccc
      181 gcgacgatag ccatcgccag cagcaggccg aagatgtccc ttccaggatc gccgaagatc
      241 gacgtggcga cggcgacccc cgcgttctgg gtcgccgcga gcctgggaag cggaatcacg
      301 gaaagaaacg acaggttcag gatcaggaag atgacggtcg taatcgaaat cgccgcgacg
      361 atgacccgcg gtatgttcag cttcgggcgg tggatttccc cgccaagata ggtgatcacg
      421 ttccagcccg agaacgtgaa gaaaacggcg cacaacgccg tggtcaccag cgagatgttc
      481 ggaaccacgt tgtttggttg cgatgcctgg ccgccaaagg aactgccggt cgcgaaaatg
      541 ccggcaacca cgaaggtgag gatcgggaca agcgttacca ggagttgcgt ccggccgctg
      601 attgaaaggc cggacacgtt gaccgccgtg gatatccaga tgagtgccag gccgacgaag
      661 gtcgccggcg tgatgtggat gccgtgaaac gaaagaagct ggttgttcag gaagaacccc
      721 atcgaggttt cggaaagggt gactgtgacg ccgaccgcga gcgtcgcgat ggacgcgggg
      781 aagcatccga atacagacat ccagccccag gccctggccg gccttggacc atacgcttcg
      841 aacagaaatc ggtagtcgcc gccggctttc gggaacagaa cgccgagttc ggcatagacc
      901 tgggcgcccg cgattgccat cagcgttccc accagccaga tcccgacgaa agtcagcatg
      961 tccggcgaag ccctggcaac ctcggccgga gtaaggaata tgccgattcc gaccatgtta
     1021 cctacgacca cggcgatcga tgaaatcagg cccagctttt tttcatcgct ggtcgttccc
     1081 tcggcgatga tttcctgaat tgaattcagc gtcattgatg cagcgcaatt cctgacgttg
     1141 cgatcataaa tccgttgtgc gggaatgcta agaaaacggc agtgtcgcca cgtcagcggc
     1201 ttgccggatc gtcgtcatcg tgacggcgtt gctgcaatcg tgtaacattg tcatctgatt
     1261 gcgaaacgag gtgtcgatat gtccgtgaag atggccggaa caatcgtttg cttattgtcc
     1321 gttcttggtg tggcggtccc ggcacttgcg gccgattcgg aatcggatgc gtccgcaaag
     1381 ccggtggtcg ccgtgttcaa gttcaagcag aaccagataa ccctgaagca ggatcaggtg
     1441 gattttcttt tccagcagct tcgtgaccag cttctggggc agggcgtgtt ccaggtcgcg
     1501 cc
//
LOCUS       DAOPPA010000617         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_91380_length_1499_cov_9.049861, whole genome shotgun sequence.
ACCESSION   DAOPPA010000617 DAOPPA010000000
VERSION     DAOPPA010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91380_length_1499_cov_9.049861"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKH54_13655"
     CDS             <1..507
                     /locus_tag="PKH54_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00988.1"
                     /translation="PFGLLPDRGISCFVDPGARVVQHLIDHHRDRIELVPVGASSALD
                     AAIMMSGVDCTFFSFMGHFPEQYRWPVDSVCAGIPVVSYVRFDSAARFWQAARSTLGQ
                     PSGLRISFFRNIRGRQRRFQGNFPLDMDPATTGFPGFDPRDLEGTGSDNFVAMIHPPW
                     FVEDSGRG"
     gene            512..>1499
                     /locus_tag="PKH54_13660"
     CDS             512..>1499
                     /locus_tag="PKH54_13660"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOD00989.1"
                     /translation="MTPLDRGHGMETSGMQDSFGFRLLNSCPPTGARTGLMKTAHGDV
                     KTPCVAPIRFFGNGVAMSTDELTRAGVEIVSDRLDSLAQTPALDSIAALGGIHRFSGW
                     QRPIIARFDPNGLRAATDRFAQDADGLSFWSHRFGRRIQFDPVDMTRRVQELGFDIGL
                     GLLPADAEDPRDHGLCRDIALRWAIDGMRARTRTDMGLLGPAWLEQDKASGEAISPVD
                     ARRFDGLWADSRGEPGNDHHSIDGIRMATDSDRPDHLWKLAAAGFDVIEVSWPVRYAR
                     LGLLVTSSGTVNLKDGANDLIADPPDKQCGCPVCSTCSRALLRFMHMSGEPVS"
BASE COUNT          268 a          468 c          459 g          304 t
ORIGIN      
        1 ccgttcggat tgctgcctga ccgcggaatc agctgcttcg tcgaccccgg ggccagggtc
       61 gtccagcacc ttatcgacca ccaccgcgac cgcatcgaac tggttccagt gggagcctca
      121 tccgcgctgg acgccgccat catgatgtcc ggcgtcgact gcaccttctt ttcattcatg
      181 ggacacttcc ccgagcagta tcgctggcct gttgattcgg tctgcgcggg gatacccgtt
      241 gtctcttacg tccgtttcga ttctgccgca cgcttctggc aggccgccag atcgacgctc
      301 ggtcagccga gcggactgcg aatctccttc ttcaggaata tccgtggcag gcagcgccgc
      361 ttccagggaa actttcctct ggacatggac cctgcgacga ccggctttcc cggctttgat
      421 ccgcgggatc tggaaggaac cggctccgac aacttcgtgg caatgatcca cccgccctgg
      481 ttcgtcgagg actcggggcg aggatgactc cgtgaccccc cttgaccggg gacacgggat
      541 ggagacttcc gggatgcagg actcgttcgg cttcaggttg ttgaattcat gcccgcccac
      601 cggcgcccgc actggactga tgaaaacagc ccacggtgac gtcaagaccc cgtgtgtcgc
      661 tccaatccgg tttttcggca acggcgtggc gatgtccacg gacgaattga ccagggccgg
      721 cgttgaaatc gtatctgacc gactcgactc cctcgcgcag acgccggcgc tcgattcaat
      781 cgccgcgctc ggaggaatcc accggttcag tggctggcag cgcccgatca tcgccaggtt
      841 cgacccgaac gggctgcggg cggcgaccga ccgcttcgcg caggacgcgg atggattgtc
      901 gttctggtcg cacaggttcg gacggcgtat tcaattcgat ccggtcgaca tgacgcggcg
      961 tgttcaagag ctgggattcg atattggtct tggacttctg ccggcagacg ctgaagatcc
     1021 ccgggaccac ggactctgcc gcgacatcgc gctccgatgg gcgattgacg gcatgcgggc
     1081 acgcacgcga accgacatgg gtctgcttgg tccggcgtgg cttgaacagg acaaagccag
     1141 tggtgaggca atctcccccg tcgatgcgcg tcggttcgac ggactctggg ccgacagcag
     1201 gggcgaacct ggaaatgatc accattcaat cgacgggatt cgaatggcga ccgactcgga
     1261 caggcccgac catctctgga aacttgccgc ggcgggattc gatgtgatcg aggtctcctg
     1321 gccggtcaga tacgcaagac tgggcttgct cgttacgtct tcgggaacag tgaatctgaa
     1381 agatggcgcc aacgatctga ttgccgatcc gccggacaaa cagtgtggat gtccggtctg
     1441 ctcaacgtgc tcccgcgctc tgctgcgatt catgcatatg tccggcgaac cggtttcaa
//
LOCUS       DAOPPA010000618         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_92065_length_1492_cov_2.610995, whole genome shotgun sequence.
ACCESSION   DAOPPA010000618 DAOPPA010000000
VERSION     DAOPPA010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92065_length_1492_cov_2.610995"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1492
                     /locus_tag="PKH54_13665"
     CDS             <1..>1492
                     /locus_tag="PKH54_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00990.1"
                     /translation="AMVRGSTGMTVNAIGYVFVAGKTDVTNGNDNPAGLVGQYGYGPA
                     DSIPGNDWTWDNASPTASNLSGRDDADSYRYSFVVPVADEGHYDMAFRFSADGGYSWK
                     YCDLDGTDDGYDVADAGKLEIVSDPIVLITEYVDGTGNDKAIELLNLSGRDLNLKNCA
                     ISIYTDGAVSPSTIFNVEDGVLQDAASYVMCQTGVSTDLTPYCDEFRSLLNFNGNDTI
                     SLTCEDKLMDSFGRRGENPGASGWGTGEVTSVGHTLRRHCDVIFGDTDLSNEFDPGLE
                     WWSFPRDSFLDLGSNLCAQACHVGGRETCCDNVNDDDLDGLQDCMDPDCDEAVECLPP
                     VIGYAAVMMPGTGAWVFAEIPEVLFGRVFVQGITNVAGQGLGVMAQGGWGPDGSNPDG
                     HILWSWYDAVYFDDIDGSDDRYGVSFDDVPVGTFDYAFRFSTDGGDTWTYADLEPAGT
                     IDGYSAATTGSIIVTAGVETACDDHADNDGDLQTDCNDPDCATDPY"
BASE COUNT          302 a          452 c          461 g          277 t
ORIGIN      
        1 cggccatggt tcgcggttcc accggaatga cggtcaacgc catcgggtac gtcttcgtcg
       61 ctggcaagac cgatgtcacg aacggcaacg acaacccggc cggcctggtt ggacagtacg
      121 gctacggccc ggcggactcg attcccggca acgactggac atgggacaac gcttctccga
      181 ccgcaagcaa cctgagcgga agggacgacg ccgacagcta ccgttattca ttcgtggtgc
      241 cggtcgcgga tgaaggccat tacgatatgg ctttcaggtt ctcggccgac ggcggatatt
      301 cctggaagta ctgcgatctt gacggaaccg acgacggata cgacgtggcg gacgccggca
      361 agctggaaat cgtgtcggat ccgatcgtgc tcatcaccga atacgttgac ggcaccggga
      421 acgacaaggc gatcgagttg ctgaacctga gcggccgtga cctcaacctt aagaactgcg
      481 cgatatccat ttacaccgac ggggcggtca gcccatcgac catcttcaat gtcgaggacg
      541 gcgttcttca ggatgcggcg tcctatgtga tgtgccagac cggggtctcg accgacctta
      601 ccccttactg tgacgaattc agatccctct tgaatttcaa cggcaacgac acaatcagcc
      661 tgacgtgcga agacaagctg atggattcgt tcggcaggcg cggggaaaac ccgggcgcct
      721 cgggatgggg aacgggcgaa gtcaccagcg tcggacacac cctgcgccgt cattgtgatg
      781 taatcttcgg ggacaccgac ctttcgaacg agttcgatcc cgggctcgaa tggtggtctt
      841 tcccgcggga ctccttcctg gatcttggtt caaacctctg cgcccaggca tgccatgtgg
      901 gtggcaggga aacctgttgc gacaacgtca acgacgacga cctggacggc cttcaggact
      961 gcatggatcc cgactgcgac gaggcggtcg aatgcctgcc gccggtaatc gggtacgcgg
     1021 ccgtcatgat gcccggcacc ggcgcctggg tcttcgccga gatccctgaa gtacttttcg
     1081 gcagggtatt cgtgcagggc atcaccaacg tcgcgggcca gggcctggga gtgatggccc
     1141 agggtggatg gggccctgac ggcagcaacc ccgacggaca tatcctgtgg tcctggtacg
     1201 acgcggtcta tttcgacgac attgacggtt ctgacgacag atacggggtt tccttcgacg
     1261 acgttccggt cggaaccttc gactacgcat tccgtttcag caccgacggc ggcgacacgt
     1321 ggacttacgc ggatcttgaa ccggctggca ccatcgacgg ctactcggcg gcgaccaccg
     1381 gttcgatcat tgtgaccgcc ggggtcgaaa ccgcatgcga cgaccacgcg gacaacgacg
     1441 gcgacctcca gaccgactgc aacgacccgg actgcgcgac tgatccgtac tg
//
LOCUS       DAOPPA010000619         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_92761_length_1484_cov_3.415675, whole genome shotgun sequence.
ACCESSION   DAOPPA010000619 DAOPPA010000000
VERSION     DAOPPA010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92761_length_1484_cov_3.415675"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKH54_13670"
     CDS             <1..226
                     /locus_tag="PKH54_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00991.1"
                     /translation="GNGGDGGDGGGGPGGPTFPLYIVGASSNPTCETNEYMLVGLGGD
                     GGFSGGSGSTKGQTGESGHIFGITSKCSLQ"
     gene            314..>1484
                     /locus_tag="PKH54_13675"
     CDS             314..>1484
                     /locus_tag="PKH54_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOD00992.1"
                     /translation="MKLYLIDASGLVHRAFHAIRGLTNSQGMPTNAIFGLATMLNRFL
                     AQERPTHCAFVQDVGRETFRNRLYQGYKANRPETDPALSVQFKYVPRLASALGFPVVA
                     VPDFEADDVIATLARQAVDAGVDVVIESGDKDLLQLVGPGVTVHDPMKEKYYDREAVF
                     EKLGVHPEFVRDYLALVGDSSDNVPGVHGIGARGAADLINSFGRLEDILKSIDSVPPR
                     ARKALEQYADDARLSMKLVTLEDMAPVGVSIGELAMGQPDSGALAGLYSELGFRKLLA
                     ELHGAEGTPRVDAGSSTSQGTGPAGPERPAFVEQDLFDAPEADRIQLSADISPKVVEP
                     WRISGILQLLSSVHKEAFFTPWPEASAGFLTDDNQCYIISLSDAARLAPDRLAGAF"
BASE COUNT          292 a          451 c          470 g          271 t
ORIGIN      
        1 cggcaacggc ggcgacggtg gcgacggtgg cggcggaccg ggcggcccga cgttcccgct
       61 ttacatagtc ggtgcatcct cgaacccgac atgcgaaacc aacgaataca tgcttgtcgg
      121 ccttggcggc gacggagggt tcagcggtgg cagcggctcg accaagggcc agaccggcga
      181 gtcgggacac atcttcggca tcacgtccaa atgcagcctg cagtaacgcc acaatctcac
      241 catacgcaac catgctcccc aaaagctcga tgttccgtgc cataatcggc gtgcaagtct
      301 cccgggaatc gaaatgaagc tttatctgat tgacgcaagc ggccttgttc acagggcttt
      361 ccacgcaatc cgcggcctga cgaacagcca ggggatgccg accaacgcca tcttcgggct
      421 cgcgacgatg ctgaacaggt ttctggcaca ggaaaggccg acgcactgcg cattcgtgca
      481 agacgttgga agggagactt tcaggaacag gctgtatcag ggatacaagg caaaccggcc
      541 ggaaacggat ccggcccttt cagttcagtt caagtatgtt ccgaggctcg catcggcgct
      601 gggctttccc gttgtcgcag ttccggactt cgaggccgac gacgtcatcg ccactctcgc
      661 caggcaggcg gtcgatgccg gcgtcgatgt ggtgatcgag tccggcgaca aggatctcct
      721 gcaactggtc gggccgggcg tgacggttca cgaccccatg aaggagaagt actacgaccg
      781 cgaagcggtt tttgagaagc tcggggttca tcccgaattc gtccgggatt acctggccct
      841 ggtgggggat tcgtccgaca acgtgccggg ggtgcacggg attggcgcgc gcggcgcggc
      901 agacctgatc aactcgttcg gccgccttga ggacatcctg aagtcgattg actcggttcc
      961 gccacgcgcg cggaaggccc tggagcagta cgccgatgat gccaggctgt ccatgaagct
     1021 ggtgaccctg gaagacatgg cgccggtcgg ggtttcaatc ggtgaactgg ccatgggcca
     1081 gcccgacagc ggggcgcttg ccggacttta ttccgaactt gggttcagga agctgcttgc
     1141 ggagttgcac ggggccgaag ggacgccccg ggttgatgcc ggcagttcga catcacaggg
     1201 aaccggaccg gcaggccccg aacgtcccgc cttcgtcgag caggaccttt tcgatgcgcc
     1261 ggaggccgac cggatccaac tctcggccga catcagcccc aaggtggtgg agccctggcg
     1321 aatctcggga atccttcaac tcctgtcgtc agttcacaag gaagcctttt tcacgccctg
     1381 gcccgaggca tccgcaggat tcctaaccga cgacaaccag tgttacatca tcagtctttc
     1441 cgatgcggca agactcgcgc ccgacaggct ggctggggct ttcc
//
LOCUS       DAOPPA010000620         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_93295_length_1478_cov_2.996486, whole genome shotgun sequence.
ACCESSION   DAOPPA010000620 DAOPPA010000000
VERSION     DAOPPA010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93295_length_1478_cov_2.996486"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="murJ"
                     /locus_tag="PKH54_13680"
     CDS             <1..1377
                     /gene="murJ"
                     /locus_tag="PKH54_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOD00993.1"
                     /translation="RLTRFYSGFMTLFILLMLTITLLGIAFAEPIVRYIFASKFADVP
                     GKMELTIGLTRLMFPYLMIISVAAIVQATLNAFKIFGPSAFTPVLLSIAIIAAVALFH
                     DSFPNPAWALAIGFVVGGIFQLLFQIPFLRKAGVRFRPTLAGLKDSSVRLVGRQFLPG
                     ILGAGVYQINIAVSQIIATTLAPGTVASLHYSLRLQEVVLGIFAVSVSQVILPTMSEQ
                     STLGDRKGLGDTLEFSVRLMALVTIPATAGLIILGDPIIRLLFQYGRFGEESVRMTVW
                     ALNFHAAGIFFIALHRNATQVFYAMKDMKTPVIVAVVSMIVNVGLCLALAGPMQNGGI
                     ALAGSVASLASAALTLVILHVRGGFFNPGRMFGSLPKVLAATGAMAALLLVLRFYFGF
                     FDIQGYGGLGMFERVRQMGPRVAISIPAAMLVFVVAARFLHVREVGEISAMVKRRINR
                     RKAGRDRI"
BASE COUNT          236 a          422 c          447 g          373 t
ORIGIN      
        1 cgcctgaccc ggttctattc cggcttcatg acccttttca tcctgttgat gcttaccatc
       61 acgttgcttg ggattgcctt cgccgagccg attgtcaggt acatcttcgc ctcgaaattc
      121 gctgatgtcc ccggaaagat ggagcttaca atcggcctga ccaggttgat gtttccgtac
      181 ctgatgatca tttcggtggc cgcgattgtc caggcgacct tgaacgcgtt caagattttc
      241 gggccgtccg ccttcacgcc ggttcttctt tcaatcgcca taatcgcggc cgtcgcgctt
      301 ttccacgatt ccttcccgaa tccggcctgg gcgctggcca tcggatttgt cgtcggcggg
      361 atttttcagc tcctgttcca gattcccttt ctgcggaagg ctggcgtgcg gttcaggccg
      421 actctggccg gtttgaagga cagttcggta aggcttgtcg ggcgccagtt tcttcccggc
      481 atactggggg ccggcgtata tcagatcaac atcgcggttt cccagataat cgcgaccacg
      541 ctggcgccgg gcacggttgc aagtcttcat tattccctgc ggttgcagga agtggtgctc
      601 gggattttcg cggtgtcggt ttcccaggtg atcctgccca ccatgtccga gcaatcgacg
      661 ctgggcgacc gcaagggact tggggacacg ctggagtttt cggtaaggct gatggcgctc
      721 gtaacgattc cggcaacggc cggcttgata atcctgggcg atccgattat ccgcctgctc
      781 ttccagtatg gccggttcgg cgaggaatcg gttcggatga cggtctgggc gctgaatttt
      841 catgccgccg gaatcttctt cattgccctt catcggaacg cgacccaggt cttttatgcc
      901 atgaaggata tgaagacacc ggtcatcgtc gcggtggttt cgatgatcgt caacgtcggc
      961 ttgtgcctcg cgctggccgg cccgatgcag aacggtggaa tcgcccttgc gggatccgtg
     1021 gcgtcgcttg cgagtgccgc cttgacgctg gtcatactgc atgttcgggg cggtttcttc
     1081 aatcctggcc gcatgttcgg ttcacttcca aaggtactgg ccgccaccgg cgcgatggcg
     1141 gcattgctgc ttgtgcttcg tttttacttt ggtttcttcg atatccaggg gtacggcggg
     1201 ctggggatgt tcgaaagggt gcgacagatg ggcccccggg tagcgatctc aattccggcg
     1261 gcgatgcttg tctttgtcgt ggctgcccgt tttctgcatg ttcgcgaagt cggcgaaatc
     1321 tcggcgatgg tgaagcgacg catcaatcgg cgcaaggccg ggcgggacag gatctagcgg
     1381 gcgcacctga acccgatttc accgtcaagt cccgaatcga tcacgatcaa ttcgcggttg
     1441 accgaacgca gatcgaccga cgcggcgccg cccagggg
//
LOCUS       DAOPPA010000621         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_93379_length_1477_cov_3.271449, whole genome shotgun sequence.
ACCESSION   DAOPPA010000621 DAOPPA010000000
VERSION     DAOPPA010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93379_length_1477_cov_3.271449"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PKH54_13685"
     CDS             <1..1369
                     /locus_tag="PKH54_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD00994.1"
                     /translation="TGEPMPNPAEDVICGAAPEQRRADLASLISRGNRTCLIESSASV
                     VGGSGDTCQGTVIVFRDVTQKQLFQDQIQHSRKMEAIGQLAGGVAHDFNNMLAAIQGY
                     AELLNRRAAPDSPEQKFCQNIFNATERAAGLTSKLLAFARKGKALSTPIDVHESIRNA
                     LGLLERSLDKSIVITSRLDAQNSMVVGDPSQIQSAILNLCINARDAMPNGGRLEVFTD
                     NLVLSEIDCRSSEFKILPGEYVGIHVRDTGTGIDPKTLSHIFEPFFTTKEVGRGTGLG
                     LAAVHGAIVEHGGAIKVYTEIGRGTQFNIYLPVSHDDAYRPPEEYRTTIPHEGLVLIV
                     EDESIISSMAETMLLEAGFEVVTAENGRIGVQKFRENLDRLRLVIIDVIMPEMDGMTA
                     LKAMREISPTMPFIVASGFSFDHRRDEFIRAGARSFLAKPFRNSELIKAVDDCLMYSE
                     QIPRQ"
BASE COUNT          345 a          439 c          425 g          268 t
ORIGIN      
        1 cacgggcgaa ccgatgccga atccggccga ggacgtcatc tgcggggcgg cgccggagca
       61 aaggcgcgcc gatctggcaa gcctcatttc aagaggcaac cgaacctgcc ttatcgaaag
      121 cagcgcttcc gtggtcggtg gaagcggcga tacatgccag ggcaccgtca tcgtcttccg
      181 cgacgtgacg cagaagcagc tcttccagga ccagatccag cattccagga agatggaggc
      241 catcggtcag cttgccggcg gagttgcaca cgacttcaac aacatgcttg cggccatcca
      301 gggatacgcc gaactgttga accgtcgcgc ggcgccggac agtccggaac agaagttctg
      361 ccagaacatc ttcaacgcga ccgagcgtgc cgccggactg acatcgaagc ttctggcttt
      421 cgcccggaag ggaaaggctc tctcgacgcc cattgacgtg cacgaatcaa tccgcaacgc
      481 actcggcctt cttgaacgaa gcctggacaa atccatagtc atcacatcga gacttgatgc
      541 gcaaaacagc atggtcgtgg gcgatccttc ccagatacag tcggccatac tcaacctgtg
      601 catcaacgct cgcgatgcga tgccgaatgg cggacgcctt gaagtgttca ccgacaacct
      661 ggtgctttcc gaaatcgact gccggtcaag cgaattcaag atcctgccgg gtgagtatgt
      721 cgggatacac gtcagggaca caggcactgg gatagatccc aaaacccttt cacacatctt
      781 cgaaccgttc ttcaccacca aggaagtcgg tcggggaacc ggactcgggc tggcggccgt
      841 gcacggtgca atcgtcgagc acggtggcgc gataaaggtc tataccgaga tcggccgtgg
      901 aacccagttc aacatctatc tgccggtcag ccacgatgac gcatacagac caccggaaga
      961 gtatcgcacg accattccac acgaaggcct ggtgctgatt gtcgaggatg agtcgataat
     1021 cagttcaatg gccgaaacca tgctgcttga ggcgggcttc gaggtggtga ctgccgaaaa
     1081 cgggcggatc ggagtccaga aattccgtga aaacctcgat cggcttaggc tggtaatcat
     1141 tgacgtgatc atgcccgaga tggacggaat gactgcattg aaggcgatgc gggaaatcag
     1201 cccgacaatg ccgttcatcg tcgcttccgg tttctcattc gaccacaggc gggacgaatt
     1261 cataagggcc ggcgcaagat ccttccttgc gaaaccattc agaaattcgg aattgatcaa
     1321 ggctgttgac gactgcctta tgtactccga gcagattccg aggcaatgat cgcgaccgct
     1381 tcggaccaga gcgaaagcgc gcgtgcccgg ccctggaaga tggcccccct gccgcctccc
     1441 ctcccctgga ggatcccggc aacggccggg ccgacgc
//
LOCUS       DAOPPA010000622         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_93566_length_1475_cov_3.754225, whole genome shotgun sequence.
ACCESSION   DAOPPA010000622 DAOPPA010000000
VERSION     DAOPPA010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93566_length_1475_cov_3.754225"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1475
                     /locus_tag="PKH54_13690"
     CDS             <1..>1475
                     /locus_tag="PKH54_13690"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOD00995.1"
                     /translation="YNRFGGPARLGIFHDFHPQASGEGAFTWSDAFARLRLGKVMIPD
                     DERPAPEPDNPNPFAAVWPFMPADADGLDAESFCTVVESLAARLEVLRTPFMPVADWG
                     MALTSLFDDFLSVPADRKPEELVRQSLVSGLQFYFGGRSILDSSLKTVGASEENRLIP
                     AHLAFEIVRGLAGSIPTGRGAYLSEGVTVATLKPMRPIPFKVTFILGLEEGGFPGAAR
                     ESSLDLRGGDRRIGDVTTPDSGRHMFMETLLSTTQSLVLSYDAWDTMKDERHQPCSVV
                     RQLIDFVEANVLPGQGDDGGFKELEIPLSPWGERYLGADAPVPQGATRIADRLVAGLL
                     GAAGSARAPGLAAAIEGAVGNVAVSGRDRQLLRAALDLVSDHRDCRRADGSAMAEPGD
                     SSTPEYDSGPITVTIQELNRFLRDPLKARLARFFGRQDDLDKPDPSGLEDEGFEFETF
                     TGRTLKENWLKDILGGMDPDRAWARLENSGRALSMRSSAPSP"
BASE COUNT          252 a          435 c          488 g          300 t
ORIGIN      
        1 tacaaccgat ttggagggcc tgctcgactt gggatcttcc acgattttca tccacaggcg
       61 tccggggagg gcgcgttcac ctggtcggac gcattcgcca ggttgcgcct tggcaaggtg
      121 atgatcccgg acgatgaacg tccggccccg gaaccggaca atccgaatcc ctttgctgct
      181 gtatggccgt tcatgccggc ggacgcggac gggcttgacg ccgaatcgtt ctgcaccgtc
      241 gtcgagtccc tggccgcaag gctggaggtt ttgagaactc ctttcatgcc ggttgccgac
      301 tggggaatgg cgctgacgtc gctcttcgac gacttcctgt ccgttcccgc cgatcgcaag
      361 cccgaggaac ttgttcgcca gtcgttggtg tctggattgc agttctattt cggcggccgg
      421 tcgatactgg attccagcct gaagaccgtc ggcgccagcg aagaaaaccg gttgattccg
      481 gcgcacctgg ccttcgagat tgttcgcggt cttgccggaa gcatcccgac cgggcgcggc
      541 gcatatctgt ccgagggcgt gactgtcgcg acactgaagc cgatgcgccc gattccgttc
      601 aaggtgacat tcatccttgg cctggaagag ggcggtttcc cgggggccgc gagggaatcg
      661 tcgcttgacc ttcgaggcgg cgacagacgg attggcgacg tgactacgcc ggactcgggt
      721 cgtcacatgt tcatggagac gctgctttcg accacgcaga gcctggtgtt gagctatgac
      781 gcatgggaca cgatgaagga cgagcgtcac cagccgtgtt cggtggttcg ccagcttatc
      841 gacttcgtcg aggcgaacgt gctgccgggg cagggcgacg acggtggttt caaggaactg
      901 gaaattcccc tttcgccatg gggtgaaaga taccttggcg ccgacgcgcc ggttccgcag
      961 ggtgcgacac gaatcgccga caggcttgtg gccgggctgc ttggggccgc aggttccgcg
     1021 cgcgcgcccg ggctggccgc ggcgattgaa ggggctgtcg ggaatgtcgc tgtttccgga
     1081 cgtgacaggc agcttctccg ggcggcgctc gatctggttt ccgaccatcg cgattgccgg
     1141 cgcgcagatg gatccgcgat ggctgaaccc ggtgattcgt ccacgcccga atatgattcc
     1201 gggccgatca ccgtaaccat ccaggaactg aaccgcttcc tgcgcgatcc gttgaaggcc
     1261 cggctggccc ggttcttcgg gcgccaggac gacctggata agcccgatcc gtccggcctg
     1321 gaagatgaag gattcgaatt cgagacgttt accggtcgaa cactgaagga aaactggctc
     1381 aaggacatcc tgggcggcat ggatccggat cgggcgtggg cgcggcttga aaattcgggt
     1441 cgagcccttt ccatgagatc cagcgcgcct tcacc
//
LOCUS       DAOPPA010000623         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_95447_length_1454_cov_2.929235, whole genome shotgun sequence.
ACCESSION   DAOPPA010000623 DAOPPA010000000
VERSION     DAOPPA010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95447_length_1454_cov_2.929235"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1454)
                     /locus_tag="PKH54_13695"
     CDS             complement(<1..>1454)
                     /locus_tag="PKH54_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD00996.1"
                     /translation="LKTVPAVLLSAAGGLMILLSFPRFDQSWLAWGAAGCMMAAFRGR
                     RFGGAFAIGMVAGFIGNMGGFYWISNLLVDFAHIPLWAAWIVTCLLVAYQGIYYALAA
                     GLSAAVTTKFPKVPWVLAFPVSYTAFEFVFPLIFPWYAANGQQPIIHVIQIADIAGAP
                     AISFLLMTVGTAAGEVVIQVVKKRPFPILSTAVAAVLLTSTLIYGAVRIHTVDERVAA
                     APKFRIGMVEPDVGIWEKEAKHPDGSPMTNRERFDLRSQNLLKLQYLSRAVELEHAPD
                     LIVWPETSYQPNRQVLVRRRNSAPGGEATGTTGAVAIPPELQAEGFIQAWPFPTDMER
                     AFVSRSPLPAQGSFKDPLPALKADSDTPIFDRNAAIRGFSTPVIIGASSRLPDTAPGQ
                     TPVKYNTAVMLDRDGSVIGMYRKTLLLVFGEYLPFERTFPFLRKWLPEAGRWSPGDGP
                     RVFELGQARIGISICYEDLLAPFHKKMSELDPNV"
BASE COUNT          287 a          459 c          465 g          243 t
ORIGIN      
        1 tacgttcggg tccagttcgg acatcttttt gtgaaacggc gccagaaggt cttcgtaaca
       61 gatggatatc ccgattcttg cctggcccag ctcgaagacg cgcggcccgt cgcccgggct
      121 ccagcggccc gcctccggca gccacttgcg caggaacggg aaggtacgtt caaacggcag
      181 gtactcgccg aaaacaagca gcagggtctt ccggtacatg ccgatgacgc tgccgtccct
      241 gtccagcatc acggcggtgt tgtacttgac gggcgtctga cccggggcgg tgtccggcag
      301 gcggcttgaa gccccgatga tgacgggcgt cgagaacccg cggatggcgg cgttcctgtc
      361 aaagatcggc gtgtccgagt cagccttcag ggccggcagg gggtccttga aggatccctg
      421 tgccggaagc ggcgaacgcg acacgaaggc gcgttccatg tcggtcggga aaggccaggc
      481 ctggatgaag ccttccgcct gaagctcggg cgggatcgcc acggccccgg tcgtcccggt
      541 ggcttcgccg ccaggggcgg aattcctgcg gcgcacaagg acctgcctgt tcggctggta
      601 cgatgtttcg ggccaaacga tcaaatctgg cgcgtgctcg agttcgacgg cacgcgacag
      661 gtactgcagc ttcagcaggt tctggcttcg aaggtcgaac ctttcccggt tcgtcatcgg
      721 ggatccgtcg ggatgcttcg cttccttttc ccagatgccg acgtccggtt cgaccatgcc
      781 gatcctgaat ttcggggcgg cggccaccct ttcgtcaacc gtgtggatcc tgacggcgcc
      841 ataaatcagc gtcgatgtaa gcagcacggc cgcgaccgcc gttgaaagga tcgggaaggg
      901 cctctttttg acaacctgga tgaccacctc gcccgcggcg gttccgacgg tcatcagcaa
      961 aaaggaaatg gccggcgcgc cggcgatgtc ggcgatctgg atgacgtgga tgatcggctg
     1021 ctggccgttg gccgcgtacc acgggaatat aagcggaaac acgaactcga acgcggtata
     1081 ggaaacaggg aacgcaagca cccacggcac cttcgggaac ttcgtcgtga cggccgccga
     1141 aagtccggcc gccagcgcgt aatagatccc ctggtacgca accagcaggc acgtcacgat
     1201 ccaggcggcc cacagcggga tgtgcgcgaa gtcaaccagc aggttggaaa tccagtaaaa
     1261 gccgcccatg ttgccaatga atccggcaac catgccgatg gcgaaggccc cgccgaaccg
     1321 tcggccgcgg aacgccgcca tcatgcatcc ggccgcgccc catgcaagcc aggactggtc
     1381 gaacctggga aacgacagaa ggatcatcag cccgcccgcc gccgacagca ggaccgccgg
     1441 caccgtcttc aaca
//
LOCUS       DAOPPA010000624         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_95626_length_1452_cov_4.434503, whole genome shotgun sequence.
ACCESSION   DAOPPA010000624 DAOPPA010000000
VERSION     DAOPPA010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95626_length_1452_cov_4.434503"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKH54_13700"
     CDS             complement(<1..937)
                     /locus_tag="PKH54_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00997.1"
                     /translation="MRRKHSTNWLIAVLLALVSAGCGSSQGRDVAADVPEEVYSDAGS
                     DVPSDVPFDAAEDNGLPDLPDSSDDASDPGDLADYDVAFDADADCHVGDATGDTCQGD
                     AACLYEEGVQRLESEAAGLTMELDQWGGWINHPEEFGEIEATGKFGVRKLGGAWWFVT
                     PDGNPMISKGVTDVNWLGATLQDDAFHQILVDKYGDEDAWNDAAAERIREWNFNTIGP
                     WSSFGISQRFAHATVILDSANHAPRYDPGDVVTDYFAEPFIQHCDKVAGERAAPYVGN
                     SGFIGYFLDNELSWGPDWRGELTLLQRYVDFPVDAP"
     gene            complement(1037..>1452)
                     /locus_tag="PKH54_13705"
     CDS             complement(1037..>1452)
                     /locus_tag="PKH54_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00998.1"
                     /translation="IGVPVRVAGTLDVSIEAGCELYLASGLNMKFSVINISGGGRMPL
                     AGTLDMAGGTLVVDGRGTLSIGPGTVCLISDATLEFKPGAEADMSVGKVYPLISGAAN
                     LNATFGTVLLPDPGPGRAWDQTRLYIDGTVKVVGI"
BASE COUNT          301 a          469 c          411 g          271 t
ORIGIN      
        1 ccggggcgtc caccgggaaa tcgacataac gttgaagcag ggtcagctcg ccccgccagt
       61 ctggccccca cgaaagttcg ttgtccagga aataaccgat aaatccggaa tttccgacat
      121 acggcgcggc acgctcgccg gccaccttgt cgcagtgctg gatgaacggt tcggcgaagt
      181 agtcggtcac aacgtcgccc ggatcatagc gcggcgcgtg gttggccgag tccagaatga
      241 cggtcgcgtg ggcaaaacgc tggctgatgc cgaaactgct ccacggcccg atggtgttga
      301 aattccattc ccggattcgc tcggcggcag cgtcgttcca ggcgtcctcg tcgccgtact
      361 tgtcaaccag tatctggtgg aacgcgtcgt cctgcagggt tgcgcccagc cagttgacgt
      421 cggttacgcc ctttgaaatc atggggttgc catcgggagt cacgaaccac caggcaccgc
      481 ccagcttccg gaccccgaac tttccggtcg cctcgatttc accgaattct tcaggatggt
      541 ttatccagcc gccccactga tccagttcca tcgtaagccc ggcagcctcc gattccaggc
      601 gttgcacacc ctcttcataa aggcatgccg cgtcaccctg gcacgtgtcc ccggtcgcgt
      661 cgccgacatg gcaatcggca tcggcatcga aggccacgtc ataatcggcc agatccccgg
      721 ggtcggaagc gtcgtcggat gaatcgggca agtcgggcag gccattatct tcggctgcgt
      781 cgaacgggac gtcggacggg acatcggatc cggcgtcgga atacacctct tcagggacat
      841 cggccgccac gtcccggccc tggctggacc cgcagccggc cgaaaccaat gccagcagaa
      901 ccgcgatcaa ccagtttgtt gagtgcttcc ttcgcacgcc gttacccttg gaattcaatt
      961 cgataccggc aaagcataca aaaatgacag caactgtcaa attagaaatc agcaatgggg
     1021 cgacgacgaa agtccgtcag atgccgacga ctttgaccgt tccgtcgatg taaaggcggg
     1081 tctggtccca cgccctgccg gggccgggat caggcagcag gacagtaccg aacgtggcat
     1141 tcaggttggc cgcgccgctt atcagtggat ataccttgcc gacgctcatg tccgcttccg
     1201 ccccgggctt gaattcaagc gttgcatcgg aaatcaggca gacagtcccg gggccgatcg
     1261 aaagcgtgcc gcgaccgtca acgacaagcg tgccccccgc catgtcaagc gttcccgcca
     1321 ggggcattcg ccccccgccg gaaatgttga tgacggaaaa cttcatgttc agtcccgatg
     1381 ccaggtacaa ctcacagccc gcttcgattg acacgtccag tgtcccggca acacgaaccg
     1441 gaaccccaat ct
//
LOCUS       DAOPPA010000625         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_97552_length_1432_cov_3.737836, whole genome shotgun sequence.
ACCESSION   DAOPPA010000625 DAOPPA010000000
VERSION     DAOPPA010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97552_length_1432_cov_3.737836"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1432
                     /locus_tag="PKH54_13710"
     CDS             <1..>1432
                     /locus_tag="PKH54_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD00999.1"
                     /translation="LHEVYGPDYDRPDLETFSDIRFMATVNREPQKVQTSRIGRFSRS
                     SIVGITNVGQDLKVSAVFAAPMAGDTKFDTSLVAVFRIQNTGSTPIPAVGLAMKAKSG
                     FEQSAEGAVVIQRDLRRIWTICPEAAWNPDSRTFEITAGTLEPGARRDVTFWMFLTDG
                     EPAVDPTPYFRQVAAEADTREVLSATDSWWKEWFNDGLQIEIGDPMVTDLLESFLYTV
                     KSQIGHNGAMTPLSHYGEYWTRDLSGAARLFAIGSRPDELKAMLDYHHVAACANQGFF
                     NASKVNWPLGVQCAVTDWSSLPVMTEKTAAEAPSYVVHMYHRLFDATGDDSLMREREQ
                     MLRYGLQKQAMNAEFLLPFSDDETFRPAMAMNLGIGGPETMFATLAWSLHSTLLYVAA
                     ADGLARALGYQDGDDIIQLRDNVAATIESRYWQQAEGFFAPILYMSDLSPHMVPYEDV
                     NLTASWMNMEDFLGWDRVRSDVGVLAG"
BASE COUNT          283 a          431 c          440 g          278 t
ORIGIN      
        1 ctccacgaag tctatggtcc ggactacgac cgtcccgacc tggaaacctt ttccgacatc
       61 cgcttcatgg cgaccgtcaa ccgggaaccc cagaaggttc agacatcgcg gatcggacgt
      121 ttcagccgca gttccattgt cggcatcacc aacgtcggcc aggatttgaa ggtttcggcg
      181 gttttcgcgg cgccaatggc cggggacaca aaattcgata catccctggt ggcggttttt
      241 cgcattcaga acaccggctc gacaccgatt ccggcagtcg gcctggcgat gaaggcgaag
      301 tcgggattcg agcagtccgc cgagggcgcc gtcgtgatac agcgggatct gcgccgaatc
      361 tggacgatct gccccgaagc cgcgtggaac cccgattcaa gaaccttcga gatcactgcc
      421 gggacgcttg aacccggcgc caggcgcgac gtgacgttct ggatgttcct gaccgatggc
      481 gagccggccg tcgatcccac cccgtatttc aggcaggtcg ccgccgaggc cgacaccagg
      541 gaagttcttt cggcaacgga ctcctggtgg aaggaatggt tcaatgacgg tctccagatt
      601 gagatcgggg atccgatggt caccgacctg ctcgagtcgt tcctttacac ggtcaagtcg
      661 cagatcggac acaacggcgc gatgacgccg ctttcccatt acggcgaata ctggacgagg
      721 gatctcagcg gggccgcgcg ccttttcgca atagggtcgc gcccggacga actgaaggcg
      781 atgcttgact atcaccacgt cgcggcctgc gccaaccagg gcttcttcaa cgcctcgaag
      841 gtcaactggc cgctgggcgt ccaatgcgcc gtgaccgact ggtcttcgct gcccgtcatg
      901 acggaaaaga ccgcggccga ggcaccatcg tacgttgtcc acatgtatca caggctgttc
      961 gacgccaccg gcgacgattc gctgatgcgg gaaagggaac agatgctgcg atacgggttg
     1021 cagaagcagg cgatgaacgc cgaattcctg cttccgtttt ccgacgacga gacattcagg
     1081 ccggcaatgg cgatgaacct tggcatcggg gggccggaaa cgatgtttgc aacgcttgcg
     1141 tggtcgctcc attcaacgtt gctgtacgtc gcggcggccg acggcctggc ccgcgccctt
     1201 ggttaccagg acggcgacga catcattcaa cttcgggaca acgtggcggc gacgattgaa
     1261 agccgttact ggcagcaggc tgaagggttt ttcgcgccga ttctttacat gtccgacctt
     1321 tccccgcaca tggttccgta cgaggacgtc aacctgaccg caagctggat gaacatggag
     1381 gacttccttg gatgggatcg ggtgcgcagc gacgtgggtg tccttgccgg ac
//
LOCUS       DAOPPA010000626         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_97965_length_1428_cov_2.723962, whole genome shotgun sequence.
ACCESSION   DAOPPA010000626 DAOPPA010000000
VERSION     DAOPPA010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97965_length_1428_cov_2.723962"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKH54_13715"
     CDS             complement(<1..371)
                     /locus_tag="PKH54_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01000.1"
                     /translation="MKTGQKRIVRAFVIIAVTAAFLIPDVITKRWAEDNLANWGHMLA
                     VKPAAGAATVGDAVRQRFPEMKDAGLQGILFKLPDKVAIEPTDKVHELDNKSGFAVEG
                     FLAFDPDGRFARRVQRLDSQIV"
     gene            complement(383..>1428)
                     /locus_tag="PKH54_13720"
     CDS             complement(383..>1428)
                     /locus_tag="PKH54_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOD01001.1"
                     /translation="RQQAPFRAVLTHGFVVDGNGRKLSKSEGNYIDPAKIIDRDGADV
                     LRLWTAAEDYRDDIRLSQEILTRLVEAYRKIRNTTRFMLGVISDFDPVADAIPDSELL
                     EQDRWALARFGEVVAKVRESYDGYEFHNVVRTILAFMVSDLSQFYLDITKDRLYCSHP
                     AGRDRRSAQTAIYRMLDAMIRLMAPVLSFTMEEAWSHHRRMPGSPDSVLLSGLPCADA
                     FPKEAELLADMALLREVRDVVLKNLEEKRAARDIGHPLEASVLVRVKAGGEHDRVVAR
                     FADILPELFVVSRTTVERVDSIAPDVAAEAVVTPIVGERCARCWNYFETVGSVESHPD
                     LCTRCADVIDSIG"
BASE COUNT          287 a          467 c          395 g          279 t
ORIGIN      
        1 acgatctgcg agtcaagacg ttgaacccgg cgcgcaaaac ggccatccgg gtcaaatgca
       61 agaaagcctt caaccgcgaa tccggatttg ttatccagtt catgcacctt gtcggtcggt
      121 tcgatcgcaa ccttgtcggg aagcttgaaa aggatcccct ggagtccggc gtccttcatc
      181 tcgggaaacc gctgcctgac cgcatccccg acagtcgccg cgcctgccgc cggtttcacc
      241 gcgagcatgt ggccccagtt cgccaggttg tcttccgccc atcgcttggt aatcacgtcg
      301 ggaatcagga aggccgccgt caccgcgatg atgacaaatg ccctgacgat tctcttctga
      361 ccggtcttca tctggttccc cttcatccaa tgctgtcgat cacgtcggca cacctggtgc
      421 acaaatccgg atgggattcg accgaaccga cggtttcaaa gtaattccag caccttgcgc
      481 aacgctcgcc gacaatcggc gtaacgaccg cctcggccgc gacgtccggg gcgattgaat
      541 cgaccctctc aaccgtggtt ctggacacga cgaaaagttc cggaagaatg tccgcgaacc
      601 tggccacgac cctgtcgtgc tctccaccag ccttgacgcg aaccaggacc gacgcctcga
      661 gtgggtgtcc gatatcgcgg gccgccctct tttcttccag gttcttcaga acgacatccc
      721 tgacttcacg cagcagcgcc atgtcagcca gcagttcggc ctccttcggg aaggcgtccg
      781 cgcaaggcag ccccgacagc aggaccgaat ccggtgaacc cggcatgcgt ctgtgatggg
      841 accacgcttc ctccatggtg aaggaaagca ccggcgccat cagccgaatc atcgcatcca
      901 gcatcctgta gatcgccgtc tgggccgatc tcctgtcgcg accggccggg tgggaacagt
      961 agagcctgtc cttcgtgata tccaggtaga actgcgaaag atccgaaacc atgaacgcaa
     1021 ggatcgtcct gacgacattg tggaactcgt atccgtcata cgattcgcga accttcgcga
     1081 cgacttcgcc gaaccgcgcc agcgcccacc gatcctgttc cagaagttcc gaatccggaa
     1141 tggcgtcggc cacgggatcg aaatccgaaa taacgccgag catgaagcgc gtggtgttgc
     1201 ggatcttgcg gtacgcctcg acaagcctgg tcaggatttc ctgagaaagc cggatgtcgt
     1261 cccgataatc ctcggccgcg gtccacaggc gcagcacgtc cgcgccgtcg cggtcgatga
     1321 tcttcgcggg gtcgatgtag ttgccctcgg acttcgacag cttgcgcccg ttgccgtcaa
     1381 cgacgaatcc gtgtgtcagt accgccctga acggggcctg ctgcctcg
//
LOCUS       DAOPPA010000627         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_98267_length_1425_cov_2.991241, whole genome shotgun sequence.
ACCESSION   DAOPPA010000627 DAOPPA010000000
VERSION     DAOPPA010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98267_length_1425_cov_2.991241"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>1425
                     /locus_tag="PKH54_13725"
     CDS             44..>1425
                     /locus_tag="PKH54_13725"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOD01002.1"
                     /translation="MSRTRKLQWVLYPSFLVVISVCLLFVGTSTVISVRAMFVRETTE
                     SLRGRVHLLSELLAEFIPGQMEMAERLVSAAGINLATRVTLILPDGKVVADSQEEPAA
                     MQNHLDRPEIRDALALGEGTARRRSPTQGRDTIYHARSIVREGRPLAVVRVGLPEHEV
                     DAALLDLAVKTMAGGLVAAVLAAFLSWLVSRRVARPVEEMAAEVKEMISEGSETRLKP
                     PNIHEFSTLAGTMNMMADGLRERLVATGAQQRELETTLSGMSEAVLLMDAADRIRIIN
                     PAAERLLRLRHGSSTGLRIFDILRDGPLFEFVSRPMSREEAIEEEISIEGDKPTYVHA
                     FRTSIPDDQGSSRGTLLVLHDITRLKRLEAIRRDFVANVSHELKTPITSIKGFVETLR
                     SDTDHDPETVRRFLDIIARHTDRLNSIIEDLLVLSRIEQAENLGRDKDGEDPGMAREN
                     VLVSSIVESV"
BASE COUNT          284 a          397 c          446 g          298 t
ORIGIN      
        1 cgcggcgatg aaggccagcc gctttttgtc ggagacgatt ccaatgtcca ggactagaaa
       61 gctccaatgg gttctttatc cgtccttcct ggttgtcata tcggtctgtc tgctgtttgt
      121 cggtacttcg acggtcatat ccgtccgcgc catgtttgta agggaaacca ccgagagtct
      181 gcgggggcgg gttcacctgc tctccgaact gctggcggag ttcattcccg gccagatgga
      241 aatggctgaa cgcctggtca gtgcggccgg aatcaacctt gcgacccggg tgaccctgat
      301 cctgcccgat ggcaaggtcg ttgccgattc ccaggaggag ccggccgcga tgcagaatca
      361 ccttgatcgt cccgagattc gtgacgccct ggcgcttggc gagggaacgg cgcgccgtag
      421 aagtccgacc cagggcaggg acacgatata tcatgcaaga tccatcgttc gcgaggggcg
      481 gccgctggcg gtcgtgaggg tggggctgcc cgagcacgaa gtggatgccg cgctgctcga
      541 tctggccgta aagaccatgg ctggcgggct tgtggccgcc gtgctggccg ccttcctttc
      601 ctggctggtt tccagacggg tcgcgcgacc ggtggaggag atggcggccg aagtcaagga
      661 aatgatttcc gagggatccg aaaccaggct caagccgcca aatattcatg agttttcaac
      721 ccttgccggc acgatgaaca tgatggccga cggtctgcga gagcggcttg tcgcaaccgg
      781 ggcgcaacag agggaactcg agacgacgct ttccggaatg agcgaggccg tactcctgat
      841 ggacgcggcc gatcgcatca ggatcatcaa tcccgccgcc gaaaggcttc tgcggctgag
      901 acacggctcg tcaacgggac tgcggatatt cgacattctt cgggacgggc cgctttttga
      961 gttcgtctcc aggccgatgt cccgggagga agcgattgag gaggaaatca gcatcgaagg
     1021 cgacaagccg acttatgtgc acgcctttcg aacttcaatc ccggatgatc agggttcaag
     1081 caggggcacc ttgctggtgc ttcatgacat cacgcgcttg aagcgtcttg aagcgattcg
     1141 ccgtgatttc gtcgcaaacg tcagtcacga attgaagact ccgattacct ccatcaaggg
     1201 gtttgtcgag acgctgcgtt cggatacgga tcatgacccc gaaaccgttc gccgtttcct
     1261 cgatatcatc gccaggcata ccgatcgcct gaattcaatc atcgaggatc tgcttgtcct
     1321 ttccaggatc gagcaggcgg aaaacctggg gcgtgacaag gatggcgaag acccgggaat
     1381 ggcccgcgag aacgtgcttg tttcgtccat cgtcgagtcg gtgat
//
LOCUS       DAOPPA010000628         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_98913_length_1419_cov_1.792522, whole genome shotgun sequence.
ACCESSION   DAOPPA010000628 DAOPPA010000000
VERSION     DAOPPA010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98913_length_1419_cov_1.792522"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKH54_13730"
     CDS             <1..659
                     /locus_tag="PKH54_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HOD01003.1"
                     /translation="QRIGLHADPETKVRKLIDDHIISLGIDPKIPPMAITDADFDQHL
                     DKHRSPKAKASEMEHALRYHISKHLNEDPELFEALSKRLDRILTQLKDRWEELVAALR
                     EFSDEVRAGRQEDGTGLDPETQAPFLGVLRQEVGKGGEVNGEDMRKLVEATVGLVDHI
                     KQEIGLIGFWSRAQAQDALRSWIVQYLDELEILPYDRLPAMADRLLELAKVNHHKLVK
                     "
     gene            656..1354
                     /locus_tag="PKH54_13735"
     CDS             656..1354
                     /locus_tag="PKH54_13735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008008994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOD01004.1"
                     /translation="MSPTLTVDDLAFEVLPSPRRKTVELTVERDGHLTIRAPEGTDDS
                     VLTAFVQEKRFWLYQKIALKNSMRQAVATKEFVSGEGFPYLGRSYRLLLVDDQDVPLK
                     LEAGRFRMPRAETERGREIFIAWYAEHALPWLKNRVRVFASRVGVEPARVEVRDLGFR
                     WGSCGRAGTLNFNWATILLPPAIVEYVVVHELVHLHEDNHTPEFWQRVERVLPDYKQR
                     KQWLAEKGAGYVVV"
BASE COUNT          315 a          384 c          463 g          257 t
ORIGIN      
        1 tgcagcgcat cgggctgcat gccgacccgg aaacgaaggt caggaagctg atcgacgacc
       61 acatcatcag cctgggaatc gacccgaaga ttccaccgat ggcgatcacg gatgcagatt
      121 ttgaccagca ccttgataag caccgcagtc cgaaagcgaa ggcgtccgaa atggaacacg
      181 cactgcggta tcacatcagc aaacacctga atgaagaccc ggagctgttc gaggctttgt
      241 cgaaacggct tgaccgcatc ctgactcagt tgaaggaccg ctgggaagaa ctggtggcgg
      301 ccttgcgcga gttttcggac gaagtgcggg cgggcaggca ggaagacggc acagggctgg
      361 accccgagac acaggcgccc ttccttggcg ttctgcgaca ggaagtcggc aagggcggcg
      421 aggtcaacgg tgaagacatg cggaagcttg tcgaggccac cgtcggcctg gtggaccaca
      481 tcaagcagga aatcggcctg atcggtttct ggtcgcgggc acaggcacag gatgcgctcc
      541 gaagctggat cgtgcagtac ctggatgaac tggaaatcct gccgtatgac cggcttccgg
      601 ccatggccga caggctcctt gagctcgcca aggtgaacca tcacaagctg gtgaaatgag
      661 cccgacgttg acagttgacg acctcgcgtt cgaggtcctg ccaagcccga gacgcaaaac
      721 tgtggaactg acggtcgagc gtgacgggca tcttacgatc cgggcaccgg agggcacgga
      781 cgattccgtg ctgacagctt tcgttcagga aaagcggttc tggttgtatc agaagatcgc
      841 cctgaagaat tccatgcgac aggctgtggc gacgaaggaa ttcgtgtccg gcgagggctt
      901 cccttacctg ggacggagct accgcctgct actggttgac gaccaggacg ttcccctgaa
      961 gctggaagcc ggaagattca ggatgccccg ggcggagact gagaggggac gggaaatctt
     1021 catcgcctgg tatgcggaac acgcccttcc gtggctcaag aaccgcgtgc gggtgttcgc
     1081 gtcaagggtc ggtgtcgaac ccgccagggt ggaagtgcgc gacctaggct tcaggtgggg
     1141 gtcatgcggt cgggccggaa ccttgaattt caactgggcg accatcctgc tgcccccggc
     1201 catcgtcgaa tacgtcgtcg tccacgaact ggtccacctg cacgaagaca atcacacgcc
     1261 ggaattctgg cagcgcgtcg aacgtgtcct gcccgattac aagcagcgca agcagtggct
     1321 ggcagaaaag ggtgcggggt atgttgtggt gtaatggtgt cttggttatg gatcatagta
     1381 gaagggatct tgggggttgg gcaatcaccc aagacctgt
//
LOCUS       DAOPPA010000629         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_100573_length_1402_cov_3.510765, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000629 DAOPPA010000000
VERSION     DAOPPA010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100573_length_1402_cov_3.510765"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKH54_13740"
     CDS             <1..786
                     /locus_tag="PKH54_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOD01005.1"
                     /translation="GLGMVAVRNSTYYGIAGYFTLMAIDAGMIGITGTNARPSIAPTF
                     GVENMLGTNPLTFGIPTDEEFPFVIDCATSVTQRGKIEVYDRLGKTMPEGWVIGEDGK
                     TRTDPTQTLKDLVKGTAALTPVGGLGEDGGGYKGYGWATVVEILSAALQNGSYLKQLL
                     GIEDGKKVPYHLGHFFIAIDIEHFINLDIFKKISGSICRDLRNSKKAPGQDRIWTAGE
                     KEHDAWLFRKDRGVPVNAPLMKNCNDMRRELGLTQYHFPWDDE"
     gene            835..>1402
                     /locus_tag="PKH54_13745"
     CDS             835..>1402
                     /locus_tag="PKH54_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOD01006.1"
                     /translation="MSGGPAIVLVDPSNPQNIGAVCRAMANCGLTDLRLVRPVEGWNC
                     GEARALAVSAKGMLETAKVYSTTAEAVGDCTHVFATTARRRDQVIPEMTARRAASEIR
                     ALKKVGAGVAILFGAERTGLQNQDIALSNVVITVPLNPGMSSLNLAQAVLLVAYEWLQ
                     AGMDDPVEIVDPTIGRMPAKKQQMEVFFQ"
BASE COUNT          289 a          414 c          451 g          248 t
ORIGIN      
        1 ggtctgggca tggtcgctgt caggaattcg acctattacg gcatcgccgg gtacttcacg
       61 cttatggcta tcgacgctgg catgattgga atcaccggca cgaacgcccg tccttccatc
      121 gccccgacgt tcggcgtcga aaacatgctg ggaacgaacc cgttgacctt cggcatcccg
      181 acagacgagg aattcccgtt cgtcatcgac tgcgccacct cggtgaccca gcgcggaaag
      241 atcgaggttt acgacaggct tggcaagacc atgccggaag ggtgggtcat cggcgaggac
      301 ggcaagacgc ggaccgaccc gacccagacc ctgaaggatc tggtcaaggg aacggcggca
      361 ctcaccccgg tcggcggcct gggcgaggac ggcggcggat acaagggcta cggctgggca
      421 acggtcgtcg aaattctttc ggccgccctg cagaacggca gctatctgaa gcagctcctt
      481 ggcatcgagg acggcaagaa ggttccgtac cacctgggac acttcttcat cgcgatcgat
      541 atcgagcact tcatcaacct ggatatcttc aaaaagattt ccggcagcat ctgccgcgac
      601 cttcgcaatt cgaagaaggc cccgggccag gatcgcatct ggacggccgg cgagaaggaa
      661 catgacgcgt ggctgttccg caaggatcgc ggggtcccgg tcaacgcgcc gctgatgaag
      721 aactgcaacg acatgcgccg cgaactgggc cttacgcagt atcatttccc gtgggatgat
      781 gaatagtctt tccgggaatc cgaagtggcg ataaccaccc caaccgaaga aatcatgtca
      841 ggcgggccgg cgattgtgct ggtcgatccg tcaaatcccc agaacattgg cgcggtgtgc
      901 cgggccatgg ccaactgcgg cctgaccgac ctgaggctgg ttcgaccggt cgagggctgg
      961 aattgcgggg aggcccgcgc cctggcggtc agcgcgaagg gcatgctgga aaccgcaaag
     1021 gtgtattcga cgacggctga agcggtcggt gactgcacgc acgtgtttgc gacaacggcc
     1081 cgcaggcgtg accaggtcat tcccgagatg accgcgcgca gggcggcttc ggaaatacgg
     1141 gcgttgaaga aggtcggggc cggggttgcg atactgttcg gggctgaacg caccgggctt
     1201 cagaaccagg acatcgcgct gtcgaacgtg gtgatcaccg ttcccctgaa cccgggcatg
     1261 tcgtccttga accttgccca ggccgttctg cttgtcgcct acgagtggct gcaggccggc
     1321 atggacgatc cggtcgagat cgtcgatccc accattggcc ggatgccggc gaagaagcag
     1381 cagatggaag tcttcttcca gc
//
LOCUS       DAOPPA010000630         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_101113_length_1397_cov_1.949329, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000630 DAOPPA010000000
VERSION     DAOPPA010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101113_length_1397_cov_1.949329"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..966)
                     /locus_tag="PKH54_13750"
     CDS             complement(121..966)
                     /locus_tag="PKH54_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOD01007.1"
                     /translation="MTSASTIPADPARIDPSIRAVIMDRLSAIESEHGVRILFACESG
                     SRGWGFASPDSENDVRFIYVHPVEWYLSLSPGPDVIELPVDQVYDIGGWELRKALNLL
                     KKGNPTVVEWLNSPVTYRSEPGFLRGIRSAATAVHRPEKSLHHYLHMAQGNFHKYLCG
                     DRINLKKYLYVLRPLLACMWIRSEYGIPPMRFQDLVDRMVTDSELCQAIDELLARKAL
                     ISESACGHPIPLLHNFITATLAGLEAMPAQPESLRPDFTILDRLMVDTVLESVLPVTG
                     HAPDE"
BASE COUNT          287 a          379 c          449 g          282 t
ORIGIN      
        1 ctgtgtcagg ctgcaagcga cgttccttcg tacaccactc ccctggactc taactcaaac
       61 aggaacggga attcccaacc gcagttgttc aggatttctg cgcgtaagcg ttcgtcatgt
      121 tcattcgtca ggggcatgcc cggtcacggg cagtacggat tccagcaccg tatccaccat
      181 caaccgatcc aggatggtga aatccgggcg cagagattca ggctgggcgg gcattgcctc
      241 aaggccggcc agtgtggccg tgatgaaatt gtgcagaagc gggattgggt gaccgcaggc
      301 actttcagat atcagggcct tgcgggcaag aagttcgtcg attgcctggc acagttcgct
      361 gtcggtaacc atccggtcca ccaggtcctg aaagcgcatc ggcggaatgc cgtattcaga
      421 ccggatccac atgcaggcca gaagcgggcg taaaacatac agatatttct tgaggttaat
      481 ccggtcgccg cacagatact tgtggaagtt gccctgtgcc atgtgcaggt agtggtgcag
      541 tgacttttcc ggacgatgaa cggcggtggc ggccgaacgg atcccgcgca ggaaccccgg
      601 ttcggaacgg taagtcaccg gcgaattcag ccattcaacg accgtcggat tgcctttctt
      661 cagaaggttc agagccttcc gcagttccca accgccgatg tcgtagacct ggtcgaccgg
      721 cagttcgatg acgtcggggc cgggcgaaag cgacagatac cactcaacgg ggtgaacgta
      781 tatgaacctg acgtcatttt cgctgtccgg cgaagcgaag ccccatcccc gacttccgga
      841 ttcgcaggcg aaaaggatgc ggacgccatg ttcggattcg atggcggaca gccggtccat
      901 gatgactgcg cggatggacg gatcgatccg ggcaggatcg gctggaatcg tgctggctga
      961 ggtcatttgt ccccgggttt catgggcgaa cgtcattcgc cggtacaagg gtgccgggcc
     1021 gggcagaaag gcgccggcct tcacaagttc caagcgtaca tggggatgcg gcaaggttcc
     1081 tgccggaaat cgattaaaca tgtcggatgt tcgagagcca gcctaggccg ctcaccagcg
     1141 gggctagcct gccgagaacc cgggtggtgg tggcacccct catgcaggct acgactctct
     1201 gaacgccttg atatcacgga gttgtatcta agggaagggg tttggtcgcg gggactagtc
     1261 caaatgtccg ggttcgtcgg cgactgtgat ttcgtcgacg accaggcgga agccgatctg
     1321 gttggtgcgg gtgatgtcgg cgcggaagct ttcgcgggat gcggcgcggc atgaaatgcg
     1381 atcgccctgt gcggagc
//
LOCUS       DAOPPA010000631         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_101882_length_1389_cov_4.897301, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000631 DAOPPA010000000
VERSION     DAOPPA010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101882_length_1389_cov_4.897301"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKH54_13755"
     CDS             <1..580
                     /locus_tag="PKH54_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOD01008.1"
                     /translation="FYNETAGTSIQPTPVVGVVGLVEDCDRVAGTFFTREDEAIVLLG
                     CEDFSFDASLFARLKTGKVCGFVRKADAKRHSALCELIVRGIEKGIVTAAHDVSEGGL
                     LWAIAESVVGNGVGATLDNVGTEPAELFGEAPSMAFVTVPEADLSCLTLMAEELGVQA
                     VRIGTTGGDTLNVKGAFTVPVADLTAASRKPL"
     gene            complement(648..>1389)
                     /locus_tag="PKH54_13760"
     CDS             complement(648..>1389)
                     /locus_tag="PKH54_13760"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOD01009.1"
                     /translation="GGLRPKCTVLDEDGSLAIGKFPSISDQRSVTRGEVLAMRLAARA
                     GIRAASARIVMIPDIPVAVIRRFDRASGGGRIHYWSASSMLQSDRGEDRSYTELVSAI
                     ARYCVDAVADSRELWRRIVFNHLITNTDDHLRNTGFLHVGGGLWRLSPAFDLNPFPDK
                     AQESKTWLSEDTGPVTSIRTLIGMASRFYLSRDEALAILREILGALVGWKALSRNRDI
                     GLSDADVRDFESAFDHEEMEVALSLVGR"
BASE COUNT          278 a          457 c          406 g          248 t
ORIGIN      
        1 cttctacaat gaaaccgctg gcacttcgat ccagccgacc ccggtcgtcg gcgtagtcgg
       61 cctggtcgaa gactgcgacc gcgtggcggg gacgttcttc acccgcgagg acgaggccat
      121 cgtcctgctt ggctgcgaag acttcagctt cgacgcgtcc cttttcgcgc gcctcaagac
      181 cggcaaggtc tgcggattcg tcaggaaggc ggacgcgaag cgccactcgg ccctgtgcga
      241 gcttatcgtg agaggtatcg aaaagggcat cgtgaccgcg gcccacgacg tgtccgaagg
      301 cggcctgctg tgggcgatcg ccgaaagcgt ggtcgggaat ggcgtcggcg ccacgcttga
      361 caacgtcggg accgaaccgg ccgaactgtt cggggaagcc ccgtcaatgg cgttcgtcac
      421 cgttccggaa gccgacctgt cctgcctgac cctgatggcc gaagaacttg gcgtccaggc
      481 cgtccgcatc ggcaccaccg gcggcgacac cctgaacgtc aagggcgcct ttaccgtccc
      541 cgtcgccgac cttaccgccg ccagcaggaa gcctctttag agttgcgccg cgataacgac
      601 ttcatcgaat tcgggaaaac cgacggatcg cagcgccgac cgccatgtca ccgacccaca
      661 aggctcaagg caacttccat ctcttcatga tcaaatgcag attcgaaatc cctgacgtcc
      721 gcatccgaca atccgatatc cctgtttctg gacaacgcct tccagccaac cagggcgccc
      781 aggatctcgc ggagtatggc aagggcctcg tcgcgtgaaa gatagaacct cgaagccatg
      841 cctattaagg ttctgatcga cgtcaccggt ccggtgtcct ctgaaagcca tgtcttggac
      901 tcttgcgcct tgtccgggaa ggggttcagg tcgaacgccg gagaaagccg ccacagcccg
      961 cccccgacat gaaggaaccc agtgttgcgc aggtgatcgt cggtgtttgt aatcaggtgg
     1021 ttaaacacga tccttcgcca cagttcccgg ctgtcggcga cggcatcaac gcagtaccgg
     1081 gctattgccg aaaccagttc cgtatatgaa cgatcctcgc cacggtcgga ctgcagcatg
     1141 gaagacgccg accagtaatg aatcctgccg ccgccggaag ccctgtcaaa ccgccgaatc
     1201 accgccaccg ggatgtcggg gatcatgacg attcgtgccg aggccgccct gatgccggcc
     1261 ctggcggcca ggcgcatggc aagaacctcg ccacgggtca cgctcctctg gtcggaaata
     1321 cttggaaact tgccgatggc caggctcccg tcctcatcca ggacggtgca cttcggccgc
     1381 agtccacca
//
LOCUS       DAOPPA010000632         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_103697_length_1372_cov_3.691724, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000632 DAOPPA010000000
VERSION     DAOPPA010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103697_length_1372_cov_3.691724"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1372)
                     /locus_tag="PKH54_13765"
     CDS             complement(<1..>1372)
                     /locus_tag="PKH54_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD01010.1"
                     /translation="NVVPGLIVDEVTWMNSTGAPIGQVTTDMLTSTMKVRIRFYTAYD
                     FDGDITLAVKGDLPGLDQSADPTYGQHTQAFDLDPRLYVTGTRSFIETEFQPGAALVD
                     GFYFELFANGETKPWFTGNWDEIMASGEVPVYQDIYRDNFATYGKWPPSLPMAAGSGS
                     RDCEFMVMARTFPNGGPIPDVTVTVGPAGDPGDGDAESTRTRANGIAAFDLVEPGQYV
                     ITGRPDDTKGSRFVAIDENPTPVECPAGGGTSWTMLQYHVIPRITVPGGISGRNDCVV
                     FNTNSDEFWGRAEQFMITITNYYDETVIAPEVAKKARPRIEACFPSPLENGTTIQIRA
                     VPVYVNGAGTGVEGFSGPILVDSTVPVVDDVQFAATATTCQEGAAVFPFEVDIYVSAT
                     TGPIDVVELQNDEDGWDILQFESVPGEMPGTERLTVDFDADGRKPGDTLLFRLTNRAG
                     VFTEYPV"
BASE COUNT          247 a          403 c          430 g          292 t
ORIGIN      
        1 acggggtatt cggtgaatac gccagctcgg ttggtcagcc tgaagagcag ggtgtcgcct
       61 ggcttgcggc cgtccgcatc gaaatcgacc gtcaggcgtt cagtgccggg catctcgccg
      121 ggaacagact cgaactgaag gatgtcccat ccgtcttcgt cgttctggag ttcgacgacg
      181 tcgatcgggc cggtagtggc gctgacgtaa atatcgacct cgaacgggaa cacggcggcg
      241 ccttcctggc aggtcgtcgc ggtggcggca aactgcacgt catcgactac agggacggtc
      301 gaatcgacca ggatcggccc gctgaatcct tcaacgcccg ttccggcgcc gtttacatag
      361 accggcaccg cccgtatctg tatcgtggtg ccgttttcca ggggcgacgg gaagcaggcc
      421 tcgatccttg gtcgagcctt ctttgcgact tcaggcgcga tcaccgtttc gtcatagtaa
      481 tttgtgatgg tgatcatgaa ctgctcggcc cttccccaga attcgtcgga gttggtgttg
      541 aacacgacgc agtcgttgcg gccgcttatg ccgccgggaa cagtgatcct gggaatgacg
      601 tgatactgga gcattgtcca tgacgtgccg ccgccggccg ggcattcgac gggcgtcgga
      661 ttctcgtcga tggcgacaaa gcgactgccc ttggtgtcgt cgggccgtcc ggtgattaca
      721 tattgtcccg gttcaacaag gtcgaaagcg gcaatcccgt tggccctggt acgcgtcgat
      781 tccgcgtcgc cgtcgcccgg gtcgccggcg gggccgacag tcaccgtcac gtctggaatc
      841 ggcccgccgt tcgggaacgt cctggccatg accatgaatt cgcagtcccg cgaacccgaa
      901 cccgccgcca tcggcagtga ggggggccat ttcccgtagg tggcgaagtt gtcgcggtaa
      961 atgtcctgat ataccggtac ttccccactg gccatgattt cgtcccagtt gccggtgaac
     1021 catggcttcg tctcgccgtt tgcaaaaagc tcgaagtaga atccgtcaac cagggccgcc
     1081 ccgggctgaa attcagtctc gatgaaactg cgcgtgccgg tgacgtacag gcgcgggtca
     1141 aggtcgaacg cctgggtgtg ctggccgtat gtcgggtcgg ctgactgatc caggccggga
     1201 agatcgccct tgacggcaag cgtgatgtca ccgtcaaaat catatgccgt gtagaaacgg
     1261 attctgacct tcatggtcga agtcagcatg tcggttgtga cctgtccaat cggcgccccg
     1321 gtcgagttca tccacgtgac ttcatcgacg atgaggcctg ggacgacgtt gt
//
LOCUS       DAOPPA010000633         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_103912_length_1370_cov_4.172624, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000633 DAOPPA010000000
VERSION     DAOPPA010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103912_length_1370_cov_4.172624"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKH54_13770"
     CDS             <1..409
                     /locus_tag="PKH54_13770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD01011.1"
                     /translation="SYPGRSVGPKPKKIVVLDFFSTNCVSCKKMLPALKVFSEQYKDK
                     VQVLLVAVPENGDVGGKKLRAFFEREPMPFPVLEDPSAYYSKKWLPEVNGKAEMPCLF
                     LIDREGIVRAQAPGYHESIADALSPALESALKL"
     gene            486..>1370
                     /locus_tag="PKH54_13775"
     CDS             486..>1370
                     /locus_tag="PKH54_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD01012.1"
                     /translation="MQNSIQDVTIIGGGVIGCAIARELSRYHLKVTLLERHAECGFGT
                     TKTNSGIIHPGHDTPLSTLKGRLVVRGNQMFDQLQKELKFGFERIGAIYWIREQEDRP
                     RLEALLRLGQEKGVPGLEIWDSDRLEREEPNLSRELIAALYAPSGGVINPYEFAFALI
                     ECAQNNGVTLKIDSPVLSIDREDVGDGGLLHVHTPKETVVTRYVVNAAGVYADRVAAM
                     VGLDDFSINPRKGEEFMLDKRLQGIVRRLVFPLPTGVTKGTLIIPTYDGTIMVGPTAH
                     DTGDREDVTTTTDGAAEIF"
BASE COUNT          287 a          389 c          404 g          290 t
ORIGIN      
        1 gtcatacccc ggacgttccg ttggtccgaa gccgaaaaag atcgttgttc tggacttctt
       61 ttcgacgaac tgtgtcagtt gcaagaagat gcttccagcc ctgaaggtct tttcggaaca
      121 gtacaaggac aaggttcagg ttcttcttgt cgcggttccg gaaaatggcg atgtcggggg
      181 aaaaaaactg agggcctttt ttgaaaggga acccatgccg ttccccgtcc tggaggatcc
      241 ttcggcttat tacagcaaga agtggcttcc ggaagtcaat gggaaggctg aaatgccctg
      301 cctgttcctg atcgacaggg aaggcatcgt gcgcgcccag gcgcccggtt atcacgagtc
      361 aatcgccgac gcgctttccc cggccctcga atcggcgctt aagctgtagt ccgctctctt
      421 gccgcgctgc gtcacggcta gtatcattcg aagatctttt tgctgcgctt tacaggaaaa
      481 acgcaatgca gaattcgatt caggacgtca cgatcatcgg cggtggtgtc atcggatgtg
      541 ccatcgcccg ggagctttca aggtatcacc tgaaggttac cctgcttgaa aggcatgccg
      601 aatgcgggtt tggcacgacc aagaccaact ccggcatcat tcacccgggt cacgacacgc
      661 cgctgtcaac cttgaagggg cgcctggttg tccgtggcaa ccagatgttc gaccagcttc
      721 agaaggaatt gaagttcggg ttcgaacgga tcggggccat atactggatt cgcgaacagg
      781 aagaccgccc gcgccttgaa gcgctgctgc gccttggcca ggaaaagggc gttcccggcc
      841 tggagatctg ggattccgat cgtctcgaac gcgaggagcc gaatctttcc cgggaactga
      901 ttgcggccct gtacgcgccc agcggcggcg tcataaaccc gtacgagttc gcctttgccc
      961 ttatcgaatg tgcccagaat aacggggtaa cccttaagat cgacagtccg gtcctgtcga
     1021 ttgatcggga agatgtcggg gacggcggtc ttctgcatgt ccatacgccg aaggagaccg
     1081 tcgtaacccg atatgtggtc aatgccgccg gcgtttatgc cgatcgcgtt gcggccatgg
     1141 tcggacttga cgacttcagc atcaacccgc gcaagggcga ggagttcatg ctggacaagc
     1201 gcctgcaggg aatcgtgcgc cgcctggtgt tcccgttgcc gaccggcgtc acgaagggaa
     1261 cccttatcat tccgacatat gacggcacga tcatggtggg gccgaccgcg cacgacaccg
     1321 gcgatcgcga agacgtcacc acgaccaccg acggcgccgc cgagatcttt
//
LOCUS       DAOPPA010000634         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_103995_length_1369_cov_4.518265, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000634 DAOPPA010000000
VERSION     DAOPPA010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103995_length_1369_cov_4.518265"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKH54_13780"
     CDS             complement(<1..703)
                     /locus_tag="PKH54_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD01013.1"
                     /translation="MFGVVFWVEVGFFGGFHKMFEAIAVAFEAGGPNMFVILAIGIFM
                     WGIIIERLIVLYFKANVDKDGFMRVLTKYILVGDLRNALKLCSISKFPLAKIIQAGLM
                     KAKESDAAVQAAMDEAALREVPRIEKRIGFLAVIGNVGVLAGLLGTIFGLIASFGAAA
                     TADAATKATKLAEGISEAMYNTAFGLGVAILASIFYAFYQAKAQSLVDTINEGTVTTL
                     NLITANRDKFDDKVSE"
     gene            complement(889..>1369)
                     /locus_tag="PKH54_13785"
     CDS             complement(889..>1369)
                     /locus_tag="PKH54_13785"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOD01014.1"
                     /translation="VGYGAGGMGFKGTGSGGGGTGGYGRIHGLGVVDTGGGMGMRAGL
                     GKKGAKKVGKMQIGGMGSSGFCQKSNIEAVVKQRAGAIRACYEAQLQLQEGLAGKLAV
                     RWTINTEGQVDAASVTSSTMGNPKVESCVMTVIRRMRFAKPEGGICVVQWPFVFSPG"
BASE COUNT          291 a          426 c          392 g          260 t
ORIGIN      
        1 actcagagac cttgtcgtcg aacttgtcac ggttggccgt gatcaggttc agcgtcgtaa
       61 cggtgccttc gttgatggtg tcaacaaggg actgcgcctt cgcctgatag aacgcgtaga
      121 aaatcgacgc gagaatggcg acgcccagac cgaatgccgt gttgtacatt gcttcggaaa
      181 taccttccgc gagcttggtc gccttggtgg ctgcgtcggc ggttgccgcg gcgccgaagg
      241 atgcgatcag accgaagatg gttccgagca gaccggcgag aacgccgacg ttaccgataa
      301 ccgcgaggaa gccgatgcgc ttctcgatcc tgggaacttc acgcagggcg gcttcgtcca
      361 tggcagcctg aacggcggca tcggattcct ttgccttcat caggccggcc tggatgatct
      421 tggccagcgg gaacttggag atcgagcaga gcttcagggc gttccgaagg tcacccacca
      481 ggatgtactt cgtcaggacg cgcataaagc catccttgtc aacgttggcc ttgaagtaca
      541 gcacaatcag gcgctcaatg atgatgcccc acatgaaaat gccgatggcc agaatgacaa
      601 acatgttcgg tccgcctgct tcgaatgcaa ctgcgatagc ttcaaacatc ttgtggaaac
      661 ctccgaaaaa acctacctca acccaaaaaa ccaccccgaa cacccttcgg ggcataccat
      721 cagcttcacc tgcccgtgaa attcgttaat aacgagtctg caccggcaag cgaagtttat
      781 agccgtaagg aacagtgctg tcaacctttg ccaagcccgg aaccgcttgt gttcgcgggg
      841 cagggcgttc gccgccaggc ggaacgtccg gcaaagaacg gtgaaacatc agcccgggct
      901 gaaaacgaac ggccactgga cgacgcagat gccgccttcg ggcttcgcga agcgcatacg
      961 acggataacc gtcatgacgc agctttcgac cttgggattg cccatcgtgc tgctggtaac
     1021 cgaagcggca tcgacctgac cttcggtatt gattgtccag cgaaccgcga gcttgccggc
     1081 taggccttcc tggagctgaa gctgggcttc gtagcaggcg cggatggcgc cggcacgctg
     1141 cttgacgacc gcctcgatgt tggacttctg gcagaagccg gacgatccca tgccaccgat
     1201 ctgcatcttg ccgaccttct tggcaccctt cttacccaga ccggcgcgca tgcccatgcc
     1261 gccgcccgtg tcgaccacgc ccagtccgtg gatacgaccg tagccgccgg taccgccgcc
     1321 gccggagccc gtgcccttga agcccatgcc gcccgcgccg tagccgacg
//
LOCUS       DAOPPA010000635         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_104851_length_1361_cov_3.403522, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000635 DAOPPA010000000
VERSION     DAOPPA010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104851_length_1361_cov_3.403522"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1102)
                     /locus_tag="PKH54_13790"
     CDS             complement(65..1102)
                     /locus_tag="PKH54_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD01015.1"
                     /translation="MGRLKGFLWRSDFFPRKLSFSREGKVVVLISIGLGFAAVNTGNN
                     LLYMVFGLSLALILISGFLSEYNIRSIVPGRMGRLRASAGRPASFMLGATNLNKRMSS
                     FGIEVWPLVDGNGVQVEPARFLEMPPGTAADAPGAITFSRRGRYGMHGVVVSTSFPFS
                     FFRKSAVTACSDTVVVHPVVHPIREIELPAAAEGEDESLPRAGRGFEFFGVRDFRVGD
                     NPRNISHRLSAGRTQLIVREFEQTGNKEVMVALVNVDPGGPDGLERAETAVEIAASVA
                     AHLLGKGRVVGLVSSFDAVSPGSGPAQVERILDYLATVPILELNSPGEEMHVRRMLAS
                     LNPGGVVWVRP"
     gene            complement(1124..>1361)
                     /locus_tag="PKH54_13795"
     CDS             complement(1124..>1361)
                     /locus_tag="PKH54_13795"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOD01016.1"
                     /translation="LELGISTRGVVSLASAARASAVVGGRGFCVADDVKSLFVPVCSH
                     RVVVRGHRAGGVSTGTEAAAILSDILLSTPVPGD"
BASE COUNT          297 a          465 c          391 g          208 t
ORIGIN      
        1 cgacccagac gaccgctgcg gtaacaagcc tgtggaggat gtcaaaccgc accgaaaacc
       61 tccatcacgg gcgaacccat acgacaccgc cgggattcag ggatgccagc atccggcgaa
      121 cgtgcatctc ttcccccggc gagttcagtt caaggatcgg aaccgtcgcc aggtaatcca
      181 ggatccgctc gacctgggcc ggcccgcttc ccggggatac cgcgtcgaag gatgagacca
      241 gtccgaccac ccgccccttt cccagcaggt gtgccgccac cgacgccgca atctcgaccg
      301 cggtctcggc cctttcaagc ccatccggtc cacccggatc aacattgacc agggccacca
      361 tcacttcctt gttccccgtc tgctcgaatt cacggactat caactgggtg cgcccggccg
      421 acaggcgatg actgatgttt cgcggattgt cgcccacgcg gaagtccctg acaccgaaaa
      481 actcgaatcc gcgcccggcc ctgggaagac tctcatcctc gccctcggcg gccgcaggca
      541 gctcgatttc cctgatggga tggaccaccg gatgcacgac cacggtatcc gagcatgcgg
      601 tgaccgcgga cttcctgaag aaggagaacg gaaagcttgt tgaaacgacc acgccgtgca
      661 ttccgtaccg gccgcgacgg gaaaatgtta tcgcccctgg cgcatcggcg gccgttccgg
      721 gcggcatctc caggaacctg gccggttcga cctggacgcc gttgccatcg accagcggcc
      781 acacctcgat cccgaacgag gacatgcgct tgttaaggtt cgtcgcgcca agcatgaatg
      841 acgccggcct tcccgccgag gcgcgaagac gacccatcct gccgggaact atcgaacgga
      901 tgttgtactc cgacaggaag ccgctgatca ggattaacgc cagcgacaac ccgaaaacca
      961 tgtagagcag gttgtttccg gtattgaccg cggcgaaacc aaggccgatc gaaatcagga
     1021 cgacgacctt cccctcccgc gaaaacgaga gtttccgggg gaagaagtcg gagcgccaca
     1081 ggaatccctt caatcgcccc atgcggtcac cgtatccgcc agcctagtcg cccgggaccg
     1141 gggtggaaag cagtatgtcg gaaagaatgg ccgcggcctc ggtgccggtc gaaacaccgc
     1201 ccgcgcgatg gccgcgaacc accaccctgt gcgaacagac cggcacaaaa agggatttga
     1261 cgtcgtccgc gacgcagaat ccgcgaccgc cgaccacggc ggaagccctg gccgcggaag
     1321 ccaggctgac gacacccctg gtggaaattc caagttcaag a
//
LOCUS       DAOPPA010000636         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_105152_length_1358_cov_3.554106, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000636 DAOPPA010000000
VERSION     DAOPPA010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105152_length_1358_cov_3.554106"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="glnA"
                     /locus_tag="PKH54_13800"
     CDS             complement(<1..1202)
                     /gene="glnA"
                     /locus_tag="PKH54_13800"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOD01017.1"
                     /translation="MFSTIRELIAYVRKEGVTTVDFKFTALMGKAHHVTLPARKMDES
                     IWESGIGFDGSNMPGFKTVEAGDMVIVPDITTAFIDPFFSEKTLSLICGVYEAGSLDP
                     FPRDPRGILKHACDYMASTGIADDCRFGPEFEFYLLDEVATRTGCAEMSHKLVCREGQ
                     VSAADGARERITIAPHRGYHACPPADAFFNIRNEMAVLLEQAGVGVNYHHHEVGATGQ
                     QEIEVDLAGPMRSGDVAFLVKYFVKMTARKHGMVATFMPKPFFGEAGSGMHFHQHLFK
                     GGQPVFFDKDGYAGLSKTALAYVAGLLHHAPSILAITNPTTNSYRRLVPGYEAPVNAF
                     FSLGNRSAAIRIPKYASRPDKKRIEFRSPDGTCNPYLAMAVMLMAGLDGVRKNMDPTV
                     MGFGPYDM"
BASE COUNT          304 a          391 c          371 g          292 t
ORIGIN      
        1 ttcatatcgt atgggccgaa ccccatgacg gtcggatcca tgttcttcct gaccccgtcc
       61 agaccagcca tcagcatcac ggccatggcc agataagggt tgcacgttcc atcgggcgac
      121 ctgaattcga tgcgcttctt gtccgggcgg ctggcatact tcggaatgcg tatggccgcg
      181 cttcgattac caaggctgaa gaaagcgttg accggggcct cgtaacctgg aacaagccga
      241 cgatatgaat tggtcgtcgg gttcgtaatt gccagtatgg agggagcgtg atgaagcaat
      301 cccgcaacgt acgcaagcgc cgtttttgaa agaccggcat agccgtcctt gtcgaagaac
      361 accggctgcc cgcccttgaa cagatgctgg tggaaatgca tgccgcttcc ggcctctcca
      421 aagaaaggtt tcggcatgaa ggttgcgacc atgccgtgtt ttcttgcggt catcttgaca
      481 aaatacttga ccaggaatgc cacgtcgccc gaacgcatcg gaccggccag atcgacctcg
      541 atttcctgct gcccggtggc gccgacctcg tgatggtgat agttgacgcc aacaccggcc
      601 tgctcgagaa gcacggccat ctcgttcctg atgttaaaga acgcatcggc aggcggacat
      661 gcgtggtatc cgcgatgcgg cgcgatcgta atcctttcgc gggcgccgtc ggccgcagaa
      721 acctggccct cgcgacagac aagcttgtga ctcatttcgg cacaaccggt acgcgtcgcg
      781 acttcgtcaa gcagataaaa ctcgaattcc ggtccgaagc gacagtcgtc cgcaatgcct
      841 gtcgaagcca tgtaatcgca tgcgtgcttc aggatcccgc gcggatccct gggaaacgga
      901 tccaggcttc cagcttcgta aaccccgcat atcagggaga gtgtcttttc cgaaaagaaa
      961 ggatctatga acgccgtcgt gatgtcggga acgataacca tgtccccggc ctcgaccgtc
     1021 ttgaatcctg gcatgttcga tccgtcgaag ccaatcccgc tttcccagat ggactcgtcc
     1081 atcttgcggg caggcagggt gacgtgatgg gcctttccca tcagggcggt gaacttgaaa
     1141 tcgaccgtcg tgacgccttc cttcctgaca tacgcaataa gttcgcgaat cgtcgaaaac
     1201 attttcctct ccaaaagggg taagggatcc gatcgtcaac ctgaaaacac tgtgatgtca
     1261 agaaactgaa ttgtaattgc ttcacaaccc gcgaccgcgg gacgaatcat ggaagcttac
     1321 cctcgatgat cctggcgaca ggtttcaacg tgtcgatg
//
LOCUS       DAOPPA010000637         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_105975_length_1351_cov_2.108796, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000637 DAOPPA010000000
VERSION     DAOPPA010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105975_length_1351_cov_2.108796"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKH54_13805"
     CDS             <1..675
                     /locus_tag="PKH54_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD01018.1"
                     /translation="RAGLAAAGDFEPARGPDIKILLSQVLVAVALLLALACFATSEKL
                     RRLRPLLLISNVLILGVLGGTMLSTALFASWIAGGPNLGRPLLVIAALMSIVLPFVTG
                     RQFWCAFACPFGSACELASRIPARRVPLPRAARAALDIAGSIFLLSIPALLILKPTFD
                     LSLLEPFPAFSLLAAPLVPVVIAAVFLVASVFVPKVWCRFLCPTGRVVNFFTTGGKIF
                     DPPVKN"
     gene            916..>1351
                     /locus_tag="PKH54_13810"
     CDS             916..>1351
                     /locus_tag="PKH54_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01019.1"
                     /translation="MGADALFQPPPLMLASVIDLDLSFFIQLGLFLLLYVLLSRFFFQ
                     PYARILKVRDESTRGMREEAAELEARAAVLEQSTASRLEEARKAGNDERRRIAAEGSR
                     IRAELIANERGRLQAVIDAQVRAIEDKAAAFNAASEPAVADLA"
BASE COUNT          235 a          441 c          374 g          301 t
ORIGIN      
        1 cgggccgggc tggccgcggc cggcgacttc gagccggcaa ggggcccgga catcaaaatt
       61 cttctgtccc aggtgcttgt ggccgtcgcc cttcttctgg ccctcgcatg cttcgcgact
      121 tcggaaaagc tccgcaggct tcgacccctt ctgctgattt caaacgtgct gatactcggg
      181 gttctggggg gcaccatgct ttcaaccgcc cttttcgcgt cctggattgc gggcggaccc
      241 aaccttggaa gacctctgct ggtgatcgcg gcgctgatgt cgatcgtgct gcccttcgtg
      301 accggccggc agttctggtg cgccttcgcg tgtccattcg gatcggcatg cgaactggcc
      361 tcaaggattc cggcgcgtcg cgtgcccctt ccgcgagccg cgagggccgc acttgacatc
      421 gcgggctcga tcttccttct ttcgattcca gcactactga ttctgaagcc gactttcgac
      481 ctttccctcc tggagccatt ccccgcgttt tcgctgcttg cggctccgct tgtcccggtc
      541 gttatcgccg ccgtcttcct tgtcgcgtcc gtcttcgtgc ccaaagtctg gtgccgattc
      601 ctttgcccga cgggacgcgt tgtgaacttt tttacaacag gcggaaaaat cttcgacccg
      661 ccggtgaaga attgacgcaa ccatatttcg ccaataaaac caaataatta tcgcgcctgt
      721 cattttctcc gcgtgccaac gccgcccccg gaagcaaccg ttatttcgac cctgaattgc
      781 cgaaaattga atttccctga tgacatgcgc gccgagtcaa tccataattc cattggccgc
      841 ggattacccc gttcgcgcca cgcggtccgg ccaggcccgg ataatggcag gagacaccag
      901 gaggttcgtt tcgacatggg cgccgacgcc ttgttccaac cgccaccgct gatgctggct
      961 tcggtcatcg acctggatct cagcttcttc atccagcttg gcctgttcct gcttctttat
     1021 gtgcttctgt cccgtttctt cttccagcct tacgcgcgaa tcctgaaggt gcgcgacgaa
     1081 agcaccaggg gcatgcggga agaggccgcc gaactggaag cccgcgccgc cgttctcgaa
     1141 caatcgaccg catccaggct cgaggaggca agaaaggccg gaaacgacga aagacgccgc
     1201 atcgcggccg aagggtcacg catccgcgcc gaattgattg caaacgagcg tggccgcctt
     1261 caggccgtaa tcgatgctca ggtcagggca atcgaagaca aggctgccgc cttcaacgcg
     1321 gcaagcgaac cggcagtcgc cgatcttgcg g
//
LOCUS       DAOPPA010000638         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_106379_length_1347_cov_3.836687, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000638 DAOPPA010000000
VERSION     DAOPPA010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106379_length_1347_cov_3.836687"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..>1347
                     /locus_tag="PKH54_13815"
     CDS             326..>1347
                     /locus_tag="PKH54_13815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD01020.1"
                     /translation="MAGAFRAAVIRAIPWLLFGSVLLPAAGSAVAGILVLVFWRQAGD
                     IALGVLVIVMGLAVIAGTFTSLGLLLRQNRMARAQADFIAFVSHELRTPMASIRMGIE
                     TLRDERFHSEDERREFLQMLEIESRRLSSLVEKAVGYELSADRESGKAARNAATSGCA
                     SELVKNALAGILASPATKDSVVLLAAHDIKGPCNDNVSVDFDAFAGAVVNMVSNALTH
                     GRANHEKPVRVGIEVFFPGVRLHEVLAPFGEVVISVADQGPGVASDETSMIFDRFCRG
                     KAASQTDVPGLGLGLSIVRGFARASGGIVTVEDTPGGGATFRLTLPAHQADSGKTVDC
                     QGHGEH"
BASE COUNT          243 a          409 c          424 g          271 t
ORIGIN      
        1 aaagacgaca agaagttgaa attcagtagg gcccacgcgt tgcaggctgc ccccgtactc
       61 agtcgtgtca atgctatcac cgtcaggcac cagcggtcaa cgtcaccgcc ccgggcgaat
      121 tttcattcgc ccgtacgcct tgtatgggcg gattacatcc gcccgcagcg cctggcacga
      181 ctgtccctgt atgggcggat tacatccgcc cgcagcggtt cagcactcct gccccaatcc
      241 ctgaagctcc gcactacggc atttccgccg gcccggttac aattgcatga catcggtgcg
      301 ggatgattgc tggcgggggt atggcatggc cggtgctttc agggctgccg tcataagggc
      361 gattccgtgg ctgcttttcg gatccgtgct gcttccagcc gccggatccg cggtggcagg
      421 catacttgtc cttgtgttct ggcgccaggc cggggacatc gcgcttggcg tccttgtcat
      481 cgtgatgggt cttgccgtca tcgccggcac gttcaccagc ctggggcttc ttcttcgtca
      541 gaacaggatg gcgcgggccc aggccgactt catcgcgttc gtcagccatg aactgcgcac
      601 cccgatggca tccatccgga tgggcatcga aaccctgagg gacgaaaggt tccattcgga
      661 agacgaacgc cgcgaatttc tgcagatgct ggagatcgaa tcgcgccgtc tttcgtcgct
      721 ggtggaaaag gcggtcggtt acgaactgtc ggcagaccgg gaatcgggaa aggccgcccg
      781 caacgcggcg acaagcgggt gcgcatcgga acttgtcaaa aacgcgctgg ccggaatcct
      841 ggcgtccccg gccacaaagg attcggtcgt ccttcttgcg gcgcatgaca tcaaaggccc
      901 atgcaatgac aacgtatcgg tcgatttcga cgcgtttgcc ggggcagtcg tcaacatggt
      961 ctcaaatgcc cttacgcacg gacgggcgaa tcacgaaaag ccggtgcgcg tcggaatcga
     1021 ggttttcttt cccggtgtcc ggcttcacga agtccttgcg ccgttcggcg aggtcgtcat
     1081 ttcggttgcc gaccagggcc ccggggtggc gtctgacgag accagcatga ttttcgaccg
     1141 cttctgccgc ggaaaggccg caagccagac cgacgttccc ggactggggc tcggcctgtc
     1201 gatagttcgc ggattcgccc gggcgtccgg cgggattgtg accgttgaag acactccggg
     1261 gggcggggca acgttcaggt tgacgcttcc ggcgcaccag gccgacagcg gcaaaaccgt
     1321 tgattgccag ggccatggtg aacatga
//
LOCUS       DAOPPA010000639         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_108072_length_1332_cov_3.396241, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000639 DAOPPA010000000
VERSION     DAOPPA010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108072_length_1332_cov_3.396241"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKH54_13820"
     CDS             <1..830
                     /locus_tag="PKH54_13820"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOD01021.1"
                     /translation="RLAVAVRAPSFGVRTRLVVGQTDLDLNMNLRLLGTLRYPEIWDR
                     IEVVSGGSFTYDVVRRDFEVTRGVVDFDGDVTRPQIDLQAKTRIEYKSGSGSTASASR
                     FNADDNQDSFYSDMIQVNLAVSGTYPELDIQVTSPTRGLSQNDLQVLLLTGIAPGDSI
                     GESGGSFVNVNLLTGSVTDSLVKVLLSDLVDSVSFGVSAGGDVNLDVSAHMGRRLKVQ
                     TQVQQGSGTAQYSTGFKIMLTEGLSLEGRLRAVEYSADQEDVGRNYDTKLRYRIPID"
     gene            889..>1332
                     /locus_tag="PKH54_13825"
     CDS             889..>1332
                     /locus_tag="PKH54_13825"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOD01022.1"
                     /translation="MALLSVPAAASEYEMCGLGPVRSVSLSGCDVTQCSEAGFVSQVL
                     GVGDFRVGGYLVPEMVPVGLERIRKLGFFTMADVECVYDGDGVDVTVEVRPAIRIASI
                     KFKGNKHFWRSDLSEKLSLRVGDAFDLDDKFNVEQLNSAKNSIEKE"
BASE COUNT          283 a          321 c          427 g          301 t
ORIGIN      
        1 ccaggttggc ggtagcggtc cgggcaccgt cgttcggtgt caggacaagg ctggtcgtcg
       61 gccagacgga tcttgatctg aacatgaacc tgaggttgct gggaacactt cgttatcccg
      121 agatctggga tcgcatcgag gttgtcagtg gtggatcgtt cacgtatgac gtggttcggc
      181 gtgatttcga ggtgaccagg ggcgtggtgg atttcgacgg ggatgtcaca cgcccgcaga
      241 tcgacctgca ggcaaagacg cgcatcgagt acaagtccgg ttcaggcagc accgcatcgg
      301 catccaggtt caatgccgac gacaaccagg acagcttcta cagcgacatg atccaggtca
      361 atctggcggt ttcgggaacg tatccggaac tcgacattca ggttacatcc cccaccaggg
      421 gattgagtca gaatgacctt caggttcttc ttttgacggg cattgcccca ggtgattcca
      481 tcggggaaag cggcggttcg tttgtgaacg tgaatcttct taccgggagc gtgaccgatt
      541 cgcttgtgaa ggttcttctg tccgatctgg tcgattcggt gagtttcggt gtcagtgcgg
      601 gcggggatgt caacctggac gtgtcggcac atatggggcg caggttgaag gttcagaccc
      661 aggtccagca gggatccggc accgcccagt acagcaccgg cttcaagata atgcttaccg
      721 aagggctttc gctggaaggt cgcctcaggg cggtcgagta ttcggccgac caggaagacg
      781 tgggaaggaa ctacgatacc aaactcagat acaggattcc gattgactga tcgatcagcc
      841 agagccggtt tgaaactcgg cgcaccgttg atgtcggtga tggtattgat ggcgttgctg
      901 tccgtgccgg cggccgcatc ggaatacgag atgtgcgggc ttggtccggt aagatccgta
      961 agcctgtccg gttgtgacgt tacccagtgt tccgaggccg gttttgtgtc ccaggtgctt
     1021 ggtgttggcg atttcagggt tggcggatac ctggttcccg agatggttcc ggtcgggctg
     1081 gaacgaatca ggaagctggg attcttcacc atggctgacg tcgaatgcgt gtacgacggc
     1141 gatggcgtcg atgtgacggt cgaagtcagg ccagccataa gaatagcaag cataaagttc
     1201 aagggaaaca aacacttctg gcgttcggat ctgtcagaaa aattgtccct cagggttggt
     1261 gacgcctttg atctggacga caagttcaac gtcgagcagc ttaacagcgc gaagaattcg
     1321 atcgaaaagg aa
//
LOCUS       DAOPPA010000640         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_108196_length_1331_cov_3.173197, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000640 DAOPPA010000000
VERSION     DAOPPA010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108196_length_1331_cov_3.173197"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1331)
                     /locus_tag="PKH54_13830"
     CDS             complement(<1..>1331)
                     /locus_tag="PKH54_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01023.1"
                     /translation="TVKEKCPNPTSYSLWYKLERDQGRFGWARASHVAVWNSRHALRP
                     STQAGAELVPGYCDEREAENSARGKSDPCMTFNRTILQAQGERAPFPVIDVKEFGDGV
                     EFDMRKYFKVLVPTLYSKIVATPGSTARDKVMKAEFIILVDATKSMEQEIEGTKNALV
                     QLVRDLKTKYRVDAKFVVLGFRDTDGSSAECPPMEGTTNAQGRLDFVSGEEAIEFLSS
                     LSADCGGDPREAIWDAFYLLRDLVVEPGAQRILFLIGDTPSVEVTRGMTFGGVTVPRG
                     LRSEQVLSKLADVFGESTRFNALLVKSGLMKKTVNEIMKGSRFFANNMQRVDQVSAKI
                     IQAKVSDLFNVAAGSSGADVQAVSDCNNKFLSDDTSGAFGFFCSRKGNNLDSRIKDLL
                     SEEGRTGDVVVIRELWVPATQALQDVALLTIDEARTMGQNLAQLSVQIGED"
BASE COUNT          256 a          394 c          396 g          285 t
ORIGIN      
        1 gtcctcgccg atctggacgg aaagctgggc aaggttctgg cccatcgtcc tggcctcgtc
       61 gattgtcaga agggccacgt cctgcaatgc ctgtgtcgcg ggaacccaca gttcacggat
      121 gacgacgaca tcgccggtcc ggccttcctc agacagaagg tccttgattc ggctgtcgag
      181 attgtttccc ttccttgaac agaagaagcc gaacgcaccg gaagtgtcgt cggaaaggaa
      241 cttgttgttg cagtcgctga ccgcctggac gtcggcgccg gacgagccgg cagccacgtt
      301 gaaaagatca ctgacctttg cctggatgat cttggcgctt acctgatcaa cgcgctgcat
      361 gttgttggca aagaagcggc ttcccttcat gatttcgtta accgtcttct tcataagccc
      421 gctcttgaca agcagggcgt taaacctggt cgattcgccg aacacgtccg ccagctttga
      481 aagaacctgt tccgaacgca ggccgcgcgg cacagtgacg ccgccgaagg tcatgccgcg
      541 ggtcacttca acactgggtg tgtcgccaat caggaaaagg attcgctggg caccgggctc
      601 gaccaccagg tcgcgcaaca ggtaaaacgc gtcccagatt gcctcgcgcg gatcgccgcc
      661 acagtcggcc gacaggctgg acaggaattc aatcgcttcc tctccggaaa cgaagtccag
      721 ccgcccttgg gcgttggtgg tgccttccat cggggggcat tcggccgaag agccgtcggt
      781 gtcgcggaag cccaggacaa cgaacttcgc atcgacccgg tacttggttt tcagatcgcg
      841 cacaagttga accagggcgt tcttggtgcc ttcgatttcc tgttccatcg acttggtcgc
      901 atcgaccagg atgatgaatt cggccttcat gaccttgtct ctggccgtac ttccgggtgt
      961 cgcgacaatc ttgctgtaaa gcgtgggaac cagaaccttg aagtacttcc tcatgtcgaa
     1021 ttcaacgcca tcgccgaatt ccttgacgtc gatgaccggg aacggggcac gttcgccctg
     1081 ggcctgcaga atggtgcgat tgaaggtcat gcaggggtcg ctcttgccgc gggccgaatt
     1141 ttcggcctcg cgctcgtcgc agtatccggg gacgagttcg gcgcctgcct gggtggatgg
     1201 acgcagcgca tggcggctgt tccagacggc gacgtggctg gcgcgtgccc agccgaaacg
     1261 cccctgatca cgttcaagct tgtaccacag ggaatacgac gtcggattgg gacacttttc
     1321 cttgacggtt c
//
LOCUS       DAOPPA010000641         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_108657_length_1327_cov_3.318396, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000641 DAOPPA010000000
VERSION     DAOPPA010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108657_length_1327_cov_3.318396"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1116)
                     /locus_tag="PKH54_13835"
     CDS             complement(160..1116)
                     /locus_tag="PKH54_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD01024.1"
                     /translation="MSCLYMSLALFVVLSAFSCSRTPDPGTAAQSSQPDRTAMAIEPG
                     QVETASQRSGTGRDPRKPGLIDSPAPSGMVLVPGGRFLMGCSDGHDGVVDDKCAPSEF
                     PARSVHVDPFFIDIDEVTVGQYTDCVNAGVCQAPYSGGECNFGEKDRMRYPINCVTWD
                     DARLFCKWAGKRLPYEAEWEKAARGTDGRRYPWGNQEPDEGGSWRANLGEGLTKLLWM
                     RDQWEYDAPVGFFSGYASPHGCNDMAGNLAEWVHDWWAESYAGMPDTNPRGPDVGNQH
                     VVRGGSFREYRQRIRTSARGYHYTDFMDGNVGFRCAADAGHE"
BASE COUNT          279 a          432 c          356 g          260 t
ORIGIN      
        1 ggaaagtttc ggcttccaac acggcggttg ccccttcctg cgacaagcca ggcgccgaag
       61 gtttccgccc tggcacggct gacgggaccc catcgcgcaa ccgaagctcc tggagcttgg
      121 cgttcagaag ctgcgcgatc ctgtcacgat ccttgccgct cattcgtgcc ccgcatccgc
      181 cgcacacctg aaacccacgt tgccgtccat gaaatcggtg tagtggtagc cacgcgccga
      241 cgtcctgatc cgctggcgat attctcggaa cgaaccgcca cggacgacgt gctggtttcc
      301 gacatccggc ccgcgaggat tggtgtcagg catccccgca taagactcgg cccaccagtc
      361 gtggacccat tcggccaggt ttcctgccat gtcattgcag ccatgcggcg atgcatatcc
      421 ggaaaagaaa ccgaccgggg cgtcatattc ccactggtcg cgcatccaca acagtttcgt
      481 aagcccttca cccagatttg cccgccagga tccgccttcg tccggttcct gatttcccca
      541 cggatatcgc cttccgtcag tgccacgggc ggccttttcc cattccgcct catagggcag
      601 gcgcttgccg gcccatttgc agaagaggcg tgcgtcatcc caggtgacgc aattaatcgg
      661 atacctcatc cggtcctttt ccccaaaatt gcattcgcca ccactgtacg gagcctggca
      721 gacaccggca ttcacgcagt cagtgtattg tccgaccgtc acctcgtcga tatcgatgaa
      781 gaaaggatcg acgtgtaccg accgggccgg aaactcggat ggcgcgcact tgtcatcaac
      841 cacgccgtca tgtccgtcgg aacaccccat aaggaaccga ccacctggca ccaggaccat
      901 gccggatggc gctggactgt ctattagccc aggcttccgt gggtcacggc ctgtgccgga
      961 acgctggctt gcagtttcaa cctgaccagg ctcaatggcc atggcagttc gatccggctg
     1021 tgacgactgg gccgcggttc ccggatccgg ggttcttgaa caggagaatg cggacaggac
     1081 aacgaacagc gccagggaca tgtacaggca agacattgaa acctccggaa aaaccggctt
     1141 ctgattgtgg acacctgtca ccggcgattg tacatcgccg acaggtcgaa ttcacatgat
     1201 caccaccggt cgaacaaacc gaaccgtttt atcctgtata atccggcccc ggaatgaagg
     1261 ggacgcacgc tttgaccaac ggcacgattc gaaaaattcc agaagtactc gcgcccggtg
     1321 gatccat
//
LOCUS       DAOPPA010000642         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_110650_length_1310_cov_4.425498, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000642 DAOPPA010000000
VERSION     DAOPPA010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110650_length_1310_cov_4.425498"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1089)
                     /locus_tag="PKH54_13840"
     CDS             complement(127..1089)
                     /locus_tag="PKH54_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOD01025.1"
                     /translation="MTDLMVEGLPLWFFKVFALLIGAAFGSFANVVIYRVPLGRSVVR
                     PGSSCPGCGHEIAWYENIPIASWLFLRARCSSCGTRISIRYPLIELMTAVLSLACMML
                     GIAAVPGLELTAARLVLLWFFPFALCFLTVCIVFIDFEHWIIPHPLTAAGIVLGIASS
                     VVLAPPLGDVHWYESLIGAAAGALPIIAIIEIYFRLTGREGMGYGDVMLMAMAGAWFG
                     YLALPFILLASSIQGLLGAIPAVVSKNRPVPPWEGAEAAEADATDAEPADATAPDGSA
                     IHVDRPAAEAGELRHLALPFGPFIGLAMIEWLFFGDLVTTWFFG"
     gene            complement(1118..>1310)
                     /locus_tag="PKH54_13845"
     CDS             complement(1118..>1310)
                     /locus_tag="PKH54_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01026.1"
                     /translation="KKGDVIVDTYTLDVPIGSPAGDYDVWMGLYLPGGSRLKVVSFDE
                     DKVRNDGSDRVRIGKMTVR"
BASE COUNT          291 a          398 c          408 g          213 t
ORIGIN      
        1 gatggagact ataccgtcgc gacctgtccc aggggcgaga ttgttcagac ggtcgtcggt
       61 gaagggagtc gtgaaacggt gcgtcagttc attcccgcaa gctgcacgtt ctgaaaatgc
      121 ctcgattcag ccgaagaacc acgtcgtgac cagatcgccg aagaacagcc attcaatcat
      181 ggcaaggccg atgaacgggc cgaatggcag ggcaaggtgt cgcagctccc ctgcctccgc
      241 cgcgggccga tccacgtgga tggccgaccc gtccggggcg gttgcgtcag cgggctctgc
      301 gtcagttgcg tcggcctcgg cggcctcggc gccctcccat ggcgggacgg ggcggttttt
      361 tgaaacgaca gccggaatcg cgccaagaag accttgaatc gaactggcca ggaggatgaa
      421 gggcagggca aggtaaccga accacgcgcc ggccatcgcc atcagcatca cgtccccgta
      481 acccatgccc tcgcgcccgg tcaggcggaa gtaaatctcg attatggcaa ttatgggcaa
      541 tgcgccggcc gccgcgccga tcagggattc gtaccagtgg acgtcgccca ggggcggcgc
      601 gagcacgacc gaggacgcga tgcccaggac gatgcctgcc gcggtaagcg ggtgcgggat
      661 gatccagtgt tcgaaatcga tgaagacgat gcatacggtc aggaagcaca gcgcgaacgg
      721 gaagaaccaa agaagtacaa gccgggccgc cgtcagttcc agtcccggca cggcggcaat
      781 cccgagcatc atgcatgcaa gggacagaac cgccgtcatc agctcaatca agggataccg
      841 gatggaaatt ctggtgccgc aggaactgca ccttgcccga aggaacaacc agcttgcaat
      901 cgggatgttt tcgtaccagg cgatctcgtg gccgcagccg gggcaggaag agccaggcct
      961 gacgaccgat cttccgagtg gcaccctgta gatgacgacg ttggcgaacg agccgaaggc
     1021 ggccccgatc agaagggcga aaaccttgaa gaaccacagt ggcagcccct caaccatcaa
     1081 gtccgtcatg tgccgccccc ggaataggcc ggaaatacta cctgaccgtc atctttccga
     1141 ttcttaccct gtccgatccg tcgttccgga ccttgtcctc gtcgaaggaa accaccttca
     1201 ggcggctgcc gccgggaagg tagagtccca tccagacgtc ataatcgccg gccggagagc
     1261 cgatcggaac gtccagggta taggtgtcga cgattacgtc gccctttttc
//
LOCUS       DAOPPA010000643         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_111611_length_1302_cov_3.287089, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000643 DAOPPA010000000
VERSION     DAOPPA010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111611_length_1302_cov_3.287089"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..837
                     /gene="mazG"
                     /locus_tag="PKH54_13850"
     CDS             37..837
                     /gene="mazG"
                     /locus_tag="PKH54_13850"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOD01027.1"
                     /translation="MSEKDTRTGQAFIDAVGIMRRLRAPDGCPWDREQSLKTLKAYVI
                     EEAFEVVEAIDEGRPELLREELGDLLLQVLFQAQIMDEQGVFDAADVCRALVDKLVAR
                     HPHVFGDVDNNGSTGDVLRRWESFKKKAGRGLLDGVPRNLPALLMAMRISDKAGNVGF
                     EWPDPSGALDKLEEEIGELRRAFLDGDSQAVEDELGDVLFAAANFARMKKINPEDSLR
                     QALARFRSRFSSMEAALAAQGRGLADADMKQLDLLWEQAKAAERCKAE"
     gene            884..>1302
                     /locus_tag="PKH54_13855"
     CDS             884..>1302
                     /locus_tag="PKH54_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOD01028.1"
                     /translation="MVPGVAEETANQGIPKPSGGNSRASILKSSLLMSVMTALSRLFG
                     LLREQVQGYFLGTGMGADAFAIGSTIPNLLRRLFAEGQMTAAFVPVLKDEMVFGDRER
                     LTRFYSGFMTLFILLMLAVTLLGIAFAEPIVRYIFAS"
BASE COUNT          247 a          369 c          410 g          276 t
ORIGIN      
        1 ccgccgcttc atttttcgga acaaggatta ttcataatga gcgaaaaaga cactcgtacg
       61 ggacaggcgt tcatcgacgc cgtcgggatc atgcgccgcc ttcgggcgcc ggacggttgc
      121 ccgtgggatc gcgaacagtc gctgaaaacg ctgaaggcgt atgtgatcga ggaagccttc
      181 gaggtcgtcg aggccattga cgaggggcgc ccggaacttc tgcgcgagga acttggggat
      241 ctgcttcttc aggtactctt ccaggcccag attatggacg aacagggcgt ttttgacgct
      301 gccgacgtct gccgcgcgct tgtcgacaag ctggtggcac gccatcccca cgttttcggg
      361 gatgtcgata acaacggcag caccggcgat gtcctgcgtc ggtgggaaag tttcaagaag
      421 aaggccgggc gtggtctgct tgacggcgtg ccaaggaatc ttccggcgct gctgatggcc
      481 atgaggattt ccgacaaggc tggcaacgtc gggttcgaat ggcccgaccc gtctggcgcc
      541 ctggacaagc ttgaagagga gatcggcgaa cttcggcggg cattcctgga cggcgattca
      601 caggctgtcg aggatgagtt gggcgacgtg ctttttgccg ccgcgaattt cgcgcggatg
      661 aagaagatta atccggaaga cagcctgcgt caggccctgg ccaggttccg ttcgcgattc
      721 tcgtcgatgg aagccgccct tgccgcccag ggacgcgggc tggccgacgc cgacatgaag
      781 cagcttgacc ttctttggga acaggccaag gccgccgagc gctgcaaggc ggagtaatct
      841 ggcgcgtccg catcatccgg cggataatcg gcaggtcagt tgaatggttc ccggcgtggc
      901 tgaagaaacc gcaaatcagg gcatccccaa gccgtccggc ggcaactcca gggcttcaat
      961 actcaaatca agcctgctga tgtccgtgat gacggccctt tccaggcttt tcgggctgct
     1021 gcgcgagcag gtccagggct acttccttgg aaccggaatg ggtgccgatg ccttcgcgat
     1081 cggttcaacc atcccgaatc ttcttcgccg cctttttgcg gaaggccaga tgacggcagc
     1141 tttcgtcccg gtgctgaagg acgagatggt tttcggcgac agggaacgcc tgacccggtt
     1201 ctattccggc ttcatgaccc ttttcatcct gttgatgctt gccgtcacgt tgctcgggat
     1261 tgccttcgcc gaaccgatcg tcaggtacat ctttgcatcg aa
//
LOCUS       DAOPPA010000644         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_111660_length_1302_cov_2.072173, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000644 DAOPPA010000000
VERSION     DAOPPA010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111660_length_1302_cov_2.072173"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKH54_13860"
     CDS             <1..741
                     /locus_tag="PKH54_13860"
                     /inference="COORDINATES: protein motif:HMM:NF025693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HOD01029.1"
                     /translation="STRVIGRTVFRQNAALPPADARIIESSMTDMKVVATIRPAEFMI
                     PAYVDDVREYLEILLVRASVKAEPAADKVAGVLHRAMPYPVVVVLDMPGDAVFLSVAH
                     KRNPVNNQGRMVVESMNSVVLSGRGGSDGPERAFLASLDVSRLPARNLFVTYQAVWDR
                     ITAMRAASVVNVFGIPPDPDQAALLERTLSEYLKLTDQADALRSRAERETQMNRRVEY
                     NDQISKLGPRQIAAVLKMQAVMAAGVGK"
     gene            747..>1302
                     /locus_tag="PKH54_13865"
     CDS             747..>1302
                     /locus_tag="PKH54_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD01030.1"
                     /translation="MKRIDADDELGRSADVASENLEQLRTLFPEAFTEGKLDLQVLRQ
                     LVGDLVVDEATDGKMPDEKYGLNWKGKRRARQLALTPSLGTLRPCPEESVDWDTTKNL
                     MIEGDNLEVLKLLQKSYSGKVKLIYIDPPYNTGKDFVYPDNFHDNIRNYLELTGQVDG
                     EGRKLTSNPESSGRFHTDWLNMMYP"
BASE COUNT          293 a          342 c          387 g          280 t
ORIGIN      
        1 agcacccgtg taatcggccg tacggtcttt cgtcagaatg cggcgttgcc gcctgctgat
       61 gccagaatca ttgaatcttc gatgaccgat atgaaagtcg tcgccacaat ccggcctgcg
      121 gaattcatga ttcctgcgta tgtggacgat gttcgcgaat atctggaaat cctgctggtc
      181 cgcgccagcg tgaaggcgga gcccgcagcc gacaaggttg caggcgtgtt gcatcgggcg
      241 atgccatacc cggtggttgt tgtacttgat atgcccggcg atgccgtttt cttgtccgtc
      301 gcccacaagc gaaatcctgt caataatcag ggcagaatgg tggttgagtc catgaattcg
      361 gtggtacttt cgggccgggg cggctctgat ggtcccgaac gcgccttcct ggcatcactg
      421 gacgtttcca gactgccggc acgcaacctg tttgtaacgt accaggcggt ttgggatcgg
      481 attacggcca tgcgtgcggc atcggtcgtg aacgtgtttg gaattccacc ggatccggat
      541 caggccgctt tgttggaaag gacactttct gaatacttga aactgacgga ccaggcggat
      601 gcgcttcgtt cccgtgcaga gcgcgagacc cagatgaacc gcagggttga atacaatgac
      661 cagataagca aacttgggcc gcgtcagatt gcggccgttt tgaagatgca ggctgtcatg
      721 gccgccgggg tgggaaagta atggatatga agcgaatcga tgccgacgat gaactgggcc
      781 gcagcgccga tgtggcttcc gaaaaccttg aacagttacg cacgctcttc cccgaggctt
      841 tcacggaagg gaagcttgat ctgcaggttc ttcgtcagtt ggttggcgac ctggtcgtcg
      901 acgaggcgac tgatggcaag atgcctgatg aaaagtatgg gctgaactgg aagggtaaac
      961 gtcgcgcacg tcagttggcc ctgaccccgt ccctgggaac gctgcgtcct tgcccggaag
     1021 aaagcgttga ttgggacacg accaagaacc tgatgatcga aggggacaat ctggaagtcc
     1081 tgaagctgct tcagaaaagc tattcaggca aggtaaagct gatatacatc gacccgccgt
     1141 ataacaccgg aaaggatttc gtttatccgg acaacttcca cgacaacatc cgcaattacc
     1201 tggaactgac cggtcaggtc gatggcgagg gccgcaagct gaccagcaac cctgaatcca
     1261 gcggccgctt ccacaccgac tggctgaaca tgatgtaccc ca
//
LOCUS       DAOPPA010000645         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_113311_length_1288_cov_4.787510, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000645 DAOPPA010000000
VERSION     DAOPPA010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113311_length_1288_cov_4.787510"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKH54_13870"
     CDS             complement(<1..373)
                     /locus_tag="PKH54_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01031.1"
                     /translation="MTSAIFSGTAYVQIQEEVKLLINNCPDFLSIQTADHPRAVGDAI
                     EELIADNLASILGPLCTEYSSSFARRAMADMAFKGSDGLYYVVDVKTHRTDTRFNMPN
                     LTSVERLSRFYEDDSNCFCIMM"
     gene            complement(370..>1288)
                     /locus_tag="PKH54_13875"
     CDS             complement(370..>1288)
                     /locus_tag="PKH54_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD01032.1"
                     /translation="RLVPAEAVADLESCLNRTILGDCRDVLRRLPAASIDLIVADPPY
                     NMTKTFSKTRFRRQDDDAYAEWLDSWLSLARLVMKETASIYVCSEWRSSGLICQVLER
                     HFMVRNRITWEREKGRGALRNWKNCSEDIWFATVSDDYYFDVDAVKQKRRVVAPYRDA
                     SGAPKDWVDGDDGAYRITHPSNLWTDISVPFWSMPENTDHPTQKPEKLIAKLVLANSR
                     PGDLILDPFAGSGTTAVVAAKLGRRHIGIETELEYCLLGEARLQAAAADQSIQGYSDG
                     YFWDRNTLAEQRKVSRQADDQSSLFEVNE"
BASE COUNT          295 a          364 c          353 g          276 t
ORIGIN      
        1 ccatcatgat gcagaagcag ttcgagtcat cttcgtaaaa cctggacagg cgctccaccg
       61 atgtcaggtt cggcatgttg aacctcgtgt cggttcggtg ggtcttaaca tcgacgacat
      121 agtacaaacc gtccgagccc ttaaaggcca tgtcggccat ggcacggcgg gcaaaacttg
      181 acgagtattc ggtgcaaagc gggcccagaa tgctggccag attatcagcg atcagttcct
      241 cgatcgcgtc cccaacggcg cgggggtgat ccgccgtctg tatggacaga aagtcaggac
      301 agttgtttat caggagcttc acttcttcct gaatctgcac atacgcggtt ccgctgaaga
      361 tcgcgcttgt cattcattga cctcaaaaag cgatgactgg tcgtctgcct gccgggacac
      421 cttacgttgt tcggccaacg tgttccgatc ccagaaatat ccgtcggagt agccctgaat
      481 ggattggtcg gccgcagcgg cctgaaggcg ggcctcgccc agaagacaat actcaagttc
      541 ggtctcgatg ccgatatgcc ggcgtccgag cttggcggcg acaaccgcgg tggtaccgga
      601 accggcaaac ggatcaagga tcagatcgcc agggcgggag ttggccagaa cgagcttggc
      661 aataagcttt tcgggcttct gagtgggatg atccgtattt tcaggcatgg accagaaagg
      721 gaccgaaata tctgtccaca gattcgaagg gtgcgtgatc cgataagcgc catcatcgcc
      781 gtcaacccag tccttgggcg cgccggacgc gtcacggtag ggggcaacga cacgacgctt
      841 ctgcttgacc gcatcgacat caaaataata atcgtcggac acagtggcaa accagatatc
      901 ttcagaacag ttcttccagt ttcgcagggc gccacggccc ttttcgcgtt cccaggtaat
      961 ccggtttctt accatgaaat gcctttcaag aacctgacag atcaagcccg atgatcgcca
     1021 ttcactgcac acgtaaatcg acgccgtttc cttcatgaca agcctggcaa gcgacagcca
     1081 cgaatccagc cattctgcat aggcatcgtc gtcctgacgc ctgaaccggg ttttactgaa
     1141 ggtctttgtc atgttgtacg gtgggtcggc aactatcagg tcaattgacg ccgccggcag
     1201 tctgcgcagg acgtcacggc aatcaccaag gattgtccgg ttcaggcagg actccagatc
     1261 tgcaaccgct tcggctggaa ccaacctc
//
LOCUS       DAOPPA010000646         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_114459_length_1279_cov_3.322712, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000646 DAOPPA010000000
VERSION     DAOPPA010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114459_length_1279_cov_3.322712"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKH54_13880"
     CDS             complement(<1..833)
                     /locus_tag="PKH54_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01033.1"
                     /translation="MRKAIFGLTIAVTLSVGCGSDWIAGYDLIDDAVVADIASDLVPG
                     DSTGDPGVDANRGDVVEDAADNGDVAVDAVGETVSPVDSTEVLAEDAVQTDLNVPFME
                     ERAGWAWNSSPLDGEPTQVVYRHLESRQGKLTGKFADVWNCLREDGGPIEQMYWDGIV
                     YDIQLCHYKQTVVPGPDGTYLHVLPPESARDGNDPFAEGHTYYCVNRVHDFFTDQFGL
                     TDNDRSLQCVVNMGFQVLGSPGWMPLDNAAFLPDASYGFMGFNMGDGELLAFGQGRRI
                     D"
     gene            complement(838..>1279)
                     /locus_tag="PKH54_13885"
     CDS             complement(838..>1279)
                     /locus_tag="PKH54_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01034.1"
                     /translation="LWGDRTISPLETRVESSRFGHQAFMTITLDGAPVIDQVIRIQAD
                     QDGVVQSVSSTLPATFDGLTVIRDSEKVTMAAAMKAICDHAAARYDGLICPKQVQRVW
                     FRDGMTLVPGGRMLLRSADMGSLLEAIVDLRTGEIVRFAELARR"
BASE COUNT          267 a          401 c          352 g          259 t
ORIGIN      
        1 aagtcgatgc ggcgtccctg gccgaacgcc agtagttcac cgtcgcccat gttgaacccc
       61 ataaatccgt atgaagcgtc cggcaggaaa gcggcgttat caagcggcat ccatcccgga
      121 ctgccaagaa cctggaaacc catgttgacc acgcactgca ggctgcggtc gttgtctgta
      181 aggccgaact gatcagtgaa gaaatcgtgg acgcggttca cgcagtagta agtgtggcct
      241 tccgcgaacg gatcgttccc gtccctggcg ctttccgggg gcagcacatg caggtaggtt
      301 ccatccgggc cgggcaccac tgtttgcttg taatggcaaa gctggatatc atagacaatg
      361 ccgtcccaat acatctgctc gatcggcccg ccgtcctcgc gaaggcagtt ccagacatcg
      421 gcgaattttc ccgtcagttt tccctggcgg gattcaaggt gccggtaaac gacctgggtc
      481 ggctccccgt caagcggcga agaattccag gcccacccgg cacgttcttc catgaaggga
      541 acgttcaggt cggtctgtac cgcatcctcg gccaggactt cggttgaatc gacgggcgaa
      601 acggtctcac cgaccgcatc gacggcaaca tccccgttgt cagcggcgtc ctcgacaacg
      661 tcgccgcgat ttgcatcgac cccggggtcg ccggtcgaat cgccgggaac cagatccgat
      721 gcgatgtcgg ccacgacggc gtcatcaatc aaatcgtaac cggcaatcca gtcgcttcca
      781 catccgaccg aaagtgtgac cgcaatcgtc aatccaaata tcgcttttct catcgggtca
      841 cctcctggcc agttcggcga agcgcacaat ttcaccggtt cgcagatcga caatcgcctc
      901 gagcagactt cccatgtcgg cggaacgcaa cagcattctt ccgcccggaa ccagggtcat
      961 gccgtccctg aaccagacgc gctgaacctg tttcgggcag ataagtccgt cataccttgc
     1021 cgccgcatgg tcgcagatgg ccttcatggc agcagccatg gtgacctttt cagaatcacg
     1081 gatgaccgtc agcccatcaa acgtcgccgg aagggtcgag cttaccgact ggacaacccc
     1141 gtcttgatcg gcctggatcc ggatgacctg gtcgatgacc ggggcgccgt ccagtgtgat
     1201 agtcatgaat gcctggtgtc cgaacctcga tgattcaact cttgtttcaa gtggcgatat
     1261 cgtgcggtcg ccccacagt
//
LOCUS       DAOPPA010000647         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_114831_length_1276_cov_5.138411, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000647 DAOPPA010000000
VERSION     DAOPPA010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114831_length_1276_cov_5.138411"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKH54_13890"
     CDS             <1..439
                     /locus_tag="PKH54_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD01035.1"
                     /translation="IVESVMMVCEPRASRKDIRILATVQPDAVAFVNQPLMEQALLNL
                     VENAVKYTDQGGHVDVAASVVDGRLTLSVSDDGCGIPREHLPRIFERFYRVDKGRSRS
                     QGGTGLGLSIVRHVATIHGGKVRVTSEPGVGSQFVIDVPAIQG"
     gene            569..>1276
                     /gene="thiM"
                     /locus_tag="PKH54_13895"
     CDS             569..>1276
                     /gene="thiM"
                     /locus_tag="PKH54_13895"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOD01036.1"
                     /translation="MKQFVDGLVLDMLEIRRRRPLVFSMTNFVVMNETANALLAVGAS
                     PVMSDAIEEAVEMAGISSALVLNIGTLYPRSVDSMVEAGRAANRKGIPVVLDPVGAGA
                     TRLRTDSARRILSEVDVTILRANQGEAMAVAGLESRVRGVDSVDDIGQFTDIAIRIAE
                     GIGCPVAVTGRTDVITDGSQTWLCDNGHPLLQAVTGTGCTVTALVGAFLAVNPDVTRA
                     AAGALGLFGMCGELAAQG"
BASE COUNT          225 a          340 c          453 g          258 t
ORIGIN      
        1 catcgtcgag tcggtgatga tggtctgcga gcccagggcg tcacggaagg acatccggat
       61 tctggcgacg gtgcagccgg atgccgtggc gttcgtcaat cagccgctga tggagcaggc
      121 ccttttgaat cttgtggaga acgccgtcaa atacaccgat cagggcggtc atgtggatgt
      181 ggcggccagc gttgtggacg ggaggctgac gctgtcggtt tcggacgacg gatgcggcat
      241 tccaagagag caccttccca ggatttttga acgattctat cgtgttgaca aggggcgcag
      301 cagatcgcag ggcggaaccg gcctggggct ctcaatcgtg cgccatgtcg cgacgattca
      361 cggcggcaag gtcagggtca ccagcgagcc cggggtcggg agccagttcg ttatcgatgt
      421 tcccgccatc cagggttgag cggcagccgg ccgaatcttt tccagccgcg acttcatggg
      481 ttatgcttcg tgccttcgga tggtcggtcg gccgtccgga aattgcagtc agtgtggcaa
      541 aacgtccgtg tcaggtcatg gtgaagcaat gaaacagttt gtcgatgggc ttgttctgga
      601 catgctcgag attcgcaggc gaaggccgct tgtgttcagc atgaccaatt tcgtggtgat
      661 gaacgagacg gccaacgccc tgctcgcggt cggggcgtcg ccggtcatgt ccgatgcaat
      721 cgaggaagcg gtcgagatgg ccggcatatc cagcgcgctg gtgctgaaca tcggaacgct
      781 gtatccgcgg tcggtcgatt ccatggtcga ggcgggcagg gctgccaaca ggaaggggat
      841 tccggtcgtg ctcgatccgg tgggggcggg tgcgacaagg ttgcgcaccg attcggcccg
      901 caggatcctg tcggaagtcg atgtgaccat ccttcgggcg aaccagggcg aggcgatggc
      961 ggtggccggt ctcgaatcga gggtgcgtgg ggtcgattcc gtcgatgaca tcggtcaatt
     1021 cacggatatt gcgattcgga ttgccgaagg catcggatgt ccggtggcgg taacaggccg
     1081 cacggatgtg ataacggacg gcagccagac ctggctttgc gacaatggac atccattgct
     1141 tcaggccgtc accggaaccg gttgcacggt taccgcgctg gtcggggcct tcctggcggt
     1201 caacccggac gtgacgcgcg ccgcggccgg ggcccttggc cttttcggca tgtgcggaga
     1261 gcttgcggcc cagggt
//
LOCUS       DAOPPA010000648         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_114840_length_1276_cov_4.201474, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000648 DAOPPA010000000
VERSION     DAOPPA010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114840_length_1276_cov_4.201474"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1276)
                     /locus_tag="PKH54_13900"
     CDS             complement(<1..>1276)
                     /locus_tag="PKH54_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD01037.1"
                     /translation="FPRYDLDMSLLRAYENGKPARTPEFFKIDPKGAADGELTFVSGH
                     PGRTQRLLTVAELEFLRDVAFPSRLMRLSEARGMLAQFGRQGPEAKRISQEGLFGVEN
                     AYKAIGGEWRALLDKTRFQQKIDAENALRQTVTDPEKAGAWDEIARAQRNFTEFYDAY
                     NMLERGQAFPSDLFQIARMLVRGVIEREKPDAERLREFTDAALPTLQQYLFSTAPIYP
                     DIEEAWMTFGLTKFREILTTGHPDVIKVLGRESPESLARALVRESKLADGEVRKALWD
                     GGIMAVLDSSDPMIKLALAVDDSARALRARYETEVEAVVRRNSEIIARAHFDKAAGKT
                     YPDATFTLRLSYGTVKGYTDHGRQVVPMTTIAGAFERHTGQDPFALPKSWLDTRESLK
                     PDTQFNIATTNDIIGGNSGSPVFDPQTGEVIGV"
BASE COUNT          223 a          385 c          389 g          279 t
ORIGIN      
        1 acgccgatca cttcacccgt ctgcgggtcg aacaccgggc tgccgctgtt gccgccaatg
       61 atgtcgttcg tggtggcgat gttgaactgg gtatccggct taaggctctc gcgcgtgtcc
      121 agccacgatt tcggcagggc aaacggatcc tggccggtat ggcgctcgaa ggctccggcg
      181 atggtcgtca tggggacaac ctgtcggcca tggtcggtgt agcccttcac ggtgccgtac
      241 gacaggcgca gggtgaaggt cgcgtccggg tatgtctttc cggccgcctt gtcgaaatgg
      301 gcacgcgcaa tgatttcgct gttcctgcgc acaaccgcct cgacctcggt ttcgtatctg
      361 gcgcgcaggg cccttgccga gtcatcgacg gccagggcca gcttgatcat tggatcactt
      421 gaatccagta ccgccatgat gccgccatcc cataaagcct tgcggacttc gccatcggcc
      481 agtttcgatt cgcgcacgag agcccttgcc aggctttcgg gtgactcgcg gccaagcacc
      541 ttgattacgt ccgggtgacc tgtcgtcagg atttcacgaa acttcgtaag accgaaggtc
      601 atccaggcct cttcaatgtc gggatagatc ggcgccgtgc tgaaaaggta ctgctgaagc
      661 gttggcagtg cggcgtccgt gaactcccgc agacgctcgg cgtccggctt ttcgcgctcg
      721 atcacgccac ggaccagcat ccttgcaatc tggaacagat cggacgggaa tgcctggccg
      781 cgttcaagca tgttgtaggc gtcgtagaat tcggtgaaat tccgctgtgc cctggcgatt
      841 tcgtcccacg cgccggcctt ttcgggatcg gttacggtct gtcgcagggc gttttccgca
      901 tcgattttct gctgaaagcg ggtcttgtcc aaaagggccc gccactcgcc tccgattgcc
      961 ttgtaagcat tttccactcc gaacaggcct tcctggctga ttcgttttgc ctcggggccc
     1021 tgacgtccga actgggccag catgccgcgc gcctcggaaa ggcgcatcag gcgggacggg
     1081 aaagcgacgt cgcgaaggaa ttcaagttca gctacggtaa gaagccgctg ggtgcgtcca
     1141 gggtgtcccg aaacgaaggt caattcgcca tcggccgcgc ccttcgggtc gatcttgaag
     1201 aactcgggcg tccttgccgg ctttccattc tcgtacgcac gaagcaggga catgtccagg
     1261 tcatacctgg ggaacg
//
LOCUS       DAOPPA010000649         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_115139_length_1274_cov_3.051682, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000649 DAOPPA010000000
VERSION     DAOPPA010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115139_length_1274_cov_3.051682"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1274
                     /locus_tag="PKH54_13905"
     CDS             32..>1274
                     /locus_tag="PKH54_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOD01038.1"
                     /translation="MYHQYIPGEYVIDRMNWGHATSTDLVRWEYRPVAISTNPELGMA
                     YSGSAIADPDNTTGLCTGDPADCILAFFTHSLMLGGDQKQSLAASSDGGLTFVEYGDN
                     PIMANPGKTNFRDPKVFMFDESQARARASHIGPVATQWNMVLAQGKQIGLYVSGNLIE
                     WQPLSTFDNNPDWTGGVWECPDLFRMPVQDEPGAFKWVLITSVFDGAPWDGSGVMYFT
                     GDFDGTTFTPDDPDLMPRWLDHGPDFYAAQTFANVPDSDGRRILIAWMNNWKYAMGIP
                     AMPWQGAMAIPRQLNLVRDGDGTLTLTQAPVSEFKSLRRGRVVELTDITSGSAALPVH
                     SSDTGMYEIDITAPRWDKWSIGIASMTALMQPDGAEMDPMPITENARVEIGIDNGILS
                     LDRTGAICDECRQRSLGVALRR"
BASE COUNT          257 a          396 c          401 g          220 t
ORIGIN      
        1 cggcctgata ttgcaccgcg gaacctggca catgtaccac cagtatattc cgggcgagta
       61 cgtcatcgac cggatgaact ggggccatgc cacaagcacg gatctggtcc gctgggaata
      121 ccggccggtc gccatatcca cgaaccccga actcggcatg gcatattcgg ggtcggccat
      181 cgcggatccg gacaacacaa ccggcctttg caccggcgac ccggccgact gcatactggc
      241 ctttttcacg cacagcctga tgctgggcgg cgaccagaag cagagcctgg cggcaagcag
      301 cgacggcggc ctgacgttcg tggaatacgg cgacaacccg atcatggcga atcccggcaa
      361 gaccaatttc cgtgacccga aggtattcat gtttgacgaa tcgcaggcca gagcgcgcgc
      421 aagccacatc gggcctgtcg cgacgcaatg gaacatggtg ctggcccagg gcaaacagat
      481 tggcctttat gtatcgggga acctgatcga atggcagccc ctttcgacgt tcgacaacaa
      541 cccggactgg acaggcggag tctgggagtg tccggattta ttcaggatgc cggttcagga
      601 cgagcccggc gctttcaagt gggttctgat tacaagcgtg ttcgacgggg cgccctggga
      661 cggctccggc gtcatgtact tcaccggtga tttcgacggg accacgttca cgcccgatga
      721 cccggatctt atgccgcgct ggctggatca cggaccggac ttctacgcgg cgcagacgtt
      781 tgcgaacgtg cccgacagcg acgggcggcg catcctgatt gcctggatga acaactggaa
      841 gtacgcgatg ggcatccccg cgatgccgtg gcagggcgcg atggcgattc ccaggcagtt
      901 gaacctggtc agggacggcg acgggaccct gacactgacc caggcgcccg tgagcgagtt
      961 caagtcgctg cgcagggggc gcgtcgtcga attgacggac atcacctccg gatcggccgc
     1021 cctgcccgtt cattcttcgg acaccggcat gtacgaaatc gatataacgg ccccgcgctg
     1081 ggacaagtgg tcgatcggca ttgcctcgat gaccgccctg atgcagccgg acggcgccga
     1141 aatggacccg atgccgataa ccgaaaacgc ccgggtcgag atcggcatcg acaacggcat
     1201 cctttcgctt gaccgcaccg gcgcgatttg cgacgaatgc cgccaacgct cgctgggggt
     1261 cgctctacga cgcg
//
LOCUS       DAOPPA010000650         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_116165_length_1267_cov_2.191419, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000650 DAOPPA010000000
VERSION     DAOPPA010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116165_length_1267_cov_2.191419"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKH54_13910"
     CDS             <1..218
                     /locus_tag="PKH54_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01039.1"
                     /translation="LVIRIHHDEDAEVWLNGTHVLSDPGFLNEYLEIPLPATAKRLLK
                     PKDNVLTIHCRQTEGGQFVDAGLGIRK"
     gene            417..>1267
                     /locus_tag="PKH54_13915"
     CDS             417..>1267
                     /locus_tag="PKH54_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD01040.1"
                     /translation="MDIAKEIVPINIQDEMKQSYLLYSMSVIVARALPDVKDGLKPVQ
                     RRILMAMHDLNLGPTAQARKSAKVTGDTSGNYHPHGDQVIYPTIVRMVQPFNSRYPMI
                     LGQGNFGSIDDDPPAAQRYTEVRMSNFAMEMLADIDKNTVDFIPNYDNTRTEPTVLPA
                     KFPALLANGASGIAVGMATNIPPHNLGELCDAITLMVDNPEATIEEIMQRMPGPDFPT
                     AGLILGTKGIYDAYSTGRGSVIMQGQAIIEPMENGKNRIIISELPYQVVKNRLISHIA
                     DLVKNRK"
BASE COUNT          301 a          365 c          368 g          233 t
ORIGIN      
        1 aactggtcat tcgcatacac cacgacgagg acgcggaggt ctggctgaac gggacacacg
       61 tcctgtccga tccgggcttc ttgaacgagt acttggagat accgttgccg gcaacagcca
      121 aacgcctgct gaagccgaag gacaacgttc tgactatcca ctgccgtcag accgaagggg
      181 gccagtttgt tgacgcgggt ctgggcattc ggaagtgatc tcgcgacagg ttggctgaag
      241 ccataaaaac cggaccggat tgacaagacg aaatggcaca ggcgtttggg actgagggcc
      301 tgaacgtgcg ataatgtata caggaaaagc gtcgccgcaa cctgtctgcg tgctcagtcg
      361 gccccgcaag gggccgttgt gtcgcctgcc agggtttgat tgagggaagg ggttacatgg
      421 acatcgcgaa ggaaattgtg ccgatcaaca ttcaggacga gatgaagcag tcgtatctgc
      481 tttattcgat gtcggtcatt gtggcccgcg cgcttccgga cgtgaaggac ggactgaagc
      541 cggtccagcg ccgaatcctt atggcgatgc acgacctgaa cctcggaccg acggcccagg
      601 cgcgaaagtc cgccaaggtc accggcgata cctcgggcaa ctaccacccg cacggtgatc
      661 aggtcattta tccgaccatc gtgcgcatgg tgcaaccctt caactcccgg tacccgatga
      721 tattggggca gggcaacttc ggcagtatcg acgatgaccc gcccgcggcg cagaggtaca
      781 ccgaagtccg catgtcgaac ttcgcgatgg aaatgctggc ggatatcgac aagaacacgg
      841 tcgatttcat tccgaactat gacaacaccc gcactgaacc gacagtcctt cccgccaagt
      901 tcccggcttt gctggcgaac ggggcatcag gcatcgcggt cggcatggcc acgaacatcc
      961 cgccgcacaa tctgggcgaa ctgtgcgacg ccatcacctt gatggttgac aatcccgaag
     1021 caaccatcga ggaaataatg cagaggatgc cgggacccga tttcccgacg gcaggcctta
     1081 tcctcggcac caagggaatc tacgatgcct actccaccgg tcgcggttcc gttatcatgc
     1141 agggccaggc gatcattgag ccgatggaga acggcaagaa ccgcatcatc atcagtgaac
     1201 tgccgtacca ggtcgtcaag aaccgcctga tcagccatat cgccgatctg gtgaagaacc
     1261 gtaaaat
//
LOCUS       DAOPPA010000651         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_116490_length_1264_cov_3.919768, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000651 DAOPPA010000000
VERSION     DAOPPA010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116490_length_1264_cov_3.919768"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1264)
                     /locus_tag="PKH54_13920"
     CDS             complement(<1..>1264)
                     /locus_tag="PKH54_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD01041.1"
                     /translation="FDIEPEDVRRSVFFGLGSDGTVGANKNSIKIIAGDTDNFGQGYF
                     VYDSKKAGAITVSHLRFGPRQIRSSYLIRKANFVACHQYSFLEKYDMLRYAAKGAVFL
                     LNAPYSAEEVWQHLPCEVQKDIIAKQLKFYVIDAYKVARENDMGVRINTIMQTCFFAI
                     SGVLPKDDAIKAIKKAIEKTYGKKGPAIVEKNFAAVDNSVANLHEVKVPEIATSCILR
                     PPAVHPKAPDFVKNVTAPMMSSNGEDLPVSAFPVDGTWPTGTTKWEKRNIALEIPVWD
                     PSLCIQCGKCSLICPHAAIRAKVYGAEHLEKAPATFKCADYKAPDFKGNKWTIQIAPE
                     DCTGCGLCAKICPGKNKAVEGEKALMMRDQLPLRVTEAQNFEFFLGLPNIDRSKLKGG
                     TVKGSQLMEPLFEFSGACAGCGETPYVKL"
BASE COUNT          243 a          375 c          362 g          284 t
ORIGIN      
        1 agcttcacat acggagtttc gccgcagccg gcgcaggctc ccgagaattc gaacagtggt
       61 tccatgagct ggcttccctt gacggtgccg cccttcagct tgctgcgatc gatgttcggc
      121 aggccaagga agaattcgaa attctgcgcc tcggtgacac gcagcggaag ctgatcgcgc
      181 atcatcaatg ccttttcgcc ttccacggcc ttgttcttcc cggggcaaat cttcgcgcac
      241 aggccgcagc cggtgcagtc ctcgggcgca atctggatgg tccacttgtt gcccttgaag
      301 tccggggcct tgtagtcggc gcacttgaaa gtcgccgggg ccttttccag atgctcggca
      361 ccataaacct tggccctgat tgcggcgtgc gggcagatca ggctgcactt tccgcactga
      421 atgcaaaggg acggatccca caccgggatt tccagggcga tgttgcgctt ttcccacttg
      481 gtcgtgccgg tcggccaggt gccatcgacc gggaacgccg aaaccggcag gtcctcgccg
      541 ttgctggaca tcatcggggc agtcacgttc ttcacgaaat ccggggcctt cggatgcacg
      601 gccggcggac gcaggatgca actggtggca atttccggaa ccttcacttc gtgcaggttc
      661 gccaccgaat tatcgacggc agcaaagttc ttttcgacaa tggccggccc cttctttcca
      721 taggtcttct caatcgcctt cttgatggcc ttgatggcat cgtccttcgg caggaccccg
      781 gaaatcgcga agaagcacgt ctgcatgatc gtgttgatgc gaacgcccat gtcgttttcg
      841 cgggcgacct tgtacgcatc gatcacatag aacttcaact gcttggcgat gatgtccttc
      901 tggacctcgc atggcaggtg ctgccagact tcctcggcgg aataaggtgc attcagaagg
      961 aagaccgcac cctttgccgc atagcgcagc atgtcgtact tttccaggaa gctgtactgg
     1021 tggcaggcga caaaattcgc cttcctgatc aggtatgaag accggatctg gcgcggcccg
     1081 aagcgcaggt gcgaaacggt gattgcgccg gccttcttcg agtcatagac gaagtaaccc
     1141 tggccgaaat tgtcggtatc gccggcaata atcttgatcg agttcttgtt ggccccaacg
     1201 gttccgtcgg atcccaggcc gaagaacact gaacgcctga cgtcctcggg ctcgatatcg
     1261 aagt
//
LOCUS       DAOPPA010000652         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_117175_length_1259_cov_3.068937, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000652 DAOPPA010000000
VERSION     DAOPPA010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117175_length_1259_cov_3.068937"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKH54_13925"
     CDS             complement(<1..639)
                     /locus_tag="PKH54_13925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD01042.1"
                     /translation="MTNEGSSPTISLGSLEIFKGVMDNLVEGVYVVDTLRRIVYWNEG
                     AAKITGYTRSQVLGCRCGRDLLAHTDPQGILLCERDCPLEKALRDGIHTEYEAMMQNS
                     AGYQVPVLVEVMPIRSRQGRIVGAAQIFRDHTRFRETADRLSQMKRVAMEDPVTHLGN
                     RRRVETVLQARIREFERYGLSMGVIFADINNFKLINDTFGHDVGDRVLEDVAA"
     gene            993..>1259
                     /locus_tag="PKH54_13930"
     CDS             993..>1259
                     /locus_tag="PKH54_13930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD01043.1"
                     /translation="MSFLLLALLFESGCDASLSGDDIEWVAIPAGGFLMGCPPEDTEC
                     VREDWTIGERPYHWVDVPAFEMARTETTQYQYWKVTGEMPSYYPR"
BASE COUNT          238 a          380 c          355 g          286 t
ORIGIN      
        1 cgccgccacg tcctccagca cgcgatcccc cacatcatgg ccgaacgtat cgttaataag
       61 cttgaagttg ttgatgtcgg caaagatgac gcccatcgag agaccgtaac gctcgaactc
      121 gcggatccgg gcctgaagaa ccgtttccac gcggcgcctg ttcccaaggt gcgtgaccgg
      181 gtcttccatc gcgacccgct tcatctgcga cagtcggtcg gccgtctcgc gaaatcgcgt
      241 gtgatcccgg aagatctggg cggcgccgac gatacgtccc tggcggctgc gaatcggcat
      301 cacttcgacc agaacgggca cctggtaccc ggcgctgttc tgcatcatcg cctcgtattc
      361 ggtgtggatg ccgtcgcgca gcgccttttc cagcgggcaa tccctttcgc acagaagaat
      421 gccctgggga tccgtgtgtg ccagcagatc gcgcccgcac ctgcatccaa gaacctggct
      481 gcgggtgtat ccggtgattt tcgcggcgcc ttcgttccaa tagacgattc gtcgcagcgt
      541 atcgacgaca tagacaccct cgaccaggtt gtccatcaca cccttgaaga tttcaaggga
      601 accaaggctg attgtcggtg aactgccttc gttggtcatt cgccgaatcc ttcgctgatg
      661 atcgattctg tccattacgg gtttcaatcg tcaaggcgac cacccggaaa tccgcgtcgt
      721 taagcgacag aagtcagttg atggatttca ggccggcgtc cctttcagcg gcccccgaag
      781 tcagtccatc ccatgccggc gcccctggct gacatcattt caggcatctg tttcagacaa
      841 tcccgcgatc cggaagtcgt tgacgcccgc gacgtccgtc agaccgttcg tttgaacgtg
      901 cggcgttccc ggtatatcat ggcgaaatgt cgggtaattt cacggttggg ctgcccatgt
      961 ctgaaagacc ttcgcgtatt cattttcgct tcatgtcgtt cttgttactg gcactccttt
     1021 tcgaatccgg ctgcgacgct tccctttccg gcgacgatat cgaatgggtc gcaattccgg
     1081 cgggtggatt cctgatgggc tgtccgccgg aagacaccga atgtgtaagg gaggattgga
     1141 ccatcggtga gcggccgtat cactgggtcg atgttccggc attcgagatg gcgcggacgg
     1201 aaaccacgca gtaccagtac tggaaggtga ctggcgaaat gccgtcgtat tacccccgg
//
LOCUS       DAOPPA010000653         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_118157_length_1252_cov_2.349206, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000653 DAOPPA010000000
VERSION     DAOPPA010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118157_length_1252_cov_2.349206"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKH54_13935"
     CDS             complement(<1..425)
                     /locus_tag="PKH54_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD01044.1"
                     /translation="MIDNGCRLARIFGSVLIFVASVLTMAAVSASAAESSVGWPDLTK
                     PARTVGGGRDDVALLIGIEDYVLVPRVEGARENVESWYRWMLQSRGVPIGNIRLVLDQ
                     DASVEGMRNALQEVVAKANPAGTLWIVFVGHGAPSSDGH"
     gene            complement(477..968)
                     /locus_tag="PKH54_13940"
     CDS             complement(477..968)
                     /locus_tag="PKH54_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD01045.1"
                     /translation="MVAKLIVLALLLAGLILVLAAILRRRQRVLSVGGCQGQAGRYQR
                     QSDVWRLIPTSKNHWTRHEIVLSRGNYRVGRAQGNDIVLDCRFVSREHAVFRVASGWL
                     VLLGSELHSPIFVRCSGASKFRKLGASEEFRFDTGDLLSFNSPDGGMVFQVVPGGREE
                     GRW"
     gene            complement(1049..>1252)
                     /locus_tag="PKH54_13945"
     CDS             complement(1049..>1252)
                     /locus_tag="PKH54_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01046.1"
                     /translation="RAGVFMNGKKIGTGSFKKVLRGLEPESKVRVSVDGGEDCEPSSR
                     SYQAIDLYSAGFVSIRLECLRDN"
BASE COUNT          291 a          410 c          333 g          218 t
ORIGIN      
        1 tcatgtccgt cggaactggg tgcaccgtgc ccgacgaaca caatccacag ggttccggcc
       61 ggattcgcct tggcgacgac ttcctgcaag gcgttgcgca tgccttcgac ggacgcgtcc
      121 tgatccagca ccaggcggat attgccgatc gggactccgc gggactgaag catccagcgg
      181 taccaggact cgacgttttc gcgcgcgccc tcgacgcgtg ggaccaggac gtagtcctcg
      241 attccgataa gcagtgcgac gtcgtcacga ccgccgccca cggtccttgc cggcttggtc
      301 aggtcgggcc acccgaccga tgattccgcg gccgatgcgg aaacggccgc catggtcagg
      361 acggatgcca caaatatcag cactgaaccg aatatgcgag ccagcctgca tccgttgtca
      421 atcatcgcaa acctccggaa aacgaaatgg cgaaattgga tgcgccatta tacacattac
      481 catcgacctt cttcacgacc gccgggaaca acctggaaaa ccatgccgcc gtcgggcgaa
      541 ttgaacgaaa gaaggtcccc cgtatcaaag cggaactcct cgctggcgcc aagcttcctg
      601 aacttcgacg ccccggaaca ccgcacgaaa atcggtgaat gaagttcgct gccaagcaac
      661 acaagccagc cggatgcgac ccggaagacc gcgtgctcgc gggaaacgaa cctgcaatcc
      721 agaacgatgt cgttgccctg ggcccttccg acccggtagt tgccgcgtga caggacgatt
      781 tcatgccggg tccaatgatt ttttgacgtt ggaataaggc gccacacatc ggactggcgt
      841 tgataccgtc ctgcctgccc ctggcaaccg ccgacggaaa gcaccctctg ccgccttcgc
      901 aggatggccg ccagcacaag tatcaatccc gccagaagca gcgcgagaac gatcagtttt
      961 gcaaccatcg gattcataaa gttaaaagcc tgaaactcag ttcattcggg aagccgacga
     1021 attcggcaag accacgaaac cgtcacagct agttgtcgcg aagacactcg agcctgatcg
     1081 agacaaaccc ggcactatac aggtcaatcg cctgatacga acgggaggac ggctcgcagt
     1141 cctccccgcc atcgacactg acccgaacct ttgattccgg ctccagcccc cgcagcacct
     1201 tcttgaagct gccggtccca atcttcttgc cgttcatgaa cacgccggcc cg
//
LOCUS       DAOPPA010000654         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_119096_length_1245_cov_6.108403, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000654 DAOPPA010000000
VERSION     DAOPPA010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119096_length_1245_cov_6.108403"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PKH54_13950"
     CDS             complement(<1..431)
                     /locus_tag="PKH54_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01047.1"
                     /translation="MSRVKFGISNDPADYESLRTLFENIDLVMRHRDTIVATPELRDI
                     RVAGCGTFLLYVGPIRLSLGDLVWLWKDECFWLERDGDDTRYAFCAVGSPLSGGHAMH
                     CWSTLAGDFNYLDRVRGAILSVADVGRCRKERGMVAADNDIP"
     gene            690..>1245
                     /locus_tag="PKH54_13955"
     CDS             690..>1245
                     /locus_tag="PKH54_13955"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOD01048.1"
                     /translation="MTGKIERARGCLLGQLAGDSLGGLVEFMRAAEIRQKYPDGVRDM
                     ADGGTWGSIAGQVTDDSEMALALARTLVEWGSYDAVHVRHAYVDWMKSGPFDIGTTTS
                     AGLRGHPNHDSQANGALMRVSPLGIFCAGRDLQVASDLAAIDAAITHPNPVCVAANRL
                     YVMAIAYAVEHGPSAQDLYGKILEW"
BASE COUNT          256 a          340 c          383 g          266 t
ORIGIN      
        1 gggatgtcgt tgtcggccgc gaccatcccg cgttcctttc tgcaccgtcc cacgtctgca
       61 accgacagga tggcgccgcg gacgcgatcc agataattga aatccccggc cagcgtgctc
      121 cagcagtgca tcgcgtgccc gccagaaagc ggactgccga cagcgcagaa cgcatacctt
      181 gtgtcgtcgc cgtcgcgctc gagccagaaa cattcgtcct tccacagcca gacaaggtcc
      241 cccagagaaa gccgaatcgg gccgacgtaa agcaggaatg ttccacagcc cgccacccgg
      301 atgtcgcgca actccggggt cgccacgatc gtgtcgcgat gtcgcatgac caggtcgatg
      361 ttttcaaaca gtgttcgaag tgattcgtaa tcagccggat cgttggaaat gccgaacttg
      421 acgcgtgaca taaaggcccc ctttttagcc attcaatgct ggcaattagg aataaatcca
      481 catgcgaaac ccgtgtttcg cgaaacggaa ggtatctgaa aaacttcgat ttgtcaaggg
      541 gttatctgca ataatgtatg gtcgcgagtt gggcggcgtt tctgtttggc cgttttgaac
      601 cgtttcgtcc gttgcagtca tttgactgtt gcggtcatgg acaaattcag ccctatcggt
      661 tataaactct cccatgaaaa aggaggccta tgactggaaa aattgagagg gcgcgtggtt
      721 gtctgcttgg tcagcttgca ggtgattcgc tgggtggcct ggtcgagttc atgcgggcgg
      781 cagagattcg ccagaaatac ccggacgggg ttcgggacat ggcggacggc gggacctggg
      841 gctcgatcgc gggtcaggtt acggatgatt cggaaatggc cctggccctg gcaagaacac
      901 tggtggagtg gggaagttac gatgcggtgc atgttcgtca cgcctatgtt gattggatga
      961 agtcgggtcc gttcgatatc gggaccacga ccagtgccgg tctgcgcggc catcccaacc
     1021 atgacagtca ggcgaacggg gccttgatgc gagtgagtcc gctggggatc ttctgtgccg
     1081 gtcgtgacct gcaggtcgcg tcggacctgg cggccatcga cgccgccatc acgcacccga
     1141 atccggtgtg tgtcgccgcg aaccgcctgt acgtcatggc gattgcgtac gccgtcgaac
     1201 atggaccgtc cgcgcaggat ctgtacggca agatccttga atggg
//
LOCUS       DAOPPA010000655         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_119753_length_1240_cov_4.285232, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000655 DAOPPA010000000
VERSION     DAOPPA010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119753_length_1240_cov_4.285232"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKH54_13960"
     CDS             <1..345
                     /locus_tag="PKH54_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01049.1"
                     /translation="DGTAPADDPGSVEGTSLVYDFIRTAPDGDVIEFAFDQAGTSLEA
                     TRKLLGQLLGMMRSGEYPIFPHPTTCPVLSNGDKGHDHCPFKAVCRFHAFPGAEGVPE
                     DEDAESGSGDKE"
     gene            349..>1240
                     /locus_tag="PKH54_13965"
     CDS             349..>1240
                     /locus_tag="PKH54_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD01050.1"
                     /translation="MDNTNHIKVVSAAAGTGKTTRLVREYLGLLSGGIPPDRIVAITF
                     TRKAAAELIEAVSNCLRQSLGEPVPEKFQKDFADCYSDVVPCDPEFIRQALAELPNAP
                     AGTTDSFVLRLLGEFALDARLPTDDGGAPAWLDFPVGQGDAGAVFDAAIRETIDPEDG
                     NVPEAAGRLLEQMGLRELLKVVSQTVRDMPSGIDGSPVELAGGNDVLGKVLSGCRDSL
                     QAVIETNPKIETLPNVNIQKSWPPIAAWRNGGFQGSVPEGLFKWYSAVIKNSTAKERR
                     SLESAFRAIKVDLGLVELSLH"
BASE COUNT          262 a          324 c          399 g          255 t
ORIGIN      
        1 gatggcacgg ccccggcgga tgaccctgga tcggtggaag gtacgtcact tgtttacgat
       61 ttcattcgca ctgcgcccga tggcgacgtc attgaatttg catttgacca ggcaggaaca
      121 agtctggaag caaccagaaa gttgcttggg cagcttctgg ggatgatgcg cagcggtgaa
      181 tacccgatct ttccacatcc gactacatgt ccggtactga gcaatggcga caaaggacac
      241 gaccattgcc ccttcaaggc tgtgtgccgg ttccatgcgt ttccgggcgc cgagggcgtg
      301 cctgaagacg aagatgctga gtcgggttct ggcgacaagg agtaaacgat ggacaacacc
      361 aaccatatca aagtggtatc tgcggcggcc ggtacaggaa agacgacccg gttggtcagg
      421 gaatacctgg gcctgctttc cggcggcatc ccacccgacc gtatcgttgc gatcacattc
      481 acacgcaagg ccgctgcgga attgattgaa gcggtgtcaa actgtcttcg gcagtcgctt
      541 ggcgagccgg ttcccgaaaa gtttcaaaag gactttgccg actgttacag cgacgtggtt
      601 ccgtgtgacc cggaattcat ccggcaggca ttggcggaac tgccaaacgc gccagccggg
      661 acaacggaca gctttgttct gcgtctgctg ggcgaatttg ccctggacgc gcgtctgccg
      721 accgacgatg gcggtgcgcc ggcctggctt gattttccgg tgggccaggg ggatgcgggg
      781 gcggtgttcg atgccgccat tcgcgaaacg attgatccgg aggatgggaa tgtgccggaa
      841 gcggccggcc ggctgctgga acagatgggc cttcgtgaac tgctaaaagt ggtttcgcag
      901 accgtgcgtg atatgccctc cggcatcgac ggttcgcccg tcgaactggc tggcggaaac
      961 gatgtgctgg ggaaggtttt gtccgggtgc cgggattcac ttcaggccgt gattgagacc
     1021 aatccgaaaa ttgaaacgtt gccgaacgtg aatattcaga aatcctggcc tccaatagct
     1081 gcttggagga acggggggtt ccagggatcg gtcccggaag ggctgttcaa gtggtattcg
     1141 gccgtgatca aaaacagcac ggccaaagag aggcgcagtc tggaaagcgc cttccgcgcc
     1201 atcaaagtgg atctgggact tgtcgaactg agccttcacg
//
LOCUS       DAOPPA010000656         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_120378_length_1236_cov_2.283658, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000656 DAOPPA010000000
VERSION     DAOPPA010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120378_length_1236_cov_2.283658"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1236)
                     /locus_tag="PKH54_13970"
     CDS             complement(<1..>1236)
                     /locus_tag="PKH54_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOD01051.1"
                     /translation="RRTFLTESLKALLVGATRRLSGAGGDPVVQLQTNFGGGKTHSML
                     ALFHMFSGASATSLDGIEKVMSEAGVNKMPTARRVVLVGNKISPSTPRVKSDGTVVRT
                     LWGELAWQLGGRPAYDRIRADDENATSPGDVLRELFNEYGPCLILIDEWVAYARQLDD
                     QGGLPGGGFETQFSFAQVLTESAKLAKNCLLVISLPASDTAGSPHVQADDVEVGGIRG
                     REALDRLRNVVGRLESSWRPASAEEGFEIVRRRLFEPLSSEQCRDRDVVARAFADMYR
                     VQHAEFPPECRESDYEKRIRAAYPIHPEIFDRLYNDWSTLVKFQRTRGVLRLMSSVIH
                     SLWEKGDRSPLILPANIAIDDSRVRFELVRYMSDSWDPVIAKDVDGANSLPMRIDAEV
                     PSLGKLAACRRVARTIYLGS"
BASE COUNT          267 a          407 c          332 g          230 t
ORIGIN      
        1 cgacccaaga tatatcgtcc gggccacacg cctgcacgcc gccaattttc ccaggctcgg
       61 aacttcggca tcaatccgca tcggcagtga atttgcgcca tccacatcct tggcgatgac
      121 ggggtcccat gagtcactca tgtacctgac cagttcaaaa cgcacccgcg aatcgtcgat
      181 agcgatgttc gccggcagaa tcaggggact ccggtcaccc ttttcccaca ggctgtggat
      241 gacggacgac atcaggcgca atacgccacg cgtacgctgg aacttcacca gcgtcgacca
      301 gtcgttgtac agccggtcaa aaatttcggg atgaatcgga tacgccgcgc ggatacgctt
      361 ttcgtaatcg gattcgcggc attcgggcgg aaattcggca tgctgaacgc ggtacatatc
      421 tgcaaacgcc cgcgcaacca cgtcgcgatc ccggcactgt tctgatgaca gcggttcaaa
      481 caggcgccgc ctgacgattt cgaagccctc ttccgcactt gcaggacgcc acgacgattc
      541 caggcgcccc acgacattgc gcaggcggtc aagagcctcg cgcccacgaa tgccgccgac
      601 ttccacgtca tcagcctgga catgagggga cccggccgtg tcggacgccg gaagacttat
      661 caccagcagg cagttcttag ccagtttcgc agattccgtc agtacctggg caaagctgaa
      721 ctgcgtttca aatccgccgc caggcagccc tccctgatca tcaagctggc gcgcatacgc
      781 gacccattca tcgatcagaa taagacacgg cccgtattcg ttgaacagtt cgcgcaacac
      841 atcgccggga ctggtcgcgt tttcgtcatc agccctgatg cggtcgtatg ccggccgacc
      901 accaagctgc caggcaagtt cgccccacag cgttctgacc acggtcccat ccgacttcac
      961 gcggggcgtg gacggggaaa tcttgttgcc caccaacacc acgcggcgcg cggtcggcat
     1021 cttattaacg ccggcttccg acatgacctt ttcgataccg tcgagcgacg tcgccgaggc
     1081 acccgaaaac atgtggaaca aagccagcat cgagtgtgtt ttaccaccgc caaaattggt
     1141 ctgaagctgg acgaccgggt cgccgccagc cccggacagt cggcgcgtgg ccccgacaag
     1201 caatgctttc agactttccg tcaggaaggt acgccg
//
LOCUS       DAOPPA010000657         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_127350_length_1188_cov_3.645190, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000657 DAOPPA010000000
VERSION     DAOPPA010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127350_length_1188_cov_3.645190"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKH54_13975"
     CDS             complement(<1..241)
                     /locus_tag="PKH54_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01052.1"
                     /translation="MRRILFPCAILLLAAAGCGENSEPECSQPVSDSPCQIQFGVPNE
                     KTGLTDQQCQPRCNCLGKCFEPPAYTEQDIQDLLAM"
     gene            complement(281..>1188)
                     /gene="trpS"
                     /locus_tag="PKH54_13980"
     CDS             complement(281..>1188)
                     /gene="trpS"
                     /locus_tag="PKH54_13980"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD01053.1"
                     /translation="YLKFQAEGHDCFYFIANYHALTSLKDADALRESTMHLAAGYLAL
                     GIDPERSVFFVQSDVPQVTELCWFLNTVCPVGLMERATSFKDKVARGIAPNMGLFDYP
                     VLQAADISIYDANMVPVGADQKQHIEITRDLVAKFNQAFGVEVLVSPEPYIVQSVAVV
                     PGIDGQKMSKSYDNVIQIFDTPKATAKTCGRIVTDSTPLEDPKDPDKCNVFALLKLFA
                     EPAELQVIADSYRAGGYGYGHAKKRLAELINDHFGDARVRYDELISRPDYLRDILAEG
                     GRKARAAAERTMERVRAASGIMRTH"
BASE COUNT          248 a          349 c          337 g          254 t
ORIGIN      
        1 ccatcgccag cagatcctgg atgtcctgtt ccgtatatgc gggcggttca aaacatttgc
       61 ccagacaatt gcagcgtggc tggcactgct ggtcggtcag gcctgtcttt tcattgggga
      121 cgccgaactg gatctggcac ggcgaatcgc tgaccggctg cgaacattcg ggttcactat
      181 tttcgccgca cccggcggcc gcaagtaaca ggatggcaca tggaaacagg attcttcgca
      241 tgacgttccc cgcatcaata ctggtgttgt gcatcgcgct tcagtgggtg cgcatgatac
      301 ccgacgccgc gcgaacccgt tccatggttc gttcggctgc ggcgcgggcc tttcggccgc
      361 cctctgcaag gatatcccgc aggtaatcgg gacggctgat cagttcgtca tacctgacac
      421 gcgcgtcccc gaagtgatcg ttgataagct cggccaggcg cttctttgcg tggccatatc
      481 cataaccgcc ggcacgatag gaatcggcaa tcacctgaag ctcggctggt tccgcgaaca
      541 gcttcaacag ggcaaaaaca ttgcatttat ccggatcttt cgggtcttca aggggcgtgc
      601 tgtcggtcac aatccggccg cacgtctttg ccgtggcctt cggtgtatcg aaaatctgga
      661 taacgttgtc atatgacttg gacatcttct ggccgtcgat gccgggcacg acagcaacgg
      721 actggacgat gtaaggctcc ggcgacacaa ggacttccac gccgaaagcc tggttaaact
      781 tggccaccag atcccgcgtg atttcgatgt gctgcttctg gtcggcaccg accggtacca
      841 tgttggcgtc atagatgctg atgtcggcgg cctgcaggac cggataatcg aaaaggccca
      901 tgttcggtgc aatgccacgc gcaaccttgt ccttgaacga tgtggcgcgc tccatcaggc
      961 caaccgggca tacggtgttc agaaaccagc acaactcggt cacctgcggc acgtccgact
     1021 gcacgaagaa aacactgcgc tcggggtcga tgccaagcgc caggtatccc gcggcaagat
     1081 gcatggtcga ttcgcgcagc gcgtccgcat ccttcaacga agtcagcgca tgataattag
     1141 cgatgaagta gaagcagtca tgcccctcgg cctgaaactt caaatact
//
LOCUS       DAOPPA010000658         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_128461_length_1181_cov_3.773535, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000658 DAOPPA010000000
VERSION     DAOPPA010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128461_length_1181_cov_3.773535"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1181
                     /gene="glgP"
                     /locus_tag="PKH54_13985"
     CDS             <1..>1181
                     /gene="glgP"
                     /locus_tag="PKH54_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOD01054.1"
                     /translation="AQNLWYSWNWDAVMLFSRMDPVLWEQSGFNPLRMLGMMSTDQLA
                     RLAADDSYVARVRRVRAELEKYLTDRSWADETLGDGVKADPDFLVAYFSTEYGIDKSL
                     PIYSGGLGVLSGDHLKSASDLGVPLVGVGLLYQKGYFRQYLNLDGWQQEVYPDNDWYN
                     MPVSQEIGPDGAQVTVTVDLAGENVVAAVWRAQVGRTPLYLLDTNLPVNSPAAREVTS
                     TLYGGDREMRIRQEIVLGIGGVRALAALKLNPTVYHMNEGHSAFLAIERIRQVMARHG
                     VSRETATEYVISTNVFTTHTPVPAGNEKFDNGLMRRYVERYSNEIGLSWPQFDALGKF
                     SGAQDQFGMTVFALRTSGFANAVSRLHGTVSRHMWKDIWPGLDEAEVPITHVTNGIHT
                     RT"
BASE COUNT          230 a          333 c          359 g          259 t
ORIGIN      
        1 gcccagaatc tctggtattc ctggaactgg gacgccgtga tgctgttttc ccgcatggat
       61 cccgtcctgt gggagcagtc gggtttcaac ccgcttcgca tgctgggtat gatgtcaacc
      121 gaccagcttg cgcggcttgc cgccgatgac agttacgttg cgcgtgtcag acgggttcgg
      181 gccgaactgg aaaagtacct gaccgaccgt tcatgggctg acgaaaccct tggcgacgga
      241 gtcaaggccg atcccgattt tcttgtcgcg tatttttcga ccgaatacgg gatcgataag
      301 tcccttccga tttattccgg cggccttggt gtcctttcgg gagaccactt gaagtcggct
      361 tcggatcttg gcgtgccgct ggtcggcgtc ggtctgcttt accagaaggg gtatttcagg
      421 cagtacctga acctggacgg ctggcagcag gaggtgtacc cggacaacga ctggtacaac
      481 atgccggtca gtcaggagat cggtcccgac ggtgcccagg tgaccgtaac ggtcgatctg
      541 gcaggcgaaa acgtcgttgc cgcggtttgg cgtgcccagg tcggcagaac cccgctttac
      601 ctgcttgaca cgaaccttcc ggtaaacagc ccggccgcac gcgaggtcac cagtacgctt
      661 tacggcggcg accgcgagat gcgcattcga caggaaatcg ttctgggaat cggcggcgtc
      721 cgcgcgcttg cggccctgaa gctgaacccg accgtgtatc acatgaacga gggtcattcg
      781 gctttccttg cgatcgagcg catccgccag gtgatggccc gtcatggcgt atcgcgagag
      841 acggccaccg aatatgtcat ttcgaccaat gtatttacaa cacacactcc ggttccggcc
      901 gggaacgaga agttcgataa cggcctgatg cgtcggtatg tcgaacgtta ttccaacgag
      961 atagggctgt cctggccgca gttcgacgcg cttggcaagt tcagcggggc ccaggatcag
     1021 ttcggcatga ccgtctttgc attgcgcacg tccggttttg caaatgcggt cagtcgtctt
     1081 catggcaccg tatcccggca tatgtggaag gatatctggc cggggctgga tgaggctgaa
     1141 gtcccaatta cacatgtaac aaacgggatc cacacgcgta c
//
LOCUS       DAOPPA010000659         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_130535_length_1168_cov_2.934412, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000659 DAOPPA010000000
VERSION     DAOPPA010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130535_length_1168_cov_2.934412"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1168
                     /locus_tag="PKH54_13990"
     CDS             <1..>1168
                     /locus_tag="PKH54_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOD01055.1"
                     /translation="ITPSSNMGEWADDWSAQGRRNIWGTVPQVTEMQSEGGAAGAVHG
                     ALQAGALTTTFTASQGLLLMIPNMYKIAGELTSFVMHVSARTLATHALSIFGDHSDVM
                     SCRGTGFAMLSSASVQEAHDFALISAAATLKSRVPFLHFFDGFRTSHEVAKIEELTNE
                     DLGHMITDEMVAAHRARCMTPDSPVLRGTAQNPDTFFQAREACNRFYDACPEIVQGEM
                     DRFGELTGRRYSLVEYFGAPDAERVIVLMGSGCDVVHEYVEWAMARGEKIGVLKVRLY
                     RPFPVQAFIDAIPKTVKSIAVLDRTKEPGADAEPLFMDVVTAIHGARAAGKFPLAVEP
                     KLVAGRYGLSSKEFTPAMIKAVYDELGRVSPRARFTVGIHDDVTNLSLDFDKDFD"
BASE COUNT          214 a          354 c          354 g          246 t
ORIGIN      
        1 gattacgcct tcgtccaaca tgggcgagtg ggctgacgac tggtccgcgc agggccgcag
       61 gaacatctgg ggcacggttc cccaggtcac cgagatgcag tccgagggcg gcgctgcagg
      121 cgcggttcac ggcgcgttgc aggccggtgc cctgacgacc actttcacgg catcccaggg
      181 tcttcttctg atgatcccta acatgtacaa gattgcgggc gagcttacat ccttcgtgat
      241 gcatgtgtcc gcaagaaccc ttgccacgca cgcactttcg attttcggcg atcattccga
      301 cgtaatgtcc tgccgtggaa ccggtttcgc gatgctttcg tcggcttcgg ttcaggaagc
      361 ccacgacttt gctcttattt ccgcggcggc gaccctgaag tcaagggtgc ctttcctgca
      421 tttcttcgat ggtttccgga ccagtcacga agtcgccaag attgaagagc ttacgaacga
      481 agacctgggt cacatgatca cggacgagat ggtcgccgcc cacagggcgc ggtgcatgac
      541 cccggatagt ccggttctgc gcggaaccgc ccagaatccc gacacgttct tccaggcccg
      601 cgaggcgtgc aaccgcttct acgatgcctg cccggaaatc gtccagggcg agatggatcg
      661 tttcggcgaa ctgactggcc gtcgttacag cctggtcgag tacttcggcg cccccgatgc
      721 cgaacgggtg atcgtcctga tgggttccgg ttgcgacgtc gtccacgaat acgtcgaatg
      781 ggcaatggcg cgcggcgaga agattggcgt cctgaaggtg cgtctgtacc gcccgttccc
      841 ggttcaggca ttcatcgatg ccattccgaa gaccgtgaag tcgattgcgg ttcttgacag
      901 gaccaaggaa cctggcgccg atgccgagcc gcttttcatg gacgtcgtga ccgcaattca
      961 cggggcgagg gccgccggaa agttcccgct ggccgtcgag ccgaagcttg tcgccggtcg
     1021 ttacggcctg tcttcgaagg aattcacccc ggccatgatc aaggccgtct atgacgaact
     1081 cggcagggtc agccccaggg cgcgtttcac cgtcggcatc cacgacgatg tcacgaacct
     1141 gtcgcttgat tttgacaagg acttcgat
//
LOCUS       DAOPPA010000660         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_131784_length_1160_cov_3.535747, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000660 DAOPPA010000000
VERSION     DAOPPA010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131784_length_1160_cov_3.535747"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKH54_13995"
     CDS             complement(<1..557)
                     /locus_tag="PKH54_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01056.1"
                     /translation="MKRLLAVAIIPLFLAACGGDGNPGTDDGMPDVVGQDIPATDTPI
                     VDVPPEDNGTDSGTELPSDNGGGDGDATTEPYENGATALVAAQADLIAGPNATAVPGD
                     ILIRNKHVRFVIRNQEHSLYSPYGGAIVDADIAREPGDEGHEKFHELFPMTGFARIFN
                     PNQMEIVDDGTYSGTAIVRFKGTDGG"
     gene            846..>1160
                     /locus_tag="PKH54_14000"
     CDS             846..>1160
                     /locus_tag="PKH54_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD01057.1"
                     /translation="MGKERLLVVDDEEDILELLRYNLEREGYAVITAESGEVAVELAL
                     SARPDLILLDLMLPGMNGLDVCRQLSRDDRTAMIPIVILTARAEEADIVAGLELGADD
                     YVS"
BASE COUNT          220 a          327 c          345 g          268 t
ORIGIN      
        1 ccaccgtccg ttcccttgaa gcgcacgatt gccgttccgg aatatgtgcc gtcatcgacg
       61 atttccatct gattcggatt gaagatcctg gcgaatccgg tcatcgggaa cagctcgtgg
      121 aatttttcgt ggccttcgtc gccgggctcc cgcgcgatat cggcatcgac gatggcgccg
      181 ccgtacgggg aataaagact gtgttcctgg ttccttatga cgaacctgac gtgcttgttc
      241 ctgatcagta tgtcgccagg aaccgcggtt gcattcgggc ccgcaatcag gtcggcctgg
      301 gccgccacca gggcggtggc gccgttctcg tatggctcgg tcgtcgcatc gccatcaccg
      361 ccgccgttgt cggaaggcag ttccgtgccg ctgtcggtgc cgttatcttc cggcggcaca
      421 tcgacaatcg gggtgtccgt tgctggaatg tcctgtccaa cgacatccgg catgccgtcg
      481 tcggtaccgg gattcccgtc cccgccgcag gccgccagga aaagcggaat aatcgcaact
      541 gcaagcagtc ttttcatttt tttgaactcc atcatgaagg catttaccac gccgtcggaa
      601 taaaccgtgt gtggtatcgg ggattcaatg acttgggcgg ccatgtgtca agcatatatt
      661 agtaattgat gaggatctcg tcagttattg cttcaggcgt cttgctggga agccgcgata
      721 tccccgaagg aatcggttct taccggaaac taacgtgatt ccaccactga ttgtcgggta
      781 tcatcatcct cgggcaggtg ccgcggtttc tggcgattga tcgcaggacg tcgaaagggg
      841 gcccgatggg aaaggaaaga ctgcttgttg tcgatgacga ggaagacatt ctcgaactgc
      901 tcaggtacaa ccttgaaagg gaagggtacg ccgtaatcac ggcggaatcg ggcgaggtcg
      961 ccgtcgagct tgccttgtcc gcacggcccg acctgatcct tcttgacctg atgcttcccg
     1021 gcatgaacgg acttgacgtg tgccgccaac tgtcacgcga cgacaggaca gcgatgattc
     1081 cgatcgtcat cctgacggcc cgcgccgagg aggcggacat cgttgccggt cttgagcttg
     1141 gcgccgatga ctacgtttcg
//
LOCUS       DAOPPA010000661         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_133806_length_1148_cov_3.002745, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000661 DAOPPA010000000
VERSION     DAOPPA010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133806_length_1148_cov_3.002745"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKH54_14005"
     CDS             <1..798
                     /locus_tag="PKH54_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD01058.1"
                     /translation="TVVLAVALRDDPRLVGFGRVRDDLGDDDSAHANTAAAIETEKLP
                     FAETMRLVLSNKILWVLAIGYFCMNSVRYSFMNWAVTYMADFQGQAIKDSAFKAVALP
                     LIGAIGAVSAGWMSDKLFHKRRAPLCAIMLFSLAVVCIGFILVPKGAVGIATAMLGLA
                     GFLIYGPDMLMSGAATADVHPKAAAAATGFTMAMGNFGAMISGAGIGWLKDVTPTLTA
                     DLRSSLPAGVAAVFSEWTLIFILLAFLAILSAMLMVSIWNAKPKGAK"
     gene            864..>1148
                     /locus_tag="PKH54_14010"
     CDS             864..>1148
                     /locus_tag="PKH54_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01059.1"
                     /translation="MLSRKLLVVAAVTVASLLGVTGCGDDPEPVRMDTFGQQDGEDYD
                     VLGETTEGDVVPDAGRDSGPDNVWEVAPDPGGHDQGSPDVQSDVVEDVMPD"
BASE COUNT          189 a          340 c          378 g          241 t
ORIGIN      
        1 acggtggttc ttgccgttgc gttgcgtgac gacccccggc tggtcgggtt cgggcgcgtt
       61 cgcgacgacc ttggggatga cgacagcgcc cacgcgaaca ccgccgccgc gatcgagacg
      121 gaaaaactgc cgttcgccga aaccatgcgg cttgtcctgt ccaacaagat cctgtgggta
      181 ttggccatcg gctatttctg catgaacagt gtccggtatt ctttcatgaa ctgggcggtc
      241 acctatatgg ccgacttcca gggccaggcg atcaaggaca gcgcgttcaa ggcggtagcc
      301 ctgcctttga tcggcgcaat cggagcggtt tcggccgggt ggatgtcgga caagctgttt
      361 cacaagcgcc gcgcgccgct gtgcgcgatc atgttgtttt cgctggccgt cgtctgcatc
      421 gggttcatcc ttgtgccgaa gggtgcggtc ggtatcgcca cggcgatgct tggcctggcc
      481 ggattcctga tttacggtcc cgacatgctg atgtccggcg ccgccacggc ggatgtgcac
      541 ccgaaggccg ccgccgccgc gaccggcttc acgatggcga tgggcaactt cggcgcgatg
      601 atttcgggcg cggggattgg ctggctgaag gacgtcaccc ccacgctgac ggccgacctg
      661 cggtcgtcgc tgccggccgg cgtggccgca gtcttctccg aatggaccct gattttcatc
      721 ctgctggcct tcctggccat cctgtccgcg atgctgatgg tcagcatctg gaacgccaag
      781 ccgaagggcg cgaagtaatt ccggttcgtc cttttccttc aaagaagtta taatttcccg
      841 aagcatacag gtacgggggt tccatgcttt ccagaaagtt gctcgtggtc gcggcagtta
      901 cggtcgcttc gctgcttggt gtcaccggat gcggcgacga tcccgaaccg gtcaggatgg
      961 acaccttcgg ccagcaggac ggcgaagact acgacgtcct gggcgagacg acggaaggag
     1021 atgtcgtccc cgatgcgggg cgcgattccg gcccggacaa cgtgtgggaa gtggcgccgg
     1081 atcccggtgg ccatgatcag ggatcgccgg acgttcagtc tgacgttgtc gaggatgtaa
     1141 tgcccgat
//
LOCUS       DAOPPA010000662         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_134307_length_1145_cov_3.811009, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000662 DAOPPA010000000
VERSION     DAOPPA010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134307_length_1145_cov_3.811009"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKH54_14015"
     CDS             <1..421
                     /locus_tag="PKH54_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01060.1"
                     /translation="SGSSSRKAVGVQVPPFALNIITAIVIVSGLVSCGGPEARIIDAQ
                     VTHLQGLADILAKHPGDTDKAVAGLQEYVTMNMKSLMDARRDTARYLKGLSEEGRAAF
                     AKKSVERTRELRDRIRTAIATYQERERILHFARQLFL"
     gene            604..>1145
                     /gene="rpsB"
                     /locus_tag="PKH54_14020"
     CDS             604..>1145
                     /gene="rpsB"
                     /locus_tag="PKH54_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOD01061.1"
                     /translation="MPKVSIRSLLEAGVHFGHRTNRWNPKMKQYIFGARNGIHIIDLQ
                     QTARLLNDALNFLGRTAAEGETVMFVGTKAQAQSVVEEECRRCRMPYVTNRWLGGTLT
                     NFVTLRKSLDRIDEIDKLLAEGSVERLQKKEVVRLEKEQARMLKNLRGLRQLKATPGA
                     MVVFDPNREKIAIREANRIG"
BASE COUNT          256 a          342 c          339 g          208 t
ORIGIN      
        1 ttcaggttct agtagtcgca aggctgtggg ggttcaagtc ccccctttcg cacttaacat
       61 cattaccgca atcgttattg tttccgggct ggtttcatgc ggcggcccgg aggcgcgcat
      121 aatcgacgcc caggttacgc acctgcaggg ccttgcggac attcttgcca agcaccccgg
      181 ggacacggac aaggccgtcg cgggactgca ggaatacgtg accatgaaca tgaaatccct
      241 gatggacgcc aggcgcgata ccgcccgata cctgaagggc ctgtcggaag aggggcgggc
      301 ggcgttcgcg aagaagtcgg tcgaacgcac ccgcgaactg cgcgatcgca tcagaaccgc
      361 aatagcaacg taccaggaac gcgaaagaat ccttcacttc gcccggcagc tttttcttta
      421 gttctgcgaa cacaggttgt tgaatttcaa aacggattgt ggtaaacatc cggccccttc
      481 cggttctgta ccggttggcc atcccgtcgc gcattgtgcc gacgggaaat tcacacatcc
      541 tggcagaccc cgtggcggcc gcgtgccgac agagccggga tggaggcata aacggaggtt
      601 ccaatgccca aggtatccat caggtccctt ctcgaagccg gcgtccattt cggtcacagg
      661 acaaatcgct ggaacccgaa gatgaagcag tacatcttcg gcgccaggaa cggcatccac
      721 atcatcgacc ttcagcagac ggcccggctg cttaacgacg cgctgaactt ccttggccgc
      781 acggccgcgg aaggcgagac cgtaatgttc gtcggaacga aggcccaggc tcagtcggtg
      841 gtcgaggaag aatgccgccg ctgccgcatg ccgtacgtca ccaaccgctg gctgggcggc
      901 acgctcacca atttcgtcac cctgcgcaag tcgctggatc gcatcgacga aatcgacaag
      961 ctgctggccg agggatcggt tgaacgcctt cagaagaagg aagtcgtcag gctggaaaag
     1021 gaacaggcca ggatgctgaa gaacctgcgc ggtctgcgcc agttgaaggc aacccccggc
     1081 gcgatggtcg ttttcgatcc gaaccgcgaa aagatcgcga ttcgtgaagc caaccgcatc
     1141 ggcat
//
LOCUS       DAOPPA010000663         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_134486_length_1144_cov_3.780533, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000663 DAOPPA010000000
VERSION     DAOPPA010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134486_length_1144_cov_3.780533"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKH54_14025"
     CDS             complement(<1..571)
                     /locus_tag="PKH54_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD01062.1"
                     /translation="MYTSDQMQIDIGRGSWIRRMFEEGLELKKIHGNDKVFDFTLGSP
                     CVEPPPQFQAALVDVVNDPTPGMHTYMQNPGYPATRVAIARRLTAESGIEYTAENIIM
                     TVGAAGASNVFLRSVLNPGDEVIVIAPFFPEYSFYIHNQRGTQVVSKATPECLPDLDD
                     LRGRLTDRTRVLMLNSPCNPTGRMIPAPIL"
     gene            824..>1144
                     /locus_tag="PKH54_14030"
     CDS             824..>1144
                     /locus_tag="PKH54_14030"
                     /inference="COORDINATES: protein motif:HMM:NF023834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HOD01063.1"
                     /translation="MEYTADLKDIKFLLFEVLKAGDLQKLEKFADFGVDDYEAIIDAA
                     YAFASEVLAPLNDVGDKEGLGFGPDGVTMPTGFIDGWKRYSADGWIGAIVPQEVGGQG
                     LPVLM"
BASE COUNT          223 a          299 c          365 g          257 t
ORIGIN      
        1 tcaggatcgg cgcgggaatc atccgaccgg tcgggttgca cggcgagttc agcatcagga
       61 cgcgggtacg gtcggtcagg cggccgcgaa ggtcgtccag gtccggaagg cattccgggg
      121 tcgcctttga aacaacctgg gttccgcgct ggttgtggat ataaaaactg tattccggga
      181 aaaagggcgc gatcacgata acttcgtcgc ccgggttcag gacgctgcga aggaatacgt
      241 tggatgcccc ggccgcgccg actgtcatga tgatattttc cgcggtgtat tcgataccgg
      301 attcggccgt aagccggcgc gcaatcgcca cccgcgtggc cggataaccc ggattctgca
      361 tgtaggtgtg catgcctggc gtcgggtcgt tgaccacatc gacaagggcc gcctggaact
      421 gggggggcgg ctcgacgcac ggactgccca gagtgaagtc gaaaaccttg tcgtttccgt
      481 ggattttctt cagttcaagt ccttcctcga acatccttcg aatccaggaa cccctgccta
      541 tgtcgatttg catctggtcg gatgtgtaca tctgaacacc tttgaatcaa accgttgcct
      601 gaatatcgta tcggcgtcgc cgggtcaaca gtctccacaa ccggtggccg aacaaatcaa
      661 aataatatta agcaccttcg cagataatcg gttgaccgcg aatgccgttt gtcggaagct
      721 tgtgtttcgt tcctgaattt tccggcgttt cgtgtgttgg tatatggcgc cggggcaagc
      781 cgggctggtc tggtcgcctt gatggcattc acggagggta ccgatggaat acaccgctga
      841 tctgaaggac attaagtttc tgctgtttga agtcttgaag gccggggacc tgcagaagct
      901 ggaaaaattc gcggatttcg gggtcgatga ctacgaggcc atcattgatg cggcgtacgc
      961 gttcgcgtcc gaggtcctgg cgcccctgaa cgacgtcggc gacaaggagg gcctggggtt
     1021 cgggccggat ggcgtcacga tgccgacagg cttcatcgat ggctggaagc ggtattcggc
     1081 ggatggctgg atcggcgcga tcgttccgca ggaagttggc ggtcagggac tccccgtcct
     1141 gatg
//
LOCUS       DAOPPA010000664         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_134997_length_1141_cov_5.704420, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000664 DAOPPA010000000
VERSION     DAOPPA010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134997_length_1141_cov_5.704420"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..317
                     /gene="eutM"
                     /locus_tag="PKH54_14035"
     CDS             21..317
                     /gene="eutM"
                     /locus_tag="PKH54_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HOD01064.1"
                     /translation="MADALGMIETKGFVALVEAADAMVKAAKVELVGYEKIGGGYNTA
                     IVRGDVAAVKAATEAGARQAERVGELVSVHVIPRPHANIDEVLPLGRQGAKAGK"
     gene            471..758
                     /locus_tag="PKH54_14040"
     CDS             471..758
                     /locus_tag="PKH54_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOD01065.1"
                     /translation="MMMGIVVGTVVSTRKNEDLVGLRFLVVNELDQNMKETGKMVVAA
                     DSLDAGVGEVVLYASGSSARQTEATKNRPVDATIMAIVDELAIDGRSRYLK"
     gene            772..>1141
                     /locus_tag="PKH54_14045"
     CDS             772..>1141
                     /locus_tag="PKH54_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOD01066.1"
                     /translation="MAVEEQKISSIVEQVMARLRDEGALPAGHGPVRGPAIVTNVNKP
                     GTFGVHNDIDGCVQAAQAAHEQLMRLSLEVRANAIQAIRDVAVANAPELARKAHEETK
                     MGRVEDKIAKNRLAALKTPGI"
BASE COUNT          235 a          323 c          387 g          196 t
ORIGIN      
        1 ttaagttctg gaggttaacg atggctgatg ctcttggaat gatcgaaacg aagggctttg
       61 ttgcgctggt cgaggcggcc gatgccatgg tcaaggcggc caaggttgaa ctggtcggct
      121 atgaaaagat cggcggcggt tacaacaccg ccatcgtccg tggcgacgtc gccgcggtca
      181 aggcggcgac cgaggccggc gcccgccagg ctgaacgcgt tggcgaactg gtttccgttc
      241 acgttattcc gcgtccgcac gcgaacatcg acgaagtcct gccgcttggc cgccagggcg
      301 cgaaggccgg caagtaggcc cgcggggaat acgtttggcc ggcctgaagc ggttgccgtt
      361 caggcgggcc ggacaagccc tgcctgctga tgttccgtga tcgggcacca ctggtcgccc
      421 gaacgtcaca gggcgccgtg tcggacccag agtccgaagg gaggactgcc atgatgatgg
      481 gaatcgtggt gggtaccgtc gtatccacca ggaaaaacga ggatctggtc gggctccgct
      541 ttctggtcgt caacgaactg gatcagaaca tgaaggagac cggcaaaatg gtcgtggcag
      601 ccgattctct ggacgccggc gttggtgaag tcgtccttta tgccagcggc tcgtcggcca
      661 ggcagactga agcgacgaag aatcgtccgg ttgacgcgac gatcatggcg atcgtcgatg
      721 aactggcgat cgacggcagg tctcgctacc tgaagtagcc agggggccac aatggctgtt
      781 gaagagcaga aaatatcctc aatcgtggaa caggtgatgg cccgcctgcg ggacgaggga
      841 gccctgccgg ctggacacgg cccggttcgg ggcccggcca tcgtgaccaa cgtcaacaag
      901 ccaggcacct tcggtgtcca caatgacatc gacggctgcg tgcaggcggc ccaggcggcg
      961 cacgaacagt tgatgcgtct ttcactcgag gttcgcgcca atgccatcca ggccatccgg
     1021 gatgttgcgg tggcgaacgc gcccgagctt gcgcgcaagg cccacgaaga aacaaagatg
     1081 ggccgcgtcg aggacaagat tgccaagaac aggcttgcag ccttgaagac cccgggaatc
     1141 g
//
LOCUS       DAOPPA010000665         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_138691_length_1120_cov_9.932394, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000665 DAOPPA010000000
VERSION     DAOPPA010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138691_length_1120_cov_9.932394"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1120)
                     /locus_tag="PKH54_14050"
     CDS             complement(<1..>1120)
                     /locus_tag="PKH54_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOD01067.1"
                     /translation="KGLANLGIKILAGSMQYTVQRGRLEKKFATPPDGRVTPVGQLES
                     WERTDSGATFRFAGAELEVVFVLPGIPRLTWTPGVLPGNCVVTRTSFNPAQVAVEETD
                     SGISLENNGLRLTIGRDGSVTATDSDGNTIRRDEPPVMTGAGWTSRATMNDDESFFGM
                     GMKASPFSVRGCEFSLWNMEPRGAYAAGDDPLYISSPAYLCVSPRGSYLAFFDNTFKS
                     TFSFKERLESRFEGGALRYYLFAGTPEDNVARFYGLIGHAAMPPRWALGYHQCRWSYM
                     NEAEVREIADGFASRDLPLSAIHLDIHYMDGYRVFTVDPARFPDLKKLSEDMLASGIR
                     LVTILDPGVKADPEYFLFKEGCDGNLFMKLPDGEQVRGV"
BASE COUNT          210 a          342 c          349 g          219 t
ORIGIN      
        1 ccactccgcg gacctgttcg ccatccggaa gcttcatgaa cagatttccg tcgcagcctt
       61 ccttgaacag gaagtattcc gggtcggcct ttactcccgg atcgaggatg gtgaccagcc
      121 tgatcccgga ggccagcatg tcttcgctga gcttcttcag atccgggaaa cgggcggggt
      181 ccaccgtgaa tacgcggtat ccgtccatgt aatggatgtc gaggtggatg gccgaaaggg
      241 gcaggtcgcg gctggcgaaa ccatccgcga tctcacggac ctcggcctcg ttcatgtagc
      301 tccagcgaca ctggtggtat ccgagggccc agcggggcgg catggccgcg tggccgataa
      361 ggccgtagaa cctggcgacg ttgtcttccg gggtgccggc gaacagatag tacctgagcg
      421 cgccaccctc gaaccgggac tcgagccgtt ccttgaaaga aaaggtgctc ttgaaggtgt
      481 tgtcgaagaa ggccaggtac gaacctcgcg gggacacgca gaggtacgcc ggcgatgaga
      541 tgtaaagcgg gtcgtcaccg gccgcgtatg cgccacgcgg ttccatgttc cacagggaga
      601 actcgcatcc gcgcaccgag aagggcgatg ccttcatgcc cattccgaag aacgactcgt
      661 cgtcattcat cgttgcccgg gatgtccagc cggccccggt catcacggga ggttcgtcac
      721 gtcgaatcgt gttgccgtcc gagtcggttg ccgtcaccga tccgtccctg ccaatcgtca
      781 gtctcagtcc gttgttttcc agggagattc cggaatcggt ctcttcaacg gcgacctggg
      841 ccgggttgaa tgaggttctg gtgacgacgc agtttcccgg caggaccccg ggggtccagg
      901 tcaacctggg aatccctggc agtacgaaaa ccacctcaag ctcggcgccg gcaaagcgaa
      961 acgtcgcccc ggagtccgtt cgttcccagg actcaagctg tccgacaggc gtgaccctgc
     1021 cgtccggcgg cgtggcgaac ttcttttcga gcctgccgcg ctgaaccgtg tactgcatgg
     1081 aacctgccag aatcttgatg cccagattgg cgagtccctt
//
LOCUS       DAOPPA010000666         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_139438_length_1116_cov_4.223374, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000666 DAOPPA010000000
VERSION     DAOPPA010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139438_length_1116_cov_4.223374"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..895)
                     /gene="rplB"
                     /locus_tag="PKH54_14055"
     CDS             complement(68..895)
                     /gene="rplB"
                     /locus_tag="PKH54_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD01068.1"
                     /translation="MGLKNYKPTSAGRRGMQGDDFADITASKPEKKLTTGARESGGRN
                     NYGRITSRHRGGGHKQLFRVIDFKRDKENVPAKVATIEYDPNRSARIALLHYVDGEKR
                     YILAPSGIAVGDMIVSSLHAEIRPGNCLPLKAIPQGSQVHNVEIKPGHGGQLVRSAGS
                     SAQVMAFEGGYVLLRMPSGELRKVLAECRATLGELSNHEHENMSIGKAGRNRWKGVRP
                     QSRGVVMNPVDHPMGGGEGKTSGGRHPCTPWGKPTKGYKTRKNVSTDRLIVKRCNAK"
     gene            complement(933..>1116)
                     /locus_tag="PKH54_14060"
     CDS             complement(933..>1116)
                     /locus_tag="PKH54_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD01069.1"
                     /translation="RQEVKEAVEAVFQVKVEAVRTVIVRGKAKRVGKHVGRRPDWKKA
                     VVTLREGDSIDLFEGM"
BASE COUNT          193 a          366 c          311 g          246 t
ORIGIN      
        1 cttctcgacc agatgcttgt caatgaacgg tcccttcttt actgacctgg gcatctcaaa
       61 ctccgttcta cttcgcgttg caccgcttca cgataagacg atcggtcgaa acgttcttcc
      121 gagtcttgta accctttgtg ggcttacccc acggggtgca ggggtgacgt ccaccggacg
      181 tcttgccttc accaccgccc atcggatggt caaccgggtt catgacgacg ccgcggctct
      241 gcggacgaac gcccttccac cgattacgac cggccttgcc gatggacatg ttctcgtgct
      301 cgtggttcga aagctcgccg agggtcgcgc ggcattccgc gagaaccttg cgaagctcgc
      361 cggacggcat gcgaagaagg acgtatccgc cttcaaatgc catgacctgg gccgaactgc
      421 cggccgagcg gacaagctgt ccaccgtgac ccggcttgat ctcgacgttg tgaacctgcg
      481 aaccctgcgg aatcgccttc aggggcaggc agttacccgg cctgatttcg gcatgcagcg
      541 acgaaacgat catgtcgccc acggcgatgc cggacggggc caggatgtaa cgcttttcgc
      601 catcgacgta gtgcagcagg gcgattcggg ccgaccggtt cgggtcgtac tcaatcgtcg
      661 cgaccttggc cggcacgttt tccttgtccc gcttgaaatc gataacgcgg aacaactgct
      721 tgtggccacc gccacgatgc ctggacgtga tgcggccgta attgttcctg ccgccgcttt
      781 ccctggcgcc ggtcgtcagc ttcttttcgg gctttgatgc ggtgatgtcg gcgaaatcgt
      841 cgccctgcat cccgcgtcgt cccgctgaag tcggcttata gttcttaagt cccatctttc
      901 agctcccagg cggccatcag gccccgtgaa gattacatac cctcaaacag gtcgatgctg
      961 tcgccctcgc gaagggtgac aaccgccttc ttccagtcag gcctgcggcc gacatgcttg
     1021 ccgacgcgct ttgccttgcc gcggacgata accgtcctga ccgcttcgac cttcacctgg
     1081 aaaacggcct cgaccgcttc cttaacttcc tggcgg
//
LOCUS       DAOPPA010000667         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_140245_length_1112_cov_2.363292, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000667 DAOPPA010000000
VERSION     DAOPPA010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140245_length_1112_cov_2.363292"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>506)
                     /locus_tag="PKH54_14065"
     CDS             complement(<1..>506)
                     /locus_tag="PKH54_14065"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOD01070.1"
                     /translation="PTETSSSPGRYSVDLTPYVREIQDRLHPDDPAQVIVYEAAAQAG
                     SKSTIAENWVMAVAGGFYPARMLYALDTDSNAQDWSKDNLDTMIDHSRLLRERVRNPV
                     SRGKNETILGKWFPGGRLRIIGAHSASAMCRIAAKYVVMDECDRWKENPGYEGNGVSL
                     ILARQTTF"
     assembly_gap    507..516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(628..930)
                     /locus_tag="PKH54_14070"
     CDS             complement(628..930)
                     /locus_tag="PKH54_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01071.1"
                     /translation="MDMAVGDSARLLKARAISATADAKLKQLRIQERLRELVPRAAVT
                     QAFADTLAAARSQLEAMPTRLSHRLVGLEATEIRNLLQTEIDAVLRELADGLDPDR"
     gene            complement(894..>1112)
                     /locus_tag="PKH54_14075"
     CDS             complement(894..>1112)
                     /locus_tag="PKH54_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01072.1"
                     /translation="MFRQPVPARIPVAIRRTAAGRIEPSVARRHPISLHLNRSALVPS
                     LLIDRQPSTPTFQRTAAWTWPSVIRPGY"
BASE COUNT          191 a          340 c          349 g          222 t
ORIGIN      
        1 gaatgtcgtc tgccgcgcca gtatcagcga aacgccatta ccctcgtaac cggggttttc
       61 tttccagcgg tcgcactcgt ccatgacgac gtacttggcc gcgatacggc acattgccga
      121 tgccgagtgg gccccgatga tccgaaggcg accgccggga aaccacttac caagaatcgt
      181 ttcattcttg ccacgcgaca ccgggttgcg gacgcgttcc cgcagcaacc ggctgtggtc
      241 gatcatcgtg tccaggttgt ccttcgacca gtcctgagcg ttgctgtcgg tatccagcgc
      301 atacagcatc ctggccgggt agaatccgcc ggcgactgcc atgacccagt tttccgcgat
      361 ggtcgatttc gaccctgcct gcgcggcggc ctcgtaaacg atgacctgcg cggggtcgtc
      421 cggatgcagg cggtcctgga tttcacggac gtaaggggtc aggtcaacgc tgtaacggcc
      481 aggtgatgac gacgtttcag tcggtannnn nnnnnnatca cgcggtggtg ctgggcgata
      541 tcggtgatgg acagccgagg tctggggcgc agcgcatcgg cgcctgcctt gcgaaacagt
      601 cgagcggctt cttcccaggc cgccatgtca tctgtccggg tcgagtccat ctgccaattc
      661 cctcaatacc gcgtcgattt ccgtttgaag aaggttccga atctcggtcg cttccaggcc
      721 caccaggcga tgcgacaggc gggtcggcat cgcttccaat tgcgacctgg ctgccgccaa
      781 cgtatccgcg aacgcttgag tgaccgccgc acggggcacc aattcccgca gacgttcctg
      841 gatacgaagt tgtttcagtt tggcgtcggc cgtcgcgctg atcgcacggg ccttcagtaa
      901 ccgggccgaa tcaccgacgg ccatgtccat gccgctgtcc gctgaaaagt gggtgtgctg
      961 ggctggcggt cgataagcag gctcgggacg agggcgctgc ggttcaggtg caggctgatg
     1021 ggatgacgac gcgccaccga aggctcgatc ctgcctgccg ccgtgcgtcg gatcgcgacc
     1081 ggaattcgcg cgggcacggg ttgccgaaac at
//
LOCUS       DAOPPA010000668         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_140411_length_1111_cov_3.065341, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000668 DAOPPA010000000
VERSION     DAOPPA010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140411_length_1111_cov_3.065341"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKH54_14080"
     CDS             complement(<1..477)
                     /locus_tag="PKH54_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOD01073.1"
                     /translation="MFQSGQAENKRILLVNVERTATRSPNLGLGYLASVIRRQGWDVR
                     LFDAAQKGAGTTALLSALREGPWDIIGFQAYSSGYPITRAAARIARKLNPDTPILLGG
                     RHASPLAEEALGEEEAFTWAVVSEGELSLQELAAHAGAGSIPDEILATIPNLVRRTP"
     gene            complement(550..939)
                     /locus_tag="PKH54_14085"
     CDS             complement(550..939)
                     /locus_tag="PKH54_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD01074.1"
                     /translation="MNICVPVTEDIGIDSPVCGHFGSAPGFIVVDTESGACKSIRNMN
                     LHHQHGMCQPLLSLAGHQIDGIVVGGIGAGALNKLLAGGIRVYLAQQPTVRDAIDAFK
                     AGTLREVDPSMACGGHGHGHGNGHSCH"
BASE COUNT          207 a          356 c          333 g          215 t
ORIGIN      
        1 cggcgtccgc cggacaagat tcgggatcgt cgccaggatt tcatcgggaa tgcttcccgc
       61 gccggcgtgc gcggccagtt cctgaagcga cagttcgcct tccgagacga ctgcccaggt
      121 gaatgcttcc tcttcgccaa gggcttcttc cgccagcgga cttgcgtgtc tgccgccaag
      181 aagaatcgga gtgtcgggat taagcttcct ggcgattctg gcggcggccc tggtgatcgg
      241 gtaacctgac gaataagcct ggaagccgat gatgtcccac ggtccctcgc gaagcgccga
      301 aagcagggcc gtggttcccg cccctttctg cgccgcgtca aaaaggcgaa catcccagcc
      361 ctggcgccgg atcaccgaag cgagatatcc cagtcccaga ttgggtgaac gggtcgcggt
      421 tctttcaacg ttgaccagca ggattcgctt gttttcggcc tgaccggact ggaacaaatc
      481 cgccccccag aaacgcccaa agcggcagcc gcatcattat atgcccatac ggatgccatt
      541 gaaagacggt cagtggcagg agtggccgtt gccgtggccg tgtccgtggc cgccacacgc
      601 catcgacgga tcgacctccc tcagggtacc ggctttgaac gcatcgattg catcgcgcac
      661 ggtgggctgc tgggccagat acacccggat cccgccggca agcagcttgt tcaaggcccc
      721 ggcgccgatt ccgccgacca cgatgccgtc aatctggtgg ccagccagcg acaacagcgg
      781 ctggcacatc ccgtgctggt ggtgcaggtt catgttccgg atgcttttgc acgcaccgga
      841 ttccgtatca acgacgatga atcctggggc ggagccgaaa tgtccgcaaa cggggctgtc
      901 aatgccgatg tcttcagtaa ccgggacgca gatattcatt tgattcctcc atcgcacctg
      961 cgagtctgga cggcgcccgc aggcccgtca gccgcattcc tgcaccgggg acaacgcctg
     1021 ccgttgcctt cgatgcgcat gcacttcggg caatcgttgc ccgagccgtt gtcggtggtg
     1081 accgttccac cctggatacg gagttcaaga c
//
LOCUS       DAOPPA010000669         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_144807_length_1088_cov_2.778316, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000669 DAOPPA010000000
VERSION     DAOPPA010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144807_length_1088_cov_2.778316"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..377
                     /locus_tag="PKH54_14090"
     CDS             129..377
                     /locus_tag="PKH54_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01075.1"
                     /translation="MYEVASPQTLERFTLAPRGTIYGWHQVPEQSLLQRLPQEVPEIP
                     NLYLAGAWTFPGCGQSAVLQSGVIAAGKILKADAESAE"
     gene            complement(442..654)
                     /locus_tag="PKH54_14095"
     CDS             complement(442..654)
                     /locus_tag="PKH54_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01076.1"
                     /translation="MTSGVASVEVPYGTVDGCFLYSVDAWEAMGQEDPMGPMHVDVYV
                     KPDFGIVRISMVPGYDAMELVSVTFP"
     gene            742..>1088
                     /locus_tag="PKH54_14100"
     CDS             742..>1088
                     /locus_tag="PKH54_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01077.1"
                     /translation="MKGFLIVSILAVLAFAACGSNEGGSDQGGNHDADYDIPGYDATG
                     DTVAGDSGRDAAGNDGTGDDIQGQDVPAETSLPDTDEDQGQSDVPADVEEDTGTVELC
                     PYPTKIEIEDFNR"
BASE COUNT          226 a          332 c          326 g          204 t
ORIGIN      
        1 tttaccgccg accgccaacc atcaccgggc gtactccctt tcctatgagg ggatgactca
       61 gcgcggtcgc aaggacgttc atcgaacgcg cggaggctgt cctgcccgag ctttcgcgcc
      121 acgtcgtcgt gtatgaagtc gcgtcgccac agaccctcga gcggttcacg ctggccccgc
      181 gcggcaccat ttacggctgg caccaggttc ccgaacagtc cctgttgcag cgccttccgc
      241 aggaagtccc cgaaattccc aacctttacc tggccggcgc ctggacgttc ccgggatgcg
      301 gccagtccgc agtcctgcag agtggtgtca tcgccgcggg aaagatcctc aaggccgacg
      361 ccgaatctgc cgaatagacg caggttcggg gccgttgttc aacagattca aatcattgat
      421 ttcgagggat tggccaaggg cctacgggaa ggtgaccgac accagttcca tcgcgtcgta
      481 tcccggaacc atcgagatgc ggacgatgcc gaagtcgggc ttgacataga cgtcaacgtg
      541 catcgggccc atgggatctt cctggcccat tgcttcccac gcgtccacgg aatacaggaa
      601 gcatccatcg acggttccgt acggaacttc gaccgacgca acacctgatg tcatcgactg
      661 ccgcaacttc cagtttgcgg gcgggctttc atgccgaatg cccgggtgta taatccgcgc
      721 atctcttcct gggaacataa aatgaaagga ttcttgattg tgtcgattct ggctgtgctt
      781 gcattcgcgg cgtgcgggtc gaacgaaggc ggcagcgatc agggcgggaa ccatgacgcg
      841 gattatgaca tcccgggtta tgacgccact ggggacactg tcgcgggcga ttccggacgc
      901 gacgccgcgg gtaacgacgg gactggcgac gacatccagg gacaggatgt accggccgaa
      961 accagcctgc ctgacacgga cgaagaccag gggcagtccg atgttccggc cgacgtcgaa
     1021 gaggacaccg gcacggtcga actttgtccg tacccgacaa agattgaaat cgaggatttc
     1081 aaccgcag
//
LOCUS       DAOPPA010000670         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_145207_length_1086_cov_2.971872, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000670 DAOPPA010000000
VERSION     DAOPPA010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145207_length_1086_cov_2.971872"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PKH54_14105"
     CDS             complement(<1..1069)
                     /locus_tag="PKH54_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOD01078.1"
                     /translation="MVSNRETRVRLVFEGINWSADIWVNGRMISSAETTKGTFREFRL
                     DITDVCEPGANCAIAAKVNVPDIFADLSIYWVDWNPEPPDYNMGLWMPARLDAVGPVT
                     IFDPAILTDIREDGSACLTLVADLENGSDRAVDVELVAEFDGRRITVPVSVCPRQVIE
                     VIRTPAEIADLKLESPILWWPYHYGTPHLYDAKVSAIVDGTVSDSTSFAFGVREVTAV
                     HTNPGSIQFFINGRPILIRGAGWAPDMFLRHDPARDLAELSYVRDLGLNTVRLEGKLE
                     DHAFYDLADRMGILLMPGWCCCDTWESWDTWDQEDYLIADESILNQLRRLRRHASVFT
                     WLNGSDFHPPPDVEQMYLETAA"
BASE COUNT          220 a          320 c          324 g          222 t
ORIGIN      
        1 tggctgccgt ttccaggtac atctgttcaa cgtccggcgg cggatggaaa tccgatccgt
       61 taagccaggt aaagacgctt gcgtgcctgc ggagcctgcg caactggttc aggatgcttt
      121 cgtcggcaat caaatagtct tcctgatccc acgtatccca cgattcccat gtgtcgcagc
      181 agcaccagcc cggcatcagc aggataccca tgcggtcggc caggtcgtaa aaggcatggt
      241 cttccagctt tccttcaagg cgaacggtgt tcaggccaag atccctgacg tacgagagtt
      301 cggccaggtc gcgggccgga tcgtgccgca ggaacatgtc gggcgcccat ccggcgccgc
      361 gaataaggat tggcctgccg ttgatgaaga actggatgct tccgggattc gtgtggacgg
      421 cggtgacctc gcgcacgccg aaggcgaagc tggtcgagtc ggacacggtt ccgtcaacaa
      481 tcgccgaaac cttcgcatcg taaagatgcg gtgtgccgta atgatagggc caccaaagga
      541 ttggcgattc cagcttcaga tccgcgattt ctgccggggt tcggatgact tcgataacct
      601 gccgcgggca gacgctgacc ggcaccgtga tcctgcgtcc gtcgaattcg gcgacaagtt
      661 caacatcaac agcgcgatcc gaaccgtttt cgagatcggc gacaagggtc aggcatgccg
      721 atccatcttc cctgatgtcg gtcaggattg ccgggtcgaa gatcgtcacg ggaccgacgg
      781 catcgagtcg tgccggcatc cacaggccca tgttgtagtc gggcggctcc ggattccagt
      841 caacccagta gatcgaaagg tccgcgaata tgtccggcac gttgaccttt gctgcaatcg
      901 cacagttcgc gccaggttcg cagacgtcgg ttatatccag gcgaaattcc cggaaggtgc
      961 ccttggtggt ctcggcggaa gagatcatcc tgccgttgac ccagatatcg gccgaccagt
     1021 tgatcccctc gaataccagc ctgacccgcg tttcacggtt cgagaccatg ggcaccgtga
     1081 cttcgg
//
LOCUS       DAOPPA010000671         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_147537_length_1074_cov_14.047105, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000671 DAOPPA010000000
VERSION     DAOPPA010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147537_length_1074_cov_14.047105"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKH54_14110"
     CDS             <1..508
                     /locus_tag="PKH54_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD01079.1"
                     /translation="MFHALMEQWGFRFDLGSPGDCFDPGYLRAFAARFETRSGVDHAM
                     WLRKALELFPGHPLYAELRAAANRGELFHEVQVSALLPPDATLPGGLDVAGRIDLLFR
                     DAAGRWCVIDYKETRKVTTAEELQGLKRDYGPQLSLYRAVLESWKPGQVGRLGLWLVL
                     AGDVMWME"
     gene            552..>1074
                     /locus_tag="PKH54_14115"
     CDS             552..>1074
                     /locus_tag="PKH54_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOD01080.1"
                     /translation="MGRSRERTITVEEILASCRRWGVPHFQRGGVWDMGNRAALLESL
                     FFDTPCGSVILWDKPDKDFGIPLYENGVAAAPAGSATAIPVSRNNAPFDFLIIDGQQR
                     TRSLMAAFEGVEDFAAADTEESQEQDSGTEASSTSPQPLKKYWSINLESLKEFGFKDM
                     LDPEGKAVAAPLFV"
BASE COUNT          198 a          316 c          346 g          214 t
ORIGIN      
        1 gatgttccac gccctgatgg agcagtgggg gttcaggttc gaccttggtt cgcccggcga
       61 ctgtttcgac cccggatacc ttcgcgcgtt cgcggcccgg ttcgaaacca ggtccggcgt
      121 cgaccacgcc atgtggcttc gcaaggccct ggaactgttc cccggccatc cgctgtacgc
      181 tgaactgcgc gctgcggcca accgtggcga gctgttccac gaggtgcagg tttcggcgct
      241 gttgccgcct gacgcgacct tgcccggcgg cctggacgtt gcgggccgaa tcgacctgct
      301 gttcagggac gccgccggcc gctggtgcgt catcgattac aaggaaaccc ggaaggtcac
      361 gaccgcggaa gaactgcagg gcctgaaacg cgactacggc ccccagcttt ccctgtaccg
      421 cgccgtcctg gaatcttgga aacccggcca agtcggccgc ctgggcctct ggctggtcct
      481 cgccggcgat gtgatgtgga tggagtaagt gttgtgtgtg cctcgtttga aggcttcagg
      541 gggttttgat aatgggacgc tcaagggaac ggacgatcac ggttgaagaa attcttgcca
      601 gttgccggcg atggggtgtg ccacatttcc agcggggcgg cgtctgggac atgggaaacc
      661 gggccgcgct tcttgaatcg ctgtttttcg atacgccgtg cggcagcgta atcctgtggg
      721 ataagccgga taaggatttt gggatcccgt tgtacgaaaa cggcgtcgct gccgcgcctg
      781 ccgggtcggc cacggccatt ccagtatccc ggaacaatgc gccctttgac ttcctgatca
      841 tcgacggcca gcagcgaacc agaagcctga tggccgcctt cgagggggtc gaggatttcg
      901 ccgccgccga tacagaagaa agtcaggaac aagactccgg aaccgaggcg tcttcaactt
      961 caccgcaacc cctgaaaaag tactggagca tcaacctgga aagcctgaag gaattcggct
     1021 tcaaggacat gcttgatccg gaagggaaag ccgtggcggc gccgctgttc gttt
//
LOCUS       DAOPPA010000672         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_147565_length_1074_cov_3.933268, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000672 DAOPPA010000000
VERSION     DAOPPA010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147565_length_1074_cov_3.933268"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKH54_14120"
     CDS             <1..715
                     /locus_tag="PKH54_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD01081.1"
                     /translation="LKEFEQIVSDNRGQIAMFISTPYHHPVFTDNVLPAEGWWTGVEK
                     ICRENGIVLAIDDIRAGFRCALGGSADYFGFKPDLACYCKAIANGQTISALVGTNALR
                     NAVSDVFFTGSYWSGSVAMAGAIATIKELQRLDGANMMIDKGRRLTDGMVKAAADCRL
                     NLKVSGMPSMFYIRITDDDSLMMTQEFTAECTRRGAFLVSHHNHFINCSLTDTDIAET
                     IEIAHEAFRVVAKRHGRLD"
     gene            735..>1074
                     /locus_tag="PKH54_14125"
     CDS             735..>1074
                     /locus_tag="PKH54_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01082.1"
                     /translation="MMHSDRNGEMMGNWFLKCCCAVAALAVVLAGVSAMAFEMDGQDS
                     VAGRYFLASSFDGVDLPALVRQANGRILVEMPPDRVVAKFASQSDAAALLNDFRLRTV
                     AAFSGPGESWL"
BASE COUNT          219 a          281 c          331 g          243 t
ORIGIN      
        1 cctgaaggag ttcgaacaga tagtcagcga taaccgtggc cagatcgcca tgttcatctc
       61 gacgccgtat caccatcccg tgtttaccga taacgtcctt ccggccgaag gctggtggac
      121 gggggtcgaa aagatctgcc gtgaaaacgg catcgttctt gcgattgatg acattcgtgc
      181 cggattccgc tgcgcgcttg gcggatcggc cgactatttc ggcttcaagc ccgacctggc
      241 gtgttactgc aaggccattg ccaacggcca gacgatttcg gcccttgtgg gtaccaacgc
      301 gcttcgcaac gcggtatccg acgtgttctt caccggaagt tactggtccg gcagcgtcgc
      361 gatggccggg gcgatagcga cgattaagga acttcaaagg cttgacggcg ccaacatgat
      421 gatcgataag ggacgccgcc ttaccgacgg aatggtcaag gcggcggctg actgtcgcct
      481 gaatttgaag gtttccggga tgccatcgat gttctatatc cggattaccg atgacgacag
      541 cctgatgatg acccaggaat ttaccgccga gtgcacccgg cgtggggctt tccttgtcag
      601 tcaccataat cacttcatca attgcagtct taccgacacg gacatcgccg aaacaatcga
      661 aatcgcccac gaagcgttca gggtcgtcgc gaaaaggcac ggcaggctgg actgaacggt
      721 gtgcgggaat atccatgatg cattcagata gaaacgggga aatgatggga aactggttcc
      781 tgaagtgttg ctgtgctgtt gccgcgcttg ccgtggttct tgccggtgtg tcggcaatgg
      841 cgtttgaaat ggatgggcag gattccgttg cgggacgata tttccttgct tccagctttg
      901 acggcgtgga tctgccggct ctcgtgcggc aggcgaacgg aaggatcctg gtcgaaatgc
      961 cccccgacag ggtggtggca aagtttgcga gccagtctga tgcggccgca ttgttgaacg
     1021 atttccgtct tcgaacggtg gctgcgtttt ccggtccggg cgaaagctgg ctgg
//
LOCUS       DAOPPA010000673         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_152888_length_1049_cov_2.388330, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000673 DAOPPA010000000
VERSION     DAOPPA010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152888_length_1049_cov_2.388330"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="PKH54_14130"
     CDS             <1..>1049
                     /locus_tag="PKH54_14130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOD01083.1"
                     /translation="VSPAASNSWAVAGSHTESGKPILANDPHLLHGAPATFYIVHLKA
                     PGIDAIGATLPGTPFLTLGRNQKVGWASTNTFADVQDIYLEKLDPNDPGKYMTPQGPV
                     AFEIEKHVLRERTGRDEYKEHKFDLRYTRHGAVLNDAMIDGLPGQPIALKTASIWPTD
                     ESLSMKKYLTATSIDDIFNALQSWGLPIQNWVAVDDAGHIGYFPMGVVPIRRTFDGTV
                     PVPGWTDEFEWDGFIPYDQLPKMIDPPSGMIVTANNQVMPAQDYPYPYSIDTMQGYRA
                     GRIRELLKAGGKWTADGIRTMQMDAHVPQADRLLPSLLKALDGADLDDFEKSALARLK
                     GWDRVAAIDSVGASV"
BASE COUNT          221 a          336 c          314 g          178 t
ORIGIN      
        1 acgtcagccc ggccgcatcc aacagttggg cggttgccgg aagccacacg gaaagcggca
       61 agccgatcct ggccaacgac ccgcatctgc ttcacggggc gccggcaacc ttctacatcg
      121 tccacctgaa ggcccctggg atcgacgcga tcggcgccac ccttcccgga acgccgttcc
      181 tgacgctcgg gcgcaaccag aaggttggct gggccagcac caacacattc gcggacgttc
      241 aggacatcta tcttgaaaag ctggatccga acgatcccgg caagtacatg accccgcagg
      301 gcccggtcgc gttcgaaatc gaaaagcacg tgcttcgcga gcgcactgga cgtgacgaat
      361 acaaggaaca caagttcgac ctgcgataca cccgccacgg cgcggtgctt aacgatgcca
      421 tgattgatgg ccttccaggc cagccaatcg ccctgaagac cgcttccata tggccgaccg
      481 atgaatcgct ttccatgaag aagtacctta cggcgacgtc gattgacgac attttcaacg
      541 ccctgcagtc gtggggcctg ccgatccaga actgggtcgc ggtcgatgat gccggacaca
      601 tcggttactt cccgatgggg gtcgtgccga tccgccggac tttcgacggc accgttcccg
      661 ttccgggatg gacggacgaa tttgaatggg acggcttcat tccgtatgac cagttgccga
      721 agatgatcga cccgccgtcg ggcatgatcg tgaccgccaa caaccaggtc atgcccgccc
      781 aggattaccc gtatccctac agcatcgaca cgatgcaggg ataccgcgcc gggaggatcc
      841 gggaactgct gaaggccggc ggcaagtgga cggcggacgg cattcgaacc atgcagatgg
      901 acgcgcacgt tccccaggcc gatcgcctgc tgccgtcact gctgaaggcc cttgatggcg
      961 ccgacctgga cgacttcgaa aagtccgcgc ttgcaaggct gaagggatgg gacagggtcg
     1021 cggccatcga ttcggtcggc gccagcgtc
//
LOCUS       DAOPPA010000674         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_157799_length_1026_cov_6.373841, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000674 DAOPPA010000000
VERSION     DAOPPA010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157799_length_1026_cov_6.373841"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKH54_14135"
     CDS             complement(<1..726)
                     /locus_tag="PKH54_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01084.1"
                     /translation="MRGSNGILMALAAVLMAVCLPVGASARNDPAKRGMVAVFEIEVE
                     GVELSRSVVVNLTEYLSSRMTAVGGYKTVASNRIEKALVEKKLESHADACDKTCQIEL
                     AQKVSADKVLHAKIWKVGSVCSLTLDLYDLESEVMEKSVEVEAASCDQPHLKKAVAAA
                     VLKLGGAEAGQSSVQGGGPSVGLSEGGSAPDNNAPPPAVKAETGYLYVQGEPKGARVD
                     ISGPKSFGDKGRVATYLPVEPFEV"
BASE COUNT          226 a          335 c          265 g          200 t
ORIGIN      
        1 cacttcaaac ggctcgacag gcaggtatgt cgccaccctg cccttgtcac cgaacgactt
       61 cggtccgcta atgtcgacac gcgcaccctt cggttcgccc tgcacgtaca ggtaacctgt
      121 ttccgccttc accgccggtg gcggcgcgtt gttgtccggc gcagaaccgc cttccgataa
      181 accaaccgaa ggtccgccac cctgaaccga cgactgaccg gcttccgcgc cgcccagttt
      241 cagcacagcc gcagccaccg ccttcttcaa atgcggctgg tcacaggaag cagcttcgac
      301 ttcgaccgat ttttccatca cctcggattc gaggtcgtaa agatccagcg tcagggaaca
      361 cacggacccg accttccaga tttttgcatg aaggaccttg tccgcgctga ccttctgggc
      421 cagttcaatc tggcacgtct tgtcgcaggc gtcggcgtgc gattcaagct tcttttcaac
      481 aagcgccttt tcgatgcggt tcgacgccac tgtcttgtaa ccgcccaccg ccgtcatgcg
      541 cgacgacagg tactcggtca ggttaacgac gaccgacctg gacaattcaa cgccttcgac
      601 ctcgatttca aacacggcca ccatgccgcg tttcgccggg tcgttccggg ccgaagcccc
      661 gacgggcagg cagacggcca tcagaacggc ggccagcgcc ataagaattc cattacttcc
      721 acgcacaagg cacccccgtg atcagaaccc acagttgacg gatttgtacc acattccgaa
      781 agcaacgtca gtgtgggcga tccagaagca ggcctgcgtc aatgtcatcc atcgatttgt
      841 ccccagatta ctggtcaggg tcacttgccg caaagtatca ggccgagcat cccgacctcg
      901 cggaacgggc ccgaatcgag gcgaaaaatg ctctgaatgc ccaggaaaaa gagcggcagt
      961 cgataaagga tagcgtatag gaactttcat ccacaaaacg cctccatggg acgagtatgg
     1021 tcgggg
//
LOCUS       DAOPPA010000675         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_159015_length_1021_cov_4.562112, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000675 DAOPPA010000000
VERSION     DAOPPA010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159015_length_1021_cov_4.562112"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKH54_14140"
     CDS             complement(<1..527)
                     /locus_tag="PKH54_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01085.1"
                     /translation="MRRVSVSALLFLVVLAGCQAQDFPPPTAALSSPVLVPGFQVPDD
                     RLQNSPDAPDTPQTPDGEDVTADGSTGLDAAVSSFKAKNRMKVRRITGPDGKQIDEIV
                     VDGRPPEHFRMTATIIDRSAAATISGMPAFDWSYGCSATSAAMIAAYYDRNGFPNMYN
                     GPTNGGVMPLNNSSWG"
     gene            complement(604..>1021)
                     /locus_tag="PKH54_14145"
     CDS             complement(604..>1021)
                     /locus_tag="PKH54_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD01086.1"
                     /translation="VFELGQARIGISICYEDLLAPFHWKMSELDPNVIVNITNDAWFG
                     RTREPWLHLQLAELRSVETRMFMARATNTGVSAFVDPVGRRLAHTSMDDPEYLVADVA
                     LTREDTLYMRFGDWFAWACAAFVLAMAAACVTWRRD"
BASE COUNT          196 a          297 c          325 g          203 t
ORIGIN      
        1 ccccaggatg aattgttaag cggcatgacc ccgccgttgg tcgggccgtt gtacatgttg
       61 gggaacccgt tgcggtcgta gtatgcggca atcatcgcgg ccgacgtggc agagcaccca
      121 tacgaccagt cgaaagccgg cattccggaa atggttgccg ccgcggatcg gtcgatgatg
      181 gtcgcggtca tcctgaagtg ttccggcggg cgcccatcca cgacgatttc gtcgatctgc
      241 tttccatccg gcccggtgat gcggcggact ttcatgcggt ttttggcctt gaagctgctt
      301 accgcggcat cgagtccggt tgatccatcg gccgtgacat cctcgccgtc gggtgtctgc
      361 ggagtgtccg gggcgtccgg ggaattctga agtcgatcat ccgggacctg gaagcctggc
      421 acaaggactg ggcttgaaag ggccgcggtc ggcggcggga agtcctgcgc ctggcagccg
      481 gccaggacga caaggaaaag caatgcactt acagatactc gacgcatctt cgccaccgcc
      541 ggttcgtgaa aagtatttca agggaaggtc agcagtatct tttattccgg atgacgtgca
      601 aagtcaatcg cggcgccatg ttacgcaggc cgccgccatc gcaaggacga acgccgcgca
      661 ggcccacgcg aaccagtcgc cgaaccgcat atagagggtg tcttccctgg tcagggcaac
      721 gtcggcgacc aggtattcgg ggtcgtccat cgaagtatgg gccaggcgtc gtccaacggg
      781 atcgacgaat gccgaaacgc cggtgttggt cgcgcgcgcc atgaacatcc tggtttcgac
      841 gctgcgaagt tcggcaagct gaagatgcag ccatggctcg cgcgtccgcc cgaaccatgc
      901 gtcgttcgtg atgttgacga tcacgttcgg atccagttcg gacatcttcc agtggaacgg
      961 cgccagcagg tcttcgtaac agatggatat cccgattctt gcctggccca gctcgaagac
     1021 g
//
LOCUS       DAOPPA010000676         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_159058_length_1021_cov_2.819876, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000676 DAOPPA010000000
VERSION     DAOPPA010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159058_length_1021_cov_2.819876"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1021)
                     /locus_tag="PKH54_14150"
     CDS             complement(184..>1021)
                     /locus_tag="PKH54_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01087.1"
                     /translation="EGSKIMATPISGGPATELAEGTMSFLALTPDKTRLAGFKNNPTL
                     DNSNAIDLYSLETTGGELTLIAEGISGLYSNYPYPVFSVTPIRTQNMGWRIADDSTSI
                     YVLPRCDSYSLEECDMLRIMLDGSGTEVLMSITSFGMGGREDPWTVWEVNDARGFAMG
                     TIIVDQGTLAKSLIIHHIADGSNHTIDHAMAGGISAPDRSSFISWFDDPYVLTWYDPA
                     TLERRALTPYRPEGTTNVNGPIDFHFSDDSTKINYNALFGRSMTGYLPGARQRHYIVD
                     LD"
BASE COUNT          182 a          287 c          304 g          248 t
ORIGIN      
        1 tcgatgacat cgaaagcatc ctgaactccg ccgcgtgacc ggtcccggcg ggcgattttc
       61 ctgccctgag cccgccgaag ggcctgtcga agggtcattc gcccctaccg ttcggggtta
      121 gttcggccct tcgtcatttg tggacggatt tctgcgctac gttcttgtgc ggcaggactg
      181 tgttcagtcc agatccacga tgtaatgtcg ttggcgcgcg cccggcagat agccggtcat
      241 gctgcgcccg aacagtgcgt tgtaattgat tttcgtggaa tcgtcagaga agtggaaatc
      301 gatcggtccg ttgacatttg tggtgccttc gggcctgtag ggcgtcagcg cccgcctttc
      361 aagagtcgcc gggtcatacc aggtcaggac gtatgggtcg tcaaaccacg agatgaaaga
      421 gcttcgatcc ggggccgaga tcccgccggc catcgcgtgg tcgatggtat ggttcgaccc
      481 gtcggcaatg tggtggatga tcagcgattt ggcaagcgtg ccctggtcga caattatggt
      541 tcccatcgcg aagcccctgg cgtcgttgac ttcccagaca gtccagggat cttcacgtcc
      601 gcccattcca aaggatgtga tcgacatcag gacctcggtt cccgaaccat cgagcatgat
      661 ccggagcatg tcgcattctt ccagactgta tgaatcgcac cttggcagca cgtagatcga
      721 tgtcgagtcg tccgcgatcc tccatcccat attctgggtc cggatcggcg tcacgctgaa
      781 caccgggtac gggtagttgg aatacagtcc gctgatccct tcggcaatca gggtcagttc
      841 accgcccgtc gtttcaaggg agtacagatc gattgcgttg gagttatcaa gggttgggtt
      901 gtttttgaat cctgccagtc tggttttgtc tggtgtcagc gccaggaagc tcatcgtgcc
      961 ttcggcaagt tccgtcgccg gtccaccgct gatcggggtg gccatgatct tcgatccctc
     1021 g
//
LOCUS       DAOPPA010000677         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_159535_length_1019_cov_2.814315, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000677 DAOPPA010000000
VERSION     DAOPPA010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159535_length_1019_cov_2.814315"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1019)
                     /gene="hutU"
                     /locus_tag="PKH54_14155"
     CDS             complement(55..>1019)
                     /gene="hutU"
                     /locus_tag="PKH54_14155"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOD01088.1"
                     /translation="SIGLHGNCATVFPELVRRGIVPDLVTDQTSAHDMLTGYVPDQVD
                     YAEALALRKSDPDKYQKLSFDAVVRHVQAMLDLQRLGSHVFDYGNNIRTQAFRMGLAD
                     AYAFPGFVPAYIRPQFCSGRGPFRWAALSGDPADIAATDDLVLELFPKDEGLARWIDM
                     ARRKVAWQGLPARICWLGYGERDVAGQAFNDLVRRGKVKAPIVIGRDHLDSGSVASPH
                     RETEAMKDGSDIVADWPLLNAMLNASNGATWVSIHHGGGVGIGYSIHAGVVIVADGTD
                     AQARKLERVLRGDPATGVFRHVDAGYDLAVDAARRTALKIPHMV"
BASE COUNT          218 a          327 c          290 g          184 t
ORIGIN      
        1 gcataactga agtattacgg gatgataacg ctcagatcgt ggcggaactc cggactagac
       61 catgtgggga atcttcagtg cggttctgcg ggccgcatcg acggcaagat cgtagccggc
      121 atcaacgtgc cggaagacac cggtcgcggg atcgccacga agcacccttt ccagtttgcg
      181 ggcctgcgcg tccgttccgt ccgccacgat tacgacaccg gcatgaatgg aatatccgat
      241 gccgaccccg ccgccatgat gaatcgaaac ccatgtggcg ccgttggacg cgttcaacat
      301 cgcattcagc agcggccagt ccgcgacgat gtccgacccg tccttcattg cctcggtctc
      361 gcgatgcggc gaagccaccg atcccgaatc aaggtgatcg cgtccgataa cgataggcgc
      421 cttgaccttt ccacgtcgaa ccagatcgtt aaaggcctgg cccgcaacgt cgcgttcgcc
      481 atacccgagc cagcagattc gggcgggcag cccctgccag gcaaccttgc gccttgccat
      541 gtcaatccac ctggcaagac cttcgtcctt cgggaacagt tcaagcacaa ggtcgtccgt
      601 ggccgcgatg tcggcggggt cgccggaaag cgccgcccac ctgaacggtc cgcggcccga
      661 acagaactgc ggcctgatat aggcaggcac gaatccggga aatgcatagg catcggcaag
      721 ccccatgcgg aaagcctgcg tcctgatgtt attgccgtaa tcaaacacgt gcgacccgag
      781 gcgctgcaga tcaagcattg cctgaacgtg cctgacgacc gcgtcgaagg acaacttctg
      841 gtacttgtcg ggatcgctct tccgaagtgc cagcgcctct gcgtaatcaa cctgatccgg
      901 aacgtacccg gtcagcatgt catgagccga cgtctgatcg gtcaccaggt ctggcacgat
      961 tccccgccga accagttccg ggaaaactgt cgcacaattg ccgtgaagcc cgatggaca
//
LOCUS       DAOPPA010000678         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_159648_length_1019_cov_1.751037, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000678 DAOPPA010000000
VERSION     DAOPPA010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159648_length_1019_cov_1.751037"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKH54_14160"
     CDS             complement(<1..703)
                     /locus_tag="PKH54_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01089.1"
                     /translation="MKIYPEQLGNQVADKVDYEIFSHAWSARVRILLPVAEAAAVLKK
                     LWWHEGHSGWRRSSPAGGTRWDIKSAAWSTGRKPSSESGLGRRNDDWGTKIYNLSIQL
                     GKEGYCLDIGGIRRSDLDRVMTVVLGLGDRQWAVAYWRRLERYVVLTTADATSLSQAL
                     SGANEHRGGRESAKEYLLRGRMFPVTVRTRSKLQAEAVIYRVDPGRGTTNAWKLEVRL
                     KGQSSTATTFSEADAM"
BASE COUNT          203 a          325 c          276 g          215 t
ORIGIN      
        1 gcatggcgtc ggcttcactg aacgtagtgg ccgtcgatga ttggcctttc agacgcactt
       61 cgagtttcca ggcgttcgtg gttccacgcc cgggatcgac ccggtagatg accgcctctg
      121 cctgcagctt ggaacgggtt cggacggtca cggggaacat tctgccccgc aacaaatact
      181 ccttggcgct ttcccgcccg ccccgatgtt cgtttgcacc tgaaagcgcc tgcgacagac
      241 tggttgcatc agccgtcgta agcacaacat atctttccag gcgacgccag tacgccaccg
      301 cccactggcg atcgccaagg ccaaggacga ccgtcatgac ccggtccagg tcggatcgcc
      361 tgatgccgcc gatgtccagg caatagcctt cctttccaag ctggatcgac aggttgtaaa
      421 tcttggtgcc ccaatcgtcg ttgcgccgac cgagacctga ttcggaagat ggcttacgcc
      481 cagtgctcca ggctgccgac ttaatatccc acctggtgcc acccgcaggt gatgatcgac
      541 gccagcctga atgaccctca tgccaccaca atttcttcag aactgcggcc gcttctgcaa
      601 cgggcaacag tattcgcacg cgagcactcc aggcatggct gaagatttca taatcaacct
      661 tatcggcaac ctgattgccg agctgttcgg ggtatatttt catacgtgtt ctccacctcg
      721 gacacccggc ccgatcaggt tcgccagctg tcgggccggg ttccgggtca attactttct
      781 gacagacaca tcaccagttg cgtcgccatg cggaaacgag cctccctgtc ggcggcagac
      841 tgccgtacat gtcctgtggc gtttcagcgt atcggaaggc cacgggcctg atgtttccgc
      901 cgacaggaat tcttgagaaa gcgccggaac cagagatgcc agcgtcgacg atggggaccg
      961 aacggctttc gatatctccg atgacaatga ggaccccccc tggtcgaacc gcggtcatc
//
LOCUS       DAOPPA010000679         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Myxococcota bacterium isolate AMR_MDS_1560
            NODE_160693_length_1014_cov_3.279458, whole genome shotgun
            sequence.
ACCESSION   DAOPPA010000679 DAOPPA010000000
VERSION     DAOPPA010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854765
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Myxococcota bacterium (wastewater metagenome)
  ORGANISM  Myxococcota bacterium
            Bacteria; Myxococcota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:11:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,795
            CDSs (with protein)               :: 2,795
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 8 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Myxococcota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160693_length_1014_cov_3.279458"
                     /isolate="AMR_MDS_1560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818507"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKH54_14165"
     CDS             <1..476
                     /locus_tag="PKH54_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01090.1"
                     /translation="GLSKTIRRSHADSMEVTDTIMRRLLGAGIPLVERDRRLTDKLLE
                     ELHVSASELVDSETAPSIGHQLGVKTLIVGRFEFSGEFHYAQDENGDVILKKPKSIGY
                     QAVRIKALDVSRGQVIIDARFSMTGSVVSVLMPQTLARYAATEILSRLGAEGVAF"
     gene            502..>1014
                     /locus_tag="PKH54_14170"
     CDS             502..>1014
                     /locus_tag="PKH54_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD01091.1"
                     /translation="MADRDHDSSSLEPGVGKSAAVIGGVGLASAGAAVAAGVQVQNII
                     EAAREAAIQALTEHQHSLAEDGVARVGGTADEQTARLETLASELDARMATALTDLATG
                     LQQQISENIERLATHMTDQLNSTFHGGQKDLHRLAQELESDIIEQRDRAIAEIREEGQ
                     RAVELVQHQAD"
BASE COUNT          220 a          269 c          331 g          194 t
ORIGIN      
        1 acgggctttc caagaccatc aggcgctccc acgcggattc catggaagtg accgacacga
       61 taatgcgacg tcttcttggg gccggaattc ctctggtcga aagggatcgc cgtcttaccg
      121 ataagttgct ggaagaattg catgtttccg catctgaact ggtcgattcg gaaacggcgc
      181 cttccatcgg gcatcagctt ggcgtgaaga ccctgatcgt cggccgtttc gagttcagcg
      241 gcgaatttca ttatgcccag gacgaaaacg gcgatgtgat actgaagaag ccgaagtcga
      301 tcggttacca ggccgttcgg ataaaggctc ttgacgtgtc caggggccag gtgatcattg
      361 acgccaggtt cagcatgacc ggaagcgtgg taagcgtatt gatgccacag acactggcac
      421 ggtacgccgc gacagaaatc ctttcgcggc tgggcgctga aggtgtggct ttctgatttt
      481 gcaagtcggt ggaggtttga catggctgac agggatcacg actcaagttc gctggaaccc
      541 ggggttggaa agtctgcggc ggtaatcggc ggtgtcggcc tggccagcgc gggggcagcc
      601 gtggctgccg gggtgcaggt tcagaacatc atcgaggccg cccgtgaagc ggcaatccag
      661 gctttgaccg agcaccagca ttcgctggcg gaagacgggg ttgccagggt cggtggaacg
      721 gccgacgaac agaccgccag acttgaaacg cttgcaagcg aactggatgc caggatggcg
      781 actgccctga ccgatctcgc gaccggattg cagcagcaga tcagtgaaaa catcgagcgg
      841 ctggcgaccc acatgaccga ccagttgaat tcgactttcc acggcggtca gaaggatttg
      901 cacaggcttg cccaggagct tgagtcggat atcatcgagc agcgcgatcg cgcaatcgcc
      961 gagattcgcg aagaggggca gcgggccgtc gagctcgtgc agcaccaggc cgat
//
